LOCUS       DAESHI010000001        88076 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.001, whole genome shotgun sequence.
ACCESSION   DAESHI010000001 DAESHI010000000
VERSION     DAESHI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 88076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88076
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.001"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..903)
                     /gene="nac"
                     /locus_tag="KW011_000001"
     CDS             complement(<1..903)
                     /gene="nac"
                     /locus_tag="KW011_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HBH7658451.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1230..1305
                     /locus_tag="KW011_000002"
     tRNA            1230..1305
                     /locus_tag="KW011_000002"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Asn,seq:gtt)
     gene            complement(1361..2296)
                     /gene="ldtA"
                     /locus_tag="KW011_000003"
     CDS             complement(1361..2296)
                     /gene="ldtA"
                     /locus_tag="KW011_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBH7658452.1"
                     /translation="MMRRVNIFCSFALLFASHNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(2358..3437)
                     /gene="cobT"
                     /locus_tag="KW011_000004"
     CDS             complement(2358..3437)
                     /gene="cobT"
                     /locus_tag="KW011_000004"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBH7658453.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3449..4192)
                     /gene="cobS"
                     /locus_tag="KW011_000005"
     CDS             complement(3449..4192)
                     /gene="cobS"
                     /locus_tag="KW011_000005"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBH7658454.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4189..4734)
                     /gene="cobU"
                     /locus_tag="KW011_000006"
     CDS             complement(4189..4734)
                     /gene="cobU"
                     /locus_tag="KW011_000006"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBH7658455.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(4868..5305)
                     /locus_tag="KW011_000007"
     CDS             complement(4868..5305)
                     /locus_tag="KW011_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBH7658456.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5523..5698)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5787..6463)
                     /locus_tag="KW011_000008"
                     /pseudo
     CDS             complement(5787..6463)
                     /locus_tag="KW011_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
     gene            complement(6666..7475)
                     /gene="pduF"
                     /locus_tag="KW011_000009"
     CDS             complement(6666..7475)
                     /gene="pduF"
                     /locus_tag="KW011_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HBH7658457.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
     gene            7987..8271
                     /gene="pduA"
                     /locus_tag="KW011_000010"
     CDS             7987..8271
                     /gene="pduA"
                     /locus_tag="KW011_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HBH7658458.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            8379..9080
                     /gene="pduB"
                     /locus_tag="KW011_000011"
     CDS             8379..9080
                     /gene="pduB"
                     /locus_tag="KW011_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001777285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HBH7658459.1"
                     /translation="MAEKSCTLTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSI
                     GILGARIGAGPHIMAADEAVKATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKR
                     GIEVALKELDRTFGDVYANEAGHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAV
                     GVLMADTALKSANVDVVAYSSPAQGTSYSNEVILIISGDSGAVRQAVISAREIGKTVL
                     STLGDTPKNDHPSYI"
     gene            9099..10763
                     /gene="pduC"
                     /locus_tag="KW011_000012"
     CDS             9099..10763
                     /gene="pduC"
                     /locus_tag="KW011_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HBH7658460.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTDGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGGFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            10773..11441
                     /locus_tag="KW011_000013"
     CDS             10773..11441
                     /locus_tag="KW011_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HBH7658461.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            11456..11974
                     /gene="pduE"
                     /locus_tag="KW011_000014"
     CDS             11456..11974
                     /gene="pduE"
                     /locus_tag="KW011_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HBH7658462.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            11984..13816
                     /locus_tag="KW011_000015"
     CDS             11984..13816
                     /locus_tag="KW011_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HBH7658463.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            13806..14156
                     /locus_tag="KW011_000016"
     CDS             13806..14156
                     /locus_tag="KW011_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HBH7658464.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            14176..14451
                     /gene="pduJ"
                     /locus_tag="KW011_000017"
     CDS             14176..14451
                     /gene="pduJ"
                     /locus_tag="KW011_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HBH7658465.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            14475..14897
                     /locus_tag="KW011_000018"
     CDS             14475..14897
                     /locus_tag="KW011_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBH7658466.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            14900..15532
                     /locus_tag="KW011_000019"
     CDS             14900..15532
                     /locus_tag="KW011_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBH7658467.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            15529..16020
                     /gene="pduM"
                     /locus_tag="KW011_000020"
     CDS             15529..16020
                     /gene="pduM"
                     /locus_tag="KW011_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HBH7658468.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            16024..16299
                     /gene="pduN"
                     /locus_tag="KW011_000021"
     CDS             16024..16299
                     /gene="pduN"
                     /locus_tag="KW011_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HBH7658469.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            16311..17318
                     /gene="pduO"
                     /locus_tag="KW011_000022"
     CDS             16311..17318
                     /gene="pduO"
                     /locus_tag="KW011_000022"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HBH7658470.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            17315..18700
                     /gene="pduP"
                     /locus_tag="KW011_000023"
                     /pseudo
     CDS             17315..18700
                     /gene="pduP"
                     /locus_tag="KW011_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            18711..19823
                     /locus_tag="KW011_000024"
     CDS             18711..19823
                     /locus_tag="KW011_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBH7658471.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLEELL"
     gene            19820..21163
                     /locus_tag="KW011_000025"
     CDS             19820..21163
                     /locus_tag="KW011_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HBH7658472.1"
                     /translation="MNVGLISCDDQTIRERVQAAGVVGAGGAGFPTHIKLQARADTFL
                     VNAAECEPMLKVDQQLMAQQASRLLRGVHYAMKATGASSGIIALKEKYQRAINALTPL
                     LPPDIRLHILPDVYPAGDEVLTIWMATGRRVPPAALPVSVGVVVNNVQTVLNIARAVE
                     QQYPVTRRTLTVNGAVAKPITVTVPIGMSLREVLALAGGATVDAPGFINGGPMMGSLI
                     TSLDTPVSKTTGGLLVLPNSHSLIQRRMQNDRSVLAVAKTVCEQCRLCTDLCPRHLIG
                     HELAPHLLVRAVNYQQLATPQLLLTALTCSECNVCASVACPVGISPMRINRLLKQELR
                     AQNLRYEGALNPADPMANYRLIPVKRLVTRLGLTPWYQDAPLSEQVPQPEKVTLLLRQ
                     HIGASAIACVQKGDRVVHGQCVGQIPHGTLGAPIHASIDGMVSDVTENAITLVRG"
     gene            21166..21720
                     /gene="pduT"
                     /locus_tag="KW011_000026"
     CDS             21166..21720
                     /gene="pduT"
                     /locus_tag="KW011_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HBH7658473.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            21720..22070
                     /gene="pduU"
                     /locus_tag="KW011_000027"
     CDS             21720..22070
                     /gene="pduU"
                     /locus_tag="KW011_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HBH7658474.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            22075..22518
                     /locus_tag="KW011_000028"
     CDS             22075..22518
                     /locus_tag="KW011_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HBH7658475.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(22619..22948)
                     /locus_tag="KW011_000029"
     CDS             complement(22619..22948)
                     /locus_tag="KW011_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HBH7658476.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(23120..24178)
                     /locus_tag="KW011_000030"
     CDS             complement(23120..24178)
                     /locus_tag="KW011_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBH7658477.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(24376..24849)
                     /gene="sbmC"
                     /locus_tag="KW011_000031"
     CDS             complement(24376..24849)
                     /gene="sbmC"
                     /locus_tag="KW011_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HBH7658478.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(24968..26134)
                     /gene="dacD"
                     /locus_tag="KW011_000032"
     CDS             complement(24968..26134)
                     /gene="dacD"
                     /locus_tag="KW011_000032"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HBH7658479.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            26343..27770
                     /gene="sbcB"
                     /locus_tag="KW011_000033"
     CDS             26343..27770
                     /gene="sbcB"
                     /locus_tag="KW011_000033"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HBH7658480.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(27813..28040)
                     /locus_tag="KW011_000034"
     CDS             complement(27813..28040)
                     /locus_tag="KW011_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HBH7658481.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(28054..29112)
                     /gene="yeeE"
                     /locus_tag="KW011_000035"
     CDS             complement(28054..29112)
                     /gene="yeeE"
                     /locus_tag="KW011_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HBH7658482.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(29291..30649)
                     /gene="plaP"
                     /locus_tag="KW011_000036"
     CDS             complement(29291..30649)
                     /gene="plaP"
                     /locus_tag="KW011_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HBH7658483.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(30639..30701)
                     /gene="yoeI"
                     /locus_tag="KW011_000037"
     CDS             complement(30639..30701)
                     /gene="yoeI"
                     /locus_tag="KW011_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HBH7658484.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(30916..31845)
                     /locus_tag="KW011_000038"
     CDS             complement(30916..31845)
                     /locus_tag="KW011_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH7658485.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(31891..32715)
                     /locus_tag="KW011_000039"
     CDS             complement(31891..32715)
                     /locus_tag="KW011_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBH7658486.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(32798..33052)
                     /gene="yoeB"
                     /locus_tag="KW011_000040"
     CDS             complement(32798..33052)
                     /gene="yoeB"
                     /locus_tag="KW011_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HBH7658487.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(33049..33300)
                     /gene="yefM"
                     /locus_tag="KW011_000041"
     CDS             complement(33049..33300)
                     /gene="yefM"
                     /locus_tag="KW011_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HBH7658488.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            33583..33633
                     /gene="hisL"
                     /locus_tag="KW011_000042"
     CDS             33583..33633
                     /gene="hisL"
                     /locus_tag="KW011_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HBH7658489.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    33609..33732
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            33779..34678
                     /gene="hisG"
                     /locus_tag="KW011_000043"
     CDS             33779..34678
                     /gene="hisG"
                     /locus_tag="KW011_000043"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBH7658490.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            34684..35988
                     /gene="hisD"
                     /locus_tag="KW011_000044"
     CDS             34684..35988
                     /gene="hisD"
                     /locus_tag="KW011_000044"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBH7658491.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            35985..37055
                     /gene="hisC"
                     /locus_tag="KW011_000045"
     CDS             35985..37055
                     /gene="hisC"
                     /locus_tag="KW011_000045"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBH7658492.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            37055..38122
                     /gene="hisB"
                     /locus_tag="KW011_000046"
     CDS             37055..38122
                     /gene="hisB"
                     /locus_tag="KW011_000046"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HBH7658493.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            38122..38712
                     /gene="hisH"
                     /locus_tag="KW011_000047"
     CDS             38122..38712
                     /gene="hisH"
                     /locus_tag="KW011_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBH7658494.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            38712..39449
                     /gene="hisA"
                     /locus_tag="KW011_000048"
     CDS             38712..39449
                     /gene="hisA"
                     /locus_tag="KW011_000048"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBH7658495.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            39431..40207
                     /gene="hisF"
                     /locus_tag="KW011_000049"
     CDS             39431..40207
                     /gene="hisF"
                     /locus_tag="KW011_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBH7658496.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            40201..40812
                     /locus_tag="KW011_000050"
     CDS             40201..40812
                     /locus_tag="KW011_000050"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBH7658497.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(40909..41886)
                     /gene="wzzB"
                     /locus_tag="KW011_000051"
     CDS             complement(40909..41886)
                     /gene="wzzB"
                     /locus_tag="KW011_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HBH7658498.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(42032..43198)
                     /gene="ugd"
                     /locus_tag="KW011_000052"
     CDS             complement(42032..43198)
                     /gene="ugd"
                     /locus_tag="KW011_000052"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HBH7658499.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(43448..44854)
                     /gene="gndA"
                     /locus_tag="KW011_000053"
     CDS             complement(43448..44854)
                     /gene="gndA"
                     /locus_tag="KW011_000053"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBH7658500.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(45018..46388)
                     /gene="cpsG"
                     /locus_tag="KW011_000054"
                     /gene_synonym="manB"
     CDS             complement(45018..46388)
                     /gene="cpsG"
                     /locus_tag="KW011_000054"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HBH7658501.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(46493..47929)
                     /gene="cpsB"
                     /locus_tag="KW011_000055"
     CDS             complement(46493..47929)
                     /gene="cpsB"
                     /locus_tag="KW011_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HBH7658502.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(47932..49155)
                     /gene="wcaI"
                     /locus_tag="KW011_000056"
     CDS             complement(47932..49155)
                     /gene="wcaI"
                     /locus_tag="KW011_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HBH7658503.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(49152..49631)
                     /gene="gmm"
                     /locus_tag="KW011_000057"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(49152..49631)
                     /gene="gmm"
                     /locus_tag="KW011_000057"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HBH7658504.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(49634..50599)
                     /gene="fcl"
                     /locus_tag="KW011_000058"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(49634..50599)
                     /gene="fcl"
                     /locus_tag="KW011_000058"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HBH7658505.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(50602..51723)
                     /gene="gmd"
                     /locus_tag="KW011_000059"
     CDS             complement(50602..51723)
                     /gene="gmd"
                     /locus_tag="KW011_000059"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBH7658506.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(51749..52297)
                     /gene="wcaF"
                     /locus_tag="KW011_000060"
     CDS             complement(51749..52297)
                     /gene="wcaF"
                     /locus_tag="KW011_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HBH7658507.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(52313..53059)
                     /gene="wcaE"
                     /locus_tag="KW011_000061"
     CDS             complement(52313..53059)
                     /gene="wcaE"
                     /locus_tag="KW011_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HBH7658508.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(53070..54287)
                     /gene="wcaD"
                     /locus_tag="KW011_000062"
     CDS             complement(53070..54287)
                     /gene="wcaD"
                     /locus_tag="KW011_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HBH7658509.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(54262..55479)
                     /gene="wcaC"
                     /locus_tag="KW011_000063"
     CDS             complement(54262..55479)
                     /gene="wcaC"
                     /locus_tag="KW011_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HBH7658510.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(55476..55964)
                     /gene="wcaB"
                     /locus_tag="KW011_000064"
     CDS             complement(55476..55964)
                     /gene="wcaB"
                     /locus_tag="KW011_000064"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HBH7658511.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(55967..56806)
                     /gene="wcaA"
                     /locus_tag="KW011_000065"
     CDS             complement(55967..56806)
                     /gene="wcaA"
                     /locus_tag="KW011_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HBH7658512.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(56899..59061)
                     /gene="wzc"
                     /locus_tag="KW011_000066"
     CDS             complement(56899..59061)
                     /gene="wzc"
                     /locus_tag="KW011_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HBH7658513.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(59064..59507)
                     /gene="wzb"
                     /locus_tag="KW011_000067"
     CDS             complement(59064..59507)
                     /gene="wzb"
                     /locus_tag="KW011_000067"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HBH7658514.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(59513..60652)
                     /gene="wza"
                     /locus_tag="KW011_000068"
     CDS             complement(59513..60652)
                     /gene="wza"
                     /locus_tag="KW011_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HBH7658515.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            60961..61110
                     /locus_tag="KW011_000069"
     CDS             60961..61110
                     /locus_tag="KW011_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7658516.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            61311..62894
                     /locus_tag="KW011_000070"
     CDS             61311..62894
                     /locus_tag="KW011_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBH7658517.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(63168..65021)
                     /gene="asmA"
                     /locus_tag="KW011_000071"
     CDS             complement(63168..65021)
                     /gene="asmA"
                     /locus_tag="KW011_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HBH7658518.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(65043..65624)
                     /gene="dcd"
                     /locus_tag="KW011_000072"
     CDS             complement(65043..65624)
                     /gene="dcd"
                     /locus_tag="KW011_000072"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HBH7658519.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(65716..66357)
                     /gene="udk"
                     /locus_tag="KW011_000073"
     CDS             complement(65716..66357)
                     /gene="udk"
                     /locus_tag="KW011_000073"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBH7658520.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            66675..69992
                     /locus_tag="KW011_000074"
     CDS             66675..69992
                     /locus_tag="KW011_000074"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBH7658521.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(70102..70950)
                     /gene="alkA"
                     /locus_tag="KW011_000075"
     CDS             complement(70102..70950)
                     /gene="alkA"
                     /locus_tag="KW011_000075"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HBH7658522.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            71084..72436
                     /gene="yegD"
                     /locus_tag="KW011_000076"
     CDS             71084..72436
                     /gene="yegD"
                     /locus_tag="KW011_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBH7658523.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(72449..74389)
                     /gene="yegI"
                     /locus_tag="KW011_000077"
     CDS             complement(72449..74389)
                     /gene="yegI"
                     /locus_tag="KW011_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HBH7658524.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVVWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(74386..75147)
                     /gene="pphC"
                     /locus_tag="KW011_000078"
     CDS             complement(74386..75147)
                     /gene="pphC"
                     /locus_tag="KW011_000078"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HBH7658525.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(75144..75803)
                     /locus_tag="KW011_000079"
     CDS             complement(75144..75803)
                     /locus_tag="KW011_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBH7658526.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(76024..76083)
                     /gene="ibsA"
                     /locus_tag="KW011_000080"
     CDS             complement(76024..76083)
                     /gene="ibsA"
                     /locus_tag="KW011_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HBH7658527.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(76356..76412)
                     /locus_tag="KW011_000081"
     CDS             complement(76356..76412)
                     /locus_tag="KW011_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBH7658528.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(76683..76739)
                     /locus_tag="KW011_000082"
     CDS             complement(76683..76739)
                     /locus_tag="KW011_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBH7658529.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            77018..78259
                     /gene="mdtA"
                     /locus_tag="KW011_000083"
     CDS             77018..78259
                     /gene="mdtA"
                     /locus_tag="KW011_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HBH7658530.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            78259..81381
                     /gene="mdtB"
                     /locus_tag="KW011_000084"
     CDS             78259..81381
                     /gene="mdtB"
                     /locus_tag="KW011_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HBH7658531.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            81382..84459
                     /gene="mdtC"
                     /locus_tag="KW011_000085"
     CDS             81382..84459
                     /gene="mdtC"
                     /locus_tag="KW011_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HBH7658532.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            84460..85875
                     /locus_tag="KW011_000086"
     CDS             84460..85875
                     /locus_tag="KW011_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH7658533.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFHLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            85872..87275
                     /gene="baeS"
                     /locus_tag="KW011_000087"
     CDS             85872..87275
                     /gene="baeS"
                     /locus_tag="KW011_000087"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HBH7658534.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            87272..87994
                     /gene="baeR"
                     /locus_tag="KW011_000088"
     CDS             87272..87994
                     /gene="baeR"
                     /locus_tag="KW011_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HBH7658535.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
BASE COUNT        21549 a        23315 c        22152 g        21060 t
ORIGIN      
        1 ccactgcctt ttttccatca ctggggaact aatcactgac attaacaact ctttcactgc
       61 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
      121 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
      181 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
      241 agactcaatt tcaccaatga cttttgccgt gaatcgccgt aaggaaaaag cctcatcaac
      301 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
      361 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
      421 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
      481 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
      541 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
      601 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
      661 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
      721 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
      781 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
      841 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
      901 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
      961 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     1021 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     1081 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     1141 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     1201 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     1261 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     1321 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     1381 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     1441 catcaacttc ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     1501 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     1561 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     1621 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     1681 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     1741 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     1801 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     1861 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     1921 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     1981 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     2041 gaatggtgag ttgcgagcca gacttcggca aaaaaacatc agcgcccggg ttcgcttcca
     2101 gcatgttact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc tgggtattgt
     2161 gatcaggaac agttacagta agcgactgcc ccactaaacg gctaccctct ggaggtaatg
     2221 gataagttac cgccaggcta ttatggctgg caaaaagcag agcaaatgag caaaaaatat
     2281 ttacacgacg catcatgtcc ctttcctaag tcgcgaaagc tatccgttaa gtatagcttt
     2341 tatcagactt ttcgttttta actgttcaaa tcagaagtcg tattccccgg tagaacaata
     2401 ttactggcag caagttcgcc catgttgtta tatatcgcac aggcagcttc gatgatgggc
     2461 atcgccagag ctgcgccact cccctcacct aaacgcatct ccatattgag ataaggctcc
     2521 agccccaaat gcgacagcgc aatacgcgcc cctttttctg ccgacaggtg agaaggaatg
     2581 agatacggtt tgattgcagg agacatctgg caggctgcga gcgcggcagc ataagaaaga
     2641 aatccatcca gcaacacggg taaaccgcag gaagcagcgc ctaacatcac gccagccatt
     2701 ccgaccaaat caaatccacc cacttttgcc aggacatcaa cgccatcctg cggatttggt
     2761 tgattcaacg taatcgcccg gcgcacaaca tcaattttat tagccagttt atctgtcggc
     2821 aggtttgcgc caatcccaac cacttcttca ggagcccggc cagtgattgt gctgactatt
     2881 gccgctgccg gtgtcgtgtt tgccatcccc aggtcaccta caccaaacag cgtaacaccg
     2941 ttttttgcca gctcccgcgt ataacatatg acgtccaaaa gcaacttttc tgccagatgg
     3001 cgactcattg ccggagctga agcaatattg ccactacctc gagcaacacg catgttgata
     3061 agcccgggga taggctcagc agtatcaata ccaacatcaa ttacgtagac gttagcgccc
     3121 gcttgtgctg ccagcacaca cacgccggtt gttccgcggg tcatattttc agcctgaatg
     3181 gctgtcactt cttttgggga aatagcgacc ccttcctccc agacgccgtg atcggcacac
     3241 atgaccagta ccgctttttt gcccacatgc ggaacgccat tcaaccccgg cattcctgcc
     3301 agttgtatgg caagcgcctc cagccttccc aggctaccaa caggtttgag taacccgtcg
     3361 atatgccgtt gtgcacgcga catagcggca ggatcaatag cagggatcgt attcagtaaa
     3421 tcggcaagtg tttgcatgtc acgtctcttt atagcagtgc cagcaggaag accagttcac
     3481 caagctctat agcagctccc agcgtatcgc cggtttgccc gcccagcgta cgtttgagaa
     3541 gctgaccgag gatgaaaatc gccaccatcg tcaccaccat agcagccaca ccatgcatac
     3601 ccggcaataa tacggcagca aaaatagcgg ctaagccgag tgtgacgcag gtttgtcgcc
     3661 catcaatttt gccaatgaac acattgccaa gtccttcttc ccgggcataa cgatgacgat
     3721 acatcaataa tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc gatgcaagga
     3781 tcggttcgcc acgcagagcc aactcgctta ataccagaat ctttgccagt acaacaaaaa
     3841 tcaatgccag accgccgtgg gtgcctaaac ggctatcacg catgatttcc aacatgcgat
     3901 cgcggctacg tgcagaaaat acgccatcac aggtatcggc gaggccgtcc aggtggaatc
     3961 ccccggtcat cagcgccagc acaagtacgc taaacagtgc cgccagtggt acgccacacc
     4021 atgcctgcag caccatgaag accagcccgc taatcgcgcc aagcaataat ccaattaaag
     4081 gaaaagtaat aataccgcga gaataatgct cgaaatccag tccctgggac cagcgacgcg
     4141 gtacaggcag acgcgtaata aaagagagca tcgcccaaaa taatttactc atttgatttt
     4201 tactccaata cccgaaacca ccaaccatac ttcatttgcc gcagcggcca gctgctgatt
     4261 tacccgcccg gcaatatcac gaaaatgtcg tgccagacga ctctccggca caatccccat
     4321 tcccacttcg ttagtcacta atacaacctt tgcgggacaa cgttggcagg cagcaatcaa
     4381 cgactgaatc tcagcattaa tcgcctgttc catcgcctga taatcccatt catcagggtc
     4441 tttatcgccg ccataatcaa acaacagatt agtcaccatg gtggtaacgc attccagcaa
     4501 tacagcctca tgcgggttaa tgtctgcatg aattaattca tcaagatgtt gccagcgctc
     4561 cactgtgtgc cagtgcgccg ggcggccttg ccgatgatgt tctatccgtg cagccatctc
     4621 atcatcaagg atttgtgagg tggcgatata cagaacctgc gaagagtccc caataagcgc
     4681 ctctgcatgg cgactcttcc cgctccgtgc gccccccgtc accaaaatca tcataccgac
     4741 tcctgattca attgtatgat ggtattcatt gaacagaaca atggatcccc caccgttgct
     4801 gaccgcaaaa catttacgcg attaatactg cgcgtaatat aattaaaaat ccagcattct
     4861 caatccatta cgaaagaaga tggcattgcg gaatagccag acttcctcgt gttaacgtcc
     4921 gacaaaatgc gtcattgaca gcggtttctc tggaatgaca gatttcattc agataatgga
     4981 ttaatgctac agccccacca gccggaccag tcagcaaacg ggcattgtgt tctgcaatca
     5041 aaattaccgt cgttccctca gaatgagcga gccattctgg tacggatatc tcgctttcag
     5101 ctgctatcaa tagcagcact gaataatcgc tatacgcatc tgataacgat tgcacaggcc
     5161 caccatcaag aggctggaaa tcttgctgcc agtcctcacc aacaaaacag gttattggca
     5221 atatttcagc cagatgtttc gccagatcca catctccaga taagaggcaa aaaagagcta
     5281 tcgattcggg ttttatcgtt ttcatgcctc tcgccctcca cttaatttcc tgtaatggcg
     5341 gtctggttga taagccatga aacaatattg ccacaccttg aatgcagccc tggtgcctgt
     5401 cggcactgcg atgaaagaac taaatgatgc cgccataccc tgatcctttc agggtggtag
     5461 gggtataaag acgcgccagt actctaccgg acgcttccgg gcaacccact tcccaccgaa
     5521 gttgttggta ttgactgaca gactggtctt ctggcttagc gtcatcctca cccgaccttc
     5581 ccaatctcgc gatcagtggc attttcgggt ttgtcagcat tacagcagcg ggggctgcga
     5641 gggattttca ccctcttccc aggcacaaat gtgccacacc tgtcagcgta tgttatgagc
     5701 tgatttcact tttcccataa cacgtaaata acgttaatta ttcattccag cgtttattta
     5761 aaccttccct ttttttagca tttattctat accgtttcca tcgccgcatt ttcactcact
     5821 tgctgtcgat acacctgcgg cgtaacctga taagtctgac ggaaaacttt gcaaaaataa
     5881 ctgtattgtc gtggggatta ggcgcacgag tctggcgcac cgaatctgac cgagatttgt
     5941 tctctttaat aaccacaaaa ttgagttgtt ttttgaggca attttccaca atcaacttca
     6001 acaaatcagc agaggccata actcttgagt aatccatctc aggaactttg cggaattcat
     6061 taagtaattc gggatctgcc tgccagcaat catcgacatt cagaatatcg accaaatcga
     6121 catcatcatt acgcagacga acttgcccgc acaatacaaa accgactaaa tgtccggcga
     6181 ttaccagtgg aatagagaaa tcggttaatc cggcatgaca gcgataaata caaggctgat
     6241 cggatttaga ggcttccagg cccccacaac gatcgctcat gcgacatcga gtgcttagct
     6301 gagggtgttg ccgcatcatc tgacaaaatg gagtgaagtt aaacagctcg gaaatttcat
     6361 cgccgtgaat attgaccacg acaaccgcaa ggctggtcgc ctgcgcaaaa tcctgcgcga
     6421 ttttattaat gagttctgaa ttaagtgtcg ccgcagaaat catgattaaa cctcccagtt
     6481 aatttattgt tatagcaaaa caattgtttt acatttaatc ttcacatcat ccctgatgat
     6541 ttactgacga gaagcacaaa ggacaataac aaaagcctcc gtgaagcggg agacttttta
     6601 ataagttcgg aaagcagatc acaaacagta aaaaaataaa tgtttataac tgccagagta
     6661 tttcgttatt cgctaattcc tttaggtgta acactattga caggtaaatt ctttgcaatg
     6721 agatagcgat aaatcgccgc accaacacat gccccaatga tcggtgcaat aatgggtaca
     6781 atgaaataag gaatattgcg tccaccagtc atcgcaattt ctccccaccc cgcaagccag
     6841 gtgaaaatct taggtccaaa atcacgcgct ggattcatgg caaaaccggt taatggtccg
     6901 gtagaagcac caataacagc aaccagaata ccaataagta aaggtgcaag cggtcctttg
     6961 ggtacgccat taccgtcatc ggtcaacgcc ataatcaacc ccattaacat ggacgttatg
     7021 accacttcga ccaacgcagc ttgccataca ttaatcgccg ctgccggata agtactgaag
     7081 atactggcga gttgtaaact ttctgcgcta ccacgcacca tattgtgagc agattcaaaa
     7141 tcggtaaaca gactactgta tagagaatat gccagaactg caccaccaaa ggcacccgct
     7201 aattgcgcca gacaaaatgg cagcacttta cgggctggga aagaggcaaa cagccacaga
     7261 gcaatggtaa ctgccgggtt gagatgagcg ccggaaatac ctgccgtaag gtaaacagca
     7321 agcgaaatcc ctaaccccca aatgatacaa atttcccaca gtcccagact tgccccggca
     7381 actttcaggg cgcacaaaca accaatgcca aaaaagagga acagtccggt gcccaaaaac
     7441 tcggcaacgc attgcgcctt gagtgaatca ttcatagtga cacctttttt acggagtttt
     7501 tctttatcag ccaactatgc gctgactgtt gtatataccg agcacaacta taaaaaattg
     7561 ctctgcagaa aatattgcca acatgcatga cagactcgtg taaaaatcgt gctaacataa
     7621 caaattattg tcattaccga atcatgcaac acagcgttag tcagtgttga aagtaataat
     7681 tcccaccaaa cttttacaat aaaaaattgc catcatgaca taataaaatt gaaacaaata
     7741 tcacagttcc ttcttcgaac taaactcaac caaatattac tcgcgattaa actcactagc
     7801 caacgcaatt aattcacatt gaaatatcca caaaacaacc aataaattaa caacatatag
     7861 ttcataaaag caattttata atatttgttc tctattaatt tctctcatta gccgattaag
     7921 tcagccattc tttttcctcg ctttttttta tagtcatatc gtcaaaaaaa tccaggcgag
     7981 gtctttatgc aacaagaagc gttaggaatg gtagaaacca aaggcttaac tgccgccata
     8041 gaggccgcag acgccatggt gaagtcggca aatgttttgc tggtcggcta cgaaaaaata
     8101 ggttccgggc tggtgaccgt catcgttcgt ggtgatgtcg gggcggtaaa agcggcaaca
     8161 gacgcgggtg ccgcagctgc gcataacgtc ggcgaagtaa aagccgtaca cgttattccc
     8221 cgcccacata ccgatgtcga aaaaatctta ccaaagggaa ttaacccatg agcagcaatg
     8281 aactggtcga acagatcatg gcgcaggtca tcgctcgcgt agcgacgcca gagcatgagg
     8341 ttctcccgaa aaccactcat caaatacgat agacggctat ggcagaaaaa agctgcacgc
     8401 taacagaatt tgttggcacc gctattggcg acactgtagg tctggtcatt gcgaacgttg
     8461 atagcgccct gttggacgcg atgaaacttg aaaaacgcta tcgttccatt ggcattctgg
     8521 gtgcgcgtat tggcgcaggg ccacacatta tggccgctga tgaagcggta aaagccacca
     8581 acactgaagt tattagcatt gaacttcctc gcgatactaa aggcggtgcc ggacacggct
     8641 cactgatcct cttaggtggc aacgatgttt ccgacgtgaa acgcggtatt gaagtcgccc
     8701 tgaaagaact cgatcggacc tttggcgatg tctatgccaa cgaagccgga catcttgaac
     8761 tgcaatatac cgcccgcgcc agctatgcac tggaaaaagc gtttggtgca ccactgggac
     8821 gggcttgcgg cgttattgtc ggcgctccgg ctgctgtggg tgttctgatg gccgacaccg
     8881 cgctgaagtc cgccaatgtg gatgttgtgg catacagctc cccagctcaa ggcaccagct
     8941 acagcaatga agtcattctg attatctcgg gtgactccgg cgcagtccgc caggcggtca
     9001 tctccgcacg cgaaatcggt aaaaccgtgc tttcgactct cggcgataca ccgaaaaacg
     9061 atcacccttc ctacatctga attctctgag gcagatttat gagatcgaaa agatttgaag
     9121 cactggcaaa acgcccggtc aaccaggacg gtttcgttaa agagtggatt gaagaaggct
     9181 ttattgcgat ggaaagcccg aacgatccta aaccgtcaat caaaattgtt aacggcgtcg
     9241 ttaccgaact ggatggaaaa cccgccagcc agtttgacct gatcgaccac tttatcgccc
     9301 gctacggcat caatctcgcg cgcgccgaag aagtcatcgc gatggattcg gtaaaacttg
     9361 ccaatatgtt gtgcgatcca aatgtcaaac gtagcgatat cgttcccctt accacagcaa
     9421 tgacgcctgc aaaaatcgtc gaagtggttt cgcagatgaa cgtggtagag atgatgatgg
     9481 cgatgcagaa aatgcgcgcc cgtcgaacac catcacaaca ggctcacgtc accaacgtca
     9541 aagataaccc agtacaaatt gccgccgacg cagctgaagg cgcatggcgc gggtttgatg
     9601 aacaggaaac caccgttgcc gttgctcgct atgcaccgtt caacgccatc gccctgctgg
     9661 tcggctcaca ggtgggacgt ccgggcgtct tgacccagtg ttcactggaa gaagccaccg
     9721 aactgaaact cggtatgtta ggccacacct gttacgccga aacaatttct gtctatggta
     9781 cagaacctgt tttcaccgat ggtgatgata ccccgtggtc aaaagggttt ctcgcgtcat
     9841 cctatgcttc tcgtggctta aaaatgcgct ttacttccgg ctccggctct gaagtacaga
     9901 tgggttacgc ggaaggcaaa tcaatgcttt acctcgaggc tcgttgcatt tacatcacca
     9961 aagccgcagg cgttcagggt ctgcagaatg gttcggtaag ctgcatcggt gttccatcag
    10021 ctgtgccatc aggcatccgc gcagtattag cggaaaactt aatttgctca tcgctggatc
    10081 tggaatgcgc ctccagtaat gaccagacct ttacccactc ggatatgcgc cgtaccgcac
    10141 gtttcctgat gcaatttctg ccaggcaccg actttatctc ttccggtttt tccgctgtgc
    10201 cgaactacga caacatgttt gctggttcaa acgaagatgc tgaagacttc gatgattaca
    10261 acgtcattca gcgtgacctg aaggttgatg gtgggctgcg accagtacgc gaagaagatg
    10321 ttatcgctat ccgcaataaa gccgctcgcg ctttacaggc tgtctttgcc ggtatgggac
    10381 taccgcctat taccgatgaa gaagtcgaag cggcaaccta tgcccacggt tcaaaagata
    10441 tgcctgagcg caatattgtg gaagacatca agttcgccca ggagattatc aataaaaacc
    10501 ggaacagcct ggaagtggtg aaagccttgg cgcaaggcgg ttttaccgat gtcgcccagg
    10561 acatgctcaa catgcaaaaa gcaaaactga ccggcgatta tctccatacc tcagccatca
    10621 tcgtcgatga cggacaagtg ctctctgcgg tcaatgacgt caatgattac gccggaccgg
    10681 ctacaggtta ccgcctgcaa ggggaacgct gggaagagat caaaaatatt cccggtgcac
    10741 ttgatcccaa cgaaattgac taagggtgag aaatggaaat taacgaaaaa ttactgcgtc
    10801 agattatcga agacgtgctg gcagaaatgc aacccagcga caagtccgtt tcatttcgcg
    10861 cacctgtctc tgcaactgtc ccttccgcgc cagataccgg aaattttctg accgaaattg
    10921 gcgaagccca acaaggcact cagcaagatg aagtcattat tgctgtaggc ccggcgttcg
    10981 gcctggcgca aaccgtcaat atcatcggta ttccgcataa aaacatcttg cgcgaagtga
    11041 ttgccggtat cgaagaagaa ggcattaaag cccgtgtaat tcgctgcttt aaatcgtccg
    11101 atgtcgcatt cgtagcagtc gaaggcgatc gtcttagtgg ttcaggtatt gctatcggta
    11161 ttcagtcgaa aggcacaacg gttatccacc agcagggact tcctcccttg tctaacctgg
    11221 aacttttccc acaagcgcct ttgttgacgc tggatactta tcgtcagatt ggcaaaaacg
    11281 ccgcgcgtta tgcgaagcgc gagtctccac aaccggtgcc gaccctcaac gatcaaatgg
    11341 cgcggccgaa ataccaggcc aaatctgcca ttttgcatat taaagagacg aagtacgtgg
    11401 tcactggcaa aaaaccgcaa gaactgcgcg tgacgtttta acaaaggaaa ccttcatgaa
    11461 taccgatgca attgagtcga tggtcaggga cgtgttgaac cgcatgaaca gtttacagga
    11521 tgcagctcct gtttctgcgg tccccaacgc atcaatactg agcgccaaag tgacggatta
    11581 cccgctggca aataaacatc cggaatgggt taaaacagcg accaataaaa cgctcgacga
    11641 tttcactctg gaaaatgtgc taagcgataa cgtcaccgcc ctggatatgc gcattacccc
    11701 tgaaacattg cgtattcagg ctgcgattgc cagagatgct ggtcgcgatc gtctggcgat
    11761 gaattttgaa cgcgccgctg agcttacctc agtaccagat gatcgcatcc tcgaaatcta
    11821 caatgcgtta cgcccttatc gttcaactaa gcaagaactt attgccattg ctgacgatct
    11881 tgaacagcgt tatcaggcca aaatttgtgc tgcatttgtc cgtgaagctg cggaacttta
    11941 tgtcgagcgc aaaaaactga aaggcgacga ttaaggacga actatgcgat atatagcagg
    12001 cattgatatc ggcaactcat caacagaggt tgcgctggca acgctcagtg caactggaga
    12061 gttgtcgttc gtgagtagcg ccctggagga aaccaccgga atcaaaggta cgttgcgtaa
    12121 cgtccatggt attcaggagg cgctcgcaca ggcaacgaaa aaagtcggca tcaatgttag
    12181 cgatatttcg cttattcgta tcaacgaagc aactccggtc attggtgatg tggcgatgga
    12241 aaccatcact gaaactatca tcaccgaatc aaccatgatc ggtcacaacc cgaaaactcc
    12301 gggcggagtg gggctgggcg tagggttcac catcactccg caagagttat taacacgccc
    12361 ggcggacaca ccttacatcc tggtggtgtc atcggcattt gattttgccg atatcgcgac
    12421 gatgataaac gcctccgtac gcgccggata tcagctgact ggcgttattc tgcaacgcga
    12481 cgatggcgta ctggtcaata accgcctgga aattccttta cctattgtgg atgaagtcct
    12541 ctacatcgat cgtattccgc tcggcatgct ggcagcaatt gaagttgccg ttcccgggaa
    12601 agttatcgaa accctgtcta acccgtatgg gattgccacc gtattcgccc tcaatgctga
    12661 agagactaaa aatattgttc ctgtcgcgcg ggcgctaatc ggcaaccgtt cagcagtggt
    12721 agtgaaaacg ccctctggcg acgttaaagc ccgttcgata cccgccggaa atatcgaact
    12781 gctttctgcc gggcgcacaa cgcgcgttga tgtagctgct ggtgccgatg ccattatgaa
    12841 agcggtcggt gagtgcccaa aactggaaaa cgtgacggga gaaccaggca ccaacatcgg
    12901 cggaatgctg gaacatgttc gccagaccat ggcggaactg accaacaaac ccagcaacga
    12961 aatttttatt caggatttgc tggcgataga tacctctgtc cccgtcagcg taaccggtgg
    13021 tctggcaggt gaattttcac tggaacaggc tgtgggtatt gcctctatgg tgaaatccga
    13081 ccgtctgcaa atggcgatga tcgccagtga aattaagcaa aaactgcatg ttgatgttca
    13141 ggtcggaggc gcagaagcag aagccgccat tcagggggcc ttgactaccc ctggaaccac
    13201 acgtccactg gcaattctgg acttaggtgc aggctctacc gatgcctcca tcatcaatca
    13261 gagtggggaa atcgttgcca ctcaccttgc gggagcgggc gacatggtaa cgatgattat
    13321 cgctcgtgag ctgggcttaa atgatcgtta tctggcagaa gaaatcaaaa agtacccgtt
    13381 agcaaaagta gaaagcctgt tccacttacg ccatgaagac ggtagcgttc agttcttccc
    13441 tactccccta tccccccatg tatttgcccg cgtctgtgta gtgaaacccg acgaactggt
    13501 gcctattccc ggtgatttga cgctggaaaa agtacgcgct gttcgtcgca gtgcgaaaga
    13561 gcgtgtcttc gtaaccaatg ccctgcgcgc gctacgtcag gtaagccctg ccggaaatat
    13621 tcgcgacatt ccgttcgtcg ttctggtggg tggttcttcg ctggatttcg aagtaccgca
    13681 attggtcacc gacgcgctgg cacattatcg actggtcgcc gggcggggga atatccgtgg
    13741 tagcgaaggt ccgcgcaacg cggtagctac tgggctactc atcgcctggc acaaggagtc
    13801 gattcatgga aagtaaagag cgtgcaccag ccatcgttgt catggagatc ggtgactgta
    13861 tcactacatg ggatgaggtg cttcttggca ttgaagagga aggtattccc tttcgcattc
    13921 aacacattcc gtcaggtgaa gttatcgata gcgcctggct ggctgctcgt caatctcccc
    13981 tgttggttgg cattgcctgc gaccgggaag aactgattgt gcattacaaa aacttaccgg
    14041 catcagcgcc actgtttacg ctgacgtatc aacaagataa ccatacccgg cgcagcatcg
    14101 gcactaacgc tgcccgcctg gtaaaaggaa ttcccttccg ggaattccac tcataatcca
    14161 caggagaaac gctgtatgaa caacgcacta ggactggttg aaacaaaagg gttagttggc
    14221 gcaattgaag ctgcagatgc catggttaaa tccgccaacg tgcagctggt tggctatgaa
    14281 aagattggtt cgggtctgat tacggtgatg gtccgcggtg atgttggcgc agtaaaggcc
    14341 gccgttgatg cggggagcgc tgcggcaagc gccgttggcg aagtgaaatc ctgccatgtc
    14401 atcccgcgcc cacacagtga tgttgaggct attttaccta aatcggcata accccatttg
    14461 ctaaggagca cagcgtgaag caatcactgg gattacttga agccagtggt ctggctctcg
    14521 ccatcagttg cgcagacgct atggcaaaag ccgcgtccat cacgctggtt ggccttgaga
    14581 agaccaatgg ctcaggctgg acgctcatca aaatcaccgg tgacgtcgcc tcggttaatg
    14641 cggcgatcgc gacgggagcc agtttcgccg gacagcgaaa tggccttgtc gcacacaaag
    14701 tgattgcccg cccgggagac ggcattctgc cccacacaac tgaagcattg accgtgacgg
    14761 ttgaaacaag caaaacacca gcaacaaccg tcacctcaga gattatcagt tgtaatttgt
    14821 gtcttgaccc ggcatgtcag cggcaaaaag gtgagccccg ctcactttgc ctgtacgcca
    14881 ataagcgagg ggagtagcag tggataaaca aaccttgcag gaaacagtga gccagattct
    14941 ggatgagatg cgcgagcgcc ctatcccact gggcgtttcc agtcgccata tacatctttc
    15001 atcagcggat tatttgcgtc tttttcctga acaaccgatt agcgagaaaa agccgttaat
    15061 gcaacctggc caatacgctg ctgaacaggt tgttacgctg gagggaccaa aaggcaaatt
    15121 gcacaacgtt cgcttgttgg ggccgctacg caatgcaagc caggtggaaa tttcccggac
    15181 ggacgccaga acgttaggga tcaccgcccc gctacgcatg tcaggcgatc tcagcggcac
    15241 tccggggatt cgtttgataa gcccatttgc ggaactggag atcacatctg gcgtcattgt
    15301 cgcgcaacgg catattcata tgtctccgct ggatgccctg atttatggcg ttacgcatgg
    15361 cgataaagtg gcggttgcca ttgaaggtga tgagcgacga ctgatattcg aaaacgtcgc
    15421 tgttcgcgtc tctccggata tgcgtcttga aatgcatatt gataccgatg aggccaacgc
    15481 cgcaggcgct gatagccctg acgtttttgc cacactggtg tcagtgccat gaataacatg
    15541 ctgatgcaac gtattgttga cgaagtggtt tttcgcctta aacaacgtgc gggaaaaaca
    15601 ctcgtgctga ctgtttttca attgcgagac gccagcgttc aagagagcgt tcatcagtat
    15661 gcgtcattac aaatccgata tgtcgatctg ccgctgctgc gacaacttgc ggagaacgaa
    15721 acgtcagatc gagcatctat ccagatacac gaagcgttag cctggggaat gcacattcaa
    15781 ttgtcgttgc agcgtcattt cctgaacgcc atcgaattga agactctcgc ccgtctaccg
    15841 ctaagctggt gcgacgaaca agggcagccg atctaccttc atcataatcg acttttaagt
    15901 tatgccgata tcgcacagtt aagctcggga atactggtgt tacagcggaa atgttgtgtg
    15961 accgcactgg ctcgtgaagc ggcaattacc cgaaatattc agttaatcag gcaggagtaa
    16021 cctatgcatc tggctcgagt cactggcgcg gtagtatcca cgcaaaaatc accttctctg
    16081 atcgggaaaa aactgctgct ggttcgccgg gtcagtgctg acggcgaact gcctctcacg
    16141 ccattaaccg gcgacgaagt tgccgttgat tcagtcggcg caggcgttgg tgagttggta
    16201 ctcctcagca gtggttccag cgccagacat gtgttttcag gccctaacga ggccatcgac
    16261 ctggcagtgg tcggcattgt cgatacgctt tcgcgctaag gagctaaaag atggcaattt
    16321 atacccgaac aggtgattca ggaagtacgt ctttatttac cggacagcgc gtcagcaaaa
    16381 ctcatctacg tgtcgaaacc tatggcacgc tggatgaact gaacgcgaca ctcagtctgt
    16441 gttattgcgc caccgccatc gaaagccatc gcatcttact ggaagccatt caacaacaga
    16501 tattttggtt tagcgccgaa ctcgccagtg aaagcgaaca gccgtcagcg caacagcgtt
    16561 acatcggtac ggaagaaatt gccgcgttgg aaaacgcgat cgatagtgca atgaacgccg
    16621 tccccccagt tcacagcttc attctgcctg gtcgatgtga agccgcaagc cgcatgcatt
    16681 tcgcccgcac ggtggctcga cgggctgaaa gacggctggt tgaactgaca acggaaacca
    16741 ccgtccggaa tgttttgctg cactatatca accgactttc cgattgcctg tatgccctcg
    16801 cccgcgtgga agacaacgtt gctcatcaaa acctgatgat tcaggaaatc acaaaacgtt
    16861 atcacgaagc caaccacaca ccagcattga aggaacgcac aatgccgctc acttttcagg
    16921 atcttcacca gctcattcgt agtgcggcaa tgcgcgcgga tgaactgcat attcccgttg
    16981 tcatcagcat tgttgatgcc aacggaacag aaagtgttac ctggcggatg cctgatgccc
    17041 tgttggtcag cagtgaactg gcaccgaaaa aagcctggac cgccgtggcg atgaaaacgg
    17101 caacccacaa gcttgctgat accgtgcaac ccgacgcgcc gctttacggg ctcgaaagcc
    17161 atatgcaagg aaaagtcgtc acttttggtg gcggcttccc cctctggcgt gacggaaaat
    17221 tgcttggcgg gcttggcatc agcggcggta gcgttgaaca agacatggat attgcccaaa
    17281 gcgcaatggc ggcaattaac gtgggagtaa accaatgaat aactctgaat tagaaacact
    17341 gatccgcaat attctgcgtg agcaacttgt ccccgctaca ccacgaacgc aaagaaacgc
    17401 tattttccag actgtcgatg aagcggtctg cgcagcacac taggcctttt tacgctttca
    17461 acagtgcccg cttaaaaccc gtagcgccat tatcagcgcc attcgcgaag aattaatgcc
    17521 ccgccttacc gaactggcag aagaaagcgc caaagaaacg gggatgggaa ataaagaaga
    17581 taagtaccta aaaaacaaag cggcactcga taacacgcca ggaattgaag atttaaccac
    17641 cacagcacta accggtgatg gtggcatggt tttgtttgag tactctccgt ttggggtgat
    17701 tggctcggtc acgccaagca ccaaccccac ggaaaccatc attaacaaca gcatcagcat
    17761 gctggcagca ggtaatagcg tctacttcag tccacacccg ggtgccaaaa acgtttcgtt
    17821 aaaactgatt accacaatcg aagacatcgc gtttcgctgt agtggcattc ataacttgat
    17881 cgttaccgta tctgaaccga cctttgctgc caccgaacag atgatggcgc accctaaaat
    17941 cagcgttctg gcgattaccg gcggtccggg gatcgtcgcc atgggaatga aaagtggcaa
    18001 gaaagtgatt ggtgccggag cgggtaaccc tccgtgcatc gtcgatgaaa cggcggatat
    18061 tgtgaaagcc gccgaggaca tcatcaacgg cgcttcattt gattacaacc tgccgtgtat
    18121 tgctgaaaaa agcctgattg tcgtggcatc agtggcagat tatttaattc agcagatgca
    18181 aagttttggc gcacttcttc ttaactatga gcaaactgag aaattgcgtg caatttgcct
    18241 gccagacggt agcgccaaca aaaaactggt tgggaaaagc ccatcagcct tgctggaagc
    18301 ggcaggcctc cctttgcccg caaaagcgcc gcgcctactg attgctgttg tcgacgccga
    18361 tgatagttgg gttacttgtg agcaactaat gccaatgctg cccattgtga aggtcaagga
    18421 ttttgacagc gctctaaccc tggcgctgaa agtggaagac ggcttgcacc acacggcaat
    18481 tatgcactct cagaacgtct cacgactcaa ccttgccgcg cgcgttatgc aaacctcaat
    18541 atttgtcaaa aacggcccct cctgggcggg tattggcgtc ggcggcgaag gctttacgac
    18601 ctttaccatt gcaacaccga ctggagaggg tacaacgtca gcacggactt ttgcccgctc
    18661 acgacgctgc gtgttaacca gcggcttttc aattcgctag agaaacggaa atgaatactt
    18721 tttcactgca gacgcgttta tacagcggac caggaagtct caaggtactt agccgtttct
    18781 ctcatagaca catctggatt atttgcgatg cctttctggc acgctcacca cttattgaca
    18841 cgttgcatca ggcgctgccc gacagtaatc gtctcagtat ttttagcgaa ataacgcccg
    18901 ccccaactat ccagaccgtg gtgaaaggca ttgcgcaaat gcagacttta cgcccggatg
    18961 tagttatcgg ttttggcggc ggttctgccc tggacgccgc aaaagcaatt gtctggttcg
    19021 gtcgccagtg tggcattgaa attgaaacct gcgtagccat tcctaccacc agcggtatcg
    19081 gttccgaagt gaccagtgct tgtgtcatca gcgatcctga gaaagggatt aaatatcccc
    19141 ttttcgataa tgcactttat ccggatatcg cgatccttga tccatctttg gtcgtcagcg
    19201 tgccaccggc gataacggcc aataccggga tggatgtttt aacccatgcg cttgaagctt
    19261 atgtttctcc tcgcgccagc gacttcacgg atgcactggt cgaaaaagcc gtgcaaattg
    19321 tttttcaata cctgcccact gcagtgaaaa aaggcgattg tcttgccacc cgtggcaaaa
    19381 tgcataatgc atcgacgctt gcaggaatgg cctttagcca ggccggactc ggtttaaacc
    19441 atgcaattgc tcatcaactc ggcggacagt ttcatctgcc gcatggactg gcgaatgcgc
    19501 tcctgctcac ggcagttatc cgctttaatg ctggcgatcc gcgcacagcc aaacgctatg
    19561 cccggctggc gaaaacgtgc catttatgcc cggacaacgc caatgacacc gccagcctga
    19621 atgcacttat tcaacatatt gagcagctta atacaacctg tacgctgccg actcttgcca
    19681 atgcgctgaa agaaaaaaaa gcagaatggt caatacgcat accagatatg gttcaggcgg
    19741 cgctggcgga tgcaacgttg cgtaccaacc cgcgtgcagc tgatgcctcc gcgattgcag
    19801 aactgctcga ggagttgtta tgaacgtcgg attaatctcc tgcgacgacc agaccatccg
    19861 cgagcgagta caagcagcag gtgtcgtggg tgctggcggc gcaggttttc caacccatat
    19921 taagctacaa gcgcgtgctg acactttcct ggtgaatgcg gcggaatgcg aacccatgtt
    19981 gaaagtagat caacagttaa tggcgcagca agccagccgc ctgcttcgcg gtgttcacta
    20041 tgcgatgaaa gccaccgggg ccagtagcgg aatcatcgcg ctgaaagaaa aatatcaacg
    20101 ggcaataaac gcactcacac cactgttacc accggatatc cgcctgcata ttttgcccga
    20161 cgtttacccc gcaggagacg aagtgctgac catctggatg gcaactggtc gccgcgttcc
    20221 acccgctgca ttacccgtta gcgtcggtgt ggtggtcaat aatgttcaaa ccgtacttaa
    20281 tattgccaga gccgtcgagc aacagtatcc agtgacgcgc cgcacactca ccgttaatgg
    20341 tgcagtggcg aagccaataa cggtaaccgt ccccatcggc atgtctttac gtgaagtcct
    20401 ggcgctggcg ggcggcgcaa cagtggatgc tcctggtttt atcaatggcg ggccgatgat
    20461 gggaagtttg atcacatcgc tcgacacacc agtcagcaaa accactggcg gcttgctggt
    20521 tttacccaac tctcattcac tgatccaacg tcgaatgcag aatgaccgca gcgtacttgc
    20581 cgtcgcgaaa actgtttgtg agcaatgccg cttgtgtacc gatctctgcc cacgtcattt
    20641 aatcggtcac gaactggcac cccatttatt agtacgtgcg gttaactacc aacagctcgc
    20701 cacgccgcaa ctgttactga cagcgctcac ctgttcagag tgcaacgtct gcgccagtgt
    20761 cgcctgtccg gtaggaattt caccaatgcg cataaaccgc ctgcttaagc aggaattacg
    20821 cgcacaaaat ctccgctacg aaggtgcctt aaatcctgca gatcccatgg caaattatcg
    20881 actgattccc gtaaaacggc tggtaaccag acttggctta accccgtggt atcaggatgc
    20941 gccattgtct gaacaggtgc ctcagcccga aaaagtgacg ttattgttac gccagcacat
    21001 cggtgccagc gcaattgcgt gtgtacaaaa aggcgatcgc gtcgttcatg ggcaatgtgt
    21061 aggtcaaatt ccacacggca cattaggtgc ccccattcat gccagtattg atggcatggt
    21121 cagcgatgtg acagaaaacg cgattacgct tgtgagaggt taaccatgtc gaaagcggta
    21181 ggtattttag aacttagcag tatcgcgaaa gggatggaaa ccggcgacgc catgctgaaa
    21241 agcgccaacg ttgagttact ggtcagcaaa acgctatgcc ccggaaaatt tctgctgatg
    21301 ctcggtggcg atgtgggcgc ggtgcaacag gccatcaaca gcggagcgtc ccttgctggt
    21361 gaaatgctgg tcgacagtct tgtcctgccg aatattcatc ccagcctcct ttctgcaatc
    21421 agcggactta accatgttga acagcaccgg gctgtcggcg tagtcgaaac ctggagtgtc
    21481 gccgcctgca ttgacgctgc cgattgtgct gtcaaagctg caaatgtcat tcttgttcgc
    21541 gtacatatgg cttttggcat tggcggtaaa tgctacatgg tggttgctgg cgatatttca
    21601 gatgttaata acgcggtctc tgccgccagc gtgcgcgcgg gggaaaaagg tttgttggtc
    21661 tatcggtcgg tgattgcccg tccacatgaa gccatgtggc gtcagatggt ggagggataa
    21721 tgacaactca gcacacaact gaacggatga ttcaggaata tgtcccaggt aagcaggtca
    21781 cgctggcaca tgtcatcgcc aatccaggga aggatctgtt taagaaattg ggtttaccag
    21841 acaccatttc cgctattggt attctgacaa ttacccccag cgaagcatct atcatcgcat
    21901 gcgatatcgc cacaaaatca ggcgctgtag agatcggttt tcttgatcgt tttaccggtg
    21961 ccgtagtgct gactggtgat gtttctgcga tcgaatatgc acttagccag gttacccgta
    22021 cgctgggtga aatgatgcgc tttaccgcct gccccattac gcggacctga cgttatgcaa
    22081 cgtatgatgc tcatcggccc cagccagtgc ggaaaaacgt cactcacgca atgtctgcgt
    22141 ggcgaaaata ttcattatca gaaaacgcag gcaatcgtct ggtccccgac caccattgat
    22201 acaccaggcg aatacctcga aaaccgcagc ctctacagtg cactgttggt cagtgcctgc
    22261 gaagcggaca ttatcgcgct ggtgcttaat gcggacgcac cgtggtcacc tttttcgcca
    22321 ggctttacct cgtcaatgaa tcgcccggtc attggagtag taaccaaagc agatttagcc
    22381 aacatggaac aaatatcttt ggtgaaatgt tggttacggg aagctggagc gcacaacgtg
    22441 ttagtaacca gtgcggttaa taaccatgga gttgcagaac ttttagcctt gctgcataca
    22501 gaagaaggct gtcgctaatt tcgacaggtt atcagatggg aatgatgacg gtgatacaca
    22561 gaaaagaatc aatgagcaat gagggttgcc gggcaaccct cattgaataa aacaggaatt
    22621 acttcgcttc gccgtttttc atttcgccca tagctttcag ctttttggag atatcgcggc
    22681 gttctttgga gagctcggca tttttgatga tgtaatcgtc aacgcgatct tcatagtcac
    22741 ctttcatgct ggcgatgatg ccctggattg cttcaacgct catcccaggc ttgatgtaat
    22801 cgctcaggtt gtccagcagc aggacgcgct tctggttgtc acggatcttt ttctcaacgt
    22861 cctgaatttc acgttgcagt ttgttcttac gacggaacag acgaacaaat tccagtacgt
    22921 cctggaatga aggcttggta gtttccattt ttatacccct gataatgtga gagtcagatt
    22981 cgtttaagca accgctattc gttagggcca acattactca tagctgccgc agatgacaat
    23041 gcttttatcc cttttcacta tcataccctt tatccttgct gaatcgaagc agcagcaaga
    23101 tgattctgaa gttcaggaat tatttgcgca aggcccggca aatcagattc gatagcaact
    23161 caagctgatg cgccgtttcc atgttcagcc acacataacc atagattggt gtttccacta
    23221 ccttcaggtc atggtggtta ttgagcaact gacgcagctc ttccacagca tcgttcaatt
    23281 tttccgtatt ggcaaaaacc ggctgcggat taccttcgta cagcgcatga acaaagctca
    23341 gcagtatttg ctgcatcatg tgctgggtat cacgaagttt ttgcgcgttc aataacacga
    23401 aatggctggg gcgcgtggcc cagtacgcat tgatttgcaa ctccagcata caaaccagat
    23461 tgcggttaat ggtctggata ccttcatata tcgattttgg aatacgggtt tctttgctgg
    23521 cgggcgcaat cagcccacgc attttcacgg catcggtcag gagtttttgt agatggcttt
    23581 ccagacgtgg gcgttcgagt aagttcggtg agaatgcaga ttgatagacc cgattatact
    23641 cggtcagact tttcgccagt tgaatgcgcc agtggatgaa cgcccgttgt ggccagatac
    23701 cggtaaacaa cattgccagt aaagagccga ggatcacatc gccgcttcgc cataacgccg
    23761 tatcaatttc acctgtcggg gaaccaacaa caattgccag cgtcacccca atcaataatc
    23821 cttgatacgg tttcttgccc agcgccagcc aaccgcaaag aaacatcgcc gccgcgcacc
    23881 agactaacat cagcggtaac gaaattaact ccagttgcag agcgataaga cctaaaatcg
    23941 aacccaacac cgtaccgcca atacgttcga aggcacgagg gacaacgtta ccccagaacg
    24001 agattggccc cataatcacc accatggtga ccagcggcca ggtgctttcc gggatagtaa
    24061 acaggcggat aatgagaaaa gtgagcagga atgccagcgc gacccgagta ccatgcacta
    24121 tgcggtaatg gcggtatacc cggatttcaa acgggcttaa tgacttatcg gcacgcacgc
    24181 agaaactccg taatgaagat taaaagcgct cataactcat ctgtcggcca gaaggcgcgc
    24241 ctctgaatta tccgacggac aaaccgggaa cagtgtaatt tcccggcagg agcgaaaaaa
    24301 ttgaaaggcg catcttactc ttttcgcttt caaaaaaaga gtggtcatcg cgttaacaca
    24361 ccgccctgag atgaattagt gatgttttgg ctgcaccgca acatacattt cgatatccca
    24421 gtacccatct tccgcgccat tgttcaaata aacctcgaag cagggctttg gtaacatttc
    24481 ataagcactg tcctgcaaga ggctattaaa gaactgatac cagggtttag caaaatcatc
    24541 accgactaca cgagccaccg ccaccgcata ctgaccacct gaaatttctg tcagaatgac
    24601 gccctcactg ttttcgggaa gcgtaaagtt attcggtacc gtcacaacgg tgtcgcagcg
    24661 taatttttcg gcgggtgttt catctggatt gtcgtaatag acagcaaccc actccttcgg
    24721 cacaatattt ttgttatcta cccacatcat caactgctca aagcctttct ttaccgtctg
    24781 ttcccacggg ccaacgagat ggaaacctgc aacggtacgt ttctcttcct gcgtaatctc
    24841 gtagttcatg acgcctccat taacactgtt tttatataca gcatagttgc aatattaaaa
    24901 ccacaaggaa tgagtgttga ttatgcgagc agactcgcac tcctgccagt ctgctgcaaa
    24961 agaaaggtca ggccttatgg tggaaataat cactcaggcg ggaaaacatg ccgccttccc
    25021 cgacagattc cagggtaacc agcggccagt gcgccacctg tttatcacgg tcgtaaagtt
    25081 caatttcccc tacccgctga tgggcgctaa ttggcgcggt gagctcttta ccatcaaggg
    25141 tatatttggc tttgatatgt ggaatttcgg ctttcggtag caccatccag aactcttgtt
    25201 ccgttcctag gtcgatattt tctttatcgc cataccagat gcgttccgtt ccgacctttt
    25261 tcccacggtg caaaatttgc accgtagtaa agttttgttg cccccaacgc agtaattttc
    25321 ttgcctcttc ctcacgacct tttgcactgt cagcccccat taccactgca atgagacgac
    25381 gctgcccatc tacagccgaa gcaatgagat taaacccggc accagaagta tgacccgttt
    25441 tcaggccgtc aacattcatg gttttatccc acaacaaccc gttacggttt tgctgggtga
    25501 taccgttcca ggtgagactt ttctcactgt acatatgata aaactcgggc tcgccgtgga
    25561 tgatagcgcg agaaagcaca gctaaatcat aagccgagct atgctggcca ggtgcatcca
    25621 gaccatgcac tgtttcaaaa tgcgtatcct tgagatgcag cttctcggca tagttgttca
    25681 tcatttcaac aaactgccgt tgcccaccgg caatatagtc agccagagca acacaagcgt
    25741 catttccgga atccacaatt aaaccacggc ttaaatcacg taccgatacg cgatcgccct
    25801 ctttcaaaaa catcagtgaa gaaccgacaa acaccggatt atctttcgcc cacgcatcgc
    25861 gccccacggt gacaatatcg tctggcgtaa tgcgatgact atcgatagcg cgatccacga
    25921 cataacccgt catcagcttt gtcaggctgg cgggattgcg ctgttgatgc tcattacccg
    25981 cggtgaggat ctgaccggtg gtgtaatcca tcaataccca ggacccggca tgaatctctg
    26041 gaggctgagg tgaaaaagga atgttttccg ccgcaaaacc agacgataag ttaaaaacga
    26101 acaaagaagc agcaataata agacggcgtt tcaacagcaa accctcagga gtttcaaata
    26161 gctgttcttt ttacggaaat acttatgaac tggctggaat aaagtgcaag aaaatgtgac
    26221 taccctctca tttttatctg acatgatctg ttgccactcg ctgccaaatt gtggcgctaa
    26281 agctgattag cacggtgata tttgatactc tggcagacag cagaaataac ggatttaacc
    26341 taatgatgaa tgacggtaag caacaatcta cctttttgtt tcacgattac gaaacctttg
    26401 gcacgcaccc cgcgttagat cgccctgcac agttcgcagc cattcgcacc gatagcgaat
    26461 tcaatgtcat cggcgaaccc gaagtctttt actgcaagcc cgctgatgac tatttacccc
    26521 agccaggagc cgtattaatt accggtatta ccccgcagga agcacgggcg aaaggagaaa
    26581 acgaagccgc gtttgccgcc cgtattcact cgctttttac cgtaccgaag acctgtattc
    26641 tgggctacaa caatgtgcgt ttcgacgacg aagtcacacg caacgttttt tatcgtaatt
    26701 tctacgatcc ttacgcctgg agctggcagc atgataactc gcgctgggat ttgctggatg
    26761 ttatgcgtgc ctgttatgcc ctgcgcccgg aaggaataaa ctggcctgaa aatgatgacg
    26821 gtctaccgag ctttcgcctt gagcatttaa ccaaagcgaa tggaattgaa catagcaacg
    26881 cccacgatgc gatggctgat gtgtacgcca ctattgcgat ggcgaaactg gtaaaaacgc
    26941 gtcagccacg tctgtttgat tatctcttta cccatcgtaa taaacacaaa ctgatggcgt
    27001 tgattgatgt tccgcagatg aaacccctgg ttcacgtttc cggaatgttt ggggcatggc
    27061 gcggcaatac cagctgggtg gcaccgctgg cgtggcatcc agaaaatcgc aatgccgtaa
    27121 ttatggtgga tttggcagga gacatttcgc cattactgga actggatagc gacacattgc
    27181 gcgagcgttt atatactgca aaagccgatc ttggcgataa cgccgccgtt ccggttaagc
    27241 tggtgcatat caataaatgt ccggtgctgg cccaggcgaa tacgctacgc ccggaagatg
    27301 ccgaccgact gggaattaat cgtcagcatt gcctcgataa cctgaaaatt ctgcgtgaaa
    27361 atccgcaagt gcgcgaaaaa gtggtggcga tattcgcgga agccgaaccg tttacgcctt
    27421 cagataacgt ggatgcacag ctttataacg gctttttcag tgacgcagat cgtgcagcaa
    27481 tgaaaattgt gctggaaacc gagccgcgta atttaccggc actggatatc acttttgtcg
    27541 ataaacggat tgaaaagctg ttgttcaatt atcgggcacg caacttcccg gggacgctgg
    27601 attatgccga gcagcaacgc tggctggagc accgccgcca ggtcttcacg cccgagtttt
    27661 tacaaggtta tgcagatgaa ttgcagatgc tggcacaaca atatgccgat aacaaagaga
    27721 aagtggcgct gttaaaagcg ctttggcagt acgcggaaga gatcgtctaa tttgaagcct
    27781 ccgccgctgg tacggcggag gtcaatgttg agtcaggctt tttgaacggt gatactccac
    27841 gctgcatcgc caatctgctg ataatcggtg atggcatgcc cttcttctgc cgcccactgt
    27901 gggattgctt cagttgcctg ggtgcaatca aactcaatta ccaactcatc gccgctggcc
    27961 atttctgcca gtgctgcttt tgcttctatg agtggaaacg gacagacctg cgtcaccaca
    28021 tccagctttt taatcgccat ctttacacct tacttaattt gccgcagcag ttgccagtcg
    28081 cgccttacgc tgcggtcgaa catacacaag ccaggacgca gtccagactc cgagaatcat
    28141 aaatacaagg ccaatccagc cctgccaggt catcattgca gtcataacca gtccattacc
    28201 gataaagcaa ccacctgcaa tactggcacc gaagcccatc agtacaccac ctaacccgct
    28261 acgtaatgtt gtttgtgcat cagctgcgcg aacgcggaac tcacggctcg ctttggctgc
    28321 aataaatgac cccacgaaga tccctaacac caggaaaacg ccccagttaa tgtatttcac
    28381 gtcacccgcg accagaaatt gcagaatatt ggccgttggg gaagtgattc ctaacccaaa
    28441 catgcgtccg gttgcttcac tcaggggcca ggctaaaagc gcaatcaaac cgatgagtac
    28501 agcagtgaca aagggatgcc agcgtttttc aaacagaata tgagcgatcc cagttcggcg
    28561 cggcggtaag gtcgcgactt tgagttttgg cttcttcaac tctttcatca ccacccagag
    28621 cgttatcacc agcaaaacgg caaccaacgg ccaaacagac aaattaaaag tctcagcaat
    28681 agagttatgt tcagtactgt agtgctgcaa ggtttgattt aaaccactgg catgtggaga
    28741 acgcatcacc gcactcatca ccatataagt gaaaagcgcg atccaactgc cgatcaatcc
    28801 ctcacccgcg cgataccagg tcccggtggc acatccaccc gccagaacaa ttcccagccc
    28861 gaagatatac ccacctataa cagtaccaag ccacgggaac gcctcggctt cgtaagtcag
    28921 taggcccgcc tgaattaacg caaaaacccc cacgctttgt accgaaattg caatcagcaa
    28981 ggcgtaaaac atgcgattat ttttcgcgat atacatatcg cgaaaaccac ctgtcaggca
    29041 aaaacgccca cgctgcatga caaatcccag cagagcacca caaattagcc cgcttaatat
    29101 cattgaaaac ataaattaat taaccagatg aatgttaatg aggaaattat tcatgactgg
    29161 tggaatgcag accaataacc aaattctcta aattagaaca aatggttatt aatgaggatt
    29221 tcgataaaaa aaaataaaaa aaccggagcc aaatattcat tcgactccgg ttgattatta
    29281 gaaaagatga ttatgcaacg tcttcgtact gcggaactgg gttacggaag ctcttggtga
    29341 cgcaagccag gtaaatcaga ccgatagcag cccaaatcag acccagaacc attgagcttt
    29401 cttccaggtt aacccacagc gcaccaacgg tcagcgcacc acacatcggc aggaacagat
    29461 actggaagtg atctttcagc gtcttattac gcttctcacg gatccagaac tgcgagatga
    29521 ccgacaggtt aacgaaggtg aacgccacca acgcaccaaa gttaatcagc gctgtagcca
    29581 ttaccaggtc gaagttgatt gccagcaggg caatcgcgcc aaccaggatg atgttcatcg
    29641 ccggagtacg ccatttcggg tgtacataac cgaagaagct tttcgggaat acgccgtcac
    29701 gacccattac gtacatcaga cgcgctacgc ctgcatgcgc cgccataccg gacgccagta
    29761 cggtaatggt ggagaagatc agcgcgccaa cctggaacgc tttacccgca acgtacagca
    29821 tgatttcagg ctgtgacgca tccggatctt taaagcgaga gatatccggg aagtacagct
    29881 gcaggaagta agttgcaaag atgaagatca tgccgccaat cagcgcggtc aggaaaatcg
    29941 cacgcgggat cacgcgctct gcatctttgg tttcttccga caggttgctg atgccgtcaa
    30001 agccggtaaa ggaaaagcac aggattgtcg ccccggtaat catcggaata acatgcgcat
    30061 cgccagacca gaacggacga gtgctcgcca gcgtaccagc gccttcacct tcaaatacgc
    30121 cataaacaac catgcccaga atcaccgcga tcagcactac ctgcaacacg acgattacag
    30181 tgttgaagtt cgctacggat ttcagactac gcaggttaaa ggcggtcata aaggccacca
    30241 gcgccaccac aaacatccac gatgggatgg aaggcaccag agcttcaaaa tagattttcg
    30301 ccagcagaat gttgatcatc ggcgcgaaca gatagtcgag cagagaagac caacccacca
    30361 taaagccgac agtcgggcta atggatttct gggcgtaagt atatgcagag ccagcagaag
    30421 gatagcggcg aaccagcttc ccgtagctca gagccgtaaa caggatcgca atcaatgcga
    30481 acgcataggc tgtcggcaca tgaccatccg tgaggcctga aacgatacca aaagtatcaa
    30541 acagcgtcat cggctgcata taggcaagac ccatcattac aaccggaact aacgtaagcg
    30601 ttttacgcaa ttccacgcga gaggtgtttg gagtaacgtt atgcgacatg gtcattctcc
    30661 tttacggtga taaccgtcgc gtaagcaaaa aattgcccca tttttttgga ttcctcagcg
    30721 acaacaactg tcgattttta gtaaatatct atccggtacg aagcccggcc tcttggtatg
    30781 aatgattggt ttgaagcaaa aaataaccga cgctgatgaa acgtcggttt ttagtcattt
    30841 tttgacagcg gcgcattgtg cctaaatggg ggggaaatga caagagaatg agaagcttgt
    30901 cataataatt tttctttaaa tggctgattt tccgtcatca gattttgcat aaacaccggc
    30961 gatcgcacta tttgctaaaa tttcatcccg ccaccagccg ctggcaagcc ctgcggtctg
    31021 ttcgttccag ccaatccaga cgggttcaaa gagagtttgg gcgaccactt tcttctcgat
    31081 taacgcgcca ctatcgagaa aacgttgcac cagataacgg ggtaaatagc cgcagccgag
    31141 gccgctaatt tgcagctcca gcttggtttt aaaatcgaaa acggtaatgg cttcttgttc
    31201 gtcgagaagt tgtgaggccg tagaagcgcc tgcctgcgca gtgtccccca ccacaatcgc
    31261 ccgatatcgc ttaatgatac gacggtttaa cggctcttct tcttgggcta acggatgatg
    31321 aggcgcgacc gcaaagactt gttccagatc gcccaaccgc gagaaaccaa actcactgga
    31381 agatggtggc tcatgcatcg cgccaacaat aatatccgct cgcccctgag tcagggcatc
    31441 ccaggaacca ccgagtacgc cattgataaa cttcaggcgc gtcacgctat gatgttgata
    31501 aaaagcttca atcagtggcg caagcagaga aaaaggaaac gtgtcgtcca cgccaatcac
    31561 cagctcgttt tcccagcctt catgcagttt gatcgcctgc ttttccagtt ctcgcacggt
    31621 atgcagaact tcccgcccct tctctaataa catttttccg gtacgggtga atttagcgcg
    31681 gtgaccgcta cgatcaagta attgaatatt aaggtcgctt tccagcttgt gaacggtgta
    31741 actcagagcg gacggcgtct tgtagagttt agccgacgcc gccgcaaaac tgccttcttt
    31801 ttctaaggca tcgaggatca tcaacacatc cagcagttgt ttcatacacg cccccttgca
    31861 gtgcatgcgg cgtcctgctg gcgtgatcgt ttactccagc ggcattacca gcggatcggg
    31921 atactggtat tcaaatccca gttcattaca aatccgactg ccatcaataa tcttaccttt
    31981 gccgctgtcc agactatttc tgaactgcgg aggttccagc cccagtaaac gggccatctg
    32041 cggatagaaa acattacgcg cagggtgagc tggcgcacat atattataga tgtgtccgcc
    32101 tttaggtgcc tgtaacaaca gagtgatagc gccaataaca tcttctaaat ggactaaatt
    32161 aacaccatgt tcaccatcag gcgcggtttt tccggcaaag aagcgtccgg gatgacgtcc
    32221 cggtccgacc agccccgcaa gacgcagaat atcgaccgaa gtaccgggta aattgtgcag
    32281 ccagtcttcg agttcttcta atactcgtcc actgttggtc accggattac gcggggtggt
    32341 ttctttcacc gtgccttgcg cgtcgccata gacagacgtt gagctggtaa aaataatgcg
    32401 aggaatacga tgggccagcg cgctatccac taactcttgt accgcttgta aatagaactc
    32461 atcgccgggg ccgctacgac gtgccggaag cgtaatgacc agcgcatcgg catccatcag
    32521 ggcatccaga tcgtcagaat cgcaaactaa ctcaggctcc atgcgaagca gatagctatc
    32581 aatgccactc attcgggccg cttcgacacc atcttgtgtg gttttactcc cggtgacttg
    32641 ccagcctcgc gctgaaagtg acatcgccag cggcatgcct aaccacccta acccgacaat
    32701 tgcgaccttt ttcatccgtt atctcctgat acaccttaga tctataaggc tacgctagcg
    32761 tatcaaaact gacaattcat tctatgaatg aatctgttca ataatgataa cgacatgctg
    32821 caatgagcag tgaatcgtcg gtaaccgcgt ataccagacg gtgctcctct gtaatgcgtc
    32881 gggaccagaa acctgacaaa ttatgtttca ggggttctgg cttcccctta ccttcaaatg
    32941 gcgttctgcg ggtatctttg ataagttcat tgatcttttt aacaattcgc ttatctgttt
    33001 cctgccagta cagataatca tcccatgatt cctcagacca gattagtttc actcaataat
    33061 gtccttttct gttcctttgc ctgatttcag gctatcgatt gagtccatca atctccgggc
    33121 gttagcgggg gagcgcagta gataagccgt ctcttccagc gagttgtatt cttcgagtga
    33181 catcagaaca caagcctctc cattctgacg agtaataagg atcggggcat gatcttcaac
    33241 ggctttcatc attgttgccg acaaattctg acgcgcttcg ctgtagctaa ttgtacgcat
    33301 gtcaatctcc tcttttgtac agtttattgt acaatgatgg gcgttaatta actatttatt
    33361 aattagtttg tagatcaagg tattgtcagt gagacgaaaa tccaggcttc gctatttttg
    33421 gtgccgtcag cttagaggac agtcctctta gccccctcct ttccccgctc attcattaaa
    33481 caaatccatt gccataaaat atataaaaaa gcccttgctt tctaacgtga aagtggttta
    33541 ggttaaaaga catcagttga ataaacattc acagagactt ttatgacacg cgttcaattt
    33601 aaacaccacc atcatcacca tcatcctgac tagtctttca ggcgatgtgt gctggaagac
    33661 attcagatct tccagtggtg catgaacgca tgagaaagcc cccggaagat caccttccgg
    33721 gggctttttt attgcgcggt tgataacggt tcagacaggt ttaaagagga ataacaaaat
    33781 gacagacaac actcgtttac gcatagctat gcagaaatcc ggccgtttaa gtgatgactc
    33841 acgcgaatta ctggcgcgct gtggcattaa aattaatctt cacacccagc gcctgatcgc
    33901 gatggcagaa aacatgccga ttgatattct gcgcgtgcgt gacgacgaca ttcccggtct
    33961 ggtaatggat ggcgtggtag accttgggat tatcggcgaa aacgtgctgg aagaagagct
    34021 gcttaaccgc cgcgcccagg gtgaagatcc gcgctacttt accttgcgtc gtctggattt
    34081 cggcggctgc cgcctttcgc tggcaacgcc ggttgatgaa gcctgggacg gcccgctctc
    34141 cttaaacggt aaacgtatcg ccacctctta tcctcacctg ctcaagcgtt atctcgacca
    34201 gaaaggcatc tcttttaaat cctgcttact gaacggttct gttgaagtcg ccccgcgtgc
    34261 cggactggcg gatgcgattt gcgatctggt ttccaccggt gccacgctgg aagcaaacgg
    34321 cctgcgcgaa gtcgaagtta tctaccgctc gaaagcctgc cttatccagc gcgatggtga
    34381 aatggaagaa tccaaacagc agctgatcga caaactgctg acccgtattc agggtgtgat
    34441 ccaggcgcgc gaatcaaaat acatcatgat gcacgcaccg accgaacgtc tggatgaagt
    34501 catcgccctg ctgccaggtg ccgaacgccc aaccattctg ccgctggcgg gtgaccaaca
    34561 acgcgtagcg atgcacatgg tcagtagcga aaccctgttc tgggaaacga tggaaaaact
    34621 gaaagcgctg ggtgccagtt caattctggt cctgccgatt gagaagatga tggagtgatc
    34681 gccatgagct ttaacacaat cattgactgg aatagctgta ctgcggagca acaacgccag
    34741 ctgttaatgc gcccggcgat ctccgcttct gaaagcatta cccgcactgt taacgatatt
    34801 ctcgataacg tgaaagcacg cggtgatgac gccctgcggg aatacagcgc gaagtttgat
    34861 aaaaccacgg ttaacgcgct gaaggtgtct gctgaggaga ttgccgccgc tagcgaacgc
    34921 ctgagcgacg agctaaaaca ggcgatggcg gtggcagtaa aaaatattga aaccttccac
    34981 actgcgcaaa aactgccgcc ggtagatgta gaaacgcagc caggcgtgcg ttgccagcag
    35041 gtcacgcgcc cggtcgcttc agttgggttg tatattcctg gcggctccgc cccgctcttc
    35101 tcaacggtat taatgctggc gactccggcg cgtattgcgg gctgtaaaaa agtggtgctg
    35161 tgctcaccgc cgccgattgc cgatgagatc ctttatgcgg cgcagctgtg cggtgtgaag
    35221 gacgtgttta acgtcggcgg cgcacaggcc attgccgcgc tggcgtttgg tacggaatct
    35281 gtgccgaaag tggacaaaat cttcgggccg ggtaacgcct ttgtcaccga agcgaaacgt
    35341 caggtgagcc agcgtctgga cggtgcggct atcgatatgc ccgcaggccc gtcggaagtg
    35401 ttggtcattg ccgacagcgg cgcaacgccg gatttcgtgg cttctgattt gctttctcag
    35461 gctgaacacg gcccggactc acaggtgatt ttactgacgc ctgacgctga tatggcgcat
    35521 caagttgccg aagccgtcga acgccagtta gcagaactgc cgcgtgccga aaccgcccgc
    35581 caggcactga acgccagccg cctgatcgtg actaaagatt tagcgcagtg cgtagagatc
    35641 tccaaccagt acggcccgga gcacctgatc atccagaccc gcaacgcccg cgatctggtc
    35701 gatggcatca ccagcgccgg ttcggtattt cttggtgact ggtcaccgga atcggcaggt
    35761 gattacgcct ccggcaccaa ccacgttctg ccgacttacg gttacaccgc cacctgttcc
    35821 agcctcgggc tggcagattt ccagaagcgt atgaccgtgc aggaactgtc gaaagaaggt
    35881 ttctccgcgc tggcttcgac cattgaaaca ttggccgccg ccgagcgcct gaccgcccat
    35941 aaaaatgccg ttactttgcg tgttaacgcc cttaaggagc aagcatgagc accgtgacta
    36001 ttaccgattt agcgcgtgaa aacgtccgca acctgacgcc gtatcagtcg gcgcgtcgtc
    36061 tgggcggtaa tggcgacgtc tggctgaacg ccaacgaata ccccactgcc gtggagtttc
    36121 agcttactca gcaaacgctc aaccgctacc cggaatgtca gccgaaagcg gtgattgaaa
    36181 attacgcgca atatgcaggc gtaaaaccgg aacaggtgct ggtcagtcgt ggcgcggacg
    36241 aaggtattga actgctgatt cgcgcttttt gcgaaccggg taaagacgcc atcctctact
    36301 gcccgccaac gtacggcatg tacagcgtca gcgccgaaac gattggcgtc gagtgccgca
    36361 cagtgccgac gcaggacaac tggcaactgg acttacaggg catttccgac aagctggacg
    36421 gcgtaaaagt ggtctatgtt tgcagcccca acaacccgac agggcaactg atcaatccgc
    36481 aggattttcg cactctgctg gagctaaccc gcggaaaagc cattgtggtt gccgatgaag
    36541 cctatatcga gttttgcccg caggcatcgc tggctggctg gctgacggaa tatccgcacc
    36601 tggctatttt gcgcacactg tcgaaagctt ttgctctggc gggccttcgt tgcggattta
    36661 cgctggcaaa cgaagaagtc atcaacctgc tgatgaaagt gatcgccccc tacccgctct
    36721 cgacgccggt tgccgacatt gcggcccagg cgttaagccc gcagggaatc gtcgctatgc
    36781 gcgaacgagt gacgcaaatt attgcagaac gcgaatacct gattgccgca ctgaaagaga
    36841 tcccctgcgt agagcaggtt ttcgactccg caaccaacta cattctggcg cgctttaaag
    36901 cctccagcgc agtgtttaaa tctttgtggg atcagggcat tatcttacgt gatcagaata
    36961 aacaaccctc tttaagcggc tgcctgcgaa ttaccgtcgg aacccgtgaa gaaagccagc
    37021 gcgtcattga cgccttacgt gcggagcaag tttgatgagt cagaagtatc tttttatcga
    37081 tcgcgatgga accctgatta gcgaaccgcc gagtgatttt caggtggacc gttttgacaa
    37141 actcgccttt gaaccaggcg tgatcccgga actgctgaag ctgcaaaaag cgggctacaa
    37201 actggtaatg atcaccaatc aggatggtct gggaacacaa agtttcccgc aggcggattt
    37261 cgatggcccg cacaacctga tgatgcagat cttcacctcg caaggcgtac agtttgatga
    37321 agtgctgatt tgtccgcacc tgcccgccga tgagtgcgac tgccgtaagc cgaaagtaaa
    37381 actggtggaa cgttatctgg ctgagcaagc gatggatcgc gctaacagtt atgtgattgg
    37441 cgatcgcgcg accgacattc aactggcgga aaacatgggc attaatggtt tacgctacga
    37501 ccgcgaaatc ctgaactggc caatgattgg cgagcagctc actaaacgag accgttacgc
    37561 tcacgtagtg cgcaacacca aagagacaca gattgacgtt caggtgtggc tggatcgcga
    37621 aggtggcagc aagattaaca ccggtgttgg cttctttgat cacatgctgg atcagatcgc
    37681 cacccacggt ggtttccgta tggaaatcaa cgtcaaaggc gacctctata tcgacgatca
    37741 ccacaccgtc gaagataccg gcctggcgct gggcgaagcg ttaaaaatgg ctcttggcga
    37801 caaacgcggt atttgccgct ttggttttgt gctaccgatg gacgaatgcc ttgcccgctg
    37861 cgcgctggat atctctggtc gcccgcacct ggaatacaaa gccgagttta cctaccagcg
    37921 cgtgggcgat ctcagcaccg agatgatcga gcacttcttc cgttcgctct catacaccat
    37981 gggcgtgacg ctacacctga aaaccaaagg taaaaacgat caccaccgtg tagagagcct
    38041 gttcaaagcc tttggtcgca ccctgcgcca agccatccgc gtggaaggcg acaccctgcc
    38101 ctcgtcgaaa ggagtgctgt aatgaacgtg gtgatccttg ataccggctg cgccaatctg
    38161 aactcggtga agtcagccat tgcgcgccac ggttatgaac caaaagtcag ccgtgacccg
    38221 gacgtggtgt tgctggccga taaactattt ttacccggcg ttggcactgc gcaagcagcg
    38281 atggatcagg tgcgtgagcg cgagctgttt gatctcatca aagcctgtac ccaaccggta
    38341 ctgggcatct gcttagggat gcaactgctg ggtcggcgca gcgaagagag caacggcgtc
    38401 gacttgctgg gcatcattga cgaagacgtg ccgaaaatga ccgactttgg tctgccgctg
    38461 ccgcatatgg gctggaatcg cgtttacccg caggcaggca accgcctgtt tcaggggatt
    38521 gaagacggcg cgtactttta ctttgttcac agttacgcaa tgccggtcaa tccgtggacc
    38581 attgcccaat gtaattacgg cgaaccgttc accgcggcgg tacaaaaaga taacttctac
    38641 ggcgtgcagt tccacccgga gcgttctggt gccgctggcg ctaagttgct gaaaaacttc
    38701 ctggagatgt gatgattatt ccggcattag atttaatcga cggcaccgtg gtgcgtctcc
    38761 atcagggcga ttacggcaaa caacgcgatt acggtaacga cccgctgccg cgcttacagg
    38821 attacgccgc gcagggtgcc gaagtgctgc atctggtgga tctgaccggg gcaaaagatc
    38881 cggcaaaacg tcaaatcccg ctgattaaaa ccctggtcgc gggcgttaac gttccggtgc
    38941 aggttggcgg cggtgtgcgt agcgaagaag acgtggcggc gttactggaa gcgggcgttg
    39001 cgcgcgtggt ggttggctcc accgcggtga aatcaccaga aagggtgaaa ggctggtttg
    39061 aacgtttcgg tgccgatgcc ttagtgctgg cgctggatgt ccgtattgac gagcaaggca
    39121 acaagcaggt ggcggtcagc ggctggcaag agaactcggg cgtttcactg gaacaactgg
    39181 tggaaaccta tctgcctgtc ggcctgaaac atgtgctgtg taccgatatc tcgcgcgacg
    39241 gcacgctggc aggctctaac gtctctttat atgaagaagt gtgcgccaga tacccgcagg
    39301 tggcatttca gtcctccggc ggtattggcg acattaacga tgttgctgcc ctgcgaggca
    39361 ctggcgtgcg cggcgtaata gttggtcgtg cattactgga aggtaaattc accgtgaagg
    39421 aggccatcgc atgctggcaa aacgcataat cccatgtctc gacgttcgtg atggtcaggt
    39481 ggtgaaaggc gtacagtttc gcaaccatga aattattggc gatattgtac cgctggcaaa
    39541 acgctacgcc gaagaaggtg cagacgaact ggtgttctac gatatcaccg cttccagcga
    39601 tggccgtgtg gtagataaaa gctgggtatc tcgcgtggcg gaagtgatcg acattccgtt
    39661 ctgcgtggcg ggtgggatta agtctctgga agatgccgcg aaaattcttt cctttggcgc
    39721 ggataaaatt tccatcaact cccctgcgct ggcagaccca accttaatta ctcgcctggc
    39781 ggatcgcttt ggcgtgcaat gcattgtggt cggcattgat acctggtacg acggcgaaac
    39841 cggtaaatat catgtgaatc aatataccgg cgatgaaagc cgcacccgcg tcactcagtg
    39901 ggaaacactc gactgggtac aggaagtgca aaaacgtgga gccggagaaa tcgtcctcaa
    39961 catgatgaat caggacggcg tgcgtaacgg ttacgatctc gaacaactga aaaaagtgcg
    40021 tgaagtttgc cacgtcccgc taattgcctc cggtggcgcg ggcaccatgg aacacttcct
    40081 cgaagccttc cgcgatgccg acgttgacgg cgcgctggct gcttccgtat tccacaaaca
    40141 aataatcaat attggtgaat taaaagcgta cctggcaaca cagggcgtgg agatcaggat
    40201 atgttaacag aacaacaacg ccgcgaactg gactgggaaa aaaccgacgg actaatgccg
    40261 gtaattgtgc aacacgcggt atccggcgaa gtgttaatgc tgggctatat gaacccggaa
    40321 gccttagaca aaaccatcga aagcggcaaa gtcaccttct tctcgcgcac taaacagcga
    40381 ctgtggacca aaggcgaaac gtcgggcaat ttcctcaacg tagtaaatat tgcgccggac
    40441 tgcgacaacg acacgctact ggtactggcg aatcctatcg gaccaacctg ccacaaaggc
    40501 accagcagct gcttcggcga caccgctcac caatggctgt tcctgtatca actggaacaa
    40561 ctgctcgccg aacgcaaatc tgccgacccg gaaacctcct ataccgccaa actgtatgcc
    40621 agcggcacca aacgcattgc gcagaaagtg ggcgaagaag gcgtagaaac cgcactggca
    40681 gcgacggtac atgaccgctt tgagctgacc aacgaggcat cggacttgat gtatcacctg
    40741 ctggtgctgt tgcaggatca ggatctggat ttaacgacgg taattgagaa cctgcgtaaa
    40801 cggcatcagt gagttgcagg gtaagcggat gcgatgttgt tgtcgcatcc ggcaaaaaaa
    40861 aagggcaagg tgtcaccacc ctgcccgttt tctttaaagc tgcaaagatt acttcgcgtt
    40921 ataattacgc agagcgttac gccccagcac aatccccgcg ccaaccatgc cgcccagcag
    40981 caccgccaga atcaaggtaa ttgccttttt cgggctatcg cgacgaatag gtaacgtcgg
    41041 tttcatcaca taacggtaag catgaatatc aagatcatca acctttaagt tttcaatatc
    41101 caacaggttt tgacgtgtct gatagtagtt tggtgagaac accaacggac gggtcgcctc
    41161 atgcttaatc atcgactcca gcgcttcgct ccccaaaagg aacaacgtat cttgtgtcac
    41221 atcttcagtc tgctgaacct gtggctttgc cacctgcgcc tgattcgcat actgcaacgc
    41281 ttcctgaatc tgacggatac gcagatcttt ctgctcctgc gcgactactt cctgcgttct
    41341 taaagagtcc tgcaagtttt tccgtcccag agcaatgttg tctttgagat ccttttctag
    41401 ctcttgattc actttatcat caacttgctg aatgtattgg gccaacttca tttgtgctcc
    41461 ctctgcagtt tgcccaacat aagaaacagt caatggtaat tgctggttct taacagaagg
    41521 ttcgatggta agtttttctg gttcttcctg attatccagc gtttctgcta atgcagagaa
    41581 ggcagaactg aagcgaccaa ttaacgtctc ctgtaaatcc gatactttcg gtgcagcctg
    41641 accatagata acattcatgg cattgttata gccagcaatt tgccccacgt cgggctgagt
    41701 gataattgct gttgacgtcc atttctcctt cgctactgcc aaatatccaa tagccagggc
    41761 aatagccaca atgacggaaa tgatgattgt catcttgcca cgccacaact gcactagtaa
    41821 atcaatcaaa tcaatctgtt ccgggtcatg gttttgccca gaaacattat tattttctac
    41881 tctcatcatt accctaactg acggaaattt ccaagtccgc atagtttagc gataatccta
    41941 gtgaatgcta taggataagt gataaaaata atgaattatt ggggaggtaa caaaagacaa
    42001 tcagggcgta aatagccctg ataacaagat gttagtcgct gccaaagaga tcgcgggtgt
    42061 ataccttatc tgccacatct ttaagctctt ctgccattcg gttagagata atgacgtcgg
    42121 cttgttgttt gaagatggcg agatcacgtt ccagccgaga gttgaagaat gagtcttctt
    42181 tcatcactgg ctcgtagatg atcacttcaa cgcctttcgc cttgatacgc ttcataatcc
    42241 cctgaataga agacgcacgg aagttatctg aaccgctctt cataatcaga cgataaatac
    42301 ccaccacttg cggtttgcgt gacaaaatgg catcggcaat aaaatcctta cgcgtgcggt
    42361 ttgcatcgac aattgccgag atcaggttat tcggcacaga ctgatagttc gccagtaact
    42421 gcttggtatc tttcggcaag caataaccac cataaccaaa cgacgggttg ttgtaatggt
    42481 tgccaatacg cggatcgaga caaacgcctt cgataatctg gcgagtattc agacctaaac
    42541 tttctgcgta gctatccagt tcgttaaagt atgccacgcg catcgctagg taggtgtttg
    42601 caaaaagttt aatcgcttct gcttcggtcg aatcggtgaa cagggtcggg atattttgct
    42661 taatcgcgcc ttcctgtaac aacgcagcaa aacgttctgc gcgttctgaa cgctcaccga
    42721 tgacaatacg tgaaggatgg agattatcgt aaagggcttt accttcacgg agaaattccg
    42781 gagagaaaat aatattttca gtacgatatt tcttatgcat cgcttcggta aaaccaacgg
    42841 gaaccgttga tttgatgacc ataaccgcat aaggatttat ctcaactacg tctttaatta
    42901 ctgattctac actggatgta ttgaaataat tagttttagg atcatagtcg gttggagtgg
    42961 cgatgatgac ataatcagca tcccggtagg cttcattttt atctaatgtg gcattaaagt
    43021 gtattttatc tgattgcaaa aactgctgaa tttccttatc aacaatagga gatatccgat
    43081 cattcagcat agcaacgcgt gacggtaaaa tatctaatgc cacaacctca tgattttgtg
    43141 cgattagaag cccgtttgac aagcctacat agccagtacc ggaaatggtg attttcattt
    43201 catgctctca gaattaactt aactgtgaat catgatgttt ttagcatcct gataagagct
    43261 aaatgtttta acgctacgtt attagctggt caacgctacg aaaaacaatt cagattttct
    43321 ttataaggat agctgatatt tattaagtta atattaagca gtgaaattta gtctataaga
    43381 taaatggcaa aaaaaaagcc cggtgcaata tacgccgggc ctcaatttta ctgttggtta
    43441 aatcagatta atccagccat tcagtatgga acacaccttc tttatcaatg cgcttataag
    43501 tatgtgcacc gaaatagtca cgctgtgcct ggatcaggtt cgcaggcaga acagcggcac
    43561 ggtagctatc gtaataggca accgcagcgg cgaaggtcgg aaccgggata ccgttctgta
    43621 ctgcataagc aacgacatca cgcagcgcct gctggtagtc atcggcaatt tgcttgaagt
    43681 acggagccag cagcaggtta gcgatctgcg gattttcggc atatgcatcg gtgattttct
    43741 gcaggaactg cgcacggatg atgcagccag cacggaaaat cttcgcgatt tcgccgtagt
    43801 tcagatccca gttgtattct tcagacgcag cacgcagctg agagaagccc tgagcgtaag
    43861 aaacgatttt gcccagatac agcgcacggc gaactttttc gatgaactca gccttgtcgc
    43921 ctgctgactg agcttgcgga ccagagagaa ctttagatgc ggcaacgcgc tgatctttca
    43981 gagaagagat ataacgtgca aacacagact cggtaatcag cgacagcggt tctccgagat
    44041 ccagcgcgct ctggctggtc catttaccgg tacctttgtt agccgcttca tccaggatca
    44101 catcaaccag gtagttaccg tcttcatctt ttttagtgaa gatatctttg gtgatgtcga
    44161 tcaggtagct gctcagttca ccgttattcc actcggtaaa ggtctgtgcc agttcttcgt
    44221 tagagagatt caggccgcct ttcaggagag aataggcttc agcaatcagt tgcatatcac
    44281 cgtattcaat accgttgtga accatcttca cataatggcc tgcgccatca gcaccgatat
    44341 aggtaacgca cggttcgcca tcttcagcaa cggcggcgat tttggtcagg atcggcgcaa
    44401 ccagttcata ggcttctttc tggccaccag gcatgatgga cggccctttc agcgccccct
    44461 cttctccgcc ggaaacaccg gtaccgatga agttaaagcc ttcggcagaa agctcacggt
    44521 tacgacgaat ggtgtcctgg aagaaggtgt taccaccatc gatgatgatg tcgcctttat
    44581 cgagatatgg tttgagcgaa tcaatagcag catccgtgcc tgcacctgct ttcaccatta
    44641 acaggatgcg acgaggcgtt tccagagatt caacaaactc tttcaccgta tagtaaggaa
    44701 ccagtttctt gcctggattt tcggcaatca cttcttccgt cttttcacgg gaacggttga
    44761 aaatagagac ggtataacca cggctttcga tgttgagcgc aaggttgcgc cccatcactg
    44821 ccataccgac tacgccgatc tgttgctttg acattgttta ctcctgtcag ggggtggtca
    44881 ccgccgagtg tgcggcttaa atgtggtata gatgttaact cactcataga gatggtgaat
    44941 agcattacct ttgctatacc acactgaata tctcaccttt tccgtctttt aaaacttatt
    45001 tatatgaaat tactgaatta cttgttcagt aactcaagaa taagttttgt cttttcttcc
    45061 atcagcggca catcaccgcg agattccaca ttcaaccgca ccaccggttc ggtgttggag
    45121 gtgcgcaggt taaagcgcca gtcggcaaag gtcatgctga tgccatcggt gcgatccacc
    45181 gccagcgcct cgcggctaaa atgctgttcc acgcggttaa tcgcctcaac gggttgcgcc
    45241 agtttgctgt tgatctcacc gcttgccgga aacgctgcca tccggtcgcg caccagttcg
    45301 cccagcgttt ttcctttcag gcacaccagt tcggcgacca gcagccacgg gatcatgccg
    45361 ctgtcgcagt aagcgaaatc acggaagtaa tggtgggcgc tcatttcacc accgtagatg
    45421 gcgtcttcct tgcgcatccg ttctttaata aaggcgtgtc cggttttcga catcaccggc
    45481 gtgccgcctg cggcagtcac cacatcaacg gtgttccagg agagacgcgg atcgtggatg
    45541 atcttcgcgc cgggattttt ttcgaggaat gcttctgcca gcaggccgac aatgtagtag
    45601 ccctcaataa actgcccttt ttcgtcaaac aggaagcagc ggtcaaaatc gccatcaaaa
    45661 gcaatgccca tatccgcacc gtgtttgatg accgcattgc gggtgtcgtc gcggcattcc
    45721 ggcagtagcg ggttaggaat accgttgggg aaattgccgt ccggcgtgtt gtgcactttg
    45781 attaattcca cgggtgcgcc aagggcttta aagcgggctt cgatggcgtc caccaccgga
    45841 cccgctgcgc cattcccgga gttaatcacc agcttgagcg gcgtgaggtt tttgacattg
    45901 atataaccga acaggtgatc aacgtaagcg tcacgcagat tgatttgctg atagcgaccg
    45961 cgtttggttt catcaacggg aggaaagtcg ttggcttctg ccagacgctg gacatcgcgc
    46021 agtccggtat caccgctgat cgggcgagcc ccttcgcgca ccagtttcat gccgttgtaa
    46081 tccatcgggt tatggctggc ggtaacttca atgccgccat ccacgccgag atggaacgtg
    46141 gcgaaataga tctcttcggt gccggacatg ccaatatcca gcacatcgac gcccgcatcc
    46201 tgtaaacctc tcgccagcgc cagttttaag gtttcgctgg tgaggcggac atcaccgcct
    46261 aacacaatgg ttttcggttt gagaaattcg ccataggcgc gaccaatgcg ccaggcgata
    46321 tcttcattca gttcttcgcc taatttcccg cgaatatcat aggctttaaa gcaggttaat
    46381 tttttcatgt cgttaccctt tttcaggcaa tagttggccc tgaccgaaac ggccaatttt
    46441 gtttttgtcg ttatttacat ctcggtttcg cattcattgc ctgatgcgac gtttacaccc
    46501 gtccgtagcg atccgcgaaa cgcaccacat catcctcttc gagataagag ccggagcgca
    46561 cttcaattaa atcgagcgga attttccccg ggttttccag gcaatgcatc gcccccagcg
    46621 gaatataaat ggactcgttt tcaccaagca gtttgatatc accatcaatg gtgacttttg
    46681 ccgttcccgc gacaaccacc cagtgttccg cgcggtgatg gtgcatctgt accgacaagc
    46741 cctcgcccgg tttcacggtg atgcgtttca cctggtagcg gtcgcccgcg tcgatagagt
    46801 catatttgcc ccacgggcga tagacttcgc gatgcacccg atgctcatgg cgaccatcgg
    46861 ctttgatctg ctcgaccact tttttcacat cctgcaccgc gttacggtcg gcaatcagca
    46921 ccgcatcttt ggtctgcacc actaccagat ctttcacccc gacggtggtt acgaggccag
    46981 attcggcgta cacatagctg ttttcagttt tgtgattaat cacatcgccg tggcaaacgt
    47041 tgccctcggc ggtgtgggcg ctaatttccc ataatgagga ccaggagccg acatcgctcc
    47101 agcccgcatc catcggcacc acaacggcat ctgccgtacg ttccatgacc gcgtaatcca
    47161 ccgactcttc cgggcaggcg agaaacgctt cttcatccac acgaataaaa tcgagatccg
    47221 gatcgacggc gctcatcgct ttttcacagg catcgagaat atccggacga tattttttca
    47281 gttcttcgag atagcgtccg gcgcggaaca ggaacatgcc gctgttccag taatattcgc
    47341 cgcttgccac ataggcctgg gcggtttcca gattcggttt ttcgacaaac tgcgccactt
    47401 caaaggccac cgtatcctgc tcacccgcag acacttcacc gcgacgaata tagccataac
    47461 cggtttctgg tagatccggc acaatgccga aggtcaccag cttgcccgct tcggcatatg
    47521 gcatggcatt acgcacggcg gcacggaacg cgtcttcatc ggcaatcaca tgatccgccg
    47581 ccaataccag cattaacggg tcgctctccg ggctatgacg ttttgccgcc agcgccgcca
    47641 gcgcaatggc aggtgccgtg tttcgccctg ccggttcgag aataatgttc tcggtaagtt
    47701 tgttcagttg acgcagctgt tccgcgacaa taaagcggtg ctgctcattg caaatcacca
    47761 ccgggctttc gcactccacg ccgttcaggc ggcagatggt ggtttgcagc atggtgagat
    47821 cgcctttcag gcataaaaac tgcttggggt aaagtacgcg ggaaagcggc cataagcggc
    47881 taccggagcc acctgccatc acaactggat agagtttcga ctgcgccata attatccccg
    47941 aatatcattt ataaattgac gtaacacgtt ctctttatcg agtgtgcgtt cggcatattc
    48001 acgtgccacc gtgttgtgtt tgggcagcag gagcgcctga cggatccccg ccaccagcgc
    48061 ctcgaccgat tccggttcta cgcaaaccgc aatgcccgga aaggtttcgc aaagctgccc
    48121 cagttctgtg tgggcctctg cggtaatcac cgcgttaccg cctaccgcca gaatattggt
    48181 cagtttcgac ggcaatacgg catctgccgc gccgcgtttt tgcaccacca gatggcaatc
    48241 gcccatcttc agcagtgcgg gtaaagcgtc atacgattgc agcgggaaaa attgcatgtt
    48301 gcgcagtcca cgctgctgcg ccattttttc cagccgcgct ttgccgccgc cctgcccgac
    48361 aatggcaaaa atcagcggtt catcgcgcag acgatcggca gcttcaataa cgttttccag
    48421 cccctgcttt tcaccaatat tgccggagta aagaatgatt tttttgttat ccggcaggtc
    48481 aagctggtta cgaagggcgt caacatcggc atctgcaaca tgctgaaaac gggcaatttc
    48541 cgaccagttg gggaagaaga tgacgttttc cgccgccacg cctttttcga tggctttatt
    48601 catcatcgaa cgcgaaatcg tggagacgtt atcgacgtta tgcagtccgc tacgttcgaa
    48661 cgccgttgcc agctgtgcca ctttgctgcc tttgcctttt ccggcaaggc ccagccccag
    48721 catggcgtcc acttcgtaat cctgaatatg cagcacggta cgcgcaccag aaagtttcgc
    48781 cagcaggcgc attcccggcg tgcaaaacag cgttggcacc acgccgataa tgcgatccgg
    48841 cttccagcga cgttgcgcca tcagaggaaa gaaactgctg acggcaaaac tgccgagatg
    48901 taacaggcgt ttcagggtgc tcggctgttt tggcacgtac agcgggcagc gccacaccgt
    48961 ggcggccccc tcttctcgtt tgtaccgcca ggcggaatag ttctcgccca cctgccactg
    49021 cgggtagtaa ggcggtgcgg taataacccg cacctcatga ccttgtgccg ccagccattc
    49081 caccatctcg ccggtgtatt tgccgatacc ggttaactcc ggcgagtagt taatgccgta
    49141 gaccagtatt ttcataatcc gggtactccg gcacgcttct cagcgagaaa ataggcgcgg
    49201 ctgttagcgt gaacatcgtt actggcgagc agcgcgtccg gcgtcagcca gcggtaatcg
    49261 tcatgctgct catccggcag tatcagctct tcttcagcga ctctgaagcg aaaaccgagc
    49321 accacatagt gagtggtgaa atccgtgccg gagaagttat cgtcataaaa gtgctgccag
    49381 acaccgtaaa actggcctgc tgttatcggc agacgcagcc ccagttccgc catcgtcagc
    49441 cgctcgaatg cggcttccag cgtttcgtct ttctgcacgc gccctcccgg cacaaaccag
    49501 taaccctgcg ccgggcggtt ggttcttttg ccaagcagaa actcaccgcg actgttctcg
    49561 acaataaagt cgagagagac aagcggggtg gagcgcacta ccgtggcaaa gtcttcctga
    49621 cgtaaaaaca tcattacccc cgaaagcggt cttgattctc aaggaaccac tggtaagtgc
    49681 tggcaagccc cgcttccagt gagatttcgt gataccagcc aagctgatgc aggcgcgtca
    49741 catccagcag tttgcgcggc gtaccatccg gtttgctggc atcaaaaacc acccgacctt
    49801 tgtaacccac cactttggcg atggtttgcg ccagctcgcg gatagtgcag tcaacgcccg
    49861 tgccgacgtt aatgtgcgac agcatcggct gggtgttctc cagccagact tcgtgcgcca
    49921 gctccatgac atgaatgctc gccgccgcca tatcatcgac gtgcaggaat tcacgcatcg
    49981 gtgtaccgct gccccatacc accacgtccg gtgcattctg tgccgtcgcc tcgtggaagc
    50041 gacgcagcaa tgcagggatc acatgcgaat tactcgggtg gaagttgtca tgcgggccat
    50101 acaggttggt cggcatgact gagcggtaat cgcgtccgta ctggcggttg tatgattcgc
    50161 acagtttgat cccggcgatt ttggcaatgg cgtaaggctc gttggtcggc tccagcgtgc
    50221 cctgcaataa ctcgctttct gccatcggct gttttgccag tttcgggtag atacaggacg
    50281 atccaagaaa cagcagtttg ttcacgtcgt tctgatgcgc ggcgtgaatg atgttgctct
    50341 caatcatcat gttctggtag atgaaatccg ccggataggt gttgttggca acaatgccgc
    50401 ccactttcgc cgccgccaga tagacctggt caatgcgttc gctggcaaag aaatcatgca
    50461 ccgcgcggct gtccagcagg ttcagctcgt cgcgggtgcg taataccagt tccacatcac
    50521 cgcgctgttc gagctgccgc ctgatggcag aaccgaccat cccacgatgg ccagcgataa
    50581 aaatgcgttg tttactcatg cttatgactc cagcgcgatc gccacgtcgt agccgtgaga
    50641 tttcagcaga gagtgttttt tcgccgcttc gaggtcatta gccaccattt cagacaccat
    50701 ctctctgagg gtgatttccg gtttccagcc cagtttttcg tgcgctttgg tcgggtcgcc
    50761 gagcagcgtt tcaacttcag ccggacggaa gtaacgcgga tcaacggcga taatcacatc
    50821 acccggttta acgcccggcg cgtcatgccc ggtgacggaa accacaatgc ccttctcttc
    50881 aacacccgtg ccttcaaagc gcagtttgat gcccagctgt gccgctgcca tttccacgaa
    50941 ctgacgcacg gagtactgaa cgccggtcgc gataacgaaa tcttccggct gttcctgttg
    51001 cagcatcatc cactgcattt ttacgtagtc tttggcgtgg ccccagtcac gcagggaatc
    51061 catattgccg aggtacaggc acgactccag cccctgggcg atgttggcga ttgcgcgggt
    51121 gattttgcgg gtaacgaagg tttcgccgcg gcgcggggat tcatggttga agagaattcc
    51181 gttacaagcg tacatgccgt aggattcgcg gtagttaacg gtgatccagt aggcgtacag
    51241 tttggcgacc gcatacggag atcgcgggta gaacggcgtg gtttctttct gcggaatttc
    51301 ctgcaccaga ccatacagtt cagaggtgga agcctgatag aaacgagttt tcttttccag
    51361 accgaggaag cggatcgcct ccagcaggcg cagcgtaccc atcgcgtcga cgtcagcggt
    51421 atattctggt gactcaaaag agaccgcaac gtggctcatt gcgcccaggt tgtacacttc
    51481 atccggctgt acttcacgca aaatgcgcgt caggttagag gtatcactca ggtcgccata
    51541 atgcagatgg aatttcgggt tgcaggtgtg cggatcctga taaatgtgat ccacgcgctc
    51601 ggtgttgaat gacgatgcgc gacgcttaat accatgcacc tcgtaacctt tttccagcag
    51661 aaactctgcc aggtaagaac cgtcttgtcc ggttacaccg gtgatgagag cgacttttga
    51721 catgtcttat tcctctgtat ttttgaattt attcagtttc aacgcgttcg cgtatcacca
    51781 ctgcgggatt cccccggcaa accacatttg ccggaagcga tttaaaaaca ctgcttcgtg
    51841 cacccacgac ggtgccgtcg ccgattgtga cgcctggggc aacaaagaca tcggttgcca
    51901 gccagcattt ctcgccaatc acaataggcg tggcgttaat ggtgaaatgt tgacttgcat
    51961 ggtcgtggct accggtgcat aaataacttt tttgcgatat caccgaatgt gcgccaatgg
    52021 ttatttcacc gagggtatat aaattgacgt catcgccgac ccacgcgtaa tcacctaagg
    52081 ttaatttcca cggataggta atttttactg acggacgaat aactacgttt tttcctattt
    52141 ttgctccgaa taaacgtaat aaaaaagccc gccagcgata caatacttgt ggcgaccagg
    52201 caaatattgt tgcctgtact gcccaccata attgcacttt aatagcgttg ccgccccgga
    52261 accctttcgg caccgagaaa ccgcttaaat cttgcattgg ttttccttat attcagactt
    52321 tgttatataa ggctttcgtc tttgaggtag tacgttggcg taaatgccag gataattcag
    52381 cccaaaagcc aggcacatgt aatatttgtc gttggacttt tttcgcgtca gcacacaatt
    52441 ccatattatt ggtggtagat accccaccca tggaaaattc agacaccagg ccattgagtt
    52501 ttttaaatgc ataaccggct ttatacattt tggctgccag cgcgtagtcg gaagaaactt
    52561 tatattccag gtcataacgc cattttttca agccggatac agggaaaaat atcgcctgat
    52621 gactggcggg caggctgtga taaatatacc agcccggttt ggcgctacgt ttaattttat
    52681 gcccgtcgcc aaaatccagc agcgcatcgc cggtgatcat catgttatct ttttgcattt
    52741 ttaacttacg gacaaaattt gcggcatcct gatgaaaaat atcgcccgaa ttgagaaaca
    52801 acgcgaactt gccttgtgcc atcgtaatgc ctttgttcat ggcgtcgtag ataccgttat
    52861 ctggctcgct gacaaagcgt aggttataga taccattgag attttccaga tactcacggg
    52921 tgccgtcatt ggaaccgcca tcgacaacaa tccattcgaa gctgatatct tccgcctgcg
    52981 ccagatgcgc cagcgaggca tgtgttttga ctatcccttc gaggttacga aacgcgacag
    53041 tgattatgct aagcaacatg ttcttattct tacctcgtaa tatttaacgc ttttcgcaaa
    53101 ataaatggac agacgattaa aaatgcatat tccgggctaa atatcgaacc ggtaaaaaac
    53161 agcgataccg gagtaaaaag atataattgc acgcgaaaat tgcggttatc accaaaagcg
    53221 ttgatcatca tttttatcac tttccccatg taccacagcg ataaaaacac tgcgaaccag
    53281 gaaaaataaa taatcagcag atacaaacca ttgtctatgg tttttccgac atccgcaccg
    53341 ttaaatattc cgaatgatgc gacgtattca taaagtgagc caaatctgac tacaccgtca
    53401 atatgggtca aagaataacc gaccatcacc agcggaccga caatacgata atacgatgac
    53461 gatccttccg tacctaaatc gcccagacgg gtggaaatat aaggaaacgc gattaccaca
    53521 ccaaccagga atacagccag agaaatcaat gccaacggta actttttctt aatcgcctct
    53581 ttattcagat attgaaatgc ccactccagc aaataaaaca ggataaaagt cataacccct
    53641 gaaaacgatc cggataatat tatccctgcg agaatcatag catcggtttt aggcgttttg
    53701 ataccaaact gtttgatgct gagccaaatt gagattaatg ccagagcgaa aaatgccggt
    53761 tcgaaataaa gtgctgtcgt gcgcttgccg ccgaatttaa tgaaattcag tacatagctg
    53821 ttgctgtaaa tcagatattt cgaaatcgac tccataatac tgctgccacc ggtgaggata
    53881 atttgcgcca tctccaccgc cgccagcgcc accaccagcc cgatcaccag ataaaagaaa
    53941 cgtaatatct tgcgatggtt gtgtggcgaa atcgttttaa agcgaatact ccacaccatg
    54001 ccaataatga tcacaatata gacaaacagc atggttgaag tgacgtattt actggcatcc
    54061 agcgactgac caaacagata gttaaacgcc gtgagtcccg caccaatccc taaggcaatc
    54121 atcaattttt taacgctgat gcgttctaaa aacagcagta acaagacagg taaaaaagtg
    54181 acgatggtga tggggaaact ttcgccaagc tgggcgattt tgacgttaac cagcaggtag
    54241 atcagcggca gcagcaggta gctacagatt ctgatagaag ttgacatact cctccagcat
    54301 ctgttgtcca ctgtaggtgg cgcggctgcg ttggctgaac tcagccagcg tggtaccaaa
    54361 tatcgcctgc gcgatttccg gtttgcttaa ctgcaccagt tgcagcacgt cttcttcgtc
    54421 gacggtttta ccgccggatt tttgcaacac ttcccgtgcc gcgtcgctat gggtggcaat
    54481 caccggcacg ccaattgata acgcctcaca caaaatcagc gggtagttat cgacgcgaga
    54541 actgaatacc agcgcatcca tctgattgag cgcgctcatc aacttgcgct tgtcagtttc
    54601 aaagccgtga ttaaccacgt tgccagcggt gaacggcgag aacttaccaa aggtatgcag
    54661 ttcgattttg tcgcccagcg ccatcatctc acgcaccagt tgctggttag tcttgccgtc
    54721 gtaacgcaag tcatgcgcca ccaccgcgat tttcggcttg ccctgggttt cgcgcaccgg
    54781 aggcaagtcc gccagaatcg cttcggttgc catatcaata ccattattga taatccggca
    54841 acgccctgga ccgtacaggc tattgaaagc gtcagccaca tgctggctgg gggaaataaa
    54901 ctgacagccc agcgccagca tctcacggaa taactggcgt ttgcccgcca ccagctggtg
    54961 tgcgcgatca atcttcaccg gcggatagtt atttaaggtc gggcatttct ggcagcccgt
    55021 tttccagcct tcgcaaccgt cggtaaaggc gcagcgtccg gtaacactcc agtggtcgtg
    55081 cagcgtccag accagagtga cgtccggttt atggtttttc actttttcgc aaaagcgcac
    55141 cacgctctta agatttagcc agtagctgtg cagcacatga aaatgcagga ccaccggacc
    55201 cggtgtgcga gtaatggtgc gatataactc attgaaattg ccaaacagat cgcgattaaa
    55261 cagacgaaac agggcaatat tcgccatcgc ggtcatccgc ggcgtatgtt tgatgacctg
    55321 cggatagttc tgatggctga cgctctcttt gccgcctttg ccgtaaccgt agacaaaatg
    55381 tgacgccagc ccctgttgca gcgcacgctg gtggagatct aacgccacac ctgctgcccc
    55441 gccttccgcc agtcgcacat taaattgcag aatattcatt taattacctt cactcgcgct
    55501 ttttctccca ccaccagcgc gttgtccggg acagagtcga gcaccacgct gcccgcaccc
    55561 acggtgacgt tgttaccaag cgtgatatca ccaagaataa tgacgttggc accgagttcg
    55621 acgccgttgc caatgtgtgg acatgccatg ttatcggcac cacgattgcc gatagtgacg
    55681 ccgtggcgaa tggtgaaatc atcccccgct accacgtttt tattgatcac tacggcgtaa
    55741 ccgtgatgga tagtaaagcg gcggccaatg gtcgcggcgg cctggatttc ataaccgaaa
    55801 aagcattcgg tgataatgcg atacagcacc agcagcgggg ccgcccacag attgttgagg
    55861 acgttctttt tgcgccacac cgaacaaaaa tgagcaacac gataggcaag aaccatgcag
    55921 cacgggcgta aactccagct gttggcacgc agatcttcca gcatctttag cgcccccgaa
    55981 taccatcagc cagacgtttg ccgttacgca ccgacagcag cgtcagcaaa gtgcgccagg
    56041 tcatacgttt attgcggatc tggtagaggg taaacagctg atattttttg cttgcacggt
    56101 caaatttatc tttgtgcttt cggtaaaagt ggaagtaccc ggagaatttt ttcggtgacg
    56161 aggtgatctg catttcaccg tgattgatat gcaggatctg cgtcgcttct tccactttcc
    56221 acggttcgcc gtactccacc accatccgca ggaagatgtc gtaatcctgt gctgctttca
    56281 gttcggtatc gaacaaacac tctttgaaac gccatgccca agtaaagacc tgattgccaa
    56341 tgatattgcg tttatagaac aggcggcgtg agtacggcga tttgggatac agtggtaagc
    56401 ttgccggttg ggaataaact tctccctggc atacgtagtc gttagcgtac aaaaaggcgt
    56461 gagtgaccag ttgctgttta tgggcgagga agacgctcag acggttgggt gtccattcgt
    56521 catcgtcatc gatcccggta atgtattccc cttgcgccag cataatcgcc tggttacgta
    56581 ccgcgcacgc cccgctgtta atgtcgttgt gaatgtaggt gatacgcgga tcgttgaggg
    56641 cagtgacgta ctgttgcagt tgttcccagg aggtggagca atcatccacg atgatcatct
    56701 cccagttgct gtagtcctgg cgcaggaccg attttatcgc ccgaatcgcc agttgttggc
    56761 ggttccaggt cggcatatag attgaaatca gcggattctc tttcataggt tgttctcccg
    56821 gcttatgagc tggtttgctt gccggatgcg gcgtgagcgc cttatccggc ctacggggcg
    56881 gtgcgaatgc aggcgtcgtt atttcgcatc cgacttatat tcgtattcgt aatagccata
    56941 atcctgatac gcgctggcgc ggcggaagat ggagttcaga atcacccctt tcaccggaat
    57001 accgttttgc tcaaagcggc tcagactggt ttccacttct ttcaatgtgt tgaccgcata
    57061 gcgcgccacc attaacgtgg ttccagcgtg acgcccgaca attgctgcat cggtcactgc
    57121 cagaatcggc ggcgtatcaa tcaacaccag gtcgtagttt ttactcgccc agctcaccaa
    57181 ttcggcaaag cgttcgctca tcaacagttc agaaggattt ggcggcacct gaccgcgcgg
    57241 gatcaggtca aatttggcaa tagaggtcgg tttagctgcg gtagtaatat cgccctggcc
    57301 aatcagaatt tccgacaggc cattgacgtt attggtaccc aacagctcgt gggtgtagcc
    57361 tttgcgcata tcgcagtcga tcaacaacac ccgtttattg gtctgactga tcaccgccgc
    57421 caggttggcg cagacaaagg ttttaccgat tgacgggctc acccctgtca tcatcaacac
    57481 attgttctgc gcctgcatca tcgcgaagtg cagactggta cgcagactgc ggatggcttc
    57541 aatcgccaga tcggttggat tccccaccgc cagtagctgg ctctgtttat agcgtttaat
    57601 ccctttgatg gttttgacgc tatcgcgcgc tttctgccat tccgacagcg ggatgctggc
    57661 atagacgctg ataccatgtt cttccagcac ctgcgggctt tcgatgccgc gattaaacaa
    57721 cgagcgcagc agcacaccca cgatagagag catcaggccg aggataattg cgccgaggat
    57781 aatcagtcct ttcttcggtt tcagcacgcc aggctgggtg attgccgggt caacaatgcg
    57841 cacatcgccg acggtgctgg cttcggtgat tttcagctcc tgctctttat tcagcagttg
    57901 catatagacc tgctgaccag actcgacatc gcgggtcagg cgcacaatct cctgctgggt
    57961 tttcggcatc gccgttacgc gaccgttaag tttggctttc tcttcttcca gcgcctgacg
    58021 tttctccagc agcgtgcggt acgccgggtg aactttggtg tacagcttgg agatttccgc
    58081 ctctttaaag gtcagttcgt tcaactgcgc gtcgatgttc accatcgaat cgagcaccgc
    58141 tttcgcttcc agcggcagat caacagaatc tttatcctga cggaaggcat tcagtttgtt
    58201 ttcggcaaca tcgaggcggc tacgtacttc cggtaactgt tgcgcgagga aagcgaggct
    58261 tttcgacgct tccgccgatt tgcgctcaat attttgttcc tgatagttac gggcgatgct
    58321 gttaagaatg tcgcggatct gttcgcgatc ttcaccggta taagtcaggc tcagtacgcc
    58381 tgcgtctttg ccgttctccg ttaccgtcag gctgttttgc agttggttga tcatccccaa
    58441 cgtggagtat ttggtgacgg taaactcact gcccgggcgg gcgtgaatgg cttcaaccat
    58501 cagcgtgacg ccttcttttt tcagcatctg gcccgcttgc ccacgggcgc taaagccgcc
    58561 atcactgctc agggtgtagt ttttattgtc cagcacatta agcgtaaaca cctggcccgc
    58621 catctctttc gggcggttaa aggtagtcac tttcaccgtc tcgttctggc gtcccatcag
    58681 tcgctcccag cccgccccga aaatcgggaa cgtgtttttg ctcactgcaa tatcgaggtc
    58741 gagatcgtcc accgttttac caagcaccag acgcgaacga atcaactgga tctcggcgtc
    58801 cgatgcaggc ggtttgttgg ctaatgccga accaatgtcc tgcactaacg aattgccgct
    58861 gttttgctcg atttgtacca gtgcgtcggc actataaatc ggcgttgcga agaaggtgta
    58921 aaccacggca cagagggcaa atacagcggt gatgccaatc acccaccagc gcgcttcaat
    58981 gacggtgccg accaggcgac caatatcgat ttcatcactg cccgttaccg gagcggcatg
    59041 ttgttttact ttttctgtca ttcttatacc tgctctgcgt tcaatgcctg cgcccactgg
    59101 cgggcagacc gttcaagtaa tgtgtacacc gctgcaaacg tttcccggct tttgcgatac
    59161 ggatcgggga tttcacattc gttatcccag tgaccaaaca gcatcacttt gccgcgcatc
    59221 tcaggtgcca tctcgcataa gcgttcgata tggcgctttt ccatggtcaa aatcaggtca
    59281 tagttgcgac acaggcggcg gctgatttgt cgtgcacagt ggccttccag agacagttga
    59341 tgttctgcgg cgacgctgat agcggtagga tcagcgccct taccgaccag cgcgccgagt
    59401 ccagcggact ccactttcag ttccgggtga taacgttgca gtaagcgttc cgccgtcggg
    59461 gaacggcaaa tattgccgac acagacaact aatatgttgt taaacatgac gattaccagt
    59521 tatgaatgtc gctggctgta tccgtcatgt aacggacacc gctaatagtt ggcaacaact
    59581 gattgatcag acggttccag cgggaaaccg gggcggtggt gacatacacc acgtcataag
    59641 gttgcaggcg gaattctgtc gccatcacca gcgacgtggc atcggacata tccagctggt
    59701 agatattggc aatcttgcca ttacgcccgc cctcgccttt cagcggacga atgacaaaga
    59761 tgccgctggc gttggaggtg gtcatgtcga taccttcagc attacccagg gcttcagtca
    59821 gggtcatgcc gctaaagtcc attttcaggg tgctctgttt cttcacttca cccatcacaa
    59881 atactttcag atcatcatta cgcggcacgt agagaatatc gccgggataa agcagtcggt
    59941 tctgattgag gtcgccgttt tgcatcagcg cctgcaaaga aatgcgttct tcacgaccat
    60001 tgtgtgttag cactacgttg cgccagtcag cggtgtcggt caggccacct gcggcgttga
    60061 tggcgtcgag aatagtcagt ggcacgttgg tgatcgcctg ttgaccggat ttattcacct
    60121 gaccggagat ataggccttt tgtgagcgga aggcggcgat attaacgtcc acctgcgggt
    60181 cagcgatgta cgtcgctaag cgcccggtaa tatcactgcg gatttcagcg agcgttttcc
    60241 cgactacgtg aaccttgccg atatacgggt aaaacatagt gccgtcaggc tgtacccagt
    60301 tgccggtgtc gctggagctg cggtactgac cggctggcgt ggtgagttcc gggtgatccc
    60361 agacggtgac attcagaacg tcgcccggcc cgacgcgata ctggtaattc gcgatctcgc
    60421 tttccagcgt catattgggg cgcgctacat tcgggcgtgg gcgtaattgg tcaatcaggc
    60481 gcggggtcag cggataaaca ttcaccattt tgtcgagatc gaaatcagcg tcctgctgtt
    60541 tgatgacgtc tttgcccatc gtcgacatat tgctgcccgg aagtactgtg caaccgctta
    60601 tcaaggttac tgacaccaat aatggcatca atttcatttt ggatttcatc attgtttatt
    60661 tatcactttg gcagagtaat tatcctgtgc actattaata gcaatatcgc catgcacatt
    60721 taccttgcag ttaattgaat aaaaatttaa ctggcatcag tcctaaaaaa attgatttca
    60781 tccgcaggct attgacagaa taattcagac tggtctttca ggcatccaga cacgctaccg
    60841 cccctggctt tttagccacc aatacactga tttagcttaa tttttcaaac cctctctgca
    60901 tacaatcgtt gatgagaaag ggttattgcg gaaattaact tccgaatata aggtgacatt
    60961 atggtaattg aatattggct ctccaataat gcaggaggaa gtgttacagc taacggaata
    61021 gcaggcaagg taacaattcg gcaattggct atttttaaga attatattaa gtgtcattca
    61081 atatggtttt taggagtttc tttaggttga caatatttaa tattgcgcct ccacatgcga
    61141 tatttcttaa ataatgtttt attattacca cttttaattc agggataatg tgaggttatt
    61201 accctcaaat aatatgagat aaatagtgct gcaacattgc atttttgccc cgatttatcc
    61261 atgatcgaat tgtgacattt gtcatacaac gaataggttt tgtacttact atggaatgga
    61321 ttgccgatcc gtctatctgg gccgggttaa tcacgctgat tgtgatcgaa ctggtcctcg
    61381 gcattgataa cctggtcttt attgccatcc tcgccgaaaa actaccgccg aggcagcgcg
    61441 accgcgcacg ggttaccggg ctgctgctgg cgatgttaat gcgcctgtta ctgctggcgt
    61501 caatctcctg gctggtcacc ctgactcaac cgctgttcag cttccgctcg tttaccttta
    61561 gcgcccgcga cttaatcatg ctgtttggtg gtttcttcct gttgttcaaa gccacgatgg
    61621 agctgaacga acggctggaa gggaaagaca gcaataatcc cacacaacgc aaaggtgcga
    61681 agttctgggg cgtggtgacg caaattgtgg tactggacgc cattttctca ctcgactcag
    61741 tgattaccgc cgtcgggatg gtcgaccatt tactggtcat gatggccgcc gtggttatcg
    61801 ctatcagcct gatgttgatg gccagcaagc cgttaacgca atttgttaac agtcacccga
    61861 cgatcgttat tctctgctta agcttcctgt tgatgattgg ctttagcctg gtggcagaag
    61921 gtttcggctt cgtcattccg aaaggctacc tgtacgctgc cattggtttc tcggtgatga
    61981 tcgaggcgct caatcagttg gctatcttta accggcgacg ttttctttcc gctaaccaga
    62041 cgctgcgcca gcggactacc gaagcggtaa tgcgcctgct tagcgggcaa aaagaagatg
    62101 cggaactgga cgccgaaacc gcgtccatgc tggtggatca tggtaaccag cagatcttta
    62161 atccgcagga acggcggatg attgagcggg tacttaatct taaccagcgt accgtcagca
    62221 gcattatgac gtcgcgccac gatattgagc atatcgatct caacgcgccg gaagaagaaa
    62281 tccgccagtt gctggagcga aatcagcata cgcggctggt ggttaccgac ggtgatgacg
    62341 cagaagattt gctcggtgtt gttcacgtta tcgacctgct acaacagtct ctgcgcggcg
    62401 aaccgctcaa cctgcgggtg ttgattcgcc agccgctggt gttcccggaa accttgccgt
    62461 tgttacctgc cctggagcag ttccgtaatg cccgcacgca ctttgctttt gtggtggatg
    62521 agtttggctc ggtggaaggg attgtgacat taagtgacgt cactgaaacc attgtcggta
    62581 acttaccgaa cgaagtggaa gagatcgacg cccgccatga tattcagaag aatgcggacg
    62641 gttcctggac ggcgaatggt catatgccgc tggaagatct ggtgcaatat gtgccgctgc
    62701 cgctggatga aaaacgtgaa tatcacacca ttgccgggct gttgatggaa tatttacagc
    62761 gtattccaaa acctggcgaa gaagttcagg tgggggatta tttgcttaaa acgttgcagg
    62821 tggaaagcca tcgcgtgcag aaggtgcaga ttattccgct gcataaggat ggcgagttgg
    62881 agtacgaggt gtgatggtat cggtcatttt acccaatagc cgaaaggaat aagatgaggg
    62941 aaccgcgcca gcggcgattt accgccggga gcccgggttg ccagggtggt ggcgattgag
    63001 ccaccctggc acgttcaccg gcttgagcgt tccatagtag cgaggaacat caagtgaacg
    63061 gaacagcctt cgatcttaca tattcagaat ataatgatgc cgccacgaac gcctgatgcg
    63121 acgctttacg cgtcttatca ggcctacagg actggtcacc tggtaaccta catcttctcc
    63181 agcaacttct tcacatcctt gccattgcgg gaatctttat tccgctcggc ccagtcattc
    63241 aggcgacgtt tcgcttcgtc ctgtagatgt ttgcgcagta actgatccac ttgcaggctg
    63301 taattgagtt gctgccagtt gccataaacc cgcagcggta ctggcgtttc tttcaggaaa
    63361 tcaatcagtt tactttcccc gttccagcca cccacgaccc gaatatcaaa ctgggtatcg
    63421 caggtttgat ctgccagatt caacatgcct tcccctgtca gcgccagcac aggcgattga
    63481 ccttgcatgt cgtttaacgt cacgacgcca tccttcaacg tcaaatcggt ggtaaagcgg
    63541 tcaagccgcg tcacgttatc aaagttttca gcggccttca catcaccgcc attacgctct
    63601 accgcttgct gaatcatctg ctggaagttc atcccttcca tgcgcgtgtc ggtcatttcg
    63661 acatgtgcct gtccttgcca gttgtggcgg aatgcgtcgg catctatgtc agcaccggag
    63721 aaatcaccag ccagtgacat ttttccggtc aacgaaatcg gatagttaaa cgccttcaga
    63781 atggtaccaa tctcaacgtt ttccagccgt ggctggaagt ttatccgcgg atttattgat
    63841 gtcgcgtcca gcgtgcccgg cagtgaaacc tgtccaccgt taagtttgcc ctgcagttga
    63901 gtaatttcca gcaaacccga cttgttggtc atttgcgtgg caacatctgt aaaattcatt
    63961 ccgcgccagc gcacgttact ggcctgcaac aaaatatcag ccgtaaagcc ttgcagtccc
    64021 tgataggccg gttcatcaat acgcgaagaa atgaccgggc gcggcaacgt tgattggctc
    64081 tgcccctgct gcgcggcacc gttttcacca ttcgcggttt cattaagcgg gatgaggttg
    64141 tcaagattca gttgcgggaa ttgcagtctc agctgccatt ccggtttctc tgtcagcgtg
    64201 acctgtgctt gcccgctcag cgtactgtca ttggcggtca agctgatttg gttaaatgaa
    64261 aggcgtttat gtgactcctg ccactgggcc tggaaactcc cctgcccctg aataccttgt
    64321 tttggtaaat cggcaccctg caactgccag ttaatttgtt caatagccgc cgttaaatca
    64381 tgcggataat cagaagcatc caccgtaccg ttaagggata ttgtgagatc gcgctgatcg
    64441 cgattaaccc gcccggagaa ctcaaatgag ccacgatgtt ggggatcttg ttccatttgc
    64501 aggcggatat tgcggattgt cacctgctcg tcatcttcat gctggaacac cagcacgctg
    64561 tccgccacct taagactgga tatatcaaac gaccatccgc gatcgtctga cagatccggc
    64621 aaggtattgt cgcgcggtgc aaccggagcg tcttcactgc gcaccgcttc cgtctgcggc
    64681 gtcagttgga tcactgcccc ttttagcatc acctgcttaa cgctcagttg atgactcagt
    64741 agtggtaaaa gcgccacgtc cagacgcatg ttgtcggcgc gaaccagtgg ctggcttgcg
    64801 ccctgggcgg tgagagacat tcgcccggag aggatactaa gctgcggcca gacgtgccaa
    64861 cgcagtggcc cgtcgagctg caattgataa ccgctacgtg cagcaacttg cttgaccata
    64921 tagtcgcgga aatcattcgg attcaccagc aacactaacg cagataaccc ggccaccagc
    64981 acgaccagga gtatcatcag cgtcgtcaga aatcgtctca tggtatcctc aatgggcctg
    65041 aattagtctt tatcgattcg gctggctacc gcgccctgct ggttgcgata tttcgcatct
    65101 tcacggcggt tgtaaggtcg cgccgccggg ccggaaagcg gctcaaagct cagcgcacca
    65161 attaacatgc ccggacgcag cgccagcggc agcttaccgg agttgtagaa ctccagcaca
    65221 atgcaaccag accagcccgg atcgatacgg tgcgcggtga cgtgcaccat cagccccaga
    65281 cgcgccaaag aggaacgccc gtccagccag cccaccagat cggctggcag cgtcaccgac
    65341 tccagcgtca ccgccagcgc cagctctcct gggtgaagat agaacgcctc gccctcgtcg
    65401 agaacgatct catcgctcat cacgcggtca agcgcggcgc tcacttcatc tttgggaccg
    65461 ctcagatcga taaacgctgc cgtgtgacca cggaaggtac gaaatttatt gcccaggcgt
    65521 acatccaccg tcgcgccgtt aatacgctcc actggcggac gtgggttgat cgacaaacgg
    65581 ccttcatcaa gccaggcttc aatatctcgg tcacacagac gcatggcatt ttctcctttc
    65641 gcgcatcact cccttaacgc caattacgtc aagggcatac caggttatca ctgaacggta
    65701 cacaatttat caagcttatt caaagaactg acttattttc gctttcaata tatcgatcgc
    65761 gatgcggttt ttcccgccgc gcggcacgat aatgtccgca tattgtttag aaggctcaat
    65821 gaattgcagg aacatcgggc gcacggtttt ttgatattgc gccatcacgg aatccattga
    65881 acgcccacgc tcgttaacgt cacgcttgat gcggcgcatc aggcagatat ccagcggggt
    65941 atcaacgaaa atggagaagt taagttcgtc acgcaaacgc gcatccgtca gcaacaaaat
    66001 gccttcgaga atgatgacct tcttcggctc aaccgtcacc gtttctttca tacgcgtatg
    66061 ttcaacatag ctgtaaaccg gcaggtcaat tgccgagccg cgtttcaacg cttgtaaatg
    66121 ctcaagcagc aggctgtgat ccatcgcgct gggatggtcg tagttggtct taacgcgttc
    66181 ttccatcgac agatggcttt gatctttgta atagcagtct tcgggaatta cgccgatgtg
    66241 ttcatcaccg acttgctcac gtaattcacg ataaagggta ctggcaataa gactcttgcc
    66301 ggaagccgat gcgccagcga taccgataat gacgcactga tgagactgat cagtcatata
    66361 tttagcgacc tgattaacct ggatgttagg aagggggcga cgaagcgcca aacgcggcaa
    66421 ttatagggat ttcatccgtc tgataccagt cgaatagcgt tgccgcgcgc tcagagttaa
    66481 ttgttgacaa agaattcccg gtggcaaatt acgttgatca gttttatgta aggtaaaaaa
    66541 tgttatacgc agttgcgcaa attatccgcc tttacgtcac tttatgagca attcgcatat
    66601 aaaatgtaaa acttttgtac tagcataaac acagaaacga atactggcga cctggtcttg
    66661 cggataaagc ggtaatgagc aaacaatcac agcatgtatt aattgccctg ccccacccgc
    66721 tgcttcacct ggtcagttta ggtttagtct cgtttatctt tacccttttc tcgcttgagc
    66781 tttcgcagtt tggcacccaa ctcgccccac tgtggttccc gacgtccatc atgatggtgg
    66841 cgttttatcg ccatgccggg cgcatgtggc cgggaattgc gctgagctgt tcgctgggaa
    66901 atatcgccgc atccatcctg cttttttcca ccagctcgct gaacatgacc tggacgacca
    66961 tcaatattgt tgaagccgtg gtcggggcag tgctgctacg taaattgctg ccgtggtata
    67021 accccttgca aaatctggct gactggctgc gtctggcact cggcagcgcc attgttccgc
    67081 ctctgttggg gggtgttctg gttgtcctgc tgacgcccgg agacgatcct ctcagggcat
    67141 ttttgatatg ggtactgtca gaatccatcg gcgctctggc actggtgccg ctgggattgt
    67201 tatttaaacc acactatctg ctgcgccatc gcaacccacg gttgcttttt gagtcgctgc
    67261 tcacgttagc catcacactg acgttaagct ggctttcgat gctgtatctg ccgtggcctt
    67321 ttactttcat tattgtgctg ttgatgtgga gcgccgtgcg cctgccacga atggaagcct
    67381 ttttgatctt ccttaccacg gtgatgatgg tgtcgctgat gatggccgcg gatccctccc
    67441 tgcttgctac gccgcgtacg tacctgatga gccatatgcc gtggctaccg tttttgctga
    67501 tcctgctgcc cgccaacatc atgaccatgg tgatgtatgc ctttcgtgcg gaacgcaaac
    67561 acatttccga aagcgaaacc cgctttcgga acgcgatgga atattccgct atcggtatgg
    67621 cgttagtggg caccgaggga caatggctgc aaaccaacaa agcgctctgc cagtttctcg
    67681 ggtacagtca ggaagagctg cgcggactca cctttcagca actgacctgg ccggaggatc
    67741 tcaataaaga tctccaacag gttgaaaagc tgataagcgg tgaaataaac acctattcaa
    67801 tggaaaaacg ctactacaac cgcaatggcg atgttgtctg ggcgttgctt gccgtctcac
    67861 tggtgcgcca cacggatggc acgccgctct attttatcgc tcagattgaa gacattaacg
    67921 agctaaaacg caccgaacag gtcaatcagc aactgatgga gcgcatcacg ctggctaacg
    67981 aagcgggcgg gattggcatc tgggagtggg agttgaagcc gaatgttttt agctgggata
    68041 agcggatgtt cgagctgtat gaaattcctc cacatatcaa accgaactgg caggtgtggt
    68101 acgagtgcgt gctgccggaa gatcgccagc atgccgaaaa agtgattcgt gattcgttgc
    68161 aatcacgctc gccctttaaa ctggaatttc gcattaccgt aaaagacggt attcgccata
    68221 tccgcgccct cgccaaccgg gtactgaata aagaaggcga agtcgaacgt ctcctcggca
    68281 tcaatatgga tatgaccgaa gtgaaacagc ttaacgaggc attgtttcag gaaaaagagc
    68341 gcctgcacat tacgcttgat tccatcggtg aagccgtggt ctgtattgat atggcgatga
    68401 aaattacctt tatgaatcca gtggcggaga agatgagcgg ctggacgcag gaagaagcgt
    68461 taggtgttcc gctcctgacg gtgttgcata ttacttttgg cgacaacgga ccattaatgg
    68521 agaacattta cagtgccgac acctcacgtt ccgcaatcga acaagatgtg gtgttgcact
    68581 gtcggagcgg cggcagttac gacgtgcatt acagtattac gccgttaagt actctggacg
    68641 gcagcaatat tggttcggtt ctggtgattc aggacgtcac cgaatcacgc aaaatgctgc
    68701 gccagctgag ctacagcgcc tcccatgatg cactgacgca tctcgccaac cgcgccagtt
    68761 ttgagaaaca actgcgtatc ctgctgcaaa cggtaaacag tacgcatcag cgacatgccc
    68821 tggtgtttat cgatcttgat cgctttaaag cggtgaatga cagcgccggg catgcggcgg
    68881 gcgacgcttt gctgcgcgaa ctggcgtcgt tgatgctgag tatgctgcgc tccagtgacg
    68941 tgctggcgcg gctcggcggt gatgaatttg gtctgctgct gccagactgc aatgttgaaa
    69001 gcgctcgttt tatcgctaca cgtattatca gtgctgtgaa tgactatcac tttatatggg
    69061 aaggccgggt acatcgggta ggtgccagcg ccgggattac cttgatcgat gacaacaatc
    69121 atcaggcggc agaagtgatg tcgcaggctg atatcgcctg ttatgcctcc aaaaatggtg
    69181 gacggggccg ggtgacggtt tacgaaccac agcaagctgc cgcacatagc gaacgggcgg
    69241 cgatgtcgct tgatgaacag tggcggatga ttaaagagaa tcagttgatg atgatcgccc
    69301 acggtgtcgc ttcgccgcgg atcccggaag cgcgtaattt gtggctgatt tcgcttaagc
    69361 tctggagttg cgaaggcgaa attattgatg aacaaacatt tcgtcgtagc ttcagcgatc
    69421 cggcacttag ccatgctctt gaccgacggg tattccacga atttttccag caggccgcca
    69481 aagcggttgc cagtaaaggc ataagcatcg ccctccccct ttccgttgcc ggtttgagta
    69541 gcgccacgct ggtgaatgat ctgcttgagc agctggaaaa tagccctcta ccaccacggt
    69601 tattacatct gattattccg gctgaagcga ttttagatca cgcagaaagc gtgcaaaaac
    69661 tgcagctggc gggatgtcgg atagtgctca gccaggtggg ccgcgatctg caaatcttca
    69721 actcgctgaa agcgaatatg gcagattacc tgctacttga tggtgagtta tgcgccaacg
    69781 tgcagggtaa tttgatggat gagatgctga ttacgattat tcaggggcac gctcagcgac
    69841 tcgggatgaa aaccatcgcc gggccagtcg ttttaccctt agtgatggat acgctttctg
    69901 gcatcggcgt cgatctgatt tatggtgatg tgattgccga tgcccaaccg ctggatttgc
    69961 tggtgaatag cagttatttc gcgattaact gagagagcgt ttgcccattg cctgatgcga
    70021 cgctaacgcg tcttatcatg cctacaaatc gctcattccc cagaccggat aaggcgctcg
    70081 caccgcatcc ggcgaccaac gtcatgcttc gtctggttgc cagccttccg tataccagat
    70141 atgcaacagc gcataagaac gccagggctt ccagcgctcg gcataacggc ggatttgtgc
    70201 cggtgtcatt cccggaaatc gccgtttaat cagataatca tccggcagaa aaacatcttt
    70261 cgcctgccag ccacgcaaag caaaataatt cgccgtccag cgcccgatac ccggaaaagt
    70321 ttgcagcgtt ttcatcgcct gctccacatc gcccggaatt gtcattggta agctgccctc
    70381 cagcgccgca tttgccagat gaatcagtgc ctctgcccgt ttcaacggca tgcctaacgc
    70441 ttttaatgcc tgcgggtcgg ctgctgccag ccgctgaggc gtggggaagc agatatactc
    70501 cggaaaatca tccagccgtt cgccataaag ctgtaccact ttggcagtca attttgccgc
    70561 catcgccacg ctcaccagtt ggcctaaaat cgcccgcaca ccctgctcaa aagcatcaat
    70621 acagccgggt aaacgcaatc cgggccgccc cgcgcctaac tttcccaacg caccgttaac
    70681 aatctgcggg ttacattgca gatcaagcag gcggctcatt ttcgccagac actctgcggc
    70741 aacaggttct aaacctgcac ttaaatttat gtgcagagta tggcgggcta tatccggaat
    70801 agcagtcact acgccgcgat attcgcccac cgccagacta cgggcataat aattgtccgc
    70861 gaccgtttcc acaccgctca ccgcacgggc ggcgagaaat cccaacatcc acgaccagtc
    70921 atacggcggc tgccagttca gggtatacat cgcatctcct tgttaatccg ctttcagcat
    70981 aaacgttatt cagacgttac gctttgcttt catattccgg ttgtcgcgac agcaacattt
    71041 cgctaaagtc acgccccttc ttcaccggca tggggattaa ttcgtgttta ttggttttga
    71101 ttacggtaca gcaaactgtt cagtggcggt catgcgtgac ggtaaaccgc aattactgaa
    71161 aatggaaaac gacagcacgc tgctgccttc aatgctttgc gcgccaacgc gtgaatcggt
    71221 aagcgaatgg ctgtaccgcc atcatgatgt tccggcagac gacgatgaaa cgcaggcgct
    71281 gttacgtcgg gcgattcgtt ataaccgcga agaagatatc gatgttacgg cgaaaagcgt
    71341 gcagttcggt ctttcctcac tggcacagta cattgatgat ccagaagaag tgtggtttgt
    71401 gaaatcacca aaatcgttcc tcggtgccag cggcttaaaa ccgcagcagg tagcgctgtt
    71461 tgaggatctg gtctgcgcaa tgatgttgca cattcgccag caggcgcagg cgcagctgcc
    71521 agaagcgatt actcaggcgg tgattggtcg cccgatcaac ttccaggggc tgggcggcga
    71581 tgaagcaaac gcccaggcgc aagagattct ggaacgcgcg gcgaagcgtg ccggattcag
    71641 ggacgtggta ttccagtacg agccgatcgc ggctgggctg gattacgaag ccaccttgca
    71701 ggaagaaaaa cgggtgctgg tggtggatat cggcggtggt acgactgact gttcattgct
    71761 gctgatgggg ccgcagtggc gttcgcgtct cgatcgtgaa gccagtctgc tgggtcacag
    71821 tggttgtcgt attggcggta acgatctgga tatcgccctg gcgtttaaaa acctgatgcc
    71881 actgctgggt atgggtggcg aaaccgaaaa aggcatcgcc ctgccgatcc tgccgtggtg
    71941 gaatgcggtt gccatcaacg acgtacctgc gcagagtgat ttctacagta gtgccaacgg
    72001 tcgtctgctt aacgatctgg tacgcgatgc ccgcgaaccg gaaaaagtgg ccctgttaca
    72061 gaaagtctgg cgtcagcgtt taagctatcg cctggtgcgt agcgcagaag agtgcaaaat
    72121 tgctctttca agcgtagcgg aaacccgcgc ctcactgccg tttatcagca atgaactggc
    72181 tacgctgatt agccagcgag ggctggaaag cgccctcagc cagccactgg cgcggattct
    72241 ggaacaggtg caactggccc tggataacgc ccaggaaaaa ccggacgtta tctacctgac
    72301 cggcggtagc gcccgttctc cgcagattaa aaaagcgctg gcagaacagt tgccgggcat
    72361 tccgattgca ggcggcgatg actttggctc cgtcaccgcc gggctggcac gctgggcgga
    72421 agtggtgttt cgttaattga aaaatggctc agcagcggct taaatctgcc tgcgcctgag
    72481 ccagtttttc tgctgcctga cgtaacggtt ccatcaacgg catggtccgt gctggagcat
    72541 gaaggcggaa tcgctgcaac tctgtcgcgc cgactgtcaa cgccgattcc agccgatgtc
    72601 gtttggcggt cattttcgcc attaccgctt gtctgtctgc tggcgttatc gcttcattcg
    72661 gcctgaacac aaaacggcgt tcacagttcg ctttccagtc gataaccgcc tgggtcagat
    72721 ggtcgccaaa ccctttcact tgcttaacgc cacgacgggt aacatctgcg gctgtttcaa
    72781 taccaaaaga ccgtaacgcc gctttacgcg cagggccaac gccgggaata gaggcaacat
    72841 caataaaaaa tccctccaga aacttctgct tctgccgttc ccttgcagtg tcatgaagtg
    72901 ctgccagagc gcgtttctct tcttccggta acccgagaat ttcgtccttc attttttcga
    72961 gcatcgtccg tttggcgata aaaccttcca ggccgcctac ctgctggatc tggcggacta
    73021 aatggtcata gtccattttc gcacgattaa atgcctcacg gcgttgctga acttctgctt
    73081 tgtacgcttt gcttgtcaaa ctgccaataa tccagatagc cgccagcaca ggaaccaaaa
    73141 taatataacg cggttctgcc tgaaggccgc aaagcagcag cgataatgct gacagtgcga
    73201 tctcaagcag aatgatgtat tcgcgccgta acaggcctaa aggaagcggc ctgccagtcg
    73261 gttggaaatg atcgggtaat ggcagttgca atgctggcgg tgctactgac gccatcacca
    73321 tcgcccagac ttttgccagc acaaaatccc cgccggtggt aatgacctct tcgccgagat
    73381 caataaaata gataacacct tgattatcca gcgcacacca tgggcagtca gccaaatgac
    73441 cgggataaac atgcattgcc gaaacggtac atttctttaa ctgttggcgt agagaatcca
    73501 gtgctgatac ccacgcttta gccgtcggac gcccggttgc cacgccactt tccgtaaacg
    73561 cctgctgaaa catggcttca acatcactcg gtaacatcga tagcggaatc gatcgtggcg
    73621 gcggttttaa accacgtcgc tgattatctg acgcgtaggc ataacggaaa tgggtaatat
    73681 ccgtctccag cgcattaccc gcatcagaga taagcggcac accagaataa ggatgccggc
    73741 caccaaacaa gacgtgaaaa atcagcaacg caaggccaaa attatcgtga ttttctgtgc
    73801 gttcaaagcc gacaaatgac gacaaggttt gtagctctgg cggcgtaaaa tgcgacacgc
    73861 cgacttcgca taaatgcagt gtgccattgg cgttaatctg aaaggagtcg ctatcaatca
    73921 acaccacttt gctgtcgcga cctaccataa aactgttctg gtttacgtca cccacgacgt
    73981 gcccgtgctc gtgaaccgta gcaaaagatg aagcaatatt gcgcgcaaca tagagtagaa
    74041 aatcccacgc acaatgaggg taacgttgac gacgatgtgc cgggctatag atcatatgaa
    74101 tcggttcttt accagaaact tttggcatca taaaaccgat aacttttccg cctcgtccac
    74161 cgtgaagcgt tgcctgcggc cagacgacat aattcaacaa ttgcgcgtca gctgtcgcag
    74221 ccataaaggc aagtttgtcc tgttttaagg cgggtggcgg cgtgtgataa atcttggcga
    74281 cgctatcgac aaactcattg atatcataaa ccgcgccttc accgcctttg cccagttcac
    74341 ggcctggcgt aacgcactca ccagtagcag tatataaagt gggtttcatg gtaaccacag
    74401 cgccagtgcc aacgttttat catcatccgt acgctcgttt accgccgggc tggacaaaaa
    74461 ctgctttaac aattcaggta ataaatcgag ttgttcctgc gttgctgacg ccagtccatt
    74521 gaaaaacggg gtaaagaaag gcacatgagg agaattatcc agcatattta acgccagccg
    74581 ctgaatacca tccgtaaatg ctgcaacttt atgcgcacgc ccagtgctgg tgaaagtttc
    74641 cagtctggaa acggcatctt cgtcggtaat gaagtgcgtc atgttggcat attcacccac
    74701 catcggcgtg agaggcaatt gcagaccgtg acccaaatct accaccacgc cgccatcgcc
    74761 aatctgcata atcaaggtgc cgtcaggcga tgaaatcaat cccagaaacg tgcaggcaaa
    74821 atcgcgaact gctaatgctt tggcttctgc ctcggcaaaa agtcgttgac gaatagtcag
    74881 taccatattt gtggcgagga catcattaag ccccaattca ccgtcctgta ctttttgcac
    74941 cataaacgcc atcgcttcat tgacggcgag cgtcgcgcct tctcctccct gcgaaacgct
    75001 acctgcgcca tcagcaagga acatcaccag taatggttgc tggtcgttta accagacaac
    75061 ctgcatctgg caggcatcct gacaaggcaa atcagcacta atgtgcgatg ttccaacagc
    75121 ggatgcataa accagacgcc agctcacact gacgtccagc cttttggcgc ttccagcaca
    75181 acttccgttc ccggcgtgga gcgggaaacc gaacgcagcg aactggataa ccagctaaac
    75241 agttcacgga attgtaatcc ctgcagtggc aaaggctgac gaacgctgat ttgtgccaga
    75301 gtcttcatat ccgccccctg aacaccgatg gaaaagaagg caaacttctt atcttcttcc
    75361 ccctgaaaca ctttgttggc ggcagcctgc cactcatcgg ttggagcgcc atcggtgatc
    75421 aggaaaatcc acgggcgata atacgaaata ccatttgcgc ggtactcacg tttcctctct
    75481 tcaaccatat ccagggcttt ggtaatggcg gcccccatag gcgtatcccc ctgggcaaac
    75541 aggatgggcg ggaaaaagtt agcggcgctg gtaaagggtt gttccacatg caccgggccg
    75601 aacgtcacaa tgccgagttc cactcttttt aaagccagcg gatcagcaag cagttcatca
    75661 cgaaaggtaa ccaatccggc gttaagttca ttaataggtc tgccgctcat cgagccagaa
    75721 acatccagca gcaaaatgca ggggcaacgt ggttccgggt tactggcaaa atcgctggtg
    75781 gcaaacgtga tttgttcgct cataagaaat cctttcttag agaattatcg aggtccggta
    75841 tcataccgat tgcaataatg aaataagagc aaacgaagaa actttcagaa ttaggcccgt
    75901 cggcgcagaa aattttgcta ttcgcctcgc aagaacggag agccgtcgat tgacactaag
    75961 ggcggagtga cataatttca ggagtgaggg ttagggagag gtttccccct ccccctggtg
    76021 ttcttagtaa gcctggaagc taatcactaa gagtatcacc agtatgatga cgtgcttcat
    76081 cataaccctt tccttattaa aagccctctt ctccgggaga gggtttcccg tttcagcgtc
    76141 ccgctgaaat cgtcggctta cctcctttcg ccctgaatgc agtctatcgc taacgcgtga
    76201 acaaaatagt ttcatctgtg attattgcgt aacgactcgc aaagaatatt tgctggcagg
    76261 atcgcagact acaaagcctg cggattgaca atcttatcgt gaaggcataa tttcaggagt
    76321 gagggtagag cggggtttcc cccgccctgg tagtcttagt aagcggggaa gcttatgact
    76381 aagagcacca cgatgatgag tagcttcatc atgacccttt ccttattaag gccccttcct
    76441 cgggaggggc tttcccgttt cagcgtcccg ctgaaatcgt cggcttacct cctttcgccc
    76501 tgtatgcagt ctatcgctga cttgcggata aaatagtttc cagtgtgatt attgcgtaac
    76561 gtctcgcaaa gaatatttgc tggcaggatc gcagactgca aagcctgcag attgacaatc
    76621 ttatcgtgaa ggcatacttt caggagtgag ggtagagcgg ggtttccccc gccctggtag
    76681 ccttagtaag ctggccagct aatgactaag agcaccacga tgatgagtag cttcatcatg
    76741 accctttcct tatttatggc cccttcctcg ggaggggctt tcccgtttca gcgtcccgct
    76801 gaaatcggcg gcttacctcc tttcgccatg caagcagtct atcgctaacg cgtagataaa
    76861 atagtttcct gtgttattac tggatgcgtg ctcgcaaatg tgcccgtcat tcagacgatt
    76921 cccgacagtg tttcataatt cctccatttt tctcccttat tggctggcta cactagtatc
    76981 attccgcgaa acgtttcagg aagagaaact cttaacgatg aaaggcagtt ataaatcccg
    77041 ttgggtaatc gtggtggtta tcgccgccat cgccgcattc tggttctggc aaggccgcaa
    77101 tgactcccag agtgcagccc caggggcgac gaaacaagcg cagcaatcgc cagcgggtgg
    77161 tcgccgtggt atgcgttccg gcccattagc cccggttcag gcggcgaccg ccgtagaaca
    77221 ggcagttccg cgttacctca ccgggcttgg caccattatc gccgctaata ctgttacggt
    77281 gcgcagccgc gtggacggtc aactgatggc gttacatttc caggaaggcc agcaggtcaa
    77341 agcaggcgat ttactggcag aaattgaccc cagccagttc aaagttgcat tagcacaaac
    77401 ccagggccaa ctggcaaaag ataaagccac gcttgctaac gcccgccgtg atctggcgcg
    77461 ttatcagcaa ctggcaaaaa ccaatctcgt ctcccgtcag gagctggatg cccaacaggc
    77521 gctggtcagt gaaaccgaag gcaccattaa ggctgatgaa gcaagcgtcg ccagcgcgca
    77581 gttgcaactc gactggagcc gcatcaccgc accagtcgat ggtcgcgttg gtctcaagca
    77641 ggttgatgtt ggtaaccaaa tctccagtgg tgataccacc ggaattgtgg tgatcaccca
    77701 gacgcatcct atcgatttag tctttaccct gccggaaagc gatatcgcta ctgtagtgca
    77761 ggcgcaaaaa gccggaaaac cgctggtggt agaagcctgg gatcgcacca attcgaagaa
    77821 attaagtgaa ggcacgctgt taagcctcga taaccaaatc gatgccacaa ccggtacgat
    77881 taaagtgaaa gcacgcttta ataatcagga tgatgcgctg tttcccaatc agtttgttaa
    77941 cgcgcgcatg ttagtcgaca ccgaacaaaa cgccgtagtg atcccaacag ccgccctgca
    78001 aatgggcaat gaaggccatt ttgtctgggt gctgaatagc gaaaacaagg tcagcaaaca
    78061 tctggtgacg ccgggcattc aggacagtca gaaagtggtg atccgtgcag gtatttctgc
    78121 gggcgatcgc gtggtgacag acggcattga tcgcctgacc gaaggggcga aagtggaagt
    78181 ggtggaagcc cagagcgcca ccactccgga agagaaagcc accagccgcg aatacgcgaa
    78241 aaaaggagca cgctcctgat gcaggtgtta cccccgagca gcacaggcgg cccgtcgcgc
    78301 ctgtttatta tgcgtcctgt cgccaccacg ctgctgatgg tggcgatctt actcgccggg
    78361 attatcggtt atcgcgccct gcccgtttcg gcgctgccgg aagtggacta tccgaccatt
    78421 caggtggtca cgctctaccc aggtgccagc ccggatgtca tgacctctgc cgttaccgcg
    78481 ccgctcgaac gccagttcgg gcagatgtct ggcctgaaac agatgtcgtc gcaaagttcc
    78541 ggcggtgcgt cagttatcac tttgcagttc cagctaacat taccgctcga tgtcgccgag
    78601 caggaagtgc aggccgcgat taacgctgcg accaacttgt tgccgagcga tctgcctaac
    78661 ccgccggttt acagcaaagt gaacccggca gatccgccga tcatgacgct cgccgtcacc
    78721 tcaaccgcca tgccgatgac gcaagtggaa gatatggtgg aaacccgcgt cgcgcagaaa
    78781 atctcgcaga tttccggcgt cggcctggtg acgctttccg gcggtcagcg tccggctgtt
    78841 cgcgtcaaac ttaacgctca ggcgattgcc gccctcggcc tgaccagcga aaccgtgcgc
    78901 accgccatta ccggcgctaa cgttaactcg gcaaaaggta gcctcgacgg cccttcccgt
    78961 gcagtcacgc tttccgcgaa cgaccagatg caatccgccg aagagtatcg ccagctaatc
    79021 atcgcctacc agaacggcgc gccaattcgt ctgggcgatg tcgcaactgt agagcaaggt
    79081 gcagaaaaca gctggctcgg cgcgtgggcg aacaaagaac aggccattgt gatgaatgtt
    79141 cagcgccagc ccggtgctaa cattatctcc accgccgaca gcattcggca gatgctgcca
    79201 cagctcactg agagtctgcc gaaatcggtg aaggtgacag tgctttccga tcgcaccacc
    79261 aatatccgcg catccgtcga tgatactcag tttgaattga tgatggctat cgcgctggta
    79321 gtcatgatta tctacctgtt tttgcgcaat attccggcga ccatcattcc cggtgttgct
    79381 gtaccgctgt cgttaatcgg cactttcgcg gttatggtgt ttctcgattt ttcaatcaat
    79441 aacctgacac tgatggcgtt aactatcgcc accggattcg tggtcgatga cgccatcgtg
    79501 gtgatcgaaa acatttcccg ctatatcgaa aaaggcgaaa aaccgttggc ggcggcgctc
    79561 aagggcgcag gtgaaatcgg ctttaccatt atctcgctga ccttctcact gattgcggtg
    79621 ttgatcccac tgctgtttat gggcgatatc gtcgggcgac tgttccgcga atttgctatt
    79681 accctggcgg tagcgatttt gatctcagcg gtggtgtcgc tgaccctgac accgatgatg
    79741 tgcgcgcgga tgctcagcca ggagtcgttg cgtaaacaga accgcttctc ccgtgcctcg
    79801 gaaaaaatgt tcgacaggat aatcgccgcc tatggtcgtg gactggcgaa agtgctgaat
    79861 catccgtggc tgaccttaag cgtggcactc agcacgctgc tgcttagcgt gctgctgtgg
    79921 gtgttcattc cgaaaggttt cttcccggta caggacaatg gcattattca gggcactttg
    79981 caggcaccgc aatccagctc ctttaccaat atggcccagc gacaacgcca ggtcgcggac
    80041 gtgattttgc aggatccggc agtgcaaagc ctgacctcat ttgttggcgt tgatggcact
    80101 aacccgtcgc tgaatagtgc acgtttacaa atcaacctca aaccgttgga tgaacgtgat
    80161 gatcgggtgc aaaaagtcat cgcccgtctg caaacggcgg tagataaagt gccgggcgtc
    80221 gatctcttcc tgcaaccaac gcaggatctg actattgata ctcaggtcag ccgcacccag
    80281 taccagttta ccttgcaggc cacctcactg gatgcgctca gtacctgggt gccacagttg
    80341 atggaaaaac tccagcaact gccacagctt tctgatgtct ccagcgactg gcaggaccaa
    80401 gggctggtgg cgtatgtcaa tgttgatcgc gacagcgcca gccgtctggg gatcagcatg
    80461 gcggatgtcg ataacgccct gtacaacgcg tttggtcagc gactgatttc cactatttat
    80521 actcaggcta accagtatcg cgtagtgctg gagcacaaca ccgaaattac cccgggcctc
    80581 gcggcgctgg ataccattcg cctgaccagt agcgacggcg gcgtggtgcc gttaagctca
    80641 attgccaaag ttgaacagcg ttttgcgccg ctttccatca accatctgga tcagttcccg
    80701 gtaacgacca tctcctttaa cgtgccggat aactattcgc tgggcgatgc ggtgcaggag
    80761 attatggaca ccgaaaagac gctgaatctg ccggtggata tcaccacgca attccagggc
    80821 agcaccctcg ccttccagtc ggcgctgggc agcactgtct ggctgattgt cgcggcggtg
    80881 gtggcgatgt atatcgtgct cggcattctg tacgagagct ttattcaccc gatcaccatt
    80941 ctctcgacgc tacccaccgc aggggtcggc gcgctgctgg cattaatgat tgccggtagc
    81001 gaactggatg tgattgcgat tatcggcatt attttgctga tcggtattgt gaagaagaac
    81061 gccatcatga tgatcgactt cgcgctggct gctgagcgcg agcaaggcat gtcgccgcgc
    81121 gatgcaatct accaggcttg tctgttgcgt tttcgtccga tcctgatgac caccctggcg
    81181 gctctgcttg gcgcgctgcc gctgatgttg agtaccgggg tcggcgcgga actgcgtcgt
    81241 ccgttaggta tcggcatggt cggcggtctg attgtcagcc aggtgctgac gctgttcacc
    81301 acgccggtga tttatctgct gttcgaccgc ctggcattgt ggaccaaaag tcgctttgct
    81361 cgccatgaag aggaggcgta agtgaagttt tttgccctct tcatttaccg cccggtggcg
    81421 acgattttac tgtcggtcgc cattaccctg tgcggcatac tgggcttccg tatgctgccg
    81481 gtcgccccgc tgccgcaggt cgattttccg gtgattatgg tcagcgcctc gctgcccggt
    81541 gcgtcaccag aaacaatggc gtcttccgtt gccacgccgc tggagcgctc gcttgggcgc
    81601 attgccgggg tcagcgaaat gacctccagc agttcgctcg gcagcacacg tattattttg
    81661 cagtttgatt ttgaccggga catcaacggc gcagcgcgtg atgtgcaggc ggcgatcaac
    81721 gctgcacaaa gtttgctgcc cagtgggatg cccagccgtc cgacctatcg caaagcgaac
    81781 ccgtcggatg cgccaattat gatcctcacg ctgacatccg atacttattc gcagggtgaa
    81841 ctgtacgatt tcgcctcgac gcagctggct ccgacgattt cgcaaatcga cggtgttggt
    81901 gatgtcgatg tcggaggcag ctcactgccc gccgtacgcg tcgggctgaa tccgcaggcg
    81961 ctgtttaatc agggcgtgtc gctggacgac gtacgcaccg ccatcagcaa tgccaacgtg
    82021 cgtaaaccgc agggcgcgct ggaagatggc actcaccgct ggcagatcca gaccaatgat
    82081 gagctaaaaa ccgccgctga atatcagccg ttgattattc actacaacaa cggcggcgcg
    82141 gttcgtctgg gcgatgtggc gacggtgacc gactcagtgc aggatgtgcg caacgccggg
    82201 atgaccaacg ccaaaccggc tattttactg atgatccgca aactgccgga agccaatatt
    82261 atccagacgg ttgacagcat ccgggcaaaa ttaccggagt tgcaggaaac cattccggcg
    82321 gcgattgatc tgcaaattgc ccaggatcgc tcccccacca ttcgcgcctc gctggaagaa
    82381 gtcgagcaaa cgctgattat ctcggtggcg ctggtgattc tggtggtgtt tttattcctg
    82441 cgctcgggtc gcgccactat tattcccgcc gtttcggtgc cggtttcgct gattggtacg
    82501 tttgcggcga tgtacctgtg cggattcagt ctcaataacc tttcgttaat ggcgctcacc
    82561 atcgctactg gtttcgtggt ggatgacgcc atcgtggtgc tggaaaacat tgcacgtcat
    82621 ctggaagcgg gaatgaaacc gttgcaagcc gcactgcaag gtactcgcga agtcggtttt
    82681 acggtgctgt cgatgagtct gtcactggtg gcggtgttcc tgccgctgct gttgatgggc
    82741 ggattgccgg gccgactgtt acgcgagttt gccgtgacgc tttctgtcgc cattggtatt
    82801 tcgttgctgg tttctctgac attaacgcca atgatgtgtg gctggatgct gaaagccagc
    82861 aagccgcgcg agcaaaagcg actgcgtggt tttggtcgca tgttggtagc cctgcaacaa
    82921 ggctacggca agtcactgaa atgggtgctc aatcataccc gtctggtggg cgtggtgctg
    82981 cttggcacca ttgcgctgaa tatctggctg tatatctcga tcccgaaaac cttcttcccg
    83041 gagcaggaca ctggcgtgtt gatgggcggg attcaggcgg atcagagtat ttcgtttcag
    83101 gcgatgcgcg gtaagttgca ggatttcatg aaaattatcc gtgacgatcc ggcagtggat
    83161 aatgtcaccg gctttacagg cggttcgcga gtgaacagcg ggatgatgtt tatcaccctc
    83221 aagccacgcg acgaacgcag cgaaacggcg cagcaaatta tcgaccgtct gcgcgtaaaa
    83281 ctggcgaaag aaccgggggc gaatctgttc ctgatggcgg tacaggatat tcgcgttggt
    83341 gggcgtcagt cgaacgccag ctaccagtac acgttgttat ccgacgacct ggcggcactg
    83401 cgcgaatggg agccgaaaat ccgcaaaaaa ctggcgacgt tgccggaact ggcggacgtg
    83461 aactccgatc agcaggataa cggcgcggag atgaatctgg tttacgaccg cgacaccatg
    83521 gcacggctgg gaatcgacgt gcaggccgcc aacagcctgt taaataacgc cttcggtcag
    83581 cggcaaatct cgaccattta ccagccgatg aaccagtata aagtggtgat ggaagtggat
    83641 ccgcgctata cccaggacat cagtgcgctg gaaaaaatgt tcgttatcaa taacgaaggc
    83701 aaagcgatcc cgctgtcgta tttcgctaaa tggcaaccgg cgaacgctcc gctgtcggtg
    83761 aatcatcagg gattatcggc ggcctcgacc atctcgttca acctgccgac cggaaaatcg
    83821 ctctcggacg ccagtgcggc gatcgatcgc gcaatgaccc agcttggtgt gccttcgacg
    83881 gtgcgcggca gttttgccgg aacagcgcag gtgttccagg agacgatgaa ctcacaggtg
    83941 atcctgatta tcgccgccat cgccacggtg tatatcgtgc tgggtatcct ttacgagagt
    84001 tacgtacacc cactgacgat tctctccacc ctgccctcgg cgggcgttgg tgcgctgctg
    84061 gcgctggagc tgttcaatgc cccgttcagc ctaatagccc tgatagggat catgctatta
    84121 atcggcatcg taaagaaaaa cgccattatg atggtcgatt ttgcgctcga agcccaacgg
    84181 cacggtaacc tgacgccgca ggaagctatt ttccaggcct gtctgctgcg ttttcgcccg
    84241 attatgatga ctaccctggc ggcgctgttt ggcgcgctgc cgctggtgtt gtcgggcggt
    84301 gacggctcgg agctgcggca ccccctgggg atcaccattg tcggtggact ggtaatgagc
    84361 cagcttctga cgctgtatac cacgccggtg gtgtatctct ttttcgaccg tctgcggctg
    84421 cgtttttcgc gtaaacctaa acaaacggta accgagtaaa tgacagaact tcccgacagc
    84481 acccgctggc aattgtggat tgtggctttc ggcttcttta tgcagtcgct ggacaccacc
    84541 atcgttaaca ccgcccttcc ctcaatggcg caaagcctcg gggaaagtcc gttgcatatg
    84601 cacatggtca ttgtctctta tgtgctgacc gtggcggtga tgctgcccgc cagcggctgg
    84661 ctggcggaca aagtcggcgt gcgcaatatt ttctttaccg ccatcgtgct gtttactctc
    84721 ggttcactgt tttgcgcgct ttccggcacg ctgaacgaac tgttgctggc acgcgcgtta
    84781 cagggcgttg gcggtgcgat gatggtgccg gtcggcaggt tgacggtgat gaaaatcgta
    84841 ccgcgcgagc aatatatggc ggcgatgacc tttgtcacgt tacccggtca ggtcggtccg
    84901 ctgctcggtc cggcgctcgg cggtctgctg gtggagtacg cgtcgtggca ctggatcttt
    84961 ttgatcaaca ttccggtggg gattatcggt gcgatcgcca cattgctgtt aatgccgaac
    85021 tacaccatgc agacgcggcg ctttcatctt tccggctttt tattgctggc ggttggcatg
    85081 gctgtattaa ccctggcgct ggacggcagt aaaggtacag gtttatcgcc gctggcgatt
    85141 gctggcctgg tcgcagttgg cgtggtggca ctggtgcttt atctgctgca cgccagaaat
    85201 aacaaccgcg ccctgttcag tctgaaactg ttccgcactc gtaccttttc gctgggcctg
    85261 gcggggagct ttgccggacg tattggcagt ggcatgttgc cctttatgac accggttttc
    85321 ctgcaaattg gcctcggttt ctcgccgttt catgccggac tgatgatgat cccgatggta
    85381 cttggcagca tgggaatgaa gcgaattgtg gttcaggtgg tgaatcgctt tggttatcgt
    85441 cgggtactgg tagcgaccac gctgggtctg tcgctgatca ccctgttgtt tatgactacc
    85501 gctctgttgg gctggtacta cgttttgccg ttcgtcctgt ttttacaagg gatggtcaac
    85561 tcgacccgtt tctcctccat gaacaccctg acgctgaaag atctcccgga caatctggcg
    85621 agcagcggca acagtctgct gtcgatgatt atgcaattgt cgatgagtat cggcgtcacc
    85681 atcgccgggc tgttgctggg actttttggt tcgcagcatg tcagcgtcga cagcggcacc
    85741 acacaaaccg tctttatgta cacctggctt agcatggcgt ttatcatcgc ccttccggcg
    85801 ttcatctttg ccagagtgcc gaacgatacg catcaaaatg tagctatttc gcggcgaaaa
    85861 aggagcgcgc aatgaagttc tggcgacctg gtattaccgg caaactgttt ctggcgattt
    85921 tcgccacctg cattgttttg ctgatcagta tgcactgggc ggtgcgtatc agttttgagc
    85981 gtggctttat tgattacatc aagcatggta atgaacagcg attacaactg ttaagtgatg
    86041 cgcttggcga gcagtatgcg cagcatggca actggcgctt cctgcgcaac aatgatcgct
    86101 ttgtctttca gatcctgcgt tcatttgaac acgataattc ggaagataaa cccggcccgg
    86161 gtatgccacc gcacggctgg cgtacccagt tctgggtggt tgatcaaaac aacaaagtgc
    86221 tggttggtcc gcgagcgccg attccacctg acggtacacg gcgacccatt ctggtcaacg
    86281 gtgcggaagt tggcgcggtg atcgcctccc ccgttgagcg gttaacgcgc aatactgata
    86341 tcaatttcga taaacaacag cggcaaacca gctggttgat tgtcgccctg gcaacgttac
    86401 tcgcggcact tgccactttt ctgctggcgc gcggtttact ggcaccggta aaacgacttg
    86461 tcgatggcac gcacaaactg gcggcgggcg atttcactac ccgcgtaacg cccaccagtg
    86521 aagatgaact gggcaaactg gcgcaagact tcaaccagct tgccagcaca ctggagaaaa
    86581 accagcaaat gcggcgcgat tttatggccg atatttctca cgaactgcgt acgccattag
    86641 cggtgctgcg cggtgaactg gaagctattc aggatggcgt gcgcaaattc acgccggaga
    86701 cggtggcgtc tttacaggcg gaggtcggta cactgaccaa actggttgac gatctccatc
    86761 agttgtcgat gtctgatgaa ggcgccctcg cctatcaaaa agcaccggta gatttgatcc
    86821 cactgctgga agtggcgggc ggcgcatttc gcgaacgatt cgccagccgt ggcctgaaac
    86881 tgcaattttc cctgccagac agtattaccg tatttggcga tcgcgaccgt ttaatgcagt
    86941 tattcaataa tttacttgaa aacagcctgc gctacactga cagcggcggc agcctgaaaa
    87001 tctctgccga gcagcacgac aaaacggtgc gcctgacctt tgccgacagt gcgccaggtg
    87061 tcagtgacga gcagctacaa aaattatttg aacgttttta tcgcaccgaa ggttcccgca
    87121 accgcgccag cggtggttcc gggctggggc tggcgatttg cctgaacatt gttgaagcac
    87181 ataatggccg cattattgct gcccattcgc cttttggcgg ggtaagcatt acagtagagt
    87241 taccgctgga acgggattta cagagagaag tatgaccgag ttaccaatcg acgaaaacac
    87301 accgcgtatt ttgatcgtgg aagatgaacc gaagctgggg cagttgctca ttgattatct
    87361 gcgtgctgcg agctatgcgc cgacgcttat cagccacggc gatcaggtac tgccgtatgt
    87421 gcgccagaca ccaccggatc tgatcctgtt ggatctgatg ctccctggca ccgatggctt
    87481 gacgctgtgc cgggaaattc gtcgtttttc tgacattcct atcgtgatgg tgacggcaaa
    87541 aatcgaagag atcgaccgcc tgctcggact ggagattggc gcagatgact acatctgcaa
    87601 gccgtacagc ccacgggaag tggtagcgcg cgtcaaaacc attttgcgcc gttgcaaacc
    87661 gcagcgcgaa ttgcagcaac aggatgccga aagcccgttg attatcgacg aaggtcgttt
    87721 tcaggcttca tggcgcggta aaatgcttga cctgacgcct gcggaatttc gtctgttgaa
    87781 aacgctctct catgaaccag gaaaagtgtt ctcccgcgag caattgctca atcatcttta
    87841 tgacgactac cgcgtagtaa ccgaccgcac cattgacagc cacattaaaa acctgcgccg
    87901 caagctggaa tctctcgacg ccgaacagtc atttatccgc gccgtttacg gcgttggtta
    87961 ccgctgggaa gccgacgcct gccgcatcgt ttagttttag cgacatgact ttgttagccg
    88021 gagatggcgt tcagaccaga tccggcaaca ttatcccacg catggtcagc aaactg
//
LOCUS       DAESHI010000002        81388 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.002, whole genome shotgun sequence.
ACCESSION   DAESHI010000002 DAESHI010000000
VERSION     DAESHI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 81388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81388
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.002"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..675)
                     /locus_tag="KW011_000089"
     CDS             complement(<1..675)
                     /locus_tag="KW011_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HBH7658536.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHRE"
     gene            complement(835..1047)
                     /gene="rpmE"
                     /locus_tag="KW011_000090"
     CDS             complement(835..1047)
                     /gene="rpmE"
                     /locus_tag="KW011_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBH7658537.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            1250..3448
                     /gene="priA"
                     /locus_tag="KW011_000091"
     CDS             1250..3448
                     /gene="priA"
                     /locus_tag="KW011_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBH7658538.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3604..4629
                     /gene="cytR"
                     /locus_tag="KW011_000092"
     CDS             3604..4629
                     /gene="cytR"
                     /locus_tag="KW011_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HBH7658539.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4721..5680
                     /gene="ftsN"
                     /locus_tag="KW011_000093"
     CDS             4721..5680
                     /gene="ftsN"
                     /locus_tag="KW011_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HBH7658540.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5773..6303
                     /gene="hslV"
                     /locus_tag="KW011_000094"
     CDS             5773..6303
                     /gene="hslV"
                     /locus_tag="KW011_000094"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HBH7658541.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            6313..7644
                     /gene="hslU"
                     /locus_tag="KW011_000095"
     CDS             6313..7644
                     /gene="hslU"
                     /locus_tag="KW011_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HBH7658542.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7711..8637
                     /gene="menA"
                     /locus_tag="KW011_000096"
     CDS             7711..8637
                     /gene="menA"
                     /locus_tag="KW011_000096"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBH7658543.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8730..9215
                     /gene="rraA"
                     /locus_tag="KW011_000097"
     CDS             8730..9215
                     /gene="rraA"
                     /locus_tag="KW011_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HBH7658544.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(9300..9545)
                     /gene="zapB"
                     /locus_tag="KW011_000098"
     CDS             complement(9300..9545)
                     /gene="zapB"
                     /locus_tag="KW011_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HBH7658545.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9971..10816
                     /gene="glpF"
                     /locus_tag="KW011_000099"
     CDS             9971..10816
                     /gene="glpF"
                     /locus_tag="KW011_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HBH7658546.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10839..12350
                     /gene="glpK"
                     /locus_tag="KW011_000100"
     CDS             10839..12350
                     /gene="glpK"
                     /locus_tag="KW011_000100"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBH7658547.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            12480..13490
                     /gene="glpX"
                     /locus_tag="KW011_000101"
     CDS             12480..13490
                     /gene="glpX"
                     /locus_tag="KW011_000101"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBH7658548.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13587..14333
                     /gene="fpr"
                     /locus_tag="KW011_000102"
     CDS             13587..14333
                     /gene="fpr"
                     /locus_tag="KW011_000102"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HBH7658549.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(14338..14766)
                     /gene="uspD"
                     /locus_tag="KW011_000103"
     CDS             complement(14338..14766)
                     /gene="uspD"
                     /locus_tag="KW011_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HBH7658550.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14793..15092)
                     /locus_tag="KW011_000104"
     CDS             complement(14793..15092)
                     /locus_tag="KW011_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HBH7658551.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(15304..15744)
                     /locus_tag="KW011_000105"
     CDS             complement(15304..15744)
                     /locus_tag="KW011_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBH7658552.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15845..16444
                     /locus_tag="KW011_000106"
     CDS             15845..16444
                     /locus_tag="KW011_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HBH7658553.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16552..17319
                     /gene="tpiA"
                     /locus_tag="KW011_000107"
     CDS             16552..17319
                     /gene="tpiA"
                     /locus_tag="KW011_000107"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBH7658554.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(17374..18129)
                     /gene="cdh"
                     /locus_tag="KW011_000108"
     CDS             complement(17374..18129)
                     /gene="cdh"
                     /locus_tag="KW011_000108"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HBH7658555.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(18236..19225)
                     /gene="sbp"
                     /locus_tag="KW011_000109"
     CDS             complement(18236..19225)
                     /gene="sbp"
                     /locus_tag="KW011_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HBH7658556.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19545..20507)
                     /gene="pfkA"
                     /locus_tag="KW011_000110"
     CDS             complement(19545..20507)
                     /gene="pfkA"
                     /locus_tag="KW011_000110"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBH7658557.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20688..21590)
                     /gene="fieF"
                     /locus_tag="KW011_000111"
     CDS             complement(20688..21590)
                     /gene="fieF"
                     /locus_tag="KW011_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HBH7658558.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21825..22043)
                     /gene="ogrK"
                     /locus_tag="KW011_000112"
     CDS             complement(21825..22043)
                     /gene="ogrK"
                     /locus_tag="KW011_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HBH7658559.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(22125..23288)
                     /locus_tag="KW011_000113"
     CDS             complement(22125..23288)
                     /locus_tag="KW011_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HBH7658560.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDITLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQITITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHCLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            complement(23288..23767)
                     /locus_tag="KW011_000114"
     CDS             complement(23288..23767)
                     /locus_tag="KW011_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH7658561.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISKVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATKFTGMTGAG"
     gene            complement(23782..26229)
                     /locus_tag="KW011_000115"
     CDS             complement(23782..26229)
                     /locus_tag="KW011_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBH7658562.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRRASKLLSGDIRETQKSLRE
                     LNGHASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTIAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSAEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPVFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTAASTGKLDKLTAAFKASDGKTAELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRKLTQTATKYVLKLDGWVQKNKSLASTIGLIAGGALALTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFKNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(26222..26341)
                     /locus_tag="KW011_000116"
     CDS             complement(26222..26341)
                     /locus_tag="KW011_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HBH7658563.1"
                     /translation="MADVAVIFHWPPSELYPMSLTELITWREKALRRSGNTNE"
     gene            complement(26374..26649)
                     /locus_tag="KW011_000117"
     CDS             complement(26374..26649)
                     /locus_tag="KW011_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HBH7658564.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPSAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(26706..27224)
                     /locus_tag="KW011_000118"
     CDS             complement(26706..27224)
                     /locus_tag="KW011_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HBH7658565.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(27237..28427)
                     /locus_tag="KW011_000119"
     CDS             complement(27237..28427)
                     /locus_tag="KW011_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HBH7658566.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTVSEVKAYRQNFSQRELMVIWPDFLAWDTVASTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTS
                     VNSN"
     gene            28838..30256
                     /locus_tag="KW011_000120"
     CDS             28838..30256
                     /locus_tag="KW011_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7658567.1"
                     /translation="MTYALIDNSTLTAVQRIEGKVKTRSKDSVDTDIIAFENYIQSIL
                     FYDHVIAVDDYIPEYRGSRISSFREILFLDKAQYGLDEIESEAKKISDALKPKIRGGT
                     FVNDDFKKLIELLQTHIICTWDISSSVYHLTLKNLSDGGKEFEKYGNIAAAIFSELRD
                     AAESGNRTNGDVELLDRFGNPIRKGYKVPGAKWGDGTSAGEASDAIKAFVASLVWLAN
                     RAVFYSLTAKYLQADTFLYPLRQAYQQTYLSQNCQYGFDYAKHIVENFSTSLSHDLID
                     IHSGGKAVATATSLPLFSAWLAKETGDPSCIVEAAYNIRDNNEFVRAREKMREIKRLF
                     DENELPQANKSVRALLTEVSKESDYIRTKYGLKTRQGVPVTKLVHVYNTVAAINSLPK
                     LPTFNFKVKVPDFLYDLKKPKGFRALYRNLTNDLSTVWSLGEARDILGSKVVIDNHAR
                     IYSPKHEEPRFKNSHSPYKSPM"
     gene            complement(30371..30898)
                     /locus_tag="KW011_000121"
     CDS             complement(30371..30898)
                     /locus_tag="KW011_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBH7658568.1"
                     /translation="MQHLINITAGNPKTVEQYQLTKDFDVVWFFSEDGKNWYEEQKYF
                     ADDTIKIAYDKDNIIRYVEKDVTAIRPDGLSVVEVPDITANRRADISGNWMFKDCTVI
                     KRIYTAEELQQQAENRKARLLADAESVILPLERAVRLNMATDEERSRLEAWERYSVLV
                     SRVDPANPEWPEMPQ"
     gene            complement(30902..33220)
                     /locus_tag="KW011_000122"
     CDS             complement(30902..33220)
                     /locus_tag="KW011_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HBH7658569.1"
                     /translation="MSTKFRTVITTAGAAKLAAATAPGGRKVNITTMAVGDGGGKLPV
                     PDAGQTGLIHEVWRHTLNKISQDKRNSNYIIAELVIPPEVGGFWMRELGLYDDAGTLI
                     AVANMAESYKPALAEGSGRSQTCRMVIIVSSVASVELTIDTTTVMATQDYVDDKIAEH
                     EQSRRHPDASLTAKGFTQLSSATNSTSETLAATPKAVKAAYDLANGKYTAQDATTARK
                     GLVQLSSATNSTSETLAATPKAVKTAYDLANGKYTAQDATTARKGLVQLSSATNSDSE
                     TLAATPKAVKTAYDLANGKYTAQDATTARKGLVQLSSAINSDSETLAATPKAVKSAYD
                     NAEKRLQKDQNGADIPGKDTFTKNIGACRAYSGALSTEAGNWTTAQFIEWLDSRGAFN
                     HPYWMCKGSWSYANNKIITDTGCGDIHLAGCVVEVMGTKSAITIRVTTPTTSSGGGTT
                     SAQFTYINHGDGYSPGWRRDWNRQGDSMTGTINQDGGSQNAYMSTALCSGTRGGKKYL
                     RKFRGGEGDTIWHETVQGGVIRWATGNNDAQEELSLSSAYGLRSRGEITSLSANGLRI
                     AYGNYGFFIRNDGGSTYLMLTASGDKFGTWNGLRPLTINNANGGVSMGHGLSVTGDIV
                     SSTKVRAGSGKKFTVSSSNTSTKEAAFNLWGNSSRPVVAELGDDAGWHFYSQRNTDNS
                     ITFAVNGQVSPSNYGNFDSRYVRDIRLGGAATYKPANNGMTWTHQAPSGCVYSGIIVQ
                     DTGSNSADNIGGVYYRPVQKYINGTWYNVAQV"
     gene            complement(33231..33761)
                     /locus_tag="KW011_000123"
     CDS             complement(33231..33761)
                     /locus_tag="KW011_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HBH7658570.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVRIRELWNPDTCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLINVTE
                     WWENSDPPGTFRLDIGVLESGITEAMYQEMERLIADAKPASRHLIGLNITRDIPGYLF
                     AGGVAYDGDVITVYPG"
     gene            complement(33754..34662)
                     /locus_tag="KW011_000124"
     CDS             complement(33754..34662)
                     /locus_tag="KW011_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HBH7658571.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLI
                     ITPADDSTLPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELTAP
                     LADIVLNSTQASFCTEYRVVTGGSDE"
     gene            complement(34667..35014)
                     /locus_tag="KW011_000125"
     CDS             complement(34667..35014)
                     /locus_tag="KW011_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HBH7658572.1"
                     /translation="MTARYLGMNRSDGLTVSDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEARVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(35011..35646)
                     /locus_tag="KW011_000126"
     CDS             complement(35011..35646)
                     /locus_tag="KW011_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HBH7658573.1"
                     /translation="MNTLANIQELARALRNMIRTGIIVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVTVKASTRVTLDTPEVVCT
                     NKLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            complement(35713..36165)
                     /locus_tag="KW011_000127"
     CDS             complement(35713..36165)
                     /locus_tag="KW011_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HBH7658574.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSAELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEETRKDGKKIHYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            complement(36158..36625)
                     /locus_tag="KW011_000128"
     CDS             complement(36158..36625)
                     /locus_tag="KW011_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH7658575.1"
                     /translation="MLKPDSLRRALTDAVTVLKTSPEMLRIFVDNGSIASTLATSLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            complement(36588..36746)
                     /locus_tag="KW011_000129"
     CDS             complement(36588..36746)
                     /locus_tag="KW011_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein"
                     /protein_id="HBH7658576.1"
                     /translation="MPGSDPQTNGDLSADIRQLENALARCASQVKMIKHCQDENDAQT
                     RQPAQGAD"
     gene            complement(36733..37158)
                     /locus_tag="KW011_000130"
     CDS             complement(36733..37158)
                     /locus_tag="KW011_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysB family phage lysis regulatory protein"
                     /protein_id="HBH7658577.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLVKHKNASLRALLDRANNVASEQQ
                     TTITMLKNQLHVALTRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHQRTACADASDCPQRLPESEPLPDAGQ"
     gene            complement(37146..37571)
                     /locus_tag="KW011_000131"
     CDS             complement(37146..37571)
                     /locus_tag="KW011_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysA"
                     /protein_id="HBH7658578.1"
                     /translation="MKKLSLSLMLNVSLALMLALSLIYPQSVAVSFVAAWAILATVIC
                     VIAGGVGVYAAEYVLERYGRELPPESLAVKIVTSLFLQPVPWRRRAAALMVMVATFIS
                     LVAAGWIFTALIYLVASLFFRLIRTACRQRLEGREPCQN"
     gene            complement(37586..38083)
                     /locus_tag="KW011_000132"
     CDS             complement(37586..38083)
                     /locus_tag="KW011_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HBH7658579.1"
                     /translation="MPVISTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            complement(38083..38364)
                     /locus_tag="KW011_000133"
     CDS             complement(38083..38364)
                     /locus_tag="KW011_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBH7658580.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            complement(38368..38571)
                     /locus_tag="KW011_000134"
     CDS             complement(38368..38571)
                     /locus_tag="KW011_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HBH7658581.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVEAVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            complement(38571..39080)
                     /locus_tag="KW011_000135"
     CDS             complement(38571..39080)
                     /locus_tag="KW011_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HBH7658582.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFSRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            complement(39180..39923)
                     /locus_tag="KW011_000136"
     CDS             complement(39180..39923)
                     /locus_tag="KW011_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HBH7658583.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDPRLLTDTLELTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALVHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRTPRKTRSVTPAKRGRPKKKAS"
     gene            complement(39927..40145)
                     /locus_tag="KW011_000137"
     CDS             complement(39927..40145)
                     /locus_tag="KW011_000137"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HBH7658584.1"
                     /translation="MLITKLENLSIYYMDDSHRRVIVENPKLDRVENYESMNIDYVVE
                     DYAAGCLVEKIKVGDFSTPAKATAEPGA"
     gene            complement(40082..40999)
                     /locus_tag="KW011_000138"
                     /pseudo
     CDS             complement(40082..40999)
                     /locus_tag="KW011_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228319.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
     gene            complement(41058..41912)
                     /locus_tag="KW011_000139"
     CDS             complement(41058..41912)
                     /locus_tag="KW011_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HBH7658585.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQEVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            42086..43858
                     /locus_tag="KW011_000140"
     CDS             42086..43858
                     /locus_tag="KW011_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HBH7658586.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNIANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKVSVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            43858..44892
                     /locus_tag="KW011_000141"
     CDS             43858..44892
                     /locus_tag="KW011_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBH7658587.1"
                     /translation="MSKKKGKTPRPAAKTITASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
     gene            45264..46406
                     /locus_tag="KW011_000142"
     CDS             45264..46406
                     /locus_tag="KW011_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBH7658588.1"
                     /translation="MDIIDLKSQLENEDNAKKRAELLAQLEVIKQNAEEQIRAEQKDV
                     DFETKEFTVELLVNKYHNGLEDDTNELFVPDYQRDFVWSEKRQSKLIESLILGFPIPY
                     IFIADVLSEDPDLDGRIEIVDGSQRVRTIHAFIQNELVLQDLKSLDVLNGFKFQDLPL
                     SRQRRFMRIPVRVIELSSKCNEETRRDLFERINSGSDILKDMEVRKGSELGSTNLYTQ
                     VIKPCSTIPKFKELAPLSQTKEKRDERLEFTLRFFAYVDKYKNFDHSVRDFLNDYMSE
                     NRDIDETKQNSMKQEFANVLSFVENYFPAGFKKTITAKSTPRVRFESLAVGAALALRE
                     VPDLVPKNLDWLSSEEFKRLTTSDGANSRVKVIERIEYVRDKLLED"
     gene            46408..47088
                     /locus_tag="KW011_000143"
     CDS             46408..47088
                     /locus_tag="KW011_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7658589.1"
                     /translation="MDRFREDFDERSGEILAYLDLLKFIEYAGAELISADDKEHKFSI
                     TAQSRKTLKGAVYILLYNLIESTMREAICLIHETIYDRNVEFDKLRKNLRSEILKRLK
                     NESVNVESLVNHLTKGISCGISYGTFNKKKIFSGNIDREEIKEKSKIYGFSTHSDYTH
                     TKHGEKLATVKQHRNDLSHGNVSFAEIGKNVSYQDLENISLEVIAYLDAIVNNIEHYI
                     NNNEYLEQ"
     gene            complement(47118..48140)
                     /locus_tag="KW011_000144"
     CDS             complement(47118..48140)
                     /locus_tag="KW011_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBH7658590.1"
                     /translation="MTVKVIDLFCGAGGLTHGLQLAGLDVVAGIDLEGECRFPYERNN
                     KSKFIEQDIAKVTKEELLRLYGDASVKVLAGCAPCQPFSKYTQGKDKADDKKWPLLYE
                     FERLIREVSPEIVTMENVPDVTKHKVYNDFYNSLLKLGYYVWASKIDCVEYGIPQNRS
                     RHVLLASKLGAIELVKRDDVILKTVRDAIGNLPPLEDGQTDPNDILHRASKLNPINKK
                     RIIHSVPGGTWKDWPKELVAACHMKSSGKGYASVYGRMSWDKPSPTITTLCYGFGNGR
                     FGHPEQHRAISLREAALLQTFPMDYIFVEDKNKFVIRSIGKMIGNAVPVELGKAIGQS
                     IKNHLE"
     gene            complement(48227..50524)
                     /locus_tag="KW011_000145"
     CDS             complement(48227..50524)
                     /locus_tag="KW011_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HBH7658591.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASMLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELSDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWISQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDSGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDE
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWNDPEVVRALRGALKHGLRRPNRQ
                     QRNGSPLKPHEIAPSARLTRSERMQITRIRVDLAQNGIRPQRWELEVLARGATVNYDG
                     KKFTYPLADEWSGFSTVLESNSVKR"
     gene            complement(50514..50789)
                     /locus_tag="KW011_000146"
     CDS             complement(50514..50789)
                     /locus_tag="KW011_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HBH7658592.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGR"
     gene            complement(50786..51010)
                     /locus_tag="KW011_000147"
     CDS             complement(50786..51010)
                     /locus_tag="KW011_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HBH7658593.1"
                     /translation="MPDNIDFIQEQQAELLERQINAARVKHCGASALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRTA"
     gene            complement(51013..51312)
                     /locus_tag="KW011_000148"
     CDS             complement(51013..51312)
                     /locus_tag="KW011_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HBH7658594.1"
                     /translation="MSCSHSVVLLNNALKIAVMKNGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTELASYCADVSRKLNDKRS"
     gene            complement(51312..51536)
                     /locus_tag="KW011_000149"
     CDS             complement(51312..51536)
                     /locus_tag="KW011_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HBH7658595.1"
                     /translation="MHTVSENQCGKYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(51600..52100)
                     /locus_tag="KW011_000150"
     CDS             complement(51600..52100)
                     /locus_tag="KW011_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HBH7658596.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(52270..52542)
                     /locus_tag="KW011_000151"
     CDS             complement(52270..52542)
                     /locus_tag="KW011_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7658597.1"
                     /translation="MDAENYVIQYPLDAVHVDKFADLLGKPKTAVSEMVKANKLPIIE
                     LRDPCKPKARAGEKWVFIPEFNRAVREAFYNRPVEQRDAWLLWMGL"
     gene            52679..52972
                     /locus_tag="KW011_000152"
     CDS             52679..52972
                     /locus_tag="KW011_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH7658598.1"
                     /translation="MKLSEKIKALREAEGLSQSKFCEIIELPLSTLKKYEGGNFEPGG
                     TALLKITMHPTFQKYALWLMTDKTAPDAGQIAPALAHIGPESTESNHSAKRIG"
     gene            53042..54022
                     /locus_tag="KW011_000153"
     CDS             53042..54022
                     /locus_tag="KW011_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH7658599.1"
                     /translation="MAIKKLDDGRYEVDIRPRGRDGKRIRRKFERKAEALAFERYTIA
                     NASQKEWGGQRADRRTLSELLDIWWKYHGQNHEHGTKEFNHLLKTISGIGDIPVSRMS
                     KRALMDYRSMRLRDGISAATINRDMYRLSGMFTKLIQLDEFSGQHPIHGLPPLAEANP
                     EMTFLEKAEIEKLLNVLAGDDLLVALLCLSTGGRWTKVATLKPAQITNCRVTFLKTKN
                     GKKRTVPISEELEKKVKEEASAKLFKVDYEKFCGILRRVKPDIPPNQATHILRHTFAS
                     HFMMNGGNIIALQQILGHASIQQTMAYAHLAPDYLQNAVALNPLKGGVTL"
     gene            complement(54208..54708)
                     /gene="cpxP"
                     /locus_tag="KW011_000154"
     CDS             complement(54208..54708)
                     /gene="cpxP"
                     /locus_tag="KW011_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HBH7658600.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            54858..55556
                     /gene="cpxR"
                     /locus_tag="KW011_000155"
     CDS             54858..55556
                     /gene="cpxR"
                     /locus_tag="KW011_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HBH7658601.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            55553..56926
                     /gene="cpxA"
                     /locus_tag="KW011_000156"
     CDS             55553..56926
                     /gene="cpxA"
                     /locus_tag="KW011_000156"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HBH7658602.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(57032..57706)
                     /gene="yiiM"
                     /locus_tag="KW011_000157"
     CDS             complement(57032..57706)
                     /gene="yiiM"
                     /locus_tag="KW011_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HBH7658603.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(57855..58838)
                     /gene="kdgT"
                     /locus_tag="KW011_000158"
     CDS             complement(57855..58838)
                     /gene="kdgT"
                     /locus_tag="KW011_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HBH7658604.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(59098..59718)
                     /gene="sodA"
                     /locus_tag="KW011_000159"
     CDS             complement(59098..59718)
                     /gene="sodA"
                     /locus_tag="KW011_000159"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HBH7658605.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            60003..61037
                     /gene="rhaT"
                     /locus_tag="KW011_000160"
     CDS             60003..61037
                     /gene="rhaT"
                     /locus_tag="KW011_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HBH7658606.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(61034..61900)
                     /gene="rhaR"
                     /locus_tag="KW011_000161"
     CDS             complement(61034..61900)
                     /gene="rhaR"
                     /locus_tag="KW011_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HBH7658607.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(61956..62792)
                     /gene="rhaS"
                     /locus_tag="KW011_000162"
     CDS             complement(61956..62792)
                     /gene="rhaS"
                     /locus_tag="KW011_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HBH7658608.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            63080..64549
                     /gene="rhaB"
                     /locus_tag="KW011_000163"
     CDS             63080..64549
                     /gene="rhaB"
                     /locus_tag="KW011_000163"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBH7658609.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            64546..65805
                     /gene="rhaA"
                     /locus_tag="KW011_000164"
     CDS             64546..65805
                     /gene="rhaA"
                     /locus_tag="KW011_000164"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBH7658610.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            66047..66871
                     /gene="rhaD"
                     /locus_tag="KW011_000165"
     CDS             66047..66871
                     /gene="rhaD"
                     /locus_tag="KW011_000165"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBH7658611.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            66881..67195
                     /gene="rhaM"
                     /locus_tag="KW011_000166"
     CDS             66881..67195
                     /gene="rhaM"
                     /locus_tag="KW011_000166"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBH7658612.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            67496..67942
                     /locus_tag="KW011_000167"
     CDS             67496..67942
                     /locus_tag="KW011_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBH7658613.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            67953..69404
                     /locus_tag="KW011_000168"
     CDS             67953..69404
                     /locus_tag="KW011_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HBH7658614.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            69394..70464
                     /locus_tag="KW011_000169"
     CDS             69394..70464
                     /locus_tag="KW011_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBH7658615.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            70464..72212
                     /locus_tag="KW011_000170"
     CDS             70464..72212
                     /locus_tag="KW011_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HBH7658616.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(72262..73302)
                     /locus_tag="KW011_000171"
     CDS             complement(72262..73302)
                     /locus_tag="KW011_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HBH7658617.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFK
                     QGPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLESFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADK
                     GGMNFSFINSADQYQLETKKYVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALRE
                     YNEMVDDYNSLR"
     gene            complement(73455..74288)
                     /gene="fdhD"
                     /locus_tag="KW011_000172"
     CDS             complement(73455..74288)
                     /gene="fdhD"
                     /locus_tag="KW011_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBH7658618.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            74482..77532
                     /gene="fdnG"
                     /locus_tag="KW011_000173"
     CDS             74482..77532
                     /gene="fdnG"
                     /locus_tag="KW011_000173"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:75067..75069,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBH7658619.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            77545..78447
                     /gene="fdoH"
                     /locus_tag="KW011_000174"
     CDS             77545..78447
                     /gene="fdoH"
                     /locus_tag="KW011_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HBH7658620.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            78444..79079
                     /gene="fdoI"
                     /locus_tag="KW011_000175"
     CDS             78444..79079
                     /gene="fdoI"
                     /locus_tag="KW011_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBH7658621.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            79076..80005
                     /gene="fdhE"
                     /locus_tag="KW011_000176"
     CDS             79076..80005
                     /gene="fdhE"
                     /locus_tag="KW011_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HBH7658622.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(80335..80577)
                     /locus_tag="KW011_000177"
     CDS             complement(80335..80577)
                     /locus_tag="KW011_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7658623.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
BASE COUNT        19640 a        21437 c        20642 g        19669 t
ORIGIN      
        1 ttcgcggtgg aacgtctgtg tgcgcccgaa gcggtccacc agcccggtca gtacccggta
       61 cggcggcagc ggcgcaggca gcacctcatc cgcttccggc acccgctcac accagccgag
      121 cagccaccac ggcccctgcg gactgtttgt cgccagataa cgatgcggac ttaagcggag
      181 ttcttccggc agcgcctgcc agagtgcggc cagccggtga ccttcatcca gtttcgccac
      241 gccgccgcgc accagccaga gtgattcgct gcggctgtaa ccgtcctcac cgggaaacag
      301 gtgctcaaaa tacaggcttc tgccgccgtt atcactgagt atcagtgtgt tatcgcgcag
      361 ctgtaagcgg atatccgcag gcattttcca gccggggccg aggctcccca ccggcgcggg
      421 cgtttttgtc cggtaactgc tgtaggtgcg ggagaggatg aacggcagcg gggcgggcag
      481 ggcgatgtcg gtttcaccgg gaaggacctt cgcaccgagc agcggattga ccggatggcc
      541 ggacgtcacc ccgccggggc acaccgaaca ggccacgccg gtgggggcac cgatgcgcac
      601 cccggctgaa ccctggacaa tgctaccgcc atactgcgtc atgtcgccct gacgcgccgc
      661 cggttttccg ctcatcgtaa aatccttttg aaataaataa gaaccagaag cctagcgcca
      721 gtcaacaaca ggaaatgata atgagttaac accccggaat ttttcagaat ttataaaagg
      781 caaattacag gcacaaaaaa agcgccgtgc ggcgcttttt tcggaaatcc ggtcttattt
      841 gctgcccggg atgttgaaac gcttgttgaa gcggtcaaca cggccaccgg tagcaacatc
      901 acgctgtttg ccagtgaaga acgggtggca cttgctgcac acgtcgaggt tcaggtcatg
      961 accaacggtg gagcggattt tcattacgtt accgcaagag cagctagcag taatttcttc
     1021 gtatttcggg tgaatatctt ttttcatggg aaaacctcgg tttaaggccg cgtcgctctt
     1081 ccagccctaa cgccagacac cacgcgatgt taaaagtata gcttcaatac gatcatttcg
     1141 tacgaaggcg cgaaatcata cagaaattaa ccagcgtatg caaactgatc cgcactcttc
     1201 tacggcaatg tgtatactaa cccaccgaat ttcaagtcag gatgatgcta tgcccgttgc
     1261 ccacgttgcc ttgcccgttc cgcttcctcg tacctttgac tatctgctgc cagaaggcat
     1321 ggcggttaaa gccgggtgtc gcgtgcgcgt gccgtttggc aaacagcagg agcgcatcgg
     1381 ggttgtggta tcagttagcg atgtcagtga gctgccgctt aacgagctga aagcggtagt
     1441 tgaagtgctg gacgttgagc cggtatttac tcactccgtc tggcgattac tgctctgggc
     1501 ggcagattac tatcatcatc cgattggcga tgtgctgttt catgccttgc cgattttact
     1561 gcgccagggg cggcctgcgg cgaacgcgcc gatgtggtac tggtttgcca ctgaacaagg
     1621 ccaggcggtg gatctgaaca gcctgaaacg ctcacccaag caacaacagg cgctggcggc
     1681 gttacggcaa gggaaaatct ggcgcgacca ggttgccacg ctcgaattta atgatgccgc
     1741 gttgcagacg ctgcgcaaaa aaggtctgtg tgatttagca agtgaaacac cagagtttag
     1801 cgactggcga acgaactatg ccgtttctgg tgagcggttg cgattgaata ccgaacaggc
     1861 caccgccgtt ggcgcaattc atagcgcggc agatactttt tctgcctggc tgctggcggg
     1921 cgttaccggt tccggtaaaa cggaggttta tctcagtgta ctggaaaacg tgctcgctca
     1981 gggcaaacag gcgctggtga tggtgccgga aatcggcctg acaccgcaaa ctatcgcccg
     2041 ttttcgtgaa cggtttaatg cgcccgtgga agttctgcat tccggtctga acgacagcga
     2101 gcgtctttcg gcgtggctga aagcgaaaaa tggcgaggcg gcgattgtga tcggcacccg
     2161 ctccgcgctg tttacgccgt ttaaaaatct cggcgtgatt gtcattgacg aagagcacga
     2221 cagttcctac aagcagcagg aaggctggcg ctatcatgcc cgcgacctgg cggtgtatcg
     2281 tgcgcatagc gagcaaatcc cgattattct tggctccgca acgcccgcgc tggaaacgtt
     2341 gtgcaacgtc cagcagaaaa aataccgcct gctgcgcctg acgcgccggg cggggaatgc
     2401 gcgtccggca attcaacacg tgctggattt aaaaggtcag aaggtgcagg caggtctggc
     2461 tccggcgtta atcacccgta tgcgccagca tttacaggcc aacaatcagg ttatcctctt
     2521 tcttaaccgc cgtggctttg cgcctgcgct actgtgccac gactgcggct ggattgccga
     2581 atgcccacgt tgcgatcact actacacgct gcatcaggcg caacagcatt tgcgctgtca
     2641 ccactgtgac agtcagcgtc cggtgccgcg ccagtgccct tcctgcggtt ccacgcacct
     2701 ggtacccgtg gggctgggca ccgaacagct tgaacagacg cttgcgccgc tgttccctga
     2761 tgtgcccatt tctcgtatcg accgcgatac caccagccgc aaaggggcgc tggaacagca
     2821 actggcggaa gtacatcgcg gcggtgcgag gattttgatc ggcacacaaa tgctggcgaa
     2881 aggtcaccac tttccggatg tgacgctggt ggcgttactg gatgtggacg gcgcgctgtt
     2941 ttctgccgat tttcgctcgg cagagcgttt cgctcagctt tacacccagg tcgccggtcg
     3001 tgccgggcgt gcgggtaaac agggcgaagt ggtgctgcaa acgcaccatc cggaacatcc
     3061 tctgttgcaa acgttgctct ataaaggcta cgacgccttt gccgaacagg cgctggctga
     3121 gcgacgcatg atgcagctgc cgccgtggac cagccatgtg attgtgcgtg cggaagatca
     3181 taacaatcag catgcgccgc tgttcctgca acaactgcgt aatctgatcc tctccagccc
     3241 gctggcagac gacaaattgt gggttctcgg tccggttccg gcactggcac ctaaacgtgg
     3301 cggtcgctgg cgctggcaga tattgttgca gcacccttcc cgcgtgcgct tgcaacacat
     3361 cattaacggt acgctggcac tcatcaatac aataccggat tcccgtaagg tgaaatgggt
     3421 gctggatgtt gatccgattg agggttaaac cgctcacgat gcgaggcgga tcgaaaaatt
     3481 caatattcat cacacttttc atgaaaattc tgtaaccgtt ttcacgcgct atctgctaaa
     3541 aatgttgccg atgtgaagta aacatggatg tagtacgcct gacgtgccag gcgaggagtg
     3601 agtgtgaaag cgaagaagca ggaaactgcc gcgaccatga aagacgttgc cctcaaggca
     3661 aaagtctcta cagcgactgt ctcccgagca ttaatgaatc ccgataaagt ctcccaggcc
     3721 acccgtaatc gggttgaaaa agcggcccgg gaagtgggtt atttaccgca gcctatgggg
     3781 cgcaacgtca agcgtaatga atcccgcacc attctggtga ttgtcccgga tatctgcgat
     3841 cccttcttta gcgaaattat tcgcggtatc gaagttacgg cggcaaatca cggatatctg
     3901 gtgctgattg gcgactgtgc gcatcaaaat cagcaggaaa aaacctttat cgatttgatc
     3961 atcaccaagc aaattgatgg catgttgctg ctgggttcaa ggctgccgtt tgatgccagc
     4021 attgaggaac agcgtaatct gccgccgatg gtgatggcga acgaattcgc accggaactg
     4081 gagctaccta cagttcatat cgacaatctg accgccgcat ttgatgcagt aaattatcta
     4141 tatgagcaag ggcataaacg gattggctgt atagccggtc ccgaagagat gccgctgtgt
     4201 cactaccgcc tgcaaggtta cgttcaggcg ctgcgtcgct gcggcattat ggttgatccg
     4261 caatacatcg cccgtggcga cttcaccttc gaagccggaa gcaaagcgat gcagcagctg
     4321 cttgatcttc cacaaccgcc tactgctgtc ttctgccata gcgatgtgat ggcgctcggc
     4381 gcactttctc aggcaaaacg ccaggggctg aaagtcccgg aagacctttc cataatcggt
     4441 tttgataaca tcgacctgac gcaattttgt gatccgccgc tgacaaccat cgcgcagccg
     4501 cgttacgaaa tcggtcggga agctatgctg ttattgcttg atcaaatgca ggggcaacac
     4561 gttggcagtg gctctcgttt aatggactgc gaacttatca tccggggatc aacacgcgcg
     4621 ttaccttaaa gtaaaccttt aagactgccg tgactggtca aaggcccgcc gcttaagtaa
     4681 catggcgggc tgacgaacga ataaatacag cgaaacgata gtggcacaac gagattatgt
     4741 acgccgcagc caaccggcac cttcgcggcg aaaaaagagc acctcacgga aaaagcaacg
     4801 aaatctgcct gcggtttctc ccgctatggt cgctattgct gccgccgtgc ttgtgacctt
     4861 tatcggtggt ctgtacttca ttacgcatca caagaaagaa gagtccgaga cgctgcaaag
     4921 ccagaaagtg accggtaacg gactaccacc aaaaccagaa gaacgctggc gctacattaa
     4981 agagctggaa agtcgccagc cgggtgtgcg tgcgcccaca gaaccttctg ccggtggtga
     5041 agtgaaaacg ccggagcaac tgacaccaga acaacgtcag cttcttgaac aaatgcaggc
     5101 tgatatgcgc cagcagccaa cgcagctggt tgaagttccg tggaatgaac agacgccgga
     5161 acagcgtcag caaacgctac aacgccagcg tcaggcgcag caattagcgg agcagcaacg
     5221 cctggctcag cagtcgcgta cgactgaaca aagctggcag cagcagacgc gtacgtcgca
     5281 agccgcgccg gtacaggcgc aaccgcgcca gtctaaaccg gcttctactc agcagccgta
     5341 ccaggatctg ctgcaaaccc ctgcgcacac gaccgcgcag tcgaagccgc agcaagctgc
     5401 gccggttgct cgtgccgctg acgcgccaaa accgacggcg gagaaaaaag acgaacgccg
     5461 ctggatggtg cagtgcggtt cgttcagagg cgcggaacag gcagagacgg tacgtgctca
     5521 gctggcgttc gaaggatttg actcgaaaat caccaccaac aatggctgga atcgtgtggt
     5581 cattggcccg gtgaaaggca aagagaacgc agacagcacc ctcaatcggt tgaagatggc
     5641 gggtcataca aactgcattc ggctcgccgc cgggggttga aaccctcaaa atccccccca
     5701 tctataattg cattatgccc cgtacttttg tacggggttt gtactctgta ttcgtaacca
     5761 aggggtcagc tcgtgacaac tatagtaagc gtacgccgta acggccatgt ggtcatcgct
     5821 ggtgatggtc aggccacgtt gggcaatacc gtaatgaaag gcaacgtgaa aaaggtccgc
     5881 cgtctgtaca acgacaaagt catcgcgggc tttgcgggcg gtactgcgga tgcttttacg
     5941 ctgttcgaac tgtttgaacg taaactggaa atgcatcagg gccatctggt caaagccgcc
     6001 gttgagctgg caaaagactg gcgtaccgat cgcatgctgc gcaaacttga agcactgctg
     6061 gcagtcgcgg atgaaactgc atcgcttatc atcaccggta acggtgacgt ggtgcagcca
     6121 gaaaacgatc ttattgctat cggctccggc ggcccttacg cccaggctgc ggcgcgcgcg
     6181 ctgttagaaa acactgaact tagcgcccgt gaaattgctg aaaaggcgtt ggatattgca
     6241 ggcgacattt gcatctatac caaccatttc cacaccatcg aagaattaag ctacaaagcg
     6301 taaggatctc ccatgtctga aatgacccca cgcgaaatcg tcagcgaact ggataagcac
     6361 atcatcggcc aggacaacgc caagcgttct gtggcgattg ctctgcgtaa ccgctggcgt
     6421 cgcatgcagc tcaacgaaga gctgcgccat gaagtgaccc cgaaaaatat cctgatgatc
     6481 ggcccgaccg gtgtcggtaa aactgaaatc gcccgtcgtc tggctaagct ggcgaatgcg
     6541 ccgttcatca aagttgaagc gaccaaattc accgaagtgg gctatgtcgg taaggaagtg
     6601 gattctatta ttcgcgatct gaccgatgcc gccgtgaaaa tggtacgcgt ccaggctatc
     6661 gagaaaaacc gttatcgcgc tgaagaactg gcagaagaac gtattctcga cgtgctgatc
     6721 ccacctgcta aaaacaactg gggacagacc gaacagcagc aggaaccgtc cgctgctcgt
     6781 caggcattcc gcaaaaaact gcgtgaaggc cagcttgatg acaaagaaat cgagatcgat
     6841 cttgccgcag caccgatggg cgttgaaatt atggctcctc caggcatgga agagatgacc
     6901 agccagctgc agtccatgtt ccagaacctg ggcggccaga agcaaaaagc gcgtaagctg
     6961 aaaatcaaag acgccatgaa gctgctgatt gaagaagaag cggcgaaact ggtgaacccg
     7021 gaagagctga agcaagacgc tatcgacgct gttgagcagc acgggatcgt gtttatcgac
     7081 gaaatcgaca aaatctgtaa gcgcggcgag tcttccggtc cggatgtttc tcgtgaaggc
     7141 gttcagcgtg acctgctgcc gctggtagaa ggttgcaccg tttccaccaa acacgggatg
     7201 gtcaaaactg accacattct gtttatcgct tctggcgcgt tccagattgc gaaaccgtct
     7261 gacctgatcc cggaactgca aggtcggctg ccaatccgcg ttgaactgca ggcactgacc
     7321 accagcgact tcgagcgtat tctgaccgag ccgaatgcct ctatcaccgt gcagtacaaa
     7381 gcactgatgg cgactgaagg cgtaaatatc gagtttaccg actccggtat taagcgcatc
     7441 gcggaagcgg catggcaggt gaacgaatct accgaaaaca tcggtgctcg tcgtttacac
     7501 actgttctgg agcgtttaat ggaagagatt tcctacgacg ccagcgattt aagcggtcaa
     7561 aatatcacta ttgacgcaga ttatgtgagc aaacatctgg atgcgttggt ggcagatgaa
     7621 gatctgagcc gttttatcct ataatcgcgt tcaatcattt tcatcattgt ttgatggggc
     7681 tgaaaggccc catttttatt ggcgcgtatt atgactgaac aacaaattag ccgaactcag
     7741 gcgtggctgg aaagtttacg acctaaaacc ctccccctcg cctttgctgc aattatcgtc
     7801 gggacggcgc tggcatggtg gcaaggtcac ttcgatccgc tggtcgccct gctggtacta
     7861 attaccgccg ggctattaca gatcctttct aacctcgcca atgattacgg cgatgcggta
     7921 aaaggcagcg ataaacctga ccgcattggg ccgctacgcg gcatgcaaaa aggggtcatt
     7981 acccagcaag agatgaaacg ggcgctcatt attaccgtcg tgctcatctg tctctccggg
     8041 ctggcactgg ttgcagtggc atgccatacg ctggccgatt ttgtcggttt cctgattctt
     8101 ggcgggttgt cgatcattgc cgctatcacc tacaccgtgg gcaatcgtcc ttatggttat
     8161 atcggtctgg gtgatatttc cgtactggtt ttctttggct ggttgagcgt catggggagc
     8221 tggtatttac aggctcatac attgattccg gcactgatcc ttccggcgac cgcctgtggc
     8281 ctgctggcaa cggcagtact gaacattaat aacctgcgtg atatcaatag cgaccgcgaa
     8341 aatggcaaaa acacgctggt ggtgcgctta ggtgaagtga acgcgcgtcg ttatcatgcc
     8401 tgcctgctga tgggctctct ggtatgtctg gcgctgttta atctcttttc gctgcatagc
     8461 ctgtggggct ggctgttcct gctggcggca ccattactgg tgaagcaagc ccgttatgtg
     8521 atgcgggaaa tggacccggt ggcgatgcga ccaatgctgg aacgtactgt caagggagcg
     8581 ttactgacta acctgctgtt tgttttaggg atattcctaa gccagtgggc agcataactg
     8641 acaaatatca attaacaatt gatgattttg ccaacagcct acatagcgcg atatactgaa
     8701 aattctcgca gcaactgaat gttaagccta tgaaatacga tacttccgag ctttgtgaca
     8761 tctatcaaga agatgttaac gtcgtggaac cgctgttctc caactttggc ggacgggcgt
     8821 cgtttggcgg acaaataatc acggtaaaat gtttcgagga caacgggttg ctgtacgatc
     8881 tgctcgaaca gaatggccgt ggtcgtgttc ttgtcgtcga tggcggtggt tctgttcgtc
     8941 gcgcactggt cgatgctgaa ctggcgcgtc tggcagtaca aaatgaatgg gaaggtctgg
     9001 ttatttacgg cgcggtgcgt caggtagatg acctagaaga gttggatatc ggcatccagg
     9061 cgatggcggc aattccggtt ggtgccgctg gcgaaggcat tggcgaaagc gatgtccgcg
     9121 tcaattttgg cggtgtaacc ttcttctccg gcgaccatct ttatgccgac aataccggga
     9181 ttattctttc agaagatccg ctggatattg aatgataaga aaggcaccgc aaggtgcctt
     9241 ttttctgcgt tacctgttgg cctacacagt aaagaaatta cgcgaaagat gaagcgtaat
     9301 cagacctctt ccatgcgacc cagcagggcc tgcagacgtt cctgccagcc gttctgctgt
     9361 tctttcagat ggttgttctc acgctccagc tcttcgcgct gatgctgggc attttgaact
     9421 tcctgcgaca gtgagttgtt tttttctttc agctcttcga tttccatctg caacagagtg
     9481 atggtatcaa tcgcctgctg tacttttgct tccagtttct caaacacttc taatgacatt
     9541 gtcatacctc tcctgaattg caaggcgttg atggataaaa aatcctcgtc ccgattaccg
     9601 gtgacgcctt aataaatacg agcgcacttt agttagctcc gattgtatga agccgcgcca
     9661 tcgctgtcca gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg
     9721 gtgacataat ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa
     9781 acatgcatca tgtacaatca gatggaataa attgcgcgat aacgctcatt ttatgacgaa
     9841 gcacacacat tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca
     9901 tgcctacaag catcgtggag gtccgtgact ttcacgcata caacaaacat taactcttca
     9961 ggatccgatt atgagtcaaa catcaacctt gaaaggccag tgcattgctg aattcctcgg
    10021 taccgggttg ttgatttttt tcggtgtggg ttgcgttgca gcactaaaag tcgctggtgc
    10081 gtcttttggt cagtgggaaa tcagtgtcat ttggggactg ggggtggcaa tggccatcta
    10141 cctgaccgca ggggtttccg gcgcgcatct taatcccgct gttaccattg cattgtggct
    10201 gtttgcctgt ttcgacaagc gcaaagttat tccttttatc gtttcacaag ttgccggcgc
    10261 tttctgtgct gcggctttag tttacgggct ttactacaat ttatttttcg acttcgagca
    10321 gactcatcac attgttcgcg gcagtgttga aagtgttgat ctggcaggca ctttctctac
    10381 ttaccctaat cctcatatca attttgtgca ggctttcgca gttgagatgg tgattaccgc
    10441 tattctgatg gggctgatcc tggcgttaac ggacgatggc aacggtgtac cacgcggccc
    10501 tttggctccc ttgctgattg gtctactgat tgcggtcatt ggcgcatcta tgggcccatt
    10561 gacgggtttt gccatgaacc cagcgcgtga cttcggtccg aaagtctttg cctggctggc
    10621 gggctggggc aatgtcgcct ttaccggcgg cagagacatt ccttacttcc tggtgccgct
    10681 ttttggccct atcgttggcg cgattgtagg tgcatttgcc taccgcaaac tgattggtcg
    10741 ccatttgcct tgcgatatct gtgttgtgga agaaaaggag accacaactc cttcagaaca
    10801 aaaagcttcg ctgtaatatg actacgggac aattaaacat gactgaaaaa aaatatatcg
    10861 ttgcgctcga ccagggcacc accagctccc gcgcggtcgt aatggatcac gatgccaata
    10921 tcattagcgt gtcgcagcgc gaatttgagc aaatctaccc aaaaccaggc tgggtagaac
    10981 acgacccaat ggaaatctgg gccacccaaa gctccacgct ggtagaagtg ctggcgaaag
    11041 ccgatatcag ttccgatcaa attgcagcta tcggtattac gaaccagcgt gaaaccacta
    11101 ttgtctggga aaaagaaacc ggcaagccta tctataacgc cattgtctgg cagtgccgtc
    11161 gtaccgcaga aatctgcgag catttaaaac gtgacggttt agaagattat atccgcagca
    11221 ataccggtct ggtgattgac ccgtactttt ctggcaccaa agtgaagtgg atcctcgacc
    11281 atgtggaagg ctctcgcgag cgtgcgcgtc gtggtgaatt gctgtttggt acggttgata
    11341 cgtggcttat ctggaaaatg actcagggcc gtgtccatgt gaccgattac accaacgcct
    11401 ctcgtaccat gttgttcaac atccataccc tggactggga cgacaaaatg ctggaagtgc
    11461 tggatattcc gcgcgagatg ctgccagaag tgcgtcgttc ttccgaagtg tacggtcaga
    11521 ctaacattgg cggcaaaggc ggcacgcgta ttccaatctc cgggatcgcc ggtgaccagc
    11581 aggccgcgct gtttggtcag ttgtgcgtga aagaagggat ggcgaagaac acctatggca
    11641 ctggctgctt tatgctgatg aacaccggcg agaaagcggt gaaatcagaa aacggcctgc
    11701 tgaccaccat cgcctgcggc ccgactggcg aagtgaacta tgcgctggaa ggtgcggtgt
    11761 ttatggcagg cgcatccatt cagtggctgc gcgatgagat gaagttgatt aacgacgcct
    11821 acgattccga atatttcgcc accaaagtgc aaaacaccaa tggtgtgtat gtggttccgg
    11881 cctttaccgg gctgggtgcg ccgtactggg acccgtatgc gcgcggggcg attttcggtc
    11941 tgactcgtgg ggtgaacgct aaccacatta tacgcgcgac gctggagtct attgcttatc
    12001 agacgcgtga cgtgctggaa gcgatgcagg ccgactctgg tatccgtctg cacgccctgc
    12061 gcgtggatgg tggcgcagta gcaaacaatt tcctgatgca gttccagtcc gatattctcg
    12121 gcacccgcgt tgagcgcccg gaagttcgcg aagtgactgc attgggcgcg gcctatcttg
    12181 ctggtctggc ggtcggtttc tggcagaacc tcgacgagct gcaagagaaa gcggtgattg
    12241 agcgcgagtt ccgtccaggc atcgaaacca ctgagcgtaa ttaccgttac gcaggctgga
    12301 aaaaagcggt taaacgcgcg atggcgtggg aagaacacga cgtaatgtaa atgccggatg
    12361 aagcgtttat gccgcatccg gtagtcccga aacgtgcggg ggcaaccccg cacacatcaa
    12421 taatccctcc cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta
    12481 tgagacgaga acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct
    12541 acaaatggtt aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc
    12601 gtattatgct caaccaggtc aatattgacg gcaccatcgt cattggtgaa ggtgaaatcg
    12661 acgaagcacc gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag
    12721 atattgctgt tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc
    12781 tggcggtgct ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg
    12841 agaagctgat cgtcggaccg ggagccaaag gcaccattga tttgaacctg ccgctggcgg
    12901 ataacctgcg caatgttgcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacaa
    12961 ttctggctaa accacgccac gatgccgtta tcgctgaaat gcaacaactc ggcgtacgcg
    13021 tatttgctat tccggatggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg
    13081 aagttgacgt gctgtacggt attggtggcg cgccggaagg cgtagtttct gcggcggtga
    13141 tccgcgcatt agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg
    13201 acaacgaaga gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca
    13261 tcgaagccgg taaagtattg cgcctgggcg atatggcgcg cagcgataac gtcatcttct
    13321 ctgccaccgg tattaccaaa ggcgatctgc tggaaggcat tagccgcaaa ggcaatatcg
    13381 cgactaccga aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcta
    13441 tccactatct ggatcgcaag gacccggaaa tgcaggtgca catcctctga ttgatttgat
    13501 cgattgagcc ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg
    13561 caagtcaatc aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag
    13621 tgcagaactg gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta
    13681 ccgccgggca attcaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct
    13741 actcctatgt taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg
    13801 atggcaaatt aagcccacga ctggcggcac tgaaaccagg cgatgaagtg caggtggtta
    13861 gcgaagcagc tggcttcttt gttctggagg aagtaccaga ttgcgaaacg ctatggatgc
    13921 tggcaaccgg tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag
    13981 atcgcttcaa aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt
    14041 tgccactgat gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg
    14101 tcagtcggga aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg
    14161 gggaactgga aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt
    14221 gcggcaatcc acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga
    14281 cgaaacattt acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta
    14341 cttatcgata aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata
    14401 tgccggcatc aaacggttga taaatgagtg gtgatgacca cagacaagga ggtcgcactg
    14461 ctctttttgc ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt
    14521 tgtcttcggc cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt
    14581 caacaattga agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa
    14641 gccatcatca atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc
    14701 tttattcacc agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata
    14761 agccatgttt aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca
    14821 aaaataatca tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc
    14881 tccagatcct taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg
    14941 gcaagctctt tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc
    15001 gaaaacttac tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct
    15061 ttagtactgc atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg
    15121 tcgattacga cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat
    15181 ggattaccat tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat
    15241 tgtcatttgc atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa
    15301 aggttaatta cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata
    15361 tttattttcg ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat
    15421 caacgtcggg acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgcccagtt
    15481 acccgccagc agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg
    15541 atcatgcaag cgcttaacag ttactgccgc tgttggccaa agcaagcaca caaggcaaaa
    15601 cgccgcggtc tgaatatcga gtaaattttt acccgccagt gagaaaagca ccagcatgcc
    15661 tgcgaaccac aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga
    15721 gaataaccat tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta
    15781 cccttttgac aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact
    15841 gtcaatgaag ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac
    15901 aacggcgcat gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc
    15961 ccctactttc gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcctag
    16021 cctgccactg aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg
    16081 tgctgcaagt aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt
    16141 aaaaatcaaa agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc
    16201 gaaagcgaaa gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac
    16261 caaaacacaa agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta
    16321 ttacaccgag acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct
    16381 gaccttcgct gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa
    16441 atgacaaaaa gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc
    16501 cctgcggggc ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat
    16561 cctttagtga tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt
    16621 tctaacctgc gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg
    16681 gaaatgtaca tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg
    16741 caaaacgtgg acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg
    16801 aaagacatcg gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa
    16861 gagtctgacg aactgatcgc gaaaaaattc gcggtgctga aagagcaggg cctgactccg
    16921 gttctgtgca tcggtgaaac cgaagctgaa aacgaagcgg gcaaaactga agaagtttgc
    16981 gcacgtcaga tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt
    17041 atcgcttacg aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca
    17101 caggctgttc acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa
    17161 caagtgatca ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct
    17221 cagccagata tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca
    17281 gtgatcgtta aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc
    17341 atatccggca cttactttcc ttaactcttc gccttaacgc aaaatctcac actgatgatc
    17401 ctgaatttcc tcggctgaag cacggttaag cgtcagtaga tttcgttgtg tcgccagcaa
    17461 tacaaatgag ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg
    17521 cgcctcaggt acttcttctg ccagcatcat aaatgggctg cgttgtacca gttcgctttc
    17581 cgttacccga cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg
    17641 gctgctgatg ttcgccagat tgttatccag ttgttcgcgc acatcaggac gaatacaaga
    17701 gatatgaata tgaaaatggt tttgcgtacg cccggtgcgg gagttgatcg ccaaagaaac
    17761 cgcgcgatcg ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc
    17821 caaccaaaag aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat
    17881 acgatacgtt ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta
    17941 tccggcattg ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg
    18001 caaacattcc tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca
    18061 gtaaccaata ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc
    18121 ttttttcatc tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac
    18181 aaataaaaaa cccggtcgcg agaaaccacc gggtttgata attatcctgg ggaaatcagc
    18241 gtttgctgat ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc
    18301 agccgccgaa ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact
    18361 ttttcgctac ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt
    18421 ctggcgagta gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga
    18481 ccactttatc gaccaccgac acggttggct ctgcgaggat agactcactc ggcgtgacga
    18541 tttcgaattt atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga
    18601 tcagtacatc gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat
    18661 ccagaacttc gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt
    18721 tgttgttgtg atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag
    18781 agcttttcgg attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat
    18841 ggatctgctt cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg
    18901 agttatccgg cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt
    18961 ccacgtcata ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg
    19021 tcgcttgttt acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct
    19081 gtttccagtg ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat
    19141 atgaaacgtt aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg
    19201 ttaaccctac gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa
    19261 agcgtgccag aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg
    19321 gtatatgttg agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg
    19381 ctttgtccat gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc
    19441 aataaattgc agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct
    19501 gtcatcggtt tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc
    19561 gcgcagtcca gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg
    19621 tcgtggtgaa ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc
    19681 agcagatcga tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga
    19741 gaaccaccgc gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc
    19801 tcgatgaaat gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg
    19861 tgttttttac ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat
    19921 tcaacttccg gaaccacaac gaattcacag ccaccggcaa tggccgcagc caacgtcaga
    19981 tcgccacaat aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg
    20041 tcacgcagac ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg
    20101 tagtcagtgc ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc
    20161 atttcggtca gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg
    20221 tcgataccac gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat
    20281 tccgggaagc gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agaaacgctg
    20341 taacggtcta gctgtaccat acggtcttca tacagaccca gatagccgtc ataaatgccc
    20401 attacttcca gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg
    20461 cctggcgcat caccgccgct tgtcaacaca ccgattttct taatcatgac tacctctgaa
    20521 ctttggaatg caaaataaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa
    20581 cgaatgtgca aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg
    20641 gcggtatttt atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata
    20701 gaccgtttac cctccctggg tacgacggaa caggggtcct gatggataat tacatccgat
    20761 cccggaaaac gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc
    20821 aaaggcagag agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc
    20881 cgcgtgcgaa gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt
    20941 tcttgccgtt cctcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc
    21001 atgcgtaacg cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg
    21061 cgatgccagc cgtaccagga caaccccagc gccagcagaa ttgcgccgtt catcataaca
    21121 tcagactggt aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc
    21181 caccgctgaa acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc
    21241 ccgacgcctg gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac
    21301 aggaatagtg ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct
    21361 ttaccgtgac caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt
    21421 aaattcgtca acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc
    21481 accgacccgg tataccacca tgcaaaaatt ttaatcagca atagcagcga agccatcgcc
    21541 gtcgcagcaa tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc
    21601 gctatcaact gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc
    21661 aaattgagga taaaaaaacc cccacatcat gtgggggaag acagggatgg tgtcacaaaa
    21721 aagtacctaa cctgatgata taaatggatt tatgtcaacc gctgtccaca tagcgtccac
    21781 atcgaccata aataagcccc tcaactgagg ggctattttt ttgatcacat ccacataatt
    21841 tgctgccctg acggcaacgg gtgcggcctt acggcgtgga cttctcccgg cttcacgatg
    21901 tatcgctgta ccgactcata agtgatgaac gtggcgctgc aattcacgtt ctggcactgg
    21961 tgataacgct cttttgtcgt gtcagtgata tagcgacttg tacgcgcatg tgcggcatgc
    22021 tggcataaag gacaatgaaa catcgcgagc acctcttccg gttttgttga tggtgccatt
    22081 ttagttaatt tatccttata aaacaaacag ataaataaac cacgtcactc atcatcttct
    22141 gtttcgtact ccacatcaga aagccttacc tcaagctcca aggacgtcgt gaagccgcta
    22201 ttattgagac aatgtgtcac cttagtgatg gtccagtcct gctcgtctat gacgcgctta
    22261 aagcctgaca ctttaaccgg tgtttccgtg taaatatctg cacgaccggt agccaggctg
    22321 atggagaact ccgctacacc ccgttgcagt ttatcccact tcgcctgagc ggcgcgcatg
    22381 gcctgcgctt ttgtggcata taccgtggtc agggcaaaaa cgttgtcagc ttcaccggcc
    22441 atgtattcac cttcgcgcgc ttccggtact tttggcgctt tcttctgcgt gaccggtttc
    22501 gctttcgggt gctccagtgc gcgcaggtgt ttttctttct ttttgcgttt cagttttacc
    22561 ttctgctttt gcggcttcgg gtctttggtg tgtaaccact ttgccgttac gccggtgtaa
    22621 gctccacggt cagcaatcgc aaaatgatga cggtcgccgt cgctgcgggt gatggtaatc
    22681 tgcgggattt ttttaccgct ggccgtcacc ccctgccccg ctttgagaaa cagcagtttt
    22741 cccattttta ccgacacctc gccgccgttg cgttcagcaa gacgggtcag gaatttcgca
    22801 tcagactcct gcgactggtc gatgtgtgga atttttattc cggccagtga cggagcgaca
    22861 ctggcttcca gcctgttacg ggaggctatc gcctcaacaa tcgcaccgag cgtaatgtca
    22921 tgccatgagc cttcacggcg ggaattgagc gtcccgcgaa aatctgcact ccgggcgcgg
    22981 atggtgacca catccggcgc gccccggtgt tcaacctcat caacggtaaa tttccctttg
    23041 cataccaggg caaaaccttt ccagccgata tacaccgtca ggacagcgcc acgaaccggc
    23101 agcccgacct gcccgtcggc atcgttcagt tcaatatcaa gctggtcagc ctcaaagccc
    23161 cggttatccg tcagggtcat gctcatcaga cggtcgctga tattgccggt aatatccctg
    23221 ctgtcgagca tcagcatgta atccggcgtc agcgtactgc ctgcatcaaa tgtcagcgca
    23281 tccagcatta tcccgccccc gtcatacccg tgaatttagt cgccatactg ccagccttac
    23341 cgatgagcga ttccgcctgt ttaccgatat cgccataaag cgcggccagt gactcatcaa
    23401 cgcgggtgag cgacagcgta aaatcaattt tccgtggtgt gccgtctgca aagaaaatac
    23461 tccctgtttc actcaccttg ctgatgacat acatgccgta aatcatgccg gtgccatcca
    23521 gcaacggcca cgcccggcct tcctctgcca tcagtctgag cgtggtcatc gtcagctttc
    23581 cgccagtcag ttcgggataa agcacaccgg caagtgtgat gttctcctca cctacaccga
    23641 gaaactgata ggcatcccgt ttaccgatac gggaatttga cggccagcga taatctgatt
    23701 cacgctgcat ggtctggtgt ggcagcgtct ggcgcataaa aacaaacata cctaacgcga
    23761 gcatcatttt tcgtcacctc cttaaccgtc atgcatcatg ctggcacggg cgcgcgcacg
    23821 tttatcccgc tcgtattttt cgagcgcatc ctgtaactgg cggtcaagct gtgtccccgg
    23881 tgcagtacca cccgtcaggc tgatgtgata ttcgttttta ctctggtcca cataagagcg
    23941 gccagccggt gccgtgaccg gctgataagc ctgatagcct gcataagagc tggtcgccgg
    24001 aatataacca ccggtgccat acgtggcggc atgagttctg gcggcggtct ggtcaagtgt
    24061 gtctgactct ttgttgatga ccccgagctt ttccagtacc cagtcaatgc cgctgcgcaa
    24121 tttgttgaac gcattaagcg gcagcatcag cgcgtcagcc agtgcctgcc cgaacatgac
    24181 gcccgtgtca cggcaacggt tcagggtgtc ctgggtggct ttgaccgggg caatcaggtt
    24241 tttaaaccac tgccacgcag cctgtaactt ttcgcccagc cagtcaaaca ccggtttcag
    24301 tggcgtgaac agctccccca ccggcgcaaa tgccgctttc agcccttcaa ccacaccgcc
    24361 aaagaatgcg ctgacaggct cccagtattt acggataagc aacgccccgg cgacaatggc
    24421 ggccaccaca gccacaaccg gccagctaat cgccccgatg gccgtcataa cggcactacc
    24481 aaccgtcgtg aagattgccc ccattgcgcc tgctgccgcg atgatggcat tgatgccggt
    24541 gataaccggc caggctacaa gaccaattgc accgatgatg ccagtcagcg ccagcgcgcc
    24601 accggcaatg aggccaatgg ttgacgccag tgatttgttt ttctgtaccc agccgtcgag
    24661 ttttaacaca tactttgtgg ccgtctgcgt gagcttacgt agcgcgcctt cctgctggtc
    24721 aaacaggtct gtccccaccg cctcataagc ggactgaaac tccttaaagt caccgccgag
    24781 gttgtcctgc atgatattta ccagctctgc ggtcttcccg tctgaggctt taaacgcagc
    24841 ggtcagtttg tccagctttc cggttgacgc ggcagtcatc agcacagcgg cggctgagct
    24901 ggcctcctcc ccgaaaatgg ttttcatgta ttcggcctgc tgggcagtac cgagccggtt
    24961 tttctcaaag ctggcctgca tttctttcag aatggtaaat actggtcggg tgtttccctt
    25021 actgtctgag gttttcacgc caagctcttt gagtgcatcc catgcttttc cagtcggtgc
    25081 ctgcaggcga cttaacacgg cacggcttcc cgtccccgcc attgagcctg tgatttttgc
    25141 atcatgcagc gccccgacca ttgcggcggt ttcttcaatg ctgacaccgg cattttttgc
    25201 cactggtgcg gcataggtca gcgcatcgct catgccgtca aaatcagcgg cggttttgtt
    25261 catcgtcatg gagagaacat ccccgatatg agcgacctta tcgtttgaaa gctgaaaggc
    25321 ggatttcatc cccatcagca gggcggcgtt ttcttccatt gtgcggcggt tcgccagcgc
    25381 catattcagc gtgaccggcg ttgccgcctg aatggcatca acatccccac ccgctttcgc
    25441 aatgataatc tgtgcaccgg ccgcatcatc cgctgaggcg gcggtgttgt caccgagctg
    25501 gcgcgcctgc ttgcggagtg cagccatttc ggcggagtct tttgccacac cgagcacagc
    25561 ctgcaattct gagtttttct gcgcaaactc ataacctggc atcagcagct taactccggc
    25621 tatcgttccc gcagcagcaa tccccacacc ggcagcgccc actgaggcca tatttccggc
    25681 cagttccttt ccggcctgat aacgctgttt gactgcgtta agttttgcct gttgcgcact
    25741 gacacgcgcc agcgcgtcac gctgccggtt aagctgggcg gtggtttcac tgatacggtt
    25801 tttcagtccc tgctcatcat gtgcaagatt gcgggtatta attcccacag cggccagttc
    25861 ccgctgctgg cgtttaacgg aatctgtcag gcggttatat ttcgcctgta agtcctccgc
    25921 cgcacgcttt gcggattcca gcactttcgc ctgagcacgg gtcgggcgtt cggtgttttt
    25981 aaactgtgtg gcaagggctt cggcctcctg ccgtgccttt tcaagtgcat gaccagtcac
    26041 ggcgagctgt gcactggtct tgcggaatcc ctcaatacgg gatgcgtgac cgttcagctc
    26101 gcgcagtgat ttttgtgttt cccggatatc ccccgacagc aacttactcg ctctgcggat
    26161 ggatttaaac gggcgggacg cctggtcaac agccctgagc aatacctgta attttacatt
    26221 gttactcatt cgtgtttccg cttcgcctga gcgccttttc gcgccatgtg atgagttcgg
    26281 tcaggctcat gggatacagt tctgatggcg gccagtgaaa tatcactgcc acatccgcca
    26341 tcaggtcatc gaccgacaga tttttcggaa gcgtcactgc accgagttcg gcgacaaaaa
    26401 accgaccacc ttaccggcca gcgccacaag gtcaggcagt tccagcgcgg cgacttcctg
    26461 ctcggtcagc atcggtgccg tcatgcgcgg cagcacctta atcagtgcat cgacttctga
    26521 gtttgcgacc gcagccagac tgacaccgcg cagcgtcccc gcactgggtt tcatcagcgt
    26581 gacctgttcg ataacctgct caccacgctt gaccggattg tccagggtaa tcacattttc
    26641 tttgttcatg gttttctcac ttctgaatcg gggttaaccg gtcagccagg ctgaccggat
    26701 gaaaattaca ggccgatatt gcggcggtgt tgctccagcc ggtcgacgcc gttcaccttc
    26761 tcaatcatgt tgatggtgtc gatttcgacc agctccttac cgtccatcgt cagccggaaa
    26821 taggtgcaga ccacggagat tttcgactcg gtgtcttctc cctgtttacc ctcgccggtg
    26881 tcgatttctt tctgacggcc acgcatgacc acctctacgg ccaccgtttc gccggtatcg
    26941 tcacgctggt aagagcctgc aaaacgaatc ggcacggcat ccacaccggt tgcggcgtaa
    27001 agctcccaga taaccgaatc cgggaagccc ccgagcgacc actccattga cagcgcatcg
    27061 tcatcaaggc cgaggtctac cggtgcgctt ccgttcatcc ccgcaccgcg atagttttcg
    27121 agtttacggg tcagttttgg cagcgtgacg gactttgcaa cgccctgata gctgtagccg
    27181 ttcagaaaga cgttcattaa cttgagtttg cgcggcattg ccatcggtca ggctccttaa
    27241 ttgctgttaa ccgaggtgac cagatttgcc aggtatttat cggtaatacg ctggcgcagg
    27301 gtcaggtttt caagaggagg caccggggta tagtcgtagt cgatatacag ttttccggcc
    27361 ttgagggttt ccgcatcgtt ggattcttcg ctgaaccagc aggtcgcatc cacgatatag
    27421 ccgtttgttt tcagctcacg gaatttggca ttgatgccgt caacgatgtc gcgaatcagc
    27481 gttgcggtga tgggcttgtc caccgcccac atgtgcgcct cagccatcgt gtcggccagc
    27541 acctgcgcgg tgcgggtgta gttttcaaag aggaacagcg ggtcatcaga gcaggtacgg
    27601 ttaccccaga agcggaaacc gtcacggcga atcagcgttg tgacgcctga ctcgttaagc
    27661 aggtcagcat cggtgccgga ctcctgcaaa tcccagaata cggacgcgct gatgccggta
    27721 acaccgttca ccccgacgtt ggacagcgtt ttatgccagc cctgctcctg gtcgatttta
    27781 gcgcgcagac ccagcgcacg ggcggtggca tacgcggtgg cggtggtact ggcgaccgta
    27841 tcccatgcga ggaaatccgg ccagatgacc atcagctcac gctggctgaa attctgacgg
    27901 taggctttca cctcggaaac ggtcttacag ccccatgcgc tgatataccc gaaagcgcgc
    27961 agcttctgac agactgatgc cagtgcaaca gccacctctt tggtgtccag tcccggcacg
    28021 ccgagaatac gcggtttaac accggttacc gactccgccg ccagcagggc tttcagtccg
    28081 gtgtactgac cgttttcgtc ggtggtgccg atgatattgg aaacggtctg cgcgagtttc
    28141 gtttcctcgt cgtcgccggt gccgtcttcc acacgcacga caacggtgac cggttttgac
    28201 tggtcggcga tggcctgcaa cgatgccgcc agcgtgcctt ttttaccggc ctttgcaatt
    28261 gcgctctgca cattggtaat cagcaccggt ttattgaggg ggaaggtttc cgcatccgca
    28321 tcgctggccg tgcagaccat gccgacaatg gcagtggata cggtggaaat gacgcgggtg
    28381 ccgtcgttaa tctccagcac ctgcacgccg tgatgatagt cactcatccg tttaactccg
    28441 tggttaatgg gtgcaactat tttctgttgg gcagtgcatg agacgctatt tgacctggct
    28501 ggtcagtgga tgaaacaaca gataaagaaa atgcgggcaa ttcgcccgca tgtcctgatt
    28561 tgtacacact cattttccga ctgacagttt acatagccca aacgctatca aatctgacag
    28621 cctgctatga gcgaaaagcg ttaaatctca cgatatctgc taccgggaaa aatttcgatt
    28681 atcttcgatg ggaactttga taatgtagta aatgccattt tcagcgatca cttttcaagc
    28741 gtgtgtaaat gattaatgtt aaaaaattga cttcactgaa ataaggttgc tagtattttt
    28801 ggagcaacca ttaatgtttt ttatttaagg aatgtaaatg acatacgcac tgattgataa
    28861 ttcaactcta actgcagtcc aaagaatcga ggggaaagta aaaacaagat cgaaggactc
    28921 agtagataca gacataatag catttgagaa ctatatacag tctattttgt tctatgatca
    28981 cgttatcgcg gttgatgatt atattccaga gtaccgtggg agtaggattt caagtttcag
    29041 agagatatta tttcttgata aagcacaata tgggttggat gaaattgaaa gtgaagcaaa
    29101 aaaaatatct gatgcattga aacctaaaat tcgtggtgga acatttgtta atgacgattt
    29161 taaaaaacta attgaacttc ttcagacaca tatcatatgt acatgggaca ttagttcaag
    29221 tgtttatcat ctaacactga agaacttatc tgatggaggt aaggagtttg aaaaatatgg
    29281 taacattgct gctgccattt tttcagaact gcgtgatgct gctgagtctg gtaatagaac
    29341 aaatggcgat gttgaacttc tcgatcgatt tggcaaccct attcgcaagg ggtacaaagt
    29401 tcccggagca aaatgggggg atggcacatc tgctggtgaa gcatcagatg caatcaaagc
    29461 attcgtggct tcgcttgtat ggctggctaa tcgggcagta ttttattctc tcacagctaa
    29521 gtaccttcaa gcagatactt ttttatatcc attacgtcag gcgtatcaac agacatattt
    29581 aagccaaaac tgtcagtatg gttttgacta tgctaaacat attgtagaaa acttttccac
    29641 ttcgttaagt catgatctca ttgatattca ttctggtggt aaagctgtag caacagcaac
    29701 ttcgcttcct ttattttcgg catggctcgc aaaagaaact ggtgatccaa gttgtattgt
    29761 tgaagctgct tataacataa gggacaacaa cgaatttgta agggcccgtg agaaaatgcg
    29821 agaaataaag cgcttgtttg atgaaaatga attgcctcaa gctaataagt cagtaagagc
    29881 attgcttact gaagtatcga aagaatcaga ttatattcga actaagtatg ggctcaagac
    29941 tagacaaggt gtacctgtaa ctaagcttgt acatgtatac aatactgttg cggctattaa
    30001 ttcccttccg aaactaccga cctttaattt taaagttaaa gttcctgatt tcttatatga
    30061 tcttaaaaaa cccaaggggt ttcgtgctct ctatcgaaat ttgactaatg atctttctac
    30121 tgtgtggtcg ttaggagaag ctcgagatat tcttggaagt aaagttgtga tagataatca
    30181 tgcaagaatt tacagtccga agcatgaaga accgcgtttc aaaaactctc attccccata
    30241 taagagcccg atgtagctta agaaaaagac acgcccacct tgtgcgtcta cttctggcac
    30301 agagcggact ctcagattag gctttactct gtgccataga tatgtaagct cacaccagag
    30361 ctcatacaac ttattgcggc atttccggcc attcaggatt tgcaggatcc acacgactga
    30421 ccagaacact gtagcgttcc catgcctcca gtcgactgcg ctcctcatct gttgccatat
    30481 tcagcctgac agcgcgctcc agcggcaaaa tcacggattc agcatctgca agaagtctgg
    30541 ctttccgatt ttctgcctgc tgctgcaatt cctctgccgt ataaattcgt ttaatcactg
    30601 tacagtcctt aaacatccag ttccctgaaa tgtccgcccg tcggttagca gtaatatccg
    30661 gcacttcaac aacacttaat ccatccggtc tgatagctgt cacatccttt tccacatagc
    30721 ggataatatt atctttgtcg tacgctattt ttatcgtgtc atcagcaaaa tacttttgtt
    30781 cttcgtacca gttcttacca tcttctgaaa aaaaccagac aacatcaaag tcctttgtca
    30841 attgatattg ttcaaccgtt tttggattac ctgccgttat atttatcaaa tgctgcataa
    30901 attatacctg tgccacgtta taccaagtcc cgttaatgta tttctgcacc ggtctgtaat
    30961 atacgccacc aatgttatcg gcagagtttg agccggtatc ctgaacaata atgccggaat
    31021 atacacaccc ggacggtgcc tgatgtgtcc atgtcatacc attgttcgca ggtttgtatg
    31081 tggcagcacc accaagccgg atatcccgga catagcgtga atcaaagttg ccatagttag
    31141 atggtgatac ctgcccgtta acagcaaaag tgatgctgtt atctgtattt ctctgactgt
    31201 aaaaatgcca gcctgcatca tcacctaatt cagccaccac aggacgactt gagtttcccc
    31261 acaaattgaa tgcggcttcc ttcgtggatg tattgctgct gctgaccgtg aactttttcc
    31321 cgctaccggc acgtactttg gtacttgaga caatatcacc tgtaacactc aggccatgcc
    31381 ccattgacac tccgccattg gcgttattga tagtcagcgg tcttaagccg ttccatgtcc
    31441 caaatttatc gccagaggcc gtcagcatta aatatgtgct gccgccatca ttcctgataa
    31501 agaatccata attgccataa gcaatgcgca gaccattagc actgagtgat gtaatttcac
    31561 ctcttgaacg gagaccataa gcggagctga gtgataattc ttcctgagcg tcattgtttc
    31621 ctgtcgccca gcgaattacc ccgccctgaa ctgtttcatg ccagatagta tctccttctc
    31681 caccacgaaa ctttctgaga tattttttgc cgcctctggt gcctgaacat aaggccgtag
    31741 acatataggc attctggctt ccgccatcct gattaatcgt tccggtcatt gagtcgccct
    31801 gacgattcca gtcacgacgc cagccggggg agtagccgtc cccatgattt atgtaagtga
    31861 attgcgcgct ggttgtaccg ccaccgcttg atgttgttgg cgtggtcact cggatagtga
    31921 ttgcagattt agttcccatg acctcgacga cacaaccagc caggtggata tcaccacatc
    31981 cggtatccgt aatgattttg ttatttgcat atgaccagga gcctttgcac atccagtacg
    32041 gatgattaaa tgcaccacgg gaatccagcc attcaataaa ctgagcggtt gtccagtttc
    32101 cggcttcagt gctcaaagcg ccgctataag cacgacaggc accgatattt ttcgtgaagg
    32161 tatcctttcc cggaatatcc gcaccgttct gatctttctg aagacgtttt tcagcattgt
    32221 cataggcaga cttcactgct tttggcgttg cggccagcgt ttcagaatcg ctgttgatgg
    32281 cactacttag ctggacaaga ccttttcgcg ctgtggtggc atcctgtgca gtgtatttcc
    32341 cgttagcaag gtcatacgct gtcttaaccg cctttggtgt tgccgcaagc gtttcagaat
    32401 cgctgttggt ggcgctactg agctggacaa gaccttttcg cgctgtagtg gcgtcctgtg
    32461 cagtgtattt cccgttagca aggtcatacg ctgtcttaac cgcctttggc gttgccgcaa
    32521 gcgtttcaga cgtgctgttg gtggcactac tgagctggac aagacctttt cgagctgtgg
    32581 tagcgtcctg tgcggtatat ttcccgttag caaggtcata ggcggccttt accgctttcg
    32641 gcgttgcggc cagtgtttca gacgtgctgt tggtcgcact gcttaactga gtaaaacctt
    32701 ttgcggtcag cgaggcgtcc gggtgacgtc gtgactgttc gtgctctgca attttgtcat
    32761 caacgtaatc ctgtgtcgcc atcaccgttg tggtgtcaat ggtcagctcc accgaggcca
    32821 cactgctgac gatgatgacc atgcgacagg tctgcgaacg ccctgagcct tcggcaagag
    32881 ctggcttata actttcggcc atgttcgcca cggcaattaa cgttcccgca tcatcgtaca
    32941 ggccaagctc acgcatccag aaaccgccca cctccggcgg aataaccagc tctgcgataa
    33001 tataattact gtttcgtttg tcctggctga ttttgttcag cgtatgtcgc cagacttcgt
    33061 ggataagccc ggtctgtccg gcatccggga caggcaattt accaccgcca tccccgacgg
    33121 ccatcgtggt aatgttgacc ttccgccctc ccggtgcggt tgccgctgcc agctttgctg
    33181 caccggcagt ggtgataacg gttctgaatt ttgtgctcat tattcctcac ttatccgggg
    33241 taaaccgtaa ttacatcgcc gtcgtaagcc acaccaccgg cgaacaggta gccgggaatg
    33301 tcccgggtaa tgttcaggcc aataagatga cggcttgcag gtttggcatc agcaataagc
    33361 cgttccattt cctgatacat tgcctctgtg atgccgcttt ccagtacacc aatatcaagc
    33421 cggaaggtgc cgggcgggtc actgttttcc caccactccg tcacgttgat gagatagccg
    33481 agcggctcca ccacacgccg gattgcgccg atagtgcctt tatgacagtg gatgaaatag
    33541 gcatcgcgga taacggcgcg ttttgtcgct tccggccact tttcatccca cctgtcgacc
    33601 gaaaacgccc acgccagcca cggcagcaga tttgccggac aggtatccgg gttccacagt
    33661 tcacgaatcc tgaccggcgt tttttcaatt tccgcacagg cttttgcggc ggcaacttca
    33721 agcggtgatg agccggtcgg cagcagtcgc gaatcactca tccgagcctc cggtcacgac
    33781 gcggtattcg gtacagaaag acgcctgcgt actgttgagc acgatgtcgg ccagcggtgc
    33841 agtcagttcg acacgctgca cgccttccac atgcaaagcg gcataaatgg cagacagacg
    33901 gatgtcgcgc cccagccggt gctgtgccgt gatgtacgct tccagttttt tcacggcagc
    33961 agcgcgtatg ggttcgcttt cgggaccagg gtaaaggtaa agcgtggcgt ttatctggta
    34021 ttcaacgatg gcggcagact gcacggtcac gcggtcggcc accggcctga cgtcctcacc
    34081 attaagggcg ttacgcacca ccgccagcag gtcttcggat gcgacaccgt tattttcacg
    34141 tgacagcaca gagatggtga cgcaggccgg agacggactg gttacagaaa tatccgcgac
    34201 acgcccgtcg gcactgcgac catgatactg ataggccccc accgacccgg cgacgcttaa
    34261 accttcaaac gcctgctgaa tacgcagacg ataatccgtg tcagattcca tcactgccgg
    34321 tgtcggcggg agggtcgaat catctgccgg ggtgataatc aggcgcgtgg tgttgtaatt
    34381 ggcaccaatc acatcaaggt cattaccggc ggcacaggcc agcattaccg cccgtgcggc
    34441 ctcattcaca cgctgacgcc agataagctc acgataagca ttttcctcca gcagtttgac
    34501 gagaggctcg gattccagcg tcagggtacg ggcgaccgcc tcctgctggt cttccgggta
    34561 aagggaaatc agtgtcgcct tgcgttcggc aagaatggtt tcaaagtcca gctcctcgac
    34621 cacatccggt gcgggtagct ggttcaggtc gataatcggc atggtttcaa ctcacaggga
    34681 tggttaacga aagtggctgg ccggtgtcgt tgtgctggcc ggttaacgtg accgtcattc
    34741 gcccgtcaaa gctgcgcgcc gtggtgacgg atgacagggt gacgcgggct tcccatttca
    34801 gcacggccat gtaacaggcg accttaatct gcaactcaag cgccggggtc tgcggctggt
    34861 caatcattga cgccagcaac gagccgtaat cacgacgcat cacccgtgag ccgaccggcg
    34921 tgcgcaggat atcgccaata ctctggctga tatgctcaag gtcagagaca gtcaggccat
    34981 cactgcgatt cattccgaga taacgcgctg tcatagagga ctcccggttg tgccgccgct
    35041 gtcgccgggg tgtttatggg tatgcagtac cttcccgttt gatgagagtt caccgccggt
    35101 gtgttcaatg ttgccgcgca tcgtcccgcc cttctgcact tccagcgtgc cagtgatcag
    35161 cttgtttgtg cagaccacct ccggcgtgtc cagagtgacg cgggttgatg ctttcaccgt
    35221 gaccaccggc accgtggcag taacagaatc agaagccgtc acgctggccg ttttaattcc
    35281 gcttaccgtg agtgcactgg tttcaggttc atactcaatc accgccccgt cagggaaacg
    35341 gatatgcagg gcatccgccg acgcagacgg cgcggggtta tcaccggaat aaatccccgg
    35401 cagaacgaac gccgtgtcga gttcaccgcc tacggccaga atcagcacct gttcccccac
    35461 ggaaggtgcc caccatgtgc gcgaacgtcc ggcgcgatgg gtcagccact gaagccagtc
    35521 ggtgcacatg ccgccggtct gcacacggca gcgaccggca ttaaggtcgg tttcgacgat
    35581 aatgccggtg cggatcatgt tgcgcagtgc gcgcgcgagt tcctgaatat ttgcgagagt
    35641 gttcataacg ggaaggatgc cgccgggtca taccggcggc aatgtgacga tgaggtgtca
    35701 ggaatggcac aactaacggt cgaggtgagc caggataatc tcttcaatca tctgcacatc
    35761 ctcaccggta aagccgagca ggggacgcgc cggataatga attttcttac cgtctttccg
    35821 ggtttcttcc gacagaccga actgatgcac actggctatt ttcggtgact tcccgccgta
    35881 aaactccatt gatgcctgtt cagggctggc gcggatatgc aaaaaacgac tggtgataag
    35941 tttcgcaaac atttttcgct taacgcgacc ggtctttttt ctggcgctct gctgctggcg
    36001 tggtgcgtag ggtgtgccgt ccggggcttt ctgtgccatc acccgacgct gctgactctg
    36061 acgcagacgt ttcgccagtt cggcgctcag tcgccgacgc cctgacggtg acagcgactc
    36121 aataagtccg gtcagcctgt cttcaaaacg cttaaactca ttcatcccac ttactcacca
    36181 gttcgccatt gatataaagc tccatcgggc gggtgaccgg ctccggcggc ggaggttccg
    36241 ggatattctt cacatgcagc gcgccgtcca cctcactgac cagcgtgcgc tcggtcagca
    36301 tcaggctgat gctgatatca aagctgctgt cattgttgat gtctgcataa aacgtgaagc
    36361 cctttttctg gccttcgtcg gtggtcatga tgtcgggctg attttcccgc agccacgcca
    36421 gcaccggcac gatgagcagg tcaaaatcac cggtaaagtc ggtcacaatc acgttgagcg
    36481 tgtaacgctt ttcgaatgac agcgacgtcg ccagtgtgga ggcaatactc ccgttatcca
    36541 cgaatatccg cagcatctcg gggcttgttt tcagcaccgt gacggcatca gtcagcgccc
    36601 tgcgcaggct gtcgggtttg agcatcgttt tcgtcctgac agtgtttaat catttttacc
    36661 tggctggcac agcgtgccag cgcgttctca agctgccgga tatcagcact taaatcgccg
    36721 ttcgtctgcg ggtcactgcc cggcatcggg caaaggctca ctttcgggca ggcgttgtgg
    36781 acaatcactg gcgtctgcgc aggctgtgcg ctggtgcaac cggcgcacag catcaggcag
    36841 gtcagcaccg taccagcggc gaaaatcttc gttttcatta agtaacctcg tgatggtttt
    36901 ctcgcgctga gcttcacgct tcgcggcgtt ctccagttcc tgacgcagtg ccacctgcgc
    36961 cagctcgttt ttgtctgccc tggtgagggc aacatgaagc tggtttttca gcatggtgat
    37021 ggtcgtctgc tgttcactgg cgacgttgtt cgccctgtcc agcaaggcgc gcaggctggc
    37081 atttttgtgt ttcaccagaa acagaccggc caccgccagc gataacaaca caaccagcac
    37141 aatcatcagt tttgacatgg ttcccgcccc tcaagacgct gacggcaggc cgtacgtatc
    37201 agccggaaaa acagcgatgc cacgagataa atcagtgcgg taaaaatcca cccggcagcg
    37261 accagcgaga taaacgtcgc caccatcacc atcagagccg ctgcccgtct gcgccacggc
    37321 accggctgca aaaacagcga cgtgacaatc ttcacggcca gcgattccgg cggcagctcc
    37381 cgcccgtagc gttccagcac atactccgcg gcatacacgc cgacaccacc ggcaatcaca
    37441 cagataaccg tcgccagaat cgcccatgca gcgacaaaac tgacggccac gctctgcggg
    37501 taaatcaggg acagtgccag catcagcgcc agcgacacgt tcagcatcag tgaaagggat
    37561 aatttcttca tggtgtttac tccgtttaag ccggtacgcc gccagcggtg cgccagacgg
    37621 tgaccagttt ttccagtgaa tgctcacgct gaccgtaacc ggcacccggc agggacgccc
    37681 agatattgcg acagcgtgaa atggcgcgct caatgcgtcc cgcccggatg tcatccagtg
    37741 caccgcgttc gcggatcaac tgaatggcga gcctgtcctg tgacaacgga ctgaaatccg
    37801 gcagggcaag ctgtttgcgg tagtgcggcc agaacaggta aagctgctga tagcgaccgg
    37861 aggccgtgga tttttcaccg cgacggttaa acaccttcgc cggtcggcca tgtgcgaacg
    37921 ggtggtcact gtagtcggtg aagatttccg gcttcccgtc cagtccggtg actatcacgt
    37981 catagccccg gtttttcgtc agcggatgat tcgccgtccc ttcggacacg gccagcatgt
    38041 cgagaaaggc ggcgatattc tgatgtgtgc taattaccgg cattacggtt tccccctgcc
    38101 cttaaagcgg cgctgaatgg caatctcaat cacctgataa ccggcgatac ccagcatgga
    38161 gccgatgccg cacaccgcag gcagtgacag gtcaggaaac tgcaccagaa caacaccggc
    38221 aaccatcgag acaaaaccac cgagcaacat gcgcccgata aacagacgcg gagtgatggg
    38281 ttcaccaccg gcaagcacct tgccgacaac aatcagcacc ccaatcatga aaagcgacag
    38341 gacgcttttt tcttctgctg tcatgcgtta ctcccacaga ttgacagttt cagccacggg
    38401 cgcggtctga acgtcgggca gttcgacggc ggtgccgtgt ggcagcaccg cccccagttc
    38461 agccagtccc ggatttgcgg cgagcacggc ctcgaccacg ccctcagtgc gcccgtaata
    38521 ccgaacacaa atggcgtcga gcgtgtcgcc ctgtagcgca aaggtcttca tcagatttga
    38581 ctcacgatgc agcgcggctt gtcctggata cgcgccaccg cccagcgcat atcccgccac
    38641 agctcatcaa tggtgctgtc aatgctgtcg gccttcttgt cgcccttggc actggcatcc
    38701 acgccgcggt aacgctcata aagcgacgcg gtcgccatcg cacacacagc gcgctcgtag
    38761 taaaaaactt tgatgctttc accgtcgatg tcgtccgccg ggacgtcagc cagacgcgaa
    38821 aaaccggcag caattttctg ttcgcggtac tcgtacagct ccgcattcgt ctccgccatg
    38881 cctgacttga tggcctcacg cagacgggcg ggggcgacgg tctgctcaag gcgcatacgt
    38941 tcccggacgc gcttcgggtc aatatcggga aaaaagaacg tgtttttaat caccggctcg
    39001 tcgcctgccg gttgcgggat aaccaccgta ccctcaccgg acacgggagc ctcctttcgc
    39061 ggaataatca gcgtcatcat gactacctct gaaaagtcgg gcggtggacg ccggtgcagt
    39121 gtcaggtgat tcaccgtcac tgaccggcgt gccgccctgg cgcggggcgc attcggttgt
    39181 taactggctt tctttttcgg gcgtccacgt tttgccggtg tcacgctccg ggtcttacgc
    39241 ggagtgcggg tggccgcttt gggctgcggc tccggcttcg gtttcagctc ccgctccagt
    39301 cgttcaatct cttttttgac gcctgcctga cagtcgagct gtgtcgcacg ttgcaggtgt
    39361 accagcgcac ctgcggcatc accagcgtca cgcagaaaca gaccggtgat tttgtgcagc
    39421 tttgcgcgca cttcatcagg catgtcagcc gtggcggtca gttcgagggt gtccgtcagc
    39481 aggcggggat ccacagactc accggcagcg tgagcgcgca tggccgcaag cgccacctcc
    39541 tcggtgaaca tgtacggcgg ggtgcggcgg tgtttacccg gcatggtcag accgtacttc
    39601 agggcataac gggcaatctc cagcgcaccg gcaatatcgc cggtatccag acgccacagc
    39661 atgaccgtca tcagaatgtc atcctgtgca cctttgccct gctccagcac gccgttcacc
    39721 cacggcaacc agaacggcag cagttcgcgt tttttcgcgg ccttcagctc ttttgaataa
    39781 atcgctttca gtgtgcgctg gtctgcggcc agcttgacca gcatctgctc atagacagtt
    39841 gcatgtcgca gcggggcggc ttcccgctgc gcggtcatcg ctgccgagac ccgcatcatg
    39901 tggcgctgtg cgggactcgt catcggttac gctcccggct ctgcggtcgc tttagccggt
    39961 gtggagaaat caccgacctt aattttttcc accagacaac cggcggcgta gtcctccacc
    40021 acgtaatcaa tgttcattga ctcgtagttc tccacgcggt cgagtttcgg gttttccaca
    40081 atcacgcggc gatggctgtc atccatgtag tagatggaca ggttttccag cttcgtgatg
    40141 agcatcgcat ccgccgggaa gtacgggacg cgtaccgccg gcaggttacc gatgcgtttc
    40201 tggctgatga tgacgtcagc ggccagcatc tcgctgttat cctgctcctt gttgacgata
    40261 gggaaatact tgtccgccag tagctgacgc cccacaatca ccacaaggtc agggtcttcc
    40321 tgataccaag gttcaatcag gttgttggtc gcatccatca ccagtgcatc gaggcttaca
    40381 taatcaccgc ccttacccac gcggataacc tcagaggtgg tgcggccttc ctcgtcagtg
    40441 accttgctca tcacgcgcgc cggggcttca ttcggtattt ctgcagccag ccgaccgcca
    40501 catcctgcag catcggatta ctgttgcggt cagaggtttc ggcacgcttc acgccgttaa
    40561 aaccggccat gatgaaatca agggactggc gtttgataat ggcgttacgg atacggagct
    40621 ggaaatcctg ataacgcgcc cacaggtcaa gcgttttgta gcggatataa aaatcgaagt
    40681 taacctggtc gcattcgtac ttgtttgacg ccagcttcga gaagtccttc ggctgacgct
    40741 cggtgccacc ggcggtgtcg gtggtgctgg cgatggagcc ggtgacaccg atgccaattt
    40801 tttccccttt catttcactg accggcacaa tgttgatgcg ggtcagaaag tcagaggact
    40861 cctgcatggt gttcatcagg gtctgggtga ccgacggttc aacggtgaat tttttcgaca
    40921 catcaccggc gtcgatgccg ttcagctcgg caacacggga caggtaagca ttaaatttaa
    40981 agcgggtttc ctggcgcata gtttttcctg aaattaaggg ttaatcgtga aggttttccc
    41041 ggactgactg acgccggtca gcagttcgtc atcagggcgt caccgccacc accggtggcc
    41101 ttgctgcggc gctgctgggt cagactttcg gtgtggtcga gactgttttt caggcgggtg
    41161 aatgcctggc tggtttcatc cgccctgtca gtcacctcct gcttaagtgc agaaaaggcg
    41221 gtttccatct cagcgaggcg ctgctcagtg gcgctcagtt tttcctgcac atgttcagca
    41281 acagcggtca ccgcttcatg cacgtcattc agacgggcat catcgctggc ctgtttgcgg
    41341 ccaaaaatgg atttcacctt ttcggtcagg gcggtgaaca cggtttcagg caggtcttca
    41401 aattccagct caacaggcgt tgccactgaa atcaggtttt cagggcttaa tttgaagcgg
    41461 ttcagggggt tgtgttttgc cgtgcggcag aattccaggt attccgtgcc gaggcttgcc
    41521 gggtcatcgg tgacggccag acccaccaga taacatttgc cggtgttggc aaagttcggc
    41581 tgaatttcca ttgaggtgta gaccttctgc gcggccttgt tcatcgcgat aaggtcatcg
    41641 gtcggggtga ttttcgcaaa cagcgcccat ttgcctttca gcgccgaatc atcgtcaatc
    41701 ttttcggcct tcagttcggc cacatcgcca taacgcttaa aaataccgtc aggcaggatg
    41761 ccgcgcagat gttccaggtt aatgcggcaa ccatagacac gcgggtcaaa ggtttcggcc
    41821 atttcctgaa tatcctgcgc actgatgaca cgcccgtcac aggtgtcacc ctcaacgccg
    41881 atacgaaaga attttgagac tttttttgcc attgtcagga gtcctgaata gtgattagag
    41941 gagtcacatg tcggcatcag tttcccgacg atgcgcatcc tccgccatca gtcccggatg
    42001 gtttatcact gacacaacag cacattagcg aatcgcgggg cgcgactcag tagccttgcc
    42061 gtgtattcat cacggcgagg tattcatgac catcaccaca gacaccactc ttttacacga
    42121 cccgcgtcgt caggcggcgc tgctgtactg gcaggggttt tccgtgccgc agattgccgc
    42181 catgttgcag atgaaacgcc cgacggtgca gagctggaaa cagcgcgacg gctgggacag
    42241 tgttgccccc atcagccgtg tcgaaatgag tctggaagcg cggctgaccc agctcatcat
    42301 caaaccgcag aaaaccggcg gtgacttcaa ggaaattgac ctgctcggac gccagattga
    42361 acgactggca cgggtaaacc gctacagcca gaccggcaac gaggcagacc ttaatccgaa
    42421 catcgctaac cgcaacaaag gcgggcgtcg caaaccgaaa aagaattttt tcagtgacga
    42481 ggccatcgaa aagctggagc agattttctt tgagcagtct ttcgactatc agttgcactg
    42541 gtatcgtgcc gggcttgagc accgcatccg cgatatcctg aaatcccgcc agattggcgc
    42601 gacgttttat ttttcccgcg aggcgctgct gcgcgccctg aaaaccggcc ataaccagat
    42661 ttttctgtcg gccagtaaaa cgcaggcgta tgtgttccgt gaatacatca tcgcctttgc
    42721 tcgtctggtt gacgttgacc tgaccggtga cccgattgtc ctgggcaata acggcgcaaa
    42781 actgattttt ctcggcacca actccaacac cgcacagagc cataacggcg acctgtacgt
    42841 cgacgagatt ttctggatcc cgaattttca ggtactgcgt aaggtggcat caggtatggc
    42901 ctcacagagt cacctgcgct cgacctattt ctccaccccg tccacgctgg cgcacgacgc
    42961 ctacccgttc tggtcgggtg aactgttcaa ccggggacgc gccagcgccg ccgaacgtgt
    43021 ggaaatcgac gtcagtcata acgcccttgc cggtggtctt ctctgtgcgg acggccagtg
    43081 gcggcagatt gtcaccattg aggacgccct gaaaggcggc tgcacattgt tcgacattga
    43141 gcagctcaaa cgcgaaaaca gcgccgacga ttttaaaaac ctgttcatgt gtgaatttgt
    43201 tgacgacaag gtatcggtgt tcccgttcga ggagctgcaa cgctgcatgg tcgacacgct
    43261 ggaagaatgg gaagactacg cgccgtttgc cgcaaatccg ttcggctccc gcccggtatg
    43321 gattggttac gacccgtcac accgtggcga cagcgccgga tgcgtggtgc tggcaccgcc
    43381 ggtggtggcc ggtggcaaat tcagaatact tgagcgtcac cagtggaaag gcatggactt
    43441 tgccacccag gcggagtcca tccgcaaact caccgaaaaa tacaacgtcg aatacatcgg
    43501 tattgatgcc accggcctcg gtgtcggcgt gttccagctc gtgcgctcgt tctatcccgc
    43561 cgcgcgcgat atccgctaca cgccggaaat gaaaaccgca atggtgctca aggccaaaga
    43621 cgtcatccgc cgtggctgtc tggaatatga cgtcagcgcc accgacatca ccagctcgtt
    43681 catggctatc cgcaagacca tgaccagcag cggacgcagt gccacctatg aggccagccg
    43741 cagcgaggaa gccagccacg ccgacctcgc ctgggcaacc atgcacgccc tgttaaatga
    43801 gccactcacc gccggtatca gcactccgct gacatccacc attctggagt tttactgatg
    43861 agcaagaaaa aagggaaaac accgcgacct gcggcaaaaa caatcaccgc cagcgccccg
    43921 aaaatggaag cattcacctt tggtgagccg gtgccggtac tcgaccgccg tgacattctg
    43981 gattacgtcg agtgcatcag taacggcaga tggtatgagc cgccggtcag ctttaccggt
    44041 ctggcaaaaa gcctgcgtgc tgccgtgcat cacagctcac cgatttacgt caaacgtaat
    44101 attctggctt caacgtttat cccgcacccg tggctttccc agcaggattt cagccgtttt
    44161 gtgctggatt ttctggtgtt cggtaatgcg tttctggaaa agcgttacag caccaccggt
    44221 aaggtcatca gactggaaac ctcaccggcc aaatataccc gtcgtggcgt ggaggaggat
    44281 gtttactggt gggtgccgtc cttcaacgag ccgacagcct tcgcgcccgg ctccgtgttt
    44341 cacctgctgg agcctgatat taatcaggag ctgtacggcc tgccggaata tctcagcgcc
    44401 cttaactctg catggctgaa tgagtcggcc acgctgttcc gccgcaagta ttacgaaaac
    44461 ggcgcacatg ccggatacat catgtacgtc accgatgccg tgcaggatcg caacgatatc
    44521 gaaatgcttc gcgaaaacat ggttaagtcg aaaggccgca ataactttaa aaatctgttt
    44581 ctctatgccc cacaggggaa agccgacggc attaaaatta tcccgctcag tgaagtggca
    44641 acgaaggacg atttttttaa tatcaaaaaa gccagcgccg ctgacctgct ggacgcgcac
    44701 cgcatcccct ttcagttgat ggggggcaag ccggagaacg tcgggtcgct gggtgatatt
    44761 gagaaagtgg caaaggtctt tgtccgcaat gagcttatcc cgttacagga caggatccgc
    44821 gagataaacg gctggctcgg tcaggaggtc atccgcttta aaaactactc actggacact
    44881 gacaacggct gaacatcgcc gcctgcgggc ggctttttta caccccgcca tcacgccctc
    44941 acacgctcat caccgcacaa aaaatcccgc agacacacca acgcccaacg ggcagactaa
    45001 gcgccgtcac gacgcgctga gacgctggaa aataaaatca gcaccaccgc cagcgcgcag
    45061 tgctttcccc gcctcgcccg cccgcttcat ggggcggttt taatgcagtt gcatgaacac
    45121 tccaagagca cgccagcgct agtgataact aacaataaaa gcgacttctg acgcatgcaa
    45181 atccatgcac atgatggcat ttttcatgat ctatgcggta tgctatccgc ttcaattttt
    45241 aacacttgtt aagggaacta gacatggaca ttattgattt aaaatctcaa ctagaaaatg
    45301 aagataatgc caagaaaaga gcagagttgc ttgctcagct agaggtgata aagcaaaacg
    45361 ctgaagagca aattcgtgct gagcaaaagg atgtggattt tgagaccaaa gaatttaccg
    45421 ttgagttatt agttaataag tatcataacg gattagagga tgatactaac gaactctttg
    45481 ttcctgatta tcaaagggat tttgtttgga gtgaaaaaag acaatcaaaa ttgatagagt
    45541 ctttgatttt gggttttcct ataccatata tatttatagc agatgttctc tccgaagatc
    45601 ccgatttaga tgggcgaatt gagattgttg atggctcaca acgagttagg actatacacg
    45661 cttttatcca aaatgaatta gttttacaag acctgaaatc tttagacgtc cttaatggtt
    45721 tcaaattcca agatttacca ttatcacgtc aacgtcgttt catgagaatt cctgtccgcg
    45781 ttatagaatt gagttctaaa tgcaatgaag aaacccgtag agatcttttt gagcgtatta
    45841 actctggcag cgatatattg aaagatatgg aagtccgtaa aggatcagaa cttggttcaa
    45901 caaatttata tacacaggtt attaagccat gctcaacaat acctaaattt aaagaattag
    45961 cacctttatc tcagactaaa gaaaaacgag atgaacggtt agaatttaca ttaagattct
    46021 ttgcctacgt agataagtac aagaactttg atcattccgt acgggatttt ctaaatgact
    46081 atatgtcaga aaatcgagat atagacgaaa ctaaacaaaa ctcaatgaaa caagagtttg
    46141 ctaatgttct atcttttgtt gaaaactatt tcccagcagg attcaagaaa actattactg
    46201 caaaatctac acctcgagtt agattcgaat ctctcgctgt tggcgcagca cttgcattac
    46261 gtgaagtccc tgaccttgtt cccaagaact tagattggct aagctcagaa gaattcaaac
    46321 gattgaccac ttccgacgga gctaatagtc gcgtgaaggt aattgaacga attgaatatg
    46381 ttcgggataa attattagag gattaacatg gatcgttttc gtgaggattt tgacgaaagg
    46441 tcaggagaga tcttggccta tttggatctt ttgaaattta ttgagtatgc cggtgcagaa
    46501 ctgatatcag ctgatgataa agaacataag ttctccatta ctgctcaatc acgaaaaact
    46561 ctaaagggag cagtatatat actgttatat aacctcattg aatcaacaat gagagaagct
    46621 atctgcctta ttcatgagac tatttatgat agaaacgtag aatttgacaa actcaggaaa
    46681 aatctcaggt cagaaatatt aaaaagatta aaaaatgaat ctgtaaatgt tgaaagttta
    46741 gttaatcacc ttaccaaagg tatatcttgc ggtatatctt atggcacgtt caacaaaaag
    46801 aaaatattct caggaaatat tgatagagag gaaatcaaag aaaaatcgaa aatatatggt
    46861 ttttctacac attccgatta cactcacact aaacatggtg aaaaattagc cactgttaaa
    46921 caacatcgta atgatttatc tcatggcaat gtatcatttg ctgagattgg aaaaaatgtt
    46981 tcatatcagg acttagagaa tatttcatta gaagtaatcg cctatcttga tgctatcgtg
    47041 aataatatag agcactatat aaataataat gaatatttag aacaatagtt ataaggcctg
    47101 taaagcaggc cattttatca ttctaaatgg ttcttgattg attgcccaat tgcttttcct
    47161 agttccacag gaacagcatt acctatcatt ttcccaatac ttcttataac aaacttgttt
    47221 ttgtcttcta caaaaatata atccatcggg aaagtttgca ataaagcagc ctctctaaga
    47281 gaaatcgctc tatgctgttc tggatgtcca aaacgcccat tcccgaaacc ataacaaaga
    47341 gttgtaattg ttggacttgg cttatcccaa gacatgcgcc catatacact agcataccct
    47401 ttaccgctgg atttcatatg acaagcggca accaactctt tcggccagtc cttccatgta
    47461 ccaccaggaa cagagtgtat aattcttttt ttattaatcg gatttaattt actggctcta
    47521 tgtaatatat catttggatc tgtttggcca tcttctaatg gaggaagatt tcctattgca
    47581 tctctaactg ttttcaaaat aacatcgtca cgttttacta actcaatcgc ccccaatttt
    47641 gaggccaaca atacatgacg ggaacggttt tgtgggattc catattcgac acaatcaatc
    47701 ttagacgccc aaacataata gcccaacttt aacaatgaat tataaaaatc attataaact
    47761 ttatgtttag ttacatcagg aacgttctcc attgttacga tttctggaga tacttcgcga
    47821 attaaacgtt cgaactcata aagaagaggc cattttttat catcagcctt atcttttcct
    47881 tgagtatatt ttgaaaaagg ttggcaaggt gcgcatcctg ccaatacttt tacagacgca
    47941 tcaccataaa gcctcagcag ttcctctttt gttactttag cgatatcttg ctcaataaac
    48001 ttagatttat tgtttctttc atagggaaac ctacattccc cctcaaggtc aatgcctgca
    48061 acgacatcaa gccccgcaag ctgcaaacca tgagttaatc cgccagcacc acaaaaaagg
    48121 tctatcacct ttacagtcat cactcactcc aaatctatac gatactcagt atacatccct
    48181 ttctacaaaa tctcgaggcc taacttataa actcatattt attgagttaa cgcttcactg
    48241 agtttgactc taatactgtt gagaatcccg accactcatc agcgagcggg taagtgaatt
    48301 ttttcccgtc ataatttacg gttgccccac gcgccagcac ctcaagctcc catcgctgcg
    48361 gcctgatacc gttctgagca aggtcaacgc ggatacgggt aatttgcatt cgttccgacc
    48421 gggtcagtct ggccgatggc gctatttcat gcggttttaa cgggcttccg tttctttgct
    48481 gacggtttgg tcttctcagt ccgtgtttta atgcacctct gagcgccctc acaacctccg
    48541 ggtcattcca ttcgataaca ccgtcatcta ccagattaag cactgctgcg gcgtgctcag
    48601 aaggtgtggg agccggtaac gaagtatcac caccagtgag ctttccacag ttattgacag
    48661 gactccgagg cgcggcgatg ccgcttttta aagtcaaagg ctcaacgacc ggcactttcg
    48721 gaacaatgcg ccagtccgtc gttctggtga tatgaatatg acgcgcgccg agatgcggcg
    48781 cgtaaatgcc gaccactctc tcgacttctt cctcgtactc gttaacttca tccgacggac
    48841 tacgggcgac cctgacagtc tgacaatcgc gcgggacatt tgccccaccc tgcgcgctga
    48901 tatacagcgc aaaatcacca ctgtctgcgg cggcgcgtgc agcctcgacg cgctcgtcaa
    48961 actcatcagc aatgctgacg ccgcgaggca atttgcgtag ttcacggtaa gcccccattg
    49021 tcggcaggcc aaccgtttta aattgcggaa tgcgccacgt tgacgcccat gcggtaacag
    49081 ccgcggcagt atctttcagc ggtctgccgc tatcgttatc gagctgccca tccagtgcat
    49141 agccgtcgat attttttgag atgtatttcg cgatataccc cgcagcaccg ccccgattaa
    49201 gatgttttgc ctgaaaacgg tttcgcgcag ctcctctttc gtcgccatcc tctttgagcg
    49261 cgtagcgacg catgatttcg ataatctggt tacgctggcg tggattacaa aaaagcatca
    49321 tatgccagtg cggcgttccg tcgtggtgtg gctcgacgac tcgcaaaccg tagacctgta
    49381 aatcattatc cttgaatgcc gtgcgcatca ggctccagat acggcagaga taacgctgcg
    49441 catcctttgg attaaatgcc tcatcgttcc agccgtgatt tagctggacg gttttacttt
    49501 cgccttttcc aacctgacgt gtcgggtgat actttgacgg cgcggtcagc gtgataaaca
    49561 tccccacatc accttctgcg gcggcgtaac gctcaatacc ggcaatggtg ttcatcagct
    49621 ccatccggcg aatttcagga ttagaaatac tgcccatcac cttactgata aggtcgatgc
    49681 gctcgcctgt ttccctgttt tcgagatcac acgatttaag aaattccaga tttgcctggc
    49741 ggcgtgcacg cacatcacga atggcatgtt tactggcata aggagaacgg tctttattga
    49801 cctccccgac agcaatcagt aacgcctcat gccagcgcat acgctggcct ttaagctgac
    49861 taatccacca ctcatcgtta aacagacggg caatggcaga atatgcctgc ctcgtggtca
    49921 tctgtccttt acggtatttt ttccagtaga gaggggaaat attgaaagca cgtgcagcgc
    49981 cagcaacatg accatagagg tgagcctgcg cctcatccgt aaacagcgat tctttttcgc
    50041 catgcgcatc cacccaggca tcgctgagtt cctcatacat catgaaaagc tgcgatgaga
    50101 tacgggcggc aaactttttc agctccttgt cattcattcc cggcaggcgc gcatagtggt
    50161 cacgctcagc cagaaacagc atcgacgcgt cggtgttcat ttcatggcgc tgattcacac
    50221 gctcaatgcg cggccataaa cgacgctgaa aagtggatgt gaggaaataa aacccgtgca
    50281 ccgggctttt attgcgccgg atgtagtcat agcgtgacgt aaacagcgag cgcaaaaagt
    50341 aaggcaggcg gttaatcgtg gataaaacac cttgcacctg acgcatctcg tcacgtgtaa
    50401 ggggtctttc gcgcccgaca gcctcgcgtg gcgcgttcca tgcataagca ccggtaaacg
    50461 ccttaccggt gcctgcagca aatgctgacg gagggacaaa acgcccggag gctttaacgg
    50521 ccatatgagc caaaagcctc tgaacaacgc ttgctgagtt gctcaacctg cgcgtttaaa
    50581 tcagcaaaag actttgcgct tccggtcaga atatcgtgat gcatcaggcc ggaaacgagc
    50641 tggcttaatt tcgggtaata accaaccacc gccagccatt cctgaccggc gtttttaccg
    50701 ctttccgctc tctttttctc gtggagaata aactggaagc tgtcactggt aacgacataa
    50761 cgttcgccaa tttcaatacg aatactcatg ccgttctccg gtaatgtttg ttttttgctt
    50821 caaagactga ctgacaggaa acacaacgcg tggctgacgg ataagccgca cgacgggcag
    50881 caggaattgg cgcgtcacac tcttcgcaaa ccagcgcaga agcaccgcaa tgttttaccc
    50941 ttgccgcgtt aatctggcgc tccagtaatt cagcctgttg ttcctgaata aaatctatgt
    51001 tgtccggcat tatcagctcc ttttatcgtt aagtttcctg gatacatcag cgcaataact
    51061 ggcaagttct gtcgttaatt ttgtcagttc atccactgag gaaatttgct tgtggaatac
    51121 agcgcgttta acaagtaaat tgaccacatc agacaggagg tttaattcat tctgataaat
    51181 cgcgataaca gattcagtga tgtcgcgttt ttctttatca agacaaagtt gaataagaga
    51241 caagtcgcca tttttcataa cggcgatttt taaggcgtta ttcagtaata caactgaatg
    51301 agaacaggac atcaaagcac ctccccgcga gacaatccga tattgtgaaa tttttccgac
    51361 tcctgactga gcagctcgac tatctccacg cgggataact ccgcctttgt gatatggcga
    51421 atcatggcgt caagatgaga agaaaagcgc gtcgcagcgt cggcctgtgc ttcggttctg
    51481 gcctgttgca gcagtaatgc gtatttaccg cactgatttt cagaaactgt atgcatgact
    51541 ttctccaggc aaaaagaagc cccgcacaat taagtgcgtt aaaaactctg gttaattatt
    51601 taatgcaaat attgctctgg ttttaccgac gtcagaattg tcggtgcata ctcaaacaga
    51661 ctgaatagtt cacgtaatgc acggaataaa gcatcacgcc agtaacatga ctcttcatta
    51721 attcgccagt atggctgatt gaattctttt tcagtcaatc cggcatgcat aaataaagta
    51781 cgacgctgac tgactgttaa aaaactaata tatgcatact cacttgcgcc aacctgacgg
    51841 cgttttgaga atgccccacg caattcatca attgcacaaa ccagccgttc acgttcgacg
    51901 tcgttcattt cttcaaaacg catcgttgcg tgacgttgtt ttaactgcgc atgaaagcaa
    51961 actgttagcc gttcgcgctc catcatctga ttataataat cacatgtatc ctgccaacga
    52021 gggacggcaa gatgcttacc aattatccgg cgcatagctg ctggctgttt ttcgacgaga
    52081 ttgagcgtca tcactgtcat ttccagaccc tccggctttt cagaaaggtc agagcctttt
    52141 ttaacggact ctgttttttg gtgcggataa tgattccctt gcgacccttc ccgtgggtga
    52201 tggtgaagtc aatcgcccta gggctttcgt tacgcaataa ctgagcaata caacgcggct
    52261 cattcataat cacaacccca tccacaaaag ccatgcatca cgctgttcaa ccggtcggtt
    52321 ataaaacgcc tcacgtacag cgcgattaaa ctcaggaatg aaaacccatt tttcaccggc
    52381 acgagccttc ggtttgcaag gatcacgcaa ttcaataatt ggtaatttat ttgccttcac
    52441 catttcactg acggctgtct ttggcttccc taataaatca gcaaatttat ccacatgaac
    52501 cgcatcaagc ggatactgaa tcacataatt ttcagcgtcc atatatggta ccctcatagg
    52561 atccagccct ttctaaacca ctcaaaaccg tttagacgct ggtttattct caaatcaatg
    52621 gaacctatat aggttccagt tttgagggaa tttagtccct atataggcac catgtcaaat
    52681 gaaattaagc gaaaagatta aggccttgcg tgaggctgaa gggctaagcc aatcaaaatt
    52741 ctgtgaaatc atagagttac cgctaagcac acttaaaaaa tatgaaggag gaaactttga
    52801 acccggtggc acagctttgc taaaaatcac tatgcatccc acattccaaa aatatgctct
    52861 atggcttatg acagataaaa ccgcgccgga cgcaggacaa atcgcaccgg ctctcgcgca
    52921 cattgggcca gagtcaacag agtccaacca ctccgcgaaa aggattggct aactctatat
    52981 aaagattaca ttttcaccat ttgctaccaa gatggtgaat acagcgccgg agggctttct
    53041 tatggcaatt aagaagctcg atgatggtcg ctatgaagtg gacattagac ctcgcggtcg
    53101 cgacggaaaa cgcatccgca ggaaattcga aagaaaagct gaagcactag catttgagcg
    53161 atacacaatc gccaatgcca gtcagaaaga atggggaggc cagcgagcag accgccggac
    53221 tttgagtgag ttgctggaca tctggtggaa atatcacggg caaaaccacg agcatggaac
    53281 aaaagagttt aatcatctac tcaaaaccat cagcggcata ggtgatatac cagtgagcag
    53341 gatgagcaaa agggctttga tggattatcg ttccatgcga ctacgtgatg gtatcagtgc
    53401 cgcaacgata aaccgtgaca tgtacagatt atccggcatg ttcacaaaat taattcaatt
    53461 ggatgaattt tccgggcaac acccaattca cggactgccg ccactggcgg aggccaaccc
    53521 tgaaatgacg ttcctggaaa aagcagaaat cgaaaaactg ttaaatgttt tggctggtga
    53581 tgacttactt gtcgcgcttt tatgtctgag cactggagga agatggacga aagttgccac
    53641 gctaaaacca gcacagatta caaattgcag ggttaccttc ctgaaaacca aaaacggtaa
    53701 aaagcgaacc gtgccgattt ctgaggaact ggagaaaaaa gttaaagagg aggccagcgc
    53761 caaattattc aaagttgatt atgagaaatt ttgcgggatt ttacgcagag tgaaacctga
    53821 tataccaccc aatcaggcaa cccacatcct gcggcataca ttcgcaagcc atttcatgat
    53881 gaatgggggc aatataatcg cactgcaaca gattctggga catgcgagca ttcagcagac
    53941 gatggcctat gcgcaccttg cgcctgacta tctgcaaaat gccgtcgctc tgaatccact
    54001 aaaaggcgga gtgacgttat aaatttcccc ctctgagtgt ccacatagtg tccacactca
    54061 agaaattttg aaaccgctcc aaacccttac agctttcgat aagtaactgt ttttactttt
    54121 gattgcatgt aagtatttga taaaaaaaac cccacatcat gtgggggaag acagggatgg
    54181 tgtctatggc aaggaaaaca gggtttacta ctgggaacgt gagttgctac tactcaatag
    54241 cttcaacgat gaactttttt gccattgcgt cacgtcacgc aactgctcca ttcgttgttg
    54301 atgtttctcg tttaaaaccg cttgctgctc cggcgttaac aggcgataca tttggttgcg
    54361 gacttttgcc atctcaacct gacgagcaat ttgctcattc gccatttttt ctgcctgtgc
    54421 gcgcacagcg ttttcatcaa aattttctgc ggtgacaagg cgatgcattg tctccagttc
    54481 gctaacatta acaggaggct gttcgtgccg ggcctgttgc ataagatctc gcatctgctg
    54541 acgctgatgt tcggttaaac ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt
    54601 aagttcttca cccggatgcc agttatcgcc tgaaccgact tcagcagcgt ggcttaatga
    54661 actgactgcc agcgttgagg ccatgacggc agcggtaact atgcgcatca tttgctccca
    54721 aaatctttct gtcgcgattc aacgatagag agtttacgat tcaggctgca aacatgcgtc
    54781 agggggtgta aaacaacgta aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc
    54841 tcggaggtat ttaaacaatg aataaaatcc tgttagttga tgatgaccga gagctgactt
    54901 ccctattaaa ggagctgctc gagatggaag gcttcaacgt gattgttgcc cacgatgggg
    54961 aacaggcgct tgatcttctg gacgacagca ttgatttact tttgcttgac gtaatgatgc
    55021 cgaagaaaaa tggtatcgac acattaaaag cacttcgcca gacacaccag acgcctgtca
    55081 ttatgttgac ggcgcgcggc agtgaacttg atcgcgttct cggccttgag ctgggcgcag
    55141 atgactatct cccgaaaccg tttaatgatc gtgagctggt ggcacgtatt cgcgcgatcc
    55201 tgcgccgttc gcactggagc gagcaacagc aaaacaacga caacggttca ccgacactgg
    55261 aagttgatgc cttagtgctg aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc
    55321 tggagttaac cggtactgag tttaccctgc tctatttgct ggcacagcat ctgggtcagg
    55381 tggtttcccg tgaacattta agccaggaag tgttgggcaa acgcctgacg cctttcgacc
    55441 gcgctattga tatgcacatt tccaacctgc gtcgtaaact gccggatcgt aaagatggtc
    55501 acccgtggtt taaaaccttg cgtggtcgcg gctatctgat ggtttctgct tcatgatagg
    55561 cagcttaacc gcgcgcatct tcgccatctt ctggctgacg ctggcgctgg tgttgatgtt
    55621 ggttttgatg ttacccaagc tcgattcacg ccagatgacc gagcttctgg atagcgaaca
    55681 gcgtcagggg ctgatgattg agcagcatgt cgaagcggag ctggcgaacg atccgcccaa
    55741 cgatttaatg tggtggcggc gtctgttccg ggcgattgat aagtgggcac cgccaggaca
    55801 gcgtttgtta ttggtgacca ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca
    55861 gatcattcgt aactttattg gtcaggccga taacgccgat catccgcaga agaaaaagta
    55921 tggccgcgtg gaactggtcg gtccgttctc cgtgcgtgat ggcgaagata attaccaact
    55981 ttatctgatt cgtccggcca gcagttctca atccgatttc attaacttac tgtttgaccg
    56041 cccgctatta ctgctgattg tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc
    56101 ctggagtctg gcaaaaccgg cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg
    56161 aaacttacgc cagcacccgg aactggaagc ggggccacag gaattccttg ccgcaggtgc
    56221 cagttttaac cagatggtca ccgcgctgga gcgcatgatg acctctcagc agcgtctgct
    56281 ttctgatatc tctcacgagc tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt
    56341 actgcgccgt cgtagcggtg aaagcaagga actcgagcgt attgaaaccg aagcgcaacg
    56401 tctggacagc atgatcaacg atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct
    56461 ggttagcgaa accatcaaag ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt
    56521 cgaagccgag caaatgggca agtcgttgac agttaacttc ccgcctgggc cgtggccgct
    56581 gtacggcaat ccgaacgccc tggaaagtgc gctggaaaac attgttcgta atgctctgcg
    56641 ttattcccat acgaagattg aagtgggctt tgcggtagat aaagacggta tcaccattac
    56701 ggtggacgac gatggtcctg gcgttagccc ggaagatcgc gaacagattt tccgtccgtt
    56761 ctatcggacc gatgaagcac gcgatcgtga atctggcggt acaggattgg ggctggcgat
    56821 tgttgaaacc gccattcagc agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg
    56881 cggtttacgg ctggtgattt ggttgccgct gtataagcgg agttaaactc cgcatttgta
    56941 ggcaggataa ggcgtttacg ccgcatccgg catttgagca ggatgcctga tgcgacgctg
    57001 atagcgtctt atcaggccta cactccgggg gttattttcc ccacaaccgg cgggaaaaat
    57061 cttcgatctt gccgctcagt cggcgctttt gcatcgtccg cgtccagctt ttcgataacc
    57121 cggcggcgga gagtaaacgg tgatactgat cgtcatcaaa cggcatatgc catgcgatgg
    57181 cggcagcctc ctgcacggtc acatcgctga cacgggaaac caactccagc ggcgcgtccg
    57241 cagacacttt ccctggcgct atcacgctat acaaccaccc caccttacct gtgttttgca
    57301 tcaactgcgc tatatcgctg atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg
    57361 gctggctgac ctgaattaat gcctctcccc agcggaaaat atcgcccata tgaacattac
    57421 tttccgtgag accgtcggtt gagagatttt cgccaaacgc aggcgcaaca aacaactccg
    57481 cctgttccgg aaattcccgc acccagtaga gatagtgctc acgaggataa tgacacagcg
    57541 ctctgtctgg cccgccgtga acctttttct ccgcctgctc gtcaccttcc agccccagct
    57601 ccgtcagcat caactcacca tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg
    57661 gataagcctg aatcttgcct gtgtatacat caaccggata tcgcatctgt tccctctcca
    57721 cgagaaattt ccccattaga ccatatttat tttatgtcag gcgtgctgtt ttcgcggtaa
    57781 gtatccaggc aggaactcaa tagcaaaccg gacgaacgtt agttcatccg gctgtgggga
    57841 ttaaggaagc aggtttattt caccgtacct aaaatttcga ttttcgctgc tctggctttg
    57901 actttacgtg accagataga ggtaagaatt ggcaccagaa tcgaagtcac aatgaccgcc
    57961 gtcgctacca gcgaagttgc tgccggagcc atcggtttaa acgcaggcac catttctgca
    58021 atcagcacag gtgtcgctac cgcggcccct gcggaactgg aagcggcaat tccggcagtg
    58081 ccatcgccac cgccaatcaa tttatcggcg ataatcaacg gaataccggt cacgataatt
    58141 actgccacac ccaacaggat ccccagcaaa ccagtctggg caatcacagt caaatcaatg
    58201 gtattgccca gcgcgaaggc aaagaatgga atcagcgttt gcactgcttt gctgaaaaat
    58261 tctcgtaatt cagggtcaag attccccagg gcaaagccca ccaggaacgg taatactgcg
    58321 ccgacgaaaa catgcggttc aaacgaggca atcccggcag tgcccagaat aatcatcgtc
    58381 atgagcggcc cggactccaa cgacatcaac acaaatgccc cagcttcttc tttcgtaccg
    58441 tactgctgca tgatggaagc gtaaagtccg ccgttggtca tatccatcgc cgccaccagc
    58501 gccagcgttg aaagtccggc aaagaatcca acttcaacac catgttccgg aataatgcgc
    58561 gaggcaattg ccgcaaccac ccacgcgacg gcaattttag ttaccaccag cgtaccggat
    58621 ttacgcagta ccgttcccgt cgcgcttaat tttattgacg cccccatgca aaaaaaccac
    58681 accgccagaa tgggcaccgt accggtaatc atcccgttgg tgaatgatcc aaaatatttc
    58741 cccgcccccg gcgagaaggt gtggcacagt gcgccaagga atagcgggac gagcatcatc
    58801 ccccccggga ttttctcaat cgagcgtttt atctgcattt ccatcacctg tcggcatatt
    58861 tttaagggct gatgcgtaat tcctcaatgg agcatcagtg ttgatggaaa cgaagataga
    58921 cattcattta atcaataaaa gtgatcaaaa ccacatcaat tgaaacgctg ttttattttt
    58981 atcggatcat tgtttttatt ttgaaattga tcacaaaaaa accaccgccg ttggcgatgg
    59041 ttcatttttt aagctgatat gcggcctata cgcctcattg cagcaggcgg caaatgatta
    59101 ttttttcgcc gcaaaacgtg ccgctgcttc gtcccagttc accacgttcc agaactcttt
    59161 aatgtagtcc ggacggcggt tctggaattt caggtagtaa gcatgttccc acacatccag
    59221 gcccagaatc gggaagccgg aagcgccaga aatagcttca cccatcagcg gagaatcctg
    59281 gttagcagta gaaaccaccg ccagtttatc gcctttcagc accagccatg cccagccgga
    59341 accaaagcgg gaagctgccg ctttttcaaa ttctgctttg aagttatcaa cggagccgaa
    59401 gtcacgttcg atagccgctt tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt
    59461 ccagaacagg ctgtggttag cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc
    59521 tgctggcagc tggtccagtt tggtgatcag ctcttcaacc ggcaggttgg caaattctgg
    59581 caggctttcc agcgccgcgt tggcgttgtt tacgtaggtc tgatggtgtt tggtgtggtg
    59641 gatttccatg gtctgcttat cgaagtgcgg ttccagggca tcgtaagcat acggcaggga
    59701 tggcagggta tagctcatat tcatctccag tattgtcggg cggccgattg ttaatgccgc
    59761 gtaagcagtt ggttcattat agttaattaa atgatattga aaatgattat caatgccgta
    59821 cttttcgtaa gggtatggtt ttgcaggaaa atgcccgaga tgtgaagcaa atcacccact
    59881 taatgccgtg attgccagta aatcgacaac ggcggcaaca ggcgaaaggt taatcgacag
    59941 cacgattttt acactcatct cgtcggagat gtgaagcgac gaaaaatgat gaggataaga
    60001 agatgagtaa cgcgattacg atggggatat tttggcattt gatcggcgcg gccagtgcag
    60061 cctgttttta cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc atgtggtcag
    60121 tcggtgggat tgtttcgtgg attattctgc cgtggaccat cagcgccctg ttactaccgg
    60181 atttctgggc gtattacagc tcgtttagtc tctctacgct gctgcctgtt tttctgttcg
    60241 gcgctatgtg ggggatcggt aatatcaact atggcctgac catgcgttat ctcggcatgt
    60301 cgatgggaat tggcatcgcc attggcatta cgttgattgt cggtacgctg atgacgccaa
    60361 ttatcaacgg caatttcgat gtgctgatta acaccgaagg cggacgcatg acgttgctcg
    60421 gcgttctggt ggcgctgatt ggcgtaggga ttgtaactcg agccgggcag ttgaaagagc
    60481 gcaagatggg cattaaagcc gaagagttca atctgaaaaa agggctggtg ctggcggtga
    60541 tgtgcggcat tttctctgcc gggatgtcct ttgcgatgaa cgccgcaaaa ccgatgcatg
    60601 aagccgctgc cgcacttggc gtcgatccac tgtatgtcgc tctgccaagc tatgttgtca
    60661 tcatgggcgg cggcgcgatc attaacctcg gtttctgttt tattcgtctg gcaaaagtga
    60721 aggatttgtc gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt cacaatgtgt
    60781 tactctcggc actgggcggg ttgatgtggt atctgcaatt ctttttctat gcctggggcc
    60841 acgcccgcat tccggcgcag tatgactaca tcagttggat gctgcatatg agtttctatg
    60901 tattgtgcgg cggtatcgtc gggctggtgc tgaaagagtg gaacaataca ggacgccgtc
    60961 cggtaacggt gttgagcctc ggttgtgtgg tgattattgt cgccgctaac atcgtcggca
    61021 tcggcatggc gaattaatct ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc
    61081 cggtttcccg ggtaaacacc accgaaaaat agttactatc ttcaaagcca cattcggtcg
    61141 aaatatcact gattaacagg cggctatgct ggagaagata ttgcgcatga cacactctga
    61201 cctgtcgcag atattgattg atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa
    61261 cgcgctcact gcacgatgcc tcatcacaaa atttatccag cgcaaaggga cttttcaggc
    61321 tagccgccaa ccgggtaatc agcttatcca gcaacgtttc gctggatgtt ggcggcaacg
    61381 aatcactggt gtaacgatgg cgattcagca acatcaccaa ctgcccgaac agcaactcag
    61441 ccatttcgtt agcaaacgac acatgctgac tactttcatg ctcaagctga ccgataacct
    61501 gccgcgcctg cgccatcccc acgctaccta agcgccagtg tggttgccct gcgctggcgc
    61561 taaatcccgg aatcgctccc tgccagtcaa gattcagctt cagccgctcc gggcaataaa
    61621 taatattctg caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa
    61681 tataaaagag atcgccacgg gtaatgcgat aaggacgatc gttgagtaca tgcagaccat
    61741 taccgcgcca gacaatcacc agctcacaaa aatcatgtgt atgttcggca aagacatctt
    61801 gcggataacg gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga
    61861 gaagttttaa ctgatgcgcc accgtggcta cctcggccag agaacgaagt tgattattcg
    61921 caatatggcg tacaaatacg ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc
    61981 tggcgaatat cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta
    62041 ctgtcgctga atccacagcg ataggcgatg tcagtaacgc tggcctcgct gtggcgtagc
    62101 agatgtcggg ctttcatcag tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt
    62161 tgctgcttaa gctgccgatg tagcgtacgc agtgaaagag aaaattgatc cgccacggca
    62221 tcccaattca cctcatcggc aaaatggtcc tccagccagg ccagaagcaa gttgagacgt
    62281 gatgcgctgt tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc
    62341 ataaacaaga tctcgcgact ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc
    62401 atctgtgcaa ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg ccagtgagac
    62461 ggatactgcc catccagctc ttgtggcagc aactgattca gcccggcgag aaactgaaat
    62521 cgatccggcg agcgatacag cacattggtc agacacagat tatcggtatg ttcatacaga
    62581 tgccgatcat gagcgcgtac gaaacagacc gtgccaccgg tgatggtata gggctgccca
    62641 ttaaacacat gaatacccgt gccatgttcg acaatcacaa tttcatgaaa atcatgatga
    62701 tgttcaggaa aatccgcctg cgggagccgg ggttctatcg ccacggacgc gttaccagac
    62761 ggaaaaaaat ccacactatg taatacggtc atactggcct cctgatgtcg tcaacacggc
    62821 gaaatagtaa tcacgaggtc aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa
    62881 aacgtcgatt tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac
    62941 cacaatgaag caaattgtga acatcatcac gttcatcttt ccctggttgc caatggccca
    63001 ttttcctgtc agtaacgaga aggtcacgaa ttcaggcgct ttttagactg gtcgtaatga
    63061 aattcagcag gatcacatta tgacctttcg caattgtgtc gccgtcgatc tcggcgcatc
    63121 cagtgggcgc gtgatgctgg cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga
    63181 aatccatcgt tttaacaatg gcctgcatag ccagaacggt tatgtcacct ggaatgtgga
    63241 tagcctggaa agtgccattc gccttggatt aaacaaggtg tgcgaggaag ggattcgtat
    63301 cgatagcatt gggattgata cctggggcgt ggactttgtg ctgctcgacc aacagggtca
    63361 gcgtgtgggc ctgcccgttg cttatcgcga tagccgctcc aatggcctaa tggcgcaggc
    63421 acagcaacaa ctcggcaaac gcgatattta tcaacgtagc ggcatccagt ttctgccctt
    63481 caatacgctt tatcaactgc gtgcgctgac ggagcaacaa cctgaactta ttccacacat
    63541 tgctcacgct ctgctgatgc cggattactt cagctatcgc ctgaccggca agatgaactg
    63601 ggagtacacc aacgccacga ccacgcaact ggtcaatatc aatagcgacg actgggacga
    63661 gtcgctactg gcgtggagcg gggctaacaa agcctggttt ggtcgcccga cgcatccggg
    63721 taatgtcatt gggcactgga tttgcccgca gggtaatgag attccggtgg tcgccgttgc
    63781 cagccatgat accgccagcg cggttatcgc ctcgccgtta aacggttcac gcgctgctta
    63841 tctctcttct ggcacctggt cattgatggg cttcgaaagc cagacgccat ttaccaatga
    63901 cacggcgctg gcagccaaca taaccaatga aggcggggcg gaaggtcgct atcgggtgct
    63961 gaaaaatatt atgggcttat ggctgcttca gcgagtgctt caggagcggc aaatcaacga
    64021 tctcccggcg cttatcgccg cgacacaggc acttccggcc tgccgcttca tcatcaatcc
    64081 caatgacgat cgctttatta atcctgaggc gatgtgcagc gaaattcagg ctgcgtgtcg
    64141 ggaaatggcg caaccgctcc cggaaagtga tgctgaactg gcgcgctgca ttttcgacag
    64201 tctggcgctg ctgtatgccg atgtgttgca tgagctggcg cagctacgcg gtgaagattt
    64261 ctcgcaactg catattgtcg gcggcggctg ccagaacacg ctgctcaacc agctatgtgc
    64321 cgatgcctgc ggtattcggg tgatcgccgg gcctgttgaa gcctcgacgc tcggcaatat
    64381 cggcatccag ttaatgacgc tggatgaact caacaatgtg gatgatttcc gtcaggtcgt
    64441 cagcaccacc gcgaatctga ccacctttac ccctaatcct gacagtgaaa ttgcccacta
    64501 tgtggcgcag cttcactcga cacgacagac aaaggagctt tgcgcatgac cactcaactg
    64561 gaacaggcct gggagctggc gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag
    64621 gcgctgcgcc aacttgatcg tttacccgtt tcaatgcact gctggcaggg cgatgatgtt
    64681 tccggttttg aaaacccgga aggttcgctg accggtggga ttcaggctac tggtaattat
    64741 ccgggcaaag cgcgtaatgc cagtgagcta cgagccgatc tggaacaggc tatgcggctg
    64801 attccggggc cgaaacggct taatttacat gccatctatc tggaatcaga cacgccagtc
    64861 tcgcgcgacc agatcaaacc tgagcacttc aaaaactggg ttgaatgggc gaaagccaat
    64921 cagctcggtc tggattttaa cccctcctgt ttctcgcatc cgctaagcgc cgatggcttt
    64981 acgctctcgc atcccgacga cagcattcgc cagttctgga ttgatcactg caaagccagc
    65041 cgtcgcgttt cggcctattt tggcgagcaa ctcggcacac catcggtgat gaacatctgg
    65101 atcccggatg gtatgaaaga tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg
    65161 gcagcactgg atgaggtgat cagcgagaag ctggatccgg cgcaccatat cgacgccgtt
    65221 gagagcaaat tgtttggcat tggcgcagag agctacacgg ttggctccaa tgagttttac
    65281 atggggtatg ccaccagccg ccagactgcg ctgtgcctgg acgccgggca tttccacccg
    65341 actgaagtga tttccgacaa gatttccgcc gccatgctgt atgtaccgca gttgctgctg
    65401 cacgtcagcc gtccggttcg ctgggacagc gatcacgtgg tgctgctgga tgatgaaacc
    65461 caggcgattg ccagtgagat tgttcgtcac gatctgttcg accgggtgca tatcggcctc
    65521 gacttctttg atgcctctat caaccgcatt gccgcgtggg tcattggtac acgcaatatg
    65581 aaaaaagccc tgctgcgtgc gttgctggaa cctaccgctg agctgcgcaa gctggaagcg
    65641 gcgggcgatt acactgcgcg tctggcgcta ctggaagaac agaaatcgtt gccgtggcag
    65701 gcggtctggg aaatgtattg ccaacgtcac gatacgccag caggtagcga atggctggag
    65761 agcgtgcggg cttatgagaa agcgattttg agccagcgtg ggtaaacact gccggatgcg
    65821 gcgcgagcgc cttatccggc ctacaggtcg aatgaccatg cgtgctgcca ccctcacccc
    65881 ggccctctcc ctgaaagggc gagggagaaa aacatagcct cgtgcggcaa cggagctgaa
    65941 caccgctccg gacagtcccc tcgcccctac ggggagaggg ttagggtgag gggaacaagc
    66001 cgcaccatgt ctaaacaagg caacctaatt tacaggaaca cagaacatgc aaaacattac
    66061 ccagtcctgg tttgtccagg gaatgatcaa agccaccacc gatgcctggc tgaaaggctg
    66121 ggacgagcgc aatggtggta acctgacgct gcgcctggat gacgccgata tcgcgcctta
    66181 tcacggcaac tttcatgcac aaccgcgcta tattcccctc agccaaccca tgcctttact
    66241 ggcaaatacg ccgtttattg tcaccggctc cggcaaattc ttccgtaacg tccagcttga
    66301 tcctgcagct aacttaggcg tcgtaaaagt cgacagcgac ggcgcgggct accatattct
    66361 ctgggggtta accaacgaag ccgttccaac ttccgaactt ccggctcact tcctttccca
    66421 ctgcgagcgc attaaagcca ccaacggtaa agatcgggtg atcatgcact gccacgccac
    66481 caacctgatc gccctcacct atgtacttga aaacgacacc gcggtcttta ctcgccaact
    66541 gtgggaaggc agcaccgagt gtctggtggt attcccggat ggcgttggca ttctgccgtg
    66601 gatggtgccc ggcacggacg aaatcggcca ggcgaccgca caagagatgc aaaaacattc
    66661 gctggtgttg tggcccttcc acggcgtctt cggcagcggg tcgacgttgg atgaaacatt
    66721 cggtttaatc gacaccgcag aaaaatcagc acaagtatta gtgaaggttt attcgatggg
    66781 cggcatgaaa cagaccatca gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt
    66841 tacaccacta gctagtgcgc tggcgctgta aggagcaaac atgatccgca aagcctttgt
    66901 catgcaggtt aaccccgacg cccacgaaga gtatcagcgt cggcataacc ccatctggcc
    66961 ggaactggaa gcagtgttga aatctcacgg tgcgcataac tacgccattt acctcgacaa
    67021 agcgcgtaat ctgctgtttg ccacggtaga gattgaatct gaagaacggt ggaatgcggt
    67081 agccagcact gatgtttgcc aacgttggtg gaaatatatg accgatgtta tgcccgctaa
    67141 cccggataac agcccggtga gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc
    67201 caatgtgcca gagatgcctc cggcacaata ttttaaattt cgatttgaaa aattaaacat
    67261 attacatctc ccggtaagtt atatttccct gatacattgt gagtaaatca caaaaataat
    67321 gaataaccca ttaatgattc atgtggttta tttaaataac cattatgtgc attactccgc
    67381 aaatctgacc tttcactctg tctcaaattg tcattgttta ccgacatatc gcctctcatc
    67441 acttaatcgc tccaccatta caattgatta aaatatattt tatggagtcc gattaatggc
    67501 agctcttact gcaagctgta ttgacctgaa tattcagggc aatggcgctt attccgttct
    67561 gaagcagttg gcgacaatag cgttacaaaa cggttttatc accgactcac accagttcct
    67621 gcaaaccctg ctgctgcgcg aaaaaatgca ctctactgga tttggttccg gtgtcgccgt
    67681 gccgcacggt aaaagcgcct gcgttaaaca accgtttgta ttattcgccc gcaaagcgca
    67741 ggctattgac tggaaagcca gcgatggcga agacgtcaat tgctggatct gcctcggcgt
    67801 gccgcaaagc ggcgaagagg atcaggtcaa aatcatcggc acactgtgtc gcaaaattat
    67861 ccaccaggat tttattcatc aactgcaaca gggcgacgcc gaccaggtgc ttgccttgtt
    67921 aaatcaaacc ctcagctcat aaggaagtgg cgatggagtc atccttatgt attgtcgcga
    67981 tcaccaactg ccccgccggg atcgctcaca cctacatggt ggcggaagcc ctggaacaga
    68041 aagcccgttc tctcggccat accataaaag tggaaactca agggtccagt ggcgttgaaa
    68101 accgcttatc cagcgaagag attgccgctg ccgattacgt cattctcgct accgggcgtg
    68161 gcctgagcgg tgatgatcgc gcgcggtttg ccgggaagaa agtttatgag attgccatct
    68221 cccaggcgtt gaaaaatatc gaccagattt tcagcgaatt accgacaaac tcgcagcttt
    68281 ttgccgcaga tagcggcgtg aagcttggca aacaggaagt gcaaagcggc agcgtgatga
    68341 gccacctgat ggcgggcgtc tctgcggcgc tgccgtttgt catcggcggt ggtatcctgg
    68401 tggcgcttgc caacatgctg gtgcagttcg gtttgccgta taccgatatg tcgaaaggcg
    68461 cgccgtcgtt tacctgggta gttgaatcca tcggttacct tggcttcacc tttatgatcc
    68521 ccatcatggg cgcttatatt gcctcgtcga ttgccgataa accggctttc gctccggcgt
    68581 ttctggtttg ctatctggcg aacgacaaag cgctgctcgg cacccagtcg ggtgcgggat
    68641 tcctcggcgc ggtggtgctg gggctggcga ttggctattt cgtcttctgg ttccgcaaag
    68701 tgcgccttgg caaagcatta caaccgctgc tcggttcaat gctgatcccg ttcgttactc
    68761 tgctggtttt cggcgtgctc acttactacg ttatcgggcc ggtgatgtcc gacctcatgg
    68821 gcgggctgct ccacttcctg aataccattc ctccatcaat gaagtttgcg gcagcgtttc
    68881 tggtcggtgc gatgctggcg ttcgatatgg gcggccccat caataaaacc gcctggttct
    68941 tctgcttctc actgctggaa aaacacattt atgactggta cgccatcgtc ggcgttgtcg
    69001 cgttgatgcc gcccgttgcg gcgggtcttg cgacgttcat tgcgcccaaa ctgtttaccc
    69061 gacaggaaaa agaggctgcc agtagtgcca ttgtggtcgg tgccacagtc gcaaccgaac
    69121 cggctatacc ttacgctctg gcagctccgt tgccgatgat taccgccaat acgctggcgg
    69181 gtggcattac cggcgtgctg gtgatcgctt tcggaatcaa acgtctggca cctggtttgg
    69241 gtatctttga cccgctgatc ggcctgatgt cgccggtggg ttcgttttat ctggtgctag
    69301 cgattggcct cgcgctgaac atttcattca ttatcgtcct gaaaggattg tggttacgcc
    69361 gcaaagcgaa agccgcgcaa caggagcttg tccatgaaca ttgagttact gcaacagttg
    69421 tgcgaagcca gcgccgtcag cggcgatgaa caggaagttc gcgacattct gataaacacg
    69481 ctggaacctt gcgttaatga gatcaccttt gatggtctgg gcagctttgt tgcccgtaag
    69541 gggaataaag gtccaaaagt tgccgttgtc gggcatatgg atgaagtcgg ctttatggtc
    69601 acccacatcg acgagagcgg ttttctgcgc tttaccacca ttggcggctg gtggaatcag
    69661 tcaatgctca accaccgggt aaccatacgc acacacaagg gatttaaaat ccctggtgtg
    69721 attggttccg tcgcacctca tgcgttaacg gaaaagcaaa agcaacagcc gctgtcattt
    69781 gatgagatgt tcattgatat tggcgcgaac agtcgcgatg aggtggaaaa acgcggtgtg
    69841 gcgatgggcg attttattag cccggaagcc aattttgcct gctggggcga agataaagtg
    69901 gtcggcaagg cgctggataa ccgcatcggc tgcgcaatga tggctgaact attgcagacg
    69961 gtgaataatc ccgaaattac gctgtatggc gtcggcagcg tggaagaaga agttgggcta
    70021 cgcggggcac aaacctcggc ggaacacatt aaaccggatg tggtgattgt gctggatacc
    70081 gctgtcgcag gtgatgttcc gggcattgat aacattaaat acccgctgaa actgggccag
    70141 gggccggggc tgatgctgtt tgacaagcgc tacttcccca accagaaact ggtggcggcg
    70201 ttaaaaagct gtgccgcaca taacgattta ccgctgcaat tttccaccat gaaaaccggt
    70261 gcgacggatg gcgggcgcta caacgtgatg ggcggcgggc gtccggttgt tgcgctgtgc
    70321 ctgccaaccc gctatctgca cgctaacagc ggcatgattt caaaagccga ttacgatgct
    70381 ctgctcacgt tgatacggga ttttctgacc accttaaccg cggagaaagt caacgcgttt
    70441 agccagttcc gtcaggtgga ttaatgctaa acgaacgcca gttaaagatt gtcgatctgc
    70501 tggagcaaca gccgcgcacg cctggtgagc tggcgcaaca gactggcgtt tcaggcagga
    70561 ccatcctgcg tgatattgac tatctcaact ttacccttaa cggcaaagcc cgcattttcg
    70621 ccagtggcag tgcgggctat cagctggaaa tcttcgagcg ccgcagcttt tttcagttgc
    70681 tgcaaaagca cgataacgac gatcggctgc tggcgctgtt attactgaat acctttactc
    70741 cccgtgcgca actcgcctcg gcgcttaatt tgccagaaac ctgggtagca gagcgtctgc
    70801 cccggttaaa acagcgttat gaacgcactt gttgcctggc cagtcgcccc ggtttgggcc
    70861 atttcattga tgagacagaa gagaaacgcg ttatcttgct ggcgaacttg ctgcgcaaag
    70921 atccgttttt aattccgctg gcgggcgtaa cacgagacaa ttttcagcat ttatccacgg
    70981 cctgcgacaa ccaacaccgc tggccgctca tgcagggtga ttatctctcc agcctgattc
    71041 tggcgattta cgccctgcgt aatcaactga ccgatgagtg gccgcaatat cccggtaacg
    71101 agataaaaca aatcgttgaa cagagcggca tgtttcttgg tgataacgct gtaagaaccc
    71161 tgacaggttt gatagagaaa cagcatcagc aagcgcaggt aatttcagcc gatcatgtgc
    71221 tggggttgct gcaaagggtg ccaggcatcg cgtcattgaa tattattgat acgcagctgg
    71281 ttgagaatat taccgggcat ttattacgtt gccttgccgc accggtgtgg attgccgagc
    71341 accgccaaag cagcatgaat aacctgaaag ccgcctggcc tgcggccttt gatatgagtc
    71401 tgcactttat tacgctactg cgtgaacagc tcgatattcc ccttttcgat agcgatctga
    71461 tcggtttgta ttttgcctgt gcgctggaac ggcatcaaaa cgaacgccag ccgatcattt
    71521 tgctctcgga ccagaacgcg attgccacta ttaatcagct cgccattgag cgcgatgttt
    71581 tacattgtcg ggtgattatt gcccgtagct taagcgagct tattgccatt cgcgaagaga
    71641 ttgagccgtt attgatcatt aacaacagcc attatttact ggatgacgcg gtaaataatt
    71701 acatcaccgt aaaaaatatc attacggctg ccgatatcga acaaataaaa cactttctgg
    71761 cgacggcatt tattcgccaa cagccggaac gctttttctc tgcccccgga agttttcatt
    71821 attcgaatgt acgcggtgaa agctggcaac atattacccg gcaaatttgt gcgcaattag
    71881 tggcacaaca ccatattacc gccgatgaag cgcaacggat catcgcccgc gaaggcgaag
    71941 gtgaaaacct gattgttaat cgcctcgcca tcccacattg ctggagcgaa caggagcgac
    72001 gttttcgtgg attttttatt accctcgccc aaccagttga ggtgaataac gaagtcatta
    72061 accatgtctt gatcgcctgc gccgccgccg atgcgcgtca tgagctaaaa atatttagct
    72121 atctggcaag cgtattgtgt cagcatccgg cagaagttat tgccgggtta acaggatatg
    72181 aggcatttat ggagttactt cacaaggggt gaactgtgtc ggatgcgccg catccgacac
    72241 tgcatttatc atcaaaaacg tttaacgcag gctattatag tcatcaacca tttcgttgta
    72301 ctcgcgtaac gctcgtggaa atgagtcttc gaccatccag cttgtgtttg catcctgaag
    72361 atgctcttta tcccagtcag agtacgggac tttatctctg acgcggcgaa catatttttt
    72421 agtctcgagt tgatactggt ctgccgaatt aatgaacgag aaattcatac cgcctttatc
    72481 cgcttctttg gcctgcgcta ccagcgtttc cagttcagag actttcttca ttgccgcatc
    72541 aacatcaaac ttctcttgcg aaatcaggtt attaatttgt ttcgctgaaa tcatgactgc
    72601 cagagagtag tagtggaatg ttttcccttc tctttcttca atatttttca gttccgcaag
    72661 ctggcgctta tcattaattt cctgaatcgc cgcatgataa gattctgcta caggttcaaa
    72721 ggattccaga ttttttaaga atgtctggtg cagcgttttc cctttcgcaa acgcatcgtc
    72781 tttataattt tcctgggtgt aatatttatc catttcgtta atggtattac ccaacgccac
    72841 ggcagcatca atataactca ccgccacgcc atcaataggt tgcagcgcag gcgttaacgc
    72901 cacagcactt tttacgcctt tttcacactc agcgaggttg gattcactaa tgccgtagat
    72961 gccataaaca gtactctctt taccggtagg cccctgttta aaatctttca gccagtcagc
    73021 ataacgcgcc agactgttct gtacagggat ctgtaacttg ttgtagcact tgatatagac
    73081 atccattttt tcgtcgaaaa ccgcttgttc atcgaccacc ggttgcgcag gtgtttcagg
    73141 tttagcttct gcttttgcaa cgggagcttc aggagctggt gcggcaggtt gactattggc
    73201 aggcgggggc gtttctgttt cggctttttt atcatcacaa cccgtcagac cgagggccat
    73261 gatggcgact ataatagcgg aagataataa attccttttc atttaattgc cttataaata
    73321 tatagataag agagtgaaca taataaatga aaaaagaaaa cgtcactaca cgcgttttat
    73381 ttataaataa aataataaaa ttcacgaaat gaataagtcc tgcaatttaa tatatatcgc
    73441 aggacttatt ttaattaatt gctcaaacgc tgcggatggg tataaaccgt tgcccgaccc
    73501 ggtttacaaa aacctaccag tgtcagatta cagcgctcag ccacttctac agcaagcgtg
    73561 gtcgcggcag acaccgcaaa caaaatctct acgccgcaca tcgccgactt ttgcaccatt
    73621 tcataactgg cacgactgga aaccagtacc gcaccttgct gccagctttc cccttcttgt
    73681 gaccggcggc ctaacagttt gtccagcgcc acatggcgac ccacgtcttc atgcccgccg
    73741 accagttcgc cagatggcaa catccaggca gcggcgtgag tacaaccagt cagttgcccc
    73801 actggctgaa aatcgttgag atgacgtaat gcatcatcca gtttgttgag atcaaacgtc
    73861 tgggtgaacg gtagcggctg caccggtttt ccgatgtcat taagttgctc cacgccgcat
    73921 acgccacagc ccgtacgtcc cgccagcgcc cggcggcgct ccttcaaccc cataaagcgg
    73981 cggctggaaa gctcaatttg tacttcaaga ccattacagg aaggaacgac atccatgccg
    74041 aagatatcgc gcggactttc tattatcccc tcggaaagcg aaaaaccgag cgcaaagtac
    74101 tcaaggtctt tgggcgacgc catcatcacc acatgcgaaa tgccgttgta gaccagcgca
    74161 acgggaactt cttccgcgac ctcatctaac cgtgggtgtt gtaaatcatc acgccgccat
    74221 aactcaattt gacgaacacc tgtgatattt gtcacatttt cgatttcttt tcgctgtgtt
    74281 tttttcactt tctcttaacc agataagaac acacacacca acattatggt attctgttac
    74341 aaacccttcc tggatggagg gaaattgagc caattctgga cctttgcggc cccttccgca
    74401 aagaaaaata actcccactc cctgcacacg cagcaagcga atgtaaatgg gacgtgacaa
    74461 tgtcgaaaca aggagcaatc catgcaggtc agcagaaggc agttctttaa gatctgcgct
    74521 ggcggtatgg caggcaccac ggcagcggca ctgggttttg cacccagcgt agcactcgcg
    74581 gaaacccggc agtataaact gctgcgcacc cgcgaaaccc gtaatacctg cacctattgt
    74641 tccgtaggct gtgggctgtt gatgtacagc ctcggtgacg gagcaaaaaa cgccaaagca
    74701 tctatcttcc atatcgaagg cgatccggat cacccggtca accgcggtgc actttgtccg
    74761 aaaggcgctg gcctggtgga tttcatccac tccgaaagcc gtctgaagtt tccggaatac
    74821 cgtgcgccag gttctgataa atggcagcaa atcagttggg aagaggcgtt tgatcgcatc
    74881 gccaaactga tgaaagaaga ccgcgatgct aactacattg cgcaaaacgc cgaaggcgtg
    74941 actgttaacc gctggctctc caccgggatg ctgtgtgctt ccgcgtcgag taacgaaacc
    75001 ggctatttaa cgcaaaaatt ctcccgcgcg ctgggtatgc tcgcggtcga caaccaggcg
    75061 cgtgtctgac acggaccaac ggtagcaagt cttgctccaa catttggtcg cggtgcgatg
    75121 accaaccact gggtcgacat caagaacgcc aacctcgtcg tggtgatggg cggtaacgcc
    75181 gctgaagctc acccggtcgg gttccgctgg gcgatggaag ccaaaattca caatggcgcg
    75241 aagctgattg tgatcgatcc tcgctttacg cgtacggcgg cggtggctga ctactatgcc
    75301 cctattcgtt ccggtactga cattgctttc ctgtcaggcg tattgctgta cctgctgaac
    75361 aatgaaaaat tcaaccgcga atacaccgaa gcctatacca acgccagcct gatcgtgcgt
    75421 gaggattacg gctttgaaga tggcctgttc accggctatg acgcggaaaa acgcaagtac
    75481 gataaatcct cctggactta cgaactggac gaaaacggct tcgccaaacg cgataccacg
    75541 ctgcaacatc cgcgctgcgt gtggaacttg ctgaaacagc acgtttcccg ctacacgcca
    75601 gatgtggttg aaaacatctg tggtacgcca aaagacgcgt tcctgaaagt ctgcgaatac
    75661 atcgcagaaa ccagtgctca cgataaaact gcctcgttcc tgtatgccct cggctggacg
    75721 caacactccg ttggtgcgca aaacattcgt acgatggcga tgatccagct gctgctcggc
    75781 aatatgggga tggcaggcgg cggcgttaac gccctgcgcg gtcactccaa tattcagggg
    75841 ctgacggacc tggggctgct gtcgcagagc ctgccaggtt acatgacgct gccaagcgag
    75901 aagcagaccg atctgcaaac ctaccttacc gccaacacgc caaaaccgct gctggaaggc
    75961 caggtaaact actggggcaa ctacccgaaa ttcttcgtct ctatgatgaa ggccttcttt
    76021 ggtgataaag cgacggcgga aaatagctgg ggctttgact ggttgccgaa gtgggataaa
    76081 ggctacgacg tcctgcaata cttcgagatg atgaaagagg gcaaggtcaa tggctatatc
    76141 tgccagggct ttaaccctgt tgcctcattc ccgaacaaaa acaaagtgat cggttgtctg
    76201 tcgaaactga agttcctcgt caccatcgac ccgctgaaca ctgaaacctc taacttctgg
    76261 cagaaccacg gtgagctgaa cgaagttgac tcgtcgaaga tccagaccga agtgttccgt
    76321 ctgccatcga cctgcttcgc agaagagaac ggttcaatcg ttaactcagg tcgctggttg
    76381 cagtggcact ggaaaggtgc ggacgcccca gggattgcgc tgactgatgg cgagatcctc
    76441 tccggtatct tcctgcgctt gcgcaagatg tatgccgaac agggtggcgc aaacccggat
    76501 caggtgctga acatgacctg gaactacgcc attccgcatg agccgaagtc ggaagaagtg
    76561 gcgatggaga gcaacggtaa ggcgctggcc gatattaccg atccggcaac cggtgcggtt
    76621 atcgtcaaga aaggccaaca acttagctcg ttcgcccaat tgcgcgatga cggtacaacc
    76681 tcctgtggct gctggatttt cgccggtagc tggacgccgg aaggcaacca gatggcgcgt
    76741 cgtgataacg ccgatccgtc tggcctcggt aacacgctgg gctgggcatg ggcatggccg
    76801 cttaaccgcc gcattctgta taaccgcgcc tccgcagatc cgcagggtaa cccgtgggat
    76861 ccgaagcgtc agttgctgaa atgggacggc actaagtgga ccggctggga tattccggac
    76921 tacagcgcag cgcctccggg cagcggcgtc gggccgttta tcatgcagca ggaaggcatg
    76981 gggcgtctgt ttgccctcga taagatggcg gaaggtccgt tcccggaaca ctacgagccg
    77041 tttgaaacgc cgctgggaac taacccgctg catccaaacg ttatctcgaa cccggctgcg
    77101 cgaatcttta aagacgacgc cgaagcattg ggtaaagccg ataagttccc gtatgtcgga
    77161 accacctatc gtctgaccga gcacttccac tactggacca aacacgcgct gttgaacgcg
    77221 attttgcaac cagagcagtt tgtggaaatc ggggagtcgc tggcgaataa acttggcatt
    77281 gcccagggcg ataccgtgaa agtctcctcc aaccgtggct atatcaaagc caaagcggtg
    77341 gtgaccaaac gtattcgcac gctgaaagcg aacggcaaag atatcgatac catcggtatt
    77401 ccgattcact ggggctatga aggcgtagct aaaaaaggct ttattgccaa tacgttaacg
    77461 ccattcgttg gtgatgcgaa cacgcagacg ccggagttta agtccttcct tgtgaatgtg
    77521 gaaaaggtgt aacggagacg acatatggct tatcaatcgc aagatatcat tcgtcgttcc
    77581 gcgactaacg gtctgacccc cgcgcctcag gcgcgggact tccaggaaga agtggcgaaa
    77641 ctcatcgacg tcaccacctg tatcggctgt aaagcctgtc aggtggcgtg ttcagagtgg
    77701 aacgacattc gcgataccgt cggcaataac attggggtgt acgacaaccc caatgattta
    77761 agcgccaaat cgtggacggt gatgcgcttc tcggaagtgg agcagaacga caaactggaa
    77821 tggctgatcc gcaaagacgg ctgtatgcac tgttccgatc caggctgcct gaaagcgtgc
    77881 ccggcggaag gtgcaattat tcagtatgcc aacggtatcg tcgacttcca gtccgagcag
    77941 tgcattggct gcggttattg cattgcgggc tgtccgttcg acattccgcg cctcaacccg
    78001 gaagacaacc gcgtctacaa atgtacgctg tgcgttgacc gcgtggtggt tgggcaagaa
    78061 ccagcctgcg tgaagacctg cccaacgggc gcgattcact tcggtacgaa agagtcgatg
    78121 aaaacgctgg cgagcgagcg cgttgctgag ctgaaaaccc gcggttacga caatgcgggt
    78181 ctgtacgatc cggcaggcgt cggtggtaca cacgtcatgt acgtgctgca ccatgctgac
    78241 aagccaaatc tgtatcatgg cttgccggag aacccggaaa tcagcgaaac cgtgaaattc
    78301 tggaaaggca tctggaaacc gctcgcggct gttggctttg cggctacctt tgcggccagt
    78361 atcttccact acgtgggtgt cggtccgaac cgtgcggatg aggaagagaa taatctgcac
    78421 gaagagaaag acgaggagcg caaatgaaac gacgtgacac catcgtgcgc tacaccgcgc
    78481 cggaacgtat caaccactgg atcaccgcct tctgcttcat cctggcggcg gtgagcgggc
    78541 tgggcttttt gttcccgtcc ttcaactggt tgatgcaaat catgggcaca ccgcagctgg
    78601 cgcgaattct gcacccgttt gtcggcgtgg ttatgtttgc ctcgttcatc atcatgtttt
    78661 tccgttactg gcatcacaac ctaatcaatc gggatgatat cttttgggcg aagaatattc
    78721 gtaagatcgt cgtcaacgag gaagtaggtg acaccgggcg ttataacttc ggtcagaaat
    78781 gcgttttctg ggcggcgatt attttcctgg ttctgctgct ggtgagcggc gtgattatct
    78841 ggcgtcctta ttttgcgcct gctttctcaa tcccggtgat ccgattcgcg ttaatgctgc
    78901 attcatttgc cgcagtggcg ttaattgtgg ttatcatggt gcatatctac gccgcccttt
    78961 gggtgaaagg cacgattacc gccatggtgg aaggatgggt taccagcgcc tgggcgaaga
    79021 aacatcaccc gcgctggtac cgtgaggtcc gcaagacaac ggaaaagaaa gctgaatgag
    79081 tattcgcata atcccgcaag atgagctggg ttcgagcgag aaacgtacgg cggatatgat
    79141 tccgccgtta ttgttccctc ggctcaagaa tttatacaac cgccgcgccg agcgtctgcg
    79201 cgagctggca gaaaataatc cgctgggtga ttacctgcgc tttgctgcgc ttatcgccca
    79261 cgcccaggaa gtggtgctgt acgaccatcc gctggagatg gatctgaccg cgcgcattaa
    79321 agaagccagc gcacagggca agcccccgct ggatattcac gttctgccgc gtgataagca
    79381 ctggcaaaag ctgctgatgg cgctaattgc tgagctgaaa cctgaaatga gcggcccggc
    79441 gctggcagtg attgagaatc tggagaaggc atcgactcag gagctggaag atatggccag
    79501 cgcgctgttt gcctctgatt tctcgtccgt cagcagcgat aaagcgccgt ttatctgggc
    79561 tgcgctgtcg ctctactggg cgcagatggc caatctgatc cccggcaaag cccgcgctga
    79621 atacggcgaa caacgtcaat attgcccggt atgtggctct atgccggtgt ccagcatggt
    79681 gcaaattggc accactcagg gtctgcgtta cctgcactgc aacctgtgtg aaaccgaatg
    79741 gcacgtagtg cgcgtaaaat gcagcaactg cgagcagagc ggcaaactgc attactggtc
    79801 gctggatgac gaacaggccg cgattaaagc cgaaagctgc gatgactgcg gcacttacct
    79861 gaagattctt tatcaggaaa aagaaccgaa agttgaagcc gtggcagatg acctcgcctc
    79921 tctggtactg gacgcgcgaa tggagcaaga aggctacgcc cgtagttcca ttaacccgtt
    79981 cctgtttccg ggtgaaggag agtaatctct gacgttttcc gggtggcgca ggtcgcccgg
    80041 attacagctc atagggtgtg acgacaccat ctctcgtatt ccgcgttacc cattcaagat
    80101 catgccgcaa ttgtctgttt gcctcgttct tccctccaac acatcgataa ggttatgggt
    80161 atgtcactga tgacaagcgt atgattgcct gatgcgctgc gcttatcagg cctaccaata
    80221 aacttcaata tattgaattt gcatgacttt gtaggccgga taaggcgttc acgccgcatc
    80281 cggcatgaac aacgcgcacg ttgtcagcaa tctgattgac atcatttttt cccattacca
    80341 ttcctcgcgc agcttacgct gatattcgac accatcttcc tcacaccctt gccagatgcc
    80401 aggaacactg gttcttgctg tttgcgattg atttatcagg tattgatcta ccgcttccct
    80461 taataggtcc gcacgaggaa gattacgctg cacctcgaga tcatcaagtt gcttaatcac
    80521 ctcattcgat aaatcgagta aaattctgcc catatctata cctgccagag agttcataaa
    80581 tatctcgcca atatcatttt tgaatctatg gagagaaaag tacccttgcc gaatctttaa
    80641 agaaagctca tttacgcatc attttttatt tttgcgaggc gttttccaga aataataaga
    80701 gcgttgcagt tgtttcattt attactcaaa aatggtttag ttatcgccca ggatgagtta
    80761 tgggtgctat gaatatatta tattcaggga tatttatttg cataatctga atggtgtagc
    80821 cgaaaaacgt cagcatccta acagcaccag gatttaaggt gaaattaatc tttcatcaca
    80881 gaccggtcag tactgccacc aaccggtctt tacttgctca tcgttataat tttgttggta
    80941 acagttcggg aaagttgttt ttctaaatta acaacagaat aactttcttg tttaatgaga
    81001 ttaatattgg caaccattat agcctccttc ccacggttta agagcaaaca tacaacataa
    81061 tgattcataa catacagagc agtaaatgaa tgatactgac aatgatgcca gcccattatc
    81121 tttgcataat gtttgtgagc tatagatttt aaaaatattt atgagaaaag tatggcacca
    81181 gaggaggttt tgactatcaa aaatcgctca aacctgaact aagatgatga gttaagcatt
    81241 atctgctata attgccactc agctctacaa ataatattta aatgctcctg aacagtgatg
    81301 tagcaagaca cgtatggtgc caacgaagaa taaaaattta tagagttctt attcaatatt
    81361 accggcatag acagggaatg atgtaaat
//
LOCUS       DAESHI010000003        79579 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.003, whole genome shotgun sequence.
ACCESSION   DAESHI010000003 DAESHI010000000
VERSION     DAESHI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 79579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79579
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.003"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            2..1197
                     /gene="bluF"
                     /locus_tag="KW011_000178"
                     /pseudo
     CDS             2..1197
                     /gene="bluF"
                     /locus_tag="KW011_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
     gene            1402..2133
                     /gene="bluR"
                     /locus_tag="KW011_000179"
     CDS             1402..2133
                     /gene="bluR"
                     /locus_tag="KW011_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HBH7658624.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2354..2758
                     /locus_tag="KW011_000180"
     CDS             2354..2758
                     /locus_tag="KW011_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HBH7658625.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            2811..2921
                     /locus_tag="KW011_000181"
     CDS             2811..2921
                     /locus_tag="KW011_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7658626.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQANTIKLIFEM"
     gene            3157..3201
                     /locus_tag="KW011_000182"
     CDS             3157..3201
                     /locus_tag="KW011_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7658627.1"
                     /translation="MFSKLAPSSIKAMF"
     gene            3454..3777
                     /gene="iraM"
                     /locus_tag="KW011_000183"
     CDS             3454..3777
                     /gene="iraM"
                     /locus_tag="KW011_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HBH7658628.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGSIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(3880..5130)
                     /gene="icd"
                     /locus_tag="KW011_000184"
     CDS             complement(3880..5130)
                     /gene="icd"
                     /locus_tag="KW011_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HBH7658629.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            5302..5955
                     /gene="rluE"
                     /locus_tag="KW011_000185"
     CDS             5302..5955
                     /gene="rluE"
                     /locus_tag="KW011_000185"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HBH7658630.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            5965..6426
                     /gene="nudJ"
                     /locus_tag="KW011_000186"
     CDS             5965..6426
                     /gene="nudJ"
                     /locus_tag="KW011_000186"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HBH7658631.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            6480..7586
                     /gene="mnmA"
                     /locus_tag="KW011_000187"
     CDS             6480..7586
                     /gene="mnmA"
                     /locus_tag="KW011_000187"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBH7658632.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            7622..8263
                     /gene="hflD"
                     /locus_tag="KW011_000188"
     CDS             7622..8263
                     /gene="hflD"
                     /locus_tag="KW011_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HBH7658633.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            8267..9637
                     /gene="purB"
                     /locus_tag="KW011_000189"
     CDS             8267..9637
                     /gene="purB"
                     /locus_tag="KW011_000189"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBH7658634.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            9805..10476
                     /gene="phoP"
                     /locus_tag="KW011_000190"
     CDS             9805..10476
                     /gene="phoP"
                     /locus_tag="KW011_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HBH7658635.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            10476..11936
                     /gene="phoQ"
                     /locus_tag="KW011_000191"
     CDS             10476..11936
                     /gene="phoQ"
                     /locus_tag="KW011_000191"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HBH7658636.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            12012..13133
                     /gene="roxA"
                     /locus_tag="KW011_000192"
                     /gene_synonym="ycfD"
     CDS             12012..13133
                     /gene="roxA"
                     /locus_tag="KW011_000192"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HBH7658637.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(13182..14408)
                     /gene="pepT"
                     /locus_tag="KW011_000193"
     CDS             complement(13182..14408)
                     /gene="pepT"
                     /locus_tag="KW011_000193"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBH7658638.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            14658..15794
                     /gene="potA"
                     /locus_tag="KW011_000194"
     CDS             14658..15794
                     /gene="potA"
                     /locus_tag="KW011_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HBH7658639.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            15778..16635
                     /gene="potB"
                     /locus_tag="KW011_000195"
     CDS             15778..16635
                     /gene="potB"
                     /locus_tag="KW011_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HBH7658640.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            16632..17426
                     /gene="potC"
                     /locus_tag="KW011_000196"
     CDS             16632..17426
                     /gene="potC"
                     /locus_tag="KW011_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HBH7658641.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            17423..18469
                     /gene="potD"
                     /locus_tag="KW011_000197"
     CDS             17423..18469
                     /gene="potD"
                     /locus_tag="KW011_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HBH7658642.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(18625..19446)
                     /gene="cobB"
                     /locus_tag="KW011_000198"
     CDS             complement(18625..19446)
                     /gene="cobB"
                     /locus_tag="KW011_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBH7658643.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(19462..20373)
                     /gene="nagK"
                     /locus_tag="KW011_000199"
     CDS             complement(19462..20373)
                     /gene="nagK"
                     /locus_tag="KW011_000199"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBH7658644.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(20402..21646)
                     /gene="lolE"
                     /locus_tag="KW011_000200"
     CDS             complement(20402..21646)
                     /gene="lolE"
                     /locus_tag="KW011_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HBH7658645.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(21646..22347)
                     /gene="lolD"
                     /locus_tag="KW011_000201"
     CDS             complement(21646..22347)
                     /gene="lolD"
                     /locus_tag="KW011_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HBH7658646.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(22340..23539)
                     /gene="lolC"
                     /locus_tag="KW011_000202"
     CDS             complement(22340..23539)
                     /gene="lolC"
                     /locus_tag="KW011_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HBH7658647.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            23801..24874
                     /locus_tag="KW011_000203"
     CDS             23801..24874
                     /locus_tag="KW011_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBH7658648.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            25002..28448
                     /gene="mfd"
                     /locus_tag="KW011_000204"
     CDS             25002..28448
                     /gene="mfd"
                     /locus_tag="KW011_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBH7658649.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            28592..29548
                     /gene="ldtC"
                     /locus_tag="KW011_000205"
                     /pseudo
     CDS             28592..29548
                     /gene="ldtC"
                     /locus_tag="KW011_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(29631..29888)
                     /gene="bhsA"
                     /locus_tag="KW011_000206"
     CDS             complement(29631..29888)
                     /gene="bhsA"
                     /locus_tag="KW011_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HBH7658650.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            30129..30761
                     /gene="comR"
                     /locus_tag="KW011_000207"
     CDS             30129..30761
                     /gene="comR"
                     /locus_tag="KW011_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HBH7658651.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(30823..31362)
                     /locus_tag="KW011_000208"
     CDS             complement(30823..31362)
                     /locus_tag="KW011_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBH7658652.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(31589..32893)
                     /gene="ndh"
                     /locus_tag="KW011_000209"
     CDS             complement(31589..32893)
                     /gene="ndh"
                     /locus_tag="KW011_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HBH7658653.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(33301..33843)
                     /locus_tag="KW011_000210"
     CDS             complement(33301..33843)
                     /locus_tag="KW011_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH7658654.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(33866..34891)
                     /gene="nagZ"
                     /locus_tag="KW011_000211"
     CDS             complement(33866..34891)
                     /gene="nagZ"
                     /locus_tag="KW011_000211"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HBH7658655.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(34902..35726)
                     /gene="thiK"
                     /locus_tag="KW011_000212"
     CDS             complement(34902..35726)
                     /gene="thiK"
                     /locus_tag="KW011_000212"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HBH7658656.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(35707..36348)
                     /gene="lpoB"
                     /locus_tag="KW011_000213"
     CDS             complement(35707..36348)
                     /gene="lpoB"
                     /locus_tag="KW011_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HBH7658657.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(36362..36739)
                     /locus_tag="KW011_000214"
     CDS             complement(36362..36739)
                     /locus_tag="KW011_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HBH7658658.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(36742..37101)
                     /gene="hinT"
                     /locus_tag="KW011_000215"
     CDS             complement(36742..37101)
                     /gene="hinT"
                     /locus_tag="KW011_000215"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HBH7658659.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            37435..39624
                     /gene="fhuE"
                     /locus_tag="KW011_000216"
     CDS             37435..39624
                     /gene="fhuE"
                     /locus_tag="KW011_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HBH7658660.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQLDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLVFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(39684..41117)
                     /gene="ptsG"
                     /locus_tag="KW011_000217"
     CDS             complement(39684..41117)
                     /gene="ptsG"
                     /locus_tag="KW011_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HBH7658661.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(41412..42209)
                     /locus_tag="KW011_000218"
     CDS             complement(41412..42209)
                     /locus_tag="KW011_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBH7658662.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(42220..43224)
                     /gene="holB"
                     /locus_tag="KW011_000219"
     CDS             complement(42220..43224)
                     /gene="holB"
                     /locus_tag="KW011_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBH7658663.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(43221..43862)
                     /locus_tag="KW011_000220"
     CDS             complement(43221..43862)
                     /locus_tag="KW011_000220"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBH7658664.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(43852..44874)
                     /gene="yceG"
                     /locus_tag="KW011_000221"
     CDS             complement(43852..44874)
                     /gene="yceG"
                     /locus_tag="KW011_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HBH7658665.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(44877..45686)
                     /gene="pabC"
                     /locus_tag="KW011_000222"
     CDS             complement(44877..45686)
                     /gene="pabC"
                     /locus_tag="KW011_000222"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HBH7658666.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(45806..47047)
                     /gene="fabF"
                     /locus_tag="KW011_000223"
     CDS             complement(45806..47047)
                     /gene="fabF"
                     /locus_tag="KW011_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBH7658667.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(47135..47371)
                     /gene="acpP"
                     /locus_tag="KW011_000224"
     CDS             complement(47135..47371)
                     /gene="acpP"
                     /locus_tag="KW011_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBH7658668.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(47582..48316)
                     /gene="fabG"
                     /locus_tag="KW011_000225"
     CDS             complement(47582..48316)
                     /gene="fabG"
                     /locus_tag="KW011_000225"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HBH7658669.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(48329..49258)
                     /gene="fabD"
                     /locus_tag="KW011_000226"
     CDS             complement(48329..49258)
                     /gene="fabD"
                     /locus_tag="KW011_000226"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBH7658670.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(49274..50227)
                     /gene="fabH"
                     /locus_tag="KW011_000227"
     CDS             complement(49274..50227)
                     /gene="fabH"
                     /locus_tag="KW011_000227"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HBH7658671.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(50295..51365)
                     /gene="plsX"
                     /locus_tag="KW011_000228"
     CDS             complement(50295..51365)
                     /gene="plsX"
                     /locus_tag="KW011_000228"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBH7658672.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(51446..51619)
                     /gene="rpmF"
                     /locus_tag="KW011_000229"
     CDS             complement(51446..51619)
                     /gene="rpmF"
                     /locus_tag="KW011_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBH7658673.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(51671..52192)
                     /gene="yceD"
                     /locus_tag="KW011_000230"
     CDS             complement(51671..52192)
                     /gene="yceD"
                     /locus_tag="KW011_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HBH7658674.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            52391..52975
                     /gene="yceF"
                     /locus_tag="KW011_000231"
     CDS             52391..52975
                     /gene="yceF"
                     /locus_tag="KW011_000231"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HBH7658675.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENACLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(53087..54046)
                     /gene="rluC"
                     /locus_tag="KW011_000232"
     CDS             complement(53087..54046)
                     /gene="rluC"
                     /locus_tag="KW011_000232"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HBH7658676.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            54619..57804
                     /gene="rne"
                     /locus_tag="KW011_000233"
     CDS             54619..57804
                     /gene="rne"
                     /locus_tag="KW011_000233"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HBH7658677.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(57999..58952)
                     /gene="flgL"
                     /locus_tag="KW011_000234"
     CDS             complement(57999..58952)
                     /gene="flgL"
                     /locus_tag="KW011_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBH7658678.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(58964..60607)
                     /gene="flgK"
                     /locus_tag="KW011_000235"
     CDS             complement(58964..60607)
                     /gene="flgK"
                     /locus_tag="KW011_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBH7658679.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(60673..61614)
                     /gene="flgJ"
                     /locus_tag="KW011_000236"
     CDS             complement(60673..61614)
                     /gene="flgJ"
                     /locus_tag="KW011_000236"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HBH7658680.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(61614..62711)
                     /gene="flgI"
                     /locus_tag="KW011_000237"
     CDS             complement(61614..62711)
                     /gene="flgI"
                     /locus_tag="KW011_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HBH7658681.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(62723..63421)
                     /gene="flgH"
                     /locus_tag="KW011_000238"
     CDS             complement(62723..63421)
                     /gene="flgH"
                     /locus_tag="KW011_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HBH7658682.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(63474..64246)
                     /gene="flgG"
                     /locus_tag="KW011_000239"
                     /pseudo
     CDS             complement(63474..64246)
                     /gene="flgG"
                     /locus_tag="KW011_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(64418..65173)
                     /gene="flgF"
                     /locus_tag="KW011_000240"
     CDS             complement(64418..65173)
                     /gene="flgF"
                     /locus_tag="KW011_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HBH7658683.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(65193..66398)
                     /gene="flgE"
                     /locus_tag="KW011_000241"
     CDS             complement(65193..66398)
                     /gene="flgE"
                     /locus_tag="KW011_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HBH7658684.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(66423..67118)
                     /gene="flgD"
                     /locus_tag="KW011_000242"
     CDS             complement(66423..67118)
                     /gene="flgD"
                     /locus_tag="KW011_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HBH7658685.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(67130..67534)
                     /gene="flgC"
                     /locus_tag="KW011_000243"
     CDS             complement(67130..67534)
                     /gene="flgC"
                     /locus_tag="KW011_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBH7658686.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNMVKSMMLKTLTLGQ"
     gene            complement(67538..67954)
                     /gene="flgB"
                     /locus_tag="KW011_000244"
     CDS             complement(67538..67954)
                     /gene="flgB"
                     /locus_tag="KW011_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBH7658687.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            68109..68768
                     /gene="flgA"
                     /locus_tag="KW011_000245"
     CDS             68109..68768
                     /gene="flgA"
                     /locus_tag="KW011_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HBH7658688.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGNGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            68844..69137
                     /gene="flgM"
                     /locus_tag="KW011_000246"
     CDS             68844..69137
                     /gene="flgM"
                     /locus_tag="KW011_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HBH7658689.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            69142..69558
                     /gene="flgN"
                     /locus_tag="KW011_000247"
     CDS             69142..69558
                     /gene="flgN"
                     /locus_tag="KW011_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HBH7658690.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(69598..71133)
                     /gene="murJ"
                     /locus_tag="KW011_000248"
     CDS             complement(69598..71133)
                     /gene="murJ"
                     /locus_tag="KW011_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBH7658691.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(71243..72166)
                     /locus_tag="KW011_000249"
     CDS             complement(71243..72166)
                     /locus_tag="KW011_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBH7658692.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(72168..72815)
                     /locus_tag="KW011_000250"
     CDS             complement(72168..72815)
                     /locus_tag="KW011_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HBH7658693.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(72826..73410)
                     /gene="rimJ"
                     /locus_tag="KW011_000251"
     CDS             complement(72826..73410)
                     /gene="rimJ"
                     /locus_tag="KW011_000251"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HBH7658694.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            73646..74854
                     /gene="mdtH"
                     /locus_tag="KW011_000252"
     CDS             73646..74854
                     /gene="mdtH"
                     /locus_tag="KW011_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HBH7658695.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            74918..75565
                     /gene="grxB"
                     /locus_tag="KW011_000253"
     CDS             74918..75565
                     /gene="grxB"
                     /locus_tag="KW011_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HBH7658696.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            75699..76259
                     /locus_tag="KW011_000254"
     CDS             75699..76259
                     /locus_tag="KW011_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBH7658697.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            76365..77411
                     /gene="pyrC"
                     /locus_tag="KW011_000255"
     CDS             76365..77411
                     /gene="pyrC"
                     /locus_tag="KW011_000255"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBH7658698.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLITCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            77485..77730
                     /gene="dinI"
                     /locus_tag="KW011_000256"
     CDS             77485..77730
                     /gene="dinI"
                     /locus_tag="KW011_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HBH7658699.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            78020..78274
                     /gene="bssS"
                     /locus_tag="KW011_000257"
     CDS             78020..78274
                     /gene="bssS"
                     /locus_tag="KW011_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HBH7658700.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            78389..79507
                     /gene="solA"
                     /locus_tag="KW011_000258"
     CDS             78389..79507
                     /gene="solA"
                     /locus_tag="KW011_000258"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HBH7658701.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLNAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPEQPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT        19594 a        20913 c        19715 g        19357 t
ORIGIN      
        1 aatgcttacc acccttattt atcgtagcca tatacgtgac gacgaacctg tcaaaaaaat
       61 cgaagaaatg gtttcgatag caaatcgcag gaacatgcag tctgacgtaa cagggatctt
      121 actgtttaat ggttctcatt ttttccagct tctggaaggt ccggaagaac aggttaaaat
      181 gatatatcgg gctatatgcc aggatccacg gcactataat attgttgagc tgctgtgcga
      241 ttacgcgcct gctcgccgtt ttggcaaagc gggaatggaa ttatttgatt tgcgcctgca
      301 cgagcgagat gacgttttac aggccgtatt cgacaaaggc acatcaaaat ttcagctaac
      361 ttatgatgac agagcgctac aattttttcg tacttttgtc cttgcaaccg aacaatcaac
      421 ctatttcgag atccctgccg aagactcctg gctttttatc gctgacggat ctgataaaga
      481 acttgattcc tgtacccttt caccaactat aaacgaccac tttgcctttc atcccttatc
      541 gcggcggata atcgcttttg aagccattgt gcaaaaaaat gaagcccatc agccatagcg
      601 gttgggcagc gtaaagacgg ggaaatctac acagcggatc tcaaaagtaa ggcgcttgca
      661 ttcgcgatgg cacacgcact tgagctcggt gataaaatga tttcaatcaa tctattacct
      721 atgaccctgg ttaacgaacc tgacgcagtc tcttttttac ttgatgaaat aaaggccaat
      781 gctctggtgc ctgaacaaat catcgttgaa tttactgaaa gtgaagttat atctcggttt
      841 gatgagtttg cagaagcgat taaatcgctt aaggctgccg gtatcagtgt agcaattgat
      901 cattttggcg caggttttgc cggtttgtta ctcctgtcac gcttccagcc tgacagaatt
      961 aaaatcagtc aggaattgat taccaatgtt cataaaagcg ggccacggca ggcaattatt
     1021 caagcgatca taaaatgctg tacatcactt gaaattcaag tcagtgctat gggcgtggca
     1081 acaccagaag agtggatgtg gcttgaatcc gcaggaattg agatgtttca gggagatctg
     1141 tttgctaaag ctaaattgaa tggtatccct tccatagcgt ggccagagaa aaaataattt
     1201 tcagcacatt ctttcacatg atttcagtaa atcatcgtga aaatgaatac gttatgttta
     1261 aatttaatcc acattaaata taatgtttca tccaatttag ctgaaatctt atttatactt
     1321 tgtacaattt tagcgtatat tgtacaaagt attattgggt cgtgtacagg cggcggagat
     1381 ttatgaccgc aaggaggaat tgtggcttat tacagcattg gtgatgttgc tgaacgttgc
     1441 gggattaatc ctgtcactct ccgggcctgg caacgccgct acggtttgtt aaaaccacag
     1501 cgcagtgaag gcggacaccg actctttgat gaagaagaca tacaacgcat cgaagagatc
     1561 aagcgttgga taagtaatgg cgtccctgta ggcaaagtta aagcattact ggaaaccacc
     1621 agccaggata cggaagatga ctggaaccgc ctgcaagaag agatgatgtc aattcttcgt
     1681 atggctaatc ctgccaaact acgcgcgaga attatttcac tgggtcgaga gtacccagta
     1741 gatcaattga ttaatcatgt ttaccttcct gttcgccagc gtctcgtgct tgatcacaac
     1801 acctcccgca ttatgagcag tatgtttgac ggcgcattaa ttgaatatgc agcagcctca
     1861 cttttcgaaa tgcgccgtaa gcccggtaaa gaagccattc tgatggcgtg gaatgttgaa
     1921 gagagagcac gattgtggct ggaagcatgg cgtttatcat tgtcaggatg gcacatttct
     1981 gttcttgctg atcccattga atcgccgcgc ccggaactgt tcccgacgca aacattgatt
     2041 gtctggacag gcatggcacc aacgagaagg caaaacgaac ttttacaaca ttggggtgag
     2101 caaggttata aagtcatctt tcatgccccc taatttcacc tttatcccgc cccttagaca
     2161 gaatgttata atctgaaccc acaacagcat ttgtaccgga gctggtccgt tgcaaaatgc
     2221 tcaccggaag aattcaggta atccctctat cacactctgt acatatcaca gccaaaggac
     2281 agaccagatt gaagtggtaa aaccgatcgt gcacactacg cagtctcaca actaacaaga
     2341 ggttattatc ctgatggatc aggtcgttgt ttttcaaaag atgtttgagc aagtgagaaa
     2401 agagcaaaac ttctcctggt tttattcaga attaaaacat caccgtattg cacattacat
     2461 ttattatctg gctacggata acatcagaat tattactcac gatgacacgg ttttgttatt
     2521 aagaggaacc aggaacctgt taaaagttag tacgaccaag aaccctgcta aaataaaaga
     2581 ggccgcattg cttcatattt acggaaaatc tacatttcgg gaatactgtt caacactggc
     2641 aggcgcgggc gttttccgtt gggttactga tgttaatcat aacaaacgca gttactacgc
     2701 cattgataat acgcttttat atattgaaga tgtagaaaat aataaaccat taatctagct
     2761 aaagttggat gcttaagaaa tgcttcataa ttcagtaagg cattagcata atgggaataa
     2821 aagtacagag actatcccta tggatgataa atactgtcct tttattgcta cccataaata
     2881 atcaccaggc caatactatc aaattgatat ttgaaatgtg atcacttgac tttctatacg
     2941 ttattttata acggttaata tatttataaa aacaacgggt gtgccatacg cccgtttcaa
     3001 tacttaacgc acatgtgttt tggtttagtc atcatccagt tatatgtatt ttagccagga
     3061 acaggttaaa cctttcctat ataactcaaa aattgaaacc ttattctcat gtcatgttta
     3121 tattcattat tatcgttata taaaaaggca accataatgt ttagcaaatt ggcaccaagt
     3181 agcataaagg ctatgtttta attacaggat gttcagtcat ttgaatgtat aacattatag
     3241 ctaaacaaat ctaaaacgaa gtgaataatt tattgttttc actaaatctc attttgttta
     3301 acatccattg agattccttg ctttaaattt tgttttatat aagcaatcgt tttaattaat
     3361 ttattttttg aaggggtaat atactcatat gcaaaataaa gaaatgaaca tccaaatgaa
     3421 ctatattaaa taccgtggga taagacataa caaatgaagt gggtagtaat tgacacggta
     3481 attcaaccta catgcggcat atctttttca gccatatggg gtgatatgaa aatgatcatc
     3541 tggtatcaat ctactatatt tcttcctcct ggaagtatat ttacaccggt taagcttggt
     3601 attatcctta aggaaaaaga atatcctatt actatttata acattgcacc attcaacaag
     3661 aatttatgga gtttactcaa aagcagtcaa gagtgtcctc caggagaaag caaaataaca
     3721 aataaatgtt tacataatag ttgcattata aaaatatgcc catatgggct caagtaatgg
     3781 gcttcatata aatatgctcg atatgctatt aataatatcc acataaataa aacaacgggc
     3841 gtgttatacg cccgtttcaa tatttaactc atggagagat tacatgtttt cgatgatcgc
     3901 ttcaccaaac tctgaacatt tcagcagctt agcgccttcc atcagacgtt caaagtcata
     3961 agttacggtt ttcgcgttga tagcaccttc cataccttta acaattaaat cagccgcttc
     4021 agtccacccc atatggcgca gcatcatctc agcggagagg atgatagagc ctggatttac
     4081 tttgtcctga ccggcatatt tcggcgcagt accgtgggtg gcttcaaaca gggcgcattc
     4141 gtcaccgatg tttgcgccag gggcgatacc gataccgcca acctgcgctg ccagggcgtc
     4201 agaaatgtag tcaccgttca ggttcataca ggcgataaca tcatattcag ccggacgcag
     4261 caggatctgt tgcaggaatg catcagcaat cacgtcttta atgacgatct ctttgccggt
     4321 gttcgggttt ttaactttca gccacgggcc gccgtcgatc agttcaccgc caaactcttc
     4381 acgcgccagc tggtagcccc agtctttaaa cgcgccttcg gtgaacttca tgatgttgcc
     4441 tttgtgcacc agagtcacag agtcacgatc gttagcaatt gcgtattcga tcgctgcacg
     4501 aaccagacgt ttggtgcctt cttccgaaca cggcttaata ccgataccac aatgttccgg
     4561 gaagcgaatt ttcttcaccc ccatctcttc acgcaggaat ttaatcactt tctcggcgtc
     4621 ggcagagtct gctttccatt cgatacccgc ataaatgtct tccgagtttt cacggaagat
     4681 aaccatatcg gtcagttcag ggtgtttaac cgggcttgga gtgccctgat agtaacgtac
     4741 cggacgcagg cagatgtaga gatccagttc ctggcgcagg gcaacgttca gagagcgaat
     4801 accgccacca acaggagtgg tcagcggacc tttaatggca acgcgatatt cacgaatcag
     4861 atcaagggtt tcagcaggca gccagacatc ctgaccataa acctgtgtgg atttttcacc
     4921 ggtgtaaatt tccatccagg agattttacg ctcgccttta taggctttct cgactgcagc
     4981 gtcgaccact ttcagcatgg ctggggttac atctacaccg attccatcac cttcaatgta
     5041 agggataatc ggattttcag gaacgttgag tttgccgttt tgcagggtga tcttcttgcc
     5101 ttgtgccgga acaactactt tactttccat tcacctctcc ttcgagcgct actggtttgc
     5161 tcgtctgcca ccacgttgca tatgcgtttg cgtcctgcga tacggatgct ttagagcaat
     5221 tttttgttaa tgatttgtaa ttggcttgtc aatgctaccc tattgtttgc cgtcatgaaa
     5281 gatgcgcgtt attaggctat aatgcggcaa ttcataatct ctgaaaatac catgcaaaaa
     5341 acttctttta gaaatcacca ggttaagcga ttcagctcgc aacgttctac taggcgtaaa
     5401 cctgaaaacc agcccacgcg tgtgatcctg ttcaataaac cctacgatgt tcttccgcag
     5461 ttcaccgatg aagccggacg caaaacatta aaagaattca tcccggttca gggcgtttat
     5521 gcagcaggtc gtcttgaccg cgatagcgaa gggttactgg tgctgaccaa taacggcgcg
     5581 ttgcaggcgc gtttaaccca gccgggcaaa cgcaccggga aaatctatta tgtgcaggtg
     5641 gaaggtattc ccacacaaga cgcacttgaa gccttgcgca atggcgtaac cttaaatgat
     5701 ggccctaccc tgcccgccgg cgcggagctg gttgacgaac cagcgtggtt atggccgcgg
     5761 aatccaccaa ttcgtgaacg caaaagtatt cccaccagct ggctgaagat caccttatat
     5821 gaaggacgta atcgccaggt gcgccgcatg accgcccatg ttggtttccc cacgctgcga
     5881 ctgattcgtt atgcgatggg tgattacact ttggataacc ttgccaatgg cgaatggcga
     5941 gaagcaacag aataaggatg cacaatgttt aaaccgcacg ttaccgttgc ttgcgtagtg
     6001 cacgcagagg gtaaattttt agtcgttgaa gagacgatta atggtaaagc gttatggaac
     6061 caacctgccg ggcatctgga agccgatgaa accttagtgg aagctgccgc ccgtgagcta
     6121 tgggaagaaa ccggcatcag cgcgcaaccg caacacttta ttcgtatgca tcagtggatt
     6181 gcgccagata aaacgccgtt tttgcgtttc ctctttgcca ttgagcttga gcaaatatgc
     6241 ccgacccagc ctcatgacag cgatatcgac tgctgtcgtt gggtcagcgc cgaagaaatt
     6301 ttaaaggcgt caaatcttcg ttcgccgctg gtggcggaaa gtatccgttg ttatcaaagt
     6361 gggcaacgct atccgctgga gatgattggc gattttaact ggccttttac aaagggtgtc
     6421 atctaaaagg ctgcgtgata gaatacgccg ccttgaagtt caatgtcgtg agtgatccaa
     6481 tgtctgaaac cgcaaaaaaa gtaatcgtcg gcatgtccgg cggtgtcgat tcctccgttt
     6541 ctgcctggct gttgcaacaa cagggatatc aggttgaagg cctgtttatg aagaactggg
     6601 aagaagacga cggtgaggaa tattgcacag cggcagcgga tctggctgat gcccaggctg
     6661 tctgcgacaa gctcggcatt gaactgcaca ccgttaactt tgctgccgag tactgggaca
     6721 atgtcttcga actgttcctt gccgaatata aagccggtcg cacgccgaat ccggatattc
     6781 tgtgcaacaa agagatcaaa tttaaagctt tcctcgaatt tgccgccgaa gatttaggtg
     6841 ccgattatat cgctaccggt cattacgtac gtcgggccga tgtcgatggc aagagccgcc
     6901 tgctgcgtgg tctggacagc aataaagacc agagctactt cctttatacg ctcagccatg
     6961 agcagattgc gcaaagcctg ttcccggtcg gcgaactgga aaaaccgcag gtgcgtaaga
     7021 ttgctgaaga tcttggtctg gtcaccgcga agaaaaaaga ctctaccggc atttgcttca
     7081 ttggcgaacg taaattccgc gagttcctgg gccgttatct cccggggcaa ccgggcaaaa
     7141 tcattaccgt cgatggcgat gaaattggtg agcaccaggg gctgatgtat cacactctcg
     7201 gtcagcgtaa aggtctgggt atcggtggca ccaaagaagg caccgaagaa ccttggtatg
     7261 tggtggacaa agacgtcgaa aacaacattc tggttgtcgc tcagggccat gaacacccgc
     7321 ggctgatgtc tgtcgggttg attgcccagc agttgcactg ggtcgatcgc gaaccattca
     7381 ccggcactat gcgttgcacg gtaaaaaccc gctatcgcca gaccgatatc ccttgcaccg
     7441 tcaaggcgct ggacgatgat cgcattgaag tgattttcga tgaaccggtt gccgccgtga
     7501 cgccgggcca gtctgccgtc ttctataacg gtgaagtgtg cctcggtggc ggtattattg
     7561 agcagcgtct gccgctgccg gtctgattat tatctttact taaaacaagg aagcagtgaa
     7621 cgtggcaaag aattactatg acatcaccct cgccctggcc ggtatttgtc agtcggcacg
     7681 cctggtgcaa caactcgctc accaggggca ttgtgatgcc gatgcgctac acgtctcact
     7741 caacagtatt attgatatga accccagctc gacgctggcg gtttttggcg gtagcgaagc
     7801 caacctgcgc gtcgggctgg aaaccctgct cggcgtgctc aatgccagca gtcgccaggg
     7861 cttaaacgcc gaattaaccc gctacacact cagcttgatg gtgcttgagc gcaaactctc
     7921 ctcagcgaaa ggcgcgctcg acactctggg caaccggatc aacggcctgc aacgccagct
     7981 cgaacacttc gatttacagt ccgaaacgct gatgagcgcg atggctgcta tctatgttga
     8041 tgtgatcagc ccgcttggcc cacgcattca ggtcaccggt tcccctgctg tactgcaaag
     8101 cccacaagtg caggcgaaag ttcgcgcaac cctgctggca ggcattcgcg ccgccgtgct
     8161 ctggcaccag gtcggcggcg gacgtctgca actgatgttt tctcgtaatc gcctgaccac
     8221 tcaggcaaaa caaattcttg ctcatttaac cccggagttg tgatctatgg aattatcctc
     8281 actgaccgcc gtttcccctg tcgatggacg ctacggcgat aaagtcagcg cgctgcgcgg
     8341 gattttcagc gaatatggtt tgctgaaatt ccgtgtacaa gttgaagtac gttggctgca
     8401 aaaactggcc gcgcacgcag cgatcaagga agttcctgct tttgctgccg acgcaatcgg
     8461 ttaccttgat gcaattgtcg ccaatttcag cgaagaagat gccgcacgca tcaaaaccat
     8521 cgagcgtacc actaaccacg acgttaaagc ggttgagtat ttcctgaaag aaaaagtggc
     8581 ggagatcccg gaactgcacg cggtttctga attcatccac tttgcctgta cttcggaaga
     8641 tatcaataac ctctcccacg cattaatgct gaaaaccgcg cgtgatgaag tgatcctgcc
     8701 gtactggcgt caactgattg atggcattaa agatctcgcc gttcagtacc gcgatatccc
     8761 gctgctgtct cgtacccacg gtcagccagc cacgccgtca accatcggta aagagatggc
     8821 aaacgtcgcc taccgtatgg agcgccagta ccgccagctt aaccaggtgg agatcctcgg
     8881 caaaatcaac ggcgcggtcg gtaactataa cgcccacatc gccgcttacc cggaagttga
     8941 ctggcatcag ttcagcgaag agttcgtcac ctcgctgggt attcaatgga acccgtacac
     9001 tacccagatt gaaccgcacg actacatcgc cgagctgttt gattgcgttg cacgcttcaa
     9061 caccattctg atcgactttg atcgtgacgt ctggggttat atcgccctta accacttcaa
     9121 acagaaaacc attgctggtg agattggttc ttccaccatg ccgcataaag ttaacccgat
     9181 cgacttcgag aactccgaag ggaatctggg cctttccaac gcggtattac agcatctggc
     9241 aagcaaactg ccggtttccc gctggcagcg tgacctgacc gactccaccg tgctgcgtaa
     9301 cctcggcgtg ggtatcggtt atgcgctgat tgcgtatcaa tccaccctga aaggcgtgag
     9361 caaactggaa gtgaaccgtg accatctgct ggatgagctg gatcacaact gggaagtgct
     9421 ggctgagcca atccagacag ttatgcgtcg ctatggcatc gaaaaaccgt acgagaagct
     9481 gaaagagctg actcgtggta agcgcgttga cgccgaaggc atgaagcagt ttatcgacgg
     9541 tctggcgttg ccagaagaag agaaagcccg cctgaaagcg atgacgccgg caaactacat
     9601 tggtcgcgcc atcaccatgg ttgatgagct gaaataaact cgtatcagtg ccggatggcg
     9661 atgctgtccg gcctgcttat taagattatc cgctttttat tttttcactt tacctcccct
     9721 ccccgctggt ttatttaatg tttaccccca taaccacata atcgcgttac actattttaa
     9781 taattaagac agggagaaat aaaaatgcgc gtactggttg ttgaagacaa tgcgttgtta
     9841 cgtcaccacc ttaaagttca gattcaggat gctggtcatc aggtcgatga tgcagaagat
     9901 gccaaagaag ccgattatta tctcaatgaa catttaccgg atattgcgat tgtcgatctc
     9961 ggattgccag acgaggacgg tctgtcactg attcgccgct ggcgtagcaa tgatgtttca
    10021 ctgccgattc tggtattaac cgcccgtgaa agctggcagg acaaagtcga agtattaagt
    10081 gctggtgctg atgattacgt gactaaacca tttcatattg aagaggtgat ggcgcgaatg
    10141 caggcattaa tgcggcgtaa tagcggtctg gcttcacagg tcatttcgct accaccgttt
    10201 caggttgatc tctctcgccg tgaattatct attaatgacg aagtgatcaa actgaccgcg
    10261 ttcgaataca ccattatgga aacgttgata cgcaataatg gcaaagtggt gagcaaagat
    10321 tcgttaatgc tccaactcta tccggatgcg gagctgcggg aaagccatac cattgatgta
    10381 ctgatgggac gtctgcgcaa aaaaattcag gcacaatatc cgcaagaagt gattactacc
    10441 gttcgcggcc agggctattt gttcgaattg cgctgatgaa aaaattactg cgtctttttt
    10501 tcccgctctc gctgcgggta cgttttctgt tggcaacggc tgcggtagta ctggtgcttt
    10561 cgcttgccta cggaatggtc gcgctgatcg gttatagcgt cagtttcgat aaaactacgt
    10621 ttcggctgtt acgtggcgag agcaatctgt tctataccct tgcgaagtgg gaaaacaata
    10681 agttgcatgt cgagttaccc gaaaatatcg acaagcaaag ccccaccatg acgctaattt
    10741 atgatgagaa cgggcagctt ttatgggcgc aacgtgacgt gccctggctg atgaagatga
    10801 tccagcctga ctggctgaaa tcgaatggtt ttcatgaaat tgaagcggat gttaacgata
    10861 ccagcctctt gctgagtgga gatcattcga tacagcaaca gttgcaggaa gtgcgggaag
    10921 atgatgacga cgcggagatg acccactcgg tggcagtaaa cgtctacccg gcaacatcgc
    10981 ggatgccaaa attaaccatt gtggtggtgg ataccattcc ggtggagcta aaaagttcct
    11041 atatggtctg gagctggttt atctatgtgc tctcagccaa tctgctgtta gtgatcccgc
    11101 tgctgtgggt cgccgcctgg tggagtttac gccccatcga agccctggca aaagaagtcc
    11161 gcgaactgga agaacataac cgcgaattgc tcaatccagc cacaacgcga gaactgacca
    11221 gtctggtacg aaacctgaac cgattgttaa aaagtgaacg cgaacgttac gacaaatacc
    11281 gtacgacgct caccgacctg acccatagtc tgaaaacgcc actggcggtg ctgcaaagta
    11341 cgctgcgttc tctgcgtagt gaaaagatga gcgtcagtga tgctgagccg gtaatgctgg
    11401 agcaaatcag ccgcatttca cagcaaattg gctactacct gcatcgtgcc agtatgcgcg
    11461 gcgggacatt gctcagccgc gagctgcatc cggtcgcccc actgctggac aatctcacct
    11521 cagcgctgaa caaagtgtat caacgcaaag gggtcaatat ctctctcgat atttcgccag
    11581 agatcagctt tgtcggtgag cagaacgatt ttgtcgaggt gatgggcaac gtgctggata
    11641 atgcctgtaa atattgcctc gagtttgtcg aaatttctgc aaggcaaacc gacgagcatc
    11701 tctatattgt ggtcgaggat gatggccccg gtattccatt aagcaagcga gaggtcattt
    11761 tcgaccgtgg tcaacgggtt gatactttac gccctgggca aggtgtaggg ctggcggtag
    11821 cccgcgaaat caccgagcaa tatgagggta aaatcgtcgc cggagagagc atgctgggcg
    11881 gtgcgcggat ggaggtgatt tttggtcgcc agcattctgc gccgaaagat gaataaatat
    11941 gtccatactt cacgcattac gttaagcatc cgttataatc ggttgcagat accagcctgt
    12001 ggatgcttaa catggaatac caactcactc ttaactggcc cgattttctt gaacgtcact
    12061 ggcagaaacg cccggtggtg ttaaaacgcg gctttaataa ttttattgac ccgatctctc
    12121 cagacgagtt ggcgggtctg gcgatggaaa gcgaagttga cagtcgactg gtcagtcacc
    12181 aggatggcaa atggcaggtc agccacggcc cgttcgaaag ctacgatcat ctcggtgaaa
    12241 ccaactggtc attactggta caggcagtga accactggca tgagccgacc gccgcgctga
    12301 tgcgaccgtt ccgtgaacta ccggactggc gtattgatga tctgatgatt tctttttctg
    12361 tacctggcgg cggcgtcggc ccgcatctcg atcagtacga cgtgtttatc attcagggta
    12421 ccggacgtcg tcgctggcga gtgggcgaaa agctgcaaat gaaacagcac tgcccacatc
    12481 cggatctgtt acaggtcgat ccgttcgaag ccatcatcga tgaagagctg gagcctggtg
    12541 atattcttta tattccgcca ggattcccgc atgaaggcta cgcgctggaa aatgcgatga
    12601 actattccgt gggctttcgc gcgccaaata cgcgggaact gattagtgga tttgccgatt
    12661 atgtgctgca acgtgaactg ggcggcaact actacagcga tcctgatgtt ccacctcgcg
    12721 ctcatcctgc ggatgttctg cctcaagaga tggataaact gcgtgagatg atgctcgaat
    12781 tgatcaacca gccggaacac tttaagcaat ggtttggcga gtttatatcc cagtcacgtc
    12841 atgaactgga tatcgcgccg ccagaaccgc cttatcagcc agatgaaatc tacgatgcgc
    12901 tgaaacaagg tgatgtgctg gtgcgcctgg gtggtctgcg cgtattgcgc attggcgacg
    12961 acgtgtatgc caatggtgag aagatcgatt ccccgcaccg tccggcactg gatgcactcg
    13021 ccagcaacat tgcgctgact gcggagaatt ttggcgatgc gctggaagat ccgtcattcc
    13081 tcgcgatgct cgcggcgctg gtcaatagcg ggtattggtt cttcgaaggg taagtttgga
    13141 tttaagccgg atgcggcatc acatgccgca tcccttttcg cttacttccg ttgcgccgtt
    13201 aactcggcaa tacggacgat cacctgcacc gctttttcca taccttccag agtcacaaac
    13261 tcatgcttac catgatagtt gtaaccgcca gtgaacaagt tcgggcacgg taatcccata
    13321 aacgacaact gcgcgccgtc ggtaccaccg cggatcggtt tcagttccgg ttcaatatcg
    13381 cagtcacgca tcgcctgctg ggcgatatcg agaatatgcg gatgctcaac cactttctct
    13441 cgcatattgt agtaactgtc ttcaatcacc agttcaatgt agcaatcagg atgtaaccct
    13501 ttgcccactt ttttggcgat ctccatcatt ttacgtttac gcgcttcaaa ctgtttacgg
    13561 tcgaaatcac ggatgatgta gtgcatatcg gcccgttcaa cggtgccttt catgctcgcc
    13621 aggtgataga aaccttcata gccttctgtc atttccgggc tttcatccgc cggaacttcc
    13681 gcatgaatac gtgccgccag cgacagcgca tttaccatca ctcctttcgc cgtgcccgga
    13741 tgaacattgt taccgacaat tttgatattg accgatgcgg cgttgaagtt ttcaaactcc
    13801 agttcgccta cgccaccacc gtcaacagtg taagcccagc gggcatcgaa ggcatcaaca
    13861 tcaaaatgtt tcgccccttt gcccacttct tcatccgggg taaaggcgac tcgaatatca
    13921 ccatgcggaa tgtttttctg ttgcaatacc gccagcgcgg tcatgatttc tgcaatacct
    13981 gctttgtcat cggcacctaa caaggtttta ccatcggtgg taatcaacgt ctgacccagt
    14041 agctgatgca gcaccgggaa cataaccggt gataaaactt catcgccgat acccagcgca
    14101 atatcgccac cgcgatagtt ttcaacaatt tgcggattaa catttttgcc gctgcaatcc
    14161 ggtgaggtat ccacatgaga aataaagcca atcgccggga tatcgccagg gacgttagcc
    14221 ggtaacgtcg ccatcaaagt gcccttctca cttaaggtca cattgataag ccccatttct
    14281 tcgagctgct ctttcagcag atgcaataac ttccattggc cttccgtgct gggaacctgt
    14341 ctcacccctg cttttgattg ggtatccaga gatacgtagt tcaaaaatcg ctcaagtagt
    14401 ttatccatgt agtcaccctc actttttgtg acaacattat taagaagcaa gaaaagacaa
    14461 atattgcgtc aggtcacttt tacccctgca agcgggaata tttatcagca ttaccttaat
    14521 gaatataaag ctaagccagt aattcacaac aaagattggc gattcaagcg gtttgccgta
    14581 gaattaccgc cctcattaag agtcaccaag gtggttaacc acaaaccccg catcggtaag
    14641 ccatccgttg cgtttacatg ggacagagta aaaaattgaa taaacaaccg agttcgcttt
    14701 caccgctggt gcaattggcg ggaattcgca aatgctttga tggtaaagag gtcattcccc
    14761 agctggatct gactatcaac aatggcgagt tcctcacgct gcttggccct tctggctgcg
    14821 gtaaaacaac cgttcttcgc ctgattgcag gtctggaaac tgttgattcc ggacgcatca
    14881 tgctggataa cgaggacatc acccacgttc cggcggaaaa ccgctatgtg aacactgttt
    14941 tccaaagcta cgcacttttc ccccacatga ccgtgttcga aaatgtggcc tttgggttgc
    15001 gcatgcaaaa aacccccgct gctgaaatta cgccccgcgt gatggaagcc ctgcggatgg
    15061 tgcaactgga aaccttcgct caacgtaaac cgcatcagct ctctggtggt caacagcaac
    15121 gcgtcgccat tgctcgcgcg gtggttaaca agcctcgtct gttgttgctg gatgagtcgc
    15181 tctcagcgct ggattacaaa ctgcgtaagc aaatgcagaa cgagctgaaa gcgttacagc
    15241 gtaagcttgg tattaccttc gtctttgtca ctcacgatca ggaagaagcg ctcaccatgt
    15301 cagacaggat tgtggtgatg cgcgagggtc gtattgagca agatggtaca ccgcgcgaaa
    15361 tttacgaaga gccgaaaaac ctgtttgttg ccggcttcat tggcgaaatc aatatgttta
    15421 acgccactgt catcgaacgt ctcgacgagc agcgcgtacg cgccaacgtt gaaggccgcg
    15481 aatgtaatat ctacgttaac ttcgccgttg aaccggggca aaaactgcat gttctgctgc
    15541 gcccggaaga cttacgtgtt gaagagatta acgacgacaa ccacgctgaa gggctgattg
    15601 gttacgttcg tgagcgtaac tacaaaggca tgacgctgga gtcggttgtt gaactggaaa
    15661 atggcaagat ggtgatggtc agcgaattct tcaatgaaga cgaccctgac tttgaccact
    15721 ctctcgacca aaaaatggcc attaattggg tagaaagctg ggaggtcgta ctggctgatg
    15781 aagaacacaa gtaagttcca gaatgtagtg attgtcacta ttgtcggttg gcttgtgttg
    15841 tttgtctttc tgcccaacct gatgatcatt ggcaccagct ttttgacccg cgacgacgcc
    15901 agtttcgtca aaatggtctt tacgctggat aactacacgc gtctgctcga tccgctctat
    15961 tttgaagtgc tattgcactc gctgaatatg gcgctgatcg ccaccctcgc ctgcctggtg
    16021 ctgggctacc cgtttgcctg gtttctggcg aagctgccac acaaggtgcg tccgctgctg
    16081 ctgtttctgc tgattgttcc cttctggacc aactcactga ttcgcatcta cgggctgaaa
    16141 attttcctca gcaccaaagg ctatctcaac gagtttttgc tctggctggg cgttatcgac
    16201 acaccaattc gtatcatgtt cacgcccagt gcggtgatta tcggtctggt ttacattctg
    16261 ctaccgttta tggtgatgcc gctgtactcc agtatcgaaa aactggataa gccgttgctt
    16321 gaggcggcgc gcgatctcgg tgccagcaag ttacagactt ttatccgtat cattattcca
    16381 ctgacgatgc cgggaattat tgccggatgt ctgctggtga tgctgccagc gatgggcctg
    16441 ttctatgtat ccgacctgat gggcggtgcg aaaaacctgc tgatcggtaa cgtcatcaag
    16501 gtccagttcc tcaatattcg tgactggccg tttggtgcag ccaccagcat tacgctgact
    16561 atcgtaatgg gcctgatgtt gctggtttac tggcgcgctt ctcgtctgct gaataaaaag
    16621 gtggaactcg aatgatcggt cgactgcttc gcggcggttt tatgaccgct atctacgcgt
    16681 acctgtatat cccaatcatt attttgattg tgaactcctt taacagctcg cgctttggca
    16741 tcaactggca gggttttacc accaaatggt atagcctgct gatgaacaac gacagcctgt
    16801 tacaggcagc gcagcattca ctaacaatgg cggtgttttc ggcgacattt gctacgctta
    16861 tcggttcact gacggcagtt gcgctgtacc gttatcgctt tcgtggtaag ccgttcgtta
    16921 gcggaatgct gtttgtggtg atgatgtcac cagatatcgt gatggcgatt tctctgctgg
    16981 tgctgtttat gctgctgggt attcagcttg gcttctggtc gctgctgttc tcgcatatca
    17041 ccttctgcct gccgtttgtg gtggtgacgg tgtattcgcg cctgaaaggt tttgacgtgc
    17101 ggatgctgga agcggcgaaa gatctcggtg ccagtgaatt taccattctg cgaaaaatca
    17161 ttctgccact ggcaatgcca gcggtggcgg cgggctgggt gttaagcttt accctgtcga
    17221 tggacgatgt ggtagtttct tcgttcgtca ccggaccaag ttatgaaatt ctgccgttaa
    17281 aaatttattc gatggtcaaa gtcggcgtat cgccggaagt taacgcgctg gcaaccatat
    17341 tactggtgct gtcgctggtg atggttattg ccagccagct tattgctcgt gataaaacga
    17401 aaggtaacac aggggacgtt aaatgaaaaa atggtcacgc cacctgctcg cggcgggtgc
    17461 tctggcactg ggcatgagcg ccgctcacgc cgatgacaac aacacgctgt atttctacaa
    17521 ctggaccgag tacgtgccgc caggactgct tgaacagttc accaaagaaa ccggtattaa
    17581 ggctatctat tcgacttacg agtcgaacga aaccatgtac gccaagctga aaacgtacaa
    17641 agacggtgcc tatgacctgg tggttccttc aacctattac gtcgataaaa tgcgtaaaga
    17701 agggatgatc cagaagatcg acaagtcgaa gttaacgaac ttcagcaatc tcgatccaga
    17761 catgctcaac aagccgtttg acccgaataa cgactactcc attccgtata tctggggtgc
    17821 gacggcgatt ggcgttaacg gtgatgcggt ggatcctaaa tctgtcacca gttgggcaga
    17881 tctgtggaag ccagagtaca aaggtagcct gctgctgacc gacgatgccc gtgaagtgtt
    17941 ccagatggcg ctgcgcaaac tgggctactc cggtaacacc accgatccga aagagattga
    18001 agctgcatat aacgagctga aaaaactgat gccaaacgtc gcagcgttta actccgataa
    18061 cccggctaac ccgtacatgg aaggcgaagt taacctcggc atgatctgga acggctctgc
    18121 tttcgttgca cgccaggcgg gtacgccaat tgacgtggtg tggccgaaag aaggcggcat
    18181 tttctggatg gacagcctgg cgatcccggc aaatgccaaa aacaaagaag gcgcgctgaa
    18241 attgatcaac ttcctgctgc gcccggatgt ggcaaaacag gttgccgaaa ctatcggtta
    18301 tccaacgcca aaccttgcgg cgcgtaagct gttaagcccg gaagtggcga acgataaaac
    18361 gctctacccg gatgctgaaa ccattaaaaa tggtgaatgg cagaatgacg ttggcgcagc
    18421 cagcagcatt tatgaagagt attatcagaa gctgaaagca ggacgttaat tcagcaaacg
    18481 ggcggtggta ttaccgcccg ttttttagtt agcaaggatg tggctaatga gtcaggacag
    18541 taaagtttta tcccgctctt tcgtcggtat tgtggtcccc ttgtaggcct gataagcgta
    18601 gcgcatcagg caatgcttcc tgctttataa tcccttcagc aacttttcaa caaactcagg
    18661 caccacctgg cttgccgggc cgtaatattt ctcggcaaat tcattaccga cctgacttgg
    18721 ttcgagattc agttccacgg tgtgcgcgcc atgcagtttc gcttcgtgaa caaacccagc
    18781 cgccggataa acatgcccgg aagtaccaat ggcaatgaaa atatcagcca tcgacaacgc
    18841 catataaatt tcatccatgc cgagtggcat ttcgccaaac caaacgacgt gcgggcgcaa
    18901 gggtgccggg aactggcaac aatggcattt atcttctggc gtaacgtctc cggtccagtc
    18961 gagaacctga ccactttgcg aacaacgaac tttcagcaat tccccatgca tatgaatgac
    19021 attggtatta cctgcgcgtt catgcaggtt gtctatattc tgcgttacca gcaaaaagcg
    19081 atcgccgagg gcgtcttgca gtttagccag cgcaagatgc gcggcgttag gctgaatttc
    19141 tggctgctgc agctgtcgac gacgggcgtt ataaaacgct tgcaccagtt caggatcgcg
    19201 atcgaaacct tccggcgttg ccacatcttc aacccgatgt tcttcccaca ggccatctgc
    19261 ggcgcgaaag gtacgaatac ctgattccgc agaaattcct gcccctgtca gtacgagtac
    19321 tcttggtttt tccattgctt ccggcaccac tttatctctg aaaaaaatac gctgacgcaa
    19381 acgctcgcgc aggcggcgtt tatttttacg aaaacgactt aaccgatgac cccgacgcga
    19441 cagcatagca acctctgttg tttaatcggt tagatgtagg aaggccgcac cacgcattcc
    19501 tcccgcatca ccgtggcgcg cgcgttcaat gcgcggaaca cgagctacag gtaagagatg
    19561 acgaggcagc ctgtccgcca gttgcgttgt gattgccggg aaattcgata agccaccacc
    19621 aatgacgacc aggtcagagt caacgatggt caggatattt cccagacaaa ccgctaataa
    19681 atccagataa cgctcaacgt gcgcccttgc ctgctcatcg ccttgatcat aaagcgcaat
    19741 aatttcggga gcctgcaacg gttgatgata atagtgttgc cagagccacg caaagccccg
    19801 acctgataga taattttcga tacaaccaat ttgaccacag ccgcagcggc gtaacgggaa
    19861 atccagcccc atcatggtta acgcatcaac cggcagacgc atatggccaa actcgccggt
    19921 aatgtagctt ttaccggtaa tcggtttacc gttgaaaatc agcccgccgc caacgccggt
    19981 gccgagaatt aaccccatca ccagtggata ttgcgtaaat tcatcatccc aggcttctga
    20041 aagggcaaaa cagttggcat cgttatcaag gcgtacatcg cgatcaagac gtgcgctcag
    20101 gtcggcacgc agcggtttac cgctggcagc ggggacattg gcggcataca gcgtaccatc
    20161 ttctgtttcc ggcattcccg gaataccgat gccaacagaa cctttacagc caaaacgttg
    20221 gtcagcttcg gctaccagct cgcatactgc atctaaaaat gcgtcatagc tgtcacgcgg
    20281 tgtcggcacc cgcttttccc actgcaactg ccgaccgcta tcaaacacgc caagcgcaat
    20341 ttttgttcca ccaatatcaa acccgtaata cattgccgct ccttgtttta atgtactgcc
    20401 tttactggcc gctaaggact cgcgcagggt caatattact ggcgcgccgc gccggatacc
    20461 aacttgccaa aagactcagc aacaatgctg tgaccagtac gtagaagacg tccagccagt
    20521 gcaattccga tggcaggaag tcaataaaat agatatcgct ggagaggaac tgatgaccga
    20581 tcagcttttc aatccgctca ataatcgggg taagttgcag tgaaacaact acgccgataa
    20641 tcacaccaca caggctaccg aacagccctg ccagcaatcc ataccagaca aagatggcgc
    20701 gaattaaacc atctttcgcc cccagcgttc ttaatactgc gatatcgcca ctcttgtctt
    20761 tcaccgccat cactaaggtg gagacgatgt tgaaacaggc cacgccaatc accagtacca
    20821 tcgccagata cataatggcg cggatcatct ggatatcgcg atacatatag ccgtaagtac
    20881 caatccaact tttaatataa acatagctgt tggtcacttc acccgcatcg cgtaccagct
    20941 tattggcgtt gaaaacatcc gtcattttaa gggcaatacc tgacacgctg gaacccatat
    21001 caagatattg ttgggcatcc gccagcggga tcatggcaaa actgtgatcg agttgaccac
    21061 tcaactgcaa aataccggca atgtgcaaac gcacacgttt tggctgcatc agtttatgct
    21121 cgggattcga gttggggatc atgatcgaca cccaatcgcc ctgcttcact ttcagcgcat
    21181 ccgccacgcc tttgccgatg ataatttgct gttcgcccgc tttaaaattg cgccaggcat
    21241 ccccctgaac aaacgagggt aatgcgctca gacgctgttc ctgttgcggg ttaacgccct
    21301 tcacctgaat tgcgcgcaga ttggtaccac tttctaccag tccggtgaaa ttgatatacg
    21361 gcgcagcggc ggcaataccc ggcacttttt gcacgttatc cagtgcttcc tgccagttag
    21421 tccatggctg atttaccgcc tcaatttcac cgtgcggtac cacagcaaga atgcggttat
    21481 tcagttcgcg ttcaaagccg ttcatcgcgc ttaagccgac gatcaatacc gccacgccga
    21541 gggcaatgcc aatggtagaa atgacagaga ttagcgacac catgccgccg cgtcgccgtc
    21601 cgcgactaaa acgcaggcca atcaataacg ataaaggcat cgccattact ccgcccccat
    21661 caggctcagt tccgccgtca gacgtccatc acgcatttcc agttggcggc tcatacgttt
    21721 cgccagttgc aggtcgtgag taaccaccag gaaggcggtg ccctgcaagc gattcaattc
    21781 cccaagcaac tggaagatgc tgtcggcgtt acgcgcatcg aggttaccgg taggctcatc
    21841 cgcaagtacc agacgcggat tattgaccag cgcacgggca atcgccacac gctggcgttc
    21901 gccgccagaa agttcagatg ggcggtgatt cgcacgatgc tccagcccca ccgcttttaa
    21961 catctccagc gcacggctgt tgatttcagc gggctttttc ttgccaatca gcagtggcat
    22021 agccacgttt tccagggcag taaaatccgg cagcaggtgg tgaaactgat aaataaagcc
    22081 cagcttctgg ttgcgcagtt ctgctttcgc cgccgaagac agtttgctca tcggttgacc
    22141 gttaaagatc acatcgccgg aggttggtgt atccagcccg cccagcaggt gcagcaaggt
    22201 acttttaccg gaaccagagc taccgacgat cgccatcatt tcaccttcgc cgacgctgaa
    22261 actgacattg tgcagtacat cggtttgcac actgccttcc tgatagcgtt tgcacaggtt
    22321 gtcgcattgc aacaggatct tattcataac gtaaagcctc agcgggttga gtggcggcag
    22381 cgcgccatga agggtaaagc gtagacagca gcgcgatagc catcgccacc agcgcaataa
    22441 caatgacctg taaaggttcg atagcgaccg gcagcgccgc gccatcaagc aggacgccga
    22501 ttatcggcat cagattattt aactggctgg caagcagtgc gccaagcgcc gctccgagga
    22561 tcgcaccgat aatcccggcg ctggcccctt gcaccataaa gaccatcatg atttgtcgcg
    22621 gagttaagcc ttgcgtttgc aggatcgcga cttcgccctg cttctccatt accatcagcc
    22681 ctagtgaggt aataatgtta aacgccgcaa cggcgacaat caggctcagc agcaagccca
    22741 tcatattttt ttccatgcgt acggcctgga acagctcgcc tttacggtca cgccagtcct
    22801 gccatttgct gccttcaggc agtttttgct gacttaaaga gtcaactttc agcggctcat
    22861 ccagccacaa acgccagccg gtaatattgc ctgccggata acgcatcaga cgcgaggcat
    22921 cctcaatatt caccagcatt tcatagccat cgacttcact gttggcggcg aaagtgccaa
    22981 tcacattgaa caggcgctgg cttggaatac gccccatcgg cgtgaactgg ctggcagatg
    23041 gtaccatcac gcggatttga tcaccgcgat taacgcctag ctgtgaggca agttgttcgc
    23101 cgaggatgac attatatttc cccggctcga ggtcagtttg tttcacattg accagatacg
    23161 gcgtaagtgg atctttttgc gccgggtcga taccgagcat caccccgacc gccacgctgc
    23221 gcgcgctttg cagtaccaca tcaccggtag taataggtgc gacgcgatta acgccgtcca
    23281 gtttgactgc cgtttccggg agttgctgcg ggttaagaga gccatgctca gaagagagaa
    23341 ttgcctgtgg catcaggcca aggatgttgt tttgcagctc gcgctcaaag ccgttcatca
    23401 ctgacaatac tgtgaccagc gccatcaccc cgagggtaat gccgatggta gaaagccagg
    23461 agacgaaacg accgaagcga tccgctgcac gcccacgcat gtaacgcagg ccaataaata
    23521 gagcgacagg ttggtacatg aaatccgtct ggttgctgta gcaaagacac gagtatataa
    23581 gcgaaagcaa gatgggtaaa cgtctgaact gtaagtgatc gtcagggcta atctgaaaag
    23641 cccccgtaaa tcaatcggct aaattgccat gttttatcat caatcttgcc gcctgatctt
    23701 aatctgaaaa atcatccaat acagacagat acccattaca cctcgggtca taacccgcag
    23761 gatcaaccgc atgtaaatta aaaaagagcg tgttgtcctg atgaaacaaa aagagctatg
    23821 gattaaccag atcaaagggt tatgtatttg tctggtggtg atttatcact cggtcattac
    23881 cttttatccg catatgacca cttttcagca tccgttatcg gaagtcctga gcaaatgctg
    23941 gatctatttc aatctttacc ttgcaccctt tcgtatgccg gtttttttct ttatatccgg
    24001 ctatttgatt cgccgctata tcgacagcgt accgtgggga aattgtctcg ataaacggat
    24061 ctggaacatc ttctgggtgc tggcgctttg gggcgtggtg cagtggctgg cgttaagtgc
    24121 gctgaaccag tggctggcac ctgagcgcga tttaagcaat gcctccaatg ccgcttatgc
    24181 cgattccacc ggtgagttcc tgcacgggat gatcaccgcc agcaccagct tgtggtatct
    24241 gtatgcgtta attgtctatt tcgtggtatg taaaattttt agccgtctgg cgctgccact
    24301 attcgccttg tttgtactgc tgagtgtggc ggttaatttc gttcccacgc cgtggtgggg
    24361 aatgaacagt gtgatccgca atttgcctta ttacagcctt ggcgcatggt ttggcgcaac
    24421 aataatgacc tgtgttaaag aggtgccgtt gcgccgccat ctgctgatgg cttctttgct
    24481 taccgttctg gcggtcggtg cctggttgtt tactatctcg ctgctgttgt cgctggtatc
    24541 gattgtggtg atcatgaagc tgttttatca gtacgagcaa cgattcggta tgcgctccac
    24601 cagcctgctg aatgtgattg gctccaacac cattgctatc tacaccaccc atcgcattct
    24661 ggttgaaata ttcagcttaa ctctgcttgc gcaaatgaac gcagcacgct ggtcgccgca
    24721 agtcgaactg acactcctgc tggtttatcc ctttgtcagt ttgttcatct gtactgttgc
    24781 gggcttgctg gtaagaaaac tttcacagcg tgcattcagc gatctgttgt tctccccgcc
    24841 ttcactgccc gctgccgtca gttactctcg ttaaaccatc gccccagccg gggcgattca
    24901 atttgctaaa ccatgtcgat tacggataat gaacagcatt gcttatcaga gatatgcccc
    24961 catatgttga ggcatatcct aacgagaatc tgacaaccgt tatgcctgaa caatatcgtt
    25021 atacactgcc cgtcaaagcg ggtgagcagc gtctgctggg cgagttaacc ggcgcagcct
    25081 gtgcaacgct ggtagcggaa attgccgaac gtcacgccgg tccggtggta ctcattgcac
    25141 cagatatgca aaatgctctg cgtttgcatg atgaaatcag ccagttcacc gatcagatgg
    25201 tgatgaatct ggcggactgg gaaactcttc cctacgacag tttttcgcct catcaggaca
    25261 ttatctcctc gcgcctttcc accctttacc agctaccgac gatgcagcgt ggcgtactga
    25321 ttgttccggt gaatacgctt atgcagcgcg tttgcccaca cagttttctc cacggtcatg
    25381 cgctggtgat gaaaaaaggt cagcgcctgt cacgagatgc attacgaacc caactggaca
    25441 gcgccggtta tcgccatgtt gaccaggtga tggagcacgg cgaatacgcc acgcgcggcg
    25501 cgttgctgga tctcttcccg atggggagtg agctgcctta tcgtcttgat ttctttgatg
    25561 atgaaatcga cagcctgcgg gtgtttgacg tcgacagcca gcgcacgctg gaggaagtag
    25621 aagcgatcaa tctgctgccc gcgcacgaat ttccgaccga taaagcggca attgaactgt
    25681 tccgcagcca gtggcgcgat accttcgaag tgaagcgcga tccggaacat atttaccagc
    25741 aagtgagtaa aggcacatta cctgccggga tcgagtactg gcagccattg ttcttcagcg
    25801 aaccactgcc gccgctgttc agttatttcc ctgccaatac cttgctcgtg aatactggcg
    25861 atctggaaac cagtgccgaa cgtttccagg ctgacacgct ggcgcgtttt gagaatcgcg
    25921 gcgtcgatcc gatgcgcccg ctgttgccac cacaatcgct ctggctgcgg gtggacgagc
    25981 tcttctcaga gctgaaaaac tggccccggg tgcagctaaa aactgaacat ttaccgacaa
    26041 aagccgcgaa tgccaattta ggtttccaga aactgccaga cctggccgtt caggcacaac
    26101 aaaaagcgcc gctggatgcg ctgcgtaagt tcctcgagac tttcgacggt ccggtggtgt
    26161 tctcggtaga aagtgaaggt cgccgtgaag cgctgggtga actgctcgca cgaattaaaa
    26221 ttgctccgca acgcattatg cgtcttgatg aagccagcga ccgtgggcgt tatctgatga
    26281 ttggcgctgc cgaacatggt tttgtcgata aggtgcgtaa tctggcgctg atctgcgaaa
    26341 gcgatctgct cggtgaacgt gttgcccgcc gtcgtcagga ttctcgccgc accatcaacc
    26401 ccgatacact gatccgtaac cttgcggagc tgcatattgg tcagccggtg gtccatctgg
    26461 agcacggtgt cgggcgctat gccggaatga ccacgctcga agcgggcggc attactggcg
    26521 agtatttgat gctcacctat gccaacgacg ccaaactgta tgttccggtg tcgtcactgc
    26581 atctgattag ccgttacgca ggtggcgcgg aagaaaacgc cccgctgcat aaacttggcg
    26641 gcgatgcgtg gtcacgcgcg cggcagaaag cggcggaaaa agtgcgtgat gtggcggcgg
    26701 aattgctgga tatctacgcg caacgcgccg ccaaagaggg cttcgcgttt aaacacgatc
    26761 gtgagcagta tcagttgttc tgcgacagct tcccgtttga aaccacgccg gatcaggcgc
    26821 aggccattaa tgcggtactt agcgacatgt gtcagccgct ggcaatggat cgtctggtgt
    26881 gcggcgatgt tggctttggt aaaacagaag tggcgatgcg cgccgctttc ctggcagtag
    26941 ataaccacaa gcaggtggcg gtgttggtgc ctaccaccct tctcgcgcag cagcattacg
    27001 acaacttccg cgaccgtttc gccaactggc cggtacgtat cgaaatgatc tcccgtttcc
    27061 gcagcgccaa agagcagacg caaatccttg cggaagtggc ggaagggaaa atcgatattc
    27121 tgatcggtac gcacaaactg ctgcaaagtg acgtcaagtt taaagattta ggcctgctga
    27181 ttgtcgatga agaacaccgc ttcggggtgc gtcataaaga gcgcattaaa gcgatgcgcg
    27241 cgaacgtgga tattctgacg cttactgcaa cgccgatccc acgcacgctg aatatggcaa
    27301 tgagcggaat gcgtgacctg tcgattatcg ccacgccgcc cgcccgtcgt ctggcagtta
    27361 aaacctttgt ccgtgagtat gacagcctgg tggtccggga ggcgatcctg cgtgaaattt
    27421 tgcgcggggg gcaggtttat tatctctaca atgatgtgga aaacatccag aaagctgccg
    27481 aacggctggc agaactggtg ccagaagcgc ggatcgccat cggtcacggg cagatgcgcg
    27541 agcgcgaact ggaacgggtg atgaatgatt tccatcatca acgtttcaac gtgctggttt
    27601 gtacaaccat tatcgaaacc gggatcgaca tcccgacagc caacaccatt atcattgaac
    27661 gcgcggatca cttcggtctg gcgcagctgc accagttacg cggtcgcgtc ggacgttcgc
    27721 atcatcaggc atatgcatgg ttgctgacac cgcatccaaa agcgatgact accgatgcac
    27781 aaaaacgtct tgaagcgatt gcctcgctgg aagatctcgg ggcaggtttt gcgctggcaa
    27841 cgcacgatct ggagattcgc ggcgcgggtg aactgcttgg cgaagaacaa agcggctcaa
    27901 tggaaaccat cggtttctcg ctgtatatgg agttgctgga aaacgccgtc gatgcactga
    27961 aagccggacg cgagccgtcg ctggaagatc tcaccagcca gcaaacagaa gtcgagctgc
    28021 ggatgccgtc gctattgcca gatgatttca ttcctgacgt gaatacgcgt ttgtcgttct
    28081 ataaacgtat tgccagcgcc aaaacggaaa acgaactgga agagatcaaa gtcgagctta
    28141 tcgatcgctt cggcctgctg ccagatccgg cgcgtaccct gctggatatt gcccgtctgc
    28201 gccagcaagc gcagaaactg gggatcagga agctggaagg taatgagaaa ggcggggtga
    28261 tcgaatttgc cgagaagaat cacgttaatc cggcctggtt gattggtttg ctgcaaaaac
    28321 agccgcagca ttaccgcctt gatggtccga cgcgcctgaa atttattcag gatttgagtg
    28381 agcggaaaac gcgtatcgaa tgggtacgcc agtttatgcg tgaactggaa gagaacgcga
    28441 tcgcttaatg gtaaatcggg ggcgtttctg cgcccccatc tgcaacattt acaaattctt
    28501 tgcacttccc tgcactatcc gacactgtca ccatccataa ttcaggctta tctgtttatt
    28561 acaataacct tatatttatt attgattttt ggtgatgatc aaaacgcgtt tttctcgctg
    28621 gctaacgttt tttacgttcg ccgctgccgt ggcgctaccg gcaaaagcca acacctagcc
    28681 gctgccgcca gcgagcagtc gtctggttgg cgaaaacaaa tttcatgtgg tggaaaatga
    28741 cggtggttct ctggaagcca tcgccaaaaa atataacgtc ggctttctcg ctctgttaca
    28801 ggctaacccc ggcgttgatc cttacgtacc gcgcgcgggc agcgtgttaa cgatcccgtt
    28861 gcaaacccta cttccagacg cgccgcgcga aggcattgtg atcaacattg cggagctgcg
    28921 tctctattac tacccgccgg gtaaaaattc ggtaaccgtg tatccaatag gtattggtca
    28981 gttaggtggt gacacgctga caccgacaat ggtgaccacc gtttcagaca aacgtgcaaa
    29041 cccaacctgg acgccaacgg caaacatccg cgcccgttat aaagcacagg gaattgagtt
    29101 gcctacggta gtgccggctg gaccggataa cccaatgggc catcatgcga ttcgtctggc
    29161 ggcctatggc ggcgtttatt tgcttcacgg tacgaacgcc gatttcggca ttggcatgcg
    29221 ggtaagttct ggctgtattc gtctgcggga tgacgatatc aaaacactct ttagccaggt
    29281 caccccaggc accaaagtga atatcatcaa cactccgata aaagtctctg ccgaaccaaa
    29341 cggtgcgcgt ctggttgaag tacatcagcc gctgtcagag aagattgatg acgatccgca
    29401 gctgctgcca attacgctga atagcgcaat gcaatcattt aaagatgcag cacaaactga
    29461 cgctgaagtg atgcaacatg tgatggatgt ccgttccggg atgccggtgg atgtccgccg
    29521 tcatcaagtg agcccacaaa ctctgtaaat cctggataaa aaaaatcccc gcgacacggc
    29581 atgtcgcggg gaaaaaatca gcagtagcag gcattaatga gggttaatgc ttatttataa
    29641 attactgctg ttccatggag ggtattcgga ccggttacag aagtaatacg gaaagatttt
    29701 gcgcccatct catccgcttt ttgcgccagc tgctcttcca gcgatcccag atttgtcccc
    29761 gcgttagcac tgatagtacc gactttttgt tggccttctg gcgttgactg aacttcgaca
    29821 gccgcaaagt tggcaaatga catggagctt aaaatcgccg cagcgatgag ggtttttacg
    29881 tttttcataa tagtggcctt atgcagatga atgacgtcgc aagaaattac ttaacgatcg
    29941 ataactaaat gatagatgtg atctggatca catacaagat attttttata acaatcatta
    30001 attaatttaa aaatctttaa atttcagtta aatagaaagc aattattttt atgggatctg
    30061 gactggtgaa cggaggtgat tatttttata ataacctttc attcacagtc accaggttca
    30121 acggcatcat ggcaactgac tcaacacaat gtgtaaaaaa aagccgtggc cgcccaaaag
    30181 tgttcgacag ggatgccgcg cttgataagg ccatgaaatt gttctggcaa cacggttatg
    30241 aagcgacttc tcttgcggac ctcgtcgaag cgaccggagc caaagcgccc acgctatacg
    30301 cggaatttac caacaaagag gggttatttc gtgccgttct cgaccgctat atcgatcgtt
    30361 ttgccgctaa gcatgaagca cagctgtttt gtgaagagaa aagcgtggag tctgcgctgg
    30421 ctgactattt tgctgccatc gccaactgct ttaccagcaa agacaccccg gcaggctgct
    30481 tcatgatcaa caactgcacc accctctccc cagattcagg agatatcgcc aatacgttga
    30541 aatcacgcca tgcgatgcaa gagcgcactt tgcagcagtt tttatgtcaa cgacaagcgc
    30601 gcggggaaat cccgacccac tgtgacgtga cacatctggc agaattcctt aattgtatta
    30661 ttcaggggat gtcgatcagc gcacgcgaag gtgcatcgct ggaaaaactg atgcagattg
    30721 ccggaacgac tttgcgttta tggcccgaac tggtgaaata agtcgcaaaa aaagcccctc
    30781 agcctgagcg aatgaggggc caaattgcag agtacagcct tgttatactt tgttattcaa
    30841 aatcagttgc ccgttactat ctagcgggat ctgcgtaccc ggatcgcggt ccatgcggat
    30901 tttgccctgc tgatcgccaa tcttataggt cacatcataa ccgagcattt tttctgactt
    30961 gtcatacacc gttttacaac gctgttgcgt agtcgtgtaa gtatcgcttt cctggagaga
    31021 gccctggatc tggttaccgg catatccacc acccagcgcc cccacaacag tggcgacatc
    31081 tttaccacga ccaccaccaa actgatgccc tatcacgccg ccagcaacag cgccgagcac
    31141 cgacccggta atgcgatttt catcctgcac cggtcgacga tgggtcactg tgacgttgcg
    31201 acactcctga cgcggtgttt taaccgtttc cttgattggg gttgcagaaa caacctgagc
    31261 gtattgcggg ccccgttcaa acacgttcag actggccact gccgctacgc ccagcgcagc
    31321 tgcgacacca atcccgatac ccgccaacat tgatttattc acgggaacac ctccttcttt
    31381 gcagttatgc caatcatgca agcgaaggag aaaagcagct atcagacaga agatgaaaag
    31441 cgcgggaatg gctggaaata cttaaggatt cggagaagtc tgaaggaatg cctgatgcga
    31501 cgcttaacgc gtcttatcag gcctaccgct cgtaccattt gtccagggcg gatgaggcgt
    31561 ttatgccaca tccgccagtg tacgtcgatt aatgcaactt caaacgcgga cggataacgc
    31621 ggttaatact ccccaccagc atcattaatc cggttttaaa gtaaccatgc agcgcaatct
    31681 gatgcattcg gtatagcgag atatatacaa agcgcgcaat tcgtccttca atcatcattg
    31741 agccgcgcgt caggttaccc atcaggctac cgacagtgga gaagttcgac agcgatacca
    31801 gcgaaccatg atctttatac tgataatttt tcagcggttt accgttcatc tgcgccagaa
    31861 tattgttcat tgcgcaggtc gccatctggt gtgcagcctg agcacgcggc ggaacaaagc
    31921 ccccttccgg acgcgggcat gacgcgcagt cgccaatagc gtaaatgtct ggatcgcggg
    31981 tggtttgcag cgtcggttcc accaccagct ggttgatacg gttggtttca agaccaccga
    32041 tatctttcag gaagtctggc gctttgatcc cggctgccca caccatcaga tcagcctcaa
    32101 tatattcacc atctttagtg tgcaggccgc cttcatcagc actggtgacc atggtttgcg
    32161 tcagcacgcg aacgccaagt ttcgttagct cgttgtgggc cgcagcagag atacgtggcg
    32221 gtaatgcagg caaaatacgt tctcccgctt ctaccagcgt tacgttcagg gcttcgttgg
    32281 tcaggccttt gtaaccgtag ctgtgcagtt gcttgaccgc attgtgcaat tcagcggaga
    32341 gttctacacc cgtcgcgccg ccgccgacaa tcgcaatgtt cactttacca ttcgcgccca
    32401 ggttggcgga gtatttcagg aacagattca gcatctcctg gtggaagcga cgcgcctggt
    32461 gcgggttatc gaggaaaatg cagttctctt tgacacctgg cgtattgaaa tcgttagagg
    32521 tgctacccag cgccattacc agggtgtcat aggcgatttt acgttccgga accagcagtt
    32581 cacctttctc atcgcgcagt tctgcaatag tgattgtttt cgcttcacga tcaatatcaa
    32641 tgacggaacc cagctggaac tggaaaccat gattgcgcgc atgggccaga tagctcaacg
    32701 catcgacgcc ttcatcaagc gagccagtcg ccacttcgtg cagcagcggt ttccacagat
    32761 ggctgtggtt acgatcgacc agcgtaattt tggctttttt cttgcgtccc agcttatgcc
    32821 ccagctgtgt tgccatttcc agcccaccag caccgccgcc gacaatcaca atttttttca
    32881 atggcgtagt caacgtgacc cccttaaatt tattaaccaa ttgttaatta atggttataa
    32941 acatagctct taattaacaa caggttacag caatgaaaat gttcttcgct attgagaata
    33001 acatgaatgg tgcattggtc ataccaaaat tgatgttaat caagttttgt tgctgaaaag
    33061 ataggcgact ttaccgggtg agatcaaaaa acagagttta ttgtttatta tttgagcaag
    33121 tccggaaggg tgatataggg tgacgcgtat atcgagggcg tttggtcgtc ctgataagat
    33181 gcgcagcatc gcatcaggca ttgatgctcg actgtcggat gcggcgtaaa cgccttatcc
    33241 gacttacaga attcggagcg catcaggtac cggcaaggtt acctgatgcg acgagggcat
    33301 ttacccgagg gttttgaacg ccttaatgcg ctgcaaatgc ggggagatat tcttgaattt
    33361 gtgcgtctgc tcttcgtccc agacaatctc gtaataatga tgcaactctt cagatgtccg
    33421 ctggctgtta agcgcttcat cattacgcga caaaatcacc aggcaacgat cgcgattctt
    33481 ctcgcggaag ttggtcacac acttagtggc aatatcggca tactcttccg ggcgatcaat
    33541 cttgccttcc atgttctcat aagggaacaa attagggttg aagatcacct ggcggatatc
    33601 gcaaagaaaa ccaatccgtt ccgcccagta tccgcccaga ccaacgccgc aaatcagcgg
    33661 acgctcgtcg acgttcagtt gcaacatttt gtccacttct ttgagcagat gctgcatatc
    33721 atgtttcgga tgccgcgtac tgtagcttat caagcgtaca tccgggtcaa taaactgcaa
    33781 ttgtaagact ttctcgtggt tacccggact gttagagtca aaaccgtgta aatagataat
    33841 catcgcttcc tcacataagc cagggttagt gacctgcttt ctcttcctgc cagcgttcat
    33901 gtaactgatt cagacgggtg ctgatcgcct tccagcgagc cgagtccatc agttcctgtc
    33961 gcgaaaatga acctttatga tacaaacgtg taacacgttc tgccttgatc ggtgacagat
    34021 tatctaacac gctgacggcc cctttacgat tattgcagac caggatcata tcgcaacctg
    34081 cgtccagtga tgcctgcccg cgttcggcat aactgcccat aatcgcggca ccttccatcg
    34141 ataaatcgtc agagaaaatt acaccgtcaa aacacagttc ctgacgcaaa acggttttca
    34201 gccagtagga agaaccgctc gccggacgcg gatcaacatc actgtagatc acatgcgcag
    34261 gcataatggc gtcgagttta ttttcgcgga ttaaggaact gaagaccgac atatctttag
    34321 cgcgaatctc cgcttgtgga cgtggatcgc acggtgtttc tttgtgtgag tctgccgtta
    34381 ctgcaccgtg tcctgggaag tgtttcccgg tcgttttcat tccggcttca tgcataccat
    34441 caataaaccg gctggcaatt gccagggctt tttgtggatc ggcatgataa gaacgctcgc
    34501 caatcgccgc gctgatatgc ccgacatcca gcactggcgc aaagctgata tcaatatcca
    34561 tagcgatcat ttcgctggcc atcagccaac ccgcctcttg cgccagtttg ccgccctctt
    34621 ccattcccaa cagcgcagca aaggattgtg ctgccggtaa gcgggtaaaa ccttcgcgaa
    34681 aacgctgcac gcgtccacct tcctgatcta ccgccaccac cagatgattg cgcgatgctg
    34741 cgcggatctg gcgcaccagt tcacgtaact gggcaggatc atgatagtta cgcgtaaaga
    34801 gaatcagccc tcccaccagc ggatgcgcca gtatttcacg ctcttccgcg tccagttcgt
    34861 aaccttcgac atccaacatt actggaccca cactgctctc cttattgttt tattaatagc
    34921 tgccgccagg tgtcatcggc cagcctgata aattgttgat cgccgatttg tcgccagcgg
    34981 tactcaaacc accctgcttt gagcatcagc agccagggaa accatcgcct gacctgacgc
    35041 cataattgcg ccggataaat cttcgcgcga gtggcatagt cattgaccaa ttgccggtgc
    35101 tgttcagtat tttccaccca caccgccgcc agttccagcg cgatatcacc atctccggca
    35161 tactcccagt cgatgagttt taaccctgac gcgctatgca ctaaatttcc ggcgtggaca
    35221 tccatatgca atggacttaa gcgtaaaggc cgtggttccc gcgctttgcg cagacgtttt
    35281 aacattcgca gccaacccac agtccgccgc gccggatcgc tttgctgcca gtacagttcc
    35341 agtaagggca acagcgttat tcgccagcca aaacgtggtt gttgatgaag ataatacagc
    35401 aagcctgcca gttcgttggt atccggcaaa tacgttttta ccgcgccggg cagatagtcg
    35461 actaccatcc agtcacggag atataaatgc ggcttcggtg caatgcttgc gggtagttgt
    35521 gataaagccc gatactggcg caagaacgcg gactgcggcg catcaggatc gtgcggctga
    35581 cgcacaacaa aacgctggcc ctgatgttca atgagaaaac taccgccact aagcccacta
    35641 ttaaacgtcg tgacgggatg aaactgcggg aaaaagcgcg acagcaattc gtcgcgcgtg
    35701 atgggattat tgctgcgaaa cggcaccttt acctgaccag ataatttcgc ccgtctgcac
    35761 cagcatcagc tgcatttgta gggtcggagc gttaacgttg ccagaggcgc tggagtacag
    35821 cacgtaatga gcgccgacat tgcgggcaat gcctatggct ttactacggg tacctaaact
    35881 gtcctgcggc gacaaaccta actgttgctt agccatcgac agctgctggg cggaaaccag
    35941 ggtaaatttc ccgttattag ccagcgcatt tcgcagcgtt tcggtcgctt ctgcggcatt
    36001 cagcgaaccg ttagtacggt tgttaacgct atcaaccagc aggacgctac ccgcagtcac
    36061 cccgtcagcc ccaagcatct tactgaccat cggctgcatt gcgccattcc agtcataatg
    36121 gcgaatatgc ggcgcaggcg gtgccgtttg atcttcgtgc tcaattgggc ctggctgctg
    36181 cgggatcgtc ggcaccgagg gcactgtggg gacaggctgt tgtggctcgg ctggttgttc
    36241 cggcgctggt ttcacttctt ctaccggtgc aggttcacgt tgccccacac acccggcgag
    36301 aaacatcgcc agcgcggtaa tcaaggcgta gcgactcatt tttgtcatca agattcaccc
    36361 cttacaaata tagataaagt ctgactttgt gcgcccccag aaaattggcg ctgccgtaca
    36421 gcgttaccgc cgaatgtgcg ggaatgctaa cgctgcgtgg cctttccaga ggatgcatct
    36481 ccagccctct ggcgtcatac cagtaaaaac gataatgaac ggtaacgggt tcttgccttt
    36541 cgttataaag cgttgaggat gctgaaggtt gaatatcaga cgtcgaaagg acgggctttt
    36601 ctgcactgat gcccgcagcc aataaagatg actccatcac cagcgattgc tcatcattca
    36661 ccggaatttc cggatgtgaa cgacagccca ccagcagtaa caacgccaga gacaccagcc
    36721 caaagcatcc ttttctcatc gttacagacc tttatgcgcc agcattggtc ccagcggacg
    36781 gccacccaac aagtgcatat ggatgtggta aacctcttgt ccgccatggc ggttggtatt
    36841 catgatcaga cgatagccat cttcggcaat accttcttgc tcagcaattt ttgccgctac
    36901 ggtgatcatg cgtcccagcg cctgctcatg ctcagctgac acgtcgttca cagtcggaat
    36961 gaggatattc ggaatgatca gaatatgcgt tggcgcttgc ggcgaaatat cgcgaaacgc
    37021 cgttaccaga tcatcctggt agacgatatc ggaggggatc tcacgacgaa taattttgct
    37081 gaatatagtt tcttctgcca cgacattttc ctttttcaaa tagccatgcg ttgcgccatg
    37141 ctacgccgcg ccacactgcg agtatagagt atggtcgagt taaacctgct ctttcaacct
    37201 taactcacga tttcttaagc aaaaaatcgt tttgctgccg tatatatcgc cattattccc
    37261 atttctgcct gcgaatgcgc tgaaaagaca ttctctgttt ggtttacacg acaaatatga
    37321 atgcgcatat ttctcatttg catttacaaa caaaattatt cgcacactaa atataaccgc
    37381 tcatcaccct cctccggcat gagcctgacg acaacataaa ccaagagatt tcaaatgctt
    37441 tcaacacaat ttaacaggga taatcaatat caagccatca ccaaaccgtc actacttgcc
    37501 ggttgcatag cactggcact attaccttct gccgcttttg ctgcaccagc cactgaagaa
    37561 acggtgattg ttgagggttc agccacagct ccagatgatg gcgaaaatga ttacagcgta
    37621 acgtctacct ctgcgggtac caaaatgcag atgactcaac ttgatattcc tcagtcggtc
    37681 actattgtta gccagcagcg gatggaagat cagcagttac aaacgctggg cgaagtgatg
    37741 gaaaacacgc tggggatcag caaaagtcag gcggattccg atcgtgctct ttattattcc
    37801 cgtggattcc agatcgataa ctatatggtt gatggtatcc ccacctattt tgaatcgcgc
    37861 tggaatctgg gcgacgcact ttctgatatg gcactctttg aacgcgtaga agtagtgcgt
    37921 ggcgcgacag gactcatgac cgggacgggt aatccatctg cggcaattaa tatggttcga
    37981 aaacacgcga ccagtcgtga atttaaaggc gatgtctcgg cggaatacgg tagctggaac
    38041 aaagaacggt atgtggcgga tttacaaagc ccactcaccg aagacggtaa aatccgcgcg
    38101 cgaattgtcg gcggctacca gaataacgac tcatggctgg accgctacaa cagtgaaaag
    38161 accttcttct cgggcattgt cgatgctgat ttaggcgatc ttacgacgct ttcagccggt
    38221 tacgaatatc agcgcattga tgttaatagc ccaacctggg gcggtttacc gcgctggaat
    38281 actgatggca gcagcaacag ttacgatcgt gcacgcagta ccgcacctga ctgggcgtac
    38341 aacgataaag agatcaacaa ggtctttatg accctgaagc agcggtttgc tgatacctgg
    38401 caagcgacac tgaatgccac ccactctgaa gtcgaatttg acagcaaaat gatgtatgtc
    38461 gatgcctatg taaacaaagc ggatggtatg ctggttgggc catacagtaa ttatggacct
    38521 ggctttgatt atgtcggcgg caccggttgg aacagtggca aacgtaaagt tgatgcgctg
    38581 gatttgttcg ctgacggtag ttatgaattg tttggtcgtc agcacaatct agtgtttggt
    38641 ggcagttaca gcaaacaaaa caatcgttac ttcagttcat gggccaacat cttcccggat
    38701 gaaattggca gtttctacaa ctttaatggc aatttcccac aaaccgactg gtcaccacag
    38761 agcctggcac aggacgatac cacacatatg aaatcgttat atgctgctac tcgagtcacc
    38821 cttgccgatc cgctgcatct gatcctcggc gcacgttata ccaactggcg ggttgatacg
    38881 ctgacttaca gcatggagaa aaaccacacc acgccttacg ctggtctggt gtttgacatc
    38941 aatgacaact ggtcgaccta cgccagctat acctctattt tccagccgca aaatgatcgt
    39001 gacagttcag gcaaatatct ggctccaatc accggtaaca actacgagct gggtctgaaa
    39061 tcggactgga tgaatagccg tctgaccacc acgttagcca tcttccgtat tgagcaggat
    39121 aatgtcgctc agtccaccgg tacacctatc cccggcagca acggcgaaac cgcctataaa
    39181 gcggtggatg ggacagtcag taaaggggtg gaatttgaac tcaacggcgc aattaccgac
    39241 aactggcagc tgacatttgg cgcaacgcgc tatattgcag aggataacga aggaaacgcc
    39301 gttaatccta atctgccacg caccacggtt aaaatgttca ccagctatcg gttgcctgtc
    39361 atgccagagt tgacagtcgg cggtggtgtt aactggcaaa atcgcgtgta taccgacacc
    39421 gtgacaccgt atggcacctt ccgcgccgag caaggtagct acgcgctggt ggatctcttc
    39481 acccgctacc aggtgacgaa aaacttctcg ttacagggga acgtcaataa cctgttcgac
    39541 aaaacctacg ataccaacat ggaaggttct atcgtctacg gcgcaccgcg taatttcagc
    39601 attaccggca cgtatcaatt ctgaataaca cctgtaaaaa aggcagccat ctggcggcct
    39661 tagtctcccc aacgtcttac ggattagtgg ttacggatgt actcatccat ctcggttttc
    39721 aggttatcgg atttagtacc gaaaatcgcc tgaacaccag aaccagcaac cactacgccc
    39781 gctgcgccca gtttcttcag gccagcctga tccactttag acacatcagc aacgctgacg
    39841 cgcagacggg taatacatgc gtcgaggtta gtaatgtttt ctttaccacc aaatgcagca
    39901 accagagccg gtgccatttc gctggtacct gttgcttttg catcttcagt cgcgtcttca
    39961 cgacccggcg ttttcagatc cagtgcttta atcagcacgc ggaagatggt gtagtaaaca
    40021 atcgcataac cgataccgac gatcgggaac agccacagtt tgctgctgtt accagacaga
    40081 acgataaagt cgatcagacc gtgcgagaac gacgtaccgt cacgcatccc cagaagaata
    40141 cagattggga atgccaggcc tgccagaatc gcgtggatga tgtacaggat cggcgcaacg
    40201 aacatgaagg agaactcgat cggctcggtg ataccggtca ggaacgaggt cagcgccgcg
    40261 gagatcataa taccgcccac tttcgcgcgg ttttctggtt tagcagagtg ccagatagca
    40321 attgcggcag ctggcagacc gtacattttg aacaggaagc caccagacag tttacccgca
    40381 gtcgggtcac ccgccatata acgcggaatg tcgccgtgga aaacctgacc tgctgcgttg
    40441 gtgtattcac caatctgcat ctggaaaggt acgttccaga tgtggtgcag accaaacggc
    40501 accaggcaac gttcgatgaa accgtaaatg ccaaacgcaa ctaccgggtt ctggtaagca
    40561 gcccactgag agaaggtctg gattgcagaa ccaatcggcg gccaaatgaa ggacagcaca
    40621 acgccagtaa agatggcagc caggccagaa atgatcggca caaagcgttt accggcaaag
    40681 aagccaagat actcaggcag cttaatacgg tagaaacggt taaacatgta cgctgcgatc
    40741 gcaccggaga taatccctcc gagtacgcca gtatccgcca ggtgtttaga ggcgatttct
    40801 tcagcaggta aatgcagtac cagtggcgca accacggcca tggttttaac catgatgcca
    40861 taggcaacaa ctgcggccag cgcggatacg ccatcgttat tggtaaagcc gagggcgaca
    40921 ccgatcgcaa aaatcagtgg catgtttgca aagacggaac cgcctgcttc tgccataaca
    40981 tgcgatacaa cggcgggcag ccagctgaaa ttcgcggaac cgacgcccag cagaatacct
    41041 gcgataggca gtacggatac cggcagcatc agcgatttac cgaccttttg caggttagca
    41101 aatgcattct taaacataat tgagagtgct cctgagtatg ggtgcttttt ttacgttctc
    41161 acgcgtggca aggggggaga gcctcgccgt gtacagggca tctaagcgcc ctttatttat
    41221 tacacagagt aaaataattc agtgccgatt tgtttgacgg ctatcacgtt tcaacttcag
    41281 atcattggag aagttgcaca ttttgacaaa acatatgtca caatatttca aacgtggtta
    41341 ttcaccgccc aattttcggc ggtgaacttt actcgtttta gccattaatt acgagcttta
    41401 aaaaaactca ttcaacggat ggattgaagg cgggaagcgt cgatgtgaaa cagacgggcg
    41461 aagttatcgg tggttacctg cgccagttct tcaacggcaa cacctttcaa cacagccatg
    41521 tattctgcaa cgtcacgaac catcgcaggt tgattctctt ttcctcgatg cggtaccggc
    41581 gcaaggtaag gtgagtcagt ttccaccagt aaccgatcca gggggacata acgcgcagca
    41641 tctcgaagtt gctccgcatt acggaaggtc acaatgccgg aaaaggagat gtaaaatccg
    41701 agatccagta atttacccgc cgtttctctg tcctctgtaa aacagtgtag tacgccaccg
    41761 caatccgtca ctttttcttc gcgcagaatt gccagcgtat cggcgcgggc gtcacgggta
    41821 tggacgataa ccggcttgtt cagttcacga ccaatctgga tatgatggat gaaggactct
    41881 tgctgacgaa ctttagtttc cggtgtgtaa taataatcca gcccggtttc acccagcgct
    41941 acaacaccct cttctgccgc cagacggcgt aaatcttcta catcgtaggg atcattctgg
    42001 ttgagtggat gcacgccaca agaaaatacg acgttgtcac gttcgcctac cagatcccgc
    42061 atatgtaaat aacccggtaa tgttgtggcg actgccagac aaaatttcac atcgcgtgcg
    42121 gcggctttcg ccagaacgtc atccacgtcc ttatgcaaag attcataatc cagaccatcg
    42181 agatggcagt gtgagtcgac taaaaacatg atgtctctct taaagatgag gaaccggtag
    42241 cacaacgccc ggttgcaggt aatgctcaat acgcagtaaa agatcggtga tgagaagctc
    42301 gcggttgatg cctgtgacag acattaactg ttcacgaatg tggcaaacat cccccagtat
    42361 agcctgcagg cgcgagggag aaagatggtt tgccagttcg acgacaaggc ccggcacatc
    42421 aacattggtc acctgcgcag caccatgatg gcgttttagc gcatccatca gcaacgttgc
    42481 cagccagtgt aaacgcgccg gagcttgttc atgattaagg gccgccagca gcgaatacca
    42541 gtcgcccgat ggcacgctat atgccaatgc ctgacacaat gtttcacgag cctgccagtt
    42601 atctccctga aacaacgcca gtgccgcgcc aggcgaaccg gcgcttaagc gcaatgcggc
    42661 aagtaatgca tcctgtgaca ttgtcacttc gcgtgaaagc caggtcacgg cgtactgttc
    42721 cggcggcggc gcaaggtaat gtaaccgaca acgactacgt aatgttgcca gtaaacgttc
    42781 aggctcgcgg gtagccagga aaaaccaggt ttctgctggt ggctcttcaa gcgttttcag
    42841 caatgcgtta gccgcggcgt cggtcagtaa ggcagcatcg gttacccaga caactttcgc
    42901 accacctagg cgtgcgtgct cattcagctt ttcggtgacc tcacgtactg catcaatgcc
    42961 cagcgtattt ttcccttttt cgggagccag ggtgtagtaa tcgggatgcg tgccagcctg
    43021 catcaactga catccacgac agtgaccgca acttttgtgg ccctgcggtt gttggcagag
    43081 taaataacgg ctcagggcgt agattaaagc atcatcgccc atgcccggta acgcctgaat
    43141 gagtagcgca tggtgacctc ttccggcctg atagctggct accagttttt cgaaatcagg
    43201 tcgtaaccat ggataccatc tcatgcgtcc aactccttca cccagtgggt cacggtagtg
    43261 cggattgcat ccatcacggc ctccagcggc tgggtggcat caatggtatg aatactttta
    43321 tcttgtgcag ccagttccag atagcgggcg cgggtgcgat taaagaaatc gaaagactct
    43381 tgctcaatac gatccagctc gccgcgcgca cgcgcgcgtt ttaagccaac ttccggggta
    43441 acatcgagat agagcgttaa gtcggggcga aaatccccga gaacagcatc acgcagtgtt
    43501 gccagcatat gttggtcaat accacgtcct ccgccctgat acgcctgagt ggagagatcg
    43561 tggcgatcgc caatcaccca ggtgccgtta gccagagctg gtttgatgac cgtttctacc
    43621 agttgaacgc gcgcggcata aaacatcaga acttcggctt tatcggtaat gacttcatcg
    43681 cctaccgatt tgatatccag caccaggctt cttaactttt cggcaagttg cgtaccgcca
    43741 ggttcccgag tgaaaaccat gtcgcggata cccagttgct caagcgtctc aaccaccaca
    43801 ttacgcgcgg tagttttgcc tgcgccttcc agcccctcaa tgacgatata cttactgcgc
    43861 atttttttcc ttaagcactt tcagataatc ctgcacagac ttgttatggc tggcaagatt
    43921 ggtattaaac gtgtgaccac ctttaccatc ggccacaaaa tagagatacg gcgtttttgc
    43981 cggatgcgca gcagccttca gcgaatccgc ccccggcgta gctatcgcac ccggcggcaa
    44041 accggtaatg gtataggtgt tatacgctgt cggcgtttcc aggtctgcac gagaaagttt
    44101 gccattataa cgctctccca tcccgtaaat cacggttggg tcggtctgca ggcgcatacc
    44161 aatgcgtaaa cggttgataa ataccgaggc aacctgatcg cgttcactgg caacggcggt
    44221 ttctttttcg ataattgatg ccatcgtcac cagctgattt ttatctttat aaggcagacc
    44281 gtccgcacgc ccttcccagg cgctatcgac cgctttcacc attttcttgt gcgctcgctt
    44341 gagtaacgcg acatcggtgg tattggcggt atacatccag gtgtctggcc agaaccaacc
    44401 ttcaatccac tccgggtttt ccagttcaag tgcctgcgct acggtggcgt acttatcgtc
    44461 actcagcgtg tgcttgatat acggagcctc acgcaattgc ttgaggtagt cgctcagacg
    44521 catcccttct accagtcgca gcgggaactg tgcttcttta ccgctttcca gcaatttcag
    44581 catctcgcgc acggtcatct gcggtgtaaa gcggtaagtc ccggctttaa agtgagaaag
    44641 atccggttcg atacgcagca accattgaaa aacccgtggg cgattgatga tcttatcggc
    44701 ataaagctgt tcaccaagcg ccagacgtcc ggtccctggc ttcagggtaa atatcgtctc
    44761 ttctttgata agcaatttgc tgtcggcaag atggcgaacc ttccagacgc ccacaccagc
    44821 ggcgataccc agtaccacca gcaataacaa gataatcaat aacacttttt tcatgactaa
    44881 ttcgggcgct cacaaagtgg ggctaaatat tcatataacg ttgctgacga aaaggagaca
    44941 tcgccacagg cacgtacggg catcactggc attaacgcat tacaaataac catctcatct
    45001 gcctgcaacg cctcttccag agaggcttgc acttcgacaa gctgataaga ggattgtgcc
    45061 agcaaacgga tacagaattg tcgcataatg ccgttaacac ctgcctgatc cagacgcggc
    45121 gtataaacta cattgccctt ccgccagaac aaattagccg cacagcattc cgtaacccac
    45181 ccttcgctgt caaggaccag cgcctcatca gcgtttgtct gctcaagatg agagcggatc
    45241 aatacttgct caagccgatt aagatgttta atacctgcaa gatgaggatt gcgccccagc
    45301 cgcaccgggc ttagcgccaa cgtcatccct tcgttacgca aacggtcgta atgtgcagga
    45361 taaaccgtaa cggagagaat ccgcgttgcc ggtccactgt tcaatgtgct gtaccctcgc
    45421 ccgccactac cgcgactgat cacgactttc agcacaccat tttgctgttc tgctgccagc
    45481 gttttcatct cttgttcaag ctgaggccag aagtcacaga aaatcatcaa ccgctgacaa
    45541 gcatcctgta gtcgctggat atgcgccgat aacaaactga ctttaccgtc gataactctg
    45601 gcggtggtaa aacaaccatc accaaactgc gttgcccgat cgcttactgc cagcgattcc
    45661 tgcttatgac cgttaattaa gaacataccg gctccttatc agagaattgt tagtgtggca
    45721 gcatgttcac tacggaacaa gtcggaataa aagctaagaa aaaaggcccg caagcggacc
    45781 ttttatatgg gtgggaaatg acaacttaga tctttttaaa gatcaaagaa ccattagtgc
    45841 caccgaagcc gaaggagtta cacagcgtgt attccattcc gctgacctga cgtgcttcat
    45901 gcggtacgaa atccagatcg caaccttcat ccgggttatc caggttgatg gttggcggaa
    45961 cagcctgatc gcgcagcgcc aggatggagt agatagattc tactgcaccc gccgcaccta
    46021 acaggtgacc ggtcatagat ttcgtggagc ttaccaacac acggcttgca gcttcaccga
    46081 agatggtttt caccgcctgc gcttcagctt tatcgccagc cggcgtagaa gtaccgtgcg
    46141 cgttaacgta gccaatctga ctcgcttcaa tgcctgcatc acgcagagca tttgccatcg
    46201 ccagagctgc gcctgcgcca ttttctggcg gtgacgtcat atgataagca tcgctgctca
    46261 taccaaagcc gacgagttca gcgtaaattt tcgcaccgcg ttttttcgcg tgttcgtact
    46321 cttcaagtac cagcataccg gcaccatcgc ccagtacgaa gccatcacgc tctttatccc
    46381 acgggcggct cgccgcttgc gggttatcat tgcgggtaga taatgcacgt gccgcgccaa
    46441 aaccaccaac acccagcggc gtactggctt tctctgcgcc acctgcaacc atcacgtcag
    46501 catcgccata cgcgataata cgcgcagcat ggccaatgtt gtgcacgccg gaagtacagg
    46561 cggtcgcgat agagatgctt gggccacgca ggccatacat gatagtcaga tgacctgcca
    46621 ccatgttcac aatcgttgac ggaacgaaga atgggctgat cttacgtgga ccaccgttca
    46681 tcagagatgt gtggttttct tcgatcagtc cgaggccgcc aatcccggag ccaattgcgg
    46741 caccaatgcg ggttgcgttc tcttccgtta tatcaaggcc agaatcctgc atggcctgaa
    46801 cgccagcgac aattccatat tgaatgaagg catccatctt gcgctgttct ttgcgcgaga
    46861 taatgtcctc acagttaaaa tcctttacta agccagcaaa tttcgttgca taggcgctag
    46921 tatcgaaatg gtcgattagg ctgatgccac tctgaccggc aagcagagct ttccaggtag
    46981 actctacggt attgccgaca ggagacaaca tgcccagtcc ggtcacaact acacgacgct
    47041 tagacacgtt tgtcctccag ggagggaaaa aatgattcta gtgggacaaa aagataaaac
    47101 tcaggcggtc gaacgaccgc ctggagatgt tcacttacgc ctggtggccg ttgatgtaat
    47161 caatggcagc ctgaacggtg gtgattttct cagcttcttc gtccggaatc tcagtatcaa
    47221 actcttcttc cagagccatt accagctcaa cggtgtcaag agaatccgcg cccaggtctt
    47281 caacgaaaga agcattgttg gtaacttctt cctgcttaac gcccagctgt tcgccgataa
    47341 ttttcttaac gcgttcttcg atagtgctca tactcttaaa tttcctatca aaactcgctt
    47401 tcgcgatggt tttcgtagtg tataaaatgt tgaaaaattt gcaactaaat cccggcaggt
    47461 cttaccacga ttttacgtta ttttgaggcc ttacccccca ataacgcaaa tcattttgca
    47521 ctaattatta ctcataacca cgcagactgc gcgcaacatg agcattttgt gcaaatcgcg
    47581 gtcagaccat gtacatcccg ccgttcacat gcaaagtttc acccgtgatg taagctgctt
    47641 cgtcggatgc caggaatgca accgcgttgg cgatttcctg tgcgccgccg aggcgacccg
    47701 caggaacctg cgccaggata cccgcacgct ggtcatcgct cagcgcacgt gtcatgtccg
    47761 tttcaataaa gcccggagca acaacgttta cagtaatacc gcgtgacgca acttcgcgcg
    47821 ccagtgattt actgaagccg atcaagcccg ctttcgccgc agcgtagttg gcctgaccgc
    47881 catttcccat ggtaccaacc acagaaccga tagtgataat acgaccatga cgctttttca
    47941 tcatagcgcg cattaccgct tttgacagac ggaaaacaga tgaaaggttg gtttcgataa
    48001 tatcgttcca ctcttcatct ttcattcgca ttaacaggtt atcacgagtg ataccggcat
    48061 tattgaccag gatatccact tcaccaaatt ctgcgcgaat tttttccaga acagattcga
    48121 tagatgccgg gtcggtcaca ttcaacatca gacctttgcc gttggcacct aaataatcgc
    48181 tgatcgcctg agcgccattt tcactggtcg cagtgccaat aactttcgcg ccacgggctg
    48241 cgagcgtttc agcaattgcg cggccaattc cgcggcttgc accggttacc agtgcgattt
    48301 ttccttcaaa attcatgatt ttcctctttt aaagctcaag cgccgctgcc atcgctgaag
    48361 gttcgttcag cgccgaggcg gtcagggtgt cgacaatgcg tttcgtcagg ccagtaagca
    48421 ccttgcccgg gccgacttca tagagatgtt ctacgccttg cgctgccatg tactcaacag
    48481 acttcgtcca ctgaaccggg ttatacaact gacgtaccag tgcgtcacgg atggcatcac
    48541 cattggtttc gcatttcaca tcaacgttat tcacaacaga aactgttggt gcgttaaagg
    48601 tgattttcgc taattctact gccagtttgt cggctgctgg tttcatcagc gcacagtgag
    48661 acggtacgct cactggtaac ggcagagcac gttttgcgcc cgcagcttta caggcagcgc
    48721 cagcacgctc aaccgcttct ttatgaccgg caataaccac ctgtcccgga gagttaaagt
    48781 ttaccggaga aacgacctga cctgctgcag cttcttcaca cgctttcgca atagacgcat
    48841 catccagacc gatgattgcc gccatagcgc ccgtaccttc cggtacggct tcttgcatga
    48901 gcttgccgcg catctcaacc agacgcaccg catcagcgaa atcaatcaca ccagcgcaaa
    48961 ccagcgcgga atattccccc aggctgtgac cggccatcat tgccggtgct ttaccgccct
    49021 gctgctgcca tacgcggtac agcgcaacag aggcagtcaa cagcgctggc tgggtttgcc
    49081 aggttttatt cagttcttca gctggcccct gctgggtcag cgcccacagg tcgtagccca
    49141 gcgccgcaga agcttcagca aacgtttctt cgacaattgg atagctcgcc gccatatcag
    49201 ccagcattcc aacggtttga gaaccctgtc cagggaacac aaatgcaaat tgcgtcatgt
    49261 tttaatcctt atcctagaaa cgaaccagcg cggagcccca ggtgaatcca ccgccaaagg
    49321 cttcaagcag aaccaactgc cccggcttaa tgcgcccgtc gcgtacagct tcatccagcg
    49381 cgcacgggac agaggccgca gaggtattac cgtggcgatc cagcgtcacc acgacattgt
    49441 ccatagacat accgagtttt ttcgccgttg cactgataat acgcaggtta gcctgatgcg
    49501 gaaccagcca gtccagttga gaacggtcaa gattatttgc cgccagcgtc tcatcaacga
    49561 tgtgcgccag ttccgttacc gcaaccttga agacttcgtt gcccgccatc gtcagatgaa
    49621 ttgaattctc tggattcaca cggtcagcat taggcagcgt cagcaactca ccatagctac
    49681 cgtcggcatg cagatgggtg gagatgattc ccggctcttc agaggcagcc agcaccgcag
    49741 cgcccgcgcc atcgccaaaa ataataatag tcccacgatc ggttggatcg caggtgcgcg
    49801 ccagtacatc ggaaccgacg accagagcat acttcaccgc cccagatttc acgtattgat
    49861 cggctacgct taatgcatag gtgaaacctg cgcaggctgc tgcaacgtca aatgccgggc
    49921 aacctttaat gcccagcatg ctttgaatct gacaagctgc gctcgggaaa gcgtgcgtag
    49981 cagaagtcgt tgccacaacg atcaggccaa tctggtcttt ctcaatgccc gccatctcaa
    50041 ttgcgcgtgt cgccgcttca aagcccatgg ttgaaacggt ttcgtttggc gcggcaatgt
    50101 ggcgttcgcg gataccggta cgagtgacaa tccactcgtc agaggtgtcc accatttttt
    50161 ccaaatcggc gtttgtccgc acttgttcgg gcagatagct gccagtacca ataatcttcg
    50221 tatacatgta cgctcagtca cttttcggtt atataccgtc acttgcaaac tgcgagttcg
    50281 ctggcagcgt cctgctaccg cagagttccg cttttgccac cgtccagcag ctcaaaacca
    50341 gctgggtata cagattccag gcgagcggca attcgctgag gaacttgtcg ctgcaccgcc
    50401 tgcactgcct gttcaatcgc gaccgcaaaa gctcgctgat tggctgcacc atgacttttt
    50461 atcaccgtgc cgcgcaatcc taacagacag gcgccgttat actggtcggg gttgaggtga
    50521 ctgaatcgcc tcgtcaggct cttttgtagc caacgcttta ataacagtag ccaccacgac
    50581 cgttttttcc cttcaccctg agatttcagc agagaaagga acatcctgac aacaccttcc
    50641 atcgtcttta atgtgacatt tcctgtaaag ccgtcacaaa ccagcacatc tgtcttgcca
    50701 gttaacaact cattggcttc aagatagccg atataattga tagaagggat tgttttaagc
    50761 accgctgagg catcccgaat actgtcgaga ccctttactt cttcttcacc aatattgagc
    50821 aacgccacgc gaggattggg aatttccacc acctcttcag ccagaactga gcccataatg
    50881 gcaaattgca ccaacattgt actgtcacaa tcgacgttgg cccctaagtc aaggaccacc
    50941 gttttgccct tttgctgatg tggtaatacc gtcaccagcg ccggacgctc aatcccctcc
    51001 aggggcttga gtaataattt tgccagcccc atcagtgccc cggtattacc ggcactgaca
    51061 caggcttgcg ctcgaccttc tttcaccagc tccagggcca cgcgcattga actcccacga
    51121 ctggcgcgga tagcttgcga aggccgggca tcactggcga taactgactg cgcaggaata
    51181 atctgcagac gcgaacgttg ttcaaagtca gctttagcaa gtaatggcgt gatggcgtcg
    51241 ggattgccga ctaaaagaag agtgagttgc gaattagaat tcagtgcctg caatgctgca
    51301 ggcactgtca cggaagggcc aaaatcccct cccatgacat ctaacgccag ggttagacgt
    51361 gtcaaggtat cgtcgccgcc cggtttggtc tttccccagt tgcctgggga aaatcctcac
    51421 taagcttcat cacgcagacg cgtgattact tagcgatgac cttgcggccg cggtagtaac
    51481 cgtcggcagt gatgtggtga cgcaggtgtt tttcaccaga agttttgtct acagacaggc
    51541 tggtgactgc ggtcagcgcg tcatgggaac gacgcatgcc acgtttggaa cgggttggtt
    51601 tattctgttg tacggccatg gaccttactc ctcaattacg gtttatcccc gatccaacgg
    51661 ggagcaccaa ttacttacgc tttaagctgg ctaatacggc aaatgggttt ggcttttgca
    51721 cttcttcagg cagttcacca aagaccatgt ccgcttcgga cacttcacag tgttcagaat
    51781 catgcaccgg aactaccggc aaggcgagga tgatttcatc ttcaaccatt gcaagcagat
    51841 cgatttcacc gaattcgtta acctcaatcg gttcatacgc ttccggcagt gcttcagcct
    51901 gttcgtctga acgcacagga ctaaaacaat acgttgtgta gacctgatga gtaaacggct
    51961 tcccgcaacg ctgacactcg agcgttaccg tcaccttcgc atcgccgttt aacactgcga
    52021 gacgttggtt atcgatagcg aacgacatgg agcattccac atcactgtcc acactgacta
    52081 cggattcggc gacgcgctca acctgatcag gggtatagat accctggtaa tcaaggcgtt
    52141 tttgagccgt acgaaccgga tcgagagtca ggggtaattt taccttttgc atagggcgcg
    52201 catattaact ttgtaacgtc atagagtcaa agaaaaaggc agcctgtggt tgccttttgc
    52261 caataattcg cacacattgc gggttactgc tttattttcg ttcagtgctg ataacgaaca
    52321 tcaacctgca aaacgaagcg catagtttaa aatggctatc atcaatacgc tatatctgat
    52381 ggaaaaaaat atgcctaaac ttattttagc ctccacctcg ccatggcgtc gcgctcttct
    52441 ggaaaaactg caaatctctt ttgaatgtgc agcaccagag gtcgacgaaa ccccacgcag
    52501 cgacgaatca ccgcgacagt tggtgcttcg actggcacaa gaaaaagcgc aatctctggc
    52561 gtcacgttat ccggatcatt taattattgg ttcagaccag gtatgtgttc ttgatggcga
    52621 aatcaccggt aaaccgttaa cggaagaaaa tgcctgtctg caattacgca aagccagcgg
    52681 caatatcgtc accttctata ctggactggc gttgtttaat tcggcgaatg ggcatctaca
    52741 aacagaagtg gagccttttg acgtccattt ccgtcacctg agcgaggcgg agattgataa
    52801 ttacgtgcgt aaggagcatc ccctgcactg cgcgggtagc tttaagagtg aaggatttgg
    52861 cattacgctg tttgagcgtt tagagggacg tgaccctaac acgctggttg gtttaccgct
    52921 aatcgcgctg tgtcagatgt tacgtcggga agggaaaaac ccgctgatgg gatagttgtt
    52981 tgcgctggat gcggttacgt cgcatccggc aatcggtgca ggcggcctga tgcggcgtaa
    53041 acgccttatc aggccaaccg cccattatca ggcttatatg cttgtattag cgcgcgttac
    53101 gcagcttttg caaacaacgc ttcaaacctt catccatcgg cgcttcgata cgcatcacct
    53161 caccggtccc cggatgggta aacttcaacg ctgcggcgtg caggaacaga cgatttaatc
    53221 ccgtgcctgc ttcagtgagc tgtctgtcaa attcacggtc accgtagcga tcgtcaaagg
    53281 caatcggatg acccgcatac tgtgtatgca cacggatctg atgagtacgt ccggttaccg
    53341 ggctacaacg caccagggtg gcaaatgcat agcgttcttc cactttaaag cgtgtttccg
    53401 acggtttgcc ttcctgactg acacgcacga tacgttcgcc gctttgcaga atatttttca
    53461 ataacggcgc ttgaacgctc ttcacatgcg actgccactg accgcgcacc agcgccaggt
    53521 aatctttttg cattcctttt tcacgtaatt gctcatgcag agaacgcaac gccgagcgtt
    53581 ttttcgctat cagcaacaca cctgaagtgt cccggtcgag acgatgaacc agttcaagga
    53641 accgcgcttc cgggcgcaac gcccgcaaac cttcgataac gccgaagctt aaaccactgc
    53701 cgccatgtac cgccgtaccg gaaggtttat tcagcaccag gatatgatca tcttcatata
    53761 agatgacgtc cgccagcgcc gccacctttt gcagatgtgg cgaaaccgcc tcttcttccc
    53821 gctcagcaac gcgaaccggt gggatacgca cctcatcacc cgcttcgagt ttatattcag
    53881 gcttaatacg ttttttgttc acccgcactt cgcctttacg caaaatacgg taaatcatac
    53941 tttttggtac gcctttcaat tgggtacgca aaaagttatc gatacgttgc cccgcttcgt
    54001 cagcggtgat agcaacaatt tttacggatg gagtctctgt tttcatggtg ggcgattcta
    54061 attagccaac aggattcgcg ccactcattt ttctatgctt atatttagtt ttgcacctta
    54121 ttacttcact acgtgatcac tttattgatg gttattaaac caatcaccag caagaagtga
    54181 aaaaactgtg agtaagcggg tgataaatgg taaaagtcat cttgctataa caaggcttgc
    54241 agtggaataa tgaggccgtt tccgtgtcca tccttgttaa aacaagaaat tttacggaat
    54301 aacccatttt gcccgaccga tcatccacgc agcaatggcg taagacgtat tgatctttca
    54361 ggcagttagc gggctgcggg ttgcagtcct taccggtaga tggaaatatt tctggagagt
    54421 aatacccagt ctgtttcttt gataattgcg ctgtttttcc gcatgaaaaa cgggcaaccg
    54481 acactctgcg cctctttgag ctgacgataa ccgtgaggtt ggcgacgcga ctagacacga
    54541 ggccatcggt tcacacccgg aaaggcgtta ctttgcccgc agcttagtcg tcaatgtaag
    54601 aataatgagt aagttacgat gaaaagaatg ttaatcaacg caactcagca ggaagagttg
    54661 cgcgttgccc ttgtagatgg gcagcgtctt tatgacctgg atatcgaaag tccagggcac
    54721 gagcagaaaa aggcaaacat ctacaaaggt aaaatcaccc gcattgaacc gagtctggaa
    54781 gctgcttttg ttgattacgg cgctgaacgt cacggtttcc tcccactaaa agaaattgcc
    54841 cgcgaatatt tccctgctaa ctacagtgct catggtcgtc ccaacattaa agatgtgttg
    54901 cgtgaaggtc aggaagtcat tgttcagatc gataaagaag agcgcggcaa caaaggcgcg
    54961 gcattaacca cctttatcag tctggcgggt agctatctgg ttctgatgcc aaataacccg
    55021 cgcgcaggtg gcatttctcg ccgtatcgaa ggcgatgacc gtaccgaatt aaaagaagca
    55081 ctggcaagcc ttgaactgcc ggaaggcatg gggcttatcg tgcgcaccgc tggcgtaggc
    55141 aaatctgctg aggcgctgca atgggattta agcttccgcc tgaaacactg ggaagccatc
    55201 aaaaaagccg ctgaaagccg cccggctccg ttcctgattc atcaggagag caacgtaatc
    55261 gttcgcgcat tccgcgatta cttacgtcag gacatcggcg aaatccttat cgataacccg
    55321 aaagtgctcg aactggcacg tcagcatatc gctgcattag gtcgcccgga tttcagcagc
    55381 aaaatcaaac tgtataccgg cgagatcccg ctgttcagcc actaccagat tgagtcgcag
    55441 atcgagtccg ccttccagcg tgaagtccgt ctgccgtccg gtggttcgat tgttatcgac
    55501 agcaccgaag cgttaacggc catcgacatc aactccgcac gcgcgacccg cggcggcgat
    55561 atcgaagaaa ccgcgtttaa caccaacctc gaagctgccg atgagattgc tcgtcagctg
    55621 cgcctgcgtg acctcggcgg cctgattgtt atcgacttca tcgacatgac gcctgtacgc
    55681 caccagcgtg cggtagaaaa ccgtctgcgt gaagcggtgc gtcaggaccg tgcgcgtatt
    55741 caaatcagcc atatttctcg ctttggcctg ctggaaatgt cccgccagcg cctgagccca
    55801 tcactgggtg aatccagcca tcacgtctgc ccgcgctgct ccggtaccgg taccgtgcgt
    55861 gacaacgaat cgctgtcgct ctctattctg cgtttgatcg aagaagaagc gctgaaagag
    55921 aacacccagg aagttcacgc cattgttcct gtgccaatcg cttcttatct gctgaatgaa
    55981 aaacgttctg cggtgaatgc gattgaaacg cgtcaggacg gtgttcgctg cgtgattgtg
    56041 ccaaacgatc agatggaaac cccgcactac cacgtgttgc gcgtgcgcaa aggggaagaa
    56101 acgccgacct taagctacat gctgccgaag ctgcatgaag aagcgatggc gctgccgtct
    56161 gaagaagagt tcgctgaacg taagcgtcct gaacaacctg cactggcaac ctttgccatg
    56221 ccggatgtgc cgccagcgcc aaccccagct gaacctgccg cgactgtcgt agccccagca
    56281 ccgaaagctg caacggcaac accagcagct cctgcacaac ctgggctgtt aagccgcttc
    56341 ttcggcgcac tgaaagcgct gttcagcggt ggtgaagaag ccaaaccgac cgagcaacca
    56401 acaccgaaag cagaagcgaa accggaacgt caacaggatc gtcgcaaacc tcgtcagagc
    56461 aaccgccgtg accgtaatga gcgccgcgac acccgtagtg aacgtactga aggcagcgat
    56521 aatcgcgaag aaaaccgtcg taatcgtcgc caggcacagc agcagactgc cgagacgcgt
    56581 gagagccgtc agcaggctga ggtaacggaa aaagcacgta ccaccgacga gcagcaagcg
    56641 ccgcgtcgtg aacgtagccg ccgccgtaac gatgataaac gtcaggcgca acaagaagcg
    56701 aaggcgctga atgttgaaga gcaatctgtt caggaaaccg aacaggaaga acgtgtacgt
    56761 ccggttcagc cgcgtcgtaa acagcgtcag ctcaatcaga aagtgcgtta cgagcaaagc
    56821 gtagccgaag aagcggtagt cgcaccggtg gttgaagaaa ctgccgctgc cgaaccaatt
    56881 gttcaggaag ctccagctcc acgcacagaa ctggtgaaag tcccgctgcc agtcgtagcg
    56941 caaactgcac cagaacagca agaagagaac aatgctgata accgtgacaa cggtggcatg
    57001 ccgcgtcgtt ctcgccgctc gcctcgtcac ctgcgcgtga gtggtcagcg tcgtcgtcgc
    57061 tatcgtgacg agcgttatcc aacccagtcg ccaatgccgt tgaccgtagc gtgcgcgtct
    57121 ccggaactgg cctctggcaa agtctggatc cgctatccaa ttgtacgtcc gcaagatgta
    57181 caagttgaag agcagcgcga acaggaagaa gtacaagtgc agccgatggt gactggggtc
    57241 cctgtcgccg ccgctgtcga acctgttgtt agcgcaccgg ttgttgaaga agtggctgaa
    57301 gttgtagaag cccccgttca gattgccgaa ccgcaaccgg aagtggttga aacgacgcat
    57361 cctgaagtga ttgccgccgc agtaactgaa cagccgcagg tgattaccga gtctgatgtt
    57421 gccgtagccc aggaagttgc agaacacgca gaaccggtgg ttgaaccgca ggaagagacg
    57481 gcagatattg aagaagttgc cgaaactgct gaggttgtgg ttgctgaacc tgaagttgtt
    57541 gctcaacctg ccgcgccagt cgtcgctgaa gtcgcagcag aagttgaaac ggtagctgcg
    57601 gttgaacctg aggtcaccgt tgagcataac cacgctacgg tgccgatgac gcgcgctccg
    57661 gcaccggaat atgttccgga tgcaccgcgt cacagtgact ggcagcgccc tacttttgcc
    57721 ttcgaaggta aaggtgccgc aggtggtcat acggcaacac atcatgcctc tgccgctcct
    57781 gcgcgtccgc aacctgttga gtaataatta gctcaagtaa tcaagccctg gtaactgcca
    57841 gggctttttt atttcatctt tgaatcaaca tattaaaatt catcgaatgg catccttgct
    57901 aaccaacaat gcaaaatagg caaaaaaatg cccgccccgg cgatcccaga gcaggcagac
    57961 aaaacatacc cagtttcatg atatgtttta aagcgaaatt atttgttgag ctggaagagc
    58021 gacaatccct gcatatcggt aaatgcttta tacgatgcct gcaatgccgt ttgctgcatg
    58081 atgtaagatg aaatagttgc attccagtcc acatcaacca gatcgctcat ctgctgcgtt
    58141 tgccctaaag cgcgatcgct acctaatgaa tccagcgact ccagttcgtt cagctgcgtg
    58201 cctaattccg cgcgaacggt cagcacattg ttcagcgagt ttttcagtcc gcggttggtt
    58261 ttatctaacg ccgcagcggc ggtttcttta tccgcttcgc tatccgcgac cggcgttttc
    58321 agggctgcga tggcgctatc cagcatggca aaaagattgg tttcagaagc gctaccgtct
    58381 ggttccgcta ccgcgttact ggtaatactg tcgaaaattt tgtcgcccgt atgtcctatc
    58441 accatcgaac gcgaagcatc gacctgttgc ttaatacttt ctgttccgcc gacataatca
    58501 ccgttagcct cactaaacgg cgcagtctct gttttataac cggcaaaaat gtagcgccca
    58561 ttaccgtcag tggtgttcgc cagattcagc aactggtcac gaagcccctg aatatccgta
    58621 gccagcgagg cccggtcatc gtcactcaag gtgccattgc tggcgtagac aattttttcc
    58681 tgagcattct ggatagcagt ggtgacctgg ctaagtacac tctcttccag tgacactttt
    58741 tgagtggcga aagtacgcgc cagcgtgtac tggctgtttt gcgcctgtgc ctgggagaga
    58801 acaaccgctt gtgatgcagc aatgggatca tctgaagggt taacgactcg cttacccgtc
    58861 gacatctgtt cgccgtactt catccattct gcctgagaat tggtgatacc acgcatgttt
    58921 tgctggtaca tcatctgggt actgaaacgc attttatctc cccttagcga atgttaatca
    58981 gcgcatcaaa aatcgcgttt gccgtctgca gaacctgcgc attcgccagg taatactgct
    59041 gaaaacgttg cagatttccg tactcctcat cgagattgac accggaaatc gactgctgct
    59101 gattggaaag ctgcgtcacc acattacctt gcgtggtgct actggttttc aacgtcgcgg
    59161 ttttattacc gatatcactc actaacgagg cataagcgtc gttaaaggat ttcgcaccgc
    59221 ccaccgtttt actgttgctt tgcagatcca gcagggcctg accgttgcgg ttatcgctat
    59281 cacccgcatc tttttcgctc gccatcgcta ttttcgcttc gtcggtgatt aatacatcca
    59341 tgttgacgat ggcgtcactt actggtttca gcgtgaagct gtcgttaacg gcaggcgttc
    59401 ctgtaaacgt caactccaga ccatcaaatg ccactttacc gttggcatcc ggcgtcaccg
    59461 taaaagtggt attgctggca aggcgggtga cctgccactg attattatcg aacgagattt
    59521 tgtaatctgt cgccagtacc acggaggcat cagttaccgt ggcaccgatc gcaacgtcac
    59581 ctttgttttt cgtgttttgc agaaccgcgg gcttaccgat agtaaagaaa tcttcaccag
    59641 catcgccgtt agcatcaaat ccggctttgt gttgggtgtt gaaagcctcg gcaaatgcca
    59701 gcgccagttg tccaagcgta ttacgcgtct ggtccagctc ctgagaacgg aatgtcagaa
    59761 tgccgcccag cgacccggta ttcagtaatt tctccgggat ctcaatattg cctgccgtcc
    59821 catcaacata agcgacagtc gtacgagaag ggtcagcgct ggaaggaact gccgccagtt
    59881 gccgcgccgt acttccctga accagtgagt aaccattggc caccgtgatg ttataagtgc
    59941 cgccatcctg aacgctgact tctacaccaa caatctggtt taattcgctc accagttgat
    60001 cgcgttgatc cagcagattg ttaggtgacg cccctgcccc cacgcctgtc aggcgcgaga
    60061 tttgatcgtt caggctggca atttgtttag cgtaattgtt gatctgatca acgctggcac
    60121 ctatcgcgat attgacctgt ttgtcctggt cgcgcagata ttgatcggtg gttttaaact
    60181 gattcaccaa cccttctgat ttcccaatca gcgcctggcg cgctgccggg tcttccgcgt
    60241 tactcaccag cgtttgcagg ctggtgaaga aatcctgcat ctgtgttgcc agcgaagagg
    60301 tactggtgga gagcatattg tcgattttcg acatctgctc atagcgggcg gtcaggccgc
    60361 tactttgcgt ctgcgcagca cgtaactggt tggtaataaa cgcatcatac tcacgctgca
    60421 caccagaaac gtagacgcca ttgccaaccc agccgccagc gcccaacgtg ctattggcct
    60481 gcgccataat agtggtttgg cgggtatatc cggcaacgtt atagctggag atattattac
    60541 ttgccgtatt taacgccgcc tgggccgcgt tcagtccgct catggcgtta ttgatcaagc
    60601 tggacatgga ggttccttat aagccttcaa ttacagagta ttatcggcag cgacccgccg
    60661 gacttgagtt attcagaaca gattatcaat gttcatgctg taggttttgc tcaccttgtc
    60721 gcttatcgat ttcatctgct ggatcatgtt ggtgagttta cgggcatagt gaggatcggt
    60781 agcatacccc gcgtcctgta gggcctgcgc cccctgctcc gcactcgcgg ctgtcgtcac
    60841 ggcggcgtag cgcgggttac gtgttaacag cccaacgtaa tccgacaatg cttccagata
    60901 cgagctgtag acccgaaatt tcgcttttac tttcttcgct tcgccatttt catattcagt
    60961 cgtggtgatt tcagtaactg gacctttcca gttgccagag gctttgacgc caaacaggtt
    61021 atagctcggc tcgccgtttt cgcggcggat ttgccgttgc ccccaaccag actccagcgc
    61081 cgcctgagcg aggatcaaat gatgtggcac accgctttgc tggcttgcca gttgggcggg
    61141 cagcgagagt tgcgcgagga atgctttact gtcacccggc agcgaatcat cgtagttacg
    61201 tggcacggcc ttttgcacca gctgcgaaag cgcctgattt tgataacgca ccacagtttc
    61261 gagcgggaat ttcatcggtg ctgctggcgt ggactcctct ggcaatggtt gttctggcgt
    61321 catctgttta accatcatct ctgcaagccc cagacctttg cccgccgtca tctgttgggc
    61381 aatctgctgg tcatacatac tggtatacag gcgagtgtgc tcgctgctga acaggccatc
    61441 ttttggtaaa gcgtcgcgca tgcttttcaa catcatctgc acgaacatcc cttccacctg
    61501 acgggccacc ggacggatat ttgccgccgg atcttcgccc gctttcgcct ttagttcgtt
    61561 gagtgattgc gcatcccagg ccgcacttgc cagtagtttg ctgtcgctga tcatcagatg
    61621 atttccagtt ttgcccgcag acatcccgca ctttgcattg attgcagtat ggacatcaga
    61681 tccatcggcg tagcgcccag cgcattgagc gcgcgcacca cgttattgag gctggcgctg
    61741 gaacgtacgc tttgcagcga accgccgctc tggcgtaaat cgatctgcgt ttgtggagta
    61801 accacagtct gtccaccacc aaacggtgta tctggctggc tgacattggc ctgacgattg
    61861 actgttactg agagatttcc ctgcgctacc gcgcagctgt cgagggtcac ttcgcgattc
    61921 atcaccaccg aaccggtgcg cgagttaatc actactttag cgtcctgcgg ggtgacatta
    61981 acctgcatat tctggatatc tgcaaggaaa cggacctggg aactgttgcc acttggtacg
    62041 cgcacctgaa tagtccgcgc atctaacgcg gtggcgctgc catatccacg cacgcggttg
    62101 atggtgtcag cgatttgctg cgccatgctg aaatcttcgt cgttaagttg caaattaagg
    62161 gtattcccga cgccaaactg gctgggcaat tcacgttcaa taaccgcacc attggtgatc
    62221 cgtccaccgt tcagttggtt cacctgaaca ctgctaccgc cagcggaggc tcctgcgccg
    62281 ccaaccagaa tattgccctg cgccagcgca tacacctgac tgtcaacgcc cttaagcggc
    62341 gtcatcaaca acgtacctcc acgcaagctt ttggcatttc ccatggaaga aaccaccaca
    62401 tcgatggttt gcccctgacg tccaaacgga ggaagtgact ctgtcaccat taccgcagcg
    62461 acgtttttta gctgcatatt ggtgcccgtc ggaacggtaa ttcccagctg tgagagcatg
    62521 ttattaagcg tttgtgtggt aaacggcgtc tgggttgtct ggtcaccggt gccatccagc
    62581 cccaccacca gaccatagcc aatcagtgag ttttgcctta ccccctgaac actggtgaga
    62641 tcgcgaatac gctcagcctg agccgccgtc gtaaccagta gaagaattaa tgcagagaga
    62701 aatttaatca ccacagcctc acttacattg gcgacaggtt aaggaagaaa cgctgcaacc
    62761 agcccatatt ttgcgcttcg ttaatgtagc cattgcctac gtattcaatg cgcgcatccg
    62821 ccacctgagt agacggtacg gtattgctgc cgctgatagt gcgtggatta accacgccag
    62881 agaagcgaat aaattcggta ccctgattaa tggcaatctg tttttcaccc accacatgca
    62941 ggttgccgtt gaccagtacc tggtcaaccg tcaccgtcaa cgtgccgcta aaggtattgc
    63001 tggcattggc cccgcccttt ccgttgaacg tgttaccacc ggaggcttcg acatcggcac
    63061 gagcgttacc aaacagcccc tgcaaatagc gcggcacagt atcaaagcca aaattagttt
    63121 taccgtcacg gctggcattc gcagaggagc ttttgctggc gctgacgttc tcctgcaaca
    63181 cgatggtcag cgtatcgcca atattgcgtg gtcgacgatc ttcaaacagc ggttgatagc
    63241 catagttaat cggctgagca gactggaaaa tagaaccgtt ggcgacgggc gtcggaccgg
    63301 gaaccggttg tgcactggtc gccccctgca ccagcggcgt ggagggtatc caggcgcagc
    63361 cggttagtga aagcaccaac aagctggaaa tggcataagt atgcgcagcg tttttttgca
    63421 tggctatcat cttcaaaaat cagtaaaccg gtgaacctgc caccggttaa gccttagagt
    63481 tgcgtcagtt tttgcagcat ctgatcggtg gtggacaccg ctttactgtt gatttcgtaa
    63541 gcgcgttgca cctgaatcat attgaccagt tcttccgcca cgttgacgtt agacgtttca
    63601 acataccctt gatacagcag tcccgcgccg ttcaggcccg gcgtgctttc gttcggtaca
    63661 ccagaggatt gcgtttcggt gtagaggttt tcgccaatgc tctccagccc ggtgtcattc
    63721 ataaaggtgg tgagattgag ctgcccaacc tgaaccggag ctgcctggcc ttgttgggtt
    63781 acgctgacca cgccatcacg accgatggtg atacttaacg cattcgccgg aatggtgatc
    63841 gctggctgca cctgaaaacc accagctgcc cgttctgatc cacctggaaa gagccgtcac
    63901 gggtataggc tgatgaacca tctggcaaca tcacctggaa aaagccctgc cctttaatcg
    63961 cgacatcttt gctgttgttg gtctgcgaca ggtttccctg gctgtgtaag cgttcagtgg
    64021 cgaccgggcg tacccccgtg ccgatttgta atccggaggg taaggtggtt tgttcggaag
    64081 actgtgcccc cggctggcga atggtttgat aaagcagatc ttcaaacacc gcgcgctgac
    64141 gcttaaaacc gttagtactg acgtttgcca ggttgttggc aatgacgtcc atattggttt
    64201 gctgggcgtc aaggcccgtt ttggcgatcc ataatgaact gatcataaaa tgtcctgtag
    64261 tggattagtg acaaagtgca tatgtcttct tgccggatgc ggcgtaaacg ccttatccgg
    64321 cctacaagtg ctcgcaaaat cgataaattg cgattgcccc catagacccg ataaacttgc
    64381 gcatcgggca acttatactt gtcatgtatc ctttcaatta actcatcgac agcagttggt
    64441 tggcacggcc tgcgttatca tcgacgctgc tgatcacctt catctgcatt tcaaaacgcc
    64501 gcgcgctggc aatcatgtcg ctcattgccg caacggcatt gacgttactg ccttccagaa
    64561 cccccgacat cacacgcaag gttggatctg cctgcagtac cggccctcgc gtggcctggg
    64621 tttctgcgct taaacgaaaa atgccgtcgt caccgcgctg cacttcgctg cccgtggctt
    64681 tcaccagttt cagacgccct actggcgcaa ctgtatttgc cggatcgccc ggattgagcg
    64741 ccgagattgt gccatcggca gcaatagtga tttccgctcc ttccggcaca gcaattggcc
    64801 ccgcctcgcc tatcacctga tgcccctgaa ttgtcagttg cccggtggga tcaacctgaa
    64861 tgctgccatt acgcgtatac ccttcgctgc cgtcagcggt ctgcacggcc agccagccat
    64921 cctgctgcaa cgcgacgtcc agcgggcgcg aggtgtaatc cattttgccg ggcgtcatat
    64981 ctgcgcccgg cgttgacgcc gtgaccaacg tgcgcgtggg cagagaaagc ccttccactg
    65041 gcaccgcgcg taaagcattc aactgcgcgc gaaaaccggg cgttgaggca ttggccagat
    65101 tactggcggt taccgcctgt tgattcagtg tctggctggc tgctcccatc gcggtataaa
    65161 ttgcgtgatc cattgagcta tcccgtcagc gattagcgta agttaaccag cgtgttgagg
    65221 atctggtcct gagttttgat ggtctgggcg ttagactgat agttacgctg ggcaacgatc
    65281 atattgacca gttctttact gagatcgaca ttggacgctt ccaacgcacc gttggtcagg
    65341 gtgccaaagt tgcccgtccc ggctgtcccc aacagcgcca cgccagaaga ttgcgtcgca
    65401 gaccagacgt tgtcgccttc ggatgccaga ccttcgttgt tggcaaagtt cgccagtaca
    65461 atctgcccca gcagttgtgt ttgttcgttg gaatagttgc cgacaaccgt accgtcatca
    65521 ttgatttgat aactcaccag atcgcccggt ttgtagccat tctgggtggt tgccacaata
    65581 ttgttagcgc cggtattttg ctgcatggag ttgaggaagc tcaggctaaa cgtggcggga
    65641 tctgcgccgt taattgcgcc agtggtaata ttctctgttg gatttgaggt cagaacgcca
    65701 ttggcattaa acaccagctt cattgcaggc tcggctgtac ctgttggatc actgctatcc
    65761 tgggtgtaga cctgccagtt attatccccg gtcttcacaa agtagacgct catgtcatga
    65821 gcattaccct gactgtcgaa aacagtcacc gaaccttttt tgttatagct atccgcattg
    65881 ctggcgctga atggcgtaac agtaggaagc ggatcactgg aattcaggtt gatctgcatc
    65941 gacgccgtgg tggtagtttt cgctgccatc agggtattcg ggatcgaaat attggtcgga
    66001 ttcgcccctt gctgaatagt cggcggcgta ccggttgccg ggtaacccgt cagctgtaaa
    66061 ccttgcatat tcaccaggtt acggttttca tccagcttaa attgtccgtt acggctgtag
    66121 aacaccgaac cgttgctgtc taccagacgg aaaaaaccgt tctggctgat agcaacgtcc
    66181 agacctcgcc cggtgttggt ggtcgtgcca tcggtaaagt cctgagtgat accggcaact
    66241 tttaccccca gtcccacttt cgaaccggca aacatatcgg caaaagaggc cgtgcctgat
    66301 ttaaagccgt aggtggcgga gttggcgata ttgttgccaa taacatcgag gttggtggcg
    66361 gcagcgttta atccgctaac cgcttgagaa aaggccatga ctgactcctg aaatgtgaag
    66421 gcttaaatta tctgccgtac ttcgtcgagg gtggtggtgc cgtaagtgcc gagatccagc
    66481 gtattaccgc tgttgccgcg gatcacaccc tgcaccagag caaactgcag cggctgggca
    66541 accagttgtg taccaccgtt actggcgcta atcgctacat tgtaagaacc gttcggcgca
    66601 gtgctgccat cagtcaacgt accgtcccag gtgaaactgt ggactccggc ggtcagttca
    66661 ccaatatcaa tggtgcgcac aaccgcgcca tttttatcgg tgatggtggc cgtaactttg
    66721 tctgccgcct gttgcagctc aacaccaaac ggcgtggtcg tggtcacagc cccttcttca
    66781 ctgccggttc ccgcaagaac agtggtgccg gggatcatca cgccgtgacc gatcaggtta
    66841 ctggcctgta acgactggct gttatcaatc tgtccggaaa tagatccgag cgtggtattg
    66901 agtttttcaa tcccgctgac cgtgctgatt tgtgccaatt gcgacgtcag ctcgttgttt
    66961 tccattggat tggtcgggtc ctggtttttc agctgcgcca ccaacaaagt cagaaaactg
    67021 ctttgtaagt ctgcggcgtt gctgcccgtg agcgaactac tgctggtggt actgacgccg
    67081 gtatttgtcg gatcggtggt ggttaccgca atggacatag ctttctcctt tattgaccga
    67141 gcgtaagggt tttcagcatc atgcttttca ccatgttgag cacttcaaca ttggcctgat
    67201 agctgcgtga cgccgacatg gtgttaacca tctccccgac gacatcaacg ttcggcattt
    67261 ttacgtagcc ctttgcatcc gccagcggat tacccggttc ataaaccagt ttgtccgggg
    67321 cctgactttc tataacatcg gcaaccttta cgccgcctgt cgcagcacct ggtgcagcgt
    67381 taacctggaa taccacctgt tttgcccgat atggctgtcc atcgggaccg gtcacgctat
    67441 cagcattcgc cagattactg gccgccacgt tcaagcgctg ggactgggca gttaacgccg
    67501 acccggcgat atcaaaaata ttcagcagtg ccatccgtta atttccgctc tgtaaaacgt
    67561 tcatcatgcc tttgatttgc ccgctcaacg cgctaaggct catctggtat tgcaggctgt
    67621 tatcggcaaa ctgggtgcgt tcgcgatcca tatcgacggt attaccgtca agcgaaggct
    67681 ggtccggaat acggtattgc agttctgcgg taggaggcgt cagcgcctgc gccggaatgt
    67741 gttgcgttga ggtcatcgtc agtgcaacca cactggttgc atcccgtcca cgttgcatga
    67801 cttttttaag ttcactggca aaatcgatat cgcgcgcctg ataaccaggg gtatcggcat
    67861 tggcgatgtt tgctgccagc acttcctgac gctgggcgcg cagattgagc gcctcttgtt
    67921 gaaaacgtaa ggcggcgtcg agcttatcga gcatatctcc tccgcaggta tcaaaattct
    67981 gccatcagct taaatgcctt tacgcgcgcg ttatcggcgg aataaacgca aaatgggtcg
    68041 ctatttatgc cgttgatgac cattgcggac aggtacaatt cacgttgtag aaatggctgg
    68101 gggcgaaaat gctggcaata aaacgtagcg tggcgatcat ctcgatacta ttcactccgt
    68161 taagtgcggc gagtaatctc acatcgcaat tgcaaacctt ttttagcgcc cagctcgcgg
    68221 gggtaagtga tgaggttcgt gtttctattc gtacagcgcc caatctacta ccgccgtgcg
    68281 agcagccatt gctttcgatg agcaataatt cccgcctgtg gggcaatgtg aatgtgttgg
    68341 cacgctgcgg taacgacaaa cgatatttac aggttaatgt acaggccaca ggaaattatg
    68401 tggttgccgc gatgcccatt gtgcggggag gaaagctgga agctggcaat gtcaaactaa
    68461 aacgcggacg gcaggatacc ctgccaccgc gtacggtgct ggatatcaat caacttgttg
    68521 atgccgttag cctgcgcgat ctatcacccg atcaacctat ccagttaacc cagtttcgcc
    68581 aggcatggcg ggtaaaagcg ggacaacgcg tcaacgtgat cgccagcggt aacgggttta
    68641 gcgccaacgc agaaggtcag gcgctgaaca atgcagccgt cacacagaat gcgcgggtgc
    68701 gcatggtatc gggactggtg gtcagcggcg ttgttgatgc agatgggaat attcttataa
    68761 accagtaagc tgttaaagat tacccgtccc ttgccgataa ataagcaaca catgataaaa
    68821 gcgccctcaa tgaggaataa accatgagta ttgatcgcac ttcgcctctg aagcccgtaa
    68881 gcaccgttca accacgcgaa accactgacg cgtcggtaac gaacacccgg gcggcaaaaa
    68941 caacagcctc caccagcacc agtgtgacgt taagcgacgc gcaagcaaaa ctgatgcaac
    69001 ccggcagcag tgatatcaat cttgaacgtg tcgaagcgtt aaaactggcg attcgtaacg
    69061 gtgaactaaa aatggacacc ggcaaaattg ccgatgcgct gatcaacgaa gcgcagcaag
    69121 acttgcagag taactgatcg tatgacacgt cttgcagaga tcctcgacca gatgtccgct
    69181 gtgcttaacg atctcaaaac ggtaatggat caagaacagc aacatctctc tatggggcag
    69241 atcaacggca gccagttgca atggattaca gaacaaaaaa gctcactgct ggcgacgctg
    69301 gattacctcg aacagttacg caggaaagaa cccaatacag caaatagcgt tgatattagt
    69361 caacgctggc aggaaattac tgtgaaaacg cagcaactac gccaaatgaa tcaacataac
    69421 ggctggttac tggaaggaca gattgagcgc aatcaacagg cgctggaaat gttgaaaccg
    69481 catcaggaac cgacgctata tggggcgaac ggtcagacct caacaaccca tcgcggcggt
    69541 aaaaagattt cgatctgaat attatctccg gcctgcactg caggccggaa tgcattgtta
    69601 caccgtccgg cgggcaaatt ctttaacttt gaagcccagt accgccagtg cagcgaagta
    69661 cgcggcaatc cccgccagca cgaccgccat taaacgcagt aagcgccaag gcatggtacc
    69721 caatgaccac tccggcatga tatgtaacat acctaaaagc acgccagaca tcaccagtac
    69781 cgccaccacc agacgcaaca gaaacgccat ccagccgggt tgcggagtaa agattttctg
    69841 cttacgcaac tgccagtaaa gcagcgaagc attcagacac gccgccagac caatagaaag
    69901 tgacagcccg gcatgtttca acggaccaat aaacgccagg ttcatcaatt gcgttaaaat
    69961 cagcgtaacg atggcaattt tcactggcgt tttaatgtcc tggcgggaat aaaagccagg
    70021 agccaacact ttcactacaa tcaggccaat caaacccacc gagtaggcaa ttaacgcccg
    70081 ctgggtcatc agcgcatcaa acgcggtaaa tttaccgtac tggaacagcg aaacggttaa
    70141 cggacccgaa agaatgccca acgcaaccgc actcggcagc gccaacagga aacaaagacg
    70201 caacccccag tccatcaaac ggttgtattc atcatgattg ccactggcaa aacttttcga
    70261 cagcgacggc agcaaaatgg tgccaagcgc cacgcccagc acaccggagg gaaactccat
    70321 taagcggtcg gcgtaataca tccaagacac cgaaccggaa gcaagaaacg aggcaaaaat
    70381 ggtgttgatg attaaggaga tctggctcac agagacgcca aggatcgccg gtcccatctg
    70441 tttcaccacg cgcattgctc cggcatcgtg gaagttaatg cgcggcagga ccagcatgcc
    70501 gatcttcttc aggtgcggta gctgatacac cagctgcagg atgccgccga ccgtaacagc
    70561 ccacgccagc gccagcaccg gtgggttaaa gtacggtgcg gcaaacagcg cgaaaccaat
    70621 catgctgatg ttaagcagtg ttggcgcaaa cgccggaatc gagaagcggt tccacgtatt
    70681 cagaatcgct cccaccagcg acgccaggga gatcagcaag atatagggaa aggtaatctt
    70741 tagtagctgg ctggtcaggg caaatttgtc agctgtgtca gcgaagcctg gcgcggtcac
    70801 catgatcacc cacggtgcgg cgagcatgcc agcgaccgtc acaaccgcca gcgcaagtgt
    70861 cagcaggcca gaaacataag agacaaagac ccgcgtggcg tcttcaccct gcttactttt
    70921 atattccgcc agaatcggta caaatgcctg ggaaaatgcc ccttcggcaa agatacggcg
    70981 taacaagtta ggaagtttaa aagcgacgaa aaaggcgtcg gttgccatcc ctgcgccaaa
    71041 gattctggcg acaattgcgt ctcgtgcgaa gccaagcaca cgcgaaaaca tggtcatcga
    71101 gctgacggcg gccagcgatt ttaataaatt catcggtgtt ctaatccaga cccacgggca
    71161 aaacgcctgc aaggcaggcg ctgaaagagg cgaatagtct acctggatta tggtgaattg
    71221 ctaccgccag atgttacagg gtttattcac tcatcgcatc gcgccagatc ttgtcaacga
    71281 tacgttgcgc cagcactgcc tgttcgccgg cggtttgcag aactgtctga ttttgcacac
    71341 attcaatgaa gtgccgcgca cagccgacaa atccacgttg ctcaagagtg ctctgccaac
    71401 caggaatcgg tttatgcact acgccctgcc cgcgctcctc acgccattcg cgcatatccg
    71461 taatgtcgat gagcgcaccg tcagtcacgg cctgcacggt ttcacgctga cttccggcac
    71521 ggcgatgcat acaggtggtg atctgcaaag gatcggccga aaaatggtgc tcggcaaaca
    71581 gcatttcgcc agcgtcgtta gtcagtagcg taccgccatc cagagaggct ttgccgcccg
    71641 acaaccacag cgcggtatcc accacatgca gataatcatc cagcaacgtg aaataaagat
    71701 cgtgtggccc gacactattg ctacgatgtt tatccattct tagcgaagct gcggtagcga
    71761 gttgcgtttt taactcaccg tagagtggtg cgaaacgacg gttaaaaccg accatcaacg
    71821 tcagtttttt ccgcgccgcc agttccacca gccgttcagc atcgcgcaga ttttctgcca
    71881 gcggtttatc gacacagaca tgtacacccg cattgagtaa cgtactgacc acgtcaaagt
    71941 ggctggcggt gctggaatgc acaaaaaccg catcgcaact ggcggcaagg ctggataacg
    72001 aatcggcata aggaatgcgc cagctttcac aaattggcag ggctttcgcg cgcgtaggcg
    72061 accaggctcc ttgtaacatc cagtcagacg ctgccgccag caccggtaac cacgcttttt
    72121 gcgcaatgcc gcctaatccc acaacgccga tacgtaattt tttcacttta atctcccaga
    72181 tgggccagca acgaatcaag acgctgtttc agttctgcca cttcgatttc cagggcttcg
    72241 acgcgggcct gtaaatcacc atcaaccgcg ttcgacatat ccgttaccgc cggctgatcc
    72301 tctacctcac cactgaaaag atgcatgtag cggctttcgc gtttacccgg ttcgcgggcc
    72361 agacgcacca caaaaggacc atcttcgcga tttgccagtt gttccagcgt cgactccact
    72421 tccgccatat cgctgaattc atacattcgc gcggcgcggc tgcgcagttc acccggcgtc
    72481 tgggcaccac gcaataacaa cgtggtgatt aacgccactt ccgctgcgct cagtttcaga
    72541 tcgccaaatt ctgaattaca aaaacgttgt tcatatttgg tgacccgatt accaaagcca
    72601 ctcaccgtgc gcagataatg gcgtttgacc agattatcca gttgctcctg cacttcagat
    72661 tcactcagat tcatgaccgg ttcacggttc gttttctgat tacaggccgt gactacacca
    72721 ttgactgaga gcgggtattg ctccggcgtg gtcacctgct tttccagcaa gcaaccaatc
    72781 acgcgagctt caagggcagt aagttgatat ttcatgagat tctccttagc ggccgggcgt
    72841 ccagtctggg gtagttaatg ccgtcagtac gtgatcgcgc cattgtccat caatcaacag
    72901 atagtctttc gcatagcctt ctttttcaaa acccagtcgc gccagtaaat caccgctgcg
    72961 tttattgtgc ggcatataat tagccataat gcgatgaata tgttgggtgc gctgcatata
    73021 acgaatggct gcggtcaggg cttcaaacat gagtcctttg ccctgccatt tttgcccaat
    73081 cgaataaccg agatagcagg catgaaaaga gccacgaaca acattggaaa aattggcaac
    73141 gccaataatc tctttttcgt ccgggtcgaa taagccaaag tagaaagctg aaccttgttt
    73201 atgaaattcg ttaatcatcc ccagcctggc ctgccagcct gatggataac agtggctttc
    73261 gtcgcgcact ggctcccagg gcttgaggaa atggcgattc tctgcgtaat aatccgcaag
    73321 acgccaggca tcacgatcat gcaccagacg cacgaccagt cggtctgtgg ttaagcgcac
    73381 ttttggcacg ttactgcgat agccaaacat tctataccta ctccttcccg taacgtcttt
    73441 aatacgctgt aagtattact atacctgcgc aaccagaccc tgtgaaaagt ccaaaatgcc
    73501 attttctgca ttattcacat atttgataaa aactctctat caaagcgacg ttttgataaa
    73561 aaaatattgt ccccgaagtg atgggaaaag tcttagcggc aatgcagaat agaaagtgct
    73621 catcttttat ttcccgggag gggaaatgtc ccgcgtatcg caggcgagga acctgggtaa
    73681 atatttcctg ctcatcgata atatgctggt cgtgctgggg ttctttgttg tcttcccgct
    73741 gatctctatc cgcttcgttg atcaaatggg ctgggccgcc gtcatggtcg gtattgctct
    73801 cggtctacgc cagtttattc agcaaggtct gggtattttc ggcggcgcaa ttgccgaccg
    73861 ctttggtgcc aaaccgatga ttgttaccgg tatgctgatg cgcgccgccg gattcgccac
    73921 aatgggtatc gcccacgaac cgtggctatt gtggttttca tgcctgctct cgggactcgg
    73981 cggcacgttg tttgatccgc cgcgttcggc gctggtggtg aaactaatcc gtccacaaca
    74041 acgttgtcgt tttttctcgc tgttgatgat gcaggacagt gccggtgcgg tcattggcgc
    74101 attgttgggg agctggctgt tgcaatacga ctttcgcctg gtctgcgcca caggggccgt
    74161 tttgtttgtg ctgtgtgcgg cgttcaatgc gtggttgtta ccggcatgga aactctccac
    74221 cgtacgcacg cccgttcgcg aaggcatgac ccgcgtgatg cgtgacaagc gttttgtcac
    74281 ctatgtcctg acgctggcgg gttactacat gctggctgta caagtgatgc tgatgctgcc
    74341 aattatggtc aacgacgtgg ctggcgcgcc ctctgccgtt aaatggatgt atgccattga
    74401 agcgtgtctg tcgttaacgt tgctctaccc tatcgcccgc tggagtgaaa agcattttcg
    74461 tctggaacac cggttgatgg ctgggctgtt gataatgtca ttaagcatga tgccggtggg
    74521 catggtcagc ggtctgcaac aacttttcac cctgatttgt ctgttttata tcgggtcgat
    74581 cattgccgag cctgcgcgtg aaaccttaag tgctttgctg gcggacgcaa gagctcgcgg
    74641 cagctatatg gggtttagcc gtctgggtct ggcgattggc ggcgctattg gttatatcgg
    74701 tggtggctgg ctgtttgacc tgggcaaatc ggcgcaccag ccagagcttc cgtggatgat
    74761 gctgggaatt attggcatct tcactttcct tgcgctgggt tggcagttta gtcagaaacg
    74821 cgcaacgcgt cgtttgctgg aacgcgacgc ctgatttgat actcccccct gactcctcca
    74881 tactgaaagt gaactgacag ccaaatggag gagtcacgtg aagctataca tttacgatca
    74941 ctgcccttac tgcctcaaag cccgcatgat tttcggcctg aaaaatatcc ccgtcgaatt
    75001 acatgttctg ctcaacgacg acgcagaaac acccacccgg atggtcggtc aaaaacaggt
    75061 tcccattctg caaaaagatg acagccgcta tatgccagaa agcatggata tcgttcacta
    75121 tgtcgataaa ctcgacggca aaccgttact gaccggcaaa cgttcccctg ccattgaaga
    75181 gtggctgcgc aaggtcaatg gctacgccaa caaactgctg ttgccgcgtt ttgccaaatc
    75241 ggcatttgat gagttttcta ctcccgccgc gcgcaaatat ttcgtcgaca agaaagaggc
    75301 cagcgcgggt aattttgccg acctgctggc ccactctgac ggtctgatta agaatatcag
    75361 cgatgatttg cgtgcgctgg acaaactgat cgtcaaaccg aacgccgtga atggcgaact
    75421 ttcggaagat gatattcagc tatttccgct actgcgtaat ctgacgctgg tagccggaat
    75481 caactggcca agccgcgttg ctgattaccg cgataatatg gcgaaacaga cacaaatcaa
    75541 tttgttatca tcaatggcga tttaatcctg acccgccgcg gcagcctgta ttcgcgggtt
    75601 gccgccgttt tcagtttttt ctgctggcga gtttatcgct ggtgacacat gctgcgttgt
    75661 gtcaggatat tgacgcagat ttcatgtcga ggactaccat gaacaagttt ttatttgccg
    75721 ctgcattgat cgtcagtggc ctgctcgttg gctgtaatca actcacccaa tacaccatca
    75781 ccgaacaaga aattaaccag tcgcttgcga aacataataa tttctcaaaa gatatcggtt
    75841 tacccggcgt ggctgacgcc catattgttc tgacaaactt gatcagccaa attggtcgcg
    75901 aagagccgaa taaggttacc ctaaccggag acgccaatct ggacatgaac tccctgttcg
    75961 gtagtcagaa agcgaccatg aaactgaagc tgaaagcgct gcccgtgttt gataaagaaa
    76021 aaggtgcgat cttcctgaaa gagatggaag tggtcgatgc gacggtacaa ccggaaaaaa
    76081 tgcaaacggt gatgcaaacg ttgcttccct atttgaacca ggcattacgc aattacttta
    76141 accagcaacc tgcttacgtc ctgcgcgaag atggcagtca gggcgaagca atggcgaaaa
    76201 aactggcgaa aggcattgaa gtgaagccag gcgaaattgt cattccgttt acggattaat
    76261 gacgagggcg cattcgcgcc ctttattttt cgtgcaaagg aaaacgtttc cgcttatcct
    76321 ttgtgtccgg caaaaacatt ccttcagccg gagcatagag attaatgact gcaccatccc
    76381 aggtattaaa gatccgccgc ccagacgact ggcaccttca cctccgcgat ggcgacatgt
    76441 taaaaactgt cgtgccgtat accagcgaaa tttatggacg ggctatcgta atgcccaatc
    76501 tggctccgcc cgtgaccaca gttgaggctg ccgtggcgta tcgccaacgt attcttgacg
    76561 ccgtacctgc cgggcacgat ttcaccccat tgataacctg ttatttaaca gattcgctgg
    76621 atcctaatga gctggagcgc ggatttaatg aaggcgtgtt caccgctgca aaactttacc
    76681 cggcaaacgc aaccactaac tccagccacg gcgtgacgtc aattgacgca atcatgccgg
    76741 tacttgagcg catggaaaaa atcggtatgc cgctactggt gcatggtgaa gtgacacatg
    76801 cagatatcga catttttgat cgtgaagcgc gctttataga aagcgtgatg gaacctctgc
    76861 gccagcgcct gactgcgctg aaagtcgttt ttgagcacat caccaccaaa gatgctgccg
    76921 actatgtccg tgacggaaat gaacggctgg ctgccaccat cactccgcag catctgatgt
    76981 ttaaccgcaa ccatatgctg gttggtggcg tgcgtccgca cctgtattgt ttacccatcc
    77041 tcaaacgcaa tattcaccaa caggcattgc gtgaactggt cgccagcggt tttaatcgag
    77101 tattcctcgg tacggattct gcgccacatg cacgtcatcg caaagagagc agttgcggct
    77161 gcgcgggctg cttcaacgcc ccaaccgcgc tgggcagtta cgctaccgtc tttgaagaga
    77221 tgaatgctct gcagcacttt gaagcattct gttctgtaaa cggcccgcag ttctatgggt
    77281 tgccggtcaa cgacacattc atcgaactgg tacgtgaaga gcaacaggtt gctgaaagca
    77341 tcgcactgac tgatgacacc ctggtgccat tcctcgccgg ggaaacggta cgctggtccg
    77401 ttaaacaata aaaatcgtag cttcctgttg tcattaggtt atttaacctg tataaataac
    77461 cagtatattc aacagggggc tattatgcga attgaagtca ccatagcgaa aacttctcca
    77521 ttgccagctg gggctattga cgccctggct ggcgaacttt cccgccgtat tcagtatgcg
    77581 tttcctgata atgaaggcca cgtatcggta cgttatgccg cagcgaataa tttatcggtt
    77641 attggcgcaa caaaagaaga taaacagcgc attagcgaaa ttctccagga aacgtgggaa
    77701 agcgccgatg actggtttgt cagcgaataa tatgcagtga tttttgtttt tgccgggtcg
    77761 ccccggcttt tttattttac ttacgaattt tgagtacaaa atttctacac ttcctcaaaa
    77821 taattgcaca atatgctaaa aaaatatgaa tgacatgctg ttttattgtt catataaatc
    77881 ttaaaagcag aaatttatct gttcccccct ttaaaaattc ggtgaatacc cttacttatt
    77941 ggtatactga agttgcttca gataaaaacg cattgaacct cgaataacgt tgtctagtaa
    78001 cacgaattag ggggccatga tggaaaaaaa taatgaagtc attcagactc atccgctcgt
    78061 agggtgggac atcagcaccg ttgatagcta tgatgcgctg atgttgcgtt tgcactacca
    78121 gaccccaaat aagtccgagc aggaagggac tgaagttggt caaacgctct ggttaaccac
    78181 tgatgttgcc agacaattta tttcgatatt agaagcagga atcgccaaaa ttgaatccgg
    78241 tgatttccag gtaaacgagt atcggcgtca ttaacatgac atgaccatcc acccaaaagg
    78301 cacctcaccg gtgcctttac catttctggg ttgtatatct gcattcagtt aattactctg
    78361 cttaaaccat gagagcagag agaaaaaaat gaaatacgat ctcatcatta ttggcagcgg
    78421 ttccgtaggc gctgccgccg ggtattatgc aacccgcgcc ggtttaaacg tgctaatgac
    78481 cgacgcccat atgccaccgc atcaacacgg cagccaccac ggcgatacgc gattaattcg
    78541 ccatgcttat ggtgaaggcg aaaagtatgt cccgctggtc ctccgcgcgc aaacgctgtg
    78601 ggatgaactt tcccgccaca acgaagatga tcccattttt gtacgctctg gtgtcattaa
    78661 cctcggcccg gctgactccg catttctcgc caacgtcgcc cacagcgccg aacagtggca
    78721 actcaacgtt gaaaagctca atgcgcaagg gattatggcc cgctggccag aaatacgcgt
    78781 cccggacaac tacatcggct tatttgagac tgattccggt tttttgcgca gcgaactggc
    78841 gattaaaacc tggatccaac tggcgaagga agcgggctgt gcgcaactgt tcaactgccc
    78901 ggtcaccgca attcgtcatg acgatgatgg cgtaactatt gaaacggttg acggtgagta
    78961 tcaggcgaaa aaagcgattg tctgcgcggg aacatgggta aaagacctgc tcccggagca
    79021 gcctgtccag cccgtacgta aagtatttgc ctggtatcag gccgatggcc gctatagcgt
    79081 gaagaataaa ttcccggcgt ttaccggtga actgcccaat ggcgatcaat attatggttt
    79141 tccggcagaa aacgacgcgt tgaagattgg caaacataac ggaggccagg ttatccattc
    79201 agcggatgaa cgtgttccgt ttgcggaagt ggtcagcgat ggttcggaag ccttcccgtt
    79261 cttgcgcaat gtattgccgg gtatcggttg ctgcctgtac ggcgctgcct gcacctatga
    79321 taattcgcct gacgaagatt ttattatcga taccctaccc ggccacgata atacactgct
    79381 cattaccggc ctgagtgggc acggttttaa atttgcgtca gttttagggg aaatagctgc
    79441 cgattttgcg caagacaaaa aaagcgattt tgatttgacg ccattcaggc tttcccgctt
    79501 ccaataatca tcattacggc ctccagaatg aggccgtttt tacaggagta tttaatgcgt
    79561 cggctgttgc actatctca
//
LOCUS       DAESHI010000004        72510 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.004, whole genome shotgun sequence.
ACCESSION   DAESHI010000004 DAESHI010000000
VERSION     DAESHI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72510
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.004"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..215)
                     /gene="tuf"
                     /locus_tag="KW011_000259"
     CDS             complement(<1..215)
                     /gene="tuf"
                     /locus_tag="KW011_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBH7658702.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(286..2400)
                     /gene="fusA"
                     /locus_tag="KW011_000260"
     CDS             complement(286..2400)
                     /gene="fusA"
                     /locus_tag="KW011_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBH7658703.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="KW011_000261"
     CDS             complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="KW011_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBH7658704.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="KW011_000262"
     CDS             complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="KW011_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBH7658705.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="KW011_000263"
     CDS             complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="KW011_000263"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HBH7658706.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="KW011_000264"
     CDS             complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="KW011_000264"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HBH7658707.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="KW011_000265"
     CDS             complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="KW011_000265"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HBH7658708.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4604..5326)
                     /locus_tag="KW011_000266"
     CDS             complement(4604..5326)
                     /locus_tag="KW011_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH7658709.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="KW011_000267"
     CDS             complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="KW011_000267"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBH7658710.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6526..6744
                     /locus_tag="KW011_000268"
     CDS             6526..6744
                     /locus_tag="KW011_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HBH7658711.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="KW011_000269"
     CDS             complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="KW011_000269"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBH7658712.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7478..7678)
                     /locus_tag="KW011_000270"
     CDS             complement(7478..7678)
                     /locus_tag="KW011_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HBH7658713.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="KW011_000271"
     CDS             complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="KW011_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HBH7658714.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="KW011_000272"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="KW011_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HBH7658715.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="KW011_000273"
     CDS             10175..12088
                     /locus_tag="KW011_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH7658716.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="KW011_000274"
     CDS             12088..13110
                     /locus_tag="KW011_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBH7658717.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="KW011_000275"
     CDS             13104..13322
                     /locus_tag="KW011_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HBH7658718.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="KW011_000276"
     CDS             13376..14245
                     /locus_tag="KW011_000276"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HBH7658719.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..14704)
                     /locus_tag="KW011_000277"
     CDS             complement(14300..14704)
                     /locus_tag="KW011_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HBH7658720.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15006..15638
                     /gene="crp"
                     /locus_tag="KW011_000278"
     CDS             15006..15638
                     /gene="crp"
                     /locus_tag="KW011_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HBH7658721.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGSR"
     gene            15689..17779
                     /locus_tag="KW011_000279"
     CDS             15689..17779
                     /locus_tag="KW011_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBH7658722.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17846..19066)
                     /gene="argD/dapC"
                     /locus_tag="KW011_000280"
     CDS             complement(17846..19066)
                     /gene="argD/dapC"
                     /locus_tag="KW011_000280"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HBH7658723.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="KW011_000281"
     CDS             complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="KW011_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HBH7658724.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19747..20349)
                     /locus_tag="KW011_000282"
     CDS             complement(19747..20349)
                     /locus_tag="KW011_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HBH7658725.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(20339..20506)
                     /locus_tag="KW011_000283"
     CDS             complement(20339..20506)
                     /locus_tag="KW011_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HBH7658726.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="KW011_000284"
                     /gene_synonym="rotA"
     CDS             complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="KW011_000284"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HBH7658727.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21454..22635
                     /gene="tsgA"
                     /locus_tag="KW011_000285"
     CDS             21454..22635
                     /gene="tsgA"
                     /locus_tag="KW011_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HBH7658728.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22897..25440
                     /gene="nirB"
                     /locus_tag="KW011_000286"
     CDS             22897..25440
                     /gene="nirB"
                     /locus_tag="KW011_000286"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HBH7658729.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25437..25763
                     /gene="nirD"
                     /locus_tag="KW011_000287"
     CDS             25437..25763
                     /gene="nirD"
                     /locus_tag="KW011_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HBH7658730.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25889..26695
                     /gene="nirC"
                     /locus_tag="KW011_000288"
     CDS             25889..26695
                     /gene="nirC"
                     /locus_tag="KW011_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HBH7658731.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26714..28087
                     /gene="cobA"
                     /locus_tag="KW011_000289"
     CDS             26714..28087
                     /gene="cobA"
                     /locus_tag="KW011_000289"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBH7658732.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28334..28501
                     /locus_tag="KW011_000290"
     CDS             28334..28501
                     /locus_tag="KW011_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HBH7658733.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28745..30133
                     /gene="frlA"
                     /locus_tag="KW011_000291"
     CDS             28745..30133
                     /gene="frlA"
                     /locus_tag="KW011_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HBH7658734.1"
                     /translation="MTANSPLQRIGQEKGIAMGSQELQRKLGFWAVLAIAVGTTVGSG
                     IFVSVGEVAKAAGTPWLTVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGS
                     RPLAFLSGWASFWANDAPSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHL
                     RSVEGGAAFQTLITIAKIIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGI
                     SATSWSYTGMASICYMTGEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKL
                     ANSETPISDALTWIPALGSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFF
                     KCFGHVHPKYNTPDVSIILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWC
                     RKRDDYKPLWRTPAFGLMTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAF
                     WAKRSRQLNALS"
     gene            30154..31176
                     /gene="frlB"
                     /locus_tag="KW011_000292"
     CDS             30154..31176
                     /gene="frlB"
                     /locus_tag="KW011_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HBH7658735.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKVKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31226..32057
                     /gene="frlC"
                     /locus_tag="KW011_000293"
                     /pseudo
     CDS             31226..32057
                     /gene="frlC"
                     /locus_tag="KW011_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            32054..32839
                     /gene="frlD"
                     /locus_tag="KW011_000294"
     CDS             32054..32839
                     /gene="frlD"
                     /locus_tag="KW011_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HBH7658736.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32939..33670
                     /gene="phoB"
                     /locus_tag="KW011_000295"
     CDS             32939..33670
                     /gene="phoB"
                     /locus_tag="KW011_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HBH7658737.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33822..34907)
                     /locus_tag="KW011_000296"
     CDS             complement(33822..34907)
                     /locus_tag="KW011_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH7658738.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(34919..36223)
                     /locus_tag="KW011_000297"
     CDS             complement(34919..36223)
                     /locus_tag="KW011_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HBH7658739.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36235..36588)
                     /locus_tag="KW011_000298"
     CDS             complement(36235..36588)
                     /locus_tag="KW011_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HBH7658740.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36599..37477)
                     /locus_tag="KW011_000299"
     CDS             complement(36599..37477)
                     /locus_tag="KW011_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HBH7658741.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(37474..38700)
                     /locus_tag="KW011_000300"
                     /pseudo
     CDS             complement(37474..38700)
                     /locus_tag="KW011_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            complement(38700..39863)
                     /locus_tag="KW011_000301"
                     /pseudo
     CDS             complement(38700..39863)
                     /locus_tag="KW011_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            complement(39947..40309)
                     /locus_tag="KW011_000302"
     CDS             complement(39947..40309)
                     /locus_tag="KW011_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH7658742.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(40326..41231)
                     /locus_tag="KW011_000303"
     CDS             complement(40326..41231)
                     /locus_tag="KW011_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7658743.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41391..42395)
                     /gene="trpS"
                     /locus_tag="KW011_000304"
     CDS             complement(41391..42395)
                     /gene="trpS"
                     /locus_tag="KW011_000304"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBH7658744.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42388..43146)
                     /gene="gph"
                     /locus_tag="KW011_000305"
     CDS             complement(42388..43146)
                     /gene="gph"
                     /locus_tag="KW011_000305"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HBH7658745.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43139..43816)
                     /gene="rpe"
                     /locus_tag="KW011_000306"
     CDS             complement(43139..43816)
                     /gene="rpe"
                     /locus_tag="KW011_000306"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBH7658746.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43834..44670)
                     /gene="dam"
                     /locus_tag="KW011_000307"
     CDS             complement(43834..44670)
                     /gene="dam"
                     /locus_tag="KW011_000307"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBH7658747.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44777..46063)
                     /gene="damX"
                     /locus_tag="KW011_000308"
     CDS             complement(44777..46063)
                     /gene="damX"
                     /locus_tag="KW011_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HBH7658748.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46155..47243)
                     /gene="aroB"
                     /locus_tag="KW011_000309"
     CDS             complement(46155..47243)
                     /gene="aroB"
                     /locus_tag="KW011_000309"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBH7658749.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47300..47821)
                     /gene="aroK"
                     /locus_tag="KW011_000310"
     CDS             complement(47300..47821)
                     /gene="aroK"
                     /locus_tag="KW011_000310"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HBH7658750.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48222..49460)
                     /gene="hofQ"
                     /locus_tag="KW011_000311"
     CDS             complement(48222..49460)
                     /gene="hofQ"
                     /locus_tag="KW011_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HBH7658751.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49372..49776)
                     /gene="hofP"
                     /locus_tag="KW011_000312"
     CDS             complement(49372..49776)
                     /gene="hofP"
                     /locus_tag="KW011_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HBH7658752.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(49766..50206)
                     /gene="hofO"
                     /locus_tag="KW011_000313"
     CDS             complement(49766..50206)
                     /gene="hofO"
                     /locus_tag="KW011_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HBH7658753.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(50190..50729)
                     /gene="hofN"
                     /locus_tag="KW011_000314"
     CDS             complement(50190..50729)
                     /gene="hofN"
                     /locus_tag="KW011_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HBH7658754.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTD
                     NAEARVNTVLLQAEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50729..51508)
                     /gene="hofM"
                     /locus_tag="KW011_000315"
     CDS             complement(50729..51508)
                     /gene="hofM"
                     /locus_tag="KW011_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HBH7658755.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51628..54180
                     /gene="mrcA"
                     /locus_tag="KW011_000316"
     CDS             51628..54180
                     /gene="mrcA"
                     /locus_tag="KW011_000316"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HBH7658756.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54345..54905)
                     /gene="nudE"
                     /locus_tag="KW011_000317"
     CDS             complement(54345..54905)
                     /gene="nudE"
                     /locus_tag="KW011_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HBH7658757.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55225..57360
                     /gene="igaA"
                     /locus_tag="KW011_000318"
     CDS             55225..57360
                     /gene="igaA"
                     /locus_tag="KW011_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HBH7658758.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            57425..58093
                     /gene="yrfG"
                     /locus_tag="KW011_000319"
     CDS             57425..58093
                     /gene="yrfG"
                     /locus_tag="KW011_000319"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HBH7658759.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58104..58505
                     /gene="hslR"
                     /locus_tag="KW011_000320"
     CDS             58104..58505
                     /gene="hslR"
                     /locus_tag="KW011_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HBH7658760.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58530..59408
                     /gene="hslO"
                     /locus_tag="KW011_000321"
     CDS             58530..59408
                     /gene="hslO"
                     /locus_tag="KW011_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBH7658761.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(59543..60916)
                     /locus_tag="KW011_000322"
     CDS             complement(59543..60916)
                     /locus_tag="KW011_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HBH7658762.1"
                     /translation="MAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNG
                     LTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALVVILARTQSRLIDSIQ
                     KLFTLIATGLLPLVSLLTLMFIITLPFTDLSAISRHISAAGLLLTLAFLQLILMAIVR
                     DPQKASLPWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVL
                     LVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMA
                     RYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQ
                     EQVSEKVLAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQA
                     ERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLDLTVDGET
                     LEINLSK"
     gene            complement(60929..61243)
                     /locus_tag="KW011_000323"
     CDS             complement(60929..61243)
                     /locus_tag="KW011_000323"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBH7658763.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FG"
     gene            61622..63244
                     /gene="pckA"
                     /locus_tag="KW011_000324"
     CDS             61622..63244
                     /gene="pckA"
                     /locus_tag="KW011_000324"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBH7658764.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKLDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63320..64672)
                     /gene="envZ"
                     /locus_tag="KW011_000325"
     CDS             complement(63320..64672)
                     /gene="envZ"
                     /locus_tag="KW011_000325"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HBH7658765.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64669..65388)
                     /gene="ompR"
                     /locus_tag="KW011_000326"
     CDS             complement(64669..65388)
                     /gene="ompR"
                     /locus_tag="KW011_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HBH7658766.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65616..66092
                     /gene="greB"
                     /locus_tag="KW011_000327"
     CDS             65616..66092
                     /gene="greB"
                     /locus_tag="KW011_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HBH7658767.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            66189..68510
                     /locus_tag="KW011_000328"
     CDS             66189..68510
                     /locus_tag="KW011_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBH7658768.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            68948..69175
                     /gene="feoA"
                     /locus_tag="KW011_000329"
     CDS             68948..69175
                     /gene="feoA"
                     /locus_tag="KW011_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HBH7658769.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69192..71513
                     /gene="feoB"
                     /locus_tag="KW011_000330"
     CDS             69192..71513
                     /gene="feoB"
                     /locus_tag="KW011_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HBH7658770.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71513..71749
                     /gene="feoC"
                     /locus_tag="KW011_000331"
     CDS             71513..71749
                     /gene="feoC"
                     /locus_tag="KW011_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HBH7658771.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            71952..>72510
                     /locus_tag="KW011_000332"
     CDS             71952..>72510
                     /locus_tag="KW011_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBH7658772.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQR"
BASE COUNT        17432 a        19699 c        18349 g        17030 t
ORIGIN      
        1 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      121 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      181 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      241 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      301 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      361 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      421 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      481 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      541 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      601 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
      661 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
      721 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
      781 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
      841 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
      901 atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc
      961 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1021 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1081 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1141 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1201 cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc
     1261 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1321 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1381 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1441 cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc
     1501 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1561 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1621 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     1681 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     1741 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     1801 tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc
     1861 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     1921 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac
     1981 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2041 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2101 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2161 ctcatactgc ttagccatac cagaccagaa tgcagtagtc gcagcggaag tgatggtaat
     2221 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2281 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2341 gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat
     2401 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2461 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2521 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2581 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     2641 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     2701 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     2761 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     2821 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     2881 gatatttaca aatttagcca gcagttctga tccgaacttc ggatccggca gaattttacg
     2941 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3001 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3061 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3121 gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3181 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3241 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3301 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3361 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3421 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3481 taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga aaagatgtca
     3541 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3601 ctgacgaaat cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg
     3661 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     3721 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtcg
     3781 ccttcactga gcagacgcag cagcgcagca aaatccgcca gccagggtga acgatgtaat
     3841 gtgtgcagca taaaagcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     3901 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggggtctgcg gatctaaccc
     3961 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa
     4021 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctggcagcag
     4081 ctgaaaaacg ccatcagcta taaagaagac agccagatca tcagttaatg cggaagttgc
     4141 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggcgcag tagaaaaaac
     4201 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4261 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaggc cagccccagc
     4321 cttccggcct ccgtttcatc aacaacgcca cggcgtaatg ctgccgctac gcagatattc
     4381 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg
     4441 cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgataaaa aaagacgctg
     4501 cttaactcat ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4561 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4621 ggaaatcacc gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     4681 ggcggtcagc aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat
     4741 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca tttacttctt
     4801 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     4861 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     4921 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     4981 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5041 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5101 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5161 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5221 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5281 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5341 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5401 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5461 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5521 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5581 tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     5641 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     5701 caccccggga taacaccgtc cagacggaaa gagagcggtt caccacgggt gtaagagttg
     5761 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     5821 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     5881 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     5941 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6001 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6061 ccagcgatca gctgatcttt atccagtttg atgcccagtt tttcttgttc tttaagagag
     6121 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6181 ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6241 attggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat
     6301 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6361 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6421 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6481 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6541 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6601 gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     6661 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     6721 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     6781 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     6841 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     6901 atcgtggtga tgatcgtgcg cgccgtgaac gtgaccatga gccagttctt cttcggtcgc
     6961 ttcacgaatc gccacaacct caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7021 accatcaacc actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7081 gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7141 tttaggaaca cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7201 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7261 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7321 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7381 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7441 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7501 tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7561 gtccgcactt aacgcattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7621 cctgtgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct
     7681 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     7741 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacagtat
     7801 cggcatgcat tgggatgagc tctcgcagca tccgcatatc caggcggcga aaatgcagtt
     7861 gcgcccgctg cgcctgatgc ggatgcatcc caagcgtgac caaagtcttg cgccccagct
     7921 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     7981 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8041 atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag
     8101 cggcctctgc acccgcagaa cgtaatagat cgacctgcgt ggcgtcgccg taataaactt
     8161 tgtagccata tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc
     8221 gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga
     8281 caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt
     8341 taaactggcg ggatagccat ttatccacca gcttcatcag caacggcgtg gtcatcatgg
     8401 aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ctcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ccgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccagcgt taccgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc agggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcagg cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gagacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtggtatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc ccccgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat
    13321 agcctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga ggctaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg gacattcacc ccctggcaac ctttaccgga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtggtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctc gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcagc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atagaaacca catcgatggc actgcaacca cccgccgcca
    14581 tcaacaccat ttccatcgga cttggtgctt tatcgcctga gttgccgtcc attaaaatct
    14641 ggtgaccaga ggcggattcg cccagaaaag ttaacccttc gacccacttc actcgcgctt
    14701 gcatttttgc tactccactg cgtcaatttt cctgacagag tacgcgtact aaccaaatcg
    14761 cgcaacggaa ggcaacctgg gtcatgctga agcgagacac caggagacac aaagcgaaag
    14821 ctatgctaaa acagtcagga tgctacagta atacattgat gtactgcatg tatgcaaagg
    14881 acgtcacatt accgtgcagt acagttgata gccccttccc aggtagcggg aagcatattt
    14941 cggcaatcca gagacagcgg cgttatctgg ctctggagaa agcttataac agaggataac
    15001 cgcgcatggt gcttggcaaa ccgcaaacag acccgactct cgaatggttc ttgtctcatt
    15061 gccacattca taagtaccca tccaagagca cgcttattca ccagggtgaa aaagcggaaa
    15121 cgctgtacta catcgttaaa ggctctgtgg cagtgctgat caaagacgaa gagggtaaag
    15181 aaatgatcct ctcctatctg aatcagggtg attttattgg cgaactgggc ctgtttgaag
    15241 agggccagga acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt
    15301 cgtacaaaaa atttcgccaa ttgattcagg taaacccgga cattctgatg cgtctgtctg
    15361 cacagatggc gcgtcgtctg caagtcactt cagagaaagt gggcaacctg gcgttcctcg
    15421 acgtgacggg ccgcattgca cagactctgc tgaatctggc aaaacaacca gacgctatga
    15481 ctcacccgga cggtatgcaa atcaaaatta cccgtcagga aatcggtcag attgtcggct
    15541 gttctcgtga aaccgtggga cgcattctga agatgctgga agatcagaac ctgatctccg
    15601 cacacggtaa aaccatcgtc gtttacggct ctcgttaatc ccgtcggagt ggcgcgttac
    15661 ctggtagcgc gccattttgt ttcccccgat gtggcgcaga ctgatttatc accccgatat
    15721 caactatgca cttcgacaaa cgctggtgct atgtttgccc gtggccgttg ggttaatgct
    15781 tggcgaatta cgattcggtc tgctcttctc cctcgttcct gcctgttgca atattgcggg
    15841 ccttgatacg cctcataaac gttttttcaa acgcttaatc attggtgcgt cgctgtttgc
    15901 cacctgtagc ttgctgacac agctactact ggcaaaagat gttcccctgc cctttttgct
    15961 gaccggatta acgctggtac ttggcgtcac tgctgagctg gggccattgc acgcaaaatt
    16021 gcttcctgca tcgctgctcg ccgccatttt taccctcagt ctggcgggat acatgccggt
    16081 ctgggaaccg ttgctcatct atgcgttggg cactctctgg tacggattgt ttaactggtt
    16141 ttggttctgg atctggcgcg aacaaccgct gcgcgagtca ctaagtctgc tgtaccgcga
    16201 actggcagat tattgtgaag ccaaatacag cctgcttacc cagcacaccg accctgaaaa
    16261 agcgctgccg ccgctgctgg tgcgccagca aaaagcggtc gatctaatta cccagtgcta
    16321 tcagcaaatg catatgcttt ccgcgcaaaa taatactgat tacaagcgga tgctgcgtat
    16381 tttccaggag gcgctggatt tacaggaaca tatttcggtc agtttgcatc agccggaaga
    16441 ggtgcaaaag ctggtcgagc gtagccatgc ggaagaagtt atccgctgga atgcgcaaac
    16501 cgtcgccgct cgcctgcgcg tgctggctga tgacattctt taccatcgcc tgccaacgcg
    16561 ttttacgatg gaaaagcaaa ttggcgcact ggaaaaaatc gcccgccagc atccggataa
    16621 tccggttggg caattctgct actggcattt cagccgcatc gcccgcgtgc tgcgcaccca
    16681 aaaaccgctc tatgcccgtg acttactggc cgataaacag cggcgaatgc cgttacttcc
    16741 ggcgctgaaa agttatctgt cactaaagtc tccggcgcta cgcaatgccg gacgactcag
    16801 tgtgatgtta agcgttgcca gcctgatggg caccgcgctg catctgccga agtcgtactg
    16861 gatcctgatg acggtattgc tggtgacaca aaatggctat ggcgcaaccc gtctgaggat
    16921 tgtgaatcgc tccgtgggaa ccgtggtcgg gttaatcatt gcgggcgtgg cgctgcactt
    16981 taaaattccc gaaggttaca ccctgacgtt gatgctgatt accaccctcg ccagctacct
    17041 gatattgcgc aaaaactacg gctgggcgac ggtcggtttt actattaccg cagtgtatac
    17101 cctgcaacta ttgtggttga acggcgagca atacatcctt ccgcgtctta tcgataccat
    17161 tattggttgt ttaattgctt tcggcggtac tgtctggctg tggccgcagt ggcagagcgg
    17221 gttattgcgt aaaaacgccc atgacgcttt agaagcctat caggaagcga ttcgcttgat
    17281 tcttagcgag gatccgcaac ctacgccact ggcctggcag cgaatgcggg taaatcaggc
    17341 acataacact ctgtataact cattgaatca ggcgatgcag gaaccggcgt ttaacagcca
    17401 ttatctggca gatatgaaac tgtgggtaac gcacagccag tttattgttg agcatattaa
    17461 tgccatgacc acgctggcgc gggaacaccg ggcattgcca cctgaactgg cacaagagta
    17521 tttacagtct tgtgaaatcg ccattcagcg ttgtcagcag cgactggaat atgacgaacc
    17581 ggggagttct ggcgatgcca atatcatgga ttcgccggag atgcagccgc acgaaggcgc
    17641 ggcaggtacg ctggagcagc atttacagcg ggttattggt catctgaaca ccatgcacac
    17701 catttcgtcg atggcatggc gtcagcgacc gcatcacggg atttggctga gtcgcaagtt
    17761 gcgggattcg aaggcgtaat gcaggctaaa ccatcggata aggcattcac gccgcatccg
    17821 acatcttttg cctgatgctt cgtatttacg ccccaaccac cttcgccacc gcgtgggcga
    17881 aacgttgcat cccttcatcg atatccgcat cttccaccac cagcgacggt gcaaaacgca
    17941 tcacatccgg tccggcattc agcaccatta cgccagcctc tgcgcccgca tacaggaaat
    18001 cacgcgcccg acctttgtac tgtggtttca gctctgcgcc aatcaacagc cccataccgc
    18061 gaatatcgct aaacacatcg tactgctgat cgatcttctg cagatggtca acaaaacgct
    18121 ggcgtttcgc ctgaatgcct tccagcactt cagggggatt gatgatatca aacgccgccc
    18181 ccgctactgc acaggccaga ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa
    18241 acgcagaagc aatttccgcc gtggtcagca tggcgctaat cgggaagccg ccgcctaacg
    18301 ctttcgcaga ggtcagaata tccggcgtca cgccgtagtg catgtaagca aacaaatcgc
    18361 cggtccgccc catcccgcac tgcacttcat caaacaccaa taatgcctga tgttgatcgc
    18421 acagctcgcg caagccctgc aaaaactctg gcgtcgcagc cgtcacgccg ccctcgccct
    18481 ggatcggctc aaccaccacc gcacaggtgt gatcatccat cactgctttc actgcgtgga
    18541 gatcgttaaa gggaacgtgg atgatgtctg ccggtttcgg cccaaagccg tcggaatatt
    18601 ttggctgccc acccaccgaa acggtaaaca gcgagcgacc atgaaaagcg ttatggaagg
    18661 caataatttt ggttttgaac gggctatgac gcacacaggc gtaatgacgt gccagtttaa
    18721 aggcggtttc gttagcttcc gtgccggagt tcataaacac cacgcgttcg gcaaacgttg
    18781 cctcaatcag tttacgccca agacgcagcg ccggttcatt ggtgaaaacg ttactgatat
    18841 gccacagaat ttcgccctgg gtttttaacg cgttcaccag cgcaggatgg caatggccca
    18901 acgccgtaac tgcaatgcca cccgcgaaat cgacatactc cttgccttgc tgatcccaga
    18961 ttcggctgcc ctgacctttt accggaataa actctgccgg agcataaatc ggcaggatca
    19021 cttcatcgaa agtcgcgcgt gtaattgctg tttgttcaat tgccatctca tgatcaccct
    19081 gttacgcata aacaaatgtg aaattataat cacaaaatat gcataaaaaa tcactaaatg
    19141 gcaatcagaa atcagcgatg caggaaatta gccagcagtt gatgtccttg ttcgctaaga
    19201 atactttctg gatggaactg cacaccttcc agatcccact ggcgatggcg aatccccata
    19261 atctcgcggg tttcgctcca ggccgtcacg tcaaagcacg ctggtaatga gtcaggttcc
    19321 accaccagcg aatggtagcg tgtcacggta agtggatttg ccagcccccg aaatacgccc
    19381 tcaccgctat gtgtaatcgg cgaggttttg ccgtgcatga cctttgcggc gcgcacaact
    19441 ttgccgccaa atgcctgcgc cattgcctga tgaccgaggc agacgccaag aatcggcaag
    19501 cgcccggcat agtgacgaat aacgtcaagg gagatcccgg cttcatctgg cgtacagggg
    19561 ccaggtgaga tgacaatttt ttgtggttta agggcgtcga tatccgccag cgtcaacgca
    19621 tcgttgcgct taaccagcac atccgccccc agttcacaaa agtactggta gaggttccag
    19681 gtaaaagaat cgtagttatc tataagcagg atcatggcgg ctccggtacg aaagaaccgc
    19741 gctattttac tcagattccc cggctttgct taccactttg ctaaatatca tctgcagtgc
    19801 ggtgagatcc cccattgccc cactctgatt tgcctgattc caggcctctt tttcgatacc
    19861 ctgccagctc agttgatatc cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc
    19921 cagaccactt cccacccgga agggatgcag cacgttgatt tcacaatagt aatgcgccag
    19981 ccgctcgacg aacttcgctt tctccaggcc aaccagataa ccttcttcct ccagatcctg
    20041 catcagggca ttgccctctt tttcgatata agcaaagtgg cagaacggcg tatcgccctg
    20101 ataaatatca acttcacgca gttgccctgc ccagtcgaaa atatcctggt acagctggcg
    20161 atggatagtg cgcaaatgcg gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc
    20221 acgcagcgcc gtcatttcgt aagcggcctg ttccagccgc tgctgctgac ggatgttcag
    20281 ccggttacgc atgatatcaa ggccaggata aagatacgga tcgcgccctt cgccgaattt
    20341 atcgctcata gtgcctcctc agctcttcaa ggcgcgctaa agcctctgcg gcagtaagag
    20401 tgactaaagg catctcgacg ccttcaagac ggcgactggc ctggaaatta cgattacgct
    20461 gaagctccca gagacgggac ttttgcttat cggtgagttt cttcacttga tgcctccctg
    20521 aacgtgtcca tttgccacaa gtataagcgg caaatccggg ttacgccggg agagcagaag
    20581 cgcgggcaag attgcccgcg cgagaaatca ttacggcagg actttagcgg aaaggataac
    20641 taccggtttt gacggcacat tctggtacgg accaacatca tgagtcggca cctgggaaat
    20701 cttatcggca acgtccatgc ctttcaccac tttaccaaat accgcgtaac cgaaatcacg
    20761 ctgaccatgg tcaaggaagg cgttatcggc aacgttgata aagaactggc tggtggcgct
    20821 gtctttgtca gcggtacgcg ccatcgcgat ggtgccacgc gtgttgcgca ggccgttatc
    20881 ggcttcattt ttgattggcg ggtttggttt tttctgctgc atctgctcgg tgaaaccgcc
    20941 gccctgaatc ataaagccag gaatgacgcg gtgaaaggta gtgttgttat aaaaaccgct
    21001 gttcacataa tcgacaaagt tttgcacaga cactggcgct ttttgtttat ccagctccag
    21061 ttcgatgtta ccagctgagg ttgtcaacaa tacgtgcggg tcccctttcg ctgccattgc
    21121 tgcgggagaa agagcagaaa gagcgaaaac agcagccatc gccgccaggg tcgatttgaa
    21181 catgagattt ccttaacaaa cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc
    21241 ttcaagaaca aggccatccc tttacctaat tttacgtatc tgaaatatat gtaacctgac
    21301 gatcactttt tattccgtga tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc
    21361 aataaaacat ttaaacagat cacaaaatca cctaaaatcg cccgctcgcg gcatatcccg
    21421 ctatgccatt ttatttttga acgcaggcca atcatgacta acagcaatcg catcaagctc
    21481 acatggatta gctttctctc ctacgcactg actggtgcgt tggttattgt caccgggatg
    21541 gtgatgggaa atatcgccga ttatttcaat ctgcctgttt ccagtatgag taacaccttc
    21601 accttcctca acgccggcat tttaatctct atcttcctca acgcctggct gatggaaatc
    21661 gtcccgttga aaacgcagtt acgttttggc tttctcctga tggtgctggc ggttgccggt
    21721 ttgatgttca gccacagcct ggcgctgttc tcgacggcga tgttcattct cggggtggtc
    21781 agcggcatta ctatgtcgat tggtacattc ctgataacac aaatgtatga agggcgtcag
    21841 cgcggttccc gcctgttatt tactgactcc ttcttcagta tggctgggat gattttccca
    21901 atgatcgccg cgtttctact ggcgcgcagc attgagtggt actgggttta tgcctgcatc
    21961 gggctggtgt atgtcgctat ttttattctg accttcggct gtgagttccc ggcactgggc
    22021 aaacatgcac caaaaacgga tgctccggta gagaaagaaa agtgggggat cggcgtactg
    22081 tttctctctg ttgcggcgct gtgctacatc ctcggtcagt taggttttat ctcctgggtg
    22141 cctgagtatg ccaaaggcct gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac
    22201 ttctggatgt catacatggt cggcatgtgg gcgttcagct ttattcttcg cttctttgat
    22261 ttgcaacgca ttctgaccgt actggctggt ctggctgcga ttctgatgta cgtctttaac
    22321 accggaacac cagcacatat ggcgtggtca attctcgctc tgggcttctt ctccagcgcg
    22381 atctatacca ccatcatcac cctgggttca cagcagacca aagtaccgtc gccaaaactg
    22441 gttaactttg tcctgacctg cggaaccatc ggtactatgt tgacctttgt ggttaccggc
    22501 ccgattgttg aacatagcgg tccgcaggcg gcactgctta cagcaaacgg tctgtacgct
    22561 gtcgtctttg tgatgtgctt cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc
    22621 ctgacctctc attaattgct aatgccggac ggcactatcg tcgtccggcc ttttcctctc
    22681 ttcccccgct acgtgcatct atttctataa acccgttcat tttgtccatt ttttgcacaa
    22741 acatgaaatg tcagacaatt ccgtgactta agaaaattta tacaaatcag caacatacca
    22801 cttaaggagt atataaaggt gaatttgatt tacatcaata agcggggttg ctgaatcgtt
    22861 aaggtaggcg gtaatagaaa agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt
    22921 atcggtaacg gtatggtcgg ccatcgcttt atcgaagatc ttcttgataa atctgatgcg
    22981 gccaactttg atattaccgt tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac
    23041 ctctcgtctt acttctctca ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc
    23101 tacgagaaac acggcatcaa agttctggtc ggcgaacgcg ctatcaccat caaccgtcag
    23161 gagaaggtga ttcactccag cgccggacgt accgtttttt atgacaagct gatcatggca
    23221 accggttcct acccgtggat cccaccaatc aaaggttctg atactcagga ctgctttgtc
    23281 tatcgcacca ttgaagacct caacgccatt gaatcctgcg cccgtcgcag caaacgcggt
    23341 gccgttgttg gtggcggcct gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt
    23401 attgaaaccc acgttatcga atttgcccct atgctgatgg cggaacagct tgatcagatg
    23461 ggtggcgagc agctgcgtcg caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa
    23521 aacacccttg agattgtgca ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac
    23581 ggcagcgaac tggaagtcga ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag
    23641 ctggcaaccc agtgtggtct ggacgttgct ccgcgtgggg gtatcgtcat taatgattcc
    23701 tgccagactt ccgatccgga tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt
    23761 gtatttggtc tggtagcacc tggctacaaa atggcgcagg tcgccgttga ccatattctc
    23821 ggtagcgaaa acgcctttga aggtgctgac cttagcgcca agctgaaact gctgggcgta
    23881 gacgtaggcg gtattggtga tgcgcacggt cgcacgcctg gcgcacgtag ctacgtttac
    23941 ctcgacgaaa gtaaagagat ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg
    24001 ctcggtgcgg tactggtggg cgataccagc gactacggta acctgctgca actggtgctg
    24061 aacgctatcg aactgccgga aaacccggat tccctgatcc tgccagcaca ctctggtagc
    24121 ggcaagccgt ctatcggtgt tgataaactg ccggacagcg cgcaaatctg ctcctgcttc
    24181 gacgtcacta aaggtgatct gattgctgcc atcaacaaag gctgccacac agttgcggcg
    24241 ctgaaagctg aaaccaaagc gggtactggc tgcggtggct gtatcccgct ggtcactcag
    24301 gtactgaacg cggaactggc gaaacagggc atcgaagtta acaacaacct gtgcgaacac
    24361 tttgcttatt cgcgtcagga actgttccat ttgatccgcg ttgaaggcat taaaaccttc
    24421 gaagaactgc tggcgaaaca cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc
    24481 ggttcgctgc tggcctcctg ctggaacgaa tacattctga agccggaaca tactccgctg
    24541 caggattcta acgacaactt cctcgctaac atccagaaag acggcaccta ctcggtgatc
    24601 ccgcgttctc cgggcggtga aatcaccccg gaagggctga tggcagtggg tcgtatcgcg
    24661 cgtgaattta atctctacac caaaatcacc ggctcccagc gcctggcgat gtttggcgca
    24721 cagaaagacg atctgccgga aatctggcgt cagctgattg aagctggctt cgaaaccggt
    24781 catgcctatg cgaaagcact gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc
    24841 tacggtgttg gcgatagcgt cggcctcggc gtggaactgg aaaaccgcta caaaggcatc
    24901 cgtacgccgc acaaaatgaa gttcggtgtc tccggctgta cccgtgaatg ttcagaagct
    24961 cagggtaaag acgtgggtat tatcgccact gaaaaaggct ggaacctgta tgtttgcggt
    25021 aacggcggca tgaaaccgcg tcatgcggat ctgctggcag cggatatcga tcgcgaaacg
    25081 ctgatcaaat atctcgaccg cttcatgatg ttctacatcc gtaccgccga caaactgacg
    25141 cgtaccgcgc cgtggttaga aaacctcgaa ggcggcatcg attacctgaa agcggtgatc
    25201 attgacgaca aactggggct gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa
    25261 gcggtagtgt gtgagtggac tgaaacggtc aatacgccgt ctgcgcagac tcgcttcaaa
    25321 cacttcatca acagcgacaa gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag
    25381 caccgtccgg caacgccgta tgaacgtatc ccggtaactc tggtggagga caacgcatga
    25441 gccagtggaa agacatctgc aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc
    25501 tgttaggtga cgagcaggta gcgattttcc gcccgtatca cagcgaccag gtgtttgcga
    25561 tcagcaacat tgatccgttc ttcgagtcca gcgtgctatc acgcgggctg attgcggaac
    25621 accagggcga gctgtgggtc gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg
    25681 gcttgtgcat ggaagacgaa cagttttccg tcaaacatta cgaagcgcga gtgaaagacg
    25741 gcgtggtgca gctgcgcggt taatgtttta acgggaggcg caatgcctcc cctttttgca
    25801 tggtcctgta atgaccttcg gtatattgca gaacattttt taaacttttt gttttatttt
    25861 ttgtttttat tttttaaagg ataatcaaat gtttacagac actattaata agtgtgcggc
    25921 taatgctgcg cgcattgcac gcctgtcggc aaataacccg ctcggctttt gggtcagctc
    25981 cgccatggcg ggcgcatatg tgggtcttgg gatcatcctg attttcacgc tcggtaattt
    26041 gctcgacccg tccgtacgtc cgctggtgat gggcgcgacc tttggtatcg ccttaacgct
    26101 ggtgattatc gccggttctg aactgttcac cggacacgcc atgttcctca cctttggggt
    26161 aaaagcgggc agcatcagcc acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg
    26221 taacctggtc ggttccgtct tcgttgccat gctctatagc tggggcggcg gtagcctgct
    26281 gccggtagat accagcatcg ttcactccgt cgcgctggct aaaaccactg caccggcaat
    26341 ggtactgttc ttcaaaggtg cattgtgtaa ctggctggtt tgcctggcta tctggatggc
    26401 gctgcgcact gaaggggcgg cgaaatttat cgctatctgg tggtgtctgc tggcatttat
    26461 cgcgtccggc tacgagcact ccatcgctaa catgacgctg ttcgccctct cctggttcgg
    26521 caaccacagc gaagcctaca cgctggcggg tattggtcat aacctgctgt gggtgacgct
    26581 gggtaatact ttatcgggtg ccgtattcat gggattgggt tattggtatg ctacgccgaa
    26641 agcgaatcgt ccggttgcgg acaaatttaa tcaaactgaa acggctgccg gttaattact
    26701 aaggggtttt tacgtggatc atttgcctat attttgccaa ttacgcgatc gcgactgtct
    26761 gattgtcggc ggtggtgatg tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc
    26821 tcgcttaacg gtgaatgcat tagcgtttat tccacagttc accgcatggg cagatgcagg
    26881 catgttaacc ctcgtcgaag ggccatttga tgaaagcctt ctcgacacct gctggctggc
    26941 gattgcagcg acggatgatg acacgcttaa ccagcgcgtc agcgaagccg ctgaagctcg
    27001 tcgcatcttc tgtaacgtag tcgatgcgcc gaaagccgcc agctttatta tgccgtcgat
    27061 tattgaccgc tcaccgctaa tggtagcggt ctcctctggc ggcacctctc cggttctggc
    27121 gcgcctgttg cgcgaaaaac tcgaatcact gctgccgttg catctgggcc aggtggcgaa
    27181 atatgccggg caattacgcg ggcgagtgaa acaacagttc gccacgatga gtgagcgtcg
    27241 ccgtttctgg gagaaattgt tcgttaacga ccgcctggcg cagtcgctgg caaacaacga
    27301 tcagaaagcc attactgaaa cgaccgaaca gttaatcaac gaaccgctcg accatcgcgg
    27361 tgaagtggtg ctggttggtg caggtccggg cgatgccggg ctgctgacgc tgaaaggact
    27421 gcaacaaatt cagcaggcag atgtggtggt ctacgaccgt ctggtttctg acgatattat
    27481 gaatctggta cgccgcgatg ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg
    27541 tgtaccgcag gaagagatta accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt
    27601 ggtgcggctg aaaggtggcg atccgtttat ttttggccgt ggtggcgaag agctggaaac
    27661 actgtgcaac gcgggtattc cgttctcggt ggttccgggt attaccgcag cttctggttg
    27721 ctctgcctat tcgggtattc cactcacgca tcgcgattat gcccagagcg tacgcttaat
    27781 taccggacac ttaaaaaccg gtggcgagct ggactgggaa aacctggcgg cagaaaaaca
    27841 gacgctggtg ttctatatgg gattgaatca ggccgcgact attcagcaaa agctgattga
    27901 acacggtatg cctggcgaaa tgccggtggc aattgtcgaa aacggaacgg cagtcacgca
    27961 gcgcgtgatt gacggtacgc tcacgcaact cggtgaactt gctcagcaaa tgaacagtcc
    28021 atcgctaatt attattggtc gggttgttgg cctgcgcgat aaactgaact ggttctccaa
    28081 ccattaattt aacccgggcc agagaattct ggccttctta atgactcttt ttatatattc
    28141 agcaaataaa acataacccc ttaataaata ttatctgcta atgggttata ttcaccgtta
    28201 cagcgactta caaaattaag tcattcgctc actgacgcca attaaatatt cctgtatcgt
    28261 tcttaacgag cagtctgctt atattcatat aatcaatgaa tattaattaa tatataatac
    28321 atagggaatg taaatgaaca aatttattaa agttgcactg gtaggtgcag tactggctac
    28381 gttaactgca tgtactggtc atattgaaaa ccgtgataag aactgctctt acgactacct
    28441 gctgcacccg gcaatttcta tttctaaaat cattggcggt tgcggtccta ctgcacagta
    28501 agtctctgac agaaatcggc taacaccggt ttaattgccc gacgccctct ggattttccg
    28561 agggcgtatt tgtttctatg tccttcttgc ccccgcccgt aaaaatattt tcactttaaa
    28621 ttcaatttga taactacatc cattagccac agcgcgatcc tgtgcgaaat tttgtgatct
    28681 tcctccacat tacataacat catatgttgt tatattcatc atgcattgtc atgttacctt
    28741 ttaaatgact gcaaactctc ccctacaacg tattggacaa gaaaaaggta tcgctatggg
    28801 aagccaggaa ctccaacgca agctcggatt ttgggccgtt cttgcaatcg ccgtcgggac
    28861 aaccgtcggc tccggtattt ttgtatctgt gggtgaagtg gcaaaagcag cgggcacgcc
    28921 gtggcttacg gtgctcgcgt ttgtcattgg cgggttaatt gtgatcccgc aaatgtgcgt
    28981 ctatgcggaa ctatccaccg cttatccgga aaatggcgca gattatgttt atctgaaaaa
    29041 tgccggaagc cgaccgctgg ctttcctctc cggctgggcc agcttctggg ccaacgatgc
    29101 accgtcattg tcgattatgg cgctggcgat tgtcagcaat cttggctttt taacgcctat
    29161 cgatccgttg ctcggtaaat ttatcgccgc cggattaatt atcgccttta tgttgctaca
    29221 cctgcgctcc gttgaaggcg gcgcagcgtt tcagacgcta attaccatcg ccaaaattat
    29281 cccgttcact atcgtcattg gccttgggat cttctggttt aaagcggaga attttgccgc
    29341 ccctaccacc actgcgattg gcgcaacggg cagctttatg gcgctgctgg cggggatctc
    29401 tgccaccagt tggtcgtata ccggcatggc ctctatctgt tatatgaccg gcgaaattaa
    29461 aaaccccgga aaaaccatgc cacgagcgct gattggttcc tgtctgctgg ttctggtgct
    29521 ctacaccctg ctggcgctgg tgatttccgg cctgatgccc ttcgacaaac tcgccaattc
    29581 tgaaacgccg atttccgacg ccctgacctg gatccccgca ctcggcagca ccgctgggat
    29641 ctttgttgcc atcacggcga tgatcgtcat tcttggttcg ctttccagct gcgtgatgta
    29701 ccagccgcgg ctggaatacg cgatggcgaa agacaacctg ttctttaaat gcttcggcca
    29761 tgtgcatccg aaatacaaca cgccggatgt ctccatcatc ctgcaagggg cgctggggat
    29821 cttcttcatc ttcgtttccg atctcaccag cctgctgggt tatttcaccc tggtgatgtg
    29881 tttcaaaaat accctcacct tcggctccat catctggtgt cgtaaacgcg acgattacaa
    29941 accgctgtgg cgtactccgg ctttcgggct gatgaccacc ctcgccattg cgtcaagcct
    30001 cattctggtc gcctcaacct ttgtctgggc accgattccc ggccttatct gcgccgtcat
    30061 cgttattgct actggtctgc ctgcttacgc cttctgggcg aagcgtagcc gccagctcaa
    30121 cgctttgtcg taatgttatc tggagaaaat aaaatgttgg atattgataa aagcaccgtg
    30181 gactttctgg tcaccgaaaa tatggttcag gaagtggaaa aagttctcag ccatgacgtt
    30241 ccgctggtgc acgccatcgt ggaagagatg gtgaagcgcg acattgatcg tatttatttc
    30301 gttgcctgcg gctcgccact caacgcggcg caaacggcga aacatctggc ggatcgcttt
    30361 tccgatcttc aggtctacgc catttccggc tgggagttct gcgataacac cccgtatcgc
    30421 ctcgacgatc gttgcgcggt gattggcgtt tctgactacg gtaaaacgga agaggtaatc
    30481 aaagcgctgg agctgggccg ggcctgcggc gcactcactg cggcgttcac caaacgcgcg
    30541 gacagcccga ttacctcggc ggcggaattt agcattgatt atcaggccga ctgtatctgg
    30601 gaaattcacc tgctgctctg ctacagcgtg gtgctggaga tgatcacccg cctcgcgccg
    30661 aacgcggaaa tcggcaaggt caaaaacgat ctcaagccgt tgccgaatgc gctcggtcat
    30721 ctggtacgca cctgggaaga aaaaggccgc cagcttggtg aactggccag ccagtggccg
    30781 atgatttata ccgttgctgc gggtccgctg cgtccgctgg gttacaaaga aggcattgta
    30841 acgctgatgg aatttacctg gacgcacggc tgcgtgattg agagcggaga gttccgccat
    30901 ggcccgctgg agattgtcga accgggcgtt ccgttcctgt tcctgctcgg caatgatgaa
    30961 agtcgccaca ccaccgaacg cgccattaac tttgttaaac agcgtactga caacgtgatc
    31021 gtcatcgatt acgccgaaat ttcgcaaggg ctgcacccgt ggctggcacc gttcctgatg
    31081 ttcgtgccaa tggagtggct ctgctactac ctgtctattt acaaagatca caacccggat
    31141 gaacgccgct attacggtgg tctggtggaa tattaatccc tctcccggcc cggcaacggg
    31201 ccggatttac gcaaggagtt acccgatgaa aacaggtatg tttacctgcg gccaccagcg
    31261 gctgcctatt gaacacgcat ttcgtgatgc aagcgagctg ggttacgacg gcatcgaaat
    31321 ttggggcggt cgcccgcacg cgttcgcgcc ggacttaaaa gcgggcggca tcaaacaaat
    31381 caaggcgctg gcgcagacgt atcagatgcc gattatcggc tatacgccag aaaccaacgg
    31441 ctatccgtat aacatgatgc tgggcgatga acatatgcgt cgcgaaagcc tcgacatgat
    31501 caagctggcg atggatatgg caaaagagat gaacgcgggt tatacgctga tttccgcggc
    31561 ccacgcaggc tatctcacgc cacctaatgt tatctgggga cggctggcag agaacctaag
    31621 cgaactgtgt gagtacgcgg aaaacatcgg catggatcta atcctcgaac cgttaacgcc
    31681 gtatgaatcg aacgtcgtgt gtaatgctaa tgatgtgctt catgcgctgg cgctggtgcc
    31741 ttcgccgcgc ctgttcagca tggtcgacat ttgcgcgccg tatgtccagg cggaaccggt
    31801 gatgagttat ttcgacaaac tgggcgataa attatcgtca tctgcatatt gtcgacagcg
    31861 acggggccag cgacacgcat tacattcctg gagaaggcaa aatgccgctg cgggaactga
    31921 tgcgcgatat tattgagcgg ggctatgagg gttactgtac ggtggagctg gtgacgatgt
    31981 atatgaacga gcccagactc tatgcccgcc aggcgctgga acgctttcgc gcgctgctgc
    32041 cggaggatga gagatgaaaa ccctggcgac aatcggcgat aactgcgtcg atatctaccc
    32101 gcaactgaat aaagcgtttt ctggcggtaa tgcggtcaat gtggcggtgt actgcactcg
    32161 ctacggcata cagccgggat gcattacctt ggtgggtgac gatgactacg gcacaaagct
    32221 gaagcaggat ctcgcccgca tgggcgtcga tatcagccat gtccatacga aacacggcgt
    32281 taccgcacaa actcaggtgg aactgcacga caatgatcgc gtttttggcg actacaccga
    32341 aggcgtgatg gccgactttg ccctgagtga agaggattac gcctggctgg cgcagtatga
    32401 cattgtgcac gcggcaatct ggggacatgc ggaagacgca ttcccacagc tgcacgctgc
    32461 gggcaaactt accgctttcg acttctccga caagtgggac agcccgctct ggcagacact
    32521 ggtgccgcat ctcgattttg cctttgcctc cgcaccgcaa gaagacgaag cgctgcgtct
    32581 gaagatgaaa gcgattgttg cccgtggcgc aggaacagtg attgtcacgc tgggtgaaaa
    32641 cggcagcatt gcctgggatg gcgcgcagtt ctggcgtcag gctcctgaac cggtgacggt
    32701 tatcgacacc atgggtgccg gagattcgtt cattgccgga ttcctttgcg gctggtctgc
    32761 ggggatgaca ttaccgcagg cgatggcgca gggaacggcg tgcgcggcga aaaccattca
    32821 gtatcacggt gcctggtagg tataacgttg gcgtgagcat ctccgcgcca acgtgctgtt
    32881 acttgccgga aaacgaccct ataatccgtg taattcattc tttatttcag ggtcgattat
    32941 gtcagctacg gaccgctact ctcatcaact cctctacgct accgtccgcc agcggctgct
    33001 ggatgatatc gcgcaggggg tttaccaggc cgggcaacag atccctaccg aaaacgagct
    33061 ttgtacgcaa tataacgtca gccgcattac tattcgcaaa gctatcagcg acttagtggc
    33121 agacggcgta ctgatccgct ggcagggcaa aggcaccttt gtacaaagcc agaaagttga
    33181 aaacgccctg cttactgtca gtggttttac cgattttggc gtctcacaag gcaaggcgac
    33241 gaaagagaaa gtgatcgaac aggaacgggt cagcgccgcg ccgttttgcg aaaagctgaa
    33301 catccccgga aacagcgaag tgttccatct ctgccgggtg atgtatctcg ataaagagcc
    33361 gctgtttatt gatagttcat ggatcccgct gtcgcgttat cctgactttg atgagattta
    33421 cgtcgaagga agctccacct atcagttatt tcaggagcgt tttgacacgc gagtggtcag
    33481 cgacaaaaag accatcgata tctttgccgc cacccgcccg caggcaaaat ggctgaaatg
    33541 cgaactgggc gaaccgttgt ttcgcatcag caaaatcgcc tttgatcaga atgacaaacc
    33601 ggtgcacgtc tccgaactct tctgccgcgc caatcgcatc accttaacga ttgataataa
    33661 aagacattaa ccgtaggccg gataagatgc gccagcatcg catccggcga tgctggcgcg
    33721 ttgaatttta catcccgtac gttcccctca ccctaaccct ctccccaaag gggcgagggg
    33781 accgttcagt acggaattta gcttgacggt gctatccagc attaaatact ggcaatactc
    33841 tcacgcaaaa tccgcagcac cgtctcttca ccgttgcgat tcgggttaat gcgaatcgca
    33901 caatgttctg attgtggatt cgcctggcga aacgttccgg aaaggcgata aaagagcggc
    33961 gggatttcat atttcgactc tgcacccacc gggtaaggca aggcaccgcg cttttgcgcc
    34021 tcttccagca ctctggcggc aatcggctga tgaaactcga caatcaacac cttcgactgc
    34081 gcattagcaa tcaccgcgct tttcacttcc ggcaccgcac caccgttaag caaagccagc
    34141 aaccgttcag atacccccgc ctgcaccgcg tgcatcactg gcgcaaacac cagcccacac
    34201 aagacttcca gcgcctgagc gccctgaatc tggctgccgc cggaatagag cgtggagcga
    34261 atccgactaa taacatcggc atcgcctacc accgcgccaa caccctccgg cccaaacagc
    34321 ttgaagcagg aaaaagtcga gacatttgcc ccgcattcgc agccaattcg cgccactttc
    34381 atcaccgcat agttgtcatc ggttaatgca ggaacgccag ccgagcgcaa tgttgccagc
    34441 acatctgcca gaatgtagcc gtcctgcggc tgctggcgcg tatgctgcac cagcgccgca
    34501 tccggtagtt gctcgtcgac gacctgcttc agtgccgaca ggtcattgaa atcagcagta
    34561 ataagcgtca gccccatctg ctcaataatg acctgtgtcg tcgggtaaac aggcgcatca
    34621 tgtaccagaa gacgctgccc cggtttgagc aaagccgcca acgccgcacg aatcgcgcca
    34681 gtacccgcac cctgcaccag cgccgcagcc tgtgcgtgaa atgcatcagc cagcacctgt
    34741 tccacacgct gggtaacgcg cggttgattc agtcctggcg ttaagcctaa atcaccgcca
    34801 gtaagaaact cgctgccggg gaaatggcga caaatgctat ccaccagcgc aaactgcttt
    34861 tgctgcgcct caataatcgt cagactttgc agaggaaacg tcttcatagg tgccttcctt
    34921 aagccggaac gaacaggcca agccagtaaa gaacattcag cagaataccg gtgatcatta
    34981 ctgccacgac cggtgccgcc attttctgta ccggacggcc cagggattcg ttgaggaagt
    35041 aaatggcaac cgcgatagag aatccggtat aacccgccat cttaattgct gcgaaaatcg
    35101 aaccgaccag cagcgccact tccatcagca tattcatggc gttacggatg ttatccgacg
    35161 cattacgcac cgacgggtag cgtcccagcc atttgccgat cgaacgaagc agcaagactt
    35221 ccgccgaaat aaccactgcg cctaataccg ctgcaactat cggattcggc gagagatagc
    35281 ccaccgcata aacaaaggta aagcccgcaa ctgcatacac acccgttgct aaggcggtgg
    35341 tggcaatcaa cggtacaaaa cccagcccgc gcataaattc tgccagtgcc gcctggttga
    35401 tcagcgtttg cgattgttcc ggcgttacgc ctgcggagta cgctttctcc agagtgaaga
    35461 tcgacacttc actgccggcg aaaatcttca tgctggcaac ggcggcaatc aatgctccca
    35521 cgatggcgat atagggtaag tttttgataa tccgcgacgt gcgttcttca aacaccgaaa
    35581 gcccgctggc atcaatatca ttttcatcac gatgacgcag gtcgtgagtt atcgcgatcc
    35641 ccagcagcat caccatgcca ataaagattt cgatggattc agggttaaga tgtgggaaat
    35701 agcgcacgac taccacacgg gtcatcagaa caaccacggc ggcgaccaga ctttgcttcc
    35761 agccaaactg gtagaaaatc gccaccagcg ggaacagtgc aaaagcagaa accaccggcg
    35821 agcttaattc cccgaggcta cctaatacat ccaccggcag cgcggtcagc agctggttta
    35881 ctggcaacag gcaagtaagg atcaacacgc cccagatagc gccaagacca aacgccatca
    35941 ggctgtttat cgccagtacg ccgaggatat cggtaggaag aaagagtaac catgcgttga
    36001 gcagcccggt tttcagggta aaagagatcc ccaccgaggc cacaaaacca atgctcagac
    36061 caaaagcgat actccccgcc tcgcgacggt tcatatagcc ttcaatcagt tgcggcagga
    36121 tcgggcggat accgtcatga aaaacagccg ccgagcgatg cgccagaagc gatgtcatac
    36181 ccgtcaggca cgccaccacg ataatctgaa tatacagatc catagtcgtg ccttttattt
    36241 cagatggtta atcagcatcg gaatcgcgtg ttcgacgtgc tcaacggaaa ggccaaacgc
    36301 cacttttcct tcagcgatca ttttggcgat atgctcgtct ttcgctttaa tgcctggttt
    36361 ggcaatggtg caacttttgt tatagccaat caccgcgatg gcaatcgaca atgccgcgcc
    36421 cgcaccggta ttacacgcac cgatgtagta atccagttgc ccggatttca ctttcattgc
    36481 cgcttccatg tcgttgtgaa tgaaaacttc aaaactgcca ggagccgtcg cttcaatggt
    36541 ttttttaatc tgctcacgct gtaagcctgc aacgccaatc tttttcatga gtctgtcctt
    36601 attggaaaaa ttgagaggga gtttcacgta acatcacatc cacatcggcc tgactgaatc
    36661 ctgactggcg caattgcgga ataaaggtgg tcaataaata gtcatagcca taaccaccat
    36721 tggcttttaa atgggagcgg cgcgtaatat ccatcgacag catgacgcgg ttcagcaacc
    36781 cacggtcacg tagcgcgtgg agcatcgcaa tgcgcttttc gtccgggtag taactgttct
    36841 taccaatggt gtcgaactgc acgtacgcgc cgagatcgat catcttcaaa atgttgtcga
    36901 ggttgtcttt cagatcgcag tgaccaacgg tgacgcgcga aagatcaact ccgtgggctt
    36961 gcaacagagc cagttgttcc agccccatgg tgctgaacga cgtatgcgtg gagatcgggc
    37021 gtccggtctg gttatgcgcc agcgcagcgg caataaatac cttctcttcc agcggcgtaa
    37081 tctttccttc gctggagccg atctccgcga tgatcccggc tttcagctcc gtgccatcga
    37141 taccctgttc aatttcatcg accatctcct gcgccagttc ctgcacgctg cgggtcacca
    37201 catgttccgg gaaaaacgca tcctggtaat aaccagtaca ggccaccacg ttgatcccgg
    37261 tttcgcgcat tacaccgagc ataaattgcg cattgcgccc catgtaacgg ttggtcatct
    37321 caatcacatt acgtacaccc cgggtcatca ggtcgttcat ctcctggcaa atgaacgcat
    37381 actgatcaag gcggcagtcc acgttgtttt taaagccgga gagatcaata tgcagatgct
    37441 catgggccag ggtgtaaccc gtcggatcaa aactcatagg gtgtctcctg caaaccggag
    37501 ggaggaaaga aaagagcgac cattttgtgg cggtggcgca cggaaaaatt cacacacggt
    37561 agctcccaca tcagaaagcg tggtgcgcac accgagctgc gtagcagcca ggccttgctg
    37621 ataaaccagc agcggcacca cttcgcgggt atggtggctg tgaccaatgg tcggatcgtt
    37681 gccgtgatcc gccatcacga ccaggcaatc atctggctgc atcgcatcaa caagccgggc
    37741 aaggttacgg tcaacgacct gcaaacgttc ggcataacgt gcgacgtctt ctgcatgacc
    37801 agcgaggtcg gtttcctgga tattggtgca aataaacgcc gtcggatggg tgttaaattc
    37861 gtcgagggtg atatccataa tccgctggct atccaccagg ttttgccagc tcacgccata
    37921 aggattgctg acgatatctg ccaccttacc caccagcacg gttggcacgc ccgcctcata
    37981 cagtttttgc ggcacctgca cttttttatc gacaccgtag cccatatgca cgacctggaa
    38041 gccgttgtca taagcgccag aacgcggcgc attgatacca ataaagcgcc cttctttgct
    38101 ttctgcggca tcgagaatgc gttgactgtc ggttaacagg ccaccaaatg taatgacccg
    38161 accgacctgt acctgctcac gcacgatacg accaattttg attgcgtcgt caaaagagat
    38221 cacagagaga ttggcggtaa tgttatagac ttggcctaaa tccgcctcga gattatcgcc
    38281 aatcgcaacc gcctgattga cccacagaaa ttacagttca tcgccacggc gctccacctg
    38341 ccaaccggcg gatactaatg cctgctcaac acggtcaatc acatcgcgaa aaggcatccg
    38401 cagcggcggt aacgggcgcg tgcctaaaat ttcctgatgc cccataaagg tatcgccacc
    38461 ttcatgttgc agctctgcca cgccccaggt tgcggaatct gacggctgca tatcgcctgg
    38521 cgcataaccc aatgcgttga ttagccccag cttctccagc gttggtagct gcaaatgcgg
    38581 caactggctc aggatgtgac cacatgtatt cgctcccgca tcttgcggac gcaccagcgt
    38641 gacatctttc attgccccta cgccaaagct atcaatcact aacaccacaa atcgcgccat
    38701 tacgccccca gagagtttcc aagactgtca taacgaccaa ctattttcgg ttcgccacgg
    38761 tgaataccgg acaccagcac cacatcgcta cgggtgacaa aaatctgcgt gcggaaacag
    38821 agcaccactg cactgcttac cggaaactcg cctgccagcg acagggtgta gtcgatactg
    38881 ctgtcatcca cagttttgag attggtttca gtaatctttt gattttctgg cgtaaacacc
    38941 aacgcatgtt gcgcatgacc acgacggtag taaccgccgc cgtagcagta gctgtcgcca
    39001 cggaaatgat gggagatttc actcaaccag agcatcgcga tacgttcaga ctgatcgccc
    39061 tgctggtttg ccggaatagt gcccgtcagc gcatgaccgg gttcggcatg agtcacaccg
    39121 tattacgcca gtaatggcag cgaagtgcag ctggtcgctg aaggcgcgtt cagttgctca
    39181 agtgcaatac cagatttcgc cagttgatcc cgtgcctgta tcagcgtgtg aagattcggt
    39241 gtcggcaaaa cttttccggc agcctcatcc caaagcaggc aagggaaatg ggtaagtccg
    39301 gctaaatgca gccccggcag tttcttaatt tcagcaacca cctcgtgcag cgaatgctgt
    39361 acaaaaccgc tctcctgccc cggataaaga aaatcatcgt cgctataaac tttgagcagc
    39421 acaaactgca cacgtccggc cttcaccgct gccgcagaaa cttcccgcgc tttgtcgaga
    39481 gtaaacacgg tgatgacgtc ggtgccctgt tcaacggcgt cagcaacctg atgacaaggg
    39541 atttgtacca gatgcccctg atgtgccacc gacaaaccag cgcggcgcat gactcgcgcc
    39601 tctttgtaat ccaccgccac aatgccgctg taacctaatg ccagcaattt ttccgccagc
    39661 catgggttac ggccaaactg cttggtcatc agatacagtt caatgccgta aagccgcgcc
    39721 gtctcaatca gtcgcttacc gttttccagt acctgatcca cgtcgatcac ccagctgtcc
    39781 ggggcgatct tgccctgctg ccacaaactt agtgcggcgg aaatcagcgc cgggttctgg
    39841 cgtttcaatg cttctacaaa catcttgttg cctcgtttct gttccttgaa ttaaatattc
    39901 atttttttga atatttaggt tgtgcgacgt ggatttgtcg ggagcttcaa acctcttcgt
    39961 tagcagccat ccataatcca taaaggttgg ccagcaaata gccttcttca cacgggttaa
    40021 cttccagtgc gaattccttc aacaacacct gatgcaattg cacaacggct tgccagtggc
    40081 tggattgcgc cagctctgcc agcaattcgt tatccagcgg ctctatttct tcaccacggc
    40141 gactgcgcat cagtgcactc gccatatgtg tcatcgccat cgttccttgc tcactgcgca
    40201 ccggcagatg gcactctgtt tccagtacgt tgacgacctg catcatgcct ttgcagatgt
    40261 ccttatcaat aacgcctgcc tcgcaaagca ggttgagtct ggtttccata tcagatgcct
    40321 ttcgtttaat aactcggttc ctgttcccca ctcactaccc gttgttgatg ggcgagcagg
    40381 gcgtgtttat ctacaacggc acgcagcagt tgttgcattg ggtaatcatc gactcgcgtc
    40441 aggaccacgg cttcggtggc ctgtaaaaat cgcggatcgt ctgtgagcgg cgtcgcctct
    40501 aatcccagca tggtcagttc gttttccgcc accacgttcc agataaccgc atcgacatcg
    40561 cctttaacaa tgcgttgtaa actctcgtga taagagagat cgacacgttc cacatcacta
    40621 tcgccaaaaa agacatcggt catgattttc tgatccgccg aacggttatc cagccccacg
    40681 cgcttcacgt ttgcggactc gcctttacgg caaatcaact ggtgctcgcc aacgtaggtg
    40741 tgcggcccca actccagcgc gaggcataac cctttttgcg tgagataact ttccgccgcc
    40801 agtcgggaaa ccaccgccat gtcatacacg ccgttaagta aacactccac gcgaatatcc
    40861 gcgccgcgca tatgcgcata gtaaaaagga atgccatcaa actgagcttt cagcccgctc
    40921 gctaaacctt cgtacaagcg ggtatagggc aaaggcatcg cacataccac gttgttgata
    40981 tccacatgac tcagcaatgc tttgttatcc atctcgacca gataactgcc attgcgccca
    41041 cggcgttcaa tccgtatcgc tccgcttgat tccaacgttt tcaacgcggc ctgcgtcagg
    41101 ccaaccgatg aacggcattc agttgccagc tcatctatgg ttttcagtcg attaccgcat
    41161 ttttcaccca acaaataacg ggccagcgtc gtgatgacga cgccttcttt tttgataaac
    41221 gttcgacgca taatatattt tcaataaaat gaatatttat atcttcatta aaatgaagat
    41281 aagtggcaac gacgagatgt ggaaacggcg aggcgcttca cattttttcg gattattgca
    41341 aatgaggata aaaaaaccgg gtttccccgg tttttggcat taatgttaaa ttacggcttc
    41401 gccacaaaac caatcgcttc gtacaccgct ttcagcgtac gggaagcgtg tacactggct
    41461 ttttctgcgc cgtctttcat cacctgttgc aggaaggctt catcgttgcg gaaacggtga
    41521 tagcgttcct gcaattcagt cagcataccg gaaacggcat cagccacttc acctttcaga
    41581 tgaccataca tcttgccttc gaactgtttt tccagttctg ggatgctctg gcccgttacc
    41641 gcagaaagga tatccagcag gttggaaacg cccgctttgt tctgcacatc gtagcgaact
    41701 accggcggct cgtcggagtc agtgaccgcg cgtttgattt tcttcactac cgatttcgga
    41761 tcttccagca ggccgataac gttattgcgg ttatcgtcag acttggacat cttcttggtc
    41821 ggctccagca gcgacattac gcgcgcgcca gatttcggaa taaacggctc cggcacctta
    41881 aagatctcgc catacagcgc gttgaaacgc tgggcgatat cgcgactcag ttccaggtgc
    41941 tgtttctggt cttcacccac cggtaccaga ttagtttgat acagcaggat gtccgcagcc
    42001 atcagcaccg gatagtcaaa cagaccagcg ttgatgttct cggcataacg cgcagattta
    42061 tctttaaact gcgtcatgcg actcagttcg ccgaagtagg tataacagtt cagtgcccag
    42121 cctaactgcg catgttctgg tacgtgggac tgaacaaaaa tggtgctttt ctcaggatcg
    42181 ataccacaag ccagatacaa ggccagcgta tccagcgtcg ctttacgcag cttctgtgca
    42241 tcctggcgca cggtgatcgc gtgttggtca acgatacagt aaatgcaatg gtagtcatcc
    42301 tgcatgttta cccactgacg cagcgcaccc atgtagttac caatggtcaa ttcacctgag
    42361 ggctgtgcgc cactaaaaac gatgggctta gtcatttttc gattcctgat tttcgctatg
    42421 cggaagccct aatgcgggca gaaggtcatt tatagactga taaattacat caggctggct
    42481 gagatcgata gcctcgccgt agttatatcc gtaggttaag ccaactgatg ggcaacctgc
    42541 cgcttttgcc gcctgaatat cattgcgtga gtcgccgaca aacagcatct gttgtggggc
    42601 aattcccatc cgctcagcca ccagtaacag cgggtccgga tgcggttttt tgttttgcac
    42661 atcatcgccg ccgataacca cgctaaagta tttggcaata tctaaggctt cgagcagcgg
    42721 tgcgacgaac ggcgtcggtt tgttggtgac caggcctagc ggcaggcctt tagcctgcaa
    42781 cgcgcccaac gtatcggcaa cgtgcgggaa caaaaacgtc ccctcttcgg caacctcgcc
    42841 atagtagcga tcgaacagtt tacgcagaat acgtacctgt tcttctgccg gaatgtcgtc
    42901 atcaacgggc ggtttaccca ttgttttacg ctgagtcgca cgttcctgac gcgcccaggt
    42961 caatgcgcgc tccatcagaa catctgcgcc gttaccaatc caggtaataa cgcgttcttc
    43021 acctgcgatg ggcaactcca gcgcatacag cgccatatct accgcagcag caagaccggg
    43081 agcactgtcg accagcgtac catcaagatc aaaagcgacg ccgcgaatat cttcaaactt
    43141 attcatgact tacctttgcc agttcactgc gcatttcatc aatgactttt ttgtagtctg
    43201 gctggtcgaa gattgccgaa ccggcgacga acatatccgc gcccgccgca gcgatttcgc
    43261 caatgttgtt caccttcacg ccaccgtcca cttccagacg aatgtcaaag ccagactcgt
    43321 cgatacggcg acgtacttcg cgcagtttat ccagtgtttg aggaatgaaa gactgaccgc
    43381 cgaaaccagg attgacggac atcagcagga tcacatccag cttatccatc acgtaatcca
    43441 gatagctcag aggtgtcgcc gggttaaata ccagacccgc tttacagcca ttttctttaa
    43501 tcagttgcag cgtgcggtca acatgctcgg aggcttctgg atgaaaggta atgatgctgg
    43561 caccagcggc agcgaaatca ggcacaatgc gatcgacggg tttcaccatc agatgtacgt
    43621 cgataggggc ggtaatgcca tagttacgca aggatttcag caccattggc ccaatcgtca
    43681 gattgggaac atagtggtta tccatgacgt caaaatgcac gacatcagcg ccagctgcca
    43741 gggcttttgc ggtatcttca cccaggcggg caaaatcagc cgacagaatt gagggggcaa
    43801 tcaaatactg tttcatccgc ttctccttga gaattatttt ttcgcgggtg aaacgactcc
    43861 tggtttgtac aaagccagca gttcgtccac ctttttacgt gtgccgccgt tgctgcttat
    43921 actgcgtcga actttgacga catgcaattt tgcgcgctga taccactcac gcgttaacat
    43981 cgtatcgtga ttggagatca gcactggaat atggcgctca accagacctt cggcgatctc
    44041 cgccagatgc gcttgttgtt caagcgtaaa actgtttgtg tgatacgccg taaagttggc
    44101 ggtcgcagac agcggtgcat aaggcggatc gcaatagacg acggatgcat catctgcgcg
    44161 cgccatgcta tcggcgtaag actcacaata gaaaaaggca ttctgcgctt tttcagcgaa
    44221 gtgatacaac tctgcttccg ggaaataggt ttttttgtag cggccaaacg gcacgttaaa
    44281 ctcaccgcgc agattgtaac gacacaggcc gttgtaaccg tagcggttca aatataaaaa
    44341 cagtaccgcc cgacggaacg gatcctggct tttgttgaac tcttcgcgga actgatagta
    44401 aacctcggcg caatttgttt cgggaacaaa cagctcgcgt gcggcctgta cgtactcatc
    44461 agtacgcatc ttcacaatgt tatagagact gatcaggtcg ctattgatat cggcaaggat
    44521 ataacgagaa aagtcggtgt tgagaaacac cgacccggca cctacaaaag gctcaaccag
    44581 acattcgccc ttgggcaaat gccgtttaat atcatcaagc aggggatact tgccccctgc
    44641 ccacttcaaa aaagcgcgat ttttcttcat gctgactaac taattacacc ttctccggct
    44701 gtggagaaag ctccgacagc atccagcgct tcaggcagct cccgcaacga taaaccattg
    44761 cgggagataa ccttgattac ttcagatcgg cctgtacctg acgcagcggt ttcgcccacg
    44821 ggtttttggc ctgaacatct gctggcaatg tagataccgc ttttttcgcc tcttctttcg
    44881 aagcgtacac gccagaaacc aggacatacc acggctgacc attacgcgcc gtttcataga
    44941 caacgtagtt tttcagattc tctttcttcg cccaaccgtt caggttgtcg tagttagagg
    45001 aactgctcag ctgcagagtg taatggctgg acggtgccga tttcaacgaa ccaacattac
    45061 ctgcggtctt ccctccagcg gctggtgttg ccgtggtttg cgccggggat gccgtctgta
    45121 ctggagccgt agtcgccgtc tcttttggtg ctggcgtaga agtcgcagtc ggtgccttcg
    45181 tgctcgccac aggggcagct ggttcagtac gcttcggcgt ctgtgctacc ggcttcggct
    45241 ccgttttcac ggttgcttgc ggttttttcg gttcaatcac cgcctgctga cgagctgggc
    45301 gcgtagtgga cggacgttca gcggtttgcg ttttcgccgt gtcacgcgat gcattgccat
    45361 tgcgaacagg cgcgacagtc gcgggttcgg tcggcaatgt ggaattgacc gccacattgt
    45421 tcaactgttg ctgattttgt ggctgggtca gcgcattgtt caggtcaccc tgcacttcaa
    45481 cacgttgttg accatccgtt gccgccgggg tttgcccttg agtcggcgta gaagagatcg
    45541 gtggcagaga aacatcctgc tgagtatttt ccgcagacgt ggttcccggc gctggctgca
    45601 ccccattcgc ctgatcggtc gcattgcctg caagatcaat actcttctcg ccagacgcgg
    45661 tttgatcgct ggaagaggtc gagggggctt ttagcgcaga accgataccg atgatcaaca
    45721 gcagtagaac cagaatgccg acgcccatca tcatatactg acgagaagcg ggtttactgg
    45781 ctgctttttt gcgcttacgc ggacgacgct ctacgcgctc ttcatccacg gtttcatctt
    45841 cggattcgtc aatttcttct tcgatttccg gttcctcatt gcgctctttt tgcgcacgag
    45901 tcggacggcg atcgtcagtg tcatcaagtt caatatcatc aaaattgatc tgcggttcgc
    45961 cacgttcagt acgctcagaa cgttcagaag attgacgaga acgaccagta cgacgatcgc
    46021 tgggatcggg tttcagctcg tcttctggtt tgaattcatc catttaacac cccactaaaa
    46081 ggttaatgct taccacgttg caattaacct gaagctaata gaccgcttga taagcggcct
    46141 gacctttctt gttgttacgc tgattgacaa tcggcaatgg cgttaagaac aagctcgtgc
    46201 gaaacgccgc tgcgaacttc actcttacca attgccaacg gaagaattaa gcgtatctct
    46261 cccgcaagga ctttcttgtc acgcagcata tgcggtaaat acgcctgcgc ggacatttcg
    46321 cgcggcccat tgaccggtaa cccagcccgc ttgagcaggg ttataatacg ctgcgtttcg
    46381 gcagaactaa actgcccgag acgttccgac gtccgcgccg ccatcaccat acccgcagcg
    46441 accgcttcac catgtaacca attgccatac cccatttcag cttcaatggc atgaccaaag
    46501 gtgtgtccca gattcagtaa agcacgtaac ccggtttcgc gctcgtcggc ggcgacaact
    46561 tctgccttca gttcacaaca acggcgaata cagtacgcca ttgccggacc gtccagacgc
    46621 aacaacgcat ccagattctc ttccagccag ttaaaaaacg caccgtcaag aataatgccg
    46681 tatttgatga cttctgccag ccccgacgct aactcacgcg ggggaagcgt tttcagacag
    46741 tcgagatcca ccaccactga agcaggctgg tagaacgcgc caatcatgtt tttaccgagg
    46801 ggatggttga ccgcagtttt gccgccaacg gaggaatcga cctgcgacag taacgtcgtc
    46861 gggacttgaa tgaaacgaac accgcgctga taactcgccg ccgcgaagcc ggtcagatcg
    46921 cccactacgc cgccgccaag cgccaccagc gtagtatcgc gaccatgcgg cttttgtaac
    46981 aacgccgtaa agacggtatc gagtacagcc aggcttttat actgctcgcc gtcagggagg
    47041 ataacgctat cgacattaac acccgcctgt tcaagtacgc cgcggacctt atcgagatac
    47101 agaggagcca gggtttcgtt ggtgaccaac atgacctgct cgcccgattt cagcggtaag
    47161 aatgaagctg gttcattaaa caaaccagat gcgatggtaa ttgggtaact acgttccccg
    47221 agagtaacaa caatcctctc cataacgcga catccacctt aattactgta cccgcagacg
    47281 agtgtatata aagccagaat tagttgcttt ccagcatgtg aataatctgg tttgcaacca
    47341 ctttagcgct ttgatcatca gtacgaatgg tcacgtcggc aatctcttca tacagcggat
    47401 tgcgttcatt ggccaacgct tccagaactt cacgtggcgg tgtttcaacg tgcagcaacg
    47461 ggcgtttttt atcacgctgc gtgcgtgcaa gttgcttttc gatggtcgtt tcaagataaa
    47521 cgacaacgcc acgagcggaa agacggttac gcgtttcacg ggatttcaca gagccaccgc
    47581 cagtagccag cacaataccc tgtttctcgg tcaactcatt gatgaccttt tcttcgcgat
    47641 cgcggaagcc ttcttcgcct tctaaatcga aaacccagcc cacatcagct ccggttcgtt
    47701 tctcaatctc ttgatcggaa tcgtaaaatt ccatattgag ttgttgagct aactggcgcc
    47761 caatagtgct ttttccggca cccataggcc caaccagaaa gatattgcgt ttctctgcca
    47821 ttttttcggt actactaaga ctattcgtta atgataaacc cgcttcgctc agagagcgcc
    47881 gcaggacatg aactgaaacc tcataagata ttgcgagagt cagactgaaa attatctcaa
    47941 tactccagcg ggtttggcaa ctgaataaat cacccatccc tctgcatatc tggtcgctgc
    48001 aagcgcgctg ccttgctacc accgctctgg cgataaatca ccgggtaaga ttagcgtaaa
    48061 aaagacagca aaatgccgcc tgaatgataa atcatcatca tggaacgcag gcagcgaaga
    48121 gcaccaactc tcaaatcggt actccttgta tgctaaatac ctgcgcgcgt caaatagatg
    48181 aaacacgttc agcgtaaaaa cattaccgct tttacggctg tttactcact ggaaaccagt
    48241 cgtggcgtga taaacaccac taactcgcgt cgttcatctt cttttccgtc atgacgaaat
    48301 aattgcccga accagggaat gtcgccaagc aacggtacgc tatcctgacc cgatttattt
    48361 ttacgggtaa aaatgccgcc cagcgccaac gtttctccgc ttttgacctc gacctgcgtt
    48421 tcgatctcct gcttatcaat cgccagcact tcgccatcgg cctgctgtag cacctgcccc
    48481 ggaacgttct ggctgatgtg taatttcagc cggatgcgac ctttttgtaa cactgtgggc
    48541 gtaacttcca tccccaggac ggcctcttta aattccaccg acgtcgcgcc actttccccg
    48601 ctggaaacct gatatggaat ttcgctcccc tgtttaatgc tggcaggctg aagatgtgag
    48661 gccagcagac gcggactggc gataatatcc agctgctgtt tttgttcgag cgcggaaagc
    48721 tcaagatcca gcaagcgtcc gttgatgcgc ccaatgttaa aaccgacatg cgttgtcgcc
    48781 gtcgctacgg agaggtcgct accaagcgtg gtgacttgcc caacgccacc agcgtgttgc
    48841 gcatcggcca gcgtccattt cacgcctaac tcacgcaaac ttttttcatt aatggtgaca
    48901 atatgcgccg acagctcaac ctgcccgacc ggcagatcca tttgcgctac ccactgttca
    48961 agcgcgctta acgccgtttt gttatctcgt agcaaaaggc gattggtgcg tttatcgacg
    49021 gtcatactcc ctttggcact cagtagcttc tcccccgctt tcgccagttc tcccgcgtcg
    49081 gcgtattgca gggttatact gcgattttcc agcggcagat ttgcctgcgc ccgcgcctgc
    49141 tccgcctcct ggcgggcgat attgttattc tgccaggcaa tggaatgcac tgagagaatg
    49201 ttgccttcct gccgcgttat cagtccggcg cttttcacta cagtttgtag tgcctgcttc
    49261 cagggaacat ctgttagatg taacgacacc gtaccgctga cgtctggcga cacgaccagg
    49321 ttcaacttct cctgttcagc cagcgcctgc aacacctgag ctaccggaac gtcatccacc
    49381 atcagcgtca ctttttgcgg ctttgccgcc tgtacgccgg gtatcagcat caacagtagt
    49441 gcggctatcc attgcttcat ttgtatctcc ttgccgttgc cacaaccatt gtggcggttc
    49501 gcagtttgtc ccggtaccca gcgttagtac gtctggcgtc agctgtaaaa ttgtccagcc
    49561 gttttccagc acatcgtttt gctgcacccg tcgccatttc ttttgcccgt cttttattac
    49621 accgatgatg cgctcgcctc gccctaccat cccctgatag cgccactggc taagttcgct
    49681 aatccggcat agatcttccg gcggtttaaa agggtcacgc ataccggtta aaaggcacaa
    49741 tacaatacct gccaacaacc agcgtttaac cctcatgcgg cgtctccagt tgtagcgtga
    49801 acaaaagatc atcaccttcc acgcttaatg aaaaacggct cacgctgacg ttgcgctctg
    49861 ccagccgtgt aaatgccgac ggcactgctt cccacagcgt tttcaacgcc aactcgcctc
    49921 cctgcgcgga tggatgccag gaaaccagtt gcgcgccggg caactgaaaa tccagtggcg
    49981 aaaagggcag cgttttttct tcgctgaagg gagtggtgtc aaccaggcga tacagtgtgg
    50041 cccactgctg gtgatgactt gcccgtagtc gaattaatgc gtcgcgctct tcatggtgtg
    50101 tcgatgacag aaaaatgagc gtaactaaca tcagcaacca gaatgcccag caaaactggc
    50161 ggaggcgggg tgatgtggcg aaccaccagt caaagaacat gttcatcgct aaccttcctt
    50221 gttaactgat actcaaattg ccagcgtccc tgcgcatcct gctgcgtggc tccccgctga
    50281 ttgagatgaa aagacgcatc ctggcggagt gacgtttcta gtgcgtttaa cgcggtaatg
    50341 cttgttgtca gccccttgat ctccagcgtt ccctgctgcc agcttatcgt tgtcagccag
    50401 gcgtgctcgg gtaaaagcgc cgccagtgct tccagcgcag attgccagtc gcgggtaaat
    50461 tgtcgctggc gctgccgttg caaacgctgc tcacgaattt gttggcgctc cagcaaacgt
    50521 ggtttcgtta tctgtaagct gcgggcgagt tgttgttccg cctgaagcaa aacggtgttt
    50581 acgcgtgctt cggcgttgtc tgtcagacgc agtattagcg ttatcccgac ggccagcagc
    50641 agaggcgcaa cgaacatcag caaccagaaa cgaagaaaag cggtccggcg ttgctgtcgc
    50701 cagggcaaaa aattaattgg cgggttcatc agtacgcctt cccaagcgcc agccccagcg
    50761 cgatggcaaa gtctccacct ggcggcggta gcggcggctg acgaacagaa acggcctccc
    50821 agggatcaaa tccgccttcg ccacatatcg cgacgctttc gggatcaacg gataacgctg
    50881 ccgccagctc cttcgcgcta gtcatcccca ccgccagttt gcgcccccag cgacagcgtg
    50941 tcgcccacag ccactgttcg ttatcacgcc aggccagaca ttgctgatga gaaggtaaaa
    51001 aaggcaggaa tcgctgtaat gcactggcat ccggggtgat cgcgctcaca tgaacacgca
    51061 acctttctgc cagagtgagt agcgttgcca gctctttgct ttgcgcggca gtcacgttat
    51121 aggcgggact gagtgagtct tcgctgtaat cgaaacgcag ggagtccgga tccatatcca
    51181 gctcgcgggc catcgtccct gacagccagg ccgtttgctc ccgctcaccg agggacatcg
    51241 acgggcgcgg aaatgaccgc tgtaatgtgc gactggcggg aaacgccaac ataatgtgat
    51301 gacgctgcgg cagttcgcga ctccacggta acaacgtttt agccagccgc tgcgcatcaa
    51361 caatccgccc atctttgata atgtcgttct ccagcggcaa ccgccaccag cgttgcaaaa
    51421 agcattcttt tgcgccccgc acgatcgcaa ccgctactgc ttcttgctgt tgtaaatgca
    51481 aaccaatttg ccagatctta aatgccattg tgatgatctc cttatcaccc gtcactctga
    51541 cgggtatatc aatgcgtctg gcttgccttt atactaccgc acgtttgttt ataaactgcc
    51601 caaatgaaac taaatgggaa atttccagtg aagttcgtaa agtatttttt gatccttgca
    51661 gtctgttgca ttctgctggg agcaggctcg atttatggcc tataccgcta catcgagcca
    51721 caactgccgg atgtggcgac attaaaagat gttcgcctgc aaattccgat gcagatttac
    51781 agcgccgatg gcgagctgat tgctcaatac ggtgagaaac gtcgtattcc ggttacgttg
    51841 gatcaaatcc cgccggagat ggtgaaagcc tttatcgcga cggaagacag ccgcttctac
    51901 gagcatcacg gcgttgaccc ggtggggatc ttccgtgcgg caagtgtggc gctgttctcc
    51961 ggccacgcgt cacaaggggc gagtaccatt acccagcagc tggcgagaaa cttcttcctc
    52021 agtccagaac gcacgctgat gcgtaagatt aaggaagtct tcctcgcgat tcgcattgaa
    52081 cagttgctga cgaaagacga gatcctcgag ctttatctga acaagattta cctcggttac
    52141 cgcgcctatg gtgtcggtgc tgcggcacaa gtctatttcg gtaaaacggt cgaccaactg
    52201 acgctgaacg aaatggctgt gatagccggg ctgccgaaag cgccttccac ctttaatccg
    52261 ctctactcaa tggatcgtgc cgtcgcgcgg cgtaacgtcg tactgtcgcg gatgctggat
    52321 gaagggtata tcacccaaca acagttcgat cagacacgca ctgaggcgat taacgctaac
    52381 tatcacgcgc cggagattgc tttctctgcg ccgtacctga gcgaaatggt gcgccaggag
    52441 atgtataacc gttatggcga aagtgcctat gaagacggtt atcgcattta caccaccatc
    52501 acccgcaaag tgcagcaggc cgcacagcag gcggtacgta ataacgtgct ggactacgac
    52561 atgcgccacg gctatcgcgg cccggcaaat gtgctgtgga aagtgggcga gtcggcgtgg
    52621 gataacaaca agattaccga tacgctgaag gcgctgccga cctatggtcc gctgctgcct
    52681 gccgcagtca ccagcgccaa tcctcaggaa gcgacggcaa tgctggcgga cgggtcgact
    52741 gtcgcattga gtatggacgg cgttcgctgg gcgcgtcctt accgttcgga tactcagcag
    52801 ggaccgacgc cgcgtaaagt gaccgatgtt ctgcaaacgg gtcagcaaat ctgggttcgt
    52861 caggttggcg atgcatggtg gctggcacaa gtgccggaag tgaactcggc gctggtgtcg
    52921 atcaatccgc aaaatggtgc cgttatggcg ctggtcggtg gctttgattt caatcagagc
    52981 aagtttaacc gcgccaccca ggcactgcgt caggtgggtt ccaacatcaa accgttcctc
    53041 tacaccgcgg cgatggataa aggtctgacg ctggcaagta tgttgaacga tgtgccaatt
    53101 tctcgctggg atgcaggtgt cggttctgac tggcagccga agaactcacc accgcagtat
    53161 gctggtccaa ttcgcttacg tcaggggctg ggtcagtcga aaaacgtggt gatggtacgt
    53221 gcaatgcggg cgatgggcgt cgactacgct gcagaatatc tgcaacgctt cggcttcccg
    53281 gcgcaaaaca ttgtccacac cgaatcgctg gcgctgggtt cagcgtcctt caccccaatg
    53341 caggtggcgc gcggctacgc ggtcatggcg aacggcggct tcctggtgga cccgtggttt
    53401 atcagcaaaa ttgaaaacga tcagggcggc gtgattttcg aagcgaaacc gaaagtagcc
    53461 tgcccggaat gcgatattcc ggtgatttac ggtgatacgc agaaatcgaa cgtgctggaa
    53521 aataacgatg ttgaagatgt cgctatctcc cgcgagcagc agaatgtttc tgtaccaatg
    53581 ccgcagctgg agcaggcaaa tcaggcgtta gtggcgaaga ctggcgcgca ggagtacgca
    53641 ccgcacgtca tcaacactcc gctggcattc ctgattaaga gtgctttgaa caccaatatc
    53701 tttggtgaac caggctggca gggtaccggc tggcgtgcag gtcgtgattt gcagcgtcgc
    53761 gatatcggcg ggaaaaccgg taccactaac agttcgaaag atgcgtggtt ctcgggttac
    53821 ggtccgggcg ttgtgacctc ggtctggatt ggctttgatg atcaccgtcg taatctcggt
    53881 catacaacgg cttccggagc gattaaagat cagatctcag gttacgaagg cggtgccaag
    53941 agtgcccagc ctgcatggga cgcttatatg aaagccgttc ttgaaggtgt gccggagcag
    54001 ccgctgacgc cgcctccggg tattgtgacg gtgaatatcg atcgcagcac cgggcagtta
    54061 gctaatggtg gcaacagccg cgaagagtat ttcatcgaag gtacgcagcc gacacaacag
    54121 gcggtgcacg aggtgggaac aaccattatc gataatggtg aggcacagga attgttctga
    54181 ttaaaaaggc gcttcggcgc cttttcagtt ggctgacaaa gtgcatttgt ttatgccgga
    54241 tgcgggtgaa cgcgttatcc ggctaacaaa atcgtgaaaa atcaatatat tgcaggaacc
    54301 ccataagcct gataatcgtt gtgcatcagg caaacttcac gcatttacac tcgcccctgc
    54361 cctttcaacc attcgcgcac gaggaacagc gcgctgacat tacgcgcttc gttgaagtca
    54421 gggtcttcca gcaaatccat catatgcgcc agcggccagc gcacctgtgg tagcggctct
    54481 ggctcatcgc cttccagcga ttccgggtag agatcttgcg cgaccacgat attcattttg
    54541 ctggaaaagt aagacggtgc catgctgagc ttcttcaaaa aagtcagatc gttcgcacca
    54601 aatccaacct cttcttttag ctcgcggtta gcggcttcgt agacgctttc acccggatca
    54661 attaatcctt tcgaaaaacc taattcgtag gattcagttc ccactgcgta ttcgcggatc
    54721 aggatcaggt gatcgtccac aatcggcaca atcatcactg cttcccggtt ggttggacgc
    54781 attcgttcat aaacacgccg cacgccattg ctgaactcca gatccacgct ctcgacggta
    54841 aacagtcggg aatgggctac agtttcaaca ttcagaatgg tgggtttttg taatgatttg
    54901 ctcatcgtgg gatctatgct gtgaaatcag cggttattgt gcgatatcgg acacgctttc
    54961 ggcaatgtga attgcatgtt atttacattt atgtaactta ataaataatt gtcctcaaat
    55021 caaattaaaa gtcaataggt tgaaataact ccaggaattt gctgatatcc cgccttcaga
    55081 gggtttgcta tgatcagcgg ttactgtgat gtgcttaatg atgctcaagt taaactccac
    55141 gcttgccgat agccaaccgc agaatcatgt attgtgtctg gtgcgactga ccacgcctga
    55201 cagactaagt aagatgggga aagcatgagc accattgtga tttttttagc tgctttgctg
    55261 gcctgctcac tacttgcggg atggctgata aaagtgcgat ccagacggcg tcagctgccc
    55321 tggaccaacg ccttcgcgga tgcgcaaacg cgtaaactca cacctgaaga acgtagcgcc
    55381 gttgaaaatt atcttgagag cctgacgcag gtattacagg tgcctggccc aacgggagct
    55441 agcgcggcac cgatctctct ggcgctgaat gccgaaagca acaacgtcat gatgctgaca
    55501 cacgctatca cgcgttacgg catctctacc gacgatccga ataaatggcg ttactacctc
    55561 gattcggtag aagtccacct gccccctttc tgggaacaat acatcaacga tgagaatacc
    55621 gttgaactga tttataccga ttcgctgccg ctggttattt cactcaacgg tcatacgctg
    55681 caggagtaca tgcaggaaac gcgcggctat gccttgcaac ctgttccgtc cacgcaggcg
    55741 tcgattcgcg gggaagagag tgagcaaatc gagctactca atattcgcaa agaaacgcat
    55801 gaagaatatg cgctgagtcg tccgcgcggg ctgcgtgaag cgttgctgat cgtcgcctcc
    55861 ttcctgatgt tctttttctg cctgattacc ccggatgtat ttgttccgtg gctggcaggc
    55921 ggcgcattac tgctgctggg cgcaggtctg tgggggctat tcgcgccccc ggcaaaatcc
    55981 tccctgcggg aaattcattg tctgcgcggt acaccccgtc gttggggatt gtttggcgaa
    56041 aacgatcagg aacagatcaa caatatttca ctcggtatta tcgacctggt ctatcccgca
    56101 cactggcagc catacattgc tcaggatctc ggtcaacaaa ccgatattga tatctatctc
    56161 gaccgccacg tagtgcgtca ggggcgatat ctttcgctgc atgatgaagt aaaaaacttt
    56221 ccgttacagc actggctgcg cagtacgatt atcgctgcgg gctcgctgct ggtgctgttt
    56281 atgctgttat tctggatccc gctggatatg ccgctgaaat tcactctctc atggatgaaa
    56341 ggcgcacaga ccattgaagc caccagcgta aaacaactgg ctgacgctgg cgtgcgggta
    56401 ggcgatacat tgcgtatcag cggtacggga atgtgtaata ttcgcacttc cggaacctgg
    56461 agcgcgaaaa ccaattcacc ttttttaccg tttgactgct cgcagatcat ctggaacgac
    56521 gcccgctcat tgccattacc agaatctgaa ctggtcaaca aagcgacggc attgactgaa
    56581 gcagttaatc gccagctgca ccctaaaccg gaagatggat ctcgcgtcag tgcctcatta
    56641 cgttcagcaa ttcaaaaatc cggcatggta ttgcttgatg attttggcga cattgtactg
    56701 aagacagcgg atttatgttc tgccaaagat gactgtgtgc gactgaaaaa tgcgctggtc
    56761 aatctcggca acagtaaaga ctgggacgcg ctggtaaaac gcgccaacgc cgggaagctc
    56821 gatggcgtga atgtgttatt acgcccggtg agtgcggaat cgctggataa cctggtggca
    56881 acctccaccg cgccgtttat cacgcatgaa acggcgcgag cggcacaatc actaaacagt
    56941 ccggcccccg gcggattcct gattgtcagc gacgaaggca gcgattttgt tgatcagccc
    57001 tggccttcgg catcacttta cgactacccg ccgcaagaac agtggaacgc tttccagaaa
    57061 ctggcacaaa tgctgatgca tacgccgttt aacgccgagg gtatcgtcac aaaaatcttc
    57121 actgacgcca atggtacgca gcatattggc cttcatccaa tcccggatcg ttccggcctg
    57181 tggcgctatc tcagcaccac attgctgcta ctgacgatgc tgggtagcgc catttacaat
    57241 ggcgtacagg cctggcgtcg ttaccagcgt catcgcactc gcatgatgaa gattcaggcc
    57301 tattatgaaa gctgcctgaa cccgcaactg atcacccctt cagaaagcct tatcgaataa
    57361 cacgtttgcg cggcaggtta tgctaccctg tcgcgcaaat tgcttcactc tggagatttc
    57421 cctcatgcat atcaacattg cctggcagga cgtagatacc gttctgctgg atatggacgg
    57481 cacgttgctc gacctcgcct tcgataacta tttctggcaa aagctggtgc ctgaaacatg
    57541 gggcgcgaaa aacggggtta cgccacagga agcgatggaa tatatgcgcc agcaatatca
    57601 cgacgtacag catacgctaa actggtactg tcttgattac tggagtgagc aactgggtct
    57661 ggatatctgt gcgatgacca ccgagatggg accgcgtgcc gtactgcgtg aagataccat
    57721 tccgtttctt gaggcactga aagccagcgg taagcagcga attttgctca ccaatgcgca
    57781 tccgcacaac ctggcggtaa aacttgagca taccggtctg gacgcacacc ttgatttatt
    57841 actttccacc cacacatttg gttatccgaa agaggatcag cggttatggc atgcggtggc
    57901 cgaagctacg ggtctgaaag ctgaaagaac gctgtttatt gatgacagcg aagcgattct
    57961 cgatgctgcc gcgcaatttg gtattcgtta ctgcctcggc gtgactaatc ctgattccgg
    58021 gattgccgag aaacagtatc aacgccatcc gtcactgaat gactaccgcc gcctgatccc
    58081 ctcgctaatg tgaaggagac gccatgaaag aaaaacctgc tgttgaggtt cgactggata
    58141 aatggctatg ggctgcccgt ttttataaaa cccgcgcgct ggcccgtgaa atgattgaag
    58201 gcggtaaggt gcattacaac gggcagcgca gcaagccgag caaaatcgtc gagctgaatg
    58261 ccacgctcac tctgcgccag ggaaatgacg aacgcacggt gattgtaaag gcgattactg
    58321 aacagcgtcg ccccgccagc gaggcagcct tgctgtatga agagactgcg gaaagtgtag
    58381 agaaacgcga aaaaatggcg ctggcacgta aacttaatgc cttaaccatg ccgcacccgg
    58441 accgacgccc ggacaaaaaa gagcgccgcg acctgttacg atttaaacac ggcgacagtg
    58501 aataactgtc acctgcaaga gagatgatta tgccgcaaca tgaccaatta catcgctatc
    58561 tgtttgaaaa ctttgccgtg cgcggcgaac tggtaaccgt ttcggaaacc ctgcaacaga
    58621 tccttgagaa tcacgattat ccgcagcccg ttaaaaacgt gctggcagaa ctgctggttg
    58681 cgaccagcct gttaaccgct acgctgaagt ttgatggtga tatcaccgta cagctgcaag
    58741 gcgacggtcc gatgaatctg gcggttatta acggtaacaa taaccagcaa atgcgcggtg
    58801 tggcgcgcgt gcagggcgaa attccagaaa atgccgacct gaaaacgctg gtcggcaatg
    58861 gttacgtggt gatcaccatt accccgagcg aaggcgaacg ctatcagggc gtggttggtc
    58921 tggaaggtga taccctggcg gcctgcctgg aagattactt tatgcgttct gaacagctgc
    58981 cgacgcgcct gtttattcgc accggcgacg tagacggcaa accggctgca ggcggtatgt
    59041 tgttgcaggt aatgcctgcg caaaatgccc agcaggacga ctttgaccac ctggcgacgc
    59101 taaccgaaac catcaaaacc gaagaactgc tgaccttacc ggcaaacgaa gtgttgtggc
    59161 gtttgtatca cgaagaagag gtgacggttt acgatccgca ggatgtggag ttcaaatgca
    59221 cctgctcgcg tgaacgttgc gccgacgcgc tgaaaacgct gcctgatgaa gaagtcgata
    59281 gcatcctggc ggaagatggc gaaattgaca tgcattgtga ttactgcggt aaccactatc
    59341 tgttcaatgc gatggatatt gctgaaatcc gcaacaacgc gtctccggca gatccgcaag
    59401 ttcattaatg agtatgtccg gcagagaggg ctctgccgga ttttacgacg taatggcctg
    59461 atgtgctgcg cttaccaggc ctgtgaaata aatcgcaaat tattgttggt cagatacagc
    59521 gtcgtatccg ttaaaagagt tgtcacttac ttaagtttat ttccagcgtt tcaccatcta
    59581 ccgtcagatc cagttttcca tccttcgcag ctgtgagtaa tttttctttc gttatttcgt
    59641 tcggaagtaa actcatatca agcgcgtccc attcttttct gtctgagtca aagccataga
    59701 caatgactct gtcatcatta aaagcaaaca ggatccgctc cgcctgacca tcactattca
    59761 gatcttgctc gaccaggacg caggcgtctt tttcaataca ggtcatcacg ttatagcgat
    59821 cctggattaa ggccgaccag aatgtcgcat ccggtttaac agaaccagga gcaattaaca
    59881 cattttcggc taatactttt tccgatacct gttcctgaag atgttgctct ccatctaacg
    59941 ccatcagcag atcccgcgcg cgtttcgggt ctttcataaa cccggcatcg cttttcagcg
    60001 actcaagcgc cgcacgtcca tagcgaccgc tctgttcgag catgtagata gttacctggt
    60061 ctggcgtgtt tttgccgctc tgataacgcg ccatatgact gttcacgcta atgcgcatac
    60121 tgtccagcac cggcgaatta agaagcacca gtatcaccaa caccaataac gaaaccgcaa
    60181 ggttcacttt gccctgaaga acaaggggat tttgcccatt acgccagacg atactgacaa
    60241 aataccccag cgaccacacc agtaacacca gcaccgccag cgcgccctgc aagcggtcga
    60301 cagtccagcc gtactgagcg acccgcagcc ataacgccca ggcggcgacg aacacataca
    60361 gcggagcaac cagcaaagcg gttttaatca ggcaacgcaa cggccccgtc cagggaagtg
    60421 acgctttttg cggatcgcgg acaatagcca ttaagatcaa ttgcaaaaag gccagcgtta
    60481 acagcaaccc ggcggcggag atgtggcgag aaatcgcgct caggtccgta aacggcaggg
    60541 tgatgataaa catcagggtt agcaatgata ctaacggcag caaccccgtg gcgattagcg
    60601 tgaacaactt ttgaatagag tcgattaaac gtgactgtgt acgcgcgagg atcaccacca
    60661 gtgcggtaac cagaccaaat gtaagataca taaaccagtc ggttgcaaaa aagagcgtat
    60721 tgaaaaacgt gatgccaaca agtttaaaca actcacccca cagtaaaagc accagccacg
    60781 tcaggccatt ggagagaaaa atcaccagta atataagtac gttatgccat actgactggt
    60841 aaaagtagct atagcggcta ctgccattgc ggatacgaag gctttgttgt atccacggta
    60901 gcaacaacat tgccatcaac agcagatatc agccaaaatc ccaaatcgcc ttttcagctc
    60961 tccaggggtt catgccatca gtttgccact tcagccaacc gctcatccct gtcgtggcaa
    61021 taaacaccag cgccagccat ccccaaaggc gcttctgttt aaaagagatc acggagaaga
    61081 gtaaaacgga tgaaaacgcg acggtcgcag gcacgccata aacaatccaa ctgtgatttt
    61141 ttccggcaag ccaggcgata agcaggtagc aaaccagccc ctgaagtaat ccggtagcaa
    61201 tcatccccca gcgtgttgcc ggtgaaagtt caacgttatc catgttggtt atccagaatc
    61261 aaaaggtggg ttaattatcg catccgggca gtagtatttt gcttttttca gaaaataatc
    61321 aaaaaaagtt agcgtggtga atcgatactt taccggttga atttgcatca atttcattca
    61381 ggaatgcgat tccactcaca atattcccgc catataaacc aagatttaac cttttgagaa
    61441 cattttccac acctaaaatg ctatttctgc gataatagca accgtttcgt gacaggaatc
    61501 acggagtttt ttgtcaaata tgaatttctc cagatacgta aatctatgag ccttgtcgcg
    61561 gttaacaccc ccaaaaagac tttactattc aggcaataca tattggctaa ggagcagtga
    61621 aatgcgcgtt aacaatggtt tgaccccgca agaactcgag gcttatggta tcagtgacgt
    61681 acatgatatc gtttacaacc caagctacga cctgctgtat caggaagagc tcgatccgag
    61741 cctgacaggt tatgagcgcg gggtgttaac taatctgggt gccgttgccg tcgataccgg
    61801 gatcttcacc ggtcgttcac caaaagataa gtatatcgtc cgtgacgata ccactcgcga
    61861 tactttctgg tgggcagaca aaggcaaagg taagaacgac aacaaacctc tctctccgga
    61921 aacctggcag catctgaaag gcctggtgac caggcagctt tccggcaaac gtctgttcgt
    61981 tgtcgacgct ttctgtggtg cgaacccgga tactcgtctt tccgtccgtt tcatcaccga
    62041 agtggcctgg caggcgcatt ttgtcaaaaa catgtttatt cgcccgagcg atgaagagct
    62101 ggcaggtttc aaactagact ttatcgttat gaacggcgcg aagtgcacta acccgcagtg
    62161 gaaagaacag ggtctgaact ccgaaaactt cgtggcgttt aacctgaccg agcgcatgca
    62221 gctgattggc ggcacctggt acggcggcga aatgaagaaa gggatgttct cgatgatgaa
    62281 ctacctgctg ccgctgaaag gtatcgcttc tatgcactgc tccgccaacg ttggcgagaa
    62341 aggcgatgtt gcggtgttct tcggcctttc cggcaccggt aaaaccaccc tttccaccga
    62401 cccgaaacgc cgcctgattg gcgatgacga acacggctgg gacgatgacg gcgtgtttaa
    62461 tttcgaaggc ggctgctacg caaaaactat caagctgtcg aaagaagcgg aacctgaaat
    62521 ctacaacgct atccgtcgtg atgcgctgct ggaaaacgtc accgtgcgtg aagatggcac
    62581 tatcgacttt gatgatggtt caaaaaccga gaacacccgc gtttcttatc caatctatca
    62641 catcgataac atcgttaagc cggtttccaa agcgggccac gcgactaagg ttatcttcct
    62701 gactgctgat gctttcggcg tgttgccgcc ggtttctcgc ctgactgccg atcaaaccca
    62761 gtatcacttc ctctctggct tcaccgccaa actggccggt actgagcgtg gcatcaccga
    62821 accgacgcca accttctccg cttgcttcgg cgcggcgttc ctgtcgctgc acccgactca
    62881 gtacgcagaa gtgctggtga aacgtatgca ggcggcgggc gcgcaggctt atctggttaa
    62941 cactggctgg aacggcactg gcaaacgtat ctcgattaaa gatacccgcg ccattatcga
    63001 cgccatcctc aacggttcgc tggataatgc agaaaccttc actctgccga tgtttaacct
    63061 ggcgatccca accgaactgc cgggcgtaga cacgaagatt ctcgatccgc gtaacaccta
    63121 cgcttctccg gaacagtggc aggaaaaagc cgaaaccctg gcgaaactgt ttatcgacaa
    63181 cttcgataaa tacaccgaca cccctgcggg tgccgcgctg gtagcggctg gtccgaaact
    63241 gtaatgattt gaagctggag aatatctatc cagtatctta tagaaagcaa aacgggaggc
    63301 accttcgcct cccgtttatt tacccttctt ttgtcgtgcc ctgcgcccgc gttaccggca
    63361 ctggcagcca ggcgcgaatg gaaagcccgc cccgctcgct ggtgccaagc tccagcatcc
    63421 cgttatggtt atccacgata cgctgcacaa ttgccagccc taatcccgtg ccgctaatgg
    63481 tgcgcgcact gtcgccgcgg acaaacggct ggaacaggtg cttacgttgt tccggcgcaa
    63541 ttcccggacc gtcatcttcc acctggaacc aggcgcgatt cggctccgtt ccgctgctga
    63601 ctttgatcca gccattgcca taacgagcgg cgttgaccac catattcgcc accgcgcgtt
    63661 tgatcgacag cgggtgcatt ttcacttcaa tgctgccggg gtaaagcgcg gtttcaattt
    63721 cccgctcata gccactttcg gcagcaatca cctcaccgag cactgcatta agatccgcca
    63781 tttccatcgg catctcctgc ccggtgcgca ggtagtcgat aaactgctca atgatggcgt
    63841 tgcactcttc gatatcttta ttgatcgatt ctgccagata gccatcctgc tcgctcatca
    63901 tctcagtcgc caggcgaata cgcgtcagcg gcgtgcgcaa gtcgtgactt acccccgcca
    63961 tcagcagcgt gcggtcatcc gccagttgct taacaccagc cgccatatgg ttaaaggcgc
    64021 gggtaacgga acgcacctcg gacgcgccat actcacgcag cggcggcgga ataatccctt
    64081 taccgacctg caaagcagca tgttcgagat cgaccaacgg tcggttctgg atacgaataa
    64141 acagccacgc cccgcctatc gccaatagca taatcgccag cgtatagcgg aacagcggag
    64201 agaaatcgcc ctgatgaatt tcggtcagcg gcacgcgtac ccagatattg ggcgacagcc
    64261 aggttttcag ccagacgaca ggcgaacttt tgttgacctc aacgcgcact tccgtcgggc
    64321 cgcccagttg ctgtgccatc tgatggctta agaattcata gtgttgcgcc caacgcagac
    64381 ctgcctcttc ggcagcctcg ttggagtaga gagagatccc cagctcacgg tagatctccc
    64441 gacggaaagc gggaggcaca accaactgcg tgccgtcctc cagttgcagt ttgtcggtca
    64501 tcaacatacg cacttcgtac gcgaggactt tattaaactg ctggaggctc ggcaaaatcg
    64561 cgaagttcag caccaccaga taagtcgtca ccaggctggc gaacagcaag gtgacgatga
    64621 gcaataacgt acgggcaaat gaacttcgtg gcgagaagcg caatcgcctc atgctttaga
    64681 gccgtccggt acaaagacgt agcccaggcc ccaaacggtc tgaatgtaac gcggatgcgc
    64741 tggatcttct tctaccatgc ggcgcagacg cgaaatctgc acgtcgatgg agcgttccat
    64801 tgcggaatat tcacgaccac gggcaaggtt catcagctta tcgcgggaga gcggctcacg
    64861 cggatggctg accagtgcct tcagtaccgc aaactcaccg ctggtgagcg gcatcggctc
    64921 atcttcgcgg aacatttcgc gcgtaccgag gttaagtttg aacttaccga aagcaattac
    64981 cgcctcttcc tgtgacggtg cgcctggcag ttcgttcgcc tgacgacgca gcaccgcacg
    65041 aatacgggcc agcagttcac gcgggttaaa cggttttgga atgtagtcgt cagcgccaat
    65101 ctccaggcct acgatacggt ccacttcttc ccctttcgcc gtcaccataa tgatcggcat
    65161 cgggttgctc tgactacgaa gacgtcggca aatcgacaag ccatcttcac caggtaacat
    65221 taaatccagt accataagat ggaaagattc acgagtcagc aggcgatcca tctgttctgc
    65281 attagcgacg cttcgaacct ggaagccttg ttcggtgaga taacgttcca gcagcgcacg
    65341 caggcgcatg tcgtcatcga ccaccagaat cttgtagttc tcttgcattg tttgtactcc
    65401 caaaggttcg caacaatttg taagcgtgta ttcttaaaaa agctcacgtt cgtcaccagc
    65461 taaatctggt atgaatttca gcctaaattg ttacaaagca tattaaacag cagcttaagt
    65521 atacaattta ttcggcgaaa cattattgat tctgttgata tgatcacgtt atacccaatg
    65581 tgcgcattat caaacagaca aagggaatca acgagatgaa aacgcccctg gttacccggg
    65641 aagggtatga aaaactcaaa caagagctta attatctctg gcgtgaagaa cgcccggagg
    65701 tcacaaaaaa ggtgacctgg gccgcaagtc tgggcgaccg cagcgaaaat gctgactatc
    65761 agtataataa aaagcgtctg cgtgaaatcg accgtcgcgt gcgctatctc actaaatgca
    65821 tggaaaatct caaaatcgtc aattactccc ctcagcagga aggcaaagtc ttttttggcg
    65881 cgtgggtgga gattgaaaac gacgatggcg tgactcaccg tttccgtatt gtcggctacg
    65941 atgaaatttt tggccgtaaa gattacatct ctatcgattc cccgatggcc cgcgcattgc
    66001 tgaaaaaaga agtcggcgat ctggcggtgg tgaatacccc tgccggggaa gcgagctggt
    66061 atgttaatgc tatcgaatac gtgaaaccgt aatgaagagt cttaacctcc tgccgatggc
    66121 tggcattttt gccagccagt ccgtataact atcccctgat ttttgatccg aaaagatgaa
    66181 ctcaaaccat gatgaatgat tcgttctgcc gcattattgc gggtgaaatt caggcgcgcc
    66241 cggaacaggt tgacgctgcc gttcgcctgc ttgacgaagg gaataccgtg ccgtttatcg
    66301 cacgttatcg taaggaaatc accggcggtc tggatgacac gcagctgcgt aatctggaaa
    66361 cgcgtctgag ctatctgcgc gagctggaag agagacgtca ggcgatcctc aagtccattt
    66421 ccgagcaagg caaactcacc gatgatctgg cgaaggccat caacgccacc ctaagcaaaa
    66481 ccgaactcga agacctctac ctgccctaca aacctaaacg ccgcacccgc gggcaaatcg
    66541 ccattgaagc agggcttgag ccgttggctg acctgctgtg gagcgatccg tcacacacac
    66601 cagaagtcgc cgctgcgcaa tatattgatg ccgataaagg cgtggcagat accaaagccg
    66661 cgctggacgg cgcgcgctat atcctgatgg aacggtttgc cgaagatgcc gcgctgctgg
    66721 cgaaagtgcg tgattatctg tggaagaacg cgcatttggt ttctacggtg gtgagcggta
    66781 aagaagagga aggggcgaaa ttccgcgact atttcgatca tcacgaaccg ttgtccacgg
    66841 tgccttctca ccgcgcgctg gcgatgttcc gtgggcgtaa cgaaggcgta ctccagcttt
    66901 cgctgaatgc cgatccgcag ttcgatgagc cgcccaaaga gagctattgc gagcaaatca
    66961 tcatggatca ccttggcctg cgcctgaaca atgccccggc ggatagctgg cgcaaaggcg
    67021 tagtgagctg gacctggcgc atcaaggtgc tgatgcatct ggaaaccgaa ctgatgggta
    67081 ccgtgcgcga acgcgcggaa gatgaagcaa tcaacgtctt tgcccgtaac ctgcacgatc
    67141 tgctgatggc ggcccctgcc ggactgcgtg caacgatggg cctcgatccg ggtctgcgta
    67201 ccggggtaaa agtggcagtg gtcgatgcca ctggcaaact ggtggcgacc gataccattt
    67261 acccgcacac cggacaagcc gcaaaagtag cgatgaccgt tgctgccttg tgtgaaaaac
    67321 ataacgttga actggtggcg atcggcaacg gtacagcttc ccgcgaaact gaacgtttct
    67381 atctcgacgt gcagaagcag ttcccgaaag tgaccgcaca gaaagtgatc gtcagcgaag
    67441 ctggcgcgtc ggtttactca gcttccgagc tggcagcgca ggagttcccg gatctcgacg
    67501 tttcgctgcg tggtgcggta tctatcgccc gccgtttgca ggatccgctg gcggagctgg
    67561 tgaaaatcga tccgaaatct atcggcgtag gtcagtatca gcatgacgtc agccagacgc
    67621 aactggcccg caaactggat gcagtagtag aagactgcgt aaacgccgtt ggcgtcgatc
    67681 tcaacaccgc ttctgttccg ctattaactc gcgtggcggg cctgacgcgc atgatggcgc
    67741 aaaacatcgt ggcctggcgc gatgagaacg gccagttcaa gaaccgtcag caactgctga
    67801 aagtcagccg tctggggccg aaagccttcg agcagtgcgc gggcttcctg cgcattaacc
    67861 acggtgataa cccgctggac gcctctaccg ttcacccgga agcctatccg gtggtggaac
    67921 gcattctggc agcaacacag caggcactga aagatctgat gggtaacagc agcgaactgc
    67981 gtaacctgaa agcgtctgac tttactgatg aaaaattcgg tgtgccgaca gtaactgaca
    68041 tcatcaaaga gctggaaaaa ccgggtcgcg atccgcgtcc ggaatttaaa accgctcagt
    68101 ttgccgatgg cgtcgagaca atgaacgacc tgcaaccggg tatgatcctc gaaggcgctg
    68161 tcaccaacgt caccaacttt ggcgcgtttg tcgatattgg cgtgcatcag gacggcctgg
    68221 ttcacatctc ttcattgtcg aacaagtttg tggaagatcc gcataccgtg gtgaaagcgg
    68281 gcgacattgt gaaggtgaaa gtgctggaag tggatcttca gcgtaaacgt atcgccctga
    68341 ccatgcgtct ggacgagcag cctggcgaaa ccaacgcccg tcgcggcggc ggtaatgaac
    68401 gcccgcaaaa caaccgcccg gcagccaaac cacgcggtcg tgaagcgcag cctgccggta
    68461 atagcgcgat gatggatgcg ctggcggcgg caatgggcaa aaaacgttaa acgcccggac
    68521 tggcccctaa acctgcgcga taaggcgttc acgccgcatc tggcaacgaa cgccttgcct
    68581 gacataaaag tgccggagaa tatctccggc ctttttattc cacaaccaaa ctcataatat
    68641 attccggcaa tatttatcac ttcattaaca actgaaacct taattaaaca ttagccagtc
    68701 cgggtaattc actattcgaa ttatattttc gctgcgatat aaccttgagc cacatcaaca
    68761 ttgagtcaga ttattattca aaccaacatt cgcacacatt ttaagtattg ctgatagaaa
    68821 ccattctcat tatcattgtg ttgttgatta tttaatctct cctttgttgg caaatcatct
    68881 ggtctcatgt cgctgtcaaa cgccccatga ggtagttatc cagttaatga gaaacaagta
    68941 ggcacctatg caatacactc cagatactgc gtggaaaatc actggctttt cccgtgaaat
    69001 cagcccggca tatcgccaaa aactgctttc tcttggcatg ttacctggct cctcttttaa
    69061 tgtggtgcgc gtcgctccac tcggcgaccc cattcatatc gaaacccgtc gtgtgagcct
    69121 ggtattacgc aaaaaagatc tggccttatt agaagtggaa gcggtttcct gttaatacgg
    69181 tgataacaac aatgaaaaaa ttaaccattg gcttaattgg taatccaaat tctggcaaga
    69241 caaccttatt taaccagctc actagcgcac gtcagcgtgt aggtaactgg gctggcgtta
    69301 ccgtcgaacg taaagaaggg caattctcca ccaccgatca tcaggtcacg ctggtggacc
    69361 tgcccggcac ctattctctg accaccatct catcgcagac ctcgctcgat gagcaaatcg
    69421 cctgtcacta cattttgagt ggcgacgccg acctgctgat taacgtggtg gatgcgtcta
    69481 accttgagcg taacctgtac ctgacgctac aactgctgga actcggcatt ccctgcatcg
    69541 tggcactgaa catgctcgac attgccgaga agcaaaatat tcgtattgaa attgatgctc
    69601 tgtcggcgcg tcttggctgc ccagtgatcc cgctggtttc aacccgtggt cgcggtattg
    69661 aagcgctcaa gctggcgatt gatcgctata aagctaacga gaatgtggaa ctggtgcatt
    69721 acgcacagcc gctactcaac gaagcagatt cactggcaaa agtgatgcct tccgacatcc
    69781 cgctgaaaca acgtcgctgg ctgggcctgc aaatgctgga aggcgatatc tacagccgcg
    69841 cctacgccgg tgaagcgtcg cagcatctgg atgccgccct tgcccgtctg cgtaatgaaa
    69901 tggatgatcc ggcgctgcac attgccgatg cgcgttacca gtgcatagct gccatctgtg
    69961 atgtggtaag caacaccctg acggcagaac ccagccgttt caccactgcg gtagataaaa
    70021 tcgtgctcaa ccgtttcctc ggtctgccga ttttcctctt tgtgatgtac ctgatgttcc
    70081 tgctggctat caacatcggc ggggcgttac agccgctgtt tgacgtcggc tccgtggcgc
    70141 tatttgtgca tggtattcaa tggattggct acacgctcca cttcccggac tggctgacta
    70201 tcttcctcgc ccagggcctg ggtggcggta ttaacaccgt gctgccactg gtgccgcaga
    70261 tcggcatgat gtacctgttc ctctccttcc ttgaggactc cggctatatg gcgcgtgcag
    70321 cgtttgtgat ggaccgcctg atgcaggcgc tgggcttgcc ggggaaatcc tttgtgccgc
    70381 tgatcgtcgg tttcggttgt aacgtaccgt cggtaatggg cgcacgtacg cttgatgcac
    70441 cgcgtgaacg tctgatgacc atcatgatgg caccgtttat gtcctgcggc gcgcgtctgg
    70501 ctatcttcgc agtattcgcg gctgccttct tcgggcagaa cggtgccctg gcggttttct
    70561 cgctgtatat gctcggtatc gtgatggcgg tgctgactgg cctgatgctc aagtacacca
    70621 tcatgcgcgg tgaagcgacg ccgtttgtca tggagctgcc ggtctaccat gtaccacacg
    70681 ttaaaagcct gattatccag acctggcagc gtctgaaggg cttcgttctg cgtgctggta
    70741 aagtgattat catcgtcagc attttcctga gcgctttcaa cagcttctcg ttgagcggga
    70801 aaatcgtcga taacatcaac gactcggcgc tggcgtccgt cagccgggtg ataacccctg
    70861 tcttcaagcc gattggcgta catgaagata actggcaggc aacggttggc ctgtttacgg
    70921 gcgcaatggc aaaagaagtg gtggtgggta cgctcaacac cctctacacc gcagaaaata
    70981 ttcaggacga agagttcaat ccggcggaat tcaacctcgg tgaagagctg ttcagtgcgg
    71041 tagatgaaac ctggcagagc ctgaaagaca ccttcagcct tagcgtactg atgaacccca
    71101 ttgaagctag caaaggcgac ggtgaaatgg gtaccggggc gatgggcgtg atggatcaga
    71161 aattcggtag cgcagctgcc gcatacagct acctgatttt cgtcctgctg tatgtaccat
    71221 gtatctcggt gatgggagcc atcgcccgtg aatcaagccg tggctggatg ggcttctcca
    71281 tcctgtgggg gctgaatatc gcttactcac tggcaacatt gttctatcag gtcgccagct
    71341 acagtcagca cccaacttac agcctggtgt gcattctggc ggtaatcctg tttaacatcg
    71401 tagttatcgg tctgctgcgc cgcgcgcgta gccgggtgga tatcgaactg ctggcaaccc
    71461 gcaagtcggt aagcagttgc tgcgcagcca gcaccaccgg tgattgccat taatggcttc
    71521 acttattcag gtacgtgatt tgctggcgtt acggggccgt atggaagcga cccagataag
    71581 ccagacattg aacactccgc agccaatgat taacgccatg ctgcaacaac tggaaagtat
    71641 gggcaaagcc gtgcggattc aggaagaacc tgacggctgc ctctctggca gttgtaaaag
    71701 ctgcccggaa ggaaaagcct gtctgcgcga gtggtgggcg ctgcgttaac cttactccat
    71761 cgcctgtttt ggaaagcccg gtatgcgtct gcatccgggc ttttttgcgt gcggctttcc
    71821 ataaaaatgc aactcttgca gcacggcgta agttcctttg aaagcatctc gcagggatga
    71881 aaactcgcta atgcacaggt gtggagtggc gcgtagagtc gcggcattca aacaacaggt
    71941 gaaggaacgc catgagcaaa aagcagagtt ccaccccaca cgatgcgctg ttcaaactct
    72001 ttttacgcca accggacacg gctcgtgatt ttcttgcgtt tcatttaccg gcacccattc
    72061 acgcgctttg tgatatgaaa accctcaagc tggagtcgag cagctttatt gatgacgatc
    72121 tgcgtgaaag ctattccgat gtgctgtggt cggtgaaaac ggaacaagga ccaggataca
    72181 tctattgtct gattgaacat caaagcacct caaacaaact gatcgcattt cgcatgatgc
    72241 gttacgctat tgccgcaatg caaaatcacc ttgatgctgg atacaaaacg ttgccgatgg
    72301 tggtgctatt gttgttttac cacggtattg aaagccccta tccctattcg ctgtgttggc
    72361 tggattgttt cgccgatccc aaactggcaa ggcagcttta tgcctccgca tttccgctga
    72421 ttgatgtcac cgtcatgcct gatgatgaaa tcatgcagca ccgacgtatg gcgctgctgg
    72481 agttaattca aaaacatatt cgtcaacgcg
//
LOCUS       DAESHI010000005        70303 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.005, whole genome shotgun sequence.
ACCESSION   DAESHI010000005 DAESHI010000000
VERSION     DAESHI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70303
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.005"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..674)
                     /locus_tag="KW011_000333"
     CDS             complement(<1..674)
                     /locus_tag="KW011_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HBH7658773.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRH"
     gene            complement(696..2708)
                     /gene="hyfR"
                     /locus_tag="KW011_000334"
     CDS             complement(696..2708)
                     /gene="hyfR"
                     /locus_tag="KW011_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HBH7658774.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSHLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGLSVREVL"
     gene            complement(2738..3151)
                     /gene="hyfJ"
                     /locus_tag="KW011_000335"
     CDS             complement(2738..3151)
                     /gene="hyfJ"
                     /locus_tag="KW011_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HBH7658775.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3144..3902)
                     /gene="hyfI"
                     /locus_tag="KW011_000336"
     CDS             complement(3144..3902)
                     /gene="hyfI"
                     /locus_tag="KW011_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HBH7658776.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3899..4438)
                     /gene="hyfH"
                     /locus_tag="KW011_000337"
     CDS             complement(3899..4438)
                     /gene="hyfH"
                     /locus_tag="KW011_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HBH7658777.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4448..6163)
                     /gene="hyfG"
                     /locus_tag="KW011_000338"
     CDS             complement(4448..6163)
                     /gene="hyfG"
                     /locus_tag="KW011_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HBH7658778.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6153..7733)
                     /gene="hyfF"
                     /locus_tag="KW011_000339"
     CDS             complement(6153..7733)
                     /gene="hyfF"
                     /locus_tag="KW011_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HBH7658779.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7738..8388)
                     /gene="hyfE"
                     /locus_tag="KW011_000340"
     CDS             complement(7738..8388)
                     /gene="hyfE"
                     /locus_tag="KW011_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HBH7658780.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8400..9752)
                     /gene="hyfD"
                     /locus_tag="KW011_000341"
     CDS             complement(8400..9752)
                     /gene="hyfD"
                     /locus_tag="KW011_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HBH7658781.1"
                     /translation="MGVLFAALTTLCMLSLISAFYQADKVAVTLTLVNVGDVALFGLV
                     IDRMSTLILFVVVFLGLLVTIYSTGYLTDKNREHPHNGTNRYYAFLLVFIGAMAGLVL
                     SSTLLGQLLFFEITGGCSWALISYYQSDKAQLSALKALLITHIGSLGLYLAAATLFLQ
                     TGTFALSAMSELHGDARYLVYGGILFAAWGKSAQLPMQAWLPDAMEAPTPISAYLHAA
                     SMVKVGVYIFARAIIDGGNIPHVIGGVGMVMALVTILYGFLMYLPQQDMKRLLAWSTI
                     TQLGWMFFGLSLSIFGSRLALEGSIAYIVNHAFAKSLFFLVAGALSYSCGTRLLPRLR
                     GVLHTLPLPSVGFCVAALAITGVPPFNGFFSKFPLFAVGFALSVEYWILLPAMILLMI
                     ESVASFAWFIRWFGRVVPGKPSEAVADAAPLPGSMRLVLIVLIVMSLISSVIAATWLQ
                     "
     gene            complement(9728..9838)
                     /locus_tag="KW011_000342"
     CDS             complement(9728..9838)
                     /locus_tag="KW011_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HBH7658782.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(9855..10802)
                     /gene="hyfC"
                     /locus_tag="KW011_000343"
     CDS             complement(9855..10802)
                     /gene="hyfC"
                     /locus_tag="KW011_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HBH7658783.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10813..12831)
                     /gene="hyfB"
                     /locus_tag="KW011_000344"
     CDS             complement(10813..12831)
                     /gene="hyfB"
                     /locus_tag="KW011_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HBH7658784.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVVCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12831..13448)
                     /gene="hyfA"
                     /locus_tag="KW011_000345"
     CDS             complement(12831..13448)
                     /gene="hyfA"
                     /locus_tag="KW011_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HBH7658785.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13701..14171)
                     /gene="bcp"
                     /locus_tag="KW011_000346"
     CDS             complement(13701..14171)
                     /gene="bcp"
                     /locus_tag="KW011_000346"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBH7658786.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14171..14743)
                     /locus_tag="KW011_000347"
     CDS             complement(14171..14743)
                     /locus_tag="KW011_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HBH7658787.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14889..15767
                     /gene="dapA"
                     /locus_tag="KW011_000348"
     CDS             14889..15767
                     /gene="dapA"
                     /locus_tag="KW011_000348"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBH7658788.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15784..16818
                     /gene="bamC"
                     /locus_tag="KW011_000349"
     CDS             15784..16818
                     /gene="bamC"
                     /locus_tag="KW011_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HBH7658789.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17031..17744
                     /gene="purC"
                     /locus_tag="KW011_000350"
     CDS             17031..17744
                     /gene="purC"
                     /locus_tag="KW011_000350"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBH7658790.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17912..18760
                     /locus_tag="KW011_000351"
     CDS             17912..18760
                     /locus_tag="KW011_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HBH7658791.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18775..20790
                     /gene="tmcA"
                     /locus_tag="KW011_000352"
     CDS             18775..20790
                     /gene="tmcA"
                     /locus_tag="KW011_000352"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HBH7658792.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRGSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20864..21562
                     /gene="ypfH"
                     /locus_tag="KW011_000353"
     CDS             20864..21562
                     /gene="ypfH"
                     /locus_tag="KW011_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBH7658793.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21672..21872)
                     /locus_tag="KW011_000354"
     CDS             complement(21672..21872)
                     /locus_tag="KW011_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HBH7658794.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21900..23027)
                     /gene="dapE"
                     /locus_tag="KW011_000355"
     CDS             complement(21900..23027)
                     /gene="dapE"
                     /locus_tag="KW011_000355"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HBH7658795.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23031..23387)
                     /locus_tag="KW011_000356"
     CDS             complement(23031..23387)
                     /locus_tag="KW011_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HBH7658796.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            23769..23828
                     /gene="ypfM"
                     /locus_tag="KW011_000357"
     CDS             23769..23828
                     /gene="ypfM"
                     /locus_tag="KW011_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HBH7658797.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(23927..27040)
                     /gene="acrD"
                     /locus_tag="KW011_000358"
     CDS             complement(23927..27040)
                     /gene="acrD"
                     /locus_tag="KW011_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HBH7658798.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(27204..28904)
                     /gene="narQ"
                     /locus_tag="KW011_000359"
     CDS             complement(27204..28904)
                     /gene="narQ"
                     /locus_tag="KW011_000359"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HBH7658799.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            29110..31089
                     /gene="aegA"
                     /locus_tag="KW011_000360"
     CDS             29110..31089
                     /gene="aegA"
                     /locus_tag="KW011_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HBH7658800.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            31157..31732
                     /gene="nudK"
                     /locus_tag="KW011_000361"
                     /pseudo
     CDS             31157..31732
                     /gene="nudK"
                     /locus_tag="KW011_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
     gene            31858..32901
                     /locus_tag="KW011_000362"
     CDS             31858..32901
                     /locus_tag="KW011_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HBH7658801.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(32997..35000)
                     /gene="tkt"
                     /locus_tag="KW011_000363"
     CDS             complement(32997..35000)
                     /gene="tkt"
                     /locus_tag="KW011_000363"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBH7658802.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(35020..35970)
                     /gene="tal"
                     /locus_tag="KW011_000364"
     CDS             complement(35020..35970)
                     /gene="tal"
                     /locus_tag="KW011_000364"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBH7658803.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            36259..38538
                     /gene="maeB"
                     /locus_tag="KW011_000365"
     CDS             36259..38538
                     /gene="maeB"
                     /locus_tag="KW011_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HBH7658804.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            38832..39167
                     /gene="eutS"
                     /locus_tag="KW011_000366"
     CDS             38832..39167
                     /gene="eutS"
                     /locus_tag="KW011_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HBH7658805.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            39180..39659
                     /gene="eutP"
                     /locus_tag="KW011_000367"
     CDS             39180..39659
                     /gene="eutP"
                     /locus_tag="KW011_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HBH7658806.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            39634..40335
                     /gene="eutQ"
                     /locus_tag="KW011_000368"
     CDS             39634..40335
                     /gene="eutQ"
                     /locus_tag="KW011_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HBH7658807.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            40332..41135
                     /gene="eutT"
                     /locus_tag="KW011_000369"
     CDS             40332..41135
                     /gene="eutT"
                     /locus_tag="KW011_000369"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HBH7658808.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            41132..42148
                     /gene="pta"
                     /locus_tag="KW011_000370"
     CDS             41132..42148
                     /gene="pta"
                     /locus_tag="KW011_000370"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBH7658809.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            42187..42480
                     /gene="eutM"
                     /locus_tag="KW011_000371"
     CDS             42187..42480
                     /gene="eutM"
                     /locus_tag="KW011_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBH7658810.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            42587..42874
                     /gene="eutN"
                     /locus_tag="KW011_000372"
     CDS             42587..42874
                     /gene="eutN"
                     /locus_tag="KW011_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HBH7658811.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            42886..44289
                     /locus_tag="KW011_000373"
     CDS             42886..44289
                     /locus_tag="KW011_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HBH7658812.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            44300..45136
                     /gene="eutJ"
                     /locus_tag="KW011_000374"
     CDS             44300..45136
                     /gene="eutJ"
                     /locus_tag="KW011_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HBH7658813.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            45126..46313
                     /gene="eutG"
                     /locus_tag="KW011_000375"
     CDS             45126..46313
                     /gene="eutG"
                     /locus_tag="KW011_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HBH7658814.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            46630..47856
                     /gene="eutH"
                     /locus_tag="KW011_000376"
     CDS             46630..47856
                     /gene="eutH"
                     /locus_tag="KW011_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBH7658815.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            47853..49256
                     /gene="eutA"
                     /locus_tag="KW011_000377"
     CDS             47853..49256
                     /gene="eutA"
                     /locus_tag="KW011_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBH7658816.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            49268..50629
                     /gene="eutB"
                     /locus_tag="KW011_000378"
     CDS             49268..50629
                     /gene="eutB"
                     /locus_tag="KW011_000378"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HBH7658817.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNRSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            50650..51537
                     /gene="eutC"
                     /locus_tag="KW011_000379"
     CDS             50650..51537
                     /gene="eutC"
                     /locus_tag="KW011_000379"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HBH7658818.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            51547..52206
                     /gene="eutL"
                     /locus_tag="KW011_000380"
     CDS             51547..52206
                     /gene="eutL"
                     /locus_tag="KW011_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBH7658819.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            52219..52719
                     /gene="eutK"
                     /locus_tag="KW011_000381"
     CDS             52219..52719
                     /gene="eutK"
                     /locus_tag="KW011_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HBH7658820.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            52765..53817
                     /gene="eutR"
                     /locus_tag="KW011_000382"
     CDS             52765..53817
                     /gene="eutR"
                     /locus_tag="KW011_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HBH7658821.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(53823..54722)
                     /gene="hemF"
                     /locus_tag="KW011_000383"
     CDS             complement(53823..54722)
                     /gene="hemF"
                     /locus_tag="KW011_000383"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HBH7658822.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(54726..55595)
                     /gene="amiA"
                     /locus_tag="KW011_000384"
     CDS             complement(54726..55595)
                     /gene="amiA"
                     /locus_tag="KW011_000384"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HBH7658823.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLPGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            55809..56234
                     /locus_tag="KW011_000385"
     CDS             55809..56234
                     /locus_tag="KW011_000385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HBH7658824.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            56221..56670
                     /locus_tag="KW011_000386"
     CDS             56221..56670
                     /locus_tag="KW011_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HBH7658825.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            56731..57306
                     /locus_tag="KW011_000387"
     CDS             56731..57306
                     /locus_tag="KW011_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HBH7658826.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            57402..58301
                     /gene="yfeX"
                     /locus_tag="KW011_000388"
     CDS             57402..58301
                     /gene="yfeX"
                     /locus_tag="KW011_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HBH7658827.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(58359..59663)
                     /gene="pbp4b"
                     /locus_tag="KW011_000389"
     CDS             complement(58359..59663)
                     /gene="pbp4b"
                     /locus_tag="KW011_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HBH7658828.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(59668..59841)
                     /locus_tag="KW011_000390"
     CDS             complement(59668..59841)
                     /locus_tag="KW011_000390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylglucosamine transporter subunit
                     IIBC"
                     /protein_id="HBH7658829.1"
                     /translation="MGLNSAFGPSGLVALPLMTSAQGILPAMAVYAGGILVAWVCGFI
                     FTTLFGCRNVNLD"
     gene            complement(59854..61092)
                     /gene="murP"
                     /locus_tag="KW011_000391"
     CDS             complement(59854..61092)
                     /gene="murP"
                     /locus_tag="KW011_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HBH7658830.1"
                     /translation="MAKEISSELLNTILTRVGGLGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMSDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIA"
     gene            complement(61096..61992)
                     /gene="murQ"
                     /locus_tag="KW011_000392"
     CDS             complement(61096..61992)
                     /gene="murQ"
                     /locus_tag="KW011_000392"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBH7658831.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            62156..63013
                     /gene="murR"
                     /locus_tag="KW011_000393"
     CDS             62156..63013
                     /gene="murR"
                     /locus_tag="KW011_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HBH7658832.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            63142..63933
                     /gene="ucpA"
                     /locus_tag="KW011_000394"
     CDS             63142..63933
                     /gene="ucpA"
                     /locus_tag="KW011_000394"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HBH7658833.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            64104..65120
                     /gene="cysP"
                     /locus_tag="KW011_000395"
     CDS             64104..65120
                     /gene="cysP"
                     /locus_tag="KW011_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HBH7658834.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            65120..65953
                     /gene="cysT"
                     /locus_tag="KW011_000396"
     CDS             65120..65953
                     /gene="cysT"
                     /locus_tag="KW011_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HBH7658835.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            65953..66828
                     /gene="cysW"
                     /locus_tag="KW011_000397"
     CDS             65953..66828
                     /gene="cysW"
                     /locus_tag="KW011_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HBH7658836.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            66818..67915
                     /gene="cysA"
                     /locus_tag="KW011_000398"
     CDS             66818..67915
                     /gene="cysA"
                     /locus_tag="KW011_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HBH7658837.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            68049..68960
                     /gene="cysM"
                     /locus_tag="KW011_000399"
     CDS             68049..68960
                     /gene="cysM"
                     /locus_tag="KW011_000399"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HBH7658838.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(68963..69331)
                     /locus_tag="KW011_000400"
     CDS             complement(68963..69331)
                     /locus_tag="KW011_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HBH7658839.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            69436..70287
                     /gene="pdxK"
                     /locus_tag="KW011_000401"
                     /gene_synonym="thiJ"
     CDS             69436..70287
                     /gene="pdxK"
                     /locus_tag="KW011_000401"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HBH7658840.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT        16701 a        18358 c        19437 g        15807 t
ORIGIN      
        1 aaatggcgaa tggcaatggc gaacggaatc acaaacaaat tggcgataca gtgctcaaac
       61 ccactggcga caaacagggt gatgggcaaa atcatggcga cgattttgtc gcataatgaa
      121 cgcccgcaat aactcatcca cagcgccaga caaaccatca gattgcacat aatgccgagg
      181 ctgacagatt cagtaaatgt atgatgcatt ttgccctcgg cgcagtgtaa aaccgcgact
      241 ccccacatcg cgttttcact catcaccagc ccggaaaacc agattaacaa actgaaacag
      301 gcaatacctg ccagattacc gcaggccacc agaagtgcgt taatcagcca agttcgccaa
      361 ctaataacgc cccgactttt tgccatcacc gtcattaccg acgaggtgaa caggctggtg
      421 ccacaaaccg ccagcaaaat aaagccgagt gtaaagcaaa ggccgcccac cagatgggtt
      481 aatgcctgtg aagacggggc atcggcgata acggaaaggt aaaaagtaaa tccaatcgcc
      541 ataaacacgc cagcggacat cgccattaaa aagaccgaca ctttgctgcg ggcaatttta
      601 tgggcagtaa tccgttcagc gattgccgct tttctggcgc tcaactgcaa gtcgaaagag
      661 agtttgtttc tcatgcatac tcctgcaaaa gcagattaca acacctcgcg aaccgagagc
      721 cccagacgct gcattcgtga cagcagcgtg gtgcgcttca tccctaatcg tgtcgccgcg
      781 ccacggggcc cggcaacaat gccattggtt tctcgcaata cctgaacaat gcgctgacgt
      841 tcttcttcgt cattctctgg cgtcgtcggg tgcagcaact gcgccattga actgcgaagc
      901 gctgaatctt catttagcgt cggcagtaaa tggctttgtc ggacattaag atgtaaattc
      961 agactgttac cgcgagtcag cagtaccgct cgctcaatca cgttttccag ctcgcgcacg
     1021 ttgcccggcc agtcccacga catcaactgg cgtagcgcct cggtcgggat ggcgtcaatt
     1081 gaacgattca tatggcgcgc cattttttgc gtgaaatgct ttgctaaaag agggatatct
     1141 tccggacggt cgcgtagcgg cggcaattcc agtgggaaga cattcaggcg ataaaagaga
     1201 tcgctgcgaa actggcgatc ttcaaccatt tgccacaaat cacggttggt ggcggcaatg
     1261 actctgacat tcaccgggat cgttctactc ccgccgagac gctcaatctc ccgctcctgc
     1321 aatacgcgca gcagtttagg ctgaagttct aacggcagat cgccaatttc atcaagaaac
     1381 aacgtgccgc catcggcaat ttcaaaacga ccacgatggg tattaatcgc accagtaaac
     1441 gcccctttgt catgaccgaa taactcactt tccagaagac tggcggggat ggcagcgcag
     1501 ttgattttga ccagcggctt gtcgcgtcgc gggctaagtt gatggatcgc tctggcgatc
     1561 acctctttgc cggttccggt ttcaccacaa atcaacactg tactgtcgct cttcgccaca
     1621 atatctacct gctggagcag gtcttccatt gcctggcttt gatagataat gtcaccgacg
     1681 cccagattcg aaaggagctg ctcgctaagc tggtggtttt cttgctgcaa actttcctgc
     1741 aaatcggtca tgctacgcca ggcatcggca ttgccaacgg caatagcgat gcgatcggct
     1801 atgtgttgta gtagctgaca gttttcctca ctaaagagag tggaagaggt atgcgccagc
     1861 aacaatgcac ccggtgtatg gttgccaaag gtaagcggta taaggagcgc agattcgcag
     1921 ccgttagatg caagtaagag taataacggg tcgcgttgcc agagaaacag atcgtctgca
     1981 cggtgcgtca aggtcggttg tcgggtttgt agcgtttgtg tcagcaatac actgtcgcca
     2041 ggcatattgc gtggcagaca cgcacaatgt gaggcagaaa gatcgctgca ccacaggcta
     2101 aacttctcgt tctttcgatg atcgcccagt accatactga ctgaagccag accgaaaaaa
     2161 tgatggatct cacgagcgac gtcagcgatc agatcgtcga gatcaagatg tgacagcact
     2221 gcattagtaa tatcaaccag tacccgatga tgatcgcgat ctttctccag caaacgcagc
     2281 gccacctgct gttcttcatc attgagccat gattgcagaa ccggggtgat tatccgcgcc
     2341 agccagttaa acagtgtgca atcctgggga gtaaaacggc agccgtgggt gcgaataaag
     2401 cgtagctccg ccattccact actgccgaga aggacggcgt gacgcgaata atcgaccatc
     2461 tcgccctgat actctgttgt tgccgtcaca tcgctgccat catcatggta gcggtaatag
     2521 aagttgcttt tgagaacatg cagttcaata agttgtaccg ggctgaacgg cggcggcagc
     2581 gggatgaagg cgcgtaataa agacgcctta ccgtgtttct gcgctaaccg ctccgcgagc
     2641 attccgttta ccgcttcaat tgttattcct tgcggcgggg caaacatcgc ctcgtctgac
     2701 atagccatca acagtcctta ttaatgagaa atccttctca cgcaattttt ctggccatca
     2761 gatagatggc cggttcctgc acaatttcat ccaacagatg aatgaattga atgctacgcg
     2821 cctgccacac agacgtcgcg tcatccgcca gtggcgcgaa tgcccgggtc aacagggcgg
     2881 tgtggctggc gtcaataaca atctcaccaa aagtcatcac ccccagcatc ttacgtcggg
     2941 cttgctcctc ttcgaccagt gcaagccaat cttcgtattc cgtcagtggg caggggaagg
     3001 cgacattcag acaatcaatc acgccaacgt gatgaccgat agccagcgag taatacatta
     3061 cctgagagct gtgttccggc atctcgtcgc tactggcgac aaactttttt cgcagcgtcc
     3121 agaaaacaat ttccccgcac tcttcagtca tgtaaccccc ggagtacgcg aaagagttgc
     3181 tgaacgatac tattgagacg tggatcgtcg gcatcgcgca accaggtgtt aacccgattt
     3241 cctgtaggat cgtcgcttaa atggcgcagg agccgatcgc aaatttctcg cccctgacga
     3301 tagcccgcca gccgccgcgc ttctcgctca atggcgatac gctgtgatgg cgggatctgc
     3361 ggccacaacg gttgcatagt caccccggtg ggatcgcgat aatccaccgc gtgaatcttc
     3421 tgttgcagca aaccgagcgc cacggcgaaa ccgtgaatgg tggcggccgg tgttggcggg
     3481 cagccgggga tccaaacatc aatggggaca atggtgtcgc tgccgcccca gacgctgtag
     3541 agatcgtgga aaataccgcc gccgacaccg cacgcgccgt aggaaacaca aattttatga
     3601 tcgggggcag actcatacgc ccgaagtgca ggcatacgca tcgcccgggt gactgcgcct
     3661 gtaaataaca aaatatcggc gtgacgcggt gatgaaataa ccttaatgcc aaaacgttct
     3721 gcgtcgaata ctggtgtgat ggcggcaaaa atttcgattt cacaggcgtt gcagccgccg
     3781 caatcgacgc gataaacgta agccgagcga cggatatcct gtagcaaatg ctgcttcatc
     3841 ttttgcgttt gctcgtccag cgtgatgggc tggctgacat gttgtgtaag cactggactc
     3901 atagctgctc cttagccacc agcggtacat ctgtatcgtc gttgagcagc gtcgcgcgtt
     3961 gtttgcactc cgggcagacg ctcgcttgcg cccataacat ttcgcggttt tgtggcgcat
     4021 tttgttgctg tgccaacaat tcagcagcca gtgcgacggt tttttgcggg gcaaacgggc
     4081 gttcgcaacg gctgcaacgt tgtagatgga acgtcgcgcg ggtatagaga tcggctttat
     4141 tggtgacggt cagttcaaag ttattggtaa gctggatggc tctggtcggg cacacttctt
     4201 cacaacgtcc gcagtaaata caacgcctca gatagagctg ccaggtgcgc gaattttgct
     4261 ggtcgtcggt ctggatagtc agcgcatttg ccggacaagc acaggcgccg caggcaatac
     4321 attggctggg catcaggtcc ggtttcccgc gaaagcctgg gctgacctcc agtggcgcga
     4381 agggatattt caccgtcgcg gttccggcgc gcataatagt tttcagtaac ttcagcatct
     4441 tctgtcctta cttcagcggc gaacggttac gatcaatgcc gtagcgttcg atctctttat
     4501 acggcacggt ttttgactgg cgcttgcgca catctaccag cgtcacacgg tcggtacagg
     4561 agtagcaggg atcaaggcta ccgataatca gcggtgcgtc agaaacggta ttgccgcgca
     4621 gcatgtaacg caacaccggc cagttggcgt aggtggcggc acggcagcgc cagcggaaca
     4681 atttttggtt atcaccgagc atgctccagt gcacgtcttc accgcgtggc gcttcaacaa
     4741 agcccagcgc gaatgcgtga ggtttatagc taaagccttc ggtcagcagt ggggtatccg
     4801 gcatgttgtc gagggcaaat tccagcattg ccagcgaatc aaacgtctct ttgacacgga
     4861 ccatcacgcg ggagaaaaca tcgccgccgg taaaggtaaa cagtgttttt ggaatattgc
     4921 cgtagtcggc gtaggggtga tcaaagcgca aatcacgggc aaaaccactg ccgcggatca
     4981 gcggccctac agggctataa tcacgggcga tttgtcggtc gagaatgcca atgccctgag
     5041 tgcgttgttc catattcggc gtggcaagca gcatctctac cagctccgac acgtcggcgc
     5101 gcatctcgcg caccagtttc agcgtttgca gacgttgctc tttgagaata tcgcggcgaa
     5161 caccaccaat cagattcaga ccgtaggttt tacgcgaccc ggtcagcaat tccgccattg
     5221 tcatcgactt ttcccgcacg cggaaaaatt gcataaagcc ggtatcaaat ccaacaaaat
     5281 ggcaggagag gccaaggttg agcaaatgac tgtgcagacg ttcgacttcc agcagaatcg
     5341 agcgaatagt atgcgctcgt tgcggcacct caatccccag tgcattttca accgagttgg
     5401 tataggccac actgtgggca aaaccgcaaa tcccacacac gcgatccgat aagaaggtca
     5461 cttcgttgta gcccatccgc gtttctgcca gtttctccat gccgcgatgg acataaaaca
     5521 ggcggtaatc agcatcgaca atttgctcgc catccacaaa caagcggaag tgacccggtt
     5581 catcggaggt gatatgcagc gggccaatag ggatcacccg cgcatcgcta ttgccctcat
     5641 tgataaacgg atacgtttcg gtatcagtcg tcggttcagg gcgcagtcga taatccatcg
     5701 catctttgcg cagcggatgc atatcttccg gccagtcatc gggcaacacc aggcgacgct
     5761 gatccggcag gccaaccgga atcagcccgt acatatcgcg aatttctcgc tcgccccaga
     5821 ccgcggcagg gacgcgcggt gtgactgacg gaaactcccg actgtcggca tcgaccagtg
     5881 ccttcaccac gatccagcat ttttcggccc cttccattga aagggcataa taaaccgcgt
     5941 aatgaccgtt aagtgtccgc tcgtcgttgc caaacaggac tggaagccag ccatcatgtt
     6001 gataataaag ataatgtaca acgtcaggca gcagattgat tttcaccgta atggtgacct
     6061 gttcaggcgt ttgtcgctct tcatccagca ccgcgccggg gaactgcgtt ttcagggcgg
     6121 cgagaatcgc ttcgccacga tttgacgatg aattaacgtt cactgtgttt ctccgaaaca
     6181 gatgcggtgc ctgaaggcaa aaaatcatgc caggtgctac gttgtgcagg caggtcgtgc
     6241 gtccctgaga ggactatagt ggaagcgccc gccaggatcc tgatgacagg ttgtggaata
     6301 tgcgttccca tcgccagcat catgaccagc agaatcacca ttggcgaggt ggtcaaccag
     6361 ccgagatcac cccggttaac ggcctgcggc ggtttcgcca ttaacacccg cgctgccatc
     6421 cgtaccaggc ccgccagcac cagcgttaac agcaataaca gcaggacgat aatcagcagg
     6481 tgattacgtg ccagtccggc ggtaatggtc ataaattcgc taagaaaaat gttgaagggc
     6541 ggcatccctg ccagcgccag cgcaccgccg ccaaacagca cggcggtaaa tggcatgatt
     6601 ttgagcatcc cacagacgac gttgagatcg cgcgtgccgt acttgagcag tacattgccg
     6661 gaaccgcaga acagcagcgt ttttgccaga ctgtggttta agatgtgcag cagcgcggca
     6721 aaaattccca gcggcccgcc aatgcctagc gccaccgcga ccagccccat gttctccacg
     6781 ctggagtacg ccagcagacg cttaatgtcc cgctgtacca gaatgaaaaa tgccgccacg
     6841 gcaaccgaca acatgccgaa gatgagcaac aaccggttgg ggaaatcgct gccgatggct
     6901 tggcaaataa tgatgtaata gcgaatcagc accaacagcg cgcagttcag caatacggcg
     6961 gagagcaggg cgctgaccgg actcggcgct tcactgtgag catccggcag ccaggcgtgc
     7021 atgggaaata gcccggtttt ggtaccaaag ccaattagca caaacacaaa ggccaacagc
     7081 attaatgttg ggtcaagcaa ggacgattgc ttaagaacct cgctccagaa tatcgccatt
     7141 tctgcctgcg gcataacgct ggcggcgttg gcgtatacca gcacggtacc gaacagacca
     7201 aaagcgacac caacagtaca aataatgatg tacttccatg ctgattccag cgatgaacgc
     7261 tgaccgtaaa tgcctaccag aaacgccgag cttaaggtgg tggcttcgat cgccgcccac
     7321 atcacaatca ggttattgct ggtaacaacc agcagcatgg tgaacaaaaa cagatggaag
     7381 aaaccgtagt aatcgcacag cgtaacgggt gaaagctcgc cgtgtgccac ttcatgacgc
     7441 atgtagccaa tcgagtaaac accggtgaga aagccaatca caccaagaat ggcgaggaac
     7501 aaaccgccca gaccatcaat atgaagccac agtcccgcag cgaatatttc tcctgcatca
     7561 gcggcagttt ggaccaccca gagtgccaga atcagcagca gtgtgatccc taagctatgt
     7621 aatacagtca ccgtacaagt cgcagagagc ccccgtttcc ggcaggcaaa acagagcagc
     7681 gaaaaaagca gcggcgtgag caggagtaaa gcgaacatca cagaataact catctcatta
     7741 tcccttcagc gcggtcaagt tgttcacgtc cagcgtgccg tgggtacgcc atatttttct
     7801 tgccagtaac accatcacaa tgacggcgaa gatggcgtcg gtagcgatac ctatttccac
     7861 cagttccggt gctcgccagg caagaagcgc cagcaccaga tgggagccgt tttccatcag
     7921 gcagtaacca aaaatttgcc gcaggatatt gcgctggctg acaatgcaca gcaggccaag
     7981 cagaaaatga cctaacgcta ccgccagcgc cggtttcagc ccggtagcca tcggtagctt
     8041 cacgggctga acgacaaatg cgcaaagcag gacaattaac gccgcgagca gtgccatcat
     8101 tgccggaccg aataacgctt tttccgggat gttctgggga atatttcgtg cagcgtaagt
     8161 catgattaac ggtaccagca gcactttggt gataaaggcg ctggcggacc agatcagcag
     8221 ttgctctgcg gcgaacaggc acgagagagt ggcgaaaata gacaccagca ccagcgactg
     8281 gcaggcgtaa aatccgcatg acaggcgata gcttttgacg ctaatcacaa acagcgatgt
     8341 cagcatcatc agtcccgcca gattatttac gatcatagaa ccggtcattc atcatctcct
     8401 tactgcaacc aggtcgcggc gattacgctg gaaatcagcg acatcacaat cagtacaatc
     8461 aacaccaggc gcattgaccc tggcagcggt gcggcatcgg cgacggcctc gctcggtttg
     8521 cccggcacga cgcgaccaaa ccagcgaata aaccaggcga aactggcgac cgattcaatc
     8581 atcagcagaa tcatggcggg cagcaggatc cagtactcca ctgacaacgc aaaaccgacg
     8641 gcaaacagcg ggaatttact gaagaagccg ttgaacggcg gcacaccggt aatcgccagc
     8701 gctgccacgc agaaacccac gcttggcaac ggcagggtgt gcaatacgcc acgcagacgc
     8761 ggcaacaagc gcgtgccgca gctgtaactc agcgcacctg ctacaaggaa aaacaggctt
     8821 ttagcgaacg cgtggttgac gatgtaggcg atgctaccct ccagcgccag ccgcgagccg
     8881 aagatggaga gcgacaagcc gaagaacatc cagccaagtt gagtgatggt cgaccaggcc
     8941 agcaaccgct tcatatcctg ctgtggcaaa tacatcagaa agccataaag aatggtgacc
     9001 agcgccatga ccatgccaac gccgccaatc acatgcggga tattgccgcc gtcgataatg
     9061 gcgcgggcaa aaatgtaaac gcccactttc accatcgatg cggcgtggag ataggcactg
     9121 atcggtgttg gcgcttccat tgcatccggt agccacgctt gcatcggtag ctgggccgat
     9181 ttcccccacg cggcaaacag gatgccgcca taaaccagat aacgtgcgtc gccgtgtaac
     9241 tcgctcatcg cgctaagcgc aaacgttccg gtctgcaaaa acagcgtggc ggcggcaaga
     9301 tacaacccca acgagccgat atgagtgata agtaacgctt ttagtgctga aagctgtgct
     9361 ttatcgctct ggtaataact gatcaacgcc caggagcagc cgcccgtaat ttcaaaaaac
     9421 aacaactgac cgagcagcgt cgaggagagt accagtcccg ccatcgcgcc gataaacacc
     9481 agcaaaaatg cgtaataacg attcgtgccg ttatgcgggt gttcgcgatt tttatccgtc
     9541 agataacccg tggagtagat cgtgaccagc aaaccgagaa acaccaccac aaacagaatc
     9601 agcgtactca tgcgatcaat gaccaagcca aacaatgcca catcccccac gttgaccaac
     9661 gtcaacgtga cggcaacttt atcggcctga taaaacgcgg agatcagcga caacatgcac
     9721 agcgtggtca gtgcggcgaa caaaaccccc attcggcggc ccgacgttgt ggcgaaaacg
     9781 aaacgaccag tgcgccgata aaaggcagca ttaacgtcgt cagagcaaga ttttccatat
     9841 tccgtcagtg ctccttacag accggttaac cagaagaccc atgcaagcgc agcaaggctg
     9901 aagccaagcc aggtcacatg gtgaatgagt aaaaaacgcc cgcgtgccag cgtgttttct
     9961 gcgattgagg ccagtacaaa aatcagcaaa accttgagca gcgtaacgac aagtgaagtc
    10021 agcaggcagg cgagagaaag ttcttgcgcg cgcccaaagg gcagaaacag ggccacaaac
    10081 agtgctgcca tcacgacctg tttcagcccc agcccccatt tcgctagcgc cagcccggca
    10141 ccggaatatt cggtcagcgg gccttcctgt aattcctgtt ctgcttcagc aacatcaaag
    10201 ggaatttttc ccatctcaat gaagcaggca aaaccacagg ccagtaacgc cagtacggtg
    10261 gttagcggcg agttccagcc cgttgccagc gtattgctga tcatctcgat atgcgtggaa
    10321 cctgctatca gcgccaatac cagcagtgag agaatgagca tgggttcgac cagaatgccg
    10381 agcgtcaact cgcgactggc accgactccc gcaaacgggc ttccggtatc cagccctgaa
    10441 agagcaaaga aaaaacgaaa cagggcaaga agatagataa gggtgatcag atcgccgccg
    10501 cccgcaaaag gggaaacggt aataaatagt ggtagggcca tcgccagcac cagcatgctg
    10561 ctgattaata cccacggcat caggcggaac atcagacctg aagatgtcgg cgcatcttcc
    10621 tggcgtttaa acagtttgtg gatatcgcga taatcctgcc agatccccgg cccgcggcgg
    10681 gagtgcatac gcgcgcgtat ctgccgggaa atacccgtaa aaagtggggt tagcatcagc
    10741 agaatcacgg cctgtgctaa cgcaaaaatg accagatatc cgtcgcaaag agtttgtctc
    10801 atggtgattt ccttagacgg caatagcgat tagcagcaca accagtgcgg cgaccacata
    10861 caggcaatag agacgaaagt cgccgctttg cagatgctgt atttctttgg ccagccgttg
    10921 ggtggcgctc acgatggggc ggatcacccg ctcatcccag aagggttctg tgctctgagc
    10981 cctggcggtg acgtgcgcaa gacctttatt tagcctcagc gtagggtcga gctgttgtcg
    11041 tagacgaaat agcgcagaaa agaccacacg cagcggctgc atcacgccat tgcctgacgg
    11101 ggccatcgca ttttcccagc cgtagccgca tgcccaggca tctcctgtgc gacgaaacgc
    11161 tgcgcggcgc gaacgacacc acagccagta aaggccaggc agcaaaggca tcgccagtag
    11221 taacagcaac agtaatgaag gagtgacccg tgtatgaaac gtgccgggta caagtgctat
    11281 tccgctggcg acagtgacgg gaggggtatt ggtaaacgca tgggcaatgt gcattatctt
    11341 cggtgccagc caactggcac taagcgcaat taatacgcag agtgccgcga gcagtagcat
    11401 ggcgacgatc atcgtatttg gcacttcctg tgcctcttca gcgtgtgtgc tgcgcggcgc
    11461 accacagaaa gtaataccgt acattttgac gaagcacact actgccagcc caccagtgac
    11521 tgccagcatt accatagcaa taggacccgc aagttgtagc gctacggctt ccacacgact
    11581 tagtgagaat agcgactgcc aggtgtacca ttcgctgata aaaccattca gcggaggaat
    11641 ggctgatatc gccaggcaac caatcaggca tgctgcggct gtccacggca tccgtttcgc
    11701 cagtgccccc attttttcca tgtcgtgggt atgcaaacgc gaaataatcg cacccgcgcc
    11761 gagaaatagc agccctttga aaagcgcatg gttgagcaga tgaaacagtg cgccgagcag
    11821 tccaaccacg gtgagcagcg ggtcgtgcag tgacagaccg accatcgcca caccgaccgc
    11881 cagcaaaata atgccgacgt tttcgacggt actccaggcc agcagccgtt tgatatcctg
    11941 ttccgccagt gcatacagca cgcccaggag cgcggagatt gcgccgatcg ccatcaccag
    12001 aatgccccac cacagcggca aacccgtttg cgccagcaga tccatcgcca ctttcaggat
    12061 gccgaaaata ccgattttga ccattacgcc agacatcaac gctgaagcgt gcgatggtgc
    12121 ggcagggtga gcgcgcggca accagctgtg caacggcatc atcccggctt tcgcgccaaa
    12181 accgaaaaag gccagcagga acaccgccga cgccagcccc ggagaaagtg aaagcgtgcg
    12241 gaaactggca aaatcgaggc tgccgctttc gcgccccatc agcaagaagg cgatcattat
    12301 cagcaccgat ccggcgtggg cgataaaaaa gtagagcatg ccagcgttga tcgacgtttt
    12361 atcctgcctg gcaatgacca gaaaccagga agacagcgac atcatttcaa acagcacgat
    12421 gaaccaaaaa gcgttgtcca tcaccagcag ggcaaccatc gatgcgatga aaatattcat
    12481 aaagaagccc atcgccgccg cgcctttgcc ctcgtattcg cgcatataag tcaatgaata
    12541 gagagaacaa accaccacca gcaaggagat aaccagcacc ataaatgccg agaggctatc
    12601 cattcgcagg gttaaatcgg caaattcaaa aggcgtggca aaacgggcga ctaaagttac
    12661 gcctgcatgt aattgcgtaa ttccgctgat gatgccaatc acgccgccta ccagcgatgt
    12721 gatgccggaa agcttaatag ccagtctgtc cagaccgagt aaaaacagcg aagccagact
    12781 ggcaaacaga tagaggatca gcgaccaggt taataattgc agggcatcca ttagcgttgc
    12841 tcctgagtga gggaaagggg atcctccccg ccattggcaa accagcttgc ggcaaggcgc
    12901 tgtttttctt tcatctggcg ttgcaggcta tcgtcggtga tcagccgtaa cgcctgattc
    12961 gggcaagcgc gaacgcaggc cggaccttct ggcaagaaat cacacaggtc gcatttcacc
    13021 gcgacggtct ggacacccgg ttcccagcgc agcaacgcat gttggggcgg cacgttttct
    13081 tcgccgtctt tgagtgagcc ctcagcctga aaaacatatt gcgcaggggc attcaccgga
    13141 cggcttcctg aagcgctgat tgcgccaaat gggcagacca cggcgcaaag cttgcagcca
    13201 atacagaggc tttcgttgag ttggatcgca tcatccctct gagagatggc attgaccggg
    13261 cagacctgca ggcaaggggc ttcctcacag tgatgacaca cgacaggggc agtgagtgtt
    13321 gacgtcttcg ccagggccag gcgcgggtgt tgctgtaaac cttgcgtttt atgcacgtcc
    13381 gaacaggcag cgagacaggt attacatcct gtacaccaca gtggttcggc caccacaaag
    13441 cggttcatgt cggctccttg aaaatgatat gaaacattgc atttcatatt gcataaagga
    13501 tgccattctg attaactcaa tatattcaat aagttatgta ttaactggat tctgaacggt
    13561 acaaaaatga tggtcattaa cgttcagcaa aaaatggatt cgacacccgt gtcgcagggg
    13621 aaagcgagaa tgtgatcttg atcacgagaa gtgaggtatg ctggccgcaa gcgcagccag
    13681 cacggaatgg agcaaagtaa tcaggcgtgt tctttcagcc agttcagcac aacgtcgtgg
    13741 tgattgctgg ttttgaaatc gtcaaagaca tgttcgattt tgccatcagc gtcaatcagg
    13801 aagctgatgc gatgaatgcc atcgtaggtt ttgcccatga aggacttttc accccagacg
    13861 ccgaattgtt cgcacacctg gtggtcctca tcagacagga gcgtaaagtt aagcagctct
    13921 ttttccgcaa aacgggagag tttttcgggt ttatcggtgc tgatacccag cacatcaacg
    13981 cccgcttttt tcaactcatc catgttatcg cgtaagccgc aggcctgtac ggtacagccg
    14041 ggggtcatgg ctttcgggta gaaataaacc agaacacgct gtccctggaa gtcggtcaaa
    14101 ttaacttgtt ctccgtcttg atccggcaag ctaaatttcg gtgcgatatc accggctttc
    14161 agtggattca ttacttaact ccatcctgtt catcatgttg ggaataattg acgacgttaa
    14221 tactgccttg tgcattgagt tctgtacata gggctttgaa cgcttgctca atatttgctg
    14281 cgtccgcaga tgcggggctg tgggcggtta tctgaatatg caactgcgca gcccgttcat
    14341 tttcagcagg ttgcgtgcgc gacaccagct ccgcaatgtt catatgatgc gcgtcgaaaa
    14401 gtgctgtgaa gcgttcaatt aaatgcgggg agtctgccac atcgacctga acccagacag
    14461 atgctggcat tggcggacgc ggacgcgccg tcgtgcgctt catcacgatt aaaagatcca
    14521 gctcggcacc tttcaacggt aacgttgatt caatcagagt aatggcattc catgaaccgg
    14581 aaagcagcat gataaacgtg aactcttctc ccagcatcgc caggcgactg tcttcaatat
    14641 tacagccgca actactgaca tgacgggtga tggtgttcac aattccaggg cgatcggcac
    14701 ccaatgcagt gatcaccaga taatgttgcg atgacagtgt caaactggtt attcctttaa
    14761 ggggtgagtt gttcttaagg aaagcataaa aaaaacatgc atacaacaat cagaacggtt
    14821 ctgtctgctt gcttttaatg ccataccaaa cgtaccattg agacacttgt ttgcacagag
    14881 gatggcccat gttcacggga agtattgtcg cgattgttac tccgatggat gaaaaaggta
    14941 atgtctgtcg ggctagcttg aaaaaactga ttgattatca tgtcgccagc ggtacttcgg
    15001 cgatcgtttc tgttggcacc actggcgagt ccgctacctt aaatcatgac gaacatgctg
    15061 atgtggtgat gatgacgctg gagctggctg atgggcgcat tccggtaatt gccgggaccg
    15121 gcgctaacgc tactgcggaa gccattagcc tgacgcagcg cttcaatgac agtggtatcg
    15181 tcggctgcct gacggtaacc ccttactaca atcgtccgtc gcaagaaggt ttgtatcagc
    15241 atttcaaagc catcgctgag catactgacc tgccgcaaat tctgtataat gtgccgtccc
    15301 gtactggctg cgatctgctc ccggaaacgg tgggccgtct ggcgaaagta aaaaatatta
    15361 tcggaatcaa agaggcaaca gggaacttaa cgcgtgtaaa ccagatcaaa gagctggttt
    15421 cagatgattt tgttctgctg agcggcgatg atgcgagcgc gctggacttc atgcaattag
    15481 gcggtcatgg ggttatttcc gttacggcta acgtcgcagc gcgtgatatg gcccagatgt
    15541 gcaaactggc agcagaaggg cattttgccg aggcacgcgt tattaatcag cgtctgatgc
    15601 cattacacaa caaactattt gtcgaaccca atccaatccc ggtgaaatgg gcatgtaagg
    15661 aactgggtct tgtggcgacc gatacgctgc gcctgccaat gacaccaatc accgacagtg
    15721 gtcgtgagac ggtcagagcg gcgcttaagc atgccggttt gctgtaaagt ttagggagat
    15781 ttgatggctt actctgttca aaagtcgcgc ctggcaaagg ttgcgggtgt ttcgcttgtt
    15841 ttattactcg ctgcctgtag ttctgactca cgctataagc gtcaggtcag tggtgatgaa
    15901 gcctacctgg aagcggcacc gcttgcggag cttcatgccc cggctggaat gattttgccg
    15961 gtgacctccg gtgattatgc aatcccggtg accaacggta gtggtgctgt cggtaaggcg
    16021 ctggacattc gtccaccagc ccagccgctg gcactggttt ctggcgcgcg tacccagttc
    16081 acgggcgata ccgcttcatt gctggtggaa aatggtcgtg gcaatactct gtggccgcag
    16141 gtggttagcg tgctgcaggc gaaaaactac accatcaccc aacgtgatga tgctggtcag
    16201 acactgacca ccgattgggt acaatggaac cgtctggacg aagacgagca gtatcgtggt
    16261 cgttatcaaa tctctgttaa gccgcagggt tatcagcagg cggttacggt taaactgttg
    16321 aacctggaac aggcgggcaa accggttgca gacgcggctt ccatgcagcg ttacagcacg
    16381 gagatgatga acgttatttc cgccggtctg gataaatctg ccactgacgc cgcgaacgct
    16441 gcgcaaaatc gtgcctccac cactatggac gtacaaagtg cagctgatga caccggttta
    16501 ccaatgctgg tcgtacgcgg gccgttcaat gtggtttggc aacgtctgcc agcggcgctg
    16561 gaaaaagtgg gcatgaaagt gaccgacagc acccgttcgc agggcaacat ggccgtaact
    16621 tataagccgc tgtctgacag cgactggcag gaactgggcg cgagcgatcc aggcctggca
    16681 tccggtgact ataaactgca ggttggcgat ttagataacc gcagcagcct acagttcatc
    16741 gatccgaaag gtcatactct gactcagagt cagaacgacg cgctggtagc tgtcttccag
    16801 gctgcgttta gcaagtaaaa atacagggct ggaatcatcc ggcccttttt tctgatatga
    16861 tacgcaaacg tgtgcgtcgg caggaaaacg cgattttagc ggtaattcgc acgaaatttg
    16921 tttgtcggac gtagttcgga taaggcgttc acgccgcatc cgacaaaaca tccggcacac
    16981 cagacagcaa aagattttaa aacgttaatt cacacccagg agtgataaag atgcaaaagc
    17041 aagctgagtt gtatcgtggt aaagcgaaaa ccgtatacag cacggaaaac ccggacctgt
    17101 tggtgctcga attccgcaat gatacgtcag caggggatgg cgcgcgcatt gagcagtttg
    17161 atcgcaaagg tatgttgaac aacaagttca actacttcat tatgagcaaa ctggctgaag
    17221 cgggtatccc gactcaaatg gagcgtctgc tctccgatac cgaatgtctg gtgaaaaagc
    17281 tggatatggt gccggttgag tgtgtcgtgc gtaaccgtgc tgcgggctct ctggtgaaac
    17341 gtcttggaat cgaagaaggt attgagctga acccgccgct gttcgatctg ttcctgaaaa
    17401 acgatgccat gcacgatccg atggtcaacg aatcttactg cgaaaccttt ggctgggtga
    17461 gcaaagagaa cctggcgcgt atgaaagagc tgacctacaa agcgaacgac gtgctgaaaa
    17521 aactgttcga tgatgctggt ctgattctgg tcgacttcaa gctggagttt ggtctgtaca
    17581 aaggtgaagt ggtactgggc gatgagttct ccccggacgg cagccgcctg tgggacaaag
    17641 aaacactgga gaaaatggac aaagaccgtt tccgccagag cctcggtggc ctgatcgaag
    17701 cctatgaagc cgtcgcccgc cgcctgggtg tacagctgga ctgatttttc tgttcatcat
    17761 cttgccgtgc agctggcacg gcaagacaac cgctctcgga atatatgcat tgaacgctca
    17821 ttcctccgcc atttcatccc gctttcctgt ggtaatcttc ctctgaacca cctacgatca
    17881 tttctataat gaatatattg ttgaggtatc tatgcgttgg caagggcgac gtgaaagtga
    17941 caatgtcgaa gacaggcgta acagctccgg cggtccgtca atgggcggtc ccggttttcg
    18001 cctgccaagc ggtaaaggcg ggctgatttt actgatagtc gtgctggttg caggctacta
    18061 tggtgttgat ttaaccgggt tgatgaccgg acagccggtt tcccaacagc aatcaacacg
    18121 gtcaattagc ccaaatgaag acgaagccgc aaaattcacc tcggtgattc tggcaaccac
    18181 ggaagacacc tggggacagc aattcgagaa gatgggtaaa acctatcagc aaccgaagct
    18241 ggtcatgtat cgtggaatga cgcgtaccgg ctgcggggcg ggccagtcca ttatgggacc
    18301 gttctattgc ccggcggatg gcacggttta tatcgatctc tccttctatg atgacatgaa
    18361 agacaaactc ggcgcggatg gcgattttgc ccaggggtac gttatcgccc atgaagtcgg
    18421 tcatcatgtg cagaaattgt taggcatcga gccgaaagtt cgtcaactgc aacaaaacgc
    18481 gacgcaggcg gaagtaaacc gcttatctgt gcgtatggaa ctccaggccg actgttttgc
    18541 cggtgtctgg gggcataaca tgcagcagca aggcgatctg gaagaggcgc tgaacgcggc
    18601 gcaggccatc ggcgatgacc gtttacaaca gcaaagtcag gggcgagtag taccagacag
    18661 tttcactcat ggtacttctc agcaacgcta cagctggttt aaacgtggtt tcgacagcgg
    18721 cgatccggca caatgcaata cttttggtaa aagcatttaa cttccggggc aaggatggct
    18781 gaactgactg cgcttcacac attaacagcg caaatgaaac gtgaagggat ccgccgcttg
    18841 ctggtgttga gcggggaaga gggttggtgt tttgatcatg cgcttaagtt gcgtgatgcc
    18901 ttacctggcg actggctgtg gatttcgccg cagccagatg ctgaaaacca ctgttctccc
    18961 tcggcgctac aaactttact tgggcgcgag ttccggcatg cggtattcga cgcccgccac
    19021 ggctttgatg ccgctgcctt tgcggcactt agcggaacgt tgaaagcggg aagctggctg
    19081 gttttgttac tccctgtatg ggaagagtgg gaaaaccaac ctgatgccga ctcgctgcgc
    19141 tggagtgatt gccctgaccc tattgcgacg ccgcattttg tccagcatct caaacgcgta
    19201 cttacggcgg ataacgacgc tattctctgg cggcaaaacc agccgttctc gttggcgcat
    19261 tttacccccc gtactgactg gcaccccgcg accggcgcac cacaaccaga acaacagcaa
    19321 ctcttacagc agctactgac catgccgccg ggcgtggcag cggtaacggc tgcgcgtggg
    19381 cgcggtaagt ccgcgctggc agggcaactc atttctcgta ttgcgggcag tgcgattgtc
    19441 accgcgcccg caaaagcggc aacggatgta ctggcacaat ttgcgggcga gaagtttcgc
    19501 tttattgcgc cggatgcctt gttagccagc gatgagcaag ccgactggct ggtggtcgat
    19561 gaagccgcag ccatacctgc gccgttgttg catcaactgg tatcgcgttt tcctcgaacg
    19621 ttgttaacca ctacggtgca gggctacgaa ggtaccggac gtggtttttt gctgaaattt
    19681 tgcgctcgct ttccgcattt acaccgtttt gaactgcaac agccgatccg ctgggcacag
    19741 ggatgcccgc tggaaaaaat ggttagtgag gcactggttt ttaacgatga aaacttcacc
    19801 catacaccac aaggcaatat cgtcatttcc gcatttgaac aaacgttatg gcgaagcgat
    19861 ccagaaacgc cgttaaaggt ttatcagtta ttgtctggtg cgcactaccg gacctcgccg
    19921 ctggatttac gccggatgat ggatgcacca gggcaacatt ttttacaggc ggctggcgaa
    19981 aacgagattg ccggggcgtt gtggctggtg gatgagggcg gattatctca agaactcagt
    20041 caggcggtat gggcaggttt tcgtcgcccg cggggtaatc tggtggccca gtcgctggcg
    20101 gcgcacggca gcaatccact ggcggcgaca ttgcgcggac ggcggggcag ccggattgca
    20161 gtccatccgg cgcgtcagcg cgaaggcgtt gggcaacagc tcattgccag cgctttgcaa
    20221 tataggcctg gcctcgacta tctttcggtg agttttggtt acaccgggga gttatggcgt
    20281 ttctggcaac gctgcggttt tgtgctggtg cgaatgggta atcatcgtga agccagcagc
    20341 ggttgctata cggcgatggc actgttaccg atgagtgatg cgggtaaaca gctggctgaa
    20401 cgtgagcatt accgtttacg tcgcgatgcg caagctctcg cgcagtggaa tggcgaaacg
    20461 cttcctgttg atccactaaa caatgccgtc ctttctgacg acgactggct tgaactggcc
    20521 ggttttgctt tcgctcatcg tccgctatta acgtcgttag gttgcttatt gcgtctgcta
    20581 caaaccagtg aactggcatt accggcgctg cgtgggcgtt tacagaaaaa caccagtgac
    20641 gcgcagttat gtaccacact taaactttca ggccgcaaga tgttactggt tcgccagcgg
    20701 gaagaggccg cgcaggcgct gttcgcactt aatgaggttc gcactgagcg tctgcgcgat
    20761 cgcataacgc aatggcaatt ttttcactga ctccttcaat tttctcccat ggtcattgtg
    20821 agtcagcggc gtaaaattaa tgacatcagt taaggagttc gccatgaaac atgaccattt
    20881 tgttgttcaa agcccggata aacctgcgca acagttgtta ctgcttttcc acggtgtcgg
    20941 ggataacccg gtggcaatgg gggaaatagg cagctggttt gcgccgctat ttcctgatgc
    21001 gctggtggtg agtgtcggag gcgcggagcc gagcggtaat ccggcggggc gtcagtggtt
    21061 ttcggtgcag ggtattacgg aagataatcg ccaggcgcgt gtggatgcca tcatgccgac
    21121 gtttattgag atggtgcgct actggcagaa acagagcgga gtaggggcta acgccacagc
    21181 gctaatcggt ttctctcaag gcgcgattat ggcgctggag agcattaaag ccgaaccggg
    21241 ccttgcttca cgcgtcattg cttttaacgg acgctatgcg agcctgccgg aaactgcgtc
    21301 tacagccacg acaattcacc tgattcatgg cggtgaagat ccggttatcg atctggcgca
    21361 tgcggttgcg gcgcaggaag cgttaatcag tgccggtggt gacgtgacgc tggatatcgt
    21421 ggaggattta ggccatgcga tcgacaatcg cagtatgcaa tttgccctgg atcacttgcg
    21481 ttataccatt ccgaaacatt atttcgacga agcgctaagt ggcggtaagc ctggcgatga
    21541 tgatgtgatt gaaatgatgt aaaagacaga ttacctggcc tgataagacg cgtgagcgtc
    21601 gcatcaggca tcgttcacca ttgccggatg cggcgttaac gccttatccg gcctacggtt
    21661 gagcactcag cttacttctt cggttgatcc tttttcggcc agtcgtcgtc atcgtcccac
    21721 ttatcgttga aatcacgatg cgggggaagc tccggcttgt tggcgagaaa tttcttgtgg
    21781 tcgacgcgct tgagatcttt aatcacgttc agcaggacgc ctaccaaaaa gacaatcacc
    21841 agaatccacc aatatttagc cagccagtcc atgcttattt cctcttgcag aaccactcat
    21901 caggcgacga gctgttccat gatacgttga tacatacggg caagtagctg caggtcggca
    21961 gcgttcacac attcattaat tttatgaata gtggcattga ccggcccgag ttccaccacc
    22021 tgcgccccca tgcgagcaat aaagcgcccg tcggacgttc cgcctgtggt cagtagctgc
    22081 ggtttaattt cattatagtg ctcaaccgcg ttaacgaccg catccaccag tttaccgcgc
    22141 gcggtcaaaa atggctgccc ggaaagccac caatccaccg tatagcgcag ttgatgtttt
    22201 tcaagcaggg caagcacctg cgctttgatc atctcatcag tcagttcggt gctgaagcgg
    22261 aagttaaact gcacaaacag ttcacccgga ataacgttgt tactgcccgt tcccgcctga
    22321 atattggcaa tctgcatact ggtcgccggg aagaattcat tgccctgatc ccactcaata
    22381 gccactaatt cattaaggaa aggtgctgcg cgatgtactg gattgtcagc cagatgtggg
    22441 taggccacat gcccctgcac gccatgaatg gtaaggttgc aggttaacga tccgcgacga
    22501 ccatttttca ccacatcacc aaccacttcg atactcgacg gttcgccgac cagacagtaa
    22561 tcgagacgct cattacgcgc cattaacgct tcgacgactt ttaccgtacc gttgtgggca
    22621 ctggcttctt catcagaggt gatcagaaat gccagtcgtc ccgtatggtt gggatgttgt
    22681 gcgacaaaac gttctgctgc taccaccatc gccgccagcg agcctttcat atctgccgca
    22741 ccgcgcccga ataacatgcc atcacgaatg gtgggttcaa acggcggatt gatccaacga
    22801 tcggcgtcgc caggcggcac cacgtcggta tgcccggcaa aggccaatgt ttcaccttgc
    22861 ccacgccatg cccaaaaatt ctgcgtatcg gcaaagtcca tgcgttcaac ggtaaaaccg
    22921 atcgcctgca aacgatcaat caacaaagcc tggcatcctg cgtcatcagg actcagggaa
    22981 gggcggcgaa taagctgttg tgtcagctca ataaccgggc acgacataga ctacacctca
    23041 tggaaaaatt gctgataact ggaatcactg aaacccagca gcataggctt accgggcgcg
    23101 cagagcaatg gacgtttgat aattgcaggc atttcagtca ttaatgccgc cgctgaggcc
    23161 gcatcggtga ttttattgcg ggtggtttcg tccagtttac gccaggttgt accacgggtg
    23221 ttgagtaacg cctcccaacc taattcgttg ataaaaccgt tcaataattc gctgtccagc
    23281 ccatcgacgc ggtaatcatg aaaacgatag tcgatgttat tggcttccag ccaacggcga
    23341 gcctttttaa tggtgtcaca atttttgata ccgtaaagtg taaccataat gaatcctttt
    23401 tcgatttatg agaataaata ctcatttaag ggtgaaaaca ttttgcctgc aaatgattat
    23461 caggaaatat aacattatcg aagtattcaa attaacaact attaaataat attaaacatt
    23521 tttaacgtaa acattaagaa tattcatctt gttatctgtc tgtattttaa cctgaattaa
    23581 atcagtatta gatgccgtat gaatatctcg aaatgttgct gtgtatcaca tacaaatcat
    23641 caccgcagga ccatagtatt tacataagat ttgtcccccc ggaggtagtc atcgcagcaa
    23701 tcgagtaaat tagcttcacc acggtatatt gtttccgtag aatacccata attataagag
    23761 aggttgttat gattgaacgt gaactgggga actggaaaga ctttatcgaa gttatgcttc
    23821 gtaagtaatt cctgagacaa agtataaaca gcaagaaccc gctgagcagg ttcttaatcg
    23881 gaaagttgaa aaaggcgaca cattggcatg tcgccttttt tattgcttat tccgggcgcg
    23941 gctttagcgg gaagcggcgg cgcaccagca caaagaacag cggcacgaag taaatagcca
    24001 gaatagtggc tgaaatcatc ccgcccatta cgccagtacc caccgcatgc tgaccaccgg
    24061 aaccggcacc cgtgctggtt gccattggca atacgccgaa gataaatgcc agcgaggtca
    24121 tcagaatcgg gcgtaaacgc tgacggcagg cgtggagcgt cgcttcaaac aggtcgtggc
    24181 ctttttggtt catctcgtta gcaaactcga cgatcaggat ggcgtttttc gccgataaac
    24241 caatgaccgt taacaggccc acctggaagt aaacgtcgtt ttccagcccg cgcatccagg
    24301 ttgccagcag cgcgccgatt acccccagcg gcacgaccag cattaccgag aacggcaccg
    24361 accagctttc atacaacgca gccagacaca ggaataccac cagcaaggaa atggcgtaca
    24421 gcgccggagc ctgcgcgccg gaaagtcgct cctgatacga catcgccgtc cactccagac
    24481 caaagccgtt tggcagctgc ttcactaacg attccataat atccatcgcc gtaccggtac
    24541 tgacccccgg tgcggcttcc ccaacaatct caaccgcaga ataaccgtta tagcgttcca
    24601 gacgcggcga gcctgtttcc cagcgtgagg tcgcgaaagc agagaagggc accatgccgc
    24661 catctttatt tcggacatac cagagattga tgtcatctgg cagcatgcga tacggcgcag
    24721 ctgcctgcac atagactttc ttcacgcgac cgcgatccat aaagtcattc acatagctcg
    24781 aaccccaggc ggtttgcagt gtgtcgttaa tatcgtcgat agcaacgccc agcgcctgag
    24841 ctttacgctg gtcgatatca atctgcaact gcggactgtc gtcgaggccg ttatggcgca
    24901 cacgggttag ctccgggttt tccgccgcca gcgccagcaa ctggttacgt gctgccatca
    24961 gcgcatcgtg acctgctcca gcgtggtcct gcaactccat atcaaaaccc gcagaactac
    25021 caagaccgct aattgctggc gggctgctgg cgataacgcg agcttcttta atcttgttaa
    25081 acgcctttgt tgcacgctcg ataatggcaa acgaggtgcc ggtcttactg tcgcgttcgc
    25141 tccagtcttt caggcggata aacattcgcg ccacgttttg cccgttaccc ccagggccag
    25201 aaccaacggt ggcaaacacc gacatgatgt tgtctttttc atgggtgaag taatatttct
    25261 cgatttgctc aacgactttc agggtctgtt gttgggttga accgctgggc aattgtaccg
    25321 aggtagtaaa catgccacgg tcttccagcg gtaagaacga cgtcggcaaa cgcaggaaca
    25381 ggaacaccat gccgccaagc agcaggacat aaatcacaat ccagcgcagg ctgcggtgga
    25441 gaattttcgc cacccctttt tcgtagcgtt cggcgttgcg gttaaacatc tggttaaacc
    25501 aggcaaaaaa gcctttttgc ccgtgatgct cacctttttt cagtggctta agtagtgtgg
    25561 cacacagagc cggagtgagg atcatcgcta ccagtactga cagcaccatc gccgcaacaa
    25621 tggtaataga gaactggcga tagatggcac cggtggtgcc gccgaagaag gccattggta
    25681 caaataccgc cgaaagcacc atcgcaatcc cgaccagtgc cccctggatc tgccccatcg
    25741 atttacgtgt ggcttcgcga ggagtgagtc cttcctcact cataatacgt tcgacgtttt
    25801 ccaccaccac gattgcgtca tccaccagca gaccgatcgc cagtaccatc gcgaacatgg
    25861 ttaaggtgtt gacgctgtaa ccgaaggcgt aaagtacgga gaaggttccc atcaacacca
    25921 ccggcacggc gatagtgggt atcagcgtgg cgcggaagtt ttgcaggaac agatacataa
    25981 cgaggaaaac cagggcgata gcttccagca gcgttttcac cacgtcttca atggaggctt
    26041 taacaaacga ggtggtttca tacgccacct tgtattccag tccgtgcggg aagtactgcg
    26101 ccagctcgtc gagacgattg agcaccagct ccgctgtcgc catttcgttt gcgccggagg
    26161 ccagttttac ccccagcccg gaggctggct taccattgaa gcggctaaga taatcgtatt
    26221 tctccgcccc catttcgacg gtggcgacat cgcccagcct tacctctgag ccgtcttgat
    26281 tcacccgcaa ggtgatatcg cggaactgtt ctggtgtttg cagcagtgac tgggcgttaa
    26341 tggtggcgtt gagcgcctgc ttatcgacgg aaggtgtacc accaagttgc ccaaccgcaa
    26401 tctgcgcgtt ctgtgactca atggcatcag tgacatcttt agccgtcatc tggaaactgt
    26461 tgagtttcgc cgggtccagc cagatacgca tggaatattg cgaaccatag gcatcgatat
    26521 ccccgacgcc attcacgcgg cttaacgggt cctgaatatt actggcaaca taatcggcaa
    26581 tatcctgttt atccatcgaa ccatcggtag agacgaaagc aatggtcaga atattggtat
    26641 cgccggtttt acgcaccgtc acgccctgat tttgcaccgc ctgcggtaac tttcgcatgg
    26701 ctgattgcag ctggttttgt acttgctgca cggcttcgtc cggatcggtg cctgctttaa
    26761 aacttaaagt gacagatgcc tgaccgatgc cactgctctg agatgacata tacatgagat
    26821 tatcgaggcc ggtcatattt tgctcgataa cctgggtcac ggtgttttcc agcgtctggg
    26881 ccgatgcgcc gggatagtta gcggtcactc gcacattcgg tggcgcgaga tcggggtatt
    26941 gttcaacggg caatgaaaaa atcgccaggg tccctgtcag acacaacagg attgccagca
    27001 cccaggcaaa aatggggcga tcaataaaga aattcgccat taaaagagga cctcgtgttt
    27061 ctgcgtatcg ttgtaggctt gacttgcttc actgtagcgg caaggtacga gccaatcgtg
    27121 gagaaaaaaa ggagttaatg taaattatca tgccctttta tactgaacct taagtgcaag
    27181 tattctttgg tcagtaggag gcattacatc aactgacttt cctcaccctc cgcagagcga
    27241 aagctaatac tcactaacgt gccgccgccg gaaggttgtg aaaaagtcag cgtcccaccc
    27301 agccgttccg cgcgttcgcg catgatattc agaccataat gaccttcggg ttctttcggt
    27361 tcaccgatac caatcccgtt atcacggata taaaccgtgt gattgccgtc cggcgcggtg
    27421 acgcaactga cggcgatttc gctggcgttg gcgtgcttca tcgcattcag caccgcttcg
    27481 cgaataattt gcaacaaatg cacctgcatt tgcgcatcca gcgccagggt tggcagacgg
    27541 cagtcgaggg tcagtttggc gctggtttga ttttgtaacg tatccagcat ttccctcaac
    27601 gcggagggga gatccgcctg ctgcagcgtc aggcggaaag tagtcaacag ctcgcgtaac
    27661 tgccgataag catcattcaa cgcctgggaa aaatcggcca tgatactttg tgcggtggcg
    27721 ttatcttccg gtatcgaacg cttcagtaac gtcaactgga tacgtaagta agaaagtacc
    27781 tgagccagcg agtcgtgcaa ttcgcgggcg atggttgcac gttcttccat caacaataac
    27841 tgctgaaaat gcttttgcgc ctgattaaag tacaaaccgc gtcccagcat cgacgaaacg
    27901 ctgttaagca gcggttctga tgatgaaacg tgactatttt gccagtgcag ttcgccgtaa
    27961 accgtctctt gcattgtcac cggtaaaatc tgcatcggca attccgggtt tggttgccct
    28021 tcgctaatcc gccagttttc accgacattt aactccagat attcagccgc ttcattgtcg
    28081 cgaacaatct gcaaaatatg gcggaaacaa tgcacatcaa tctggcttgt gttcagcgcc
    28141 tgcgaacatt gatacaacac ctccagacga cgcttggcct cgtggagatc gcgggtcttt
    28201 tcttctactg acgcctccag cgaaaggtac aatttatgca gctcgctcga catctggtta
    28261 aaggtttttg caagcagacc aagctcattc ggcagactgg tatccagcgg cggcgagtcg
    28321 aactgcccgt gttcaatacg ttgactggcg gtaaccagct gattcagcgg ggcaaccacc
    28381 tgatggcgta tacggcgcaa agtaaaaaag accagcgtga aaataccgat accgccagcc
    28441 agggaaatcg ccaccaccag cagcattttg cgttcagcgt agtgctgtaa agccagcacg
    28501 aacaggtcta tctgattaac ataattatta atattggcct gataccacgg caaatcgccc
    28561 ttgctgagcc gattattcat ctccagccag ttggcattca gatgcgcata gcgagttttt
    28621 actgcttctg gcacatacca gacgttgaga ttggttaata ccgatgaatg cagtgcctgc
    28681 tgaaacaact ggcgatgtgc attgagttgt ggactgccac tttgcaagtc gtagcccagg
    28741 cggtaactct gcatacgcag cgatccggca atattgatag cctcagcgtc gcgcaaactg
    28801 ctcgccagag ttagcagagc gatacccgtg gaaagaatcg acagcagcac aatgtaaaaa
    28861 aaggcccggg ccagactggc cgagacgggt cgtttaacaa tcacacgcgt cttctccaca
    28921 aaaaatcatt aatgttccag ttcgctaaaa cgcaatttag tcagtgatgg gattatttga
    28981 tctgccacag tttatagcga aatagttact tattctgggc acatgaacat tgccgattcc
    29041 cctgtaagtc gggtaataac aacactcata taaagaataa ggtttttaca accaaaaaag
    29101 aaggtcgtta tgaatcgttt tattatggcc aacagtcagc aatgtctggg ttgtcatgct
    29161 tgtgaaatcg cctgtgtcat ggctcacaat gatgagcaac atgtcctgag ccaacaccat
    29221 tttcatcccc gaattacggt tatcaaacat cagcagcaac gtagtgcagt gacctgtcac
    29281 cattgtgaag atgcgccctg cgcccgtagc tgccctaatg gcgcaatcag ccacgttgat
    29341 gacagcattc aggtcaatca gcaaaagtgt attggctgta aatcctgcgt ggtggcctgt
    29401 ccgtttggta cgatgcaaat cgtcctgaca cccgtcgcgg caggaaaagt aaaagccacg
    29461 gcgcataaat gcgacctttg tgcagggcgc gaaaacggtc ctgcctgtgt cgagaattgc
    29521 ccggcggacg cgctgcaact ggtcactgac gtcgcactct ccggcatggc gaaatcccgc
    29581 cgcttgcgca ccgcacgtca ggagcatcaa ccgtggcatg ccagtaccgc tgcacaagaa
    29641 atgccggtaa tgagtaaagt cgaacaaatg caggcaacgc ccgcgcgtgg cgagccggat
    29701 aaactggcga ttgaagcgcg caaaaccggt tttgatgaaa tttatctgcc attccgtgcc
    29761 gatcaggcac aacgggaagc ctcgcgctgc cttaaatgcg gcgagcacag cgtttgtgaa
    29821 tggacctgcc cgctgcataa ccatattccg cagtggattg aactggtgaa agccggaaac
    29881 atcgacgccg ccgtcgagct ttctcacctg accaacaccc tgccggaaat taccggacgc
    29941 gtttgtccgc aggaccgttt gtgtgaaggt gcctgtacta ttcgcgatga gcacggcgcg
    30001 gtaactatcg gcaacattga acgctacatt tcagatcagg cgttggcgaa aggttggcgt
    30061 cctgacttaa gccatgtcac caaagtggac aagcgggtgg cgattatcgg tgcgggtccg
    30121 gcagggctgg cctgtgcgga tgttctgacc cgcaatggcg tggcggtgac ggtgtacgat
    30181 cgccatccag aaattggtgg cttgctcact ttcggcattc cttctttcaa actggataaa
    30241 tccctgctgg cacgccgtcg ggaaatcttc agcgcgatgg ggattcactt cgaactcaat
    30301 tgtgaagtgg gtaaagatgt ctctttggat tcgcttttgg aacaatacga cgcggtcttc
    30361 gttggcgtag gcacttaccg ttccatgaaa gcgggtttac ccaatgaaga tgcgccgggc
    30421 gtttatgacg cgctgccgtt cctcatcgcc aacactaaac aggtgatggg actcgaagag
    30481 ctaccggaag agccgtttat caataccgcc ggactcaacg tcgtggtact gggcggcggc
    30541 gacaccgcga tggactgtgt gcgtaccgcg ctgcgccacg gcgcgagtaa cgtcacctgc
    30601 gcttatcgtc gtgatgaagc taacatgcca ggctcgaaga aagaggtgaa gaacgcccgc
    30661 gaagaggggg ccaacttcga atttaacgtc cagccggtgg cgcttgagct gaatgagcaa
    30721 ggtcacgttt gcggaattcg tttcctgcgc acgcgtcttg gcgagccgga tgcccagggg
    30781 cgccggcgac ttgtaccgat tgagggcagt gaatttgtca tgccagccga tgcggtgatt
    30841 atggcgtttg gcttcaatcc acacgggatg ccgtggctgg agtcgcacgg tgtaacggta
    30901 gacaaatggg gccgcatcat cgcggatgtg gaaagccagt accgttacca gaccaccaat
    30961 ccgaaaatct tcgccggtgg cgacgccgtg cgtggtgcgg atctggtggt taccgcaatg
    31021 gcagaaggac gtcatgcggc acaggggatt attgactggc tgggggtaaa atcagtcaaa
    31081 tctcactgat agcctgcgca gacaaacccg acttcacagc gtaatataat tgttcatttc
    31141 gcgctgtgga gtcggtatga cgcaacaaat caccctcatt aaagacaaaa ttctctccga
    31201 taactatttc accctgcaca acattactta cgatctcacg cgcaaagatg gctaagttat
    31261 ccgccataaa cgtgaagttt acgatcgcgg taatggtgcg acgatcctcc tgtacaacgc
    31321 gaagaaaaag accgtggttc tgattcgtca gttccgtgtc gctacctggg ttaatggcaa
    31381 tgaaagcggg cagctgattg aaacctgcgc cgggctgctg gataacgacg aaccggaagt
    31441 gtgcattcgt aaagaagcga ttgaagagac gggctatgaa gtgggcgaag tgcgcaaatt
    31501 atttgaactg tatatgtcgc ctggcggtgt gactgagcta atccactttt ttatcgccga
    31561 atacagtgac aatcagcgcg ctaacgccgg tggcggtgtt gaagatgaag atattgaagt
    31621 gctcgagctg ccgttcagcc aggcgctgga gatgatcaaa accggcgaga tacgtgacgg
    31681 taagacggtg ttattgctta actatttgca aacgtcacat ttaatggact gaaaaataac
    31741 aataatattt cgttgtttat tattggctca atccgacaaa tctgattgag ccgcgctacc
    31801 tgcgcaacga agatacgact tgtgctgttt gtttgaactt ctggggtcgt accgtccatg
    31861 cgctatcgca ttttccttct cttttttttc gctttgttgc cgacgtcttt ggtgtgggcg
    31921 gcactagcgc aacgggcgtt ttccgactgg caggtcacct gtaataacca aaacttctgc
    31981 gtggcgcgca atacgggcga tcataatgga ctggtgatga ccctgagccg cagcgccggg
    32041 gcgcataccg atgccgtttt acgtattgag cgcggcgggt tgaagtcgcc ggatgcgtct
    32101 gaaggagaga tagcgccacg gctgctgtta gatggtgagc cgttggcgtt aagtggtgac
    32161 aagtggcgga tttcaccatg gttattagta accgatgata cggcaaccat caccgcgttt
    32221 ttgcagatga ttcaggaagg gaaggcgatc accttacgcg acggcaatca gaccatttct
    32281 ctgagtggct taaaagcggc gttgttgttt attgatgctc agcaaaagcg cgttggcagt
    32341 gaaaccgcgt ggatcaagaa aggggacgaa ccgccgctca gcgtaccgcc cgcgcctgcg
    32401 ctgaaagagg tcgcggtggt taacccaacg ccgacgccac tctcactcga agaacgcaac
    32461 gatttgctgg attatggaaa ctggcggatg aatggtctgc gctgctcgct tgatccgtta
    32521 cgtcgtgagg tgaatgtcac tgcgctgact gatgataaag cgctgatgat gatcagctgt
    32581 gaggcagggg cctataacac cattgatttg gcatggattg tgtcgcgtaa aaagccgcta
    32641 gcctcgcgcc cggttcggtt gcgtttgccg ttcaacaacg ggcaggagac gaatgaactg
    32701 gaactgatga acgcaacatt tgatgagaaa tcgcgtgaac tggtgacctt agcgaaaggg
    32761 cgcggattaa gcgattgtgg cattcaggcg cgctggcgct ttgacggtca gcgttttcgc
    32821 ctggtgcgtt acgccgcaga acccacctgc gataactggc atgggccaga cgcctggccc
    32881 acgttgtgga tcacccgtta aacaaaatgg cggggacatg taggccggat aagacgctgt
    32941 aggccggata agacgcgtca gcgtcgcatc cggcaatcag catccggcga tcactatcag
    33001 gcacctttca ctcccagcac cttatgcgct tttgccacaa tattctcggc ggtaaagcca
    33061 aagaacggga acagcttatc cgccggagca gattccccat aacccgtcat cccgacaatt
    33121 gcccctttca gaccaacata cttgtaccag taatcggcaa tacctgcttc caccgccacg
    33181 cgagccgcaa cgttagaagg caacaccgac tcccgatatt cctcatcctg ggcgtcgaaa
    33241 atatcggtcg agggcaggga aaccacgcga acattgcgac cttctcctgc taatttctcc
    33301 gcagcttgca gggtgatttc catctctgaa ccggtggcaa tcagaataat atctggctta
    33361 ccgccgctgt ctttcagcac ataaccgcca cgagtaatct ctttaacctg atccggcgta
    33421 cgttccacct gggccagatt ctgccttgag aggatcagtg ccgtcggtcc gttgtggcgc
    33481 tcaaccgcca gcttccagcc caccgccgct tccacctgat cgcacggtcg ccaggtgctg
    33541 aaatttggcg ttaagcgcag gctggccagt tgctcaacag cctggtgcgt cggaccatct
    33601 tcgcccaggc caattgagtc gtgggtataa accataatct gccgcgcttt catcagtgcc
    33661 gccatccgcg cggcgttacg ggcgtattca acaaacatca ggaacgtcgc ggtatacggc
    33721 acaaagccgc cgtggtgcgc gatgccgttg gcgatagcgg tcatgccaaa ttcacgcacc
    33781 ccgtagtgaa tgtagttgcc cgccggatct tccttcagcg aaacagaacc tttccagatg
    33841 gtcaggttgc tgggagccag atccgccgaa ccgccgagca gctccggtag caaggggcca
    33901 taagcgttaa gcgtattttg cgaagcctta cgggtggcga ttttcgccgg attcgcctgc
    33961 aactcattga tatacttctg agtcgttttc tcccagtcct tcggtaaacc accgctcatc
    34021 cgacgggtaa actcttctgc cagttgcgga tgagcctttt tataggcggc aaacttctca
    34081 ttccacctct gctgcgcttt ttcgcctttt tcgcgggcat cccaggcgtg ataaatatct
    34141 ttagggatct caaatggcgg atggtgccag cccagttttt gccgtgccag cgccacttct
    34201 tcttccccca gcggtgcgcc gtgcgcctct tccttacctg ctttattcgg cgatccaaag
    34261 ccaatcaccg tacgacagat aatcagcgac ggcttatctt tcacgctttg cgcttcaagg
    34321 atcgcttcct tcaccgcctg cggatcgtga ccgtcgattt catggatcac atgccagtga
    34381 taggcttcaa aacgttttgc cgtatcgtcg gtaaaccagc cttctgtttc accgtcgatg
    34441 gaaataccgt tgtgatcgta aaaaccaatc agcttgccca gtcccagcgt gcctgccagc
    34501 gaacagactt cgtgggaaat accttccatc aggcagccgt cgcccataaa cacataggtg
    34561 aagtgatcga cgatctcatg gtctggctgg ttaaactgcg ccgccagtgt acgctctgct
    34621 atcgccagcc cgacggcgtt cgccaaacct tgtccaagtg gaccggtggt ggtttcaacg
    34681 cctggcgtat aaccaatctc cgggtggcct ggggttttcg aatgcagttg acgaaagttc
    34741 ttcagctctt ccagcggcag gtcgtaaccg gtcagatgca gcaaactgta gagcagcatc
    34801 gacgcgtgac cgttggaaag aataaagcgg tcgcgatcat accaggttgg gtcggtaggg
    34861 ttatgtttaa gaaaatcgtt ccacagcact tcggcaatat cagccatgcc catcggcgcg
    34921 ccgggatgac cagaattggc tttttgtacc gcatccatac tgagtgcgcg aatcgcattg
    34981 gcaaggtctt ttcgggacat ataacactcc gtggctggtt tatagtttgg cggcaagaag
    35041 atcttccagt ttgcgttgat caacggcgaa cagacgaatg ccttcagaca gtttttctac
    35101 cgccatcgca tactgattgt gctcccaacg gaactccgct tcgctcatgg gagctgggcg
    35161 tgggaacgtc tgagaaggtg ggattaattt acgtaccact ggcgaaactt tttcctgcag
    35221 ctccttcagc aaattcggtg cgatagtcag tcgatcgcag ccggttaagg cgaggatttg
    35281 ttcggtgcga cggaagctcg cgcccatcac aatggtttca tagtggtgtt gcttatagta
    35341 gtcgtagata ttgcgcaccg atttaacgcc cggatcttct tccaccacat acgggtccat
    35401 cggcttgcgt gcctgatacc agtcataaat acgcccgaca aacggcgaaa tcagaaaaac
    35461 gcctgcttcc gcacaggccc gtgcctgtgc aaaagaaaac agcagcgtca ggttgcagtt
    35521 aataccttct ttttccagct cttctgccgc gcgaattcct tcccaggtcg aagccagctt
    35581 gatcagaatg cgtgatttct caacgccttg ttgctgatac aagtccacca gatggcgcgc
    35641 cttctcaata ctcttttctt tatcaaaaga gaggcgtgca tcaacttctg ttgacacgcg
    35701 accgggtacg attttgagga tttcagcacc gaaattgacc gccagtttgt cacacgctgc
    35761 gaccacctgt tgttcctggg tcttgccatt ttttttaccc caggcgatag catcgtctat
    35821 taaatgctca tattgtgata atccggcagc cttgagtaac agcgaaggat tggtggtggc
    35881 atcctgggga tgataatggc gaatggactc aatatcgccg ctgtctgcca cgacagtggt
    35941 gaactgtttg atgccgtcta actcgttcat aggaaatact ccttgaaaag taaagtgtta
    36001 gatgagtgcg ttaattcaca cttctgagaa atttcgctaa acgcatcaaa aaagcatagc
    36061 agacaggcat ggtattgctg gattaagcag gtaacatcag tgttatagga ttattaccaa
    36121 aacattatat taatggtgag agtttggact tgctcaaagt ctgtagactc cggcaaggta
    36181 ataatgtgcg ccacgttgtg ggcagggggt gttgcccaca cactttattt gtgaacgtta
    36241 cgtgaaagga acaaccaaat ggatgaccag ttaaaacaaa gtgcacttga tttccatgaa
    36301 tttccagttc cagggaaaat ccaggtttct ccaaccaagc ctctggcaac gcagcgcgat
    36361 ctggcgctgg cctactcacc cggcgttgcc gcaccttgtc ttgaaatcga aaaagacccg
    36421 ttaaaagcct acaaatatac cgcccgcggt aacctggtgg cggtgatctc taacggtacg
    36481 gcggtgctgg ggttaggcaa cattggcgcg ctggctggca aaccggtgat ggaaggcaag
    36541 ggcgttctgt ttaagaaatt cgccgggatt gatgtatttg acattgaagt tgatgaactc
    36601 gacccggaca aatttattga agttgtcgct gcgctcgaac cgaccttcgg cggaatcaac
    36661 ctcgaagaca ttaaagcgcc agaatgtttc tatattgaac agaaactgcg cgagcggatg
    36721 aatattccgg tattccacga cgatcagcac ggcacggcaa tcatcagcac tgccgccatc
    36781 ctcaacggct tgcgcgtggt ggagaaaaac atctccgacg tgcggatggt ggtttccggc
    36841 gcgggtgccg cagcaatcgc ctgtatgaat ctgctggtag cgctgggtct gcaaaaacat
    36901 aacatcgtgg tttgcgattc aaaaggcgtt atctatcaag gccgtgagcc aaacatggcg
    36961 gaaaccaaag ccgcatatgc ggtggtggat gacggcaaac gtaccctcga tgatgtgatt
    37021 gaaggtgcgg atattttcct gggctgttcc ggcccgaaag tgctgaccca ggaaatggtg
    37081 aagaaaatgg ctcgggcacc aatgattctg gcgctggcga atccggaacc ggaaattctg
    37141 ccgccgctgg cgaaagaagt gcgtccggat gccatcatct gcaccggtcg ttctgactat
    37201 ccaaaccagg tcaacaacgt cctttgcttc ccgttcatct tccgtggcgc gctggacgtt
    37261 ggcgcaaccg ccatcaacga agagatgaaa ctggcggcgg tacgcgcgat tgcagaactg
    37321 gcccatgcgg aacagagcga agtggtggct tcagcgtatg gcgatcagga tctgagcttt
    37381 ggtccggaat acatcattcc aaaaccgttt gatccgcgct tgatcgttaa gatcgctcct
    37441 gcggtcgcga aagcggcgat ggagtcgggc gtggcgacgc gtccgattgc tgatttcgac
    37501 gtctacatcg acaagctgac tgagttcgtt tacaaaacca acctgtttat gaagccgatt
    37561 ttctcccagg ctcgcaaagc gccgaagcgc gtagttctgc cggaagggga agaggcgcgc
    37621 gttctgcatg ccactcagga actggtaacg ctgggactgg cgaaaccgat ccttatcggt
    37681 cgtccgaacg tgatcgaaat gcgcattcag aaactgggct tgcagatcaa agcgggcgtt
    37741 gattttgaga tcgtcaataa cgaatccgat ccgcgcttta aagagtactg gaccgaatac
    37801 ttccagatca tgaagcgtcg cggcgtcact caggaacagg cgcagcgggc gctgatcagt
    37861 aacccgacag tgatcggcgc gatcatggtt cagcgtggcg aagccgatgc aatgatttgc
    37921 ggtacggtgg gtgattatca tgaacatttt agcgtggtga aaaatgtctt tggttatcgc
    37981 gatggcgttc acaccgcagg tgcaatgaac gcgctgctgc tgccgagtgg taacaccttt
    38041 attgccgata cctacgtcaa tgacgaacca gacgcagaag agctggcgga gatcaccttg
    38101 atggcggcag aaactgtccg tcgtttcggt attgaaccgc gcgttgctct gttgtcgcac
    38161 tccaactttg gttcttctga ctgcccgtcg tcgagcaaaa tgcgtcaggc gctggaactg
    38221 gtcagggaac gtgcgccaga actgatgatt gatggtgaaa tgcacggcga tgcggcgctg
    38281 gtggaagcga ttcgcaacga ccgtatgccg gacagccctt tgaaaggttc tgccaatatt
    38341 ctggtgatgc cgaacatgga agctgcccgc attagttaca acttactgcg cgtttccagc
    38401 tcggaaggtg tgactgtcgg tccggtgctg atgggcgtgg cgaaaccggt tcacgtgtta
    38461 acgccgatcg catcggtgcg tcgtatcgtc aacatggtgg cgctggccgt ggttgaagcg
    38521 caaacccaac cgctgtaatt cttttttaac tctcacgctt accctgaata ttcagggtaa
    38581 gcggtttggc tgcaatatat tagtaaagct tattactgag tttgcgaata ataaaaaaaa
    38641 gcagtctata taatatctcg atattatttt atttatattc atgcgttgca tatgaaagtt
    38701 tatgcaccac agcgaatatc tctccattct tagtgatcta cctcaccttt taaacgcgct
    38761 tgccgaattt tgttatttac tctgacgaaa aattgtcacg atacacgaaa gtttttcaca
    38821 ggcggcgact catggataaa gaacgcatca ttcaggaatt tgtgcccggc aaacaggtca
    38881 cactggcgca tctcatcgcg catcccggtg aggaactggc gaagaaaatc ggcgttccgg
    38941 atgcgggcgc aattggcatt atgacgctaa ctcccggcga aacggcgatg atcgtcggtg
    39001 atttagcgct gaaagccgct gatgtgcata tcggttttct cgaccgcttc agcggtgcat
    39061 tggtgattta tggctcggtg ggcgcagtag aagaagcgtt atcacaaacc gtcagcggtt
    39121 taggtcgctt attaaattat acgctttgtg aaatgaccaa aagttaatac gaggcattca
    39181 tgaaacgaat tgcgtttgtg ggtagcgtcg gggcaggaaa aacaacgtta tttaatgcgt
    39241 tgcaggggaa ttataccctc gccagaaaaa cacaggccgt ggaatttaat gataagggcg
    39301 atattgatac gccgggtgaa tattttagcc atccccgctg gtatcacgcc ttaattacca
    39361 cgctacagga tgtagacatg ctgatctatg tccatggcgc aaatgacccg gaaagtcggt
    39421 tgcctgccgg gttgctggat attggcgtca gcaaacggca aatcgccgtc atcagtaaaa
    39481 cggacatgcc agatgctgac gtcgccgcaa cgcgaaagtt attgcttgag acgggtttcg
    39541 aagagccgat tttcgagcta aacagccacg acccgcaaag cgtgcagcaa ctggtggatt
    39601 atctggcatc tctcaccaaa caggaggaag caggtgaaaa aactcatcac agcgaatgat
    39661 attcgtgaag cacacgcgcg cggcgaacag gcaatgtccg tggttctgcg cgccagcatt
    39721 attaccccgg aagcccgtaa agtggcggat ctactgggtt ttaccatcac cgaatgtgat
    39781 gaatcgatcc cggtaacggc gtccgtgccc gccagcgtgc cagccgacaa aaccgaaagc
    39841 cagcgcatcc gcgaaaccat cattgcccag ctgccggaag gccagtttac cgaaagcctg
    39901 gtcgcacagc tgatggaaaa agtgatgaag gaaaaacagt cgctggagca gggcgcaatg
    39961 cagccgagct tcaaatcggt gaccggcaaa ggcggcatca aagtgattga tggcagcagc
    40021 gtcaagtttg gtcgttttga tggcgcacaa ccgcattgcg tgggcttgac tgatcttgtg
    40081 accggagacg acggcagcag catggccgcc ggttttatgc agtgggagaa cgcattcttc
    40141 ccgtggaccc tgaactacga cgaaatcgac atggtgctgg aaggggagct ccatgttcgc
    40201 cacgaaggcg aaaccatgat cgccaaagca ggcgacgtga tgtttatccc gaaaggctcc
    40261 agcatcgaat ttggtacgac atccagcgtg aaattcctgt atgtcgcctg gccggctaac
    40321 tggcaatccc tatgaaagat ttcatcaccg aagcatggct aagagcgaac catacgctca
    40381 gcgaaggcgc agagatccat ctccctgcgg acagtcgcct gacgccctct gcccgggagt
    40441 tgctggaaag ccgccatctg cgcatcaagt ttattgacga gcagggccgc ctgtttgttg
    40501 acgatgaaca gcagcagccg cagcccgttc atgggctgac cagtagcgat gaacatccgc
    40561 aggcgtgctg cgaactgtgc cgccagccgg tggcaaaaaa gccggatacg ctgactcacc
    40621 tgtcggcgga aaaaatggtc gccaaaagcg atccgcgtct gggttttcgc gcggttctcg
    40681 acagcaccat tgcgctggca gtgtggctgc aaattgaact ggcggaaccg tggcagccgt
    40741 ggctggcgga tatccgttcg cgtcttggca acattatgcg cgccgatgcg ctgggggaac
    40801 cgctgggctg ccaggcgatt gtcgggcttt ctgacgaaga tctgcatcga ctttctcacc
    40861 agccgctgcg ctatctcgac cacgatcatc tggtgccgga agccagccac gggcgcgatg
    40921 cggcgttgct caatctgcta cgtaccaaag tgcgggaaac cgaaaccgta gcggcgcagg
    40981 tgtttatcac ccgcagcttt gaagtattac gcccggacat cttgcaggcg ctgaaccgcc
    41041 tttcgagcac ggtctacgta atgatgatcc tgagcgtaac gaagccgccg ctaacggtga
    41101 agcagatcca acaacgactg ggggaaacgc aatgattatt gaacgttgtc gtgaactggc
    41161 gttgcgagcg cccgccagag tggtttttcc ggatgcgtta gatcaacgtg tgctgaaagc
    41221 tgcgcaatat ttacatcaac aaggtctggc aacgcccatt ctggtcgcta atccgtttga
    41281 acttcgtcag tttgcgctca gtcacggcgt ggcgatggac gggctacagg tgattgatcc
    41341 gcatggcaac ctcgcaatgc gggaagaatt tgctcatcgc tggctggccc gcgcgggcga
    41401 aaaaacgccg ccggatgcgc tggaaaaact taccgatccg ctgatgttcg ccgcagcaat
    41461 ggtcagcgcc ggtaaagcgg atgtctgtat cgcgggcaac ctctcttcca cggcgaatgt
    41521 gctgcgtgcc ggattacgta ttatcggctt gcagccaggc tgtaaaacgc tctcatccat
    41581 tttcctgatg ctgccacagt acagcggtcc ggcgttgggc tttgccgatt gcagcgtggt
    41641 gccacagccg acggcggcgc aactggcgga tatcgcgctt gccagtgccg aaacctggcg
    41701 cgccctcacc ggagaagagc cgcgcgtggc gatgctgtcg ttttccagta acggtagcgc
    41761 ccgtcacccc tgcgttgcca acgtccagca ggcgacagaa atcgtccgtg agcgcgcacc
    41821 aaagctggtg gttgatggcg agttgcagtt tgacgccgcc ttcgtgccgg aagtggcggc
    41881 gcaaaaagcg cctgccagcc cgctacaggg caaagccaat gtgatggttt ttccgtcgct
    41941 ggaagccgga aatattggct acaaaatcgc acaacgactc ggcggatatc gtgccgtcgg
    42001 gccattgata caaggacttg ccgcgccgat gcacgatctc tctcgtggtt gtagcgtgca
    42061 ggaaattatc gagctggcgc tggtggcagc tgtgccgcgt cagacagaag tgaaccgcga
    42121 aagcagttta caaacactgg ttgaatgaac ggtcccgttc tggaccctga taaagaggaa
    42181 agcacaatgg aagcattagg aatgatcgaa acccggggcc tggttgcact gattgaggct
    42241 tctgacgcga tggttaaagc tgcgcgcgtc aagctggttg gtgttaagca gattggcggc
    42301 ggcctgtgta ctgccatggt tcgtggtgat gttgcagcct gtaaagccgc gaccgatgca
    42361 ggtgccgcag cggcacagcg tatcggcgag ctggtatctg tacacgttat cccgcgtccg
    42421 cacggcgacc tggaagaagt cttcccgatc ggcctgaaag gcgatagcag caacctgtaa
    42481 tcaccctgtg tgacgtcgat tcgggcgtca cgctctctcc tttttcattt taccttctgc
    42541 ggagggtagg gggagctttt acctgaaata cgccacggag gcgggtatga aactggcagt
    42601 cgtcactgga caaattgttt gtaccgtacg ccatcacgga ctggcgcatg acaaattgct
    42661 gatggtggaa atgattgatc cacaaggtaa tcctgacggg caatgcgccg tcgccatcga
    42721 caatattggc gcgggaaccg gggagtgggt gttactggtg agtggcagtt ccgcccgcca
    42781 ggcgcataaa agcgaaacgt caccggtcga tctgtgcgtg attggcattg tcgatgaggt
    42841 ggtgtctggc ggtcaggtaa ttttccacaa ataaggcaga acatcatgaa tcaacaggat
    42901 attgaacagg tggtgaaagc ggtactgctg aaaatgcaaa gcagtgacac gccgcccgcc
    42961 gccgttcatg agatgggcgt tttcgcgtcc ctggatgacg ccgttgcggc agccaaagtc
    43021 gcccagcaag ggttaaaaag cgtggcaatg cgccagttag ccattgctgc cattcgtgag
    43081 gcaggcgaaa aacacgccag agatttagcg gaacttgccg tcagtgaaac cggcatgggc
    43141 cgcgttgaag ataaatttgc caaaaacgtc gctcaggcgc gcggcacgcc aggcgttgag
    43201 tgtctctctc cgcaggtgct gaccggcgac aacggcctga ctttaattga aaacgcgccc
    43261 tggggcgtgg tggcttcggt aactccgtcc actaacccgg cggcaaccgt aattaataac
    43321 gccatcagcc tgattgccgc gggcaacagc gtcatttttg ccccgcatcc ggcggcgaaa
    43381 aaagtctccc agcgggcgat cacgctgctc aaccaggcga ttgttgccgc aggcgggccg
    43441 gaaaacttac tggttactgt ggcaaatccg gatatcgaaa ccgcgcagcg tttgttcaag
    43501 tttccgggca tcggccttct ggtggtgacc ggcggcgaag cggtagtaga agcggcgcgt
    43561 aaacacacca ataaacgtct gattgccgca ggcgctggca acccgccggt agtggtggat
    43621 gaaaccgccg acctcgcccg tgccgctcag tccatcgtca aaggcgcttc tttcgataac
    43681 aacatcattt gtgccgacga aaaggtactg attgttgttg atagcgtagc cgatgaactg
    43741 atgcgtctga tggaaggcca gcacgcggtg aaactgaccg cagaacaggc gcagcagctg
    43801 caaccggtgt tgctgaaaaa tatcgacgag cgcggaaaag gcaccgtcag ccgtgactgg
    43861 gttggtcgcg atgcggcgaa aatcgcggcg gcaatcggcc tgaacgtgcc gcaagaaacg
    43921 cgtttgctgt ttgtggaaac cactgcagaa catccgtttg ccgtgaccga actgatgatg
    43981 ccggtgctgc ccgtcgtgcg cgtcgccaac gtggcggatg ccattgcgct ggcggtgaaa
    44041 ctggaaggcg gttgccacca cacggcggca atgcactcgc gcaacatcga aaacatgaac
    44101 cagatggcga atgctattga taccagcatt ttcgttaaga acggaccgcg cattgccggg
    44161 ctggggctgg gcggggaagg ctggaccacc atgaccatca ccacgccaac cggtgaaggg
    44221 gtaactagcg tgcgtacgtt tgtccgtctg cgtcgctgtg tattagtcga tgcgtttcgc
    44281 attgtttaag gagtaacaga tggcgcacga cgaacaatgg ctcaccccac gtctgcaaac
    44341 ggcggcaacg ctgtgtaacc agacgcccgc cgcgacggaa tctccgctgt ggctgggggt
    44401 ggatctgggc acctgcgatg tggtgtcgat ggttgtcgac cgcgacggtc agtcggtggc
    44461 ggtatgcctc gactgggccg atgtggtccg cgacggcatc gtctgggatt tcttcggcgc
    44521 tgtcaccatt gttcgtcgcc atctcgacac gctcgagcag caattcggtc gccgttttag
    44581 ccatgcggcg acttcatttc ctccaggtac ggacccgcgt atttccatca acgtgctgga
    44641 gtctgctgga ctggaagtga gccatgtgct ggatgagcca accgcagttg cggatctgct
    44701 gcaactggac aacgccggtg tagtggatat cggcggcggc actaccggca ttgccatcgt
    44761 gaaaaagggc aaggtgacgt actcggcgga tgaagcaacc ggcggacatc acatctctct
    44821 gactctcgcc ggaaatcgcc gtatttcgct ggaagaggcg gagcagtaca agcgcggtca
    44881 cggtgaagag atttggccta cggtgaaacc ggtgtacgaa aaaatggcgg acattgtcgc
    44941 ccggcatatt gaagggcagg gcattactga tttatggctg gcgggcggct cttgtatgca
    45001 accgggcgtg gcggaactgt ttcgcaaaca attcccggcg ttacaggtgc atttaccgca
    45061 gcacagtttg tttatgaccc cgctggcgat cgccagtagt gggcgagaga aagcggaggg
    45121 gctatatgca aaatgaattg cagaccgcgc tctttcaggc gttcgatacc ctgaatctgc
    45181 aacgggtaaa aacatttagc gttccaccgg tgacgctttg cggtccgggc tcggtgagca
    45241 gttgcggaca gcaagcgcaa acgcgtgggc tgaaacatct gttcgtgatg gcagacagct
    45301 ttttgcatca ggcagggatg accgccgggc tgacgcgcag cctggctgtt aaaggcatcg
    45361 ccatgacgct ctggccatgt ccggtgggcg aaccgtgcat caccgacgtg tgtgcagccg
    45421 tggcgcagtt gcgtgagtca ggctgtgatg gggtgatcgc atttggcggc ggctcggtgc
    45481 tggatgcggc gaaagccgtg gcgttgctgg tgacgaaccc ggatagcacg ctggcagaga
    45541 tgtcagaaac cagcgttctg caaccgcgct tgccgctgat tgccattcca acgaccgccg
    45601 gaaccggctc tgaaaccacc aatgtaacgg tgattatcga cgcggtgagc gggcgcaagc
    45661 aggtgttagc ccatgcctcg ctgatgccgg atgtggcgat tctcgacgcc gcgttgaccg
    45721 aaggtgtgcc gtcgcatgtc acggcgatga ccggcattga tgcgttaacc catgccattg
    45781 aagcatacag cgccctgaac gctacaccgt ttaccgacag tctggcgatt ggtgccattg
    45841 cgatgattgg caaatcgctg ccgaaagcgg tgggctacgg tcacgacctt gccgcgcgcg
    45901 agagcatgtt gctggcttca tgtatggcgg gaatggcgtt ttccagtgcg ggtcttgggt
    45961 tgtgccacgc gatggcgcat cagccgggcg cggcgctgca tattccacac ggtctggcga
    46021 acgccatgtt gctgccaacg gtgatggaat ttaaccggat ggtttgtcgt gaacgcttta
    46081 gtcagattgg tcgggcactg cgaactaaaa aatccgacga tcgtgacgct attaacgcgg
    46141 taagtgagct gattgcggaa gttgggattg gtaaacgact gggcgatgtt ggtgcgacat
    46201 ctgcgcatta cggcgcatgg gcgcaggccg cgctggaaga tatttgtctg cgcagtaacc
    46261 cgcgtaccgc cagcctggaa cagattgtcg gactgtacgc ggcggcgcaa taaatgccgg
    46321 atgcgacgct tgccgcgtct tatccagcct acgggattgc acatgtaggg cggataaggc
    46381 gttcacgccg catccgccag tggcgcggtg caattgccgg atgcgatgct tgccgcgtct
    46441 tatccggcct acgggattgc acatgtaggg cggataaggc gttcacgccg catccgccag
    46501 tggcgcggtg caattgccgg atgcgacgct tgccgcgtct gatccggcct tcgggattgc
    46561 acatgtaggg cggataaggc gtttacgccg catccgccaa taaataacaa ctaacaggga
    46621 gtaaaggcga tgggaattaa cgaaatcatc atgtacatca tgatgttctt tatgctgata
    46681 gctgccgtag acaggatcct gtcgcagttc ggcggttctg ctcgtttcct cggtaagttc
    46741 ggtaaaagta tcgaaggatc aggcggtcag ttcgaagaag gctttatggc aatgggcgct
    46801 ctgggtctgg cgatggtcgg tatgaccgcg ctggcaccgg tactggctca cgtactcggg
    46861 ccggtaatta tcccggttta cgaaatgctc ggcgcaaacc cgtcaatgtt cgccggaacg
    46921 ctgctggcgt gcgatatggg cggcttcttc ctcgccaaag agctggcggg cggcgacgta
    46981 gcagcgtggc tatactctgg gttaattctt gggtcgatga tggggccaac gattgtgttt
    47041 tccattccgg tggcgctcgg cattatcgaa ccttctgacc gtcgttatct ggcgctcggc
    47101 gtgctggcgg gcattgtgac cattccgatt ggctgtattg ctggtggtct ggttgctatg
    47161 tactccggtg tgcagatcaa cggtcagccg gtggaattca ctttcgccct gatcctgatg
    47221 aacatgatcc cggtgatcat tgttgcgatt ctggtggcgc tggggctgaa attcatcccg
    47281 gaaaaaatga tcaacggctt ccagatcttc gccaaattcc tcgttgcatt gatcaccctc
    47341 ggtcttgctg ccgcggtagt taagtttctg cttggctggg aactgatccc cggtctggac
    47401 cctatcttta tggcccctgg cgataaaccc ggtgaagtga tgcgcgccat tgaagttatc
    47461 ggttctatct cctgcgttct gttaggggcg tatccaatgg tgctgctgct gacccgctgg
    47521 tttgaaaaac cgctgatgag cgtcggtaaa gtgctgaata tgaacaacat cgcggcagcc
    47581 ggcatggtgg caacgcttgc caacaacatc ccgatgttcg gcatgatgaa gcagatggat
    47641 acccgcggca aagtcatcaa ctgcgccttc gccgtttccg ctgctttcgc cctgggcgac
    47701 cacttaggct tcgccgctgc caacatgaac gccatgatct tcccgatgat tgtcggcaag
    47761 ttgatcggcg gcgtaacggc gattggcgtg gcgatgatgc tggtgccaaa agaagacgcg
    47821 accacggcta aaaccgaagc ggaggcacaa tcgtgaacac tcgccagcta ttgagcgtcg
    47881 gtatcgatat cggcaccacc accacccagg tgattttctc ccacctggag ctggttaacc
    47941 gtgcggcggt gtcgcaggtg ccgcgctacg aattcattaa acgcgaaatt agctggcaaa
    48001 gcccggtgtt ctttacccct gtcgataaac agggtggttt aaaagaagcg gaactgaaaa
    48061 ccttaatcct cgagcaatat caggctgcgg gcattgcgcc ggaaagcgtt gattctggtg
    48121 ccatcatcat caccggtgaa agcgcgaaaa cccgcaatgc tcgtccggcg gtgatggtgc
    48181 tctcccagtc gctgggcgat tttgtcgttg ccagcgccgg gccgcacctc gaatccgtca
    48241 ttgccggtca cggtgccggg gcacaaaccc tttctgaaca acggctgtgt cgggtactga
    48301 atattgacat cggcggtggc actgcgaact acgccctgtt cgatgccgga aaaatcagcg
    48361 gcactgcctg cctcaacgtc ggtggtcgcc tgctggaaac cgacagccag gggcgcgtgg
    48421 tttacgctca taaaccgggg cagatgattg tggatgagtg cttcggcgca ggcaccgacg
    48481 cccgttcgct gaccggcgcg cagctggtgc aggttacccg gcggatggca gaactgattg
    48541 tcgaagtgat tgacggaacg ctctcgccgc tcgcgcaggc attgatgcaa accggtttgc
    48601 tgcccgcagg tgttacgccc gaaatcatta cgctttctgg cggcgtgggc gaatgttatc
    48661 gccaccagcc cgccgacccg ttctgttttg ccgatattgg cccgctgctg gcaacggcgc
    48721 tgcatgacca tccgcgcctg cgtgagatga acgtacagtt tccggcgcaa accgtgcgcg
    48781 ccacggtgat tggcgcgggc gcgcataccc tttcgctctc tggcagcaca atctggcttg
    48841 agggcgtaca actgccgctg cgcaatttgc cggtggcgat cccgattgat gaaacggatc
    48901 tggtgagtgc ctggcaacag gcgctgcttc agctggatct tgatcccaaa actgacgcgt
    48961 acgtgctggc gcttcccgcc tcgctgcctg tgcgttacgc cgcggtactg acggtcatca
    49021 acgcgctggt cgatttcgtc gcgcgttttc caaatccgca tcccctgctg gtggtggccg
    49081 ggcaggactt tggtaaagct ctgggcatgt tgttgcgccc acagctacaa caactcccgt
    49141 tggcagtcat tgacgaagtg attgtccgcg cgggggacta tatcgacatt ggtacgcctc
    49201 tttttggcgg atcggttgtg ccggtgacgg tgaaatcact cgcatttcct tcctgaggga
    49261 acgacttatg aaactaaaga ccacattgtt cggcaatgta tatcagttta aggatgtaaa
    49321 agaggtgctg gctaaagcca acgaactgcg ttcgggggat gtgctggcgg gcgttgcagc
    49381 ggcaagctca caggagcgcg tggcggcaaa gcaggtgttg tcggaaatga ccgtagcgga
    49441 catccgcaat aatccggtga ttgcctatga agatgactgc gtgacgcggc tgattcagga
    49501 cgatgttaac gaaacggcct acaaccagat taaaaacagg agcatcagcg aactgcgtga
    49561 gtatgtgctg agcgatgaaa ccagcgtgga cgacattgcc tttacccgca aagggctgac
    49621 ctcggaagtg gtcgcggcgg tagcgaagat ttgctccaac gcggacctga tctacggcgc
    49681 gaagaaaatg ccggtaatca aaaaggccaa taccaccatc ggtattccgg gcaccttcag
    49741 cgcccgtttg cagccaaatg acacccgtga cgacgtgcaa agtatcgctg cacaaatcta
    49801 cgaagggctt tccttcgggg tgggcgatgc ggtgatcggc gttaacccgg taactgacga
    49861 cgtggaaaac ttaagccgcg tgttggacac catttacggc gtgatcgaca aattcaacat
    49921 cccaactcag ggctgcgtac tggcgcacgt caccacccag atcgaagcga ttcgtcgcgg
    49981 cgcgcctggc ggactgattt tccagagtat ctgtggcagc gaaaaagggc tgaaagagtt
    50041 tggcgtggaa ctggcgatgc tcgacgaagc gcgcgcagtg ggtgctgagt tcaaccgtat
    50101 cgccggggaa aactgcctct acttcgaaac cgggcaaggt tcggcgctgt ctgctggcgc
    50161 taacttcggc gcagaccagg tgacgatgga agcacgtaac tatgggctgg cgcgtcatta
    50221 cgatccgttt atcgtcaaca ccgtggtcgg ctttattggg ccggagtatc tctacaacga
    50281 ccgccagatt atccgtgctg gcttagaaga tcactttatg ggcaagctga gcggcatctc
    50341 tatgggctgt gactgctgtt acaccaacca cgctgacgct gaccagaacc tcaacgaaaa
    50401 cctgatgatc ctgctcgcca ccgcaggctg caactacatc atggggatgc cgctgggcga
    50461 tgacatcatg ctcaactatc agaccaccgc attccacgac actgccactg tgcgtcagtt
    50521 actcaacctg cgcccgtcac cggagtttga acgctggctg gaaagcatgg gcattatggc
    50581 aaacggtcgc ctgaccaaac gggcgggcga tccgtcactg ttcttctgat gacgcggaga
    50641 taacacatca tggatcaaaa acagattgaa gaaattgtac gcagcgtgat ggcgtcaatg
    50701 ggacaagcgg cccccgcgcc gtcagaagca aagtgcgcca ccactaactg tgcggcaccg
    50761 gtgacctcgg aaagctgcgc gctggattta ggttctgcgg aagcaaaagc gtggattggt
    50821 gttgaaaacc cgcatcgcgc agacgtatta acagaactgc gccgcagcac cgtggcccgc
    50881 gtttgtaccg gtcgtaccgg tccgcgtcca cgtacgcagg cgctgctgcg tttcctggcc
    50941 gatcactctc gttcgaaaga taccgtactg aaagaagtgc cggaagagtg ggtgaaagcg
    51001 cagggcttgc tggaagtacg ctccgaaatt agcgacaaaa atctctacct gactcgcccg
    51061 gatatgggcc gccgcctgtg tgcagaagct gttgaagcgc tgaaagcaca gtgtgttgct
    51121 aatccggacg tacaggtcgt tatttctgat ggcctgtcaa ctgatgccat caccgtgaac
    51181 tacgaagaga tcctgccgcc gctgatggcg ggcctgaaac aggccgggct gaaagtcggc
    51241 acgccattct ttgttcgtta tggtcgcgtg aagattgaag atcagatcgg tgagatcctc
    51301 ggcgcgaaag tggtgatcct gctggtgggg gaacgtccgg ggttgggtca gtcagaaagc
    51361 ctctcctgtt acgccgttta ctcgccgcgt atggcgacca ccgttgaggc cgatcgcacc
    51421 tgcatctcta acattcacca gggcggcacg ccgccggttg aagccgcagc cgtcattgtg
    51481 gatttggcga aacgtatgct ggagcagaaa gcatccggca tcaacatgac ccgataagga
    51541 ggcatcatgc cagctttaga tttgattcga ccgtcggtaa ccgccatgcg ggtgattgcc
    51601 tccgttaacg ctgaatttgc gcgtgaacta aaattgccgc cgcatattcg tagcctcggg
    51661 ctgatttctg ctgattccga tgacgtcacc tacattgcgg ctgacgaagc gaccaagcag
    51721 gcgatggtcg aagtggtata tggccgctcg ctgtatgcgg gagcagcaca cggaccgtca
    51781 ccgactgccg gtgaagtgct gattatgctt ggtgggccaa acccggcgga agtgcgtgct
    51841 ggtctggatg cgatggttgc gcatattgaa aatggcgctg ctttccagtg ggctaatgac
    51901 gcagaaaaca ccgcattcct ggcacatgta gtttcgcgta ccggttctta tctttcatca
    51961 accgccggga tcacgcttgg cgatccgatg gcgtatctgg tggcaccgcc gctggaagcg
    52021 acctacggca ttgatgcagc gttgaaatct gccgacgtac agctggtgac ctatgtcccg
    52081 ccaccgtctg aaaccaacta ctcggcagca tttttaaccg gtagccaggc cgcgtgtaaa
    52141 gcagcctgta acgcctttac cgatgcagtg ctggaaatcg cgcgtaatcc aatccagcgt
    52201 gcgtaacgga ggttgccgat gatcaatgca ctgggattgc tggaagtgga cggcatggtc
    52261 gccgcgatag atgcagcgga tgccatgctc aaagcagcta acgttcgtct gctcagtcac
    52321 gaagtgcttg accctggtcg cttaacgctg gtggtggaag gcgatctggc ggcgtgtcgt
    52381 gcggcgctgg acgcaggttg tgctgccgcg atgcgtaccg ggcgtgtcat cagccgcaag
    52441 gagatcggtc ggccagacga tgacacccag tggctggtta ctggctttaa ccgccagccg
    52501 aagcaacccg taaaggaacc cgacgcgcca gttatcgtcg cggaatctgc tgacgagttg
    52561 ttggcgctgt taacatcagt acgtcaggga atgacggcag gagaagtggc tgcccacttt
    52621 ggctggccgc tggaaaaagc cagaaatgcg ctcgaacagc tcttttctgc cgggacgtta
    52681 cgtaaacgca gtagtcgtta tcgtctcaag ccccattaac ctgtcggagg tgccgggtgt
    52741 cctacaacac ccggcattaa catcatgaaa aagacccgta cagccaattt gcaccatctt
    52801 tatcatgaac ccttacccga aaacctgaag ctcacgccga aggtcgaagt ggataatgtt
    52861 catcaacgac agacaacgga tgtctatgaa catgctttga cgattaccgc ctggcagcag
    52921 atttacgatc agctgcatcc gggcaagttt catggtgaat ttacggaaat tctgctcgat
    52981 gatattcagg tttttcgtga atacaccggt ctggcgctgc gtcagtcgtg cctggtctgg
    53041 ccgaactcgt tctggtttgg cattccggcg acgcgcggtg agcagggatt tatcggttcg
    53101 caatgtctgg gaagcgcaga aatagcgacg cgccccggtg gaaccgagtt tgaattaagt
    53161 acgccggacg attatacgat cctgggcgta gtgctgtctg aagatgtcat cacccgtcag
    53221 gctaactttt tgcacaatcc ggatcgggta ttacatatgc tgcgtagcca gtcggcgctg
    53281 gaagtgaaag agcagcataa agccgcgctg tggggctttg tccaacaggc gctggcgacg
    53341 ttttgcgaga acccggaaaa tctccatcag cctgcagtgc gaaaagtgct gggggataat
    53401 ttgctaatgg cgatgggggc gatgctggaa gacgcgcaac caatggtgac ggcggaaagc
    53461 atcagtcatc agagttaccg tagattactt tcccgcgccc gtgaatatgt gctggaaaat
    53521 atgtctgagc tggtgacggt gctggacttg tgtaatcaac tgcatgtcag tcgccgcacg
    53581 ctacaaaacg cgtttcacgc tattttaggc attggcccaa acgcgtggct gaaacgcatt
    53641 cgcctgaacg ccgtacgccg cgaactgata agcccgtggt cgcaaagcac aacggtaaaa
    53701 gacgccgcca tgcagtgggg attctggcat ctggggcaat ttgccacgga ttaccagcag
    53761 ctgtttgccg agaagccgtc gttgacgttg catcagcgga tgcgggaatg ggggtgaggg
    53821 agttacaccc aatccctgac cttaataaac tcacttaacg ccgcttctgg gctgccatct
    53881 tttggctgat aatcatactc ccagcgtacc agtggcggca ttgacatcag gatcgactcg
    53941 gtgcgcccgc cagtttgcag gccaaacaga gtgccgcgat cccagaccag attgaactcg
    54001 acataacgac cgcgacggta gagctgaaaa ttgcgctcgc gctcgccgta ggccatcgct
    54061 ttacgtcgct ctacaattgg taaataagcg tcggtgtagc ctttgcctac cgcctgcata
    54121 aaggcaaaac agtggtcgaa atctggcgtg ttcagatcat caaagaacag cccgccaata
    54181 ccgcgctgtt cgttgcgatg tttgaggtag aagtaatcgt cgcaccactt tttgtaacgg
    54241 ggataaacgt cttcaccaaa tggcaggcac aggtcacggg cggtgcggtg ccagtgaatg
    54301 gcgtcttctt caaaaccata gaaaggggtt aaatcgaagc cgccgccaaa ccaccagacg
    54361 ggctctgcac ccggtttttc ggcaataaaa aaacgcacat tcgcgtggct ggtgggaaca
    54421 tacgggttat gcggatgcac taccagtgaa acgcccatcg cctcgaaact gcgcccggca
    54481 agttccgggc gatgagcggt ggcggaagca ggcatcgcct cgccgtggac gtgagaaaag
    54541 ttgacgcctg cctgttcgaa aacaccacca ttacgcaaca cccgactacg cccgccgccg
    54601 ccagcttcgc gctgccaact atcttcgaca aattctgcgc catcgacggc ggtcagctgc
    54661 tgacaaatcg tatcctgaag gttgagcaga aactgtttaa cctggtgtgc gtcgggtttc
    54721 ataacttatc gctttttcga atgtgctttc tggttgtcga accagtggaa ataactgatc
    54781 acgccttcag caatcgctgt ggcgattttc tgacgaaacg ccgccgtgcc taacagccgc
    54841 tcttcttccg ggttggtgat aaacgaggtt tccaccagca ccgaaggaac cgacggcgat
    54901 ttcaacacca caaacgcggc ttgttcggtg ttgcggctgt gcagtttatg caccggctta
    54961 atctttttca gaatatgcga gccaagcgtc aggctgtttt taatagtatc ggtttgcacc
    55021 agatcaaaca gcacttgctg caataggtga tccttgtcag tcgccttttt accggcaact
    55081 tcatcggcgc ggttttcgcg ttcagacagg tatttcgcca ttgcgctact tgccccacgg
    55141 ttagagaggg caaataccga agcaccggca gctttcgggt tggtaaagcc atcggcatga
    55201 attgacataa acagatctgc gccatgttta tgggcgattt caacgcgatc gtaaagtggg
    55261 ataaacgtat cgccagaacg cgttaaacgc gcatcaatcc catgattacg caaaatggaa
    55321 cggacgtttt tagcaatcgc cagcaccaca tgtttttctt tcgaaccgtt gcgtccgatc
    55381 gctccggtat caattccgcc gtggcctgga tcgagaacaa cgacacgttt gccgccagat
    55441 tttttggctt tcggcttgct gtgtccgttg ctggttttta aaggttcgtc tttggcgatg
    55501 gcttgcgaca ttcctggcaa cgtcagggca gccaaaccgg ctttcagcac ctggcggcgc
    55561 gaagtgagtg tttttagtgg tttaaaagtg ctcatacggc ctgagttgta ataataaagt
    55621 tccagatgtt atatcgtatc gcgtaatccg ttacgacggt ttgatgttga gaattgtttt
    55681 acttttcatt tcaatagttg acaactcccc attatgccac tttttcaacc gattttcgtc
    55741 agatattggc taattcgacc gttcacgcca taatcagcct tttaacccgc aaaaaggtaa
    55801 cgaataccat ggagatacgc gtatttcgcc aggaagattt cgaagaggtc atcacccttt
    55861 gggagcgttg cgacttgctg cgtccgtgga acgatccgga aatggacatc gagcgtaaga
    55921 tgaaccatga cgtcagtttg tttttggtcg ctgaggtaaa cggtgaagtg gtcggaacgg
    55981 tgatgggcgg ttatgacggg catcgcgggt ctgcttatta tcttggcgtg catccagagt
    56041 ttcgtgggcg tgggattgcc aatgcgttgc ttaatcggct ggagaaaaag ctgattgctc
    56101 gtggctgccc gaaaattcag atcaatgtgc cggaagataa cgacatggtg ctcggaatgt
    56161 atgaacgcct gggatatgaa cacgccgacg tgctgagtct gggtaagcgt ttgattgaag
    56221 atgaagagta ctgagttcca tcctgtccat tatgatgcgc acggacgcct gcgtttaccc
    56281 ttgctcttct ggcttgtgct attgcttcag gcgcgaacct gggtgctgtt tgtcattgcc
    56341 ggtgcgtcgc gcgagcaggg tactgcgctg ctaaatctgt tttatcccga tcacgataat
    56401 ttctggctgg ggctgattcc aggcattccg gcggtgctgg cgtttttgtt gagcggtcgg
    56461 cgggctacat ttcctcgcac ttggcatgtg ctctattttc tgttgctgtt ggcgcaggtg
    56521 gttttacttt gctggcaacc gtggctgtgg ctgaacggtg aatccgttag cggtatcggt
    56581 ctggcgctgg tagtggcgga tattgtggcg ttaatctggc tgctgaccaa tcgacgttta
    56641 cgcgcttgtt ttaatgaaga aaaagaataa cggcactttt tggtgaattt gcactccaag
    56701 caacgttatt gaataaccaa aggaagtgac atgaaatcgc tgcgtttaat gttatgcgct
    56761 atgccgttga tgctgaccgg ctgttccacg atgtcgtcag ttaactggtc ggccgctaac
    56821 ccgtggaact ggtttggatc gtccaccaaa gtgagcgagc agggcgtggg tgaattaacg
    56881 gcgtccacac cactgcaaga acaagccatt gctgatgcgc ttgatggcga ttatcgcctg
    56941 cgcagcggaa tgaaaaccgc gaacggcaac gtggtgcgct tttttgaagt gatgaaaggc
    57001 gacaacgtgg cgatggtgat taacggcgat caaggcacga tcagtcgcat tgacgtgctg
    57061 gatagcgata ttcctgctga caccggtgtt aaaatcggta caccgtttag cgacctttac
    57121 agcaaagcat ttggcaattg ccaaaaagct gatggtgatg ataatcgtgc cgtcgaatgt
    57181 aaagccgaag gcagtcaaca tattagctac cagttcagcg gggaatggcg tggtcctgaa
    57241 gggttaatgc cttcggacga taccctgaaa aactggaaag tcagtaaaat tatctggcgg
    57301 cgttaatttg cgtctgaaca aaccgccgcc gcgaaaaaac acgtaaaata gcgctcaaca
    57361 atgccacgga ttgcgtggca ttcttatttt caggaggaac aatgtctcag gttcagagtg
    57421 gcattttgcc agaacattgc cgcgcggcga tttggatcga agccaacgtg aaaggggaag
    57481 ttgacgccct gcgtgcggcc agtaaaacat ttgccgacaa actggcaact tttgaagcga
    57541 aattcccgga cgcgcatctt ggtgcggtgg ttgcctttgg taacaacacc tggcgcgctc
    57601 tgagcggcgg cgttggggca gaagagctga aagattttcc gggctacggt aaaggccttg
    57661 cgccgaccac ccagttcgat gtgttgatcc acattctttc tctgcgtcac gacgtaaact
    57721 tctctgtcgc ccaggcggcg atggaagcct ttggtgactg cattgaagtg aaagaagaga
    57781 tccacggctt ccgttgggtg gaagagcgtg acctgagcgg ctttgttgac ggtacagaaa
    57841 acccggcggg tgaagagacg cgtcgcgaag tggcggttat caaagacggc gtggatgccg
    57901 gcggcagcta tgtgtttgtg cagcgttggg agcacaacct gaagcagctc aaccggatga
    57961 gcgttcacga tcaggagatg atgatcgggc gcaccaaaga ggccaacgaa gagattgacg
    58021 gcgacgaacg tccggaaacc tctcacctca cccgcgttga tctgaaagaa gatggcaaag
    58081 ggctgaagat tgttcgccag agcctgccgt acggcaccgc cagtggcact cacggtctgt
    58141 acttctgcgc ctactgcgcg cgtctgcata acattgagca gcaactgctg agcatgtttg
    58201 gcgataccga tggtaagcgt gatgcgatgt tgcgtttcac caaaccggta accggcggct
    58261 attacttcgc gccgtcgctg gacaagttaa tggcgttgta agcgtttctg attcgatgat
    58321 taaaggccag ccagtaacaa aactgactgg cctttttatt acttttgctt caacgccgca
    58381 tacacctgat caactaccca accataagtt gcaatcggta actgaccgct ttcgaacata
    58441 ttgggatttt tttgtggatc ggcaactggc gaatggggct tgttgcttaa catcacaatc
    58501 gccatatgat tgaccggatc gataacggtt actgtcccgg tccagccagt gtgaccgtag
    58561 gtttgcgggc ttgccagcgt gccaaacgtc ggcgtcatgg tggcattacc attcacgcgc
    58621 cagccgaggc caaaagtggc atcttcctta gagctggtgg tgaacatctt caccgtttcc
    58681 gcactgaaca gctgcacatc gccatagccc ccgccgttca gcatcgtttg cattaacacc
    58741 gcaatatcgc cggtattgga aaacaaacct gcgtgcccgg aaacgccgcc catcgaataa
    58801 aaggcttttt catcgtgcac ctgaccccag agagtggagg tgcggatatt cgggaaatgg
    58861 atcacgccat cgcgggtatt gccgttcaat tccgtggcgg caatttgttg cggtttaaag
    58921 cctttcagca gcgggttaaa taccgtatgt gtcaggccga gcgggcgata aatcgactct
    58981 tcaacatagc ggtcaagcgg ctgaccggta accgactcga cgataaatcc aagcagcata
    59041 taatcgacat cgctgtagat atgtttgctg ccgggctgat attccagcgg cgtgcgcttg
    59101 atcatctcca gcgtctggcc tttatcctgg gaatataacg cgcccgcgac cgctttattc
    59161 gggtattgcg gatctgccgg gaaaccgccg ctgtgatgca gcaggtcaga aatccgcagg
    59221 gtgtttttgc ctttaatggt gtcatttgga ctatcggcaa accccggaat atatttcgca
    59281 atccgatcgt caggatgcag tttgccttcg ctcatcagct tttgcagggc gaagttcgtg
    59341 gcgtacattt tggtgtttga ggccaggtca tacagcgtcc cggtggtggc tttgacaggc
    59401 tgttccatca acacgctgcc atcgtacttt ttcgccgctc cccaggcttt gcgatacaca
    59461 atctgattat ctttaatgat cagcaggttt acgccgggat aaccgacatc aatttgctgg
    59521 ctaatccagc gatccatctg gttaagccgt tcgacgttaa accctgcttt ctctggcgag
    59581 ctttctgtca gaacagggta ttttgcggcg ctgacgctac aactaacagc cagcagagaa
    59641 agatagagca ttgtccgttt catgatatca gtccagattg acgttacggc agccaaagag
    59701 cgtggtgaaa ataaacccgc aaacccatgc caccagaatc ccgccagcat aaaccgccat
    59761 ggccggaagg atgccttgtg cggaagtcat cagcggcagc gctaccagac cagacggccc
    59821 aaaggcgctg tttaagccca tcggcagacc ccatcaggct atcaggccga taaacaaacc
    59881 gcccgccgcg ccgcctaaac aggcggtaac aaacggcttc atgcggggca gggtgacacc
    59941 gtaaatcagc ggttcaccaa cgcccagcag gccgggaata atcgccccgc gtacctgact
    60001 gcgtagcgca ctgtgcggtt gcgcccgcca gtagagtgcc agcgccgcgc ccacctggcc
    60061 cgcgcctgcc attgaaagga tgggaaataa gctgttgaat ccctggctgt ccattaacgc
    60121 gaggtaaaca ggaataaagc cctgatgcac gccaaatacc acggcgatca ggaacagccc
    60181 ggctaaaacc gcacaaccga acggattact gttcaggtgc ataaacagcc atgacatacc
    60241 ttcgaataac cagccaccca gcggcataat gatcaggtac gcaagcgtag cggtgatcag
    60301 cagggtgatt aacgaggtca gcagcatgtc gagatcgtcc gacataaagc ggcgcaccat
    60361 gccttcaatg cgggcgcagg cccacgcggc aatcagcacg ccgataatat tgccgcgcgg
    60421 atcgatgggc agaccaaaga aatcgtgaaa accggcgtag taaccggtgg ttgcagcggg
    60481 gttataaccg agcaaaaaga gtgcggcaat aattgcgcca ttgacgcccg tgccgccaaa
    60541 tgcctgggcg gcgttatagc ccaccagaat caccaggaaa gtgaacaaac ctttgctgaa
    60601 caccttcata aaattcagcg cgtcgggtag cgtgccctga gcatctgccg gaacgtgcat
    60661 caccgtggca attaacgtcg ctatccccag cagcagaccg gcggcaataa aaccggggat
    60721 cagcggcgtg aagatggtgg cgaatttggc gagaaattgt tgcacgccgg aggtttgttt
    60781 ggcttttaac tgacgtttgt tctgggcggc gatttctgcg gcatcctgta ccggtgcctc
    60841 gcccagcagc tcgctcatcg cttttgcggc acgatgcgct ttaccaggcc caaaaacgac
    60901 ctgcacctgg tcgctggtca aaatgacgcc cttcacgcct tccagcgttt tgatattggg
    60961 atcaaccagt gaactgtcat gtacacccag acgcaggcgc gtcatacagt taccacaact
    61021 ggcgatattt cccagtccgc cgacacgggt aagaatggtg ttcagaagtt cactgctgat
    61081 ctctttggcc atgggttatt ccttgtctaa aacctgacga ataaagccgc cgtgttgatc
    61141 caggcgtttt ttagcttctg cggcatcgag atttttcagc accatcacaa tggccgtttt
    61201 acagttgcgc tcgcaggcaa ttaacgccgc ttccgcttgt tcggcattac atccggtggc
    61261 gtttttaacg atattgacct ggcgcacatg cagtttttcg ttggtggcga ccacatcgac
    61321 catcaggttg ccgaacactt tgccggattt aatcatcagc ccggtggaaa gcatattgag
    61381 caccagtttc tgcgctgtgc ctgctttcat tcgcgaagaa ccagtgacca cttcggcacc
    61441 tacaatcggt gtaatggcaa actcggcggt ggtggaaacg gcactccccg gattacagga
    61501 aattcccact gtgcggcaac cgagctggcg ggcgtattcc agtccggcaa tcacatacgg
    61561 cgtacgaccg ctggcggcaa taccgaccac cacatcctgt gccgttaaat ttatattttt
    61621 cagatcgtta atgccgcctt ctcggctatc ttccgtgcct tccaccgcgt gctgaatggc
    61681 atattcgccg ccagcaatca aaccaaccac cagacccggt ttcacgccgt aggtgggcgg
    61741 acattcgctg gcatccagaa tccccagacg accggatgtt cccgcaccga gataaatcag
    61801 acgaccgccg ccgcttacct gggcgtggat aacatcgatc gccgcggcga tatccggcag
    61861 gacgcgctca acggcaagcg gtacggtttt atcttcatcg ttgataatcc ggcacatttc
    61921 cagcgtcgat acgcggtcaa tttcagccga ggcggtgttc gagccttcag taatcatctt
    61981 ttcaagttgc atcggtggtg accttactat ttacgatcaa ataattgcag atgtatggaa
    62041 tcatatatcc cgaattgaca gacttaaagg aatcattgag tctacacttg cagagtgtga
    62101 agagcgtcat aagaacttaa gcggaatttt tgctgccagc ccgggagagt ggaagatgtt
    62161 gtacctgacg aaaatacgca atgcggaaag tgaatttacc gagaacgagc aaaaaattgc
    62221 tgactttctg cgggccaacg tgagtgagct gaaatccgtc tcttcgcgca agatggcgaa
    62281 acagctcggc atcagtcagt cgagcattgt gaaatttgcc cagaagctcg gcgcgcaagg
    62341 atttactgaa ttgcgtatgg cgttaattgg cgaatacagt gccagccggg agaaaacgaa
    62401 tgccacggcg ctgcacctgc acagttcgat taccagtgat gattcgctgg aagtgattgc
    62461 ccgcaaactg aatcgcgaaa aagagctggc gctggagcaa acctgtgcgt tgttcgacta
    62521 cgctcggctg caaaaaatca ttgaggtcat cagtaaagca ccgtttatcc agataacggg
    62581 cctgggcggg tcggcgctgg tggggcgcga tctgtcgttc aaactgatga aaattggcta
    62641 tcgcgtggcc tatgaagccg acacacacgt acaagccacc gtttcccagg cgttgaaaaa
    62701 aggcgatgta caaatcgcta tctcttacag cggcagtaaa aaagagattg tcttgtgcgc
    62761 agaagcggca cgaaagcagg gggcaacggt gattgccatt acttctctgg ctgactctcc
    62821 gctgcggcgg ctggcgcatt tcacgctcga taccgtttcc ggtgaaaccg aatggcgtag
    62881 ctcgtcgatg tctacccgca cggcgcaaaa ttctgttacc gatttactgt ttgttggtct
    62941 tgtgcaactc aatgatgtgg agtcattgaa aatgattcag cgcagcagtg agctaacaca
    63001 acgcctgaaa tgatgcataa agcagcgact ggattgagat tttcctgaat tagtgagctg
    63061 atccgcagca atattttgtt tatcctgtat tttcagaggg aatggagtgt aacgctctgt
    63121 attaacaagg agagcattaa aatgggtaaa ctcacgggca agacagcact gattacgggc
    63181 gcattgcagg gaattggcga aggaattgcc agaacttttg cgcgtcatgg cgcaaaccta
    63241 atcttgctgg atatctcccc tgagatcgaa aagctggcgg acgaactgtg tggtcgtggt
    63301 catcgctgta cggcggttgt cgccgatgtg cgtgacccgg cgtcggtagc cgcagctatc
    63361 aaacgcgcga aggaaaaaga agggcgcatt gatatcctgg tgaataacgc aggcgtttgt
    63421 cgtctgggca gtttcctcga tatgagcgat gacgatcgcg atttccatat tgacatcaat
    63481 attaaaggcg tatggaacgt cacgaaggcg gtgctgccgg agatgattgc ccgcaaagat
    63541 ggtcgcattg tgatgatgtc ttcagtcact ggtgatatgg tggccgatcc tggcgaaacg
    63601 gcgtacgcct taacgaaagc ggcgattgtt ggcctgacaa aatcgctggc ggtggagtac
    63661 gcgcagtctg gtattcgcgt taacgccatt tgcccgggat acgtgcgcac accaatggcg
    63721 gaaagcattg cccgccagtc gaacccggaa gatccagagt cggtgctgac tgaaatggcg
    63781 aaagcaatcc cgctgcgtcg cctcgccgat ccgctggaag tcggcgaact ggcggccttc
    63841 ctcgcatcgg atgaatccag ctatttaacc ggtacacaga atgtgattga tggcggcagc
    63901 acactgccgg agacggttag cgtcggtatc tgattcacct ctgtttcctc cctgcatttg
    63961 tggggaggat ttcgttttaa acccggttct tattcccttt tcaacttcca aatcaccaaa
    64021 cggtatataa aaccgttact cctttcacgt ccgttataaa tatgatggct attagaaagt
    64081 cattaaattt ataagggtgc gcaatggccg ttaacttact gaaaaagaac tcactcgcgc
    64141 tggtcgcttc tctgctgctg gcgggccatg tacaggcaac ggaactgctg aacagttctt
    64201 atgacgtctc ccgcgagctg tttgccgccc tgaacccgcc gtttgagcaa caatggacca
    64261 aagaaaacgg cggcgacaaa ctgacaataa aacaatctca tgccgggtca tcaaaacagg
    64321 cgctggcaat tttgcagggc ttaaaagcag acgttgtcac ttataaccag gtgactgacg
    64381 tacaaatcct gcatgacaaa ggcaagctga tcccggcgga ctggcaaacg cgcctgccaa
    64441 ataatagctc gccattctac tccaccatgg gcttcctggt gcgtaagggc aacccgaaga
    64501 atatccacga ctggaacgac ctggtgcgct ccgatgtgaa gctgattttc ccgaacccga
    64561 aaacgtcggg taacgcgcgt tacacctatc tggcggcatg gggcgcagcg gataaagctg
    64621 acggtggcga taaagccaaa accgaacagt ttatgaccca gttcctgaaa aacgttgaag
    64681 tgttcgatac tggcggtcgt ggcgcgacca ccacttttgc cgagcgcggc ctgggcgatg
    64741 tgctgatcag cttcgagtcg gaagtgaaca acatccgtaa acagtatgaa gcgcagggtt
    64801 ttgaagtggt gattccgaaa accaacattc tggcggaatt cccggtggcg tgggttgata
    64861 aaaacgtgca ggccaacggt acggaaaaag ccgccaaagc ctaccttaac tggctctaca
    64921 gcccgcaggc gcaaaccatc atcaccgact attactaccg cgtgaataac ccggaagtca
    64981 tggataaact gaaagacaaa ttcccgcaga ccgagctgtt ccgcgtggaa gacaaatttg
    65041 gctcctggcc ggaagtgatg aaaacccact tcaccagcgg cggcgagtta gacaagctgt
    65101 tagcggcggg gcgtaattaa tgtttgcagt ttcatcaaga cgcgtgctgc cgggctttac
    65161 cttaagcctc ggcaccagtc tgctgtttgt gtgcctgatt ttgctgctgc cgctctccgc
    65221 gctggtgatg cagctggctc agatgagctg ggcgcagtac tgggaggtga tcaccaaccc
    65281 gcaggtagta gcggcctaca aagtaacgct gctgtcagcg tttgtggcat cgatttttaa
    65341 cggcgttttt ggcctgctga tggcgtggat cttaacccgc tatcgctttc cgggccgcac
    65401 gctgcttgat gcgctgatgg atttaccctt tgcgctgcca acggcagtcg ccggtttaac
    65461 gctagcctcg ctcttttctg taaacggttt ttacggtgaa tggctggcga agtttgatat
    65521 caaagtcacc tatacctggc tggggattgc ggtggcgatg gcctttacca gcattccgtt
    65581 tgtggtacgt accgtgcagc cggtgctgga agagttaggc ccggaatatg aagaagcggc
    65641 agaaacgctt ggcgcaacgc gctggcagag tttctgcaaa gtggtgctgc cggaactttc
    65701 tccggcgctg gtggcgggcg tggcgctgtc gtttacccgt agtcttggtg aatttggcgc
    65761 ggtgattttt atcgccggaa atatcgcgtg gaagacggaa gtgacgtcgc tgatgatttt
    65821 tgtgcgctta caggagtttg attacccggc agcgagcgcg attgcttcgg tgatcctcgc
    65881 ggcatctctg ctgctgctgt tctcaattaa cactctgcaa agtcgctttg gtcggcgtgt
    65941 ggtaggtcat taatggcgga agttacccaa ttgaagcgtt atgacgcgcg cccgattaac
    66001 tggggcaaat ggtttctgat tggcatcggg atgctggtat cggcgttcat cctgctggtg
    66061 ccgatgattt atatcttcgt acaggcgttc agcaaagggc tgatgccggt tttacagaat
    66121 ctggccgatc cggacatgct gcacgccatc tggctgacgg tgatgatcgc gctgattgcc
    66181 gtaccggtaa acctggtgtt cggcattctg ctggcctggc tggtgacgcg ctttaacttc
    66241 cctggacgcc agttactgct gacgctactg gacattccgt ttgccgtatc gccggtggtt
    66301 gccggtctgg tgtatctgct gttctacggc tctaacggcc cgctgggcgg ctggctggat
    66361 gaacataatc tgcaaattat gttctcctgg cccggaatgg tgctggtcac catctttgtg
    66421 acgtgtccgt ttgtggtgcg cgagctggtg ccggtgatgt taagccaggg cagccaggaa
    66481 gacgaagcgg cgattttgct tggcgcgtct ggctggcaga tgtttcgccg cgtcacattg
    66541 ccgaacattc gctgggcgct gctttatggc gtggtgttga ccaacgcccg cgcaattggc
    66601 gagtttggcg cggtgtcggt ggtttccggc tcgattcgcg gcgaaaccct gtcgctgccg
    66661 ttacagattg aattgctgga gcaggactac aacaccgtcg gctcctttac cgctgcggca
    66721 ctgttaacgc tgatggcgat tatcaccctg tttttaaaaa gtatgttgca gtggcgcctg
    66781 gagaatcagg aaaaacgcgc acagcaggag gaacatcatg agcattgaga ttgccaatat
    66841 taagaagtcg tttggtcgca cccaggtgct gaacgatatc tcactggata ttccttcagg
    66901 tcagatggtc gcgttgctgg ggccgtccgg ttccgggaaa accacgctgc tgcgcattat
    66961 cgccgggctg gagcatcaaa ccagcgggca tattcgcttc cacggcaccg acgtcagccg
    67021 cctgcacgcg cgcgatcgca aggtcggttt cgtgttccag cattacgcgc tgttccgcca
    67081 tatgacggta ttcgacaata tcgcttttgg cctgacggtg ctgccgcgtc gcgatcgccc
    67141 gaatgccgca gccatcaaag cgaaagtgac aaaattgctg gagatggtgc agcttgctca
    67201 tctggcggat cgttatccgg cacagctttc cggcggccag aaacagcgcg tggcgctggc
    67261 gcgcgcgctg gctgtagaac cgcaaattct gctgcttgat gaaccgtttg gcgcgctgga
    67321 tgcgcaggtg cgtaaagagc tgcgtcgctg gctgcgtcaa ctgcatgaag agctgaaatt
    67381 caccagcgtg ttcgtgaccc acgaccagga agaagcgacg gaagtagctg atcgtgtagt
    67441 ggtgatgagc cagggcaata ttgaacaggc tgatgcgccg gagcaggtat ggcgcgaacc
    67501 agcgacccgt tttgtgctcg aatttatggg cgaagtgaac cgcctgcagg gaaccattcg
    67561 cggcgggcag ttccatgttg gcgcacatcg ctggccgctg ggctacacac ctgcttatca
    67621 ggggccggtg gatctcttcc tgcgcccgtg ggaagtggat atcagccgcc gtaccagtct
    67681 cgattcgccg ctgccggtac aggtactgga agccagcccg aaaggtcact acacccaatt
    67741 agtggtgcag ccgctggggt ggtacaacga accgctgacg gtcgtgatgc atggcgacga
    67801 tgccccgcag cgtggcgagc gtttattcgt tggtctgcaa catgcgcggc tgtataacgg
    67861 cgacgagcgt atcgaaaccc gcgatgagga acttgctctc gcacaaagcg cctgataggt
    67921 tgagtgaatg ttaaacgccc ggaggcgctt cccgcgatcc gggcttttta atggcaaggt
    67981 ttgtaacctg tagacctgat aagacgcgca agcgtcgcat caggcaacac cacgtatgga
    68041 tagagatcgt gagtacatta gaacaaacaa taggcaatac gcctctggtg aagttgcagc
    68101 gaatggggcc ggataacggc agtgaagtgt ggttaaaact ggaaggcaat aacccggcag
    68161 gttcggtgaa agatcgtgcg gcactttcga tgatcgtcga ggcggaaaag cgcggggaaa
    68221 ttaaaccggg tgatgtctta atcgaagcca ccagtggtaa caccggcatt gcgctggcaa
    68281 tgattgccgc gctgaaaggc tatcgcatga aattgctgat gcccgacaac atgagccagg
    68341 aacgccgtgc ggcgatgcgt gcttatggtg cggaactgat tcttgtcacc aaagagcagg
    68401 gcatggaagg tgcgcgcgat ctggcgctgg agatggcgaa tcgtggcgaa ggaaagctgc
    68461 tcgatcagtt caataatccc gataaccctt acgcccatta caccactacc gggccggaaa
    68521 tctggcagca aaccggcggg cgcattaccc attttgtctc cagcatggga acaaccggca
    68581 ctatcaccgg cgtttcacgc tttatgcgcg aacaatccaa accagtaacg attgttggcc
    68641 tgcagccgga agaaggcagc agtattcccg gcattcgccg ctggcctgcg gaatatctgc
    68701 cggggatttt caacgcttct ctggtggatg aggtgctgga tattcatcag cgcgatgcag
    68761 aaaacaccat gcgcgaactg gcagtgcggg aaggaatatt ctgtggcgtc agctccggcg
    68821 gcgcggttgc cggagcactg cgggtggcaa aagctaaccc tggtgcggtg gtggtagcga
    68881 tcatctgcga tcgtggcgat cgctaccttt ccaccggggt gtttggggaa gagcatttta
    68941 gtcagggggc ggggatttaa gattattgcg caggcaccgt tttatccgtc tgcgcaatta
    69001 aggcatgtaa aaaaggctgt gcgttctcat cgctcttacc ggggatcttc acaatatacg
    69061 gcttcccggt aatcgatgac gacgaagcaa ccttatcaat aaactgctcg gcagtgtcaa
    69121 tgcggttacg ggtattgccg agcttcagac gcagatgaga aaccgcttca tcgcaggtat
    69181 gttcatcacc gttgcgcaca aatatcaaat cctttttctg tgctaatccc tccagcatgg
    69241 cgttgatgcg ggcttcttca tgaacagtta acttcgcgta agcggggagt gtcattagta
    69301 gtgtaatgac cagacaaatg attttcttca ttgaattacc ttatccttgt tttgtgatag
    69361 ccgatttgac tgccagaatc aggtaaagtt tcggttggaa tacgatgagg atcatcggca
    69421 aggagaaaaa attaaatgag tagtttgttg ttgtttaacg ataagagtag ggcgctgcag
    69481 gcggatatcg tcgccgtgca gtcgcaggta gtgtacggca gcgtaggcaa cagcattgcg
    69541 gtgcctgcta taaaacagaa cggcttgaat gtctttgccg tgccgacggt attgctgagc
    69601 aatacgccgc attatgacac tttctacggt ggtgcgattc cggacgaatg gtttagcggc
    69661 tatttgcgtg cgcttcagga gcgtgatgcg ctgcgccaac ttcgtgctgt aaccacgggc
    69721 tatatgggaa cggcatcgca aatcaaaatc cttgccgagt ggctgactgc gctacgcaaa
    69781 gaccatcctg acctattgat catggtcgat ccggtgattg gcgatattga tagcggaatt
    69841 tatgtcaaac ctgaccttcc cgaggcgtat cgacaatatt tactgccgct ggcgcaagga
    69901 attaccccca atatctttga gctggaaatc ctgaccggta aaaattgccg cgatctcgac
    69961 agtgccattg ctgccgcaaa aagtctgctt tcagacacat taaaatgggt ggtgattacc
    70021 agcgcctccg gtaatgaaga aaatcaggag atgcaggtgg tggtggtcag tgccgacagc
    70081 gtgaatgtca tttcccattc acgggtaaaa accgacctga aagggactgg cgacctgttt
    70141 tgtgctcagc ttatcagtgg tttgctgaaa gggaaggcgt taaacgatgc ggtgcaccgt
    70201 gcggggttgc gcgtactgga agtgatgcgc tacacccagc agcatgagag cgatgaattg
    70261 attttgccgc cgctggcgga agcataaaaa atggcgccga tgg
//
LOCUS       DAESHI010000006        67996 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.006, whole genome shotgun sequence.
ACCESSION   DAESHI010000006 DAESHI010000000
VERSION     DAESHI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67996
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.006"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            1..424
                     /locus_tag="KW011_000402"
                     /pseudo
     CDS             1..424
                     /locus_tag="KW011_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase"
     gene            813..1443
                     /locus_tag="KW011_000403"
                     /pseudo
     CDS             813..1443
                     /locus_tag="KW011_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1546..2048
                     /locus_tag="KW011_000404"
                     /pseudo
     CDS             1546..2048
                     /locus_tag="KW011_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(2115..3572)
                     /gene="nuoN"
                     /locus_tag="KW011_000405"
     CDS             complement(2115..3572)
                     /gene="nuoN"
                     /locus_tag="KW011_000405"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HBH7658841.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3579..5108)
                     /gene="nuoM"
                     /locus_tag="KW011_000406"
     CDS             complement(3579..5108)
                     /gene="nuoM"
                     /locus_tag="KW011_000406"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HBH7658842.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(5272..7113)
                     /gene="nuoL"
                     /locus_tag="KW011_000407"
     CDS             complement(5272..7113)
                     /gene="nuoL"
                     /locus_tag="KW011_000407"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HBH7658843.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(7110..7412)
                     /gene="nuoK"
                     /locus_tag="KW011_000408"
     CDS             complement(7110..7412)
                     /gene="nuoK"
                     /locus_tag="KW011_000408"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HBH7658844.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7409..7963)
                     /gene="nuoJ"
                     /locus_tag="KW011_000409"
     CDS             complement(7409..7963)
                     /gene="nuoJ"
                     /locus_tag="KW011_000409"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HBH7658845.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7975..8517)
                     /gene="nuoI"
                     /locus_tag="KW011_000410"
     CDS             complement(7975..8517)
                     /gene="nuoI"
                     /locus_tag="KW011_000410"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HBH7658846.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8532..9509)
                     /gene="nuoH"
                     /locus_tag="KW011_000411"
     CDS             complement(8532..9509)
                     /gene="nuoH"
                     /locus_tag="KW011_000411"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HBH7658847.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9506..12232)
                     /gene="nuoG"
                     /locus_tag="KW011_000412"
     CDS             complement(9506..12232)
                     /gene="nuoG"
                     /locus_tag="KW011_000412"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HBH7658848.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(12285..13622)
                     /gene="nuoF"
                     /locus_tag="KW011_000413"
     CDS             complement(12285..13622)
                     /gene="nuoF"
                     /locus_tag="KW011_000413"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBH7658849.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13619..14119)
                     /gene="nuoE"
                     /locus_tag="KW011_000414"
     CDS             complement(13619..14119)
                     /gene="nuoE"
                     /locus_tag="KW011_000414"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HBH7658850.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(14122..15924)
                     /gene="nuoC"
                     /locus_tag="KW011_000415"
     CDS             complement(14122..15924)
                     /gene="nuoC"
                     /locus_tag="KW011_000415"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HBH7658851.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(16018..16680)
                     /gene="nuoB"
                     /locus_tag="KW011_000416"
     CDS             complement(16018..16680)
                     /gene="nuoB"
                     /locus_tag="KW011_000416"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HBH7658852.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16696..17139)
                     /gene="nuoA"
                     /locus_tag="KW011_000417"
     CDS             complement(16696..17139)
                     /gene="nuoA"
                     /locus_tag="KW011_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HBH7658853.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17768..18706)
                     /gene="lrhA"
                     /locus_tag="KW011_000418"
     CDS             complement(17768..18706)
                     /gene="lrhA"
                     /locus_tag="KW011_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HBH7658854.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19626..20843
                     /gene="alaA"
                     /locus_tag="KW011_000419"
     CDS             19626..20843
                     /gene="alaA"
                     /locus_tag="KW011_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HBH7658855.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20927..21526
                     /gene="yfbR"
                     /locus_tag="KW011_000420"
     CDS             20927..21526
                     /gene="yfbR"
                     /locus_tag="KW011_000420"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HBH7658856.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21585..23417)
                     /locus_tag="KW011_000421"
     CDS             complement(21585..23417)
                     /locus_tag="KW011_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBH7658857.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23504..24152)
                     /gene="hxpA"
                     /locus_tag="KW011_000422"
                     /pseudo
     CDS             complement(23504..24152)
                     /gene="hxpA"
                     /locus_tag="KW011_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(24163..24657)
                     /locus_tag="KW011_000423"
     CDS             complement(24163..24657)
                     /locus_tag="KW011_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HBH7658858.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24740..25195)
                     /locus_tag="KW011_000424"
     CDS             complement(24740..25195)
                     /locus_tag="KW011_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HBH7658859.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25533..26735
                     /gene="ackA"
                     /locus_tag="KW011_000425"
     CDS             25533..26735
                     /gene="ackA"
                     /locus_tag="KW011_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBH7658860.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26810..28954
                     /gene="pta"
                     /locus_tag="KW011_000426"
     CDS             26810..28954
                     /gene="pta"
                     /locus_tag="KW011_000426"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBH7658861.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            29144..30664
                     /gene="yfcC"
                     /locus_tag="KW011_000427"
     CDS             29144..30664
                     /gene="yfcC"
                     /locus_tag="KW011_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBH7658862.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30697..31239)
                     /gene="yfcD"
                     /locus_tag="KW011_000428"
     CDS             complement(30697..31239)
                     /gene="yfcD"
                     /locus_tag="KW011_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HBH7658863.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(31297..31848)
                     /gene="yfcE"
                     /locus_tag="KW011_000429"
     CDS             complement(31297..31848)
                     /gene="yfcE"
                     /locus_tag="KW011_000429"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBH7658864.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31904..32548)
                     /gene="yfcF"
                     /locus_tag="KW011_000430"
     CDS             complement(31904..32548)
                     /gene="yfcF"
                     /locus_tag="KW011_000430"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HBH7658865.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32684..33331
                     /gene="yfcG"
                     /locus_tag="KW011_000431"
     CDS             32684..33331
                     /gene="yfcG"
                     /locus_tag="KW011_000431"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HBH7658866.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33388..33750
                     /gene="folX"
                     /locus_tag="KW011_000432"
     CDS             33388..33750
                     /gene="folX"
                     /locus_tag="KW011_000432"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HBH7658867.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33771..34664
                     /locus_tag="KW011_000433"
     CDS             33771..34664
                     /locus_tag="KW011_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HBH7658868.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34712..35602)
                     /gene="rpnB"
                     /locus_tag="KW011_000434"
     CDS             complement(34712..35602)
                     /gene="rpnB"
                     /locus_tag="KW011_000434"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HBH7658869.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLESNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35799..36572)
                     /gene="hisP"
                     /locus_tag="KW011_000435"
     CDS             complement(35799..36572)
                     /gene="hisP"
                     /locus_tag="KW011_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HBH7658870.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36580..37296)
                     /locus_tag="KW011_000436"
     CDS             complement(36580..37296)
                     /locus_tag="KW011_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH7658871.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(37293..37979)
                     /gene="hisQ"
                     /locus_tag="KW011_000437"
     CDS             complement(37293..37979)
                     /gene="hisQ"
                     /locus_tag="KW011_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HBH7658872.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(38069..38851)
                     /gene="hisJ"
                     /locus_tag="KW011_000438"
     CDS             complement(38069..38851)
                     /gene="hisJ"
                     /locus_tag="KW011_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HBH7658873.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(39072..39854)
                     /gene="argT"
                     /locus_tag="KW011_000439"
     CDS             complement(39072..39854)
                     /gene="argT"
                     /locus_tag="KW011_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HBH7658874.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLCKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(40120..40689)
                     /gene="ubiX"
                     /locus_tag="KW011_000440"
     CDS             complement(40120..40689)
                     /gene="ubiX"
                     /locus_tag="KW011_000440"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HBH7658875.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40784..42301)
                     /gene="purF"
                     /locus_tag="KW011_000441"
     CDS             complement(40784..42301)
                     /gene="purF"
                     /locus_tag="KW011_000441"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBH7658876.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(42338..42826)
                     /gene="cvpA"
                     /locus_tag="KW011_000442"
     CDS             complement(42338..42826)
                     /gene="cvpA"
                     /locus_tag="KW011_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HBH7658877.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(43085..43747)
                     /gene="dedD"
                     /locus_tag="KW011_000443"
     CDS             complement(43085..43747)
                     /gene="dedD"
                     /locus_tag="KW011_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HBH7658878.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43737..45005)
                     /gene="folC"
                     /locus_tag="KW011_000444"
     CDS             complement(43737..45005)
                     /gene="folC"
                     /locus_tag="KW011_000444"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBH7658879.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(45075..45989)
                     /gene="accD"
                     /locus_tag="KW011_000445"
     CDS             complement(45075..45989)
                     /gene="accD"
                     /locus_tag="KW011_000445"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HBH7658880.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(46145..46804)
                     /locus_tag="KW011_000446"
     CDS             complement(46145..46804)
                     /locus_tag="KW011_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBH7658881.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(46887..47699)
                     /gene="truA"
                     /locus_tag="KW011_000447"
     CDS             complement(46887..47699)
                     /gene="truA"
                     /locus_tag="KW011_000447"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBH7658882.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47699..48712)
                     /locus_tag="KW011_000448"
     CDS             complement(47699..48712)
                     /locus_tag="KW011_000448"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBH7658883.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48778..49914)
                     /gene="pdxB"
                     /locus_tag="KW011_000449"
     CDS             complement(48778..49914)
                     /gene="pdxB"
                     /locus_tag="KW011_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HBH7658884.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            50013..51008
                     /gene="flk"
                     /locus_tag="KW011_000450"
     CDS             50013..51008
                     /gene="flk"
                     /locus_tag="KW011_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HBH7658885.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(51005..52183)
                     /locus_tag="KW011_000451"
     CDS             complement(51005..52183)
                     /locus_tag="KW011_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH7658886.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52458..53678)
                     /gene="fabB"
                     /locus_tag="KW011_000452"
     CDS             complement(52458..53678)
                     /gene="fabB"
                     /locus_tag="KW011_000452"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HBH7658887.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            53837..55843
                     /gene="mnmC"
                     /locus_tag="KW011_000453"
     CDS             53837..55843
                     /gene="mnmC"
                     /locus_tag="KW011_000453"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HBH7658888.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55964..56242)
                     /locus_tag="KW011_000454"
     CDS             complement(55964..56242)
                     /locus_tag="KW011_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HBH7658889.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(56276..56824)
                     /gene="epmC"
                     /locus_tag="KW011_000455"
     CDS             complement(56276..56824)
                     /gene="epmC"
                     /locus_tag="KW011_000455"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HBH7658890.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56824..57633)
                     /locus_tag="KW011_000456"
     CDS             complement(56824..57633)
                     /locus_tag="KW011_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBH7658891.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57633..58457)
                     /gene="mepA"
                     /locus_tag="KW011_000457"
     CDS             complement(57633..58457)
                     /gene="mepA"
                     /locus_tag="KW011_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HBH7658892.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58461..59546)
                     /gene="aroC"
                     /locus_tag="KW011_000458"
     CDS             complement(58461..59546)
                     /gene="aroC"
                     /locus_tag="KW011_000458"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBH7658893.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59581..60513)
                     /gene="prmB"
                     /locus_tag="KW011_000459"
     CDS             complement(59581..60513)
                     /gene="prmB"
                     /locus_tag="KW011_000459"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBH7658894.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            60679..61230
                     /gene="smrB"
                     /locus_tag="KW011_000460"
     CDS             60679..61230
                     /gene="smrB"
                     /locus_tag="KW011_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HBH7658895.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(61352..62224)
                     /locus_tag="KW011_000461"
     CDS             complement(61352..62224)
                     /locus_tag="KW011_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HBH7658896.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(62211..62735)
                     /locus_tag="KW011_000462"
     CDS             complement(62211..62735)
                     /locus_tag="KW011_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBH7658897.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62732..63202)
                     /locus_tag="KW011_000463"
     CDS             complement(62732..63202)
                     /locus_tag="KW011_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBH7658898.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            complement(63199..63747)
                     /locus_tag="KW011_000464"
     CDS             complement(63199..63747)
                     /locus_tag="KW011_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBH7658899.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63722..64473)
                     /locus_tag="KW011_000465"
                     /pseudo
     CDS             complement(63722..64473)
                     /locus_tag="KW011_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64493..67135)
                     /gene="yfcU"
                     /locus_tag="KW011_000466"
                     /pseudo
     CDS             complement(64493..67135)
                     /gene="yfcU"
                     /locus_tag="KW011_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(67217..67780)
                     /locus_tag="KW011_000467"
     CDS             complement(67217..67780)
                     /locus_tag="KW011_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBH7658900.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        17020 a        18345 c        16869 g        15762 t
ORIGIN      
        1 tgataaagaa aattcactta agcgtgtcat taatagtttt aattctgaac tcatgggata
       61 aatgtcgaat aataatataa aagttcattt aaatgaacca gagataatat ttttacatgc
      121 cgttcttcag caatacttaa gccaaagttg cggtgcgttt gtgtgcatgg cagcccagga
      181 agtgattgaa caaagggaaa gcaattctga cagcgccccc tatacgttat taaaaaacta
      241 tgctgacaga tttaaaaaat attcagcaga agagcagtac gaaattgatt tttaacatcg
      301 actggtaaac aggaattgtt atttagataa atatggcgat gcaaatatca atgattatta
      361 tagagactta gaaataaaac actcacaacg caaaaataga gcatcaggaa aaagagtaag
      421 ttaacatcat tgttaattaa ttcgaccagt cagcaaatat gctgactggt cgaacatagt
      481 ttgctaaaca gtcatcactg actgatgtca gtcacaccat ccgccagttc aatcttcggg
      541 tatttccagc gtatctcggc acgcgattgc tggtctgttg atgtagtgga ttgttgttgc
      601 tgacttttct taccctcaga gttctgcccc ctgtcaactc aatctgccaa taaataacat
      661 aattatattt ttccttatat gaattccttt ttaaaatagc gaagccactg taaaagatac
      721 tttttgtata gatctaacaa ccgtaataaa acaaacactc ataactcatt ataaataatg
      781 ataaaataac gtcttttatt gataatttat atatggaaaa aaattccttt tgttatattg
      841 tttctgattt cactccctct gattattttt accaaccctg ggtcactgca ttatcgccaa
      901 cgccccgaca cgtcagtcct gagcaattag aaaaaacagt tcgttatctt acacaaactg
      961 tgcatccacg taatgccgac aatattgata atctgaatag gtccgtagag tatataaaag
     1021 agttctttgt cagtagcggt gccagagtta ccgcgcagag cgtccccgat tggctattac
     1081 gactctgcgc ctggtagcca ggattaccct tacccgcaat gtcctggctt tatcccgatc
     1141 ggggggactt tattgcctgg tcggcagaat acaggatatc aacgccgttc gtcaggtaaa
     1201 aacggcattg ttatcatccc gggatttatc tgtttattct atgaatgccc catggtttat
     1261 tcccggtatt gatttctctg accacctgaa ttattggcaa cacgatattc ccgccgtaat
     1321 gattactgac accgcttttt atcgtaataa acaataccac ttgcccggtg atacagcaga
     1381 cagattgaat tatcagaaaa tggctcagat ggtgttataa ccttgttaca caacagtaaa
     1441 taataaaaat ttctcgaggc gggtccagaa acagcgacat gtacatttta ttttccctcg
     1501 tctgttttaa gatgcgaaac ataaaattca atggaggaaa aataaatggg aatgattggt
     1561 tattttgcag aaattgactc agaaaagatt aaccaactgc ttgagtctac tgaaaaacct
     1621 ttgatggata atatacatga cacgctttca ggattacgcc gactggatat tgataaacgc
     1681 tgggattttt tacactttgg gctgacaggc acccctgctt ttgatcccgc gaagaatgat
     1741 ccgctgagtc gtgccgtgct tggtgaacac agcctcgaag atggcataga tggcttcctg
     1801 ggattgacct ggaatcagga gttagccgcg acaatcgacc gactggaatc actggatcga
     1861 agtgagctgc gtaaacagtt ttccattaag cgactcaatg aaatggagat ttatccggga
     1921 gtgacctttt cagaagagtt agaggggcaa ctctttgctt cgatcatgct gatatggaaa
     1981 agctgattag cgcgtatcga cgtatgctgc gacaagggaa tcatgcgctg acggttattg
     2041 ttgggtgatg gtgtcagggc accaaaaaaa accgccgaac atgtcggcgg ctttctgact
     2101 tacaaactga tagcttacat cagcggcatt gctaaccgca caatgctaat cagcggttgt
     2161 ggccatacac ccagcaccag taccaacagt gcagagatca gcaccacgat accgcctgcg
     2221 ctgtactgcc agtttgacgg tgcatcgcga cccggttgtt ccggggcgtg caggtacagg
     2281 ctcaccgcca cgcgcaggta gtagtaaagg ccgcttgccg aaccgacaac cacggcgccc
     2341 accagccacc acaagtgtgc ctggacaccg accgccagca cgtagaactt accaataaag
     2401 cccagcgtca tcggaatacc ggccagagac agcatcatca ccgtcatcac cgccgcgagg
     2461 atcgggcgat gccagaacag tccgcggtaa gagtacaggg aatcagcatc cgggccacga
     2521 tacgggctgg acatcaggct gaccacgccg aacgcgccga ggctgctaaa cagataaccg
     2581 gccaggtaaa cccctaccgc ttccatcgac atctcgccag tttgcagcgc aatcagcgcc
     2641 accagcagat agccgaggtg agagatagat gagtaaccga gcagacgttt gatattggtc
     2701 tggctcagcg ccatcaggtt accgaagatg atggaggcaa aggcgataat cgccagcacc
     2761 acgcgaatcg cttcgctgtc acccatcggt gcgtagagga acagacgcat caccacaccg
     2821 aagatagcga ttttgctcgc cgtcgccagg aaagtggaaa ccggcgcagg cgcgccctgg
     2881 tatacgtctg gcgtccacag gtggaacggc accagagaga gtttgaagcc gaggccaaca
     2941 atcatcaggc cgaaacctgc cagcaacagc ggctcgttga gcataccgtc gccaaggttt
     3001 ttacccaacg cgacaaacga caggtcgcca gactgcgcat acaccagcgc cataccaaac
     3061 agcaggaaag aagacgctgc ggcagaaagg atggtgtatt tgatactggc ttccagtgaa
     3121 cgtttctggc ggaaagcgta accgaccagg ccaaacagcg gcaaagagat cagttcgata
     3181 ccgaggaaca gagacgccag atggttggca ttcgccagca ggatcccgcc cagcgcggca
     3241 attaacacca acaggtagaa ctcgtccttg ttgtcgttat agccttcaag ccacgggtag
     3301 gcgaaagtac aggtggcgag gctcgccaac aataccagcc cggtgtaaag catggcgaaa
     3361 ccatcaacgc gcatcagcgg cgtaacgtcc atagcgcctg cctggccgac aaaccagagc
     3421 gaaaccagcg ccgcgttaag cccaataacc gagagcgtag cgttgaggaa atgattgcgt
     3481 cgccacgcaa tggagagcat cacaaccacc accgtcaagc cgacgatcag caacggtagc
     3541 agtgcgatca ggttttgtgg agttattgtc atggcgattt acggccttgt agtagtaacg
     3601 gaattaacaa accactgctg gatattgcca atcgcggagt gcgaggtatc cagaatcggc
     3661 tgcggataga agccctgcag taccagcagc accaccagca acaggatcat aaacagctca
     3721 cgcagcgaca tccctggcag ttcctggctg gcaatctcgc ttttcgcttt accgaagtaa
     3781 gcgcgatgca acatcgccag cgaataaaca gatgcaaaga ccagcccaaa ggtagagata
     3841 acggtaatca ccgggacaac ctggaagctg ccgaacagaa tcataaattc gccgacgaag
     3901 ttaccggtgc caggcatccc aagcgttgcc accgcaaaga acagcgacag tgctggcagc
     3961 catttcatct tgctccacag accgcccatc atgcgcatgt cgcgggtatg gatacgttca
     4021 taaagctgac cacaaagaat aaacagaccc gccgccgaca agccgtgcgc aatcatctgg
     4081 attaccgcgc cctggtaggc caactggctg ccggtgtaga tagcaatcag cacgaagccc
     4141 atgtgggaaa ccgaggtgta ggcgatcaga cgtttgatat cggtctgggc gaaggccatc
     4201 cacgcaccgt agaagatgcc gataacaccc agccacatag caattggcgc gaactctgcc
     4261 gacgcgttcg ggaacagcgg cagggagaaa cgcagcaaac cgtaagcggc agttttcagc
     4321 aagatccccg cgaggtcaac ggaaccggcg gtcggagcct gggagtgcgc atccggcagc
     4381 cagccatgca gcggaaccac cggcattttg actgcgaagg cgatgaagaa gcccagcatt
     4441 aacaggtatt ccgcaccact ggacattggc gtattcagca gctcttcata gttgaaggtc
     4501 cagacgccgg tcgcattgta gtgaacaaaa accagcgcca ggatggcaat caacatcacc
     4561 agaccactcg cctgggtgta aatgaagaac ttggttgccg ccgtgatacg cgttttaccg
     4621 tcagaggctt tatgccccca cagtgcgatc aggaagtaca tcggcaccag catcatttcc
     4681 cagaagaaga agaacaggaa catgtcgatg gcaaggaaca cgccgataac gccgcccagg
     4741 atccacatca ggttgaggtg gaagaagccc tgatattttt cgatctcttt ccacgaacat
     4801 agtaccgcca gcacaccgag cagaccggtc agcacgacca tcagcagcga cagcccgtca
     4861 atggcgagat gaatagagat acccaaacgc gggatccacg gcatgtcgaa ttcagactgc
     4921 cactgcggaa ttccggcgga ttgcgtcagt gaataaccgc cctgcaacca cagttgcagc
     4981 gacagcgcca gcgtcaatcc catggtgatc agcgcgatcc agcgtggcac cttgacgcca
     5041 aagcgttcgg tctgccagca caggaagccg ccaataaagg gaattaatat tagccagggt
     5101 agtaacatgg cgatctttat tccttgtaaa agtcccgtca ggaccggatt ttcaacgaat
     5161 tctcacgaca gaaaattctc ttcggactgg gggtagtgtc ggatgcaccg cgaaggcggc
     5221 ttatccgacc tacgttctta tatgcccagg ggcaatccca caatccttaa ctcaacgcag
     5281 taccatcaac agtgccagca ccacgaccgc accgatgctc atggatgcca cataccagcg
     5341 cagatagccg ttctcactta acagcagacc tttacctgca aagcgggaaa ggacagccgg
     5401 aatgttcatc attgagttca gcggatcgcg tttcagcaac caggcaatac ccaggaacgg
     5461 cctgacgaac actttgtcgt acagccagtc aaagccccag gcgttgtacc accaggtact
     5521 cagcaggcgg cccggcgcac tgttggcgat ggaggtcacc agagtacgtt tacccagcca
     5581 cagccaagct gccagcagaa tgccgacaat cgcgaccacg ccagaggtaa tttccagggt
     5641 caacatgctg ccgtgcgcca gttccgtcgt ttgcggaagc acgccctgca gcggcggtac
     5701 aatcagtgcg ccaacgaagg tggaaaggat cagcagcaca atcagcggca ggctgtgagt
     5761 tacccctttc acggcgtgag cgtgaatttg ttcttttccg tggaagacga tgaaaatcat
     5821 acggaaggtg tagagcgagg tcataaacgc accgaccaga cctgccacca tcagattgat
     5881 atgaccattc gccatcgcac ccgcgaggat ctcatcctta ctgaagaagc ccgcagtgac
     5941 cagcggcagt gccgacagtg ctgcgccgcc caccaggaag cagagataaa ccagcggaat
     6001 agatttacgc agaccgccca tcttgaagat gttctgttcg tgatggcagg ccagaatgac
     6061 ggaaccggat gccaggaaca gcagcgcttt aaagaacgcg tgagtcatca agtggaaaat
     6121 cgccgcatcc catgcctgca cgccaagcgc gaggaacatg tagccaatct ggctcatggt
     6181 agagtaagcg agaacacgtt tgatgtcggt ctgtaccagc gcggcaaaac cggccagcag
     6241 cagcgtaacc gccccgacaa tgcccaccag atgcagaatt tccggcgtca tcaggaacag
     6301 gccgtgggta cgggcgatca ggtagacacc cgcggttacc atggttgcgg cgtggatcag
     6361 cgcggagaca ggcgtcgggc ccgccatcgc gtcggcaagc catgtctgca acggcaactg
     6421 cgcagattta ccgaccgcac cgcccagcag catcagcgtc gcccacatca gcatgttatt
     6481 gccgtcagca aagtgcgctg gtgccagttc caccatttcg cggaagttca gggtgcccag
     6541 ttcgttgtaa agaatgaaca gtgcgaaagc gaggaaaacg tcacccacac gggtcacgac
     6601 gaacgctttc attgccgctg cgccattctt cggatcggtg taatagaaac cgatcagcag
     6661 ataggagcac aggcccacgc cttcccagcc gaggtacatc agcagcaggt tgtcggcaag
     6721 caccagaact accatgctgg cgatgaacag gttggtgtag gcgaagaagc gagagtagcc
     6781 ctcttcaccg cgcatgtacc aggaggcgta catgtgaata aggaaaccca caccagtgac
     6841 caccgagagc atggtcagcg acaggccgtc cagcaccagg ttaaaaccga tgttaaagtc
     6901 gcctaccgac atccacgtcc acagcggctg gctgtatgcc tgctcgccgt tagcgaagaa
     6961 atcaacgccg ataaaggcgg ttaccagcgc cgccaggccc acagagccaa cgccgacgat
     7021 cgccgaaacg ttttcagacc agcgcccacg ggagaacgcc agcaggacga agccaatcaa
     7081 tggcaaaata atggttaagg caagcatgtt catccgcgca tctcacttac tgaatcgatg
     7141 ttcaggttct ggcgacgacg gtgaagttgc agcagcagcg caaggccgat actcgcttct
     7201 gccgccgcga ggctgatggc gagaatgtac atcacctgac cgtcggtctg accccagtag
     7261 cttccggcga caacaaaggc cagcgcggag gcgttaatca tgatttccag accaatcagc
     7321 ataaacagca gattgcgacg gataaccaga ccggttaagc caagaatgaa taagattgcc
     7381 gcgaggatca gtccatgttg taaggggatc atgcgtgctc ctccgttttt cttttcgcgc
     7441 tgtcgtcttt acgattgctc agcacttcac ccgcacgctc ttcacgaccg acgtggaagg
     7501 ccacaaccag acctgcgagc agcagcatag aagccagttc caccgccagt acgtaaggcc
     7561 cgaacagcgt aatacccact gctttagcac tgattggcgt accgtcgata ccctgatcgt
     7621 taacaccgag gatggcgtaa acaatcacca ccagcatgat ggccgacaaa attgccggac
     7681 caatccacac ctgcggtttc agccactggc gttcctgttc gatttctgaa ccgcccaggt
     7741 tgagcatcat caccacgaac acgaacagca ccataatggc acccgcgtag acgataattt
     7801 ccagcgcacc ggcgaagtaa gcgcccagtg agaagaacac cccggaaatc gccagcagcg
     7861 aaataatcag gtacagcagt gcgtgtaccg gattggtatg ggtgatcact cgcaaggtcg
     7921 caagtatggc tatcaggcca cagatataaa aagcgaactc cattgcccct ctccttacgg
     7981 taacaggctc ttgacgtcga taggcttggc ttcgttctct gcttcgccct tatctttgcc
     8041 gtcgattgcc atacctgcca tccggtagaa gttatattcc gggtatttgc ccggaccgga
     8101 gatcagcaga tcctcttttt cgtaaaccag atcctggcgc ttgtattccc ccatttcgaa
     8161 atccggggtt aactgaatcg ccgtggtcgg acaggcttct tcgcacagac cacagaaaat
     8221 gcagcgtgag aagttgatgc ggaaaaattc cggataccag cgaccgtctt tggtttctgc
     8281 tttttgcagc gagatacagc cgaccgggca ggctaccgcg cagaggttac aggctacgca
     8341 acgctcttcg ccgtccgggt cgcgggtcag aacgatacga ccacgataac ggggcggcag
     8401 atagatcggc tcttccgggt acattcgcgt ttcgcgtttg gcgaacgcgt gcaggccgat
     8461 catccagata ctacgaacct gggtgccgaa acctactaac aattctttta aggtcatggt
     8521 cttattgccc cttattgcgc ctgccagaga atgacagccg ccgttaccag caagttgatc
     8581 agcgtcagcg gcaggcagat tttccagccg aaggacatta cctggtcata acgcggacgc
     8641 ggtaacgacg cacgaatcaa aatgaacatc atcataaaga acgcggtttt cagcgcgaac
     8701 cagatgaatg gcggtaacaa cgggccttgc cagccaccga agaacagcgt caccatcaat
     8761 gcagagatgg tcacaatccc gatgtactca cccacgaaga acagaccgaa cttcataccg
     8821 gaatattcaa tgtggtaacc atccgccagt tcctgctcgg cttccggctg gtcaaacggg
     8881 tgacggtgac ataccgccac gcccgcgatg gcaaaggtaa taaaaccaaa gaattgcggg
     8941 ataacgttcc acacatgcgc ctggctgttg acgatgtcgg tcatgttgaa tgaaccggcc
     9001 tgcgccacca cgcccatcag ggaaagcccg aggaacactt cgtagctcag ggtctgcgca
     9061 gaagcacgca tcgcacccag caacgagtat ttgttgttac ttgaccagcc cgcaaacagc
     9121 accgcgtaaa ccgccagacc tgccatcatc aggaagaaca aaatcccgat gttcaggtcg
     9181 gcaaccaccc aacccggact gactggcaca atcgcaaagg ccagcagcag cgaggtaaag
     9241 gcaatcatcg gtgccagggt aaagatgacg cgatccgaga atttcgggat ccagtcttct
     9301 ttaaagaaca ttttgatcat gtccgcaacc agctggagcg aaccgcccca gccaacacgg
     9361 ttaggtccgt aacggttctg gaacagaccc agcaggcgac gttcgccaaa gctcatgaat
     9421 gccccgcagg tgacaaccac cagcaggatc accaccgctt tgaggatggt cagcaggatc
     9481 tcaatcagtt ccggtgatat ccaactcatt gttgtgcctc cttgagatcc tcaagatgcg
     9541 cgccagccag caccggagca ataccggaca tacccatcgg caagcccacc tgccctgccg
     9601 tcagcccttc agcgatttca accggcagcg tgaccgtatt gccatcgtaa ctaaaggaga
     9661 cgtgtgtacc tgcgttcaca cctaacttcg cggcatccgc tgggttgagt ttgatgtacg
     9721 gctgcggcat acggctctgg aagaccggag cacgctgtga caattcatcg ctgccaaaca
     9781 ggtggtaata cggcgcgata cgccatttcc cgtcctgcgg ctggaagcgt gccggtacgc
     9841 tggtgaagta atccagacca ttttcgctgg tttcaaacag acgcacgccc ggatcgccaa
     9901 agcgcagttt gccgcccact tcgtcctgga atttgttcca cgcctgcggg gagttccagc
     9961 ccggcgccca ggcaaacggc acttgcgaac ggtgcgcagt cggctggttg ttaccttcca
    10021 tcgagaaggt gaacatggtg tcaatatcct gcggctgacg cggctcatga acgctgatat
    10081 tggcgcgcat ggcggtacga ccgctgtaac ggtgcggttc acgggccagt ttctgcccac
    10141 gaatacggaa tgtcgcatcc ggcgcagcat ctttgatacc tgccagttcc gggattttcg
    10201 ccacaacagc gtcaatcaca tggtcgagct gcgtccagtc cacttcacgg ctcagcaggg
    10261 tgctgtgcag cgagtgtaac cagcgccagc tttccagcat gacagttttg ctgtcgtaat
    10321 aagcaggatc gtaaacctgg aagaaacgtt gggcgcggcc ttcgttgttg atcaccgtac
    10381 cgtcgctttc agcaaaactg gcagcagaaa gtaccagatg ggcgttttcc ataatcgctg
    10441 tgcgttgatg atcaaccacc atcaccagcg gcgctttagc cagcgcagca ttcacgcggg
    10501 tagcggaagc gtgacgatgc agatcgtttt ccagcaccac caccgcgtcg gcgcgtccgg
    10561 tttccagttc ggtcaacgct tcttcaagcg aaccgccacc cataatgccc agccccatgc
    10621 tgttgacgga acgggcaatc atggtgatac caacgtcagc gccgcgacct ttcagggctt
    10681 tcgcgacgtt agccgcagcc tgaatcacct ctatgctacc ggcgttcgtc ccggagataa
    10741 tcaacggttt cttcgcacct gccagtgcct gcacgatgac gtcgattttg ctttgcagct
    10801 caggttcgat accgtcaacc gctggcgcag agttatccag cgcatgggcg atggcaaaac
    10861 ctaaacgcgc ctgatcttca accggtgcgc ggtaagtcca cgccgcgata tcatccagac
    10921 gggtgtcatc aacgttggta acaaacagcg gatgcttcgc acgttgaccg atgttgagga
    10981 ttgccgcaat ctgccagtca gccactttct gtgctgccgc catttcgcgc gctttacctt
    11041 tcacagcctg acgcactgcc agcgcgacgc gcgcgccggt ctgggtaacg tcttcgccca
    11101 gcaccagtac cgcatcgtaa gattcgattt cgcgcagagc cggagtataa atgccgcctt
    11161 cacgcagcac tttcagcgcc agttgcagac gttcctgctc accgtgagcg ataccggtgt
    11221 agaagttttc ttcgcccacc agttcacgca gcgcaaagtt gctttccacg ctggcacgcg
    11281 gagaaccaat accgatcact ttcttcgact gacgcagaat atctgccgcg ccctgcattg
    11341 cctgttcggc gttgagggta atgaaatcat cgccacgacg ctgtactggc tgacgcggac
    11401 gatccttcag gttgacgtaa ccgtaaccga aacgaccacg gtcgcagagg aagtagtggt
    11461 ttaccgtacc gttgtaacgg ttttcgatac gacgcagttc gccgtaacgt tcaccggggc
    11521 tgatgttaca gccgatggaa cattgctggc agatgctcgg cgcaaactgc atatcccatt
    11581 tacggttgta acgctcggag tgcgttttgt cggtaaatac gccggtcggg caaatttcga
    11641 ccaggttacc ggaaaattcg ctttccagcg tgccgtcttc cgggcgaccg aagtagacgt
    11701 tgtcgtgcgc accgtaaacg cccagatctg taccgtcagc gtaatctttg tagtaacgca
    11761 cacagcggta gcaggcgatg cagcggttca tttcgtgaga gatgaatggc cccaaatcct
    11821 gattacggtg ggtacgtttg gtgaaacggt agcgacggaa gctgtgtccg gtcatcacag
    11881 tcatatcctg aagatggcag ttaccgcctt cttcacatac cggacagtcg tgcgggtggt
    11941 tggtcattaa ccactcgacc acactttcac ggaactgttt cgcttcttcg tcgtcaatgg
    12001 aaataaaggt gccatcggaa gccggtgtca tacaggacat caccaggcga ccacgcgtgt
    12061 cttccgcgtt ttggtattgc ttcaccgcac actggcggca agcaccgacg cttcccagcg
    12121 ccggatgcca gcaaaagtaa ggaatatcaa ggcccagaga cagacaagct tccagcaggt
    12181 tgtccgctcc gttgacctcg tattctttgc cgtctacatg aattgtagcc attagcatgc
    12241 ttccagtttt ctcggtcagg gaccgagcgt taatcgaaat tcggttacca gcgctctttc
    12301 agcaggttcg gctgaatccc attaatcaaa tgggtattgc tgaacggctg tttgattccc
    12361 gcctcaaatt cttcgcggaa atatttgatg gcgctctgta acggctccac tgcaccaggt
    12421 gcgtgggcac agaaagtttt acccgggcct aagaatcgac acagttgctc aagtgtttcg
    12481 atatcgcccg gctggccttc gccacgctcc agcgcacgca gaattttcac gctccacggc
    12541 agaccgtcgc ggcacggcgt acaccagccg caggactcac gggcgaaaaa ctcttccagg
    12601 ttacgcacca gcgacaccat gttgatctca tggtcaaccg ccatcgccag cgccgtaccc
    12661 agacggctgc ccgctttacc gatactttcg aattccatcg gcagatcaag gtgcgcttcg
    12721 gtcaggaagt cagtccccgc gccgcctggc tgccaggctt taaatttcag accatcacgc
    12781 ataccaccgg cgtaatcttc gaggatctcg cgtgcggtgg tgccgaacgg cagttcccac
    12841 agtcccggat ttttcacccg accggagaag cccatcagct tggtgccagc atctttactt
    12901 ttcgagatgt tctgatacca ctccacgccg ttagcgagga tcgccggaac gttacacagg
    12961 gtttcgacgt tgttgacaca ggtcggtttg ccccatacgc cggaggttgc cgggaagggg
    13021 ggtttcgagc gtgggttagc acgacgccct tccagggagt tgattaacgc tgtttcttcc
    13081 ccgcagatgt agcgccctgc cccggtatgg acgaacagtt cgaagtcgaa acctgttccc
    13141 ataatgtttt tgccaagcaa gcccgcttca gtggcttcgg caatggcgcg gcgcagattt
    13201 actgccgctt cgatatattc gccacgcagg aagatgtagc cacggtaagc tttcagcgca
    13261 aacgcggaga tgagcatacc ttccaccagc aggtgcggca gttgctccat caacaggcgg
    13321 tctttatagg tgcccggctc catttcatcg gcgttacaca gcaggtaacg gatgttcatg
    13381 gattcgtctt tcggcatcag gctccacttc aggccagtcg aaaagcccgc tccgccgcgc
    13441 cctttcagac cagcgtcttt tacctgatta acgatttcgt ccggagacag cccggtcagc
    13501 gccttacgcg ccccttcgta accgtttttg ctgcggtatt cgtccagcca cactggctgt
    13561 ttgtcatcgc gcagacgcca ggtcagcgga tgcgtttcgg gagtacggat aatgtttttc
    13621 atttataccg ctccagcagt tcagggatcg cttccggggt cagatgcgcg tgagtgtcct
    13681 catcgatcat catgtttggc cctttatcac agttccccag gcagcaagtt ggcagcagcg
    13741 taaagcggcc atcaaatgtc gtttgccctg gtttgatgtt cagctttttc tcgagcgccg
    13801 cctgaatacc ctgatacccg ttgatatgac agaccacgct gtcgcaataa cggatcacat
    13861 ggcgaccaac cggctggcgg aagatctgac tgtagaacgt tgccacacct tcgacgtcgc
    13921 ttgccggaat acccagcaca tcggcgatcg cgtggatcgc accatccggc acccagccac
    13981 gctgcttctg aacgattttc agcgcttcaa tggacgccgc acgcgggtct tcgtagtggt
    14041 gcatctcgtg ctcaatcgct tcacgctctg ccgcactcag ctcaaaagcc tcggtttgtg
    14101 gttgttgatt ctcgtgcata attagcggtc cacatctgac ataacaaaat cgatactgcc
    14161 cagataaaca atcaggtcag acaccaggct gccgcggatc gccgccggaa tttgctgcaa
    14221 atgcgcataa ctcggcgtgc ggatacgagt gcgataactc atggtgctac cgtcgctggt
    14281 caggtagtaa ctgttgatcc ccttggtcgc ctcaatcatc tggaaagatt cattggcagg
    14341 catcaccggc ccccacgaca cttgcaggaa gtgggtgatc agggtttcga tatgttgcag
    14401 cgtgcgctct ttcggcggcg gcgtggtcag cgggtgatcc gctttgaacg ggccttccgg
    14461 catgttgttg aggcactgct caagaatgcg cagactctgg cgcagctctt ccactttcag
    14521 cattacgcgg gtgtagcagt cagaaacacc accaccaacc gggatttcaa agtcgaagtt
    14581 ttcatagcca gaataaggac gcgccttacg cacgtcgaag tcgatcccgg tagcacgcag
    14641 gcccgcgcca gtggtgcccc actccagcgc ctctttcgcg ccataggcgg caacgccctg
    14701 ggaacgacct ttcagaatgg tattttgcag cgccgctttc tcgtaagacg ccagacgttt
    14761 cggcatccag tcgaggaact cacgcagcag gcgatcccag ccgcgcggca ggtcgtgcgc
    14821 tacgccgcca atacggaacc acgccgggtg catacggaaa cccgtgatcg cttccaccag
    14881 atcgtaaatt ttctgacgat cggtaaaggc gaagaacacg ggcgtcattg cgccgacgtc
    14941 ctgaataaag gtcgagatgt acagcaggtg gctgttgata cggaacagtt cggagagcat
    15001 aacgcgaatg acgttaacgc gatccggcac ggtgatcccg gccagtttct ctaccgccag
    15061 cacgtaaggc atttcgttaa cgcagccgcc gaggtattca atacggtcgg tatacggaat
    15121 gtagctgtgc caggactggc gctcgcccat tttctccgca ccacggtggt ggtagccgat
    15181 gtctggtacg cagtcgacaa tctcttcgcc gtcgagttgt aaaacgatac ggaaagcccc
    15241 gtgcgccgac gggtggttcg gaccgaggtt gaggaacatg aagtcctcgt tttcggtgcc
    15301 gcgcttcatc ccccactctt ccggtttgaa ggtcagggct tccatctcca gatcctgttt
    15361 ggctttggtc agctcaaacg gcgagaattc ggtagcgcgc gccgggtaat ctttacgcag
    15421 cgggtgacct ttccaggttt gcggcatcat gatgcggcgc aggttcgggt gaccgtcgaa
    15481 agtaatgcca aacagatccc aggtttcacg ctcataccag ttagcgttcg ggaacagttt
    15541 ggtgaaggtc ggtacgtgca ggtcgttttc tgccagcgcc accttcagca tgatgtcgcg
    15601 gttacgatcg atagaaatca gatggtagaa aacggaaaaa tccgcggcag gtaacccttc
    15661 gcggtgtgtg cgcagacgtt cgtccatgcc gtgtaagtca aacagcatga cgtaaggttt
    15721 cggcagtttc tttaagaaat cgccaacttc cagtaattgt tcacgcttga tccacacaac
    15781 gggaaccccg gtgcgagtcg cctgaacagt aaaggcatcc ggcccaaaac ggttgcgcag
    15841 ttcgccaatc accggatcat caagatgatc acgggtctgc caggcgggtt cttgcgcggt
    15901 taagtcggtc atattgttca ccattgcaaa tggtccgtgg tgactgtcgg cgcagtaagc
    15961 ttcgcgaaat agatttagga ataagcgaag aaaaatccct ttgccgacag gcgcaaatta
    16021 aatctcgtca ggtgtacgca ggttggttac ggcaatgcgt tcaccgcgct tgcgttcgcg
    16081 ctctgattgc atattggcgc gataaacgcc ctgatcgcca accacccagg agagcggacg
    16141 acgttctttg ccgatagatt cctgcaacag catcagtgcc tgcatgtacg cttcaggacg
    16201 cggcgggcag cccgggatat acacatcaac cgggatgaat ttatcgacgc cctgcacaac
    16261 ggaataaata tcgtacatac caccagagtt ggcacaggca cccattgaga taacccattt
    16321 tggttccagc atctggtcat acagacgctg aataaccggt gccattttgg taaagcaggt
    16381 tcctgcaacc accatcaggt cagcctgacg cggcgaagca cgcaatactt ctgcgccaaa
    16441 acgcgccacg tcatgcaccg cggtaaacga agtcaccatc tcaacgtaac agcaggaaag
    16501 accgaagtta tacggccaaa ttgagttttt acgaccccag ttaaccatgt cattgagctt
    16561 gcccataaac acgtttttgt taacttcttg ctccagaggg tcggttacga tctcctgctt
    16621 ttgcaggggg taacggtcgt tctcaccgtt gggatctatg cgggtgagcg tataatccat
    16681 cttaatgcct cgcggttagc gttgacgatt agcgatactg ttcgtttccg ggttcatacg
    16741 ctcgcggcgt gaacgcgcgg gcgtccagtc cagcgcgcca atacgcacca gataaaccag
    16801 acctgccagt aacacaaaaa taaaaattgc agcttccaca aagcctaccc agccgctttc
    16861 gcggatagag gttgaccatg cgaacagata cagcgcttca acgtcgaaga taacgaagaa
    16921 catggccacc agataaaact tggcggacag gcgtaagcgg gcggagccga ccgagtcgat
    16981 accggattca aacggcacgt ttttcgacct cgcgcgtgcg cgaccgccta aaaaccaacc
    17041 gcctaccagc atcaggcaac acaggccaat ggcaacgata agaaagatag cgaatgccca
    17101 gtgatgagcg atgacttcag tggatgttga catactcatt gcttactcat caaaagtagc
    17161 gccagattca ctgctcttca cggcagatgg acgccacatc gattcatggg gaggaataaa
    17221 aaaaacctta caatcactgt agaaattctt ttatacagct aattgatgtg gtcttttact
    17281 cctttctata accttttgtc aactttaaca aaagtttctt cacattagtt tacataatat
    17341 caacaccatt agcatttaat gccctttcac gccagatcct tgacgactcc aggataatta
    17401 gatgttgttg aatcgtgtcc gttgtgaagc aatggaaaaa atacgggtct attttgacag
    17461 gaatttgtgt cgattcctcc ccccaaaaga gagtattttc ttgatctgtg acacgctttt
    17521 gtcattccat aacaaaaacg cagcaacaaa tttacgtatt tttaacatca ttgtagcagg
    17581 tgattttttt caggcgatta tttgtgcgtt cgggacgtga atctctggtg attgaaaaat
    17641 gaacagtttt gtacgttctg cactatgcga ggaaggattt ttactaaaaa aaagccgctg
    17701 gggtttaaaa cacccccagc ggctcgtttt tacactattg tctcaggaat tatctatcgt
    17761 ccgtcgatta ctcgatatcc ctttcaatca acaatgaatc atccccttcc ggagcagaca
    17821 ttgggctgta ttgccacgga ttatggtagc tctccatcgc ctgatagatc acctgggcca
    17881 gctcattatt actggacggg tcatagcaca gcaaatattc ggtatcaggt agcggcggta
    17941 agccatctac tccactcagc acacgcaggt ccgggctcat catttcaatc ggccttgccg
    18001 tcacgccaag accggctttc actgccgcac gaacggccgg aagcgtcgag gcgacataag
    18061 ccagtcgcca tggaatatct gctttattaa gcgtcgccag caccatatcg cgaaacggac
    18121 ttggatcatc cagcaataca agagggatcg gctcgccttt ttgcaatacg tattccgctg
    18181 cgcagtacca gtgtgttggc gaggtacgca ggttcaatgc cttaaacgct gaaggacgat
    18241 gggtggttac catcaaatcc acttcctggg attccagcat ttctgccata taggcattac
    18301 gtttaacgcg gacatccagc gctaatttcg gataaaccga actcacgcga tttaacagga
    18361 aaggtaagat cgtatcggca gattcatctg aagcaccgat agttaacacg ccctgaagat
    18421 tactgaacat taatgatgag caggcctcat cattaaaacg cagtattttc ctggcgtaac
    18481 caagaagttg aatgccatgt tcagttaaca gtttgttgcg accgtgacga gcgaacagtt
    18541 ctttcccaac gagttgttcc agacgctgca tttgctgact tacggcggac tgagtacgac
    18601 acacagcggc agctgcggca gcaaaagtgt tcagatcggc aacagcaaca aatgttctca
    18661 gcagatcgag gtcgaggtta attatcggac gatttgcact tatcatatat tatcacttac
    18721 tggcggctca tactgagctg gttaatgctg tgcacacaca aacaagcaat tccatttgta
    18781 atgtgcctcc ctggcagttt catccggaac ccggacgaaa gtaaaaatgc atatgagttg
    18841 cactaaaaaa gcgactcaca ttgttccgtt ataatgcctg aagtagatca cagaatatat
    18901 cttcagggat cgcatatcta ttaagttact cactcttttc tatttatgac atgcgcgtgt
    18961 ttgtataaat gtaaatgtga gtccttgttc cactctcgtg cagcatcgct ggtcatacgc
    19021 gaacacgtac aaacagcaat ggtgaggcgg catcaagagc agggatccgt catttatgcg
    19081 agcattttac cccaaaaacc ttttatttat aagggtcatt gcgaattatc tgatgcaaag
    19141 ttatgttatg ttaggcaaag taatcttcta ttattaataa gcacatcaaa acctttttga
    19201 atattaaata ataattaatt agcatcatcc tcattcatta attccgctta acaatagttt
    19261 cacaccttgc ccccattgcg acacctcgga aatcatcacg ctgttaaact ctccgtaatg
    19321 cggaaatatc atcatcgcgt ttcattagtg aattcttaaa tgaggcattt tcacacaatt
    19381 atcttacaga taaaaaaacc agacttgcaa ttaagaatca gaacaattca ctatataaca
    19441 ttgcatgcaa agcatataca cctcattatt ttgtcattat taagttatta acagcacaat
    19501 cgagccttcc cctctggcaa aatcttattc tgcagacctt caaaacaccg tcctggggga
    19561 gtacattgtt ctaagctgac ttccacggca gggagtggcg ataacagcaa aaaaggtcaa
    19621 gattcatgtc ccccattgaa aaatccagca aattagagaa tgtctgttat gacatccgtg
    19681 gtccggtgct gaaagaagca aaacgcctgg aagaagaagg taacaaggta ctgaaactga
    19741 acatcggcaa cccagcccca ttcggttttg acgcgccaga tgaaatcctc gttgacgtga
    19801 tacgcaacct gcctaccgct caagggtatt gcgattccaa agggctttac tccgcgcgta
    19861 aagccatcat gcagcactac caggctcgtg gcatgcgtga tgttaccgtg gaagatattt
    19921 acatcggcaa tggtgtatcg gagcttatcg ttcaggcaat gcaggcattg ctgaacagcg
    19981 gggacgaaat gttggttcct gcaccagatt acccactctg gaccgcggcg gtttcgcttt
    20041 ccagcggtaa agcggtgcat tatctttgcg atgaatcctc tgactggttc ccggacctcg
    20101 atgatattcg cgctaaaatt acgcctcgta cgcgtgggat cgttattatc aacccaaata
    20161 acccaaccgg cgcggtatat tccaaagagc ttttaatgga gattgtggag attgcacgtc
    20221 agcataatct cattatcttc gccgatgaaa tttatgacaa aattctctac gacgatgctg
    20281 agcatcactc aattgcgcca ctggcacctg acctgctgac cattaccttt aacggactgt
    20341 cgaaaacgta ccgcgttgca ggcttccgtc aggggtggat ggtgttgaac gggccgaaaa
    20401 aacacgccaa aggctacatc gaaggtctgg aaatgctggc ttcaatgcgc ctgtgtgcta
    20461 acgttcctgc gcaacacgcc attcagaccg cgctgggtgg ttatcagagc atcagtgaat
    20521 ttattacccc tggcggtcgt ctttatgagc agcgtaaccg cgcgtgggaa ctgatcaacg
    20581 atattccggg cgtttcctgc gtgaaacctc gtggtgcgct gtatatgttc ccgaaaatcg
    20641 acgccaaacg ctttaacatt cacgacgatc agaaaatggt gctggatttc ctgttgcagg
    20701 aaaaagttct gttggtgcaa gggacggcat tcaactggcc gtggccggat cacttccgca
    20761 ttgtcacgct accgcgtgtc gatgatatcg agctgtcttt gagcaagttc gcgcgtttcc
    20821 tttctggtta tcatcagctg taatcttaat ttcactgccg gagattgcat ccggcagcgt
    20881 tatcccgcca caatgacctg atgatgtcat catacgtaag gtcactatga aacagagcca
    20941 tttttttgcc catctctccc gcctgaaact cattaaccgc tggccgctaa tgcgcaacgt
    21001 gcggacggaa aatgtgtccg aacacagttt gcaggtagcg atggtcgccc atgcgctggc
    21061 agctatcaaa aatcgaaaat ttggcggtaa tgtcaacgcc gaacgtatcg ctttactggc
    21121 gatgtaccac gatgcctcag aagtgctcac cggcgatctc cctactccgg tgaaatactt
    21181 caattcgcaa atcgctcagg agtacaaggc tattgaaaaa atcgctcagc aaaaactggt
    21241 cgatatggtt ccggaagagc tgcgggatat ctttgcgccg ttaattgacg agcatgcata
    21301 tagcgatgaa gaaaaatcgc tggtgaaaca ggcagatgca ctgtgtgcat atctgaaatg
    21361 tctggaagaa ctcgcggccg gaaataatga attcttgctg gcaaaaacgc gactggaagc
    21421 gacgcttgaa gcgcgtcgca gccaggagat ggactacttc atggaagtat ttgttcccag
    21481 cttccatctt tcgctcgatg agattagcca ggattcaccg ctgtaagcag ccggagtctg
    21541 cgtcgcatca ggcaataagc gccggatgcg acatcaggct cttgtcaaaa cggaaacagc
    21601 atcgggatca tcaccacaca aaccgccatc acgataatgg tgaacggtac ccccaacttc
    21661 acaaagtcac tgaagctgta atttcccgga cctaaaacca gtgtgttaac aggtgaagaa
    21721 accggtgtca taaaggcggc ggatgctgcc atcgccacga ccatcgcgaa tggataaggc
    21781 gacaccccca tcgttttggc agcagccagc gcaatcggag ccatcaacac cgccgtcgcg
    21841 gtattagaga taaatagccc aataaccgcc gacaagacaa acaaacaccc cagcatcata
    21901 tgtggcccgt aaccaccgcc aatgtccatc agccctttca ccgccagcgc gacacctccc
    21961 gttttctgta atgccacagc aaacggcatc atcccaacga tcaaaataat gctcggccag
    22021 tgaatggatt tataggcgct ttcagcatct atacagcgga atttccccat cagcaggcag
    22081 gcgatgatag cggcaacagg attaggaatt tcatctgtca gcattaacgc caccattagc
    22141 accagacaga aaatggcatg gggtgcctgg ctgtgcgcgg gcgatgcttc actcacctct
    22201 tccggtaagt tcagcgctac gaagtcgcgg ccctgtttgg ccagcatacc gatcagtttc
    22261 cagttaccca caaccaggat gatatcgccc agcagcagag gctcatccgc cagcgaacct
    22321 tccagcgcca cgccattgcg ctttagcccc accacattca gtccgtagcg ggtacgaaaa
    22381 ccaatttcgc gcaccgattt accaatcagt tctgactcag gaattaatga aatctctgcc
    22441 atgcccacat caagggcctg gtcagaaaaa tactcgccgc gcagtaccat cggctccagc
    22501 aattgctcac tacaaaattg ccggagatcg acatcagccg cagacatatc aataagcaaa
    22561 acgtcacgcg cgcgaaattc agaaacccca ttaacgttca cgataacgcg acgaaaacgc
    22621 cgccagcgtt caacaccgat gacgttagcg ccataacgct cacgtaattt gagatcatcc
    22681 agccgttgac caatcattgg cgatccgggg cgaatagcca gacgtcgcgc acgcccggtc
    22741 agtcgatatt cacggataag atcgcgaaag gttcgacgcg tccagccttc gcgctgcggg
    22801 gtctgggtat cccctttcag catgaaacgc atcactaaca tatacaaaat acccagcacc
    22861 agcacgacca ggccaatagg tgttacgcta aagaaactga agccgtgata gccttcacgc
    22921 agcaattcac tgttgactac caggttcggc ggcgtcgcca ccagcgtcat catgccgctt
    22981 atcagcccgg caaaactcaa cggcatcatc agacgtgacg gcgaagtttg catacgcatg
    23041 gcaacgctta acaccacggg aataaagata gcgacaacgc cggttgaact cataaacgcg
    23101 ccaagccccg cgacggtcag catcaacaaa accaacattt tgatttcact gttgcccgca
    23161 actttgacca gccatgttcc cattacggtg gcaacaccgg tacggaccaa accatcgcca
    23221 ataataaaca aggcggcaat caggacaacg ttaggatcag aaaagccgga aaatacttcg
    23281 gggactgtca gcgttccgct taatgcaaac gcgacaataa caaacaaagc gaccgcatcc
    23341 atacgcactc tgcccgtcgc aaacaagaca atggcaacgg ccagtaatga aagaacccaa
    23401 atcaattcac cgttcacaac ttatccttgt taattgaggg agatgtcttg attctgccat
    23461 aaaaaagccc cgacgagacg gggctaaatc atgatcaggt gtttcactga ataataacat
    23521 cgccatttgg ctgtttggtc acagtaattt gctccagact gtggaggacc aaatcgacct
    23581 cattcaggcg cggggtatct gccggagcgt taaccgcaat gacatgacaa cccgccgcca
    23641 ggccagaaag cacgccagcg gagcatcttc caccaccaca cactcctgcg gcgcaagccc
    23701 cagcagctgc gcgcctaaca gatacgcatc aggttctggt tttccgcgct tcactcgctc
    23761 agcggttaca aacacctctg gtgcgggaag cccagctatt ttatggcgcg ctcgcgctac
    23821 cggcatggag ccagaagtca caatggccca cggaatacct gctttattca aatgactgag
    23881 taaggcgatt gcccccggaa gcgggtaata ccttcggttt ccgtggcctc gatgtgctcc
    23941 agacgcgtaa actcggcggc aatatcagcc tcggatttgc ccgccataaa atggcgcaga
    24001 gaggtgatcg cctgtttacc gtgaatgaaa gccagcacct cttccggcgc taacccatga
    24061 cgtctggccc agttgctcca cgcccgttct accgcaggca gggaatccac cagcgttcca
    24121 tcaagatcaa acagaaaacc tttgcaccgc acgcgggcct cctcaggcat tgataatttg
    24181 attaatttcg ttggcgctca aatggtactg acgcgggcag gcatgccaca cattaagcat
    24241 gcgctgatat ttttcccaca ttggcgtctg ggcgttaaaa ccgtgagttc cggcgtcaaa
    24301 atgggtatag cgcccttcca cattaaccat aaagcggaca taaccgaggt aacgtgcttc
    24361 agtggcggcg tcaaagccga ggaaggtgac acgacgttca tcgatggatt gctggtcctg
    24421 caaattagac caggaaacat gcaacgcatg atacatctcc ataatgtcga tgatagtgcg
    24481 gcaggtttct tctttcagct cgccaaactc gcgatccaat tcacgcatct gtaatccgta
    24541 acctcgctca ataattgttt gcaggcgacg gtaacgttca gcatttgccg gatcgagcat
    24601 agtcatcatc ttgtactgat tagacaaaat aagacgttgc gcgttggtca tttccattgt
    24661 tgactcctgt atcactctac tacggtgaaa aaaaagaagg ctgagtatgc cttcttttat
    24721 atgcgtaatc aggggtcaat tacaaatcat caaggaaagt tttatccagt tgtttgaagg
    24781 cgcgcttaag cgtgtcagct aatgcctggt aatcaggctt gccttcaacg ggtgccaaca
    24841 cctgtccaga ctcctgcaat ttaccgcgaa cttcataaaa ccagttaagg attgcagggg
    24901 gtaatggcgt tacagaacgc ttgcccagcc accacaatcc ctgcatgggt aaacttaagg
    24961 cgaacagtgc agtggcaact gccggcccaa gcagaccgcc cagggcaatc tgccagcaga
    25021 gagtaaatac ggcgatcggc ggcataaaac ggatcgcata acgcgtcatc ttgataacgc
    25081 gattttcgac aaagaccggg gcaaggcgtt tttccagtgg ccacgtcttt gagtaatgct
    25141 gtccgcggcg aaacaagcta aaaaaattaa cagaacgatt atccggcgtt gacatgcttc
    25201 acctcaactt cacatataaa gattcaaaaa tttgtgcaaa ttcacaactc agcgggacaa
    25261 cgttcaaaac attttgtctt ccatacccac tatcaggtat cctttagcag cctgaaggcc
    25321 taagtagtac atattcattg agtcgtcaaa ttcatataca ttatgccatt ggctgaaaat
    25381 tacgcaaaat ggcatagact caagatattt cttccatcat gcaaaaaaaa tttgcagtgc
    25441 atgatgttaa tcataaatgt cggtgtcatc atgcgctacg ctctatggct ccctgacgtt
    25501 tttttagcca cgtatcaatt ataggtactt ccatgtcgag taagttagta ctggttctga
    25561 actgcggtag ttcttcactg aaatttgcca tcatcgatgc agtaaatggt gaagagtacc
    25621 tttctggttt agccgaatgt ttccacctgc ctgaagcacg tatcaaatgg aaaatggacg
    25681 gcaataaaca ggaagcggct ttaggtgcag gcgccgctca cagcgaagcg ctcaacttta
    25741 tcgttaatac tattctggca caaaaaccag aactgtctgc gcagctgact gctatcggtc
    25801 accgtatcgt acacggcggc gaaaagtata ccagctccgt agtgatcgat gagtctgtta
    25861 ttcagggtat caaagatgca gcttcttttg caccgctgca caacccggct cacctgatcg
    25921 gtatcgaaga agctctgaaa tctttcccac agctgaaaga caaaaacgtt gctgtatttg
    25981 acaccgcgtt ccaccagact atgccggaag agtcttacct ctacgccctg ccttacaacc
    26041 tgtacaaaga gcacggcatc cgtcgttacg gcgcgcacgg caccagccac ttctatgtaa
    26101 cccaggaagc ggcaaaaatg ctgaacaaac cggtagaaga actgaacatc atcacctgcc
    26161 acctgggcaa cggtggttcc gtttctgcta tccgcaacgg taaatgcgtt gacacctcta
    26221 tgggcctgac cccgctggaa ggtctggtca tgggtacccg ttctggtgat atcgatccgg
    26281 cgatcatctt ccacctgcac gacaccctgg gcatgagcgt tgacgcaatc aacaaactgc
    26341 tgaccaaaga gtctggcctg ctgggtctga ccgaagtgac cagcgactgc cgctatgttg
    26401 aagacaacta cgcgacgaaa gaagacgcga agcgcgcaat ggacgtttac tgccaccgcc
    26461 tggcgaaata catcggtgcc tacactgcgc tgatggatgg tcgtctggac gctgttgtat
    26521 tcactggtgg tatcggtgaa aatgccgcga tggttcgtga actgtctctg ggcaaactgg
    26581 gcgtgctggg ctttgaagtt gatcatgaac gcaacctggc tgcacgtttc ggcaaatctg
    26641 gtttcatcaa caaagaaggt acccgtcctg cggtggttat cccaaccaac gaagaactgg
    26701 ttatcgcgca agacgcgagc cgcctgactg cctgatttca caccgccagc tcagctggcg
    26761 gtgctgtttt gtaacccgcc aaatcggcgg taacgaaaga ggataaaccg tgtcccgtat
    26821 tattatgctg atccctaccg gaaccagcgt cggtctgacc agcgtcagcc ttggcgtgat
    26881 ccgtgcaatg gaacgcaaag gcgttcgtct gagcgttttc aaacctatcg ctcagccgcg
    26941 taccggtggc gatgcgcccg atcagactac gactatcgtg cgtgcgaact cttccaccac
    27001 gacggccgct gaaccgctga aaatgagcta cgttgaaggt ctgctttcca gcaatcagaa
    27061 agatgtgctg atggaagaga tcgtcgcaaa ctaccacgct aacaccaaag acgctgaagt
    27121 cgttctggtt gaaggtctgg tcccgacacg taagcaccag tttgcccagt ctctgaacta
    27181 cgaaatcgct aaaacgctga atgcggaaat cgtcttcgtt atgtctcagg gcactgacac
    27241 cccggaacag ctgaaagagc gtatcgaact gacccgcaac agcttcggcg gtgccaaaaa
    27301 caccaacatc accggcgtta tcgttaacaa actgaacgca ccggttgatg aacagggtcg
    27361 tactcgcccg gatctgtccg agattttcga cgactcttcc aaagctaaag taaacaatgt
    27421 tgatccggcg aagctgcaag aatccagccc tctgccggtt ctcggcgctg tgccgtggag
    27481 ctttgacctg atcgcgactc gtgcgatcga tatggctcgc cacctgaatg cgaccatcat
    27541 caacgaaggc gacatcaata ctcgccgcgt taaatccgtc actttctgcg cacgcagcat
    27601 tccgcacatg ctggagcact tccgtgccgg ttctctgctg gtgacttccg cagaccgtcc
    27661 tgacgtgctg gtggccgctt gcctggcagc catgaacggc gtagaaatcg gtgccctgct
    27721 gctgactggc ggctacgaaa tggacgcgcg catttctaaa ctgtgcgaac gtgctttcgc
    27781 taccggcctg ccggtattta tggtgaacac caacacctgg cagacctctc tgagcctgca
    27841 gagcttcaac ctggaagttc cggttgacga tcacgaacgt atcgagaaag ttcaggaata
    27901 cgttgctaac tacatcaacg ctgactggat cgaatctctg actgccactt ctgagcgcag
    27961 ccgtcgtctg tctccgcctg cgttccgtta tcagctgact gaacttgcgc gcaaagcggg
    28021 caaacgtatc gtactgccgg aaggtgacga accgcgtacc gttaaagcag ccgctatctg
    28081 tgctgaacgt ggtatcgcaa cttgcgtact gctgggtaat ccggcagaga tcaaccgtgt
    28141 tgcagcgtct cagggtgtag aactgggtgc agggattgaa atcgttgatc cagaagtggt
    28201 tcgcgaaagc tatgttggtc gtctggtcga actgcgtaag aacaaaggca tgaccgaaac
    28261 cgttgcccgc gaacagctgg aagacaacgt ggtgctcggt acgctgatgc tggaacagga
    28321 tgaagttgat ggtctggttt ccggtgctgt tcacactacc gcaaacacca tccgtccgcc
    28381 gctgcagctg atcaaaactg caccgggcag ctccctggta tcttccgtgt tcttcatgct
    28441 gctgccggaa caggtttacg tttacggtga ctgtgcgatc aacccggatc cgaccgctga
    28501 acagctggca gaaatcgcga ttcagtccgc tgattccgct gcggccttcg gtatcgaacc
    28561 gcgcgttgct atgctctcct actccaccgg tacttctggt gcaggtagcg acgtagaaaa
    28621 agttcgcgaa gcaactcgtc tggcgcagga aaaacgtcct gacctgatga tcgacggtcc
    28681 gctgcagtac gacgctgcgg taatggctga cgttgcgaaa tccaaagcgc cgaactctcc
    28741 ggttgcaggt cgcgctaccg tgtttatctt cccggatctg aacaccggta acaccaccta
    28801 caaagcggta cagcgttctg ccgacctgat ctccatcggg ccgatgctgc agggtatgcg
    28861 caagccggtt aacgacctgt cccgtggcgc actggttgac gatatcgtct acaccatcgc
    28921 gctgactgcg attcagtctg cacagcagca gtaacctcgt catcatccgc agctttgcgc
    28981 tgcggatatc tgaaccggaa ataatcacta tttccggttt tttattctct taatctgcat
    29041 taatcctttc tgattatctt gcttaactgc gctgcatcaa tgaattgcgc catttcactt
    29101 tacatactta ccactttgtt ttgtgcaagg gaatatttgc gctatgtccg caatcactga
    29161 atccaaacca acaagaagat gggcaatgcc cgatacgttg gtgattatct tttttgttgc
    29221 tattttaacc agccttgcca cctgggtagt tccggtgggg atgtttgaca gtcaggaagt
    29281 gcagtatcag gttgatggtc aaacaaaaac acgcaaagtc gtagatccac actcatttcg
    29341 cattctgact aacgaagcag gcgaacctga gtatcaccgc gtacagctgt tcacgacggg
    29401 cgatgaacgc ccgggcctga tgaacttccc gtttgaagga ttaacctcag gatcgaaata
    29461 cgggacagct gttggcatca tcatgtttat gctggtgatt ggcggcgcgt ttggcattgt
    29521 gatgcgtaca ggaaccattg ataacggtat cctggcgctt attcgccata cccgcgggaa
    29581 tgaaattctc tttattcctg cgctgtttat tctgttttca cttggcggcg cggtatttgg
    29641 tatgggagaa gaggccgtcg cctttgccat tatcatcgca ccgctaatgg tccggctggg
    29701 ctatgacagt attaccaccg tcctggtgac ctatattgcc acgcaaatcg gttttgccag
    29761 ttcgtggatg aacccgtttt gtgtggtcgt tgctcagggg attgccggcg ttccggtgct
    29821 ttctggctcc gggttgcgca tcgtggtgtg ggttatcgcc actatgattg gcctgatctt
    29881 taccatggtg tacgcctcac gagtgaaaaa gaatcctctt ctgtcacgcg tgcatgagtc
    29941 cgaccgcttc tttcgtgaaa agcaggcgga tgttgaacaa cgtccgttta cctttggtga
    30001 ctggctggta ttgattgtcc tgaccgccgt aatggtctgg gtgatttggg gcgtgatcgt
    30061 taatgcctgg tttattccag aaattgccag ccagttcttc accatgggtc tggtgattgg
    30121 catcatcggc gttgttttcc gccttaacgg catgacggtt aataccatgg cttcatcctt
    30181 taccgaaggg gcgcgaatga tgatcgcccc tgccctgctg gtgggtttcg ccaaagggat
    30241 tttgctgctg gtcggtaatg gtgaagcggg tgatgccagc gtgttaaata ccatcctcaa
    30301 cagcattgcc aatgccatta gcggtctgga caacgcggtc gcggcctggt ttatgttgct
    30361 cttccaggcg gtatttaatt tcttcgtgac gtccggttct ggtcaggcgg cgttaaccat
    30421 gccgttactg gcaccgcttg gcgatctggt cggtgttaac cgtcaggtta ccgtgctggc
    30481 attccagttt ggtgatggct tcagtcacat catttaccca acctcagctt cgttaatggc
    30541 aactctcggt gtgtgcaggg tggacttccg taactggctg aaggtgggcg cgaccctact
    30601 tggactgctg tttattatgt ccagcgtcgt agtgatcggc gctcagttga tgggctacca
    30661 ctaaaaatgt taagagccgc aattgcggct cttttttcat tctgccgttt cagtctctac
    30721 ggcttcattt ttggcattgc gcttcatcca caacgccagc gctttcagcg agtctggagt
    30781 gaactcatcg cagcgtgcgg tgatttcttc cggcgtcagc cagcaaactt cactgacttc
    30841 atcttcctgt agggcgaagg gaccgtgaga gacgcagctg aacaacgcgc cccagacacg
    30901 gcaattttta tcttcgaaat agaactgccc gtgctcggca aaggggacac cggcaatgcc
    30961 caactcttct tccgcttcgc gacgcgcgga ttccagcagt tgctcatcgg cctgaaccac
    31021 accgcctgcg gtcgcatcta acatgccggg taaaaagtct tttgtctcgg tacgacgctg
    31081 gaccagaatt ttgcccatgc catcatgcac gacgatgtaa gttgcacgat gacgcagaca
    31141 ctgtgcccgc atttgttccc ggctggcttg tgcaatgact tcgttctctt cattgacaat
    31201 atccacccat tcagtacttg ccaaacgacg ctgttccatt atcaggaaac cttctttttc
    31261 tggcgctctt acggcgcatt ttgaattgtg ggtaaattac ggattaatcg cgacctgcgc
    31321 aatgatactt tgatcattga gtgcgattac actaagtacg tcattatcca gcatgccata
    31381 actcgccgga ttaccgcctt tcggaatact caccgagccg gggttgaagt ggaaaatctc
    31441 cccccgctgt tctgccactg gcagatgggt atgaccgtac accagcacat cgttctggtt
    31501 taaagcaggt agattttccg ggccaaaaag atgaccatgc gtcaaaaaca gacgttgttt
    31561 ttccagtaat acctgttgcc acggcgcggt tatcgggaaa tgcagcagca tttgatccac
    31621 ttcgctgtcg cagttgccgc gcacagcgat aaccttatgt gccacttcat taagccgttc
    31681 agcgactttg gctggcgcgt aaccctccgg taaagcatta cgcgggccgt gattcaacac
    31741 gtcgccaagg atcaccagcc actgggcacc gctttgggca aacaactcca gaacacgttc
    31801 cgtcgccggt aacgacccat gaatgtccga tgcaaacatc agtttcatca ctcactcctc
    31861 gtcgaaaaaa cgtaagctat gatactggat tctgtcgttg ctatcagcca gattgcttcg
    31921 ccgaaagtgc aataaaacgc tggacagacg ctcgctgcca ctggaatgtc gcataatcca
    31981 ccaggcgttc tggcacctca tcgccatgta gtaccaggcg gttaatcatt agcgccagat
    32041 cagtatcagc aatgcaccat tcaccaaata aattcggctg acccagtgct aacaaatgtt
    32101 ctgccatcgc gaacagtttc tctgcactgg cttttccctc ggccgttagt ggcgcttttt
    32161 tcgcccccgc aaagacaaca tccgtcggac gctcttcgcg gatgggcatc agatcgctgc
    32221 gcagccaggc ctgaatccgt cgtgcacgcg cacgattttc taaatcaagc ggataaatac
    32281 gttcccaggt cggtggcgca aatcgatctt ccagatactc cgcaatggca gaagattcac
    32341 tcaactcaaa atcatcgatt tgtaataacg gcacacggcg tgtctgaccg taaccttgcc
    32401 acgtcggctt caaatgttca ccgctgtcga ggtcgatggt cttgatatga aacgacaggc
    32461 ctttttcctg caacgccacc caggcggata acacataagg ggagaaaaag tgggcatctg
    32521 accaaagcgt gatagcgggt ttactcatta tgtcctcggc aataaagggg gctggctatc
    32581 aaactataga gcctttttgt cactgtcacg tgacgaatac tcacgtaagt cgccgcgctt
    32641 tctatacttg tggtgttcag atgattaaac aaccggagct gcaatgatcg atctctattt
    32701 cgccccgaca cccaatggtc acaaaattac gctgtttctc gaagaagcag agcttgatta
    32761 tcgcttgatt aaggtagacc tggggaaagg cggtcagttt cgtccggaat ttttgcgcat
    32821 ttcgcccaac aacaaaattc cggcaattgt tgatcattct cctgccgatg gcggcgaacc
    32881 gctaagcctc tttgagtctg gtgccatttt gttgtatctg gctgagaaaa caggactctt
    32941 tttgagtcat gaaacgcgtg agcgcgccgc cacattacag tggttattct ggcaggtagg
    33001 cggactgggg ccgatgcttg ggcaaaatca tcattttaat cacgcagccc cccaaaccat
    33061 tccttacgct attgaacgtt atcaggttga aactcagcgt ctttaccatg tactaaacaa
    33121 gcggctggaa aactcgccct ggctgggagg cgagaactac agcattgcgg atattgcctg
    33181 ctggccgtgg gttaatgcct ggactcgcca gcgaattgac ctcgcaatgt atccggcagt
    33241 caagaactgg catgagcgga tccgttcgcg ccctgccacc gggcaggcac tgctaaaagc
    33301 acaactcggt gatgagcgtt cggatagtta acagaaacag gttctcgtgt attatttcat
    33361 cctaagtaaa acaacggaga acctgcaatg gcacaacctg ccgctattat tcgtataaag
    33421 aaccttcgtt tacgtacgtt tatcggaatt aaggaagaag aaattaacaa ccgtcaggat
    33481 attgttatca atgtgacgat ccactacccc gccgataaag cgcgcactag cgaagatatc
    33541 aacgatgcgc tgaattatcg caccgtaacg aaaaacatta ttcagcatgt agagaataac
    33601 cgtttctctt tgttggaaaa attaactcag gatgtgctcg atatcgcacg tgaacatcac
    33661 tgggtgacgt atgctgaagt ggagatcgat aaactgcacg cgctgcgcta cgccgattcg
    33721 gtatccatga ccttaagctg gcagcgttaa ccgccatatc gggaggctgc atgaacatag
    33781 tgatcaccgg agggacggga ttaattggtc gccatttgat tccgcgtttg ctggagctgg
    33841 gccatcaaat cacggtagtg acgcgtaacc cacagaaagc cagttccgtt ctcggccctc
    33901 gggtgacgct atggcaaggg ctagccgatc aaagcaacct caacggcgtt gatgcggtaa
    33961 tcaacctggc cggagaaccg attgctgata aacgctggac tcacgagcaa aaagagcgtc
    34021 tctgccaaag ccgctggaat atcacgcaaa aactggtcga tttgattaat gccagcgaca
    34081 cgccgccgtc ggtactcatt tccggctcgg caacgggcta ttatggcgac ttaggtgaag
    34141 tggtggttac cgaagaggaa ccgccgcata acgaatttac tcataaactc tgcgcccgct
    34201 gggaagagat tgcctgccgg gcgcaaagtg acaaaacgcg agtgtgcctg ctgcgaaccg
    34261 gcgtggtact ggcaccggac ggcggtattc tcggtaaaat gctgccaccg tttcgtcttg
    34321 gcctgggcgg gccgattggt tccggtcggc agtatctggc ctggattcat attgatgata
    34381 tggtcaacgg cattctctgg ctgctggata acgacctgcg cgggccattt aatatggttt
    34441 cgccctaccc ggtacgcaat gaacaatttg cccatgcgct cggtcaagcg ctgcatcgcc
    34501 cggccatttt gcgcgtcccc gcaaccgcca ttcggctgtt aatgggcgaa tcttcaatac
    34561 tggtattagg tggacaacgc gcgctgccta aacggctgga agaagcgggt tttgcgtttc
    34621 gctggtacga tttagaagag gcgctggcgg atgtcgttcg ctgatgtggt ttacagcaaa
    34681 catccgccag ttaactcccg gtgttacagg attagtggct ttgcgcgata agatcgtctg
    34741 gtgaaagtcg ggtcaccatc ataactaact ctctgtctaa acctctatcc agcatctcct
    34801 gagcaatacg cagggcttct tcgtgtttgc cctgcattgc gccttcttca cgtaatctgt
    34861 cagcaatggt catcagtttc tccttttctt gtggtgcgcg ttctgttatc tcaccaataa
    34921 atgcacggaa acgctgggca tcccctgttt gtaatacgta attaaacagg gcttttagct
    34981 gtctgtcatt agtgttccct gtaactagca gcgaaacaat ttggtcgact aatcccaaca
    35041 gatcgcgctg acgaatatgt ttctgaatta actccaacag cgccattttg cggtgttgca
    35101 taatctcgtc atccggcacc acggtaatat ccaccaacgg aaacgctgat gaatatattt
    35161 tgcgggctat agcaggctcg gcaaattcat caagccagca gagtgaataa ggataaggac
    35221 ttctgcaacc atgataaaac agcatcggga tcaccaatgg aagctcttta tagcccgcat
    35281 caagatggtt ttgcattgcc gcaatggaat aacgcatcat gcgaaaagcc attaattctt
    35341 ccagcttact ttggtgctct atcactacat aaatataacc cactccctcc tgcgttttca
    35401 cagaccacaa gaggtcggaa taatattgcc gcaggtcttc atcaataaaa ctgtttgatt
    35461 ctagtttaag cgtcgttaaa tcacacagtt tgcgcagcgg cgcgggaaga tgaatatcaa
    35521 taaaatcccg cgcggtgtct ggatggcgta aaaaagattt aaataccgca tcatgcggcg
    35581 tggaagttgt cgatattgtc atggcgttcc gtcacccctc aaatttaaga tgacgcgaca
    35641 ataaccacga ttaacaccca atgcagcagg cagtttttat ctttggacag cgaattcaga
    35701 agtattttta gcggactgca acttttacat ttcagcggaa gctgactcgc aaattcccca
    35761 tgaaaatact ccggcagcgt tagccaccgg agaggaaatt atttcagcga tcccttaagg
    35821 aactgttgca gacgagggct ttgcgggttg ccaaataact gctccggcgc gccctcttct
    35881 tctattttcc cctgatggag gaaaatgacg tgagtagaaa catggcgagc aaagcccatt
    35941 tcgtgagtca ctaccaccat ggttttcccc tcttctgcca gttgctgcat aatacgtaac
    36001 acttcgccta ccagttcagg atcgagcgcc gaggtaggtt catcaaacag taaaacttcc
    36061 ggttccatcg ccagcgcccg cgcgatagaa acacgctgtt gctgaccgcc ggaaagatgc
    36121 accggatatt tcccctgcgc acgttcgtct atcccgactt tcgccagata cttcaccgcc
    36181 cgctcgcgcg cttcctgctt gctcaggccc aacacctgaa tcggcgcttc catgacgttt
    36241 tccagcaccg tcatatggct ccagagattg aagtgctgga ataccatcgt caggcgtgtg
    36301 cgcagtaagc gcagttgatt tttatcggcg actttgagtt gaccgtcttt gtcgcgcacc
    36361 agattgatcg tctggccatt gaccacgatc gacccttcac tcggtttttc gaggaagtta
    36421 atgcagcgca gaaaggtact tttccccgat cccgacgatc cgatgatgct tattacatct
    36481 ccggcattcg cttgcagtga gacccctttc agcacttcat gttcgccgta gcgtttgtgc
    36541 aaatcgataa cgtttaattt attctcggac atcgtattct cagtgcgttg aagaaggttt
    36601 cacatgctgc aaccagcgtt tttccgctct gcgaaagagg ctgatcagga cataagagat
    36661 gattaaatag agcaccgcgg caatgccgaa ggcggtaaaa ggttgatacg tggcggcgtt
    36721 aatatcgcgg gctattttca gcagatccgg cacagtggcc gtaaacgcca gcgcagtaga
    36781 gtgcagcatc aggatcactt cgttgctgta tgccggtaac gcaatacgca gcgcagaagg
    36841 caaaataatg caacgataca ttttaaaagt cgagaagcca taggctctgg cggcttcaat
    36901 ttccccatgc ggaaccgaac ggattgcccc agcaaaaatc tcggtagtgt aagcgcaggt
    36961 gttaagcgtc agcgccagca cggtacagtt caggccactg cggaagaaag cgttaaggaa
    37021 ttcggttccc ttaacaatct caagcgtgta catgccggaa tagaacacca gcaactgaac
    37081 atacagcggc gtaccgcgaa aaatataggt aaataaccag attggaaact ggatgtattt
    37141 attactggag acacgaccaa tcgccagaaa cagcgccagg actccgccta tcactaccga
    37201 caaaataagc agccacagag tgatcgccac accagtaaag cgataaccgt cggtccacag
    37261 cagcggtttc cagtattcat gtaagatttc gatcacaggt cagccctctt cacacccacg
    37321 gagtagcggc gctcaaggaa cagcagcaca ccattggaaa cagtggtgaa aaccaggtaa
    37381 atcacgccac agacgatggc gaaatagaac ggttcccagg tacttttgcc tgccagttgg
    37441 gtggctttga ccacatcttc caggccgagt aacgaaacca gtgcggtaga tttgaggatc
    37501 acctgccagt tgttgccaat gcctggcagg gcgtaacgca tcatcgccgg aaacatgatc
    37561 cgccgaaaca cttgcccacg agtaaaaccg aacgccgtcg ccgcctctat atgtcctttc
    37621 ggcactgcca taaaagcacc acggaacgtt tcggtaaaat aagcaccgta gataaaaccg
    37681 agagtgataa taccagcgac catcggatcg atatcaatct gcccgacgcc catcgcctcc
    37741 gtcaccgtgt ttagcgcaat ctgcaaaccg tagaaaatca gcagcatcaa cactaagtcc
    37801 ggcacgccac gaatcagcgt ggtgtaccct tcgaaaatca gccccgaaag ccgattttgc
    37861 gagagcttac cgccagcgcc aattaaaccg atgattacag cgagcactac agagctgata
    37921 gccagctcca gcgtgacgag cgcaccctgt aaaataacac ctgaaaaccc atacaacatg
    37981 ctgcctgtcc tgtcgtgtgt ggtgaattac cgcctcttgt ctccctccgg ttacccgaag
    38041 ggagcgggct ttctttcacc gatggcgatt agccaccata aacatcaaaa tcgaagtact
    38101 ttttcgctaa tttctcgtaa gtaccgtcag cgcgcatttc ggcaaaggct ttgttcagtg
    38161 cttcgcgcag ttcgttatct tctttacgca ggcccatgcc ggtccctacg ccaaacagtt
    38221 tttcatcttt aacagacggg ccaccgaatt tgtaatcttt accgacaggt tgtttgagga
    38281 aaccttcgct ggcagcgacc tcatcctgga acgcggcatc aatacgtccg gcagtcaggt
    38341 cagaataaat gttgtcctgc ccctgatacg agacgatttc aatgcctttt ggtgcccaat
    38401 gttcattacc gaacgtctcc tgggtggtgc cctgcaatac gcctacccgt ttgcctttca
    38461 gcgactcgac tgtcggctga atgtcagaat ttttcgccac caccaaacga gaatcggcag
    38521 cgtacagttt gtcggtgaag gctatttctt gctgacgttt ttccgtaatg gaaagcgatg
    38581 acatgatggc gtcaatcttc ttcgctttta aggacgggat taacgcatcc agcggatttt
    38641 cgacaaacgt acattgcgta ttgatgcgtt tgcataattc ctttgccaga tcgatatcga
    38701 agccaaccag ttcgccttgt gaattttttg attcaaatgg cgcataggtc gggtcggtac
    38761 cgatgcggat gttttgcgga atcgcagcaa atgccgcagt tgcgctggag aaggccagaa
    38821 ccagagagag cgatagcacc agttttttca taatgtcctc aactgacagt ctttttatag
    38881 ggaatattta caggtttgtt acacttatcg tgccataaaa ttgaccatca aggcaaaata
    38941 ttccgccccg ttagacgtat tatttttcat taaaaatgct taagtatgca tttcaatgca
    39001 ccacgatagt gcaacaaacc tggcgtgcac catgatgatc attccatcag gtacagcttc
    39061 ccagcgacgt atcagtcacc gtagacatta aagtcgaaat actttttcgc catcttgtcg
    39121 taggtgccgt cctgacgcag ctcgccaagc gccttattga aggcagccgt cagttcagca
    39181 tcatctttac atagccctac accggtgcca tcaccgaagt attttttgtc ttttactgat
    39241 gagccagcaa aggcgaaagc tttaccagca ggttgcttga ggaatccttc gctggcagca
    39301 acttcatctt gtaacgcagc atccagacgt cctgcagcca gatcggaata gaccaaatcc
    39361 tggttggcat aggccaccac atccacgcct ttgctacgcc aggtctcgtt agcgtaagct
    39421 tcctgggttg atccctgcag cacaccaaca tgtttacctt tcagtgaatc cagcgttggc
    39481 tgaatcggtg aacctttggc agcaatcaaa cgagaatctg cggcgtacag cttgtcggag
    39541 aaggcaatct cctgctgacg tttatcggta atggaaagcg acgaaataat ggcgtcgatt
    39601 tttttcgctt tcagtgaggg gatcagcgcg tcaaagtcac tggcaaccca ggtacatttc
    39661 acctgcatcc gtttgcacat ctcgttaccg agatcgatat caaagccaac aaaatcacct
    39721 ttagcatctt tcgacgagaa cggcgcatag gtggtatcgg ttccgatacg taccgtctcc
    39781 ggtagcgccg catagctgga agccgctgcg gagagaccga ctaacaaaga cagagcgaga
    39841 atcgacttct tcatacataa ccctcaagtg aaaatggctt ttttatgttg tgtattgtgt
    39901 tgtgtgtttg caggctcttt catgcaggtc ttatgccatc ttgacgacaa cagtaacatt
    39961 caacgttaaa tatgttaata agacgttgca ttattgtcct gaagttgaag atagcaggta
    40021 tagcggttga atcgcagcgt ttcgattgaa tggcagaaac aaaatgtcga ggatttgatc
    40081 gcggttgcaa aattgccctg aaacagggca acagcggagt tatgcgccct gccagcgggc
    40141 aaagagatct tcaggaaggg ttattgcaaa ctggtcaaga acacgattaa ccgtctgatt
    40201 tatcacatca tcaagggatt gcgggcgatg ataaaacgcc ggaacgggag gcataatcac
    40261 cgcaccgatt tctgccgcct gagtcattaa acgcagatgg cctaagtgca atggtgtttc
    40321 acgcacgcag agcaccaacg ggcgacgctc tttcagcacc acatctgccg cacgggtcag
    40381 taagccatca gtatagctat ggacaatgcc ggaaagggtt ttgattgaac agggtaaaat
    40441 caccatcccc agcgtctgga aagaaccgga agagatgctg gcggaaatat cgcgcgcatc
    40501 gtgcgtgaca tcagctaatg cctgcacttc gcgcagggaa aaatccgttt cgagggataa
    40561 ggtctggcgc gccgcctggc tcatcaccag atgcgtttcg atatctgtga catcgcgcag
    40621 aacctgtaat aagcgcacgc cataaatcgc gccgctggca ccgctgatgc ctacaatgag
    40681 tcgtttcata aatagttgcc ctgtcagact tgcaggcaga ctttgcagta tttcggcggg
    40741 agttgcaagt cagggtgcca gaccggcacc ctcagcgaag gcatcatcct tcgttatgca
    40801 tttcgagatt ttccacttcg ttctgacgtt gcactgcttt ggcgtcatca ttacgtaacg
    40861 tatcgaggaa atcgaggtag ccctgatcaa catctttggt gacgtagacg ccgttgaaca
    40921 ccgagcattc aaactgctgg atatccggat tttcagcgcg aacggcgtcg atcagatcgt
    40981 tcagatcctg gaaaatcaac ccgtcagcac cgatgatctg gcgaatttca tcaacttcgc
    41041 gaccgtgagc gatcagttcc gtggcgctcg gcatatcaat accataaacg ttcgggaagc
    41101 gaatttccgg tgccgcagaa gcgaggtaca ctttcttcgc tccggcttcg cgtgccatct
    41161 cgataatctg ctcagaagtg gtgccacgga cgatggagtc gtcgaccagc aggacgtttt
    41221 tatcgcggaa ctcggcgcgg ttggcattca gtttacggcg cacggactta cgacgcagct
    41281 gctggcccgg catgataaag gtgcggccaa catagcggtt tttaacgaag ccctggcggt
    41341 acggtttgcc cagaatacga gcaatttcca gcgcgatatc acacgaggtt tctgggatcg
    41401 ggatcaccac gtcgatatcc agatcttccc attcgcgggc aattttctcg cccagtttcg
    41461 tgcccatatt cacacgcgcg ctgtaaacgg aaattttgtc gatgaacgag tccgggcgag
    41521 caaagtatac atactcaaac aggcacggat tgctgaccgg attgtcagca cattgacggg
    41581 taaacaactg cccttcttca gtgatgtaaa tcgcttcgcc cggcgcgacg tcacgcagga
    41641 aatcaaagcc cagcgtatcg agcgctacgc tttcggaagc gaccatatat tctgtacggt
    41701 tctcgtcaat atcacgtttt cccagtacca gcggacgaat cccgtttgga tcgcggaaag
    41761 caaccatacc gtggccgata atcatcgcca cacaggcata tgcgccgcgg attaagcggt
    41821 ttgtggcagc aatggcagcg aaaatattgt cggcttccag cgggtagtgg cggaagttgt
    41881 ccagctcgct agcgaagata ttaagcagaa tttccgagtc ggaagtggtg ttgatgtggc
    41941 ggcgtttttc ttcaaacagt tttttacgca actcgtgagc gttggtcaga ttgccgttgt
    42001 gggcaagcgt aatgccatac ggggagttaa cgtaaaacgg ctgcgcttca gaggcgctgg
    42061 agctgccagc cgtagggtaa cgcacatgac caatgcccat attgccctgc aaacgctgca
    42121 tatggcgagc ttcaaataca tcgctcacca gcccgttcgc tttacgcaaa cggaagcagt
    42181 tattggcatc tatggtgatg atgccggcgg catcctgacc gcgatgctga agcaccgtta
    42241 aggcatcata aatcgactgg ttaaccggca taacaccggc gataccgaca ataccgcaca
    42301 tacgtctttt tcctcgttaa gccacatctc agagcactta cgctctgggc aagaaacttg
    42361 acgagctttg cagataatca aaaaaccatc tgatgataaa actgaactgc gggatcagct
    42421 gtgatttgct ccagtcttcg cttttcgaca ccccggtaaa ggagtcgaga aagaagagaa
    42481 tggcggcaac aatcaacaca ccgcgcaacg caccgaaaca gacgcccagc acccgatcgg
    42541 tgcctgacaa ccccgttttc tccaccaact ggcctatcac gaagttcacg atagcaccaa
    42601 caatcagggt agcgataaac agtaccgcga tggcaattcc atttcgaacc agttcgtctt
    42661 caaagcccgt aaaccagact gacaggtaag tgtagtaatg actggcaaca aagaaagcac
    42721 aaccccatgt caccagcgat aacgcttcac gaacaaagcc gcggatcagg ctaaccagag
    42781 aggaaaaagc aatcaccgcg attatggcgt aatcaatcca gaccatatgt gtcccacgat
    42841 tttacgccct gtcatcctgt tcggggcgca ttctaacaga aaaagaaaac gtttgcgtag
    42901 ggatttcctt cccacgcatc aataaaaatg gcgctgaaaa aatattcaac gccatcgact
    42961 ttttatgcct ttgcggcatc gtgcaatgcg tgtcggatgc ggcgtaaacg ccttatccga
    43021 cctacggttc taacactgcc caggcctgat aagacgcgcc agcgtcgcat caggcaagac
    43081 tgtattaatt cggcgtatag cccattacca cgccacttaa gccagaaagt tgcttcaact
    43141 cacccagcga acctttcagc ttatctttcg aggcatccgg cccaaccaga atacgggtaa
    43201 ttttaccctg cactggcgtg gatggcgacg tataaacccg ataaccggca ccgcgcagct
    43261 taccgacaat ctcattcact ttatcggcat ttttcagcgc acccagttgc acaacatagg
    43321 ctttacccgt tggcgctgct ttttcttcca cgaccggctt cggttctggc ttcggcgcag
    43381 gtggtgcttc aaccttcggt ttaggcggct ccaccggttt cggcttcggt ggggcgaccg
    43441 gtgcaggttc cggttcaaac tcggtgttat tggctgcaat agtggccgga tcgagcgacg
    43501 gtgctgcggc atcacctgcc cgcacctctt ccgctgcgcc ttccggcggc tgcgtcggca
    43561 acgcctgggt ggcggctggc atcatatcag gctcatcgcg atcgcccgct tttggaacca
    43621 gcgggatagc cgcaaactca tcctgataat gttttttctg cccgtccagc agccctggaa
    43681 gtacaatcac ccccagcgcc accagcacga tcgtgcccac taaccgattc tgaaacttac
    43741 ttgccaccgc ttctcctcgc gtcaatcact tccatgacat gtgcgaccgt gtggaaagaa
    43801 ccacacacca gcacggtgtc ttccgcttta gcgtccgcca ttgcggcatc ccatgcctgc
    43861 gcaacgctat caaatgattt gccgttaccc aaatgctcaa gcagttgttc tgccgtggca
    43921 ccgcgcggcc cttccagtgg cgcacaatac cagtcatcaa ccacgctttt caaccaggcc
    43981 agagttccgg caatatcttt atcatgtagc ataccgataa ccgccagcac gcgcccgttt
    44041 ttcggtagcg ctttcatacg cccggtgaga tattccgccg catgtggatt atgcgcgaca
    44101 tcaaaaataa cgcgtggcga ctcgctcaca atctggaaac gtcccggcaa aattgcgctg
    44161 gcaatcccgt cgcgaatggc attttcactg acttccagcc cgctggcacg cagtgccgcc
    44221 agcgctgttg cggcattcgg ttgcgggaca agcggcaacg gcagattttc cagcgtgccg
    44281 tgagcatcgc taaacgccca gtcatgatcg gtgacggaat agttccactc aacgccccga
    44341 cgttgtaaca gtgcaccttt ttcctgcgcc acatcagcaa tggtagaagg catttccggc
    44401 tcaccgacaa ttgccggttt tgcgctgcga aagatgcctg ctttctcgcg accaatactt
    44461 tcgcgatctg gtcccagcca gtcggtatga tccagcgcaa tgctggttac tacagccaca
    44521 tcggcatcga caatattggt tgcgtccaga cgaccgccca gccctacttc cagaatcact
    44581 acgtcaagtt gtgcctgttt gaacagccac aacgccgaca gcgtaccgta ctcgaaatag
    44641 gtcagggaaa tatcaccgcg tgccgattca atctccgcaa aagaggcggt gtgggccgat
    44701 tccggcaatt cctgcccctg cacacgtacg cgctcggtat aacgcaccag atgcggtgaa
    44761 ctgtagacgc ccactttgta ccctgccgcc atcagaatcg actccagcgt acggcaggtg
    44821 gtgcctttgc cattcgtacc cgcaacggta aacacaaatg gcgctggttt caggacgcca
    44881 agacgcgccg cgacctggct cacgcgctca aggccgagat cgatagtttt actgtgcagg
    44941 ttttccagat aagaaagcca cgaagccaga ggcgacgcgg cttgaggagt gcgtttgata
    45001 atcatggtat ccgctgattc gttacggtga gaatagcaaa agggcagagc cagtggccct
    45061 gcccttatca gttatcaggc ctcaggttcc tgatccggta ccgggggtac cactacgcct
    45121 tcacgcggcg cttcaggatt cggcgctggc agattcatca acttcgccag aatgctcgcc
    45181 agtttcaggc gcatttccgg acgatggacg atcatgtcga tggcgccttt ctcgatcagg
    45241 aattcactgc gctggaatcc tggcggcagt ttttcgcgaa cggtctgttc gataacacgc
    45301 ggaccggcaa agccaattaa cgctttcggt tcagcgatgt tgagatcgcc cagcatggcg
    45361 aaacttgcag aaacaccgcc catcgtcggg tcggtcagca cggagatgta cggcaagccg
    45421 cgctcctgca tttttgccag tgccgcagag gttttcgcca tctgcatcag cgacatcagt
    45481 gcttcctgca tacgtgcgcc accagaggcg gagaagcaga tcagcgggca gttatcttcc
    45541 agcgcctgct caacggcacg cacgaaacgt gcacccacaa cagaccccat tgaaccgccc
    45601 ataaaggcga actcgaatgc cgcagcgaca accggcattc catacagagt gcctttcatc
    45661 accaccagcg catctttttc gccggtttct ttctgcgcag aagccagacg gtctttatac
    45721 ttcttggagt cacgaaactt cagcacatct ttcggctcaa gctcgctacc cagctccaca
    45781 aggcttcctt catctaacag gctatgcagg cgattacgcg ctgtcatacg catgtgatgg
    45841 tcacacttcg gacagacctc aagattacgt tccagctcag cgcggtataa aacctgaccg
    45901 cagctatcac acttagtcca caccccttca ggaatgctcg ccttgcgggt gggagtaatg
    45961 ttgcttttaa ttcgttcaat ccagctcatt agggaccttt ctgtctgaac ctggttcgat
    46021 gccagtttta tctttgggga cgcataatgc catttttgcc cccaacagac catgaatgtt
    46081 gcacattaaa acataacagc ccgaaacttt ggataaaaaa gtggtcgaac cgcggagtta
    46141 ctttttattt tgcggcgcgt gccgcagcgc gtttgtgacg aataaattcg aaaaccccag
    46201 gcaatattga aacaaaaata atgccaacaa tcatcagctt aaggttgctc tgaatgaagg
    46261 ggagcgtgcc gaagaaataa cccgcgtagg taaaaatcag tacccacaac agtgcgccga
    46321 tcacgttata agcggcgaaa tgacggtacg acatgtggcc cattcccgca acaaacggcg
    46381 cgaacgttct gacgatcggt acaaaacggg cgagaataat cgttttgccg ccatgtctct
    46441 cgtaaaactg atgagtctta tcaagataac tgcgacgaaa gattttcgaa tccgggttac
    46501 tgaacagttt gtcaccaaat aaccgtccaa tggtatagtt tactgcatca ccgacaatag
    46561 cggcaatcaa cataagcacc accatcatat ggacattgag atcgttagtt tccagcgacg
    46621 ccaatgcacc agcaacaaac agcagcgaat caccaggcag gaatggcgtt accaccagcc
    46681 ccgtttcgca gaacagaatc aaaaacagaa tggcataaac ccagacgccg tactccgcga
    46741 ccagctctgc cagatgcacg tcaatgtgca ggataaaatc gatgaggaaa taaatcaggt
    46801 ccatatttgc ctatgccttg tactcgtcat ctttcaggct gtaactacgt tggctacagc
    46861 ctgaaatgtt ccgagcatta ttctcgttag tccgccagaa atagcgggcc cattggcggt
    46921 tttggaagat cataccggtc agggtaatcc accgcgacca gatacagccc ttccgctttt
    46981 gccgttgctg ccgccagcgt tctgtccttt gccgccagca actctgctat ccatctctcc
    47041 ggctggttgt gggcaccgac ttccatcagg ctgccgacaa tattcctgac catatgatgt
    47101 acaaaggcat tcgctttgat atctaccacc acataaggac cgtgacgcgt gacattaata
    47161 tgcataacgt tgcgccacgg ggttcgggac tggcactgca ccgcacggaa cgaggtgaaa
    47221 tcattctcgc ccagcaagca ttgcgcagcc cgatgcatcc gttcagcgtc cagcggttcg
    47281 taaaaatggg ttaccccttt actcaatacc gccgggcgca gccgatgatt gtagatgatg
    47341 tagcgataac ggcgagccgt ggcgctaaat cgggcatgaa aatcatcagg tacagcttta
    47401 acccaacgca cggcgatgtc accaggtaaa ttcgcattta cgcccaaggt ccacgccgcg
    47461 tctttgcgct gcgcggttgt ttcgaaatgc accacctgcc cggtaccgtg tacccctgcg
    47521 tcagtacgcc cggcgcagaa gacggtgatg ggttcgttcg ccacctggga gagcgccttt
    47581 tccagcttct cctgcacact gcggacttca ttctgccgtt gccagccgta atacttactg
    47641 ccgtcgtact caatgcccag cgcaatttta taaactggca gttgttgctg gtcggacatt
    47701 agtacagata ctcctgcacc agtttctcgg cgatttttac tgccatcagc gcgccgccaa
    47761 agcgaacgtt atcggccacc gaccagaact ggacttgctc cggcataccg tagtcattac
    47821 gcacgcagcc aacagaaaga tgcggcgtac ccgaagcatc ccctacctga gtcgggaatt
    47881 cgttctcttc agagagcaca atatcttcgc cctgagcaaa cgcatcacgc gcttcttctg
    47941 ccgccagcgg acgcagtgct tcaaagttga ccatctgggc atgaccgtag aataccggtg
    48001 cctggacgac gcttgccgaa atcatcagcc cttcgtcctg caggattttg cgtacttcgt
    48061 caacgatacg acgttcttca cgcacgctac cttcgctatc cggcaacaac ggcagcatat
    48121 tgaacgccag ttgacgaccg aagaaatctt cttcgtcaat cggaatgccg ttgagcaatt
    48181 tcgcactctg ccccgctaac gcatcgaccg cttttttgcc ctgggcggag gctgaaatca
    48241 ggctggtaac actgatacgc gacaaaccgc cctgatcgat taacggtttc agtgccgcca
    48301 gcagctggct ggtcagactg tctggtacgg cgatgacatt ccggttgcgg taatcggtca
    48361 gtacaaacgg gtttacttcc ggcaccacca gcggtacgtc gggttcgaga gcaaacagtc
    48421 cgctgctgtc gatcaccagg caacctgagt tggtcgcttc ttcaacccag gcagcggtag
    48481 cttctttgcc tgcgacaaaa aatgccagct gcgcctgcgt ccagtcgaat tcagcggcat
    48541 cctgcacagt gattgtttta ccaccaaagc gcagttgttc gcctgcgctt tcgttacgtg
    48601 ccagtgcata aatttcccca accgggaact gacgttcagc cagcgtttca agcagggctt
    48661 cgcccacagc gccagttgcg cccaggacgg caatgttcca gccttcagac atggtggttt
    48721 actccagaaa tagcaaagct ccctgccaat gttacagcag agagcatgaa gaagagatta
    48781 acgtgccgga tgatgaacgg cgttaaaacc cagtttacac agcaatgatg ccgcactggc
    48841 gtcatcacaa attacataca gagatgacca ttcacggcgc tcaagatagt ttttgcgcag
    48901 tttatcgaac tcacccggta tcccggcgac tttacgcagc ggtgcgtcat cgcggcgcac
    48961 atcatacacc aaatgcacca gccttttcag cgtcggttga tcgagcgggc catgcagcgt
    49021 aatgcgacca aactctggcg cgggcagtaa tgtatccagc gcaacgtgct gttcatgccc
    49081 aataaacttg ctataggctt caaacacttg cgtagtaccg cgtgctttac cttccagggt
    49141 ataacctgcg atatgcggcg tgccgatatc cacttttgtc agcaactcca cgttaagttc
    49201 cggttcgcct tcccagacat ccagcactac gcttaacttc tggccttcat tcaggcaggt
    49261 cagcaacgca gtattatcga cgactgcgcc acggcaggcg ttaatcagaa tcgctcccgg
    49321 cttcaggcta cggatcagtt tttcatccgc cagatgcagc gttttgtacg gcccatcttt
    49381 gaagagtggc gtatggaaag tcagaatatc cgcatgctga actaattcat ccagcgagcg
    49441 gaaatcaccc tcatccccac ggtcagcgcg aggcggatcg caaagcaatg ttttaatccc
    49501 taacgcttcc agtcgcgcct gcaatcgacg tccaacgtta ccaacgccca cgatccccac
    49561 cgtacggtcg tgcagtgaaa atccatcgcg ttcggcaagc atcagtagtg aggagaaaac
    49621 atattccacc accgcaatcg cattacagcc aggtgcagcg gaaaaaccaa ttcccgcctg
    49681 cttcaaccat gcttcatcga catggtcggt ccccgcagtg gcagtgccaa caaatttaat
    49741 gggttttcct gccagcaaag attcattcac tttcgtgacc gaacgcacca tcagcgcatc
    49801 cgcgtctgcc agttgagcga cggggattgg acgcccggga accgcggtca cctcacccaa
    49861 acggctaaat aagtcgcggg cataaggcat attttcatca acaaggattt tcacgtttgt
    49921 gttacctgta tgagacgaga gttaaccgga caagtgtgcc ataatctcgc ggccaggcat
    49981 acttgcgaag atttcaggta taaggatacg taatgataca acctatttcc ggccctcctc
    50041 ctgggcaacc accaggtcag ggagataacc tgccgtctgg cgcgggcaat cagcctttat
    50101 ccagtcagca acgtacttcg ctggaaagct taatgacgaa agtgacctca ctgacgcaac
    50161 aacaaagagc agaactgtgg gcgggtatca ggcacgatat tggtctgtcg ggagattcac
    50221 cgctgctttc gcgtcacttc cctgccgctg agcataatct ggcgcaacgt ctgctggccg
    50281 cgcaaaaaag ccattctgcc cgccagcttt tagcgcaatt aggggagtat ttacgtctgg
    50341 ggaataatcg tcaggcggtc acggattata tccgtcataa ctttggtcag acgccgctga
    50401 atcagctctc accggagcaa ttaaaaacca ttctcaccct gttgcaggaa gggaagatgg
    50461 ttattccgca accacagcag cgcgaggcga ccgaccgtcc tttattaccg gcggagcaca
    50521 atgcgctaaa acagctggtg accaaacttg cggcggcaac gggggaaccc agcaaacaga
    50581 tctggcaatc gatgctggaa ctttccgggg tgaacgatgg cgagttaatt ccagcgaaac
    50641 tgtttaacca tctggtgacc tggttgcagg cgcgccagac gttaagccag caaaatacgc
    50701 cgacgctgga atcactacag atggcgctaa aacaaccttt agatgccagt gaactggcag
    50761 cgttatcggc gtatatccag caaaaatatg gtctttctgc gcaatcatcg ctttcttctg
    50821 cccaggccga ggatattctt aatcagcttt atcaacggcg ggttaaaggg attgatccgc
    50881 gtgatatgca accgctgctt aatccttttc caccgatgat ggacacgttg caaaatatgg
    50941 caacgcgtcc cgcgctgtgg atactgttag tcgcgattat cctgatgctg gtctggctgg
    51001 ttcgttagcc ccgacgaaat gacagtatcg tgacaataat tcccagcacc gcagagatcg
    51061 ccccggcaag aaataccgaa gagtaaccaa acgtggtcgc cagcattccc gccagcggcc
    51121 cggagacgcc gagggcgata tcctgaaacg cggcgtaacc gcccagtgcg gtgccgcgaa
    51181 cttgtgaggg gacgcgttta accacctcca cgcccagcgc aggaaagata agcgaacatc
    51241 cggctccggt taacgccgcg cccgctaatg cgacccacgc acctggggct tgccagagca
    51301 gcaacaagcc caccgtttct acaagcagag agacaatcgc cactttcacg ccgccaaaac
    51361 ggtccggcat ccagccaaac atgacgcgca tcacgacaaa tgcgccgcca aactcggtaa
    51421 gagtaaagcc cgccatcgcc catcctttgc tggcaaagta gagcgaaacg aaagtcccga
    51481 taaccgcaaa accaacgcct tgtagtgcca gccctaaccc tggtttccag ataaacccga
    51541 caacgctcca cagcgatgga cgttctcccg ccagggccgg tactttgcgc actgtgccgt
    51601 tacaggccca cgccagtaag ggtaatacca ttgtggtgat cgccagtgcg gcaaaaccgt
    51661 aatggctatg aatcaacagg ccaagcggag caccaacagc gagggcaccg taaatcgcca
    51721 ttccattcca tgacatcact ttgccagagt gttttggccc tacgatgcct aacccccagg
    51781 tcagagcgcc tgtcagtaac tggctttcac caaacccaag aatcaaacgc ccgatgacca
    51841 acagggcaaa tttgaacggt gcggagacag gcaaaatcgc cgccagcagc aacgcgccgc
    51901 cagccagacc acaagctaac atcccctgaa gcgccgaacg ttttgcgcca tattgatcgg
    51961 ccagtcgccc ggcatagcca cgcgtcagca ccgtagccag aaactgaatc ccgacggcaa
    52021 tgccgaccat ggtattgcca tagcccagtt catgatgaac aaacagcggg ataaccggca
    52081 acggcagccc tacggtcatg taggtgagaa aaaccgcaaa agcgatgcgg aagagcgaaa
    52141 aattggcaga agatcgtgtt tcggtttggc ttacagcagt catgcattac tccagaatgc
    52201 agcgcaaggc gaggagtatc cccgtctcat ctctctggtt tcagggttac ggtgcgttgg
    52261 caggatttaa cgcgtacgtc ttttcagaag gaaatcgaca aagcgggaag tttgcctgga
    52321 actggtggcg attgtcaatg atgtgaaaaa gggaaccatc aggttccctt ttgcgttagt
    52381 gccggaggcc gcattggcgc gtaacgtcgg atgcgacgct ggcgcgtctt atccgaccta
    52441 ctccggccta ctgcgaatta atctttcagc ttgcgcatta ccagcgtggc gttggtgccg
    52501 ccgaagccga agctgttaga cataacagtg gtcagttcgc gatcggtcgt ttcggtcacg
    52561 atgttcagac ccgcagcctg ctcgtccagc tcttcaatgt tgatgctcgg ggcgataaag
    52621 ccgtgttcca gcatcagcag agagtagata gcttcctgca cacccgcagc gcccagtgag
    52681 tgaccggtca tggctttggt tgcagaaatc gccgggctct tatcgccgaa cacttcacgg
    52741 atagctgcca gctctttcac gtcgccaacc ggagtcgaag taccgtgaga gttcaggtaa
    52801 tcgattgggg tatcaacgcc atgcatcgcc atctgcatac aacgtactgc gccttcgcca
    52861 gacggagcaa ccatgtctgc accatcagag gttgcgccgt agccaacgat ttcagcatag
    52921 atgtgagcac cacgcgccag cgcgtgttcc agctcttcaa ccactaccat accgccgccg
    52981 ccagcgataa cgaaaccgtc acggtgagcg tcgtaagtac gggaggcttt ttccggggtg
    53041 tcgttgtatt tagtagacag cgcacccatc gcgtcgaatt cgcaggccat ttcccagcac
    53101 agctcttcgc cgccgccagc aaacacgatg tcctgtttgc ccagttggat ctgctctact
    53161 gcgttaccga tacagtgtgc ggaagtcgca cacgcggagc tgatggagta gttaacgcca
    53221 tgaattttaa acggcgtggc gaggcaggca gaaacgccgg atgccatcgc tttggtgacc
    53281 acatacgggc caaccgcttt caggccgcgc gggccgcgca ttgcgtcagc gccgaatacc
    53341 tggaaacgcg gggaaccgcc gccagaacct gcaatcaggc caacgcgcgg gttattctgg
    53401 taagcttccg gagaaaggcc cgcatctgcg attgcctgct ccatagaaag gaatgcataa
    53461 atggatgcgt cgctcataaa gcgcacaact ttgcggtcaa tgaggccagt ggtatccagt
    53521 tttacgttgc cccagacgtg gctacgcatg ccggaatcct tcagctcctg agagaaagtg
    53581 atccctgaac gtccttcacg cagagatgcc aggacttcct gctggttatt accgatgctg
    53641 gaaacaatgc ccaggccagt aatcactgca cgtttcattc aatacctctg taagtcgcac
    53701 atagagtaag tttcgaatgc acaatagcgt acacttgtac gccgaacaag tccgatcagc
    53761 catttaatag agaaatttgc gcagccttac acacatcgct aagatcgagc caccgcctgt
    53821 aagacgagta acttacgtga aacactactc catacaacct gccaacctcg aatttaatgc
    53881 tgagggtaca cctgtttccc gagattttga cgatgtctat ttttccaacg ataacgggct
    53941 ggaagagacg cgttatgttt ttctgggagg caaccaatta gaggtacgct ttcctgagca
    54001 tccacatcct ctgtttgtgg tagcagagag cggcttcggc accggattaa acttcctgac
    54061 gctatggcag gcatttgatc agtttcgcga agcgcatccg caagcgcaat tacaacgctt
    54121 acatttcatt agttttgaga aatttcccct cacccgtgcg gatttagcct tagcgcatca
    54181 acactggccg gaactggctc cgtgggcaga acaacttcag gcgcagtggc caatgccctt
    54241 gcccggttgc catcgtttat tgctcaatga aggccgcgtg acgctggatt tatggtttgg
    54301 cgatattaac gaactgacca gccaactgga cgattcgcta aatcaaaaag tagatgcctg
    54361 gtttctggac ggctttgcgc cagctaaaaa cccggatatg tggacgcaaa atctgtttaa
    54421 cgccatggca aggttggcgc gtccaggcgg cacgctggcg acatttacgt ctgccggttt
    54481 tgtccgccgc ggtttgcagg aagccggatt cacgatgcaa aaacgtaagg gctttgggcg
    54541 caaacgggaa atgctttgtg gggtgatgga acagacatta ccactcccct gctccacgcc
    54601 gtggtttaat cgtacgggca gcagcaaacg ggaagtagcg attatcggcg gtggtattgc
    54661 cagcgcgttg ttgtcgctgg cgttattacg gcgcggctgg caggtagcgc tttattgcgc
    54721 ggatgaagcc cccgcgctgg gtgcttccgg caatcgccag ggggcgctgt atccgttatt
    54781 aagcaaacac gatgaggcgc taaaccgctt tttctctaat gcgtttactt ttgctcgtcg
    54841 gttttacgac caattacccg ttaaatttga tcatgactgg tgcggcgtca cgcagttagg
    54901 ctgggatgag aaaagccagc ataaaatcgc acagatgttg tcaatggatt tacccgcaga
    54961 actggctgta gccgttgagg caaatgcggt tgaacaaatt acgggcgttg cgacaaattg
    55021 cagcggcatt acttatccgc aaggtggttg gctgtgccca gcagaactga cccgtaatgt
    55081 gctggaactg gcgcaacagc agggtttgca gatttattat caatatcagt tacagaattt
    55141 atcccgtaag gatgactgtt ggttgttgaa ttttgcagga gatcagcaag caacacacag
    55201 cgtagtggta ctggcgaacg ggcatcaaat cagccgtttc agccaaacgt cgactctccc
    55261 ggtgtattcg gttgccgggc aggtcagcca tattccgaca acgccggaac tggcagagct
    55321 gaagcaggtg ctgtgctatg acggttatct cacgccacaa aatccggcga atcaacatca
    55381 ttgtattggt gccagttatc atcgcggcag cgaagatacg gcgtacagtg aggacgatca
    55441 gcagcagaat cgccagcggt tgattgattg tttcccgcag gcacagtggg caaaagaggt
    55501 tgatgtcagt gataaagagg cgcgctgcgg tgtgcgttgt gccacccgcg atcatctgcc
    55561 aatggtaggc aatgttcccg attatgaggc aacactcgtg gaatatgcgt cgttggcgga
    55621 gcagaaagat gaggcggtaa gcgcgccggt ttttgacgat ctctttatgt ttgcggcttt
    55681 aggttctcgc ggtttgtgtt ctgccccgct gtgtgccgag attctggcgg cgcagatgag
    55741 cgacgaaccg attccgatgg atgccagtac gctgacggcg ttaaacccga atcggttatg
    55801 ggtgcggaaa ttgttgaagg gtaaagcggt taaggcgggg taatctgctc tcgcagtgtt
    55861 tgccggatgc ggcgtgaacg ccttatccga cctacgtgtg acgtgtaggc ctgataagac
    55921 gcgataagcg tcgcatcagg catggtgctc cagacgccgc aacttactgc tgtgacgcct
    55981 gctgaaacag gttttcccac atatcggtca ccagcgcctg gtcacgcggc gacaattcac
    56041 cggcaccaat ggctttttcc agactctggc taacggtcgt atgcaccgcc tgagcggagt
    56101 ggtcatcacc actttccagt tctgcgatgg ctaatgtcag gtggccacgc aaatacccac
    56161 tggcaaacaa ctcatcatca ctggcatggt caaccatgcc gtcgattaat gccagaatgc
    56221 gtgattcaaa ctccgcgatc atcttctttc ctcattgtaa aacgtggcac cggcttcagt
    56281 tgagcgcttc cggccacggg aactgttccg ccgtaagttc cggcgtgtga taataatttt
    56341 gtagtgcttt aatgaagcgt gccggacgct cgggaatgcc atttgtcaga taatccatca
    56401 cctgcgcatg tacccgccgt tgaaacacaa cacgatccgg ttcgaagtcg ccttccagat
    56461 tgtcgcaact gacgttaaac ggatatcccg ccgccacaca gaacaaccag tcgagtgcct
    56521 gcggcttcac ttcaacatct tcaaactggc tttgcgtttg ggcatcacgc ccatccgggc
    56581 aataccagta gccgaaatca accagctcac ggcgcgcttt tccggcaata caccagtgcg
    56641 aaatctcatg aattgcgctg gcataaaagc cgtgggcaaa gacaattcgg ttatacggta
    56701 cttccgcatc agcaggaaga tagatcggtt cgtcgtcgcc tttaatcaga cgggtattaa
    56761 attcatcggc aaagcagcta ttaaaaattt caatcaactg ctcatagtgg tgtgtactgt
    56821 tcattagttc atccccaacc agtggaggat ctcctgtccg tggctatcgt aaagtaattt
    56881 ggcactcatc accgccgaga cgataacaat catcgggcgg atcagctttt gtcctttgct
    56941 caacaccagc cgtgaaccca tgcgcgcgcc gaggaactgc cccaccagca tgacaaagcc
    57001 cgtcgcccaa atcactttgc cgccgagaat aaacagcagc agaccgccaa tgtttgacgt
    57061 tgcgttgagt aatttggcgt gagccgtggc tttggcgagg ttgaatccgc acagcgtaac
    57121 gaaggccagc gcgtaaaacg atccggcagc cgggccaaag aatccgtcat aaaaaccgac
    57181 gcagccaccg gcaattaacg cgaagggtaa accgtacatc cggcgctggc gatcttcttc
    57241 acccagcttt ggcatcagca agaaatagag gccgatacaa atcaccagaa tgggcaaaat
    57301 ctgccgtaag acatcagcct gaacgtactg taccaacagt gcgccgctca ttgagccgac
    57361 aaaggtcatg gcaatattga gtttctgatc gcttaaacta accactttgc ggcgaataaa
    57421 gtagatagtg gcggaaatag agccgccgca ggcttgcagt ttattggttg ccagcgcgtt
    57481 agcgggagac attcccgcag ccatcaatgc cggaatggtg agtaacccac cgccaccggc
    57541 aatcgagtcg ataaatcccg ccagcatggc gacaaaaaag agaactccca gcaacagcgg
    57601 ggaaaccata aacaggctat taaacgtttc cattagatca cgtgctcatc cagtagcgcc
    57661 tggcaggaag gcggcaacgg aggcggtgtc ttcttctcag gctttgttgt tcccggtttt
    57721 agaggtgcaa accagctttg cagttctgcc ccgcaaccat cgcctgatgg cggtaaaggt
    57781 tgatcttcac actccagact atcggcaggg caacgtaatc gtacatgcat atgcgcgcga
    57841 tgctggaacc agggtcgcac tttgcgcaac cagtcgcgat cggtgcccgc atcaaggcaa
    57901 agttgttgtt taatcgccgg attaacaaaa atgcgcgtga cgtctttgtc ctgggcagcg
    57961 agtttgatca agctgaaaat ttctggcttc cacagcgtgg agacaacgtg tttaccgtcg
    58021 cgggaaacta agtccagtgc ttgcgggcgc aagagctgcg cggaggtcca gcgagttttc
    58081 ggcagttgca gaaagatatc gacatccagt ccggtctggt ggctggcatg accgccgttg
    58141 aaacgcccac cagcgggcat ccccatatcg ccaatcagca ccgtacccat gcccagattg
    58201 ctcacctggc tactcagacg ctggataaac atcaccagat ccgggtgacc gaaatagcga
    58261 cgctgatcgg tacgcatgac ctgataatgt tcggactgta tcggcaacgt gtcagcgccg
    58321 acaatacagc cattagaaaa actgcctatc gattgtgcgc tacccggcac aggttgggtt
    58381 attttttgcc acggcgtcgc tgccaggcta gcgctactgg caagcagagc cagcagcgca
    58441 atcgcggttt tgttcatttt ttaccagcgt ggaatatcag tcttcacatc ggcattttgc
    58501 gcccgctgcc gtaacaggtg atccattaaa acgatcgcca gcatcgcttc agcgatcggc
    58561 actgcacgga tcccgacaca gggatcgtga cggcctttgg tgatcatctc aacttcttcg
    58621 ccaaagcggt taatagtgcg ccccggcacg gtaatgctgg aggtcggttt cagcgccata
    58681 tgagcaatga tttgctgccc gctgctgata ccgccgagaa tgccgcccgc atggttgctc
    58741 tggaaaccgt ctttggtgat ttcgtcgcgg ttctggctgc cgcgcagcgc cactacgtca
    58801 aaaccgtcgc caatttccac gcctttcacc gcgttgatgc tcatcagcgc atgggcgatg
    58861 tcggcatcca ggcggtcaaa gaccggctcg ccaagtccgg cggggacacc actggcaaca
    58921 acggtgactt tcgcgccgat ggagtcgccc tcttttttca gcgcgcgcat cagttcatct
    58981 aacgcgtcga ttttgtccgg gtccgggcag aagaacggat tttgttcaac cagcgaccag
    59041 tctttgattt ccagcggaat gtcgcccatc tgggtcaagc agccgcggat ttcaatacca
    59101 aatttctcgg cgagatattt tttggcaatc gcccctgccg ccacgcgcat ggcggtttcg
    59161 cgggcggaag aacgtccacc gccgcgataa tcgcgcagac cgtatttttg ttcgtaggtg
    59221 taatcagcat ggcctgggcg gaaaacgtct ttaattgcgc tgtaatcctg agagcgctgg
    59281 tcagtgtttt cgatcaacaa gccaatgctg gtgccagtag taacgccttc aaaaacaccg
    59341 gagagaattt tgacctgatc cggctcgcgg cgctgggtgg tatagcgcga tgtcccaggg
    59401 cgacgacggt cgaggtcatg ttgcaggtcc gcttccgtca gcggaatgcc tggcggaaca
    59461 ccatcgacga tgcagccgag cgccagccca tgcgattcgc cgaaggtggt tacgcgaaag
    59521 agttgtccaa ttgtgtttcc agccatcacg gctccgttat tgttgtgttt gcgtgtttac
    59581 ttaatcttta taaatcgcga aatattcacg ggccgccagc agctgctctt tggtgagcat
    59641 aaacacacca tcgccgccgt tatcaaactc cagccaggtg aacggaacat ccggatattg
    59701 ttccataaga tgtaccatgc tgttgccgac ttcacaaatc aacacgccat catcagcaag
    59761 gtaatctgcc gcgtttccaa gaatgcgacg cgtcagttta aggccgtcgg tgccagatgc
    59821 caggcccagt tccggctcgt gacggtattc gtttggcagg tcggacatat cttccgcgtc
    59881 gacatacggc gggttagtga caatcagatc gtactgcact ttcggcaaat cacggaacag
    59941 atcggaacga atcggaatga cgttgtggat cagaccgtgt tcttcgatgt tctgttcggc
    60001 aaccgccagc gcgtcgggag agatatctac cgcgtcgact tccgcttccg ggaaggcata
    60061 agcacaggca atggcgatgc agccgctacc ggtacacata tctaaaatat gctgcggttg
    60121 cttgctaatg agtccggcaa atttattgtt gatcagttca ccaatcggcg agcgcggcac
    60181 cagcacgcgt tcatcgacgt aaaattcatg accgcagaac caggctttgt tggtcaggta
    60241 agccaccgga atgcgctcgt tgacgcggcg gatcacgcgt tcaacaatac ggtgtttttc
    60301 gctggaggtc agacgcgcgg tgcgcatatc ttccgggata tccagcggca ggtagagcga
    60361 tggtaacacc agctgtacgg cttcatccca cgggttatcg gtgccgtgac cgtaccagat
    60421 atttgccgcg ctgaagcggc ttaccgacca gcgcaacatg tcctgaatgg tttgcagctc
    60481 atttactgct tcatcaacga aaattttatc cacgtattcc tccagggcat gatcgcaata
    60541 atttcggcgg ctagtttgcc atgaagatga cgataaatca gcattcacgc gcggtgagtg
    60601 aggaaaaata cgtttaaaac gatcgattgt gctacgagtc gggtaaactg taggaaaatt
    60661 agaaatagag acagataaat gaaaaagaaa acaacactta gcgaggagga ccaggctctg
    60721 tttcgccagt tgatggcggg gactcgcaag attaagcagg acacgattgt ccaccgaccg
    60781 cagcgtaaaa aaatcagcga agtgccggtg aaacgcttga tccaggagca ggctgatgcc
    60841 agccattatt tctccgatga gtttcagccg ttattaaata ccgaaggtcc ggtgaaatat
    60901 gttcgcccgg atgtcagcca ttttgaggcg aagaaactgc gccgcggcga ttattcaccg
    60961 gaattgtttt tggatttaca cggtctgacg cagctgcagg ccaagcagga actgggcgcg
    61021 ttgattgccg cctgtcgccg cgaacatgtg ttttgtgcct gcgtgatgca tgggcacggg
    61081 aagcatattt tgaagcaaca aacaccgctg tggctggcac aacatccgca tgtgatggca
    61141 tttcaccagg caccgaaaga gtacggcggt gatgcggctt tgttggtgtt gattgaagtg
    61201 gaagagtggt tgccgccgga gttgccctga atgctggcag caaatgccgg agcagcgtaa
    61261 acgccttatc cggcctacaa atatgtgata attcaataaa ttgcaattca cttcgtaggc
    61321 ctgataagac gcatcagagc aatactacga actacagttc attcaataca tactgaaaac
    61381 gacacgtcga atcaatcggt tgcccactac tggtattcac attcaccaca atatcgtgta
    61441 gtactttatc cgttaccgga tgacgagttg gcgtaaccgt taccgttgat ttatcacaac
    61501 cgtaagaggt aaaggttaac aattctgata ccgcgcggca gtatgctcct ctgccatcat
    61561 tatcagaaaa attactgcca tcacagagtc tgccgccttc catcaggtag gtcgaaagcg
    61621 tggtggtcac tgttgacccc gtggactgaa ttcgtaacgc cttattatgg ctgacataat
    61681 tttcactcca ggagatatcg tatgaagtga ccggtatcgt tgggcatatt gtgttatgca
    61741 ctgtaggggc cgtatagctt ccaaatccgg gtaaatctgt ataggaataa aaggatgcga
    61801 tccaagggca accataatca tcagatgcaa cggtaatttt aaacgtcata tatgcacaac
    61861 gaaaaatttt cgtatctatg ccagggcaat ttgagttatt aattgtatca aagtcagtca
    61921 gtaccaaagt tggagctgaa cccgacccag accaggaaat aacccgcaac gtatcgcctt
    61981 tatacgcaaa agaatcatat ttcccatatg taacctcctt tggcgttatc accgtaacat
    62041 tgaaaacact gctcgctaca gcaccggtta ccttgtccat ggcataataa actctcacac
    62101 tgtcaaaact cgcactcata agtttatctc cagcataact cttagatgcg ccgacaacac
    62161 tcagaacgag cagcattagg ctcgcgaaaa ttatggtcca ttttttcatt tcagaaatac
    62221 tcaaaggttg ccgtcactgt ggcactaaag tctccggacg ttacatctcg cccgctcttt
    62281 gcctgtaacc aggtattaaa atttaggctg ttatttccct gattcagcgg gagcgtgacg
    62341 cctgttgtat tgttgattgg caccgatgtg cctgccgcag tttcaatacc aatcccagcg
    62401 ccggatgctg atgacgagga gtcaaaggcc agaaagccgg gtagagcact atcttctgtt
    62461 cctgtcaacg ttactttgac attaaacaat gtagaactat gacaatcttt caattgaaac
    62521 accaatggca ctcgctctga ctgcccaagg ttcattaaat cgctggcggc aatggcagga
    62581 aaatgtactt ctgccaactg gctaccatta atcactggcg tacacgattt actgattaaa
    62641 ttgccggtaa agtgcaaatt atctcctgct attgtggttc cactgcccag caacatcagg
    62701 ctactaataa aaaacaacgt acgctttttc ttcattggta atctacctgt aacgtggccc
    62761 aggcctcaaa gtccgcctct ggcagaacga caccactctt ttttactggc acagccgtta
    62821 acaccggtaa atcggtttca ttcaccataa gtggcgtatt gatcgtaaaa ctctgccctg
    62881 cctgtttgag ctgaatgcct aaaccagtga tattgctgct tactgcagca ttatcaaaat
    62941 cgcttagtgt gccactccag ctcaaggtca tttccattaa tgggtcgcga accgccgtat
    63001 cgcaggtaat ggaatacggc acggtttgct cataattgct gccattaatg tcatcaataa
    63061 gcacatcctg aaaagttatt tcaatggttt gagcgttgct aatcgtgcag ttaggcggct
    63121 gcactaaata accgtgaaaa gagacgtttg agagtgccat cgagcaaaag ccccacagca
    63181 aaataagcga gatccgtttc attggtaatc caccacaatt gtggcactgg cgttaaattc
    63241 agctgccgct aacgttgtac cactctgttt caccggcaca gcttccagaa cgggaactcc
    63301 cgaggaaaaa ttaaatggca accatgagcc agaggttaag tcgagtattg tgtgatcact
    63361 acttttctga acccgaatac caaacccagg aatacccgtg ctgataactg tttcaccgtt
    63421 aatcacgacc gttgtcgcct gcatctgtag acgcaggtca tccattgcca gattgttaca
    63481 aacaagcgag tagtcaatgg gacgtttata atcaacccca ttgatcttat taatgaatac
    63541 attaccaaac tcaactgccg caccatttac cgtacaagga ggaggggcat cgacaagcac
    63601 ccgtaaattc atacttactg acttactcgc tgcaactgca aagccgctac caacacaaca
    63661 tatcagtaac ccagttaccg cccagacaga acaagatcct ttcatactgg ctctcccggc
    63721 ctcagccttt gcggctctgc tcttcatcga cctggcaact gttaccgttg caagtgaaaa
    63781 agagcggcat ccgagcgcca tagtcgttga cataggtcag taccggcacg ctacccatct
    63841 tcacattcag cggtgcagag gttttcggcg acatcactat tggcgagaaa ccggctgcag
    63901 gattgccatt tttctgtgtg ctggcattac tgaaaatgac gtaatacggc gtcgggttat
    63961 tgacggtaaa ttcctgaccg ttgcgggtta aagtaacctt atattgccag gggtttttca
    64021 tgctgacgtt ctccagcgcc ttcggtcgcc agaacaactt aatacgcgtt tgtagtgcta
    64081 tttgcagcgt gttggctttg tttgattttg gcgggatctc acgcacgttg aagtagaaca
    64141 ggctttcacg atcggcaggc aatttgttga tatccggcat tccctgcact tttacctgcc
    64201 cgttcatcat tgaatcgatg cgttgcaccg gtggcagaac agttaacggt gaactgatct
    64261 tgttaccctt ttcatcttcg atccagcttt gggccagata aggcagtttc ggatcgttat
    64321 tgcgcaacgt cacgctgatt gatttgtcgc tttcattaaa tatcaaacgg gtacgatctg
    64381 gcgttacaga agcctgggcg acaaactagc agcgctaagc agtaacgcta gcgtcgtagc
    64441 acccaatttg gcagagcaaa gtaaatcaga catggatcat tccttaatca cgttattggt
    64501 tagctgaaac agaaacaggg ggattggtgg gtgtcacctg ttgcgtctgc tcctgaatga
    64561 caggctttat gtcatcagga acaaccggcg cgacgttgcc tttatgctgg catggcagta
    64621 acaagccgtt aaacagatcg gcaggcagcg ggtccggcag gttgatatcg caatgcgcaa
    64681 caccactcca gaacacactc atgtgttcgc caggtttcac cccagccaga taaacattgc
    64741 cgtcatcatc gacaaggccc actgtctgct cgttatcatt ttttacttct gcgccaaacg
    64801 gaggatggct gccgtcttgt aaacgcagca ccgccatcgc tttttgacca ctgatgacgg
    64861 caaatttgcg gtagccaatc gccccttcag ttagcgtggc ttgtaccacc gactgggttg
    64921 cttcggcgtt ttccggcagt ttgttgaggt cgatatacgc ctgattgcgg taatagttat
    64981 tgacgtcaga aacgacggct ttaccaaaca tattggtata aacagcagca ccgttacctt
    65041 ctaccggaac atcggcaacg ccatcggcat caatcaacaa gcgtgtcccg cccatattct
    65101 gggtacggtg aagtgcgcca ccgtgggcag taagcgttgc gccgccctgt aacgacaagc
    65161 ccgcagaggt gtattgccct tcatggtagt tcgcactgag gtcaacctgc gccagcgaac
    65221 catcatggct gtaatagccg tcaacgctgg tgtgtttgtc actggtgccg acgttcaact
    65281 gatagtgagt cgcgtcatcg acacggctga aataaccgac ctgactgctg tccgtcccac
    65341 tgccatagtt accgttatag ctaacggtac tgttgtcacc ccacggcata ctgagcgaaa
    65401 tgtacatgcc tttgtcggcc tggttgtcat actcgtaacg gtagccagtc attgagatgc
    65461 tgacattacg aatactgccc atattgaaat agtgcgagag catgatgttg tagtttgtct
    65521 gctcctcgcg atcccagtag gtatggcggg tatagttgag ataaaccgaa acacccgcat
    65581 cgcggaagtt ctggttataa gtggcagtgt acatctcttt gtcgttgccc gtgcgtacca
    65641 ttccgctgtc actggcatcc agatactcgc tcatggtcat aaagttctct tccgagaagc
    65701 gatatccagc aaaagtaacg cggctgttga gctggtcaaa gtctttggaa taactcacac
    65761 ggaaggagtt accgtccagc gaacctttgc cataagcggt gtctttatcc agtttggtgt
    65821 gcgagtgagt aacatcaaac gcgaccgcgc cgaatgtaga caaatcgcga ccgacgccaa
    65881 gcgccgcaga ctgatagttt tcatctccca gtgcgccgcc atataacgac cagccgttag
    65941 cgatccccca ggaagcttca gcaccagaaa aaaattcacc ctcgacatgg tgtccccact
    66001 cttgcggacg gcccatcatg atcttatagc gaacctgacc tggacgagtg aggtatggca
    66061 tcgaggcggt gctgatgtca tattcctgca cctggccgtt ctgttcttca atgcgaatat
    66121 gcaacgtacc ggagacagaa tcaccaagat cctgaatacg aaacggtcca gccggaacct
    66181 gcgtttcgta aatcacacgc cccatctggc tgacggtcac ttttgctgtg gtgtgtgcca
    66241 cgccggaaat gtctggcgcg tagccgcgca gattgggagg caacatttga tcgtcagtac
    66301 tgacactgcc accaacatag ttaaaaccat caaaaatatc ggatctgagg taatcctcgc
    66361 ccagcgccag tttggctttt aatgatggta acgcccgcta ggcatagtag cgactccact
    66421 cccatttttt ttgagtttca tcgccgctga attcatcgtc atcattactg cgagtatgtt
    66481 gatagttagt ctgccagtca gcacgcatac gccacggccc caggttaacc ccgaccgtcc
    66541 cgttgccgct gatctcgtta ctatcatcac cgccattttc ttcgtgccgg gtttgtgcgt
    66601 tgatgctgta gtccgcgacg atcccggaga tgccgtcatc ccaacgtgaa ggcggatccc
    66661 agtcgggata agtatattcg aggtaagcct gtggcagtga aatgactaat gcggactggc
    66721 ttaaatcagc cttaatttcc atgccttcca gttgaccgga tttcaggcat ttagcatcgt
    66781 ggctccattg cagatttttc gccacgtctt cttttaaacc aaactgcgct accagttccg
    66841 gtgtcagaca agcatagctt ttgctcgcgt catcttcacc agcataccag taaatatcgt
    66901 actcttccgc caatggctgt ttatttagtt gaacctgtaa attgtatttt ccgggctcaa
    66961 catatccctg gctggaaaaa cgcttcagat caatttttgt gtcgcctttt aattccagaa
    67021 aacgggaatc aaactgaatg tcgtcttcag cccagacact gctataactc cctgacattg
    67081 ccagcgcaat gcaccacgga agaatccgta atcgaaaaag agaatggtca ggcatacacg
    67141 tttctatccg tttttattct tgttcggtca aaatccattt aaatccccca tagcagtgtc
    67201 gctatggggg tatgccttac aggtaagtaa tctggaaggt ggtatttgct tcgaaattac
    67261 ccagatctgg cgcatcagct gcgccgacca gccaggcttt aaagttcagt gtctgcttcg
    67321 ctttgccttt attggtagaa gtgtctttca ctattttctg gtcaataccc atgccgcttt
    67381 tgtaagaggt acccagagag tcaccgatcg ccaggctaac attgttaaat gccgcacagg
    67441 tatcggtatt gaaaatggtg taataattgc cgctatttgc agaagaaacg gtaccagtaa
    67501 aggtagtggt cattgtttcc tgagtatcaa atacgcaatc ttgcagacga atctggaaac
    67561 ttttcggcgt ggtggtgccg ttatttttca gtttttcggc accgatatca cccatatcaa
    67621 cttccagttt gtgatcgtcc ggaacgatag aacacactga gtcttcaatg gtgccgtaaa
    67681 aacgtgcagt accgttgtta cccgcagcga ttgttgccgt agaggtgaac acgcccatca
    67741 ccattgccgc agcaatagct gttttaacaa acttactcat tatttgtcct taaactataa
    67801 ataattaaaa atataaacat tgcaatacat tgattcagtc aataaccaat gtttataccc
    67861 agccattgaa tacggcatgc gatccaaaaa gaacaggtcg tagaaaattg tattcgttgg
    67921 tgtggggccg catagtgatg tttccacacc cctgatgcaa tttaatttta ctaaagagta
    67981 aatgtagatt taatta
//
LOCUS       DAESHI010000007        65646 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.007, whole genome shotgun sequence.
ACCESSION   DAESHI010000007 DAESHI010000000
VERSION     DAESHI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65646
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.007"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..616
                     /locus_tag="KW011_000468"
     CDS             <1..616
                     /locus_tag="KW011_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH7658901.1"
                     /translation="IVIGIDNTYPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLF
                     DRQLDFIISPQHVSARVQELENLTISELPPLRLGFLVSRRYEERQEQELLQELPWLQM
                     RFQNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNP
                     ATLKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
     gene            complement(613..2091)
                     /gene="scpC"
                     /locus_tag="KW011_000469"
     CDS             complement(613..2091)
                     /gene="scpC"
                     /locus_tag="KW011_000469"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HBH7658902.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(2115..2900)
                     /gene="scpB"
                     /locus_tag="KW011_000470"
     CDS             complement(2115..2900)
                     /gene="scpB"
                     /locus_tag="KW011_000470"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HBH7658903.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(2911..3906)
                     /gene="meaB"
                     /locus_tag="KW011_000471"
     CDS             complement(2911..3906)
                     /gene="meaB"
                     /locus_tag="KW011_000471"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HBH7658904.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(3899..6043)
                     /gene="scpA"
                     /locus_tag="KW011_000472"
     CDS             complement(3899..6043)
                     /gene="scpA"
                     /locus_tag="KW011_000472"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HBH7658905.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(6246..7139)
                     /gene="argP"
                     /locus_tag="KW011_000473"
     CDS             complement(6246..7139)
                     /gene="argP"
                     /locus_tag="KW011_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HBH7658906.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            7569..8228
                     /gene="rpiA"
                     /locus_tag="KW011_000474"
     CDS             7569..8228
                     /gene="rpiA"
                     /locus_tag="KW011_000474"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBH7658907.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            8484..9716
                     /gene="serA"
                     /locus_tag="KW011_000475"
     CDS             8484..9716
                     /gene="serA"
                     /locus_tag="KW011_000475"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBH7658908.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            9945..10004
                     /gene="ibsC"
                     /locus_tag="KW011_000476"
     CDS             9945..10004
                     /gene="ibsC"
                     /locus_tag="KW011_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HBH7658909.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            10336..10395
                     /locus_tag="KW011_000477"
     CDS             10336..10395
                     /locus_tag="KW011_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBH7658910.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(10496..11098)
                     /locus_tag="KW011_000478"
     CDS             complement(10496..11098)
                     /locus_tag="KW011_000478"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBH7658911.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(11119..11302)
                     /gene="ssrS"
                     /locus_tag="KW011_000479"
     ncRNA           complement(11119..11302)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KW011_000479"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(11344..11673)
                     /gene="zapA"
                     /locus_tag="KW011_000480"
     CDS             complement(11344..11673)
                     /gene="zapA"
                     /locus_tag="KW011_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBH7658912.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            11841..12419
                     /gene="ygfB"
                     /locus_tag="KW011_000481"
     CDS             11841..12419
                     /gene="ygfB"
                     /locus_tag="KW011_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HBH7658913.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            12445..13770
                     /gene="pepP"
                     /locus_tag="KW011_000482"
     CDS             12445..13770
                     /gene="pepP"
                     /locus_tag="KW011_000482"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HBH7658914.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNESEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            13767..14945
                     /gene="ubiH"
                     /locus_tag="KW011_000483"
                     /gene_synonym="visB"
     CDS             13767..14945
                     /gene="ubiH"
                     /locus_tag="KW011_000483"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HBH7658915.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            14968..16170
                     /gene="ubiI"
                     /locus_tag="KW011_000484"
                     /gene_synonym="visC"
     CDS             14968..16170
                     /gene="ubiI"
                     /locus_tag="KW011_000484"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HBH7658916.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(16244..16327)
                     /locus_tag="KW011_000485"
     CDS             complement(16244..16327)
                     /locus_tag="KW011_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7658917.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            16617..17711
                     /gene="gcvT"
                     /locus_tag="KW011_000486"
     CDS             16617..17711
                     /gene="gcvT"
                     /locus_tag="KW011_000486"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HBH7658918.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            17735..18124
                     /gene="gcvH"
                     /locus_tag="KW011_000487"
     CDS             17735..18124
                     /gene="gcvH"
                     /locus_tag="KW011_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBH7658919.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            18243..21116
                     /gene="gcvP"
                     /locus_tag="KW011_000488"
     CDS             18243..21116
                     /gene="gcvP"
                     /locus_tag="KW011_000488"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HBH7658920.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDCFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            21382..22125
                     /locus_tag="KW011_000489"
     CDS             21382..22125
                     /locus_tag="KW011_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBH7658921.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(22182..23615)
                     /gene="bglA"
                     /locus_tag="KW011_000490"
     CDS             complement(22182..23615)
                     /gene="bglA"
                     /locus_tag="KW011_000490"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HBH7658922.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLNVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            23660..23971
                     /locus_tag="KW011_000491"
     CDS             23660..23971
                     /locus_tag="KW011_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBH7658923.1"
                     /translation="MQPNDITFFQRFLDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            24135..24794
                     /locus_tag="KW011_000492"
     CDS             24135..24794
                     /locus_tag="KW011_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBH7658924.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(24990..25970)
                     /gene="ygfZ"
                     /locus_tag="KW011_000493"
     CDS             complement(24990..25970)
                     /gene="ygfZ"
                     /locus_tag="KW011_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HBH7658925.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            26213..26479
                     /gene="sdhE"
                     /locus_tag="KW011_000494"
     CDS             26213..26479
                     /gene="sdhE"
                     /locus_tag="KW011_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HBH7658926.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            26460..26867
                     /locus_tag="KW011_000495"
     CDS             26460..26867
                     /locus_tag="KW011_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HBH7658927.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(26907..27428)
                     /gene="fldB"
                     /locus_tag="KW011_000496"
     CDS             complement(26907..27428)
                     /gene="fldB"
                     /locus_tag="KW011_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HBH7658928.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            27540..28436
                     /gene="xerD"
                     /locus_tag="KW011_000497"
     CDS             27540..28436
                     /gene="xerD"
                     /locus_tag="KW011_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBH7658929.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            28461..29171
                     /gene="dsbC"
                     /locus_tag="KW011_000498"
     CDS             28461..29171
                     /gene="dsbC"
                     /locus_tag="KW011_000498"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HBH7658930.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            29177..30910
                     /gene="recJ"
                     /locus_tag="KW011_000499"
     CDS             29177..30910
                     /gene="recJ"
                     /locus_tag="KW011_000499"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBH7658931.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            31001..32099
                     /gene="prfB"
                     /locus_tag="KW011_000500"
     CDS             join(31001..31075,31077..32099)
                     /gene="prfB"
                     /locus_tag="KW011_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBH7658932.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            32109..33626
                     /gene="lysS"
                     /locus_tag="KW011_000501"
     CDS             32109..33626
                     /gene="lysS"
                     /locus_tag="KW011_000501"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBH7658933.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(33669..34217)
                     /gene="idi"
                     /locus_tag="KW011_000502"
     CDS             complement(33669..34217)
                     /gene="idi"
                     /locus_tag="KW011_000502"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBH7658934.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            34272..34343
                     /locus_tag="KW011_000503"
     CDS             34272..34343
                     /locus_tag="KW011_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7658935.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(34340..34465)
                     /locus_tag="KW011_000504"
     CDS             complement(34340..34465)
                     /locus_tag="KW011_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7658936.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(34467..35545)
                     /locus_tag="KW011_000505"
                     /pseudo
     CDS             complement(34467..35545)
                     /locus_tag="KW011_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            35981..37900
                     /gene="ygfT"
                     /locus_tag="KW011_000506"
     CDS             35981..37900
                     /gene="ygfT"
                     /locus_tag="KW011_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HBH7658937.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            37900..38388
                     /locus_tag="KW011_000507"
     CDS             37900..38388
                     /locus_tag="KW011_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH7658938.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(38457..38807)
                     /gene="ghxQ"
                     /locus_tag="KW011_000508"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             complement(38457..38807)
                     /gene="ghxQ"
                     /locus_tag="KW011_000508"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(38843..39157)
                     /locus_tag="KW011_000509"
     CDS             complement(38843..39157)
                     /locus_tag="KW011_000509"
                     /inference="COORDINATES: protein motif:HMM:NF014081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBH7658939.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            complement(39090..42029)
                     /locus_tag="KW011_000510"
     CDS             complement(39090..42029)
                     /locus_tag="KW011_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HBH7658940.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            complement(42026..42805)
                     /locus_tag="KW011_000511"
     CDS             complement(42026..42805)
                     /locus_tag="KW011_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HBH7658941.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(42856..44184)
                     /gene="ssnA"
                     /locus_tag="KW011_000512"
     CDS             complement(42856..44184)
                     /gene="ssnA"
                     /locus_tag="KW011_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBH7658942.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(44187..47285)
                     /gene="ygfK"
                     /locus_tag="KW011_000513"
     CDS             complement(44187..47285)
                     /gene="ygfK"
                     /locus_tag="KW011_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HBH7658943.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCNKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYEDKITVFSLAQDFDNSS
                     TPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(47607..48185)
                     /gene="mocA"
                     /locus_tag="KW011_000514"
     CDS             complement(47607..48185)
                     /gene="mocA"
                     /locus_tag="KW011_000514"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBH7658944.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            48288..49058
                     /gene="yqeC"
                     /locus_tag="KW011_000515"
     CDS             48288..49058
                     /gene="yqeC"
                     /locus_tag="KW011_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBH7658945.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            49106..50731
                     /locus_tag="KW011_000516"
     CDS             49106..50731
                     /locus_tag="KW011_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBH7658946.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVEWKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILRVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(50772..51704)
                     /gene="arcC"
                     /locus_tag="KW011_000517"
     CDS             complement(50772..51704)
                     /gene="arcC"
                     /locus_tag="KW011_000517"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBH7658947.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(51752..53137)
                     /gene="hyuA"
                     /locus_tag="KW011_000518"
     CDS             complement(51752..53137)
                     /gene="hyuA"
                     /locus_tag="KW011_000518"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HBH7658948.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(53190..54401)
                     /locus_tag="KW011_000519"
     CDS             complement(53190..54401)
                     /locus_tag="KW011_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBH7658949.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(54459..55655)
                     /gene="dpaL"
                     /locus_tag="KW011_000520"
     CDS             complement(54459..55655)
                     /gene="dpaL"
                     /locus_tag="KW011_000520"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBH7658950.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFPQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(55713..56900)
                     /gene="ygeW"
                     /locus_tag="KW011_000521"
     CDS             complement(55713..56900)
                     /gene="ygeW"
                     /locus_tag="KW011_000521"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HBH7658951.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            57379..59157
                     /locus_tag="KW011_000522"
     CDS             57379..59157
                     /locus_tag="KW011_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HBH7658952.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTSASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(59197..59676)
                     /gene="xdhC"
                     /locus_tag="KW011_000523"
     CDS             complement(59197..59676)
                     /gene="xdhC"
                     /locus_tag="KW011_000523"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HBH7658953.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(59673..60551)
                     /gene="xdhB"
                     /locus_tag="KW011_000524"
     CDS             complement(59673..60551)
                     /gene="xdhB"
                     /locus_tag="KW011_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HBH7658954.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(60562..62859)
                     /gene="xdhA"
                     /locus_tag="KW011_000525"
     CDS             complement(60562..62859)
                     /gene="xdhA"
                     /locus_tag="KW011_000525"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HBH7658955.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            63274..64029
                     /locus_tag="KW011_000526"
     CDS             63274..64029
                     /locus_tag="KW011_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBH7658956.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            64108..64177
                     /locus_tag="KW011_000527"
     tRNA            64108..64177
                     /locus_tag="KW011_000527"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:64140..64142,aa:Gly,seq:ccc)
     gene            64370..>65646
                     /locus_tag="KW011_000528"
     CDS             64370..>65646
                     /locus_tag="KW011_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7658957.1"
                     /translation="MEYFMKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYM
                     STLDQIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQND
                     ALGLYLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDAR
                     LNTSSVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYE
                     RITLQLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAG
                     PYGIKRYEKDNYQSANFWFNDIKTDTDQNSHAK"
BASE COUNT        15770 a        16324 c        17397 g        16155 t
ORIGIN      
        1 aattgtgatt ggcatagata atacttatcc gacaattatt ttcgatcagt tgattagtct
       61 cggagataag tatgaaggcg tgaccgccca gccagttgag ttcagcgaaa acggcgttat
      121 cgataatctt tttgaccgtc agttagattt tattatctcc ccacagcatg tatctgcacg
      181 agttcaggaa ctggaaaacc tgactatcag tgaattacca ccgctgcgat tgggctttct
      241 cgtttctcgc cgttatgaag agcgtcaaga gcaagagctg cttcaggagc tgccctggct
      301 acaaatgcgc tttcaaaacc gggctaactt cgaagcaatg attgatgcga atatgcgacc
      361 ttgtgggatc aatcccacca ttatttatcg tccctacagt tttatggcca aaatcagcgc
      421 cgtggagcgc ggacattttc tgacggtgat cccgcatttt gcctggcgac tggtgaatcc
      481 ggcaacgctg aaatattttg atgcgccgca caggccgatg tatatgcagg aatatcttta
      541 ttcaatcaga aatcatcgtt ataccgccac gatgcttcag catattgctg aagatcgtga
      601 cgggacaaac cattaaccca gcatcgagcc ggttgcaatt aaattacggt gtaagtcgaa
      661 gacgtggcta agatcgtggt gaatatgtcc gccgggcgca ttttccagat agcgatgcag
      721 ataatcccga tacataggat gtgcacaatt atcaatgata gtgcgggcgc gttgaagcgg
      781 ggaaagaccg cgtagatcgg cgatcccttg ttcggtgatg atcactttga cgctgtgttc
      841 gctgtgatca acatggctgc acattggcac gacggttgag atcttgcctt ctttagcaat
      901 cgacggagcc ataaagatcg acagatacgc gttgcgttca aaatcaccgc tgccgccgat
      961 gccgttcatc agatcgaccc cggctacgtg cgttgaattt gcatgcccgt aaatatcaaa
     1021 ctccaggccg acgttcagag cgatgacgcc cagacgacgg atgatttccg ggttattgga
     1081 aatctcctgc ggacgcaaca caatgcggct ggcaaagtaa tccatattgt cgtaaatctt
     1141 gcgcagggaa tcggccgaga ttgtcaggct ggaggcgctg gccccgctga ttttgccggt
     1201 ttccagtaaa tgcaccaccg attcctgtag cacttccgaa tacatcatga acggaggaat
     1261 ttccgggttt tcccccagac gcgccattac cgcattattg atattgccca cgccactttg
     1321 cagcggcaga aattccggcg gaatacgccc atgcgccatt tcctgcaata agaacgtgac
     1381 cacgttatcg gcaatctgct ggcacatggg attttgctta tccagcatat taccggcgtc
     1441 gggcaagttg gtttccacga cggcgacaat ctttttcgga tcgatttgca catagcgggt
     1501 accgacgcga tccattgcat ggaagatcga cacgctattg cgccgcggtg gtgcgccagg
     1561 aatcacaata tccgccagtt ctgcaacgcg cggatcgtga tagtgattaa gttcaatgat
     1621 cactttcttc gcccgcagca gccatgtcgg cgcattaccg atcccgctgg ttaaccagac
     1681 tcgaccatcc ggtgccagtg ccgatgcttc gatgacggca acatcaatgt cgccgaagaa
     1741 accgtaattg accatttgcg ccacttcgct caaatgcagg tcaacgaaac tcaccgcgcc
     1801 ctgattgatc tttttacgta aaccggacga tgtttgatat ggcgcacgcc aggaaacagc
     1861 atcggcgtca gaaagtacat cgtcagcggc ggcgctgatt gacgcccccg tcagcaggcg
     1921 aatttgatac ggctttttgg cctcatgctg ttcgttagct ctgcgggcaa tcgcggtcgg
     1981 taaggctttc ggcgaacccg ctggggtaaa gccgctaaat gccaccatgt cgttatgctg
     2041 gataatttct gccgcttcat cggcggtcat ccttgtccac tgagtttcca tttttactcc
     2101 ttcgttcgca gggattaatg accaacgaaa ttaggtttac gtttttcgag gaaagcgttc
     2161 atcccttcct ggtaatcttc gctgtcataa accgcgcggc gcatcccctg aatacgttca
     2221 aattcatcgg agttcatggt gtgcgcttcg cccagtacac gcagctcttc tttgataaca
     2281 gcaatggcta acggcgcttt ctcagagatg tggtgcgcca tttgtaaggt gaaatcttcc
     2341 agttcttcca cttccacaac atggttgagg atgccgacag ccaacgcgcg ctgggcggtg
     2401 attggcgaag cggtaaaaat cagctctttg acaatgtgga agcccgcgtc gcgggtcagg
     2461 ttgtgaatgc cgaccaggtt atacgggacg ccgaggttta caggcatcat tgagaaggtt
     2521 gaggtactgg cggcgatgat cagatcggaa ctcatgatca tttcaaatgc gccaccccaa
     2581 acactacctt ccaccatcga aatgatcggt ttcgggaatt tttggatcat gcgggtgatt
     2641 tgacgcaatg gatcatcata ggagagcgga tcgcgaccgc cagacggcag ttcgtgaata
     2701 tcgtgacctg cggagaagac tttggatcca cttggcgccc gcaaaatgat acagcgaatt
     2761 tccggccggt tgagatcgct taacgcctgc ataagatcat caataaagac tttacttaag
     2821 gcattaagtt ttcgtccata gttaaactca atgaccgcca ctttgttgat agtggcaacg
     2881 ttcacatact gataagacat aaaaattcct ttaatcaaaa tattgcgtct ggataaattc
     2941 actgagctgc cgcaggccgg tgcgcggtga gagcgtattg tttttgaccg ctaaaagcgt
     3001 ctggcggtaa tagcgatcga aatcttcatt cgcgaacagg tgattcagta cttcttcttc
     3061 ggtctgctta cgcagccatt ccaccgattg ttgttgccgc acttgttgta aacgaccact
     3121 ggcagttagc gcggttttga agtcgatgat ggcgtgccag atctcatcga ttccacgttt
     3181 ttccagtgcg ctacaagtca gaacccgtgg ctgccattcg tcgtatttac gtcgcagaat
     3241 atgcagggca ctctcgtaca tatgccgggc aatggcgaca ttggtatggt tatcgccatc
     3301 gtctttgttg ataacgagca gatcagccac ttccatcagc ccttttttaa tgccctgcag
     3361 atcatcgccg ccaccggcaa tttgcaacga gataaaacag tccaccatgc gggcgacttc
     3421 cgtttccgac tgcccgacgc caaccgtttc gacaatcact acgtcataac ccgccgcttc
     3481 gcataacagc attaattccc gtgctcgctg actggcaccg cccagatgac cggaggaggg
     3541 taccgggcga ataaacgccg cctcggcacg cgccaggtca ttcatgcggg ttttatcccc
     3601 gagaatgcta ccgccagtga ccgggctgct gggatcgacc gcaataacag caacctttaa
     3661 tccctcacga atcaacaaca tgccaaaggc ttcaagaaag gtacttttcc ccgcgccggg
     3721 tgtgccggta acgcccagtc gcagggcgtt accacagaac ggcataatgg cgtcaagcag
     3781 ctgtgtactt agcgcctgat gacgtgggtg acggctttcc accagcgtca tggcctgggc
     3841 gagcgtggca cgctcaccct gacgtaagcg gcgaatactt tctgccagcg tggcttcatt
     3901 aatcatgatg ctggcttatc agattcagta cgtcgcgcac actgtcgagc ataggtgtac
     3961 ctggaccata aatcgccgcc acgccgcgct cttgcaggaa ggcgtaatcc tgcggcggaa
     4021 tgacgccacc cgcgaccacg cagatatctt cgcgtcccca ttttttcagc gcttcgacca
     4081 gttccgggat cagcgtttta tgatcggcag ccagtgagga tgcgcccact acgtgaacgt
     4141 cgttttctac ggccaggcgg gcgatctctt caggtgtaga gaacatcggg cttaaatcta
     4201 cgtcgaaacc gagatcggaa taggcgctgg cgatcacttt cgcgccgcga tcgtgtccat
     4261 cctggcccat cttagcgatc agaatgcgcg ggcgacgacc attgtcggca aggaactgct
     4321 ccgtttgcgc aacaatggca tcgaactcgg aggccgattt ctcagactga tgatagcttt
     4381 gcgcaatcac gccggtaaca cactggcttg gcaccagata acggtcgaaa gcgacttcca
     4441 gcgcatcgga aatttcaccc agggtggcgc gaacgcgagc ggcattaaca gcggcagcca
     4501 gcaggttttc gttatgctgt gcggcgtgag tcagggcgtt caacgcggcg gttacggcgg
     4561 catcatcacg ggtggcgcga atgcgttcca gcgaagcaat ttgctcgtta cgcaccatca
     4621 cgttgtcgat ctcaagtaca tcggtttcgt cttcgtgatc cagtttgtac ttgttgacac
     4681 caacgatgac acgcttgcct tggtcgatca gcgactgttc gcgcgctgag gcctcttcga
     4741 tcattcgttt tggcagacct gcttcgatcg ctttcgccat gccaccggct tcgtcgatct
     4801 gttggataat agctctggct tgtttgacga tttgatcggt cagcgactca atgtaatagg
     4861 atccggccag tggatcgacg gtgcggcaga gttctgattc ttcctggatg atgatctggg
     4921 tgttgcgggc aatgcgtgct gagaaatcgg taggcaaacc aagcgcttcg tcaaaggcgt
     4981 tggtatgcag tgactgagta ccgcccagcg tcgcagccag tgcttcaatg gtggtgcgga
     5041 taacgttgtt atacggatcc tgttcagtca ggctccagcc tgaggtctgg cagtgggtac
     5101 gcagcgccag tgatttcgga tcctgtgcgc caaatccact gaccgcttcg ctccataaat
     5161 aacgtgccgc acgcaacatg gcgacgttca taaacagatc catgccgata ccgaagaaga
     5221 acgacaggcg aggagcgaag tcatcaattt tcagtccggc agagattgct gctttgatgt
     5281 actcaatccc atcggcgagc gtaaatgcta cctgctgcac gcagttggca cccgcttccc
     5341 ccatgtggta accgctgata ctgatggtat taaatcgcgg catgttgccg gaacaccagg
     5401 cgatgatgtc ggcgataatg cgcattgacg gttttggtgg gtaaatatag gtgttgcggc
     5461 agaggtactc tttgagaata tcgttttgaa tggtgccggt cagtttatca ggtgtaacac
     5521 cttgctcttc tgcggcgacg atataaaacg ccagtactgg tagcactgcg ccattcatgg
     5581 tcatcgaaac cgacatttta tccagcggga tctggtcgaa caggactttc atatcttcca
     5641 cggtgtcgat agcgacgccc gctttgccga cgtcacccgc cacgcgtggg ttatcggagt
     5701 cgtagccacg gtgggtggca aggtcaaacg caacggaaag acctttttgc ccggcggcca
     5761 ggttacggcg ataaaaagcg ttggactctt ttgctgttga aaaaccagca tactgacgga
     5821 tggtccacgg ttgggcggta tacatagtgg cacgcgggcc acgaacgtag ggcggcaaac
     5881 caggaagggt acctgtcacc tccagattat cgagatcggc ttcggtatac agctgcttga
     5941 tggcgatccc ttccgcggtt tgctgaacca gcgagtcgac agttttctcc cgacggctca
     6001 attccttgtt ggcaagctgt tgccactcct gctcgttaga cattatttgc tccgtaaaac
     6061 gcaatcaaaa tgagggtatt tgcgcttacg gtgagctttt tacgtgggtt tgtcgccagc
     6121 aaaaaaatgc attcagacgt cgttttttgc cgcctgagat ttgtctattt tgtgatgtga
     6181 ttcaaggaat acaaaaagaa gtgaggataa cgcctgatat gtgctatcag gcattatttg
     6241 atggattaat cctgacgaag gactttgtga ccataatcga gcaacgcatc agtgacttta
     6301 cgcatcatgc ggctttcagg agcaaagcgg tgccagtaga gcatccggcg ttgaaatagc
     6361 cctggcgtta agtcaatcag ttcaccgctg gctagttctt tctcgatttg caggtgcggg
     6421 atcatacagc aggtggtgcc ctggcgagca agttgcacga acgcttctga agaattaacg
     6481 atatggcagg gcacgctgcc tggaggcaga tcgaagtttt gctgcaaaaa ggcctggtgc
     6541 atatcgtcaa gatggtcaaa ggcgaccaca ggtgctttca gtaatgccga acgcgttacg
     6601 ccgttaggga aatatttttc ggcaaagggt tttgagctga cgaacagata gtcgagcgca
     6661 ccaagtttat cgacaagaca actcggcagc gcctgatgtt gaatactcac cgcgccgacc
     6721 acttcgccgc ggcgcagacg ttcctgagta cgggtttcat cttctacctg caagttgagg
     6781 cggataggcg aatcagccaa cacaggagcc agtgccggaa gcaaccacgt cgccagactg
     6841 tcggcgttga ccgccagtga aagcagcagc ggagtcgaac cggtttgttc atcgcccagc
     6901 cactcttctt ccagcaactc cacctggcgc agcagtgcca gcagtttttg cccttgttcc
     6961 gtcgggcgcg gcggtacggt acgcaccaac agcggctgcc cgaacatatt ttccagttgc
     7021 ttaatgcgct gtgagacggc tgattgtgta atgcacagct tttgtgccgc gcgctcaaat
     7081 cctcgttcac gtatcaccgc atccagtgcc tgtaatgttc tgtagtccgg gcgtttcatt
     7141 gctgttgtct gctcctggta aatgcttttt cctgcactat gacacaattt tgtgtcaggt
     7201 tgcatatacc gaagccgtcg gcgggtaaca tttgtatccg tggttaaacg cgcagcttaa
     7261 aagatcaggg atttgcgcta atgcatttcg ctcagcgagt tcgtgccctg gttgtgttga
     7321 atggggtggc gttattgcct caatttgcct gtaaacaggg gcttgcgaac ggggggaact
     7381 ggtgcgcctg ttcgcaccgt ggagcggcat acccagaccg ttgtatgctt tatttgcggg
     7441 gcgaaagggg atgcccgcca ttgcgcgata ttttatggat gagttaacca cgcggcttgc
     7501 caacggggtc tgaatcgctt tttttgtata taatgcgtgt gaaatttcat accacaggcg
     7561 aaacgatcat gacgcaggat gaattgaaaa aagcagtagg atgggcggca cttcagtatg
     7621 ttcagcccgg caccattgtt ggtgtaggta caggttccac cgccgcacac tttattgacg
     7681 cgctcggtac aatgaaaggc cagattgaag gggccgtttc cagttcagat gcttccactg
     7741 aaaaactgaa aagcctcggc attcacgttt ttgatctcaa cgaagtcgac agccttggca
     7801 tctacgttga tggcgcagat gaaatcaacg gccacatgca aatgatcaaa ggcggcggcg
     7861 cggcgctgac ccgtgaaaaa atcattgctt cggttgcgga aaaatttatc tgtattgctg
     7921 acgcttccaa gcaggttgat attctgggca aattcccgct gccagtagaa gttatcccga
     7981 tggcacgtag tgcagtggcg cgccagctgg tgaaactggg cggtcgtccg gaataccgtc
     8041 agggcgtggt gaccgataac ggcaacgtga tcctcgacgt ccacggcatg gaaatccttg
     8101 acccgatagc gatggaaaac gccataaatg cgattcctgg cgtggtgact gttggcttgt
     8161 ttgctaaccg tggcgcggac gttgcgctga ttggcacacc tgacggtgtc aaaaccattg
     8221 tgaaatgatc tgacggggga acctcccccg ttaaaaaaat tctcttcatt aaatttggtg
     8281 acatgtgtca cgtttttact tggctattgt cgattgctct aaataaatcc tctaaaccag
     8341 catattcaac caagaaatac ctttgcgtga tatttcctca acatcgcgac gcaaacgttc
     8401 atattgccgc aatattattt tttgatatgt tgaaaggcgg atgcaaatcc gcacacaaca
     8461 tttcaaaaga caggattggg taaatggcaa aggtatcgct ggagaaagac aagattaagt
     8521 ttctgctggt agaaggcgtg caccaaaagg cgctggaaag ccttcgtgca gctggttaca
     8581 ccaacatcga atttcacaaa ggcgcgctgg atgatgaaca attaaaagaa tccatccgcg
     8641 atgcccactt catcggcctg cgatcccgta cccatctgac tgaagacgtg atcaacgccg
     8701 cagaaaaact ggtcgctatt ggctgtttct gtatcggaac aaaccaggtt gatctggatg
     8761 cggcggcaaa gcgcgggatc ccggtattta acgcaccgtt ctcaaatacg cgctctgttg
     8821 cggagctggt gattggcgaa ctgctgctgc tattgcgcgg cgtgccggaa gccaatgcta
     8881 aagcgcaccg tggcgtgtgg aacaaactgg cggcgggttc ttttgaagcg cgcggcaaaa
     8941 agctgggtat catcggctac ggtcatattg gtacgcaatt gggcattctg gctgaatcgc
     9001 tgggaatgta tgtttacttt tatgatattg aaaacaaact gccgctgggc aacgccactc
     9061 aggtacagca tctttctgac ctgctgaata tgagcgatgt ggtgagtctg catgtaccag
     9121 agaatccgtc caccaaaaat atgatgggcg cgaaagagat ttcgctaatg aagcccggct
     9181 cgctgctgat taatgcctcg cgcggtactg tggtggatat tccggcgctg tgtgatgcgc
     9241 tggcgagcaa acatctggcg ggggcggcaa tcgacgtatt cccgacggaa ccggcgacca
     9301 atagcgatcc atttacctct ccactgtgtg aattcgacaa cgtccttctg acgccacaca
     9361 ttggcggttc gactcaggaa gcgcaggaga atatcggcct ggaagttgcg ggtaaattga
     9421 tcaagtattc tgacaatggc tcaacgctct ctgcggtgaa cttcccggaa gtctcgctgc
     9481 cactgcatgg tggtcgtcgt ctgatgcaca tccacgaaaa ccgtccgggc gtgctaactg
     9541 cgctgaacaa aatcttcgcc gagcagggcg tcaacatcgc cgcgcaatat ctacaaactt
     9601 ccgcccagat gggttatgta gttattgata ttgaagctga cgaagacgtt gccgaaaaag
     9661 cgctgcaggc aatgaaagct attccgggta ccattcgcgc ccgtctgctg tactaattcc
     9721 ccttctctga aaattaacgg gcaggtcact gacttgcccg tttttttatc ccttctccac
     9781 accgttgccg cgttgcgcat tctactttgc gagtctgctc cagaaaataa atcaaactca
     9841 gatcaatgcg ttgtgtttat ttaatgggga tttacgatgg cgggacagca tggggctgta
     9901 acggtaaagc cctcaccgaa gcgagggctt aaaggagaag ggttatgatg cgacttgtca
     9961 tcatactgat tgtactgtta ctgataagtt tcggagctta ttaacagtca gtctcagggg
    10021 aggagtcatc ctctcttacc cttactcact aaattaggtc aaagaatcaa cgatgtcaat
    10081 cagggcgatg cggggtgcat cgccctgaac cgaacttttt gatagccact tcatcgccca
    10141 cgtttactgc cgtaatagat cctttttccc aaaaattatc tctgttttgc atgggttttt
    10201 cgagcacgct cgaagaaaag atttaaaggt agatcaatgc gttgtggtta tttaaagaga
    10261 gcttacgata gctgggcagc atggggctgt aacggtaaag ccctcaccga agcgagggct
    10321 tgaaggagaa gggttatgat gcgacttgcc atcatactga ttgtactgtt actcataagt
    10381 ttcccagctt attaacagtc agtcacaggg gaggagcaat cctcccttac ccttactcac
    10441 taaattaggt taaagaatca acgatgtcaa tcagggcgat gcaggtgtat cgcccttacc
    10501 actcccagac tttcgacggt gtaaccaccg caggaagagg gatatcccac tcttcaacgg
    10561 ggagtttttc caccaactga caatcatgcg cataacccac cggttgcgtt ttatagtgct
    10621 gccagttttg taaggtccga tcataaaaac caccgcccat tcccaggcgc tgaccgtact
    10681 catcaaaggc gaccagcggt gtgatcagca cgtctaatcg ggaaaggggt agcacgtcac
    10741 gcacatccag ttttggctca tggatcttca acctgttcat caccagttcg ctttgcggat
    10801 ggtaattcag gaacagcaaa ttaccggcac taaagggatg caaaactgga agatatacgc
    10861 gcttaccggc gcgccagagt tgttctatta gcggctgggt gtcgagttca ccatcaaaag
    10921 agaggaatac agcgaccgta tgcgccatca ccaccggggg ataagtcatc atccgggtag
    10981 cggcttgttg acccatttcc tgctgttgtt ccggcgttaa cgcacgacga cgttgccgaa
    11041 tcattttgcg gatttcttgt cgggataatg tcagtgggag ttctggtagt tgcgtcatgg
    11101 ctggtgccag ataagaaggg aatctccgag atgccgccgc aggctgtaac ccttgaaccc
    11161 ttggttcaag gtgaatgtgt cgtcgcagtt ttaaggcttc tcggacggac cgagcatgct
    11221 caccaaccgc ggagcgccac attcttgtgg tatgaaatat cggctcaggg gactggcccg
    11281 cttgcgaaca tctcagagaa attttgtctt cacggttact ctaccacagt aaaccgaaaa
    11341 gtgtcattca aagttttggt tagttttttc ggtgatgcga ccttgttcaa gtaacgcttg
    11401 ttctatggtc tgctgcagca tccgaatacg ctgttccata cttgccgcgt agtcacgagt
    11461 cttcgctttt tcttgcgcta actcatagct gatattcaat gcggcaatga agaccaactg
    11521 ttcagtattt gtgactctag tgcgttcttt cagatcttgc aaccgttggt tcagatcgtc
    11581 cgctgcctga ttcaacgcat ccctttggtc aggcgggcag ttcacacgca gtgaacggcc
    11641 aaaaatttgg atatcgacgg gttgtgcaga catgccacct tcctgctgat tgactgcgct
    11701 gccttcgtct ttagacccat ggtctgcgaa ggggcgacac tatagctacc ctgatgagaa
    11761 gagacaagcc cttttctggt ccaccagggc ccaaagtggt agcatatcat gaatattcct
    11821 ccctttgacg acgaatgctt atgtctatac agaacgaaat gcctggttac aacgaaatga
    11881 accagtatct gaaccaacaa gggacggggc tgacccctgc cgagatgcat ggtttaatca
    11941 gtgggatgat atgtggcggt aacgatgaca gctcatggct gccgctactt cacgacctga
    12001 cgaacgaagg catggctttc ggtcatgagc tggcacaggc actgcgtaaa atgcactctg
    12061 ccaccagcga tgccctgcag gatgacggct tcctttttca gctttatctg cctgatggcg
    12121 atgatgtcag cgttttcgat cgggctgatg cattggcagg ttgggtcaat cacttcctgc
    12181 ttggtcttgg cgttacgcaa ccgaagctgg ataaagtgac cggcgaaacc ggtgaagcta
    12241 tcgacgatct gcgtaacatt gcgcaactgg gttacgacga agacgaagat caggaagagc
    12301 ttgaaatgtc gcttgaagag atcatcgaat acgttcgtgt tgccgcgctg ttatgccacg
    12361 acacctttac tcatccgcaa ccgaccgcgc cagaagtaca aaaaccgact ctacactaaa
    12421 aacaaaaaac gtaaggagag tgttatgagt gagatatccc ggcaagagtt tcagcgtcgc
    12481 cgtcaggccc tggtggagca aatgcaaccc ggcagcgccg cgctgatttt tgctgcacca
    12541 gaagtaacac gtagcgccga cagcgaatac ccctatcgtc agaacagtga cttctggtac
    12601 ttcaccggct ttaacgaatc ggaagcggtg ctggtgctga ttaaaagcga tgacactcat
    12661 aaccacagcg ttctgtttaa ccgcgttcgc gacctgacgg cggagatctg gtttggccgt
    12721 cgcttaggcc aggatgccgc gccagagaaa ctgggcgttg accgcgcact ggcattcagc
    12781 gaaatcaatc agcaacttta tcaactactt aacggcctgg atgtggttta ccatgcccag
    12841 ggcgaatatg catatgctga tgaaatcatg aacagtgcgc tggaaaaact gcgtaaaggt
    12901 tcgcggcaaa atctcaccgc accggcaacg atgatcgact ggcgtcctgt tgttcatgaa
    12961 atgcgcctgt tcaaatcgcc agaagagatt gccgtactcc gccgcgcggg agaaatcacc
    13021 gccatggcac atacacgggc gatggaaaaa tgccgtccgg gaatgttcga gtaccatctg
    13081 gaaggcgaaa ttcaccacga atttaaccgc cacggtgcgc gctatccgtc ctataacacc
    13141 attgtcggca gcggtgaaaa cggctgcatt ctgcactaca ccgaaaacga gagtgaactg
    13201 cgcgacggtg acctggtgtt gattgacgcg ggttgtgaat acaaaggtta cgcgggcgat
    13261 attacccgca ccttcccggt caacggcaaa ttcacccagg cccagcgtga aatctacgac
    13321 attgtgctgg agtctctcga aaccagcctg cgcctgtatc gtccgggaac ttccattctg
    13381 gaagtcactg gtgaagtggt gcgcatcatg gttagcggcc tggtaaaact cggcatcctg
    13441 aaaggtgatg ttgatgaact gatcgctcag aacgcccatc gtcctttctt tatgcatggc
    13501 cttagccact ggttaggact ggatgtccat gacgttggcg tttatggtca ggatcgctcg
    13561 cgcattctgg aaccggggat ggtactgacc gtagagcctg gtctgtatat cgcgccggat
    13621 gcggatgtgc cagaacaata tcgcggtatc ggcattcgta ttgaagacga cattgtgatt
    13681 accgaaaccg gcaacgaaaa cctcaccgcc agcgtggtga aaaagccgga agaaatcgaa
    13741 gcgttgatgg cagctgcgag aaagcaatga gcgtaatcat cgtcggtggc ggcatggcgg
    13801 gcgcgacgct ggcgctggct atttcccggt taagtcacgg ggcgctgccg gtacatttga
    13861 ttgaagcgac cgcgccagag tcacatgctc atccgggctt tgatggacga gctattgcgc
    13921 tggctgcggg gacctgtcag caactggcgc gcatcggcgt ctggcaatct ctggcggatt
    13981 acgcaaccgc catcactacc gtgcatgtca gcgatcgtgg tcacgctgga tttgtcaccc
    14041 tcgccgcaga agattaccaa ctggcggcgc tgggacaggt tgtcgaattg cacaatgtcg
    14101 ggcaacggct gtttgcattg ctgcgtaaag cacctggcgt aacgctgcat tgccctgatc
    14161 gcgtggctaa cgttgcccgt actcagagtc acgttgaagt gacgctggag agtggcgaga
    14221 cgctgacggg ccgcgtgctg gtagcagctg atggcaccca ttcagcgtta gccaccgcgt
    14281 gcggcgttga ctggcagcag gagccttacg aacaactggc cgtgattgcc aacgttgcga
    14341 cttccgttgc gcacgaaggc cgcgcttttg aacgcttcac gcaacatggc ccgctggcga
    14401 tgttgccgat gtctgacgga cgctgttcgc tggtctggtg tcatccactg gaacggcgcg
    14461 aagaggtgct gtcgtggagc gacgagaagt tttgccgtga actccagtcg gcctttggct
    14521 ggcgacttgg aaaaattacc cacgccggta aacgcagtgc ttatccgctg gcgttaaccc
    14581 gcgccgccag agctattacc catcgtaccg tgctggtggg caatgcggcg caaactctgc
    14641 acccgattgc cgggcaaggg tttaacctcg gtatgcgaga tgtcatgagc cttgcggaaa
    14701 ccctgactca ggcgcatgag cgcggagaag acatcggtga ttacggcata ttgtgccgct
    14761 atcagcagcg tcgacagagc gatcgcgaag caaccatcgg cgtcacggac agcctggtac
    14821 atctttttgc caaccgttgg gcaccgctgg ttgtcgggcg caacatcgga ctgatgacga
    14881 tggaattatt caccccggca cgcgatgtgc tggcgcagcg caccctcggt tgggtggcgc
    14941 gttgaaggcg gttttaagga ataaacaatg caaagtgttg atgtagccat tgttggtggc
    15001 ggcatggtgg ggctggcggt tgcctgcggc ttacagggga gcggcttacg cgttgccgta
    15061 ctggagcagc gcgtaccgga acctctggcg gcggatgcac caccacaact gcgcgtttcg
    15121 gctatcaatg ccgccagcga aaaattactc acccgtcttg gcgtctggca ggacattctc
    15181 tctcgtaggg ccagttgtta tcacggtatg gaagtgtggg acaaagacag ttttggtcac
    15241 atttcgtttg acgatcaaag catgggctat agccatcttg ggcatatcgt tgaaaactca
    15301 gtgattcact acgcgctgtg gaacaaagcg cagcagtcgt cagatatcac tctgttggcc
    15361 cccgcagaat tacagcaggt cgcctgggga gaaaatgaaa ctttcctgac gctgaaagat
    15421 ggcagcatgt taacggcgcg tctggtgatt ggcgcggacg gtgctaattc ctggttgcgc
    15481 aacaaagcag atattccgct gactttctgg gattatcagc atcacgcgct ggtagcgacc
    15541 attcgcacgg aagaaccgca tgatgcggtg gcgcggcagg ttttccatgg cgaaggcatt
    15601 ctggcctttt taccgcttag cgatccgcat ctttgctcaa ttgtctggtc actgtcgcca
    15661 gaggaagcgc agcggatgca gcaggcaagt gaagatgaat ttaatcgcgc gttaaatatc
    15721 gcttttgata atcgcctggg cttatgcaag gttgagagcg cgcgtcaggt gttcccactg
    15781 acggggcgtt atgcgcgcca gtttgccgcg caccgtctgg tgctggtggg cgacgccgcg
    15841 cataccattc acccgctggc ggggcaggga gtaaatcttg gctttatgga tgctgcagag
    15901 ctgattgctg aactgaaacg gttgcatcgt caggggaaag acatcgggca gtacatttac
    15961 ctgcgccggt atgagcgtag ccgcaagcac agtgcggcgc tgatgctggc tggtatgcag
    16021 ggattccgcg atctgttttc cggtaccaat ccggcgaaaa aactgctgcg tgatattggt
    16081 ttgaaactgg ccgacacgct tcctggcgtt aaaccacaac ttatccgcca ggcaatggga
    16141 ttaaacgatt tgcctgaatg gctgcgttaa aaatttctct tctgctgttt atttgatacc
    16201 catcacactt tcatctcccg gttttttcgc cgggagattt tcctcatttg aaataaacta
    16261 atttcacctc cgttttcgca ttatattttc taatgtcatt attttttgat ttagtgtttt
    16321 ttgacatttt tttagctctt aatattgcct tattcaaatt agctttctaa tggcatcatc
    16381 tttatataga aactggtgtg ttttttggtt ttttattctg tcgcgatttt ggcgtttttt
    16441 aaccataagc taatgtgatg atcaatttta ccttatggtt aacagtctgt ttcggtggta
    16501 agttcaggca aaagagaacg attgcgttgg ggaccgggag tggctccgat gctgggtttc
    16561 gtggtgataa tttcaccatg aaaagttgtc agccccgctt attcaatgag gacaagatgg
    16621 cacaacagac tcctttgtac gaacaacaca cgctttgcgg cgctcgcatg gtggatttcc
    16681 acggctggat gatgccgctg cattacggtt cgcaaatcga cgaacatcat gcggtacgta
    16741 ccgatgccgg aatgtttgat gtgtcacata tgaccatcgt cgatctccgc ggcagccgca
    16801 cccgggagtt tctgcgttat ctgctggcga acgatgtggc gaagctcacc aaaagcggca
    16861 aagcccttta ctcggggatg ttgaatgcct ctggcggtgt gatagatgac ctcatcgtct
    16921 actactttac tgaagatttc ttccgcctcg ttgttaactc cgccacccgc gaaaaagacc
    16981 tctcctggat tacccaacac gctgaacctt tcggcatcga aattaccgtt cgtgatgacc
    17041 tttccatgat tgccgtgcaa gggccgaatg cgcaggcaaa agctgccaca ctgtttaatg
    17101 acgcccagcg tcaggcggtg gaagggatga aaccgttctt tggcgtgcag gcgggcgatc
    17161 tgtttattgc caccactggt tataccggtg aagcgggcta tgaaattgcg ctgcccaatg
    17221 aaaaagcggc cgatttctgg cgtgcgctgg tggaagcggg tgttaagcca tgtggcttgg
    17281 gcgcgcgtga cacgctgcgt ctggaagcgg gcatgaatct ttatggtcag gagatggacg
    17341 aaactatttc tcctttagcc gccaacatgg gctggactat cgcctgggaa ccggcagatc
    17401 gtgactttat cggtcgtgaa gccctggaag tgcagcgtga gcatggtaca gaaaaactgg
    17461 ttggtctggt gatgaccgaa aaaggcgtgc tgcgtaatga actgccggta cgctttaccg
    17521 atgcgcaggg caaccagcat gaaggcatta tcaccagcgg tactttctcc ccgacgctgg
    17581 gttacagcat tgcgctggcg cgcgtgctgg aaggtattgg cgaaacggcg attgtgcaaa
    17641 ttcgcaaccg tgaaatgtcg gttaaagtga caaaacctgt ttttgtgcgt aacggcaaag
    17701 ccgtcgcgtg atttactttt ttggagattg attgatgagc aacgtaccag cagaactgaa
    17761 atacagcaaa gaacacgaat ggctgcgtaa agaagccgac ggcacttaca ccgttggtat
    17821 taccgaacat gctcaggagc tgttaggcga tatggtgttt gttgacctgc cggaagtggg
    17881 cgcaacggtt agcgcgggcg atgactgcgc ggttgccgaa tcagtaaaag cggcgtcaga
    17941 catttatgcg ccagtaagcg gtgaaatcgt ggcggtaaac gacgcactga gcgattcccc
    18001 ggaactggtg aacagcgaac cgtatgcagg tggctggatc tttaaaatca aagccagcga
    18061 tgaaagcgaa ctggaatcac tgctggatgc gaccgcatac gaagcattgt tagaagacga
    18121 gtaacggctt tattcctctt ctgcgggaga ggatcagggt gaggaaaatt tatgcctcac
    18181 cctcactctc ttcgtaagga gagaggttca caattcactg cacgtttcag gaaccatcgc
    18241 tcatgacaca gacgttaagc cagcttgaaa acagcggcgc ttttattgaa cgccatatcg
    18301 gaccggacgc cgcgcaacag caagaaatgc tgaatgccgt tggcgcacaa tcgttaaacg
    18361 cgctgaccgg ccagattgta ccgaaagata ttcagcttgc gaccccgccg caggttggcg
    18421 caccggcgac cgaatacgcc gcactggcag aactcaaggc tattgccagt cgcaataaac
    18481 gcttcacgtc ttacatcggc atgggttaca ccgccgtgca gctaccgccg gttatcctgc
    18541 gtaacatgct ggaaaatccg ggctggtata ccgcgtacac tccgtatcaa cctgaagtct
    18601 cccagggccg ccttgaagca ctgctcaact tccagcaggt aacgctggat ttgactggac
    18661 tggatatggc ctctgcttct cttctggacg aggccaccgc tgccgccgaa gcaatggcga
    18721 tggcgaaacg cgtcagcaaa ctgaaaaatg ccaaccgctt cttcgtggct tccgatgtgc
    18781 atccgcaaac gctggatgtg gtccgtactc gtgccgaaac ctttggtttt gaagtgattg
    18841 tcgatgacgc gcaaaaagtg ctcgaccatc aggacgtctt cggcgtgctg ttacagcagg
    18901 taggcactac cggtgaaatt cacgactaca ctgcgcttat tagcgaactg aaatcacgca
    18961 aaattgtggt cagcgttgcc gccgatatta tggcgctggt gctgttaact gcgccgggta
    19021 aacagggcgc ggatattgtt tttggttcgg cgcaacgctt cggcgtgccg atgggctacg
    19081 gtggcccaca cgcggcattc tttgcggcga aagatgaata caaacgctca atgccgggcc
    19141 gtattatcgg tgtatcgaaa gatgcagctg gcaataccgc gctgcgcatg gcgatgcaga
    19201 ctcgcgagca acatattcgt cgtgagaaag cgaactccaa catttgtact tcccaggtac
    19261 tgctggcaaa catcgccagc ctgtatgccg tttatcacgg cccggttggc ctgaaacgta
    19321 tcgctaaccg cattcaccgt ctgaccgata ttctggcggc gggcctgcaa caaaaaggtc
    19381 tgaaactgcg ccatgcgcac tatttcgaca ccttgtgtgt ggaagtggcc gacaaagcgg
    19441 gcgtactgac gcgtgccgaa gcggctgaaa tcaacctgcg tagcgatatc ctgaacgccg
    19501 tggggatcac ccttgatgaa accaccacgc gtgaaaacgt aatgcagctt ttcagcgtgc
    19561 tgctgggcga taaccacggg ctggagatcg acacgctgga caaagacgtg gctcacgaca
    19621 gccgctctat ccagcctgcg atgctgcgcg acgacgaaat cctcacccat ccggtgttta
    19681 atcgctacca cagcgaaacc gaaatgatgc gctatatgca ctcgctggag cgtaaagatc
    19741 tggcgctgaa tcaggcgatg atcccgctgg gttcctgcac catgaaactg aacgccgccg
    19801 ccgagatgat cccaatcacc tggccggaat ttgccgaact gcacccgttt tgcccgccgg
    19861 aacaggctga aggttatcag cagatgattg cgcagctggc tgactggctg gtgaaactga
    19921 ccggttacga cgccgtttgt atgcagccga actccggcgc acagggcgaa tacgcgggcc
    19981 tgctggcgat tcgtcattat catgaaagcc gcaacgaagg gcatcgcgat atctgcctga
    20041 tcccggcttc tgcgcacgga actaaccccg cttctgcaca tatggcagga atgcaggtgg
    20101 tggttgtggc gtgtgataaa aacggcaaca tcgatctgac tgatctgcgc gcgaaagcgg
    20161 aacaggcggg cgataacctc tcctgtatca tggtgactta tccttctacc cacggcgtgt
    20221 atgaagaaac gatccgtgaa gtgtgtgaag tcgtgcatca gttcggcggt caggtttacc
    20281 ttgatggcgc gaacatgaac gcccaggttg gcatcacctc gccgggcttt attggcgcgg
    20341 acgtttcgca cctcaacctg cataaaactt tctgcattcc gcacggcggt ggtggtccgg
    20401 gtatgggacc gatcggtgtg aaagcgcatc tggcaccgtt tgtaccgggt catagcgtgg
    20461 tgcaaatcga aggcatgtta acccgtcagg gcgcagtttc tgcggcaccg ttcggtagcg
    20521 cctctatcct gccaatcagc tggatgtaca tccgcatgat gggcgcagaa gggctgaaaa
    20581 aagcaagcca ggtggcaatc ctcaacgcca actatattgc cagccgcctg caggatgcct
    20641 tcccggtgct gtataccggt cgcgacggtc gcgtggcgca cgaatgtatt ctcgatattc
    20701 gcccgctgaa agaagaaacc ggcatcagcg agctggatat tgccaagcgc ctgatcgact
    20761 acggtttcca cgcgccgacg atgtcgttcc cggtggcggg tacgctgatg gttgaaccga
    20821 ctgaatctga aagcaaagtg gaactggatt gctttatcga cgcgatgctg gctatccgcg
    20881 cagaaattga ccaggtgaaa gccgctgtct ggccgctgga agataacccg ctggtgaacg
    20941 cgccgcacat tcagagcgaa ctggtcgccg agtgggcgca tccgtacagc cgtgaagttg
    21001 cggtattccc ggcaggcgtg gcagacaaat actggccgac ggtgaaacgt ctggatgatg
    21061 tttacggcga ccgtaacctg ttctgctcct gcgtaccgat tagcgaatac cagtaattca
    21121 ctgattcgac tattttctaa aggcgcttcg gcgccttttt agtcagatga caaagtacaa
    21181 aagtgctcag acagtcccct cgcccctttg gggagagggt tagggtgagg ggaacaggcc
    21241 ggcactggcg cgaatattta ccctcacccc ggccctctcc ctgaaagggc gaggggaaaa
    21301 gcgtgccaac attgaagatt gagccagttt gttagcaatc tcaaagatac gtcaacgaat
    21361 taatttttct cggaaaaaca aatggctata gcacttgtga ccgatggcag tcgcggcatc
    21421 gggcgggcaa ctgcattact gttggcgaaa gaagagtata cggtggcggt taattatcaa
    21481 caaaatctcc atgcggcgca ggaagtggtg aacttaatca cgcaagccgg tggcaaggca
    21541 ttcgtgctcc aggcggatat cagcgacgaa aatcaggtca tcgcaatgtt tacagcaatc
    21601 gatcagcacg atgaaccgct agcagcgctg gtcaataacg ccgggatctt gtttacccag
    21661 tgcaccgttg aaaaccttac cgcagagcga atcaaccgag tactttccac caacatgacg
    21721 ggatattttc tctgctgccg cgaggcggta aaacgcatgg cgcttaaaaa tggtggcagt
    21781 ggcggcgcta tcgtcaatgt ctcttcggtg gcctcacggt tgggttcgcc aggggaatat
    21841 gttgattacg cggcatcgaa aggggcgatt gatacgttaa ccaccggact atcgctggaa
    21901 gtcgccgcgc agggggtccg cgttaactgc gtgcggccag ggtttatttt taccgaaatg
    21961 cacgccagcg gcggcgagcc tggacgcgtc gatcgcgtta agtcgaacat ccccatgcag
    22021 cgtggtggac aggcagaaga ggtcgcgcag gccattgtct ggctactaag tgataaagcc
    22081 tcttacgtca cgggaagttt tatcgatttg gcgggcggga aataaaacag ggaagttgtc
    22141 tgaccggatg caacaagtat tgcatccggt acttcatcga cttaaagctt ctcgccgttg
    22201 ctggtaatca cctctttgta ccagttaaag ctcttcttac gtgaacgcga catatcgcca
    22261 gtaccgtcgt catgtttatt cacatagata aagccgtagc gtttgctgta ctgcccggtg
    22321 gtgaacgata cgcagtcgat gcagccccac ggtgtgtagc ccatcagatc cacgccatcg
    22381 taagtcaccg cttttttcat ctcttcgata tgggcgcgca ggtagtcaat gcggtagtcg
    22441 tcgttgatgc tgccatcttc ttccactttg tcgtaagcgc caaaaccgtt ttcgacaata
    22501 aacagcggcc tctgataacg ctcatacagt tcgcaaaggg catagcgcag acctactgga
    22561 tcaatctgcc agccccagtc agacgcttta acatacgggt tcggtacgct gccttcgaaa
    22621 ccagagatcg catcgccggt gccgccttcg gccttcactg cgttggtcat gtaatagctg
    22681 aaaccaagat aatcgcaggt gccttcgcgc agtacattga gatcgccgtc ttccattttg
    22741 atgttaaatc cgcgacgctc ccactcgttc aacacatagg acgggtaata gccgcgtagc
    22801 tgcacatcgg taaagacgta gcgttcgcgc atcgactcct gagcgaacat cacatcgtcc
    22861 gggttacagc agtagggata gagcggcacc atcgccagca tacagccgac tttcatctcc
    22921 gggttaatgc gacgcgcagc tttcaccgcc agggcgctgg cgacaaactg gtgatgcagc
    22981 acctgataca tcgtctcttc cgggttctca tgctcggtat aaaccacgcc ggagcagcag
    23041 taaccgaaca gcggtgcacg ccagttacgc tggttgttaa tttcgttgaa ggtcatccag
    23101 tatttgactt tatgcttata gcgttcaaac accacttccg cgaaacggac aaagaaatca
    23161 accactttac ggttggtcca gctaccgtat tgctgcacca gatgcagcgg catttcaaag
    23221 tgggagaggg tgatcaccgg ttcgatgttg tatttcagca gttcatcgaa catatcatcg
    23281 tagaacttca gcccttcttc gtttggctga gcttcatcgc cttttggaaa aatgcgcgtc
    23341 caggctatgg aagtccgaaa acatttgaag cccatttcgg caaatagctt gatgtcttcc
    23401 ttgtagtgac cataaaaatc aacggcttca tggtttggat agtatttgcc tggcaagact
    23461 tctttggtga tttcgcgcgg cacgccgtgt gcgccaccgg tcagaacgtc acaaatgctc
    23521 ggccctttgc cgcctttgtt ccagccgcct tcgacctgat gagcggcaac tgcgccgccc
    23581 cataagaaat ctttctgtaa ggtgagtttt ttcactatca taggctccag atgagtcaat
    23641 tcttacagga aatagtacca tgcagccaaa cgacatcact ttttttcaac gtttcctgga
    23701 tgacattctg gctgggcgta aaaccatcac catccgcgac gagtctgaat cgcacttcaa
    23761 aacgggtgat gtgcttcgtg tcgggcgttt tgaagatgac ggttattttt gcacgattga
    23821 agtcaccgca acctcaaccg taacgctgga tacgctgacg gaaaaacatg cagagcagga
    23881 aaatatgacc ctgactgaac tgataaaggt cattgccgac atctatcccg gtcagacaca
    23941 gttttatgtg attgaattta aatgtcttta aattgaactc acgcctgtta gttgaaattt
    24001 cgcatttaat ttcatgattt ttaggagatt agtctcaaat cagaatttat tttagctaac
    24061 aggtgttcac tggaactatt ctcagttacg ctggagaggt atacatgtgc gtgtacgttt
    24121 ctccggagta agttatggtt cagaagcccc tcattaagca gggatattcg ctggcagagg
    24181 aaattgccaa cagcgtcagt cacggcattg ggttggtgtt tggtatcgtt gggctggtgt
    24241 tgctactggt tcaggcggtg gatctcaatg ccagcgccac agcgataacc agttacagcc
    24301 tctatggcgg cagtatgatc ctgctgttcc tcgcttcgac gctctatcac gccattcctc
    24361 atcaacgggc aaaaatgtgg ctgaagaaat ttgaccattg cgctatttac ctgttgattg
    24421 ccggaaccta cacgccgttt ttgctggtgg ggctggattc tccgttagcg cgcgggttga
    24481 tgattgttat ctggagcctg gcattgctgg gtattctgtt taaactgacc atcgcgcacc
    24541 gattcaaaat tttatctctg gtgacctatc tggcgatggg ctggctgtcg ctggtggtaa
    24601 tttatgaaat ggcagttaag ctcgcggcgg gcagcgttac cttattggcg gtaggcggcg
    24661 tggtttattc gctcggagtg attttctatg tctgcaaacg cattccctac aatcatgcca
    24721 tctggcacgg cttcgtgctc ggcggcagtg tgtgccactt tctggcgatc tatttgtata
    24781 ttgggcaggc gtaatgggta tgcctttgta ggcctgaaaa gacgcgtcag cgtcgcatca
    24841 ggcatcggag cacaactgcc ggattcggcg tgaacgcctt atccgatcta cgggcctcgt
    24901 ccctgttgtg ataaaggcat caaatcccag acatgataag acgcgtcagc gtcgcatcag
    24961 gcgataaatg cctgatgcga taaagagatt tactcttcga gcgaatacgg cagcggctca
    25021 atatgcaatg tattcgcatc gtcgcgtacg cggaagatgc tatccggttc catatcgtta
    25081 ttcatgacga cctgtaccac gacctgacca tcttccagtt ttaccgcagc cagcaccgta
    25141 ccggtacgac gccagttctc gcccattttc agctctaagt cttcaccagc ttccggcagt
    25201 cggctggcgc tacctgccag caaccagagc gcacgtttat tggcaccacg gaattttgct
    25261 cgcgccacca tctcttgtcc ggtataacag cctttcttaa agctgatacc gcccagcgcc
    25321 tggagattgg tcgcctgtgg gataaactgc ccgctgttgg cggcatcaat caccgggaaa
    25381 cccgcttcaa tgtttaatgc cagccactgt tggctattgt tcagttccgc ttcaccgcgc
    25441 agtttatcgg tcagcatatt agcagtagct tcatcggtta cgatcaggaa acgttctgcc
    25501 gggtattcaa accatagcaa agtggtcgcg ccttctttaa ctacctgttt ttctttcgaa
    25561 ggcagttcac taaagatatt tgccagcgcg gcgcgcgcct gaaaaccggc aacaccaagc
    25621 agcacatgct cgtcgtctgg cgcgatggtc actttagaga acaccgcata ttttttcagt
    25681 tcagtcagct gcggttcacg cacgctgcgc cgttcaatcc acgcaaagcc atcgccgtcg
    25741 cggaacagac gtaaattgct ccacattttg cctttggcgt cgcaatgggc ggcgagcagg
    25801 tgctgatctt ctgccatctg gctgacatct gctgtcacct gaccctgcat atatttttcg
    25861 ctgtccgcgc cagtaatggt ggcaagcgcc cagtcatcaa gcgtcatcag cgtcagcggt
    25921 aaacgggcag aagccgtagg ctgacgggga ggaaaaggtg taaaagccat aatagagtcc
    25981 tgagttgctt aacggcgtat taatgactaa tggtaaaaga gctatgaggc aatgcaagtg
    26041 ctttcactac gcgatctgtg ccttctgcga ggcgacttcc aaaaaaatga agccattgaa
    26101 ttgttgtgaa ttaatttcgt gatcgcgatg gtgtttactg atattcccgt aaaagaaggc
    26161 gaaaaacacg ttacaatagc ggggtactgg attttcgtga gacaggaaga agatggacat
    26221 taacaacaaa gcccgcattc attgggcatg ccgccgtggt atgcgcgaac tcgatatttc
    26281 aatcatgccg tttttcgaac atgagtacga cagcttaagc gatgacgaaa aacgcatctt
    26341 tattcgtctg ctggaatgtg acgatccgga cctgtttaac tggctgatga atcacggtaa
    26401 accggccgat gcagaactgg aaatgatggt ccgactcatc cagacacgga accgggaacg
    26461 tggtcctgtg gcaatctgat ttgcgcgtct cctggcgcgc acagtggctt tccttgctga
    26521 ttcatgggct ggttgccgct gttattttac tcatgccctg gccactcagt tacaccccgt
    26581 tatggatggt gttactttcg ctggtggtgt ttgattgcgt tcgcagccag cggcgtatta
    26641 atgctcgcca gggggaaatt cgcttgttga tggacgggcg tttgcgttgg caagggcagg
    26701 agtggagcat cgtcaaagca ccgtggatga ttaagagcgg catgatgctg cgtttacgtt
    26761 ctgatggcgg taaacggcaa catttatggc tggcagccga cagcatggac gaagccgaat
    26821 ggcgggattt acggcggatt ttgttgcaac aagagacgca aagataaaga tgacggcaaa
    26881 gcatatgcgc taagccgtca gtgagatcag gcgtaatgct ctgccatttc gttgaggatt
    26941 tgctcgcacc agctctgaat acgctcgtcg ctaaggtcat actggttagt ttcatccagc
    27001 gccagaccca cgaacagttg cccgtcagca atcaccggtt tcgggctggt aaattcatat
    27061 ccttccgttg gccagtagcc gacgaacttc acgcctttgg tcgagagttt gtcatgcagc
    27121 ataccgagcg catcgaggaa ccactcgccg tatcccagtt gatcgccaag cccatacagc
    27181 gcaacaattt taccttcaag gttcaggtcg tcgagctgat cccagacggc ttcccagtct
    27241 tcctggattt caccaaaatc ccaggtcggg atacccagaa tgagcacatc gtactgctcc
    27301 attaatttcg gggagtcgtc cttgaggtta tgtaaggtca ccagttctgg gccgataata
    27361 tcgcggattt tttctgccgc catttcggtg taacaggtgc tggaaccgta aaaaagaccc
    27421 atattcataa cgtaagcttc tcgattctgc tgtggctttt gcagctagtg tatcagaatc
    27481 gctttagtca cggcacaata aggcataatg gatcaaatga gtgaccataa ggggcgcaag
    27541 tgaaacagga tctggcacgc atcgagcagt ttcttgatgc tctgtggctg gaaaaaaatc
    27601 tggctgaaaa tacgttgaac gcttaccgtc gcgatctgtc aatgatggtg gagtggttgc
    27661 atcaccgcgg gttgacgctg gcgacggcgc aaagtgacga tttgcaggca ttactggcag
    27721 aacggctgga gggcgggtat aaagccacca gctcagcgcg tttgctgagt gcagtgcgcc
    27781 gattgttcca gtatctttat cgcgaaaagt ttcgtgaaga cgatcccagt gcgcatctcg
    27841 cttcaccgaa attgccccag cgtttgccaa aagatttaag cgaagcgcag gtcgaacgtt
    27901 tattacaggc gccattaatt gatcagccac tggagctacg cgataaagcc atgcttgaag
    27961 tgttgtatgc taccgggctg cgtgtctctg aactggtcgg gctgacaatg agtgatatca
    28021 gcctgcgtca gggcgtagtg cgggtcattg gtaaaggcaa caaagagcgc ctggtaccgt
    28081 taggtgaaga ggcggtttac tggctggaaa cctatctgga acatgggcgt ccgtggctgc
    28141 tgaatggtgt gtcaattgac gtgttgtttc ccagccagcg tgcgcagcag atgacgcgac
    28201 agaccttctg gcaccgcatt aaacattatg ccgtgctggc gggtatcgac agcgaaaagc
    28261 tgtcaccgca tgtgttgcgt cacgcttttg ccactcattt attaaatcat ggtgcggatt
    28321 tacgcgtggt gcagatgcta ctgggccaca gcgatctttc caccacgcaa atttatacgc
    28381 atgtcgctac cgagcgtctg cggcaacttc atcaacagca tcacccgcgg gcgtgatgtc
    28441 tgaaaagaac gggaagattt atgaagaaag gttttatgtt gtttactttg ttagcggcgt
    28501 tttcaggttt tgctcaggct gatgacgcgg caattcaaca aacgttagcc aaaatgggca
    28561 tcaaaagcag cgatattcag cccgcgcccg tagctggcat gaagacagtt ctgactaaca
    28621 gcggcgtgtt gtacatcacc gatgatggta aacatatcat tcaggggcca atgtatgacg
    28681 ttagcggcac ggctccggtc aatgtcacca ataagatgct gttaaagcag ttgaatgcgc
    28741 ttgaaaaaga gatgatcgtt tataaagcgc tgcaggaaaa acacgtcatc accgtgttta
    28801 ctgatattac ctgtggttac tgccacaaac tgcatgagca aatggcagac tataacgcgc
    28861 tggggatcac cgtgcgttat cttgctttcc cgcgccaggg gctggacagc gatgcagaga
    28921 aagaaatgaa agctatctgg tgtgcgaaag ataaaaacaa agcgtttgat gatgtgatgg
    28981 caggtaaaag cgtcgcacca gccagctgcg acgtggatat tgccgaccat tacgcacttg
    29041 gcgtccagct tggcgttagc ggtactccgg cagttgtgct gagcaatggc acacttgttc
    29101 cgggttacca gccgccgaaa gagatgaaag aattcctcga cgaacaccaa aaaatgacca
    29161 gcggtaaata attcgcgtga aacaacagat acaacttcgt cgccgtgcag tcgatgaaac
    29221 ggcagacttg cccgctgaat tgcctccctt gctgcgccgt ttatacgcca gccggggcgt
    29281 gcgcagtgcg caagaactgg aacgcagtgt taaagggatg ctgccctggc agcaactgag
    29341 cggcgtcgaa aaggccgttg agatccttta caacgccttt cgcgaaggaa cgcggattat
    29401 tgtggtcggt gatttcgacg ccgacggtgc caccagcacg gctctaagcg tgctggcgat
    29461 gcgctcgctt ggttgcagca atatcgacta cctggttcca aaccgtttcg aagacggtta
    29521 cggcttaagc ccggaagtgg tcgatcaggc ccatgcccgt ggtgcgcagt taattgtcac
    29581 ggtggataac ggtatttcct cccatgcggg ggttgaacac gctcgctcgt tgggcattcc
    29641 ggttattgtt accgatcacc atttgccggg cgaaacatta cccgcggcgg aagcgatcat
    29701 taaccctaac ttgcgcgact gtaatttccc gtcgaaatca ctggcaggcg tgggtgtggc
    29761 gttttatctg atgctggcgc tgcgcacctt tttgcgcgat cagggctggt ttgatgagcg
    29821 cggcatcgca attcctaacc tggcagaact gctggatctg gtagcgctgg ggacagtggc
    29881 ggacgtcgta ccgctggacg ctaataatcg cattctgacc tggcagggga tgagtcgcat
    29941 ccgtgccgga aagtgccgtc cggggattaa agcgctgctt gaagtggcaa accgtgatgc
    30001 acaaaaactc gccgccagcg atttaggttt tgcgctgggg ccacgtctca atgctgccgg
    30061 acgactggac gatatgtccg tcggtgtggc gctcttgctg tgcgacaaca tcggcgaagc
    30121 gcgcgtgctg gcaaatgaac tcgatgcgct aaaccagacg cgaaaagaga tcgaacaagg
    30181 aatgcaggtt gaagccctga ccctgtgcga gaaactggag cgcagccgtg acacgctacc
    30241 cggcgggctg gcaatgtatc accccgaatg gcatcagggg gttgtcggta ttctggcttc
    30301 ccgtattaaa gagcgttttc accgtccggt tatcgccttt gcgcccgcgg gtgatggtac
    30361 gctgaaaggt tctggtcgct ccattcaggg gctgcatatg cgtgatgcgc tggagcgatt
    30421 agacacactc taccctggca tgatgctgaa gtttggcggt catgcgatgg cggcgggttt
    30481 gtcgctggaa gcggataaat tcgaactctt tcaacaacgg tttggcgaac tggttactga
    30541 gtggctggac ccttcgctat tgcaaggcga agtggtgtca gacggtccgt taagcccggc
    30601 cgaaatgact atggaagtgg cgcagctgct gcgcgatgct ggcccgtggg ggcagatgtt
    30661 cccggagccg ctgtttgacg gtcatttccg gctgctgcaa cagcggctgg tgggcgaacg
    30721 tcatttgaaa gtcatggtcg aaccggtcgg cggcggtccg ctgctggatg gtattgcttt
    30781 taatgtcgat accgccctct ggccggataa cggcgtgcgc gaagtgcaac tggcttacaa
    30841 gctcgatatc aacgagtttc gcggcaaccg cagcctgcaa attatcatcg acaatatctg
    30901 gccaatttag cgtcatcttc tctataaaaa agagcgtgga ttgggtacaa tcccgctctt
    30961 atcaccgcat tttgactagc tcaataaaag aaatcagacc atgtttgaaa ttaatccggt
    31021 aaataatcgc attcaggacc tcacggaacg ctccgacgtt cttagggggt atctttgact
    31081 acgacgccaa gaaagagcgt ctggaagaag taaacgccga gctggaacag ccggatgtct
    31141 ggaatgaacc cgaacgcgca caggcgctgg gtaaagagcg ttcctccctc gaagccgttg
    31201 tcgacaccct cgaccaaatg aaacaggggc tggaagatgt ttctggtctg ctggaactgg
    31261 ctgtagaagc tgacgacgaa gaaaccttta acgaagccgt tgctgaactc gacgccctgg
    31321 aagaaaaact ggcgcagctt gagttccgcc gtatgttctc tggcgaatat gacagcgccg
    31381 actgctacct cgatattcag gcggggtctg gcggtacgga agcacaggac tgggcgagca
    31441 tgcttgagcg tatgtatctg cgctgggcag aatcgcgtgg tttcaaaact gaaatcatcg
    31501 aagagtcgga aggtgaagtg gcgggtatta aatccgtgac gatcaaaatc tccggcgatt
    31561 acgcttacgg ctggctgcgt acagaaactg gcgttcaccg cctggtgcgt aagagcccgt
    31621 ttgactccgg cggtcgtcgc cacacgtcgt tcagctctgc gtttgtttat ccggaagttg
    31681 atgatgatat tgatatcgaa atcaacccgg cggatctgcg cattgacgtt tatcgcgcgt
    31741 ccggcgcggg cggtcagcac gttaaccgta ccgaatctgc ggtgcgtatt acccacatcc
    31801 cgaccgggat cgtgacccag tgccagaacg accgttccca gcacaagaac aaagatcagg
    31861 ccatgaagca gatgaaagcg aagctttatg aactggagat gcagaagaaa aatgccgaga
    31921 aacaggcgat ggaagataac aaatctgaca tcggctgggg cagccagatt cgttcttatg
    31981 tccttgatga ctcccgcatt aaagatctgc gtaccggggt agaaactcgc aacacgcagg
    32041 ccgtgctgga cggcagcctg gatcaattta tcgaagcaag tttgaaagca gggttatgag
    32101 gaaccaacat gtctgaacaa cacgcacagg gcgctgacgc ggtagtcgat cttaacaatg
    32161 aactgaaaac gcgtcgtgag aagctggcga acctgcgcga gcaggggatt gccttcccga
    32221 acgatttccg tcgcgatcat acctctgacc aattgcacgc agaattcgac ggtaaagaga
    32281 acgaagaact ggaagcgctg aacatcgaag tcgccgttgc tggccgcatg atgacccgcc
    32341 gtattatggg taaagcgtct ttcgttaccc tgcaggatgt gggtggccgc attcagctgt
    32401 acgttgcccg tgacgatctg ccggaaggcg tttacaacga gcagttcaaa aaatgggacc
    32461 tcggcgacat cctcggcgcg aaaggtaaac tgttcaaaac caaaaccggc gaactgtcta
    32521 tccactgtac cgagctgcgt ctgctgacca aagcactgcg tccgctgccg gataaattcc
    32581 acggcttgca ggatcaggaa gcgcgctatc gtcagcgtta tctggatctc atctctaacg
    32641 atgaatcccg taacaccttt aaagtacgct cgcagatcct ctctggtatt cgccagttca
    32701 tggtgaaccg cggctttatg gaagttgaaa cgccgatgat gcaggtgatc ccaggcggtg
    32761 ccgctgcgcg tccgtttatc acccaccata acgcgctgga tctcgacatg tacctgcgta
    32821 tcgcgccgga actgtacctc aagcgtctgg tggtcggtgg cttcgagcgt gtattcgaaa
    32881 tcaaccgtaa cttccgtaac gaaggtattt ccgtacgtca taacccagag ttcaccatga
    32941 tggaactcta catggcttac gcagattaca aagatctgat cgagctgacc gaatcgctgt
    33001 tccgtactct ggcacaggat attctcggta agacggaagt gacctacggc gacgtgacgc
    33061 tggacttcgg taaaccgttc gaaaaactga ccatgcgtga agcgatcaag aaatatcgcc
    33121 cggaaaccga catggcggat ctggataact tcgactctgc gaaagcgatt gctgaatcta
    33181 tcggtatcca cgttgagaag agctggggtc tgggccgtat cgttaccgag atcttcgaag
    33241 aagtggcaga agcacatctg attcagccga ccttcattac tgaatatccg gcagaagttt
    33301 ctccgctggc gcgtcgtaac gacattaacc cggaaatcac agaccgcttt gagttcttca
    33361 tcggtggtcg tgaaatcggt aacggcttta gcgagctgaa tgacgcggaa gatcaggcgc
    33421 aacgcttcct ggatcaggtt gccgcgaaag acgcaggtga cgacgaagcg atgttctacg
    33481 atgaagatta cgttaccgca ctggaacatg gcttaccgcc gacagcaggt ctggggattg
    33541 gcatcgaccg tatggtaatg ctgttcacca acagccatac catccgcgac gttattctgt
    33601 tcccggcgat gcgtccggta aaataagcat tacgttatgc tcacaacccc ggcaaatgtc
    33661 ggggtttttt aattgtgctg cgcgaaagca gacaacaatt ttcttgcttc actattggct
    33721 gcctgcatca ccatccacgg actgaacgcc cacggtgtgg catcaatacc gtgtaataca
    33781 tctgctaaat cacaccattg ataatccatc acttcatcat cgttgatctg taacgcactg
    33841 gtggtgcgtg cggcaaatac cggacacact tcattttcca caatgccatt cggatcggtg
    33901 gcgcgatagc gaaagtcagg atagatagat tcaggaggcg taatttccac gccaagctca
    33961 taacggcaac ggcggatcac cgcgtcttcg ttgctttctc ccagttgtgg gtgcccacaa
    34021 accgagttag tccacacgcc aggccacgct tttttgctca gtgcgcggcg ggtaactaat
    34081 aattgtcctt tggcattaaa cagccaactg gagaacgcga gatgtaagcg ggtgtctgcc
    34141 gtgtgtgcgg catacttttc cagcgtaccc gtgggaactc cctgtgcatt caataaaatg
    34201 acgtgttccg tttgcataat ttctcacatg taattctgat cactcatcat tatagataat
    34261 tgaagagtga aatgattaac caagtgagcg tttatcgaca accgcccgtt ttgagcggat
    34321 gccgacaggt aaaaaccatt taaaaaagta gcatcatata tttgaagcga ctttcaatgg
    34381 ccattgcaac acagtcagaa atgacaggaa tagagagagt aaaaagcaac agcagactga
    34441 atataacgcg cattaaaaaa ttcatattat tctccatgct catttttctt cagcttgcgc
    34501 atcagaagcc acatacgtac tgtcctgact tttaaatctt tatcagagac ggattccttc
    34561 accaggtcag catgatgctg gtagccatta aagaagacat tcagcacaac ggcgctaagg
    34621 gttgcgagca taatgccgct atgcagcaac ggttgcagta cggccggtaa cttagaaaag
    34681 aaatcgtgag agagcgtcgg agtcatgcca acgccgagac tgatcgccac aatatagagg
    34741 ttgtaacggt tggtggtgta gttacagcgc gacagaattc gaatccctgt cgccagtacc
    34801 atgccgaaca tcaccagccc agcaccgccc agcacaaatt gcggaatgga ggcgaccagc
    34861 accgccattt ttggcaccat gccaaacagg attaatataa ttcccgaaga aatacacacc
    34921 cagcggctat aaatcctttc gcatagcgag taatgagcaa gataaaaatt aagacagcaa
    34981 aggagatacc taaataaacg ggattaccat attgcggatt ccctttacct ccggcggccc
    35041 agtcaatacc cacctgaatg atgctaagcc caatagaagt aataaccaca ccggtaacca
    35101 gtggcgggaa taaaggcatc aagcgaccaa taaatggcgc taataatgtg gtgataaaac
    35161 ccgcggcgat agtggcacca aatatcccca gcaggccgat atccgggttc atcccaatgg
    35221 ctatcattgg tgttacagca gcaaaggtca ccgacataat caccggcagg cggatcccca
    35281 taaagcggcc gataccgata cattgcaata atgtgacgat cccgcagcaa aagagatccg
    35341 agctaatgag catcgcaata gcatcttttg agaggcccag tcggtcacca atcataagag
    35401 gaacagcgac tgcacctgcg tacatgacaa ggacgtgttg cagaccgagt atgatcagct
    35461 ttcctggtga taatatgcgg tcaacctcat cagttgggcg gtcttgccca gaagatgagg
    35521 gaagttggga atctatggcg ctcatcagtg tttctccttt cgcccgccag agtaaaaagc
    35581 aaccgtttac gttaatgaaa ctgtctaaac acatgctcgt ggagtgccag tttttgcgag
    35641 gaaggggagt tagagacagg aattgcggag gcaatcacat aatcgtgctt aaaccacgaa
    35701 aatgacaaga gaacctgaca atggaattat ctgtaatttt ctcatattga tatttcatta
    35761 atcattctgg catgtcgcac tctagcattt aatcgttttt atctggatag cgctcttttg
    35821 atcggcaatg ttgaaataat gaaagcggca tagattgagg ggcaataatt tgaactcagt
    35881 cgcaaaatta taattaaaac ggtcatgcta cctaatatat gcccgtttat tgcatcgcga
    35941 aatttgagaa gacataactc attaaatgaa agggatgcaa atgaataagt ttatcgctgc
    36001 tgaagctgcg gaatgtatag gctgtcatgc ttgtgaaatt gcctgtgcgg tggcacacaa
    36061 tcaagaaaac tggccgctga gtcacagtga ctttcgaccg cgtatccacg ttgtagggaa
    36121 aggccaggct gcgaatccgg tggcctgcca tcactgcaac aatgcccctt gtgttacggc
    36181 ttgtccggtt aatgctctga ctttccagtc cgatagcgta caactggacg agcaaaaatg
    36241 tattggctgt aaaagatgcg caatcgcttg cccctttggc gtcgttgaga tggtcgatac
    36301 gattgcgcag aaatgcgacc tttgtaacca gcgcagttcc ggcacgcaag cctgtattga
    36361 agtctgccca acacaggcgt tacgactgat ggacgataaa gggttacagt ggataaaggt
    36421 ggctcgccag cgcaaaacgg cagcaggaaa agcgtcatca gacgctcagc catctcgcag
    36481 tgcagcgttg ctccccgtta actcgcgtaa aggcgcagat aaaatttcag cgagtgaacg
    36541 gaaaacccac tttggcgaaa tctattgcgg gctggatcca ctgcaagcga cttatgagag
    36601 tgaccgctgt gtttattgtg ccgaaaaagc taactgcaac tggcattgcc cgctgcataa
    36661 cgctattccg gattacatcc gtctggtaca ggaaggaaag attattgaag cggcagaact
    36721 ttgccaccag accagttcct taccagaaat ctgcggcagg gtatgcccac aggaccgtct
    36781 ttgtgaaggc gcatgtactt tgaaagatca ctctggcgca gtctctatcg gtaatctgga
    36841 acgctacatc accgataccg cgctggcgat gggctggcgt cccgatgtca gcaaagttgt
    36901 tccccgtatc gaaaaagtgg cggtgattgg cgctgggcca gcagggttag ggtgtgctga
    36961 tattctggcg cgcgcgggag ttcaggttga tgtctttgat cgccatccag aaattggcgg
    37021 tatgctgact tttggcattc ctcctttcaa actcgataaa acggtattaa gccagcggcg
    37081 agagatattc accgcaatgg gaatcgactt tcatcttaac tgtgaaattg gccgcgatat
    37141 tacctttagc gatttaactt ctgaatatga tgcagttttc atcggcgtgg ggacttacgg
    37201 gatgatgcga gcagatctgc cgcatgaaga tgcgcccggt gtcattcagg ctctaccgtt
    37261 cctgactgcc catacccgcc agctcatggg attgccggag tctgaagagt atccgctgac
    37321 ggacgtggaa ggtaagcgag tcgtggtatt gggcggtggc gatacgacaa tggattgttt
    37381 gcggacttcc attcgcctca atgctgccag cgtgacctgc gcgtatcgtc gtgatgaagt
    37441 cagtatgccg ggctcgcgca aagaggtggt taatgcgcgc gaggaaggtg tcgagtttca
    37501 gttcaatgtt caaccgcaat atatcgcttg tgacgaagat gggcgtttaa ctgcggtggg
    37561 cctgattcgt accgccatgg gtgagccggg gccggatggt cgccgtcgtc ctcgtccggt
    37621 agcgggttca gagtttgaat tgcccgccga tgttctgatt atggcctttg gtttccaggc
    37681 gcatgccatg ccgtggttgc agggcagcgg aattaaactc gataaatggg gcctgattca
    37741 aaccggtgac gtcgggtatt tacctaccca gacgcatctg aaaaaagtct ttgctggtgg
    37801 tgatgcagtt catggcgcgg atctggttgt cactgcaatg gccgcaggaa ggcaggcggc
    37861 gcgcgatatg ttaactctgt ttgatacgaa ggcatcgtga tgaaatcgtt aattatcgtt
    37921 aatccggctg actgtattgg ctgccgtact tgtgaagtag cctgtgtggt cgctcaccct
    37981 tcagaacagg agttgaatgc cgatgtcttt ttaccccggc tgaaggtaca gcgactggac
    38041 agcatcagtg cgccggtgat gtgccatcag tgtgaaaacg ccccttgtgt tggcgcttgc
    38101 cccgtggggg cgctgacgat gggcgagcag gtggtgcaga ccaattctgc ccgttgtatt
    38161 ggctgtcaga gttgcgtcag tgcgtgcccg tttggaatga tcaccattca gtcgttgccg
    38221 ggggataccc gacaacaaat tgtgaaatgc gatctctgtg aacagcggga agaggggcca
    38281 gcctgtgttg aatcttgccc gacacaggcg ctgcagttgc ttactgaaag agaactcagg
    38341 cgagtccgcc agcagcgtat tgttgccagc ggtgagaatc ctctgtgaaa gaaatctccg
    38401 cgctgaaagg cgcggagaat ctattagatt gcccaaccac ccgcgtaaaa tgcgaccagt
    38461 gcgacaggaa cgctaatttg ttgcccgagt accagactga atgcggtaaa tgccgtcaag
    38521 gaaatcgcac aaccaatcgc cattggcaaa ttggcctaca accccatcag caacgagccg
    38581 aagcccgcga ccagacaggt ggcaacaaac acagctgcgg gaggaaaacc tgctttgccc
    38641 agcattcccg gaacgacgat aacggaataa accatggcca gaaaggtcgt taagccagcc
    38701 agtacttcct gacgaacggt actgccacga gcggtaattt taaaataatt atccagcgcg
    38761 ccctgtggct ttggtgcgtc cggtgtttgt aggatgtctc cagacataat gatgtcctca
    38821 cgaggatgtt aaagttttat tattagttgc gttcgtacac cagacgacca tcaacgtagg
    38881 tgcggtagat cgaacggtca tcgcccaacg tcatcatcac gaacaatttg tcgactaaag
    38941 aaacagagtt gtcatagcgc agctgctgta gcggagtggc ggtgggttcc atcaccacga
    39001 aatcagcctc tttgccaggt aaaaagttgc caatcaaatc gtcaaggccc agagatttcg
    39061 ctccgccgag cgtggccagg taaaacgctt catatgccga gaggcgatag ccttgtaatt
    39121 gcaacacttt gtaggcttcg ttcagcgttt gcagcatgtt gaaagttgtt ccggcaccga
    39181 tatccgttcc catgcccact ttgactttct tctgcccaga tgccgcatgc atcgtgaatt
    39241 gcggtagcaa tcgccggagc tgcgccattt acaccgattt ccgagatcga tttcgccccg
    39301 aacgggccga ctttatcgtc gctcggcacc agcacagcgc ggaaatcacg cggaatgtca
    39361 ccaattttcg gtgcgccgta actgcgtaaa tcacgcgtta acgggtgacc ttcggcgtca
    39421 taaatgatct cttcgctcat actgtggccg atagctcgca gggtagcacc gtagatttgt
    39481 cccaatgcta attccgggtt gaccggtgta ccgcagtcca gcaaggcgta gaatttatcc
    39541 aggcggattt cacccgtacg cgtgttgacg gcaacttcag cgaagtttgc gccatacggg
    39601 aaggcgaaat caggcgtgat ataactgcca gttcccacca gtgaaccaaa gccgatgccg
    39661 gtttcgcctt tatgggcaat atccccgaaa ctaacttcgc ctttcttgcc gcgcacgacg
    39721 cccggcgttg ccagttgaac atctgccact gcctcaccta acatttgcgc gccgtggaac
    39781 agaattttct cccgtagatt ttccgctgcc aaacgcgccg cgttacccga gaagcaagta
    39841 ccggacgagg catatgcgcc tttatcaaac aacgcatgat cggtatcacc ggagataaca
    39901 tgcacgtcct gcggtgggca gtgcagcact tctgctgcca gtttcgtcac tacagtatcc
    39961 agaccagtac caatatccgc accgccagaa tgaacgataa aggtgccgtc cgattccagt
    40021 ttgatcatgc agttagcctg atcgatatcc gggatacccg atttctgcat gataatggcg
    40081 acgccgcgac cgatgtgcca gtcaccattt tgcggtttcg gtgacgacca ttggatcatc
    40141 tcgcgaccct gacgcaggat ctcttccagt gcacagctgg cggcggaagg aacggaggtc
    40201 ggcgctttac cttcaccgat tgcaccgaga attttcagct cttgcccttc gtgtacccgg
    40261 ttacgttcga taatttccag ttggtcgatc tgtaactgtt cagccagttc cgctaatgcc
    40321 atggtgatag cgaagttacc tttcggtgcg ccataaccct gataagcacc atttgggcaa
    40381 atgttgctgt agtaggtggt gacctggaaa tcgacgttat cgcacggata taacggcagc
    40441 gacagcgccg gtccgttaca cggtacggtg agtgagtggt tgccgtaagg gccagtgttg
    40501 gcgcggaaat ccatcttcac tgccgtcagg cgaccatctt ttttcgctcc cagtttgacg
    40561 gtgactttcg cgacgtgacg agaggtgtta gcaataaact cttcttcacg ggtgtagcgg
    40621 aatagtaccg gacgcccggt cacgcaggtt gcccaggcgc acacttcttc cagcaggatg
    40681 tcctgtttgg aaccaaaacc gccgccaact cgctctttaa tgacatgaac tttatgctgt
    40741 ttcatgccca cgaggcgcgc gacctggcgg cgtaagtgcc atggtacctg ggtggaggcg
    40801 tggataacca gacgatcgcc gtccatccgg gtaaagcaga tatgtgtttc agtcgggcac
    40861 tgctgtgcct gcgttgagtt ataggttcgc tcaatgatca catcggcatc ggcaaagcct
    40921 ttgtccatat cgccaatatg accatgaata ctggcggcga tatttttgcg agggcgagaa
    40981 ccgatcggga agttgatgat catatgctcg ccgcgctggg ctgcatggct gttatcgtct
    41041 tccagagtat ctggcgcacc agcaacatac accaccggtt catcgtgcac gacaggcgca
    41101 tcttccgcca ttgcttcgtc gatcgacatt accggcttaa gcacttcata ttcaacgtcg
    41161 atgagcttca gtgcttcgag cgcaatttct tcactttctg cgacgaccgc agcaacacga
    41221 tcgccgacgt gacgcatttt cttgccgaac atacggcggt caagcggtga cggttccggt
    41281 gcgctctgac cccccggggt atagtagata tcagggcaat tcaggtgagt aataacgtga
    41341 acgacgcccg gtaaggcttc agctttgctg acatccagat gagtaatcag tgcgtgagcg
    41401 tgtgggctac gtaacatttt aatgacgcag gcgtcagccg ttacgcagtc ttcaacatag
    41461 cagggtttcg cctgcaccat tttcgcggca tcagttttag gataatgctt gccaatgacg
    41521 tcaaggtcgt cacggaaagt cggagcgata tcaatggtgg cctgcggatt atttttacgt
    41581 gcaaccgcca gttcaatgac ctgatagtat tgttgccagc ctgcatcgcg gctgaacaaa
    41641 ccagaaagcg cgtcgtcgat ctcttcgcgg gtaggtgcgg cgatgcgatc gagaagatcg
    41701 gtgataatca gagctgcagc tggatcgtta taaccagact gcaccacgcc aacatcaacc
    41761 attgcctgtt gaaccagact taactcgttc catttaccca gagattctgc ggtacgaata
    41821 tctgccttct ctaactgtgc ggcaataagc aaggacgcgt taacgatatt accgttaaag
    41881 attattgcgt cagaaccggc aaacccgaag ccatcatcac tgttgcgtac agagtgcatt
    41941 cccatgttaa acaacagctt ttgcacgttt tcgcctggat taacggttag ctcctgaggc
    42001 gcgccattta aagtaaagtg gatgatcata cggcttcctc cccagcctgt tggcagtcgg
    42061 catacaggtc ggctaccaga actcccgtga tatagcgttt ataggccacg ctgccccgca
    42121 aatcttcctg cgggaaaatg gcgttggcga cagcctgttc aagtgcattg ccttccagat
    42181 tttgtttttc gacatcgtgc agacgcagtg ctttactggc gacgccatcc agcgcaattc
    42241 gcataccgtc gtggtctgtc attgcaacgg ctgccgtcac gacggttaaa cctgcctgag
    42301 aacggctaat tttgcgggtc gcacaggtgc gatacggatc tttaatgata atttcggtta
    42361 gcaggcgatc gcatgggcag gccaggtagt cctcgattga cagcgtttcg ccgttgccaa
    42421 aaaccagttc agcatccagt gctagcagga cgggaagcag cactgactct tcctgccggg
    42481 cggcgatttc accaccaatg gtcgactgat tacgaacatg gcgtgagtaa acaaaaccga
    42541 gggcttcacg cagtgctgca ggaataaagc gcgcatcacg cagtggctgc aagcgagaca
    42601 ttgcaccaat ccgcagtgca ccgttatccc agtcaaccca gtccagttcc agatcctgta
    42661 aggaaatggc aatcttttta tcggtacggg ttggtgtagc attgagtttg ctgcccccgg
    42721 cgaaccagac ggcttcatcc tggtagcggc gcttcagttc cagcgcctgt tcgacagagt
    42781 cgggcctgaa aaattgttca atcatcttct ttccttattt aaccctcttc cctgcgggaa
    42841 gagggcatct gtcatttatg ccagcgcatc catccgacgc cacatactgg cagcggcttt
    42901 tctggcttgt gcataaatgg aatcgcaatc gaagttaaac tgacggtctt catagaccat
    42961 cacaccattg accatcacgc tgtgaacgct gcctgagccc ataccgaaag cgatatgccc
    43021 ggcgatattg tctgccagca gcggcgtcgg cgagttgtaa tcgcaaatgg tcaaatcagc
    43081 tttgtaaccg gcttccagaa gcccaaattt cgcgccaaag ttgcggctca tcagttcgtt
    43141 accgttagtc agggctttgg caaaactgtc aggccacagc ggaccacccg catcgcgatg
    43201 tttaaagaag gcaaatttca tctcttcaaa catgtccgaa ccaatgccgt ccgttcccaa
    43261 tgccaggttg cggatgtcgc taaggtgatg gttgtagccg acatggttgt tcatgtttga
    43321 acgggcgtta tgcaccagga acgcatcgcg ctgattgagt agggcgatgt catctttcga
    43381 caagtacagc ccatgagcga ccagcgtttt gctgtcgatg aggtcgaatt gcgccagtcg
    43441 tgccagcagg tctttgccgt accagtggtg actgtaggaa acgtcgtaaa ggtcttccgc
    43501 agcgtgaata tgcaaaccac ggcctgtggc tttcacggct tcacgcagca tctccagacc
    43561 ggcatccggc acggtaaacg gcgcgtgagc accgatatgt gcttccacca gatacggctc
    43621 gcttgtcgct ttcttcgcct catcaatcaa acgggcgaaa cggatgtttt cttctacacc
    43681 ttcctgcaac tctttgatgc cgttgttacg gtcagtagtt tcaaaacagg tcatcgcgcg
    43741 caggccaact tttaaaaatg cgtcgcgcaa tgtggagagc gacccgccga tatacgccgg
    43801 cgaggcatgg tgatcgataa ccgatgtaca tccgctctta atcgcttcca gggaacaaat
    43861 cagtccgctg taatagagcg actcttcatc aagggcgcga tcgagccgcc accagagatt
    43921 tttcagcgtt gagatgaaat ccgggcaagg ggcgatgttt gccataattc cgcgggaaag
    43981 ctccgagtaa aaatggttgt gcgagcagac aattcccggc atcacaatcc gaccatgcat
    44041 ctctttgtag ctggcatcgg ggtagcgttg cgtcagggca tcgccgatag cgacaatcac
    44101 gtcattttcg atagcgatat caacgccttc ctgcactttc gccgggtgta actgcactgc
    44161 ggtgacattc ttcagaatca acatgattac acctccacgc ggcccagcag ataatgatga
    44221 tgctggtgga catggctgat gatgcggcac atatcgttca gctccggtgg tacgttgtta
    44281 aactgacctt cgctgtcgat gtttaacacc cagctttggt tattcagacg tactcgtacc
    44341 cggcaatctt ccacaaggaa gcctggggtg ctgctgttat caaagtcttg cgccaggctg
    44401 aagacggtga ttttgtcttc gtatggttta ccgttccacg gacagaactg agcgcagttg
    44461 ccgcattcgt tacagtaagc gtcgaggtgc agcgtctgga atctattctg gaagccaggg
    44521 actgcaatgg agacgttggc gcggttcggg cagacatcca cacacttgtt gcaaacgtag
    44581 ttacattcga ggcagcgagc ggcttcctgg gcgacaaacg cgtcacgatc gtcactgttc
    44641 accagcgtga tagagatatc gcctttacgt tgatagattt ccgctggatt gacgttgttc
    44701 cagtatttat cgttctggtg ggaacggata ttttcccgac taaggatggc atcggtcgcc
    44761 cgacgcgcgg ttccgacagc agcgacaatg gaggatggtc cgcgctgtac gtcgccgatc
    44821 ataaagacgt cagtcagacg agtttcgcca ttatggtcga cgtctggcca gccgtttttg
    44881 tccagcggca cgcccatcgc attcagggct tcagtatcct gctgttcacc aatggcggtg
    44941 atcaggctgt ctacatgcag tgttactgtt tcgttggttt caaccggacg gcgacgacct
    45001 ttctcatccg gttcgccaag cgacataacg cgcaaggtta aggtgccatc agcatcgaaa
    45061 cgttccggat tattcaggaa acggaactct acgccgtcgt gcaacgcttc ttcatactct
    45121 tcgcgccatg cgggcatctc ttgtagtgaa cgacggtaaa cgatcgttgc tttttctacg
    45181 cctggaacgc gtaacgccgc acgagcgcag tccattgcgg tgttacccgc cccgacaacg
    45241 accacatgtt tgcccagctt gagcgctgta cccttgttgt attcacgcag gaaggggagt
    45301 gatttccaga cattttggtt gtcgcccgcc agtttcacac cgctattttt atcagtgccg
    45361 gtggcaatca gaacatagtg gaagccctga ttttttaact gttcaacggt taaatcgggt
    45421 gagcagccat actcaaattt cacgccgtga gcggcaacaa aatcgatatc gtgctgaatt
    45481 aactctgcag gaatacggaa ctgaggaatg atatttttca ccacgccgcc agcattggct
    45541 tcgcgttcaa acagcgtaac cggatggccc gctctggcaa ggaagtaacc tgctgccaga
    45601 cccgccggac ctgcaccaat cacggcaacc ggatggcgtg aaccagaacc ggcgggtttg
    45661 tgccagcgtt gcttatattc atcccaacct ttttccagcg cgactttttt cagttcgcgg
    45721 atattcagcg cactgtcgta atccaggcgg gtacagttgt attggcactg gtgatcgcaa
    45781 atatgaccgg taatggcagg tagggcgttg cgttggtaga tgagttccag cgcgtcggca
    45841 tagcggtgtt cgccaagcag acggatgtat tccggaatat cttgcttgat agcgcaggca
    45901 gtaacacagg gggcaacgta gcaatcggtc agcggcaggt cttctgccac ttcaatacgc
    45961 tcttctggct tccagtgttt ctgggtgtat tccatggtta acgcgtctgc tgccagtctg
    46021 ttcagtcgtt cgacgtcaac atggtcaagt ccccaggcgt cggagccttc cagctcgcgc
    46081 atgcaggcac ttaagcgcag atagccgcca ggtttcagca ggtcggttgc catagtaata
    46141 gggcgaatac ctgtatcaaa aatatcgcgg atagtcagct gactggcacc accggaataa
    46201 gaaatgggca gtttgccgtc aaaggcgcga gagagaactg ctgcaacatt gatggagagc
    46261 gggaacagcg cacggcctga catatacatc tcttcaccag gcagtgcgcc tttattgttg
    46321 atggtgccga gagtgttagt cagttttacg ccaaagccga gtgatttttc ttttgccagt
    46381 accatcaggc gctccagcat ttccagtgct tgcgtcagct tgaggtcgtg atcaaatgac
    46441 tcttctttta agcctatgta accgaaaccg cagacatcga ggatctcacg aacacgcgcg
    46501 taccccagta aggtcgggtt aagtttgaca aaggtgttga gccctttttc ttccagcatg
    46561 tagcggcaaa tggcttcgat ttcatgcgga ggacagccgt gcatggtgga gagggtgacg
    46621 ccctgcacca tactggtggg gatgcgagcg ggtaaggctt gcaagttttc gcgtttttcc
    46681 tgcaatccgt ggcgggctaa aaatgcgtca tcctggagta atttattcag cgtatcgcga
    46741 tattgagcga atttcggatg gtcagatgcg tccatcatat tgtcgatgaa ctgttgcatc
    46801 ggcggttgct taataccttc gaggttgtaa ccgacgctca tattaaagat gaacgattta
    46861 ccggaatcag aaggctgtaa catcgcttcg agaaggtgca gggcaaacca ggctttgagg
    46921 tattcatccc aggctttaag caaggtaaat tcggtagacc attcggtgtt aaagcattcg
    46981 tcttcggcat caatacaggg cttttccagc tccaggcggt caagaatttg gacggttttt
    47041 agttcgatga agcgtccgcc agtcagccag gaagtgacaa tattttgcgc gagctgcgtg
    47101 tgcgggccag cggcagggcc gacgggagtg gcacaggttt caccgaagac gctaacagtt
    47161 ttacctttta caggtgagta aaactgttgc tcgggaatac caaagattga gcgttgttgt
    47221 tggtattcat caaatatgcg cgtcaaaagt tcctcaaacg gaatgggacg cataatatcc
    47281 cccataaccc tctccttagc ttatacagat atcattgagt gaccggtgtt gagaaatccg
    47341 gtggtttagg gggatagagc aacaatcgca ccactcttgc caggtttggc gggaaatata
    47401 aaaaaatgtg aggtggctcg aaacttaaag gctttttgtg aatgaactca caggtgccaa
    47461 atgagaatta aaatcactcc tgaaaattat tattgtcagc ccatttatca ctttttctct
    47521 tttctcaaaa tcagtgattc cttatcatta tgataaagag tgctggagag attaaatatt
    47581 tgaacgcaat cgtttaacta cattttctaa attttagtat accttttttg cgctgtaata
    47641 aagtcgtccg gagtatcaat atctaaaatt atttctgcat tttctatttc gacggaataa
    47701 tgatctccca taaggagtgc ctgacgcata ttggtgacat tgggtcgctg gattgcctgc
    47761 atcaggcatg gttttgacac taaaatcgga tggccgggga tgccattatg gagtggcagt
    47821 attgcgccat catttcgtaa cgaccagatt tttctaaaaa tatcgatggt gagggttggc
    47881 atgtcaccgt gggtgagaaa acaatgttct gtttgcactg cgggtactgc ggccttgacg
    47941 gatgttagta aaccctgcgc ataatctggg ttgtgaataa tagtgatatt gctctggttc
    48001 gcataacgtt cgtgcagttc attaccacga tagccggtga ctaaaataat tcggctacaa
    48061 aactgcaacg catttttgat acttgtatca agaattgttc cctgttccca ggggaacatc
    48121 attttccatt gccccattct tgatgataat ccggcagcgg taattataca gtcgatggct
    48181 gacatagtgg ttccattatt tataggggct aataacgcct gataattgcg ttacttcctg
    48241 atggattaag ttggggataa tttatcccag agaggtcata aagactcgtg aaaagtatag
    48301 ttgacccatc ggcattagtc attgatttag gtgcgcagaa acgtcccact gtgatttctg
    48361 ttgttggtgc tggggggaaa accagcttgc ttttttggct ggcagaactg ctccaggcta
    48421 gcggcaggcg tgtgttaatc acgacgacga cacatatgtt tatgccaacg tctcactggc
    48481 ccgtggtttt ctgtcgtgat cccgccatgc ttcctcatgc gtctcttaca tcccccattt
    48541 cattttgttt tcacagctgg aaagcgaacc agggaaaagt gcagggattt acgccagaag
    48601 cgattgatgc actggtgcaa cgaccagagt gtgacgtaat tctcattgaa gcagatggct
    48661 cgcgtggaat gccgttaaaa gcgcctgatg agcacgaacc ttgcataccc aaaagcagtt
    48721 gctgcgtgat tgctgtgatg ggaggacata ctttgggcgc gaaagtgagc acagaaaatg
    48781 tccatcgctg gtcgcagttt gctgatatta ccgggttaac acctgatgca accttgcaac
    48841 tgagcgatct cgttgcgctg gttcgccacc ctcagggggc gtttaaaaac gtaccgcaag
    48901 gttgtcggcg agtctggttc attaaccgtt tttctcaatg tgagaatgcg attgcacaaa
    48961 gcgagctcct tcaaccgctg caacaacacg acgtagaggc aatctggctg ggcgatatac
    49021 aagaacatcc tgcaatcgcg cgcagatttg tgaattagcg gcccgggtat tccgggttac
    49081 tgaaaggata tccattgagg ttggtatgaa tattttcaca gaggctgcaa aactcgaaga
    49141 gcaaaattgt ccgtttgcga tggcgcaaat tgttgatagc cgaggctcga ctccccgcca
    49201 ttctgcacaa atgttagtgc gcgccgatgg ttctatcgtc ggtacaattg gtggcggaat
    49261 ggttgagtgg aaggtgattg aagagtcact tcaggcattg caggaacgta agccgcgatt
    49321 attccatgga cgtatggctc gtaacggtgc ggatgctgtc gggtcagatt gtggaggtgc
    49381 aatgtcagta tttatcagcg tccatggtat gcgtccacgt ctggtgttga ttggcgcggg
    49441 gcatgtcaac cgggcgatag cccagagtgc ggcgctatta ggatttgata ttgccgttgc
    49501 cgatatttat cgcgaaagcc tcaatcctga actattccca ccatcaacca cgcttctcca
    49561 tgctgagtcg tttggtgcgg cagtggaaga actggatatt cgccctgata attttgtcct
    49621 gattgccacg aataatcagg atcgtgaagc cctcgacaaa ctcattgaac agcccattgc
    49681 atggttgggg ttgctggcaa gtcgccgcaa ggttcagctt ttcctgcgtc aattgcgtga
    49741 gaaaggcgtg gctgaagaac atattgcccg tttacatgcg cccgttggtt acaacatagg
    49801 tgcggaaacg ccgcaggaga tcgccatcag cgtgctggca gaaatattac gggtgaaaaa
    49861 taacgcgccg ggtgggctga tgatgaaacc ttctcatcct tccggacacc agctggtggt
    49921 gattcgcggt gcgggagata tcgccagtgg tgtggcgcta cgtctgtatc atgcgggttt
    49981 taaagtgatc atgctggaag tggaaaaacc gacagtgatt cgttgtaccg tggcgtttgc
    50041 ccaggccgtg ttcgatggcg aaatgacggt cgaaggcgtc actgctcgcc tggcaaccag
    50101 ctctgcggaa gcgatgaaac ttaccgaacg cggattcatc cctgtgatgg tagatcccgc
    50161 ctgttcattg cttgatgaac tgaaaccgct ttgcgtggtg gacgctattc tggcgaaaca
    50221 gaatttggga acgcgggcag atatggcacc agtaacaatc gcgcttgggc cgggctttac
    50281 tgcagggaag gattgtcatg cggtaattga aacaaatcgc gggcactggc tcggtcaggt
    50341 gatttactct ggttgtgcgc aggagaatac cgatgttcct ggcaatatta tggggcatac
    50401 cacccgacgg gtgatccgtg ctcctgctgc aggcattatg cgatccaacg tgaaattagg
    50461 cgatctggtg aaagagggcg atgtgattgc ctggattggt gagcatgaaa ttaaagcacc
    50521 gttgacgggg atggtgcgtg gcttgttgaa cgacggcctg gcagtggttg gtggttttaa
    50581 aattggtgat atcgatcctc gtggtgaaac cgctgatttc accagcgttt ctgataaagc
    50641 ccgggcgatt ggcggcggcg tacttgaggc gttaatgatg ttgatgcatc agggcgtgaa
    50701 agcgacaaaa gaagtgctgg aagtggctta aatgaaaatg gccggatggt attacccacc
    50761 cggccttaaa attactgaca aatcaccgtt ccggttttgc cttcaatccc ttctttcgct
    50821 ttgctcaata cggtaatcag tgcttcacga cctgcgcgag agcgagcaaa cgaggcggca
    50881 gcttccactt taggcagcat agaacctttc gcaaaatgac cttcttcgat aaagcgttca
    50941 gcatcactta atgacaggcg atcgagccac tgttcattct ctttaccgaa gttaatggcc
    51001 actttttcta ccgcagtcag aatgatcagc atatcggcat caatcatttc tgctaaacgg
    51061 gcgctggccc agtctttatc gataaccgcg ctggcaccac gcagatggtt accttcacga
    51121 ataacaggga taccaccacc gccaacggcg atcaccacct ggcctgcatc taccagagct
    51181 ttaaccgttt ctttttcaat gatatcaacc ggttttggcg aggcaacaac acggcgatag
    51241 ccgcgaccag catcttcttt cagagtgtaa ccctgttttg tcagttgctc tgcttcttgc
    51301 tcggtaaaga acgaaccgat cggcttggtc gggttgagga atgccggatc gttagcgtcg
    51361 acttcgacct gagtaaccag cgttgctaca ggtttattaa tgccccgaga aagcagttct
    51421 tctcttaacg cgttttgtag atcgtaacca atataaccct ggcttaaggc aacacagaca
    51481 gacatcggca gcattggcga gtgtgcttca gtttttgcgg cagcttcaaa ggcttggtta
    51541 atcatgccca cctgcggccc gttaccatga gtgacgataa cttcatgtcc ttgggcaatt
    51601 aaatcaacaa ttgcctgaga agtaattttt accgctttca tttgtccggc caggtcatcg
    51661 cccagcgcat tcccgcccag ggcgagaaca attttcttac tcatttttcc tcactcatca
    51721 ttcttgattg tgtatagatc cctgacagtg gttagagcac gggagggaca aacggtttgc
    51781 gtcgcaggaa acgacctcgc ccggctttgc ctgtaaaggt gccgtcacag aaaatcgttt
    51841 caccacggga taaggttctg acaatcgcgc cctgacaggt aaaaccctcc catggcgagt
    51901 agtcggcgtt gtcgtggaga tggcgatgct gaatttgttg gctctgacgt gggtcgataa
    51961 tcaccacgtc gccatcggaa ccgggcgcta ataatccttt ttgcggccac aggccaaaca
    52021 gcctggcggg cattgcgctg gttaattcaa caaagcgttc cggtgttata cgtcccgtca
    52081 tcacgccact ggaaaacagt aactgcatgc ggttttccac accgggtaag ccatttgggc
    52141 agcgactgaa atcgccttta gaaatttgca ggcgttgagc catcgagaag gtgcagtgat
    52201 cggtcgccac cacgtcaatc gcaccatcgc tgatgccaca ccacagtttg tcctgctcgc
    52261 gtacgttacg cagcggtggg ctaagaatga acttcatgcc atcttctgta tcgtaactgc
    52321 gttcgtccaa caggagatat tgtgggcagg tttcaaccca gactggctgg tgattcgcac
    52381 gggcaagacg cagataatcc agacctaagc cgttagacag gtgcacgata tagagcgggg
    52441 cgttaccggc aatttgtgcc aggttaatca tgcgggcgat ggcttccgct tcgcattcca
    52501 gagggcgact caaggcatga tagcgcggcg cggttaaccc tgcggcgata aattccgccc
    52561 gcttgctggc gatagccgca tcattttccg ggtgcacggt ggtcagcgcg ccggattcat
    52621 gcaagcggcg taatgcctgc aaaacctcgt catcgttgag tttgtattga taggttaaat
    52681 agagtttaaa actgctcagt ccttcctcga ccatcatcgg gatttcgtcg aggattgcgt
    52741 gattaatgtg ctggatcaca ccgtgaaagc tgtaatcgat gaccgcttta tgggcggcat
    52801 aaccacgata aacctccagt tgatggcgta accgacagcc gtttgggcca aatcccatat
    52861 ggtcaataat ggttgttgta ccgccacacg cagctgcgcg ggtaccggta aaaaaatcat
    52921 cacaactgcg cgcgatgccg acatcaatat tgaaatgcgt atggacatcc acgccgccag
    52981 ggaaaacgta acagccagtg gcatcaattt cttcatacgg gagctgcggc gaaatattgt
    53041 tgcccaactg gcgaacaatc ccgctttcaa tcagcaaatc ctgtttggct tgtccatctg
    53101 cgttaacgac agtgccgttt ttgatcaata cgcgcatagc aaactccaaa aaccctccgg
    53161 cgaaccggag gggaataagt gaagagtaat tactctgttg ccagccagct taacgggatt
    53221 gcagcgtaca tcgcggcaca ggtcaccagg tgagatttcc aggttttttc gttaggtgca
    53281 tgcgcttcag gttctttacc cgggccaaag ccgatcaccg gaatgccgtg acggcccatg
    53341 atagatacgc cgttagttga gaaggtccac ttatcaacaa ccggcgcttt gccaaacagc
    53401 ccttcgtagg cattcaccag tgctttaacg gtgaagtgat cttcttccac tttccaggtc
    53461 gggaagtagc attcggttgg gtaaaccagg ccagtccagg acggacggtc gtagttgtac
    53521 atagaaacaa ccgcgttagc tttctgtact gcaggcaggg cgcggatttc gtccagcgcg
    53581 ccttcccagg tttcgcccca ggtcagacgg cggtcaatag agacggcgca gctatctgct
    53641 acagcgcaac ggcttgggga tgtgaagaag atttcagaaa cggtgagggt gcctttgccg
    53701 aggaattcgt cataacccag acgttgggag agttcttgta attcgccaag aatcggaccc
    53761 attttgaaaa tggcgttgtc accgcgttct ggcgcagaac cgtggcagct aacaccctga
    53821 acatcaatac gaatttccat acgaccgcgc tgaccacggt atatctggca gtcggttggt
    53881 tcggtactga ccacaaattc cgggcgaatg ccggattgtt caataatgta ctgccagcac
    53941 agaccgtcgc agtcttcttc ctgcacagta ccggtaacca gcagggtata ttcatcttcc
    54001 agaccgaggt ctttaatgat tttaccggca taaaccatag atgccatgcc gccttcctgg
    54061 tcggaagtac cgcgaccacc gattagctca tcagtttcca tgccttcgta cggatcgaag
    54121 tcccagtttt tgatgttgcc aatgccgacg gtatcgatat gagcgtccat tgccaccaga
    54181 cgcgggccgt ggccgatata accgagaacg ttgcccatcg ggtcgatttc aactttatcg
    54241 aagccgactt tttccatctc ttctttaata cgatgtacta cgcgtttttc gtcgcagctt
    54301 tcactgggaa tagcaaccat gtcgcgcagg aagcgagtca tgtccgcctg gtaatctttt
    54361 gctttttcaa gaatcagttt gaatggaata ttcttagcca ttatttttct ccagattttt
    54421 tactccccgt tttgcaggga gtagttccaa tagggtgatt aaggtgctac agcgtgtttg
    54481 ccttcccaga caacttcgcg gtagtgcttc acgtcggtgt cgccttcagt gctgataacc
    54541 agcactacgg catctttgtc cagcgccagt ttttccatca ggctttgatg ttgcgggtga
    54601 taatgaaccg ctgcgagaac gcccaaaccg acagcgccgg attcaccgga gatgatgcgc
    54661 gggtcgttgc cgtacggatt acccagcacg cgcataccta atgcggcaac gctgtcctgg
    54721 caggagatga attgggtggc gcagttacgt aggatttccc agcccagcgg gttaggttcg
    54781 ccacaggcca ggcccgccat gatggtggcc atatcaccgc caacgttgac gatgtcgcct
    54841 ttgacgccgg agcgataaat acagtcagct ttgtcaggtt caacaataat gctgtgcaga
    54901 ttttgcgggc tgtagacgtc gaccagataa cccagcacac caccggccat tgctccgaca
    54961 ccggcttgca gcagaacgtg cgtcggggtt acgcccattt cacgcatttg ctcgacggct
    55021 tcatctgcca gggttgcgta gccttgcatg atccaggttg ggattttggt gtaaccttcc
    55081 catgccgtgt cctgtaccac ttcccagccg tgctgctgcg cgtgttgcat ggtcaggcga
    55141 acggtatcgt catagttcat atccgtgacg atgcactcgg caccgaggtt cagaatggcg
    55201 tcaacgcgtt cctgagcaga acctttcggc atgtaaatca ccgcattctg tccgagttgc
    55261 tgtgctgccc acgccacacc gcgcccgtgg ttgccgtcgg tggtggtcgc gaaagtcatt
    55321 ttttcgccga tggcattttt caggtgctca aatgacagcg tttcgatatc aagatgatat
    55381 ttttcacaca ataattgagc gatggcgtac gcaccgccaa gcattttgaa ggcgttcaga
    55441 ccgaatcgtt ttgattcgtc tttgacgaga attttcttca caccaaaaag gtttgcgaga
    55501 tcgtccagcg cacaaagcgg tgttgggcga taaccagcta ttttctgggg gaactggcgc
    55561 gccagtttgg cctggctttg cgaaaagagc ggtgatgttt cgccgttgaa aaatttgtta
    55621 tcggcgatat caatcttcaa tgagaaaacg gacatatcct atccttttgg cctctggaaa
    55681 tagagggccg accagcagat cggcccgagg ggttatttca cgcgttcttg cgcttctttc
    55741 agcagttgct cgagcagtgc acctggtttg gcgtatttac gggacaggat catcgcggcg
    55801 atgatataag gcttccagct ggcttctttg taggtagcga tacggtattt ttcgaatacg
    55861 ccttcagtca cttcaccttc tttgcaggat acgccgctga tatcagctgg caggcagtgc
    55921 atgtacaggg cttcgccatc acgggtcagt tccatcatct cttcagtaca atgccagtct
    55981 ttgtgttgcg cgttctgtgc cagacactgt ttttccagtg cttttaagcc ttcgtggtcg
    56041 ttcgcacgca gcaattcagt acgctcttcc atcactttgt aaggtgccca tgacttcgga
    56101 taaacgatgt ctgcgtcttt gaaggcttct tccatgctgg tgacctgacg gaagctacca
    56161 ccggaggctt tagcattgtt tttcgccact tcaaccacat ccgggatcag gtcgtagcct
    56221 tccggatggg ccaaggtgac atccataccg aagcgagtca tcagaccgat gatgccttgt
    56281 ggtacagaga gcggtttgcc atagcttgga gagtaggccc aggtcatggc gattttttta
    56341 cctttcaggt tttccagtga accaaagtgt tcacgtaacc acgcgaggtc agccattgac
    56401 tgagtcgggt ggtcaatatc gcattgcagg ttcactaaag ccggacgctg tggcagtaca
    56461 ccctgcttgt aaccgtcatc aagtgcagcg ccaacttcac gcatataggc gttgcctgcg
    56521 cccagataca tatcgtcgcg aataccaata gcgtcggcgc agaaggagat catattggcg
    56581 gtttcacgca cggtttcgcc gtgagcgatt tgtgatttgc cttcgtcgag atcttgttgt
    56641 gcaaggccga gcaggttaag cgcggaagca taagagaagc gggtacgggt ggagttgtcg
    56701 cggaatacgg aaatacctaa tccactatta aagactttgg ttgagatgtt ttcagcacgc
    56761 agtgctttta atgctgcggc aacgtccagt acttgtttca gttcatctgg cgtctgttcc
    56821 cacgttaaca aaaaatcttt ctcgtgaagg tgagaggtca gcgaattgat atccttaatc
    56881 agctcattaa cagttttcat catacagtcc cggtagtgga atgacaggca aagtggttta
    56941 tttcactgtg acttgccaaa gtgattggtg ttttcatagc tatacctggg actaacaata
    57001 agtgtgccag aattatccgc gcggctgcaa aagactaatt tgacggataa tgtgtggggt
    57061 tgttcacaaa ttgatgccag cttctggttg tttgtaaaat acgaaaacgt ttggcttttt
    57121 ttatcgcgtt atttatttgc tgctaagtat cagaaataag ataaaggggc aggctatctc
    57181 tcttcagttt gataaactca ccgccgtaat tctcatattg atatagaaat gtgatatctc
    57241 ttccataatt gcattagaaa atgataaaaa tcttatgttt ttatcaaagc caatttcgcc
    57301 tgagttaata tcatcatgat ctgtgaacca tcaacgtctt caatgatcga cttttaagta
    57361 agtctggaag gtaaagctat ggagcttgct actacgcagt cagtattgat gcaaattcaa
    57421 ccgacaattc agcgttttgc cagaatgctt gccagcgttt tgcagtttga ggttgagatc
    57481 gttgatgaaa acttgtgtcg cgtcgccgga acgggcgcgt atgggaagtt tcttggtcgc
    57541 cagttgagcg gcaactcacg cctgctccgc cacgtcctgg aaacgaaaac tgaaaaagtt
    57601 gtgacacagt ctcgcttcga tcccctttgc gaaggttgcg atagtaaaga aaattgccgc
    57661 gaaaaagcat ttctgggtac gcctgtcatt ttacaggatc gttgtgttgg ggtgataagt
    57721 ttgattgccg ttacccacga gcaacaagag catatcagtg ataatttacg cgaattttct
    57781 gattatgttc gccatatatc caccattttt gtttcgaaac ttctggagga tcaggggcca
    57841 ggagataaca tcagtaaaat attcgcgacc atgatcgata atatggatca gggcgtatta
    57901 gttgttgatg atgaaagtcg ggttcagttt gttaatcaga ctgccttaaa aacacttggt
    57961 gttgtacaaa ataatattat tgggaaacct atccgtttca gaccattaac atttgagagt
    58021 aattttactc atggacatat gcagcatatt gtttcgtggg acgataaaag tgaattaatc
    58081 attggtcaat tgcataacat tcagggccga caattatttt taatggcatt tcaccaatcg
    58141 cataccagtt tttctgtagc aaatgcacct gatgaaccac atattgaaca attggttggc
    58201 gagtgccgtg ttatgcggca attaaaacga ctcattagcc gtattgcacc cagcccatcc
    58261 agcgttatgg tggttggtga aagcggcacg ggtaaagaag tcgtcgcccg agcaatccat
    58321 aagttgagcg gaagacggaa taaacccttt attgctatca actgtgccgc gattccggag
    58381 cagcttctgg aaagcgaact gttcggttat gttaaaggcg catttactag tgcttctgcc
    58441 aacggtaaaa cagggttgat tcaggcggcg aatacgggca cgctgtttct cgatgaaata
    58501 ggtgatatgc cattaatgtt gcaggctaaa ttactgcgcg ctattgaggc gcgtgaaatt
    58561 ctgccgattg gtgccagtag cccaatacaa gtcgacattc gcatcatttc tgcaactaat
    58621 cagaatttgg cccagttcat tgccgaaggt aaattccgcg aagatctctt ctaccgactt
    58681 aatgttatcc cgataactct gccaccgctg cgtgaacgtc aggaagatat tgaactattg
    58741 gtgcattact ttttacatct gcatacccgt cgtctgggat cggtttatcc tggcattgct
    58801 cccgatgtcg tcgaaatatt gcgtaagcat cgttggcccg gaaacctgcg cgagttaagc
    58861 aatttgatgg aatatctggt taacgtggtt ccttcaggtg aagttatcga cagcacgcta
    58921 ttgccgccaa atctgctgaa taatggcaca acggagcaaa gtgatgtaac agaggtcagt
    58981 gaggcgcacc tgtcactcga tgatgcgggc ggcacggcgc tggaggagat ggaaaagcaa
    59041 atgatccgcg aggcgctttc acgtcataac agcaagaagc aagttgctga tgaactgggc
    59101 atcggcattg ctacgctcta tcgcaagatt aagaaatatg agttgttaaa cacataacct
    59161 cagtgaatcc ggcctgaatt caggccggat atccttttac ttcgttttct cgcaatccag
    59221 aactgtattt acaatcatct gataccccgt gcagcgacaa agatttcccg ccagtccgcg
    59281 acgaatttcc gtaatggtta atggcttctc gcgtggtttc gccagcattg ccgtggtagc
    59341 cataatcagg ccaggcgtac aaaacccgca ctgcactgcg ccggatttcg cataagcctg
    59401 ctgaacatga gaaagttttc cgcctttcgc ttcaccttcc agcgtgcgga tctcctttcc
    59461 ttcagcccag gcggcaaggt ataagcaact gtctattgct gtgccgtcga ccaacaccgt
    59521 acaggcacca cattcaccca cgcagcaccc ttgtttgaca cttagcagtc cttgttcgcg
    59581 gagtaattcc gagagcggcg tgcctggtgc ggcgtgaagc tgaaaaggca tcccgttaat
    59641 ggtgcattcg atggtaattg tttcgctgtg attcattgca attttccccc cgccgcggcg
    59701 acggcttcgc taatcacttt tttggtcatc gtctggatga gatgcagacg aaactcttta
    59761 ctggcccgcc atgaagaacg cggggcgaca tcttgcagga cagattcgct gatagcttcc
    59821 agcgtttgca ggtttaatgg cgcattttgt gcagtctgtt cggcatgttg gcagcgaatc
    59881 ggcgttggcg cggcaacacc aaatgccagg cgtaattcgc tgaaattgcc gttatccagt
    59941 cggcaatgtg cggcgcagcc aatcgttgaa atatccattg cgtcgcgcat ggcatattta
    60001 aaatgcgcgc tgcccgcgtg ttctttcggc tgtggcggaa aatgaaaggc gacgaggatt
    60061 tcgtcatgct caagagacac tttgcccggc ccggtgtgaa agccattaat cgggacgaaa
    60121 cgaacaccgc gcggggagtg gatctccagt ttcgcgtcat aaattagcgt tggcgtggca
    60181 gaatctgcgc tggtggcacc gttgcaaata tttccaccgt aggtagcgac gttacggatc
    60241 tgcggtccag caatggacgt ggccgcagca cataacgccg ggagatgacg ttgagttata
    60301 ggagcttcta ttagctgggt aaatgtcgtt gcagagccga tacgtagcga gccatcttcc
    60361 gccagcgtaa ttccccgcag ctccgccaga ttatgaatat caacaatatg gcgataacgg
    60421 tcattgtgat ggtggagctg aatcagtacg tcagtgccat cggcgagcag tttggcctgc
    60481 gggttgtcag ccagcaggtt gatggcatcg gcaagggttg ctgcgcgatg gtaagaagca
    60541 aaatcaaaca tgatgttatc ctcaaatcaa tcctgccaga tggaactctt catataaccg
    60601 ttttggcgtc agcggcagtg tattgattgc aacaccggta gccatcttca ccgcgttacg
    60661 aatagcagcg gcaacaggaa ttattggtgg ctcacccagt gacttatgtc cgtatgcgga
    60721 ttgcggctca ttgatttcga cgaacgcgct ttccagttgt ggcagatccg gcatggtcgg
    60781 cattttgtaa tccagcagat tggggttacg gaccacgccg cttttagcat cgatgatcat
    60841 ctcttcaaat agcgcccagc caatgcccat tcccattccg ccgtgtacct gaccttctgc
    60901 cagcagtgga ttaagaatat gccctgaatc atgaacgttg aggatgcggt tgatggtgac
    60961 tttgcacagc gcaatatcga ccgtcagatc aacaaaggta cagccaaacg ccgatgggtt
    61021 agtggtggtt ttgatggagc tttcagcaga gagctgcccg ccgcgttcag ggtggtagaa
    61081 agcgtccatc gccaaatctt ttaacgacat taacggctct tccggtcgtt caaccagcac
    61141 gatatggcct tttatcaggg tcagattcat cgctgactga tgtagcatga ctgcggcgtg
    61201 agcgatgatt ttctctttta ataatagtgc cgcactgcgc agcgcaggcg cggcaacata
    61261 gctctggcgt gaggcaaatg cgccgggagc gaacggcgta acgtcggtat cttgagttga
    61321 aataacgcga acgtcgctga ccggaacccc cacggtttct gccaccattt gcgagaagac
    61381 ggtgtcggca ccctgaccga tttccgtcgc gccgctttgc acgttgatgg ttccatcctg
    61441 attcatcaga aggcgcgcgc ctgctatttc tacgccgaca ggccaggtgt tagaggtata
    61501 gctaaaacag gcgacgccaa cgccgcggcg caaattgcct tgctggttct ggcattctgc
    61561 acggcgtttt tcccattcaa agattttccg gcctttttca agacactccg gcaaccctgc
    61621 gctgtaaata cgtttgcccg tgagcggatt agcatctcct tcgcgggcgg cgttgcgtaa
    61681 acgaatttca acaggatcaa tacctaacgc tgtcgcggcg tcatcaagca tagactcaac
    61741 ggcaaatacg acttgtggcg cgccataacc acgcatcgca ccagccgagg ggaggttggt
    61801 atagcaggtc tttgaactgt aagcgtaggc acaacgagga taaaggtaag cgactttatt
    61861 cctccccgca gaagcgatgg agtgcccgtg agatgcataa gcgccggtgt tagacagaac
    61921 atccagacta taacctttca atgttccgtc gcggttcacg cccatttgcc cgtcaatggt
    61981 aaaagcgtgg cgggtacggg ttgcgaggaa acactcttca cggctaaggg aaactttcac
    62041 cggaatgccg ccaagcttgc tggtcaggaa tgctgccatt ggctcttcca gtacatcctg
    62101 tttattacca aaaccgccac cgacaaacgg tttgatgact cgtacgcatg accagggaat
    62161 atccagcgcc tgaccaacca cgcggcgaac aatgtgcggg atctgggtgc tggaaacgat
    62221 ggtaattcgc gagtcatcct ccatccacgc cagcgatgtt acgctttcca tgtgacaatg
    62281 ttgaataacg ggggtctgat agtgcccctg tacctggtag tcggcggcat cgattgtttg
    62341 ttggacatta cccgtcgaca tcgtgctttg tttcagtaaa ttgccaccgt tatggattgg
    62401 tgcagcgtct tctgccagcg ccgcttctgg cgtggtgata acgggtaatt cttgccactc
    62461 aatgctgacc aattgcgccg ctttttctgc cgtgagttca tcgcgggcca cgacgatggc
    62521 aacggcgtca ccatgatgac gaacatggcg agttagcagt gcgcgatcgg cggtatcgcg
    62581 cttgttttcg tcaagtgtcc aggcatgccc agctgtagcg aatggaatat caggcacatc
    62641 ttcccaggta aaaatcgcca gtacgcccgg caaacttctg gcttgttcat cattaatact
    62701 tacggcataa ccatgtgcga tagggctacg tacatatttc gcataacaca tgcccgccat
    62761 aacataatcg tcagtatatc gtgcccgccc ggtgacctta gcaatggcat cgacgcgcat
    62821 gcatgattca cccgtagcgg ttgcttcccg cgcttccata gaaatcccct cgatttgaac
    62881 atttggttct tatggcaaag atttagtcta agcaggcaag atttacgcca gaaacgacga
    62941 taagcgttag gggaggtagg aagtgtgatg acgttaaaaa aacacagata tgatacgaaa
    63001 caggactctg aataggggca gtgatacgta tcaatatgag tggcgataaa atcaatgtga
    63061 taaattggga ttattacgca tcaggatggg aaatagggta aaaagtcagg aagttcgata
    63121 cctctacagc gaatattttg ctgatgatac ttccctttgc caaatgaaat taacctcttt
    63181 ctctattacc acgtttttcc agaagcaaga gattgggtat caagaggctg gctgctatga
    63241 taaggcgtct tgttttttaa gcgaggaaag attttgagtg cgggacgcct gaataaaaaa
    63301 tctctgggta tcgtgatgtt gttatcggtt ggactgcttt tggcgggctg ttcgggtagc
    63361 aaatcatccg atacaggaac gtattccggc tccgtttaca ccgtgaaacg gggggatacg
    63421 ctatatcgta tttcgcgcac cacgggaacc agcgtaaaag aactggcgcg actgaacggc
    63481 atttcccccc cttacaccat tgaagttggt cagaaactaa aactgggtgg ggcgaaaagt
    63541 agcagtagta cacgtaaatc aaccgccaaa tcaacgacca aaaccgcatc ggttacaccg
    63601 tcatcagcgg taccgaaatc atcctggccg ccagtagggc aacgttgttg gttatggcca
    63661 acgacaggga aagttatcat gccgtattcg acagcagatg gcggcaataa agggattgat
    63721 atctcagctc cacggggtac acctatttac gccgcgggtg caggaaaggt ggtgtatgtg
    63781 ggcaaccagc tgcgtggcta cggtaatctc atcatgatta aacacagtga agattacatt
    63841 acggcttacg cccataatga cacgatgctg gtaaataatg ggcaaagcgt gaaggctggg
    63901 caaaaaatcg ccactatggg gagcacggat gcggcatctg ttcgcctgca tttccagatt
    63961 cgttaccgtg caacggcaat tgatccgcta cgttacttgc cgccgcaggg cagcaagcca
    64021 aaatgctgat ggcgaattaa tcagcagtca gcagcgcggc acttgctaag gagagcgtaa
    64081 ggtttataat gccttacgca tctcgaagcg ggcgtagttc aatggtagaa cgagagcttc
    64141 ccaagctcta tacgagggtt cgattccctt cgccgctttt gctgtatctg attttctata
    64201 ttatcttaat tcaatcgctt cagacttttc gcaatcgccg taatatttcc ccattttgtt
    64261 gtaaataaaa agcgtttacg caatgcaatc cttttgtaac aacccaggta aaacacctta
    64321 ttttcaagtt gttaaataag tggttattta cgcttttaac aaacgattga tggaatattt
    64381 tatgaaaggg aaatctgcat taactcttct gctcgctggt atattttcgt gcggcacatg
    64441 ccaggcgaca ggggcagaag tgacgagtga atctgtattt aacattctta attccacggg
    64501 ggcagcaaca gataaaagtt atttatcgtt aaatccagat aagtacccta attatcgtct
    64561 tctgattcac tcagccaaat taaaaaatga aataaagagc cactatacca aagacgaaat
    64621 tcaagggttg ttaacattaa cagaaaacac acgaaagtta acgttgacgg agaaaccctg
    64681 gggaacattt attcttgctt ctactttcga agatgacaaa acagcggcag aaacccacta
    64741 tgatgctgtc tggctgaggg atagtttatg gggctatatg gcattagtat cagatcaggg
    64801 aaatagtgta gccgcaaaaa aggttctgct caccctgtgg gattatatgt ctacgctcga
    64861 ccagattaag cggatgcagg acgtcattag caaccccaaa cgactggatg gagttcccgg
    64921 gcaaatgaat gctgtgcata tccgatttga cagcaactcc cccgtgatgg cagatgtgca
    64981 ggaagaaggc aagccgcagc tgtggaacca taaacaaaat gatgcgttag gtctttatct
    65041 tgatctcctt atacaagcaa tcgacactgg tacaattaac gctgaagatt ggcaaaaagg
    65101 tgaccgatta aaatcggttg cgctgctgat tgcttatctg gataaagcca atttctatgt
    65161 tatggaagat tccggagcat gggaagaaga tgcccgatta aacacatctt ccgttgcact
    65221 ggtgacctct ggactggaac gtttgtcgaa cttactctcg aagaaagact ctgtttttgt
    65281 ctctgattta ctacgcgaag ctaaagccaa tgaactggat gaacctcttt caacaacgag
    65341 gttgaaccac ctcatagata aaggttatga aagaataacg ttacagcttg atttaggggg
    65401 agaatcccca ggttatctgg aaaaagataa acattacaga gaagccgatg cggctttact
    65461 taatgttatt tatcctgcaa atcttgcaaa gataaacacc agaagaaaag agcaggttct
    65521 aaaaatagtt aaaaaactag caggtccgta tggtataaaa agatacgaga aggataatta
    65581 tcagtcggca aatttttggt tcaatgatat taaaaccgat accgatcaaa attcacatgc
    65641 gaaaag
//
LOCUS       DAESHI010000008        64596 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.008, whole genome shotgun sequence.
ACCESSION   DAESHI010000008 DAESHI010000000
VERSION     DAESHI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64596
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.008"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(35..1333)
                     /gene="eno"
                     /locus_tag="KW011_000529"
     CDS             complement(35..1333)
                     /gene="eno"
                     /locus_tag="KW011_000529"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBH7658958.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1421..3058)
                     /gene="pyrG"
                     /locus_tag="KW011_000530"
     CDS             complement(1421..3058)
                     /gene="pyrG"
                     /locus_tag="KW011_000530"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HBH7658959.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3286..4077)
                     /gene="mazG"
                     /locus_tag="KW011_000531"
     CDS             complement(3286..4077)
                     /gene="mazG"
                     /locus_tag="KW011_000531"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBH7658960.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4148..4483)
                     /gene="mazF"
                     /locus_tag="KW011_000532"
     CDS             complement(4148..4483)
                     /gene="mazF"
                     /locus_tag="KW011_000532"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HBH7658961.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4483..4731)
                     /gene="mazE"
                     /locus_tag="KW011_000533"
     CDS             complement(4483..4731)
                     /gene="mazE"
                     /locus_tag="KW011_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HBH7658962.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4809..7043)
                     /locus_tag="KW011_000534"
     CDS             complement(4809..7043)
                     /locus_tag="KW011_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBH7658963.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7091..8392)
                     /gene="rlmD"
                     /locus_tag="KW011_000535"
     CDS             complement(7091..8392)
                     /gene="rlmD"
                     /locus_tag="KW011_000535"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBH7658964.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8449..11205
                     /gene="barA"
                     /locus_tag="KW011_000536"
     CDS             8449..11205
                     /gene="barA"
                     /locus_tag="KW011_000536"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HBH7658965.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11437..12777)
                     /gene="gudD"
                     /locus_tag="KW011_000537"
     CDS             complement(11437..12777)
                     /gene="gudD"
                     /locus_tag="KW011_000537"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBH7658966.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12798..14138)
                     /locus_tag="KW011_000538"
     CDS             complement(12798..14138)
                     /locus_tag="KW011_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBH7658967.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14140..15492)
                     /gene="gudP"
                     /locus_tag="KW011_000539"
     CDS             complement(14140..15492)
                     /gene="gudP"
                     /locus_tag="KW011_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HBH7658968.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15927..16376)
                     /locus_tag="KW011_000540"
     CDS             complement(15927..16376)
                     /locus_tag="KW011_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBH7658969.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16394..17176)
                     /gene="truC"
                     /locus_tag="KW011_000541"
     CDS             complement(16394..17176)
                     /gene="truC"
                     /locus_tag="KW011_000541"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HBH7658970.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17176..17505)
                     /locus_tag="KW011_000542"
     CDS             complement(17176..17505)
                     /locus_tag="KW011_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HBH7658971.1"
                     /translation="MTTHDRVRFQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18127..18672)
                     /gene="syd"
                     /locus_tag="KW011_000543"
     CDS             complement(18127..18672)
                     /gene="syd"
                     /locus_tag="KW011_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HBH7658972.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18740..19588
                     /gene="queF"
                     /locus_tag="KW011_000544"
     CDS             18740..19588
                     /gene="queF"
                     /locus_tag="KW011_000544"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HBH7658973.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19700..21064
                     /gene="ppnN"
                     /locus_tag="KW011_000545"
                     /gene_synonym="ygdH"
     CDS             19700..21064
                     /gene="ppnN"
                     /locus_tag="KW011_000545"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HBH7658974.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21620..22909
                     /gene="sdaC"
                     /locus_tag="KW011_000546"
     CDS             21620..22909
                     /gene="sdaC"
                     /locus_tag="KW011_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HBH7658975.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22967..24334
                     /gene="sdaB"
                     /locus_tag="KW011_000547"
     CDS             22967..24334
                     /gene="sdaB"
                     /locus_tag="KW011_000547"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HBH7658976.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24446..25201
                     /gene="xni"
                     /locus_tag="KW011_000548"
     CDS             24446..25201
                     /gene="xni"
                     /locus_tag="KW011_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HBH7658977.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25306..26454)
                     /gene="fucO"
                     /locus_tag="KW011_000549"
     CDS             complement(25306..26454)
                     /gene="fucO"
                     /locus_tag="KW011_000549"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBH7658978.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(26482..27129)
                     /gene="fucA"
                     /locus_tag="KW011_000550"
     CDS             complement(26482..27129)
                     /gene="fucA"
                     /locus_tag="KW011_000550"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HBH7658979.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            27676..28992
                     /gene="fucP"
                     /locus_tag="KW011_000551"
     CDS             27676..28992
                     /gene="fucP"
                     /locus_tag="KW011_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HBH7658980.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            29025..30800
                     /gene="fucI"
                     /locus_tag="KW011_000552"
     CDS             29025..30800
                     /gene="fucI"
                     /locus_tag="KW011_000552"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HBH7658981.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALTWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGSVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            30909..32327
                     /gene="fucK"
                     /locus_tag="KW011_000553"
     CDS             30909..32327
                     /gene="fucK"
                     /locus_tag="KW011_000553"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HBH7658982.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVQEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            32329..32751
                     /gene="fucU"
                     /locus_tag="KW011_000554"
     CDS             32329..32751
                     /gene="fucU"
                     /locus_tag="KW011_000554"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HBH7658983.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            32809..33540
                     /gene="fucR"
                     /locus_tag="KW011_000555"
     CDS             32809..33540
                     /gene="fucR"
                     /locus_tag="KW011_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HBH7658984.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(33584..34684)
                     /gene="rlmM"
                     /locus_tag="KW011_000556"
     CDS             complement(33584..34684)
                     /gene="rlmM"
                     /locus_tag="KW011_000556"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HBH7658985.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(34677..35072)
                     /locus_tag="KW011_000557"
     CDS             complement(34677..35072)
                     /locus_tag="KW011_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HBH7658986.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKCKGVSHE"
     gene            complement(35091..36008)
                     /gene="gcvA"
                     /locus_tag="KW011_000558"
     CDS             complement(35091..36008)
                     /gene="gcvA"
                     /locus_tag="KW011_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HBH7658987.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(36359..36586)
                     /locus_tag="KW011_000559"
     CDS             complement(36359..36586)
                     /locus_tag="KW011_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HBH7658988.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            36778..37983
                     /gene="csdA"
                     /locus_tag="KW011_000560"
     CDS             36778..37983
                     /gene="csdA"
                     /locus_tag="KW011_000560"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HBH7658989.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            37983..38426
                     /gene="csdE"
                     /locus_tag="KW011_000561"
     CDS             37983..38426
                     /gene="csdE"
                     /locus_tag="KW011_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HBH7658990.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(38477..39283)
                     /gene="tcdA"
                     /locus_tag="KW011_000562"
     CDS             complement(38477..39283)
                     /gene="tcdA"
                     /locus_tag="KW011_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HBH7658991.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(39360..40457)
                     /gene="mltA"
                     /locus_tag="KW011_000563"
     CDS             complement(39360..40457)
                     /gene="mltA"
                     /locus_tag="KW011_000563"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HBH7658992.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            40666..40742
                     /locus_tag="KW011_000564"
     tRNA            40666..40742
                     /locus_tag="KW011_000564"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40700..40702,aa:Met,seq:cat)
     gene            complement(40817..42070)
                     /gene="amiC"
                     /locus_tag="KW011_000565"
     CDS             complement(40817..42070)
                     /gene="amiC"
                     /locus_tag="KW011_000565"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HBH7658993.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            42302..43633
                     /gene="argA"
                     /locus_tag="KW011_000566"
     CDS             42302..43633
                     /gene="argA"
                     /locus_tag="KW011_000566"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HBH7658994.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(43695..45521)
                     /gene="recD"
                     /locus_tag="KW011_000567"
     CDS             complement(43695..45521)
                     /gene="recD"
                     /locus_tag="KW011_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HBH7658995.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(45521..49063)
                     /gene="recB"
                     /locus_tag="KW011_000568"
     CDS             complement(45521..49063)
                     /gene="recB"
                     /locus_tag="KW011_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HBH7658996.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(49056..51944)
                     /gene="ptrA"
                     /locus_tag="KW011_000569"
     CDS             complement(49056..51944)
                     /gene="ptrA"
                     /locus_tag="KW011_000569"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HBH7658997.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPAMPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYPQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(52120..55488)
                     /gene="recC"
                     /locus_tag="KW011_000570"
     CDS             complement(52120..55488)
                     /gene="recC"
                     /locus_tag="KW011_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HBH7658998.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLSPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(55501..55824)
                     /locus_tag="KW011_000571"
     CDS             complement(55501..55824)
                     /locus_tag="KW011_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBH7658999.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(55809..56216)
                     /locus_tag="KW011_000572"
     CDS             complement(55809..56216)
                     /locus_tag="KW011_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HBH7659000.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(56213..56776)
                     /locus_tag="KW011_000573"
     CDS             complement(56213..56776)
                     /locus_tag="KW011_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBH7659001.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(56767..57237)
                     /locus_tag="KW011_000574"
                     /pseudo
     CDS             complement(56767..57237)
                     /locus_tag="KW011_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(57422..58216)
                     /gene="thyA"
                     /locus_tag="KW011_000575"
     CDS             complement(57422..58216)
                     /gene="thyA"
                     /locus_tag="KW011_000575"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBH7659002.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(58223..59098)
                     /gene="lgt"
                     /locus_tag="KW011_000576"
     CDS             complement(58223..59098)
                     /gene="lgt"
                     /locus_tag="KW011_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBH7659003.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(59249..61495)
                     /gene="ptsP"
                     /locus_tag="KW011_000577"
     CDS             complement(59249..61495)
                     /gene="ptsP"
                     /locus_tag="KW011_000577"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBH7659004.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(61508..62038)
                     /gene="rppH"
                     /locus_tag="KW011_000578"
     CDS             complement(61508..62038)
                     /gene="rppH"
                     /locus_tag="KW011_000578"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HBH7659005.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            62723..63412
                     /gene="mutH"
                     /locus_tag="KW011_000579"
     CDS             62723..63412
                     /gene="mutH"
                     /locus_tag="KW011_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HBH7659006.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            63481..64194
                     /locus_tag="KW011_000580"
     CDS             63481..64194
                     /locus_tag="KW011_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBH7659007.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            64332..64550
                     /gene="ygdR"
                     /locus_tag="KW011_000581"
     CDS             64332..64550
                     /gene="ygdR"
                     /locus_tag="KW011_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HBH7659008.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT        15656 a        17370 c        15825 g        15745 t
ORIGIN      
        1 gaggctggca tttttaaatc agataaagtc agtcttatgc ctggcctttg atctctttac
       61 gaccgttgta cggtgctttt tcgcccagag cttcttcgat acgaatcagc tggttgtatt
      121 tagcaacacg gtcagaacgg ctcatagaac cagttttgat ctggcctgca gcagtaccaa
      181 cagccaggtc agcgatggta gcgtcttcag tttcgccaga acggtgagag ataactgcag
      241 tgtagccagc atctttcgcc atcttgattg cagccagagt ttcggtcaga gaaccgatct
      301 ggttgaattt gatcaggatg gagttagcga tacctttttc gataccttct ttcaggatct
      361 tggtgttggt tacgaacagg tcgtcaccaa ccagctggat tttgtcgccc agaactttgg
      421 tctggtatgc gaaaccgtcc cagtcagatt cgtccagacc gtcttcgata gatacgatcg
      481 gatactgttt ggtcagttct tccaggaagt gagtgaattc ttcagaggtg aaagctttgt
      541 tgccttcgcc agccagaacg tatttaccgt ctttgtagaa ttcagaagct gcgcagtcca
      601 tcgccagagt gatgtctttg cccagttcat aaccagcagc tttaacagct tcagcgataa
      661 cagccagagc ttcagcgttg gaacccaggt tcggcgcata gccaccttcg tcaccaactg
      721 cagtgttcat gcctttcgct ttcagaactt ttgccaggtg atggaaaact tcagaaccca
      781 tgcggatggc ttctttcaca gttttcgcgc caaccggctg aatcatgaat tcctggatat
      841 caacgttgtt gtcagcgtgc tcaccaccgt tgatgatgtt catcatcgga accggcatgg
      901 agtatttgcc cggagtgccg ttcagttcag cgatgtgctc gtacagcggc atccctttag
      961 cggcagcagc agctttggcg ttagccagag atacagccag gattgcgttc gcgccgaatt
     1021 tggatttgtt ttcggtgccg tccaggtcga tcatgatctt gtcaatgcca gcctgatctt
     1081 tagcgtcttt gccaatcagt gcctgagcga tcgggccgtt taccgcagca acagctttgg
     1141 ttacgccttt acccaggaaa cgggatttgt cgccatcgcg cagttccaga gcttcacggg
     1201 aaccagtaga agcacctgac ggagcagctg ccataccgac gaaaccaccc tccagatgta
     1261 cttcggcttc aacagtcggg ttaccacggg agtcgatgat ttcacgaccg atgattttta
     1321 cgattttgga cattaggttt tcctcaagtc actagttaaa ctgaaactcc agacaaacaa
     1381 cgcgtaccca aggtacgcgt tgccgttcta acttttttac ttacttcgcc tgacgtttct
     1441 ggaactcgct ggcagctttc acaaagcctg caaacagcgg gtgaccatca cgtggagtag
     1501 aagtaaactc cggatggaac tggcaagcca cgaaccacgg gtgattcgga acttcgatga
     1561 tctcgaccaa ctgatcatcc ccggaacggc ccgcaacgcg cagacctgca tcttcaatct
     1621 gtttcaacag catgttgttg acttcgtaac ggtgacgatg acgctcaaca attgtcggcg
     1681 cattgtacag ctggcgaacc aggctatcgt caaccaactg gcactgctgt gcgccgagac
     1741 gcatggtacc gccgagatcg ctcttctcgc tacgaacttc aacgttgccg ttttcatcgc
     1801 gccactcggt aatcagcgcc acaaccgggt acttacagtc tggcacaaat tccgtagagt
     1861 tggcgttctc catgttggca acatggcgag cgtaatcaat taacgccacc tgcataccca
     1921 ggcaaatgcc cagataagga atattgttct cacgcgcaaa acgcgcggtc gtaatcatgc
     1981 cttctacgcc acgatagccg aaaccgccag gtacgaggat tgcgtccaga cctttaagga
     2041 tttcaacgcc gcgcgtttca acatcttgtg aatcgatcag tttgatgttg acgctgacac
     2101 gattcttcag cccaccgtgt ttcagtgctt cgatcactga tttataagca tccggcagtt
     2161 caatgtactt gccgaccata ccgatggtga cttcacttac cggattcgct tcttcgaaga
     2221 taacctgttc ccattcggac agatttgctt ccgggcagtt taagctgaat cgtttacaaa
     2281 tataatcgtc cagcccctga gatttcaaca ggcccggaat tttatagatg gaatcgacgt
     2341 ctttcagaga aataaccgct ttttccggaa cattacagaa caatgcaatc ttcgcacgtt
     2401 cgttcgccgg aacagcgcga tctgaacgac aaatcaggat gtcaggctgg ataccgatgg
     2461 agagcagctc ttttacagag tgctgagtcg gtttggtttt gacttcacca gacgctgcca
     2521 tgtacggcac cagcgtcagg tgcataaaca gagtgtgctc acggccaatt tcaacagcca
     2581 tctggcgaat cgcttcgagg aacggcaagg attcgatatc acctactgta ccgccgattt
     2641 ctaccagtac tacgtcatga ccttcgccac cttccagcac gcgctctttg attgcgttag
     2701 tgatgtgcgg aataacctgc acggttgcgc cgaggtagtc accgcggcgt tctttacgca
     2761 gaacgtcaga gtagatacga cccgtggtga agttgttgcg gcggctcatt ttggtacgaa
     2821 tgaaacgctc gtagtgcccc aggtccaggt cggtttcagc gccgtcttca gtaacgaaca
     2881 cttccccgtg ttggattggg ctcatagtac ctggatcgac gttgatgtac ggatccagtt
     2941 tcatgatggt cacattgagg ccacgggctt caagaatggc tgcgagggag gctgcggcaa
     3001 tgcctttacc cagagaggat acgaccccgc cggtcacaaa aatatagttc gttgtcatgc
     3061 tgaacctgag aagttaggtt gaaaagacga tggaataacc aggacgggaa agcagtatac
     3121 ccgaacatga cctgtgccac aaactttcat tatccctcct cttcgccagc gcactattga
     3181 aatcaggagt gagaaaatag cccctttggg gtaaatgttt ttgacgcaaa tcaagcgctt
     3241 gtcatttaaa aaatgacaca aatggcgctt gaccgcgtaa ttcccttaga gatcaatttc
     3301 ctgccgtttt acctgttgcc agacttcttc cattgtttcg aggtcaacac ctgtcatttc
     3361 cagtccacgc gcggcaacaa tacgctccac ttcgcgaaaa cgacgctcga atttttcgtt
     3421 cgctttttgc aatgcgattt ctgctttcgt ccctaaatgg cgagccagat taaccgtggc
     3481 aaacagcagg tcccccattt cctcctccag tttagcctgg tcgacaacag cctgccgcgc
     3541 ctcgtacatc acctcgtcga tctcttcgta gactttatca accaccgggc caagcgtcgt
     3601 ccagtcgaag ccaacgttgg cgcaacgttt ctggattttt tgcgcccgca ttaaagccgg
     3661 taaactacga ggaatatcgt ccagcgccga atgctgcgct ttctgcgcgc gctcttcggt
     3721 tttgatttgc tcccaacggg caagcacttc actactgttt tcggcagaac tatcagcaaa
     3781 aacatgcgga tggcgacgct ctaatttatc gctaatagca gcgcaaatat cattaaagtc
     3841 aaagcgccct tcttcctgag ccatttgcgc gtaaaacacc acctggaata gcagatcgcc
     3901 cagttcaccg cgaagatcgt caaaatcttc acgggcgatg gcgtccagca cttcgtaggt
     3961 ttcttcaagg gtgtaaggcg caatggtggc aaatgtctgc tctttatccc acgggcagcc
     4021 gttttccgga tcgcgcaggc gctgcataat agtgagcaaa cggtcgattt gattcattga
     4081 attgtcctga aaattgcggg tctgtcaggt ggaaacctgt gaccagaata gaagtgagtt
     4141 agtaacacta cccaatcagt acgttaattt tggctttaat gagttgtaat tcctctgggg
     4201 caactgttcc tttcttcgtt gctcctcttg cccgccaggc gatacttttt acctgatcag
     4261 ctaacgctac gccatcacgt tcctgaccgg ataaaacaac ttcgaacgga tatccttttg
     4321 attgcgttgt acaaggaaca cacagacaca tacctgtttt gttgttgtac atgaaaggac
     4381 tcaggacaac agctggacga tgtccagctt gctcgctacc ttttgtcggg tcaaaatcaa
     4441 cccaaatcag atcgcccata tcgggtacgt atcggcttac cattaccaga cttccttatc
     4501 tttcggctct ccccagtcga tattctcgtg gaggttttcc ggcgtgatgt cgttgaccag
     4561 ttcagcaagc gtaaatacgg gctctttacg cactggctca ataattaatt tgccatccac
     4621 caggtcaatc ttcacttcat catcaatatt cagattgagc gcctgcatta acgtagccgg
     4681 gatccgcacc gccggtgaat ttccccaacg ctttacgcta ctgtggatca taaccctttc
     4741 ctcaaaccgc tatcatatgt agatacagta tatatcaatc tacattgtag atacgagcaa
     4801 atttctgcct aactcccgtg caaccgacgc gcgtcgataa catccggcac ctggttgagt
     4861 ttacccagca cgcggcccag cacctgcagg ttgtaaatct caatggtcat gtcgatggtt
     4921 gccagttgct gttttgtgtc gctacggctg gcaacgccaa gcacgttcac cttctcgttg
     4981 gcgagaatgg tcgtgatatc acgtaacaac ccactacgat cattagccac cacgcggacc
     5041 accagcgaat atccggcgga gtagctctca ccccataccg cgtcaacaat gcgttctggc
     5101 gcatgagagc gcagttccgc cagttgttcg caatcggcgc ggtgtactga aataccgcgc
     5161 ccctgggtaa tgaagccgac aatctcatct ccaggaatcg gctggcagca gcgcgcgatg
     5221 tggtgcatca ggttgccaac accttcgact accacgcgac cgttatcttt actgcggttt
     5281 tgcggcgtgt agcttttttg ctgaagttgc ttcagcgcgg cggcgtcctg ctcttcggca
     5341 ctcggcttat taaattgcga ttgcaggaag ttcaccatct gattgagacg gatatccccg
     5401 ccaccaatcg ccgccagcaa ctcgtcgaca tcattaaagt tgtaacgcgg cagcagatgt
     5461 ttttctgctt ctttcaggct gatccccaga tgttccagct cgtcgtcaag gatttgccgc
     5521 ccagccagaa tgtttttgtc acggtcctgt ttacggaacc aagcgtgaat tttcgaacgc
     5581 ccacggctgg ttgtgacgta accgaggttt gggtttaacc agtcacggct ggggttcggc
     5641 tgtttctggg tgataatttc aatctggtcg cccatctgca gctggtaggt gaacggcaca
     5701 atgcgcccgc caatttttgc cccgatgcag cggtgtccga catcactgtg gatgtggtaa
     5761 gcgaagtcca gcggcgttga tcccgcaggc aaatcaacga catcaccttt cggcgtaaag
     5821 acatacaccc ggtcgtcaaa gacctggctg cgtacttcgt cgagcatttc gccggaatca
     5881 gccatctctt cctgccacgc aatcagttta cgcagccagg caatccggtc ttcatgtccc
     5941 gaacgtgcgc cgccagctgc cgcgccctct ttatatttcc agtgcgcagc aacacccaac
     6001 tctgcatctt catgcatctg tttggtgcgg atttggatct caacggtttt gccacctggc
     6061 cccagaacca cggtatgaat agactgataa ccgtttggtt tcgggttagc gacataatcg
     6121 tcaaactcat ccggcaggtg gcgatagtga gtgtgcacta tccccagtgc ggcatagcaa
     6181 tcctgtaaac gctcggcgac aatacgtacc gcacgcacat caaacagctc atcaaaggcg
     6241 aggttctttt tctgcatttt acgccagatg ctgtagatgt gtttcggacg accatacact
     6301 tccgctttaa cgccttcagc tttcatctca gcgcgcagat gaccaacgaa ctcttcgatg
     6361 tagtgttcgc ggtcgagacg ccgttcatgc agcagtttgg caattcgttt gtattcggtt
     6421 ggatggaggt aacggaagca gtaatcttcc agttcccatt tcagttgtcc gattccgaga
     6481 cggttagcca gcggtgcgta gatgttggta cactcttttg ccgccagtac acgttcatct
     6541 tccggcgcat cttttacttc gcgcagatga gcaatacgct ccgccagttt gatgactacg
     6601 cagcgaaaat catcgaccat cgccaataac atccggcgaa cgttatcgac ctgttcggag
     6661 gaaacagaat cagtgtgcgt cgctttcagc tggcggatcg ccgccatatc acgcacgccg
     6721 tgaataaggt taacaaccga cttaccgacg ctctcacgca gcacatcttc gctgactacg
     6781 ttggcatcag ccagagggaa cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat
     6841 gtcgagagga tctccaccat ctcaacacca cgccacaata acagactggc atccggatgc
     6901 ccctgcgtct gttgcagaca atacgcccaa gtttcggcta agcactcaca cgacttctgg
     6961 ctggtaatac ccagacttgc gatccatttt tccggatcaa attcaccagc cttattgata
     7021 tgtgcacttc ttaccgcaac catcgtcctc tcctttaggg accagacctg ccgaaatcgg
     7081 caaatcgcaa ctatttaacg cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg
     7141 ggaacatatc cagcatcgcc agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc
     7201 tatcccgagc cagcgttgca gggttacagg atacataaac tatacgaata ggttccagtt
     7261 ttataatttg ctgcataaca cctgcggcac ctgctcgcgc cgggtccagc aacactttat
     7321 cgaagccgtt tttcgcccac ggctgctttg tgacatcttc ttcaagattt tcgtgataaa
     7381 acgtcacatt ctgtaagcca ttaagacgcg cattctgctg gcctttttcc accagcgccg
     7441 gaacgccttc tacacccacc acactggcag cttgtgtcgc caatggcagt gtaaagttgc
     7501 ccataccgca gaacaaatcc agtacgcgat cttcaggttg cacatccagc cattccaacg
     7561 cacgcgctac cattttttgg ttcacacccg cattgacctg aataaaatcg cgcgggctaa
     7621 aagttaagcg caacccattt gagtcatacc agggcatctc accagagacg gtttcgagta
     7681 tctcactatc gggggcgaga tacagatcca ggccttcaga atgcgaaaag cgttccagtt
     7741 tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg
     7801 ttgcctgtac cagttcaaca tgaccaagat ggcgcataac ttgtaagctg cccaggcatg
     7861 ccatgacttt gggcagcaat gcttcaagtt ggggcactaa aatggggcat tgtttaacgt
     7921 cgacaatgtc actggagccc gctttgcgaa accccatctg aagttgctgt gttttcggta
     7981 agtagttcag acttaaacgc gcgcgacggc gatagcccca gggaacatcg gcgatcactt
     8041 cagagacttc gtgtttcatt aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat
     8101 ccacgctggc gtgttgttgc tgacagccgc cgcatacgcc aaaatgagga cagcgtggcg
     8161 tttcgcgtcc cgggctatcg cttaaccggc gtacgacttt agcgcgggca tactgttttt
     8221 tatcttcagt aacagtaact tccgcgtttt cctgcggcaa taatccgggg ataaatagcg
     8281 ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc gaggtcgttg actgaaacgg
     8341 ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga
     8401 ctttctcaat ttaacagtgt gaccttaatt gtcccataac ggaactccat gaccaactac
     8461 agcctgcgcg cacgcatgat gattctgatc ctggcaccga ccgtccttat tggtttattg
     8521 ctgagtatct ttttcgttgt gcatcgctat aacgacttgc agcgtcaact ggaagatgcc
     8581 ggagccagca ttattgagcc gcttgcagtt tctactgaat atggcatgag cctgcaaaat
     8641 cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc gccattccga tattgttcgc
     8701 gcgatttcgg tttatgatga aaataaccga ctctttgtca cctccaattt tcatcttgat
     8761 ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt
     8821 gacggcgata ttatgatcct ccgcacgccg attatttctg agagttactc ccccgacgaa
     8881 tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg gatatattgc gctggagctg
     8941 gatcttaaat cggttcgctt gcagcaatat aaagagatct ttatttccag cgtgatgatg
     9001 ctgttttgta tcggtattgc gcttattttt ggctggcgct taatgcgcga tgtaaccggt
     9061 ccgattcgca acatggtgaa taccgtcgac cgcatccgtc gcgggcaact cgacagccgg
     9121 gtggaaggat ttatgctcgg cgagctggat atgctgaaaa acggtatcaa ctcgatggca
     9181 atgtcgctgg ctgcttatca cgaagagatg cagcacaata tcgaccaggc gacgtccgat
     9241 ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg agttagatct ggcgaaaaag
     9301 cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg caaatatgtc acacgagctg
     9361 cgtacaccac tgaatggtgt tattggcttt acccgcctga cgctgaaaac agaattaaca
     9421 ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg caaataattt gctggcaatt
     9481 attaatgatg ttctcgactt ctcgaaactg gaagcaggta agctgattct ggaaagtatt
     9541 ccattcccac tacgcagcac gctggatgaa gtcgttactc tgctggcaca ttcttctcac
     9601 gataaagggt tggaactgac gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc
     9661 gacccactgc gattacagca aatcatcact aacctggtgg ggaatgcaat taaattcacc
     9721 gagaatggca acattgatat tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag
     9781 attgaagtgc agattcggga taccggcatt ggtattcctg aacgcgatca atcgcgctta
     9841 ttccaggcct tccgacaggc tgatgccagt atttcccgcc gtcatggtgg caccggtctg
     9901 gggctggtga ttacacaaaa actggttaat gaaatgggcg gcgatatttc gttccatagc
     9961 cagccgaatc gcggttcaac tttctggttc cacattaatc tcgatctgaa cccgaacatt
    10021 attatcgaag ggccatccac ccagtgcctc gccggtaagc gcctggccta tgtcgaacca
    10081 aactccgcag cagcgcaatg cacgctggat attttaagtg aaacgccgct ggaagtggtt
    10141 tatagcccaa cgttctccgc gctgcctccc gcgcattacg acatgatgtt gttaggcatc
    10201 gcggtgacct tccgcgagcc gctaacaatg caacatgagc gattagcgaa agcggtatcg
    10261 atgaccgatt tcctgatgct ggcacttcct tgccatgcac aagtcaatgc agaaaaactc
    10321 aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa cacctacgcg cctgttgcct
    10381 gccctgacgg aattttgtca tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagc
    10441 aagctggcaa tgacagtcat ggcagttgat gacaaccccg ctaacctgaa acttatcggc
    10501 gcattgctgg aagatatggt gcaacatgtg gaactttgcg atagcgggca tcaggcggtt
    10561 gaacgggcga aacagatgcc gttcgatttg atcttaatgg atattcaaat gcctgacatg
    10621 gatggcattc gggcctgcga gctcatccac cagctccccc atcagcaaca aacgccggtt
    10681 atcgcggtaa cggcgcatgc aatggccggg caaaaagaga agctgcttgg cgcagggatg
    10741 agcgattatc tggcgaaacc gattgaagaa gagcgattgc ataatttgtt gttgcgctac
    10801 aagcctggca gcggtatttc ctctcgcgtc gtgacgcccg aagtcaacga aattgtggtg
    10861 aacccgaatg cgaccctcga ctggcaactg gcactacgcc aggcagcagg aaaaaccgat
    10921 ctagcgcgcg atatgctgca aatgttactc gatttcctgc ctgaagttcg caacaaagtt
    10981 gaggagcagc tggttggaga aaacccggaa ggcctggttg atttgattca taaactgcat
    11041 ggcagttgcg gctatagcgg tgtgccgcgt atgaagaatc tctgccaact gatcgaacaa
    11101 cagctacgta gcggtactaa agaagaagat ttggagccgg agctgctgga actgttggac
    11161 gagatggata atgtcgcgcg cgaagccagc aaaattctcg ggtaacggac gtcttgtcga
    11221 gtagcaatcg gctttttatg acgctggttt cagattggtg acaaagtgcc tgtgtttatg
    11281 ccggatgcgg cgttaacgcc ttatccggcc tacaaaacca attaaattca atgaattaaa
    11341 aaatgatgta gacctgatac atagcacaac aggctatttt gcgtttagca tcagtctcaa
    11401 gccggctcca gatagagccg gttttggttt tctgtcttaa cgcaccatgc acgggcgctt
    11461 gttatcgaac gtccagccag gaatcagata ctgcattccc atcgcatcgt cacgcgcgcc
    11521 aagcccgtgt ttctgataca gctcatgggc tttcatcact tgatccatat cgatttctac
    11581 acccagcccc ggtttttccg gcacctgaac cagaccgcct ttgatctcaa acggttcttt
    11641 ggtcaagcgc tgattgcctt cctgccaaat ccagtgcgta tcaatcgcgg tgattttacc
    11701 cggtgcagcg gcggcaacat gggtaaacat cgccagggaa atatcgaagt ggttgtttga
    11761 gtgtgaaccc caggtcaggc caaattcatg gcacatttgc gccacacgta ccgaaccttg
    11821 catagtccag aaatgcggat ccgccagcgg gatatcaacg gattgtaacg agagcgtatg
    11881 gcccatctgc cgccagtcgg tggcgatcat attggttgca gtcggcaggc cagtcgcgcg
    11941 acggaactct gccatcactt cacgcccgga gaaaccttgc tccgcaccat acggatcttc
    12001 tgcataagcc agcgaacctt tcaggtattt accaatttta attgcttcgt tcagcgacca
    12061 ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaag cgttgcgcca gtgccacaat
    12121 agactcggct tcttcttccc cggccagtac accgcctttc agtttgaaat cgttgaagcc
    12181 atatttttca tacgccgctt ccgctaggcg caccaccgca tccggcgtca tcgcttcttc
    12241 atgacgcagg cgataccagt cgcatgagtc atccggctgg ctttgatacg gcagaggtgt
    12301 ggctttgcga ttaccgacga agaacagata accgagcatt tcgacttcgc tacgctgttg
    12361 accatcgccc agcagcgatg ccacgtttac ccccagatgc tgccccagca gatccagcat
    12421 tgccgcttct atcccggtaa ctacatgaat agtggtacgc aaatcaaatg tctgcaaacc
    12481 gcgcccacca gcatcacgat cggcaaaagt attacgcacc agcgtcagaa cgtttttgta
    12541 ttcacccagc gttttaccta ccaccagcgg aatcgcatct tccagcgttt tacggatttt
    12601 ctcgccgccg ggaatttccc ctacgccagt gtgaccggaa ttatctttga taatcacaat
    12661 attacgcgta aagaacggtg cgtgtgcccc actcagattc atcagcatac tgtcatgacc
    12721 cgccaccggg atgacctgca tttcagtaac aacagacgtc gtaaattgag aactcatatt
    12781 tatgtccttt ttcagaatca atgacggccg aaaacgggac gtttacggtc aaaggtccag
    12841 ccggggatca ggtactgcat cggacctgcg tcgttacgcg caccgccagg cagacgttta
    12901 taggcctcat gtgccttttg tacctgttcc cagtccagtt ccacgcccag accgggcgca
    12961 tcaggaacgg caatttttcc gtttttaatc tccagcggat ttttggtcag gcgacaatcg
    13021 ccctcctgcc aaatccagtg ggtatcgata gcggtaggat tacccggtgc cgccgcgccc
    13081 acatgggtaa acatcgccag agagatatcg aaatggttat tagaatggca gccccaggtc
    13141 agcccccagt cgtcgcaaag ctgcgccaca cggactgcac cggaaagcgt ccagaagtgc
    13201 ggatcggcaa gtggaatatc taccgcattg agcatcaccg catgacccat ttcgcgccag
    13261 ttggtggcga tcatgttagt cgcgacgggc aagccggtcg cccgccgaaa ctccgccata
    13321 acttcacgac cggagaaacc ctgttctgcg ccgcatggat cttcggcata ggtaagaaca
    13381 tcattcagcc ctttgcatag agaaatggct tcatcaagta gccaggcacc gttgggatca
    13441 acggtaatcc gcgcatcagg gaagcgtttc ttcaatgcac gaacagtgtc gatttcttgc
    13501 tcgccaggta acaccccgcc cttaagtttg aaatctttaa agccatagcg atcctgtgag
    13561 gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt tctgatggcg caactgatac
    13621 cactcatggt tgcccggcgt attttccaga taaggaagat cggttttggt ccgatcaccg
    13681 atataaaaca gataaccgag gacggtaata gtctcgcgtt gcttgcctgg ccccaacagt
    13741 tcgcagaccg gaacattcag cgccttacct agcaggtcaa gcaaggcggc ttccagcgcc
    13801 gccacggcat taacgcgcaa ttcaaaagtc caggcacctt tgccgaaggt atcaaaatcg
    13861 gctgcctgat tacctttatg cacctgctga accactttat tcaggcgcgc aacttcctgg
    13921 cccagaacca tcggaatagc atcgaccagt gtttgataaa tcacctctcc gcccggcgct
    13981 tcaccaatgc cggtatgccc ggcgttatcg gtgagtacca caatattgcg ggtgaaatat
    14041 gcgttatgtg ccccaccaat attaagcaac atgctatcgt gcccggcgac cggaatgact
    14101 ttcatatcag taataacagg actggattgt gtcgtcatct tattgccccg cgacaggttt
    14161 cagctcgata cgcttgatat cgcccaccag caccaggtag ctcagtaccg cgattaaggc
    14221 atgaacacca acataaatca gcgccccatt aaaggagcca gtcgtgccaa cgatataacc
    14281 aattgcgatt ggcgtgacga tgccagaaat gttaccgaac atgttgaaca ggccaccgga
    14341 aagaccgctg atctcttttg gcgcggtatc tgccattact gcccaaccca gcgccccgat
    14401 gcccttaccg aagaaggcca gcgccataaa gccgatgatc atccactcaa cgttgacgta
    14461 gttgcagaac accatcacca tcgacagcaa catgcccatt acgatcggtg ttttacgcgc
    14521 aatgttcagc gatcccgtgc gacgcatcag ccagtcggaa ataatcccac ccagcacacc
    14581 gccgataaaa ccgcaaaccg ccggaacgga agccacaaag cccgctttca gaatcgacat
    14641 cccacgcgcc tgcaccagat aaaccggaaa ccaggtaatg aaaaagtaag tcagggcgtt
    14701 gatacagtac tgaccgatat aaacgccgat catcatccgt gacccgagca gttgtttgat
    14761 ctgcccccac ttcacgctga acggcacttt aactttggtg ttttgctgat ccatattgat
    14821 cagcgcgcca cccgcggcga tgtactccag ctctttctga tttacccccg gatgttgatt
    14881 tggctcgtgg atgactttca accagataaa gctgatgaca atccccagac cgcccataaa
    14941 gaagaagacg tgtgaccagc ccacttcatg cgtcagccag cccataatcg gcgcgaagat
    15001 caccgttgcg aagtattgag cggagttaaa aatcgacacc gccgttcccc tttcctgcgc
    15061 cggaaaccag gccgcaacaa tgcgactgtt gccggggaaa gatggcgctt cagcaagccc
    15121 gaccaggaag cgcagagtaa acagggcaac gataatgccg aatccactaa agatatcgac
    15181 gaagccttgc agcaaggtaa acatcgacca gataaagatc gaccagaagt agacgcgttt
    15241 tgaaccaaaa cgatccagca accaaccacc agggatctgc ccgataacat aagcccatga
    15301 gaaggcagag aacacatagc ccattcccac gggatcaagg ccgatatctt tggccatttc
    15361 cgaaccggca atagaaagcg tagcgcggtc gccgtagttg aaggatgtga cgataaacaa
    15421 catcaccact atccagtaac gagcattcgt gcgtttttcc acactgctcg cagcctgact
    15481 taaagaactc attgttgcac tcctgaaaat tcgcgttagc cacgctcact ctggactgcg
    15541 acatagccag gaaatcagag gtgacgtagg gtgttttttg ccgtttttat aggtcgttcg
    15601 ccgaatacgg cgcgtgttta tatctggcaa tagcagtata aaaagcgcgc catagcggct
    15661 caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc cgattttggc attagccctg
    15721 gacggtggaa tccacttcac ggaaatgaaa acaagaacaa gaaaggaagg gttaaaacga
    15781 agaaataaag aagagtatga aatggatcgc ttgactccag gcaaacgcca gtaaaaatcc
    15841 gcgctatgaa gcagttttta ctggcatttg cctgaaaaga ttcgattcag caccgctaaa
    15901 acgacattta ccgctcgctg aacatatcag gacaacagcg tgccccactg ttcgacccac
    15961 ggatttgatt ccgtttccgg ttccgggttc tcactggcgt caatcaacag catttcgcca
    16021 acccgctgag cgctctgttc ctgcaacaag gcatcgaact gtttgccgcc attgcagaaa
    16081 tttacataac tgctgtcgcc gagcgcaatc acgccataac gcagattcgg ctggaagccc
    16141 agactatctt tgattccctg aaagagcggc acaatgctat caggaaggtc gccctgcccg
    16201 gtcgtggacg taaccaccag aacatactta tcctgataag gcagccagtc gcttaattca
    16261 ggatcttcaa ataccgttgc tttgtggccc tgtgcggtca gaattgcttc tgcttcttcc
    16321 gccactaaca gcgaatttcc gtacatggtg ccgacaaaaa taccaatttc cgccataccg
    16381 ttttctccct ggattaggaa cttatctctc catcctgacc cgatggcgca ctgaactcaa
    16441 ccctttcatt ttcaggaagc agaccgcgcc agccaaattg tgataacgcc tgcatccagg
    16501 tgtcgtccag gcccgcgtga atagtcagcg gctcgccagt aaaaggatgc gtcaatgaca
    16561 actgactggc atgtagcatt aaccgctgga ggccaaaatg ctcagcaccg ctgcgattct
    16621 ggcgtaaatc gccatgtttg ctatcgccaa taatcggatg acgcaaatgg gcaagatgtc
    16681 ggcgaagctg atgtttgcgt ccggttttcg gctccagttc caccaggccg tagcgcgtgg
    16741 tcgggtaacg tccggtcgct accggcattt ctacggtcgc cagaccgcga taatgcgtca
    16801 ctgctggctg cgggccttta tcttcgcggg caaatttatc agcgattttg tccagttctt
    16861 ccaccagtgg ataatccagc accgcttctt ccatcaacca gccgcgcaca atcgcatggt
    16921 aacgtttctg gatttggtgc tgttcaaact gttgtgccag cagccgtccg gcctcgctgg
    16981 ataatcccat caacaacaca ccagaagtgg gtcggtccag acgatgagca gtaaaaacat
    17041 gctgtcctat ctggtcacgc acggtttgca tgaccactac tttctcgtcg cgatccagcc
    17101 agctacggtg aaccagccag ccggagggtt tatttaccgc aaccagccat tcatcctgat
    17161 agagtatttc cagcattagc tcgcatcatc cgcaaaaaga gcatccagtt tttccaactc
    17221 agccagaata agcgcgcgtt gcggatggtc cgtcgccagc gccatttcat aatagggtgc
    17281 aacagcaaaa gcgcccggta acggctggtt gttatttaac aaatcgtgca ttcgcgggat
    17341 cagcacccac tgcaaccact ccagtggttc catggtgtcc ataaagaacg gttgggtact
    17401 attaaattga tgcggctggg gttcatcgtt tcgccagtgc tgatgttcac gcagtaacgc
    17461 ttcaagcgtc tgcaactgga aacgaacgcg gtcatgagtg gtcataaagc aacttcaata
    17521 agaaaaactg ccgcgaagga tagcaggaat aaaaaaaggg agcactgtat tcacagtgct
    17581 cccggttcgt ttcgcagcat tccagctact tttgttgctc cctgctcatc cttgacaact
    17641 tttcctctgg ccttgcggcc aatcgttcat cctgaactat tgcttcctgc tcacaccacc
    17701 ccgatgtgat acttcatcct gaagtgtccc tggccttcct gacccaccga atcatcctga
    17761 ccggttctca ttctccatcc tggaggtgtc ctttaacgcg tcctgcgtca tcctcttcgc
    17821 ttcatccaga agcctttccc tgaaacacca tcctggtgtg tcctgcagaa gtgtcatcat
    17881 cctgatgttc acttcgttgt ctgactccct gtcgacgaag atagaatcgt ctttttcgcg
    17941 aagtcttaca aggcgcttac agacaatgcc ttaaggaaaa tttcatatga aagttaagaa
    18001 ttaacaatca acattttgat tttaaatgaa aataacgatt cttatctttt taagtattcg
    18061 cttatttcct ggcgatctcc tacagggtct gtaagagatc tctcacaacc agatggggta
    18121 tttggattac agaagaggct taagttgatt aaggaattcc gcaagatttg aggctaaatg
    18181 cgtccgttta cgtgttccca gcgtctcttt gcacacttca ccggagagat tacacaccga
    18241 gatgacctct aactcttctt ccagcgtcgc gataaatagc gtgggcggca gttttagcct
    18301 tttctgagtc accagatgac caatcagatt ttcctgcacc cgacggaagt cgtcttcact
    18361 ccaggtttgc agcaatgtca gtttgatatc gccaaactgc gcgtgcatat ccccggcaaa
    18421 ctgagtcgta taaaaggtat gaattgtggg ttgtatcaca atatcaaaag cgcgttcaac
    18481 cgcgtttaca ttttgttccc cggtaaacgg ctggggttgc cagtatacgg catcttcggt
    18541 agttgaaata atgcacggtg aaggaacacc gtacagttcc tcacttaacg gccagctttt
    18601 atgctcttca tgccacgcat cgcagtagcg tgcagtaaaa tctttcaggg cttgtgcggt
    18661 caaatcgtcc actaatttct ctcttcactt aaaccagata cacttgtcgt ttagtttatc
    18721 tggtttatga cggtgaaaca tgtcttctta tgcaaaccat caggcacttg cgggcctgac
    18781 tcttggaaaa tcaaccgatt accgggatac ctatgacgcc agcctactgc aaggcgttcc
    18841 acgcagcctg aatcgcgacc cgctgggtct gaaagcggat aacctgcctt ttcacggtac
    18901 ggatatctgg acgctgtatg aactttcctg gctgaatgcg aaaggtttgc cgcaggtcgc
    18961 tgtcggtcat gttgaacttg attacaccag cgtaaatctg attgagtcga agagttttaa
    19021 gctctatctc aacagtttta accagacgcg ttttaataac tgggatgagg tgcgccagac
    19081 gctggagcgc gacttaagca cttgcgctca gggtaagatt agcgtggcgt tatatcgtct
    19141 tgatgaactg gaaggccagc cgataggtca ttttaatggc acttgcattg atgaccagga
    19201 tatcactatc gataactatg aattcactac tgactatctg gagaatgcca cctgtggtga
    19261 aaaagtagtg gaagagacgc ttgtcagcca cctgctgaaa tcaaactgcc tgatcaccca
    19321 tcaaccagat tggggttcga tccaaattca gtatcgtgga cgccaaattg acagagaaaa
    19381 actgctgcgt tacctggtct cattccgtca tcacaacgag ttccacgaac agtgcgtgga
    19441 acgcatcttt aatgacctgt tacgcttctg ccagccagaa aaattgagcg tttacgcacg
    19501 ttatacccgt cgtggcggtc tggacattaa cccgtggcgc agtaatagcg attttgtccc
    19561 atcgaccaca agactggttc ggcaataaat tttttctcaa ttttgcgtgc tggattcacg
    19621 cagaaggttg tgaaaggaca tcaggcaggg ctattgtaat caaagggaat gacgatattc
    19681 gtcccataag gagtttttct tgattacaca tattagcccg cttggctcca tggatatgtt
    19741 gtcgcagctg gaagtggata tgcttaaacg caccgccagc agcgacctct atcaactgtt
    19801 tcgcaactgt tcacttgccg tactgaactc cggtagtttg accgataaca gcaaagaatt
    19861 gctgtctcgt tttgaaaatt tcgatattaa cgtcctgcgc cgtgaacgcg gcgtaaagct
    19921 ggaactgatt aatcccccgg aagaggcttt tgtcgatggg cgaattattc gcgcgttgca
    19981 ggccaacttg ttcgcggttc tgcgtgacat tctcttcgtt tacgggcaaa tccataacac
    20041 cgttcgtttt cccaacctga atctcgacaa ctccgtccac atcactaacc tggtcttttc
    20101 catcttgcgt aacgctcgcg cactgcatgt cggtgaagcg ccaaatatgg tggtctgctg
    20161 gggcggacac tcaattaacg aaaacgagta tttgtatgcc cgtcgcgtcg ggaatcagct
    20221 gggcctgcgt gagctgaata tctgcaccgg ctgtggtccg ggagcgatgg aagcgccgat
    20281 gaaaggtgct gcggtcggac acgcacagca gcgttacaaa gacagtcgtt ttattggtat
    20341 gacagagccg tcgattatcg ccgctgaacc gcctaacccg ctggtcaacg aattgatcat
    20401 catgccagat atcgaaaaac gtctggaagc gtttgtccgt atcgctcacg gcatcattat
    20461 cttccctggc ggtgtgggta cggcagaaga gttgctgtat ttgctgggaa ttttaatgaa
    20521 cccggccaac aaagatcagg ttttaccatt gttcctcacc ggcccgaaag agagcgccga
    20581 ctacttccgc gtactggacg agtttgtcgt gcatacgttg ggtgaaaacg cgcgccgcca
    20641 ttaccgcatc atcattgatg acgccgctga agtcgctcgt cagatgaaaa aatcgatgcc
    20701 gctggtgaaa gaaaatcgcc gcgacacagg cgatgcctac agctttaact ggtcaatgcg
    20761 cattgcgcca gatttgcaaa tgccgtttga gccgtctcac gagaatatgg ctaatctgaa
    20821 gctttacccg gatcaacctg ttgaagtgct ggctgccgat ctgcgccgtg cgttctccgg
    20881 tattgtggcg ggtaacgtaa aagaagtcgg tattcgcgcc attgaagagt ttggtcctta
    20941 caaaatcaac ggcgataaag agattatgcg tcgtatggac gacctgctac agggttttgt
    21001 tgcccagcat cgtatgaagt tgccaggctc agcctacatc ccttgctacg aaatctgcac
    21061 gtaatctccc tcccccggtg acgtttgttg ccgggttttc tcgtttttgg tcacataccc
    21121 atcaatttat ttcatttgtt atatgaatgt ttcttaccac cctcacgcga taaatatcat
    21181 tgcagttaat atgtcataca atttatgttg taacgcaaac gtttccctat tttcataaac
    21241 cacattttat cgctatgaaa aagaaattat cgccatgatt aactaaaagt attgattttt
    21301 tcagttcaac ctacatatat tgcgcgcccc ggaagaagtc aaatgtcgtt taatgggcaa
    21361 atattgccct taaattctct tttacttttg atttacagag taaagcgttg ggataatcta
    21421 tcttccaagt agattattgt atttgggatc aagatcactg atagatacat aacttgtgtg
    21481 tatctttccg ccctcaaatt attacggcgg taaatgatta agccatcgcc gatagacaga
    21541 tttcattttt acggtcaggc accttcccga gctgaactgg ctaaaagctg aattatttgc
    21601 attcctccag gagaaataga tggaaacgac tcaaaccagc acgattgcgt cgaaagactc
    21661 tcgtagtgcc tggcgcaaga cagacaccat gtggatgctg ggcctttacg gcacggcaat
    21721 cggcgcgggc gtgctgttcc tgccaatcaa cgccggtgtt ggcggtatga tcccgctgat
    21781 catcatggct atccttgcgt tcccgatgac attttttgct caccgcggcc tgactcgctt
    21841 cgtactgtct ggtaaaaacc caggcgaaga catcaccgag gttgtagaag aacactttgg
    21901 tattggcgca ggtaaactga ttaccctgct ctacttcttc gctatctacc cgatcctgct
    21961 ggtttatagc gtggcaatca ccaatacagt tgaaagcttc atgtctcacc agctgggtat
    22021 gacaccaccg ccgcgtgcga ttctgtcgct gatcctgatc gtgggtatga tgaccatcgt
    22081 tcgcttcggt gagcagatga tcgttaaagc aatgagtatt ctggtattcc cgtttgttgg
    22141 cgtactgatg ctgctggctc tgtacctgat cccgcagtgg aacggcgcag cactggaaac
    22201 gctgtctctg gacactgcat ctgcaaccgg aaacggtctg tggatgaccc tgtggctggc
    22261 aattccggtt atggtgttct ctttcaacca ctctccgatc atctcttctt tcgccgttgc
    22321 gaagcgtgaa gagtacggcg atatggcaga acagaaatgc tcgaagatcc tggcattcgc
    22381 acacatcatg atggtgctga ccgtaatgtt cttcgtcttc agctgtgtac tgagcctgac
    22441 tccggcagac ctggctgcgg ctaaagagca gaacatctcg attctgtctt acctggctaa
    22501 ccactttaac gcaccggtta tcgcgtggat ggctccgatt atcgcgatta tcgctatcac
    22561 caaatccttc ctcggccact acctgggcgc acgtgaaggc ttcaacggta tggtgattaa
    22621 atctctgcgt ggtaaaggta agtctatcga aatcaacaag ctgaaccgta tcactgcgct
    22681 gttcatgctg gtaacgacct ggattgttgc caccctgaac ccgagcatcc tgggtatgat
    22741 tgaaaccctg ggcggcccaa tcatcgcgat gatcctgttc ctgatgccga tgtacgcaat
    22801 tcagaaagta ccggcaatgc gtaagtacag cggtcacatc agcaacgtat tcgttgtcgt
    22861 gatgggtctg attgcaatct ccgcaatctt ctactctctg ttcagctaag tcctttcgcg
    22921 ccgctttcgg gcggcgcttc ctccgtttta acgcgatgta tttcctatga ttagcgtatt
    22981 cgatattttc aaaatcggca ttggcccttc cagttctcat accgttggac caatgaaagc
    23041 gggtaaacaa tttaccgacg atctgattgc ccgtaatctg cttaaagacg tgacccgcgt
    23101 ggtggttgac gtgtacggct cgctctctct gaccggtaaa ggccaccaca ctgatatcgc
    23161 cattattatg ggcctggcgg gtaacctgcc ggataccgtg gatatcgatt ccatccctgg
    23221 ttttattcag gatgtgaata ctcatggtcg cctgatgctg gcaaacggtc agcatgaagt
    23281 ggagttcccg gttgatcagt gcatgaactt ccatgcagac aacctttctc tgcatgaaaa
    23341 cggtatgcgc attaccgcgc tggcgggcga taaagtcgtt tacagccaga cttactactc
    23401 tattggcggt ggctttatcg ttgatgaaga gcattttggc cagcagaata gcgcaccggt
    23461 tgaagttcct tatccgtaca gttcagcagc cgatctgcaa aaacattgtc aggaaaccgg
    23521 gctgtcactc tctggcctga tgatgaaaaa tgagctggcg ctgcacagca aagaagagct
    23581 ggaacagcac ctggcgaacg tatgggaagt catgcgtggc ggtattgagc gcggtatttc
    23641 caccgaaggc gtgttgcctg gcaaactgcg cgttccacgc cgtgctgcgg cactacgccg
    23701 gatgctggtc agccaggata aaaccaccac tgacccgatg gcggttgttg actggatcaa
    23761 catgtttgca ctggcagtga acgaagagaa cgctgctggc ggacgcgtgg tgactgcgcc
    23821 gactaacggt gcgtgcggga ttatcccggc agtgctggcg tactacgaca agttcatccg
    23881 cgaagtgaac gctaactcac tggctcgtta cctgctggta gccagtgcca ttggttctct
    23941 ttataagatg aacgcgtcaa tttctggtgc tgaagtcggc tgccagggtg aagttggtgt
    24001 ggcgtgctca atggcggcgg ctggtctggc agaactgtta ggtgcaagcc cggcgcaggt
    24061 gtgcatcgcg gcggaaatcg ccatggagca caacctcggt ctgacgtgtg acccggtcgc
    24121 cggacaggtc caggtgccat gcatcgagcg taacgccatt gcggcagtaa aagcggtgaa
    24181 cgccgcacgt atggcgctac gccgtaccag cgagccgcgc gtctgcctcg ataaagttat
    24241 cgaaaccatg tacgaaacag gtaaagatat gaacgccaag taccgcgaaa cctctcgcgg
    24301 cggcctggca atgaagatcg ttgcctgcga ttaatcgctc tccagaggcc tcgttttgcg
    24361 aggcctcttc ccgatttctc atccagccgt agcctgttcc ggcatcgaat gttaccctta
    24421 tcgcctgatc tttaaggggg ttatcgtggc tgttcatttg cttattgtcg atgcactgaa
    24481 tcttattcgt cgcattcatg ccgttcaggg atcgccctgt gtcgaaacct gccagcatgc
    24541 gctcgatcag ctcattatgc acagccagcc aacccacgcg gtcgccgttt ttgatgatga
    24601 aaaccgcagt agcggctggc gtcatcagcg tttaccagat tacaaagcgg gtcgaccgcc
    24661 aatgccggaa gagttgcacg acgagatgcc tgcattacgc gccgcctttg agcaacgcgg
    24721 cgtcccgtgc tggtcaacca gcggcaacga agccgatgac ttagccgcca cgctggcggt
    24781 caaagtgaca caggccgggc atcaggcaac gattgtttcg acagataaag gctactgtca
    24841 gttactttca ccgacattac gtattcgtga ttacttccag aaacgttggc tggatgcgcc
    24901 atttatcgat aaagaatttg gcgttcaacc gcagcagttg cccgattact ggggacttgc
    24961 ggggatcagc agttcaaagg taccgggtgt tgcgggaatc ggaccaaaaa gcgccacgca
    25021 gctgctggtc gagtttcaga gtctggaagg gatatatgag aatctggatg cggttgccga
    25081 aaagtggcgc aaaaaattag aaacccataa agagatggcg tttctgtgcc gcgatattgc
    25141 ccgcttacaa accgatttgc atatcgacgg caatttacag caattgcggt tggtacggta
    25201 acggcgagcc ggatgcaaac gattatcaac acattgaatt taatgcactt atataccgga
    25261 taagacgcgg ctagcgtcgt atccggcatt atcacatcag cgcatttacc aggcggtatg
    25321 gtaaagctct acaatatcct caagcgttgc ttcacgcggg ttgccaccgg tacaaacatc
    25381 atccagtgcc gcctgcgcca gtgccggaat gtcttccttg cgtacaccaa catcacgcaa
    25441 atgtggcgga ataccgacat cacggttgag agcaaacacc gcttcaacag cggcattacg
    25501 cgcctcttcc aggctcatac cttccacttt cacgcccata acgcgcgcga tatcgcggta
    25561 cttctcaccg gtaaagtcag cgttatagcg catgacatgc ggtaacagga tggcgttcgc
    25621 aacaccgtgt ggagtgttgt aaaacgcgcc cagtggatgc gccataccat gcaccaaccc
    25681 taacccaaca ttcgagaagc ccatacccgc aacatactgc ccgagcgcca tttcttctcc
    25741 ggcatcctta tcaccagcaa ccgatcctcg cagcgcccca gcaatgattt caatcgcttt
    25801 aatgtgcagt gcatcggtta gcgcccacgc gccacgggta atatacccct caatagcatg
    25861 agtgagcgca tcgacacccg tcgcagcttt cagcgctgga ggcataccat ccatcatgtc
    25921 agcgtcaata aacgccacct gcgggatatc atgcggatca acgcaaacaa acttgcgccg
    25981 tttctcttcg tcagtgatca cgtagttaat ggtcacttct gccgcagtac ctgctgtggt
    26041 aggaattgcc agaatcggta cactgggttt attggtcggg gaaagccctt ccaggctacg
    26101 cacatcggca aactccgggt tgttgctgat aatgccaatc gctttacaag tatcctgtgg
    26161 agaaccacca ccaatagcga tcaggtaatc cgcgccgcta ttctggaata caccgagccc
    26221 ttctttgacg acagtaattg ttgggttggg cactacgccg tcgtaaatcg cccatgccag
    26281 ccctgcagca tccatcttat cggtcacttt cgccaccacg ccgcattgca ccagcgtttt
    26341 atcggtgacg atcagcgcct tctgataacc acggcgtttc acctcatcgg ttaaagcccc
    26401 aacagcaccc cgaccaaacc atgccgtttc gttcagaatc attctgttag ccatcatcct
    26461 tctccttgtt gctttacgaa attactcttc aatccgtaac ccataggttt tgaatttctc
    26521 cagcactacg gcaatctctt catcgctcag cactggcacc gggtccgtaa tcgccagggt
    26581 cgtcacgtaa agttgcgcca gcacttcaac ttcatgcgcc agccataacg ctttttccag
    26641 attcacctca caagcgataa gcccatgatg ttgtaacaaa gttgccttac gatttttgag
    26701 agccagcgca acatgttcag aaagttcgcg tgttccaaag gtcgcataag gcgcgcaagg
    26761 aatagaatta ccacctgccg ccgcaatcat gtagtgaata gcggggatcg gtcggttaag
    26821 aatggaaact gctgtgcaat gaacggcatg attgtgaaca accgcgttgg catccggtct
    26881 gctttgatag gccgccatat ggaaacgcca ttcgcttgag gggagctttc cttcctcatg
    26941 tttaccgttg ccatcaataa agacaatatg cgactccgtc agtttttcat atggaatgcc
    27001 tgtaggcgta atcagcatcc catcctgata acgtacactg acgttccccg ctgtcccctg
    27061 gttcagtccc aggcgggtca tttccaggca agtgtcaata atctgacgag caagtttatt
    27121 tcgttccatt agctacctct ctctgattca aaacagggca ataatgttgt tcctttcaca
    27181 ctattgaatt agccgtttaa ttacccacca tcttcttcct gattaacaag aaagaaattc
    27241 acaagcttat attttgtgac ctagctcaac taatcacagt aaataactgc aagttctctt
    27301 tttataaccc cattaaaaat gaccgctctt aaaaatattt atcaaaacgg tcatttttct
    27361 attcacccaa gccagaaatg cccgttttcg gcataaacaa ttaatacagt catctggttt
    27421 gtgtttttta tgatttattt tctgaaacgg gcatgaaatt tcgattatta aagtgatggt
    27481 agtcacataa agtcaccttc cagctaataa gtgtgaccgc cgtcatatta cagagcgttt
    27541 tttatttgaa aatgaatcca tgagttcatt tcagacaggc aaatattcac tgatatgaag
    27601 cccgaactcg ctggttttgc acttttgaaa acataaccga ttacgtgctt aagcttctga
    27661 acctaagagg atgctatggg aaacacatca atacaaacgc agagttaccg tgcggtagat
    27721 aaagatgcag ggcaaagcag aagttacatt attccattcg cgctgctgtg ctcactgttt
    27781 tttctttggg cggtagccaa taaccttaac gacattttat tacctcaatt ccagcaggct
    27841 tttacgctga caaatttcca ggctggcctg atccaatcgg ccttttactt tggttatttc
    27901 attatcccaa ttcctgctgg gatattgatg aaaaaactca gttataaagc aggaattatt
    27961 actgggttat ttttatatgc cctgggcgct gcattattct ggcccgctgc agaaataatg
    28021 aactacacct tatttttagt tggcctattt attattgcag ccggattagg ttgtctggaa
    28081 actgccgcaa acccttttgt tacggtatta gggccggaaa gcagtggtca cttccgctta
    28141 aatcttgcgc aaacatttaa ctcgtttggc gcaattatcg cggttgtctt tgggcaaagt
    28201 cttattttgt ctaacgtgcc acatcaatcg caagacgttc tcgataaaat gtctccagag
    28261 caattgagtg cgtataaaca cagcctggta ttatcggtac agacacctta tatgatcatc
    28321 gtggctatcg tgttactggt cgccctgctg atcatgctga cgaaattccc ggcattgcag
    28381 agtgataatc acagtgacgc caaacaagga tcgttctccg catcgctttc tcgcctagcg
    28441 cgtattcgcc actggcgctg ggcagtatta gcgcaattct gctacgttgg cgcacaaacg
    28501 gcctgctgga gctatttgat tcgctacgct gtagaagaaa ttctaggtat gactgcaggc
    28561 tttgccgcta actatttaac cggaaccatg gtgtgcttct ttattggtcg tttcaccggt
    28621 acctggctca tcagtcgctt cgcaccacac aaagtcctgg ccgcctacgc attaatcgct
    28681 atggcactgt gcctgatctc agccttcgct ggcggtcatg tgggcttaat agccctgact
    28741 ttatgcagcg cctttatgtc gattcagtac ccaacaatct tctcgctggg cattaagaat
    28801 ctcggccagg acaccaaata cggttcgtcc ttcatcgtta tgaccattat tggcggcggt
    28861 attgtcactc cggtcatggg ttttgtcagt gacgcggcgg gcaacatccc cactgctgaa
    28921 ctgatcccca cactctgctt cgcggtcatc tttatctttg cccgtttccg ctctcaaacg
    28981 gcaactaact gaacatattt tccgaataaa gtgaggaatc tgtaatgaaa aaaatcagct
    29041 taccgaaaat tggtatccgc ccggttattg acggtcgtcg catgggtgtt cgtgagtcgc
    29101 ttgaagaaca aacaatgaat atggcgaaag ctacggccgc actgctgacc gagaaactgc
    29161 gccatgcctg cggagctgcc gtcgagtgtg tcatttccga tacctgtatc gcgggtatgg
    29221 ctgaagccgc tgcttgcgaa gaaaaattca gcagccagaa tgtaggcctc accattacgg
    29281 taacgccttg ctggtgctat ggcagtgaaa ccatcgacat ggatccaacc cgcccgaaag
    29341 ccatttgggg ctttaacggc actgaacgtc ccggcgctgt ttacctggca gcggctctgg
    29401 cagctcacag ccagaaaggc atcccggcat tctccattta cggtcatgac gttcaggatg
    29461 ccgatgacac atcgattcct gccgatgttg aagaaaaact gctgcgcttt gcccgcgccg
    29521 gtttggccgt cgccagcatg aaaggtaaaa gctatctgtc gctgggcggc gtttcgatgg
    29581 gtatcgccgg ttctattgtt gatcacaact tctttgaatc ctggctggga atgaaagtcc
    29641 aggcggtgga tatgaccgaa ctgcgtcgcc gtatcgatca gaagatttac gacgaagccg
    29701 aactggaaat ggcactgacc tgggctgata aaaacttccg ctatggcgaa gatgaaaata
    29761 acaaacagta tcaacgcaat gccgagcaaa gccgcgcagt tctgcgcgaa agtttactga
    29821 tggcgatgtg tatccgcgac atgatgcaag gcaacagcaa actggccgat attggtcgcg
    29881 tggaagaatc acttggctac aacgccatcg ctgcgggctt ccaggggcaa cgtcactgga
    29941 ccgatcaata tcccaatggt gacaccgccg aagcgatcct caacagttca tttgactgga
    30001 atggcgtgcg cgaacccttt gtcgtggcga ccgaaaacga cagtcttaac ggcgtggcaa
    30061 tgctaatggg tcaccagctc accggcaccg ctcaggtatt tgccgatgtg cgtacctact
    30121 ggtcaccaga agcaattgag cgtgtaacgg ggcataaact cgatggactg gcagaacacg
    30181 gcatcatcca tttgatcaac tcaggttctg ctgcgctgga cggttcctgt aaacaacgcg
    30241 acagcgaagg taacccgacg atgaagccac actgggaaat ctctcagcaa gaggctgacg
    30301 cttgcctcgc cgctaccgaa tggtgcccgg cgatccacga atacttccgt ggcggcggtt
    30361 actcttcccg cttccttacc gaaggcggcg tcccgttcac catgactcgt gtcaacatca
    30421 tcaaaggcct gggatcggta ctgcaaatcg cggaaggctg gagcgtggaa ttgccgaagg
    30481 atgtgcatga catcctcaac aaacgcacca actcaacctg gccaaccacc tggtttgcac
    30541 cgcgcctcac cggtaaaggg ccgtttacgg atgtgtactc ggtaatggcg aactggggcg
    30601 ctaaccatgg ggttctgacc atcggccacg ttggcgcaga ctttatcact ctcgcctcca
    30661 tgctgcgtat cccggtatgt atgcacaacg ttgaagagac caaagtgtat cgtccttctg
    30721 cctgggctgc gcacggcatg gatattgaag gccaggatta ccgcgcttgc cagaactacg
    30781 gtccgttgta caagcgttaa tacattactc cctgatgtga tgcccggtcg ctccggctac
    30841 cgggcctgaa caagcaagag tggttagccg gataagcaat gttatccggc tatattgcag
    30901 gagcgattat gaaacaagaa gttatcctgg tactcgactg tggcgcgacc aatgtcaggg
    30961 ccatcgcggt taatcggcag ggaaaaattg ttgcccgcgc ctcaacgcct aatgccagcg
    31021 atatcgcgat ggaaaacaac acctggcacc agtggtcttt agacgccatt ttgcaacgct
    31081 ttgctgattg ctgtcggcaa atcaatagtg aactgactga atgccacatc cgcggtatcg
    31141 ccgtcaccac ctttggtgtg gatggcgctc tggtagataa gcaaggcaat ctgctctatc
    31201 cgatcattag ctggaaatgt ccgcgaacag cagcggttat ggacaatatt gaacagttga
    31261 tctccgcaca gcggttgcag gctatttctg gcgtcggagc ctttagtttc aatacgttat
    31321 ataagttggt gtggttgaaa gaaaatcatc cacaactgct ggaacgcgcg cacgcctggc
    31381 tctttatttc gtcgctgatt aaccaccgtt taaccggcga attcactact gatatcacga
    31441 tggccggaac cagccagatg ctggatatcc agcaacgcga tttcagtccg caaattttac
    31501 aagccaccgg tattccacgc cgactcttcc ctcgtctggt ggaagcgggt gaacagattg
    31561 gtacgctaca gaacagcgcc gcagcaatgc tcggcttacc cgttggcata ccggtgattt
    31621 ccgcaggtca cgatacccag ttcgcccttt ttggcgctgg tgccgaccaa aatgaacccg
    31681 tgctctcttc cggtacatgg gaaattttaa tggtccgtag cgcccaggtt gatacttcgc
    31741 tgttaagtca gtacgccgat tctacctgcg aactggatag ccaggcaggg ttgtataacc
    31801 caggtatgca atggctggca tccggcgtgc aggaatgggt gagaaaactg ttctggacgg
    31861 ctgaaacacc ctggcaaatg ttgattgaag aagctcgtct gatcgcacct ggcgcggatg
    31921 gtgtgaaaat gcagtgtgat ttattgtcgt gtcagaacgc tggctggcaa ggagtgacgc
    31981 ttaataccac gcgggggcat ttctatcgcg cggcgctgga agggttaact gcgcaattac
    32041 agcgcaatct acagatgctg gaaaaaatcg ggcactttaa agcctctgaa ttattgttag
    32101 tcggtggagg aagtcgcaac acattgtgga atcagattaa agccaatatg cttgatattc
    32161 cggtaaaagt tctcgacgat gccgaaacga ccgtcgcagg agctgcgctg ttcggttggt
    32221 atggcgtagg ggaatttaac agcccggaag aagcccgtgc acagattcat tatcagtacc
    32281 gttatttcta cccgcaaact gaacctgaat ttatagagga agtgtgaaat gctgaaaaca
    32341 atttcgccgt taatttctcc cgaactattg aaagtgctgg cagagatggg acatggagat
    32401 gaaattattt tttccgatgc tcactttccc gcccattcga tgggaccgca ggtgatccgc
    32461 gctgatggcc tgttggtgag cgacttgctc caagcgatta tcccattatt tgagctggac
    32521 agttatgccc cgccgctggt gatgatggca gcggtagaag gtgacactct cgatcctgaa
    32581 gtagaacgac gttaccgtaa tgcgctttca ctacaagccc cgtgtcctga catcatccgc
    32641 atcaatcgtt ttgcgtttta tgaacgggcg caaaaagcct ttgcgatcgt tatcacaggc
    32701 gaacgagcga agtacgggaa tattctttta aaaaaagggg taacaccgta atctcatatc
    32761 ggtacgcccg catgacgcgg gcggttatcg aatgatgggg tgaaaaatat gaaagcggca
    32821 cgccagcaag cgatagtcga cctgctgctg aaccatacca gcctgaccac ggaagctctc
    32881 tctgaacagc taaaggtcag taaagaaacc attcgtcgcg atctcaatga attacagacg
    32941 cagggtaaaa ttctgcgcaa tcatggacgc gctaaatata tccaccgtca aaatcaagac
    33001 agtggcgatc cctttcacat caggctgaaa agccattatg cgcataaagc agatatcgcg
    33061 cgcgaggcgc tcgcgtggat tgaagaaggg atggtgatag ccttagacgc cagttcaact
    33121 tgctggtatc tggcacgcca gttgcctgac atcaacattc aggtcttcac caatagccat
    33181 ccgatttgcc atgaactcgg taaacgcgaa cgcattcaac tgatcagttc cggcggcaca
    33241 cttgagcgca aatatggctg ttacgtcaat ccctcgctga tttcccaact taaatcgctg
    33301 gaaatcgatc tgtttatttt ttcttgtgaa gggatcgata gcagcggcgc actgtgggac
    33361 tccaatgcga tcaacgctga ttacaaatcg atgctattaa aacgtgccgc gcaatcgttg
    33421 ttattgattg ataaaagtaa atttaatcgt tcaggggaag cccgcatcgg gcatctggat
    33481 gaggtaacgc acattatttc tgatgagcgc caggttgcaa cttctttggt aacagcctga
    33541 cggaaagggt agcaggccga agacgacgcc ccggccttgc ctgttatcgc tcgtcgcgac
    33601 gaccacccac cgcagcccag atgcggcgga cgtgcaccgt cacttcttcg cgatcgtgat
    33661 acaactgccg cgcctgaatc tgagcattta tgccatgttc atcaagctgt gcctgaatat
    33721 acgccagatt gtgtgatact tcttcgtagc gttttttcat cggcagtttg agattgaaaa
    33781 tggtttcacg gcaccagcca ttaaccagcc actgcgccat caatgccgca actttagccg
    33841 gtttttcaac catatcgcat accatccagg agatattgct gcgtgtcgga cggaatttga
    33901 aaccgtcttc ccgcagccac gtcacctgcc cggtatccat cagactttgc gccatcgggc
    33961 cgttgtcgac ggaataaacc cacatgttgc gcttcaccag ttggtaggtc cagccgccag
    34021 ggcaagcgcc taaatccacc gcccacatcc cgttcgccag gcgttcatcc cactcatccg
    34081 caggaataaa cacatgaaat gcctcttcca gtttgagcgt ggaacgactc ggcgcctctg
    34141 ccggaaattt caggcgcgga atgcccatat agaacggcga attattgttg ctgtatgagt
    34201 aaccggtata gcagcagcct ggtgcaataa agaatacatg cacaaccgga cgcttcggcg
    34261 tttcatagtt cgccagcacc ccggcatcgc gcaaggcagc gcgtagcggc acggtgaatt
    34321 tacggcagaa tttcagcaac tctttgcttt cgttggtatc ggcaacttca acacgcagtt
    34381 caccgccctt ctctactacg ccctgcaaca tgtcgacaat gggggtaata cgatcttctg
    34441 gcggcaaatg ctgcaagagt tcccccacca caaaccactg gcgggcaaaa attaatgaac
    34501 tgaacggcag ctcacggatt aacttatcgc catcatcagg ttgataacat tcataaatga
    34561 cataacccgc attctctttc acgcgggcaa aaccgaaaat ttcccgctgg ccggctttat
    34621 cggtaatttc tgcggcgcac tctttttcaa agcccggacg gcacagcaat acaaccttat
    34681 tcatgactta cgcccttgca ctttaaacgg atagcaccaa ctaacattaa cgcccagccc
    34741 gcgaggaagc tcacgccgcc aaccggtgtg acaaatgccc acaaacgcag atgggacagc
    34801 gccaggcaat aaaggctgcc gctgaacaac accgtgccta acgcgaggaa aacgctactc
    34861 cagtaaaacc agatactgat gcgacgctgc attgccaccg ccagacctaa gatcgccagc
    34921 gtatgaaacg cctggtattc gaggccggtc tggatccagc ccatctcaac ggcccccatg
    34981 gttttactta acacatgcgc gccaaaagcg cccagagcca caaaaatgaa gccgctaatg
    35041 gcggcgaaaa tcagcataaa acggctggtc atggtcgtac cctacgtaaa ttattgttca
    35101 taacgaaagc ggaatttttc ttgttcagca gcggctttcg ccaggatcca ttggcgaaag
    35161 gcggctattt tacccagttc tgcctggctg tcatgacaaa ccagataaaa agcgttttta
    35221 ctgaccagaa catcattaaa cgggcaaaca agacgtccgg cctcgatttc agattgcgcc
    35281 atcacgttat ttgccagcgc cactccctgc ccgtgaatag ccgcttgcag caccatggcg
    35341 ctatgactaa aaattggccc ttgctgaacg ttgatatgat ttaaccccaa ctgtcgggta
    35401 tatgtctgcc agtcacggcg tgacgcatca tgtaataacg tatgtttagc cagatcttcc
    35461 ggggtcttca agggtttttc gcctgtcagc agtagcggcg aacacaccgg caataaatat
    35521 tcggcgtaca gtttttccac ccgtagcccc ggccagttgc cccgaccata aaatatcgcc
    35581 acatcaacat catccgccag cttatcttcc tgacgatcaa ccgcctggat tcgaacgtca
    35641 attcccggat aagctgaatt aaagctggaa agtcgcggaa ccaaccaatg aatggcgaaa
    35701 ctggggagta aactgaccgt caacgccccc ttggcgctac gggcctggag tttacgcgtc
    35761 gcttcggtta attgcgaaaa tatctctttg atatcgagga aatagctttg cccttcctcg
    35821 gtcagcagga gtgaacgatt acggcggcgg aacagtttta gccccaaaaa atcctcaaga
    35881 gacttgattt gatgacttac tgcggcctgg gtcacgaaaa gctcttctgc tgcgcgagtg
    35941 aaacttaaat ggcgtgctgc ggcatcaaaa actcgtaagg catttagcgg tggtaatcgt
    36001 ttagacatgg cttttaaact ttgatgttaa attaatttaa caattagatc acactgtgta
    36061 acctattagt ttttttaatc tgagccatta taaattgtcc gttgagcttc taccagcaaa
    36121 tacctatagt ggcggcactt cctgagccgg aacgaaaagt tttatcggaa tgcgtgttct
    36181 ggtgaacttt tggcttacgg ttgtgatgtt gtgttgttgt gtttgcaatt ggtctgcgat
    36241 tcagaccatg gtagcaaagc tacctttttt cacttcctgt acatttaccc tgtctgtcca
    36301 tagtgattaa tgtagcaccg cctaattgcg gtgctttttt ttaccttgcg atcgcgaatt
    36361 actgatccag ttcgaccatc tctttcacgt cagtacggtt gatctgctgt ttgttgccat
    36421 tagcgtcttt atacgaaatc ataccggtat cgttatcagt ctgtggtttg ccgtcagaga
    36481 cgatggtacg tccgtcattg gtgtgcatca catagttcga accggaacag gcgctcaggg
    36541 caaaagtcag catacaggca gaaataattg cggcagtctt tttcatcatc ttctccttaa
    36601 agcgattaat aaatcttaat agtattgatt aacaggctaa aattaacgcc taacactatt
    36661 cagcatatgt tacttacgcg gcttcgccag gatatccaga taattctgat ggttagcact
    36721 ctccttgtat caaagtgaat tttgcgtcac gatcggtgca tcaagccgag gagtaccatg
    36781 aacgttttta atcccgcgca gtttcgcgcc cagtttcccg cactacagga tgcgggcgtc
    36841 tatctcgata gcgccgcgac cgcgcttaaa cctgaagccg tggttgaagc cactcgacag
    36901 ttttacagcc tgagcgccgg aaacgtccat cgcagccagt ttgccgaagc ccaacgcctg
    36961 accgcgcgtt atgaagctgc acgagagaaa gtggcgcaat tactgaatgc accggatgat
    37021 aaaactatcg tctggacgcg cggcaccact gaatccatca acatggtggc acaatgctat
    37081 gcgcgtccgc gtctgcaacc gggcgatgag attattgtca gcgtggcaga acaccacgct
    37141 aacctcgtcc cctggctgat ggtcgcccaa caaactggag ccaaagtggt gaaattgccg
    37201 cttaatgcgc agcgactgcc ggatgtcgat ttgttgccag aactgattac tccccgtagt
    37261 cggattctgg cgttgggtca gatgtcgaac gttactggcg gttgcccgga tctggcgcga
    37321 gcgattacct ttgctcattc agccgggatg gtggtgatgg ttgatggtgc tcagggggcg
    37381 gtgcatttcc ccgcagatgt tcagcaacta gatatcgatt tctatgcttt ctctggtcac
    37441 aaactgtatg gcccgacggg tatcggcgtg ctgtatggca aatcagaact gctggaagcg
    37501 atgtcgccct ggctgggtgg cggcaaaatg attcacgaag tgagttttga cggcttcacg
    37561 actcaatctg cgccgtggaa actggaagca ggaacgccaa atgtggctgg tgtcatagga
    37621 ttaagcgcgg cgctggaatg gctggcagat tacgatatca accaggcaga aagttggagt
    37681 cgcagcctgg cgacgctggc ggaagatgcg ctggcaaaac gtcctggctt ccgctcattc
    37741 cgctgccagg attccagcct gctggccttt gattttgctg gcgttcatca cagcgatatg
    37801 gtgacgctgc tggcggagta cggtattgcc ctgcgggccg ggcaacattg cgctcagccg
    37861 ctactggcag aattaggcgt aaccggcaca ctgcgcgcct cttttgcgcc atataataca
    37921 aagagtgatg tggatgcgct ggtgaatgcc gttgaccgcg cgctggaatt attggtggat
    37981 taatgacgaa cccgcaattc gccggacatc cgttcggcac aaccgtaacc gcagaaacgt
    38041 tacgcaatac cttcgcaccg ttgtcgcaat gggaagataa atatcgccag ttgatcatgc
    38101 tggggaaaca gcttccggct ttgccagacg agttaaaagc gcaggctaaa gagattgccg
    38161 gatgcgaaaa ccgcgtctgg ctgggatata ccgtgactga aaacggtaaa atgcacttct
    38221 ttggcgatag cgaagggcgc attgtgcgcg gcctgctggc ggtgctgttg actgccgttg
    38281 aggggaaaac cgccgccgaa ttgcaggcac agtcgccgct ggcattgttt gatgagctgg
    38341 gattgcgtgc gcaacttagc gcctcacgca gccaggggtt aaatgcgtta agcgaggcga
    38401 ttatcgctgc gacgaagcag gtttaagcat agaaccgggt cggataagac gttcgcgtct
    38461 catccgacct gattgtttaa ccctgacgcg ccgctttcgc catcatcttc ttcagcgcat
    38521 gagaaaccgc aacaaaccca aaggtggcgg tcaccatcgt tgccgcgcca aatccagatg
    38581 cacaatccat ccgcttcggc ccttctgccg tggctttcat cgcacacaca gtaccgtctg
    38641 actgcgggta caccagcgct tcagtagaaa acacgcaatc gacgccgagc ttacctttac
    38701 tgtttttcac tacgccaaaa tcgcttttca ggcgctcgcg caacttcgcc gccagcggat
    38761 cctgaatcgt tttcgccaga tcggtaacct gaatctgcgt cgggtcaatc tgcccacccg
    38821 cgccaccggt cgtgaccagc gggattttat tgcgccgaca ataagcaatc agcgccgctt
    38881 tgggccgtac actatcaatg gcatcaatca cgtacgaata acccacgctc atatactgcg
    38941 ctacgttatc cggcgtcacg aaatcatcca ccaccgttac acggcactcc gggttaatct
    39001 ggcgaatacg ctccgccatg acttccgctt ttgccagccc gacgttatcg cgcagagcgt
    39061 gaatttgccg attggtattg gtgacgcaca catcatccat atcgataagc gtgattgcgc
    39121 caatccccgt gcgcgccagc gcttccgccg cccaggaacc gacaccaccg ataccaacca
    39181 cacaaatatg cgcgtcagca aacagttgca acgctttttc accatacaga cgcgctgtgc
    39241 caccaaaacg ctgacgccat gcatcactaa ttaccacaga catagcacct cagaattaaa
    39301 aagggtgagg ttatccctca ccctgtcgta tccgtaaaaa cggtatacag aataacacat
    39361 cagccgctaa agacgttacc tgcgcccggg gcggttttca gcacccagac acgtccatag
    39421 tggttgtacc aacctgcgcg gtgtccggct tccggcccga tcccttgata gatatcgaag
    39481 tgttggcctt tgattgcacc accgacatcc agcgccacca tcagacgcag ttcgtactga
    39541 ccattaaatt tgccgttatt atccagcaac ggcacttctg ccagcaaggt agtacctggc
    39601 ggaataatgg aacgatcaga ggcaactgac gcgcgaccaa ccagcggcac cgcacttgcc
    39661 cctttcaccg gtgcaaaaga ttgcggttta aagaagacga aagacgggtt ctgttccagc
    39721 agctcgcgaa cctcggcttc actgtgtgtt tcgccccagt gacgaatcgc ctgcatcgac
    39781 atatcttctt ttttcacttc gccacggtcg atcagcacct taccaatgct gcgataggca
    39841 tgaccgtttt tccctgcata gctgaaaaag ttaagcggac tgccatcacc aaagtcgata
    39901 tacccactac cctgcacatc cataatgaag ttatccatca gggagttact gtaagcgaga
    39961 atatatttat cactcaatgc ccccgcgtag atctccgcac gagacggcag acgaccacgt
    40021 tttggcggca tacggtaaat aggatactgg aactcgccct ggcgggtatg gcgcgcctga
    40081 attaccggcg tgtaatagcc tgtaaactgc acgttaccat agttgtcggc accttccatc
    40141 tgccaggcat caatgccgaa ctggcgcata ttgcgggtat caccgcctgc gcgcagccac
    40201 tcttgcaccg cgttataaac attactctgg ttgccataca gacgcggtga cgaattacgg
    40261 atatggttaa tttgctcggc aaaatcaccg gcgttaatcg gcgcgccaac ggcatctggc
    40321 tggttcacca gagagaaagg ctgggtaaat ttcccgtctt tatattgctg tccgcgatcg
    40381 gttggtttgg aagagcaggc ggcaagcatt gccacaaccg tgcccataag aaggtacttt
    40441 acccaacgtc ctttcattgt tctcttcttc aggttaaaaa ataaggcgca acgaagataa
    40501 caaaccgccg cgtttaatga aatgagcagc ctctccctga cgcaaatttt gcacaaaaaa
    40561 taggctttag tgatttgttt ttgttcaaaa tcatgccaga tccgtgatcg gggtaaaaaa
    40621 aaggttgcat gaaaacgcga gcggagtata gtgcgcatcc acggaggcgg ggtggagcag
    40681 cctggtagct cgtcgggctc ataacccgaa ggtcgtcggt tcaaatccgg cccccgcaac
    40741 caattattga acaccctaac gggtgttttt ttgtttctac tctcccataa aaaaagcgcc
    40801 attcagcgcc tttttatcat ccccttctcg ccagcgttgc cccatcggca aaatacgctt
    40861 taatccccgc aagaatagac tccgcaactt cctgctggaa agtcgccgtt ttcagtttac
    40921 gctcttcctc aacgttactg ataaacgccg tttcgaccag aatggaggga atatctggtg
    40981 cctttagtac ggcaaacccg gcctgttcaa cttgattttt atgcagcttg ttgattttac
    41041 cgagcttatt cagcactgct ttaccaaact taaggctgtc agcaatggtc agcgactgca
    41101 ccatatcgaa catggtgtgg tcgacatagc ggtcaccgct tttgctcacg ccaccaatca
    41161 agtccgaggc gttctgggtt tgtgccagat attttgccgc agtactggtt gcacctttgg
    41221 ttgagagcgc aaacaccgag gaaccgctcg gctggcgact ggtaaaggcg tcggcatgga
    41281 tagagacaaa caggtcagca cgctgtttct gggcttttgc tacgcgcact tgcaacggaa
    41341 tgaagatgtc ttcattgcgc gtcatgtaca ccttcatatt gccctctttc tcgatcagag
    41401 agcgcaaacg gcgagctatt tgcaatacca catctttttc gcgcgttttg tatttcccca
    41461 ccgcaccgga gtcttcgcca ccgtggccag ggtcaagcat aatgacaatc ggacgatcgc
    41521 gccctgcttt acctggttgt ggaccacttt gcgctggcgg cacctgcttt tcgaggtcgc
    41581 ctttgttgta atcctccagc agcgccagca gcgggtcctg catatcctgt gcattggccg
    41641 gatagaggtc catcaccaga cgctctttaa acccggcgac cggcgcaagg gcaaacagct
    41701 gcggttttac gttttgcttt aattcaaaaa ccatccgtac ggtttgcggg tcaaattgcc
    41761 cgacgcgcgc cgacttgatg aacgggtcgt ctgcacgaat ttgtgccgcc atccccttga
    41821 gtaccgagtt caggtttaca tcttcgatat ccaccaccac gcgttcaggg ttactcaacg
    41881 cgaactgctt atatttcagc tgacgatttg attctaccgt cacgcgggtg tagctggacg
    41941 caggccagac gcgcaccgcc acgacctggc taaccgcagc caggctgacc tgacttacgc
    42001 tcaatagcca catagcgccc gcgccttgca gtaaacgacg acggctgatt gcagtgttgg
    42061 atcctgacat gcctctcccg agcaaaagaa atctaagctg tgtaacaagt aaacgactaa
    42121 tttgaccgga aactttagcg aaagacgcat aatctgtcat ctaataaacg gtaaacattc
    42181 tttttatatt cacggcatta ctgataaaaa agtcgctctc gcataaaatt tacacttgca
    42241 ccctgcgaaa aaacagaata aaaatacaat aatttcgaat aatcatgcaa aggggtgtgc
    42301 cgtggtaaag gaacgtaaaa ccgagctggt cgagggattc cgccattcgg ttccctatat
    42361 caatacccac cggggaaaaa cgtttgtcat catgctcggc ggtgaagcca ttgagcatga
    42421 gaatttctcc agtatcgtta atgatatcgg gttgttgcac agcctcggca tccgtctggt
    42481 ggtggtctat ggcgcacgtc cgcagatcga cgcaaatctg gctgcgcatc accacgaacc
    42541 gctgtatcac aagaatatac gtgtgaccga cgccaaaaca ctggaactgg tgaagcaggc
    42601 tgcgggaaca ttgcaactgg atattactgc tcgcctgtcg atgagtctca ataacacgcc
    42661 gctgcagggc gcgcatatca acgtcgtcag tggcaatttt attattgccc agccgctggg
    42721 cgtcgatgac ggcgtggatt actgccatag cgggcgtatc cggcggattg atgaagacgc
    42781 gatccgtcgt caactggaca gcggtgcaat agtgctaatg gggccggtcg ctgtttcagt
    42841 cactggcgag agctttaatc tgacctcgga agagattgcc actcaactgg ccatcaaact
    42901 gaaagctgag aagatgattg gtttttgctc ttcacagggc gtcactaatg acgacggtga
    42961 tattgtctca gaacttttcc ctaacgaagc gcaagcgcgg gtagaagccc aggaagagaa
    43021 aggcgattac aactccggta cggtgcgctt tttgcgtggc gcagtgaaag cctgccgcag
    43081 cggcgtgcgt cgctgtcatt taatcagtta tcaggaagat ggcgcgctgt tgcaagagtt
    43141 gttctcacgc gacggtatcg gtacgcagat tgtgatggaa agcgccgagc agattcgtcg
    43201 cgcaacaatc aacgatattg gcggtattct ggagttgatt cgcccgctgg agcagcaagg
    43261 tattctggtg cgccgttctc gcgagcagct ggagatggaa atcgacaaat tcaccattat
    43321 tcagctcgat aacacgacta ttgcctgcgc cgcgctctat ccgttcccgg aagagaagat
    43381 tggggaaatg gcctgtgtgg cagttcaccc ggattaccgc agttcatcac ggggcgaggt
    43441 tctgctggaa cgcattgccg ctcaggcgaa gcagagcggc ttaagcaaat tgtttgtgct
    43501 gaccacgcgc agtattcact ggttccagga acgtggattt accccggtgg atattgattt
    43561 actgcccgag agcaaaaagc agttgtacaa ctaccagcgt aaatccaaag tgttgatggc
    43621 ggatttaggg taagcgatga aaatcgccgg atgcggcagg cgtaacactc gtgcgtcgca
    43681 tccggcaatt acgtttatcc ccgcgaacta aacaacgccg ccagaccact gcgccgctca
    43741 gtacgagtgg cgattgccgc acttaatata cgctcatcgg catacagcga cagacggcga
    43801 cgcgcgcggg tcaccgcggt gtataccagc tctcgcgtta ctatcggcgt gcgttggctc
    43861 ggtaaaatca acgccgcatg gtcgaactcc gatccctgcg atttatgtac cgtcatcgcc
    43921 cacgtagttt cgtgctctgg caagcgactc ggttgcacag acttaatatt gccgtccggc
    43981 atcgcaaacc agacgcgcgt cccctgcccg cgatccagcg caataccgat atcgccatta
    44041 aacaacccaa gcgcactgtc attacgggca atcatcaccg gtcgaccttc gtaccaacga
    44101 gagtgcgaat gacgatgaat tttgcgcttc tgttgcataa actgctcaat tcgctcattc
    44161 agtccagcca cgccaaatgg cccttcccgc agggcgcaca aaagttggta ctcattgaac
    44221 gcctgaatga ttaaatccgg ctcggcacgc gcttgcagca gatccagata acgtccataa
    44281 cccgcaagag cttcctcaag catcgcaata taatcttcgc cgctctgtaa aagccgtttt
    44341 tcaatatcag taaaatcctg ctgaaaaacg gttttcactg ccgttttatc accacggttg
    44401 atcgccgcag ctaactgacc aatgccagaa tcgctgccga aacgatagct tttttgcagc
    44461 aggcagagac tgtcgcgcaa agatgccgct tctgtgccgg ttcctgccgg aacgtgagtt
    44521 cccgtcaggc ggcttagctg cccggcacgc tcggcggtaa agccggcatt ggcataagcg
    44581 cagatatcgc ccagcacagc cccagcctca accgaggcca gttgatcacg atcgccgaga
    44641 aagatcactc gcgcatgatc gggcaaggcg tcgatcagtc tcgacatcat aggcagatcg
    44701 atcattgacg cttcatctac caccagcaca tcaagatgca gcgggttacc ggcatgatga
    44761 cgtaaacgct ggctacccgg ctgcgcgccc agcaatcggt gcaaagtgct ggcatcttcc
    44821 ggaatgcgtt tcttttgttc atcggtcagc ggtaactgtc gcaaagcctt gccgagagat
    44881 tcggttaagc gcgcggcagc tttacccgtt ggtgcagcca gacggatacg gcagcgttcg
    44941 ccgtcggcca tttgaattaa cgctgccagc aacttcgcca cggtggtcgt tttaccggtg
    45001 cctgggccgc cggaaatcac cgagatccgc cgcgtcagcg ccactgctgc cgccactttt
    45061 tgccagttaa tttcatcgct tactggaaaa agtttgtcca gggtttgcgc cagtagagct
    45121 tcatcaacct caatggcatg attcacctca ttgaagaagc gcgctaccgt ccgctcgtta
    45181 caccacatgc gattcaaata aagacgatcg ccacagagga tcatcggcgt gggttcatct
    45241 cccctgctga ccgcttgaga agccagcaag cattcttccc aattttgtag ctcaccgatt
    45301 tcactgacac aggtcgccaa cagcggatgc gacgcctcgt tattttccag tcgtgaaagc
    45361 ggcaaacaga cgtgtccctc tccggcatca tgacttaaca gtgccgccgc gagggtgacg
    45421 gcaggatgtt catctcccgc cacggtcagg gcaaattgca catccagcgg gcgtagctgt
    45481 ttgtgctcca cagcttccag taattgcttt tgcaatttca ttacgcctcc tccagggtca
    45541 taccggcaaa catctcatcc atcagggcaa tcaacccggc gttgggtcgg gttgtgtaaa
    45601 tcccttgttg cggatgttct ttatcaacgc cacgcaggaa cagatagata acgccgccaa
    45661 agtgacgatc atagtcgtaa tcagcaatgc gatggcgcag ataacgatgc agcgccaggg
    45721 tataaagctg atattgcaga tcatagcggt gtgcctgcat cgctgctgcc atagcctgtt
    45781 gggtgtaagc cgaactgtct tcacccagcc agttggattt atagtcgagc aggtaataac
    45841 gcccttcgtg gcggaatacc aggtcgataa agccttttaa catgccgcgt acctgcatga
    45901 actccagcgg cgggcagcca gcggatagcg ggtcaaactg gcgaattagt gcatcaagct
    45961 ggctggcgat gagcggttcg ctaatcggca ggtaaaactc catctccacc tgtttatcgc
    46021 ggtcggaaag ctgactcagg ctaacacccg tttcattgag aggtgcctgg aggacagccg
    46081 tgatccactc ggtcaacacc ggttcccact gcgattcaaa gccgccgagt tccagttttt
    46141 cctgtaccca gttcgggtca accggctggg taaaatcgag gtcttcaaac aaactgtgca
    46201 agaatgtccc cggtgacgca ccgcgcggga actgatgcgg tgttaacgtc ggttcttcaa
    46261 cgacgctgac cacgcctgcg gcatcaacat ccagccgtgg catcagatcc tgagcgatac
    46321 cgtgaccacg ctgctgcaag ccggagtagc tggtgacacg ccagttatcg ccgggcaatc
    46381 gttgtagtgt cctcgcattc agttctgcag ttaaagcatc attaacctgc cagggctggt
    46441 tatcaccagt ttgtgccgtt tgccaggcaa tatcatcatc gcataacgct tcaatacagg
    46501 tgcgaagccc tgccgcatct tgcggttccc ctttttgcag caaacgcccg agcgcacttt
    46561 ggtggacgtc ggtgtcacct tttttatcgc cacgacggcg caccagcggt gcaacgccga
    46621 gactgcaatg ccaaaccgaa cgcgtcagcg ccacgtaaag caaacgcaga tcttccgcca
    46681 gacgttcgac ctccgcgagg tcgacgcttt ctggcgcagc attaagatcc aaaactgcct
    46741 caaacgagtg gcgatcgtga taaaacgcct gatcctggac gcggaaattg gtaataaatg
    46801 gcaaccagac cagcggatat tccagccctt tcgatttatg gatcgtgaca atctgcacca
    46861 gatgtttatc actttcgagg cgcatttgtt ggctggaggc attactgtct ggctcgagga
    46921 tatgttgcga taaccagcgt accagcgcat gttcactttc cagctgcgtt ccggcttctt
    46981 gaagcagttc gctgatatgc aagatatcgg taagacgccg ctcaccgcct gccgttgcca
    47041 gcaagttttc tgcaatgtta cgcgccgaca tcagcgcccg cagcatcggc ataacgccac
    47101 gtttgcgcca gatttgccga taaccatcga actcttcgac taccgcatcc cacgcatgtt
    47161 cgtcattatt cagcgtttca atatccagcg cattcagccc catcattgac gttgccagcg
    47221 cactgcgcag ggtgttctca cgttcgggcg tcatcaccgc ctgcaacagc caaagcattt
    47281 cctgcgcttc cagagtttca aaaacactat cgcggttcga aaggtaaacg gaagggattt
    47341 ccagcaacgt taaggcatcg cgcacctggg cggcctcctg gcggctgcgc accagcacgc
    47401 tgatgtccga agcacgcacc ggacgcgcgt cgtcgccgtt catcagcaac gcttcgcccc
    47461 gctgtccggc ttgtagccag tcgcggattt gcgcagcaca tacctgcgcc atggtacttt
    47521 gatagtcgcc aacgccgcag ctttcgcctt ccatcagcca cattttcatc gcaggctgcg
    47581 tttcaccttt aaagacaaaa cgtaacgcct gatttttccc ggctgatttc actggaataa
    47641 acggtatttc gcgaaacatg aacgcgtcat cagtctggct gaaaagcttg ttcacactgt
    47701 tcaccattcc aggtgcggag cgccagttgg tgtctaaagt gtagtgggcg tgaacttcac
    47761 tacgcgcctt catataagtg aagatatccg caccccggaa tgcatatatg gcctgcttcg
    47821 ggtcgccaat cagcaacaat gcggtttctg gctgatggtg ccagatacgg cgaaaaattc
    47881 ggtactgctg ggggtcggta tcctgaaatt catcgatcat tgccaccggg aatcgcgtac
    47941 ggatcgccgc tgccaacacc tcgccgcttt cgctacgcag cgcggaatcg agccgactta
    48001 acatgtcatc aaaacccaat tcgccacggc ggcgtttttc acgcgctact gtttcgcgga
    48061 tctcagccaa tgcgcgggtg atcaccagat cacggatcga caatggttct gcaagcagtt
    48121 gatcgatcgc ctcaaacagt ggatgtcgcg gggtttcccc cccggctttc gtgcgatctt
    48181 ctaagaaacg ctgggagaat ttttccagcg actccggcaa ctgataactg tttgtctctt
    48241 cttctgccca ggcgctgatc ttgtcgatcc atttagcctg attgctacgg ttaaacttgc
    48301 gtcgatcaat accagaagat tcgatcagcg catccagttc acccactgcg tcgcgccact
    48361 gctgttttac cgtatcaata cgcgccacaa tttgcgcgtg acgggaagct agcgtttcat
    48421 catcgggcgg cggtgctttg ataaccggcg cttcgccttg cagataacga ttaatatcgc
    48481 gcagcaacgc ctgcggccct ttccaggttt caaagacgac ctgggctatt tcacgcggca
    48541 gcgggtagca gtggcgacgc cagaaatcgg cgcaggcctg gtagcgtagc agagactcat
    48601 cttcaatcag ctgctgctca aacagcatgc cggattcaaa ggcattcagg ttgagcatgc
    48661 gctggcaaaa gccgtgaata gtaaagactg ccgcttcatc catctgccgt tcggctaaca
    48721 acaaccactg cgcggcttgc gctttatcgt cgatctcttc cagcaggtgt tcgtacaatg
    48781 gattgtcggt ggtttcacgc agacaggcga tgcgcaactc gtggatattg ctacggatac
    48841 gaccgcgcaa ttctgccgtg gcagcctcgg taaaggtgac caccagcagt tcttcaacgg
    48901 tcagcgggcg gggaaaggcg gcggaaccgc ctagtccaag taacaggcgc aaataaagcg
    48961 ccgcaatcgt aaaggttttg cctgtgccgg cagaggcttc aatcaggcgc tcgccctgta
    49021 agggcaagcg caaaggatct agtgtctcgg cgacatcact cattcttttc actcatcagg
    49081 ggcattgttt gctgcaacgc gctgacgttc tcccacactt tccagccttc aggatgcaca
    49141 tattcggctt tcccgttttg gctgccggaa atctgcgaca ggatagccat gccttgcggc
    49201 tcgactaccg cctgatggaa gaaatcggcc agtttttgcg gcgtcagcag tttaatctgg
    49261 gccacgattt tatcacgcga atcgaagcgc atattgccgc gatcgaaatc tttacttaac
    49321 ttcgatgctt cttcgccgag cgtttgcggt gcctgcagca tctgggtaat taccgcctgc
    49381 tggatttgcg caaactcatc tggcttcatc gctcgcaatt ttgcctctgc ggttgggaaa
    49441 aacgccttgt aacgctccca caagaatgaa ggctgtttat cattgctttg caaaaggaag
    49501 cccatgcccc actgacgccc cacgctcatt ggaaacgcaa acacggcata gcccaattgt
    49561 tcttcggtac gcaactgatt gtagaaccac ggctgtacga tctgccccaa cagagagcta
    49621 taggctgagc tggtgtattc atcgtagcca gtcggtacaa ataccgctgc cagtgcggag
    49681 tcggtgctgt taccggcttt ttcaaagatg acggattgtt ttttatcgac cactacatct
    49741 ttgtttcgac accactctga accatcagcg cccaactgtt tttgcacatc gcgtgccagc
    49801 gttgttgcct gggcctcggt catgttgccg ataaccataa actctggtcg agcccctgat
    49861 tttaaggcgt cgcgataagc cagcacctct ttcaacgtaa tggagggcaa aattttacgc
    49921 cgttcatctc gcgagaagta cggcacttgc gagagcatct gcgcgggcat aatcgcctgc
    49981 tcaaacgctt tacccttttc tgcggaatcc atcatctggt tataccagga cttcgcctgc
    50041 tcaagctgat cttccgtagc ggtatagcta aagtacccct cgagcaatgc ctggaacagc
    50101 tgcggcagac gctgggggta accattagca ttaaccataa ggccgttgtt agcgttggtg
    50161 gaaaaactta tgccaccaac cgacgcctgg ttgcttaact gatcaagcgc cagccctgcg
    50221 agataatcat tgagcgcaaa catcacctga ttgcgggcgc tgtccatggc tttcggatta
    50281 cgcaaaatca ggctgacatc agctttgggt tcgctggaaa aataacggct tggcgcatac
    50341 accacgcgca gattcgactc atcaacaatc agctctggat ggtcatattt cttctctgac
    50401 ttaatcagcg agaaatcgtc aggaatatag gggttaagct ccggtaagga gagcgcaata
    50461 ttggcagctt tttgctgcca gtcagcgaaa gtttgttcgc tgattttatc gacctgatac
    50521 ggcgcatcga caaaataagc cgttttattg tgcggctctt tcgggctgat ataccagata
    50581 cgcgcattct gcggcgtcat catcgccaga cgttctttta ctgctttagc atcgtaccga
    50641 tcggcaatat tgactgcatc cagcgtatgc tcaaccggaa cgcgaatcat ggtatctgcc
    50701 agccattcga cgtaatccat atcacgggtg atcgacggat aacggaagtc gatatccagc
    50761 acattcgcca gttcatcgaa gtattgttta tcaatgcctt tttcacgtaa cagattgaga
    50821 tagctaaaaa ttgccgccac aacctgatcg cgattagcca ggcctttatc ggttaaagac
    50881 gcagagatcg ctaatacgcc gctgttgccg ttgacgatag gatcggagtt ggcgctaatg
    50941 ccctcaacta atccctgctt ttgcagccag tcggaaagtg tacctgggct gcgattacca
    51001 atcagatagg taatcaattc atccgtttta ctacggaact tcgctgagtt gttatcgatg
    51061 cgaaactcaa cgcgcagaac tttacgcggc attgccggga cgtaatgaat gataatgccc
    51121 ttttgcgcgt cggtgactac cggcacggtg atttccggtt ttttgctctc tttgttcggc
    51181 acgcgaccaa aggtgtccgc cgccattttt gccaactccg gcagcggttt attgctgtaa
    51241 ataaccgcct tcatcagatt ggcggaatag tacttctcgt ggaaatcttt cagcgcctgc
    51301 tgcaccggat taccaggttt atcgcttaaa gtttcgaggt taccaccaga aaactttgaa
    51361 ccggggtgtg ccgggttaat ggtttctgcg ctgacctgtg ccatgcgcat cccgtcacgc
    51421 gtacgcgcca tggttaattc agcgttcacc gcattacgct cacgttcggc atatttcttg
    51481 tcgagcaaag gttcagcaat agcatcggcc aggcggtcta ccgcaccagg caaggcgtcg
    51541 ttctcaactt ccagatagaa agccgtgcga tacggcgcag tgctggcatt gtgactaccg
    51601 ccgtgcattt tgagatattc ggccagactg tcagcctgcg ggtacttttt cgaccccatc
    51661 agactcatat gttcaaggta atgtgccagc ccctggtacg cctcgggatc ttccagcgac
    51721 ccaacgggca ccaccagcgc cgagagcgat ttaactgcct gcggatcaga aaccagcaag
    51781 accaccatac cgttatccag acgtatagcc tgatactggc ggttatcttt atcactttta
    51841 cggatggttt cctgaatcgg ctgccatccc gtttctgcct gacttaaggg tgcccaaagg
    51901 gcaactaaca acaataatgc tttgaaccag gtgctgcggg gcattctcgg acctcataat
    51961 caacttaatt ttctgtccag attcaacacg ttaacgcaaa tcatctgcca gaatttaatc
    52021 ttgtgctgca cgagtcagcc tatgtttata taaccatcag tccgtgactg gtgcgcatca
    52081 taaagtaagc ggatagattg cgcaattttt atacagcact catgactgat taaagcgaaa
    52141 cagcggtaac aggaaacgtt gcgactgttc aacgatggcc tccattgtct ctggtgttaa
    52201 ttgccgccag agcctttgat accagatatc atcaccttcg ccacgcacca tcatgttgcc
    52261 ttcgtaagcc tgaaggaatt tcgtacgggc tttttgcaac gtggaatcgt catccagcat
    52321 ggcatcgttt tgcgcgtcat aacaggtttt tagccacgcg ccgccacttt caggtaacac
    52381 cagcaatggc gcggacatcc cttcacgata cccctcaatc agttgtgaga ggtaatgcaa
    52441 agcctgttcg gctgcaagcg gcggaaaacg ccactcgccg tctttgcgta gaaaaaggcg
    52501 actttcacca ttaccaccgc tggcacagta gacaaggtgt tccagccaaa gttgcattcc
    52561 ctgcgccaca cttaataaag agggacgcca gcgcaacagg ccatccggct gcacctgcgg
    52621 caaccagcca gttatctgca caccgttgca ggcgagatca atttccatac tctgccccgg
    52681 ctggcgacag gcaatgactc tgtcggcaag ctgctgcatc tcctggcact gagtttccca
    52741 gaaaatttca ccaaaagcgc catacggtaa atcccctgcc gcccggaagc gacggaacaa
    52801 gcgttcggca tcatcctgct caaccagtgc attcaataac tgctgattga tttgataacg
    52861 gctaagccct tccagaataa atggctcggt gtcggggatt tcgctgtctt cagtacggaa
    52921 gttcacctgc aaacgcatct ggaagaacgc ccgcaccgga tgtgcccaga atcgttgtag
    52981 cgtttccagc ggcacggttt ccggtaaggt aaacggcagc ggctgaacaa attcagaatg
    53041 tgctttacca gcctggctgg ccgcaggtag ccattcacga gcatagcttt gtcgttcgcc
    53101 tggctgatag ttttgtggat caaacggcat tcgggtatgg aggcaagtaa gatgcgcttt
    53161 tacccttgcc tcgctttcat cacagttgag cgcttcatcg cccggtagat aatgactttg
    53221 cccgatgtag tcgatcagtt cctgcaccag taccgacggg aaacgttcac tgttatcctg
    53281 aatggaacgc ccgatatagc tgatatagag tttttgctgc gcggaaatta acgcttccag
    53341 gaacagatag cggtcgtcat cgcgacggct acggtcgcca cgcttcggtt tctggctcat
    53401 caggtcaaag cccaatggcg caagctgacg tggataaacg ccgtcgttca ttcccagcag
    53461 gcaaaccact ttgaacggaa ttgaacgcat tggcatcaga gtacaaatgt taaccggtcc
    53521 ggcgagaaaa cgctggctga tacgttcttg atccagacgc tgtgccagtt catcacgcaa
    53581 tagtgacagc ggcaccgcgt cgccatactg cgcacctaaa ccttcggcga taatcgcctg
    53641 ccattgttgt tcgatcagcg tcatcgccgc ttcggtttcc gcatccggca ggaagaaggc
    53701 gctgagcata tcgcgacaaa ccggcaacca ctcttctagc ggacgctcct gtgccagccc
    53761 gcgacgccag atgtttagct gcattagcag tgaagccaga tgccccacca gttctgcaat
    53821 taagccgctc gattcatcat aaggtagaac cgattgccac tcgccctgcg cgctctccat
    53881 cgcgtagccc agcaacatac gcgtcaggcc aaatcgccag gtgtgttgtc cggtggcggg
    53941 gagttccagc tcgcgaacgt tgtcgtcatc tatcccccaa cgaatgccgg attcgttgac
    54001 ccactggcgt aaataacgca gcccttcttc ggtgatgtca aaccgcgccg ccagcaccgg
    54061 cacatccagt aatgccagca cgtcttccga cacaaagcgg ctgtctggca acgataacag
    54121 gctgataaac gcttccagta ccggatgcga ctgccgcgcc cgacggtcgg aaatggcgta
    54181 aggcaggtaa cgatccgcag gtgcgctacc aaacacagcc cgaataaacg gactgtagct
    54241 gtcgatatca gccaccatca cgatgatgtc gcgcggacta agtgtcgggt cttcttccag
    54301 catcgccagc aggcgatcgt gcaaaacttc aacttcacgc tgcgggctat ggcaaacgtg
    54361 gaaggtgata ctgctatcca gtggatcaag cgggcgtttg ttatcgctac gggaaaactc
    54421 ttcgatgttc acaccagcaa cggcacggtt ttccagttcc agaatgtcag actgaatgtt
    54481 atgcagcagg ttatctggcg tgacatcgac aaaggcgtcc agctcctggc tgctctccag
    54541 gtcagaaagg agataaatgt agtcgcgccc aagcttaccc catgaagcca gcagcgggtt
    54601 gccgacatcc tgttcaccat cgctgttaaa gagctgcccg gcattttcgc tatcacgaaa
    54661 tagcggcaat tcgcgatctt caaaactgtg tcgacgctga cgggtcagca gtttcgccag
    54721 ataagcagga tctttaatat cgccccagta ataacggcag gggttggtaa acaggagatg
    54781 gatttcaata tgtttaccca gcgcctgtag cgcctggaga taaacaggcg gtaacgcgga
    54841 aataccgcat ataaagacgc gcgaaggtaa ccccggcggg caggtcgtcg cggactccag
    54901 cgtttcgata aagcgctgat agagattggc gcggtgccag cgcggttgcc cgagttgatg
    54961 ggtatattcc accagcgcct tccacaacgg ggcttgccag gcctgtgctt ctcccagccc
    55021 ttcaaccaaa tgtcctgttt cccactgtgc cagccagtcc ggacgataga ccagatactg
    55081 gtcaaacagg tccgccgctt ttgaggaaag ctggaacagt tttcgcttgt cgctatcgtc
    55141 agtcagataa tgccgcaaca gggtaaagtc ttcgcgctcc agcaattgcg gcagcagagt
    55201 catcagtttc cagctcatgc tctgtttgtt aaaggcgctc tctttgggga tttccggtaa
    55261 cacccggacg aacatatccc agataaagct cgctggcagc ggaaaatcaa tgtttgccgc
    55321 aatactaaac ttttgcgaca gggtcatttg cagccactgc gccataccgg tactttgcac
    55381 cagaatcatc tctggttcga aaggatcgtc cagccgttcg cgttcgacaa taaactccat
    55441 caacgcttcc agcacgtcca gacgattgga atggtagacc cttaacatag cggctcctga
    55501 ctactgacga ttcgggcaat gcaggcgggt catctcgcct tctctgcccc cgggtgaaac
    55561 tagcgtaacg ctgatgctga cacatcccgc ctgcgatgtc tgcattcggt tgacctgcca
    55621 gttggcaggt ggcgaaatcg cgcgcagttg cgtttgctgc caaccatgcc gccagagctg
    55681 ttggtactgg tttctgctgg cgagactgtt cattaatgtt cgctggatac ccgataacgc
    55741 agtgacaatc atcaccatca gcaccatcgc aaccattacc tccggcaggc taaagccttg
    55801 ttgattcttc agggaagctg acataacgcc ctctctttca gcggacaaaa atcgctccag
    55861 ccgcgtggcg aaaaaacaat gtttccatca atgacttcgc ctgttcgcca caacgaaacg
    55921 ccttcataac cggcaaccaa taaggcttca ttatctgcca gtaaacgcaa acaaacccgg
    55981 gcatcggttc cagcgtactg cgagcactga actgcgggct gcgtctgcca ggagtgcatt
    56041 ttcccccacg ccagcgccga ctgaacgatg gcctggcggc gcaatgactg actttctatg
    56101 ctcacgcgag aggcaaaact gcgatcctgc tgactcattc cttgcaatag caagctaccc
    56161 aaaaccagca gcatcaggac cagagccagt gacgaaacac ctttttcgcg gttcacaggt
    56221 tgaatcctgt cacgctatag ctggcatcca ccacggtttg cggttcagac ttactggcag
    56281 cacgcatatt aaccgtcaac accggcgaga agccgctgac atcctgacgt acgacctgaa
    56341 aagtgtcgat aatgatggca tccggattag tgactttatc ccagccctta ccttcacagg
    56401 atgtcgcacc gcgtagcgtt tccagcacat gctccttcag acgaaatcca atctggtcag
    56461 actcttttac cggttcgcga tcccagatac cgttactgtt cgcatcccac tgcacaatga
    56521 tacagtcacc ctgtccgaca atttccagcc cttcgccggt acagctgcca tgacaataac
    56581 ccgccctctg gagatgcttc gcgacggtaa atacccgcag ccagatttca tcttccagcg
    56641 ccagcttacg ggtgctcgtt aaactttcac gctgtaacgc aggcagaaag cgtgcagccc
    56701 ccagcaacaa tacgctactg atcgccatag caatcaacac ttccagcaga gaaaaacctt
    56761 gctcttttac aggcatcctt ctgtttctcc ttgctgacaa agccggagtc ttccccacgg
    56821 cgaaaccacc agccaccact cgcccgttga gtttttgaag cgaatatgcc cggcccatgc
    56881 ggtattgcgc aggccaaaga aagcaagcga aggtgtcagg tcgctcattt agacttcggg
    56941 ccagcgtggc acaaagacca atggtgaact gccatgacag gtattggccc cagcagcgga
    57001 actcacaagg caccataacg tcccctccct gataacgctg atactgtggt cgcggttatg
    57061 ccagttggca tcttcacgta aatagagcaa atagtcccgc gcctggctgg cggtttgcca
    57121 tagccgttgc gactgctgcc agtattgcca gccatagagt ccacttgcgc ttagcatgac
    57181 caaaatcagc atcgcgacca gcgtttcaat cagcgtataa ccacgttgtg ttttcatgcc
    57241 ggcagtatgg agcgaggaga aaaaaagacg agggccagtt tctatttctt cggcgcatct
    57301 tccggactat ttacgccgtt gcaggacgtt gcaaaatttc gggaaggcgt ctcgaagaat
    57361 ttaacggagg gtaaaaaaaa ccgacgcaca ctggcgtcgg ctctggcagg atgtttcgta
    57421 attagatagc caccggcgct ttaatgcccg gatgtggatc gtagccttca atctcaaagt
    57481 cttcgaaacg gtagtcgaag atggattcgg gtttacgttt gataatcaac ttcggcagcg
    57541 gacgcggttc gcggcttaat tgcagatgag tttgatccat atgattgctg tacaggtgcg
    57601 tatcgccacc ggtccagaca aaatcaccca cttccagatc gcactgctgc gccatcatat
    57661 gcaccagtaa cgcgtagctg gcaatgttga acggcaggcc gaggaagacg tcacaggagc
    57721 gctgataaag ctggcaagag agtttgccgt ctgccacata gaactggaag aatgcatggc
    57781 acggtgccag cgccatttta tccagttcgc ctacgttcca cgctgaaaca ataatgcggc
    57841 gggaatccgg gtcgtttttc agctggttca gtaccgtagt gatctggtca atatgacgac
    57901 catctggcgt tggccaggcg cgccactgtt taccatacac tggtccgagg tcgccgtttt
    57961 catcggccca ttcgtcccag atggtgacat tgttttcgtg tagataagca atgttagtgt
    58021 cgccctgcag aaaccacagc agttcgtgga tgatggaacg caggtggcaa cgtttagttg
    58081 tcaccagcgg gaatccatcc tgcaggttaa aacgcatctg atgaccaaaa atggaaagcg
    58141 ttccggttcc ggtacggtcg tttttctgtg tgccttcgtc gagcactttt tgcatcagtt
    58201 ctaaatactg tttcatggtt cctcaggaaa cgtgttgctg tgggctgcga cgatatgccc
    58261 agaccatcat gatcacaccc gcgacaatca tcgggatgga aagaatttgc cccatgctga
    58321 tgtactgcac ccaggcaccg gtaaactgcg cgtcgggctg gcggaaaaac tcaacaatga
    58381 tgcgaaacgc gccgtagcca atcaggaaca agccagagac agctcccatt gggcgcggtt
    58441 tacgaatata caggttgagg ataataaaca gcaccacacc ttccagcagc agctcgtaaa
    58501 gctgtgatgg gtggcgcggc agcacaccgt aagtgtcgaa aatggattgc cactgcgggt
    58561 tggtttgcag cagcaaaata tcttctgtac gggagcctgg gaacagcatg gcaaacggga
    58621 agttcgggtc aacgcggccc cacaattcac cgttaataaa gttgcccaga cgcccggcac
    58681 caagaccaaa cggaatgagt ggtgcgataa aatcagaaac ctggaagaag gaacgtttag
    58741 tacggcgggc aaagataatc atcaccacga taacgccaat caggccgccg tggaaagaca
    58801 tgccgccgtc ccagacacgg aacagataca gcggatcggc cataaactgc gggaaattgt
    58861 agaacagaac ataaccaata cgtcccccga ggaagacgcc gaggaagccc gcatagagta
    58921 agttttcaac ttcatttttg gtccagccgc tgcccggacg attcgcccgt cgtgttgcca
    58981 gccacattgc aaaaatgaaa cccaccagat acatcaggcc gtaccagtga agcgccacgg
    59041 gtcctattga gaaaatgacc ggatcaaact ccggaaaatg cagatagcta ctggtcatct
    59101 gtcaccacaa gttcttgtta tttcgctgaa agagaacagc gattgaaatg cgcgccgcag
    59161 gtttcaggcg ctccaaaggt gcgaataata gcacaagggg acctggctgg ttgccggata
    59221 ccgttaaaag atatgtatat gatccgcgct ataaccctcc gcgaatcagc ccgcccatgc
    59281 cgcgacgctc cataaaggct gcaacctgat ggcgaacttc ggtcgccagt tgcgcttcca
    59341 gactacgctg cgcaagattt tctgcttcgg cataatcaat gcgccgcagc aggtatttta
    59401 cccgcgctac agaacgtccg ttcatagaca gatggcgata cccaagccca atgaggattg
    59461 ccacgcacat gggatcgccc gccatttcac cgcacaaacg gagatcgatt ccatgtattt
    59521 ccgcttcccg ggcgatcatc gccagagctc gtaacattgc aggatgaaga ctgtcataaa
    59581 tgttcgccac ccgggtattg ttgcgatcaa cggccagaat gtattgagtc agatcgttgg
    59641 tgccaacaga gatgaaatcg acccgctttg ccagatgcgg cagcataaat accattgacg
    59701 gcacttccag catgatgccg atacgtggtt tgggaatttc gtaaccgatc atctcctcga
    59761 cttcacgtcc ggcacgttca atcaggcggc gtgcttcatc aacttcatcg aggcttgtga
    59821 ccatcggcaa cagaatattc aggttgcccg tagcggcatt agcacgcagc atcgcccgca
    59881 cctggatcaa gaagatctcc ggctgatcga gcgtaatgcg aatcccacgc caacccaggc
    59941 atggattctc ttcgctgatc ggcatgtaag gcagctgctt atctgctccg acatccagcg
    60001 tacgcaaggt gacgggttta tcattgaaca tttgcagcat cccctgatac tgcgccacct
    60061 gttcttcttc cgacgggaaa ccactttgca gcatgaatgg gatttcagtg cgataaagac
    60121 cgatgccatc aatacggctg cccagttttt cttcatgttc cgggcttaaa ccagcattga
    60181 gcatgacttt tatacgctca ccgcttttta actgcgcggg taaattgacg tcatcttccg
    60241 ccagacggct aagctcaatc tcttcactaa ttagccgctg atattcttgc agcagtaccg
    60301 gctccggatc gaccagcaat tcaccgcgat agccatcaac gatcagcgtc ctacgatgca
    60361 gcaccgaagg ctgaatatcc gcgcccatca cggtagggat ccccagcgca cgtaccatga
    60421 tcgcagcatg ggagttggct gcgccatctc gcacgacaac accgactaag cgatcctggg
    60481 gcagctcagc aagagttgtc gctgacagtt catctgccac cagaatgaaa cgttccggcc
    60541 aggcgttcgg cccttgatta gcgtcatcaa gatgaaacag caatcgctga cccagcgcac
    60601 gtaaatcgcc agcccgctct ttgagatagt tatcgcttag cgcggcaaac tgttcggcaa
    60661 atttttcaat gaccgttttt accgcccact ctgccaccga gcctttatca acctcggcaa
    60721 acaattcgcg acgcagccgg gtgtccgaaa gtaggtgcga gtaaagatcg aaaatagccg
    60781 ccgtttcttt ttgtgctccg gcggcaaagc gtttgctgta gcggcgaaac tcgtttgccg
    60841 cttcttccag cgccccggtc agtcgttcgc gttccagagc cggatccagc gttgatgcct
    60901 gatacacctg ttccattaaa ggtaacgtgg catcctgcca gccttcggca atcgccacac
    60961 caggtgctgc cggtaatgcg cggattcgcg tctggcgata ttgcccaaac aaggcagtca
    61021 actgcgactg agaaagaata gctgccatct gggtggcaag cgtcaccagg aaggattctt
    61081 cactttcgtc atactggcgc aactctcgtt gctgtaccac cagtacacca agcaactggc
    61141 gacgttgaat aattggtacg cctaaaaacg cgcggaaacg ttcttctttt acggagggga
    61201 tgtatttgaa gctggggtgc ttttgcgcat ctgcaaggtt tatcggttcc gccagcctgc
    61261 caaccaggcc gacgatccct tcatcaaacg cgagcgttac agtgcgaccg cgtggttttt
    61321 tcagcccccg ggtcgccatc aggtagtaac aacgtcgatc atgatcggcc aggtagaccg
    61381 aacagacctc ggtatccatc gcaagacaga tgtcggtaac cagaatattt aacgcctcat
    61441 tcaggcgtgg tgcgctggct accttttcga ctatttcgcg caggcgagtg agcataattg
    61501 gcgtgactta acctctttta cgtcgataag cagatgtgtt ttgtggtttt ggcgtatttt
    61561 cctgcagtga catcaccaca ctcgcgaact ctttcattac cctacggtag acatcacgtt
    61621 taaatgacac cacctgtctg accggatacc agtaacttac ccatcgccag ccgtcaaact
    61681 ctggtgtact gctggtttgc atattgattt ctgcatcgcc gctcaccagc tgcaagagaa
    61741 accatttttg tttttggccg atacaaaccg gcttcgtgtc ccaacgcacc aaacgtttcg
    61801 gtaatttgta gcgcaaccag ttacgcgttg aagcaaggat tcgaacgtct ttgcggctta
    61861 atcctacttc ttcaaacaat tcacggtaca tcgcctgctc tgcggattct ccggggttga
    61921 ttccgccttg cggaaattgc caggagtgct gaccaaatcg ccgggcccac attacctgcc
    61981 cctggcgatt acaaatcacg atacctacgt ttgggcggta gccatcgtca tcaatcaccg
    62041 gactacctca aaataaagct ttatatacga atgattgttt catactccaa gaagacggta
    62101 aaccactctc tgcagggcat tacacactaa taacaattga ataactcaca gttatgtgca
    62161 gagttataaa cagaggaagg ggtggatagc cgtttttatt caccttttct gtggatagag
    62221 ttgtgaagaa ctacggaatt actacgggaa aacccggagc attctgaata agcccgatat
    62281 agacatttta aaatattcat tattagtcaa tgcattacat gtaatttgag taatgaaaaa
    62341 gagtaattcg tgacccaggt cacacctctc atttacgggt tggcgaaaga tcaaccaatg
    62401 ccgtatttat ccacagaatg tgccactaag ttaagcactg aaccactaaa aactggagtt
    62461 tcgtcgcacg tcaaggctgt aaatggaaac agtagtggag gtttttcaca gttatcccag
    62521 ctttctgtgg ataacatggt gtaagatcct gtttattttc agtgaccaga tttggaaaac
    62581 ctattgcagt gttgcgcaac tcgattaccg gcagcctaaa aagcaatata aatcagagaa
    62641 ttgaacaaca catgtggaaa aagttacact gcgaatattc ggcacataat tgctgtttgt
    62701 tttttaacca aggtatcatg acatgtccca acctcgccca ctcctctctc ctcccgaaac
    62761 tgaagaacag ttgttagcgc aagcacagca actttctggt tatacattgg gagaactggc
    62821 ggcacttgcc gggctggtta cgccagagaa tttaaaacgc gataagggct ggattggcgt
    62881 gttactggag atctggctag gtgccagcgc agggagtaaa cctgagcaag attttgctgc
    62941 tctgggcgtg gaacttaaaa ctatccctgt ggatagtctt ggtcgtccgc tggaaacaac
    63001 attcgtttgt gttgccccgt taacgggtaa tagcggggtg acctgggaaa ccagccatgt
    63061 gcgccacaag ctcaaacgcg tactgtggat accggttgaa ggtgagcgca gcatcccgct
    63121 ggcgcagcgt cgcgtaggat caccgttgct gtggagcccg aatgaagagg aagaccgaca
    63181 gctacgcgaa gactgggaag aattaatgga tatgattgtt ctcggtcagg ttgagcggat
    63241 caccgctcgt cacggggagt atttacagat acgaccgaaa gcagcgaatg cgaaagcgct
    63301 taccgaagcc attggtgccc ggggcgaacg gattctgacg ctgccacgcg gcttttattt
    63361 gaagaagaat ttcaccagtg cactactggc ccgtcatttt ctgatccagt agccatcgct
    63421 ttgacctgcc gctttccggg catataatta ccgcttcatt tttttggcag ggctttttag
    63481 atgttatttg catggataac cgatcctaac gcctggcttg cgctcggtac gctgacgctg
    63541 ctggagatcg ttcttgggat cgacaatatt attttccttt ctctggtggt ggcaaagctc
    63601 cccacagcac aacgtgctca tgcgcgccgt ctggggttgg cgggagccat ggttatgcgt
    63661 ctggcgctgc tggcatcaat cgcctgggtt acgcgcctga caaatccgct ttttacaata
    63721 ttcagtcagg aaatttccgc ccgtgatttg attctgcttc tgggtggctt gttccttatc
    63781 tggaaagcca gcaaggaaat ccacgaatcc attgaaggtg aagaagaagg gctgaaaaca
    63841 cgcgtttcat cattcctcgg cgctatcgtg cagattatgc tgctggatat tatcttcagc
    63901 ctcgactcgg tgattaccgc tgtgggtctg tcagatcacc tgtttattat gatggccgcc
    63961 gtggtgattg ccgtaggcgt gatgatgttc gccgcgcgct cgattggtga ttttgtcgaa
    64021 cgccatcctt cggtaaaaat gctggcgctc tctttcctga ttctggtcgg ctttaccctg
    64081 attctggaaa gtttcgacat ccacgtaccg aaaggttaca tctacttcgc gatgttcttc
    64141 tctattgcgg ttgaaagcct caacctgatt cgcaacaaaa agaacccgct ctgatactcc
    64201 gtacgctctc ctgcgggagg gcgtactcac tcctgttacc ctcacagttt aagattttcc
    64261 tgctttcaag atatataacg tcggtttata aacagactat tatcataggt gggccaaatg
    64321 aggataaccg gatgaaaaaa tgggcagtaa taatttctgc agtcggactg gcgtttgctg
    64381 tttccgggtg ttccagtgat tacgtcatgg cgaccaaaga tggccgtatg attttgaccg
    64441 atggaaaacc tgaaattgat gatgataccg ggctggtgag ttatcacgat cagcaaggta
    64501 acgcgatgca aattaaccgt gatgatgttt cgcaaattat tgaacgttaa caaataaggt
    64561 cagcatccgg ctgtaatgcc gatcagttaa ggatca
//
LOCUS       DAESHI010000009        62625 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.009, whole genome shotgun sequence.
ACCESSION   DAESHI010000009 DAESHI010000000
VERSION     DAESHI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 62625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62625
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.009"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            912..2066
                     /locus_tag="KW011_000582"
     CDS             912..2066
                     /locus_tag="KW011_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBH7659009.1"
                     /translation="MPSPRIRKMSLSRALDKYLKTVSVHKKGHQQEFYRSNVIKRYPI
                     ALRNMDEITTVDIATYRDVRLAEINPRTGKPITGNTVRLELALLSSLFNIARVEWGTC
                     RTNPVELVRKPKVSSGRDRRLTSSEERRLSRYFREKNLMLYVIFHLALETAMRQGEIL
                     ALRWEHIDLRHGVAHLPETKNGHSRDVPLSRRARNFLQMMPVNLHGNVFDYTASGFKN
                     AWRIATQRLRIEDLHFHDLRHEAISRFFELGSLNVMEIAAISGHRSMNMLKRYTHLRA
                     WQLVSKLDARRRQTQKVAAWFVPYPAHITTIDEENGQKAHRIEIGDFDNLHVTATTKE
                     EAVHRASEVLLRTLAIAAQKGERVPSPGALPVNDPDYIMICPLNPGSTPL"
     gene            2217..3041
                     /locus_tag="KW011_000583"
     CDS             2217..3041
                     /locus_tag="KW011_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBH7659010.1"
                     /translation="MRLNTTGIAARMMLSLDKKAIGEKLINGRQINPTPLQVRYPQGV
                     REVLGIMSEQLAISTADLTRILLEDALHNMFMPADNTTGNIISRIEYIMLSHDINAPL
                     LATLLSPWNIRSTVIQDPARLADYLSADALEHLAECFNLNPDWLNGHENYPIALSGEW
                     PDTADNFRMLISDSSNTEVIFWHSFPFAGNTKREYCGVILRQKKEINGSVIYPALSLS
                     PTLLNDEKRKWLTEYTTRQNTTMSLRRVTLRPGLAGNLITGQILPVSLFNTSLLPW"
     gene            3126..4328
                     /locus_tag="KW011_000584"
     CDS             3126..4328
                     /locus_tag="KW011_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HBH7659011.1"
                     /translation="MMKKNHITRTIIASAVLFSFNAAAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKHNSNDDFSLLLPSVGAQVADPDDVSNKADDVKDDWDLFDSAVD
                     NQHGVQQAAANLKHRLQEFRNINADAQVGVSAVAAMANDTLPFALMVKSYGTVSVNGK
                     VNDADLDYLDKVANGTITDVDKNALTSRAFGRAAVITDVGISFAKELETAGQKWSLGV
                     TPKYQRVDLFNYNVTVRDYDKDDFDGDKYHNTKNGFNADIGAYTDLNDNWTVGLVAQN
                     IIPRSIDTKVVNGFKETFKVRPQATAGVSWHNDLFTTALDIDLTPASGFTSDSKRQFA
                     SVGAEFNAWKWAQLRAGYRQNMASNSGSAFTAGVGISPFDVVHIDVSGLVGTDHDYGA
                     MAQLQFTF"
     gene            4388..4972
                     /locus_tag="KW011_000585"
     CDS             4388..4972
                     /locus_tag="KW011_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBH7659012.1"
                     /translation="MMKPRSSYSKTAFILLFSVFLVAAVTKAKSSLPDITLEQAKEIN
                     ADNTVIFLFRHGERCDRSDMPCYSDKSGITITGTEKAQQEGIKFATIFSEYDIYSSNA
                     VRTIQTAKFFSGKEPVVMDSLSDCNNDLYKTLESIARESHKRNIVIMTHNHCLSFLAR
                     DRLGKKFKPAYLDALIMHYDGTRLILDGKYNKEA"
     gene            5367..5825
                     /gene="traH"
                     /locus_tag="KW011_000586"
     CDS             5367..5825
                     /gene="traH"
                     /locus_tag="KW011_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraH"
                     /protein_id="HBH7659013.1"
                     /translation="MNRIIPAGCLAALFLAGCNSPSQHTPVPRNYGAASDVAVTRHTQ
                     YTLWDKGVFSGQLPVSTGSHLLTANSDRLSFDWEGDAIELLNELARVRGMQFNYNGVR
                     LPLPVNLHVRDMTFSNTLRLIEAQTAWRATIHQYPGLLQVSFMQPENRKK"
     gene            5822..6640
                     /gene="traI"
                     /locus_tag="KW011_000587"
     CDS             5822..6640
                     /gene="traI"
                     /locus_tag="KW011_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraI"
                     /protein_id="HBH7659014.1"
                     /translation="MKALTSLLLPVILLTGCSSSPTISPGGVNTPPPDINAWLKPRSE
                     KPDSLSETRWKMLTDAGKTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISPEG
                     WLPPVIDEAQDVAHITPDQIRTSSRVWTIIRPERFVSNPPGWRDWLLRGLSTTATPGT
                     EGSVVPEDSVQRKVWETALRQGWQEGRQNADLTLEANQKTLTRDYRGMMLYSLLWRQG
                     MITRPDVSDQMQTVTGDGKKLVTGDRVRRLKNHAEFNLQKSHWRPLIGTEGGSR"
     gene            6637..7785
                     /gene="traJ"
                     /locus_tag="KW011_000588"
     CDS             6637..7785
                     /gene="traJ"
                     /locus_tag="KW011_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HBH7659015.1"
                     /translation="MNIPDEFGLFPFQRFTADELRHFFVWCAAHKVSDVDLTGGSPVS
                     VSRFGRRVRCSSATLPTTLMSSLIDELFGREVIPRVLAGNPVDRTIQINGDASGRYGL
                     KRGERIRLRSHLIQGTSGAEEKAISITMRVIPTEIPDILSMNIEPDLLEAMVCKSGLG
                     FVCGETGSGKSTLCSALYRYIMDNFPDAKIVTYEDPVEYILGNENDLLPPHQAEIGRD
                     VVSFAAGLRSAVRRNPEIIGVGEIRDNETADAAVQAGNTGHYCLSTMHTKSPGETLAR
                     LLGLFPPVIRDSMAWAVLSLLQFILVQVLVRTNDGGRKAVREYIVINDELRDNLSGMP
                     HAEWGHHIDAIIRQEKRRIRDQILEMYIRNEVDRREAILFIPPGELRS"
     gene            7782..8072
                     /locus_tag="KW011_000589"
     CDS             7782..8072
                     /locus_tag="KW011_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH7659016.1"
                     /translation="MKREGFWRYATPPVNIQGVPLPFTMIYLFICPFPSKTTFWICTG
                     IILFFVILDRSGWTVRTICTRIFSILRGAIASGRPWWYRHHTESPRDWTGLD"
     gene            8087..8638
                     /locus_tag="KW011_000590"
     CDS             8087..8638
                     /locus_tag="KW011_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HBH7659017.1"
                     /translation="MSGKKTIVTLLRVSLLACPLLFTTPSFAMIDTPSVKVGFSPEGS
                     ASALVLDTINSAESSIRMMAYSFTDPDVMHALAKAKKRGVDVRIVVDDKGNTNRASQE
                     AMKYINLLDIPLRTVDAFPIHHDKVIIVDGNTVETGSYNFSRAAARKNSENVVVLKNM
                     PDVAAQYLEHWQDRWNKGTDWKP"
     gene            8728..12495
                     /locus_tag="KW011_000591"
     CDS             8728..12495
                     /locus_tag="KW011_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBH7659018.1"
                     /translation="MPSVSRYRTWLAVPADEIEDLKKAHPPMNGHTPVLWDKEHKLWF
                     ARPGADLSMLDRWLPRPQEVSMNGSDPVTEFAQVLENAGLVLKELPAMDGKIHRVPTT
                     DDKNGQKSGAYRGFLDGRPAGWYRDYRSADDSPVNWTFSGGEQTDPRARLHLKAHSLQ
                     RREDAERELKAQYNRQAAYARRYVNKWPQATAHEYLTRKGIQAAPGVRINDKNELVIP
                     FRNRNGAIRSYQRIPVTGGKDARILKDSEKTGNWFALGTPRNGQPVLFAEGYATAASL
                     HEATGLPVLMTVDAGNMIAVAEDARQKWKQSPFIFCADNDHARQVNKGIVSATKAAEL
                     TGGSVIFPAFTDAEKAQGLTDFNDLDASRGRAAFQHVINAQLEHIGVSTPNSNTPEIR
                     EALVIGNLVFTPVHTEEKTMTPTEYPETSPDTGHSHDQGPSLPSATQQEQPTASSSNI
                     ADETLSFTSHATENNGKDERHPDNVQAQTESSVVQDSPAESPASPESTTASAPDEPAH
                     PAEPPEKVVSVATDKDWREFEAELSQPEKNELQQESGTSPEIPAPAAAPAAPEDSPSS
                     ETMNESAEPSPSPDAVKEEMTVMTEPDDKQSTGISPDTPREEAVSHVTEDIPLQPEPF
                     LPESDGPEEYEDYSAYQELMNNENPEHLQENSDMPSQPDTGPVQADESELQATTATIE
                     PAVRDEPTPQQPVQSTSPSDNTSSFLDKARGLFTRKKTDSQAHENSDPTPSAETTTTP
                     PIPDSIVYAPERSDAPISLNMDEILKELDWEKRADRTVLYKLDGKPAFIDRVNRLEMV
                     NGASNDDRSVLAALAVATKFYGGVIELTGSDAFKQKAMQLIIEHNIDVRMKLPDQRAE
                     LEKLRKEMAVSKDAVVTHQPTPELNRNTPEQPAVPDPVQEKEATQSPASPVAPEASTV
                     STASAPSPAEPGKTADVAPDEEPPGKLRPGESVTAVLHNFGRAEYAPGKGESFFVELK
                     NRSGSKLYWGEQLESLVKNHQKGDVVTLTLQNREQFILPGEQKARFRNKWSMESVTNG
                     ISVSHDNPDKGQRIQAIPVETFMKVAAQISQGWPEEMKALRMPENVGSHLFIGEDRHP
                     VSAPQNANQVTEITSAAPDKLTPVLGSVDKDTRELNLLLVQSADEHLQGVVRLNGTLY
                     PALATPSADNSQLVINALTDKGLRFAGYGEAVNHDADSTNRPAPELMQFHLKTREEPL
                     FAAVYTPEKQPDALYRNLGFEQSWQQWSNSQKPEDRQEKTLHQDLSHSPGR"
     gene            12513..12860
                     /locus_tag="KW011_000592"
     CDS             12513..12860
                     /locus_tag="KW011_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001629514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH7659019.1"
                     /translation="MNMHKSLVAVLGLILISSTASAATCRAGSAAQAGSNTGYERARA
                     AADAWSQRENDVSSSLQSCLSRIKKISINLPQFPSLDDILSQLENQVCDAVVDKVNEK
                     LPGNIDPWKDYNL"
     gene            12857..13549
                     /locus_tag="KW011_000593"
     CDS             12857..13549
                     /locus_tag="KW011_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HBH7659020.1"
                     /translation="MTENTEPSSPSHPAPDPEILKSTIRAMKQSEQRANNVPALIKAL
                     LCTGTCLLISITGNAIQYWHSTNVEREYFATDNGRLVRLAPTSQPAWSQNDAMAFGSQ
                     ALATAFNLDFVHYRSQISSLSPRFSDEGFAGYVNALQASNILETIKKEKMNLTATTGA
                     GVLVRQGQMSDGVWFWTFQYPVRMRLVGQTTSKPEQSFVFEITIQRVDPRLKPSGMEI
                     RQMISRNAGPNS"
     gene            13560..14543
                     /gene="traN"
                     /locus_tag="KW011_000594"
     CDS             13560..14543
                     /gene="traN"
                     /locus_tag="KW011_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraN"
                     /protein_id="HBH7659021.1"
                     /translation="MQSRYLLSTLLLVCSAATSADNAGWQNARTPQTTNSASDHVQNA
                     SQNTGGVPATLVKGELPAPGQASPLVQDAARLDSELSADEIRSLRSLMADNERAINAP
                     ITSVVPRISSLTVNLSPGASLPLVRTAMNNLSVVTFTDINGSPWPQSDPPYNAAPKLF
                     DVQYNENMVTITPLRPWASGNISVYLKGLSVPVILNVTSGETDTPSSSQEMDSRLDLR
                     IPRQGPTSPVVSIPTDKIALHDATLQAFLDGIPPRDPSVKRLKFTGNVPDTTIWQHGD
                     DLLVRSRAMLRDEFEQTLSSADGTHLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            14546..15835
                     /gene="traO"
                     /locus_tag="KW011_000595"
     CDS             14546..15835
                     /gene="traO"
                     /locus_tag="KW011_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HBH7659022.1"
                     /translation="MSAEQDAGKSGKKLAALLGLGSIILFGVGYIAFSKISGSNSDMQ
                     SAVNINSAASGGTRSVTETPHYRELLRADNERGAAAAARNNQTFIASLPQGLDIPDTP
                     EKQQQPAAKPENYAHRQASGTPQEDRAASEKRMERLQKLIVRIKDQHPAGSTPTIATA
                     MWNKSPAETTGQNGTQQFALQNASLSTPVAEKGIQLIPALTRIPAYIDTAVDSDNPSS
                     KVIATIPAGPWAGATLFSPGVKLVGNGVEIHFDRMSWNGMDLKVNAYAQREDNLMSSV
                     ASNVNTRWFKHIILPSVLGGVGSIGTLYKDANTQVIQGNYGTVTGRVGMPSGEAVAGV
                     IAGGMAERGSQILTRQAESEPYKQVEVYQHEVVSILFVDPVMTNDARSSSLSSGISPS
                     VNRTSQTEQRSQARMQTAMEQRKAVMQRRYDEQPETP"
     gene            15835..16539
                     /gene="traP"
                     /locus_tag="KW011_000596"
     CDS             15835..16539
                     /gene="traP"
                     /locus_tag="KW011_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraP"
                     /protein_id="HBH7659023.1"
                     /translation="MKPETEIDETGSFGEPEEKPAPFWKRSVWGISVATWGLCAVVLL
                     AAIWYLFLRAPSETGMPPFNDADAGVQTWQTTQESSPSVQSTETMTVRTGEDMSQLAR
                     DVKTELDNRDEKIQATLNMLHDSINKLGEAIKKDEEYAQETRRQLDDIRSHLNGMMTQ
                     KSVTESSSTPHPAKKKTSSVLNGMKIMSMETGMAWIRWQGSTWAVREGQTLGNVVIQR
                     IDPTTRTIITSAGTLR"
     gene            16539..17066
                     /gene="traQ"
                     /locus_tag="KW011_000597"
     CDS             16539..17066
                     /gene="traQ"
                     /locus_tag="KW011_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HBH7659024.1"
                     /translation="MNMDALTAIENFASSIFSAGMDFLFTWGEFIGVISMITLFARAR
                     SAGPVKMSPGKFIAGMLTSCMLVSLPAMINAGGVQMGFRADSFGPIAYVQPQTFGAAA
                     GAANAVLSLAKLAGVGFVMNGISIWRKAGLDGHTALSASESVSKGNVKFIAGVLLVFI
                     DRVLNALLASIGIVF"
     gene            17117..17521
                     /gene="traR"
                     /locus_tag="KW011_000598"
     CDS             17117..17521
                     /gene="traR"
                     /locus_tag="KW011_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraR"
                     /protein_id="HBH7659025.1"
                     /translation="MSRIRVVAARLYAAFIHAQLFVMSCRSKLTKFLLLIPMMLLPKA
                     VLADGDLADMVRNVEQGAKTAQSSSLTIAQFIGVILFLGGLIGLKKVGKQGGMGLAGC
                     IVSIVIGALLVAGPEMMSRSQKQLGISTISIG"
     gene            17585..17773
                     /gene="traS"
                     /locus_tag="KW011_000599"
     CDS             17585..17773
                     /gene="traS"
                     /locus_tag="KW011_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative conjugal transfer protein TraS"
                     /protein_id="HBH7659026.1"
                     /translation="MSAWSPEHITLRRTENGNVIAHDSRDDQEFIFAECCSDEIANII
                     LEAVKRYTSTEPAHATQH"
     gene            17757..18557
                     /gene="traT"
                     /locus_tag="KW011_000600"
     CDS             17757..18557
                     /gene="traT"
                     /locus_tag="KW011_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraT"
                     /protein_id="HBH7659027.1"
                     /translation="MQHSIKDLWLYPFPEIDVVHTQEPLLPEPELTTPGRCICCRQNV
                     RHRFRLDDSWPLRQLTDTISDTRVRLNKATEHLDKLKKRGEPVATGEKEKYNTAVKAA
                     ERALEQARLSARRLSLRHVQKAEITSTESLSEKEQELFHEDGPPYSLCAFCHAWHSLN
                     GYAAAQGVMVWLPDLHPSTVVALNRRSLQEVFSNDKFRVRRGREALSALMQNRLAVED
                     KFRSFRPADFADVFRRYPPSGRSPLREKMNGIALILTPDSFIKKEYVD"
     gene            18647..21691
                     /gene="traU"
                     /locus_tag="KW011_000601"
     CDS             18647..21691
                     /gene="traU"
                     /locus_tag="KW011_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraU"
                     /protein_id="HBH7659028.1"
                     /translation="MNINRLIFTIEDCLNTLSRFSVASSFVEYCDLRTVIGLDRQDRE
                     RRPWLNSPYIAATKRGEYLSVFEVSGAFREMDEASDQTGPGSLESLITSMSDSLNTAY
                     KNSGHKISFVFERDPDMGKEEIEDMVAPQKRSLANTGIQLQDVVDEKVTTLSPWLVRE
                     RCWLAIWSGPDLISNSDRTAHDELVRRLAERVPKARFAQSPWQWTLSALKIRHEAFLD
                     NVEQALRHSSDGLILRLLDIHEVGREIRRQTERHSTPRNWQPHLPEDAQPAGYRWTDD
                     ESVLHAPSLHLQLFNTQVTTQGNLVQAGGLWHGMVSITLPPQNLQTFNELVRAVPRAV
                     PWRIRMDLMPGGMKALNLKKTLLTYSSFISAVRPMYESVMTLAATDEKEPVCIMTIMA
                     STWGKTREICTRNQAILKSAIEGWGVCGTTTTFGDPRRAWVNTILAASGGSGPVPLYP
                     PLSHAISLFPLNRAGSVWRGKGNLMLHTEDGSAFEVGLASSQQNKHTELAPGDPGLGK
                     SVLINTLSEIQISSAQKNLPFIAYIDKGYSAQGLVQLIRDSLPPERKDEAVGIILSND
                     PEYTRNLFDVMYGAKKPITPEKNFMSSVLCALCVDTGTGQPCNPGDTRQIINQLIELA
                     FKEYGENNPRLYRASTEELVDSALQDSGLYEKHDATWWARSTWFEVRDMLHNAGYIMA
                     AQRAHYQAMPQLPEVSSMLGHTSLRDVFGTVQRDGSNELLLDYIRRALEQGHNDYPMI
                     SGYTRFMINPETRVIAVDLNNVAGDKTPAGRLKTGIMYLLAGQIAGGDFTLPQYRDEV
                     LKQLPREYHEIALKRINQLDQEVKTKVYDELHNARGIDFIWENLDTQEREQRKFAIRT
                     VLSTQYLRDYPESVLKSANTLWLLRYKPEDIPVLRDNFNVPEFMLKRFLKMPEGPAPD
                     GSGVPVLGVFRVKSGTLARILKFTVGPLELWALNSSPKDSALRKTLTNKLGSVRARKI
                     LAENFPRGSATSLIEHRAGQHNSDNVIEELASELIRKQGYNL"
     gene            21691..22305
                     /gene="traV"
                     /locus_tag="KW011_000602"
     CDS             21691..22305
                     /gene="traV"
                     /locus_tag="KW011_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraV"
                     /protein_id="HBH7659029.1"
                     /translation="MCTIHITPRCRRPARGQVFLKKLEVKGDSFSFTLPSRVFRLHPA
                     PNPRVIYASDARTQGKVTGMRLLLPSSTDELITTAIWQVSVPDEAPFDVQHVIQWILP
                     SPLASLMSDDTWLWPSVPGTPALSAGDWPVMDTDPSTLRRVRRRSFSIIDNRNRKGYI
                     TRRYQCFPLAPLPEDRKYDSLPDLVLHPEQETIKCKEKHYSQPL"
     gene            22272..23474
                     /gene="traW"
                     /locus_tag="KW011_000603"
     CDS             22272..23474
                     /gene="traW"
                     /locus_tag="KW011_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraW"
                     /protein_id="HBH7659030.1"
                     /translation="MQRKTLLAALIATLSGTACQAHAYSVTVVASRPVEEQVIPRMEA
                     IKDVLGNILSTQTATGTAINQNSEKLASVIAQNGQATRQQMIFSNETQRLEEARKSFT
                     VPDSICSESASGIATESKSASASAASKLSKGGGVSNRSIRDRLASAANSPVREAYDGA
                     AIHASYCTEAEYARFGGTAVCPSVGEIPGGDSQVRSIYHGAGTADTPAALTWDQKQID
                     AATAYMKNTSRPSAGRALGKGEVNTQSGRTYVGLQNEYNGIIDSASNPQLTLIADSTP
                     NESTRKALAETLQSDSAAAYFDQVASPEAKARGYMSTREFEAFEAGRRYANTAYLVDL
                     QEMQGDNLLRELVRITAQMNWQLNDLKEQIRQGNVISGQQLALTARQYYEKQLGSLEK
                     TINQANAR"
     gene            23503..24087
                     /gene="traX"
                     /locus_tag="KW011_000604"
     CDS             23503..24087
                     /gene="traX"
                     /locus_tag="KW011_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraX"
                     /protein_id="HBH7659031.1"
                     /translation="MNKLPENSDQTKNVKKSPSITRIATRGIINIFVPLMETRLITNS
                     VQHMLEQQKRRLDRFRQVNNKKAQLHLTWEEALQASHMSVDDLDRRFRRRRTVWRFCC
                     WSLLAIALFLSGMLFAASSLPLTTLVRAISTLVLILSGVALCASRALIVTYRLWQLHE
                     RKVSEPEQGTFRDFLNDRNGWRNATLIAVTSKQY"
     gene            24184..26352
                     /locus_tag="KW011_000605"
     CDS             24184..26352
                     /locus_tag="KW011_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HBH7659032.1"
                     /translation="MKILLRALCAGLAISSLPAMASVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLSTSAPTLIGSMFGAFNSIIAVLAVVWFMFIGIRHVVRSGHQGQVFST
                     GRDIVGTLSVVAGFLMIVPTGNGWSIAQLIMLWGASIMGVGSANVMVQLAADNIANGY
                     SMTVQPVQASTRTAARGIFEMELCKYAVNAGLNDFNQTVKSSTSLMTESAKTASGNYT
                     VTVSNGSGICGSASLSVEGNGTTDQSTIGKFFNPFSKNEYSGVISAQRAAMDNMISDM
                     DNAASEFVTTFLEKRNSGNGTLPDIETRIQRAADEYERAVQKSLPIDNGEQSRKEALK
                     SYLTTYGWVTLGAWYQTFATANQRLAELADRAPAVTSMSSLGEVGDTDLFSAVMGAYK
                     TQLQNSTYTPTIGTITTQDESNAANSTDPQSVVMKSIAPTILKWTNQAATEWSGTGTT
                     SNQVNPLIKMKNIGDYTLGTTEILWTGYTTVRVLATMGDNSIFGKAVNLFSGLPKGFV
                     ALLDAAAPPIYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCTAGPLWA
                     ATHLGTSEDRGSRAAYGYIYLIDSMIRPPLMVFGFFFASVAIIAVGTILNSLFGAALV
                     NVQVKSLTGIFSLAGFLLIYARMCTTSVAAIFALQAYLPDHVINFLGGRDGVNTLGNL
                     TSSVKDIFAGSNRNIRHSPGVREDRLKNVTKDDNKDGIKG"
     gene            26426..27076
                     /gene="excA"
                     /locus_tag="KW011_000606"
     CDS             26426..27076
                     /gene="excA"
                     /locus_tag="KW011_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HBH7659033.1"
                     /translation="MQMVKTINRYPAWWTLYYALRFIYFISIPVLSIFILFGVLSITS
                     SRYVTQEDYIYTCVCLFLLIAPAILMYSRASSRKDKIKKIVAEIKNTGFYSPDKEYEG
                     LSFTQGVYFGVDTKKGTMLYARAYPGNIMDIIGFDIDNFTRTVTDAKTLEIYTKYINI
                     PMVSIPSGCIHPKMMADTMHAMAERGYDYPVDFPRLIQEKRKEWEQIAGMPVAEVF"
     gene            complement(27148..27357)
                     /locus_tag="KW011_000607"
     CDS             complement(27148..27357)
                     /locus_tag="KW011_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBH7659034.1"
                     /translation="MSMPPAIANTFLFEMMKSKSKDVTLAAIYALGEGRCQAENITRE
                     LHRLSQSDDMEIKIAAIKALGRIYR"
     gene            27749..27925
                     /locus_tag="KW011_000608"
     CDS             27749..27925
                     /locus_tag="KW011_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659035.1"
                     /translation="MNLVDAFVKKVISEPYEEYGKWWIDVEYISWGVPGKTRLMFESK
                     EQALEVKEGYKFLT"
     gene            28584..28835
                     /locus_tag="KW011_000609"
     CDS             28584..28835
                     /locus_tag="KW011_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659036.1"
                     /translation="MSEKWSKEFDSCFQVGSIEEIADALEKRSGSEENALAMLAMTAF
                     TLMTRRGICEMQNVAPDGKGIRLELIGFRENETIPDTLH"
     gene            complement(28907..29059)
                     /locus_tag="KW011_000610"
     CDS             complement(28907..29059)
                     /locus_tag="KW011_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HBH7659037.1"
                     /translation="MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATL
                     AYEVKR"
     gene            29351..30559
                     /gene="trbA"
                     /locus_tag="KW011_000611"
     CDS             29351..30559
                     /gene="trbA"
                     /locus_tag="KW011_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HBH7659038.1"
                     /translation="MSYNRQPVAEDPMQIWGAVGVLLILLLFVIWLFLPEVVYASCLI
                     LHTLWGLVDWGPFHNYAAPRYNLLAMTGNNAANISYSQWVNVMEQTIGILWMYLLPVT
                     LWCLWEWYQHPGQSRFTRRPVDITRLPHIFASLSPAIAPVLADGDPEKLFHGGKRPER
                     RVALTPEAFVEQHTLITNMQLDVAAARRCFMAQLGKPLTSWKDMAPHEKALFAIFGLQ
                     YFLDDRKAALKLMDTLNLSCRIKSKRDSGKFCTPVYSLAKSAFQRVIKSNGAQQWLKQ
                     HRYVRSGLVWLYAHDLRLTPPNWIWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     EAEAMRFGLPCPEPCVDEAVEGLRRDMLSLGLIWDEPQPDRDRKRRILTNWSLTDDIL
                     PRTPATDNEF"
     gene            30578..31648
                     /gene="trbB"
                     /locus_tag="KW011_000612"
     CDS             30578..31648
                     /gene="trbB"
                     /locus_tag="KW011_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbB"
                     /protein_id="HBH7659039.1"
                     /translation="MTIEYFATRIAKHISVQAIWPDGRTEIIAVLPRDALSGLVTRNN
                     QLIHIAFDAMGTRNRETVLIDKQEHNADQILTAIDSCLRLEYLMERRFSRTPLFRGII
                     ASVVLFVMATIGFSLFRYVDRVFWDDTTPEAVQTAGEPRLLPPHLNHTVPLNEGIQLP
                     VPPKKDVQVPEKTITSKNPEAAAARHNLATVLKRNADRGMFTINLSSGHERTLFAFLD
                     PACPNCRLLEPALKRLASDFNVVIYPVSVIGGEESTDRVAPLLCEKDAQKRAAGWHRL
                     YSADNGMMTPSEETTPADETCLKAARAAIDVNNVAFRKFGFAGTPWVLSDTGWHLPTG
                     ILQETGTLNLFLKTTDSESGHE"
     gene            31641..33932
                     /locus_tag="KW011_000613"
     CDS             31641..33932
                     /locus_tag="KW011_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HBH7659040.1"
                     /translation="MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIW
                     PLALPACLLFTLITMLVFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQY
                     EVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINP
                     LCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTR
                     PQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTS
                     AHTTSALGRMFITQKSMILARDLGYRLEGTDSDALEVKKYKGRFPYLCFLDEVGAYYT
                     DRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLMQNTGTKFAGRIVSEGSTA
                     RTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELHPGEFFS
                     IFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPE
                     NVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESA
                     LWETAVNTLKTTTREERRIRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNH
                     PTGRPVNGFHHKKTNRPDWDGMY"
     gene            complement(33969..36668)
                     /gene="nikB"
                     /locus_tag="KW011_000614"
     CDS             complement(33969..36668)
                     /gene="nikB"
                     /locus_tag="KW011_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxase NikB"
                     /protein_id="HBH7659041.1"
                     /translation="MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQ
                     PHRSRFSRLVDYATRLRNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEY
                     IARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTD
                     TDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRI
                     VRKTAVERDRQNAWTRGKKQTFREYIAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQ
                     MDGKFLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNP
                     DFIAADVRPEKIAETESLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRV
                     QHGHLVICDGYDHNQTPVRADSVWSLLTLDNVNQLDGGWQPVPTDIFLQVTPTERFRG
                     RRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGYSISHCSPQIEEMITQGEF
                     TWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFV
                     SAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAW
                     REQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLK
                     EDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTT
                     DRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGEFVCTDYGDRVVFRNHHDR
                     NALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAWHNVTGRTGHEDYRITR
                     PDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG"
     gene            complement(36679..37011)
                     /gene="nikA"
                     /locus_tag="KW011_000615"
     CDS             complement(36679..37011)
                     /gene="nikA"
                     /locus_tag="KW011_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxosome accessory protein NikA"
                     /protein_id="HBH7659042.1"
                     /translation="MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSA
                     YIRNAALNKRINSRTDDAFLKELMRLGRMQKHLFVQGKRTGDKEYAEVLVAITELTNT
                     LRKQLMEG"
     gene            37245..37580
                     /locus_tag="KW011_000616"
     CDS             37245..37580
                     /locus_tag="KW011_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HBH7659043.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTASKKTDSAR
                     KDGIDMNNN"
     gene            complement(37665..38513)
                     /locus_tag="KW011_000617"
     CDS             complement(37665..38513)
                     /locus_tag="KW011_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HBH7659044.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKRIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRS
                     SIGAQYGEFIGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     SLPPRV"
     gene            38862..39059
                     /locus_tag="KW011_000618"
     CDS             38862..39059
                     /locus_tag="KW011_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659045.1"
                     /translation="MQGAPHPARLKKNDGATMACGGHWHTTPAQVKTEKTARHLQSKK
                     VKFLHFFTLIFRADLVVMVIK"
     gene            39093..39344
                     /locus_tag="KW011_000619"
     CDS             39093..39344
                     /locus_tag="KW011_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH7659046.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(39375..39545)
                     /locus_tag="KW011_000620"
     CDS             complement(39375..39545)
                     /locus_tag="KW011_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659047.1"
                     /translation="MTIAERLIQKGFEKGALEVAREIACRLRDMGWTPERIQEATGLS
                     GEELKKLFPDEQ"
     gene            complement(39607..40548)
                     /locus_tag="KW011_000621"
     CDS             complement(39607..40548)
                     /locus_tag="KW011_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBH7659048.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRQRDMLMCLDNLVRALQDIQDEEQITVLFNYL
                     LNGSEHVTVEFLQTLAQRLPQHEDSIMTLAERLKQEGIQQGIQQGIQQGIQQGVQQGA
                     LQKAREIARELRNAGMPAAQICQLTGLSEAELKNITH"
     gene            complement(40613..40879)
                     /locus_tag="KW011_000622"
     CDS             complement(40613..40879)
                     /locus_tag="KW011_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659049.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(40971..41405)
                     /locus_tag="KW011_000623"
     CDS             complement(40971..41405)
                     /locus_tag="KW011_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HBH7659050.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            complement(42134..42634)
                     /locus_tag="KW011_000624"
     CDS             complement(42134..42634)
                     /locus_tag="KW011_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBH7659051.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLATFDDERDFFAACRSL
                     HQDEADPELMFQDYEGFPGNMASECHINWAYVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDTFRDAWWGEADSEEAFAVEFASDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(43097..43693)
                     /locus_tag="KW011_000625"
     CDS             complement(43097..43693)
                     /locus_tag="KW011_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659052.1"
                     /translation="MMKSDEQYQVPVWMRPLLPLLCNTGGNDPEELLNDTETTASANI
                     VRYVLIVAVRSQIDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISKALSGER
                     MAARDRNADLTWIRQQLVDAAWFVRATLEAHGMGVGNESPSAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSSVVREAQDWLCDRKLVDITDILR"
     gene            complement(43690..44409)
                     /locus_tag="KW011_000626"
     CDS             complement(43690..44409)
                     /locus_tag="KW011_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HBH7659053.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            complement(44406..44840)
                     /gene="psiB"
                     /locus_tag="KW011_000627"
     CDS             complement(44406..44840)
                     /gene="psiB"
                     /locus_tag="KW011_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HBH7659054.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNCGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            complement(44895..46853)
                     /locus_tag="KW011_000628"
     CDS             complement(44895..46853)
                     /locus_tag="KW011_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBH7659055.1"
                     /translation="MPVTKCEPETTRKASRKSAKTQETALSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSVESVSELAGSIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERNILPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRALAQEGKTPAQTG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALESDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVKDNTKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAED
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDAADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPVQISLECKHYSLTSDAPSGKEGAAFLVLMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            complement(46912..47145)
                     /locus_tag="KW011_000629"
     CDS             complement(46912..47145)
                     /locus_tag="KW011_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HBH7659056.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDHGEHFRLV
                     VRNNGTMVWRTWNFEDGAGYWMNHAIRDFGILK"
     gene            complement(47203..47730)
                     /locus_tag="KW011_000630"
     CDS             complement(47203..47730)
                     /locus_tag="KW011_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBH7659057.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWDDNGITRYITEIL
                     VKTTGTMQMLGSAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     TPEGEDYGFSDDIPF"
     gene            48099..48167
                     /locus_tag="KW011_000631"
                     /pseudo
     CDS             48099..48167
                     /locus_tag="KW011_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus protein"
     gene            complement(48500..48691)
                     /locus_tag="KW011_000632"
     CDS             complement(48500..48691)
                     /locus_tag="KW011_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659058.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVV
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(48688..49110)
                     /locus_tag="KW011_000633"
     CDS             complement(48688..49110)
                     /locus_tag="KW011_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HBH7659059.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKRVSRQVTVPAVMLEKVVALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(49157..49582)
                     /locus_tag="KW011_000634"
     CDS             complement(49157..49582)
                     /locus_tag="KW011_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBH7659060.1"
                     /translation="MQYAKPVTLNVEECDRLFFLPYLFGQDFLYVEASVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHIVNGANWFDRTVSADACGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(50000..50770)
                     /locus_tag="KW011_000635"
     CDS             complement(50000..50770)
                     /locus_tag="KW011_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659061.1"
                     /translation="MNKTLNALVCRHARNLLLAQGWPEETDVDQRNPNYPGWISIYVR
                     LDAPRLATLLINRHGGVLPPLLASAIQRLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVHDAVRSICYQVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSCGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARTEIRSMADALAKIRK"
     gene            complement(50821..51255)
                     /locus_tag="KW011_000636"
     CDS             complement(50821..51255)
                     /locus_tag="KW011_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HBH7659062.1"
                     /translation="MYGTCEILCRELAAKYPADTPLMLVVWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRIESGISSGVAMEIISNVRENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDNGLAVPECVTRRQAVINQARTLLKNNTHENN"
     gene            complement(51267..51488)
                     /locus_tag="KW011_000637"
     CDS             complement(51267..51488)
                     /locus_tag="KW011_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659063.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(51489..52172)
                     /locus_tag="KW011_000638"
     CDS             complement(51489..52172)
                     /locus_tag="KW011_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBH7659064.1"
                     /translation="MSRFVLGNCIDVMARIPDNGIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYVLAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(52249..52560)
                     /locus_tag="KW011_000639"
     CDS             complement(52249..52560)
                     /locus_tag="KW011_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659065.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASLSPFSDERPSSRFRGIFSPSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(52557..53459)
                     /locus_tag="KW011_000640"
     CDS             complement(52557..53459)
                     /locus_tag="KW011_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HBH7659066.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRETITVHFTAKRGDWCDIWGSEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGDNCIQVDF
                     DTPWCQPESDVVAALSRRFGCTLEHWYAEQGCNFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            53877..54125
                     /locus_tag="KW011_000641"
     CDS             53877..54125
                     /locus_tag="KW011_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBH7659067.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            54122..54559
                     /gene="umuD"
                     /locus_tag="KW011_000642"
     CDS             54122..54559
                     /gene="umuD"
                     /locus_tag="KW011_000642"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBH7659068.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            54559..55833
                     /locus_tag="KW011_000643"
     CDS             54559..55833
                     /locus_tag="KW011_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBH7659069.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGHRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
     gene            complement(55835..56251)
                     /locus_tag="KW011_000644"
     CDS             complement(55835..56251)
                     /locus_tag="KW011_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HBH7659070.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(56244..57224)
                     /locus_tag="KW011_000645"
     CDS             complement(56244..57224)
                     /locus_tag="KW011_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HBH7659071.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            complement(57638..57946)
                     /locus_tag="KW011_000646"
     CDS             complement(57638..57946)
                     /locus_tag="KW011_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HBH7659072.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            complement(58033..58677)
                     /locus_tag="KW011_000647"
     CDS             complement(58033..58677)
                     /locus_tag="KW011_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBH7659073.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            complement(58857..59459)
                     /locus_tag="KW011_000648"
                     /pseudo
     CDS             complement(58857..59459)
                     /locus_tag="KW011_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            60017..60361
                     /locus_tag="KW011_000649"
     CDS             60017..60361
                     /locus_tag="KW011_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HBH7659074.1"
                     /translation="MKNLLDTEGTVLFPFQTEIHFIWTIFSTVKRLVIGTRDHICQKQ
                     YWSACLCILLLMAYVGLCAAVVWFVVPWLKAFIVSKFAVSALWVTPVKRGLLWGIGKV
                     VTATCRRFTLLK"
     gene            complement(60537..61538)
                     /locus_tag="KW011_000650"
     CDS             complement(60537..61538)
                     /locus_tag="KW011_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HBH7659075.1"
                     /translation="MADPFNLQTDVVRQHTVPRFLLKHFSTPGKGKRQRLYAFDKAAG
                     RAYATTPDDATVRNTFYNLDNHPDRLSLEPLLGIYEHHAAPVIAALLAHRDIRRLTDD
                     ERYRLAVFVAVQRARTFGELERISGMISVLTDKMEAIGSTKEQAMETLGLSSGGDTKD
                     IFLRQLVQQVSHIDLLLKKDWYLLETRPERPFYVSDNPVVLKNSNDFGPYGNLGLAVR
                     GIQIYLPLSSTLMLAMYCPSIREQMVRQKQHLQHLLARAPHLIPRHIRPFERLEHIRR
                     YTDYLLMPLTPEHVTHYNSLQVEFAEQYVFCGEKDFSLVERMLADSERYRTGPRFTF"
     gene            61889..62146
                     /locus_tag="KW011_000651"
     CDS             61889..62146
                     /locus_tag="KW011_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659076.1"
                     /translation="MDQEMTFSLSYEQLTRFAERRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRNLIWPEDDR"
     gene            62146..>62625
                     /locus_tag="KW011_000652"
     CDS             62146..>62625
                     /locus_tag="KW011_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659077.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVQLNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     "
BASE COUNT        15961 a        16639 c        15574 g        14451 t
ORIGIN      
        1 aggccagtgc caatccggta cttggcgtaa aagcaattct ggtagtacgg tgataactgg
       61 tcgtatagca aatggtcagc aaataccgct gccaacagga ttttctgcca gtcagtgctc
      121 atggagtgtg agcaatgcag aaaaccctca aggttggaag ccgaattact ttgcaggatc
      181 agttgcaact tatgatgcaa atcgaattgt taaatgtggt ttttatgatg aatacaattt
      241 ccataaaggg acatttagag ctgatttaac cggaaaatgt agttatgtcg tcgcgtgtca
      301 gaactgagat taaattgctg gcgctgctgg cctacgtgcc aatccggtac ctggagaaag
      361 gtaggatctg gtgagcttca aattgctaca gcacaggcca caggttggcg ctttcctgga
      421 gcaacagcta cttgtccaac tggaaaacgt gttacaggtg gcggcggaat atgcacctca
      481 cgaactggtt acatatggct taccaggtca ttcccatcag ccaataactc atggtcggct
      541 gcgtgcgata caaccgaaga tcagaatggt tcaataactg tatatgccat ttgccaataa
      601 gctatttcaa gcaagtaatc ttcataaagt aggacgaata ctgtgtgtag tttttggctt
      661 gccaagtcat ctcgccgtta gagtcaggca tgtcaatagg aacgacttct gtttctgcca
      721 tttcacgccc gtcaatacga taggtgttaa tacacagttt aaacctccct aagacctgtg
      781 tgtttttggg atataacgta aatgttttta tattcgtcca tatcgacgct gatgatgatt
      841 tccaggtacc ggattggcac gcttgcagag aatgattgtt ggcattattt tttgcctctt
      901 ttgaggttag catgccgtct ccacgcatcc gtaaaatgtc cctgtcacgc gcactggata
      961 agtacctgaa aacagtttct gttcacaaga aagggcatca acaggagttt taccggagca
     1021 atgttatcaa gcgatatccc attgcccttc ggaatatgga cgaaataaca accgttgata
     1081 ttgctacata cagagacgtt cgtttagcag aaatcaaccc ccgaacgggt aaacccatta
     1141 caggtaatac tgtacgtctt gaactcgccc ttctgtcatc tctgttcaat attgctcgtg
     1201 ttgaatgggg aacctgtcgt actaacccgg ttgaactggt tcgcaagccg aaagtatcct
     1261 cgggacgaga tcgccggcta acgtcttcag aagaacgtcg cctttctcgc tatttccgcg
     1321 aaaaaaatct gatgttgtat gtcattttcc atcttgccct tgagacagcc atgcggcagg
     1381 gcgaaatact ggccttacgt tgggagcaca ttgatttgcg ccacggtgtg gctcatttac
     1441 ctgaaaccaa aaacggtcac tcacgggatg ttcctctgtc cagacgtgcc cgtaactttc
     1501 ttcagatgat gcccgttaat ctccacggca atgtttttga ttacaccgca tccggcttta
     1561 aaaatgcctg gagaatagcc acacaacgac ttcgcatcga ggacctgcat tttcacgatc
     1621 tacggcatga agcaataagc cgcttcttcg aactgggtag cctgaatgta atggagattg
     1681 ctgcaatatc aggacatcgt tccatgaata tgctgaaacg gtatactcat cttcgtgcat
     1741 ggcaactggt cagtaagctt gatgcccgcc ggcggcagac acaaaaagtg gcagcatggt
     1801 ttgtgccgta tcctgcccat atcacgacca tcgatgaaga aaatgggcag aaagcgcatc
     1861 gtattgagat cggtgatttt gataaccttc acgtcactgc cacaacgaaa gaggaagcag
     1921 ttcaccgcgc cagtgaggtt ttgttgcgta cactggccat tgcagcacag aaaggcgaac
     1981 gtgtcccatc tcccggagcg ttacctgtta acgaccctga ctacattatg atttgccctc
     2041 tgaacccggg cagcaccccg ctgtaaacat cattcaaaac acatacagaa aggcgagttg
     2101 caacaactcg ccaaacacat tcgatctgca tgatcgaaat tattcgtttc accagaaaaa
     2161 acaccgaaga tacaataacc acatgagctg aaaagacttt ttcagtcgga ggaacaatga
     2221 gactgaatac gaccggtatt gcggcccgaa tgatgctctc gctggacaaa aaagcaatcg
     2281 gcgaaaaact catcaacggt cgtcagataa acccgacacc tctgcaggtc cgttaccccc
     2341 aaggggtaag agaagttctg gggataatga gcgaacaatt agccatatcc accgctgatt
     2401 taacccgaat cctgcttgag gatgctcttc ataatatgtt tatgcctgcg gataacacca
     2461 caggaaacat aataagcaga attgaataca tcatgctgtc gcatgatata aatgcgccat
     2521 tactggcaac attgctgtcg ccatggaata tccgctccac ggttatacag gacccggcca
     2581 gacttgcaga ctatctttca gctgacgctc tcgaacatct tgctgaatgt tttaatctca
     2641 atccggactg gctgaatggc catgaaaact atccgatagc gttgtccggt gaatggccag
     2701 atactgctga taatttcaga atgcttatta gtgatagcag caacactgaa gttattttct
     2761 ggcatagttt ccccttcgca ggaaatacga aaagagaata ttgcggggta atattgagac
     2821 agaaaaaaga aataaatggc tctgtaatat atccggcact atcattatca ccaacacttc
     2881 tgaacgatga aaaaagaaaa tggttaactg aatatacaac caggcagaat acaacaatgt
     2941 ctttgcgacg cgtcacgttg agacctggcc ttgccgggaa ccttattaca gggcaaatac
     3001 tacctgttag cttatttaat acgtctttat taccctggta aataccagat agtaccaaag
     3061 taaaaattac ggcacatccg tgtcatagcg ttgttttgtc taaattctga taaacaggaa
     3121 attaaatgat gaagaaaaat catattaccc gcaccatcat tgcatctgct gtattatttt
     3181 cctttaacgc tgcagcagcg acgtcatatt ttgaagcgcg taacgatgca atgggtggta
     3241 ccggggtagc atcttcccat tatggtgtgg cacctctggc aaacccggct ttactgacaa
     3301 agcacaacag caatgatgat ttcagccttc ttctgccgtc tgttggggca caagttgctg
     3361 atcctgatga tgtatcaaat aaagctgatg atgttaaaga tgactgggat ttgtttgaca
     3421 gtgctgtaga taaccagcat ggcgtacaac aggcagcggc aaatcttaaa catcgtctgc
     3481 aggaattccg caatatcaat gcagatgctc aggtaggtgt atcagctgta gcagccatgg
     3541 caaatgacac tctgccgttt gcccttatgg tcaagtctta cggaactgtc agcgtaaacg
     3601 gaaaagtaaa cgatgcagac ctggattatc tggacaaagt tgccaatgga acaattaccg
     3661 acgtcgataa gaatgccctg acctcacgcg cattcgggcg tgcagctgta attaccgacg
     3721 tcggaatttc atttgccaaa gaactggaaa cggccggaca aaaatggtct cttggcgtca
     3781 caccaaaata tcagcgtgtg gacctgttta actacaacgt aacggttcgt gattacgaca
     3841 aagacgattt tgacggtgac aagtaccaca acacgaaaaa cggattcaac gccgatattg
     3901 gtgcttatac agacctgaat gacaactgga ctgtcggtct tgttgcccag aacatcattc
     3961 cacgcagtat tgatactaaa gtcgtcaatg gctttaaaga aaccttcaaa gttcgtccgc
     4021 aggcaaccgc cggtgtatcc tggcataatg acctgtttac cactgctctt gacattgatc
     4081 tgactcctgc cagtggcttc acttctgaca gcaaacgtca gtttgcaagt gttggtgctg
     4141 aatttaacgc atggaaatgg gcgcaattgc gtgccggtta tcgtcagaac atggcatcaa
     4201 actccggaag cgccttcacc gctggcgttg gtatctctcc atttgacgtc gtacatatcg
     4261 atgtttccgg cctggtagga actgaccacg attacggtgc aatggcacaa cttcagttca
     4321 ctttctgatt actgtccccg cccccggcac taaacccggg ggtattttta tattcagttt
     4381 aaataacatg atgaaaccac gttcttctta ttcaaaaaca gcattcattc ttttattttc
     4441 tgttttcctg gtagcggcag tgacaaaagc aaaaagctcg cttcccgata tcaccctgga
     4501 acaggcaaaa gaaattaatg ctgataacac cgttattttc cttttccgcc atggagaacg
     4561 ttgcgaccgt tcagatatgc catgctattc ggataaaagc ggaatcacta ttaccggtac
     4621 agagaaagca cagcaggaag gtattaagtt cgcaacaatc ttcagtgaat atgatattta
     4681 ctccagcaat gcggtaagaa ccatacaaac tgcaaaattc ttctccggta aagaacccgt
     4741 cgttatggac agtttatcag actgcaataa tgatctttat aagacgcttg aatcaatcgc
     4801 aagagaatct cataaaagga acatagtaat aatgacacat aatcattgtt tatcatttct
     4861 ggccagagat aggctcggca agaaattcaa accggcgtac cttgatgctc tgatcatgca
     4921 ttacgatggt acccggctca tacttgacgg aaaatacaac aaagaggcat agaggatatt
     4981 tgaatttaca taaattctga tatactcagt aagatacaag agtgtacggg cacatgtgcc
     5041 tgtctgaatt gcaggttatc cggacgaggg ccgggaactg tttcagggac aagcgccggt
     5101 acgttcgatt cataaatcgt cgagtcatcc atcatggatc gattcggcgc ggagcgttaa
     5161 agattacgct catattcgtt ttatgtctga cgacacatca accagccagt atcaggaata
     5221 atcaccttca gtaaactatg aggctattta cctggccaca ctggcattta tttccggtaa
     5281 cgcaagtata gccggaaaca aacatcatcc tgtctgtacc ggagcaccgg tatatcccat
     5341 tctgatttaa ctgctcagga attattatga accgtattat acctgcgggg tgtcttgccg
     5401 ccctttttct ggctggctgc aactcccctt ctcagcatac tcctgttccg cgtaactacg
     5461 gtgctgcctc tgatgtggcg gttacccggc acactcagta cacattatgg gacaaaggtg
     5521 tattttccgg ccaacttccg gtttcaacgg ggtctcatct tctgacagct aacagcgatc
     5581 gcctttcctt tgactgggaa ggtgatgcaa ttgaattact gaacgaactg gccagagtca
     5641 gggggatgca gtttaactac aatggtgtcc gcctgccgtt accagtcaac cttcacgtcc
     5701 gggatatgac gttttccaat acgttgagac ttattgaagc ccagacagcc tggagagcaa
     5761 caatccatca atatccgggc ttgttacagg tcagcttcat gcagccggag aaccggaaaa
     5821 aatgaaagca ctcacctctc tccttttgcc agtaatactg cttactggtt gttcttcttc
     5881 ccctaccatc tcgccaggtg gtgtcaatac gccaccacct gacattaatg catggcttaa
     5941 accacgaagt gaaaagccgg atagtctgtc agaaacacgc tggaaaatgc tgacagacgc
     6001 aggtaaaacg cttggatttc gtggtggcaa ggctcagcgt tcatgggagc tgattcaggc
     6061 gctcaacgcc cgggaaagca cacttaatgc cttatacgac ttccggccac tgattagccc
     6121 ggaaggctgg ctaccaccgg ttattgatga agcacaggac gtagctcata tcacgccaga
     6181 tcaaatccgg acctcttccc gggtctggac catcattcgc cctgaacgct ttgtcagcaa
     6241 tccaccgggc tggcgtgact ggcttctccg ggggctttca acaacagcca ctccgggtac
     6301 agaaggcagc gtcgttccag aagacagcgt gcaacgaaaa gtgtgggaaa ccgcattacg
     6361 gcagggatgg caggaaggaa gacaaaacgc cgacctgacg cttgaagcca accagaaaac
     6421 cctcacccgt gattaccggg gcatgatgct gtactcactt ctctggcgtc agggcatgat
     6481 cacccgtccg gatgtttctg atcaaatgca aaccgttaca ggtgacggga aaaaactggt
     6541 cactggagat cgtgttcgtc gccttaaaaa tcatgcggaa ttcaatcttc agaaatctca
     6601 ctggcgtccg cttataggca ctgaaggagg ctcccgatga atattcctga tgagtttggt
     6661 ctttttccgt ttcagcgttt cactgcagat gaactacggc atttctttgt ctggtgtgcc
     6721 gcacataaag tcagtgatgt ggatctgacc ggcgggtcac ctgtatctgt cagtcgcttt
     6781 ggccgacggg tgcgttgttc cagtgcaacg ttaccaacca cactcatgtc ctccctgatt
     6841 gatgaactgt tcggtcggga agtcattccc cgcgttctgg caggcaatcc ggttgacagg
     6901 acgatacaga ttaacgggga tgcatccggg cgctatggtc ttaaacgcgg agaacgcatc
     6961 cggttacgct ctcacctcat tcagggcaca tctggtgcgg aggagaaagc aatctcaata
     7021 accatgcgtg tcatcccgac agaaataccg gacattctct ccatgaacat tgaaccagac
     7081 ctgcttgaag caatggtctg caaaagcggt ctgggatttg tatgtggtga aaccgggtca
     7141 ggaaaatcaa cgctgtgttc agcactgtac cgttacatta tggacaattt ccccgacgcc
     7201 aaaatcgtga catatgaaga cccggtggaa tatatcctgg gaaatgaaaa cgatctgctc
     7261 ccaccgcatc aggcagaaat cgggcgtgat gtggtcagtt ttgctgccgg actgcgttct
     7321 gctgtacgac gtaatcccga aattatcggt gttggtgaga tcagggataa cgaaaccgcc
     7381 gacgcagcgg tacaggcagg caacaccggg cattactgcc tgagcaccat gcatacgaaa
     7441 tctcctggtg aaacgctggc acgtcttctt gggctgtttc cccctgtcat cagggattcg
     7501 atggcctggg ctgtcctgtc actgttgcag ttcattctgg ttcaggttct ggtcagaaca
     7561 aatgacggtg gtcgtaaagc cgtccgggaa tatatcgtca ttaacgatga gcttcgcgac
     7621 aatctttccg gaatgccgca tgcagagtgg gggcatcaca ttgatgccat aatccggcag
     7681 gagaagcgcc gtatccggga ccagattctg gaaatgtata tccggaatga agttgaccga
     7741 agagaagcga ttctttttat cccgccaggg gagttacggt catgaaaaga gaaggtttct
     7801 ggcgatatgc aacaccgcca gtcaacatcc agggcgtacc tctgccgttc accatgattt
     7861 atctgtttat atgccccttt ccctccaaaa caaccttctg gatttgcacc ggcatcattt
     7921 tatttttcgt tattctcgac cgttcaggct ggactgtcag gactatctgt acacgcattt
     7981 tcagcattct gagaggggcc atcgcctccg gtcgtccatg gtggtatcgc caccatacgg
     8041 aatcaccacg agactggact ggcctcgatt aaattctgga gtctttatgt caggtaaaaa
     8101 aactatcgtt actctgctcc gggtatcgct actggcatgc ccgttactgt tcacgacacc
     8161 atcgtttgca atgatcgaca cgccatccgt gaaggtcggc ttttctcctg aaggaagcgc
     8221 atccgctctg gtgcttgaca caatcaacag tgcggaaagt tccatcagga tgatggcata
     8281 ttccttcacc gacccggatg taatgcatgc gctggcaaaa gcgaaaaaac gcggagtgga
     8341 cgtccgtatt gttgttgatg acaaggggaa tacgaaccgg gcaagtcagg aagcgatgaa
     8401 atatatcaac ctgctggaca ttcctctccg gactgtagat gccttcccca tccatcacga
     8461 caaagtcatc attgttgacg gaaacacggt tgaaactggc tcctataact tctctcgtgc
     8521 tgctgcacgc aagaattcag agaatgtcgt tgtgctcaag aatatgccgg atgtagccgc
     8581 acaatatctt gaacactggc aggatcgctg gaataagggt acagactgga aaccctgaca
     8641 aacatttctc ccccgatttt ctatcccccc agccaccccc ggcttccggc ggtggttttt
     8701 ttccgtcttt tttcaggaga agcactcatg ccgtctgtat cccgctaccg cacctggctt
     8761 gccgtgcctg cagatgaaat agaagatctc aaaaaagcac atcccccgat gaacgggcac
     8821 acgcccgtcc tctgggataa agagcacaaa ctctggtttg cccgcccggg ggcggacctg
     8881 tccatgctgg accgctggct gccccgaccg caggaagtct ccatgaacgg cagtgatccg
     8941 gtcaccgagt ttgcccaggt actggaaaat gccggactgg ttctgaaaga actgccggcc
     9001 atggacggta aaatccaccg ggttccgacc accgatgaca aaaacgggca aaaaagtggc
     9061 gcatatcgcg gcttcctgga cggcagacct gccggctggt acagggatta tcgcagcgcg
     9121 gatgattcgc cggtcaactg gaccttctcc ggcggagagc agacagatcc ccgcgcaagg
     9181 cttcacctga aagcgcattc gctgcaacgc agggaggacg ctgagcggga actgaaagca
     9241 cagtacaacc gacaggctgc ttatgcccgc cgctatgtca acaaatggcc gcaggccacg
     9301 gcacatgaat acctgacccg gaaaggcatt caggctgcgc cgggggtgcg aataaacgac
     9361 aaaaatgagc tcgttattcc tttccgcaac cgtaacggcg caatacgctc ctaccagcgc
     9421 attccggtca ccggcgggaa ggatgcccgc atcctgaaag attccgagaa aaccggtaac
     9481 tggtttgcgc tgggtacacc gcgcaacggc cagccggtgc tgtttgccga gggctatgcc
     9541 actgccgcct ccctgcacga agccaccggc cttccggtgc tgatgacagt ggatgcgggc
     9601 aacatgattg ccgtggccga agatgcccgg caaaaatgga agcaaagccc ctttatcttc
     9661 tgtgccgaca acgatcatgc acgccaggtt aacaaaggta tcgtcagtgc aacaaaagcc
     9721 gcagaactga caggaggatc ggtcatcttt cctgcgttca ccgatgccga aaaagcgcag
     9781 ggactgaccg atttcaatga cctggacgcc agccggggac gggccgcttt tcagcatgtc
     9841 atcaacgccc agttagaaca tatcggggtt tccaccccca acagtaacac cccggaaatc
     9901 cgggaggcac tggtgatcgg caacctcgtt tttacaccgg tccacactga ggaaaaaacc
     9961 atgactccga cagaatatcc tgaaacatca cccgatacag ggcacagcca tgaccaggga
    10021 ccttctctgc cgtccgccac gcagcaggaa caaccaactg cttccagcag taacattgct
    10081 gatgaaacac tgtcatttac cagtcacgcc actgaaaata acggcaaaga tgagcggcac
    10141 cctgataacg ttcaggcgca aacggaaagc tctgtagttc aggactcccc tgcagaatca
    10201 ccggcatccc cggaaagtac aacggcgtct gcccctgatg aacccgctca tccggcggaa
    10261 cctccggaaa aagttgtctc tgtggccaca gataaagact ggcgtgaatt tgaagccgaa
    10321 ctgtcacaac cggaaaaaaa cgagttacaa caggagtccg gcacttcacc agaaataccg
    10381 gcccctgctg ctgcccccgc cgcccctgaa gacagcccct catctgaaac catgaatgaa
    10441 tcagccgaac catcaccttc ccctgatgca gtcaaagagg aaatgaccgt gatgacggag
    10501 cctgatgaca aacaaagcac cggaatatcc ccggatactc cccgggaaga ggcggtcagc
    10561 catgtaactg aagacattcc ccttcagcca gagccgttcc ttcctgagtc agatggccct
    10621 gaagaatacg aagactacag cgcatatcag gagctgatga acaatgaaaa tcctgagcat
    10681 ctgcaggaaa acagtgatat gccgtcgcaa ccggacacag gccccgttca ggcagatgag
    10741 tcagaacttc aggccacaac agccactata gaaccggctg tccgggatga acccactccg
    10801 cagcaacctg ttcagagcac atcaccttct gataatactt catcgtttct cgacaaagcg
    10861 cgtggccttt ttacgcgtaa aaaaacggat tcacaggcgc atgagaacag tgatcccacg
    10921 ccctcagcgg aaaccacgac gactccaccg atacctgaca gtattgtcta cgccccggaa
    10981 cgttctgatg caccgatatc gctcaacatg gacgaaattc ttaaagagct tgactgggaa
    11041 aaacgagcag acagaaccgt cctgtacaaa ctggatggca aacctgcctt cattgaccgg
    11101 gtaaaccggc tggaaatggt caacggtgcc agcaatgacg atcgcagcgt tctggccgca
    11161 cttgctgtgg ccaccaaatt ttatgggggc gtcatagagc tgacgggcag tgatgccttt
    11221 aagcaaaaag caatgcagtt gattatcgag cacaacatcg atgtccggat gaaattgcca
    11281 gatcaacggg ctgaactaga aaaattacgc aaggaaatgg ctgtcagtaa ggatgcagtt
    11341 gtcacccatc agccaacacc ggaacttaac cggaataccc cagaacaacc ggctgtccct
    11401 gaccccgttc aggaaaaaga agccacacaa tccccggcgt ccccagtagc ccctgaagca
    11461 tcaaccgtca gcaccgcctc tgcaccatca ccggctgaac caggcaaaac ggcagatgtg
    11521 gcaccagacg aagaaccacc aggaaaactt cgcccgggag agagcgtgac tgccgtactg
    11581 cacaattttg gtcgtgctga atatgcaccc ggcaaaggag agagtttctt tgttgagctt
    11641 aaaaaccgca gtggcagcaa actctactgg ggggaacaac tggaaagtct ggtcaaaaac
    11701 catcagaaag gtgacgtggt gacactgaca cttcagaacc gcgaacaatt catcctcccg
    11761 ggagagcaaa aagcacgttt ccggaataag tggtccatgg agtcagttac caacgggatc
    11821 tcggtatcac atgataatcc ggataaaggt cagcgtattc aggcgattcc ggtcgaaacc
    11881 tttatgaaag tggctgcgca aatcagccag gggtggcccg aagagatgaa agcgttacgt
    11941 atgccagaaa acgtcggctc acatctgttt atcggtgaag accgacatcc ggtctccgcg
    12001 ccacagaacg caaatcaggt aacagaaata accagcgcag cccctgacaa gctgacacct
    12061 gttctgggat cagtggataa ggatacgcgt gaactcaacc tgctacttgt ccagagtgcc
    12121 gatgaacatc tgcagggtgt ggtccggctg aacggcacat tgtatccggc actggccacc
    12181 ccatcagccg acaacagtca gctggttatc aatgcgctga ccgacaaagg tttacgcttt
    12241 gcaggttatg gtgaagcggt aaaccacgat gctgacagca cgaatcgccc tgcaccggag
    12301 cttatgcagt ttcatctgaa aacccgggaa gaaccgcttt ttgctgctgt ctacacgccg
    12361 gaaaaacaac ctgatgcgtt gtaccggaac ctgggcttcg agcagtcatg gcagcagtgg
    12421 tccaacagcc aaaagccgga ggacaggcag gaaaaaactc tgcatcagga tctctcccac
    12481 agccccggcc gatgagccgg taaggaagcc atatgaacat gcataagtct ctggttgccg
    12541 tgctcggcct tatcctgatt tcatccacgg caagtgctgc aacctgtcgt gcgggtagcg
    12601 cggcacaggc cggtagtaat acaggatatg agcgagcacg tgcagctgcg gatgcatggt
    12661 cacaacgtga aaacgatgtt tcctcatccc tgcagtcctg tctgtcacga atcaaaaaaa
    12721 tcagcataaa cctcccccag ttccccagcc ttgacgacat tctgtcgcag ctggaaaacc
    12781 aggtctgcga cgcggtcgtg gacaaggtga atgaaaagct gccggggaat attgatccct
    12841 ggaaggacta caacctatga ccgaaaatac cgagccatcc tcgccatcac atccggcacc
    12901 tgacccggaa atactgaaga gcacaatcag ggctatgaag cagtcagaac agcgggcaaa
    12961 taatgtccct gcgctgataa aagcgctttt atgtacgggc acatgtcttc ttatcagtat
    13021 tactggtaac gccattcagt actggcactc aacgaatgtg gagcgcgaat atttcgctac
    13081 agacaatggt cgtctggtca ggctggcacc aacaagtcag ccagcatgga gtcagaatga
    13141 tgcaatggcc tttggcagcc aggcactggc gaccgcattc aacctggatt ttgtgcacta
    13201 tcgttcacag atctcctctc tgtctccgcg tttttctgac gaaggttttg ccggatatgt
    13261 gaatgcatta caggcatcta acattctgga gacgatcaag aaagagaaga tgaatctgac
    13321 cgccacgaca ggcgctggcg ttctggttcg acagggacaa atgtccgacg gcgtatggtt
    13381 ctggacattt cagtatcctg tccgtatgcg tctggtcggc cagacaacca gcaagccaga
    13441 acagtctttt gttttcgaaa tcaccatcca gcgtgttgac cccagactga aaccatccgg
    13501 tatggaaatc cggcagatga tctcccgtaa cgccggaccg aactcctgag ataagtaata
    13561 tgcaatcacg ttatttactg agcacactgc tcctggtgtg cagcgccgcc acgtctgcag
    13621 ataacgcagg atggcagaac gcacgcacac cacaaacgac aaattctgcc agcgatcacg
    13681 ttcagaacgc ctcccaaaac accggaggtg ttccggcaac actggtaaaa ggtgagcttc
    13741 ctgcccccgg gcaggccagc ccgctggtac aggatgcggc aagacttgat tctgagctct
    13801 ccgccgatga gattcgctca ctacgcagtc tcatggcgga taacgagcgg gccatcaatg
    13861 cgcccatcac cagtgttgta ccccgtatca gctctctgac agttaatctg tcccccggtg
    13921 ccagccttcc tcttgttcgc acagccatga acaacctgag cgtagtgaca tttaccgata
    13981 tcaacggttc accatggcca caatccgatc ctccttataa cgcggcacca aaactctttg
    14041 atgtgcagta caacgagaac atggtgacaa tcacaccatt gcggccatgg gcatccggaa
    14101 acatttctgt ctacctgaaa ggtctgtcgg taccggtcat cctgaacgta accagtggcg
    14161 aaacagatac gccgtcatcc agtcaggaaa tggacagtcg tctggatctg cgaataccac
    14221 ggcagggacc aaccagcccg gttgtctcca tcccgacgga taaaatcgcc ctgcatgatg
    14281 caacattaca ggcatttctg gatggtattc ctccccggga cccttcagtt aagaggctga
    14341 aattcacagg taatgtcccg gatacaacga tctggcaaca cggcgatgac ctactggttc
    14401 gttcccgggc gatgttgcgc gatgaatttg aacagacctt gtcctctgcc gacggaactc
    14461 atctctggaa acttcccgtc accccactac tgactttctc tgtgaatggc cagtccgttc
    14521 acgtgacacc agaactggag taatcatgag tgctgaacag gatgccggta aatccggaaa
    14581 gaaactggct gccctgcttg gactgggcag catcatctta tttggcgtcg gctatatcgc
    14641 gttttccaaa atatcaggga gtaacagcga tatgcagtca gccgtgaaca ttaacagtgc
    14701 agcaagcggc ggaacgcgca gcgttaccga aacgcctcac tatcgtgaac tgttaagagc
    14761 tgataatgaa cgaggggccg cagctgcagc ccgcaataat cagacgttca ttgccagtct
    14821 tccacagggg cttgatattc cggacactcc ggaaaagcaa caacagcctg cagcaaaacc
    14881 ggaaaactat gctcatcgcc aggcttccgg cacaccacag gaggatcgag cagccagtga
    14941 aaaacgtatg gagcggctgc aaaagctgat tgtaagaata aaagatcagc atcctgccgg
    15001 tagtacacca accatagcaa cggctatgtg gaataaatct ccggcagaaa caacagggca
    15061 gaatggtacg cagcaattcg cattacagaa tgccagcctg tccacgccgg tagcagaaaa
    15121 agggatacaa ctgatacctg ctctgacccg tataccagca tatattgata cggcagtgga
    15181 ttctgataac ccgtcatcca aggttatcgc aaccattccc gctggcccct gggcaggggc
    15241 aacgctgttc agtccgggag tcaaactggt gggtaacggc gtggagatcc atttcgaccg
    15301 gatgagctgg aacggcatgg accttaaggt caatgcatat gctcaacggg aagacaacct
    15361 gatgtcatcc gtggccagca atgtgaatac acgctggttt aaacatatca ttctgccgtc
    15421 agtgttagga ggtgtgggca gtattggcac actttataaa gatgccaaca cgcaggttat
    15481 tcagggtaac tatggcacgg ttaccggacg cgtgggaatg cccagtggtg aagctgttgc
    15541 gggcgttatt gccggtggta tggcggaaag aggaagtcag atattaaccc gccaggcaga
    15601 aagtgagccc tataaacagg tagaagtgta tcagcatgaa gtcgtatcaa ttctgtttgt
    15661 tgacccggta atgacaaacg acgccaggtc atcttccctt tccagtggca tttccccttc
    15721 agttaatcgt acatcacaga cggaacaacg ctcgcaggca cgaatgcaga cagccatgga
    15781 gcaaagaaaa gctgtgatgc aacgtcggta tgatgaacaa ccggagacac cataatgaaa
    15841 cctgaaacag aaatagatga aaccggcagc ttcggtgaac ccgaagagaa acccgcccct
    15901 ttctggaaac gttccgtctg ggggatatct gtagcaacat gggggctatg tgcagtcgta
    15961 ttactcgcag ccatatggta tctgttcctg agagcaccat cggaaacagg catgcctccg
    16021 tttaatgatg cggatgctgg cgtacagacc tggcaaacca cacaagaatc atcaccttcc
    16081 gttcagtcca ctgagaccat gactgtccgg acaggagaag atatgagcca gcttgcccgg
    16141 gatgtaaaaa cagagctgga taaccgcgat gaaaaaattc aggcaacact gaatatgctt
    16201 cacgacagca ttaacaagct gggcgaagct ataaaaaagg atgaagaata cgcacaggaa
    16261 acgcgccgtc agctggatga tatccggagc cacctgaacg gaatgatgac acagaaaagc
    16321 gtcaccgaat cctcttccac accgcatcca gcaaagaaaa aaacgtcgtc tgtacttaac
    16381 ggaatgaaaa tcatgtcgat ggaaactggc atggcctgga ttcgctggca gggtagtacc
    16441 tgggctgtca gagaaggtca gactctgggc aatgtggtga tccagcggat tgatccgacg
    16501 acccggacca tcatcaccag tgcagggacg ttacgctgat gaacatggat gccctgaccg
    16561 ccattgagaa ctttgccagc agtattttca gtgccggaat ggatttcctg tttacctggg
    16621 gagaattcat cggggtgatc agcatgatta ccctgtttgc ccgcgcccgt tcagccggtc
    16681 cggtaaaaat gtctcccgga aaatttattg cggggatgtt aaccagctgc atgctcgtat
    16741 cactcccggc aatgattaac gccggaggcg tacaaatggg attcagggct gactcatttg
    16801 gcccgattgc atatgtacaa cctcagacgt ttggcgctgc tgctggcgca gcaaatgcag
    16861 ttctgtcgct ggccaagctg gccggtgttg gatttgtaat gaacgggata tccatctggc
    16921 gcaaggccgg actcgatgga cataccgcac tgagtgccag tgaatccgtc agtaagggaa
    16981 acgtcaaatt tattgccggc gtcctgctgg tctttatcga tcgtgtcctc aatgccctgc
    17041 tggcaagcat tggaatcgtc ttctgatttt tttcaccttc atataaacca ctgatatatc
    17101 catttaagga gaaactatgt cacgtattcg tgtcgtagcg gcccgtctgt acgccgcatt
    17161 tatccacgca caactgtttg taatgtcatg ccggagcaaa ctgactaagt tcctgctgct
    17221 cattcccatg atgttgctgc ccaaagccgt tcttgccgat ggagatcttg ccgatatggt
    17281 tcgcaatgtc gaacaaggtg ccaaaacagc acagtcatca tcactgacta ttgcgcagtt
    17341 catcggcgta attctgtttc tcgggggcct gattggcctg aaaaaagttg ggaaacaagg
    17401 ggggatggga ctggctggtt gcattgtatc tatcgtcatt ggggcgctac ttgtcgccgg
    17461 acctgaaatg atgagtcgca gccagaaaca gctggggatt tccacaattt ccattggcta
    17521 atcttgtcca gggcactggc atttatccgg tgcccttcct gttcggcaat acaaggaggc
    17581 acatatgtct gcctggagcc cggagcacat aacgttacga cgaacagaaa acggcaatgt
    17641 gattgcccac gacagtcggg acgatcagga attcattttt gcagaatgct gttccgatga
    17701 aatcgcaaac atcattctgg aagccgtgaa acgttacacc agtacggagc ctgctcatgc
    17761 aacacagcat taaagacttg tggttgtatc cctttccaga aattgatgtg gttcatacac
    17821 aggagccact gttaccggaa ccggaattaa caacgcccgg tcgatgtatc tgctgccggc
    17881 aaaacgtcag gcaccgtttc agactggatg actcatggcc actcagacag cttaccgaca
    17941 caatcagtga cacacgagtt cgtcttaata aggccacaga acatctggat aagttaaaaa
    18001 agcgtggtga accagtcgca accggcgaaa aggaaaagta caacacggca gtaaaagccg
    18061 ctgaacgggc gcttgagcag gcccgtctgt ctgccagacg tctttcactc cgccatgtac
    18121 agaaagctga aatcaccagt acagagtcgt tgtcagagaa agagcaggaa ctgtttcatg
    18181 aagacggccc gccttacagc ttatgtgctt tctgccacgc ctggcattca ctgaatggtt
    18241 atgccgcagc acagggcgtt atggtgtggc tacccgacct tcatccatcc acggtcgtgg
    18301 cccttaaccg tcgttcatta caggaagttt tcagtaatga taagttccgg gtccggcggg
    18361 gacgggaagc gctctctgca ctgatgcaaa acagactggc ggttgaggat aaattccgca
    18421 gtttccgccc ggccgatttc gctgacgttt ttcgtcgcta tccaccttcc ggtcgttcgc
    18481 ccctgcgtga aaaaatgaac gggattgccc tgattctgac accagattca ttcatcaaaa
    18541 aagaatacgt tgattgattt ttcttcatgc cactccagcg cggggtggca ttaaaaaaat
    18601 caaactgcac aaacctccct cctgtaagtc cggagttccc tcacctatga acataaaccg
    18661 tttaatcttc actatcgaag actgtctgaa cacgctatcc cgtttctctg tcgcaagcag
    18721 ttttgttgag tattgcgatt tacgtactgt aattggtctg gacagacagg atcgtgaacg
    18781 tcgcccatgg ctgaattcgc cctatattgc agccacaaag cgtggagaat atctctctgt
    18841 ctttgaagtc agcggtgctt tcagggaaat ggatgaagca tccgaccaga ctggcccagg
    18901 ttcactggaa tcgctgatca ccagcatgag tgacagcctg aatacggcat ataaaaacag
    18961 cggtcataaa atcagcttcg tttttgagcg tgatcctgac atggggaaag aagaaatcga
    19021 agacatggtc gccccacaaa agcgctcact ggctaataca ggtatccagt tacaggatgt
    19081 ggtcgatgag aaagtcacaa cactgtcccc atggctggtc cgggagcgtt gctggctggc
    19141 gatctggagt ggacccgacc ttatcagcaa cagcgatcgc accgcccatg atgagcttgt
    19201 cagaagactg gctgaacggg taccgaaagc acgctttgcc cagtccccct ggcagtggac
    19261 cttatctgca ctgaaaatcc gccacgaagc ttttcttgat aatgtggaac aggcactgag
    19321 acattcatct gatggcctga ttctccggct actggatata cacgaggtcg gacgtgaaat
    19381 acgtcgtcag acagaacggc acagcacacc gcgtaactgg caaccccacc tgcctgaaga
    19441 tgcccagcca gcgggatatc gctggactga cgatgaatcc gtactacatg ctccttcatt
    19501 gcatttgcag ttattcaaca ctcaggtgac cacacagggc aacctggttc aggcgggtgg
    19561 actctggcac ggaatggtat cgattacttt acctccgcaa aatctgcaga cgtttaacga
    19621 actggtccgt gccgtccccc gcgcagtccc ctggcgcatc agaatggatc tgatgcccgg
    19681 cgggatgaag gctcttaatc tgaagaaaac gcttctgact tacagcagct tcatttctgc
    19741 cgttcgtcca atgtatgaat ccgttatgac cctggcggca acggatgaaa aagaacctgt
    19801 ctgcataatg accatcatgg cctccacgtg gggaaaaaca cgtgaaatat gcacacgaaa
    19861 ccaggccatt ctgaaatcgg ccattgaagg ctggggcgta tgtggcacca cgacaacatt
    19921 tggcgatccc cgacgcgcct gggtgaatac cattctcgcg gcatccggag gctccggccc
    19981 ggttcccctg tatcccccgt tgtcacatgc catctcgctt ttcccgctta acagggcagg
    20041 ctctgtctgg cgaggcaaag ggaatctgat gttacataca gaagacggtt ccgcatttga
    20101 ggttggtctg gcgtcatccc aacaaaataa acatacagaa ctggcaccag gcgatccggg
    20161 actgggtaaa tcagttctga ttaatacgtt gtcggaaata cagatatcat ctgctcagaa
    20221 aaacctgccc ttcattgcct atatcgataa gggatacagt gctcagggac tggttcagct
    20281 tattcgcgac agtcttcctc cggaaagaaa agacgaagct gtcggtatta ttctgagcaa
    20341 tgatccggaa tacacccgta acctttttga cgtgatgtac ggcgctaaaa agcctattac
    20401 gccggaaaag aatttcatgt catctgtgct gtgtgctctc tgtgtcgata ccggtaccgg
    20461 tcaaccctgt aaccccggag acacccgaca aattatcaac cagcttatcg agctggcttt
    20521 taaagagtac ggtgaaaata atccgcgact ttatcgcgcc tcaacggaag aactggtgga
    20581 ttcagcactg caggactcgg gactgtatga aaaacatgat gcaacctggt gggccagatc
    20641 aacctggttc gaagtccggg atatgctcca caatgccggg tatatcatgg cggctcagcg
    20701 ggcacattat caggcaatgc cccagcttcc cgaagtgtcc tcgatgctgg gccataccag
    20761 tctcagggat gttttcggta ctgttcagcg tgatggttca aacgaacttc tgctggacta
    20821 catccgccgc gctctcgagc agggacataa cgactacccc atgatatccg ggtatacccg
    20881 gtttatgatc aacccggaaa cccgggtcat cgctgtcgat ctgaataatg ttgccggtga
    20941 caaaacgcct gcaggcaggc ttaaaaccgg catcatgtac ctgcttgcag gtcagatcgc
    21001 cggtggtgac ttcaccctgc cacaatacag ggatgaagtg ttaaagcagc ttccacggga
    21061 ataccatgaa atcgctctta aacgtattaa ccaacttgat caggaggtta agacaaaagt
    21121 ttatgatgaa ctgcataacg cccggggcat tgacttcatc tgggaaaatc tggataccca
    21181 ggagcgtgag caacgcaagt ttgctatccg cacggttctg agtacgcagt atctgaggga
    21241 ctacccggaa agcgttctta agtcagcaaa tactctctgg ttactgcgct acaaacccga
    21301 agatatcccc gtactgaggg ataacttcaa cgttcctgaa tttatgctga aacgcttcct
    21361 gaaaatgccg gaaggcccgg caccagatgg tagtggtgtt cctgttctgg gagtattcag
    21421 ggtaaaaagt ggcacgcttg cgagaatact caaatttacc gtcggtccac tggagctatg
    21481 ggcgctgaac tcatcgccaa aagacagtgc cctgcgtaaa acactgacaa ataagttagg
    21541 ttcagttcgg gcacgtaaga ttctggccga aaacttcccc agaggttctg caacgtcatt
    21601 aattgagcac cgggcgggcc aacataacag tgacaatgtt attgaagaac tggcctcgga
    21661 gctcatccga aaacagggat acaatctctg atgtgtacaa ttcatatcac cccacgctgc
    21721 cggagacctg ccagagggca ggtatttctt aaaaaacttg aggtaaaagg tgacagtttc
    21781 tcattcactc tgccgtcacg tgtcttcagg ttacatccgg cccccaatcc cagagtgata
    21841 tacgcctcgg atgccagaac acagggaaag gtcacaggca tgcggctgtt gttgccgtcc
    21901 agcacagatg agctgataac aacggcaata tggcaggttt ctgtcccgga tgaagcacca
    21961 ttcgacgttc agcatgtcat ccagtggata ctcccttcac cgctggcttc gctgatgtcc
    22021 gatgatacct ggttatggcc atccgttccc ggaacgccgg cactaagtgc cggtgactgg
    22081 cctgttatgg atactgaccc ttccacctta cggcgcgtca gacgtcgttc tttcagcatc
    22141 atcgataacc ggaacaggaa aggttatatc accaggcgat atcagtgttt cccactggcc
    22201 ccactacctg aagacagaaa atacgacagc cttcctgacc ttgtgctcca tcccgagcag
    22261 gagacaataa aatgcaaaga aaaacattac tcgcagccct tatagccacc ctgagtggaa
    22321 cggcatgcca ggctcatgct tattcagtca ccgtcgtcgc cagccggcct gttgaagagc
    22381 aggtcatccc cagaatggaa gccatcaaag atgtccttgg caacatactg agtacccaga
    22441 cagccacggg taccgctatc aatcagaact ctgaaaagct ggcaagtgtc attgcccaga
    22501 acggacaggc gacacgtcag cagatgattt tcagtaatga aactcagcgc ctcgaggagg
    22561 cacggaaaag cttcaccgtg ccagactcca tctgcagtga atccgcatca gggattgcaa
    22621 cagaaagcaa aagtgcctca gcatccgcag cctcaaaact cagtaaaggt ggcggtgtga
    22681 gcaaccgtag catccgcgac cgtctggcca gcgcagcaaa ctctcccgtg cgggaagctt
    22741 atgatggcgc ggccatacac gccagttact gtacagaagc agaatatgca cgttttggag
    22801 gaaccgccgt ttgtccttct gtgggcgaaa taccaggtgg agacagccag gtacgctcga
    22861 tttaccacgg cgcaggtaca gcggacacac ctgcggcact gacatgggat cagaagcaaa
    22921 tagatgcagc gactgcttac atgaaaaaca cttcccgccc ctctgccggt cgtgctctgg
    22981 gtaaaggaga agtgaatacc cagagtgggc gaacctacgt cggactccag aacgaataca
    23041 acggcatcat tgattctgcg tcgaatcccc aactgacgct gattgctgac agcacgccta
    23101 atgaaagcac acgtaaagca ctggctgaaa cactgcagtc agactcagcg gccgcttact
    23161 tcgaccaggt ggcttcacca gaagcaaaag cacggggata tatgagtacc cgtgagtttg
    23221 aagcttttga ggctggccgg cgctacgcca acacagcata cctggtggat ttacaggaaa
    23281 tgcaggggga taacctgctc cgtgaactgg taagaatcac tgcgcaaatg aactggcagc
    23341 tgaacgatct gaaagaacag atccgccagg gtaatgttat ctctggccag caactggctc
    23401 tgactgcccg ccagtattat gaaaaacaac tcggtagcct tgagaaaaca atcaatcagg
    23461 caaacgcacg ttgaaagctg acgtcagaac aggactgaaa aaatgaataa gttaccggaa
    23521 aattcagacc agacgaaaaa cgtcaaaaaa tcaccatcca taacccgcat agccacccgg
    23581 gggattatta acatttttgt tcctctgatg gagactcgcc tgataacgaa ttctgtacag
    23641 catatgctgg aacaacaaaa acgcaggctg gacagatttc gtcaggtaaa taataaaaag
    23701 gcgcaactcc acctcacgtg ggaagaagct ctgcaggcca gccatatgtc agtagacgat
    23761 ctggatcgtc gtttccgccg gcgcagaaca gtatggcgat tctgttgctg gagtctgctg
    23821 gccatcgctt tgtttctcag tggtatgctc tttgccgcct cttcattacc tcttacgact
    23881 ctggtacgcg ccatcagcac tcttgtgttg atactgtcag gcgtcgctct ttgtgcttcc
    23941 agagcactga ttgttacata ccgcctctgg cagttgcatg aacgaaaagt cagcgaaccc
    24001 gagcagggga ctttccgtga ttttctgaac gaccgcaatg gctggcgcaa tgccacatta
    24061 attgccgtaa ccagtaaaca atactgatca cgtccagatg ccagtaccac tggcatgtcg
    24121 ccatccccct taattaaatc ctgtgtggcc tttgcccata cgcaattcta ttccggagtt
    24181 actgtgaaaa ttttacttcg cgcactctgt gcaggccttg ctatatcctc actgccggca
    24241 atggcttcag tgacctatca ggatattgtg tctgccgcaa ccaatcctga cgatctgtcc
    24301 cgtcaggcgc tggtcacgat attcggtgat gtagtcacca atccactgtc aaccagcgcc
    24361 cccactctga tcggaagcat gttcggggcg ttcaacagta tcatcgccgt acttgccgtt
    24421 gtctggttta tgttcattgg tatccgtcat gttgtgcgtt cggggcatca gggacaggta
    24481 ttcagcaccg gacgcgatat cgtcggcaca ctgagtgtcg ttgcaggttt tctgatgatc
    24541 gttccaaccg gaaatggctg gagtatcgct cagctcatta tgttatgggg cgcatcgata
    24601 atgggggttg ggtccgccaa tgtgatggtg caacttgccg ctgataatat cgcaaatggt
    24661 tattcaatga ctgtacagcc agttcaggca tccacacgaa cagcagcgcg tggcattttt
    24721 gaaatggaac tgtgtaagta tgcggttaac gcaggactaa atgattttaa tcagacggtc
    24781 aaatcctcga cttcactgat gacagaatca gctaaaaccg ccagtggtaa ttacactgtc
    24841 acggtatcca atggcagcgg tatctgtggt tcagccagcc tgtcagtaga aggaaacgga
    24901 actacagatc aatcgacgat tggaaaattc tttaatccct tcagtaaaaa cgaatacagc
    24961 ggtgttatct cagcacaacg tgcagccatg gataatatga ttagcgacat ggacaacgct
    25021 gccagtgagt ttgtgacaac atttctggaa aaaagaaatt ctggcaacgg aacattgccg
    25081 gatatcgaga cacggattca gagagcagcc gacgagtatg aacgcgctgt acaaaaatcg
    25141 ctacctattg ataacggaga acaatccagg aaagaagctc tcaagtctta tctcacaacc
    25201 tatggatggg tgacgcttgg cgcgtggtat cagacctttg ccactgctaa ccagcgtctg
    25261 gcagagctgg ctgacagagc tccagctgta acttcaatgt catcacttgg ggaagttggc
    25321 gatactgatt tatttagtgc tgttatgggg gcatacaaga ctcaattaca gaactccaca
    25381 tatactccaa cgataggaac gataacaact caagatgaaa gtaatgcagc taatagcaca
    25441 gacccacaaa gtgtggtcat gaaaagtata gcgccaacca tattaaaatg gacaaaccaa
    25501 gctgctactg aatggtcagg aacaggaaca accagcaacc aagttaaccc attaattaag
    25561 atgaaaaaca tcggcgatta tacattgggt acaacagaaa ttctctggac aggatataca
    25621 actgtcagag ttctcgcgac aatgggtgat aactctattt tcggaaaggc tgttaatttg
    25681 ttctctggat tacctaaagg atttgttgct ctgctggatg ctgcagctcc cccaatatat
    25741 ttcttgttgt tcctgttgtt ttgtgcgggt ttttccttat caatttacct accatttata
    25801 ccattcattt tctggatgac aggcattggg aactggatag taagcgtatt aataggatgc
    25861 accgccgggc ctctgtgggc agcaacacat ctaggaacat cagaggacag aggaagtcga
    25921 gcagcttacg gatatatata cttaattgac agcatgataa gaccacctct catggtattc
    25981 ggtttctttt ttgcctctgt cgccatcatt gccgtcggca caatattaaa ctcactgttt
    26041 ggcgcagcac tagttaacgt acaggtaaaa tcattaacag ggatattcag ccttgccggg
    26101 ttcctgctaa tttacgcccg tatgtgtaca acgtctgtag ctgcaatatt tgctttacaa
    26161 gcatatcttc ctgaccatgt tatcaatttc ttgggtggta gagatggtgt aaatactctt
    26221 ggcaacctca cctcatcagt aaaagatatt tttgcaggtt ctaatagaaa tattagacac
    26281 agccctggag ttagagagga cagactaaaa aatgtcacaa aggatgataa taaagatgga
    26341 ataaaaggat aattttaata aaacaacctt gccataaaca ctcaatcgca ttaaaatatc
    26401 gatgagtgtt tatggagggt ttattatgca aatggtaaaa acaatcaata gatatcctgc
    26461 ttggtggaca ttatattacg cccttagatt tatatatttc ataagcatac cagtgttatc
    26521 catttttatt cttttcggag tgctctccat tacatcttca agatatgtga cgcaagaaga
    26581 ttatatttat acttgcgtct gtctgttttt actaattgcc cctgcaatat tgatgtattc
    26641 ccgagcaagc tcaagaaaag acaaaataaa aaaaattgtc gcggagataa aaaacaccgg
    26701 tttttattca cctgacaaag aatatgaggg actgtctttt actcagggcg tctattttgg
    26761 cgtggatacc aaaaaaggca ctatgctgta tgctcgtgct taccctggaa atataatgga
    26821 tattatcggc tttgacattg acaacttcac acgcactgtg accgacgcca agacactaga
    26881 gatttataca aaatacataa atataccaat ggtctcaatt ccgtcaggat gcatccatcc
    26941 caaaatgatg gcggatacaa tgcatgcaat ggcagaaaga ggatatgatt accctgtcga
    27001 ctttcctcgc ctaattcagg agaagcgtaa ggagtgggag cagattgcag gcatgcctgt
    27061 agccgaagta ttttaagata agctccctcc ataacagccg gttcaaatga gccggctttt
    27121 gttcattaca acagataact tatgccgtta ccgataaatt cgaccaagtg ctttaatcgc
    27181 agcaattttt atttccatat catcactttg actcaatcta tgtaattcac gagtaatgtt
    27241 ctccgcctga caccggcctt cacctaatgc atagatcgca gcaagtgtaa cgtcttttga
    27301 tttactcttc atcatttcaa acaagaaagt attagctatc gcaggaggca tgctcataat
    27361 caatcctctt catgaaataa acaatggcac tcacttacaa acaggagtcc actatcgcaa
    27421 gcgactcaac ttagcaatat ctctcaattt gaatgaaaaa accagatggc atgtgacagg
    27481 gaaccgtccg catgatgtcc gtgaccattt aaccccataa gctaaccttg tcataaacgg
    27541 gcgcagaaca gcctgccaga cagacaacaa aacaaaagtc caagataaaa catgaatacc
    27601 ggtatctcaa tgcgaagaaa taatctccgt agaaaccccc atgagaaaaa tatcatatgg
    27661 gagaaaatcg caattatcac agccagcgca ttcattattt tcgctgtgct ctggcattaa
    27721 ttattttcaa cacaaaaagg aaaaaacaat gaatctggta gacgcctttg tcaaaaaagt
    27781 aatatccgag ccatatgaag aatatggaaa atggtggata gatgttgaat acatttcatg
    27841 gggtgttccc gggaaaacaa gactgatgtt tgagtcaaag gagcaggcac tggaagttaa
    27901 agaagggtac aaatttttaa cgtgagatct ctttttgtta aagggcagga agcggtaact
    27961 ccctgccctt agttacgttt ccaggcttat cagtcgacaa gttcccggat cagttccaga
    28021 acagcaatca gcgcgtttgc aacggcaact actgccctga gaacgatgat taacagatta
    28081 atcatccgct tctccatcac tggaggaaat caccgttgct tactcttaca ttgcctgtca
    28141 caacggcact ttcagtgtta gttgaaaaga gacttcctcc tgccagagca ctgaacacag
    28201 caccacctgt ttgttcagcc aggacttaaa atcttgcatc catagtcccg atggatatca
    28261 cggcagaagt gtcgccagac agagctaacc tgcggattat aaggatggtt gctatcaaat
    28321 gaacagctcg ctattataag aacgttagtt gaaaagagat ttcctccggt cagggccacc
    28381 cactctgtaa cggaacagta aaaaaaccga cctcaccagt cggttttttt acatctgttg
    28441 tttctcaaaa tccgcccgac atgcccgtca caccggggaa actcatgctc tgaatcagaa
    28501 ccaatcatct caactttgcc gtctcctgaa aaaactggca cattcagttc gataactacc
    28561 cataaataca gagagaaaat ccgatgtcag aaaagtggag taaagaattc gactcttgct
    28621 ttcaggtggg gagcatagaa gaaattgccg atgctctcga gaaacgcagc ggaagcgagg
    28681 aaaacgcgtt agccatgctg gcaatgacag ccttcacact catgacccga cgtggtatct
    28741 gcgaaatgca gaacgtggcc cccgatggca aaggcattcg gctggagcta atcggcttca
    28801 gggaaaatga aacaataccg gatacgcttc attgaagaag aaagggcgca atgaggtcag
    28861 ccttcgcaac aaagcggcgg cagaaaacct gccgccgtgt tgcccgttaa cgtttaactt
    28921 cgtaggctaa cgttgccaca agcactgtgt ttccctgctg gagccggagt acgcaaagcg
    28981 aatccctcac catccagaca aaacacagaa tcgtcacaca gatgacgatt aacatcatta
    29041 aaaacgttcg ctgtggcatc atagcccctc cattgcctta cggcggttat cgggctacta
    29101 taatgttgtc gaagcattac agtagagccc ttggttgatt gataaaaaaa tctccagggg
    29161 ctttctgctg tataccgtcc acaaaacgct ttcggcatac agcctcaagc gcccgcagct
    29221 attctaacga ctttcgtatc tgcaacaatc cttttatcac acggctaatt cctctggtta
    29281 gctcttatcg cctgcaaata aatttttaac ctttccgttt gatttaaatt ccagaaatgg
    29341 agactttgtc atgtcttaca atcgacagcc tgtcgcagaa gatcctatgc agatatgggg
    29401 cgctgttggt gtccttctta tcctgctgct attcgttata tggttattct tgccggaagt
    29461 ggtttacgcc agctgcctga ttcttcatac cttatgggga ctggttgact ggggaccatt
    29521 tcataactat gcagcaccac gatacaacct gcttgccatg accggtaaca acgccgccaa
    29581 cataagctat agccagtggg ttaacgtcat ggagcagacc ataggtattc tgtggatgta
    29641 cctcttacct gtgacactct ggtgtttgtg ggagtggtat caacatccgg gacaaagtcg
    29701 tttcacccgc agaccagtag atattacccg cctgccacac atctttgctt cgttgtcccc
    29761 tgctatcgcc cctgtactgg cagatggtga tccggaaaaa ctctttcatg gtggcaaacg
    29821 acctgaacgg cgggttgctc tcaccccgga agcatttgtc gagcagcata ctctcatcac
    29881 caatatgcag ctcgatgttg cagctgcaag acgatgtttc atggcccagt taggtaaacc
    29941 tttaacgtcc tggaaagaca tggcaccaca tgaaaaagcg ctctttgcca tatttggtct
    30001 tcagtatttc ctggatgatc gtaaggcagc attaaagctc atggatacac tgaacctctc
    30061 atgcagaatt aaaagcaaac gggacagtgg gaaattctgt acccctgttt attcactggc
    30121 caaatcagct ttccagagag tgattaaaag caacggagca cagcaatggc tgaaacaaca
    30181 tcgctacgtc cgcagtgggc tggtctggct gtatgcacat gacctgcggc ttacgccacc
    30241 caactggatt tggctgaaag gcgtggacag gacgctgttt tatgcattac accgcgccaa
    30301 taccacgaaa ggatttattg aaggcgctgg cgtggtggcc gttgcacgtg cagaggccga
    30361 agccatgcgt tttggtcttc cctgccctga accctgtgtt gatgaagcgg tggaaggctt
    30421 acggcgggat atgctcagtc tcggtcttat ctgggatgaa ccacaaccag atcgcgaccg
    30481 gaaacgccgc atcctgacca actggtccct caccgacgac atcctgcccc gcacacctgc
    30541 tacagacaac gaattctgat tatccggaga actgaaaatg acaatcgagt actttgcaac
    30601 acgcattgca aaacatattt ccgtgcaggc aatctggccg gatggcagga cagaaattat
    30661 tgccgtcctt ccacgggatg ccctgtcagg gctggtaaca cgaaataatc aactgattca
    30721 tatagccttt gacgccatgg gcacccgtaa cagagaaacc gtcctgattg ataaacagga
    30781 gcataacgct gaccagatac ttacagctat tgattcctgc ctgcgccttg aatatctgat
    30841 ggaacgccgc ttcagcagaa cacctttgtt ccgtggcata atcgccagcg ttgtgctgtt
    30901 cgtaatggca acgattggct tctctctgtt ccgctatgtt gatcgtgtat tctgggatga
    30961 tacaacgcct gaagcagtac agacagcagg tgagccacga ctgctacccc ctcacctgaa
    31021 tcacactgtg ccattaaatg aaggcattca actcccggtt cccccgaaaa aagacgtcca
    31081 ggtcccggag aaaacaatca caagcaaaaa tccggaagcc gcagctgcgc gacataatct
    31141 ggctactgtt ctgaagcgga acgccgatcg gggcatgttt accatcaacc tgtcatccgg
    31201 tcatgaacga accctgttcg catttctcga tcctgcctgt cctaactgca gacttctgga
    31261 gccggcctta aaacgcctgg ccagcgactt caatgtcgtt atctacccgg tttcagttat
    31321 tggtggagag gaatccaccg accgtgtggc accactgctg tgtgaaaaag atgctcagaa
    31381 acgtgctgcc ggctggcacc gactgtattc cgcagataac ggaatgatga cgccatcaga
    31441 agaaacaacg cctgcagatg aaacatgcct gaaagcagcc agagctgcga ttgatgttaa
    31501 caatgtcgcc ttccgtaagt tcggatttgc cggtacccca tgggtgctgt ccgacaccgg
    31561 ctggcatctg cctacaggaa ttctgcagga aactggcacg cttaatttat tcctgaaaac
    31621 caccgacagc gagtctggcc atgagtgaac atcgcgttaa ccccgagctt ttacaccgta
    31681 cagcctgggg aaatcctgtc tggaatgcac tccagagtct caatatctat ggcttttgcc
    31741 tggtggcaag tcttgtggcc agttttatct ggccacttgc attaccggca tgtctgttgt
    31801 tcacactgat aacaatgctg gtatttagtc tgcagcgatg gcgctgccct cttcgtatgc
    31861 ccatgacgct ggaatgtgct gacccgtcac aggacagaat gattaaaaga agtcttttca
    31921 gcttctggcc aaccctgttc cagtatgagg tcattctgga atcaccggcc agcggcatat
    31981 tctatgttgg ttatcaacgc gtcagggata ttggcaggga gttatggctg agtatggacg
    32041 atctgacccg ccatataatg tttttcgcca ccaccggtgg cggtaaaacg gaaaccatct
    32101 ttgcctgggc gattaaccca ctttgctggg caagaggatt cacccttgtc gatggcaagg
    32161 cacagaatga tacggcaaga acgatctggt atctggccag acgctttgga cgtgaagacg
    32221 atgtggaagt cattaacttc atgaacggag gaaaatcccg cagtgaaatc attctgagtg
    32281 gtgaaaaaac gcgtcctcag tcaaatacct ggaatccctt ctgttacagc actgaggcat
    32341 tcacagcgga gacaatgcag tcaatgctgc cccagaacgt ccaggggggc gagtggcagt
    32401 cccgggccat tgccatgaat aaggcactag tatttggtac taaattctgg tgtgtgcgcg
    32461 aaggcaaaac aatgtcgctg cagatgctcc gtgaacatat gactctcgaa gggatggcca
    32521 aactttactg tcgtggcctc gatgatcagt ggccggaaga agctatcgca ccgttacgta
    32581 actaccttca ggacgttccc ggtttcgacc tgtcgctggt caggacacca tcagcctgga
    32641 cggaagaacc cagaaaacaa catgcttatc tctccgggca gttttctgaa actttcagca
    32701 cattcacgga agcattcggg gatatttttg ctgaagattc cggtgatatc gatatccgtg
    32761 acagcattca cagcgacagg attctgatgg taatgatccc cgctctggat acatcagcgc
    32821 atacaacgtc tgctcttgga cgaatgttta tcacccagaa aagtatgatt ctcgcccgtg
    32881 atttgggcta ccgcctcgaa ggaactgatt ccgatgcact ggaagtcaaa aaatataagg
    32941 ggcgtttccc ttatctgtgt ttccttgatg aagtgggggc atactatacg gaccgtattg
    33001 ctgtggaagc cactcaggtg cgttcactgg attttgctct gattttgatg gcacaggacc
    33061 aggaacgtat agaaggacag acaacagcca cgaatactgc aacactgatg cagaacacgg
    33121 ggacaaaatt tgccggcagg attgtcagcg agggtagtac ggccagaacg ctgaaaagcg
    33181 ccgccggtga agaagccagg gcgagaatga acaacctgca gcgtcaggac ggcatatttg
    33241 gtgaatcatg gattgacagc ccacaaatca gcatcctgat ggaaagcaag ataaatgttc
    33301 aggagttgat tgaacttcat cctggcgagt tcttttctat tttccgggga gaaaccgtac
    33361 catcagcatc attcttcatc cctgacgatg agaaaagctg cagcagcgat cccgttgtta
    33421 taaacaggta tatcagcgtg gatgcgccac gcctggaccg tctccgccgt cttgttcccc
    33481 gtactactca acgtcgaata ccgtcaccgg aaaatgtcag tgccatcatt ggcgtcctga
    33541 ctgcaaaacc ctccagaaaa agacgaaaaa ttcgcacaga gccgcacacg atcgtggata
    33601 cattccagca acgcattgca ggacgtcagg cggctatggc aatgctggaa gagtacgaca
    33661 cagatatcaa cgcacgtgaa agtgccttgt gggaaacagc ggtgaatacg cttaaaacga
    33721 ccacacggga agagcgacga attcgctata tcacgctgaa cagaccagaa ctacctgaaa
    33781 caaaagagga aaatcagata tcagtgagag cagaaagagc aggtataaac ctgttaacgc
    33841 ttccacagga caataaccac ccgacagggc gtcctgtaaa cgggtttcat cataaaaaaa
    33901 ccaaccgtcc ggactgggac ggaatgtact gaacagtggc cgcgccggat tctggcgcgg
    33961 cctttttttt aacctggaga aggcggttcc cagcgtttat cctgctccgg cggaggcagt
    34021 gatgcgtcat cgttgctgtt cgccgcgata taatcgcggt agtagcgttc acttccttca
    34081 cggtgatgat ccacgtccgg tcgcgtaatc ctgtagtctt cgtgaccggt acggcctgtc
    34141 acattatgcc aggcaaccat ttcagcaaag acctggttaa attgtttgtc gtttccttct
    34201 ggttcaaaac ccatgcgtgg atcacgcccg aataataccg gtgcaatcaa atccagttta
    34261 tctgccagcg cattgcggtc atggtgatta cggaaaacaa ccctgtcacc ataatccgta
    34321 cagacaaact ctcccgtccg acggtcacgg aaacgaacac tcccgttctt ctgcaaacgg
    34381 gcctcgagat caccggtatt accgtatacc ggcgttccgc ccggttcaca aagcacgaca
    34441 cagcggtctg ttgtcgttgt gcgtgactta tctttacggc gatcgcgata atcccagcct
    34501 ctcagctgcg atacagcagc cctgtctccc tgagcagcct gaatttcgac ccactgacgg
    34561 taagagggtg gataccactt cccgtcagca ataagtttct gccgctcatc cctgatgtct
    34621 tctttcagcc tgatcaatgc ctgcattcgc tgaacttctg caatgtgata atgcagctta
    34681 cgtaatgccg gatcatcgta ctgcgcacga atgtgtgact tccgcagacg gcatgcctga
    34741 tgaatttccc gacaacgttc cccataacgc agatctggtt tacgccattg ttcacgccat
    34801 gccagatagc gggcacgcag gtcagcacgg gcctcagctc tggcttcacg tcgctgccgg
    34861 cgcagcattg ggtcacgttt actgctgacc ccgtacctgt cactgacagc cagctccggg
    34921 ttatagcgac tttcaggttg caccctgtca aacaaatctg ctggggcact cacaaaaggt
    34981 ccggcctgtg gctctgcacg ccccagagtc agatcaggat gaatgctgct ggccttcacc
    35041 ggcgtttgct catgattaaa ggcatcaacg acaacaagcc cgtgatgttg tttctgtagc
    35101 atcagtccct gttgtgcaaa caactcatga caacgctgcc aggtaaactc accctgtgtg
    35161 atcatttctt caatctgagg gctacaatgg ctgatgctgt atccccacag actttctttg
    35221 tcagaaaaca gttcgcgttt gatagccccc tgcggccccg tacctgtacg catacggtgc
    35281 cattctttat cggtcgcagg acagctctcc atacgacgac cacggaagcg ttctgttggc
    35341 gtaacctgga gaaaaatatc tgttggtaca ggctgccagc caccatcgag ttgattcaca
    35401 ttgtcgagcg tcagcagtga ccatacgctg tcagcacgaa cgggagtctg gttatgatca
    35461 taaccgtcac agataaccag gtgaccatgc tgaacacgca tccataatcc cgcttcagcc
    35521 agtgtgcggt gaatagcctg gcagttctcc agccgtcctt cccgtgccat ttccggcaga
    35581 cgttctccaa gatgacgaca ggcatactgc tgcagacttt ctgtttcagc gattttttcc
    35641 gggcgaacat cagctgcaat gaagtccgga ttatagcgtc ctggtgcttc cacctggctg
    35701 aaaatgtctt ttggtactgg cgtgtagtca cccatttttt tcatgagttt ttctgcacac
    35761 caggaagggc caaacgagtc aagctgtaca ccttccctgt tgcgatccca gccatccatc
    35821 accagaaatt ttccgtccat ctgagacagg tacagaccat cttccgcaag acgacgatgc
    35881 agggataacc agtcatgtac aggttcactg cgtaaaccag ctaccgctgt ctgcgcaata
    35941 tactcgcgaa aagtttgttt ttttccgcgt gtccacgcgt tctggcgatc acgttcaacg
    36001 gcggttttgc gaacgatacg atttcccggt gcatggaccc agcaaccgtt atccggagca
    36061 aaaccatgct tcagttcaag ctcacggcag gcacgactga gtttttcctg actccaggat
    36121 aaccggttca gataacccgt ttcagggtgt acccggttaa cagccacatg gacgtgcaga
    36181 ttatctgtat cagtatgcac ggcagagaca tactgatggt cggcaaggcc gagtgatttc
    36241 agcgtatgac gtacactgtc ataaatctgt tccggacgcg ggctttcatg tgactgccag
    36301 gaaaggatgt agtgaaaaac aggatcagtg tcatcttttg catagtgtgc ctgccgggca
    36361 atgtactcca tatctgcagc ggcagtttca agagaagtac agttatgaaa gcaggtgaca
    36421 ccataaaagt tgacccattc gcagccgtcc ttcatgacat caaccagcgc cacgaatgac
    36481 tcatttcgaa gacgtgtcgc ataatcaaca aggcgactga aacggcttcg gtgcggttgt
    36541 tcagcctgcg aagaagaaga taaatccagt tcctcatcgg tcatgtcatc ccggacagag
    36601 acgtaggata ctaggtcttc gaacgaagac ttgccgtccc ttctcttttt cggaatgact
    36661 gcattcacag gagaatcctc aaccttccat taactgttta cgcaatgtat ttgtaagttc
    36721 ggttatggca acaagcactt ctgcgtattc cttgtcaccg gttctttttc cctgaacaaa
    36781 cagatgcttc tgcatccttc ccagtctcat taactccttc agaaaagcat catctgtacg
    36841 ggagttaatt cgcttattca gtgctgcatt tcggatgtaa gcagatacag taagtccgga
    36901 atcttcagct tttttcctga tggtttcatc ctcaacagga gagaatctga gtgtcctgac
    36961 aactgtcttt tgccgtacct cacttttctt tcttaccgca ctatcactca cttcaggctc
    37021 cttacggggt gtcggggcga agccctgacc agatggcaat tgtaatagcg tcgcgtgtga
    37081 cggtattaca attgcacatc ctgtcccgtt tttcaggcca ttatagcctg ttatttttgt
    37141 gcatcatagt cttatgcaga cggcagaggc agggcgcagc cctgccagga gtcagttttt
    37201 gatgataaaa tcagacttca acatgaaacg gagagaaagt gccaatggct tctaaaaaat
    37261 tttactcaga tgatgacatc cagctggcaa aagctgcttt atcggaattg ccagatctca
    37321 ccgctcaacg gaaaacattg cgtgattttc ttgatgccat tcgtgatgac attattgttc
    37381 tggtaaggac aaagggttat acactggcag atgttcgtga cacgctcaaa aatgcagggt
    37441 atgaagtagg agagaaggca cttcgtgaca ttattcggga agcagaatcg aaaaagccat
    37501 cccgccgttc ttcctctaaa acagcatcta aaaagacaga ttcagccagg aaagatggca
    37561 tcgatatgaa caacaattga tgtgacaatg tcacatcatg ttctgatatt atgatgatta
    37621 cagaaaaaaa gccccggcat aaataccagg gcttttctgt ttaatcagac tcgtggcggc
    37681 aatgaaccag gataatgccg tgcaataata tcattcagtt tttcaacaag gtcaggacgc
    37741 ttaaagacaa tgtgtacggt ccctttctgg tagccacgca ccgtaaaata ttccccttct
    37801 aacagcttac cgacattgtc gccgttcccg ataaattcac cgtactgcgc cccgatgctg
    37861 ctccggaagt caggaacgtt gcgcccctcc agcagataaa acggacgtac caggtcatcc
    37921 agtgcctccc gtccgctgca gtccagcgta acacgcccgt tactccagcg gtacagcagg
    37981 ttttcaataa tgattctctt gcccagacgg cagggattat tggttctgta atcccatgac
    38041 aatttccggt agacatcaat cagtccctgt tcaaatgtct gcatcttact ggcattcaga
    38101 tggcgaaaag ttgccagtac attgtccagg gtgatttcag gacaggtgtc gctcatcagc
    38161 tggctgttcc actcatcacg ctgacatgaa ctcatgaagg tgtacatccc cgtatccgtc
    38221 atcaggcggt cccagatttt ccggtcggca agacgggtga tattcctttt cgcttcttcc
    38281 ggtttatcca tgcaccgaac ggagtcggtc acgcagtctt caaaaccgta aagaaaaata
    38341 tcacctgacg catcaagcag aatcctgcgc gctgtcgcca ggcattccat cgcctgcatg
    38401 aacagtgcaa taccttcgtt acgctgcgcg atgatgcggt cgatggccac agacggaata
    38461 acatctgtcg tgccggcagt attcaggtca gcagtgggta aagtctgatt catcacattg
    38521 tctcctcatc gtcacgactg ccgggtcagc caggtcattt cccggaggag cgaagctcct
    38581 tctgccgtcc gggtcttcgg ggatttcccc gcagtggacc tttccgacgg gggacgctca
    38641 ccacgtcacg gggcagttgt ccgggggcag acgcaaaagt ttttgacgcc tgcggcaaaa
    38701 agttttgtgg ctggtacatc tctcccctgg acgactgaaa cgggatgtgg tcgtgtatgt
    38761 acccccggaa agtccatctg cggagaacag gccgcagtgc ggcggcagac aggcagagac
    38821 agctgcgcca tcaggcgcag tggccttaca caccgtaagg tgtgcaggga gcgccgcatc
    38881 cggcgcgact gaagaaaaac gacggcgcaa caatggcatg tggtggtcac tggcacacca
    38941 ctcccgcaca ggtgaaaaca gagaaaacag cacgacacct acaatcaaaa aaagtgaaat
    39001 tccttcactt tttcacactt atttttcgcg ctgatttggt ggttatggtg ataaaataaa
    39061 gtgaagtttt ttcacttttt caaaggtgtt ttatgaccgg ctgggaactg cgaatctggc
    39121 gaaaaagcat gctctggtca cgcgaaaaag cggccagaga atttggcgtg acacagagaa
    39181 catggcacgc ctgggaaaat gcggaacagg tggatgtcac cgtctggagg accacccagg
    39241 cactttcagt ccgtgacctg ctgcctcaca tgcaggggat gcgcaaggca gatatcatca
    39301 ggcgtctgga aaatgaactg ggggaaacgg cagaggatgt gtgagcgtgg ttcacagcct
    39361 ggcagaatgc caggctactg ctcatcagga aacagttttt tcagttcttc accggaaagt
    39421 cccgtggcct cctgaatccg ttccggcgtc cagcccatat cccgaagccg gcaggctatt
    39481 tcccgcgcca cttcaagagc acctttttca aaacctttct gtataagacg ttctgcaatc
    39541 gtcatactgt tttctctgct cctgctggca aaaggcccgg cattttgccg ggccttttgg
    39601 ctctcatcag tgagtgatgt tcttcagttc agcttcagaa agtccggtca gctgacagat
    39661 ttgtgctgcc ggcattcccg cgttccggag ctcccgggcg atttcccgtg ctttttgaag
    39721 cgccccctgc tgaactccct gctggatacc ctgctggata ccctgctgga taccttgctg
    39781 gataccttcc tgcttaagac gttctgccag tgtcataatg ctgtcctcgt gctgcggcag
    39841 tcgctgcgcc agcgtctgca aaaattcaac cgttacatgt tcgctgccat taagcagata
    39901 gttgaaaaga actgtaatct gctcttcatc ctggatgtcc tgcagtgctc tgaccaggtt
    39961 gtccagacac atcagcatat cccgctgacg gatatgcttc atcagcagcg tcagtgcggc
    40021 catccggcga tggttcatga tttcattgtc atcaacgaca gtgatatcaa ccagcggaaa
    40081 cggctttgtg tatattttag ccgcaagcgc cggattctca aaacagtcca gccagttcat
    40141 gctgtacggg tacgggctgc gctcaccgtg atagaacagc accggaatga ccagtggcaa
    40201 cgttttgtgc tgctccagat gtctctgcat cgccgccaca ctgtagcgta acatgcgaaa
    40261 aggcatgtac agattttcgg tgctctggtg ctcgatgagc gtataaacat aaccatcttc
    40321 acccccggtg gttttcacgc tccagaggat atcgctggcg tactgatgca ggtccggctc
    40381 aacaaaggtg gcgggttcca gcttcagcgt ggacaggtcg cacaactgcc ggtactccgc
    40441 aggaagatgc agttccagaa aatctctggc gacgtcggga ttcgccagga acgaccggaa
    40501 cgcggcatca tgcggcgtgg gcgttgtggt gtttttcttc ttactcacgg ttcctcctgg
    40561 tgagatgtct gaacgtatcc tgccgcctgc acaggcagac ggcaagggtt aatcagtggc
    40621 agacccgctc aatttcaagc gcgcggagca ggtggcccca ttcgctgact ttcatagcgg
    40681 ggatacgcac ccgcttctgc tcactgcagc gtctggcgat atggccgctc atcgccagga
    40741 cgtccgggtt aatctcatcc tgtgccagct cctgacgtct gaagcgggta tggcggtgca
    40801 ggggtttatt ctcccgcagg cactcttcac gcagacgctt tacatactcg gcggttgcga
    40861 agataattga tggtctcaca atattactcc ttatgttgtt gatgactgcc gctcttcctg
    40921 gcggggaagt aacggcggag agaggcaggg tttatcgccc tgcctttttt tcatttcgga
    40981 cagcgacgca gtgccagcgc gagcgctttc atcttccagc cttccggcgt gcggtacatc
    41041 accttccagt agccgtcgat gaacacataa tggaagcgca cctcttcagt gctttcagtg
    41101 gcaatagcct ccctgaaaga acggtactca cgcgcctcac tgtccatccg gaaaccactg
    41161 tcacgtccgt aataagccgt cacaccgtcc accgggttct gaagcgtatg cccggcgggt
    41221 ttgtcacaac ggggaccgag gcaacggata tcaccgtgtt caaccagggc ttcggcctgc
    41281 tgaccggtgg catagtgagt atgcaggatg cgaccgttcc aggtcggctc cccgtgcagg
    41341 tggcagtaaa cggtacggaa caccccgtcg cggcaaaaaa cggaaatcgt gctgctgact
    41401 gtcatgaaaa catctccttc tgctgaatca ggtcgccggg ccgaccggtt gctccccgga
    41461 ggggcgaaag ccccttctgc cgtccacttc ttcggggttt ctccccgcag tggacctttc
    41521 cgatggggcg tccgtcacca cgtcacgggg cagctgtccg ggggcggacg caaaattttt
    41581 tgccgggttt atggctaaaa attttgtgga cggtatatct tcccctggac tgctgaaacg
    41641 ggatgtggtt actccagccc ccggaaagtc cacagcggga aacaggccgc aggggtggcg
    41701 gcggaagggt tgactgcgcc atcaggcgca gttgccttac acaccgtaag gtgtgcaggg
    41761 agcgccgttt ccggcgcgac aggggcggcg cgaccggaac ggtcgctgca agcctggttt
    41821 ttcagacatc ggaccgtagc ggcagcccga aaccgcgcca gcgggttcgg cggagactgg
    41881 cgggtgcgcc agcagctgcc tgtcggagtc gggcctgacg accggatgcc gccaggcagc
    41941 cgggagccga aggtgtcccc gcctccggaa atcagaacaa cagaaacgac ataacagaaa
    42001 aatgcctcac accatgacgg gcaggaaggg cagcgccgca ggcgcggccc tcctgcattt
    42061 caggcaggcg taagcctgcc agggttttcc ggcggcctgg caggccatac ccccgccgga
    42121 gcgggggagt aaatcaccga cggaacacat gaccgtcaat aaaggtgaag gagtccagga
    42181 ataaatcccg cgcatacgcc tcatagtcaa aatagagcgc caccgtctcc ggcacgtcag
    42241 ccagcaggcc ggtatcactg gcgaactcaa ccgcaaaagc ctcctcactg tcagcctcgc
    42301 cccaccaggc atcgcggaag gtgtcaaaat ccgtctcacc ggtatcatcc acccagagac
    42361 gataagcctc ttcgcagcct tcatcccgcg cctggcggaa gccttcaaca taggcccagt
    42421 tgatatggca ttctgaggcc atattccccg ggaatccctc ataatcctga aacatcagtt
    42481 caggatcggc ttcatcctgg tgaagagagc ggcaggcggc gaaaaagtcg cgttcatcat
    42541 caaacgtggc caggtcaaac cagcggccag cgatgcttcc acagttgtat ttgtgccagg
    42601 ttccaacgta tacagcaggt gcaacaacag acattcccga atcctccata gtcacatgtt
    42661 ctgcgacacc gggcggtgcc gtgcctccat aaaagccggg cgataaggcg gggatgcaca
    42721 gggcgcaagg gaagccgcag cctgacgcac acggctggcg ggagttggag ggtgagaccg
    42781 cgaaatctga gcgggtgaac gaccggaaaa tcacggcaga cgggaaggag ggaccgggtg
    42841 accgcagcgg cgaagcgtac ccttgcatcc ctgccagccc ggctaagatg gaggcacggc
    42901 acagcccgca cacgacgctg aggccacaga gctgaaaacc cacaaggcca tgccccgcat
    42961 ggccgggtaa aagcggagcc gccggcgact tccttattcc ggcgagtgca gaggacacgc
    43021 cgcatgacct ccagaccacc cctgatatga aacgatcatg atcggcgtgt ccctgcgtaa
    43081 aatatacatg tctggttcac cggagaatat ctgtgatatc cacaagtttt ctgtcgcaca
    43141 gccagtcctg cgcttcacgc accacgctac tgtcgggttt caccgctttc agcgatgatg
    43201 ccagtcgctt gatcaacata accagttccc gtgtctgtat gtccggcatt gtctccggcg
    43261 gagcagaggg actctcattt ccgacgccca tcccatgcgc ttccagtgtg gcacggacaa
    43321 accaggcggc atcgaccagt tgctggcgaa tccaggtcag atcggcattt ctgtcacggg
    43381 ctgccattcg ctcacccgac aacgccttgc tgatatggct gcgcaccaga ttatccagga
    43441 gttcctgcgc ttcttccggt gaaaagccac ctggtatctc tgtgcgcaac agtcctttcc
    43501 tgtacagtag ctgcagcaga tcaatctgcg accgcactgc aactatcagt acataacgga
    43561 caatattcgc actggcggtg gtttctgtat cattcagcag ttcctccgga tcattaccac
    43621 cggtattgca gagcaacggc aacagaggtc gcatccacac gggaacctga tattgttcgt
    43681 ctgatttcat catattgcgt tctccagctt gttcgggatc atccactgct caagtgcacg
    43741 tacctgtacc ggtgcaccac gaccggcctc accggcttca aaaatcagcc gccagagtgg
    43801 ttcctcctgc cagaaaaacc ggtcaaatga tgacagtgat ggaaaacgcc cgcaccaccc
    43861 ccagaaaatc gtttccagat catgctcctc aacaacacca gaccagcggg acaaccagct
    43921 tccgaccgtg gcgggagtat gaaacgcaag gtcaatctgc gcctgagcaa aacatgtctg
    43981 gtattccagt tcccgtcgtt ctgcctcacg ttcccgctgg cggctgtatt tctgctctgt
    44041 cagcgtggtt ttctgacgac gccgctcacc ttcacgaaaa ggaacggacg ggaacagacg
    44101 atgccccgca tcggatggca ggggaagaac acagacttca ccaaaacttt caatcagttt
    44161 atccacggca gccagccaca ggagacggtt gccatgaaat tcctccttcg tcagcgcagg
    44221 agcaaaaaag cggagatcgg tcagcgaaat ccggcgactg ccattcaggc agcgaaagaa
    44281 agcgctggca taaggccatg ctgaaagtgt gcgtccctga cgacgacgtt gttcggtgac
    44341 agccacagcc tgcatggcgg cctgttgctc tgcgctgagg gggatcagtg cccgtgaacg
    44401 tgcgctcatg cctgacctcc ttctgccatc aggatatgga tgatgtcgtt aacactgtac
    44461 ccctgcgcat caagtgtcgc ggcaagcgcc agcgtgtgac tgatagcgac aggcgtgaag
    44521 atatgttgca cctgaacgac ggcgaaaggc tgtccaccac aattcaccag aaccagcatc
    44581 cagagctgag agacgtctcc cggcgaacat aatgccaggt ggaaacgttc gtgggctggc
    44641 gtgaaacgaa cctggacggg gaaaaagccc ccgaactcac tgccatactc gccattcatc
    44701 atccagttag ccggtgcgtc cacttcacgc atcacctcgt gagtgagatt acggcgcata
    44761 tcgcttcccg cagcacggat atcttcatat tcctgtgcgt tcatggactg cagggcattc
    44821 agggtcagtt cagttttcat cacggtatct cccttgctgc cgccccgtct ccggcggggc
    44881 ggtgtggttg ttcatcaggc ggcatcagcc aggttatcgg tgcgttcagt ggtatccgtc
    44941 tgtggatgtg gtgcacacat ccagcctgga acccagcggt tgtctttcat atggtgctct
    45001 gcatgttcag ccgcatcacc tttcttcatc ttctccgcgt cccgtgcggc accggacagt
    45061 ccggcatcat tcagggctgc gataatctgc ggctttttca ggtgtccgaa gaagtttgct
    45121 ttcgtcggct gccaccagtc gcgcatgtga aaaccgatgg cgctctccag cgaatccagc
    45181 ggactgcgtg acgtgtgacc acactcgcgg gtctggacac cgttaaggct acatgcggtg
    45241 cagaaactga gcagggataa cagcacctcc tgactgagtg acaggaacgt cgtcatgtcc
    45301 cgtgaccatc cctccggtaa cagggccgca agacgggctt tttctgccat cagcaccagg
    45361 aatgcggcac cttccttccc tgacggcgca tcgctggtca gcgaataatg tttacattcc
    45421 agactgattt gtactggttt actgtacgct ccgctgccaa acacattcag gcagagcgtc
    45481 catgccaaca gtgccagaga tttgtccggc tgctgcatca gtgccgcctg gactgccagc
    45541 gtgcgttcgg aagacatttt cgtcagcaac ggcaggctga tttcctccac tgacgctttt
    45601 tcctgcacct gttccgtgcg gtcagcgtcg tcagccgcat catcctcact gcgcaactgc
    45661 acaccacgct ggacacacac attaccgtaa cgccaggaca ccaccacacc gcttccggca
    45721 cgcatctccg gcgtccacgc tctgaccttc gccatgcagc gcatcagctt catttctgct
    45781 tccagcaggt cggattcctc acactggttt tccagcgcgt cgtaacgcgt catcagttcg
    45841 ttcaggcgtt cgtcttccgc ctccgtcagc accgcttccg gctccggcag acagcggtat
    45901 gttccggcat cctcacggca ctcaccgaca ggctccatac gtccggcgca ccattcccag
    45961 ccttcggctt cccgaaggaa ctctgcaaca gcctggagtt tttccagcag ggcggcatcg
    46021 agcgccacgc ggtcgacata accgtccccg tcatcgctga acaaatcggt gcgcaattcg
    46081 tctggcgaga aggcatcagc ccccacaaag cggaatttag tgttgtcctt cactgccact
    46141 tcactttccg taatcaggtt gcggataacc cgcacatccg gtttaccgcc ccatcctgac
    46201 tggcaggccg cttcaaacac ctgcacctga cgcgcggtgt cgctctccag cgccagcgcc
    46261 tgacagtgct cggtggtgat gcggtcctct gccagcgcat cgaggatgac aggcgcaagg
    46321 tctgccagtt tcagcattcg ctgaacgtgg cggggtgaat agcccagcag gtcgccagtc
    46381 tgtgcaggtg ttttgccttc ctgcgccaat gcgcggaatc cggcaatctg ttcggcaggg
    46441 tgcatatccc gacgatgacc gttctcggtc atcgatgcgg cagtcgccag ttcctgcggg
    46501 atgaccttca cacggacagg ccagtcagcc ggaaggatgt tgcgctctgc cagcatgttg
    46561 agtgcagcca gtcggcgacc acctgcggcg acaccgtaac ggtcaccagg cagggtatgc
    46621 acgaccagat tctgcaacag gccaacgccc ttaatggacc cagccagttc gctgacggac
    46681 tccacagaat acggcaccgt gcgcacattc agcggtgatt taatcagtga ggccagcggc
    46741 acgctcactt cctccgtctg cgccagcagg gcagacaggg cagtttcctg cgtttttgca
    46801 gatttacggc ttgctttgcg ggtggtttct ggttcacact tcgttacagg cataatcatc
    46861 cccttataat tgcttatgtc ctgccggacg ttcgcgcgtc cggcacctct cttacttaag
    46921 aatcccgaaa tcacggatgg catggttcat ccagtacccg gcaccgtcct caaaattcca
    46981 ggtacgccag accattgttc cgttattacg gacaaccagg cgaaaatgtt ctccgtggtc
    47041 atcctcgata aagacgttcc ggtactgtgc ggcaacggct tccgcctgtt cgcgggtaaa
    47101 ggagccgtcc ggcagtccct gaagtattct gaaatattcg ctcatcctca tatctccggt
    47161 gatgccccgc acagggcggg gcggttgtca cagtcagccc gatcagaacg ggatgtcgtc
    47221 tgaaaacccg taatcctccc cctccggcgt ttgcggctga ggctcgggct gcgcggcctt
    47281 acgtccacgg cctttcgttt tcgcgccacc ttttttcgtc gcgtcagcac tctgtggctg
    47341 cccattctgc tgaggcttcg gttgcgcctg agcgttctgc tgtggtgcac tccccagcat
    47401 ctgcatggtg cccgtggtct taacaagaat ttcagtgatg tagcgggtga tgccgttgtc
    47461 gtcccagcta cgggtacgga gttgcccctc gatgtagacc tgcgcgccct tgcgcagata
    47521 ttcacctgcc acttccgcga gcttgccgaa cagcacaaca cggtgccatt ccgtctgctc
    47581 ccgcatctcc cccgtctgtt tgtcgcgcca gctttctgac gtggccacct gcaggtttgc
    47641 cactgcgcca ccgttgggga tgtaacggac ctccggatca ttgcccagac gcccgacgag
    47701 gatgaccttg ttgataccac gtgcactcat aagaacctcc tgactgaaag atgtgagata
    47761 aaccggatac cggagacggg acgttcatgt gccccttcag aggagcgaaa gctcctcctg
    47821 ccgtccactt cacaggggtt tctccccgca gtggaccttt ccgatggggg cccgtttacc
    47881 gcctcacggg acagctgtcc gggggcggac gcaaaatttt ttgccgggtt catggctaaa
    47941 aattttgtga ccggtgcatc tcaccctgga cagatggaac gggagggggt tacgtctgcc
    48001 ccccggaaag tccacagcgg gaaacaggcc gcagaggtgg cggcaggagg gaagactgct
    48061 gcgccatcag gcgcagtttc ctcacacacc gtcaggtgtg cagggagcgc aggtcacggc
    48121 gcgacagagg cggcgcgacc ggaacggtcg ctgcaagccc ggtttttcac gcatcggacc
    48181 gtagcgacag cccgaaaccg cgccagcggg ttcggcggag actggcgggt gcgcaagcag
    48241 ctgcctgtcg tagtcgggcc tgacgaccgg atgccgccag gcagccggga gccgaagtgg
    48301 gcgcaataaa agacagccag aaacagtgac agtgtcacgg aatatcgcac ctgttcctgg
    48361 catacagcca tacactatct tctgaccgga aagccgggcc ggcagcgccg caggcgctgc
    48421 accccgcatt tccggcaggc gtcagcctgc caggggtttt cgcgcgggct gacaggcggc
    48481 ctccaccgcc tgccctgtat cacctgtttc tgctgccctg aaggatatag tgaccaccga
    48541 ggaaaaccgc ctgctgattc accacaaaat cgcagatttc atcaacaatc tgcgcagtgg
    48601 tgagcggttt ctgccccatc tcacgacgcc gcgcattaat cacggctttg taattgcgca
    48661 gaatttcgcg ggtttctgtg ctgatattca tgacatgtgc tccccgtcca gcgcctgtaa
    48721 cacaacgtcc atcgcttcgc gttcttccct gacgaatgcg tcaccgtcgc ccccgttctc
    48781 actcccgacg gcatacaggc gcttcatttc actgcctgcc agcgccacca ctttttccag
    48841 catcaccgcc gggactgtca cctgacggct gacgcgcttt tcttccatca gttcacgaac
    48901 aacgtcgtga acaacgctga catccgcacc atgctcaaag gcatcgtcca gccgctgcat
    48961 gacggtttcc agctcatcgt ctgacaggtt ccagtcctgg gctatgtgac gcacatcccc
    49021 acgggtcagc accacggcaa aaacgcattc actgtccggc acgtctgtat ccagcacttc
    49081 gcggataatt tctttaacag tacagtacat tttctcgtct cctttacagg ggcggcattg
    49141 ccgccccgtc cgttaatcag tccagagccg cgtaaatcgc gttgcattca gggtgaaatt
    49201 cgatgtgacg ccacaactgc gcatcgcgca tccggtaaag ctgggtcagt cctgcatcac
    49261 cgctgtcgtg gtacagccac aactggcggt taatcacaag ggaagtcaga atgatgccgc
    49321 aggcatcagc actcacggta cggtcaaacc agtttgcacc gttcactatg tggaaacggt
    49381 cgccatccgg catcatgtag ccgccaccgt ccggcaggcg gatgaagtgc cagaatccgc
    49441 cttcatattc cggcatcatt tttttcgcca gcgcgtacac agacgcttcg acatacagaa
    49501 aatcctggcc aaacaggtaa ggcagaaaaa acagacggtc gcactcttca acattcagag
    49561 tgacaggttt cgcatattgc atgatgtatt cctccgttgt tcacatattc cgcgacaccg
    49621 ggcggtgccg tgcctccata aaagccgggc gataaggcgg ggatgcacag ggcgcaaggg
    49681 aagccgcagc ctgacgcaca cggctggcgg gagttggagg gtgagaccgc gaaacctgag
    49741 cgggtgaacg accggaaaat cacggcagac gggaaggagg gaccgggtga ccgcagcggc
    49801 gaagcgtacc cttgcatccc tgccagcccg gctaagatgg aggcacggca cagcccgcac
    49861 acgacacggt ggccacataa ctgaaaaccc acaaggccat gccccgcatg gccgggtaaa
    49921 gcggagccgg cggcgacttc ctttgtgctc tgtccgggga ccggcaggaa aaaggcgggg
    49981 atttaccccg ccagtgtcat catttccgga tttttgccag ggcatccgcc atgctgcgga
    50041 tttcggtccg ggcttcccgg accacctccc gcagaacacc acggtcaaac cagcggcggg
    50101 gcggttcatc gggtatacgc agcacatcgc aggccagccc acaggacagg atatgctcaa
    50161 tacaatcgct gaccaggtac atttccaccg acgaaaaacg tgctccccgg ttcacgatgg
    50221 catcgagcac cacgggcagg ttttcgtcat cttcatcgag ccagcagggg tgatcgcttt
    50281 ccttcacgac caggcgaaag cggcgcgtct ggcgggtcag caacgtctga ccggtggtgg
    50341 tcagcgccgc acggatacgc cgcgcatctt ctgccacctg gtaacagatg cttcgtaccg
    50401 catcgtgcac cgcatcaaga acagccctga tttcgtcttc ggtcagccat tctccggcat
    50461 acgggaaaga cacctgcgtt ccgtctgccg gaagtaccgg cagcgactgc cactgactgc
    50521 cggacagtac cagttccgcc ccggttccgg tcagtctctg aatggcggag gccaggagcg
    50581 gcggcagtac gccgccgtga cggttgataa gtaacgtcgc cagccggggc gcatccagcc
    50641 gcacgtaaat gctgatccag cccggatagt tcggatttcg ctggtcaaca tccgtctctt
    50701 ccggccagcc ctgcgccagc agcaggttac gggcgtgacg acaaaccagt gcattcagcg
    50761 ttttgttcat gattaagcgt tctccctgta tcgccgcccg cagggcaggc ggcgataaca
    50821 tcagttgttt tcgtgtgtgt tgtttttcag cagggtgcgg gcctgattaa tcactgcctg
    50881 acggcgggta acacattccg ggacggcaag gccgttatca cgggcggccc attcacgttt
    50941 ccacagtgcc tgttcagcgg tctgaatcag ggacgccagc agttcagcag ggacggtcac
    51001 ctggcggttt tctctcacgt tgctgataat ctccatcgcc acgccggaag aaataccgga
    51061 ctcaatccgc tggtcttccg ggatgtcctc caggcgcgcc aggacggtcc tgatttcatg
    51121 atcggacagg gaaatatcca ttccgtcagc gagggcctga atctcttcag gggaccagac
    51181 aaccagcatc agtggcgtgt ctgccggata ctttgctgcc agctcccggc agagtatttc
    51241 gcatgttccg tacatactct ctcccctcaa tctttgaagc aggaatccag ttccagaatt
    51301 tcggtatatg cctccatcag gaggcggttt gcgcgaaaca acgtctgacg caccagacgt
    51361 tccggcaggg tgtcagcatt aaagctgtac cgccgctcca tctcattcat ggttgtacgc
    51421 aggtcgcaca tggcattggc cacctccgcc acttcggcgc gaagtttttc atgtccggca
    51481 tagttcattt acgcagcctc cggcataaac cagtcatcat ttgcggcccc ctgctgcatg
    51541 gcccgctgta cggcagccag acgttgctgt ccggcacggt gatactgctc aagcagctcg
    51601 ataccgatat accggcgtcc ggactgaagg gcagccacgc aggttgagcc gctgcctgca
    51661 aacgggtcca gcacaatcgc gttcggatgt gtgaagctct caatcagtgg ttgcaggctg
    51721 gtaacaggct tttccgtcgg gtgatgacga ttgcctgaat atttccagcc cagcacgtcg
    51781 ggtaatggct tctgaggcag cgccggacgg ccttttgcca gaacgtaggc gcattcgtgg
    51841 cgataaccca catatgcggc cttcgatgtg tagtttttgg tgaagaccag gtgcccaacc
    51901 acactgaatc ccgcattttt ccaggcggcc ataaagcgat cgacgcggtt ccagccgtag
    51961 aagctgacca tcagcgcatc ttttttgagt acgcgataca tttcattaca ggctggttgc
    52021 agccattcat cggttttatc gccggcgatg gtacgcccct gtcggtcacg aaaaccgacg
    52081 aggtatggcg ggtcagtcag tatgaaatca atgccgttat caggaatacg cgccataaca
    52141 tcaatgcagt taccgaggac aaaacgggac ataacaacac tccttctttc tcagagggca
    52201 ccgggagggc acccctgtgg tgcccgttcc ccgctgcctg tgggctgttc accgcgtccg
    52261 gtaacgggtc aggccgaccg tggaatatcg cagccgtcgt ctttttgacg gcgagaatat
    52321 gccgcgaaag cggcttgaag ggcgttcatc agaaaagggc gacaggctgg cgtacactgt
    52381 caccgacgcc agccgggagc ggtaaaagcg cccgtcccgt gcccgccgga cgcgggacac
    52441 tgaccacagg cagcgtttat gccgcgccgc atcagccggc gcagtaaaca gaggagcacg
    52501 gcgacatcct ttgtcggaaa agaaacgggc ggttgcccgc ccgtctccgt catacttcat
    52561 cccccgtaat gcgccacctt gtcgactatc cacgcaggtc cggtgacttc cggcagctca
    52621 tcgtcatctg tcggggaaga ccattccagt tccccccaga gcacatcaac gagctctccg
    52681 cgctcataca actgccagcc acagaaattg cagccctgtt cggcgtacca gtgctccagc
    52741 gtgcagccga aacggcggct taatgctgca acaacgtcgc tttccggctg acaccacggc
    52801 gtgtcgaaat ccacctgaat gcagttgtct ccctgactac tgatattcag gtcataccca
    52861 caaggccact tcacgccata ccgttctgta taccagtgat atgcagaagg aacaccgttc
    52921 agaacaccac cattaaagcc attcacttca acatccaggc gggtcggcag aaccgtcagc
    52981 aggtcaaacg gcatggtttt ttccggcact acgttgtcac acagacggtt ccaccacacc
    53041 gatacatcct cgctacccca gatgtcacac cagtcacctc tttttgcggt gaaatgcaca
    53101 gtgatggttt ccctgacatc atccggtaat ccttcccagc gacgctgtcc agtaccgctc
    53161 tgcagccaga gttcatgcag catcaggcag ttttgctcat ccagcagtac accgttctgc
    53221 aggtgtgtca gccagcgggt gaacgcgata ttttccgtcg atacagcacc acgtccggca
    53281 gccgtcactc cggggcactg ttcagagcgg atattttcag tgatctgcag aagtccggca
    53341 cttccggcca gaaacagctg aataccttca tttgtggcgc ggcgataaag cggtgtaact
    53401 gcaccgctgg ccagtcgttt aatctcagcg atctgtgccg gttcaccaga aaaatacata
    53461 cgattcgagc accagtttgg catgggaatt ctcctctcac atgcggcctc catcaggccg
    53521 tgccgcacct gttaccgggc gaccgcatac gaatgcaggt gcggccccga agccgcagcg
    53581 cgtggaggag gaacgacgaa acgtgaccgg gtgaggaggc cggcgacagc gacacacttg
    53641 catcggatgc agcccggtta acgtgccggc acggcctggg taaccaggta ttttgtccac
    53701 agaaccgtgc acaaaatgct gtggataagc tggacacagc agcccacagc aggcatacag
    53761 ccacacactc agagaattga gcgtttttta gtcacaacaa tgacatttga ctgtgtgcgg
    53821 attcagttat tagaatactg tatatacata cagcaaaaga aaagggagat gagaacatga
    53881 tccgcattga aattcttttc gaccgccaga gcacaaaaaa cctcaaatca ggcacacttc
    53941 aggcgctaca gaatgaaatc gaacaacgtc tgaaaccaca ctacccggaa atctggctgc
    54001 gtatagacca gggcagcgcc ccgtctgtat ctgtcacagg agcccgcaac gacaaggata
    54061 aagaacgtat cctgtctctg ctggaagaaa tctggcagga cgacagctgg ctgcctgcag
    54121 catgagtacc gtttatcacc gtccggctga cccttcaggc gatgacagtt acgtgcgacc
    54181 gttgtttgcc gatcgttgcc aggccggttt tccttcgcct gccactgatt atgctgagca
    54241 ggaactggat ctgaacagct attgcatcag cagacctgca gccaccttct ttctgcgcgc
    54301 cagcggtgaa tcgatgaacc aggctggcgt gcagaatggt gatctgctgg tagtggacag
    54361 ggccgaaaaa ccacaacacg gggacatcgt tatcgctgag atcgacggtg agttcaccgt
    54421 caaacgactg ctgttgcgcc cacgcccggc actggagccg gtttcagaca gcccggagtt
    54481 ccgcacactg tatccggaaa acatctgtat ttttggtgtt gtcactcacg tgatacacag
    54541 gacgcgggag ttacgctgat gtttgcactg gctgatatca acagtttcta cgcctcatgt
    54601 gaaaaagttt tccgcccgga ccttcgcaac gaaccggtca tcgtactcag caataacgat
    54661 ggctgtgtga tcgcgcgcag cccggaggca aaagcccttg gcatcagaat ggggcagccc
    54721 tggtttcagg tgagacaaat gcgcctggag aagaaaatac atgtattttc cagcaattat
    54781 gcgctgtacc acagcatgag ccaacgggtt atggctgttc tggagtcgct ttctcccgca
    54841 gttgagccct actcaattga tgaaatgttc attgatttgc gggggataaa tcattgcatc
    54901 tctccggagt tttttggtca tcagctcagg gaacaggtaa agagctggac aggactcacc
    54961 atgggggtgg gcattgcgcc tacaaaaacg ctggctaaaa gtgcacagtg ggcaacaaag
    55021 caatggccac agttttccgg agtggtcgcg ctgacggcag aaaaccgtaa tcggatcttg
    55081 aagctactgg ggctgcagcc agttggtgag gtctggggag taggacacag actgacggaa
    55141 aagctgaatg cgctgggtat taacacagca ctgcagctgg cgcaggctaa cacggcattc
    55201 atccggaaaa acttcagcgt cattcttgag cgtacggtac gcgaactcaa cggcgagtcc
    55261 tgcatatccc tggaagaagc accaccggca aaacagcaga ttgtctgtag tcgcagtttt
    55321 ggtgaacgaa tcacagacaa agatgccatg caccaggctg ttgttcagta tgcagagcgg
    55381 gccgcagaga aactacgtgg ggagcgtcag tattgccggc aggtgacgac atttgtacgg
    55441 acatcaccct ttgcagtaaa agaaccctgt tacagcaatg ccgctgtgga aaagcttcca
    55501 ttgcccacac aggacagccg ggacattatt gccgccgcat gcagagcctt aaaccatgtc
    55561 tggcgtgaag ggtaccgcta tatgaaggca ggtgtcatgc tggctgattt cacaccatcg
    55621 ggtatagcgc agccgggatt atttgatgaa atccagcccc gtaaaaacag tgaaaagtta
    55681 atgaaaacac tcgatgaact gaaccagtcg ggaaaaggga aagtgtggtt tgcggggcga
    55741 ggaaccgccc ctgaatggca aatgaaacgg gaaatgttga gtcagtgtta tacaactaaa
    55801 tggcgagata ttcccctagc caggctgggt tagttcagtc atctccgaac atattttcag
    55861 cgtttcttct ggtctggtct tcaccgtcat ttctcgacag actctgctct gtaagaggag
    55921 tactgcaatc cacagacgta tggagaccag attgtttttc cagtaattca agcaatttct
    55981 tttcaaccga aaaagcacct ggtattacgc tggaaatcac cttattaata tcatccagag
    56041 tcgtattttc tgtcagtaac tccgatatca gcaaaggaag acgtttatct atagctgcaa
    56101 acgccgcacc agtaactaac atcgcgcgca tgaaacgtcc ccgctccttc agtttttcat
    56161 ttgcaatgag ctcatctgct atcttatctc ctgtccgttc atccgggaaa agttcaaatg
    56221 agcagcgtct ggatttattt tcatcactca ttagtcacac cacctttcat ttttatcatt
    56281 cccatgacta aatcaaactg cggggatgag ctcagataaa agcgctcatc aggaacctta
    56341 gtcaggttct tcacagcctc ggccacaatt tcagccccac caccaacaca cattacatgt
    56401 gtatagtcgg tgaaacggct gatgacatca ttaactcgtt taataagcaa cttctgccgt
    56461 tcattgataa cattaatgat agactcacgc tgttcagcat tataaatgcg acgggaaagg
    56521 tagtcaggtt cattacggtg cacaatgata ttgtcagcct ggaatgagct cactcttgta
    56581 ttagcatgta cagccatctg ttccttaacc ccggatgtaa tcagggacac gccgatattc
    56641 ggatcacacc aggtttttgt aatccctgtc attttgctac gaacatgcga cacatcaaga
    56701 gttgtaccac cgagatctac gatcaaaagg gattcatcat cctcaagccc cgcaagaaca
    56761 ctgaaaccag cgggaatact ttcaggaaga acggaaacac tgcgaataac aaatgcatca
    56821 ctcccctgca cccgtacttc acgcatcaca ttttttttct ttctttcaat gttttgctta
    56881 tttttctgat tgttggcgtc aaggtattca gagatcggca atgtgacgat gacatcaaca
    56941 ggctgcgctt tcaggccggt ctgctgtagc gcatgctgaa tggccacaac attcacatca
    57001 ctgtactgat agcgtgtttc agtcgttacg acagcatcag cacttaatgg atcaaatgaa
    57061 aacttctcgc cgtcgatctc gtaattagcc ggagcagcat catccagtaa actaaaagac
    57121 cattccggtt taaagctgtt agggctgatt aacgttttca cgtcaccatc ctccaaccag
    57181 gcaagtttaa tgttggtaga accatcatca ataaagattt tcataaccag tctccgttgc
    57241 tcataaacaa atagaaaaaa caaacatact tcatacatgc aatgtatatt caattataat
    57301 gaaaaaacgg tctttttgaa aggacaaatg caacataaaa acacgataaa ttgcatgtac
    57361 attgtacaca caactttaag accagaggaa atcatagata caaaaacatt atgaaggtta
    57421 tatgtgatac ataaatgcaa tataaaaaca tcgtggacat catggttcag gttaagggaa
    57481 aaggaatgaa atggtgaaac tgagaggaaa ggaaagcggt gacgaatcat gtcaccataa
    57541 acagaaagat gtctaatggg gactctttag gtacgtgaag aatccccata acgcatttaa
    57601 aagacacaca tcagataccc ttaaggtatc cgatagttta ttgacctatt gcaccatcac
    57661 gttcaagctt ctctcttata gcctcaataa tatatgagct aaaatccaga gatgttttac
    57721 tggtcgcttt aagttccgca tgagcttcaa aatacgacag cggtatggcc ttaagattct
    57781 tagccttcgt cctggcagcc acgggacgac ctgaaacttc ctctgcacct tcatttggct
    57841 gagacggggc tgttgctgtt acggcagaag aagaagtttg taatctggca gaatccacca
    57901 ggtttttcag tttttcagcc tgtgcactaa atttcgcttt gctcatgaga taacccagga
    57961 tgtttgttag atacttaaaa gatatccatt aaaagcccaa aggatactat atggataccc
    58021 aatagacaca ccttactgtt tgttattttc aatcagatga ttgatttctt cgatcagggc
    58081 aataacttcc ttacctcctg ccgaatagcg cccatgaaca gactcagtga ttcccatccc
    58141 agtctcaatg acatcctcaa attctgcacg agcagaaatg cggctcttca tcagcttcag
    58201 atgtttgaac gatggcagga ttgcatcaag cttagggaat ttcttcttgt taggattaac
    58261 tttacagaga tacaggtgag cagttatatc ggtccccatg atagagctga tttcgtccag
    58321 tactccatca aaatgcatca aaccaatacg ttcagtcagg gagtcttttg aaggaacaat
    58381 aacgcagtct gcaaaggcaa tcgcagtacg ggtaagatct gagtcaaatc ccccacagtc
    58441 aataagtaac gtcttatcgc tgttcttgta gggagtcata atttcgatta actcatccac
    58501 tgttttgggt actttcacgg aaatttcttt tccttcaggt ctcagcccca gaatgataga
    58561 aagcccggta tgagtatcca gatcaactgt atcagacttc atttcaccaa caatgtttac
    58621 ggatgaagtt gtcttaccca caccaccttt gggatgcgta ttagcaataa caggcatagt
    58681 gtatctccag aatccacatg taatacccat aaagacccca taagacacct ttcggatatc
    58741 caagaggtac tcaatagatg tttttttact ctataccggt cctgcatgtc aacagaatat
    58801 gaaataccga taatatatcc atgagatatc cgaaagggat ttaaaaaagt cagagcttat
    58861 ggaattcttt tgagcataga caccgcatcg gactcaggca tcgaaaactg cacccggtgc
    58921 cgtgcagcca catccagtgc aaacaccttc gtgtagacct ccgttgagct gatggactta
    58981 tgccccatca gactctgcag gaccttcagc ggtataccgg catacagcat gtgcatcgca
    59041 taggaatggc ggaacgtatg tggcgtgacc gggacagaga acgtcacacc atcagcggca
    59101 gcggcggcaa ccgcttcccc aatccaggtc ctgaccgttc tgtccgtcac ttcccagatc
    59161 cgcgctttct ctgtcctgcc tgttcgttta ttacgccgtt ccatggggat tttcagtgtg
    59221 gccaccatcg tctgcagctg gctgacgtac caggagtcgg agagcggaac cagccggtga
    59281 gtctgctgcc cggcgggcgt tcttcctgcc gtcctggcgg ctttttcggt ccgttgtttc
    59341 agggtcgcaa gctgcacaaa cggatacgga ggcgtgagcg aaaaatcccc ccgcgtcagc
    59401 gccagtgctt cattaatgcg cgcaccggtg ttccacagtg tggccagcag catcttgcgg
    59461 gccgtttacc ggacgccggc agggcgtggc cacacatctc ttcgtctcgg tactctgagg
    59521 gggcaggggg caaaaaaagt aaaaatgtat tcgccaggtt gcccggaggt gaaggaaaat
    59581 agacatacag cagaacgacg gatagcactt tttgccaaat ggacatcagt attactatgc
    59641 tatagttgct ttaatggata agtgcgcctt gacaaaggcg gtgatttctg ttaacattac
    59701 tctcatagta ttgttccgtc ccgctccacc ccaacaagat ccgtttattt cccgccagac
    59761 tggttatcac cattcaggcc cggatttttt tggatttttt tccggggagc ccccggaacg
    59821 agcttaaaat cggtatgaca aacaggagga tgcgaatgaa cacatcataa cagagctgaa
    59881 agataaaaca ttctgtacgg cattaacagc gttcacgtgt gtgaggcgcc gggtgccttt
    59941 tgacttaaaa acgaggttat tgagatgacc aaaatcaaga cagttacttt tgtaaatact
    60001 tacccgggag ggttctatga aaaacttgtt agacaccgag ggaacggttc tattcccatt
    60061 ccagactgaa atccatttta tttggacgat tttctccacc gttaaacgcc tggttatcgg
    60121 aaccagggac catatttgcc agaagcaata ctggagcgcc tgtctctgta ttttgcttct
    60181 tatggcctat gtgggtctct gtgctgcggt ggtctggttt gtagtgccct ggctgaaggc
    60241 ctttatagtg tcgaaatttg cggtttcggc actatgggtc acgccagtaa agcgcggact
    60301 actctggggt atcggtaaag tggttaccgc cacttgccga agatttactc tgctaaagta
    60361 agtagccgca acgctacacg aactgatggt gaatgtcaac agatactcac catctcctta
    60421 cggcggtggt ccctgtgacc actgccgctt tcgcgtgggt gcaacacggc aaaactcctc
    60481 tgtacaacag gggctcccgc cgtcattttc cggcacaggt gaggccggaa ttcggactaa
    60541 aacgtaaacc gcgggccagt ccggtagcgt tcactatcgg ccagcattct ctcaaccaga
    60601 gagaaatcct tttcaccgca gaacacgtac tgctccgcga actccacctg cagggaattg
    60661 taatgcgtga catgctccgg ggtaagcggc ataagaagat agtccgtata cctccggata
    60721 tgttccagtc tttcaaaagg ccgaatgtga cgggggataa gatgcggcgc cctggcgagc
    60781 agatgttgaa gatgctgctt ttgacgaacc atctgttcac ggatggaggg gcaatacatg
    60841 gccagcatca gcgtcgatga cagggggagg taaatctgta tcccgcgaac ggccagacca
    60901 agattaccgt acggaccgaa gtcattgctg ttcttaagca ctacgggatt gtctgatacg
    60961 taaaacggac gttctggcct ggtttccaga aggtaccagt cctttttcag caggagatca
    61021 atatgtgaca cctgctggac gagctgccgc agaaaaatat ccttggtatc gcccccggac
    61081 gacaggccca gggtttccat ggcctgctct ttagtcgagc cgatggcctc catcttatcg
    61141 gtaagcaccg aaatcatgcc tgagatacgc tccagttcac cgaaggttcg tgcgcgctgc
    61201 acggcaacaa acacggccag cctgtagcgt tcatcatctg tcagcctgcg aatatccctg
    61261 tgcgccagca gggctgcaat taccggtgcc gcgtggtgct cgtaaatccc cagcagtggt
    61321 tcaagactca gacggtccgg gtgattatcg aggttgtaga acgtgttgcg gacggtcgcg
    61381 tcatccggcg tggtggcata ggcgcgcccg gcagccttgt caaaggcgta taagcgctgc
    61441 cgcttaccct ttcccggcgt actgaaatgc ttcagtaaaa aacggggaac cgtatgctgc
    61501 ctgaccacat cggtctgcag attgaatgga tcggccataa ctttatctcc ctgaaaagcc
    61561 acgcaagcgc atcggtgcat ctgtattgac ggcatgggca gctggtttgc agcttagcat
    61621 tagaaaacag aaccgttcga taatacctgt gtttaacgcc ctgtcagtac cgatggaatt
    61681 taaatctccg gaaccgccgg aaaccggatc tgcgcggcag aaaagacgca aatgatatga
    61741 taataaaaaa tacacataaa atcatatcag atatgatttt catcattgat gataaaacca
    61801 tatctgatat tatcagaaat caggagcaca caaatcattc aggatatgat tattacctgt
    61861 cagaatatta tcagatggag cgtgcaggat ggatcaggaa atgacgtttt cactcagcta
    61921 tgaacaactg acccggtttg cggaacgacg catccgggaa tgcaatctgg acagccaggg
    61981 cgcgatttat ctctgcgagt cagccaaagc cggagccgtg ctgatcttct ggcatgaact
    62041 ggccataaat ggctatgcct caatgaatgc cataaagaga caggagctca ttgacgccga
    62101 ctttcagcgc ctcagaaacc tcatctggcc ggaggatgac aggtaatgac ccagagccgc
    62161 cgaccgtcgc cactgcagcg gagagtgctg attgtgctgg ctgcccttga tgaaaaacgg
    62221 ccaggacccg tgctgacgcg ggacatagaa cgggtgctgg agcgaagcgg ggaagccccg
    62281 gtctacgggc caaacctgcg cgcctcctgc cgtcgtctgg aggatgccgg ctggctgcgt
    62341 accctgcgcg ctccgaacct gcagctggca gtagagctga cggacgccgg ccgggccgtg
    62401 gcacagcctc tgctgctggc agaacaggac cgcctccggg cagaacagcg tgcggcagaa
    62461 gtcgtcgtac tgccgctggt cccggcagca ggtctgcccg cagatggcac ttctgccact
    62521 gatctggcgg tgcaactgaa cggcataacg tatcaggcct gccgtgggga ttttgtggtg
    62581 cgtctagacg gcagcacctg tctgcagtta tggaataaag aaggc
//
LOCUS       DAESHI010000010        59896 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.010, whole genome shotgun sequence.
ACCESSION   DAESHI010000010 DAESHI010000000
VERSION     DAESHI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59896
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.010"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..1474)
                     /gene="ggt"
                     /locus_tag="KW011_000653"
     CDS             complement(<1..1474)
                     /gene="ggt"
                     /locus_tag="KW011_000653"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBH7659078.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGMGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTV"
     gene            1594..2034
                     /locus_tag="KW011_000654"
     CDS             1594..2034
                     /locus_tag="KW011_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HBH7659079.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(2021..2764)
                     /gene="ugpQ"
                     /locus_tag="KW011_000655"
     CDS             complement(2021..2764)
                     /gene="ugpQ"
                     /locus_tag="KW011_000655"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBH7659080.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(2761..3831)
                     /gene="ugpC"
                     /locus_tag="KW011_000656"
     CDS             complement(2761..3831)
                     /gene="ugpC"
                     /locus_tag="KW011_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HBH7659081.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            complement(3833..4678)
                     /gene="ugpE"
                     /locus_tag="KW011_000657"
     CDS             complement(3833..4678)
                     /gene="ugpE"
                     /locus_tag="KW011_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HBH7659082.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(4675..5562)
                     /gene="ugpA"
                     /locus_tag="KW011_000658"
     CDS             complement(4675..5562)
                     /gene="ugpA"
                     /locus_tag="KW011_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HBH7659083.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(5660..6724)
                     /gene="ugpB"
                     /locus_tag="KW011_000659"
                     /pseudo
     CDS             complement(5660..6724)
                     /gene="ugpB"
                     /locus_tag="KW011_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            complement(7008..7721)
                     /gene="livF"
                     /locus_tag="KW011_000660"
     CDS             complement(7008..7721)
                     /gene="livF"
                     /locus_tag="KW011_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HBH7659084.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(7723..8490)
                     /gene="livG"
                     /locus_tag="KW011_000661"
     CDS             complement(7723..8490)
                     /gene="livG"
                     /locus_tag="KW011_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HBH7659085.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(8487..9764)
                     /gene="livM"
                     /locus_tag="KW011_000662"
     CDS             complement(8487..9764)
                     /gene="livM"
                     /locus_tag="KW011_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HBH7659086.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(9761..10687)
                     /gene="livH"
                     /locus_tag="KW011_000663"
     CDS             complement(9761..10687)
                     /gene="livH"
                     /locus_tag="KW011_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HBH7659087.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(10735..11844)
                     /gene="livK"
                     /locus_tag="KW011_000664"
     CDS             complement(10735..11844)
                     /gene="livK"
                     /locus_tag="KW011_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HBH7659088.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            12268..12651
                     /gene="panM"
                     /locus_tag="KW011_000665"
     CDS             12268..12651
                     /gene="panM"
                     /locus_tag="KW011_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HBH7659089.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWIADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(12850..13953)
                     /gene="livJ"
                     /locus_tag="KW011_000666"
     CDS             complement(12850..13953)
                     /gene="livJ"
                     /locus_tag="KW011_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HBH7659090.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(14224..15078)
                     /gene="rpoH"
                     /locus_tag="KW011_000667"
     CDS             complement(14224..15078)
                     /gene="rpoH"
                     /locus_tag="KW011_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HBH7659091.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(15323..16381)
                     /gene="ftsX"
                     /locus_tag="KW011_000668"
     CDS             complement(15323..16381)
                     /gene="ftsX"
                     /locus_tag="KW011_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HBH7659092.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(16374..17042)
                     /gene="ftsE"
                     /locus_tag="KW011_000669"
     CDS             complement(16374..17042)
                     /gene="ftsE"
                     /locus_tag="KW011_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBH7659093.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            complement(17045..18541)
                     /gene="ftsY"
                     /locus_tag="KW011_000670"
     CDS             complement(17045..18541)
                     /gene="ftsY"
                     /locus_tag="KW011_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBH7659094.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            18691..19287
                     /gene="rsmD"
                     /locus_tag="KW011_000671"
     CDS             18691..19287
                     /gene="rsmD"
                     /locus_tag="KW011_000671"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HBH7659095.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            19277..19546
                     /locus_tag="KW011_000672"
     CDS             19277..19546
                     /locus_tag="KW011_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HBH7659096.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(19549..19908)
                     /locus_tag="KW011_000673"
     CDS             complement(19549..19908)
                     /locus_tag="KW011_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HBH7659097.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            20049..20675
                     /locus_tag="KW011_000674"
     CDS             20049..20675
                     /locus_tag="KW011_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HBH7659098.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            20749..22947
                     /gene="zntA"
                     /locus_tag="KW011_000675"
     CDS             20749..22947
                     /gene="zntA"
                     /locus_tag="KW011_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HBH7659099.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(23049..23294)
                     /gene="tusA"
                     /locus_tag="KW011_000676"
     CDS             complement(23049..23294)
                     /gene="tusA"
                     /locus_tag="KW011_000676"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HBH7659100.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            23515..24180
                     /locus_tag="KW011_000677"
     CDS             23515..24180
                     /locus_tag="KW011_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HBH7659101.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            24253..24810
                     /gene="dcrB"
                     /locus_tag="KW011_000678"
     CDS             24253..24810
                     /gene="dcrB"
                     /locus_tag="KW011_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HBH7659102.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(24814..26031)
                     /locus_tag="KW011_000679"
     CDS             complement(24814..26031)
                     /locus_tag="KW011_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH7659103.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            26163..27212
                     /locus_tag="KW011_000680"
     CDS             26163..27212
                     /locus_tag="KW011_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBH7659104.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            27267..27854
                     /gene="acpT"
                     /locus_tag="KW011_000681"
     CDS             27267..27854
                     /gene="acpT"
                     /locus_tag="KW011_000681"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HBH7659105.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            27965..29539
                     /gene="nikA"
                     /locus_tag="KW011_000682"
     CDS             27965..29539
                     /gene="nikA"
                     /locus_tag="KW011_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HBH7659106.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            29539..30483
                     /gene="nikB"
                     /locus_tag="KW011_000683"
     CDS             29539..30483
                     /gene="nikB"
                     /locus_tag="KW011_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HBH7659107.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            30480..31313
                     /gene="nikC"
                     /locus_tag="KW011_000684"
     CDS             30480..31313
                     /gene="nikC"
                     /locus_tag="KW011_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HBH7659108.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            31313..32077
                     /gene="nikD"
                     /locus_tag="KW011_000685"
     CDS             31313..32077
                     /gene="nikD"
                     /locus_tag="KW011_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HBH7659109.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            32074..32880
                     /gene="nikE"
                     /locus_tag="KW011_000686"
     CDS             32074..32880
                     /gene="nikE"
                     /locus_tag="KW011_000686"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HBH7659110.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            32886..33287
                     /gene="nikR"
                     /locus_tag="KW011_000687"
     CDS             32886..33287
                     /gene="nikR"
                     /locus_tag="KW011_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HBH7659111.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(33407..33766)
                     /locus_tag="KW011_000688"
     CDS             complement(33407..33766)
                     /locus_tag="KW011_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBH7659112.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(33763..34036)
                     /locus_tag="KW011_000689"
                     /pseudo
     CDS             complement(33763..34036)
                     /locus_tag="KW011_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            complement(34109..35233)
                     /locus_tag="KW011_000690"
     CDS             complement(34109..35233)
                     /locus_tag="KW011_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH7659113.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(35233..37968)
                     /gene="rbbA"
                     /locus_tag="KW011_000691"
     CDS             complement(35233..37968)
                     /gene="rbbA"
                     /locus_tag="KW011_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HBH7659114.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(37965..39032)
                     /locus_tag="KW011_000692"
     CDS             complement(37965..39032)
                     /locus_tag="KW011_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBH7659115.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(39398..41020)
                     /locus_tag="KW011_000693"
     CDS             complement(39398..41020)
                     /locus_tag="KW011_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBH7659116.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTINDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(41282..42889)
                     /locus_tag="KW011_000694"
     CDS             complement(41282..42889)
                     /locus_tag="KW011_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBH7659117.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKKYRSNTVEVN
                     MLNDFSQIDNDLGLNIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKSDITYKVNGLS
                     IIPENFFTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNAN
                     TAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHENMASSAYIHKNNKLLYN
                     LLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMDG
                     NKDSRINKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCHYDSETKVL
                     TSHHGIIRNEENECYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDRLIHLIRS
                     NAVASTPVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTM
                     GLNSWKIAPNERGDKKGVPGLSCFQPQ"
     gene            43274..44326
                     /locus_tag="KW011_000695"
     CDS             43274..44326
                     /locus_tag="KW011_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HBH7659118.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(44642..45844)
                     /locus_tag="KW011_000696"
     CDS             complement(44642..45844)
                     /locus_tag="KW011_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBH7659119.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            46076..47575
                     /gene="pitA"
                     /locus_tag="KW011_000697"
     CDS             46076..47575
                     /gene="pitA"
                     /locus_tag="KW011_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HBH7659120.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(47819..48154)
                     /gene="uspB"
                     /locus_tag="KW011_000698"
     CDS             complement(47819..48154)
                     /gene="uspB"
                     /locus_tag="KW011_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HBH7659121.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            48546..48980
                     /gene="uspA"
                     /locus_tag="KW011_000699"
     CDS             48546..48980
                     /gene="uspA"
                     /locus_tag="KW011_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HBH7659122.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            49298..50767
                     /gene="dtpB"
                     /locus_tag="KW011_000700"
     CDS             49298..50767
                     /gene="dtpB"
                     /locus_tag="KW011_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HBH7659123.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(50816..51568)
                     /gene="rsmJ"
                     /locus_tag="KW011_000701"
     CDS             complement(50816..51568)
                     /gene="rsmJ"
                     /locus_tag="KW011_000701"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HBH7659124.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(51576..53618)
                     /gene="prlC"
                     /locus_tag="KW011_000702"
     CDS             complement(51576..53618)
                     /gene="prlC"
                     /locus_tag="KW011_000702"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HBH7659125.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            53821..54663
                     /gene="rlmJ"
                     /locus_tag="KW011_000703"
     CDS             53821..54663
                     /gene="rlmJ"
                     /locus_tag="KW011_000703"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HBH7659126.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            54735..56087
                     /gene="gorA"
                     /locus_tag="KW011_000704"
     CDS             54735..56087
                     /gene="gorA"
                     /locus_tag="KW011_000704"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBH7659127.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(56141..56224)
                     /locus_tag="KW011_000705"
     CDS             complement(56141..56224)
                     /locus_tag="KW011_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HBH7659128.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            56252..56425
                     /locus_tag="KW011_000706"
     CDS             56252..56425
                     /locus_tag="KW011_000706"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659129.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            56964..57317
                     /gene="arsR"
                     /locus_tag="KW011_000707"
     CDS             56964..57317
                     /gene="arsR"
                     /locus_tag="KW011_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HBH7659130.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            57371..58660
                     /gene="arsB"
                     /locus_tag="KW011_000708"
     CDS             57371..58660
                     /gene="arsB"
                     /locus_tag="KW011_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HBH7659131.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            58673..59098
                     /gene="arsC"
                     /locus_tag="KW011_000709"
     CDS             58673..59098
                     /gene="arsC"
                     /locus_tag="KW011_000709"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HBH7659132.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(59232..59528)
                     /locus_tag="KW011_000710"
     CDS             complement(59232..59528)
                     /locus_tag="KW011_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH7659133.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            59635..>59896
                     /locus_tag="KW011_000711"
     CDS             59635..>59896
                     /locus_tag="KW011_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBH7659134.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
BASE COUNT        14010 a        16148 c        15123 g        14615 t
ORIGIN      
        1 gcacggtggt aataatccga ctcccgccag gactaccggt aaccagccaa gttttaccgt
       61 ctttcaccac aatggttggt gacatcgatg acagcggacg tttattcggc ccgacggcgt
      121 tggcatcacc gcccaccagc ccgtaaatgt tcggtacgcc cggtttggcg gagaaatcat
      181 ccatctgatt attaagcaga ataccgctct cgcccgcgac aatgcccata ccgaaggtgg
      241 tgttcagcgt ataggtcacc gccaccgcgt taccgtcttt atccactaca gagtaatggg
      301 tagtttgatt actctcataa ggcgcaagct tgccagggcg aatctcactg gacggcttcg
      361 ctttgttgat atcaatttga tcggcaatgg atttggcata ggctttattg gtcagtgcct
      421 gccacgggac tttgacaaaa tccgggtcgc caagatattc cgagcggtcg gcgtaggcgt
      481 atttctccgc ttctgccatg atttgcatcg catcggcgct gccaaagccg tatttcttca
      541 tatcgaagtt ttccagaata ttgaggattt gtacgatatg gatcccgccg gaggatggcg
      601 gtggcatgga gtaaacctga tacccgcgat aatcgccgct tatcggagtg cgttcgaccg
      661 ctttataggc cgctaaatct tctttagtga tcaagccacc gtttttctgc atctcctggg
      721 cgatctgttc cgcaatcgtg cctttataga attcgtccgg gccgttttca gcaatcatct
      781 ccaggctctt tgccaggttc gcctgcacca gcgtgtcgcc ctttttcagc ggctcgccct
      841 ctttccagaa gatagcttta ctgttttcgt gattcggcaa cacttcgcta ccgtaggttt
      901 tgagatcgtc agccagcgcg tcgttaacga taaaaccatc gcgtgccagt ttaaacgcgg
      961 gctgcacgac tttgttcagc ggcatggtgc cgtatttatc cagcgccagc gagaaacctg
     1021 ctaccgtacc cggtgtgccg gaagccagat gcgaagtgag tgattttttg ctgtccgggt
     1081 tgccctgatc atcgaggaac atatcgcggg tcgctttggc gggtgccatt tcgcggaaat
     1141 cgatagccgt ggtattgcca tttttcgagc ggattaacat aaaaccacca ccgcccagat
     1201 tccctgcctg cggatgcgtt accgccagcg cgtagcccac cgccacggcg gcatcaacgg
     1261 cattcccgcc ctccttgagg atatccaccc ccacctgagt ggcagtggcg tccacagacg
     1321 ctaccattcc ctgtttcgcg cgtaccgggt ggaagacatc ttcctccaca ccatacgaga
     1381 cgggcggcgc aggaggcgcg gcggcggcgc taaaacaact tcctgagagc agagcagcaa
     1441 tggccacccg gcgtaaaaac gtcggtttta tcatcgttat tctccagaga ttaaggggca
     1501 accccagcta agcctggtat ataactctga attaatcatc gttttgtcgg gaagcgagta
     1561 aacttaaagg atatcttcag aggaggatac gcgatgaaac gacttctgat tcttacggca
     1621 ctcctgccgt ttgtcggctt tgcacagccc attaatactc tgaacaaccc taaccagccg
     1681 gggtatcaga tacccagcca gcagcggatg caaacccaga tgcagactca gcaaatccag
     1741 caaaaaggga tgctgaatca gcaactgaaa acgcaaactc agttgcaaca gcagcattta
     1801 gaaaaccaga taaacaataa ttctcagcgg gtgttgcagt cacagccggg ggagcgaaat
     1861 cccgcccggc agcaaatgct gcccaacacc aacggcggga tgttaaacag caaccgtaat
     1921 ccggatagtt cgttgaatca gcagcatatg ctgccggaaa ggagaaacgg cgacatgctg
     1981 aatcagccca gcacgccgca gcctgacatt ccgttgaaaa ctattgggcc gtaaagttcg
     2041 gaccaatcac gtcaatcgca tcggtacaga tgcaatccac gccccagcgc agcaactctg
     2101 ccgcgcgctg gggtttattg acggtataaa ccagaatccg taatccggcg tctttcaact
     2161 gcatcactcg cgctttatcg agtaacttat gattgagatg aatagagacg cagcccagcc
     2221 gcgcggtcag ttcgcgccag tcgtcgcgcc actcatcaag caataaacca cgcggcagtt
     2281 ccggtgccgc ctgttgtgca gcttctaaag catcaatctc aaacgatgac agcagcggcg
     2341 gcgtcatacc ggcccacagt tggcgtgccg ccagcgccac cattttgccc gttaatggtc
     2401 cagtgccagt ggtgggtttg atttcgatat tcgccatcat cccgtgttcg cgacagcgtt
     2461 ccgccacctg cgaaagcaac ggcagcggct cacctttaaa cgctttgctg taccaactgc
     2521 ccgcatccac gcgcagtaaa tcctgccagt tcagttcacc cgcgacgccc cagccgttgc
     2581 tggtgcgttc gagattgtcg tcatggagca ggaagatctc gccatctttc gataacttcg
     2641 cgtcaaattc gatcatctta tgaccgtatt ttgccccgac gtcgattgcc gccagggtgt
     2701 tttccggggc cagcttaccg ccgccacgat gagcgacgat gcggggataa ggccagttac
     2761 tcatactcgt tgtcctgttt caccatcaaa aagatgcagc tgattttccg ccaaatgcag
     2821 ccacagcgtg ctgcctgccg tcgggcgctc ctgatgcgcc agtcgcacca ccagcttctg
     2881 ctcgccccag cgtccgtgcg ccaggttatc tgcgccgagg atctccagcg tgtccatcac
     2941 cagcggcaca ccaccttctg cctgcgagct tagcgctata tgttccgggc gaatgccgag
     3001 agtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca cgataccgcc
     3061 gtccagttcg aaatgcgtgc cttcattatt cacgcggcct gtcagcaggt tcatcgccgg
     3121 gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt caactggtgt
     3181 gccaatctgt tcggcaacgc caccgttcat caccattact cgctgggcga gcgtcatcgc
     3241 ttcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt gcaactgttg
     3301 cagttcaaga cgcatctgca cgcgcagctt ggcatcgagg ttagagagcg gctcatcaaa
     3361 caagaacacc gccggatcgc gcacaatcgc gcggcccatc gccacacgct ggcgctgacc
     3421 gccggatagc tcacgcggac gacgcttaag cagaccgtcc agttccagaa tgcgcgccgc
     3481 ttctttaacg cgctcggcaa tttgctgctt gcccatgccg cgaattttca gcccccacgc
     3541 catgttctcc tctacgctca tatgcggata aagcgcgtaa ttctggaaca ccatcgcaat
     3601 cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga tatcgccttc
     3661 tgtcacccgc tccagcccgg caaccatgcg cagcagcgtc gatttcccgc agccagacgg
     3721 cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt taatgacctg
     3781 ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca tcttatttct
     3841 cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc accaccggag
     3901 ggataagcgt taacaacatc gccgccatta ccgagttcca ttccgtggtg ccttcgcctg
     3961 tagcgatcat ccctttgatc cctgccacgg tggtgccgag atcaacgtcg gtaataatca
     4021 acaacggcca caaatactga ttccagccgt agataaaggt gatcacaaac agcgccgcca
     4081 gattagtttt ggacaacgga aaaacgatgt cgcaaaagaa acgcattggt gacgcgccgt
     4141 cgatccgcgc ggcttccacc aattcatccg gtagggtcat aaagaactgg cggaacagaa
     4201 acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg tcgagcattt
     4261 tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc agcatcaggg
     4321 tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag acaatggcaa
     4381 atgccgagag catcgagacg gtaattttgc cgagcgtaat gctgaacgcc atcacaaagc
     4441 tgttaagcaa catccgccag aacggcgcgc tattcgtgcc taccccgtta acccagatgt
     4501 tgtggatatt ttccagcaga tgtgtgccgg ggatgagcgt catcggcgcg gcatagacgg
     4561 cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg atcaccgcga
     4621 tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc tcaatcattg
     4681 gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga tgacgaggaa
     4741 catcaacacc accgactgcg cggcagacga agccagatcc agtccggtaa agccttcgcg
     4801 gtagatctta taaatcagcg tagtggtggc ctgaaccggc ccgccggacg tggcggcgtc
     4861 gatcaccggg aaggtgtcga agaaggcgta caccaggttc accaccagca ggaagaaact
     4921 caccggagcg ataagcggca gagcaatctt aaagaagcgg cgaatcggcc ctgcgccgtc
     4981 aatagctgcg gcttcaatca acgaacgggg aatggagtgc agcgcggcat agaagaacag
     5041 aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca ttgcctgacc
     5101 gctgttttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg tgatcagccc
     5161 gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag ccacggcata
     5221 cggcagcagc atcaaggttt gatagaaacg gctgccgcgc acgatgtact ccaccagcgc
     5281 cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga attttatcgt
     5341 cgtccagaag gagtctagat agtagctgtc atggaacagc gtgacgaagt tatccagacc
     5401 gacaaactgg ctggagaaac caaacggatc gacgctttgc cgcgagtacc acaacgcttc
     5461 gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga ccagcagata
     5521 aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta attcctgaac
     5581 tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga taagcgaaac
     5641 gcatccgaca ttacactgat taagacttcg tcgatttctc aaagcggcgc agcaactgat
     5701 ttccacgctc aacggcggta tccagtgcct gctgtggtgt cttcttaccg gtccacacgc
     5761 tctccagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga cgcagccctt
     5821 tggtgaacgg caacggcggc ttattcagca tctgacgcgt cgcggtatcc gcccctgggt
     5881 ttttctcata aaagccctgc tcacgggtca ggtcatacgc tgctttggtg attggcagat
     5941 aaccggtttt ctgatgccac tcggcagcgt tttctggctt cgcgaggaag tcgaggaact
     6001 tcgccacgcc ggtatacgtt tctttatttt tgccctgcat cacccacagg ctggccccgc
     6061 cgataatggc gttttgcggt gcatctttcg catcggcgtc gtaaggcatc atgcctacgc
     6121 catagttaaa tttggcgtac tcacgaatat tagcgagaga accggaagag gcggtggtca
     6181 tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg acgtagctga
     6241 agtcgccctt cttgttcatc tcttcgagca tggcaatgtg tttcacctgc tccggcttgt
     6301 tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca aacggcagac
     6361 cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg gcgtagccgc
     6421 acttcatacc ggaggctttc agttttgcgg aagagtccgc cagatcctgc caggttttcg
     6481 gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa tagagaacgg
     6541 gagtcgagct gttgaatggc tgggagagta agtggcccgt tttgctgtcg gagtagtaac
     6601 cggaaaccgt cggcacaaac tgcgactcat cgaattgaat ccctgcctct ttaaacacgt
     6661 catataccgg tttaatggct ttcgatgcca tcatggtggc ggtgccaact tcataaacct
     6721 gcaagtaagg tgtcagaaag atgacaaggc ggtgacggcg tgggtgaggg aaaatgggag
     6781 atggggcacg gataagcgga aaaatagaag gtctgaatca aactctacag attactcatc
     6841 gtttcatgcc ggatgcggcg taaacgcctt atccggccta caagatcgtg caaattcaat
     6901 atattgcaac tcacccagta ggcctgataa gcgcagcgca tcaggcaatt ttacatttgt
     6961 cacctgtctc aaaggagtct tttgactcct tatcaatcaa cgtgttatta cccgcctaaa
     7021 tacgcacttc tcaccgcttc attcgccagt agtgcatcac cagtatcgga gagcaccaca
     7081 tggccgtttt ccagcacgta gccgcgatcc gccagcttta gcgcctggtt ggcgttctgc
     7141 tcgacgagga agatagtcat cccctgctcg cgcagctgct cgatggtgtc gaaaatttgc
     7201 tggatgataa tcggcgcaag accaagcgat ggctcatcaa gtagcagcaa acgcgggttg
     7261 ctcatcagcg cgcgaccaat cgccagcatt tgttgctcac cgccggacat ggtgcctgcc
     7321 cgctgaacac ggcgctcatg cagacgtggg aacagttcat acacccactt tatgcgctcc
     7381 tggaactggt cgcgttcggc aaaaaagccg cccatcgcca ggttctcttc caccgtcatc
     7441 cgcgagaaga cgcgacgccc ttccgggaca atcgctaccg cttcgcgcat gattttcgcc
     7501 gtctgccagt cggtaatgtc tttatcatca aacacaattc gcccgctggt agcgcgcgga
     7561 tcgccgcata acgtgccgag caaggtggtt ttccccgccc cgttcgcgcc aatgagggtg
     7621 acaatctcgc cctgattgat atgcaggctc acctcatgca gcgcctggat tttgccgtag
     7681 tgggcgctga ctttgtcaaa ggacaacatg actttttcca tcttatgcct cacctaaata
     7741 ggcacggatc acgtccgggt tattacggat ttgctccggc gtaccgtttg ccagtggcgt
     7801 cccctgattg accacataaa ttcggtctga aatccccatc accagcttca tatcgtgttc
     7861 aatcaacaag atagtggtgt tgtgatgatt gcgcagttcg gcaatcagct catccagctc
     7921 tttggtttct tttgggttaa gacctgccgc cggttcgtcg agcattaaaa tctccggctg
     7981 cgtcaccatg cagcgggcaa tctccagacg gcgttggtca ccataggcca ggttactcgc
     8041 ctgacggttg gcgtgttcca gcaaaccaat gcgctcaagc caggtggcgg cgcggtcgag
     8101 cgcttcgctc tgggcgcgac ggaaagaagg cgttttcaac aggccagaga acagcccggt
     8161 tttcagttgt tgatgctgcg ccaccagcag gttttcaatt accgtcattt cacggaacag
     8221 acgcacatgc tggaaggtgc gcaccacgcc catgcgggca atttgctgcc ccggtaaacc
     8281 ttccaggtgc tgattgcgca gtaaaatggt gccgccggtg ggtttgtaga atccggtcag
     8341 acagttaaaa accgtagttt ttccggcacc gttagggccg attaacgaga cgatctcctg
     8401 cgggtacagt tcaagattga cgttgttcac cgccagcagg ccgccgaagc gcatcatcag
     8461 gccgttcaca gataataatg gctgactcat gcctgctctc ctttcgctgc gccgtttttc
     8521 agcttcagtt gcgggcgcgt catgggcagc aagccctgcg gacgccagat catcatcagc
     8581 accatcaaac caccgagcat taacatgctg tattcgttga aatcacgcat caattcgcgc
     8641 gataccacca gcaaaattgc cgccagaatc accgcaaact gcgatcccat accgccgagc
     8701 accactatcg ccagtacaaa cgccgattca gcaaaggtga aggattccgg gctgacaaag
     8761 ccctggcgcg cagcaaacag cgttccggca aaaccggcaa acgcggcact gatggtaaag
     8821 gcagtcagct tgatacgacg cgggcttaag cccagcgaac ggcaggcgat ctcatcttca
     8881 cgcaacgctt cccacgcacg ccccagcggc atccgcagca ggcggttaat gacaaacagg
     8941 cttagcacca ccagcagcaa cgccaccaga tacaggaaga tgacgcgatc ggagggatcg
     9001 tatttcaggc caaagaaatt actgaacgtg tcccaaccgc cttcacgggc ggtacggctg
     9061 aactcaaggc cgaagaaagt cggtttcggg atctgactga taccgttcgg gccgccggta
     9121 atttcggtgt tattgagcag caatatgcgc acaatttcgc cgaaaccgag ggtaacgatc
     9181 gccagataat caccgcgcag acgcaacacc ggaaaaccaa gcaggaagcc cgccgccgct
     9241 gccatcagtc cggcaatcgg caaacaggtc cagaacccca gaccgtaata gtgattgagc
     9301 agcgcaaaag tgtaagcgcc gatggcgtaa aaaccgccgt accccagcac cagcagacca
     9361 gaaagaccaa caaccacatt cagcccgaga ccgaggataa tgtagatcat ggtcagagtg
     9421 gcgatatcca ccgtcccacg tgacaccata aacggccacg acaccgcaag caccagcagc
     9481 gccacgagga acagtttctg cttcaccgtg gagccatcaa tcgcgggcag aataaacttc
     9541 ggcccggaaa cgctttttag ccctttctgg aaagcaggtc gcaaaagctg gaagaaaaag
     9601 accaccgccg tgccgataaa cacccactgc caacggacat ccgaagcgct gtcgaccacc
     9661 agtttggtgc catccagctc cagttgcacg cccataaaga cgccagcgag gacaaagaac
     9721 atcgcggcag agagcagtgc cattgcaata tgcatcggtt tcatactttc tctacctccg
     9781 ggcgacccag aataccggtc ggcatcacca gcagcaccag aatcagcagg gcgaatgaga
     9841 ccacatcttt atattccgta ctcagatagg cagaagagag cgcctccgca atccccagga
     9901 tcagcccgcc aatcatcgcc cctggaatgc tgccaatccc accgagcacc gcagcggtaa
     9961 aggctttcat cccggccata aagccgatat aggggttaat gacgccgtag aactgaccga
    10021 gcagtacgcc agccactgcc gccatcgccg cgccaataac aaaggtcaaa gcaatcaccc
    10081 ggtcggtgtt gatgccaagc aggctcgcca ttttcagatc ttccgcgcag gcacggcagg
    10141 cgcgccccat acgggaataa cgaatgaaaa tcgtcagcgc cagcatggcg aggaaagtaa
    10201 caatccagat cactgcctgc atggtggtaa tagaggcaga gaagttttcg ctatgcccca
    10261 ccacccactg accgttaaac agactgggca gcgccacgtc acgcgaacct tcggtcaggc
    10321 taacgtagtt ttgcaggaag atggacatgc cgattgccga gatgagcgca atcaggcgct
    10381 tagagttacg caccgggcga taggctaccc gttcgatact ccagccgtag gcgctggcaa
    10441 tgacgattgc gccgacgaat cccgccgcca ccagcagcca gccggtatca atgcccatca
    10501 tcatcagtgc ggcgatgatc ataaaggaga catagctacc aatcatgtaa acctcgccgt
    10561 gggcgaagtt gatcatgccg ataatgccgt aaaccatggt gtagccgatg gctatcagcg
    10621 cgtaggtact gcccagcgtg acgccgttaa acatctgctg caagaaatat aaaaactgct
    10681 cagacataag gtaacctttc taaacccgcc cgcatttcac gggcggtggg atgatcactt
    10741 ggctgccgtg gatgaaccat cggcgtgcca ctggaagaca ccaaaatcaa atcccttaag
    10801 atcgcctttt tcatcccagt tcagcggccc aatcacggtg tttgcaccgt tagcttttaa
    10861 atctttcacc agcgccagcg gctcatcgct gccagtacgc tcaagggcag tcgccagaga
    10921 ttgcaccgcc gcgtaggtga tccagacata aggcccggac ggatctttct tgtctgcttt
    10981 cagcgcatca acgatgccct ggtttgccgg atcctggtca tagcgttttg gcatagtgac
    11041 caacatgcct tcggcagcat cgccagcaat attcgacaat gatgcgttac ccacaccttc
    11101 cggccccata aactgagttt tcaggccaac ggaacgggcc tggcgcagca tctgccccat
    11161 ttccgggtag taaccgccgt agtaaacgaa gtcgatgttt tcttttttca ggcgggcgat
    11221 cagcgcggag aaatctttct ccccggcggt aataccgtcg aagaagacga cgttggcgtt
    11281 agccgctttc agcccgtcct gcaccgaacg cgccagccct tcgccatact gttgtttgtc
    11341 gtgaatgatg gcgatgcgct ggggcttcac cgtctcaaga atgtattttg ccgccgttgg
    11401 cccctgggaa gagtccagcc cggcagtacg cataatgtgt tgataaccgc gttgggtcag
    11461 ctccgggttg gtcgctcccg gcgagatcat cagaatacct tcgtcttcgt agatatctga
    11521 tgcaggttgg gtagaagaag aacacagatg accaataacg tatttaatgc cgtcattaac
    11581 gattttgttg gcgaccgcaa cggcttgttt cgggtcgcag gcgtcgtcat attccacgcc
    11641 aaccagcttg tcgcccttaa ttcccccttt ggcattaatg tctttaatcg cctgacgcgc
    11701 gccgttaaat tccatatcgc cccactgggc aatcgggccg gacatcgcgc cgacaacggc
    11761 gactttaata tcgtcagcca tagcggtgtg tgaaattgcc agtgcaatca tccctgcgat
    11821 gatagttttc gcattccgtt tcatagtcaa aaatccccat tcgtgatgtt gtgttgcttt
    11881 gtttttatgt gttaacaaat cagactgttc tttttttata ctgcactgtt tttgcctgtc
    11941 tgattaaggg tttagcgcag tattttgtga taacagtgat taaaatccct atttttcagt
    12001 cgattaagaa cagataatat tctgaattta ttgatagata aacagaaaaa agtgcctttg
    12061 tcagcataaa ataacggcac aaagggcgga ataattcact accattcagg ggattatgct
    12121 gggtattttt cattctctaa tgttttaact ttgtaattat tgctgttaaa aaattaatca
    12181 cctgccaaaa gaaataaaaa agagaaagcc tccgattaaa ttatttcgct acactggttc
    12241 cacttttgtg atttacacgg gttacccatg aagctgacca tcattcgatt agaaaacttt
    12301 agcgaccagg accggattga cctgcaaaag atctggccgg agtattcccc ttcctcttta
    12361 caggttgacg ataaccaccg tatctacgcc gcgcgtttta acgagcgcct gctcgctgcc
    12421 gtacgggtaa ccttaagcgg caccgaggga gcactggatt ccctgcgtgt gcgggaagtc
    12481 acccgacgtc gcggtgtggg gcaatatctg ctggaagagg ttttgcgtaa caatcctggc
    12541 gtttcatgct ggtggatagc ggatgcaggc gtggaagatc gcggtgtgat gacagcgttt
    12601 atgcaggcac tgggatttac ggcgcaacag ggcggctggg agaagcgtta atcgtcaagt
    12661 tgatttcaaa agtgatgacc aacgagaaat tgcctgatgc gctacgctta tcaggcctac
    12721 aatgtgtgtt gcaatttact gatttctttg gatcttatag gccggataag gcgtttacgc
    12781 cgcatccggc atgaagcaac gtactcgata ttagcaattt ggcggcaacc caaagttgcc
    12841 gattaatgat tacttcgcat ctgtcgccgt gccgttggcg tgccagtcaa atacgccgaa
    12901 ctcaaagcct ttcagatcgc ctttctcatc ccacgtcagc ggtcccatta cggtatccac
    12961 ggagttcgct ttcaggtatt tggcgatttc agccggatcg tcagactgat tcaggcccgc
    13021 ctgcaaagat tgcagcgcgg cgtaggtggt ccaaacgaat gcgccgcttg ggtcctgttt
    13081 tttcgctttg atcgcgtcaa caatgggttt gtttgccgga acctgatcgt agttcttcgg
    13141 cttggtcacc agcagccctt ccgctgattc gcccgcaatg ttagacagcg aaacgttagc
    13201 cacaccttcc ggccccataa actgagtttt cagccctgcc gcgcgtgcct gacgcaggat
    13261 ttgccccatt tccgggtggt aaccgccgta gtaaacgaag tcgatattct ctttcttcag
    13321 acgcgccacc agcgtagaga aatctttttc cccggcggtg ataccatcaa agaacaccac
    13381 gttagcattg cctttcttca ggccgtcctg caccgctcgt gccagacctt cgccgtattg
    13441 ctgtttgtcg tgaacgatag caatacgctg cggtttcact ttctcaagaa tatatttcgc
    13501 cgccgtcggc ccctggtcgg agtccaggcc ggtggtgcgc aggatcagct gatagccacg
    13561 ggcggtcagc tccggcgcgg ttgccgctgg ggtgatcatt aaaatgcctt cgtcttcgta
    13621 gatgtcagac gcaggctgcg ttgatgaaga acagaggtga ccaatcacat atttaatgcc
    13681 gtcgttaacg actttgttcg ccaccgcaac cgcctgtttc gggtcacagg catcgtcata
    13741 ttttacgatt tgcagtttgt tgcctttaat gccgccttta gcgttgatat ccgcaaccgc
    13801 ctgctctgcg ccggtaaact cttggtcacc gtactgcgca accggaccgg acattgcgcc
    13861 cacgaccgcg actttaatat cttctgccag agccatattg ctgaatgcca gcgcgataca
    13921 tcctgccagt aacgctttac cctttatgtt catcctgaga atccccattc ttctggttat
    13981 tacgtgtgtt gtgatgttgt tgttcagcac tttatttcgt tttatgcatg gctacccgtg
    14041 ctttagcagc atactctgct aaaacatacc cgatttttat gatattggaa tagctatttt
    14101 gacagtttat taacaatctg cgtggggatt ggcgttttgc cggaggggaa attgattatt
    14161 gtagaggccc aaaaaacaaa aaccccggac tcgcatccag ggttctctgc ttaatagcgg
    14221 aaattacgct tctatagcag cgcgcagttt tttcatcgcg ttcttttcca gctggcgtac
    14281 acgttcagcg gaaacaccgt aacggtcagc cagttcctgc aacgtggact tgttgtcttc
    14341 gtccagccag cgcgcgcgga tgatgtcctg gctgcgttcg tccagacctt gcatcgcgtc
    14401 ggtcagacgg tttgccgcct gctcttccca gttatcatct tcgatgccgt cggcaaagtt
    14461 agatgattta tcctgcagat agagcaccgg tgccatcggc tggctgtcgg aatcgtcgtc
    14521 ggaagacagg tcaaaggtca tgtcctgtgc cgccatacgt gattccatct cacgaacgtc
    14581 tttgctggtt acgcccagtt cacgggccac catttcgact tcatcctggt taaaccagcc
    14641 cagacgctgc ttggttttac gcaggttgaa gaacagtttg cgctgcgctt tggtggtcgc
    14701 aactttgacg atacgccagt tacgcagaac gtattcgtgg atctctgctt tgatccagtg
    14761 aacggcgaag gagaccaggc gcacacccac ttccgggttg aaacggcgca ctgctttcat
    14821 caggccgatg ttaccttcct gaatcaaatc cgcctgtggc aggccatagc ccgcataatt
    14881 acgagcaata tgaacaacaa accgcaggtg agacaggatc agcgttttag ctgcttccag
    14941 atcgccatgg taatgcagct tttcagccag cgcccgctcc tcgtcagccg acaacatcgg
    15001 ccacgcgtta gctgcccgga tgtaggaatc caggttgcca actggggcta aagctaaact
    15061 ttgcattttg tcagtcattc aaatcctctc aatcgatatc ttctggcgct tcagtggtag
    15121 caacaactgt gccagagctt aagagcaacg aggttatcat ccactgtttt atcagacagt
    15181 gattttatcc acaagttcaa tgcaagcttg tgaataaatt acgcacaaaa tgtgacatag
    15241 agatgaaata ccgggaagag acaacgggat ctctttccct gctacggaac ccattgcagg
    15301 gaaagagtat aacacgcttt tattattcag gcgtaaagtg gcgtaaatgt tgtaccgtgg
    15361 caagccacgc tgccacccag ccaatcatcg agcataccag cagcaatagc aggcattcat
    15421 cgaacgataa accattgata tcaaacttcg ttccgaaaac ctgtgccact tccgcaaccg
    15481 ccgatgacaa tcgcagcacc agaatttctg acaaaattaa tgacaacaat gcgccagaaa
    15541 atcccagcag tgcgccacca tacaggaacg ggcgaaggat gaaaccatcc gtcgcaccaa
    15601 tcagtttctg cacgttgatg gagtcacggc gagcaaagat gctcagacgt acgctgttgc
    15661 cgatgacgag gaacactgcc gccaccatca acacgccgat catcgccgaa acgcgcccga
    15721 ccagcccggt caacgccgcc agacgggcaa accagctgtc atccatccgc acttcgtcaa
    15781 tgccgttaat ctgcgtgata cgatcacgca gcgtattgag tgattccgtc ccctgaaaat
    15841 cgagtttcgg gatcaccacc gccactgccg gaagcgggtt ttcttccagc atatccagcg
    15901 caccaccaaa accagaccag ttacggaact cgcccagtgc gtcttcacga gaaagatagt
    15961 tcactttctc cacgccttgc tcggcctgca actgtgccac cacgcccgca gcggcgtcat
    16021 cgtccagcgt tttttgcaag taaacagtga tttgcggtga cggataatac tgcgtcgccg
    16081 cctgattaac gtttttgtac accatataac agacgctggg cagcgtcagg gagatggcga
    16141 taaccatcac cgttaaaaac gtggcgaacg gtttgctttt cagatcctgc aatgcgccgt
    16201 ggaaggcata gcgcacctgt tcgttgaaaa cgttggtttt gcgatttacc ggttttggcg
    16261 aggatttcgc gcgtttcggt gcgttacgac cgccgtcgcc tgagccgccg accgatttac
    16321 ggaagcgatc cagacgccca ccaaactgcc gaatatgatt gattgcatcg cgcttattca
    16381 tggcccacgc ctccatgcaa gtgaccatcg ctcagggtga gcatgcgatg ggaacgccgt
    16441 gaaatcaggt tgatgtcgtg cgttgccatc aatacggtta ccccaacgcg gttaaactct
    16501 tcaaacagac gtagaatccc ttccgacaac gcgtcgtcca ggttaccggt cggttcgtcc
    16561 gccagcagta ccgcgggctt attcaccacc gcgcgggcaa tgccaacacg ctgttgttca
    16621 ccgccggaaa gctgaatagg gaagttcttc gctttgtcca gtagcccgac tttatccagt
    16681 gccgccgaca cccggcgacg aatatcgtca ccgctggcac cagcgataat cagcgggatc
    16741 gccacgttat cgtagacagt acggtccatc agtagatggt gatcctggaa aatcatgcca
    16801 atctggcggc gcagaaacgg aacttcacgg tttttcagac gcgtgatgtc atggccgcta
    16861 aaccagattt tcccggcgct gggccgctca atcccacaga tcagcttcag gagggtactt
    16921 ttccctgcgc cggaatgacc ggtcagaaac gccatctcac ccggctgcat atggaacgta
    16981 acgccctgca gcgcctgtct cccaccgaga taagccttgc tgacatgttc aaagcgaatc
    17041 attgttaatc ctctcgggca aaaagtgcct ctataaagtc gtccgcctta aacggacgca
    17101 aatcctcaat acgttcgccg acaccaatgt agcggatagg gataccaaac tggtcagcca
    17161 ccgagaaaat taccccgcct ttcgccgtgc cgtccagttt cgttagcgtg atgccggtta
    17221 agccaacagc ttcgtggaac agtttggcct ggcttaccgc gttctgcccg gtgctggcat
    17281 caatagtcag cataacttca tgcggcgctt caacgtcgag tttcttcatc acgcggacga
    17341 ttttcttcaa ctcttccatc aggtgcgatt tgttctgcag gcgtccggcg gtatcggcaa
    17401 tcaggacgtc gatattacgc gctttagctg cctgaatggc gtcgaagata acagaggcgg
    17461 aatccgcccc ggtatgctgg gcaatcaccg gaatattgtt gcgctgaccc cagacctgaa
    17521 gctgttcaac cgctgctgca cggaaagtat cgcccgccgc cagcatcacc gatttaccct
    17581 gctgctcaaa ctgacgtgcc agcttaccaa tcgtcgtggt tttacccaca ccgttgacgc
    17641 ccaccatcag gatcacgaac ggcgttttac cttcaacatt cagcggctca tcgactttcg
    17701 ccagaatctc acccatctct tctttcagca ggccatagag cgcctcggca tcacgaagct
    17761 gcttgcggga tgcgccttcc gtcagattgg tgataatttt acgtgtggtt tccacgccca
    17821 catcggcgat caaaagctgc tcttccagct cctcaaacag atcatcgtcg atttttttac
    17881 cgcggaacag gctgataaat ccggaaccga gattttcttt ggtttttaac aggctgcgtt
    17941 tcaggcgcgc gaagaaacct tctttggttg gtttttcctg ctcctgagcg atttcttcca
    18001 ccggctgctc ttcttctgcc ggaggaacca ccatcaccgc ctcttctgct gcttcggctg
    18061 ccagcgcctg tgcttccagc tcttcgtcgg tgatttcttc tttagccgct tcttcttccg
    18121 ccgctttgac aatctctacg gtttccgctt cagcctgcca ctcttctggc gaaacctctt
    18181 cggcgttgac gtcttccggc aacggcagct cttcacgttc gatagccact ggctccggcg
    18241 tttcttctac gaccggttcc ggctgtgcaa caacttccgc ttcaggctgc gctttttcac
    18301 tttcaacaac ctgttcagtg acttccacaa cgtcggcagc aaaagtttcc gcctcggctt
    18361 cagtatgcgc ctgcggctgc tcttcaacgg cttgttccga agccttcaca ggctcttgcg
    18421 cctgaacgat ttcttctaca accggttgtt cattctgaac ttctgtctct ttttccgggg
    18481 tctgctcttt ttgaccaaag cccagccagg aaaaaaagcc acgttttttt tctttcgcca
    18541 tttgcgacta cactcctcgc tgttgcttca tggcacagcg ttaacgctat gtacatagca
    18601 gctaaaaaaa tgatgaaata gtctatcact taacttaatt cacatcaccg cctgcaagta
    18661 tgtgttatct ggcggattga gcaatttatc atgaaaaaac cgaatcattc cggcagcggc
    18721 caaatccgca ttattggcgg gcagtggcga ggccgtaaac tcccggttcc tgatagccca
    18781 ggtctgcgcc ccaccaccga ccgcgtacgc gaaacgttgt ttaactggct ggctccggtc
    18841 attgttgacg cccaatgtct ggattgcttc gccgggagcg gcgcgctggg gctggaagcg
    18901 ttatcgcgct acgctgcggg ggccacgtta attgagatgg atcgtgcagt ttctcagcag
    18961 ttaattaaga atctggcgac actaaaagca ggcaatgcac gcgtggtgaa cagcaacgcg
    19021 atgtcattcc tggcgcaaaa aggcacaccg cataatatcg tgtttgtcga tccaccgttc
    19081 cgccgtggct tgttagaaga gacgataaat ttactggaag ataacggctg gctggctgac
    19141 gaagccctga tttatgtcga aagcgaagtc gaaaacggtc tgcccaccgt tccggcaaac
    19201 tggtcgttac atcgggaaaa agtggcgggt caggtggctt atcggctgta tcaacgcgaa
    19261 gcacaaggag aaagtgatgc tgattaatat tggtcgtttg ttaatgctct gcgtttgggg
    19321 atttttaatc ctcaatctgg tgcatccctt cccgcgcccg ctaaatattt tcgttaacgt
    19381 ggcgctgatt tttaccgtgc tgatgcatgg tatgcagctg gcgctattga aatccacttt
    19441 accgaaagat ggcccgcaga tgaccaccgc cgaaaaggta cggattttcc ttttcggcgt
    19501 gtttgaactg ctggtctggc agaagaaatt taaagttaaa aaataacctt attgttcgcc
    19561 aacaaagcta acaaagcgcg ttcctttata gctcagcgta cctttatcgc ccactgtcag
    19621 ggcgtggtac tgctgggcgt cgaggcgaaa cgtctgctcc attcctccgc tttgcggttt
    19681 gaagcttgcc tcgtagcgca tactggtgcc tgccggagtc acttcctgct ggcgcgaacg
    19741 gcgatcgtta atcggttttt cccgcttgtt gctcaccacc accagctttt gctggagcgg
    19801 agccatatca ttatcagctt tttcccgtcg ctgttgcata aaacgaaacg atgcggcgac
    19861 gacaattaag ccaatgataa caataaagaa aagaggtggt ttgctcatct ttatccctca
    19921 tcggaaaatg cggaaataag cataccctgc cagttatggt gttgtcatcc gtccaccctc
    19981 gccactaaac tggaagcaag accgtaggca ttccgcttac gaaaaaataa cgaattcaag
    20041 gaactaagat gctttggtcg tttatcgctg tctgtctttc cgcatggcta tctgtggatg
    20101 catcgtatcg tgggccaacc tggcaacgct gggtgtttaa accgttaacc cttcttctcc
    20161 tgctgttact ggcctggcaa gcgccgatgt tcgacgccat tagctatctg gtgctggcag
    20221 ggctgtgcgc ctcactgctg ggcgatgcgc taaccctgtt gccacgtcaa cgtctgatgt
    20281 acgccatcgg cgcgtttttc ctctcgcacc tgctgtacac catctatttc gccagtcaga
    20341 tgacgctctc tttcttctgg cctctgccac tggtgttgct ggttctgggt gcgctgttac
    20401 tggcgattat ctggacgcgg ctggaagagt atcgttggcc tatctgcacg tttatcggca
    20461 tgacgctggt gatggtgtgg ctggcaggtg aactgtggtt cttccgtccg accgctccgg
    20521 cgctctctgc gtttgttggc gcttcgttgc tgtttatcag taactttgtc tggctgggga
    20581 gccactatcg ccgacgcttc cgtgcggata acgcgattgc tgcggcctgc tactttgccg
    20641 gtcacttcct catcatccgc tcactgtatc tctgataaaa cttgactctg gagtcgactc
    20701 cagagtgtat ccttcggtta atgagaaaaa acttaaccgg agtatgccat gtcgacacct
    20761 gacaatcacg gcaagaaagc ccctcaattt gctgcgttca aaccgctaac cacggtacag
    20821 aacgccaacg actgttgctg cgacggcgca tgctccagct cgccaacgct ctctgaaaac
    20881 gtctccggca cccgctatag ctggaaagtc agtggcatgg actgcgccgc ctgtgcgcgc
    20941 aaggtagaaa atgccgtgcg ccagcttgca ggtgtgaatc aggtgcaggt gttgttcgcc
    21001 accgaaaaac tggtggttga tgccgacaat gacattcgtg cacaagttga atctgcggtg
    21061 caaaaagcgg gctattccct gcgcgatgag caggccgccg acgagccaca agcatcgcgc
    21121 ctgaaagaga atctgccgct gattacacta atcgtgatga tggcaatcag ctggggtctg
    21181 gagcagttca atcatccgtt cgggcaactg gcgtttatcg cgaccacgct ggttgggctg
    21241 tacccgattg ctcgtcaggc attacggctg atcaaatccg gcagctactt cgccattgaa
    21301 accttaatga gcgtagccgc tattggtgca ctgtttattg gcgcaacggc tgaagctgcg
    21361 atggtgttgc tgctgttttt gattggtgaa cgactggaag gctgggccgc cagccgcgcg
    21421 cgtcagggcg ttagcgcgtt aatggcgctg aaaccagaaa ccgccacgcg cctgcgtaac
    21481 ggtgagcggg aagaggtggc gattaacagc ctgcggcctg gcgatgtgat tgaagtcgcc
    21541 gcaggtgggc gtttgcctgc cgacggtaaa ctgctctcac cgtttgccag ttttgatgaa
    21601 agcgccctga ccggagaatc cattccggtg gagcgcgcga cgggcgataa agttcctgca
    21661 ggagccacca gcgtagaccg tctggtaacg ctggaagtgc tgtcagaacc gggtgccagc
    21721 gccattgacc ggattctgaa actgatcgaa gaagccgaag agcgtcgcgc ccccattgag
    21781 cggtttatcg accgtttcag ccgtatctat acgcccgcga ttatggccgt cgctctgctg
    21841 gtgacgctgg tgccaccgct gctgtttgcc gccagctggc aggagtggat ttataaaggg
    21901 ctgacgctgc tgctgattgg ctgcccgtgt gcgttagtta tctcaacgcc tgcggcgatt
    21961 acctccgggc tggcggcggc agcgcgtcgt ggggcgttga ttaaaggcgg agcggcgctg
    22021 gaacaactag gtcgtgttac tcaggtggcg tttgataaaa ccggtacgct gaccgtcggt
    22081 aaaccgcgcg ttaccgcgat tcatccggca acgggtatta gtgaatctga actgctgaca
    22141 ctggcggcgg cggtcgagca aggcgcgacg catccactgg cgcaagccat cgtacgcgaa
    22201 gcacaggttg ctgaactcgc cattcccacc gccgaatcac agcgggcgct ggtcgggtct
    22261 ggcattgaag cgcaggttaa cggtgagcgc gtattgattt gcgctgccgg gaaacatccc
    22321 gctgatgcat ttgctggttt gattaatgaa ctggaaagcg ccgggcaaac ggttgtgctg
    22381 gtagtacgta atgatgacgt gctgggtatc attgcattgc aggataccct gcgcgccgat
    22441 gctgcaactg ccatcagtga actgaacgcg ctgggggtca aaggagtgat cctcaccggc
    22501 gataatccac gtgcagcggc ggcaatcgcc ggggagctgg ggctggagtt taaagcgggc
    22561 ctattgccgg aagataaagt caaagcggtg accaagctga atcaacatgc gcccctggcg
    22621 atggtcggtg atggtattaa cgacgcgcca gcgatgaaag ctgccgccat tgggattgca
    22681 atgggcagtg gcacagacgt ggcgctggaa accgctgatg cagcattaac ccataaccac
    22741 ctgcgcggcc tggtgcaaat gattgaactg gcacgcgcca ctcacgccaa tatccgccag
    22801 aacatcacca ttgcgctggg gctgaaaggg atcttcctcg tcaccacgct gttagggatg
    22861 accggcctgt ggctggcggt gctggcagat acgggtgcga cggtgctggt gacagcgaat
    22921 gcgttaagat tgttgcgcag gagataaggc aaaccgatcg caacattgag cgcgatcggt
    22981 cccctcgccc ctctggggag agggttaggg tgaggggaaa aggcggcatc gaagccaatc
    23041 tttccccatc aaccgccttt acgaatcaaa taacgataag gcagtccatc cgtctctttc
    23101 gcaaccagtt cgtgttccat aaaggtacaa aacccaggaa tatcgcgggt agtggccgga
    23161 tcgtcggcga taatcagcaa cgtttcgcca ggctgcatat tgcgcacagt tttgcgcacc
    23221 atcattacgg gttccgggca gcgcaggcca agtgcgtcga gtgtgtggtc agggctggaa
    23281 aagagatcgg tcattttctt ctcatcacat aaaaaaacgg cgctagttta cgccctgtga
    23341 gtccgtaagc aaaccaggtt aacgattgcg tgaaaattaa ccattgcatt gtcaacgtaa
    23401 agcagtatca tgcggcggct cgaaaaaagg gtaagcacgt tattatgtta aggtaacaga
    23461 cgtgtcgtac gtattgggtt ccctcacccc aatggttaat caaaaaggta caatatgaac
    23521 gttttctcgc aaactcaacg ctataaggcg ttgttctggt tatcgttatt tcatctgctg
    23581 gtgatcacct ccagtaacta tctggttcag cttcccgtct ccattttggg tttccatacc
    23641 acctggggcg cgtttagctt cccgtttatt ttccttgcta ccgacctgac cgtgcgtatt
    23701 tttggcgcac cgctggcccg tcgtatcatc tttgcggtga tgatcccggc gttgctgatc
    23761 tcctacgtca tctcgtcgct attctatatg ggttcctggc agggattcgg cgcactcgcc
    23821 cacttcaacc tgtttgtcgc ccgtatcgcc accgccagtt tcatggccta cgcgctggga
    23881 cagatcctcg acgtgcacgt ttttaaccgc ctgcgtcaga gtcgccgctg gtggctggca
    23941 ccgacagcgt ccacactgtt cggtaacgtc agcgacacgc tggccttttt cttcattgcc
    24001 ttctggcgta gcccggatgc ctttatggct gaacactgga tggaaatcgc gctggtcgat
    24061 tactgtttca aagtgttaat cagtatcgtt ttcttcctgc caatgtatgg cgtattactc
    24121 aatattctgt tgaaaagact ggcagataaa tccgaaatca acgctttgca ggcgagttaa
    24181 aggttcgtta tcagagttgt gataagatgg atgaatgagc cgttatggcc gtttatcgaa
    24241 aggaagaagt caatgcgcaa tctggttaaa tatgtcggaa ttggcctgct ggttatgggg
    24301 cttgcggcct gtgatgataa agacactaac gctacggcgc agggttcggt cgcggaaagt
    24361 aacgctaccg ggaatcccgt caacctgctt gatggcaagt taagtttctc gctgccagcg
    24421 gatatgaccg accagagcgg taagctggga acgcaggcca ataacatgca tgtctggtcc
    24481 gacgccaccg ggcagaaagc agtcatcgtc atcatgggcg atgatccgaa agaagatctg
    24541 gcggtgttgg cgaagcgtct ggaagatcag caacgtagcc gcgatccgca gctgcaagtg
    24601 gtaaccaaca aagccattga gctgaaaggt cacaaaatgc agcagttaga cagtattatc
    24661 tccgcgaaag gccagacggc gtactcttcc gttattctgg gtaacgtggg taatcaactg
    24721 ctgaccatgc aaattactct gccagctgac gatcagcaaa aagcgcagac caccgcagaa
    24781 aacatcatta atacgctggt tattcagtaa gttttaagat gatgaggcgg cctcagggac
    24841 gtgttccgga ggccgttttt ttaatcgcca cgtcagtaat aacgcgattg cgaccagtcc
    24901 cgccgccgcc agataaatca ccggtacgcc cgcccagctc atcaccagcc cagccagtgg
    24961 tccggtcacg ccaagcgata aatccataaa tacggtgtaa gttgccagcg ccgccccctg
    25021 attttgctgc ggaaccgctt ttaccgccac tacgcccaat gccgggaaca ccagcgaaaa
    25081 ccctgccccc gccagtaaga cgccgacttt cgccatccac ggcatagtcg ccacgccaac
    25141 cagtagcagg ccgattatct caacgctaaa gcaaatcatc gccacgttta agccgccgat
    25201 acggttaatg ccgttaggga ataacaaacg cgtaccgaca aacgcacagc taaacagcgt
    25261 cagcgcgaaa gccgcaccgt cccaaccttt agcgtcataa aacagcgtga taaaggtggc
    25321 gatgacgcca aatccggcag aagccagtgc cagtgccata ccgtacagcc agacgcgccc
    25381 aagcaccgcg cgaaacggca gcggtttgcc tttactggct tttaccgtcg gacgcgggat
    25441 cgccaacaaa atggccacca gcgccacgcc cataatgatt aacgctaacg cctgcaagcc
    25501 gccccagtga taaaacacga cgcctaacgg cgcacccatc gccatcgccc cgtaattgac
    25561 aatgccgttc cacgaaatca ctcgcccgat atgcagcgag ccaaccacgc caacgcccca
    25621 cagggtcgat cccgttccgg caaaactttg cccaatccca aggatcacgc gtcccaggca
    25681 aagtaataac aggctgatga cgggcagact ggcggttaat cctgccgtca gatatcccag
    25741 accgctcaaa aagcagccgc ataaaccgaa gacgacaatc tttttggggc ccagcaaatc
    25801 ggcgtaacgt ccggcatgag gacggctcag caaggtggcg aaatattgca ggctgataac
    25861 caatcctgcc cagaaagcgc taaagcccat cacatcatgg acatagcccg gtaatacagc
    25921 gagcggcaac ccgatggtga ggtagctggc gaagttaaac atgacaatgg agacaatgcg
    25981 caaattcagg cgcaatccgt ttagcgcggg ttcggctacg ggttcgggca tgaggatcac
    26041 cacattttta caacagtgtt tcatttttac cacgtgctga cgtgaaaatc agcagtaaga
    26101 atcagaatat tgctggcgtg actcccgcta cacttaatac aaaaagtcac aaggaagccc
    26161 caatggaaat ccctcaaccc gataaaacgg gcatgcacat tctgctcaag ctggcctcgc
    26221 tggtagtgat cctcgcgggc attcacgcag cggcagatat cattgtgcag ctgttactgg
    26281 cgctgttttt tgccatcgtc ctcaacccgc tcgttacctg gtttattcgt cggggagtac
    26341 aacgccccgt tgccattacg attgtagtgg tggtgatgct gatcgcacta accgcgctgg
    26401 tcggcgtact ggcggcatcg tttaacgaat ttatctctat gctgccgaag tttaataagg
    26461 agctgacgcg caaacttttt aaattgcagg agatgttgcc ttttcttaat ttgcatatgt
    26521 cgccggagcg aatgctgcag cggatggact cggaaaaagt ggttaccttc accacagcgc
    26581 taatgaccgg gctttccggg gcaatggcga gcgtgctttt gctggtgatg accgtagttt
    26641 ttatgctgtt tgaagtgcgc cacgtccctt acaaaatgcg ttttgcgctg aataacccac
    26701 agattcacat cgcaggatta caccgcgcac ttaaaggcgt ttcgcactat cttgcattga
    26761 agacgctact cagtttatgg acaggtgtca tcgtctggct ggggctggag ctgatggggg
    26821 tgcagtttgc gctgatgtgg gcagtactgg cgtttttgct caactacgtg cccaatatcg
    26881 gcgcggtaat ttccgccgta ccgccaatga ttcaggtgct gctgtttaat ggtgtttacg
    26941 aatgtattct ggtcggcgca ttgtttttag tggtccatat ggtcatcggc aatattttag
    27001 aaccacggat gatgggccat cgcctgggga tgtccaccat ggtggtattt ctttcattgt
    27061 taatttgggg atggctgctc ggcccggtag ggatgctact ttcggtgcca ttaaccagtg
    27121 tgtgtaaaat ctggatggaa accaccaaag gcggtagcaa actggcgatt ttactggggc
    27181 caggcagacc gaaaagtcgg ttaccgggat gaggcgacaa gtgatacgat acgcactttc
    27241 attttccatt aaacgttggc cctgatatgt atcggatagt tctggggaaa gtttcgacct
    27301 taagcgcagc tccactgcca ccgggtttac gcgagcaagc accgcaaggt ccacgacgcg
    27361 aacgctggct ggcggggcgt gcattgcttt cgcacacgct ttccccgcta ccggagatca
    27421 tctatggcga acaaggcaaa cctgcattcg cgccggaaac gccgctatgg ttcaacttaa
    27481 gccatagcgg tgacgatatc gctctgctgt tgagcgatga aggcgaagtc ggctgcgata
    27541 tcgaagtgat tcgcccgcgc gccaactggc gctggctggc gaacgccgtg ttcagcctcg
    27601 gggaacacgc tgaaatggac gccgtgcatc ctgatcagca actggaaatg ttctggcgca
    27661 tctggacgcg caaagaagcc atcgttaaac agcgtggcgg cagcgcctgg caaatcgtca
    27721 gcgtagatag cacctatcac tcctcgctgt cagtcagcca ttgtcagcta gaaaatttaa
    27781 gccttgcgat ctgcaccccc acccccttta cgctcaccgc cgacagtgtg caatggatcg
    27841 attcagttaa ctgatccgcc cacccgactg cccatctatt gatccagaac aggtaatcag
    27901 tatgacgaat acttaaaatc gtcatactta tttccgccat ctattttaat ccattggggt
    27961 taccatgctc tccacactcc gccgcactct atttgcgctg ctggcttgtg cgtcttttat
    28021 cgtccatgcc gctgcaccag atgaaatcac taccgcctgg ccggtgaatg tcgggccact
    28081 aaacccgcac ctttacacgc ctaaccagat gttcgcccag agcatggttt atgaaccatt
    28141 ggtgaaatat caggcagacg gttcggtgat cccgtggctg gcaaaaagct ggactcattc
    28201 agaagatggt aaaacctgga ccttcaccct gcgtgatgac gtgaaattct ccaacggtga
    28261 accgttcgat gccgaggcgg cggcagaaaa cttccgcgca gtgctcgata accgtcaacg
    28321 tcacgcctgg ctggagctgg caaaccagat tgttgatgtt aaagcactca gtaaaacaga
    28381 gctgcaaatt accctgaaaa gcgcctacta tcctttcctg caagaactgg ccctgccccg
    28441 tcctttccgt tttatcgctc cctcgcagtt taaaaaccat gaaaccatga acggaattaa
    28501 agcgccgatt ggcaccggac cgtggatttt gcaggaatcg aaactgaatc agtacgatgt
    28561 cttcgtccgt aacgaaaact actggggcga aaagccagcg attaaaaaga tcacctttaa
    28621 cgtcatcccg gacccgacta cccgcgcggt ggcgtttgaa actggcgata tcgacctgct
    28681 gtacggaaac gaagggttat taccgctcga taccttcgcc cgctttagcc agaatccggc
    28741 ttaccacacc caactgtcac agccgatcga aaccgtgatg ctggcgctca ataccgccaa
    28801 agcccccacc aacgagctgg cagtacgtga agctcttaat tacgcggtaa acaaaaaatc
    28861 gctgattgat aacgcgttat atggcaccca gcaggtcgcc gacaccctgt ttgccccttc
    28921 tgtgccttac gctaatctcg gcctgaaacc gcgccagtac gatccgcaga aagcgaaaga
    28981 gttgctggaa aaagccggtt ggacgctgcc tgcgggcaaa gacatccgcg agaaaaatgg
    29041 tcagccgctg cgcattgaac tgtcgttcat cggcaccgat gcgttaagca aatcgatggc
    29101 ggaaatcatt caggctgata tgcgccagat tggcgcagat gtctcgctga ttggcgaaga
    29161 agagagcagt atctatgctc gtcagcgcga cggtcgtttt ggcatgattt tccaccgcac
    29221 ctggggcgcg ccatacgatc cacacgcctt cctgagttca atgcgcgtac cgtcgcacgc
    29281 tgacttccag gcacagcaag gattagccga caagccgctg attgataaag agatcggcga
    29341 agtgctggcg acccatgacg aaacgcaacg tcaggcgctg tatcgcgaca ttttgacccg
    29401 tctgcatgac gaggcggttt atctgcctat cagctacatc tcgatgatgg tggtatcaaa
    29461 accggagctg ggtaatatcc cctacgcgcc gatcgccacc gaaattccgt tcgaacagat
    29521 taaaccggtg aaaccttaat gttgcgttac gtattacgcc gctttctgct gctgatcccg
    29581 atggtgctcg ccgcctcggt gatcattttt ctgatgctgc gcctcggtac cggcgacccg
    29641 gcgctcgatt atctgcgttt atctaacctg ccgccgacgc cggagatgct ggcctctacc
    29701 cgcaccatgc tcgggctgga tcagccgctg tacgtccagt acggtacctg gttgtggaag
    29761 gcgctgcatc ttgactttgg tatctcattc gccagccaac gcccggtgct ggacgatatg
    29821 ctgaacttcc tgcccgccac gctggaactt gcaggtgcgg cgctggtatt aattctgctc
    29881 acttccgtac cgctcggtat ctgggcggcg cgccatcgcg accgtctgcc ggatttcgcc
    29941 gtacgtttca tcgcgtttct tggcgtgtcg atgcctaact tctggctggc gtttttactg
    30001 gtgatggcgt tttcggtgta tctgcaatgg ctacccgcga tgggttacgg cggctggcag
    30061 cacatcattt tgcctgcggt ttccattgcc tttatgtcgc tggcgattaa cgcgcgactg
    30121 ctgcgcgcca gtatgctgga cgtcgccggt cagcgtcacg tcacttgggc gcgtctgcgc
    30181 ggcctgaacg acaaacagac cgaacgccgc catattctgc gcaatgcctc gctgccgatg
    30241 atcaccgccg tggggatgca catcggcgaa ctgattggcg ggacgatgat tatcgaaaac
    30301 atctttgcct ggccgggcgt cgggcgctat gcggtgtcgg cgatttttaa ccgtgactat
    30361 ccggtgattc agtgctttac gctgatgatg gtggtggttt ttgtggtctg taatttgatt
    30421 gtcgatttgc tcaacgccgc gctggacccg cgcattcgtc gtcatgaagg agcgcacgcg
    30481 tgaacttttt cctctcttcc cgctggtcgg tacgcctggc gctgatcatt atcgccctgc
    30541 tggcgctgat tgcgctcacc agccagtggt ggctgccgta tgacccccag gcgattgatt
    30601 tgccgtcgcg cctgctttcg ccggatgcgc agcactggct gggcaccgat cacttaggtc
    30661 gcgatatttt ctcgcggctg atggcagcga cccgcgtgtc gctcggttcg gtaatggcct
    30721 gcctgctgct ggtgctgaca ttagggctgg ttattggcgg cagcgccggg ttgattggcg
    30781 ggcgcgttga tcaggccacc atgcgcgtcg ccgatatgtt tatgaccttc ccgacctcga
    30841 ttctgtcgtt ctttatggtt ggcgtgctcg gcaccgggct gaccaacgta attatcgcca
    30901 tcgccctgtc gcactgggcg tggtatgcac gcatggtgcg cagcttggtg atttcactac
    30961 gccaacgcga gtttgtgctg gcgtcacggc tttccggtgc gggccatgtg cgggtgtttg
    31021 tcgatcatct ggcaggcgcg gtgatccctt cgctgctggt gctggcaacg ctggatatcg
    31081 gccatatgat gctgcacgtc gcggggatgt ctttccttgg cctcggtgtg accgcgccga
    31141 ccgccgaatg gggcgtgatg attaacgacg cgcgccagta tatctggacc cagccgctgc
    31201 aaatgttctg gccggggctg gcgctgttta tcagcgtgat ggcctttaac ctggtgggtg
    31261 acgcactgcg cgatcatctg gatcctcatc tggtgacgga gcacgcacac taatgccgca
    31321 acagattgaa ctacgtaata tcgcgctaca ggccgcgcag ccgctggtac acggtgtatc
    31381 gttaaccctg caacgcgggc gcgtgctggc gttagtcggc ggtagcggca gcgggaaatc
    31441 attaacctgc gccgcgacgc tgggcatttt gcccgctggc gttcgccaga cggcggggga
    31501 aattttagcc gatggcaaag cggtttatcc ctgcgccctg cgcggcatca aaattgccac
    31561 catcatgcag aacccgcgca gcgcctttaa tccgctacac accatgcaca cccacgcgcg
    31621 ggaaacctgc ctggcgttag ggaaacccgc cgatgacgct acgcttaccg ctgccataga
    31681 agcggtgggg ctggaaaacg ccgcgcgcgt gctgaagctg tacccgttcg agatgagcgg
    31741 cggcatgttg cagcgcatga tgattgcgat ggcggtgctg tgtgaatcac cgtttatcat
    31801 cgccgatgaa ccgaccaccg acctcgacgt ggtagcacag gcgcgcatcc tcgatctgct
    31861 ggaaagcatt atgcaaaaac aagcgccggg aatgctgctg gtcacccatg atatgggcgt
    31921 tgtggcgcgt ctggcggatg acgtggcggt gatgtcacaa ggtaaaattg tcgaacaggg
    31981 cgatgtagaa acgctgttta acgcccccaa acatgcggta acgcgcagcc tggtttccgc
    32041 tcatctcgcc ctctacggta tggagctggc atcatgactt tacttaacgt ctccggcctt
    32101 tcccatcact atgcccacgg tggatttaac ggaaaacatc aacatcaggc ggtgctgaat
    32161 aacgtttccc tgaccctgaa aagcggcgaa accgtcgccc tgctggggcg cagcggctgc
    32221 gggaaaagta ccctcgcgcg gttgctggtg ggtttagaat cgcccgcgca ggggaatatt
    32281 agctggcgtg gcgaaccgct ggcgaaactc aatcgcgccc agcgtaaagc gttccgccgc
    32341 gatattcaga tggtatttca ggactccatc agcgccgtga atccgcgcaa aaccgtgcgc
    32401 gagatcctgc gtgaaccgat gcgccacctg ctatcactga aaaaatccga acaactggcg
    32461 cgcgccagcg aaatgctgaa ggcggtcgat ctcgatgaca gcgttctcga caaacgccca
    32521 ccgcagttaa gcggcggcca gctccagcgc gtctgcctgg ctcgcgcgct ggcggtcgaa
    32581 ccgaaactac tgattctgga tgaagccgtt tctaaccttg atctcgtgtt acaggcgggc
    32641 gtcattcgcc tgctgaaaaa gctacaacaa cagtttggca ccgcctgcct gttcatcacc
    32701 cacgacttac gcctggtgga acgcttctgt cagcgggtga tggtgatgga caacggacaa
    32761 atcgtcgaaa cccaggtggt gggagataaa ttaacctttt cctctgacgc cggacgtgtg
    32821 ctacaaaacg cggtattacc cgcattcccc gtgcgccgtc gcaccacaga aaaggtttaa
    32881 cgcaaatgca acgagtcacc atcacgcttg atgacgattt actggagacg ctggacagcc
    32941 tgagccagcg tcgtggttat aacaaccgtt ccgaagctat ccgcgatatt ctgcgtagcg
    33001 ccctggccca ggaggccact cagcagcacg gcacgcaagg tttcgcggtg ctctcctatg
    33061 tgtatgaaca cgaaaaacgc gacttagcca gccgcattgt ctccacccag caccatcacc
    33121 acgatctctc cgtcgccacg ctgcatgtgc atatcaatca cgacgactgt ctggaaatcg
    33181 ccgtgctgaa aggtgatatg ggcgacgtgc agcattttgc cgatgacgtt atcgcccagc
    33241 gcggcgtgcg gcatgggcat ttgcagtgct tgccgaagga agattgagtt atttctgaaa
    33301 cgaggaaaga aaagatatta actgaactta atttataaaa ttaagttcag ttgcatagat
    33361 agatcctgta aaggcagata ttctgcgtaa tgtacgccgc taaaatttat aaatgattca
    33421 aacgctcatt cagtgtttca ataatatagg tattaacaga gacttgttgc tgcgcagccg
    33481 cattattaag gcgttcactc agcgattctg gatagcgtaa cgtaaaggtc ttaatctttt
    33541 ctgtacgcgc atacggctca atacccgcag ctttacaatc ctcaagatac tctcgcaggg
    33601 aaagttcccc ttctttttgc agcccctgaa tgctgtcaga gacaaaatca caataacctg
    33661 acaagcccag gaatttgcca cgaaatgcat tcagttctgg cacgtaggta ataacagctg
    33721 gttgcccggc aatctccata gagtttggcg tcttcagttt gatcatggtt ttacccctat
    33781 acttagtaac cagtcacgca cgctttctac agcgccttta tcggtatctg gcgacggatg
    33841 aggccgatgg aaacacgcaa cgctcatatt tagtatgaac ttacaacgcg aacctcttcc
    33901 ttccttaatt tctccgccta atgctttaac cagtgactct atatcagacc acttgatccc
    33961 ttgaggaaca ggagttttaa atatttgatc caatgtattt ttttttcttc cgtaaagcct
    34021 gtaccttggt tctcatggca tgtccttatg acttcacttt atgacatcaa tagtatgaca
    34081 tcacagtatg aagtcaagtt tcccttcctt acgccattgt cccaatcgtc ttcctgaatc
    34141 gcagcagcgc aatggtgaaa aacgcgccgc caattgccat cagcgtcagg aactgcggcc
    34201 agacgatttc gaatccggca ccccggtaga gaatggcctg cgcgaggcta acaaagtgtg
    34261 tcgtcggcat ggtcagcata atgtcctgca ccatctgcgg catactttcg cgcggcgtgg
    34321 atccaccgga aagcatttgc agcggcagca gcaccagaat caccagcagc cccagttgcg
    34381 gcattgaacg cgctatcgtc cccataaaaa tgccgattga cgtggtggca aacagactga
    34441 gcgccacgcc cagcataaac agcgggatcg agccttcaat cggtacgccc agtacacctt
    34501 tcgccatcag caccagcgat aatcccgata ccaccagcac caccagcccc atcgaccaga
    34561 tcttcgccat catgatctca aacggcgtta tcggcatcac cagtaagtgt tccaccgtgc
    34621 cgtgttcacg ctcgcggatc agcgccgatc cggtcaatac aatcgccagc atggtaatgt
    34681 tgttgatgat cgccatcacc ccgccaaacc acgcgggatc gaggttcggg ttaaagcgca
    34741 tccgggtttc cagcgatacc aacggttcgc tgttatcacg gtagcgcgcg acaaagctgt
    34801 tcacttcacc gttgataata ttctggatat acccattgcc ggtaaatgcc tggctcatgc
    34861 gcgtggcatc gacgttcacc tgaatatccg gctggcgtcc ggcgaggaca tcacgctgaa
    34921 aattaggcgg aatatttatc gcgaaggtat agcgtccggc gtccagtccg gcatccatct
    34981 catcggcggt gatcatctcc ggtggcaaaa accacggacg atagaagctg ttaacgatcc
    35041 ggttcgataa ctgcgattga tccatatcgg caatggcgat cggcgcgagg ttcaacgatc
    35101 ctggcgtaac ggtcgctgac gaatacaccg acaccgtaaa cgagaagaca atcagcgtca
    35161 gcatcgcttt atcaccgagc agactgcgca actctttgat acccagatta aaaatattgc
    35221 gtaaatggcg catcatccct cctgtttttt cagcagcagg atgcttaagc ccatcaccag
    35281 cgggatggct atcagcaacg ggataaaaag ttgccacaaa tcagtcagat ccagcgcttt
    35341 cgagaacgtt ccgcgggcga tagtcagaaa atgactggtc gggtaaacct cgccgatcca
    35401 acgtccaggc ccttccagcg aagctaccgg atcgatcatc ccggaaaact gtgtcgccgg
    35461 gatcaacgtg ataatcgccg ttccgaaaat ggcggcaatc tggcttttca taaaggtgga
    35521 gatcagcagc cccattccgg tggcaatgat gatatacagc agcgccgcca gggtgagcgt
    35581 caggaaactg cctttatgcg gtacgccaaa cacaaacacc gacaggccgc agagcaggaa
    35641 aaagttcagc atccccagcg cgatgtacgg cagctgtttg ccaagcaaaa attcgctacg
    35701 ggtggtgggg gtcacgtaaa ggttgataat cgaccctagc tctttttccc gcaccacgct
    35761 aagggcgctt agcattgacg ggatcatcat cagcagaagc gggatcaccg ccggaacaat
    35821 cgctggcagg ctttttacgt ccgggttata gcgatagcgc gtctcaatgt tcatcagccc
    35881 gctttggctg gcgggtgtcg attgtcggct cgccacatcc tgaagccaac tctggtgcat
    35941 tgcctgcacg taacctttta ccgtttcggc acgactgggc atcgctccgt cgatccagac
    36001 gccgagttcc acaggcgtac cacgcgcgat atcgcgcccg aaattgggcg ggatctcaat
    36061 cgccaccgtg atatcgcccg cacgcatccg acgatcaagc tcgtcataac tggtgagcgg
    36121 cggctgttcg ataaagtaac gggaaccgga gaggttgagt gtccacgcct ggctactgac
    36181 ggtctggtcg cggtcgagca ccgcaaagcg caggttttcc acatccatac tgatgccgta
    36241 acccattatc agcatcagga tcaccgttcc catcagcgcc agcgtcgaac gtactggatc
    36301 gcgtcgcagt tccagcgctt cgcggcggct gtagctaaac agacggcgca ggctaaatcc
    36361 ctgacgcggc gcgtgggtgg tgtcgtgtac cacgggcggc gcttcggctt cgttgctctg
    36421 ccctgccgct tcctgcaaat aggcgataaa tgcctcttcc agactggcgg ctccgcgttt
    36481 ctcaaccagt tcctgcggtg taccgctggc aagcactttt ccggcgtgca tcagtgagat
    36541 gcggtcgcaa cgttccgctt cgttcataaa gtgggtagag atgaagatag tcactttgtc
    36601 ctggcgcgag agatcgacca tcaactgcca gaacatatcc ctcgccaccg gatcgacacc
    36661 agaagtaggc tcatcgagga ttaacatctc cgggcgatga atcaccgcca ccgccagcga
    36721 aagccgctgg cgaatgccga gcggcaatga ctccggcaga acatcttcaa cgtcgttgag
    36781 cttaaaacgc tcgctcattt cagccactct tgcgggaatt tccgcttccg ggatgtgaaa
    36841 caaacgggca tgtaactcaa ggttttgccg cacggtgagt tcgttataga gcgaaaacgc
    36901 ctgcgacata tagcccaccc gacggcgggt atcgatatct tttggatcaa ccggttgccc
    36961 gaacagccac gcctcacctt cgctggcggg cagcagtccg gtgagcattt tcatggtggt
    37021 ggatttaccg cagccgttcg aaccaagaaa accaaaaatc tccccgcgtg gaatgcggaa
    37081 attaacgtga tcaacggcaa cgaaggaacc aaaacgcatg gtcagatcgc gcgcttcgat
    37141 ggcaatctct gcgttttcag gttgatacgg tgggatcact accgcctgat gcgcctggcg
    37201 ttgcgcttgc ggtaacagat ttataaatgc ttcttccagc gtagcgcttt gcgtttgctg
    37261 ccgtagctct tcggcgctgc cagttgccag cacttctccg gcattcatcg ctaccagcca
    37321 gtcgaagcgt tcggcctctt ccatataggc ggtggcgacc agcacgctca tattgctctg
    37381 ccgctggcga atactgtcga tcagatccca gaactgggag cgggagagcg ggtcaacccc
    37441 cgttgttggc tcatcaagga tcaacagttc cgggtcgtgg attaacgcgc agcacagccc
    37501 aagtttttgc ttcatcccgc cggagagttt ccctgccgga cgatcgcgaa acggtgctaa
    37561 ccccgtgctg gtcagcagct cattaattcg cacttcccgc tccgctttgt cgtgaccgaa
    37621 caggcgagcg aaaaaatcga cgttttcata caccgacaag gtgtggtaga ggtttttgcc
    37681 cagcccctgc ggcatccagg cgatgcgcgg gcagacgtcg cggcgatgcc tcgggtcgcg
    37741 catatcgccg cccagcacca tcacattgcc ctgctcaatg acgcgggcac cggaaatcaa
    37801 cgacaataag ctcgacttcc cgacgccatc cggaccaatc agcccgacca tacagcgggc
    37861 cggaatatcg agagtaatat tgttcagcgc aacgtttttt ccataatgct ggctcacgcc
    37921 cgccagttgc gcgacaggcg ggacgggaac cagttccaga tgcgtcattg cggcaacctc
    37981 accacgaggt cgtcaggcca cggaagttct tcattcaccc gcacccacgc tacgcccggt
    38041 aaaccggttt tgacatattc cagatgctgc tggagtaatt ccggtgggat acgcgctttg
    38101 acgcggaaca tcagtttcag ccgttcatcg ctggtttcga cggtttttgg cgtgaactgg
    38161 gcgacactgg cgacaaaact gatggtcgcc ggaatacgca gatctggcgc ggcatcgagg
    38221 atcagccggg cttcaccgcc aagtttcagc gtgcccgcct gttcggttgg caggaagaaa
    38281 gtcatataga cgtcgctgag atcgaccata ttcagcaccc gaccgcctgc cgccagcact
    38341 tcccctggct cagcaacccg atactgcacg cgtccgtcac gcggggcttt cagttcactg
    38401 tcatcgatat ctgcggcaat acgccgttca gtggcttgcg cggcttcgac gcgagtttgg
    38461 gcctgaatga tattggtacg ggccgcttct atagccgctt tagaagccga tacctgagct
    38521 tttgccgatt ccagcgcagc tcgggcgctc tcagcggcgg cgcgatcgtc atccagctgt
    38581 tgcgcagaaa tagcccctcg ttgggcgagc gagcgggaac gcgtatgacg tttagccacg
    38641 gagtccagtt cggactggcg ttgattaacc agcgactgcg cggcacgagt ttcgctttgc
    38701 cgttgctcca gcaaagcctg cgcggcagca acggcacttt gtgcttcttt gatttgcgcg
    38761 atggcttcca gtcgctgctc ctgcaacacg cgagtatcca tcttcgccag cacttcgcct
    38821 tcgcgaacaa actggccttc tttcaccaga atggtgtcga tacgcccggc aattttgctg
    38881 gcgatatcca cttccgtcgc ttcaatgcgc ccattgctga cagcaaagcc ttccggcaca
    38941 cccgccgggc gcaacagcca ccacgccacg acggcagcca ccaccagtaa cccgacaacc
    39001 caccacgcca gatggcgctt actcttatcc ataatcgacc cgccataatc cctgcaagca
    39061 aacgacgcgt gttacaactg ccgccgaacg acgttcagcg gattcttcca tccgggataa
    39121 gaaggaaacg acaaatacga ccagcgcgca gagcattgag cgccaacggg gaaggcttag
    39181 tgaaaacggg tagtaatgtt gtcatcatca gcattcctgg ccgtaaatga aacacgcata
    39241 ctcataagtg tgatctataa aactggctga caaaacggta tccatcctgt tggatagcgt
    39301 ttcacgcctg ttcagttgca ggaacagagc attccttttt acgttttatc aacagtctca
    39361 accagatagt aacattaata ttcatgttgt ctatggttca gagctgtaac attgtgcgcc
    39421 ttctgatcaa aggcataagt ccatttctgt ttttttcatg ctggtaagac ttccagggag
    39481 tggttatctc aggccctgca ccagggttaa tttggcaatt atgcatgctg aacattcttc
    39541 cagcatgttt actgtcacgg gcgttaagac ctaaaactgt ccccgtttca cctatacatt
    39601 ctcttccacc attaccgtta tgtgcaacaa cttgatttat acctaacatt tgtgagcatg
    39661 ctaatagtgc ttcaagttta ggtctgtaat ctgtcgaaac agaaaaataa cgattaaagg
    39721 atcgacaaga gatagcccgg ctatttttaa actttttgcc attaattaca gcatggtgct
    39781 ttttattgat aattgccgct aattcgaccg gatcgagagc attggtcatt tcatcactag
    39841 agactgttat cggtcctata atgtaacgtt ttcgctgatc atcaaaagtt atcccatgat
    39901 gatttgccat tattttgtat ttggggtcat agaaacatac aggataatct tttatcccag
    39961 agtaggtatc ctcatcaagt ggtttcatgc gcgtgaagtt ctgcatatag ttaccattgc
    40021 aattcgtttc atgatttcca gctaatacat gaacgttttc attgatactc attatgcgtt
    40081 ttaataaatc gatgataaat ttatcgccac agatattact aaatcgatcg ccagtctggt
    40141 cgcccagaaa cgtacatggc gtgactgcgt ctttattaat aaccaaataa ggctgaagac
    40201 tttctaaaac aatgtctctt ctgttcagga tactgattat tatattaaca ttagtgtcaa
    40261 gttgctcatt aaagttctcc cttagcaata cgttgagaac ttcatcttct atattaagca
    40321 actggctaag gaaattttct ccttcagcca tgattcccaa atgtcccgtt gcaaaaagcg
    40381 cgtacaaaac tgcaccgact gagccatcgg tatcgccaaa ataggtcggt ctggtacttg
    40441 tgggatcaac aggtgaacaa ttggagaaat aggttctgtc agcgaactgg ggttttatca
    40501 ttgtttcgaa tttaatatca aatacattgt attgcgatat tttccagttc cagatgggtt
    40561 tgtacgactc agtaactgca acatcattag cattatcatt tattgtcata ttattaagta
    40621 aataaacaat gctgcaccaa ttaatatcgg tattatcaag agggcaaccg taatttttac
    40681 tattaattgt cacaataaac tgataacagt tattcctacg tagaatatga gcgcgcatgc
    40741 ccgatagcat actatatttt tctgaatgat tcgtttgtat ggtaaatgaa attttgtcat
    40801 cttgaataga ttgttggaac cgttctattt gccaatcatc tattttttcc acaacctcac
    40861 cgagcattgt cctggcaata cttgatatat catcacctat accatttgca atatacccaa
    40921 acagttcttt agtgaagttg gtaacatgct cttgcaccat atcatttgtt gtgattgtcg
    40981 ttgtactgtc gttagtgcct gccacagttc caattttcat cttttacctc atattattta
    41041 ccgcgaggaa tacatagtta acatttaatc ttatgacata cacctcatgt tcgatgggaa
    41101 aataattata ttttgcacaa atcattcagc cttaaagcat aatatgaaat ttcttatctc
    41161 atagcagaga aagtccctaa agattgtaga ggcgtcatca gaacatgcct ctacaatcga
    41221 ttaagaaaac ctatgaaata ctggcaaaat taaaaagaga taccacagca ctcatgaagt
    41281 tttattgagg ttgaaaacaa ctaagccctg ggacaccttt tttatcacca cgttcgttag
    41341 gtgctatctt ccaggaattt agccccatgg tataattatc aattttttga tgctgatcta
    41401 cccctaatcc ataatggcca tatttctgtt tagttttttt gagcgtctgt ccacatttga
    41461 agatatcttc aggttttggt ctgaaagcag tagtgttatt aaaataattt tcatatacag
    41521 gtgtggatgc tacagcattg cttctaatta gatggataag acgatcatcc atgtgttgtt
    41581 tatgtttttt attaaatata tttgctaatt cctcaggatc aattgggtct ttcatctgat
    41641 taaatggaac ctgcaaagca ccgagacaat aacattcatt ttcttcattt cttattattc
    41701 cgtgatgact ggttaaaact ttcgtttctg agtcataatg acaaacatcg agcgttttaa
    41761 ttaagttata cgtatcaccg gcagagagtt tataattttc tagtcgtgca gtatagttac
    41821 cattaaaatt gatttcatga tttccagcca gaacgatgac atttttatta atacggctat
    41881 ctttattacc atccatattt cgcatagaat ttagcaaagt aaggatatat ttatcaccaa
    41941 aaatagtact aaatctatcg cctgtatgat ccccaaggaa gatacaaggg gttatcgcat
    42001 cgtcctttaa aaaaacatgt tgatgcaact cattcaatat catgtctctc gtatttaata
    42061 aattgtagag caatttatta tttttatgta tatatgcaga tgaagccatg ttttcgtgtt
    42121 ttaataattc acataataaa ctccaaccct tttccctgat gcctatatgc cctgaaacga
    42181 gcaatgcata aagtacagcg cctactcttc catctgtatc gccaaaataa gccgtgttgg
    42241 cattttgtgg gttaatcaca ccacattgtt tataaaagtc tattttagaa aacacagatt
    42301 taacatagct atcaaactta tccttaggaa acgtctcttt agagacatcc caacgccagt
    42361 cagctttaaa attaccgcta agaagagtaa agaaattttc aggtatgata gaaagcccat
    42421 taactttata agttatgtct gatttataaa ttatgtctga tttatctccg gagaaattaa
    42481 taaaataata accatcctta cactcaatct taactgtaat gggagacgat aattttatca
    42541 catggttatt gtctggtata attgtaaaga taaacgtgtt agtagtatta tcggcgaaac
    42601 tattatattc ttgaatatct atagatggct cactattgta aaaccatatt ttctccatct
    42661 gaagactatg ttcatttacc ctccagtgac catgttgcag gaaatagatg atattcaggc
    42721 ctaaatcgtt atctatttgc gaaaagtcat tcagcatatt aacttcaact gtgtttgatc
    42781 gatatttttt atcaaccttg ccatggtgtt gatgactgtt aactttatct gaatgcttat
    42841 cttgatggtt tgaagagaag tgcttaaata tattgtgaaa tactttcata aataacctgt
    42901 aatattttta cttgttagta acaaaataaa caatacatgt ctgaaagaca tatttacgat
    42961 gatacaaagc aataatactt atatgttcga tgattttaag caccgagact atccggtctt
    43021 ttactaaata atggaaaaca atccatccct ggaagataat tgtaaaaaaa aacaacagaa
    43081 taagaattaa tcttaggata aatatttatt tatcatggcc ttttaaccgg taaaaaatgg
    43141 cactcggcac agtaaacccc gctatagtta tgctatgcat ttgtctttaa tgatatctcg
    43201 tcgttatatt aacaggctga aattatcatt aatgcacatt tcattattag cagggatacc
    43261 tgaggagttt aaagtgaaca tatatatcgg gtggcttttc aaattaatcc ctttgattat
    43321 gggattaatt tgcatcgcgt taggtggctt tgtgctggaa agttcagggc aaagcgagta
    43381 tttcgtcgcg ggtcatgtgc tgatttctct ggcggccata tgcctggcat tattcactac
    43441 cgcatttatt atcatttcgc agctcacgcg cggcgttaat acgttttaca atacattgtt
    43501 ccccattatt ggctatgcgg ggtcaattat caccatgata tggggttggg cactgttagc
    43561 aggcaatgat gtgatggcag acgagtttgt cgccggccat gttattttcg gcgttggtat
    43621 gattgccgcc tgtgtatcga cggtggcagc gtcatccggt cactttctgc tcattcccaa
    43681 aaatgcagcg gggagcaaga gcgatggaac gccggtacag gcttattctt cattaatcgg
    43741 taattgcctc attgccgttc ccgttttact caccctgctc ggtttcattt ggtctattac
    43801 gctgttacgt agtgctgaca taactccgca ttatgtcgcg ggtcacgtat tgcttgggtt
    43861 aaccgcaatc tgtgcctgtc taattggcct tgttgccaca attgtccatc aaacacgtaa
    43921 tacgttttca actaaagaac actggctgtg gtgttattgg gttatttttc tcggctcaat
    43981 cacggtactg caggggatat acgtcttagt cagttccgat gcaagcgccc gactggctcc
    44041 cggcattatt cttatttgcc tcggaatgat ctgttacagc atattctcaa aagtctggct
    44101 actggcactg gtctggagac gtacctgttc gttagccaac agaataccga tgatacccgt
    44161 cttcacctgc ctgttttgcc ttttcctggc atcatttctt gcggaaatgg cgcagaccga
    44221 catgggatat tttattcctt cgcgagttct ggtcggtttg ggagcggtat gctttacgtt
    44281 gttctcaatc gtttcaatat tagaagcggg ttctgctaaa aaataattgc aacgtaccgg
    44341 ataaaaccag cgttgaccat ttgcgtaacg ctggtttttc ttaggcatca tgaaataacg
    44401 cagcattaat gcatagtggt taaggataaa aaaacaacaa agttactgtc tttttcagcc
    44461 tgttcatatt ttataaagat ctggccactg ggtgagtttt caatcacctt tccttccacc
    44521 ttatattaag cctggagggt ttcagttcac gggctcatta gaaaataatc acaagatagc
    44581 cactatcaaa attagctcat ttttaatgcg gctctaataa ttttcatctt taggtaacag
    44641 gtcaggacga cttcgctgca atcaaatcct gcgcacaagc ccacgcactc gaccacgccc
    44701 actggaagtt atagcccccc agccagccgg taacgtccat cacttcgccg atgaagtaca
    44761 gcccaggcac tttgcgcgct tccatcgtcc gtgaagagag ttcgttggtg tccacgccgc
    44821 cgagcgtcac ttcggcagtg cgatagcctt cagtgccgtt gggttgtacg cgccagtcgg
    44881 tcaatgtgct aatcagtgcc tgttggtcac gcacgttgag ctgttttagc gaaacattcg
    44941 ggatttgccc gagttgctgt aagcgttcaa ccaaccgctt cggtagatga accgccagtg
    45001 tgtttttcag gctctgattc ggatgtgcgt tacgctgctc attcaggaag gtttcgagat
    45061 ccacatccgg tagcagattg atactgacaa attcacccgg ttgccagtag ctggaaatct
    45121 gcaacaccgc cggtccagac aagccgcgat gggtgaagag taagttctca cggaaaacga
    45181 tgccgttttc agcggtaatc acggaaggca ccgccacgcc cgccagcacc tgtaactctt
    45241 cgagcaacgg tttatgcaga gtgaatggca ccagacccgc acgggtcggc agcacgttga
    45301 ggccaaattg ttcggcaatc ttataaccaa acggcgacgc gcccagcccc ggcattgaca
    45361 gcccaccggt cgccatgacc agcttttcgc aaccgacagt catgccgttc agatcaagcg
    45421 tgaagcctgt ttcatcctcc gccacactca gcacttcgct acgcaatctg aaggtcacat
    45481 tgcccttctc gcactcatcc accagcatgt cgacaatctg ctgcgcggag tcatcgcaga
    45541 agagttgccc taacgttttc tcgtgccatg cgatgccgtg tttattgacc agatcaatga
    45601 aatcccactg ggtaaaacgt gcgagtgcag acttacaaaa atgcggattc tggctcagat
    45661 aagcgcctgg ttcgacataa aggttggtaa agttgcagcg cccaccgcca gacataagga
    45721 ttttgcgccc tggtttttta ccattatcga tcagcagaac ccggcgtcct gcctgacctg
    45781 ccagcgcaga acagaacata cccgccgcac cagcgcctat aataatggca tcaaaccttt
    45841 ccacgttgcg ctcctcttag aaaaaacggg cgtgaattgt aaagattcct cagtggtcgc
    45901 accagcatca atattactaa aaggaagtat ttgcctgaat tatataagat aattattttt
    45961 tgagtgaaat ccatacagga ggcaaatcaa aaaaagtcta tatttcactt tgcccgcgcc
    46021 gcgaaagtca ctgataatgc gccgcgttca tgtcctcaaa atggcgtaac gtcctatgct
    46081 acatttgttt gctggcctgg atttgcatac cgggctgtta ttattgcttg cactggcttt
    46141 tgtgctgttc tacgaagcca tcaatggttt ccatgacaca gccaacgccg tggcaaccgt
    46201 tatctatacc cgcgcgatgc gttctcagct cgccgtggtt atggcggcgg tgttcaactt
    46261 tttgggtgtt ttgctgggtg gtctgagtgt tgcctatgcc attgtgcata tgctgccgac
    46321 ggatctgctg cttaatatgg gatcgtctca tggccttgcc atggtgttct ctatgttgct
    46381 ggcggcgatt atctggaacc tgggtacctg gtactttggt ttacctgcat ccagctctca
    46441 tacgctgatt ggcgcgatca tcgggattgg tttaaccaat gcgttgatga ccgggacgtc
    46501 agtggtggat gcactcaata tcccgaaagt attaagtatt ttcggttctc tgatcgtttc
    46561 ccctattgtc ggcctggtgt ttgctggcgg tctgattttc ttgctgcgtc gctactggag
    46621 cggcaccaag aaacgcgccc gtatccacct gaccccagcg gagcgtgaaa agaaagacgg
    46681 caagaaaaag ccgccgttct ggacacgtat cgcgctgatc ctttccgcta tcggcgtggc
    46741 gttttcgcac ggcgcgaacg atggtcagaa aggcattggt ctggttatgt tggtattgat
    46801 tggcgtcgcg ccagcaggct tcgtggtgaa catgaatgcc actggctacg aaatcacccg
    46861 tacccgtgat gccatcaaca acgtcgaagc ttactttgag cagcatcctg cgctgctcaa
    46921 acaggctacc ggtgctgatc agttagtacc ggctccggaa gctggcgcaa cgcaacctgc
    46981 ggagttccac tgccatccgt cgaataccat taacgcgctc aaccgcctga aaggtatgtt
    47041 gaccaccgat gtggaaagct acgacaagct gtcgcttgat caacgtagcc agatgcgccg
    47101 cattatgctg tgcgtttctg acactatcga caaagtggtg aagatgcctg gcgtgagtgc
    47161 tgacgatcag cgcctgttga agaaactgaa gtccgacatg cttagcacca tcgagtatgc
    47221 accggtgtgg atcatcatgg cggtcgcgct ggcgttaggt atcggtacga tgattggctg
    47281 gcgccgtgtg gcaacgacta tcggtgagaa aatcggtaag aaaggcatga cctacgctca
    47341 ggggatgtct gcccagatga cggcggcagt gtctatcggc ctggcgagtt ataccgggat
    47401 gccggtttcc actactcacg tactctcctc ttctgtcgcg gggacgatgg tggtagatgg
    47461 cggcggctta cagcgtaaaa ccgtaaccag tattctgatg gcctgggtgt ttacccttcc
    47521 ggctgcggta ctgctttccg gcgggctgta ctggctctcc ttgcagttcc tgtaatcgta
    47581 cgcaccaaaa cgagcgggtc agctggcccg cttcagattg tgacatagtg cgctttgttc
    47641 atgccggatg cggcgtgaac gccttatccg gcctacaaaa acatgcaaat tcaatagatt
    47701 gcagagatta tgtaggcctg ataagcgtag cgcatcaggc aattttgcgt ttgtaatcag
    47761 tctcgagcgg gtcagtttac tggcccgctt tttttatgcg ttattattgc tgaagtgctt
    47821 aatgccaaat catcaatgca atcaggctga ccaccaccag accacacaat gcgctggtca
    47881 gaataaactg ccgacgcacc cgctcacagc ggcgaataaa ttcatcatcg tgatgatcgc
    47941 gataacgttg ggcatagata taccaaacga gacgcacctg tttgttgggt tggccatgtg
    48001 aggtaaaaaa gccccctcca tcaacatatt gatagagcaa tggatcgcag ttacgcagta
    48061 ccactaacaa cgcgcgtagt gatgagaaat agcgcgccat gttaacaatg caaacgacac
    48121 ataaagccca aaataatgcg acggtgctta tcatacctcc tccccggcga cctgcccgcg
    48181 gagttccacc ccggggctac cgctcccgat acgctgccaa tcagttaaca ccaggtcctg
    48241 gagaaaccgc ttttgtggtg accaacatcc gagcggctct atagatagtg taggagatca
    48301 ggttgttttt tttccagaag gttaaccact atcaatatat tcatgtcgaa aatttgttta
    48361 tctaacgggt aagcaaggcg gattgacgga tcatccgggt cgctataagg taaggatggt
    48421 cttaacactg aatctttacg gctgggttag ccccgcgcac gtagttcgca ggacgcgggt
    48481 gacgtaacgg cacaagaaac gctagctggc cagtcatcga caactttaat ggaaggagta
    48541 acactatggc ttataaacac attctcatcg cggtcgatct ctccccggaa agcaaagttc
    48601 tggtagagaa agcagtctct atggctcgcc cctacaatgc gaaagtttct ctgatccacg
    48661 tagatgtaaa ctactctgac ctatacaccg ggcttattga tgtgaatctg ggtgatatgc
    48721 agaaacgcat ctctgaagag acacatcatg cgctgaccga gctttccact aatgcaggct
    48781 acccaatcac tgaaaccctg agcggcagcg gcgacctggg ccaggttctg gtcgatgcaa
    48841 tcaagaaata cgatatggat ctggtggttt gtggtcacca ccaggacttc tggagcaaac
    48901 taatgtcttc cgcacgtcag ctgatcaaca ccgttcacgt tgatatgctg attgttccgc
    48961 tgcgcgacga agaagaataa tcatcccccc acgacgtgtt cctgaacgcc cgcatatgcg
    49021 ggcgttttgc tttttggcgc gccttgttac ctgatgcagc gtaaacccct tatctggcct
    49081 acgatctgcg tacgcaatca aaatctcctg ccaatacaac atttaacact atcatatttt
    49141 ccatcattag tgtgatcagc tggttatttt ctgttgtaat agtgtattaa tctattcacc
    49201 gcatcaatat taagaatctc tgacagatgt aaactttttc gcgcgttatc ccttacgcgt
    49261 tcatactttt caggatggta ttggaaggtt aataaatatg aatacaacaa cacccatggg
    49321 gatgctgcag caacctcgcc catttttcat gatctttttt gtcgagttat gggagcgatt
    49381 cggctactac ggcgtgcagg gcgtactggc ggttttcttc gttaaacagc ttggattctc
    49441 gcaagagcag gcttttgtca cttttggtgc ttttgctgcg ctggtctatg gcctcatttc
    49501 cattggcggc tatgtcggcg accacctgct ggggaccaaa cgcaccattg ttctcggagc
    49561 acttgtgctg gcgattggct acttcatgac cggcatgtcg ctacttaagc ctgacctgat
    49621 tttcatcgcc ctggggacta tcgctgtcgg taacggcctg tttaaagcta acccagccag
    49681 cttgctttcg aagtgctatc cgccgaaaga tccgcggctt gatggcgcat tcaccctgtt
    49741 ctatatgtcg atcaatatcg gctcgttgat agcgttatcg ctggcccctg tgatcgctga
    49801 tagatacggt tattcagtca cctacaacct gtgcggggcg gggttaatta tcgcattact
    49861 ggtttacatc gcctgtcgtg gaatggtgaa agacattggt tctgaacccg acttcaagcc
    49921 gatgagcttc agcaaactgt tgtacgtatt acttggcagc gtggtgatga tcttcgtatg
    49981 tgcatggctg atgcacaacg tagaagtcgc caatctggtg ctgattgttc tctccatcgt
    50041 cgtcaccatc atcttctttc gtcaggcatt caagctggat aaaaccgggc gcaataaaat
    50101 gtttgtcgcc tttgtcctga tgctcgaagc ggtggtgttt tacattctct acgcccagat
    50161 gccaacgtcg ctgaacttct ttgccatcaa caacgtgcat catgaaattc tcggcttttc
    50221 cataaacccg gtcagcttcc aggcgcttaa cccgttctgg gtggtactcg ccagcccaat
    50281 actggcaggc atttacacgc atctgggtaa caaaggcaaa gacctctcga tgccgatgaa
    50341 atttactctc ggcatgttta tgtgctcact gggctttttg acggcggcag ctgcgggaat
    50401 gtggtttgcg gatgcacaag ggctgacatc gccatggttt atcgtgctgg tgtatttatt
    50461 ccagagcctg ggtgaattgt ttattagcgc ccttggcctg gcgatgattg ctgccctggt
    50521 gccgcagcat ttgatgggct ttattctcgg gatgtggttc ctgacgcagg ctgccgcatt
    50581 cttgctgggc ggctatgtgg caacatttac cgcagtgccg gacaacatta ccgatccgct
    50641 tgagacgtta cccgtctata ccaacgtgtt tggtaagatt ggcctggtta cgctgggcgt
    50701 tgcagtagtg atgctgttga tggtgccgtg gctgaaacgc atgattgcga cgccggaaag
    50761 ccattaatta ttcttgcaga aagcagggta gcgttatcgc taccctgctt tacttttaca
    50821 cgggcgtacc tgcatagata tcaaagcgat gccctttagt cacaaccgcg tttggcgtgg
    50881 cgacattcgc tagcggtggc gcgtagtccg ggcgctttac taccacgcgt ttggtcgcca
    50941 gcaagcgtgc aggctccagc aatccatcgg catcaagatc cggccccacc agcgactgaa
    51001 agacacgcat ctcttttttc accagcgcgc ttttctgctt atgcgggaac atcgggtcga
    51061 gatagaccac ctgcgggcgt ggggtaatat cggttagcgc cgtcaggctg gaggcgtgaa
    51121 ttaactgcaa acgctcctgc aaccagccgc cgatttccgc atccgcataa ccacgcgcca
    51181 ggccgtcgtc gagcagcgcg gcaacaactg gattacgctc cagcatccgc acgcggcaac
    51241 cgactgaagc cagcacaaag gcatcgcgcc ccagtcctgc agtggcatcc accacatccg
    51301 gcaaataatc gcctttaatg cccaccgctt tcgccaccgc ctcaccgcga ccaccgccga
    51361 atttgcgtcg gtgcgccatc gctccgccaa caaaatcaac aaagatgccg ccaagttttg
    51421 gctcatcacg cttgcgcaat tccagatgtt ccggcgttaa caccagcgcc atcaggttgt
    51481 cttcatcgtg ctccagcccc cagcgggccg ccagaacaga taaggcgccg tctccggcgc
    51541 ctgtttcatc aattaagcag attttcactg aatgatcagc ccttaatgcc gtaatgctcc
    51601 agcatcgcat ccagctgcgg ttcacgacca cggaagcgtt tgaacagatc catcggctct
    51661 tctgaaccgc cacggctcag aatgttgtcg aggaacgact gcccggtttc acggttgaaa
    51721 atgccctctt cctcaaagcg cgagaaagca tctgccgcca gtacgtcagc ccacaggtag
    51781 ctgtagtaac ctgccgcata accaccggcg aaaatatggc tgaaagcgtg cgggaaacgg
    51841 ccccaggacg gagatggcac cacggcaacc agtttcttga tttctgccag agtttcgagg
    51901 atttttgccc cctgatccgg gcggaactcg gcatgaaggc ggaaatcaaa caggccgaac
    51961 tccagctgac gcagaataaa cagcgccgcc tggtagttct tcgccgccag cattttatcc
    52021 agcaactctt tcggcagcgg ttcgccggtt tcatagtgac cagagataaa cgccagcgcc
    52081 tccggctccc agcaccagtt ttccataaac tgactcggca gttcgaccgc atcccacggc
    52141 acaccgctga taccggaaac accagcggtt tcgatgcggg tcagcatatg gtgcaggccg
    52201 tgaccgaact cgtggaacag ggtgatcact tcgtcgtgag tgaacagcgc cggtttacca
    52261 tttaccgggc ggttgaagtt acaagtcaaa tacgcgaccg gtttttgcag agaaccatca
    52321 gctttacgca tctggcctac gcagtcatcc atccacgccc cgccgcgctt gttttcacgg
    52381 gcatacagat cgaggtagaa gctgccgcgc agttcgttat tttcgtcata cagttcgaag
    52441 aaacgtacat ccggatgcca gacatcaacc tctttacgct ctttagcggt gatgccgtaa
    52501 atacgtttca ccacttcaaa caggccgtta accgctttgt tttccgggaa gtacggacgc
    52561 agctgctcgt cgctgatgct gtagaggtgc tgtttctgtt tttcgctgta gtaagcgata
    52621 tcccacggct gcaactcatc gacaccaaat tcggctttag cgaaggcacg cagttgctcc
    52681 agctcttttt cgccttgcgg acgcgcgcgt tttgccagat cggttaagaa atccagcacc
    52741 tgctgcgggt tttctgccat tttagtggca agggatttaa aggcgtagtt ttcaaagccc
    52801 agcagttgcg ccagttcgtg acgcagcgcg aggatctctt ccatcacctt gctgttatcc
    52861 cacttaccgg cgttcgggcc ttgatcggag gcgcgggtgc tgtaagcgcg atacatctct
    52921 tcgcgcagag cctggttgtc gcagtaggtc attaccggca ggtagcttgg gatatccagc
    52981 gtcagcaggt agccttccag ctctttcgct tcggcctggg cttttgccgc agccagcgcg
    53041 ctttctggca tccccgccag ccccgcttcg tcggtaacga gtttggtcca gcccatcgtc
    53101 gcatcgagga cgttgttgct gtactggttg cccagttcag aaagacgggt agcaatttcg
    53161 ccgtaacgct gctgtttctc tttcggcaga cctatgccag agagttcaaa atcacgcagg
    53221 gcgttatcaa ccgctttctt ctgcgccgtg ttcagcgtgg cgtaatgatc gccatcgcgc
    53281 aggtcgcggt acgctttata cagcccttca tgttgcccta cccaggtgct gtattccgac
    53341 agcagcggca gggtttgctc gtaggcttca cgcagttccg ggctattttt caccgagttc
    53401 aggtggctga ccggggagaa gatacgcccc aacacatcgt ccacttccgc caacggctgg
    53461 cagagatttt cccaggtgta cggtgcccct tgcgctacta cgcgcgccac attttcgcgg
    53521 caatcgttca gcgcctgagt cacggctgga acgacatgtt ccgggagaat tttagaaaac
    53581 ggaggcaatt caaagggagt cagtaacgga ttcgtcattc gcgcagtcct ggttaaagag
    53641 gttaaggaag cgctcaacag gcgctttaca taatgtgtgt agcatggggt taagtgtagt
    53701 gaatttcaat gagaaacgtt acgctttcgc ggcggcggcc tcttttcggt atactgtcct
    53761 gatacgcttt tgtgcgcccc gaatacgggc cgatttttac ttaccggaac acctttaccc
    53821 atgctcagtt atcgccacag ctttcacgct ggcaaccacg ccgacgtcct taaacatacc
    53881 gttcagagcc tgatcatcga gtcgctgaaa gagaaagata aaccgtttct ctatctcgat
    53941 acccacgcag gggccgggcg ttatcagtta ggcagcgaac atgccgagcg taccggcgaa
    54001 tatctcgaag gtatcgcccg tatctggcag caggacgatt tgcccgcaga actggaggcg
    54061 tacatcaatg tggtaaaaca cttcaaccgt agcggtcagt tgcgttacta ccccgggtcg
    54121 ccgttgattg ctcgccagct actgcgcgaa caagacagcc tgcaactgac cgaactgcac
    54181 ccgagcgatt acccgttgct gcgttctgaa tttcagaaag acagccgtgc gcgcgtcgaa
    54241 aaagccgacg gtttccagca gcttaaggcc aaacttccgc cggtttcccg ccgtggttta
    54301 atccttatcg acccgccgta tgaaatgaaa accgactatc aggcggtggt cagcgggata
    54361 gcagaaggtt acaaacgttt cgccactggt acttacgcac tgtggtatcc ggtggtgctg
    54421 cgtcagcaaa ttaagcgcat gatccacgat ctggaagcga ccggtattcg caaaattctg
    54481 caaattgaac tggcggtact gccagacagc gatcgccgtg gcatgaccgc ttccggcatg
    54541 attgtgatta acccgccgtg gaaactggaa cagcagatga ataacgtgct gccgtggctg
    54601 cacagcaaac tggttccggc aggcaccggg cacgccaccg taagctggat cgtgccggag
    54661 taattgcagc cattgctggc acctattacg tctcgcgcta caatcgcggt aatcaacgat
    54721 aaggacactc tgtcatgact aaacactatg attacatcgc catcggcggc ggcagcggcg
    54781 gtatcgcctc catcaaccgc gcggctatgt acggccagaa atgcgcactg attgaagcca
    54841 aagagctggg cggcacctgc gtgaacgtcg gctgcgtgcc gaaaaaagtg atgtggcacg
    54901 cggcgcaaat ccgtgaagcg atccatatgt acggccctga ttatggtttc gataccacca
    54961 tcaacaagtt caactgggaa acgttgatcg ccagccgtac cgcctatatc gaccgtattc
    55021 atacttccta tgaaaacgtg ctcggtaaaa ataacgttga tgtaatcaaa ggctttgccc
    55081 gcttcgttga tgccaaaacg ctggaggtaa acggcgaaac catcacggcc gatcatattc
    55141 tgatcgccac aggcggtcgt ccgagccacc cggatattcc gggcgtggaa tacggtattg
    55201 attctgatgg cttcttcgcc cttcctgctt tgccagagcg cgtggcggtt gttggcgcgg
    55261 gttacatcgc cgttgagctg gcgggcgtga ttaacggcct cggcgcgaaa acgcatctgt
    55321 ttgtgcgtaa acatgcgccg ctgcgcagct tcgacccgat gatttccgaa acgctggtcg
    55381 aagtgatgaa cgccgaaggc ccgcagctgc acaccaacgc catcccgaaa gcggtagtga
    55441 aaaatgccga tggtagcctg acgctggagc tggaagatgg tcgcagtgaa acggtggatt
    55501 gcctgatttg ggcgattggt cgcgagcctg ccaatgacaa catcaaccta gaagccgctg
    55561 gcgttaaaac taacgaaaaa ggctatatcg tcgtcgataa atatcaaaac accaatattg
    55621 aaggtattta cgcggtgggc gataacacgg gtgcagtgga actgacaccg gtagcagttg
    55681 cagcgggtcg ccgtctctct gaacgcctgt ttaataacaa gccggatgag catctggatt
    55741 acagcaacat tccgaccgtg gtcttcagcc atccgccgat tggtactgtt ggtttaacgg
    55801 aaccgcaggc gcgcgagcag tacggcgacg atcaggtgaa agtgtataaa tcctctttca
    55861 ccgcgatgta taccgccgtc accactcacc gccagccgtg ccgcatgaag ctggtgtgcg
    55921 ttgggccaga agagaagatt gtcggtattc acggcattgg ctttggtatg gacgaaatgt
    55981 tgcagggctt cgcggtggcg ctgaagatgg gcgcaaccaa aaaagacttc gacaataccg
    56041 tcgccattca cccaacggcg gcagaagagt tcgtgaccat gcgctaaatg ttaaagggct
    56101 aagagtagtg tgctcttagc ccttaattac gtttccgcta tcagttcaga agctgaagca
    56161 gaaagcggat cagttccagc agcgcaatta acgctccaag aacgatgatt gctttatcaa
    56221 tcacccgttt tctccatgcg atggagtgag aatgcatccg cttactcatc cactgcctgt
    56281 cacggcgcat gtctcattgt tagatgacga ctatctcact ccggccagag catcagttaa
    56341 cggcaccacc cgtacttctg accaggactt tgaaagcgtt tatgcgcatt accagagtga
    56401 aaatgcctca gagctaactg gataatcata cagtacattc aggttataaa accagcatgt
    56461 ccttgcaata gtttcagtat ggtattagca ttgatgcgtt agatgatgac tatctcactc
    56521 cagccagagc caccaactca gggctgaaga gtaaaaaaac cgacgcaaag tcggtttttt
    56581 tacatccgga ttcggacaag gcttaatatg acgatgaccc agtgaaagta tataaatcgt
    56641 cattgcgata tataccgaag tgccccctcc gccagctgaa gaaatcgcta attcttgcaa
    56701 tgttagccac tggctaatag tattgaaccg ttagatgtgg gcattctcac tccagccaga
    56761 gccaccaact caggctgaag agtaaaaaac cgacgcaaag tcggtttttt tacgtcctga
    56821 ttcaaaccgc ctttcaaatg aatagccaac tcaaaattca cacctattac cttcctctgc
    56881 acttacacat tcgttaagtc atatatgttt ttgacttatc cgcttcgaag agagacacta
    56941 cctgccacaa tcaggagcgc aatatgtcat ttctgttacc catccaattg ttcaaaattc
    57001 ttgctgatga aacccgtctg ggcatcgttt tactgctcag cgagctggga gagttatgcg
    57061 tctgcgatct ttgtactgcc ctcgatcagt cgcagcccaa gatctcccgc cacctggcat
    57121 tgcttcgtga aagcgggcta ttgctggacc gcaagcaagg taagtgggtt cattaccgct
    57181 tatcaccgca tattccagca tgggcggcga aaattattga tgaggcctgg cgatgtgaac
    57241 aggaaaaggt tcaggcgatt gtccgcaacc tggctcgaca aaactgttcc ggggacagta
    57301 agaacatttg cagttaaaaa tttagctaaa cacatatgaa ttttcagatg tgttttatcc
    57361 gggaggcatt atgttactgg caggcgctat ctttgtcctg accatcgtat tggttatctg
    57421 gcagccgaaa ggtttaggca tcggctggag tgcaacgctc ggcgcagtac tggcgttagt
    57481 tacgggcgtg gttcatccgg gtgatattcc ggtggtgtgg aatatcgtct ggaacgcgac
    57541 ggctgcgttt atcgccgtca ttatcatcag cctgctgctg gatgagtccg gcttttttga
    57601 atgggcggcg ctgcacgtct cacgctgggg taatggtcgt ggtcgcttgc tgtttacctg
    57661 gattgtcctg ctcggtgctg ccgttgccgc cctgtttgcc aatgatggcg cggcgcttat
    57721 tttgacgccg attgtcatcg ccatgctgct ggctttagga ttcagtaaag gcacgacgct
    57781 ggcgttcgtg atggcggccg gattcattgc cgataccgcc agcctgccgc ttattgtctc
    57841 caacctggtg aatatcgttt ccgctgattt ctttggcctc ggctttcgcg aatacgcctc
    57901 ggtgatggtg ccggtggata tcgccgcgat tgttgccacg ctggtgatgc tacatctcta
    57961 ttttcgcaaa gatattccgc agaactacga tatggcgctg ctgaaatctc ccgcagaagc
    58021 gatcaaagat cctgctacgt tcaaaactgg ctgggttgtt ttactgcttc tgctggtggg
    58081 atttttcgtc ctggaaccgc tcggcattcc ggtgagcgcc attgcagctg tgggcgcgct
    58141 gatattattt gtcgtcgcta aacgcggtca tgcgattaat acgggtaaag tcctgcgcgg
    58201 tgccccctgg cagattgtca tcttctcgct cggcatgtat ctggtggttt atggcctgcg
    58261 caatgccgga ttaacggaat atctttctgg cgtactcaac gtgctggcgg ataacggcct
    58321 gtgggccgcg acgctcggca ccggattcct caccgccttc ctctcttcta ttatgaacaa
    58381 tatgccgacg gtactggttg gcgcgttgtc cattgatggc agcacggcat ctggcgttat
    58441 caaagaagcg atggtttatg ccaatgtgat tggctgcgat ttgggaccga aaattacccc
    58501 aattggtagc ctggctacgc tactctggct gcacgtactt tcgcagaaga atatgactat
    58561 cagctgggga tattacttcc gtacagggat tatcatgacc ctgcctgtgc tgtttgtgac
    58621 gctggctgcg ctggcgctac gtctctcttt cactttgtaa tgagatactg atatgagcaa
    58681 cattaccatt tatcacaacc cggcctgcgg cacgtcgcgt aatacgctgg agatgatccg
    58741 caacagcggc acagaaccga ccattatcca ttatctggaa actccgccaa cgcgcgatga
    58801 actggtcaaa ctcattgccg atatggggat ttccgtacgc gcgctgctgc gtaaaaacgt
    58861 cgaaccgtat gaggagctgg gccttgcgga agataaattt actgacgatc ggttaatcga
    58921 ctttatgctt cagcacccga ttctgattaa tcgcccgatt gtggtgacgc cgctgggaac
    58981 tcgcctgtgc cgcccttcag aagtggtgct ggaaattctg ccagatgcgc aaaaaggcgc
    59041 attctccaag gaagatggcg agaaagtggt tgatgaagcg ggtaagcgcc tgaaataaag
    59101 cggcgatatc cactgccgat agttggtaaa gtgcgctttg tttatgtcgg atgcgacgct
    59161 aacgcgtctt atccgaccta caaaaccgtg caaattcaat atatggcatg aaatatgtag
    59221 agcgtagcgc atcaggcaat tttgctattt acgcagcact cctcgcgtaa gaattcaatt
    59281 atcgcttcca gcattgcgta ttgcgaaaca cacatcagcg tccgactctc acggttttgt
    59341 ttgaccagtc ctaccgatac cagtgctgat atatggtggc tgagtgtgga gccaggtatc
    59401 ccaagctgct tttgcaactc accgaccggc aacccttgtt ccccggcttt gacaagatgt
    59461 ttaaatatga acaaacgggt cgggtgacct agctctttca gcgctttagc gacttcttcc
    59521 aactgcatat gacacccctc tgtctggttg atatttcgat aataccagaa atatcttgat
    59581 ctgaatttca tttcgattat tctggaaata tagatttatc caatgaggtt acgtatgagt
    59641 agttggcttg cgatgctgca agatgccgca gagatgtttg tgtttctcgc cgtcgagctt
    59701 tcgttgctgt ttatagtgat tagtgccggt gtcagcctga taagacaaaa ggtgccagac
    59761 cataaaatcc agcagatgat gggggccaga aaagggagag gctatctcct ggctgctctg
    59821 ttgggagccg ttaccccgtt ctgtagttgc tcgacaatac ccatgttacg tggattgttg
    59881 tcagcgaaag ccgggt
//
LOCUS       DAESHI010000011        59097 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.011, whole genome shotgun sequence.
ACCESSION   DAESHI010000011 DAESHI010000000
VERSION     DAESHI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59097
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.011"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(130..1077)
                     /gene="dusC"
                     /locus_tag="KW011_000712"
     CDS             complement(130..1077)
                     /gene="dusC"
                     /locus_tag="KW011_000712"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HBH7659135.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKA
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            1316..1714
                     /locus_tag="KW011_000713"
     CDS             1316..1714
                     /locus_tag="KW011_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBH7659136.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            1711..2406
                     /locus_tag="KW011_000714"
     CDS             1711..2406
                     /locus_tag="KW011_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HBH7659137.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            2536..3420
                     /gene="cdd"
                     /locus_tag="KW011_000715"
     CDS             2536..3420
                     /gene="cdd"
                     /locus_tag="KW011_000715"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBH7659138.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            3570..4289
                     /gene="sanA"
                     /locus_tag="KW011_000716"
     CDS             3570..4289
                     /gene="sanA"
                     /locus_tag="KW011_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HBH7659139.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            4292..4531
                     /locus_tag="KW011_000717"
     CDS             4292..4531
                     /locus_tag="KW011_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HBH7659140.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            4725..5963
                     /gene="preT"
                     /locus_tag="KW011_000718"
                     /pseudo
     CDS             4725..5963
                     /gene="preT"
                     /locus_tag="KW011_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            5957..7192
                     /gene="preA"
                     /locus_tag="KW011_000719"
     CDS             5957..7192
                     /gene="preA"
                     /locus_tag="KW011_000719"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HBH7659141.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(7319..8329)
                     /gene="mglC"
                     /locus_tag="KW011_000720"
     CDS             complement(7319..8329)
                     /gene="mglC"
                     /locus_tag="KW011_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HBH7659142.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(8345..9865)
                     /gene="mglA"
                     /locus_tag="KW011_000721"
     CDS             complement(8345..9865)
                     /gene="mglA"
                     /locus_tag="KW011_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HBH7659143.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKFRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(9926..10924)
                     /gene="mglB"
                     /locus_tag="KW011_000722"
     CDS             complement(9926..10924)
                     /gene="mglB"
                     /locus_tag="KW011_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HBH7659144.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(11204..12244)
                     /gene="galS"
                     /locus_tag="KW011_000723"
                     /pseudo
     CDS             complement(11204..12244)
                     /gene="galS"
                     /locus_tag="KW011_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
     gene            complement(12386..13543)
                     /locus_tag="KW011_000724"
     CDS             complement(12386..13543)
                     /locus_tag="KW011_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HBH7659145.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(13560..14228)
                     /gene="folE"
                     /locus_tag="KW011_000725"
     CDS             complement(13560..14228)
                     /gene="folE"
                     /locus_tag="KW011_000725"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBH7659146.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            14486..15322
                     /gene="yeiG"
                     /locus_tag="KW011_000726"
     CDS             14486..15322
                     /gene="yeiG"
                     /locus_tag="KW011_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HBH7659147.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(15354..17345)
                     /gene="cirA"
                     /locus_tag="KW011_000727"
     CDS             complement(15354..17345)
                     /gene="cirA"
                     /locus_tag="KW011_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HBH7659148.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(17637..19106)
                     /gene="lysP"
                     /locus_tag="KW011_000728"
     CDS             complement(17637..19106)
                     /gene="lysP"
                     /locus_tag="KW011_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HBH7659149.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(19311..20192)
                     /locus_tag="KW011_000729"
     CDS             complement(19311..20192)
                     /locus_tag="KW011_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH7659150.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            20291..21340
                     /locus_tag="KW011_000730"
     CDS             20291..21340
                     /locus_tag="KW011_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HBH7659151.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            21414..22271
                     /gene="nfo"
                     /locus_tag="KW011_000731"
     CDS             21414..22271
                     /gene="nfo"
                     /locus_tag="KW011_000731"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBH7659152.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            22275..23363
                     /locus_tag="KW011_000732"
     CDS             22275..23363
                     /locus_tag="KW011_000732"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBH7659153.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(23419..24669)
                     /locus_tag="KW011_000733"
     CDS             complement(23419..24669)
                     /locus_tag="KW011_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBH7659154.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(24769..25710)
                     /gene="rihB"
                     /locus_tag="KW011_000734"
     CDS             complement(24769..25710)
                     /gene="rihB"
                     /locus_tag="KW011_000734"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HBH7659155.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            25840..26538
                     /gene="yeiL"
                     /locus_tag="KW011_000735"
     CDS             25840..26538
                     /gene="yeiL"
                     /locus_tag="KW011_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HBH7659156.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEIDPENKFSGMMQ"
     gene            complement(26605..27855)
                     /locus_tag="KW011_000736"
     CDS             complement(26605..27855)
                     /locus_tag="KW011_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBH7659157.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(27949..28887)
                     /gene="psuG"
                     /locus_tag="KW011_000737"
     CDS             complement(27949..28887)
                     /gene="psuG"
                     /locus_tag="KW011_000737"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HBH7659158.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(28875..29816)
                     /locus_tag="KW011_000738"
     CDS             complement(28875..29816)
                     /locus_tag="KW011_000738"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HBH7659159.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(30274..31965)
                     /gene="fruA"
                     /locus_tag="KW011_000739"
     CDS             complement(30274..31965)
                     /gene="fruA"
                     /locus_tag="KW011_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HBH7659160.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(31982..32920)
                     /gene="fruK"
                     /locus_tag="KW011_000740"
     CDS             complement(31982..32920)
                     /gene="fruK"
                     /locus_tag="KW011_000740"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBH7659161.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(32920..34050)
                     /gene="fruB"
                     /locus_tag="KW011_000741"
     CDS             complement(32920..34050)
                     /gene="fruB"
                     /locus_tag="KW011_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HBH7659162.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            34418..35599
                     /gene="setB"
                     /locus_tag="KW011_000742"
     CDS             34418..35599
                     /gene="setB"
                     /locus_tag="KW011_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HBH7659163.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(35596..35850)
                     /locus_tag="KW011_000743"
     CDS             complement(35596..35850)
                     /locus_tag="KW011_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBH7659164.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            36005..36577
                     /gene="yeiP"
                     /locus_tag="KW011_000744"
     CDS             36005..36577
                     /gene="yeiP"
                     /locus_tag="KW011_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HBH7659165.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            36800..38266
                     /locus_tag="KW011_000745"
     CDS             36800..38266
                     /locus_tag="KW011_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBH7659166.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            38384..39370
                     /gene="yeiR"
                     /locus_tag="KW011_000746"
     CDS             38384..39370
                     /gene="yeiR"
                     /locus_tag="KW011_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HBH7659167.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            39409..40122
                     /gene="lpxT"
                     /locus_tag="KW011_000747"
     CDS             39409..40122
                     /gene="lpxT"
                     /locus_tag="KW011_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HBH7659168.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            40534..41100
                     /gene="mepS"
                     /locus_tag="KW011_000748"
     CDS             40534..41100
                     /gene="mepS"
                     /locus_tag="KW011_000748"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HBH7659169.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            41281..42837
                     /locus_tag="KW011_000749"
     CDS             41281..42837
                     /locus_tag="KW011_000749"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HBH7659170.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            42919..44733
                     /locus_tag="KW011_000750"
     CDS             42919..44733
                     /locus_tag="KW011_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH7659171.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            44734..45828
                     /locus_tag="KW011_000751"
     CDS             44734..45828
                     /locus_tag="KW011_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HBH7659172.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            45828..46853
                     /locus_tag="KW011_000752"
     CDS             45828..46853
                     /locus_tag="KW011_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH7659173.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            46855..48444
                     /gene="yejF"
                     /locus_tag="KW011_000753"
     CDS             46855..48444
                     /gene="yejF"
                     /locus_tag="KW011_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HBH7659174.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(48448..48792)
                     /locus_tag="KW011_000754"
     CDS             complement(48448..48792)
                     /locus_tag="KW011_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659175.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(49125..50245)
                     /locus_tag="KW011_000755"
                     /pseudo
     CDS             complement(49125..50245)
                     /locus_tag="KW011_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(50273..50968)
                     /gene="rsuA"
                     /locus_tag="KW011_000756"
     CDS             complement(50273..50968)
                     /gene="rsuA"
                     /locus_tag="KW011_000756"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HBH7659176.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            51118..52878
                     /locus_tag="KW011_000757"
     CDS             51118..52878
                     /locus_tag="KW011_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBH7659177.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            53003..53287
                     /gene="rplY"
                     /locus_tag="KW011_000758"
     CDS             53003..53287
                     /gene="rplY"
                     /locus_tag="KW011_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HBH7659178.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(53426..54433)
                     /gene="yejK"
                     /locus_tag="KW011_000759"
     CDS             complement(53426..54433)
                     /gene="yejK"
                     /locus_tag="KW011_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HBH7659179.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            54615..54842
                     /locus_tag="KW011_000760"
     CDS             54615..54842
                     /locus_tag="KW011_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HBH7659180.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            54862..56622
                     /gene="pbgA"
                     /locus_tag="KW011_000761"
     CDS             54862..56622
                     /gene="pbgA"
                     /locus_tag="KW011_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HBH7659181.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            56697..56773
                     /locus_tag="KW011_000762"
     tRNA            56697..56773
                     /locus_tag="KW011_000762"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56731..56733,aa:Pro,seq:ggg)
     gene            complement(56876..59097)
                     /gene="yejO"
                     /locus_tag="KW011_000763"
                     /pseudo
     CDS             complement(56876..59097)
                     /gene="yejO"
                     /locus_tag="KW011_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT        14547 a        15427 c        14414 g        14709 t
ORIGIN      
        1 cctcagccca catccatgtg ggctgccccg gccttcaggg aacgcctcgg caattttgac
       61 gccaccaaac aaccgtgcgg cctattgata aagagctaac acattgtcaa aaaacatcac
      121 tatggttttt tagagtttct cgatatcaat tgcctgaata gcccttgcaa tatcagggga
      181 attattcaac acccgaacat gctgaaataa ttccgttgct tcatcgtatt ctttacgcaa
      241 ataactcaac cactgtttaa tccgcgcaac gtgatataac ccggtatcgc cctgcttttc
      301 cagacgggta tatttttgca gcaaagcaac cacctccggc cacggcattc gcggttcgtt
      361 atattttact acccggctca ggttgggaat attgagcgcc ccgcgaccaa tcatcactgc
      421 gtcgcagccg ctgatcgcaa tgcattgttg cgcgctctgc cagtcccaga tttcgccgtt
      481 ggcaatcacc ggaatattca gccgctggcg aatctcgcca atcgcctgcc agtcaatatg
      541 ctccgcgcgg taaccctgct cttttgtccg tccatgcacc accagctccg tagcaccagc
      601 ctgttgaacc gcatcggcga tttcaaattt cttctcaccg ctgtcccagc ccagacgcac
      661 tttcacgctg acgggcaaat gcgccggtac agcttcacgc atcgcttttg caccctggta
      721 gatgagttca ggatctttga gtaacgtcgc cccgccgccg ctgccgttaa ccgctttcga
      781 cgggcagccg caattgagat ccacgcccca ggaacctaat tccaccgcac gggcggcgtt
      841 ctctgccagc cattgtggga actgacctaa caactgaacg cgcaccagcg taccggatgg
      901 tgtccggctg gcgttttgta gctcagggca aatgcgatga aagactttta ccggcagcag
      961 ttgatccacc acgcggacaa actcggtgat gcacagatcg tagtcgttaa cttcggtcag
     1021 caattctcgc accagagagt caagcacacc ctccatcgga gccagtaaca cacgcatatc
     1081 ttcacccgga aaaaaatgag gcgctatgtt agcgcctatg gtcaacggat taaaggtctg
     1141 caaagaggga ttgctgcaaa ctgtaatccc tctctatgct tgatgtatgc ctgagttcgt
     1201 tgcgcattgc gtaatgccgg ggagcgaaaa aataagcgct gatttcttaa tgtgatcggt
     1261 agcacgtttt acgaattaat tgtatgatga atccatctca tctggggtgt tgattatgag
     1321 taagacactg aacattatct ggcaatattt acgcgctttc gtcctgattt atgcctgcct
     1381 gtatgcaggc attttcattg cttccctgct accggtaacc attccgggca gcatcatcgg
     1441 gatgctgatc ctgtttgtcc tgctggcctt gcaaattctt ccggcaaaat gggtcaatcc
     1501 ggggtgctac gtactgattc gctatatggc gctattgttt gtgccgattg gcgtaggcgt
     1561 catgcaatat tttgatttgc tccgcgcaca gtttggcccg gtagtggttt cctgtgcagt
     1621 cagtacgctg gtggtttttc tggtggtgag ctggagttca caactggtac acggtgaacg
     1681 taaagtcgta ggtcagaaag gatcagaaga atgatggcga atatctggtg gtcattaccg
     1741 ctgacgttga ttgtcttttt tgccgcccgc aaactggcgg cacggtataa gtttcccctg
     1801 cttaacccgt tgctggtagc aatggtggtg atcattcctt ttttaatgct gaccggcatc
     1861 tcttacgaca gctactttaa aggtagcgaa gtgctaaacg atctgctgca accggcggtc
     1921 gtcgcgctgg cctatccttt atatgagcag ctacaccaga tccgcgcgcg ctggaaatcg
     1981 atcatcacca tctgttttat cggcagcgtg gttgcgatgg tgacggggac ttccgtggca
     2041 ttgttgatgg gcgcttcacc ggaaatcgcc gcgtcaatcc tgccgaaatc agtcaccacg
     2101 cctattgcaa tggcggttgg cggcagtatt ggcggtattc cggcaatcag cgcagtttgc
     2161 gtgattttcg tcggcatcct cggcgcggta tttggccata cattgcttaa tgcgatgcgt
     2221 attcgtacca aagctgcgcg cggtctggca atggggacag cctcgcacgc cctcggtacg
     2281 gcgcgctgcg ccgagctgga ttatcaggaa ggtgcattta gttcgctggc gctggtgtta
     2341 tgcgggataa ttacttcgct gatcgcaccg ttccttttcc cgattattct ggcagtaatg
     2401 ggctaaaatt tgcgatgcgt cgcgcatttt tgatgtaagt ttcacgcgtt gcataattaa
     2461 tgagattcag atcacatata aagccataac gggttcgtaa actgttatcc cattacatga
     2521 ttatgaggca acgccatgca tccacgtttt caaaccgctt ttgcccaact tgcggataac
     2581 ttgcaatctg cactggaacc tattctggca gacaagtact tccccgcttt gttgaccggg
     2641 gagcaagtct catcgctgaa gagcgcaacg gggctggacg aagacgcgct ggcattcgca
     2701 ctacttccac tggcggcggc ctgtgcgcgt acgccattgt cgaattttaa tgttggcgca
     2761 attgcgcgcg gtgtgagcgg aacctggtat ttcggtgcca atatggaatt tattggtgca
     2821 acaatgcagc aaaccgttca tgccgaacaa agcgcgatca gccacgcctg gttgagtggt
     2881 gaaaaagcgc ttgcagccat caccgttaac tatacgcctt gtggtcactg ccgtcagttt
     2941 atgaatgaac tgaacagcgg tctggatctg cgtattcatc tgccgggccg cgaggcacac
     3001 gcgctgcgtg actatctgcc ggatgccttt gggccgaaag atctggagat taaaacgctg
     3061 ctgatggacg aacaggatca cggctatgcg ctgacgggtg atgcgctttc tcaggcagcg
     3121 attgcggcgg caaaccgttc gcacatgcct tacagtaagt cgccaagcgg tgtcgcgctg
     3181 gaatgtaaag acggtcgtat tttcagcggc agctacgctg aaaacgccgc attcaacccg
     3241 actctgccac cgttgcaggg ggcgttaatt ctgttgaatc tcaagggtta tgattacccg
     3301 gatatccagc gcgcggttct ggcagaaaaa gccgatgcgc cgttgattca gtgggatgcc
     3361 acctccgcaa cgctgaaagc tctcggctgt cacagtatcg accgcgtgct tctcgcttaa
     3421 gcctggtgcc ggatgcggcg tgaacgcctt gtccggcttg ccagccctct cctggtgtcg
     3481 aaattcccgg caaacagttt gccgtttctt gcgcaaaacc agcgggtaaa gtagcctgat
     3541 ggaaattttc cttagatcga gtctcctgca tgttaaagcg cgtgttcctc agcctgttag
     3601 tcctgatcgg cttgctgctg ttgactgtgc tcggcctcga tcgctggatg agctggaaaa
     3661 ccgcgcctta tatctacgac gaattgcagg atctccccta ccgccaggtc ggcgtggtgc
     3721 tcggcacagc aaaatattat cgtactggcg taattaatca gtattatcgc taccgcattc
     3781 aaggagcgat taatgcctat aacagcggta aggtaaatta tctattactg agcggcgata
     3841 acgcattgca aagttataat gagccgatga ccatgcgcaa agatttaatc gctgctggtg
     3901 tcgacccatc agatattgtt ctcgattacg caggctttcg tacgctggat tccatcgtgc
     3961 gtacacgcaa agtgttcgat actaatgatt tcattattat cacccaacgt ttccactgtg
     4021 agcgggcatt atttattgcg ctgcatatgg ggattcaggc tcagtgttat gccgtaccgt
     4081 caccgaaaga tatgctgtca gtacgtattc gcgaatttgc cgcccgtttc ggtgcgctgg
     4141 ctgaccttta tatttttaga cgtgaaccgc gttttttagg gccgctggtc cctattccgg
     4201 ctatgcacca ggtgccggaa gatgcgcagg ggtatcccgc cgtcacaccc gaacagttac
     4261 ttgaattaca aaagaaacaa ggaaagtagt tatggatgta cagcagtttt ttgtcgttgc
     4321 cgtttttttc cttatcccga tattttgttt ccgcgaagca tggaaaggct ggcgcgcagg
     4381 cgcgattgat aaacgggtta aaaatgcacc ggaaccggtg tatgtctggc gagcaaaaaa
     4441 tcccggactc tttttcgctt atatggtggc atatatcggc ttcggaattt tatctatcgg
     4501 catgattgtt tatcttattt tctatcgtta atattttctc ttttctgtta attattgaga
     4561 ataattatta cttcactcaa taagctgcgg atataacatt ctgaaccgca gctatttgtg
     4621 aatcctttca cagtttaaat ttccccacac gcttagcctt aatatcagca cgcaatattt
     4681 atttagcgtt cgccttaatt actcatagca ttaaggaaga tcacatgccg caacaaaatt
     4741 atctggatga actcactccg gcttttacgc ctttactggc gattaaagaa gcctctcgct
     4801 gtttattatg tcatgacgct ccctgtagtc aggcttgccc ggcacagacc gatccgggga
     4861 aatttattcg ctcaatctac tttcgtaatt ttaaaggcgc tgccgagaca attcgcgaaa
     4921 ataatgccct cggtgccgtt tgtgccagag tgtgcccgac ggagaaatta tgccaaagcg
     4981 gttgtacccg tgccggtgtt gatgctccca ttgatatcgg ccgcttacag cgttttgtta
     5041 ctgattttga acaacaaacc gggatggaaa tttatcagcc cggtactaaa acgctcggca
     5101 aagtcgcgat tattggcgca ggtcctgccg gattgcaggc cagtgtgaca ctgacaaacc
     5161 agggttatga cgtcacgatc tatgagaaag aagcacaacc cggtggctga ttgcgtaacg
     5221 gcattccgca attccggtta ccgcaatcag tactggatgc agagatcgcc cgtattgaaa
     5281 aaatgggcgt gaccattaag tgcaacaacg aagtcggtaa cacactcacc cttgagcagc
     5341 tgaaagcaga aaaccgcgcg gtactggtca ccgtggggtt atcaagcggt tccgggctac
     5401 cgctgtttga gcatagtgac gttgagattg ccgtcgactt cttgcaacgt gcacgacagg
     5461 cgcaaggcga tatcagcatt ccacaaagcg cattaattat cggcggcggt gatgtcgcga
     5521 tggacgtagc cagcacgctg aaagttctcg gctgtcaggc ggtaacttgc gtagcgcgtg
     5581 aagagttaga tgagtttccg gcaagcgaaa aagagtttac cagcgcccgg gagctgggcg
     5641 tttcgatcat cgatggattc acgccagtag ccgtggaagg caataaagtc acgtttaagc
     5701 atgtacggtt atcgggcgaa ctgacgatgg cggcagataa aattattctc gccgtcggtc
     5761 agcatgccag actagatgcc tttgcggagt tagagccgca gcgtaacacc atcaaaacac
     5821 aaaattacca gacccgcgac ccgcaagtct ttgctgctgg cgatattgtt gagggagaca
     5881 aaaccgtggt ctatgccgtg aaaaccggga aagaagccgc cgaggcgatt catcactatt
     5941 tagagggagc ttgctcatgt taacgaaaga tctttcgatt actttttgcg gcgtgaagtt
     6001 tcccaacccg ttctgcctct cttcttcgcc ggtaggcaac tgctatgaga tgtgtgccaa
     6061 agcctacgac acaggttggg gcggtgtggt gtttaaaacg atcggctttt ttatcgccaa
     6121 cgaagtctcg ccgcgttttg atcatctggt gaaagaagat accggtttta tcggcttcaa
     6181 aaatatggag cagattgccg agcatccgct ggaggagaat ctggccgccc tgcgtcggct
     6241 gaaagaagat tacccggaca aagtattgat cgcttcgatc atgggggaaa atgagcagca
     6301 atgggaggag ctggcgcgcc tggtgcaaga agctggcgcg gatatgatcg agtgtaactt
     6361 ctcctgtccg caaatgactt ctcatgcgat gggtagcgat gtcgggcaaa gcccggagct
     6421 ggtagaaaaa tattgtcggg cagtgaaacg gggttccacg ctgccaatgc tggcgaagat
     6481 gacgccgaat atcggtgata tgtgcgaagt ggcgctggcg gcgaagcgcg gcggcgcaga
     6541 tggcattgcg gcgattaaca ccgttaaatc catcaccaat atcgatctta atcagaaaat
     6601 cggtatgccg atcgttaacg gaaaatcaag tatttccgga tattccggta aagcggtaaa
     6661 accgatcgcc ctgcgcttca ttcagcaaat gcgcacccat ccagaactgc gcgatttccc
     6721 aatcagcggt atcggcggca ttgaaacctg ggaggatgcg gcggagtttt tattgctcgg
     6781 cgcagcaacg ttacaggtca ccaccggcat tatgcagtac ggctatcgca tcgtagaaga
     6841 tatggcgagc gggttatcgc attatctcgc cgatcaggga tttgattcgt tgcaggagat
     6901 ggtaggtctg gcgaataaca atattgtccc ggcggaagat ttagaccgca gttatattgt
     6961 ctatccccgt atcaatcttg acaaatgtgt tggctgtgga cgctgttata tttcctgtta
     7021 cgacggcggt caccaggcga tggaatggag cgagaaaacc cgcacaccgc attgtaatac
     7081 cgagaaatgt gtgggttgtc tgctttgtgg tcacgtctgc ccggtgggtt gtattgagct
     7141 cggggaagtg aagtttaaga aaggcgagaa agaacacccg gtaacgttgt agaacccgcg
     7201 tcacatccgg catttggttc ctgttgccgg atgcggcgtg aacgccttat ccggcctaca
     7261 aaaccacaca gatcatgatg ttatgtgcgt ttatgaccga atgcggacca cattcacatc
     7321 atttcttacg cgcgtatttc agtgaatcca gcgctacggc gaagataata atcgcccctt
     7381 tgatgatgta ctgccagtat gggtttacgc cgatatacgt caggccatag ttgatgacgg
     7441 taaaaataat caccccggtc accacgccaa tcaccgtccc cacaccgccg ctgaacgata
     7501 caccgcctac cacgcacgcc gcgatagcat ccagctcata cataaagccg aggttgttgg
     7561 tggcagagcc gatacgtccg gcttctaaca tcccgccaaa ggcatagaac acgccagaca
     7621 acgcgtagat catcagcagg ttcaggccga cgttgacacc agatactttt gccgcttccg
     7681 ggttaccgcc aatggcaaaa atgttcttac cgaagcgggt tttgttccac aacacccaga
     7741 cgaacgccac cgcaatcaac gcgtagaagg tgatgtaaga gagacggaaa ctccccagcg
     7801 cgacaaagcc ctgagcaaag gtagagaagc cactgtcaaa accagaaatt ggcgacgccc
     7861 cgacaaagtc gtaatagagc gagttgatgc catagacgat gatcatcgtg ccgagcgtgg
     7921 taatgaacgg cgtcacgttg agataagcga taatcagacc gttgatcaaa ccgatcaccg
     7981 caccaatggc acagacaatc agaataacca gcgcaatcgg catcgtcgcc atttccggga
     8041 acactttgtt ggcgttatcc atggactgca ataatgtcgc agccaccact gccgccagcc
     8101 ctacctgacg accagcagaa agatcggtcc cctgggtgac aattaaccct gccacaccga
     8161 gcgcgataat aatacgcacc gatgactggg tgagaatatt acttaagttc aacagactta
     8221 aaaatgttgg gtcctggaaa ataataatcg ccagcaaaac taaaagaacg acgtaaatac
     8281 cgccctcttt caggtaagta agaaaacttt tcttatttaa cgcactcatg ggaagcccct
     8341 aatcttaaag gtgcaaagac gcaagacgca gaatttcgtt ttgcgttgtt gtttttgtat
     8401 cgacaattcc ggaaacgaga ccattgctca tgaccagaat acggtctgtt atccctaaca
     8461 actcaggcat ttcagaggag ataataataa tccccttgcc tttcttcgcc agttcggcaa
     8521 ttaactgata aatttcaaac ttcgccccga catcaatacc gcgcgtcggt tcatcgagca
     8581 ttaatatttc tggttgcgtt agtagccagc gaccaataat caccttttgc tgattaccac
     8641 cggagagcga accaatttgc gtccgatgac ccggcgtttt tacccgcatc gaatcaatca
     8701 cccactgggt atcgcttttc atccgcgagt tatccagtaa accaacttta tttttgtagt
     8761 tgcgaatatt ggaaattaac gagttaaaac caatatccag ataggcataa attcccgttg
     8821 agcggcgctc ctcagttacc agtgcaaatc cgtggtttat ggcttcgttg gcattatggt
     8881 tattgatctg tttgccgtgc aacgtaatgg tgccagccga tttctcgcga ataccaaata
     8941 acgtctcaac aatatcggta cgtttcgccc ccaccagccc ggcaataccg aggatctccc
     9001 ctttatgcag atcaaacgag acatcgcgaa tcgacggctg gcgcagtgac gttaggttac
     9061 gcacctcgag gatgacttcg cccggcttgt tttctttgtc aggaaaacgc tgattaagag
     9121 aacgcccaac catcatggcg atgatcttgt ccatcgtcag tcctgccagc ggttcggtgg
     9181 cgatccactg accgtcgcgc aatacggtaa cttcatcaca taactggaag atttcttcca
     9241 ttttatgcga gatataaaca ataccgcagc cgcgctcttt taatttacga ataatagtga
     9301 acagatgatt gacctctttt tcggttaacg aagaagtcgg ttcatccata atcacaattt
     9361 tcgcgttata ggaaaacgct ttggcgattt cgatcatctg catttgcgaa acggataatg
     9421 tgccgacgcg cgcacgcgga tcgatatcaa tatccagttc atcaaaaatc gctttggttt
     9481 cgcggtacat tttgtcctga tcgacaaaca tgcctttggt gggatatcgc cccagccaca
     9541 tgttatccat caccgaacgt tgtaatacca ggtttaactc ctggtgtacc atcgaaatac
     9601 cattttccag tgcctctttg gcggaatgga aatcgatctc tttaccctgg aataaaatgg
     9661 tgccggagtc tttttgataa ataccaaaca ggcattttaa taatgtcgat tttcctgcac
     9721 cgttttcccc cattaatgca tggatagaat gtggccggaa ttttaaatta acgttatcaa
     9781 gtgccttaac accaggaaag gacttgttga taccgctcat ttccaacaag tattccccgg
     9841 aagacggagt cgttgagctg accatataat tttaccttgt tggccataca ataagggcgc
     9901 agtaaacgac tgcgcccaat cagtcttatt tcttgctgaa ttcagccagg ttgtctttat
     9961 ctacgccaac ataaggtacg cggaccactt tgttgtcgat tttccagttg gtgccatcag
    10021 ccgcaccttt gccatcggcc aggtttttcg ccagatcaaa ggtcgctttc gcctggttgt
    10081 tagcatcgtt cagtactgtg cccgccagtg caccggattt caccagcgcc agcgcttctg
    10141 gcagcgcatc gacgccaaac accggaatgc tggacttgtt gtgtgctttc agtgcttcta
    10201 ccgcgcccat tgccatcgca tcgttgttgg cgataaccac ttcgattttg ttggcgttcg
    10261 ggccagacag ccaggcgtcc atcttatctt tcgcctgagc ggtgtcccac attgcggtat
    10321 ctaactgtaa ctgttcagtt ttgatgcctt tatcgttcaa ttctttaatc acgtaagtgg
    10381 tacgtgcttc tgcatccgga tggcccggtt cacctttcag cagtacgaac tgaatctgac
    10441 cgtctttgtt cagatcccaa ccctgattcg ccgcccagtg tttagcaatc aaatcgccct
    10501 gaataatgcc ggactctttg gagtcagtgc caacgtagta ggctttgtcg tagctatcca
    10561 gcgccttacg agacggttct ttgttgaaga aaaccaccgg cacgttttgc ccacgcgctt
    10621 tctcaatcac cgtacccgca gctgccgggt caaccaggtt gattgccagt gccttcaccc
    10681 ctttcgccag caatacgtcg atctgatcgt tctgcttgga ctggtcattc tgagaatcat
    10741 tcatcagcag ctgaacatct ggcgcggctt tcgcatcttg ctcaatagcc ttgcgcacta
    10801 cagacataaa gttatcgtcg tacttataga ttgttacacc aatgcgagta tcagcagcgt
    10861 gtgcagcggc accgaataac atgctggcca tcacagcaga cagggttaac accttcttat
    10921 tcatggtatc tccggttttt cttatgcagg gtagtgcttg agataaatgc tcggcggggc
    10981 agtagagtta atgaagtgtt actgaacgcc gaagctcact tttttaaatt cgttcttcca
    11041 tgcccggtaa cgctccagaa aacggcttta attgttgtta tgacgctatt acctcggtga
    11101 aagtgattat tcactgttac atacgggtta caacgttaaa acggtgcaat catagctatc
    11161 acattgttaa gatactgtga aatcactcac agattgaaag cggttacatc gcctgatttg
    11221 ttgagttagt gatcgccgcc gcattctggc gcgttgcgac agaatggcga cgcactaacg
    11281 tcggcataaa acagtggctg gcacgaggat caatattgcc tgctgccccc tgcaaggcca
    11341 gttcggtggc taatttagcc attgaagcaa tgggataacg cacggtcgtt aattgcgggt
    11401 cggtgtaacg ggcgatggga atatcatcga aaccgatgat tgagagatgt aacggaatcg
    11461 caatgccatt atcttttaat gctgtcagtg cgccagcggc catattgtcg ttataagcaa
    11521 atatagcggt aagttgtaga ttgcgcccca gcagttcaac catcgccgcc tcaccgcccg
    11581 gcatgtccgg cgtaccagtg ccaatccagc tttccggcgg aataatatcc tgctctttca
    11641 acgcactcat ccagcctgct ttacgcatgg cgtcatcttc aatgccgtga ctggaagaaa
    11701 gataaccaat acgttgatga ccgttattca gcaacatgcg cgtcgccatt cgggcaccgc
    11761 tgagattatc caggcaaacg caacgatggg cgtaccccgg cacaacgcgg ttgattaaca
    11821 ccataccggg aatgttatcc ataaattgcg ccagttcatc gtcactcaat gcttttgagt
    11881 gaacaatcaa cgcattacaa cgctggcgaa ttaacacctc aatggcgtga cgctcttttt
    11941 ccgcttcatg atagctattg ccgattagca cgtatttctg atgctgctga gcgactagat
    12001 ccaccgcttt taccagcgcg ccgaaaaacg catcagaaac gtccatcacc accacgccaa
    12061 tggtgtcgct aacctgagtt gccagcgcct gggcattggc gtttggccga taatccagct
    12121 cactcacggc tttcattact gcttcacgcg tgtcggcact gaccagcgtg ctgttattga
    12181 gcacccggga aaccgttgcc acagagacgc cagcctaacg cgctacatca cgaatggtga
    12241 tcatattcac tacctgttat gagattgcag taaatgactg cttgctggcg gctattttgt
    12301 cagcactgaa tacaggactt cgtgaatcga gtcacagcaa tggaaacggt tacagccgtt
    12361 ttgttaacga atgtgatcca gatcgttatc ttgatgtttt agatattgcc gttccggcag
    12421 cacgcaaagt taactgacgc cagagccatt ccaccggccc ctggcggaag taacgcagcc
    12481 agataacaga gaagaggata ttcgccagcc ataccggaat aacaaacgcc agcagctcca
    12541 ggcggtcaaa atgcataaac aaaccgaggt ggtaaaaaag catggtacaa atcagcgttt
    12601 gcaatagata gttggtcagc gccatccgtc cgacgcaggc gatcgcaagc accagcttaa
    12661 agcggctcaa ttgcggccag aagccataaa acagcgacgc atagccaatc gcctgaaacg
    12721 gcgcactcag ttcccgcggc atttgaagta agaaggcaca ccagcgatac gcccagtcca
    12781 gctgccattg cagggcgatg gcaggaaggt taatggtcac cccaatcgcc accagcacaa
    12841 aaccagtacg acgatagtga cgtaagctga actgcccttt cagccagccg ctgcgcatca
    12901 gcgccgcacc aatgagcatc atccctgcca gttgccagcc atactgtgcg cccagtgcca
    12961 gtaaactgtt gccaacacca tcggcacgat tactgatcgc ttcaacgccg ccgtgaagct
    13021 tccagtattt ttcatacaaa atagccgatg catccggcgt ccaggtgcgg ctggtctggc
    13081 tgtcggaaat cagccccaat aacagcaaaa cgccaagccc caccagataa agcatgacgc
    13141 ctgtattaaa caggcttttt accgatggcg catcgcgcac cagccgccag cagattaagc
    13201 ccaccagccc gtaagccagc agaatatcgc cgtcccagaa caataaaccg tgaataaaac
    13261 ccagcaagac taacagcgtt aaccgcgact ggatccagcg tctgccacgg ggcagcaaca
    13321 tttgcaggcc cgcaccaaac agcagcgcaa aaagcgtgag gaatttcacc tggccgatga
    13381 gatcgagaaa tgcccaggtc catgcatcct gcggcgtaat agcgccgtac caggcgggat
    13441 tgagataagc cgcctttggt agcccaaagg cgctgatgtt taatagcagg atccccagaa
    13501 tggcgacgcc gcgaacaaaa tcgagcgtta cgttgcgctc catggttcct gccttttaat
    13561 cagttgtgat gacgcacagc gcgcagaaac tcgtggcgcg tattctgact ggatttgaac
    13621 aatccaccaa gagaggtcgt tgttgtggca ctggttgcat cgcggatgcc acgcgctttc
    13681 acgcagtaat gcaccgcatc aatggagaca gccacgttat tggtgcccag cagcgtttgt
    13741 agcgcaataa gaatttgctg cgtcagacgt tcctgcacct gcggacgctg ggcaaagaac
    13801 tgcacgatac ggttaatttt tgacagaccg atcaccgaat ctttcgggat ataagccacc
    13861 gtcgctttgc catcgatggt aacaaaatgg tgttcacagg tactggtcag agtgatatcg
    13921 cgcacggtga ccatttcatc gaccttcatt ttgttttcaa tgagggtgat tttcgggaag
    13981 ttggcgtaat ccagaccgga gaaaatttca tcgacataca ttttagcgat gcgatgcggc
    14041 gtttccatca aactgtcatc agccaggtcg agattcagca gctgcatgat ttcggtcata
    14101 tgaccagcaa taaggctttt gcgcgtttcg ttatccattt catgcacggg cgggcgcagc
    14161 ggtgtttcca gtcctcgcgc aactaacgct tcatgaacca gggccgcttc tttactgagt
    14221 gatggcattt atgatttctc ctgcaggtgt gacgcctccg ccctgcgtgg gggcaaagtt
    14281 attaagctga tttacagcct gattattgtg cgtgaggcgg cgcacataat ccagtattca
    14341 cagcgataat tattgtaatt gccgctgact ttcatcagca gatgttaaaa catcgttatg
    14401 caaatacgga agtgaaagtt actcacagca cattgaataa acggtatgat gaagaaattg
    14461 caaacaacac aacaaggagc cacgcatgga aatgctcgaa gagcaccgct gttttgaagg
    14521 ctggcagcaa cgctggcgac acgactccag taccttaaac tgcccgatga cgttcagtat
    14581 ctttctccca ccccctcgtg atcacacccc gccaccagtg ctgtactggc tttccggatt
    14641 aacctgcaat gacgagaact tcaccaccaa ggcgggtgcc cagcgcgtgg cggcagaact
    14701 ggggattgta ctggtgatgc cagacaccag cccgcgcggc gaaaaggttg ccaacgacga
    14761 tggctacgat ttaggccagg gcgcaggctt ttatcttaat gccacgcaac cgccgtgggc
    14821 gacgcattac cggatgtatg attatctgcg cgatgaatta ccggcgctgg ttcagtcgca
    14881 atttaatgtc agcgaccgct gcgccattag cggtcactca atgggtggtc acggtgcgct
    14941 gattatggcg ctgaaaaatc cgggtaaata caccagcgtt tcggcctttg cgccaattgt
    15001 gaatccgtgc agcgtcccgt ggggaatcaa agcgtttagc acctatttag gtgaggacaa
    15061 aaatgcatgg ctggaatggg atagttgcgc gctgatgtat gccagtaacg cgcaggatgc
    15121 gatcccgacg cttatcgatc agggcgataa tgatcagttt cttgccgacc agttgcaacc
    15181 tgcggtattg gcagaagccg cgcgccagaa agcgtggccg atgacgctgc gtattcagcc
    15241 gggatatgat cacagttact acttcatcgc ctcttttata gaggatcacc tgcgcttcca
    15301 tgcgcagtat ttactgaagt gaaagtccgc ccggttcgcc gggcatcttc tcatcagaag
    15361 cgataatcca ctgccataaa gtaacgacgt ccgtcttcgt tatagctgta gtcgtcacga
    15421 ctgagatcct tgtcgccaag gttcagcacg cctgcgcgca gtttgacgtc tttagtcacc
    15481 tgccaggccg cgccggtatt ccagatggta taaccgcccg gtgttttagc cgtcgcgctg
    15541 tcggcgcgtt tctgcccggt atagtgacca gaaacataga atgaccagtc ttccagcgcc
    15601 agcggtttcc agtccagcgt accgttagca gtatggaacg gcagatcgga tagcggtttg
    15661 ttttcgccgt tgctgacatc acgaccatcg ttgtaggtgt agttgatcga cagtttccat
    15721 tcatcgttga acggaatttt cagttcggtt tccacgccct gaatacgagc tttgttaacg
    15781 ttgtagtagc taaataccgg tatgcgccgt ccgttagcgc ccgtctcaaa accaacaaag
    15841 ttttggtagc ccggtgcagc gttgacgtca gacgtacggc tgatgctgat acgatctttc
    15901 acatcgttac ggaaaacggt aacgctggat tcaacccctt ccagccagcc ttcttcaccc
    15961 atgtagtaaa gccccagctc ccaactttcg ctggtttctg gtttcagatc cgggctaccc
    16021 acaatcttac atgcgccacg gcaggaattg ctcgtccagt cagggctaag ttgcaacaga
    16081 gaaggtgctt taaatgccgt cgcccagccc cctttcaccg ttacggtgtc ggtggcgtta
    16141 taaaccaggt aggcacgcgg actccagtgt tcaccgtagg tttcgtgatc gtccatacgc
    16201 acgccggtcg tcagcgccag cggctcgaag atccgccatt catcttccac aaacagcgcg
    16261 tactggctgg cagacgtttt ggagctggtt cccccggtca ggttcaccgc atcgctaagt
    16321 ttgtcgtgac gccattcacc gccaaccgtg agaaactgat taatcgccgt cagcggcaac
    16381 gtgtatttgc cgtcgaccgt attgctttcg gaagttatcg ggctgctgtt gccagggttt
    16441 ttgttctcga ctttctcacc gtagtatttc agttcgctgg tgccgtaatc ccaacgccca
    16501 ttatggctga cggagtagtt ctggcgttcc aggcggtttt tgtccagcga gtcggaatca
    16561 cgatcctgac ggtcgaaacc gtatccggca gtaaaatcgt gattttgatt cggtgtccag
    16621 gcaaattcga cattgccgtc gcggctggag aatccttcaa tacgcggcgt ttctccggta
    16681 tcggtggtcg ttgagttttg cgggtcatcc ttttcacgtt ttgccaggct gccgtaagct
    16741 ttcattccca gcacaccatc aattaatggt ccactggtaa agaactgacc gttataggta
    16801 tcaccgcgat cgcgatgttc ctgaatggtg gtatcgacgg taacggtacc cgaccatttc
    16861 tgaccgattt ttttggtgat gatattcact acaccgccga gcgcatcgga accgtacagc
    16921 gacgacatcg ggccacggac cacttcaata cgttcgatgg aatcgaccgg gatccagttc
    16981 agatcgaaat cattgtggcg gaagacggca ttgcgggagt tcacgcgttt accgtcgacg
    17041 agaatcaggg tatagctgct gtccagacca cgaatactaa cgcccttacg gttatcccct
    17101 tcgttcgtca gttgtacgcc aggcacttct ttgaggacat ccttcagatt ctgtaccggt
    17161 tttcgctgca gatcttcctg ggtaatgacg ctgatactgg cgggtgcatc tttaaggttt
    17221 tgttcaacgg aagatgcagt gacaaccatc gtttcgccat cgtcatcgac cgctaacaca
    17281 ggccatacac aagaaatagc ggacaaacac agcccgaccc gtacgaaagg gttcaaccta
    17341 aacattccat atctccatga ggtaactacg aaaataaaat gggtatcgct cacatcttct
    17401 ttacgtcccc ttgcgtacgg cagcatcgcg gtggacttat ttgtatgcag gtgatcatcc
    17461 cagaaagccg gaccagcctc tgatttgtca gccttttttg attgtagcgt aacgataatg
    17521 caaacgataa cagttatcaa tccaaatgtt aaattttata tccgcatgat ttgtcaggga
    17581 aatggaattg aaaaagccct ctcggttgag agggcttagc aaggaaggga ggaaacttat
    17641 ttcttatcgt tctgcgggaa cttcatttcg ctgtagcgta cgaagtgagt tcctttaatc
    17701 agcttgtagc cgaaccaaat aatcaggaac agcgggatac caatatacgt tgccgctacg
    17761 ccgccccagt caatggtatc tttcaggaac gcttcgtagt tctggcccaa agtgataatc
    17821 agacacagaa tgaatgcgaa gatcggcccc agtgggaaga aacctgaacg gtacggcaga
    17881 tcgttaatgt cgtgtccctg caatacgtaa ccgcgacgga agcgatagtg gctaatggca
    17941 atccccagcc aggcgataaa acccgtcatc ccggaggtgt tcagcagcca caggtatacc
    18001 gtctggttgc caaacatgga ggtcaggaag cacaaaccgg caatcaccgt cgtcgcatac
    18061 agcgcattac gcggcacgcc accacgcgac aatttagcga aaatgcgcgg cgctttaccg
    18121 tcacacgcca gggtgtacag catacgagta gacgcataca taccggagtt acctgctgac
    18181 agcaccgccg tcagaataac tgcgttcatc accgccgccg cagagagcag acccgcgtgc
    18241 tggaacacca aggtgaacgg actgacgctg atgtctttaa catcgttacg cagcaggctc
    18301 ggatcggtat acggaataat caggctgata atcaggatcg cgaacacata gaacaacagg
    18361 attcgccaga atacctgacg taccgcgcgt ggaatgtttt tcgccggatc ttcggactcg
    18421 cctgcagcaa taccgatcag ctcggttccc tggaaagaga agccgacaat catagctacg
    18481 ccgatcatcg ccgcaaaacc accagcaaac ggcgcttcgc cgattgtcca gttgctccag
    18541 cccgcaggct gcgcgccttt gaagataccg ataatcatca gcacgccaac gatgataaag
    18601 acaataactg tcgtgacttt gatcagtgaa aaccagtatt ccgcttcacc aaagccacga
    18661 actgagatgt agttcagcag gaagataacg ccgaggaaca acgcactcca gatccagccc
    18721 ggtgtatccg ggaaccacca gctcatgacc agctgtgctg caaccaagtc aacggcaata
    18781 gtcaccgccc agttgtacca gtagttccag cccagcgcga agccaaagcc ttcttcaaca
    18841 tagttctgac cgtaagtggc aaacgaaccg gaaaccggca tatatgcagc caattcaccg
    18901 agactggtca tcaggaagta aaccatcagg ccaatcagca tatacgagag caatgccccg
    18961 cccggacctg cctgagaaat cgttgcgcca gaggcaacaa aaagacctgt accgatggaa
    19021 ccgccaatgg caatcatcgt caggtgacgc gcctttaatt cacggcgtaa gcccggcgct
    19081 tctgtagttt tagtttcgga acccatacaa aaatgctatc catcttaaaa atgaggcgcg
    19141 attgtagcag acgattggca atccttccgg cagaaataca cggttataag acaccttcat
    19201 gatcgcccag ggattataag taaagcagcc aatctctttt ctggcgaatg catgctgagg
    19261 gcgctgcgtc acgaaatcga cacagcacca gcatgttctt gtacagcacc ttaacgcggt
    19321 acattcgcgg gatcgcaata gtccagaaag cgccgtagcg cgttggaaag gtgtttttgc
    19381 cgatgatgta tacgccacaa cgtacgcatc aggcgcggga gagggaccgc aacttcactt
    19441 aatgtgcctg cctgcaattg atcttcaatc acacgtcgcg acaggcaact aattcccaac
    19501 ccatgacgca ccgcatgttt gattgcctcg gagttaccta attccatcgc catctcaaac
    19561 ttcggtaaat gtgacagcaa cagataatcg acaatctccc gcgtgccgga accgcgttca
    19621 cgcaggatcc acggcgcagc ggccagctgt tctaaggtga ccggaccacg agccaacggc
    19681 gaagtcggcg cggcgaaaac caccagctcg tcttccagcc acggttcaga aatgatttca
    19741 gtgctgtggc acggtccttc aataaagcca atatcaacgc ggaaatccag caccgcttgc
    19801 atcacgtcct ggctgttccc aacgctaagt tcaatcggca actgcggata atcatggcga
    19861 taacgggcga taactgcagg cagaatgtag ttaccgatgg tactactggc atagatacga
    19921 atcgcgccgt tgtcttcgcg aaacagttgt tcgatttcaa ccgcctgttc aagcaatgcc
    19981 aacgcacgcg gatagagcag ccgcccgtgt tcattaacaa ccagtctttt ccccacgcga
    20041 tcaaacagtt gcacgccaag ctgcccttcc aggtcggtca aggctgcgct cactgctgat
    20101 tgcgacaacg ccagcatcac cgacgcctgg gtggttgatc cacttttcaa tacttctgca
    20161 aaaacttcca actgccggag ggtgatgtgc atagtcgctt accacttata aagattaatt
    20221 ataaatatat aatcaatttt atttttaaac cagttagtcg taaccttatg cccggttaaa
    20281 ggagagggtt atgacgaata tcaccttaca gaaacaacat cgtacactgt ggcattttat
    20341 tccggggtta gccctgagtg cagttatcac cggggtcgcc ctgtggggtg gttccattcc
    20401 cgcggttgcg ggtgccgggt ttagtgccct caccctcgca atcttgttgg ggatggtttt
    20461 aggcaacacc atctatccgc acatctggaa aagctgtgac ggtggtgtgc tgtttgccaa
    20521 gcaatattta ttacgtctgg gtatcattct ttatggcttc cgtctgacgt tctcgcaaat
    20581 tgccgatgtc ggtatcagtg ggatcatcat tgacgtgttg acgctgtcca gtaccttcct
    20641 gctggcttgc ttcctggggc agaaagtgtt tggtctggat aagcacacca gctggttgat
    20701 cggtgccggt agcagtatct gtggtgctgc cgcggtactg gcgactgagt cggtagtgaa
    20761 agcggaagcc agtaaagtaa ccgtggctgt tgcaaccgtt gttatcttcg ggaccgtcgc
    20821 gattttcctc tacccggcga tatatccgct gatgtctcaa tggtttagtc cggaaacttt
    20881 cggtatctac attggttcta ctgtgcacga agtggcgcag gtggtggcgg caggtcatgc
    20941 catcagcccg gatgcggaaa acgcagcagt tatttccaaa atgctgcgcg tgatgatgct
    21001 ggctccgttc ctcatcctgc tggcggcgcg tgttaaacag ctgtctgggg cgaacagcgg
    21061 cgagaaaagc aaaatcacta ttccgtggtt tgccatcttg ttcatcgtcg ttgccatctt
    21121 taactcgttc cacctgttac cgcagagcgt ggtgaacatg ctggtaacgc tggatacctt
    21181 cctgctggca atggcgatgg cggcgctggg tctgaccact cacgtcagcg cgctgaaaaa
    21241 agctggggcg aaacctctgc tgatggcact ggtactgttt gcctggctga ttgttggtgg
    21301 tggtgcgatt aactatgtga ttcaaagcgt catcgcataa accactacat cttgctcctg
    21361 ttaacccgct atcattaccg ttttcctcca gcgggtttaa caggagtcct cgcatgaaat
    21421 acattggagc gcacgttagt gctgctggcg gtctggcaaa tgccgcaatt cgcgccgccg
    21481 aaatcgacgc aaccgcgttt gccttgttca ccaaaaacca acgtcagtgg cgtgccgcac
    21541 cgctcacgac gcaaaccatc gatgaattca aagccgcctg tgaaaaatat cactacacat
    21601 cggcgcaaat tcttccccac gacagttatc tgattaacct cggacatccg gtcactgaag
    21661 ctctggaaaa atcgcgcgat gcctttatag atgaaatgca gcgttgcgaa caactggggc
    21721 tttctttgct caacttccac cctggcagcc atctgatgca gatttcagaa gaggattgcc
    21781 ttgcgcgtat tgccgaatcc atcaacattg cgctggataa aactcaaggt gtaacagcgg
    21841 tgattgaaaa caccgccggt cagggcagta acttagggtt taaattcgaa catctcgcgg
    21901 cgattatcga cggcgtggaa gataaatccc gcgtcggcgt ctgcattgat acctgccatg
    21961 ctttcgctgc cgggtatgat ttgcgtactc cagccgaatg cgaaaaaacg ttcgctgatt
    22021 ttgcccgtat tgtcggcttt aagtatctgc gcgggatgca ccttaacgat gcgaaaagta
    22081 cctttggcag ccgcgttgac cgccatcata gcctcggtga aggcaatatc ggtcatgatg
    22141 cgttccgctg gatcatgcaa gacgaccgtt tcgacggcat tccgctgatc cttgaaacca
    22201 tcaacccgga tatctgggca gaagagatcg cctggctgaa agcgcaacaa actgaaaaag
    22261 cggtggcctg atcgatgaac gacagggaaa agcagattct taaaatttta cggcgtaacc
    22321 cgctgattca acagaatgaa atcgccgata ttctgcaaat cagccgctca cgtgttgccg
    22381 ctcatattat ggatttgatg cgcaagggtt tgattaaagg caaaggatat atccttaccg
    22441 agcaggatta ctgtgtggtt gtgggtgcaa tcaatatgga tattcgcgga atggcggata
    22501 tccgttaccc gcaagcagcc tctcatccag gttctgttca ctgttccgca ggcggcgtcg
    22561 gacgtaatat cgcgcataac ctggctctgc ttggtcggga tgtgcattta atatcagcta
    22621 ttggcaacga tttttacggc gagacattac tggaagaaac gcgccgcgcg ggcgtgaacg
    22681 tttcaaactg cattcgtctg catggacaca gcactgcaac ttatctcgct atcgctaata
    22741 agcaggaaga aactatcctg gcaatcaatg acacacatat tctgcaacag ctaacgcctc
    22801 aactgctcaa tacttcacgg gacttgattc gccatgctgg tgtggtgctg gctgattgta
    22861 atctcacacc tgaagccctg gagtgggtct ttaccattgc tgatgaaatt ccgatgtttg
    22921 tcgataccgt ttccgaattt aaagcgaaca aagtaaaaaa ctggtacagc cggatccaca
    22981 ctctgaaacc aacacaaaat gaactggaaa ttttgtgggg gcaaccgatt aaagacgaca
    23041 acgatcgcat cagagctgta aattcgctgc atcaacaagg tgttaaacgc atttttgttt
    23101 atctgaaaga tgaatctgta ttttgcagcg ataaggatgg agaacaattt ttgctgaccg
    23161 cgccagcgca tacgacagta gacagttttg gtgctgacga tggttttatg gcgggcctgg
    23221 tatatagctt tcttgaagga tacagtttcc gcgacagcgc ccgttttgcg gtagcctgcg
    23281 cggcaatttc gcgcgccagc ggcagcttaa acaaccctac cctgtctgcc gataacgcgc
    23341 tttcattagt gccaatggtg taacaatgtt gccggattcg acgcttgacc gcatcttatc
    23401 cggctcataa attgcgcact acgctaaacc aataaagaat cctgcaatag tagcactcat
    23461 caggttagaa agtgtcgccg ccgccagcgc gcgtaaacca agttgggcga tttccggcgc
    23521 acggtgtggc gcaaccgcag aaaacgcccc caccaccacc ccgatagaac caaagttagc
    23581 gaaaccgcac aacgcgaaag aaataatcgc cacggtttta gcatccagag tgccacccgt
    23641 ttgcagatat ggcgagaaat tgagataagc gacaaattca ttgatcgcca gcttctgccc
    23701 aatcaaactc ccggcaagat ttgcatcact ccagtcaacc cccatcaccc acgccaatgg
    23761 ggccaacagg taacctaaaa tcgactccag cgaagcatgt tcaaaaccaa accagccgcc
    23821 aacaccgccg ataataccgt taatcaacgc aattattgca acaaatgcca taacaactgt
    23881 cgctacaccg gcggcgattt tcagcccggt cattgcccct gtcgcagcgg cttcaataat
    23941 gctttttggc ggtgtttcgg tgaaagagag gttattaaaa gaaacctgtg aagattccgt
    24001 agccgggctt aacaggcggg caaacaagat cccgccaggg atcgccatta acgatgccgc
    24061 cagcaaatat tccacaggta cgcccagtgc ggcgtaacca atcattgtcg aaccagcaat
    24121 cgaggccatg ccactacaaa tcgctgtaaa taattcattg cgattcagac gatcgataaa
    24181 gggtttgacg atcgccggaa tttcgttttg cccgaggaaa atggtggtga ccgcgacgaa
    24241 tgactcgatc ttgctgatat ttaaagcttt ctggaagata ccgccgagaa tgcgaattaa
    24301 gatccccatc acaccgatgt agtagagaat actcaccagt gcagtgacga agataattgc
    24361 cggtaatacc ctgaaaccaa agataaatcc tgcgccatca aataaggtat ccattttcgg
    24421 tccgaccaga gaaccgaaga taaatgcgct acctgcgtcg ctgtacgcca tcactttgtg
    24481 gacgccaaaa gcgacttttt ctgcgaccca acgccctggc ggtaaccaaa gcataatccc
    24541 gccaatcacg acctgtaaca ctaacgccgc gccaacggta cgcaggctga tcttcttctt
    24601 gtttactgac agtaaaaacg caattgccag cagtaccacc attcccagaa cacttctcat
    24661 gacatccata gtgattatct cttcatgtca ttttgggcga cggctttctg cccatcagaa
    24721 atagccgggc ctgcatcttg cgatactgtc ccggcttgtt atcaacactt aatgggtttt
    24781 tatgtagccg cgtacgcact cttcgactaa tccccagaac cagtcggtat caatagtgat
    24841 gccgacttta gtattggcgg gcttgccaag aacgcccagc tcgtcgcaga cggtacgccc
    24901 atagcaaggg ccactgttga cgtccacttc gacgtacatc tcctgggttt taatgccatc
    24961 agggttaatc agataaccga tgcaggtggc gtcgtgcacc gggccgccag ccaggccgta
    25021 gttttcgaac tgcgttttga gagtgaagtt catgatgtcg ctgaacatct ctccggcagg
    25081 gccgcctgcc ctttccattc gtgcaattac gtccggggtg caaacggtct ggttggtaag
    25141 atcgaggccc atcatcacta atggaacgcc ggaggtgaac accacgcgtg cggcttccgg
    25201 gtcggcaaag atattgaact cggcagatgg ggtgaagttg cctgtaccgt aagcgccgcc
    25261 cattagcaca atttcacgga ttttgggcag gattgcgggt tgcatacgca ttgccaccgc
    25321 gatatttgaa agcggaccaa ccggcaccag agtgatatcg ccatcgcttg ccataagggt
    25381 atcaatgatg tatttcaccg cgtgggtact ttctgcctgg cgggtcagcg gctcgaatac
    25441 cgggccatcc agtccggttt caccgtgaat attatcggca acgatttgtt gacgcataat
    25501 gggttgcggc atccccgcat aaaccggaac attaatctcc agtttctggc aaacattcag
    25561 gccattaatt aatgttttat caagcgtctg attacccgca acaatggtga tacctaataa
    25621 atcaatcgcc ggatgtttcg ccgccatcat tatagcaata gcatcatcat gacccggatc
    25681 acaatccaga ataatttttc tcttttccat tgtttatttc ctctgtttcc agttgcgtta
    25741 ttttttctac agcaacgaaa tttcgatcgg gccgatgatt gaatcttaac aacagcgtac
    25801 gaatgctaaa tatgagaaat ctcatagcgg ataaacatcg tgaaagaaat ccacaataat
    25861 gatcttaagc agcaattgat gagtgaatcc gcgtttaagg attgcttttt aacggatgtt
    25921 tcagccgata cgcggctgtt tcatttttta gcgcgtgact acattgtgca ggaagggcaa
    25981 cagccgtcct ggctgtttta cctgacgcga ggccgcgcca ggctttacgc cacgctagct
    26041 aatggtcgcg tgtcgctgat cgatttcttt gccgccccct gttttattgg cgagattgag
    26101 ttaatcgaca aagaccatga accgcgtgcg gtgcaggcta ttgaagagtg ttggtgcctt
    26161 gcgctcccta tgaaacatta ccgcccgctg ttattaaacg acacgctatt tttacgaaaa
    26221 ctctgcgtca ccttaagtca taaaaattat cgtaatattg tttctttaac tcagaatcaa
    26281 tcatttccgt tagtgaatcg cctggcagca tttatattac tctcgcagga aggtgatctt
    26341 tatcacgaaa agcatacgca agcggcagag tatttaggcg tttcttatcg acatctttta
    26401 tatgttctcg cgcaattcat tcacgacggt ttattaatta aaagcaagaa agggtatcta
    26461 attaaaaaca gaaagcagtt gtcaggactg gcgctggaga tagacccgga gaataaattc
    26521 tccgggatga tgcagtaaaa attattggca atagcacgat tgccggatgc aacgcttaat
    26581 aaggcgtaca tccggcaatg aaactcaagc taaaccaata aagaacccgg caatggtcgc
    26641 gctcatcagg ttggaaagcg tcgccgccgc cagcgcccgt aaaccaagct gggcgatttc
    26701 cggcgcgcgt tttggcgaaa tagccgaaaa cgctccaacg acaacaccga tagaaccaaa
    26761 gtttgcaaac ccacagaggg cgaacgagat aatggcaatg gtcttgacat ccagcgtgcc
    26821 gctcgtttgc agatagggcg agaaattgag ataagcaaca aactcattaa tcgccagttt
    26881 ctgcccaatc aaacttccgg caagatttgc atcactccag tccaccccca taatccatgc
    26941 caacggggcc agcacatagc cgaaaatgct ttccagagag acattggcaa aaccaaacca
    27001 gccgccaact ccaccaataa tgccgttgat cagcgcaatg atcgctacaa atgccattac
    27061 caccgtcgcc acaccggcgg cgattttcag cccggtcatt gcaccgttcg ccgcagcttc
    27121 aataatgctt tttggcggtg tttcgctaaa cgacagattt tcaaacgtaa cttgcgaagg
    27181 ttccgtcgcc ggactaagaa tacgtgcaaa caggataccg ccaggaatag ccatcagcga
    27241 agcggctaac aaatagtcaa tcggcacgcc cattccggca tagccaatca tcatcgaacc
    27301 cgcaatagac gccatgccgc tacaaatagc cgtaaatagc tcgttgcgat tcatgcgatc
    27361 aataaaaggt ttaacgatcg ccgggatctc attttgcccg aggaaaatag tggtgactgc
    27421 gacaaaagat tcgattttgc tgatgttgag ggctttctga aaaatactgc caagaatacg
    27481 aatcagcagc cccattacgc caatgtagta cagcaagctg ataagcgcgg taacgaaaat
    27541 aatcgccggg agtacgcgaa aggcgaagat aaagcccgca ccgtcaaaca gaacatccat
    27601 tttgggaccg accagcgaac caaaaataaa ggcacttcct gcgtcactgt aggacatcac
    27661 tttatggacg cctaatgccg cctgctctac tgcccatttt cccgggggaa aatagagcat
    27721 gatgccacca atggcaattt gcagcagcag cgcagcgcca accgtgcgca agctgatgct
    27781 ctttttattt accgacaaca caaaggcaat tgccagcaat accaccatcc ccgcaacact
    27841 tctcattata tccataatga ttttcccttc atgccagtaa acccggcgtc agcgccaggt
    27901 tttggtatgt ttgatgagta cgggcgacgg ctttctgccc gccagaaatt aacccacgag
    27961 acgctgatat tctttggcaa tttcgctcgc cagaatggcg ttattaaaga ccagttgaat
    28021 attggatttc aggctatcac cgccggttag ctccgcaaca cgcgccagca ggaacggtgt
    28081 actctctttc ccaataacac cctgttcttc ggcttcagca acggcttgat caattgctgc
    28141 attgatggtt tcttctggca tcgcaaactg ttccgggatc gggttcgcta ccaccaggcc
    28201 gccgttcaaa ccggtttgcc acttcactgc catcgcgcga gcaatttcgc gggcactgtc
    28261 aagacgaatg ctgacttcaa atgggctggt tcggcagaaa aacgcaggca gcgcttttgt
    28321 ctgataacca attaacggta caccgaaggt ttctaaatat tctgttgtta atcctaaatc
    28381 gagaatagat tttgcccccg cacaaacaac agtgacattg gtatttgcca gttcctgtaa
    28441 atcggcagaa atatcgaagg tatgttccgc tccgcgatgc acaccaccaa tacctccggt
    28501 tgcaaaaact ttaatcccag ccagtgctgc aataatcatg gttgatgcaa cggtagtcgc
    28561 gccatttttc cctgcagcaa cgacaaaagg taaatcacga cgactaactt tcgtcacgtt
    28621 atgtccttca cggcccagca attcgatttc ttctttactt aagcccactt tcatcacacc
    28681 gccaataatg gcgatagtgg ctggtactgc gccctgttta cgaattgttt cttctacttc
    28741 aattgcggtt tgggcatttt gtgggaatgg cattccgtga gaaattatgg tcgattccag
    28801 cgcaacaacc ggttttttat tttttaaagc atcctgtact tcagcggata tttgtaataa
    28861 ttcaggggac agagttaatt cagacattct gtgttctcca ctaacgatct aacattggca
    28921 attgataatt cagggttgtt ggtatattca caggcaagtg ccattgacga acatccctgc
    28981 gcaaatcgaa cagaatcgat aaacggcata ccgtctaccc aacaggaggc cagccccgcc
    29041 atcatggcgt cgcctgcccc agtaacattg atgacattcg ttttaatagg ggccgaccac
    29101 ccactttcac cgttgatatc actgtagtaa acgccatccc ctcccatgct cagcaccaag
    29161 cggttcaggc cgtgttgatg aaaccaggcg gcgacctttg cgacatcttc acgtccggaa
    29221 agtgcaattc cgctcaacgt ttcggcttca agacggttag gttttaacgt atggattttg
    29281 ctcagatgat cgcggatttt gacgcatttc catgccgata ccggatcaat aaatacaggc
    29341 gtttccccgc tattttccag aacccagacc agcgcctctt cactaaggtt gcaatccgcg
    29401 acaataacct ttgcaccgcg aataaactcc tgatgctggg caagaaattc tgcagaaata
    29461 cagtcactaa tactcatatc atttatcgcg acaagcattt ctcctgtatt atcgagcagt
    29521 gataaatagc tggatgtatt ctcacccgga acaattaagc atttatcgac ataaacaccg
    29581 gattgattag tttgcgccag taatgattgc ccataaaaat caccacctac tgcacttaaa
    29641 agccaggcat ttttccccag taacgccaga ttatgagcga tattccttcc cacgccacct
    29701 ggggtaaatt taatttttcc gggattcgaa tccgcataat tcaatggatg atgtgaatac
    29761 ccagccacat caatattcgc cgaaccaata attactatgt agtccttttc gcgcatagca
    29821 cgccccctgg caataaattg ccaacgaaga aattgggtaa aatagccatt acagtcaata
    29881 agactattag agcacgtgtt cagatttaaa cacatgttca taataagcat atttaacgta
    29941 atgtaaatcg atcagaaaaa acgaaatcgg ccagatatga aggcggtcac aaagtttgtc
    30001 tatttatgca gcaacaaaag agaaagttgt gatgtgtaac acacaatatg gcaggcaaga
    30061 aaaagggcag agatgtttct gccctccaga ttgttgacaa cgcgcgcttt gttcatcctg
    30121 gatgtggcgt aaacgcctta tccgacctac aaaagcgcaa aaaattcaat atattgcagg
    30181 gttcatgtag gtctgataag catagcgcat caggcaattt tgcgtttgtc atcatcgggg
    30241 cagagatgtc tcatccccga taatgaagat aaattatgcg tttttttcta ctatttgtgt
    30301 ttccgggcgt ttcaggaagg cataggccaa acccgccacc agcgtaccgg caataattgc
    30361 taccaggtaa cccaataccg gcgtaatagc gccagggatc agcagaacaa acagaccacc
    30421 gtgtggtgcc atcagtttcg caccaatcgc cattgagatt gcgccagtca gcgccccacc
    30481 aacgatacag cacggtagca cacgcatcgg atcacgagca gcaaacggaa ttgcaccttc
    30541 cgaaataaag cacagaccaa gtaccagagc ggctttgcca ccttcctgct gcgctttgtc
    30601 gaatttgcga cgcgccacca ttgttgccag acccattgcc agcggtggca ccatacctgc
    30661 cgccataatc gccgccatcg ggccataggt ttgagtactc agcagaccca caccgaatgc
    30721 gtacgctgct ttgtttaccg gaccgcccat gtcagtacac atcatgccac cgaggatcgc
    30781 ccccagcaga accgcattcg cagtccccat ggtctgcagc cagtgagtca acccatcgag
    30841 aatgccagca accggtttac cgattaggta gatcatcgcc agaccgacca ccagactgga
    30901 aattagcgga atgatcagga tcggtttcag cgcctccata ctctgtggca gtttcaattg
    30961 cgtactgatt aacttcgcaa tgtaaccagc caggaagcca gcaattatac cgccaataaa
    31021 gccagaaccg gtgctgaccg ccagcatacc gccaatcaga cccggtgtga ggcccggacg
    31081 atcggcaatg gagaaggcaa tataacctgc cagtaccggc accatcagcg caaaggctga
    31141 accaccacca atctgcatca gcgccgctgc caacgtaccc ggctctttaa acgcttcgat
    31201 accaaaagca aaagaaagcg cgatacacag accacctgca acaaccatcg gcagcatgta
    31261 agagacgccc gtcagcaagt gacggtaagc gcctgcactc tctttcttac cttcagtggt
    31321 cgctgtttga gctttgcccg ctggttcata cggcgttgct tcagcaaccg ctttatccag
    31381 ttcctgcgca gttttcttca gcgccagacc ggtagaggtg cgatacatcg gtttaccagc
    31441 aaatttcgcc agatccactt cgatatctgc cgccacaatc accagatccg ctgctgcgac
    31501 ttcttcaggg gtgattgcat tgcccgcgcc aacagaacca cgggtttcaa ctttcaccca
    31561 ccagccacgt tttttcgctt cggtttcaat ggcttcagcc gccataaagg tgtgtgctac
    31621 gccagtcggg caagcagtca ccgcaactac gcgtttcgga ccgctggcag caaccggtgc
    31681 tgtcgcagcg accggcgcag tgtaaggttt cgcatggcct ttggcttcac tcaggaacag
    31741 ctcagggtgc gcaactgccc gggaaatatc acccagccag acatttttac cgttcagcgc
    31801 gctgtcattc gggatggaat caccgagaac aatcgccatt tctgcgtcgt tcggattgtc
    31861 gatgatttcc agttttgctt ttcgcgccgc cgcgcccagc agggtcttcg ccatataggc
    31921 gcgtgcctga ccgagattag cgtcaataat cagcagcgtt ttcattatgc ctctcctgct
    31981 gtcagttaaa aggttgtaag tcgacgcgcg ccatcattgc ggccaactgc ggacgatcgg
    32041 taatacccac attgctttga cttaccgcca gggctgcaac agctgtcgcc agacgcagtg
    32101 tgtgttcact ggattcacgc atcagcaagc cataaatcag gccaccaacc atagaatccc
    32161 ctgcgccaac ggtgcttacg acatcgactg acggtggttt ggcgatccat tcgccggagg
    32221 cattaaccca aagcgcgcct tcggcaccca gtgaaataac aacatgcgca ataccttgtt
    32281 cacgtagcgc atgtgcagct tcaatcacat ctttcatttc aggcagttta cggcctgccc
    32341 agatttccaa ctcgcggcgg ttaggtttca ccagccacgg tgccgctttc aaacctgcta
    32401 ctaacgcttc acggctacta tcaaagataa tgcaaggaca ctgactacgc aggcgagtca
    32461 tccagtcggt gaacgcttcc gggctgacgc ctgacggtaa gcttccgctg acacagacca
    32521 tatcgaactg accgagccag ctcagagaat cagtcacaaa gcgttcccag tcggcgaggg
    32581 tgacttcaaa acccgagaag ttgaagtcgg tcacttcgcc gtctttttcc gtcagcttaa
    32641 cgttaattcg ggtgcgcccc tgtacaacct ggaaacggtt ggcaatgccc agctcgctga
    32701 acagttgctg aaaaccatcc tgattgtctt tacccaggaa gccgccaacg gtgacatcaa
    32761 ttcccaggtc ttttaatact ttggccacgt tgatgccttt acccgccgca tgcagaccgg
    32821 tggttttcac caggttcact tcgccgcgtt caatttccgg gcagaaacca acaaggtcat
    32881 aagccggatt aagggtgatc gtagcaacac gtctgctcat tatgcgccct ccccaagacc
    32941 agcagcgata gcgtcgccaa tggctttcag cgcctgttca gcatctgcac cctgggcggt
    33001 aaagcgtagg cgatgacctt tcttaacgcc aagtgccaca actttcatca gactacgtcc
    33061 gtttgccggt ttgccggtgc catcaaggtt tgtcacggta atatcactgt taaattgttt
    33121 aatggtattg accagcatgg tacctggacg agcatgcagg ccgtgttcat tgcgcaccac
    33181 aaactccgcg cttaacacgt cgtcggtcgg cgcatcatcg ctggtcagca gcgccagcaa
    33241 cgttgccgca tccgctttca gcaagcggtc agctttattg tcgagcaata aatcagcgag
    33301 acgcttaaga accgcgatgg gctgatcgtc attcatcgcc acactcacca gcatggctgc
    33361 cgtttcgccg tccacatcaa aagcatttgc cgcacggctt accgcaatcg cgctacgcag
    33421 attgccttcg gcgctatcgc tcagccagat accctgtccg agattcagcg gttgttcatt
    33481 gatggctttg gtgacgaaag tggcgtcaac tgcccccacc tctttcagac gcgcagcgtt
    33541 cagcgcctga agagtcagca gatcgctggc gacgatatcc agcgtcagca tttcgttgtc
    33601 gagcttcagc tgctcactct gcttttcgcc catcagtaat gcgcgaagtt cttctgctgt
    33661 tgttgctgac ttcagttgtt cagcaacgga atcatcgctc agtacgtggg tcagctggcg
    33721 tagcaggccc agatgttcat ccgagctggc agcaataccg attgccacgt acgctacctg
    33781 accgtcaccc caggtgacgc cttccgggaa ctgaaatacc tgaacgccgg ttttcagcac
    33841 ctgatcgcgg gtgtcggtag tgccgtgtgg aatagcaata ccattgccga ggaacgttga
    33901 ggtttgctgt tcgcgcgcca gcatgccatt gacgtagcct tctgctacat taccggcctg
    33961 caccagcgcc gcagcgacct ggcgaatcgc ctcttctttg tctccggcct tttcgcccgg
    34021 atggatgtcc tgtacggata actggaacat agttctcctc tcttgctgaa ttgaaacgat
    34081 tcagcctcta tgagaaaaaa agcgccaacc tggcttaggg ttaaagacaa gatcgcgctg
    34141 aaacgtttca agaaagcata atacttctgt ttcagcacgc aaggaaagcc gtaaagttat
    34201 gagcaaaagt ttgatgtgct gcacaatttt ttgtcatttt tcctaattgc tgatgggaaa
    34261 atccgttatc agcaatttca tttcagcagc gtcagcagcc aatcagcaac agtgaaatgg
    34321 cattttgatt attcgtggcg cttttgattt cgctgtctgt ttattttctg acaagcagcg
    34381 taaactccgc gtcttcctct tccagtgatc gacctgcatg cataactccc ccgcagtctc
    34441 cagcgcgaaa tcgtttgacc tgacctcgac ggcgttttta atcgttgcct ttctcaccgg
    34501 tattgcgggt gctctgcaaa ccccgacact cagtattttc cttaccgatg aagtacatgc
    34561 ccgtccggcg atggtgggat tcttctttac cggcagcgct gtcattggga ttctggtaag
    34621 tcagtttctc gctgggcgct ctgataagcg cggcgatcgc aaatcgctga ttgtcttttg
    34681 ctgcctgtta ggcgtgctgg cctgcaccct ttttgcctgg aatcgcaact actttgtttt
    34741 gctattcgtt ggcgtctttc ttagcagctt tggctcgacc gctaacccgc aaatgtttgc
    34801 ccttgcccgt gaacatgccg acaaaaccgg acgtgaggcg gtgatgttca gctctttttt
    34861 acgcgctcag gtttcactgg catgggtcat tggcccaccg ctggcttatg cgttagcgat
    34921 gggtttcagc tttacggtaa tgtatctgag cgcagcggta gcgtttattg tttgcggcgt
    34981 gatggtgtgg ctgtttttac cgtcgatgca aaaagagctt ccgctggcga ccggcacggt
    35041 tgaagcgccg cgccgtaacc gtcgcgatac gctactgctg tttgtcattt gtacattgat
    35101 gtggggctcg aacagcctgt acatcatcaa catgccgcta tttattatca acgaactgca
    35161 tcttcccgag aaactggccg atgtgatgat ggggaccgcc gccgggctgg aaatcccgac
    35221 gatgttgatt gccggatatt tcgccaaacg tctgggtaag cgtttcttaa tgcgcgttgc
    35281 tgccgtgggt ggcgtctgtt tttacgcagg aatgctgatg gcgcattcac ctgtcattct
    35341 gttgggcttg cagctgctaa atgctatttt tattggcatt ctgggcggca tcgggatgct
    35401 ctattttcag gatctgatgc ccggtcaggc gggttcagcc accacgctct ataccaacac
    35461 ttcgcgcgtg ggctggatca tcgcaggatc agtggcgggc atcgtcgccg aaatctggaa
    35521 ttatcacgct gtgttctggt ttgcgatggt gatgattatc gccactctgt tttgcttact
    35581 gcggattaaa gatgtttaag gcgcggtcag catttccaga tcgataagcc aggtcatggc
    35641 ctgctgacgc gagttaccgc acatttcagc gctggcttgt aagcctgcac agactttcgg
    35701 tcgcagtggc gaggtgaaaa ttttacagcg ttgctgttca tcaagctgaa tgcagggcgt
    35761 attggcgggc ttgccatctg gcataccggg aatagggctg gaaattgaag gggcagtgca
    35821 acacgccccg caacccggac ggcattccat acgttgttct cttaaggtta accaccggat
    35881 tgatgagcgc gcacagtacc accttttttg caccagcaaa agtgcgaata ccacttgccc
    35941 gaaaggcccg tcgcgagtac tttgtcgcga tatttttgac attttcgact acaggaattt
    36001 ttcgatgcca agagcgaacg aaattaaaaa aggtatggta ctgaattaca acggcaaact
    36061 gctgctggtt aaggatattg atattcagtc gcccactgcc cgcggtgccg ctacgctgta
    36121 caaaatgcgt ttttctgatg tccgtaccgg gctgaaagta gaagagcgtt tcaaaggtga
    36181 tgatatcgtt gacaccgtga cgctgacccg ccgttacgtt gacttctcct atgtcgatgg
    36241 caacgaatat gtctttatgg ataaagaaga ctataccccg tataccttca ccaaagatca
    36301 gattgaagaa gagttgctgt ttatgccaga gggcggcatg ccggacatgc aggtgctgac
    36361 ctgggacggt caactgctgg cgcttgagct gccgcagacc gttgatctgg aaatcgttga
    36421 aaccgcacca ggcatcaaag gggcatctgc cagcgcccgt aacaaaccgg cgacattgag
    36481 cactggtctg gtgattcagg taccagaata cttaagcccg ggcgaaaaaa ttcgtatcca
    36541 tatcgaagaa cgccgttata tggggcgtgc tgactaactt cagccgcatg cagaaaaggg
    36601 atagctcagg ctgtcccttt tttaatttat tataccaatc ttctattttg cgcttcctga
    36661 acaccttacg ccctcaattt tcactcgttg attgatcgcc ctcacacttc atcgcattaa
    36721 caatccagac caatttcaat tgctgtcata taactttaca ctgtcattgt taatttatcg
    36781 ttactaagac gtgactccta tgaagacaat tgcctccgtt acgctcccgc atcatgtaca
    36841 cgctccacgc tacgatcgcc agcagttgca atcacgtatc gttcattttg gctttggcgc
    36901 ctttcaccgc gctcatcagg cgttactgac cgatcgtgtg ctgaatgccc agggcggcga
    36961 ctgggggatc tgtgaaatca gcttgttcag cggtgatcaa ctgatgagcc agctccgcgc
    37021 acagaaccat ttatataccg tgctggagaa aggtgcggac ggcaatcagg tgataattgt
    37081 cggtgccgtt cacgaatgcc ttaatgcaaa actggattcc ttagcggcaa ttattgagaa
    37141 attttgcgag ccacaggtgg caattgtttc cctgacgatt accgaaaaag gctattgtat
    37201 tgacccggcc accggtgcac tcgacaccag taatccgcgg attattcacg atctacaaac
    37261 ccctgaagaa cctcactccg caccgggtat tctcgtcgaa gcactgaaac gccgccgtga
    37321 gcgcggcctt acaccgttta ccgtgctctc ctgcgacaat attcccgaca atggccatgt
    37381 ggtgaaaaac gcggtgctgg gaatggcaga aaaatgttcg ccagaactcg ccgggtggat
    37441 aaaagaacac gtcagttttc cgggaaccat ggtcgaccgc attgttccgg ctgcaaccga
    37501 cgaatcactg gcagaaatca gccagcatct gggggtaaat gatccctgcg cgattagctg
    37561 cgaaccgttt atccagtggg tggttgaaga taacttcatc gctgggcgtc ctgcctggga
    37621 agtcgcaggt gtacaaatgg tgaatgatgt cctgccatgg gaagagatga aactgcgtat
    37681 gcttaatggc agccactctt ttctcgctta tctgggttac ctctcaggat tcgtccatat
    37741 cagtgattgc atgcaggatc gcgtatttcg ccatgctgcc agaacattaa tgctggatga
    37801 gcaagcgccg acactgcaaa ttaaagatgt cgatttaaca caatatgcgg ataagttaat
    37861 tgcacgtttt gctaatccgt cgctgaaaca taagacctgg caaatcgcga tggatggcag
    37921 ccagaaatta ccgcaacgta tgctggcagg tattcgcata catctggggc gtgaaacgga
    37981 ctggtcgttg ctggcattag gcgttgctgg ctggatgcgt tacgtcagcg gcgttgatga
    38041 tgccggaaat gccattgatg ttcgcgatcc gcttagcgat aaaattcgcg aacttgttgc
    38101 gggcagcagc agtgaacaac gcgtaaccgc cctgctttcc ctgcgtgaag ttttcggtga
    38161 tgatctgcca gataacccgc attttgtgca ggccatcgaa caagcctggc aacaaatcgt
    38221 acaattcggc gcacatcagg cgctattaaa caccctcaaa atttaacgat ttctgcggtt
    38281 aaagcggatg aagctcacct tcgtccgctc tccccttctc ttttctgcct tttttagcca
    38341 ggattaacgc tcagttaact taccagagta ttgcggagcc gttgtgacca ggaccaacct
    38401 catcaccggt tttctcggca gcgggaaaac cacgtcgatt cttcatctgt tagcccataa
    38461 agatcccaac gaaaaatggg cggtactggt taatgaattt ggggaagtcg gaattgatgg
    38521 tgctttgctc gccgatagcg gcgcattgct gaaagagatc cccggcggct gcatgtgctg
    38581 cgttaatggt ttacccatgc aggtagggtt gaatacctta ctgcgtcagg gaaaaccaga
    38641 ccgcttgttg atagagccga ccgggctggg ccatccgaaa cagatcctcg atctgttaac
    38701 cgcaccagtc tatgaactgt ggatagatct gcgcgccacc ttgtgcattc tcgatccacg
    38761 cctgctgctg gacgaaaaaa gcgccagcaa tgaaaacttc cgtgaccagc tggctgccgc
    38821 agacatcatt gtcgccaata aatccgaccg tgcgacgccc gaaagtgagc aagcgctaca
    38881 gcgttggtgg cagcaaaatg gtggcgatcg acaattaatt cacagtgagc atgggaaagt
    38941 tgacggtcat cttctggatt tgccgcgtcg caatttagcc gagttgcccg ccagcgccgc
    39001 gcattctcat cagcatgtcg tgaaaaaagg gttagcagcg ttaagcctgc cagagcatca
    39061 acgctggcgt cgcagtctga acagcgggca aggatatcag gcctgcggct ggatattcga
    39121 cgctgatacg gtattcgaca ccattggcat tctggaatgg gcgcgacttg caccggtgga
    39181 acgcgtcaaa ggcgtgctgc gtattcccga agggctggta cgaatcaacc gtcagggcga
    39241 tgacctgcac attgaaacgc aaaacgttgc gccaccggac agccgtattg agctgatttc
    39301 cagcagcgaa gctgactgga atgctttgca gagcgcgctg ttgaagcttc gtttagcgac
    39361 taccgcgtaa ggttgcctgc gtttttcagt aagataatta gagaaaatat gattaaaaat
    39421 ttgccgcaaa tagtgttgtt gaatattgtc ggcctcgcgc tgtttctttc ctggtatatt
    39481 cccgttaatc atggattctg gttgccgatt gatgcggata ttttttattt ctttaatcag
    39541 aaactggtcg aaagtaaggc ctttttgtgg ctggttgcat tgaccaacaa tcgcgctttc
    39601 gacggttgtt ctctgctggc gatgggaatg ttgatgctga gtttctggct gaaagaaaac
    39661 gcccctggca gacgacgtat cgtgattatg ggtctggtca tgctattaac tgcagtggta
    39721 ttaaaccagc tgggtcaggc attaattcct gtaaaacggg ccagcccaac attgactttt
    39781 accgatatta accgcgtcag cgaactgctc tctgttccca cgaaagatgc ctcacgagat
    39841 agctttcccg gcgatcacgg catgatgctg cttatttttt cggcattcat gtggcgttat
    39901 ttcggcaaag ttgcaggcct tatcgccctt attatttttg tggtttttgc atttcccaga
    39961 gtaatgattg gcgcacactg gtttactgac atcattgtcg gttcgatgac cgtgatattg
    40021 atcggtttgc cctgggtgtt gctgacgcca ttaagtgatc gattaatcac cttttttgac
    40081 aaatcactac cgggaaaaaa caaacatttc caaaacaaat aactcacagt aattaacatc
    40141 atcaggctta tttttatagt gaggataatc ctgatgatgc gcaccgtgct ttcatccatc
    40201 gcgcgcaaaa atcattctct aagtaaatga atggattgca tgcgtttcac tcaatcgtac
    40261 tttaattgac caatcccgct tattaacttt ctgtatcact ttttcttata aaaaatcatg
    40321 taaaaccgct cgccaagacc gcaccaatcg ggtaatctcg aactcgtttt gcctcggcgg
    40381 tagattatcc tcacagcata taattttgtg cgttagtcca cagatttggc cttaaggaat
    40441 tgtttcaaca tgcccaggta attagtctcg tgccgcttgg cattttttta taacgatatt
    40501 tgtcgttaag gacttcaagg gaaaacaaac aacatggtca aatctcaacc gattttgaga
    40561 tatatcttgc gcgggatccc cgcgattgca gtagcggttc tgctttctgc atgtagtgca
    40621 aataacaccg caaagaatat gcatcctgag acacgtgcag tgggtagtga aacatcatca
    40681 ctgcaagctt ctcaggatga atttgaaaac ctggttcgta atgtcgacgt aaaatcgcga
    40741 attatggatc agtatgctga ctggaaaggc gtacgttatc gtctgggcgg cagcactaaa
    40801 aaaggtatcg attgttctgg ttttgtacaa cgtacattcc gtgagcaatt tggcttagaa
    40861 cttccgcgtt cgacttacga acagcaggaa atgggtaaat ctgtttcccg cagtaatttg
    40921 cgtacgggtg atttagttct gttccgtgcc ggttcaacgg gacgccatgt cggtatttat
    40981 atcggcaaca atcagtttgt ccatgcttcc accagcagtg gtgttattat ttccagcatg
    41041 aatgaaccgt actggaagaa gcgttacaac gaagcacgcc gggttctcag ccgcagctaa
    41101 taaaccgttt ggatgcaatc ccttggctat cctgacgagt taactgaaag cactgcttag
    41161 gcagtgcttt tttgttttca ttcatcagag aaaatgatgt ttccgcgtct tgatccaggc
    41221 tatagtccgg tcattgttat cttttaaatg ttgtcgtaat ttcaggaaat taacggaatc
    41281 atgttcatac gcgctcccaa ttctggacgt aagctcctgc ttacctgcat tgtagcaggc
    41341 gtgatgattg cgatactggt tagctgcctt cagtttttag tggcctggca taagcacgaa
    41401 gtcaaatacg acacactgat tatcgacgta caaaagtatc tcgataccta ttttgccgac
    41461 ctgaaatcca ctactgaccg gcttcagccg ctgaccttag atacctgcca gcaggctaac
    41521 cccgaactga ccgctcgcgc ggcgtttagc atgaatgtcc gtacgtttgt gctggtgaaa
    41581 gataaaaaaa cattctgttc atctgcgact ggtgagatgg acattccact caatgaattg
    41641 attccggcgc tcgacattaa taaaaacgtc gatatggcga tcttacccgg cacgccgatg
    41701 gtgccgaaca aacccgcaat cgtcatctgg tatcgcaacc ctttgctgaa aaatagcggt
    41761 gtgtttgccg ctctgaatct caacctgacg ccttctctct tttatagttc acggcaggaa
    41821 gattacgatg gcgtcgccct cattattggc aatactgcgc tatctacctt ttcttcacgt
    41881 ttgatgaacg ttaatgaatt aaccgacatg ccggtccgtg aaactaaaat tgcgggcatt
    41941 cctctgaccg ttcggcttta tgcggatgac tggacatgga acgatgtgtg gtacgcattt
    42001 ttactgggcg gcatgagtgg aactgtcgtt ggcctgctct gctattacct gatgagcgta
    42061 cgtatgcgcc ccggcagaga aatcatgacc gccatcaagc gcgaacaatt ttacgtggcg
    42121 tatcaaccgg tggtggatac acaagctttg cgagtaacgg gcctggaagt actgctacgc
    42181 tggcggcatc ctgtcgcggg agaaattccc ccggatgcct tcattaactt tgccgaatcg
    42241 caaaagatga ttgtgccgct gactcagcac ctgtttgagt taattgcccg cgatgccgca
    42301 gaattagaaa aagtgctgcc agtaggcgtc aaatttggta ttaacattgc gccggaccat
    42361 ctgcacagcg aaagctttaa agcagatatc cagaaactgc tcacttccct gcccgcacac
    42421 catttccaga ttgtgctgga aattaccgag cgcgatatgc tgaaagagca agaagccaca
    42481 caactcttcg cctggctgca ctcggtcggc gtagaaattg ctattgatga cttcggcacc
    42541 gggcacagcg cgcttatcta tcttgagcgt tttacgctcg attatctgaa aattgaccgt
    42601 ggatttatca acgccatcgg tacggaaacg atcacttcac ccgtacttga cgcggtgctg
    42661 acgctggcga aacgcctcaa tatgctgacg gttgctgaag gggtcgaaac gccggaacag
    42721 gcgcgatggc taagcgaacg cggcgttaat ttcatgcaag gctactggat tagtcgccct
    42781 ttaccgctgg acgattttgt ccgctggctg aagaaaccgt atacgccgca gtggtaaggt
    42841 gtgcttacgt cccttattat tcatagtgaa agcatgccgg attgcggcta atgatgagta
    42901 aaaggaaatc cattgcagat gattgtgcgc atactgctgc tgtttatcgc tctgttcacc
    42961 tttggtgtgc aggcgcaggc tatcaaggaa agctatgcct ttgccgtgct gggcgaaccc
    43021 cggtacgcgt ttaatttcaa ccattttgat tatgtgaacc ccgccgcgcc aaaaggtggg
    43081 cagataacgt tgtcagccct cggcaccttc gataatttca accgctatgc actgcgcggc
    43141 aacccgggcg cacgcaccga gcagctgtac gacacgctat ttacgacttc cgatgacgaa
    43201 ccaggcagtt attacccgct gattgctgaa agcgcacgct atgctgacga ttattcctgg
    43261 gtggaggtcg ctattaatcc gcgcgcccgt tttcatgatg gttcgcccat tactgcccgc
    43321 gatgtagagt ttacttttca aaaatttatg accgaaggcg tgccgcaatt tcgtctggtc
    43381 tacaaaggca ccaccgtcaa agccattgca ccgttaaccg tgcgcattga gttagctaaa
    43441 cccagcaaag aagatatgct gagtctgttt tcgctgccga tatttccaga aaagtactgg
    43501 aaggatcaca aacttagcga cccgctcgcc acgcctccgc ttgccagtgg tccgtaccgc
    43561 gttacgtcct ggaaaatggg gcaaaatatt gtctattccc gcgtgaaaga ttactgggca
    43621 gcaaacttac cggtaaaccg tggacgctgg aatttcgaca ccattcgcta cgattattac
    43681 ctcgatgata atgtcgcctt tgaagcgttt aaagcaggtg cctttgattt gcgtatggaa
    43741 aacgacgcta aaaactgggc cacgcgttat accggtaaaa atttcgataa aaaatacatc
    43801 atcaaagatg agcaaaagaa cgaatcagcc caggatacgc gctggctggc gtttaatatc
    43861 caacgtccgg tattcagcga tcgccgggtc cgggaagcaa tcactctcgc ctttgacttt
    43921 gaatggatga acaaggcgtt gttttacaac gcctggagtc gcacgaacag ttattttcag
    43981 aataccgaat acgcggccag aaattacccc gacgccgcgg agctggtgct tctggcacca
    44041 atgaaaaaag atctaccgcc agaagtcttc acacaaatct accagccgcc ggtatctaaa
    44101 ggcgatggct acgatcgtga caacctgtta aaagccgaca aacttctcaa cgaaacgggc
    44161 tgggtgctga agggtcagca acgcgttaat gccacaacgg gtcagccgct cagctttgaa
    44221 ttattgcttc ccgcaagcag caatagtcag tgggtattgc cgttccagca cagcctgcaa
    44281 cggctgggta tcaatatgga cattcgcaag gtggataact cgcaaatcac taaccgcatg
    44341 cgcagtcgcg actatgacat gatgccgcgc ctatggcggg cgatgccgtg gcccagttcc
    44401 gatttacaga tttcctggtc atcggaatat atcaattcca cttataatgc ccccggcgtg
    44461 caaagcccgg ttatcgactc gctgatcaac caaattattg ccgcgcaggg aaataaagaa
    44521 aaattactgc cgttggggcg agcactggat cgcgtattaa cgtggaatta ttacatgctg
    44581 ccaatgtggt acatggcgga agaccgtctc gcctggtggg ataaattctc ccaacccgct
    44641 gtacgccctg tttacagcct gggtatcgat acctggtggt atgacgttaa taaagcggcc
    44701 aaactgccgt cagccagaca acagggagag tagatgggcg cttacctgat tcgccgtctg
    44761 ttgctggtga tcccaacgtt atgggctatt atcactatca actttttcat cgtgcaaatt
    44821 gcgcctggcg gtccggtcga tcaggccatc gccgccattg agtttggtaa tgccggagta
    44881 ttacccggcg caggcggtga aggtgttcgt gccagccatg cgcaaacggg tgtcggcaat
    44941 atcagcgaca gtaattaccg tggcggacgc ggattagacc cagaagtgat cgctgagatc
    45001 actcatcgct acggtttcga taagccgatc cacgaacgtt acttcaaaat gctctgggac
    45061 tacatccgct ttgattttgg tgatagcctg tttcgcagcg cctcggtgct gacgctgatt
    45121 aaagacagtc tgccggtttc catcaccctc ggattgtgga gcacgctgat tatctatctg
    45181 gtgtcgattc cgttaggcat tcgcaaagct gtttataatg ggagtcgctt tgacgtctgg
    45241 agtagcgcat ttatcatcat cggctacgcc attccggcct ttttgtttgc catcctgctg
    45301 attgtcttct tcgcgggcgg cagctatttc gacctgttcc ctctacgcgg cctggtttcc
    45361 gctaactttg attcgctgcc gtggtatcag aaaatcaccg attatctgtg gcatatcacg
    45421 ctaccggtgc tggcgacagt gattggtggc tttgcggcgc tgaccatgct gaccaaaaac
    45481 tcattccttg atgaagtgcg caagcaatac gtggtgaccg cgcgtgcgaa aggggtaagt
    45541 gagaaaaaca ttctctggaa acatgtgttc cgcaacgcca tgctgctggt gattgccggt
    45601 tttccggcga cgtttatcag catgtttttt accggctcgc tgctgattga ggtgatgttt
    45661 tcactcaatg gtcttggctt actgggctac gaagcgaccg tctcgcgcga ttatcctgta
    45721 atgtttggta ccttgtatat tttcaccctg attggcctgc tgctgaatat tgtcagtgat
    45781 atcagctata cgctggttga tccgcgtata gattttgagg gacgttaatg tcgcgactca
    45841 gccccgtcaa tcaggcccgt tgggcgcgtt ttcgtcataa ccgtcgcggc tactggtcgt
    45901 tatggatttt cctcgttttg tttggtttga gtttgtgttc tgaacttatc gctaacgata
    45961 aaccgttgct ggtgcgttat gacggcagtt ggtatttccc gttgttgaaa aactacagcg
    46021 aaagcgattt tggcggcccg ctggcaagtc aggctgatta tcaggacccg tggctgaaac
    46081 aacggctgga aaataacggc tgggtactgt gggcaccgat tcgctttggt gctaccagta
    46141 tcaactttgc taccgataag cccttccctt ctccaccctc ccggcaaaac tggctgggaa
    46201 cggatgccaa cggcggcgat gtgctggcac gcattctcta tggcacgcgg atctcggttc
    46261 tgtttggcct gatgctgact ctctgttcca gcgtgatggg cgtgctggcg ggggcgctac
    46321 aaggctatta cggcggtaaa gtcgatctct ggggacaacg ctttattgaa gtatggtcgg
    46381 ggatgccgac gctgtttttg attattttac tttccagcgt cgtacagcct aacttctggt
    46441 ggctgctggc aattactgtc ttgtttggct ggatgagtct ggtcggcgtg gtgcgggcgg
    46501 agtttttacg tactcgtaat ttcgactaca ttcgtgcggc acaggcgctt ggcgtcagcg
    46561 atcgcagtat catcctgcgt catatgttgc ctaatgccat ggtcgcgacc ctcacctttt
    46621 taccgtttat tttatgtagt tcgataacca ccctgacctc gctcgatttc ctcggcttcg
    46681 gtctaccgct cggttcaccg tcactcggtg aactgctgtt acaagggaaa aataaccttc
    46741 aggccccgtg gcttgggatc accgccttct tgtcggtggc gatattattg tctttgctga
    46801 tctttattgg tgaagccgtc cgcgacgcat ttgatcctaa taaggcggtg tagcatgacg
    46861 caaactctgt tagcgattga aaatttgtcg gtgggttttc gccatcagca aaccgtacgt
    46921 acagtagtca atgatgtttc actacagatt gaggctggcg aaacgctggc gctggtgggt
    46981 gagtcaggtt caggcaaaag cgttaccgcg ctgtcaattt tacgcctgct cccttccccg
    47041 ccggttgaat atctctccgg cgatattcgt tttcatggcg aatcgctgct tcatgccagc
    47101 gatcaaacgt tgcgcggtgt acgcggtaat aagatcgcca tgatttttca ggaaccgatg
    47161 gtgtcgttaa atccattgca taccctggaa aaacagcttt atgaagtgct ttcactccac
    47221 cgcgggatgc gtcgggaagc ggctcgtggc gaaattctta actgccttga tcgcgtcggt
    47281 atccgccagg cggcaaaacg gctgacagat tatccgcatc agctctccgg cggcgaacgc
    47341 cagcgggtga tgattgcgat ggcgctgtta acgcgaccgg aattattaat tgccgatgaa
    47401 ccgaccaccg cactggacgt ctctgtccag gcgcagattt tacagctgtt gcgcgaactg
    47461 caaggcgagc tgaatatggg catgctgttt attactcata acctcagcat tgtcagaaaa
    47521 ctggcccacc gcgtggcggt aatgcaaaac ggtcgctgtg tcgagcaaaa taacgccgct
    47581 acgctatttg cctcacccac tcatccttac acacaaaagc tactcaacag tgaaccgtca
    47641 ggcgatccgg taccgttgcc agaacctgcc tcaacgttgc tggatgttga acagcttcag
    47701 gttgccttcc ccattcgcaa agggattttg aagcgcattg tggatcataa tgtggtggtg
    47761 aaaaacatca gttttacgct acgggcgggt gaaacactgg gtttagtggg cgagtctggt
    47821 tccgggaaaa gcacgacggg actggcgctg ctgcgactga ttacctctca gggcagcatc
    47881 gtctttgacg gtcagccact gcaagattta aatcgccgcc agctgttacc tactcgtcat
    47941 cgtattcagg tggtatttca ggatccaaac tcctcgctca acccacgact caacgttttg
    48001 cagattattg aggaaggctt acgggttcac cagccgacgc tttctgccgc acaacgcgaa
    48061 caacaagtga tagccgtgat gcatgaagtg ggattagatc ctgaaacacg ccaccgttat
    48121 ccggcggagt tctctggtgg tcagcgacaa cgtattgcga ttgccagggc attaattctt
    48181 aagccctcac tgatcatact tgatgaaccg acatcatcac tcgacaaaac ggtacaggcg
    48241 caaatattga cgctattgaa atcattgcaa caaaagcatc aactggccta tttgtttatc
    48301 agccacgatt tgcacgttgt ccgcgcgtta tgtcatcagg ttatcgtact gcgacaaggg
    48361 gaagtagtgg aacaaggacc gtgcgcgcgc gtgtttgccg caccgcagca ggagtatacg
    48421 cgtcagctac tggcgttgag ctgacgctta aaaaggattg tagtttgaaa agggttcggc
    48481 aattgccaca ccaaaatttt tcaatcggca tgttgcagca aactcgtcct ggcgatttac
    48541 aaacaggcac ggctcacctt cacactccag caccgaacat ggaacttcga tgtcgcttaa
    48601 cgcctggctt agtttataca tcaccgacca tgcattatca ccatccgggc taagcaattt
    48661 aagcgccacc aggctgttgt caccgcacgg tccattttgc ggaatcggtt caaccttcga
    48721 acctgcgaaa ccagcagacc agcgaaagtt ctgcggtaga cgatggacga ttgagagttg
    48781 taatgaagtc acttattttc cccggaagca catttacttc acaatttatt tacatcaatt
    48841 ttaacacatc atcaacaatc cgtctttata cagatcgtaa agattgatgc ctcgcctgca
    48901 tggcctctat ggctctgatt taagtaatat ctgcgggcgt ggtcacgttt gcggggctat
    48961 atgtacccgt ttctgcgatc taactcaacc tttttaacta caatgatgtg actttttaca
    49021 taaattgatt ttacataaaa taaacatata tcgggggaat gatagatttg tggttgacat
    49081 gcatcaacaa aggaagcctt ttagcttcct cgttgtgcaa tagatcaccg ttttttcggt
    49141 cgactggcgt acagacagaa gagaatggag ctggttgcgc agaatgcaat tgaccaaatc
    49201 atcggccatg cagagttaaa ggtcgcaaga gaaagcaatg cgccaacaat tgcccctatg
    49261 ccaaaacgga aggttcctgc cagcgaagat gccgttcccg ccatatgggg aaactcatca
    49321 agaatgaccg ccatcgcatt ggatgacacc atagacacgc agcccacaaa cgccgcaacg
    49381 ccaaccacca gcgaccagaa tcccagcccc agcagcgcac tgatgaccat ccacgctgcc
    49441 ataataaatt gtatccacaa ccccgagcgg aacatattta acgcgccaat gcggcggacg
    49501 aagcggctgt taaagatggt catcacgaac agaaaaacaa tgtttagcgc aaagtaataa
    49561 ccaaagtttt ccggtgcaac gtggttgatt tcaatgtaga caaacggtcc ggcgctcagg
    49621 aacgagaaca ttccggcaaa gctgaaaccg ctggccagca tataactcag gacacgctta
    49681 tgacggaaca gcgccgcaaa gttaccaata gtggtacgaa tgtgaaaagg ctggcgagga
    49741 tccagaagat gtaatgccag ctcagccaca ccagcaccca gccgccaact atcggtgcca
    49801 tcagcggtgc aatggtcgtc accagcatga caaacgacat catccgcgag aactcttctt
    49861 tcgggtaaat atcacgcatc aaagcgttga taaccacgct ggccgcagcc gcagccagcc
    49921 cgtggaagaa acgcatcaca atcagctgat cgatggtttg tgccaacgca cacgccaccg
    49981 cggcggcggc aaacaccagc gtaccgccaa gaaccaccgg cttacgcccg aagctgtctg
    50041 ccatcggccc gtagattaac tgccccaacg caaagcccag aatataagta ctcagggtca
    50101 tctgcgtact gcccgccggt acgccaaact gcgctgaaat taccggtagc gcgggcagat
    50161 acatatcaat cgacagcggc atcaacatgg ccagcaggcc aaggataaaa acaatagcaa
    50221 acgacgaatg ctgtcgggtg gtcacaacgg gctcctgaaa gtcattgaaa agttagacga
    50281 cgctggcaat ttcttcttca gttaacggac gatattcacc gggggctaaa tcagcatcca
    50341 gcgtaatgcc gccaatacgt tcacgatgca gctcaaccac atggttaccc acggcggcga
    50401 acatgcgttt cacctgatga taacgccctt cgctgatggt cagacgaacc tgcgttgggg
    50461 taatcacttc cagcaccgcc ggcttagtga gatctttttc gttatgcagc tgcacgcctt
    50521 tagcaaattg ttctgccgta tcgtcagcta cgggtgatac cagcgtcacc agataggtct
    50581 tctcgcaatg atggcgcgga gaagtaatgc ggtgcgacca ctgaccatca tctgtcatta
    50641 gtaccagacc ggtggtatcg atatccagcc gccctgcggc gtgcagtttc cacgctaccg
    50701 gttcatctag aaaatagagc accgttgggt gatcagggtc gtccgtggag caaacataac
    50761 cctgaggctt attgagcatg aagtaacgtg gaccgtgttg ctgcgccagc gggttgccat
    50821 cgtaagcgac atcatgttca ggaagcagtt tgaacgctgc attacggacg atttcgccat
    50881 cgacggtgac acgattgccg cggatttcac gcccggcaat agcacggcta acgccgagtt
    50941 gctgtgcgat aaatttatca agtcgcatgt gtgtgatttt gcctgtaaaa aacggaggtc
    51001 gggtaatgac ccgaatatct gaactgttgt ctgcccagta tagcggtcta ataacatccc
    51061 tcaagggaaa aagatccgtg gcatactatt agcagaataa tctacctacg cgagaccatg
    51121 atttttacac ttcgcccata tcagcaagaa gccgtggatg ccacgctcaa ccattttcgt
    51181 cgtcataaaa cccctgccgt tatcgtgctg cccaccggtg caggtaaaag cctggtgata
    51241 gcggaactgg cacggctggc tcgtggtcgc gtactggtgc tggcacacgt taaagaactg
    51301 gtggcgcaaa accatgcaaa gtatcaggcg ctggggctgg aagccgatat ttttgccgcc
    51361 gggctgaagc gtaaagagag ccacggtaaa gtggtatttg gcagtgtgca gtcggtcgcc
    51421 cgtaatattg atgcctttca gggtgaattt tcgctgttga ttgtcgatga atgtcaccgt
    51481 attggtgacg atgaagagag ccagtatcag caaatcctca ctcacctgac aaaagtgaat
    51541 ccccacttac gcctgctggg gctgactgcc acgccttttc gattgggcaa aggttggatc
    51601 taccagttcc attatcacgg catggtacgc agcgatgaga aagccctttt ccgtgactgc
    51661 atttatgagc tgcccctgcg ttatatgatt aaacacagct atctgacgcc gccagaacga
    51721 ctggatatgc cagtagtgca atatgatttc agccgcttgc aggcacagag caacgggctg
    51781 ttcagcgaag ccgatctcaa ccgtgagctg aaaaaacagc aacgtattac cccgcacatc
    51841 atcagccaga ttatggagtt cgccgaaaag cgtaaaggag tgatgatttt cgccgcgacg
    51901 gtcgaacacg caaaagagat tgtgggatta ctgcctgccg aagatgcagc actgattact
    51961 ggcgacaccc ccggcgctga gcgcgatgtg ttaattgaaa attttaaagc ccagcgtttt
    52021 cgctatctgg tcaacgtcgc ggtactgacc accggatttg acgccccgca cgtcgatctt
    52081 atcgccattc tgcgccctac cgaatcagtg agtctttacc aacaaattgt cgggcgcggt
    52141 ctgcgtctcg ctccgggcaa gactgattgt ttaattcttg attatgcggg taatcctcac
    52201 gatctctacg cgccggaagt tggtacaccg aaaggcaaaa gtgacaacgt tccggtacag
    52261 gttttctgcc ctgcctgcgg ttttgccaac accttttggg ggaaaacgac cgccgacggg
    52321 acattgattg aacactttgg tcgccgctgt cagggatggt ttgaagatga cgacggtcat
    52381 cgcgaacagt gtgacttccg tttccgtttt aaaaattgcc cgcaatgtaa cgcagaaaat
    52441 gatattgccg ctcgccgctg ccgcgaatgt gacacggtac tggttgatcc ggatgatatg
    52501 ttaaaagcgg cgctacgact gaaagatgcg ctggtattac gctgtagcgg catgtctttg
    52561 cagcatgggc acgacgagaa aggcgaatgg ttgaaaatca cctattacga tgaagacggt
    52621 gcggatgtga gtgagcgttt ccgtctgcaa acacctgccc agcgtaccgc cttcgagcag
    52681 ctttttatcc gcccgcatac gcgcacaccg ggcatcccgc tgcgctggat caccgccgcc
    52741 gatatcctcg cccagcaagc cttattgcga cccccggatt ttgtcgtcgc ccgcatgaaa
    52801 ggccagtact ggcaggtgcg tgaaaaagtg ttcgattacg aaggtcgttt tcgtcgggcg
    52861 cacgaattac gcggttaatg gtagttttga ttgatgtata aggcgattga gtatagaatc
    52921 tcacccgctt ttgcatacgc aaagcagatc acttacctgt tgctgggtcg cctgtagcag
    52981 gattaattta agagagaaag aaatgtttac tatcaacgca gaagtacgta aagagcaggg
    53041 taagggtgcg agccgccgcc tgcgtgccgc taacaagttc ccggcaatca tctacggtgg
    53101 caaagaagcg ccgctggcta tcgagctgga tcacgacaaa gtcatgaaca tgcaagctaa
    53161 agctgaattc tacagcgaag ttctgaccat cgttgttgac ggtaaagaaa tcaaagttaa
    53221 agctcaggac gtacagcgtc acccgtacaa accgaagctg cagcacatcg acttcgttcg
    53281 cgcttaattg ctgaataagt tgtaaaaaac cccgctccgg cggggttttt tgtatctgca
    53341 gattatgcct gattacggta ttgctatttt ttgcaggcca gataaggcgt tcacgccgca
    53401 tctggcattc agaacacaaa accgattaat tgccgccaga cgtccggcgc tgcaattggt
    53461 cgcgcaaatt cggcggcgtg cctttaatgg tcagggtatc ggtcgccggc tcccagaaga
    53521 tgcgttcacc taacagcatc gcatcaaagt taatagtcaa gccaccgccg ctaccggcaa
    53581 acttcgtcaa ctgacgcagt gtgctccggt ctgccgggaa gctctcttcc agttcgtagc
    53641 ctttttcagc cgcaaactcc gtgaagctca cttcgcttac gcctgccagc tctttcgaca
    53701 gcgattccag ttcgatctct tcgcctgcct gcagctgctc attacagtag ctataaacct
    53761 gctggcgcac gttctgtcgc tctgctttat ccagttgtgc ttcggcagtg aaatcatcaa
    53821 ccgcttgcaa cagcccacgg ttttgcgctt tcgtgttcaa tccttcgctg gccccgagga
    53881 aatccataaa gaaatccgcc actttgcgcc ctactcgccc tttcaggaaa gtgagatagc
    53941 gggtggactc tggattggtt tcccattcgg ttaaatcgat acgcgcaaca atatccgcat
    54001 gattgatatc aagataatgg gttgggttga tgtcgagatt ttcgttaaca cgcatactgc
    54061 tcaggttgct cagaactgcc accagcagat actccaccgc cagataacgg tagtggcaaa
    54121 acagcacgaa tccaccgtca gcaaaaggat atttcgctaa ttcgtcacgc aggcgaccag
    54181 ttgccgcgcg gctaaatgcc agaaagtcct cttcgccctg acgctgtaag cgcagcgtct
    54241 gcgccagttc actctcttcg ctaaacaagc cgtaagcttt atttttggcg ctatagaccc
    54301 gatgcagttc tgccaccatc tcaacgacgg tttctgtcgg ttccagcaat gaatcgcgca
    54361 acaccagctc aaggttctgc tcatcacgct tgataagctg gtgcagagca atctggttga
    54421 tatccagact catgataaac tctcctttaa gaccgggcgg tattcaacca ccgccggtta
    54481 agcgacgcaa taaaagatgg cggtcatttt acagaaggca gcgtaacgag aatgatttaa
    54541 cgcataaaaa agcagaaaaa aaaccgttgc tacggtaata tgttgccctt tcatcaacta
    54601 actgatttcg atttatgcca caaatttccc gctacagtga tgaacaggtt gaacaattgc
    54661 tcgcagagct gctcaacgta ctggaaaaac ataaggctcc gaccgacctt tccctgatgg
    54721 tgttaggcaa catggtcact aaccttatca acaccagcat tgccccggcc caacgccagg
    54781 caattgccaa ctcttttgcc cgcgccttac agtcctctat caacgaagac aaagcgcact
    54841 aagggaaaca gataacaggt tatggtaact catcgtcagc gctaccgtga aaaagtctcc
    54901 cagatggtca gttgggggca ctggtttgca ctgttcaata ttctgctttc gctcgtcatt
    54961 ggcagccgtt acctgtttat cgccgactgg ccgacaacgc ttgctggtcg catttattcc
    55021 tacgtaagca ttatcggcca tttcagcttc ctggtgttcg ccacctactt gctgatcctc
    55081 ttcccgctga cctttatcgt cggctcccag aggctgatga ggtttttgtc cgtcattctg
    55141 gcaacggcgg gaatgacgct attactgatc gatagcgaag tctttactcg tttccatctc
    55201 catcttaatc ccatcgtctg gcagttggtt atcaacccag acgaaaatga gatggcgcgc
    55261 gactggcagc tgatgttcat cagcgtgccg gttattttat tgcttgaact ggtgtttgcg
    55321 acgtggagct ggcaaaagct gcgcagcctg acgcgtcgtc gacgcttcgc gcgcccgctg
    55381 gccgcattct tatttatcgc ctttatcgcc tcgcatgtgg tgtatatctg ggccgatgcc
    55441 aacttctatc gcccgatcac catgcagcgc gctaacctgc cgctttcgta cccgatgacg
    55501 gcgcgacgtt ttcttgagaa gcatggtctg cttgatgcgc aggagtatca acgccgtctt
    55561 attgagcaag gtaatccaga cgccgtttcc gttcagtatc cgttaagcga actgcgctat
    55621 cgcgatatgg gcaccgggca gaatgtgctg ttgattactg tcgatggcct gaactactca
    55681 cgcttcgaga agcagatgcc tgcgctggca ggttttgctg agcaaaatat ttcgttcacg
    55741 cgccatatga gctccggcaa cactacagac aacggcatct ttggcctgtt ctatggtatc
    55801 tcgccgagct atatggacgg cattctgtcg acccgtacgc ctgcggcatt aattactgcg
    55861 cttaatcagc aaggctatca gctggggtta ttctcatcag atggctttac cagcccgctg
    55921 tatcgccagg cattgttgtc agatttctcg atgccgagcg tacgcaccca atccgacgag
    55981 cagaccgcca cgcagtggat caactggctg ggccactacg cacaagaaga taaccgctgg
    56041 ttctcgtggg tttctttcaa tggcactaac attgacgaca gcaatcagca ggcatttgca
    56101 cggaaatata gccgggcggc aggcaatgtc gatgaccaga tcaaccgcgt gctcaatgca
    56161 ctgcgtgatt ctggcaaact ggacaatacg gttgtgatta tcactgccgg tcggggtatt
    56221 ccgctgagcg aagacgaaga aacctttgac tggtcccacg gtcatctgca ggtgccatta
    56281 gtgattcact ggccaggcac gccggcgcag cgtattaatg cgctgactga tcataccgat
    56341 ctgatgacga cgctgatgca acgcctgcta catgtcagca cacctgccag cgaatattcg
    56401 caaggtcagg atttgttcaa ccctcaacgc cgtcattact gggttactgc agcggataac
    56461 gatacgctgg caattaccac cccgaaaaag acgctggtgc tgaacaataa cggtaaatac
    56521 cgcacttaca acttacgtgg tgaaagagtg aaagatgaaa aaccacagtt aagtttgtta
    56581 ttgcaagtgc tgacagacga gaagcgtttt atcgctaact gattaattat aaatcagtta
    56641 gcgaaatatc ttacttgcaa tcggtgtgga aaacggtagt attagcagcc acgagtcggc
    56701 acgtagcgca gcctggtagc gcaccgtcat ggggtgtcgg gggtcggagg ttcaaatcct
    56761 ctcgtgccga ccaaaaatcc caagaaaaaa ccaaccttta cggttggttt ttttatatct
    56821 gcaattaatt tgataaacag accgtgacac atcacagcct gtttattttc tgttatcaga
    56881 acgtccagac cactcccgcc tgagtattcc acggcgattc tacccctgcg ccattgccat
    56941 agctgactga caaatgaccg cttaacgtcg gggtaaacga tgaccttatc ccagcctgat
    57001 aaacgccacg ggttccagcc acatcattat taaatttgcc atcgtcatta actttcacat
    57061 ggttagaatc ggcctattcc tgacgcacgg cggctttcag ccagggttcc aacgtcgtac
    57121 cgttttgcag gtccatgtga tagcttaccg ccgttcccgc ttcagcgcgt aatatccggg
    57181 tatttcccac atccgcgcgc atgccgtttg ataacgtgta gtcctgacca tctgtggtaa
    57241 agccggtaaa ggcgagatag gggctaacac tccacaatcc gtcaacccaa cggaacccgc
    57301 tctcaacatg agcacccgcg ccgttactat tgtaatcgcc aaacgctgtt gccccattac
    57361 tcatcttgcc atggatggtg ttggcaaaac ggtcaacttt caccacccca tcaacatagg
    57421 caccgttctg atgctcccaa ccggcataag cccccagggt gtagctatca atattgcctt
    57481 tgccgccgcg atcaaaacca atatcagaat gagagtaatc aaagaacaag ccgcgaattg
    57541 tgctgctttc ttcacgggag aaacggctat cgataccgag cgtcaggccc gtcaatgttt
    57601 gctcaaagcc agctcccgca tcagtggtca cgttgttgcg ggtgttaatt gccgaactcc
    57661 acatcgccgt atcgtaatta acgcctttta cgctaccaag acgctcacgc acggtgtcca
    57721 gttctgcatc aaataccagc ggttgtgctg ctgccatgtt cagcacatca gtggttgaag
    57781 gggtaatttg cgcgcgattc tctgcgaggc tccagctatg gttgccatta tccagcaagg
    57841 tatattcata cgtaccgata tcaacaacgc ctccggcatt gcccaacgtg aatgcagcat
    57901 caccgccgcc cgtcgttacc agtgtaaggc tatctcctgc tgccgggctg gcccccgtgt
    57961 ccgtcacgaa tattttgaaa tcacctgttg cctgaccgat gacgttgagc tgatcgctct
    58021 gatgattagc catatcggta cgcatataaa attgccgttt cccgacaggg tattggtggt
    58081 caacgtaatg aaattttccg cacttgccgt cacagatgaa cgagtcactg gctcagccat
    58141 cataatcaca tttgcgccgg acatcgcaag gttggctaag ttgaggcgag tgttttggtt
    58201 cgtcgtagtg atatcggcaa tgatggtcca tgcgctattt tcgagcgaaa cgtccgcttg
    58261 gctggtatcc acggcaccat gcgttctaaa agaagcgccc tcagaaacca ctaccttacc
    58321 caccaacata gcgggtgcat aatttatttt cggcttcatg acctcaagaa tgccatcatt
    58381 ccctctgact gacacgttaa ccattgtgcc agcccagaca ttagcgcggc cattgttgat
    58441 aaccatattt tcagagatgg ctttagagga atacgtgata cttccattct gatctgatcg
    58501 accgagatca tatacagcat ttgcctgcac tttcgtacca gtatcctttc ccagcgattg
    58561 catagtggca tgctcatcaa ggatagtgtc aatggcctga gtgtcctcca taacaatgag
    58621 cccggagcca tcatccagtt cataattttt tgacacacca tcctttatac taaattggcc
    58681 tttactgttt gtaccactca cttccagcgc attcgttgag acaattaatt tcccgcctgc
    58741 ttttttatca acgccagttg cagtcccgcc atccataacc tccagtaagc cgccactatc
    58801 cacagtggta ttataagcga agtcattttc ttcaactcgc aaacttccgc cgttttccaa
    58861 cagcatattg ttagcaatac cattcttgat ttcgaactga ccgagacggt ttgttccgaa
    58921 tacctccatg acccgcgtgg ttgttttaat agcaccgcct gctttctgat ctaccgcaaa
    58981 tgctaatccc ccgtctttaa cctccagtaa tcctcccttt tcgatagcgg caaagacggc
    59041 aatcccatct ttttcaacaa ttaagcgccc gcctttaatc gtgggatcat tcgccgc
//
LOCUS       DAESHI010000012        58389 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.012, whole genome shotgun sequence.
ACCESSION   DAESHI010000012 DAESHI010000000
VERSION     DAESHI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.012"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..161
                     /locus_tag="KW011_000764"
     CDS             <1..161
                     /locus_tag="KW011_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBH7659182.1"
                     /translation="WQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYR
                     AIYLRQES"
     gene            complement(199..1260)
                     /locus_tag="KW011_000765"
     CDS             complement(199..1260)
                     /locus_tag="KW011_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBH7659183.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1473..2936
                     /gene="bepA"
                     /locus_tag="KW011_000766"
     CDS             1473..2936
                     /gene="bepA"
                     /locus_tag="KW011_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HBH7659184.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLQERFKPYTKM"
     gene            2957..3316
                     /gene="arsC"
                     /locus_tag="KW011_000767"
     CDS             2957..3316
                     /gene="arsC"
                     /locus_tag="KW011_000767"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HBH7659185.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3454..4200)
                     /gene="hda"
                     /locus_tag="KW011_000768"
     CDS             complement(3454..4200)
                     /gene="hda"
                     /locus_tag="KW011_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HBH7659186.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="KW011_000769"
     CDS             complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="KW011_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBH7659187.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5625..6251)
                     /gene="upp"
                     /locus_tag="KW011_000770"
     CDS             complement(5625..6251)
                     /gene="upp"
                     /locus_tag="KW011_000770"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBH7659188.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6576..7613
                     /gene="purM"
                     /locus_tag="KW011_000771"
     CDS             6576..7613
                     /gene="purM"
                     /locus_tag="KW011_000771"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBH7659189.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7613..8251
                     /gene="purN"
                     /locus_tag="KW011_000772"
     CDS             7613..8251
                     /gene="purN"
                     /locus_tag="KW011_000772"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBH7659190.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8422..10488
                     /gene="ppk1"
                     /locus_tag="KW011_000773"
     CDS             8422..10488
                     /gene="ppk1"
                     /locus_tag="KW011_000773"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HBH7659191.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10493..12034
                     /gene="ppx"
                     /locus_tag="KW011_000774"
     CDS             10493..12034
                     /gene="ppx"
                     /locus_tag="KW011_000774"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HBH7659192.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12073..14316)
                     /gene="pdeF"
                     /locus_tag="KW011_000775"
     CDS             complement(12073..14316)
                     /gene="pdeF"
                     /locus_tag="KW011_000775"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HBH7659193.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(14498..14650)
                     /locus_tag="KW011_000776"
     CDS             complement(14498..14650)
                     /locus_tag="KW011_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659194.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKTSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            14668..14859
                     /locus_tag="KW011_000777"
     CDS             14668..14859
                     /locus_tag="KW011_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HBH7659195.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            15170..15688
                     /locus_tag="KW011_000778"
     CDS             15170..15688
                     /locus_tag="KW011_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBH7659196.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            15704..16243
                     /locus_tag="KW011_000779"
     CDS             15704..16243
                     /locus_tag="KW011_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HBH7659197.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16338..17915)
                     /gene="guaA"
                     /locus_tag="KW011_000780"
     CDS             complement(16338..17915)
                     /gene="guaA"
                     /locus_tag="KW011_000780"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBH7659198.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(17984..19450)
                     /gene="guaB"
                     /locus_tag="KW011_000781"
     CDS             complement(17984..19450)
                     /gene="guaB"
                     /locus_tag="KW011_000781"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBH7659199.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            19612..20982
                     /gene="xseA"
                     /locus_tag="KW011_000782"
     CDS             19612..20982
                     /gene="xseA"
                     /locus_tag="KW011_000782"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBH7659200.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(20979..21194)
                     /locus_tag="KW011_000783"
     CDS             complement(20979..21194)
                     /locus_tag="KW011_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBH7659201.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(21263..22735)
                     /gene="der"
                     /locus_tag="KW011_000784"
     CDS             complement(21263..22735)
                     /gene="der"
                     /locus_tag="KW011_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBH7659202.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(22853..24031)
                     /gene="bamB"
                     /locus_tag="KW011_000785"
     CDS             complement(22853..24031)
                     /gene="bamB"
                     /locus_tag="KW011_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HBH7659203.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(24042..24662)
                     /gene="yfgM"
                     /locus_tag="KW011_000786"
     CDS             complement(24042..24662)
                     /gene="yfgM"
                     /locus_tag="KW011_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HBH7659204.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(24680..25954)
                     /gene="hisS"
                     /locus_tag="KW011_000787"
     CDS             complement(24680..25954)
                     /gene="hisS"
                     /locus_tag="KW011_000787"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBH7659205.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(26065..27183)
                     /gene="ispG"
                     /locus_tag="KW011_000788"
                     /gene_synonym="gcpE"
     CDS             complement(26065..27183)
                     /gene="ispG"
                     /locus_tag="KW011_000788"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBH7659206.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(27210..28223)
                     /gene="rodZ"
                     /locus_tag="KW011_000789"
     CDS             complement(27210..28223)
                     /gene="rodZ"
                     /locus_tag="KW011_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HBH7659207.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(28508..29662)
                     /gene="trmG/rlmN"
                     /locus_tag="KW011_000790"
     CDS             complement(28508..29662)
                     /gene="trmG/rlmN"
                     /locus_tag="KW011_000790"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HBH7659208.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(29812..30243)
                     /gene="ndk"
                     /locus_tag="KW011_000791"
     CDS             complement(29812..30243)
                     /gene="ndk"
                     /locus_tag="KW011_000791"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBH7659209.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(30392..32704)
                     /gene="pbpC"
                     /locus_tag="KW011_000792"
     CDS             complement(30392..32704)
                     /gene="pbpC"
                     /locus_tag="KW011_000792"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HBH7659210.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(32705..37666)
                     /gene="yfhM"
                     /locus_tag="KW011_000793"
     CDS             complement(32705..37666)
                     /gene="yfhM"
                     /locus_tag="KW011_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HBH7659211.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            37873..38718
                     /gene="sseA"
                     /locus_tag="KW011_000794"
     CDS             37873..38718
                     /gene="sseA"
                     /locus_tag="KW011_000794"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HBH7659212.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(39211..39987)
                     /gene="sseB"
                     /locus_tag="KW011_000795"
     CDS             complement(39211..39987)
                     /gene="sseB"
                     /locus_tag="KW011_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HBH7659213.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(40129..41412)
                     /gene="pepB"
                     /locus_tag="KW011_000796"
     CDS             complement(40129..41412)
                     /gene="pepB"
                     /locus_tag="KW011_000796"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HBH7659214.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(41590..41790)
                     /gene="iscX"
                     /locus_tag="KW011_000797"
     CDS             complement(41590..41790)
                     /gene="iscX"
                     /locus_tag="KW011_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HBH7659215.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(41802..42137)
                     /gene="fdx"
                     /locus_tag="KW011_000798"
     CDS             complement(41802..42137)
                     /gene="fdx"
                     /locus_tag="KW011_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HBH7659216.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(42139..43989)
                     /gene="hscA"
                     /locus_tag="KW011_000799"
     CDS             complement(42139..43989)
                     /gene="hscA"
                     /locus_tag="KW011_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HBH7659217.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(44006..44521)
                     /gene="hscB"
                     /locus_tag="KW011_000800"
     CDS             complement(44006..44521)
                     /gene="hscB"
                     /locus_tag="KW011_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HBH7659218.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(44617..44940)
                     /gene="iscA"
                     /locus_tag="KW011_000801"
     CDS             complement(44617..44940)
                     /gene="iscA"
                     /locus_tag="KW011_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HBH7659219.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(44957..45343)
                     /gene="iscU"
                     /locus_tag="KW011_000802"
     CDS             complement(44957..45343)
                     /gene="iscU"
                     /locus_tag="KW011_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HBH7659220.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(45371..46585)
                     /gene="iscS"
                     /locus_tag="KW011_000803"
     CDS             complement(45371..46585)
                     /gene="iscS"
                     /locus_tag="KW011_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBH7659221.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(46826..47314)
                     /gene="iscR"
                     /locus_tag="KW011_000804"
     CDS             complement(46826..47314)
                     /gene="iscR"
                     /locus_tag="KW011_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HBH7659222.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(47615..48352)
                     /gene="trmJ"
                     /locus_tag="KW011_000805"
     CDS             complement(47615..48352)
                     /gene="trmJ"
                     /locus_tag="KW011_000805"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HBH7659223.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            48471..49274
                     /gene="suhB"
                     /locus_tag="KW011_000806"
     CDS             48471..49274
                     /gene="suhB"
                     /locus_tag="KW011_000806"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HBH7659224.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            49419..50273
                     /locus_tag="KW011_000807"
     CDS             49419..50273
                     /locus_tag="KW011_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH7659225.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            50464..51744
                     /gene="csiE"
                     /locus_tag="KW011_000808"
     CDS             50464..51744
                     /gene="csiE"
                     /locus_tag="KW011_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HBH7659226.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(51736..52875)
                     /locus_tag="KW011_000809"
     CDS             complement(51736..52875)
                     /locus_tag="KW011_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HBH7659227.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     SAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(53035..53925)
                     /gene="hcaR"
                     /locus_tag="KW011_000810"
     CDS             complement(53035..53925)
                     /gene="hcaR"
                     /locus_tag="KW011_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HBH7659228.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            54061..55422
                     /locus_tag="KW011_000811"
     CDS             54061..55422
                     /locus_tag="KW011_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HBH7659229.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            55419..55937
                     /locus_tag="KW011_000812"
     CDS             55419..55937
                     /locus_tag="KW011_000812"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HBH7659230.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            55937..56257
                     /locus_tag="KW011_000813"
     CDS             55937..56257
                     /locus_tag="KW011_000813"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HBH7659231.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            56254..57066
                     /gene="hcaB"
                     /locus_tag="KW011_000814"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             56254..57066
                     /gene="hcaB"
                     /locus_tag="KW011_000814"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HBH7659232.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            57076..58278
                     /locus_tag="KW011_000815"
     CDS             57076..58278
                     /locus_tag="KW011_000815"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HBH7659233.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
BASE COUNT        14270 a        15787 c        14570 g        13762 t
ORIGIN      
        1 tctggcaact ggcgcacagt agcgcagaca attttccggc actgacggta agccatttta
       61 ttaccgccaa tctgctcccg gtgatgctgg gtaatattat cggcggtgca gtgctggtga
      121 gtatgtgtta tcgggctatt tatttacgtc aggaatcctg aaaaatcagc ccggcgaaac
      181 agttcgtcgg gctgaagatt attcttgcgc tatttgccca tcgggccagg cgtggatcac
      241 ggctttgatc agcgtcgcca atggaatagc gaagaatacg ccccagaatc cccacaaacc
      301 accgaagatc accaccgata aaataatcac cagcggatgc aggttaaccg cttcggagaa
      361 caacaccggt actaacagat taccgtccag cgcctgaata atcagataca ctgcgaagca
      421 gctccagaat tccgtgcctg caccaaactg gaatagcgcc acgccaacca ccggaatggt
      481 caccacaaat gcgccaatgt acggaataag taccgagaag ccgaccagca ccgccagcag
      541 cagcgaatag ttcagcccaa agagcaagaa ccccagccaa gtggcgatcc ccaccacgat
      601 catctccagc actttgccgc ggatatagtt ggtgatttgt tgattcatct ccttccacac
      661 ctgtcctgcc agtccacggt tgcgcggcag cacccgacga acggcgttca gcatctgctc
      721 tttgtctttc agcaggaaga agaccatcaa tggtaccagc accagataga cggcaatggt
      781 cagcaaaccg accagcgagg cgagggaaat tttcaccacc gaatcgccca tggtcagcat
      841 ccgactgcgc atattttcgg ccattgcatc aataatgccc gcgtccatta atgccggata
      901 gcggcgcggc aacgtggcgg caaagtcaga aagcttattg agcatccccg gcatatcgcg
      961 gatcaagtag atgccctgtt gccaggcgat aggcagtacc acgaacgcca tcagtagcaa
     1021 tataccgacg aaaaccacca atacaatcga cgtcgcccag cggcgggagc agccaataga
     1081 ttgcaggcgc acggttggcc attccagcaa ataggccagc acaatagcca ccagcaacgg
     1141 agcaagcagg ccactaaaga aaaagataat gccaaatccg gcaactaaaa taaccagcaa
     1201 ggcaatcgct tccgggtcgc taaaacggcg gcgataccat tgcatcaaca tttcgagcat
     1261 aaagaccttc cctgaacctc aagagcggga ttgcgatccg caattgtatc gaaatgtcac
     1321 aaaaaagact tcgcttttta tgacggattc aggaaactga aaagtcattt gagtgggcta
     1381 atcttcgccg ttacactcaa aggcggcgcg gtgggaacaa tatttcacag tatcggtcaa
     1441 atgactatct tcagaaatac aggatagagg ttatgttcag gcagttgaaa aaaaacctgg
     1501 ttgcaaccct cattgctgct atgaccattg gtcaggtagc cccggcgttt gccgacagcg
     1561 cagacacctt gccggatatg ggaacctccg caggaagcac gctttccatt ggtcaggaaa
     1621 tgcagatggg cgactattat gtccgccagc tacgcggcag cgcgccgtta attaatgacc
     1681 cgctgttaac gcaatatatt aattcgctgg ggatgcgtct ggtttcgcat gccaattcgg
     1741 ttaagacacc gtttcatttt tttctgatca acaacgacga aattaacgcc tttgctttct
     1801 ttggcggcaa cgtggtgctg cactctgccc tgttccgtta ttccgataac gaaagtcaac
     1861 tggcttcagt tatggcgcac gaaatctccc acgtcaccca acgtcacctg gcgcgagcga
     1921 tggaagatca gcagcgcagc gcgccgctga cctgggtcgg cgcgttaggt tctattttac
     1981 tggcgatggc cagtccgcag gcggggatgg cggcgctgac cggtacactg gcgggaacgc
     2041 gtcaggggat gatcagtttc acccagcaaa atgaacagga agcggaccgc attggtattc
     2101 aggtgctgca acgctcggga ttcgatccgc aggcgatgcc aaccttcctc gaaaaattac
     2161 tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt attgactcac ccgttgccgg
     2221 aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat gcgcccgatg gtggtgcagt
     2281 cgtcggaaga tttctatctg gcgaaagcgc gcacactggg gatgtataat tccggacgta
     2341 accagctcac cagtgatttg ctggatgaat gggcgaaagg aaacgttcgt cagcaacgag
     2401 cggcgcaata tggtcgtgct ttacaggcga tggaagccaa taaatacgac gaggcgcgta
     2461 aaacgctgca accgttactg gcggcagaac ctggcaacgc atggtatctc gatctggcta
     2521 ccgatatcga tcttgggcaa aacaaagcca atgaggcaat caatcgcctg aaaaatgccc
     2581 gtgatttgcg caccaatccg gtgttgcagc tcaacctggc aaacgcttat ctgcaaggcg
     2641 gtcaaccaca agaagcggcc aatattctga atcgctacac ctttaataat aaagatgaca
     2701 gcaacggctg ggatttactg gcacaggcgg aagccgcgct aaataaccgc gatcaggagc
     2761 tggctgcgcg agcagaaggt tatgcgctcg ccggacgact cgatcaggcc atttcgctgt
     2821 tgagtagcgc cagttcgcag gtgaaattag gcagccagca acaagcgcgt tacgatgcgc
     2881 gcatcgacca gttgcgccag ctgcaggaac gctttaagcc ttataccaag atgtaattcg
     2941 cattaaagga agaacaatga ccaaacaggt aaaaatctac cataacccac gctgttcaaa
     3001 gagccgggaa acgctgaacc tgctgaaaaa aaacggcgtg gagccagaag tggtactcta
     3061 ccttgagaca cccgccgatg cggcaacgct gcgcgatttg ctgaaaatgc tggggatgaa
     3121 tagcgcccgt gaattgatgc gccagaaaga ggatctttat aaagaactga acctggctga
     3181 cagttcgctt agcgaagagg cgctgattca ggcaatggtg gataatccga agctgatgga
     3241 gcgcccgatt gtggtcgcga atggcaaagc gcggattggt cgtccaccgg agcaggtact
     3301 ggaaatcgtg ggttaagatg tgccccatcg ctagttgaag cacattgccg gatgcgacgc
     3361 ttgccatgtc ttatccggcc tacgaatact atggattaac ttcgcgtagt tcggataagg
     3421 cgttcgcgcc gcatccgaca ataaacacct tatctacaac ttcagaattt ctttcacaaa
     3481 cggaatggtc agcttacgtt gcgcggtaat cgacgcacga tccaactgat ccaacgtcat
     3541 aaatagcgtg cgcatttctc tgtcgagccg cttcagcaag aaacgcccca catcttccgg
     3601 cagttcaaaa ccacgcaaac gcgcgcgtaa ctgtagcgcc tgcaacttat cttcatcaga
     3661 aagtggctgc aatttgtaga tctgccccca gtcgagtcgc gacgcgagat ccggtaatcc
     3721 cagattcaac tgccgcggtg gacgatcgcc ggtgatcaac aatcgtgttt tgcccgattc
     3781 cagaattcga ttgtagagat cgaaaatcgc catctcccac aactcatcgc ctgcaataca
     3841 ctcaatgttg tcgatacaga ccagcgacaa atgctccata ccgtcgagca cttccggaac
     3901 aaaccaggtg cgtttatcca gcgggacata gcccaccgca tcgccacgct gcgacaattc
     3961 cgcgcaagcc gcgtgcagca gatggctgcg ccccgcgcct tcgcgtgccc agagatagat
     4021 gtaaccgcta tgttcctgac gcagcacgtt ttgcagcgca gccagtaaag aggagttatc
     4081 ccccggccag aaacttgcaa aggtttcgtc gtcaggaaga taaagtggca aagagagctg
     4141 tgccggtgtg ttcagagata cctcaaccag gatttcacaa aatcgcgaga agtttaccac
     4201 agaatcccat gatgtttgaa ccgggcagca acactgcccg gtcggtacat tatttgtctg
     4261 ttatatccgc gtcttctgcg tccagcacca cttcttcagg gcgcagcaca ctaatcagtt
     4321 tgaagatcaa acttaagccg ataccgacga tggtcgccag cgccatacct ttcagctccg
     4381 ccgcgccaat gtttaccttc gcgccactga caccgatgat caaaatcacg gaagtcagga
     4441 tcaggttctg tgctttgttg taatccactt ttgattcgat caaaacacga ataccggaag
     4501 caccgatgac accataaagc agcagcgaaa cgccgcccat caccggcaat gggatcatct
     4561 ggatagcggc agccagttta ccgacgcagg aaagcaggat agcgaaaatc gccgccccac
     4621 cgataaccca ggtactgtaa acacgggtga tcgccatcac gccaatgttt tctccgtaag
     4681 tggtatttgg cgtagagcca aagaagccgg agatcacagt cgacaggccg ttagcaaaca
     4741 tcgaacggtg cagacctgga tcgcgcagca gatctttttt gacgatatta gccgttacta
     4801 ccaggtgccc tacgtgttcg gcaataacca ctaacgccgc tggcagaata gtcagaatgg
     4861 caaaccactc gaagcgcggc gtatagaggg ttggcagcgc aaaccagtga gcattaataa
     4921 tcggcgtggt atcgacaatt cccattgcga aagagagcgc gtaccccacc agcacgccaa
     4981 ttaaaatcgg gataattgcc aggaaaccac ggaacagcac ggaacctaaa accgtgaccg
     5041 ccagggtggt aatagagatg atgatggttt tggagtctgg cgtttgccct tcagccggga
     5101 gtaaacccgc cataccggca gctacgcccg ccagctccag accgatgacg gcaacgattg
     5161 cgcccattgc cgcaggtgga aacagcacgt ccagccagcc ggtccccgct ttcttcacga
     5221 taaaagaaac caggcagaac agcacgccgc acataataaa gccgcccagc gcgacttcat
     5281 accctaacgg caacagtaac aataccggtg aaataaaggc aaagctggaa ccaagataag
     5341 ccggaatttt ccctttacag atgaagagat acagcagcgt tccaataccg ttaaataaca
     5401 gtacagtcgc cgggttaata tgaaataaga cgggcaccag gacggttgca ccaaacatgg
     5461 cgaacaaatg ttgcaaacta agcgggattg tctgtaaaag tggcggtctt tcactcaccc
     5521 cgatagcacg gcgcgtcata gtattatcct ctgtattatg tgttataggc gctttactca
     5581 aaaaaaagcc gactcttaaa gtcggcttta attattttta ttctttattt cgtaccaaag
     5641 attttgtcac cggcatcgcc gaggcccgga ataatgtatc cgtgctcgtt cagtccctgg
     5701 tcaatcgatg cggtatacag ttcgacgtcc gggtgcgctt tttccagcgc agcgatacct
     5761 tctggcgcag ctaccagcac cagaactttg atgctgctgc agcccgcttt tttcagcagg
     5821 tcgatggtcg cgataacgga accaccggtt gccagcattg ggtcaacgat cagcgccata
     5881 cgctcatcga tgttagaaac cagtttctgg aagtacggta ccggctccag cgtttcttca
     5941 ttacggtaca taccgacaac gctgatgcgc gcgctcggaa cattttccag cacaccgtcc
     6001 atcatcccca gaccggcacg cagaattggc acaacggtaa ttttcttacc tttgatctgg
     6061 tcgatttcta ccgggccgtt ccagccttcg atagttactt tttccgtttc gaggtcggcg
     6121 gtcgcttcgt aagtcagcag gctacccact tcggaagcga gttcgcgaaa gcgcttggtg
     6181 ctgatatctt gctcacgcat cagtcccagc ttgtgtttga cgagtgggtg tttgacttcc
     6241 acgatcttca tactctttct cctttgaggg gcagccacaa aaaaaatcga cggattatac
     6301 ctcctttctt caaggcggca atattctttt cgttgacttt agtcaaaatg ataacggttt
     6361 gagataaagt tattttatat tcaaatggtt atgaaagaag attattccat ccgaaaacta
     6421 acctttgccc tggcacaagt cttctttcgc cgcgcgtctg gggaaaagac gtgcaaaaag
     6481 gttgtgcaaa gcagtctcga aaacgtttgc tttccctgtt agaattgcgc cgaattttat
     6541 ttttctaccg caagtaacgc gtggggaccc aagcagtgac cgataaaacc tctcttagct
     6601 acaaagatgc cggtgttgat attgacgcgg gtaatgctct ggttggaaga atcaaaggcg
     6661 tagtgaagaa aacgcgtcgt ccggaagtga tgggcggtct gggcggcttc ggtgcgctgt
     6721 gtgcattgcc gcaaaaatat cgtgaacccg tgctggtttc cggcactgac ggcgtaggta
     6781 ccaagctgcg tctggcaatg gacttaaaac gtcacgacac cattggtatt gatctggtcg
     6841 ccatgtgcgt taatgacctg gtggtgcaag gtgcagaacc gctgtttttc ctcgactatt
     6901 acgcaaccgg aaaactggat gttgataccg cttcagcggt gatcagcggt atcgcggaag
     6961 gatgtctgca atcgggctgt tcactggtgg gtggcgaaac ggcagaaatg ccggggatgt
     7021 atcacggtga ggattacgat gtcgcgggtt tctgcgttgg cgtggtagaa aaatcagaaa
     7081 tcatcgacgg ctctaaagtc agcgacggcg atgtgctgat tgcactcggt tccagcggtc
     7141 cgcactcgaa cggctattcg ctggtgcgca aaattcttga agtcagcggt tgtgatccgc
     7201 aaaccaccga acttgatggt aagccattag ccgatcatct gctggcaccg acccgcattt
     7261 acgtgaagtc agtgctggag ttgattgaaa aggtcgatgt gcatgccatt gcgcacctga
     7321 ccggcggcgg cttctgggaa aacattccgc gcgtattgcc agataatact caggcagtga
     7381 ttgatgaatc ttcctggcag tggccggaag tgttcaactg gctgcaaacg gctggtaacg
     7441 ttgagcgcca tgaaatgtat cgcaccttca actgcggcgt cgggatgatt atcgccctgc
     7501 ctgctccgga agtggacaaa gccctcgccc tgctaaatgc caacggtgaa aacgcgtgga
     7561 aaatcggtat catcaaagcc tctgattccg aacaacgcgt ggttatcgaa taatgaatat
     7621 tgtggtgctt atttccggca acggaagtaa tttacaggca attattgacg cctgtaaaac
     7681 caacaaaatt aaaggcacca tacgggcagt tttcagcaat aaggccgacg cgttcggcct
     7741 tgaacgcgcc cgccaggcgg gtattgcaac gcatacgctc atcgccagcg cgtttgacag
     7801 tcgtgaagcc tatgaccggg agttgattca tgaaatcgac atgtacgcac ccgatgtggt
     7861 cgtgctggct ggttttatgc gcattctcag cccggcgttt gtctcccact atgccgggcg
     7921 tttgctgaac attcaccctt ctctgctgcc gaaatatccc ggattacaca cccatcgtca
     7981 ggcgctggaa aatggtgatg aagagcacgg tacatcggtg catttcgtca ccgatgaact
     8041 ggacggtggc ccggttattt tacaggcgaa agtcccggta tttgctggtg atacggaaga
     8101 tgacatcacc gcccgcgtgc aaacccagga acacgctatt tatccactgg tgattagctg
     8161 gtttgccgat ggtcgtctga aaatgcacga aaacgccgcg tggctggatg gtcaacgtct
     8221 gccgccgcag ggctacgctg ccgacgagta atgccccgta gttaaagcgc cagctctgcc
     8281 gctggcgttt ttcaattcac ctgtaaatcg caagctccag cagttttttt cccccttttc
     8341 tggcatagtt ggacatctgc caatattgct cgccataata tccaggcagt gtcccgtgaa
     8401 taaaacggag taaaagtggt aatgggtcag gaaaagctat acatcgaaaa agagctcagt
     8461 tggttatcgt tcaatgaacg cgtgcttcag gaagcggcgg acaaatctaa cccgctgatt
     8521 gaaaggatgc gtttcctggg gatctattcc aataaccttg atgagttcta taaagtccgc
     8581 ttcgctgaac tgaagcgacg catcattatt agcgaagaac aaggctccaa ctctcattcc
     8641 cgccatttac tgggcaaaat tcagtcccgg gtgctgaaag ccgatcagga attcgacggc
     8701 ctctacaacg agctgctgct ggagatggcg cgtaaccaga tcttcctgat taatgaacgc
     8761 cagctctccg tcaatcaaca aaactggctg cgtcattatt ttaagcagta tctgcgtcag
     8821 cacattacgc cgattttaat caatcctgac actgacttag tgcagttcct gaaagatgat
     8881 tacacctatc tggcggtgga aattatccgt ggcgatacca tccgttacgc gctgctggag
     8941 atcccatcag ataaagtgcc gcgctttgtg aatttaccgc cagaagcacc gcgtcgacgc
     9001 aagccgatga ttcttctgga taacattctg cgttactgcc ttgatgatat tttcaaaggc
     9061 ttctttgatt atgacgcgct gaatgcctat tcaatgaaga tgacccgcga tgccgaatac
     9121 gatttagtgc atgagatgga agccagcctg atggagttga tgtcttccag tctcaagcag
     9181 cgtttaactg ctgagccggt gcgttttgtt tatcagcgcg atatgcccaa tgcgctggtt
     9241 gaagtgttac gcgaaaaact gactatttcc cgctacgact ccatcgtccc cggcggtcgt
     9301 tatcataatt ttaaagactt tattaatttc cccaatgtcg gcaaagccaa tctggtgaac
     9361 aaaccactgc cgcgtttacg ccatatttgg tttgataaag cccagttccg caatggtttt
     9421 gatgccattc gcgaacgcga tgtgttgctc tattatcctt atcacacctt tgagcatgtg
     9481 ctggaactgc tgcgtcaggc ttcgttcgac ccgagcgtac tggcgattaa aattaacatt
     9541 taccgtgtgg caaaagattc acgtatcatc gactcgatga tccacgctgc gcacaacggc
     9601 aagaaagtga ccgtggtggt tgagttacag gcgcgtttcg acgaagaagc caacattcac
     9661 tgggcgaagc gcctgaccga agcaggcgtg cacgttatct tctctgcgcc ggggctgaaa
     9721 attcacgcca aactgttcct gatttcacgt aaagaaaacg gtgaagtggt ccgttacgca
     9781 cacatcggga ccgggaactt taacgaaaaa accgcgcgtc tttatactga ctattcgttg
     9841 ctgaccgcag atgcgcgtat caccaacgaa gtacggcggg tatttaactt tattgaaaac
     9901 ccataccgcc cggtgacatt tgattattta atggtgtcac cgcaaaactc tcgccgtctg
     9961 ttatatgaaa tggtagaccg cgaaatcgcc aacgcgcagc aagggctgcc cagtggtatc
    10021 accctgaagc taaataacct tgtcgataaa ggcctggttg atcgtctgta tgcggcctcc
    10081 agctccggcg taccggttaa tctgctggtt cgcggaatgt gttcgctgat ccccaatctg
    10141 gaaggcatta gcgacaacat tcgtgccatc agtattgttg accgttacct tgaacatgac
    10201 cgggtttata tttttgaaaa tggcggcgat aaaaaggtct acctttcttc cgccgactgg
    10261 atgacgcgca atattgatta tcgtattgaa gtggcgacac cgctgctcga tccgcgcctg
    10321 aagcagcggg tgctggacat catcgacata ttgttcagcg atacggtcaa agctcgttat
    10381 atcgataaag aactcagtaa tcgctacgtt ccccgcggca atcgccgcaa agtacgggcg
    10441 cagttggcga tttatgacta catcaaatca ctcgaacaac ctgaataacc ctatgccaat
    10501 acacgataaa tcccctcgtc cgcaggagtt tgctgcggtc gatcttggtt caaacagttt
    10561 tcacatggtc atagcccgtg tggtagatgg tgccatgcag attattggcc gcctgaaaca
    10621 gcgggtgcat ctggcggacg gcctggggcc agataatatg ttgagtgaag aggcaatgac
    10681 gcgcggtttg aactgtctgt cgctgtttgc cgaacggcta caagggtttt ctcctgccag
    10741 cgtctgtata gtcggcaccc atacgctgcg tcaggcgctg aacgccactg actttctgaa
    10801 acgcgcggaa aaggtcattc cctacccgat tgaaattatt tccggtaatg aagaagcccg
    10861 tctgattttt atgggcgtgg aacataccca accggagaaa ggtcgcaaac tggttattga
    10921 tattggcggc ggatctacgg aactggtgat tggtgaaaat ttcgaaccga ttctcgttga
    10981 aagccgccgg atgggttgtg tcagctttgc ccagctttac ttccctggcg gggtcatcaa
    11041 taaagagaat tttcagcgcg ctcgtatggc ggcagcacaa aaactggaaa ctttaacctg
    11101 gcaattccgt attcagggct ggaacgtggc aatgggcgct tccggtacca taaaagccgc
    11161 ccatgaagtg ttaatggaaa tgggcgagaa agacgggata attaccccgg aacgtctgga
    11221 aaaactggta aaagaagttt tacgtcaccg taatttcgca tcgctgagtt taccgggtct
    11281 ttccgaagag cggaaaacag tcttcgttcc tggactggcg attttatgcg gtgtgtttga
    11341 tgctttagcc atccgtgaac tgcgcctttc tgacggggcg ttacgcgaag gcgtactgta
    11401 cgaaatggaa ggacgtttcc gtcatcagga tgtgcgtagt cgcaccgcca gcagcctcgc
    11461 caaccagtat cacatcgaca gcgaacaggc ccgacgggtg ctggatacca ctatgcaaat
    11521 gtacgaacag tggcgggaac agcaaccgaa gctggcgcat ccgcaactgg aggcgctact
    11581 gcgatgggcc gccatgctgc atgaggtcgg gttgaatatc aaccacagcg gtttgcatcg
    11641 ccactccgct tatattctgc aaaacagtga cttgccgggt tttaatcagg aacagcagct
    11701 gatgatggcg acactggtgc gctatcaccg taaagcgatt aaactcgacg atctgccgcg
    11761 ctttaccttg tttaagaaga aacagttcct gccactgatt cagctattgc gccttggcgt
    11821 attactcaac aatcaacgtc aggcaaccac cacaccgcca acattgacac tgattactga
    11881 tgacagtcac tggacactgc gtttcccgca tgactggttt agtcagaatg cgttggtact
    11941 gcttgatctg gaaaaggagc aagaatactg ggaaggcgtg gctggctggc ggttgaaaat
    12001 tgaagaagaa agtacacctg aaatcgccgc ttaatgcttt gcgggccgac gagaaatgtc
    12061 ggcccgcatt attcaggcac tttcgcgaat gggttcgatt tcattcaacg tatcaattaa
    12121 cggctgcggc ttaccaataa gataaccctg catataatcg atccccaaag agagcaccgc
    12181 ctcgcggatc tcttcgtttt caacgtactc tgccactacc cgcattttct tcattcgcgc
    12241 cagatggcaa atcgacgcca caatctgata atccagacta tttgacacaa tattgcggat
    12301 aaagctgccg tcaattttaa gcagatcggc attcacattt ttaagccgtg catagctggc
    12361 gtagccggtg ccgaaatcat caatcgcaat ctggcagcct aattcctgaa gatgctgcaa
    12421 ggtgatttgc gcctgcttaa cattggtcag agcattactt tcggtgactt caaaaataag
    12481 ttgccacgct tcaatctgat atttagccag caactgactg acttcaacag gaaaacgagc
    12541 ctgacatacc gaggttggag acagattaat agcaaaacgg tgagcgggca tcttcgctct
    12601 gttttcagcc ataaattgca gcgtatgctc aatgacccac atgtcgatac tcgacgataa
    12661 accaaattcg tgcgcgaccg gcaagaagct atcggggacg atcagttcat cattctcacc
    12721 tttcatgcgc agaagaattt catggtaaac atcaccacgc ataccggtaa tcggctgggc
    12781 catcaggaaa aaatggttgt gttccagcgc ctgctgtagt cgattcatca tcgcgacttt
    12841 atctttcaat tcgcgttgca aatacattgc cccgcgacgc tgcatatttt ccggggcgtt
    12901 ggtcacgatg gaaagttcgg cgaccgtatt tagctctccc agcagcaggt agatatgatt
    12961 cactggcgag cgcacatagc agtaactgac gccaatctgc ggttgcatcg gcatcccatc
    13021 ccaaaagaaa cgaaattgct tgagatgact atccagtgcg gtaatgcgct cctggtgcga
    13081 ttctgtattc agtcgcagcg cgagatcgtt acccgaaagc tgataaacat cttcacccgg
    13141 ttccagcaag ggtgacagcc agtgagaaag tttttgcttg tattgaatcc gcagcatgat
    13201 gccatagttc ttaaccagca tttccatgcc agggatgcgt aaataacaaa gcgcagacca
    13261 gggagcatca cgcaacgcgc gattcagggc gcgaacattt ggcagatgaa ccaccgggtc
    13321 catatacgca agccgctgaa tgcgtctgac taccgctctc tgacgggttg ccagtacagc
    13381 catataattg acaataaaag agaataccag ataactggag gaggttatgg tcagctgcgt
    13441 ggtatagcca ggataaatgg gaatgtaatt ttgatagctg tggatgctga tcatcaacac
    13501 gaccgcccag agcagcgaaa tcagcttata accatagcgc atcgctcccc acatcatcag
    13561 gggcagcaat aatgacaagg tataattggt gctaaaaatt gtgctttttt catttaacgg
    13621 catgcataac agcaacaata aagcacctaa tgccagtagc cagagcgcga actctttttt
    13681 ggtgactttg gcatcaacct gctgttttaa ttgcgaataa tagctacgta aataaaatgg
    13741 atttcgcact acccgaatga tgaagtagca cagcgggaca ccgatcagat tacccaccag
    13801 taaggcctga taattgatta aagtcccgag gttaaaaggc atgacaccga ccagattttc
    13861 tctgctcgcc agtaatccta caaatgcagc aaactggaaa agtatcagaa acagcgttgc
    13921 aggaaacaca atctgccaga atatacgctg ggaaattaaa cgggtatcac catgtgaaac
    13981 gttgttacgc cggggagtaa agaccctgta accgccccaa cagagaatga taatgataat
    14041 aaaattagcc gttaatgata gcgtttcgta aaaacccagc tctgcatatt tgcggacaaa
    14101 aatccctaag gcgatccctg gcaatgccgc ccagctgaaa aacatcatca tgctgatcat
    14161 cagtgccaga ggcagataaa agagaaaaac ctcaccggaa gaaatatgcg cgaaagtatt
    14221 aatgtgggcg aaaatgggca agattaaaga aggcaggaac agcggaagcc cccaccattt
    14281 atcacgcatt tttatataag ttgcattcag tttcatagat gctcagcaga atcccccaca
    14341 tcctgaagga ggtgtattca gacaggcatc ccacctgact tcgaatgatg attattcatc
    14401 actatagaga gcattgattc taagtgtcat atgaaagttc caattgatat atatcaaaca
    14461 aaataaccct gattaatgaa ttattacgtt tatcatgtta attcatcatt attacatcat
    14521 cattgtaaat aattaaatta acttccataa cattaaaata tgtatccact gacgtttttt
    14581 tacataacga agaattgacc attttgtcct gttgtgcctt aatgtaagta ccgtccacag
    14641 cgtgggacat acttcaagga accttttgtg agtcaggcaa ccagtatgcg aaaacgacac
    14701 cgatttaaca gtcgcatgac ccgtatcgta ctgctcatca gctttatctt cttctttggc
    14761 cgttttatct actcgtccgt cggtgcctgg cagcaccatc agagcaaaaa agaagctcag
    14821 caatccacac tctccgtcga atcaccggta caacgttagc ggttaccttc ttcactttca
    14881 cagaacataa cggcacttcg ctgtcggatg cttttgctct ttgggattat caaagcggca
    14941 gatattcttt catcttaaat tttacgtctt tgtcctgact gatgtttatc ctgtttggct
    15001 gcggaataaa tataaaatta atatatatgt tgtaatgata tatttttata aattattccc
    15061 tgcgtgaatt ttaataaatt taatctatcc ctttatacgc aatacattta ctttcctctt
    15121 ttgatgatct taaatgtctt atttttcgta atgtgtataa caaggaatag tgatgaaatt
    15181 taaaaaatgt cttctgcctg tggcaatgtt agcgtcattc actctggcag gatgccagtc
    15241 aaatgctgac gatcatgccg ccgatgttta tcaaaccgat caactgaata ccaaacaaga
    15301 aactaaaacc gttaatatta tttccattct tcccgcaaaa gttgccgtag acaactccca
    15361 aaataaacgg aacgcacaag ccttcggcgc gcttattggc gcagtcgctg gcggtgttat
    15421 cggccacaac gtcggttctg gcagcaattc cggaacgacg gcaggggcag ttggcggcgg
    15481 agctgtaggc gcggcagcgg gttctatggt gaatgataaa accttagtgg aaggtgtttc
    15541 tttaacatat aaggaaggca ccaaagtgta tacctccacc caggtgggta aagagtgcca
    15601 gtttacgaca ggtttagccg ttgttattac cacaacgtat aacgaaacgc gtattcagcc
    15661 aaatactaaa tgtcctgaaa agagctaata atcaggagga gtcatgaaga aagtttttct
    15721 ttgcgccatc ttagcctcct taagctatcc ggctatcgcc tcatcattgc aggatcaact
    15781 ctctgctgtc gcagaagcgg aacagcaagg taaaaatgaa gagcaaaggc agcatgacga
    15841 atgggtcgcg gagcgcaaca gggaaatcca gcaagagaag caacgtcgcg caaatgccca
    15901 ggccgccgct aacaaaagag cggcaacggc agcggcaaat aagaaagctc gtcaggataa
    15961 actggacgcc gaagccactg cggacaaaaa acgcgatcaa agttatgaag atgagctacg
    16021 cagcttagag attcagaaac aaaaactgac gctggcgaaa gaagaagccc gcgttaagcg
    16081 agaaaacgaa tttatcgatc aggaactgaa gcacaaagct gcgcaaaccg atgtggtgca
    16141 atctgaagct gacgccaaca gaaatatgac tgaaggcggt cgcgatctga tgaaaagcgt
    16201 gggcaaagca gaagagaaca aatcggacag ctggtttaat taatcgatgt tagtaacttc
    16261 aagcctatga ttcttgaaga taaaaaaccc tctgtagtaa cagagggttt tgttcattca
    16321 tagtgcaggg attaaaatca ttcccactca atggtagctg gcggcttgcc gctgatgtca
    16381 tacaccacgc gggaaatacc gttcacttca ttgataatgc ggttggaaac gcgaccgagg
    16441 aaatcgtacg gcagacgcgc ccagtgtgcg gtcataaagt cgatggtttc gacagcacgc
    16501 agagagacaa cccagtcata cttacgacca tcgcccatta cgccaacgga acgtaccggc
    16561 aggaacacag tgaacgcctg gctgactttg tcgtacaggt ccgctttacg cagttcttca
    16621 atgaagatgg cgtcagcacg gcgcagcagg tcacagtact ctttcttcac ttcacccagc
    16681 acacgaacgc caaggcctgg tcccgggaat gggtgacggt acaacatgtc gtacggcagg
    16741 cccagctcca gaccaatctt acgcacttcg tctttgaaca gctctttcag cggttcaacc
    16801 aggcccatct tcatctcttt cggcaggccg cccacgttgt ggtgagattt gatgacgtgt
    16861 gctttaccgg ttgcagacgc cgcagattcg ataacgtcag ggtagatggt gccctgcgcc
    16921 agccacttca cgtcttccag tttcagcgct tcttcatcga atacttcaac gaaaacgcga
    16981 ccgatgattt tacgttttgc ttccggatcg ttttcgccag ccagcgctga caggaagcga
    17041 tcttctgccg gaacgtgaac aatgttcaga ccaaagtgat cgccaaacat atccagaacc
    17101 tgctctgctt cgttgagacg cagcaggccg ttgtcgacga atacgcaagt caggttttta
    17161 ccgatagcgc ggtgcagtag cattgcggtt acggaggaat ccacgccacc agagaggccg
    17221 aggatgactt tatcgtcgcc tacctgctca cggatgcggg ctacagcatc gtcgataatt
    17281 ttcgctggcg tccacagggc ttcacactgg cagatatcac gcacaaaacg ctccagcatg
    17341 cgcataccct ggcgggtgtg agtcacttcc gggtggaact gtacgccata gaagcgtttt
    17401 tcttcgttag ccataatggc aaacgggcag ctttcggtgc tggctacggt gacgaagtcg
    17461 gacgggatag cggtaacttt atcgccgtgg ctcatccaga catcgagcag cggtttaccg
    17521 tctgcggtca gcgcatcttc gataccgcga accagtgcac tgtcgtttac gacttcaacc
    17581 tgcgcgtagc caaattcacg ttcgttagag gcttcaacgt gaccgcccaa ctgcattgcc
    17641 atggtctgca tgccatagca aacgccgaat accggtacgc ctgcttcaaa gacatactgc
    17701 ggcgcacgcg ggctgttttc ttcagtagta ctttccgggc cgccggaaag aataatgccg
    17761 cttggattga agtcacgaat ttgtgcttct gtcacatccc acgcccacag ttcgcagtaa
    17821 acacccagct cacgcacgcg gcgcgcaacc agttgagtgt actgagaacc gaagtccaga
    17881 atgaggatgc gatgcttatg aatgttttcc gtcattgacg cttattccga ggcaagtgaa
    17941 acagataata taaatcgccc gacatgaagt cgggcgaaga gaatcaggag cccagacggt
    18001 agttcgggga ctctttagta atggtcacgt cgtgaacgtg gctttcctga atgcccgcac
    18061 cgctgatacg tacaaactcc gctttagtac gcagttcgtc gatagtacca cagccggtca
    18121 gacccataca ggagcgcagg ccgcccatct gctggtgaat gatctctttc aggcgacctt
    18181 tataagctac gcgaccttcg ataccttccg gcaccagttt gtcggcagcg ttatcgctct
    18241 ggaaataacg gtcagaggaa cctttggaca tcgcgcccag ggaacccata ccacggtaag
    18301 atttgtaaga acggccctgg tagagttcga tttcacccgg agattcttca gtacccgcca
    18361 gcatggaacc taccatcacc gcgcttgcgc cagcggcgat agctttggcg atgtcgccgg
    18421 agaagcgaat accaccatct gcgataaccg gaataccggt gccttccagg gcttcaactg
    18481 cgtcagcaac ggcagtgatc tgcggaacac ctacgccagt aacgatacga gtcgtacaga
    18541 tagaaccagg gccgataccg actttaaccg cgctgcaacc agcttctgcc aaagcgcgtg
    18601 cacctgcagc tgttgccacg ttgccgccga tgatttgcag atccggatat ttagcacggg
    18661 tttcacggat acgctgcaaa acgccttctg agtgaccgtg ggaggagtcg atcagcagaa
    18721 cgtcaacgcc agcggcaacc agcgcgtcaa cacgctcttc gttacccgca cctgcgccaa
    18781 ctgcagcacc aacacgcaga cggccttgct cgtctttaca ggcgttcggt ttacgttccg
    18841 ctttctggaa gtctttcacg gtgatcatgc cgatcaggtg gaattcgtca tcaaccacca
    18901 gcgctttttc aacgcgtttt tcgtgcattt ttgccagcac cacttcacgg gcttcacctt
    18961 cacgcacggt gaccagacgc tctttcggcg tcatgtaaac gctaaccggc tggttcaggt
    19021 cggtaacaaa acgcacgtca cgaccggtga taatacccac cagttcgttt tcttcggtaa
    19081 cgaccggata gcccgcaaaa ccgttacgct cggtcagttc tttcacttcg cgcagcgtcg
    19141 tggttggcag aacagtctgc ggatcagtca ccacaccaga ttcgtgtttt ttcacacggc
    19201 gaacttcttc tgcctggcgt tcaatggaca tgtttttgtg gataaagccg ataccgcctt
    19261 cctgagccag agcaatagcc aggcgcgctt ccgttacggt atccattgct gcggaaagca
    19321 tagggatatt cagacgaata gttttcgtca gctgggtgct gaggtcagca gtattcggca
    19381 gaacggtaga atgagcagga acgaggagaa cgtcgtcaaa cgtcagagct tctttagcga
    19441 tacgtagcat gggcaatatc tcgaccagag tggttaataa atattgccgc ggcattatac
    19501 agagcgtaac cgattgcatc tacccctttt tgcaaaaaat gcttgctatc cccgaagggc
    19561 gggttactat cgactgaata acctgctgat ttagaatttg atctcgctca catgttacct
    19621 tctcaatccc ctgcaatttt taccgttagt cgcctgaatc aaacggttcg tctgctgctt
    19681 gagcatgaga tgggacaggt ttggatcagc ggcgaaatct ctaatttcac acaaccggct
    19741 tccggtcact ggtactttac actcaaagac gacaccgccc aggtacgctg cgcgatgttc
    19801 cgcaacagca accgccgggt gaccttccgt ccacagcatg gacaacaagt tttagttcgc
    19861 gccaatatta cgctctacga gccgcgcggt gactaccaga taatcgttga gagtatgcag
    19921 ccggccggtg aagggctgct gcaacagaag tacgaacagc tcaaagcgaa gttgcaggct
    19981 gaaggtttgt tcgatcagca atacaaaaaa ccacttccct cccctgcgca ttgcgttggt
    20041 gtgatcacct caaaaaccgg tgctgcgcta catgatattt tgcatgtgtt aaaacgtcgc
    20101 gatccttctc taccggtgat catctacccc accgccgttc agggcgatga cgcaccgggg
    20161 caaattgttc gcgccattga actggcgaat cagcgcaatg agtgcgacgt gttgatcgtt
    20221 gggcgcggcg gcggttcgct ggaagattta tggagtttta acgacgaacg cgtagcgcgg
    20281 gcgatttttg ccagccgcat tccggtcgtc agcgccgtcg ggcatgagac ggatgtgacc
    20341 attgccgatt ttgttgccga tctgcgtgcg ccaacgccgt ctgccgccgc tgaagtagtg
    20401 agccgtaatc agcaagagtt actgcgccag gtgcaatcga cccgtcaacg gctggagatg
    20461 gcgatggatt attatctcgc caaccgcacg cgtcgcttta cgcagatcca tcaccgatta
    20521 cagcaacagc atccgcagct ccggctggca cgccagcaaa ccatgcttga gcgcctgcaa
    20581 aagcggatga gctttgcgct ggaaaatcaa cttaagcgta ccgggcaaca gcagcagcgg
    20641 ttaacacagc ggctgaatca gcaaaatcca cagccgaaga ttcatcgcgc gcaaacgcgc
    20701 attcagcaac tggaatatcg tttagcagaa accctgcgcg tacagcttag cgccacgcgt
    20761 gaacgtttcg gtaatgcagt aacgcacctc gaagccgtaa gcccactgtc aacgctggcg
    20821 cgtggataca gcgttactac tgctactgac ggcaatgtac tgaaaaaagt gaagcaagtt
    20881 aaagcgggtg aaatgctaac cacacgtctg gaagacggct ggatagaaag tgaagttaaa
    20941 aacatccagc cagtaaaaaa atcgcgtaaa aaggtgcatt aagccagcac gaattcaacg
    21001 cgttttttcg atatcaagcc atgcccgtgc tgacagaagt aatctactgc accgcaggct
    21061 tttaacacct gcaacggttg atggcagtct ggacaaagag ctttcatttc aataatttcc
    21121 ccgcaggatg ggcaacgcgc atggccgtta tcctgatcaa gcacatgctg acattgtgga
    21181 caatgcagtt ccatatgaat tcctccggta gccatccctc tacattcata gagggaatgg
    21241 cagataaaat acttacggat aattatttat ttttcttgat gtgcttcatc agacgcttac
    21301 gtttacgcat ctgggttggc gtcagggtgt tacgcttgtt cgcatacggg ttttcccctt
    21361 ctttgaactg aatacgaatc ggcgatccca ttacgtccag cgatttgcgg aagtagttca
    21421 tcaagtagcg cttgtaggaa tcaggcaggt ctttcacctg attaccgtga atcaccacaa
    21481 tcggcgggtt ataaccacca gcgtgggcat atttcagctt cacacgacga ccgcgtacca
    21541 gcggcggttg gtgatcttca acagccatcg tcatgatgcg cgtcagcata gaggtcccca
    21601 cacgacgggt ggagctgtca tacgcttcac gtactgattc aaacaagtta ccaacaccac
    21661 tgccgtgcaa ggcagagata aagtgcacac gagcaaaatc gataaagccc agacggaagt
    21721 ccagcgtttc tttcacctgc tctttcactt cctggctcag gccatcccac ttattcacca
    21781 caatgacaag tgagcgccca ctattgagaa taaagcccag cagcgagaga tcctgatcgg
    21841 aaataccttc gcgcgcatca atcactaaca tcaccacgtt ggtatcttca atggcctgca
    21901 acgttttgat tacggagaat ttctctacag catcggtgat tttgccgcgt ttacgtacgc
    21961 cagcggtgtc aatgagcaca tactcacgtc catcgcgttc cattggaatg tagatactgt
    22021 cacgcgtcgt gccaggcatg tcataaacaa caacgcgctc ttcaccaaga atacggttag
    22081 tgagtgtaga cttacctacg ttcggacgac ccacaatcgc cagtttgatc ggcagacttt
    22141 gcgggtcgaa gtcgtcttcc tcttcttctt cgccgttctc ttccgcttca aattgcgccc
    22201 agtattcagc gtcttcgtcg acttcctctt gcggtgcgag atcttccatc cacggcagca
    22261 gcacacgctc cagcagactt aatacgccac gaccgtgaga tgcggcgatc gggtagattt
    22321 cacctaaacc aagcgagtag aaatcaacca ctgcctgatc gggatccaga ccgtcagttt
    22381 tgtttgccac caggaaggtc ggtttttcac gagagcgcag atgtttggca atcgcttcat
    22441 ctgccggcat caagcccgcg cgcgcatcca ccataaacag tacgacgtcc gcttcttcaa
    22501 tcgccagcag cgactgttcc gccatgcggg tttctacacc gtcttctgtg ccatcaatcc
    22561 cgccggtatc aatacagata aactcacggc cttcaatttc cgcacgaccg tacttacggt
    22621 cacgagtcag acccgggaaa tccgcaacca gcgcatctcg ggtgcgagtt agacggttaa
    22681 ataacgtgga ttttcctacg ttagggcgcc cgacaagcgc gaccacaggt accatgttta
    22741 aagcctcatt tttataaatc atcagacaac gcacgctata ttcgcgtcgt tgttaaaaac
    22801 aggaaaacgg cccctgtcca ggagccgttt tcaaagtgaa cgacagagac gattaacgtg
    22861 taatagagta cacggttccg tcttttgcct ggatcagcag tttgccgtca gcggcaaccg
    22921 gttcagtctg gaaaccggaa ctatcaactt tttgctgggc aacgaaacga ccatcttcga
    22981 cgttaatcca gtgcagataa ccttcactgt cacctaccac caggttgcca ttatacagca
    23041 ccggcgaagt cagcaggcga tgcagcagat cgctttgtgt ccacagcgta acgccgccat
    23101 caatggtcaa cgccatcacc cggtcatttt gatcgaccag atagatgcga ttgccgtcga
    23161 cgatgaaatc attcaccgaa cccagttcgc gtttccacat aatctgacca ctgcgcagat
    23221 caagcgccgt caggttacca ttataggcca gagcgaaaac aacgccgtta acaacgacgg
    23281 gagtcgtgtc aacatcgctc agacggtcaa tttcggtaga accggtcgcc tgggaaatac
    23341 gctgctgcca aatcatctgg ccctgttcca tcagcactgc gctgacgcga ccattatcgc
    23401 cccccacgac ggccgcacca aaagccgttg ccggcgcaga ctcgccacgc aaagagagcg
    23461 aaggcatatc gaggttaact gtccatttga cagcgccgtc agcttcgttc agcgcttgta
    23521 actgaccgtt actagtgtgg attaacacca gaccgtcgct gaccaccggg cgagaaagtg
    23581 cttcacccgc gactttagtt tgccatgcca cagtaccatc gctggtattc agcgcgtaaa
    23641 cctgcgcctt ttcgctgcca atgtagacat gcccaccaga cacggtcaca ccgccagaaa
    23701 gtaatgcagg ctctttagag aaccagccat ctttctcggc caggctgaca gaccagattt
    23761 ctttgccatc atccgcattc agcgctttta ccaaaccagc gcggtccgct gcatagacaa
    23821 cgttgtccgc cagtgccgga tgaagattgg aatagaagtt gccaatgccg ctaccaacgg
    23881 aagtgctcca cgccgtggtc ggcgtaaact ggttttcaac ggttggcaat ggggacatct
    23941 ttaccacatc ttcttcgctg ttaaacagcg aacagccgct taaaagggta acggaaagca
    24001 gtcctggcag cagtaattta cgcaattgca tcgggtccct ctcagatgga caaattatta
    24061 attttcatct gcatcatttc gctcagtgcc ggagtaacat cgcttttcac gcctgcttcc
    24121 catgcactac gcgcaccttg cttatcacct ttgctcaaca atgctttacc acgcaggtcg
    24181 gcaacaatgg cagcccaccc ttcacctttg atggtatcaa gggttttcag cgcggcatca
    24241 gcctgcttga gctgtacctg aacgcgagca agacgcagat ttatcacggc tttgagattt
    24301 tcatcactcg tgtctgccag cccctgttgt aactgggcgg cagctttctc tagttcattt
    24361 ttgtcaacaa attgctgcgc aagttccaaa gaagccagcg caccataagt atttttattt
    24421 tcagcagcaa atttttccgc cgccgggatg ctatccggtt tgccttcgct cactgcggta
    24481 accgcatttt gataggcaag agaagcggag cgtgcagaat caacctgatg gctgttccag
    24541 tagcgccagc caatcagtgc gccaacgccc aaaatcaccc caacagccag tgctttgcca
    24601 ttttcagcaa aaaagcgttt aaccgcttct acctggtcgt tttcgttctc gtaaatttcc
    24661 acgctgtcct tctccttcct taacccagta acgtgcgcaa atgcgcggct acgctatcct
    24721 gcgcaactgc cgtttgctca ccagagcgca aatccttcac tactgctgtg ccgttagcca
    24781 cttcagactc acccagcacc acagcaacgc gggcacccca tttatcagca cgggcaaact
    24841 gtttcttaaa gttgccgccg ccgtggttgg tcatcaattt cacgcccggt aattcatcac
    24901 gcagacgctc agctaatgcc atagccgcag attgtgtatc agcacctgaa gccaccaggt
    24961 atatatcgac aacaggatcg gctttaaatt ccggattaac ggcctgtact aacaatacaa
    25021 gacgttcgag gcccatcgca aaaccgactg ccggtgttgc acgaccgccc agttgttcca
    25081 ccagaccgtc ataacgaccg cctgcacaca cggtgccctg ggagccgagg ctgttagtca
    25141 cccactcgaa aacggtacgg ttatagtaat ccagaccacg caccagacgc tggtttacgg
    25201 tgtaagcgat ccccgcgctc tcaagcagtt tgcacagacc ggcaaaatgc tcacgagatt
    25261 cctcgtccag ataatcacct aatgccggag cgtcgttgag aagcgcctgc acttccggat
    25321 tttttgaatc cagcacgcgc agcgggttag tgtacatgcg gcgtttgcag tcttcgtcca
    25381 gcttttcttt atgctgctca aggaatgcca ccagcgcatc gcggtaattg gcgcgtgctt
    25441 ccagcgaacc aatagagttc agctcaagag ttacgtgttc gaagataccc agcgcacgcc
    25501 accagcgggc agtgagcata atcagttcag cgtcgatatc cggaccttgc agaccgaaaa
    25561 cttcgcagcc caactgatgg aactgacgat aacgcccttt ctgcggacgc tcgtgacgga
    25621 acatcggccc gatataccac agacgctgtt cctgattgta cagaagacca tgctcgatgc
    25681 cggcgcgtac acagcccgcc gtcccttcag ggcgcagagt caggctgtcg ccattgcgat
    25741 cctcaaaggt gtacatctct ttttcaacca cgtcggtgac ttcaccaatc gcacgtttga
    25801 atagcggggt ctgctctaca atcggcaagc ggatttcact gtaaccgtag ctgccgagca
    25861 cgtttttcag tgtgccttca atgcgctgcc agatggccgt ttcgccaggc aggtaatcgt
    25921 tcatgccgcg aatggcttga atgttttttg ccacgtttat tctctttctg aatataaaaa
    25981 tgaaccctca acgcttccct caatgtttcg ggagccatgc gggttcaatc atacacggga
    26041 agcgaggcgc ttcccatcac gttattattt ttcaacctgc tgaacgtcaa ttcgacgcgc
    26101 ttcgtccagc tgactggctt tcgcacgaat gcgtgcttcc agctggtcga tcatatcgtt
    26161 gttgtccaga cggtctttgc gcacgccatc ttcatagagg ccgcttttct tgttgccgcc
    26221 ggtgacgccg agtgtagaaa ccagcgcctc acctgggcca ttcaccacgc agccgataat
    26281 cgaaacgtcc atcggagtga tgatatcttc caggcgttgc tccagcgcgt taaccgtacc
    26341 gataacatca aattcctgac gcgaacaggt cgggcaggcg atgaagttaa tccctcgcga
    26401 acggatacgc agcgatttca aaatatcgaa accgactttg atctcttcga ccggatcggc
    26461 cgccagcgat acgcgcagcg tgtcgccgat gccttccgac agcagcagac ctaaaccaat
    26521 ggcggatttt actgccccgc tgcgtgcacc accggcttcg gtgatcccca gatgcagcgg
    26581 ctgatcgatc tgttttgcca gcaaacgata agactcaaca gcgaggaaga cgtcagacgc
    26641 tttcacgctg actttgaact gatcgaagtt caggcgatcg agatgatcaa catgacgcat
    26701 ggcggattcc agcaacgcct gcggcgtcgg ttcgccatac ttttcttgca gatctttttc
    26761 cagcgatccg gcgttaacgc caatacggat cggaatgttt ttatcgcgcg cacagtcaac
    26821 caccatgcga atacgctctt cattaccgat attgccaggg ttaatacgca gacaatcgac
    26881 gccgtattcc gctactttca gcgcaatgcg atagtcgaag tggatgtcag ccaccagcgg
    26941 cacgttaacc cgctgtttga tgagtttgaa cgcttctgcc gcgtccatcg tcggtacgga
    27001 gacacggacg atatcagcgc caacgcgttc cagcgccttg atttgattga ccgttgcttc
    27061 gacatctgtc gtacgcgtat tggtcatgga ctgtacggcg atgggagcac catcgccaat
    27121 cggcacattc ccaacgtaaa tacgtgttga ttttctacgt tgaattggag cctggttatg
    27181 catgaaaaat ctcccgcgtt acccgtctgt tactgcgccg gtgattgttc ggcattgagg
    27241 gtcagacgcg caacctggtt agttctgata aaacgactca gatcgacagg tttcccttga
    27301 tactggatct gtactgcggc tggcgcacca attttcagtt tatacggtgc ctggccggtt
    27361 aaattcaaat taccgtcttt acgctgcatc ccgctaaaca attttttacc ggtagcatca
    27421 gtgacctcca gccagcaatc ggcagtaaag ttcatcacca gcgcattcgg atcagccgcc
    27481 ggcgtggtca cgccagcctg atcggttggc aacggcgcag caccatctgg cgttgttgct
    27541 gccgttggta ccggggtcgc ggcggtatca acatttgcct gcgaaggcga aacaaccgca
    27601 ttctgttgcg gatcaacagc tggtgctggc gcagttacgg caggtgtttg cgtgttggtt
    27661 gcggtagtat ccacagacgc tggcggcgtg gacgttgtag ccgggtctgt agttgtcgac
    27721 gtatttaacg ggacactctg cccctgctcg ctattactgc tcagttctgc cgaagattga
    27781 tcggccatag tggtgatctc ttcctgctga gctttgtggt cttgccacca ccaggcaccg
    27841 ctcaggccga taaccacaaa caacaccagc caggtgaagg tcatcagcca gccatcacgt
    27901 tttttgcggc gtttaccgag ggaaaaactc tgcattggcg caacttttgc agcccgtagt
    27961 ggagcctgct tttccagccc tggcagcagt tcttcttctg gaatatgtac cagacgcgca
    28021 taagagcgga tatatccgcg caggaatgtt gaagcaagat cggcgggtgc cttatcttct
    28081 tcaatgtcgc gtaccgtgga aaccttcagg caaagtcgct cggcaacggc ctgctgacta
    28141 agtcctagtt gttcgcgagc attacgcagg cgagcgccgg tagtaagtgc ttcattttgg
    28201 tcgtgcgtgg cttcagtatt cattcgctgc aggtacgttt aaatgagaat tgagatgccg
    28261 gtgaaccatc atgctcaccc acaccgcgaa acatccgtta agttaaccgt tatcatacag
    28321 tataagaccg tctgtgtgct cgtgacaaag cccacattca ctaaggctaa cttactgttg
    28381 catcgttaca tactgcctta aagtcagcaa aaacgcaccg ttaatattga ccagacaatt
    28441 gcaacttaat acttcattca taaaacatta cgccacggta cataaagtaa ccgtggcgta
    28501 atggctatca gaccgcttta atgtcgatgg cttcaccctg catccgttta cgcagggtac
    28561 gtttcgtacg gtcgataaca tcgcccgcca actgaccaca ggcagcatca atatcatcac
    28621 cacgagtttt acgcacaata gtggtgaaac cgtagctcat cagcactttt gagaaacggt
    28681 cgatacggct gttcgagctg cgtccatacg gcgcacccgg gaacgggttc cacgggatca
    28741 ggttgatctt acacggcgta tctttcagca gttccgccag ttggtgcgcg tgttcagtgc
    28801 cgtcgttaac gtggtcaagc atcacgtatt caatagtgac tcggccctga ttggcgttgg
    28861 atttctccag ataacggcgc accgcagcaa ggaacgtttc gatattgtac tttttgttga
    28921 tcggcacaat ttcgtcacga atttcgtcgt tcggcgcgtg cagggaaatt gccagtgcaa
    28981 cgtcgatcat atcgcccagt ttatccagcg ccggaactac accggaagta gaaagcgtga
    29041 cgcgacgttt agacaggcca aaaccgaaat catcaagcat gatttccatc gccggaacga
    29101 cgttgttcag gttgagcagc ggctcgccca tgcccatcat cactacgtta gtgattggcc
    29161 gctgaccggt gacttttgct gcgccgacga ttttcgccgc ccgccacacc tggccgataa
    29221 tttccgacac ccgcaggttg cggttaaagc cctgctgggc ggtggaacag aatttacact
    29281 ccagcgcaca ccccacctgc gaagagacgc agagcgtggc acggtcgtct tccgggatat
    29341 acaccgtttc gacgcgctga tcgccaacgg cgatcgccca tttaatggtg ccgtcggatg
    29401 aacgctgttc ttcaaccact tccggtgcgc ggatctccgc cacctctttc agtttgccgc
    29461 gcaacacttt gttgatgtcg gtcatctcat caaagttgtc gcagcaatag tgatacatcc
    29521 acttcatcac ctgatcggcg cggaagggtt tttcacctaa atctttaaaa aactcccgca
    29581 tctgctgacg gttgagatcc agcaggttga tttttccatc tttcgtggtg acgttttcag
    29641 gtgtgactaa ttgttcagac atatgctatt ccggcctcgt tattacacgt tatggcccct
    29701 ggagggttga aaaaagaaac gccccggtga gcggctgctc gtccgggggc gctgcattgt
    29761 acaaattctg gcgcacggat gccacgtttg cacgcggcat ttacgaaatt attaacgggt
    29821 gcgcgggcac acttcgcctt cgccgaagaa ataagcgatt tcgcgagcgg cagattcgac
    29881 ggaatcagaa ccgtgggtac cgttttcggt caggctgtca gcgtaatcag cgcgcagagt
    29941 accggccagt gcgtttgccg gattggtcgc gcccagcaga tcgcggtgac gctgaacggc
    30001 gttttcacct tccagcacgg aaaccacgat cgggccagag gtcatgaatt caaccagacc
    30061 atcaaagaac ggttttccat cgtgttcagc ataaaagcca cgagcctgtt caacggtcag
    30121 gtgcagcatt ttggtgccga caattttgaa ccctgcagct tcaaagcgcg caaagatatt
    30181 accaatgacg ttttttgcta ccgcgttcgg tttgatgatg gaaaaagtac gttcaatagc
    30241 catttttacc tctgtaaatt gttctgttgt tgtctgtacc agcgtacgag atggcgcgga
    30301 ttataatgag caacagggcc gttgactatt gatgaaggta acattttttt aaaataaaat
    30361 gagttttagc aacaatcagg gacaaaacag actattgcat gacaaatttc actgtcgcga
    30421 tttgccccac gtcatccatc accagcaatt gataatcacc tttatccgtc aaatgcaggg
    30481 tcacgttgcg cccgcgttca gttaacggtt cgccattcag aaaccaccag cgttcacctg
    30541 cccctccact tgattgcaac ggcaaagtcg cttccgctgc gcccggtaaa cgtttaataa
    30601 tcgcgccatc gcgaacgcct gtcagctgca gagggagctg ggcatcgtga ccgtatggcg
    30661 ggcaaattgt cgaggccggt ggtaagcgca cagcgcgacg ttctgatgcg ggcagccagg
    30721 gttccagcgg tagcggccag acatttacca tttcttgctt cgcctgcggg cagtcagcgg
    30781 cgacgcgctg ccctttgtca tccagccaga tggggaagcg aatgccatta atcccttcct
    30841 gctctgacag taacagcgtt ggcggctggc tgccatccag cagccaggtt gccaggcggc
    30901 ggcgacagtt accgtcacct tccggcaaag actgtccgcc cggccagcag ataacgccac
    30961 gagtgactga gttcgggcgc gggtcttccg gcagattcgc actgcgcgac agtaagatat
    31021 tattgacctg attcaacaat ggtacggcac tggcaaagcc aaactgacca acaacgggcg
    31081 tgccgtcagg tctgccagtc cagatcccaa tgacatagcg agcgttaacc ccaatcgccc
    31141 aggcgtcacg atagccatag ctggtgcccg ttttccatgc cagtggggcg acgcgcggca
    31201 aggcactatc cggcaagggt tgcgcttcat cagccataat ccggcgaatg atccacgccg
    31261 cccccgacga cattaaaggc cgttcaagca gcggatcgtc aggctgtaag cgcaatttgc
    31321 ctgccttgcc gtggcgagca aacgcggtat acgctgccgc catatcttcc agttttgcac
    31381 cagcgccgcc gagaatgagt gaaagattcg gcgcagcgcc gttgggcaaa tataacggca
    31441 atccaacatt gcgtaacttt gccgcaaacc gtttcggtcc ataggcttcc agcacctgca
    31501 cagcaggtaa gttcagcgag cgcaccagcg cctcgctcat gctgatcggg ccatgaaaac
    31561 cgctatcaaa gttacctggt cgataatcac cggtgcgccg ggggacgtct tgcagcagtg
    31621 atgccgggtg gatcaagcct tcatccagcg ccagaccata aacaaacggt ttgagcaccg
    31681 atcctggcga tcggatcgca ttgaccatat caacatgacc aaagcgtgaa tcatcgttga
    31741 gatcaaccga tcccacccag ccgcgaacac gcatatcggt atgatcaacc acgatcatcg
    31801 ccagtgagct gcgcggtggc aaccgccctt tccagttttg cgccagttct tccagacgtc
    31861 gttgaagacc ggcatccagc gtagtaacga ttttgtcgct tttgctttta ccgagcatca
    31921 tgcgcgaaaa cagcggtgcc agttgcggca tttgtcgggg tgccagccag atgggttctt
    31981 cccttgactc ttttacctgc tcacgggacc acacaccttg cgcagccatc cgttcgagca
    32041 ctttattacg cgcggcttcg gcacgctccg gccaacgatc cgggcgaaga cggctgggcg
    32101 cttgcggcaa aaccgccagc attgccgcct cggaatagct taaattcgca ggcgactttc
    32161 cgagataagc ccaacttgcc gcaccgatcc cctgcaacgt accgccaaac ggagcgcggt
    32221 taagatacaa ggtcagaatt tcacgcttag acagatgcca ttccagttgc aacgcgcgcc
    32281 agagctggcg aattttgccg ccaaatgttt tggggtgagg atcaagcaga cgagcaacct
    32341 gcatagtgag cgtgctgcca ccggaaataa cccgtcccga agtgagatct tgccatgctg
    32401 cgcgcgccac cgagaatgga ttcacccccg gatgcttcca gaaccagcga tcttcataat
    32461 tgatcagcgc ttcaaggtaa cgtggagaaa catcttcgat tgttaccgga taacgccaga
    32521 tgccgtcagc atcggcgaag cgccagagcg gcgtaccatc ctgcgccacg accactcgtg
    32581 cgggattgac ttcatgcagc ggtagaggcc agagtttatc tgctccccac gctgcaagaa
    32641 aaaggagaaa tggcgcagct gccagcgtta tccagcagcc gcgtttggtt aacaagcgag
    32701 gcatttacgg tcggacaatc agcagatctt cagccgcgcc ggtcgcccgc cattggggaa
    32761 catacattga ttccaccatc ggttgcggta cctgatacgt tcccggcgtc accgcccgcg
    32821 ccagatacac cagcgttacc ggttggtatt catcaacggc aaccgccgcc acaaagcgat
    32881 cgtcacggaa ctcaatgtgc ttaatactcg cctgctgcat ctggttcagt aagttctgca
    32941 cttcgccacc actttgctcc aggctggcgc taccgttcgc cagattctgg ttttccagtt
    33001 ccagacccac aggcagcaga tccacgacta acgcatccgg cacgctgtta ctggctttta
    33061 cctgcaacca caccagcacc agatcgccgc tacgtaacga gtccagcgat ttgctcttac
    33121 catcagtacc aagaatatga cgctcgattt gcagcacatt gttcgccggt aaaggtgcgg
    33181 attgcggata accgctggca tccatacgca accataacgg ctgatcgcca ctgttgctca
    33241 cctgcaaggt gacaagttga tcgctattca gattgctgtt ttgcgctttc tcgcctgtca
    33301 gcggctcagc tgagaaagag gtttgcgcct gccatttacc cggtaaatcc tgaatcgtac
    33361 gggcagccag gaacaacgcg ttactttcct gcgtcgatag ccagcgttca ccaaacgcct
    33421 gctgcgaaag tgtgttcagc aaagtgtact gctcatcggg tagcagttta ttttcttcca
    33481 gcaaggagag cattaacgcg ttgtcgcgca gtggactacc gtaatcaccc agccatatcc
    33541 gctcatcact atttctcggc gttttcagcg ccagcgcaat cgcttcttca ccacgcgtcg
    33601 catcacccat ggttttcagc gcaatgccaa gttgcagcag cggtaaacca gaagcggcat
    33661 ctgcacgatg ctcccagatt tcacgcagcg cacccagtgg tgctttttgc tgacgggcca
    33721 gcaccagcgc agcgtaagac tgaacggcga atttactggc tttgagatta tccgcgtacg
    33781 ggatcgacat catgcccgga tcttgtaaat agcgcagcag acgctcatta ccccggttaa
    33841 tggcgtctgt cggcacgctg taaccctgtt cgcctgcgcg gaccaggaaa tccatcacgt
    33901 aagccgtcag ccagtactct tcgtcaccgt ttttatccca cagcgcaaag ccgccgttat
    33961 cacgttgcat ttgcagcaaa cgggaaatgc cgatatcgac cgatgcacgg cgtttctcat
    34021 cactgtcgcc tttgatgccc aacgcctgca gttgggctgc gttggtataa agtgacggga
    34081 acaggccgct ggcggtttgc tcaagacagc cgtacggata cgcttttaac tctttgatat
    34141 aacgtgcaat gttcagcggt ggtttgccgc tcaacaacaa ttgcccttcc agcgtaacag
    34201 gcgagaagtt ttgcaatcca tccgccggaa tcgcccatgt ctcaccaggc tgtaacgccg
    34261 taccgtaatt aaccgtttgt gccgggaacg ccggacggac gccgattttc cactgcttat
    34321 gctgatcggc aacggtttca cccggtaacg ctaacccgct aatggtggcc tgaatttctc
    34381 catcgccata acccggcaat gctcgcaccg ggataaacag cgtagtacgc acacctggcg
    34441 ctaattcaac ggctgcgggt gaatcgctga ccagttcaag caaaccactg gcggtcaggg
    34501 caacgttcag tttttgcggt ttatcggtaa gattagtgat atccagcgtc agacgcgagg
    34561 tatcgccact cgccataaag cgcggcatgt tcagttcagc aatcaccggt gcggcaacta
    34621 tcactttgct ttcgttgcta ccgaaatcat ctgccgtcca ggcttgcgcc atgacgcgca
    34681 attcaccgtt aaaatcgcca atcggcagtg taaccgagcc ttcgccctgt tcgttgagcg
    34741 ttaccgacag cgcctgctgc gcgacaatat tgacgtgatt gaccggcggt ttaccaccac
    34801 gtttcagctc atcaccatcg ccaccgaaac gcagagctgc cagacgcccc tgaccttcaa
    34861 taacctgacc gtaaatatcg taaatgtctg cgccatagcg tttctgacca aagaacgcct
    34921 gccacggatc tggcgtaacg tagtcagtaa tattcagcac accactatca acggcagaca
    34981 ccagcacatt cacctgttta ggcttctcgc catttttagt gctggcttta attttcacgg
    35041 ttaatggttg attgggacgc atttttgctg gtgtttccag cgccagatcg aggcgacggt
    35101 tttcatcacc aagcggcaga tgcaacacac caaccgcgcg ttttggcgtc gcggagcgag
    35161 atttatcgcc aggacgcacc accagcgtac tcaaataaag atcatgacga ttccaggttt
    35221 tatcgaccgg aatcgtcaga tccagccctt gagccggaac atcaatctct tgccaccaca
    35281 gcggcccttc actggactcg accatcgcat aacctttacc cgccgttggc gcggcaatat
    35341 gcaacttaat ggtatcgccg gggcgataac tggctttatc tagtttcagc gtgacacggt
    35401 cgggtcgcac tgcgccgctc ccgtcgctgt tgtcctgcca gctatagcca gcccaaaaac
    35461 gcacgctact gaccgcttca ttcggcgctt tgacttccag acgataagcg ccccactcta
    35521 ccgggaaact gaccttaccg gtttcatccg cctgaagatc cagagtttgt tcattttcga
    35581 tcagatcttt ttgatcaaac tgtgactgcc agccttcatc ttctgaccag ttccagtagt
    35641 aatcgcggcg ttcgcgaatc aggcgcacct gcaagcccga cacggctttt ttcacgcctt
    35701 gcgcatcgct ataaacgatg tcaaaagcgg cgttactgcc ttcatcaaca atgggctgtt
    35761 tcaccgtgct gtcagtgcga taatcgtaaa ccgatttcaa ggcgaactgc ggacggagcc
    35821 ccggcaatgc atcggcaggc cagatagcct gctcagcgcg gcgcgtcacc gggcgaccgc
    35881 ccgattccag caaactgccc tggaaaataa cctgcaatgg ggaatgcgtt tccttccact
    35941 ggctttctgt agaaacttcg ccgcgccctt tatcatccag cgtcaactga acttcatcca
    36001 gcgtgcggga aaggttttcg gcagctatat cgccgaattc aaaaccaggt aaggctgaca
    36061 cagcctcacg cagtgggcgc aggaaaagtt gcccttgcaa agtattacca ttagcaggcg
    36121 caccgtacag gtagtatccc accacggaga atttcacttc atcattcggc gtaagcgggg
    36181 ttttctcacc ggtcagattc agcgccatac gctctggcat aaaatcttcg acgtggaaat
    36241 cccacatccg gtactggtta tcgcccgtgt tggcgcgaat atgccacata ccggttgccg
    36301 cattgctatc gagtggccag gtaaagtggt agaggccatt ctccggctga ctaacgacgt
    36361 tcctgagtac ctgcccgtca ggtttaatca cgtctaactt gatgggttga tcgggcaatg
    36421 ctttaccgtc tgcatcgcgc agtaaaccat tgaggattac cgtttcacct gggcgataaa
    36481 gatcgcgtgg gccaaacatg aaaaactgtt tgctatagcc tggcgcgcca gcaatgttaa
    36541 attctgctaa gtccagcgcc ggaagtttta aatcgagtag cgttgtctga ccgtctttac
    36601 gtgccagcag taatgccgca tttttatcat tttccagctg cacatgcccc tgagcgtcac
    36661 tggttgcctg agtcagagtc tgtcctttct catttaataa agagacttca attccttgct
    36721 gggccgcgcc gttttccaga ctttgggtaa agatatccag acgattgtga taacggtgag
    36781 ctgaaacgcc gatatcactt aacgtaaaca gcgtcgcggg attactgtaa tcgtaacgtc
    36841 cagcctgatt catcacagcc agatacacgc ccgcctgctg aagcggtttg atatcgccca
    36901 gcggcagcaa taatttttca cgggtgttac gcgcaggatt gagatcaaac cgtccggtgt
    36961 agaccagatc cgccatctgc agcagtttgt ctgactgcca gttcgccagc gaattgcggt
    37021 attcccattg gctaatgaat gctggcagag attctggctt aacgcggaag aagttaacat
    37081 cgacattatt aacgttgagc gccattaccg gcagcccttc aacgactttg ccaggcagca
    37141 gcgaaccacg gctggcaaaa ccgacgctgg gttggatgtc gcgggtagtt atagtttttt
    37201 cgtaatcttt actgaaggtt gcgttgttga gtgctttgac ctccttgcca atagtaacga
    37261 tcaaatcacg tttcggttcg aggtggcgta aacgtagctc tttaagatta tctgacagct
    37321 cccaggcacc atccactttg ccgctttttt tatcgacgac atgaataacg cgtgagaaat
    37381 cctgatccgg gtcgagaggg atggagaacg tcagcaccag cgtggctgca ccatcaagtt
    37441 ggacttcaga gagatccagc aatgtcagca ccttaccggc actctgttgg gccagttttt
    37501 gtctctccgg cacggagagt tttgctgaac tcgcgttttc tgaagaggcc gctttagtaa
    37561 cttcagaagg cgcatctttt ttcaccgctg ttggcgcgtt atcgttgttg tcgcaccctg
    37621 ccagcgccag cattagcatg caggcggcta cgcgtaactt tttcattttt catccctgcc
    37681 acaatggccc gttagcaacg tcgaataatt attatgcgtg agaatttacg ttatgtaatt
    37741 cagtatcacg cccatacaat ccgaatttta gtgagaattg tggctcagac agccaaatca
    37801 gcctctttcg cttgtcgctt ttcactaaac atccgacaat ttagcctaac cccggcaaaa
    37861 atggagatgc ctatgtccac gacatggttt gtaggagccg actggctcgc cgaacatatt
    37921 gatgacccgg aaattcagat tatcgatgcc cgcatggcgt cgcctggaca ggaagatcgt
    37981 aacgttgctc aggagtatct gaatggacat attcccggcg cagtgttttt tgatatcgaa
    38041 gcgctttctg atcacacttc cccgcttccg cacatgctgc cgcgcccgga aacgttcgcc
    38101 gtggcgatgc gtgaattagg cgttaaccag gataagcacc tgattgtcta tgacgaaggt
    38161 aatcttttct cagccccacg agcatggtgg atgctgcgca cctttggtgt agagaaagtg
    38221 tcgattctgg ggggtggact tgcaggctgg cagcgcgatg atctgctgtt agaagaaggt
    38281 gcagtagagc tgccggaagg agagtttaac gccgcgttta atcctgaagc cgtggtgaaa
    38341 gtaaccgatg tattattggc aagccatgaa aatacggcgc aaattattga tgcccgcccg
    38401 gctgcacgtt ttaacgcaga agttgatgaa cctcgcccag gtttacgtcg cggacatatt
    38461 cccggagcac tgaatgttcc gtggacggaa ctggtgcgcg aaggcgaact aaaaacgacc
    38521 gatgaactgg atgcgatatt ttttggtcgc ggcgtcagct acgacaaacc aattatcgtc
    38581 agctgcggct ctggtgtaac ggcagccgtg gttttgttag cactcgcgac gctggatgtg
    38641 ccaaacgtga aactgtacga cggcgcatgg agtgaatggg gcgcgcgggc agatttaccg
    38701 gttgagccag tgaaataagt attttacagg caataaaaaa ccgccgaatt tggcggtttt
    38761 ttattgctgg tctggttcgc ggcctttcca gcaggttgta ttaccgtagt aatgcaagcg
    38821 cgtctcagcg gagacaatac tcgccagtaa ctctcttttt gtcaagcaaa agagagttat
    38881 tattgttctg ttagtgtatt atccactgcg gccctttccg ccgtctcgca agcgggcgct
    38941 ggcttaagga aaggatgttc cgcgaccgtt agagcaagcg cgtctcagcg cttgctatcg
    39001 cggcaatatc gccagtggtg ctgtcgtgat gcggtcttcg catggaccgc acaatgaaga
    39061 tacggtgctt ctgtatcgta cttattgttt ctggtgcgct gttttcccaa gtaaataata
    39121 accggagtct ctccggcgac aatttactgg tggttaacaa ccttcagagc agcaagtaag
    39181 cccgaatgcc gccctttagg gcggcatatt ttagattatc cggttttgtt taaagtcacg
    39241 caaaaaacca ccccagcgac gttcatagaa tggcacaata tgttcggtaa taaagtggct
    39301 aattcctttt tccccttttt tcacctgaca aatatcgatt ggttcatcgc caggtaatgt
    39361 atcggtcgct acacttcccg tcgcctgaat aatttcttcg atatcaccat cggcttcaat
    39421 gccaataagt aaattaggct gtgcctcttc gttctcttta attgaacaaa taaaagcacg
    39481 cttcaccggc ttaatggttt taaataaggt ggtgagtgaa tcaatcattt gtgctggcgg
    39541 ctctgcgact tccgataata tcagcgattc accgccttcc aggatttcct ggctgctcag
    39601 cggatttccc tcttcaccaa tcaacaaact gatttcacgc ggcataaatt ctttaccggt
    39661 cggcagtttg gcattaagga agagcgtttc gccaagggtc atctcaaaca atgtgcgaac
    39721 gggcattacg acaaatgcct gttcgtcttc aaccgcctgt tgaagcgctt ctaacgaggt
    39781 gaaaaaagga atgacgctgg tgccgtcttc tttttcccag tgctgtaaat caagcgcact
    39841 atcttcaacc acagcctcgc cctgcgccgc cgtaccaggc acccagacgg tggattccag
    39901 tagagtacgg aaaaaggccg ggcggtgcgc cggttcggtt gctgcttttt ccagcaggtc
    39961 ttcaagttcg ttttttgttt cggacataag aaccacaatt cattcaacgt tcggggcgca
    40021 aatgcctgat gcgctacgct tatcaggcct acaagggatt cgcaatttgt tgaatttgca
    40081 gaatttgtag gccggataag gcgtttacgc cgcatccggc atattagttt acgccgttaa
    40141 cagattagct atcgtgcgca caccaagtcc cgtagcgccc gcagaccact gttcaaccgg
    40201 cgctttacgg taagtcgccg agcagtcgat atgcagccag ccttgctgat agttctcaac
    40261 aaagtgcgac aggaagcccg ccgccgtgct cgcgcctgcc ggatacgccg cgcttccggt
    40321 attgttcagt tcggcaaagt tagacggcag ctggctgcgg tggaattccg ccagcggcag
    40381 acgccagaat ggttcgttct cttgtgaggc actcgccagc aaacgaccgg caagcgcatc
    40441 gtcaaaactg aacagcgcgt gataatcatt acccagcgca gttttcgccg ccccggtgag
    40501 ggtcgccgca tcaatgatca tttccggttt ctgcgcactg gcatcaatca gaccatcggc
    40561 aagcaccaga cgcccttcgg catcagtgtt catcacttca acttttttac cgttgcgata
    40621 ggtgatgata tcgcccagct tgaacgcatt gccgctaatc aggttatccg cacagcagag
    40681 gaacagcttc acgcgcttgt tcagtccgcg cgtaatggca aatgccagcg ccccggtaac
    40741 cgttgccgcg ccgcccatgt ccgacttcat cgagtccata aacgcagtct gtttgatgct
    40801 gtagccgccg gagtcaaaag tgataccttt acctaccagg cacgcgtaca ctggcgcttc
    40861 tttatcgcca gttgggttgt aatccagcgc cagcaatacc ggagaacgtt ctgaaccgcg
    40921 tccgacggtg tgcagcccca tataaccttg ctcacgcaga tcttcgcctt tggtgatccg
    40981 ataagtcaca cgatcgcccg cgacgttgct gatcagatca acagcacgct gtgccagttg
    41041 cgatggtccc aattcttctg ccggtgcgtt gatggtgtca cgcacccagt cgatgatcat
    41101 caggcggtta tccagttcct ggcgctgggc atcgtccaga tccggccact ccactttacg
    41161 cgtgcctttc ggggctttgt aaccttgcca gaatgcccag cagcgatccg catcccagcc
    41221 ttcaccgctt aactgaacat gcttgatgcc cagaccgtca atcttgcgcg ccgcacgctg
    41281 gatcagcccc agatcgtctg ccccgttcaa atgcagggta atgccgtcat tattaatgct
    41341 gtaagttgct ttttctcccc agcgcgcgtc ggcaggttgg gtagagaggg taatcttcat
    41401 cgcttctgtc attttagtta tccttctttg taaaagggcc gccagttggc agccccagga
    41461 tttttccgct aaatgattcg cgttgcagaa aggcggcaag tgagtgaagc cccaggagca
    41521 tagataacta tgtgactggg gtgaacgagc gcagccaacg catctgcggc gtgaagcatg
    41581 acgcggaaat tattcggcct cgtccagcca gactaacaaa atcgcttcga ggattttctc
    41641 gttggatgcc tgcgggtcgt cgtcgaaatc ttccagatcg caaatccact gatgcatatc
    41701 ggtgaatcga accgttttcg gatcaagatc aggatacgca tcgtacagtg cttcgccaat
    41761 ttcgcggcta tcggtccact taagtcccat actaacctct gttaatgctc acgcgcatgg
    41821 ttgatagtgt aacgcgggat ttcgactact aaatcttcgt cggtaacgcg tgcctggcag
    41881 cttaaacggc tttcgggctc cagtccccag gctttgtcca gcatgtcgtc ttcctgctct
    41941 gagctttctg gcagcgagtc aaaaccttca cgaacgatgc agtggcaggt ggtgcaagca
    42001 caggattttt cacaggcgtg ttcaatctcg ataccgttac gcagagctgc gtcgagaatg
    42061 gtttcaccgc tattagcttc cagaacagcg ccatcaggac agagatcctg atgaggcaaa
    42121 ataacaatct ttggcatatt aaacctcgtc cacggaatgg cctttcagcg cacgacgaac
    42181 cgactggtcc atgcggcgag cggcgaaatc ctgggtttgt ttgtctacgt ttttaatcgc
    42241 ttgttcgatg gcgtcaacgt catcgccctg cgccacttca ctcaggtgag cggcagcatc
    42301 gtcaatgacc tgacgttctg cggcgcttaa cagcgcggca tcagcagcca gcgcgccgtg
    42361 cagactttcc agcacacgcg ccgcttctac tttttgttct gccagcattc gggcttttac
    42421 gtcctgctcg gcatagctca ttgagtcttt gatcatcgaa gcgatttcgc tatcggtcag
    42481 accgtaagac ggtttgacct gaatagacgc ctcaacgccg gtggatttct ccatcgccgt
    42541 cacgctcaaa agaccgtcgg catcgacctg gaacgtcacg cgaatatgcg caccaccagc
    42601 cggtagcgcc ggaataccac gcagcgcaaa acgcgccagt gagcggcagt cctgcaccag
    42661 ttcgcgctca ccctgcatta catggataga catcgccgtc tgaccatctt taaaggtggt
    42721 gaaatcctga gcgcgggcca ccggaatagt ggtattacgc ggaatcactt tctccaccag
    42781 gccgcccatc gtttcgaggc ccagcgacag tgggatcaca tcaagcagca gcatttcgct
    42841 gtctggcttg ttacccacca gaatatccgc ctgaatcgcc gcgccaatag cgacgacttt
    42901 atccgggtcg atggaagtca gcggtggacg accgaaaaat tcgcctaccc gttcacgcac
    42961 cagcggcacg cgagtagaac cgcccaccat caccacttcc agcacttcgt cagcttctac
    43021 acccgcgtct ttaagcgcgc gacgacaagc cagtaaggtt cgttttacca gtggcgcgat
    43081 cagttcattg aattgttcac ggctgatttc gccctgccag cccgcaacgt taacggtcac
    43141 ggagtccgca tcgctcagcg cgattttggc tgcaatggcg gcatccagca gttcacgctg
    43201 aacgcggtta tcgctacgat caggaatgcc cgcctgctcg cgaatgtaat ccgccagcag
    43261 atggtcgaaa tcatcgccgc cgagcgcgga atcaccgccg gttgccagca cttcaaacac
    43321 gccgcgactt aagcgcagaa tggaaatatc aaacgtcccg ccaccgaggt cataaacagc
    43381 gatcacgcct tcctgaccgg aatccagccc gtaggcgata gccgcagcgg tcggttcgtt
    43441 aagtaagcgc agaacatgca accccgccag acgcgccgcg tctttggtgc cctgacgctg
    43501 ggcatcgtca aagtacgccg gaacggtgat aactacacca tccagctcgc ctgccagggc
    43561 ttcagttgcc cgcgccgcca gtgctttgag gatgtccgca gaaacgcgca ccgggttcag
    43621 cagccccgcc gccgtttcaa tcatcggcag gccgttttcg ctggcctgga attgataagg
    43681 cagatgcgga tagcgttgct ggatatcagc cagcgagcgc cccatcaggc gtttaacaga
    43741 actaatggtg ttggcggtgt caagcgctgc gttagtacgc gcgtcataac ccaccgaatg
    43801 cccttgctgt tgatagtgaa caacagatgg cagcaggtga cggccttcat ggtcggctaa
    43861 cgtttcggcc tgaccgctgc gcactgtcgc caccagcgag ttggttgtgc ccaggtcaat
    43921 accagccgcc agacgacgct gatgcggcgc agcactcaaa ccaggttcac taatttgtaa
    43981 taaggccatg tttagcttcc agaaattaaa aatcgagcag tttttcttcg agttgttcgg
    44041 cactgctccg cagtttatcg agaaaacgca gcttacgcac ggtatccgcc gccgcgtccc
    44101 acgcctcgtt gtctaactgt tcaaccatca actgatggcg ggtatcaaac atctttttca
    44161 cacgtttgat aaagctttcc agccgcgctt catctttcgc ctgttcgatc tcgtccagct
    44221 cttcgcgcag ctccaactgt tccatcagga acgcggtgtc gcgcacagta tgctgctcgc
    44281 tggcgagatc aaaaccgtgt aaagaaagca aatattccgc gcgcattaac ggatgacgca
    44341 gcgtttgcca ggcctggtta atggttgcag attgctgtac ggcggcgagt tgttccgcct
    44401 ggcttccgct ggcgaattta tcaggatgat actgacgttg tagatcctga aaacgcaggc
    44461 tcagcgcctg ggtatcgagt tgataacggg caggcaagcc aaagaaggtg aagtaatcca
    44521 taacattctc agggctgcga tccgccgcga taacggcgaa tcaggtaaaa caaaccccac
    44581 gcgcaggcga ccacggtggg gttatcggta tgcgcatcaa acgtggaagc tttcgccgca
    44641 accacactca tctttgacgt tcgggttggt gaatttaaac ccttcgttca ggccttcttt
    44701 tacgaagtcc agctgcgtac cgtccagaaa ttgcaggctt ttgccatcga ccacgacttt
    44761 cacgcctttg tcttcaaaca cgatgtcttc cggcgtcggt tcgtcaacaa attccagtac
    44821 ataagccata cctgaacatc cggaggttct cacgcccaga cgcaggccaa accctttgcc
    44881 gcggttagcc agaaaggtat ttactcgcgc tgctgcactg tcgctcagtg taatcgacat
    44941 aaccaaacct caactcttat tttgcttcac gtttgctttt atagtccgca atggcggctt
    45001 tgatcgcgtc ttctgccaga atagaacagt gaattttcac cggcggcagt tcaagttctt
    45061 cagcaatatc ggtatttttg atcgcctgcg cttcgtcgag agacttccct ttcacccatt
    45121 cggtgaccag ggagctggaa gcgatagcag aaccgcagcc gtaagtttta aaacgcgcgt
    45181 cttcaatgat accttcatcg ttgactttaa tctgcaactt catcacgtcg ccacaggccg
    45241 gtgcccccac catgccgctg ccgacgttct cgtcgttgtt gtcaaaggaa cccacgttac
    45301 gcggattctc gtaatggtcg ataacttttt cgctgtaagc cattataaat tctcctgatt
    45361 ccgataccga ttaatgatga gcccattcga tgctgttcag atccacgccc tgcttgtaca
    45421 tttcccacag cggagaaagg tcacgcagac gaccgatgga tttacgaact aactcgatgg
    45481 tgtagtcgat ctcttcttca gtagtaaaac gacctaaaga gaaacggata gagctatgtg
    45541 ccagctcgtc gttcagcccc agcgcgcgca gcacgtagga cggttcgagg cttgctgacg
    45601 tacaggcgga acctgaagaa actgcgaggt ctttcagcgc cataatcagc gactcacctt
    45661 caacgtagtt gaagctgacg ttgagaatgt tcggcgcacc gtgttccagg tcaccgttca
    45721 ggtaaacttc ttcgatatct ttgatgccgt tccacagacg gttacgcagg ccgcgcagac
    45781 gttccatctc ggtcgccatc tcttcttttg cgatgcgata ggcctcgccc attccgacga
    45841 tctggtgaac aggcagagtg ccggaacgca taccgcgctc gtgaccgccg ccgtgcattt
    45901 gcgcttcgat gcgtacgcgc ggtttacgac gtacatacag cgcaccgata cctttcgggc
    45961 catagatttt gtgaccggag aaagacatca ggtcaacttt caactggctc aggtcgatag
    46021 gcagtttgcc cacgctctgg gttgcatcaa cgtgatagat aatgccacga gcacggcaca
    46081 tttcgccgat agccgcgata tcctgcacca cgccgatttc gttatttacg tgcatgatgg
    46141 aaacaaggat ggtgtcatca cgcatcgctg cttcaagttc tttcagatcg ataatgccgt
    46201 tacgctgcgg tgccaggtag gtgacttcaa aaccttcgcg ctccagctga cggcaggtat
    46261 ccagtaccgc tttgtgttcg gttttgctgg tgatgatgtg cttgcctttt ttctgataaa
    46321 agttggctgc acctttgatc gccaggttgt cagattcggt tgcaccagag gtaaagacga
    46381 tttcacgcgg atcagcgccg accagatcgg caatctgatt acgggcgata tctaccgctt
    46441 cttcagcctg ccagccgaaa cggtgagaac gggaggccgg gttaccaaag gttccgtcca
    46501 tcgtcataaa ctgcatcatt ttctcggcaa cacgcgggtc caccggcgtg gttgcggagt
    46561 agtcgagata aatcggtaat ttcattgctc tataaactcc gtacatcact caatgcaagg
    46621 aaccaggcta ccggctggat gtacgaccgt gtttacggag cgcgatacgc cccggcctga
    46681 ttctaatatc ttgttatctt taacctaact gtttcgggtt tcagcaagtt gacaaactta
    46741 cgaatctccg ggagcttaca taagtaagtg accggagtga gtatgtgcag tcaacgcaga
    46801 tgcaacacga aacaggacgg ttaaattaag cgcgtaactt aacgtcgatc gcgtcttgta
    46861 tgcgggtgcg tggcgcgtcg tgagtatgct gacgaccaga cacatccagc acttcctggt
    46921 tattaaccag ttcgcctaaa gtaatgttgt tgagaaaacc ggtgagacgg tcgctcaaat
    46981 cacgccacag cgcgtgggtc aggcatttat cgccgccctg gcaaccgcct ttaccctgac
    47041 aacgggtagc atctacagat tcgtcaacgg cgctaatcac ttcgccaacg gcgatgctgc
    47101 tggcatcttt gcctaacaga taaccaccgc ctggtccacg tacgctggaa accagaccat
    47161 ttttacgcag acgggaaaac agttgttcca gataagaaag ggaaattccc tgacgttcgg
    47221 aaatatcagc caacggtacc gggcccgctt cagagttgag cgcaacgtca agcattgcgg
    47281 tcacggcata gcgcccttta gatgtcagtc tcatgtctta cttcacctca aactcgcccc
    47341 tgcccggggt tttttattgt aaaagtgggg gtattgcata gcagggtcaa gtctgacatt
    47401 cccgagtaaa ttggtcaact atttacttga ctgatttagt cgggtattta accttcagtg
    47461 ccattttttt atcgtggcgt ttgtacctgt tgtccttcca gaccatgggc gggttgaggg
    47521 caattattcg cattagcgct ggcctcttcc cctcacccta accctctccc cgaaggggcg
    47581 aggggaccgt ccgtgcattt atctggctta gccctcaggc cttattacct ttattctgct
    47641 gctctataga agccagaatc ccgcgcagga tattcaattc ctggctttcc gggcgcgcac
    47701 gggtaaacag acggcgcaat ttattcatca cctgccccgg atggttttca cggataaaac
    47761 cggttgccag cagcgtttgt tccagatgac cataaaaacg ctccagatca tcgaccagcg
    47821 gatacggcgt ctcttcatgc tcgacctgct cgccattttc ctgagttgcc agccaggcca
    47881 tacgcacttc ataggcaata acctgaaccg ccattgccaa gttcagcgaa ctgtattccg
    47941 ggtttgccgc aatcgcgaca tgataatggc atttctgcaa ctcttcattg gtcaagccga
    48001 cgcgctcacg accaaacacc agcgccaccg gggtatttgc cgcttcagcg acgcttttca
    48061 gaccgcattc gcgcgggtcg agcatcggcc acggcagcgt gcgggagcgt gcgctggtgc
    48121 ccaccaccag gctacaaccg gctaacgctt cgtcgagcgt atcgacgatg tgagcattac
    48181 caatcacatc gctggcccct gctgccaggg caatcgcctg ggagtcgggt ttcaccagtg
    48241 gattaaccag ccacagattg gttaatccca ttgttttcat ggcacgggca acagaaccca
    48301 tattgccggt gtgtgacgtc tccaccagca caattcgaat attttgcagc attgtctttc
    48361 ttcatctaaa gattattcac gcatcttatc ataaaacgaa gacagatgcc gatctcgctg
    48421 ctatactctg cgccgttttc ccgttcttta acatccagtg agagagaccg atgcatccga
    48481 tgctgaacat cgccgtgcgc gcagcgcgca aggcgggtaa tttaattgcc aaaaactatg
    48541 aaaccccgga cgctgtagaa gcgagccaga aaggcagtaa cgatttcgtg accaacgtag
    48601 ataaagctgc cgaagcggtg attatcgaca cgattcgtaa atcttactca cagcacacca
    48661 tcatcaccga agaaagcggt gaacttgaag gtactgatca ggatgttcaa tgggttatcg
    48721 atccactgga tggcactacc aactttatca aacgtctgcc gcacttcgcg gtatctatcg
    48781 ccgttcgtat caaaggccgc accgaagttg ctgtggtata cgatcctatg cgtaacgaac
    48841 tgttcaccgc cactcgcggt cagggcgcac agctgaacgg ctaccgtctg cgcggtagca
    48901 ccgctcgcga tctcgacggt accattctgg cgaccggctt cccgttcaaa gcaaaacagt
    48961 acgccactac ctacatcaac atcgtcggca aactgttcaa cgaatgtgca gacttccgtc
    49021 gtaccggttc tgcggcgctg gatctggctt acgtcgctgc gggtcgtgtt gacggtttct
    49081 ttgaaatcgg tctgcgtccg tgggatttcg cggcaggcga gctgctggtt cgtgaagcag
    49141 gcggcatcgt cagcgacttc accggtggtc ataactacat gctgaccggt aacatcgttg
    49201 ctggtaaccc gcgcgttgtg aaagccatgc tggcgaacat gcgtgacgag ttaagcgacg
    49261 ctctgaagcg ttaatgactc aggcgggtga tgtcactcac ccgccctcgc ctttcaggcg
    49321 ctattctgaa atacttcctc accgctttac tttctttcgt cactctccca ccatctttcc
    49381 ccgattaatg gatgataaag aagtcacaag gaaaggttat ggcgctgcca gtgaacaaac
    49441 gcgttctcaa aattctgttt attctctttg ttgttgcctt ctgcgtttat ttagtgccgc
    49501 gcgttgccat caacttcttc tattatcctg acgataaaat ttacggtccc gacccctggt
    49561 cggcggaatc cgtcgaattt acggctaagg acggtactcg tctgcaaggc tggtttattc
    49621 cttcttcgac gggccctgct gacaatgcca tcgcaaccat cattcatgct cacggcaatg
    49681 ccggaaatat gtccgcccac tggccgctgg tcagttggtt acccgagcgt aatttcaacg
    49741 tttttatgtt tgattatcgc gggtttggta aatcaaaagg cacgccgtcc caggccggat
    49801 tgctggacga tacgcaaagt gccatcaatg tggtgcgcca tcgcagtgat gtaaacccac
    49861 aacggctggt gctgttcggg cagagtattg gcggggcgaa tattcttgct gttattggtc
    49921 aggatgatcg tgaaggcata cgtgcggtga tcctcgactc cacatttgcc tcttatgcaa
    49981 ccatcgccaa tcagatgatc cccggcagtg gctacttact tgatgagagt tacagcggcg
    50041 aaaattatat cgccagcgtc agcccgatcc cgcttttact cattcacggt aaagctgatc
    50101 acgttatccc atggcagcac agcgaaaagt tgtatagcct ggcaaaagag acaaaacggt
    50161 tgatcctaat cccggatggc gaacacattg atgctttttc cgatcgtcac ggcgatgttt
    50221 atcgcgaaca gatggtggac tttatcctta gcgcgttgaa tccgcagaac taacccatga
    50281 tcgctagcac gataatcatt cacaaaacca ccttaagaca tgctaatcca ctggtcagaa
    50341 cagtttaaga tgagaaaaat tctgtgacgc ttgccaacat ttctgatgat tagcattccc
    50401 ttcgccatct ccctgagcaa actttagcta ttcttatcaa ttatgcttat gggagatcta
    50461 cagatgatgc ctacgcttgc tccaccatct gtcctttcgg ctccccagcg ccgctgccag
    50521 atcttgctga cgctctttca gccggggttg accgccacca tggcaacctt cagcgagctt
    50581 aatggtgtgg atgatgatat tgccagtctt gatatcagcg aaacaggacg ggagatcctg
    50641 cgctatcatc aactcacact gacgactggt tatgacggta gctaccgggt tgaaggtaca
    50701 gtgcttaacc aacgtttgtg tttatttcac tggctacgac gtggtttccg tctgtgtccg
    50761 tcatttatta ccagccaatt cacccccgcc ctgaagagtg aactgaagcg gcgcggaatt
    50821 gcgcgtaact tttacgacga taccaatcta caagcgttag tgaatctctg ctcccgacgg
    50881 ctgcaaaaac gctttgaaac gcgcgatatt catttcctgt gtctgtatct gcaatattgt
    50941 ttgctgcaac accacgctgg aattacgcct cagtttaatc cgctccaacg tcgctgggcg
    51001 gaatcctgcc ttgaatttca ggtagcgcag gaaattggac gccactggca gcgtcgggcg
    51061 ctccagcctg taccgcctga tgagccactg tttatggcat tacttttttc catgttgcgg
    51121 gttcccgatc cattgcgtga tgcgcatcag cgagacagac aattgcgtca gtctatcaaa
    51181 cgtctggtaa accattttcg tgagctggga aatgttcgtt tttatgatga acaggggtta
    51241 tgcgatcagc tttataccca cctcgcccag gcgttaaatc gcagtttgtt tgccatcggt
    51301 attgataata ccctgccgga agagttcgcc agactgtacc cacgcctggt gcgcaccacc
    51361 cgcgcggcgc tggccggatt tgaaagtgaa tacggcgtcc atctttctga tgaggaaagc
    51421 ggtctggtcg cggtgatttt cggtgcctgg ctaatgcagg aaaacgatct gcatgaaaaa
    51481 cagattattc tactgaccgg gaatgatagc gagcgagaag cgcagattga gcagcagtta
    51541 cgcgaactaa cgttactgcc gctcaacatt aagcatatgt cggtaaaggc atttttgcag
    51601 acaggcgctc cgcgtggcgc ggcactgatt attgcgcctt ataccatgcc attaccgctc
    51661 ttttcaccac cgctgatcta tacggacctg acgctgacaa cacatcaaca ggagcagatc
    51721 cgcaaaatgc tcgaatcagc atgagggaac aactttcggg cgcaaaaaca ttgccggaag
    51781 cgccaccagc gccattaccc agaacacgcc gtggcccaga tattgataca ggaaaccggc
    51841 gaaaacggtc atgatagcga tactgccgcc catcgcgacg gcagagtaaa ccgcctgtaa
    51901 acggatgact tcgctaccct ggcgaacagc aatatagcgc atggcggcca ggtggcagac
    51961 cgtgaaggtg ccgcaatgca aaatttgcac cactatcaac cacggcaacg ccgtagttgc
    52021 tcccataatg ccccagcgca ctacgccgca aatcgccgag atcaacagca tatcgcgagc
    52081 actacaacgg cggaaaagtt tattactcag cgcaaagata atgacttccg ccaccacgcc
    52141 cagcgaccac aaatacccca ccgccgaggc cgagtagcca gctgcctgcc agtaaatggc
    52201 gctaaaaccg taataggccg aatgtgcccc ctgcaataaa caaacgcagg ccagaaagcg
    52261 ccagttctgg cgaaccagcg ccaaccacgc agaccaaccg gtgctctcct gctggcgact
    52321 tgccccttgt ggctgaatcg tcggacggat gagaaagccg agcagcatgg atgccacgcc
    52381 caacgtcaac agcgcgagga tcacccgata atcaaacata gtgaccagtt tgcccgtcag
    52441 cgccgagcca atgacaaacg ccaccgagcc ccacagtcgc actttgccgt aatcaagcgg
    52501 gaactgcttt tgccacgtat tcgccagtgc atcggtcaac ggtaccagcg gtgagaaaaa
    52561 gaggttaaag ccaatcatca ccagcatcag ccacgctacg tgcgcccccg cccagaaggc
    52621 gacagcaaag agaagtgtca gcagtgccag cacgcgcaag gcggaaatca ggcgggaagg
    52681 atcgctgacg cggggcgcga tgagcaaact cccgagaaaa cgggcaacca gacctgcccc
    52741 caataacagg ccgatggttt ctggcgttaa accaatccct ttaagccaga cgctccagaa
    52801 aggtagaaaa atgccgtaac taaaaaagta tgtgaaatag ccgagcgcca accagcgcgt
    52861 ggattgcaaa accatgagtc cctcccgtca ggaggcgtta gtctggggtt aatcgaatgt
    52921 tggtgcaagt tgaaaaataa gaatattcga gcctgcttcc cgatgtctgt aacgccagtt
    52981 cacaagcact aaaaaactgc gatccgcgac gcatgttgca aaaataatgc gtaattatgc
    53041 cgttacgctt gccaaacgtt cctgcacaat ggcgatgaaa tcgcgtaatg ccggcttcat
    53101 ctcccctttc ttccacgcca tcagtaaagc aatggaaggc acatttccgg caatgggacg
    53161 aaaaacaacc tgcccggtat taaaattatt catataaccg ggtatcaaag tgacgcccag
    53221 ccccatgccc accagattca tggtcaccag aatattcgtt gccacctgga cgatatttgg
    53281 ctggctgttt tcttgcgcaa accacgcttt aacgatcggc gcaagcgaac cggaatacgc
    53341 cggatcggta ctgacgaaat tcacgccatc cagttgcgcg gcggtgatct ctttttcatg
    53401 tgctaaaggg tgatcaaccg gtaacaccac cactaatggt tcgtcaaaaa gctccagata
    53461 atcaatctcc gggctataaa caggatggcg cattaagccg acgtcgagtt caccacggcg
    53521 aattttttcc tcctgttgcg tggtgattaa actcaccagc tcaatcaagg tgtctggctg
    53581 tctgagacga aacattggta atacttttgg caataaattc acttccgccg atggcacaaa
    53641 gccaatggtt aattgtctgt cttcctgaac aattttccgc gcccgtaatt tggcattttc
    53701 cgcttgttcg aggattgcca gcgcgtcctg gagaaaacat tctcccgccg ccgtcaacgc
    53761 gactttgcgc ttatccctca ccagtaacgg aacaccgaca cagttttcaa gatcgcggat
    53821 ctggctgctt aacgaaggct gtgaggtatg cagtttttcc gccgcacggg taaagttcag
    53881 tgcctgcgcc actgcgacga aatagcgtaa atgccgtagt tccatcacct tccccttgtt
    53941 atcgaaaaaa cgtctcagct ggtagaaatt aactatttca catattagca accaaccagc
    54001 aacatcctta tgacacaaaa atagaaggtc aatacatctt atctttcagg attaaaaaat
    54061 atgaccacac cctcagattt aaacatttac caactgattg atacccaaaa tggtcgggtc
    54121 actccgcgta tttataccga cccagatatt taccaactgg agcttgaacg tattttcggc
    54181 cgttgctggt tatttctcgc ccacgaaagc cagatcccaa aacctggtga tttctttaac
    54241 acctacatgg gggaagatgc ggttgtcgta gtgcgtcaga aagacggcag cattaaggcg
    54301 tttctcaacc aatgccgcca ccgggccatg cgtgtgagtt atgcagattg cggcaacact
    54361 cgcgccttta cctgtccgta tcacggctgg tcttatggca ttaacggcga gttgatcgat
    54421 gtaccgctgg aacctcgcgc ctacccacaa gggttgtgta aatcccactg gggactaaac
    54481 gaagttcctt gtgtggagag ttataaaggg ctgatttttg gcaactggga taccagcgca
    54541 ccgggcctgc gtgattacct gggtgacatt gcctggtatc tggatggcat gctggatcgt
    54601 cgcgaaggcg gcaccgaaat tgtcggcggc gtacaaaagt gggtgatcaa ctgtaactgg
    54661 aaattcccgg cagagcagtt cgccagtgac cagtatcatg ctctgttcag ccatgcttct
    54721 gccgttcagg tattaggggc gaaagatgat ggcagcgata agcgcctcgg tgatggacaa
    54781 accgcccgcc cggtgtggga aaccgccaaa gatgcgctgc aatttggtca ggacggtcac
    54841 ggtagcggtt tcttctttac tgaaaaaccg gatgctaatg tctgggtcga tggcgcagtt
    54901 tcaagctatt accgcgaaac ctatgccgaa gcagaacaac gtttaggtga agttcgcgcc
    54961 ctgcgcctgg cgggtcataa caatattttc cccacgcttt catggctcaa cggcactgcc
    55021 acgctccgcg tctggcatcc gcgcggccct gatcaagttg aagtgtgggc gttctgtatt
    55081 actgacaaag ccgcctccga tgaagttaaa gccgcttttg aaaacagcgc cactcgtgct
    55141 tttggtcctg ctggttttct cgagcaggat gactcggaga actggtgtga aatccagaaa
    55201 ttgcttaaag gccaccgcgc ccgcaacagc aaactgtgtc tggaaatggg gcttggtcag
    55261 gaaaagcgcc gcgacgacgg cattcctggc attactaact atatcttttc agaaactgcc
    55321 gctcgtggaa tgtaccaacg ctgggccgat cttctgagta gcgaaagctg gcaggaagtg
    55381 ctcgataaaa ccgccgctta ccagcaggag gtgatgaaat gagtgcgcaa gtttcactag
    55441 agttacatca ccgcattagc cagtttctct ttcacgaagc cagcttactg gacgactgga
    55501 aatttcgtga ctggctggag cagctcgaca aagagattcg ttacaccatg cgcaccacag
    55561 ttaacgcgca aacacgcgac cgccgcaaag gcgtccagcc accgacaacc tggattttta
    55621 atgacaccaa agaccagctg gagcggcgaa tcgcccgtct ggaaacgggc atggcctggg
    55681 cagaagagcc gccgtcacgc acccgtcact taatcagcaa ctgccagata agcgaaaccg
    55741 acatcccaaa cgtatttgct gtgcgggtaa attatctgct ttatcgggca caaaaagagc
    55801 gcgatgaaac attctatgtt ggaacgcgtt tcgacaaagt tcgccgtctg gaagatgaca
    55861 actggcgctt gctggaacgg gatatcgtcc tggatcaagc ggtaatcact tcccataacc
    55921 tgagtgtact gttctgatga atcgaattta tgcgtgtccc gttgcggatg tgccggaggg
    55981 tgaggctctc cggatcgata cctcgcccgt catcgccctg ttcaacgttg gcggcgagtt
    56041 ttatgccatt aacgatcgtt gcagccatgg taatgcgtcg atgtcagaag ggtatctgga
    56101 agatgacgcc acggtggagt gcccgctaca cgccgccagt ttttgcctga aaacggggaa
    56161 agcgttatgc ctgcccgcca ccgatccgct caccacttat ccagtacacg ttgaaggtgg
    56221 tgacattttc atcgacttac cggaggcgca gccatgagcg atctgcataa cgagtccatt
    56281 tttattaccg gcggcggatc gggattaggg ctggcgctgg tcgagcgatt tatcgaagaa
    56341 ggcgcgcaag ttgccacgct ggaactgtcg gcggcaaaag tcgccagtct gcgtcagcga
    56401 tttggcgaac atattctggc ggtggaaggt aacgtgacct gttatgccga ttatcaacgc
    56461 gcggtcgatc agatcctgac tcgttccggc aagctggatt gttttatcgg caatgcaggc
    56521 atctgggatc acaatgcctc actggttaat actcccgcag agacgctcga aaccggcttc
    56581 cacgagctgt ttaacgtcaa cgtactcggt tacctgctgg gcgcaaaagc ctgcgctccg
    56641 gcgttaatcg ccagtgaagg cagcatgatt ttcacactgt caaatgccgc caggtatcct
    56701 ggcggcggtg gcccgctgta caccgccagt aaacatgccg caaccggact tattcgccaa
    56761 ctggcttatg aactggcacc gaaagtgcgg gtgaatggcg tcggcccgtg tggtatggcc
    56821 agcgacctgc gcggcccaca ggcgctcggg caaagtgaaa cctcgataat gcagtctctg
    56881 acgccggaga aaattgccgc cattttaccg ctgcaatttt tcccgcaacc ggcggatttt
    56941 acggggccgt atgtgatgtt ggcatcgcgg cgcaataatc gcgcattaag cggtgtgatg
    57001 atcaacgctg atgcgggttt agcgattcgc ggcattcgcc acgtagcggc tgggctggat
    57061 ctttaaggaa gcacgatgaa agaaaaaacg atcattattg tcggtggcgg gcaagcggcg
    57121 gcaatggctg cggcctcgct acgccagcaa gggttcaccg gtgagctgca tctgttttcc
    57181 gatgagcgac atcttcctta tgaacgacct ccactctcga aatccatgtt gctggaagat
    57241 tccccgcagt tacagcaggt gttacccgct aactggtggc aggaaaacaa tgttcatctg
    57301 cattccggtg taaccatcaa aacgctgggt cgcgacacac gagagttagt gttaaccaac
    57361 ggcgaaagct ggcactggga tcagcttttt atagcaatcg gcgcggcagc tcgaccgctg
    57421 ccgttgcttg atgcactggg agaacgctgc tttaccctac gccatgccgg tgatgccgcc
    57481 agactgcgag aagttctgca gcccgaacgg tcagtcgtga ttgtcggtgc cggaactatt
    57541 ggtctggaac tggctgccag cgccacgcag cgcagatgta aggtgacagt gattgaactg
    57601 gcggcaaccg tcatgggccg taatgcacca ccgcccgtgc aacgctatct tttacagcgc
    57661 caccagcagg ctggtgtgcg cattctgctc aataatgcca ttgaacatgt ggtcgatggt
    57721 gaaaaagtag aactgacgct gcaaagtggg gaaacgcttc aggctgatgt ggtgatttac
    57781 ggtattggta tcagcgccaa cgagcaactg gctcgcgagg ccaaccttga tactgccaat
    57841 ggcattgtca ttgatgaggc ttgccgcacc tgcgatcccg cgatctttgc cggtggcgat
    57901 gtggcaatca ctcgtcttga taatggtgca ctacaccgct gcgaaagctg ggaaaacgcc
    57961 aataaccagg cgcaaattgc cgctgccgca atgttagggc taccgctacc gctactgccg
    58021 ccgccgtggt tctggagcga tcagtacagt gataacttac agtttattgg cgatatgcgt
    58081 ggcgatgact ggctttgtcg tggcaacccg gaaactcaga aggcgatttg gtttaatctg
    58141 caaaacggcg tgcttatcgg tgcggtcacg ctgaatcagg ggcgtgagat tcgcccaatt
    58201 cgcaaatgga tccagagcgg caaaacgttt gatgcgaaac tgctgataga tgagaacatc
    58261 gcgcttaaat cactgtaacc aggataatta gcgaatatct caacgcctgg ggcgtggcga
    58321 ggtgcaagag tgtgtattac gtttaaatca cattatcttg caaagggatt ggttatgaac
    58381 acactacgt
//
LOCUS       DAESHI010000013        57430 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.013, whole genome shotgun sequence.
ACCESSION   DAESHI010000013 DAESHI010000000
VERSION     DAESHI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.013"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            146..1489
                     /gene="argG"
                     /locus_tag="KW011_000816"
     CDS             146..1489
                     /gene="argG"
                     /locus_tag="KW011_000816"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBH7659234.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1497..3104)
                     /locus_tag="KW011_000817"
     CDS             complement(1497..3104)
                     /locus_tag="KW011_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBH7659235.1"
                     /translation="MLEDFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFFVLTILV
                     VKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDPDEVVKM
                     LGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQFAYKDA
                     KNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVRDTGIDT
                     YVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQSISGAPYTALSVPLSLTAD
                     SVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETVYVRGFD
                     ELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDACYDNSIH
                     YTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHVPMFIWY
                     SPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDSRVVDAN
                     HDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(3582..3668)
                     /locus_tag="KW011_000818"
     tRNA            complement(3582..3668)
                     /locus_tag="KW011_000818"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3632..3634),aa:Leu,seq:gag)
     gene            complement(3683..4015)
                     /gene="secG"
                     /locus_tag="KW011_000819"
     CDS             complement(3683..4015)
                     /gene="secG"
                     /locus_tag="KW011_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBH7659236.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4243..5580)
                     /gene="glmM"
                     /locus_tag="KW011_000820"
     CDS             complement(4243..5580)
                     /gene="glmM"
                     /locus_tag="KW011_000820"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBH7659237.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5573..6421)
                     /gene="folP"
                     /locus_tag="KW011_000821"
     CDS             complement(5573..6421)
                     /gene="folP"
                     /locus_tag="KW011_000821"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBH7659238.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6511..8445)
                     /gene="ftsH"
                     /locus_tag="KW011_000822"
     CDS             complement(6511..8445)
                     /gene="ftsH"
                     /locus_tag="KW011_000822"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBH7659239.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8545..9174)
                     /gene="rlmE"
                     /locus_tag="KW011_000823"
     CDS             complement(8545..9174)
                     /gene="rlmE"
                     /locus_tag="KW011_000823"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HBH7659240.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9300..9593
                     /gene="yhbY"
                     /locus_tag="KW011_000824"
     CDS             9300..9593
                     /gene="yhbY"
                     /locus_tag="KW011_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBH7659241.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9749..10225)
                     /gene="greA"
                     /locus_tag="KW011_000825"
     CDS             complement(9749..10225)
                     /gene="greA"
                     /locus_tag="KW011_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBH7659242.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10473..11906
                     /gene="dacB"
                     /locus_tag="KW011_000826"
     CDS             10473..11906
                     /gene="dacB"
                     /locus_tag="KW011_000826"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBH7659243.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(12092..13264)
                     /gene="cgtA"
                     /locus_tag="KW011_000827"
     CDS             complement(12092..13264)
                     /gene="cgtA"
                     /locus_tag="KW011_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HBH7659244.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13280..14245)
                     /locus_tag="KW011_000828"
     CDS             complement(13280..14245)
                     /locus_tag="KW011_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBH7659245.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14372..14629)
                     /gene="rpmA"
                     /locus_tag="KW011_000829"
     CDS             complement(14372..14629)
                     /gene="rpmA"
                     /locus_tag="KW011_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBH7659246.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14650..14961)
                     /gene="rplU"
                     /locus_tag="KW011_000830"
     CDS             complement(14650..14961)
                     /gene="rplU"
                     /locus_tag="KW011_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBH7659247.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15220..16191
                     /gene="ispB"
                     /locus_tag="KW011_000831"
     CDS             15220..16191
                     /gene="ispB"
                     /locus_tag="KW011_000831"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HBH7659248.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16419..16697
                     /gene="sfsB"
                     /locus_tag="KW011_000832"
     CDS             16419..16697
                     /gene="sfsB"
                     /locus_tag="KW011_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HBH7659249.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16745..18004)
                     /gene="murA"
                     /locus_tag="KW011_000833"
     CDS             complement(16745..18004)
                     /gene="murA"
                     /locus_tag="KW011_000833"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBH7659250.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18059..18313)
                     /gene="ibaG"
                     /locus_tag="KW011_000834"
     CDS             complement(18059..18313)
                     /gene="ibaG"
                     /locus_tag="KW011_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HBH7659251.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18473..18766)
                     /gene="mlaB"
                     /locus_tag="KW011_000835"
     CDS             complement(18473..18766)
                     /gene="mlaB"
                     /locus_tag="KW011_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HBH7659252.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18766..19401)
                     /gene="mlaC"
                     /locus_tag="KW011_000836"
     CDS             complement(18766..19401)
                     /gene="mlaC"
                     /locus_tag="KW011_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HBH7659253.1"
                     /translation="MFKRLMMVALLVIAPLSAATEADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19420..19971)
                     /gene="mlaD"
                     /locus_tag="KW011_000837"
     CDS             complement(19420..19971)
                     /gene="mlaD"
                     /locus_tag="KW011_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HBH7659254.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(19976..20758)
                     /gene="mlaE"
                     /locus_tag="KW011_000838"
     CDS             complement(19976..20758)
                     /gene="mlaE"
                     /locus_tag="KW011_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HBH7659255.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20766..21575)
                     /gene="mlaF"
                     /locus_tag="KW011_000839"
     CDS             complement(20766..21575)
                     /gene="mlaF"
                     /locus_tag="KW011_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HBH7659256.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21785..22762
                     /locus_tag="KW011_000840"
     CDS             21785..22762
                     /locus_tag="KW011_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBH7659257.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22776..23762
                     /gene="kdsD"
                     /locus_tag="KW011_000841"
     CDS             22776..23762
                     /gene="kdsD"
                     /locus_tag="KW011_000841"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HBH7659258.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23783..24349
                     /gene="kdsC"
                     /locus_tag="KW011_000842"
     CDS             23783..24349
                     /gene="kdsC"
                     /locus_tag="KW011_000842"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HBH7659259.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24346..24921
                     /gene="lptC"
                     /locus_tag="KW011_000843"
     CDS             24346..24921
                     /gene="lptC"
                     /locus_tag="KW011_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HBH7659260.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24890..25447
                     /gene="lptA"
                     /locus_tag="KW011_000844"
     CDS             24890..25447
                     /gene="lptA"
                     /locus_tag="KW011_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HBH7659261.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25454..26179
                     /gene="lptB"
                     /locus_tag="KW011_000845"
     CDS             25454..26179
                     /gene="lptB"
                     /locus_tag="KW011_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HBH7659262.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26227..27660
                     /gene="rpoN"
                     /locus_tag="KW011_000846"
     CDS             26227..27660
                     /gene="rpoN"
                     /locus_tag="KW011_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBH7659263.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27683..27970
                     /gene="hpf"
                     /locus_tag="KW011_000847"
     CDS             27683..27970
                     /gene="hpf"
                     /locus_tag="KW011_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HBH7659264.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28088..28579
                     /gene="ptsN"
                     /locus_tag="KW011_000848"
     CDS             28088..28579
                     /gene="ptsN"
                     /locus_tag="KW011_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HBH7659265.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28625..29479
                     /gene="rapZ"
                     /locus_tag="KW011_000849"
     CDS             28625..29479
                     /gene="rapZ"
                     /locus_tag="KW011_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBH7659266.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29476..29748
                     /gene="npr"
                     /locus_tag="KW011_000850"
     CDS             29476..29748
                     /gene="npr"
                     /locus_tag="KW011_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HBH7659267.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            29962..30594
                     /gene="yrbL"
                     /locus_tag="KW011_000851"
     CDS             29962..30594
                     /gene="yrbL"
                     /locus_tag="KW011_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HBH7659268.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30591..31319)
                     /gene="mtgA"
                     /locus_tag="KW011_000852"
     CDS             complement(30591..31319)
                     /gene="mtgA"
                     /locus_tag="KW011_000852"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HBH7659269.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31316..31969)
                     /gene="elbB"
                     /locus_tag="KW011_000853"
     CDS             complement(31316..31969)
                     /gene="elbB"
                     /locus_tag="KW011_000853"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HBH7659270.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32199..34535)
                     /gene="arcB"
                     /locus_tag="KW011_000854"
     CDS             complement(32199..34535)
                     /gene="arcB"
                     /locus_tag="KW011_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HBH7659271.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34631..35560)
                     /locus_tag="KW011_000855"
     CDS             complement(34631..35560)
                     /locus_tag="KW011_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBH7659272.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36235..40695
                     /gene="gltB"
                     /locus_tag="KW011_000856"
     CDS             36235..40695
                     /gene="gltB"
                     /locus_tag="KW011_000856"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HBH7659273.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40708..42126
                     /gene="gltD"
                     /locus_tag="KW011_000857"
     CDS             40708..42126
                     /gene="gltD"
                     /locus_tag="KW011_000857"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HBH7659274.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42310..42789
                     /locus_tag="KW011_000858"
     CDS             42310..42789
                     /locus_tag="KW011_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HBH7659275.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            42806..43111
                     /locus_tag="KW011_000859"
     CDS             42806..43111
                     /locus_tag="KW011_000859"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH7659276.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(43187..43651)
                     /locus_tag="KW011_000860"
     CDS             complement(43187..43651)
                     /locus_tag="KW011_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBH7659277.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(43648..44523)
                     /gene="nanK"
                     /locus_tag="KW011_000861"
     CDS             complement(43648..44523)
                     /gene="nanK"
                     /locus_tag="KW011_000861"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HBH7659278.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(44520..45209)
                     /locus_tag="KW011_000862"
     CDS             complement(44520..45209)
                     /locus_tag="KW011_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBH7659279.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(45257..46747)
                     /gene="nanT"
                     /locus_tag="KW011_000863"
     CDS             complement(45257..46747)
                     /gene="nanT"
                     /locus_tag="KW011_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HBH7659280.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(46857..47750)
                     /gene="nanA"
                     /locus_tag="KW011_000864"
     CDS             complement(46857..47750)
                     /gene="nanA"
                     /locus_tag="KW011_000864"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBH7659281.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(47872..48663)
                     /gene="nanR"
                     /locus_tag="KW011_000865"
     CDS             complement(47872..48663)
                     /gene="nanR"
                     /locus_tag="KW011_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HBH7659282.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            49043..50365
                     /gene="dcuC"
                     /locus_tag="KW011_000866"
     CDS             49043..50365
                     /gene="dcuC"
                     /locus_tag="KW011_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBH7659283.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(50408..50905)
                     /gene="sspB"
                     /locus_tag="KW011_000867"
     CDS             complement(50408..50905)
                     /gene="sspB"
                     /locus_tag="KW011_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HBH7659284.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(50911..51549)
                     /gene="sspA"
                     /locus_tag="KW011_000868"
     CDS             complement(50911..51549)
                     /gene="sspA"
                     /locus_tag="KW011_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HBH7659285.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(51944..52336)
                     /gene="rpsI"
                     /locus_tag="KW011_000869"
     CDS             complement(51944..52336)
                     /gene="rpsI"
                     /locus_tag="KW011_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBH7659286.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(52352..52780)
                     /gene="rplM"
                     /locus_tag="KW011_000870"
     CDS             complement(52352..52780)
                     /gene="rplM"
                     /locus_tag="KW011_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBH7659287.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(52999..54126)
                     /gene="zapE"
                     /locus_tag="KW011_000871"
     CDS             complement(52999..54126)
                     /gene="zapE"
                     /locus_tag="KW011_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HBH7659288.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            54320..54718
                     /locus_tag="KW011_000872"
     CDS             54320..54718
                     /locus_tag="KW011_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HBH7659289.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            54872..56239
                     /gene="degQ"
                     /locus_tag="KW011_000873"
     CDS             54872..56239
                     /gene="degQ"
                     /locus_tag="KW011_000873"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HBH7659290.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            56329..57396
                     /gene="degS"
                     /locus_tag="KW011_000874"
     CDS             56329..57396
                     /gene="degS"
                     /locus_tag="KW011_000874"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HBH7659291.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT        14258 a        15305 c        14260 g        13607 t
ORIGIN      
        1 agcgcattat tttacaaaaa acacactaaa ctcttcctgt ctccgataaa agatgattaa
       61 atgaaaactc atttattttg cataaaaatt cagtgagagc ggaaatccag gctcatcatc
      121 agttaattaa gcagggtgtt attttatgac gacgattctc aagcatctcc cggtaggtca
      181 acgtattggt atcgcttttt ccggcggtct ggacaccagt gccgcactgc tgtggatgcg
      241 acaaaaggga gcggttcctt atgcatatac tgcaaacctg ggccagccag acgaagagga
      301 ttatgatgcg atccctcgtc gtgccatgga atacggcgcg gagaacgcac gtctgatcga
      361 ctgccgcaaa caactggtgg ccgaaggtat tgccgctatt cagtgtggcg catttcataa
      421 caccaccggc ggcctgacct atttcaacac gacgccgctg ggccgcgccg tgactggtac
      481 catgctggtt gctgcgatga aagaagatgg cgtgaatatc tggggtgacg gtagcaccta
      541 taaaggaaac gatatcgaac gtttctatcg ttatggtctg ctgaccaatg ctgaactgca
      601 gatttacaaa ccgtggcttg atactgactt tattgatgaa ctgggcggcc gtcatgagat
      661 gtctgaattt atgattgcct gcggtttcga ctacaaaatg tctgtcgaaa aagcctactc
      721 cacagactcc aacatgcttg gtgcaacgca cgaagcgaag gatctggaat acctcaactc
      781 cagcgtcaaa atcgtcaacc caattatggg cgtgaaattc tgggatgaga gcgtgaagat
      841 cccggcagaa gaagtcacag tacgctttga gcaaggtcat ccggtagcgc tgaacggtaa
      901 aacctttagc gacgacgtag aaatgatgct ggaagctaac cgcatcggcg gtcgtcacgg
      961 cctgggcatg agcgaccaga ttgaaaaccg tatcatcgaa gcgaaaagcc gtggtattta
     1021 cgaagctccg gggatggcac tgctacatat cgcttatgaa cgtctgttga ccggtattca
     1081 caacgaagac accattgagc agtatcatgc acatggtcgt cagttgggcc gtctgctgta
     1141 ccaggggcgt tggtttgatt cccaggcgct gatgctgcgt gactctctgc aacgctgggt
     1201 tgccagccag atcactggtg aagttaccct ggagctgcgc cgtgggaacg attattcaat
     1261 cctgaatacc gtctcagaga acctgaccta caagccagag cgtctgacga tggaaaaagg
     1321 cgactcggtg ttctcgccag atgatcgtat tggtcaattg accatgcgta acctggatat
     1381 cactgatacc cgagagaaac tttttggtta tgccaaaact ggcctgctct cctcctctgc
     1441 cacttcaggc gtgccgcagg tggagaatct ggaaaacaaa ggccagtaat tcgacatcaa
     1501 ccctgccctt caggggtggg gttaccttgc ttatcctctg taaactcctt tctgagcatc
     1561 acataatcga aaacatcatg gtttgcatcc acaacccgtg agtctccttt atagtgtgca
     1621 atcacttcct ccagcgtttt cggttgttcc ggctttgtta cccccatcca cgcattaata
     1681 aggtaattat tgtaagctgt cgaaaagatg ttgttttccg ttgtacgatc cacgccatcc
     1741 ccaagaacgg ggctatacca gataaacatc gggacatgat atgcctgctg gctagcctcc
     1801 ctgcctccat gaaaatagac gttcttcttc gtagggtcac gttccaggcc gtggtcggca
     1861 aaatacatga ccgaggcgcg gcgatctttt aataattcaa aaacctgacc tagcaaacta
     1921 tctgtgtaat gaatggagtt gtcatagcag gcatcctgat cgtcctgcgg ttgaaacacg
     1981 gcgctggatt gcggataggc gctacaagcc ggttcatggc ttccatttaa atgaagaaca
     2041 atcagtttct tttgctgcgt attttgctgt aatgcttgcg ataaatgcgg caacaacaat
     2101 tcatcaaatc ctctgacata aactgtttcc atggcgcgca tggcgatact ggtaactgct
     2161 gtaccattct gccgaaaagc ggattgcgag cttagccaga aagtctgaaa tcctgcttga
     2221 ttagccatat taataatgtt gtcggggtaa ttatgaatgt catgactcaa aacagaatca
     2281 gcagttaaag aaaggggaac cgacagcgca gtgtaaggtg cgccgcttat tgattgatta
     2341 aacagtttga tctgttttct ttgtgcttca acttgcggtg tcgtagagcg tgtatatcca
     2401 tacaaagaca tattgtcgac acgtacagac tcccccacaa tcaacacgta ggtatcaata
     2461 cctgtatccc tgactgataa ttgaaaatac ggcaccgtgt ttgcaattga gagtaatctt
     2521 tgatgctctt tcgctgctaa agcaaaataa ttgagattga aaaacggcgt ataggtagca
     2581 aatcgcgacg ctagtatata tggactgaat gcttttttat tttttgcatc tttataagca
     2641 aattgacaag cagaaaataa actgcccgaa atgacaatca gcaataatat tcctgtcact
     2701 ttttttgtcg gcaaggaaac atcatatttt attattactg ccaaaaaaag aagggataaa
     2761 aaagcaaaag cacatagata agggatatac atccccagca ttttgacaac ttcatccgga
     2821 tcactctgca gcacactaat cgcaaatcca tcattgaaag ttgtaccaaa ggtacaccat
     2881 gaatagaggc taatactcat gtctgccgca gtcagtaaca caaatggtgc ggcaacaagc
     2941 aggcgaagcg gaagggatga tatacgtttt acaaccagga tggtcaggac gaagaacagc
     3001 aatcgctgaa ccatatgcgc gccggaagag gcgactaagt tcgtaatacc aaaaacaata
     3061 acacaaagat acaacaacca atgagattta aaatcttcta gcaaatttgt tgaatactgt
     3121 catccagaaa tccaagttca caaccaatat aaacaattat caacaatgtt caattttata
     3181 tcagacatgt ttttcaactc tgatccatat cagaccgaaa cactgttagt tttttctttt
     3241 taaatgatgc ggccattgcg ttctcagtaa ttaaataaca ttatcaatgc gtctctaaac
     3301 attacgcaga acgtagccat cagtagttat actgacagga gcaggatggt ttatcgagaa
     3361 gtaaacatat ccgtattcgt accggaactg gagttgacac aataaagtgc caattatgtc
     3421 agcagaaggg aaaagtttat aactaaggcg taccggcacc atcgtttcaa ggtaccagct
     3481 acgagtaaag caactggacg agatacagat acctgacaac cattcctcag accaggacca
     3541 aaacgaaaaa agacgctttt cagcgtctct tttctggaat ttggtaccga ggacgggact
     3601 tgaacccgta agccctattg ggcactacca cctcaaggta gcgtgtctac caattccacc
     3661 acctcggcac ggatactact ttttagttcg ggatatcgct ggtcggctta gccggagcag
     3721 ctggctgagt ttgttcggtt ttcgccggtg cactcagatt ttcccattcg ctacctttat
     3781 tggttttgtt gctattgatg ttacccagca ccagactgat gatgaagaat aacgttgcca
     3841 gcagcgccgt catgcgggtc atgaagttac cagaaccact tgaaccaaac agcgtagcgg
     3901 aagcgcctgc tccgaaggag gctcccatat cagcgccttt accttgctgc agcatgatca
     3961 gaccaacaag gccaattgcc acaataagga aaactaccaa aagagcttcg tacataatca
     4021 acctgttcct tgcggagttg ccgcgtacca atgcttcaac caataaagcg ggagttttta
     4081 atctttccca ctgaagtggg tgtgaatact aaccaaagcg aatgaccttc gcaagggcaa
     4141 ttttatcgca ttgtatcaac tgcggaaaaa aacagcaaaa ccgatgtgtt cgctgagaaa
     4201 aaaggcgaca atcgccgcct ttttagccag ttatttaccg ttttaaacgg cttttactgc
     4261 atcggcgata cggtgtgcaa attcagttac ctgcgcttcg tcttcacctt ctaccatcac
     4321 gcgaattaac ggttcggtgc cggatttacg caacagcaca cgtccacggc tacccagcgc
     4381 cgcttcaact tcggcggtca cagctttaac tgactcatgc tcaagcggat cgccgctacc
     4441 tgcggtgtaa cgcacgttaa ccagaatctg cgggaacatt ttcatgccgc tgcaaaggtc
     4501 gtgcaggctc atatggttac gtgccatcgc cgccagcacc tgcaagccag caacgatgcc
     4561 gtcaccggta gtggttttat ccagcaggat cacatgaccg gaattctctg caccgatacg
     4621 ccagcctttc tcctgcattt tttccagtac gtagcggtca cccactttcg cgcgcgcaaa
     4681 tggaattcct aactgtttca acgccagctc aagccccatg ttactcatca atgtacccac
     4741 agcaccacca cgcagctggc cctgacgaag accttcacgc gcgatgatat acatgatctg
     4801 atcaccatcg actttattgc cttcatggtc aaccataatc acgcgatcgc cgtcgccgtc
     4861 gaaggcaata ccgagatccg ctttttcagc cagcacacga gcctggagcg cgcgaacgtc
     4921 ggtagccccc acttcggcat tgatgtttac accgttcggc tcacaaccga tagcgataac
     4981 gttcgccccc agttcacgca gcacgttcgg cgcgatgtga taagtcgcac cgtttgcgca
     5041 atccaccaca atcttcagtt cgctgaggct aagttcgttc gggaacgtgg ctttgcaaaa
     5101 ctcgatatag cgacccgcag catcaacgat acggctggct ttacccagtt ctgccgaatc
     5161 aacacagctg atctcttttt ccatttccgc ttcgatggcc tcttctaccg catccggtag
     5221 tttggtgccg tcgatagaga aaaatttaat gccattatcg tagaacgggt tatgcgatgc
     5281 agatatcaca attccggcct ctgcgcggaa ggtacgcgtc agataagcca cggccggtgt
     5341 tggcatcggg ccagtgaaga gtgcggaaag gcccgctgcc gccagacccg cttccagtgc
     5401 tgactccagc atatagccag aaatacgcgt gtctttacca ataatgatct tacgggagcc
     5461 gtggcgcgcc agcactttac ccgcggccca acccagctta agcacaaaat caggtgtgat
     5521 cggcgcatcc cctacacgac cacgaatccc atcggtaccg aaatatttac gattactcat
     5581 agcgtttgtt ttcctttgca gacagagtgg cttccaccac ccgcatcgct tctacggttt
     5641 ctttgacgtc atgaacacga ataatgtgcg cgccttgcat tgcggcaatg accgcacagg
     5701 ccagactacc gctcaggcgc tcggacggcc ccacgttcag cagctgccca atcatcgatt
     5761 ttcgtgacat acctaccaac agcggcaggt tgaaatggtg aaattcagcc aggcgcgcca
     5821 gtaatgaata gttatgggag agatttttac cgaaaccgaa tccggggtcg agcaacaatt
     5881 tctcttttgc gatacccgcc tgctcgcaac gtgctatttg ctcaataaag tagcgattca
     5941 cttctgcaaa gacatcgtca tacttcggag cttcctgcat ggtttttgga tttccctgca
     6001 tatgcatcag acaaaccggt aaaccggttt ctgcagccgc ctccagagcg ccaggttcgg
     6061 aaagggagcg gatatcgtta ataatgtgag cgccaacttt cgctgactca cggatgactt
     6121 ctggtttgga tgtatctacc gagatccaga cttcgaagcg ttgagcaatt gcctcaacca
     6181 caggaataac acgttgcaac tcttcttcaa cgctaacttc cgccgcccct gggcgcgtgg
     6241 actcgccacc aacgtcaatg atcgtcgcgc cagcgttgat catcagattc gcatgtttca
     6301 ccgcatctat cagcgagtta tgcgtgccac catccgaaaa ggaatcaggc gtgacgttga
     6361 ggatccccat tacgtgagga tggctaaggt ccagtgaagt gccctgggca aagagtttca
     6421 tggtgttatc cctggtatga attgataaga aaaaaccccg gagcacgccc cggggttttc
     6481 ggtacaaata cagtcatctg atgcgggaac ttacttgtcg cctaactgct ctgacatggt
     6541 gttacccggg ttcggcgtac gcggttcatc aaccggacga ggagcctttg gactaccatt
     6601 gtcgccagaa ttgttagaag cgcctggttc ttcccagccc gctggcggac gtacatcgcg
     6661 acgtgccatc aggtcatcaa tctgcggtgc gtcgatagtc tcatatttca tgagagcatc
     6721 tttcatcgca tgcagaatat ccatattgtc ggtcagaagc tgacgcgcac gattatagtt
     6781 acgctcaatc agtgctttca cttcctggtc gatgatacgt gcagtttcat cggacatatg
     6841 tttcgctttc gctacgctac ggccgaggaa cacttcacct tcttcttccg cgtacagcag
     6901 tggacccaat ttctcagaga agccccactg agtcaccatg ttacgcgcca ggttggtcgc
     6961 aactttaata tcgttggacg caccggtaga aacatgttcc ggcccgtaga tgatctcttc
     7021 tgccagacga ccaccgtaca gcgtagaaat ctggctttcc agtttctgac ggctggcgct
     7081 gattgcgtcg ccctcaggca agaagaaagt cacacccagc gcacgaccgc gtgggataat
     7141 cgtcactttg tgcaccggat cgtgttccgg caccaggcga ccgataatcg catgacccgc
     7201 ttcgtggtaa gccgtcgatt ctttctgcgc ttccgtcatc accatggagc gacgttccgc
     7261 acccatcatg attttatctt tcgctttctc gaactcaacc atcgacacaa cgcgtttgtt
     7321 gccacgagca gcgaacagtg ccgcttcgtt taccaggttc gccaggtcag caccggagaa
     7381 accaggagta ccacgggcaa tgattgccgc gtcgatatcg ggtgccaatg gtacgcgacg
     7441 catgtgaact ttcaggatct gctcacgacc gcgaacatct ggcaagccaa ccacaacctg
     7501 acggtcgaaa cggccaggac gcagcagggc cgggtcaaga acgtccggac ggttagtcgc
     7561 ggcgataacg atgatacctt cgttaccttc gaagccatcc atctcaacca gcatctggtt
     7621 cagagtctgt tcacgttcat cgtgaccacc gcccagacca gcgccacgct ggcggcctac
     7681 ggcgtcgatt tcatcgataa agatgatgca cggtgccgct ttcttcgcct gttcgaacat
     7741 gtcacgaaca cgggatgcac ccacaccgac gaacatttct acgaagtcag aaccggagat
     7801 agtaaagaac ggaactttcg cttcgcctgc aatcgctttc gccagcagcg ttttaccggt
     7861 acccggagga ccgaccatca ggacgccttt cgggatctta ccgccgagtt tctggaagcg
     7921 gctcggctcg cgcagatatt caaccagttc agcaacttct tcttttgctt cgtcgcagcc
     7981 cgcaacgtca gcaaaggtcg ttttgatctg atcttccgtc agcatgcgcg ctttgctctt
     8041 accaaacgac atggcacctt tgccaccgcc gccctgcatt tgacgcatga agaagatcca
     8101 cacaccaatc agcaacagca tcgggaacca ggagatgaag atagaagcca gcaggcttgg
     8161 ttcttcaggc ggttcaccga caaccttgac gttcttggtc aacaggttat ccagtaattt
     8221 cggatcctga accggaatgt aagtggtata acggttacta tctttcttgg taacgttgat
     8281 ttcacgtccg ttgatacgcg cttcacgaac ctggtcgtta ttgacctctt gtaggaaggt
     8341 agagtaatcc accttacggc cattagactc gctgggccca aagctctgga atactgacat
     8401 cagcacaacg gcaatgacca gccagagtat taggtttttc gccatgtcac tcaagggatt
     8461 aacctcttat tacaactgtg ttaaaaacag cgtcaggata ctctatatcc agcatctttc
     8521 aaactttcgt ctgaaatctc ccggttaggg tttacgcccg gtcgctacaa tatacacttc
     8581 ccgcgaacgt gcacgagaag agtccggctt acgaactttg accttcgtaa acagggagcg
     8641 aatttccctt agatactcat cgaaaccttc gccctggaac accttcacta caaaactgcc
     8701 acctggcgct aatacatcac gacacatttc tagcgccagt tccaccagat acatggcacg
     8761 ggggatatcc accgccggtg ttccgctcat gtttggtgcc atatcggaca tgacaacctg
     8821 gactttgctg tcgccaacgc gctccagcag tgctttcatc accagttcat cacgaaaatc
     8881 gccctgaaga aagtccacac caacgatagg atccataggt aaaagatcgc aagcgatgat
     8941 gcggcctttg ccgccaattt gggtgaccac atattgtgac caaccacccg gagcagcacc
     9001 aaggtcgaca accgtcattc ccggtttaaa gagtttgtca ctttgctgta tttcatcaag
     9061 tttaaaccag gcacgggaac gtaacccctt tttctgtgcc tgttgaacat atttatcgct
     9121 aaagtgttcc tgaagccagc ggctggagct ggcagaacgc ttcttacctg tcatttaact
     9181 ttcccatggg gataactcat cgtaaccaat tgcgtaaatt tttacgcgcc tatttggtga
     9241 tatatgggag atggcggtag aatgacccat tttcaatccc aacgtaagca aaaaatacga
     9301 tgaatctgag tactaaacaa aaacagcacc tgaaaggtct ggcacatccg ctcaagccag
     9361 ttgttctgct tggcagtaat ggtttgaccg aaggggtgct ggccgagatt gaacaagcgt
     9421 tagagcacca tgaactcatc aaggtgaaaa tcgccaccga agatcgcgaa acaaaaacct
     9481 tgatcgtgga agctatcgtg cgcgaaaccg gcgcctgtaa tgtacaggtc atcggtaaaa
     9541 cgctggtgct ttatcgccca actaaagaac gtaaaatctc gctgccacgc taagattatc
     9601 ctaaagttac acacatttgc tgtgtaaaac gaggggtttt ccgcaggcag gagagcaaaa
     9661 tgccacgctc tgttcgttga taaaaggccg catagcggcc tttttccttt ctttacaata
     9721 catcaacatc ttgagtattg ggtaattctt acaggtattc caccttaatt acttcaaatt
     9781 ctacttcgcc gcccggcgtt ttgatgacca caacatcatc ttcttctttg ccgatcaggc
     9841 cacgagcaat aggcgagttt acagaaatca ggttttgttt aaagtcagct tcgtcatcgc
     9901 caacgatgcg ataagtctgt tcttcgtcag aatccagatt cagcaccgtt acggtagcac
     9961 caaaaataac gcgcccattg ttaggcattt tggtgacatc aatcacctgc gcgtttgaca
    10021 gcttggcttc gatgtctttg atacggcctt cgcagaagcc ctgctgttca cgagctgcgt
    10081 ggtattcggc gttttctttc aggtcgccat gctcacgcgc ttccgcgata gcagcaatga
    10141 tttcaggacg gcgcacagat ttcagaaaat ccagctcttc gcgtaatttt tcagcgccgc
    10201 gtaaggtcat cggaatagct tgcatttgtt atacctcttg aatattcctg atggggcaag
    10261 tcttcaccca ccccggctgt tcggcctgcc cggcataact cctcaccggg accagaagca
    10321 aaaaaatacc gacccaggta caagtcccag gtcagctaca attcacattt tgatagtcat
    10381 tttaccctga agttcccgaa gggtcatcgt ttactttata gggcgttgcg ccgtagtatg
    10441 acggctcgat tccaggttgt tagcgcgaga ttatgcgatt ttccagattt atcatcggat
    10501 tgaccagctg tatagcgttc agtgttcagg ccgcaaatgt tgatgagtac attactcaac
    10561 tccccgctgg tgccaacctt gccctgatgg tgcaaaaagt cggcgcgtcg gcccccgcta
    10621 ttgattacca cagtcagcag atggcgctgc ctgccagtac ccagaaagtg attactgcgc
    10681 tggcggcgtt gattcaactc ggccccgatt ttcgttttac cacgacgctt gaaaccaaag
    10741 gcaatgtgga aaacggcgta cttaagggtg acttagtggc gcgatttggt gccgatccga
    10801 cgttaaaacg tcaggatatt cgcaatatgg tcgcgacttt gaaaaaatct ggcgtcaacc
    10861 aaatcgatgg caatgtgttg atagatacct ccattttcgc cagccacgat aaagcccccg
    10921 gctggccatg gaatgacatg acacaatgct ttagcgctcc gcctgccgcc gccatagttg
    10981 accgcaactg tttctccgtc tcgctctaca gtgcccaaaa acccggtgat atggccttta
    11041 tacgcgtggc atcttattac cccgttacga tgttcagcca ggtacgcacc ctcccccgtg
    11101 gttctgccga agcgcaatac tgcgaactgg atgtcgtacc aggcgacctg aaccgcttta
    11161 cgctgacggg atgcctgcca caacgttctg agccgctccc gttggctttt gccgtgcagg
    11221 atggagccag ttatgccggt gcaattctga aagatgagtt aaaacaggcg ggtatcacct
    11281 ggagcggaac actgctgcgc cagactcagg ttaacgaacc tggaacggta gttgccagta
    11341 aacagtcggc cccgctgcac gatctgctta agattatgct gaaaaagtcg gataacatga
    11401 tcgccgatac ggttttccgc atgataggcc atgcgcgctt caatgtacct ggtacatggc
    11461 gggccgggtc ggacgctgtg cgtcagatcc tgcgccagca agccggtgtc gatattggaa
    11521 acaccattat tgccgatggt tcagggcttt cgcggcataa cctgattgcc cccgccacca
    11581 tgatgcaggt gctgcaatac attgcccaac acgacaatga acttaacttt atctccatgc
    11641 tgccgctggc gggctatgac ggctctttgc agtaccgtgc aggtctgcat caggcgggcg
    11701 tggatggaaa agtctcagcg aaaaccggtt cgttgcaggg ggtatataac ctggcgggat
    11761 tcattaccac agcgagcggg caacgaatgg cgtttgtgca atatctttct ggctatgcag
    11821 tagaacctgc ggatcagcgt aatcgccgta ttccgttagt gcgttttgaa agccgtttgt
    11881 ataaagatat ttatcagaac aattagtcaa aaagaaaccc cggcacatgg ccggggcttc
    11941 agattattga caaagtgcgc tttgtttatg ccggatgcgg cgtaaacgcc ttatccggcc
    12001 tacaaaatcg tgcaaattca acatattgca attctcttgt aggcctgata agcgtagcgc
    12061 atcaggctga tttggcgttt atcatcagtg attaacgctt gtaaatgaac tcaacgcctt
    12121 cttcgtcgtc ttcgtcccag tcgtcatccc agtcttcatc atcctcttca gcaatctctt
    12181 caagctgctg gcgatgataa tcgtcccaca tgaattcgac tttctctggc tgtttcgctt
    12241 cttcagcctg cacgaccggg ttttcaagga taaaggtcat cacatcccag cagagatctt
    12301 tcacgcccag tccgctcgcc gcagagatca gataatattt atcttcccag cccagcgctt
    12361 cagcaatcgc tttcgctttc tcttcagctt ctgccttatc cagcagatcg atcttattga
    12421 acactaacca acgcggtttc gccgccagat cctggctgta tttttccagc tcgctgataa
    12481 taatacgcgc gttttcaacc ggatcggtgc cgtcaatcgg atcgatatcg atgaggtgca
    12541 acaggacgcg gcaacgttcc aggtgcttca ggaagcggat acccagacct gcgccttccg
    12601 cagcgccttc aatcagtcct ggaatatcgg caacaacgaa gctcttttcg ttgtccattc
    12661 gtaccacacc cagacttggc accagagtgg taaacggata atccgccact ttcggtttag
    12721 ccgccgatac cgcgcgaata aaggtcgatt tacccgcgtt tggcatcccc aacataccga
    12781 cgtcagccag cagcatcagc tccagcagca gctcacgctt atcacccggc gtaccgttgg
    12841 ttttctgccg tggtgtacgg ttaacggacg atttgaaacg ggtattgccc agaccgtgcc
    12901 agccgccctt agcaaccagc agacgctgac cgtgtttagt catatcgccc atggtttcac
    12961 cagtaccctg gtcgattaca cgcgtaccta ccggcacttt aatcgtcacg tctttaccgc
    13021 gcttaccggt acaatcgcgg cttgcgccgt tctgaccgcg ctctgcacgg aaagattttt
    13081 caaaacgata atcgataagc gtgttcaggt tttcgtcggc ttccatccat acgtcaccac
    13141 catcaccacc gtcgccgcca tccgggccgc ctttcggaat atacttttcg cggcggaagc
    13201 tcacgcaacc attaccgcca tcacctgcaa cgaccagaat cgatgcttca tcaacaaact
    13261 tcattttatt ctccataaat cattcgcctg cgcggggttg cgataccacc gttgtatgct
    13321 tacgtaatcc gccccaaata cgatgaccaa tggcggaata catcgcgccc gcaaccacga
    13381 caaacgcacc gagataacct aaaaggttta acatcggtct ggcgaagaaa tcgggccagg
    13441 ccagtgataa aagatctgaa aaaaacagcg taaacagtgg ggtgagcgtg atgatcgcgc
    13501 tcacctgcgc tgcctgccag cgagccatcg cttccgccag ggcgccatat cctaccaatg
    13561 tattcagtcc gcaaaaaatt aaacatgcga gctgccagtg gctaagctgc gctatcactc
    13621 caggctttgc cagagggaag agcgcaattg tacataaagt gtacagtaaa aacaggatct
    13681 gcggtgaggc cagccgacgc aataaaacct tttgcgccac gccataactc acccaaaccg
    13741 tcgccgcacc gaccccaaag ataactcccc aggtgtaatc ggtgagcttt gtaaatatct
    13801 cgaccagact ggtgttaaaa aacatcacca ggccgctcag gagcatcaat gcccctacaa
    13861 cctgagtgct gcgcattttc tctttcagga taaatacgct ggcaaccatc atgccaactg
    13921 gcgagagttg cccaatcacc tgcgaagcgg tcggactcag gtattgcaag gatgagctga
    13981 acaggatgaa gttcccaaac agcccggcgg tcgccactgc caacaaaatc aaccagcgtg
    14041 gcttacgaaa cacgcgtaat ggcggcaacc tcttcttcac cgcaagaatg gcaccaaggc
    14101 caatactcgc catcaagaaa cggtaaaaca cgattgtcgg aggttccatc acctccagca
    14161 cctgcttcat tgcgattggc aacgcccccc agcaaattgc tgtggtgagc gccaaaagaa
    14221 tgccaatacc tgcctgctgc ttcataccgt attccctgca aagcgcattt gcggattaac
    14281 gtcgcaccat gcgcattttt accggttatc gaatgtaaaa agccccgcaa cgtgttgcgg
    14341 ggctttcatc cgttaccggg acgcgaaaaa cttattcagc ttcgatgctg ataaatttac
    14401 ggtttttcgg gcctttaact tcgaatttca ctttaccgtc tgctttagca aacagagtgt
    14461 ggtcacgacc gcaacctacg ttagcgccag cgtggaattt ggtaccacgt tgacgaacga
    14521 tgatgctacc cgccagaacg gattcgccac cgaaacgctt aacgcccagg cgtttagctt
    14581 ctgaatcgcg accgttacgt gtggagccgc cagccttttt atgtgccatt tgaaatctct
    14641 cctcaggtct taggcgctga tgccagtaat tttcacatca gtgaaccact gacgatggcc
    14701 ctgctgctta cgatagtgtt tacgacgacg aaacttaacg attttaactt tctcgccacg
    14761 accgtgagca acaacttcag ctttgattac gccgccatca acgaaaggaa cgccgatttt
    14821 gacttcttca ccgtttgcga tcatcagcac ttcagcgaac tcaacagttt cgccagttgc
    14881 gatgtccagc ttttccaggc gaacggtctg accttcgctt actcggtgtt gtttaccacc
    14941 actttggaaa accgcgtaca tataaaactc cgcttccgcg cacacctttt caatgattca
    15001 gagtgcgcta taaatattca caatagggcg cgaatattac gcaaaacgca cgcctttgac
    15061 aagtgctaca gtcaatacac gaagaaaaaa aacacaactt gtacggtaac gtttatctgt
    15121 gccatttttt cagtacaatc accctatatt cctaaccata aaccctaagt tgcctttgtt
    15181 cacagtaagg taatcggggc gaaaagcccg gcttttgcga tgaatttaga aaaaatcaat
    15241 gagttaaccg cgcaagatat ggcgggtgtt aatgcggcaa tccttgagca gcttaattcc
    15301 gacgtccaac tgatcaatca gttaggctat tacatcgtca gcggcggcgg taaacgtatt
    15361 cgtccgatga ttgctgtact ggctgcacga gctgttggct atgagggaaa tgcgcatgtc
    15421 accattgctg ccctgatcga gtttatccac acggcgactc tgctacacga cgacgttgtg
    15481 gatgaatcag atatgcgcag gggtaaagct accgccaacg ccgcatttgg caatgccgcc
    15541 agcgtgctgg taggcgattt tatttatacc cgcgcgttcc agatgatgac cagcctcggt
    15601 tcactcaaag tgctggaagt catgtcagaa gctgtaaacg tcatcgcaga aggtgaagtt
    15661 ctgcaactga tgaacgttaa cgatccggac atcactgaag aaaactacat gcgcgttatc
    15721 tatagcaaaa ccgcgcgtct gtttgaggct gccgcgcagt gttccgggat tctggctggc
    15781 tgtacgccgg aggaggagaa aggcctgcag gattatgggc gctatctcgg cactgctttc
    15841 cagttgatcg acgatttact cgattacaat gccgatggcg aacagttagg taaaaatgtc
    15901 ggcgacgatc tgaacgaagg taaaccgacg ctgccgctgc tgcatgcgat gcatcatggc
    15961 acaccagaac aggcacagat gatccgtacc gccatcgaac agggtaacgg tcgccatctt
    16021 ctggaaccgg ttctggaagc aatgaacgct tgtggatctc ttgaatggac gcgtcagcgt
    16081 gccgaggaag aagcagacaa agccatcgca gcattacagg tgctcccgga caccccttgg
    16141 cgagaagcac tcatcggcct cgcgcacatc gctgttcaac gcgatcgtta atcccctccc
    16201 ttcatcccgc gcagtgcgcg ggatgattcc agtaaattcc ataaaaacac taattcagcg
    16261 ctataacacc tgaaaaaaag ggcgatgaat ctttatatat tctgaatatt cacactcttt
    16321 acaggaactt tttagagcaa taggccatca ggagtatagt gatgcccgac agaagaagtg
    16381 ttctgaatga aagcgactaa cttaaggagt gaggaaagat ggaaagtaat ttcattgact
    16441 ggcatcccgc tgacatcatt gcgggtttgc gcaagaaggg aacgtcaatg gcggcggagt
    16501 ctcgcagaaa tggtttgagt tcctcaacgc tggcaaacgc attatcgcgc ccatggccga
    16561 aaggagagat gattattgcg aaagccctgg gaactgaccc ctgggttatc tggccatcac
    16621 gctaccatga tccgcagacc catgagttta tcgacagaac gcagttgatg cgtagctaca
    16681 ctaaaccgaa aaaatgagtg gtctggcggt agccccgcga acggggctgc cagctctcag
    16741 acgattactc gcctttcaca cgctcaatat ttgcacctaa agcgcgcagt ttgtcttcaa
    16801 tgcgttcgta gccacgatcg atgtgataaa tacgatcaac caccgtcgtc ccttccgcaa
    16861 tacagccagc cagcaccagg cttgctgatg cacgcagatc ggttgccata acctgtgcgc
    16921 cagaaagttt ttcaacaccg tgacaaataa cggtattgct ttcgatttcg gcatgcgcgc
    16981 ccatacggct aagctccggc acatgcataa agcggttttc aaagaccgtt tcggtgatga
    17041 atccggtccc ttctgccacc aggttcaaca gcgtgaactg ggcctgcata tcggtcggga
    17101 atgccggatg cggcgcggta cgtacgttaa cagccttcgg acgtttgcca tgcatatcca
    17161 ggctaatcca gtcttcgccg acttcgatgt ccgctccagc gtcacgcagt ttcgccagca
    17221 cggcgtcgag agtatctggc tgcgcgttac ggcagataat tttgccgcga gaaatcgccg
    17281 ccgccaccag gaaagtaccg gtttcgatac gatccggtag cacgcgatag acaccgccgc
    17341 ctaaacgttc cacaccttcg atgacgatac gatcggtgcc ctgaccgcta attttcgcac
    17401 ccagcgtaat caggaagttc gcggtatcga cgatttccgg ttcacgcgct gcgttttcaa
    17461 taatcgtggt accttccgcc agggttgcag cacacatgat ggtcaccgtt gcgccaacgc
    17521 tgactttatc catcacgata tgtgcgcctt tcagacgacc atcgacggaa gctttaacgt
    17581 aaccttcttc cagtttgatg gtcgcgccta attgttcgag gccagaaatg tgtagatcaa
    17641 ccggacgcgc accgatcgta caaccgccag gcagtgaaac ttgcccctga ccaaagcgcg
    17701 ctaccagcgg ccccagtgcc cagatagaag cacgcatggt tttaaccaga tcgtaaggtg
    17761 cgcagaatac attaacgtcg cgggcatcaa tatgcacaga accattacgt tctactttcg
    17821 cacccagctg gcttagcagc ttcattgatg tatcgacgtc tttcagtttt gggacgttct
    17881 ggatctctac cggttcttcc gccagtagag cggcaaaaag gataggcaga gcagcatttt
    17941 tagcgccgga aattgtgact tcgccctgga gcttcgttgg cccctgaaca cgaaatttat
    18001 ccatttagtt tgttctcagt taacaattca tatccgctac cggcgaatcg cccatagctc
    18061 aaaagccgtt cagtttgcga tcgcgcgccc actccgcagg ggtatacgct ttgatcgaca
    18121 cagcatgaat gcggttatcc gcaatatatt ccatcagcgg gccatagacc gtctgctgtt
    18181 ttttaacccg actcatgccg tcaaacaact cacccacggc aataacctga aagtggctgc
    18241 catcgccgga aacgtggact tcctggaggg agagagcgtt catcaacacg ctctgaattt
    18301 cattattttc catgggatct tcaatcatca gttaataaac cagcgaaaca tcttagagca
    18361 aagttgcgct ggcataaata agcaaaaagc ctcgctgata aatcagacaa ggctcgactt
    18421 gcaggcaggt ttgccggaca ggcggttaac gccatatccg gcctgaaaaa atttaacgag
    18481 gcagaacatc agcaggcaaa ttatacaatt tcgcgagggt atacactttg tcgtttaccc
    18541 cctgaagcgt cacgttgttg ccctgctttt tcgccagatc gataagatgg agcagcagtg
    18601 ccagtccacc cgtatccacg cgggacacac ggctaagatc gatgcaggta atccccttca
    18661 ccgcttcctc acgcatttcc caaagcggta gcagaacgtc ctgatccagc tctccggata
    18721 acgccagcgt gtcacccgtc tgcatccagc tcagtgactc gctcattatt ttttctcttc
    18781 cagagtgatt ttctgttgag aaatcgattt cagttgcgca gtcaggccgt cgataccttt
    18841 ggtacgcagc agcgttcccc actcgttttg tttggtggtg atcatactga cgccttcagc
    18901 aatcatgtcg taagcctgcc agtttcccgt ctgggagttt ttacgccact ggaagtccag
    18961 acgcaccggc ggacggccat tcgggtcaat aatggtaacg cgaataggca caatggtttt
    19021 atcgcccagc ggctgttctg gcgcaatctg ataggtttga ccgtgataca tcgccagcgc
    19081 ctgaccgtaa gcctgcttca ggtactcacg gaaagcggca aagtaggctt cacgttgagc
    19141 aggggtcgca ctcttgtaat actggcccag caccagcgca ccggcgtatt tcacctgtac
    19201 gtatggcagc agttcctgat caacaatggt acgcagataa tccgggttgg cccgaatttg
    19261 cggttgctca ttcttcaggc gatcgaacgt tttctgcgcc gcctcgtcca tcagcttata
    19321 aggattggtc tggtctgcct cggttgccgc actcagaggt gcaatcacca gcaaagcgac
    19381 catcattaaa cgtttaaaca tgcgtcggtt ctcctgaaat tatttcgttg tacccacagg
    19441 ttcagtggtt tcattattac ctggcgcagc agctggcgca tcgccactat tcttattgtc
    19501 atcgccttta ctaccgtaaa ggaactgacc aatgagatct tccagcacca tcgcagactt
    19561 agtgtcctga attgtatcgc catccttcag gatagcagtc cccagttccg ggtcttcaaa
    19621 accgacgttt aatgccagat attgttcccc cagcaggccg gaagtacgaa tgctcagcga
    19681 actggtatct ggaatgtggt tataacgttg ttcaatttcc agcgttacgc gcggcagata
    19741 ggttttcggg tccagcgtaa tatccgccac ccgacccaca acaacgccac caatactgac
    19801 cggagagcgc gctttcaggc cgccaatgtt atcgaacgtc gcataaagcg tgtaggtcgg
    19861 ttcagtacgt atggacgtca cgttcgccgc cttcaggcaa acaaacagcg ccgccagcag
    19921 tgctgctaat aaaaagatac ccacccaaat ttcatttttt ttcgtttgca tgaactcaat
    19981 tcccaaacat caatgcggtc agcacaaaat ccagccccag aacagccaga gacgagtgga
    20041 caacggtgcg agtggttgcc cggctaatcc cggcagacgt cgggatggcg tcgtagccgt
    20101 taaacaacga aatccacgtc accgtgatgg cgaacaccac gctcttaatc agacagttga
    20161 ccagatccat acgccagtcg acggcatttt gcattgccga ccagaagaac ccgctatcaa
    20221 tgcctttcca actgacgccg accagagatc cgccccagat ccccacggcg acgaaaataa
    20281 ccgtcaacag tggtaatgaa ataaccccag cccagaaacg gggagaaata acccgacgca
    20341 gcggatccac cgccatcatc tccatactgg agagttgctc tgtagcgcgc atcaggccga
    20401 tttctgcggt tagcgccgaa ccagcacgcc cggcaaacaa caacgcggca accaccggcc
    20461 ccagttcacg cagtagcgat aacgccacca gcatacccag actggtttcc gcactataag
    20521 tggtcagaac cagataacct tgcagcccca acaccattcc gatgaacacg ccagaaacca
    20581 caataatcag catcgacagg acgccgacat tatagagctg gcgcaccagc agcggcgcat
    20641 gtttgcgaaa ttccggtttg ccgaccagcg cattgaataa cattaacccg gcccgcccga
    20701 acgttctcag ggttttaatc cctttatgtc cgagcgacgc cagcgcattt aacagcatga
    20761 gtggcttaac tccctggtaa aagatcagcg tgataatcgc cggcaggata gcggaacgga
    20821 acaggcccgt cagctatccc gtccagaaac tgacgtacgc gcggatcagg attcgcctgc
    20881 aacgcctggg cactgccatg agcgacaatt tttttgtccg ccaggatcca ggcgtgatcc
    20941 gcaatactta acacttccgg cacatcgtga gaaaccacca cacaagtcac gcccagcgcg
    21001 ctgttcagct cagaaatcag cttcaccagt acgcccatgg tgatgggatc ttgcccaaca
    21061 aaaggttcat caaacatgat gagatccggc tccagcgcaa tcgcacgcgc cagcgctgca
    21121 cgccgcgcca tcccaccgga aagttcagaa ggcattagtt tagccgctcc acgcagcccc
    21181 acggcctcca gcttcatcat caccgtacta tgcaacaatg gcgcgggtag ttgggtatgt
    21241 tcgcgcagtg gataggcgac gttgtcaaat acgttcatat cagtgaacaa cgccccggac
    21301 tgaaataaca tgctcatccg tttgcgcact gtatacaggc gcgaacgaga catcgccgga
    21361 atattctcac catcgaaaag gatctcacca tgatctggtg cgatttgccc gccaatcaga
    21421 cggagtagcg tcgttttacc gatgcccgat ggccccatga tcgccgtgat cttccctcgc
    21481 ggcacggtca gggaaatatt atcgaagatg cagcgattgc cacgcgtaaa actgacatcg
    21541 cgcatatcga ctaaattcgc cacagactgc tccataattc acccttcgtc ttgcgttgat
    21601 tttctaagca tggcgctcaa tttaaccttg aacccaacat atttacagaa tattacccgc
    21661 cgtggttagc gaaagctggc atttgtttta ctttttagcc gcataaagtc aaaattaaga
    21721 atccgttacg gctttctgaa aatcttcagc ggaccgacga gtatacctga agaaaggacg
    21781 ttagatgctt ttagctacgg cactgttaat tgttggttta cttttggtcg tttacagtgc
    21841 cgaccgcctg gtttttgccg cgtctattct ttgccgaacc tttggcatcc cgccgctgat
    21901 catcggcatg acggtggtca gtattggtac atcgttacca gaaatcatcg tctcgcttgc
    21961 cgcgtctctg cacgaacaac gcgatttagc cgttggtaca gccctcggct caaacattat
    22021 caatattttg ctgatcctcg gtctggctgc gctggttcgt ccttttaccg tccattccga
    22081 tgttctgcgt cgtgaattac ccttaatgtt gttggtcagc gtagtggccg gttccgtact
    22141 ctatgacgga caacttagtc gcagcgatgg tatctttctc ctctttctgg ctgtgctatg
    22201 gctgctgttc attgttaaac ttgcacgtca ggctgaacgt caggggactg acagcctgac
    22261 cagagagcag cttgcagagc tgccgcgtga cggcggattg cccgtcgcgt ttttatggct
    22321 cggcattgcg cttatcatca tgccagtggc cacgcggatg gtggttgata acgccacggt
    22381 gctggcgaat tactttgcca tcagcgagtt gacgatgggt ctgacggcaa ttgctatcgg
    22441 aaccagcctg ccggaactgg caaccgcaat agcgggggtt cgcaaaggtg aaaacgacat
    22501 tgctgtcgga aatatcattg gcgcaaacat ttttaatatt gtcatcgtgt tgggtttacc
    22561 cgcgctgata acgccaggag agattgagcc attggcgtac agtcgtgact acagcgtgat
    22621 gttgctggtg agcattattt ttgcgttgct gtgctggcgg cgctccccgc aaccgggccg
    22681 tggtgtaggg gtattattaa ctggcggatt tatcgtatgg ctggcgatgt tgtactggtt
    22741 atcgccaata ctcgttgaat aactggaaac gcattatgtc gcacgtagag ttacaaccgg
    22801 gttttgactt tcagcaagca ggtaaagaag tcctggcgat tgaacgtgaa tgcctggcgg
    22861 agcttgatca atacatcaat cagaatttca cgcttgcctg tgaaaagatg ttctggtgta
    22921 aagggaaagt tgtcgtcatg gggatgggga aatcggggca catcgggcgc aaaatggccg
    22981 caacgtttgc cagcaccggt acaccttcat ttttcgtcca tcctggtgaa gccgcgcatg
    23041 gtgatttagg catggtcacc ccacaggatg tggtgattgc tatctctaac tcaggtgaat
    23101 ccagcgaaat cacggcctta attccagtgc ttaagcgtct tcacgtaccg ttaatctgca
    23161 tcaccggtcg cccggagagc agcatggcgc gcgccgcaga tgtgcatctg tgtgttaaag
    23221 tagcgaaaga agcctgtccg ttagggctgg caccgaccag cagcaccacc gccacgctgg
    23281 ttatgggcga tgccctcgct gtcgcgctgt taaaagcacg cggctttact gctgaagatt
    23341 ttgcgctctc acacccaggc ggcgcactgg gtcgtaaact tctgctgcgc gtaaacgata
    23401 ttatgcatac gggcgatgag atcccgcatg ttaagaaaac ggccagtctg cgtgacgcgt
    23461 tgctggaagt tacccgcaaa aatcttggta tgactgtcat ttgcgatgac aatatgatga
    23521 ttgaaggcat ctttaccgac ggtgatttac gccgtgtctt cgatatgggc gtggatgttc
    23581 gtcagttaag tattgccgat gtgatgacgc cggggggaat acgtgtgcgc cctggcattc
    23641 tggccgttga ggcactgaac ttaatgcagt cccgccatat cacctccgtg atggttgccg
    23701 atggcgacca tttactcgga gtgttacata tgcatgattt actgcgtgca ggcgtagtgt
    23761 aaagattcaa ggataaacaa caatgagcaa agcaggtgcg tcgcttgcga cctgttacgg
    23821 ccctgtcagc gccgacgtta tagcaaaagc agagaacatt cgtctgctga tcctcgatgt
    23881 cgatggcgta ctgtcagatg gcctgattta tatgggcaat aatggcgaag agctgaaagc
    23941 gttcaatgtt cgtgacggtt atggcattcg ttgtgcgctc acctctgata ttgaagtcgc
    24001 tatcattacc gggcgaaagg ctaaactggt agaagatcgt tgtgccacat tggggatcac
    24061 tcacttgtat caggggcagt caaacaaact gatcgccttt agcgatctgc tggaaaaact
    24121 ggcgattgcc ccggaaaatg tggcttatgt cggcgatgat ctcatcgact ggccggtaat
    24181 ggaaaaagtg ggtttaagcg tcgccgtggc cgatgcgcat ccactgttga tcccgcgcgc
    24241 cgattacgtg acgcgcattg ctggcggtcg tggcgcagtg cgcgaagttt gcgacttatt
    24301 actcctggcg cagggcaaac tggatgaagc caaagggcaa tcgatatgag taaagccaga
    24361 cgttgggtta tcattgtgct atcactggcg gttctggtga tgatcggcat tgatatggcc
    24421 gaaaaagacg ataccgccca ggtggtcgtc aacaacaatg atcccaccta taaaagcgag
    24481 catacggaca cgctcgtcta taacccagaa ggggcactaa gctatcgatt gattgctcaa
    24541 cacgttgaat attattccga tcaggccgtt tcgtggttta cgcagccggt acttaccacg
    24601 tttgataagg ataaaatccc gacatggtcc gtaaaagcag ataaagccaa gctgaccaat
    24661 gaccggatgc tctatttata tggacacgtt gaagtcaacg cacttgtgcc agactctcaa
    24721 cttcgcagaa tcacgacgga taacgcgcag atcaatctgg tgacgcagga tgttacctct
    24781 gaagacctcg tcacgttata cggaacaaca tttaactcca gcggtctgaa aatgcgcggc
    24841 aacttacgca gcaagaacgc cgagctgatt gaaaaggtta gaacatccta tgaaattcaa
    24901 aacaaacaaa ctcagcctta atcttgtgct tgccagctca cttctggccg ccagcattcc
    24961 ggcatttgcc gtaaccggag acactgatca gccgatccac attgaatcgg accagcaatc
    25021 tcttgatatg caaggcaacg tggttacctt taccggtaat gtcatcgtca cccagggcac
    25081 catcaaaatt aatgccgaca aagtggtcgt tacccgtccg ggcggcgaac aaggtaaaga
    25141 agtgattgac ggctacggta aaccggcaac gttctaccag atgcaggaca acggtaaacc
    25201 cgttgaaggt cacgcttccc agatgcacta cgaactggca aaagattttg tcgttctgac
    25261 gggtaatgct tatctgcagc aggtcgatag caacattaag ggcgataaga tcacttacct
    25321 ggtaaaagag cagaaaatgc aggctttcag cgacaaaggc aagcgcgtaa ccaccgttct
    25381 ggtgccgtcg cagctgcagg acaaaaacaa caaaggccag accccggcac agaagaaggg
    25441 taattaattc gttatggcaa cattaactgc aaagaacctt gcaaaagcct ataaaggccg
    25501 tcgcgtggta gaagacgtca gcctgaccgt caactccggg gaaattgtcg gtctgctggg
    25561 gccaaacggt gccggtaaga ccaccacttt ctacatggtt gtaggcattg tgccgcgcga
    25621 tgcgggcaac atcattattg atgatgacga tatcagtctg ctgcctctgc atgcacgcgc
    25681 gcgccgcggt atcggctatc tgccacagga agcctccatt ttccgtcgcc tcagcgttta
    25741 cgataacctg atggcggtac tgcaaattcg tgacgacttg tctgctgaac aacgtgaaga
    25801 ccgcgcgaac gagctgatgg aagagtttca cattgagcac ctgcgtgaca gcatggggca
    25861 gtcactctcc gggggtgaac gtcgccgtgt agaaattgcc cgcgcactgg ctgcgaatcc
    25921 gaaatttatt ctgctcgacg aaccgtttgc cggggttgac ccgatctcgg ttatcgacat
    25981 taaacgcatc attgagcacc tgcgcgacag cggcctgggc gtgctgatca ctgaccacaa
    26041 cgtgcgtgaa acactggcgg tttgtgaacg cgcttatatc gtcagtcagg ggcatttgat
    26101 cgcccacggc acgcctacag aaatcttaca agacgaacac gttaagcgtg tataccttgg
    26161 ggaagacttc agactctgat agggtagaag tttgcgacgt tttagcagga gagtacgatt
    26221 ctgaacatga agcaaggttt gcaactcagg cttagccaac aactggcgat gacgccacag
    26281 ctccaacagg caattcgtct gttgcagttg tcgacgctgg aacttcagca ggagctacag
    26341 caggcgctgg agagtaatcc gctgcttgag caaatcgaca ctcatgaaga aatcgacacc
    26401 cgcgaaacgc aagacagtga aacgctggac accgccgacg cgctcgaaca aaaagagatg
    26461 ccggaagagc tgccgctcga tgccagttgg gacaccattt acaccgctgg tacaccatcc
    26521 ggcaccagcg gtgactacat tgacgatgag ctgccggtct atcagggcga aacgacgcag
    26581 accttgcagg attacctgat gtggcaggtc gagctgacac cgttttccga cactgaccgc
    26641 gctattgcta cctctatcgt cgatgccgtt gatgacatcg gttatctgac tgtcccgctg
    26701 gaagatattc tcgaaagtat gggcgatgaa gagatcgaca tcgacgaggt tgaagccgtc
    26761 cttaagcgga tccaacggtt tgatccggtc ggtgtagcgg caaaagatct gcgtgactgc
    26821 ctgctaatcc aactctccca attcgataaa accacgccgt ggctggaaga ggccagactg
    26881 atcattagcg atcatctcga tctgttagcc aatcacgact tccgcacttt aatgcgcgtc
    26941 acgcgtctga aagaagatgt gctgaaagaa gccgtcaatc tgatccagtc gctcgatccg
    27001 cgccccgggc agtcgatcca gactggcgaa cctgagtatg tcattccaga tgtgctggtg
    27061 cgtaagcata acggtcactg gacggtagaa ctcaacagtg acagcattcc gcgtctgcaa
    27121 atcaaccagc actacgcctc gatgtgcaat aacgcgcgca acgatggtga cagccagttt
    27181 atccgcagca atctgcagga tgccaaatgg ttgatcaaga gtctggaaag ccgtaacgat
    27241 acgctactgc gcgtgagtcg ctgtatcgtt gaacagcagc aagccttctt tgagcaaggt
    27301 gaagaatata tgaaaccgat ggtactggcc gatatcgccc aggctgtcga aatgcatgaa
    27361 tcgacgatat ctcgcgtgac cacgcaaaaa tacctgcata gtccacgagg catttttgaa
    27421 ctgaagtatt tcttttccag tcacatcaat accgagggcg gcggcgaagc ttcctccacg
    27481 gcgattcgtg cgctggtgaa gaaattaatc gcggcggaaa acccagcgaa accgttgagc
    27541 gacagcaagt taacctcttt gctgtcggaa caaggtatca tggtggcacg ccgcactgtt
    27601 gcgaagtacc gagagtcttt atccattccg ccgtcaaacc agcgtaaaca gctcgtttga
    27661 cccaaccgat aaggaagaca ctatgcagct caacattacc ggaaataacg tcgagatcac
    27721 cgaggcactg cgcgaatttg ttacagccaa atttgccaaa cttgagcaat attttgaccg
    27781 aatcaaccag gtctatgttg ttctgaaagt ggagaaagtc acccacacct cagatgcaac
    27841 actgcatgta aacggtggtg aaattcatgc cagcgcggaa ggtcaggaca tgtacgctgc
    27901 cattgatggc ttaattgata agctggcacg ccagttgacc aaacataaag ataaactgaa
    27961 acaacactaa ttgtccgggc aattagcatg tgcatggcgg cctgttgtgc ggcacaacgg
    28021 gccatttgta cggttaatgc tccgagcctg ttccactgtt tgagtgggca ggttcttagg
    28081 tgaaattatg acaaataatg atacaactct acagcttagc agtgttctta acagggaatg
    28141 tacgcgaagc cgcgtccact gtcagagcaa aaaacgcgcc ctggaaatca tcagcgagct
    28201 ggcggcgaaa caacttagcc tgccgcctca ggtggttttt gaagctatcc tgacgcggga
    28261 aaaaatgggc agtaccggta tcggcaatgg tattgccatt ccgcatggca aactggaaga
    28321 agatactctg cgcgccgttg gcgttttcgt tcagctcgaa acgcctatag ctttcgatgc
    28381 catcgacaac caaccggtgg acttactttt tgccctgctg gtgtcggcag accaaactaa
    28441 aacgcacctg catactctgt cgctggtggc gaaacgtctg gcggacaaaa ccatctgccg
    28501 ccgtttacgc gcagcccaga gcgatgaaga gctgtatcaa atcattacgg ataccgaagg
    28561 tactccggat gaagcgtagt tattcggtaa tgtctctttt agacgttgtg aggagaaaca
    28621 gtacatggta ctgatgatcg tcagcggacg ttcaggttca ggtaaatctg tcgccctgcg
    28681 tgcgctggaa gatatgggtt tttactgcgt ggataacctt cccgtagtgt tgttacccga
    28741 tctggctcga accctggccg atcgcgagat ttctgccgcc gtcagcattg atgttcgtaa
    28801 tatgccggag tcaccagaaa tattcgaaca ggcgatgagt aacctgcctg acgctttctc
    28861 accgcaactc ctgttcctgg atgccgaccg taatacctta attcgtcgtt acagtgacac
    28921 gcgccgactg catccgcttt ccagcaaaaa cctgtcgctg gaaagtgcta tcgacaaaga
    28981 aagcgatttg ctggagcctc tgcgttcgcg agcggatctg attgtcgata cctcagaaat
    29041 gtccgttcac gagctggctg aaatgctgcg tacccgtctg ctgggtaaac gtgaacgcga
    29101 actgaccatg gtctttgagt ctttcggctt caaacacggt atccctatcg atgcagatta
    29161 cgtctttgac gtgcgcttct tgccgaaccc gcactgggat ccgaaactgc gtccaatgac
    29221 aggtcttgat aaacctgtcg ccgcgttcct cgaccgccac acagaagtac acaattttat
    29281 ctaccagacg cgaagctatc ttgagctatg gttacctatg ctggaaacca acaaccgtag
    29341 ctacttgacg gtcgccattg gttgtaccgg cgggaagcac cgttcggtgt atattgcaga
    29401 gcaactggca gactacttcc gctcgcgcgg taaaaacgtc cagtcacgcc atcgtacgct
    29461 ggaaaaacgt aaaccatgac cgtcaagcaa actgttgaaa tcacaaacaa gctgggcatg
    29521 catgcccggc ctgcaatgaa gctgtttgaa ttaatgcagg gttttgacgc tgaagtgctg
    29581 ttacgtaatg acgaaggcac cgaggccgaa gccaacagcg ttattgcgct gctgatgttg
    29641 gattctgcca aaggacggca gattgaagtt gaagcgaccg gcccacagga agaggaagca
    29701 ctggccgccg ttatcgccct ctttaactct ggttttgatg aagattaatc ttcatcactt
    29761 tgacatacaa ctcccttcaa actccccccg acaataagaa aatcacgtac tgaaatcgtt
    29821 ctcaatcaac gtcatttgta cattttgtgc gcttttcact ttcagaagaa ccttaagaaa
    29881 accttaagag gcattgttta ggttttgttt aagttaatcg accatactgg agatcgtcag
    29941 aaaatatttc caggagatgg catgattcgc ttatctgaac aaagtcccct gggcaccgga
    30001 agacatcgga aatgttatgc gcatccggaa gatgcccaac gctgtatcaa gattgtctac
    30061 catcgtggcg atggtggcga taaagagatc cgccgtgagt taaagtacta cgcgcatctt
    30121 ggtcgccggt taaaagactg gagtggaata ccgcgctatc acggtaccgt tgagacggat
    30181 tgcggaaccg gttatgtcta cgatgtgatt gcagattttg acggcaaact ctccattacg
    30241 ctgaccgaat ttgctgaaca atgccgttac gaagaagaca ttgcacaact gcgccagcta
    30301 ctgaaacagc tgaagcgtta tttgcaggat aaccgtatcg tgacgatgtc gctgaagccg
    30361 cagaatatcc tctgtcaccg catcagtgag tcagaggtta tcccggtcgt ctgcgacaac
    30421 attggtgaaa gcacattgat tccactggcg acctggtcga agtggtgttg tctgcgtaag
    30481 caagagcggt tatggaaacg atttatcgcc caaccggcgc tggctattgc cctgcagaaa
    30541 gatctgcaac ctcgtgagag caaaacgctg gccctcactt cgcgcgaagc ttaatccagc
    30601 tggtgttgtt gcataaacgg ctcaccgcct aactgataca tctgccgtaa aatccacgcc
    30661 tgacggctac gcacgtagcc cgatggcgag gagactttga aacgaagtgg attaggtaat
    30721 acagctgcca gtaatgcagc ttccgaccgg gtaagtttgc tcgcgggttt gtggaaataa
    30781 cgttgtgccg cagcttcgac gccaaacaca ccgtcgccaa attcggcgat attcaggtaa
    30841 accgtcagga tacgcttttt gctccagact gtttctatcc ccagcgttaa tccagcttcc
    30901 agcccttttc gtacccagct acgcccatcc cataaaaaga gatttttggc cgtctgctga
    30961 gaaatcgttg aagcaccgcg aatacggttt tcattgcgct cgttgtgcgc cagggctttc
    31021 tcaatggaag cgacatcaaa gccccagtgc tcaggaaatt tctgatcttc tgcggcaatc
    31081 accgccagtc ccatccacgg cgagatttga tccatactga cccagtcaga atgtgccacg
    31141 taacgaaaat tgccatgcag ccaggcgctg acctgtcgct cgaccattac cgctgagaag
    31201 ggaacaggcg caacactaaa caacgcgatg cccccgcccc agaaaacggc gaggacaacc
    31261 attaacagca aaagaaaacg gcgaacgaaa ctaaacaccg ttaagcggct tttactcatt
    31321 cagccagaac cagcacgcgg gaaaccagct tatcaatgcc gctcgccgct tctgcaatgt
    31381 tctgcgccag catatatgct ggggtggtga caatcttatt gtcttcatca accacgatat
    31441 catcgacagg acagggcaca tgctccgcgc ccatctcttc cagcacttct gcggtatcga
    31501 tatcagtacc gatggtcaaa cgcagcggga aatcgaaaat tttcggcagc atcgccgggg
    31561 caatacacat aaaaccaagc ggttttccgg cctgatgcat cgcttgtgcc agcgccttta
    31621 attcacggtc aacggtgcat tcgctaccaa gagtggcaaa attgcttaaa ttcttcgccg
    31681 cgccaaaccc ccccggcaca atcaacgcat ccagttcagc ggcatcggcc tgggccagag
    31741 gacggatttc accgcgcgtt attcgtgccg cttcaatcag cacgttgcgc gtttccgtca
    31801 tcgcttcgcc agttaaatgg ttgataacat caacctgctg cttatccggt gcaaagcaga
    31861 ccgcctgcgc accgctgcgt gaaatagcta acaacgtcaa caccgcttca tgaatttcag
    31921 aaccgtcata gacgccgcat ccgctcagaa ttacgccaat tttcttcatt gtgatcatcc
    31981 tttcgcaatt gactgaaaca cattaatttt tttaataaaa atggtacgca tcacacattt
    32041 aactgattca tgtaacaaat catttaagtt ttgctatctt aactgcgtgc ggcctgaaaa
    32101 acagtgctgt gcccttgtaa ctcatcataa taatttacgg cgcagccaag atttccctgg
    32161 tgttggcgca gtattcgcgc accccggtct agccggggtc attttttagt ggcttttgcc
    32221 acccacgctt tcagcacttc tacgtcgtga cgccactctt ctttcatctc ttcaatccat
    32281 tcaccgacgt tatcttccca ggcaggaagg tcaggagact gaatttgctg acccagctgt
    32341 tgcagatggc gtaaccccac tgaccccgcc gcacctttaa ttttatgtcc ttcctcaaca
    32401 atgccttttt tatcctgcgc cgtcagattc gactccagca cgctgacata gcccggcatc
    32461 atcttctcaa acaccgctaa cccgtcggtg atcagcttcg gtcctacaag ttcgagatac
    32521 tgttccagca tgggaatatc gagcaatgct tctgatttac tgttctcttc tgtcgtcacc
    32581 gtactctcct catcatcctg ggtatcccag aatttcttga tcatcgcggt tagcgccgga
    32641 acagaaagcg gcttactcag cacatcatcc attccagcat tgaggtactc ttgtttgtct
    32701 ttcagcacgt tagcggttaa ggccaccagc ggcggtaaat cctctcgcgg ataacgcttc
    32761 gtcagttcac gagagatatc cagcccggtc atatctggca actgaatatc cagtaacacc
    32821 aggtcgtatt cgcccggttt aaacatctct agcgccgcct tgccggtcat ggcgacatca
    32881 acgctgttac ctaatttttc cagcacagaa cgcgcaacaa tcacgttcag ttcaatgtct
    32941 tccaccagca gcacattcag cgccggtaaa ggcatatcgt cttcatcaaa cgcatcatcg
    33001 acctcttctg ctaccgacgg tgcgtggatc gtcaacgtaa aggttgaacc tttgccctgt
    33061 tcgctggtaa ccgtaatatc gccgcccata tttttcgcca gacgacgaga aacggccaga
    33121 ccaataccgg tgccggtggc aggtttaccg ccatgactgt ctttcacctg gtaatacatg
    33181 gcgaaaattt tatccagctc atcctgcgga atgccgatac cagagtcttc cacttcaaaa
    33241 tgcagcatat cgccttcatc gtagcgcacg cgcacggtaa cctggccttg ctgggtgaat
    33301 ttgacggcgt tactgatgag gttccacagg atctgccgta aacgcgtccc gtcggtaatg
    33361 acctgatgcg gtaatggcag cgtcggctcc aggttaaagc gcaatccttt ttgttgcgcc
    33421 tgcaatgcgg agagattttc cagatcggca aggaagctgg tgaaatcaac cggttgatta
    33481 tcaagctgga ccttgcgccg ttccatctta tccatgtcga taatatcgtt aaagatattc
    33541 cccagcgtga cggccgaaac atggatggtc ttgagatatt tttcctgctc ggcggtgagt
    33601 tcggtatcca gcagaatgcg gctcagaccg acgataccgt tcagcggtgt acgcaattcg
    33661 tgactgatgg tggagataaa cgtcgttttg tcgcggctgg cccgttcaag cgcatcctga
    33721 taccgcttac gctcggtaat gtctcgacca aagcccatca aaccgtgacg tttacccacg
    33781 cggtcgtagt acggcacttt acggatttca aagcaggctt tgcgcccgtc cgggtaatcc
    33841 agccactgtt cataggtcag tgacacatta tgacggaaca ctttttcatc ggtttcaatg
    33901 acttttgcgg cggcttccgg tgagtaaaca tcagcaggtt tcaggtgaac cagttgtttt
    33961 tcgctttttc cggtcagcag ctccatcgcg cggttacagc cggaaaactc tttatcttcg
    34021 ttacgataaa aaaccaggtc gggtgaagca tcaaggaagg aacgtaagaa tgaggattgc
    34081 tgctcgagct gaatttgtgt ctcttcgcgc tctttgattt caattttcag ttggccgaag
    34141 gtttcctgca gttctgcttc cgctttttca cgaacggcga tttcctgatt tagctgggca
    34201 atattatctt ttaactgaac gttgaggctc aaatcgcgct cgcgcatctc ctccagtttt
    34261 tgcaccagcc gtgacagacg ttgtcgtgac tcctccagtt gctcgacgac caccgataga
    34321 aagtagaccg cccacggcgt aatcaacaaa ccaaagaaga tagaacgaat aacatcaatg
    34381 ctttcgacct gaccatgcag caccatggtt accgccattt gtaccacaat ggcaagaacg
    34441 acgagggcca gcgccagcaa cattgagaag cgcaccagac ctaacttcat catcaggtca
    34501 acataatact gcgccagcag acgaatttgc ttcattaggg aattccttca cgacaacctg
    34561 ccccaataat acccaattct gacagttacg ttataaattg tgtgagaaat gcggaattcc
    34621 cctcaacgac ctactccgtt ggaggtagcc acggacgtcc cagcgccgat ccctgtacgc
    34681 catgttcatt cagatagcga tccagctcga ccattcccgt ccatcgattt tcgcaccaca
    34741 gaggagcaag cagcgtcggg cgacgggcgc tggcggaaat gcggtggtag atcacttccg
    34801 gtggcgtatg gcgaatcatt tctccggcag tgagcgtgta atcctccagt tcaataccgt
    34861 tcaaacgccc cgcttcccag gctttcgcca taatgctgcc tttcacaata tgcaacggat
    34921 gtagctttat accgtcaaca ccggtttcca ctacccgctc cagcgtttgc agacattcag
    34981 ccagcccttc gcctggcaga ccgacaatta aatgcgagca caccttcagc ccgcgctggc
    35041 gtgccagctg cgtcgtgcgc tgataacagg cgaaatcatg accgcgatta atgcgatgta
    35101 gcgttttgtc gtgcacggtt tgcagcccca gctccagcca cacttcgtag ccctggtctt
    35161 tatattcgca aagcagatcc agcaccgcat ccggcacgca gtccgggcgc gtgccgacac
    35221 acaaaccgac aatattggcc tggctcaccg cctgttgata catcgaacgc agcacctgaa
    35281 cttcggcaaa ggtgctggta tacgcctgga aataggcgag gtaacgtttc gcgcgattaa
    35341 ttaaattcgc ctgatgcgcc agctgttcgg caatggaacg atgctgctgc gcttcgtcgg
    35401 caaacgaggc aacattacag aaggtgcagc cgccgcgccc gatggtgccg tcgcgattcg
    35461 ggcaactgaa accgccgtgc agcgtcagct tatgcacctt ttgcccataa cgacgggtga
    35521 gatcaccacc aaacatattg actaatttct gtaactgcat aatctgatag accgcgcctt
    35581 gaaaagaggc caaagcctgc catttttagc tcaattcggc gatgacctgg atcaatcgtc
    35641 ccggcccgct tttatcaact gcataatcaa tcaaaattac cgaaatttca tgcataatca
    35701 cataaatcac ttttgcttat cttgtgtcag atttttttat ctccttatgg attttaggca
    35761 aaaacagtag catgaaacgt cattaccaat taatgcagta taaaatgctg gttttctcgt
    35821 caattcaagg caggataagg gttaacacgc ctttatgaca gtcaggaatt gactgtttct
    35881 ctaacgactt cccttttagc cttaaagata aaatctattt taatttcagc catttaataa
    35941 agaattttgc gctaaagcac atttctgtac caataagctt gccatttgac ctgtatcagc
    36001 tttcccgata agttggaaat ccgctggaag ctttctggat gagcagcctg ctcatcatat
    36061 ttatgcagta attgagatcc cctcttcacc gtattaaccg atgcgaaaag gacaacaagg
    36121 gggcgaatgc gaggcgcgcg tatgacacgc aacccccgtc gccacgctct ttctgtaccc
    36181 gtgcgcagcg gttcggtagt ggggttcccg cagagcctgg gggaggttca cgatatgttg
    36241 tacgataaat cccttgagag ggataactgt ggtttcggcc tgatcgccca catagaaggc
    36301 gaacctagcc acaaggtagt gcgtactgca atacacgcac tggcccgcat gcagcaccgt
    36361 ggcgcgattc tcgccgatgg taaaaccggt gacggttgcg gcttgctgtt acaaaaaccg
    36421 gatcgctttt ttcgcatcgt tgcgcaggag cgcggctggc gtttagcgaa aaactacgcc
    36481 gtcgggatgc tattcctgaa taaagatcct gaactcgccg ctgccgcacg ccgcatcgtt
    36541 gaagaagaac tgcaacgcga aaccttgtcg attgtgggct ggcgtgatgt ccccactaac
    36601 gaaggcgtac tgggtgaaat cgccctctcc tctctgcccc gcattgagca aatttttgtg
    36661 aacgccccgg caggctggcg tccgcgcgat atggagcgcc gtctgtttat cgcccgccgc
    36721 cgcattgaaa agcgtctcga agccgacaaa gacttctacg tctgtagcct atcgaatctg
    36781 gtgaacatct ataaaggtct gtgtatgccg gcggatctgc cgcgctttta tctggatctt
    36841 gcggacctgc gtctggaatc ggccatttgc ctgttccacc agcgcttctc cactaacacc
    36901 gtaccgcgct ggccgctggc gcaaccgttc cgctatctgg cgcataacgg tgaaatcaac
    36961 accatcactg gtaaccgcca atgggcgcgt gcgcgtacct ataaattcca gacgccgctt
    37021 atccctgatc tgcacgacgc cgcaccgttc gtcaacgaaa ccggctctga ctccagttcg
    37081 atggataaca tgctggaact cctgctggca ggcgggatgg atatcatccg cgccatgcgt
    37141 ctgttagtgc cacccgcctg gcagaacaac ccggatatgg acccggaact gcgtgccttc
    37201 tttgacttta actccatgca tatggagccg tgggatggcc cggcgggcat cgtgatgtcc
    37261 gacggtcgtt ttgccgcctg taacctcgac cgtaacggtc tgcgtccggc gcgctacgtc
    37321 atcaccaaag ataagctcat cacctgcgcc tctgaagtcg gtatctggga ttaccagcct
    37381 gacgaagtgg tcgaaaaagg ccgcgtcggg ccaggcgaac tgatggttat cgacacccgc
    37441 agtgggcata ttctgcactc ggcagaaacc gatgacgatc tgaaaagccg ccatccatat
    37501 aaagagtgga tggagaaaaa cgtccgccga ctggtaccgt ttgaagatct gcccgatgaa
    37561 gaagtgggta gccgcgaact ggacgacgac acgcttgcca gctaccagaa acagtttaac
    37621 tacagcgcgg aagagctgga ctccgtaatt cgcgtactgg gcgaaaacgg tcaggaagcg
    37681 gtcggttcga tgggcgatga taccccattc gccgtgctct ccagtcagcc gcgcattatt
    37741 tacgactact tccgccagca gtttgcccag gtgactaacc cgccaatcga cccgctgcgt
    37801 gaagcgcatg ttatgtcgct cgccaccagt atcggtcgtg aaatgaacgt cttttgcgaa
    37861 gcagagggcc aggcgcaccg tttaagcttt aaatcgccga ttctgctcta ctccgatttc
    37921 aaacagctca cgacgatgaa agaggagcac taccgcgcag atacgctgga tatcaccttt
    37981 gacgtcacta aaaccacgct cgaagcgaca gtcaaagagc tgtgcgacaa agccgaaaaa
    38041 atggtacgta gcggcaccgt gctgctggtg ctctccgacc ggaatatcgc taaagatcgc
    38101 ctgccggttc cagccccgat ggcggttggc gcgatccaga cccgtctggt cgatcaaagc
    38161 ctgcgttgcg atgccaacat catcgtcgaa accgccagcg cccgcgatcc gcaccacttc
    38221 gccgtgttgc tgggcttcgg cgcgacggct atttatccat accttgccta tgaaacgctg
    38281 ggccgcctgg tagacaccca tgcgattgcc aaagattatc gtaccgtgat gctcaactac
    38341 cgtaacggca tcaacaaagg cttgtacaaa atcatgtcca aaatgggcat ctccaccatc
    38401 gcctcttacc gctgctcgaa actgtttgaa gcggtcggtc tacacgatga tgtagtgggc
    38461 ctgtgcttcc agggggcggt cagccgcatt ggtggggcaa gctttgaaga cttccagcag
    38521 gatctgctga atctgtcgaa acgtgcctgg ctggcgcgta agcccatcag ccagggcggc
    38581 ctgctgaaat acgtccacgg cggcgaatac cacgcctaca acccggacgt ggtgcgcacg
    38641 ctgcaacaag cggtacaaag cggcgagtac agcgactatc aggaatacgc gaagctggtt
    38701 aatgagcgtc cggcaaccac gctgcgcgat ctgctggcaa ttacgccggg tgaaaacgcg
    38761 gtcaacattg ctgatgttga accggcaagc gaactgttta aacgcttcga taccgctgcg
    38821 atgtctatcg gcgcgttaag cccggaagcc cacgaggcgc tggcggaagc gatgaacagc
    38881 atcggcggta actcaaactc cggtgaaggc ggtgaagatc cggcgcgcta cggcaccaac
    38941 aaagtgtcgc gcatcaagca ggtggcttcc ggtcgctttg gggttactcc ggcgtatctg
    39001 gtcaatgccg acgtcattca gattaaagtc gcccagggcg cgaagccggg cgaaggcggt
    39061 cagttgccgg gtgataaagt cactccttac atcgccaaac tgcgctattc ggtgcccgga
    39121 gtgacgctga tctccccgcc gccgcaccac gatatctact ctatcgagga cttagcgcag
    39181 ctcattttcg acctcaagca ggttaacccg aaagcgatga tctccgtgaa gctggtttcc
    39241 gaaccgggcg taggcaccat cgcgactggc gtggcaaaag cttatgccga tttaatcacc
    39301 atcgcaggct atgacggtgg caccggcgca agcccgctgt cgtcagtgaa atacgcaggt
    39361 tgcccgtggg agctggggct tgttgaaacc cagcaggcgc tggttgccaa tggtctgcgt
    39421 cataagatcc gtttgcaggt cgatggcggc ctgaaaaccg gtgttgatat catcaaagcg
    39481 gcgattctcg gcgcagaaag cttcggcttc ggcactggcc caatggtcgc gctcggctgt
    39541 aaatatctgc gtatttgcca cctgaacaac tgcgcaacgg gtgtggcaac tcaggatgac
    39601 aaactgcgta agaatcacta tcacggcctg ccgttcaagg tgacgaatta ctttgagttt
    39661 atcgcccgtg aaacccgcga gctgatggca cagctggggg taacgcggct ggtggatctg
    39721 attggtcgca ccgacctgct gaaagagctg gacggtttca ccgccaaaca gcagaaactg
    39781 gcgctgtcga agctgctgga gaccgccgaa ccgcatccag gtaaggcact ctactgcacc
    39841 gaaaacaacc cgccgtttga taacggcctg ctgaacgcgc agttgctgca acaggcgaaa
    39901 ccgtttgtcg atgaacgcca gagcaaaacc ttctggttcg atatccgcaa taccgatcgc
    39961 tccgtgggcg cgtcgctttc aggctatatc gcccagacgc acggcgatca ggggctggca
    40021 gccgatccta tcaaagcgta cttcaacggc accgcaggcc agagtttcgg tgtatggaac
    40081 gcgggcggcg tggaactgta cctgaccggg gatgccaacg actatgtcgg taaaggcatg
    40141 gcgggcggct taatcgccat tcgtcctccg gttggttccg ccttccgcag ccatgaagca
    40201 agtattatcg gcaacacctg cctgtatggc gcgaccggtg gtcgtctgta tgccgcaggc
    40261 cgcgcgggtg aacgtttcgg cgtgcggaac tccggtgcta tcaccgtggt agaaggcatt
    40321 ggcgacaacg gctgtgaata tatgacgggc ggtattgtct gtattctggg taaaaccggc
    40381 gttaacttcg gtgcgggcat gaccggcggc ttcgcttacg ttctcgatga aagcggtgat
    40441 ttccgcaaac gcgttaaccc ggaactggtc gaggtcttaa gcgttgacga tctggcgatc
    40501 catgaagagc atctgcgcgg tcttatcacc gagcatgttc agcatactgg ctctcagcgc
    40561 ggtgaagaga ttctggcgaa ctggtcaacc ttcgccacta aatttgcgct ggttaaaccg
    40621 aagtccagtg atgtaaaagc actgctgggt catcgtagtc gtagcgcagc agagttgcgc
    40681 gtgcaggcgc agtaaggggt agcaacaatg agtcagaatg tttatcaatt tatcgacctg
    40741 cagcgcgttg atccgccaaa gaaaccgctg aagatccgca aaattgagtt tgttgaaatt
    40801 tacgagccgt tttccgaagg ccaggccaaa gcgcaggctg accgctgcct gtcgtgcggc
    40861 aacccatact gcgagtggaa atgtccggta cacaactaca tcccgaactg gctgaagctc
    40921 gccaacgagg ggcgtatttt tgaagcggcg gaactgtcgc accagaccaa caccctgccg
    40981 gaagtttgcg gacgagtctg cccgcaagac cgtctgtgcg aaggttcctg cactctgaac
    41041 gatgagtttg gcgcggtgac catcggcaac attgagcgtt atatcaatga taaagcgttc
    41101 gaaatgggct ggcgtccgga tatgtccggc gtgaagcaaa ccggtaaaaa agtggcgatt
    41161 atcggcgcag gcccggcagg tctggcgtgt gcggatgtcc tgacgcgtaa cggcgtaaaa
    41221 gccgttgtct tcgaccgtca tccagaaatt ggcgggctgc tgaccttcgg tattccggcc
    41281 ttcaagctgg aaaaagaggt aatgacgcgt cgccgtgaaa tcttcaccgg catgggtatt
    41341 gaattcaaac tcaataccga agtgggccgc gacgtgcagc tggacgatct gctgagtgat
    41401 tacgatgccg tcttccttgg cgtcgggact tatcagtcaa tgcgcggcgg gctggaaaac
    41461 gaagacgccg atggcgtgta cgcagcgctg ccgttcctta tcgccaacac caaacagtta
    41521 atgggctttg gtgaaacccg cgacgaaccg ttcgtcagca tggaaggcaa acgcgtggtg
    41581 gtccttggcg gtggcgacac tgcgatggac tgcgtgcgta cgtccgtgcg ccagggagcg
    41641 aagcacgtta cctgtgccta tcgtcgtgat gaagagaaca tgccgggttc ccgccgcgaa
    41701 gtgaaaaacg cgcgggaaga aggcgtagag ttcaaattca acgtccagcc actggggatt
    41761 gaagtgaacg gtaacggcaa agtcagcggc gtaaaaatgg tacgtaccga aatgggcgaa
    41821 ccggatgcca aaggccgtcg ccgcgcggag atcgttgcag gttccgaaca tatcgttccg
    41881 gcagatgcag tgatcatggc gtttggtttc cgtccacaca acatggaatg gctggcaaaa
    41941 cacagcgtcg agctggattc acaaggccgc atcatcgccc cggaaggcag cgacaacgcc
    42001 ttccagacca gcaacccgaa aatctttgct ggcggcgata tcgtccgtgg ttccgatctg
    42061 gtggtaaccg ctattgccga aggtcgtaag gcggcagacg gtattatgaa ctggctggaa
    42121 gtttaagctc tgtcccagcg ccgcctcctc agaaaagagt cggcgctgaa attctccttt
    42181 ctttctggaa agcgcctcgc ataatctgaa gaaaaccaat gtaatacgct catctggatt
    42241 gttaccggta gatgcccggc gtatagctga ttccattatt caacgcagcg ccatataaag
    42301 ggacagataa tggaatcgct ctcagagaga acctcaacag gctaccagca aatccacgac
    42361 ggtattattc acctggtcga tagcgcccgg acggaaacgg tacgtagcgt taacgcgctg
    42421 atgaccgcga catactggga aattggccga cgaattgtcg aatttgaaca aggtggcgag
    42481 gcaagggctg cgtatggtgc gcagctaatc aagcgactat caaaggattt aagtctaagg
    42541 tataagcgtg gtttctctgc aaaaaactta cgccaaatga ggctttttta cctctttttt
    42601 caacatgttg aaattcgcca gacagtgtct ggcgaattaa caccattggg aattccgcag
    42661 acaccatctg cagaattccc atcggcaaag atttggcaga cgctgtctgc caaattgaga
    42721 ggctgtttga tatttccaca gcttttgtat tgcatctcct attttcggtt tgtatattca
    42781 tgcaaataaa cgaaggaaag ttgccgtgat ttacattgaa cagcttgaat taatccataa
    42841 aagtggtgat gtgttgtatc cagtaaaaat aaccagaaaa tcatcaggaa aaactgcctt
    42901 ccaccttgtt ccattcggct taaataagac tcacgacctt ctagaggtgg aagatgcttc
    42961 agaagcaatt cggctggtga ttgatgaacg tcattccatt cgctgcagta cacttacagc
    43021 aactattacg aataaaaagg gcaagcgaat taagcggaca ggtatataca gtatcaaggg
    43081 tgttaacata aaagaataca acgtcaggta atggcacaga gcggtccgtc agttcaggtt
    43141 tggctctgtg ccaaagatgg gtcaggtcac acctgatcta ataaatttaa gccattaaat
    43201 cagccttaac cttcaccacc acctttttaa tctcacccgg ctcgccaaca acgcatcccg
    43261 gcttatgcgg ctctcctagc ataaacacgg cgaacattcc cggtttcagg atgatggctt
    43321 gctcgttttc aatggtgctg caaagctggt aatcatcctc atggtggaac tcttcacact
    43381 gacgcgcagt gcctgccatg ccaaacagaa tccgttcttc accgtttaat aacagctgga
    43441 tatcaatgta ttgctcgtgc agctccgctt ttttctcaac gggcgattga gtgttaaacg
    43501 tcatgacatt cataaaaata ttgtcgccct gtaattcgta acgacccggc gctttttctt
    43561 gcggtctggc agctaatgcc agcgttaacg cgtcctgtaa cgcaggatgt aacccagcag
    43621 acggtaatga ccgtacttca cccatcatca taatttttct ccctgggcca acagcgctgc
    43681 cccaagtaaa cctgcatcat ggcggtagtg cgccgccagt aaatcaacat gaaataccgc
    43741 tggctcctgc gccagatacg tttccaccaa cgccagatac ccttctgcca gaccaacgct
    43801 gccaccgacc accacgcact ggcaatcagt tgtggcttta atatcagcga tcagccttgc
    43861 cagcacatgt gcggagcggt gaatcagttg ctgcgcctgc tcgtcgccct gcccggcgcg
    43921 tgtgaaaata gttctcgcat ccgcgcccat gagttcacct tgcgctgccg ctgcaatacc
    43981 gcgaccagaa gcaatcgctt ccacgcaacc tgtgcgtcca cagccgcaga ctgggccgtg
    44041 tggatcggca agcgtatgcc cgatatgccc cgccagaccg ccagggccgg taagcagttt
    44101 accgccgctc actacaccgc cgccgacgcc agtggaaacg gtgataaaca ccatctcggt
    44161 tacatcgcct tccagcgcct gatactctgc ccatgctgcg gcctgcgcgt cgttaatggc
    44221 aatggtcggc aaattggtaa gttgttccag cgttttgact aacggaaagt gtagcaatcc
    44281 accgagatta tgcggattca gcgccagcaa actgccgtcg cgaataattc cggttgaagc
    44341 gatggcaacc cgctgcgcat gagcttgcaa cggagagact aatgcggata aggcatcacg
    44401 caaggcttct ggtgtctggc tggctggcgt aggaagttca cgacgatcgc ggatctgccc
    44461 gtcagcgcca atcagcgcgg cggcaagttt agtaccgccg atatcaatcg ccagtgtggt
    44521 catagcaccg cctttttcat cgctgtgttg taccactgac aaatgtgttc aagacgcgta
    44581 atggcagaac cgaccgtcac cgcccacgcg ccgtggcgca tcgcatctgc cgcctgagca
    44641 ggcgtgttgt aacgcccttc ggcaatcacc cgacatccgg cttcgctcaa cgttttcacc
    44701 agcgccagat ccggctcttc tggcgtttca ggcgtggtat agccagaaag cgtagtgcca
    44761 ataatttcgg cgcccagctt ttggcatgcc aggccgtctt ccggcgttga gcagtcggtc
    44821 atcgccagta aaccgtgatg atgtatacgt gccagcagcg tttcaacagg caccggacgc
    44881 gggcggtcgg tgccgtcaat ggcgataatg tccgcgcccg cctgcgccag cgcatcgaca
    44941 tcttcaatat aggccgtgat gcgtaccgga gaatcctcca gatcgcgttt cacaattcca
    45001 ataatcggca cgctcaccac cgcacgcgtg gcttgcagat ttgccacacc ttcgatgcga
    45061 atggcaaccg cgcccgcctg ctctgccgct aatgccatgg ctgcgacgat ttcgggttta
    45121 tcgagcgggc tgtcaggaat cggctggcag gagacaatca ggccaccgtt agcagcgatt
    45181 ttttgatcca gttgtgcaag taacgacata catcttccct tagcgaaagg cccggtacat
    45241 agaccgggca acaggattaa cttttggttt tgactaaatc gtttttggcg ctgccaaatg
    45301 gcacggcacc gctaaatggt ttgccgtcga tagcgtcatg agtacgcaat gcttccgggc
    45361 gcagccaacg ctgaacgcgg gaaggcatat ccagaccaat cagcaggatc accacaaatg
    45421 tcaggctgaa cgagagcgat gccagcgcag tacccagatc cagacgttga gcgatcaacg
    45481 cgccgatgat tggggccagt gcgccaccca atgcgccaac gttgtaggta aagcccaggc
    45541 ccgctgcacg ctggtcggta tcgaaataac cgccaatcag tttcggtaag atcccggcga
    45601 tcccttgtcc aagcatttgc tggaagaaca gtaacagacc gagcacccag acgtttgcgc
    45661 cgccaatcgc aaataccgga ataatcagca gctgcgaggc cagcaggcta caaacgtacg
    45721 ctttgcgggt tcccagccag tcaccgagga agcctcctac gcagcatccc acggccgcgc
    45781 caaagccact aaagaacagc acattggcga cagtatgcgg gttataagcc agatcggttt
    45841 tcagatacgt tggcagcagc gcctgaatcg gccatgagta gaggaaagca aacaacacga
    45901 ccaccatcag cattacgccc gttggccagc gtttgcctgt actctgcacc ataaagctga
    45961 taaagattgc ggcgcataac agcccaagaa cagcgacgat cgcggcattt tgcaggttac
    46021 cggcgaagca gaaccacagc gcagtagccg ccgccagtgt cattacgata ttggcaatgc
    46081 gatgttcacc acggtagaga atatccacca ttgtgcgtac tggtgcttta cctgcgtgtt
    46141 tctctttcca gtcttccgct tccgggatgt ttttacgcag ccagagagca aagatgattg
    46201 gcaaaatgcc gataaagaac agcgcacgcc aaccccagac cggaaccacc aggctataga
    46261 cctgagcggc aacgacggcc cccacagaga agcctgaaat caaaaaacca ctggctttgt
    46321 tacgcagatg ttttggccag ctttcaatga cataggtggc gctggaaccg tattcacccg
    46381 ccatccccat gccgatgacc agacgagcga taaacatggt gatgtagcct ggcgcaaagc
    46441 cgcaggccag cgtcccggcc gagaagagaa cgatgctggt gaccattgcc agacgacgcc
    46501 cgtagcggtc acccatagcg ccgagcatca ggccgccgaa ccagcgagag ataaaggctg
    46561 cggagatcag acttgccgcc tgcaccgtcg tcagcccgaa ttcaccctgt acttcggtga
    46621 gtaccagggc gattaaaacg aaatcaaaac cgtcaagcag atatcccaac caggcagcgg
    46681 aaaatgcgcg ccattgtgca cggttgagat ggcgatacca cgggatattc tgggttgtag
    46741 tactcattgt gagtctcccg cggtgggcga tgcccacacg ctttggtatg aaaattgtag
    46801 ggtacagatg cgtttatttc ccctcacccg gtaggggcga gcgaggggaa aacaactcac
    46861 ccgcgctctt gcatcaactg ctgggccagc gccttcagtt ctggcagata tttttcatct
    46921 accggtccaa acggtttgcg gcacagcggc acagaaacga catccatata atgcaggaca
    46981 gttttcaggc cgcggaatac gccagttttg atcagtaaat caatgacttt gttgcactca
    47041 gtttgcagtt tctgcgcggt ctggatatcg ccttctttca gcgccttcac gatcccctga
    47101 tatcgccagc ccatgatgtt gtaagtactg ccgataccac catcagcgcc tgccagcaag
    47161 ccggaagcga agatttcgtc gtaaccgtta tagagcacca gatcaggatg ttcacgacgg
    47221 atctgttcca tctgatagag atcgccagaa gtctgtttca gcgcaccgac gccaggcaac
    47281 gtaaccaggg tgttgatctg atccagggtc agttttaccc cactcagggc tggaatgttg
    47341 tacacaacca tcggcaaacc atccgctgaa tcaataattg cccgatagtg atcgcagtgt
    47401 tcttcaaagc tgaaaggata gtagaacggc gtgacggcgg agacggcatc gaagccataa
    47461 cgtttagccg atgccgcaag ttgttggctt tcggcggtgc tgacgcaacc gacgtgggcg
    47521 atgagtttaa tcttaccttt cgcctcttcg gcgacgattt ccagtacctg ttcacgctcg
    47581 gaaaggcttt gtacaaaggc ctcgccggtc gaaccaccca cgtataaacc gtcgatgccc
    47641 tgctgaatat tgaactgaac caggcgacgc agactcgctt tatccagtgc ttgttgttgg
    47701 tcaaaaggag tcaggagtgc agccattacg ccacgtaaat tcgttgccat aaatacctct
    47761 gaagtgatac ttgtctgata aacgatatac ctttatacct gttataccag atcaattaag
    47821 caacacccca tacagaaagc ttataatgcg atctgcttca ctaaagtggc attatttctt
    47881 tttgttggtg gtctgaccga aagcgtgcca ggtagcagag acgctgttga gatgcgattg
    47941 caacgcacga tcggcttcgt caggatcatg acggcggatc gcatcaacga tcgcaatatg
    48001 ctgttgatag ctaacgttgt tatgctcgtg cagagcctga tcagccacgg ttggacgtgc
    48061 ggcgatcagc cagtcgagca gggcaacgtg gatcgccatg aagattgggt taccagggat
    48121 ctccgccagc acgcggtgga aatcaacgtc tgaacgaatg aatgccgcgt tgttatccag
    48181 cgactgactg ttgatttcca gtgcttttgc cagcaaatcg atttgctcat cggtggcatg
    48241 ttcagccgca tagcgcacca gactggattc aaagaacaga cgtaattgtt cgaaatgggc
    48301 aatcccaccg ggatgagaaa ggaaatcttt cgccatgccg gaaaactcac cgatgatagt
    48361 gtccgcagaa ggacgcgaga cgcgagcgcg ttcgccgttg tttatttgca ccagaccttt
    48421 gcgttttaac gctgccagcg cttcgcgcac agaaggacgc ccgacgttaa agaacgccat
    48481 cagttcgcgt tcagacggta attgttcacc ttcgccaaat tcacgacggc ggatcatctg
    48541 ttccagctct tcttccacca tttcggagag ttttttacgc gccagcgggc ggctacgcaa
    48601 gttgcgacca attgcaggtg aagaatcttc ggtttgcgaa tcaaatgcgt tcataaggcc
    48661 cattctgtaa ggtcagtgtg attaacatca tcagtgacat cctatcacag gattgaaagt
    48721 aggggaaaat ggcaggtttt tctctttgtg cctcatcatt accataatta acggaataat
    48781 taactattgc gaaaaattaa tgtaacgcag ataaacacat cccgattgaa ttattcataa
    48841 gactattcac aagcattatg aatattatga atgtgttctt acaaaataat cataagcgca
    48901 tattttttaa tgaaaaatca catcacctac aattaaaaac acgacatccg caccataaat
    48961 agacttgcaa aaaatataac atcattgttt tcaatctgcc gtttatggga ttgaccgttt
    49021 tcttttgaca cggagttcaa caatgttcgg cataattata tctgtcatcg tattaattac
    49081 gatgggctat ttgatcctga aaaactacaa acctcaggtg gtgctggctg ccgcaggtgt
    49141 cttcctgatg atgtgcggag tctggttagg gttcggtggt gtactcgatc ccgccaaaag
    49201 cagcggctac ttgatcgtcg acatttataa tgaaatcctg cgcatgctgt ccaaccgcat
    49261 tgccggattg gggctgtcga ttatggcggt gggcggttat gcccgctaca tggagcgcat
    49321 aggagccagt cgcgcgatgg tgagcttgct aagccgcccg ttaaaactca ttcgctcgcc
    49381 gtatattatt ctgtcggcaa cttacgtcat cggccaaatc atggcgcagt ttattaccag
    49441 cgcctccggt ctgggtatgt tgctgatggt caccttattt ccgacgctgg tgagtctggg
    49501 agtaagtcgc ctctctgcgg tggcggttat cgcaaccacg atgtccattg agtgggggat
    49561 tctggaaacg aactccattt ttgcagccca ggtcgcggga atgaaaattg ccacatactt
    49621 cttccactac cagcttccgg tcgcctcttg cgtcattatc tcggtggcga tctcccactt
    49681 tttcgtgcaa cgcgcttttg acaaaaaaga taaaaatatc aatcacgaac aggcagagca
    49741 aaaaactctc gataatgtcc cgccgctcta ttacgccatt ttacctgtga tgccgttaat
    49801 cctgatgctc ggctcgctgt tcctcgccca cgtcgggctg atgaagtcag aactgcatct
    49861 ggtggtggtg atgttactaa gtttgacggt gacgatgttt gttgagttct tccgcaagca
    49921 taacttgcgc gaaacaatgg acgatgtgca ggcgtttttt gacggcatgg gtacgcagtt
    49981 tgccaacgtg gtaacgctgg tggtcgcggg tgaaatattt gcgaaaggct taacgacgat
    50041 tggcactgtt gatgcggtta tcaggggtgc ggagcattct ggtctgggcg gtattggcgt
    50101 gatgattatt atggcgctag tcattgccat ttgtgccatt gtgatgggct ctggcaatgc
    50161 gccgtttatg tcatttgcca gtcttattcc gaatatcgca gccggactac atgtaccagc
    50221 ggttgtaatg attatgccga tgcattttgc cacgacgcta gcgcgcgcgg tttcgccgat
    50281 tactgcggtg gtgaagcgga cagcgatccc catggcagtc ggtttcgtgg tgaatatgat
    50341 tgccacaatc acgctatttt attaagtcat taaaaagaca aaacaggccg cctgggcctg
    50401 ttttgtatta cttcacaaca cgtaatgccg gtcgaccacc gcgtggtggc tgcggaggtt
    50461 catcgtcagg atgagtgtca tcatcgtgat ctggcttgtc gccatcaata accgacataa
    50521 cggtttcgtt gtctgccgat gcctcttcat cattcatgat gctggtatct tcatcgtagg
    50581 cagcttcagg ctcaaacatc gtgcctgcgc cattttcacg ggcgtagata gccagcacgg
    50641 cagccagcgg cacagaaacc tgacgcggaa tgccaccaaa gcgcgcgtta aagcgcacct
    50701 catcattcgc cagttccaga ttgccgacag cacgcggcgc aatgttgagt acgatttgcc
    50761 cgtcacgcgc atattccata ggaacctgca cgccagggag cgtcacatcc accaccaggt
    50821 gcggcgtgag ctggttatcc agcaaccact catagaatgc acgcagcaga tagggacgac
    50881 gtggtgttag ctgtgacaaa tccatacaga ttaactccgg cccagacgca tttcacgttc
    50941 tgcttcagtt aaagaagcaa ggaaagagtc acgctcaaag acgcgggtca tatagccttt
    51001 cagctctttc gcacccgggc cgctgagctc gatacccagt tgcggcagac gccacagcag
    51061 cggagcaaga tagcaatcga ccaggctgaa ctcatcgctc aggaagtacg gcttctgacc
    51121 gaagaccggc gcaatcgcca gcagttcttc gcgcagttgc ttacgtgcgg catctgcttc
    51181 agaagctgaa ccgttgatga tggtgttcat cagcgtgtac cagtcttttt cgatgcgatg
    51241 catgtacaga cggctttcac cgcgagctac cgggtaaaca ggcatcagtg gcggatgcgg
    51301 gaaacgctca tccagatatt ccataatgat gcgagattcc cacagggtca gctcacgatc
    51361 caccagggtc ggaacgctct gattcgggtt gaggtcaatc agatcctgag gcggattgtc
    51421 cttttccacg tgttcgatct cgaaacttac acctttctca gccagcacaa tgcggacctg
    51481 atggctatag atgtcagtag gaccggaaaa cagcgtcatt accgaacgtt tgttggcagc
    51541 gacagccatg aaaacctcca ggtatagtca gaatttttac tgctaccagc caccaggtgg
    51601 ccagtcagaa gttgtgttac ccaataagga acgactctct ttgttcgaaa atcaaacaaa
    51661 aaatgagcaa tacccgacat ttgggcagaa aattggatga tagtttacca gattttgcga
    51721 ccattgtggt gagtggatgc cggaaatggg gaaaaagaga tgcgctttag tctgaaatag
    51781 ttgacttaga cccttattgg cgatgtggtt tttgttttgc ctgtctttca gacggcagca
    51841 aaaagcaact tcccattttt tacgctgatt cagaatttag ccataaaaaa acccgccgaa
    51901 gcgggttttt tcgtaaattg ttttctgccg gagcagaagc caattaacgt ttggagaact
    51961 gcggacgacg acgtgcttta cgcagaccga ctttcttacg ttcaacctga cgagcgtcac
    52021 gagtaacgaa gccagcttta cgcagttcag aacgcaggga ctcgtcgtat tccatcagag
    52081 cgcgggtgat accgtgacgg atcgcaccag cctgaccaga gataccacca cctttaacgg
    52141 tgatgtacag gtccagtttc tcaaccatgt cgaccagttc cagcggctga cgaactacca
    52201 tgcgggcagt ttcacgaccg aagtactgtt ccagagaacg ttggttgatt acgattttac
    52261 cgttgcccgg tttgatgaaa acgcgagctg cggaattttt gcggcgacca gtgccgtagt
    52321 attgattttc agccattgcc tataatcccg attagatgtc aagaacttgc ggttgctgtg
    52381 ccgcgtggtt gtgctcgtta cccgcgtaaa ctttcagttt acggaacata gcacgaccca
    52441 gcgggccttt tggcaacatg cctttaaccg cgatttcaat cacacgctca ggacggcgag
    52501 caatcatctc ttcaaagctc acttgtttga taccaccgat gtggccggtg tggtgatagt
    52561 acactttgtc agtacgcttg ttgccggtta cagcaacttt gtcagcgttc agaacgatga
    52621 tgtaatcacc ggtatctacg tgcggagtgt attccgcttt gtgcttaccg cgtaggcgac
    52681 gagccagttc agtagccaga cggcccagag ttttaccggt cgcgtcaaca acataccagt
    52741 cgcgttttac ggtttctggt ttagctgtaa aagttttcat taaaagctta cccaataaat
    52801 agttacacgt tggtgaacac ccaaacgtct tcaattgttg aggttcacac gacaaagtcc
    52861 ggcaaaccta ccccttcgaa tagcctatgc cagcacacaa aaagttttgg gaaaaaaact
    52921 ttcttgtaac gtggggtcgc aggattatag agaagtcggg gtcaaagatc gacccctttt
    52981 tgtgatttgt gacaggtttt aacccgccaa atgctcgcgc ttcagatact cttcgctttg
    53041 catctcttgc agacgtgaca ggcaacgctg gaactcaaac ttcagccgat cgccctgata
    53101 aatttcatac agcggcactt ctgcactcac cactaatttg acatggcgct cgtaaaactc
    53161 atccaccagc gcaataaagc gccgcgcttc gctctccatc aaccgcgtca taactggtac
    53221 atcaaacaac atgaccgtat gaaagagacg tgagagcgca atatagtcat gctgactgcg
    53281 ggcgtcgacg cacagcgtag taaaagagac cgccagcgtc tggttctcga cgcccattgt
    53341 cgctaatggc cgatggttga tttctaacgt cggtgaattt tctcgtttcc cccccgccag
    53401 cgccaaccat agtttatcca tttgcgcccg ggtttcatcg tggagtggcg aaagccacag
    53461 atgcgcctga gtgagtgtac gcagtcgata atcaacacca gcgtccacgt tcattacatc
    53521 acaatgctgt ttaatggcat cgattgcagg cagaaaacgc gcacgttgca agccatttcg
    53581 ataaagttcg tccggcggaa tatttgacgt cgctaccagg gtaataccgc gggcgaacaa
    53641 ggctttcatc agaccgccaa gtagcatggc atcggtaata tcagaaacaa aaaattcgtc
    53701 aaaacagagc acgtcagttt cggctttaaa gcgatcggca ataatttcca gcggatcggt
    53761 ctgcccctgt aaggcagtta gctcttcatg cacccgcagc ataaaacggt gaaagtgcag
    53821 gcgctgtttc cgctctcccg gcaggctttg atagaaaagg tccatcagcc aggttttccc
    53881 gcgtcctaca ccgccccaca tatataagcc acgcactggc gtatgctttg tgtcttcgcg
    53941 tttaccccac agcttaccga cccgcgccat tagcccactc gtcctggggg ctggtggcgt
    54001 gctattgatg agttcctgat aaataatttc caggcggctg acggcctctt tttgaacgtc
    54061 gtcgggttga tggctgcctt cattgagcgc cttcaggtat tgcgatgttg gggtaacgct
    54121 ttgcatgatc ttattgttat tccttgaata atcggtgcgc cgttgttcac ggttgacgaa
    54181 aaaaaggccg ttctacacta cgcgatatgc agtcgggatt ccacttctgt ggaattaacg
    54241 gttatagtgg cataatcagc cgcaggcatg gagcctgaag ccaacaccct acggaaacaa
    54301 aagacaacgg gagatgttca tgacctggga atatgcgcta attgggttag tcgtcggcat
    54361 cattattggt gctgtggcca tgcgttttgg taatcgtaaa ctacgccagc aacaggcgtt
    54421 gcagtacgaa ctggaaaaga ataaagctga actggacgag tatcgcgaag agctggttag
    54481 ccactttgcc cgcagcgcgg aattactgga taccatggcg cacgactatc gccagctgta
    54541 tcagcacatg gcgaaaagct ccagcagcct gctgccggaa ctgtctgctg aagcaaaccc
    54601 gttccgtaat cgtctggcag agtctgaagc cagcaacgat caggcaccgg tgcagatgcc
    54661 tcgcgactat tctgaaggcg catccggcct gctgcgtact ggcgcgaagc gcgactaatt
    54721 tatttttcgg gcgcagccat tgcgccctcc tcttctctcc ctccccgact atcatttaat
    54781 ctggtgtctc attgttagcc gtctgaaaat tcaataacat caaactgttt tgaatctctt
    54841 ttcttatcat tcaggtacga gagcaggaat aatgaaaaaa caaccccagc tgttgagtgc
    54901 attagcgtta agtgtcgggt taactctctc ggcgtcattt caggccgtcg cgtcgattcc
    54961 aggccaggtt gccgatcagg cccctctccc cagtctggct ccaatgctgg aaaaagtgct
    55021 tccggcagtg gtgagcgtac gggtggaagg aacggccagt cagggacaga aaatcccgga
    55081 agaattcaaa aagttttttg gtgatgattt accggagcaa cctgcacaac ccttcgaagg
    55141 tttaggctcc ggtgtcatca tcaacgccag taaaggctat gtgctgacca acaaccatgt
    55201 gattaatcag gcacagaaaa tcagtattca gctcaatgat gggcgcgagt ttgatgcaaa
    55261 actgattggt agcgatgacc agagcgatat cgccctgtta caaattcaaa acccgagcaa
    55321 attaacgcaa atcgctattg ccgactccga taaattgcgc gtcggtgatt ttgccgtagc
    55381 ggtcggtaac ccatttggcc ttgggcaaac cgccacctct ggcattgttt ccgcattagg
    55441 acgcagcggg ttgaatcttg aaggtctgga aaactttatc cagacagatg cttccattaa
    55501 ccgcggtaac tccggcggtg cactgttaaa ccttaacggt gagttaattg gcatcaacac
    55561 tgcaatcctt gcgcctggcg gcgggagcgt cgggattgga tttgccatcc ccagtaatat
    55621 ggcgcgaaca ctggcgcagc agcttatcga ctttggtgaa atcaaacgcg gtttgttagg
    55681 catcaaaggc accgagatga gtgccgatat cgccaaagcc ttcaaccttg acgtgcagcg
    55741 tggcgcgttt gtcagcgaag tgttgccagg ttctggctcg gcgaaagcgg gcgtcaaagc
    55801 gggcgatatt attaccagcc tcaacggcaa accgctgaat agctttgctg agttgcgctc
    55861 tcgtatcgcg accaccgagc cgggcacaaa agtgaaactt ggcctgctgc gtaacggcaa
    55921 accactggaa gtagaagtga cgctcgatac cagcacctct tcgtcggcca gcgctgaaat
    55981 gatcgcgcca gcgctggaag gtgcaacgtt gagcgatggt cagctaaaag atggcggcaa
    56041 aggtattaag atcgatgaag ttgtcaaagg aagcccagct gctcaggctg gcttgcaaaa
    56101 agacgatgtg atcattggcg tcaaccgcga tcgggtgaac tcgattgctg aaatgcgtaa
    56161 agtgctggcg gcaaaatcgg ccatcatcgc cctgcaaatt gtacgcggca atgaaagcat
    56221 ctatctgctg atgcgttaat gtcgtaaacc gggcatcagg cttacgtgtg atgtccggtt
    56281 aactcgtggt atgctgctgc cgttcccttt tttaatgacg cctccatcat gtttgtgaag
    56341 ctcttacgtt ccgttgcgat tggattaatt gtcggcgcta ttctgctggt tgccatgcct
    56401 tcgctgcgca gccttaaccc gctttccact ccgcaatttg acagtaccga tgagacgcct
    56461 gccagctata atctggcggt tcgccgcgcc gcgccagcgg tggttaacgt ttacaaccgt
    56521 ggtttgaaca ccaactctca caaccagctt gagatccgca ccctgggatc cggtgtaatc
    56581 atggatcaac gcggttatat catcaccaat aaacacgtca tcaacgacgc cgatcagatc
    56641 atcgtcgcct tacaggatgg tcgtgtattt gaagcattgc tggtgggatc tgactctcta
    56701 accgatctgg cggtacttaa aattaatgcc actggcggtt tacctaccat tccaatcaat
    56761 gcacgtcgcg taccgcatat tggcgacgtg gtactggcga tcggtaaccc gtacaacctc
    56821 gggcagacca ttacccaggg gattattagt gccacgggtc gaattggtct gaacccgacc
    56881 gggcggcaaa acttcctgca aaccgatgct tccattaacc acggtaactc tggcggcgcg
    56941 ctggtgaact cgctgggcga actgatgggc attaacacgc tgtcgtttga taagagtaac
    57001 gatggcgaaa cgccggaagg tatcggcttt gcgattcctt tccagttagc aaccaaaatt
    57061 atggataagc tgatccgcga tggtcgcgtg atccgcggct acattggcat tggcggacgt
    57121 gagatcgcac cgctgcacgc acagggcggt ggtatagatc aactgcaagg gatcgtggtt
    57181 aatgaagtgt cacctgacgg cccggcggcg aatgcgggta ttcaggtcaa cgatctgatt
    57241 atttcggtgg ataacaaacc ggccatctct gctctggaga cgatggatca ggtggcggaa
    57301 attcgccctg gttcggtgat cccggttgta gtgatgcgtg atgataagca gttaacgctg
    57361 caggtcacca ttcaggaata tccggcaacc aattaagtcg agtgctcaaa acaaaaaacc
    57421 ggagtctgtg
//
LOCUS       DAESHI010000014        56347 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.014, whole genome shotgun sequence.
ACCESSION   DAESHI010000014 DAESHI010000000
VERSION     DAESHI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 56347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56347
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.014"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..1334)
                     /locus_tag="KW011_000875"
     CDS             complement(<1..1334)
                     /locus_tag="KW011_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HBH7659292.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAA"
     gene            complement(1380..2138)
                     /locus_tag="KW011_000876"
     CDS             complement(1380..2138)
                     /locus_tag="KW011_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659293.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(2170..2883)
                     /locus_tag="KW011_000877"
     CDS             complement(2170..2883)
                     /locus_tag="KW011_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBH7659294.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            3057..3749
                     /locus_tag="KW011_000878"
     CDS             3057..3749
                     /locus_tag="KW011_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBH7659295.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPRPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(3798..5297)
                     /gene="pitB"
                     /locus_tag="KW011_000879"
     CDS             complement(3798..5297)
                     /gene="pitB"
                     /locus_tag="KW011_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HBH7659296.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(5585..7444)
                     /gene="gss"
                     /locus_tag="KW011_000880"
     CDS             complement(5585..7444)
                     /gene="gss"
                     /locus_tag="KW011_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HBH7659297.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            7649..8515
                     /gene="yghU"
                     /locus_tag="KW011_000881"
     CDS             7649..8515
                     /gene="yghU"
                     /locus_tag="KW011_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HBH7659298.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(8638..8886)
                     /gene="hybG"
                     /locus_tag="KW011_000882"
     CDS             complement(8638..8886)
                     /gene="hybG"
                     /locus_tag="KW011_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HBH7659299.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(8899..9240)
                     /gene="hypA"
                     /locus_tag="KW011_000883"
     CDS             complement(8899..9240)
                     /gene="hypA"
                     /locus_tag="KW011_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBH7659300.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(9233..9721)
                     /gene="hybE"
                     /locus_tag="KW011_000884"
     CDS             complement(9233..9721)
                     /gene="hybE"
                     /locus_tag="KW011_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HBH7659301.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(9714..10208)
                     /locus_tag="KW011_000885"
     CDS             complement(9714..10208)
                     /locus_tag="KW011_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HBH7659302.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(10208..11911)
                     /gene="hybC"
                     /locus_tag="KW011_000886"
     CDS             complement(10208..11911)
                     /gene="hybC"
                     /locus_tag="KW011_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HBH7659303.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYGGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(11908..13086)
                     /gene="hybB"
                     /locus_tag="KW011_000887"
     CDS             complement(11908..13086)
                     /gene="hybB"
                     /locus_tag="KW011_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HBH7659304.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(13076..14062)
                     /gene="hybA"
                     /locus_tag="KW011_000888"
     CDS             complement(13076..14062)
                     /gene="hybA"
                     /locus_tag="KW011_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HBH7659305.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(14065..15183)
                     /gene="hybO"
                     /locus_tag="KW011_000889"
     CDS             complement(14065..15183)
                     /gene="hybO"
                     /locus_tag="KW011_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HBH7659306.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(15372..15659)
                     /locus_tag="KW011_000890"
     CDS             complement(15372..15659)
                     /locus_tag="KW011_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HBH7659307.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(15778..16665)
                     /locus_tag="KW011_000891"
     CDS             complement(15778..16665)
                     /locus_tag="KW011_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBH7659308.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            16822..17862
                     /gene="gpr"
                     /locus_tag="KW011_000892"
     CDS             16822..17862
                     /gene="gpr"
                     /locus_tag="KW011_000892"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HBH7659309.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(17902..18396)
                     /locus_tag="KW011_000893"
     CDS             complement(17902..18396)
                     /locus_tag="KW011_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HBH7659310.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            18587..19471
                     /gene="yghA"
                     /locus_tag="KW011_000894"
     CDS             18587..19471
                     /gene="yghA"
                     /locus_tag="KW011_000894"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBH7659311.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(19631..20056)
                     /gene="exbD"
                     /locus_tag="KW011_000895"
     CDS             complement(19631..20056)
                     /gene="exbD"
                     /locus_tag="KW011_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HBH7659312.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(20063..20797)
                     /gene="exbB"
                     /locus_tag="KW011_000896"
     CDS             complement(20063..20797)
                     /gene="exbB"
                     /locus_tag="KW011_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBH7659313.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            21049..22236
                     /gene="metC"
                     /locus_tag="KW011_000897"
     CDS             21049..22236
                     /gene="metC"
                     /locus_tag="KW011_000897"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HBH7659314.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            22376..23035
                     /locus_tag="KW011_000898"
     CDS             22376..23035
                     /locus_tag="KW011_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBH7659315.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(23075..23974)
                     /gene="yqhC"
                     /locus_tag="KW011_000899"
     CDS             complement(23075..23974)
                     /gene="yqhC"
                     /locus_tag="KW011_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HBH7659316.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESALQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            24168..25331
                     /gene="yqhD"
                     /locus_tag="KW011_000900"
     CDS             24168..25331
                     /gene="yqhD"
                     /locus_tag="KW011_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBH7659317.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            25436..26263
                     /gene="dkgA"
                     /locus_tag="KW011_000901"
     CDS             25436..26263
                     /gene="dkgA"
                     /locus_tag="KW011_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HBH7659318.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            26463..27389
                     /locus_tag="KW011_000902"
     CDS             26463..27389
                     /locus_tag="KW011_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HBH7659319.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            27440..27697
                     /gene="yqhH"
                     /locus_tag="KW011_000903"
     CDS             27440..27697
                     /gene="yqhH"
                     /locus_tag="KW011_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HBH7659320.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(27740..29959)
                     /locus_tag="KW011_000904"
     CDS             complement(27740..29959)
                     /locus_tag="KW011_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBH7659321.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(30070..31482)
                     /gene="ftsP"
                     /locus_tag="KW011_000905"
     CDS             complement(30070..31482)
                     /gene="ftsP"
                     /locus_tag="KW011_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HBH7659322.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(31557..32294)
                     /gene="plsC"
                     /locus_tag="KW011_000906"
                     /gene_synonym="parF"
     CDS             complement(31557..32294)
                     /gene="plsC"
                     /locus_tag="KW011_000906"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HBH7659323.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(32527..34785)
                     /gene="parC"
                     /locus_tag="KW011_000907"
     CDS             complement(32527..34785)
                     /gene="parC"
                     /locus_tag="KW011_000907"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBH7659324.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            complement(35331..35813)
                     /locus_tag="KW011_000908"
     CDS             complement(35331..35813)
                     /locus_tag="KW011_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH7659325.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(35866..36258)
                     /gene="ygiW"
                     /locus_tag="KW011_000909"
     CDS             complement(35866..36258)
                     /gene="ygiW"
                     /locus_tag="KW011_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HBH7659326.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            36410..37069
                     /gene="qseB"
                     /locus_tag="KW011_000910"
     CDS             36410..37069
                     /gene="qseB"
                     /locus_tag="KW011_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HBH7659327.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            37066..38415
                     /gene="qseC"
                     /locus_tag="KW011_000911"
     CDS             37066..38415
                     /gene="qseC"
                     /locus_tag="KW011_000911"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HBH7659328.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            38525..39106
                     /gene="mdaB"
                     /locus_tag="KW011_000912"
     CDS             38525..39106
                     /gene="mdaB"
                     /locus_tag="KW011_000912"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HBH7659329.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            39137..39451
                     /locus_tag="KW011_000913"
     CDS             39137..39451
                     /locus_tag="KW011_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBH7659330.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(39499..41391)
                     /gene="parE"
                     /locus_tag="KW011_000914"
     CDS             complement(39499..41391)
                     /gene="parE"
                     /locus_tag="KW011_000914"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBH7659331.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(41420..42001)
                     /gene="yqiA"
                     /locus_tag="KW011_000915"
     CDS             complement(41420..42001)
                     /gene="yqiA"
                     /locus_tag="KW011_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HBH7659332.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(42001..42828)
                     /gene="cpdA"
                     /locus_tag="KW011_000916"
     CDS             complement(42001..42828)
                     /gene="cpdA"
                     /locus_tag="KW011_000916"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HBH7659333.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(42853..43275)
                     /locus_tag="KW011_000917"
     CDS             complement(42853..43275)
                     /locus_tag="KW011_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HBH7659334.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(43276..43905)
                     /gene="nudF"
                     /locus_tag="KW011_000918"
     CDS             complement(43276..43905)
                     /gene="nudF"
                     /locus_tag="KW011_000918"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HBH7659335.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            44110..45591
                     /gene="tolC"
                     /locus_tag="KW011_000919"
     CDS             44110..45591
                     /gene="tolC"
                     /locus_tag="KW011_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HBH7659336.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            45739..46410
                     /locus_tag="KW011_000920"
     CDS             45739..46410
                     /locus_tag="KW011_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HBH7659337.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            46416..47576
                     /locus_tag="KW011_000921"
     CDS             46416..47576
                     /locus_tag="KW011_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBH7659338.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(47614..48402)
                     /gene="ygiD"
                     /locus_tag="KW011_000922"
     CDS             complement(47614..48402)
                     /gene="ygiD"
                     /locus_tag="KW011_000922"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HBH7659339.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            48545..49318
                     /gene="zupT"
                     /locus_tag="KW011_000923"
     CDS             48545..49318
                     /gene="zupT"
                     /locus_tag="KW011_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBH7659340.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(49376..49546)
                     /locus_tag="KW011_000924"
     CDS             complement(49376..49546)
                     /locus_tag="KW011_000924"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659341.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(49807..50460)
                     /gene="ribB"
                     /locus_tag="KW011_000925"
     CDS             complement(49807..50460)
                     /gene="ribB"
                     /locus_tag="KW011_000925"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HBH7659342.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(50564..50712)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            50834..51124
                     /gene="ubiK"
                     /locus_tag="KW011_000926"
     CDS             50834..51124
                     /gene="ubiK"
                     /locus_tag="KW011_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HBH7659343.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
     gene            51407..51958
                     /locus_tag="KW011_000927"
     CDS             51407..51958
                     /locus_tag="KW011_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HBH7659344.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            52018..54522
                     /locus_tag="KW011_000928"
     CDS             52018..54522
                     /locus_tag="KW011_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBH7659345.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYMNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSASLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            54538..55287
                     /locus_tag="KW011_000929"
     CDS             54538..55287
                     /locus_tag="KW011_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBH7659346.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            55289..>56347
                     /locus_tag="KW011_000930"
     CDS             55289..>56347
                     /locus_tag="KW011_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659347.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITY"
BASE COUNT        14373 a        14723 c        13825 g        13426 t
ORIGIN      
        1 gctgcgttat aaagcaagga atgacgctga aaaaatgcct ttaaagttgg aattaacgag
       61 agcaccacat ccagacattg cccaccgaag taagcaaacg ctgctcccag cacaccaaac
      121 agatgacaga acatgaacag caatacgatg tagccgattg aggcgatggt ttgcgccacg
      181 agaaaagcgc gctgttttcc cgccatcaat agcaatgact cctgcgggaa acccagcatc
      241 gaaatcacaa ttgcgcccag catcacctga atcaggtcat acgcttcgag atacttaacg
      301 ccaaacacca gcgaaatgag cggcttgccg gcgatcaaca ccgcgagcgc caccagaata
      361 ccgattccgc ccgccagtaa accggacttc acacctaaca accacggtct ggtggtgcgc
      421 ggatctaaac gcatcacctc tgggtaaaaa cttttaccca gcaaacccgc tggcgttccg
      481 gcggcgtcga agaatgtcat ggcgatttta aataacccgg cggcagcggg tcctaacacg
      541 atccccacca gcactgtgct gcacgagtta cgcgccgacc agatggagtg ggcaatgttg
      601 gttgaccaga caaaactcca cgcgcctttg atgtgtcggg cagactcaaa cagattcaat
      661 ttgaaggcat tatggatatt tctgcggcgt aattcgcgcg ctgcaaacca ccagtacatg
      721 gtgccgccaa ccagattcga cacgtaccag gcaataacaa aacccgcaaa accaaagtca
      781 aaataccagg ctacgacgct ccccgctgcg cgcagaaaag gtttcgtcgc ctgctgtaca
      841 gcaattaaat cgaagcgatc taccgcacgc agaatgccgg tcggcgtgga ggaagccatt
      901 gaaggaatga gcgtgcaata cagcgccgcc agccaaaagc tttggtcatc cagacctaat
      961 gaatgggaaa ggaatggcag taaggcaatg ccgccgacaa tcgccaccgc gccgctgacg
     1021 atatccagcg agaaggaaaa tgagacgaca ttgcgaaatt gctgcggatt attgttggtc
     1081 aaggctggcg ttccgtactg aaccaccagc tgccatgtct gaaacttaat aaaatcgctg
     1141 atcgacttgg cgtacgattg cacaatcacc agtacgccaa acatggcggg cgtcatccct
     1201 ttaccggcac acgagagcgc caacagcccc agcaaggcgc tcacgacatt actggagcct
     1261 aaccaggcgc tattgcgaat aatggtgcga aacgcgccat ctgcaaacca atgtttgatg
     1321 ttaaaacccg ccaattcagc ctgaccttat cgttgatagt aaaaagtatc ccgccagcct
     1381 taagttaaac ttcggcggtc agaaacgatg gcaaccagag aaaccgcctt ctgtgcctgt
     1441 tccagcactt cgctgtaggg cgctctggaa tcaatctcaa gaatttttgt gccgttatag
     1501 ccaatcttcg acatgacacc gattttgtcc tgcagctcgg catagtcatg gtcaggcttg
     1561 cgggagatgg cagtctcaat atcaatgccc aggcgaataa ttaattccgg gcgatattgc
     1621 gccatttgtt ggtataaacg ccgttcgcgc tgcgccagaa acatgctgat tttcccggtc
     1681 gcacgttcga cgccaatccc cggtccgtcg taataaaagc ccgaaatttc agcctgcggg
     1741 aagcgatcgc tgaccaccag aacgccactt tgcgccagtc gctgaacctt gcgtagattc
     1801 gccattcttc gcagcgagaa gcagtacata atcaccgccg cccatagcgc cggagatttg
     1861 gttttcatgc tttgggtttt cgaggatttg gccgccagtc gccgttccag ccagacgcca
     1921 accaacggta atcgtttgat tttgtcgccg tcttcgcccg agagcagccc cagatagcgc
     1981 cgctcggttt gccagtgctg ttgcaatgat ttcaccagat cggtggtcag cgtggattta
     2041 ccggtaccat cgcacccgac caccgcaatt agccccggaa tgtagctggg ctgcggcgcg
     2101 gttgtactat taacagtttg tgtttgtagt gcatccattc gttaaatcat cccctatcca
     2161 ttttggtgat caggaaagcg cggcgtgaat tgcacgtagt gacgcttgca ggatttcatc
     2221 ggctggttgc cgaccatcca gttcaaggat cctggcgccg ttgaaagtca gttgcggcgt
     2281 aacggcgatt ttttcctgta acgctgccag ttggtggtca ggtttacgcg caaacgcggt
     2341 ttgttcatca atgccaagac gaatcaacaa tacgggcaaa taagatgcca tccattggta
     2401 cagcttcagc tcgcgctgcc ttaacatttt tatccaaccg ttaccgcccg tggtttttgc
     2461 caattgcggg ccatcaaagc gaaaacccgg cacttcaacc tgcgggtagc ggtcggtgat
     2521 gagcagaaag ccttgctggc ttttacacaa cattttgcga aacttgtacg cccgccagca
     2581 ggaaagcaga tagataacca gagcagtaat attgccaggc ggtgttgagg gtttttcgtg
     2641 cacatgtgcc gctttacttc gcagataacg cccaaaaggt gcgccaataa cagggagctg
     2701 tgaaatccat tcgccaatta gcccggacga ttgcccgaga taaatgtgtt ctgttggcat
     2761 tcttgctgcc agttcattta ccaggcttgc cgtgagggtc gatttacctg aaccatcaca
     2821 tcctacaatg gcaataacac gcactggcgt ggaattaatt attgacatat tacgttgatt
     2881 cacgaaaaac ccggcagtaa ataatgtatt gaatattacg tggtccgtgc tcagaatatc
     2941 cgttcaacct tattgtgaca atggttaatt gtgacattga cctgaattac gctttcataa
     3001 aaacatatta accaaataaa tatttttaat ggatatttaa attaaaggat atattcatgc
     3061 agtcaataac acctccatta attgccgtta ttggtagcga tggttcaggc aagtcaacgg
     3121 tgtgtgaaca tcttattacc gttgtcgaaa aatatggtgc tgccgaaaga gttcatttag
     3181 gaaaacaggc cggaaatgtc ggtcgtgcag tgacaaaatt accgttgatg ggaaaatcct
     3241 tacataaaac aattgaacga aatcaggtga aaacagcaaa aaaattgcct agaccagttc
     3301 cggcgctggt aattacagcg tttgtcgccc gtcgcttact gcgctttcgt catatgcttg
     3361 cctgtcgtcg tcgcgggtta attgttctaa ccgaccgtta tcctcaggac caaattcctg
     3421 gcgcttacga tggtacggtg ttcccaccta acgttgaagg tggtcgtttt gtctcatggc
     3481 tggcaagcca ggaacgtaaa gcgtttcact ggatggcgag ccataagcct gatctggtca
     3541 tcaaactcaa tgttgacctt gaagttgcct gtgcacgtaa acccgaccat aaacgggaat
     3601 cgctggcgag gaagattgcc ataacgccac agttaacctt tggtggtgca caactggttg
     3661 atatcgatgc caatcagcca ctggaacagg tgttggttga tgcagaaaaa gcgattacgg
     3721 attttatgac cgcgcgtggt tatcactagt caaaaatgga aatgcccgat cgccaggacc
     3781 gggcattttc aggaaggtta aatcaactgc aatgctatcc agtacagccc accagaaaga
     3841 aaaattgccg ccggtaaagt aaacacccac gccatcagga tgctggttac cgttttacgc
     3901 tgtaacccac cgccgtccac caccatcgtc cctgctaccg cagacgagag gacgtgtgtt
     3961 gtggagacag gcatcccaat ataactggca aggccgatag acactgccgc cgtcatttgt
     4021 gccgccatgc cttgcgcata cgtcatgccg cgcttaccaa tcttctcacc gatggtcatc
     4081 gctacacgac gccagccaat catggtgcca atgccgagcg ccagtgctac cgccatgatg
     4141 atccacacag gagcgtactc aatggtgctt aacatatcgc tgcgcagttt tttcagcagg
     4201 ttctggtctt ctttactgac gcctggcagt ttcgccagct tcgcggaggt atcagagatg
     4261 cacagcataa tgcggcgcag ctggctgcgc tgactcacgc ttaacggctc gtaactttcc
     4321 atattgcctg gcagcatcgt tttaacgcgc gcaatcgcat caaaggtatt tgccggatga
     4381 cagtgaaact ctgttacttg cgtgccatca gtcgatgctg caggcaatgg aggttccatc
     4441 gcaatcaact tctgcggcag ttcaggatgc tgttgcaggt agtgttcgaa gttggtgacg
     4501 gcatcgcggg tacgggtaat ttcatagccg gacgcgttca tattgacgac gaagccagca
     4561 ggggcaatcc ccaccagtac cagcatcacc aggccgatcc ctttttgtcc gtcgttcgcg
     4621 ccgtgcgaaa acgccacgcc cgcagcggaa acaatcagcg caatacgcgt ccagaatggc
     4681 ggtttacgtt tgcctttttt ctttttgcga tcttccggaa tgcggtgaat acggtcacgc
     4741 tttttcgtcc cgctccagta gcgtcgcagc aggaatatca ggcctcccgc aatgaccagg
     4801 ccgacgatag gggaaacaat cagcgaggag aaaattttgg tcacttcacg caggtttaac
     4861 gcatccatca ccgatgatcc ggttaacagc gcgttggtta aaccgatgcc gataatcgca
     4921 ccaatcaagg tgtgcgaact ggaggccggt aaaccgaaga accacgttcc caggttccag
     4981 ataatcgccg ccagcagcat ggaaaagacc atcgccaggc cgtgggttga ccccatattc
     5041 agcaacaaat cggttggcaa catatggaca atggcatagg caacgctaag tccgcccaat
     5101 aacacgccaa aaaagttaaa aaatgccgcc atcaccacag caagttgtgg ttgcatggca
     5161 cgagtataaa taacggttgc caccgcattc gccgtgtcat gaaaaccatt gattgcttcg
     5221 tagaacaaca caaatgccag agcaagcaat aacaaaagcc ctgtgtatat atcaaggcca
     5281 acaaataaat ttagcatata ttagattacg ccattttgaa tttacgaacg gacgcattat
     5341 cagtgacttt aacggcatgg gcaaagtgaa atatcatttt ttaacgataa aaagagtgtt
     5401 atttcttgtg tatcagacaa taagcattca ttcaaaacct ttagaaataa tcaaaacaca
     5461 ggtattccac tggttaatat attcacatat gaaataaata ttagaacata taaatattga
     5521 atattttgat taatccccgt actgattatt cttcataatc agtacggggt tgctacaaca
     5581 tgtattactt tttcaccaca attaacggtt caatatcact ctcttttttg atgaccagtg
     5641 attcatcacc gcgcaaacac gtcccaccgt agttgccgcc cacggtgaag gtacatacct
     5701 gaatgtattt accgtccact ttcggcaaac accacagttg ctgatagatg tttttctgct
     5761 cggcaaattt accgctggtt ttgtccagca cctcttcatg atggctgacc aggtcgatat
     5821 tgctaccaca gcgaccggca atcggtttca ctgcgtaacc tgttttcacc aattcatcat
     5881 taacagtgaa atcggtatcc agcaggtaac gatggtgcgg gaacagcgac cagaggatcg
     5941 gcagaatcgc tttgttgccg gggatcaccg tccacagcgg ctcaaagact agcacttccg
     6001 ggcgcagcaa tacgtcgata agacgcactt cgttttgcgg atgaccggta cggattggca
     6061 ccgcagcaaa ctcacggtcg ctaacttcac gaatctgatc aaacgcggtt tcccacgccc
     6121 aggttttcca cacgcagtta accagtcgcc cttccccatc aatcagttgc ccggcagcat
     6181 cccagcccag ttcatccaat ccacgcaaga tacgcgtttc aaagcccgcc tggtgcagcg
     6241 cctgctccat aaactgcgcg tgatagtttt cctcgatatc tttgtcctgc atgatatgga
     6301 caaacggacg tgcacgactg tgtttccagg caccagccaa ttcgttaatc agcccttccg
     6361 ccggattgaa gccgttgcct ttatagccct gctccgccca acgttcgagg atcaagcccg
     6421 cttcggtatg acaggaggcg gagtcggcgt tgtactcgta aaccttcagg ccacgctcat
     6481 ccatgcagaa atccatacga ccagtgatca tatggtgacg gcgacgctgc caggagagac
     6541 gcaaacgtgg ccagaggatt ttcgggatgt cgaacagcgc cagcaggttg tcatctttca
     6601 gcaccttgtc ggttgcgtga agatacatca ggtgcagctc gttggtggct ttaattagct
     6661 cctgctcggc actctcggta atggtgtagt aatgataagg atcctgattg atcacctgac
     6721 cgttggcctg cacataggcg ttttgcagcg gatctttttc atccagccat ttaccgtcaa
     6781 actggccttt gttttccagg cgcgctccgc tgattttcag cagctcgcct gcaatttccg
     6841 gctgcggtaa gctgtattca gtatcttccg tctggatcat ccagcccaga atggtggtgt
     6901 catcaaaagt gtctttcagg gtatagcagc cgttttcgac caccatctcc agctcacgcg
     6961 tccactgttg cccttgcggc aacggggaat gaatcacgtt ctgttccgca atacggactt
     7021 tgttgccatg caattgggta atgatggcga catggccagt gtctttaaat tcaccgcctt
     7081 tatcccagat aagaagcgca cccgcgaccg gcgcacgcgg cgagccgtta ggaaatgcct
     7141 gcaatggcag gatgttgtca ttaaccactt ctcgcaggaa gcgcagcgag aaaatctccc
     7201 acgccatacc cacgtcagta aagaccacac cgtaattcag aaagagaaaa cggcgagcaa
     7261 attcaacgca ttgccacttg tggcccatat attcgtcgtc gatatagcta cggaatacgg
     7321 cgtcatcttc gtattcctgc ggatcgagag aactgtaatc tgaagagtag attgctaccc
     7381 cacctggggc gtagcccaat aatgtcccga acggggcatc ctggctggtc gttcctttgc
     7441 tcatcacttt acctttaaca atacaaccta agcagttggc gtaattgttg tgctctgatt
     7501 acctgctcag cgacattaac cggacagagg tcagactaat catacacctc atcgcagcgg
     7561 ctggcgcaaa gggttaaaaa attcacattc tgtacagcaa gtgacctgct acactgcttc
     7621 aacactacca ctcagaaggc aactcactat gacagacaat acttatcagc ccgcgaaagt
     7681 ctggacgtgg gataaatccg ctggcggcgc gttcgccaat atcaatcgcc cggtttctgg
     7741 tccgacgcat gaaaaaacgc tgcccgttgg caaacaccca ttgcaacttt attcgctggg
     7801 aacgccgaac ggtcagaaag taacgattat gcttgaggag ctgctggcgc tgggcgttac
     7861 tggtgcagag tacgacgcct ggctgattcg tattggcgat ggcgatcaat tctccagcgg
     7921 ctttgtcgaa gtgaacccaa actcgaagat cccggcgctg cgcgatcata cgcataatcc
     7981 gccgatccgc gtgtttgaat ctggttcgat cctgctttat ctggcggaga aatttggcta
     8041 cttcctgccg caggatttgg caaagcgtac tgaaacgatg aactggctgt tctggttaca
     8101 gggcgcggca ccgttcctcg gcggtggttt tggtcacttt taccattacg cgccgatgaa
     8161 aattgagtac gccatcaacc gctttaccat ggaagccaaa cgtctgctcg acgtgctgga
     8221 taagcaactg gcgcagcata agtttgttgc gggcgatgag tacaccattg cggatatggc
     8281 gatttggccg tggtttggca atgtggtgtt aggcggcgtg tatgatgccg cagagtttct
     8341 tgatgcgggc agttataagc atgtacaacg ctgggcgaaa gaagtaggcg aacgtccggc
     8401 ggtgaaacgt gggcgtattg ttaaccgcac caacggaccg ctgaatgagc agttgcatga
     8461 gcgccatgac gccagtgatt tcgagacgaa tacggaagat aagcgtcagg ggtaagggtt
     8521 ggtgttcgtc gcagcaagcc atccaggccg gataaggcgt tcacgccgca tccggcaatc
     8581 gtgcacaatg cctgatgcga cgctgccgcg tcttatcagg cctacgaaag ctaatcatca
     8641 cgcgctggta atgtcgtaat ccatttggcg cagtgcgtct aatgtggctt tggcttcatc
     8701 ttcgtcgatg atgctcatgg caaatccgac gtgtaccagc acccactggc ccagtagatc
     8761 ggcagggtta ccttcacaaa tcagggcgat attcacgtcg cgcttgatac cacatacttc
     8821 aacctgcgca agctggtgaa tatcttcacc gacagccagc acctggcctg gaacgccaat
     8881 acacattatt aactccggtt attcaacttc aatacttttg acgatcagcg aatcgccggt
     8941 atcgacacgc aaccgctcgc cgtgacagag cggacactgc gcatcgtgct gatgaatctc
     9001 caccacctgg ctgcaatccc agcaccaagc ctgggcgggt ttatagacga tatgtaaatc
     9061 gcacccttgc gccaccgttc cgtggcagac aatttcaaaa ctaaaacgga cggcgctctc
     9121 ctcaacgcag gagagcgcgc caatttccag ccacacggcg gtgacgcgct taacatcgtg
     9181 ctgctccgcc tgccgttgga taatttcaac ggcgctctgg caaagagaca actcatgcat
     9241 tttcgccact cctgcgacca aacagcaggg cgcgacgccc tgcgtgtggt acatccggat
     9301 tcgtgactgg cagcgaaagg atcattcgtg cgcagtcatc cgtcaggcgt tggccctctt
     9361 caatcgacat gctgtgcgaa agcggcgaca tcagcgaaca ggagagatat tgcgaaacac
     9421 cgtccagttc gccaacggta aaggtcatag tgccatacgg cagttgcaga ccaatttttt
     9481 cactcacttt gcgcagcggc cagagttgat ccgggccggg gaagataact gcactcagca
     9541 tccacggggt gatcacacac cccgtccact gaccttcgaa cagcgtaaaa tcagaaacat
     9601 acaccggcat tgaaggatgc agaaaagaga gatcgtgcat cgaacgccgg gcaatttctt
     9661 caaacgctgc ctgtacttgc gccttcgggg aggtctggaa acctgctatc tcttcagtca
     9721 tgaatcgcct cccgtgggat ggcttccacg cctgattcgc gcagcgcagc cagaacctgc
     9781 tcaagcgcag gttcaatcat tgcttcaacc gtcggcgtta agccgatgtg tggctccagc
     9841 gattccggga tcacgccgac cagggtcagc tttttcggaa actcaccggt gaagcgcagg
     9901 gccgacaata cgtcggccag gccaagctga tgcggagaga ttttgttggt aaacaacgcc
     9961 ggaacttctt catcccgcag gatcatcatc gttcccggcg tgttcttttt cgacacaatg
    10021 gcatccgcaa taatcagatg atcgcgattt gccatgtcgc caagcagctc cattcccgcc
    10081 gtgccgccat cgaggatctc aacataatcc ggcagaatgt atcgttgctc taacgcttcg
    10141 acaatccgca caccgatggc ttcatcggtc agcaaaatat tgccgacccc taagactaaa
    10201 atacgcatta cagaaccttc actgaaacca cttcgttgcc gtcggcatcc actacgtgta
    10261 ccgcacaggc catgcacggg tcgaaggagt gaatggtacg caccacttcc agcggtttat
    10321 tcggatcagc aaccggtgta cccaccagcg actgctcgta aggaccgacg tcatcattga
    10381 agttacgcgg accagagttc caggttgatg gaacaactgc ctggtagtta ctgataatac
    10441 cgtctttaat aaccatccag tgagagagca taccgcgcgg cgcttcgagg aagccaacac
    10501 ctttgaactc acccgttgcc ggaatgttcg gtttcacaaa ggtggtgtga tcgcctttgc
    10561 cgatattggt gatcagtgca ctgtattgat tttgcaggat atcctgcaat tcgcagcagt
    10621 gaacggtacg accaataata cggcccagcg tggagtgcag ctgcgccact tccagcgtgt
    10681 tgccagtcag tttctgataa atcgcaacga tttcattcag tttgttctgg gtagattcgc
    10741 gacctgccgc cagtttcacc agcatattag ccagcggacc cacttctacc gttttgccgt
    10801 agaaagtcgg tgatttcacc caggaatatt tcccgtcgtc agaccaacca tcataagccg
    10861 gaatggtggt accttcccac ggtgcctgcg gcgcttcgtc tttataccag gagtgcttcg
    10921 cgctttcctg aatgcctttg atcaggtatt catcggaatg agaagtgatc ggacgatacg
    10981 aggacagatc cgcattctca atgtagccgc ccgggaacag gaagctgccg tttttactgt
    11041 cggtcgggaa ttccggcacg ctcaggtagt tcaccgcacc tttaccgcgc gtcagccatt
    11101 ccgggtagaa cgcggcaata actgcggtat caaccttata aacctgttca acaaagtcgc
    11161 tcagcttgtc gatgaaagac ttgatgtaca tcaggcgctc aaggttcagc acgcccaaac
    11221 cgtcgaggtt gatcgggttc gcgacaccac ctaccgccag gttctgaatg tgcggcgttt
    11281 taccgcccag cagcgccacg acgcggttag cgtcacgctg gcactccagc gcctgcaggt
    11341 agtgcgctac cgcaatcagg ttcacttccg gcggcagttt catcgccggg tgacccccgt
    11401 agccattggc gaaaataccc aactgaccgc tggcaaccag atctttgatc ttgttctgaa
    11461 ctttagtgaa ctcttccgga ctgttcagat gccaggtcga aacgcctttc agcatttcgg
    11521 aggctttggt tgggtcagct tgcagtgcag aagtgatgtc cacccagtcc agcgccgaaa
    11581 gctgatagaa atgaacgata tggtcatgcg tggtgtgcgc agccagaatg atgttacgga
    11641 tgtattgcgc gttaaccgga acgtcgatat tcagcgcact ttctgccgca cgaacggaag
    11701 acagcgcgtg agtggtagta catacgccac agatacgttg cacaatcatc catgcatcgc
    11761 gcggatcgcg gtttttcacg atctcttcca tgccgcgcca catggtaccg gaagcccatg
    11821 ctttcgaaac gacgccattt tcgatttcgc aatcgatgcg taaatgcccc tcaatacggg
    11881 ttaccggatc aatagtaatt ctctggctca tgctttgctc gcctcatgac gattatgatc
    11941 gttttgtttt aaaggaggaa gtatcggcag tagacgaatg agtacgatgt aagcgcaaat
    12001 ctcaatagcc acaaaaccaa tagaaatcaa cagttcttcc caggtcggga agtaggcgta
    12061 accgccgccc gggttgaatg ccaccagcga ataggtcaga cgccaggttg cacaacctaa
    12121 cagtgcgctc agtgctgaca ggaacagcat gcgggaatca ttacgcagct tcgccacacg
    12181 cagaacgacc agcgggaaga gcatcagcag gacttcaatc cagaacatca cggagtagaa
    12241 gtcaccggca aacgctaacg acagcttgtc gcgatagata agctcgccaa agcgcagcac
    12301 gatgaaaatc gccagcaaca cactgatggt gttggtcagc ttgacgaaca gactcttttc
    12361 atccggaccg ttgccacgca gacccgcctg caccagcgaa ccttcaaaga tgacaatcga
    12421 gaagcccatg atgaacgccg tcagcagcga gaacagcggc aacatttcat agctctgcca
    12481 caacggatgc accttgtagc ccgccgagat catcagtgac cccattgaag actggtgcat
    12541 ggtcggcaac agcgcgccaa gtgcaatgat gaagaacata accttgttca gacgcttgag
    12601 agaaaccttc cagcccagac gttcaaacag tgccggagca aactccagtg ccatcacgcc
    12661 gatatagatg gtcatacaga ccgccgtctc gaacagtacc gagttcacgt tgaagtgacc
    12721 cggaatgtag aagtacggca ggttccagta gcgacccacg tcgatagtga tcgacaagcc
    12781 acccagtgag taaccaaaca gactcgccaa cagcgccgga cgcaccagcg gatggtattg
    12841 cccacggtta aagacgtata ccgcccacgc cagcgcccag ccgccacagg caaagccggt
    12901 gccaatcagc aggtcaaacg cgatccacac gccccacggg aagccgccgt tcaggtcaga
    12961 gacagagccc agaccgaaca ccagacgctt cacaatcagg agcatacaga tgacgattaa
    13021 cggtccaaaa atcatgaccg gtttactgat gattttgccg cccagcggtt gtggatcatg
    13081 actcatgatc gtctcctccg tcgtgatggt cgtttttggt gttgcgacga accagcacgg
    13141 ttaagcccgc cagcacagcc agtggtagca tcatgccttt atacagggtg tgttgaatat
    13201 tttcggaacg cgcaccggta gaaagatcgt ccagtttcgg caggtcgaga ttttcataag
    13261 gcacacccgt cagtaccaga acctgagtac cgccgccctc tttctcgccg tacagatgcg
    13321 gataatattt cggcaccgta tgcaggtaag tgtcgccaga tttcagcgtc tgacgcggat
    13381 agtggtattc gctgccaggc ttcagcgcca gacgtttttt cgcctccgcc atcagctctt
    13441 cacgcgtacc gaaaatcacc gcgcccgccg ggcacacttc tacgcagcca ggcagaccgc
    13501 ctttatcgag acgttccaca cctttctggt tgcacagctc gcacttatgc agcgcaccaa
    13561 acgggttgtt gtagtcgtac ttcggcacgt tgtacggaca ggcgaccatg cagtaacggc
    13621 aaccggtgca cacgtctttg tcgtaatgga caatgccggt tttcggatct tttttcagtg
    13681 cagagaccgg gcacacagag acacagttcg gatcgacgca gtgcatacac tgtttcttaa
    13741 tgtacgcgta gccgttctcc tcctggtctt tgttgacccc tgtgccgctg gtccacacct
    13801 gaatgatgtt attggtatac ggcgacagtt tgtcgttgtt cgaccaggtc tgttcccctt
    13861 gcgggttacg ttcagggaag ttgatatcct gacacttggt gacgcaagcc tggcagccta
    13921 cgcacaaggt cgagtcgtac aacatcccca gcgatcccgg aattggcggg cggttttcag
    13981 cagccgcatg actgacagac ggcagcgcgc ccgtcagcaa tgccccgcag gaggctgctt
    14041 taataaaatt acgtctgttc acggttattc tccccgtgag tcagcgttat ctttcttttg
    14101 ctgacgaccc agttcacgca ccgccatcac gctgacaccg gcaaccagcc caaccacacc
    14161 gccgagcaaa ccaatagcgc ctgcagagac gttgccgccc tctttagcgt taacatccgg
    14221 tttctgtgaa cgcggagtct gattttcgac gttggcaagc tgatggatgc ctttatggaa
    14281 gccgatacct tcttcgttac agccatagca tgggtgacca atcgctaccg gccacacgcc
    14341 gccaacatcg cagaattgca gcgttgagca gttgccgtaa gtttctggcc ctttacagcc
    14401 gaggtggtac aggcaccagc cttcacggtg gccttcatca ccgaactctt tggcaaaacg
    14461 accagcatcg aagtgcgggc gacgttcgca gtgttcgtga atcagacggc cataggcgaa
    14521 ggtcggacgg tttttgtcat ccagtttcgg cggtttgccg taagtgatga tgtgcgcaac
    14581 ggtcgcgagg aagttgtgcg ggttcggcgg gcagcccgga atattgataa cggttttgcc
    14641 tggcagaact tcttgcaggc tgactgcgcc agttgggtta actccagctg cggcaacacc
    14701 gccccacgca gagcaggaac cgatagcgat aatcgctgct gcgccttctg ccgctttgcg
    14761 gatgtgatcc acaatcggct caccagcaac catgcaataa ataccgttat cttttaatgg
    14821 gatggaacca tccacaacca acacatactg ccctttgtac ttctcaagtg cgttatgttt
    14881 gttctcttcg acctgatgac cgaaggcggc ggaaagcact tcgtgatact ccagagagat
    14941 agtctccagc acgaggtttt ctaccgttgg atgcgttgca cgaagcagag attccgtaca
    15001 accggtgcac tcctgcgcgc caatccagat aactggcgga cgctgcgggt tagtaaccga
    15061 ttcggccatc tctgcagcgg ctttgctact taaccccatg gtggcggcta atgctgcaca
    15121 aagcttcatg aaatcacgac ggttaatgcc gtgagaatgg atgagggtgt tatctccagt
    15181 catttatagt tattccgttg cgaagacctg gcatatattt tgcctcaatc gcaaaatcaa
    15241 taatgcgatc gatgcgccat ttaccacaca tttattatgg ttatcgatat catgatactg
    15301 cggcggggcg ctaaaacgaa gggaagaagc attagtgtaa ttaattaaag caagataata
    15361 cgtatgtttg atcaattttc gaggcgataa ccggccataa aaaagcgcaa cgtagaacag
    15421 gaattattct cacgaaagaa atccattacc atctctttat ccagtccata gcggcgcgtc
    15481 agaatacccg cttcccaggc gctaagctgg cgatctccgg tcttttcgta catccggtgt
    15541 gagtagccta atacaaaacc gcgtttataa tcggcacaga attttgtcac attaaccgca
    15601 ctgtcggcat gcgttgcttt taatcccgcc attaagcctt taccaaaatg gttattcatc
    15661 gttcacctca ttcgctatat atcgccatat atagcgatat tttaggcaaa gaacgacttt
    15721 tgcgcacgct attttttcat gcttgtttaa gacgtaatcc agtaagagat aaacctatca
    15781 ggagagatat ttatcgaacc atttcagtgt cctttgccag gcaagatcgg cggcagattt
    15841 gtcataacgg ggcgtggaat cattatggaa tccgtgatta acccccggat agatatacgc
    15901 ctcataaacc ttattattgg ctttcaacgc cgcctcgtaa gcaggccagc cctcgttgat
    15961 tcgggtgtcc agttccgcga agtggagtag taaaggcgct tcaatcttcg ccacatcggc
    16021 agtgggtgcc tgacgaccat aaaacggcac cgcgcaggcc agttccggat acgcgacagc
    16081 cgccgcgttc gatacgccac cgccatagca aaatccggta atacccactt tgcctgccgc
    16141 ttgtggatag cgttgcataa actcaattgc ggcaaagaaa tcattcatca gtttggttgg
    16201 atcaacctgc tgttgcagct cacgaccttt atcatcattt cccggataac ctccaacgga
    16261 acttaagccg tcaggtgcca gggcgatata ccccgccttc gccactcgcc gtgccacatc
    16321 ttcgatatac ggattcagtc cacgattctc atgcaccacc accacggctg gcgttttgcc
    16381 gctcattttt gcgggtttca ccagataacc ccgtacctcg ccgtgaccat ttggcgaagg
    16441 atacatgatg tactcagcaa caatctccgg gtcggtaaac tctacctgag tcgccagcgc
    16501 ataatttggc ttgagcaaat caaacaacgc taatgccgtc atcccgccca ccgcatactt
    16561 cgccgcaagg ttgaggaact cacgtttcga aattttcccg tgagcgtaat agtcgtagta
    16621 atccaacaac tcttgtggga aatctttggc ggttaaacgc ggcatcgttg cactcctcag
    16681 ttggtgtttt tttaagcaaa gcataagcac gtgtttttgc ccagtttttc gtcactctgt
    16741 gagccagact acgggatacg cgctggcgaa tcgctaaact agaaacattg tttcgaaatt
    16801 gaacggtgga aaggagaggt catggtctgg ttagcgaatc ccgaacgtta cgggcagatg
    16861 caataccgct attgcggaaa aagcggttta cgcctgcccg cgttatcgct cggtttatgg
    16921 cacaatttcg gtcacgttaa cgcgctggaa tcacagcgtg cgatcctgcg taaagcgttt
    16981 gatttgggca ttacgcactt tgatttagcc aacaattacg ggccgcctcc aggaagcgca
    17041 gaagagaact ttggtcgcct gctgcgggag gattttgccg cttatcgcga tgaactgatt
    17101 atctctacca aggctggcta cgatatgtgg cccggccctt acggctctgg cggttcacgt
    17161 aaatacctgc tcgccagcct cgaccaaagc ctgaagcgta tggggcttga gtatgtcgat
    17221 atcttttact ctcatcgcgt cgatgaaaat acgccgatgg aagaaaccgc ctctgcgctg
    17281 gctcatgcgg tacaaagcgg taaggcgctg tatgtcggga tctcctctta ctcgccagag
    17341 cggacgcaaa aaatggtcga gttgctgcac gagtggaaaa ttccgctttt aattcatcaa
    17401 ccttcgtaca atttactgaa ccgctgggtg gataaaagcg gcctgctgga taccctgcaa
    17461 aataacggcg tgggctgtat tgcctttact cctctggctc agggattgct gaccggaaaa
    17521 tatctcaacg gcattccgca agattcacgg atgcatcgtg aagggaataa agttcgtggt
    17581 ctgacgccga aaatgcttac cgaagccaac ctcaacagcc tgcgcttatt gaatgaaatg
    17641 gcacagcagc gtggacaatc aatggcgcaa atggcgttaa gctggttgct gaaagatgat
    17701 cgcgtgacgt cggtattgat tggtgccagc cgcgcggagc aactagagga gaacgtgcag
    17761 gcgctgaata atctgacatt tagcaccgag gagctggcgc agattgatca gcatatcgcc
    17821 gatggcgagc tgaatctgtg gcaggcgtcg tccgataaat gacctgttaa taacgggcgc
    17881 gcgaaccgcg cccgaatcag atcagtgatt atgacgagtc agtcggtcaa gatagcccat
    17941 cacaaatgca gaaagcacaa acgtcagatg gataatgacg taccacatca gtttgttatc
    18001 cgggacattt ttcgcatcca taaagacgcg cagtaagtga atggaagaaa ttgccacaat
    18061 cgacgctgct actttgtttt tcagcgacgt tgcgtccatt ttccccagcc agttcagctt
    18121 ctctttgttc tcggagatat ccagctgtga gacgaaattc tcataaccgg aaaacatcac
    18181 catcaccagc aaaccgccaa ccagcgtcat atccaccagc gacagcaaca cgaggatcaa
    18241 atctgattcc gccatcgaga agatattcgg cagtacgtga ataatctcct ggaagaactt
    18301 cagcgccagg gcaactaacg ccagcgaaag gccaaagtac acgggggcaa gcagccagcg
    18361 agaagcatac attgcatttt caagaaaacg ttccatacat tcctgtcgtg tagttaaaac
    18421 gggcaacagt atatcgcaat tgagtaaaca gcacgcaaca gcaaatcaac atatacgtta
    18481 aacctttgtc gcataattct gaggtttagg gatcttcctg gttcccttat caacgcatcg
    18541 agagcgtcta cccttaaagg acgttgaacc tgaagggaga aaaacgatgt ctcatttaaa
    18601 agacccgacc acgcagtatt acactggtga atatcccaaa cagaaacaac cgacgccagg
    18661 catccaggcg aagatgacac cggtaccgga ttgcggcgag aaaacctatg ttggtagcgg
    18721 tcgcctgaaa gatcgtaaag cactggtgac agggggcgat tccggaatag gtcgcgctgc
    18781 cgccatcgct tacgcgcgtg aaggggctga cgtggcgatc agttatcttc ccgtggaaga
    18841 agaagacgct caggatgtga aaaagatcat tgaagaatgc ggacgcaaag ccgttctgct
    18901 gccaggcgat ttaagcgatg agaaattcgc ccgttcgctg gttcacgaag cgcacaaggc
    18961 gttaggcggg ctggatatta tggcgctggt cgccgggaaa caggttgcca ttccggatat
    19021 tgcagacctc accagcgaac agtttcaaaa gacctttgcc attaacgttt tcgcgctgtt
    19081 ctggctaacc caggaagcga tccccctgct accgaaaggt gcaagtatca tcaccacttc
    19141 gtcaatccag gcataccagc caagtccgca tttactggac tatgcggcta cgaaggcggc
    19201 gattctgaac tacagccgtg gcctggcaaa acaggtcgcg gagaaaggta ttcgggtgaa
    19261 tattgtcgcg ccaggcccta tctggacggc actgcaaatt tccggcggac aaacgcagga
    19321 taagatcccg cagtttggtc agaaaacgcc gatgaagcgt gcggggcaac cggcggaact
    19381 ggcccctgta tatgtttatc tggcaagtca ggagtcgagc tacgtcaccg cagaagtgca
    19441 cggcgtgtgc ggcggcgagc atttaggtta aaaaaatgcc cggttgtgaa aagcaaccgg
    19501 gcatcattgt gaattcactc ttacctgatg tggcgaaaac gccttatccg gcctacaaac
    19561 gcacgaagat tcaacatgtt gcaatagatt ttgtaggcct gatgagcgta gcgcatcagg
    19621 caattctact ttacttcgct ttggcggttt cttcgccgac cagacctatc ttcaggtaac
    19681 ccgcctgatg cagcgtatcc attaccttca tcaacgtctc gtaatcgacg gttttgtccg
    19741 ctcggaagaa gatggtggtg tctttcttgc cttcggttaa cgcattcaac gccgtaatca
    19801 ttgtttcatc ggtgaccgga tcgttaccga taaacatcga gttgtctgcc ttcaccgaca
    19861 gataaaccgg tttttccggg cgcggctgcg gcgtgctggt agaagcaggc aagttcacct
    19921 tcacatctac cgtcgctaac ggtgccgcca ccataaagat aatcagcaga accaacatca
    19981 cgtcgataaa cggcgtcacg ttgatatcat gcatttcgcc gttatcgtcg aggttttcgt
    20041 taagatgcat tgccatcgga cattatcctg cgcgtaattt ttgtgcgaca cgaaccggat
    20101 gcgcagcggc gctggcttcc agatccaggt cacggctttg cagcaacaat acctgcgctg
    20161 caacatcacc cagcatcgct ttaaagccgc caatctggcg tgcaaatacg ttatagataa
    20221 cgaccgcagg aatcgctgcc acgaggccga ttgccgttgc taacagagct tctgcgatac
    20281 ccggcgcaac gactgccagg ttagtggtct gcgtttgcgc gataccaata aagctgttca
    20341 tgatgcccca gaccgtacca aacagaccaa cgaacggaga aatcgcgccg atggttgcca
    20401 gatagccgtt accgcggccc atttgacgac ccactgcggc gacccgacgc tccagacgga
    20461 agctggtacg ttctttaata ccttcgttat cgtcgctgcc ttctgacagt tccagctcat
    20521 tctgcgcttc attgagcaaa tgcaggctta agcttttgct accaaaatca gcggcgatat
    20581 cgttggcctg gtttaaggaa cgcgcttcag ccagcagttg ctgctcgcgc ttaaggcgac
    20641 gcttctgatt gaagaactct acgctcttac tgaagaagat tgcccaagtg actacggagg
    20701 ccaaaataag cccaatcatc acgcacttaa cgacaatatc ggcgtgctga tacatacccc
    20761 agacggaaag gtccgtctgc atcaaattat tacccactgt gtatctccag gacgcaagtc
    20821 acaaaatctg cgcataataa tatcaaaatg acgtcgaatt gatagtcgtt ctcattacta
    20881 tttgcatact gccgtacctt tgctttcttt tccttgcgtt tacgcagtaa aaaagtcacc
    20941 agcacgccat ttgcgaaaat tttctgcttt atgccaattc ttcaggatgc gcccgcgaat
    21001 attcatgcta gtttagacat ccagacgtat aaaaacagga atcccgagat ggcggacaaa
    21061 aagcttgata ctcaactggt gaatgcagga cgcagcaaaa aatacactct cggcgcggta
    21121 aatagcgtga ttcagcgcgc ttcttcgctg gtctttgaca gtgtggaagc caaaaagcac
    21181 gcgacgcgca atcgcgccaa tggcgagttg ttctatggac ggcgcggaac gttaacccat
    21241 ttctccttac aacaagcgat gtgtgaactg gaaggtggcg caggctgcgt gctatttccc
    21301 tgcggggcgg cagcggttgc taattccatt cttgcttttg tcgaacaggg cgatcatgtg
    21361 ttgatgacca acaccgccta tgaaccgagt caggatttct gtagcaaaat cctcagcaaa
    21421 ctgggcgtaa cgacatcgtg gtttgatccg ctgattggtg ccgatatcgt taagcatctg
    21481 cagccaaaca ctaaaatcgt gtttctggaa tcgccaggct ccatcaccat ggaagtccac
    21541 gacgttccgg cgattgttgc cgccgtacgc agtgtggtgc cggatgccat cattatgatc
    21601 gacaacacct gggcagccgg tgtgctgttt aaggcgctgg attttggcat cgatgtttct
    21661 attcaagccg ccaccaaata tctggttggg cattcagatg cgatgattgg cactgccgtg
    21721 tgcaatgccc gttgctggga gcagctacgg gaaaacgcct atctgatggg ccagatggtc
    21781 gatgccgata ccgcctatat aaccagccgt ggcctgcgca cattaggtgt gcgtttgcgt
    21841 caacatcatg aaagcagtct gaaagtggct gaatggctgg cagaacatcc gcaagtagcg
    21901 cgagttaacc accctgctct gcctggcagt aaaggccacg aattctggaa acgagacttt
    21961 acaggcagca gcgggctatt ttcctttgtg cttaagaaaa aactcagtaa tgaagagctg
    22021 gcgaactatc tggataactt cagtttattc agcatggcct actcgtgggg cgggtatgaa
    22081 tcgttgatcc tggcgaatca accagaacat atcgccgcca ttcgcccaca aggcgagatc
    22141 gattttagcg ggaccttgat tcgcctgcat attggtctgg aagatgtcga cgatctgatt
    22201 gccgatctgg acgccggttt tgcgcgaatt gtataacatt gccacttttg gacaattttg
    22261 cagacattta attgtgaaaa gtcttaaatt gttgcgtccg ggatcaaggc gtcccggacg
    22321 aatcaggagt acaataggca gataaaggct taaacgctgt tccacaggaa agtccatggc
    22381 tgttattcaa gatatcatcg ctgcgctctg gcaacacgac tttgccgcgc tggcggatcc
    22441 tcatattgtt agcgttgttt actttgtcat gtttgccacg ctgtttttag aaaacggcct
    22501 gctgcccgcc tcatttttgc caggcgacag cttgttgata ctggcaggcg cattgattgc
    22561 ccaaggggtt atggattttc tgcctacgat tgcgattctg accgccgcag caagtctggg
    22621 ctgctggcta agttatattc aggggcgctg gttagggaat accaaaacgg tgaaaggctg
    22681 gctggcacag cttcctgcta aatatcacca gcgcgccacc tgcatgtttg accgccacgg
    22741 tctgctggcg ctgctggctg gacgttttct tgcatttgtc cgtacgctgc tgccaaccat
    22801 ggcgggaatt tccggtctgc caaaccgccg cttccagttt ttcaactggt taagtggatt
    22861 gctgtgggtc agcgtggtaa ccagttttgg ctatgcctta agtatgattc cgttcgttaa
    22921 acgccatgaa gatcaggtaa tgacgttcct gatgatcctg ccaattgcct tgttaaccgc
    22981 tggcttgtta ggcacgctgt ttgtagtgat taaaaaaaaa tactgtaacg cctgacgatt
    23041 ttccccgttc ccggctgctg taccgggaac gtatttaatt cccctgcatc gcccgcattc
    23101 ttgccgcatc ttcccccggc gtcacaccga agtaacgttt aaactcacgg ctaaattgca
    23161 atgcgctttc atagtcgacg cgcatcgctg ctgcgctggc cttcatgccg tcatggatga
    23221 tcatcatccg cgccttatgc agacggtaat tcttcaaata ctgcaacggc gaggtgctgg
    23281 tgacagactt aaaattatga tggaacgccg atacgctcat gttggcttct gccgccagtt
    23341 gctcgacgct caggttttcg gtgtatttat tctcaatccg tttcagcacg cggctaatca
    23401 gactgaagtg agtctggcga ctgaccagcg ccagtaacgc gccgccgcaa ggtccggtca
    23461 gcacgtagta cagaatttcg cggatgatct gtttgccgag aatacgcgca tccagtggtc
    23521 gctccatcac gtcgagtaac cgctccgccg cgcataaaat ctcttctgat aacgtggcgg
    23581 agttaatccc gctggctgcc atcgacggct ggaaatgctc atcttcgcca atgtccatca
    23641 acagttcctg taactgcaaa atatcgacat tgagacgcaa tcctgccagc ggcacctctg
    23701 acgtggcata ggtttcgcac tcaaacggca acggcaccgt cagcagcagg tattcattgg
    23761 catcataacg aaacacgcgt tcattgatat aaccgatttt atgcccggaa aagagaatta
    23821 tgatgccagg ctcgtacatc accggtgtac gtgcgaaagg cgtctcgcca tacaacaaac
    23881 gcacatcggg caacagttct gacaaactat tttctttatt tttcagttta ttaactttat
    23941 ccgccagcaa gcggcaaatc tcttcacgtt tcatatcgcg taatttctta ggaataatgc
    24001 ggcaatttga ttgtgcgcaa ttttgtagca tttctccagc actctggaga aataggcaag
    24061 acattggcag aaatgagcat tgagagccag ggcgctggcg atcacaatga aaaacatcag
    24121 gcagatcgtt ctctgccctc atattggccc agcaaaggga gcaagtaatg aacaacttta
    24181 atctgcacac cccaacccgc attctgtttg gtaaaggcgc aatcgctggt ttacgcgaac
    24241 aaattcctca cgatgctcgc gtattgatta cctacggcgg cggcagcgtg aaaaaaatcg
    24301 gcgttctcga tcaagttctg gatgccctga aaggcatgga cgtgctggaa tttggcggta
    24361 ttgagccaaa cccggcttat gaaacgctga tgaacgccgt gaaactggtt cgcgaacaga
    24421 aagtgacttt cctgctggcg gttggcggcg gttctgtact ggacggcacc aaatttatcg
    24481 ccgcagcggc taactatccg gaaaatatcg atccgtggca cattctgcaa acgggcggta
    24541 aagagattaa aagcgccatc ccgatgggct gtgtgctgac gctgccagca accggttcag
    24601 aatccaacgc aggcgcggtg atctcccgta aaaccacagg cgacaagcag gcgttccatt
    24661 ctgcccatgt tcagccggta tttgccgtgc tcgatccggt ttatacctac accctgccgc
    24721 cgcgtcaggt ggctaacggc gtagtggacg cctttgtaca caccgtggaa cagtatgtta
    24781 ccaaaccggt tgatgccaaa attcaggacc gtttcgcaga aggcattttg ctgacgctaa
    24841 tcgaagatgg tccgaaagcc ctgaaagagc cagaaaacta cgatgtgcgc gccaacgtca
    24901 tgtgggcggc gactcaggcg ctgaacggtt tgattggcgc tggcgtaccg caggactggg
    24961 caacgcatat gctgggccac gaactgactg cgatgcacgg tctggatcac gcgcaaacac
    25021 tggctatcgt cctgcctgca ctgtggaatg aaaaacgcga gaccaagcgc gctaagctgc
    25081 tgcaatatgc tgaacgcgtc tggaacatca ctgaaggttc cgacgatgag cgtattgacg
    25141 ccgcgattgc cgcaacccgc aatttctttg agcaattagg cgtgccgacc cacctctccg
    25201 actacggtct ggacggcagc tccatcccgg ctttgctgaa aaaactggaa gagcacggca
    25261 tgacccaact gggcgaaaat catgacatta cgttggatgt cagccgccgt atatacgaag
    25321 ccgcccgcta agctttttac gcctcaaact ttcgttttcg ggcatttcgt ccagacttaa
    25381 gttcacaaca cctcaccgga gcctgctccg gtgagttcat ataaaggagg aacgtatggc
    25441 taatccaacc gttattaagc tacaggatgg caatgtcatg ccccagctgg gactgggcgt
    25501 ctggcaagca agtaatgagg aagtaatcac cgccattcaa aaagcgttag aagtgggtta
    25561 tcgctcgatt gataccgccg cggcctacaa gaacgaagaa ggtgtcggca aagccctgaa
    25621 aaatgcctca gtcaacagag aagaactgtt catcaccact aagctgtgga acgacgacca
    25681 caagcgcccc cgcgaagccc tgctcgacag cctgaaaaaa ctccagcttg attatatcga
    25741 cctctactta atgcactggc ccgttcccgc tatcgaccat tatgtcgaag catggaaagg
    25801 catgatcgaa ttgcaaaaag agggattaat caaaagcatc ggcgtgtgca acttccagat
    25861 ccatcacctg caacgcctga ttgatgaaac tggcgtgacg cctgtgataa accagatcga
    25921 acttcatccg ctgatgcaac aacgccagct acacgcctgg aacgcgacac acaaaatcca
    25981 gaccgaatcc tggagcccat tagcgcaagg agggaaaggc gttttcgatc agaaagtcat
    26041 tcgcgatctg gcagataaat acggcaaagc cccggcgcag attgttatcc gctggcatct
    26101 ggatagcggc ctggtggtga tcccgaaatc ggtcacacct tcacgtattg ccgaaaactt
    26161 tgatgtctgg gatttccgtc tcgacaaaga cgaactcggc gaaattgcaa aactcgatca
    26221 gggcaagcgt ctcggtcccg atcctgacca gttcggcggc taacatgcaa attctcccgg
    26281 tgacggtaat gttccgctac cggacttttc agaaatcatt tattcccctc gcgtcccgcc
    26341 cgttgttact cttccttgtt caggaatgcc aaatataagg acatcatcat gcagagccgg
    26401 aagctcttaa aagaacaact catctatatc cgggataaac gcaacggaga ggtgaaaaac
    26461 agatgaaaat aatacttctg tttttagcag ccctggcaag ttttaccgta cacgcgcagc
    26521 ctccttcaca gaccgtagaa caaacagtcc ggcagattta tcagaactat aaatcagatg
    26581 ccagtacacc ttatttcggt gaaaccggag agcgggcgat aacttcagcg cgtattcaac
    26641 aggcgcttac cctgaacgac aatcttacgc tgccgggcaa tattggctgg ctggattatg
    26701 atccggtttg tgattgtcag gattttggcg atctggtgct agaaagcgtt gcgataaccc
    26761 aaactgacgc cgatcatgcc gatgccgttg tgcgctttcg tatctttaaa gatgataaag
    26821 aaaagaccac gcagacactg aaaatggtgg cggaaaatgg tcgttgggtc attgacgata
    26881 ttgtcagcaa tcatggcagc gtcttacaag cagttaatag cgagaatgaa aaaacgctgg
    26941 ccgctttagc ttcgttgcaa aaagaacagc cggaagcctt tgttgccgaa ctctttgaac
    27001 atattgctga ttatagctgg ccgtggacgt gggtggtttc cgactcttac cgccaggcgg
    27061 ttaatgcctt ctataaaacc accttcaaga cggccaataa tcccgatgaa gatatgcaaa
    27121 tagaacggca atttatttac gacaatccga tctgttttgg cgaagagtcg ctattttcac
    27181 gcgttgatga aattcgagtc ctggagaaaa ccgccgattc cgcccgcatt catgttcgtt
    27241 ttacgctgac caatggcaac aacgaagagc aagaactggt tttacagcgg cgcgaaggca
    27301 agtgggaaat cgctgatttt atccgcccga acagcggcag cctacttaag cagattgagg
    27361 caaaaaccgc cgccagatta aagcaatgag ctgaattaaa taacaattag ccggaacaat
    27421 aaataaaagg gaacactata tgaaaacgat tttcaccgtg ggagctgttg ttctggcaac
    27481 ctgcttgctc agtggctgcg tcaatgagca aaaggtcaat caactggcga gcaatgtaca
    27541 aacattaaat gccaaaatcg cccggcttga gcaggatata aaagcactac gcccacaaat
    27601 ctatgctgcc aaatccgaag ctaacagagc caatacgcgt cttgatgctc aggactattt
    27661 tgattgcctg cgctgcttgc gtatgtacgc agaatgataa aaaaatcccc ggcagcatgt
    27721 cagttgccgg ggattttttt taacgtccaa ccgccgcttt agggcgtttc ttcgcaccag
    27781 cattcaccgg acgagattgc gtagacgacg ctttttttgc cgtagcaggc gtctgacgct
    27841 gggtcgccat cggcgtatgt ttagtcaacg ccggacgggt attgcggttc tggcgacgag
    27901 cttcacgcat ctcttcaatg gttggcgcag gcactaagca atcgcgacgg ctgccaatca
    27961 gatgcttttt gcccatcgct tccagcgccg ggcggattaa cggccagttt gccggatcgt
    28021 ggtaacgcaa caacgcttta tgcaaacgac gctgtttgtc gcccttcggt acgaagacgt
    28081 cttcgctctt ataaccaatc ttcgccagcg gttttttccc ggtgtaatac atggtggttg
    28141 agttcgccag cggcgatggg tagaagttct gtacctggtc gagacggaaa cgatgctttt
    28201 tcagccacag cgccaaattc accatatctt catcacgcgt accggggtgc gcagagatga
    28261 aatacgggat cagatactgc tctttacctg cctgtttcga gtaggtatcg aacagctctt
    28321 taaagcggtc atagctgccc atgcccggct tcatcatctt cgacaacggc ccttcttcgg
    28381 tatgttccgg agcaatcttc agataaccgc cgacgtgatg ggtcgccaac tctttgatat
    28441 agcgcggatc ttccacggcg atgtcataac gtacgcctga ggcgatgagg atttttttaa
    28501 tgcctttcag atcacgcgca cggcgataga ggttgatcgt cggttcgtgg ttagtgtcca
    28561 tgtgcggaca aatatccgga taaacgcacg acaaacggcg acaagtttgt tcagcgcgtg
    28621 gcgatttgca gcgcaacata tacatgttgg cagttggccc accgagatcg gaaatcacgc
    28681 ccgtaaaacc tggaacggtg tcgcggatcg cttcgatctc attaatgatc gaatcttccg
    28741 aacggctctg aataatgcgc ccttcgtgct cggtaataga acagaaagaa cagccgccaa
    28801 agcagccgcg cataatgttg accgaaaaac ggatcatttc gtaagccgga atacgggcat
    28861 tgccataggc cggatgtggc acgcgcttgt acggcagcgc aaaaacgctg tccatctctt
    28921 cggtagaaag cgggatagca ggcgggttga tccacacata gcggtcaccg tgtttttgca
    28981 tcaatgcgcg gacgcagcct gggttggttt cgtggtgcag aatacgcgaa gcatgggcgt
    29041 acagcacttt atcgcccttc actttctcga aagaaggcag caacacgtag gttttttccc
    29101 acggtttcgg gcgcggtggc tgcacggtta cggctttggc ttcctgcttt ttcggtgcca
    29161 ccggtttgtt atccgcgcac ggcaaatctt caccatacgg atgcgggatt gggtcgattt
    29221 ttccaggggt atcaagacgg gtggaatcca cgccgctcca gccaggcagc gcctctttca
    29281 cgataatcgc ggtattacgc acatcgcgga tttcactaat cggctcgccc atcgccagac
    29341 ggtgcgccac ctccaccagc ggacgctcac cgttaccaaa catcagcatg tcggctttcg
    29401 aatccaccag cacggaacgg cgcacggtat cggaccagta atcataatgt gcggtacggc
    29461 gcagactagc ctcaataccg ccgaggatca ccggtacatc tttccacgcc tctttacaac
    29521 gctgggtata aaccagtgtg gcgcgatccg ggcgcttacc cgcgacgtta tccggcgtgt
    29581 aggcatcgtc atgacgtaaa cggcgatcgg cggtataacg gttgatcatc gaatccatgt
    29641 tgccagcagt aacaccgaaa aacagattcg gtttacccag acgcataaag tcgtctttgc
    29701 tgctccagtc cggctgggcg atgatcccga cgcgaaagcc ctgtgcttcc agcatacgac
    29761 cgcaaatcgc catcccgaag cttgggtgat cgacatacgc gtcgccagta accaaaatga
    29821 tgtcgcagct atcccagcca agttgatcca tctcttcacg agacatcggc aaaaacggtg
    29881 ccggtccaaa acaggcggcc cagtactgcg gccaggagaa caggtcgcga tccggttgga
    29941 tcagggagat agagctcatt ttgcttccag aaatgataaa aaaataatca aaggccgggg
    30001 attataagcc ggaacgaaag agaaatcgaa aagtattcca tactcgccct cctcgggcga
    30061 gtatgaagat tacggtaccg gattgactaa cagttgccca atcgagccac ggtccgccat
    30121 ttccagcgtc tgactgttga agtagaacgg gaagtgcgcc caggaaggct gaccgaaata
    30181 aacaagcagc tccacttgtc cgtcaaccca aacggtatct ttccagcctc tgtcttccgg
    30241 gaacggcatc gcaccattca cgttacggat ctggaacatc acgccttcaa tatggaacgc
    30301 ttgcggctcg tccgcgcgta ccgtccagcg ttcccacgtt ccttgctgcg cggtgacatc
    30361 aatacggttg acgtcccaca gctgtccatt aatacccggg tcatcaccca gactgatatc
    30421 gcgactgcga atcggcgaac cggccatgat ttcagttggc agcaagcgca tcggaagact
    30481 gtctgtgacc agcggcagaa ggccggttgg gcgcagcgtt agcaccaggg tagaaaccag
    30541 aatactggat ggctcaaaga agccgcgaat acgatcaaca atgctcgccg cttcgccaca
    30601 ggtgatcgac acttcatcgc cgttgctcat atccaccaga atctcgcggc gctcgcccgg
    30661 tgccagcgaa agttgcttca ccgatacagg agcaggcagg aatccctgat cgccagaaat
    30721 cacatgtaac gggcgaccat cgctcatctg tagttgatag cgacgagagt tcgacgcgtt
    30781 cagcagtcgc aagcgcaccc agccacgcga gacttcaacg tacgggcttt gtacaccgtt
    30841 aaccagcagc gtatcaccaa caaagccgcc gcttcccggt tcgttgtatt ctggcgtacc
    30901 aaagttatcc agccgtttat cctggataat gaccggaaaa tcatccacat cataatggtt
    30961 ggggataggc agcgacttgc tgacttcatc ttccaccagc cacattccgg caaggccgtt
    31021 atagacctgc tgagccgtgc ggttgggagt attggcgtga taccacagag tagctgcgtt
    31081 ctggcgaata ggcagtacgg gtgcccagtc agcgtttggc gacatcatcc gtgccggacc
    31141 gcccatcagc gggccaggta cctgtagccc ggccaccgtc attgagacat tttctgtcag
    31201 gcggttgctg taaataagct taacatcgtc gcccttccag acgcggatag tcggccccag
    31261 gtaacgacca ttgattcccc agaccgacgc gcgtgtccct ggcgtaaatg accagtgcgc
    31321 acgttgtaca gtcataaaca gcggttgccc acggcgagat tcaagtagcg gcggaacggg
    31381 tagcggttgc tgttgcccgg ctgcgctggc cttcagggga acagcgcctg cacaaagtgc
    31441 aatccccgat gcctgaatga actgacgccg actgagtgac atatttgctc catgtaaaac
    31501 tggctaaagt aataccgtgc aggaaagtgt tccccgcaaa aatacagatt gccgacttaa
    31561 acttttccgg cggcttcgcg ttctgcgact tctttatcga gctcggcgat tttttgttcc
    31621 attatcgaac gacaatgggc agccagctca cgaacctgat ctttgccata ctgactgacg
    31681 tcaattggcg gcagcatttc gacaatcacc agaccgttgt gcagtcgatt aagattaatc
    31741 ttattcgaag ttgtagagac gcacacggga ataatcggga cgcccgccgc aattgccgcg
    31801 tgaaatgctc cagtcttgaa cggtagcagg ccgcgaccac ggctgcgggt tccttccggg
    31861 aacatccaga tggaaatacg gcgttttttg aaatgattca cgacttccgc aatggtgccg
    31921 tgagctttag tgcgattgtt tctgtcgatc aataagttgc cggttaacca gtacaactgc
    31981 ccgaagaagg ggatccacag caagctcttt ttacctaccg tcaccgtcgg cggttgcaca
    32041 atgttcgatg ctgtcaccat gtcatagttg ttctggtggt tagcgatata gatagcattg
    32101 ccgtagcttt cagcatctgc aggtttgcgg cactcaactt tcaggccgaa cagcggcgca
    32161 agacggccaa acatatgccc aaaggtggcc acatgtttcg ggttacgcgg gctgaaaagg
    32221 cagtaaatgg agccgaatac acagactaag atgctgtaaa tcacggtaat aataagacga
    32281 aagatatata gcataacacc ctctgaaggt ctgagagact ggcattgtac gtcacctgaa
    32341 atcaggttag ggcattgtta aaagccgctc tctggaaaag cgtattgtta atcgcaacgc
    32401 atgaataaac aacggtttca agggaatttt tatcgtttcc gggggttgaa tacgctgccg
    32461 gatgacgact taacgtttca tccggcgttc cttgcaagcg ggaggaaaca ggccctcccc
    32521 ggcatattac tcttcgctat caccgttgct ggcacggcga ggagagtcga tctcaacacg
    32581 atcgatacgc tgcaaaccgc gcatcaacgt accgcggcgt ccacgttcac cagtgacttt
    32641 ctgtaactct tccgggcgca gtttaatttt gcgtttccca acatgaatgg tcagcgtgct
    32701 ttgcggcggc agaacgtaca attgcgccag accatcttct ccacgcgcgg cttctgccga
    32761 tggaatgttg ataatcttgt tgcctttgcc cttcgacagc tgcggcagat cgcttaccgg
    32821 gaacatcaac atacggcctg cctgagtgat tgccagcagc atatcggaag catcttcaat
    32881 caccaccggc ggcataacat gggcattttc cggtaaggtg atcaaagcct tacctgcacg
    32941 gttacgcgcc accagatcgt taaaggtgca gacgaaaccg taacccgcat cggaagccat
    33001 cagcagtttc tgatcgtcgc tttccatcag catatggtca acggtcgccc caggcggcaa
    33061 cgttaatttg ccggtgagtg gttcgccctg accacgcgcc gacggcagcg taatcgggtc
    33121 gatggcatag ctacgaccgg tggaatcaac aaacactacc gattggttgc tcttaccttt
    33181 caccgccgct ttgaagctat cacccgcttt ataattcagg cccggcgcgt cgatatcatg
    33241 gcctttagcg ctgcgtaccc agcccatctg cgacagcaca atggtgacag gttcagacgg
    33301 cagcatgtcg tgctcgctca tcgctttcgc ttcttcgcgt tcctgcaacg gcgaacgacg
    33361 atcgtcaccg taggcttgcg cgtctgcctg cagttctttc ttcagcaggt tattcatttt
    33421 acgctcggaa gccaaaatgc cctgcaactg gtcgcgctct ttttccagct cactctgctc
    33481 accgcgaatc ttcatctctt ccagtttggc aagatgacgc agtttcagtt cgaggatcgc
    33541 ttccgcctgg gtttccgtaa ggccaaaccg cgacatcagc gccggtttcg gttcatcttc
    33601 gttacgaatg atctcaatca cttcgtcgat gttgagaaac gccaccagca aaccttccag
    33661 gatatgcagg cgcttgagga ctttctccag acgatagttc agtcggcggc gcacggtatc
    33721 gcgacggaac accagccatt cggagaggat ttccagcagg tttttcaccg ccggacgacc
    33781 atccagaccg atcatattga ggttaatgcg atagctcttt tccagatcgg tggtagcgaa
    33841 gaggtggttc atcacctgat ccatatccac gcggttggaa cgcggcacaa tcaccagacg
    33901 ggtcgggttc tcgtggtcag attcatcgcg cagatcgtca accatcggca gctttttgtt
    33961 gcgcatttgc gcagcaattt gctccagtac acgcgcacct gaaacctgat gcggtaatgc
    34021 gctgataacc accgcgccat cttctttctt ccacaccgcg cgcatacgca ctgaaccacg
    34081 tccgttctcg tagattttac ggatctcggc gcgcgaagtg ataatttccg cttcagtcgg
    34141 ataatccggc ccctgcacga tatccagcag ctgatcgagc gtggttttcg gctggtcgat
    34201 taatgcgatt gccgcctgag ctacttcacg caggttatgc ggtggaatat cggtcgccat
    34261 gccgacggca ataccggtgg tgccgttaag caaaatgttt ggcagacgag caggcagcat
    34321 tttcggctcc tgcaaagtgc cgtcgaagtt tggcacccag tcagccgtcc cctgccccag
    34381 ctcgctcaat agcagctcgg aatatttcga caaccgggat tcggtgtaac gcattgccgc
    34441 gaacgatttc ggatcgtccg gcgcgcccca gttcccctga ccatcaacca gcggataacg
    34501 gtaagagaac ggctgcgcca tcaggaccat cgcttcataa caggcgctat cgccgtgcgg
    34561 atggtattta cccagtacgt caccgacggt acgggccgat tttttaaatt tggcgctggc
    34621 attcaggccc agttcagaca tcgcatacac aatgcggcgc tgaacaggtt tcagaccatc
    34681 accaataaac ggcaacgcac ggtccatgat cacgtacatg gagtagttta agtaggcgtt
    34741 ttccgtaaat tcatgtagcg caaggcgctc tgccatatcg ctcattaatt ctgattcctc
    34801 aactgattcg ccagccttca aaggggcaat attgccggag atactacatt atctgccgcg
    34861 tcgagtcaca ggagatcagc gtgaatagcg ccgccggacg tgctccgacg gcgataaagg
    34921 catcaatgtg ccttgatata caactctttg ctgtagactt gatcggcata gagggcagca
    34981 caaggttgcg cccatgccac accactcggc atgggggtcc gctccgggcg attcgagaag
    35041 ttgaggttat gcgagtccag ggaagcctaa agcatcaaag tagtcgactg taagtatatg
    35101 gttcagccca gtttagctgt gtctccacca gtgccccgcg cctcctgcca taagtgcggc
    35161 ttgctcatgg gtacccccga gacggcttac ggcgcaccgg ataataaatt tcgcgtatac
    35221 atggtattcc ttaccggaga aattataagc ccaacaacaa tacgcctgtg gcattgttcg
    35281 ctcaaactta tcgcgagtga tttggctcac actccggtaa agattcccca tcacgctaac
    35341 ggcacataaa catccgtttc cagtcgctgc tccgtcaccc cttcggcaag attggtgtag
    35401 tgaaacagaa tcggtgcttt acgcattttc tcgccgcttg caggcagcca gtggcgaata
    35461 atgccccata tcgtgtgtga aatatcgtcc agctcgccaa cgtggcgggc cacggcataa
    35521 cgtccaccgg taagttcacc attgctcaca ccataacgat tatcgggaat cggttcgcta
    35581 acgctgccgc agatatcaaa gcgaaacgct tccggtgcgg tggtggcagg gtcgtcccag
    35641 gcgacgccaa aagtctggct ttggttaaca ggagaaagcc ccgtctcttt acgccacatg
    35701 ataaatttcg ccacactgta gttgagcaac tcagggctac agcggtgcgg caacatcgcc
    35761 acagggattg atgggaaatc gataatgttt acatccagtg tcaggtttgt cattttgcct
    35821 ccctgcgaaa ttattcccgg gaacgcgtcc cgggagcggt aacaattacg gatttacttt
    35881 gcggatctgt ttgacgtcaa tttcaacaga attccagtct ttatcgactt caccctgaat
    35941 ctcaaccgta tctttcggcg tcaccgtcac accgttccag cgtttgtggt cgatatcaac
    36001 attgatagta ccgctggcat ctttaaacac gtagagatcg tcagagatgc gttcaacgat
    36061 attgccgcgc agggttaccc aggtgtcgtc acgcagggat tttgcgcttt ctacagtcgt
    36121 tacgctgccg ttcggcccct ggaatcctcc ggcctgactt tgcgttgccg atgggccaga
    36181 aaaaccgccc tgctctgctg ccatcaccgg tgcactgcac agggccatta ctgcgattac
    36241 tgctgcgaat tttttcatgt ttattactcc ctttaatgtc tgtttccgag catttaacaa
    36301 gatagtcctt aacaacttct taagggaaaa aaataaaatt taatgctgta cagagcgcgt
    36361 tacaacacgg tttactggca gcaaatacgg ttatcgcagg gatgaaaaaa tgcgaatttt
    36421 actgatagaa gatgacatgc tgattggcga cggcatcaaa actggcctta gtaaaatggg
    36481 ttttagtgtc gactggttta cacaaggtcg tcagggaaaa gaggcgcttt atagcgcacc
    36541 ttatgatgcg gtgatcctgg atttaacctt accaggcatg gatggtcgcg atattttgcg
    36601 cgaatggcga gagaaaggtc agcgtgagcc ggtactgatc ctgaccgcgc gtgatgcgtt
    36661 agcggaacgt gtagaagggc tgcgtctggg agctgacgat tatctgtgta aaccttttgc
    36721 gttgatagaa gtcgccgcca ggctggaggc tctgatgcgc cgaaccaacg gccaggccag
    36781 caacgagctg cgccacggta acgtcatgct cgaccccggc aaacgtatcg ccacgctggc
    36841 tggcgaaccc ttaacactga aaccaaaaga atttgccctg ctggaattac tgatgcgtaa
    36901 cgctggtcgg gtactgccgc gcaaactgat tgaagagaaa ctgtatacct gggacgaaga
    36961 ggtcaccagt aatgccgttg aagtgcatgt gcatcatctg cgacgcaaac tcggtagtga
    37021 ttttattcgt accgtgcatg gtattggcta cacattaggt gagaaatgaa atttacccaa
    37081 cgtcttagtc tgcgcgtcag gctgacgcta atctttttaa ttctggcctc ggtgacctgg
    37141 ctgctttcca gctttgtcgc ctggaaacaa acaacggata acgtcgatga attgttcgac
    37201 acccaactga tgctgtttgc caagcggtta agtacgctcg atctcaacga aatcaacgcg
    37261 gcggatcgca tggcacagac gccaaataaa ttaaaacacg gtcatgttga tgacgatgcg
    37321 ctgacctttg ccatctttac ccacgacggc agaatggtcc ttaatgatgg cgataacgga
    37381 gaagatattc cctatagcta tcaacgggaa ggttttgctg acgggcaact ggtcggtgaa
    37441 gacgatcctt ggcgttttgt ctggatgacc tcacctgatg gcaaatatcg catcgttgtt
    37501 ggccaggaat gggaataccg tgaagacatg gcgctggcga ttgttgccgg gcaattgatc
    37561 ccgtggctgg tcgcactgcc gattatgtta atcatcatga tggtactact gggtcgtgaa
    37621 ctcgcgccgc tgaacaaact ggcgctggca ctacgtatgc gtgaccctga ctcggaaaaa
    37681 ccactaaacg cgactggcgt acccagcgaa gtgcgaccac tggttgagtc gctaaatcaa
    37741 ctgttcgccc gcacacatgc gatgatggtt cgagaacgac gctttacctc cgatgcagct
    37801 cacgaactac gtagcccgtt aacagcgctg aaagtgcaaa ccgaagttgc gcagctctct
    37861 gacgatgatc cgcaggcgcg gaaaaaagca ctgctccaat tacattccgg gatcgatcgc
    37921 gctactcgtc tggttgatca actgctcacg ctatcgcggc tggactcact ggataacctt
    37981 caggacgtcg cggagatccc gcttgaagat ctcctgcaat cgtcggtgat ggatatttac
    38041 cacacggcgc agcaggcgaa aattgacgtg cgactgacac tcaatgccca cagcatcaaa
    38101 cgcactggac aaccgctatt gctaagtttg ttggtgcgaa accttctgga taacgccgtg
    38161 cgctacagtc cacagggcag cgtggtggac gtcacgctga atgccgataa tttcatcgtg
    38221 agggataacg gccccggtgt gacaccagag gcactggcgc gaattggcga acgcttctat
    38281 cgcccccccg gacaaaccgc taccggcagt ggacttgggc tatcgattgt ccagcgaatc
    38341 gccaaattgc atggcatgaa tgttgaattt gggaatgcgg aacaaggtgg atttgaggcg
    38401 aaggtgagct ggtaagggta agacttttgc taaattcgca aaagactttg cacattttgc
    38461 taattttacc gtaccgctct gtgacgtact atagtcgaca aacgtctcac cttgaggtta
    38521 aaaaatgagc aacaccctga ttatcaacgg cgcgaaaaaa ttcgcccact ccaatggtca
    38581 actgaacgac accctgaccg aagtcgcgga tggcacactg cgcgacctcg ggcatgatgt
    38641 tcgcatcgtt cgcgccgaca gcgactacga tgtcaaagcg gaagtacaaa actttctctg
    38701 ggctgatgtg gtgatctggc agatgccagg ctggtggatg ggcgcgccgt ggacagtgaa
    38761 aaaatacatt gatgatgtat tcaccgaagg tcacgggacg ctgtatgcca gcgatggccg
    38821 tacccgcaaa gatccgtcaa aaaaatacgg ttccggcggc ctggtacagg gcaaaaaata
    38881 tatgctttct ctgacctgga acgcaccaat ggaagccttc accgaaaaag atcaattctt
    38941 ccacggcgtt ggcgtcgacg gtgtgtatct gccgttccat aaagcaaacc aattccttgg
    39001 catggaaccg ctgccgacat ttatcgctaa tgacgtgata aaaatgcctg atgttccccg
    39061 ctatactgaa gaatatcgca agcatcttgt ggaaattttt ggttaactag agctcaggct
    39121 ttagaaggag ttaaccatgc ttaccgtaat cgcagaaatc cgtactcgtc ctggtcaaca
    39181 tcaccgtcag gcggtattgg atcagtttgc taaaatcgtt ccaaccgtac tgaaagaaga
    39241 aggttgccac ggctatgcgc caatggtgga ttgcgctgct ggcgtgagtt tccagtctat
    39301 ggcaccggat tctatcgtga tgattgagca gtgggaaagc atcgcgcacc ttgaagcgca
    39361 tctgcaaacc ccgcacatga aggcgtacag cgaagccgta aaaggcgacg tgctggagat
    39421 gaatattcgt attctgcagc cagggattta atcctgcctt gtttgcccgg ccatcctgac
    39481 cgggcaatgt tctttccttt aaacctcaat ctccgccatg tcgcctttct cttgcaacca
    39541 gttgcggcga tcttccgagc gtttcttcgc cagcagcata tccatcatcg cgtcagtacg
    39601 ctgatcgtct tcatcatcga tagtcaactg caccagacgg cgagtgttcg gatcaagcgt
    39661 ggtttcacgc aattgcatcg ggttcatttc ccccagacct ttaaaacgct ggacgttcgg
    39721 cttgcctttc ttgcgtttta attgctcaag tacgccctct ttctcttctt ccgtcagcgc
    39781 gtaataaacc tctttcccga gatcaatacg gtagagcggt ggcagtgcga cgtaaacgtg
    39841 accgtgtttc accaacgcgc ggaagtgttt tacgaacaaa gcgcagagca gcgtggcaat
    39901 gtgcagacca tcagagtccg catccgcgag gatacagatt ttgccataac gaagctggct
    39961 cagatcgtcg ctgtcaggat cgataccgat cgctaccgaa atatcatgca cttcctgcga
    40021 ggccagcact tcgtcggaag aaacttccca ggtgttaagg atcttacctt tcagcggcat
    40081 gatcgcctga tattcacgat cgcgcgcctg cttggcagat ccgcctgcgg agtcaccttc
    40141 cacgaggaac agttcggtac ggttaaggtc ctgcgcggta caatcagcca gtttgccagg
    40201 cagcgccggg ccgctggtca gctttttacg caccactttt ttagccgcac gcatacgacg
    40261 ctgggcgctg gaaatcgcca tctctgccag caactccgcc gcctgaacgt tctggttcag
    40321 ccacaggata aaggcatctt tcaccacgcc agaaacgaat gccgcgcatt gacgcgaaga
    40381 gagacgctct ttcgtctgcc cggcaaactg cggatcctgc attttcaccg acagaacata
    40441 ggcgcagcga tcccagatat cttccgccga cagcttcacg ccgcgcggca gaatgttgcg
    40501 atattcacag aactcacgca tcgcgtccaa caggccctga cgcaggccat taacatgggt
    40561 accgccctgc atcgttggga tcaggttgac gtagctttcg gtcagcagtt caccgccttc
    40621 cggcagccac agtagcgccc agtccacagc ttcagtatcg ccagcgaaat taccgataaa
    40681 cggtttttct ggcagcgtcg gtaaaccgtt taccgcttcc gccaggtaat cattcagacc
    40741 gtcctgatag caccagcgct gttcggtgtt gttgatctca tctttaaagg taatctcaac
    40801 gccggggcac agtaccgctt tggctttcag cacatgcgtc aggcgtgaaa cagaaaatcg
    40861 cgggctgtca aagaaggttt catccggcca gaagtgcacg ctggtaccgg tattgcgttt
    40921 accgcaagtg ccgacaacct gtaaatcctg caccttttcg ccattttcaa aggcgatgtt
    40981 ataaacctga ccatcgcggc gcacgttaac ttcaacgcgc ttcgacaggg cgttaaccac
    41041 cgaaatcccc acgccatgca ggccgccaga gaactggtaa tttttgttag agaatttacc
    41101 gcctgcatgc agacggcaaa gaatcaattc aaccgccggg acaccctctt ccgggtgaat
    41161 atccaccggc atcccgcgcc catcgtcaat aacttctaac gactggtcag catgtaagat
    41221 tacatccacg cgttttgcgt gacccgccag tgcttcatcg acactgttat caataacttc
    41281 ttgccccaaa tggttagggc gagtggtatc ggtatacatc cccggacggc ggcgaaccgg
    41341 ctcaagcccg gtgagtacct caatggcatc agcgttataa gtttgcgtca tggtttaagt
    41401 tagcaattcg agttgatcgt cagagatggt gcagaccaag aaaatcgacg atcgggttga
    41461 aataatcttc gaagcccgtg aatgcgtggt tgccgccttc tatgacagtc tggcggcagg
    41521 aagcgtagta cgccaccgcc tggcggtaat ccagcacttc atctcccgtc tgttgcagca
    41581 gccagatcaa atccggcgct tccagcgggt caatctgcat gactttgaga tcgtaaatat
    41641 ggcgtgactc tagcacatat tgctgcccgg tgtatgggtt ctcgttctga ccgagatagt
    41701 ccgtcagcag ttcaaacggg cgcaccgccg ggtttaccac cactgcgggc agcataaaac
    41761 attgtgacaa ccaggtggcg taatatcccc ccagtgacga accgacaata cccagcgaat
    41821 cgccgccatg ttccaggaca atggattcca gcagttctgc cgcgtcggaa ggatacggcg
    41881 gcaactgcgg aatgatcatc tcaacgtcag ggtgatgttc cgccagccag ttttttaaca
    41941 agctcgcttt tgcagagcgc ggcgagctgt tgaaaccgtg taaataaaga agcgtagaca
    42001 tcagtagcct tctgaagcgg tatcaggttg gaaacgtgtg tccgccaggc gatgcacctc
    42061 ggtggtcagc gtgccatcag catgtaactc gagagtacgc cagccgggcg cgatagtatc
    42121 cagcgtaaag ttggaacagt gcggcttaaa ctgcacacag gtcgacggcg ttgccagcag
    42181 gcggcgaccg ttccagtcga gatccagctc ctgatgaata tgaccgcaca gcaagtattt
    42241 gacgtgcgga aacttcgcca gcacggtatc cagttcgccc gcgttacgca gactgtgttg
    42301 atcgagccaa ctacaacccg caggtagcgg atgatgatgc agcagcagca acgtatggcg
    42361 ttctggcgca tcggccagtt tacgttccag ccactcaagc tgaaactcgc tcagctcacc
    42421 gtgcggcacg ccaaacacct ggctatccag caacaggatt tgccattgct caccaataaa
    42481 cacccgcttc gccggggaga tacccgcatc ctgtaacgcg ctgtacatcg cgggctggaa
    42541 atcgtggttg cccggcagcc agacgcaggg cgcacgaaaa cttgcgatgc cttcagcgaa
    42601 atgctgatag gccgcagagg attgatcctg cgctaaatca cctgtcgcga caatcaggtc
    42661 gaattcgtgc tgatgtggac gaatcgcctc cagcaccgcc tggtagcttt cccaggtgtt
    42721 tacccctaac agagcttcgt gcttttgtgc aaacaggtga gtgtcggtaa tttgtaaaat
    42781 cctgactctg gcctcaccag ccagaggaag ggttaacagg ctttccaaat ggtgtcctta
    42841 ggtttcacga tgctagtaaa ccggaatcgc catcgctcca tgtgctaaac agtatcgcaa
    42901 ccagtccgct aaaaactgat taatttgatg cttttcgtcg cgttgatgca actttttatt
    42961 aggataatca taccgcgctt tgaagcgaaa aatctgctgg cttgaacaca cttcagccac
    43021 catcgcgtca tgatacagac gcaccgtcat tgacggaagg ctccagtaac tgatcgcggg
    43081 cgcagtctgt tctattgtca ccagggtagt gtatcgggtc gattccacaa tcgtcagccg
    43141 atattgtgcg tttgccacct gatagcttac agtttcgccg ggtgcgtcat tgcgcggtaa
    43201 caaacggcgc aattgtgaaa agttcatctc gcacaggcgc atcatttcag gaaagtcagg
    43261 tgtgtaacgc ttcatttatg cccactcatt ttttaacgct tgatgatgca gctgcagcca
    43321 ttgcaaagcg atgaccgacg ctgcgttgtc gattttcccc tcttctaccc actggtatgc
    43381 ctgttcccgg cttaccacat gaacgcgaat atcttcgttt tcatcagcca gaccatgaat
    43441 accgcttgcg gtcgtggcgt ccacttcgcc caccataatt gacgaacgct cactggtgcc
    43501 ccccgggctt gccaggaaac ttaacaccgg tttggtccgt ttgactatca gtcccgcctc
    43561 ttcaatcgct tcgcgacggg caacatcttc cacactttca ccctcttcta tcatcccggc
    43621 aaccatctcc agtagccagg gggtttcact ggtgtcgtat gcggcaatcc gaatctgctc
    43681 aatcagcaca acttcatcgc gcactgggtc aaagggtagc aagactgcgg cgtgaccgcg
    43741 ctcaaaaatt tcccgccgta cctcatgact catttgcccg ttgaatagac gatgacgaaa
    43801 tctataaaga tctaatgaaa aaaagccgcg ataaagtgtt tctcgtgcaa taatttctac
    43861 atcgtttttg ccaaatgtaa cgggcaggtt gtctggctta agcattgtta atgtcctggc
    43921 actaatagtg aattaaatgt gaatttcagc gacgtttgac tgccgtttga gcagtcatgt
    43981 gttaaattga ggcacattaa cgccctatgg cacgtaacgc caaccttttg cggtagcggc
    44041 ttctgctaga atccgcaata attttacagt ttgatcgcgc taaatactgc ttcaccacaa
    44101 ggaatgcaaa tgaagaaatt gctccccatt cttatcggcc tgagcctttc tgggttcagt
    44161 tcgttgagcc aggccgagaa cctgatgcaa gtttatcagc aagcacgcct tagtaacccg
    44221 gaattgcgta agtctgccgc cgatcgtgat gctgcctttg aaaaaattaa tgaagcgcgc
    44281 agtccattac tgccacagct aggtttaggt gcagattaca cctatagcaa cggctaccgc
    44341 gacgcgaacg gcatcaactc taacgcgacc agtgcgtccc tgcagttaac tcaatccatt
    44401 tttgatatgt cgaaatggcg tgcgttaacg ctgcaggaaa aagcagcagg gattcaggac
    44461 gtcacatatc agaccgatca gcaaaccttg atcctcaaca ccgcgaccgc ttatttcaac
    44521 gtgttgaatg ctattgacgt tctttcctat acacaggcac aaaaagaagc gatctaccgt
    44581 caattagatc aaaccaccca acgttttaac gtgggcctgg tagcgatcac cgacgtgcag
    44641 aacgcccgcg cgcagtacga taccgtgctg gcgaacgaag tgaccgcacg taataacctt
    44701 gataacgcgg tagagcagct gcgccagatc accggtaact actatccgga actggcggcg
    44761 ctgaatgtcg aaaactttaa aaccgacaaa ccacagccgg ttaacgcgct gctgaaagaa
    44821 gccgaaaaac gcaacctgtc gctgttacag gcacgcttga gccaggacct ggcgcgcgag
    44881 caaattcgcc aggcgcagga tggtcactta ccgacgctgg atttaacggc ttctaccggg
    44941 atttctgaca cctcttatag cggttcgaaa acccgtggtg ccgctggtac ccagtatgac
    45001 gacagcaata tgggccagaa caaagtgggc ctgagcttct cgctgccgat ttatcagggc
    45061 ggaatggtta actcgcaggt gaaacaggcc cagtacaact ttgttggtgc cagcgagcaa
    45121 ctggaaagcg cgcatcgtag cgtcgtgcaa accgtacgtt cctccttcaa caacattaat
    45181 gcatctatca gtagcattaa cgcctacaaa caagccgtag tttccgctca aagctcatta
    45241 gacgcgatgg aagcgggcta ctcggtcggt acgcgtacca ttgttgatgt gttggatgca
    45301 accaccacgc tgtacaacgc taagcaagag ctggcaaatg cgcgttataa ctacctgatt
    45361 aaccagttga atattaagtc agctctgggt acgttgaacg agcaggatct gctggcactg
    45421 aacaatgcgc tgagcaaacc ggtttccact aatccggaaa acgttgcacc gcaaacgccg
    45481 gaacagaatg ctattgctga tggttatgcg cctgatagcc cggcaccagt cgttcagcaa
    45541 acatccgcac gcactaccac cagtaacggt cataaccctt tccgtaactg acgacgacga
    45601 cggggcttcg gccccgtctg aacgtaaggc aacgtaaaga tacgggttat ctgccgcatt
    45661 cttccccctt ctcgcttcaa tttcgaccag ccatcctcta ttctgatggg tatttaccac
    45721 tggtcccgga agacaaaaat gaaacggaca aaatccatac gccacgcatc gttccgcaaa
    45781 aactggagcg cacgccatct gacaccagtc gctctcgcgg ttgccactgt ttttatgctg
    45841 gctggctgtg aaaagagtga tgaaacagtg tctctctatc aaaatgctga cgactgttca
    45901 gctgcaaacc caggcaaaag cgccgaatgt accaccgcgt acaacaatgc gctgaaagaa
    45961 gccgaacgta ctgcgccgaa atacgccacc cgtgaagact gtgttgctga atttggtgaa
    46021 ggtcagtgcc agcaggcacc agcccaggct ggcatggcac cagaaaacca ggcgcaggcc
    46081 cagcaatcca gcgggagttt ctggatgccg ctgatggccg gttacatgat ggggcgtctg
    46141 atgggcggcg gcgcgggatt tgcacagcag ccgctgttct cctcgaaaaa cccagccagc
    46201 ccggcttacg gtaaatatac cgacgcgacg ggtaaaaact atggcgcagc ccagccaggc
    46261 cgcaccatga ccgtaccgaa gacagccatg gcaccaaaac cggcgaccac tactaccgtt
    46321 acccgtggcg gttttggtga atctgttgcc aaacaaagca ctatgcagcg tagcgcaacc
    46381 ggcacctctt ctcgttcaat gggtggctga taccgatgga aagagtcagt attaccgagc
    46441 gcccggactg gcgcgaaaaa gcccacgaat acggtttcaa ttttcacacc atgtacggcg
    46501 agccgtactg gtgtgaagat gcttactaca agttgaccct cgcccaggtt gaaaagctgg
    46561 aagaagtcac cgccgaactg caccagatgt gcctgaaagt ggtggaaaaa gtgatcgcca
    46621 gcgatgagct gatgaccaaa ttccgcattc caaaacgcac ctggagtttt gtgcgtcagt
    46681 catggctgac tcaccagcca tcgctttatt cgcgtcttga tctggcatgg gatggcactg
    46741 gcgaacctaa actgctggaa aataacgccg atacaccaac gtcactatac gaggcggcgt
    46801 tctttcagtg gatctggctg gaagatcagc ttaacgccgg taacttgccg gagggcagcg
    46861 accagtttaa cagtctgcaa gaaaagctaa tcgatcgctt tgtcgagttg cgtgaacagt
    46921 atggcttcca gttgctgcat ctcacctgct gtcgcgacac ggtggaagat cgcggaacca
    46981 ttcagtattt gcaggactgc gcgacggaag ctgaaatcgc taccgagttc ctctacattg
    47041 acgatatcgg gttaggtgaa aaaggtcagt tcacggattt acaggatcag gtaatttcca
    47101 acctgttcaa gctgtatcca tgggaattta tgttgcgtga gatgttctcc accaagctgg
    47161 aggatgcagg cgtacgctgg ctggaaccgg cgtggaagag cattatctcc aacaaagcgc
    47221 ttctaccgct actgtgggag atgttcccga atcacccgaa cctgctgccc gcttattttg
    47281 cggaagatga tcatccgcaa atggaaaaat atgtggttaa accgatcttc tcccgtgaag
    47341 gcgcaaacgt gtcgatcatt gagaacggca aaaccattga agcagcggaa ggtccgtatg
    47401 gcgaagaagg gatgattgtt cagcaattcc acccgttacc gaaattcggc gacagctata
    47461 tgctgattgg tagctggctg gtgaacgatc aacctgccgg aattggcatt cgtgaagacc
    47521 gtgcgttgat cacccaggat atgtctcggt tttatccaca tatttttatt gagtaatcaa
    47581 tgatgccgga tggcattcgc catccggtaa ttgttaacct atctgcaccg acagcatact
    47641 caggctgccc atttctatac catcgaccgg aatggtaatt ggctcctgcc catcccacgc
    47701 acctaagaca tacaacaacg gcagatagtg ctccggcgtt gggttcgata acgcgccacc
    47761 ttcatggtca aggtaattca ccagaggatg ctgctctacc gccccttgcc acgtcagatt
    47821 cgctttcaca tactcattaa acgacatcgc ccaaggatat ggtgaactat caccgtgcca
    47881 cttcactgta cgcaggttat gcaccacgtt gccactggcg accaacatta ttccttcatc
    47941 tcgcagcgct gccagtttgc gccccatttc gaaatgccag gcggcaggtt tgctactgtc
    48001 gatactcaac tgcaccatcg ggatatcggc gtcaggatac atcttaatca gcacgcccca
    48061 ggaaccgtgg tcaaagcccc aggcttcttt atccagcgtc accgggaccg acgctaacag
    48121 ctcaaccaga tgctgtgcca gcacaggcga acccggagca ggataatgcg tatcgtacag
    48181 cgcctgcggg aagccaccaa agtcatgaat cgtgggcggc gtctccatcg cggtcactcc
    48241 tgttccacgg gtaaaccagt gagccgaaac caccacaatc gcttgcgggc gtggcaatgt
    48301 catccccaac ttctgccagc tgcgggtata caaattatct tccagcacgt tcatcggact
    48361 accgtgacct aaaaacaatg ctggcatacg tgttgaagac atgatgatat ccttaactaa
    48421 aggtgtcatt ttgatatcct cacaatacgc ttgttcggcg gagtaagaac ccggataaca
    48481 atgatgatga tcatcagtta ttttgacgat ctgcctgaag gtgaagattt ataaggagtt
    48541 gtcgatgtca gtacctctca ttctgaccat actggcgggg gcagccacgt ttattggcgc
    48601 gtttctcggc gttctcgggc aaaaaccctc gaaccgctta ctggcgtttt cgctaggatt
    48661 tgcggcgggg atcatgttgc tcatctcatt aatggaaatg cttcctgccg cactagcagc
    48721 tgaaggaatg tcgcctgtgt tgggttatgg aatgtttatc ttcggtctgc ttggctattt
    48781 tggcctggac cgcatgttgc cacatgcgca tccgcaggat ttaatgcaaa aatcggtgca
    48841 gccgttgcca aaatcgatca agcgcacagc cattctgctc actctcggca tcagtctgca
    48901 taacttcccg gaagggattg ccacctttgt cacggcgagc agcaacctgg agctgggatt
    48961 tggcatcgca ctggccgtcg cgttgcacaa tatccctgaa ggtctggcag tggcaggccc
    49021 ggtttatgcg gcaacggggt ctaaacgcac cgcgattctg tgggcgggga tttctggact
    49081 agcagaaatt cttggtggtg tgctggcgtg gttaatcctc ggtagcatga tttccccggt
    49141 ggtcatggcg gcaatcatgg cggcggttgc aggaattatg gtggcgctct cggttgatga
    49201 attaatgccg ctcgccaaag agattgaccc taataataac cccagctatg gcgtactatg
    49261 tggaatgtca gtgatgggat tcagtttagt gctgctacaa acggcgggaa ttggttaaaa
    49321 acaacatcag aagttgtcca gcacatggac aacttctgcc agcttcacga aagccttatt
    49381 ctaccttttc ggctgtctct tctctcgtac tgtttaagta tttgttccgc gcttcaagca
    49441 gtgcgtctct gtcctggcga aacgcccgac aataacgttt caaatgcagg aaataaccca
    49501 ccacaaccag agcaatcaat acaatccagt accaggcgat aaacatcctt ttacccttaa
    49561 tatttaacaa tactaaacat ttagcgcata aatttcacat atcctttttc ggatatattc
    49621 atatggtcgc ataataacgg aacaattata tgattttgat cataatgaaa aagaatgcct
    49681 gatattaatg aaattttctc atagaaagag aagcggaatt aacatcttgc atagcagggc
    49741 aaaataaaaa ccgaagccag ctacaacttc ggtttcttga ttaaggcagt aattaagcag
    49801 cggttttcag ctggctttac gctcatgtgc ctgacggtat gccaccaggt cttcaatagt
    49861 cacgagcgcc atattgtgtt tattggcaaa ctcaatgcac tctggtgcac gcgccatcgt
    49921 gccatcgtca ttagtcagct cacacagcac accagccggt ttaaagcctg ccagcgtcat
    49981 caaatcaata gtcgcttcag tatgaccgcc acgcgtcagt acaccacctg cctgcgcgcg
    50041 aagtgggaaa acgtggcccg ggcgattcag atcagaaggt tttgcgccat cggcaatcgc
    50101 tgcgcgaacg gtcgtaatac gatcagcggc agaaacaccg gtagtcacac cttcagccgc
    50161 ttcaatggtc acggtaaaac cggtgccata ggcgctggtg ttattttcta ccatcattgg
    50221 cagatcaagt tgtttacggc gatcttcagt gatgcacagg caaacaatac cgctaccgtg
    50281 gcgaatggtc agcgccatct gctcaacggt catggtttct gccgggaaga tcatatcgcc
    50341 ttcgttttca cggtcttcat catcaagcac cattacaccg cgtccttcac gcagcgcagc
    50401 cagtgcattt tcaacacgtt cgaaaggcgt accaaaagag gaaagtagcg tctgattcat
    50461 ggtaaaaaaa cctcactaaa attatggtta ccagaatcag ggcagtctta ggagtggcgg
    50521 catatagcca aaataacgtg agcgggtcca tgcccgacag aatcgttact ctctcccatc
    50581 cggactctaa ccgtcggccc cggaattaca ccggatctgc tgtcctttga gttcgcaccc
    50641 aaagcgctcg cgggctttca actgagttga tttaccgccg gtggggaatt tcgccccgcc
    50701 ctgagaataa gcggattcac tataacgcta atgattagcg gcagcaacgc atagcttcac
    50761 ataattctgg tttatgactt acccttatcg cactacaatg gtactcaaca cctatcagta
    50821 cagggaaact acaatgattg acccgaaaaa aattgagcaa atcgctcgcc aggttcacga
    50881 atcaatgcct aaaggaatca gggagttcgg ggaagatgtg gagaaaaaaa tccgccaaac
    50941 cctacaagct cagctgacgc gcctcgacct tgtaagccgt gaagagttcg acgtccaaac
    51001 gcaagtcctg ttacggactc gtgagaaact ggcgcggctt gaacaaagaa tgagcgaact
    51061 ggaaaatcgc agtactgaga ttaaaaagca gccagatcca gagactctcc ccccaacgct
    51121 gtaaacattt gtctttaatg cactgtaaat tatgaattaa tcttctaatt tacagtgctt
    51181 tctattacct tatcgcttaa ttccattcag aatatcatca caaaaatgaa tgagtgcaat
    51241 tgaaaatata attgttatgt attttttaag ttttacttaa tccatattta gataaactaa
    51301 tttcataaaa cccgaaaagt aaaatataat atttgaatga ttagcaatcc ttaaataaca
    51361 gttactaatc attcattttc tacttatatt tatataaagg aatattatgt ctgcttttaa
    51421 aaagtcactt cttgttgctg gcgtagcaat gattctttcc aataacgtat ttgctgacga
    51481 aggtcacggc attgttaaat ttaaaggtga agttatctct gctccatgtt caattaaacc
    51541 aggtgacgaa gacttaactg taaatctggg tgaagtagca gataccgttc tgaaaagcga
    51601 ccagaaatct ttggctgagc ctttcactat tcacctgcaa gactgcatgt tgagccaggg
    51661 tggaaccact tattcaaaag cgaaggttac tttcaccaca gcgaatacta tgacgggtca
    51721 gactgacctg ctgaaaaaca ccaaagaaac tgaaatcggt ggtgccacag gcgttggcgt
    51781 acgtattctg gatagccaga gcggcgaagt gactttaggt actccggtag taattacatt
    51841 caacaatact aacagctatc aggaactgaa cttcaaagct cgtatggaat ccccgggcaa
    51901 agatgctacc ccgggcaacg tttacgctca ggctgactat aagattgctt acgagtaatc
    51961 acattaggcc gggttcgccc ggccacatca ttaacggatt aatgataagt ggaacagatg
    52021 tataaaaaat taaaattaac cacaataagc gaattgatta aaaatattta ttgttcatta
    52081 tccgttctca tcattggttg tgcgtcagct tatgccgttg aattcaacaa agatttaatc
    52141 gaagccgaag atcgtgaaaa cgttaacctt tcccaatttg aaactgatgg ccaattaccc
    52201 gtaggtaaat actcattaaa cgctcttatt aataataaaa ggacgccaat ccatctggac
    52261 ctgcaatggg tattaattga taaccaaact gcagtttgtc tgactccaga gcaattaaca
    52321 ttattaggct ttactgatga gattattgaa gaggctcagc aaaacctgat cgatggttgt
    52381 taccctatcg aaaaagaaaa acaaattaca acttatctcg ataaagggaa aatgcaatta
    52441 tccatatctg cacctcaggc atggttaaaa tacaaagatg caaactggac gcctcctgaa
    52501 ctttgggatc atggtattgc tggggcattt cttgactaca atttatatgc ctctcattat
    52561 gcaccacatc agggcgataa ttcacaaaat ataagttcct atgggcaggc tggggttaat
    52621 cttggggcct ggcgcctgcg tactgattac cagtacgatc agtcatttaa caatggcaaa
    52681 agccaggcga acaacctgga ttttccgcgt atttatttgt ttcgcccaat cccagcaatt
    52741 aatgcaaaac taactatagg tcaatacgat actgaatcct ctattttcga ctctttccat
    52801 ttttctggcg tttcgttgaa aagcgatgag aatatgttgc cgcctgatct acgtggttac
    52861 gcaccgcaaa tcacgggtgt tgcacaaacg aatgcaaagg tcactgtctc acagaacaac
    52921 cgtattattt atcaagaaaa tgttcctcct ggcccatttg ctattactaa tttattcaat
    52981 acattacagg ggcaactgga cgtcaaggtt gaagaagagg acgggcaagt cacgcaatgg
    53041 caagttgcat ctaatagtat tccttatctg acgcgtaaag ggcagattcg ctacaccact
    53101 gctatgggta aaccgaccag cgttggtggt gattccttac aacaaccctt cttctggact
    53161 ggtgaattct catggggttg gctgaacaat gtatccctgt atggtggttc agttttaaca
    53221 aaccgtgatt atcaatctct ggctgccggc gttggcttta accttaactc attgggttca
    53281 ttatcttttg atgtcacacg atctgatgct cagttgcata atcaggataa agaaacgggt
    53341 tatagctacc gcgctaacta ttcaaaacgt tttgaatcta ccggtagcca gctcactttc
    53401 gctggttacc gtttctctga taaaaacttt gtgacaatga atgaatatat caatgacact
    53461 aaccattaca tgaattatca gaatgaaaaa gagagttata ttgtcacgtt taaccagtat
    53521 cttgaatcat taaggttaaa tacatacgta agtttggctc gtaatactta ctgggacgcc
    53581 agcagtaatg tgaattattc attatcactt agccgcgatt ttgatatcgg gccattaaaa
    53641 aacgtctccg cttctctaac atttagccga ataaactggg aagaagacaa ccaggatcaa
    53701 ctgtacctaa atatttcgat tccctgggga accagtagaa cattgagcta tggtatgcaa
    53761 cgaaatcagg ataacaagat ttcgcatact gcttcgtggt atgactcttc cgatcgaaat
    53821 aattcctgga gcgtttctgc ttcaggcgac aatgatgaat tcaaagatat gaaagcgtca
    53881 ctacgcgcca gttatcagca taataccgag aacggtcgtc tctatctctc cgggacatca
    53941 cagcgagaca gttattattc tctgaatgcc agttggaatg gttcattcac ggcgactcgt
    54001 cacggagccg ctttccatga ctatagtggt agtgctgact cgcgttttat gatcgacgca
    54061 gatggtgcag aagatattcc gttgaacaat aaacgcgcgg taactaatcg gtatggcatc
    54121 ggagttattc catcagtcag cagttacata acaacatcat taagcgttga tacccgaaat
    54181 cttccagaaa atgtggatat cgaaaactcg gttatcacca ccaccttaac cgaaggtgct
    54241 attggctacg ccaaacttga tacccgcaag ggctatcaaa tcatgggggt tattcgcctg
    54301 gcagatggta gtcacccacc actggggatt agcgtaaaag ataaaaccag ccataaagaa
    54361 ttaggactgg ttgctgatgg cggctttgta tacctcaacg gcattcagga tgacagcaaa
    54421 cttactttac gctggggtga taaatcctgt tttattcaac cacccaatag cagcaactta
    54481 accaccggaa cggttatttt accgtgtatt agccaaaatt aattcaggat gaaactaatg
    54541 cgctacttaa acacaaagaa tctcattgca gcaggagtat tactcgcctg catgagcagc
    54601 attgcctggg gtgccatcat cccggaccgt acccgtatca taatgaatga gtcagataaa
    54661 ggggaagcat taaaacttac taatcaaagc aaaaatctac cctatctggc gcaaacatgg
    54721 atagaagata cgaaaggcaa taaatcacgt gactttattg tcacggttcc ccctatggta
    54781 cgtttaaatc ccagtgagca aattcaaata agaatgataa gtcaggaaaa aattgctcaa
    54841 ctgcctaatg acagagaaac gctcttctat tttaacgtgc gagaaatacc gccaaaaaca
    54901 gataaaaaaa atgtgatgca agtcactatg cagcacgcat tgaaactatt ctggcgacca
    54961 aaagctattg aattagaaga tgatggggta atgtcttacg aaaaagttga aatcatccgt
    55021 agaaatgatg gttcgatccg ctttaacaat aagatgcctt atcacgttac gctgggttat
    55081 atcggaacaa acggaatcac aatgttgcca caaacgcaaa gtttaatggt aaccccttat
    55141 agccatgcag atacgcagtt taaaaatgtt ccgtcagctt ttcaggttgg ttatatcaat
    55201 gattttggtg gattaagttt ttatgaaata aactgcccga cggttaataa ctcttgtaat
    55261 gtttccgtag ccaaacgaga taaataatat gcgctactta ttaattgtaa ttatatttat
    55321 tataggtttt agtgcattac ctgtatgggc aatggactgc tatgctgaac atgaaggggg
    55381 aaataccgta gtcattggtt acgtaccaag aatttctatc cccagtaatg gtaaaaaagg
    55441 tgataagatc tggcaaagca gtgagtattt tatgaatgtc ttctgtaata atgcattacc
    55501 tgccccatca cctggagaag aatacccatc agcctggact aatataatga tgtttttacc
    55561 aggcggtcag gacttttata atcagaactc ctatattttt ggcgtaacat acaatggagt
    55621 tgattatgat tcgactgccc ctaatgctat cgcagcacca gaatgcattg atataaaagg
    55681 agcgggtacc tttaataatc actataaaaa ccccgctgtc tgtagtggag gtcctgagcc
    55741 tcaattatca gtaacctttc cagctcgcgt acaactctat attaaattag caaaaaatgc
    55801 caatagagtt aataaaaacc tggtgttacc tgatgaatat atcgctctag agtttaaagg
    55861 tatgagtggc gcaggagcta tagaagtaga caaaaatttg accttcagga ttcgtggatt
    55921 aaataatatt catgtccttg actgctttgt taatgtcgca ctggaaccag ctgatggcgt
    55981 tgttgacttt ggtaaaataa attcccgaac aattaaaaat accagcgtta gtgagacgtt
    56041 tagtgtagtc atgacaaaag atcctggtgc ggcctgtact gagcaattca atattttagg
    56101 gagctttttc actacggata ttttgagtga ttatagccat ctggatatgg gtaatggact
    56161 gctattgaag atatttcata aagatggaac agcaacggaa tttaaccgct tctcacaatt
    56221 tgcttctttt tcatcgtcta gtgcaccttc ggtcaccgca ccattcaggg cagaacttag
    56281 tgcgaacccg gcagagactg ttgttgaggg accgtttagt aaagacgtaa tcctcaaaat
    56341 cacctat
//
LOCUS       DAESHI010000015        55730 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.015, whole genome shotgun sequence.
ACCESSION   DAESHI010000015 DAESHI010000000
VERSION     DAESHI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.015"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..494
                     /gene="gstA"
                     /locus_tag="KW011_000931"
     CDS             <1..494
                     /gene="gstA"
                     /locus_tag="KW011_000931"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HBH7659348.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
     gene            complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="KW011_000932"
     CDS             complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="KW011_000932"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HBH7659349.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="KW011_000933"
     CDS             complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="KW011_000933"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBH7659350.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="KW011_000934"
     CDS             complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="KW011_000934"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HBH7659351.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="KW011_000935"
     CDS             complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="KW011_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HBH7659352.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="KW011_000936"
     CDS             complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="KW011_000936"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBH7659353.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5388..5855
                     /gene="slyB"
                     /locus_tag="KW011_000937"
     CDS             5388..5855
                     /gene="slyB"
                     /locus_tag="KW011_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HBH7659354.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="KW011_000938"
     CDS             complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="KW011_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HBH7659355.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6537..6773
                     /locus_tag="KW011_000939"
     CDS             6537..6773
                     /locus_tag="KW011_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HBH7659356.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6776..7633
                     /locus_tag="KW011_000940"
     CDS             6776..7633
                     /locus_tag="KW011_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBH7659357.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7654..9645
                     /locus_tag="KW011_000941"
     CDS             7654..9645
                     /locus_tag="KW011_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBH7659358.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="KW011_000942"
     CDS             complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="KW011_000942"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HBH7659359.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10248..11144)
                     /locus_tag="KW011_000943"
     CDS             complement(10248..11144)
                     /locus_tag="KW011_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HBH7659360.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11193..11432)
                     /locus_tag="KW011_000944"
     CDS             complement(11193..11432)
                     /locus_tag="KW011_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HBH7659361.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11535..12134
                     /gene="nemR"
                     /locus_tag="KW011_000945"
     CDS             11535..12134
                     /gene="nemR"
                     /locus_tag="KW011_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HBH7659362.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            12171..13268
                     /gene="nemA"
                     /locus_tag="KW011_000946"
     CDS             12171..13268
                     /gene="nemA"
                     /locus_tag="KW011_000946"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HBH7659363.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMEE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13349..13756
                     /gene="gloA"
                     /locus_tag="KW011_000947"
     CDS             13349..13756
                     /gene="gloA"
                     /locus_tag="KW011_000947"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBH7659364.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13859..14506
                     /gene="rnt"
                     /locus_tag="KW011_000948"
     CDS             13859..14506
                     /gene="rnt"
                     /locus_tag="KW011_000948"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HBH7659365.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14599..19168
                     /locus_tag="KW011_000949"
                     /pseudo
     CDS             14599..19168
                     /locus_tag="KW011_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="KW011_000950"
     CDS             complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="KW011_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HBH7659366.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            19900..20715
                     /gene="mepH"
                     /locus_tag="KW011_000951"
     CDS             19900..20715
                     /gene="mepH"
                     /locus_tag="KW011_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HBH7659367.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20843..21424
                     /gene="sodB"
                     /locus_tag="KW011_000952"
     CDS             20843..21424
                     /gene="sodB"
                     /locus_tag="KW011_000952"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HBH7659368.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21570..22739)
                     /locus_tag="KW011_000953"
     CDS             complement(21570..22739)
                     /locus_tag="KW011_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH7659369.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(22905..22994)
                     /gene="ynhF"
                     /locus_tag="KW011_000954"
     CDS             complement(22905..22994)
                     /gene="ynhF"
                     /locus_tag="KW011_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HBH7659370.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            23293..24318
                     /gene="purR"
                     /locus_tag="KW011_000955"
     CDS             23293..24318
                     /gene="purR"
                     /locus_tag="KW011_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HBH7659371.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(24315..25247)
                     /locus_tag="KW011_000956"
     CDS             complement(24315..25247)
                     /locus_tag="KW011_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH7659372.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            25360..26571
                     /locus_tag="KW011_000957"
     CDS             25360..26571
                     /locus_tag="KW011_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HBH7659373.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            26862..28010
                     /gene="cfa"
                     /locus_tag="KW011_000958"
     CDS             26862..28010
                     /gene="cfa"
                     /locus_tag="KW011_000958"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HBH7659374.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="KW011_000959"
     CDS             complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="KW011_000959"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBH7659375.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            28906..30279
                     /gene="mdtK"
                     /locus_tag="KW011_000960"
     CDS             28906..30279
                     /gene="mdtK"
                     /locus_tag="KW011_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HBH7659376.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNKKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(30320..31576)
                     /locus_tag="KW011_000961"
     CDS             complement(30320..31576)
                     /locus_tag="KW011_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HBH7659377.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            31884..31960
                     /locus_tag="KW011_000962"
     tRNA            31884..31960
                     /locus_tag="KW011_000962"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31918..31920,aa:Val,seq:gac)
     gene            31965..32041
                     /locus_tag="KW011_000963"
     tRNA            31965..32041
                     /locus_tag="KW011_000963"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31999..32001,aa:Val,seq:gac)
     gene            32149..32454
                     /locus_tag="KW011_000964"
     CDS             32149..32454
                     /locus_tag="KW011_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBH7659378.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            32580..34184
                     /locus_tag="KW011_000965"
     CDS             32580..34184
                     /locus_tag="KW011_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HBH7659379.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(34196..35008)
                     /locus_tag="KW011_000966"
     CDS             complement(34196..35008)
                     /locus_tag="KW011_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659380.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(35012..35797)
                     /locus_tag="KW011_000967"
     CDS             complement(35012..35797)
                     /locus_tag="KW011_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HBH7659381.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(35794..36462)
                     /locus_tag="KW011_000968"
     CDS             complement(35794..36462)
                     /locus_tag="KW011_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH7659382.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(36526..37164)
                     /locus_tag="KW011_000969"
     CDS             complement(36526..37164)
                     /locus_tag="KW011_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HBH7659383.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(37177..39279)
                     /locus_tag="KW011_000970"
     CDS             complement(37177..39279)
                     /locus_tag="KW011_000970"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HBH7659384.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(39300..39926)
                     /locus_tag="KW011_000971"
     CDS             complement(39300..39926)
                     /locus_tag="KW011_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HBH7659385.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(40382..40591)
                     /gene="fumD"
                     /locus_tag="KW011_000972"
     CDS             complement(40382..40591)
                     /gene="fumD"
                     /locus_tag="KW011_000972"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HBH7659386.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            41148..42560
                     /gene="pykF"
                     /locus_tag="KW011_000973"
     CDS             41148..42560
                     /gene="pykF"
                     /locus_tag="KW011_000973"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HBH7659387.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            42870..43106
                     /gene="lpp"
                     /locus_tag="KW011_000974"
     CDS             42870..43106
                     /gene="lpp"
                     /locus_tag="KW011_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HBH7659388.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(43169..44173)
                     /gene="ldtE"
                     /locus_tag="KW011_000975"
     CDS             complement(43169..44173)
                     /gene="ldtE"
                     /locus_tag="KW011_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HBH7659389.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(44322..44738)
                     /gene="sufE"
                     /locus_tag="KW011_000976"
     CDS             complement(44322..44738)
                     /gene="sufE"
                     /locus_tag="KW011_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HBH7659390.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(44751..45971)
                     /gene="sufS"
                     /locus_tag="KW011_000977"
     CDS             complement(44751..45971)
                     /gene="sufS"
                     /locus_tag="KW011_000977"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HBH7659391.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(45968..47239)
                     /gene="sufD"
                     /locus_tag="KW011_000978"
     CDS             complement(45968..47239)
                     /gene="sufD"
                     /locus_tag="KW011_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBH7659392.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(47214..47960)
                     /gene="sufC"
                     /locus_tag="KW011_000979"
     CDS             complement(47214..47960)
                     /gene="sufC"
                     /locus_tag="KW011_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBH7659393.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(47970..49457)
                     /gene="sufB"
                     /locus_tag="KW011_000980"
     CDS             complement(47970..49457)
                     /gene="sufB"
                     /locus_tag="KW011_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBH7659394.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(49466..49834)
                     /gene="sufA"
                     /locus_tag="KW011_000981"
     CDS             complement(49466..49834)
                     /gene="sufA"
                     /locus_tag="KW011_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HBH7659395.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(50382..50570)
                     /locus_tag="KW011_000982"
     CDS             complement(50382..50570)
                     /locus_tag="KW011_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HBH7659396.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            complement(50670..51080)
                     /gene="menI"
                     /locus_tag="KW011_000983"
     CDS             complement(50670..51080)
                     /gene="menI"
                     /locus_tag="KW011_000983"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HBH7659397.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(51077..54133)
                     /locus_tag="KW011_000984"
     CDS             complement(51077..54133)
                     /locus_tag="KW011_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBH7659398.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            54522..55634
                     /gene="ydiK"
                     /locus_tag="KW011_000985"
     CDS             54522..55634
                     /gene="ydiK"
                     /locus_tag="KW011_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HBH7659399.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
BASE COUNT        13414 a        14653 c        13645 g        14018 t
ORIGIN      
        1 aaaacggtga cgattacttt gccgttaacc ctaaggggca ggtgccagca ttgctgctgg
       61 atgacggtac tttgctgacg gaaggcgtag cgattatgca gtatcttgcc gacagcgtcc
      121 ccgaccgcca gttgctggca ccggtaaaca gtatttcccg ctataaaacc atcgaatggc
      181 tgaattacat cgccaccgag ctgcataaag gtttcacacc tctgtttcgc ccagatacac
      241 cggaagagta caaaccgaca gttcgcgcgc agctggataa gaagctgcaa tatgtgaacg
      301 aggcactgaa ggatgagcac tggatctgcg ggcaaagatt tacaattgct gatgcctatc
      361 tgtttacggt tctgcgctgg gcatacgcgg tgaaactgaa tctggaaggg ttagagcaca
      421 ttgcggcatt tatgcaacgt atggctgaac gtccggaagt acaagacgcg ctgtcagcgg
      481 aaggcttaaa gtaatagcca tcggcccaca ggtcgttgtg ggccgaaatg tgatatttca
      541 gagctttgtt gcgctgaagt aatgttctgg tttggcaata cgatcctgag cagccaccac
      601 ttgcagctca tattcctgca ttgctttggt ggtcaccatg atttcgtaga ctgcagcggt
      661 cacatgttcc aggacctcct gcagcgttgc cccctgaagc agtttcacca gcagtaaacc
      721 gctcgtcaca tcaccaacac ctaccggctg gcgcatacca aaatccacca gtggacggct
      781 gatatgccag gcttcatcgg cggtgaccag cagcatttca aaacggtcac ggctgtagcc
      841 agctcgcgcc aggtgtttaa ccaacacaat ttgtggccct tgcgcaatga gttcgcgcgc
      901 tgccagaacc gcttcttcga cgttatttac cgcatgctca cagagtattt ccagctcaac
      961 cagattcggc gcaatgatat cgctggcagg caaaccgtgc cgcacatgaa actctgcgac
     1021 acccggtgca acgatacagc ctttttccgg atgccccatt accggatcgc aaaaatattt
     1081 cgcctgcgga ttcgctgctt tcacctgacg gacgataccg aggatatgtt caccctgctc
     1141 cgccgatccc agatagccac ttaacacggc atcacaggtg tgtaatttat caatagcggc
     1201 aatgccttgc acaatttcgg ttaaatggct gggcggcatc acgcagccag tccatttgcc
     1261 gtattgggtg tgattagaaa attgaacggt gttcagcggc cagacgttcg cgcccaggcg
     1321 gcgcatcgga aactctgccg cactgttacc cgcatgacca taaacaacgt gagactggat
     1381 agcgagaata tttttcatca tgttccctgt ataaaaacca gggggagtga tttctcactc
     1441 cccctttcca cttaatgcat tatttccagc aaatcagaca gtaattcttt ttgccgcgac
     1501 gcagtaaggt aaaacgacca aacagacgat cttcttcttt aaagaagtat tcaggatcgg
     1561 actgtttttc accgttaatg gtgatggcat tggaggcgat agttttacgc gcctgaccac
     1621 gggaaggttg cagttcagaa tcgaccagcg cctgcatcag gtctgcgccc ttttccatct
     1681 caaccatcgg tacgccgtcc tgcgccagct gttcgaagtc cgcttcactc agcgcactca
     1741 aggaaccgct gaacaggcat tcggtaatac gttttgccgc ctgcaaacct tcttcaccgt
     1801 gaaccagacg agtcacctgc tccgccagta catactgggc gcgcggtgct ttaccgctgt
     1861 ttttatcttc ttcttccagg gcgttgatct cttcaatgct cataaaggtg aagaacttca
     1921 ggaagcggta aacgtcggca tccgcagtgt tgatccagaa ctggtagaat ttgtacgggc
     1981 tggttttctt cggatccaac cagactgcgc cgccttcagt tttaccaaat ttggtgccgt
     2041 ctgctttggt gatcagcgga acggtcaggc caaacacctg attctgatgc agacgacggg
     2101 tcaggtcgat accagaagtg atgttacccc actggtcaga accaccaatt tgcagcacca
     2161 caccgtactg tttgttcaga caggcgaagt cataaccctg caacaggttg taggaaaact
     2221 cagtgaacga aatcccctga tcttcacggt tgagacgctg cttaaccgct tctttgttga
     2281 tcatctggtt aacggagaag tgtttgccaa tatcgcgcag gaaggtcagc acattcatat
     2341 tgccgaacca gtcatagttg ttcgccgcga tagcagagtt ttctccacag tcgaaatcga
     2401 ggaacggggc aacctgcttg cggattttgt ccacccactc ctgaacagtt tcttcggtgt
     2461 tcagcttacg ctcggcagct ttgaagctcg ggtcgccaat cagacccgtc gcgccgccta
     2521 ccagcgcaac cggcttgtgg cccgcctgct ggaagcgttt caggcataac aatggaacaa
     2581 gatgccccaa atgcaagctg tcagcggtag gatcgaagcc gcaatagagc gcgatcgggc
     2641 cttgcgccag tcgctctgct aacgcttcct cgtccgtcac ctgggctacc agcccccgct
     2701 cttgcaattg tttaatcaag ttactgcttg ccatcaaaat ctccatgtat ataacgactg
     2761 cacctttgcc ggtacacgac ttttcgccag atgcgaaaga gacatagaat aaagtgccag
     2821 aatcaggagt accagcgatt aaagcaagat ttttgcatct tttcagggtg caagacgatc
     2881 aatcttccac gcatcatttt cacgctggta caaaaagcgg tcatgcaggc gatgctcacc
     2941 gccctgccag aactcaatct gttcaaggct gacgcgaaaa ccgccccaaa agctcggcaa
     3001 tggcacttcg ccctgttgaa acttctgctt cagctccagg aatttacttt caaggatacc
     3061 gcgggcagaa atgcgactgg actgcttcga aacccatgca ccaatctggc tatcacgcgg
     3121 gcggctatga aaatatttca tcacttcgag agtcgaaagt cgttccgctt taccgatcac
     3181 catcacctgg cgctcaaggg tatgccacgg gaacagcagg ctaacgcgcg gattattttc
     3241 aatttgatgt gctttacggc tgccgaggtt ggtgtaaaac accatgcctt tttcgtcgta
     3301 atgtttgagt aaaacgatgc gttgataagg ctgagcatgt tcatccacgg tagcgaccac
     3361 catcgcggta gggtctgcca gtttggcttc acacgcctga gagagccaac gttcaaaaag
     3421 ggttaatgga tcggcgggaa gatcgcgacg gcgtaacccg cctttggtgt attcacggcg
     3481 cagatgcgcg atttgctgca attcgtcgtt atcagacatg gttttcttta cggattgtca
     3541 gtgggtgacg ctattgtgcg ccgcccctgg aaaaatctca acgctgtgga ttttgtaact
     3601 gacagttatt caatacgatg cggtcgcgtt tatagacagt cgcttcatcg cctttcgacc
     3661 agaaaacata gattccgtca gtgtaacgtg caccagacgc tgaaatgccc tgtttgagat
     3721 gcagtagttg attatcgtaa acaaaactga cttcctggcg cggattattc agtttgaccg
     3781 tcaacggttt ttcatcacac tggtattcca gcgtagcggt ttgcatgtgc tcaaccagtt
     3841 gattgaacgc gctacagccg gtgagaagca ccggcaaaca gatcagtaac agttttttca
     3901 tagacatttc ccgaagactt tcctggtctg gagggcaata cgccctccct aacgtttcaa
     3961 gtgtaacggc agacgcggta agaaaaattc agttaactct gatatcacgg gttagcaggg
     4021 aaaatagccc ccagtaccgt ctcctggctt gcgccagtga cggaaggcag atttcctggt
     4081 aatcccgcca gcgtccgcca ggcaagccag gcgaatgcca atgcttccat gtcatcgcca
     4141 ctaatgccaa cggcatcggt ggtggtgact tctgtgcctg gcagtaatgc cgccagacgc
     4201 gccatgagta gcgggttacg actacctcca ccacatacca tcaatcgttc gcagccaccg
     4261 ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt ctgccagtgt ggcctgaaca
     4321 tcacggggat caacacccgg aaaatggcgt aaatggcgct ccagccagcc gtagttaaag
     4381 tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat atgggtcgct gagcatattt
     4441 tgcagcagtg ggagaataac tttacccgcc cgtgcccact cggcatcttt atcgtaaggt
     4501 ttaccggcct gacgccagat ccaggcatcc atcagcatgt taccaggacc ggtatcgtag
     4561 ccgccaaccg gctgcccagg aatgagcagt gacagattgg cgatgccgcc aatattgagc
     4621 accattcgtc gctcggttgg gtgagccagc aaggcatgat ggaacgcagg taccagcggt
     4681 gcgccttgtc ctcccaaggc aatatcgcga cggcgaaaat caccgacaac cgtaattccg
     4741 gtgcgtgcca caatttgatt gttatcgcca atctgtaaag tgtgtggtgc cacgcccgtc
     4801 ggttcatgcc agacggtttg accgtgacag ccgatcgcaa ctatatctct cgcctgcagg
     4861 ttttgttcct taagcaaggc attgaccgca tcagcaaaaa gtcgcccgag ttgagtatca
     4921 agctgtccaa actgcgaaag tgtaagctgc tggccctggc aaatatccag tacagcctgt
     4981 ttcagagaca ccgggatcgg ccagctcaag cttgccagct gtgcgacccg gtgttcatct
     5041 attgtcgcca acacaacatc aacaccatca aggctggtgc ctgacataac gccaataaag
     5101 cggcccgatt tcatagttca tcctttttca atctgacgtt tgcgcaccac tcaaacataa
     5161 acttttcgtg gataccatgc ggaatgaccg atttttaccg ttggtagtaa aacattatct
     5221 tcaaatcaat aatcatcatg aatgttttgt ttataattgg ttgatcctac tttcattatg
     5281 atttgctcat atttggtaga acatgtaacc atggattcac atatgccata tactttgacc
     5341 atgagggatg cttgcgtggc gtttcatggt gaacaggaga tttttcaatg attaaacgcg
     5401 tattggttgt ttcaatggta ggtctgtctc ttgtcggttg tgttaataac gacaccctgt
     5461 caggggatgt ttataccgct tctgaagcga aacaagtaca gaatgtcagc tatggcacca
     5521 tcgttaacgt acgtccggta cagattcagg gcggtgatga ttccaacgtt atcggtgcaa
     5581 ttggcggtgc tgttcttggt ggtttcctgg ggaatactgt tggtggcgga accgggcgtt
     5641 ctctggctac tgcagcaggc gctgttgcag gtggcgtagc tggtcagggc gtacagagtg
     5701 caatgaacaa aacgcagggt gtcgagctgg aaattcgtaa agacgatggt aataccatca
     5761 tggtggtaca gaaacaaggc aacactcgtt tctctccggg ccaacgtgtc gtactggcca
     5821 gcaatggcag tcaggtgacc gtttctccgc gctaaataag tttgcgtgtg gtcaggttac
     5881 tgaccacacg cccccttcat ttcacccttt ggcctgtaac tcaatgatat tatgctcaag
     5941 ttttgcgatg agcgtaatca gttgctccag ttcctctgcg gagatgccat gtaatatttc
     6001 cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc agtggctctg ccttttccgt
     6061 cagtttaata cgtttagccc gacgatcgct ggcacaagtt tgacgcgaaa ttaacccttt
     6121 ttcttccagt tggtccagag tacggaccag tgatggctgc tcgatgccaa tcgcttttgc
     6181 cagttgaatt tgcgactggt ctggaggtaa ctgatggata ttgtgtaacg taacccaatg
     6241 ggtttgtgtt aactccagcg gtttcaggcg atggtctatc agagcacgcc atatgcgcac
     6301 caaccgtgcc agatcagaac ctagtggcga ttccaatttc atctccttat aattagcttg
     6361 ctaagatatt atgcggcttt tagaatagtg tgcagcaatt gtattgctaa aacaaatgta
     6421 ttgctgcatt tggttaccgt cagacatatt tttcagaaat tgcgcgtaaa tttttcgcac
     6481 ttaaagaata tttattaatc taacgcaata tattcggtcg taaaggaatc tactttgtga
     6541 agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt gcgtccgtct
     6601 attttcctcc gtttttcaaa gcattcgcgt ttggattcgt catctggctt gtcgtacacc
     6661 gcctgcttcg tggctggatc tacgccggtg acatctggca tcccttgtta atggatttat
     6721 cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg taactatgtc
     6781 aattaaaaca attaagtatt tctcaacaat cattgtagcg gtagttgcgg ttcttgccgg
     6841 atggtggctg tggaattatt acatgcaatc tccgtggaca cgcgacggga aaatacgcgc
     6901 agaacaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga atataaaaga
     6961 caaccagctg gttaacgcag gggatcttct gctcacgatc gataaaacgc cctttcagat
     7021 cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa tctgacctcg caaaagccaa
     7081 taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttctgccg aagaactcga
     7141 taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac aggccacgct
     7201 gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga gtggacgggt
     7261 gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc tttttgccct
     7321 tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt tgcgccatat
     7381 ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt tacagggtca
     7441 cgtttccagt atcggtcggg cgatttatga tcagagcgtt gaaagtgact caagccttat
     7501 tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caacgcgttc ccgtccgctt
     7561 tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct gtagcattgc
     7621 cgtaggtcaa taatgaacgc atcgtaatgg tccttgcgca atttgccctg gttcagggcc
     7681 acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct ggcgctgacg
     7741 gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc tgcagtggtt
     7801 agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatcgc tggcagttta
     7861 ctgggagcca ttgcggcact gcttcttgcc ggacatacgc tcaatgagcc gtggtttttt
     7921 ctattgagca tgtcggcgtg gcttggcttt tgtacctggg cctgtgcgca cttcacgaat
     7981 aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc ctttccgatg
     8041 gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt ttgcgaggtg
     8101 atagtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag ttccgatgct
     8161 actgcccttt taactgcatt gaaaaacatg cacgcccgat tactggaaca tgccagttta
     8221 ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac atgagggggt gattgggcag
     8281 atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg ttttcgccag
     8341 caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag tgtcatctcc
     8401 agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg agaaattctc
     8461 gaacaattgc tgactgcgct cgccagttcg caaacagatg cttacaccgt cgcacgtatt
     8521 atcgccccgc tacgcccgac caacgtcgcc gactaccggc acgtcgcctt ctggcagcga
     8581 ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg tctgcaaagc
     8641 ggtgtagatg atcatgccag actcgcacgg acatccgctc tggctcgtca taccgacaac
     8701 gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat tggcgcatgg
     8761 agtattgcat cgcaatggga tgccggtgcc aatgcattaa cgctggcagc aattagctgc
     8821 gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat gcgcacgctg
     8881 gtgttacttt cgctattcag ctttgtggtc aaatttggcc tgatggtcca gattagcgat
     8941 ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct tcttaaattg
     9001 cagatgccca aatttgccgc actgtggggg caactgattg tttttatggg ttcgtttatc
     9061 gctgtcacta atcccccggt gtatgatttt gctgattttc ttaacgagaa tctggcaaaa
     9121 atcgttggcg ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg atcggatgct
     9181 cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca gctaagccgc
     9241 catccaacac tgagtggaag cgaatttgaa tcgctcactt atcatcacgt cagtcagttg
     9301 agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt agtgctgctg
     9361 aactgttctc atgttgtctg gcaattgcgc gactgggaat cgcgttccga tccgttatcg
     9421 cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg tggcgttcag
     9481 caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag ccttgcccgt
     9541 catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta ctgctcgctt
     9601 tcgcaacttg agcaagcacc accgcaaggt acgctggcct cgtaattact taattacacc
     9661 acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca tattatcgcc
     9721 gccaacgtgg accatcagcg ccttgtcttt gatttcatcc agtgatttca gacgaggcgc
     9781 gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca gatcgcctaa
     9841 atgcccggta ccttctggcc cttcatgttt accggtattt tgtggatcaa gatgcccgcc
     9901 tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc ttcctttggc
     9961 atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggtg aaaactccag
    10021 acctttatcg gtttcagtaa tggtgacgct accaattgac tgcccaaccc cttgcgacgt
    10081 gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggtcgcaa caaccagcgc
    10141 cagaatggcc agactaaaac gtttcatagg acctccgttc attgtgcatt cctgaataag
    10201 tgtaaaccag tgacacattt ttgaacggcg gctattccta aaagtgctta cggtacgtcg
    10261 taccccagtg ccgctttacg gatacgaaac cattgttgac gggtcatttt cagtgtttct
    10321 gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc cgataattgg cagcggctgc
    10381 gacggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc gtttaactcc
    10441 tccgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt aaacagacga
    10501 ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgtag ttggtcgagc
    10561 gtgccatcca gcagtaacgg ctgatgcacc ggggatattt ccacctgatt agtggcaagg
    10621 gtaaacggca gacgtgattt caacagggca aattgcgcag gcgtaaagtt cgatacgcca
    10681 aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac ttcatcggca
    10741 tccattaacg ggtctggtcg gtggattaac agcagatcca gatgatcggt cgcgagatta
    10801 attagcgact gttcggcgct cttaatgatg tgatcgcggt cagtgatgta atgaccaatg
    10861 acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc catccgttca
    10921 cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg atagccacca
    10981 taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc aataaaactg
    11041 accagctggc gggcggacat attccagtcc atcaatcgcc agtagcccat cacaaaacgg
    11101 gaaaactccg ggccttgcgg cgcaatagta atacgctgaa ccataatcgc ttcctcttat
    11161 cagatatgag aggagtatac gcaagattag gttcaaaaga gtgatggttg ctccggttcg
    11221 tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg acaaaggcgc
    11281 agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc atcccgacta
    11341 cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg gcacgggctc
    11401 tggacgggaa agaactctaa ttgctccgcc acttcgccct cctcaaataa gattattacc
    11461 attattgaag ctgttaatgt ccaaagtagc aactttgctt gcactagacc gactggtcta
    11521 ctacactcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat ctcctggcga
    11581 cgggcgagca actttgcctg caacgtgggt tcaccgggat ggggctaagc gaattactaa
    11641 aaatcgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa gaagcgtttg
    11701 gcgttgctat gcttgagcgc cattacgcca catatcacca gcgactgact gagttgctgc
    11761 aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa acactgaacc
    11821 agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct gccgaagtgt
    11881 gcgatctgtc agaagatatg cgtagcgcga tggataaagg cgctcgcggc gtgattgccc
    11941 tgctctctca ggcgctggaa aatggccatg agaaccactg tttaaccttt tgtggcgaac
    12001 cgctgcaaca ggcacaagtg ctttacgcac tatggctggg tgcgaatctg caggccaaaa
    12061 tttcgcgcaa ttccgagcca ctggaaaacg cgctggcaca tgtaaaaacc attattgcga
    12121 cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt atgtcatctg
    12181 aaaaactgta ttccccactg aaagtgggcg caataactgc ggcaaaccgt atttttatgg
    12241 caccgctgac gcgtctgcgc agtattgaac cgggtgacat ccctaccccg ttgatggagg
    12301 aatactatcg ccaacgtgcc agtgccggtt tgattattag cgaagccacg caaatttctg
    12361 cccaggcaaa agggtatgct ggtgcacctg gcatccatag tccggaacaa attgccgcat
    12421 ggaaaaaaat taccgctggc gttcatgctg aaaatggtca tatggccgta cagctgtggc
    12481 acactggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt
    12541 cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag gcgatccgtg
    12601 ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccaggtatc gtcaatgatt
    12661 tccgtcaggc cattgctaac gcgcgtgaag ccggttttga tctggtagag ctccactctg
    12721 ctcacggtta tttgctgcat cagttccttt ccccttcttc aaaccatcgt accgatcagt
    12781 acggcggcag cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat gccgggattg
    12841 aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact ttccagaaca
    12901 cggataacgg cccgaatgaa gaagccgatg cactgtatct gattgaacaa ctgggtaaac
    12961 gcggcattgc ttatctgcat atgtcagaac cagattgggc gggaggtgaa ccgtatactg
    13021 atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc gcaggtgcat
    13081 acacggtaga aaaagccgaa acgctgattg gcaaagggtt aattgatgcg gtggcatttg
    13141 gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagcgcaga gctgagctta
    13201 acccacagcg tgccgaaagt ttctacggtg gcggcgcgga aggctatacc gattacccga
    13261 cgttgtaatc caacattgcg agcggcgtaa agccgccgct atactaaaac aacattttga
    13321 atctgttagc catttttagg ataaaaagat gcgtcttctt cataccatgc tgcgcgttgg
    13381 cgatctgcaa cgctccatcg atttttatac caaagtgctg ggcatgaaac tgctgcgtac
    13441 cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc cggaaaccga
    13501 agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac tcggcactgc
    13561 ttatggtcac atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa aaatccgtca
    13621 aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta cggttatcgc
    13681 gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagagaaag acgccggtcg
    13741 cggtctgggc aactaatctc ctgccgggcg tgaactcatc gcgcccgcat ctttactgca
    13801 tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag agaatcagat
    13861 gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt atcctgttgt
    13921 gatcgatgtt gaaacagccg gatttaacgc caaaaccgat gcgctgcttg agattgccgc
    13981 catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat tacatttcca
    14041 cgtcgaacca tttgtcggcg caaatttgca gccagaggcc ctcgccttca acggcattga
    14101 cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg aaatttttaa
    14161 agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg tggcgcacaa
    14221 tgccaatttt gatcacagct ttatgatggc cgccgcagaa cgcgcctcac tgaaacgtaa
    14281 cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg cactcggaca
    14341 aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca cccaggcgca
    14401 ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg tcaaccgctg
    14461 gaaacgtctg ggaggctggc cgctacctgc cgccgaagag gtgtaatcga gtcgatgcct
    14521 gatgacatgc aatgattcag gcatctatag tgaggctatt ccacgcatca agcatgatat
    14581 tcacggggaa tagcgttaat ggcagataat ccagaccctt catcgctcct gccggacgtg
    14641 ttttcactgg cgacccgcga ctggtttctt cgcgccttta aacagccgac cgctgtccag
    14701 tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc accgactggc
    14761 tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg cgaaggcggc
    14821 gaagataccc gcgaggcgca taagcgtaaa acctcacgca tcctctatat ttcaccgata
    14881 aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaggg tattgccgat
    14941 gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg tagggatccg tactggcgat
    15001 acgcctgcac aggaacgcag caaactcacc cgtaatccgc cggatattct gatcaccaca
    15061 cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg aaacgctacg cggcgtcgaa
    15121 acggtaatta ttgatgaagt ccacgcggta gcgggcagta aacgtggtgc gcatctggcg
    15181 ttaagtctgg agcggctcga tgcgctgctc cacacctcag cacagcgaat tggcctttct
    15241 gccactgtgc gctcagccag cgatgtggca gcatttcttg gtggcaatcg cccggttacg
    15301 gtagtcaacc cgcccgcaat gcgccatccg cagatacgaa ttgtcgtacc ggtcgccaat
    15361 atggatgatg tctcatcggt cgccagcagc accggcgaag acagccatgc cggccgggaa
    15421 ggctccatct ggccatatat tgaaacgggt atccttgatg aagtgttgcg ccatcgctcg
    15481 accattgtct ttactaattc acgtgggctg gcggaaaaac tgacggcacg attaaatgag
    15541 ctttacgccg cacgcttaca gcgttccccg tctatcgccg ttgatgcggc ccatttcgag
    15601 tcgacctccg gcgcaacctc taaccgtgta caaagtagcg acgtttttat tgcccgctca
    15661 caccacggct ccgtctctaa agaacaacga gcaatcaccg agcaggcgct gaaatcgggt
    15721 gagttacgct gcgtggtcgc aacctccagt ctcgaactgg ggattgatat gggcgcagtg
    15781 gatctgatga ttcaggtggc aacgccgctt tctgttgcca gtgggttaca acgcattggt
    15841 cgcgccggac atcaggttgg cggtgtatct aaagggctgt ttttccctcg tacccggcgt
    15901 gacttagtcg attccgcagt cattgtagag tgtatgttcg caggcaggct ggaaaacctg
    15961 acaccgccgc ataatccact cgacgtcctt gcgcagcaaa ccgttgccgc cgcggcgatg
    16021 gatgcattac aggtagacga atggtactcc cgcgtacgcc gtgccgcacc gtggaaagat
    16081 ctgccaagac gtgtttttga cgctacgctg gatatgcttt ccgggcgcta tccctctggc
    16141 gatttttctg cttttcgccc caaactggtc tggaaccggg agaccgggat attgaccgcc
    16201 cgacctggcg ctcaattgtt ggcggttacc agcggtggca ccattccgga tcgtggcatg
    16261 tatagcgtgt tattacccga aggtgaagaa caggccggtt cgcggcgggt gggtgaactg
    16321 gatgaggaga tggtatatga gtcgcgggtg aacgacatta tcactctcgg cgctacctca
    16381 tggcggatcc agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct
    16441 gcccggctcc ccttctggcg tggtgaaggt aacggacgtc cggctgaatt aggcgagatg
    16501 atcggcgatt ttcttcattt gctggcggat ggcgcgttct tttccgggac tattcccccg
    16561 tggctggcag aagaaaatac gatcgccaat attcaggggt tgattgacga gcaacgcaac
    16621 gcgacgggca tcgttccggg gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt
    16681 ggcgactggc gtattatttt gcactctccc tatggaagac gggtgcatga accctgggcg
    16741 ctggcgattg ccgggcgaat acatgcccta tggggcgctg acgcgtctgt ggtcgccagt
    16801 gatgacggca ttgttgcgcg cattcctgac actgatggta aattgcccga cgccgcgatt
    16861 tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg cagctcggca
    16921 cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat tattaatgcc ggggcgcact
    16981 ccgggccatc gcaccccgct ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc
    17041 gctcagggat atccggattt tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat
    17101 gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa
    17161 atatccgatg taacgaccac tacgccctcg cctttcgcca caagtttatt gttcggctat
    17221 gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag agcgccgggc atccgtactg
    17281 tcgctggaca gcgagttact gcgcaatcta ctcggacagg tcgatccggg ggaattactc
    17341 gacccgcagg tcattcgcca ggtggaagaa gagttgcaac gactggctcc tggcagaaga
    17401 gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa
    17461 gacctggcgc aacggcatac aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt
    17521 cttgcagtaa aacgaatctt cccagcgatg attagcggac aggagcgtct tgcctgtatg
    17581 gatgatgccg ccaggctgcg tgatgccctc ggcgtacgac taccagagtc attgccagag
    17641 atttatttac atagagtcag ttacccgctt cgcgacctct ttctgcgcta tctccgggct
    17701 catgctctgg tcacggctga acaactggct catgagttta gtctcggtat tgccattgtc
    17761 gaagagcagc ttcagcaact gcgtgaacag ggtctggtga tgaatctgca acaagacatc
    17821 tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa
    17881 gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc tgctggaacg tcagggcgta
    17941 ttacccgcca ccgatggtag cccggcgctc tttgcctcaa catcgccagg cgtttatgag
    18001 ggcgtagatg gcgtgatgcg ggtgatcgaa cagcttgccg gagtcggttt acccgcctca
    18061 ctctgggaaa gccagatcct gcctgcccgc gtacgcgact attcgtcaga aatgctcgat
    18121 gaattactgg caaccggtgc ggttatctgg tcggggcaaa aaaactgggt gaagatgacg
    18181 gcctggtggc actgcatcta caggaatatg ctgcagaatc gttcactccc gacgaagcgg
    18241 atcaggcgaa tcgttcggcg ctgcaacaag cgatagtcgc tgttctggct gacggaggag
    18301 cctggtttgc acaacaaatc agccaacgga tacgcgacaa aatcggcgaa tcggttgatc
    18361 tctctgccct gcaagaggcg ttatgggcgc tggtctggca aggcgtcatc accagcgaca
    18421 tttgggcacc gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc
    18481 gcagtcaccg ggctcgtcgt ggacgtcctg tctatgcgca acccgtctcg ccgctggtat
    18541 cttacaacac accaaatctg gctggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    18601 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    18661 gcaaacgctt tgtcgaagta tggaagactc cgggcgaatt atgcgaggtc gttttgtaga
    18721 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    18781 gacacaagcc acgcaaacgc gccactatac accagtggcg ctctctgcca acgatccggc
    18841 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg ttccaacgcg
    18901 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    18961 tggcaaaaaa atgctggtct ggcaggaaaa agaggaatta ctcgccccag aggttttcca
    19021 cgcgctgact accgcactgc gtcgcgaacc acggctgcgc tttacgctaa cagaagtgaa
    19081 tgatctaccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    19141 ttcgccacaa gggctggatt ggggatagag aaaggactga cggatgcccg ttcgcatccg
    19201 tcagtattgc aggacggatt attccgcgtc cggctcttca gacttgtatt tagcggcagt
    19261 ttctttgatc agctgctgca gttcgccacg ctgatacatt tcgatcacga tatcacaacc
    19321 gccgaccagc ttgccgtcaa cccacagttg cgggaaggtc ggccagttag catatttcgg
    19381 cagttccgca cgaatgtccg gattctgcag aatatcaaca taggcaaaac gttcgccaca
    19441 tgcggcaagc gcctggactg cctgggcaga gaaaccgcag ctcggcagtt tcggtgaacc
    19501 tttcatgtac agcaggatcg ggttttcagc aatctggcgt tggatttttt cgatagtggt
    19561 gctcattgtc ttgcttcctc aaacttcttt acggcagtaa tctgacattg tagcgggtca
    19621 gtgcggcatc ggaaaataac attttcatca cgcttttgct attttatccc tttgctctat
    19681 ttgttgcatt tcaaatattc gtttcttctc tgatgccggc aaactggcgt atttataacc
    19741 atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat tttttaacaa
    19801 taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt gtaaatcaga
    19861 cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat aaaccgtatt
    19921 tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat ggcccatgct
    19981 tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc agataaaaag
    20041 aaaagcacgg caaccaccaa aaagacccag aaaacagcga aaaaagccgc cagtaaaagt
    20101 acgaccaaaa gcaaaaccgc ttcttccgtt aaaaaatctt ccattaccgc ttctaaaaac
    20161 gccaaaactc gcagcaaaca cgccgtcaat aaaacggcct cagccagctt taccgaaaag
    20221 tgtaccaaac gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc cgcgtcagga
    20281 actcttgccg acgcgcacaa agcgaaggtg caaaaagcta cgaaagtggc aatgaataaa
    20341 ctgatgcagc aaattggtaa gccatatcgt tggggtggca gctcaccgcg taccggtttt
    20401 gattgcagcg gcctggttta ttacgcttat aaagatttgg tgaaaattcg tattccgcgt
    20461 acggcgaatg aaatgtatca cctgcgtgat gcagcgccaa tcgaacgtag tgaactgaaa
    20521 aacggcgacc tggtcttttt ccgtactcag ggacgcggca cagccgatca tgtcggcgtg
    20581 tatgtcggca acggcaaatt tattcagtca ccgcgcacag gtcaggaaat tcaaatcact
    20641 tctctcagtg aagactactg gcagcgccac tatgttggcg ctcgtcgggt aatgacccca
    20701 aaaacacttc gctaaaactt taccctgttg ttacggcaac agggtaagtt catcttttgt
    20761 ctcacctttt aatttgctac cctatccata cgcacaataa ggctattgta cgtatgcaaa
    20821 ttaataataa aggagagtag caatgtcatt cgaattacct gcactaccat atgctaaaga
    20881 tgctctggca ccgcacattt ctgcggaaac tatcgagtat cactacggca agcatcatca
    20941 gacttatgtc actaacctga acaacctgat taaaggtacc gcgtttgaag gtaaatcact
    21001 ggaagagatt attcgcagct ctgaaggtgg cgtattcaac aacgcagctc aggtctggaa
    21061 ccatactttc tactggaact gcctggcacc gaacgccggt ggcgaaccga ctggaaaagt
    21121 cgctgaagct atcgccgcat cttttggcag ctttgccgat ttcaaagcgc agtttactga
    21181 tgcagcgatc aaaaactttg gttctggctg gacctggctg gtgaaaaaca gcgatggcaa
    21241 actggctatc gtttcaacct ctaacgcggg tactccgctg accaccgatg cgactccgct
    21301 gctgaccgtt gatgtctggg aacacgctta ttacatcgac tatcgcaatg cacgtcctgg
    21361 ctatctggag cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa atctcgctgc
    21421 ataataactg atggcaaatg cagcattgcc tgatgcgctt cgcttaccat gccaacgcgg
    21481 atgaggcgtt aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa ctgtaacgca
    21541 gaaggttatc cttctgcgtt tttgtttaat tagctgttgg caacgcaaac tgtttcaggt
    21601 tgttttctgg ctgacataaa caccagcaat aatgccagtc ccgcgacaat cgcccccatc
    21661 accggcacaa agctgtatcc cagcccagcg gaaattaccg caccaccagc cgctgctccc
    21721 agcgcatttc caagattaaa ggcaccaata ttgactgatg aagacagacc tggcgcttca
    21781 ctggcgacac gcatcacgcg catctgtaac ggcggtacga ccgcaaaggt tgctgcgccc
    21841 cacaccacca tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg ggttgccagc
    21901 ataatcacca tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga acgatctgcc
    21961 agtttgccgc cgagatagtt accgatagag aatccgacac caatcagcac cagcattgcc
    22021 gtgacgaaca ccggtgttgc gtgggtaata ctttgcagta ccggagagat ataggtgtag
    22081 agagtaaaca ttgcgccagc tcccagtacc gtcgtcagca atgcagacag cacctgcgga
    22141 cgcattaata ccgccagctc ttttttcact tcaggtcgtg cccctgtacc acctttaggt
    22201 aatgagaaga acagacttac cattgaaatc actcccagcc ccgccgttgc cagaaatgac
    22261 atccgccagc cgatggtttc acccaaccag gtcgccgccg gcacgccacc gatatttgcc
    22321 agggttaacc ccataaacat agtggcaact gcgctggcct gtttatgttt tggcaccacg
    22381 cttgcggcca cgactgaacc caaaccaaaa aatgctccgt gattcaggct ggtcaaaatg
    22441 cgtgaaagca tcagggtcat ataatccggc gcgatggcgg aaagtacgtt gccgagcgtg
    22501 aaaattgcca tcaggaaaat caacgcactg cggcgggcac gatgagaaag tagaagcgtc
    22561 atcagcggcg cgcccaccat tacgccaact gcataggcac tgattaacat tccggcagcg
    22621 ggaatcgaga catccacacc gcgcgcaatg acgggcaaca agcccattgg cgagaactcc
    22681 gttgtcccga taccaaacgc gccaatcgcc agcgccagca acggatagtt aattttcatg
    22741 ccttatctcc acctcttcgc gtcattacgc gatattcatt aaagtggcga aagcatgaca
    22801 gcaatcacaa aaaaatgaaa ataacaaaaa gagaaagcac ttttgccatt ttgctaacaa
    22861 acaggaagga gatgcgaggg agaacgcgct ccctcgagag gaaatcagtg cagcgcggca
    22921 gtcaaaccca cggctacgat caaaccgagg acgataatcg ttgttaccag tgaaaattta
    22981 aggtcggtgc tcatcaagtt ttctcctttt ttattaccac acaaaaagtg atattacgca
    23041 tttttacaca ctgtgatgaa aaaatctccc gtcatttata atgataagtg tttttaccac
    23101 ttcccctttt cgtcaagatc ggccaaaatt ccacgcttac actatttgcg tactggccat
    23161 tgaccccttc ctgacgctcc gtgtcgtttt tccggcgtac cgcaacactt ttgttgtgcg
    23221 taaggtgtgt aaaggcaaac gtttaccttg cgattttgca ggagctgaag ttagggtctg
    23281 gagtgaaatg gaatggcaac aataaaagat gtagcgaaac gagcaaacgt ttccactaca
    23341 actgtgtcac acgtgatcaa caaaacacgt ttcgtcgctg aagaaacgcg caacgccgtg
    23401 tgggcagcga ttaaagaatt acactactcc cctagcgcgg tggcgcgtag cctgaaggtt
    23461 aaccacacca agtctatcgg tttgctggcg accagcagcg aagcggccta ttttgccgag
    23521 atcattgaag cagttgaaaa aaattgcttc cagaaaggtt ataccctgat tctgggcaat
    23581 gcgtggaaca atcttgagaa acagcgggct tatctgtcga tgatggcgca aaaacgcgtc
    23641 gatggtctgc tggtgatgtg ttctgagtac ccagagccgt tgctggcgat gctggaagag
    23701 tatcgccata tcccaatggt ggtgatggac tggggtgaag caaaagctga cttcaccgat
    23761 gcggtcattg ataacgcgtt cgaaggcggc tacatggccg ggcgttatct gattgaacgc
    23821 ggtcaccgcg aaatcggcgt tatccccggc ccgctggaac gtaacactgg cgcaggccgc
    23881 cttgtcggtt ttatgaaggc gatggaagaa gcaatgatca aggtgccgga aagctggatt
    23941 gtgcagggtg actttgaacc tgaatccggt tatcgcgcca tgcagcaaat cctgtcgcag
    24001 ccgcatcgcc ctactgccgt cttctgtggt ggcgatatca tggcaatggg cgcactttgt
    24061 gctgctgatg aaatggggct gcgcgtcccg caggatgttt cgctaattgg ttatgataac
    24121 gtgcgcaacg cccgctattt tacgccggcg ctgaccacaa tccatcagcc aaaagattcg
    24181 ctgggtgaaa cagcgttcaa catgctgttg gatcgtatcg tcaacaaacg tgaagaaccg
    24241 cagtccattg aagtgcatcc gcgcttgatt gaacgccgct ccgtggctga cggcccgttc
    24301 cgcgactatc gtcgttaatc acccgttgcg ggagtctctt ccggctcccg cagccactcc
    24361 ttattcagcg tctcactatc gccgagatac tcaagcaacc aggttaacgc aggcgacata
    24421 tcattttgct gccatgtcag acaacatgcc gaatccggaa aggggttttc cagttcaagt
    24481 gctacccact tcccctcatt aagccacggt ttggcgaaat gtgttggcac catccctatg
    24541 cataatcctg ccgagataca ggttgccgat gattcccagt caggcacgac aactcttttt
    24601 tggttatcca gcaaccaggt aatacgttta ggtagcgttc gcgaggtgtc ttcgcgcacc
    24661 aacgacggcc agttgcgcaa cgtatcatcg ctgaacgggc catccatcaa cgccagcggg
    24721 tgatggctgg caacaacgca actccagctt agcatcccca tatcccggaa ggcataacga
    24781 ccgcctaccg gaatcgcgcg tgttgcgcca atcgccagtt ccacgcgccc gtcggaaagc
    24841 gcatcccaga caccgttgaa cacttcctga aagacaagaa gttcgacatc atcaaaatgg
    24901 cgataaaaat caacgatcat ctgccgtgta cgttctggcc tgacaatatt atccactgcg
    24961 atagctaact gaccgcgcca gccgttcgct atctgctgac attgctggcg ggtgatctgc
    25021 atttttttga caacagagcg cccttctttg agaaaccacg ctccagcagc ggtcagctcc
    25081 acatcacggt gccgtcgttc aaagagcggc accgccagcc actcttccag ctgacgcacg
    25141 gtatagctga ccgcagaagg aacgcgatgc agctcctgtg ccgcagcgct aaaactacca
    25201 ttacgcgcta ccgcatcaac aacttcgagt gaatattctg accacatagt ctgcctgcaa
    25261 aatttttgaa accaatcatc aaatattacc gtttcacaac actaatttca ctccctacac
    25321 tttgcggcgg tgtttaattg agagatttag agaatataca tgcaacctgg gaaaagattt
    25381 ttagtctggc tggcgggttt gagcgtactc ggttttctgg caaccgatat gtatctgcct
    25441 gctttcgccg ccatacaggc cgacctgcaa acgcctgcgt ctgctgtcag tgccagcctt
    25501 agtctgttcc ttgccggttt tgccgcagcc cagcttctgt gggggccgct ctccgaccgt
    25561 tatggtcgta aaccggtatt attaatcggt atgacaattt ttgcgttagg tagtctgggg
    25621 atgctgtggg tagaaaacgc cgctacgctg ctggtattgc gttttgtaca ggctgtgggt
    25681 gtctgcgccg cggcggttat ctggcaagcg ttagtgacgg attattatcc ttcacagaaa
    25741 gttaaccgaa tttttgcgac catcataccg ctggtgggtc tatctccggc cctggctcct
    25801 ctgttaggaa gctggctgct ggtccatttt tcctggcagg cgattttcgc caccctgttt
    25861 gccattaccg tggtgctgat tctgcctatt ttctggctca aacccacgac gaaggcccgt
    25921 aacaatagtc aggatggtct gacctttacc gacctgctac gttctaaaac ctatcgcggc
    25981 aacgtgctga tatacgcggc ctgttcagcc agtttttttg catggctgac cggttcaccg
    26041 ttcatcctta gtgaaatggg ctacagcccg gcagttattg gtttaagtta tgtcccgcaa
    26101 actatcgcgt ttctgattgg tggttatggc tgtcgcgccg cgctgcagaa atggcaaggc
    26161 aagcagttat taccgtggtt gctggtgctg tttgctgtca gcgtcattgc gacctgggct
    26221 gcgggcttca ttagccatgt gtcgctggtc gaaatcctga tcccattctg tgtgatggcg
    26281 attgccaatg gcgcgatcta ccctattgtt gtcgcccagg cgctgcgtcc cttcccacac
    26341 gcaactggtc gcgccgcagc gttgcagaac actctacaac tgggtctgtg cttcctcgca
    26401 agtctggtag tttcctggct tattagtatc agcacgccat tgctcaccac caccagcgtg
    26461 atgttatcaa cagtagtgct ggtcgcgctg ggttacatga tgcaacgttg tgaagaagct
    26521 ggctgccaga atcatggcaa tgccgaagtc gctcatagcg aatcacactg atctatatcg
    26581 atatacttat acttaggctg ctaacaaaat tttgttgtat gattgaaatt agcggcctat
    26641 actaatttcg agttgttaaa gctacgataa atattatgtt tttacgggga caggatcgtt
    26701 cccgactcac tatggatggt catttcggca agggttcctc ctttccctct gttctacgtc
    26761 ggattataga ctcgcggttt tttctgcgag atttctcaca aagcccaaaa agcgtctacg
    26821 ctgttttaag gttctgatca ccgaccagtg atggagaaac tatgagttca tcgtgtatag
    26881 aagaagtcag tgtaccggat gacaactggt accgtatcgc caacgaatta cttagccgtg
    26941 ccggtatagc cattaacggt tctgccccgg cggatattcg tgtgaaaaac cccgattttt
    27001 ttaaacgcgt tctgcaagaa ggctctttgg ggttaggcga aagttatatg gatggctggt
    27061 gggaatgtga ccgactggat atgtttttta gcaaagtctt acgcgcaggt ctcgagaacc
    27121 aactccccca tcatttcaaa gacacgctgc gtattgccgg cgctcgtctc ttcaatctgc
    27181 agagtaaaaa acgtgcctgg atagtcggca aggagcatta cgatttgggt aatgacttgt
    27241 tcagccgcat gcttgatccc ttcatgcaat attcctgcgc ttactggaaa gatgccgata
    27301 atctggaatc tgcccagcag gcgaagctca aaatgatttg tgaaaaattg cagttaaaac
    27361 ctgggatgcg cgtactggat attggctgcg gctggggcgg actggcacac tacatggcat
    27421 ctaattatga cgtaagcgtg gtgggcgtca ccatttctgc cgaacagcaa aaaatggctc
    27481 aggaacgctg tgaaggcctg gatgtcacca ttttgctgca agattatcgt gacctgaacg
    27541 accagtttga tcgtattgtt tctgtgggga tgttcgagca cgtcggaccg aaaaattacg
    27601 atacctattt tgcggtggtg gatcgtaatt tgaaaccgga aggcatattc ctgctccata
    27661 ctatcggttc gaaaaaaacc gatctgaatg ttgatccctg gattaataaa tatatttttc
    27721 cgaacggttg cctgccctct gtacgccaga ttgctcagtc cagcgaaccc cactttgtga
    27781 tggaagactg gcataacttc ggtgctgatt acgatactac gttgatggcg tggtatgaac
    27841 gattcctcgc cgcatggcca gaaattgcgg ataactacag tgaacgcttt aaacggatgt
    27901 ttacctatta tctgaatgcc tgcgcaggtg ctttccgcgc ccgtgatatt cagctctggc
    27961 aggtcgtgtt ctcacgcggt gttgaaaacg gccttcgagt cgctcgctaa aggctattct
    28021 atcgccccct ctccgggggc gatttcagat caggcttctg tacctggttg attcatggca
    28081 ttttctcgtg ccgccagcac acgttctacc gtatctacca ctgcctgagt ttgtggatca
    28141 atttcaatgt tgacgcgtgc gccgagtttt ttcttcccaa gagtcgtgcg ttccagagtt
    28201 tccggaatta aatggacgca aaaacgcgtt ggcgtgactt cgccgacggt caggctaata
    28261 ccgtcgatgc caataaatcc tttgtacagg atatatttca tcaactgact atcctggact
    28321 ttaaaccaga tctggcgatt attttccgag gttaatattt tcgccacttc agcggtggtc
    28381 ataatatgac ctgacattaa gtgtccgcca atttcatcgc tgaattttgc cgcacgctca
    28441 acgtttaccc aatcccccac ttttaaatcg ccaagattgg taatgcgtaa cgtttctttc
    28501 atcaggtcaa aactgacatg gttgccgtta atttccgtca cggtcaggca gcaaccgtta
    28561 tgcgccacgg aagcacctgt ttccaggccg tccagcatgt ggtcgggtaa ctccaccaca
    28621 tgcgtacgaa aatttggttt ctcgtcaatc gacaccagtt ttgcggtgcc ctgtacaatc
    28681 cccgtaaaca tacttacaac tcctgaaatc agttaagaca ttctgttcag cacaatagca
    28741 ggtggaaaac gcccttacca gtgaaggggt aagaatgact attttttcac tggagaatta
    28801 ataaatcctc gctacaatag actgaatttc ccctgcttct tctttttgct gcccattcag
    28861 gcggcttttt agtctctcat ataactacaa ataaaaggtg ttcacgtgca gaagtatatc
    28921 agtgaagcgc gtctgttatt agcattagca atcccggtga ttctcgcgca aatcgcccaa
    28981 actgcgatgg gttttgtcga taccgtgatg gcgggcggct atagtgccac cgacatggcg
    29041 gcggtcgcta tcggtacttc tatctggctt ccggcgatcc tctttggtca cggactgctg
    29101 ctggcattaa cgccggttat cgcgcaatta aatggttccg gtcgacgtga gcgcattgcg
    29161 catcaggtgc gacaaggttt ctggctggca ggttttgttt ccgttctcat tatgctggtg
    29221 ctgtggaatg caggttacat tatccgctcc atggaaaaca tcgatccggc tctggcggac
    29281 aaagccgtgg gttatctgcg tgcgttgttg tggggcgcgc caggctatct tttcttccag
    29341 gttgcccgta accagtgtga aggtctggca aaaaccaagc cgggtatggt aatgggcttt
    29401 atcggcctgc tggtgaacat tccggtgaac tatatcttta tttatggtca tttcggtatg
    29461 cctgagctcg gtggcgttgg ttgtggcgtg gctactgcgg cggtgtattg ggttatgttc
    29521 cttgccatgg tttcttacat taaacgcgcc cgctccatgc gcgatatccg taacaaaaaa
    29581 ggcaccgcaa aacccgatcc tgcggttatg aaacgactga ttcaactcgg tttgccgatt
    29641 gcgctggcac tattctttga agtgacactg tttgccgtcg tggctctgtt agtgtctccg
    29701 ctcggtattg ttgatgtcgc aggacaccag attgccctga actttagttc actaatgttc
    29761 gtgcttccaa tgtcgctggc ggcagcggta actatccgcg taggttatcg tctgggtcag
    29821 ggctcaacgc tggatgcgca aaccgctgct cggaccgggc ttatggtggg tgtctgtatg
    29881 gcaaccctga cggccatttt cacggtttca ctgcgggagc aaatcgccct gttgtacaac
    29941 gacaatcccg aggttgtaac gctggctgcg catttgatgt tgctggcggc ggtatatcag
    30001 atttctgact caatccaggt gattggcagt gggattttgc gtggttataa agatacgcgt
    30061 tccattttct atatcacctt tacagcttac tgggtgctgg gcttgccaag cggctatatt
    30121 ctggcactga ccgatctggt cgttgaacct atggggccag caggcttctg gataggcttt
    30181 attatcggcc tgacatcggc agccattatg atgatgctgc gtatgcggtt cctgcaacgt
    30241 atgccgtcag ccatcattct gcaacgagcg tcccgctaat aaagacaagg cgcaaccttc
    30301 acgggttgcg cctgtgtttt tacgcaggct ggagcgttgc gccaatcccg tcttcgtctg
    30361 gctgtaattt cagagcgtta cacagagttt gccgactggc atctgttatc tcaacgcgta
    30421 tatccccgtt aacttcccct ttcacattga tgaagtcagg tgaaatttct ttcgcactgg
    30481 tttgcaggca aatcgctccc tctccgcgca ggcgtttcac tttcagttgc acatgcgttg
    30541 ccgcaccagt gatcagccac agttttccgc cattcagatt gatgttacca acaatgaaag
    30601 tgacgctgtc accttttacc gtactgccgt cgagaatcaa cacaccgccc tgctgtacag
    30661 taatcgttga ttgttgcagc tctgcgccat aaccaataat cattgaagcg ccgtccagta
    30721 atgcaacact cccggtcagt tcaaccggtg cgcgatcttc ctctacgaca aaattttcgt
    30781 ccgcgctggt gggtgacaac aggatcactg ttccaccttg tattaccgca ttttccagcg
    30841 taccggcatg cacttcagcc cgccccccca cattcaccga caggttttgc gtcttaccgg
    30901 aactgtagag ctgaaggcca tccgtcccca gacgataaat ggatccattc tcaacaatag
    30961 tgtcagagtc ttcaccaccg agattattca tggtgccacc agcaagcaac gtatcactgg
    31021 ctttcccggc cagcataaaa acgccgccct gattaatctc acaattgctg gcttctccac
    31081 ttggatacac caacaattca ccaccttcat ctaccacgac cgctgaggtt gtcccattaa
    31141 ccaacaggcc gccctcttga tcgagaatga ttttttccgc gcgatgtcct tccagtacac
    31201 gcaggttacc gccattttcc agcaacaaac cgcaggcata accctgatca acgctgaatt
    31261 caccttcggg atggcggcca ttaaccgtag cgagcgtaga gagactaatt gcgcctccct
    31321 cggactgaac aatatctgtg gcaatggcac cgtcgctgac cgatagcgtg ccattgttac
    31381 cgtactgtgt gcatttggcg atcccaccgg ttgccacttt caaccagcca gcccccgtga
    31441 ccacggtatt ttcggctctt ccgccagctt caacaatcaa ctcgccttca gtaagttgcg
    31501 tcgcgccaat gacctcgccg gtcttaacaa tttgcacatt cccgtcgctc atcaagtttt
    31561 tcgcatcaga tcccatgatt tattcctttg ctgcatctgt gtgcctttat tgctacctaa
    31621 gtgtaaaggc tacggaggat ttatccacga cagatttgag atggtggcaa acaattctgt
    31681 ttaaactctg atacacgaat tattgggttg tatcagatgt aaatgcgatc ctgaataaaa
    31741 atcgcccttg caaatcaaca aaatatagcc aatttgctta aaagctctgc aaacggtcga
    31801 aatagcgcag aaaattacgt tttgcctctt gccacctttc cactctgccg ctaatatttg
    31861 tccccgttgt cacctacaac gttgcgttca tagctcagtt ggttagagca ccaccttgac
    31921 atggtggggg tcgttggttc gagtccaatt gaacgcacca tcctgcgtcc gtagctcagt
    31981 tggttagagc accaccttga catggtgggg gtcggtggtt cgagtccact cggacgcacc
    32041 agattttctt aatctggtct tttccttttt ccctctgttt attctctgta tccaatacgt
    32101 taaaagattt acactgtctt cgtatgcgtt atcagaagga gaatcgctat ggcaactttg
    32161 ttacaacttc attttgcttt taatggccca tttggcgacg caatggctga gcagcttaaa
    32221 ccacttgctg agtcgattaa tcaggaacct ggttttctgt ggaaggtatg gacagaaagt
    32281 gaaaagaacc acgaagccgg tgggatctac cttttcactg atgaaaaaag cgcccttgcc
    32341 tatctggaaa aacatactgc ccgactgaaa aatctcggcg ttgaggaagt tgtcgccaaa
    32401 gttttcgatg tcaatgagcc acttagtcaa atcaatcagg caaaactcgc ctgacagaat
    32461 ttaatcaagg gcggttagcg cccttttcat ccctgtctga aatttctcaa attctaaaaa
    32521 tctcaaccaa acttatctga taacactaaa ttcgaaagaa tgcgtacagg taagtaacaa
    32581 tgaaaaaaat tgctattgtg ggtgccgggc ctacggggat ctacacctta ttctcgcttc
    32641 tacagcaaca aactccactt tctatttcta tcttcgagca ggctgacgag gccggtgtcg
    32701 ggatgccata cagtgatgag gaaaactcaa aaatgatgct ggcaaatatt gccagtattg
    32761 aaataccgcc gattaattgt acgtatctcg aatggctaca aaagcaagaa gccagccatc
    32821 tccagcgtta tggcgttaaa aaagaaacct tgcacgatcg tcagttttta ccgcgaattc
    32881 tgctgggcga atatttccgc gatcaatttt tacgattagt agaccaggca cgacagcaaa
    32941 aatttgcagt ggctgtttat gaatcatgcc aggttaccga tctgcaaatt acaaatgctg
    33001 gcgtcatgct cgctacaaat caggatttac ccagcgagac gtttgattta gcggtgattg
    33061 ccacgggtca cgtctggcct gatgaagaag aagcaaccag aacgtatttt cccagcccgt
    33121 ggtcaggcct gatggaagca aaggtcgatg cgtgtaacgt gggtattatg ggaacatcct
    33181 tgagcggact ggatgcggca atggcagtgg ctattcagca tggttcgttc attgaagatg
    33241 ataaacaaca cgtcgttttt caccgcgata acgcaagtga aaagctaaat atcacgttga
    33301 tgtcgcgcac gggtatttta cccgaagccg atttctattg ccctattccc tacgagccct
    33361 tacacatcgt caccgatcag gcattaaatg ctgagattca aaaaggcgaa tatggccttt
    33421 tggatcgggt atttagattg atagtagagg aaatcaagtt tgctgatcca gactggagcc
    33481 aacgcatagc cttagagagc ctgaatgtcg attcctttgc tcaagcctgg tttgccgagc
    33541 gcaaacaacg cgacccattt gactgggcag aaaaaaatct ccaggaagtc gaacgcaata
    33601 aacgagaaaa acatactgtt ccctggcgtt atgtcattct gcgcctgcat gaagccgtac
    33661 aggaaattgt tccacatctg aatgaacacg accataaacg gttcagtaaa ggccttgccc
    33721 gggttttcat cgataattat gcggcaatcc cttcagagtc tattcgtcgg ctgctggcct
    33781 tacgtgaagc ggggatcatt catattctcg ccctcggtga agactacaaa atggaaatta
    33841 atgagtcgcg caccgtcctg aaaacggaag acaacagcta ctcgtttgac gtttttattg
    33901 atgcccgcgg acagcgtccg cttaaagtga aagatattcc tttccctggg ctacgcgagc
    33961 aattacagaa aacaggggat gaaatccctg atgttggcga agattatacg ttacagcaac
    34021 ccgaagatat tcgtggacgc gtagcgttcg gcgcgttgcc ctggttgatg cacgaccagc
    34081 ctttcgttca gggacttacg gcatgtgcag aaattggtga ggcgatggct cgggcggtcg
    34141 taaagcctgc atcccgtgct cgtcggcgtc tttcgtttga ttaaaggtaa agctatcagc
    34201 actcaacaaa ctgatgctgt tcattaagac gatagcggac tccggttcga atattgtttt
    34261 caccttcaat ggcgacggcg aagcgaactc tttcgccgtc atgatatgcc agtgcggcgc
    34321 ttccccctgg cccgagaata attgagtcga ccacgcccgt gctcactacc gttgaatttt
    34381 caccgctggc aatgatgtgt acattgtcgc cgctgttggc aattctggcg tttgcgccaa
    34441 agctggcgat ttgcaccagg tcaccattac tggcgacatg acaccgttca cccaatgtac
    34501 aaacgcgtac tcgcattccg gtattcgcaa tccgactact atctccagcg ctgcttatcc
    34561 gcgaagaatt accggttaca gcaacccgtg cgcgttcacc ggagctgcca atgtgactgt
    34621 taaagccgac acttccaatc cgcacgctat aacccatact ggcaatttgc gcggcatacc
    34681 cagcggttac gattcgggcg ttatcctcac aacagccgat gcgagcatga tcgtcgctgc
    34741 gggcaaactg actgcaaaat ggtatctctc cggggtctgt ggccagtttt tgcatagagg
    34801 aaacttcctg atgcgcgaag ttttcatccg ccagccattt tttccacgca tactcaacca
    34861 gactctccgc ccagtccgta tatccttcct caatcagtgc atgatgaata tcggcataac
    34921 taccgccgcg tgggaaatgg cgcaggaacc agcgatacat caccgctccg atacgccatt
    34981 ctcgcaggtc tgctcgtgta atgatcattc gttagtgccg gtgatagcca tcgaccatgc
    35041 ttttaaaggt ttcgtgtggc gtacgccccg tggtgcaaag ataaagatgt ccgaagataa
    35101 aaaagaggct tataaatgcc agagcaaaat gcgcctgcaa taaccagtat cttacgccag
    35161 gaaacacatc tcccacggct tgcggataga gacacaacag ccccgtcaat agtaacaacg
    35221 gcagcaatcc acacatgaca ccaacatagg cgacctgctg taagggatta aatttagact
    35281 gtgttgttgc cgggaaagga tgttcttccc cctgcataat gccaaacaaa taaaatcgtg
    35341 tttgttttgc cgctcgttcc agccacccct gacgacgaat gcgatagtgg tgaccattat
    35401 ccccaacggc attgatcagc acaaagccga gccagcatgc cagtaacaaa aatccgcaaa
    35461 cttcatgcac cgcaaccaga cttttaaccg cagttgcgcc caccatcgca aagtgattta
    35521 tcagcccact tgccagcaac aatacaaaga gtaacgcatt cgaccagtgc cataatctga
    35581 ccgctttgct gtaaagataa accttctcac catgatcggt ggctgatttc ttcacgccac
    35641 gagcacgaag caaggcgtgc agccccaaca ccaaccacat cccaaccagc aatactccgg
    35701 caatgatcaa ccacaccggc caaaattcgg gagtgaactg tggcacatag ttcgctaact
    35761 ggctctgaaa ctgttcagca tgttgcgacg ggttcataca ttttcctttt taatcaaatg
    35821 ttgaccgaac cggcgataca gatgcggttt cccggcacca agtagctgat attgatagta
    35881 tttattttcc tgcaaccacg cctgaatttc tgggctatct tcgcgaccaa agatcaatgc
    35941 gtgttccgga caggcgctga cgcaaatggg cggaaagcct tttgccagtc gggactcagc
    36001 gcagaaatca catttatctg ccactttagt gacagggtta aggtagcgaa cctggtacgg
    36061 acaagcgcca atgcaataac tgcagccgat gcactgcgat ttttctaccc gcacgatccc
    36121 ctgctcatca cgccacgacg cccctgtcgg gcatacgtcg atgcacggag catcttcgca
    36181 gtgctgacat gactggcgaa aaaagtgata ctgcgtttcg ttgtcgttat cggtgactgg
    36241 aatgtgtgct atcgataaac ggcttccctg agcggggacg tggttagttt tacggcatgc
    36301 gcgggcgcaa atattacagc cattgcaccg tgactcgtca tgaatcatgg cataccgaac
    36361 ttccttttct tgcctcgtgt tcgctaacag agaagacgca gaaccagtaa aaaagattac
    36421 tgttcccatc cccagaacaa atttgcgtcg agtgaaggac atctgattat cccctaatgc
    36481 agtggctgca actgtccacc ctatccatga catctccctg aaatttcagg ggttctgaaa
    36541 ctgctctacc tcgaaccatt cgcataagcc ataagccttg ccataggtca taaatcgctc
    36601 gctaaacaag tacaacaccg ccgtcgatga ctccacctgc cgtatatcgg catattcagg
    36661 tgcaacatcc atcgcggcga tcgcggcttc aatctgcgct tcctggaagt aatatggcgc
    36721 ttgcatcagc atggcgactt tgtaaggacg aggatacgtc tggcactcaa aacgaacggc
    36781 gtgtgcaatg gcgcgacaaa tgtcctgctc cacaacttgc aaagacatcg ccgcgtagtt
    36841 ttcactcatg gcctgcgttg agtaatagta atcatcctga cttcccgtca tacaagcgat
    36901 atccgcgtag tctccgttct gcttaatgct ttccagcaaa actgacagtt cctcttctgc
    36961 aaccgaatag ggtggcgaga gaaacacggt gcgcgccacc agctgcccag ccgcagaatg
    37021 ctggcgaatg aactcagcca ggatttcagc aggttccggt tcggttaccg tatcaacggg
    37081 gtgacgcatc ccctgcaacc attgccgctt cgcctcgtca acttcgctga ctttcgctaa
    37141 cggcaattca tcctgatggt tcatcttacc cattccttac gcaggcagta gattgtgtgc
    37201 tgccagatcg gcggcaatat cttccagccc cagacgctgc aatgtttcgc gggttggaca
    37261 accaagctgt gggtcccagc ccatttcttt gtagaacatc gtgagcgaag catgcatatc
    37321 gtcacgatcc attttgtcag taccttcagt aaagaccggg atctgcggat ccttgtcgaa
    37381 aacccaggaa cagataagat cgtgttcatt acgcatatct ttggtttgca tcagttttac
    37441 cgtgtaggca cgatgcaacg taaagatacg ctctgcagct aaatctaatt tttcctgagt
    37501 catctcttcg ccggtgatcg ctttgaagaa tttggcttca agcgccagat cgccccggta
    37561 attacggctt ttcagcggcg aaacggtcat tggccagacc cagttgcaca gggtgacggc
    37621 gttatgcaaa cagacccgca acagcgacca ctttgcatat ttaatttttg cgtcattgat
    37681 tggcgtgtag tttttggttt catcgtaagc atcttcagaa ccaaaaagtt ctttcgccac
    37741 ttcacgttgc agtttcaatg gcaagccgga accaataaag ttgatatggg tatgcgtcat
    37801 gcagtcacgg ttgaacatac agttaacaat ggaaccgacc tgcgctgacg cttcattggc
    37861 atggtgaacc ggatagccaa acggcgacca gagtttattt ttcgcgtagc cccagtactc
    37921 ttcacccaaa ttccagcgtt ctgcgatggc atatgaacca tcagccaggt gactcagctc
    37981 acccacacga tgcgccagac ggtagtaaaa atctttaatg aagttaacgt cacccgcttc
    38041 cagttgatcc cagcgaattt ccgcatactc ttcagcaggc agaacacgct tgaacacacc
    38101 tttgctgtaa caataggtaa agtcgcgatg caactgcccg tagttacacc ataggccgta
    38161 gtcatcgaac agattcagac ccaccaggtt accaatcaca cgaccatcgt ctttatcttc
    38221 aaaatctttc ggaccgttcg ggaagatggt ggtatggaca aagttggcta cacaagtgtt
    38281 accacctgtg ctgggcacgc caaactcttt cacccgagga atattcattt gggtcataca
    38341 acggatcggg caagagtggc agccactcat ttttactgtg tatttctctg ccgccggacc
    38401 taagtcaaaa acggatttat aggtacgaaa gccgaccgtg ttctgattgc ctggcggaat
    38461 ttcccccgtt tcaatcggac caccttcagc cgcgccccaa aacagccctt tacgtgctgt
    38521 ccaacgtgac ttgggatctg aatactctgc ccacgattgc ggcgtacttg gcacgacatg
    38581 gttgttattc gcaccaataa gttcagtcat catgtaatcg ttgagacgct tcatctcctg
    38641 acgatcggca atgttgaccc ctttcgtccc ttcaaccgca atcgctttca ggtttttcga
    38701 acccattatt gcgccagttc ccgcaccgcc gctgtggtta cggctattca acatgccaga
    38761 gagaggaaca aggttttccc cagcctgacc aatagccgcc acacaggttt ccggactggt
    38821 caatcgacaa atttcttccg tcgttgcgcg cgtccctttt ccccataaga aatcggcttt
    38881 ttccaggcta actttgtcat ctttaatctt cagccatacc ggtgatttcg ccttcccttc
    38941 gataataatg acgtcgtatc cagcgaattt catttgcgct gcaaaaaagc cacccatatg
    39001 ggcatcgacg actaaatttc ctttggtaaa agtagaaagt gaggtgatat ttacgcgaga
    39061 actacagggg gcaccagatc cagttaatgg gccggtagca aagactaatt tattcgcttc
    39121 atcgaaaggt ttcgtgcctg gcggtacttc gtcatacata attttgtagc cgaagcccat
    39181 gccaccgaca aaacttttaa acttactgga atcttcgagg gtaatatttc ctgtcgtgag
    39241 attgactctt aatatattac ctgtccaacc gttagccatg attttttcct ttgcaagatt
    39301 cacacagtaa tatctttcca ctcgataatt tttaacgccc ctgttgggca ggcgtttgcg
    39361 cattcaccgc ataacacaca tttcgaggat tttttacttt cggtatttac ggtggccatc
    39421 atccacgggc atgccgtagt acaggcgcta cagccaatac agcgtttatg atcgacggta
    39481 atacagcctt ctttctgctg ccaggtaatc gcaccaatcg ggcagacgtt catgcattgc
    39541 ggctctttgc attgacggca ggtgtccgcg gtatagttga gatcgccata caggccgccg
    39601 ccagagccaa ccccgttgtc gccaaagaaa taattgcgat ggattttgat acgggagaag
    39661 aatgtcccta ctgagccatc gttgaagttg gtacaagaga tttcacagcg gtggcaaccc
    39721 gtgcatcgcg ctcgctgggt caccagcacc cctttgggtg tatttatcaa ccctactgtg
    39781 ccgctatcaa tatcttcctg tttgcaaccc aaaagtgaca gtaacgcagg ggcaattgtt
    39841 agaccagcaa ggccttttcc tgatatacgc aagaattcta atcgcgttaa gccaatatct
    39901 aatagtggac gatcaaccgg gttcatttat ttcgcatcct cttcgcaaac acagtgctga
    39961 caattcagca gcctgatatt aagcattcaa ctaatacgct gctgactctg tccccaacac
    40021 cagatatttt ccctaacccg atagtgtatg agaaaagatt taacgccgat aaaccaatcc
    40081 tttatggtta ggagaaaaaa taatgtgatt attctgagcc cttaacattg atcgttatca
    40141 attaaaatta caaagcaaga aattataagt gaactgatat ttattatcaa ttgaaataaa
    40201 tttaacttaa gcgacaatta tttgtgacgt tcattgcaga aaataaaaat aatattatcg
    40261 ttatagatta aaatacatac cgcaattcgt ataatttcag cagttgttat ttagaatgat
    40321 taactggacc gcaactgaag caagaatatc aggatgaagc ttaccgcctc atcctgaatg
    40381 gtcattttac cagtgcgtta atgacggttt ccatcgcctg cttttccagc tcactgcgct
    40441 gaatgcgttt attggctaat gcagtacgta atacgcctgc gataacaata tgctttggtt
    40501 cctgccccag atcgcgcatt tccagcacaa ctttaccgac aactctgcac atttctcggt
    40561 acagttcatc ctcttttgtc cgatttccca ttctttatac ctatttatca tgccaactat
    40621 cagcatatat caatctaacc aattaaacaa aaatcaaaca aaatcagaca aataacgcga
    40681 taaattattt taactgtagc aattgagcga tgatatattt atacaccgga tgaactttca
    40741 cttatcctca cactgacaac ttcggcacca gacgttgcgc aaacagcgaa gtttttgcgt
    40801 aaccttttcc ctggaacgtt aaatctttga taacaattta ttgtctaaca agttgtatat
    40861 tttttgaaac gctgtttttg ttttcctttt ggattaattt cagcgtataa tgcgcgccaa
    40921 ttgactcttg aatggtttca gcactttgga ctgtagaact caacgactca aaaacaggca
    40981 ctcacgttgg gctgagacac aagcacacat tcctctgcac gctttttcga tgtcacctat
    41041 ccttagagca aggcaccacc actttcgtaa taccggattc gctttccggc agtgcgccca
    41101 gaaagcaagt ttctcccatc cttctcaact taaagactaa gactgtcatg aaaaagacca
    41161 aaattgtttg caccatcgga ccgaaaaccg aatctgaaga gatgttagct aaaatgctgg
    41221 acgctggcat gaacgttatg cgtctgaact tctctcatgg tgactatgca gaacacggtc
    41281 agcgcattca gaatctgcgc aacgtgatga gcaaaactgg taaaaccgcc gctatcctgc
    41341 ttgataccaa aggtccggaa atccgcacca tgaaactgga aggcggtaac gacgtttctc
    41401 tgaaagcggg tcagaccttt actttcacca ctgataaatc tgttatcggc aacagcgaaa
    41461 tggttgcggt aacgtatgaa ggtttcacta ctgacctgtc tgttggcaac accgtactgg
    41521 ttgacgatgg tctgatcggt atggaagtta ctgccattga aggtaacaaa gttatctgta
    41581 aagtgctgaa caacggcgac ctgggcgaaa acaaaggtgt gaacctgcct ggcgtttcca
    41641 ttgccctgcc agcactggct gaaaaagaca aacaggacct gatctttggt tgcgaacaag
    41701 gcgtagactt tgttgctgct tcctttattc gtaagcgttc tgacgttatc gaaatccgtg
    41761 agcacctgaa agcgcacggc ggcgaaaaca tccacatcat ctccaaaatc gaaaaccagg
    41821 aaggcctcaa caacttcgac gaaatcctcg aagcctctga cggcatcatg gttgcgcgtg
    41881 gcgacctggg tgtagaaatt ccggtagaag aagttatctt cgcccagaag atgatgatcg
    41941 aaaaatgtat ccgtgcacgt aaagtcgtta tcactgcgac ccagatgctg gattccatga
    42001 tcaaaaaccc acgcccgact cgcgcagaag ccggtgacgt tgcaaacgcc atcctcgacg
    42061 gtactgacgc agtgatgctg tctggtgaat ccgcaaaagg taaatacccg ctggaagcgg
    42121 tttctatcat ggcgaccatc tgcgaacgta ccgaccgcgt gatgaacagc cgtctcgagt
    42181 tcaacaatga caaccgtaaa ctgcgcatta ccgaagcggt atgccgtggt gccgttgaaa
    42241 ctgctgaaaa actggatgct ccgctgatcg tggttgctac ccagggcggt aaatctgctc
    42301 gcgcagtacg taaatacttc ccggatgcca ccatactggc actgaccacc aacgaaaaaa
    42361 cggctcatca gttggtactg agcaaaggcg ttgtgccgca gctggttaaa gagatcactt
    42421 ctactgatga tttctaccgt ctgggtaaag aactggctct gcagagcggt ctggcacaca
    42481 aaggtgacgt cgtagttatg gtttctggtg cactggtacc gagcggcact actaacaccg
    42541 catctgttca cgtcctgtaa tattgctttt gtgaattaat ttgtatatcg aagcgccctg
    42601 atgggcgctt ttttatttaa tcgataacca gaagcaataa aaaatcaaat cggatttcac
    42661 tatataatct cactttatct aagatgaatc cgatggaagc atcctgtttt ctctcagttt
    42721 ttttatctaa aacccagcgt tcgatgcttc tttgagcgaa cgatcaaaaa taagtgcctt
    42781 cccatcaaaa aaatattctc aacataaaaa actttgtgta atacttgtaa cgctacatgg
    42841 agattaactc aatctagagg gtattaataa tgaaagctac taaactggta ctgggcgcgg
    42901 taatcctggg ttctactctg ctggcaggtt gctccagcaa cgctaaaatc gatcagctgt
    42961 cttctgacgt tcagactctg aacgctaaag ttgaccagct gagcaacgac gtgaacgcaa
    43021 tgcgttccga cgttcaggct gctaaagatg acgcagctcg tgctaaccag cgtctggaca
    43081 acatggctac taaataccgc aagtaatagt acctgtgaag tgaaaaatgg cgcacattgt
    43141 gcgccatttt tttgtctgct atttaccact actgcgtcgc gcgtaacata ttcccttgct
    43201 ctggttcacc attctgcgct gactctactg aaggcgcatt gctggctgcg ggagttgctc
    43261 cactgctcac cgaaaccgga taccctgccc gacgatacaa cgctttatcg actaacttct
    43321 gatctacagc cttattgtct ttaaattgcg taaagcctgc tggcagtgtg tatggcattg
    43381 tctgaacgtt ttgctgttct tctgctgata gtggtcgatg tacttcaaca taacgcatcc
    43441 cgttaggttc cacggaatat ttcaccggtt cgttgatcac tttcaccggc gttcccgtcc
    43501 gcacgctgga gaacaaggct ttaatatccg gtgcattcat gcgaatacac cctgaactga
    43561 cgcgcaaacc aacgctgtcc ggcgcactgg tgccatgaat gaggtattcg ccattaccat
    43621 gcgcgaggcg cagtgcgtaa cgtcctagcg ggttatttgg tccggcagga acgactggcg
    43681 gtaatttaat gccacgctcc agcgaacgct gacgaatgcc tgccgtaggc gtccaggttg
    43741 ggttagggat tttctgccca acacgcgttt ccatcaccgg cgtttccagc ccctgcaatc
    43801 caatacctat tggataaacc tgcacaatat tttctcccgg cggataataa taaaggcgca
    43861 gctctgcaag gttaacgata atcccctgac gcggtgcatc aggtaataac agttgtgaag
    43921 gaatagttat cgtcgtacca ggttttggca ccgggacgat agtgttattg gcttcaagga
    43981 tcaacattgc cgcagtatca aaacgtcggg caatagcctg aaggttttta tccccttctt
    44041 gcaccgtata cgtttgattt tgcccaacca gtcggcttcc ggttggtggt agcggataat
    44101 caaccgccca ggcagcctgg atggcgctaa aagcgccgat aagcgtgagt gtaagcaaag
    44161 acgcgcgttt cattgtaaac ctcctgtatt tgccggagac tcacgctgaa acgtcggatg
    44221 gcgcttatgt tcacctgaaa ccaaaacact cctgtgcagg tcagtgtaaa cattgaccat
    44281 ccggcaatgt gagccaaccg gatgaaagct gtccctttag tttagctaag tgcagcggct
    44341 ttggcgcgaa ttgcgcgaat catcgcttcc agaccttgtg aacgagatgg ggtgagatgt
    44401 tgggtgagtg ccattttttc aaaccacgga cgcacatcga aactgacaat atcctgcggc
    44461 gtcatctgat cgtagagaat aaagactacc gcaataagcc ctttcacaat cgccgcatcg
    44521 ctgtcgccct gcaattcaat aattccgtgc gcattctggc gcatgacaat ccacacctga
    44581 ctctggcagc cctgaatgct attttgtggg cttcggtctt cgtcgcgtaa ttcagtcaga
    44641 cgctggccca gctcaataat gtagagatat ttctcttccc agttggcgca gcgtaaaaaa
    44701 ttacgcagca atttttcttt atccggcaat aaagccatag tgcctccctg ttatcccagc
    44761 aaacggtgaa tacgttgcag gccggtcacc agacgatcca cttcttcatg ggtgttatac
    44821 atggccagcg acgcccgaca catcgcaggg acgttgtaat aggccattaa tggcatagcg
    44881 cagtgatgtc cggtacgcac agcaatgccg taattatcga gaaaactgcc aacatcatag
    44941 gcgtggtgtt taccgagatt aaaagcaata acgccaagcc tgttttgtgg gccatagaga
    45001 gtgagatccg gtacagattc cagctgtgat agcgcataat gcatcagatt ctgttcatac
    45061 tcggctatgt tattaagccc cagcgccgaa acatactcca gcgccgcgcc aagaccaata
    45121 atgcccccgg tattgggtgt accggcttca aaccgccatg gtgctttggt ccaggtagtg
    45181 ccttcactca ggctgacggt ggcgatcata gaaccgcccc cttcccacgg cggcatctcc
    45241 tgcaacaagg cttctttcac ataaagaatg ccaattccgg tggggccata cagtttatgc
    45301 ccggagaaca cgtaaaagtc gcaatccagc gcctgaacat ccaccggatg atgcatcacc
    45361 gcctgagcgc catccaccag cacttttgcg ccatgctggt gcgcaagcgt gattatttcc
    45421 gccagcggat tttctgttcc cagcacgttg gagacatgag taattgccag caggcgagtt
    45481 ttctcatcaa acagcgtagg cagcgtctcc agttgcagcg taccgtcggg gttgagaggg
    45541 atcacacgca gctctgcgcc aacgcgtgcg caaagcatct gccagggaac aatgttagcg
    45601 tggtgctcca tctgactgat gatgatgtta tcgcccgccc gcacgttgct gttgccccaa
    45661 ctattggcga ccagattgat cccttccgtc gtgccgcgga cgaacaccag ctcttccgcc
    45721 gaacgggcat taataaacag cgatgcccgc ttgcgcacgt tctccatttt ctcggtcgcc
    45781 tgggcgctta aggtatgaat accgcgatgc accgccgcgt agccgtgacg ataaaactca
    45841 gcctcggcgt caatcacctg gcttggtttc tgcgcactgg cggcgctgtc gagataagcc
    45901 agcggcaaac cgttcacctc acgagaaagc accggaaagt cggcccgcac tttgtcgacg
    45961 gaaaaagtca tcttgcacct cctggcagcc gttgaccgat tcgggccagc acctgctgtt
    46021 taagcccctc atcacgcagt gcttccgtca gttcggcagc aaaggcgtaa atgatcatct
    46081 gctgggcatc ctgctgattg atcccgcgcg agcgcagata gaacatctgt tcatcatcaa
    46141 tacgccccac cgtcgcgccg tggctgcatt tcacatcatc tgcatagatt tccagctgcg
    46201 gttttgtatc cacttccgcc attttgccca tcagcagatt attgttggtc atctgaccat
    46261 ccgttttgat ggcgtgctgc gcgacgttga tcaaaccgtt aaataccgcg cggcctttgt
    46321 cgctgacgat agttttgtgc aactgtcggc tgttacaaaa acctttattg tgttccagcc
    46381 aggtacgggt atcacacacc tcgtttttca ccggcatcgc caggctattg atccgcagcg
    46441 tgctgttttc gccattgagt tgcgtactgg tgttgtgtcg taacactgcg ccacccagca
    46501 ggaaactatg gctaaatgcg gtggcatcct cagccagcaa caaatcgtta tgagcaaagt
    46561 ggtgactgag cgggttttca aacgccagct tgatatgctg caagtgggcg ttcgctgcga
    46621 cattgatagt gaaccgtgcc ccggtaaaat gacgagcatc attaaggctg acaaaatgtt
    46681 cgatcaccgt tgcttcggca ccttccgcca gatccagatg atgccggtaa tgggcagtgt
    46741 tcacctcttc acctgccacg ccctgggtga tatgcattaa cagcaatggc tttgccggcc
    46801 gttgaccgcg cttcacggcg atatgcgtca cgctttgtgc caggctttcc gtcaaatgca
    46861 gaaacacttc cgcctgaata gcgtcgggta acccctgacg gtcgtcgtta atgctcactt
    46921 catatccgct gccttcaatt gcatcgctca gcgcgggcac gtaacgtcca tcgacaaaca
    46981 ccagccgcac ggcgtctaac gttaacgcta aggcatcacg ctgctgtggg gatatatctc
    47041 ccgcaatgct gacaaactga ctattggtca gcccttccag cggcgtatat ttccagtttt
    47101 catgtttacg tgtcggcagt ccggtacgca gcaattgttg taaatgctgc tgtgcttgcg
    47161 gtgagcgttt tgtcccttca gcttcaaaca agtgatgcca ctgttgcagc gcattactgc
    47221 tgttcggtaa gccagccata accctgctcc tccagttgtt tgaccaacgt gaaatcgccg
    47281 gatttcacaa ttcgtccctg atatagcaca tggacgtaat caggcttgat gtagtcgaga
    47341 atgcgttggt agtgcgtaac aatgatgaat gagcgcttgc cgtcacgcag cgagttcacg
    47401 ccatcggcaa ccacttttaa tgcgtcaata tccagcccgg agtccgattc atcaagaatg
    47461 cataactccg gttccagcac cgccatttgc aaaatatcgt tgcgcttttt ctcgccgccg
    47521 gaaaaaccaa cgtttaccga acgggttaat aaatcttccg gcatcttcag gagagcgatt
    47581 ttctcttcca tcaaatcctg aaaatcaaag cggtcgagcg tttcctggcc gcgatagctg
    47641 cgcaccgcat taagtgccgt ttgcaggaaa aactggttac tgacgccggg aatctccacc
    47701 ggatactgga aggccataaa gatgccttcg cctgcacgat cttccggcga cagcgcaagc
    47761 aaatctttgc ctttgaactc aaccgtgccg cccgtcactt cataatcttc tcgcccggca
    47821 agcgttgccg ataaggtact tttgcccgaa ccgtttggcc ccataatggc gtgaacttcg
    47881 ccgggacgaa cgtcgaggct taatccacgc aggatagctt tatcttccac gctgacgtgt
    47941 aaatctttaa tacttaacat gtttattcct tatccgacgc tgtgttcaag actgatggcg
    48001 aggagttttt gtgcttcaac ggcaaattcc agcggcagtt ctgagaacac gtctttgcag
    48061 aaaccgttaa caatcatcga gatggcgtct tcttcgctaa tcccgcgttg caggcagtaa
    48121 aacagttgat cttcaccaat acgtgatgtc gttgcctcgt gctccagttg tgcgctgtta
    48181 ttgcgacatt cgacatacgg gaaggtatgc gccccacaat tagcgccaat cagcattgag
    48241 tcgcactgag tgaaattgcg cgcattggtt gccgtcggca tgattttcac taagccgcga
    48301 taactgttct gactatgtcc ggcagagatc cctttcgaga taatggtcga tttggtgttt
    48361 ttaccgatgt ggatcatctt ggtgccggta tccgcttgct gatgaccgct ggtcagcgcc
    48421 actgagtaaa actcaccaat ggagttatcg ccgcgcaaaa tgcagctggg atatttccac
    48481 gtaatcgctg acccggtttc tgattgcgtc catgacattt tgctgttttc gccttcgcac
    48541 aaagcacgct tggtgacgaa attgagaata ccgccggtgt tgttatcgcc agggaaccag
    48601 ttttgtaccg tggaatattt cacctcggcg tttttatgga tgatgacttc caccaccgcc
    48661 gcgtgtaact gatagctgtc acgcaccgga gcggaacagc cttcaatgta gctgacgtag
    48721 ctgtcttcgt cggccaccag aatggtgcgc tcaaactgcc cggttttttc cgcgttaatg
    48781 cgaaaatagg tggaaagttc catcgggcag cgcacgcctt taggcacata aataaacgta
    48841 ccgtcagagg ctaccgccgc attaagcgcg gcaaagaagt tgtcatttcc cggcaccaca
    48901 gtgccgagat atttacgcac cagttccgga tgatcgtgga tcgcctcacc aaaggaacag
    48961 aaaataattc cctgctccgc cagtttttcg cgataagtag tggcaaccga aacagagtcg
    49021 aaaatggcat ccaccgccac ctctttgcct tcccgcacgg gaacgcccaa ctgctcaaac
    49081 gccgcctcca cctctttact taaaaaggcg ttcgcgccag tttgctgcac cgcgccaggt
    49141 tcagacgcgc aagtgtcgtc acaattaccg cacgatggtg ctgagtagta actgtaatcc
    49201 tgataattca gcttgtcgta gtgcgctttc aaccagtgcg gttcttccat ctccagccat
    49261 gcgcgatagg cgtttagacg aaactccagc atccactccg gctcattacg cttcgccgaa
    49321 attgcgcgca ccacctcttc gtttatcccc tttgccagct catcggtggc taactgggtg
    49381 aagaatcctt ctttataatt cagcgggccg ccggtccagg ttttgacatc gtcagttgct
    49441 tcagtattac gagacatagt accgcctata ccccaaagct ttcgccacag ccacattcat
    49501 tctgggcttt agggttgtga aatttgaata tctgattaag tccttcacga acgaaatcga
    49561 cttccgtgcc atcaataaac ggcatcgctt gcagcgggac aaacagcttc gcgccgtcgt
    49621 gttcaaacag cagatcgtct ttgtccggct cgctaacact gtcgagcaca tagccaaagc
    49681 ccgcgcagcc cgtttgcttc acgcctaagc gcacgccgac catacccggc tgctttgcca
    49741 ccagctcacg gatgtgtatc gccgctgcgg gtgtcagcgt taagccttgc caggcgaaat
    49801 cttgtgggtt aaaggttcct gaatgcatgt ccatcgattt acctcacttc atcgctttca
    49861 gcgtataaca gcatgctagt gataatgatt atcagttcaa cccagcaaac gcaggggctt
    49921 taccgtaaaa catgcttttt gcctgctttt aataagcata gaccctgatg tgtgggttaa
    49981 caggcacgct aagaattagg tatctcattg ttagataatg gttattatct aaggagcatt
    50041 aaggctgtga tgaaaaagaa aaagttgtat tgaaaatgac tatttaagag ataggtaaaa
    50101 aagtgcagcg ttcagaaata agaaaaccct taagtctgtg cgacacaggc ttaagggttt
    50161 ctaccccatc cggcgcttat ctccggcact ctcagtggct tagctcttga aggggcgata
    50221 agaataatct cataaagcta acccgccgtt ttaacacaaa ctgcgattag tattattttt
    50281 gaacaatatc aggcggtaga taagcagtat taagaaggtc atcgaacctg gacggaggtt
    50341 aatccaggtc gatttggcga acttgcggca ttaagtcagg atcaatgcac gcccagacgc
    50401 caggcaaagt agatttcttc ttttaattca gtagaagaga gagtaagcag gtcagcaaat
    50461 tcaagttcta gttgtttcag acgtttgaga tattgggcag gtgaaagatt gctctggtca
    50521 cggcgtaaaa attcaatggc cagctgggtg ggatcaagtt gagtagacat agcatcctcg
    50581 cttttagaca agacgtgcac agtataccac cgtttactgt gcagataatg accaaaagca
    50641 atatgcgtca cacttttctg gtgacaacgt cacaaaatgg cggtcgtcaa tcgtgacgaa
    50701 cagcacaaac gccctttctc atcgaagatt tcaatctgcc agacctggtg acgcgaaccg
    50761 agatgcaacg gtttacatat gccgcgcacc cgcccttctc gtgccgagcg gacgtgatta
    50821 gcattgattt ccagaccaac cactttttgc tcaccttcgg tacataaata accggcaacg
    50881 gaaccgatac tttcggccag taccacggat gctcctccat gcagcaaccc gaaaggctgc
    50941 tttgtccgcg agtctactgg cattgtcgct tcaagggtgt catcaccaat atgttcaaag
    51001 cgaatatcca ggaaccccac catgtttcct tcacccatag cattcagtgc ttccagggtg
    51061 attttccgtt tccatatcat ttaataatct ccagtaaagc ctgcacagga tggcgtaccc
    51121 ccgtgccttc aacccgtttt acctggctac ggcaggaata tccggtcgcc agacagcggt
    51181 tacgcggcag tcgctgcata gcctgatgcc aggataactc atagatcccg agcgaatttt
    51241 catggttttt cgcttcatgt ccgtaagtcc ctgccatgcc gcagcaaccc acgctgacat
    51301 tttccagttt cgcgccaaaa cgggcaaata tcgcggccca ttgtgctggc gcgcccggca
    51361 aagcggtaac ctcggtacag tgaccaaaga aataccatga ttcaccgctg actgtagcca
    51421 ctggatgtga ctcaagtgcg cttgccagcc attcattcgc aagtaagacg ttaaactcgc
    51481 cacgttcctc gcccagcgcc agtttatatt catcgcgata acaaagtacc agcgccggat
    51541 cgacgcccac cattggcatc ccaagcttag ccatacggtt gaggaaatcc gccgtctttt
    51601 tcgccgtctt cgcaaaacga ttaagaaagc ctttgatatg ctgggctttg ccatttggcg
    51661 aaaatggcag taacacaggc tggaaaccta atttttcaac cagacggaca aaatccgcca
    51721 ccacttgcgc atcgtaatag ctggtaaagg ggtcctgcac caccaacacc gtgcgcgctt
    51781 tctgctctgc attgagagct tcaagctgtt ccagcgtcat gtttgccgag cgatgcccca
    51841 ccatttgttg ttgtagcgag gggaccgaca gcagtggcaa atcaaccatg ccgatatgtt
    51901 tttccgagag tttgcgcacc agcggctggt taatgaagaa gttaaaggtt ttcggcgcgc
    51961 gtgccatcag cggtgcgtag ctctcgaccg tagcgacgag gtggtcgcgc agcgggcgta
    52021 aataacgggt gtgatagagc tgcaggaaac gagagcgaaa ctccggcaca tcaattttga
    52081 tggggcactg ggtcgaacac gctttacagg ccagacagcc cgacatcgcc tctttgactt
    52141 cgtgtgagaa gtcatattcg cctttattcg catgccagct attgcgcgtg cgggcaatta
    52201 acgtccgtaa actgacgccg gattcaggca gttcttgttc cagtttgagt ggatcaacgc
    52261 cgcgatccgc caacaaacgc agccattcac gcaccagcgt tgcgcgccct ttcggtgaat
    52321 gaatccggtt ctgggtgatc ttcatcgacg gacacatcgg actacgggca tcaaagttga
    52381 agcataaacc gttgccgtta cactccatcg caccgcgcca ctgctggcgt accgcaatgg
    52441 ggatctgccg atcgaatgta ccgcgcttca ccgcgtccac tttcatcatc ggcgcatcga
    52501 gaccttctgg cgggcaaatc ttccctgggt tgagtcggtt atgcgggtca aatgccgctt
    52561 tcactttgcg cagttctgca aaaagttcct caccgaaaaa cgccgggctg tattcagcgc
    52621 gaaaaccttt gccgtgctcg ccccacaaca aaccaccgta tttcgcagtc agcgccacca
    52681 cgtcatcaga gatttgcttc atcaaaatct cttgttgcgg atcgcacata tccagtgccg
    52741 gacggacgtg caaaacacct gcgtcgacgt gaccgaacat accgtagctt aagccgtggc
    52801 tgtcgagcag cgcgcgaaat tcagcaatat aatcggccag atgttccggc ggtacgcagg
    52861 tatcctcagc aaacggaatt ggcttagcgg cacctttggc attgccaagc agaccaacgg
    52921 cttttttgcg cattgcatag atacgttcaa cccccgccag ctcgcggcac acctgccagc
    52981 caatcacacc tgcctgttgg ctggcaatca gctcatcaag ccgcacacag agtgcattaa
    53041 ctcgctcgtc aatcagcgcc tcatcatcac cagcaaattc cacaatgttc agcccgagca
    53101 tctctttgtc aggcacatcg gtaatcaact cgctgacgga atgccagaca atatcttccc
    53161 gcgccagatt cagcactttt gagtccaccg tctctaccga aagcgcccgc gcctcaacca
    53221 taaacggcgc gttacgcagc gcggagtcaa aagagtcata tttgacgttc accagacggc
    53281 gcactttagg caagggcgta atatccagcc gcgcttcggt aataaaggcc agcgtccctt
    53341 ctgaacccgt cagaatgcgc gtcaggtcga actcggtcat ctcatcgtta aagacatgac
    53401 gcagatcgta accggtaaga aagcggttaa gtttggggaa gttgtcgatg attaactggc
    53461 gttgcagacg gcaacgttga taaaccgtgt tataaattcg cccgattgtg gtattggttt
    53521 tacccagcgt ttccgccagt tcgacgggta aaggttgcgt atcgagaata tcgcccccca
    53581 acaacaccgc gcgtacgcca agtacgtgat ctgacgtttt gccatagacc agcgatccct
    53641 gaccggatgc atcggtattg atcatcccgc cgagcgttgc ccggttgctg gtcgaaagtt
    53701 ctggtgcaaa aaagtagccg aacggtttca ggtactgatt gagttgatct tttatcaccc
    53761 cggcctcaac gcgcacccag ccctcttcag ggttaatttc gatgatgcgg ttcatatggc
    53821 gggacatatc aacaataatc ccctggttga gcgcctgacc gttagtgccg gtgccgccgc
    53881 cgcggggggt aaagatcagc gatgaatagc gttcctgcgc ggcaagacgg gcgatcagcg
    53941 ccacatctgc ggttgaacgc ggaaatacca ccgcatcggg gagaagttgg taaatactgt
    54001 tgtcggtcga cattgtcaga cgatcggcat aacttgtcgc cgtatcgccg gtaaaacctt
    54061 gttgctccag ctcttgcaaa aaattaagca ccagttgaac gacgccgggt gcctgggaaa
    54121 tctgtggaat cattatattg accctttcct gcggtctgtg atgtaggtcg atacactatt
    54181 ctttcaggcg gctgcaatag cgcactgaaa ggtgatgttt atttactcta tggatttcga
    54241 gttgcaggaa ggcggcaagc gagtgaactc caggagctta caatagtaag tgactggggt
    54301 gaacgaacgt agccgcagcg catgcaactt gaaatacgac gagtaaatcg tttgcgtgtt
    54361 gcctgagttg ttgtaccaca tttttttcta acacgcccat cagaattaag ggcagaatcg
    54421 gcctgttaaa aaccgctgaa attgctcatc attatgcagg tgagtttcgc gtgttcacgt
    54481 cgcgtcgacg atttgacgca caaaaaaggt gaaaagtagt tatggtaaat gttcgtcagc
    54541 ccagggatgt cgcacaaatt ctgctttcgg tgctgttttt agccatcatg attgtggcat
    54601 gtctgtggat tgttcaaccc tttattctcg gctttgcatg ggccggtacg gtggttatcg
    54661 ccacctggcc ggtattgtta cgtttgcaaa agatcatgtt tggccgccgc tctctcgccg
    54721 ttctggtgat gacgctgtta ttagtgatgg tgtttatcat ccctattgct ttgctggtta
    54781 acagtatcgt cgacggcagc ggcccgctaa ttaaagccat ttccagcggc gacatgacgt
    54841 tacccgatct ggcgtggctt aataccattc cggtgattgg cgcgaagctg tatgcaggct
    54901 ggcacaactt gctggatatg gggggtacgg cgatcatggc gaaagtccgc ccttatattg
    54961 gcaccaccac cacctggttc gttgggcagg cggcgcatat cgggcgcttt atggtgcatt
    55021 gtgcgctgat gcttctcttc agtgccctgc tgtactggcg cggtgaacag gtggcacaag
    55081 gcattcgcca ttttgcaacc cgtctggcag gcgttcgcgg agatgccgcc gtgctgctgg
    55141 cggcacaggc tatccgcgcg gtggcgctgg gtgtggtggt gacggcgtta gtacaggcag
    55201 tgcttggcag tatcggcctc gccgtatccg gcgtacctta tgcaacgttg ctaacggtgt
    55261 taatgatcct ctcctgcctt gtccagcttg gcccgttgcc ggtactgatt ccggcgatta
    55321 tctggctcta ctggaccggc gataccacct ggggaacggt attgttagtg tggagcggtg
    55381 tggttggcac gctggataac gtcatccgcc caatgttaat tcgcatgggt gccgatttac
    55441 cgctgatcct gatcctctct ggcgttattg gtggtttgat tgctttcggg atgatcggtc
    55501 tgtttattgg tccggttctg ttagccgttt cctggcgtct gtttgccgcg tgggtggaag
    55561 aagtcccgcc gccgacggac caaccggaag aaattctcga agaacttggc gaaatcgaga
    55621 aaccgaataa gtaatttctc atcaggcggc tctgccgcct gattgttaat caccgctaat
    55681 tgatatttgc ttgctcttcc ccatattttc ctgcttaccg ccattcagct
//
LOCUS       DAESHI010000016        54950 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.016, whole genome shotgun sequence.
ACCESSION   DAESHI010000016 DAESHI010000000
VERSION     DAESHI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54950
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.016"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="KW011_000986"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="KW011_000986"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBH7659400.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="KW011_000987"
     CDS             complement(1672..2730)
                     /locus_tag="KW011_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBH7659401.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="KW011_000988"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="KW011_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="KW011_000989"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="KW011_000989"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HBH7659402.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="KW011_000990"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="KW011_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HBH7659403.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="KW011_000991"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="KW011_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HBH7659404.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="KW011_000992"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="KW011_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HBH7659405.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="KW011_000993"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="KW011_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HBH7659406.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKNN"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="KW011_000994"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="KW011_000994"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HBH7659407.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="KW011_000995"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="KW011_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HBH7659408.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="KW011_000996"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="KW011_000996"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HBH7659409.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="KW011_000997"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="KW011_000997"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HBH7659410.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="KW011_000998"
     CDS             complement(17699..18322)
                     /locus_tag="KW011_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HBH7659411.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /gene="ypdI"
                     /locus_tag="KW011_000999"
     CDS             18619..18894
                     /gene="ypdI"
                     /locus_tag="KW011_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HBH7659412.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="KW011_001000"
     CDS             complement(18971..19213)
                     /locus_tag="KW011_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HBH7659413.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="KW011_001001"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="KW011_001001"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HBH7659414.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20842..20913
                     /gene="ypdK"
                     /locus_tag="KW011_001002"
     CDS             20842..20913
                     /gene="ypdK"
                     /locus_tag="KW011_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HBH7659415.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20978..22216)
                     /gene="alaC"
                     /locus_tag="KW011_001003"
     CDS             complement(20978..22216)
                     /gene="alaC"
                     /locus_tag="KW011_001003"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HBH7659416.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22593..24290
                     /gene="ypdA"
                     /locus_tag="KW011_001004"
     CDS             22593..24290
                     /gene="ypdA"
                     /locus_tag="KW011_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HBH7659417.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            24305..25039
                     /gene="ypdB"
                     /locus_tag="KW011_001005"
     CDS             24305..25039
                     /gene="ypdB"
                     /locus_tag="KW011_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HBH7659418.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            25052..25909
                     /locus_tag="KW011_001006"
     CDS             25052..25909
                     /locus_tag="KW011_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH7659419.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(25912..28407)
                     /gene="ptsP"
                     /locus_tag="KW011_001007"
                     /pseudo
     CDS             complement(25912..28407)
                     /gene="ptsP"
                     /locus_tag="KW011_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(28432..29469)
                     /gene="ypdE"
                     /locus_tag="KW011_001008"
     CDS             complement(28432..29469)
                     /gene="ypdE"
                     /locus_tag="KW011_001008"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBH7659420.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29469..30554)
                     /gene="ypdF"
                     /locus_tag="KW011_001009"
                     /pseudo
     CDS             complement(29469..30554)
                     /gene="ypdF"
                     /locus_tag="KW011_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30569..31816)
                     /locus_tag="KW011_001010"
     CDS             complement(30569..31816)
                     /locus_tag="KW011_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBH7659421.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMLGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31838..32164)
                     /locus_tag="KW011_001011"
     CDS             complement(31838..32164)
                     /locus_tag="KW011_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HBH7659422.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32384..33349)
                     /gene="glk"
                     /locus_tag="KW011_001012"
     CDS             complement(32384..33349)
                     /gene="glk"
                     /locus_tag="KW011_001012"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HBH7659423.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            33553..34809
                     /locus_tag="KW011_001013"
     CDS             33553..34809
                     /locus_tag="KW011_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HBH7659424.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34924..35250
                     /locus_tag="KW011_001014"
     CDS             34924..35250
                     /locus_tag="KW011_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBH7659425.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35391..36629)
                     /locus_tag="KW011_001015"
     CDS             complement(35391..36629)
                     /locus_tag="KW011_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBH7659426.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36965..38167
                     /gene="nupC"
                     /locus_tag="KW011_001016"
     CDS             36965..38167
                     /gene="nupC"
                     /locus_tag="KW011_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HBH7659427.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38217..40405)
                     /locus_tag="KW011_001017"
                     /pseudo
     CDS             complement(38217..40405)
                     /locus_tag="KW011_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40614..40689)
                     /locus_tag="KW011_001018"
     tRNA            complement(40614..40689)
                     /locus_tag="KW011_001018"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40654..40656),aa:Ala,seq:ggc)
     gene            complement(40729..40804)
                     /locus_tag="KW011_001019"
     tRNA            complement(40729..40804)
                     /locus_tag="KW011_001019"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40769..40771),aa:Ala,seq:ggc)
     gene            41025..41384
                     /locus_tag="KW011_001020"
     CDS             41025..41384
                     /locus_tag="KW011_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBH7659428.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41386..41778
                     /locus_tag="KW011_001021"
     CDS             41386..41778
                     /locus_tag="KW011_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBH7659429.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41830..43245)
                     /gene="gltX"
                     /locus_tag="KW011_001022"
     CDS             complement(41830..43245)
                     /gene="gltX"
                     /locus_tag="KW011_001022"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBH7659430.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43504..43579
                     /locus_tag="KW011_001023"
     tRNA            43504..43579
                     /locus_tag="KW011_001023"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43537..43539,aa:Val,seq:tac)
     gene            43624..43699
                     /locus_tag="KW011_001024"
     tRNA            43624..43699
                     /locus_tag="KW011_001024"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43657..43659,aa:Val,seq:tac)
     gene            43746..43821
                     /locus_tag="KW011_001025"
     tRNA            43746..43821
                     /locus_tag="KW011_001025"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43779..43781,aa:Val,seq:tac)
     gene            43826..43901
                     /locus_tag="KW011_001026"
     tRNA            43826..43901
                     /locus_tag="KW011_001026"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43859..43861,aa:Lys,seq:ttt)
     gene            43932..44057
                     /locus_tag="KW011_001027"
     ncRNA           43932..44057
                     /ncRNA_class="other"
                     /locus_tag="KW011_001027"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            44177..44518
                     /locus_tag="KW011_001028"
     CDS             44177..44518
                     /locus_tag="KW011_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HBH7659431.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(44509..45435)
                     /locus_tag="KW011_001029"
     CDS             complement(44509..45435)
                     /locus_tag="KW011_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH7659432.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGWEGAPLYQI"
     gene            45525..46523
                     /locus_tag="KW011_001030"
     CDS             45525..46523
                     /locus_tag="KW011_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HBH7659433.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46520..46738)
                     /locus_tag="KW011_001031"
     CDS             complement(46520..46738)
                     /locus_tag="KW011_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HBH7659434.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46740..48755)
                     /gene="ligA"
                     /locus_tag="KW011_001032"
     CDS             complement(46740..48755)
                     /gene="ligA"
                     /locus_tag="KW011_001032"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBH7659435.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48826..49812)
                     /gene="zipA"
                     /locus_tag="KW011_001033"
     CDS             complement(48826..49812)
                     /gene="zipA"
                     /locus_tag="KW011_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HBH7659436.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            50042..50803
                     /gene="cysZ"
                     /locus_tag="KW011_001034"
     CDS             50042..50803
                     /gene="cysZ"
                     /locus_tag="KW011_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HBH7659437.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50988..51959
                     /gene="cysK"
                     /locus_tag="KW011_001035"
     CDS             50988..51959
                     /gene="cysK"
                     /locus_tag="KW011_001035"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBH7659438.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            52343..52600
                     /gene="ptsH"
                     /locus_tag="KW011_001036"
     CDS             52343..52600
                     /gene="ptsH"
                     /locus_tag="KW011_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HBH7659439.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52645..54372
                     /gene="ptsI"
                     /locus_tag="KW011_001037"
     CDS             52645..54372
                     /gene="ptsI"
                     /locus_tag="KW011_001037"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HBH7659440.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            54413..54922
                     /gene="crr"
                     /locus_tag="KW011_001038"
     CDS             54413..54922
                     /gene="crr"
                     /locus_tag="KW011_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH7659441.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT        14332 a        13802 c        12950 g        13866 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgtcata
      121 gttaacgtta acatttgtga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaacaatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgccacctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagcg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat tgtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg gaccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tgttttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcggac aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaggagaac ctgataagcg acaaaggctg cacgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgggcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aggcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataacgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccgctgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctag
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccagc tatgcaaaaa cggctggaaa aagaatatca
     3421 gcaaccgatc agcgggcaac tgttactgaa aaaagatagc catttgccca tttccggctc
     3481 cataaaagca cgcggcggga tttatgaagt cctggcacac gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc ggcactggcg ggtatggccg gacctcagcg
     4321 cgtatgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aactgcgtaa
     4381 taccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccataata
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctgaaaa aactgcggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaacta aatgtcacta acaaccgcat
     5101 gtctattttg ttgccataac gtcctatcaa aggtgaaatt aatagtggca tgatgccgat
     5161 gggcgcataa gcaagtccgg cccatatcgc attatacccc atcgtttcct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg catgtgatac tcacaatacc
     5281 aatggtgaag ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ctcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcctggtag attcattttg accggtgaag tctcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc acaaatataa ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 cggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaaccc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataggctac acattaatga
     5821 agacagcgaa aaaaaggtta ctgaaagtaa aaataatctt aattcgccta ttctttgtgc
     5881 caacctgcca gtaacaggga tcgcaatggc atttgctaca ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaatgt cgctaatgac aatgcaatag tgacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagccggcat ggaggtcacg gttgaagcca gctcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccgggac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcgcc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaggatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc tggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacttat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgca
     8281 caacgtaaca aaatcggcta accacatgaa gtttttaccc tatatttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcatac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acataagtcc caaacggcta cgttgttgca
     8521 taccgattcg cagcaacgag ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 aacgttaaat atcaaattaa cactccggga atacgccgat catcaaaaag caatggacgc
     8641 gctggaagaa ggtgaagtcg atatagtgtt atcacattta gtagcttcgc cgcctcttaa
     8701 tgatgacatt gctgcaacca aaccactgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgattca atgcgaccgc ttacctcatc aaaaccagta aatattgctc gagtagcaaa
     8821 ttatccccct gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agccggacag aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcaccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcgttct
     9061 taatgaagta ctcaatcgat ttgttgatgc tttgacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa caatggatta agcagcatcc cgatttaaag gtgctggaaa atccttactc
     9241 ccctccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggatattct
     9301 taatattatt actttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacagctga atcccggcgg atgggatata ttacccggtg ctatttatag
     9421 tgaagataga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagcttc attcgcaatt aaaagagatg tatccggagg tagagtggat
     9601 aaaagtcgat aacgccagcg ctgcatttca caaggtcaag gaaggcgaac ttgatgctct
     9661 ggtcgcgaca cagttaaatt cacgttacat gatcgaccat tactatccta atgaacttta
     9721 tcattttctt attcccggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca atccccccaa gcgaagttct
     9841 gcgcctgacc gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctcta
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgac tcccccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 ttttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tctgccagcc agcgatcatg ctgtttatat ttgtggttgg caagatatta cagagacgcg
    10381 tgatttaatt catgcactcg aagtagagag aaataaagcg atcaatgcaa ctgtcgcaaa
    10441 aagtcagttt ctggcaacaa tgagtcacga aataagaaca ccaataagct ccattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac tccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaatttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagca atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg gttcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactcttt aaacgctaca gccaaacaag
    10981 tgcaggtcgc cagcaaacag gatctggctt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttgt cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaccc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaacgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca agcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgtttcagat gacagtaagc ctgaaattct
    11821 tcagttgctg aactctgtaa aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aataactaaa tagcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaagcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctgggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattacaaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggtcgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagcggc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgcagc agggtcgatg accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccga atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attatttcat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atatttattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca ctggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ttcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acaaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgcgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cctaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcctatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatggttt ttatggcttt tttcttaagg ttttttcctt cgctgaatat
    20581 ctaattatta ccgtcagaaa ttaaactgtc gccgatatga gacacttgta agttactaaa
    20641 agtaaataag aaaatgcctc ttgtctcgtt tcagttttag gcgtacatta acgccgtctt
    20701 gagcagttta gctcagaatt ctggggtgat gcccacggta aaatacaggg cttttaaggt
    20761 cgctttgccc ggtgtcaact cactgtatca ggtgtaatga agtcattcag gcgtaacagt
    20821 aattacgcgg agagatgtaa agtgaaatat ttctttatgg gcatttcttt tatggtcatc
    20881 gtttgggccg gtacttttgc cctgatgatc taaaagcaaa ctgtgcaatg aaaaacagga
    20941 gccatcaggc tcctgtttgc atttctgacg acggtgttta ttccgcgttt tcgtgaatat
    21001 gtttgctgct ggcgggtagt aaaccgtcgg cacggaacat cgctttaatc cctctgatgg
    21061 cctgacgaat acggtcgcgg ttttcaatca gtgcaaagcg aacatgcgta tcaccatagt
    21121 cgccaaagcc aatccccggc gagacacaaa ccttcgcttc gttaaggagc ttcttggcaa
    21181 attccagcga tcccatggcc gcatatggtt ccgggatttt cgcccagaca tacatcgaag
    21241 ccttcggcat ttcgaccatc cagcccgctt catgcagccc tttaaccaat acatcacggc
    21301 ggcgtttgta ctgttcggta atgtcgcgca cgcactgttg atcgccctcg agtgccgcaa
    21361 tcgccgccac ctgcaacggc gtaaaagtgc cgtaatcgtg atagcttttg attcgcgcca
    21421 gggcactgac cagcgttttg ttgccgacca taaagccgat acgccagccc gccatgttgt
    21481 agcttttcga cagcgtaaag aactcgaccg ccacatcgcg tgcacccggc acctgcataa
    21541 ttgacggcgc tttccagcca tcgtagacga tatcggcata ggccaggtca tggaccacca
    21601 gcacatcgta gcgtttcgcc agcgccacaa ccttttcgaa gaactccagt tccacgcatt
    21661 gcgcggttgg gttcgacggg aagccgagga tcatcatctt cggtttcgga taactttcac
    21721 gaatggcgcg ctccagttcg ttgaagaaat cgacaccttc caccagcggc actgagcgta
    21781 cctgcgcccc ggcaatcacc gcgccataaa tatggatcgg gtaacttgga ttcggcacca
    21841 gcaccgtgtc accatgatcc agcgtcgcca gcatcagatg cgccaggccc tctttcgaac
    21901 caatagtgac gatggcttct gattccgggt cgatttcaac gtcgtagcga tcctgatacc
    21961 agcgggaaat ggcgcgacgt aaccgcggaa tgccgcgtga agtggagtaa ccatgcgtgt
    22021 ccgggcgctg ggccacagta cataattttt cgacgatatg cggcggagtc gcaccgtccg
    22081 ggttacccat gctgaaatcg ataatatctt cgccgcgccg acgcgcagcc attttcagtt
    22141 cagcggtgat gttaaaaacg tagggcggga gacgatcaat gcgcgtaaag cgacgttcag
    22201 ggcgagtgtc agccataatt tcctcagatt aacgttagcg cccggaccgt ccgagcgacg
    22261 ctgccacaat ggtggctgtt ttgaaaatag cctgattaat ttctgtctgt ccagcgtttt
    22321 agaacatttt tatcaatggc tttaaatgag gaactccact tcaaaaacag gaaatttttc
    22381 tacgctaaca agaaaattac caccaatttt attaacatta taatttcaat aaatttacca
    22441 gccagaagtg cattatcaag agatacattg ctaatacacc atttatcttt caatcccctt
    22501 ttaaatttat ggtttttcct caaaaacggc ctgctggtca tcagccttca ggttacctat
    22561 catagaggtt taatccttat tcagagtcac ccgtgcacga aatattcaac atgctgctgg
    22621 cggtcttcga tcgggcagcg ttaatgctta tctgcctgtt ctttctcatc cgtatccgcc
    22681 tgtttcgcga actgttgcac aagtcggcgc actccccaaa agaattgctc gccgttaccg
    22741 ccattttctc gctgttcgcc ctgttcagca cctggtccgg cgttcccgta gaaggctcgc
    22801 tggtgaacgt acgtattatc gcggtgatgt ccgacgggat tctgtttggc ccgtgggtag
    22861 gcatcattac cggcgtgatt gcgggtattc accggtattt aattgatatc ggcggcgtga
    22921 cggcgatccc ctgctttatc accagcattc tggcgggttg tatatcaggc tggataaacc
    22981 tgaaaatccc caaagcacag cgctggcgcg tcggtattct cggcggcatg ttgtgtgaga
    23041 cgttgaccat gattctggta attgtctggg caccaactac cgcgttgggg atcgatatcg
    23101 tctctaaaat cggcattcca atgatcctcg gtagcgtctg tatcggcttt attgtgcttc
    23161 tggtgcaaag cgttgagggc gaaaaagagg ccagcgccgc gcggcaggcc aagctggcgc
    23221 tggatatcgc caacaaaacg ctaccgctgt ttcgccatgt caatagcgag tcattacgca
    23281 aggtctgcga aattatccgc gatgacattc acgccgatgc ggtggcgatt actaataccg
    23341 atcatgtgct ggcctatgtt ggcgtgggtg aacataacta tcagaatggc gatgacttca
    23401 ttagcccgac tacccgtcag gcgatgaatt acggaaaaat catcattaaa aacaatgatg
    23461 aagcccaccg cacaccagag attcattcca tgctggtgat cccattgtgg gagaaagggg
    23521 tcgtgaccgg aacgctgaaa atttactact gccacgcgca tcagatcacc tcgtcattac
    23581 aggaaatggc ggtcggtctg tcgcaaatca tctccacgca actggaggtt tcacgcgccg
    23641 agcagctacg tgaaatggca aataaggcag agcttcgcgc cctgcaaagc aaaattaatc
    23701 cccattttct gtttaacgct ctgaacgcta tttcatcgtc aatccgtctg aatccggata
    23761 ccgctcgcca gttgatcttt aatctgtcgc gttatctgcg ctataacatt gaattaaaag
    23821 acgatgagca aatcgatatc aaaaaagagc tgtatcaaat taaagactat attgccattg
    23881 agcaggcccg ctttggtgac aagctgacgg ttatctatga tattgatgaa gaggtgaatt
    23941 gctgcattcc cagcctgctg atccagccgt tggtggagaa cgccattgtc cacggtattc
    24001 agccttgcaa aggtaaaggc gttgtcacca tcagcgttgc agagtgcgga aatcgggtac
    24061 gcattgcggt gcgagatacc gggcacggca tcgatccaaa ggtgattgag cgggtcgaag
    24121 cgaatgaaat gccgggcaat aaaattggcc tgctgaatgt ccatcatcgc gtgaagttat
    24181 tgtatggcga ggggctgcat atccgccgcc tggagccggg gacggaaatt gcgttttaca
    24241 ttcctaacca acgcacccca gtcgcctcac aggctacgtt attgctttga gccggagtga
    24301 tattgtgaaa gtcatcattg ttgaagacga attcctggca caacaggaac tgagctggct
    24361 aattaaagag cacagccaga tggagattgt cggcaccttt gacgacggtc tggacgtgtt
    24421 gaagtttttg cagcataacc gcgtcgacgc catttttctg gatatcaata ttccgtcgct
    24481 ggatggcgtg ttgctggcgc aaaacatcag ccagttcgcc cataaaccgt ttattgtgtt
    24541 catcaccgcg tggaaagaac atgcggtaga agcgtttgaa ctggaggcgt ttgactacat
    24601 tctcaaaccg tatcaggagt cacgtattac cgggatgctg caaaaactgg aagcggcctg
    24661 gcaacaacag cagaccagca gtacgcctgc cgcgacggta acgcgtgaga atgacaccat
    24721 taatctggtg aaagatgagc gaataatcgt cacgccaatt aacgatatct attacgccga
    24781 agcgcacgag aaaatgacct ttgtctatac gcggcgtgaa tcctacgtaa tgccgatgaa
    24841 cattaccgaa ttttgcagca aactgccgcc gtcgcatttt ttccgctgcc atcgctcatt
    24901 ttgtgtcaat ctgaacaaaa tacgcgaaat cgaaccgtgg tttaataaca cctacattct
    24961 gcgactgaaa gatctggatt ttgaagtgcc ggtcagccgc agcaaagtga aagaatttcg
    25021 ccagttaatg catctttaat cgggaacttt catgaaagca ccaggcttgc ccgccgatca
    25081 acaatttttc gccgatctgt tcagcggcct ggtgcttaac ccgcaactac tggggcgcgt
    25141 ctggtttgcc agccagcctg cctcgttacc ggtgggcagt ttatgtattg attttccccg
    25201 tctggatatc gtgctgcgcg gcgaatacgg caacctgctg gaagcaaagc agcagcgaat
    25261 ggtggaagga gaaatgctgt ttattccggc acgcgcggct aatttaccga tcaacaacaa
    25321 accggtgatg ctgttaagcc tggtgttcgc accgacctgg cttgggttat cgttttacga
    25381 tagccgcacc acgtcgttgt tgcatcctgc tcgccagatc cagcttccca gcctgcaacg
    25441 cggtgaaggt gaagcgatgc ttaccgccct cacccatctt agccgttcgc cgctggagca
    25501 gaatatcatt cagccactgg tgttaagttt gctgcatctt tgccgcaacg tggtgaatat
    25561 gccgccgggt aattcgcaac cgcgcggcga ttttctctat cacagcattt gtaactgggt
    25621 tcaggataat tatgcccagc cgctcacccg cgagagcgtg gcgcagtttt ttaatatcac
    25681 gcccaatcat ctgtcaaaac tgtttgctca gcatggaacg atgcgtttta tcgagtatgt
    25741 gcgttgggtg cgaatggcga aggcgaggat gattttgcag aaatatcatc tgtcaattca
    25801 tgaagtggca cagcgttgcg gttttccgga tagcgactat ttttgtcgcg ttttccggcg
    25861 tcagtttggt ctgacgccgg gagagtacag cgcccgtttt cagggctaac gtcagaaggt
    25921 taattctgtt tccagcagcg tcaggatact ttgtgcatct tgcgcggcaa acaacgactg
    25981 gcggaagttt ttattcacca gtttacgcgc cagctgcgag aagactttca catgattcat
    26041 cccttcgtta gcacccagcg tcagcatgat caccagttca acttcgccca tttctgactg
    26101 ccagtcaacc ggtttcgcca gccgggcaat gctgatactg gaatgacgga tccactgaga
    26161 tttggtgtgc gggatcgcta cgccaaaacc aacgccggtg gtaacaatct cttcccgctg
    26221 ccagacatct tcttccagct caaacggatg ttcagtgcgc ccgttaacgc cgaggttgcc
    26281 gcacaggaac tggatcgcct gctctttatt gctaaaatcc tgatcaacaa agatattctc
    26341 cagcgccagc agtgggcgaa cgtcttcttc cggcgtaaag gcggtgagta acgcttcaat
    26401 ttcctgcgca ctgcggcatt cacatgcctg acgcgccagt tcccgacacg cctcgctatc
    26461 cagttgacga agctggcttt tcaccgccgg aatacgcggg ctactcatac tcagctcatc
    26521 caggcccagc ccaagcagta gcggcagata acggctttca ccgcccagtt caccgcaaat
    26581 gcctacccat ttgccccgct gatgcgcagt ggtaactatt tgctgcaaca tgcgcaggaa
    26641 cgatggcgta atcgggttat atagcggcga tacgcgcggg ttattacgat cgaccgcata
    26701 cagatactgg gtcatatcgt tggagccgat actgaagaaa tcgacctcat cggagaagtg
    26761 gtcgatgatg tagcacaccg acggcacttc caccatgatc ccaagcgtaa tcgtctctgc
    26821 atgacgcagg ccatcgcgct taagctcaac gatcgctttt tggatctcgc ctttcaccca
    26881 taagatctga tcgaggctgt gaaccatcgg gatcatcaac tgggcgttgc cgaaactggc
    26941 ggcgcgcaaa atggcccgca gttgagtgcg gaacaggcca gcaaattccg ggtaaatacg
    27001 taccgcgcga tagccgagga acgggttctc ttcctgggga atattcagat aaggaatgct
    27061 tttatcgccg ccgatgtcca tcgtgcggaa gataatcggc ttgtcgcccg ccgccagtag
    27121 cacctgctgg taggcttcaa attgctcctg ctcgtccggc gcgctgtcgc gatccatata
    27181 gagcatttcg gtacggaaca gcccgacacc ttccgcgccg ttggcaaacg cgcctggcgc
    27241 ttccagagcg gtgccgatat tcgccgcaat atcaatacgc ttgttatcac gggaataggc
    27301 cagctgcgcg gcagcctgcg cctgttgttt ttggcgttta tccgccagcg tctgcgcgac
    27361 ctgataataa ccgctcaccg cgtcattcgg gttaatcgcc agcacgccgc actgggcgtc
    27421 aagcactgca ggttgcccgg cataacgggc aatcgcatcc agcggcaagc cactcagtac
    27481 cgggatcgcc gaggcacggg ccagaatcag tgtatgcgag gtgcgcccgg ttttctccag
    27541 aatcatgccc gcaagatttt tcaaatcaag gctcaaaaac tggcttgggg ttaaatcttc
    27601 agccaccaga atggtcggtt tttccagcac cagattgttg cgcggcttca gttccggcta
    27661 ggtgatatgc agcaactgtt cgctgatatc gcgaatgtcg ctgacccgct cgcgcagata
    27721 atcgctggca gaagcagata gtttggcgca aacctgctcc atattgctga tgatcgccgc
    27781 ccccagtccc tgatgctgtt ctgtcatcag gcgacggata ttgcctgcaa attcatcatc
    27841 ctgaatcagc gacaaatgag cgctgaggat agttttgctt tcgccgtcac gctcacgcaa
    27901 ttgctggttc agttgctcgg caagcgttgc caggctgtgc tccagccggg tggaatcttg
    27961 cgcactggcg gggattgccc gataactgtc gaggctgtcg ctttgtaaca gggtcagcgt
    28021 acccacgccg acgccgcttg ccagcacatt gccgtacagt aaatccgggt taaggcggct
    28081 taatgaacgc ggcagcggat gcgccgtcag ttctgcctgc gtcggctgaa cgctgtcgct
    28141 gtcgataaag cgcaccagga tgtactcttc cagcacgcgc cgcgcctgct cttcatcgct
    28201 gccgctaatg ttcaggctgc aactgtcatt aaataaggtg ccagttccaa tcaacgccag
    28261 cgagctttta gcatcggcct ttgcgttctg gcgatggttg ataaaggtga tttcgctttg
    28321 ccactggctg cactgttctt taagttccca cgccggacga gcatgtagac cgttaggcaa
    28381 aggacagaga aattgaatcg ttaacataac cgctccgggt taatcaggag atcatctgaa
    28441 atccgtcagt tgaacaaccg tctcacgcgt aagacgttga ataagggcag ataaaagttg
    28501 ctccatctgc aaaatgtcgc ggcaatcggc aatcgatgcc gcgcaatgtc cgtggcgggt
    28561 ggcaggcccc attaccactg tgggtacgcc agtatcggtt aagtgcaccg ctccaccgtc
    28621 cgtgccgccg ttactgaaca tatccgcctg caacggaacg ccaatttctg ccgacacggt
    28681 ttcaatccac gcggtaagtt ttggcggcgc aatcagcgat ttatcgctta acaccagcat
    28741 cggcccgtta ccaatctgac gatggttagc cgcgccataa tcaaagtttt tcgcccagca
    28801 ggcggtatca agcacaatgg cgacgtccgg cgacaccgcg cgggtagcag tttgcccgcc
    28861 gcgtaatccc acctcttcgc tggaacttgc caccagccac acttccgcag gcagttcggc
    28921 gtcgtgcagc tcacgtagta acgtcaccag cagatagcaa ccgaggcggt catcaaaggc
    28981 tttccccatc acccgctggt gagggagaac ctgaaaagtt gtatcaaagg tgacgcgatc
    29041 gcctggacga attcccgcct gcatcacttc gtcatacgag cgcgcaccaa tgtccacgcg
    29101 catggcgctg acgtcattcc cctgccggtc gccgtcaagc aggcctggaa ttttgcactc
    29161 ttcacgggtg gtgatgcgca ccggctgcag ctggcgggca gccatgcgca cgttgccaac
    29221 cggcagcaca tcaattgccc cttcgcggga gatgctgcgc accataaatc ccacttcgtc
    29281 catatgcgca cagatcatca ccttcggacc tgtcgattca ttgaggcgga tcagcaccga
    29341 tcccagacca tcaaatcgta cttctttttg caggcgatcc gcttcttcca gtaggatctg
    29401 ccgcacttcc tgttccgagg aggcgatcgc atctgcctcg ctcaacgctt ttaatagcga
    29461 taaatccatt atgcctctcc cgtgagcaac actgttttcg gcatggcgta gagcacttct
    29521 gcgccttgcg gggtaaccag cacaacatct tcgatgcgca cgcccccttg ccctggcaaa
    29581 taaatccccg gctccacggt cagtaacatg cctggctgta gcgtcgtggt atcccgcggt
    29641 gaaaagcgtg gatcttcgtg tacttcaata ccgatagcat gaccggtgtt gtgaccaaaa
    29701 tagtcgccaa atcctgcctc ggtaatcacc cggcgcgcgg cgtcgtcaac ctgttagcag
    29761 cgtacgccag ggcgaatcgc ggagattgcc gccagctgtg cttgcaggac aatctgatag
    29821 acgttaaaca gcgggtgaga ttcggcaatt accccttcgc cattcaccag caaggtgcgc
    29881 gtcatatcag agcagtaccc ctgatagagc gcaccaaaat caagagtgac aaactcgcct
    29941 gctgcaacaa tcttgtcgct ggctttgccg tgcggcagcg ccccacgcca gccgctggcg
    30001 acaatggtgt caaaagaggc tttttctgcg ccctgctggc gcataaacca ctccagttca
    30061 gcggctatct cgcgctcgct catccccgcc tgaataaagc ggcgaatatg ctccgctccg
    30121 cgatcggcaa tcccacaggc gaggcggatt atctccacct cctctggcgt tttgatttgc
    30181 cgcagcacat ccggcgtggc gctaaccagt ttcgcattga gttcagactt ccagcggtgc
    30241 gcggtttccc aactcacctg ctggccttca aaaccgagcg tttgcaactg ctcatcggca
    30301 atgatttgat tgacgatagt ggtaagcgtg ttcgtcgcgt caagcaaatg cagctggtag
    30361 ccttgcgcac gggcttccac ctcaacgaaa tagcgcgaat ctaccagaat gtgcgcactt
    30421 tcacggctaa taaccacata gcctgatccg gtggagatcc ccagatgcgg ctgtttgttc
    30481 tgccgtgagg agagaagcac tgcatccagt tgttgcgcct taagccagtc gcgcagcgaa
    30541 gcgagtaatg tcatctggat tttccttctt acaggctatc gattaacaat ttacctttac
    30601 ggaacatcat cagacgcagg aacaccacca tcagcgcagt tatgactgcc cccagcgcga
    30661 tccccgccat atagacgccc aggttagtga ccagcggcca tgcccaaata gcggattccg
    30721 ggaaccattg cactgcgccc agccagacag cggcggttga gccgacaatc gcgccgacca
    30781 tatacgacgg aatggcggtg atggggcttt ccagcgcaaa cggaatcgca ccctcactaa
    30841 tccccataaa ggcaaggaac atcgcggtct taccctgcgg ataaagttga gcgttgaaca
    30901 ggcgtttgcc ggttaaacga cggtcgataa tggtcgccag acccagacct atcggcggaa
    30961 taacgatagc gatagaacgt gcagtgaccg gtaatacgtg gtcagtggta aagctgaagg
    31021 caacaaaacc tgcggctttg ttgatcgggc caccaaggtc gattgcggtc gccgcggcga
    31081 tccccattgc gtacatcagc gcgcccttct cacctgcggc agtcagcacg gtacggatac
    31141 cgccgttgat ccagccgcca aagggggtaa tgacgtagta catcgccagc atgacaaaaa
    31201 tggcggaaag aatcggtagc aaaaatgtgg ttttgaacgc taacaggaaa tccggtaact
    31261 ggattttctg gttcatccac ttcacaaggt aacccgcgac gatagaaata atcagcgcac
    31321 caatgaaggt ggacggcacc ggcgaagagg tcgcccattg catagtgctg ggatcgaagt
    31381 tcagcagttg cgtcggctgg gtagacatca agccgccgat aaaaccagcc gggaacgcca
    31441 gtttgccacc gatagagttc gccacaaaag cagcaaacat cggaatggca aaaccaaaca
    31501 acacgccgcc gaaggactgc gacagccagg caaatttcag taaggagagg tcgaaaccgg
    31561 agaatttccc gctattaaga gcatccatga tgccgatctc agcaggaatt ttaagccagc
    31621 tataagcaat cagctggctg aaagcgagga tcacaccgcc cataattaag gtcggcacca
    31681 tgcgtgaaat ccctaacatc acatgctgcg ggagttcacc ccagaagctg cttttagagg
    31741 cctgcggatt cttaactgcc gctgccgcac cggatgcgcc aggcacaacg gttgcactgc
    31801 gttttttaat ggccataaca attatccctg tcgaatatta ctgttgttca gaggcaatca
    31861 tctcttcgat ttctttgatg atgcccgcag cgtttttaat tgcgtcctgc aaagtgattt
    31921 cataaacgtc gcgtgattcg aaacgctcat tatcttccgg ggtaactgcc acggagtgga
    31981 tgatgatggt tgcttcggcg atatcctgcg ccgtcaggcg attctggata ccgtccgcgc
    32041 cctgggtttc aattttcact tcataacctg cttctaccgc cgcttcttcc agcgcctgag
    32101 cggccataaa ggtgtgagcc aggcccattg ggcaggcaca taaggcaatt agtttcttac
    32161 tcatcgttaa ttcctcgttc agtgatagtt gacgagtgaa attatgaacg tgcaaaacaa
    32221 atcgccgtta ccagacaaat aatgcattta ctggagaatt aatcccgctg tacggaagtg
    32281 tgacgcaggt cggccttgtg agggggacac aaagaaaaat tgaattaact gtatgattta
    32341 aaagattatc gggagagtta cctcccgata taacagaaag gatttacaga atgtgcccta
    32401 aggtctggcg taaatgtgca ccggaaccga gaagacccgg attgtcatgg acgatgagat
    32461 acaccggaat atcatggaca tattctttaa agcgcccttt atcttcaaat gcggcacgga
    32521 aaccagaggc tttgaagaac tcaaggaagc gcggcacgat accgccagca atataaaccc
    32581 caccaaaggt tcccagcgtt aatgccagat tgccgccaaa acggcccata atgacgcaaa
    32641 acagcgacaa cgcacggcgg caatcggtgc agctgtcagc cagcgcgcgt tcggtaatat
    32701 cttttggctt gagattttct ggcaggcggt tgtcggcttt aacaattgcg cgatacaaat
    32761 tcaccagccc aggaccagaa agcacgcgtt ccgccgaaac atgaccaatt tccgcacgca
    32821 atatttcgag gataatggcc tcttcttcac tattcggcgc aaaatcaacg tgaccgcctt
    32881 cgcccggcaa gcttacccaa cgcttatcga catggaccag atgcgcaacc ccaagccctg
    32941 ttccggcacc gtaaaccgca ataggtttac cttcgaccgg ttctgcgcca ccaaactgaa
    33001 tcagatgctc ttttttcagc atcgggatcg ccatcgatac agcggtaaaa tcgttaataa
    33061 tttccagatg gctaaaaccg agattctttt tcatttcggc aattgagaac gcccaggtat
    33121 ggttggtcat tgccacccag tcaccggtaa ttgggcaagc gatggcaata cagccgtctt
    33181 tcacctcgac cttatgttct tcaagataaa cgcgaatgac cgcttcgagg ctggggtaat
    33241 caagccctga ataggtctta gcctgcgaga tttcaccact ggcaatatca cacagagcaa
    33301 gacgtgcgtt ggtgccgccc acatcaccga ctaatgcata ctttgtcatt cttcaactgc
    33361 tccgctaaag tcaaaataat tctttctcac actgtaaata cctgggggca taacaacaac
    33421 gccggaaagg caggctccct gtaaatatcg atctgggtca cacatttact ttatcgtttc
    33481 agcaccaatt gcagcgatgc ctttttgcaa gctgggcaaa ctaagtatct gaccccgcat
    33541 aaggaataga acatgctcca tccgcgagcc agaaccatgt tgttattatc gctccccgcc
    33601 gtggcaattg ggattgcgtc cagtcttatt ctgattgtgg tgatgaaaat cgcctcggta
    33661 ttacagaatt tgctctggca acgactgccg ggaactctgg ggatagccca ggattcacct
    33721 atctggatca tcggtgtatt aacgctaacg ggtattgcgg tggggttggt tatccgtttc
    33781 agccagggtc atgccggacc agaccccgcc tgtgaaccgc tgatcggcgc accagttccg
    33841 ccctctgcgc taccaggact tatcgtagca ttaattctcg gtcttgctgg cggcgtcagc
    33901 ctggggccgg aacatccgat catgaccgtc aatatcgccc ttgccgtcgc cattggcgct
    33961 cgtctgttac cgcgcgtcaa ccgaatggag tggactattt tagcctctgc cggaaccatc
    34021 ggcgcgctgt ttggtacacc tgttgcggcg gcgttgatat tttcgcaaac cttaaatggc
    34081 agtagtgaag ttccgctatg ggatcgtctt tttgcgccgt taatggcggc agcagctggt
    34141 gcacttacta ccggattatt tttccatccc catttttcac tgcccattgc gcattacgga
    34201 cagatggaaa tgaccgatat tctcagcggt gcaattgtcg cggcgattgc catcgcagca
    34261 gggatggtcg ccgtatggtg cttaccacgg ttgcacgcga tgatgaatca aatgaaaaat
    34321 ccggtgctcg tgctgggtat tggcggattt attctcggaa ttctgggggt tattggtgga
    34381 ccagtttcgc tgtttaaagg gctggatgag atgcagcaga tggtggcaaa tcaggctttc
    34441 agcaccagcg attacttttt gctggcggta attaaacttg ccgccctggt cgttgctgcc
    34501 gccagtggct ttcgcggtgg gcgaatcttc ccggcagtgt ttgtcggcgt ggcattaggg
    34561 ttgatgctgc atgagcacgt tcccgccgtt ccagcggcaa taaccgtttc ctgcgctatt
    34621 ctcggcatcg tgctggtggt aacacgcgat ggctggttaa gtctttttat ggcggcagtc
    34681 gttgtaccca ataccacatt gctaccgctg ctctgtatcg tcatgcttcc ggcatggctg
    34741 ttattagcag gtaagccgat gatgatggtc aatcgtccga agcaacagcc accccacgat
    34801 aatgtttagc aaaaaatgct ctcctttatg atttaagagt tatggcgatg atacggggct
    34861 ttacctcccc gtaatattgc gttaacaggc cgctgacggc ttcgatcgtg aaggagaata
    34921 acaatgttca ggtcactgtt tctggcggcc gccctgatgg catttacccc gcttgcagca
    34981 aatgcaggtg aaatcaccct actgccatcg atcaaattac aaattggcga tcgcgatcat
    35041 tacggtaatt actgggacgg tggtcactgg cgagatcgtg attactggca tcgcaattat
    35101 gaatggcgca aaaaccgctg gtggcgtcat gataatggct atcatcgtgg ctgggataag
    35161 cgtaaagcat atgagcgtgg ctatcgtgaa ggctggcgcg atcgtgacga tcatcgcgga
    35221 aaaggccgcg gacatgggca ccgccattaa acgcgtcctc caatggagca caatgcctga
    35281 tgcgacgcta gcgcgtctta tcaggcctac aaaccggctc tttaacgcgt agggcggata
    35341 aggcgtttac gccgcatccg ccaatagtgc cagatgcgac gctcattcaa ctacaatccc
    35401 agcgccgtcc ccaccaacaa ccagatattc agcgccacga ccagcactac aatcacccag
    35461 cctgtctgtt ttacgcgttt gctgttcacc agatcgccca tcaacttgct gtcactggtg
    35521 aaaatcagca gtggaaccag cgccagggcg ataccaaaac ttaacagcac ctgactcata
    35581 accagaatcc gtgtcggatc taatcccatc agaatgacaa taaatgacgg caacatggtg
    35641 actgtacgac gcacccacag cgggatatgg aagcgaatga atccctgcat caccacctgc
    35701 cccgccagtg tccccaccac cgttgaggac agtccggcag caaccagact taacccaaag
    35761 accgttgccg cagcatggct taacagcggt tgcagcgtca gataagcctc atcaagatcg
    35821 gcaacaccag tatgaccaga aaagtggaac gccgccgcag ctgtagccat catcgccaga
    35881 ttgacaaaac cggcaatcgt catggcgata gccacatccc atttggtggc ggaataacgt
    35941 tgttgacgcg aaccgccatg taaatgctga gtgagcgaag agtgcaaata aatcacatgc
    36001 ggcataatcg tcgcccctaa cacgcctgct gccaggaaga ccgcctccga agtaggtaaa
    36061 ctcgggatca ccattccttt acccagctgc gccaggttag gctgggagaa aatcaactcg
    36121 acaatgtaag ccgcggcaac aaacaacagt aacccgccaa tcactttctc cagtggtttt
    36181 tgcccgcgac gttgcagcat taaaatcagg aaagtcgcga tccccgtcag caccgcgccc
    36241 tgcaacaacg agacaccaag aatgagttta aaaccgatcg ccgcaccaat aaattccgcc
    36301 aggtcggttg ccatcgcaat aatttctgcc tgaacccaat agaaccacac tacgggacgc
    36361 ggatagtgat cacgaatctg ctccgccaga ttttttccgg tggcaatccc tagtttggca
    36421 gagaggatct gaatcagcat cgccatcagg ttggcccaaa cgacaaccca cagtagctga
    36481 tagccgaagc tggcacccgc ctgaatattg gtcgcaaagt taccgggatc gatataacca
    36541 atcgccgcaa tgaacgcagg tcccattaat gcgagcctca tcttgcgcgc cgcccgtccg
    36601 ctgctactct caacgcgata gttcgtcatc ttgtgcctct aaaacatagc ctttgctatg
    36661 tttcatgcta tgataaacga gaatgattat caaattcatt taaatggatt gtggtgattt
    36721 ctctgataga ccaggattat gactacgaaa agattgatga cttgaatgtt atggttatgt
    36781 ttaatgttag cacatttaca taactttcag cttccataca caacatagca gaaatgtatg
    36841 acagatcact atttttgaag cctgtcacag gacgtcatta tagtgtgtgt cagatctcgt
    36901 tttccttaac catgttacat agaatgtgca cggaaattta acctgcctca tatttggagc
    36961 aaatatggac cgcgtccttc attttgtact ggcacttgcc gttgttgcga ttctcgcact
    37021 gctggtaagc agcgaccgca aaaaaattcg tatccgttat gttattcaac tgcttgttat
    37081 cgaagtgtta ctggcgtggt tcttcctgaa ctccgacgtt ggtttaggct tcgtgaaagg
    37141 cttctccgaa atgttcgaaa aactgctcag atttgccaac gaagggacta acttcgtctt
    37201 tggtagcatg aatgatcaag gcctggcatt cttcttcctg aaagtgctgt gcccaatcgt
    37261 ctttatctct gcactgatcg gtattctcca gcacattcgc gtgttgccgg tgattatccg
    37321 cgcaattggt ttcctgctct ccaaagtcaa cggtatgggc aaactggaat cctttaacgc
    37381 cgtcagctcc ctgattctgg gtcagtctga aaactttatt gcctataaag atatcctcgg
    37441 caaaatctcc cgtaatcgta tgtacaccat ggctgccacg gcaatgtcca ccgtgtcgat
    37501 gtccatcgtt ggtgcataca tgaccatgct ggaaccgaaa tacgtcgttg ctgcgctggt
    37561 actgaacatg ttcagcacct ttatcgtgct gtcgctgatc aatccttacc gtgttgatgc
    37621 cagtgaagaa aacatccaga tgtccaacct gcacgaaggt cagagcttct tcgaaatgct
    37681 gggtgaatac attctggcag gtttcaaagt tgccattatc gttgccgcga tgctgatcgg
    37741 ctttatcgcc ctgatcgccg cgctgaacgc actgtttgcc accgttactg gctggtttgg
    37801 ctacagcatc tccttccagg gcatcctggg ctacatcttc tatccgattg catgggtgat
    37861 gggtgttcct tccagtgaag cactgcaagt gggcagtatc atggcgacca aactggtttc
    37921 caacgagttc gttgcgatga tggatctgca gaaaattgct tccacgctct ctccgcgtgc
    37981 tgaaggcatc atctctgtgt tcctggtttc cttcgctaac ttctcttcaa tcgggattat
    38041 cgcaggtgca gttaaaggcc tgaatgaaga gcaaggtaac gtggtttctc gcttcggtct
    38101 gaaactggtt tacggctcta ccctggtgag tgtgctgtct gcgtcaatcg cagcactggt
    38161 gctgtaaaac catacataaa aaagccgggg atcattcccc ggctttttta cgcctgttaa
    38221 tcagctaatg gctgcgggcg accaatcaaa tacccttgca gatattgcac cccgagctta
    38281 tgcaatagcg cctgctgctg ttgcgtctcg acaaactccg cgaccacact caatgacttc
    38341 gctttcgcca gatcggtaat tgatctcaca atcatcgcat ccagcgtatt cgtgacaata
    38401 tctttcacaa agacgccatc aattttgatg atatcagcct gcaaacgctt taaccgttcg
    38461 tagttggcat atccggtgcc aaagtcatca atcgcaatcc ggaaaccaaa cttatgcagc
    38521 tgctcgatgt tgtacatgct gctttctgcg ttagaaaacg cctgctcctc ggtgatctca
    38581 agaatgaccg cctgcgggga gatgtgataa cgcttaaaca gacgaataat ccgcccggca
    38641 atattctttt gcagcagcgt gagcggcatt aaattgactg aaaagcgcgg tccttttttg
    38701 tcgcaagggt gtgttgccag ccacttcaac aaggattcca gcacttgcaa atcaaaacgc
    38761 gcgctaaggt taaactgagc aataaggggc agaaacttat ccggggtcat aatgccgccg
    38821 tcatatttca gtcgcgcgag gatctcatca taaccttcac cctctttgtt gcgaattggc
    38881 tgggcgtaga gcagcaaatc accctgatct aacgccgtgc gaatggtatt cagcaatagc
    38941 acctgtttag tggtctgccc ggaaaccatc tcctctctgc tatccagcgc cagcacatga
    39001 tgatgtgcgc aggattgctc cgccagccag cttaactgcc ccaacaaggg ttgcagggtt
    39061 tcctgatttc catcaaaacg cccccaggca gcaccatagc ccatatccag cccggtattg
    39121 ttccagtgaa tttgccgact attcaggatg ttaaccatat gctggagtcg cccttccgtt
    39181 tccggcccgc tcagcactaa cagcagttca ctacccggca attgatataa cttttcgttt
    39241 tcctgcatca acggcagcag cgtacggtaa attgatcgga tacagtgaac gcgcatcatt
    39301 aagccgtaat gacgactcat aaactcaaga ttatcaatgc gcaggcagca aaactcttgc
    39361 ccgcctcttg ctccggcgct tgttccaacg cacggaagtt gggcaggagc gttaacggat
    39421 cggtcagcgc ctgcaaatgc cagcggcgat taagccattc actgcgatga taaatgcgca
    39481 ccatatagag caggcaaacg ctaaaggaaa tcagcaccgc aagaataaat gccagcgaat
    39541 attcggtttc caccccttgc aaaaagttct ggttgtaatt cagaaggcaa agcgttgaaa
    39601 cagcccaggt gagatttaaa aacggatagc gaagcttacc gaccccgagg gtaaagatga
    39661 tgaagaaaac gggtaccagg taaccggcaa taaagtcgtt ttcataaggt gtgcacaaca
    39721 gaagtagaag cacgcttaac gctgccagcc agcttaaggt aaatgcgcgt ttctctttgc
    39781 ccaacgacgg cgcgatatcc ctgcgccaca atatctgcgc aaagtgggga cttacaatca
    39841 tgcgagtgag atagtagaaa agcatgttgt aaataagcac agcggtgaaa aggcttagca
    39901 aatcaacgac cgtgaaaatg gcatccgcat cgccgaaaaa ggtggatatc tttagcggga
    39961 aatcaaagaa gctccccaca agatacatgc tgcatttgat gccgatcggc gtcaccaaac
    40021 caagccagaa cagacgttgc cagacatatc ggctggtcaa tccataacgc caacgcgtcc
    40081 ccagctgcca gcgtaatatt gcgcaggcac acaacacgac aaacgtctgg ctaccgagca
    40141 tgaccgcagt ctgtaaaaaa gagagatgaa agttccacag attggtgcaa aacatcccgc
    40201 ataaaacggg aacgacaccg cgccagccaa agataaacaa gattgacagc attacgcaca
    40261 gtggcatcca cgccagaaag atataactgg aatggataat ggcaagtggc gaaataaaac
    40321 gggatagctg aataagtacc acggtgagcg taaacgctag tgtgaatatc ttgatatttt
    40381 ttatcaggtt atgctccaca aacatgagta cactactctc actgatcggg gttttcaggc
    40441 acacaaataa taagtttatt cgcatcgcga agcaaacagg gggccagcct gttgccgatt
    40501 tttcatccaa tgatgctttc accaggcggc actaacccgg acgtagactg tattacaaaa
    40561 gcggcaaaaa gcagagacaa aaaacccccg ctttgcagcg agggttggaa atttggtgga
    40621 gctaagcggg atcgaaccgc tgacctcttg catgccatgc aagcgctctc ccagctgagc
    40681 tatagcccca cgatgcgttt acgtaccaaa tttgctgggt gcaaaatttg gtggagctaa
    40741 gcgggatcga accgctgacc tcttgcatgc catgcaagcg ctctcccagc tgagctatag
    40801 ccccgtcacg taaagcttgt cgagttgacg ggcggcatca tatgaattcc gcccgaatgt
    40861 gtcaacggca aattgcaacg tgtagtttca atcgctgaaa aatcaggcaa atgaacaatt
    40921 ttggaaaacc gctcgcattc agtagttatt catgtcacgg tttcctgtaa agtggtgtta
    40981 taaaatgaac tactaataga cccacataca ttcagggaat tgttatgttc aaggagagga
    41041 tgacaccaga tgaacttgcc agactgaccg gctatagccg ccagaccatt aataaatggg
    41101 tacgcaagga gggctggaca acgtcaccaa aacctggcgt ccagggtggc aaagcccgac
    41161 tggttcacgt caatgaacag gttcgtgaat atattcgcaa tgctgaacgt ccagaaggtc
    41221 agggagaagc gcctgccctt tctggtgatg cgccgcttga agtattgctg gtgacactgg
    41281 cgaaagagat gacgccagtt gaacaaaaac agtttacatc cttgcttctg cgggaaggga
    41341 ttatcggatt gttacaacgc ttagggattc gcgatagcaa ataatatgaa aagattacgc
    41401 aataaaatga ccaccgaaga actggctgaa tgcctcggtg tggctaaaca aactgttaac
    41461 cgttggatca gagaaaaagg ctggaaaacg gaaaaatttc ccggcgtgaa aggcggtcgt
    41521 gccagactta ttctggtcga tacgcaagtt tgcgagttta ttcagaacac gccagccttc
    41581 cataacacac caatgttgat ggaagctgaa gagagaattg ctgaatacgc tcctggagca
    41641 cgcgccccgg cgtatcgaca aatcatcaac gcgatagata atatgacaga cattgagcag
    41701 ggaaaagtcg cgcaattttt gtcacgtgaa ggaattcgca atttcctcgc ccgtctcgat
    41761 attgacgaat cagcataaat aaaaaacggc aggatattat ctcctgccgt ttatcttttt
    41821 acacgctaat tactgctgat tttcgcgttc agcaataaaa tccagcgctt tgttgatacg
    41881 ctcgatactg cgggtcttac caattgcgtg aacggtaaca tccagtgctg gagactgccc
    41941 cgccccggtt acggcgacac gcagcggcat accaacttta cccataccca cttccagctc
    42001 atccgccgtc gcctgaatag cgtgatgaac gttttcagcg gtccagtcag taatcgcggc
    42061 cagtttgtca cgaaccactt ccagcggctg acgcgctacc ggacgcagat gttttttcgc
    42121 cgcatcggca tcgaactcag caaaatcttc gtagaaataa cggcagctct gtgccatctc
    42181 tttcagcgtc ttgcagcgtt cgcccagcag tttcaccaga tcagccagct gcgggccgtt
    42241 acgggtatcg atattttcct gctcaatgtg ccactgtaag tgagtagcaa catactccgg
    42301 cggcagcgcg ttaatgtagt gatggttcag ccacagcagc ttgtcggtgt tgaacgcact
    42361 ggcagatttg ctgacggcat tcaaagtgaa gtatttgatc atctcttcac gagtgaagat
    42421 ttcctgatcg ccgtgggacc agcccagacg caccagatag ttcagcagtg cttctggcaa
    42481 ataaccgtca tcacgatact gcattacgct gactgccccg tgacgtttgg acagtttttt
    42541 accgtcatcg ccattgatca tagaaacgtg cgcgtaaacc ggcaccggcg ctttcagggc
    42601 cttaagaatg ttgatctggc gcggcgtgtt gttgatatgg tcttcgccac ggataacgtg
    42661 ggtgatttcc atatcccagt catcgacaac cacacagaag ttataggttg gggaaccatc
    42721 ggtacggcgg ataataagat cgtccagttc ctggttgctg aactcgatcg gaccacggat
    42781 ctgatcgtca aaaacaacag aaccttcctg cgggttagca aaacgtacaa cacacggttc
    42841 atcatcagca tgatgctcat ggctgtggcg gcagcgaccg tcataacgcg gcttctcacc
    42901 tttcgccatt tgctcttcgc gcagcgcttc caggcgctct ttagagcaat agcatttata
    42961 agcagtgccc tcttccagca tctgatcgat caccgcgttg tagcgatcaa aacgtttggt
    43021 ctggtagtac ggaccttcat cccactccag gctcagccag ttcatgccat ccataatggc
    43081 ttcgatagct tccggcgtgg aacgctcaag atcggtgtct tcaatacgca gcacgaactc
    43141 accgccgtgg ttacgtgcaa aaagccagga gtaaagagca gtacgcgcgc cgccaacgtg
    43201 cagatagcct gttgggcttg gcgcgaagcg agttttgatt ttcatgaaat ggccttacgt
    43261 ttagaaagat gccgacaacc ggcaaatcct ggaaaaattg agtgggcgat attctatcac
    43321 tcccgcctga ttcctcaatg tagttcgggt ttctacctta cgcattattg ttttcgttga
    43381 caaattgcgc aacacggtct gttttgcgat catcttgctt aaatttacga cgaacgaaca
    43441 atttctttag aaaatgcgtt gactcatttt gaactctccc tataatgcgg ctccacacag
    43501 cgggggtgat tagctcagct gggagagcac ctcccttaca aggagggggt cggcggttcg
    43561 atcccgtcat cacccaccaa ctactttatg tagtctccgc cgtgtagcaa gaaattgaga
    43621 agtgggtgat tagctcagct gggagagcac ctcccttaca aggagggggt cggcggttcg
    43681 atcccgtcat cacccaccaa tttctcgcca gctaaatttc ttgtaaaaat gtgaagtacc
    43741 gaagtgggtg attagctcag ctgggagagc acctccctta caaggagggg gtcggcggtt
    43801 cgatcccgtc atcacccacc acttcgggtc gttagctcag ttggtagagc agttgacttt
    43861 taatcaattg gtcgcaggtt cgaatcctgc acgacccacc aatgtaaaaa agcgccctaa
    43921 aggcgctttt ttgctatctg cgatactcaa agattcgaac ctgcagcagg tttgagttga
    43981 gcgcagcgaa acaacggagc cgctcgcggc gacggcccga agggcgagcg aagcgagtca
    44041 tcctgcacga cccaccaatg taaaaaagcg ccctaaaggc gcttttttgc tatctgcgat
    44101 ttgtaagatt gatttttctt ctcaagttcc ttgttcttct gccgatatta cctcgtacag
    44161 tgacgaggac tgaacaatgg ccactatctc ttcaacttca attccttcca tacaaacaca
    44221 gagtagtaac agggcatcac aaggtagcga tgttgcttca aaaatcgcgc ggataagtca
    44281 gcaaatcatc aaactcactc aacaaattaa agagattgta gacactagcg gcagtgctga
    44341 agacaaacaa aaacaggccg aattgattca acagcaaatc accctactgg aaacacaact
    44401 ggcgcagttg cagagacagc aggcagaaaa ggcgcaggag aaagagcagc gtttgtctct
    44461 gaatgtatcc cttctaaatc ctgttgaaaa tacgactcat atcgatatct atatctgata
    44521 caacggagcc ccttcccaac cagccattag cgcattgccc tggtctcgta ccacgtccca
    44581 taacgcttca gcggcggtgg aaagtgaacg gttcttcctg cgcaccagca tcaactggcg
    44641 ttcaacaacc ggcgtaatgc gctttaccac cagtgggcta ccttcaggca atggtagcgc
    44701 cagtgcggga agaatactga tgccgatgcc tgcggctacc atcgggaaca gcgtcgccgg
    44761 atgtccaatc tcctgcacaa tattcgcctg aataccattg cgcgccagcg ctgcgtcaat
    44821 cagcggtcgg ctgcctgacg cgtaatcctg caacaccaat ttcgctccct gtaatgcctg
    44881 ccagggtaca taatcttcca cggccaaagc gctatcgcga tggcaaagca gaaagaaagg
    44941 ctcggaaaga atcgcttcac attgcagatc gcccactggg cctggatcga tgacgatgca
    45001 aaaatcgaca tctccctggc gaatactttc catcacccac tgctgcggac gatcgtgcag
    45061 gacaaactga atatctggat agcggcgatg gctttcagca atgcattgcg gaataagatg
    45121 cgcggaaatg gtctggctgg cagcgacccg aacttttccg ctcagttgtt gccccatacg
    45181 cccggtatcg cgcaacgtgc tgttaagttc atccagcagt cgctcaagac gcaaagccaa
    45241 ctgctgccct gcatctgtaa gcaccacttc acgcgtggtt ctgtccagca ggcgaacacc
    45301 agtatgattt tccagctcct tcacactgtg acttactgcc gactggctca agccgatacg
    45361 ctctcctgca cgactaaaac ttttctcctg cgctactgtg acgaaaacct ttaattgttt
    45421 taaagaataa ttcatctgtt ttcttcatga atggatgcaa taaatcaatt ttatttctca
    45481 aactggaaga agcacaatag aaccatcgat catctggagt ctttatgaaa ctttttcgta
    45541 tcctcgatcc tttcacctta accctgatca cggtggtgtt gctggcctct ttctttccgg
    45601 ccagaggcga tttcgtcccc ttctttgaaa atctgaccac cgcagctatt gccctgctgt
    45661 tctttatgca cggcgcgaag ttgtcgcgtg aggcgattat tgctggcggt ggtcactggc
    45721 gactgcattt gtgggtaatg tgcagcacct tcgtgctgtt tccgattctg ggtgtactgt
    45781 ttgcctggtg gaaaccggta aatgtcgacc cgatgctcta ctccggtttt ctctacttgt
    45841 gcattctccc ggctaccgtg cagtctgcaa tcgccttcac gtcaatggcg ggcggtaacg
    45901 tcgcggcggc ggtttgttct gcgtcagcat ccagcctgct ggggattttc ctttcaccat
    45961 tgctagttgg tctggtgatg aatgttcacg gtgcaggggg cagccttgag caggtcggta
    46021 aaattatgct gcaactgctg ctgccgtttg tgttggggca tctttcccgg ccgtggattg
    46081 gtgactgggt gtcgcgcaat aaaaaatgga ttgcgaaaac tgaccagacg tccattctgt
    46141 tggtggttta tacagcgttc agcgaagccg tcgttaatgg tatctggcat aaagttggct
    46201 ggggatcatt gctgtttatc gtggtggtca gctgcgttct tctggctatc gtgattgtag
    46261 ttaacgtctt tatggcacgc cgactgggct tcaataaggc agatgaaatt actatcgtct
    46321 tttgtggttc gaaaaagagt ctggcaaatg gcatcccgat ggcaaacatt ctgttcccca
    46381 catcggtgat cggtatgatg gtgctgcccc tgatgatttt ccatcagatc caattgatgg
    46441 tctgtgcggt gctggcgcgt cgatacaaac gccagaccga acagttacag gcgcagcagg
    46501 aaagcagcgc cgataaagct taaagcggac gcttcagggg ctggaccagt tgcgtcagcc
    46561 cctcggtttt aatcagcagc gtgatttgca ttagctcacc gagcttccct gccgggaact
    46621 catctttgcg ggcaaaccac agcagatact cctccggcag gtcgattaag cgacgccctt
    46681 tgtatttgcc aaacggcatt atcgtattgg ctatttcaat cagctgctct ttttccatct
    46741 cagctaccca gcaaacgcag catttccgct tcgtcgatga cttcaatgcc cagttcctgc
    46801 gccttcgcca gtttagatcc tgcagcttca cccgctatca ccagatcggt tttcttcgac
    46861 acgctgcccg cgactttcgc gcccagttcg accagtcgag ctttagcgtc atcacgcgac
    46921 atctggctta agctgcccgt aagcaccacg gttttaccag caaacgggct gtcaatctct
    46981 tccgcgttga taacgatcgg cgcaggccag tgaacacctt ccgccaacag ctcgctgatg
    47041 acattgcggt tgctttcttc ggcaaagaag ttgtgaacgt gggatgcaac gacaatgcca
    47101 acatcaggca ccttttgcag ctcttcaatc gaagcggctt ccagcacttc cagcgtgccg
    47161 aaatatgccg ccagacctgc tgcggtggcc tcgccgactt cacggatgcc aagtgcatag
    47221 aggaagcgag caaaggtggt ttctttcgct ttttccagcg cgttaaccac gttttgtgcc
    47281 gattttggcc ccatacgctc cagtccggtc agtttgcctg cggtgagttt gaacagatct
    47341 gccggagtgt ggacatattc tttttcaacc agctgatcga tgattttgtc gcccattccg
    47401 tcaacatcca tcgcacggcg ggaaacaaag tgtttcagcg actctttacg ctgcgcaccg
    47461 caaatcaggc caccggtaca gcgggcaacc gcttcacctt ccacacgctc aacgtcagaa
    47521 ccacataccg gacaatgcgt cgggaataca acctcacggg tatcttccgg gcgttcagaa
    47581 agcacgacgt taaccacctg cgggatcacg tcgccagcgc ggcgaatcac cactttatcg
    47641 ccaatgcgta aaccaagacg ttcgatttca tccgcattgt gtaaggttgc gttactcacc
    47701 agcacgccag caacatggac aggttccaga cgcgcaacag gcgtaatcgc gccagtacgc
    47761 ccaacctgaa actcgacgtc acgcacaaag gtcatctgct cctgcgccgg gaatttaaac
    47821 gctaccgccc agcgcggggc acgcgcgaca aagccaagct gctcctgctg ttccagtgag
    47881 ttgaccttaa tcaccacgcc gtcgatatca aagcccagcg tcgggcggtc ttcttccact
    47941 ttgtgataga acgccagcac ttcttccgcc gattcacaaa gcgttacccg atcgctgacc
    48001 ggcaaccccc actttttaaa ttgcagtaaa cggccaagat gagtatccgg cagctcgcca
    48061 ccttccagaa caccaacgcc atagcagaaa aaagtgagcg gtcgcttcgc tgtaatacgc
    48121 ggatcaagct gacgcagtga accagctgcc gcattacgtg ggttagcaaa aactttcccg
    48181 cccgtgcgtc gcgcatcttc gttaatcttt tcgaaccccg cctgcggcag gaacacttca
    48241 ccacgcactt ccagacgcgc cgggatattc tctccgtgca gcttcagcgg aatggcgcga
    48301 atagtacgca cattagacgt gatatcttcc ccggtggtgc catcgccacg ggtcgcggca
    48361 ctgactaaaa cgccattttc atacagaata ctgacggcaa ggccatccag cttcagctca
    48421 cagcaccagg tgactttctc gttgtttttc agacggtcct gcacacgttt gttgaaagca
    48481 agaaagcttt cttcatcaaa aacgttatcc agtgacagca ttggtacttc atggcgtatc
    48541 tggctgaaag ccgccagcgg cgcagcacct acacgttggg taggcgaatc aggcgtaatc
    48601 agttctggat gtttggtttc cagctcgcgc agttcgcgca tcagcctgtc gtattcagcg
    48661 tcgggaattt ccggcgcatc catcacatga taaagatatt catgatggcg aagcgtcgtt
    48721 cgcagttctg tcagttgttg ttcgattgat tccatatcgc accatcaatg ctaaaaaccc
    48781 ccgacaagcg ggggttcgaa gaggagttaa tttgccttaa gtgtatcagg cgttggcgtc
    48841 tttgacttcg cggatgatgt cctggtactc gcgcaatttc tgcggagtca tcatacggcg
    48901 ctgatcgtca agcacgacac cgcccacttc atcggcaata tgctgcgcag attgcagcat
    48961 cagcttgaag ttctgcagct cgtcaccgta agacggtacc tgcataaaga tagtgacacc
    49021 cggagtagtg aaatccttca tttcaggatc aaaggttccc ggtttcacca tattcgccag
    49081 gctgaataac gccgggccgc tgccatccgg gctaagatga cgatggtaaa tattcatatc
    49141 gccaaaaatg aagcccgctt gttgaatgct gttaagaagc agttcaccgt ttagctcgct
    49201 accgtgatgc gccgcgacgt tcatgataat caccgcttct ttgcgcttcg gtttatccat
    49261 aactggagcc ggttccgcta caggctcagg ctgtggtgcc gctacgggtt ctgcaggctg
    49321 gaaagcctgt tgtgccggtt gcggtgctga atgcacaggc tgcggcgctg gcgcgacctg
    49381 tggcgaaact ggctgctgca acggctgttc aggctgcggc tgataggcag gctgctgcat
    49441 cggctgcgct ggacgcggag catgttgcgg cggtacctgc gcttcaggcg gctgctggat
    49501 cggttgacgc ggctgcgcag acgcataagg cggttggtac tggtgttgcg gcgacggacg
    49561 agcagcctca tgctcctgag cgttagccgg ggcatgattc acgcggtgaa cacgaacctc
    49621 accaacgccc tcatcatctt cgacatcctc gtcataagaa tcgtcgtcac gttttgactt
    49681 cattcgtttt aatggccgat cgcggaacat agaagatcgt tctttacggc tggtccagaa
    49741 accatgtacc agtaaagcga ttatggcgat cgcgccaaca atgattaata tcagacgcaa
    49801 atcctgcatc attatattct ctgttgttct aacaccttgc caccacggca aacatttact
    49861 cactaagagt atttgccgat tacctcaagt gcaagtgcac tattaacttt cacagcacaa
    49921 agatagatga aatcgtgctt tttgctgttt tttcgaacat atcctaactg tccattgcgc
    49981 aattacccgg taaaatacgc agaattttcc tgggattggt caaaaggagc tcatcctgat
    50041 tatggtttca tcattcacat ctgccccacg cagcggtttt tactattttg cgcaaggctg
    50101 gaagctcgtc tcgcaacctg ggattcggcg tttcgttatt ttaccgctgc tggtcaatat
    50161 tttgttgatg gggggcgcat tctggtggct ctttacacag ctcgatgtct ggatcccgac
    50221 tctcatgagt tacgttccgg actggctaca atggctgagt tatctgttgt ggcctctggc
    50281 tgtcatctct gtgctgttag tgtttggcta tttcttctcc acgattgcta actggattgc
    50341 cgctccgttt aacggtttat tggctgaaca actggaagca cgattgactg gcgctacacc
    50401 gccagatacc gggattttcg gtatcatgaa agatgtgccg cgaatcatga aacgcgaatg
    50461 gcaaaaattt gcctggtatc tgccgcgcgc aattgtatta ctaattcttt acttcatccc
    50521 tggtattggg caaaccgtcg cgccggtact gtggttcctg tttagcgcct ggatgttagc
    50581 catccagtat tgcgattacc ccttcgataa ccacaaagtg ccgtttaaag agatgcgcac
    50641 cgccctgcgc acacgcaaaa tcaccaatat gcagtttggt gctttaacca gcctgtttac
    50701 gatgatcccg ctgcttaatc tgttcatcat gcccgttgcc gtttgtggcg cgacggcgat
    50761 gtgggtcgat tgctatcgcg ataaacacgc gatgtggcgg taacaatcta ccggttattt
    50821 tgtaaaccgt ttgtgtgaaa caggggtggc ttatgccgcc ccttattcca tcttgcatgt
    50881 cattatttcc cttctgtata tagatatgct aaatccttac ttccgcatat tctctgagcg
    50941 ggtatgctac ctgttgtatc ccaatttcat acagttaagg acaggccatg agtaagattt
    51001 ttgaagataa ctcgctgact atcggtcaca cgccgctggt tcgcctgaat cgcatcggta
    51061 acggacgcat tctggcgaag gtggaatctc gtaaccccag cttcagcgtt aagtgccgta
    51121 tcggtgccaa catgatttgg gatgccgaaa agcgcggcgt gctgaaacca ggcgttgaac
    51181 tggttgaacc gaccagcggt aataccggga ttgcactggc ctatgtagct gccgctcgcg
    51241 gttacaaact caccctgacc atgccagaaa ccatgagtat tgaacgccgc aagctgctga
    51301 aagcgttagg tgcaaacctg gtgctgacgg aaggtgctaa aggcatgaaa ggcgcaatcc
    51361 aaaaagcaga agaaattgtc gccagcaatc cagagaaata cctgctgctg caacaattca
    51421 gcaatccggc aaaccctgaa attcacgaaa aaaccaccgg tccggagatc tgggaagata
    51481 ccgacggtca ggttgatgta tttattgctg gcgttgggac tggcggtacg ctgactggcg
    51541 tcagccgcta cattaaaggc accaaaggca agaccgatct tatctctgtc gccgttgagc
    51601 caaccgattc tccagttatc gcccaggcgc tggcaggtga agagattaaa cctggcccgc
    51661 ataaaattca gggtattggc gctggtttta tcccggctaa cctcgatctc aagctggtcg
    51721 ataaagtcat tggcatcacc aatgaagaag ccatttctac cgcccgtcgt ctaatggaag
    51781 aagaaggtat tcttgcaggt atctcttctg gagcagctgt tgccgcggcg ttgaaactac
    51841 aagaagatga aagctttacc aataagaata ttgtggttat tctaccatca tcgggtgagc
    51901 gttatttaag caccgcattg tttgccgatc tcttcactga gaaagaattg caacagtaat
    51961 gccagcttgt taaaaatgcg taaaaaagca cctttttagg tgcttttttg tggcctgctt
    52021 caaactttcg cccctcctgg cattgattca gcctgtcgga actggtattt aaccagacta
    52081 attattttga tgcgcgaaat taatcgttac aggaaaagcc aaagctgaat cgattttatg
    52141 atttggttca attcttcctt taacggcata atgtttaatg acgtacgaaa cgtcagcggt
    52201 caacacccgc cagcaatgga ctgtattgcg ctcttcgtgc gtcgcgtctg ttaaaaactg
    52261 gcgctaacaa tacaggctaa agtcgaaccg ccaggctaga ctttagttcc acaacactaa
    52321 acctataagt tggggaaata caatgttcca gcaagaagtt accattaccg ctccgaacgg
    52381 tctgcacacc cgccctgctg cccagtttgt aaaagaagct aagggcttca cttctgaaat
    52441 tactgtgact tccaacggca aaagcgccag cgcgaaaagc ctgtttaaac tgcagactct
    52501 gggcctgact caaggtaccg ttgtgactat ctccgcagaa ggcgaagacg agcagaaagc
    52561 ggttgaacat ctggttaaac tgatggcgga actcgagtaa tttcccgggt tcttttaaaa
    52621 atcagtcaca agtaaggtag ggttatgatt tcaggcattt tagcatcccc gggtatcgct
    52681 ttcggtaaag ctctgcttct gaaagaagac gaaattgtca ttgaccggaa aaaaatttct
    52741 gccgaccagg ttgatcagga agttgaacgt tttctgagcg gtcgtgccaa ggcatcagcc
    52801 cagctggaaa cgatcaaaac gaaagctggt gaaacgttcg gtgaagaaaa agaagccatc
    52861 tttgaagggc atattatgct gctcgaagat gaggagctgg agcaggaaat catagccctg
    52921 attaaagata aacacatgac agctgacgca gctgctcatg aagttatcga aggtcaggct
    52981 tctgccctgg aagagctgga tgatgaatac ctgaaagaac gtgcggctga cgtacgtgat
    53041 atcggtaagc gcctgctgcg caacatcctg ggcctgaaga ttatcgacct gagcgccatt
    53101 caggatgaag tcattctggt tgccgctgac ctgacgccgt ccgaaaccgc acagctgaac
    53161 ctgaagaagg tgctgggttt catcaccgac gcgggtggcc gtacttccca cacctctatc
    53221 atggcgcgtt ctctggaact gcctgctatc gtgggtaccg gtagcgtcac ctctcaggtg
    53281 aaaaatgacg actatctgat tctggatgcc gtaaataatc aggtttacgt caatccaacc
    53341 aacgaagtta ttgataaaat gcgcgctgtt caggagcaag tggcttctga aaaagcagag
    53401 cttgctaaac tgaaagatct gccagctatt acgctggacg gtcaccaggt agaagtatgc
    53461 gctaacattg gtacggttcg tgacgttgaa ggtgcagagc gtaacggcgc tgaaggcgtt
    53521 ggtctgtatc gtactgagtt cctgttcatg gaccgcgacg cgctgcccac tgaagaagaa
    53581 cagtttgctg cttacaaagc agtggctgaa gcgtgtggct cgcaggcggt tatcgttcgt
    53641 accatggaca tcggcggcga caaagagctg ccatacatga acttcccgaa agaagagaac
    53701 ccgttcctcg gctggcgcgc tatccgtatc gcgatggatc gtaaagagat cctgcgcgat
    53761 cagctccgcg ctatcctgcg tgcctcggct ttcggtaaat tgcgcattat gttcccgatg
    53821 atcatctctg ttgaagaagt gcgtgcactg cgcaaagaga tcgaaatcta caaacaggaa
    53881 ctgcgcgacg aaggtaaagc gtttgacgag tcaattgaaa tcggcgtaat ggtggaaaca
    53941 ccggctgccg caacaattgc acgtcattta gccaaagaag ttgatttctt tagtatcggc
    54001 accaatgatt taacgcagta cactctggca gttgaccgtg gtaatgatat gatttcacac
    54061 ctttaccagc caatgtcacc gtccgtgctg aacttgatca agcaagttat tgatgcttct
    54121 catgctgaag gcaaatggac tggcatgtgt ggtgagcttg ctggcgatga acgtgctaca
    54181 cttctgttgc tggggatggg tctggacgaa ttctctatga gcgccatttc tatcccgcgc
    54241 attaagaaga ttatccgtaa cacgaacttc gaagatgcga aggtgttagc agagcaggct
    54301 cttgctcaac cgacaacgga cgagttaatg acgctggtta acaagttcat tgaagaaaaa
    54361 acaatctgct aatccacgag atgcggccca atttactgct taggagaaga tcatgggttt
    54421 gttcgataaa ctgaaatctc tggtttccga cgacaagaag gataccggaa ctattgagat
    54481 cattgctccg ctctctggcg agatcgtcaa tatcgaagac gtgccggatg tcgtttttgc
    54541 ggaaaaaatc gttggtgatg gtattgctat caaaccaacg ggtaacaaaa tggtcgcgcc
    54601 tgtagacggt accattggta aaatctttga aaccaatcac gcgttctcta tcgaatctga
    54661 tagcggcgtt gaactgttcg tccacttcgg tatcgacacc gttgaactga aaggcgaagg
    54721 cttcaagcgt attgctgaag aaggtcagcg cgtgaaagtt ggcgatactg tcattgaatt
    54781 tgatctgccg ctgctggaag agaaagccaa gtctaccctg actccggttg ttatctccaa
    54841 catggacgaa atcaaagaac tgatcaaact gtccggtagc gtaaccgtgg gtgaaacccc
    54901 ggttatccgc atcaagaagt aattcttgcc gcagtgaaaa atggcgccca
//
LOCUS       DAESHI010000017        53404 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.017, whole genome shotgun sequence.
ACCESSION   DAESHI010000017 DAESHI010000000
VERSION     DAESHI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.017"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..852)
                     /gene="melB"
                     /locus_tag="KW011_001039"
     CDS             complement(<1..852)
                     /gene="melB"
                     /locus_tag="KW011_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HBH7659442.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLV"
     gene            complement(955..2310)
                     /gene="melA"
                     /locus_tag="KW011_001040"
     CDS             complement(955..2310)
                     /gene="melA"
                     /locus_tag="KW011_001040"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBH7659443.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2593..3501
                     /gene="melR"
                     /locus_tag="KW011_001041"
     CDS             2593..3501
                     /gene="melR"
                     /locus_tag="KW011_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HBH7659444.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3700..5967
                     /gene="adiA"
                     /locus_tag="KW011_001042"
     CDS             3700..5967
                     /gene="adiA"
                     /locus_tag="KW011_001042"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HBH7659445.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            6292..7053
                     /gene="adiY"
                     /locus_tag="KW011_001043"
     CDS             6292..7053
                     /gene="adiY"
                     /locus_tag="KW011_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HBH7659446.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            7190..8527
                     /gene="adiC"
                     /locus_tag="KW011_001044"
     CDS             7190..8527
                     /gene="adiC"
                     /locus_tag="KW011_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HBH7659447.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            8631..10274
                     /gene="eptA"
                     /locus_tag="KW011_001045"
     CDS             8631..10274
                     /gene="eptA"
                     /locus_tag="KW011_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HBH7659448.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            10271..10939
                     /gene="basR"
                     /locus_tag="KW011_001046"
     CDS             10271..10939
                     /gene="basR"
                     /locus_tag="KW011_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HBH7659449.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            10940..12040
                     /gene="pmrB"
                     /locus_tag="KW011_001047"
     CDS             10940..12040
                     /gene="pmrB"
                     /locus_tag="KW011_001047"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HBH7659450.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(12016..12105)
                     /gene="pmrR"
                     /locus_tag="KW011_001048"
     CDS             complement(12016..12105)
                     /gene="pmrR"
                     /locus_tag="KW011_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HBH7659451.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(12217..13719)
                     /gene="proP"
                     /locus_tag="KW011_001049"
     CDS             complement(12217..13719)
                     /gene="proP"
                     /locus_tag="KW011_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HBH7659452.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(13983..14849)
                     /locus_tag="KW011_001050"
     CDS             complement(13983..14849)
                     /locus_tag="KW011_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HBH7659453.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(14846..17074)
                     /gene="crfC"
                     /locus_tag="KW011_001051"
     CDS             complement(14846..17074)
                     /gene="crfC"
                     /locus_tag="KW011_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HBH7659454.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            17476..17811
                     /locus_tag="KW011_001052"
     CDS             17476..17811
                     /locus_tag="KW011_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HBH7659455.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            17969..18412
                     /gene="yjdN"
                     /locus_tag="KW011_001053"
                     /pseudo
     CDS             17969..18412
                     /gene="yjdN"
                     /locus_tag="KW011_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            18545..19333
                     /gene="phnC"
                     /locus_tag="KW011_001054"
     CDS             18545..19333
                     /gene="phnC"
                     /locus_tag="KW011_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HBH7659456.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESAGIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            19358..20374
                     /gene="phnD"
                     /locus_tag="KW011_001055"
     CDS             19358..20374
                     /gene="phnD"
                     /locus_tag="KW011_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH7659457.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            20500..21279
                     /gene="phnE"
                     /locus_tag="KW011_001056"
     CDS             20500..21279
                     /gene="phnE"
                     /locus_tag="KW011_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HBH7659458.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            21300..22025
                     /gene="phnF"
                     /locus_tag="KW011_001057"
     CDS             21300..22025
                     /gene="phnF"
                     /locus_tag="KW011_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HBH7659459.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            22026..22478
                     /gene="phnG"
                     /locus_tag="KW011_001058"
     CDS             22026..22478
                     /gene="phnG"
                     /locus_tag="KW011_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBH7659460.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            22475..23059
                     /gene="phnH"
                     /locus_tag="KW011_001059"
     CDS             22475..23059
                     /gene="phnH"
                     /locus_tag="KW011_001059"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBH7659461.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            23059..24123
                     /gene="phnI"
                     /locus_tag="KW011_001060"
     CDS             23059..24123
                     /gene="phnI"
                     /locus_tag="KW011_001060"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HBH7659462.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            24116..24961
                     /gene="phnJ"
                     /locus_tag="KW011_001061"
     CDS             24116..24961
                     /gene="phnJ"
                     /locus_tag="KW011_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBH7659463.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            24958..25716
                     /gene="phnK"
                     /locus_tag="KW011_001062"
     CDS             24958..25716
                     /gene="phnK"
                     /locus_tag="KW011_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HBH7659464.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            25827..26507
                     /gene="phnL"
                     /locus_tag="KW011_001063"
     CDS             25827..26507
                     /gene="phnL"
                     /locus_tag="KW011_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HBH7659465.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            26504..27640
                     /gene="phnM"
                     /locus_tag="KW011_001064"
     CDS             26504..27640
                     /gene="phnM"
                     /locus_tag="KW011_001064"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBH7659466.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLVNLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            27640..28197
                     /gene="phnN"
                     /locus_tag="KW011_001065"
     CDS             27640..28197
                     /gene="phnN"
                     /locus_tag="KW011_001065"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HBH7659467.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            28184..28618
                     /gene="phnO"
                     /locus_tag="KW011_001066"
     CDS             28184..28618
                     /gene="phnO"
                     /locus_tag="KW011_001066"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HBH7659468.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            28620..29378
                     /gene="phnP"
                     /locus_tag="KW011_001067"
     CDS             28620..29378
                     /gene="phnP"
                     /locus_tag="KW011_001067"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HBH7659469.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPIYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(29525..29854)
                     /gene="yjdP"
                     /locus_tag="KW011_001068"
     CDS             complement(29525..29854)
                     /gene="yjdP"
                     /locus_tag="KW011_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HBH7659470.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(29923..30240)
                     /locus_tag="KW011_001069"
     CDS             complement(29923..30240)
                     /locus_tag="KW011_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HBH7659471.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            30493..32478
                     /gene="yjcS"
                     /locus_tag="KW011_001070"
     CDS             30493..32478
                     /gene="yjcS"
                     /locus_tag="KW011_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HBH7659472.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            32687..32962
                     /locus_tag="KW011_001071"
     CDS             32687..32962
                     /locus_tag="KW011_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659473.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            32981..34012
                     /gene="mdtN"
                     /locus_tag="KW011_001072"
     CDS             32981..34012
                     /gene="mdtN"
                     /locus_tag="KW011_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HBH7659474.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            34012..36063
                     /gene="mdtO"
                     /locus_tag="KW011_001073"
     CDS             34012..36063
                     /gene="mdtO"
                     /locus_tag="KW011_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HBH7659475.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            36060..37526
                     /gene="mdtP"
                     /locus_tag="KW011_001074"
                     /pseudo
     CDS             36060..37526
                     /gene="mdtP"
                     /locus_tag="KW011_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
     gene            37724..39871
                     /gene="fdhF"
                     /locus_tag="KW011_001075"
     CDS             37724..39871
                     /gene="fdhF"
                     /locus_tag="KW011_001075"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:38141..38143,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBH7659476.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMDHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            39965..40654
                     /locus_tag="KW011_001076"
     CDS             39965..40654
                     /locus_tag="KW011_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBH7659477.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(40733..42046)
                     /gene="gltP"
                     /locus_tag="KW011_001077"
     CDS             complement(40733..42046)
                     /gene="gltP"
                     /locus_tag="KW011_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HBH7659478.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHRELLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(42388..42984)
                     /gene="nrfG"
                     /locus_tag="KW011_001078"
     CDS             complement(42388..42984)
                     /gene="nrfG"
                     /locus_tag="KW011_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HBH7659479.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(42981..43364)
                     /gene="nrfF"
                     /locus_tag="KW011_001079"
     CDS             complement(42981..43364)
                     /gene="nrfF"
                     /locus_tag="KW011_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HBH7659480.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(43357..45075)
                     /locus_tag="KW011_001080"
     CDS             complement(43357..45075)
                     /locus_tag="KW011_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HBH7659481.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(45095..46051)
                     /gene="nrfD"
                     /locus_tag="KW011_001081"
     CDS             complement(45095..46051)
                     /gene="nrfD"
                     /locus_tag="KW011_001081"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HBH7659482.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(46048..46719)
                     /gene="nrfC"
                     /locus_tag="KW011_001082"
     CDS             complement(46048..46719)
                     /gene="nrfC"
                     /locus_tag="KW011_001082"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HBH7659483.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(46716..47282)
                     /gene="nrfB"
                     /locus_tag="KW011_001083"
     CDS             complement(46716..47282)
                     /gene="nrfB"
                     /locus_tag="KW011_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HBH7659484.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(47327..48763)
                     /gene="nrfA"
                     /locus_tag="KW011_001084"
     CDS             complement(47327..48763)
                     /gene="nrfA"
                     /locus_tag="KW011_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HBH7659485.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            49157..51115
                     /gene="acs"
                     /locus_tag="KW011_001085"
     CDS             49157..51115
                     /gene="acs"
                     /locus_tag="KW011_001085"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HBH7659486.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLTCGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            51315..51628
                     /locus_tag="KW011_001086"
                     /pseudo
     CDS             51315..51628
                     /locus_tag="KW011_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            51625..53274
                     /gene="actP"
                     /locus_tag="KW011_001087"
     CDS             51625..53274
                     /gene="actP"
                     /locus_tag="KW011_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HBH7659487.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
BASE COUNT        12839 a        14704 c        13890 g        11971 t
ORIGIN      
        1 tactaacgtc accaggttag cagctcccgc atacgacaga taatagggga acaaatccgc
       61 atcaccgata acatatgaga aataatagat agcaaatccg gtaataatgt tgctggctac
      121 attataagca agagccatac ccaagaggca tgaaagctga tcgtttttat aaattagcgc
      181 aacgatggct ttaagtgtca gatggcttcc ttcagcagac ggttgattgt ctgacgaaaa
      241 gacttcatgc acattgcgca gagtgatgat ggttgaaaca ataaaaaagg cgatcagtac
      301 cagtgtgaac atctgaaagc caaatccccg atcgccaccg ccgacataat taacaaatgg
      361 tagcgtcaca cctgccgtaa caaagcccgc cagactggca aaaaaacgcg gataaggaac
      421 cagttgttca cgctcacgtt tatcgagcgt gatggttgga accagcgacc agaagggaat
      481 atccataatg gtgtaagtca tgccccagag gatgtaggtc acgcaaacaa agacaatctg
      541 agtagtacct tcaaacagat gcgcactaaa gaggagaaat aagattacag agtttgccaa
      601 cgtaccgatc aggatccagg gtttgaactt accccatcgc gatcgcgtag catttacaat
      661 ccatcccata atcggatcgt taatagcatc ccagatcctc gccaccagaa acaaagtacc
      721 gaccaaaccc acagacagcc caacgacatc ggtgtagtaa tacatgaggt acatatacac
      781 aatgccgatc gcaaaatcct tcccgaacgc tccaaatcca taactgagtt ttgtagtcat
      841 tgaaatgctc atagggtatc gggtcgctgt taccaacgcc cgccttctgt tattggtatc
      901 gtcagaggtg tgcgccggaa taaatccccc ggcgcagtag cgtttagtcg cgttttaacg
      961 gtgcaaccag cctggcagcc agtcgccgtg ggcggcaatc aggtcgtcaa caagagcata
     1021 tatttcgtca atgcccagca cggcggcagt atgcgggtcc atcatcgcgg cgtggtaaac
     1081 acggtcgcga ttttccgtaa gaatagcttc ggtcagcagc gtctgtacat tgatgttggt
     1141 ttgcatcagg gcggccagat gcgaaggtag cgtaccgact ttagtcggct gaatgccatt
     1201 agcatcaacc agacaggcta cttccacgca acatccttgt ggcaggttat caatcaaacc
     1261 atcgttacgg acgttgccgt aaatcacact cggctcgcca gtccagatag cgttcatgat
     1321 tgtgctggca tattctcgtg acggtttaat atcaatccgg gaggcgtttt tatactcctc
     1381 cagctcttta tgccagttcg ccagctgctc gacgcagcgt ttcgggtact catccagcgg
     1441 tactttataa cgctcaatca aatcctcacg acctggctta ataaaccacg gcgtgtattc
     1501 agcaaaatgt tctgacgatt ccgtaacgaa gtagcccagc tttttgaaca tttcgtagcg
     1561 cacaatattc tggcagcgag tattaccatg aatatttggc ttcggtgcct gccctgcttc
     1621 ataagccgcc agcagttccg ggtagagatt cacgtaactg ccgtcggcgg ttttacgctc
     1681 cagttccagg taaaacgcca tatggttgat accagcgcaa cggtaacgca gcgtggacgg
     1741 atcgatattg aggtcacgcg ccagctcttc cgccgttccc tgcaccgaat ggcacagccc
     1801 tacctgtttg atatgcggat agcgggcata catcgcccag gtattcatcg ccattgggtt
     1861 aacgtagttg agcatggtgg catcggggca gacttccgtc atgtcctcgc aaatttgcca
     1921 cagatgcgga atggtacgta gcgcgcgcat aataccgccc ggccccaacg tatcggcaat
     1981 ggtttgttcc agaccatgcc gcttacagac ctcgaaatca gtcaccgtgc aaggttcata
     2041 accgccaatc tgaaatgcca ccacgacaaa atcggcatcc tgtaaggctt ctttctgttg
     2101 ggtgtggcag gtgattttgc cgctggcccc tgctgaatcc atcagcttac gcaccacaat
     2161 atgcgactct tccaggcggg tgggatcaat gtccatcagg gcaatatgcg ccgttttcag
     2221 cgcctcgcga tggaacacat caccaagaat atttttaacg aaaatcgtcg aaccagcgcc
     2281 gataaatgta attttgggtg cagtcatcat gcagatctcc tggcttgctt gaataacttc
     2341 atcatggcag gcgaatctgc agaaaacctc cggcttccag gttgagcatt cccataaact
     2401 cagatcctgc gtgaagcagc agtaaatctg agtttatggt aataagttac ctgaaaagca
     2461 gagttttcgg cctcatgatg ctcggacgct cccctcgcct ctctgggaag agggtgaaaa
     2521 ccgtgctccc actcgcagtc atcctccctc actcctgcca taattctgat attccaggaa
     2581 agagagccat ccatgaatac agatacgttt atgtgcagca gcgacgaaaa acagacccgc
     2641 agcccgctgt cgctgtattc ggaataccag cggatggaga ttgagtttcg cgcaccgcat
     2701 atcatgccca ccagccactg gcatggtcaa gtcgaagtga atgtgccttt cgatggcgat
     2761 gtggaatacc tgatcaacaa tgaaaaagtg aatatcaatc agggtaatat cacgctgttc
     2821 tgggcctgta caccgcacca actaacagat accggaacct gtcagagcat ggcgattttt
     2881 aatctgccga tgcatctgtt tctctcctgg ccgctggata aagacctgat taaccacgtc
     2941 actcacggca tggtgatcaa atcactggcc acacagcaac ttagcccgtt tgaagtgcgc
     3001 cgctggcagc aggaattaaa cagtccgaac gagcaaattc gccagctcgc cattgatgaa
     3061 attggcctga tgctcaagcg atttagcctc tctggctggg aaccgattct ggtcaataaa
     3121 acctcgcgca cacacaaaaa cagcgtctcg cgccatgcgc aattttatgt tagccagatg
     3181 ctgggcttta ttgccgaaaa ctatgatcag gcgctgacca tcaacgatgt ggctgagcac
     3241 gtcaaactta acgccaacta tgcaatgggg atattccagc gggtcatgca attgacgatg
     3301 aaacagtaca ttaccgcgat gcgcatcaac cacgttcgcg cgttactgag cgataccgat
     3361 aaaagtattc tcgatattgc cctgacggca ggctttcgtt caagtagtcg tttttacagc
     3421 acgttcggca aatatgtcgg catgtcgccg caacaatacc gcaaacttag ccaacagcgc
     3481 cgccagacgt ttcccggcta aagacgatat cagtatcagc caaaaaaata gtttagccgt
     3541 cgataataag cagtgaaaat aatttaaaat aatcacataa cgcattgttt ataaaaataa
     3601 atatttcaaa aatgtttgtt tttcacgcgc tttacagccc gaaaaggccg gaagatactt
     3661 gcccgcaacg aagattcctt cataaccggg taagcaatga tgaaagtatt aattgttgaa
     3721 agcgagtttc tccatcaaga cacctgggtc ggtaacgccg ttgagcgtct ggcagatgct
     3781 ttaagccagc aaaatgttac cgtgattaaa tccacctcct ttgatgatgg ttttgccatt
     3841 ctctcttcaa acgaagccat tgactgcctg atgttcagct atcaaatgga acatccggac
     3901 gaacatcaaa acgtcagaca attgatcggt aagcttcatg agcgccaaca aaacgtgccg
     3961 gtcttcctgt tgggcgatcg ggaaaaagcc ctcgccgcaa tggatcgcga cctgctggag
     4021 cttgtcgatg aattcgcctg gattctggaa gataccgccg actttatcgc cggacgcgct
     4081 gttgccgcga tgacccgcta ccgccagcag ctgttgccgc cactgttcag cgcgctgatg
     4141 aaatatagcg acatccatga atattcctgg gcagcgccag gccaccaggg cggcgttggt
     4201 tttaccaaaa cacccgccgg acgtttctac catgactact atggtgaaaa tctgttccgc
     4261 accgatatgg gcatcgaacg aacttccctc ggttctttgc ttgaccatac tggcgcattt
     4321 ggcgaaagcg aaaaatatgc cgcacgcgta tttggtgccg atcgctcctg gtcggtagtc
     4381 gtcggtactt ccggctctaa ccgcaccatc atgcaggctt gcatgaccga taacgatgtc
     4441 gtggtcgttg accgtaactg ccataaatcc atcgaacaag gcttgatgct gacaggcgcg
     4501 aaaccggtct acatggtgcc aagccgcaac cgctacggca ttatcgggcc aatctatccg
     4561 caggaaatgc aacctgaaac cttgcagaag aaaatcagtg aaagcccgct gactaaagac
     4621 aaagccgggc aaaaaccgtc ttactgcgtg gtgaccaact gcacctatga cggcgtgtgt
     4681 tataacgcta aagaagcgca ggatctgctg gaaaaaacct ccgatcgtct gcactttgac
     4741 gaagcctggt acggctatgc acgtttcaac ccgatctatg ccgatcacta tgccatgcgc
     4801 ggcgaacctg gcgatcacaa cggtcctacc gttttcgcca cccactccac ccacaaactg
     4861 ctgaatgcgc tttcacaggc ttcttatatt catgtacgtg aaggtcgtgg ggcgattaac
     4921 ttctcccgct tcaaccaggc ctacatgatg catgccacca cctccccgct gtatgccatc
     4981 tgcgcatcca acgacgtggc ggtgtcgatg atggacggca acagcggcct gtcactgacg
     5041 caggaagtga tcgacgaagc ggttgatttc cgtcaggcga tggcgcggct gtataaagag
     5101 ttcaccgctg acggtagctg gttcttcaaa ccgtggaaca aagaagtcgt caccgaccca
     5161 caaaccggca aaacctatga ctttgctgac gcaccaacca aactgctgac caccgttcag
     5221 gactgctggg taatgcatcc gggcgaaagc tggcacggct tcaaagatat tccggataac
     5281 tggagtatgc tcgacccgat taaagtcagc atccttgctc cgggaatggg tgaagatggt
     5341 gaactggaag aaaccggtgt tccggcggcg ctggtcactg cctggcttgg tcgccacggc
     5401 attgtgccta cccgcaccac tgacttccaa attatgttcc tgttctctat gggcgtaacc
     5461 cgtgggaaat ggggaactct ggttaacacc ctttgctcct tcaaacgcca ctatgacgcc
     5521 aacacaccgc tggcgcaggt gatgccggaa cttgttgaac aatatcctga cacttacgcg
     5581 aacatgggga ttcacgatct gggtgacacc atgtttgcct ggctgaaaga aaacaaccct
     5641 ggcgcacggt tgaacgaagc ctattccggc ctgtcggtgg cggaaatcac cccgcgtgaa
     5701 gcgtacaacg cgattgtcga caacaatgtc gaactggtat ccattgaaaa tctgccagga
     5761 cgcatcgcgg caaactcagt tatcccatat ccgccaggaa tcccgatgct gctgtctggt
     5821 gaaaacttcg gcgataaaaa cagtccgcaa gtaagttatt tacgctcgct gcaatcctgg
     5881 gaccaccatt tccctggatt tgaacacgaa actgaaggga ctgaaattat tgacggtatt
     5941 taccacgtta tgtgtgtgaa agcgtaacca ctattccgct gaaggcgtaa ttgtttaaat
     6001 aacattacgc cgcctggcct taggcctttt gagtatggca acgttttcat aaaaattgct
     6061 gcaaacaaaa atgtcatact ttttgcgcgg ccccaccccg cgcttttttt tgcctgttat
     6121 ttatcctgta aaaaaatatg tacatgagaa attactataa aaatttgtaa tattagtaaa
     6181 actcgttatt tttatgcatg tttatattca tcatacaatt atataaccat ttcccggtat
     6241 cgctttgctt tagcgagaac tggtctttta ttcgcactca ggagtacatg tatgaggatt
     6301 tgcagcgacc aaccttgtat tgttttattg actgaaaaag atgtctggat aagggtgaat
     6361 gggaaagaac ctattagcct taaagctaac catatggcgt tattaaattg tgaaaataat
     6421 attatcgacg tctcctctct taacaacact ttggttgctc atattagtca cgacatcatc
     6481 aaagattacc tccggtttct gaataaagat ctctcgcaaa taccagtatg gcaacgtagc
     6541 gctacgccca tactcaccct gccatgcctg acgccagacg tctttcgcgt tgccgcgcaa
     6601 cacagcatga tgcccgcaga aactgagtca gaaaaggaac gaacacgcgc attattattc
     6661 acggtgctat cccgttttct cgacagtaaa aaattccttt cattaatgat gtatatgtta
     6721 cgtaattgtg taagtgacag cgtttatcaa attattgaaa gcgatattca caaagactgg
     6781 aatcttagta tggtagccag ttgtttatgt cttagcccaa gtctgttaaa gaaaaagctg
     6841 aaaagcgaaa acaccagtta tagccaaata atcaccacct gccgcatgcg ttatgccgta
     6901 aatgaattaa tgatggacgg taaaaatatc tctcaggtat cacagtcctg cggctacaac
     6961 agtacgtcgt actttatttc tgtctttaaa gacttctacg gtatgacgcc gctgcattat
     7021 gttagtcagc acagagaacg aactgtcgcc tgatttttaa ccttaacgaa gagctatatt
     7081 aataacggca tcagcgataa cccggtcgat aataattcaa ctatcgaatg caggcgtatg
     7141 atatgacgta atttattgtc acgaagctcg ccttcgcagg agtttaatta tgtcttcgga
     7201 tgctgatgct cacaaagtgg gcttaatccc cgtcaccctg atggtgtcgg ggaatattat
     7261 ggggtcaggt gtttttctgt tacctgcaaa cctggcctcc actggcggga ttgccattta
     7321 tggatggttg gtgacgatta tcggtgcgct ggggctctcg atggtatacg ccaaaatgtc
     7381 gttcctcgat ccaagtcctg gtggttctta cgcttacgcc cgccgctgct ttggcccgtt
     7441 tctcggttat caaaccaacg tcctctactg gctggcctgc tggatcggca atatcgctat
     7501 ggtggtcatt ggcgtaggat atttaagtta cttcttcccg attctgaaag atccattggt
     7561 attaaccatc acctgcgtcg tggtgctgtg gatcttcgtc ctgctgaaca ttgtcggtcc
     7621 gaaaatgatc acccgtgtgc aggccgttgc caccgtgctg gcgctgatcc ccatcgtcgg
     7681 gattgccgta tttggctggt tctggttccg tggtgaaacc tatatggcgg catggaacgt
     7741 cagcggcctg ggcaccttcg gtgcgattca aagtaccctt aacgttacgc tgtggtcgtt
     7801 catcggtgtg gaaagtgcct ccgttgccgc aggtgtggtg aaaaacccga aacgcaatgt
     7861 ccctatcgcc accattggtg gggtattgat tgccgccgtt tgctatgtgc tctccaccac
     7921 cgcgattatg gggatgatcc ctaatgccgc actgcgcgtt tctgcctcgc cattcggtga
     7981 tgccgcacgg atggcgttgg gtgacaccgc tggggccatt gtttccttct gcgcagctgc
     8041 gggttgctta ggttcactgg gcggctggac gttgctggcg ggtcaaacgg cgaaagccgc
     8101 tgccgatgac ggactgttcc caccgatttt tgcccgtgta aataaagcgg gtacgccagt
     8161 ggcggggctg attatcgtcg gtattttgat gaccatcttc cagctcagta gcatttcgcc
     8221 aaacgcaacc aaagagttcg gtctggtatc ttccgtgtcg gtcatcttta cactggtgcc
     8281 atatctttac acctgtgcgg cgttactgct gctcggccac ggtcactttg gtaaagcacg
     8341 cccggcatat ctggctgtta ctaccattgc cttcctctac tgcatctggg ccgtggtggg
     8401 gtccggggcg aaagaggtta tgtggtcatt tgtcaccctg atggtcatca ccgccatgta
     8461 tgcgctgaat tacaaccggc tacataaaaa cccgtatccc ttagatgcac caataagcaa
     8521 agattaattc cccttaatcc agcaaacata aaagccaacc ttaagaactt aaggttggct
     8581 taattttgct ttgcgagcat atgcgcactt tgttcgatgg aaacaccgtg atgttgaagc
     8641 gcctactaaa aagcccctct ttgaatttac tcgcctggct attgttggcc gctttttata
     8701 tctctatctg cctgaatatt gcctttttta aacaggtgtt gcaggcgctg ccgctggatt
     8761 cgctgcataa cgtactggtt ttcttgtcga tgccggtcgt cgctttcagc gtgattaata
     8821 ttgtcctgac actaagctct ttcttatggc ttaatcgacc actggcctgc ctgtttattc
     8881 tggttggcgc ggctgcacaa tatttcataa tgacttacgg catcgtcatc gaccgctcga
     8941 tgattgccaa tattattgat accactccag cagaaagtta tgcgctgatg acaccgcaaa
     9001 tgttattaac gctgggattc agcggcgtgc ttgctgcgct gattgcctgc tggctaaaaa
     9061 tcaaacctgc cacctcgcgt ctgcgcagtg ttcttttccg tggagccaat attctggttt
     9121 ctgtactgct gattttgctg gtcgccgcac tgttttataa agactacgcc tcgttgttcc
     9181 gcaataacaa agagctggtg aaatccttaa gcccctctaa cagcattgtt gccagctggt
     9241 catggtactc ccatcagcga ctggcaaatc taccgctggt gcgaattggt gaagacgccc
     9301 accgcaaccc gttaatgcag aacgaaaaac gtaaaaattt gaccatcctg attgtcggcg
     9361 aaacctcgcg ggcggagaac ttctccctca acggctaccc gcgtgaaact aacccgcggc
     9421 tggcgaaaga taacgtggtc tatttcccta ataccgcatc ttgcggcacg gcaacggcag
     9481 tttcagtacc gtgcatgttc tcggatatgc cgcgtgagca ttacaaagaa gagctggcac
     9541 agcaccagga aggcgtgctg gatatcattc agcaagcggg catcaacgtg ctgtggaatg
     9601 acaacgatgg cggctgtaaa ggtgcctgcg accgcgtgcc tcaccagaac gtcaccgcgc
     9661 tgaatctgcc tggtcagtgc atcaacggcg aatgctatga cgaagtgctg ttccacgggc
     9721 tggaagagta catcaataac ctgcagggtg atggcgtgat tgtcttacac accatcggca
     9781 gccacggtcc gacctattac aaccgctatc cgccgcagtt caggaaattt accccaacct
     9841 gcgacaccaa tgagatccag acctgtacca aagagcaact ggtgaacact tacgacaaca
     9901 cgctggttta cgtcgactat attgttgata aagcgattaa tctgctgaaa gaacatcagg
     9961 ataaatttac caccagcctg gtttatcttt ctgaccacgg tgaatcgtta ggtgaaaatg
    10021 gcatctatct gcacggtctg ccttatgcca tcgccccgga tagccaaaaa caggtgccga
    10081 tgctgctgtg gctgtcggag gattatcaaa aacggtatca ggttgaccag aactgcctgc
    10141 aaaaacaggc gcaaacgcaa cactattcac aagacaattt attctccacg ctattgggat
    10201 taactggcgt tgagacgaag tattaccagg ctgcggatga tgttctgcaa acttgcagga
    10261 gagtgagtga atgaaaattc tgattgttga agacgatacg ctgttattgc agggactgat
    10321 tctggcggcg caaaccgaag gctacacgtg cgatggcgta acaaccgcgc ggatggcgga
    10381 acaaagcctt gaggccggtc attacagcct ggtggtactg gatttagggt tacccgacga
    10441 agatggactg cattttctcg cccgtatccg gcagaaaaaa tacaccctgc cggtactgat
    10501 cctcaccgct cgcgatacgc tgaccgacaa aatcgccggg ctggatgtcg gtgccgatga
    10561 ctatctggtg aagccttttg cgctggaaga gttacacgcc cgtatccgcg ccctgctacg
    10621 acgccataat aatcagggcg aaagtgagct gattgttggc aatctgacgc tgaatatggg
    10681 tcgccgtcag gtatggatgg gcggtgaaga gttgattctg acgcccaaag aatatgctct
    10741 gctgtcacgg ttaatgctca aagcaggcag tccggtacat cgggaaattc tctacaacga
    10801 catctataac tgggacaatg aaccctcgac caacaccctg gaagtgcata tccacaatct
    10861 gcgcgacaaa gtgggcaaag cccgtatccg caccgtgcgc ggctttggct atatgctggt
    10921 cgcgaatgag gaaaactaat tgaatctgat gcgttttctg cgccgaccaa tatcgctgcg
    10981 ccaacggctg atattgacca tcggggccat tttgttggtg tttgagctga tcagcgtctt
    11041 ctggctatgg catgaaagta ccgagcagat tcagctgttt gagcaggcgc tacgcgacaa
    11101 tcgcaacaac gatcgccaca tcatgcgtga gatccgcgag gcggtcgcca gcctgattgt
    11161 ccccggcgtc tttatggtca gcctgacgct atttatctgc tatcaggcgg tacgccgcat
    11221 cacccgcccg ctggcggagc tgcaaaaaga gctggaagcg cgcaccgccg acaacttaac
    11281 gcccattgcc attcacagcg ccaccctcga aatcgaagcg gtggtttcgg cgttgaacga
    11341 tctggtcagt cgcctgacga gcacgctgga taacgaaagg ttatttaccg ctgacgtcgc
    11401 gcacgaactg cgaacgccac tggcgggggt gcgtttgcat ctggaactgc tggcgaaaac
    11461 gcatcacatt gatgtagcac cgttagtggc acggcttgat cagatgatgg agagcgtctc
    11521 ccagctgctg caactggcgc gtgccggaca gtcattttct tccggtaatt atcaacatgt
    11581 aaaactgctg gaagatgtca ttctcccctc gtatgacgaa ctcagtacca tgctcgacca
    11641 gcgacagcaa accctgctat tgccagagag cgccgccgac atcaccgttc agggtgatgc
    11701 aaccctgctg cggatgttat tgcgaaacct ggtagaaaac gcccatcgtt acagcccaca
    11761 aggcagcaac attatgatta agctgcaaga agatggcgga gcggtcatgg ccgttgaaga
    11821 tgaaggaccg ggtattgatg agagtaaatg cggggagttg agtaaagcgt ttgtacgtat
    11881 ggacagccgt tatggcggga ttggtctggg gttaagtatt gtcagccgca ttacacagtt
    11941 gcatcacggg cagtttttcc tgcaaaaccg gcaagagact tccggcacgc gggcctgggt
    12001 acggctgaag aaagatcagt acgtagcaaa ccagatataa agaaagctgc tgaccaccag
    12061 cacactgaac acggtagtta aactttcata aacacggttt ttcattgcac tctcctccgt
    12121 taacctggag gagagtatgc gcgtcagaga ttaagtgaac cttaagagtt caattggcct
    12181 gataagacag cgtcacatca ggccatccgt ttcagcttat tcatcaattc gcggatgttg
    12241 ctgcaccagg cgggtacgtt tcgcctgcaa atcggcaatc tcgtggtcaa tatcatcgat
    12301 tttctgctcg atattatcgt aatgctcgac gagaatttcc ttcgcttcct gtatatctga
    12361 cgccgccggt gtcgcacctt tcaacggacg atttgccgtc tctttcatgg ttacgccggt
    12421 gattaaacca atcaccgcca ctaccatcag gtaataggca ggcatcatca gattctgcga
    12481 gctttcgacc agccaggccg ccagtgttgg cgtcagaccg gcaaccagca ccgaaatatt
    12541 aaatgccgcc gccagcgcgc tgtaacggat atgcgtcggg aacatcgctg gcaaggtaga
    12601 agccataacg cccgtaaagc agttaaggat caccgccagc atcagtaacc cggcaaaaat
    12661 caggccgatg acgttactgt taatcagaat aaacgccggg atcgccaaca caaacagggc
    12721 aacactacca agtagcacaa acggacgacg gccaaaacgg tcactcagca agcccatcac
    12781 cggctggaca aacagcatac cgatcataat ggcgataata atcagcaccc cgtggtcttc
    12841 ggagtaatgc aggttatgcg acaaataact cggcatatag gtcagcaaca tgtagtaagt
    12901 cacgttggtg gcaattacca gaccaataca tgtcaacagg ctgcgccagt atttagtggc
    12961 aatctcttta aacgagactt tcgggccatc ctgcaaacct tcgcggtcgc cctgttccag
    13021 tttatcaaca tgctgctgga acgccggagt ttcttccagc gcatggcgca ggtaaagccc
    13081 gataatccct aacggcagag caataaagaa cggaatacgc cagccccagt cgaggaagtt
    13141 cgcttcgccg acaatggtcg aaattaacac caccacgccc gcacccagca caaacccggc
    13201 aatagaaccg aagtccagcc agctgcccat aaagccacgt ttacggtcag gggagtattc
    13261 cgcaacaaat atcgacgccc cggtatattc accgccgacc gagaaacctt gtgccatctt
    13321 acagatcaac agcagaatcg gtgcccaaat accaatcgtg tcgtaggacg gtattaagcc
    13381 aatacagaac gtactgatcg acataatcac aatagtgata gcgaggatct tctggcgacc
    13441 atatttatcg cccaacatac caaagaagag cccgccaagc ggtcgaatca gaaagggaac
    13501 ggagaaagtg gcaagtgcag caaccatctg cacgctgggg tcagcccccg ggaaaaaaac
    13561 tttacctaat gcgtaagcaa caaaaccata aacaccaaaa tcgaaccatt ccattgcatt
    13621 acccagtgat gctgcggtaa tggctttacg cagtttaccg tcatcaataa tggtgacatc
    13681 acgaagggta atcggtttta cttttttcct tttcagcata gctttcctcg cagagttggc
    13741 ccatttccgc aaacgccaga aaaatcagcg cgacggccgc aaaaggggag cgggaattgc
    13801 aggatgcaat ctctgtaaca agcgtagcgg gtttgcttac accctccggt taaggaatgt
    13861 acaatcaaac cagttgacgc ctgaattggg cagttaatga cctttttacc ctaccagtgt
    13921 ttaaatttgt gatcaacttc acacatggat ttcgtttagg actcattgat gtaactgatt
    13981 atttacgcca aatttacaga gaagatttcc tcgaccattg cccccaccaa acgctgcgct
    14041 gaacagagtc gtggcatccc cagcgcgact gtttgccagc gttgggtcac tgaccagcaa
    14101 acgggctgtt tatctgccgt ggaccacttt cccagccaac ttaaaaaatc agcatgctcc
    14161 cctcccgaag aggcaaacca ctgatggtaa tagtgacgcg tggtcggtgc cgcattgata
    14221 ctctctgcca gatgttgtgt cgctttctcg cgcaaatcac ccagtaacaa caccaccgct
    14281 tgcgggcttc ccagacggca ggcatcgcca aatgcacccc agcgcaactc ctccagaacg
    14341 ataaaacagc gcccaggcaa cgaccaggcg ttatacgccc cagcacgcca gcgggtaaaa
    14401 atttgctcgc tctgatcacc tgcctgcacc agtaggcctc gttgcgttaa cgccttttcc
    14461 ttccagcgac aacgggctga aaaccactca tacaaggcgg caatttgctg ggggagctcc
    14521 gggaaagcag agtgctggcg gtgatccatt ttttgcagca gcgccagatg ctgcgcgatc
    14581 tgcgtcagcg ccagatgacc gggatccagc atcgacgcca gtttttccac cattgtcagt
    14641 cgcaggctaa gcggcgcatt taacatgccg ccgttcgtcc atgtctgaag ctgcacacgc
    14701 gaaagaagtt cctgcatcag tcgttcacga aactgctgct gatgggcttg tggaaggcgg
    14761 gcatcatcgc cctgcgccag atccactaaa aagcggggat aaaccgactc cagcacgcga
    14821 ccggggccgt ccagtaacgt cttggtcaat atcgttctgc cgtgaaaagt gtttgaatat
    14881 catcgcgtaa cagctgggcg tcggtgtaaa tccagccgtg agtcatcaca gtctgctgca
    14941 attgctgctg catcagcctg accaccgatt cattttgttg acgcagagcc aggctttcgc
    15001 gtaaacgcgt ctgtaattcc gtcaaacatg aagcgaactc agcgcaaaaa gtattcatgc
    15061 ctgccgtaac agattcatcg acctgctctg ccagaacttt agccatttgt tggcaataaa
    15121 gatcgacttc tgcgcttaaa gctcgttgca acacactgta atcaaccgtc tctgtcgggg
    15181 atttctcatt tccccgtccc cagtcgggct gattcaacca gcgcgaaaaa gtctcacgca
    15241 caacgcctaa acgcgtgctc tgctcgtccg ttgcatcctg gcgcgaaatg acttcactga
    15301 acagctggcg agtgttgacg tggggaacta ctccgtgaaa aacaggaaaa tgaaacccag
    15361 gacgaaaccc tgactcgctc aattccattt tgacttgttg ctcaatgggc cgaataacat
    15421 cggttaacac ccggcaaagg gtggattcaa gctcggcaaa acgcagcgta aagtcgcgac
    15481 tgatggtgtt ctgcgccgtc tgtaacagtg tctcacagca ggtacgaatc tcgcttaacg
    15541 gctccgaatc atcctgaaac aaggcggcta actgcgcatt cagcgcatct tgctgttgac
    15601 gcagaaagtg gttggcggag gtcagggcca gctcgatttc atgtttaatc tcgccgctca
    15661 cctgcgcctg attgagttgc aatagctgca aactttcttc gacctgatgg atattttgcc
    15721 gcaattgttc acaagcgacg tttaacccgt gcgcacgaaa atccaggtat tcccgcgcct
    15781 gctgcgcgta attcaacagt ttatgcgcag cagatcgcag agcatacaac gaggcgttag
    15841 cgtaagcggc atgaagcaac gcctgaattg gctgggcgaa cagcgaatct tcccacaact
    15901 gatcggcagc atgacgaata tgttcgaggt ccgccagatc ggcatgacgc cagcgcctgc
    15961 cgagcgcggc atgggcaaaa tcttccaccc agcgttgttg ctctggcgct ggtaacttac
    16021 cgttgttggc taactcatgg cgcgctcgat ttgccaagta gccccacatc gacgacaccg
    16081 gaaatatctg ctgtggcgta atacagcctt tcatcagcgt cccggaaatc agtgcccgca
    16141 cctggtcggc gtcgtcactg ttacgatcct gttgatcgaa cttattgacc agcacataca
    16201 gtggcaccga ttgccccacc gccaaaatcg cctcacggac ctcttcatcg gagatcgatt
    16261 tcagttgcgt ataatccagc accgccagta ccgccgaggc gcgtgccagc tgctggttaa
    16321 gcattttttg cagatgcggt tgcccggctt catttggccc gggggtatcc agtaacgtca
    16381 actgaccggg ataactctcc agccccgcca gatggacaaa ctccacttca atcacgggaa
    16441 tatgctcaat ggcggcgtaa gcagaaaaag gaaaatcgac gtccagcgcc ttcgccagtc
    16501 gcactaaatc attcaaactt ttcagacaat gaaaaatagg ctgggcaccc agataatatt
    16561 tttcgaaagc gacgccattt tcgatccgct gcataagcgc acgcatatct ttatctattt
    16621 ccagcacatc ggtcagatgc ttaatatcgc aatcacgcag gcgctgttgt aattgttgaa
    16681 ttaaacaatc gattggcgcg acatgtgaaa aatgcagtac cggttccttt tgcccgggcg
    16741 tatggcgaat aagcgtcggc agcgcagtca ttgggcgatt acgattaggc agaacctccg
    16801 taccaacaat ggcattaatg gtggttgatt tccctgcttt catagtaccg acaatcgcca
    16861 gcaccatttc cagtcgggaa attttactca actcattatt cagcatcgcg tgacgttcgg
    16921 cgatattagg ctgactccag ggtaaagcca gttgtggcgc gtcgtctccg ggtacagaga
    16981 gaggcatttt ttccagtaac tgcaactgtt ggcgagaaag ctgtaacagg cgttcagcct
    17041 cctgacttaa ctcatacagg gtctgtgtgt acatagaaaa ttcttcctta aagcaaattt
    17101 tgttactttt atttagccag attgtttttg agttctgttt ttggctttta taattactgc
    17161 aagaaataat tttatattta gtgtgttgtt ttttatcaga ataaataacg tcttctgatg
    17221 cgtttaaaac gtcagaaaga taaaaatatc atgtgaataa aaaaaagaac aagtagagca
    17281 ttaacattat cttaaataat aaatagaggc aaaaagatta ttttcttttt gcgtttcctt
    17341 tcaaatgaaa acgatcgtcg tctaaaatca gcagtacccc cgacaaactc agggattttg
    17401 tgtataattg cggccttttt cggcaatctg ccgttttttg gcgcttttgc cctgctgact
    17461 tttgaggaaa tccacatgtc attaccacac tgcccaaaat gcaactccga atacacttac
    17521 gaagataacg gcatgtacat ctgcccggaa tgtgcctacg aatggaacga cgcagaacct
    17581 gcacaggaaa gcgacgagct gatcgttaaa gatgctaacg gcaatctgct ggctgacggc
    17641 gacagcgtta ccatcattaa agatctgaag gtgaaaggta gctcttcgat gctgaaaatt
    17701 ggcaccaaag tgaaaaacat ccgcctggtt gaaggcgacc ataacatcga ttgcaaaatc
    17761 gacggttttg gtccgatgaa actgaaatct gagtttgtga aaaagaactg attgtattgt
    17821 gatcggtaag ccggataagg cgctcgcgcc gcatccggca acgatgccga ctgcctgatg
    17881 cgacgcttgc gcgtcttatc aggcagcctc tcatctgtat aaatttcgaa ctacacttaa
    17941 ttggcttctc ttaactgagg tcaccatcat gccgttaagt ccctacctct cttttgccgg
    18001 taactgtgcc gacgcaattg cttattatca acgtacgttg ggcgctgaac tgctctataa
    18061 aatcagcttc ggcgaaatgc caaaatcagc gcaggacagc gccgagaact gcccttccgg
    18121 aatgcaattt cccgataccg ccatcgccca cgccaatgtg cgcattgccg gaagcgacat
    18181 catgatgagc gatgccatgc cgtcaggaaa agccagctac tccggcttta cgctggtgct
    18241 cgattcgcaa caggtcgaag aaggaaaacg ctggtttgac aatcttgccg ctaacggaaa
    18301 aatcgaaatg gcctggcagg aaactttcta ggcgcatggc tttggcaaag tcaccgataa
    18361 atttggcgta ccgtggatga ttaatgtcgt caaacaacaa ccaacgcaat aacccgccgg
    18421 gaggcccgcc ctcccgcact gtcatcgaat tcccgttaac tcttcatctg ttagtcactt
    18481 ttaattaacc aaatcgtcac aataatccgc cacgatggag ccactttttt agggaggctg
    18541 catcatgcaa acgattatcc gtgtcgagaa gctcgccaaa accttcaatc agcatcaggc
    18601 gctgcatgcg gttgatctga acattcatca cggtgaaatg gtggctctgc ttgggccgtc
    18661 gggttccggc aaatccaccc ttttacgtca cttaagcggt ttgattaccg gcgataaatc
    18721 cgccggcagc catatcgagc tgctgggccg cacagtccag cgcgaaggcc gtctggcgcg
    18781 cgatatccgc aaaagccgcg ccaacaccgg ctacatcttc caacaattca acctggtgaa
    18841 ccgcctgagc gtactggaga acgtgctgat tggcgcactc ggcagcacgc cgttctggcg
    18901 cacctgtttt agctggttta cccgcgagca gaaacaacgc gcgttacagg cgctgacccg
    18961 cgttggcatg gtgcattttg cccatcaacg cgtttccacc ctctccggcg gacagcagca
    19021 gcgtgtggcg attgcccgcg cgctgatgca gcaggcgaag gtgattctgg ccgatgaacc
    19081 catcgcctcg ctggacccgg aatccgccgg catcgtgatg gacaccctgc gcgacatcaa
    19141 tcagaacgac ggcatcaccg tggtcgtcac gctgcatcag gtggattacg ccctgcgcta
    19201 ctgcgaacgc atcgtcgccc tgcgccaggg gcacgttttc tacgacggca gcagccaaca
    19261 gtttgataac gaacgttttg accatctcta ccgcagcatt aatcgcatcg aagagaacgc
    19321 gaaagctgcc tgacatcccc atcattgagg aaaacgaatg aacgctaaga taattgcctc
    19381 gctggccttc accagcatgt tcagcctcag caccctgtta agcccggcac acgccgaaga
    19441 gcaggaaaag gcgctgaatt tcggcattat ttcaacggaa tcacagcaaa acctgaaacc
    19501 gcaatggacg ccattcttac aggatatgga gaagaagctg ggcgtgaagg tgaacgcctt
    19561 ctttgcccca gactacgcag gcattatcca gggaatgcgc ttcaataaag tggatatcgc
    19621 ctggtacggc aacctgtcgg caatggaagc ggtggatcgc gccaacggcc aggtcttcgc
    19681 ccagacggtc gcggcggatg gatcgccagg ttactggagc gtgttgatcg tcaacaaaga
    19741 tagtccgatc aacaacctga acgatctgct ggcgaagcgg aaagatctca ccttcggcaa
    19801 tggcgatcct aactccacct ctggcttcct cgtccccggt tactacgtct ttgccaaaaa
    19861 caatatctcc gccagcgact ttaagcgcac cgtcaacgcc gggcatgaaa ccaacgcgct
    19921 ggccgtcgcc aacaagcagg tggatgttgc caccaacaac accgaaaacc tcgacaagct
    19981 gaaaacctcc gcgccagaga agctgaaaga actgaaggtg atctggaagt cgccgctgat
    20041 cccaggcgat ccgatcgtct ggcgcaagaa tctctccgaa accaccaaag acaagatcta
    20101 cgacttcttt atgaactacg gaaaaacgcc ggaagagaaa gcggtgctgg aacgcctggg
    20161 ctgggcaccg tttcgcgcct ccagcgacct gcaactggtg ccgattcgcc agctcgcact
    20221 gtttaaagag attcagggcg taaaaagcaa taacggactg aatgagcagg acaagctggc
    20281 gaaaaccacc gagattcagg cgcagctgga tgacctggac cgcctgaaca acgcgttaag
    20341 cgcgatgagt tcggtgagta aagcggtgca gtaatgtgct cgatcggtcc cctctccctt
    20401 gagggagagg gttagggtga gggggaaatg cgcacgctga tgccctcacc ccggccctct
    20461 cccacaggga gagggagaaa acattaaacg gagccaaaca tgcaaaccat caccatcgcc
    20521 ccacccaagc gcagctggtt ctcgcttctg agctgggcca ttgttctcgc cgtgttggtc
    20581 gtctcgtggc agggcgcgga aatggccccg cttacgctga tcaaagacgg cggcaacatg
    20641 gcgaccttcg ccgccgactt cttcccgccc gatttcagcc agtggcagga ttacctcacc
    20701 gaaatggccg tcacgctgca aatcgccgtc tggggcaccg cgctggcagt ggttctctcc
    20761 atccccttcg gcctgatgag cgccgaaaac ctggtgccgt ggtgggttta ccagcccgtt
    20821 cgccgcctga tggacgcctg ccgcgccatt aacgaaatgg tcttcgccat gctgttcgtg
    20881 gtcgccgtcg gtctcggacc gttcgctggc gtgctggcgc tatttattca caccaccggc
    20941 gtgctctcga aactgctttc cgaagcggtg gaagcgattg aacccggccc ggtggaaggc
    21001 attcgcgcca ccggcgccaa caagctggaa gagatccttt acggcgtgct gccacaggta
    21061 atgccgctgc tgatctccta ctccctctat cgcttcgaat ccaacgtccg ctcggcgacc
    21121 gtcgtcggca tggtcggtgc aggcgggatc ggcgtcaccc tgtgggaagc gattcgcggt
    21181 ttccagttcc aacaaacctg cgccctgatg gtgcttatca tcgtcacggt cagcctgctg
    21241 gatttcctct ctcaacggtt gcgtaagcac tttatctgat aagtgaggca ttgatatcta
    21301 tgcacttgtc tacacatccg accagctacc caacacgcta tcaggagata gccgcaaaac
    21361 ttgagcagga gcttcgtcaa cactatcgct gcggcgacta tcttcccgcc gagcagcaac
    21421 tggcggcgcg ctttgaggta aaccgccaca ccctgcgccg cgccatcgac caactggtgg
    21481 aaaaaggctg ggtacagcgc cgtcagggca tcggcgtgct ggtgctgatg cgcccgttcg
    21541 attacccgct caacgcccag gcgcgtttta gccagaacct gctggaccag ggcagccatc
    21601 ccaccagcga aaaactgctg tcggtattgc gccccgcgtc cggtcacgtt gctgacgcgc
    21661 tggggattac cgagggtgag aacgttatcc atttgcgcac cctgcgtcgc gtcaacggcg
    21721 tcgcgctctg tttaatcgac cactactttg cggacctcac cctctggccg acactgcaac
    21781 gtttcgacag cggttcgctg cacgattttc tgcgcgagca aaccggaatt gcgctgcgcc
    21841 gcagccagac gcggatcagc gcccgccgcg cccaggccaa agagtgccag cgccttgaaa
    21901 tcccgaatat gtcgccgctg ctgtgcgtgc gcacccttaa ccaccgtgac ggtgaaagca
    21961 gcccggcgga gtactccgtc agcctgacgc gcgccgacat gattgaattc actatggagc
    22021 actgaatgca cgcagatacc gcgacccgcc agcactggat gtccgtactg gcgcacagcc
    22081 aaccggctga actggcagcc cgcctgaacg cgctgaacat caccgccgac tatgaagtga
    22141 tccgcactgc cgaaactggc ctggtgcaga ttcaggcgcg aatgggcggt accggcgaac
    22201 gcttttttgc cggcgacgcc acgctgaccc gtgccgccgt acgcctgact gacggcaccc
    22261 tcggctacag ctgggtgctg gggcgtgata aacagcacgc cgaacgctgc gcgctgattg
    22321 atgcgctgat gcagcaatct cgccactttc aaaacttatc agaaaccctt attgccccgc
    22381 tggacgctga ccgtatggca cgcattgccg cacgccaggc cgaagtgaac gccagccggg
    22441 tcgacttctt tacgatggtt cgcggagaca acgcatgacc ctggaaaccg cttttatgct
    22501 tcccgtgcag gatgcccagc acagttttcg tcgcctgtta aaggccatga gcgagccggg
    22561 cgtgattgtc gccctgtatc agctcaaacg cggctggcag ccgctgaata tcgccaccac
    22621 cagcgtgctg ctgacgctgg ccgataacga cacgccggtg tggcttgcca cgccattaag
    22681 taacgatatc gtcaatcaga gcctgcgttt tcataccaac gcgccgctgg tcagccagcc
    22741 ggaacaggcg accttcgcgg tgacggatga ggcgatttcc agcgaacagc tcaacgccct
    22801 ttccaccgac accgccgttg cgccggaagc gggcgcgacg ctgattttac aggtcgccag
    22861 cctgagcggc ggacgcatgt tgcgtctcac cggcgcgggc attgccgaag aacgaatgat
    22921 cgctccgcag ctgccagagt gcattctgca cgaactcacc gagcgcccgc acccgttccc
    22981 gctcggcatc gacctaatcc tgacctgcgg cgagcgcctg ctggctatcc cgcgaaccac
    23041 tcatgtggag gtgtgctgat gtacgttgcc gtgaaagggg gcgaaaaggc gatcgacgcc
    23101 gcccacgccc tgcaagagag ccgacgccgg ggcgataccg atttgcccga actgagcgtc
    23161 gcccagattg aacagcagct taacctcgcg gtggatcgcg tgatgacaga aggcggcatt
    23221 gccgaccgcg aactggcggc gctggcgctg aaacaggcca gcggcgataa cgtcgaagcg
    23281 attttcctgc tacgcgccta ccgcaccacg ctgacgaaac tggcggtaag cgagccgctc
    23341 gacaccacgg agatgcgtct cgaacggcgt atttcagcgg tttataaaga tattcccggc
    23401 ggccagctgc ttggcccaac ctacgactac acccatcgcc tgctcgattt cactctgctg
    23461 gcaaacggcg aagcgccgac gctgaccacc gccgacagcg aacaacagcc gtcgccgcac
    23521 gttttcagcc tgctggcgcg tcaggggctg gcgaagtttg aagaggatag cggagcacag
    23581 ccggatgaca tcacccgcac gccgccggtt tacccctgtt cacgttcttc ccgtttgcag
    23641 cagttaatgc gcggcgacga aggctatttg ctggcgctgg cttactccac ccagcgcggt
    23701 tacgggcgca accacccgtt cgcgggcgag atccgcagtg gttacatcga cgtgtcgatt
    23761 gtgccggaag agctgggatt tgcggtaaac gtcggcgaac tgctgatgac cgagtgtgaa
    23821 atggtcaacg gttttatcga cccgccggat gagccgccgc acttcacgcg cggctacggg
    23881 ctggtattcg gcatgagcga gcgcaaagcg atggcaatgg cgctggttga ccgtgcgttg
    23941 caagccccgg aatacggcga gcacgcgaca ggcccggcgc aggatgaaga gttcgtgctg
    24001 gcgcatgccg acaacgtcga agccgcaggc tttgtctcgc acctcaaact cccgcactac
    24061 gtcgatttcc aggccgaact ggagctactc aaacgtctgc aacaggagca gaaccatggc
    24121 taatctgagc ggctacaact ttgcctacct cgacgagcag accaaacgca tgatccgccg
    24181 cgccatctta aaagcggtgg cgatccccgg ttatcaggta ccgtttggcg ggcgcgagat
    24241 gccgatgccg tacggctggg gaactggtgg tatccagctc accgccagcg tgattggcga
    24301 aagcgacgtg ttgaaggtga ttgaccaggg tgcggatgac accaccaacg ccgtgtcgat
    24361 tcgcaacttc tttaagcgcg tgaccggggt aaacaccact gaatgtacgg acgatgcgac
    24421 gcttatccag acgcgtcacc gcatccccga aacgccgctg accgaagatc agatcattat
    24481 cttccaggtg ccaatcccgg aaccgctgcg ctttatcgag ccgcgcgaaa cggagacccg
    24541 cactatgcac gcgctggagg agtacggcgt gatgcaggtg aaactgtatg aagatatcgc
    24601 ccgcttcggt catatcgcca ccacctatgc ttatccggtg aaggtgaacg ggcgctacgt
    24661 gatggacccg tcgccgatcc cgaaattcga taacccgaaa atggacatga tgcccgccct
    24721 gcagctgttc ggcgcagggc gcgagaagcg catctatgcg gtgccgccgt ttacccgcgt
    24781 ggaaagtctc gatttcgacg atcacccgtt caccgttcag cagtgggatg agccatgcgc
    24841 catctgcgga tcgacccaca gctatcttga tgaagtggtg ctggatgacg ccggaaaccg
    24901 catgtttgtc tgctccgata ccgattattg ccgccaacag agcgaggcga aaagccaatg
    24961 aatcaaccgt tactttcggt caataacctg acccaccttt acgcgccggg caaaggcttt
    25021 agcgatgtct cttttgattt atggccgggg gaagtgctgg gcattgtcgg ggaatccggc
    25081 tccgggaaga ccacgctgct gaagtcgatc tccgcgcgtc tgacgccgca gcagggggag
    25141 attcactatg aaaaccgttc cctctatgca atgagcgagg ccgaccgccg tcgcctgctg
    25201 cgcaccgaat ggggcgtggt gcatcagcat ccgctcgacg gcctgcgccg ccaggtgtcg
    25261 gcaggcggca atatcggcga gcggctgatg gcgaccgggg cgcgacatta cggcgatatt
    25321 cgcgctaccg cgcagaagtg gctggaagag gtggagattc ccgccaaccg tatcgacgac
    25381 ctgccgacca ccttttccgg cggtatgcag cagcgtttgc agattgcccg caacctggtg
    25441 acgcatccga agctggtgtt tatggatgaa ccgaccggcg ggctggatgt gtcggtgcag
    25501 gcccgcctgc tcgacctgct gcgcggactg gtggtggagc tgaacctcgc ggtagtgatt
    25561 gtcacccacg atttaggcgt cgcccgcctg ctggcggacc gtttgctggt gatgaagcag
    25621 gggcaagtgg tggagagtgg gttaaccgac cgcgtgctcg acgacccgca tcatccgtat
    25681 acacagctgc tggtgtcatc ggttttgcag aattgagccg gtgccggatg cggcgtaaac
    25741 gccttatccg gcctacaaat gcgctccccg taggtcggat aagacgcgtc agcgtcgcat
    25801 ccgacacccg aaccacgagg cgaaaaatga ttaacgtaca aaacgtcagt aaaaccttca
    25861 tcctgcacca gcaaaacggc gtgcgcctgc ccgtcctcaa ccgcgcctcg ctcatcgtca
    25921 acgcgggcga atgcgtggtg ctccacggcc attccggcag cggaaaatca accctgctac
    25981 gctcgctgta cgccaactat ctgcccgacg aagggcaaat ccagatcaaa cacggtgacg
    26041 aatgggtcga tctggtcacc gcgccagcgc gcaaagtggt ggagatccgc aaaaccaccg
    26101 ttggctgggt gagccagttt ctgcgcgtca tcccgcgtat ttcggcgctg gaagtggtga
    26161 tgcagccgct actcgatacc ggcgtaccgc gtgaagcctg cgccgctaaa gccgcgcgtc
    26221 tgctcacccg cctgaacgtg ccggaacgcc tgtggcatct ggcgccatcg accttctccg
    26281 gcggcgaaca gcagcgcgtc aacatcgccc gcggctttat cgtcgactac cccattctgc
    26341 tgctcgacga acctaccgcc tcgctggacg ccaaaaacag cgccgccgta gtcgagctga
    26401 ttcacgaagc caaagcccgt ggcgcggcca tcgtcggcat cttccacgac gaagccgtac
    26461 gtaatgacgt tgccgaccgc ctgcacccaa tggaagcctc ttcatgatta tcaataacgt
    26521 caaattggtg ctggaaaacg aagtggtaca cggttcactg gaggtgcagg acggcgaaat
    26581 ccgcgccttt gccgaaagcc agagccgcct gccggaggcg atggacggcg aaggcggctg
    26641 gctgctgccg gggctgattg agctgcatac cgataatctg gataaattct tcaccccgcg
    26701 cccgaaagtt gactggcctg cccactcggc gatgagcagc cacgacgcgc tgatggtggc
    26761 gagcggcatc accaccgtac tggatgccgt ggcaattggc gacgtgcgcg acggcggcga
    26821 tcggctggtg aatctggaga agatgatcaa cgccatcgaa gagacgcaga aacgcggcgt
    26881 caaccgcgcc gagcaccgtc tgcatctgcg ctgcgaactg ccgcatcaca ccacgctgcc
    26941 gctgtttgaa aaactggtgc agcgcgagcc ggtgacgctg gtgtcgctga tggaccactc
    27001 gccgggccag cgccagttcg ccaatcgcga gaagtatcgc gaatattatc agggcaaata
    27061 ctccctcact gatgcgcaga tgcagcagta cgaagaagag caactggcgc tcgccgcacg
    27121 ctggtcgcag ccgaatcgcg aatccatcgc cgccctgtgc cgcgcgcgaa aaattgcgct
    27181 tgccagccac gatgacgcca cccacgccca cgttgctgaa tctcaccagc ttggcagcgt
    27241 gatcgccgaa tttcccacca cgttcgaagc ggcggaagcc tcgcgcaagc atggcatgaa
    27301 cgtgctgatg ggcgcgccga atattgtgcg cggcggctcg cactccggca acgtggcagc
    27361 cagtgaactg gcgcagcttg gcctgctgga tatcctctct tccgactact accccgccag
    27421 cctgctcgat gcggcatttc gcgtcgccga tgacgagagc aaccgcttta cgctgccgca
    27481 ggcggtgaag ctggtgacta aaaatccagc gcaggcgctt aatctccagg atcgcggggt
    27541 gattggcgag ggcaaacgcg cggacttggt gctggcgcat cgcaagggca atcacattca
    27601 tatcgaccac gtctggcgtc agggtaaaag ggtgttctga tgatgggaaa actgatttgg
    27661 ttaatggggc cgtccggctc cgggaaagat agcctgctgg cggaactccg cctgcgggaa
    27721 caaactcagc tactggtggc gcatcgctac atcacgcgcg acgccagcgc cggaagcgaa
    27781 aaccatatcg ctctgagcga gcaggagttt tttacccgcg cggggcagaa cctactggcc
    27841 ttaagctggc acgctaacgg tctgtattat ggcgtcggcg tcgagatcga cctctggctg
    27901 cacgccggat tcgacgtgct ggtcaacggc tcacgcgccc atctgccgca ggcgcgggcg
    27961 cgctatcaat cggcgctgct gcccgtctgt ttacaggttt cgccggagat cctccgccag
    28021 cgcctggaaa accgtggccg tgaaaacgcc agtgaaatta acgcccgcct ggcgcgcgcc
    28081 gcccgctata ctccacagga ttgccatacg ctcaacaatg acggcagcct gcgccagtcg
    28141 gtcgacacgc tgctgacgct gattcatcag aaggagaaac accatgcctg cttgtgagct
    28201 tcgcccggcc acgcagtatg ataccgacgc ggtttacgcg ctgatttgtg agctaaaaca
    28261 ggcggagttt gaccaccacg catttcgcgt gggctttagc gccaatctgc gcgacccgaa
    28321 catgcgctat catctggcgc tgctggatgg cgaggttgtc ggcatgatcg gcctgcattt
    28381 gcagtttcat ctgcatcatg tcaactggat cggcgaaatt caggaactgg tagtgatgcc
    28441 gcaggcgcgc ggcctgaacg tcggtagcaa gttgctggcg tgggcagaag aagaagcccg
    28501 ccaggccggg gccgaaatga ccgagctttc gaccaacgtg aagcgccacg acgcgcaccg
    28561 tttctatctg cgagaaggct acgagcagag tcacttccgc ttcaccaagg cgctgtaaca
    28621 tgagtctgac cctcacgctc accggcaccg gcggcgcaca gggcgttccg gcatggggct
    28681 gcgagtgcgc ggcctgcgac cgagcgcggc gctcgccgca gtatcgccgc caaccgtgca
    28741 gcggcgtagt gaagtttaac gaggcgatca ccctgatcga cgccgggctg cacgatctca
    28801 ccgatcgctg gtctcccgga tcgttccagc agtttttgct gacgcattat catatggatc
    28861 acgtccaggg gctgtttccg ctgcgctggg gcgtgggcga tccgatcccg atctacggcc
    28921 cgccggatga acagggctgc gacgatctgt ttaaacatcc cgggctgctt gatttcagcc
    28981 acacggtgga accgtttgtg gtgtttgatt tgcaggggtt gcaggtcacg ccgctgccgc
    29041 ttaaccactc aaaactcact ttcggctatt tactggaaac ggcgcacagc cgggtggcgt
    29101 ggctgtctga caccgctggc ttgccggaaa aaacgctgaa atttttacgc aataatcagc
    29161 cgcaggtaat ggtgatggat tgcagtcacc cgccgcgcgc ggatgcaccg cgtaatcact
    29221 gtgatttaaa taccgtgctt gcgctgaatc aggttatccg ctcgccacgg gtgattctga
    29281 cccatatcag ccaccagttt gatgcgtggc tgatggaaaa cgcactaccg tcagggtttg
    29341 aggtggggtt tgatgggatg gagattgggg tggcgtgatg agagggaatg tgcgcgctgg
    29401 ccccctcacc ctaaccctct ccccagaggg gcgaggggac cgattgtgct cgatattgaa
    29461 tattgcgctc gttttctccc tctccccatt ggggtgaggg gcgatgcctg ctccataccc
    29521 aacctcatcg cccatactca tcttccattc tccgctcttc atcctccagt tgccgacgct
    29581 cctgatcaag ctggcgctgg cgatcgtcca gctgcctgcg gcgatcttca aactggcggc
    29641 ggcggtcgtc atattgtctg cgccgatcgt cgctcacttc acgctgccag ccgtcgtcgc
    29701 gcgaatcttc atagtctcgc ccacggtcag ggttataagc gtcattaatc gcctgctgaa
    29761 tattaccaat agtatcgtcg ataatatcag cctgggccgg aacggtggac agcgtgagca
    29821 gggtgaataa aagaaatagc ggaaagcgtt tcattagcca acctcaaaaa gaaactctat
    29881 ccacattagt cattactcat ccatgcaagt agtggatgaa tctcaatttc tccgctgctc
    29941 tattgccgta atcgcctcca cgcgttgttg atgacgaccg ccttcgtact gtgcgcccag
    30001 ccacgcatcc acaatcattt ttgccagttc gaggccaacc actcgtgaac caaaagccag
    30061 tgcaacttca ctggcataat gcggataatc agtgcgctct gacgacctgg tgtctttatc
    30121 aatcacctca ctcattgacc atgaataaaa ggctggcaca gcaacgcaga atcctgcggt
    30181 gccagcgaca tgaccgccca ttgctccggt acgtttttta aaaagaaaca ccggttgcat
    30241 tgataaattg tgctcagtat ttgccgaagt acaatatata taataaatat ataaaacacc
    30301 tcatattatt tacagagata ataaattcgc gtaattttcc ctcttcagca caaatgccta
    30361 ataaaatgga tcgttcagct cacacttcca cttattaaag tcgcaaattc ccaattatga
    30421 taaccccagc tttaaatgac gtattttgca tggattacat atttacgaca taaatgataa
    30481 atggagattt acatgaataa ctctcggtta ttccgtttga gcagaattgt tattgcgtta
    30541 actgccgcca gcggcatgat ggtaaatacc gctaacgcga aagaggaagc gaaagccgcc
    30601 actcaatata cccaacaggt taatcagaat tacgccaaat cattaccgtt tagcgatcgt
    30661 caggattttg acgacgctca gcgtggattt atcgctccgc tgctggatga aggtattctg
    30721 cgcgatgcta atggcaaacc atattatcgc ggggaagatt ataaatttga tatcaatgcg
    30781 cccgcgcccg aaaccgttaa ccctagcctg tggcgtcagt cgcagttaaa cggtatttcc
    30841 ggcctgttta aagtcaccga cagaatgtac caggttcgcg gtcaggatat ctccaacatc
    30901 acctttatcg aaggtgatac cgggattatt gtcatcgacc cgctggtgac gccaccgagc
    30961 gcaaaagccg cccttgacct ttacttccaa aatcgcccgc aaaaaccgat tgttgcggtt
    31021 atttataccc acagccatac cgaccactat ggcggcgtga aaggcattat ctctgaagct
    31081 gatgtgaaat ccggtaaagt gcaggttatc gcccctgctg gctttatgga cgaagccatc
    31141 agcgaaaacg tactggcggg taatattatg agccgccgtg cactttactc ctacggcctg
    31201 ctgctggcgc ataaccctca gggtaacatc ggcaatggtc ttggcgtaac gctggcatcg
    31261 ggctacccga gcatcatcgc accgaacaaa accatcacca aaaccggtga gaagatgatt
    31321 atcgacggcc tggagtttga cttcctgatg accccaggta gcgaagcacc agccgaaatg
    31381 cacttctata ttccggccct gaaagcgctg tgtaccgccg agaacgccac gcataccctg
    31441 cacaacttct acactctgcg cggcgcgaaa acccgcgaca ccagcaagtg gaccgagtat
    31501 ctgaacgaaa cgctggatat gtggggtaac gacgcggaag tcctgtttat gccgcacacc
    31561 tggccggtct ggggcaataa gcatatcaat gattatattg gtaaatatcg cgatactatc
    31621 aagtacattc acgaccagac cctgcacctg gcgaaccagg gctacaccat gaatgaaatc
    31681 ggcgacatga ttaaactgcc gcctgcactt gccaataact gggccagccg tggctattac
    31741 ggttctgtca gccacaacgc ccgcgcggtg tataacttct atcttggcta ttacgacggt
    31801 aacccggcta acctgcatcc gtatggtcag gtggagatgg gtaaacgtta cgtgcaggcg
    31861 ctgggcggtt ctgcccgtgt catcaacctg gcgcaagaag cgaacaagca aggtgattac
    31921 cgctggtcgg cagaactgct gaaacaggtg attgccgcca acccgggtga ccaggtcgcg
    31981 aagaatctgc aagcgaataa ctttgaacag ctgggctatc aggccgagtc cgccacctgg
    32041 cgcggtttct acctgaccgg cgcgaaagag ctgcgcgaag gggtgcataa gttcagccac
    32101 ggcaccatcg gttccccgga caccattcgc gggatgtcgg tcgaaatgct gttcgacttt
    32161 atgtccgttc gcctcgatag cgcgaaagcc gcgggtaaaa atatcagcct gaacttcaat
    32221 atgagcaatg gcgataacct caacctgacg ctgaacgata gcgtgcttaa ctaccgtaaa
    32281 acactgcaat cccaagctga cgcctctttc tacatcagcc gtgaagatct gcacgccgtg
    32341 ctgaccgggc aagccaaaat ggcggatctg gtaaaagcga agaaagccaa aattattggc
    32401 aatggcgcga aactggaaga aattatcgcc tgtctggata atttcgattt gtgggtgaat
    32461 atcgtaaccc caaattaatg tcactcgctt ttgggggcgc tgaagccccc aaaagcattt
    32521 attttaaaaa tatatacagc caattaataa ataaaatata taagttatga atgcggtgac
    32581 tgaataaagc tatttttatt catcgagaaa atagccatac tgacgccttc aatggatatt
    32641 gatattcact acagatattg ttcgcagcag acaggatgta tttaatatgc caacagttct
    32701 ctctcgcatg gcgatgcaac tcaaaaaaac agcctggata attcccgtct tcatggtttc
    32761 gggatgctca ttatctccgg caatcccggt gatcggtgct tattatccca gctggttttt
    32821 ctgcgccatt gccagcctta ttttgatgct catcacgagg cgagttattc agcgggcaaa
    32881 tatcaatctg gcatttgtcg gaattattta taccgccctt tttgctctct acgccatgct
    32941 gttctggctg gcatttttct aatctatttg agacgcgctc atggaaagta cgccgaaaaa
    33001 agctcctcgc agtaaattcc ctgctctgtt agtggttgca ttggcgctgg ttgcccttgt
    33061 tttcgttatc tggcgcgtag acagtgcgcc atcaactaat gacgcttacg cgtcagcaga
    33121 taccattgat gtggtgccgg aagtcagcgg ccgcattgta gaactggcgg tcaccgacaa
    33181 ccaggcagtc aaacagggcg atttgctgtt ccgcattgac ccgcgcccgt acgaagccaa
    33241 tctggcgaaa gctgaagcct ccctcgcggc gctggataag caaattatgc tcacccagcg
    33301 tagcgttgat gcgcaacagt ttggtgccga ctcgattaat gccacggtag aaaaagcccg
    33361 tgccgccgcg aaacaggcca cagatacatt acgccgtacc gagccattac tgaaagaggg
    33421 ttttgtctca gcggaagacg ttgaccgtgc aagaactgcg cagcgcgccg cagaagccga
    33481 tcttaatgcc gtattgttac aggcgcagtc agccgccagc gccgtcagcg gcgtcgatgc
    33541 gctggtggcg caacgtgcgg cggtcgaagc ggatattgcc ctgaccaaac tgcatctgga
    33601 aatggcgacc gttcgcgcgc cgtttgatgg ccgggtcatt tccctcaaaa cctccgtcgg
    33661 gcaatttgct tctgccatgc gccctatttt taccctaatc gacactcgtc actggtacgt
    33721 gatcgccaac ttccgcgaaa ccgatctgaa aaatattcgc tcaggtacac ccgcaacgat
    33781 tcgcctgatg agtgacagcg gcaaaacctt cgagggtaaa gtggattcga ttggctacgg
    33841 cgtgctaccg gatgacggcg gcctggtgct gggcggcctg ccgaaagttt ctcgctctat
    33901 taactgggtc cgcgttgccc agcgttttcc ggtcaaaatc atggtcgata aacctgaccc
    33961 ggaaatgttc cgcatcggcg cttcggcagt cgctaatctt gagccgcaat aatgagcgcg
    34021 ctcaactccc tgccattacc gatggtcagg ctgctggcgt tctttcatga agagttaagc
    34081 gagcggcgac ccggccgcgt gccgcagatc gtgcaactct gggtaggctg cctgctggtg
    34141 attctgatct cgatgacttt tgagatccct tttgtggcgt tatcgctggc agtgctgttt
    34201 tacggtattc agtcgaacgc gttttacacc aaatttgtgg cgatcttgtt tgtggttgcc
    34261 acggtgctgg agatcggcag cctgtttttg atctacaaat ggtcatacgg cgaaccgttg
    34321 atccgattga tcatcgccgg gccgatcctg atgggccgca tgtttttgat gcgcacccat
    34381 cggctgggac tggtcttttt cgctgtcgcc attgtcgcca tttacgggca aaccttcccc
    34441 gccatgctcg actatccgga agtggtcgtg cgcttaacgc tgtggtgtat cgttgttggc
    34501 ctctatccaa ccttgctgat gacgttaatc ggcgtgctgt ggtttcccag tcgtgccatt
    34561 tcgcaaatgc atcaggcgct taatgatcgg cttgatgatg ccattagtca cctgacagac
    34621 agcctcgcac cgctacccga aacgcggatt gaaagagagg cgctggcgct gcaaaaactg
    34681 aatgtctttt gtctcgcgga cgatgccaac tggcgaactc aaagcgcatg gtggcaaagc
    34741 tgcgtggcaa cggtaaccta catttactcg acgctgaatc gctacgatcc cacctctttt
    34801 gctgattctc aggcaattat tgaatttcgg caaaaattag cctcagaaat caacaagctg
    34861 cagcatgccg ttgctgaagg tcagtgctgg caaagcgact ggcggatcag tgaaagtgaa
    34921 gcggtggcgg cacgggaatg taacctggag aatatctgcc agacgttgtt acaactgggc
    34981 cagatgaacc cgaatacgcc gccaacgccc gccgccaaac cgccatcaat ggtcgccgat
    35041 gcttttacca atccagacta tatacgctac gcggtaaaaa cattgctcgc ctgtttgatc
    35101 tgttacacct tttacagcgg cgtggactgg gaaggcattc acacctgtat gctgacctgc
    35161 gtgatcgtct ctaatccaaa tgtcggttcg tcgtaccaga agatggtgct gcgttttggc
    35221 ggggcctttt gcggcgcgat tctggcgctg ttattcacgc tactggtcat gccctggctg
    35281 gacaatattg tcgaattgct gtttgtgctg gcaccgattt tcctgttggg cgcatggatt
    35341 gccaccagct ctgaacgctc ttcttatatc ggcacacaga tggtggtcac cttcgcgctc
    35401 gccacgctcg aaaacgtttt tggcccagtg tacgacctgg tggaaattcg cgatcgcgcc
    35461 ctgggtatca tcattggtac cgtggtgtcc gcggtgattt acacctttgt ctggcctgaa
    35521 agtgaagcgc gcacgctgcc gcaaaaactg gctggcgcgc tgggtatgtt aagtaaagta
    35581 atgcggatcc cacgccagca ggaagtcacg gctctgcgca cttatctgca aattcgtatc
    35641 ggtctgcatg cggcgtttaa tgcctgtgaa gagatgtgcc aacgcgtggc gctggagcgt
    35701 caactggaca gcgaagaacg cgccttactg attgaacgtt cgcaaacggt tattcgtcag
    35761 ggccgcgata tccttcacgc ctgggatgcc acctggaatt cggcgcaggc gctggataac
    35821 gcactacagc cggacagagc aggtcagttt gccgacgccc tggaaaaata cgctgccggt
    35881 ctggcaaccg cactcagccg ttctcctcaa ataacgcttg aagagacacc cgcctcgcag
    35941 gccatcctgc ccaccttatt aaaacaggag caacacgtct gccagctttt cgcccgcttg
    36001 ccagactgga cagccccggc attaacgccc gccacggaac aggcacaagg agccacgcaa
    36061 tgatcaatcg tcaactttca cgtctgctgt tgtgcagcat tctcggcagc acgacgctga
    36121 tttccggctg tgccttggta cgcaaggatt ctgcgcctca tcaacagctc aaaccggaac
    36181 aaatcaaact ggccgatgat attcatcttg ccagctccgg ctggccgcag gcgcagtggt
    36241 ggaaacaact caatgacccg tagctggatg cgctgatcca acggacgcta agtggttcac
    36301 acaccctcgc cgaagcgaaa ctgcgggaag aaaaagcgca gtcccaggcc gatttgttag
    36361 atgccggttc acaattacag gtcgcagcgt tagggatgct caaccgccaa cgcgtctcgg
    36421 cgaacggctt tttaagccct tatgcgatgg atgcgcccgc actgggtatg gacgggccgt
    36481 actatacgga agccacagta ggtttgtttg ccggactgga tcttgatttg tggggtgtgc
    36541 atcgctcagc ggttgccgcc gccattggcg cgcataatgc cgcgctggca gaaaccgcag
    36601 cagtagagct atcgctgacc acgggcgtag cgcagcttta ttacagtatg caggccagct
    36661 atcagatgct cgatctgtta gaacaaactc gcgatgtgat tgattacgcg gtgaaagcgc
    36721 accagagtaa agtggcgcac ggtctggaag cacaagtgcc tttccacggc gcgcgggcac
    36781 agattctggc ggtcgataaa caaattgctg ccgtcaaagg gcaaatcacc gaaacgcgag
    36841 aatctctgcg tgcattgatt ggcgcggggg ccagcgatat gccggagatc aaaccggtgg
    36901 cattaccacg agtccagacc ggcattccgg cgacactctc ttatgagttg ctcgccagac
    36961 gcccggatct gcaagccatg cgctggtatg ttcaggcgtc attagatcag gtggattccg
    37021 cgcgggcgct gttctacccg agctttgata tcaaagcgtt tttcggcctt gatgccatcc
    37081 acctggatac cttattcaaa aaaaccagtc gccagttcaa cttcatcccg ggtctgaaat
    37141 tgccgctgtt tgacggtgga cggttgaatg ccaatctcga aggcacgcgc gccgccagca
    37201 acatgatgat tgaacgttac aaccagtcag tactgaacgc ggtgcgtgac gttgccgtca
    37261 acggcacgcg tctgcaaacg ctcaacgacg agcgagaaat gcaggctgaa cgcgtggaag
    37321 caacgcgctt cacccagcgc gctgccgagg ccgcctatca gcgcggctta accagccgct
    37381 tacaggccac tgaagcccgg ttgccagtac ttgccgaaga gatgtcatta ctgatgctgg
    37441 acagccgccg ggtgatccaa agcattcagt tgatgaaatc gctgggcggc gggtatcagg
    37501 cggctcccgt cgtcgagaaa aaataaaatg tctgccgcgt gatggctgtc acgcggtatt
    37561 tcgtttcgtc acgtcaaaac tgacgacagc ctgtttttcg tcagagtttt gaataaatag
    37621 tgcccgtaat atcagggaat gaccccaaat aaaatgtggc ataaaagatg catactgtag
    37681 tcgagagcgc gtatgcgtga tttgattaac tggagcgaga ccgatgaaaa aagtcgtcac
    37741 ggtttgcccc tattgcgcat caggttgcaa aatcaacctg gtcgtcgata acggcaaaat
    37801 cgtccgggcg gaggcagcgc aggggaaaac caaccagggt accctgtgtc tgaagggtta
    37861 ttatggctgg gatttcatta acgataccca gatcctgacc ccgcgcctga aaacccccat
    37921 gatccgtcgc cagcgtggcg gcaaactcga acctgtttcc tgggatgagg cactgaatta
    37981 cgttgccgag cgcctgagcg ccatcaaaga gaagtacggt ccggatgcca tccagacgac
    38041 cggctcctcg cgtggtacgg gtaacgaaac caactatgta atgcaaaaat ttgcgcgcgc
    38101 cgttattggt accaataacg ttgactgctg cgctcgtgtc tgacacggcc catcggttgc
    38161 aggtctgcac caatcggtcg gtaatggcgc aatgagcaat gctattaacg aaattgataa
    38221 taccgattta gtgttcgttt tcgggtacaa cccggctgat tcccacccaa tcgtggcgaa
    38281 tcacgtaatt aacgctaaac gtaacggggc gaaaattatc gtctgcgatc cgcgcaaaat
    38341 tgaaaccgcg cgcattgctg acatgcacat tgcactgaaa aacggctcga acatcgcgct
    38401 gttgaatgcg atggaccatg tcattattga agaaaatctg tacgacaaag cgttcgtcgc
    38461 ttcccgtaca gaaggctttg aagagtatcg taaaatcgtt gaaggctaca cgccggagtc
    38521 ggttgaagat atcaccggcg tcagcgccag tgagattcgt caggcggcac ggatgtatgc
    38581 ccaggcgaaa agcgccgcca tcctgtgggg catgggtgta acccagttct accagggcgt
    38641 ggaaaccgtg cgttctctga ccagcctcgc gatgctgact ggtaacctcg gtaagccgca
    38701 tgcgggtgta aacccggttc gtggtcagaa caacgtgcag ggtgcctgcg atatgggcgc
    38761 gctgccggat acgtatccgg gataccagta cgtgaaagat ccggctaacc gcgagaaatt
    38821 cgccaaagcc tggggcgtgg aaagcctgcc tgctcatacc ggttatcgca tcagcgagct
    38881 gccgcaccgc gcagcgcatg gcgaagtgcg tgccgcgtac attatgggcg aagatccgct
    38941 gcaaactgac gcggagctgt cggcagtacg taaagccttt gaagatctgg aactggttat
    39001 cgttcaggac atctttatga ccaaaaccgc gtcggcggcg gatgtcattt tgccgtcaac
    39061 gtcgtggggc gagcatgaag gcgtgtttac tgcggctgac cgtggcttcc agcgtttctt
    39121 caaagcagtt gaaccgaaat gggatctgaa aacggactgg caaatcatca gtgaaatcgc
    39181 cacccgtatg ggttatccga tgcactacaa caacacccag gagatctggg atgagttgcg
    39241 tcatctgtgc ccggatttct acggtgcgac ttacgagaaa atgggcgaac tgggcttcat
    39301 tcagtggcct tgccgcgata cttcagatgc cgatcagggg acttcttatc tgtttaaaga
    39361 gaagtttgat accccgaacg gtctggcgca gttcttcacc tgcgactggg tagcgccaat
    39421 cgacaaactc accgacgagt acccgatggt actgtcaacg gtgcgtgaag ttggtcacta
    39481 ctcttgccgt tcgatgaccg gtaactgtgc ggcactggcg gcgctggctg atgaacctgg
    39541 ctacgcacaa atcaataccg aagacgccaa acgtctgggt attgaagatg aggcattggt
    39601 ttgggtgcac tcgcgtaaag gcaaaattat cacccgtgcg caggtcagcg atcgtccgaa
    39661 caaaggggcg atttacatga cctaccagtg gtggattggt gcctgtaacg agctggttac
    39721 cgaaaactta agcccgatta cgaaaacgcc ggaatacaaa tactgcgccg tgcgcgtcga
    39781 gccgatcgcc gatcagcgcg ccgccgagca gtacgtgatt gacgagtaca acaagttgaa
    39841 aactcgcctg cgcgaagcgg cactggcgta atacagtcct ttctacagcc tcctttcgga
    39901 ggctgttttt ttatccattc gaactcttta tactggttac tccctaccca atcgtattat
    39961 caaaatgaaa aaaattatcg cattgatgtt gtttttgaca ttctttgccc acgccaacga
    40021 ctccgagcct ggcagccagt atttaaaggc agcagaggcc ggggaccgac gcgcacaata
    40081 ttttcttgcc gacagctggt ttagctccgg cgatttgagc aaagccgaat attgggcaca
    40141 gaaagccgcc gacagcggtg atgctgatgc ctgcgcgctg ctggcgcaga tcaaaatcac
    40201 caatccggtc agtctggact atccacaagc aaaagttctt gcagagaaag cggcgcaagc
    40261 gggcagtaaa gaaggtgaag taacgctggc gcatattctg gtaaatactc aagcgggtaa
    40321 accggattat ccaaaggcta tttcgctgtt agaaaacgcc tcggaagatc tggagaacga
    40381 ctctgccgtc gatgcccaaa tgctgcttgg tttgatttac gccaacggcg tgggcattaa
    40441 ggccgacgat gacaaggcaa cctggtattt caaacgcagc tctgcaattt cccgaaccgg
    40501 ttattccgag tactgggcgg gaatgatgtt cttaaacggt gaagaaggtt ttatcgagaa
    40561 gaacaagcaa aaggcgctgc actggttgaa cctgagctgt atggaagggt ttgataccgg
    40621 gtgtgaagag tttgaaaaat taaccaacgg ttaaggtagg cctgataaga cgcggcaagc
    40681 gtcgcatcag gcattcacac attgccgggt ggatatcccc ggcaatcttc aattattgat
    40741 ccgcagtttt atcaaatttg cccagcactt cacgctcata agccagcgcc tttttgcggt
    40801 caaatttgtg ttcccacttg gcaatcacca gcaccgccag cgcattaccc accacgttca
    40861 gcgcagtacg cgccatgtcg aggatacggt caacaccagc aataaacgcc agaccttcca
    40921 gcggaatacc tacgctaccc agcgttgcca gcagcaccac aaacgacacg cccggcacgc
    40981 cagcaatccc tttcgaggtc accatcagcg tcagcaccag aatgatttcc tgccagatgg
    41041 acaggtcaat gccatacagc tgagcgatga aaatagcggc aatactttga tacagcgtcg
    41101 aaccatcaag gttaaaagag taaccggtcg gcaccacgaa gctggtgatc gacgccggtg
    41161 ctccgtaggc ttccatcttc tcaataattc gcggcagtac gctttcagag ctggcagtgg
    41221 agtacgccag aatcagctca tctttcagaa tacgaatcag gatccagacg cttaacccgc
    41281 acaggcgcgc cacaattccc agcactacca gcgcgaagaa cagaatggcg aaatgcacca
    41341 gcagcaccag tttcgccagt ggccacagag aagagaaacc aaagttagcc accgtcaccg
    41401 caatcagcgc aaacacaccc accggtgcat aacgcatcac catgtgagtc actttaaaca
    41461 tggtttcaga gatggagcgg aacacggtca ccagcagttc acgatgcgtc gcgggcaggg
    41521 aagaaagccc cagaccaaac agcaccgaga aaaagatgat cggcagcatt tcgcctttcg
    41581 ccatcgacgc cacaatgttc gtcggcacca gcgacaaaat cgtgcccata atgccgtggg
    41641 aactgctttg taccgcttcc gtagtgctct gatatttcga gatatcgacg gtcgccagct
    41701 gcgacatatc caccccggca ccgggctgga agacgttcgc cagagtgatc cccaaaatga
    41761 tggcgacggt ggtgatcacc tcgaagtaaa taatggtttt cgcgccaata cgcccgagct
    41821 gtttggcatc accaacaccc gcgataccca ccaccagcgt ggagatcaca atcggcacaa
    41881 caatcatttt gatcagatgg atgaagatat cacccgccgg agagagcaaa ttgacgacca
    41941 gccagtcgcg gctgtcgcta tggtagtgca ggtagcttcc caggagaatg cccagcacca
    42001 tagcaaacag aatctgccag gccaggctga attttatatt tttcatcaga ataacttcct
    42061 caatggagcg ccgttccccg ttcagaaacg cgagaacgtg acatactgcc agggcatgaa
    42121 ttgtgttgcg ttggtttgta gattttttta acgcttatga tcagtatcat ttgcgcggta
    42181 tgttgcgcaa ccctttaaca acagagcttt tcgctgcatt aatgcataat aacgcgagtt
    42241 tatggcagga ttaatgaata accatttgtt atggaaagat ttttatttgt ttaatctgca
    42301 ataatactga ttagtgatta tttttggcat atgttcattc gattattttt tatgcagctc
    42361 aatgacataa tcttcatcac cagatgatta atccgatctc cgctgcaaca gttttgccat
    42421 attaatcgac tcgaccagct gtgtccggtt gatccggggt gagttgagat ccatcacttt
    42481 ttgccatagt tcgatggctt gcgcgtaatt cgcctgcata aacgcatcgg aagccagcag
    42541 catcagagcg gtgatttcgt tagagtccag cgcgagggct ttgtcgatca ttgcgcaagt
    42601 ctgggcggtc atatgctggc tggcctggta ataaagcacc gtcgccagcg ccgcatacag
    42661 ttcagcgttc tcaccacgta gttgcaacgc ctgacgatac gccagcagcg aattgctgta
    42721 atcgttttgc cacagatagt actcgcccag taacgcccat tgttcgctgt tttgtggatt
    42781 agcgcggatt ttatcctgca atgcctgaag ctgcgcttcc ggggtttgct ggctggcaaa
    42841 ctgatgtagc ggatcgcgct gacgctgata ctccgcacgt accgcctgcc attttgggct
    42901 taacagataa ctgccgacac agaggaatac catcaatatc gtgagtgttg tcagcatttt
    42961 caccggtatt tgaggctgtt tcatcgctta gccctcactc gccagaggat cagtgccatc
    43021 agcaataaca acaccactgg cagcgcccac aacaccagcg tctgacccgt taacggtggg
    43081 ttatagcgaa caaaatctcc gtagcgttcg gtcatccagc caatgatttc gacttcgttc
    43141 ttcccctccg acaccatgct ataaacctga tggcgcatac tgaccgccac cggcgcattg
    43201 gattcgagta agttttgatt ctggcactgc ggacaacgta actggctggc aatatttaac
    43261 gcctgttgct gttgttgcgg attggcgaat tgccaggtgt ctacgacctg agcgcgcgca
    43321 aaacaggtaa ataacagcag tagcgtgaga agccctttat tcatcgcgct tcttcccccg
    43381 ccatccgctt aacaatgcgc ccgcaatcat caacaatcct ccaccccaga tccagcgcac
    43441 accgctttgt acatacaaac gaaaagcgta acgatccgct ccggtttttt cacccatgac
    43501 cgcataccag tcatggatgc cgttccagcg aattgacggt tccatcattt gctgacggcg
    43561 tgcttcataa aaacgccgct ccggcgttaa ttcaccaatg cgttgctgat ggtcaaacag
    43621 cgccactatc gctttttcgc tggtgtaatt gcctttggct tgcagatcga ggcgctcaaa
    43681 acggaaggtg tatcctgcca gcgtcacctg ctgacccggc tgtaaattca ggctgatctc
    43741 ctgacggctg acgctggaaa ccacgatccc tgcggcaaat aacagcacgc ccgcatgagc
    43801 gaccagcgcc ggaagctgcg cgcgtttgcc agagacaaac gtcgccagca caatcaccac
    43861 cagcatcaac agaccaaacg gtaacgtcgc gcggttaaaa tacggcgcgc caacggagag
    43921 gcgtccccag cccagcagac cgtaaatcat cggataaagc gttcccacca gcacgatcag
    43981 tagcactgcg caaaacagca acagcgtagc gaggattaac atttcccgcg ataaccccga
    44041 aaaacgcacc gccgggccac cgtcccgcgc tcgccagcca tacagagcca aagacgcaag
    44101 gctaatcagt gcaaacaggc tgaacaacgg cacggcgcgg acgttatcca gtgcgaacgc
    44161 atgaaccgaa accagaatgc cagaacggac aattaaggtg cccagcagcg acagcatcag
    44221 agtaactatc gccagcaaca gcgaccagtg gcaaaaaatc ccccgctggc gtgtcaggga
    44281 taaactgtgc agcagcgcag tggcagaaag ccagggtaat aaagaggcat tttccaccgg
    44341 atcccagaac caccagccgc cccagccgag ttcgcagtag gcccaccagg aaccgaggat
    44401 gatccccgcc gttaatgcac tccagccagg taacgcccag cgccagcaaa ttcgggcgct
    44461 ggccgcatca aactcgccgc gcagtagact cgccagcgcc acgctcgccg ctaccatcaa
    44521 accgccatag ccaaggtaaa gcagcggtgg atgaaagata agaccgggat gttgcagcat
    44581 cggattgaga tcgcggcctt cgattgctgg tggaaatatc cgcacgaagg gatcggacca
    44641 cagcaccaca aacagtagca gtgcggcgag catgagagat aaaacggcta acgtcagcgg
    44701 aaatagcgga tcggtttgct gccgataatg ccaggcaaac agcgcgctcc aggcggaaag
    44761 cagcagcacc caaagcagca gcgaaccttc atggccgccc cacaccgctg ccagcttgaa
    44821 ttcccacgac agcaggctat agctatgttg cgcgacataa atgaccgaga aatcgctgat
    44881 gagaaaacaa tacgtcagta cgccaaaggc gagcagcagg agcgcgaact gcgccagaat
    44941 gccgatgcaa gtgagtcgca tcatggcagg ccagcgcaac cgcactcccg caaaggccgt
    45001 caacggggtc aacacgttga ccccgagact taacaacaac gccagaaaac cgacttcagg
    45061 aaggaatacg tccagaaacc acctcttttc tggcttacgc caccgtcaac tgtccggcgt
    45121 agagaatgaa aaagcgcagc atcaacacgc cgaccagact ggccccacac gccgccagca
    45181 cggcaggaat gccggaactg cgattgaccc acggtttgag caacattggc acaatcagcc
    45241 ccagcccggc gacaccaagc cagaaccacc acgtccagaa accgccacct aatgccgcca
    45301 ccagcgcacg aactttaccg tcatcgccca gcgccagacc gacaaaaaac gccaccagca
    45361 ggaagatttc accccatacc acggggattt ccatccggtg aacaaagtgc gcttccgtgg
    45421 aatgcgggtt actgcgttgg cgtatcgcca tggcgatcag cgccaccgcc gcaccggacg
    45481 agatgccgga gaagaggaac agcaccggca ggatcgggtt attgaggaac ggatacgatt
    45541 tcagcgccga cagcagaaag ccggtataag cccccagcag caccgccaac accagcatca
    45601 gcgtttccag tccgcgatga acgggcgtca gcaggctcag aaccttttgc acgatcccca
    45661 gcttcggcaa ccagcgttgt tgcagggcaa gcaaatcatg ttcaaagatt ttcgccagcc
    45721 acagcaccag caccaccatg tagagctgaa acagcatcac ccccatcgac atcaccgagg
    45781 taaaactgta gtggaacatc agcttccaga aggtccacgg tcttgtcagg tggaagacga
    45841 ggatcaacag accgaggatc accgcgcccg gcccgacaat cagcgtggtg cgcagcaacg
    45901 tactgtctgc accgcccgcc tgcgggtaga agcgacgtaa cagcacggcc agcgtcacca
    45961 gaccggcaga aataccaatc aaaaacaggt agatggcaat cggccagtcc cacaccagcg
    46021 attcaaaatg aaatgcggaa gtctgcgtca ttggctcacc tccccgtatt taaacggtac
    46081 gcggtacagc ttcggtttgg ttcccagcgc cagcttgtag cggtaagtag gcttctggcg
    46141 cagcagttgc gaaatctcac tgttaggatc gtccagattg ccaaacgtca gcgccttcgt
    46201 cgggcaagcc tcaacacacg cgggcagctt accggcctgc aaattggttt tacggcagaa
    46261 atcgcatttg tccgccgtct tcgtgaccgg atggataaag cgcacgcggt acggacaggc
    46321 ggcgatgcag tactgacaac cgacgcagag atcaggatta acatcgacaa tcccgctagc
    46381 ggcatcacga aaagacgcac cggtcgggca gacgtcaacg cacggcgcat gatcgcagtg
    46441 ctggcaagac ttacggaaga agcgatattt cacatcagga aattcgccct gcggctcgct
    46501 acgaataatc gtcaggcgcg agacgccttc cggcaccttg ttcacttccc gacaagcatc
    46561 catacaggcg gtacagccga tgcataacga ctcgtcatgc accatgccgt aacgcacccc
    46621 attgatattc aacgttttcg ccaccacgcg ccctgcggtg ccgctgacgg ctgccagcac
    46681 gccgacgccg gtgagaaact ggcgacgaga ccaggtcatg gctgctcctt aagcaacgga
    46741 acagacgccg ggttaaagtt cggattggtg cgctgatcgc tgtggcaatc gacgcaaatc
    46801 ttaatccgtc ctttgtcact taacgtctgc atcgtatctt gctgtggatg cagggaatgg
    46861 cagctggcgc acgccacttt ggttacgtgg acatcgtgcg gccagaacgc tttttgcaac
    46921 tgttccggca gatgacagga catacagacg ctgttctgct ctccaacctt gtacatcggc
    46981 tcgttaaagc gcatcacatc tttcacccct tcgcggtgtt gcggtgatgg ctggccgtga
    47041 cagttggtgc aggtgaccgg cagtttgtta ttcgggttga taacggaagc atgtttgcca
    47101 tgcatacctt cggtatctgg tttatgacag tccagacagg cggcatccgg gttacgctgc
    47161 tgggtaactt cgtagcgatc atcagatgcc tgcgccgcag gggtagcggt aattccgttc
    47221 aggctccaca acaggcccga cgccagcacc ccggcagtta ataacgaacg taatacgctc
    47281 atattcactc cattgtgttt tccgcccctt gcgaggcgga acggggttat tggcttaaca
    47341 gaccgttttt acgtgcctgt tcttcccact gcgggatcac cgttttgatg aagtcctgct
    47401 tctcggcctt gatttgttcc atgttcaggc caatggcctg ctgggctttc tctttggttg
    47461 agatatccgg gatctggatt tcatgggtga tgcctttggt cgccagcagg cgcgccagtt
    47521 tggtgcgtgc atccgccgct ttatccatcg ccgtaccgag catccgtaaa ccttcttccg
    47581 gtgcgtgcat atgaatgccg tgggaagcga tcgccagatc ccagcgccac tgggcatgac
    47641 ggatatcgtc ctgaattggc ttcatttcag cttccgtcgc gcctgcatcc agcgctgctt
    47701 tcgcttcgaa gtgagcgtga accagttgat cttcaacctt gattttcagg tcgttaatcg
    47761 actgcttacg ttccgcgacc actttttgca aggcagcttt gtcctgggta tggcagttcg
    47821 cacaagtctg ggcgaagtta tcaaacggat taccaatttt atggtcggta tagagtttgc
    47881 cttcggcgtt ctgcactttt ggcatatggc agtcgataca ggtcacgttg tttttaccgt
    47941 gaatgcccgc tgtccaggtt tcatattccg ggtgctgcgc tttcagcatt ggcgttttcg
    48001 acagggagtt agtccagtca gagaaggcaa ttttgtcgta atactgctcc atattttcga
    48061 ctttcatgcc gtcatcccac gggaatttaa ccgctttgtt tttgccgtcg aagtaatact
    48121 ccacatggca ctgaccgcaa accatcgatt gctggtcgaa acgtccggct ttctcaaaag
    48181 gtttaccaat ggcttccatc gcgcgagccg catacggacg ggaaagggtt aactccggtt
    48241 tgcctttggc gaactctggt gaggcggtgt tatggcagtc ggcacaacct aagttgttga
    48301 cgatttccgg gccgccgcgc gcccacttac cgtggaagta gccatcttcg ccgtctttct
    48361 ggatcagacg cgccacatcc gggcttttac aactccagca cgccatcggt agcggaccat
    48421 cttcagcgtt tttcggcgca ccggtacgca gggtttcacg cacatcggtc acagcaaagg
    48481 catgtccacg cggcttgttg taatcgcgcg agaagggata ccccgcccac aggatcacca
    48541 gccgtggatc ttccgccagg gcgtcaacac gctctgactg ttccgaggtg gctttccagg
    48601 agagatattg atcgggatgc tgcggggcaa aggtttcatt cttcgcttcc acagttacag
    48661 gttttgcggg agctgccgtt tgttcagcgt gaacagaagt gaaaaagaaa aaaggaatca
    48721 ataagctgaa gatacggcgt gcgtttattt ttatccttgt cataggggct tcatccgaat
    48781 tgcgccattg ttacaatggc ggtttttatt gtttttcacg acagtaaccg cacctacact
    48841 gtcatgacat tgctcgcccc tatgtgtaac aaataaccac actgtgaatg ttgtctttaa
    48901 tcaattgtaa gtgcatgtaa aataccactt tagagttagt tagtatcttc ctctttttca
    48961 acagcaatgc ataactgcat gttcctcaaa gaattaatca acttttgttg ctgaccttca
    49021 aaaattaccc tgccgtttat ttgcacaatt ctacttttgc gtgatctgtc gcccaaatac
    49081 taaacaaaac tgccaatacc cctacattta acgcttatgc cacatattat taacacccta
    49141 caaggagaac aaaagcatga gccaaattca caaacacacc attcctgcca acatcgcaga
    49201 ccgttgcctg ataaaccctc agcagtacga ggcgatgtat caacaatcta ttaacgtacc
    49261 tgataccttc tggggcgaac agggaaaaat tcttgactgg atcaaacctt accagaaggt
    49321 gaaaaacacc tcctttgccc ccggtaatgt gtccattaaa tggtacgagg acggcacgct
    49381 gaatctggcg gcaaactgcc ttgaccgcca tctgcaagaa aacggcgatc gtaccgccat
    49441 catctgggaa ggcgacgacg ccagccagag caaacatatc agctataaag agctgcaccg
    49501 cgacgtctgc cgcttcgcca ataccctgct cgagctgggc attaaaaaag gtgatgtggt
    49561 ggcgatttat atgccgatgg tgccggaagc cgcggttgcg atgctggcct gcgcccgcat
    49621 tggcgcggtg cattcggtga ttttcggcgg cttctcgccg gaagccgttg ccgggcgcat
    49681 tattgattcc aactcacgac tggtgatcac ttccgacgaa ggtgtgcgtg ccgggcgcag
    49741 tattccgctg aagaaaaacg ttgatgacgc gctgaaaaac ccgaacgtca ccagcgtaga
    49801 gcatgtggtg gtactgaagc gtactggcgg gaaaattgac tggcaggaag ggcgcgacct
    49861 gtggtggcac gacctggttg agcaagcgag cgatcagcac caggcggaaa agatgaacgc
    49921 cgaagatccg ctgtttattc tctacacctc cggttctacc ggtaagccaa aaggtgtgct
    49981 gcatactacc ggcggttatc tggtgtacgc ggcgctgacc tttaaatatg tctttgatta
    50041 tcatccgggt gatatctact ggtgcaccgc cgatgtgggc tgggtgaccg gacacagtta
    50101 cttgctgtac ggcccgctga cctgcggtgc gaccacgctg atgtttgaag gcgtacccaa
    50161 ctggccgacg cctgcccgta tggcgcaggt ggtggacaag catcaggtca atattctcta
    50221 taccgcaccc acggcgatcc gcgcgctgat ggcggaaggc gataaagcga tcgagggtac
    50281 cgaccgttcg tcgctgcgca ttctcggttc cgtgggcgag ccaattaacc cggaagcgtg
    50341 ggagtggtac tggaaaaaaa tcggcaacga gaaatgtccg gtggtcgata cctggtggca
    50401 gaccgaaact ggcggtttca tgatcacgcc gctgcctggc gctaccgagc tgaaagccgg
    50461 ttcggcaaca cgtccgttct tcggcgtgca accggcgctg gtcgataacg aaggtaaccc
    50521 gctggaaggc gctaccgaag gcagcctggt gatcaccgac tcctggccgg gtcaggcgcg
    50581 tacgctgttt ggcgatcacg aacgttttga acagacctac ttctccacct tcaaaaatat
    50641 gtatttcagc ggcgacggcg cgcgtcgcga tgaagatggc tattactgga tcaccgggcg
    50701 tgtggacgac gtgctgaacg tctccggtca ccgtctgggg acggcagaga ttgagtcggc
    50761 gctggtggcg catccgaaga ttgccgaagc cgccgtagta ggtattccgc acaatattaa
    50821 aggtcaggcg atctacgcct acgtcacgct taatcacggg gaggaaccgt caccagaact
    50881 gtacgcagaa gtccgcaact gggtgcgtaa agagattggc ccgctggcga cgccagacgt
    50941 gctgcactgg accgactccc tgcctaaaac ccgctccggc aaaattatgc gccgtattct
    51001 gcgcaaaatt gcggcgggcg ataccagcaa cctgggcgat acctcgacgc ttgccgatcc
    51061 tggcgtagtc gagaagctgc ttgaagagaa gcaggctatc gcgatgccat cgtaacccac
    51121 aattgccgga tgcgagtcgg taacggtttg taggcctgat aagacgcgac agcgtcgcat
    51181 caggcattga ttgccggatg cggcgtaaac gccttatccg gcctacattc ggcaagggtt
    51241 acccgagcgt taaccttctc ccataaggga gcgggaatta aaacaatccc tacattacct
    51301 ctggagaatc tgtgatgaat ggcactattt atcagcggat agaagacaat gcgcatttca
    51361 gggagttagt cgaaaaacgg caacggtttg ccaccatcct gtcgattatt atgctggcag
    51421 tttatatcgg ctttatttta ctgatcgcct tcgcgcccgg ctggctggca cgccgctgaa
    51481 tccgaacacc agcgtcacac gcggtattcc aattggtgtt ggagtgattg tgatctcctt
    51541 tgttctcacc ggtatctaca tctggcgggc gaacggcgaa ttcgaccgtc ttaataatga
    51601 agtcctgcat gaggtacaag catcatgaaa agagttctga cggcgcttgc cgccacactc
    51661 cctttcgcag ctaacgccgc ggatgctatt agcggggccg tagagcgcca gccaacgaac
    51721 tggcaggcga ttattatgtt cctgattttc gtcgtgttta cgctcggcat tacctactgg
    51781 gcatcaaaac tcgtacgttc tcgtagcgac tactacaccg caggcggcaa tatcactggc
    51841 ttccagaacg ggctggcgat tgccggggac tatatgtccg ccgcctcatt cttggggatc
    51901 tccgcgctgg tgtttacctc cggctatgac ggcttaattt actcgctggg cttcctggtg
    51961 ggctggccga tcattttgtt cctgattgcc gaacgtctgc gtaacctggg gcgctacacc
    52021 tttgccgatg tggcctctta ccgtctgaaa caagggccga ttcgtattct ttcggcctgt
    52081 ggttctctgg tggtggtggc gctttacctt atcgcccaga tggtgggcgc aggtaaactg
    52141 atcgagctgc tgtttggcct taactatcac attgcggtgg tgctggtcgg cgtgctgatg
    52201 atgatgtacg tcctgttcgg cggcatgctg gcgaccactt gggtacagat tatcaaagcc
    52261 gtgctgttgc tgttcggtgc cagctttatg gcctttatgg tgatgaaaca cgtcggcttt
    52321 agcttcaaca atctgttcag tgaagcgatg gcggtacacc cgaaaggtgt cgacatcatg
    52381 aagccgggcg ggctggtgaa agatccgatc tccgcactct ctctgggtct gggactgatg
    52441 tttggtacgg cgggcttgcc gcacattctg atgcgcttct ttacagtcag cgatgcccgc
    52501 gaagcacgta agagcgtgtt ctacgccacc gggtttatgg gctacttcta tattctgacc
    52561 tttattatcg gcttcggcgc gatcatgctg gttggtgcga atccggaata taaagacgcg
    52621 gcgggccatc tgattggtgg taacaacatg gcggccgttc acctggcgaa tgcagtgggc
    52681 ggcaacctgt tcctcggttt tatttcagcg gttgctttcg ccactatcct cgcggtggtt
    52741 gcgggtctga cgctggcggg cgcatccgcg gtttcgcatg acttgtacgc taacgtcttc
    52801 aaaaaaggcg cgaccgaacg tgaagagctg cgggtatcaa aaatcaccgt actgatcctc
    52861 ggcgtgattg cgattatcct cggcgtgctg tttgagaatc agaacatcgc ctttatggtg
    52921 gggctggcgt ttgccatcgc ggcgagctgt aacttcccga tcattctgct ttctatgtac
    52981 tggtcgaaac tgaccacgcg tggcgcgatg atgggtggct ggctggggct gattaccgca
    53041 gtagtactga tgatcctcgg cccgacgatt tgggtacaga tccttggtca cgaaaaagcc
    53101 atcttcccgt atgaataccc ggcgctgttc tctatcaccg tggcattcct cggcatctgg
    53161 ttcttctcgg caaccgataa ctcagcggaa ggggcgcgcg agcgtgaact gttccgcgcg
    53221 cagtttatcc gctcccagac cggctttggc gttgagcaag gccgcgcaca ttaatctctc
    53281 cccttccccg gtcgcctgac cggggaatac tcttcctctc cagcatgcat caccttttcc
    53341 caaaatatta aacaaataaa ctcattaaaa aatgagcgat ttttgacagt cgtagaaaat
    53401 gata
//
LOCUS       DAESHI010000018        51278 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.018, whole genome shotgun sequence.
ACCESSION   DAESHI010000018 DAESHI010000000
VERSION     DAESHI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 51278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51278
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.018"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            228..1130
                     /locus_tag="KW011_001088"
     CDS             228..1130
                     /locus_tag="KW011_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH7659488.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPVFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            1277..2497
                     /locus_tag="KW011_001089"
     CDS             1277..2497
                     /locus_tag="KW011_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HBH7659489.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKNSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPV
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQNLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            2470..3099
                     /locus_tag="KW011_001090"
     CDS             2470..3099
                     /locus_tag="KW011_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBH7659490.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVICLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(3100..4260)
                     /gene="ybdL"
                     /locus_tag="KW011_001091"
     CDS             complement(3100..4260)
                     /gene="ybdL"
                     /locus_tag="KW011_001091"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HBH7659491.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            4369..5457
                     /gene="hcxA"
                     /locus_tag="KW011_001092"
     CDS             4369..5457
                     /gene="hcxA"
                     /locus_tag="KW011_001092"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HBH7659492.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(5467..5664)
                     /locus_tag="KW011_001093"
     CDS             complement(5467..5664)
                     /locus_tag="KW011_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBH7659493.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(5846..7951)
                     /gene="cstA"
                     /locus_tag="KW011_001094"
     CDS             complement(5846..7951)
                     /gene="cstA"
                     /locus_tag="KW011_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HBH7659494.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(8132..8545)
                     /gene="entH"
                     /locus_tag="KW011_001095"
     CDS             complement(8132..8545)
                     /gene="entH"
                     /locus_tag="KW011_001095"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HBH7659495.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(8548..9294)
                     /gene="entA"
                     /locus_tag="KW011_001096"
     CDS             complement(8548..9294)
                     /gene="entA"
                     /locus_tag="KW011_001096"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HBH7659496.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(9294..10151)
                     /gene="entB"
                     /locus_tag="KW011_001097"
     CDS             complement(9294..10151)
                     /gene="entB"
                     /locus_tag="KW011_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HBH7659497.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(10165..11775)
                     /gene="entE"
                     /locus_tag="KW011_001098"
     CDS             complement(10165..11775)
                     /gene="entE"
                     /locus_tag="KW011_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HBH7659498.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(11785..12960)
                     /gene="entC"
                     /locus_tag="KW011_001099"
     CDS             complement(11785..12960)
                     /gene="entC"
                     /locus_tag="KW011_001099"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HBH7659499.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            13335..14300
                     /gene="fepB"
                     /locus_tag="KW011_001100"
     CDS             13335..14300
                     /gene="fepB"
                     /locus_tag="KW011_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH7659500.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSVLALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            complement(14381..15631)
                     /gene="entS"
                     /locus_tag="KW011_001101"
     CDS             complement(14381..15631)
                     /gene="entS"
                     /locus_tag="KW011_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HBH7659501.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            15742..16746
                     /gene="fepD"
                     /locus_tag="KW011_001102"
     CDS             15742..16746
                     /gene="fepD"
                     /locus_tag="KW011_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HBH7659502.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            16743..17735
                     /gene="fepG"
                     /locus_tag="KW011_001103"
     CDS             16743..17735
                     /gene="fepG"
                     /locus_tag="KW011_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HBH7659503.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            17732..18547
                     /gene="fepC"
                     /locus_tag="KW011_001104"
     CDS             17732..18547
                     /gene="fepC"
                     /locus_tag="KW011_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HBH7659504.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(18544..19677)
                     /gene="fepE"
                     /locus_tag="KW011_001105"
     CDS             complement(18544..19677)
                     /gene="fepE"
                     /locus_tag="KW011_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HBH7659505.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(19893..23774)
                     /gene="entF"
                     /locus_tag="KW011_001106"
     CDS             complement(19893..23774)
                     /gene="entF"
                     /locus_tag="KW011_001106"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HBH7659506.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     TDLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(23771..23989)
                     /gene="ybdZ"
                     /locus_tag="KW011_001107"
     CDS             complement(23771..23989)
                     /gene="ybdZ"
                     /locus_tag="KW011_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HBH7659507.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(23992..25194)
                     /gene="fes"
                     /locus_tag="KW011_001108"
     CDS             complement(23992..25194)
                     /gene="fes"
                     /locus_tag="KW011_001108"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HBH7659508.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            25437..27677
                     /gene="fepA"
                     /locus_tag="KW011_001109"
     CDS             25437..27677
                     /gene="fepA"
                     /locus_tag="KW011_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HBH7659509.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            27852..28472
                     /gene="entD"
                     /locus_tag="KW011_001110"
     CDS             27852..28472
                     /gene="entD"
                     /locus_tag="KW011_001110"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HBH7659510.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(28594..28746)
                     /gene="hokE"
                     /locus_tag="KW011_001111"
     CDS             complement(28594..28746)
                     /gene="hokE"
                     /locus_tag="KW011_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HBH7659511.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(29097..29249)
                     /gene="hokE"
                     /locus_tag="KW011_001112"
     CDS             complement(29097..29249)
                     /gene="hokE"
                     /locus_tag="KW011_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HBH7659512.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            29689..30807
                     /locus_tag="KW011_001113"
     CDS             29689..30807
                     /locus_tag="KW011_001113"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBH7659513.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            30873..31121
                     /locus_tag="KW011_001114"
                     /pseudo
     CDS             30873..31121
                     /locus_tag="KW011_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            31186..31554
                     /locus_tag="KW011_001115"
     CDS             31186..31554
                     /locus_tag="KW011_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBH7659514.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            31648..32301
                     /gene="nfsB"
                     /locus_tag="KW011_001116"
     CDS             31648..32301
                     /gene="nfsB"
                     /locus_tag="KW011_001116"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HBH7659515.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            32409..33656
                     /gene="ybdG"
                     /locus_tag="KW011_001117"
     CDS             32409..33656
                     /gene="ybdG"
                     /locus_tag="KW011_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HBH7659516.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(33724..35100)
                     /gene="pheP"
                     /locus_tag="KW011_001118"
     CDS             complement(33724..35100)
                     /gene="pheP"
                     /locus_tag="KW011_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HBH7659517.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(35202..38345)
                     /gene="cusA"
                     /locus_tag="KW011_001119"
     CDS             complement(35202..38345)
                     /gene="cusA"
                     /locus_tag="KW011_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HBH7659518.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(38357..39580)
                     /gene="cusB"
                     /locus_tag="KW011_001120"
     CDS             complement(38357..39580)
                     /gene="cusB"
                     /locus_tag="KW011_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HBH7659519.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(39596..39928)
                     /gene="cusF"
                     /locus_tag="KW011_001121"
     CDS             complement(39596..39928)
                     /gene="cusF"
                     /locus_tag="KW011_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HBH7659520.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(39952..41334)
                     /gene="cusC"
                     /locus_tag="KW011_001122"
     CDS             complement(39952..41334)
                     /gene="cusC"
                     /locus_tag="KW011_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HBH7659521.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQSS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            41491..42174
                     /gene="cusR"
                     /locus_tag="KW011_001123"
     CDS             41491..42174
                     /gene="cusR"
                     /locus_tag="KW011_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HBH7659522.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            42164..43612
                     /gene="cusS"
                     /locus_tag="KW011_001124"
     CDS             42164..43612
                     /gene="cusS"
                     /locus_tag="KW011_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HBH7659523.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
     gene            44350..46252
                     /locus_tag="KW011_001125"
                     /pseudo
     CDS             44350..46252
                     /locus_tag="KW011_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243511.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
     gene            46280..46741
                     /locus_tag="KW011_001126"
     CDS             46280..46741
                     /locus_tag="KW011_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HBH7659524.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            46761..51172
                     /locus_tag="KW011_001127"
                     /pseudo
     CDS             46761..51172
                     /locus_tag="KW011_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR/RHS domain-containing protein"
BASE COUNT        12422 a        14007 c        13296 g        11553 t
ORIGIN      
        1 attatgggaa ataaataaaa ggtaattcat ggatggttga actatatccc tgcttttcaa
       61 ccatcctaaa aaagcatgaa ttgctcgctc taccctaagt ggctgtgcta ttttagaaaa
      121 acttaatcct gagttaagca aaaaaacata atcaataaaa atacatacgc aaaaaacatt
      181 gattaagtga atataccatg gaagaaaaat ataaaccgga gtagtgtatg gccaatctct
      241 acgacttgaa aaaattcgac ttaaatcttc ttgtcatatt tgagtgtatt taccagcatt
      301 taagcattag caaagctgct gaatcactat atatcacccc atcagccgtg agccagtcgt
      361 tacaacgctt acgtgcgcaa tttaatgacc ctgtattcat tcgctcagga aaaggtatcg
      421 cgccgacgac aacagggttg aatctgcatc accatcttga gaaaaacctc aaaggccttg
      481 aacaaacaat taatatagtc aataagtccg aactaaagaa aaactttatt atctacggcc
      541 ctcagctcat ttcttgctct aataatagta tgctgatccg ctgcctacgg caagatgcct
      601 ctgtcgagat tgagtgccat gatatcctca tgtcagcaga gaatgctgaa gaactgttgg
      661 ttcaccgcaa agccgatctg gtgatcaccc aaatgccagt gatcagccgt tccgtcattt
      721 gcatgccatt gcatacaatt cgcaatactc taatttgtag taataagcat ccgcgaatta
      781 ctgacaatag cacttatgaa caaattatgg cagaagagtt cacccaatta atatccaaat
      841 cagcaggagt tgatgatatt caaatggaaa tagacgagaa gtttatgaat cgcaaaattt
      901 catttcgtgg ttcctctttg ctgacaataa ttaatagcat tgcagttaca gatttactgg
      961 gaatagtccc ctatgaactt tataactccc accgtgactt tcttaattta aaagagataa
     1021 aactagaaca tccgttacct tcaataaagc tttacatttc ctataataaa tcttctttaa
     1081 ataatttggt gttctcccga tttattgacc gtctaaatga gagtttttga tatataacta
     1141 cagacagcta ctataacttt catctattta ttcacagcgc gaatgaaaaa taaaacactc
     1201 tgctttacag tgtttacttt ataattaaca gtcttatctc actgttcacc ctgtaacgtt
     1261 cagagagcgt cttcagatgt ctatttataa aattcctctt ccgctcaata ttctggaagc
     1321 tgcacgagag cgtattacct ggacactcaa caccctaccc cgcgtttgtg tctcattttc
     1381 tggcggcaaa aactctggtt taatgctgca tctgacagcc gaacttgccc gacaaatggg
     1441 caaaaaaatc tgcgttttgt ttatcgactg ggaggcgcaa ttctcttgca ccattaacta
     1501 tgttcagtcc ctgcgcgagt tgtacaccga tgtcatcgaa gagttttact gggttgcgct
     1561 cccgcttacg acgcaaaatt ccctttcaca ataccaaccc gaatggcagt gctgggaacc
     1621 tgatgtcgaa tgggtgcgtc agcccccgca agatgcgata accgatcctg actttttctg
     1681 cttttaccag ccaggcatga ccttcgaaca atttgtacgt gagtttgccg aatggttttc
     1741 acaaaaacgt ccggtggcga tgatgatcgg catccgtgcg gatgagtcct acaaccgttt
     1801 tgtcgccatc gccagtttaa ataaacaacg ttttgccgac gataaaccat ggactacggc
     1861 cgcaccaggc ggtcatagct ggtacattta ccccatttac gactggaaag tggctgatat
     1921 ctggacctgg tatgcaaatc atcaaaatct ctgcaaccca ctgtataacc taatgtatca
     1981 ggcaggcgtt cctctgcgtc atatgcgaat ttgcgaacct tttggcccgg agcaacgaca
     2041 aggattatgg ctctatcacg ttatcgaacc ggatcgctgg gctgctatgt gcgcacgagt
     2101 cagcggggta aaaagtggcg gcatttacgc cggacatgat aatcatttct atgggcatcg
     2161 gaaaatcctc aagccagaac atttagactg gcaagaatat gcattattgc tgctcaatag
     2221 catgccggaa aaaacagctg agcattaccg caataaaatc gccatttatt tgcactggta
     2281 tcagaaaaaa ggcatcgagg ttccacaaac ccagcaaggg gacattggcg cgaaagatat
     2341 cccctcctgg cggcggatat gcaaagtttt actcaataac gattactggt gtcgggcatt
     2401 atcatttagt cctacgaaag cgaagaacta tcagcgttat aacgaacgga taaaaggaaa
     2461 acgtcaggaa tgggggatac tatgcaacaa cgattaacgc aggatttaac gcaatttctc
     2521 gccagcttgc cggaagatga tcgcatcaaa gcgattaatg aaatccgcat ggcgatccat
     2581 caggttagcc cctttcgtga agagccagtg gattgcgttc tgtgggttaa aaacagccaa
     2641 cttatgccta atgattacaa cccaaacaac gtggcaccac ccgaaaaaaa actgctgcaa
     2701 aaatccattg aaatagatgg ttttacccaa cccattgtgg tcacacatac ggataaaaat
     2761 gccatggaaa tcgtggatgg ttttcaccgc catgaaattg gtaaaggctc atcatcatta
     2821 aaattacgct taaaaggtta tttgccggtg atctgtctgg aaggtacgcg taatcaacgc
     2881 atcgccgcga ctattcgtca taatcgcgct cgtggacgcc atcaaatcac cgccatgtcg
     2941 gaaatcgtcc gtgaacttag ccagttggga tgggacgata ataaaattgg caaagagctg
     3001 ggaatggaca gcgacgaagt gctgcgcctg aagcaaatta acggcctgca agagctgttt
     3061 gccgaccgtc aatattcccg cgcctggacg gttaaataac taaagctggc gcaggcgttc
     3121 agctgctgcc agcaacgtcg attccttctt ggcaaaacag agacgaatca gtttatgtgg
     3181 gaagggatcg gcgcaaaaca ccgacagcgg aatcgccgct acgccgtgct cctgcgtcag
     3241 ccactggcaa aactcaacat catccagggt agaaaccgcg ctgtaatcca ccagcaaaaa
     3301 gtatgtacct tcacacggta aaatctccag ccggctttca tttaaggcat ttaccagaat
     3361 atcgcgcttc tggcgataaa agtccggtaa cgcaagataa tgctcaggtt ctgcacgtag
     3421 catatcagca agcgccagct gtgccggggt attcaccgaa aaggtcagat actgatgtac
     3481 cttgcgaatt tcggcgctga tgggcgctgg cgcaacacaa taacccactt tccagccggt
     3541 catatgatag gtcttgccaa atgaagaaac cgccactgcc cgctcacgca gctgcgaatg
     3601 cgccagcaca ctggcatggc cctgttgtga aaagttgatg tgctcgtaga cttcatcgct
     3661 aatgacaaaa atctcgtgcc cggcgatcgc ctgccacaaa gcggcgaaat cagcctgctg
     3721 ccagacagtt gcactggagt tatgcggagt gttgaggatc accagtctgg tgcgctcgct
     3781 taataatgcg gcaaattcct gccagtcaac gcgaaaatgc ggtggttgca gtgccatacg
     3841 cttcactatt cccccagaaa gcgcgatggc gggggcgtaa ctgtcatagc tgggatcaaa
     3901 acaaatcact tcatcgccat tgcgcaccag tgcggtaatc gccgcgtata acgcttccgt
     3961 cgcccctgcc gttacggtga tatcgctatc ggcatctggt tgatagccat acaaacgttc
     4021 cgttttctga gcaatcgcct cgcgcaaggc ctgcacgccg gtcatgggcg cgtattggtt
     4081 tgccccctgg gcaacgtggt acgccagccg ctcctgtaaa tagcgcggac catcaaaatc
     4141 aggaaagcct tgcgacaggt taatcgcctg gtgttgctgc gccagcgcgc tcatctgggt
     4201 gaaaatagtg gtgccaagtt gtggaagttt gctttgtgga atcagagggt tatttgtcat
     4261 ttttattgta cctgacgaag ttgcgtgttg aaggcactat aacacgatgc tagtatttgg
     4321 caatcaagac gtttagatgt ctaaataaaa caataaggac aacacaacat gcctcacaat
     4381 cctatccgcg tggtcgtcgg cccggctaac tacttttcac atccaggaag tttcaatcac
     4441 ctgaacgatt ttttcactga tgaacaactt tctcgcgcgg tgtggatcta cggcaaacgc
     4501 gccattgctg cggcgcaaac caaacttccg ccagcgtttg gacttccagg ggcaaagcat
     4561 attttgtttc gtggtcattg cagcgaaagc gatgtacagc aactggcggc tgagtccggt
     4621 gacgatcgca gcgtggtgat tggcgtcggc ggcggcgcac tgctcgacac cgcgaaagcc
     4681 ctcgcccgcc gtctcggtct gccatttgtt gccgttccga cgatcgccgc tacctgcgcc
     4741 gcctggacac cgctctccgt ctggtataac gatgccggac aggcgctgca ttatgagatt
     4801 ttcgacgacg ccaattttat ggtgctggtg gaaccagaga ttatcctcaa cgcgccgcaa
     4861 caatatctgc tggcggggat cggtgacacg ctggcgaaat ggtatgaagc ggtggtgctg
     4921 gctccgcaac cagaaacgtt gccgctaacc gtgcgactgg ggatcaataa tgcgcaggcc
     4981 attcgcgacg tattgttaaa cagtagcgaa caggcgctga gcgatcagca aaatcaacag
     5041 ttaacgcaat cattttgcga tgtggtggat gctattattg ctggtggtgg gatggtcggt
     5101 ggtctgggcg atcgttttac gcgtgtggcg gcggctcatg ccgtgcataa cggtctgact
     5161 gtcctgccgc aaaccgagaa atttctccac ggcaccaaag tcgcttatgg cattctggta
     5221 caaagcgcct tgctaggtca ggatgatgtg ctggcgcaat taaccggggc gtatcagcgt
     5281 tttcatctgc cgaccacgct ggcggagctg gaaatggata tcaataatca tgcggagatc
     5341 gacaaagtga ttacccacac cctgcgtccg gtggaataca ttcattacct gccagtcacg
     5401 ctgacaccag atacgttgcg tgcagcgttc gaaaaagtgg aatcgtttaa agcctgacgt
     5461 gcaaatttag cagcagcgcg cgccgccttt tccaccgtag cgcgcgtcct gccgctcacg
     5521 gaaaaactct tcgtaggtca tcggcgtttg atcgggatgg ttaacccgca tatgttcgac
     5581 atagttgtcg taatcaggca taccaatcat cagcttcgcc gcctgaccta aatattttcc
     5641 ggcttttgcc agtgaatcaa acatcttggc ctccgctaac catgttgctg aaagagtgcc
     5701 ggatgcggcg tgaacgcctt atccggccta caaatgctat caacgcgctc ggtcagtccc
     5761 ctcgccccat cggggagagg gttagggtga ggaggaaccc tcggcactat gccaacattc
     5821 gccaacatcc cccctcactc tgactttagt gtgcgccttt tgcctgcgcc acgatctcct
     5881 cgacattttc cggcattggc tcatacggcg tttctttcgc cgttggcttc ggatctttca
     5941 atgccgccag tgccgactta atcgagaaca gcgccagaac caccacgacc accataaaga
     6001 agatggttaa cccggcatcc agacggttgt tgaacaccag ttgcgccagc tgtgactcag
     6061 tatactgcga cggaatattg ccgctgtcga tcattgcctg gaacttatta gcaatggcca
     6121 ggaagccgac tttcgcatcc gggctaaacg ctttctgcca gcctgcggtc agggtacaaa
     6181 tcagcagcca ggccgttggt accagcgcca cccaggcgta acgttgacgt ttcatcttga
     6241 acaacaccac ggcacagagc atcagcgcca tccctgccag catctggttg gcaataccaa
     6301 acagcggcca cagagtgtta atgccgccca gcggatcgac cacgccctga tggaggaagt
     6361 agccccacgc cagtacgcac agcgctgttg ccagcaggtt agcaggcagt gaatcggtcc
     6421 gcttcaggcc aggagacacc acgcccagca gatcctgcaa cataaagcgc gcagcacgcg
     6481 tacccgcatc taccgccgtc agaataaaca gagcttcaaa cagaatggcg aagtgatacc
     6541 agaacgccac atccatcata ccacccagcg caccgtgcag gatgtaagcc atccccaccg
     6601 ccagcgtcgg cgcaccgccc gcacgggaaa tgatcgactg ttcacccact tcgctggcaa
     6661 tctggttgag cgtatccgga gtaatcgcaa agccccagct actcaccacc tgcgcggcag
     6721 aagcgaccac atccgccgtc cctgccggag ccagcaccgc catcgggctg ttcatggcga
     6781 agtacacgcc cggatcgatg atacaggcgg aaaccagtgc cataatcgcc acgaaggatt
     6841 ccattaacat tccaccgtag ccgataaagc acgcctgccc ttcgttcgcc agcatcttcg
     6901 gcgtggtgcc ggaagagatc agcgcatgga agcctgacac cgcgccacag gcgatggtga
     6961 taaacaggaa cgggaacaga ttaccggtcc ataccgggcc agtgccatcg acaaatttgg
     7021 tcagcgcagg catggtcagc gtcggacgca taatcaaaat gcctaccgcc agaccaacga
     7081 tagtcccgat tttcaggaag gtagagaggt agtcacgcgg ggccagcagt aaccacaccg
     7141 gcagcaccgc cgccacaaaa ccgtagccca ccagcatcca ggtcagctgc acgccggtaa
     7201 agtcaaagta cggtgcccag gtcgggcttt ctgccaccca gccgccagag ataatggcaa
     7261 aaataaggaa caccaggcca atcactgaca cttcgccaat acgccccgga cgcagatagc
     7321 gcaggtagat tcccataaaa atcgccagcg gaatggtgaa cgcaacggta tacgttcccc
     7381 acgggctatg agtcagggct ttcaccacga tcatcgccag tactgcaagg ataatgacca
     7441 tgatcataaa gcaggctacc agcgcaatca ccccggcagt tggccccatc tcttctttga
     7501 ccagctcacc cagcgagcga ccgtcacggc gcgtagaaac aaacagcacc atgaaatcct
     7561 gcaccgcacc ggcgagaacc accccggcga gcagccagat catccccggc aggtagccca
     7621 tttgcgctgc cagcaccggc cccaccagcg gccctgctcc ggcaatggcc gcaaaatggt
     7681 gaccgaacag cactttcttg tccgtcggca catagtccag cccgtcgtta tggcgcaccg
     7741 ctggcgtcat acgcgtcggg tcaaccgcca gcacattttt ggcgatatac agcccataaa
     7801 aacggtaagc gatcagatag atacagaccg acgccaccac aatccacagc gcgttgatct
     7861 gttccccacg atttaaagca atgtatccca gagcaaatgc tcccattaca gagagcactg
     7921 tccagacgag gtatttccct gatttgttca tagttgttat ccgtgtgcgt gtccatagag
     7981 atgttacatt ttgtttctat aacatctttg tacgatttaa caacacggta accatgtaaa
     8041 tcggagttga aaccaatatt taacttaaca atgttaactc gatcactccg ttaaccgagt
     8101 tttcctgcaa tctcatttaa ttctgtcggg ctcatcccaa aactgccgta cccagccgac
     8161 aagtgcagca acgccgcccc tgttcatcga aaacgatgat ttcccagctc tgattttgcc
     8221 gcccaagatg cagcggctgg cagacgccgc gtaccttgcc ttcagacacc gggcgatgat
     8281 gggtggcgtt aagttccgta cccaccacac actgcccgtc acgggtcatc ataaatccgg
     8341 ccatcgatcc cagcgtttca gccagcgccg ccgacgcgcc gccatgcagc aggccaaacg
     8401 gctgatgagt acgggtatca accggcattt cggcttccag cacatcatct cccagacggg
     8461 tatacacaat tcccagatgc gccaccattg tgttatcgct ggtggcgttc agttcgtcga
     8521 gcgttaaatg gcgtttccag atcatgctta tgcccccagc gttgagccgc catcgaccac
     8581 aatatcctgt agggtgatat ggctggcgag gtcagaggcg aggaacaaaa tcgtgttggc
     8641 gatctcttgt ggacgggcga ttttccccag cggaatgccg agtttaaact gctcgccaaa
     8701 gccgcgaata cgctgttctt cggcgtcatc gctcacccac agcgtgcgtt gcatatcggt
     8761 gtcggtggag ccaggcgaaa ccacattaca gcgcacgccg ctaaccgcca gttccagccc
     8821 gacgctcaac gccaggcttt tcagcgccgc tttcgatgcg ccataagcac tcataccaat
     8881 acgcggcgtg tgtgcggcgt cggacgccac agtgacaatc gccccgcccc gctgacggcg
     8941 aaactggttc atggtttgct gaaacaggtt aaacgcaccg ccgacgttaa ccgcaaaagt
     9001 ctgctgccag tcctctttac tgagctgatc ggtcgcgccc atgcgcaaaa ttcccgccgc
     9061 attgaccagc acgtccagtc gctccgtttc agctaacagt cgctgacata cctgcgcgac
     9121 ctgcgcagca tcggcaacat ccatcacttc ggtcgcaaag ggatattgct cctgagcgaa
     9181 cgcttgatca aaaccggtaa ctttcgctcc cgcttcaaca aacgccagcg ccgtggcgta
     9241 gccgatacct ttacctgcgc cggttaccca gacattttta ccgctgaaat ccattatttc
     9301 acctcgcggg agagtagctt ccaccaggcg tcgatggtcg ggtttttcgc cagcatgaca
     9361 aagtcgatat cgccatgcac tttgcgccag cgcgccgcca gcgccatcat gcgcaccgaa
     9421 tccagaccgt agtcgatcag gttgtcgtca tcgaacggtt catcggactc gtccagcaac
     9481 ggcaggataa cctcacgcag cgccgctttc ctggcgggga caggtgctgg cagtaatcct
     9541 tcagtcatca ccacccggcc agaccgcccg gccacatatt tcagcgacat caaatgctcg
     9601 tcacggctga aatcggccag cgcatccgcc accataaacg gtttaatatc gcgcataaat
     9661 gcgtcggttg cggtggtcat acagccaatg tgggcatata ccccggtaat aatcagctgg
     9721 ttacgtccac tctctttgag catttgctcc agcggagaac gatgaaacgc gctgtagcgc
     9781 cacttcacca gcaccgtgtc gtcggcatct ggcgtcaggc gatccaccac cttttgctgt
     9841 tccggcgaac gggtcaggcc cggcccccac atatcattca acagcgcccg atcttcatcg
     9901 ctctgctctt tcggctgggc ggtgtaataa accgggatat tgtgctgttt gcagtagtcg
     9961 cgcagcgcag caatattcgc gatcacctgc tccatcatcg ggcagttctc gccccagaag
    10021 ctgacaaaat agtcctgcat atcatggatt aacaacgcgg cacgttgcgg ttcaaaggcc
    10081 cagtcaactt tattctgcgg aatatcgtga gactccggca gtgcgtaagc ctgtaatttt
    10141 ggaatagcca tcgtgttctc tccttcaggc tgatgcgcgt gacgccagcc actgacgtaa
    10201 ttgtttttta tcgactttcc cgaccgccgt cagcggaagt gaatccacac actccacgcg
    10261 atccggtaat ttaaattcgg caataccctg ttcacgcagg aaacgacgca cctgcaccgc
    10321 gcgcagcggc tcttttacca ccagataagc acagcttttt tcgcccatca gctcatcttc
    10381 catgctcacc agtgcggcat agatcaccgc cggatggcgc agcagcaggt tttcgatctc
    10441 ttcggcagcg atcttctcgc cgccacggtt gatctgatct ttctcgcgcc cctgcacggt
    10501 gatgtaaccc tctggatcaa tagagatcag atcgccggaa cagtaaaaac cgttggcatc
    10561 aaaggcactg gcattgtgct gcggactttt gtaataaccg cggaaggtgt acggcccgcg
    10621 cgtcatcagg cgtccaactt ccccttgcgg cagtggattt ccttcagcat cagcaaccca
    10681 tacttcatcg tccggacaca ttgggtaacc ctgggtatgg ataattttct ccgcgctatc
    10741 atcaagtcgg gtgtagttca ccagcccttc cgccatgcca aacacctgct gcaactggca
    10801 gccaatctca gcggaaatac gcgccgcaag ggtggcagaa agacgtgcgc cgccgacctg
    10861 taacagtttc agcgaggcaa gctgcgcccg gctttcgcct tcagccagcg cctgcaacca
    10921 caggctgact gccggcggca ccagcgcggt aacgttaacc tgatgttttg caatcaacgg
    10981 gaagcaaagc gtggcgctgg gatcggccgc cagaacaacc gttcctccgg caagaaagac
    11041 gcccagcgat cctggcgaac tcatagcgta gttatgagcc gccgggatcg cgcacaggta
    11101 gcgtgtctgt tgtgtgaact gacaaatctc gacgctacga cgcacgctgt agtagtagtc
    11161 gttatgagtg cgcgggatca gcttcggtgt accggtggtg ccgccggaaa gctggaaata
    11221 ggccacttca tcagcaggtg atggcgtagc agtaaaaccg tctgccggat ggttaatcgc
    11281 atcctgcaag ttatgctcac cgctgtcgtt gagcagttgc accacgcgaa tggaggaatg
    11341 ttctgtgacg aaagtattga ggaaatcatc cccgctaaac agcgcatgtt ggcgatcggc
    11401 aatcagtaac gccggttcaa tctggctggc ataggcgttc agttcactac gctgatggct
    11461 gaacaacgcc agcaccggcg caacgcccag tttcagcagt gcgaaaaagg taatgtaaag
    11521 ttcagcgacg ttacccagtt gtaccagcgc ggtttcaccg ggtttaatgc cctgacggcg
    11581 taaactacac gcgaggttat ccgccgcctg attcagctcc cggtaactca actgtcgctc
    11641 gccgtcgata accgcgatgc tgtcactcgc agcgtggcga gtcagaatgt cggtcagcgg
    11701 caaatcctgc cagtagcctt tttcccgata gcgacgggca aactcttccg gccagcgggt
    11761 gaatggaatg ctcatcctcg ctccttaatg caatccaaaa acgttcaaca tggtagaaag
    11821 tttgacgcct gtttcgcgcc actcacccaa cggtgacgac gcaggcacaa tccccgctcc
    11881 ggcaaacaga cgcacctgat tttcccgcag cttcgcgcag cggatggtca ccacccattc
    11941 gccgttacct tcgctgtcac accaacccac aatgccgcca aacagttcgc ggtcaaacgg
    12001 ctccagttca gcaataacct gggtcgcggc ctgatgcggg aaaccgctca gcgcgggggt
    12061 cggatgcagc agacaggcca gagtcagtgc gttttcttgc gaattcgctt taccttcaaa
    12121 gggagttgcg agatgccaca gcgtcggcgt ggtaatcaat tgtggagagg agggaacgtg
    12181 taactcacta ctgcgttcgc gcagtacctc tttcatcgcc tgagtcacca gttcatgttc
    12241 atggcgatct ttttctgacg ccagcagacg attacccgct tcgcgatcga gcacttcatc
    12301 cggctgacga cgcgcggaac cggctaacgg aatggagcta aaacgctcgc cgtctttacg
    12361 tagcagcagt tccgggctgg cccccagcag gacgccacca tcagccagcg ggacatggaa
    12421 gttgtaacta accgggtttt gcgcaatcaa ccgttccagc aatacgccac tatcaatggc
    12481 ggcgtcagtg gtgatatcaa tcaatcgtga caacaccact ttgtcgacct gcggcgtggc
    12541 ggtaagtgcg gcggcgcggg caaccatctg ttcaaacgtg gtttgttcag gaattgcctg
    12601 gcgttccacc acgttcagcg actggctgcg ggtaaaacag tgggctgagg tctgtttttc
    12661 ctgacgggag aacgactgcc aggattcggg aatatacagc gacgaaggct gacgtggatc
    12721 gaagggaatc gccccgacca tcaccggatt tttgatgccc tgcgctttgg catcggcaaa
    12781 cagcgcggcg agtttttgct ggaagggact gtcgggcgaa tccccgttca cagccggttc
    12841 atcgaagcgg gcgaaacatc ctgacgtcgt aaaactgcgg tacggcgaca taaagaaaaa
    12901 gcgattgggc gcaagtgttg ccatggtctg ctgtacttcc tcagccagtg acgtatccat
    12961 atcatcctcc acaaaatgat aaaggcttta ataatgatta tcatttatat tttcggtcgc
    13021 taacctaaaa gcaaacgcgc actatgtcaa ctcttgaggt aacgcaatct gcaactcggg
    13081 gttgcatctg gttgtggtta caatgaaaat gagaagcatt attgatgaat tcgcataagc
    13141 gcaatgtgat gtcctgcgcc gttttgcccc ctctcccttc cagggtgagg gctggggtga
    13201 gggttaatgt tcgcaccagt gctggctgtt cccctcaccc taaccctctc cccaaagggg
    13261 cgaggggacg gattgtgcgc tttgtcgaat ttgtcattac gcccttaacc ttattaataa
    13321 caggaagctg acttgtgaga ctcgccccgc tctaccgcaa cgcccttcta ttaacaggac
    13381 ttttgctttc aggaatagcc gtagttcagg ccgccgactg gccgcgtcag attactgaca
    13441 gccggggcac tcataccctg gaaagccagc cgcagcgtat tgtttccacc agcgtcaccc
    13501 tgaccggatc actgctggcg attgatgctc cggtgatcgc cagcggcgcg accacgccga
    13561 ataaccgcgt cgcagatgac cagggctttt tacgccagtg gagcaaggtg gcgaaagaac
    13621 gcaaactgca acggctctat atcggcgaac cgagcgccga agccgttgcc gcgcaaatgc
    13681 cggatctgat tttaattagc gcaaccggcg gggattcggt gctggcgctg tatgatcagc
    13741 tttccaccat cgccccgaca ttaatcatca attacgacga caaaagctgg cagtcgctgt
    13801 taacgcagct tggcgaaatt accgggcatg agaaacaagc ggcagagcgg attgcacagt
    13861 ttgataagca actggcagcg gcgaaagagc aaatcaaatt accgccgcag ccggtcactg
    13921 ccattgtcta taccgccgct gcacacagtg ccaatctctg gacgccggaa tcagcacaag
    13981 ggcagatgct ggaacaactc ggctttacgc tggcgaagtt gcccgcaggg ttaaacgcca
    14041 gccaaagcca gggcaaacgc catgacatca ttcagcttgg tggggaaaat ctggctgctg
    14101 ggttaaacgg tgaatcgcta ttcctgttcg ccggtgatca gaaagacgcc gatgcgattt
    14161 atgccaatcc actgctcgcg cacctgcctg cagtacaaaa caagcaggtt tatacgctgg
    14221 gaaccgagac gttccgcctg gattactaca gcgccatgca agtgctggac agacttaatt
    14281 cactgttaaa aaaagattga taaaacatac aattagagca ctaataatcc cctcgcccct
    14341 ttggggagag ggttagggtg aggggaaaat gcacctttag ctaactgtca gacgctgtca
    14401 cctgcggcgg cgtctggcga aaacgtcgca actccaccag caccagcaat aacaacacgc
    14461 cgataatcaa caaaccaaaa ccgctcgcgc ttgcggaagc aaccggtgtc atcatcgcgc
    14521 ccaaaccacc caacagcgcc gcgcctatgg catcgcccgt cacgttctgc gccgtccaca
    14581 aaccgttaat ccgccctaac atcgcttccg gggtttgcgt ttgcagcatt gtatattgca
    14641 gcaacgaact aaccgcgctc agccagccga acagcgccag acaaaccacg cctaaaatcc
    14701 acatcggcat caggccaaac agaccaatgg cgaggaacga tcccagcgtg gagagcagca
    14761 tcaataaccc tggtcgcgca ctatgtgcca gcttcccgct ggttaacgca ccaatagccg
    14821 caccgagcgg gatcgccgca tagagaaaac caatctgtgc cgccgacatc tgccagttgt
    14881 cagccagcgc cggatacagt acccgcaccg cgctcgccat cgtcaataaa ccacccagca
    14941 acgcaatccc tcctaccagc gggctggcga gcagaaaacg aaatcctgcc agtaatgatt
    15001 tcaacggatg ctcgcgcggc tgcggtggcg gtggcaacgc cggaaggctt aacaacggta
    15061 gcaaggtaat aaacgtgccc gccgccgcca gcccgtagtt ccaggctacg ccgccggtcg
    15121 ccagcaataa accgccaatc atgggcgaaa tcaccgaccc cagacgcacg gtcaacatgg
    15181 tgatcgcccc ggcctgcatt aagttttcac gccctaccag tgcaggtgtt gccgccagta
    15241 gcgccgtaac accaagtgat gcgaaaaaac catcccataa accaagtaaa tagattgcca
    15301 gcaatgacgg ctccggcagc agcgcgttca ggcacagtcc aatgaagcca atgccacagg
    15361 tgccgcgcgc cagcaaaatc acttttttgc gctcatagcg atccgccagc acaccgccaa
    15421 ccatcaggcc aacaaacatc gcgccgccgg tcagcgtcac cgaaagcccc acctgccagg
    15481 tagaatgcgt catcatctgg atctgcaccg gcaccgcgac gccgagcaaa cccagagaca
    15541 caattgagat aaaacgagcg aggaatactg cgcgaaacgc cgggtgcgtt ttcaacaggc
    15601 tgaggttaag cagccaggat tgtttattca ttacaatgcc ttgccatcaa atattttaaa
    15661 tacctattct taggctgcac atgctaacat atccaaataa gatcgataac gataattaat
    15721 ttcattatca tggaagttcg tatgtctggt tctgttgccg tgacacgcgc cattgccgtg
    15781 cccggattgc tgttattact gattatcgcg acggcattaa gcctgctcat tggggcaaaa
    15841 tcactccccg cgtcagtagt gctggaggcc ttctcaggca cctgccagag cgccgactgc
    15901 accatcgtgc tcgacgcgcg actgccgcgc acccttgccg gtttacttgc aggcggtgcg
    15961 cttgggcttg ctggggcgtt aatgcaaacc ctcacccgaa atccacttgc cgaccccggc
    16021 ttgcttggcg tgaacgccgg agccagcttt gccattgtgc tgggtgcggc gctgtttggt
    16081 tactcatccg cgcaggaaca actggcgatg gccttcgccg gggcgctggt ggcctcattg
    16141 attgttgcct ttatcggcag tcagggcggc gggcagttaa gtccggtgcg tttaaccctg
    16201 gcgggcgtgg cgctggcggc ggtgctggaa gggctgacca gcggcatcgc cctgcttaat
    16261 cctgacgtct acgatcaact gcgtttctgg caagccggtt cgctggatat tcgcaatcta
    16321 cataccttaa aagtggtgct gatcccggtt ctgatcgccg gagcaactgc gctattactg
    16381 agtcgcgcgc tgaacagttt gagcctcggc agtgacaccg cgacggcgct gggcagtcgc
    16441 gtggcgcgca cacagttgat tggtctgctg gcgattaccg tgctttgtgg tagtgcgacg
    16501 gcggtagttg gcccgattgc ctttatcggc ctgatgatgc cgcacatggc gcgctggctg
    16561 gtaggtgccg atcatcgctg gtcgctgccc gtcacgctac ttgctacccc tgccctgctg
    16621 ctgtttgccg atatcatcgg gcgggtgatt gtgcccggcg aactgcgcgt ttctgtggtc
    16681 agtgcgttta ttggtgcacc ggtgctgatc ttcctcgtgc gacgtaaaac gcgaggtggt
    16741 gcatgattta cgtctctcgc cgattactca tcacctgttt gctgctgatt tccgcctgtg
    16801 tggttgcagg tatctgggga ttacgcagcg gtgccgtcac gctggaaatc tcgcaggtat
    16861 tcgccgcgtt gatgggcgat gcgccgcgca gtatgacgat ggtggtcacc gaatggcgtt
    16921 taccacgcgt gctgatggcg ctgttgattg gcgcggcgct gggcgtcagc ggcgcgattt
    16981 ttcagtcgct gatgcgtaac ccgctcggca gccctgacgt aatgggcttt aacaccgggg
    17041 cgtggagcgg cgtgctggtg gcgatggtgc tgtttggtca ggacctgacg gctatcgcgc
    17101 tggcagcaat ggtgggcggc attgtcactt ctctgttggt ctggctgctc gcctggcgta
    17161 acggtatcga cacctttcgg ttgattatta tcggcatcgg cgttcgcgcc atgctggtgg
    17221 cctttaatac ttggctgttg ctgaaagcgt ctttagaaac ggcgctaaca gcgggtttgt
    17281 ggaatgccgg atcgctcaat ggcctgacgt gggcaaaaac ttcgccttcc gcacccatca
    17341 tcatattgat gctcattgcc gccgccttgc tggtgcggcg aatgcgcttg ctggagatgg
    17401 gcgatgatac cgcctgcgcg ctgggcgtca gcgtcgagcg ttcgcgtctg ttaatgatgc
    17461 tggttgcagt ggtgcttacc gctgcggcaa cggcgcttgc cgggccgatt tcctttattg
    17521 ctttagtcgc gccgcacatt gcccgacgca ttagcggcac cgctcgctgg gggctgaccc
    17581 aggcggcgct gtgcggagcg ctgttactgc tggcggccga tctctgcgcc caacaactgt
    17641 ttatgccgta tcaacttccg gttggcgtcg ttaccgtcag cctcggcggt atttacctta
    17701 tcgtcttgtt aattcaggag tctcgcaaaa aatgaccgaa tcagtagccc gtttgcgcgg
    17761 cgaacagtta accctgggat atggcaaata taccgttgcg gaaaatctga ctgtagaaat
    17821 tcctgatggt cacttcacgg caattatcgg accaaatggc tgtggtaaat ccacgttact
    17881 gcgtacctta agccgcctga tgacgcctgc tcacgggcat gtctggctgg atggcgagca
    17941 cattcaacat tacgccagta aagaagttgc acgccggatt ggtctgttgg cgcaaaatgc
    18001 taccacgccg ggcgatatca ccgtgcagga gctggtggcg cgtggacgtt atccgcatca
    18061 accgctgttt acccgctggc gcaaagagga tgaagaagcg gtaacgaaag cgatgcaggc
    18121 cacgagaata actcatctgg cagatcaaag cgtggatacc ctttctggcg gacaacgcca
    18181 gcgagcgtgg atcgcgatgg tgctagccca ggaaacggca attatgctgc tcgacgaacc
    18241 cacgacctgg ctggatatca gtcatcagat tgatctactg gaactgttaa gcgaactgaa
    18301 ccgcgagaaa ggctataccc tggcggcggt gctgcacgat cttaatcagg cctgtcgtta
    18361 cgccagccat ttgattgcat tgcgggaagg gaaaattgtt gctcagggag cgccgaagga
    18421 gattgtcact gctgaactga ttgagcgcat ttatggtctg cgatgcatga tcattgacga
    18481 tccggtagcc ggaacgccgc ttgtggtgcc gctcggacga acggcacctt caaccgcaaa
    18541 tagttaaact aagtggtctg ccatcatggc atcctgtttt ctggatgcca tcgcatagcg
    18601 caataacacg ctaccacaag ccaccatccc gccgatcagc gcggaaagga tcacaataat
    18661 cgccttaccc ggaccgtctt ttttcactgg caatgacggg cttaactgat atttaaacgg
    18721 cgtaaaattc acatcgttga catttgcttt tgttaattgc tcgacaagat actgccgatt
    18781 acgtaattca ccgttcagtt ccgcaacgtc agtaaccgct ttttcaattt ccagtttgcg
    18841 ttcaataccg tctgcgccga gagaaataga gaaatcgggg tcatctttaa cggcctgacc
    18901 attactgtat acgggctttt taattcctgc cgcgttggca atgtcgagtg aataattgag
    18961 gcgctgaatg tttgcatcaa gttgattttt cgttttaatg cgatcctgag ccagtttttc
    19021 tttttcaaac tgggttttga tctccagttt attacggacg ttttctatcg actctttcac
    19081 caccaacgca gagatataat cgatataccc gctcaaaacg gtctgcgcct ctttactggt
    19141 tggcgcggta aaacttagcg tccaggaggt atacagtgac ggttcatctt ttttcttact
    19201 ggcattgtca tcaaccgctt tcattttttc gctcaatgca acaatagcgc gatgcaaatc
    19261 cagttcatcg attttcgcct cttttaattg gtccatcaca taaggtgatg aacgcaggta
    19321 ctcttccagc aagctaacag actgaaactt cttgataaac aggttaaatg caccgccacg
    19381 gtcgatatta acatcgagat caagaacgcg gagtttggta aaggttttct ccaggtcctg
    19441 ccactgaatg gcttcggcgg gagtaataac ggctgagctg gtccattttt gcggcaggat
    19501 gaaagagatc agtaaacctg cgcaggcaaa tgcaaaaacg accgccatga ccgttttttt
    19561 ggcccgccat aaaacttcga ttagattaag taaatcaatt tcattattac tgggcgacgc
    19621 cagaggatac tcagaaaaat gagcgtcact tccctgttta atattcaatg atgacatggg
    19681 gtataacctg aaaatgagtc caatagggga aaatctccac ggcgctcact ttaccagttg
    19741 gtgagaatta tccgaagctg aagtttgtaa attccttcca ctgaatgcaa tattcactct
    19801 atttaagaga aatattcatc aaacctataa atcaaacaaa taaaaaaccc ataattattt
    19861 tctgtaatta tgggtttata gataatatta atttacctgt ttagcgttgc gcgaataatc
    19921 ggcccaattt ttacaaacgc ccctggagag ataatatcca catgcgcaca atcctgacga
    19981 tagatatcca gctccgcaat ccacggcgac caggcgcgtt caggactcat accttcctga
    20041 agcgtgcgtt cagcaacaaa caacgtcgct ttaccgtcaa acggtacgct atgagcagtc
    20101 gtcagcaggc gcacagcatc agcgtagttg ccttcaatgg tggtaaacaa ctccgttgaa
    20161 gtacttccct gctgtgccgc caggaaggcc tcgcgctcgc ggttaatctc cgccagcact
    20221 tccgggtcca gaccattagc ttctttttcc tgccagtttt gcgtttctgg cggccaggta
    20281 tccagcaagc caagaaatgc cacctgttcg ccacgggcac gcagtcgcgc cgcaataccc
    20341 tgcgccagcg taccgccaag ggaatacccc agcaggtaat aagggccgtg cggttgttgc
    20401 tcaagtaacg ttgccagatg cgcttcgcag acttcatcca ggtttgtcgc tgtctgcatg
    20461 ggtccatgag ggcgcggcga ctgaatgcca ataatcgacc attgtggatc gagataacgc
    20521 gagagtacgc taaactgcca ggcaaaaccg gacgccggat ggaaacaaaa cagcgtcggg
    20581 ccattacctt cacgcaacgg cagaatggtt tcgaatccca tgcgccggga gctgtcctct
    20641 tcaccatcaa taatcgttgc cagtttggcg acggttgacg cgaccatcac ctgccccggc
    20701 gtcacctgac gagcaaactg tcgacttaac tgcgctgcca gtttcattgc cagtagcgaa
    20761 tgaccgccca gcgcaaagaa atcagcatcg gcatcctgca cgtcacaacc cagcaacgac
    20821 gcgaatgccg cggcgataat cgtttcactg cccgctttcg gtgcacgtcc cgacgcttgt
    20881 gtcttcagtt caggcaacgg taaggctttg cgatccagct tgccgttggc gctaagaggt
    20941 aactgtggaa gttgcagcag aaccaccggt accatatgtg gtggcaatgt ttcacgaagc
    21001 tgcgcctgca atgcgctggt atccaacggc aggcccgatt gcgacaccag atagcccacc
    21061 aactgacgcg catcaccacc agtggctgcc gcctggttaa tcacacaggc gtgggtaacg
    21121 gcttgttcga catccggcag cgcctgcatc acgcgatcga tttcgcccag ttcgatacgc
    21181 tgcccgcgaa tttttagctg atcgtcactg cgcccgaggt actccaccgc gccgttatcc
    21241 agccagcggg caacgtctcc ggtacggtac atccgttcgc caggagcaaa aggatcggca
    21301 ataaagcggc tggcggtcag atcggggcgt ccgagatagc cctgcgccag ttgaatgccg
    21361 gtaagataga gatcacccgc cacacccggc ggcaccggat gcatcatcgc atcaagaata
    21421 cgcagcccca tgttccacac cggataacca atcggcacac tgctgccgcg cacctgtgcc
    21481 agttcctcgc caaaagccgg ataccagctg acatctaccg ccgcttccgt cgggccatac
    21541 agattatgta acggcgcacc ggttaactgt tgccattcac ggcataaatc agccggtaag
    21601 gcttcaccac tacagaaaac ctgtttcaac gtcgcgcaac tctggcgagc ggtttgcgga
    21661 gtcagcgagg caacaaatgc cgccagcatc gacggcacaa agtgcgtggt cgttacgcca
    21721 tattcggcaa agaattgctg catagcgagc gggtcacggt gcgcttccgg ttcagccatc
    21781 accagttttg cccctgcgat aaacggccag aaaaactccc acaccgagac atcaaaactg
    21841 cacggcgttt tttgggcaac gacatcttcg cctgtaagcg ggtagtgatt ttgcatccat
    21901 aacaggcggt taacgatagc cgtctgcccg accattaccc ctttcggcct gccggtggag
    21961 ccagaggtaa agatgatata agccgtgtgg tgcggttgtg aaagttgcag cggcgcactg
    22021 ccctgggggg taagcggggc gttatagcaa aggcttgtta aattgggaac atcgctaaag
    22081 cgcggcagtt gatcgtcggt ggtaattaac agcgacggac gcgcatcttc cagcatcatt
    22141 ttcaggcgat cgtccggata gccggtatcc agcggtagcc aggccgcgcc agcttcaact
    22201 attgcatgga gtgccagggt caaaaagacc gagcgcggta gtgccaccgc cacgctgtcc
    22261 cctggtttaa cgccgcgctc acgcagcaga ttcgccagcg ccaccacctg ctcgcgcatt
    22321 tcccgatagc tgaacaggta acgcgcatct gccagcgccg gagcatccgg tgtttttgcc
    22381 gcttgttctg ccaccagcgc gctcagcgtg gtttctggaa tctcaacctg agtggcgttg
    22441 atctgcgcca gctgcgcata ctcacctggc agcataatat cgacatcgcc gcacaacagc
    22501 gccggatccg cggcgaactg ggcaatcagc attttcaggc gttcagcatg ctggattaac
    22561 gttggctcat cgtaacgctg tttattggcg aggatctcaa tactcaaatc accgtgttca
    22621 tccgggaaca gggccagttc aaggtcatta accggaccgg ttgccagggt atgggtttgc
    22681 gcctgaacac caggaatatc cagttggtaa tcaaatacct taatattgag taccggacca
    22741 aacagcggtt catcacctgc cgctcgcccg ctgtcacgga caatttgttc ggcatcgtaa
    22801 cgttgatggc gacgcatttt tttcagttga gccgccagtc gggttgccag ttccggcagc
    22861 gtttcttgcg ccgcaatgtg aatacccaac ggtaaaacgt tgagcacggg tccgctagcc
    22921 gtcagcgccg ccgagcccag tcgacgcata aagataaatc cggcggcata gtccatgcga
    22981 ttgcacaatc gccccagcca caaggctgcc agcgcaaggg ctaaatcggt acgctgcaca
    23041 cctgaaagtt gcgtagccag ctggcggaat tccccgtcgg taaattccag tttcaggcgc
    23101 agaatatctg ccgaagcgct gcgccccggt aaaggtgccg gagaaagtga cgcgggcggc
    23161 ggcagttgac gacgctgttc tgcccagaat gccgcatcac gctgccaggc ttcgctttcg
    23221 cggtattgct ggtactcttc cactacatca gcgaaaggcg taaacggcga agcaggcgtt
    23281 ggttcgccac gcagccatgt gcagtaaata ttggcgatct ggcgagtaat ggccgggaaa
    23341 ctgaagccat cgaccagcaa atggtgataa cgctgatacc agtaccagcg gttatccgca
    23401 acatgtatca gctgatgaaa gaccagtggt ttaccgctat cgacgcgcaa atcttgttgc
    23461 aaatccgtca gcattaatgc ctgcgcagta ccgtgcggat caatattggt tcgcaggtcg
    23521 ataatttctg gctgttcgaa tatcagcgca tcatcgaccc actgccagac ttcgccgtta
    23581 tcttccgtaa aacgcatccg caacgtatct gcttgcgcta gtccggcaac caccgcgcgg
    23641 gccagtaatg gcgcatcaac atctccagtt aactcaacgt aatgcgccac gctccaggcg
    23701 gagggtaaat ccgacagttt ttctgccatc cagatgccgg gctgtgcggc gaccaaaggt
    23761 aaatgctggc tcattgtgcc tcctgcaact gggtgaaatt ggccggtgtc agagtacgcc
    23821 agtggacttc cagccactgc tggcaggacg cctgtgactg cggctgacac acaatgtccc
    23881 agcctgccga taagaggcat tgttgcggcc acagactgaa ttgcccctgc gcattgcgca
    23941 aaatgtaaaa cgctccctgc ggatcatcga agggattact gaatgccata ctcaactcct
    24001 gtcatggaaa agtggttgcc agaggtcgat taacccctgc atcaagccac cgcgccaaca
    24061 aagcgcatca tgtccgccgt caacctgacg ccagaaaatg gattctttta tagggtgcaa
    24121 ttgcgcatac agcgcctgat tggcccgcat gatcatcggc tcgcgaatac ccgcttccag
    24181 cacaatgcgc agaccttcgg cgctaacttc accagctttt agcttttcaa gtaacacgcc
    24241 ctcttgctgc ccgtcccgat gcggccacca gtacgatcct gactggctta atacacagcc
    24301 aaagcgttca ggccagtgca gtccggcata cagcgcggac agcccaccaa aactctgccc
    24361 ggcgaccacg gtgcgatcgg cgcgatcgct aaaaggggca atagctttca ccaggggtaa
    24421 taactcttgc tgtactgcca gccagaaatc cgcattacac ggcagttcgt gggcgcggtg
    24481 cgtggtgtcg atagcgtcga tcaacacata cacggcggga ggaagttgct gacgatgggt
    24541 cagcgaagtc agcacaggcc agacgggcat actttgcgcc caaaattcgc catcgagcaa
    24601 aactgccagc ggacgttctt ctgctgttac atcgccggtg gtaaaaatcc atacacgacg
    24661 tgaatttttc aaccgttcac ttttccagat aatttctttg gctgctattt ctggcacttg
    24721 cggacaatcc catcccggtt gcagaggcgc ttgcggcatt tcgagtgcag aaacagcgtg
    24781 ccctcgcccg cctttccagc tttgtggatt cagcggatcg gctattgcct ggggtaatag
    24841 ttttcgccag ccttcacgta attcgaggcg atcgggggat ggtgcagaaa aaatgtcatc
    24901 gcgttcggtg ggaataaagc agtagctgcc gcgccagttg gcattgagtt gtgtcgtcca
    24961 ctgccagaca tcagtacctg caattcgctg catcgactgg ggctggctgt tctgatgatg
    25021 atcggtcaca ccagtgatgt agatccatac gcgctttatc gtcgagtatt cttcagaacc
    25081 ttggggatca cgccaccaga aagtgacctc aaacatttcg tcattcagac gctgccattc
    25141 cgggccatgt ttcgactgcc accagctctc acttcctact tttaacgccg tcacaccata
    25201 accccatgtt tactgtgcaa tttttcattg attgcagaaa tatattgata atattattga
    25261 taaccatttg catttgcaat agcgtaatgg cgcgccgtgg gaagcgcgga catttattaa
    25321 ccaactgcac tgcgtgtctt tcaggatcaa aggttttcgc ggtagcggga tgcgtcgtgt
    25381 tgaggacgac catgcccgac agttgcaatt cgtggcaaaa atgcaggaat aaaacaatga
    25441 acaagaagat tcattccctg gccttgttgg tcaatctggg gatttatggg gtagcgcagg
    25501 cacaagagcc gaccgatact cctgtttcac atgacgatac cattgtcgtt accgccgccg
    25561 agcagaactt acaggcgcct ggcgtttcga ccatcaccgc agatgaaatc cgcaaaaacc
    25621 ccgttgcccg cgatgtgtcg gagatcatcc gtaccatgcc aggcgttaac ctgaccggta
    25681 actccaccag tggtcagcgt gggaataacc gacagattga tattcgcggt atgggtccgg
    25741 aaaacacgct gattttgatt gacggcaagc cggtaagcag ccgtaactcg gtgcgtcagg
    25801 gctggcgtgg cgagcgcgat acccgtggtg atacctcctg ggtgccacct gaaatgattg
    25861 aacgtattga agttctgcgt ggtccggcag ctgcgcgtta tggcaacggc gcggcgggcg
    25921 gcgtggttaa catcattacc aaaaaaggca gcggtgagtg gcacggctcc tgggacgctt
    25981 atttcaatgc accagaacat aaagaggaag gtgccactaa acgcaccaac ttcagcctga
    26041 ccggtccgct gggcgacgaa ttcagcttcc gcttgtatgg caacctcgac aaaacccagg
    26101 ctgacgcgtg ggatatcaac cagggccatc agtccgcgcg tgccggaacg tatgccacga
    26161 cgttaccagc cgggcgcgaa ggggtgatca acaaagatat taatggcgtg gtgcgctggg
    26221 atttcgcgcc attgcaatcg ctggaactgg aagcaggtta cagccgccag ggcaacctgt
    26281 atgcgggcga cacccagaac accaactccg attcctatac ccgctcgaaa tatggcgatg
    26341 aaaccaaccg tctgtatcgc cagaactact cgctgacctg gaacggcggc tgggataacg
    26401 gagtgaccac cagcaactgg gtgcagtatg aacacacccg taactcgcgt attccggaag
    26461 gtctggcagg cggtaccgaa gggaaattta acgaaaaagc gacacaggat ttcgtcgata
    26521 tcgatcttga tgacgtgatg ctgcacagcg aagttaacct gccgattgat ttcctcgtta
    26581 accaaacgct gacgctgggt acggagtgga atcagcaacg gatgaaggac ttgagttcca
    26641 atacccaggc gctgaccggg gcgaacaccg gcggcgctat tgatggtgtg agtgccaccg
    26701 accgtagccc gtattcaaaa gcagaaattt tctcgctgtt tgccgaaaac aacatggagc
    26761 tgactgacag caccatcgta acgccggggc tgcgtttcga tcatcacagt attgtcggca
    26821 ataactggag cccggcgctg aacatatcgc aaggtttagg cgatgacttc acgctcaaaa
    26881 tgggcattgc ccgtgcttat aaagcaccga gcctgtacca gactaacccg aactacattc
    26941 tctacagtaa aggtcagggc tgctatgcca gcgcgggcgg ctgctatcag caaggtaacg
    27001 atgacctgaa agcagaaacc agcatcaaca aagagattgg tctggagttc aaacgcgacg
    27061 gctggctggc gggcgtcacc tggttccgta acgattatcg caataagatt gaagcgggct
    27121 atgtggctgt agggcaaaac gcagtcggca ccgatctcta tcagtgggat aacgtgccga
    27181 aagcagtggt tgaaggtctg gaaggatcgt taaacgtacc agttagcgaa atggtgatgt
    27241 ggaccaataa catcacttat atgctgaaga gtgaaaacaa aaccacgggc gaccgtttgt
    27301 cgatcatccc ggagtacacg ttgaactcaa cgctgagctg gcaggcgcgg gaagatttgt
    27361 cgatgcaaac gaccttcacc tggtacggta agcagcagcc gaaaaaatac aactacaaag
    27421 gtcagccagc ggttggcccg gaaaccaaag aaatcagtcc ttacagcatt gtgggtctga
    27481 gcgcgacctg ggatgtgacg aagaatgtca gtctgaccgg cggcgtggac aacctgttcg
    27541 acaaacgttt gtggcgtgcg ggtaatgccc agaccacggg cgatctggca ggggccaact
    27601 atatcgcggg tgccggggcg tatacctata acgagccggg acgtacgtgg tacatgagcg
    27661 taaacactca cttctgatgc taacgtcaga ttgttgacaa agtgcgcgtc gttcatgccg
    27721 gatgcggcgt gaacgcctta tccggcctac aaaaatcttg ccaattcaat atattgcagg
    27781 accatgtagg cctgataagc gtagcgcatc aggcagtttt gcgtttgtca tcagtctcga
    27841 atatggtcga tatgaaaact acgcatacct ccctcccctt tgccggacat acgctgcatt
    27901 ttgttgagtt cgatccggcg aatttttgtg agcaggattt actctggctg ccgcactacg
    27961 cacaattgca acacgctgga cgtaaacgta aaacagagca tttagccgga cggatcgctg
    28021 ctgtttatgc cttacgggaa tatggctata aatgtgtgcc cgcaatcggc gagctacgcc
    28081 aacctgtctg gcctgcggag gtatacggca gtattagcca ctgtgggact acggcattag
    28141 ccgtggtatc tcgtcaaccg attggcattg atatagaaga aattttttct gtacaaaccg
    28201 caagagaatt gacagacaat attattacac cagcagaaca cgagcgactc gcggactgtg
    28261 gtttagcctt ttctctggcg ctgacactgg cattttccgc caaagagagc gcatttaagg
    28321 catcggagag acaaactgaa gcaggttttc tggattatca gataattagc tggaataaac
    28381 agcaggtcat cattcatcgt gagaatgaga tgtttgctgt gcactggcag ataaaagaaa
    28441 aaatagtcat aactctgtgc caacacgatt aattgacaac atctggtacg attcgcccgc
    28501 agccatcact gactgcgggc gaaagtgtaa agcaggtgcc ttaccactct gacctgacaa
    28561 ccggatatgc agggattgct ccccgcacaa cggctacttc ttcggttcgt aagcgagaac
    28621 agccttaaac tcaatattac gttcctttac ggtgaactca cacagcgagt ctccgaccag
    28681 aagcgtaaat cccagcaccg tcaaacacag cactatgact gccacaaggg catatttcgt
    28741 cagcatgttt atattgcctc catgcgaaga agggactacc atccgagttg ttgaggttcg
    28801 gagtggcagc cccaggattg atagaaatat ctcctggggc ttcatctttc cagacctcag
    28861 agtaaacctg aaaccggaaa gcttcaggca cccgccgtta tcttaccctt cctcagcaaa
    28921 atatctgttt atttatacag taagtcctgt ttcggacttc tgttggcaac actcgttatt
    28981 gctcgtaaaa cgaaatcgcc cgcagccatc actgaccgcg ggcgaaagtg taaagcaggt
    29041 gccctaccat cctgacctga caaccggata tgcggggatt actccccgca caacggctac
    29101 ttcttcggtt cgtaagcgag aacagcctta aactcaatat tacgttcctt caccgtaaac
    29161 tcacacagcg agtctccgac cagaagcgta aatcccagca ccgtcaaaca cagcactatg
    29221 actgccgcaa gggcatattt cgtcagcatg tttatattgc ctccatgcga agaagggact
    29281 accatccaaa ttgttcgggt ttggagtggc agccccaggg ttgatagaaa tatctcctgg
    29341 ggcttcatct ttccagacct cagagtaaac ctgaaaccgg aaagcttcag gcacccgccg
    29401 ttatcttacc cttcctcaac aaaatggctg tttatttata cagtaaatcc tgtttcggac
    29461 ttctcactcc tcatttaatc aactgaagct gagcccgttt tttctgcatg cctcaatcgt
    29521 caaaattctt cgccatcagc cagctctgcc ataacttgcc acattgaaaa agtagccaaa
    29581 ttgcttcagt caccatcatc aaaaaaagaa acattccaga acttccgcat ctcgcccagc
    29641 cgctccgact tctatactga atagaaaacg ccaacataag agaaacctat gccattaccc
    29701 gattttcatg tttctgaacc ttttaccctc ggtattgaac tggaaatgca ggtggttaat
    29761 ccgccgggct atgacttaag ccaggactct tcaatgctga ttgacgcggt taaaaataag
    29821 atcacggccg gagaggtaaa gcacgatatc accgaaagta tgctggagct ggcgacggat
    29881 gtttgccgtg atatcaacca ggctgccggg caattttcag cgatgcagaa agtcgtattg
    29941 caggcagccg cagaccatca tctggaaatt tgcggcggtg gcacacaccc gtttcagaaa
    30001 tggcagcgtc aggaggtatg cgataacgag cgctatcaac gaacgctgga aaactttggc
    30061 tatctcatcc agcaggcgac cgtttttggt cagcatgtcc atgttggctg cgccagtggc
    30121 gatgacgcca tttatttgct gcacggcttg tcacggtttg tgccgcactt tatcgccctt
    30181 tccgccgcgt cgccatatat gcaaggaacg gatacgcgtt ttgcctcctc acgaccgaat
    30241 attttttccg cctttcccga taatggcccg atgccgtggg tcagtaactg gcaacaattt
    30301 gaagccctgt ttcgctgttt gagttacacc acgatgatcg acagcattaa agatctgcac
    30361 tgggatattc gccccagtcc tcattttggc acggtggagg ttcgggtgat ggataccccg
    30421 ttaaccctta gccacgcggt aaatatggcg ggattaattc aggctaccgc ccactggtta
    30481 ctgacagaac gcccgttcaa acatcaggag aaagattacc tgctgtataa attcaaccgt
    30541 ttccaggcct gtcgctatgg gctggaaggc gtcattaccg atccgcacac tggcgatcgt
    30601 cgaccgctaa cggaagacac cttgcgattg ctggaaaaaa tcgccccttc cgcacataaa
    30661 attggtgcat cgagcgcgat tgaggccctg catcgccagg tcgtcagcgg tctgaatgaa
    30721 gcgcagctga tgcgcgattt cgtcgccgat ggtggctcgc tgattgggct ggtgaaaaag
    30781 cattgtgaga tctgggccga tgactaagta gggaattgcc ttacagaaga atatccctga
    30841 caatggtgtt ttaacttaca tttaacaatg ttatgaaaca ccctttagaa accttgacta
    30901 ccgcagcagg cattttgctg atggctttcc tctcttgcct gctgctgccc gcccccgcac
    30961 tggggctgac gctggcacaa aaactggtga ccatgttcca tctgatggat cttagctagc
    31021 tttacacttt attgttttgt ctgtggtttt tagtgctggg cgctattgag tattttgttc
    31081 tgcgctttat ctggcgacgc tggttctcgc tggcggatta aacgtcgatt atcgccgtac
    31141 ttcgcggcat actttgctta ttctccttcg cctaaaggaa cgtttatgga taagcaatca
    31201 ctgcacgaaa cggcgaaacg cctggccctt gagttaccct ttgtcgagct ttgctggcct
    31261 tttggcccgg agttcgatgt ttttaaaatt ggcggcaaaa tttttatgct gtcgtcggag
    31321 ctgcgcggcg tcccctttat caatctgaag tccgatccac aaaaatccct gttaaatcag
    31381 cagatatacc cgagcattaa gccagggtat cacatgaata aaaagcactg gatttcggtg
    31441 tatcccggcg aggaaatctc cgaagcgtta cttcgcgatc tgatcaacga ttcgtggaat
    31501 ctggtggttg atggtctggc taaacgcgat caaaaaagag tgcgtccagg ctaaagcgga
    31561 aatctatagc gcatttttct cgcttaccat ttctcgttga accttgtaat ctgctggcac
    31621 gcaaaattac tttcacatgg agtctttatg gatatcattt ctgtcgcctt aaagcgtcat
    31681 tccactaagg catttgatgc cagtaaaaaa cttaccccgg aacaggccga gcagatcaaa
    31741 actctcctgc aatacagccc atccagcacc aactcccagc cgtggcattt tattgttgcc
    31801 agcacggaag aaggtaaagc gcgtgttgcc aaatccgctg ccggtaatta tgtgttcaac
    31861 gaacgtaaaa tacttgatgc ctcgcacgtc gtggtgttct gtgcaaaaac cgcgatggac
    31921 gatgcctggc tgaagctggt tgttgaccag gaagatgctg atggccgctt tgccacgccg
    31981 gaagcgaaag ccgcgaacga taaaggtcgc aagttcttcg ctgatatgca ccgtaaagat
    32041 ctgcatgatg atgcagagtg gatggcaaaa caggtttatc tcaacgtcgg taacttcctg
    32101 ctcggcgtgg cggctctggg tctggacgcg gtacccatcg aaggttttga cgccgccatc
    32161 ctcgatgcag aatttggtct gaaagagaaa ggctacacca gtctggtggt tgttccggta
    32221 ggtcatcaca gcgttgaaga ttttaacgct acgctgccga aatctcgtct gccgcaaaac
    32281 atcaccttaa ccgaagtgta attctctctt gccgggcatc tgcccggcta tttcccctca
    32341 gattctcctg atttgcataa ccctgtttca gccgtcatca taggctgctg ttgtataaag
    32401 gagacgttat gcaggattta atatcccagg ttgaagattt agcgggtatt gagatcgatc
    32461 acaccacctc gatggtgatg attttcggta ttatttttct gaccgccgtc gtggtgcata
    32521 ttattttgca ttgggtggta ctgcggacct tcgaaaaacg tgccatcgcc agttcacggc
    32581 tttggttgca aatcataacc cagaataaac tcttccaccg tttagctttt accctgcagg
    32641 ggattatcgt caatattcag gcggtattct ggctgcaaaa aggcaccgaa gcggcagata
    32701 ttctgactac ctgcgcgcag ttgtggatca tgatgtatgc gctgctttca gtcttctcgt
    32761 tgctggatgt tattttgaat ctggcgcaga aattcccggc ggcatctcag ttaccgctga
    32821 aagggatatt tcaggggatt aaactgatcg gcgcgatcct ggtcggcatt ttgatgatct
    32881 cgctgctgat tggtcagtcg ccagcgattc tgatcagcgg tcttggtgca atggctgccg
    32941 tgctgatgtt ggtatttaaa gatccgattc ttggtctggt ggccggtatt cagctttccg
    33001 cgaacgatat gctgaaactg ggcgactggc tggagatgcc gaaatacggc gcagatggcg
    33061 cggtgatcga tattgggtta accaccgtca aagtgcgtaa ctgggacaat accattacca
    33121 ctattcccac ctggtctctg gtttctgact cctttaaaaa ctggagcggg atgtcagcat
    33181 ctggcgggcg acgtattaag cgcagtatca gtattgatgt caccagtatt cgttttcttg
    33241 atgaagacga aatgcaacgt ctgaataaag cgcatttgtt aaagccttat ttaaccagcc
    33301 gccatcagga aattaatgag tggaatcgcc agcaaggttc tacggagtcg gtattaaacc
    33361 tgcgccgaat gaccaatatt ggaacctttc gtgcctatct gaacgaatat ctgcgtaacc
    33421 atccgcggat tcgtaaagat atgaccttaa tggtacgcca actggctcca ggtgataacg
    33481 gtttaccgct cgagatctac gcgtttacta acaccgtggt gtggctggaa tatgaaagca
    33541 ttcaggctga tatattcgat cacatatttg cgattgtcga agagtttggt ctgcgacttc
    33601 atcagtcgcc aaccggcaat gatattcgct ctctggcggg tgcatttaag cagtaattaa
    33661 aaaaaccgct ctcatcgaat ggatgagagc ggacatcgtt ttgtcggatg tagcgtgaat
    33721 gccttatttc cgacgcagcg ttttaaatgc cacaaacagg aatacaatcc acaccggcag
    33781 caggatcgct gacaagcgca tatcatccat cgtgcacatc agcagcaaaa tcatgccgag
    33841 gaaggcaatg cagagatagt ttccgaacgg atagagcagc gccttaaact gtgtttcacg
    33901 cccctgacgt cgcatcgctg cacgaaaacg cagatgcgcc agacagatca taatccagtt
    33961 caacagcagc gttgctacca ccagcgccat cagcagacca aacgcttttt gcggcagcag
    34021 atagttgatt aacaccacca gcgaagtgat cgctccggaa agcatcagcg agttaatcgg
    34081 cacaccgcga cggctgacgc gagtcaaaaa cttcggcgca ttaccctgca cagaaaggcc
    34141 aaacagcatg cggctgttag agtaaacccc gctgttatac actgacagcg atgctaccag
    34201 aatgacgaag ttcagcgcag aagctaccac gttgctgtcg agattatgga aaatcatcac
    34261 aaacgggcta ctgttggatt tcacttccac ccacggatag agcgccagta aaaccaccag
    34321 tgaaccgatg taaaacagca ggatgcgata caccacctga tttaccgctt ttggaatgct
    34381 tttatccgga tcgcgcgctt cagcggcagt aatcccaatc agctccagac cgccgaagga
    34441 gaacataatt accgccagcg acaaaatcag cccattccag ccggtggcga agaaaccacc
    34501 gtagcgccag aggttgtcga tactggcttt ctcgccgccg tgaccagaaa acagcagcca
    34561 caggccaaag ccgatcatac cgatgattgc cagcacttta atcaacgcaa accagaactc
    34621 ggtttcgcca tataagcgca cgttcaccag gttaacggcg ttgatgataa taaagaaggc
    34681 ggcagcccaa atccacgttg gaacatccgg gaaccagtac tgcatataga tgcccgcagc
    34741 ggtcagctct gccattccca ccagcacgaa cattacccag tagttccagc cagagaggaa
    34801 gcccgcaaac ggtccccagt atttataggc aaagtgggca aatgaaccgg ataccggctc
    34861 ttcgaccacc atctcgccaa gctggcgcat aatcaggaaa gcgatgatcc cggcgacgcc
    34921 gtagcccagc aatacagccg gacccgccat ctgaatcgcc ggaccaatgc caagaaacag
    34981 accagtacca attgcgccac ccagcgcaat cagttgaata tgacggttat gtaatccgcg
    35041 atgaagcgtc ggctcttgat tcgacgcagt atcttccgat acggttgacg cgtttttcac
    35101 gcctttcccc tgtgtgtctt ttttgttgag gggcaccttt taacatttag tgcccatcgt
    35161 cgcaagacac aatccacacg gttaaacggg gtatcctgct tttatttccg tacccgatgt
    35221 cggtgcagcc acatcagctt atacgccgcc gggataataa acagcgacag caaaggtgcg
    35281 gtgatcatgc cgccaatcat cggtgcggca atccggctca tcacctctga accagccccc
    35341 gttccccaca gaatcggcag cagaccggcg ataatcaccg ccaccgtcat cgctttcggg
    35401 cgcacgcgca gtaccgcgcc gtgatacaac gcctcatcca gcttctgctc actgaatgtt
    35461 tgcggattat tcaacgacgg ctcggcctct atggcgtgac gtaaatacat cagcatcacc
    35521 acgccaaatt cggcggcgac cccggcaagg gcaataaaac cggtgcccgt tgccacagaa
    35581 agatgaaagc ccatccacca cagcaaccag atgccgccca ccagcgcaaa cggtacgctg
    35641 ctgataatca gcaacgcttc gcccacccga cggaatgcca gatacaacag cacgaagatg
    35701 atcatcagtg tcatcggcac catgagtttc agcttatggt tggcgcgctc cagtagctcg
    35761 aactgcccgg agaatgccac gctggtgccc ggttttaact gcactttttc agctatcgct
    35821 ttttgcaaat cgtgaaccac cgacaccatg tcacgatcgc gggcatcgat ataaatccag
    35881 ctcgtcgggc gcgcattctc ggttttcagc atcgacggtc cggtagagac tttaacgtcg
    35941 gccacgtctg ccagggtgat ttgctgcttc atcggcgtca ggatcggtag ctggcgcagt
    36001 gcctgcgggc tatcgcgcca gctttgcgga taacgcagat taatgggata acgggcaatc
    36061 ccttccaccg tttcgccaac catcgccccg cccaccgcag aagtcacaaa caactgcaca
    36121 tccgccaccg tcataccgta acgcgcggct ttttcacggt taatctcaac gttgatatag
    36181 cgcccacctt ccagacgctc ggctagagca gaagccacgc ttggcaccgt tcgcgctact
    36241 tcttcaattt gctcagccat cgcgtcgata tccgccagca cagtgccgga aactttaatg
    36301 ccgatggggc ttttaatgcc ggttgagagc atatcgatac ggttacgaat tggcggcacc
    36361 cacagattcg ccagccccgg cagccgcacg gtgttatcca gttcctcaat gattttgtcc
    36421 atcgtcatgc caggccgcca ctgatcctgc ggcttaagct ggatggtcgt ttcaaccatc
    36481 tcgagcggag cagaatcggt ggcggtttcc gctttcccgg ttttgccaaa tacccgcgcc
    36541 acttcaggta cgctcataat tagcttgtcg gttttttgca gcatactcgc cgcctctgct
    36601 gcggaaatcc ccggcagcgt cgatggcata tacaacaagt cgccttcatt gatctgcggt
    36661 aaaaattccc cgccaacttt attgagcggc cagagaaccg tcagcaccga aagcgctgcc
    36721 accaacagcg tggttttcgg ccagtgcagt actttcagca atagaggatg ataaacacga
    36781 atcaaaaagc gattgagcgg gttactgctt tccggcggaa ttttgccacg gatccagtac
    36841 cccatcagga tagggatcac tacgatcgcc agcagcgccg cgcccgccat cgcataggtt
    36901 ttagtgaacg ccaacgggcc aaacagacgc ccttcctgcc cttccagggt gaagatcggg
    36961 ataaacgaca acgtgataat cagcagactg ataaatagcg ccggacccac ttcaacagat
    37021 gcatcggtga tcacctgcca gcgcgtttta ttatccagcg tggcgtcagg atgctggtgc
    37081 tgccactctt ccagccgttt atgtgcattt tcgatcatga cgatagcggc atcgaccatc
    37141 gccccgacgg caatcgcaat gccgcccaac gacataatat tggcattcag tccctggaag
    37201 tgcatgacaa taaaagcaat acacaacccc agcggcaacg aaataatcgc caccagcgcc
    37261 gagcgcacat gccagagaaa cagcgcacag actaccgcca ccacgataaa ctcttccagc
    37321 aacttgccgc tgaggttgtc gatggcgcgg tcaatgagct ggctgcgatc gtatgttgta
    37381 actatctcca cgccttccgg cagactactt ttcagcgttt ccagtttgtc cttcacggag
    37441 gcgatcactt ctcgggcgtt tttgccggag cgcaggatca ccaccccgcc cgccacttcg
    37501 ccttcgccat tcagttcggc aatgccccgg cgcatctccg ggccaatctg gaccttcgca
    37561 acatcacgca gataaacggg cacgccattt tcactggctt ttaaaacgat gtgattaaag
    37621 tcgtcgagcg tttgcagata gccgctggcg cgcaccatat attccgcttc cgccagttcg
    37681 atcgacgaac cgcccgcttc ctggtttgaa gcatccagcg cgctttttac ttcggctaga
    37741 ctgatgccat attgcgccag gcgctgggga tcgataacca cctgatactc tttcaccacg
    37801 ccgcccaccg acgccacttc cgcaacgtca gggatggttt ttagctcata tttgagaaac
    37861 cagtcctgca atgagcgtaa atcggccaga tcgtgcttac cgctgcgatc caccagtgca
    37921 tattcataga tccagccaac acccgtggca tctggtccca gctcggcgct gactcccgca
    37981 ggcagcttac cctgtacctg gttgaggtac tccagcaccc gcgaacgcgc ccagtacgga
    38041 tcggtaccat cttcgaaaat gacatacaca taagagtctc caaactgcga gaaaccgcgc
    38101 acagtcttcg cgccaggcac cgacaacatg gtggtggtta gcggataagt cacctgattt
    38161 tcaacgattt gcggtgcctg accgggatag ctggttttaa taatcacctg cacatcggag
    38221 agatccggca gcgcatccac tggcgtgtta atgatggtcc aggtgcccca gatgctcaga
    38281 aacaacgcgc ccatcagcac caaaaaacgg ttcgccaccg agcgacgaat aatccattca
    38341 atcattggtt attccctcaa tgcgcattgg tagcactttc agagcgcatc cgctccagtg
    38401 cgccagaaat attggcttcg gaatcaatca ggaacaggcc gctggaaacc accttttcac
    38461 cttccgccag accagagtgt aacgcggtga cgccttgcga tgcctggaaa acggcaacgc
    38521 gtttcggtac aaagcgtccg tcggcatcaa cggtaatcac ccgctgttcg ttgccggtat
    38581 caatcagcgc ctgtgacgga atgagcagca tcggttcgct ggcggtgttg agttgcaacc
    38641 aggcgttcat gcccggtttt agcgcctcgt cggcgttgtc gacttccaga cgcagctgca
    38701 gcgtgcgggt cgcggcatcc acgccaggta gcagcgtcca tttgcggatg gtgagtgttt
    38761 tatccggtcg cgccggaacg gtgagcgtaa actgcgaggc atctttcacc agccaggcga
    38821 tagactccgg gatcgcagca gtgacccaca ccgggtccat accctgaatt ttcgctacca
    38881 cgttatcttt ggcgatattc attcccgcgc gcagatcaaa cgcggtgatc acgccatcaa
    38941 tgggtgcttt gagcgtaaag cgagtctgga ttttttgcgt ggcgatcagg cggcgaatat
    39001 ccgcctccgg cattcccgcc agtcgcagcc gctcaagaat gccttcagtc tgggtcgccg
    39061 taccgccggt ttcgcgcagc agtaaatact cactctgcgc ttccacccag tcaggaatgg
    39121 tcaggtcgag aagcggtgtg cccttttgta ctttatcgcc cacggtaagc ggatacacct
    39181 tgtcgataaa cccggcagcg cgagcctgca caatggcata ctgatactcg ttgtaactga
    39241 cattcgccgg gaaactctgg gcaaaagtca gcggtccgcg cgtgacggta gccgttttca
    39301 cccccagatt ctgcgtctga gtcgggtcaa tgcgcacacc agacgcagaa ctctcttcat
    39361 cggcatattt cggcaccaga tccatatcca taaacggcga tttacctggt ttatcgaacc
    39421 gcgtattggg gtacatcggg tcgtaccaga ataagatttt acgttctgcg gtcgacgttt
    39481 tttctgcggg tggttcagcc tttgcaaacc aggtaaaacc tgccgcagaa ataataccgc
    39541 ccgcgatcat gctgccgata ataagcgcga tttttttcat ctcattaaac ctgggttact
    39601 ggctgacttt aatatcctgt aataaagaaa ggttgccctg ctggacaaaa ttaaacgcca
    39661 ctttgtcgcc ggttttaatt tcactcattt tcgtctgcgg ggtgatggta aagcgcatag
    39721 tcatctccgg ccagttcacg gcagcaatcg gatcgtgatg gatggtgatt tttttgcttt
    39781 ccagatcaat accctttacc acgccagtgg cgctaataac ctgcggttgt acttcgctca
    39841 tggtttcatg atgatgttcg ttagcctggg cattaaagcc aataacggta aacagactga
    39901 acattgcgac ttgcagtgct tttttcattt attctctcct ggagttaaaa attattgctg
    39961 ccaaccgccc cctaaggcgg tatacaaaga aatttcgtta acctgacggg cataattcag
    40021 gtcgagtaaa gtttgtcggg ttgcaaataa agaacgctcg gcatccagca cttccagata
    40081 acttaccgcg ccgtgctgat ataatgcccg cgcccgttgc aaagtaattt gcagcgacgc
    40141 cagataacgc tgctgggcgc tgctttgatc gttcaggctt tgacgtaatg ccagcgcatc
    40201 tgccacttct ttaaaggcgt tctggatttt ctgttcataa ttcactaccg actgctgctg
    40261 gcgaatttcg gcgatatcca gattggcctg gttgcgtccg gcattaaaaa tggggatctc
    40321 aattttagga ataaaattcc acatcccgct gctagcgtta aataatgaag acagatcgct
    40381 gctggcggtt gatattccgc tggtcaggct gatagacggg aaaaatgccg cacgcgcagc
    40441 accaatatta gcattagccg ccattaacgc gtgttcagct tccataatat ccgggcgctg
    40501 caataagatt tgcgacgaca agcccgccgg taatttaacg ctttgcaggc tgccgctgtt
    40561 tactgtctgc gcttgcggca gtttgccgta gcttcccaat aacagttgca atgcattatt
    40621 cgcctgcgcc agttccccct gacgtttagc gatgtcactg cgggtacttt ctatcacccc
    40681 gcgagcctgt tccagcgcca gaacattgct gctaccggtc aacagttgtt tttcgacaaa
    40741 cgcatatgac tgctgataat tacgcagcgt ttcttcggct atttgcaatt gcgcatacgc
    40801 cagttgctga ttgaaatagc tttgcgcgac attagaaacc agcagaatat gcaccgcgcg
    40861 ctgagcttcc tcagtggcta aataattttg tcgctcggct tcgctcatgt tctttaagcg
    40921 accgaaaaaa tcgaggtcaa agctggcgtt aaggccggtc gaaaactccc gtgtcgtggc
    40981 ggtattccct ttaagattac cgctccagct gccgctgccc tcgccattga gctgtgggta
    41041 gcggtcggca tcggtcagac gatattgcgc ccgtgcctcc tgcactttca gcgtcgccat
    41101 gcgcaaatcc cggttattca ccagcgcctc gctaatcagc gtcttcacct gattatcaac
    41161 aaaaaaggtg cgccagccca cgttctggta gttatctgct gcgttaacca agccgttctg
    41221 gctgagtgag aactgctgcg gcacgggcat tgccgtacgc tgataatccg gtgccaatga
    41281 acaaccggtt agcgcaaggg ccacacaaaa tggcagaagt ttacaaggag acataggctc
    41341 ataatttctg gtgattttat accgccaact ttactcgcca ggctctgatt ttccggtgac
    41401 aggaaaatga caaaattgtc attttgccaa taagcgattg ccatctgatc ccgctactct
    41461 agaattgccc gggcaacatg cggaggaaaa atgaaactgt tgattgtcga agatgaaaag
    41521 aaaaccggag aatacttgac caaagggtta accgaagccg gttttgtggt cgatttggcc
    41581 gacaacgggc tgaatggcta ccatctggcg atgaccggtg attatgacct gataatcctc
    41641 gatattatgc tgccggacgt gaacggctgg gatatcgtgc gcatgttgcg ctccgccaat
    41701 aaagggatgc cgattctgtt gcttaccgcg cttggcacca ttgaacatcg cgtcaagggg
    41761 ctggagttgg gggccgatga ctatctggtg aagccgttcg cttttgctga actgctggcg
    41821 cgggtgcgca ctctcctgcg gcgcggggcg gcggtgatta tcgaaagtca gtttcaggtt
    41881 gccgatctga tggtcgatct cgtcagccgc aaagtcaccc gcagcggcac gcgtatcact
    41941 ctgaccagta aagagtttac tctgctggag ttttttctcc gccatcaggg cgaagtgctg
    42001 ccccgctcgc ttatcgcctc gcaggtgtgg gacatgaatt tcgacagcga cactaacgcc
    42061 attgatgtgg cggtgaagcg gctacgcggc aaaatcgaca acgactttga gccgaagctg
    42121 attcagaccg tgcgcggcgt gggctatatg cttgaggtgc cggatggtca gtaagccatt
    42181 tcagcgcccg ttttcgctgg caacccgtct gacctttttt atcagcctgg ccaccatcgc
    42241 ggcgtttttc gcctttgcat ggatcatgat ccactcagtg aaagtgcatt ttgccgagca
    42301 ggatattaat gatttaaaag aaattagcgc cacccttgaa cgggtactta atcatcctga
    42361 cgagacgcaa gcccgacgct taatgacgct ggaagatatc gtcagtggtt attccaacgt
    42421 gttgatttcc ctggcagata gccacggcaa aacggtgtat cactcccccg gtgcgccgga
    42481 tatccgcgag tttacgcgtg acgccatacc cgataaagac gctcagggtg gcgaggtgta
    42541 tctcctttcc ggcccgacga ttatgatgcc aggccacggt cacgggcata tggaacacag
    42601 caactggcgg atgattaact tgccggttgg cccgttggtg gacggcaaac cgatttatac
    42661 gctctacatc gcgctttcga tcgattttca tcttcattac ataaatgatt tgatgaataa
    42721 acttattatg accgcatctg tcatcagcat cctgatcgtc tttatcgtgc tattggcggt
    42781 acataaaggt cacgcgccga tccgcagcgt cagccgtcaa atccagaata tcacctcgaa
    42841 agatctcgac gttcgccttg acccgcagac cgagcccatt gagctggaac agctggtact
    42901 gtcgttcaac catatgatcg agcgtattga ggatgtcttt acccgccagt ccaatttctc
    42961 agcggatatc gctcacgaaa ttcgcacgcc gattaccaat ctcatcacgc aaacggaaat
    43021 cgccctcagc cagtctcgca gccagaagga gctggaagat gtgctctact ctaatctcga
    43081 agagctgacg cgaatggcga aaatggtcag cgatatgctg tttctcgctc aggccgataa
    43141 caaccagcta atccccgaaa agaaaatgct caacctggcg gatgaagtcg gcaaagtgtt
    43201 cgattttttc gaggcgttag cggaagatcg cggcgtggag ttgcgatttg ttggcgacaa
    43261 atgtcaggtt gcgggcgatc cgctgatgct gcgtcgggcg ttaagcaacc tgctttctaa
    43321 tgccctgcgt tatacgccaa ccagagagac aattgtagtg cgctgtcaga cggtcgatca
    43381 ccaggtgcaa gttaccgtcg aaaaccccgg tacgcccatt gcgcccgagc acttaccgcg
    43441 attgtttgac cgtttctacc gcgttgaccc ttcccgccag cgaaaaggcg aaggtagcgg
    43501 cattgggctg gcgatagtga aatcgattgt tgtcgcgcat aaaggcacgg ttgcagtaac
    43561 gtcagatgcg cgggggacaa ggtttgtgat tatgctgccg gagagagagt gattattaag
    43621 ttcaaattca gggcgtggtg catcacctcc tggtattgtt gatcaacaaa ttagagaaaa
    43681 acttattaaa catatgaaat atcgaaggct tttatttttc aaagtttcac gagatgtttg
    43741 tatatgaaac ttagttaaat ctgtgtttct tccaattgta gatcacaatg ccctttctaa
    43801 attttcatcg ttataaacaa gccatctatc atataaagca atgaataatt taatcgcgat
    43861 taatcatcat taattgttat attattcact caatctattt gtcaagtgaa tttgataaat
    43921 tcctaattta aaatatccac tattgagcca gtatttaaca acgttaagat gtgcaatgtg
    43981 cagatcatgt tatcatgggt ggagcggtag catatatttc ttatgcaatc attcatttta
    44041 ataatgttaa ttggattatt tattgctgtg aataaaaata tattatgaat attggtttaa
    44101 ctttgcgttt ttgttactcg ttgtgatatg cgtacctgtc ctgaataacg ccaagaaaca
    44161 agaggttgag cttgtgatta tttttttgac tgaacagaca cccgtttctg taaagtaata
    44221 tttaaacaaa attctcacat aaccatagca ttttctgttc cttccctaat aacgtctgaa
    44281 gtcgtcccgc ttatcacatt atccgcagtt gtacggtacg gatcattatt cattttactg
    44341 gaggttgcta tgtcaaccgg attacgtttc acactggaag tggacggcct gccgccggac
    44401 gcttttgcgg tggtttcctt tcatctgaac cagtcactct cttcgctctt ttccctcgat
    44461 ctctccctgg tcagccagca gtttctctcc cttgaatttg cgcaggtgct ggacaaaatg
    44521 gcctacctga cgatatggca gggcgatgaa gtacagcgcc gggtgaaagg cgtggtgacc
    44581 tggtttgaac tcggggagaa cgacaaaaac cagatgctgt acagcatgaa ggtgcacccg
    44641 ccgctgtggc gtgccggtct gcgccagaac ttccgtatct tccagaacga ggacatcaaa
    44701 agcatcctcg gcacgatgtt gcaggaaaac ggggtgaccg aatggagtcc gctgttcagc
    44761 gagccgcatc cttcccgtga gttttgtgtc cagtacggtg agactgatta cgatttcctg
    44821 tgccggatgg cggcggagga aggcatcttc ttttatgagg agcatgctta caaaagtacc
    44881 gaccagagcc tggtgctgtg cgacacagtc cgccatctgc ccgaatcttt tgaaatccca
    44941 tggaacccga acacccgtac cgaggtgagc accctctgca tcagccagtt ccgctacagc
    45001 gcacaaatcc gcccttcttc cgtggtgacc aaagactaca cctttaaacg ccccggctgg
    45061 gccggacgtt ttgaacagga aggccagcac caggactacc agcgcacgca gtatgaagtg
    45121 tatgactacc ccggacgttt caagggtgct cacgggcaga actttgcccg ctggcagatg
    45181 gacggctggc gaaacaatgc agaaaccgcg cggggaatga gtcgctcgcc ggagatatgg
    45241 ccgggacgac gaattgtgct gacggggcat ccgcaggcga acctgaaccg ggaatggcag
    45301 gtggtggcaa gtgaactgca cggcgaacag ccacaggcgg tgccaggacg gcagggagcg
    45361 gggacggcgc tggagaacca ttttgcggtg atcccggcag acagaacatg gcgaccacag
    45421 ccgttgctga aaccgctggt cgacggcccg cagagcgctg tcgtgacagg accggcaggc
    45481 gaggaaatct tctgcgacga acatggtcgc gtgcgggtga agttcaactg ggaccgttat
    45541 aacccggcag accaagacag ttcgtgctgg atccgtgtgg cacaggcgtg ggcaggcacc
    45601 ggttttggcc acctggcgat accgcgtgtg ggtcaggaag tgattgtaga cttcctcaac
    45661 ggcgatccgg accagccgat cattatgggg cgcacctacc accaggaaaa ccgccccccc
    45721 cggcagcctg ccgggaacaa agacgcagat gaccatccgc tccaaaacgt acatgggcag
    45781 cggatttaat gagctgaagt ttgatgatgc gacagggaga gaacaggtct acatccacgc
    45841 gcagaagaac atggataccg aagtgctcaa cgaccgtacc accaccgtaa aacacgatca
    45901 ccgcgaaacc gtaaaaaatg accagacggt cacgatccag gaaggtaacc gccttcttac
    45961 ggtggaaaaa ggccacaaga tcaccggagt actgaaaggg tctttatctg aggatgtctt
    46021 tcaggacaga ggcacgattg ccggttcggt gcatgttgac gctgtaaaca atggtggcga
    46081 aggcgacggt atacaggctt atacggcgat taaggaaatt ttgctggccg tggaggaaag
    46141 caaaattgcg ctgacgccgg atagcattca gctacaggtc ggggaatcga cggtaatcag
    46201 gctgtcgaag gatggcatca ccatcgtggg cggttctgtt ttcatcaact gattcactca
    46261 gattatcagg gagtatttta tgcagttcac ttttaatgaa ggccacattc aactgccgtc
    46321 tcaatggcag gatcagtcaa tgcaggtcct ggtgtccaca gataacagtg gcatcaacct
    46381 ggtcattacc cgcgaagccg ttccacaggg aaccctgaca ccagagctgt accaggaaac
    46441 gctggcgctg taccagggca aacttgatgg gtacaccgaa catgcctgcc gggaaatcac
    46501 tctggcagag gccccggcct ggctgctgga ttattcctgg aacggtccgg aagatgaggg
    46561 gaatcagggg cggattagcc agatagccgt attccagcgg cgtggagata cgctgctgac
    46621 cttcactttc agcacctcct tatccctgaa gaacagtcaa aagacgatgc tgctggaggt
    46681 gataaagagc ttcaccccgt tgccaccgga gaatgacatc caaaaggacc agccgcgctg
    46741 acataaacga gggataacac atgagtgaag gaccaggcgg gccacaggga gcgaccgcag
    46801 gcggtacgct ggcaatgcga atgctgtcac agcaggcgat ggtcgccagc cagatgaaac
    46861 gggcagccaa cgacaaagcc attgcacaga tgttggcatc aaaaaagtct ggcccccccg
    46921 ccgccaggct gggcgatgaa attcagcaca agagtttttt gggggcgctg gcaggggccg
    46981 tactgggggc gatagtgacc atcgcagaag gttgcctgat tatggccgcc tgtgccaccg
    47041 gcccttatgc gctggttctg gtacctgcgc tgatgtatgc cagctataag gcgagtgatt
    47101 atgtggagga gaaacagaac cagcttgaat catggataaa cagcttttgt gacacggacg
    47161 gcgccatcaa taccggttct gaaaacgtaa aaattaacgg cgagctggcc gcacgtgcag
    47221 cggtcaccct tccccctcct cccccacctg gagcaatacc tgaagtccca cagggggaac
    47281 cctcatgggg tgatattgcc actgacctgc ttgaatcggc agcggaaaaa gcagtaccac
    47341 tggcgaaggc ctgggggaac gctgttatca ccctgacgga cagcaatgcc ggttttatgg
    47401 atcgcgtcag cgccggcgca tcgcttctgt ttcccgccgg tccggtatta atggagtttg
    47461 ccaccatggt gggcgggcgt ggcgaaatca aaaaagatgt ggatttcccg gaagccggtg
    47521 aggacacggc gctctgcgac aaggagaaca aaccaccgag gatagcccag ggcagcagca
    47581 acgtctttat caacaatcag cctgccgcgc gcaagggcga caaactggag tgcagtgcgg
    47641 cgatcgtggg aggttcgccg gacgtcttta ttggcggtga gcaggtcaca tatctggata
    47701 tccagccgga gttcccgccg tggcagagaa tgatcctggg gggaataacg atagccagct
    47761 atcttctgcc gccagcggga ctactgggaa aactgaagaa tctggcgaga ctcggcaaac
    47821 tgggaaactg ctggggaaaa gcgggaagct gctgggcgca aagctcggcg cgttgctggg
    47881 gaaaacaggt aagtcgttaa aaagtattgc caataaagtc atcagatggg taacagatcc
    47941 tgtcgatccg gtaaccggcg catactgcga cgaacgtacc gacttcaccc tgggccagac
    48001 cctccccctc tccttcaccc gtttccacag ttctgtactg ccgctgcatg gcctgaccgg
    48061 cgtgggctgg agcgactcct ggagcgaata cgcctgggtg cgtgaacagg gaaaccgggt
    48121 ggatatcatc agccagggag ccacgctgag atttgccttc gacggtgaca gtgatacggc
    48181 ggttaacccg tatcacgccc agtacattct gcgccgccgc gatgattatc tggagctgtt
    48241 cgacagggat gcactgagca gccgcttctt ttatgacgcc tttccgggaa tgcgtctgcg
    48301 ccacccggtg actgacgata ccagcgatga ccgcctggca cacagcccca gtgaccggat
    48361 gtacatgctg ggcgggatga gcgacaccgc cagcaaccgc atcacgtttg agcgcgacag
    48421 ccagtaccgg atcacgggtg tcagtcacac cgacgggatc cggcttaaac tgacgtacca
    48481 cgccagcggc tacctgaaag ccattcaccg cacggataac gacatacaga cgctggcgac
    48541 ctacgaacag gatgcgcggg ggcggctgac agaagcggat gcgcggctgg actaccacct
    48601 gttttatgag tacgacgctg cggaccggat catccgctgg tccgataacg accagacgtg
    48661 gagccgtttc acctacgatg aacagggccg gtgcgtgaat gtcaccgggg cggagggcta
    48721 ttacaacgcc acgctggact atggtgacgg ctgcaccacc gtgacggacg gcaagggcat
    48781 tcaccgttat tactatgatc ctgacggcaa tattctgcgg gaagaagcgc cggacggcag
    48841 caccaccatg tatgaatggg atgaattcca tcacctgctg gcccgccact cccctgccgg
    48901 gcgggtggag aaatttgaat acaacgccgc acacggtcag ttaagccgtt acacggcggc
    48961 agacggcgcg gagtggcagt accgctatga tgagcgcggc ctgctcagca acatcaccga
    49021 ccctgccggg cagacgtgga cacagcagtg tgatgaacgc ggcctgccgg taagtctggt
    49081 gtcgccacag ggcgaagaga cccggctggc atacaccgct caggggctgc tgtcgggaat
    49141 attccgccag gatgaacggc gtctgggcat agagtacgac caccacaacc ggccggaaac
    49201 actcaccgac gtgatgggcc gtgaacacca caccgaatac agcggtcacg acctgccagt
    49261 gaagatgcgc ggccccggcg gtcagtcagt gcggttacag tggcagcagc accataaact
    49321 gagcggcatt gaacgtgctg gaaccggcgc ggaaggattt cgctatgacc gccacggcaa
    49381 cctgctggcc tggacggacg gcaacggtgt tgtctggacg atggaatacg gtccgttcga
    49441 ttttccggtg gcgcgaacgg acggtgaagg ccaccgctgg cagtaccgct acgataaaga
    49501 cacgctgcaa ctgacagaag tcatcaaccc gcagggtgag tcatatcttt atgttctgga
    49561 taactgcggc cgggtgacgg aagagcgtga ctggggcggc gtggtctggc gttaccgcta
    49621 tgacgccgat ggcctctgta ccgccagggt taacggcctg gaggaaacca tcctctacag
    49681 ccgggacgcc gcaggccgcc tggcagaaat catctcgccg gaaggcaaaa cgcagtatgc
    49741 ctatgacaaa tccggcaggc tgacgggtat cttcagcccg gacggcacat cacagcgcac
    49801 cggctatgac gaacgcgggc gggtgaatgt caccactcag ggccgacggg ccattgaata
    49861 caactacccc gatgaacaca ccgtcatccg ctgtatcctg ccaccggaag atgaacgcga
    49921 cagacacccc ggtgaatccc tgctgaaaac cacgtaccgc tacaacgccg ccggagaact
    49981 gacagaggtc attctgccgg gggatgagac gctgacgttc agccgtgatg aggcgggacg
    50041 tgaagtgctc cggcacagta accggggttt tgcctgtgaa cagggctgga atgcagccgg
    50101 tcagcctgtc agtcagcgcg ccggattttt tccggcagaa gccacatggg acgggctggt
    50161 tccctcactg gtacgggagt accgttacga cagcgcgggt aacgtgtcag gcgtcaccag
    50221 ccgggaagat tacggacggg aaacacggcg ggagtatcgg ctggaccgga acggccaggt
    50281 cacggcggtg acagcctcag gcaccgggct gggctatggc gaaggcgatg agtcctatgg
    50341 ctatgacagc tgcggctacc tgaaggcgca gtctgcgggc aggcaccgga taagtgaaga
    50401 gactgaccag tatgccggag gccaccggct gaaacaggcc ggaaacacgc agtatgacta
    50461 tgacgccgca ggccggatgg tcagccgcac cagacaccgt gacggctacc gaccggaaac
    50521 agagcggttc cggtgggaca gccgggacca gctgaccggg tattgcagcg cacagggtga
    50581 gctgtgggaa taccgccacg acgccagcgg cagacgaaca gagaaacgct gcgaccggaa
    50641 gaaaatccgc ttcacgtatc tgtgggacgg cgacagtatt gcggaaatcc gggaataccg
    50701 cgatgataaa ctgtacagcg taaggcacct ggtgtttaac ggctttgagc tgataagcca
    50761 gcagttcagc cgggtacggc aggcgcatcc gtcggtggcc ccgcagtggg tgacgcggac
    50821 gaatcatgcg gtgagcgacc tgacgggccg cccgctgatg ctctttaaca gtgaaggaaa
    50881 gacggtctgg cgaccggggc agaccagcct gtgggggctg gcactcagcc tgcccgcaga
    50941 caccggctac ccggacccgc gcggggaact ggacccggaa gccgaccccg gcctgctgta
    51001 tgcaggacag tggcaggatg cggaatccgg gctgtgctat aatcggttcc ggtattacga
    51061 gccggaaacc ggaatgtacc tggtgagtga tccgctgggg ttgcagggcg gggagcagac
    51121 ttaccggtat gtgccgaatc cttgtgggtg ggttgatccg ctgggattgg ctgcaagttc
    51181 taaaatcagc agtttgatgg actatattgg cgatggtcgt cgtgttagtg ggcatacggg
    51241 tttcctggat ggggttcgtt tatcacgtag tcaaataa
//
LOCUS       DAESHI010000019        46701 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.019, whole genome shotgun sequence.
ACCESSION   DAESHI010000019 DAESHI010000000
VERSION     DAESHI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46701
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.019"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..135)
                     /gene="tabA"
                     /locus_tag="KW011_001128"
     CDS             complement(<1..135)
                     /gene="tabA"
                     /locus_tag="KW011_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HBH7659525.1"
                     /translation="MIIGNIHNLQPWLPQELRLAIEHIKAHVTAETPKGKHDIEGNRL
                     F"
     gene            complement(280..873)
                     /locus_tag="KW011_001129"
     CDS             complement(280..873)
                     /locus_tag="KW011_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH7659526.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            944..1657
                     /gene="bdcA"
                     /locus_tag="KW011_001130"
     CDS             944..1657
                     /gene="bdcA"
                     /locus_tag="KW011_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBH7659527.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1788..2183
                     /locus_tag="KW011_001131"
     CDS             1788..2183
                     /locus_tag="KW011_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBH7659528.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2464..2598
                     /gene="pyrL"
                     /locus_tag="KW011_001132"
     CDS             2464..2598
                     /gene="pyrL"
                     /locus_tag="KW011_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HBH7659529.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2602..3537
                     /gene="pyrB"
                     /locus_tag="KW011_001133"
     CDS             2602..3537
                     /gene="pyrB"
                     /locus_tag="KW011_001133"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBH7659530.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3550..4011
                     /gene="pyrI"
                     /locus_tag="KW011_001134"
     CDS             3550..4011
                     /gene="pyrI"
                     /locus_tag="KW011_001134"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HBH7659531.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4084..4470
                     /gene="ridA"
                     /locus_tag="KW011_001135"
     CDS             4084..4470
                     /gene="ridA"
                     /locus_tag="KW011_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HBH7659532.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="KW011_001136"
     CDS             complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="KW011_001136"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBH7659533.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="KW011_001137"
     CDS             complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="KW011_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HBH7659534.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7751..8698
                     /gene="treR"
                     /locus_tag="KW011_001138"
     CDS             7751..8698
                     /gene="treR"
                     /locus_tag="KW011_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HBH7659535.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8817..10238
                     /gene="treB"
                     /locus_tag="KW011_001139"
     CDS             8817..10238
                     /gene="treB"
                     /locus_tag="KW011_001139"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HBH7659536.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10288..11943
                     /gene="treC"
                     /locus_tag="KW011_001140"
     CDS             10288..11943
                     /gene="treC"
                     /locus_tag="KW011_001140"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBH7659537.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            12337..14475
                     /gene="nrdD"
                     /locus_tag="KW011_001141"
     CDS             12337..14475
                     /gene="nrdD"
                     /locus_tag="KW011_001141"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBH7659538.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14633..15097
                     /gene="nrdG"
                     /locus_tag="KW011_001142"
     CDS             14633..15097
                     /gene="nrdG"
                     /locus_tag="KW011_001142"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HBH7659539.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="KW011_001143"
     CDS             complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="KW011_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HBH7659540.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="KW011_001144"
     CDS             complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="KW011_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HBH7659541.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17158..17709
                     /locus_tag="KW011_001145"
     CDS             17158..17709
                     /locus_tag="KW011_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HBH7659542.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="KW011_001146"
     CDS             complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="KW011_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HBH7659543.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19341..20339
                     /gene="fbp"
                     /locus_tag="KW011_001147"
     CDS             19341..20339
                     /gene="fbp"
                     /locus_tag="KW011_001147"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HBH7659544.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="KW011_001148"
     CDS             complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="KW011_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HBH7659545.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(21354..22376)
                     /locus_tag="KW011_001149"
     CDS             complement(21354..22376)
                     /locus_tag="KW011_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH7659546.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22390..23892)
                     /locus_tag="KW011_001150"
     CDS             complement(22390..23892)
                     /locus_tag="KW011_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBH7659547.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="KW011_001151"
     CDS             complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="KW011_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HBH7659548.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25298..25828
                     /gene="ppa"
                     /locus_tag="KW011_001152"
     CDS             25298..25828
                     /gene="ppa"
                     /locus_tag="KW011_001152"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HBH7659549.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26207..26548)
                     /locus_tag="KW011_001153"
     CDS             complement(26207..26548)
                     /locus_tag="KW011_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HBH7659550.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="KW011_001154"
     CDS             complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="KW011_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HBH7659551.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="KW011_001155"
     CDS             complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="KW011_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HBH7659552.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32266..32904
                     /gene="msrA"
                     /locus_tag="KW011_001156"
     CDS             32266..32904
                     /gene="msrA"
                     /locus_tag="KW011_001156"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBH7659553.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33227..34570
                     /locus_tag="KW011_001157"
     CDS             33227..34570
                     /locus_tag="KW011_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBH7659554.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34667..34873)
                     /locus_tag="KW011_001158"
     CDS             complement(34667..34873)
                     /locus_tag="KW011_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HBH7659555.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35198..35752
                     /locus_tag="KW011_001159"
     CDS             35198..35752
                     /locus_tag="KW011_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBH7659556.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35842..36753)
                     /locus_tag="KW011_001160"
     CDS             complement(35842..36753)
                     /locus_tag="KW011_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659557.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIHEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPV"
     gene            complement(36965..37705)
                     /gene="cysQ"
                     /locus_tag="KW011_001161"
     CDS             complement(36965..37705)
                     /gene="cysQ"
                     /locus_tag="KW011_001161"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HBH7659558.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            37895..39838
                     /gene="cpdB"
                     /locus_tag="KW011_001162"
     CDS             37895..39838
                     /gene="cpdB"
                     /locus_tag="KW011_001162"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HBH7659559.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSNKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(39956..40336)
                     /locus_tag="KW011_001163"
     CDS             complement(39956..40336)
                     /locus_tag="KW011_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH7659560.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            40425..41285
                     /gene="qorB"
                     /locus_tag="KW011_001164"
     CDS             40425..41285
                     /gene="qorB"
                     /locus_tag="KW011_001164"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBH7659561.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            41393..42358
                     /locus_tag="KW011_001165"
     CDS             41393..42358
                     /locus_tag="KW011_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBH7659562.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            42466..43128
                     /gene="ytfE"
                     /locus_tag="KW011_001166"
     CDS             42466..43128
                     /gene="ytfE"
                     /locus_tag="KW011_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HBH7659563.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(43173..44585)
                     /gene="cycA"
                     /locus_tag="KW011_001167"
                     /gene_synonym="dagA"
     CDS             complement(43173..44585)
                     /gene="cycA"
                     /locus_tag="KW011_001167"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HBH7659564.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(44894..45514)
                     /gene="fklB"
                     /locus_tag="KW011_001168"
     CDS             complement(44894..45514)
                     /gene="fklB"
                     /locus_tag="KW011_001168"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBH7659565.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            45733..46371
                     /gene="ytfB"
                     /locus_tag="KW011_001169"
     CDS             45733..46371
                     /gene="ytfB"
                     /locus_tag="KW011_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HBH7659566.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(46355..>46701)
                     /locus_tag="KW011_001170"
     CDS             complement(46355..>46701)
                     /locus_tag="KW011_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH7659567.1"
                     /translation="PWFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGKK
                     HNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILS
                     WLLVDPSSTAHE"
BASE COUNT        11458 a        12420 c        11635 g        11188 t
ORIGIN      
        1 aaacagacga ttgccttcga tatcgtgctt gccctttggc gtttctgccg taacgtgtgc
       61 tttgatatgc tcaatcgcca ggcgtaactc ctggggtagc cacggctgaa gattatgaat
      121 atttccgatg atcatctttt ccactcctta attaaaacaa tgtttcattt ccatcgttat
      181 acgaaaagat gatcaattcc gctagcctta atgcgtgaat tattgtctta tcacaactta
      241 tcggcaggcg cgttcccgcc tttcgggtca aggcccgcat cactccttga gaatagtttt
      301 tatcgcttcc cccgccagtg cagctgttgc acagagttgt tcgatggagt gcccctcccg
      361 agccttcgca gaaagccctg acatcacggt actcataaaa tcagtcacac attgtgcgct
      421 ttgtggatgc cgcctggcga tatagtcata aatggccgtt tctgcggcgt gataatattg
      481 aacggcaata tcacgcgctt gtggatcatg actatgaatg ccttcaagaa ccatacagcc
      541 agcgcagccg ccgttttggc tatatctgcg cgccgcttct tttaatacct cagccaggca
      601 ctcccctacc ggacgatcat cacgaagaat atcggcaagc ggaatagctt ccgtaccgac
      661 atattcattg agtacacggc taaataaccc agccttactg ccaaaagccg cgtagaggct
      721 cggggggtta ataccaagat aatcagtaac ttcagcaaca ctgacagcat cgaaaccttt
      781 ttggtgaaaa agcacttttg ccgcagagac tgcctgctca ggagcgaaac gtctgggacg
      841 acctggaacg cgagattgtt ttttagtgac cattacaaaa cttgttgaca gaaagttaaa
      901 acagttttgt aatgcatgtt acataataaa tcaaggagtc cttatgggcg cttttacagg
      961 taagacagtt ctcatcctcg gtggcagtcg tggtatcggt gccgctatcg tacgtcgttt
     1021 cgtcaccgat ggggccaatg tacgattcac ctatgcgggg tcgaaagatg ccgctgaaca
     1081 cctggcacaa aagactggag cgacagcagt attcacagat agtgctgaca gagacgctgt
     1141 cattgatgtc gttcgtaaga gcggcgcatt ggatatcctg gtggtaaatg caggtattgg
     1201 cgtctttggc gatgccctgg aattgaatgc cgacgatatt gatcgccttt tcaaaatcaa
     1261 tattcatgct ccttatcatg cctctgttga agccgcccgg cagatgcccg aaggcgggcg
     1321 catcttaatc atcggctccg tgaatggcga tcgtatgcct gttgcaggca tggctgctta
     1381 tgccgccagt aaatctgccc tgcaaggcat ggcgcgcgag ctggcccgtg atttcggacc
     1441 gcgtgggatc accatcaacg tcgtccagcc agggccaatt gataccgacg ctaatcccgc
     1501 caacgggcca atgcgcgata tgttgcatag ttttatggct atcaaaagac atgggcaacc
     1561 ggaagaggtc gctggtatgg tcgcatggtt agcagggcca gaagccagtt ttgttaccgg
     1621 cgcgatgcat accattgatg gcgcgtttgg cgcataaccg actacgctca attaagccca
     1681 gccatttccc atgatgtctg ggttttgttt actcacgtcg tccgctaaaa gcggctcctg
     1741 gtaaatataa atcttctgtt catgtcgtaa atcttgagga taaccaaatg gtagaaagaa
     1801 ccgctgtttt ccctgcaggc cgacattcac tgtatgctga acatcgttat tctgcggcta
     1861 ttcgttccgg cgatttgctg tttgtttccg ggcaagtagg aagtcgagag gacggaacac
     1921 cagaacccga ttttcagcaa caagtcagac tggcatttga taatttgcat gcgaccctgg
     1981 ctgctgcggg atgcactttt gacgatatca ttgatgttac gagcttccat accgatccag
     2041 aaaaccaatt tgaagacatc atgacggtga aaaatgaaat atttagcgcc ccaccttatc
     2101 caaactggac ggcggtaggt gttacatggc tggcaggctt tgattttgaa attaaagtga
     2161 tagcgcgcat ccctgagcag taagcaatag tgttagccgt tcgttttcac actccgccct
     2221 ataagtcgga tgaatggaat aaaatgcata tctgattgcg tgaaagtgaa aaaggaaaaa
     2281 gcaggaaatg tctgcaatta ttgataccga acgaccgttc ccctgcagaa tcacaacaaa
     2341 taaaaatgca tgtattttga cttataattc aaataaaccg tttgcgctga caaaatattg
     2401 catcaaatgc ttgcaccgct tctgacgatg agtataatgc cggacaattt gccgggagga
     2461 tgtatggttc agtgtgttcg acattttgtc ttaccgcgtc tgaaaaaaga cgctggcctg
     2521 ccgtttttct tcccgttgat cacccattcc cagcccctca atcgaggggc ttttttttgc
     2581 ccaggcgtca ggagataaaa gatggctaat ccgctatatc agaaacatat catttccata
     2641 aacgacctta gtcgcgatga ccttaatctg gtgctggcga cagcggcgaa actgaaagca
     2701 aacccgcaac cagagctgtt gaagcacaaa gtcattgcca gctgtttctt cgaagcctct
     2761 acccgtaccc gcctctcttt cgaaacttcc atgcaccgcc tgggtgccag cgtggtgggc
     2821 ttctccgaca gcgccaatac atcactgggt aaaaagggcg aaacgctggc cgataccatt
     2881 tcggttatca gcacttacgt cgatgcgata gtgatgcgtc atccgcagga aggtgcggcg
     2941 cgcctggcca ccgagttttc cggcaatgta ccggtactga atgccggtga tggctccaac
     3001 caacatccga cgcaaacctt gctggactta ttcactattc aggaaaccca ggggcgtctg
     3061 gacaatctcc acgtcgcaat ggttggtgac ctgaaatatg gccgcaccgt tcactccctg
     3121 actcaggcgt tagcgaagtt cgacggcaac cgtttttact tcatcgcgcc ggacgcgctg
     3181 gcaatgccgc aatacattct ggatatgctt gatgaaaaag ggatcgcctg gagtctgcac
     3241 agctctattg aagaagtgat ggcggaagta gacatcctgt acatgacccg cgtgcaaaaa
     3301 gagcgtctgg acccgtccga gtacgccaac gtgaaagcgc agtttgttct tcgcgccagc
     3361 gatctccaca acgccaaagc caatatgaaa gtgctgcatc cgctgccgcg tgttgatgag
     3421 attgcgacgg atgttgataa aacgccacac gcctggtact tccagcaggc aggcaacggg
     3481 attttcgctc gccaggcgtt actggcactg gttctgaatc gcgatctggt actgtaaggg
     3541 gaaatagaga tgacacacga taataaattg caggttgaag ctattaaacg cggcacggta
     3601 attgaccata tccccgccca gatcggtttt aagctgttga gtctgttcaa gctgaccgaa
     3661 acggatcagc gcatcaccat tggtctgaac ctgccttctg gcgagatggg ccgcaaagat
     3721 ctgatcaaaa tcgaaaatac ctttttgagt gaagatcagg tagatcaact ggcattgtat
     3781 gcgccgcaag ccacggttaa ccgtatcgac aactatgaag tggtgggtaa atcacgcccc
     3841 agtctgccgg agcgcatcga caatgtgctg gtctgcccga acagcaactg tatcagccat
     3901 gccgaaccgg tttcttccag ctttgccgtg cgaaaacgcg ccaatgacat cgcgctcaaa
     3961 tgcaaatact gtgaaaaaga gttttcccat aatgtggtgc tggccaatta attgcggttg
     4021 gtaataaaag tctggctccc tataatgagc cagacttttt accgctgtaa taaaggagaa
     4081 atcatgagca aaactatcgc gacggaaaat gcaccggcag ctatcggtcc ttacgtacag
     4141 ggcgttgatc tgggcaatat gatcatcacc tccggtcaga tcccggtaaa tccgaaaacg
     4201 ggcgaagtac cggcagacgt cgctgcacag gcacgtcagt cgctggataa cgtaaaagcg
     4261 atcgtcgaag ccgctggcct gaaagtgggc gacatcgtta aaaccaccgt gtttgtgaaa
     4321 gatctgaacg acttcgcaac cgttaatgct acctacgaag ccttcttcac cgaacacaat
     4381 gccaccttcc cggcacgttc ttgcgttgaa gttgcccgtc tgccgaaaga cgtgaaaatt
     4441 gagatcgaag cgatcgctgt tcgtcgctaa tcttgatgga aatccgggct atcatgcccg
     4501 gattaagtct gatgacaaac acaaaatcgc ctgatgcgct acgcttatca ggcctacgtg
     4561 attcctgcaa tttattgaat ttgttggccg gataaggcat ttacgccgca tccggcatga
     4621 acaaaactca ctttgtctgc aatctgaatc caggccacca tgcccagttg attctttatt
     4681 gccagccgta acgacggcta taaaatcctt tcaccagttg ggttaacgtc atataccctg
     4741 ccagaatcgc aaccagccac gggaaatagc ttaacggcag cgcctgcagt tgcagataac
     4801 tggccagcgg tgaaaacggc aatgcgatcc cgacaatcat cacgatcacg gtcatgatca
     4861 ttaacggcca cgatgcacag ctctgaataa acggcacacg gcgggtgcgg atcatatgca
     4921 caatcagcgt ttgcgacagc aagcccacca caaaccatcc cgactggaac agcgtttgcg
     4981 tttccggcgt gttggcatgg aatacccacc acatcaggca aaacgtcaga atatcgaaga
     5041 tcgagctgat cggtccgaag aagatcataa agcgccccag atccgccgga ttccaacgct
     5101 gcggcttttg aatttgctcg tcgtcgacgt tatcaaacgg gatcgccacc tgtgacacat
     5161 catacagcag gttctgaata agcaagtgta acggcaacat cggcaggaag ggcaagaaag
     5221 cactcgccac cagcacgctg aacacattac cgaagttgga gctcgccgtc attttgatgt
     5281 atttcagcat gttggcgaaa gtgcgacgtc cttcaataac cccctcttcc agcaccatca
     5341 ggcttttttc cagcaggatg atatcagccg cttcacgggc aatatctacc gcgccgtcca
     5401 cagaaatgcc gatatccgca gcgcgtaagg cgggcgcatc attaatacca tcgcccataa
     5461 agccaaccac atgcccttcg cgcttcagta aggtcacaat gcgttcttta tgcatcggcg
     5521 tcagacgagc aaacagcgtg gtgcgctgtg cgagatttgc cagttcgtcg tcagatagcg
     5581 tttcaatatc actaccaatg accacctctc ccgcgtccag ccccacttca tggcacactt
     5641 tcgctgcaac taactcactg tcgccagtaa ggatttttac ggtaattcca ctcgctttta
     5701 atgccttcag tgccggagca gttgtctctt ttggcggatc aaggaaagca atatatcctt
     5761 cgaggatcag gtcggattca tccgcccgct ggtaatctcc ttcacgcgct ggcaggtatt
     5821 tcgtcgccac cgcaaccacg cgcagcccct gacgattcag cgtatcagta acccgcttaa
     5881 tcttacgcag catgatgtca tcgagcggca caatctcgcc attgtgacgc acctgcgaac
     5941 acacattgag gatttcctgc aatgcacctt tgcaaaccag ctgatggtgc tcggtatttt
     6001 ctgccactac caccgacatc cggcgacgct cgaaatcaaa cggaatctca tcaatttttt
     6061 gccaacggct ggccagagag cgcgctgact cttcatccgt accttcgagc accgctgtat
     6121 cgagtaggtt tttaagtccg gtctgataat gactgttcaa ccacgcgcta tgcagcacgc
     6181 gttcgctggt tttaccggag atatcggtat gattctccag cacaattttg tcctgtgtca
     6241 gggtgccagt tttatcagtg cacagaatat ccattgcgcc aaagttctga atagcatcca
     6301 gatgtttgac gatcactttc tgtttcgaca gctttactgc gccgcgcgcc agcgtcgagg
     6361 taacaatcat cggcaacatt tccggcgtta agcctaccgc gaccgaaagc gcaaacagtg
     6421 ccgcttccca ccagtcgcct ttggtgtaac cgttgataag caacaccacc ggagccatca
     6481 ccagcataaa gcgaatcagc agcatactga cgcggctgat cccctgctga aaggcattcg
     6541 gctcgctttc ctgctcacta acacgccccg ccagttgacc aaaccaggtg ttggctcctg
     6601 tagcaatcac cattgcttgt gccgtgccgc tcaccacggt ggtgcccata aaacacaggg
     6661 tgtcacactc cagcggattg ctgtgctccg gctggcgagt ggtagcggct ttttccacgg
     6721 gcagagactc accggttaac gacgcctgag cgacgaacag atcccgcgcc tgcaagatac
     6781 gtaaatctgc cgggatcata tcccccgccg ccagttttat aatatcgccg ggcaccagct
     6841 ggtcgatcgg gatctccagc cagccatttt cgcctttgtc gttaattacg cgcagcaccg
     6901 tcgcggtatt gctgaccatc gctttcaggg catctgccgc tttagtggaa cgtgcttcct
     6961 gaataaagtt cagcaacgta gaaatagcga ccattagcgc gataacgccc gcggcaaata
     7021 aatcttcagt ggcgtaagaa atagcgccga gaatggtgag taaaatatta aatgggttgc
     7081 gatagcagac ccataaatgt acccaccacg gcgacggttg ttgtgcgggt aatttatttt
     7141 caccatgttg ttcgcgggca gattccactt ccgcctgatt taacccttcc ggatgggtgt
     7201 cgaacgtttt ccacaattct tcttcgggca tcaccgccat tttcaggcaa tgcgcactta
     7261 aggacggcgg gaccgccgta ttcactgtct gctgcgcgcc aggcaatgga tcgcgatgaa
     7321 ccagacggga aggtaaatgg cgaatgagcc gggtaaaaat ttctttaaac ataaggagtc
     7381 cctccgcact gtctgaaaca gtacagaaat gtcttcaggc gtggcgaagc cttacctgaa
     7441 ggtaataaag ctgagcaggg acgttaaaac gtcacggcct tacgcatccg gtagcgccgt
     7501 gaaaagacgg gcttaccgga aaagttttaa tctccgtcga gggagaggcg tgggatcagg
     7561 ttccatataa cctccggtaa gtgaaaaaag cgccgcaaat aataaggaga atccaataat
     7621 attcgtagca tttatggcga tataatactt gctggcaact aaaccaacga taaaccagac
     7681 tttacccatt gctgaatgca cgggtaaagt taggctcaaa taattaaaca acacgttaca
     7741 ggacaacagg atgcaaaatc ggctgactat caaagacatc gcacgcttaa gcggcgtggg
     7801 gaaatctaca gtttcccggg tgctgaataa cgaaagcggc gtgagccagc gcacccgcga
     7861 gcgtgttgaa gcagtgatga atcagcatgg attttcccct tcccgctctg cgcgcgctat
     7921 gcgtgggcaa agcgataaag tggtcgccat cattgttacc cgtctggatt cgttgtcaga
     7981 aaatctcgcc gttcaaacca tgctgccagc gttctatgaa caaggttacg acccaatcat
     8041 gatggaaagc cagttttccc cgcaattagt tgccgaacat ttgggggtgc tgaaacggcg
     8101 taatatcgac ggcgtagtgc tgttcggttt tactggcata acagaagaaa tgttagccca
     8161 ctggcagtca tcgctggttc tgctggcgcg tgacgcaaaa ggctttgctt cggtctgtta
     8221 tgacgacgaa ggggcaatca agatcctgat gcaacggctg tatgaccagg ggcatcgtaa
     8281 tatcagttat ctcggcgtgc cgcatagtga cgtgacaacc ggtaagcgac gtcacgaagc
     8341 ctacctggcg ttctgcaaag cgcataaact gcatcccgtt gccgctctgc cagggcttgc
     8401 tatgaagcaa ggctatgaga acgtggcaaa agtgattacg cctgaaacta ctgcgttact
     8461 gtgcgcaacc gacacgctgg cacttggcgc aagtaaatac ctgcaagagc aacgcatcga
     8521 caccttgcaa ctggcgagcg tcggtaatac accgttaatg aaattcctcc atccggagat
     8581 cgtcaccgta gatcccggtt acgccgaagc tggacgccag gcggcctgcc aattgatcgc
     8641 acaggtaacc gggcgcagcg aaccgcaaca aatcatcatc cccgccaccc tgtcctgatc
     8701 gtttcctgaa cgataaattg tgatcttcgc tgcatttcgg gaacgttccc gtttttaaat
     8761 ttttccgcgc aatatattct gcagccaacc aaaaatgtca tctgccatgg ggctttatga
     8821 tgagcaaaat aaaccaaacg gatatcgatc ggttgattga actggtcggc gggcgcggca
     8881 atattgcgac ggtgagccac tgtattactc gcctgcgctt tgtcctcaac caaccggcca
     8941 atgccagacc gaaagaaatt gagcaactcc ccatggtgaa aggctgtttc accaatgccg
     9001 ggcaatttca ggtggtgatt ggcaccaacg tgggtgatta ctatcaagca ctaatagcgt
     9061 caaccggaca ggcgcaggtt gataaagagc aggtaaaaaa agccgcccgg cagaatatga
     9121 aatggcatga gcagttgatc tctcatttcg cggagatctt cttcccgttg ctgcccgcgt
     9181 tgattagcgg cggtttgatc ctcggttttc gcaatgtgat cggcgatttg cccatgagca
     9241 acggtcagac gctggcgcaa atgtaccctt ccctgcaaac gatctacgat tttctgtggt
     9301 tgatcggtga agcgattttc ttctacctgc cggtcgggat ttgctggtca gcggtgaaaa
     9361 aaatgggcgg cacgccgatc cttggtatcg tgcttggcgt gacactggtt tccccccagc
     9421 tgatgaacgc ttatctgctc gggcagcagc tgccggaagt gtgggacttt ggcatgttca
     9481 gcatcgccaa agtgggctat caggcgcagg tgatcccggc actgttagcc gggctggcgc
     9541 tgggcgttat tgaaactcgc cttaaacgca tcgtaccgga ttacctctat ctggtggtgg
     9601 tgcccgtctg ttcgctgatc ctcgcggtgt tcctcgccca tgcgctgatt ggtccgtttg
     9661 gtcgcatgat tggcgatggc gttgcctttg cggtacgtca cctgatgacc ggcagctttg
     9721 ctccgattgg tgcggcattg tttggcttcc tgtacgcgcc gctggtgatc accggcgtac
     9781 accagaccac ccttgctatt gatttgcaga tgattcaaag catgggtggc acgccagtgt
     9841 ggccgctgat tgcgctgtcg aatatcgctc agggctccgc cgtgataggc attatcattt
     9901 ccagccgcaa gcacaatgaa cgcgagatct ccgtgcctgc cgctatctcc gcctggcttg
     9961 gggtcactga gcctgcaatg tacggcatca acctgaaata tcgcttcccg atgctgtgcg
    10021 cgatgattgg ttctggtctg gcaggattgc tatgcggcct gaacggcgtt atggcgaatg
    10081 gtatcggcgt agggggcctg ccgggaattc tctcgattca accgagctac tggcaggtat
    10141 ttgcgctggc aatggctatc gccatcatca tcccgattgt actcacctcg tttatctatc
    10201 agcggaaata ccgcttgggc acgctggaca ttgtttaatt ttcttcgggg cgcaattgcg
    10261 ccccctcgca tttgcaggaa taacgtaatg actaatcttc cccactggtg gcaaaacggc
    10321 gttatctacc agatttatcc aaagagtttt caggacacca cgggtagcgg caccggcgat
    10381 ttacgtggcg ttatccaacg cctggactat ctgcataaac tgggcgttga tgccatctgg
    10441 ctaaccccct tttatgtctc tccccaggtc gataacggtt acgatgtagc gaactatacg
    10501 gcgattgatc ccacctacgg cacgctggac gattttgacg aactggtgac gcaggcaaaa
    10561 tcgcgcggga ttcgtatcat tctcgatatg gtgtttaacc atacctctac ccaacatgcc
    10621 tggtttcgcg aggcgctgaa caaagaaagc ccttaccgcc agttttatat ctggcgcgat
    10681 ggcgaaccag aaacgccacc gaacaactgg cgttcaaaat ttggcggtag tgcgtggcgc
    10741 tggcatgcgg aaagcgaaca gtactatttg catctctttg caccagaaca ggcggatctc
    10801 aactgggaga atccagcggt acgcgcagag ctgaaaaaag tctgtgaatt ctgggccgat
    10861 cgtggggtcg acgggttgcg cctggatgtg gtgaatctga tctccaaaga cccgcgtttc
    10921 cctgaagacc tggatggcga cgggcgtcgc ttctacaccg acgggccacg agcacacgag
    10981 tttttgcacg agatgaaccg cgatgtgttt acgccacgcg ggttaatgac cgtaggtgaa
    11041 atgtcctcca ccagccttga gcattgccag cgatacgcgg cactgacagg cagtgaattg
    11101 tcgatgacct ttaattttca tcacctgaag gtcgattatc ccggtggtga aaaatggacg
    11161 ctggctaaac ctgactttgt ggcgttgaaa acattgttcc gccactggca acaaggaatg
    11221 cacaacgtgg catggaatgc cttgttctgg tgtaaccacg atcagccgcg cattgtttct
    11281 cgctttggtg atgaaggtga ataccgcgtg cctgcggcaa aaatgctggc gatggtgctg
    11341 catggcatgc agggaacgcc gtatatctac cagggcgaag agattggcat gaccaacccg
    11401 catttcacgc gcattactga ctatcgcgac gtggagagcc tcaatatgtt tgccgagctg
    11461 cgcaacgatg gtcgtgatgc cgacgagtta ttggcaatcc tcgccagtaa atcccgtgac
    11521 aacagccgca cgcccatgca atggagcaac ggcgataatg ccgggtttac ggctggcgaa
    11581 ccgtggattg gcctgggcga taactatcaa caaatcaacg tagaagccgc gctggccgat
    11641 gactcctcgg tgttttacac ctaccaaaag ttaatcgcac tgcgtaagca agaagccatc
    11701 ctgacatggg gcaattacca ggatctgctg ccaaacagcc ctgtattgtg gtgctatcgc
    11761 cgtgaatgga aggggcaaac cttgctggtc attgccaacc ttagccgtga gatccaaccc
    11821 tggcagccag ggcaaatgcg cggcaactgg cagcttgtga tgcataacta cgaagaagcc
    11881 tcaccacaac cctgtgccat gaatttacgg ccttttgagg ctgtctggtg gttacagaag
    11941 taaatcttcc ctaagccccg gtaatgccgg tcattccggg gttttgtctg tcagcatgtt
    12001 gtttttgttg atttttcaac cagcaaattc attaaaaaat ttacataacg ctgtagcgcc
    12061 cgtcatccgt acgctctgct ttttactttg agctacatca aaaaaagtta aaacatcctt
    12121 gatgcaaagc actatatata gactttaaaa tgcgtcccaa cccaatatgt tgtattaatc
    12181 gactataatt gctactacag ctccccacga aaaaggtgcg gcgttgtgga taagcggatg
    12241 gcgattgcgg aaagcaccgg aaaacgaaac gaaaaaaccg gaaaacgcct ttcccaattt
    12301 ctgtggataa cctgttctta aaaatatgga gcgatcatga caccgcatgt gatgaaacga
    12361 gacggctgca aagtgccgtt taaatcagag cgcatcaaag aagcgattct gcgtgcagct
    12421 aaagcagcgg aagtcgatga tgccgattat tgcgccactg ttgccgcggt tgtcagcgag
    12481 cagatgcagg gccgcaacca ggtggatatc aacgagatcc agaccgcagt tgaaaaccag
    12541 ctgatgtcgg gtccatacaa acaactggct cgtgcttaca tcgagtaccg tcacgatcgc
    12601 gacattgaac gtgaaaaacg cggtcgcctg aaccaggaga tccgtggtct ggtcgagcag
    12661 accaacgcct cgttactcaa cgaaaacgcc aacaaagaca gcaaggtgat tccaacccag
    12721 cgcgacctgc tggccgggat cgtggctaaa cactatgcac gccagcacct gctgccgcgt
    12781 gacgtggtgc aggcacatga gcgtggcgat attcactatc acgatctcga ttactcgccg
    12841 ttcttcccga tgttcaactg catgttgatc gacctgaaag gcatgctgac ccagggcttt
    12901 aaaatgggga acgccgagat tgaaccgccg aagtcgatct ctacggcaac cgcggtaact
    12961 gcgcagatta ttgctcaggt cgccagccat atttatggcg gcaccaccat taaccgtatc
    13021 gatgaagtgc tggcgccgtt tgttaccgcc agctacaaca aacatcgcaa gaccgcagaa
    13081 gagtggaaca tcccggacgc cgaaggctac gctaactctc gtaccatcaa agagtgctac
    13141 gatgccttcc agtcgctgga gtacgaagta aacaccctgc acaccgccaa cggtcagacg
    13201 ccgtttgtaa cctttggttt tggtctgggc accagcaggg aatcgcgcct gattcaggaa
    13261 tcgatcctgc gtaaccgtat cgcaggcctc ggtaaaaacc gtaaaactgc ggtgttcccg
    13321 aaactggtgt ttgcgattcg cgatggcctg aaccataaaa aaggcgatcc gaactacgac
    13381 atcaaacagc tggcgctgga gtgcgcaagc aagcgcatgt atccggatat cctgaactac
    13441 gatcaggtag tgaaagtcac cggttcgttt aaaaccccga tgggctgccg cagcttcctc
    13501 ggcgtgtggg aaaatgaaaa cggcgagcag atccacgatg gtcgtaacaa cctcggcgtg
    13561 atcagcctga acctgccgcg tattgctctg gaagcaaaag gcgatgaagc caccttctgg
    13621 aagctgctgg atgaacgtct ggtgctggca cgtaaggcac tgatgacccg tatcgctcgt
    13681 ctcgaaggcg tgaaagcgcg cgtcgccccg atcctctata tggaaggtgc ttgtggcgta
    13741 cgtctgaatg ctgacgatga tgtttctgaa atcttcaaaa acggtcgtgc gtctatttcg
    13801 ctgggttaca tcggcatcca cgaaaccatt aacgcgctgt tcggcggcga gcatgtttac
    13861 gacaacgagc agcttcgcgc gaaaggtatc gccattgttg aacgtctgcg tcaggcagtg
    13921 gatcagtgga aagaagaaac gggttatggt ttcagtctct acagcacgcc gagtgaaaac
    13981 ctgtgcgacc gcttctgccg tctcgatact gctgagtttg gcgtggtgcc gggcgtgacc
    14041 gataaaggtt actacaccaa cagtttccac cttgatgtgg agaagaaggt gaacccgtac
    14101 gacaagatcg actttgaagc gccttacccg ccgctggcga acggtggttt catttgctac
    14161 ggcgagtatc caaacattca gcacaacctg aaagcgctgg aagatgtctg ggattacagc
    14221 tatcagcatg taccgtatta cggcaccaat acaccgattg atgagtgcta cgagtgtggc
    14281 tttaccggtg agttcgagtg caccagcaaa ggcttcactt gcccgaaatg tggtaaccat
    14341 gacgcctccc gtgtgtcggt aactcgccgc gtgtgcggat atttaggtag cccggatgcg
    14401 cgtccgttta acgccggtaa gcaggaagaa gttaagcgcc gcgttaaaca tttggggaat
    14461 gggcagatag gttaatcttc actctgcgtt aagcaaatgc cggatgcggc gtaaacgcct
    14521 tatccggcct acatttggca acggctgtag gcctgataag acgcgacagc gtcgcatcag
    14581 gcattgatta ccggatgcag cctacacgcg gactgataag ccaggcgaaa taatgaatta
    14641 tcatcagtac tatcctgtcg acatcgtcaa cggccccggc actcgctgca ccctgtttgt
    14701 ctccgggtgt gttcatgaat gccccggttg ctataacaaa agcacatggc gggtaaattc
    14761 tggtcagcca tttaccaaag caatggaaga caagatcatt aaagatctga atgacactcg
    14821 tatcaaacgc caggggattt ccctctccgg cggcgatccg ctgcatccgc aaaacgtgcc
    14881 ggatattctg aaactggtaa aacgcatccg cgccgagtgt ccgggtaaag acatctgggt
    14941 gtggacaggc tataaactcg acgaactcaa cgctgcgcaa atgcaggttg ttgatttgat
    15001 taacgtgctg gtcgacggca aatttgtgca ggatttaaaa gacccttccc tgatctggcg
    15061 cggcagcagc aatcaggtgg tgcatcattt gcgatgataa gttccctgtt acctgagtgc
    15121 aggcaacagg gaaatgagga attaacgata cttctggtga taggcgttgc gggtcgtttt
    15181 cagttgctct gcagcagcct gcgcttcttt tactttacct tcatttgcca gcttcagcgc
    15241 gtcgtcaatc tgaccgacca gaatgtcgaa accgtggcgg aaatctttca tttccgggct
    15301 gtccggtgat ttatcttcga gcttcggcgg cgttgctttt tgcgcatcca gcgctgcggc
    15361 gcgcatcttc gttaacgcgt ctttgacttg cgtcgcgtta tcggcttttt cgaccacttt
    15421 taaattgtcg ttgagggttt ccatattgtc ttcaagatca gcggcaaacg acgccgaact
    15481 gaataccaac gaggagactg caagaatagc taacaggctt ttacgcattg ctcacttcct
    15541 tttttattta cacttcatcc ttcacactgc ctcatcgaca gctacgctca ttcgaccggg
    15601 tcacgtacta cgagtacgct ccccggctct cattcacttt ccgtctcgat gcagtgcgaa
    15661 tgattttgtg taatttttta gtttatagcg cggcaggtcg cgccagtttt tttactgtcc
    15721 ggcgattttc atctccggca acagcacaga accacactgt atattactgc gtgtttcaat
    15781 atcgttaccg acggtgacaa tattgcgcca catatctttt aaattacctg cgatggtgat
    15841 ttcgctcacc ggatactgaa tttcgccatt ctctacccag aagcccgcag caccacggga
    15901 ataatcaccg gtaatggcac tcacgccctg gcccatcagc tccgtcacca ccagcccggt
    15961 gcccatctct ttgagcattt gttcgaagct tagcccttgt ccggcaatcc gccagttgtg
    16021 aataccgccc gcatgtccgg tgcttttcag cccaagtttt cgcgccgaat agctggtcag
    16081 cagccactga gtcaggatgc catctttaat aatatcgcga cgctcggtgc gcacaccttc
    16141 gctgtcgaat ggcgtcgacg ccagcccttt cagcagatgc ggatgctctt caatggtcag
    16201 ccagtccggc agaatttgtt tacccagcga atccagcagg aaggtagatt tacgataaac
    16261 cgatccaccc gctatcgccc ccaccagatg cccaaaaagc ccagttgcca cttcattggc
    16321 aaaaatgact ggcgctttca tggtggagag tttacgcggt aacaggcgcg ataaagtacg
    16381 gcgagcacag tcggccccaa cccactctgg cgtttgcaga tcgctcatcg cgcgcccaat
    16441 ggtgtaggcg taatcacgct ccatatcgcc attttcttcg gcaattacac agctggagag
    16501 cgaatgacgc gttgagcagt aaccctgcaa catgccgtgg ctgttgccaa aaactttgac
    16561 accgtagtgg ctgttaaagc tgccaccttc ggtattggtg atacgtttat ccgcctgtaa
    16621 tgctgcctgt tctgcgcggg ccgccagttc aatggcttca tccggggaaa cttccgcagg
    16681 gtggaacaag tcgagatctg gtgcgtcaaa ggccagcagc tctttgtctg ccaggccggc
    16741 acagggatct ggcgaggtat aacgggcaat atccagcgcc gcctgtacag tacgggcaat
    16801 ggcctgcggg cttaaatcgg tggatgatgc gctacctttg cggttctggt gataaacagt
    16861 gatccccagc gcgccatcgc tattgaattc aacattctcc acttcaccat aacgcgtgct
    16921 tacgctaatg ccggtggtct tgctgacggc aacttccgca ccgtccgatt tgcctgaggc
    16981 caactccagc gcagtggaaa ctgcttcttc cagaatcttg cgctgcgctt caacttgaga
    17041 gattactttc attgcaagtg ccataatgta gagagtttct ctgaagtcta acagagaacc
    17101 gtttttcagt gcgcatctta actggtaaca ttagcctctt tttttaagga gcctgagatg
    17161 actaagcagc ccgaagactg gctcgacgac gttcccggtg atgacatcga agacgaagac
    17221 gatgaaatta tctgggtcag taaaagtgaa attaaacgtg atgccgagga gctaaaacgc
    17281 cttggcgcgg aaattgttga tctggggaaa aacgcgctgg ataagatccc gttagatgcg
    17341 gatctgcgcg cggctattga gctcgcccag cgtattaaga tggaaggtcg ccgccgccag
    17401 ttgcagctca tcggtaagat gctgcgccag cgcgacgtag agcctattcg tcaggcgctg
    17461 gataagctga aaaaccgtca caaccagcag gtggtgctgt tccataaact ggaaaacctg
    17521 cgcgatcgtc tgatcgatca aggtgatgac gccatcgctg aagtattgaa tctgtggccc
    17581 gatgccgatc gtcagcaact gcgtacactg atccgcaacg cgaagaaaga gaaagaaggg
    17641 aataagccgc cgaaatccgc acgccagatt ttccagtatc tgcgcgaact ggcggagaat
    17701 gaagggtaat tttcgttgtt gatgtgagta tggtttgccg gatgcggcgt aaacgcctta
    17761 tccggcaatg ctatatcagg ctgaagccga attactgcgc agcttccgcc ttcttcgcca
    17821 gaccatccag cagtttctga tggatcccac caaaaccgcc gttgctcatc accagaatat
    17881 ggtcgccagg ctgagcggtt ttcaccacca tatctgccag cgtatccaca tcgccactcc
    17941 agtgtgcagg ctgaacgcag gcttctgcca cttctgccac ctgccacgga atatgcgccg
    18001 gttgcagcag gaagacttca tcggcacgac ctaatgaagg tgccagatcg tctttgcaga
    18061 tccccatttt catggtattc gagcgcggtt ccagcacagc aataatgcgc gccgtaccac
    18121 caactttgcc acgcagcgcc gccagcgttg ccagaatcgc cgtcgggtga tgggcaaaat
    18181 cgtcatatac cgtgacgcca ttcgcttcac cacgcaactc cagacggcga cgagcattaa
    18241 taaacgaacc cagcgcgtta gcggcatctg ccgacgctac accaacatgg cgagccgctg
    18301 caatcgccat caggccattg tgcatattat gttcgcctac cagcgaccat ttcacttcgc
    18361 ccactttttc gccatccagc aaaacttccc attcggaagc atcggtggtc agctttttcg
    18421 cctgccagtg accctgctca cccaccagct cctgctcgct ccagcagccc atcgccatgg
    18481 tctgtttcag gttgatgtca ttttccggcc agataatacg gccctgcccc ggaacgatac
    18541 gcaccagatg gtggaactgt ttctggatcg ctttcaggtc gtcaaagata tcggcgtgat
    18601 cgaactcaag gttgttgagg atcagcgtac gcgggcagta atggacaaat ttagagcgtt
    18661 tgtcgaagaa ggcgcagtcg tactcatccg cttcgataac aaagaagtcg ctttcgccca
    18721 gacgcgccga aacctcaaag ttccccggca caccgccgat cacaaatccc ggtttgtaac
    18781 cacactgttc cagaatccag gtcgccattc ccgcggtggt ggttttgccg tgtgtaccgg
    18841 caacggccag cacccagcgg tcgcgcagca caaaatcgtg cagccactgt ggacctgaca
    18901 tataagggat gtttttttcc agtaccgctt ccacacacgg atttccacgg gtcatggcgt
    18961 tgccaataat caccagatcc ggctgcggct cgagctggct ggcatcgtaa ccctgaatca
    19021 gctcaatgcc ttgcttctca agtaaggtgc tcatcggcgg atacacattg gcgtccgaac
    19081 ccgttacttc atggcctaac tggcgcgcca gcatcgccag accgcccata aatgtgccac
    19141 aaattcctaa aatatgaatg cgcatacgtc actatccttt tttaatctgc cgctcatttt
    19201 acgcatatgt cgggcaagtg agaaacgcat ttcaggataa tccgcaattt gctggcgcga
    19261 ttcacctgag cgcaacattg agattatttg ttaagattgt tgcggtcgct ttactccata
    19321 aacattgcag ggaaagtttt atgaaaacgt taggtgaatt tattgtcgaa aagcagcacg
    19381 agttttctca tgctaccggt gagctcactg ctttgctgtc ggcaataaaa ctgggcgcca
    19441 agattatcca tcgcgatatc aacaaagcag gactggttga tatcctgggt gccagcggtg
    19501 ctgagaacgc gcagggcgag gttcagcaaa aactcgactt gttcgctaat gaaaaactga
    19561 aagccgcact gaaagcacgc gatatcgttg cggggattgc ctctgaagaa gaagatgaga
    19621 ttgtcgtctt tgaaggctgt gaatacgcaa aatacgtggt gctgatggac cccctggatg
    19681 gctcgtccaa catcgatgtt aacgtctctg tcggtaccat tttctccatc taccgccgcg
    19741 ttacgcctgt tggcacgccg gtaacggaag aagatttcct ccagcctggt aacaaacagg
    19801 ttgcggcagg ttacgtggta tacggctcct ctaccatgct ggtttacacc accggatgcg
    19861 gtgttcacgc ctttacttac gatccttcgc tgggcgtttt ctgcctgtgc caggaacgga
    19921 tgcgcttccc ggagaaaggc aaaacctact ccatcaacga aggaaactac attaagtttc
    19981 cgaacggggt gaagaagtac attaaattct gccaggaaga agataaatcc accaatcgcc
    20041 cgtatacctc gcgttatatc ggttcactgg ttgcggattt ccaccgtaac ctgctgaaag
    20101 gcggtattta tctctaccca agcaccgcca gccacccgga cggcaaactg cgtttgcttt
    20161 atgagtgcaa cccgatggca ttcctggcgg aacaagcggg cggtaaagcg agcgatggca
    20221 aagagcgtat tctggatatc atcccggaaa ccctgcacca gcgccgttcg ttctttgtcg
    20281 gcaacgacca tatggttgaa gatgtcgaac gctttatccg tgagttcccg gacgcgtaat
    20341 ctcctgcgcc tctccacacg gagaggcgtt tttattgctg cgcaatgttg actcttgtca
    20401 ctggcgaact ctgacgattc tcccacagca ccgttaatcc acgctgtaat gctataaaaa
    20461 taaacaacaa aataccgatg gcgattttcg tccaccagga actcagcgtg ccatcaaagt
    20521 ttatgtaagt ctgaatcagt ccctgaatcg ccacgccaaa aagcgtccct aataccgttc
    20581 caacgccacc gctcaaaagc gtaccgccaa ttaccactga ggcgatagca tccagctcca
    20641 cacctacgcc cgccagcgca tatccggcct gggtataaat cgagaagaca atccccgcca
    20701 gcgttgccag tccggtggag agcatataaa tgcgaatagt ggtgctgcga gtggaaatcc
    20761 ccatcagatt cgccgacgtt gcgttgccgc caatggcgta tacctgatta ccaaaacggg
    20821 tacgatgcgc gaggaatatg ccgataacca ccaccgccaa catcagcagt cccatcgcac
    20881 ttaagcgacc gccgccaggg attttccacg caaggcttga gagcgtgtca taaatgggat
    20941 ggtttatcgg aatcgactct tccgaaacga gatagctgac gccgcgcaaa aagaacatcc
    21001 ccgcaagcgt aatgatgaac gccgggatct tcagggcgtc gatcagaagc cccataaatg
    21061 cgccgaaggc acagcccatc accagcacca gcggaaacgc cagcagcggc gagaggccga
    21121 aatcgccaat cacttttgcc agaaacacgc cggtaaaggc gatcaccgaa ccgacggaga
    21181 gatcgatccc accggagagg atcacaaagg tcatgccaac ggcaatgatc ccaagaaagg
    21241 cgttatcggt caggatattg cagatcactc ttgtggaagc aaaaccagga aactgcgtca
    21301 ggcagtagag atagcccagc acaaagacgc cgatggtgat cataagcggc aagttacgtt
    21361 ttatcacggc tacgcactcc tttaatcaga ctgataaagc gttgcgactg aacaatcagc
    21421 acacaaagca ccaccaccgc tttcaccacc tggttcatct ctggcggaaa gcccgaaagc
    21481 aaaattccgg tattcattcc ctgaataatc agcgccccaa ctcccgaaag cagcaggtta
    21541 aagcgaccgc ccatcagcga tccaccgcca atcaccaccg cgagaatggc atccagctcc
    21601 agccataatc cggcgttgtt ggcatcggca ccgcgaatat ccgccgccac gataatgccc
    21661 gcaatcgccg cacatagccc gctcaacacg taggtgagca tgacgatgat tcgtgtgttg
    21721 actccggcat tttttgccgc ccgaatgttg ataccaacgg cttcgataaa catccccagc
    21781 gccgttttgc gggtcaacag ccagaacagg agaagcgtca gcaccgcgat aatgaccggc
    21841 gttggcagga acaacagcga tccgctgcca aaccatgaga gatccggcga gttaaacgtg
    21901 acgatctgcc cggcggtgat cagttgcgcc acgccgcgcc cggcgaccat caggatcagg
    21961 gtggcgacaa acggctgaat tttgaggatc gctaccagta tgccgttcca caatcccgct
    22021 aggatgccgg tgcccagggc gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc
    22081 atcgcagccg ttgtggctcc ggcgatagcc atcaccgccc ccacagagag atcgatacca
    22141 ccggtggcga tcaccagcgt cataccaatc gccagtaacg caacgggagc tgcacggtta
    22201 agaatgtcga tagggctacc gaacaaacgc ccatcctgaa gcaccacctg ccagaaatgc
    22261 ggggctacca ggctatcgac cagcagcacc agcaacagtg ccgccagctg cggcatcccc
    22321 gtcggccagt gaaaacgcct ttttggcggc gtagtgtcag ggagagattg aggcatcaca
    22381 ctgttctcct tacgccgcaa tggcgttcat gatcgccgga acggaaagct gtgccagcgg
    22441 gatctccgcc acctgtttgc gatcgcgcat gatgatcacc cggtcggcat agcccaccag
    22501 ctcttccagt tcggaggaga tcaccagcag cgccagaccg tcagcacaca gcgtttcaat
    22561 caggcggatg atctcggcgt gggcaccaac atcaatgcca cgggttggct catcaaggat
    22621 cagaaattgc ggtcgggtca gtagccaacg tgaaagcagc actttttgct gattgccacc
    22681 ggagagaaat tcaatcggtt gttcagttga aggtgtgcga atgccaagct ggcggataaa
    22741 gcgttcggca atctcttgct gctctttgcg ggaaatgggt cgtagccagc cgcgctgggc
    22801 ctggagagcg aggatgatat tttcccgcac cgaggcggcg gcgatgatgc catcggtttt
    22861 cctgtcttcc gggcagaatc cgatgcccag tactgaagcc tgatgtggcg atcgcagggt
    22921 ttgcggtttg cctttgatca acgccgtgcc gctgtcagca ggtttgatac cgaagatcac
    22981 ttcggcggtt tcggtacgtc cggatcccag caagccagcc agaccgacga tctcgccggg
    23041 gcgcacttcg agatcaaacg gtgctatcgt tccttttttg ccgtaatttt tgaacgcggc
    23101 aacgggtttg tcgctcaaca atgttcgccc ggcacgctgt agcacgtggg tatccagctc
    23161 gcgccccagc atcattttta ccagttcgat ctgcggtagc tcgcgcgttt cccgacagcc
    23221 tacgaaactg ccgttgcgta agacagtgat ccgatcgctg acctgataga cctgatcgag
    23281 aaagtgagtg acaaagatca ggctgacgcc gcgatcgcgc aactgacgca tcaggccaaa
    23341 taatagctcc acttcctggg tgtcgaggct ggcggtgggt tcatcgagga tcagcacttt
    23401 ggcggagaga tcgatagccc ggcaaatggc gacgatttgc tgcatcgcga ctgaaaagcg
    23461 gttaagcggt tcgcgcacgt cgaggaagaa accgtaagat gccatcagtt cggtggcgcg
    23521 cttttccatc tctttacggc gtagaaggcc gaaacgtttg ggttcgcgac ctatgaatag
    23581 attatcagca accgacatat tgggtagcag gttgacttcc tgatagacgg tgccaatacc
    23641 gagttgttgt gcatgggcgg tatttttcgg tgagatagcc tggccttcca gccagatggt
    23701 gccgcgatcg gcgtggtata caccagttaa tgctttgatt agcgttgatt ttcccgcccc
    23761 gttttcacca agcagcgcca tgatttcgcc acggcgcagg ctgaaatcaa cgttgtctaa
    23821 cgctttgacg ccggggaaaa atttacttaa tccttcggtg cggaggatct cctggtgttg
    23881 gtcggtcgtc atggtttccc ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg
    23941 gtaagcgtcg catcaggctt cagtgcacca tcgccggatg cggcgcgagc gccttatccg
    24001 gcctacgaat cggcatcagg catagcaacc ctcaataccc catatttttc ttcttctcta
    24061 actcttcttt tgcggtatca ggcaggtaaa gggtggattt ggttaacgtc agcttttcag
    24121 gcatggtgcc gtcttttttg tatttctcca gcgcatcgaa agcgggtccc gccatgttcg
    24181 gcgtcagttc aacactggcg ttcgcttcgc catccatcat cgctttgtag atgtccggta
    24241 caccgtcaat ggaacccgtg aggatatctt tgcccggttt caggcccgct tctttaattg
    24301 cctgaattgc accaatcacc atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc
    24361 cgttgttttc cgctttgata aagctctcca tgacttcttt gcctttactg cgggtgaagt
    24421 cacctgactg cgagcggata attttgatat ttggcgcatt cttaatggct tcggcaaagc
    24481 ctttcttacg gtcaatggcg acgctggccc caacggtgcc ctgcagctcc accacgttgc
    24541 atggtttgcc attcacttct tttaccagcc agtcaccgat caacttacct tcgaggatgt
    24601 tgtcggcagt aacggtggtc atatagagag atttgtcttt cacatcgatg gaacggtcga
    24661 gcaagaagac cgggatttcg gcatctttcg cctcttttaa taccggttcc caacccgtcg
    24721 caaccactgg agcgataaag atcgcatcca ccccttgtgc aacgaaggaa cgtaccgctt
    24781 taatctggtt ttcctgcttt tgctgaccat cggcaatttt caacgtaatt ccgcgttttt
    24841 cggcctcact tttcgccaca ttggtttctg cggcgcgcca gccggattcc gatccgacct
    24901 gcgaaaatcc tccggttaat ggagctgcca acgccataga cgacatggct gccgagactg
    24961 cagagactac aagtaagcgt ttccacatag ggttgtcctc gtcggggtaa ttattattgg
    25021 tgaaaagatg ttcgcgaaaa aactatagac aattcgttat gtaacggatt gcgttacatc
    25081 acacttcaaa aaagtaaata aaacgttaat cacaagtttg taatcgcttt catctcacta
    25141 tgaaaaatgc ggctacggtt atggattttc ctgctctgta taccgtctta aaactggcga
    25201 aaaaggaaaa tgaagacgaa aacaagcaaa gacattcggc gcgagttggc tataatactc
    25261 ggcacttgtt tgccacatat ttttaaagga aacagacatg agcttactca acgtccctgc
    25321 gggtaaagat ctgccggaag acatctacgt tgttattgag atcccggcta acgcagatcc
    25381 gatcaaatac gaaatcgaca aagagagcgg cgcactgttc gttgaccgct tcatgtccac
    25441 cgcgatgttc tatccgtgca actacggtta catcaaccac accctgtctc tggacggtga
    25501 cccggttgac gtactggtcc cgactccgta cccgctgcag cctggttctg tgatccgttg
    25561 ccgtccggtt ggcgttctga aaatgaccga cgaagccggt gaagatgcga aactggttgc
    25621 ggttccgcac agcaagctga gcaaagaata cgatcacatt aaagacgtaa acgatctgcc
    25681 tgaactgctg aaagcgcaaa tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg
    25741 caagtgggtg aaagttgaag gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc
    25801 ttccttcgag cgcgcaaaga ataaataagt tcttctagcg caataaccct gaacgccggg
    25861 cttcggttag taagggtttt ttatgcccgc gataaataaa ctctctattc caccatcatt
    25921 attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct
    25981 gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc
    26041 ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg attgcagata
    26101 tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga
    26161 tacatcgcat aaaacaacgc cacccgaagg tggcgtgctt ttattattac ttatccctgt
    26221 ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca
    26281 tccatgcact cccgtacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc
    26341 gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg
    26401 ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga
    26461 tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc
    26521 gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag
    26581 tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa
    26641 cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa
    26701 tacatagccg ctgaccacta cctgggagga gtcgcctact ccctgggtgt cgagcgctaa
    26761 attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc
    26821 cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg
    26881 tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc
    26941 cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcaattacgt cgtcttctgt
    27001 agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg gaccagagaa
    27061 cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg
    27121 gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt taagatcgcc
    27181 cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat
    27241 caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt
    27301 aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat
    27361 gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa
    27421 tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact
    27481 gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt
    27541 caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt
    27601 aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc
    27661 cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg
    27721 caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat
    27781 cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc
    27841 ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa
    27901 ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag
    27961 cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc
    28021 ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc
    28081 gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga agcggttgag
    28141 attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc
    28201 attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc
    28261 cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaaac gggtattgct
    28321 aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc
    28381 ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc
    28441 attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc
    28501 gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac
    28561 ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc
    28621 caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt
    28681 atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc
    28741 tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca tccactgcat
    28801 ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt
    28861 cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt
    28921 aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt
    28981 aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc
    29041 agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc
    29101 tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat
    29161 agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata
    29221 ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagtgg
    29281 caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag
    29341 gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct tcaccttttc
    29401 acctttcaac ggctccacgt tcagtgtgct gttgaggatg atatccaccg gccagttgtc
    29461 tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc
    29521 gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat
    29581 gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag
    29641 cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt
    29701 ttcgccgagc ggcttttcat ccggctgcgg attttcaatc ttcggttcga cgacctcttc
    29761 ctgcgccact tccgccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt
    29821 cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt
    29881 ggtgtcatca atcttgatgt tgacgttatc cagcgccacc cgtgtcaggg tgatgggata
    29941 cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg
    30001 cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac
    30061 gctactgttc cacaggcact caagaccgac cgccagatgc agattacccg cttttaccgc
    30121 cacgcctggc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac
    30181 gttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc
    30241 gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac
    30301 gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt
    30361 aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc
    30421 ggcgattccc agcgtacgcc gcccccggta ccggttttaa agtcgctgcg gcgaatatcg
    30481 cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac
    30541 tggtattcca gcgatccggt tatcagcttc gaggcccctt tcaggtcacc gttggcgtat
    30601 ttcggggcga tagatttata tttgtagccg cgaatactac gatcgcctcc ggcgaagaaa
    30661 cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt
    30721 gtcacaaaac gatggcgatc gtacagtgtg cggatccaga cgttctgcgc ctggaaaacg
    30781 gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag
    30841 tcgccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga
    30901 taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag gctccagcgc
    30961 aggttaatgg cacgctgcca gtcgctggag agatcccagt agcgagaagc caccagcgta
    31021 gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa
    31081 tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag gatctgttcc
    31141 ggcgcggaaa tactggtact ggtggtcaga ctgtgaccat aagagttcat ccacggcttt
    31201 ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg
    31261 gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct
    31321 ttatcaaatt gcggtgccac taccaccgaa ttaaaccagc cggtagcaga aagtcggcgg
    31381 ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg caccagattt
    31441 tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt
    31501 tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc
    31561 tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac
    31621 tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg
    31681 agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg
    31741 ccaattaaca ccggtacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt
    31801 ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct
    31861 tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt
    31921 tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc
    31981 tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat
    32041 aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctacc cctggaacgc
    32101 cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat
    32161 atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt
    32221 gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat
    32281 aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta
    32341 gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg aatggagatt
    32401 gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc
    32461 gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa
    32521 gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc
    32581 atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg
    32641 cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct gaccccagaa
    32701 caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat
    32761 gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat
    32821 gaccaccagc aatatctgca taaaaaccca tatggttact gtggaattgg cggaattggc
    32881 gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc
    32941 tctttgtcta tgcctgatgc ggcttaagcg acttaccagg cctacaaaat cgtccaaatt
    33001 caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt
    33061 tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc tatactagcc
    33121 actgaaaatg tcggttcact ttcttcgaat cgactttcaa tgtgtatttc acacaaatta
    33181 atcaactttc ccttccgagg atctggcctg aaaggtcgga taagatatgt taaacagtat
    33241 tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct
    33301 tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca
    33361 acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct
    33421 gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca
    33481 cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc
    33541 tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg
    33601 tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta
    33661 cgtttgcacc ccactggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt
    33721 caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga
    33781 agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt
    33841 tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt
    33901 tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa
    33961 gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct
    34021 gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa
    34081 cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc
    34141 aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct
    34201 caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat
    34261 tgtcgcccgt gatgagaact catggctgat tgacggcggc actccgattg acgacgtcat
    34321 gcgcgtgctg gatattgacg aattcccgca gtcgggcaac tacgaaacca tcggcggctt
    34381 tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta
    34441 caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat
    34501 cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag
    34561 cgtcgcgtaa ccccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc
    34621 atagcacttt ttactgcata gcacttttta ctgcatagca ctttggttaa gccatctctg
    34681 tttgcagacg cataacctga cggttaactt cggacatcac tgacaaatgc agcttatcct
    34741 tcacttttgg gataagaatc ttacccttat caaattcaaa agcgccaacg tccttgatgt
    34801 ataaccgtcc acggaacagg atctttacgt acttcgccac ctgaagtggg ttgtaccgtt
    34861 ggaaaatttt cattgttatc tcctgctgag tattacgcct gtgcggtgcc acaatcggcc
    34921 caactattat gaggcgcaaa ttttaatgct tagtgactat agactatccg ggcaatgttt
    34981 ccaccgcgta taactttttt tacctaaagg ttacaattat tcagaattat ctttttaccg
    35041 aagcgcggtc ttcagtataa gcattcattt ttcatatgat ttgtgcgctt gaccgcaaac
    35101 tggcatcaca cttgcgggaa attcgataaa tagcacatat gattaaaact cagacccaag
    35161 tggtcggatc acctgcatat cataagaagg aaacaccatg accctacgca agattctggc
    35221 actcacctgc ctgctgttgc cgatgatggc ttccgcacat cagttcgaaa ccggtcagcg
    35281 agtgccgccg attggcatta ccgatcgggg cgagttggtg cttgataaag atcagtttag
    35341 ctacaaaacc tggaacagcg cgcagttagt gggaaaagtg cgagtactgc aacatattgc
    35401 tggtcgcacc tctgcaaaag agaaaaacgc gacgctgatt gaagcgatta aatcagcgaa
    35461 gttaccgcac gatcgttacc agaccaccac tattgttaac accgacgacg cgattccggg
    35521 ttcagggatg tttgtgcgca gcagtctgga gagtaataaa aagctttatc cctggtcgca
    35581 gtttattgtc gatagcaatg gcgtcgcgcg cggtgcctgg cagctggatg aagaaagttc
    35641 cgctgtagtg gtgctggata aggatggtcg cgtgcaatgg gccaaagacg gggcgctcac
    35701 tcaggaagag gtgcagcaag tgatggacct gctgcaaaag ttgctcaaat aatggcaaca
    35761 acatagattc tgcaaaaata attttcccct atcgttatct atgggatagg ggaattaagt
    35821 ctctgagttc ttacgccgaa ctcatactgg taacgctagt tgctcttttt taaacgcctc
    35881 aatggtcgac ttatatttct cgcaaatctt ttctttggta atattactat ctgcggccag
    35941 attcgcactt actatcgcat ccacttgttc ggctaattcc acttgttggc aacctgcatt
    36001 aaccagaaaa ttataaataa gggagtaatc ttcatcctcc aaaacaacag aggaaccatt
    36061 acgaaaacgt tcatcccctc catagatttt aagcgcaaag cccaaagatt ctatgactat
    36121 ggcaaaatta tcatcaatta ttttttcgtg aatataccat ccatcgcatt ctttaattcc
    36181 acctcctggt atttggagtt caaatttacc ccgttttaat ttgcttacta tatcctccaa
    36241 taaggcataa cctaaagaca cgtcaccaat cgcctggtga atataacctt taatcctttt
    36301 cggtatgtca gactgttttt tctgcagaat atcacaggca tacaatcttc tgttttgttc
    36361 taacggaaac tcgcggtttt tgaaatccat gaatgagtta tactcattta ttgttatggg
    36421 aatttcttcg tattcaaatc cactacctcg caatgtcatt gtatattcgg caccgttatc
    36481 tttaaggctg aaactgataa ggaacttatc catggcgtta acagttccat tttttgatga
    36541 aagtacctga taactatttt tatcaatctg agttgcgcaa cgaaagaact ccctcaaagt
    36601 taacaggcta tcataattcg aaatccaatc ttttttctta aatgcatgct ggatattgtt
    36661 aaaaaaattg tttgaatgtg tttttgtata cgatgacttt acaaataact ccaccgcacc
    36721 aataaaatta ttgtgtctga tcctgggtaa catatgacaa ccttatttta tttggttatt
    36781 ttatgtcgcc attaaatatc tcattagaga agtaaactat caaaaaacgc tcattattaa
    36841 aatgtaactt attagcaatt gaatttttgc actttaaata caatctcttt ttatttataa
    36901 ctttttaggt tgggagaaat caggatggca gaatcaggaa atacactgtt tctgccatct
    36961 gaatttagta aatagacact ctgaaccccg gattcaggaa cgactcgcgc ggagtgtaat
    37021 ccagcggttt accctgccag tcgtgaacgt gcgctccggc agctgcagcc acagcatgtc
    37081 cagcggcggt gtcccaaata ttcgttggtc cgaagcgcgg gtacagctgc gcctgtcctt
    37141 ccgccaccag gcagaatttc agcgaagagc cgatggacgt ggtctgatgt tcgccaagct
    37201 gttgcagata ctctttcagc tccgcatctg catgggaacg gctgataacc accagcggcg
    37261 ggcgcgcatc gcggacctga atctgcttgc gcacaccgca ctcttctttc caggccttgc
    37321 cttctgccgc gctgtacatt acgttcatta ccggcgcata caccacacct aaaatcggtt
    37381 tgccatggtc aatgagcgca atgttaacgg tgaattcgcc attacgttta ataaactctt
    37441 tagtaccatc cagcgggtct accagccagt aacgctgcca gtgctgacgg acttcccaac
    37501 cgggaggatc ttcttcagaa aggaccggaa tatccggtgt cagcgtacgt aaaccgtcca
    37561 tgataacggt gtgtgcggca atatccgctg ccgttaccgg agaattgtcc gctttgctga
    37621 cgacgtccat cggtttcgtc ccgtcgtaga cctgcataat ggcatcgcct gcattccgtg
    37681 caagctggca tacttgatct aacatttctc cacctcgtct ctgtgagcgg tgttaactta
    37741 ttgttttact tataccctat cgttaatgaa tgcgccaact gtgatagtgt catcattttc
    37801 aaagcgtaaa attgtggcat tcttcactgt tctataagta agacgtttat tctccctttt
    37861 ctttcgtatt cccgatgata aaaggatgtc cctgatgatt aagtttagcg caacgctcct
    37921 ggccacgctg attgccgcca gtgtgaatgc agcgacggtc gatctgcgta tcatggaaac
    37981 cactgatctg catagcaaca tgatggattt cgattattac aaagacaccg ccacggaaaa
    38041 attcggactg gtacgtacgg caagcctgat taacgatgcc cgcaatgaag tgaaaaacag
    38101 cgtactggtc gataacggcg atttgattca ggggagtccg ctggcagatt acatgtcggc
    38161 gaaaggatta aaaacaggtg atgttcatcc ggtttataag gcgctgaata cgctggatta
    38221 tacggtcggt acactcggca atcatgaatt taactacggt ctggattacc tgaaaaatgc
    38281 gctggcggga gcgaaattcc cttatgtaaa tgccaacgtc attgacgcca gaaccaaaca
    38341 gccaatgttt acaccgtatt taattaaaga taccgaagtg gtcgataaag acggaaaaaa
    38401 acagacgctg aagattggct atattggcgt cgtgccgccg caaatcatgg gctgggataa
    38461 agctaattta tccgggaaag tgacggtgaa tgatattacc gaaaccgtgc gcaaatacgt
    38521 gcctgaaatg cgcgagaaag gtgccgatgt cgttgtcgtt ctggcacatt ccgggctgtc
    38581 tgccgatccg tataaagtga tggcggaaaa ctcagtttat tacctcagtg aaattccggg
    38641 cgttaacgcc attatgtttg gtcatgctca cgccgttttc ccgggtaaag attttgctga
    38701 tatcgaaggg gctgatatca ccaaaggcac gctgaatggt gttccggcgg taatgccggg
    38761 catgtggggc gatcatcttg gggtggtcga cttacaactc agtaatgaca gcggtaaatg
    38821 gcaggtgacg caggcgaaag cggaagctcg gccgatttac gacatcgcca ataaaaaatc
    38881 cctcgcggcg gaagacagca agctggtaga aacactcaaa gccgatcacg atgccacacg
    38941 ccagttcgtc agcaagccaa tcggtaaatc tgccgacaat atgtatagct atctggcgct
    39001 ggtgcaggac gatccgaccg tgcaagtcgt gaacaacgcg caaaaagcgt atgtcgaaca
    39061 ttacattcag ggcgatccgg atctggcaaa actgccggtg ctttcagctg ccgcaccgtt
    39121 taaagttggt ggtcgcaaaa atgacccggc aagctatgtg gaggtggaaa aaggccagtt
    39181 gaccttccgt aatgccgccg atctttatct ctaccccaat acgctgattg tagtgaaagc
    39241 cagcggtaaa gaggtgaaag agtggctgga gtgctccgcc ggacagttta accagattga
    39301 tcctaacagc acgaaaccac agtcactcat caactgggat ggtttccgca cttataactt
    39361 tgatgttatt gatggtgtga attatcagat tgatgttacc cagcccgccc gttatgacgg
    39421 cgagtgccag atgattaata ccaatgcgga aaggattaag aacctgacct ttaatggcaa
    39481 gccgattgat ccgaacgcca tgttcctcgt tgccaccaat aactatcgcg cttacggcgg
    39541 caaatttgcc ggtacgggcg acagccatat cgcttttgct tcaccggatg agaaccgctc
    39601 ggtgctggca gcgtggattg ctgatgagtc gaaacgtgcg ggggaaattc acccggcggc
    39661 agataacaac tggcgtttag caccgatagc tgccgataag aaactggata tccgtttcga
    39721 aacttccccg tcaaataaag ccgcagcgtt tattaaagag aaagggcagt atccgatgaa
    39781 taaagtcgcg accgatgata tcgggtttgc gatttatcag gtggatttga gtaagtaaaa
    39841 cacttctttt tcggcctata aatcatcaat cgcatccggc atttattggc ggatgcgatg
    39901 ctggcgcatc ttatccgccc tacaagtcat acaccgtagg ccgcgcatcc ggcagttatg
    39961 ccgcacgttc atcccgcacc gccaacacct cgggcaaatt caactcaatc cagtccgcca
    40021 gtgcagcaac cttttcgctc acctgctcgc ccagcggcgt gaggctgtat tcaacatgcg
    40081 gtggcaccac cggatacgcg atacggttaa gaaaaccatc ctgttctaac gcctgtaacg
    40141 actgcgcaag catcttttcg ctcaccccgc cgattttgcg ccgcaggtcg ctaaagcgat
    40201 gagtaccttc gcgtagcgcc accagaatca acacccccca acggctggtg acgtgtttca
    40261 acacctcgcg cgacgggcac tgttccgcaa agagattacc ctctttcagt tgttgcgaca
    40321 ggctaacctg actcatttca tacttacctt tttgtacgta cttactaaaa gtaagtttag
    40381 gtgttagcgt atttaaacac aagacaaaac gatggagact tcccatgatc gctattactg
    40441 gtgccactgg ccaacttggt cactatatta ttgaatcctt gatgaaaacg gttcctgcca
    40501 gccaaatagt ggctatcgtt cgtaatccgg caaaagccca ggctctagca gcacaaggca
    40561 ttaccgtgcg tcaggctgac tacggcgatg aagccgcact gacatctgca cttcagggag
    40621 tggaaaaact actgctgatc tcttccagcg aagtgggtca acgtgccccg cagcatcgta
    40681 atgttattaa tgccgcaaag gcggctggcg tgaaatttat cgcttatacc agcctgctac
    40741 atgcagatac ctccccgctc ggcctcgccg atgagcacat tgagacggag aaaatgctgg
    40801 ctgattctgg catcgtttac accctgctgc gcaacggctg gtacagcgaa aactacctcg
    40861 ccagcgcccc ggcagcactg gaacacggcg tatttatcgg tgcggcgggc gatggcaaaa
    40921 tcgcctcagc aacgcgggca gattatgcgg cagctgcggc acgcgtgatt agcgaagccg
    40981 gtcacgaagg caaggtttac gaactggcgg gcgatagtgc ctggacgttg acacagttag
    41041 cggcagagct gaccaaacag agcggcaaac aggttaccta tcaaaatctg agcgaagccg
    41101 atttcgccgc ggcactgaaa agcgtcggac tgcccgacgg actggcggat atgctggcgg
    41161 attctgacgt tggcgcatcg aaaggtggtc tgtttgataa cagcaaaacg cttagcaaat
    41221 tgattggccg cccaacgaca acgttagccg aaagcgtaag ccatcttttt aatgttaata
    41281 actagttaat taaagtggca tcctcccgca tcctctctga taatgacggg atgccgggag
    41341 caatcatgtc tgcttcctga actttcttct gacagaccaa tggatgccag taatgattag
    41401 cggcgtgctg tacgccctgt tagcagggtt gatgtggggg cttatttttg tcgggccgtt
    41461 gatcgtaccg gaatacccgg cgatgttgca gtcgatgggg cgttatctgg cgttagggtt
    41521 aattgcgctg cccattgcct ggctgggacg cgtgcgtctg cgtcagttgg cgcgtcgcga
    41581 ctggcttacc gccttgatgc tcaccatgat gggcaacctt atctattact tctgccttgc
    41641 cagtgccatt caacgtactg gcgcgcctgt ttccacgatg attatcggca ccctgccggt
    41701 ggtgattccc gtttttgcca atctgcttta tagccagcgc gacggcaaac tcgcatgggg
    41761 aaaactcgcc ccggcactgg tttgtattgg catcggcctg gcgtgtgtga atattgctga
    41821 gttaaaccac ggactccccg attttgactg ggcacgttat acctctggca tcgtgctggc
    41881 gttagtttcc gtggtctgct gggcgtggta tgccctgcgc aacgcccgct ggctgcggga
    41941 aaatcccgac aaacatccga tgatgtgggc aacggcgcag gcgctggtca cactgccggt
    42001 ttctctcatc ggctatctcg tcgcctgtta ctggctgaat atacaaacgc cggacttctc
    42061 cttacctttt ggcccccgtc cgctggtgtt tattagtctg atggttgcga tagccgtgct
    42121 ttgctcatgg gttggcgcac tctgctggaa cgtcgccagc cagcgattac cgacagtgat
    42181 tctcgggccg ctgattgttt tcgaaacgct ggcaggtttg ctgtacacct ttttactccg
    42241 ccagcaaatg ccgccgctaa tgacgctgag cggtatcgcg ctgttagtga ttggcgtggt
    42301 cattgcggtc agagcaaaac cagaaaaacc tttaactgaa tctgtctcag aaagttgaca
    42361 cgctggcagt gagttaaata agcctctgct acgtaagggt tatagctttt gccttaaaga
    42421 tgcatttaaa atacatctta tattattaag aatgaggtat cagctatggc ttatcgcgac
    42481 caacctttag gtgaactggc gctctctatt cctcgcgctt cagctctgtt tcgtaaatat
    42541 gatatggatt actgctgtgg cggtaagcaa acgctggcgc gcgcggcggc acgtaaagaa
    42601 ctggatgttg aggtcattga agctgaactg gcaaagcttg ctgaacaacc gattgagaaa
    42661 gactggcgta gcgccccgct ggcagaaatc attgaccata tcatcgtgcg ctaccacgat
    42721 cgtcaccgcg agcaactgcc ggagctgatc ctgcaagcga ctaaagtcga gcgcgttcac
    42781 gccgacaaac cgagcgtgcc aaaagggctg acaaaatacc tgactatgct gcatgaagag
    42841 ctatccagcc acatgatgaa agaagagcag atcctcttcc cgatgatcaa acaaggcatg
    42901 ggcagccagg caatgggacc aatcagcgtg atggaaagcg agcacgatga agcgggcgaa
    42961 ctgctggaag tgattaaaca caccaccaat aacgtcacac cgccgccgga agcctgcacc
    43021 acctggaaag cgatgtataa cggtattaat gaactgattg atgacctgat ggatcacatc
    43081 agcctggaaa acaatgtact gttcccacgc gcgctggcgg gtgagtaatc taaaagctgg
    43141 atggcattgc gccatccagc atgataatgc ggttatttcc gcagttcagc agcccgcttc
    43201 ttaccaataa acaaccagcc cagccccagt gcgataaacc acagcggggt gaccaacagc
    43261 gcctggcgag tgtcatcttc cagtgtcagc aacacgacca cgaacacaaa gaacgccata
    43321 catacccagc acatcagctt gccgagcggc atcttgtaga tcgacttctc atgcagatga
    43381 ggacgctgtt tgcggtaaac caggtacgag caaaggataa ttgtccagac gaacataaac
    43441 agaatcgcgg aaacggttgt aatcatcgtg aacgcgccaa tcacgctagg attcacatac
    43501 aacatcacca cgccgccgag cagacagata cacgagaagg tcaacccttt cgcgggtact
    43561 gcgcgcttag aaagtttagc gaacgctttc ggtgccacac cttcctgcgc cagaccaaac
    43621 agcatacggc tggtagagaa gacgccgctg ttagcggaag acgccgcaga ggtcagcacc
    43681 acaaagttga tcacgctggc agccgcaggc aaacctacca acacaaacag ttcaacaaac
    43741 gggctcttct ccgggactac cgaactccac ggcgtcacgg acataatcac aatcagcgcg
    43801 aagacgtaga acataatgat acggatcgga atggagttaa tcgcgcgtgg cagtgatttc
    43861 tccggatctt tggtttccgc agcggttgta cctaccagct caatccctac gaaagcgaaa
    43921 accgctatct ggaatccggc aaagaagcca cttaaacctt tcgggaacca gccgccatca
    43981 ttccacaaat gcgcgaatga cgcttccaca ccagtcggtg actgaaagtg catcgccacc
    44041 ataaccaggc cgacgacaat cagcgacaca atggcgacga ttttgatcat cgcaaaccag
    44101 aactccatct caccgaacat tttcacggtg gcgagattga gcgtcagcag cagcactatc
    44161 accgccagcg aggcgaccca gtcggagaga tcggggaacc agaactgagc ataagcagtg
    44221 atcgccacca cgtctgccat accggttaca acccagcaga accagtaagt ccagccggtg
    44281 aaatatcctg cccacggccc gagtaaatcg gaagcgaagt cactaaaaga tttgtattcc
    44341 agattcgaaa gcagcaattc ccccattgcc cgcatcacga aaaagagcat aaaaccgatg
    44401 atcatataaa cgaaaatgat cgacggcccg gcaaggctaa tcgttttgcc ggaccccata
    44461 aacaaccccg taccaatggc accgccaatg gcaataagct gaatatgtcg gtttgtgaga
    44521 ttgcgccgta gcgactgttc agccggagcc tgatcatcgg caacgacttt tacctgatct
    44581 accatgtttt tttcttcctg tacctgtctg tgttgttcag gctctacggc ctttagtcag
    44641 tctatgatac gacgaatctg tatcgggact catcgatatt aggtaacaat acgcgggatg
    44701 aatactaaga tttagatcta atgttaattt tatgtttaaa gtgagtgtca tatcacggtg
    44761 ataacgcgaa acagctcaca aaaatacacg cttacggggt ctttgcaaca caaaatagcg
    44821 ccgtttggca aactgaaggg tttattgctg aatgcctgct cccctctggt tcgaggggaa
    44881 cagaatgcgc tgcttagagg atttccagca gttcgacttc aaacaccagg gtgctgaacg
    44941 gagggatgga tgcgcctgcg ccgcgctcgc catatgccag ttcctgcggg atagtcagtt
    45001 cccatttaga acctaccggc atcagagtca gtgcttcaat ccagccaggg atcacgccat
    45061 taaccgggaa ttcagcgggt tcaccacgag caacggagct gtcaaacacg gtgccgtcga
    45121 tcagtttacc ggtgtaatga acacgaacgc ggtcggtacg tgccggaatt gcgccttcac
    45181 cctggttgat cacgcggaat tgcaggccag attcggtgct attcacacct tcttttttgg
    45241 cgttttcttc caggtatttc acaccttcag cagccatcgc ctggaaacgc tgacgacgaa
    45301 cggcatcggc gcgttcgtgg atttcacgca gcgcgcgatg caccacatca accggcacag
    45361 ccggatgttt gccttccagc gcatcggcaa tacctgcaac cagtgcttct ggcagcagcc
    45421 cttccaggcc agattcactc agttgttgcc ctacctgcaa accaatgccg tagcttgctt
    45481 gcgcttcgat ggtgtcaaaa gttggggtgg tcatcgtatt tcctttctta gggtataaaa
    45541 gtagcgggca gcatatcagc cacacttctt tgggtaaaac tttgtcgcag gaatgatgac
    45601 aaatcccggg gaaaaagaaa caatagcaat atcccgtatg aatataataa aggcgggttt
    45661 accacggaca tcatagcgtt agcttattca taggctatga ttgaggaaca agacgcggag
    45721 caggaggaaa ccatgcccgg gcgctttgaa ctaaaaccaa ccctggagaa agtctggcac
    45781 gcgccggaca attttcgctt catggacccg ctgccgccga tgcatcgtcg ggggatcatt
    45841 attgccgcca ttgtattggt ggtcggattt ctgcttccat ctgatgatac gcccaacgcg
    45901 ccggtggtga cgcgtgaagc gcagttagat attcagtcgc aatcacaacc gccgacagaa
    45961 gaacagctgc gggcgcaatt agtcacacca caaaatgatc ctgatcaggt ggctccggtc
    46021 gctccagaac ctatacagga aggccagcca gaagaacagc cgcaaactac ccaaacgcaa
    46081 ccattccagc cagatagcgg aattgataat caatggcgct cttaccgtgt cgaacccggt
    46141 aaaacgatgg cgcagctgtt ccgcgaccac ggactgcccg ctaccgatgt ctatgcaatg
    46201 gcgcaggtag aaggcgcggg taagccgctg agtaatttgc aaaatgggca gatggtgaaa
    46261 atccgccaga acgccagcgg cgtggtgacg ggcttaacca ttgatacggg gaataatcaa
    46321 caggtcctgt ttacccgcca gccggatggc agttttattc gtgcgcggta gatgaagggt
    46381 caaccaaaag ccagctaagg attgtctctt tgatgacctt acgtctggct tccagtgcct
    46441 ggggtgaaac catactgatg tgataaacca ggccaagcgt atgctggttg atcaaataat
    46501 atcccccaag cgcagcaata ttaatattca cctgcaatgg gtcgatatct ggcttaaaaa
    46561 tattgtgttt cttcccttca tccaataagg attccattaa ctgcaagtgc gcatggttaa
    46621 tttctaagag acgcagtgat tttgcataat gcacaccttt actttgattt tcactatgca
    46681 caatttttaa aaaccaaggg t
//
LOCUS       DAESHI010000020        46162 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.020, whole genome shotgun sequence.
ACCESSION   DAESHI010000020 DAESHI010000000
VERSION     DAESHI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46162
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.020"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..716)
                     /locus_tag="KW011_001171"
     CDS             complement(<1..716)
                     /locus_tag="KW011_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH7659568.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSSA"
     gene            complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="KW011_001172"
     CDS             complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="KW011_001172"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBH7659569.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAVCAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="KW011_001173"
     CDS             complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="KW011_001173"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HBH7659570.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPEINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3650..4705)
                     /locus_tag="KW011_001174"
     CDS             complement(3650..4705)
                     /locus_tag="KW011_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBH7659571.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4715..5503)
                     /locus_tag="KW011_001175"
     CDS             complement(4715..5503)
                     /locus_tag="KW011_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBH7659572.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5521..6573)
                     /locus_tag="KW011_001176"
     CDS             complement(5521..6573)
                     /locus_tag="KW011_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBH7659573.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6604..7446)
                     /locus_tag="KW011_001177"
     CDS             complement(6604..7446)
                     /locus_tag="KW011_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBH7659574.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSAGNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(7433..8314)
                     /locus_tag="KW011_001178"
     CDS             complement(7433..8314)
                     /locus_tag="KW011_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBH7659575.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8335..9645)
                     /locus_tag="KW011_001179"
     CDS             complement(8335..9645)
                     /locus_tag="KW011_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH7659576.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9659..11190)
                     /locus_tag="KW011_001180"
                     /pseudo
     CDS             complement(9659..11190)
                     /locus_tag="KW011_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
     gene            complement(11402..11716)
                     /gene="pspE"
                     /locus_tag="KW011_001181"
     CDS             complement(11402..11716)
                     /gene="pspE"
                     /locus_tag="KW011_001181"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HBH7659577.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(11791..12012)
                     /gene="pspD"
                     /locus_tag="KW011_001182"
     CDS             complement(11791..12012)
                     /gene="pspD"
                     /locus_tag="KW011_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HBH7659578.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(12021..12380)
                     /gene="pspC"
                     /locus_tag="KW011_001183"
     CDS             complement(12021..12380)
                     /gene="pspC"
                     /locus_tag="KW011_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HBH7659579.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(12380..12604)
                     /gene="pspB"
                     /locus_tag="KW011_001184"
     CDS             complement(12380..12604)
                     /gene="pspB"
                     /locus_tag="KW011_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HBH7659580.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12658..13326)
                     /gene="pspA"
                     /locus_tag="KW011_001185"
     CDS             complement(12658..13326)
                     /gene="pspA"
                     /locus_tag="KW011_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HBH7659581.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13493..14470
                     /gene="pspF"
                     /locus_tag="KW011_001186"
     CDS             13493..14470
                     /gene="pspF"
                     /locus_tag="KW011_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HBH7659582.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14588..15853)
                     /gene="puuE"
                     /locus_tag="KW011_001187"
                     /pseudo
     CDS             complement(14588..15853)
                     /gene="puuE"
                     /locus_tag="KW011_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(15891..17171)
                     /gene="puuB"
                     /locus_tag="KW011_001188"
     CDS             complement(15891..17171)
                     /gene="puuB"
                     /locus_tag="KW011_001188"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HBH7659583.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGRTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(17173..18663)
                     /gene="puuC"
                     /locus_tag="KW011_001189"
     CDS             complement(17173..18663)
                     /gene="puuC"
                     /locus_tag="KW011_001189"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HBH7659584.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDHAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEEAGLPDGVL
                     NVVSGYGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANI
                     VFADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGH
                     PLDTVTTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNA
                     SLSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSV
                     FVNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(18813..19370)
                     /gene="puuR"
                     /locus_tag="KW011_001190"
     CDS             complement(18813..19370)
                     /gene="puuR"
                     /locus_tag="KW011_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HBH7659585.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(19397..20161)
                     /gene="puuD"
                     /locus_tag="KW011_001191"
     CDS             complement(19397..20161)
                     /gene="puuD"
                     /locus_tag="KW011_001191"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HBH7659586.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            20373..21734
                     /gene="puuA"
                     /locus_tag="KW011_001192"
     CDS             20373..21734
                     /gene="puuA"
                     /locus_tag="KW011_001192"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HBH7659587.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            21883..22047
                     /locus_tag="KW011_001193"
     CDS             21883..22047
                     /locus_tag="KW011_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659588.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            22037..23422
                     /gene="puuP"
                     /locus_tag="KW011_001194"
     CDS             22037..23422
                     /gene="puuP"
                     /locus_tag="KW011_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HBH7659589.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLSPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            23556..23801
                     /locus_tag="KW011_001195"
     CDS             23556..23801
                     /locus_tag="KW011_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HBH7659590.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            24114..25757
                     /gene="sapA"
                     /locus_tag="KW011_001196"
     CDS             24114..25757
                     /gene="sapA"
                     /locus_tag="KW011_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HBH7659591.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            25754..26719
                     /gene="sapB"
                     /locus_tag="KW011_001197"
     CDS             25754..26719
                     /gene="sapB"
                     /locus_tag="KW011_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HBH7659592.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26706..27596
                     /gene="sapC"
                     /locus_tag="KW011_001198"
     CDS             26706..27596
                     /gene="sapC"
                     /locus_tag="KW011_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HBH7659593.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            27596..28588
                     /gene="sapD"
                     /locus_tag="KW011_001199"
     CDS             27596..28588
                     /gene="sapD"
                     /locus_tag="KW011_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HBH7659594.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            28590..29396
                     /gene="sapF"
                     /locus_tag="KW011_001200"
     CDS             28590..29396
                     /gene="sapF"
                     /locus_tag="KW011_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HBH7659595.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            29464..29817
                     /locus_tag="KW011_001201"
     CDS             29464..29817
                     /locus_tag="KW011_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HBH7659596.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            30185..30973
                     /gene="fabI"
                     /locus_tag="KW011_001202"
     CDS             30185..30973
                     /gene="fabI"
                     /locus_tag="KW011_001202"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBH7659597.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            31118..32244
                     /locus_tag="KW011_001203"
                     /pseudo
     CDS             31118..32244
                     /locus_tag="KW011_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            32312..34246
                     /gene="rnb"
                     /locus_tag="KW011_001204"
     CDS             32312..34246
                     /gene="rnb"
                     /locus_tag="KW011_001204"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HBH7659598.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(34324..34427)
                     /locus_tag="KW011_001205"
                     /pseudo
     CDS             complement(34324..34427)
                     /locus_tag="KW011_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34481..36471
                     /gene="pdeR"
                     /locus_tag="KW011_001206"
                     /pseudo
     CDS             34481..36471
                     /gene="pdeR"
                     /locus_tag="KW011_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            36618..36791
                     /locus_tag="KW011_001207"
     CDS             36618..36791
                     /locus_tag="KW011_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659599.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            36881..37630
                     /locus_tag="KW011_001208"
     CDS             36881..37630
                     /locus_tag="KW011_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBH7659600.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            37899..38117
                     /gene="osmB"
                     /locus_tag="KW011_001209"
     CDS             37899..38117
                     /gene="osmB"
                     /locus_tag="KW011_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HBH7659601.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(38243..38569)
                     /gene="yciH"
                     /locus_tag="KW011_001210"
     CDS             complement(38243..38569)
                     /gene="yciH"
                     /locus_tag="KW011_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HBH7659602.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(38569..39306)
                     /gene="pyrF"
                     /locus_tag="KW011_001211"
     CDS             complement(38569..39306)
                     /gene="pyrF"
                     /locus_tag="KW011_001211"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBH7659603.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(39499..40668)
                     /gene="lapB"
                     /locus_tag="KW011_001212"
     CDS             complement(39499..40668)
                     /gene="lapB"
                     /locus_tag="KW011_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HBH7659604.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(40675..40983)
                     /gene="lapA"
                     /locus_tag="KW011_001213"
     CDS             complement(40675..40983)
                     /gene="lapA"
                     /locus_tag="KW011_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HBH7659605.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(41132..41896)
                     /gene="pgpB"
                     /locus_tag="KW011_001214"
     CDS             complement(41132..41896)
                     /gene="pgpB"
                     /locus_tag="KW011_001214"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HBH7659606.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            42066..42656
                     /gene="ribA"
                     /locus_tag="KW011_001215"
     CDS             42066..42656
                     /gene="ribA"
                     /locus_tag="KW011_001215"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HBH7659607.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLNTKAEKMGHLLNK"
     gene            complement(42720..45395)
                     /gene="acnA"
                     /locus_tag="KW011_001216"
     CDS             complement(42720..45395)
                     /gene="acnA"
                     /locus_tag="KW011_001216"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HBH7659608.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKV
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(45559..45654)
                     /locus_tag="KW011_001217"
     CDS             complement(45559..45654)
                     /locus_tag="KW011_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659609.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(45768..45935)
                     /locus_tag="KW011_001218"
     CDS             complement(45768..45935)
                     /locus_tag="KW011_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659610.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(45938..46102)
                     /locus_tag="KW011_001219"
     CDS             complement(45938..46102)
                     /locus_tag="KW011_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HBH7659611.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
BASE COUNT        10923 a        12204 c        11623 g        11412 t
ORIGIN      
        1 gccgatgatc caataaatcc ggcaacaaac atattcgctg gttggttata aaccgttttc
       61 ggcgcaccaa cttgctgaac aatcccatcc ttcataatca caatccgcgt cgccatggtc
      121 atcgcttcgg tctgatcgtg ggtcacgtag atcatggtgg tattcagctt ctggtgcagc
      181 ttgctgatct ctgcgcgcat ttgcacgcgc agcttggcat caaggttaga gagcggttca
      241 tccattaaaa acacgcccgc ttcgcgcaca atcgcccgcc cgagcgccac acgctggcgt
      301 tgcccgccgg aaagcgcgcc cggcttgcgt ttcaggtact cacgcaggcc gagaatttgc
      361 gccgcccagt tcacccgctc atcaatcacc tctttggcga ttttttgcat cttcaggcca
      421 aacgccatgt tgtcgtaaac cgtcatatgc ggatacaacg cgtagttctg gaacaccatc
      481 gctatattgc gtgctttggc tggaacgtca ttcattcgtt tgccgtcgat caacagatcg
      541 ccgccgctga tctcctcaag cccggcaatc atgcgcaggg tggtcgactt accgcagcct
      601 gacggaccga caaacacgat gaactcttta tcggcaattt ccaggttgaa gtccttcacc
      661 acatgcacct ggttatcgta gattttttga atatgttgta acgaaagctg agccatgttg
      721 attcctttgc tatacgtttt gccagaaggc cgataaccgc gaccaggtca gggagtccgt
      781 tgaaggcaat agtaattgcg ccccggttat gcccgcgccg atccccaccg agcgcatacc
      841 gctggcgtta atggcgtcaa tgcccgcctg cgcatcttca atgccgatac atgcctgcgg
      901 cggcacgccc agcccggcac agacagcgag aaagatttcc ggatccggtt tcgagttttt
      961 aagttgggaa gcatccgcgc agaaggtgaa aaactcacgc agctccagtg catctaaaat
     1021 cgtcggcgca ttgagggaga cagaagccag ccctaccgag atctgttgtg cgcggagatc
     1081 tgccagcaaa gagcgaatgc cgggtagaac agcgttgacc gtcaactcgc gcaatgagtg
     1141 gacatagagc agatttttgc gatacgccag ttgcgccctc tcctgcgagt taaagtcgcc
     1201 ctctttgccc ccgtgttgca gaatgcgccg cagagactca tcgcggctga tcccttttag
     1261 ggattcgtta aactgcgcat caatgctgat gccaatttca gcggcaatct gctgccacgc
     1321 ctggaaatgc agatgcgcgg tatcggtgat tacaccatcc agatcgaaaa ttaccccttg
     1381 cagtttcatt catcctcctg atgtgtggca gcggtcccat tgaagggcaa aataaaatca
     1441 cccaaacaga aaacagattc ttcagccacg gatataagct gaccgttcaa acgcagtgaa
     1501 acgggggcag aagttcgaat cgcaatacgc tgcgcgtcaa gagtgacctg taattcgcag
     1561 ccctgccaga acagagggaa agacaactgt tgccactgct caggtaacgc cggattgaga
     1621 tgcaattcac cgtcacgcac gctcaccccg gcaaaaccct gaatcgcccc cagccagata
     1681 gcgccagttg cggcagcatg gataccatca tcacaactat gcggatcagc accaagatcg
     1741 atttcagtcc cctcgcgcca gaactgataa ctttgggtca gcaggccaca gcgtgcggca
     1801 acaatgccgt ggattgcttt acttaatgac gagtcgtgaa tagtgcgcgg ttcataaaat
     1861 tgcagattgg caagacacga tgccgctgag aactgctccg gcagcatgta attgagcatc
     1921 accacatcag cttgtttgag gatttgcatc tcgttcactt ctgcgcgtga ataatccagc
     1981 agaatggttt gcttccccgc cgccgctttg tatttcgcaa gattaatctc cggcttagcc
     2041 ataaacgaat catcctgcgg caaaacgccg tcgggctgaa tttctggcag ccgcagttct
     2101 ttaaggaaca tttcggcacg atgaataaac gcgtcgtcgc tacagccaaa ctggcgggca
     2161 atactcagcg cctgctggac gttgtaatac gccatatagc tggtgaaggc gttattattg
     2221 acatgttcgg tatattcgtc aggcccaata acatcatgaa tttccagacg gtcgttaacc
     2281 ctcaccgcgc ggctaatcca gaactttgca gtttccagaa gtagcgccat gccttcatgc
     2341 gcaatgaaac tttcatcccc cgtggtctgc cagtattgaa taaccgccca ggcgatatcg
     2401 gccaccagat gatgttccgc ctgcgccgag gccacttttt gccgcagccc ggtgcgaatg
     2461 ttaatggcgg caaattccgg cgtctcttct tcgccgctgc gcgcgctttc ccacggaaat
     2521 agcgcgccct gccagccgtt gcgtcgcgct ttctcctgcg cgcctggcaa gttgtgccag
     2581 cgataacgca gtaaacttcg ggcaaccgtc ggatcgctaa acagatgaaa cggtaacaaa
     2641 aatacttctg tatcccagaa aacgtggcct ttgtagcctt cgccggttaa gccttttgcc
     2701 gcaatgctgc tgcgctcgtc gtgggcaggc gtcatgatgc gcagatgata aagcgcataa
     2761 tctaacgctt gctgatcgtg cgcttcgccg ccatttaccg tgatacgacg tttctgccac
     2821 cattgacgcc agttttctgt tgatactgca agaagttggt cgtaactctg ctgcgcgcac
     2881 atttcaagct ggcgaagcga cgcgctgccc cactcgtcta aaacggcttg cctgtcatcc
     2941 cgccagtcga tccacaccag tttttgcaac gtcactgtct cgcctgcatg aagctgcgca
     3001 ctggtatgtt gcagtaaacg gcgctctttg gcggtatagc attgctgcac atcaccgctc
     3061 accttacagc aacagctgat ggccacatca ctgcgtccat cctgggtggt gtagatcccc
     3121 tgcatcaaat gctgaccaaa cacccgcacc tgggtttcgt cgagatgctg gcgaccgtgg
     3181 ttggtctgcg tggcgtcaat gcctgttgaa atcagcactg acgcgtcggc gtccagtggc
     3241 gtaatagtga tttccagcac aatgagcggc agttggtctg ccgaaacaaa gcgacggctg
     3301 gcgatggtgt aacctgtgcc gttgctggta cgccagacaa cgctacggcg taattctcca
     3361 ctggcaaagt caagttcacg ctgccaggct tcgcgggata acgagaaaac ctcaccattt
     3421 atggcaatct ccatccccac gacatcaggc aggttcacca gttcgttgat ttcacctttt
     3481 cccgcccgat gatacagccc cgccagatac atccctcgcg tctggcgcgt gtaatcttct
     3541 tcatggctgg cgcgaagccc aagatagccg ttcccctgcg ccatcagcga tgcatacttg
     3601 ttcagggtat gctggctgaa atggggttct gataacgtta ctggcctggt cataattcca
     3661 cacgcgtccc tgtttctgcg gattgataca acgccgccac cagttgctgg atgatgtacc
     3721 cctgcttggc gtcggctatc atcacgggct tgccctgtac gtgattgata aaggcttcca
     3781 tgctgcgcaa atggcggttg tcgtctgcca ttttccgttg catcagcgtc attaattcac
     3841 cgttgttatc ggtgtagata tgtgctggaa acagcgtcgc accagcttta tcaccacaaa
     3901 agctgacgtt cataatcgac tgttcgcgga tgttgagtgc aaatgacgtt tccagccaca
     3961 gaatgccgcc gttatgaaat tcaatggtgc caaacagcga atcttcgacg ctgtaagttg
     4021 ccggatccca ctcaccaaat tgaccacagc tcttttgcgt gccgatcttt tgaaagctat
     4081 gcgcattcac gcttttcacc gccggaaagc ccagcacata catcgcagca tccagcatat
     4141 gaatgccgat gtcgatcagc gggccaccac cctgcagttc tttattggta aagacacccc
     4201 agccgggaac gccgcagcga cgcagggcgc gggcggtggt aacgtaaatc tctcccaaaa
     4261 cgccgttggt cacctgttca cgcagctgtt gcgtatcgag cgcaaaacga tggtgaaagt
     4321 cgtaggccag caccttgccc agtttgcgcg cggtatcgca catttcccgc gcctgttctg
     4381 gcgtcatggc gagcggtttt tcgcacatca catggcagcc cgcttccagt gccatcaggg
     4441 tatgttcgta atgaaaacgg ttaggtgagc agacgctaac cacatcaggt ttcaccgcca
     4501 gcagcatggc ctgcggatcg tcccacacgg atgcattccc gtatttttct gccagcgcct
     4561 gcgcctggga aaggcggctg tcacagacag ccaccagttc cagatcgttg cgggtgcagt
     4621 agtacgaagc atgaacctta tccgccacct ggcctgcgcc tattatcgcg acccgcagcg
     4681 gagagcttgt cattgcactt ttcacaaaga cctcttagca ggtacgcaac caggccaacg
     4741 aatcacggta cgcctgggca ggatcttccg cccggatacg cccttcgtac actacatagc
     4801 cctgatagtt atccgcgcgc agctgttcaa acagcgcgtg gaaatccagg gtgccgctgc
     4861 ccggctggta gcgatggtta tccgcaatat gcacatgacc gagcaggtcg cggttgtcat
     4921 gcagcgcctg cgccaggtta tcttcttcga tattcatgtg atagaaatcg ccgataatct
     4981 gtacatgttt aagatcgttt tcgacgatgt aacggcgggc atcggcgagg gtgttgatca
     5041 tatgatcctg atagcggttt aacggttcga gatacaccac tgttccggta cgcgcggcga
     5101 cctgttccag tacgcgcagg gaatcactca ccattttgcg gtcgccgtcc aggctacgcg
     5161 gcgaggtcat cggcggtaag cggaaggtaa acatgcccca cgctgccgga acgacgatac
     5221 ctttatcccc cacttctgcc agcgcttcga gaatgcgttc gatctgcttt aagccattaa
     5281 gacgacgctc ttcgataaag tcgccaatcc aaccgtcata gccaccgcag gcggtggtca
     5341 ccggtaaacc ggtttctttg attgctgctt tgacttcttc gagattgttg accagcagtt
     5401 tgccgtcaat ctcaaaacca tcgaagccca tctctttgat ataacgaaat ttctccagaa
     5461 tgttttccgg aaagaatgcc tgattctgtg tgcctatttt cattttttca ttcctttgaa
     5521 ttaaaacgta acgcccattt tgatgctctg ttccggatgc tgatcgacat actgcatgta
     5581 gctttccggg ctattggcaa aggtcactac cgggtcgatt aaatcttcgc aattgagata
     5641 accgttcatc agcagttccc agcaggtttc ttcaatacgc ttacggctcc agcgcggata
     5701 atccgggttc ggttcgctgc acgcgcggga gaaaacaatt ttggcgttat tgaaatgcgc
     5761 ttcgcgtccg aggttaaaac cttcagcaaa tggtttggca aacgcaacat aggagatggt
     5821 gccaccgtag gccagaccgc ggaacgccga ttgcagcgcg tcggcatagc cgctggtttc
     5881 gataatcaca tcggcaccct gcttgccggt cagcgttttg atctctttgc ctacgtcagt
     5941 accaatgggg ttaaggcaga aatccgcgcc gtggcgacgg gcaatatcac agcgatgagc
     6001 gataggatcg acgccaatca cgaccgacgc gccagcacgt ttagccagtt gaatggcaat
     6061 ttgaccaatc gcgccaagcc ctaccaccac aacaaaatca cccacgcgca cgttggcatc
     6121 gcgcacgccg ctcatggcaa actgcgccgg gtcgtaacaa acggcgtttt tccacgagct
     6181 gccttccggc attttgcgca gcttgtagtt attcaccgcg ttaatgatga ccgtctcgga
     6241 gagcgggccg tagccgcata cgctgtcgcc caccgcgtag tcggtaacgt cgctgccgca
     6301 ctcgataatg tcgccaacca ccatgtttcc aagctggaat ttgccaaact caatgccgcg
     6361 cggcgcatct gccggacgcg gagtgaacat ctgccattcg ccgttaaagt cttcatcgat
     6421 aaacgggctg gcggcgcgga agtcgaccac ttccgttccg tgtttcggtg cgccgaaacg
     6481 cacgcggacc ttcacttcat tagctaaaat cgcccgatct tcatactcaa ccagcgcagc
     6541 aacacgcggt gctgtggcta ctaacttttt catttaaaac tccttgtaaa gaaactggcc
     6601 gctttagccc ttcacgccac cggcggtcaa accactctta ataaaacgtt ccgaaagcgc
     6661 atacataatc accaccggca atgcggtcac cagtgaggcc gccatcatcc gtccccagat
     6721 gtaatctggc gtactgaaca gcgcgttcag gcccaccggt aaggtgaaat tgccggcgct
     6781 ggagaggaaa atcgacgcaa acagatagtc gttccacgcc accataaagc agtagacgaa
     6841 cacggatatc aacccggaca tcgccaacgg cacggtgatg cggaagataa tttgcagacg
     6901 gttgaggcca tccatcatcg ccgcttcttc gatctcatcg gggatggtgt cgaagtagct
     6961 tttcagcata aacacggcgg tgggcagagt ttgcgtcacc atggtgatga tcagcgccat
     7021 ctcagtgtcg taaatgccca gcgcggtgat gattttgaac agcggcacca ccagcaaaat
     7081 gccagagaac atatacaccg tgtaaaagct ggcgttgatg gtcatccgac ctttaaagcg
     7141 caggcgggaa agcgcgtaag ccccaagaat gccgaggaat accgccacca cggatgaaac
     7201 cacagacacc accagactgt tacggaagta gtcgacaaac gggaaaatca tcgggttaaa
     7261 aatgtcgacg taatgctcca gcgtccactg ctgcggcagc agcgtaggat gcagtgagat
     7321 cgcctctttc gcgcccttga acgaggtcat cagcatcaca aaaaatggaa acagcgtgat
     7381 gatgagaaac agcgccagcc cgcagtaaaa accgatgcga ctgagtgtgc gtttatttgt
     7441 tgccattgag gttcacccgt ttcctggtca gcagaatgac agcgaaaata atgatgaaga
     7501 gcaccaccga aatcgccgcc gctttcccta agtcattaaa ggcaaaggcg gttttgtaga
     7561 gatagacgcc gagaatatcg accttggtgg tcagcagata aacatcagca aacatgtaga
     7621 acatccagat agtgcgcagg gtcaccaccg tcgccaggac cggcataatt gcgggcagcg
     7681 tgacgatgcg aaaccgctgc caggcattag cgccatccat ctctgccgct tcgtacagcg
     7741 atttgtcgat ggtctgcaaa atcgccagaa acgagataaa ggcatacggg aagtagcgcc
     7801 agatggcgaa cagcaccacc agcacaaagc tactgcccgg attatcgaac cacagcggtg
     7861 cctgctcata gagatgcaga agatcgacgc cgaggtagtt cacaatgccg tagccgttgt
     7921 tgaacatgta tttccaggca aacaccagcg aaatggacgg cgttacgtag gagaggatca
     7981 ccagcgaacg cgcggttttg cgcaggcgga actcgcggtt gaaaaacatc gccactgcca
     8041 gcccgagcac ggtgctgccc gccaccacca gcgcggtata ccagaccgtc atccacagcg
     8101 agtgccagaa gccgggatcg gagaggatac gcacatagtt actcacccca acaaaggttg
     8161 actcgatgtt gggattgagc ggcagacgta aaaaactgat ttcgatattc gacaccattg
     8221 gccaggccac cagaccaccc agcagtaata agctgggcgc gagaagcagc agcgcaaagg
     8281 gcatatcgga acgacctgaa aaaagcctgt tcattgcgga ttcctgtcct gggtttagtg
     8341 ctgttcgatc agttcatcca gctttttctg gctcgcttgc agcgtgccag ggagatcggc
     8401 tttacccacg gtgacgttat gcaccatgga gctcaccacg ccagaacccg tcacatcacc
     8461 catgcgggta aagtttttat cccctactgc gccaaagacc tgaatatttg gcagttcacc
     8521 gattagctga ttcggtagtt cacccagcgc cttaataacg tcgttgtctt tccaggtggc
     8581 ggtagtgaca accgctttat tcaccggcag cgcagcacct ggcgacatca tcacccagtc
     8641 ggcaatgttg tctgcctgct ccataaaggt gacaaatttt tctgctgctt ccgtctcttc
     8701 ggccttttgc ccggcggtaa tggtcagcga ggtcaacatg ccgtagaccg cagagttttt
     8761 ctcggttggc accacgaaac cgacgttttt cgggtcgcct tctttaatta cagccggaag
     8821 gatataggtg gagtaaatcg ccatcggcgc ggtgccgttc ataaaggcgt ctttcacttc
     8881 catgatgtcg ttagaacccg gcatgtcgtt agaacccggc atagtgttgg cagcaaggtc
     8941 gcggtaatag gtcagtgcct gcatcatctc tggtgtatca agggtgattt tgccttcggc
     9001 gttaaagacg ttagcctggt tggataacgc aaactgggag aaggattgtt ccgtcaacac
     9061 gctttctgct gtaggcagcg caatgccgta ttttttattc gccgggtcat tcagtttctg
     9121 tgcaacgtcc agtagctgtt gccagttttt cggctcctcc agccccgctt ttgccagcac
     9181 atctttgcga taccagatgc cgccaatcca ggcgctgacg ggaacaccgg tccaggcact
     9241 cccatcttcg gtgcgcacaa tacgcagtac gccatcgtaa aacgcgcctt caccaacgtt
     9301 gctgatgacc gtggcaaccg tcttgcgatc gataagctgc tctttatcca taactttggc
     9361 gtagtcatgg ctggtttcga tcacttccgg cagcgagccg ctacgtgaaa gggtgatgac
     9421 tttggtgtta taagcgtcct cttccacggg cacttgttta acggtgatac ctgggttttc
     9481 tttttcaaaa cgggcaatca atttgctgat aactgcctga cgctcctgct ccaccgaaga
     9541 atgcataaat tcgatggata catgggtttt attttcttct ttacagcctg aaatcagggc
     9601 gcaggaaacc agtgctgata acagcacgat ttttgattta atcatgagat gttccttttt
     9661 atttaatcca cataacctgc caggggcgca gcgttatttc attacccaat agatcaactt
     9721 cactaatcag atcccgacca tgcagattag taatatttac atgctgaata tttttactga
     9781 cattaaacaa tccagtcaga caattaccat cagcgttact tctttgaata cgcattacgg
     9841 atgaattaat cgtatcgatg gtaaaattat tatccggatg aaactcgtta tggctgcgac
     9901 gaagtgtaat taaacgcgac aactcatgat ataccgcatg ccttaatgta gcttcatcgt
     9961 tcagttctcg ggttatctct ttactgtgat attttttacg gttaatcgca cggttatatc
    10021 cgagtttttc gacacctgcg taatcattac gcgagccaag aatactttga atatatatcg
    10081 ctggcacacc ggggaaactt aacaaaatcg catgggcgag aataaacctg gcgcaacgtt
    10141 cttcatcgct actctcacgg cggcttaacg catccatatg tgagaggcga ggaagttaaa
    10201 ccaggtggtg ttgctggaag gtagtgtcag gttttgcgcc cacgcacaaa gcgcctcaac
    10261 gttctgtttt tgcaccgcat gcagcaccag tggcggcagc gagaactggt acaccatatg
    10321 tgcttcgtca tcgcctgcgc caaagtaagc aatgttgtct ttatgcggaa cattggtctc
    10381 ggtaatgatc actgtacctg gcgcaacgtt atcaataatc gaccgtaaca gtttgataat
    10441 cagatgtgtt ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac
    10501 ggcatccaag cggacatact cagcaccttt cgcaaggtaa cagagtaaaa catccaccat
    10561 cgccagcaac acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt
    10621 ccataaatgg cgcgttgaat gatcgcgcat ctggaatggc gttaataacg gtaacgcacg
    10681 cgggcgagtg acggcgctga gatcggtttg cggatcaacc gcaataaaaa aatcttcaaa
    10741 acctggctgc tgttgtaaat agtttttaaa ccattcactt tttgccgaca tatggttgca
    10801 gacaaaatca aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc
    10861 cgcttcactg gcgacctgat gataatcaat taccgaaaac ccatcatcag atgaacatgg
    10921 ataaaaaggt aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtaaaactg
    10981 attaaatgtg ggtaatggtt ttaaatcatt gctgtgaaat tgatcggcat aggtaattaa
    11041 cacgacatca ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact
    11101 ctcaagacgc gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc
    11161 gtccaggtaa tccgtaattt tctgtttcat gttgcctttt tgtggtagcg gtcccatgaa
    11221 acgagagact actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca
    11281 ggaaatcatt ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatggg
    11341 gatgtgaata aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct
    11401 tttaaccttt gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg
    11461 tatatcccat ctcgctaagg atctcttttg cttgccctga ctggcgtccg gcattgcaat
    11521 acactttcac ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct
    11581 tcaggggaat attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa
    11641 catcgatcca gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta
    11701 agcctttttt aaacatgtaa gcgtccttct atggatgaaa attgccatcc attatacgcc
    11761 tgagatttaa caatttttgc gaaactcccc tcacctttta taacgctgtg ccagtttatt
    11821 agcagcccga cttaacagcg gttccagcgc cacagccagc aacattttca gcggtcggcg
    11881 agcaactgat tttatcgccc agcccgctac acccgccggg ccatagcgca gtgcggtaag
    11941 aagtaccagc ttgcctgcta atttgaaacc aggctttacc ttttgcccgg cctgttgcca
    12001 gcgagtattc ataactttcc tcacagttga cggaaacggc tacgtaacgt gaaagtatcg
    12061 gaagtgacat aacgttccat ctcgcgtaaa cgcgtttcac ttgccgccag ttcacggtcg
    12121 acttcatcca gcaattcgct gctggaaggt agctgctcac caaaggccat gttgtccggc
    12181 attggatcaa gcgcaaatga caaaatgatg taagcaacca gggtaaacag cgccagaccg
    12241 aagaaaatcg acagcaccac caggatacgc accagtttta ccggtacatc aaaatagttg
    12301 gcaatcccgg cgcagacgcc gcggaccatg ccctgctgtg gaatacgcca taatttttta
    12361 ttgagattaa tgcccgccat tagcgatccc tccagttcgg atgttctgca tcaagaatag
    12421 attccagcgc ctgaatacgt tcgcgcatcc gttttgcttc atcagccagt tgcgctaatc
    12481 gctgctgctc actttgcgac aattcatcgc gaccagaacg attgctgtaa tgcagccata
    12541 accagatcgg taaaacaaac agcacaaaaa tggttaacgg aatagccaga aatagcgcgc
    12601 tcataagtac tccttagacg atgagcggcg gcgcgttcgg acgccgcctg gatgttatta
    12661 ttgattgtct tgcttcattt tggcttttaa ttgtgccagt tgttcgctga ttgcatcatc
    12721 ggctttcagt tcggcaaact gatcgtccag cgatttttgt ttaccgaagc tgtggctttc
    12781 tgcttccgct tccatctggt caattcgacg ttcgaaagat tcgaaacgag ccattgcttc
    12841 atccagtttg ccactgtcca gctgacgacg cacatcgcgc gacgagtttg ccgcctgatg
    12901 acgtaacatc aatgcctgct ggcgagcgcg tgtttcgctc aatttgtttt ccagctcgcc
    12961 aatctctttc ttcatgcgtg ccagcgtatc gtccaccagc gtcacttcat gttccaggga
    13021 cttaatcaga tcggtcagtt tctgtttttc aattaacgct gcacgtgcca gatcctctct
    13081 ctctttcagc agcgccagtt cggctttttc ctgccattca acttcacgcg ccgacgcttg
    13141 ttcaatacgg cgagtcagct gtttcttttc tgccaacgca cgcgccgaag tagaacgtac
    13201 ttcaaccagt gtatcttcca tctcctggat catcagacgc accagtttct gtggatcttc
    13261 cgctttctct aacagagcgt tgatgttggc attcacgatg tcggcaaagc gagaaaaaat
    13321 acccataatg ttgtcctctt gatttctgcg ttcaggattg tcctgctgaa ctgttaatac
    13381 aatttgcgtg ccaatttttt atctttttga tttataaaga tctgattgaa gaatcaacag
    13441 caacatgcca ggatgaatta gcgaattaca ctaacaagtg gcgaatttca tcatggccga
    13501 atacaaagat aatttacttg gtgaggcgaa cagctttctc gaagtgctgg aacaggtttc
    13561 gcatctcgca ccgctggaca aaccggtgct catcatcggc gaacgcggca ccggtaaaga
    13621 gctgattgcc agccgcctgc attatctctc ctcccgttgg caagggccgt ttatttccct
    13681 taactgcgcg gcgttaaatg aaaatctgct ggattccgaa ctgtttggtc acgaagcggg
    13741 ggcgtttacc ggtgcgcaaa aacgtcatcc agggagattt gaacgtgccg acggcggtac
    13801 gctatttctt gatgaactcg ctacggcacc gatgatggtg caggagaaat tattgcgcgt
    13861 gattgagtac ggtgaactgg agcgcgttgg cggcagccag ccattgcagg tgaatgtgcg
    13921 gttggtatgc gcgacgaatg ccgatctccc ggcgatggtc aatgaaggca cttttcgcgc
    13981 tgacctgctc gaccgactgg cttttgatgt tgtacaactg ccaccactgc gcgagcgcga
    14041 aagcgacata atgttgatgg cagaacactt tgccatccag atgtgtcggg aaatcaagct
    14101 gcctctgttc ccggggttta cggagcgcgc cagagaaaca ttgctgaatt atcgttggcc
    14161 gggaaatatt cgtgaattga aaaacgcggt ggaacgttca gtgtatcgcc acggcaccag
    14221 cgattatccg cttgatgaca tcattattga tccctttaaa cggcgtccgc ctgaagacgc
    14281 tatcgccgtt tcagaaacca cctcgcttcc gacgctgccg ctggatttac gtgagtttca
    14341 aatgcagcag gaaaaagagt tgctgcaact cagtttgcaa aaggggaaat ataaccagaa
    14401 gcgcgcagct gaattgctgg ggttaaccta ccatcagttc cgcgcgttgt tgaaaaaaca
    14461 ccagatttag cgaagttggg caatgcgatt cgccggatgc ggcgtgaacg ctttatccgg
    14521 catacataat caacgtcgtt tgcaggcctg ataagcgcag cgcatcagac attattgcgt
    14581 tgggctatta atcgctcagc gcatcctgca aaattttcat tgccgcatcg aattgcgcat
    14641 ccgggatggt cagcggatac aggaagcgaa tcacgttgcc gtatgcgcca caggtcagca
    14701 ggagcagccc ctgcgccagc gcgcgttgct ggattttctg tgcaatcgcc gctgacggct
    14761 cgcccgtttg cggatcgtta aactctgccg caatcatcga ccccaggccg cgtaccgcag
    14821 caatggccgg aacgctttct ttggcatcaa tcaacgtgtt tttgagacgc tggcccagtt
    14881 gattcgcgcg ttcgcagagt gattctttgt tgataatatt gagcaccgcg tgcgcggcag
    14941 ccaccgccag cgggttcccg gcgtaggtgc cacccaaccc gcccggcgcg ggcgcgtcca
    15001 taatattcgc gttaccgacc acgcccgaaa gcggcatccc gcccgcgagg cttttcgcca
    15061 tcgtcattaa gtccggctta tcggcgtaat gatccatggc aaacagttta ccggtacgcg
    15121 caaagccgct ttgcacttca tcggcaatca tcacaatacc gtgctcgtcg cacaggcggc
    15181 gaatggcggc aaccagctct tttggcgcaa cgttgaaacc gccctcgccc tgcaccggtt
    15241 cgaaaataat ggccgcgacc tgcttcgctt cgatgtctga tttaaacaag cgttcgatgg
    15301 cgtcgaggga gtcctatgtt gaaatgccgt gtaaatctga cggataaggt acgtgataca
    15361 ccgaaccagg gaacgggccg aagccgattt tgtacggcgc aacttttccg gtcagcgcca
    15421 tggtcatata tgtacgaccg tggaagccac cgctaaacgc aatcacgcca gggcgtccgg
    15481 tatgggcgcg agcaattttc accgcgtttt ccaccgcttc cgcaccggta gtgaagaacg
    15541 cggttttggc ctgcccgctc accggggcaa gggcgttgat tttctccgcc agggtgacgt
    15601 agctttcata cggcacaatc tgatacgcgg tgtgggtaaa ctgttgcagt tgctgctcca
    15661 ccgccgcgac cagatcaggg tggcgatgtc cggtattcag caccgcaatg cctgcggcga
    15721 aatcgatgta ctcgttgccc tcaacatcct tcagagtagc gttttcagcc gactgggcga
    15781 agaagttaca catcacgcca accccgcgtg gggtagcaga aagacgacgc tgatggaatt
    15841 cattgttgct catgtttcac accttttgac tcttcactgt taatcggaaa ttaaacgccc
    15901 agacgatcgc gcaggctgta ccaggctgcg cccatcgcgg taaacggtac tcgcagcgtg
    15961 cgcccgccgg ggaacgggta atacggcaga ttggcgaagg catcgaaacg ttcggcgtcg
    16021 ccgcgcagca gttcggcaat caaacgtccg gcgagatgag tacaggtgac gccgtggccg
    16081 ctgtaaccct gcatgtaata gatgttggta tcaaggcgac caaactgcgg catgcgcgac
    16141 agggtcagca ggaagttgcc cgtccagcgg tagtcgattt tgacgccctt cagttgcggg
    16201 aaggttttca gcagtttcgg cacaacaaga cgctcaacgt catccgggtc gcgcgcgccg
    16261 tagaccacgc cgccgccgta cagcaaacgg ttgtcggcgg taagacggta gtaatcaagc
    16321 agataattac agtcttccac acagtagttt ttcgggatca gcgaacgggc taaatcttcc
    16381 gacagccgtt cagtggtgat cacctgcgtg ccgcacggca tgctgcgttt cgccagttcc
    16441 ggctctactt tatcgcccag atacgcattc ccggcgacaa tcacatactt cgccgtcacc
    16501 tgacctttgg cagtgcgcac caccgctggc gtggtgtgct ggatctgcgt cacggcagaa
    16561 agctcataca cgcgcccgcc gttcaggcgg atggcatcgg cttcaccaat ggcgaggttt
    16621 agcgggtgaa tatgcccacc gctgtgatcc aacagtgcgc cagtgtagcg atcgctggcg
    16681 acctcgcggc gaatggcgtt agcgtccagc agctccagct gtttattgcc gtagcgttcc
    16741 cagttctctt tctgctcttc gagtgtggcg agctgtttat cattcatcgc cacaaacagg
    16801 ccgccggggc ggtagtcgca gtcaatctga tagcgtttga tacgttcgcg gatgatctcg
    16861 ccaccctcga acatcatgct gccgagcata cgggcggtgt ccatgccgta gcttttttcg
    16921 atcacgtcga tgtcgcggct gtaggagttc acaagctgcc cgccattgcg cccgcttgcg
    16981 ccaaagccga tgcgtgaggc ttcgagaacc actacgtcaa agcccgcttc cgccagatgc
    17041 agcgcggagg agagcccggt atagccgccg ccaaccacgc aaacgtcgca ggtgattgac
    17101 tcattcagtg tgtcgaatgg tgcatattta ttcgcactgg cggcgtagta actgctggta
    17161 tgttcggtca tttcaggcct ccaggcttat ccagatggtt ttcagttcag tgaatttttc
    17221 aagggcatgc agggatttgt cgcgaccgtt gccgctctgc ttatagccgc caaacggcac
    17281 ggtcatatcg ccgtcgttgt agttattgac gaagacggaa ccggctttca ggcggcggct
    17341 catacggtgc gcgcgggaga ggtcgcgcgt ccataccgcc gcgccaaggc cgtactggct
    17401 gtcgttggca agctgtagcg cctggtcttc tgatgtgaaa cgcgtgacca ccagcaccgg
    17461 accgaaaatc tcttcgcggc ttaaggacgc attcgggtca acatccacaa agatggtcgg
    17521 gccgatggcg gcagccagct cagcgttacg accatccagc agcagttgcc ctttgctttc
    17581 gccttcacga ataaagctat ggaccgagtc ggcgtgggcg caatcgatta aggtgcccat
    17641 ggtggttacg gtatcaagtg gatggcccgg ctgccagttt tgcgcctgct gttttaacag
    17701 ggctaagaat tcatcggcga tgctctcttc cagtaacaaa cgcgttccgg cgatgcacac
    17761 ctggccctgg ttgtagaaga tgcctgcggc ggtggcgctt gccgcttttt gcagatccgg
    17821 acaatcggcg aaaacgatgt tggcgctttt gccgcccgct tccagccaga cgcgtttcat
    17881 gttgctatca cccgcgtctt tcagcagttg tttacccgta cgcgtggagc cggtaaaggc
    17941 gatggcatcg atatcgttat gacgcgaaag cgcctgcccg gcttcatgac cataaccgct
    18001 cactacgttc agcacgccat ccggcagccc cgcttcttct tttgccagcc cggcgagacg
    18061 aatcgcactg agtggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag
    18121 cgccgggccg agtttccagc aagtcagcag cagcgggaag ttccacggca cgatggcggc
    18181 aatcacgccg accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac
    18241 ttcgccatac actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg
    18301 aatatcatca cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc
    18361 cagctcttcg gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt
    18421 agccggagaa gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc
    18481 atggtcgata tcgacgctct tgccgcgggc aattttcgcc agcggtgcct gggtgaccgg
    18541 atcaacggtt tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa
    18601 gcggttttca atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt
    18661 cattatgact cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag
    18721 agatccccgg gccgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc
    18781 attgcgctta cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg
    18841 ataattcggc aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg
    18901 gcataacttt gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca
    18961 ccttccagta ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg
    19021 cccggctggt acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc
    19081 agcttcattg acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc
    19141 tgcggctcat caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc
    19201 ttcagcagct tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg
    19261 atagcactgt gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc
    19321 tggcggattt ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt
    19381 ccttaaactg tagtggtcag agtcgctgtt tttcagcgat atggtgctga caagcggtga
    19441 taaagccctc gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc
    19501 actgtacgcc cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg
    19561 gcgagcgagc ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag
    19621 agtttaccca aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa
    19681 cctgaacttc atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat
    19741 gttccagcaa ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca
    19801 ccagttcttg taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat
    19861 taattatcgc catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt
    19921 caccatatag gtgcggctgc acattgctgg gactaccagg aagataaatg ccatcgagtt
    19981 tcggcaaaag ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca
    20041 agccgcctgc gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac
    20101 ccttaagcct gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca
    20161 tgctcaatct cacaaagtgg actaaattat cgccattact gcgctttaaa tgacctttat
    20221 gttcaatatt ttttcaatct agcagtgggt tttcattttt gcaaactcaa tttaacattt
    20281 gacaaacatt tagtttgcat acagattcga atggtggtca ttatatttta cgctttgata
    20341 acgagcggaa aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga
    20401 actttgttca gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct
    20461 acctggagcg ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt
    20521 gcttccgtgg aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt
    20581 tcccggcctc ggtatttgca atggatattc tgggcaacgt agtagaagaa gcaggtctgg
    20641 gtcaggaaat gggcgagccg gatcgtacct gtgttcctgt tctcggttcc ttaactcctt
    20701 ctgccgccga tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg
    20761 ctccctttga cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc
    20821 gcgacgctga agggaatctg caaccgccct gcgcgcctgg caccgatgac cgcaatacac
    20881 aaagccaggt ttactccgtt gataacctca accacttcgc cgacgtgctc aatgatattg
    20941 atgaactggc gcagttgcag ctgattccgg cagatggcgc ggtcgctgag gcctcgccgg
    21001 gtcagtttga aatcaacctt taccatactg ataacgtgct ggaagcctgc gatgatgcgc
    21061 tggcactaaa acgcctggtg cgtctgatgg caggaaagca taagatgcac gccactttta
    21121 tggcgaagcc gtatgaagag cacgcgggca gcgggatgca tatccatatc agtatgcaaa
    21181 ataatcgtgg cgagaatgtg ctttctgaca cagaaggcga agattcgccg ctgctgaaaa
    21241 agatgctcgc cgggatgatt gacctgatgc cgtcgtcgat ggcgttgctg gcaccaaacg
    21301 tgaactcgta tcgccgcttc cagccgggaa tgtatgtgcc gacgcaggcg tcgtggggcc
    21361 ataacaaccg caccgtcgcc ctgcgtattc cgtgcggcga ccgtcataat caccgcgtgg
    21421 aatatcgcgt ggcgggtgcc gatgccaacc catatctggt gatggcagcg atttttgccg
    21481 gtattttgca tggccttgat aacgagctgc cgttgcagga agaagtcgaa ggcaacgggc
    21541 tggaacagga aggcttaccc ttcccgattc gccagagcga tgccctgggt gagtttatcg
    21601 agaatgatca cctgcgccgc tatttaggca aacggttttg ccatgtgtat cacgcctgta
    21661 aaaatgatga gctgttgcag tttgagcgcc tcatcacaga aacagaaatt gagtggatgt
    21721 tgaaaaacgc gtaattcccc ttgcggggtt gtttcatcgg cctgagcgga gccaggcctg
    21781 acatttgctg gtaatccaga catttcccgg atgtcgcgtt atggagcgca ggcggcaacg
    21841 ggcttccgtt aacgattaag actgtgacga ggaaatgaga tgatgccgat gattaaatcc
    21901 ccgcatggcg agggaggctg cgtatgcgcc cctcctgcta ctgactggac tccgccccct
    21961 ttgcttccct tgctaaacag gtttgatttc aggtcgacac gaccgcaaac cttattacgc
    22021 aggggaggca gcaattatgg ctattaattc accactgaat attgctgcgc aacccggcaa
    22081 aacccgtctg cgaaaatcac tgaaattgtg gcaggtggta atgatgggtc tggcctatct
    22141 ctcgccgatg accgtatttg atacttttgg tattgtctcc ggcattagcg acggtcacgt
    22201 tccggcgtcc tatttgctgg cgctggcggg cgtgctgttt accgctatca gctacggcaa
    22261 actggttcgc cagtttccgg aggccggttc ggcctatacc tacgcgcaaa agtcgattaa
    22321 cccgcacgtc ggatttatgg tcggctggtc atcactgctg gattatctct ttttgccaat
    22381 gatcaacgtt ttgttggcga aaatctatct ctccgccctc ttcccggaag tgccgccgtg
    22441 ggtgtgggtg gtaaccttcg tcgccatttt aaccgccgcg aatctgaaga gcgtcaacct
    22501 ggtcgctaac ttcaataccc tgtttgtact ggtgcaaatc tccatcatgg tggtgtttat
    22561 cttcctggtg gttcagggac tgcataaagg agaaggcgtt ggcaccgtct ggtcacttca
    22621 gccgtttatc agcgagaacg cgcacctgat cccgattatt accggtgcga cgattgtctg
    22681 tttctcgttc ctcggtttcg atgcggtgac cacgctttcg gaagagacgc cggacgccgc
    22741 acgcgtgatc ccgaaagcca tcttcctgac ggcggtatac ggcggcgtta tctttatcgc
    22801 ggcgtcgttc tttatgcagc tgttctttcc cgatatcagc cgctttaaag acccggacgc
    22861 cgcactgcct gaaattgcgc tgtacgtcgg cggcaagctc tttcagtcga ttttcctctg
    22921 caccacgttt gtgaacacat tagcgtctgg cctggcttca catgccagcg tgtcgcgtct
    22981 gctgtatgtg atggggcgtg acaatgtgtt tccggagcgc gtgtttggct atgtgcaccc
    23041 aaaatggcgg actccggcac tcaacgtcat tatggtcggg attgtcgcgc tgtcggcgct
    23101 gttctttgat ttagtcaccg cgacagcact gattaacttc ggtgctctgg tggcgtttac
    23161 cttcgtgaat ctgtcggtgt ttaatcattt ctggcggcgt aaaggaatga ataaaagctg
    23221 gaaggatcac ttccactatt tgctgatgcc gctggttggc gcgctgacgg tgggtgtgct
    23281 gtgggttaac ctagagtcaa cgtcactgac actcggtctg gtatgggctt cgttaggcgg
    23341 cgcatatttg tggtatctga tccgccgcta tcgcaaagtg ccgctgtacg aaggtgacag
    23401 aacgccggtg agcgaaacgt aatagccgga tgcgcggcgc attatcctgc ctgagggtaa
    23461 cgcgccgcgc cattaactat aaaaagatgt atgacccgga tcacgagtgc tatcatccgc
    23521 ccttatgctt agcgcattaa ccccaggggc aaataatgaa ccacgatatc ccgctaaaat
    23581 attttgatat tgccgatgag tacgcgaccg aatgcgcaga gcctgtcgca gaggctgaac
    23641 gcacaccgct ggcccactac ttccagttac tgctcacccg tttgatgaac aatgaagaga
    23701 tcagcgaaga agcccaacat gaaatggctg ctgaagcggg gattaatccc gtacgcattg
    23761 atgagatcgc agagtttttg aatcaatggg gtaatgagta attatttaat tgcagacgat
    23821 cggtcagtat cgttgtccgg ataaagcgtt cacgccgcat ccggctaaca atgacacgca
    23881 ctgcagcgtg ctttaatggg tttctcacga tcgtaccgct ctgctcctcg ctaagttgtt
    23941 ctgtgagtta tagttataga gaaatcacgg gtacgaatgt tgaacagatg gcgacgccac
    24001 ctgttcaacg accaaccaca cggccctcat tacgccccct gaacacaaac ggtaatggca
    24061 aatgtcatta aatattagta agatagcctc tttacataaa atcccttaat attatgcgcc
    24121 aggtattatc gtctcttttg gtgattgctg gacttgtgag tggtcaggca atcgccgcgc
    24181 ctgaatcccc cccgcatgct gatatccgcg acagcggttt tgtctattgc gtcagcgggc
    24241 aagtcaacac ctttaaccca tccaaagcga gcagtgggtt aattgtcgat acccttgccg
    24301 cccagtttta tgatcgactg ctggatgtcg atccctatac ctatcgcctg atgccggaac
    24361 ttgccgaaag ctgggaagta ctcgacaacg gcgcgaccta tcgcttccac ctgcgtcgcg
    24421 atgttccgtt tcaaaaaacc gactggttta ctcccactcg taaaatgaat gccgacgatg
    24481 tggtgtttac cttccagcga atttttgacc gcaacaaccc gtggcataac gtcaacggca
    24541 gcaacttccc ctacttcgac agcctgcaat ttgccgataa cgtgaaaagc gtccgcaaac
    24601 tggataatca taccgttgag ttccgactcg cccagccgga tgcttctttt ttgtggcacc
    24661 tcgcaaccca ttatgcttcg gtcatgtcgg cagaatatgc ccggaagtta gagaaagaag
    24721 atcgccagga gcaactcgac cgtcaaccgg tcggcaccgg tccgtatcag ttgtcggaat
    24781 accgcgccgg gcaatttatt cgcctacaac gtcatgatga cttctggcgc ggtaaaccgt
    24841 taatgccgca ggtagtggtg gatttaggct ccggcggcac cggacgtctg tcgaaactcc
    24901 tgaccgggga atgcgacgtt ctggcctggc ctgctgccag ccagctatcc attttgcgtg
    24961 acgacccgcg cttgcgttta acgctgcgtc ctgggatgaa cgtcgcctat ctggcattta
    25021 acaccgccaa accgccgcta aataatcccg ctgtccgcca tgcactggca ctggcgatta
    25081 ataaccagcg cctgatgcaa tccatctatt atggtacggc tgaaacggcg gcctctattt
    25141 taccgcgcgc ctcgtgggcc tatgacaacg aggctaaaat tactgaatac aatccggcga
    25201 aatcgcgcga acagttgaag gcgttggggc tggaaaactt aacgctgaaa ctgtgggtgc
    25261 ccacacgttc gcaggcgtgg aaccccagtc cactgaaaac tgccgaactg attcaggcgg
    25321 atatggcgca ggttggcgta aaagtggtga ttgtgccggt agaaggtcgc tttcaggagg
    25381 cgcggttgat ggatatgagc catgatctga cgttatccgg ttgggcgacg gacagtaacg
    25441 acccggacag tttcttccgt cctttactga gctgcgcggc aattcattca cagaccaacc
    25501 tcgcccactg gtgcgatccg aaattcgaca gcgtgttgcg taaggcgctc tcctcgcagc
    25561 agctggcggc gcgtattgaa gcctatgacg aagcgcagag tattctggcg caggaattac
    25621 ctattctgcc gctggcgtcg tcattgcgtt tgcaggccta tcggtacgat atcaaaggtc
    25681 tggtacttag cccgtttggt aacgcctcct ttgctggggt gtatcgcgag aaacaggatg
    25741 aggtgaaaaa accatgatta tcttcacctt acgccgcatt ttgttattga ttgtcacctt
    25801 gttcctgctg accttcgttg gcttcagctt gagctatttc acccctcatg cgccgttaca
    25861 aggcgcgtca ctgtggaatg cctgggtgtt ctggtttaac ggcctgatcc actgggattt
    25921 tggtgtttcc agcattaacg gtcaaccgat tgccgaacag ttaaaagagg tcttcccggc
    25981 gacgatggaa ctgtgcatcc tcgccttcgg ttttgcgctg attgttggca tccccgtggg
    26041 gatgattgcc gggattacgc gccataagtg gcaggataac ttgatcaacg ccatcgcctt
    26101 actgggcttt tcaatcccgg tgttctggct ggcgcttctg ttgacgctgt tttgttcact
    26161 cacgctaggt tggttgcccg tttcagggcg tttcgatctg ctctacgaag tgaaaccgat
    26221 taccggtttt gcgttgattg atgcctggct ttcggactca ccatggcggg atgaaatgat
    26281 catgagcgca atccgccata tgatattgcc cgtgatcacc ctgtcggttg cgccaacaac
    26341 tgaagtgatc cgcctgatgc gtatcagcac tatcgaagtg tatgaccaga actatgtaaa
    26401 agcggcggcg acccgcggtt tgtcgcgctt tactattttg cgtcgccacg ttctgcataa
    26461 cgcgttaccg ccggttattc ctcgcctggg cttacagttt tccaccatgc tgacgctggc
    26521 gatgatcacc gaaatggttt ttagctggcc gggtttaggg cgctggttaa ttaacgccat
    26581 ccgccagcag gattacgcag ccatttccgc cggagtgatg gtgtgtggct cactggtcat
    26641 tattgttaac gtgatttctg atattttggg tgccatggct aaccctctga aacataagga
    26701 atggtatgcc ttacgatagc gtatacagcg aaaagcgccc gccgggcacg ctgcgtaccg
    26761 cctggcgcaa attttatagt gacgcctctg cgatggtcgg cctgtacggc tgcgcggggc
    26821 tggctgtact gtgtattttt ggcggctggt ttgcgcctta cggcatcgac cagcaatttc
    26881 tcggttatca attattgccg ccatcatggt cgcgctatgg cgaagtttct ttcttcctgg
    26941 ggactgacga cctcggacgc gatgttttaa gccgtttact gagcggagct gcgccaaccg
    27001 tgggtggcgc atttgtggtg acgcttgccg cgacgatctg tggcctggta ctgggcacct
    27061 tcgccggggc gacacacggc ctccgctcag cggtgcttaa ccatattctg gataccttac
    27121 tggcgatccc ttcgctgctg ttggcaatta tcgttgtagc gtttgccgga ccgagtttgt
    27181 cccacgctat gtttgctgtc tggctggcat tgctgccgcg tatggtacgt tcgatttaca
    27241 gcatggtgca tgacgaactg gaaaaagagt acgttatcgc cgcccgtctg gatggcgcat
    27301 caacgctgaa tattctctgg tttgctgtga tgccaaacat caccgccggg ctggtcaccg
    27361 agatcacccg cgcactgtcg atggcaattc tcgatatcgc cgcgctgggc tttctcgatc
    27421 tcggcgcaca actcccctcg cctgaatggg gagcgatgct cggtgatgcg ctggaactga
    27481 tttatgtcgc cccgtggact gtcatgctgc caggtgcggc aattatgatt agcgtgttgt
    27541 tggttaacct ccttggtgat ggcgttcgtc gtgcgattat tgcgggggtg gaataatgcc
    27601 attactcgat attcgtaacc tgaccattga atttaaaacc ggtgatgagt gggttaaagc
    27661 cgtcgaccgg gtaagtatga ccttaaccga aggtgaaatc cgcggtcttg ttggtgaatc
    27721 cggttccggc aagagtttga ttgcgaaagc gatttgtggg gtgaataaag ataactggcg
    27781 tgttactgct gaccgtatgc gttttgatga tatcgatttg ctgcgtctct ccgcacgcga
    27841 acggcgcaaa ctggttggcc ataacgtgtc gatgattttc caggaaccgc agtcgtgtct
    27901 tgacccttca gaacgtgtgg gtcgccagtt gatgcaaaac atcccagcct ggacctataa
    27961 aggccgttgg tggcagcgtt ttggctggcg caaacgccgt gccattgaac tgctgcaccg
    28021 cgtggggatt aaagatcaca aagatgcgat gcgcagtttt ccctatgagt tgaccgaagg
    28081 tgaatgtcag aaagtgatga tagccattgc actggcgaat caaccgcgtc tgctgattgc
    28141 tgacgaaccg accaactcaa tggagccaac aacccaggcg caaatctttc gcctgctgac
    28201 gcgtctcaac caaaacagca ataccactat tttgcttatc agccatgact tacaaatgct
    28261 tagccaatgg gcggataaaa ttaacgtgct ttactgcggt caaacagtgg aaaccgcgcc
    28321 gagtaaggag ttggtgacga tgccacatca tccttatacc caggcgctga tccgcgcgat
    28381 accagacttc ggcagcgcga tgccgcataa aagtcgcctc aatacgctgc ccggcgctat
    28441 cccactgctg gaacagttac cgattggttg tcgtctgggg ccacgttgcc cgtatgcaca
    28501 acgagaatgc attgtgacgc cacgtttgac gggggcgaaa aatcatctct atgcctgtca
    28561 tttcccgctg aacatggaga aagagtgaga tgatcgaaac gctgcttgaa gtgcgtaatt
    28621 taagtaaaac gttccgctac cggaccggct ggtttcgtcg tcagaccgta gaagcggtaa
    28681 aacccttgag ctttacgcta cgtgaaggcc agacactggc gattattggc gagaatggtt
    28741 cgggtaaatc cacgctggca aaaatgctgg cgggaatgat agagcctacc agcggcgagt
    28801 tattgattga cgatcatcca ctgcattttg gcgattattc cttccgcagt cagcgtattc
    28861 gcatgatttt tcaggatcct tcgacctcgt tgaatccccg tcaacgtatc tcgcaaattc
    28921 tggattttcc actgcgcctg aacaccgacc tggaaccgga acagcgtcgc aaacagatta
    28981 ttgaaacgat gcgtatggtt gggctgctac cggatcacgt cagttactat ccgcatatgt
    29041 tggcacccgg acaaaaacag cgtctgggtc tggcgcgcgc gctaatattg cgcccaaaag
    29101 tcattattgc cgatgaagcc ctcgcttcac tggatatgtc gatgcgttcg cagttgatta
    29161 atctgatgct ggagttacag gaaaaacagg gcatttcgta tatttatgtt acccagcata
    29221 tcggaatgat gaagcacatt agcgaccagg tgctggtgat gcatcagggc gaggttgtcg
    29281 agcgaggcag caccgcggat gtgctggcat cgccgctgca tgaactcacc aaacggctga
    29341 ttgccgggca ctttggcgaa gcattgacag cggatgcgtg gcgtaaagat cgctaatttt
    29401 ctgaatcctc cttccagatt atccacctca ttctgacaag agattaaatc ttgtcagaat
    29461 gaggtgatgg ataaaataaa atcaaatgcg cgtgatttaa gacgcaatct cactttgcag
    29521 gaacgaaagc tctggcgata tcttcgcagc cgacgttttg gtgatttcaa atttcgccgt
    29581 caacatccgg tggggagcta cattctcgat tttgcttgct gctcggcgcg cgtagtcgtt
    29641 gagctggatg gtgggcagca tgatttggca gttgcctatg ataccaggcg cactagctgg
    29701 cttgagtcgc agggctggac cgtgctgcgt ttctggaata acgagattga ttgtaatgag
    29761 gaggcggtgc cggagattat tctgcaggaa ctgaaccgcc gatcaccctc accctgaaag
    29821 ggcgaggggg ccgaccgagc cgaatagctg ttgtggtgaa aacatggaga cagtgctgga
    29881 gaatattcgg caaggtctga accatcccag ccatcgccat aaaagggtta ggggctgtat
    29941 gagcctgttt gttgctgggg taacaatatt tgcacaatat ggtcccctcg cccctctggg
    30001 gagagggtta gggtaagggg aaaagcgccc accctgccgc agcctgctcc ggtcggacct
    30061 ggcaactata gctactcaca gccaggttga ttataataac cgtttatctg ttcgtactgt
    30121 ttactaaaac gacgaatcgc ctgattttca ggcacaacaa gcatcaacaa taaggattaa
    30181 agctatgggt tttctttccg gtaagcgcat tctggtaacc ggtgttgcca gcaaactatc
    30241 catcgcctac ggtatcgctc aggcgatgca ccgcgaagga gctgaactgg cattcaccta
    30301 ccagaacgac aaactgaaag gccgcgtaga agaatttgcc gctcaattgg gttctgacat
    30361 cgttctgcag tgcgatgttg cagaagatgc cagcatcgac accatgttcg ctgaactggg
    30421 gaaagtttgg ccgaaatttg acggtttcgt acactctatt ggttttgcac ctggcgatca
    30481 gctggatggt gactatgtta acgccgttac ccgtgaaggc ttcaaaattg cccacgacat
    30541 cagctcctac agcttcgttg caatggcaaa agcttgccgc tccatgctga atccgggttc
    30601 tgccctgctg accctttcct accttggcgc tgagcgcgct atcccgaact acaacgttat
    30661 gggtctggca aaagcgtctc tggaagcgaa cgtgcgctat atggcgaacg cgatgggtcc
    30721 ggaaggtgtg cgtgttaacg ccatctctgc tggtccgatc cgtactctgg cggcttccgg
    30781 tatcaaagac ttccgcaaaa tgctggctca ttgcgaagcc gttaccccga ttcgccgtac
    30841 cgttactatt gaagatgtgg gtaactccgc ggcattcctg tgctccgatc tctctgccgg
    30901 tatctccggt gaagtggtcc acgttgacgg cggtttcagc atcgctgcaa tgaacgaact
    30961 cgaattgaaa taatcattct gttggtaaat attgggcggc gttctgccgc ccgttatctc
    31021 tgttatacct ttctgatatt tgttatcgcc gatccgtctt tctccccttc ctgccttgcg
    31081 tcaggataac gatttccttt acgaccaagg agcgcccatg gaacaacgcc acatcaccgg
    31141 caaaagccac tggtatcatg aaacgcaatc cagtactgcg gagtatgacg ttctgcctct
    31201 ggtcccggaa gccgcaaagg tcagcgatcc ctttctgctc gacgtgatcc ttgatgaaga
    31261 aacgctggct cccttccttt catggctggt ccctgcgcgc gttcttgcag tggaattgtt
    31321 ccctgaccag cttaccgtga cccgttcaca gaccttcacc gcttatgaac gcttgtctac
    31381 ggccctgacg gttgctcagg tttgcggcgt ccagcggtta tgtaactact attcggcgcg
    31441 acttacgccg ctccccgggc ctgattcctc cagggaaagc aatcataggt tggcacaaat
    31501 cacgcaatat gcccgccaac tggctagctc gccttctatt atcgacaacc gatcgcgcca
    31561 gcatctgaat gacgtcggtc ttactgcctg ggactgtgtg atcattaacc aaatcattgg
    31621 ttttattggc tttcaggcgc ggaccattgc gacatttcag gcttatctcg ggcacccggt
    31681 acgctggtta cccggtctgg aaatacaaaa ctacgccgac gcgtcactgt ttgctgatga
    31741 atcaatacgc tggcgaagca gctatgaggt ggaaaaacta cctgaagagt acacaaaaag
    31801 ttcaacagca gaactttgcc aactggccga aacactctct ctccacccta tttcactttc
    31861 ccttctcgaa aggttgttaa acagtacacg ggttaataca cagccggata ataagcttgc
    31921 ggcgttgtta tgcgcgcgga taaatggcag tcctgcttgt tttgccgcct gtatggattc
    31981 atcaaatgaa tataaaaaat cagccccctt ctgcgcaagg gcgaaaatga aattaaccaa
    32041 tgggctgacc gtcattctgt tgagcacgct accgttcagg cgatacaatg gctgacccga
    32101 gcacccgatc gctttagcgc cgcccagttc agccctttac tcgaacacga aaaatcatca
    32161 acgcagatta ttaatctgct ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa
    32221 atcgcgttgg gtgagacata ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca
    32281 gccgctctaa tggccaccaa aatagacaat tatgtttcag gacaacccgc tgctagcgca
    32341 gcttaaacag caactgcatt cccagacgcc acgcgctgaa ggggtggtaa aagccacaga
    32401 aaaaggcttt ggcttcctgg aagtcgacgc gcaaaaaagt tatttcattc cgccgccgca
    32461 gatgaaaaaa gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg aaaaagaacg
    32521 tgaatccgca gagccagaag aactggttga accgttcctg actcgtttcg tgggtaaagt
    32581 tcagggcaaa aatgaccgtc tggccatcgt tcctgatcat ccactcttaa aagacgccat
    32641 tccttgccgc gcagcccgtg gcctgaacca cgagtttaaa gaaggcgact gggcggttgc
    32701 cgaaatgcgc cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac tgacacaata
    32761 catcactttt ggtgacgacc actttgtacc gtggtgggtt acccttgcgc gccataatct
    32821 ggaaaaagaa gcaccagacg gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga
    32881 agatctgacc gcgctggatt ttgtcaccat cgacagtgcc agcacagaag atatggatga
    32941 cgcccttttc gctaaggcgt tgccggatga caaacttcag ctgattgtgg cgattgccga
    33001 tccaaccgcg tggattgctg aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt
    33061 caccaactat ctgcctggct tcaacatccc tatgcttcct cgcgagcttt ctgacgatct
    33121 ctgctcactg cgcgccaatg aagtccgccc ggtactggca tgccgcatga cgctctccgc
    33181 tgatggcacc attgaagata atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa
    33241 gctggtgtat gaccaggttt ctgactggct ggaaaatacc ggtgactggc agcctgaaag
    33301 tgaagcaatt gccgaacaag tccgtttgct agcgcaaatt tgccaacgcc gcggcgagtg
    33361 gcgtcataac cacgcactgg tgtttaaaga tcgcccggat taccgcttta ttctcggtga
    33421 aaaaggtgaa gtgctggata tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga
    33481 agaagcgatg attgccgcta acatttgtgc ggcccgcgtt ctgcgcgata agctcggttt
    33541 tggcatctat aacgtgcata tgggctttga tccggcgaat gccgacgcgc tggcagcgtt
    33601 gctgaaaacg cacggtctgc atgtcgatgc cgaagaagtg ctcacgctgg acggtttctg
    33661 caaactgcgt cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg
    33721 cttccagtca tttgctgaaa ttagcactga acccggtcct cactttggcc tcggtctgga
    33781 agcatacgcc acctggactt cgccgatccg taaatatggc gacatgatca accaccgtct
    33841 gctgaaagcg gttatcaaag gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca
    33901 aatggccgaa cgtcgccgtc tcaaccggat ggcagaacgt gatgttggcg actggttata
    33961 cgcacgcttc ctgaaagata aagccgggac cggcacccgt ttcgcggcgg aaattgtcga
    34021 tatcagccgt ggcggcatgc gtgtgcgttt ggttgataac ggcgctatcg cctttattcc
    34081 ggcacctttc ttacacgctg tgcgcgatga actggtttgc agccaggaaa acggcactgt
    34141 acaaattaaa ggtgaaacgg tttacaaagt aactgacgtt attgacgtca ccattgccga
    34201 agtccgcatg gaaacccgcg gcattattgc gcgcccggtc gcgtaatctc ctttcacggc
    34261 ccattcctca tggatgggcc gtttatttcc ccgctctacc ttcgttatat ccaggcagat
    34321 tttttaactg actttcgttt gaaaactggc gtttttccaa aactggatta caaataaata
    34381 tataaaaaaa caatgcgttt agccgccgtt aatttcatgt aagtcatgtt gttcctttta
    34441 tttaaccgcg gactccgcag ttaaccggag gatatgcatc atgaaaaccg ttagggagtc
    34501 cacaacgttg tacaactttc tcggatcgca caatccatac tggcggttga cggaaagcag
    34561 cgatgttttg cgcttttcta ccaccgaaac cacagaacct gatcgtactt tgcagttatc
    34621 tgccgaacag gctgctcgca tcagggaaat gacggtcatc acctccagcc tgatgatgag
    34681 tctgaccgtc gatgagagcg atctttctgt gcatctggta ggacgaaaaa tcaataaacg
    34741 ggaatgggct ggcaacgcgt ctgcatggca tgacacaccg gcagttgctc gtgatttatc
    34801 acacgggctt tcctttgctg agcaggtagt ttctgaagca cattccgcaa tagtgattct
    34861 cgacagccgg gggaatatcc aacgcttcaa tcggttatgt gaagattaca ctgggttgaa
    34921 agaacacgac gtcattggcc aaagcgtgtt taaactgttt atgagccgtc gtgaagctgc
    34981 ggcatccagg cgcaataacc gtgtattttt tcgaagcggc aatgcatatg aagtcgaact
    35041 gtggatacca acacgtaaag gccagcggct gtttctgttt cgcaataaaa taaatttgtc
    35101 cacagcggca gtggcaaaaa cgagattttt ttaatctgtt ccggtaccga cattaccgaa
    35161 gagcgccgcg ctcaggagcg actgcgtatt ctggcaaata ccgacagtat caccggactg
    35221 ccgaatcgta acgcaatgca ggagttaatc gatcacgcta ttaatcaggc agataacaat
    35281 aaagttgggg ttgtgtatct tgatttggat aattttaaaa aggtcaacga cgcctatggg
    35341 catttgtttg gtgaccagtt attacgcgac gtgtcattgg ctattttaag ctgtctcgaa
    35401 catgaccagg tgttggcgcg tccaggtggc gatgagtttc tggtactggc atccaatacc
    35461 tcacaaagcg cgctggaagc aatggcatca cgaattttga cccgcttacg cctccccttt
    35521 cgtattggtt taattgaagt ttataccagc tgttcagtag gtatttcgct ctctcccgaa
    35581 catggttcgg acagcgcgac tattattcgt cacgccgaca cagcaatgta cacagcgaag
    35641 gaaggcggac gaggacaatt ttgcgttttt actccagaaa tgaatcagcg ggtatttgaa
    35701 tatctctggc tggataccaa cttgcgtaaa gcactggaaa acgatcagtt ggttattcac
    35761 tatcaaccga aaatcacctg gcgtggcgaa gtgcgcagtc tggaagcact agtacgttgg
    35821 cagtcacctg aacgtgggtt gattccaccg ttggacttca tttcctacgc cgaagagtca
    35881 gggctaattg tgcctttagg ccgttgggtg attctcgatg tcgtacgcca ggtggcaaag
    35941 tggcgggata aaggcataaa cctgcgagtg gcggtaaata tttctgcacg tcagctcgcc
    36001 gatcaaacca ttttcaccgc cctgaaacag gttctccagg aactcaattt tgaatactgc
    36061 cctatagatg ttgaactgac agagagttgt ctgattgaga atgatgaact ggcactgtct
    36121 gttattcaac aatttagcca actaggtgcg caagtgcatc tggatgattt tggtaccggc
    36181 tactcttcac tttcgcaact ggcgcgcttt ccgatcgatg ccatcaaact tgaccaggtt
    36241 tttgttcgag atattcacaa acaacctgtc tcgcagtcac tggtccgggc gatcgtcgct
    36301 gtggcccagg cattgaatct tcaggtgatc gccgaaggtg tagagagtgc aaaggaagat
    36361 gcttttttaa ccaagaacgg gatcaatgag cggcaaggat ttttgtttgc caaaccgatg
    36421 cccgccgtcg ccttcgaacg ctggtataaa cgctatctga agcgcgcata agtagatgaa
    36481 tatatatact tagcgctata tcccgtcatt atatatcgta ttcctggatg ataattagcc
    36541 gttgctgtta aacacttctc ttttagcggc aatttttgca ttattcattt tccacttttg
    36601 ctataaggat gtccttgatg tctgaatttg atgcccagcg cgttgccgaa cgtattgata
    36661 ttgtattaga tatccttgtg gcaggcgatt accactctgc gatccataat ctcgagatac
    36721 ttaaagcaga actcttgcgc caggttgctg aatctacgcc agacatcccg aaggcaccgt
    36781 gggagattta acaatcgtgc cttcaggttt tcctatttga ggatgcccgg cgcgtcagta
    36841 tttttttatt tagtattata acgttataag aattacagcg atgaattccc gacaacaaac
    36901 tattctacag atggtcattg accagggtca ggttagcgta accgatctgg caaaagccac
    36961 tggagtttct gaagttacca ttcgccagga tctcaacacc ctcgaaaaac tgagttacct
    37021 ccgccgtgca catggctttg cagtttcgct tgatagtgat gacgtcgaaa cccgtatgat
    37081 gagcaactat acgctgaagc gtgaactcgc cgagtttgcc gcgtcactgg ttcaaccggg
    37141 cgaaaccatc tttatcgaaa atggcagcag caatgccctg ctcgcccgga ctctggggga
    37201 gcagaagaaa aatgtcacta tcatcacggt cagcagctac atcgcgcatt tgctgaaaga
    37261 cgcgccttgt gaagtcattt tgctcggtgg cgtgtaccag aaaaaaagcg aaagtatggt
    37321 tggcccgttg acacgccagt gcatccaaca ggtgcatttc agcaaagcat ttattggtat
    37381 tgatggctgg caacctgaaa ctggattcac cggtcgcgat atgatgcgta ccgatgtggt
    37441 caatgccgtg ctggaaaaag agtgcgaagc gatagtcctg actgacagct cgaaatttgg
    37501 tgctgtacat tcatactcca tcggtcccgt tgagcgattc aatcgcgtga ttaccgattc
    37561 gaaaatacgc gccagcgatc tgatgcatct tgagcacagt aaactcaccg ttcacgtcgt
    37621 tgacatttaa ccatttccct tctctacgga tgatttgcag tttggcaaat catccgctct
    37681 aagatgattc ctggttgata attaagacta tttacctgtt attaacacgc ttaagatata
    37741 aaattattat cagcgatata acaggaagtc attatcacct gcgtgatata accctgcgcg
    37801 cgagcagatt tcacggaata atttcaccag gcttattctt agctattata gttatagaga
    37861 gcttacttcc gtgaatcata aattcaggag agagtattat gtttgtaacg agcaaaaaaa
    37921 tgaccgcggc tgttctggca attactttgg caatgtctct gagtgcctgt tctaactggt
    37981 ctaaacggga ccgcaacacc gcaatcggcg cgggtgcagg ggcattaggc ggtgcagtac
    38041 tgaccgatgg cagtacgttg ggtacattag gtggtgcagc tgtcggtggt gttattggtc
    38101 accaggtcgg taaataatcg taaataaatg tcgattatcg actcgcaatt ataatacgct
    38161 tgattctgtt cagtacgaaa taactgaata atacgcacag taaaatattc gaaagccacg
    38221 gatatatccg tggcttttta tgttaaccgc ctgcgagttt taccttcatc cctttcgctt
    38281 caagtagtga ttttaataaa tcacgcttat cgccctggat ttcaataact ccatctttaa
    38341 ctgctccgcc gcagccgcat tttttcttca gttccgctgc aagttttgtc agttcggcat
    38401 catcgagatc gacaccggta atcaggcaaa cgcccttacc tttacgtcca ctggtctgac
    38461 gctgaatacg caccacaccg tcgcctttag ggcgtacagg ggccgctttg ggctcatcaa
    38521 tacgtccggt ttccgttgag tagaccagac ggctgttgga atcactcatc atgcactccg
    38581 ctgtaaagag gcgttgatcg ctttcagcgt ctgcgctgga tctaccgatt gcgttaccgg
    38641 gcgaccaatc accatataat caacaccagc cgacaacgcc tgttctggcg tcataatgcg
    38701 gcgctggtca ccagcttcac tcccctgcgg acgaatgccc ggcgtaacca gtttgaactc
    38761 ctgaccgaat acctgtttaa agcgcacagc ttcctgagca gaacacacca caccatcaag
    38821 gccacatttt tgcgtcagtg ccgccagacg ttctgcataa tctgcaggtg acagtgtcat
    38881 gccaagatcg accaggtcgc tggcttccat gctggtcaac actgtcacag caatcaaaag
    38941 cggtgcatct ttgccaaacg gaaccagtgc ctcacgcgct gcggtcatca tacgcgcccc
    39001 accagaggca tgaacattca ccatccacac gcctaagtca gctgcagcag cgacagcgtg
    39061 cgctgcagtg ttaggaatat cgtggaattt caggtcaaga aagatatcaa aaccacgctg
    39121 ttgaagttcg cgcacaaact gtggcccaaa caatgtaaac atctctttgc cgaccttcag
    39181 acgacaatcg cgtgggtcga tcttgtcgac aaaggacagc gcgtcatcac gattatgata
    39241 atcaagggca acaaccacag gagaattcgt aacagcgcgg gaagaagatg aagcagttaa
    39301 cgtcatgacc agaccttctt gatgatgggc accggaagtg gcgcgaaaca ggtaaacggc
    39361 gagcattcta cccgtggaaa taaaaaatta acagttgcga tttttcccct ggcaggcttt
    39421 ttctgctccg tcgttgccgg agcacaatgg aacatcaata actagtatgt tgtaactaaa
    39481 gtcggatttt tttaaaaatt acaggccatc aagaccgcga atcggtttaa tggttgacca
    39541 ggcccgacaa gacggacaat gccagtagag ggtgtatgcg gtaaaaccac atttctggca
    39601 gcgataacga ggcttactac gtaccttttc gccaaccatg tcacgcagca ccatcaggct
    39661 ctctttggca cgcccttctt ccgcttcatt taagtggtaa tccattaact tatggaacac
    39721 acgcatggtc ggatgacgct gaagctggcg cgtaatatag acctgtgcgg cctcactacc
    39781 gtcgcgcgct tcgatgatat cagcaagcat caattcagca tcggcaccgg tgttctcttc
    39841 caccgcgcgt tgcaggaatt ctgcccattc ggcagtttta cccaactgct gatagcaggt
    39901 ttgcaacatt tccagcgttt cgctgaccag ttctctgtcc tgggatatca cgcgttgcaa
    39961 actttcgacg gctttggcgt attctccttt cgccataaac acgcgtccca tcatgatgga
    40021 tacgcgggcg ctgtttttat ctgccgccgc cccttttttt agcaaggtca tggcacgatc
    40081 gagatcgtcg ctggccatat gctgcagggc taactcacag tagaaatggg caatttcgac
    40141 gcgctgttta tctttaccca gcttcaccag gcgttcggca acatcaattg ctttctgcca
    40201 ctcgctggta gcctggtaga tttgtagcaa ctgttgcagc gcgccaatgc ggaagtcagt
    40261 ttcatcggtc agctgattga acatgtcttc cgcgcggtcg tataacccgg cggccatgta
    40321 atcacgcccc agttgttgaa tcgccaacag acgctgttca taggtcagtg aggcgctttc
    40381 cattagggtc tgatggatgc gaatagcgcg atcaacttcg ccacgcgaac ggaacaggtt
    40441 tccgagcgta aggtgggctt caacggtgcc tgtatcctct ttaagcatat cgagaaacag
    40501 gtctaccgct ttatcctgtt gattactaag caggaagtta acccccgcta cgtaatcacg
    40561 cgacaagcgg ttggcttcat cttgcttgtt ttgttgcgca cttctgcggc ccatatacca
    40621 gccataggca gcggctacag gcaaaagcag aaacaacaac tccagcatag aaagttattc
    40681 cttcgccgct gacgagtgcg gcactacagc cacgtcagtc gcgggtgaaa gctggttttc
    40741 cagtcgcttt attttacgtt cagcgcgcgc cagggaaaca cgaactcgca gccagaacag
    40801 gccacaaatc aaccaaccga tagcaacccc cgcagcaaac aataccgcca gcaatgtgga
    40861 aatacggtac tccccttgcg ctaacagata attaaacgtc acctgttgat cgttctgcgc
    40921 acccaacgtg accgaaatca cgaagatcgc taacaccagt aagaaaatga gtaaatattt
    40981 cacattactt cccgttatgt ggttaaagcg aataaattat ggtcaactta ccgcaatagc
    41041 cctttaaagt accattttcg cgccgtctgc gaaatggcat ccacgcgcat tgttgatgat
    41101 ttaagggcta aattcggaaa atcaaccagc gttaactttc ttgttctcgt tgcgctattt
    41161 cgcgattttc ttccgcaggt ggtgttaatg gcccacaaat tcgttgcgca agccacgttg
    41221 ccaccgccac cagcgcccac gaaatcaacg tagctactac cagatcgcgt ggccaatgca
    41281 tcccgagcag caggcggctt cccatgactc ccgttgccca gaccagcaag atagcaatgg
    41341 ttaacgttcg ccgacgcggc cacagcaaac caacggccag cagtgcccaa ctggcagcaa
    41401 acatcgtgtg accggaagga aaggcaaacc ccgtctcttt ctgccagtgt gaacgcaaat
    41461 attgtgggat atttttctct tcagccaact gttctttcac tagatttccg cgttctgctc
    41521 gctttaaagt gtagaactca tcaaccggaa tatgatgtgt tttttccagc cagataacaa
    41581 aaggtcgtgg ttcctggact ttgtctttga tccaggattt aacgccttgt cccacaatga
    41641 ttacggccgc cagaatggca aataatacaa aggcagcctt aatgcgaaaa cgcagacacc
    41701 agagaaacca gccgaataaa atcaaatgtg taatgacgcc ccagggctgg gtgacagttt
    41761 cagtaaccca aaaagccgct tttagtagcc aactttgttc tccaggttgc caacgccagc
    41821 cagaaatcca tacggctact ggcatgacaa gcaatagtgc agctcccact gcggtacgtc
    41881 tggcaatcga acgcatggcc tctccttttt gataagtccc acaatcataa ctgaaaacgc
    41941 cagttccagg aaaaattgac agatttgtgc cattccgtga acgatcgacg cgtcgtgatt
    42001 aggtgaaccc cttctcgtta tggcaaaata agccaataca gaaccagcat tatctggaga
    42061 atttcatgca gcttaaacgt gtggcagaag ccaaactgcc aaccccatgg ggcgatttcc
    42121 tgatggtggg atttgaagaa ctggcaaccg gacacgatca tgtcgcgcta gtctatggcg
    42181 atatttccgg gcataccccg gtacttgcgc gcgtccattc cgaatgtctg accggtgacg
    42241 ccctgttcag cttgcgctgc gattgtggct tccagctcga agcggcattg acgcaaattg
    42301 ccgaggaagg ccgtggtatt ttgctgtatc accgtcagga aggtcgtaac attggtctgc
    42361 tgaataaaat ccgcgcttac gcactgcagg atcaaggtta cgataccgta gaggctaacc
    42421 atcagttagg cttcgccgct gatgagcgcg acttcactct ttgcgctgat atgttcaaac
    42481 tccttggcgt caatgaagtc cgcttgttaa ccaataaccc gaaaaaagtc gaaattctga
    42541 ccgaagcagg gattaatatt gttgaacgcg taccattgat tgtaggtcgt aaccccaata
    42601 acgaacatta tctcaatacc aaagccgaga aaatgggtca tttgctgaac aaataaccct
    42661 ctcgcattgt ggaattaatt tgcttgccgg aagcaaaata accggcaagc aaatatttgt
    42721 tacttcagca tattacgaat gacataatgc aaaatgccgt cgttctggta gtaggtcaac
    42781 tccgtcgcgg tgtcgatacg acaacggcag ggtacgacct cctggctacc atccgcgcgc
    42841 gtaagcgtca ccggaaccgt cgcaccgggt tgtagatttt gcagatcgcc aatatcaatc
    42901 ttctcttccc cggttagtcc taacgtttta cgcgttacgc cttgcggaaa ttccagcggc
    42961 aggatgccca tgccaattaa attcgaacgg tgaattcgtt caaacgattc ggcaatcacc
    43021 acacgaattc caagcagacg aggacctttt gccgcccagt cacggcttga gcctgaacca
    43081 tactctttcc cggcaatcac cgccagcggc gtttgttcct gcttatagcg catcgcagca
    43141 tcataaatag agactacgtc gctgtcaggt aaatgccgcg tcatcccccc ttcaacgcca
    43201 ggcaccattt cattacggat gcgaatattg gcgaaggtgc cgcgcatcat cacttcatgg
    43261 ttaccacgcc gcgaaccgta ggagttaaag tcttttcgct caacaccccg accttgtaga
    43321 tatcgacccg ctgggctgtc gggcttaata ctgcccgccg gagagatatg gtcagtggtg
    43381 actgaatccc ccagcattgc gaggatccgc gcaccgtgaa tatcttccac tggtgctggt
    43441 gttgcctgca tttcatcaaa gaaaggcgat aagcgaatat aggttgagtc ctcctgccaa
    43501 ccgtaggtat cggatcgtgt gacgttaatt tccttccact ctgctgtgcc ttcaaaaact
    43561 tctgcgtact ctttgcggaa catttctgtg gagacttgtt ctaccgcacg ggcaatttct
    43621 tgtgccgatg gccagatatc tttcagataa accggatcgc ctttacgatc atgaccaata
    43681 ggctcagaag ccaggttgat attcatattt cccgccagcg cataggcaac caccagcggc
    43741 ggcgaggcca gccagttggt tttaaccagc ggatggatac ggccttcaaa gttacggttg
    43801 ccggacagca ctgcaccgac ggttaaatcg ccttttttga ttgccgtttc gataggatcg
    43861 ggcagcggcc cagagttacc aatacaggta gtacaaccgt atcccacaag gttaaacccc
    43921 agttcgtcga gaaacggtgt cagtttcgct tttgccagat agtcagaaac aactttcgag
    43981 cctggtgcca gcgacgcttt gacccacggt tgccgcttga ggccaagaat tacggctttt
    44041 ttcgccagca agcctgcagc catcagcaca cttgggttag aggtgttggt gcacgaggtt
    44101 atcgcagcaa tgaccacagc gccatcaggt aactgatact gatgtccgtt cataacataa
    44161 tcgaccggct ggcgatcttt atgcgtggca ttcacttcca gttcgttact ggcggcaaat
    44221 acttttggta catcgggcag tgcaacgcga tcctgtgggc gttttggccc tgccaggctc
    44281 gcttcaacgt cattcatatc cagttctaac gtactggtaa aaatcggttc atcgcccggg
    44341 ttacgccaca tgccctgcgc tttggcatat ttttcgacca actcaacctg atcttcgctg
    44401 cgcccgctta aacgcatgta atcgagggtt acagcatcga ttgggaagaa gccacaggtg
    44461 gcaccatatt ctggcgacat attggcaatg gtggcgcgat ccgccagcgg tagtgaatcc
    44521 agaccatcac cataaaattc gacgaatttc tccaccacgc catgtttgcg cagcatttgg
    44581 gtaacagtga gaaccaggtc tgtggcggta ataccttcac gtaatttacc ggtaagttta
    44641 aagcccacta cgtccgggat aagcatggaa accggctggc ctaacattgc ggcttctgct
    44701 tcgatcccac ccacgcccca ccccagcacg ccaaggccgt tgatcatagt ggtgtgcgag
    44761 tcagtaccaa cgagtgtatc cggataagca atccattcac cgtcctgcaa ttcactccac
    44821 actgctttgc cgagatattc gaggttaacc tgatggcaaa tgcctgtgcc tggcggcacg
    44881 acgctaaacc gactgaacgc ttgctttccc catttcagga acacataacg ttcgtggttg
    44941 cgctccattt ccaggcgtac gttttcttca aatgcctcat catcaccaaa acgatcgacg
    45001 gtcaccgagt ggtcaatgac caggtcgacc ggtgagagcg ggttaacctt tgcagtatcg
    45061 ccgccgaggc gtttaaccgc ttcgcgcatt gccgccagat caacaacggc aggtacgccg
    45121 gtaaagtcct gcatcaacac ccttgccggg cggtaggcaa tttcacggtc agcatgggca
    45181 tttttcagcc atcctgccag cgcgtggata tcctcttcgg taaccgagtt accatcctgc
    45241 cagcgcagca ggttttcgag caaaactttg agtgacttgg gtagacgggt gatatcgccc
    45301 agtgatttag cagcaagcgg caggctgtag tagtggtaag ttttatcttt ggcctgcaac
    45361 gtgtccttac tggcttctcg tagggttgac gacatagctc ctccttaatg acagggttgc
    45421 gaagccctga ttctcttcag ggtgaatatt aaagataaca caaacatcgc gtaacgattt
    45481 gataacaacc caaattgata aaagaggaga aaccttgtgg caaagcaaat gacaaccccg
    45541 ccgcagcggg gtcaaggatc agtgagtaat gatccagagg agttcggccc agaagagcat
    45601 cgacagagaa aacgcgccca tccaggacca atatttcata tttgtgttga tcataggcta
    45661 cacccttaac ggttattatt ttgtgttgag ataactcaag ctgttattgc tgcggtgtat
    45721 taaaagccct ttaattcatt aaaaatgggt aataagaatg atgataatta ttgtttcggc
    45781 tgatcatctt cagcaaacag ttctataaag gcttgctgct gcgatgttag ttgccagtcg
    45841 tctggaataa cgacggaaca ttcccgtttc gtttctgttt cattgtcact atgctggcat
    45901 aatggtgacg actccagttg ctcctgaccg accatagtta tccccacact ttccaaatca
    45961 gcagtgcgac aaccacccag aaaagtgcag aaccaagaaa aaccgccagc caggctttac
    46021 gttttaattc aggatcgcgg cggggttcct gatttccaga aggcattgct aacctcatat
    46081 aatcgccatc acttatcagc aagacgttga acgattggtg taattaatca tacgatgaga
    46141 caaatcctaa agaaacttta gt
//
LOCUS       DAESHI010000021        45983 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.021, whole genome shotgun sequence.
ACCESSION   DAESHI010000021 DAESHI010000000
VERSION     DAESHI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45983
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.021"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(368..712)
                     /gene="tdcR"
                     /locus_tag="KW011_001220"
     CDS             complement(368..712)
                     /gene="tdcR"
                     /locus_tag="KW011_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HBH7659612.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            901..1839
                     /gene="tdcA"
                     /locus_tag="KW011_001221"
     CDS             901..1839
                     /gene="tdcA"
                     /locus_tag="KW011_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HBH7659613.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            1938..2927
                     /gene="tdcB"
                     /locus_tag="KW011_001222"
     CDS             1938..2927
                     /gene="tdcB"
                     /locus_tag="KW011_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HBH7659614.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            2949..4280
                     /gene="tdcC"
                     /locus_tag="KW011_001223"
     CDS             2949..4280
                     /gene="tdcC"
                     /locus_tag="KW011_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HBH7659615.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            4306..5514
                     /gene="tdcD"
                     /locus_tag="KW011_001224"
     CDS             4306..5514
                     /gene="tdcD"
                     /locus_tag="KW011_001224"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HBH7659616.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            5548..7842
                     /gene="tdcE"
                     /locus_tag="KW011_001225"
     CDS             5548..7842
                     /gene="tdcE"
                     /locus_tag="KW011_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HBH7659617.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            7856..8245
                     /locus_tag="KW011_001226"
     CDS             7856..8245
                     /locus_tag="KW011_001226"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HBH7659618.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            8317..9681
                     /gene="tdcG"
                     /locus_tag="KW011_001227"
     CDS             8317..9681
                     /gene="tdcG"
                     /locus_tag="KW011_001227"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBH7659619.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            9956..11287
                     /locus_tag="KW011_001228"
     CDS             9956..11287
                     /locus_tag="KW011_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBH7659620.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGAFYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            11315..12622
                     /locus_tag="KW011_001229"
     CDS             11315..12622
                     /locus_tag="KW011_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBH7659621.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAVAAAELE
                     GPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQA
                     IADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNGV
                     VFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEGL
                     SGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQG
                     ITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAAG
                     MAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDTA
                     VTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(12756..12920)
                     /locus_tag="KW011_001230"
     CDS             complement(12756..12920)
                     /locus_tag="KW011_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659622.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(12943..13644)
                     /locus_tag="KW011_001231"
     CDS             complement(12943..13644)
                     /locus_tag="KW011_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HBH7659623.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            13766..13948
                     /locus_tag="KW011_001232"
                     /pseudo
     CDS             13766..13948
                     /locus_tag="KW011_001232"
                     /inference="COORDINATES: protein motif:HMM:NF012354.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            13949..14641
                     /locus_tag="KW011_001233"
                     /pseudo
     CDS             13949..14641
                     /locus_tag="KW011_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004994521.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(14692..15048)
                     /locus_tag="KW011_001234"
     CDS             complement(14692..15048)
                     /locus_tag="KW011_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBH7659624.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(15290..15655)
                     /locus_tag="KW011_001235"
     CDS             complement(15290..15655)
                     /locus_tag="KW011_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBH7659625.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(15920..16933)
                     /gene="yqjG"
                     /locus_tag="KW011_001236"
     CDS             complement(15920..16933)
                     /gene="yqjG"
                     /locus_tag="KW011_001236"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HBH7659626.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYISLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESTEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPQDEMFASVKKRHLT
                     KCL"
     gene            complement(17003..17395)
                     /locus_tag="KW011_001237"
     CDS             complement(17003..17395)
                     /locus_tag="KW011_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBH7659627.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(17581..17880)
                     /locus_tag="KW011_001238"
     CDS             complement(17581..17880)
                     /locus_tag="KW011_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HBH7659628.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(17870..18274)
                     /locus_tag="KW011_001239"
     CDS             complement(17870..18274)
                     /locus_tag="KW011_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBH7659629.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(18277..18582)
                     /locus_tag="KW011_001240"
     CDS             complement(18277..18582)
                     /locus_tag="KW011_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBH7659630.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(18620..18988)
                     /locus_tag="KW011_001241"
     CDS             complement(18620..18988)
                     /locus_tag="KW011_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HBH7659631.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(19135..19518)
                     /gene="mzrA"
                     /locus_tag="KW011_001242"
     CDS             complement(19135..19518)
                     /gene="mzrA"
                     /locus_tag="KW011_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HBH7659632.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(19522..20184)
                     /locus_tag="KW011_001243"
     CDS             complement(19522..20184)
                     /locus_tag="KW011_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBH7659633.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(20529..21305)
                     /gene="exuR"
                     /locus_tag="KW011_001244"
     CDS             complement(20529..21305)
                     /gene="exuR"
                     /locus_tag="KW011_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HBH7659634.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            21601..22317
                     /locus_tag="KW011_001245"
     CDS             21601..22317
                     /locus_tag="KW011_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBH7659635.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFLGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            22347..22847
                     /locus_tag="KW011_001246"
     CDS             22347..22847
                     /locus_tag="KW011_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBH7659636.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            22939..25623
                     /locus_tag="KW011_001247"
     CDS             22939..25623
                     /locus_tag="KW011_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HBH7659637.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            25620..26708
                     /locus_tag="KW011_001248"
     CDS             25620..26708
                     /locus_tag="KW011_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HBH7659638.1"
                     /translation="MRNRLIAAILGLFGTLIGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(26791..28088)
                     /gene="exuT"
                     /locus_tag="KW011_001249"
                     /pseudo
     CDS             complement(26791..28088)
                     /gene="exuT"
                     /locus_tag="KW011_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            28571..29983
                     /gene="uxaC"
                     /locus_tag="KW011_001250"
     CDS             28571..29983
                     /gene="uxaC"
                     /locus_tag="KW011_001250"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBH7659639.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            29998..31485
                     /gene="uxaA"
                     /locus_tag="KW011_001251"
     CDS             29998..31485
                     /gene="uxaA"
                     /locus_tag="KW011_001251"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBH7659640.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            31568..32119
                     /locus_tag="KW011_001252"
     CDS             31568..32119
                     /locus_tag="KW011_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HBH7659641.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(32124..33368)
                     /gene="sstT"
                     /locus_tag="KW011_001253"
     CDS             complement(32124..33368)
                     /gene="sstT"
                     /locus_tag="KW011_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBH7659642.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(33984..34949)
                     /gene="alx"
                     /locus_tag="KW011_001254"
     CDS             complement(33984..34949)
                     /gene="alx"
                     /locus_tag="KW011_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HBH7659643.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(35232..36218)
                     /locus_tag="KW011_001255"
     CDS             complement(35232..36218)
                     /locus_tag="KW011_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBH7659644.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(36297..36980)
                     /locus_tag="KW011_001256"
     CDS             complement(36297..36980)
                     /locus_tag="KW011_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HBH7659645.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(37057..37560)
                     /locus_tag="KW011_001257"
     CDS             complement(37057..37560)
                     /locus_tag="KW011_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBH7659646.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            37645..38781
                     /gene="rlmG"
                     /locus_tag="KW011_001258"
     CDS             37645..38781
                     /gene="rlmG"
                     /locus_tag="KW011_001258"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HBH7659647.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            39066..39380
                     /gene="higB"
                     /locus_tag="KW011_001259"
     CDS             39066..39380
                     /gene="higB"
                     /locus_tag="KW011_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HBH7659648.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            39377..39793
                     /gene="higA"
                     /locus_tag="KW011_001260"
     CDS             39377..39793
                     /gene="higA"
                     /locus_tag="KW011_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HBH7659649.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(39838..41856)
                     /gene="fadH"
                     /locus_tag="KW011_001261"
     CDS             complement(39838..41856)
                     /gene="fadH"
                     /locus_tag="KW011_001261"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HBH7659650.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLNVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(42082..44433)
                     /gene="ygjK"
                     /locus_tag="KW011_001262"
     CDS             complement(42082..44433)
                     /gene="ygjK"
                     /locus_tag="KW011_001262"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBH7659651.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(44450..45527)
                     /gene="ygjJ"
                     /locus_tag="KW011_001263"
                     /pseudo
     CDS             complement(44450..45527)
                     /gene="ygjJ"
                     /locus_tag="KW011_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(45661..45975)
                     /locus_tag="KW011_001264"
     CDS             complement(45661..45975)
                     /locus_tag="KW011_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBH7659652.1"
                     /translation="MLPPLFIMLAYLNLRAKLDHLPRDFRMGSRRTGIIVVSMLIAIF
                     AVGFVASTFPTGANILTIIFYNVGGIVIFLGFAWWKYSKYIKGLTAEERHIEATPASN
                     VD"
BASE COUNT        11508 a        11260 c        11787 g        11428 t
ORIGIN      
        1 caaactttct ttttaccgtc caataatcaa tatttctatt gtgattgtta accactgcgt
       61 gcattggcgg gattgaaggg tgaaaaatat gtgcaattgg gaagcctttc attgatgcct
      121 gccttgtcgt catgacaaac aataatagat gcttattttc ctcgcagggt tctttcgcac
      181 tatcaaaaaa cgctcattta cccggcgtta cagtaaacga agcaacattc tgtggcctac
      241 gacgatcaaa agctctgtga aaatttccct tgttgtcttt gtctgacctt atcgcggcga
      301 tcagcaatta ttcgttttgt gatcctcaac acaaaatgct attcgtcgcc tatgagggga
      361 gaagaattta tggaaaacga ttgcattcac aaaatgacgt cctgtcagaa gagaaaaagt
      421 tgacgttaat caattgttcg agatgatcaa tacctgtatt cgaaatttta aaatattgat
      481 aaaataaatg tttgttctcc aacaaatagc gtgaaaaaat gtgacgtgcc gcagtattta
      541 tcgctgaaaa tggtttattt ataataaaat ttgagaactt gctcattaaa tttaactcaa
      601 attttgcctg gtaattatcc ggtaattgct tgaaatatgg tctcagcccc ttttttgtat
      661 taaccacata acgaatgatg ttatcgccat aaaatatggt tatccccgtc attttttttg
      721 acaaaaatca gggtttatgc tgatttttat gctttaactt gttgatattt aaaggtattt
      781 aattgtaatg acgataccct ggaaagtatt gaaagttaat ttgtgagtgg tcgcacatat
      841 cctgttcatt tcattttgat acacttcatg ccgtcaatga ggtaattaac gtaggtcgtt
      901 atgagcacta ttcttcttcc gaaaacgcag cacctggtag tctttcagga agtcattaga
      961 agtggctcta tcggctcggc tgcaaaagaa ttagggttaa ctcaaccggc cgtcagtaaa
     1021 atcattaacg atattgaaga ttattttggt gtggaattag tggtgcggaa aaataccggt
     1081 gtaacattaa cacctgccgg tcaattgtta ctctcccgtt ccgaatccat tacccgtgaa
     1141 atgaaaaata tggttaatga gataagcggt atgtcttctg aggcggtggt ggaagtctca
     1201 tttggttttc cttcattgat tggttttact tttatgtcag ggatgatcaa caagttcaaa
     1261 gaggtgttcc cgaaagcgca ggtttctatg tatgaagcgc aactgtcttc gttcttaccg
     1321 gcaatccgcg acggtcgact ggattttgcg attggtacgt taagtgcaga aatgaagctt
     1381 caggatttac atgttgagcc gctgttcgag tccgagtttg tgctggtagc cagtaagtcc
     1441 cgaacatgca ccggcaccac cacgctggag tcgttgaaga acgaacagtg ggtgttgcca
     1501 caaactaata tggggtacta cagcgaactg cttactacgt tacaaagaaa tggcatcagt
     1561 attgaaaaca tcgttaaaac cgactcagtc gtgacaattt ataatcttgt tctcaatgct
     1621 gatttcttaa ctgtaattcc ttgtgatatg acgtcacctt ttggttctaa tcaatttatt
     1681 actattccgg ttgaagaaac attacctgtg gcacaatatg ccgcggtatg gtcgaaaaat
     1741 tatcgtatta aaaaagcagc atcggttttg gtggaattag ccaaagagta ttcatcttat
     1801 aatgggtgta gacgaaggca attaatagaa gttggttagt tatttgtttt tatttaaaca
     1861 taaataatcc acctgtctgt tttgcaggtg tcggttacgg ttacctacat atttaattca
     1921 ggcgaagagg ttttataatg catattacat acgatctgcc gattgctatt gatgatatta
     1981 ttgaagcgaa acaacgactg gctgggcgaa tttataaaac aggcatgcct cgctccaact
     2041 attttagtga acgttgcaaa ggtgaaatat tcctgaagtt tgaaaatatg cagcgtacgg
     2101 gttcatttaa aattcgtggc gcatttaata aattaagttc actgaccgat gcggaaaaac
     2161 gcaaaggcgt ggtggcctgt tctgcgggca accatgcgca aggggtttcg ctctcctgcg
     2221 cgatgctggg tatcgacggt aaagtggtga tgccaaaagg tgcgccaaaa tccaaagtag
     2281 cggcaacgtg cgactactcc gcagaagtcg ttctgcatgg tgataacttc aacgacacta
     2341 tcgctaaagt gagcgaaatt gtcgaaatgg aaggccgtat ttttatccca ccttacgatg
     2401 atccgaaagt gattgctggc cagggaacga ttggtctgga aattatggaa gatctctatg
     2461 atgtcgataa cgtgattgtg ccaattggtg gtggcggttt aattgctggt attgcggtgg
     2521 caattaaatc tattaacccg accattcgtg ttattggcgt acagtctgaa aacgttcacg
     2581 gcatggcggc ttctttccac tccggagaaa taaccacgca ccgaactacc ggcaccctgg
     2641 cggatggttg tgatgtctcc cgcccgggta atttaactta cgaaatcgtt cgtgaattag
     2701 tcgatgacat cgtgctggtc agcgaagacg aaatcagaaa cagtatgatt gccttaattc
     2761 agcgcaataa agtcgtcacc gaaggcgcag gcgctctggc atgtgctgca ttattaagcg
     2821 gtaaattaga ccaatatatt caaaacagaa aaaccgtcag tattatttcc ggcggcaata
     2881 tcgatctttc tcgcgtctct caaatcaccg gtttcgttga cgcttaatta attcgttgag
     2941 gataggatat gagtacttca gatagcattg tatccagcca gacaaaacaa tcgtcctggc
     3001 gtaaatcaga taccacatgg acgttaggct tgtttggtac ggcaatcggc gccggggtgc
     3061 tgttcttccc tatccgcgca ggttttggcg gactgatccc gattcttctg atgttggtat
     3121 tggcataccc catcgcattt tattgccacc gggcgctggc gcgtctatgt ctttctggct
     3181 ctaacccttc cggcaacatt acggaaacgg tggaagagca ttttggtaaa actggcggcg
     3241 tggttatcac gttcctgtac ttcttcgcga tttgcccact gctgtggatt tatggcgtta
     3301 ctattaccaa tacctttatg acgttctggg aaaaccagct cggctttgca ccgctgaatc
     3361 gcggctttgt ggcgctgttc ctgctgctgc tgatggcttt cgtcatctgg tttggtaagg
     3421 atctgatggt taaagtgatg agctacctgg tatggccgtt tatcgccagc ctggtgctga
     3481 tttctctgtc gctgatccct tactggaact ctgcagttat cgaccaggtt gacctcggtt
     3541 cgctgtcgtt aaccggtcat gacggtatcc tgatcaccgt ctggctgggg atttccatca
     3601 tggttttctc ctttaatttc tcgccaatcg tctcttcctt cgtggtttct aaacgtgaag
     3661 agtatgagaa agacttcggt cgcgacttca ccgaacgtaa atgttcccaa atcatttctc
     3721 gtgccagcat gctgatggtt gcagtggtga tgttctttgc ctttagctgc ctatttacgc
     3781 tgtctccggc caacatggcg gaagccaaag cgcagaatat tccagtgctt tcttatctgg
     3841 ctaaccactt tgcgtccatg accggtacca aaacaacgtt cgcgattaca ctggaatatg
     3901 cggcttccat catcgcactc gtggctatct tcaaatcttt cttcggtcac tatctgggaa
     3961 cgctggaagg tctgaatggc ctggtcctga agtttggtta taaaggcgac aaaactaaag
     4021 tgtcgctggg taaactgaac actatcagca tgatcttcat catgggctcc acctgggttg
     4081 ttgcctacgc caacccgaac atcctcgacc tgattgaagc catgggcgca ccgattatcg
     4141 catccctgct gtgcctgttg ccgatgtatg ccatccgtaa agcgccgtct ctggcgaaat
     4201 accgtggtcg tctggataac gtgtttgtta ccgtgattgg tctgctgacc atcctgaaca
     4261 tcgtatacaa actgttttaa tccgtaactc aggatgagaa aagagatgaa tgaatttccg
     4321 gttgttttgg ttattaactg tggttcgtct tcgattaagt tttccgtgct cgatgccagc
     4381 gactgtgaag tattaatgtc aggtattgcc gacggtatta actcggaaaa tgcattctta
     4441 tccgtaaatg ggggagagcc agcaccgctg gctcaccaca gctacgaagg tgcattgaag
     4501 gcaattgcat ttgaactgga aaaacggaat ttaaatgaca gtgtggcctt aattggccac
     4561 cgcatcgctc acggcggcag tatttttacc gagtccgcca ttattaccga tgaagtcatt
     4621 gataatatcc gtcgcgtttc tccactggca cccctgcata attacgccaa tttaagtggt
     4681 attgaatcgg cgcagcaatt atttccgggc gtaactcagg tggcggtatt tgataccagt
     4741 ttccaccaga cgatggctcc ggaagcttat ttatacggcc tgccgtggaa atattatgaa
     4801 gagttaggtg tacgccgtta tggtttccac ggcacgtcgc accgctatgt ttcccagcgc
     4861 gcacattctc tgctgaatct ggcggaagat gactccggcc tggttgtggc gcatcttggc
     4921 aatggcgcgt caatctgcgc ggttcgcaac ggtcagagtg ttgatacttc aatgggaatg
     4981 acgccgctgg aaggcttgat gatgggaacc cgcagtggcg atgtcgactt tggtgcgatg
     5041 tcctgggtcg ccagccaaac caaccagagc ctgggtgacc tggaacgcgt agtgaataaa
     5101 gagtcgggat tattaggtat ttccggtctt tcttcggatt tacgtgttct ggaaaaagcc
     5161 tggcatgaag gtcacgaacg cgcgcaactg gcaattaaaa cctttgttca ccgaattgcc
     5221 cgtcatattg ccggacacgc agcttcatta cgtcgcctgg atggaattat attcaccggc
     5281 ggaataggag agaattcaag tttaattcgt cgtctggtca tggaacattt ggctgtatta
     5341 ggcgtagtga ttgatacaga aatgaataat cgctctaact cttttggaga gcgaattgtt
     5401 tccagtgaaa atgcgcgtgt catttgtgcc gttattccga ctaacgaaga aaaaatgatt
     5461 gctttggatg ccattcattt aggcaaagtt aacgcgcccg cagaatttgc ataatttagt
     5521 tgaagtattg tagagagatt atttttcatg aagatagata ttgataccag cgataagctg
     5581 tacgccgacg catggcttgg ctttaaaggt acggactgga aaaacgaaat taatgtccgc
     5641 gattttattc aacataacta tacaccgtat gaaggcgatg aatctttcct cgccgaagcg
     5701 acgcctgcca ccacggaatt gtgggaaaaa gtaatggaag gcatccgtat cgaaaacgca
     5761 acccacgcgc cggttgattt cgataccaat attgccacca caattaccgc tcatgatgcg
     5821 ggatatatta accagccgct ggaaaaaatt gttggtctgc aaacggatgc gccattaaag
     5881 cgcgcgctgc atccgttcgg tggcattaat atgattaaaa gttcattcca cgcctatggc
     5941 cgtgaaatgg acagtgaatt tgaatatctg tttaccgatc tgcgtaaaac ccataaccag
     6001 ggcgtatttg atgtttactc accggatatg ctgcgctgcc gtaaatctgg cgtgctgacc
     6061 ggtttaccag atggctatgg ccgtgggcgc attatcggtg actatcgccg cgtagcgctg
     6121 tatggcatca gttatctggt gcgtgaacgc gaactgcaat ttgccgatct ccagtcccgt
     6181 ctggaaaaag gcgaagatct ggaagccacc atccgtctgc gtgaggagct ggcagagcat
     6241 cgtcatgcgc tgttgcagat tcaggaaatg gcggcgaaat atggctttga tatctctcgc
     6301 ccggcgcaga atgcgcagga agcggtgcag tggctctact tcgcttatct ggcggcagtg
     6361 aaatcacaga acggcggcgc gatgtcgctg gggcgcacgg cgtcgttcct cgatatctac
     6421 attgagcgcg actttaaagc aggcgtactc aatgagcagc aggcacagga actgatcgat
     6481 cacttcatta tgaagatccg tatggtgcgc ttcttgcgta cgccggaatt tgattcgctg
     6541 ttctccggcg acccaatctg ggcgacggaa gtgatcggcg ggatggggct ggacggtcgt
     6601 acgctggtga ccaaaaactc cttccgctat ttgcacaccc tgcacactat ggggccggca
     6661 ccggaaccta acctgaccat tctttggtcg gaagaattac cgattgcctt caaaaaatat
     6721 gccgcgcagg tgtcgatcgt cacctcttcc ttgcagtatg aaaacgacga tctgatgcgt
     6781 actgacttca acagcgacga ttacgcaatt gcttgttgcg tcagcccaat ggtgattggt
     6841 aagcaaatgc agttctttgg tgcacgcgct aacctggcga aaacactgct gtacgcaatt
     6901 aacggcgggg tggacgagaa gctgaagatt caggtcgggc cgaaaacagc accgctgatg
     6961 gacgacgtgc tggattacga caaagtgatg gacagcctcg atcacttcat ggactggctg
     7021 gcggtgcagt acatcagcgc gctgaatatc attcactaca tgcacgacaa gtacagctac
     7081 gaagcttcgc tgatggcgct gcacgatcgt gatgtctatc gcactatggc atgcggcatc
     7141 gcgggcctgt cggtggcgac ggactccctg tctgccatca aatatgcccg cgtgaaacca
     7201 atccgtgacg aaaacggcct ggcggtggac tttgaaatcg acggtgaata tccgcagtac
     7261 ggcaacaacg acgagcgcgt agacagcatt gcctgcgacc tggttgaacg ctttatgaag
     7321 aaaattaaag cgctgccaac ctatcgcaac gccgtcccta cccagtcgat tctgactatc
     7381 acttctaacg tggtgtacgg ccagaaaacc ggtaatacgc cggacggtcg tcgcgccgga
     7441 acaccgttcg cgccgggcgc taacccgatg catggtcgtg accgcaaagg tgccgtggcc
     7501 tcattgacgt cggtggcgaa actgccgttc acctacgcca aagatgggat ctcgtacacc
     7561 ttctcaatcg ttcctgcggc gctgggcaaa gaagatccag tacgtaaaac caaccttgtc
     7621 ggcctgctgg atgggtattt ccaccacgaa gcggatgtcg aaggcggtca acacctcaac
     7681 gtcaacgtaa tgaatcggga aatgctgctg gatgccatcg agcacccgga aaaatatcct
     7741 aacctgacaa tccgtgtctc tggctacgcc gtgcgcttca acgcactgac ccgtgaacag
     7801 caacaggatg ttatttcacg tacttttacc caggcgctct gacgccggag gatgtatgaa
     7861 aaagattatc gaaacgcaac gtgccccagg cgcaatcggc ccttatgttc agggcgttga
     7921 tttaggcagc atggtcttca cctccgggca aattccggtt tgcccgcaga ccggtgagat
     7981 cccggctgat gtgcaagatc aggcgcgttt aagcctcgaa aacgtcaaag cgatcgtggt
     8041 tgctgccggg ctgagcgtgg gcgatatcat caagatgacc gtgttcatca ccgatctgaa
     8101 tgattttgcc accatcaatg aagtctataa gcagttcttc gatgagcatc aggcgaccta
     8161 cccgacccgg agctgtgtgc aggttgcgcg tttgccgaaa gatgtgaagc tggaaattga
     8221 agctatcgca gtacgtagtg cgtaaagcct cgtgagcggg acggtcgcaa ggtcgttccg
     8281 ctccacttca ctgaacggca atccgagggt gtggatatga ttagtgcatt cgatattttc
     8341 aaaattggga ttggtccctc cagttcgcat accgtggggc caatgaatgc cggaaaaagt
     8401 tttattgatc ggctggaaag tagcggctta ttaaccgcga cgagccatat tgtggtcgat
     8461 ctgtatgggt cgttgtcact gacgggcaaa ggccatgcca cggatgtcgc cattatcatg
     8521 ggactggcag gaaacagtcc gcaggatgtt gtcattgatg agatccctgc atttatagag
     8581 ttagtaacgc gcagcgggcg gctgccagtg gcatctggtg cgcatattgt tgattttcct
     8641 gtagcaaaga acattatctt ccatcccgaa atgttgcctc gccatgagaa cggaatgcgg
     8701 atcactgcct ggaagggaca ggaagcgcta ttaagtaaaa cctattactc tgtcggcggc
     8761 gggtttattg tcgaagaaga acacttcggc ctgtcgcacg atgtcgaaac gtccgtacct
     8821 tacgatttcc actcagcagg tgaactgctg aaaatgtgtg attacaacgg cctgtctata
     8881 tctggcctga tgatgcacaa cgagctggcg ctgcgcagca aagcggaaat tgacgcagga
     8941 tttgcccgta tctggcaagt gatgcatgac ggtattgaac gcgggatgaa cactgaaggc
     9001 gtgctgcctg gtccgctcaa tgtgccgcgt cgtgccgtag cgctgcgtcg tcagctggtt
     9061 tctagcgata acatctctaa cgatccgatg aatgtcatcg actggatcaa catgtacgcg
     9121 ctggcggtta gtgaagaaaa cgcagctggc gggcgcgtgg taacggcacc gactaacggt
     9181 gcgtgcggca ttattccggc agtgctggct tattacgata agttccgtcg tccggtaaac
     9241 gagcggtcaa ttgcccgcta ttttctggca gcgggggcta ttggcgcgct gtataaaatg
     9301 aacgcctcca tctctggcgc ggaggtcggc tgtcaggggg agattggcgt ggcctgttca
     9361 atggcggcgg cagggttaac tgaactactg ggcggcagtc cggcgcaggt atgcaatgcg
     9421 gcggaaatcg cgatggagca taaccttggg ctgacctgcg atccggttgc cggacaggta
     9481 caaatcccgt gcattgaacg taatgccatt aatgccgtga aagcagtaaa cgccgcgtgg
     9541 atggcgatgc gccgcacctc ggcaccgcgt gtttcactcg ataaagtgat cgagacgatg
     9601 tatgaaaccg gcaaagatat gaacgataaa taccgcgaaa catcacgcgg aggactggcc
     9661 attaaagtgg tctgcggctg acattgctgt cagctttcat ccttgggtgc acaaatgtgc
     9721 accttttttt tgtgatctgc cgcaaataga gaatgaatat tctcttgcga ttaatttagt
     9781 agaaaattct tatccatctc cgttatttca aagtcttgat tgtgaagatt ttctctgacc
     9841 tcgcgcgtat catttcttta ttctaataac tcgcctgcaa aaacgtagac cgcatatcgc
     9901 cttgatgcgg ctcggaaaaa aataataagc cctacaaaat attgagcgag aaattatgga
     9961 aattgcatcg aataaaggcg tcattgcaga cgcttcgacc ccggcgggtc gtgctggaat
    10021 gagtgagagc gagtggcgag aagcgatcaa attcgacagt actgacaccg gctgggtgat
    10081 tatgagtatc gggatggcga ttggcgcggg gattgttttt ctcccggtgc aggtcggttt
    10141 gatgggattg tgggtatttt tgctctcatc ggtgattggt tacccggcaa tgtatctgtt
    10201 tcagcggttg tttattaata cgctggcaga atcaccagaa tgtaaagatt acccgagtgt
    10261 cattagcggt tatttaggta aaaactgggg catcctgtta ggtgcgttct atttcgtaat
    10321 gctggtgatt tggatgttcg tctattccac cgccatcacc aacgatagtg cttcctatct
    10381 gcataccttc ggcgtgacgg aagggttgct gtcagacagt cccttttatg gtctggtact
    10441 gatttgcatt ctggtggcga tctcttcacg cggcgagaaa ttgttattca aaatttcgac
    10501 cggcatggtg ctgaccaagc tgctggtggt cgcggcgctg ggcgtgtcga tggtgggaat
    10561 gtggcatctg tataacgtcg gttcgctacc gccgctgggg ctgctggtga aaaacgccat
    10621 tattacgctg ccgtttaccc tgacgtcgat tttgtttatc cagacgttaa gcccgatggt
    10681 gatctcttat cgctcgcggg aaaaatctat tgaagtggcg cggcataaag cattgcgggc
    10741 aatgaatatc gcgtttggca ttttgtttgt caccgtcttt ttctacgccg tgtcgttcac
    10801 gctggcgatg gggcatgacg aagcggtaaa agcctacgag cagaatattt ccgcgctggc
    10861 gattgccgca cagtttatta gcggtgacgg cgcagcgtgg gtgaaagtgg tcagcgtcat
    10921 tctcaatatc tttgctgtca tgactgcatt ctttggcgtc tatttaggct ttcgcgaagc
    10981 aacgcaaggg atcgtaatga acatcctgcg tcgcaagatg cctgccgaga agattaacga
    11041 aaatcttgtt cagcgcggca tcatgatttt cgccattttg ctggcctgga gcgctatcgt
    11101 actgaacgca ccggtgttga gcttcacctc tatctgtagc ccgattttcg gcatggtagg
    11161 gtgcctgatc ccggcgtggc tggtttacaa agtaccggca ttgcacaaat acaaagggat
    11221 gtctctgtac ctgattatcg tcactggttt gttgctttgt gtttctccgt tcctggcatt
    11281 ttcttgattt acctgaaatt ttaaggtttt taatatgttt gattcgactt taaatccgtt
    11341 atggcagcgt tacatcctcg ccgttcagga ggaagtaaaa ccggcgctgg gatgtactga
    11401 accgatttca ctggcgctgg cggcggttgc tgcggcagaa ctggaaggtc cggttgaacg
    11461 tgtagaagcc tgggtttcgc caaatctgat gaagaacggt ctgggcgtca ccgttcccgg
    11521 cacgggaatg gtggggctgc cgattgcggc ggcgctgggg gcgttaggtg gaaatgccaa
    11581 cgccgggctg gaagtgctga aagacgcaac tgcgcaggca attgccgatg ccaaagcact
    11641 gctggcggcg gggaaagtct ccgttaagat ccaggaacct tgcaatgaaa tcctcttctc
    11701 acgcgccaaa gtctggaacg gtgagaagtg ggcgtgtgtc accatcgtcg gcgggcatac
    11761 caacattgtg catattgaga cgcacaatgg tgtggtgttt acccagcagg cgtgtgtggc
    11821 agagggcgag caagagtctc cgctgacggt gctttccaga acgacgctgg ctgagatcct
    11881 gaagttcgtc aatgaagtcc cgtttgcggc gatccgcttt attctcgatt ccgcgaagct
    11941 aaattgtgcg ttatcgcagg aaggtttgag cggtaagtgg gggctgcata ttggcgcgac
    12001 gctggaaaaa cagtgcgagc gcggtttgct ggcgaaagat ctctcttcat ccattgtgat
    12061 tcgtaccagc gcggcatccg atgcgcgtat gggcggcgct acgcttccgg ctatgagtaa
    12121 ctccggctcg ggtaaccagg ggatcaccgc aacaatgcct gtggtggttg tagcagaaca
    12181 cttcggagcg gatgatgaac gactggcgcg tgcgctgatg ctttctcatt tgagcgcaat
    12241 ttacatccat aaccagttac cgcgtttgtc tgcactttgt gccgcaacga ccgcagcaat
    12301 gggggccgcc gccgggatgg catggctggt ggatgggcgt tatgaaacta tctcgatggc
    12361 gatcagcagt atgatcggcg atgtcagcgg catgatttgc gatggtgcgt cgaacagctg
    12421 cgcgatgaag gtttcgacca gtgcttcggc tgcgtggaaa gcggtgttaa tggcgctgga
    12481 tgataccgcc gtgaccggca atgaagggat cgtggcgcat gatgttgagc agtcgattgc
    12541 caacctgtgt gcgttagcaa gccattcgat gcagcaaacg gatcggcaga ttatcgagat
    12601 tatggcgagc aaggccagat aaacgccagg ttttgagtat tgtgccggat gcgatgctgg
    12661 cgcaccttat ccggcctacg ggttgtttaa ttcgttaatg ttgttggatt ttgtaggccg
    12721 gataaggcgt tttcgccgca tccggcaacc tgtctttacg cggtagcttc atcctcggct
    12781 aaacgcgttt cagcatccgc gacgatggct tccagctcgc tcaacatctc ttcatagcca
    12841 aaatttttgg cggtgcgagc aggttctttc gccaccacgg gcttcactgg ctggcgcttt
    12901 ttggccgatt ttttactcat catcatctcc ctgtaaaaac aactagacag gcaaatctat
    12961 cagcaaagcg cgcagtgggg aatcggcaac cagcgtaatg ttagcctcgt cacgaataaa
    13021 cgccccatca ccgcaggtca gcgccgcttt ctcttcatga tgcgtaagcg catgaaattt
    13081 cccgtgaatc gattgcaaat acgcgcgtgg cccatgcaac tggaaattcg cactttcgcc
    13141 tttgtcgagc acgatatggt gcagccacac ttgctggcgt aattgcaggc ttcccatcgt
    13201 accctctggc gaggcgatta attgctgctt gcccatatta agcgccagtt tttgaatcag
    13261 cggattctct cgctgcgggc aggcgtccag ccaaagctgc attcgtgtta acggtttgtc
    13321 tttgctgaga ttgtgttcgc tgtagctgac acccggctgg gtagagagca gcaacgcctc
    13381 accggcgctg gcctgaacat gattgccttc gctgtcgcga tactctgctt ctccatccag
    13441 aatcacattt aaaatatcga ctttgggata ggttcgcggc tgaaaggcgg cacctggggc
    13501 cagcacttcc tggttaagca cacgcaggga ggcatagcct aacaatttcg ggtcgaagta
    13561 gtgtccaaag gaaaaagtat accgggcctg caaccatccg tagtctgctt gtccacactg
    13621 cctggcagtt cgggtagtaa tcataattct tgacctctct ttacttcctt ttatggtaag
    13681 gggctggacg ctacattgtt agccagatat tctgcccggt atgttcaaat ttcctgaatg
    13741 agaacgaaat ggccaaagaa agggcattaa cgctggaagc actacgggtt atggatgcga
    13801 tcgccggggc agttttgcgg cggcggcgga tgagctggga cgcgtgcctt ccgcacttag
    13861 ctacaccatg caaaaactgg aagaagagct ggatgtggtg ctgtttgacc gctcgggcca
    13921 tcgcaccaaa ttcaccaatg tcgggaggat gttgctggag cgagggcgcg ttttgctgga
    13981 agccgcagat aaactgacta ccgatgcgga agccctcgcg cgcggttggg aaacgcatct
    14041 caccattgtg accgaagcgc tggtaccgac acctgccttt ttcccgttaa tcgacaaact
    14101 ggcggcaaaa gccaataccc aactggcaat catcaccgaa gtgctggcgg gggcgtagga
    14161 acgactggag caggggcggg cggatattgt tatcgcgccg gatatgcatt ttcgttcctc
    14221 gtcggagatc aactcgcgca agctctatac gttaatgaac gtctacgttg ccgcgcctga
    14281 tcacccgatt catcaggagc cggaaccgtt atctgaagtg acgcgcgtga aatatcgtgg
    14341 aattgcggta gcggataccg ctcgtgagcg cccggtgttg accgtacaac tgctggacaa
    14401 acagccgcgc ttaacggtga gcacgattga agataaacgt caggcattac tggcggggct
    14461 tggcgtggcg acgatgccgt atccgatggt cgaaaaagat attgcggaag ggcggttgcg
    14521 tgtcgtcagc ccggaatcga ccagtgagat cgatattatt atggcctggc gtcgtgacag
    14581 tatgggggaa gcgaaatcct ggtgtctgcg ggaaattccc aaacttttta gcggaaaata
    14641 atatttatta ttgttaataa aaaggggcga aatatcgccc caattctaag attaatttga
    14701 accaaacttc ggatcgtttc cgtaacgatt gctgccagaa gtaccttctg tgcagaaaaa
    14761 aaccagaagc acgagccagc caataaacgg aacaaaaaag agcaaggccc aggcacctga
    14821 acggtcggta tcgtgtaagc gacggatagc aagcgcaagg acgggaagga aggttgccag
    14881 caaataaagc atagacagat atggaagctc cagacctaaa atcaattgaa tgacattgat
    14941 aatagcgccg acgatggcgt taatcagagt aaacatccag tactctttac gacgtgcgcg
    15001 accagagaaa ccaacataat tttttaatac ggataaatac cactgcataa aaacatccct
    15061 ttaattaaaa gtgacgaaag tctattccgg gtattgtgta aatgctattc gtatctttct
    15121 ttttcggatg ttataaaggt tttgatattt aagataaata catggttatt ttttataggt
    15181 aatttatata acgacattga aatattttaa ataaacatta gtaaattatt cattcatcaa
    15241 catgatgata aaagccggat gagtcagaca tccggcgagc agatttgttt taaggttcca
    15301 gttttggatc aggtccaaag cggttttcgc ctggcgtacc cgcctggcag ttaaagacga
    15361 tgataataag ccagccgata aacgggatta agaatagcag tgcccaccac gccgagcggt
    15421 cggtgtcgtg caggcggcgg aactgaactg cccaccacgg taaaaacacc agaataccgt
    15481 agattgtcgt caggatgcct tcgccaccag cgcgttgcca gcctaacatt ttatccagca
    15541 gccccagcac gaacgtaaag atgatgttga ccagaataaa catccagtac tctttgcgcc
    15601 gcgcacgacc ccggaaaccg acataatttt ttattacttt cagataccag tccatttttg
    15661 cctcaatttt gctcaatcac ttgtttttaa agcagcaaat taagaataga cgtgaatgaa
    15721 atggcagtaa atattcgttc aaggaattaa taaagtgctg tgaaggcgcg ctaagactga
    15781 tgacaagcgc aaaatcgcca gatgcgctac gcttatctgg cctgcgcggt cattgcaaca
    15841 tattgaatat aaatgttttg taggtttgat aaggcgttca cgccgcatcc gatatgaaca
    15901 acatgcactt agctaattat cagaggcact tcgtaaggtg ccttttttta accgaagcga
    15961 acatctcgtc ctgcggttca tcgagatcct gccacggacc aattgaaata atccccgtag
    16021 ggttgatggt cttatggctg cggaagtaat gattacggat gtgatcgaaa ttgacagttt
    16081 cggcgatccc cggcatctgg tagatatcgc gcaggaagcc atacagattc aggtaatcgc
    16141 tgatgcggtg cttatcacac ttgaagtggg tcacatacac tggatcaaaa cgcaccagcg
    16201 tagtccacag gcgaatatcg gcttcggtta gctggttgcc ggtcaggtaa cgatgctggc
    16261 ctaaaatctg ctccagtcgc gccagcgatt caaacacttt cgccaccgcc tcgtcgtagg
    16321 cttgctggct ggtggcaaaa ccagctttat acacgccgtt gttaacggtg tcataaatcc
    16381 agccgttaag ttcgtcaatt ttcgtttgca gggctggtgg gtagtaatca cccgctttcg
    16441 cgcccagcgc atcaaacgcg gtattgaaca tgcggatgat ttctgttgat tcgttgctga
    16501 cgatggtgtg gttctttttg tcccacagta cgggaacagt aactcgtccg ctgtagtgtg
    16561 gatcggcgtg gagataaagc tgatacagaa attcatgttg atagagcgta tctccggttg
    16621 ctcccggaaa actgtcatca aaggtccagc cgttttccag catcagcggg ttcactacgg
    16681 aaacggaaat aaacggttcc agtcctttga gtttgcgcat gatcagcgtg cggtgcgccc
    16741 acgggcaggc gagtgaaata tagagatgat aacgatcttt ctctgcgata aaaccgcctg
    16801 tgccagtggg gccaggtgcg ccatcggcag tgagccagtt acgaaatgcg gaagctgaac
    16861 gttgaaattt accgccggta gatttggtat cgtaccaggt gtcatgccag acgccgtcaa
    16921 tcagttgacc catttttctc tcctccaata gcaaaaacga ggagcttcct cctcgttttt
    16981 tatcgttaag tatagcgcgt gcttaccact ttttattcag caggcggtcg atgctatacg
    17041 cgcccggacc ggtaattgcc agcagcagga atccgccaga aattgtcagg tttttcatga
    17101 acatcagcga gttgacgcct tcagcaaagt tgctgtgaaa taaaaatgcc gtcagcagcg
    17161 taaagcccgc agtaaacagg gctgtggtgc gagtcaggaa accgaacagg attgccagac
    17221 caccaccgaa ctcaagcaga atcaccagcg gcagcataaa acccgggaca cccattgctt
    17281 ccatatattg ttgggtacca gagtaagcgg tgatttttcc ccagcctgcg gtaataaaca
    17341 gaatcggcat taaaatgcgc gctaccagta caccaacatc ttctaatttt ttcatcatac
    17401 tctccactgc gccactcagg ctgctgattg tttttttgtg caagggcgcg gtattagcgt
    17461 cgttgctgtc gatggagaga atcataaacg tggtgaatga tgattgttag caaggaaaac
    17521 tgtcaaaaat cttcaaaaaa tttgagggat aaggccggaa ttgctccggc cagagggaag
    17581 ttaaccgcga agctgttgct gcttgagggt cgttttaacc agacgccagg cgctccatac
    17641 gccaaaaccg cgtctggccc agcggaccag catattagga tggcgaatcg tccagatcgc
    17701 catcacgcta ctgccaacca gcgcccagga gcgcagactt agcagcatat tccagcgacg
    17761 atcgtaagcg cctgttgcct ccagccattc acgacgactg gcggaaagat ccagccgttg
    17821 ttgctggatc tggctaagca gttgcgcctt acgtcgttca cgttcgactt tactgctcac
    17881 gggactcctc ctcgagcagt tgccgatcgt ttgccagctc atggcgtgta tggcgcagca
    17941 aggtggattt acgcgattta cgtaacgtcc agataccgcc aatcagtgcc agtagcagca
    18001 acaccacggt ggtggcaatc atcgcattca ggcgatattg cggatcaacc gcccaaataa
    18061 ttagcaccat caggctcata agaccaaatg cagcgaaaag catcgtcagg cccagcatca
    18121 gtaaaagttg aaagagatta gctttttcct cttccagctc caccaccgcc agccgcagac
    18181 gtgtttccac catttcaacc atgatggaaa caattcgctg cccgatgccc agaacgcttt
    18241 taccgggccc ttgcgcgtga tgagtgtccg ccataattaa cgacgcgaca gcagaacgcc
    18301 gagcactaca ccgattgcag cgccaattcc cacgccggtc cacggatttt cacgcacata
    18361 ctcatcagca cgcgccgccg cgacacgggt ttgtttggcg atggcatcac cggtttcacc
    18421 caggcgataa cggctctgtt tcagtgcctg ctccgctttg ctacgaatct tactcaactc
    18481 ttctttcgac ttctcgccag atgagctaag cacctcttcc agcgtatcgg aaagggattt
    18541 caactcagca cgcagatgtt ccgtagtgtg ttctttcgac atagttttct ccaggtgagt
    18601 aagtagtgac tattgtgagt tagtagtcgc gcgcttccag ctttttcagc tcttcctgcg
    18661 cttctgccag tttccgttcg cgtttggcaa tcttatcggc atcgcctttt tgcttcgcct
    18721 cggctaaatc ttgctggcgt tccgccacct catctttctg ctttgcgatt ttcttgtgat
    18781 gatcggcacg cagctggcta tctgaacagt tggcccggac ttcactcagg gctttattca
    18841 gaccgtcaat acgattctgg ttttggtgtt tttcggcata gctgatctcc ttaaggatat
    18901 tttgctcctt ttcctgacac agggtagtgg cataactacc ggcactaaga gcaaagagag
    18961 aaacagctaa agcgatgcgg tatttcattc ttgaaccttc cattgtgatt cggacctatt
    19021 acaaaggaac gattgtccag acaacgaagc ggtagtgtta accaccgcct cattaatcat
    19081 agacagttac ggtgaaatca ccaaagtgag tgatatgttt cggataacag gaagttatcc
    19141 gaagcgatga gagttatccc gtaaccgggt cagccactgc atagcctgac tgttattgtc
    19201 ctgctgcgca atgatgtagc catggggcaa tgttctgtcg aggaccgctt ttgcggcggc
    19261 gctctggtcg ctggagtcaa atgtaattaa cagagtgtcg tttttggggg tgatactttt
    19321 gaaaggaatg ccatgagcgt caaggtgatg ccagatcgaa aaaccgtctg gcatcgttgt
    19381 gccttgatga acggcacgaa tcgccagcgt agactcttgc tggcgaaccg cggaccaggc
    19441 cagcaacagc gtgccgacca gaagtaaaac agtgccggac caggctagct ggcgaagcga
    19501 catgcgaggt atttgcatcc cttacccccg atttccatat ttctttttcc ataacacgac
    19561 cagagaacct gccaggccaa acaccagcag caccaccggg agcagcatca ggcatgacat
    19621 cagctggtcc tcgtacttta aaaatatcgg cgttttgccg agcatgtaac ccagagttgt
    19681 caggatcaat acccacagca gaccgctcat ccagttgaaa aactgaaagc gcgcgttatt
    19741 cagccctgat aacccggcaa tcgtcggcag cagtgttctg acaaacgcaa taaagcgacc
    19801 aattaacagc gccgataaac cgtgtttatg aaaaagatgg tgtgcgcgtt gatgataatg
    19861 cgcgggtaaa tgagatagcc agttttgtac ggtgcgggta ttgcccagcc atcgcccctg
    19921 aatatagctg acccagcagc cgaggctggc ggcaacggtc agcagcagaa tcgtttgcgg
    19981 gtaacccatc gcgcctttcg caatcaacac gccgaccaat accagtaaac tgtcgcccgg
    20041 taaaaaggcc gctggaagca agccgttttc aaggaacaaa attacaaaca agacaaaata
    20101 caacatgcca atcatcgatg gattggccag ggtttcaaaa tcctgcgccc acagggcttg
    20161 cagcaattgg gtcaaaagtt ccattcatta ttcctggtga atcggttaac gccacgctgg
    20221 ttttaaaaga tgcactaccg caatgttatt gctgttttgg tatgttctgc gggtttgata
    20281 catcaacaca gcctgaacta ttccctgtta aatcgtgaga taagaaagca ttcaaaaaca
    20341 taaattcaaa ttgaatctaa ttgtaacaaa ggtcaacgtt gttcgttatc aaaattacat
    20401 gtccgtggag tctgattttg ctgaatgcag gggagggtgg cgagaggatt tacacaggct
    20461 gacactttat acttttcgct taccgacgaa gcgaacaggc gcgtcgcagc gcctgtcgga
    20521 caggagtttc atttactgcc actggtggcc gtatcgagat gcactaccgg gttttcggcg
    20581 aatagatagc ggtcggcatt gaactcgaaa tcgtcgctgg tttcgttaaa taacatgatt
    20641 ttggtgtttt ccagatgctg ccacattgcc agcttagcag catggggatc tttgcgaatt
    20701 aacgccttga gaatttgatc gtggtcatca caccagttat caacggtacg ggaatcaatg
    20761 tgttcgtgta gttttttcca gtacgggtta tggctacgct gggtccacat tttttcaacg
    20821 atggccgcca gggcggagtt ctgcgtcgcc agggctacct gaatgtggaa ctgtaaatcc
    20881 cactcggaat cacggaagca ttgttcgccg cgcgcctgtt cctgaatggc catcagtttc
    20941 atgatgtctt gtttcgttac ctgagttgcc gcgaactcgg caatattact ttcgatgagc
    21001 tggcgagcct gaagcaactc aaacggaccg taattggcga attccatatt attgtcagcc
    21061 gcctgctgat ggcgcggctg gttggaaacc acatgaatac ctgaaccttt acgcacctcc
    21121 acatagcctt caacttccag catgatgatg gcttcacgaa ctaccgttcg gctgacgttc
    21181 ttttcatcgg caataaagcg ttcggcaggc agtttatcac ccaccagata gacgccctgt
    21241 tcaatgcgct ctttcaggtc agcggcaagt tgttgataca aacggcgtgg ttcagtgatt
    21301 tccatatgcg tctcccggag tggaatgcgg acgaagttat cataccactt tattcatggg
    21361 tatgccattg gtcatatata aatttggtga ttgaattaat gtattgaatt aatattgtga
    21421 atatttataa attcctaaaa aataagtatc gtcattgtga aagctactaa atttcagtaa
    21481 gttaattccc agcctttaaa tttaatatta actaaaaatg cttttttata ttgacttaca
    21541 ataaatctac atctctaata caccaacttt ttggaaacaa tattgtaaga tcaaatttat
    21601 atggtggcga aaattaatct cttatcacgg cttacgccgc ttttattcgt gttcgctcct
    21661 tttttggcac agtcaaatat gactgtttat cctatggctg tttccattaa tagtcagggt
    21721 gaaggcaacg ttcgcgtaat atctaaaagt aatgaagttc agtacatcaa ggcgacagta
    21781 ttccgtatcg ataatccatc gacgcctcag gaaaatgaag ttgagattaa atccggtgat
    21841 gcaaatcatc tggttgttat gccacctaaa ttcgctttac cagccggtag tagcaaaact
    21901 gtgcgctttg ttgcaatgga accagagcaa aaagaaaaaa attatcgcgt taaatttgaa
    21961 gcggttccca gtattgatga cgttgccaca gataaaaaag atctctctat gcagttaaca
    22021 gttaacttaa tttgggggat tgttgttagt gttccacctc agcaacctat tgctaagtta
    22081 gaagtaaatg ctgcccaaaa attagttaat gcagggaatc aacgcttaaa gattttaacg
    22141 atcgcttatt gtaaaaataa tagcaaagaa aattgtaaga tacagaccgt aaataaaaac
    22201 atcttccttg gtcaggaaag aaatcttgaa agcatatctg gctacgacaa aatcgttgtc
    22261 aaatataata actggatcac caaagataat ggcgagtttg aactggcggt ccattaatta
    22321 tcattgtaat aaacgaagga atagtaatga aaaaggtatt tgcaaaatct cttctggtcg
    22381 cagcgatgtt ttctgttgca ggctccgcat tggcagtaca aaaggatatt actgtaacgg
    22441 ccaacgttga tgccgctctg gatatgacac agaccgataa caccgcgttg ccgaaagcag
    22501 tagaaatgca atatctgccg gggcagggtc tgcaatctta ccagttgatg accaaaatct
    22561 ggtctaacga cgttaccaaa gatgtaaaaa tgcagttagt ttccccggcg caactggttc
    22621 aaagcctgga tgctagcaag attgttccgc tgacagtaac ctggggcggt gaagaaatta
    22681 aagctgatgc ggcaaccact tttaccgcaa ccaaaatctt tgcttccgat gcgttaacta
    22741 acggttctct ggctaaaaac ctgatgtttg ctcaaaccac caaaggtgtt ctggagacag
    22801 gcatctaccg tggtgtagta agcatttatc tgtctcagga tatctaatcc agataacaga
    22861 attaacacgt aacaaaggga ggggtagccc tcccttattt taactgtttt agtatttgtt
    22921 atggatttca ggattgccat ggataaaaaa ttactggctc ttttgatcct ggcgagtctc
    22981 agcccggcag aggcgacatt aaccgaaatt cccgcagggt ttgaggttat tgctcaggga
    23041 cagcaggagt atatcgaggt ttatttttca gggaaaagtc tcggtaaata ttatgcaatg
    23101 gttaatcttg ataccgtaac atttcttgat ccagcaagtc tatataacaa gctggaactg
    23161 gatgtagacg atcagaaaat cgcgcatata gtgaaagaaa aattatcgca gccgctagct
    23221 cgccacggtg aattggcttg cggttatgta cgtactgact cagggtgtgg ttttctgaat
    23281 accgatacgc tggaaataat ctataatgat gaagaaagtt cggcaacgtt gtttattaat
    23341 ccgcaatgga attcagcttt cgatgcgaag tcattatatt taaatccaga caaaaatact
    23401 gttaatgctt ttatacatca gcaagacatc aatgttctgg cacaggatga ttaccaatcg
    23461 ttgtctattc agggaaacgg tgcgctggga ataacagaaa atagttatat tggtgcacac
    23521 tggaatttca acggttatga tgcagatgat gtcagtgaca gtaatgctga cgtcagcgat
    23581 ctctattatc gttatgattt tttacgtcgt tattatgtgc aggcgggtcg catggacaac
    23641 cgcacactat ttaatgcaca aggcgggaac tttaccttta actttctgcc actcggtgca
    23701 attgatggga tgcgtatcgg gtcgacactg agctatttaa accaggcgca aagccagcaa
    23761 ggaaccccag taatggttct gctttcgcgc aattctcgtg ttgacgctta tcgtaatgag
    23821 caacttttgg gatcgtttta tctcaatagt ggttcgcaat ttattgatac cagttccttt
    23881 ccgccaggca gctacagcgt ggcattaaaa gtctacgaaa ataaccaact cacccgcacc
    23941 gagcttgtgc cgtttaccaa aaccggcggt ctgactgacg gaaatgcgca atggttctta
    24001 caggcaggta aaactacatc acaggtttct gatgatgaaa gttcagctta tcagctagga
    24061 gtacgcctgc cattacatcc gcaatatgag ctctacgcag ggctggcgaa tgccgatgat
    24121 gtgagtgctt tcgagttagg taataactgg acggcagatt taggcggggt ggggaatctt
    24181 gcaatcagcg ccagcgtgtt ccgtaacgat gacggcggca aaggtgatat gcaacaggcc
    24241 aactggagta atccgggatg gccgacgttg ggcttttatc ggaccaactc tgacggcgat
    24301 gcttgtacaa ccgacagcag agagagctat aacgccttaa gctgttatga aagtatttcc
    24361 gcgacggttt cacagaattt tgtcggctgg aatatgatgc tgggttatac ccgcacacaa
    24421 aataacactg atgatagttt gcgttgggat aaacagcaga gctttgaaaa taactatctt
    24481 cgccagacaa cagcgcaaag tatctctgaa actgtacaac ttagcgcttc ccgcgctttt
    24541 gtgatgcgtg actggatttt gagtacgtca gtcggtgttt tccatcgtaa tgacaacggt
    24601 ggcgataacg acgacaacgg cttgtactta tcgttttcgt tatctgacac gccaacgatg
    24661 gatagcaata acaacagcca ttcaacaaat gtgtctacgg attatcgtta tagcgaacag
    24721 gatggcgatc aaacgtcatg gcagttatcg catacctttt ataacgattc attcagccat
    24781 aaagaacttg gcgtaaccgt cggaggcctg aacaccgata ccataaacag cgcggttaac
    24841 gggcgttggg atgggcaata cggaaatgtc tacgctaccg tatctgatag ttatgaccgt
    24901 aagaatcatg atcatctttc ggcctttacg ggcacttata gctctacgct ggctgtcagt
    24961 tgctatggcg ttaatttggg agccagtggt acagacgatt tgctgggtgc ggtactggtg
    25021 gatgtgaaag gcttctctga acaggatgaa gagagtcagg atctgcaact cgaagcgcgg
    25081 gtggcaggca gccgaacttt gcagcttggt caaagtgaca gtgtgttgtt cccttatcct
    25141 ggatttcagt ctggttttgt tgaggttaac gacagtagcc agggcaatca gcaggggaca
    25201 acaaacatca ttaacggtgc agggaatcgt gaattaatgt tgttgcctgg caagctgcgc
    25261 tatcgcgaag tgtctgccag ctttaactac aactatatcg gtcgtttatt actgccggca
    25321 gcggtgaaaa aattcccgat tgtggggctg aacagcgcca tgttactggt agctgaagat
    25381 ggaggattta cacttgaaat taacggcagc gaaaaagagc tctatctgct ttccgggcag
    25441 caattcctta agtgtccact gagtgttgta aagaaacgcg ccagcattcg ttacagcgga
    25501 gatgttactt gtagtgtggt gacttattca caattaccgg agtcgattca ggttcaggca
    25561 cagttgaaac agcctaaatt acgtggaaac gttcagacgg cgcaaaggga ggttgcacca
    25621 tgagaaatcg attgattgcg gcgatattgg gcttgtttgg cacgctcatt ggcgttcagg
    25681 cagctcctga cgtgaccagt gaaattacgt atgatttggc atctggtaga gcggattatt
    25741 acttctggaa ggatgaagcg tcggcaggaa ataatggata tatgtggtat gaatgttcgt
    25801 atcctgacct ccaacaaacc tgtacagcta atggaaatat atcgacagta caaatctatt
    25861 taactgaaca acgcagtggg atgcgttggc cggtaaaact caaaggattt aaaacagcca
    25921 ttgtaagtag cgatgaagcg ccgccaggat gcaagggggg caaagggctt cagacgaatc
    25981 ttaaggattc taatagatct tcatgtacag aagatggtca acattattat atatacgata
    26041 caaagtttct tacgctctac cttgagcaga cagagatgaa gaatttgccg attggtggcg
    26101 tctggaaggg gaaagttaaa ttacattcga acagcccggc ccaggactat ttcgcaaata
    26161 ttaccctgaa tacgctcgac cccaaccata ttgacgtgtt cttcccggag ttcgcccacg
    26221 ccacgccaag ggtgcagtta gacttgcatc caacaggaag cgttaacggc agcaactacg
    26281 cgcaagatct gaccatgttg gacatgtgcc tgtacgatgg ttttaacggt aatgccatca
    26341 gttatgaaat catgctcaaa gatgaagggc gaccagctgc agggcgcaga gacggtgact
    26401 tctctatcta tcgtcaagga gggaccacca ccgacgaggg agaacgcatt gattaccggg
    26461 tcaaaatgta caacccggaa accggtgggc aaattgatgt gcgcaataat gaaaatatgg
    26521 tctggaacag cattaacctg aaacgtgtgc gtccggtggt actgcccggt attcgctatg
    26581 ccgtgatgtg tgtgccgaca cccttgactc tggcagtcga taaattcagc gtgatggata
    26641 aacaggccgg gtactacatg ggcaaattgt cagtaatctt tacgccttcc ttgccaacca
    26701 tcaattgacc ccgcagacca gaaaaaacta aacaacgaag ccgcctgata aggcggcttc
    26761 gtcattacaa cttacgttaa acgttaaacg ttaatgttgc ggtgcgggat cgttatgtgt
    26821 ttcctgcgcc acctcaatcg ccggtttgtt ctgcaatacg gtccagataa ccagcgcacc
    26881 cagcaggtca aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag
    26941 tgcaccgaca accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc
    27001 gttcgccgtc gccacttcgt tacgaccgaa cacgtcagaa gagagcgtaa tcagcgcgcc
    27061 agacagtgcc tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt
    27121 ggtgaacaga ccgatcatac ccgggccaat catcagcaca gcacccagcg ttacgaccat
    27181 cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc
    27241 accgaggata caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc
    27301 tttcaggtta aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc
    27361 ccaggccggt tctgccagga aacgcggcag tgcgataccc cagaactgac ggttacgcag
    27421 gatctgacca acggacattt tcttcgccgt gctcacctgg tgctgggctt cctgaccatt
    27481 aataatatag tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa
    27541 aatcagccat gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg
    27601 ccagctgtgc atcacgattg ccataccacc agcggcggcg caatcatcgc accaatcgaa
    27661 gaacctacgt taaagtagcc tactgcgatg gaacgctctt tcgccgggaa ccattcggag
    27721 ctggctttca gacccgccgg gatcatcgct gcttccgcgg caccgaccgc accacgagca
    27781 acagccaggc caccccagct acctgccagc gcggttacac cacagaacac ggcccacagc
    27841 acagcaaaca ttgcataacc gattttcgta cccagcacat ccagtacata acctgctacc
    27901 ggttgcatga ccgtataagc agcagaatag gctgcgatga tataggaata ctgttgggtg
    27961 gagatgttta actcttccat cagagttggc gcagctgccg ccacagtgtt acgcgtcagg
    28021 taaccaagca cggtgccgag cgtcaccagt gcgatcatat accaacgtaa ccctttaatt
    28081 ttacgcatgt aaaacctcat cgttttgtta tatccgcagg aagcgcggcc atcatcccgc
    28141 cagaggctga agatgttagt tacgaccggg taaccgaaaa aaaaccggca cgccccgaac
    28201 gttgccatga aagtttcgtg ctaattcggc ttccgtaccg gttaacccga ttcatctaac
    28261 aaatgaatga atcggcaatt tgcattccgt cgacttatga tttgcgacgg cagaaagata
    28321 acttgtcata caactttaaa aggtgagagc catcacaaat gtgcgaatat ttgtagggac
    28381 attacctgac gacagcgagg ccagtactgg cgcggcctgc agcgagattt atcactttga
    28441 gagtaatttt tttaactacg tttattgatc taactcacga aaatatcttc ggactctgga
    28501 aattggtgtg ataactttgt cagcatcgca ccataagcaa gctagctcac tcgttgagag
    28561 gaagacgaaa atgactccgt ttatgactga agatttcctg ttagataccg aatttgcccg
    28621 ccgtctgtat cacgactacg caaaagacca gccgattttc gattaccatt gccatttgcc
    28681 gccgcagcag attgcggaag actatcgttt taaaaacctg tatgacatct ggctgaaagg
    28741 cgatcactac aaatggcgcg ctatgcgtac caacggtgtg gccgagcgtc tgtgtaccgg
    28801 tgatgcatct gaccgtgaaa aatttgacgc ctgggcggcg actgttccgc acactatcgg
    28861 caacccgtta taccactgga cgcaccttga actgcgtcgt ccgtttggta tcactggcaa
    28921 attgctttct ccgtcaactg ccgatgaaat ctggaacgaa tgtaacgaac tgctggcgca
    28981 ggataacttc tccgcgcgcg gcatcatgca gcagatgaac gtgaaaatgg tcggcaccac
    29041 cgatgacccg atcgattctc tggagcatca cgcagagatc gccaaagacg gctctttcac
    29101 catcaaagtg ctgccgagct ggcgtccgga caaagccttc aacatcgaac aggcgacctt
    29161 taacgactac atggcgaagc tgggcgaagt ttccgatacc gacattcgcc gctttgctga
    29221 cctgcaaact gccttgacca aacgtctgga tcacttcgcc gctcacggct gtaaagtgtc
    29281 tgaccatgcg ctggacgtgg tgatgtttgc tgaagcgaac gaagcggaac tggacagcat
    29341 tctggcgcgc cgtctggctg gcgaaaccct gagcgagcac gaagtggcac agttcaaaac
    29401 tgcggtactg gtgttccttg gtgccgaata tgcacgtcgc ggctgggtac agcagtacca
    29461 cattggcgcg ctgcgtaata acaacctgcg tcagttcaaa ctgctggggc cggatgtagg
    29521 ctttgactcc atcaacgacc gtccgatggc ggaagagttg tctaagctgc tgagcaagca
    29581 gaacgaagaa aacctgctgc cgaaaaccat tctctactgc ctgaacccgc gcgataacga
    29641 agtgctgggc accatgatcg gtaacttcca gggcgaaggt atgccgggca aaatgcagtt
    29701 cggttccggc tggtggttta acgatcagaa agacggtatg gaacgtcaga tgacccaact
    29761 ggcgcagctc ggtctgctga gccgctttgt cggtatgctg actgacagcc gtagcttcct
    29821 gtcatacacc cgtcacgaat acttccgccg cattctgtgc cagatgatcg gtcgctgggt
    29881 ggaagcgggc gaagcaccgg cggacatcaa cctgctgggc gagatggtga aaaatatttg
    29941 ctttaacaat gcgcgtgact acttcgccat tgaactgaac taaggtctgg ggttgatatg
    30001 caatacatca agatccatgc gctggataac gtcgcggtcg ctttagcaga tttggctgaa
    30061 ggcacagaag tcagtgtcga taaccagact gttacgctgc gccaggatgt tgctcgtgga
    30121 cataaatttg cgttaacgga tatcgcaaaa ggggccaacg tcatcaaata tggcctgccg
    30181 attggttatg cattggcgga tattgcggcg ggggaacacg tccacgccca caatacgcgc
    30241 acgaatctga gcgatctgga tcagtatcgc tatcaacctg attttcagga tctgcctgcg
    30301 caagcggcag atcgtgaagt gcagatctat cgtcgcgcta acggcgatgt cggggtgcgt
    30361 aacgagctgt ggatcctgcc aaccgtgggc tgtgtcaacg gcatcgcgcg gcaaatccag
    30421 aaccgtttcc tgaaagagac caacaacgcc gaaggtaccg acggcgtgtt cctcttcagc
    30481 cacacctacg gctgctcaca actgggcgac gatcacatca acacccgcac catgctgcaa
    30541 aacatggtgc gccacccgaa cgcgggcgca gtgctggtga ttggtctggg ctgtgaaaac
    30601 aaccaggttg ccgcattccg tgaaacgttg ggcgatatcg atcctgaacg cgttcatttc
    30661 atgatctgcc aacagcagga tgatgagatc gaagccggga tcgagcattt gcatcagctg
    30721 tataacgtga tgcgcaacga taaacgcgag ccaggcaaac tcagcgaact gaagtttggt
    30781 ctggagtgcg gtggttctga cggtctttct ggtattactg ctaacccgat gctggggcgt
    30841 ttctctgact acgtgattgc taacggcggt actaccgtac tgaccgaagt gccggagatg
    30901 tttggcgcag agcagttgct gatggaccat tgccgcgacg aagcaacgtt tgaaaaactg
    30961 gtcaccatgg tcaacgactt taaacagtac tttattgccc atgatcagcc gatctacgaa
    31021 aacccatcac cggggaacaa agcgggcggt atcaccacgc tggaagacaa atcacttggc
    31081 tgtacccaga aagcgggttc cagtgtcgtg gttgacgtgc tgcgttacgg cgagcgtctg
    31141 aaaacgcctg ggctgaactt gttaagtgcg ccgggtaacg atgccgtagc gaccagcgcc
    31201 ctggcgggtg cgggctgcca tatggtgctg ttcagtactg gtcgcggcac gccatacggt
    31261 ggatttgtgc cgacggtgaa aatcgccacc aacagtgaac tggcggcgaa gaaaaaacac
    31321 tggatcgact ttgacgcggg tcagctgatc cacggtaaag cgatgccgca gttgctggaa
    31381 gaatttatcg acaccatcgt tgagtttgcc aacggtaagc aaacttgcaa cgagcgtaac
    31441 gacttccgcg aactggcgat cttcaaaagc ggcgtaacgc tataaagtcg aaatgagttg
    31501 ttaataaaaa acggcgtttc ataatgtgaa gcgccgtttg cttttccata cctgataagg
    31561 atttatcatg accgcgtatt ggctggccca gggcgtgggt gtcatcgcct ttctgattgg
    31621 tatcacaaca tttttcaatc gtgacgaacg tcgcttcaaa aagcagcttt cggtctatag
    31681 cgccgttatt ggcgtacatt tttttcttct gggcacctat cccgctggtg ccagtgccat
    31741 ccttaatgcc attcgtacat tgattacctt acgcacgcgc agcttatggg taatggcgat
    31801 ttttattgtg ctgactggcg gaattggcct cgcgaagttc catcatcctg tcgaactatt
    31861 gccggttatc ggtacgattg tcagtacctg ggcgctgttc cgctgtaaag ggctcaccat
    31921 gcgctgcgtg atgtggtttt caacgtgttg ctgggtgatt cacaacttct gggcggggtc
    31981 gataggcggc acaatgattg agggtagttt tctgcttatg aatggcttga atatcattcg
    32041 tttctggcgg atgcagaaaa ggggaattga tccgtttaaa gtagagaaaa cccctcccgc
    32101 cgtagacgaa aggggttaaa caattaatta cgcagggcgc tatttgccag acgatcgtct
    32161 tccgcctggc aagctgccgc agtgaacagc acgtcagttg aagagttcag cgcggtttcg
    32221 caagagtcct gcaatacgcc gatgataaag ccgacggcaa ccacctgcat ggcgatatcg
    32281 ttcgaaatac cgaacatatt acaggccagc gggatcagca gcagagaacc ccccgccacg
    32341 ccggatgcgc cacaggcaca cagagaagcc accacgctca acagcagcgc cgtgggcaga
    32401 tcgaccggga tacccagcgt attaaccgca gccagcgtca acacggtaat ggtgattgct
    32461 gcgcccgcca tattgatggt ggcacccagc ggaatagaaa cggaataggt atcgcgatcc
    32521 agattcagct tttcacacag tgccatattc accggaatgt tcgccgcaga gctgcgggtg
    32581 aagaaggcat acacgccgct ttcgcgcagg cacagcagca ccagcgggaa cgggttacga
    32641 cgaattttcc accacaccag caatgggtta accaccagcg ccaccagtaa catacagcca
    32701 accagcacga ccagcagttg cgcgtagccc cacagtgtgg agaaaccggt ggttgccagg
    32761 gtagaagaaa ccagaccaaa aataccaatc ggtgcgaagc gaatgaccag tttcaccata
    32821 aaggtgacgg cattcgacat atcgttgacc aggtttttgg tggtctcgtt accgtgacgc
    32881 agtgcgaaac cgaggccaat tgcccacacc aggatcccga tgtagttacc tttcagcagc
    32941 gcgtcgatgg ggttggaaac catgctcatt accagcccgc gcatcacttc tacaatgcct
    33001 gacggtggcg aaatatcacc cgcgctactg gataagtgca gggtagaagg gaaggcgaag
    33061 ctgaaaacga ctgcggccag agcagcagag aaggtaccca gcagatagag gaacaaaata
    33121 gggcggatat tggttttctg cccgtgctgg tggttagcaa tagatgccat caccagcatc
    33181 aacaccagga tgggggcaac ggctttcagt gcgccgacga acaaagtacc taacagacca
    33241 acagcttccg ccgcgggttt tgatatccat gccagaagaa tccccagaac aaggccgacc
    33301 aggatttgtt ttaccaggct gccatgagcc agacgccgga atagccccgg tgaacgttgc
    33361 gtagtcattt ttcgatcctt tcattgtgtt atcgcacatc cctggtgcgt tctgtcgacg
    33421 catctttcag gatgtaacaa agtgtttgca aggtcgagta taaggaaagt tacagcttcg
    33481 ggaagcgaaa aatgctggat tttaaagaga agtcatattt tgaaacttaa tggttacatt
    33541 tgtttgacat aaacagaggg aaggactggc tactaaggct gattagataa catatgggca
    33601 aaactatttg gaatatgaga acggttgcgc tcctttaatg tgctatgcca tataaagtca
    33661 ctggcgcagg gagcgcgcag ggggcggcca atcgccgccg ccccctgcac ccccaggctc
    33721 tggcgaacaa aatcgccgct gcgcgattcc ctcagcttat cccttgcggc taccgggtcg
    33781 ggcgcaaggg aacgtccctg taagatgcgc cctcgccgcg catccatgcg cgtcgccccg
    33841 gcctccaggg aacgcttcgg cgattttgac gccaccaaca cccgtgcggt ctttttgtaa
    33901 agagctacaa gccgattaca taattaatag ctcacctttg tctggaatga gttatctcta
    33961 ggaatggctt aatatacagc gaatcattct ccccgctgct tatcatgccg ataattcacc
    34021 caggcgttga taataaacgt catcaccaga atgccaaaca ccacgcccag cgagacggcg
    34081 attggaatat ggtagaagtc gacaatcagc atcttgatac cgataaacac cagaatcacc
    34141 gccaggccat atttgagcat cgagaaacgc tctgctacgc ccgccagcag gaaatacatc
    34201 gcacgcaggc cgaggatcgc aaacaggttt gaggtcagca caatgaacgg gtcagtggtc
    34261 acggcgaaga tagccggaat gctatccacg gcgaaaatca cgtcgctcaa ttccaccaga
    34321 atcagtacca gcatcagcgg tgtggcgtac agcaatccat tcttacgcac aaagaaatgc
    34381 tcgttgtcga tggtgtcggt catgcgcaaa tgaccgcgta gccagcgcac cagcggcttg
    34441 tcgccaatgc ctgattcatc ttcatgggcg agcgccatct tcacgccggt aaacagcagg
    34501 aaggcaccga agatatacag gatccagtcg aactgcgaaa ttagccagct gccagtgaag
    34561 atcatgatgg tacgcagaac aatcgccccg agcacgccat acaccagcac gcggcgttgt
    34621 aatgccgccg gaacagagaa atagctgaac aacatcagcc agacaaagac gttatcgacc
    34681 gccagcgatt tctcaatcag ataacccgtg agaaaggcca gtgcctgtgg atcggcaacg
    34741 gcgcgacctt cagtttgcac cagataccac cagaaagcgg cgttaaacag taacgacagc
    34801 gtcacccaga ccagcgacca ggccgcagcc tgtttcatgg tcatggcatg tgccccacga
    34861 cgcccctgca acaacaggtc gatagccagc ataatggcga caacaacagc gaatccgccc
    34921 catagcaacg gcgtgccgac agtattcata gaagttcctt acacataaaa aaacggccaa
    34981 cgtcggaaga cgtcagccgc tgctttttat gcatagacct cgccttccgg caaggtctca
    35041 cttacaacaa aaaaggaata cgtctgcgta ttcctttcgg gttgcccggt gaccggatgt
    35101 ggtttttcac acatcgtaat gacgatcgac cggcaatgaa gttactcccc tttgcaggta
    35161 acaaagtatg acagaccatt cagccagtca atggtctgtt acataccttt tacactgttt
    35221 tactttggca attatagttt tacgctatct gccggaaaaa tcaccccagt ctggcggcgg
    35281 atctcagtca gcagtttggc ggtgatgcga ctgaccgcca gccctggatg atccaccaga
    35341 tgctcatcca ccagggtagc aaacagctct gcttcgtaga gcatggtatt aatatgctgc
    35401 ggctgggtga gatcctgcat ctggctgcca cgcggcacga agcacacttt ctggcattca
    35461 gacagttttt caatcaccag cgatcccgct tcgccctgaa tctcgctttc caggacagaa
    35521 tcactgacct ttgagtgctg caacgtaacg ctgaaatcac cgtaatccat caccaccacg
    35581 ccgtgggcat ctacgccgct tgccaacaaa ctggcggttg cctgcacgct tttcggttca
    35641 ccgaataatg ccaccgccga cgccaggcag taaaagccga tatccataat tgaaccgtta
    35701 gagaatgccg gattaaaggt gttgggattc tcaccatcca ggtaacgttg ataccgcgag
    35761 gaatactggc aatagttgaa aaagacttta cgcaatttgc cgactttcgg cagcgcctgg
    35821 cgcagcaaat gaaagttcgg caggctggcg gttttaaatg cctcaaacag caccacctga
    35881 ttttcccgcg cacaggcaat ggcggcatcc acttccgcca gattcgacgc cagtggtttc
    35941 tcgcaaatca catgaatttt atggctaagg aaaagttgtg tctgggaaaa gtgcagggca
    36001 ttcgggctgg caatatacac cgcgtcaatg gcatcgcttt ccgccatcgc ttccagcgag
    36061 gtaaacagat gctcgacaga aaaatcattg gcgaagtgct gggcctgttc aaggctgcgg
    36121 gaatatacgg cggttaactt gtatttaccg ctctcatggg cggcctcgac gaactggcga
    36181 gtgatccagt tcgtaccaat cacagcgaaa cgtatcataa cgcgtggata ctccataaag
    36241 ggattctgtc gtcaatgtag cacgcctttt tcatcttcga cgtgcgcaga tacgcattat
    36301 tctttcgccg gacagccatg ctcactaaac ggcccaatca tgaccgatgg cccgagatat
    36361 ttcggctggc gatggaggat tttggcgtca atcaccgcgc tgacacgagc gagtttctcc
    36421 cgcagttgag tataaaaacc gtgcttcata ttaaggtctg gcgcgttaaa cccgatagcg
    36481 tcgatgccgt actgttttgc cagccagatg gcgcgttggt tatgaaattc ttgcgagata
    36541 atggtgatat ggttttcccc aaacaccttt ttggcacgca ccactgaatc aagcgttgag
    36601 aatccggcat agtcgcagaa gatcactttc gccggtacgc ctttagcgat caaagcctgc
    36661 tgcatacctg aggcttcatc gtaattttta cgtccgttat cgccactgac taacagccat
    36721 ttcacttttc cgacgtggta caacgctgct gcggtatcaa tacgtcgggt aaagtagcga
    36781 ttacccggcc tcgcccccag caataaaccc acgttacgcg ccggaactgc gttgacatcg
    36841 ctccaggtca gctgcttgct ggcgttgacc attacgcgat cggcgataaa aatggtcgct
    36901 cctgccacaa gtaacagtaa gcaggtaatc tttagagtcc ggcgagaaaa acaaatacgg
    36961 agaagaaggc ggggctgcat ggtcgttatc cttaacgaaa ggggggaggt cgattttagg
    37021 cgctaaagaa agaaaagata acgcctgaca tcattttcag attttgtcct gcccaagcga
    37081 caactgcgtt agccacagcc gggtgtcgaa ctcaagctgg tggtactgcg gctccatatg
    37141 gcagcacaac tggtaaaacg ctttgttatg ctctttctcc ttcaggtgcg ccagttcgtg
    37201 caccacgatc atgcgtaaaa acggttccgg cgcgttgcga aacacagtag cgacgcggat
    37261 ctctgctttt gctttcagct tgccgccctg cacacgagaa acagcggtat gtaatccgag
    37321 cgcgtttttc agtacgtgga tcttattgtc atacatcact ttagtgatcg gcggggcatt
    37381 gcgcagaaac tgatttttta gatcctgggt atattgccag agggctttat cggtggcgta
    37441 gtcgtgtgtt cccggatagc gttttgccag cacatcgccc agacgctgtt cgttaatcag
    37501 cgttcgcacc tgggaaagta gctgctcggg ataaccctga agataagtaa gattgctcat
    37561 aaccgcccac gaaaaagaga aaaagggtat actcacgcac ccttttcagg ggcaacgtcg
    37621 aaattttatc attcaggagg gccgatgagc cacttagaca acggtttccg ttcactgaca
    37681 ctacaacgtt ttccggcgac ggatgacgtt aacccgctac aggcgtggga agcggcggat
    37741 gaatatttgc tgcaacagtt ggacgacaca gaaatccgcg gcccggtgtt gatcctgaat
    37801 gatgcctttg gtgcgttaag ctgcgcgctg gcggaacata agccgtacag cattggcgac
    37861 tcatacatca gtgaactggc gacgcgcgag aatttacgcc tcaacgggat tgatgagtcg
    37921 agcgtgaagt ttctcgacag caccgccgac tacccgcaac agccgggtgt ggtgctgatc
    37981 aaagtgccga aaacactggc attgctggaa cagcaactgc gtgcgctgcg caaagtggtc
    38041 acgtcggata cacgtattat tgccggtgcc aaagcccgtg acattcacac ttccacgctg
    38101 gagctgttcg agaaagtgct cggcccgacc accactacgc tggcatggaa aaaagcgcgc
    38161 ctgattaatt gcactttcaa tgaaccgccg ctggccgatg cgccgcagac cgttagctgg
    38221 aagctggaag gtactgactg gactatccac aaccatgcga atgtcttctc ccgcaccggg
    38281 ctggatattg gcgcacgctt ctttatgcaa catctgccag agaatctcga aggtgagatt
    38341 gtcgatctcg gttgcggtaa tggcgttatt ggtctgacgc tgcttgataa aaaccctcag
    38401 gcgaaagtgg tgtttgtcga tgaatcgccg atggcggttg cttccagccg tttgaacgtt
    38461 gaaaccaaca tgccagaggc gttagatcgc agcgagttta tgatcaacaa cgcgctctcc
    38521 ggcgtggagc ctttccgctt taatgctgtg ctctgcaacc cgccgtttca ccaacaacat
    38581 gcgctgaccg ataacgtcgc ctgggagatg ttccaccatg cccgccgctg cctgaaaatc
    38641 aacggcgagc tgtatattgt tgccaaccgt cacctggatt acttccataa actgaagaag
    38701 attttcggca actgcaccac catcgccacg aataataaat ttgtggtgct gaaagcagtg
    38761 aagctggggc gtcgtcggta agattgggag ttacctgccg gatgcggcgt taacgcctta
    38821 tccggcctac gttcagcacg ttactcccag gccggataag acgcggcaag cgtcgcatcc
    38881 ggcaatgtct gcccagttct ggaatcgcgc ttccataaaa caaaaaatcc ctctcaccat
    38941 cctctacatt ctcttgttta gcgtttttct acgtttattc ttccgtcaca cagataaatt
    39001 catccgttgc caatctgtca acggatgtta tcatattgcc aatttgtcaa cggacgtgat
    39061 gaagaatgca cctgataact caaaaagcat tgaaagatgc tgcggaaaaa tacccgcaac
    39121 ataaaacgga gttggtggct ctggggaaca cgattgctaa gggatatttc aaaaaacctg
    39181 agtcattaaa agcagtattc ccatctctgg ataacttcaa atatctggat aagcattatg
    39241 ttttcaatgt tgggggcaat gaattacgtg ttgtagcaat ggtctttttt gaatcgcaaa
    39301 agtgctacat acgtgaagtt atgacgcata aagaatacga tttctttacc gctgttcatc
    39361 gtactaaggg gaaaaaatga ttgcgattgc cgacatcttg caagcaggag aaaagctaac
    39421 tgcggtggca ccttttctgg cgggtattca gaacgaggaa caatacaccc aggcgctgga
    39481 actggtagat catctgctgc tcaacgatcc tgaaaacccc ttgctggatc tggtgtgtgc
    39541 caaaataacc gcgtgggaag aatcagcgcc cgaatttgcg gaatttaatg ccatggctca
    39601 agccatgcct ggcggtatag ccgtgattcg tacccttatg gatcaatatg gtttaaccct
    39661 tgccgatctg ccggaaattg gcagtaaatc tatggtgtca cgcgttttga gcgggaagag
    39721 gaaattaacg ctggaacacg ctaaaaaatt ggcaacgcga ttcggcattt ctcccgcctt
    39781 gtttattgat taacgcgacg tgccggatgc gttgcatccg gcgcttaaaa taacgattta
    39841 aatctccagc gccagccgtg ttccctgggc aattgcccgt cgtgcgtcca gctccatagc
    39901 cacatcgcag ccgccaatta aatgcacagt tttcccgcta tcaatcagcg gttgcgccag
    39961 cgcgcggttt ggctcttgcc ctgcgcagat caccacattg tccactgcta atacctgcgt
    40021 ttcgccgttg atcaccacat gcagcccgtc atcgtcaatc ttctgataac ttacgcctgg
    40081 gatcattttc acaccccgcg agagcagggt ggtgcgatgg atccagccgg tggttttgcc
    40141 taacccctgt cccggtttgc tggctttgcg ctggagcatc acaatttgcc gtgggctacg
    40201 cgggatctgc attccctgcg ggcttaagcc accagcctgt tgtaggctgc tgtcgatccc
    40261 ccactcatta cagaacctgg cgatattctg gctggtggat tcgcccggct gacttaaata
    40321 catcgccgta tcaaaaccaa tcccgccaca accgatgatg gcaaccttgt tgcctaccgg
    40381 cgctttgtcg cgcagtacat tgagataact caataccttc ggatgatcga tcccgtcgat
    40441 aggcggaacg cgcggcacga tcccactggc gaggatcgtt tcatcgaacg cctgtaactg
    40501 atccgccgtc acggtgtgat tgagtttcag cgtcacgccc gtcacttcga tcatccggtg
    40561 gtaatagcgc agcgtttcgt aaaactcctc tttgccgggg atctgtttgg cgatattaaa
    40621 ctgcccgccg atctcgctgt gggcatcaaa caatgttacc tgatgcccac gcgccgccgc
    40681 gttaatggca aacgccagcc cagcaggtcc cgcaccgacc accgccagat tttttttctg
    40741 cacggcagga aggattggca ttttggtttc gtggcaggcg cgaggattca ccaggcacga
    40801 ggtgactttg ccaacgaaga tctgatcgag gcaggcctga ttgcagccaa tgcaggtgtt
    40861 gatctcatcg gctcgccccg attgcgcttt tgacagcagt tccgcatcag caagaaacgg
    40921 tcgtgccatc gataccatat cggcatcgcc gcgcgagaga atatcgtcgg caacctgcgg
    40981 atcgttaatc cggttggtgg ttaccagcgg cagcgagacg tggcctttca gtttgcgcgt
    41041 gacccagcta aatgcgccgc gcggcacggg cgtggcaatg gtcggaatac gtgcttcatg
    41101 ccagccaatg ccggtgttga taatggtcgc gcccgccgct tcaatggcct gcgccagctc
    41161 taccgtttcg gcaaaagtcc cgccgtcttc gaccaggtcg agcatcgaca gtcggtagat
    41221 aataatgaag tcgttgccga cgcgttcgcg caccgcacgc actacttcta cggcaaatcg
    41281 catccggttg cggtaatcgc cgccccactg gtcactacgc tgattggtgc gcagcgtcag
    41341 aaattcgttg atcaaatacc cttcggaccc cattacctct acaccgtcgt atcctgcctc
    41401 ccgtgccagt tgcgcgcagt gggcgaaatc gtcgatcagt tgcaggatct cttcatgggt
    41461 gagttcgtga ggaacgaaac ggttgatggg ggcctgcaat gcggagggag cgaccagatg
    41521 cggttggtag ctgtagcgcc cggtatgcaa aatttgcagg gctattttgc cgccttcctg
    41581 atgtaccgct tcggtaatgg tgcgatggtg tgggatctgg ctggcgtcgt tgagcattgc
    41641 gccaccttcc atgccaacgc ctgttaaatc tggtgcaatg ccgccgctga caatcagcgc
    41701 cacgccgtga cgggcgcgtt cggcataaaa cgctgccagc cgctcggcac cgtccgggta
    41761 ttcctccagc ccggtgtgca ttgagcccat caacacgcgg ttttttaacg tggtaaaacc
    41821 taaatccagc ggggcgaaca gcgacgggta gctcataaaa ttgtccagta tgtaaaataa
    41881 ttgttatgtg gtcggatgag ttctaattta gccgtcgatg gagaaaaggg aaaaggggag
    41941 tgcggggttt gtgatgggat tcaaaaagaa tacgcccggt ggtaacgccg gatgcaacgc
    42001 ttgccgcgtt ttatccggcc tgggggattg cacatgtagg gcggataagg cgttcacgcc
    42061 gcatccgcca gccgttgcaa attactgctt acggaaaaaa tcgttataca acatatacaa
    42121 atgagcggca ctccaggaga aatttggtgc gccttgctgt gcgccagtca gcgggttgta
    42181 attctcctga attgggccat cggcggttaa tcctttggcg tgccggaaga acgtatccgc
    42241 cagcttcagg gcatcatcac gataaccgta acgctccatc cctttcagac caaaccagaa
    42301 ctgatccacc catacgcgcc cacgccagta gatatcagcg ccaaaagccg gattggttaa
    42361 cgccgccgtt cccagcggga caaaggtgtt gaactcttta ggatcgagca tcaccttcac
    42421 caccgcgtcg gcgttggcct gcgttgccgc accgttaaac agcggcgacc agccttccgg
    42481 ccctttaccg cgctcaacaa tcggtttgcc cgcgcagccg ttcgccagtg gtttatcttc
    42541 aatacgtacg tcatagtaga actgggtagt cgggtcgaac atacaggtgt tgatgtagtc
    42601 cgcgagctgc tgtgccaact ggcgatagcg tttggcctct tctggcttac cgaggatggt
    42661 tgccatctcc gccagataat ggttatcgct gtacatatag ctggcctgat ccaccgactc
    42721 ctgcaatagc gagtagccca gcaacgttcc gtcctgactg cggttttcgg cgaatttcac
    42781 cgtccagtcg ctacgtttgc cgccattagc gacatattta tccagctgtt ctttgtcgat
    42841 aaacccaaag acggcggcat catcacgccc cgattcccac gatgcagcaa cctgtgccgg
    42901 aatttccaga ctgtcgtaca ggcctttctc caccacgcgg gcgtagttgt tcagcccaga
    42961 ctgcgtctct tctttatcgc cttttttcac cgtaaacagc atctcgccgc tctcagtgtt
    43021 gtgggctttg tcgcgggtcg cgccatattc cggcacgccg ttgccgttgt gatcgcggtt
    43081 acgtaaccac cagtcgtgat aggccaccag tttcgggtac atctctgcca accaggcttt
    43141 atcctgggtg acgttgtaca cttccatcac cgaccaggcg gcaaggctgg gtttggtatt
    43201 acgttcgttc cagttgccgc catcgccacc acgctcgggg ctaaggttcc acgctatcag
    43261 gtcggggaca aagcccacat cctgcggacg cacgctatcg ccaggctgga tctgccagga
    43321 gaagaccgcg cggatattct ctttggcgat gtccggattg aaatgcgcca tcgcaaacgc
    43381 ctgcttccag gtatcccacg gccaggtctg attgccggag aaccagcgcc cggtcaccga
    43441 cggtgtaacg gtgttaaatt tcaccgcacc gcccggtgag agccagttac cgttgagcgt
    43501 ttcgatcgct ttcaccgcga cgcgcgtctg ttccggcgtc gcatccggat tggttaaccc
    43561 tttcttcagg tattcttccc agcgttgctg cgaggcggtg agataaaacg ccggacgcgc
    43621 cagaatatcg cggatctgca tttgctcttt gctaacttcc tgagcggtca gcagatggga
    43681 ataggtggtg tagagcgtgg tcgaaccgtt gatatgcgcc ttactggtaa agcgattacc
    43741 gttgatttca gtctgcaccg gcagggattt atgcacctga tattccgatt cgccggaggt
    43801 cagcagatcc caggtggcgc gcactttgcc aaaggtgact ttcaggccat cacgggtggc
    43861 gctgattttg cgctgatagt cagggtattc gccagcaatg gttttatcgg aaagcggttt
    43921 cccttctttc gcttccagtt tttccagcag ttcgccgtcc cacaccagat ccagcggttt
    43981 attgctggtg attttggttt ccagtagtga cgtgcgcggc gtggcgaagc gcagagtcat
    44041 ttcgacttgc acatcttttg ctgtcagttt ttgcaccagc gcaccgggaa tactgtatgc
    44101 ctccagcgta aagtcgactt tcttgccgtc ctgccagacg gtcaggcggt cgaaattgct
    44161 ggccataaag ttgatgtact cttccgtcag cagcgcaacg cccggaaagc cgcccatggt
    44221 gttagggccg tctggcaaca gatgaccatg ccaggctccg agatcgaaaa acggattaaa
    44281 acgctggtga tcgtcgtaat cgtaatcctt catgtactgc ggcgcgccag tacggttaat
    44341 cacgttttta taattgtcgg cgttagcggc atgggcggaa aaacttatca gcagagcgca
    44401 ggttactggc gttaaaatag tttttatttt catgaaatta cagtccttat taccagtaga
    44461 aatattgcat caggaagatt tggtcgctgg gatcgtgaga ggcatttttc atgtagaagc
    44521 ggctatcaaa agccgtggcg aaacgtccgc cgccgtattc gtaccagata ccaaactgca
    44581 tgaaagaggt ggtttcatcg tcagcgtggt cagggcgatg tttggcgtag gtgtaagcgg
    44641 tggagagata aacgcccttt aacgcttcat acatggcgct caccatcatc ccactttcac
    44701 ttcccggtac gtccagcccg tcggcgcgtc cggtatggtg ccaggcgcga gcggcaaagt
    44761 taggagttag caggcccgtg agatagagct gtcctgtgcc gtcggtgatt tccagaccgt
    44821 tcatccaggt gatgccttcg gcaaggtcat actggatgta accgttcatc attgccggat
    44881 aggtgtattt gtcgtcatag cgatcgtatt tgccaaaatg tagccaggct ttgctttcat
    44941 catgatgtcc ggcaggagtc gcggtgacgc tataacgaaa gttgccgctt aagttctgta
    45001 ccttcagcgc agtcataatg tcgcgggtat tgggaatgac gtagcccaga tcgggggtga
    45061 aatcgcccca ccattgcagg tcatccagcg aggaatcctg acgcagactg agcatgattt
    45121 cggtgccatc attggtacga taaccgccat agaaacggtt aattccgccc tcgaaaccac
    45181 cccagctatg gtctggcacc caggcattgc cgtcgtggtc ggcctgcacg gtccagcctt
    45241 cgccgtaaat caggccgaac caggagccgt acttcatctc caggccacct tcaatatagg
    45301 taccgtcgct gtatttatgt ttattctcac cctctaaatc catatatcca ccacccccta
    45361 atccctaatt cgccgtaaaa acgaaatgaa ggagaagatg tcgaggtaat tgcgggtgtt
    45421 tttgtgtcgt catgttctgc cggacgtgct tcttctcctg ccgcagaaaa ggcgtagcaa
    45481 aacggcagag caagtaatgc tctgcatggt gcagtgatta atttcattgt gttaatccat
    45541 gagctaaaga aaaagtacag ccttttatcg ttgtaattat aaaaatataa aggcagaaac
    45601 tattttccct ctcctcatcc aggaagaggg aaaatgcctg ctcatttttt attattctgc
    45661 ttaatcaaca ttgctggctg gcgtcgcttc aatatggcgc tcttcagccg ttaatccctt
    45721 tatatattta ctgtatttcc accacgcaaa gccaaggaag ataacaatac cgccgacgtt
    45781 ataaaaaatg atggtcagaa tattcgcgcc agtcgggaat gtcgaagcga caaaccctac
    45841 ggcaaatatc gcaatcagca ttgaaacaac gataataccg gtgcggcggg agcccatacg
    45901 gaaatcgcgt ggcaggtgat ctaatttggc gcgtaaattc aggtaagcca gcatgatgaa
    45961 taacggcgga agcatggacg ctg
//
LOCUS       DAESHI010000022        44084 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.022, whole genome shotgun sequence.
ACCESSION   DAESHI010000022 DAESHI010000000
VERSION     DAESHI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44084
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.022"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..296
                     /locus_tag="KW011_001265"
     CDS             <1..296
                     /locus_tag="KW011_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HBH7659653.1"
                     /translation="LVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISI
                     PVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD"
     gene            791..1690
                     /gene="lysO"
                     /locus_tag="KW011_001266"
     CDS             791..1690
                     /gene="lysO"
                     /locus_tag="KW011_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HBH7659654.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1834..3486
                     /gene="hcp"
                     /locus_tag="KW011_001267"
                     /gene_synonym="priS"
     CDS             1834..3486
                     /gene="hcp"
                     /locus_tag="KW011_001267"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBH7659655.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            3498..4466
                     /gene="hcr"
                     /locus_tag="KW011_001268"
     CDS             3498..4466
                     /gene="hcr"
                     /locus_tag="KW011_001268"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HBH7659656.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            4599..6317
                     /gene="poxB"
                     /locus_tag="KW011_001269"
     CDS             4599..6317
                     /gene="poxB"
                     /locus_tag="KW011_001269"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HBH7659657.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            6354..7355
                     /gene="ltaE"
                     /locus_tag="KW011_001270"
     CDS             6354..7355
                     /gene="ltaE"
                     /locus_tag="KW011_001270"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBH7659658.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            7366..8796
                     /locus_tag="KW011_001271"
     CDS             7366..8796
                     /locus_tag="KW011_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HBH7659659.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            8895..9908
                     /locus_tag="KW011_001272"
     CDS             8895..9908
                     /locus_tag="KW011_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBH7659660.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9905..10735)
                     /gene="amiD"
                     /locus_tag="KW011_001273"
     CDS             complement(9905..10735)
                     /gene="amiD"
                     /locus_tag="KW011_001273"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HBH7659661.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10732..11055)
                     /locus_tag="KW011_001274"
     CDS             complement(10732..11055)
                     /locus_tag="KW011_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBH7659662.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(11266..13035)
                     /locus_tag="KW011_001275"
     CDS             complement(11266..13035)
                     /locus_tag="KW011_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBH7659663.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENMPSANYQFNTFFSNAFETDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQDQLDKHFTIESHPLLYRHT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKGAFIHFYDYSRRYAISENIEKDAVTNNIA
                     LMNTFYDSDNTSGEVLNNDFTEEEPFETALDVEHTIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMRSSHYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGFFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNIINNVFKNVSFRSVKINNVNTHSLKFINCHFFNVDMIRVNFSKCLFH
                     DCSMHGVKIKPWLPVKWTKELINDYLYGCLLSLYSICARDIYNMSAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQERAFIYEFARMIFAYINDKSIEILLLSCFAASDQKAIQRY
                     IPQSQDGEDFRSHLQYKLPLSAH"
     gene            13684..14199
                     /locus_tag="KW011_001276"
     CDS             13684..14199
                     /locus_tag="KW011_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBH7659664.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            14417..15145
                     /gene="artP"
                     /locus_tag="KW011_001277"
     CDS             14417..15145
                     /gene="artP"
                     /locus_tag="KW011_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HBH7659665.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            15163..15894
                     /gene="artJ"
                     /locus_tag="KW011_001278"
     CDS             15163..15894
                     /gene="artJ"
                     /locus_tag="KW011_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH7659666.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            15901..16617
                     /gene="artQ"
                     /locus_tag="KW011_001279"
     CDS             15901..16617
                     /gene="artQ"
                     /locus_tag="KW011_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HBH7659667.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            16617..17285
                     /gene="artM"
                     /locus_tag="KW011_001280"
     CDS             16617..17285
                     /gene="artM"
                     /locus_tag="KW011_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HBH7659668.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            17577..18308
                     /gene="artJ"
                     /locus_tag="KW011_001281"
     CDS             17577..18308
                     /gene="artJ"
                     /locus_tag="KW011_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HBH7659669.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(18483..19610)
                     /gene="rlmC"
                     /locus_tag="KW011_001282"
     CDS             complement(18483..19610)
                     /gene="rlmC"
                     /locus_tag="KW011_001282"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HBH7659670.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(19651..20139)
                     /locus_tag="KW011_001283"
     CDS             complement(19651..20139)
                     /locus_tag="KW011_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HBH7659671.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(20199..21044)
                     /gene="potI"
                     /locus_tag="KW011_001284"
     CDS             complement(20199..21044)
                     /gene="potI"
                     /locus_tag="KW011_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HBH7659672.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(21041..21994)
                     /gene="potH"
                     /locus_tag="KW011_001285"
     CDS             complement(21041..21994)
                     /gene="potH"
                     /locus_tag="KW011_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HBH7659673.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(22004..23137)
                     /gene="potG"
                     /locus_tag="KW011_001286"
     CDS             complement(22004..23137)
                     /gene="potG"
                     /locus_tag="KW011_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HBH7659674.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(23232..24344)
                     /gene="potF"
                     /locus_tag="KW011_001287"
     CDS             complement(23232..24344)
                     /gene="potF"
                     /locus_tag="KW011_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HBH7659675.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(24695..25171)
                     /locus_tag="KW011_001288"
     CDS             complement(24695..25171)
                     /locus_tag="KW011_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HBH7659676.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(25259..26161)
                     /gene="rimK"
                     /locus_tag="KW011_001289"
     CDS             complement(25259..26161)
                     /gene="rimK"
                     /locus_tag="KW011_001289"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HBH7659677.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(26222..26944)
                     /gene="nfsA"
                     /locus_tag="KW011_001290"
     CDS             complement(26222..26944)
                     /gene="nfsA"
                     /locus_tag="KW011_001290"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HBH7659678.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(26928..27215)
                     /locus_tag="KW011_001291"
     CDS             complement(26928..27215)
                     /locus_tag="KW011_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HBH7659679.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            27375..27632
                     /gene="grxA"
                     /locus_tag="KW011_001292"
     CDS             27375..27632
                     /gene="grxA"
                     /locus_tag="KW011_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HBH7659680.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(27662..28039)
                     /locus_tag="KW011_001293"
     CDS             complement(27662..28039)
                     /locus_tag="KW011_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HBH7659681.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            28309..29994
                     /locus_tag="KW011_001294"
     CDS             28309..29994
                     /locus_tag="KW011_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HBH7659682.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(30169..30705)
                     /gene="rcdA"
                     /locus_tag="KW011_001295"
     CDS             complement(30169..30705)
                     /gene="rcdA"
                     /locus_tag="KW011_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HBH7659683.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            30789..31997
                     /locus_tag="KW011_001296"
     CDS             30789..31997
                     /locus_tag="KW011_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH7659684.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            31997..32812
                     /gene="ybjI"
                     /locus_tag="KW011_001297"
     CDS             31997..32812
                     /gene="ybjI"
                     /locus_tag="KW011_001297"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HBH7659685.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            32898..33182
                     /locus_tag="KW011_001298"
     CDS             32898..33182
                     /locus_tag="KW011_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659686.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTGDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(33223..34450)
                     /gene="mdfA"
                     /locus_tag="KW011_001299"
                     /pseudo
     CDS             complement(33223..34450)
                     /gene="mdfA"
                     /locus_tag="KW011_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            34735..35331
                     /gene="ybjG"
                     /locus_tag="KW011_001300"
     CDS             34735..35331
                     /gene="ybjG"
                     /locus_tag="KW011_001300"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBH7659687.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            35389..36147
                     /gene="deoR"
                     /locus_tag="KW011_001301"
     CDS             35389..36147
                     /gene="deoR"
                     /locus_tag="KW011_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HBH7659688.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(36194..37396)
                     /gene="dacC"
                     /locus_tag="KW011_001302"
     CDS             complement(36194..37396)
                     /gene="dacC"
                     /locus_tag="KW011_001302"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBH7659689.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            37643..38268
                     /gene="gstB"
                     /locus_tag="KW011_001303"
                     /pseudo
     CDS             37643..38268
                     /gene="gstB"
                     /locus_tag="KW011_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(38265..39380)
                     /gene="yliI"
                     /locus_tag="KW011_001304"
     CDS             complement(38265..39380)
                     /gene="yliI"
                     /locus_tag="KW011_001304"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HBH7659690.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(39491..39874)
                     /gene="bssR"
                     /locus_tag="KW011_001305"
     CDS             complement(39491..39874)
                     /gene="bssR"
                     /locus_tag="KW011_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HBH7659691.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            40087..41412
                     /gene="rimO"
                     /locus_tag="KW011_001306"
     CDS             40087..41412
                     /gene="rimO"
                     /locus_tag="KW011_001306"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBH7659692.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(41459..42780)
                     /locus_tag="KW011_001307"
                     /pseudo
     CDS             complement(41459..42780)
                     /locus_tag="KW011_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(42788..>44084)
                     /locus_tag="KW011_001308"
     CDS             complement(42788..>44084)
                     /locus_tag="KW011_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH7659693.1"
                     /translation="SIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLA
                     VGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENV
                     QTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFS
                     EFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVS
                     AERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPW
                     QLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISID
                     DFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVE
                     SEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT        10951 a        11335 c        10769 g        11029 t
ORIGIN      
        1 cgctggtagt tgaactggta ttgagtgcag gtttcctgtt ggtgatccac ggcgcaaccg
       61 acaaattcgc gccggcaggt tttgcgccga tcgctattgg tctggcctta accctgattc
      121 acttaattag tattccggtg actaacactt ctgttaaccc ggcgcgcagc accgcggttg
      181 ctatcttcca gggcggctgg gcattagaac aactgtggtt cttctgggtg gtgccaattg
      241 tcggcggcat tatcggtggt ctgatttacc ggaccctgct ggaaaagcgt gattaattaa
      301 cgcagaaggc ctggcgtaat gtcaggcctt tttttaagat gttgttatat gttgattcat
      361 catcttgttg cgctcctttc atgagctaag ccacatattg ccactggcgc aaggagcgcg
      421 cagggggcgg ccaatcgccg ccgccccctg cccccccggg ctctggcgaa caaaatcgcc
      481 gctgcgcggt gccctcgact tatcccttac ggctaccggg tcgggcgcga ggtaacatcc
      541 ctgtaaaacg cgccctcagc ccacatccat gtgggctgcc ccggccttca gggaacgcct
      601 cggcgatttt gacgccacca acatcggtgc agtttgtaat taaagcgcaa gaatatcttt
      661 aaatgaaaac gaatactcta ctgctcctta tgcataacat ttccttttaa ttgcaacttt
      721 actcgtcctt ccgctttcag tagtgtgtca tcgcgcattt cgacttcatc tttgcaagga
      781 ctgggttttc atgttttctg ggctgttaat cattctggtt cccctgattg taggttacct
      841 cattccgctt cgccaacaag ctgcgttaaa agttattaat cagctattaa gctggatggt
      901 ttaccttatt ctctttttta tgggtatcag tctggcgttt ctcgataacc tcgccagtaa
      961 cctgttggcg attctgcatt attctgccgt cagtattacc gttattttac tgtgtaatat
     1021 tgccgccctg atgtggctgg agcgaggcct gccgtggcgc aaccaccatc agcaagaaaa
     1081 actcccgtcg cgtattgcga tggcgctgga gtcgctaaaa ctgtgcggcg tagtagtgat
     1141 tggttttgcc attggtctga gtggactggc tttcttacaa cacgcgaccg aagccagtga
     1201 atacacgtta attttgctac ttttcctcgt tggtattcag ttgcgcaata atggcatgac
     1261 cttaaagcag attgtcctta atcgccgggg aatgattgtc gccgtggtgg tggttgtcag
     1321 ttcattaatt ggtggtttaa ttaacgcctt tattcttgat ctccccatca ataccgcgct
     1381 ggcaatggcc tccggtttcg gctggtattc tctttccggt attttattga ccgaatcttt
     1441 tggtccggta atcgggagcg cggcgttttt taatgatctg gcccgtgaac tgattgctat
     1501 tatgttgatc cctgggctga ttcgccgcag ccgctctact gcactgggct tatgcggtgc
     1561 cacatcaatg gatttcaccc tgcccgttct tcaacgtact ggcgggctgg atatggtccc
     1621 ggcggcaatt gttcacggtt ttattcttag cctgttagtg ccgatcctca tcgccttttt
     1681 ctctgcgtaa tacctctctg gcggtagatc cctgccgcca aaattgcgct aaatcaatct
     1741 cccttaaagt tgcatgaaaa atccctttta tccccgcgtt aagcgtctta accttaaaca
     1801 tgtatattaa atataacttt aaaaggtgtg atcatgtttt gtgtgcaatg tgaacaaact
     1861 atccgtactc cggcaggaaa cggctgctca tacgcgcagg ggatgtgtgg taaaacggcg
     1921 gaaacttctg accttcagga tttactcatc gcggcgctgc aagggctttc tgcctgggcg
     1981 gtaaaagcgt gtgaatacgg catcatcaac cacgatgttg acagcttcgc gccacgtgcc
     2041 tttttctcaa ccctgaccaa cgttaacttc gattctccgc gtattgtcgg ctacgctcgt
     2101 gaagcgattg ccttgcgcga ggcgctgaaa gcacaatgcc tggctgtaga tgccaacgcg
     2161 cgcgtcgata acccgatggc tgacctgcaa ctggtgagcg atgatctcgg cgagctgcaa
     2221 cgtcaggcag cagaatttac ccctaacaaa gataaagcgg cgattggcga aaacattctc
     2281 ggcctgcgtc tgctgtgcct gtatggcctg aaaggtgcgg cggcctatat ggaacacgcg
     2341 catgttctcg gtcaatacga caacgatatt tatgcccagt accataaaat catggcgtgg
     2401 ctggggacct ggcctgccga tatgaacgcg cttcttgagt gttcaatgga aatcggccag
     2461 atgaacttca aagtgatgag cattctggat gcaggcgaaa ccggtaaata cggtcacccg
     2521 acgccaaccc aggtcaacgt caaagcgacg gcgggtaaat gcattctgat ttccggtcac
     2581 gatctcaaag atctctacaa cctgctggaa cagaccgaag gcacgggcgt taatgtctac
     2641 acccacggcg aaatgctgcc tgcgcatggc tacccggagc tgcgtaaatt caagcatctg
     2701 gtcggtaact acggcagcgg ctggcagaat cagcaagtgg agttcgctcg tttccctggc
     2761 cccatcgtga tgacctcgaa ctgcatcatc gacccaaccg taggcgctta tgacgatcgt
     2821 atctggaccc gcagcattgt tggctggcct ggcgtgcgtc atctggacgg tgatgatttc
     2881 tctgcggtta tcacccaggc gcaacagatg gcaggcttcc cgtacagcga aattccgcac
     2941 cttatcaccg tgggttttgg tcgccagacg ctgcttggcg ctgctgatac gctgattgat
     3001 ctggtgagcc gtgaaaaact gcgtcatatc ttcctgcttg gtggctgtga cggcgcacgc
     3061 ggcgagcgcc actacttcac cgatttcgcc accagcgtgc cggatgactg cctgatcctg
     3121 accctcgcct gtggtaaata tcgctttaac aaacttgagt ttggcgatat cgaaggtctg
     3181 ccgcgtctgg tagatgcagg tcaatgtaac gatgcttact cagcgattat tctggctgtc
     3241 actctggcag agaaactggg ctgtggtgtg aacgatctgc cgctgtcgct ggtgctctcc
     3301 tggtttgaac agaaagcgat cgtcattctg ctgacgctgc tttctctggg cgtgaaaaat
     3361 atcgtcaccg gcccgactgc acctggtttc ctgacaccag acctgctggc ggtgctgaac
     3421 gagaaattcg gcctgcgttc tatcaccact gttgaagaag acatgaagca actgttgagc
     3481 gcgtaaggag tttgtcgatg acgatgccaa cgaatcaatg cccgtggcgg atgcaggttc
     3541 atcacattac gcaagaaacg ccggatgtgt ggacgatttc cctgatttgc cacgattact
     3601 acccatatcg cgccgggcaa tatgcactgg tcagcgtgcg aaactcagcg gaaacgctgc
     3661 gtgcttacac catttcctcc acgccaggcg tgagtgaata tatcaccctg accgtgcggc
     3721 ggattgatga cggtgtcggc tcccagtggc tgacgcgcga tgtaaaacgc ggtgattatc
     3781 tctggctttc ggacgcgatg ggggaattta cctgcgacga taaagcagaa gataaattcc
     3841 tgttgctggc ggcaggctgc ggcgtcacgc cgattatgtc gatgcgtcgc tggcttgcga
     3901 agaaccgtcc acaggccgat gtgcgggtga tctacaacgt gcgtacgccg caggatgtta
     3961 ttttcgccga tgagtggcgt aactatccgg taacgctggt ggcagaaaat aacgttaccg
     4021 aaggctttat cgctggtcgt ctcactcgcg aactgctggc aggtgtacct gacttagctt
     4081 cacgtaccgt gatgacctgc ggcccggctc cgtatatgga ttgggtagag caggaagtga
     4141 aagcgctcgg cgtgacgcgt ttctttaaag agaaattctt caccccagta gcagaagcgg
     4201 cgaccagcgg tctgaaattc accaaactgc aaccggcacg agaattttac gccccggttg
     4261 gcaccacgct actggaggcg ctggaaagca ataacgttcc ggttgtcgct gcctgccgtg
     4321 cgggtgtttg cggctgctgt aagacaaaag tggtttccgg tgaatatacg gtgagcagta
     4381 caatgacgct gaccgacgcc gaaatcgctg aaggttacgt actggcctgc tcctgccatc
     4441 cgcaggggga tttggttctc gcataatcgc cttatgcccg atgatattcc tttcatcggg
     4501 ctatttaacc gttagtgcct cctttctctc ccatcccttc cccctccgtc agatgaacta
     4561 aacttgttac cgttatcaca ttcaggagat ggagaaccat gaaacaaacg gttgcagctt
     4621 atatcgccaa aacactcgaa tcggcagggg tgaaacgcat ctggggagtc acaggcgact
     4681 ctctgaacgg tcttagtgac agtcttaatc gcatgggcac catcgagtgg atgtccaccc
     4741 gccacgaaga agtggcggcc tttgccgctg gcgctgaagc acaacttagc ggagaactgg
     4801 cggtctgcgc cggatcgtgc ggccccggca acctgcactt aatcaacggc ctgttcgatt
     4861 gccaccgcaa tcacgttccg gtactggcga ttgccgctca tattccctcc agcgaaattg
     4921 gcagcggcta tttccaggaa acccacccac aagagctatt ccgcgaatgt agtcactatt
     4981 gcgagctggt ttccagcccg gagcagatcc cacaagtact ggcgattgcc atgcgcaaag
     5041 cggtgcttaa ccgtggcgtt tcggttgtcg tgttaccagg cgacgtggcg ttaaaacctg
     5101 cgccagaagg ggcaaccacg cactggtatc atgcgccaca accagtcgtg acgccggaag
     5161 aagaagagtt acgcaaactg gcgcaactgc tgcgttattc cagcaatatc gccctgatgt
     5221 gtggcagcgg ctgcgcgggg gcgcataaag agttagttga gtttgccggg aaaattaaag
     5281 cgcctattgt tcatgccctg cgcggtaaag aacatgtcga atacgataat ccgtatgatg
     5341 ttggaatgac cgggttaatc ggcttctcgt caggtttcca taccatgatg aacgccgaca
     5401 cgttagtgct actcggcacg caatttccct accgcgcctt ctacccgacc gatgccaaaa
     5461 tcattcagat tgatatcaac ccagccagca tcggcgctca cagcaaggtg gatatggcac
     5521 tggtcggcga tatcaagtcg actctgcgtg cattgcttcc attggtggaa gaaaaagccg
     5581 atcgcaagtt tctggataaa gcgctggaag attaccgcga cgcccgcaaa gggctggacg
     5641 atttagctaa accgagcgag aaagccattc acccgcaata tctggcgcag caaattagtc
     5701 attttgccgc cgatgacgct attttcacct gtgacgttgg tacgccaacg gtgtgggcgg
     5761 cacgttatct aaaaatgaac ggcaagcgtc gcctgttagg ttcgtttaac cacggttcga
     5821 tggctaacgc catgccgcag gcgctgggtg cgcaggcgac agagccagaa cgtcaggtgg
     5881 tcgccatgtg cggcgatggc ggttttagca tgttgatggg cgatttcctc tcagtagtgc
     5941 agatgaaact gccagtgaaa attgtcgtct ttaacaacag cgtgctgggc tttgtggcga
     6001 tggagatgaa agctggtggc tatttgactg acggcaccga actacacgac acaaactttg
     6061 cccgcattgc cgaagcgtgc ggcattacgg gtatccgtgt agaaaaagcg tctgaagttg
     6121 atgaagccct gcaacgcgcc ttctccatcg acggtccggt gctggtggat gtggtggtcg
     6181 ccaaagaaga gttagccatt ccaccgcaga tcaaactcga acaggccaaa ggatttagct
     6241 tgtatatgtt gcgcgcaatc atcagcggac gcggcgatga agtgatcgaa ctggcgaaaa
     6301 cgaactggct aaggtaaaaa gggtggcatt tcccgtcata ataaggacat gccatgattg
     6361 atttacgcag tgataccgtt acccgaccga gccgcgccat gctcgaagca atgatggccg
     6421 ccccggttgg ggacgacgtt tacggagacg accctaccgt taatgctctg caggactacg
     6481 ccgcagagct ttccggtaaa gaagccgcca tttttctgcc gaccggcacc caggctaacc
     6541 tggtcgctct gctcagtcac tgcgaacgcg gcgaagagta tattgtcggt caggccgcgc
     6601 ataattatct gtttgaagcc ggtggcgcag cggtgctggg cagtattcaa ccgcaaccca
     6661 tcgacgcggc tgccgacggc acgctaccgc tggataaagt ggcgatgaaa atcaaacccg
     6721 acgatatcca tttcgcccgc accaaattac tcagtctaga aaacacccac aacggcaaag
     6781 tgctgccgcg tgaatacctg aaagaagcat gggaatttac ccgcaagcgc aatctggcgc
     6841 tgcatgttga cggtgcgcgc atctttaatg ccgtggtggc ttacggctgc gaactgaaag
     6901 agatcacgca atattgtgat tcgttcacca tttgcctgtc gaaaggtctt gggacgccag
     6961 tcggttcatt actcgtcggt aatcgtgatt acattaaacg tgccatccgc tggcggaaaa
     7021 tggccggtgg cgggatgcgt cagtccggga ttctggctgc cgccgggatg tatgccctga
     7081 aaaataacgt cgcacggttg caggaagatc acgacaacgc cgcctggatg gcggagcaac
     7141 tgcgtgaagc aggcgcggat gtgatgcgtc aggacactaa tatgctgttt gttcgcgtcg
     7201 gggaagaaaa tgctgccgcg ttaggcgaat acatgaaagc gagaaacgtg ctgattaacg
     7261 cctcgccgat tgtccgcctg gtgacgcatc ttgacgtctc gcgcgaacaa ctggcggaag
     7321 tcgccgccca ctggcgcgca ttcctggcgc gttaaggaga gagacgtgcc gcaacgcatt
     7381 ttagttctcg gtgccagtgg ctacattggt cagcatctgg tgcgcacact cagccagcaa
     7441 gggcatcaga tcctggcggc ggcacgtcat gtcgacaggc ttgcaaagct gcaactggca
     7501 aacgtcagtt gccataaagt cgatctcagc tggccggata accttccggc cctgttgcag
     7561 gatatcgata ccgtctattt tctggtgcac agcatgggcg aaggcggcga ttttatcgct
     7621 caggagcgcc aggtggctct caacgtccgc gatgcgctac gtgaagtacc agttaagcaa
     7681 ttaatctttc tcagttcgtt gcaggccccg ccacatgagc agtcggatca tctgcgtgct
     7741 cgtcaggcta cggcggacat tcttcgtgaa gcgaatgtac ctgtgaccga acttcgggcc
     7801 ggaattatcg ttggcgcagg ttcagcggcg ttcgaagtca tgcgcgatat ggtctacaac
     7861 ctgccagtgt taacgccgcc acgctgggta cgttcacgca ccacgcccat cgcgctggaa
     7921 aacttgctgc actatctggt ggcgttgtta gatcatccgg ccagcgaaca ccgcatcttc
     7981 gaagccgccg gaccagaggt gctcagttat cagcaacagt ttgaacattt tatggcggtg
     8041 agcggtaagc gccgctggtt gatccccatc cccctcccca cccgctggat ttcggtgtgg
     8101 tttctcaatg tgattacttc cgtaccgccc accaccgcca gggcgttgat tcaggggctg
     8161 aaacacgatc tgctggcgga tgataccgcg ctacgtgcac tcatcccaca acggctgatc
     8221 gctttcgatg acgcggtacg tagcacgttg aaagaggagg aaaaactggt caactccagc
     8281 gactggggct acgacgctca ggcctttgcc cgctggcgac cggagtacgg ttattttgcc
     8341 aaacaggcgg ggtttaccgt taaaacgtcc gccagccttg ctgctttatg gcaggtagtg
     8401 aaccaaatcg gcggtaaaga gcgttatttc tttggcaata ttttgtggca gacacgggcg
     8461 ttgatggacc gcgcgatcgg tcataagctg gcgaaaggcc gcccggagcg cgaatattta
     8521 cagactggcg atgcggtgga tagctggaaa gtgattgtcg ttgaaccgga aaaacaactt
     8581 acgttgttat ttggcatgaa agcgtcgggg ctgggacgac tgtgttttag cctggaagat
     8641 aaaggcgact atcgtactat cgatgtccgc gctttctggc atccgcacgg tatgccgggg
     8701 ctgttttact ggttattgat gatccccgcg catctgttta tttttcgcgg aatggcaaaa
     8761 caaatcgcca gactggcaga acaaagcaca gattaatcga caaaacctgc gttattcttt
     8821 catgtttccc attgcatcat gggggaaatc acggaagaat gcgcacgtca ttttctcaac
     8881 aacagtggat tgatatgaag gtactggtta ccggcgccac cagcggctta ggtcgaaacg
     8941 cggtagagtt tttatgccag aaaggcatca gcgtgcgagc gaccggtcgc aacgaggcaa
     9001 tgggcaaatt gctggaaaaa atgggcgcag agtttgttcc ggcggatctg accgagctgg
     9061 tttcatcaca agctaaagtg atgctcgcgg gcattgatac gctgtggcac tgctccagct
     9121 ttacctcacc ctgggggaca caacaggctt tcgatctggc taacgttcgc gccactcgcc
     9181 gcctgggtga atgggctgtc gcctggggtg tgcgtaactt tattcatatc tcttccccct
     9241 ccctgtactt cgattatcac caccatcgcg atattaaaga agattttcgc cctcaccgct
     9301 tcgccaacga gtttgcccgc agcaaagcag ccagcgaaga agtgatcaat atgctttcgc
     9361 aggcgaatcc acaaacgcgc tttactattc tgcgcccaca aagtctgttc ggaccgcacg
     9421 ataaagtctt tattccccgt ctggcgcata tgatgcacca ctacggcagt attctgttac
     9481 cgcatggcgg cagtgcgctg gtcgatatga cctactatga aaataccgtg cacgcaatgt
     9541 ggctggcaag ccaggaagcc tgcgataagc taccttccgg gcgtgtgtac aatatcacca
     9601 acggcgagca tcgcacactg cgcagcatcg tgcagaagct gatcgacgag ttgaatattg
     9661 actgtcgtat tcgttccgtc ccctacccga tgctggatat gatcgcccgc agcatggagc
     9721 gtttaggccg caagtcagca aaagagccgc cgctgaccca ctacggcgtc tccaagttga
     9781 attttgactt tacgctggat attacgcgtg cgcaggaaga gttaggttat cagccgatca
     9841 tcaccctaga tgaaggtatc gagaaaactg ccgcctggct acgcgaccac ggaaaactgc
     9901 cgcgctaatc ctgcccgtat ttctccagca atgcttcggc aatcgcctga gtttctgcat
     9961 ccgcttcgcc gttatataac gtcgggcgga aatgcatctg gaatgccata atcacgcgcc
    10021 gctgctcgcg cggtgtcata tcaggtttaa cgtcataacc gtagcgcgcc aaaagctcca
    10081 gcaatgacgc ggtatctacc ggtgtgtgcg gcgcgcgccc ggcaaggtaa aagttaaccc
    10141 gctgcgcatc cggccaggca ccaatcccct gctgcgccag ttgctgccag ggaaataatg
    10201 gccccggatc gtctttgcgc tgcggtgcga tatccgcatg tgccactacg ttttccggct
    10261 tgatgtgata acgggcaata atatctttcg ccagtggaat aagcgcctga atctgtgtcg
    10321 gttcaaacgg ggcaaaatat ttcactccgg ccgatttttg ccagccacgg ttttccagct
    10381 caatgccaat agaggtgtcg ttaaggcgcg ttgccccgcg ccaggcgcta atccccgcat
    10441 gccaggccag ttcttgttcc ggcaccagtt gccagatgcg cggtttaccg ttgtatcgcg
    10501 gtggtaccgc agggaccaga taatgcgagc tgacctgctt atcggtcagt gtcgccagcg
    10561 agctatcaaa atcatctgcg gtgtagtgga tcaccagcac tttaatgcgc ggatacgccg
    10621 cctgcgcctg gcgtcgggta tcaagctgat atccctcttt ctcgacaatg cctttttcgc
    10681 ctgcacaccc tgccaataac agagcggcag cgaccagcca gaagactctt ctcatcgacg
    10741 cgttttcacc gccgtaccgc tgacgctaac catcagcata ctgccgtttt gcccgaccgt
    10801 ttcgtagtcg atatcaatac cgacgacggc atcggccccc agcgcccgcg cctgggagcc
    10861 taattcctca aaggcgatct cccgtgcttt acgcagttct ttttcatacg caccggaacg
    10921 tccgccaacg atatcgcgga taccggcaaa gaaatcacgg aaaatattgg cacctaaaat
    10981 cgcttcgccg gtcaccacac cgcaatattc aacgatggtc tggccttcca gagttggggt
    11041 tgtagaaaat tgcatgatcg atctccttgt ttgttaagcc ttcgatctct aaagcattat
    11101 cgaactgata tgccatgttg ggaagaaggt tttgatcagt ttaggaaatc atctcattgt
    11161 gtcaaaaaca cccacttaag tctttacgat tatccgttgc ataacccgat attggtttca
    11221 ttatagggta ccgcttacct gtcttattat gttgcaccaa caacattaat gagcggaaag
    11281 aggcaactta tattgtaaat gactcctaaa gtcttcccca tcttgtgact gaggtatata
    11341 cctctggatt gctttttggt cagacgccgc aaaacaagaa agtaataaga tctcaatgct
    11401 tttgtcatta atataagcga agatcattct tgcaaattcg taaataaacg cccgttcctg
    11461 agcgcttaat agtttaatgc aatatttatt cttaagagag aaaataattt caaggaaagc
    11521 atccgcagca accttcacgt tattcccggc gctcatatta tatatatccc ttgcacagat
    11581 agagtacaga gatagtaaac agccatacaa ataatcattt attaactctt ttgtccattt
    11641 tactggcaac catggtttta tcttgacacc atgcatacta cagtcatgaa aaagacattt
    11701 gctgaaatta actcttatca tatcaacatt aaaaaaatgg caattaataa attttaaaga
    11761 atgagtattt acgttgttaa ttttaacaga tctaaacgaa acatttttga atacattatt
    11821 tattatattg ctatttgtta ttttaacatc acctaaactg ccgttttcaa aacaacattt
    11881 tttcaatatg ctttcattca caacagtgtg tgataagtag agattactta tgttttgctt
    11941 tattaatgcc acgtcattaa aatgactata tttaatgaaa aaaccatcat tgagtagtgt
    12001 tttaagattg actaaattac ttgcatcttc acgtaccatc gtggcaactg gaacaggcag
    12061 agacatgaaa acttcttcat tcaccatcaa gtgaatcatg cattgcgttc cctttgtcat
    12121 atccacacat acgctacaaa tatcgtggta atgcgaagag cgcatttcag gaaacagatt
    12181 aaacacggta tcttgaaagg cactaaatcg tgttaataaa tcctgataat gaacaggttt
    12241 tgtttcaaaa ttatcatcag caaatccaag cactattgta tgctctacat ctaacgcagt
    12301 ttcaaatggc tcttcttctg taaaatcatt atttaaaacc tcacctgacg tattatcact
    12361 gtcataaaaa gtgttcatca atgcgatatt atttgttaca gcgtcttttt cgatattctc
    12421 actaattgca tatcgcctgc tataatcata aaaatgaata aacgcgccct ttataaagga
    12481 tatatattgt tctttgttaa agtcaatacg gctgtataat ttttcaaaaa tcacattcgc
    12541 aattttacta ccaatggtat gcctgtataa taatgggtgc gattcaatcg taaaatgctt
    12601 gtctaattga tcttgtaatt tattctcata gtccagaggg aaaagaaact gttgtctttc
    12661 aagtttccca ttcgatacat tacgtattcc aatagagatt ctttcattac ccgcctttat
    12721 ttcaacttcg ttatcttctg atttacccag atatttttta acatcagtct cgaaagcatt
    12781 actaaaaaaa gtattaaatt ggtaattcgc tgatggcata ttttccgcga gcaacacatc
    12841 attttttatg agatcactgt cagcatttat tgctaatatc agatgcaagt agatggtatc
    12901 ccatgtagac aactcaccct tacgactgga atttaatatc gcgaaaaatt ccctcagtga
    12961 atccaggaaa ttatcacttt gatttagttg tgtgcctgga aatgatgatg cttgatcaga
    13021 tatattgttt atcatattaa aaatcaccca agaaaaacga taatccaccg aactcctcgt
    13081 gctttataat tataaaagca caacgccttt tatattcata ttaattaacg agatactgaa
    13141 acaacttaat ttattcttcg ctaaaattca ttagcttaaa ttctttaccc acaattgtag
    13201 cataaataat attatcgaga gtcaaagctg ggactatcaa atttcgctca ttttcaaaaa
    13261 tacaacgtaa ttttggcaat catcttaatc gcaggatctc ttatcgcatg tgaaaaattt
    13321 tgtaatgatt agacaataac cgcgttcatt tatcacgcgt tataaatgtg aaactggctt
    13381 cttgcagatg ctcttaacct ctacaggagg atttatacca ccatttcaga actatcatat
    13441 ttaccttaaa tgatcatttc agccatataa cacgctcaac ctcacaaatg gtttacactg
    13501 tctattgttt gggtaattta agtaagtcag ctacgagcaa ccttgagtgg atagccagca
    13561 atggactcaa acccaatcag gtgttcagat tgtttcagaa ttgtattaaa aacaatgtat
    13621 taaataatct gatagcgttt ggtcgtagtg tgacgcagac agcaagctaa taaggaaatg
    13681 attatgcgct accgttcact atctctactt attccatgtg cactgctgct gagtgcctgt
    13741 accaccgtca cgccagctta taaagataac ggtccccgca ccggttcctg tgtccagggg
    13801 gggcctgaca gcgtggctca acaattctat gactaccgca ttcaacatcg cagcaatgac
    13861 attaccgccc tgcgccccta tttgagtgac aaactggcaa cccttcttag cgatgccagc
    13921 cgcgataata gtcatcgtga attgttaagc agcgatccgt tctccagtcg aaccaccttg
    13981 ccagatagcg cccacgttgc cagtgcatcg actatcccta atcgtgatgc ccgtaacatt
    14041 ccattgcgcg tagatctcaa acagggtgac cagggttggc aggatgaagt gttaatgatt
    14101 caggaaggcc agtgttgggt catcgacgat gtgcgctact taggcggcag cgttcatgcc
    14161 acggcgggta cgctccgtca gtccatcgag aatcgctaac gccttgatca accaataaac
    14221 cccgtaaatt gtccggcatt tcgtgaggaa tgccgacatt tatgctcgcc gaccaccgcc
    14281 cccgttattt tgtgctatgt ttattgaata atgcgctttg cttttaactt ttaaagcaga
    14341 aatattgcat aattattctg tcaaaggtac tatcttcgac ctcaatagct atcagactgc
    14401 cagtatacga gtgtcaatga gtattcaatt aaacggcatt aattgcttct acggcgcgca
    14461 tcaggcgctg ttcgatatca cgctggattg cccacagggc gaaacgctgg tgttacttgg
    14521 ccccagcggc gcgggtaaaa gctcgctgct gcgtgtactc aatctgcttg agatgccacg
    14581 ctccggtacg ctcaacattg caggcaacca tttcgatttc accaaaacac cctctgacaa
    14641 agcgattcgc gatttgcgtc gtaacgttgg catggtgttt cagcaataca acctgtggcc
    14701 gcatctgacc gtgcagcaaa acctgattga agcgccctgc cgtgtactgg ggttgagtaa
    14761 agatcaggcg ctggcccgtg cagaaaaact gctggaacgt ctgcgtctca aaccttatag
    14821 cgatcgttac ccgctgcatc tttctggtgg tcagcagcag cgtgttgcta ttgcccgtgc
    14881 gttgatgatg gaaccgcagg tactgctgtt cgatgaaccg accgccgcac tggacccgga
    14941 aattacggca caaatcgtca gcatcattcg tgagctggca gaaacgaata ttacccaggt
    15001 gatcgtcacc cacgaagttg aagtggcgcg taaaaccgcc agccgagtgg tgtatatgga
    15061 aaatggtcat atcgtagaac aaggcgacgc gagctgcttt accgagccgc aaaccgaagc
    15121 atttaaaaac tatctctctc actaagattc gggataacaa caatgaaaaa agttctgatt
    15181 gccgcgttaa ttgcaggttt tagtctttcc gccacagctg ccgaaaccat tcgttttgct
    15241 accgaagcat cctatcctcc gtttgaatcg attgatgcaa acaaccagat cgttggtttt
    15301 gacgtcgacc tggcacaagc gctgtgtaaa gagattgatg caacctgcac tttctctaac
    15361 caggcgtttg acagcctgat cccaagcctg aaattccgtc gcgtagaagc cgtgatggcg
    15421 ggcatggata tcactccgga gcgtgaaaag caggtgctgt ttaccacccc gtactatgac
    15481 aactctgccc tgtttgtggg tcagcaaggc aaatacacca gtgttgatca gctgaaaggc
    15541 aaaaaagtcg gcgtacagaa cgggacgaca caccagaaat tcattatgga taagcacccg
    15601 gaaatcacta ccgttccgta tgacagctac cagaacgcaa aactggatct gcaaaacggg
    15661 cgtatcgacg gcgtcttcgg tgacaccgca gtggtcactg agtggctgaa agataacccg
    15721 aaactggcgg cggtgggcga caaagtgacc gataaagatt acttcggcac tggcctcggc
    15781 atcgcggtac gtcagggcaa cactgagctg cagcagaaac tcaacactgc gctggaaaaa
    15841 gtgaagaaag atggcactta cgaaaccatc tacaacaaat ggttccagaa gtaattcctg
    15901 atgaatgaat tttttccttt agcaagcgcc gccgggatga ccgtcggcct tgccgtttgt
    15961 gcattgattg tcgggctggc gctggcgatg ttctttgcgg tatgggagtc ggcaaaatgg
    16021 cgtcctgtcg cgtgggcagg ttcagcgctg gtaaccattc tgcgtggcct gccagaaatt
    16081 ctggtggtgc tgtttatcta ttttggctcc tcgcagctgc tgctgacgct ttcggatggc
    16141 ttcactatca atcttgggtt cgtgcagatc ccagtgcaga tggacattga gaacttcgac
    16201 gttagcccgt tcctttgtgg tgtcatcgct ctgtcactgc tgtatgccgc ctatgcctcg
    16261 caaacgcttc ggggcgcgtt gaaagcggtg ccggtgggtc agtgggagtc tggtcaggcg
    16321 ctggggctgt cgaaatcggc tatctttttc cgtctggtga tgccgcagat gtggcgtcat
    16381 gcgctgcctg gcctcggtaa ccagtggctg gtgctgctga aagataccgc gctggtcagt
    16441 ttgattagtg tgaatgattt aatgctgcaa acaaaaagca tcgctactcg tacccaggaa
    16501 ccatttacct ggtacattgt ggcggcggcg atttacctgg tgatcaccct gctcagtcag
    16561 tacattctca aacgcattga cctgcgcgcg acacgttttg agcggaggcc cagctaatgt
    16621 ttgagtattt acccgaactg atgaaagggc tacacaccag cctgacgcta accgttgcct
    16681 cgctgattgt ggcactgatt ctggcattga tttttaccat catcctgacg ctgaaaacgc
    16741 cggtgctggt gtggctggtg cggggttata tcacgctgtt taccggtacg ccgctgctgg
    16801 tgcagatctt cctgatttat tacgggccgg gccagtttcc gacgttgcag gagtatccgg
    16861 cactgtggca tttgttgtca gaaccgtggt tatgtgcgct gattgcgtta tcgctgaaca
    16921 gtgcggcgta taccacgcag ctgttttacg gtgcaattcg tgcgatccca gaaggtcagt
    16981 ggcagtcctg tagcgccctg ggaatgagca aaaaagatac gctggcgctc ctgctgccgt
    17041 atgcctttaa acgctcgctc tcttcttatt ccaacgaagt ggtgctggtg ttcaaaagta
    17101 cctctctggc ttacaccatt acgctgatgg aagtgatggg atacagccag ttgttgtacg
    17161 gacgcaccta cgatgtaatg gtgttcggtg cggcagggat tatttacctg gtcgtcaacg
    17221 gcctgctgac gctgatgatg cgtctgatcg agcgcaaagc actggcattc gaacggcgga
    17281 attaatgtag ctgcgtacat tcgccggggg ggtttgtagg tctgataaga cgcgccagcg
    17341 tcgcatcaga catcagcacg gtgcctgtgc cggatgcggc gtggacgcct tatccggcct
    17401 acatagtgca taaattctta taatgaagac ggacaactca ctaagttatc cgtctttttt
    17461 tatttcattt aaattattta atcatattta ttgcatataa attcacttga tggcattgtt
    17521 atcccatgcc gcagacacgg ccaaaaatca taagattgac agacgggagt tccatcatga
    17581 aaaagttagt tcttgccgct ttacttgctt cctttacttt cggtgcttct gccgcagaga
    17641 aaatcaattt tggcgtttca gccacctatc caccctttga atctataggt gctaataatg
    17701 agattgtcgg ctttgatatc gatctggcaa aagccttgtg caaacaaatg caggcagaat
    17761 gtacttttac taatcacgcg ttcgacagcc tgatcccgtc actgaaattc agaaaatatg
    17821 actccgtaat ctccggtatg gatatcacgc ccgagcgtag caagcaggta tcgtttacca
    17881 cgccctacta tgaaaactca gccgtcgtga ttgccaaaaa agatacctac aaaacgtttg
    17941 ccgatctgaa aggcaaacgt attgggatgg aaaacggcac cacgcaccag aaatatattc
    18001 aggatcagca ccccgaagtg aaaactgtct cctatgacag ttatcagaat gcctttatcg
    18061 atctgaaaaa tggtcgtatt gatggggtat ttggtgacac agcggtggta aacgaatggc
    18121 tgaaaaccaa tccgcaactg ggcgttgcta ctgaaaaagt gaccgatccg caatacttcg
    18181 gcaccggtct gggcatcgct gtacgtccgg ataacaaagc tctgctggaa aaactgaata
    18241 acgcgctggc agcaattaaa gctgacggta catatcagaa aatcaatgac cagtggttcc
    18301 cgcagtaatt gagactgatg acaaaagtaa gattgcctga tgcgctgcgc ttatctggcc
    18361 cacatcaact ctgcaatgta ttgatttagc atgattttgt aggccggata aggcgtacac
    18421 gccgcatccg gcatgaacaa agcgcacctg atcagcaatc tgaagccgca cctgcggctt
    18481 ttttattgct tcaccagcag cgtcatcact tcatagtgcg cggtgtgcgg gaacatatca
    18541 aaaagctgta cccgttcgat gcggtaacct ggcagttcgc ggatatcttt cgccatggtt
    18601 tgggcgttac agctggagta gataataaaa cgcggtgcca tcgttgagag ataatcacac
    18661 agcggtttac caatgccgcg gcgcggcggg ttaaccagca ccagctccgg cacatccccc
    18721 tgagcggtgg caaactgagt ggagtccagc gcctgaaatt gcaaacgcgt taagcccagt
    18781 tcagcggctg actgctttgc acaggcaatg gcctctgatg caatttcgat cccggttaac
    18841 tgcatgtcag gcgtcgcgca gtgtaaacca aagccaccca caccgcagaa cagatcccac
    18901 atatgtttaa ctggcagctg ccgtacccag tcgcgcgcgg tagcgtacag ctggctggcg
    18961 accgccggat tagtctggaa gaaactttgc ggacggatcc acagcggtac gtcattaaaa
    19021 cgctctgcca gcgcctgttg ttcagtcagg tagatctccg tctccccttc cataatcgcc
    19081 atatgtaccg gctgaatatt gacggtaata actttcagtt gcggtagttg ttcctgtaac
    19141 cacggcagcg ccttacgcag ttgtgccagt ttggtttcag aacgcagtac aaagcgcagc
    19201 atcatgccgc catcgctctg gctttcagtc agcagaatgt atttcagttc gccacgctta
    19261 cgcgccacgt tgtagggcgt taaccccgca cgggcgataa acggttttag cgccgcaaaa
    19321 acgggcgcaa aagaggcagg ataaagcggg cagtcacaaa ggtcttccgg cgtgccatcg
    19381 cgatgcagca taccgagcag tggtttttca acgctaccac tcaccaccat tttggcttta
    19441 ttacgaaacc cttgttccgg gcctgacacc ggcgcgcacc attcctcaac cggaaaatcg
    19501 gcgagcagat ttttaagatc ggcggtttta gcggagagtt gctctggaat cggctgcgtt
    19561 atccactgac aggaacgaca gcgacccgcg tcgtaaagtg cgcactgcat acattgacct
    19621 tcacatcatc aggggcgacg attatacaca ttattgcaac tggaagaacc gccgactggt
    19681 cgagggaacg aacagcagca tcaggatgag catatccggc agcttctgca gcatcaggct
    19741 atggaagatt tcacgttttg attcaccggg aatgctgaac agctccggat aaccgtaccc
    19801 cagcgaagcc gcccacaagt aacttgcggc ggtgatttgt gtcagcagat atagccagcg
    19861 cgcccagcga cgccctttca ccagtgaaaa cgcacaccag atctcaatga aaaccagcac
    19921 cagactgctt aaaaagacca gcgttaaact ccaggtttgt acgctgcgat gaatgaactc
    19981 gccaataccg cgcacgccca gcgtattgaa aatcatcagc acgtcgaggc cacggatcat
    20041 aataatggcg agcgccgcca cctgcaccag cgcaggcaca ttcaggcgag catgagatga
    20101 agatgttttc ttaaaaaatc ccaacgtttc gtcttccatg aaaacaatgc cgcggcatgc
    20161 gcggcattat gtagccaggt tggcaaattt tagtgtcttc agccatgtct tgcacgctgg
    20221 atatcgcgta tccgctgttt ttctgcgcga gccatcagat accaggcgat aaatccgaca
    20281 attccgaccg caccgagaat taacgttgca agggcgttga tttccggatt cactcccatc
    20341 cgcacgctgg aaaagaccag catcggtaac gtggtggctc ccggcccgga aacaaagctg
    20401 gcgatcacca gatcatcaag cgacaaagta aaagccagta accagccaga aatgatcgcg
    20461 ggcatgatca tcggtagcgt aatgacgaaa aacactttca gcggcgtcgc accgagatcc
    20521 atcgctgctt cttctatcga gcgatccagt tcccgcaatc ttgaagaaat aacgaccgcc
    20581 acataagccg tacaaaaagt gacatgcgcc agccagatgg tgagcatacc gcggtccgca
    20641 ggccagccaa tggcatgagc aagcgcgacg aataataaca acagcgacaa gcccgtgatg
    20701 acatctggca tcaccagcgg cgcggtgatc ataaaggcaa aaccatttga tccgcgaaac
    20761 ctgccaaaac gcaccagcac caccgccgca atagtcccga ggatcgccgc cgccgttgcc
    20821 gcacaggcag caattgtcag gcttaaacca accgcactca tcatcgcgtc atcgcgcaat
    20881 aactcaccat accagcgcgt tgaccagccg gcccacaccg tcaccagctt cgagctgtta
    20941 aacgaataga taaccagcat cagcattggc gcgtagagaa aggtaaagcc cagcagcaaa
    21001 atcacaatcc gccagggcga acgaactacc ggtaaattat tcatccgtgt tctcccacgc
    21061 ttttttgctg gtgtttgtgg aaccacatta tcggcacaat tagcagcagc aacatgatga
    21121 tcgctaccgc cgaggccacc ggccagtcgc ggttattgaa aaactcttgc cataacacgc
    21181 gcccgatcat gatgctgtcc gggccaccga gcagttccgg gatcacaaac tcgcccacag
    21241 ccgggataaa caccagcatc gatccggcaa taatcccacc tttagtcagc ggcacgatca
    21301 cagtaaagaa cgttttcagc ggtcgtgcac cgagatccag cgctgcttcc accagcgaat
    21361 aatcaatacg aatcaacgcg gtataaatcg gcagtaccat aaacggcacg taagcgtaaa
    21421 caatgccgat ataaacggcc agattggtat gcagaatggt cagcggttga tcgataaccc
    21481 ccagccacag cagaaaatta ttcagcacac cgttgttttt taatattccc atccaggcat
    21541 aaacgcggat cagaaacgag gtccacgacg gcaggatcac cagtagtaat aaaatattac
    21601 gggtcgaagg cttactgtgc gccaccgccc acgccagcgg atagccgatc agtaaacagc
    21661 aaaatgtcga aatcgccgcc acctgtaacg actggagata agcatcaaaa tagagcggat
    21721 cgtcggtcag ttgcagaaaa ttaccgagat taagagtgat ggaaagttgc ccgtcagccc
    21781 actccatcag ttcggtataa ggtggaatag cgcgcgccat ctctcccagg cttattttaa
    21841 agacaatcag aaatggcagc agaaacagca agatcaacca gatatatagc aacgcaatga
    21901 ccagtttgcg cccatgcttc atttgcagct gcgacaacca cagcttaaat ccgcccggcg
    21961 gtttcgactg ggcagcaggt tcaagtgtac tcatctttgc tccttaaacc gtcagcacca
    22021 cacagctgtc cacttcccag cacaaacgca cttcgtcgcc ccaggtcggt aaccctttac
    22081 gatgacgatg ggcgttttgt agctgggcgc tgatcatctg cccacttttc agtcgaacgt
    22141 gatacaccga aagatcgccg agataggcaa tgtgtatcac ctcccccacc gcgaagttac
    22201 aaccattggc gggcggctct tcgcaaagca tgattttttc cgggcgcagc gctacgtgta
    22261 ccggcacgtt atcgaccact gaagcatccg catcgacttt cagtggatgc accagccccg
    22321 gcgaatcaag caccaggcca tcttcctgac gctctttgag tacaccttca aagacattaa
    22381 ccgaaccgat aaattcagcg ctgtagcggg tagttggatg ctcgtagatc tcttccggct
    22441 cgccaatctg gacaaatttc ccgcgattca taatggcgat acgccccgcc atggtcatcg
    22501 cctcttcctg atcgtgggtg accatcacac aagtcacacc gacgcgctcc agaatatcca
    22561 ccacttcaag ctgcatccgg tcacgcagct ttttatccag cgcgcccatc ggctcatcga
    22621 gcagtaatag tttcgggcgt ttggcaaggc ttcgggccag ggccacacgc tgtcgctgac
    22681 caccggaaag ctgatgcggt ttgcgtttgg cgaactcctg catgtgcacc aggccgagca
    22741 tctcatggac ccgactggca atttccgctt tcggtagttt gtcctgtttc aggccaaaag
    22801 cgatgttctg ttccacggtc atatgcggaa acagcgcgta agactgaaac atcatattga
    22861 tggggcgcag gtaaggcggc acctgtgaca aatcgacgcc atcgagcatt atctgcccgg
    22921 cagaaggttg ttcaaaacct gccagcatac gcagcagcgt ggacttgcca cagccggatg
    22981 cgcccagcag cgcgaagatt tcacctttgt agatggtcag gctgacatca tcgaccgcat
    23041 gctgaccatc gtaggatttg gtgaggttgc ggatttctaa tagcggcgtc agcgccttac
    23101 gggttttcgc ctgcgggcga gggatagcgt cattcacggc tgctctccgg cattgaatgt
    23161 gtgcaagcgc accatgatgg tgcgaattgc cggctggtgt ggtgcgcccg cccggcatct
    23221 aaggctgcgg attattttcc gctcttcact ttggtccacg cgcgggtgcg cacacggtcg
    23281 attttcggat cctgtacttt cagagtgaat agcttcgcac gaacgtccgc cggaggataa
    23341 atacccgggt tctcacggac ttccgcactc accagcggcg tggctgcttt attggcgttg
    23401 gcatagaaca catggtcgga aatatgcgct actacatccg ggcgcagcag gtaattcagg
    23461 aactgatagg cttcgtcttt gtttttggca tccgcaggca tggcgaatac atcaaagaac
    23521 gccatcgccc cttcttttgg aatcgagaac gagacattca ctccattctt cgcttccttc
    23581 gcgcggtttg acgcctgcca gacatcacct gcccagccga tagcgacgca aatatcgccg
    23641 tttgccaggt cgttaatgta ttgagatgaa tggaaataac gaatgttcgg gcgcagcttt
    23701 aacagcagat cggttgccgg tccggtgtaa tcatccgctt tagtgctgtt gggatctttg
    23761 ccgagataat tcaacacggt agcaaaaact tcttctggcg catccaggaa agagacaccg
    23821 cagcttttca gtttttccag attttcaggt ttgaggatca agtcccagct atcgacgggc
    23881 gcgttttcgc ccagcacagc tttaacttta tcaacgttat agccaatccc ggtcgtcgcc
    23941 cacatatagg gcatagcaaa tttattgtcg ggatcgtgtt tggcgaccag cttcagcagc
    24001 tccgggtcga gattcttcca ctccggcaat ttgcttttgt ccagcggctg gaaaactccc
    24061 gcagtcaact ggcgttcaag aaagctggca gatggaacca ccaagtcaaa gccggtgcta
    24121 cccgccatta atttgccttc cagtacttcg ttagagtcga aaacatcgta gacgacttta
    24181 ataccggttt ctttttcaaa attggccacc gtgtccgggg cgatataatc agaccagtta
    24241 taaatgtgga gtgttttttg ttcagccgcg agcgtgccga cagagacggc catcagagca
    24301 cccgcagcca gacccgaaag ccatttttta tttaaggcgg tcatatctct ttccttctga
    24361 aagttcgtta acaaacgaac aaaagttgtc acactaaaag atatgcaata aacgtgcata
    24421 ttttttatcg gcggggtgta agaaaagaga actctctccc ggccagcact gctcgctata
    24481 taaaagcatc gcaagaataa ctttagccag ggtttgagac tatagctcgg attaaaaaac
    24541 ggtatttaac aaatttttat gcgtatttgc tctatgtgat aaagccaaat cgacacaaga
    24601 gaagtgaaat aatctttaag aaaaggttaa aaacagcaga taattttgca gcgcgcagcc
    24661 actgcaccag cgactgcgtg ctcgaaagga gttatcagtg gaggaaatag ttttcggtaa
    24721 cgttgttttg cccttcatcg tcagtcggta acagcagttg atgggcatta gcttcaagaa
    24781 tgaccatcga aatctgctct tcgctctgac gcacgaacca tgcaaactgc tcataggtta
    24841 cgccctgcat aacggataaa gactggcaaa ccaccagctt tggcagatta tcatcctgca
    24901 tatcaagaaa tgctttcacg gtcagcgaac tggcattgat ggcagataaa tccgccgcca
    24961 gcggcaatac ggctgaaggt cggacttccg ccatggcaga aaacaggatc gtgttatcga
    25021 tcagatcgat tttggcatca aagacaccgt cgaaattctg catatggggc agatgcagag
    25081 cctgacagtt atcacattca aaaaaactca tccccaggtc atcaagccat tgacgcagcg
    25141 tatccagacc aggaacgacc agcgatgtca taattttgta caacctcttt cgataaaaag
    25201 accggcacag cttacgcaaa aagcgcaggc aaaaccatga tcagtaatgt gattgcgact
    25261 aaccacccgt tttcaggcaa tattctgtcg tagcgtggcg ttcgatccag cggatcattt
    25321 tacccgcgat gtcgatacca gtggtttttt ctattccttc cagccccggc gacgcattca
    25381 cttccatcac caacggcccg cgattagcac gcagaatatc cacaccagca acgtccagcg
    25441 ccatcgttcg cgcggctttt atcgcgattt cacgctcctg tggtgtgata ctggcgacgc
    25501 ttgccgcgcc gccacgatgc aaattggaac gaaaatcgcc ctctttcgcc cgccgttcaa
    25561 tcgcagcgac cacttcatcg ccaacaacca gacagcggat atcgcaccct tgcgcctctt
    25621 tgatatattc ctgcaccaga atatgcgcgt tcagaccgcg gaaagcgtca atcacgcttt
    25681 ccgccgcctg acgcgtctcc gccagcacga cgccaattcc ctgcgtgcct tcaaccaact
    25741 tgaccaccag cggcgcacca ccgaccatgt cgattaaatc gctggtatca tccggcgaat
    25801 gcgcaatgcc cgtgacaggc aggtcgatgc cctgacgcgc cagcagttgc atggaacgca
    25861 atttgtcacg cgcccgggca atggcgaccg actcattgag cggatagctc cccagcatct
    25921 cgaactggcg cagtgccgcc gtcccataaa aggtaatggc ggtgccaata cgcgggatca
    25981 ctgcgtcaaa atggggtaac ttgcggcctt tgtagtgaat agaagacgcc gcaggattta
    26041 tgttcatgta gcaagaaagc ggatcaagaa tttcaaccag gtgaccgcgc tgtatagcgg
    26101 cttcacgcag ccgcttacac gaatagagcg ttccatcccg ggacaatatg gcaattttca
    26161 ccctgcacct ctctgtcaga cctggtcaaa agcccgcgta tcatacatcg acgtggcggt
    26221 tttagcgcgt cgcccaaccc tgtttgtgca aataatccag aataaatggg cggctttctt
    26281 taatgattgt tcggcggata tgatcgctcc aggtatcccg gcgattattg ctgccacggg
    26341 tgaggtaata ttccgccagt tgctcgtcat actgcgccag tgcgtcttta tccagcggtt
    26401 gatagctgtt ttcatgcacc aaaatggagg ccggtaaacg cggcttgaga tccggattat
    26461 ccgcaggcca gccaaggcac agcccaaaca gcggcagaac atgctgcggt aatttcagca
    26521 gtttcgtcac cgcttcaata ttattgcgca ggccgccgat atataccccg cccaatccca
    26581 gcgattccgc tgcggttaat gcattctgcg ccatcattgc cgtatcaacg acaccgagca
    26641 acagttgttc cgccaggccg agctgagcat ccggacagat ctgtaaatgg cggttaaagt
    26701 cggcacagaa cacccagaac tccgccgctt gcgctacgtg tttttgcccg ccggtcagcg
    26761 tcaccagttc ttcacgtaat gctttgtcgg taatgcgaat aatgctacta cactgcaaaa
    26821 aactggaact ggacgtcgca cgggcgctgt taataatcgc ctcacactgc gcttcggaaa
    26881 tgggttcatc agtgaaatgg cgaatggagc gatggccaca aataagttca atggttggcg
    26941 tcattatctt tttctctttc tgaacgtgaa tattgcggtg gacggttcat cagctgtggg
    27001 gcaagacgtt ttgccacctg aagaataacc accaccgcag cgggaagcat gagcaaaaca
    27061 ccgagaaaaa tcatcagaat ctgcacttct ggccgagaaa atggctcagg cagcgacagg
    27121 gagtcgctta ccgacagcag cgccaccgcc agtagcatca ttccgataaa ttccagtatc
    27181 aacacgcctt taggcaattt accgatcgcg cgcatacgct tccctctgca aagtgagcct
    27241 tcagtctaaa acttttcact gtattgtgtt taacagttat agcttttagc aattaatgca
    27301 acaggttaaa cctactttca gcgaatacat tttagcgtga tcattacagg cataaatcta
    27361 tgaggagaga aataatgcaa accgttattt ttggtcgttc gggttgccct tactgtgtgc
    27421 gtgcaaaaga tctggctgag aaattgagca atgaacgcga tgattttcag tatcagtatg
    27481 tagatattcg tgcggaaggg atcactaaag aagatctaca acaaaaggca ggtaaacccg
    27541 tagaaaccgt gccgcagatt tttgtcgatc agcaacatat cggcggctat accgattttg
    27601 ctgcatgggt gaaagaaaat ctggacgcct gatcgtctga caagccctcg cgttgagggc
    27661 tttactgatt ttttctgtgc tgtggtttaa acaaactact gataaataag aaacacagtg
    27721 cccccagcgc acaccagaac accgcgctta gtaaccatgc cagctcttgc cagaatgagc
    27781 gcgtcggtga aaaaaacagc cgcataatga gcattgaaca gggtgccgcc agcattgcgc
    27841 caaatagagg tttcaggact tctctacgct gtgaaaagaa actggcaact gctccaggaa
    27901 gaatgaaaaa cagcaagccg atttcaggat gcccggcagc ccgaaaagcg cctttcatgt
    27961 gcgtcgccag aaaaaggcac accacaatga agaggacaaa acagcagatt gcccccgccc
    28021 aacgttgttt atgtttcact cgttcctcct gacactgcgt ctatcgaaca catttttcgc
    28081 cagtgtggcg ttcagtaaga taaagccgct tcgcattcca tgctaatata ggccaacgca
    28141 attcatatag ccgttgatac ctaatgtgat tacactagta aaatatattg ttactttact
    28201 atcgtttagg tgcgctgaat gaatctgcgc cctgaattct ggtaaaaaac attatcgtaa
    28261 attaccattt ctttcaacag cttactagta aacaagaagt tagcctccgt gaatataaac
    28321 gtcgccgaat tgttaaatgg gaattacatt ctgttattat ttgtggtcct cgcgcttggg
    28381 ctatgtctcg gaaagttacg acttggttcg atccaactgg gtaattccat tggcgtttta
    28441 gtcgtatcgc tgttattagg ccaacaacat ttcagcatta acaccgatgc gcttaatctt
    28501 ggctttatgc tgtttatttt ctgcgtcggg gtcgaagccg gaccgaactt tttttccatt
    28561 ttttttcgcg atgggaaaaa ttacctaatg ttagcactgg tgatggttgg cagtgcgctg
    28621 gtgatcgcct tagggttagg taagctgttt ggctgggata ttggcctgac ggccggtatg
    28681 ttagcaggct ctatgacgtc gacaccggtt ctggtcggtg ctggcgatac actgcgtcat
    28741 tccggcatgg aaagcaggca gctctcactg gcactggata atctgagcct cgggtatgcc
    28801 ttaacctatt taatcggtct ggtgagtttg attgttggtg cgcgttactt gccgaaattg
    28861 cagcatcagg acttacagac cagcgcccag caaatcgccc gcgaacgtgg cctggacact
    28921 gatgccaacc gtaaggttta tttaccggtg atccgcgcct atcgcgtcgg cccggagctg
    28981 gtggcctgga ccgacggcaa aaatctgcgt gaactgggta tttatcgaca aaccggctgc
    29041 tacattgaac gtattcgacg taacgggatt ctggcaaatc cagacggtga tgccgtgcta
    29101 caaatgggcg atgaaatagc gttggtaggc tatcccgacg cccatgcccg actcgatccc
    29161 agcttccgta acggtaaaga agttttcgat cgtgaccttc tcgacatgcg tatcgtcact
    29221 gaagaagtgg tcgttaaaaa ccataacgct gtaggtaaac gtctcgcaca actgaagttg
    29281 accgatcacg gttgcttcct taaccgcgtc attcgtagcc agattgagat gccgatagat
    29341 gacaacgtcg tgcttaacaa aggtgacgtt ttacaagtca gcggtgatgc ccgtcgcgta
    29401 aaaaccatcg ccgatcgcat cggctttatc tcgattcaca gccaggtcac tgacctgctg
    29461 gcattttgcg ccttctttgt tattgggctg atgatcggga tgatcacctt ccagttcagc
    29521 acattcagtt tcggcatggg gaacgctgcc gggttgttat tcgccggaat tatgctgggc
    29581 tttatgcgtg ctaaccaccc gaccttcggt tacattccgc aaggtgcatt aagcatggtg
    29641 aaagagttcg gcttgatggt gtttatggca ggcgttggtc tgagcgccgg tagcggtatt
    29701 aataacggcc tgggcgcgat tggcggtcag atgttgattg ccggattaat tgtcagtctt
    29761 gtgcccgtgg ttatctgttt cttgttcggt gcttatgtat tgcgaatgaa ccgcgcactg
    29821 ttgttcggcg caatgatggg cgcacgcacc tgcgcgccgg caatggagat catcagtgat
    29881 acagctcgca gtaacatccc tgcgctgggc tatgcgggca cctacgcaat cgccaacgtc
    29941 ctgctgacgc tggcagggac aatcatcgtc atggtatggc caggattagg ataaaactga
    30001 agttgccctg aaaatgaaat ttttttgcac aaccgcagaa cttttccgca gggcatcagt
    30061 cttaattagt gccactgctt ttctttgatg tccccatttt gtggagccca tcaaccccgc
    30121 catttcggtt caaggttgat gggttttttg ttatctaaaa tttatctact accctgcaac
    30181 cctctcaacc atcctcaaaa tctcctcgcg cgatagcggc ttacggtcgg tgacaaaatg
    30241 cagcgtcatc ccctcaataa acgcatcaag cgcgcgggcg gttccgggtt caaaccattg
    30301 ttcgagcgtt tgctgactgc gctgcatcca gttttgcatt accgttttta atagcggttt
    30361 tcggctagcc agcgcgtaga gctggtacat cagctccata ttatccggcg ttgcaacctg
    30421 tgagctgtag atcatatcgg tgatagcctg gcatgcgcct tgagcatcac taacatcgct
    30481 aaaaaatgcc tgatattgcc gggacataat ctcagtaaaa ctgctgaacg cctccagcaa
    30541 caactcatca attcctgaaa aatagtaggt catcgatccc aacggtaccc cggcaagggt
    30601 agcaattttg cggtgcgtaa cagcatgtat tccgtaaagt ttcaccgcct ccagcgtggc
    30661 ctggataatt ttttctcgcc gttgcggatc gttagcgcga cgcatgtcat ttcctctgtc
    30721 tgcaattgtg tacaaatgta cacaaccttg ctaatgttgt cttccctctt cttattttga
    30781 cgtgatctat gaccgtaaat tcttcacgta atgcattgaa acgccgaacc tgggcgctgt
    30841 ttatgttctt ctttttgcca ggcctgttaa tggcgtcctg ggcaacccgt acgcctgcta
    30901 tccgcgatat tctctctgtc tcgatcgctg aaatgggcgg tgttctcttt ggtctgtcga
    30961 tcggttcgat gagcggtatt ctctgctcgg cgtggttagt gaaacgcttt gggacacgta
    31021 atgtcatcct ggtcacgatg tcctgcgcat tgatcgggat gatgatatta agtctggcac
    31081 tctggctgac atcgcccctg ctctttgccg ttggtctcgg cgtctttggg gcaagttttg
    31141 gttctgcgga agtggcgata aacgttgaag gtgccgccgt tgagcgagaa atgaataaaa
    31201 cggttttgcc gatgatgcac ggtttttata gcctaggcac gctggcaggc gctggtgtcg
    31261 ggatggcact gacggccttt ggcgttccgg caacggtgca cattttattg gcggcgctgg
    31321 taggtatcgc gcctatttat atcgccattc aggcaatccc tgacggtacg ggcaaaaatg
    31381 ctgccgatgg cacccagcat ggcgaaaaag gcattccttt ttatcgcgat atccagttgc
    31441 tgttgattgg tgttgtagtg ctggcgatgg cctttgccga aggttctgcc aacgactggt
    31501 tacccttatt aatggttgac ggtcacggtt ttagccctac ttccggctcg ctgatttatg
    31561 ccggttttac cctagggatg accgttggac gcttcactgg cggttggttc atcgaccgtt
    31621 acagtcgcgt tgccgtggtt cgggccagtg cactaatggg ggcgttgggt attgggctga
    31681 ttatttttgt cgatagcgcc tgggtcgctg gggtgtctgt tgtactctgg ggactgggtg
    31741 cctcgctggg cttcccgctg accatttctg ccgccagcga taccggcccc gatgcaccaa
    31801 cccgcgtcag tgtggtagca acgaccggtt atctggcttt cctcgtcggg ccgccgctgc
    31861 tgggctatct cggcgaacat tatggattac gtagtgcaat gctggttgta ctggcgctgg
    31921 ttattctcgc ggctattgtc gcgaaagccg tcgccaaacc cgataccaaa acgcggacgg
    31981 cgatggagaa tagttgatga gcattaaatt aattgcggta gacatggatg gtactttctt
    32041 aagcgatcaa aaaacctata accgtgagcg gtttatggct cagtatcagc aaatgaaagc
    32101 acaaggcatt cgctttgtgg tcgccagcgg gaatcaatat tatcagttgc tctctttctt
    32161 ccctgaaatt gctaatgaaa ttgcctttgt ggctgaaaac ggcggctggg tagtgagtga
    32221 aggcaaagat gtttttaatg gcgagctgtc gaaggatgcc tttactactg tcgtggaaca
    32281 tttgctgacg cgcccggaag tggaaattat tgcctgcgga aaaaatagcg cctatacgct
    32341 caaaaaatat gacgatgcca tgaaaacggt ggcagaaatg tattatcacc gtctggaata
    32401 cgtcgataac tttgacaact tagaagatat tttctttaag tttggcctga atctttccga
    32461 tgaactgatt ccacaagtac aaaaagcatt acatgaggcc atcggcgata ttatggtgcc
    32521 agtccacacc ggcaacggca gcatcgatct gattatcccc ggcgtacata aagccaatgg
    32581 ccttcgccaa ctgcagaaat tatggggaat agacgacagc gaagtggtgg tctttggcga
    32641 tggcggtaac gatattgaga tgctgcgtca ggcaggcttt agttttgcaa tggaaaatgc
    32701 cggcagcgcg gtcgtcgcag cggcaaaata ccgggcaggc tccaataacc gtgaaggcgt
    32761 actggatgtg atcgataaag tgcttaaaca cgaagcgcca tttgaccaat aaaaacggca
    32821 gcacgcccgg caacacaata cctgaatgaa ggctgcataa ttcgcggaaa ttaacccgct
    32881 gcattatttc aggcgaaatg attatgaaaa attgtctact gttgggcgcg cttttaatgg
    32941 gctttactgg tgtggcgatg gcgcaaagtg tcaccggcga tgtgccgagt ggatacaaag
    33001 tggttgtagt tcctgattca gtcagcgttc cacaagcggt cagcgtcgcg actgtgccgc
    33061 aaaccgtcta tgtggctcct gcgccagctc cggcttaccg cccgcatcct tatgctcgcc
    33121 atcttgccag cgtaggtgaa gggatggtta tcgaacacca gattgacgac catcaccatt
    33181 aattcgagaa tgcctgatcg cacaaagcag tcaggcattt ttttaccctt cgtgagaatt
    33241 tcccatctgt ttatctttta aaaagataac catcagcgac agccacaaaa ttccgttgac
    33301 aaggttgaag agattaaact gtccgttgcc cccgttcagc caggcatgtt tgctgatttc
    33361 aataccaacg gtaaagatca gcatttgcag cattcccatc gccgcagaaa ccgtaccttt
    33421 actcatatcg ctggcaaaca gggttaatcg caccagtccc gcattcgcca gaccaatacc
    33481 gaaagcataa atacttaacc cggcagtcat ccataaatac gcgtgcgatg agataaccgt
    33541 tgccgcagca gcgaccaaca gaccaatcat aatcggccag ccgcccataa ttatcacgta
    33601 cggtgcggcg cgaggtcaga cgcgctaaca gcaagttacc cgcaattaac gccccgaaaa
    33661 taggcacttg cagcaagcca tattcatagc tgctcaactg ctccccggta atgatgataa
    33721 tcggcgactg ggcgatccac gccagcaatg gcaggctaac gaatcccagc gccagcgccc
    33781 ccgccacaaa gcggccgttc ttcagcacca gcttatagtc acgaccgagt tctttcagtg
    33841 acagtttctc gcctatacgc gtggcggttt caggcatagc tcgttgcaga ccgaaaaagg
    33901 agatcgctgc caatgcggca aacaaaacaa acatcccctc ccagggcagc acatggatcc
    33961 acgccgcgcc caccagcgga ccaagtagcg gagcaatcag cgccacgttc gccatcagcg
    34021 cggtgatctt gatacaaacc gcctcttcga aggattcctg aattgcggcg tatcccacag
    34081 cgccaatgaa acagaggctt atgccctgca agaagcgcaa cagggtgaat tgttcaatgt
    34141 tttgcgccag taatattgcc agacaggtga cgataaacca caccacaccc gccagcatca
    34201 ccggacggcg accaatacga tccgacagcg gccccaggag ccattgtaaa aacatcccgc
    34261 ccgccagata cgcggtcatc gaagtaggaa cccaatcaat gcccgcctga tattgttcca
    34321 ccacggccaa cataccgggt tggatcatat cgttgccgat ataggttgaa aattcgtaaa
    34381 gcaccagaca gagagggaaa agtaacgcct gacgtccaag cctggcaccg aaagctaatt
    34441 tattttgcat gcaatttctt cgccaataat aatcgcgcag agtttaataa aagcgcagct
    34501 aacgagaaag cgaattttgt agctgaaacc acggttaagc acattcttac attattgcga
    34561 gtatagctac gctttcttta agttttattt aacctatgcc cgttacaatc acccaccgta
    34621 aacaggccgc ttgagggaaa taagacgatg ccgctttacc cagtttaacc tgcactttat
    34681 tctcaacgac ttgcctgtat tggctccctt ttaatcactt tgcgtcggga agttatgctg
    34741 gaaaatttga atctctctct attctctctt attaacgcga cgccagactc ggctccgtgg
    34801 atgatctcgt tggcgatttt tattgctaaa gatttgatta ccgtggtgcc gttgctggcc
    34861 gcggtacttt ggttgtgggg gcttacagca caacggcaac tggtgataaa aatcgctatc
    34921 gcgctggcgg tcagcctgtt tgtgtcctgg acgatgggac atctttttcc gcacgaccga
    34981 ccctttgtcg aaaatatcgg ctataacttc ctgcatcatg cggcggatga ctcattcccg
    35041 agcgatcacg gtacggtgat tttcaccttt gcactggcat ttttatgctg gcatcgcctg
    35101 tggtccggct cacttttaat ggttctggcc gtcgtcattg cctggtcgcg cgtttatctt
    35161 ggcgtccact ggccgctgga tatgctcggt ggattgctgg caggtatgat tggctgcctt
    35221 agtgcccaga ttatctggca agcgatgggg cataaactct atcaacgtct gcaatcgtgg
    35281 tatcgcgtct gttttgcatt accgatccgc aaaggctggg tgcgtgactg atctctgcta
    35341 aaaagtgtag tattgagcgg ctcgcttcaa taactattca gagggattat ggaaacacgt
    35401 cgcgaagagc gtatcgggca gctgctgcaa gaattaaaac gcagcgataa gttacatctt
    35461 aaagacgccg ccgccctgct tggggtttcg gagatgacga ttcgtcgcga tctgaacaac
    35521 cacagtgcgc ccgtcgtttt gctcggcggc tatattgttc tggaaccgcg cagtgccagc
    35581 cattacctgt taagcgatca aaaatcccgc ctggtggaag aaaaacgccg ggcggcaaaa
    35641 ctggctgcga cactggtaga acccgatcag accctctttt ttgactgtgg caccaccacg
    35701 ccgtggatta ttgaagcgat tgataatgaa atccctttta ccgccgtttg ttattcgcta
    35761 aatacctttc tggcgctgaa agagaaaccc cattgccgcg catttctgtg cggtggtgaa
    35821 tttcacgcca gcaacgccat tttcaacccc atcgattttc agcaaacgtt gaataatttt
    35881 tgcccggata tcgcttttta ttccgcggcg ggcgtgcatg tcagtaaagg cgctacctgt
    35941 tttaatcttg aagagttgcc ggtaaaacac tgggccatgt cgatggcgca aaagcatgtg
    36001 ctggttgtcg accacagtaa atttggcaag gtgcgtccgg cgcgcatggg tgacctgaaa
    36061 cgctttgata ttgtggtgag tgattgttgc ccggaagatg agtatgtgaa gtacgcgcag
    36121 acgcaccgta ttaagttgat gtattaatga cgtataaccg gatgacgttt cgcgccatcc
    36181 ggttatcaga agattaagag aaccagctgc cgaaccactg atggaatttc atcatcacga
    36241 aatcccacat ccgaccaaag aatccgccct cttccacatt ttccatcacg atcagcggac
    36301 gctgctcaat ggatttaccg ttaagctgga aatcaatggt cccgacaacc tgaccttttt
    36361 tcagcggtgc ggtaagctgc ggttccgtta acgtgtagct cgccttcagg tttttcagct
    36421 gcccacgcgg tatggtcacg gagcccgctt cgcctgcccc gagattcact tcgctcttat
    36481 caccaaacca gacgcgctga gtcacaaagg tggcatcagg tttaattggc gtcacagttt
    36541 caaagaagcg gaaaccccag gtcaataatt tctcagactc attaaaacgg atacggtcag
    36601 ttttcgcccc cagcactacg gagattaaac gcatatcacc ctgggtagcc gaagcaacca
    36661 gattatatcc cgcacctgcc gtggttcctg tcttcatgcc atcaacattc agattgctgc
    36721 tccacagcag acggttacgg ttaggctgac gaattttgtt gaaggtgaac tctttctctt
    36781 tatgaatggc gtactcttcc ggcacatcgt ggatcaatgc tttacccagc aatgccatat
    36841 cgcgcgcggt actgaactgc cccggcgcat ccagaccgtg caccgtctgg aaggtagtgt
    36901 tggtcagacc cagttttttg gcataaccat tcatcaaacc aataaatgac tcctggctcc
    36961 cggcaacgta atcggccagc gcaatacagg cgtcattacc ggactggata atcacacctt
    37021 tgttcaagtc tgccaccgaa acctgatcgc ccggtttgag gaacattacc gatgaaccac
    37081 gcagtgccgg atttcccgtc gcccaggcat ctttaccgac cgtcaccata tcggtgagtt
    37141 taatcttatc ggccttaagc gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc
    37201 tcgcgggatc cagtttctca tccgcgttgc cttctgccag cactttaccg ctggcgtaat
    37261 ccattaaaat ccatgcacgc gcatccacgc tcggcgcttc aacggtttgt tccgccgcga
    37321 atgccgttgg ggcaaaaagg aataaaaatg cagaacccgc tgcaagacca cgaaggagag
    37381 aggagtattg cgtcataaat gccacccgag tatccattca aaaaaaatac gttatatcgc
    37441 cgtatgataa gttcggtgat taataacgca cacacaaact taaagaaacc gtaagttggt
    37501 aaagttttta aagtttatgc aatatcctgc cgcttcgctg cttctggtgt catttttttg
    37561 ccagctgttg cttaaaagtt aactttactt caccatcgga gtcctgttca gcctgtggct
    37621 gcctgtcgga aaaggggtga gtatgattac gctgtggggt cggaataatt caactaacgt
    37681 aaaaaagtat tgctgacgct cgaagaactg gaactacctt atgagcaaat tctcgcgggc
    37741 cgtgagtttg ggataaatca cgatgctgat tttctggcga tgaaccctaa cgggctggtg
    37801 ccgttgttgc gtgacgacga aagtgatctc attctttggg aatcaaacgc cattgtccgc
    37861 tatctggcgg cacagtacgg gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg
    37921 gaagcggaaa aatggatgga ctgggcaaac cagacgctca gtaatgctca tcgcgggatc
    37981 ctgatgggat tagtcagaac accaccggaa gagcgcgatc aggccgccat tgatgccagt
    38041 tgcaaagagt gcgacgccct gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg
    38101 ttctctggcg acgagtttgg tgtgggcgat atcgctatcg caccgttcat ctacaatttg
    38161 tttaacgttg gcctgacctg gacaccgcgt ccaaatctgc aacgctggta tcagcaactc
    38221 actgaacgcc ccgcagtgcg caaagtggtg atgattcccg ttagctaatt gcgtgggcta
    38281 actttaagta attccccact ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca
    38341 gtgcgaacat cacgaattcg ctgccctctg tccgttaaaa tacggccatc ttctgtcact
    38401 ttgtcgccgt tgacgctcat cacaatgaca tctttatctt tcagcgcgcc aataaataat
    38461 ttttgctgcc actgggggaa tttatcgctg ttatagaagg ccatgccgct cacagcgggc
    38521 gaatctttcc agtaaaaaac aggttgctcg gtcccggcga cgatctcccc tttcgcttcc
    38581 ggtatcttaa agcctgaata gttgattccc caggttgcca gcggccagcc gtagtttttg
    38641 cctttttgcg ggatattaat ttcatcacca ccgcgcgggc catgttcatt cagccacagt
    38701 gcattactcc acggattcat cgccattcct tgcggattac gaatgccata agaccagatc
    38761 tcggcgcgcg caccggattc ctttataaaa ggattatcat ccgggatttc gccctggtcg
    38821 gtcagacgca ccagtttgcc ctgtaattta tccagatcct gcgccgtcgg gcgctgattg
    38881 ttttcgccca gagcaataaa aagataacct ttaccgtcga ataccagccg cccgccaaaa
    38941 tggttgccgg tagacagttt tggcatctgg cggaagacgg tgcggaagtc ggtcactttt
    39001 gagagatcat cacttaagcg gccataaccc acagcagttc cggctttgcc atcatcgcca
    39061 acttcggaat aacttaacca gatgcggcga gactgagcaa aatcaggcgc taaaaccacg
    39121 tccagcaggc cgccctgccc gtgcgcccaa acgtccggaa ctccggaaag cggcgcagat
    39181 aatccttttc ctgcttgcca gtgacgcaac tcgccgccgc gcagagtgat taacataccg
    39241 tgattatcgg gtaaaaaggc cagtgcccag ggatggtcga gtttgtcttg cagtacttcg
    39301 acatttaccg ttgcaggagc cgcccagaga gcggaagaaa gacaaataag gggcacaagg
    39361 aaaaaggatt gtcgatgcat aacgcctccg gattgactct ggcttaaagc gtagtcagtg
    39421 gaggagataa caaattcatt tttacaaaaa cttaaacatg aagggggaga cgctttctcc
    39481 cccttagttt tcaggccttc tcaagcatgg cgtgcttctg caggctctgg atactcagcg
    39541 ttaagctcat cagacaattt tcaagcttat cggcgttgac ggtaataaca gtcgggcaat
    39601 catggtgccc actcatcaaa catactgcgg ctgtcgctaa tgcttcttca gcatgatgaa
    39661 gagcactcca ctcttcctga tccagatgaa gattcaaccg cagcgattta tcgtgcagtt
    39721 cgcgattcag tttaaaaaag ttatctcgta gatgattgct ttcgctgacg gacatgtatc
    39781 cttttgcctt cctcagttgc acgtatgcag cgaggtcaac gcgtgcgtcg atcaaccggt
    39841 tcagcagatc gattcgctgt ctgtcaacga acatacttcg atcctcctct ttccagcctt
    39901 gttgcgatta aatagtatgg ctattttttg agcaaaatgc tgtccaggat caattcttca
    39961 cgccaaatta acaaaattta catttccttc gcctccgcca gcgaatgaga ttgcgataca
    40021 aattttatcc agaactctgt agaatccctg ccctgacctt tcctgtaact gaatattttt
    40081 taagctatga gcaaagtaac tccccagccg aaaatcggct ttgtttccct tggctgtccg
    40141 aaaaaccttg tcgattcaga gcgtattctc accgaactac gcactgaagg ttatgacgtg
    40201 gtaccgagct atgacgatgc ggacatggtg atcgtcaaca cctgcggctt tattgacagc
    40261 gcggtacaag aatcactgga agccattggt gaagcgttga atgaaaacgg caaggtaatt
    40321 gtgaccggtt gtctgggggc aaaagaagat cagatccgcg aagtccaccc gaaagtgctg
    40381 gaaatcaccg ggcctcatag ctatgagcag gttctggagc acgttcatca ctacgtgcca
    40441 aaaccgaaac acaacccatt cctgagcctg gtgccagaac aaggtgtgaa actgacaccg
    40501 cgtcattatg cctatctgaa aatttcggaa ggctgtaatc accgctgcac cttctgcatt
    40561 attccgtcta tgcgcggcga cctggtgagc cgtccgattg gcgaagtgtt aagtgaagcg
    40621 aaacgtctgg tagatgcggg cgttaaagag attctggtga tctcgcagga tacttccgcc
    40681 tatggcgttg atgttaaaca tcgtactggc ttccacaacg gcgagccggt aaaaaccagc
    40741 atggtcagcc tgtgcgaaca gttatcgaaa ctggggatct ggacacgtct gcactacgtt
    40801 tacccttatc cgcatgtgga cgacgtcatc ccactgatgg cagaaggcaa aatcctgccg
    40861 tatctggaca ttccgttgca gcacgccagc ccgcgcattc tcaaactgat gaagcgtccg
    40921 ggttctgtag atcgccaact ggcgcgcatc aaacagtggc gcgaaatctg cccggaactg
    40981 accctacgct caacctttat tgtcggcttc cctagcgaga cggaagaaga tttccagatg
    41041 ctactcgact tcctgaaaga agcgcgtctg gatcgcgttg gctgctttaa atacagcccg
    41101 gttgaaggtg cagacgccaa tgccctgcct gaccaggttc cggaagaagt gaaagaagaa
    41161 cgctggaacc gtttcatgca gctgcagcag cagatttccg ccgagcgcct gcaagagaaa
    41221 gtgggccgtg aaattctggt gattatcgac gaagtggacg aagaaggcgc gattggtcgc
    41281 agcatggcag atgcaccgga aatcgacggc gcggtttatc tcaatggtga aaccaacgtt
    41341 aagccgggtg atatcctgcg tgtgaaagtc gagcacgccg atgagtacga tttgtggggt
    41401 agccgggttt aattaattca ggccgggcaa aatcatttgt ccgccctgcg aataatgatt
    41461 actgccagct aatctgagcc ctgccgtttt tcttactctt atacagccgc tcatccgcca
    41521 gcattttcaa gcgttcgacg gtggtacatt catttgaatg cgctacaccg atactggtac
    41581 ttaaattaat gcttcccacc tggaaatgaa cgatttcttt tagaatcgat agcaactctt
    41641 ccagcgttcc acccgtataa atcaccgcaa attcgtcacc gccgaagcgg tagattttac
    41701 ctttatctac cgggatctgt gatttaagga ttttcaccac tttcatcaac acttcatcgc
    41761 ccagcaaatg accgaaggtg tcgttaatgt ttttgaattt atcgacgtca aacaggcaca
    41821 aataaccgcc tttttccggc agcgctttta aatcttcgtc aaaggatttt ctgcccattg
    41881 ctttggtcag gtcgtccagg aacgccgagt tatacatact gtagagtttt ttgacatcgg
    41941 cagccgaaaa gaaaagcccg ccatccagta cgcgttcaaa atggttatta atgatggctt
    42001 ctttctccac aataccacga ttaatcactc tgcgtaccag atagaggcaa ctggtacccg
    42061 atatcactgc caccaatgcg gcaaagaaaa taatcaatgt acagacccgc aaataaacat
    42121 cttttttata cgcaaatacg tacaatccgg tcggcaaata ggcgaagtgc tccatcgctt
    42181 ccgaaacgat gaagatggcg tttttagcat tatattcatc accggtttac cgttcttaaa
    42241 tagagagaac atcaggttct cgctgtccat cgccagcgat tttgacagta aagaaaaacg
    42301 ctccagacca agcaaaaata tcagactacg attgtgagtt accggataga ggtaatagac
    42361 gaaggtttta ccgtcaaaag agataaaccc tgtatttttg acatgtcgat acttcacaac
    42421 ctgcataata tctgcacaat aatttttctc gaactccttt acctgaatac attcgtcact
    42481 ggaaatcaga taaggaccga catcgtacaa ataagagggg gaaaccgagt cggcgtaagc
    42541 tgtgcgcaaa ccaaccaaca gctgttcctc cgtgggattt agcgtgttga taagatcaat
    42601 tgccacgcta cgccgatatt cagcaatatt tgccaccgca aaagcagtat caataacgga
    42661 ctgtttcagg ctatacaccc gttcctttac catttgagtg tagatcccgc aggcaactgc
    42721 tgaaatcatg ataaaaatca gcagactgac tgtaagtacg aacttattga ttctggacat
    42781 acgtaaacta ctcttttact aattttccac ttttatccca ggcggagaat ggcatgggtt
    42841 tctggtacag atacccctga gcctgaacac atcctaattt aattaattcg cgcgcaacgt
    42901 cttcgctctc tacgccttcc gcaatcaccg ggatccggtt atagcgtgcc agatcgaaaa
    42961 taatgcgcac aatttttccc gcagtaccga aaatatcgcc ggtaaattgc gagtccacct
    43021 ttatgctgtc tggattaatt tcataaaaac gtttcaggtt tgacaaaccg gtgccaaagt
    43081 catcaatcga tatccgtacc ccttgtttac gcagttgttc aatattgaga taaaaatgtt
    43141 cattaattcg ctcgatcacg gtttcagtga tctcaacaca gaggctgttc gccttcaggc
    43201 cattttcttt catgatggtg gtaaaacgct catgaaagtg cggttcgttt aactgccagg
    43261 gcgaaacatt aatatgcagt aagaaatcat ccttcagcga cgcacgctga ctaaaatgtc
    43321 tgaattcacc cattgccgtt ttaataatct gataacccag ctcattgata aacccgatat
    43381 cttcagcaat ggtaatgaac ttcagcggtg aaatgatccc ccgctccgca gaaacccaac
    43441 ggcacaacgc ctccgcgccc agaatggcgc gggtgttaat atcaacgatc ggttgataga
    43501 cgaggaatag ttcgccaatt tccattgcat ggcgaatatt acgcgcaatc cagatattct
    43561 cttctatttc gttgagtttc gcgtcatcac agaaaataaa ctcggagaac atatggtcac
    43621 ggatactgga aatcgccagt ttcagttttt ctgcgtaact ccaggcgttt tccccctgca
    43681 aaggttcaca aatggcagac ttatggatgc gcagattgcg gtagccaaag ctgctgtcaa
    43741 aatcgtttac catcgctatt ttacgggtaa atgtctgtac attttcccga cacagaagta
    43801 aatagagatc ggcattgaat gtcacgatat cgcagcattg cgcatatttc tcggcaattc
    43861 gctgacacaa tttcctgacg attttattgc gttcatcaac accaaacacc gcttccacgg
    43921 cttccagacc acacagtttg atgatcccca cagccagata ctgattattg ttggactcaa
    43981 taatcgccat atttttaata aaaccgcttt cctgatcgta agagatcagt gagagcaatt
    44041 tatttgaccg ttcaatttcg gcatctttca tttccgcgat cgaa
//
LOCUS       DAESHI010000023        43399 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.023, whole genome shotgun sequence.
ACCESSION   DAESHI010000023 DAESHI010000000
VERSION     DAESHI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.023"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(2..94)
                     /locus_tag="KW011_001309"
     tRNA            complement(2..94)
                     /locus_tag="KW011_001309"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="KW011_001310"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="KW011_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBH7659694.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="KW011_001311"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="KW011_001311"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBH7659695.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="KW011_001312"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="KW011_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBH7659696.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4316..5377)
                     /gene="recA"
                     /locus_tag="KW011_001313"
     CDS             complement(4316..5377)
                     /gene="recA"
                     /locus_tag="KW011_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBH7659697.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5457..5954)
                     /gene="pncC"
                     /locus_tag="KW011_001314"
     CDS             complement(5457..5954)
                     /gene="pncC"
                     /locus_tag="KW011_001314"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HBH7659698.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6099..7184)
                     /gene="mltB"
                     /locus_tag="KW011_001315"
     CDS             complement(6099..7184)
                     /gene="mltB"
                     /locus_tag="KW011_001315"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HBH7659699.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7440..8003
                     /gene="srlA"
                     /locus_tag="KW011_001316"
                     /gene_synonym="gutA"
     CDS             7440..8003
                     /gene="srlA"
                     /locus_tag="KW011_001316"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBH7659700.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8000..8959
                     /gene="srlE"
                     /locus_tag="KW011_001317"
                     /gene_synonym="gutE"
     CDS             8000..8959
                     /gene="srlE"
                     /locus_tag="KW011_001317"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBH7659701.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            8970..9341
                     /gene="srlB"
                     /locus_tag="KW011_001318"
                     /gene_synonym="gutB"
     CDS             8970..9341
                     /gene="srlB"
                     /locus_tag="KW011_001318"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBH7659702.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9345..10113
                     /gene="srlD"
                     /locus_tag="KW011_001319"
                     /pseudo
     CDS             9345..10113
                     /gene="srlD"
                     /locus_tag="KW011_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            10219..10578
                     /gene="gutM"
                     /locus_tag="KW011_001320"
     CDS             10219..10578
                     /gene="gutM"
                     /locus_tag="KW011_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBH7659703.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10645..11418
                     /gene="srlR"
                     /locus_tag="KW011_001321"
                     /gene_synonym="gutR"
     CDS             10645..11418
                     /gene="srlR"
                     /locus_tag="KW011_001321"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HBH7659704.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11411..12376
                     /gene="gutQ"
                     /locus_tag="KW011_001322"
     CDS             11411..12376
                     /gene="gutQ"
                     /locus_tag="KW011_001322"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HBH7659705.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12373..13887)
                     /gene="norR"
                     /locus_tag="KW011_001323"
     CDS             complement(12373..13887)
                     /gene="norR"
                     /locus_tag="KW011_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HBH7659706.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            14074..15513
                     /gene="norV"
                     /locus_tag="KW011_001324"
     CDS             14074..15513
                     /gene="norV"
                     /locus_tag="KW011_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HBH7659707.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTKSDLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15510..16643
                     /gene="norW"
                     /locus_tag="KW011_001325"
     CDS             15510..16643
                     /gene="norW"
                     /locus_tag="KW011_001325"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HBH7659708.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(16771..19023)
                     /gene="hypF"
                     /locus_tag="KW011_001326"
     CDS             complement(16771..19023)
                     /gene="hypF"
                     /locus_tag="KW011_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HBH7659709.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19176..19703)
                     /gene="hydN"
                     /locus_tag="KW011_001327"
     CDS             complement(19176..19703)
                     /gene="hydN"
                     /locus_tag="KW011_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HBH7659710.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19851..20861)
                     /gene="ascG"
                     /locus_tag="KW011_001328"
     CDS             complement(19851..20861)
                     /gene="ascG"
                     /locus_tag="KW011_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HBH7659711.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            21121..22578
                     /gene="ascF"
                     /locus_tag="KW011_001329"
     CDS             21121..22578
                     /gene="ascF"
                     /locus_tag="KW011_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HBH7659712.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            22587..24011
                     /gene="ascB"
                     /locus_tag="KW011_001330"
     CDS             22587..24011
                     /gene="ascB"
                     /locus_tag="KW011_001330"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HBH7659713.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24125..24544)
                     /locus_tag="KW011_001331"
     CDS             complement(24125..24544)
                     /locus_tag="KW011_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH7659714.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(24541..24852)
                     /locus_tag="KW011_001332"
     CDS             complement(24541..24852)
                     /locus_tag="KW011_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HBH7659715.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(25014..25787)
                     /locus_tag="KW011_001333"
     CDS             complement(25014..25787)
                     /locus_tag="KW011_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659716.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            complement(25812..26282)
                     /gene="hycI"
                     /locus_tag="KW011_001334"
     CDS             complement(25812..26282)
                     /gene="hycI"
                     /locus_tag="KW011_001334"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HBH7659717.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(26275..26685)
                     /gene="hycH"
                     /locus_tag="KW011_001335"
     CDS             complement(26275..26685)
                     /gene="hycH"
                     /locus_tag="KW011_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HBH7659718.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(26682..27449)
                     /gene="hycG"
                     /locus_tag="KW011_001336"
     CDS             complement(26682..27449)
                     /gene="hycG"
                     /locus_tag="KW011_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HBH7659719.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(27449..27991)
                     /gene="hycF"
                     /locus_tag="KW011_001337"
     CDS             complement(27449..27991)
                     /gene="hycF"
                     /locus_tag="KW011_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HBH7659720.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(28001..29698)
                     /gene="hycE"
                     /locus_tag="KW011_001338"
     CDS             complement(28001..29698)
                     /gene="hycE"
                     /locus_tag="KW011_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HBH7659721.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            complement(29716..30639)
                     /gene="hycD"
                     /locus_tag="KW011_001339"
     CDS             complement(29716..30639)
                     /gene="hycD"
                     /locus_tag="KW011_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HBH7659722.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            complement(30642..32468)
                     /gene="hycC"
                     /locus_tag="KW011_001340"
     CDS             complement(30642..32468)
                     /gene="hycC"
                     /locus_tag="KW011_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HBH7659723.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(32465..33076)
                     /gene="hycB"
                     /locus_tag="KW011_001341"
     CDS             complement(32465..33076)
                     /gene="hycB"
                     /locus_tag="KW011_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HBH7659724.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(33201..33662)
                     /gene="hycA"
                     /locus_tag="KW011_001342"
     CDS             complement(33201..33662)
                     /gene="hycA"
                     /locus_tag="KW011_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HBH7659725.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            33874..34224
                     /gene="hypA"
                     /locus_tag="KW011_001343"
     CDS             33874..34224
                     /gene="hypA"
                     /locus_tag="KW011_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBH7659726.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            34228..35100
                     /gene="hypB"
                     /locus_tag="KW011_001344"
     CDS             34228..35100
                     /gene="hypB"
                     /locus_tag="KW011_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HBH7659727.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            35091..35363
                     /gene="hypC"
                     /locus_tag="KW011_001345"
     CDS             35091..35363
                     /gene="hypC"
                     /locus_tag="KW011_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HBH7659728.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            35363..36484
                     /gene="hypD"
                     /locus_tag="KW011_001346"
     CDS             35363..36484
                     /gene="hypD"
                     /locus_tag="KW011_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HBH7659729.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            36481..37491
                     /gene="hypE"
                     /locus_tag="KW011_001347"
     CDS             36481..37491
                     /gene="hypE"
                     /locus_tag="KW011_001347"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HBH7659730.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            37565..39642
                     /gene="flhA"
                     /locus_tag="KW011_001348"
                     /pseudo
     CDS             37565..39642
                     /gene="flhA"
                     /locus_tag="KW011_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(39679..40032)
                     /locus_tag="KW011_001349"
     CDS             complement(39679..40032)
                     /locus_tag="KW011_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HBH7659731.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(40109..40243)
                     /locus_tag="KW011_001350"
     CDS             complement(40109..40243)
                     /locus_tag="KW011_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659732.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            40319..42880
                     /gene="mutS"
                     /locus_tag="KW011_001351"
     CDS             40319..42880
                     /gene="mutS"
                     /locus_tag="KW011_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBH7659733.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            42986..43399
                     /locus_tag="KW011_001352"
                     /pseudo
     CDS             42986..43399
                     /locus_tag="KW011_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
BASE COUNT        10273 a        11917 c        11305 g         9904 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatcg tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 aacgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagatccat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaacatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatccct acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gagtgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcacca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata acctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgacccaggt
     4141 tttgctgata aacagattta atagtcaatg agtgtgcagc caattcgtta gaggatcgag
     4201 gtgagagtaa tttttcacgc cagtgttggc ctgttcccct caccctaacc ctctccccaa
     4261 aggggcgagg ggaccgtccg tgcacacatt atctattatc atcaataacg aacgattaaa
     4321 aatcttcgtt agtttctgct acgccttcgc tgtcatctac agagaaatcc ggcgttgagt
     4381 tcgggttgct cagcagcaac tcacgtactt tcttctcgat ctctttcgcg gtttccgggt
     4441 tatctttcag ccaggcagtt gcattcgctt taccctgacc gatcttctca cctttgtagc
     4501 tgtaccacgc gcctgctttc tcgatcagct tctcttttac gcccaggtca accagttcgc
     4561 cgtagaagtt gataccttcg ccgtagagga tctgaaattc agcctgttta aacggtgcag
     4621 cgattttgtt cttcaccact ttcacgcggg tttcgctacc caccacgttt tcgccctctt
     4681 tcaccgcgcc gatacgacgg atgtcgagac gaacagaggc gtagaatttc agcgcgttac
     4741 caccggtagt ggtttccggg ttaccgaaca tcacaccaat tttcatacgg atctggttga
     4801 tgaagatcag cagcgtgttg gactgcttca ggttacccgc cagcttacgc atcgcctggc
     4861 tcatcatacg tgccgcaagg cccatgtgag agtcgccgat ttcgccttcg atttccgctt
     4921 tcggcgtcag tgccgccacg gagtcaacga cgataacgtc tactgcgcca gaacgcgcca
     4981 gggcgtcaca gatttccagt gcctgctcgc cggtgtccgg ctgggagcac agcaggttat
     5041 cgatatcgac gcccagttta cgtgcgtaga ttgggtccag cgcgtgttca gcatcgataa
     5101 acgcacaggt tttaccttca cgctgcgctg cggcgatcac ctgcagcgtc agcgtggttt
     5161 taccggaaga ttccggtccg tagatttcga cgatacggcc catcggcaga ccacctgccc
     5221 caagcgcgat atccagtgaa agcgaaccgg tagagatggt ttccacatcc atggaacggt
     5281 cttcacccag gcgcatgatg gagcctttac caaattgttt ctcaatctgg cccagtgctg
     5341 ccgccaacgc tttctgtttg ttttcgtcga tagccatttt tactcctgtc atgccgggta
     5401 ataccggata gtcaatatgt tctgttgaag caattatact gtatactcat acagtatcaa
     5461 gtgttttgta gaaattgttg ccacaaggtc tgcaatgcat acgcagtagc ctgacgacgc
     5521 accgcatcac ggtcgccgct gaagcattcc cgccgggtaa tgccttcacc gcgggcagtg
     5581 gcaaaagcaa accagacggt gccgacaggc ttctcttcac tgccgccatc cggcccggcg
     5641 ataccactaa tagacacggc ataatcagca cgagccgctt tcagtgcacc tatcgccatt
     5701 tccaccacga cgggttcact caccgcgcca tgctgcgcca gcgtctcttc gcgtacgccg
     5761 atcatctgcg ctttggcttc gttactgtag gtgacaaatc cgcgttcaaa ccaggcggag
     5821 ctaccggcaa tatcggtaat cactttcgct acccaaccac cggtacaaga ctcggcagtt
     5881 gttacggttg cgccacgggc tttcagcgcc tgcccaacct gttcgcttaa ctgcatcagt
     5941 tcactgtcag tcatcacaac ctcagtcagt caaaaattta tgccggacaa gatagcactt
     6001 tcgtcaggaa gatggtgttg gcgttgcaat tttacgagga ggattcagaa aaaagctgat
     6061 tagccagagg gaagctcacg cccccctctt gcaaatagtt actgcacgcg cgccagcgcc
     6121 acggcttgtc ctaactgcca gaccgccatt gcgtaatggg tgctgtggtt ataacgggta
     6181 atggtgtaga agttcggcag accgtaccag tactggtaac cggtaccgac atccagacgc
     6241 agcaggctgg cttgttgatg gttgcccagc ggctgctgtg gcgttaaacc tgccgtggca
     6301 agctgcgaaa tgctgtacct ggttttgaag ccatttggca agcctggagc ctgaccgttt
     6361 gccattaccg cgacctgatc gcctttcacc cagccgtgcg ctttgaaata gttcgccaca
     6421 ctaccgatcg catcaaccgg atcccacagg ttgatatgcc cgtcgccgct gaaatctacc
     6481 gcatattgtt tgtaagacga cggcataaac tgtccgtagc ccatcgcccc ggcaaaggaa
     6541 cctttcagat tgagcggatc gtcctgctcg tcgcgcgcca tcaacaggaa ggtttccagc
     6601 tcgccagaga aatactccgc gcggcgtggg tagttaaatg acagcgttgc cagcgcatcg
     6661 aggatgcgag ttttccccat cacgcgcccc cagcgggttt caacgccgat aatcccgacg
     6721 ataatttccg gcggtacgcc atacacctgc cacgcgcgat tcaacgcatc ttcatactga
     6781 ttccagaaaa ccacaccgtt ctgcacgttg tccggcgtaa taaatttttt gcgataacgc
     6841 agccatgcgc cgttcggacc tgatggcggt ttcaccgatg tggttggtgc ctggttatcc
     6901 atcagccgca gtaccgaatc cagacgcttc gcctgggaga gaatttcctg caactgctga
     6961 cgatcgaaac cgtgtttgtt caccattctg tcgatgaact gctgggcatt cgggttatta
     7021 gcgaaatcgc cgcccatctg catcacattg tgctgaggct caagcaggaa gccgccagac
     7081 ggcgttccgg tggtcgtttc agtctcagta ggttttggct tgctgctaca ggcggcaagc
     7141 aacacaaaaa ggggaagcaa tgttacataa cgacgcttga acatgagggg tccatttaac
     7201 agattcaacc aggggcaagt atggtaaagc atcacgcccc gcacaaggaa gcggtagtca
     7261 ctgcccgata cggactttac ataactcaac tcattcccct cgctatcctt ttattcaaac
     7321 tttcaaatta aaatatttat ctttcatttt gcgatcaaaa taacactttt aaatctttca
     7381 atctgattag attaggttgc cgtttggtaa taaaacaata aatcctgaag gagagaacaa
     7441 tgatagaaac cattactcat ggtgcagagt ggtttatcgg gctgttccaa aagggcggag
     7501 aggtgtttac cgggatggtg accggcattc ttccgctgtt gattagcctg ctggttatca
     7561 tgaacgcact gattaatttt atcggtcagc atcgtattga acgttttgct caacgttgcg
     7621 ccggtaaccc tgtttcccgt tacctgctgc taccgtgcat tggcacgttt gtcttttgca
     7681 atccgatgac cttaagcctg ggtcgcttta tgccggaaaa gtacaaaccc agctactacg
     7741 cggcagcctc ttatagctgc cactcaatga atggcctctt tccccatatc aaccctggcg
     7801 aactgtttgt ttatcttggc attgccagcg gtctgacaac gctgaacctg ccacttggcc
     7861 cactggcggt gagctatctg ctggttggtc tggtcaccaa cttctttcgc ggctgggtga
     7921 ccgatctgac caccgccatt tttgagaaaa agatgggcat tcaacttgaa caaaaagttc
     7981 acctggcagg agcaacatca tgacgcgtat tcggatcgaa aaaggaacgg gtggctgggg
     8041 cggcccgctt gagctggaag ccacgccggg caaaaaaatc gtctatatca ccgccggtac
     8101 ccggcctgcg attgttgaca aactggcaca acttactggc tggcaggcta ttgacggatt
     8161 taaagaaggt gaacccgcgg aggcggaaat tggtgtcgcg gtaatcgact gtggcggcac
     8221 attacgctgc ggcatctatc cgaaacgacg tattcccacc attaatatcc actcgacggg
     8281 caagtccagt ccgctggcgc agtacattgt ggaagatatt tatgtctctg gcgtaaaaga
     8341 agaaaacatc actgtagtgg gtgatgcgac accacaaccc tcttccgtgg gccgtgacta
     8401 tgacaccagc aagaaaatca ccgaacaaag cgatggttta ctggcgaagg tgggaatggg
     8461 tatgggttct gccgttgccg tgttgtttca atctggtcgt gacaccatcg acactgtatt
     8521 aaaaaccatt ctgccgttta tggcgttcgt ctcggcgctc attggcatca ttatggcttc
     8581 tggccttggt gactggattg cccacggtct tgctccgctg gcgagccatc cactgggtct
     8641 ggtcatgctg gcgctcatct gctccttccc gctgctttca cctttcctcg gcccaggcgc
     8701 agttatcgca caggttatcg gcgtattgat tggcgtgcag attggtctcg gcaatattcc
     8761 gccgcatctg gctttaccgg cactgtttgc catcaacgcg caggcggcct gcgacttcat
     8821 cccggtcggt ttgtcgctgg cggaagcccg tcaggacacg gttcgcgtcg gtgtcccttc
     8881 tgtactggtg agccactttt taaccggcgc accgactgta ctgatcgcct ggtttgtctc
     8941 cggttttatc tatcaataga ggctgaaaca tgaccgttat ttatcagacc accatcaccc
     9001 gtatcggcgc gagtgccact gacgccctca gcgaccagat gctcatcacc tttcgtgaag
     9061 gcgcgccagc ggacctcgaa gagtattgct tcattcattg ccacggcgag ttgaaaggtg
     9121 cactccatcc cggtttgcaa tttacgctcg ggcagcatcg ctatccggtg accgctgttg
     9181 gcagcgtggc ggaagacaac cttcgcgaac tgggtcatgt caccctgcgc ttcgatggtt
     9241 taaacgaagc ggaatttccg ggcactgtcc atgtggcagg ccctgtaccc gacgatatcg
     9301 cgccgggatc ggttttgaag tttgaatctg ttaaggagta aaaaatgaat caggttgccg
     9361 ttgtcatcgg tggtgggcaa accttaggcg cgttcctgtg ccacggtctg gctgccgagg
     9421 ggtatcgcgt cgcggttgtc gatattcaga gcgacaaagc cgcaaatgtg gcacaagaaa
     9481 ttaacgccga atatggtgaa ggaacagcgt atagttttgg tgctgacgcc accagcgagc
     9541 aaagcgtcct ggcgctctct cgtggggtag atgaaatctt tggtcgcgtg gatttgctgg
     9601 tctacagcgc cggaatagca aaagcagcct ttatcagcga cttccagctc ggcgattttg
     9661 accgtgaatc tggtgggtta tttcctgtgt gcccgtgaat tttctcgttt gatgatccgc
     9721 gacgggattc agggtcgcat tattcagatc aactcgaaat ccggcaaagt gggcagcaaa
     9781 cacaactctg gctacagcgc agcgaaattt ggtggcgtcg ggctgactca atcactggcg
     9841 ctggatctgg cggagtacgg cattacggtg cattcactga tgctcggtaa cctgctgaaa
     9901 tcgcctatgt tccagtcact gttaccccaa tacgcgacca agctgggtat caaaccggat
     9961 caagtcgagc agtattacat cgacaaagta ccgctcaaac gcggctgcga ttatcaggat
    10021 gtgctgaata tgctgctgct ctatgccagt cctaaggcgt cgtactgcac cggacagtcg
    10081 atcaatgtca ccggcggtca ggtgatgttc taatcaacag cggagatccg ttaaggatct
    10141 ccgtgagact aatagaatgc ctgatgcgct acgctcatca ggcatacagg acttccgcca
    10201 ctacattaag gaaaagttat ggtatccgca ctcatcaccg tcgccgttat cgcctggtgt
    10261 gcgcaactgg ccttaggcgg ctggcaaatt tctcgtttta accgtgcctt cgacacacta
    10321 tgccagcaag ggcgggttgg cgtgggccgt tccagcgggc gctttaaacc acgggtcgtg
    10381 gtcgccatcg ccctggacga tcagcagcgc atcgtcgaca ccttgtttat gaaaggactg
    10441 accgtcttcg cacgaccgca aaaaattccc gcaattaccg gtatgcatgc gggtgattta
    10501 cagcccgatg tgatctttcc ccatgatcca ctatcacaga atgctctatc attggcgctt
    10561 aaactgaaac gtggataatt tcgttgtgaa tgttacttgc ttgcgaagtt atcattttga
    10621 aacctaaatc aggtaatcac gcccatgaaa cctcgtcagc gtcaggccgc cattctggag
    10681 tatctgcaaa agcagggtaa atgctcggtt gaagaattgg cgcaatactt tgacaccaca
    10741 ggcacaacca ttcgcaaaga tctggtcatt ctggaacatg caggaaccgt cattcgtact
    10801 tatggcggag tggtgttgaa taaagaggaa tccgatccgc ctatcgatca taaaacactc
    10861 atcaacaccc acaagaaaga gctgatagca gaagctgccg ttagttttat tcatgatggc
    10921 gattcgatca ttcttgatgc tggcagtacc gttttgcaga tggttcccct gctctcgcgc
    10981 tttaataaca tcacggtgat gaccaacagt ctgcatatcg tcaatgcgct atctgaactg
    11041 gataacgaac aaactatcct gatgccaggc ggaacgtttc gtaaaaaatc ggcctcattt
    11101 cacggacagc tggcagagaa tgccttcgaa catttcagtt tcgataaatt gtttatgggc
    11161 accgacggca tcgatctcaa tgcgggcgta accaccttta acgaggttta taccgtcagt
    11221 aaggcaatgt gcaatgccgc gcgcgaagtg attttgatgg cggactcatc aaagtttggc
    11281 cgtaaaagcc ccaacgtagt ttgcagtctt gaaagcgtcg ataagctgat taccgacgca
    11341 ggtatcgatc cggcgtttcg tcaggcgctg gaagagaaag ggatcgatgt gatcataacc
    11401 ggagagagca atgagtgaag cactactgaa cgcgggacgt cagacgttaa tgctggagtt
    11461 gcaggaagca agccgtttac cggaacgtct gggcgatgat tttgttcgcg ccgccaatat
    11521 catcctgcac tgcgaaggca aagtggtggt ttcgggaatt ggcaaatcgg gccacattgg
    11581 taagaaaatc gccgcaacac ttgccagtac cggcacatcg gctttttttg tccatccggc
    11641 agaagcactg cacggcgatc tggggatgat tgaaagccgc gatgtgatgc tgtttatctc
    11701 ttactccggt ggcgcgaagg aactggatct gattattccg cgtctggaag ataaatctat
    11761 cgcgctgctg gcgatgaccg gcaaaccgac gtcaccgctg ggcctggcgg caaaagcggt
    11821 gctggatatc tccgtagaac gcgaagcctg cccgatgcac cttgcgccga cctccagcac
    11881 cgtcaatacc ctgatgatgg gtgacgcgct ggcgatggcg gtcatgcagg cgcgcggatt
    11941 taatgaagaa gattttgccc gctcccaccc agccggggca ctgggcgctc gcttgctgaa
    12001 taaagtgcat catctgatgc gccgtgacga tgccatccca caggtggcgt taaccgccag
    12061 cgtgatggat gcgatgctgg aactcagccg caccggtctg gggctggtgg cggtatgtga
    12121 cgctcaacaa caggtacaag gcgtctttac cgacggcgat ttacgtcgct ggctggtagg
    12181 cggcggcgca ctcaccacgc cagttaatga agcgatgacg acaggcggca ccacgttaca
    12241 ggcgcaaagt cgcgccatcg acgccaaaga gatcctgatg aagcgcaaaa tcactgccgc
    12301 accggtggtg gatgaaaacg gcaaactcac cggcgcaatt aacctgcagg atttctatca
    12361 ggccgggatt atttaatcct tcattcccag acgtttcgcc agccgatgca ggttggcgac
    12421 gtcggtttcc agcatccgcg cgcaggcagc ccagttatga tgattttgtg ccagtgcctg
    12481 gcgaatagtt tcacgctgga acgcttctgt cgcttcacgc aggttttgct taacaacggg
    12541 caccgccgcc gcttctggcg gcggcaacgt cacctcagga aaagcaaaat gttgcgcctc
    12601 aagaatcact tcatcgccgt tgcgggtggc tctcgccagc actaccgccc gatgaatagc
    12661 atgttccagt tcgcgcacgt tccccggaaa acgatagtgt tgcagtaaat ttcgcgctcc
    12721 ggcacttaat accacgcggg agagccccag ccgcaaacga cactgctcgc agaaataccc
    12781 cgccagcaga atgacatcat cgccccgctc acgcagcggc ggcaccgaaa gtggaaacac
    12841 gctcaggcga tgaaacaagt cagcgcgaaa tcgccctgcc atcacctctt cgcgtaagtc
    12901 gcggttagtc gccgccagca cgcgcacatc gacccgcaaa ctacggtcat cgccaacgcg
    12961 ctgaatatcg ccatactgca acaccctcag cagcttggcc tgcaatgcca acgacaactc
    13021 gccgatctca tccagaaaca gagtgccgtt atccgccatt tcaaacttcc cgctgcggtt
    13081 actgatagcg ccagtaaacg ctcctttcac atgcccgaac aactcacttt ccgccacact
    13141 ttccggcagt gcagcacagt tgagatagac cagcggattc accgcccgtg gcgaggcttc
    13201 atgaatcgct ttcgccacca gctccttacc ggttcccgtc tcaccgctga tcaggacgtt
    13261 gagatcggac gccgccacaa tctcaatctc ttttttcagt tgcgtcatgc caggggacaa
    13321 gccaatcatc tgcgtctgtt tcaccgcttc aaacggcgtg gcatcgcctg gcatcatatt
    13381 ctggctttcc agttgttcaa tcagcaacgc attgcttaac gctcccgccg ccagcgcagc
    13441 aatcagccgt agctcttcgt cgctgaaaac atcgaactga tcgggctgca tcccgtcgag
    13501 cgtcagtgcg ccgatcaggt tttgcccggc aaacaatggc agaccaacgc aggcgtgaac
    13561 cttcagactc tcctgcccag gaatcaaacc gtcataggga tcgggcaatt cgctgtctgc
    13621 gggaaagcgc accacatccc cggcgcgggc aatcgcttcc agccgtggat gcccttccag
    13681 cgcaaagcgt ctaccgagta catcctttgc cagaccgtcg atggcaagcg gaataaactg
    13741 ccgcgaatcg taacgtagca acgcagacgc atcgcactcc agcacctgac gtagcgtggt
    13801 gatcaggcgc tgaaaacgat cctgatgacc aatcccacgc tgcaattcga tggcgatatt
    13861 cgccagcaca tcaacggaaa aactcatctt tgcctcactg tcaatttgac tatagatatt
    13921 gtcatatcga ccatttgatt gatagtcatt ttgactactc attaatgagc ataattttat
    13981 ttatagagta aaaacaatca gataaaaaac tggcacgtaa tctgcaatta gcaagacatc
    14041 tttttagaac acgctgaata aattgaggtt gctatgtcta ttgtggtgaa aaataacatt
    14101 cattgggttg gtcaacgtga ctgggaagtg cgtgattttc acggtacgga atataaaacg
    14161 ctacgcggca gcagttacaa cagctacctc atccgcgaag aaaaaaacgt gctgatcgac
    14221 accgtcgacc ataaattcag ccgcgaattt gtgcagaacc tgcgtaatga aatcgatctg
    14281 gcggatatcg attacatcgt gattaaccat gcagaagagg accacgctgg ggcgctgacc
    14341 gaactgatgg cgcaaattcc cgatacgccg atttactgta ctgccaacgc tatcgactcg
    14401 ataaatggtc atcatcatca tccggagtgg aattttaatg tggtgaaaac tggtgacacg
    14461 ctggatatcg gcaacggcaa acagctcatt tttgtcgaaa cgccaatgct gcactggccg
    14521 gacagcatga tgacttacct gacaggcgac gcggtgctgt tcagtaacga tgctttcggt
    14581 cagcactact gcgacgagca tctgttcaac gatgaagtgg atcagacgga gcttttcgag
    14641 cagtgccagc gttactacgc caatatcctg acgccgttca gccgcctggt aacaccgaaa
    14701 attaccgaga tcctgggctt taacttacca gtcgatatga tagcgacctc tcacggcgtg
    14761 gtatggcgcg ataacccgac gcaaattgtc gagctgtacc tgaaatgggc ggctgattat
    14821 caggaagaca gaatcaccat tttctacgac accatgtcga ataacacccg catgatggct
    14881 gacgctatcg cccaggggat tgcggaaacc gacccacgcg tggcggtgaa aattttcaac
    14941 gtcgcccgaa gcgataaaaa cgaaatcctg accaatgtct tccgctcaaa aggcgtgctg
    15001 gtcggcactt cgacgatgaa taacgtgatg atgccgaaaa tcgccggtct ggtggaggag
    15061 atgaccggat tacgcttccg taacaaacgc gccagtgctt tcggctctca cggctggagc
    15121 ggcggtgcgg tggatcgtct ttccacgcgc ctgcaggatg cgggtttcga aatgtcgctt
    15181 agcctgaaag ccaaatggcg accagaccag gacgctctgg agttatgccg tgaacacggt
    15241 cgcgaaatcg cccgtcagtg ggcgctcgcg ccgctgccgc agagcacggt gaatacggtg
    15301 gttaaagaag aaacctctgc caccacgaag tccgacctcg gcccacggat gcagtgcagc
    15361 gtctgccagt ggatttacga tccggcaaaa ggcgagccaa tgcaggacgt tgcgccagga
    15421 acgccgtgga gtgaagttcc ggataacttc ctctgcccgg aatgctctct cggcaaagac
    15481 gtctttgacg aactggcatc ggaggcaaaa tgagcaacgg cattgtgatc attggttcgg
    15541 gcttcgccgc ccgccaactg gtgaaaaata ttcgcaaaca ggacgccagt attccattaa
    15601 ccctgatcgc cgccgacagc atggatgagt acaacaaacc ggacctcagc catgttatca
    15661 gccaggggca acgtgccgac gaccttaccc gccagacggc gggtgaattt gccgggcagt
    15721 ttaatctgca cctgtttccg cacacctggg tgacggatat cgatgccgaa gcccatgtgg
    15781 tgaaaagcca gaataatcag tggcaatacg acaagctggt gctggcaact ggtgccagtg
    15841 cctttgtccc gccagtgcct gggcgtgagt taatgctgac gttaaatagt cagcaagagt
    15901 atcgcgcctg tgaaacgcaa ctgcgggatg cccgacgcgt gttgattgtc ggcggtggtt
    15961 tgattggtag cgaactggcg atggattttt gtcgtgcagg caaagcggtc acgctaatcg
    16021 acaacgctgc cagtattctg gcgtcattaa tgccaccaga agtaagcagc cgcttgcagc
    16081 atcggttgac ggagatgggc gttcatctgc tgttgaaatc tcagttgcag gggctggaaa
    16141 aaacggattc tggcattctg gcaacgctgg accaccagcg tagtatcgaa gtggatgcgg
    16201 taattgccgc caccggactg cgcccggaaa cagccctggc acgacgcgcc gggctgacga
    16261 ttaatcgcgg cgtttgcgtc gatagttatc tgcaaaccag taatgacgat atttatgcgc
    16321 tgggcgattg cgcggaaatc aacggtcagg tactgccatt cctgcagccg attcaactta
    16381 gcgcgatggt gctggcaaaa aatcttctcg gcaataacac gccgctgaaa ctaccggcga
    16441 tgctggtgaa aatcaaaacg ccggaattac cgctgcatct ggcaggcgaa acccagcgtc
    16501 aggatttacg ctggcaaatt aataccgaac gccagggaat ggtggctcgc ggtgttgacg
    16561 atgctgacca gcttcgcgcc tttgtggtca gtgaggatcg gatgaaagag gcatttggat
    16621 tgttgaaaac attgccgatg taggtgggct actgtgccta aaatgtcgga tgcgacgctg
    16681 gcgcgtctta tccgacctac ggggacgcat gtgtaggccg gataaggcgt ttacgccgca
    16741 tccggcaatg gtgtccaaat gcaacacgtt ttatccgttc tggacttcac ccgctaacca
    16801 acgcgccgca gcaataaccc cctgccccag agacaaaccg ccatcacccg ccggtaaact
    16861 ctgtggaaag agcaatgtga aatcagcgag ataatgcgcc agacgtgcac gcagcaaacg
    16921 gttatgaata accccgccgc taaataccag cgtagtgata ccacgcatcg tggcctgctc
    16981 acgcatcaac gcggcaaaac cctgcgccag cgcatcatga aacgcccacg cgcgttgatt
    17041 aaccggtgcc tgccagttca gccactgctg ccagaaagcg gcgagatcca gttgattgtc
    17101 caccagcggc atcgtcaccg gatgcgtcac tccgtggcat gaggctgcga gcgcctccag
    17161 agcacaagcc gcttcacctt cataacttaa cgtggctggc gcacagccca gtgccgccgc
    17221 cactgcatcg aacaaccgcc cacacgatga cgccagcggc gcgttaattc cacgctcaat
    17281 ggcccgcgcc agcacgctcc agttttgctg ttgcacactt gctgtttcag ggtaattctg
    17341 ccactccggc acaaagcgca ggcactgcgc cagcaggttt cgccacggct gcttcgctgc
    17401 caaatcgcca cccggaagcg ccactgcagg caagccgccc aggtgctcac attcgcgata
    17461 gttcacccgc aggcactcgc cgccccacaa agcgccgttc tcccccatac cgataccgtc
    17521 gagcgtcaaa gcaatgacat caccgccatc caacggccac tgatgctctg ccagacacgc
    17581 cgctgcatgg gcatgatgat gcagtaccgt ttgcgtcggc agattcattt cacgcgccca
    17641 ctggctggag acatagcccg gatgcgcgtc atgcacaacg tattgcgggg taaaatcgta
    17701 gatgttttgc atcaggcgta acgcttcgcg ccactgcatc tggatgccat cgtcacttaa
    17761 atcgcccaga tgctgactca acaccgcttg ttcaccgcgc accaggcaga aggtattttt
    17821 cagatccgcg ccgagacaca gcacaggcgg aacattttta aagcccggag gcaaagccag
    17881 cgcatccggc acataccccc gcgaacggcg cagcatttcg ccgctttcgc gcaccaccga
    17941 atcatccatc cgctgcacga tgtcgcggtt atgtatcaag aatccgtcgg caatgccctg
    18001 caaatccgcc agcgcctgtt cgttgctgat agctggtggt ttaccgctca ggttgccgga
    18061 ggtcatcacc agcgggcatt gcagttcctg taacagcaaa tgctggagcg ggttcgcagg
    18121 caacattacc ccgacttcgt taaggtcagg ggcgatatca tcacaaagct caggaacgta
    18181 ttttttatcc accagcacaa tcggcgcggc gggcgtggta agcaactggc gcgcagcgtc
    18241 tggtaaaccg tcagccactg gcaacatgac cgccagcggt ttcgccgggc gatgtttgcg
    18301 cgcccgaagt gtcgccaccg cgttactgtt acgtgcatcg caggcaagat gaaatccgcc
    18361 aatccctttg atggcgacaa tgttgcccat ttttaactgt gcgatagccg cctgtaatgc
    18421 cgcctcttgt tctgcatgtt caccatgact tacccattca agatgtgggc cacactccgg
    18481 gcaggccacc ggctgggcgt ggaagcgacg atcgagcggg tcacggtact ctttgtcaca
    18541 agccggacac aacggaaacg ccgccatcac ggtaaacggg cggtcgtaag gcatggcgcg
    18601 aataatggtg aaacgcgggc cgcagtgggt acagttgata aacggataac gataacgccg
    18661 ttcgcctggg gtattcattt cggcaaggca agcagggcaa gtagcggcat ccgggacaat
    18721 ttgcgtattc atggcacctc ccgcgctctg gcggatggtg aactcagtgg gcagttgtga
    18781 ccagatgtac ggctcacgct cgacgctatc aatacgcgcc agcggcgggc agtgctgatg
    18841 caattgaaca agtaacgttt ccgggtcttc ccgcagccgg acttctacgc catcgccgtc
    18901 attacagaca tcgccgtgaa gatttaattg ctgtgccagc tgccagacaa acggacgaaa
    18961 accgacgccc tgcactttgc cacgaatacg cagttggaca ccgcaagatg tgttttttgc
    19021 cattgagtta ttcccgccat catgaattgc gtaacccgtc ctgccggaca cgactgcgtc
    19081 gtatccggca gtctcaggtc ggcgataccg ccagctccgt attctacgaa tatttccggg
    19141 aattcctttg atgccagaac agttctgtaa gatttttaga acatcagcgc cgtacggcgg
    19201 cgtttttctg cactcagttg ttcaagttta ttacgatcga cacaaatcag cgcatgagtc
    19261 gggcaagccg ccatacacgc cgggccgtct tcacgatggt tgcacaggtc acatttattg
    19321 gcttcggctt tgtcagcccg tacattcagc cccgcgccgc tgttgcgaat caccggacgt
    19381 accaccactt ccatcgcgcc atacgggcaa gccacaacgc aggttttgca accaatgcaa
    19441 cgttcctgca tcacatgaac aaacccttta tcacggctga tagcaccatt cgggcagacg
    19501 ttagcgcacg gtgcatcttc acactgacgg caaactgtcg ccgtggaaat gttcacacct
    19561 ttaatgacat ggatacgcgg taaaaatgtt tccggggtca gcgatgcaca gtcctgattt
    19621 tcctgatgag aaaccacgca cgccacttca caggtacggc aaccaataca tttactcgcg
    19681 tcagcaatga tgaaacggtt catcaaattc tccagcaatg acagttaatg cgccgataca
    19741 ttcacaaatc atgccagttt taattcactg ttatttaagg aaattaattt ctgtaatgca
    19801 ggaaaaaaga tgtcatcgac actagtgacg atgacatgtg atgacaatgt ttatcgcgaa
    19861 agagcaatga gtgagtcgcg gcggatcagt tttccgctga aggttttcgg cggtgagaaa
    19921 tccccgccat cgagcataaa aatcagccgt ccaataattt cctgaatcat ctcagtcacc
    19981 ggaattttta cgctggagag cgccggaacg atataggggg caattgcgat atcatcgaat
    20041 ccgataactg acacctgctc tggcaccgct acgccgcgct cgtgcaacgc tttcatcgca
    20101 cctatcgcca tatcgtcgtt actggcaact aacgcgctaa atttagcccc acgttcgagc
    20161 aacgtttcta ccccttcggc cccgctggcg ggcgtccatt taccgttagc gataagtttt
    20221 tcattgagcg caataccatg ctgcgccagc gcgtctttat acccagcaag acgttcaatg
    20281 ctggtggggg aatccatcga gccggtaagg aaagcaatct cctgatgtcc ggcgtttatc
    20341 aactctgcca cggcattaaa gctggtctgt ttatgatcgc accagacgct atggctgctg
    20401 tttttgcgca ggcggcgatt aagcaccatt atcggctgac tgtgcgcgtc aatgatgtca
    20461 tcgatctcat ccacgcttaa aaaacgcggg taaatcataa tggcgtcgca gcgcaaatcc
    20521 agcagatact gaatcgcctg gcgctcttct tcggcgctgt gtttaccatc tgccaatagc
    20581 aactgccgcc ctttctcttc cgccattcgc gcggcatgaa agagtaattc actaaaataa
    20641 atgccgtggt aaagcgtgtt ggtcactacc aaccccagcg tctgagtact cttcgccgac
    20701 agattgcgcg ccagcaagtt tgggcggtaa ccgctctctt ctaccgcctg aaacacgcga
    20761 tctttagtct cctggctgac gtagccatta cctgaaagca cgcgggaaac ggtcgctttt
    20821 gaaaccccgg cgcgcttcgc cacttccagc atcgtcgtca tcattttcat ccctttacac
    20881 gcaatcaacg cagtgtactg caccgtttgc cgattgtcct tgcgcaatcg gcgggaaaaa
    20941 tattcaggtg accggtttca caaatataaa aaatgaacaa ttaactctct tgcttattta
    21001 gtgacaacta ttcatgattt tgtgaaaccg gtttcctaat tccgtttcag catcggcatt
    21061 tttccgttac gtcgactgat aacaactaca tctaccctac tgataacagg ataaaatccg
    21121 atggccaaaa attatgcggc gctggcacgc tcggtgatag cggcactggg cggcgttgat
    21181 aacatctcgg cggtcacgca ctgtatgacg cgtttgcgcg ttgttatcaa agatgaccag
    21241 cttatcgaca gcccgacgtt aaaaaccatc cccggcgtgc tcggcgtggt acgtagtgac
    21301 aaccagtgtc aggtgattat cggcaatacc gtttcacaag cctttcagga agtcgtcagc
    21361 ctgctgccgg gagatatgca gcccgcacag cccgtgggta aacccaaact cacgctgcgt
    21421 cgcattggtg cggggatcct cgatgcgctg atcggcacca tgtcaccgct gatcccggcg
    21481 attatcggcg gatcgatggt caaactgctg gcgatgatcc tcgagatgag cggcgtgctg
    21541 acaaaaggat cgccgacctt aaccattctg aatgtgattg gtgacggtgc tttcttcttc
    21601 ctgccgctga tggtcgcggc atctgccgcc atcaaattta aaaccaatat gtcgctggcg
    21661 attgccatcg cgggtgtgct ggtacatccg agttttattg aactgatggc gaaagcggcc
    21721 cagggtgaac atgtcgaatt tgccctgatt ccggtcaccg cggtgaaata cacctacaca
    21781 gtgatcccgg cgctggtgat gacctggtgc ctgtcatata tcgaacgctg ggtggatcgc
    21841 attacaccgg cggtgaccaa aaacttcctc aagccaatgc tgattgtgtt gattgccgca
    21901 ccgctggcaa tcctgctgat tggcccgatt ggtatctgga tcggtagcgc catttcggcg
    21961 ctggtttaca ccattcatgg ttatctgggc tggctttcag tcgccattat gggcgcgctg
    22021 tggcctctgc tggtaatgac cgggatgcac cgcgtcttta cgccaaccat cattcagacc
    22081 attgccgaaa ccggcaaaga agggatggtc atgccgtcag agatcggcgc taacctgtcg
    22141 ctgggcggtt catcactggc ggtggcgtgg aaaacgaaaa acccggaact gcgccagacg
    22201 gcgctggctg cggcggcatc agccattatg gcggggattt ccgaaccggc gttatacggc
    22261 gtggcgatcc gcctgaaacg tccgcttatc gccagtctta tcagcggttt tatttgcggc
    22321 gcggttgccg gtatggcggg gcttgccagc cactcaatgg cagcgccagg gttatttacc
    22381 agcgtgcaat tcttcgatcc ggcgaatcca atgagcatcg tctgggtgtt cgcggtcatg
    22441 gcgctggcgg tggtgctgtc gtttatcctc acactgttgc tcggctttga ggatattcct
    22501 gttgaggaag cggctgccga ggcgcgaaag catcagagcg tacaaccgac cgtcgccaaa
    22561 gaagtaagtc ttaattgagg atgaaaatgt cagtatttcc agaaggtttt ttatggggcg
    22621 gcgcgcttgc cgccaaccag tctgaaggtg cgttccgtga aggtggcaaa gggctgacca
    22681 ctgtcgatat gatcccacac ggcgagcatc gaatggcggt gaaactgggg ctggaaaaac
    22741 gttttcagtt gcgcgatgac gagttttatc ccagccatga ggcgacggat ttttatcatc
    22801 gttataaaga agatatcgcc ctgatggcag agatgggatt caaggttttc cgtacctcaa
    22861 ttgcctggag ccgtctcttt ccgcagggcg atgaaatcac gcccaatcag cagggcattg
    22921 ctttttatcg ttctgtcttt gaagagtgta aaaagtacgg tatcgaaccg ctggtcacgt
    22981 tgtgccactt cgatgtgccg atgcatctgg tcaccgaata tggctcctgg cgtaaccgca
    23041 agctggtgga gtttttcagc cgctacgccc ggacctgctt tgaagcattt gatggtctgg
    23101 tgaaatactg gcttaccttc aatgaaatca acattatgtt gcatagcccg ttctccggcg
    23161 cgggtctggt gtttgaagaa ggtgaaaatc aggatcaggt gaaatatcag gccgcgcatc
    23221 accagctggt tgccagtgcg ctagccacca aaatcgccca tgaggttaac ccgcaaaatc
    23281 aggtgggctg tatgctggcg ggcggtaact tctaccctta cagttgcaag ccggaagatg
    23341 tctgggcggc gctggagaaa gaccgggaaa acctgttttt tatcgatgtg caggcgcggg
    23401 gcacgtatcc ggcttactct gcccgcgtat tccgcgaaaa aggggtaacc atcgacaaag
    23461 caccgggcga tgatgaaatc ctgaaaaaca ccgtcgattt tgtctctttc agctattacg
    23521 cctcgcgctg cgcctcggcg gagatgaacg ccaacaatag cagtgcggcg aacgtagtga
    23581 aatcgctgcg taacccgtat ctgcaagtga gcgactgggg ctggggtatt gatccactcg
    23641 gtctgcgtat caccatgaac atgatgtacg accgttatca gaagccgctg tttctggtgg
    23701 aaaacggcct cggcgcaaaa gatgaacttg ctgccaatgg cgagattaac gatgactatc
    23761 gcatcagcta tttacgcgaa catatccgcg caatgggcga agcgattgca gatggcattc
    23821 cgctgatggg ctacaccacc tggggctgta ttgatttagt ctccgcctct acgggtgaaa
    23881 tgagcaaacg ctacggtttt gtctacgtag accgtgacga cgcaggcaac ggcacgctga
    23941 cgcgcacacg taagaaatcg ttctggtggt ataaaaaagt gattgccagt aatggggaag
    24001 atttagagta ggtaacagtg ccggatgcgg cgtgaacgcc ttatccggcc tacatgtcgc
    24061 acgaaccagg aaggcccgat aagcgcagcg catcgggcaa tctggggttt ggcatcagtc
    24121 aaaatcaaag aaaccattcc ggcttaacgc caaatcgggc tgaaagtgcc ttaatgtggg
    24181 atatcgtcag ggatcgttgt ccagacaaaa tctgactgac cagggattta gagccgattt
    24241 cgttcttcaa atccgcatag gtcagattat gttgatcaat cagcgtgcga agtaacgcca
    24301 cgccaacagg cattgccgca acggctttgt cgaactctgc aaactcttgg ttgttgtttt
    24361 catattctgc aatgcggctc gccagaaagt cgataagtgg attggtgtca tcttcttcaa
    24421 tcaaatattc gaccagttcc agtgcttcac gataatcatc ttctgagtca ctacctccca
    24481 gaaaggggac tgcattgacc agttctcttg ttgctttaac agcgcgtgca gcattagcag
    24541 tcattctttg ttctctctgt agtaacgtgt cagtttgtca tactcggcgt gattagttat
    24601 gtgcttcaca aagaaccgct tattaacaaa gtttatgtag gcaatcaccc ttaggttgtt
    24661 cccacctaca tccagaacgt accatttgtt gcgatattta aaattgtcga ggttgggaaa
    24721 cgccgttcgc atctcttctg gcgtagaaaa atccgtttcc ttaataaccc gatacaatgc
    24781 ctgaagagcc agagcgtcgt taggatattt acgcgcagac tcttcaaatg gcgctttaga
    24841 aatgatgtgc atttttaagt tttaacttcc ttatagtaag taaacagcca catctgcgag
    24901 ttctatcctt atgtgttcgg aaactatagc tcatcaaatc caagttcaca atatgtaaac
    24961 acttgagcgc gatataatta cccccaaaat cctcatagcc actctgcaaa aagtcagttc
    25021 tggttcaccc catcaagaac atcccccttt atcaagcaac gctgcgtctg cgccagatct
    25081 tccgtttgcg ggtcgcgccc ggcgatgggt ttgaggacga gtttacggtt gggggtataa
    25141 gtaaaacgcg tgagcgcggg atagcttaac ttgccaaacc agccgtgaac acgctgccat
    25201 ttggcgtctt ttacctgata cagactggtt atccagtaac cggaatcaaa ctgcatatcc
    25261 agcaattgcg tgtgaccatt gccttgcaaa tccagcaaat catctacgcc agttttgctt
    25321 gcggtgtaga atcccctggg ttcaaagaca cagggacggc cttcgcgcgt gaacgtcata
    25381 agaattaaat gtgcataagg agccagacca ttaccggtac ttggtaacca gataaccaga
    25441 tcctgaatgc cgttacgatc aagatcggcg cgatagagtt gccctttgcc cgctatgttt
    25501 gccagcatac ccacaggcac agaccaggat ttaccggcgc gatctttacc gttcaattgc
    25561 cattcactat catcatttgg catcttttca agcgtcacgc cagcaacgcc cacttttcta
    25621 aaatgccagg tttgctcggc atcataaggc atggtcagga gaggtttcag cgcccgcttt
    25681 tgcgcgtatt cgctgtcact cattggcaga tattcggtaa agctggtggc ctgtgcaggc
    25741 agcactaagc tcaataaaaa agcgcacatc cctgttctga tattcatttc gctgaattcc
    25801 ctgatggaga attattcttc ttccaccgct aactgcgcga agccgccatt cccttcccag
    25861 ccttccagtc gttgataaac ggtttctacc gcatctttaa tcggctgagt catcgggtag
    25921 taaaagccga cgatatccgg ctggatgccg aggaaaatca cttcgccaat gtcttctttc
    25981 aactggtcga taagatagtt cagcggcatg ttatgggtag tcatcataaa catctcggcg
    26041 atatcatccg ggtcgatgat gcggatttcg ccggggttta gccccatatc agtagcgtca
    26101 acaatcagta accgcgtagg gcgcagttca cggatggcga cgatgtcgtt ttccggtgca
    26161 ctaccgccat caatcaccac ccagttacct ttcggcgcgg cggcgcactt ttccgccagc
    26221 agcgggccag cgccatcatc gcccatcatg ctattgccaa cacagagtaa aacgtcagtc
    26281 acgcagtctc ctcaccatca aatagatggc gttttcctga tgaatatcat gcagcatact
    26341 taacagcatt gtactccatt ccttctgctg cgaagtttgc gctgcccggg ctttatcgaa
    26401 tgcgttggcc agcatcggca catggttaat gtcgatgacg atctcaccat atttcggcac
    26461 gccttccatt ttgcggcggg cttcactgcc cgcctccaga gtggcaatcc atgccagata
    26521 ttcatcccac gggcaggtga gcgccgcttc caggcaatcg ataaccccaa ggtggtgacc
    26581 aatcgccagg ctgtaataga ccacctgctg cgcctcggcg ggcgtggcat cgttctcatc
    26641 aataaattta cggctcagtt gactgaacac caccttttca ctcatcggat acgcgcctct
    26701 tcaacaacat gattcagatg gctgacaatc tcgttcagac gcggatcgtt ttccgcttcc
    26761 agccagcgtg caacctgttc ttcgccctgc cctaactgtg tcaggaagtc atcggcaatc
    26821 tgacgaccgt aacgataacc cgccaggcgg cgtgcttcgc gatccacttt cacacgcagc
    26881 ggctgcacca tatcgccatg caggatttcc gcctgttgtt catcctgctc acccgggcca
    26941 cgggcgtgaa ttttctgctc cagcaggccg agcgccattg caaagccgta cagcgtggcg
    27001 gcaggcgttg gcgggcaacc aggaatgtag acatctaccg ggacgatttt atccgtaccg
    27061 ccccacacgc agtagagatc gtggaaaata ccaccgctgt taccgcaggc accgtaggag
    27121 atacaaattt tcgggtccgg cgcggactgc cacgcacgca gcgcagggga tcgcattgca
    27181 cgggtgaccg cgccggtaaa cagtaaaata tccgcatgac gcggtgaagg aacgacttta
    27241 atgccgaagc gttctgcatc aaacagcggc gaaagcgtgg cgaaaatttc gatttcgcag
    27301 ccgttgcagc cgccgcagtc cacgcgataa acgtaggcag aacgtttgat ttttttcagt
    27361 aacgacgcct tcatgctggc gatggattca tccaccgtca tcgggaccgg aatgccgttg
    27421 gcgtcacggg ggcctaataa attgctcatc agatggcctc tttcatatgg cgagtcagtt
    27481 caatacggtc ggacggcacc aggcatttct ggcgcttaca ttccgggcaa gtctcaaagc
    27541 tttcgcggtg gttttccgcg cggctgtcgc cgttgtgctt aagcagcgca atggcgtagt
    27601 cgatctcttt ctggacggcg aaaggacgat tgcagacgcg gcagttgcac agcgcgaagc
    27661 gggactgttg caggaagtct tctttcttcc acactgccag ttcgtactct tgcgacagtt
    27721 tgatcgccgc cgtcgggcaa acttcttcgc agcgtccaca gaagatgcag cgcccgagat
    27781 taaactccca cgccagctca ccggtggcga ggtcagtttc aaccgttaag gcgtttgacg
    27841 ggcaggcatt gacgcaggcc gcacagccga tgcactgctg cgggttctgc tccggcttac
    27901 cacggaagtt tttatcaacc gcaatcggct ccagcggata agacgaggtc gccgtgccgg
    27961 ttttgatgac ttttttgata aaggtaaaca tggcgattcc ttatttcagc ggcgagtttt
    28021 tacgctcaat gctgtaacgc tcgagttctt tgtacggcac cactttgctc tttttcttac
    28081 gcacatcgac cacggtcatg cggtcggtac aggagtagca agggtccaag ctaccgataa
    28141 tcagcggcgc atcggaaacg gtgttaccgc gcagcatgta gcgcagggtc ggccagttcg
    28201 cgtaggtcgc ggcacggcag cgccagcggt acagcttctg gttgtcgccg gtcatgctcc
    28261 agtggatatc gtcgccgcgc ggcgcttcgg caaagcccag cgcgaaacgg tgcggaatgt
    28321 aggtaaagcc ttccaccatc agcggaccgc ccggcaggtt atccagaccg tagtcgatca
    28381 tgttcagcgc ggtataaact tcgttgatac gcactttcag acgggaaata acgtcgcagc
    28441 cctgctcgct gtggacttcc attggcagca ggccataacc gacaaacggg tgatcggcgc
    28501 gagtatcgcg ggcgtgaccg ctggcgcgaa ccatcgggcc aacgttactg aaatcgcggg
    28561 ccatttccgg gtccagacga ccaatgccga cggtgcgctg ttccatgttc ggcgtactca
    28621 gcagcacatc caccagctcc tgcacttcac gacgcatctg ttgcgccagc tggcgggtct
    28681 ggatcatgtc atctttcaac agatcgcgac gaatcccgcc gatcaggttc aggccgtagg
    28741 ttttacgcgc accggtgagg atctctgcca ttttcatgga ggtttcacgc acgcggaaga
    28801 actgcataaa gccggagtca aagccggtaa agtggcaggc gagaccgagg ttgagcagat
    28861 gcgagtgcag gcgttccacc tccagcagaa tggcgcggat catctgcgca cgttctggca
    28921 cctgaatacc catcgcgttt tctaccgacg tggtgtaggc ggtgctgtgg gcaaagccgc
    28981 agatcccgca cacacggtca gagaggaagg tcacttcgtt gtaacccata cgggtttccg
    29041 ccagtttttc catgccgcga tgaacgtaga acagacggta gtcggcgtcg ataatgtttt
    29101 cgccatcgac gaacagacgg aagtggcccg gttcgtcaga agtgacgtgc agcggaccaa
    29161 tcggcacgac gttgtttttc ttgtcgccca gttcgttgat gaactcgtag gtttcagcat
    29221 cggtggtcgg tgccggacgc tgacgataat ccatgctgtc tttacgcagc ggataaagtt
    29281 catccggcag caccagacgg cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    29341 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    29401 ccggtttgtt ggcatcaact tcgacgcgaa ccgttaccca gcacttagtc cccttctcca
    29461 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaaa
    29521 gcaccgacaa ccagcccccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    29581 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    29641 ccggaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    29701 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    29761 cccagtccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    29821 cagcgcgata accaggacgc cgacgaccag tttgacgatg gcaatcacca gcgccagcag
    29881 cagcccaccg gcggtgaagg tttccatctg tccccacggg ataaatacgc cgacgaacat
    29941 ctgcagcacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctgcc
    30001 gctgtattca gagagcgggc cttcctgcag ctcctgctcg gcttccgcca ggtcgaacgg
    30061 cagtttgccc atttcgataa aggtggcgaa cgcacaggca caaagcgcca gtaccagcgg
    30121 gatgctctgg ctcagcggcc agtgataaac ggtgtcggtg atgttgctga tgttggtgga
    30181 accggcaacc tgtgcggcaa cccacagacc aagcagcagc atcggttcga ccagcacgcc
    30241 aagcatcgct tcacggctcg cgccgatagc ggtaaacggg ctaccggtat ccagaccaga
    30301 aatggcaaag aagaaacgcg cgatggcaaa gagatacagt aaggtgatta aatcacccag
    30361 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    30421 gcccaccatc acatacggcg tcaggcggaa cacccagccg gaggcatccg ggccaacgct
    30481 ctggcggccc agcagtttga aaatgtcacg atactcctgc aacacacccg gcccacgacg
    30541 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    30601 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    30661 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    30721 ccttcgcact gccagcccgg caccagcgac accggattca gccatttgcg cagtttcagc
    30781 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    30841 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    30901 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    30961 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    31021 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    31081 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    31141 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    31201 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    31261 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    31321 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    31381 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    31441 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    31501 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccgttac gccagcgccc
    31561 agccccagca ggataatgcc gatattttcc agggtgtggt aagccagcag gcgctggatg
    31621 ttgtgttcca tcaacgcata cagaccaccg acgaacgcgg tgatcatgcc gagcaccagc
    31681 agcgcgatcc cccaccacag cggtgcatta ccgcccagca gtgacagggt taacatgccc
    31741 agcaggccaa ttttcatgac taccgtagaa aacaacgcgg cagctggcgc agaggcgtta
    31801 gcatgtgcct gcggcaccca gccgtgcagc ggaataatcc cggccagcag gccaaagcca
    31861 atcacaccga gcagccagat atcggaaccg agcggcagct gttgcatacg catatccagt
    31921 aggcacagat ccagcgtgcc ataacgctgc cacagcagcc agcaagcaat caccagcagc
    31981 agagtgccaa gacgccccag cgcaaaccac agtttgccct ctttgctgtt gctggtgagg
    32041 aacaccgcac acaaggccat gacttcggcc attaccacga acatgccgag gttgctggcg
    32101 atgacggcgc agacggcggc agccatcaac atattgatct gcaagccgtt gcacttcacc
    32161 tgcgcgtggc gatgccagtc aatgttgtag aggctgacaa acagaccgca cagaccgagc
    32221 gtaatcagcc aaatcgcgtt aagcggagag atttgcacat cgtagcttac cagcgacagc
    32281 gcaccgctca cgccaaccgc gccagtcagt acagtgaagc ccgcggctgc cgtatacaga
    32341 ctaccaaccg cgccgccaat tccagctatc cagccactta acgctttttg aaaagaaaag
    32401 agaaatgcca gaacagcggc ggcgacaaac catgccacgc cgctattgat cagggaaatt
    32461 gcgctcattt agcctctcca ctttgagcct gctgaaacaa ggtgagatcg ccaaagtccg
    32521 tgttaaaggt cagctcacgc ttacgtttgc tgacgcgggc gatatcggtg ttatccacca
    32581 gatgcagggc tttagtcggg cacatccgca cgcaggccgg accttgttca tcaaaactgc
    32641 aaaggtcaca tttgacggcg atcgcgcgaa tacccggcac ccagtcaagc agcgtactga
    32701 tacgcgccgg agcaggcggt gccggtggcg ctttcggggt attggcgttt gccggaatat
    32761 ccagcggacg gctgccggaa aattcaattg cgccaaacgg gcaggcgatg ccgcacagtt
    32821 tgcagcttac gcacagactt tcattcaact gcacggcccc atcaatgcgg gtgatggcgt
    32881 taaccgggca aaccaccgca cagggtgcat cttcacagtg gtgacagagc tgcggcgcag
    32941 attctttttc attcagcatc actctcaggc gcggcattga ttgcaggccg tgctggcgat
    33001 gcgtctctga acaggcggcc tcacaagtgt ggcagccgat acagagcgtg gagtcaacaa
    33061 ttacaaaacg attcaccagg cattcctcag gtgattgtca tttttgacga aaacatgccg
    33121 atgaaatgtc attttcgaca ctcatcgaca cgcccatccc caaacaggcg taacgcctgc
    33181 aaaacgggca aagcctcagc tcatgctgcc gggctttgtc cctttaccag ttggcttaaa
    33241 ttcaccggca cattgttttc aatagcggtg tataagctgt cgtaaacgcc agcgattttt
    33301 tcgagatagt gttccagaac ctgttcgcga tcgcggatgt tgaccttgcc atcaatcatg
    33361 ccgtcaatgc tcagttgcgc ctgcgcaatc cgctgtttgt cttcgccatc ttttgtttcg
    33421 acgtaatact cgatggtgtg gctgttaaag ccatccgcca gggtgacgta aatacgaaaa
    33481 tgttcaaaaa ggacgaaaca gagttctttg tccggcgcgc agctcatggc gtgatgcagg
    33541 cgcgctttcg ataacgtgat cccctgaacc agcgaattgc agtagatgtg ccactggtcc
    33601 tgtaggcgac gatgccgctg tgcgatgtaa tcggctttct cgcttatttc ccaaatagtc
    33661 attgtcaggt tacccgttta acagagatgc cagctttaag cattttctat gccaactttt
    33721 aatttattgt tattaaatag atttttaagc taaagatgaa tttcgtcgcc gtgtcgacgt
    33781 gtcatttcga catcatcgac attattcacc gcagggataa tcaacactgg cacaattatt
    33841 gcttgtagct ggcaatagtt aatgggaggc gatatgcacg aaataaccct ctgccaacgg
    33901 gcactggaat tgatcgaaca gcaggccgca aaacacggcg caaaacgcgt aactggggtc
    33961 tggctcaaaa ttggcgcatt ttcttgtgtc gaaaccagct ctcttgcctt ttgttttgat
    34021 ctggtttgcc gcggcagcgt ggcggaaggt tgtaaactgc acctcgaaga acaagaggcc
    34081 gaatgctggt gtgaaacatg ccaacagtat gtgacgctac tgacccagcg cgtccgccgc
    34141 tgtccacagt gtcatggtga catgctacag attgtggcag acgacggttt acagattcgg
    34201 cggatagaaa tagaccagga gtgagcgatg tgtacaacat gcggttgcgg tgaaggcaac
    34261 ctgtatatcg agggtgatga acataaccct cattccgcgt ttcgtagcgc gccatttgcc
    34321 ccggcggcac gcccgaagat gaaaatcacc ggcattaaag cgcctgaatt tacccccagc
    34381 cagactgaag aaggcgacct gcattacggt catggcgaag cgggcactca cgcgccgggc
    34441 atgagccagc gtcggatgct ggaagtcgaa attgacgtgc tggacaaaaa taaccgtctg
    34501 gctgaacgca accgcgcgcg ctttgctgcc cgcaagcaac tggtgctcaa cctggtttcc
    34561 agccctggtt ccggtaaaac caccctgctg acggaaaccc taatgcgcct gaaagacagc
    34621 gttccgtgcg cggttattga aggtgaccag caaaccgtga acgatgccgc acgcattcgc
    34681 gctaccggca caccagcgat tcaggtgaac accggtaaag gctgccatct tgacgcacag
    34741 atgattgccg acgccgcacc gcgtctgcca ctggacgata acggtattct gtttatcgaa
    34801 aacgttggca acctcgtatg cccggccagc ttcgatctcg gtgaaaaaca caaagtggcg
    34861 gtgctttccg ttaccgaagg tgaagacaaa ccgctgaaat atccgcatat gtttgccgcc
    34921 gcctcgctga tgctgctcaa caaagttgac ctgttgccgt atctcaactt tgacgttgag
    34981 aagtgcatcg cctgcgcccg cgaagtcaat ccagaaattg aaatcatcct tatttccgcc
    35041 accagcggcg aagggatgga ccagtggctg aactggctgg agacacagcg atgtgcatag
    35101 gcgttcccgg ccagatccgc accattgacg gcaaccaggc aaaagtcgac gtctgcggca
    35161 ttcagcgcga tgtcgattta acgttagtcg gcagctgcga tgaaaacggt cagccgcgcg
    35221 tgggccagtg ggtactggta cacgttggct ttgccatgag cgtaattaat gaagccgaag
    35281 cacgcgacac tctcgacgcc ttacaaaaca tgtttgacgt tgagccggat gtcggcgcgc
    35341 tgttgtatgg cgaggaaaaa taatgcgttt tgttgatgaa tatcgcgcgc cggaacaggt
    35401 gatgcagtta attgagcatc tgcgcgaacg tgcttcacat ctctcttaca ccgccgaacg
    35461 ccctctgcgg attatggaag tgtgcggtgg tcatacccac gccattttta aattcggcct
    35521 cgaccagtta ctgcctgaaa acgttgagtt tatccacggt ccgggttgcc cggtgtgcgt
    35581 actgccgatg ggtagaatcg acacctgcgt ggagattgcc agccatccgg aagtcatctt
    35641 ctgtaccttt ggcgacgcca tgcgcgtgcc ggggaaacag ggatcgctgt tgcaggcaaa
    35701 agcacgcggt gccgatgtgc gcatcgttta ctcgccgatg gatgcgttga aactggcgca
    35761 ggagaatcca acccgtaaag tcgtattctt cggcttaggt tttgaaacca ccatgccgac
    35821 caccgccatc accctgcaac aggcgaaagc ccgcgatgtg cagaattttt acttcttctg
    35881 ccagcatatt acgcttatcc cgacgctgcg cagtttgctg gaacagccgg ataacggtat
    35941 cgacgcgttc ctcgcgccgg gccacgttag tatggttatc ggcactgatg cctataattt
    36001 tatcgccagc gattttcatc gtccgctggt ggtcgctggt ttcgaacccc ttgatctact
    36061 gcaaggcgtg gtcatgctgg tggagcagaa aatagcggcc cacagcaagg tagagaatca
    36121 gtatcgtcgg gtggtaccgg atgccggtaa cctgctggcg caacaggcaa ttgctgatgt
    36181 gttctgtgtc aacggcgaca gcgaatggcg cggcttaggc gtgattgaat cttctggcgt
    36241 gcacctgacg ccggattatc aacgattcga tgccgaagca catttccgcc cagcaccgca
    36301 gcaggtctgc gatgacccgc gcgcgcgttg tggtgaggta ttaacgggca aatgtaagcc
    36361 gcatcaatgc ccgctgtttg gtaacacctg taatcctcaa accgcatttg gtgcactgat
    36421 ggtctcctcc gaaggagcgt gcgccgcgtg gtatcagtat cgtcagcagg agaatgaagc
    36481 gtgaataata tccaactcgc ccacggcagc ggcggccagg cgatgcagca attaatcaac
    36541 agcctgttta tggaagcctt tgccaacccg tggctggcag agcaggaaga ccaggcacgt
    36601 cttgatctgg cgcagctggt agcagaaggc gaccgtctgg cgttctccac cgacagttac
    36661 gtcattgacc cgctgttctt tcctggcggt aatatcggca agctggcgat ttgcggcaca
    36721 gccaatgacg ttgcggtcag tggcgctatt ccgcgctatc tctcctgtgg ctttatcctc
    36781 gaagaaggat tgccgatgga gacactgaaa gccgtagtga ccagcatggc agaaaccgcg
    36841 cgcgcggcag gcattgccat cgttactggc gatactaaag tggtgcagcg cggcgcggca
    36901 gataaactgt ttatcaacac cgctggcatg ggcgcaattc cggcgaatat tcactggggc
    36961 gcacagacgc taaccgcagg cgatgtatta ctggttagcg gcacgctcgg ggaccacggg
    37021 gcgactatcc ttaacctgcg tgagcagctg gggctggatg gcgaactggt cagcgactgc
    37081 gcggtactga cgccgcttat tcagacgctg cgtgacattc ccggcgtgaa agcgctgcgt
    37141 gatgccaccc gtggtggtgt gaacgcggta gttcatgagt tcgcggcagc ctgcggttgt
    37201 ggtattgaaa tttcagaagt ggcactgccg gttaaacctg ccgtgcgcgg cgtttgcgaa
    37261 ctgctgggac tggacgccct taactttgcc aacgaaggca aactggtgat cgccgttgaa
    37321 cgcaacgcgg cagagcaagt gctggcagcg ttacattccc atccactggg gaaagacgcg
    37381 gcgctaattg gtgaagtggt ggaacgtaaa ggtgttcgtc ttgccggtct gtatggtgtg
    37441 aaacgaaccc tcgatttacc acacgccgaa cctcttccgc gtatatgcta ataaaattct
    37501 aaatctccta tagttagtca atgacctttt gcaccgcttt gcggtgcttt cctggaagaa
    37561 caaaatgtca tatacaccga tgagtgatct cggacagcaa gggttgttcg acatcactcg
    37621 gacactattg cagcagcccg atctggcctc gctgtgtgag gctctttcgc aactggtaaa
    37681 gcgttctgcg ctcgccgaca acgcggctat tgtgttgtgg caagcgcaga ctcaacgtgc
    37741 gtcttattac gcatcgcgtg aaaaagacac cccattaaat atgaagacga aactgttctg
    37801 gcacacggtc cggtacgccg cattttgtcg cgccctgata cgctgcattg cagttacgaa
    37861 gaattttgtg aaacctggcc gcagctggtc gcaggtgggc tataccccaa atttggtcac
    37921 tattgcctga tgccactggc ggcggaaggg catatttttg gtggctgtga atttattcgt
    37981 tatgacgatc gtccctggag cgaaaaagag ttcaatcgtc tgcaaacatt tacgcagatc
    38041 gtttctgtcg tcaccgaaca aatccagagc cgcgtcgtca acaatgtcga ctatgagttg
    38101 ttatgccggg agcgcgataa cttccgcatc ctggttgcca ttaccaacgc ggtgctttcc
    38161 cgcctggata tggacgaact ggtcagcgaa gtcgccaaag aaatccatta ctatttcgac
    38221 attgacgata tcagtatcgt cttacgcagc caccgtaaaa acaaactcaa catctactcc
    38281 actcactatc ttgataaaca acatcccgcc cacgaacaga gcgaagtcga tgaagccgga
    38341 accctcaccg aacgcgtgtt caaaagtaaa gagatgctgc tgatcaatct ccacgagcgg
    38401 gacgatttag ccccctatga acgcatgttg ttcgacacct agggcaacca gattcaaacc
    38461 ttgtgcctgt taccgctgat gtctggcgac accatgctgg gcgtgctgaa actggcgcaa
    38521 tgcgaagaga aagtgtttac cactaccaat ctgaatttgc tgcgccagat tgccgaacgt
    38581 gtggcaatcg ctgtcgataa cgccctcgcc tatcaggaaa tccatcgtct gaaagaacgg
    38641 ctggttgatg aaaacctcgc cctgaccgag cagctcaaca atgttgatag tgaatttggc
    38701 gagattattg gccgcagcga agccatgtac agcgtgctta aacaagttga aatggtggcg
    38761 caaagtgaca gtaccgtgct gatcctcggt gaaactggca cgggtaaaga gctgattgcc
    38821 cgcgcgatcc ataatctcag tgggcgtaat aatcgccgca tggtcaaaat gaactgcgcg
    38881 gcgatgcctg ccggattgct ggaaagcgat ctgtttggtc atgagcgtgg ggcttttacc
    38941 ggtgccagcg cccagcgcat tggtcgtttt gaactggcgg ataaaagctc tctgttcctc
    39001 gacgaagtgg gcgatatgcc actggagtta cagccgaagt tgctgcgtgt attgcaggaa
    39061 caggagtttg aacgtctcgg cagcaacaaa atcattcaga cggacgtacg tttaattgcc
    39121 gcgactaacc gcgatctgaa aaaaatggtc gccgaccgag agttccatag cgatctctat
    39181 taccgcctga acgtattccc gattcacctg ccgccactac gcgagcgtcc ggaagatatt
    39241 ccgctgctgg cgaaagcctt taccttcaaa attgcccgtc gtctggggcg caatatcgac
    39301 agcattcctg ccgagacgct gcgcaccttg agcaacatgg agtggccggg taacgtacgc
    39361 gaactggaaa acgtcattga gcgcgcggta ttgctaacac gcggtaacgt gctgcagctg
    39421 tcattgccag atattgtttt accggaacct gaaacgccgc ctgccgcaac ggttgtcgcc
    39481 caggagggcg aagatgaata tcagttgatt gtgcgcgtgc tgaaagaaac caacggcgtg
    39541 gttgccgggc ctaaaggcgc tgcgcaacgt ctggggctga aacgcacgac cctgctgtca
    39601 cggatgaagc ggctgggaat tgataaatcg gcattgattt aactgcaaat tgccggacag
    39661 atctgcctgt ccggcatact attcacgagg ttttttcgga cgatattttt ccggcagttc
    39721 tggtaccgga cgcttgtcat cgatgagatg acgcacggtt aagatcggat gacgccacag
    39781 cattctcggc ccggcccaac gcataatctg tttcatctct tcacgctttg caggctggta
    39841 acagtgcacc ggacactgct tacaggctgg tttctcttcg ccgaacacac atttatccag
    39901 ccgcttttgc gcgtaaacaa acaacgcctc gtaatgctcc ggctccgctg acgcctgcgg
    39961 gcatttcgct tgataaagat cgatcatttt tttaatcgtc agtttttcac gagagatacg
    40021 cttgccggac atgctgcctc catactctta aggtgtattt atattacaac ttaatttgaa
    40081 aggggactat ggctctgaag gaggaggttt attcaaccga cgcaattttg cgtacttgct
    40141 tcataagcat cacgcaaaag ctgcaaaaca gcatctttcc cggaaccagc atcaagaact
    40201 cgccgttcgc ttcttcccct gaaatgatta actccggtat catgtgcgcc ttatgtgatt
    40261 acaacgaaaa taaaaaccat cacaccccat ttaatatcag ggaaccggac ataaccccat
    40321 gagtgcaata gaaaatttcg acgcccatac gcccatgatg cagcagtatc tcaggctgaa
    40381 agcccagcat cccgagatcc tgctgtttta ccggatgggt gatttttatg aactgtttta
    40441 tgacgacgca aaacgcgcgt cgcaactgct ggatatttca ctgaccaaac gcggtgcttc
    40501 ggcgggagag ccgatcccga tggcggggat tccctaccat gcggtggaaa actatctcgc
    40561 caaactggtg aatcagggag agtccgttgc catctgcgaa caaattggcg atccggcgac
    40621 cagcaaaggt ccggttgagc gcaaagttgt gcgtatcgtt acgccaggca ccatcagcga
    40681 tgaagccctg ttacaggagc gtcaggacaa cctgctggcg gctatctggc aggacagcaa
    40741 aggtttcggc tacgcgacgc tggatatcag ctccgggcgt tttcgcctga gcgaaccggc
    40801 cgaccgcgaa acgatggcgg cagagctgca acgcactaat ccggcggaac tgctgtatgc
    40861 agaagatttc gccgagatgt cgctgattga aggccgtcgc ggcctgcgcc gtcgcccgct
    40921 gtgggagttt gaaatcgaca ccgcgcgcca gcagttgaat ctgcaatttg gcacccgcga
    40981 tctggtcggt tttggcgtcg agaacgcgcc gcgcggactt tgtgctgccg gttgtctgtt
    41041 gcagtatgcg aaagataccc aacgcacgac cctgccgcat attcgttcta tcaccatgga
    41101 acgtgagcag gacagcatca ttatggatgc cgcgacacgt cgtaacctgg aaattactca
    41161 gaacctggcg ggcggtgcgg aaaatacgct ggcttctgtg ctcgactgca ccgtcacgcc
    41221 gatgggtagt cgtatgctga aacgctggct gcatatgcca gtgcgcgata cccgcgtgtt
    41281 gcttgagcgc cagcaaacta ttggcgcatt gcaggatttc accgccgagt tgcagccggt
    41341 actgcgtcag gtcggcgacc tggaacgtat tctggcacgt ctggctttac gaactgctcg
    41401 cccacgcgat ctggcccgta tgcgccacgc tttccagcaa ctgccggagc tgcgtgcgca
    41461 gttagaaact gtcgatagtg caccggtaca ggcgctacgt gagaagatgg gcgagtttgc
    41521 cgagctgcgc gatctgctgg agcgagcaat catcgacaca ccgccggtgc tggtacgcga
    41581 cggtggtgtt atcgcatcgg gctataacga agagctggat gagtggcgcg cgctggctga
    41641 cggcgcgacc gattatctgg agcgtctgga agtccgcgag cgtgaacgta ccggcctgga
    41701 cacgctgaaa gttggcttta atgcggtgca cggctactac attcaaatca gccgtgggca
    41761 aagccatctg gcacccatca actacatgcg tcgccagacg ctgaaaaacg ccgagcgcta
    41821 catcattcca gagctaaaag agtacgaaga taaagttctc acctcaaaag gcaaagcact
    41881 ggcactggaa aaacagcttt atgaagagtt gttcgacctg ctgttgccgc atctggaagc
    41941 gttgcaacag agcgcgagcg cgctggcgga actcgacgtg ctggtgaacc tggcggaacg
    42001 ggcctatacc ctgaactaca cctgcccgac cttcattgat aaaccgggca ttcgcattac
    42061 cgaaggtcgc catccggtag ttgaacaagt actgaatgag ccatttatcg ctaacccgct
    42121 gaatctgtcg ccgcagcgcc gtatgttgat catcaccggt ccgaacatgg gcggtaaaag
    42181 tacctatatg cgccagaccg cactgattgc gctgatggcc tacatcggca gctatgtacc
    42241 ggcacaaaaa gtcgagattg gacctatcga tcgcatcttt acccgcgtag gcgcggcaga
    42301 tgacctggct tccgggcgct caacctttat ggtggagatg actgaaaccg ccaatatttt
    42361 acataacgcc accgaataca gtctggtgtt aatggatgag atcgggcgtg gaacgtccac
    42421 ctacgatggt ctgtcgctgg cgtgggcgtg cgcagaaaat ctggcgaata agattaaggc
    42481 attgacgctg tttgccaccc actatttcga gctgacccag ctaccggaga aaatggaagg
    42541 cgtcgccaac gtacatctcg atgcgctgga gcacggcgac actatcgcct ttatgcacag
    42601 cgtgcaggac ggcgcggcga gcaaaagcta cggtctggcg gttgctgctc tggcgggcgt
    42661 accgaaagag gttattaagc gcgcacggca aaaactgcgt gagctggaaa gcatttcgcc
    42721 gaacgccgcc gcgacgcaag tggatggtac gcagatgtct ttgctgtcag tgccagaaga
    42781 aacttcgcct gcggtcgaag cactggaaaa tctcgacccg gattcactga ctccgcgtca
    42841 ggcgctggag tggatttatc gcttgaagag tctggtgtaa taacaattcc cgatagtctt
    42901 ttgctatcgg gaatattaac gacaactgac gaatcaaata aaaataccct gtataatagg
    42961 aaagcttatt ttacagggta aaaccatgcc atctacacgc tatcaaaaaa tcaatgccca
    43021 tcactatcgc catatatggg tcgttggtga tattcatggt gaatatcagt tattacaatc
    43081 ccgttaacat caactctctt tttaccctga aaccgactta cttatttcca caggcgataa
    43141 tattgatcgt gggccgaaga gtcttaacgt gctgcgcctg ctaaaccaac cctggtttac
    43201 gtcggttaaa ggcaaccacg aagcgatggc gttggatgca ttcgaaactg gcgatggaaa
    43261 tatgtggctt gccagcggtg gtgactggtt tttcgattta aatgattcag agcaacaaga
    43321 ggcaatagat ctgttgctga aattccatca ccttccacat attattgaaa tcattaatga
    43381 caacataaaa tatgccatc
//
LOCUS       DAESHI010000024        42133 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.024, whole genome shotgun sequence.
ACCESSION   DAESHI010000024 DAESHI010000000
VERSION     DAESHI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42133
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.024"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            448..600
                     /gene="mokC"
                     /locus_tag="KW011_001353"
     CDS             448..600
                     /gene="mokC"
                     /locus_tag="KW011_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HBH7659734.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(704..1834)
                     /gene="dnaJ"
                     /locus_tag="KW011_001354"
     CDS             complement(704..1834)
                     /gene="dnaJ"
                     /locus_tag="KW011_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBH7659735.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1923..3839)
                     /gene="dnaK"
                     /locus_tag="KW011_001355"
     CDS             complement(1923..3839)
                     /gene="dnaK"
                     /locus_tag="KW011_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBH7659736.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4216..4620
                     /locus_tag="KW011_001356"
     CDS             4216..4620
                     /locus_tag="KW011_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HBH7659737.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4646..5359
                     /gene="msyB"
                     /locus_tag="KW011_001357"
     CDS             4646..5359
                     /gene="msyB"
                     /locus_tag="KW011_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HBH7659738.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            5508..6074
                     /gene="satP"
                     /locus_tag="KW011_001358"
     CDS             5508..6074
                     /gene="satP"
                     /locus_tag="KW011_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HBH7659739.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(6109..6696)
                     /gene="mog"
                     /locus_tag="KW011_001359"
     CDS             complement(6109..6696)
                     /gene="mog"
                     /locus_tag="KW011_001359"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HBH7659740.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6811..7764)
                     /gene="tal"
                     /locus_tag="KW011_001360"
     CDS             complement(6811..7764)
                     /gene="tal"
                     /locus_tag="KW011_001360"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBH7659741.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            8043..9472
                     /locus_tag="KW011_001361"
                     /pseudo
     CDS             8043..9472
                     /locus_tag="KW011_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            9542..10318
                     /gene="yaaA"
                     /locus_tag="KW011_001362"
     CDS             9542..10318
                     /gene="yaaA"
                     /locus_tag="KW011_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBH7659742.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10471..10767)
                     /locus_tag="KW011_001363"
     CDS             complement(10471..10767)
                     /locus_tag="KW011_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBH7659743.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10981..12267)
                     /gene="thrC"
                     /locus_tag="KW011_001364"
     CDS             complement(10981..12267)
                     /gene="thrC"
                     /locus_tag="KW011_001364"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBH7659744.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12268..13200)
                     /gene="thrB"
                     /locus_tag="KW011_001365"
     CDS             complement(12268..13200)
                     /gene="thrB"
                     /locus_tag="KW011_001365"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBH7659745.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(13202..15664)
                     /gene="thrA"
                     /locus_tag="KW011_001366"
     CDS             complement(13202..15664)
                     /gene="thrA"
                     /locus_tag="KW011_001366"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HBH7659746.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15691..15810)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15746..15811)
                     /gene="thrL"
                     /locus_tag="KW011_001367"
     CDS             complement(15746..15811)
                     /gene="thrL"
                     /locus_tag="KW011_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HBH7659747.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(16025..16711)
                     /locus_tag="KW011_001368"
     CDS             complement(16025..16711)
                     /locus_tag="KW011_001368"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBH7659748.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(17111..17251)
                     /locus_tag="KW011_001369"
     CDS             complement(17111..17251)
                     /locus_tag="KW011_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659749.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17347..18063
                     /gene="arcA"
                     /locus_tag="KW011_001370"
     CDS             17347..18063
                     /gene="arcA"
                     /locus_tag="KW011_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HBH7659750.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(18123..19475)
                     /gene="creD"
                     /locus_tag="KW011_001371"
     CDS             complement(18123..19475)
                     /gene="creD"
                     /locus_tag="KW011_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HBH7659751.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(19533..20957)
                     /gene="creC"
                     /locus_tag="KW011_001372"
     CDS             complement(19533..20957)
                     /gene="creC"
                     /locus_tag="KW011_001372"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HBH7659752.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20957..21646)
                     /gene="creB"
                     /locus_tag="KW011_001373"
     CDS             complement(20957..21646)
                     /gene="creB"
                     /locus_tag="KW011_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HBH7659753.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21659..22132)
                     /gene="creA"
                     /locus_tag="KW011_001374"
     CDS             complement(21659..22132)
                     /gene="creA"
                     /locus_tag="KW011_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HBH7659754.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22343..23212
                     /gene="robA"
                     /locus_tag="KW011_001375"
     CDS             22343..23212
                     /gene="robA"
                     /locus_tag="KW011_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HBH7659755.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(23209..23856)
                     /gene="gpmB"
                     /locus_tag="KW011_001376"
     CDS             complement(23209..23856)
                     /gene="gpmB"
                     /locus_tag="KW011_001376"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HBH7659756.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23908..24429
                     /gene="yjjX"
                     /locus_tag="KW011_001377"
     CDS             23908..24429
                     /gene="yjjX"
                     /locus_tag="KW011_001377"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HBH7659757.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(24514..24840)
                     /gene="trpR"
                     /locus_tag="KW011_001378"
     CDS             complement(24514..24840)
                     /gene="trpR"
                     /locus_tag="KW011_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HBH7659758.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24930..26867)
                     /gene="sltY"
                     /locus_tag="KW011_001379"
     CDS             complement(24930..26867)
                     /gene="sltY"
                     /locus_tag="KW011_001379"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HBH7659759.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            27078..28745
                     /gene="ettA"
                     /locus_tag="KW011_001380"
     CDS             27078..28745
                     /gene="ettA"
                     /locus_tag="KW011_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HBH7659760.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(29052..30284)
                     /gene="nadR"
                     /locus_tag="KW011_001381"
     CDS             complement(29052..30284)
                     /gene="nadR"
                     /locus_tag="KW011_001381"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HBH7659761.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30305..31687)
                     /gene="radA"
                     /locus_tag="KW011_001382"
     CDS             complement(30305..31687)
                     /gene="radA"
                     /locus_tag="KW011_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBH7659762.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31736..32704)
                     /gene="serB"
                     /locus_tag="KW011_001383"
     CDS             complement(31736..32704)
                     /gene="serB"
                     /locus_tag="KW011_001383"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HBH7659763.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32810..33454
                     /locus_tag="KW011_001384"
     CDS             32810..33454
                     /locus_tag="KW011_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HBH7659764.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            33482..34498
                     /gene="lplA"
                     /locus_tag="KW011_001385"
     CDS             33482..34498
                     /gene="lplA"
                     /locus_tag="KW011_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HBH7659765.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            34530..34679
                     /locus_tag="KW011_001386"
     CDS             34530..34679
                     /locus_tag="KW011_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659766.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
     gene            complement(34954..35673)
                     /gene="deoD"
                     /locus_tag="KW011_001387"
     CDS             complement(34954..35673)
                     /gene="deoD"
                     /locus_tag="KW011_001387"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBH7659767.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(35753..36976)
                     /gene="deoB"
                     /locus_tag="KW011_001388"
     CDS             complement(35753..36976)
                     /gene="deoB"
                     /locus_tag="KW011_001388"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBH7659768.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(37028..38350)
                     /gene="deoA"
                     /locus_tag="KW011_001389"
     CDS             complement(37028..38350)
                     /gene="deoA"
                     /locus_tag="KW011_001389"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HBH7659769.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(38477..39256)
                     /gene="deoC"
                     /locus_tag="KW011_001390"
     CDS             complement(38477..39256)
                     /gene="deoC"
                     /locus_tag="KW011_001390"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBH7659770.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            39514..41064
                     /locus_tag="KW011_001391"
     CDS             39514..41064
                     /locus_tag="KW011_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HBH7659771.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            41036..41899
                     /locus_tag="KW011_001392"
     CDS             41036..41899
                     /locus_tag="KW011_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HBH7659772.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
BASE COUNT         9945 a        11409 c        10590 g        10189 t
ORIGIN      
        1 acacggaaag tcatatcagc gctaacgggt gaccctaagc cctgataatc atagcttaag
       61 gtcaggattt ctgagggatt aattaggaaa agtaataatt aatttcttgt cagtcggcgt
      121 tatttgttag gacaatattt caaaaataat ccagcttgtc gtcaataatt gcgataaata
      181 gataaattaa agttaaataa ttaaatcatt aaaaaattac gccacaaaaa tgcgaggcgt
      241 cttcaggatt agaatcgata aaacagaatg ggttaaagag agcgatataa tagcggcggg
      301 tgcttgaggc tatctgtctc aggcattagc tgaacggcag atagagaaaa gccccgagtg
      361 atattttact atcaacccga ggccccctat atgctgaaca catgtagagt gcctcttact
      421 gaccgtaagg tcaaggagaa gagagcaatg aagcagcata aggcgatgat tgtcgccctg
      481 atcgtcatct gtatcaccgc cgtagtggcg gcgctggtaa cgagaaaaga cctctgtgag
      541 gttcacatcc gaactggcca gacggaggtt gctgttttca cggcttacga atccgagtaa
      601 gagcaacggc ggggagtgat ccccgccact tttcaggtgc tcgcatatct tcaacgcacc
      661 ctaattttac ccaagcctgc ccacgggcag gcttttgggg aggttagcgg gtcaggtcgt
      721 caaaaaactt cttcacacca tcaaagaagc tctttgagcg cgggctgttg tgctcaccgg
      781 ttgggccacc gaagctttct tgcagctctt gcagcaactg tttctgcttc tcgttcaggc
      841 ctaccggtgt ttcgacgaca acgcggcaca gcaaatcacc ctgtgcgcca ccgcggacag
      901 acttgacgcc tttaccgcgc atacggaaaa gcttaccggt ctgggtttcg ccaggcactt
      961 tcagtttgac gcgaccatca agggtcggta cttcgatttc gccacccagc gccgccatag
     1021 cgaagttgat cgggacttcg caatagaggt tgttgccttc acgctcgaaa atcgggtgct
     1081 gtttaacctg aacctgaacg tacagatcgc ctgccggtgc gccatgctcg cccgcttcac
     1141 cttcgcccgc aagacggatg cggtctccag tgtccacccc tgccgggatt ttaacggaca
     1201 gcgttttgct gcgctcaaca cgaccatgac catgacattt gttgcacgga tctttgatca
     1261 gcgtaccgcg gccctgacag tgtggacagg tctgctgtac agcgaagaat ccctggcgca
     1321 tctgcacctg accagaacca tgacaggtcg gacaggtctg cggctgtgta cctggttttg
     1381 caccgctacc gtggcaaacg tcacactctt ccagagtcgg aatgcggatc tctttggtca
     1441 cgccacgtac agcttcttcg agggtgagct ccatgttata gcgtaaatca gcaccgcgcg
     1501 ccgcacgttg acgaccacgt ccgccgccaa aaatatcgcc gaaaacgtca ccaaaaatat
     1561 cgctgaagtc tgcgccgccg ccaaaaccgc cgccgcccat gccaccttgc tcaaacgcag
     1621 catgaccata ctgatcgtat gccgcacgtt tttgcgagtc ggtcagaact tcataagctt
     1681 ccttgatctc tttaaatttc gcctcggcct ctttgtcacc ctggttacgg tccgggtggt
     1741 atttcatggc caggcgtttg taggcctttt tgatttcacg ctcttccgct gttttggaaa
     1801 cgcctaaaat ctcgtaataa tcttgcttag ccatcttttt taaattgccc ctagatgaat
     1861 gcacgggcgg agaggaaatt ccccttcgcc cgtgtcagta taattacccg tttatagggc
     1921 gattattttt tgtctttgac ttcttcaaat tcagcgtcga caacatcgtc atctttcgcg
     1981 ttgtttgcag aagcatcagc accggcagtc tgctgctggg catgttgctg ctgggcgatt
     2041 tccatcagtt tctgggaaac ctgtgccagt tcctgcattt tcgcttcgat agcggctttg
     2101 tcttcacctt tcagagcagt ttccagtgca gtcagcgcag actcgatagc agttttgtcg
     2161 tcagccggca gtttgtcgcc tgcttcttca acctgcttac gggtgctgtg cagcagatgg
     2221 tcgccctggt tgcgagtctg taccagctct tcaaacttac ggtcagcttc ggcgttagct
     2281 tctgcgtcgc gtaccatttt ctggatttca tcttcgttca gaccagaaga agccttgatg
     2341 gtgatcttct gctctttacc gctgttttta tctttcgcgg aaacgtgcaa gataccgtca
     2401 gcatcgatat cgaaggtaac ttcgatctgc ggcatgccgc gcggtgccgg gttgatacca
     2461 tccaggttga actgacccag agatttgtta tcagctgcac gtttacgttc accctgcagc
     2521 acatggatgg ttaccgcaga ctggttgtct tcagcggtag agaacacctg gctgtgcttg
     2581 gtcgggatag tggtattttt cgcgatcagc gtcgtcatca caccgcccat ggtttcgata
     2641 cccagagaca gcggggtaac gtccagcagc agtacgtctt ttacgtcacc agtcagaaca
     2701 ccaccctgaa cagcagcacc gattgctaca gcttcgtccg ggttaacgtc tttacgcggc
     2761 tctttaccaa agaactcagc aactttcttc tgaaccattg gcatacgagt ctgaccaccc
     2821 acgaggataa cgtcgtcgat atcagatacg gacaggccag cgtcctgcag tgcaactttc
     2881 agcggctcaa tggaacggtt taccagatct tcaaccaggc tttccagttt cgcacgagtc
     2941 actttgatgt tcatgtgttt cggaccggtc gcatctgcag tgatgtacgg caggttaacg
     3001 tcggtctgct gagcggaaga cagttcgatt ttcgcttttt ctgccgcttc tttcaggcgc
     3061 tgcattgcca gcggatcgtt gcgcaggtca atgccctgat ctttcttgaa ttcttcaacc
     3121 aggtagttga tcagacggct gtcgaagtct tcacccccca ggtgggtatc accgttggta
     3181 gccagaactt cgaaggtttt ttcgccgtca acttcgtcga tttcgataat agaaatatcg
     3241 aaagtaccac cacccaggtc ataaaccgcg atagtacggt tgccagtacc tttgtccaga
     3301 ccgtaagcca gcgcagctgc ggtcggttcg ttgatgatac gttttacttc cagaccagcg
     3361 atacggcctg cgtctttggt tgcctgacgc tgagcatcgt taaagtatgc cggtacggtg
     3421 ataacagctt cagttaccgg ttcacccaag taatcttcag cggttttctt catttttttc
     3481 agcacttcag cagaaatctg cggcggtgcc attttctggc ctttaacttc gacccatgcg
     3541 tcgccgttat cagcagcaat aattttgaac ggcatgatgg aaacatcacg ctgtacttct
     3601 tcgtcctgga agcggcggcc aatcaggcgt ttaatcgcaa acagggtgtt ttgcgggttc
     3661 gtcactgcct gacgtttagc cggctgacca accagagttt caccatcctg ggtataggca
     3721 ataatagaag gcgtggtgcg atcgccttcg gcgttctcca gcacgcgagg agtggtgcca
     3781 tccataatcg ctacacaaga gttggtagta cccaggtcga taccaattat tttacccatc
     3841 taaacgtctc cactatatat tcggtcatca tgtggttgtg agtctgtaat aggggcgaaa
     3901 cgtctggttt caactgccca atttcatttt tttgcagact cactcactgc ggttgactac
     3961 taaatggggt cgtaacccac gtcatcaagg gggagatgca aaaatttttt gtgcttttgc
     4021 gcagaaattt cgcgatatgg caggacaaga cgtaaaattg acagcaattt tattcatata
     4081 aagaatgaat cgcagccaag gcattcatca aaaaattgta ataaaaagaa aagattacgt
     4141 acctgaatct tctctttatc agcagtaaac tagtgggtat tcatccctct acctcttccc
     4201 actaagagaa tccttatgaa atccgttttt acgatttccg ccagcctggc gattagcctg
     4261 atgctgtgct gcacggcgca ggcaaacgac cataaactcc tcggcgtcat tgcaatgccg
     4321 cgtaacgaaa ccaacgatct ggcgctgaaa cttcctgttt gtcgcattgt gaaacgcata
     4381 caactctctg ccgaccatgg cgatttacag ttaagcggtg catcggttta tttcaaagcc
     4441 gcccgtagcg ccagtcagag tctgaatatt ccttcagaaa taaaagaagg gcaaaccact
     4501 gactggatca acattaacag tgataacgac aataaacgct gcgtctcaaa aatcaccttt
     4561 tccggtcata cggtgaactc atcggatatg gccacgctga aaattatcgg cgacgactaa
     4621 cgccagaata tagaaaccac aaaaaatgaa tgttaattac ctgaatgatt cagatctgga
     4681 ttttcttcag cattgtagtg aggaacagtt ggcaaatttc gcccgattgc tcacccataa
     4741 tgaaaaaggc aaaactcgcc tctccagcgt actgatgcgc aacgaactgt ttaaatcgat
     4801 ggaagggcat cccgagcaac atcgccgcaa ctggcagctg attgctggag aattacagca
     4861 ttttggtggc gatagtatcg ccaacaaact gcgcggacac ggtaaattgt atcgggccat
     4921 tttgctcgat gtttcaaaac gattgaagct gaaagccgac aaagagatgt ctacgtttga
     4981 gattgagcaa cagttactgg aacaatttct gcgtaacacc tggaagaata tggacgagga
     5041 acataagcag gagtttctgc acgcggtcga tgccagggtg aatgagctgg aagagctgct
     5101 gccgctgctg atgaaagaca aattattggc aaaaggcgtg tcgcatttgc tttccagcca
     5161 attgacccgc attttacgca cccacgcagc aatgagcgtg cttgggcatg gtttgctgcg
     5221 cggcgcgggt ctgggaggcc ctgtgggcgc agcattaaat ggggttaaag cggtcagcgg
     5281 cagtgcctat cgcgtgacaa ttccagccgt aatacaaatc gcctgcctgc gccggatggt
     5341 tagcgccact caggtctgac accgtaaacc ggggaaaatt ttcattttcc cctcaaaaga
     5401 tcgtagacac tgccccactg gctgattatt atgccgcgcc ctgaaaacac tactgttatt
     5461 cagggaaatt atttcaccat tcattcgatg atgatttttg aggaattatg ggcaacacta
     5521 agttggctaa tccggcaccg ctgggcctga tgggcttcgg catgaccacc attctgctta
     5581 acctgcacaa cgtgggttat ttcgctctgg acggtattat tcttgccatg ggcattttct
     5641 acggcggcat cgcgcaaatt tttgccggtc tgctggagta caaaaaaggc aacactttcg
     5701 gtttaaccgc attcacctct tacggttctt tctggttgac gctggttgcg attctgctga
     5761 tgccgaaact gggtctgacc gatgcgccaa atgcacagtt ccttggtgtc tacctgggtc
     5821 tgtggggcgt atttacgctg tttatgttct tcggcacgct gaaaggcgca cgcgttctgc
     5881 aattcgtttt ctttagcctg actgtgctgt ttgccctgct ggcgatcggt aacattgccg
     5941 gtaacgccgc aatcatccac tttgccggct ggattggtct gatctgtggt gccagtgcaa
     6001 tctatctggc gatgggtgaa gtactgaacg agcagtttgg tcgcaccgtt ctgccgattg
     6061 gtgaatccca ctaattgttt tgagatcccc ccgctcgggg ggattttttt attcgctaac
     6121 gtcgcgtctt gcactcttcg gtctgaatgc agcaaccact tctggtgccg tttcaacgta
     6181 tggcccttcc agcaactgaa tgcagtacgg tacgctggca aaaataccgt gtaccacaac
     6241 gttacccgca gcgtccttca caccttccag cgtctcttta atagacttcg gctgaccggg
     6301 taagttaagg atcagcgcct gtttgcgaat cacaccaacc tgacgcgaaa ggatcgcagt
     6361 tggtacaaaa tgcaggctta tctggcgcat ctgttcacca aagccaggca tctcgcggtc
     6421 cgctactgcc agcgtcgcat cgggcgttac gtcacgacgc gccgggccag ttccgcccgt
     6481 ggtgagcacc agatggcaac tcatttcatc cactagctca cacaacgttt gctcgatgat
     6541 cgcctgctca tcggggatta agcgggtttc cagttcaaac ggcgtggtta gcgccgatgt
     6601 cagccattct tccagcgcag ggatgccttt atcctgataa acgccgctgg atgcgcgatc
     6661 agagatggaa actaagccaa tacgtaaagt attcatcaag atttttccgt ttaaacagtc
     6721 tcgttaaaca gaatgataca ctgcgaaggg agtgacagac aggcttcaga agaggtagcg
     6781 tgaccgactt cccggtcacg ctaagaatga ttacagcaga tcgccgatca ttttttccag
     6841 tttttcctgg tcaatagcaa acttacggat accttccgcc agtttatcta ctgccattgg
     6901 atcctggttg tgctgccaca ggaactcgga ctcagtgata cgcgccggac gcgctttcac
     6961 ttcgccggtg taagacagtt tacgttcgat agccccttcg ctctcagcca gctctttcag
     7021 cagtgccggt gcgatggtaa gacggtcgca gcctgccagt tccagaattt cgccgatgtt
     7081 acggaagctt gcgcccataa ccacggtttc ataaccgtgc tctttgtagt actggtagat
     7141 ttcagataca gaaaccacgc ccggatcttc tgccggagcg tactctttct tatcagtatt
     7201 cgctttgtac cagtcaagaa tacggccaac aaatggcgag atcaggaaca cgcctgcttc
     7261 cgcacaagca cgagcctgag cgaaggagaa cagcagggtc aggttacagt tgatgccttc
     7321 tttttccagc tgttctgcag cacggatacc ctgccaggta gaagccagtt tgatcagaat
     7381 acgatcgttg ctaataccag catcgttgta gagtttgatc aggcgttttg ctttcgcaat
     7441 tgacgcttcg gtgtcatagg aaagacgcgc atcaacttca gttgagatac ggcccggaac
     7501 cagtttcagg atttccagac caatatttac tgccagtttg tcggtcgcgt ccacgatctg
     7561 ctgcgcgcga tcgttgctct gctgtttcgc ccaggcgaca gcatcatcaa tcaacttacg
     7621 gtattccgga atctgcgctg cgttaagaat gagagaaggg ttggttgtgg catcctgcgg
     7681 ttgatacagc ttcattgccg cgatgtcccc agtgtcggcc actacggtgg tgtactgacg
     7741 aagggaggtc aatttgtccg tcatgatagt atttctcttt aaacagcttg ttagggggat
     7801 gtaaccggtc tgccctgatg atatcacgac gcaacgtcag cgcaaccgcc gacaagacgg
     7861 ttatcgccag gcgagactgt ttcggatttc tgacactgcg ttgatatcgc ccgccatttt
     7921 tatacaaaac ctcatgtatg ctacgcagaa gttatcaagt acctcgtagc gtatatactt
     7981 cttaaacaat tggtaacgtt tacacaggaa agtcatcgcg accggcaata agagggatat
     8041 gcatgccaga ttttttctcc tttattaaca gcgtcctttg gggatcggta atgatttacc
     8101 tgctcttcgg cgcaggttgt tggttcactt ttcgcaccgg atttgtgcag tttcgctaca
     8161 tccgccagtt tggcaaaagt cttaaaaata gcattcatcc acagccaggc ggtttaacct
     8221 catttcagtc attgtgtacc agtcttgcgg cgcgcgtggg tagcggcaac ctggccggcg
     8281 ttgcgctggc tattaccgcc ggtggacctg gagccgtctt ttggatgtgg gttgccgcgt
     8341 ttatcggcat ggcgacctcg tttgccgaat gttcccttgc acaactttat aaagaacgtg
     8401 acgtcaatgg acagtttcgt ggaggaccgg catggtatat ggcgcgcggg ctggggatgc
     8461 gttggatggg cgttctgttc gccgtctttt tgctcatcgc ctacggcata attttcagcg
     8521 gagttcaggc gaacgccgtt gcccgagccc tgagttttct tttgattttc ccccgctggt
     8581 gacaggcatt attctcgctg tctttgctct gctggcaatc actcgcggtc ttcatggcgt
     8641 cgcccggctc atgcaggggt ttgtcccgtt gatggcgata atctgggtgc tgaccagcat
     8701 ggtgatttgc gtaataaata tcgggcaact tccccacgtc atttggtcta tttttgagag
     8761 tgcttttggc tggcaggaag cggcaggcgg cgcggcggga tataccttaa gccaggcgat
     8821 tactaacggt tttcagcgca gtatgttttc caatgaggcg ggaatgggtt cgacgccaaa
     8881 cgcggcagcg gcagcggcgt cctggcctcc gcatccggca gcgcaaggga ttgtccagat
     8941 gattggcatt tttatcgaca ccctggtcat ctgtacggca agcgccatgc tgatattact
     9001 ggcgggtaac gacacaacct acatgccgct ggaaggtatt cagcttatcc agaaggcgat
     9061 gcgggtgcta atgggttcct ggggtgctga gtttgttacc ctcgtggtta ttctgtttgc
     9121 cttcagctcc atcgttgcca actacattta tgccgaaaac aatctcttct ttttacgcct
     9181 gaacaaccct aaagcgatct ggtgtttgcg gatctgcacc ttcgcaacgg tcatcggcgg
     9241 caccttgcta agtcttccgc tgatgtggca actggcagat atcataatgg cctgcatggc
     9301 tattaccaat ttgaccgcca ttttactgct ctcgcctgtg gttcatacca ttgccagtga
     9361 ttatctacgc cagcgtaaac tcggcgtgcg cccggtgttt gatccgttgc gttatccgga
     9421 tatcggtcgc cagctttctc cggacgcgtg ggatgatgtt tcgcaggagt aatcacaact
     9481 atcgatcaac tcattctctt tttttgctaa agtcggcata aatttcctgc aaggactgga
     9541 tatgctgatt cttatttcac ctgcgaaaac gcttgattac caaagcccgt tgaccaccac
     9601 gcgctatacg ctgccggagc tgttagacaa ttcccagcac ttgatccatg aagcgcggaa
     9661 actgacgcct ccgcagatta gcacgctgat gcgcatcagc gacaaactgg cgggtatcaa
     9721 cgccgctcgc tttcatgact ggcagccaga tttcacgccg gagaatgccc gccaggctat
     9781 tttggcgttt aaaggtgatg tctacaccgg cttgcaggcc gaaactttca gcgaagacga
     9841 tttcgatttt gcccaacagc atttgcgaat gctttccggc ttgtatggcg tactgcgccc
     9901 gctcgattta atgcagcctt atcgtctgga aatggggatc cgtcttgaga atgcccgagg
     9961 gaaagatctg tatcaattct ggggagatat catcaccaac aagctgaacg aggcgctcgc
    10021 agcacaaggc gataatgtgg tgattaatct ggcgtcagat gagtatttta aatcggtgaa
    10081 gccgaagaaa ttgaatgccg agattatcaa gccggtgttc ctcgatgaga agaacggcaa
    10141 atttaagata atcagcttct acgctaagaa agcacgcggt ctgatgagtc gtttcattat
    10201 tgaaaatcgg ctgaccaaac cagagcaact gactggtttt aatagcgaag gttacttctt
    10261 tgatgaagct tcctccagca atggcgaact ggtgtttaaa cgctacgagc agcgttaagt
    10321 ttgcagtcaa tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag catgcagatt
    10381 caatatattg cagaattcac gtaggcctga taagacgcgc cagcgtcgca tcaggcgctg
    10441 aatgccggat tgttacccgg catttgtcat ttagcggtga tgtttgcctg gaccatgacc
    10501 gccgtgatga tcatgaggag ctttcttatg gtggcgcggc ggtggcggcg gtccgtgtgg
    10561 gtgccagcga ttgcctcgcc attcataatg ttgtttccac cagccgtggt cgcgccagtg
    10621 acctccatcc cagtaatagc cacgattatc acgatcgcct atctgtaatt ttactgacgg
    10681 gactaacgta atttccgcag cctgtgctgc catgggagcg accagaacca gggaaagtgc
    10741 gagtacgata gattgcatct ttttcacagg ttactccttt tatcattgcc cgttatgggt
    10801 cgatatccgg agattacgcg atgagaacga cggttgttat tctccgcaat cctaatctct
    10861 aagttaccgc atttattgag aatttctcct ttttccctgt cgtttttctc cataatttct
    10921 tcataaaaaa gccgggctgc ataaaagcaa acccggcctg attgagataa tgaatagatt
    10981 ttactgatga ttcatcatca atttacgcaa cgcagcaaaa tcggcgggca gattatgtga
    11041 aagcaagggt aaatcagcac gttctgccag ctcttttggc agatccaacg tttcaccgag
    11101 aatcgcttcc acgctctctt taaatttcgc cggatgcgcg gtgccgagga acaagccata
    11161 ttcgcctgga ttcaactggt cacacagcgc acgatacgct acggcagcgt gcggctccga
    11221 ggtgtagccc agttctttta actcacgcat tgtctgttgc gtggtttcat catccacggc
    11281 tgcataaccc agctctttca gttgccagat tttgcggcgg aacaactctt ccacacgcgg
    11341 ccagttgttc ggctgactca cgtccatcgc gttggataac gtcgcctgag tcgctttggg
    11401 tgaccactga ccgtcgtgca ggaaacgtgg cacggtatcg ttcacgttgg tcgcagcaat
    11461 aaaacgtttc accggcagac cgagtgactt cgccagcaga cccgccgtca aatcgccgaa
    11521 gtttccgctt ggcaccgaga caaccagctg gttgcgcgct tcctgcggca gctgcgcaac
    11581 tgcttcaaag tagtagcaaa tctgcgccag caaacggctg atgttaatcg agttagccga
    11641 gtttaacccc agcgccactt tcagttcttc atcatcaaac gcctgcttca ccagcgcctg
    11701 acaggcatcg aaatcgccgt cgatggcaac agtttcgata ttgccgccca acgtacagaa
    11761 cagtttttct tgcagtggac tgattttgcc tcgtggatag aggataacca ctttcacatt
    11821 cggcaaaccg tagaaagcat gagccactgc cgctccggta tcaccggagg tcgcggtcag
    11881 aatggtcact ggcttatcgc ccgcaatatg ggtcagcatt tgtgccataa agcgaccgcc
    11941 gaaatcttta aatgccagcg ttggcccgtg gaacaattcc agacaaccga catcgctttc
    12001 aacattggcg accggagccg ggaaggcaaa cgccgcgcgc acgcgctctt ccaggatttc
    12061 ctgcgggatt tcatcaccaa taaacgccga gaggatcttc gcactgcggg tgacaaaatc
    12121 cagcttcagc atctcatcaa tttcagtcag gctgaattcc ggcaggtcat gcggaaaaaa
    12181 cagcccctga tttttgccca acccctgggt tacggcttgc gcaaagctga cctgctcgtt
    12241 gtgatctttc agattgtaga gtttcattta gttttccagt actcgtgcgc ccgccgtatc
    12301 cagccggcaa atatgaacaa aaccttcctg attttgcagg tagttcttac ccaaccagtc
    12361 ggcaacgcgc tgggcggtat ccggcttgtc acatagagcg aacaaggtcg ggccggagcc
    12421 ggagataccg ctcgctaccg cgccgatttc cgcgaccgcc tgccgcgcct gccggaagcc
    12481 aggcagtaac cgttcacggt agggttcagc gataacatct ttcatcagct tcgcggcaag
    12541 ctcaggctga cgggaatagc aggcgtgaat gaagccagcc agatgtcgcc cgtgcgcaat
    12601 gcaatcctgg cggcgatact gcgccggtaa aatagcccgg gcttctgccg tcgagacttt
    12661 aattcccgga tacgccagca cccacagcca ctcatcaaac cctggcactt gctggctgat
    12721 gatgtcgttt tcttcgatca tcaactgcat accaccaaga aaacacggtg ccacgttgtc
    12781 gtaatgaatg ctgccggaga tacgtccttc cagctcgccc atcaaagcca gcaaacgggt
    12841 gtcattaagt ggcttgccgc agtgttcatt catcgccatc agcgccgcga ccaccgaaca
    12901 ggcgctggag cctaagcccg agccgatcgg catattcttt tccagagtca tcgccactgg
    12961 aatttgcttg cccagctcca ggcaaaaacg ctcccagcac tgataaacga tattttcccg
    13021 tggttctgac ggcagcttat cggcaaagcg tccgaggttg ttgagactga atgtctctgc
    13081 cgcctcaacc gtgactacat ctccgagcaa tgcaccatca acgggtgtca ccgccgcccc
    13141 gagcacatca aacccgacgc tcatattggc actggaagcc ggggcataaa ctttaaccat
    13201 gtcagactcc taacttccat gagagggtac gtagcagatc ggcaaagaca ccagcagcgg
    13261 taacgtcatt gcccgcacca tatccgcgca gcaccaacgg cagcggctga taatagtggc
    13321 tataaaaggc cagggcgttt tcgccatttt tcactttgaa cagcggatca ttcccatcca
    13381 cttcggcaat cttcacgcgg cagacaccat cttcatcaat attgccaaca tagcgcaaaa
    13441 cttttccttc atcacgggcc ttcgccacgc gcgcggcaaa gagatcgtcg agctgtgaca
    13501 gattcgccat aaaagcggca acatcaccct cggcgttaaa ctctgcgggc agcacaggtt
    13561 caatttcaat atccgccagc tccagttcac gtcccgtttc acgagcgaga atcaatagtt
    13621 tacgcgccac atccatacca gaaagatcat ctcgcgggtc cggttcggta taacccattt
    13681 cccgcgccag cgtggtcgcc tcggagaaac tcatgccttc gtctaacttg ccgaagatat
    13741 aagaaagcga acctgaaaga atgccggaga acttcaccaa ttcatcacct gcattgagca
    13801 gattttgcag gttctcaata accggtaatc cagccccaac gttggtgtca tagaggaatt
    13861 tacgccgcga tttttccgcc gcataacgca actgatgata gtaatccatc gacgaggtgt
    13921 tggccttttt gttcggcgtg acaacgtgga aaccttcgcg caggaagtcg gcatattgat
    13981 ccgccactgc ctggctggag gtgcagtcaa caatgaccgg gttcagcaga tgatattctt
    14041 tcacgaggcg aattaagcgc ccgagattaa acggctcttt ggcttgtgca agttcttcct
    14101 gccagttttc cagattaagg ccatgcacac tagtgagcag agccttcgag ttggcaacgc
    14161 cgcagacacg taagtcgata tgtttattct tcagccagct ttgctgacgc ttcagttgct
    14221 ccagcagcgc accgccaacg ccaccgacgc caatcacaaa cacttcgata acctgatcgg
    14281 tattgaacag catctgatga gtaacgcgca cgccagtggt cgcatcatcg ttatttacca
    14341 cgacagagat tgagcgttca gaagatccct gagcaatggc gacaatgttg atattggcgc
    14401 gggccagcgc ggcaaagaat ttcgccgaga tcccacgcaa ggtgcgcata ccatcaccta
    14461 ccaccgagat aatggccagc cgttccatca ccgccagcgg ctccagtaag ccttctttca
    14521 gttccaggta gaactcttcc tgcattgccc gttcagcccg cacacagtcg ctttgcggaa
    14581 cgcagaaact gatactgtat tcggaagatg attgcgtaat cagcaccacg gaaatacggg
    14641 cgcgtgacat cgcggcaaag acgcgcgccg ccatgccgac cattcctttc atccccgggc
    14701 cggaaacgct gaacattgcc atgttattca gattggaaat gcccttgacc ggtaattcgc
    14761 cttcatcacg actggcacca atgagcgtac ctggtgcttg aggatttccg gtatttttaa
    14821 tcaggcaagg gatctggaac tgggcgatgg gggtaatggt gcgggggtga agaactttag
    14881 cgccgaagta ggaaagctcc atcgcttcct ggtaggacat cgacttcaac aacctcgcat
    14941 cgggcacctg acgcgggtcg caggtataga ccccgtcaac gtccgtccaa atctcgcaac
    15001 aatcggcgcg taaacaggca gccagcaccg ccgcggagta gtcggaaccg ttgcgtccaa
    15061 gtaccaccag ttcgcctttt tcattaccgg cggtgaagcc tgccatcagc accatgtgat
    15121 cagccggaat acgacttgcc gcaatacggc gggtggactc agcaatatcg acggtagatt
    15181 cgaggtaatg ccccactgcc agtagttttt cgaccggatc gataacggta acgttgtgac
    15241 cacgcgcttc taacacgccg gccataatgg cgatcgacat tttctcgcca cggcaaatca
    15301 gcgcagcgtt aatgctatcc gggcactgcc ctaacaaact aatgccatgc aggacatgtt
    15361 ttatttgggc aaattcctgg tcgacgaaag ttttcaattg cgccagcggg aatcccggct
    15421 gggcggcggc gagtcccgtc aaaagttcgg caaaaatacg ttcggcatcg ctgatattgg
    15481 gtaaagcatc ctggccgcta atggtttttt caatcatcgc caccaggtgg ttggtgattt
    15541 tggcgggggc agagaggacg gtggccacct gcccctgcct ggcattgctt tccagaatat
    15601 cggcaacccg cagaaaacgt tctgcatttg ccactgatgt accgccgaac ttcaacactc
    15661 gcatggttgt tacctcgtta cctttggtcg aaaaaaaaag cccgcactgt caggtgcggg
    15721 cttttttctg tgtttcctgt acgcgtcagc ccgcaccgtt acctgtggta atggtgatgg
    15781 tggtggtaat ggtggtgcta atgcgtttca tggatgttgt gtactctgta atttttatct
    15841 gtctgtgcgc tatgcctata ttggttaaag tatttagtga cctaagtcaa taaaatttta
    15901 atttactcat ggcaggtaac cagttcagaa gctgctatca gacactcttt ttttaatcca
    15961 cacagagaca tattgcccgt tgcagtcaga atgaaaagct gaaaaatact tactaaggcg
    16021 ttttttattt ggtgatattt ttttcaatat catgcagcaa acggtgcaac attgccgtgt
    16081 ctcgttgctc taaaagcccc aggcgttgct gtaaccagtc gaccagtttt atgtcatctg
    16141 ccactgccag agtcgtcagc aatgccatga ctcgttcgcg taaagcttgc agttgatgtt
    16201 ggtctgccgt tgtgtcactt ttcgccggtt gttgtattaa tgttgctaat tgatagcaat
    16261 agaccatcac cgcctgcccc agattgagcg aaggataatc cgccaccatc ggcacaccag
    16321 taagaacgtc agccaacgct aactcttcgt tagtcagccc ggaatcttcg cgaccaaaca
    16381 ccagcgcggc atggctcatc catgaagatt tttcctctaa cagcggcacc agttctactg
    16441 gcgtggcgta gtaatgatat ttcgcccgac tgcgcgcagt ggtggcgaca gtgaaatcga
    16501 catcgtgtaa cgattcagcc agtgttggga aaactttaat attatcaata acatcaccag
    16561 atccatgtgc gacccagcgg gtggctggct ccaggtgtgc ctgactatcg acaatccgca
    16621 gttcgctaaa acccatcgtt ttcattgccc gcgccgctgc cccaatattt tctgctctgg
    16681 cgggtgcgac caggataatc gttatacgca tattgccact cttcttgacc aaataaccgc
    16741 gaacagggtg atcactgtca acttattacg cggtgcgaat ttacaaattc ttaacgtaag
    16801 tcgcagaaaa agccctttac ttagcttaaa aaaggctaaa ctatttcctg actgtactaa
    16861 cggtttaatt gttaaaaaat gctacatatc cttctgttta cttaggataa ttttataaaa
    16921 aataaatctc gacaattgga ttcaccacgt ttattagttg tatgatgcaa ctagttggat
    16981 tattaaaata atgtgacgaa agctagcatt tagatacgat gatttcatca aactgttaac
    17041 gtgctacaat tgaacttgat atatgtcaac gaagcgtagt tttattgggt gtccggcccc
    17101 tcttagcctg ttatgttgct gttaaaatgg ttaggatgac agccgttttt gacactgtcg
    17161 ggtcctgagg gaaagtaccc acgaccaagc taatgatgtt gttgacgttg atggaaagtg
    17221 catcaagaac gcaattacgt actttagtca tgttacgccg atcatgttaa tttgcagcat
    17281 gcatcaggca ggtcagggac ttttgtactt cctgtttcga tttagttggc aatttaggta
    17341 gcaaacatgc agaccccgca cattcttatc gttgaagacg agttggtaac acgcaacacg
    17401 ttgaaaagta ttttcgaagc ggaaggctat gatgttttcg aagcgacaga tggcgcggaa
    17461 atgcatcaga tcctctctga atatgacatc aacctggtga tcatggatat caatctgccg
    17521 ggtaagaacg gtcttctgtt agcgcgtgaa ctgcgcgagc aggcgaatgt tgcgttgatg
    17581 ttcctgactg gccgtgacaa cgaagtcgat aaaattctcg gcctcgaaat cggtgcagat
    17641 gactacatca ccaaaccgtt caacccgcgt gaactgacga ttcgtgcacg caacctgctg
    17701 tcccgtacca tgaatctggg tactgtcagc gaagaacgtc gtagcgttga aagctacaag
    17761 ttcaatggtt gggaactgga catcaacagc cgttcgttga tcggccctga tggcgagcag
    17821 tacaagctgc cgcgcagcga gttccgcgcc atgcttcact tctgtgaaaa cccaggcaaa
    17881 attcagtccc gtgctgaact gctgaagaaa atgaccggcc gtgagctgaa accacacgac
    17941 cgtactgtag acgtgacgat ccgccgtatt cgtaaacatt tcgaatctac gccggatacg
    18001 ccggaaatca tcgccaccat ccacggtgaa ggttatcgct tctgtggtga tctggaagat
    18061 taatcggctt taccaccgtc aaaaaaaacg gcgcttttta gcgccgtttt tatttttcaa
    18121 ccttatttcc agatacgtaa ttcatcgtcc gttgtaactt ctttactggc tttcattttc
    18181 ggcagtgaaa acgcatacca gtcgatatta cgagtcacaa acatcatgcc ggccagcgcc
    18241 accaccagca cactggttcc caacaacagc gcgctatcga cagagttgag cagtccccac
    18301 atcactccat ccagcaacaa cagcgcgagg gtaaacaaca tgctattgcg ccagcctttc
    18361 aataccgcct gcaaataaat accgttcatt aacgccccaa tcagactggc gattatccat
    18421 gccacggtaa aaccgatatg ttcagaaagc gccagcaaga gcaaataaaa catcaccaat
    18481 gaaagcccca ccagcaaata ttgcattggg tgtaaacgtt gcgcggtgag cgtttcaaaa
    18541 acaaagaacg ccataaaagt cagtgcaatc agcagaatgg cgtacttagt cgcccggtcc
    18601 gttaattggt attgatcggc tggcgtcgtc actgcgacgc taaacgcgag gaagttttcc
    18661 cagccggtat cattgcctga agcaaaacgc tcaccgagat tattagcaaa ccagctgctt
    18721 tgccattgcg cctgaaaacc tgactcgcta acttcccgtt tggctggcaa aaaatcacct
    18781 aaaaaactgg gatgcggcca gttgctggtt aaggtcattt cgctattacg cccggcaggc
    18841 accacagaaa gatcgccggt accgcttaaa ttcagggcca tattcagctt caggttctgc
    18901 ttccgccagt ccccttcggg taaagggata tgcacgccct gcccgccttg ctctaacccg
    18961 gtgccgggtt caatggtcag cgccgttccg ttaacttcag gcgctttcac cacaccaata
    19021 ccacgcgcat ccccgacgct aatcacaata aatggcttgc caaaggtgat atttggcgcg
    19081 tcgagttcgc taagacgcga aacatcgaaa tcggctttta gcgttaaatc actgtgccag
    19141 acctgaccgg tataaatccc tatcttgcgt tcttccacgt tctgattgcc atcaaccatc
    19201 aatgactcag gtaaccaaaa atggataaaa cttcgtttcc gctccacggt tttatcctct
    19261 tcctgtaccg tataaagctc ggtcacagga atagcgataa gcggcccaac aagtttttgc
    19321 ggcccgctgg tactttggcg aatcgcatct tccacatcgc tacggtaatc ggcacgttcg
    19381 acaatcacct gccgaatcag cattatcgga atcaacaaca gcaatactgc accaaacagg
    19441 gtggtcattt tccagaacag ggaggatttc aacatagtct tctcctttgc aatggcagca
    19501 ggatacgaag gctatgtggg aagaatttga agctatgtga agtgacggtg aagtcgaagc
    19561 gaggccagca cgccaccttc ctgcacgttg cgcagcgtga cttcgccgtt aaacaaacgg
    19621 gcgacctcac tgacgaacgc caaccccaga ccgctgcttt tttgcccatt tgcacgcggc
    19681 aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt caggaatacc actaccggta
    19741 tccagcacct taagcgtgac gtgttcctga tccacttcgg cgcttagcgt tatacgaccg
    19801 ctctcggggg taaaatcgat ggcgttatcc agtaaattcc ccagcgcctg atccagtaac
    19861 gccggttcag cagcaacatt aacctcggtg ggcgtaacat gtagagtgat gtttttttct
    19921 gccaactgca cggtgcgcgc ttcgctgacg cggcggaata atgccgccac atcaacagca
    19981 gtcaaaacga cttcctgacg attctccagt cttgcctggc gtagtaacgt ttccaccagt
    20041 gcctgcattc gcgcattttg cgtcagaatg ttgtcagtaa aacgagccac tacttcaggc
    20101 ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg ccgccagtgg gctttttagc
    20161 tcatgggtta acgcataaac atactgctca atatagtttt tcccttccag cttcacgcgc
    20221 atactttcca gcgcctgcgc gagtttacgc agctcgctac taccgagatc gggaagagga
    20281 acgggcttat tgtcagtgac ggaatcagca tagcgagtga gccgggcaat ggagcggttg
    20341 atccaccaaa ccatgcctgc gccaatcacc agtgcgatcc ccaacaaaat ggcgctggcc
    20401 cataaaattc gccgctcgct gcgtttaatg accggagcca tcgccgcgtt cggtttgcct
    20461 acgctcaaaa cgccaataag ccgcgagccg tccataatcg gcgcggcaac atacatcacc
    20521 gaactttcgg gatcggcagg attttgcaac gtgctacgcg caccatactg accacgcaac
    20581 gttagccaga cgtcattcca gcgcgaataa tcctgcccaa cggctttatt tgccgaatcg
    20641 aacaatactt tgccctgcgc atcggtcata tagacatgat attcattgcg cactttgtta
    20701 atgccaccga tattggcgcg aaacgggcga tgttgtagct gattaaacgc ctgcgccagt
    20761 tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg ccagctccgc cagcaacgtt
    20821 gcggtatcga ttaacgtccc ctccgttgct cttcgcacgc ccggtttaac ttctttgaca
    20881 aaaatagcca gtacgaacca ggccgccacc gccaccagta aaaaatagcc cagcagcaac
    20941 cgcatgccga tacgcattac aggcccctca ggctatatcc catgccgcga tgagtattaa
    21001 tcggagaaag atcggggttg atggcgcgca gcttggcacg caacgtttta atgtgggtat
    21061 cgacggtgcg atcgtaggtg tcctgcgcat cttcccacac gctatccatt agttgctggc
    21121 gggaccagac gcggcctggc gacttgagta acgtcttcag caataaaaac tcataccgcg
    21181 tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc gggttcattc aattcaaaat
    21241 gaccaatacg gatgacggga gacggcgacg agaacttctt cacccgacgc agtaaggtgc
    21301 gcaccctggc gcacacttcg cggggtgaaa acggtttagc cacatagtcg tcagcgccaa
    21361 tttccagccc aagcaggcga tcgacctctt cacttcgggc cgtcaggaac agtacaggta
    21421 acgccggatg gagcgccagt aactggcggc acaattcaaa gccgctgata tcaggcaaac
    21481 caacatcgag aatcatgacg tcgggaacct gctggcgagc tttatccagc accggcaagc
    21541 ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa catgtagacc agcgtgtcgg
    21601 ctatcccttg ctcatcttcc actaaccaga ccgtttcccg ttgcataaat cgcctctgtt
    21661 attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt tttcggcgaa ccttctacaa
    21721 ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt ggcgtcataa aagcgcacga
    21781 cctgtaacga cttaaagacc agggacgtgc gttttttgaa tactacctcg ccctgagctt
    21841 tgccgttttt aatacgatcc gacagttcaa tcggcccgac ttgctgacaa gaaatagccg
    21901 catcggaggt atcttccgcc agacccaatc cccctttaat accacctgtt ttcgcccggc
    21961 tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa ggcttccaca acaattttgt
    22021 gatccgggcc gatcatttta aatacggtgt cgaccgaacc aatctcttct gcatgagcca
    22081 atggccccag cattatcagg cttaaagaca ggatcaaatg cttgtatttc atattgttac
    22141 catttttatc tattacacga gcaattagtt cgtcacggta aataacatat tgattaagaa
    22201 caaaatctca atacttttat ttccgtcaag ccctaaaaca tactctacta agaaaaaaac
    22261 actgaatgct aaaacagcaa aaaatgctat tatccaatta cctgatgtca ggtgctcgtt
    22321 gttgaaagga tgaggatatt ttatggatca ggccggcatt attcgcgacc ttttaatctg
    22381 gctggaaggt catctggatc agcccctgtc gctcgacaat gtagcggcga aagcaggtta
    22441 ttccaagtgg cacttacaga gaatgtttaa agatgtcact ggccatgcta ttggcgcgta
    22501 tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta cgcctgactg cgcgtccgat
    22561 tctggacatc gcgctgcaat accgcttcga ctctcaacag acatttaccc gcgcattcaa
    22621 gaagcagttt gcccagactc ctgcacttta ccgccgttct cctgaatgga gcgcctttgg
    22681 tattcgcccg ccgctacgtc tgggtgaatt cacaatgcca gagcacaaat ttgtcaccct
    22741 ggaagatacg ccgctgattg gtgttaccca gagctactcc tgttcgctgg agcaaatctc
    22801 tgatttccgc catgaaatgc gttatcagtt ctggcacgat tttctcggca acgcgccgac
    22861 cattccgcca gtgctctacg gcctgaatga aacgcgtccg agtcaggata aagacgacga
    22921 acaagaggta ttctatacca ccgcgttagc ccaggatcag gcagatggct atgtactgac
    22981 ggggcatccg gtgatgctgc agggcggcga atatgtgatg tttacctatg aaggtctggg
    23041 aaccggcgtg caggagttta tcctgacggt atacggaacg tgcatgccaa tgctcaacct
    23101 gacgcgccgt aaaggtcagg atattgagcg atactacccg gcagaagacg ccaaagcggg
    23161 agatcgccca attaatctgc gctgtgaact gctgattcct atccgccgtt aacgctgcag
    23221 ctcatctaat gcaggggcgt ccagatgcga gatgtcccct gcggtttcaa cgacccagcc
    23281 agacgccagc cacaggcttt cctgataatc cacgcgcgaa atagaacagt tacgcagacg
    23341 taagcggcgc tctgcccatg ctggtaatcc gagaatcgta ctcaccagac atcccagtgc
    23401 aataccatgg ctcaccagca gcggtcggct tccctgcggt aagtcccggc aagattccag
    23461 tgccgcattg acgcgatcgc tgagctcctg cattgactcg ccttcaggaa tacgcccgtc
    23521 aacggtgcca ttgaccagct gccgacgcca gttctcttct tcttcggtca gagaatcgat
    23581 atgtcttttt tccagcacac ccatgtttaa ttcacgcagg cgagaatcaa agatgatgtc
    23641 acagccgcag gcctgggcga tgatttccgc cgtacgccgg gtgcgtccta aatcgctact
    23701 gataatatgc gtaatgccaa gctctttggc acgggttgcc acctgcatcg cctgttgctc
    23761 acctttggcg gtcagcgggc tgtcagactg gccctgaata cgtcgctcgg cgttccactg
    23821 cgtttcaccg tggcggacta ggtatacctg taacatgctt tttttccgta tactgctgta
    23881 acgttaattt tctgagtaat gctgattatg caccaagtta tctgtgcgac caccaatccc
    23941 gctaaaattc aggccattct gcaggcattt cacgagatct tcggcgaagg atcctgccat
    24001 attgcatccg ttgccgtcga gagcggtgta ccggaacagc cctttggcag tgaggaaacg
    24061 cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt tacttccaga ggctgatttt
    24121 tgggtggcga ttgaagctgg catcgatggt gacagcactt tcagttgggt ggtgattgaa
    24181 aacaccagcc agcgcggcga ggcgcgttcg gctactttgc cgttacctgc ggtgattctg
    24241 cagaaagtgc gtgaaggtga ggcgcttggt cccgtgatgt cgcgttatac cggtattgat
    24301 gagattggtc gtaaggaagg ggcgatcggc gtgtttaccg ccgggaaact cactcgcgcc
    24361 agcgtctatc accaggcggt gatcctggcg ctgagtccgt ttcacaatgc cgtgtatcag
    24421 gcattgtagg acggataagg cgttcacgcc gcatccggca ttcggtgcac gatgcctgat
    24481 gcgacgctgc cgcgtcttat caggcctaca aaatcaatcg cttttcagca acacctcttc
    24541 cagccactgg cgcagctcca cgggcgcggc tttcaggctg ttagatccac gcgtaatcgt
    24601 cgcgatgccc gcgccgagtt cattttttaa ctcacgctga ctcatttcgc cacgcaacag
    24661 ctcttcgaca atacgcacgc gagtccccaa cgcttcgcgc tcatctggcg tcagcatcag
    24721 gtttaacaac ggtaaatgga gatcgttttg gtaggcattc ttaagcaggt cgacaaaacg
    24781 taaccactcc tggtgacgct gttctgccat cgctgctgaa tagggtgatt gttgggccat
    24841 aatatgtcgc cattgttagc gggggaagca aaatgcctcc cccggttgta ctcgctaaag
    24901 agtacgatag catatcataa acgtgcggat cagtaacgac gtccccattc cgtggcgctc
    24961 atcaacgtcg gtttatcccc catgaaataa cggtagtaag cgtcataagc cagcacgttc
    25021 ttcacataac cgcgcgtctc ggagaacgga atactctcga caaatgccac agcgtcgata
    25081 cgcccggcgc tgttgccaag ccaggttcgc acccgtccag gtccggcgtt ataagctgct
    25141 gaggagaaaa tacggttatt gccaaactgc tgataaacat attgcaggta actggtgcca
    25201 atgttgatat tcgtttccgg atccagcaat tgcccagggc tgctataacc aggaatagag
    25261 aacatcttca ccgtatgggt cgctgtacca ggcataatct gcatcaggcc gctggcccct
    25321 accggtgatt tcactttcgg attccaggcg ctctcctgac gggcaatcgc catcgcatag
    25381 ctttgcggga tctctttccc actggtgtaa cgtttgaaaa gatcgttgta agccagcggg
    25441 aatcgctctt ccagatgatc ccaaagcttc ccggcgatcg ttgcctgaac gctaagatcc
    25501 caccattggt tattgaaagc ataccgagcc agttgagcct gctctgtttt tgacttgctc
    25561 ttcaccagat tggcccactc gctacgcgcg gtgttatcga gattccagta catcaactcg
    25621 cgcacgcgcg ccatctccgg cccctgagtc agggcgctgt caacattctg cggcgcttta
    25681 tcaatcttca gctcatactc ttctccgatg cgttgtgctg caaccatcgg gtagaaacca
    25741 cgctgttgca tgagttgatg caaaatctct ttcgcttcag cttcacgtcc gcgttccagc
    25801 aataaatcca cctgccagta acgccattca tctttctctt ttgcttccat cggcagacgc
    25861 gccagccagg tattcaggcc gcgacgatcg ccggtgccaa gtgccattcg cacacggcgt
    25921 tcaatcaacg acgtggattg cgagcgcata atgacgtcat cgcgccattt cgcctgctcg
    25981 tcggtgacat cgttgcccat caaacgccag gcgacgatat cgcgcagctc ctgaatctga
    26041 tcttcattaa gctgctgcac ctgggcaagc gacgggatca tcagccgtgc attttcagca
    26101 tcctgccgcg ccacactggc aaacgccacc gctgccattt gacgggtaaa atcggtcgct
    26161 ccggttgtac gcgcgaaggt cagtaccgta ttagggttgt tcgccagtga aatgattgcc
    26221 gaggcgatag tctggtaatc ggcaggcatc tgccctgcca gcactgttac caggccagtg
    26281 ttacccgctt tcatcgccag acggatacgc tctaaatacg ccagcggatc ttgtttacct
    26341 gacgcacgcc agacgctaaa taacttgtca caggcgttag gctggctctt gccggttagc
    26401 cacagctctt tcgccccttg ccaggcttct tcactctgcc cggtgttcca tttcgcatag
    26461 tagtaattac attgcgcttc ggtagttccg ggcttttccg ggctaaaggc taacaagcca
    26521 cgccagtctt cacgccgcgc cagttcattg acgaaacgag attgcagcgt gcgagcggga
    26581 ggaagcgtgg ggttagcgcg aacaaagtta gtgaccgtca ccgccggttg attcatcaga
    26641 tcatcggtga tctggcggta ttccaggtag ggataaagcg gataatcctt cagtccaggc
    26701 atcatttgtt cgaccacatc catttgtcga ttatcccagg cctgcttgat ttgcgcgtaa
    26761 cggctacgct gctcatccag tgagtcggct cgcgccacgc tgctgaccgt cagcagacag
    26821 acaccggcag ccaacagccg ccaggtaact tgtttggctt tttccacaag cgcatcctct
    26881 aagtgtaaat acatcaatgc agcgtcatgc cgcgtaatag atttatgcta accagtcatt
    26941 gccgtttacg ccacgttacg gacacttttt tacttttact gcgaaggtga tcgaccaact
    27001 gggattaggc ggggaaaaag gctacacttc gcctttgaaa actcatcatc acgataaaca
    27061 tagaggcgaa gtccaacgtg gctcaattcg tttataccat gcatcgtgtc ggcaaagttg
    27121 ttccgccgaa acgtcatatt ttgaaaaaca tctctctgag tttcttccct ggggcaaaaa
    27181 ttggtgtcct gggtctgaac ggcgcgggta agtctaccct gctgcgcatt atggcgggca
    27241 ttgataaaga catcgaaggt gaagcgcgtc cgcagccaga catcaagatt ggttacctgc
    27301 cgcaggaacc gcagctgaac ccggaacaca ccgtgcgtga gtccattgaa gaagcggttt
    27361 ctgaagtggt taacgccctg aaacgcctgg atgaagtgta tgcgctgtac gccgatccgg
    27421 atgccgattt tgacaagctg gccgctgaac aaggccgtct ggaagagatc attcaggctc
    27481 acgacggtca taacctgaac gtacagctgg agcgtgcggc ggatgcgcta cgtctgccgg
    27541 actgggacgc gaaaatcgct aacctctccg gtggtgagcg tcgtcgcgta gcgttgtgcc
    27601 gcctgctgct ggaaaaacca gacatgctgc tgctcgacga accgaccaac cacctggatg
    27661 ccgaatccgt ggcctggctg gaacgcttcc tgcacgactt cgagggcacc gtggtggcga
    27721 ttacccacga ccgttacttc ctcgataacg ttgcaggctg gatcctcgaa cttgaccgcg
    27781 gtgaaggtat tccgtgggaa ggcaactact cctcctggct ggagcagaaa gatcagcgcc
    27841 tggcgcagga agcttcacaa gaagcggcgc gtcgtaagtc gatcgagaaa gagctggagt
    27901 gggtacgtca gggaactaaa ggccgccagt cgaaaggtaa agcacgtctg gcacgctttg
    27961 aagagctgaa cagcaccgaa tatcagaaac gtaacgaaac caacgaactg tttattccac
    28021 ctggaccgcg tctgggcgat aaagtgctgg aagtcagcaa cctgcgtaaa tcctacggtg
    28081 atcgcctgct gattgatgac ctgagcttct cgatcccgaa aggggcaatc gtcgggatca
    28141 tcggtccgaa cggcgcgggt aaatcgaccc tgttccgtat gatctctggt caggaacagc
    28201 cggacagcgg caccatcact ttaggtgaaa cggtgaaact ggcgtcggtt gatcagttcc
    28261 gtgactcaat ggataacagc aaaaccgttt gggaagaagt ttccggcggg ctggatatta
    28321 tgaagatcgg caacaccgag atgccaagcc gcgcctacgt tggccgcttt aactttaaag
    28381 gggttgatca gggtaaacgc gttggtgaac tttccggtgg tgagcgcggt cgtctgcatc
    28441 tggcgaagct gctgcaggtt ggcggcaaca tgctgctgct cgacgaacca accaacgacc
    28501 tggatatcga aaccctgcgc gcgctggaaa acgccctgct ggagttcccg ggctgtgcga
    28561 tggttatctc gcacgaccgt tggttcctcg accgtatcgc cacgcacatc ctggactacc
    28621 aggatgaagg taaagttgag ttcttcgaag gtaactttac tgagtacgaa gagtacaaga
    28681 aacgcacgct gggcgcagac gcactggagc cgaagcgtat caagtacaag cgtattgcga
    28741 agtaatgcgt aaaatgccgg atgcggcgtg aacgccttat ccggcctaca aaccaagaaa
    28801 tttcaatacg ttgtgaatct ccaggcctga taagacgcgg taagcgtcgc atcaggcatt
    28861 gagcaccgaa tgccggatac ggcgtgaacg ccttttccgg cctacaacag ccttagtatc
    28921 cgcaatttcg tagcgggtca cccgtgcagg ttttttcaaa ccttgtttaa tctcgacagc
    28981 ctcttccaga gaggttttca actcatcaaa aaacttattt tatacctcgc ctttgagccg
    29041 tttcatcgcg gttatctctg ctcccccatc atctcccgca ccagctccac gcagcgcagg
    29101 aaacggctgt cataatcgtc ctcttcaacc cgcacgaatt cgatattgtt ctcttccagc
    29161 atctccacca gcaagttctg gaactctttg cgatccaccg aactgccgag gctgcgtaaa
    29221 ccatccgcca cccacggcgt gttgttctcc agcaggatca ccagatcgaa acggtattca
    29281 tcaatcaacg cctgtacgaa cggatgctca cgcccttcgt actttttgca gaacgcctgg
    29341 gtagtgacaa aatcagtgtc aataaacgcc actttattgg catatttcac tgcaaaatca
    29401 atgtattgtg cgtggcccag cgcgatttta tcgtaatcgg aatactgcaa tgcgatctca
    29461 tcaccgccga ggtgtgaaaa gacataatcg cgaccatatt cccacgcact ggtggtattg
    29521 aagatattgg caagtttgtt taccagggtg gatttaccgc tcgactcgcc accaaggatc
    29581 gccacggtac gtacaaagaa cggcttcact tccgtaggaa tatattccca gtagcggaac
    29641 gggttttcgc ggatctgcgc accgctgata ctcataaagg tacgtttcgg atcgaccagc
    29701 accgtatcga tccccagatg ttccatatac tgtggcgcat cggcttcttc cgaggtgtag
    29761 atcaggtccg gctgaatccc cttttcagcc ataaactttt tgatgccgtt gctccacaca
    29821 tcccagccgt gcggatacgg ctccatgccc tcttcgttga aagcatgaat gcgaatattt
    29881 ttctgatatt taaaagtttg taataaccag cgcagacgat ccggcaccgt gggctgctgc
    29941 gacatggcgc tgtcttcaaa caacgcgcgg tcgcgggtat cgtcaaaacc cataatgata
    30001 tgcagctcgt caacctggct acaggcgcgc tggataaggt agatatgtcc ggtatgcagc
    30061 gggtagaact taccgaatac gacaccaatg gttttcttct gtcggggaaa ttcaagcccc
    30121 aaaaaacggt gcaacgcctc cagcttttgc gcgctggggc ttttaatttt ggcattcagt
    30181 aactggctta aatacccttt ggtcataccg ctggcatcag ctacctgctg tagcgtgcag
    30241 ccctgttgct tgatggcagt tttcaggtaa tcaaatgacg acatatctcc ctccgtatct
    30301 ctcattataa gtcgtcgaac acgctaagcg cgtcggagag ttttttaacg ccaaaaatct
    30361 gcatcccttc cggcgctttt ttcggtacgt tagccgccgg aacaatcgcc cggcgaaaac
    30421 cgtgtttcgc cgcttctgaa attcgttcct gaccgctggg caccgggcgg atctcccctg
    30481 ccagcccgac ttcaccaaac accaccagat cctgtggcag cggtctgtca cgcaggctgg
    30541 aaaccatcgc cagcagtaac gctaaatcgg cactggtttc ggttaccttc acgccgccga
    30601 ccacgttcac aaatacatcc tgatcggcca tttgcagacc accgtgacgg tgcaagacag
    30661 ccagcaggat tgccagacgg ttttgttcca gccccactgc cacgcggcgc ggattcgcca
    30721 tcatcgagtg atcgaccagc gcctgaatct ccaccagcaa cggacgcgtt ccttcccaca
    30781 ccaccatcac ggagctgccg gaggtcactt catctccgcg acttaagaaa attgccgaag
    30841 ggttgctgac ttcacgcagc ccctgttcgg tcatcgcgaa gacgcccagc tcattcaccg
    30901 cgccgaagcg gtttttatgg ctgcgcaagg tgcgaaaacg ggagtcggca tcaccatcca
    30961 gaagcaccga acagtcgata cagtgttcca gcactttagg gccagccagc gagccatctt
    31021 tggttacgtg cccaaccatg acaatcgcca caccgcgcgt tttggcgaag cgcgtcagat
    31081 aagccgccgt ttcacgcacc tgcgccacgc taccaggcga tgattgtaca tccgccatat
    31141 gcatcacctg gatcgagtca attaccatca gtttcggttg ctcttcttcg gcaatcaggc
    31201 agatctgttc gatgctggtt tccgacaaca tattgagatt gtcagtcggc aggccaaggc
    31261 gatgagcgcg cattgccacc tgttgcagcg actcttcgcc agtgacatac agcgttttca
    31321 tctgctgggc cagtttgcac agcgtttgca gtagcagcgt ggatttcccc gcacctgggt
    31381 taccgccaat cagaatggcg cttcccggca ccacgccgcc gcctagtacg cggtcgaact
    31441 ctttaaatcc ggtggaaaaa cgcggcagct cttcaaggct gatatcggag agtttctgga
    31501 cttttgccac cccggcgcta ccggcatagc cgctgagacg ctcgttacgc gccaccgttg
    31561 gcgacgcagc aagacgcacc tcggtgatgg tgttccaggc atgacaggca ctgcactgcc
    31621 cctgccagcg cggataatcg gccccgcatt cattacaaac aaaggcgcgt tttggagctt
    31681 ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg atggcgggcg agcaattact
    31741 tctgattcag gctgccagag aggatgcaga atacccccat caggtcagcg tgacggatgg
    31801 tgacttccgc cttttcattc acttttggct tggcatggta ggcaatcccc agccctgctg
    31861 ctttgatcat cggcaggtca ttagctccat cgccaatcgc cacggtctgc gccagcggga
    31921 tttcatactc ctgcgcgagg cgagtcaaag ttttcgcttt gtactgcgcg tctacgatgt
    31981 cgccgatcac attgccggta aatttaccgt ccatgatctc cagttcattg gctaccacgg
    32041 cggtcaggcg cagcttgtcg cgcagatatt cagcaaagaa agtaaagccg ccggaggcaa
    32101 tcgccacttt ccagcccagc gtttccagct tgagcaccag ttgcgttaag cctggcatca
    32161 gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc agcgcctttc agcgtcgcca
    32221 cacggctgcg caggctggcg gtaaaatcga gttcgccgcg catcgcccgt tcggttactt
    32281 ccgccaccat ctcgcccgtt ccggccagtt tggcaatttc atcaatacat tcaatctgga
    32341 tggcggtgga atccatgtcc atcaccagca aacccggcgt gcgcaggtgc gggattttcc
    32401 ccagcggggc gacatccagt tgcgcttcat gggccaggcg cgtagcccgt gcggtgagtg
    32461 aacctgccag acgaatcacc tgataatctt ccacgcacca ggcggcaaca atcaccatcg
    32521 ccgcacccag tttgctctgg tattgggtca gacgctgttt atccagccca cgaccataca
    32581 gcagccagcc gctacgacct gcgtggtaat ccagtggcat cacttcatca ccacttaatg
    32641 aaagaggcag acccggccat aaagagacat cttcaggcag gtcgcaccag gtaatgttag
    32701 gcattaaggc tcctgtaaaa tcgttcgaag cagggaaaat aacgcatgag gctaccttgt
    32761 atccattgct tctggcaaca ttaagtctca aattttcaaa gggtggaaga tggctcgcac
    32821 aaaactgaaa ttccggctgc atcgggcagt gattgtcctg ttctgtcttg ccttgttagt
    32881 ggcactgatg cagggggcgt catggtttag tcaaaaccac cagcgacagc gtaatccaca
    32941 gctggaagaa ctggcccgca ccctggcgcg tcaggtgacg ctgaacgttg caccgctgat
    33001 gcgtaccgac tcaccagatg aaaaacgcat tcaggcgatc ctcgatcagt taacggatga
    33061 aagccgcatc ctcgacgcgg gtgtgtatga cgaacaaggc gatcttatcg cccgttcagg
    33121 cgaaagcgtc gaagtgcgcg accggctggc gctcgacggt aaaaaagcag gcggctattt
    33181 taaccagcag attgtcgagc caattgcggg taaaaacgga ccgctcggct atctgcgcct
    33241 gacactcgac acccatacgc tcgccaccga agcccaacag gtggataaca ccactaacat
    33301 tttacgcctg atgttgctgc tctcactggc aatcggcgta gtgctgaccc gcacgctgct
    33361 acagggtaaa cgcacccgct ggcagcaatc gcccttcctg ttaaccgcca gcaaaccggt
    33421 gccggaagag gaagaaagcg agaaaaaaga gtgacccatt actacaagaa aggaaatcgt
    33481 tatgtccaca ttacgcctgc tcatctctga ctcttacgac ccgtggttta acctggcggt
    33541 ggaagagtgt atttttcgcc aaatgcccgc cacgcagcgc gttctgtttc tctggcgcaa
    33601 tgccgacacg gtagtgattg gtcgcgcgca gaacccgtgg aaagagtgta atacccggcg
    33661 gatggaagaa gataacgtcc gcctggcgcg gcgcagtagc ggtggcggcg cggtgttcca
    33721 cgatctcggc aatacctgct ttacctttat ggctggcaag ccggagtacg ataaaaccat
    33781 ctctacgtcg attgtgctca atgcgctgaa cgcgctcggc gtcagcgccg aagcgtccgg
    33841 acgtaacgat ctggtggtga aaacagtcga aggcgaccgc aaagtctcag gctcggccta
    33901 tcgcgaaacc aaagatcgtg gcttccacca cggcactttg ctgctcaatg ccgaccttag
    33961 ccgcctggca aactatctca atccggataa aaagaaactg gcggcgaaag gcattacctc
    34021 agtgcgttcc cgcgtgacca acctcaccga gctgctgccg gggatcaccc atgagcaggt
    34081 ttgcgaggcc ataaccaagg cctttttcgc ccattatggc gagcgtgtag aagcggaaat
    34141 catctccccg gacaaaacgc cagacttgcc aaacttcgcc gaaacctttg cccgtcagag
    34201 tagctgggaa tggaacttcg gtcaggctcc ggcattctcg catctgctgg atgaacgctt
    34261 tagctggggc ggcgtggaac tgcatttcga cgttgaaaaa ggccatatca cccgcgccca
    34321 ggtgtttacc gacagcctca accccgcgcc gctggaagcc ctcgccgggc gactgcaagg
    34381 ctgcctgtac cgcgcggata tgctgcaaca agagtgcgaa gcgctgttgg ttgacttccc
    34441 ggaccaggaa aaagagctac gggagttgtc gacgtggata gcgggggcgg taaggtaact
    34501 cgctgattaa aataccaaag gaagggaata tgatcgatct ggaaaaccag gagcgggaga
    34561 ttatcaattt aatgttaagc cagcgtattt cctggctagc cgccgtgcgt atcaggcata
    34621 aattatcgct tgctgaagta agtaaaatgt taggtatctc gattaattct ctaaaataga
    34681 tcgaaaagac ggaacggctt agctcaaaca tcaaaagcaa aatgggagaa atttatggtt
    34741 gtccaccaga gttgctgata tgcccttcct ggatgacggc tgaacataaa tgaatagatt
    34801 cgtgaataga tacttgaatg tattttattt tttatccata tgaaaaataa tagtaaatct
    34861 ataaatcagt tgctattgat gaatagatac taaaaagccg gagcagtccc ccactccggc
    34921 ttaacacgac aatcgccttt cagcgaaaca caattactct ttatcgccca gcagaacgga
    34981 ttccagtgcg attttgatca tgtcgttgaa ggtggtctga cgctcagcgg cagtggtctg
    35041 ctcgtgagtg cggatgtggt cagacacagt gcagatggtc agggctttcg cgccaaattc
    35101 tgcagcgacg ccgtagatac cagccgcttc catttccacg ccgaggatgc cgtatttttc
    35161 catcacgtcg aacatttcgc cgtccggaga gtagaacagg tcagcggaga acaggttacc
    35221 aacgcgagca tcaatgccca gtgctttagc cgcgtctacc gcgttacgca ccatgtcaaa
    35281 gtcagcgata gcggcaaagt catgatcttt aaaacggatg cggttaactt tggaatcggt
    35341 gcaggcaccc ataccgataa cgacgtcgcg cagttttacg tgcggcagaa ctgcgccaca
    35401 ggaacccacg cggataattt tcttcacgcc gaaatcggtg atcagttctt tggtgtagat
    35461 ggagcaggac gggataccca taccgtgacc cattacggaa attttgcggc ctttgtaagt
    35521 accggtgaag cccagcatac cgcgaacgtt gttcacttca cgggcatctt caaggaaagt
    35581 ttcagcaata tacttcgcac gcagcgggtc gcctggcatc aaaactacgt cagcgaaatc
    35641 gcccatttct gcattaatgt gtggggtagc cattgtttta tccttatcaa catgttttca
    35701 ccctctccct gagggagagg gccggggtga gggcatcaag ccgcacccat tatcagaaca
    35761 tggctttgcc atattccata tcagaagtac caaaatattt tgccagagtc tggccgatat
    35821 ccgcgaaggt ttcacggtga cccagtgagc ccggttttac tttcgggccg taaaccagta
    35881 ccggaatgtg ttcacgcgtg tggtcagtac cggtccaggt cggatcacaa ccgtggtcag
    35941 cagtgaggat caggatgtcg tcatcgcgca gcagagacat cagctccggc agacggcggt
    36001 cgaacagctc cagacccaca gcataaccgg cgacgtcgcg acggtggccc caggaagagt
    36061 cgaagtcaac gaagttggtg aagacgatgg tgttatcacc cgcttctttc atctctttga
    36121 tggtggcgtc aaacagcgcg tccaggccag ttgctttcac tttcttggtg ataccgcagt
    36181 tggcgtagat gtctgcaatt ttaccgacag aaaccacctg accgtgtttt tcatcaacca
    36241 gtttctgcag cacggtcggt gctggcggct caacagccag gtcgtgacgg ttaccggtac
    36301 gctggaagtt accggctttg tcaccgataa acggacgagc gataacacga ccgatgttgt
    36361 agccgccgtt ggtcagctct tcacgggcga tttcgcacag ttcgtagagt ttatccaggc
    36421 caaaggtttc ttcgtggcag gcaatctgga acacagagtc agcggaggta tagaaaatcg
    36481 gcttgccggt tttcatgtgc tcttcgccca gctgatccag aatgaccgta ccggaagagt
    36541 ggcagttacc gaggtaaccc ggcagattag cgcgttcaac cagtttatcc agcagctctt
    36601 gcgggaagct gttttcgtga tcggagaaat atccccactc aaatagaacc gggacaccgg
    36661 cgatttccca gtgaccagac ggggtatctt taccggatga catttcgtgc gcccatgcgt
    36721 acgcgccgat aacttcagcg ttgccgtcca ttcccgccgg aatgaaaccg gtagaacctt
    36781 cgtgtgcttt cgccagcccc agacgggtca gatttggcag attgagcgga cctttacgac
    36841 cgttatcagc ttcgcctttg gcacaagctt ctgcgatatg acccagggtg tcagccccga
    36901 cgtcaccaaa gcgttctgca tcttctgtag cgccgatgcc gaatgagtcc agcaccataa
    36961 taaatgcacg tttcatatgt tctccgtact cagtgcttca aatttaaatg atcagatcag
    37021 tataccgtta ttcgctgata cggcgataga cagttggtgt gctttccggt gctttatcgg
    37081 caagtttaat tgccgctttc accgctttcg ccgcttcctg ccagctgttt tcgtctttcg
    37141 cgtggataac cgccagcgga cgctgaccgt ctacctggtc gcccaaacgc gccatatcag
    37201 taaagccgac gctgtaatca atggtgtcag atgcctgacg acgaccgccg cccattgcaa
    37261 ccactgccat ccccagcgcg cgggtatcca tttcactgac aaaaccttcg gtatcagcat
    37321 agactgcttt cgtcagcatc gctgtcggca gatacttcgc gtagttctca acgaagtcgg
    37381 ttgggccttt ttgtgccgct accatacgac caaagacttc tgctgcttta ccgttgtcca
    37441 gcactgcttg caatttcgcg cgcgcttcgg cgtcatcttt cgccagtttg ccggagataa
    37501 gcatctccac gcacagcgcc atcgtgacat caaacagacg cgggttacga tactcacccg
    37561 tcaggaactg caccgcttca cgaacttcaa ccgcgttacc tgcactggag gccagtacct
    37621 gattcatgtc ggtgagcagc gcggtggtgc gcacgccagc gccgttagcc acgccaacaa
    37681 tcgcttcggc aagggcttca gagagttcgt aggtcggcat aaacgcgccg ctacccactt
    37741 tcacgtccat caccagcgcg tccagacctt ccgcaagttt cttcgccaga atagaggcgg
    37801 tgatcagcgg gatggagtcc acggttgcgg taatatcacg ggtcgcgtag aaacgtttat
    37861 ccgccggagc cagtgagctg gtctgaccga taatcgccac gccgacgtct ttaataattt
    37921 cgcggaaacg gttgtcatcc gggaaaatgt cgaagccagg gatggattcc agtttgtcga
    37981 gcgtaccgcc agtatgaccg aggccgcgac cagagatcat cggaatatag ccgccgcagg
    38041 ctgcgaccat cggccccaac atcagcgaag tcacatcgcc gacgccacca gtggaatgtt
    38101 tatcaacaat cgggccattc agatgcaggc ttttccagtc gagaacggtt cctgaatctc
    38161 gcatcgccat ggtcagcgag acacgctcag gcattgtcat atcgtggaag aaaatggtca
    38221 tcgcgagggc ggcaatctgc ccttcggaga tagtgttgtc gcgaatgccg ttgataaaga
    38281 aacgaatttc ttcatcgctc agtgcatgac catcacgttt tttacgaata atttcttgtg
    38341 cgagaaacaa ggtaacctcc cgaggaaaag agtatgaggc ggcttttgcc ggatgcgtct
    38401 acgccttatc cggcctacgg gtagatacgt ctcgtaggcc tgataagcgc agcgcatcag
    38461 gcgtaaagca tcttacttag tagctgctgg cgctcttacc gtcaccgtgg cccagcgctt
    38521 tcaacaggct tgccagcagg ctggaagcac caaagcggta gtgacgcgca tctgcccagt
    38581 cagcaccgaa cagttcatct gcgatggcga gatatttctg cgcatcttcc gcagtacgca
    38641 cgccgcccgc tggtttgaaa ccaacggttt tttctacgcc catatcacgg atcacttcca
    38701 tcatgatgcg cgcgctttcc ggcgtcgcgt tcacagccac tttaccggta gaggttttga
    38761 tgaagtccgc acccgctttg atggagattt cagacgcttt acggatcagc gcttcgtctt
    38821 tcagttcgcc ggtttcgatg atcactttca gcagtacatt cgctgccgcg caagcctctt
    38881 tacaggcttt caccaggtca aaaccaacct gctcgttacc cgccatcagc gcgcggtacg
    38941 ggaacacaac gtcaacttca tcagcaccgt aggcgattgc cgcacgggtt tctgccagcg
    39001 cgatttcgat gtcatcgtta ccgtgtggga agttggttac cgtagcgatt cggatttccg
    39061 gggtgccctg ctctttcagc gttttgcgag caatcgggat aaagcgagga tagatacaga
    39121 tagcggcggt attgccgacc ggggttttgg cctgatgaca cagagcaatt actttctcgt
    39181 cggtgtcgtc gtcattcagg gtggtcaggt ccatcaattt cagtgcacgc aggctgcttg
    39241 ctttcagatc agtcatttca ttctcctggc ttgtcgccaa taaaattcac cttgcgagtt
    39301 tgttagtatt ctaacatcta ctccgcaaca cacttcgata tacatcacaa ttaaggaaat
    39361 ctacttacaa gtttgcatca ctgtaatgcg atctggttca aataattcac tttcaaatga
    39421 atgcgtcagt ggtggcaaac gcatcaggat caaagtgaac atcacgaaac ttcttacaat
    39481 ggcgcatcac cttcggcata aaaggaaaag aagatgccca cttctcatga aaatgcactg
    39541 caacaacgtt gccagcaaat tgtcaccagc ccagtgctta gcccggagca gaagcgccat
    39601 tttctggcac tggaagcaga aaacaatctg ccttatccgc agcttcctgc cgaagcccgc
    39661 cgcgcgctgg atgagggtgt aatctgcgat atgtttgaag gtcatgcgcc gtacaaaccg
    39721 cgctatgtct tacccgatta cgcccgtttt ctggcgaacg gttccgaatg gctggagctg
    39781 gaaggcgcga aagatcttga tgacgcactc tctctgctga ccattcttta ccaccacgta
    39841 ccgtcggtca catcgatgcc ggtctacctg gggcaactgg atgcgttgtt gcaaccatat
    39901 gttagaattc taacacaaga cgagatcgat gttcgaataa aacgtttctg gcgttacctc
    39961 gacagaaccc tgccagacgc ctttatgcac gccaatatcg gcccgtctga ttcgcccatt
    40021 acccgcgcga tcttacgtgc agacgcagag ttgaagcagg tttcaccgaa cctgaccttt
    40081 atctacgatc ctgaaatcac ccctgatgac ctgctgctgg aagtggcgaa gaacatctgt
    40141 gaatgtagca aaccgcacat cgccaacggt ccggtgcatg ataaaatttt cacaaaaggg
    40201 ggctacggga ttgtgagctg ttacaactca ctgccgctgg cgggtggtgg cagcacgctg
    40261 gtacgcctta acctgaaagc cattgccgag cgcagcgaat cgctggatga cttctttacg
    40321 cgcactctac cgcactactg ccagcagcag atcgccatca tcgatgcgcg gtgtgaattc
    40381 ctctatcaac aatcacactt ctttgagaat agcttcctgg tgaaagaagg gctgattaac
    40441 cctgaacgtt ttgtgccaat gtttggcatg tatgggctgg cggaagcggt taacttgctg
    40501 tgtaagaaag aagggattgc cgcgcgctac ggtaaagaag ccgccgcaaa tgaagtgggt
    40561 tatcgcatca gcgcgcaact ggcggagttt gtcgccaata cccccgtgaa atatggctgg
    40621 caaaaacgcg ccatgttaca cgcacagtcg gggatcagtt ccgatatcgg caccacgccg
    40681 ggcgcgcgtt tgccgtatgg tgatgagcca gatccgatca cccatctgca aactgtcgcc
    40741 ccgcatcatg cttattatta ttccggcatc agcgacattc tgacgctcga cgaaaccatc
    40801 aaacgtaatc cgcaggcgct ggtacagctt tgcctcggtg cctttaaagc cggaatgcgt
    40861 gaatttactg ccaatgtcag cggtaacgat ctggttcgcg ttaccggtta tatggtgcgt
    40921 ttgtcggatt tagaaaaata tcgcgccgaa ggttcacgca ccaacaccac ctggctgggc
    40981 gaagaagccg cacgcaacac tcgtattctg gaacgccagc cgcgcgtgat aagccatgaa
    41041 cagcagatgc gctttagtca gtaagattat ccccttctcc tgcgttgacg ggccaggcag
    41101 tcgtctggct ctgtttttgc agggctgcaa tctgcgctgc aaaaactgtc acaatccgtg
    41161 gacgatggga cgttgcaatg actgtgggga gtgcgtgcca cagtgtccgc atcaggcgtt
    41221 gcagattgtt gacggcaaag tggtgtggaa cgctgtggtt tgcgagcagt gtgatacctg
    41281 cctgaagagg tgtccgcaac atgccacgcc aatggcgcaa tccatgagcg tggacgaagt
    41341 gcttagccat gtccgcaaag cggtgctgtt tattgaaggg ataacggtaa gcggcggcga
    41401 agccacgacc caactgccgt ttgtagtggc gctgtttact gctatcaaaa acgatccgca
    41461 actgcgccac ctcacctgcc tggtggacag taacggcatg ttgagcgaaa ccggctggga
    41521 aaaattactc ccggtgtgtg acggcgcaat gctcgatctc aaagcgtggg ggagcgaatg
    41581 tcatcaacac ttaaccggac gcgataatca gcagattaag cgcagcatct gtttgcttgc
    41641 agagcgcggc aagctggcgg aactgcgttt gctggtaatt ccagaccagg tggattattt
    41701 gcatcacatc gatgagctgg cgacgtttat caagagactt ggcgatgttc cggttcgcct
    41761 gaatgcgttt catgcccacg gcgtgtatgg cgaggcgcaa agctgggcga gcgccacgcc
    41821 ggaagacgtt gagccgttgg ctgatgcgtt aaaggtgcgc ggggtgagcc ggttgatatt
    41881 tccggcgctc tatttgtgac atgccggata cggcctacgg ttcggcacag acttgtaggc
    41941 atgataagac gcgacaagcg tcgcatcagg cattatgcgc cgactgccgg atgcggcgtg
    42001 aacgccttat ccggcctacg gttcggcaca gacttgtagg catgataaga cgcgtcagcg
    42061 tcgcatcagg cattgtgcgc caactgccgg atgcggcgtg aacgccttat ccggcctacg
    42121 gttcggcaca gac
//
LOCUS       DAESHI010000025        41689 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.025, whole genome shotgun sequence.
ACCESSION   DAESHI010000025 DAESHI010000000
VERSION     DAESHI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41689
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.025"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..1467
                     /locus_tag="KW011_001393"
     CDS             <1..1467
                     /locus_tag="KW011_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HBH7659773.1"
                     /translation="MASTYPANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLV
                     AFPQLMALMGENPQWVQNLGDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITT
                     TKKAVPVKQTVTAPVIPSNTVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYG
                     NWANTAYPPVYLPPPAGEPFVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDD
                     DDYHHHDGGHRDGNGWQHNGDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYH
                     DQDMAKRFHQTDVNGGMSATQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAA
                     QRFNEAEHYGSYDDFRDFSRRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQ
                     QRREAARERIQSATPEQRQVFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQ
                     ESRPQRLNDSNHTVRLNNEQRSAVRERLSERGARRQER"
     gene            complement(1538..2962)
                     /gene="lpdA"
                     /locus_tag="KW011_001394"
     CDS             complement(1538..2962)
                     /gene="lpdA"
                     /locus_tag="KW011_001394"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBH7659774.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(3287..5179)
                     /gene="aceF"
                     /locus_tag="KW011_001395"
     CDS             complement(3287..5179)
                     /gene="aceF"
                     /locus_tag="KW011_001395"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBH7659775.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(5194..7857)
                     /gene="aceE"
                     /locus_tag="KW011_001396"
     CDS             complement(5194..7857)
                     /gene="aceE"
                     /locus_tag="KW011_001396"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HBH7659776.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(8018..8782)
                     /gene="pdhR"
                     /locus_tag="KW011_001397"
     CDS             complement(8018..8782)
                     /gene="pdhR"
                     /locus_tag="KW011_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HBH7659777.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            9323..10696
                     /gene="aroP"
                     /locus_tag="KW011_001398"
     CDS             9323..10696
                     /gene="aroP"
                     /locus_tag="KW011_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HBH7659778.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(10739..11593)
                     /gene="ampE"
                     /locus_tag="KW011_001399"
     CDS             complement(10739..11593)
                     /gene="ampE"
                     /locus_tag="KW011_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HBH7659779.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(11590..12141)
                     /gene="ampD"
                     /locus_tag="KW011_001400"
     CDS             complement(11590..12141)
                     /gene="ampD"
                     /locus_tag="KW011_001400"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HBH7659780.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            12229..13122
                     /gene="nadC"
                     /locus_tag="KW011_001401"
     CDS             12229..13122
                     /gene="nadC"
                     /locus_tag="KW011_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBH7659781.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            13325..13765
                     /gene="ppdD"
                     /locus_tag="KW011_001402"
     CDS             13325..13765
                     /gene="ppdD"
                     /locus_tag="KW011_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HBH7659782.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            13775..15160
                     /gene="gspE"
                     /locus_tag="KW011_001403"
     CDS             13775..15160
                     /gene="gspE"
                     /locus_tag="KW011_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HBH7659783.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVNQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            15150..16352
                     /gene="hofC"
                     /locus_tag="KW011_001404"
     CDS             15150..16352
                     /gene="hofC"
                     /locus_tag="KW011_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HBH7659784.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPTLLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(16387..17430)
                     /gene="guaC"
                     /locus_tag="KW011_001405"
     CDS             complement(16387..17430)
                     /gene="guaC"
                     /locus_tag="KW011_001405"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBH7659785.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(17586..17630)
                     /locus_tag="KW011_001406"
     CDS             complement(17586..17630)
                     /locus_tag="KW011_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659786.1"
                     /translation="MIIKTLKMSARKRN"
     gene            17655..18275
                     /gene="coaE"
                     /locus_tag="KW011_001407"
     CDS             17655..18275
                     /gene="coaE"
                     /locus_tag="KW011_001407"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBH7659787.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            18275..19018
                     /gene="zapD"
                     /locus_tag="KW011_001408"
     CDS             18275..19018
                     /gene="zapD"
                     /locus_tag="KW011_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HBH7659788.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            19028..19225
                     /gene="yacG"
                     /locus_tag="KW011_001409"
     CDS             19028..19225
                     /gene="yacG"
                     /locus_tag="KW011_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HBH7659789.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(19325..19714)
                     /gene="mutT"
                     /locus_tag="KW011_001410"
     CDS             complement(19325..19714)
                     /gene="mutT"
                     /locus_tag="KW011_001410"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HBH7659790.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(19774..22479)
                     /gene="secA"
                     /locus_tag="KW011_001411"
     CDS             complement(19774..22479)
                     /gene="secA"
                     /locus_tag="KW011_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBH7659791.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(22541..23053)
                     /gene="secM"
                     /locus_tag="KW011_001412"
     CDS             complement(22541..23053)
                     /gene="secM"
                     /locus_tag="KW011_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HBH7659792.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(23284..24201)
                     /gene="lpxC"
                     /locus_tag="KW011_001413"
     CDS             complement(23284..24201)
                     /gene="lpxC"
                     /locus_tag="KW011_001413"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HBH7659793.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(24302..25453)
                     /gene="ftsZ"
                     /locus_tag="KW011_001414"
     CDS             complement(24302..25453)
                     /gene="ftsZ"
                     /locus_tag="KW011_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBH7659794.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(25514..26776)
                     /gene="ftsA"
                     /locus_tag="KW011_001415"
     CDS             complement(25514..26776)
                     /gene="ftsA"
                     /locus_tag="KW011_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBH7659795.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(26773..27603)
                     /gene="ftsQ"
                     /locus_tag="KW011_001416"
     CDS             complement(26773..27603)
                     /gene="ftsQ"
                     /locus_tag="KW011_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HBH7659796.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(27605..28525)
                     /gene="ddlB"
                     /locus_tag="KW011_001417"
     CDS             complement(27605..28525)
                     /gene="ddlB"
                     /locus_tag="KW011_001417"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBH7659797.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(28518..29993)
                     /gene="murC"
                     /locus_tag="KW011_001418"
     CDS             complement(28518..29993)
                     /gene="murC"
                     /locus_tag="KW011_001418"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBH7659798.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(30047..31114)
                     /gene="murG"
                     /locus_tag="KW011_001419"
     CDS             complement(30047..31114)
                     /gene="murG"
                     /locus_tag="KW011_001419"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBH7659799.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(31111..32355)
                     /gene="ftsW"
                     /locus_tag="KW011_001420"
     CDS             complement(31111..32355)
                     /gene="ftsW"
                     /locus_tag="KW011_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HBH7659800.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(32355..33671)
                     /gene="murD"
                     /locus_tag="KW011_001421"
     CDS             complement(32355..33671)
                     /gene="murD"
                     /locus_tag="KW011_001421"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBH7659801.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(33674..34756)
                     /gene="mraY"
                     /locus_tag="KW011_001422"
     CDS             complement(33674..34756)
                     /gene="mraY"
                     /locus_tag="KW011_001422"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBH7659802.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(34750..36108)
                     /gene="murF"
                     /locus_tag="KW011_001423"
     CDS             complement(34750..36108)
                     /gene="murF"
                     /locus_tag="KW011_001423"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBH7659803.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLNAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(36105..37598)
                     /gene="murE"
                     /locus_tag="KW011_001424"
     CDS             complement(36105..37598)
                     /gene="murE"
                     /locus_tag="KW011_001424"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBH7659804.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(37585..39351)
                     /gene="ftsI"
                     /locus_tag="KW011_001425"
     CDS             complement(37585..39351)
                     /gene="ftsI"
                     /locus_tag="KW011_001425"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HBH7659805.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(39367..39732)
                     /gene="ftsL"
                     /locus_tag="KW011_001426"
     CDS             complement(39367..39732)
                     /gene="ftsL"
                     /locus_tag="KW011_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBH7659806.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(39729..40670)
                     /gene="rsmH"
                     /locus_tag="KW011_001427"
     CDS             complement(39729..40670)
                     /gene="rsmH"
                     /locus_tag="KW011_001427"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBH7659807.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(40672..41130)
                     /gene="mraZ"
                     /locus_tag="KW011_001428"
     CDS             complement(40672..41130)
                     /gene="mraZ"
                     /locus_tag="KW011_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBH7659808.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT         9837 a        11656 c        10299 g         9897 t
ORIGIN      
        1 atggcatcaa cctatccggc aaacgttgct caagcagtgc aatggtcgca cgataatcca
       61 cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga cgccagcgtt
      121 aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc gcaatgggtg
      181 caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc ggtacagcga
      241 ttgcggcaac tggcgcaaca aaccggctcg ctgaagtcat caaccgaaca gaaagttatt
      301 accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt cataccatcc
      361 aataccgttt taactgccaa ccccgtcatt acagagccgg caacaaccgt catttccatt
      421 gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt ggtttacggg
      481 aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg agaaccgttt
      541 gttgacagct ttgtgcgcgg attcggctat agcatgggcg ttgctaccac gtacgcacta
      601 tttagcagca tcgactggga tgacgacgat catgaccatc atcatcatga cgatgatgat
      661 tatcatcacc acgatggcgg tcatcgtgac ggtaatggct ggcaacacaa cggcgacaac
      721 atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac tgataagaat
      781 atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga tcaggatatg
      841 gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga gtgccacgca gctacctgct
      901 ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg aacacacgcc
      961 gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt taatgaagct
     1021 gaacactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc actgacccag
     1081 caacaaaagg acgccgctcg tcagcgttat caatcggcct ctcctgagca gcgccaggcg
     1141 gttcgcgaga aaatgcagac taacccacag aaccagcagc gaagagaggc agcgcgtgag
     1201 cgtattcagt ctgcaactcc cgaacagcgc caggtgttta aggaaaaagt acagcagcgc
     1261 ccactgaacc aacagcaacg tgataacgcc cgccagcgta tacaatcggc atcacctgaa
     1321 caacgtcagg tttttcggga aaaagttcag gagagccgcc cacaacgtct aaacgacagt
     1381 aaccatactg tcaggctgaa taacgagcaa cggtcagcag tacgcgaacg tctctctgag
     1441 cgcggagcaa ggcgacagga aaggtaaatt gcagacgtaa aaaaagcggc gtggttagcc
     1501 gcttttttaa ttgccggatg ttccggcaaa cgaaaaatta cttcttcttc gctttcgggt
     1561 tcggcaggtc ggtaatgcta ccttcgaaca cttctgccgc caggcccaca gactcgtgca
     1621 gagtcgggtg cgcgtggatg gtcagtgcga tgtcttcagc atcacaaccc atttcgattg
     1681 ccagaccgat ttcacccagc agctcaccgc cgttagtacc gacaatcgca ccaccgatca
     1741 cacggtgaga ttctttgtcg aaaatcagct tggtcatacc gtctgcgcag tcggaagcga
     1801 tagcacgacc agaagcagcc cacgggaagg tggcggtttc atagctgatg cctttctctt
     1861 tcgcttcttt ctcagtcaga cctacccatg caacttctgg ttcggtatag gcgatagacg
     1921 ggataacttt cggatcgaag tagtgttttt taccggcgat aacttcagcg gcaacgtgac
     1981 cttcgtgaac acctttgtgt gccagcatcg gctgaccaac gatatcgccg atagcaaaga
     2041 tgtgcggtac gttggtacgc agctgtttgt caacgcggat gaaaccacgg tcgtccactt
     2101 ccacgccagc tttgcctgcg tcgaggtttt taccgttcgg cacacgacca atcgctacca
     2161 gcacggcgtc gtaacgctgc ggttcagcgg gtgctttttt gccttccatc gtcacataaa
     2221 taccgtcttc tttcgcttca acggcggtaa ctttggtttc cagcatcagg ttgaatttct
     2281 tgctgatacg cttggtgaag actttaacga tgtctttgtc agctgccggg ataacctggt
     2341 cgaacatttc aaccacgtca atctgtgaac ccagcgcgtg gtatacggtg cccatttcca
     2401 gaccgatgat accgccaccc attaccagca ggcgttctgg tacttctttc agttccagcg
     2461 cgtcagtgga gtcccagata cgcggatctt catgcggaat aaacggcagt tggatcgggc
     2521 gagaacccgc tgcaatgatc gcattgtcga agttgatcac ggttttgccg ttctcacctt
     2581 caacttccag ggtgttagcc ccggtgaatt tacccagacc gttgaccact ttgactttgc
     2641 ggcctttcgc cataccagcc agaccaccgg tcagctgatt gatcactttc tctttccaag
     2701 tacgaatctt gtcaatatcg gttttcggtt cgccgaagac gataccgtgt tcagccagcg
     2761 ctttggcttc ttcgataact tttgctacgt gcagcagtgc tttagaaggg atacagccga
     2821 cgttcaggca aacaccgcca agggtgttgt aacgttctac gattacggtt tccagaccta
     2881 aatcagcgca acggaaggca gcggagtaac ctgcggggcc tgccccaagt accacgacct
     2941 gagttttgat ttcagtactc atcatgacct ctatatattt atctccggcg gtcatacccg
     3001 tcgtctttca ggccgcagcg gcgttagctt cacgccctca ccccagtcac ttacttatgt
     3061 aagctcctgg ggattctggc gcttgctacc ttgctgcacc cagaaatcca tagggtatgt
     3121 gaagtaatac cctaaccacc accgggtcgt tctatccgtc ggtattttac aaaattgtta
     3181 acaattttta aacaacaaac ggcaaccgat ttgtctattc gctaataacc tcaatacatt
     3241 catgagatta ccagaaaaaa gccggccgtt gggccggctc ttttacttac atcaccagac
     3301 ggcgaatgtc agacagcgtg ttgttaatga tggtaatgaa acgggcacca tcagcaccgt
     3361 cgatcacgcg gtggtcgaag gagagagaaa tcggcagcat cagacgcggc acgaactctt
     3421 taccattcca caccggctcc atcgcggact tggaaacgcc gaggatagcc acttccggcg
     3481 cgttcacaat cggcgcgaag tgggtagtac ccaggccgcc gatgctggag atggtgaagc
     3541 aaccgccctg catttcgccc gcagtcagct taccgtcacg cgctttctta gaaatagtca
     3601 tcagctcgcg agacagctcg atgatgcctt tcttgttgac gtctttgaat accggaacaa
     3661 ccagaccgtt cggggtatcc accgccacac cgatgttgat gtatttcttc agggtcagac
     3721 gctgaccgtc ttccgacagc gaactattga agcgaggcat ctgctcaaga gctgcagcaa
     3781 cggctttcat gatgaagaca accggggtga gcttcacatc cagcttacgt ttcgccgctt
     3841 cttcgttctg ctgtttacgg aacgcttcca gctcggtgat atcggttttg tcgaagtggg
     3901 taacatgcgg gatcatcacc cagttacggc tcaggttagc accagaaatt ttctggatac
     3961 ggcccagttc cacttcttcg atttcgccaa acttgctgaa gtccaccttc ggccacggca
     4021 gcatgccagg gataccaccg ccagtcgccg ccggagctgc ttctgcacgt ttgatagctt
     4081 ctttcacgta agcctgaacg tcttcgcgca ggatacgacc tttacgacca gtgcccttca
     4141 ctttcgccag gttaacgcca aactcgcgtg ccagacggcg gatcagcgga gtcgcgtgaa
     4201 cgtaagcgtc gttttcagca aattcagatt tgccttcggc tttcgcagcc ggagctgctg
     4261 ccggggcttc agctttcgct gccggagccg gcgctgccgc ttcctgtttc gcaggagctg
     4321 ccgcaggcgc tgcgccttca acttcgaaga tcataatcag cgagccagtt ttcactttat
     4381 cgccaacgtt gactttcagt tccttcacga cgcctgcaaa cggagccggg acctccatag
     4441 aagctttatc gccttctacg gtgataagcg attgttcagc ggcaactttg tcgcccactt
     4501 tcaccatcac ttcggtcact tcaacttcgt caccgccgat atccggaacg ttaacttctt
     4561 tcacgccagc cgctgatgca ggggccgctg ccggagctgc ttcctgttta gccgccggag
     4621 ctgccgcgcc tgcttcaccc gcgacttcga agaccataat cagcgagccg gtagacactt
     4681 tgtcacccac gttcactttg atctctttca cggtgccagc aaacggagcc ggaacttcca
     4741 tagaagcttt atcgccttct acggtgatca gcgactgttc agcttcaact ttatcgccaa
     4801 ctttcaccag gatttcggtc acttcaactt cgtcgctgcc gatatccgga acgttaacgt
     4861 cttttgccgc cgcagccgcg ggtgctgctg ccggagctgc ttctttcttc tcttctgcct
     4921 gagcaggtgc agcgtctgct gcaccgtcgg cggaatcgaa aatcataatc agtgcgccgg
     4981 tctgggtttt atcgccaaca gagactttga tctctttaac gatacccgcc tgcggagacg
     5041 gaacttccat agaggctttg tcgccttcta cggtgatcag cgactgttcg gcttcaactt
     5101 tgtcgcccac tttgaccagg atctcggtga tttcaacttc atcagccccg atgtccggta
     5161 ctttgatttc gatagccatt attcttttac ctcttacgcc agacgcgggt taactttatc
     5221 tgcatcgatg ttgaatttgg cgattgcgtc agcaaccact ttcttatcga tttcgccacg
     5281 tttagccagt tcgcccagcg ccgcaaccac gacataagaa gcatcaactt cgaagtggtg
     5341 acgcaggttc tcacggctgt cggaacgacc gaagccatca gtacccagta cgcggtagtc
     5401 gtcagccggt acgtaagtac ggacctgctc agcgaacagt ttcatatagt cggtagatgc
     5461 cactgccgga gcgtcgttca tcacctgagc gatatacgga acgcgcggag tttccagcgg
     5521 atgaagcatg ttccagcgtt cacaatcctg accatcacgc gccagctcgg tgaaggaggt
     5581 cacgctataa acgtcagaac ctacgccgta atctttcgcc aggatctctg ccgcttcacg
     5641 gacgtgacgc aggatagaac cggagcccag cagctgaact ttacctttgc taccttcaat
     5701 ggtttcgagt ttgtagatac ctttacggat accttcctca gcaccttccg gcattgccgg
     5761 catgtggtag ttttcgttca gcgtagtgat gtagtagtaa acgttctctt gtttttcacc
     5821 gtacatacgc tccagaccgt catgcatgat gacagcaact tcgtaagcgt aagccgggtc
     5881 gtaagagata cagttcggga tagtcagcga ctgaatgtgg ctgtgaccat cttcgtgctg
     5941 cagaccttca ccgttcaggg tggtacgacc ggaagtaccg ccgatcagga agccacgcgc
     6001 ttgctggtcg ccagccgccc agcacagatc gccgatacgc tggaagccga acatcgagta
     6061 atagatgtag aacggaatca tcggcagatt gttggtgctg taagaggtcg ccgctgccag
     6121 ccaggaacac cctgcgccca gctcgttgat cccttcctgc agaatctgac ctttctcgtc
     6181 ttctttatag taagcaacct gctcgcggtc ctgcgggatg tactgctgac cgttcgggct
     6241 gtaaatacca atctgacgga acagaccttc cataccgaaa gtacgcgctt cgtcggcgat
     6301 gatcggtacc agacgatctt tgatcgactt gttcttcagc attacgttca gggcacgaac
     6361 gaaagcgata gtggtagaga tctctttgct ctgctcttcc agcagcgcgc cgaagtcttg
     6421 caggctcggc agctcaagct tctcggtgaa gttcggctga cggcttggca gataaccgtg
     6481 cagtttctga cgctgtgcgt gcagataggt atgctcttca gaaccttccg ggaaggtgat
     6541 gtacggcagt ttttcgatat ctgcatcaga caccggcaca ttgaaacggt cgcggatgtg
     6601 acgcacgccg tccatgttca ttttcttaac ctggtgcgcg atgtttttac cttcagccgc
     6661 gtcgcccatg ccgtaacctt taatggtatg agcaaggatt actgtcgctt tgcctttggt
     6721 ttcctgcgct ttcttgaatg cagcgtagat tttcttcgga tcgtgaccac cacggttcag
     6781 tgcccagatc tgctcgtcag tccagtctgc aaccagtgct gcggtttcag gatatttacc
     6841 gaagaagtgt tcacgaacgt acgcaccatc tttcgatttg aaggtctggt agtcgccgtc
     6901 aacggtttcg ttcatcagct ggatcagttt accgctggta tctttacgca gcagttcatc
     6961 ccaacggcta ccccacatca ctttgataac gttccagcca gcaccttcga agatgccttc
     7021 cagttcgttg atgatcttgc cgttaccggt gaccgggccg tcaagacgct gcaggttaca
     7081 gttgataacg aagaccaggt tatccagttt ttcacgggta gcgatggtga tcgcaccttt
     7141 ggattccggt tcgtccattt caccgtcgcc gaggaacgcg taaacggtct gtttagaggt
     7201 atctttcagg ccacggtgtt ccagatattt caggaattta gcctggtaaa tagcaccaat
     7261 cggccccaga cccatagata cggtcgggaa ctgccagaat tccggcatca gtttcgggtg
     7321 cggataggaa gagaggccat tgccgtgaac ttcctgacgg aagttatcca gctgctcttg
     7381 agtcagacga ccttccagga aagcacgagc gtacacgccc ggggagatgt ggccctggaa
     7441 gtaaaccagg tcgccgccat cctgctcgtt gcgtgcacgg aagaagtggt taaagcacac
     7501 atcataaatg gttgcggaag actggaagga cgccatatgg ccgcccagtt cgaggtcttt
     7561 tttcgacgca cgcagcaccg tcatgatggc gttccagcgg atagctgaac gaatacggcg
     7621 ttccagttcc agattacccg gatactccgg ttgttcttca acggggatgg tgttgatgta
     7681 gttgctgata cctgtgcctg cggctacgtt tacaccgcct ttgcgggctt cagcaagcag
     7741 ttggtcgatc agatactgag cacgctcaac accttcttca cggatgaccg attcgatcgc
     7801 ctggagccag tcgcgagttt cgatcggatc cacgtcattt gggaaacgtt ctgacatggg
     7861 ttattcctta tctatctaat aacgttgagt tttctggaac ctgtcccatt gaactctcgc
     7921 cggaaagctc aataagacag gttctacgtt tagttgccgc gcttttatat acgcttgatt
     7981 tacaacatct tctggataat ttttaccaga aaaatcacta attctttcgt tgctccagac
     8041 gacgcagaga acgctcacgg cggctctctt cacgacttct gtcgagcaaa atttcttcga
     8101 taaaggccag atggcgatgc gatgcttcgc gcgcttcttc cggcttaccg gccataatcg
     8161 cttcaaatat gcgggtgcgg tgactactca ccagcggcag catctcgcga cgcgaataga
     8221 gcaattcgaa gttctggcgg acattctggg ccaacatcgg ctccatacac cttagcagat
     8281 gaagcagaac cacattgtgg gccgcttcgg tgacggcaat ctgatactgg agtacggcgt
     8341 ttgattccgc gtccagatcg ccagactgct gcgccagctc tatggcgtgg tggagttcac
     8401 ggatgcgttc cttgtcttca tcggtactac gcagcgcggc gtaataagcg gcgatacctt
     8461 ccagggcgtg tcgtgtttcg agcaagtcat actgtgattc aggatggtcg gagagcagct
     8521 ccaccagcgg atcgctgaag ctttgccata ggctgctctg gacaaaagtg ccgccaccct
     8581 ggcgacgaag caacaagccc ttcgcttcga gacgttgaat cgcctcacgc aaggagggac
     8641 gggagacgtc gaactgtttt gccagttcgc gttccggtgg gagtttttcg cccgggcgga
     8701 gagtgccttc gagaatcaaa aactccagtt gctgctcaat cacatcggag agttttggtt
     8761 ggcggatttt gctgtaggcc atgagttcct gtcttaagcc acttgccgaa gtcaattggt
     8821 cttaccaatt tcatgtctgt gacgctaaag taacaaagta ttcaccttat gtccatacag
     8881 gttttgattg aaatcatgaa actgtgcaca ttttaacaac ttgacatata taacgtttca
     8941 aaattgtaac tatgcacaaa tgtagacttt acgtaggaaa gggatttttt aacgattatt
     9001 aaactataaa aatccgaatt gaaccgattc acttaccaat tttttgattt ttaagtcaat
     9061 aaaaacgaat ttaaattcat tctgcatatt gagaggggtt gaggctgagc tttacaaacg
     9121 gtttcttttt aagcaactca tcttcaacca tgcataaagc gggtgcattc gctgccgcat
     9181 accattattc ttgatctgac ggaagtcttt ttgtaacaat tcaaacttct ttgatgtaaa
     9241 aaaattaata caacaaacgg aattgcaaac ttacacacgc atcactgcgt agatcaaaaa
     9301 aacaatcacc gcacgaggtt tcatgatgga aggtcaacag cacggcgagc agctaaagcg
     9361 cggccttaaa aaccgccata ttcagcttat cgcgctgggt ggcgcgatag ggacagggct
     9421 attcctgggt agcgcctccg taatacagtc cgcagggcca gggattatcc tgggttacgc
     9481 cattgctggt tttatcgcct ttctgatcat gcgtcagctg ggtgaaatgg tggtcgaaga
     9541 acctgtcgca ggctccttta gccactttgc ttataaatac tggggcagtt ttgccggttt
     9601 cgcctctggc tggaactact gggtactgta cgttttagtt gccatggcag agctgactgc
     9661 cgtgggtaaa tacattcagt tctggtatcc ggaaatccca acctgggttt ctgccgccgt
     9721 attctttgtg gtgattaacg ccatcaacct gaccaacgtt aaagtgtttg gcgagatgga
     9781 gttctggttt gccattatca aagttattgc gatagtagcg atgatcatct tcggcggctg
     9841 gctgctgttc agtggcaacg gcggcccgca ggcgagcgtt agcaacctgt gggatcaagg
     9901 tggtttcctg ccgcacggct tcaccggact ggtgatgatg atggcgatta tcatgttctc
     9961 gttcggtggt ctggaactgg tggggatcac cgcagcagaa gctgataacc cggagcaaag
    10021 tatcccgaaa gcgactaacc aggttatcta ccgcatcctg attttctata ttggttcgtt
    10081 agccgttctg ctctcactga tgccgtggac ccgcgttacc gccgatacca gtccgtttgt
    10141 gctgatcttc cacgagttag gcgatacctt tgtggcgaat gcgctgaaca tcgtggtact
    10201 gactgcggcg ctctccgtgt acaacagctg cgtatattgc aacagccgta tgctgtttgg
    10261 tctggcacaa cagggtaatg cgccaaaagc gctggcgtct gtcgataaac gcggcgtacc
    10321 ggttaacacc attctggtgt ctgcgctggt tacagcattg tgcgtattga ttaactatct
    10381 tgctccggaa tccgcatttg gcctgttaat ggcactggtg gtatccgcac tggtgatcaa
    10441 ctgggcgatg atcagtctgg cgcatatgaa gttccgtcgc gccaagcagg aacaaggcgt
    10501 ggtaactcgc ttccctgctc tgctttatcc gctgggtaac tggatctgcc tgctgtttat
    10561 ggcggcggta ctggtgatta tgctgatgac cccaggaatg gcgatttcgg tatacctgat
    10621 cccggtatgg ctgatcgtgt taggtatcgg ctatctgttt aaagagaaaa ccgccaaagc
    10681 cgtaaaagcg cattaatctc tctacgccct cacccatgca gggtgagggc aataatcttt
    10741 acaccaacgc tccgtaaatc gtcagtagcg caatcaccac cacgaccacg aacgaggttt
    10801 tcttcgccat tgaaaccgct gccttcggcg tctccacctt atcgacatgc ggttcacgcg
    10861 ccagagagaa ctgcgccaga cgcgttaaca cctgatactg cgaagtatgg aagtcaccca
    10921 gcgaagcaaa ccaggccggt aacgctttct ccccatgacc gatcaaggca tataccacac
    10981 ccgcaagacg aaccggcacc caatccagta catgaagcac ggcatcaatg ccggactgta
    11041 aacgatgatg cggcgtctgg tatcgcgcca gccagtattg ccatgcacgc aaaaacgcat
    11101 accccatcag cgtaacgggt ccccaggttc cccccacaat caaccagaac agcggtgcaa
    11161 gataaaaacg aaagttaatc cacagcaatg cattttgcag ctcacgcaaa tactcacgtt
    11221 cgtcgcagcc tgccgggacg ccgtgaatca tggtgagttc gccagccatc gtggcacggg
    11281 catggctatc attacgtgaa gcagctgtca gataagcatg ataatgaaga cgaactttac
    11341 ctgcgccaat acacagcaaa ccaatcagca gccacaccag cagcgtggga acgttgaaca
    11401 atactccctg caatgcgcgt aacagtaaaa aagtcacgcc catcgcaata atggtcatgc
    11461 ctaacgtgcg cccgagagaa aaatgtttca cccgccgaaa gaacgcttca agacgatgat
    11521 caagctgcca gtgctcgccc aacttaaaca ggcgctcgaa aattaacacc agtaaggttg
    11581 taaatagcgt catgttgtct ccttgctgac cagcgcacga aaccgtgccc aatcaaatgc
    11641 aggaccggga tcggttttcc gatccggcgc aatatcacaa tggcccgtca tgttattagc
    11701 gatatcggga tagcgatcta tcagtgcccg cgtaaccgcc gcaagctgtt gatactgcgc
    11761 atcggtatac gccagagtat cggtgccttc aagctcaatc ccaatagaaa aatcattgca
    11821 gcgttcacgc ccctgatact gagagactcc cgcatgccaa gcacgtttat cgaaaggaac
    11881 atactggact atttcaccat cacggcgaat caaacagtga gcggagacgc gcaaatgggc
    11941 gatctcagca aagaaaggat gtgcctgcgg atcaatagtt ccagtgaata atgcgtcgat
    12001 ccacggaccg ccaaactcgc ctggcggcag gctaatattg tgcaccacca gcagggtggg
    12061 tgtttcgtca tccgggcggc aatcgtaatg tggtgaggga acgcggcgcg cgccaaccag
    12121 ccacccctgt tctaacaaca tgcaggatct ccttatatgt ggtgctaata cccggttcag
    12181 agtagcatgt ttctacctta tgattcgtta gctatctgga gttttaacat gccgcctcgc
    12241 cgctataacc ctgacacccg acgtgacgag ctgctggaac gcattaatct cgatatcccc
    12301 ggcgcggtgg cccaggcgct gcgggaagat ttaggcggaa cagtcgatgc caacaatgac
    12361 attacggcaa aacttttacc ggaaaactct cgctctcatg ccacggtgag cacccgcgag
    12421 aatggcgtat tttgcggcaa acgctgggtt gaagaggtgt ttattcaact ggcaggcgac
    12481 gatgtcacca taatctggca tgtggatgac ggcgatgtca tcaacgccaa tcaacccttg
    12541 ttcgaacttg aaggcccatc ccgcgtgctg ttaacgggcg aacgcactgc gcttaatttt
    12601 gtgcaaaccc tttcaggagt tgccagtaag gtacgccact atgtcgaatt actggaaggc
    12661 accaacacgc agttgttgga tacgcgcaaa accttacctg gcctgcgttc agctctgaaa
    12721 tacgcggtac tttgcggcgg cggagcgaat caccgtctgg ggctttctga tgccttcctg
    12781 atcaaagaaa atcatattat tgcctccggc tcagtgcgcc aggccgtcga aaaagcgtcc
    12841 tggctgcacc cggatgcgcc agtagaagtc gaagtagaga atctggaaga acttgatgaa
    12901 gccctgaaag caggagccga tatcatcatg ctggataact tcgaaacaga acagatgcgc
    12961 gaagccgtca aacgcaccaa cggcaaggcg ctactggaag tgtctggcaa cgtcactgac
    13021 aaaacactgc gcgaatttgc cgaaacgggc gtggacttta tctccgtcgg tgcgctaact
    13081 aaacacgtac aagcactcga cctttcaatg cgttttcgct aattgctgac agatttatcc
    13141 gctccatcgc gggcggataa tctccccttc cccaactttc ttcgtaacgc ctcgcaaatt
    13201 tcttgtcttt tgctgcaacg aaataaaaaa cctcggaagc tgctttcccg tttcgttttt
    13261 tgcccctcgc ttgtgctgat ggcgtttgcc aaagtagcgc caaccaaatc aaggagcgaa
    13321 acagatggac aagcaacgcg gttttacact tatcgaactg atggtggtta ttggcatcat
    13381 tgccatttta agcgccattg gtattcccgc ttatcaaaac tacctgcgca aagctgcact
    13441 caccgacatg ctacaaacct ttgtgcctta ccgtacagcc gtagagttgt gcgcgctgga
    13501 acatggtgga ttagatacct gcgacggtgg cagcaatggc attccctcgc ctaccaccac
    13561 ccgctatgtt tcagccatga gtgtggcaaa aggcgtggtg tcgctgaccg ggcaagaaag
    13621 tctcaatgga ctaagcgtcg tcatgacacc gggttgggat aacgcaaacg gcgtcaccgg
    13681 ctgggcgcgc aactgcaata ttcaaagtga cagcgcattg cagcaagcct gcgaagatgt
    13741 cttccgcttt gatgacgcca actaaggagc ggcaatgaat attccacagc tcaccgccct
    13801 gtgtctgcgt tatcagggag tcttgctgga tgccagcgaa gaggtggttc atgttgcggt
    13861 agtcgatgca ccttcgcatg agttgctgga cgcattgcat ttcgctacca ccaaacgtat
    13921 tgagatcacc tgctggacgc gccaacaaat ggaaggccac gccagtcgca cacaacagac
    13981 attgcccgta gctgttcagg agaagcatca gcccaaagca gagttgctga ctcgaacgtt
    14041 acaatctgcg ctggaacaac gtgcgtctga tattcatatc gaaccagcgg acaatgccta
    14101 ccgcatccgc ttgcgtatcg acggcgtatt gcatccttta ccggatgttt caccggatgc
    14161 cggagtcgca ttaaccgcca gattaaaagt gctgggaaac ctggatattg cggaacatcg
    14221 cctgccgcag gacgggcaat tcactgtcga actggcagga aacgccgtct catttcgtat
    14281 tgcgacctta ccatgtcggg gtggtgaaaa ggtggtatta aggttgttac agcaggtgaa
    14341 ccaggcactg gatgttaaca cgctgggaat gcagccgtta caactggcgg actttgctca
    14401 tgccttgcaa caaccacagg gactggtgct ggtaactggc cctacaggca gcggcaaaac
    14461 ggtcacgctt tatagtgccc tgcaaaagct gaataccgct gacattaata tttgtagcgt
    14521 cgaagatccg gttgagatcc ccatagccgg actaaaccag acgcaaatcc atccgcgtgc
    14581 cgggctcacc tttcagggcg ttttgcgtgc gttattgcgc caggatcctg acgtcatcat
    14641 gatcggagag atccgcgatg gcgaaacggc agaaattgcc attaaagccg cgcaaaccgg
    14701 tcacctggtg ttgtctaccc tacacactaa ttccacctgc gaaacgctgg tacgtttaca
    14761 gcaaatggga gtcgcccgct ggatgctctc ctcagcgctt acgctggtaa tagcccagcg
    14821 tctggtacgt aaactttgcc cacattgtcg ccagcagcaa ggggagccca tccatattcc
    14881 agtcaatgta tggccgtcgc cgctgcccca ctggcaggca cccggttgtg tacattgcta
    14941 ccacggtttt tatggtcgta cggccttatt tgaagttctg cccataacgc cggtcattcg
    15001 tcagcttatt tccgctaata ccgacgttga atcgctggaa acgcacgcac gacaggcggg
    15061 tatgcgtacg ctttttgaaa acggctgcct ggccgtggag caaggcttaa ccacctttga
    15121 agagttaatc cgcgtactgg ggatgccgca tggcgagtaa gcaactctgg cgctggcatg
    15181 gcataaccgg cgacggcaat gcgcaagatg ggatgctatg ggcagagagc cgtgctttgc
    15241 tgctcatggc actacagcaa cagatggtta ccccacttag cctgaagcga atcgccatca
    15301 attctgcgca gtggcgagga gataaaagcg cggaagtcat tcatcaactg gcgacgctac
    15361 tcaaagccgg gttaacgctt tctgaagggc tggcactgct ggcggaacag catcccagta
    15421 agcaatggca agcgttgctg caatcgctgg cgcacgatct cgaacagggc attgcttttt
    15481 ccaatgcctt attaccctgg tcagaggtat ttccgccact ctatcaggcg atgatccgca
    15541 cgggtgaact gaccggtaag ctggatgaat gctgctttga actggcgcgt cagcaaaaag
    15601 cccagcgtca gttgaccgac aaagtgaaat cagcgttacg ttatcccatc atcattttag
    15661 cgatggcaat catggtggtt gtggcaatgc tgcattttgt tctgccggag tttgccgcta
    15721 tctataagac cttcaacacc ccactaccgg cactaacgca ggggatcatg acgctggcag
    15781 actttagtgg cgaatggagc tggctgctgg tgttgttcgg ctttctgctg gcgatagcca
    15841 ataagttgct gatgcgccga ccgacctggc ttatagtgcg gcagaaattg ctgttacgca
    15901 tcccgattat gggttcactg atgcggggac aaaaactcac gcagatcttt acgattctgg
    15961 cgctgacaca aagtgcaggc attacttttt tgcagggcgt agagagcgtc agagaaacaa
    16021 tgcgctgccc gtactgggtg caacttctga cacaaatcca gcacgatatc agtaacggtc
    16081 atcccatctg gctggcgcta aaaaataccg gggagtttag cccgctctgt ttgcaattag
    16141 tgagaacagg agaggcatcc ggctcgctgg acctcatgtt agacaacctc gcccatcatc
    16201 atcgggataa cacaatggcg ctggcggata acctcgcagc cttactggaa ccgacgttgc
    16261 tgatcataac gggaggaatt atcggtacgc tggtggtggc gatgtatctg ccaattttcc
    16321 atttaggcga tgcgatgagt gggatgggat aacgctggcg tgttgctcca caccagcgtt
    16381 ggaagattac aggttgttga agatgcggtt ttcttgttcc tgcacacgga taaatgtggt
    16441 gcgcttggtc agttctttca ggcgtgaagc cccaacgtat gtacaagctg aacgcaggcc
    16501 gcccaaaata tcacgcgcgg tattttcaac cgggcctcgc agcggcagct taacggtttt
    16561 accttctgct gcgcgatatt ccgcaacgcc gccaacgtga cgtttcatcg cagactcgga
    16621 gctcatgccg tagaacagca taaatttctc gccgttctcc tcaacgatgc gaccgccgct
    16681 ctcttcgtgg cccgccagca tgccgccaag catgacgaaa tcggcaccgc cgccaaaggc
    16741 ttttgcgaca tcgcccggcg tggtgcagcc accgtcgctg acgatcattc cgcccagacc
    16801 gtgcgccgca tcggcacatt cgattaccgc agaaagctgc ggataaccga cgcctgtttt
    16861 gacgcgagtt gtacaaacag aacctgggcc aatgccaact ttaacgatat cagcaccgga
    16921 gaggataagc tcctcacaca tttcaccagt cactacgttg ccagcacaaa tggttttggt
    16981 cggccacgct tcacgcgctt tcgcaacgaa ctgcacgaag tgttcggaat aaccattcgc
    17041 cacatcaata caaacgaagt ttaatgccgg gttcaggtcg agaatctgtt tagttttttc
    17101 gaaatccgca tcagacgtac cggtagaaac catcacatgt ttcagcacat cagcggaaga
    17161 attgttgata aacgcttgcc actcttcgac agaatagtgt ttatgcacag cagtcaaaat
    17221 atcaaaagaa gccagcgcag aggccataga aaatgtacct acggtgtcca tatttgcggc
    17281 gataatcggc acgccggacc agctctgacc tgaatgtttg aaggtgaatt gacgttccag
    17341 ttcaacatcg gaacggcttt taagagtgga gcgtttaggg cggatgagaa cgtctttaaa
    17401 acctaacttc agatcttctt caatacgcat gtgcggattc ctggggttaa tggctataag
    17461 ggtaagagcg caactccagt gacgctatca tacgcactaa tcaatgcggc gcaagactgc
    17521 gaaattgtct ttttttacgc tacaatccca taaatttacc tggtaaatag tgagcgaacg
    17581 tttcatcaat ttcgctttcg tgcactcatt tttaacgttt tgataatcat gatgaattcc
    17641 cgggaatata aattatgagg tatatagttg ccttaacggg aggcattggc agtggcaaga
    17701 gtaccgttgc caatgcgttt gctgatctcg gaattaacgt cattgatgcc gatattattg
    17761 cgcgtcaggt ggttgaacca ggtgcacctg cgctacatgc cattgctgat cactttggcg
    17821 ctaacatgat tgctgctgat ggaacattgc agcgccgggc cttgcgcgag cggatcttcg
    17881 ccaacccgga agagaaaaac tggcttaacg ccctgctgca tccgctgatt cagcaagaga
    17941 cgcaacacca gatccagcaa gctacttccc cctatgtact gtgggttgtg ccattgctgg
    18001 tagaaaactc actgtataaa aaagcgaatc gagtgctggt ggtggatgtc agcccagaaa
    18061 cgcaacttaa gcgcaccatg cagcgcgatg atgtaactcg cgagcatgtc gaacaaatcc
    18121 ttgctgctca ggcaacgcgc gaagcccgcc ttgccgtggc agatgacgtc attgataata
    18181 acggcgcacc ggatgctatc gcatcggatg ttgcccgcct gcacgcacac tatttgcagc
    18241 ttgcgtcgca gtttgtctca caggaaaaac cgtaatgcag acccaggtcc tttttgaaca
    18301 tccactaaat gaaaaaatgc gtacatggct gcgcattgag tttttgattc agcaactcac
    18361 cgttaattta cccatcgttg accacgctgg cgcgctgcat ttcttccgta atgtcagtga
    18421 attactggat gttttcgagc gcggcgaagt ccgcactgag ctgttgaaag aacttgaccg
    18481 gcagcaacgt aaactccaga cctggattgg cgtgcctggc gtggaccaga gccgtattga
    18541 agcattaatt cagcagttaa aagcggcggg gagcgtatta atttccgcgc cgcgtatcgg
    18601 gcaatttctg cgtgaagatc gtttgattgc tctggtgcgt cagcgactga gcatcccagg
    18661 cggctgttgc agctttgatt tacctacatt gcacatttgg ctgcatctac cccaggcgca
    18721 gcgcgacagc caggtagaaa cctggattgc cagcctgaac ccgctcaccc aggcacttac
    18781 catggtgctg gatttaattc gccagtcggc ccccttccgt aaacaaacca gtctgaatgg
    18841 tttttatcag gataacggtg gcgatgccga cttgctgcgc ctgaatctgt cgctcgattc
    18901 acagctttat ccgcaaattt ccggacataa gagccgtttt gccattcgtt ttatgccgct
    18961 ggacactgaa aacggacagg taccggaacg tctggatttc gaactggcct gttgctaagg
    19021 agtaaaaatg tcagaaacta ttacggtgaa ttgcccaacc tgcgggaaaa cggtggtgtg
    19081 gggtgaaatc agcccgtttc ggccattttg ctccaaacgt tgtcagctga tcgacctcgg
    19141 agaatgggct gctgaagaaa aacgaatccc cagcagtggc gatctttccg aaagcgatga
    19201 ctggagcgaa gagccaaagc agtgacattt gtctgatgcc gcacgtaggc ctgataagac
    19261 gcggacagcg tcgcatcagg catcttgtgc aaatgtcgga tgcggcgtga acgccttatc
    19321 cgacctacag acgcttaagc ttcgcaatta ccggttcatt ggctggcgga aaatcatcgg
    19381 cattaagacc gactagcgac atccactcac cgggttgccc ttctttaccc cacggctccc
    19441 cttcccagct ttcgaccagc cagaaccaca gagttatatg cctgtccggg aattcatatt
    19501 ccagtttttc aaatagcgaa aaatgttgtg gggtaatccc gacttcttcc tgaagttcac
    19561 gcactaccgc ctgttccggc gtttcgccca tttcaatttt accgccggga aactccagtt
    19621 tattcgccat gtgcgcatct gctgcgcgac gcgttataaa gatttcattg ttctcgttgc
    19681 gaataatacc taccgcaatt tgcagctttt tcattgtctt aaacctataa aaaaggcgca
    19741 gaatcctgcg ccttttactt caactgttag cttttattgc aggcgaccat ggcactgctt
    19801 gtatttttta ccagaaccgc acgggcaagg atcgttacgt cctactttgc gctcaccggt
    19861 ttgcgccgcc agtgcagctg cggctgcaga gtcgtcatcc tgatggctaa gctgctgcat
    19921 ttgcgctaaa cgctcggctt ccatacgacg ctgttgttcc agctcctcaa cctcttcagg
    19981 catacgtacc tggactttgc tcagcgtgct gataacttca tattttaacg actccagcat
    20041 cgctgcaaac atggagaacg attcacgttt gtattcctgc ttcggatctt tctgtgcata
    20101 gccacgcagg tggataccct gacgcagata gtccatcgcc gctaggtgct ctttccacag
    20161 agagtcgaga gtttgcagca tgacgccttt ttcgaagtga cgcatcatct cagcaccaac
    20221 cacttcttct ttacgctgat acacttcgat ggactgcgcc agaattcgct cacgcagcgt
    20281 ctcttcatgc agttctggtt ctttatccag ccactcggca attggcaaat cgaggtcgaa
    20341 atcgttcttc agacgttcct gcagccccgg aatatcccac atttcttcca gtgactgtgg
    20401 cggaatgtag gcatcaatgg tcgctttgaa cacatcttca cgaatgctgt tgatggtttc
    20461 gctcacatcg ctgacatcca gcagttcgtt acgctgggag taaatggcgc gacgctgatc
    20521 gttagccacg tcatcatatt ccagcagttg cttacgaatg tcgaagttac ggctttcaac
    20581 tttacgctgg gcgttggcaa tcgctttagt cacccacggg tgttcaatgg cttcgcctgg
    20641 cttcataccc agtttacgca tcatgccgga tactcggtcg gaagcaaaaa tacgcatcag
    20701 cgcatcttcc atcgacaggt agaaacggga agaaccagca tccccctgac gaccagaacg
    20761 accgcgcaac tggttatcga tacgacggga ttcgtgacgc tcggtaccga tgatatgcag
    20821 gccacctgct tccagtaccg catcgtgacg tacctgccag tcggctttaa ttttttcaat
    20881 ttgctctgcg gtcggatttt ccagcgcggc aacttctgcc tgccagctac caccgagcac
    20941 aatatctgta ccacgacccg ccatattggt cgcgatagtc accgcagccg gataacctgc
    21001 ctgagcaaca atcgccgctt cgttggcgtg gaatttggcg ttcaggacgt tgtgcttaat
    21061 accggctttg gtcagttcgt ttgacaccag ctccgatttt tcgatggaga tagtacccac
    21121 cagcaccggc tggcctttcg cagtacgttc tttgatatct tcaatgatcg cctgaatttt
    21181 ttccgcttca gtcatgtaga ccaggtccgg cagatcttta cgaatcattg gacggttggt
    21241 cggaacaacg acggtatcca gcttgtagat ggagctaaat tcgaaagctt cggtatcagc
    21301 agtaccggtc atccccgcca gtttttcata cagacggaag tagttctgga aggtgatcga
    21361 agccagcgtt tggttttcgt tctggatctg cacaccttct ttcgcttcca cagcctggtg
    21421 cagaccatcg gaccagcgac ggccctgcat ggtacgaccg gtgtgttcgt caacgatgat
    21481 aacttcacca tctttaacga tgtagtcgac gtcgcgggta aacagcgcat gagcgcgcag
    21541 cgccgccgtt acgtggtgca tcagcatgat gttggccgga gagtacagag actccccttc
    21601 atccatgatg ccctctttca ccagcagttc ttcaatcagc accagaccac gttcggtcag
    21661 gttcacctgg cgagattttt cgtccaccga gaagtggcct tcgccctgga aggtttcgga
    21721 gtcttctttt tcctgacgga tcaggtgcgg aataatttta ttcacgcgtt tatacatttc
    21781 cgagctgtct tctgccgggc cggaaatgat cagcggtgta cgcgcttcat cgatcaggat
    21841 ggagtccact tcgtccacca gcgcatagtg cagtttacgc tgtacacgtt cttcagggct
    21901 gaacgccata ttgtcgcgca ggtagtcaaa gccgtattcg ttgttcgtac cgtaagtgat
    21961 gtcagctgcg taagcttcgc gctttgccgg tgctggcatg cccggcaggt tgataccgac
    22021 agtcaggcca aggaattcaa acagcggacg gttgttttcg gcgtcacgtt gcgccaggta
    22081 gtcgttgacg gtaactacgt gcacgccttt accggttagt gcgttcaggt aagcaggcag
    22141 cgttgcggtc agggttttac cttcaccggt acgcatttcg gcgatgcagc gttcgttaag
    22201 aaccataccg ccgagtaact gaacgtcgaa gtgacgcata ccaaagacgc gcttacttgc
    22261 ctcacgtacc acggcgaaag cttccgggat cagattttcc agcacttcgc ctttttccag
    22321 acgtgcacga aactctgcgg ttttcccttt cagttcttcg tcggagagtt tttccatctc
    22381 cggttccatg gcattgatga tgttgaccac tttgcgcatc cggcgcaggg tgcgatcgtt
    22441 acgactaccg aaaactttag ttaacaattt gattagcata ataaaatctc aaacgccccg
    22501 cgttgcggag ttaataaaat gaagtaaagg tttattgttg ttaggtgagg cgttgagggc
    22561 cagcacggat gccttgcgcc tggcttatcc agacgggcgt gctgaatttt gcttgtgggg
    22621 taaaatgcgc ataatcaatg cgataaccct tttcagacgg cgtgccttcc tgggtcagca
    22681 gcgcgctgag cgtatccagt aatgcaagat gttgcgcctg aagaggcaaa gattcttcag
    22741 caacgggcag tgtttgcggt gccattgcga aagaaagatg acggattacc gtgcgaatgg
    22801 catgttgatg ccagtaatca acggaatagt tcgaattcgg gcggcgtgtg ttcgcttcca
    22861 gcaaggccaa ttgaccaaag ttaactttgg ctgaaggctc gtggttgcgg gttgtcgctt
    22921 ttgcgggcgc gtttggttcg gcggcgttgc tgagcgcagg caaacctaaa ctcgccgcaa
    22981 ccatccctaa taagagatgc ggccagaagt agcgtttacc aaactgtcgc cagcgcgtca
    23041 gtattccact cacgttattg ccatcccgat cgtattcaaa cgtatttagc gcacaaatct
    23101 tatcattaaa gcctgcagtc cacagcacga atgaagataa gttgtgcgga aaagtgctta
    23161 agaaaacagc gttcgcaccg ttttttatag ctcttataaa attcaaaaaa aaatgactgg
    23221 ttcacctggc cggagagagt gccagatttg ccagtcgaat tttatacgac agtataaatg
    23281 tcgttatgcc agtacagctg aaggcgcttt gaaggccaac ggcagttctg cgtcgtcctg
    23341 gaaggtcaca tattcccagg cttcctgttt cgccaggaca gcctgcagca gtttgttatt
    23401 cagtgcatga ccggatttat aagcggtaaa tgcaccaata atattgtgac cacacatgaa
    23461 caagtcaccg atcgcatcga gcattttgtg acgcacaaat tcgtcttcaa aacgcaggcc
    23521 gtcttcgttc agtacgcgat aatcgtcaac aacgatggca caatcgaagc tgccgcccag
    23581 gcacaaacca cgggactgca gatattcgat atcacgcatg aaaccgaacg tacgcgcacg
    23641 gctgatctgg cgcataaacg catcagcgga gaagttcatc gcatagcgct ggttgctgga
    23701 atcaatagcc ggatggttaa aatcgatggt gaaatccagc gaaaaaccat tgtacggctt
    23761 aaattcagcc cacttatcgc catcttcgac acgaacagtc tctttgatgc gaacaaattt
    23821 tttggcgcag ttcaactcgt cgataccggc gtcaagcagc aggtatacaa acggagcggc
    23881 gctgccgtcc atgatcggga tttccggcgc gttaacttcg ataacaatgt tatcgatgcc
    23941 caggcccgcg agagcagcat tgaggtgctc tacggttgaa atccgtacat catgctcgtt
    24001 gaccagacac gtacagagca tggtatcacg cacagatttg gcatcggccg ggaaatctac
    24061 cggtggattc aagtcggtgc gacgatagat gaccccggtg ttggccggcg cagggcgtaa
    24121 cgtcagagtg actttcttgc cggtatgtaa accgacaccc gtcgcctgaa cgatacgttt
    24181 aagtgtcctt tgtttgatca tcgtattatc tcgccaaatt acctatccaa ccgaagtgta
    24241 ctatacattc ggcgggccag tttagcacaa agagcctcga aacccaaatt ccagtcaatt
    24301 cttaatcagc ttgcttacgc aggaatgctg ggatatccag ataatccggc tctttcgcag
    24361 tttgcggcgc attgtcattc acgactttag caaccggctt ctgctcctgg gtcagcggag
    24421 ccatcccatg ctgctggtag cgatccatca ctggctgctg aacctgctta ttggtcacca
    24481 gagtgatttc aggacgtttg tccatgccga tacctgtcgc aacaacggtt acgcgcagct
    24541 cgtcattcat atccgggtca agagaagtac cgataaccac agtcgcgttg tcggaagcaa
    24601 atgcacggat ggtgttacct accgtttcga actcatccag acgcaggtcg aagcccgccg
    24661 tgatgttaac cagcacgccg cgcgcgccag acaggtcgat atcttccagc agcggagaag
    24721 agatagccat ttcagcagct tcttccgcac ggtcttcacc gctcgccacg ccagaaccca
    24781 tcattgcgta gcccatctca gacattacgg tgcgtacgtc tgcaaagtcc acgttcatca
    24841 aacccggacg agtaatcagt tcagcgatac cttgcacagc gcctttcagt acatcgttcg
    24901 ctgcgccaaa cgcatccagc agggagatac cgcggcccag aactttcagc agtttgtcgt
    24961 tcgggatagt gatcagagag tccacatgct tggacagttc agtgatcccc tgctccgcga
    25021 atgccatacg cttcttgcct tcaaagttga aaggcttagt gacgacagca acggtcagga
    25081 tacccaaatc ttttgccact tcagcgacga ctggtgctgc acctgtaccg gtaccaccac
    25141 ccatacccgc agcaataaag accatgtctg caccttccag cgccgcacgc aatgcatcgc
    25201 gatcctcatc agccgcattg cggccaactt ctggattagc gccagcgccc agtcctttgg
    25261 tgataccgct accgatttga atcgtctgtc caaccgctgt tttacgcagc gcttgtgcat
    25321 cggtatttac cgcgaagaat tcaacacctt caatacgctc gcgcaccatg tgttcaacag
    25381 cattaccgcc gccgccgccg acgccgatga ctttaatcac cgcgtcattg gtaagttcca
    25441 ttggttcaaa catagtttct ctccgatttg tgcctgtcgc ctgaggccgt aatcatcgtc
    25501 ggcctcataa aaattaaaac tcttttcgca gccaactatt gagtcgcttg atccacgagc
    25561 caactgatgc tgtaacacgt ttttctactt cagcttcacc gttaagatgt gactctttcc
    25621 catagtgaag caatcccacc gccgtcgaat aatacggctc ctgagcataa tccgttaaac
    25681 cggtaatgtt cagcggcgcg ccgatacgca cttgcgtatg aaacacgcgc tgagcacagg
    25741 ctgcaagacc ttcgatctgc gctgcgccac cggttaatac aatgcctgcc gccaggtgat
    25801 gtttaacccc ttgttggcga agcttttcct gcaactgcaa tatctcttcg ttgaccaggt
    25861 tgagcagctc ggtatagcgc ggctcgatca cctctgccag ggtctgacgt tgcagactcc
    25921 gtggcggacg accgcctacg ctcggcactt ccacgctctc atcttttcca acgatggaac
    25981 ccagcgcaca accgtggcga actttaatcg cttcggcgtc gcttggcggc gtgccaaagg
    26041 cgtaagcgat atcactggtc acgacattgc cagcataagg aattacctta gtgtggcgca
    26101 atgccccacc ggtataaacg gcgatatcca ttgtaccacc accgatatcg acgacgcaga
    26161 cacccagttc acgttcatct tccgtcaata ccgaataact tgatgccagt ccggcaaata
    26221 tcagttggtc aactttcagc ccacaacgtt caaccgcttt gacgatgttt ttcgccatat
    26281 cgttgtgaca tgtgatcagg tgcacttttg cctgcatccg cacgcccgaa agtcctaccg
    26341 gattcttgat cccttcctga tagtcaatcg catactcttg cgggatcaca tgcagcacac
    26401 gatgttcatc gcgcacacgc accgatttcg cggtatggac gacgttttcc acatcttctt
    26461 gcgtcacttc ttcttcagaa ataggcacca taccaatttc attctggcag ctgatgtgct
    26521 taccagaaag cgccagatat accgaagaga tctgacaatc tgccatcaat tctgcctggt
    26581 caatggcgcg ttgtacgcac ttgaccacgg attcgaggtc gttcaccccg cctttatcca
    26641 taccacgtga cgggcagctg cccacgccaa tgatattgac cataccgtcg ggcagaactt
    26701 cccctactaa agcggcaacc ttcgcggtac caatctccag tcctactacc agttttctgt
    26761 ccgtcgcctt gatcattgtt gttctgcctg tgcctgattt tgttgctgag tagattcctc
    26821 tggcggcaag ggcgcccagc ctactgccgc tccagagtca taacgcaaat caacgtagct
    26881 aatccgtttg ccatcggttt gcgcctgctg ctgtaaaacc ggataaagtt ctacaaagcg
    26941 agccaaacgt ttcatcgtat cgccccggcc aagattgagc ttaatatcgt tattcagcgt
    27001 caactgccag gaacgccgcg cggtcatcgc cgcttccttc agagtaaatc tgtcctttgc
    27061 cagcatctgc cccatttcgc gatagccctg caacacttca ttggcgctgc cttccgggcc
    27121 atacagcatt ggaagcacct gcttgctggt gcgatctggc ggcacgctga aggtgtttcc
    27181 ttccgcgtct accatatgtt gatcattcca ccgcgcaatc ggcacatatt caaccagatg
    27241 aatcttcaat tcatcaggcc actgctttct gacgctcacc tgcttaatcc acggcaggcg
    27301 ttgttctatt tgcgtctgga tgatgttgac atcctgggtc ataaaggtac ccggctcacc
    27361 caatgccagg atcgactgcc ggatatcgtc attacgcgtg taatggcgtt caccggtcaa
    27421 caccagcttt gagagcggca ggcgttgcgc atcttccatc cagcccaaca cgacccagcc
    27481 gctcaccaac actgtcgtta aaacggtcag caggaaaagg atccccgcca gacgcgttcc
    27541 attattgcgg cgagaagaaa cctcttcttc gctatttcgc gtgttcagag cagcctgcga
    27601 catattagtc cgccagttcc agaattcgta ctaccaactg cgagaagctc atacctgcct
    27661 gacgtgccgc catcggcacc aggctgtggc tggtcatacc cggtgaggta ttggcttcca
    27721 gcagataaaa ctgtccatcg ctgtccagca taacgtcaat acgtccccat cctttgcaac
    27781 ctaacgtcgt ccatgctttc agcactaatg cctgcaaatt ggcctcttgt gacgcttcca
    27841 gacctgcggg gcagaaatac tgtgtctcat cagagagata cttcgcctca taatcataga
    27901 aggttccgga cggttgaata cgtattgacg gtaaaatttc ttcaccgagt atcgcaaccg
    27961 tgaactccgg cccacttagc catttttcaa tcaatacttc ttcatcgtgc tgaaatgcca
    28021 atcttaatgc atcttgtaga gcattttctg ctactacttt tgacattccc acactggaac
    28081 cttcgcggct cggcttaacg ataaccggca aacccagagc agaaatttct gctaactgct
    28141 tatcgctcag gcctttttca aactctgcgc gggttaacgc tacccacggc gcgaccggta
    28201 aaccggcacc ttgccacagc agtttgctgc gtagtttatc cattgaaagc gcagatgcca
    28261 tcactccgct gccggtataa ggcaagccca tcagctcaag catgccctgc agcgtaccat
    28321 cttcaccgcc gcgaccgtgt agcgcgataa acactttctg aaaacccatc gacttcagtt
    28381 gcgtcacgtc gacttctttc gggtcgacag gatacgcatc aataccgcct tcacgcagcc
    28441 cggctaacac cgctgcgcca gaattcagag aaacttcccg ctcagcggaa gtcccgccca
    28501 acaggaccgc aattttatca gtcatgttgt tcttcctccg gagtttgcgg cttcagtttg
    28561 atttcagcta aagaacgggc aatttttccg atattaccag ccccctgaac gagaatcagg
    28621 tcgttaccga ttaataccgg tgccagcatc tcggctacct gcgcagggtc cggcaccaga
    28681 atgggatcaa ttttcccacg tccacgaatt gtgcgacaca gcgaacggct gtccgctccc
    28741 ggaattggcg cttcgccagc cggatacact tccagcatca acagggtatc aacctgcgtc
    28801 agcacattgg cgaaatcatc atacaggtcg cgcgtacggg taaaacggtg cggctgaaac
    28861 agcattacta ggtttttatc cggccagcct gcgcgcgccg ctttaatggt ggcgtcaact
    28921 tccgtcgggt ggtggccgta gtcatcgacc agcattgccg taccgctttt accattcact
    28981 ggctccagcg ggaattcacc gaggaaatca aaacggcgac cagtcccctg gaagctctcc
    29041 agcgcacgca aaatagcctc gtcgtcaatt ccctcttccg tagcaaccgc aaccgcagct
    29101 gcggcgttca gcgcgttatg acggcctggc gcattcaggg tgacgcgcat cggctctttg
    29161 tcctggcgca gcagcgtaaa gtgcccctgc gggccaatct gctgataatc ttctacacgc
    29221 acgtcggcat cttcgctgaa gccgtaagtc gtggtctgac gtcccacacg cggtaacaat
    29281 tcgcggatca ccggatcatc aacacacatc accgcacgac cgtaaaacgg caggttgtgc
    29341 agaaaattaa taaaagtctg ttttaaattc tcaaagtcgc cctggtaggt atccatgtgg
    29401 tcggcttcga tattggtgac aatcgccacc atcggttgca gatgcaggaa cgatgcatca
    29461 ctctcatctg cttcggcaat caggtaccga ccatgcccca aacgcgcatg aacccccgcc
    29521 gcttttacca gcccgccgtt aacgaaggtt gggtcgagcc ccgcttctgc gtagatgctg
    29581 gaaaccatcg cggtggttgt cgttttgccg tgcgttcctg caatggcgat gccatgacga
    29641 aaacgcatta actcagccag catttcggca cgacggatca ccggaatacg cgcttcatga
    29701 gctgcgacaa tttccgggtt atcggcagaa atggcgctgg aaacaacgac cacgctggca
    29761 tcacgtacgt tttccgggcg atggttgaaa taaatcgtcg ctcccagatt cattaactgc
    29821 tgcgtgaccg gatttggcgc taaatcggaa ccactgatct gataaccttc attggccaga
    29881 acttcggcaa taccgcccat accggcacca ccgatgccga caaaatgtat gtgccgaacg
    29941 cgacgcattt cgggcacgat ggaacgcagt tttgccaatt gttgtgtatt cattctttac
    30001 gccattaact tcttaaattc atacgatgca aaaggcatcg ctacaattac gcccgggcag
    30061 cccggctcac ttcatttgcc actcgctcgg tggcatccgg aatggatgca gcgcgggcgc
    30121 gttctgccat ggttaataag gtttctcgcg accacccggc cagggtgttg gcgacagcat
    30181 ccacgctaag ctgtggctgc tcgataattt tggctgcgcc cgctttttcc agcggtagcg
    30241 cattccagta ttgctggcgg tctttatgtt gaaacggcac aaacaacgcc ggtagtcctg
    30301 ccgcggcgat ttcactcacc gttaaagcac cggagcggca aacgacgaca tccgcccacg
    30361 catacgccgc cgccatatca tcaataaatt ccgtcacttt atgctgcggt tgccccgctt
    30421 cggcatacgc ctgttcaacg gattgttgcg aacctttacc gctctgatgc cagatagtga
    30481 ctgaatcacc cagtttcgca gcaacctgcg gcattgtctg gttaagaatg cgtgcgccct
    30541 gggaaccacc cactaccagc acacgaaccg gaccttcacg tccagccaaa cgttgctgcg
    30601 gcaacggcag cgccagcaca tcggtacgca ccgggttacc caccacttcc gcattaggga
    30661 aagcgcctgg aaacgcctgc atcactttgg tggcaatctt cgccagccat ttattggtta
    30721 agcccgcaat accgttttgt tcatgaagta caaccggaat gcctaacgac cacgcggcca
    30781 gaccacctgg acctgataca tagccgccca taccgagtac cacgtcaggt ttgtacgctt
    30841 tcataatcgc ccgcgcctga cgccaggcgt tgaagatacg cagcggcgca gctatcagtg
    30901 cttttatacc ttttccacgc agaccagaga tacgaatgaa atcaatttcg atgccatgtt
    30961 ttggcactaa gtccgcttcc atacggtcgg cagtccccag ccagcgaact tgccaaccct
    31021 gagccattag atggtgcgca accgccagtc ccgggaatac atgtccaccg gttccgcctg
    31081 ccatcaccat taatcgcttt ccttgaccac tcatcgtgaa cctcgtacaa acgcctgcgc
    31141 tttctccaga cgcgtttcat aatcaatacg caacagcatc atgatggctg tcgacataat
    31201 cagtaagctc gaaccaccgt aactgatcag cggcaatgtc agacctttgg tcggtaacat
    31261 ccccgccgcc gcgcctacgt taaccagcgc ctggaagcta aaccagatgc caatagaaca
    31321 ggcgagaaaa ccggaaaaac ggtggtcaat ttctaacgct ttgcggccaa tcgacatcgc
    31381 gcgaaaagcg acgaagaata ccattaaaag tgccagcacc acaccgacat accccagttc
    31441 ttcgccgata atggcgaaaa taaagtcagt gtgcgcttcc ggcagatact ccagtttttg
    31501 taccgagtta cctaaacctt gtccccaaag ttcgccgcga ccaaacgcca tcagcgattg
    31561 cgttaactga tagccgctgc caaagggatc ttcccacggg ttccagaatg cggtaacacg
    31621 gcggatacgg tacggttcgg cgagtatcag caacacaacc gctgaaatgc ccataccgat
    31681 aatggcaatg aactgccaca atttcgctcc cgccaggaac aacatcgcca gcgtagtcac
    31741 aaacaacacc accaccgtac caaggtctgg ctgtgccagc agtaacactg ccaacaccag
    31801 aatcacgccc atcggtttca ggaagccgcg caggttatta cgtacttcgt cgcctttacg
    31861 caccagatag ttggcgatat aacaaaacag cgacagtttt gtcagctccg caggctggat
    31921 acgcagcaaa ccgagatcga tccaacgcga tgccccttta accgagctac ccactaccag
    31981 cacgatcatc agcaggatga tagatccgag cagcatcgtg gcactgtagc gttgccagaa
    32041 ctccatcggc agacgcagcg taatgatcgc cagaatgaat gccaggatca gatagacgcc
    32101 atcacgcttc gcgaagaaga acggatcgtt ggttaagcgt tgccctatgg gcattgacgc
    32161 cgaggtcacc atgataaagc caatcgccgc aaggccgaag gtcagccaca gtaaggtgcg
    32221 atcgtacatg atcaggctgt cggtatcttt ttcccgcgag cccatcaccc agccttttag
    32281 cgccgtggag atccagacca ggatactgaa tcctggcagg cgcggcattt tcaggcgagg
    32341 gagagataaa cgcatcaacc taactccttc gccagacggg caaactcatt gcctcgttgt
    32401 tcaaagttct tgaactgatc aaggctggca caggccgggg agagcagaac catatcgccc
    32461 agctgaacac gcggagccag caagcgcatc gcttgttcca tggtttcggt ttgttctgcc
    32521 acttccgggc gtagcgccgc cagctgcgca ccgtcacgac caaaacagta caaacgcacg
    32581 ttgtcgccat tcagatagcg cgccagtgga ctgaagtccg ccgatttacc atcgccgccc
    32641 agcaacaaat gcagcgtgcc gtctacctgc aagccattca acgccgcttc cgtactgccg
    32701 acgttagtgg ctttcgaatc gttaatccag cgtacgccat tgtgctccag caccacttca
    32761 aagcgatgcg gcagaccggt gaaggtggtt aacgctttca ggctgctggc gcgcggcaaa
    32821 ccagctgcgt ctgccagtgc cagtgctgcc agcgcattgg tgtagttatg ctgcccggaa
    32881 agtttcatct ctttcacatt cagcactttc tcgcctttaa cccgcagcca ggtttcgccc
    32941 tgctgatgat tcaggtgata gtcacccatg ttgacgccaa agctgacgca gcgttcatca
    33001 gcaccgcgaa tcggcattgt taaggcatca tcagcattga cgacacaaac tttcgcattt
    33061 tcgtaaatac gcaattttgc cgcacggtat tgctgcaaac caaacggata gcggtccata
    33121 tgatcttcag tcacgttcag aatggtcgcc gccaccgcct gtaagctgga agtggtttcc
    33181 agctggaagc tcgacagttc cagcacgtac agttcgcact cagcatcaag cagcatcaac
    33241 gctggcaggc caatattgcc acccacacca acgttaaccc ccgccgcttt cgccatttca
    33301 cccactagcg tggtgaccgt gcttttgccg ttagaaccgg taatcgccac aatcggtgct
    33361 tgtgcttcgc gacagaacag ctcgatatcg ccaacgattt cgattccggc atcagcggca
    33421 gcgcttaagg atggatgcgc ctgtgcaata ccgggactgg cgacaatcag atctgccgcc
    33481 atcagccatt catcattcag actgcccgtg tggcgttcta cggcttcggg taatttatcc
    33541 aggccaggcg gtgtcatacg cgtatccata acgcgcggcg tcacaccgcg agcgaggaaa
    33601 aagtccacgc aggaaagccc ggtgaggccc aggccgataa tgacgacatt tttaccctga
    33661 taatcagcca tgattaacgt accttcagcg ttgccagacc aatcagaacc agcatcagcg
    33721 aaataatcca gaaacgcaca atgacgcgcg gttccggcca gcctttcagt tcatagtggt
    33781 gatgaatcgg tgccatgcgg aaaatacgtt gtccgcgcag tttaaaggag ccgacctgca
    33841 ggatgacaga aagcgtttct accacgaaca cgccccccat aatcaccagc aggaattcct
    33901 gacgtagcag tacggcgata atgcctaacg caccacctaa cgccagcgaa cctacatcgc
    33961 ccataaagac ctgcgccgga taggtgttaa accacaggaa gcccagtcct gccccgacta
    34021 tcgccgtgca gacaatgacc agttcccctg cgtgtcgcag atacggtata tgcaagtagc
    34081 tggcaaagtt catattgccg gtcgcccacg ccaccagcgc aaaaccaccg gcgacaaata
    34141 cggtcggcat aattgccagg ccgtcgagac catcggtcag gtttaccgcg ttgccagtac
    34201 ccacaatgac gaagtaagcc agcagaatgt agaacagccc cagctgcggc atcacatctt
    34261 taaagaatgg gaccaccagc tgcgttgcgg gcgtgtcttt gccggcaagg tacagggcga
    34321 aggcgacacc cagcgcaatg accgacatcc agaaatactt ccaacgagcg atcaaccctt
    34381 tggtgtcttt acgcaccact ttgcgataat catcaacaaa gccaataaca ccgtaaccta
    34441 ccagcaccac caacacgcac cagacgtacg gattggacgg gtaagcccac agcagtacgg
    34501 agatcacaat cgccgtcagg atcataatcc cgcccatggt cggcgtaccg cgcttgctga
    34561 agtgtgattc aggaccgtcg ttacgcacca cctgaccaaa ggaaagtttt tgcaaatgag
    34621 ctatcatacg cgggcccatc cacaatgaga tgaacagcgc ggtcagcagg ctgacgatgg
    34681 cgcgaaacgt cagataggaa aagacgttaa agccggaata atatttgacc aaatgttcgg
    34741 ccagccaaac taacatgtcc cattctcctg taaagcgcgt actacctctt ccatggcggc
    34801 actacgtgaa cccttaacta aaatcgtaat tacctgttgc tcagcaatca gtgatttaag
    34861 acgcgtaatt aacgcagttt tatcagcaaa atgttcgcca acgccgctgg cggtgctgat
    34921 agcatggctt tgtttaccca cgcttaacac gcggtcaata ccagcagctt ttgccgcctc
    34981 gcccacctgt acatggcagg cttcgctttc agcgcccagt tccgccatat cgcccaccac
    35041 cagcacgcgg tagcccggca tttcagccag tacctgaaca gcggcggtca ttgaaccgac
    35101 attggcgttg taggagtcgt cgagcagcaa ctggttttct gccagttgga tggggaacag
    35161 acggcctgga acagctttca gatttgccag ccccgctttg atagcattaa gcgttgcgcc
    35221 cacggacatg gagagcgcag cggctgccag cgcattcgca atattgtgac gccccggcaa
    35281 cggcagcaga acatcgacgc taccggttgg ggtttgtagg gtaaattccg taccgtgcga
    35341 ggtcacatgg atattggtgg cggtgaaatc gctgttggcg gcattgggtg agaaacgcca
    35401 cactttgcgt gagccaatta cgctctgcca gttcagccag tcgttgttgt cggcgttcat
    35461 aatggcgata ccgttttccg gcaggccgct aaagatttca cctttcgctt tcgcgacacc
    35521 cgcaagcgag ccaaaacctt ccagatgcgc cgctgccagg ttgttgacca gcgcagcttc
    35581 cgggcgagtc agactcacag tccaggctat ttcgccctga tggttcgcgc caagttcaat
    35641 aactgcgtaa tcgtattccg gcgttaagcg caacaacgtc atcggtacac cgatgtcgtt
    35701 gttgagattg cctgccgtat aaagcgtgtt gccgcactgg cttaaaatcg ccgccgtcat
    35761 ctctttaacg gaggttttgc cggaggaacc tgtcagagca accacgcgcg ccggaacttg
    35821 ctggcgaacc catgcagcca gttcaccaaa cgccagacgc gtatccttga cgattaactg
    35881 cggcaggtcg atatccagcg gacggctgac cagtagtgcg cccgcgccgc cagctttcgc
    35941 ctggtcggca aaatcatgag catcgaaacg ttcgcctttc agggcaacaa acaggcagcc
    36001 cggcgtcagt tttcgggtat cagtggttac agcatcaagg gtgatatctg caccttgcag
    36061 ttcaccgttg agaatgtcgg taagttggct aagggttacg ctaatcatgc aatcaccccc
    36121 agcagacgcg ccaccgtgac gcgatcggag tagtccagac gctgattgcc aacaatctgg
    36181 taatcttcat ggcctttgcc cgcgaccagt accacatcat tctctttagc ctgcataacg
    36241 gcgcaagtca ccgcttcagc acggccttcc atcactttgg catgtccggc atctaacatt
    36301 cccgccagaa tatcgttgat gatggcacgc ggttcttcgg tacgcgggtt atcgtccgtc
    36361 accaccgcca cgtcagcaaa ctcttcggca attgcgccca tcagtggacg cttaccttta
    36421 tcgcgatcgc caccacagcc aaagacgcac cacagcttgc ccgcacagtg caggcgcgcc
    36481 gcctgtaagg ctttttccag tgcatccggc gtatgcgcgt aatccaccac caccgtcggt
    36541 ttgcctggcg cagtgaacac ttccatacgt ccgcaaaccg gttgcagacg cgcggcggtt
    36601 ttcagcagat cagccagtgg atagccgagt gccagcagtg tcgccagcgc gagcagcagg
    36661 ttgctgacgt taaaagcgcc catcagatgg ctttcaattt cgccatcgcc ccaacttgag
    36721 ctaaagcgaa tcgtcgcacc gctgtcgtga tagttcacgt cgatcgcttt taaccagcgt
    36781 ccgtgacagt tcggattaat atgatcttcc attgataccg caaccgcgtc cggcagtttt
    36841 gccagccagc ggcggcccac ttcatcgtcg gcgttaataa tcgcctgacc gcaatgatgt
    36901 tcagaataaa gcagccattt cgcagcttcg tagtgttcca tatcaccatg ataatcaagg
    36961 tgatcgcggc ttaagttggt aaagaccgac gccgcaaatt tcaatgccgc cacacggtgc
    37021 tgtaccagcc cgtgggagga aacttccatt gcgcaaaacg tcgcgccctg ctccaccagc
    37081 cccgccagct catgctgaac atcgactgcg gaaccggtag tattttctgt cgggatcact
    37141 ttcccaagca gaccgttacc aacggtgccc attaccgcgc tggtttcgcc aagcagttgg
    37201 ctccactgcg ccagcagctg ggtagtcgtg gttttgccgt tggtgcccgt tacgcctacg
    37261 agacgtaaat tgtcagaggg ttcatggtaa aagcggcccg ccagtgcaga taaacgctca
    37321 ttgagctggc tgagatagat gaccggtacg ccgtgcattt cacggatttc accgtcggtc
    37381 gcctcatctt tcgctttcgc ctctgcaata atggcagcca caccttgcgc tatcgcctgc
    37441 gggatatatc gacgcccgtc cgcctgatga cctactacag ctacaaagag atcgcccgcc
    37501 gcagccacac ggctgtcgag tgtcatctct cgcagtgctc gcgaaggtgc gtctggcacc
    37561 cacggagcaa gaaggtcgcg caaattacga tctgccacct gtcccctcgc cttgattaat
    37621 cacaaattca tttttatcgc ccgttgtcag cgcatccggc tcgatgttca tggtacgcaa
    37681 tacgccgccc atgatggcac caaagaccgg cgcggaaacg gcgccgccgt agtatttacc
    37741 cgcctgcgga tcgttgataa caacaaccag cgcgaagcgc ggctgactcg caggcgcaac
    37801 gcctgcggta taagcaatat atttattgat gtagcgaccg tccggcccga cctttttcgc
    37861 ggtaccggtt ttaatggcga tacgatagcc tttaatcgcc gccttcacgc cgccgccgcc
    37921 tggtagcgcc acgctttcca tcatatgcac cacagtgcgg acaatggatc ccgggaagac
    37981 acgttcaccg ggaaccgggg ggtcaacttt ggtaatcgac agtgggcgat aaatgccgta
    38041 gctgccgata gttgcgtaga ctcgcgctaa ctgtaacggt gttaccatta gcccgtagcc
    38101 gaaagagaag gtggccctct ctatgtcaga ccaccgttgt ttttgaggat ataagccact
    38161 gcgttctccg accaacccca aattggtcgc ttttcccagt ccaaaacgtg agtaagtatc
    38221 tactaacgct gaggacggca tcgctaacgc cagcttggaa acaccgacgt tactcgactt
    38281 ctgtaatacc ccggtcaggg ttaattcgct gtagcgtgcc acgtctttga tttcgtggcc
    38341 gttaattcga taaggaatgg tattgagtac cgagttttcc cgcaccacgc cacgttgcaa
    38401 cgcggtcatt accaccatcg gtttaaccgt tgagcccggt tcaaacacgt cggtgatggt
    38461 acggttacgc atcgcctctt tcggcgtgcc gctcagattg ttagggttgt atgacgggct
    38521 gttagccatc gccagcactt caccggtgtt gacatccacc agcacggcgc taccagattc
    38581 agccttgtta aaggccaccg cgttgttcag ttcgcgataa accagcgcct gcaggcgttc
    38641 atcaatactc agcgccaggt tgtgcgctgc ctggctgtca gtagaagaaa tatcttcaat
    38701 tacgcgacca tagcggtctt tacgcacaat gcgctcaccc ggctgcccgg taagccattt
    38761 atcgaaactc ttctcaacgc cctcaatccc ttgactatcg acgttagtaa agccgatgag
    38821 gtgagcagtc acttcgccgg acggatagta acggcgagac tcttcacgca gatgaatccc
    38881 cggcagtttc agttttttga tgtagtccgc catgtcaggg ttcacctgac gcgccagata
    38941 aataaagcgc cctttcgggt tggcgttaat gcgggctgaa agctgatcca gcggaatatt
    39001 gagcgcgtta gccagcgcct tccagcggtc accgacgctg ataccgccag cgtcatgcac
    39061 ttctttcggg tcagcccaaa tcgcttttac cggcacgctc accgctaacg ggcgaccaga
    39121 acggtcagta atcatgccgc gggaggtgga aacttgctga acgcgaagag aacgcatgtc
    39181 gccctctttc accagcatat ccggggagat aacttgtaac cacgctacgc gtccgagcag
    39241 aaaagccagc gccaggagaa tacagccgca taacaacgca aaacgccaac tgataaagtt
    39301 ggcatgttct tcctgacgtt ttggtttctg cgttttcgcc gctgctttca tgcgtcgcgt
    39361 ttatccttat ttttgcacta cgatattttc ttgtgacgga tcaacatgct gcatttgcag
    39421 cttttccgtg gcgatccttt ccacccggct atggtcgccg agcgcattct cttcaaggat
    39481 caggttgcgc cattcaatgt ctaaagcatc tcgctccagc accagttgtt cgcgctgagc
    39541 ggtcagtaaa cgggtatggt gcgccgtggt taccacagtc accgccgtca aaataatgca
    39601 aatgaacagg cagagtggca gcttcccaaa tcgcaaaaga tcgtcaccga taacaccagg
    39661 caatgcatgg cgctcgtggc ttcccatcga tcctttaact ttgcttagag cttctgtcac
    39721 tctgctgatc atgcattcgt cctctctgca atacgcagaa ctgaactacg ggcacgaggg
    39781 ttctcagcca cctcttcttc gcccggcatt aacttgccta gtgctcgcag ctgacggcca
    39841 cccagttttt tgagctgctc ttcagtcatc ggtaaccctg ccggaacttg cggaccgcgg
    39901 ctgttttcac gcataaaacg tttcacaata cggtcttcca gcgagtggaa gctgatgatc
    39961 gaaagccgcc cacccggggc cagcacgttg agcgagcttt ttagcgcctg ctctatctcc
    40021 tccagttcac tgtttaccca aatgcgcacc gcctggaagg tacgggtcgc gggatgttta
    40081 aacttatctt tcaccggcgt tgcaacagcc acgacttccg ccagttcttt ggtgcgggtc
    40141 atcggctgtt cgcggttacg ctcgacaatg gcgcgggcaa tgcgtttggc aaaacgctct
    40201 tcaccatagg ttttcaatac ccaagcgata tcggcttctt ctgcggtttg tagccattca
    40261 gcggctgact gcccacgggt tgggtccata cgcatgtcca gcggaccatc gcgcataaag
    40321 gaaaagccgc gctcagcatc atcaagttgc ggtgaagaga cgccaagatc gaggagaatg
    40381 ccgtcgatct tgccgataag atcgcgctcg gcaacgtatt cgcccagcgc ggagaaaggt
    40441 ccgtggatga tggagaagcg cggatcatca atagtcttcg caacggcgat agcctgcggg
    40501 tcgcgatcga tcgccagcaa acgcccctct tcgccaagct gcgagaggat cagacgtgag
    40561 tgaccaccgc gaccaaaagt cccatcaatg tagatgccat caggacggat attgaggcca
    40621 ttaacggctt catccagcag caccgtagta tgtttatagt tttccatcat tttatagaga
    40681 caagtcctgc agtcgctccg ataagtctcc ggtagccaac tgctctgcgt cgatatcttc
    40741 cttgacctgt tgatgccagg ttgtttcatc ccacagctca aacttgttga actgtccaac
    40801 cagcatcact tcttttgtca gcccggcatg ttgccgcagt actggcgcga ttaacaatcg
    40861 acctgcgcca tccatctgac attcgctggc atgacctaac agtaggcgct gcacacggcg
    40921 ctcaaccggg ttcatgctcg acagacgcga taatttttgc tcgataattt cccattcagg
    40981 cagggggtaa agcagcaggc acgggtgatg aatgtcaatg gtgcaaacca tttgaccgac
    41041 agcgttctca agcagctgtt cccggtaacg ggtaggcact gataagcgcc ctttgctgtc
    41101 gagattgact aacgttgctc cccggaacat gccagcctca ccccttattc ccactttgcc
    41161 ccacaatttc ccactgaaag gagtttacgg agcggaggaa aagcttgtca agccagtcac
    41221 aaggcatacc gaggtaaaaa acccgatgtt tacggctaat atcagcgttg tttattttac
    41281 gtccaacgtg cgcagcaaat taacgttatg aatagctgta agaaaaaatc cctcatcgtc
    41341 atcaccgttt taaaatgact cactattttc cacagcaaat ataaagtgtc agtttgcgac
    41401 gcgagcggca ttttaggaca tatcttcccc ggttaacagt ccctgttgcg tctgtgtggc
    41461 gcttgatgcc agcgggtttg cgcaaaagca aagaaaagtg cttgttaaat cggcaattca
    41521 tctctgcatg taacaaaata atgcaaaaac gcgctgtcga tatcacttaa ttattgtgaa
    41581 tttcagctgg tgagttaatt aacgggcggg tttaaaagaa cttatcaata atagtcagga
    41641 atagtcttat ttactttaag gatattgatg tccattcccg tactctacg
//
LOCUS       DAESHI010000026        41145 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.026, whole genome shotgun sequence.
ACCESSION   DAESHI010000026 DAESHI010000000
VERSION     DAESHI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41145
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.026"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(227..1084)
                     /gene="murI"
                     /locus_tag="KW011_001429"
     CDS             complement(227..1084)
                     /gene="murI"
                     /locus_tag="KW011_001429"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBH7659809.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(1029..2873)
                     /gene="btuB"
                     /locus_tag="KW011_001430"
     CDS             complement(1029..2873)
                     /gene="btuB"
                     /locus_tag="KW011_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HBH7659810.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     regulatory      complement(2938..3128)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3242..4342
                     /gene="trmA"
                     /locus_tag="KW011_001431"
     CDS             3242..4342
                     /gene="trmA"
                     /locus_tag="KW011_001431"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HBH7659811.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4382..4741)
                     /locus_tag="KW011_001432"
     CDS             complement(4382..4741)
                     /locus_tag="KW011_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HBH7659812.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4741..5391)
                     /gene="fabR"
                     /locus_tag="KW011_001433"
     CDS             complement(4741..5391)
                     /gene="fabR"
                     /locus_tag="KW011_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HBH7659813.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            5712..7112
                     /gene="sthA"
                     /locus_tag="KW011_001434"
     CDS             5712..7112
                     /gene="sthA"
                     /locus_tag="KW011_001434"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HBH7659814.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(7095..8012)
                     /gene="oxyR"
                     /locus_tag="KW011_001435"
     CDS             complement(7095..8012)
                     /gene="oxyR"
                     /locus_tag="KW011_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HBH7659815.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(8279..9652)
                     /gene="argH"
                     /locus_tag="KW011_001436"
     CDS             complement(8279..9652)
                     /gene="argH"
                     /locus_tag="KW011_001436"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBH7659816.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(9713..10489)
                     /gene="argB"
                     /locus_tag="KW011_001437"
     CDS             complement(9713..10489)
                     /gene="argB"
                     /locus_tag="KW011_001437"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBH7659817.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(10497..11501)
                     /gene="argC"
                     /locus_tag="KW011_001438"
     CDS             complement(10497..11501)
                     /gene="argC"
                     /locus_tag="KW011_001438"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBH7659818.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            11655..12806
                     /gene="argE"
                     /locus_tag="KW011_001439"
     CDS             11655..12806
                     /gene="argE"
                     /locus_tag="KW011_001439"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HBH7659819.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            13158..15809
                     /gene="ppc"
                     /locus_tag="KW011_001440"
     CDS             13158..15809
                     /gene="ppc"
                     /locus_tag="KW011_001440"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HBH7659820.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            15992..17725
                     /locus_tag="KW011_001441"
     CDS             15992..17725
                     /locus_tag="KW011_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HBH7659821.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            17940..18791
                     /locus_tag="KW011_001442"
     CDS             17940..18791
                     /locus_tag="KW011_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH7659822.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(18778..19119)
                     /locus_tag="KW011_001443"
     CDS             complement(18778..19119)
                     /locus_tag="KW011_001443"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBH7659823.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(19121..19999)
                     /locus_tag="KW011_001444"
     CDS             complement(19121..19999)
                     /locus_tag="KW011_001444"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HBH7659824.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(19965..22261)
                     /locus_tag="KW011_001445"
                     /pseudo
     CDS             complement(19965..22261)
                     /locus_tag="KW011_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            complement(22312..22632)
                     /locus_tag="KW011_001446"
                     /pseudo
     CDS             complement(22312..22632)
                     /locus_tag="KW011_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            complement(22647..23726)
                     /locus_tag="KW011_001447"
     CDS             complement(22647..23726)
                     /locus_tag="KW011_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBH7659825.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            24035..26536
                     /gene="ptsP"
                     /locus_tag="KW011_001448"
     CDS             24035..26536
                     /gene="ptsP"
                     /locus_tag="KW011_001448"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBH7659826.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            26548..27210
                     /gene="fsa"
                     /locus_tag="KW011_001449"
     CDS             26548..27210
                     /gene="fsa"
                     /locus_tag="KW011_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBH7659827.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            27221..28324
                     /gene="gldA"
                     /locus_tag="KW011_001450"
     CDS             27221..28324
                     /gene="gldA"
                     /locus_tag="KW011_001450"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HBH7659828.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            28598..29215
                     /locus_tag="KW011_001451"
     CDS             28598..29215
                     /locus_tag="KW011_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HBH7659829.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(29242..30147)
                     /gene="yijE"
                     /locus_tag="KW011_001452"
     CDS             complement(29242..30147)
                     /gene="yijE"
                     /locus_tag="KW011_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HBH7659830.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(30241..32421)
                     /gene="katG"
                     /locus_tag="KW011_001453"
     CDS             complement(30241..32421)
                     /gene="katG"
                     /locus_tag="KW011_001453"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HBH7659831.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(32750..33640)
                     /gene="metF"
                     /locus_tag="KW011_001454"
     CDS             complement(32750..33640)
                     /gene="metF"
                     /locus_tag="KW011_001454"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBH7659832.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(33989..36421)
                     /gene="metL"
                     /locus_tag="KW011_001455"
     CDS             complement(33989..36421)
                     /gene="metL"
                     /locus_tag="KW011_001455"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HBH7659833.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(36424..37584)
                     /gene="metB"
                     /locus_tag="KW011_001456"
     CDS             complement(36424..37584)
                     /gene="metB"
                     /locus_tag="KW011_001456"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBH7659834.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAVGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            37861..38178
                     /gene="metJ"
                     /locus_tag="KW011_001457"
     CDS             37861..38178
                     /gene="metJ"
                     /locus_tag="KW011_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HBH7659835.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            38362..38970
                     /locus_tag="KW011_001458"
     CDS             38362..38970
                     /locus_tag="KW011_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HBH7659836.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            39115..39790
                     /locus_tag="KW011_001459"
                     /pseudo
     CDS             39115..39790
                     /locus_tag="KW011_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(39963..40199)
                     /locus_tag="KW011_001460"
     CDS             complement(39963..40199)
                     /locus_tag="KW011_001460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659837.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFLSNSDSAALYCVAKIF
                     PDGENILIYKVNIINASSKKDVFFMKAQHQPTMT"
     gene            complement(40201..41112)
                     /locus_tag="KW011_001461"
     CDS             complement(40201..41112)
                     /locus_tag="KW011_001461"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HBH7659838.1"
                     /translation="MKKEHADGSVTQSQFDAVGRLRTQTDAAGRTTEYSPDVVTGLIT
                     RITTPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGNLLNEENPHQLQQLIRLPG
                     QQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYTYPLSPVNSMDPLGLYEFK
                     SKNIDDIGIFALAMCNGESINENKEYGGLICKKQGEYFPMNPISSNDNDSVDLRNIKC
                     PEGSERVGDYHTHGFYSDDKGNKVTKENDVYDSLNFSSKDLTNSYMNGMGKKEYSSYL
                     GTPNNTYLKYNPKAKGNGVTIIRQGSN"
BASE COUNT        10063 a        11316 c        10249 g         9517 t
ORIGIN      
        1 ccgcttcgct ttttctcagc ggcgcgaggt gtgcataata cgctttcccg ctacagagtc
       61 aagcattttt tttgcttttc tctgttgaga ttctcaggag aaccccgctg acccggcggc
      121 gtgtttgccg ttgttccgtg tcagtggtgg cgcattatag ggagttattc cggcctgaca
      181 agaggaaatt taaaataatt ttctgaccgc gcaacattca accaaatcag cctaaaactg
      241 ccagtttttc gagcgtttcg aagccgtaac gctgtaaaac gggcaataat tgttcagctt
      301 ctggcgtcat tgccatacaa aaggcaatat tcgcatcggc agattttgca tccggggctt
      361 catgttctaa caaccaggcc gttcggcgag caatcgctgc gccagaatcc accagccggg
      421 ttccctctgg cagcacttgt aacagttctt cttgtagtag agggaaatgg gtgcaaccca
      481 atacaacggt atctggcggc tctttcattc ttaaccacgg gcgtaggata cgttttagtg
      541 catccagaga aacatcttcg ccatgtagct tcgcttcagc caactcaacc atctctgccg
      601 agcccagcat ttctatctgg cattcattag cgaaacgcgc gatcagctca tgagtataag
      661 aacgtttaac tgttccgcgg gttgccagta atccgacaat gccatttgcc gtcagacgtg
      721 cagcaggttt aatcgccggc acgacaccaa caaccgggaa gtcgaacttt tcgcgtaatg
      781 caggaagtga aacggtactg gcagtgttgc aagcgaccac agccagcgca aggggataac
      841 gttcttgcac cgcggtgaca attgccacca ctcgctcaac aataaacgct tcgctttttt
      901 cgccatacgg gaaagcgacg ttatcgaaag cataaatgta atggagatcc ggtaagagat
      961 gccggatctc gtcatagacc gacaacccac cgacgccgga gtcaaacacc agcacggtgg
     1021 gacgtggttc agaaggtgta gctgccagac aaggtgtatt cccgtcctgc agtttggtag
     1081 ccatagactg tctcataatc tttgtcgaac aggttggcta ttttaccacg aactgtcagg
     1141 tgagaggtga ccggatacgc aaccgcaaga tcccacaagc tcacaccgcc cattttaacg
     1201 gtttgataag gataagatga gtaatcctta tcatagcgag tgcctaaata ctgataagta
     1261 ataccccagt cgaagtcata caactgccag tcgagctggt atttcacctg ctgtttagca
     1321 cggcgtaaca acggcgtgtc ggtaatcgca ttgcgcgcat cgacataatc ataactcaca
     1381 gtatgcgtca gtggtccggt atcaaaattg gcggtcgcct cgacgccctt aatccgcgct
     1441 ttcccttcgt tgtaatattt cagggtgtga tcatcataat cgatcaagtc actgacatcg
     1501 ttacgatatc cggaaatacg ccagttcacc ccagcggtta agccttcaaa cgcgccttcc
     1561 cactgtttgc ttttctccgg gtccagattc ggatttccgt agaagccata cagttgcccc
     1621 agatttggtg ccttataaga tgtcccgtag gaagcaatga agcgataacc ttcgatgaat
     1681 tcccaaccgg cgctggtttg ccaggttcca tgacgaccaa actgtgagtt atcgtcactg
     1741 cgtgctgcgc cttcaaaggt aaaatcgccg acttgttgca gcccggtcag atagatgccg
     1801 gtattacgtt gatcatatcc atcctcaaca taacctgtac ccggcgtcgt agtctgtttc
     1861 tgccagtcga cacccgcacc aatactaccg tgaccaacga tgacattgtt tgcccactgg
     1921 acggtgtatt gcttcatctc atcgagcgtc gccgacgaat cataacgacc ataatgggga
     1981 tcgtagttgt aatctttgct atggctatag ctggtaatga gttgtgattt aatcagttcg
     2041 ccgttatagc gcagcccggc gtcccaactt tggctataga gtttacgggt atcgagcaac
     2101 ggtgaaccgg gagaataata cgcgtcataa ttggtacggt tatcatagcc atagccgcgc
     2161 acaaagccgc tccaggcatc agtaaagtta tgctccagcg cgccataaag cgttttactt
     2221 aaaaaaccat cgttatctgt ctgcgcttgc gttcctgtat taccataggc aacaacatca
     2281 taaccatgag tatgggcata atcgcccaac agcgttaccc gtgtcttatc ccccagttgt
     2341 tgctgcgtag agacatcata gttctgataa ctattgcttc cccaccctgc tgaaatttcc
     2401 gttccgggtt catcgcgcgt cgtgatgata ttcaccaccc cgcctattgc atcggaacca
     2461 taaacagcgg agcgcggccc acggatatat tcaacacgct ggacaagcgc aatagggaac
     2521 tggctaaggt cggcagaacc actcaccccc gccagattca ggcgtacgcc atcaattaac
     2581 accaacacat gactggcatt tgtaccgcga ataaaaatag atgagagctg acctgaaccg
     2641 ccgttttggg tgatatcgac gcccggaaga cggcgcagca catcattgac cgaggtcgac
     2701 tgccagcggt cgatatcctg acgggtcaca acggtggttg gtgcaagcac agtgctgcgc
     2761 ggctgttcaa aacggttagc agtaacgacg agagtatccg ggctggtatc ctgtgcccaa
     2821 gcggaaaagg ctgtgacaga acacgccgtc agcagcgaag cttttttaat cattgtaaag
     2881 catccacaat agaagaagga tgccgcaggt ttcatcaata ttacgcgatg atgagaacca
     2941 gatgcgacgt tggccggcag gtcttcgggc ttggaggggt atctaagata ctaagagatg
     3001 atgacttccc accggatggc agtgtccgca taacgcaatc atcgcacctt tccttaccgc
     3061 tgcgcgtcag ctccagattc gcactggatt ccctattaac tcacaggacc gacaagtgga
     3121 tgctacaggt tgtaacaagt tactgtccag acgtagctca caaataggaa ttcatcaaga
     3181 tctggacatc tgatgagcaa tccctacaat cgccgcgtac tttaattttt caggatacat
     3241 catgaccccc gaacaccttc caacagaaca gtatgaagcg cagttagccg aaaaagtggt
     3301 acgtttgcaa agtatgatgg caccattttc tgacctggtt ccggaagtgt ttcgctcgcc
     3361 ggtcagtcat taccggatgc gtgcggagtt ccgcatctgg cacgatggcg atgacctgta
     3421 tcacatcatt ttcgatcaac aaaccaaaag ccgtatccgc gtggatagct tccccgccgc
     3481 cagtgaactt atcaaccagt tgatgacggc gatgattgcg ggtgtgcgta ataatcccgt
     3541 tctgcgccac aagttgttcc agattgatta cctcaccacg ctgagtaatc aggcggtggt
     3601 ttccctgcta taccataaga agctggatga tgagtggcgt caggaagcgg aggccctgcg
     3661 cgatgcactg cgcgcgcaga atctgaatgt gcatctgatt ggtcgggcaa cgaaaaccaa
     3721 aatcgagctg gatcaagatt acatcgacga gcgtctgccg gtcgcaggga aagagatgat
     3781 ctaccgtcag gtagaaaaca gctttaccca gccgaacgcg gcgatgaata ttcagatgct
     3841 ggaatgggcg ctggacgtaa ccaaaggctc aaaaggcgat ttactggagc tgtactgcgg
     3901 caacggtaac ttttcattag cgctggcgcg caattttgat cgggtattag ccaccgaaat
     3961 cgctaagccg tcggttgctg ctgcgcaata caacatcgca gctaaccata ttgataacgt
     4021 acaaattatt cgtatggcgg cagaagaatt tactcaggcg atgaatggtg tgcgcgagtt
     4081 taaccgcctg caagggatcg acttaaagag ttatcagtgc gaaaccattt ttgtcgaccc
     4141 accgcgcagc ggtctggaca gtgaaaccga gaaaatggtg caggcgtatc cgtgtatttt
     4201 gtacatctcc tgtaacccgg aaacgttatg caagaatctg gaaacattaa gccagacgca
     4261 caaggtcgaa cgtctggctc tgtttgatca gttcccctac acgcaccata tggagtgcgg
     4321 cgtattactg accgcgaagt aaaacccatg ccggatgcgc cagcatccgg cataataccg
     4381 attactcagc aacttcctgc ttacggttac gcatcttcgc gccaatccag aacaccataa
     4441 tgacggagag tactgccggg aagaagttag agccgatatc cggatattcc gcacgaacta
     4501 ccgtgctgta cagcagtacg ccgagaataa aacaggcagc tgccagaccc ggcaagccta
     4561 ccggcatagt gcgattaaga taacgttgat gcaggcagta aaccgtcagc accagggaaa
     4621 taatcgggaa tacagaaaat ggcacaatgg agctaaacag cgctgcgaaa gtaccattaa
     4681 tcgataagcc agcaactaac gccagcagca gcgtacctct atcttgattt gcttgtttca
     4741 ttactcgtcc ttcacatttc ccggaataat tgcggttttc tcttgttcac ggcgatacca
     4801 gtaataagcc cctttcgaaa tcattcgcag ttgcagtacc agtcgctctt ctaattgccg
     4861 acgttgttcg acgccgacgt ccaacgcctc ggcacccgca ctgaagacaa ttgtcaccat
     4921 tgcttcggct tgcgcttcag taaacgcacg cggcatatgg ttttcgagtt ccagatagtc
     4981 cgcaagttcc gcaatgaagt gctgaatttc acgcgcaacg gcggcacgaa acgcagcgga
     5041 ggtgccggag cgttcccgca ataataaccg gaaggcgtta ggattattac cgatgaactc
     5101 cataaatgtg gagaccgagg tgcggatcac actcccgcct ttggcgatac gctgacgcgc
     5161 ctggcgcatg agttggcgta gcattaaacc gctctcatca accatggtca gacccagttc
     5221 gtctacgtcg cggaaatgcc gataaaaaga ggtgggagca atgcccgctt cacgcgccac
     5281 ttcacgcaaa ctcaggctgg cgaagctgcg ttcagcactt aattggctaa atgcggcttc
     5341 caccagcgaa cggcgggttt tttctttttg ttgcgctctt atgcccatca cgatgtctga
     5401 atccttgcca aaagtactgc tggcactata ccagagaatg aacatactgg attgctccag
     5461 tattgtgaat gaacggtaac gcaataaaac atatttactg gccggttctt ttacgtacag
     5521 cggaaacctg ccgcttaaac ggagagtatc gtcgataaaa atccaataaa acgtcagggc
     5581 aaaagtaaga aacagacaaa gcaaaggccg ctcaggatat agccagataa atgacgggga
     5641 tcaattggct tacccgcgat agaatgttac cattctgttg cttttatgta taagaacagg
     5701 taagccctac catgccacat tcctacgatt acgatgccat agtaataggt tccggccccg
     5761 gcggcgaagg cgctgcaatg ggcctggtta agcaaggtgt gcgcgtcgca gttatcgagc
     5821 gttatcaaaa tgttggcggc ggttgcaccc actggggcac catcccgtcg aaagctctcc
     5881 gtcacgccgt cagccgcatt atagaattca atcaaaaccc actatacagc gaccattcgc
     5941 gactgctccg ctcttctttt gccgatatcc ttaaccatgc cgataacgtg attaatcaac
     6001 aaacgcgcat gcgtcaggga ttttacgaac gtaatcactg tgaaatattg caggggaacg
     6061 ctcgctttgt tgacgagcat acgttggcgc tggattgccc ggacggcagc gtcgaaacac
     6121 taaccgctga aaaatttgtt attgcctgcg gctctcgtcc atatcatcca acagatgttg
     6181 atttcaccca tccacgcatt tacgacagcg actcaattct tagcatgcac cacgaaccgc
     6241 gccatgtact tatctatggt gctggagtga tcggctgtga atatgcgtcg atcttccgcg
     6301 gtatggatgt aaaagtggat ctgatcaaca cccgcgatcg cctgctggca tttctcgatc
     6361 aagagatgtc agattctctc tcctatcact tctggaacag tggcgtagtg attcgtcaca
     6421 acgaagagta cgagaagatc gaaggctgtg acgacggtgt gatcatgcat ctgaagtcgg
     6481 gtaaaaaact gaaagctgac tgcctgctct atgccaacgg tcgtaccggt aataccgatt
     6541 cgctggcgtt acagaacatt gggctggaaa ctgacagtcg cggacagctg aaggtcaaca
     6601 gcatgtatca gaccgcacag ccgcacgttt acgcggtggg cgacgtgatt ggttatccga
     6661 gcctggcatc agcggcctat gaccaggggc gcattgccgc gcaggcgctg gtaaaaggcg
     6721 aagccaccgc gcatctgatt gaagatatcc ctaccggcat ttacaccatc ccggaaatca
     6781 gctctgtggg caaaaccgaa cagcagctga ccgcgatgaa agtgccatat gaagtgggcc
     6841 gcgcccagtt taaacatctg gcacgcgcac aaatcgtcgg catgaacgtg ggcacgctga
     6901 aaattttgtt ccatcgggaa acaaaagaga ttctgggtat tcactgcttt ggcgagcgcg
     6961 ctgccgaaat tattcatatc ggtcaggcga ttatggaaca gaaaggtggc ggcaacacta
     7021 ttgagtactt cgtcaacacc acctttaact acccgaccat ggcggaagcc tatcgggtag
     7081 ctgcgttaaa cggtttaaac cgcctgtttt aacactttat cgaaatagcc atccattctt
     7141 gctcggatgg cctctgccag ctgctcatag cggctgcgca gcggtgagcc aggacgataa
     7201 accaggccaa tagtgcggcg tggttccggc ttaatgcacg gcagataaac aaccccatcg
     7261 cgtttgcgct ccggcggcac agccagcgct ggcagtaaag tgatcccgct acctgccgcc
     7321 accatgttgc gcagcgtctc caggctggtc gcgcggaagt gtgtatcttc atccgccccg
     7381 gcttcaaaac agaaacccat tgcctgatcg cgcaaacagt gaccatcttc cagcatcagc
     7441 agtttttccc ctgccagatc ggccatcggt acgcattcgc ggttcgccca cgggtgatct
     7501 tcatagatag ccagcaacat tggctcatca aacaacggca cttcaatgaa tgcttcgctc
     7561 tctttcacca gcgcgaggat cacgcaatcg agtttgccac tgtccagttg cgccagtaac
     7621 tggtgggtct gtgcttcatg cagatacatt tccagctttg gaaaggtctg gtgcagcatc
     7681 gggataatat gcggtagcag gtacggtcca accgtgggaa tcaaaccaat atgcagcggt
     7741 ccggacatcg tctcgccctg ctggcttgcc atctctttaa ggactttcac ctcacgcagc
     7801 acggtacgcg cctgatccac cagcagcatt cccgcctggg tgaacaacac tttacggctg
     7861 gtccgctcca gcaacatcac gcccagctca tcttccagct tacgaatttg cccgctaagc
     7921 gtcggctggc taacgtggca ggaatctgcc gcacgccgaa aatggcggtg ttcagccaat
     7981 gccaccaggt actcaagatc acgaatattc attatccatc ctccatcgcc acgatagttc
     8041 atggcgatag gtagaatagc aatgaacgat tatccctatc aagcattctg actgataatt
     8101 gctcacagaa acggagcggc acctctttta acccttgaag tcactgcccg tttcgagagt
     8161 ttctcaactc gaataactaa agccaacgtg aacttttgcg gatctccagg atccgctttt
     8221 ttttgccata aaaaagcccg gcgataagcc aggctcaaat ttgtacatat aaatgttctt
     8281 accccaaccg cgcctgcgca aaagcaatcg cttgggccac ctgctgcggt gagacgccgc
     8341 cttttgccgc acgcttgtcg aggcacgatt gcagcgacaa aatcggatag acatcttcgc
     8401 caatcaccgg gctgaatttc tgcaactcgt cgagcggcag atcttccagc ggtttgccct
     8461 gacgaatggc ttccaccacc gcttcaccca cgatatggtg cgcctcgcgg aaaggtacgc
     8521 ctttcgccac caggtaatcc gccagttcgg tggcgttggc gtaaccctgc tgagccgctt
     8581 cctggcaacg tggacgtttc acctgaatgc cgtccagcac cagcgccgcc atatgcaggc
     8641 agtccagcca ggtatcgagc gcgtcgaaca gaccttcttt gtcttcctgc atatctttgt
     8701 tgtaagccag cggcaaacct ttcagcgtca tcatcatgcc ggttaacgca ccctgcaccc
     8761 gaccgcattt accgcgaatc agctccagcg catccgggtt tttcttctgc ggcattaatg
     8821 atgaaccgga agtcacgcgg tcagaaagct ccacaaaccc cgcttcgccg gtgttaaaga
     8881 aaatcagatc ttcagcaaaa cgcgacagat gcaccatgcc gatagcggca gccgacagca
     8941 gttccaacac atggtcacgg tcagaaacgc tgtccagact gttacgggtc gccgaagcaa
     9001 agcccagcca gcctgctaac tgttcacggt cgatttcata ggccgttccc gccagcgcgc
     9061 cacagcctaa tgggctgaca tccagacgct taagcgcatc ctgcaaacgg ctttcatcac
     9121 gcgccagcat ctcaacatag gccaggcacc agtgcgcgaa cgtcaccggc tgggcgcgtt
     9181 gcaggtgagt gtaacctggc atgaccgcgt cctgattgtt ttgtgcggtt tccaccagcg
     9241 ccgattgcag ctgtcggtta gccgtcagta actcgctaac ggtatctttg caccacagtt
     9301 tcaggtcagt cgctacctga tcgttacgac tacgcccggt atgcagcttt ttgcctaact
     9361 ggcccacttt gtcgatcagt ttgccttcca cccagctatg gatatcttcg gcgtcgcttt
     9421 caaggatttg ttgtggcctg gcgcgaacat cttccagcag cacgttcagc gcctcttcca
     9481 gttgcgcctg ctcttctgcg gttaacacgc ctaccgtgac cagggctttg gaccaggcca
     9541 cagagccaac aatatcctgc tccgccagac ggtaatcaaa gcgcagtgag tcgttgaatt
     9601 gtttgaaccg ttgatctgct gcctgggtaa aacgcccgcc ccaaagtgcc ataactctgt
     9661 ttccttattt tgaaattcaa tgccggaaag ctgcgcctcc ggccaacaaa acttaagcta
     9721 aaatccgcgt acccatcggc ataccgttaa acagtgccgg aagctgctcc gcatgacgcc
     9781 aggaggcgat atctaccgga cggcccagcg tgcgggccgc atccagcgcc gcgttcactt
     9841 tcactatcat gccgtcagta ataatgccct gctcaatcag ttgttctgct ttcgcggcgg
     9901 tcatttcggc aatgcgttgc cctttgccgt cgagaatgcc gctgacgtcg gagagcaaaa
     9961 tcagatccgc gcccagcgtt gccgccagcg ccgttgccgc ctggtcggca ttgacgttca
    10021 tcagttgccc ttcgtctgtt acgccaatgg agctgaccac cggcagataa ccgttctcca
    10081 gcaaggagtt gataagctta ggcgaacctg gctgcgccag tccaacatga cctaactctt
    10141 catcaagctg ggtcactttg acgctgtcgc cgtcaccgag aaacaaacct acggccgcaa
    10201 tctgatgttt cttcgcccat gccaacaggg ttttatttgc cgttcccgcc agtgctccgg
    10261 tgataatgtc tatctgatca gcaggcgtca cccgcaggcc gtttttcttt ttcaccggca
    10321 gattcagccc tttcatcagc tcatccacca cgcaaccgcc gccgtgcaca atcaccagcg
    10381 gacgctgatg tgactcacga taattcacca gtgtgctaaa cagacgttcc agcgcctctt
    10441 cactatccag cagtacgccg cccagtttga taattaatgg attcatcatt gcacccttaa
    10501 ataagagact gcgtttccgc atagccgaaa cgaatattgg cgcactgtac cgcttgtgcc
    10561 gccgcgcctt tcagtaagtt gtcttcggtc gccacaataa tcagatgctc gccctgaacg
    10621 gcaaacccga tatcgcaaaa tggcagccca acgacatttt tcagcgccgg aacgcctttg
    10681 tcatacagcc gcaccagcgg tttatgggca tacgcctgct gtaacacttg cgcgacttgc
    10741 gcctgggtca cacccgattt caggcggcag gtaatggttt cgagaatgcc gcgcgggaaa
    10801 ttgcccagat gtggggtgaa gataacgtca gcaccgaggt gtgtggcgat ctctggttga
    10861 tggcgatgag taaagacgcc atacggttgc aggctaactt cacaaaaact atttgaaatc
    10921 gctgccttac gtcctgcacc gctcacgccg ctggtggcgt tgatcaccgg ccactgattg
    10981 aggtcaagaa gatcggcatc aatcaacggt ttcagcgcca gctgtgccgc cgtcggataa
    11041 cagcctggca ccgcaatcaa attcgcttct tttaatttat taccgcacca ctccgccaga
    11101 ccgtaggctg cctgttccag cagttccggg tattgatggg taaagccgta atatttttca
    11161 tagaaggtgg cgtcgttaac acgaaacgca ccggaaaggt cgaacaccac gcagcccgct
    11221 tcaagaaact gcggcgctaa atcgtggcta acttcatggg cggtggcgag aaacactacg
    11281 tccacccctg ggctaaactc gctgatatcc gacatcggct gcaacggcag ctcaacgatg
    11341 ccttttagct gcggatgcaa atcggagatt aactttcccg catcattgct ttgcgctgaa
    11401 acagtcaaag cggttatgtt catatgcgga tggcgattta cataggtcac tagctctgcg
    11461 ccagcgtagc cgctggcacc cacaatcagc gtattcaaca tcggggctat tcaccttctt
    11521 atgtctggtt gccaggttaa acgtaaaaca ttcaccttac ggctggtggg ttttattacg
    11581 ctcaacgtta gtgtattttt attcataaat actgcatgaa tattgatact atcatgacca
    11641 gaggtgtgtc aacaatgaaa aacaaattac cgccatttat cgagatttac cgtgctctga
    11701 ttgccacacc ttcaataagc gccacggaag aggcactcga tcaaagcaat gcagatttaa
    11761 tcactctgct ggcggactgg tttaaagatt taggcttcaa tgtggaagtg cagcctgtgc
    11821 caggaactcg caacaaattc aatatgctgg caagtatcgg acagggggct ggcggcttgt
    11881 tgctggcggg gcataccgat acggtgccat ttgatgacgg tcgctggacg cgcgatccgt
    11941 ttacactgac ggagcatgac ggcaagcttt acggcttagg caccgccgac atgaaaggct
    12001 tttttgcgtt tatccttgat acgctacgcg atgtcgacgt cacgaaactg aaaaaaccgc
    12061 tctacattct ggcgactgct gatgaagaaa ccagtatggc cggagcgcgt tattttgccg
    12121 aaactaccgc cctgcgcccg gattgcgcca tcattggcga accgacgtca ctacaaccgg
    12181 tacgcgcaca taaaggtcat atctctaacg ccatccgtat tcagggccag tcggggcact
    12241 ccagcgatcc agcacgcgga gttaacgcta tcgaactaat gcacgacgcc atcgggcata
    12301 ttttgcaatt gcgcgataac ctgaaagaac gttatcacta cgaagcattt accgtgccat
    12361 accctacgct caacctcggg catattcacg gtggcgacgc ttctaaccgt atttgcgctt
    12421 gctgtgagtt gcatatggat attcgtccgc tgcctggcat gacactcaat gaacttaatg
    12481 gtttgctcaa cgatgcattg gctccggtga gcgaacgctg gccgggtcgt ctgacggtcg
    12541 acgagctgca tccgccgatc cctggctatg aatgcccacc gaatcatcaa ctggttgaag
    12601 tggttgagaa attgctcgga gcaaaaaccg aagtggtgaa ttactgtacc gaagcgccgt
    12661 ttattcaaac gttatgcccg acgctggtgc tcggtccggg ttccatcaat caagctcatc
    12721 agcctgatga atatctggag acgcggttta ttaaacccac ccgtgagttg attatccagg
    12781 taattcacca tttttgctgg cattaaaacg taggccggat aaggcgctcg cgccgcatcc
    12841 ggcaccgttg ccaaactccg gtgccgcaat aatgtcggat gcgatgcttg cgcatcttat
    12901 ccgacctaca cctttggtgt tatgtatggc gattttttaa catttccata agttacgctt
    12961 atttaaagcg tcgtgaattt aatggcgtaa attcctgcta tttattcgtt tgctgaagcg
    13021 atttcgcagc atttgacgtc accgctttta cgtggcttta taaaagacga cgaaaagcaa
    13081 agcccgagca tattcgcgcc aatgcgacgt gaaggataca gggctatcaa acgataagat
    13141 ggggtgtctg gggtaatatg aacgaacaat attccgcatt gcgtagtaat gtcagtatgc
    13201 tcggcaaagt gctgggagaa accatcaagg atgcgttggg agaacacatt cttgaacgcg
    13261 tagaaactat ccgtaagttg tcgaaatctt cacgcgctgg caatgatgct aaccgccagg
    13321 agttgctcac caccttacaa aatttgtcga acgacgagct gctgcccgtt gcgcgtgcgt
    13381 ttagtcagtt cctgaaccta gccaacaccg ccgagcaata ccacagcatt tcgccgaaag
    13441 gcgaagctgc cagcaacccg gaagtgatcg cccgcaccct gcgcaaactg aaaaaccagc
    13501 cggaactgag cgaagacacc atcaaaaaag cagtggaatc gctgtcgctg gaactggtcc
    13561 tcacggctca cccaaccgaa attacccgtc gtacactgat ccacaaaatg gtggaagtga
    13621 acgcctgctt aaaacagctc gataacaaag atatcgctga ctacgaacac aaccagctga
    13681 tgcgtcgcct gcgccagtta atcgcccagt catggcatac tgatgaaatc cgtaagctgc
    13741 gtccaagccc ggtagatgaa gccaaatggg gctttgccgt agtagaaaac agcctgtggc
    13801 aaggcgtacc gaattacttg cgcgaactga acgaacaact ggaagagaac ctcggctaca
    13861 aactgcccgt cgaatttgtt ccggtccgtt ttacttcgtg gatgggcggt gaccgcgacg
    13921 gcaacccgaa cgtcactgcc gatatcaccc gccacgtcct gctactcagt cgctggaaag
    13981 ccaccgatct gttcctgaaa gatattcagg ttctggtttc tgaactgtcg atggttgaag
    14041 cgacccctga actgctggcg ctggttggcg aagaaggtgc cgcagaaccg tatcgctatc
    14101 tgatgaaaaa cctgcgttct cgcctgatgg cgacacaggc atggctggaa gcgcgcctga
    14161 aaggcgaaga actgccaaaa ccagaagggc tgctgacaca aaacgaagaa ctgtgggaac
    14221 cgctctacgc ttgctaccag tcacttcagg cgtgtggcat gggtattatc gccaacggcg
    14281 atctgctcga caccctgcgc cgcgtgaaat gtttcggcgt accgctggtc cgtattgata
    14341 tccgtcagga gagcacccgc cataccgaag cgctgggcga gctgacccgc tacctcggta
    14401 tcggcgacta cgaaagctgg tcagaggccg acaaacaggc gttcctgatc cgcgaactga
    14461 actccaaacg tccgcttctg ccgcgcaact ggcaaccaag cgccgaaacg cgcgaagtgc
    14521 tcgatacctg ccaggtgatt gccgaagcgc cgcaaggctc cattgccgcc tacgtgatct
    14581 cgatggcgaa aacgccgtcc gacgtactgg ctgtccacct gctgctgaaa gaagcgggta
    14641 tcgggtttgc gatgccggtt gctccgctgt ttgaaaccct cgatgacctg aacaacgcca
    14701 acgatgtcat gacccagctg ctcaatatcg actggtatcg cggcctgatt cagggcaaac
    14761 agatggtgat gattggctat tccgactcag caaaagatgc gggcgtgatg gcagcttcct
    14821 gggcgcaata tcaggcacag gatgcattaa tcaaaacctg cgaaaaagcg ggtattgagc
    14881 tgacgttgtt ccacggtcgc ggcggttcca ttggtcgcgg cggcgcacct gcccatgcgg
    14941 cgctgctgtc acaaccgcca ggaagcctga aaggtggcct gcgcgtgact gaacagggcg
    15001 agatgatccg ctttaaatat ggtctgccag aaatcaccgt cagcagcctg tcgctttaca
    15061 ccggggcgat tctggaagcc aacctgctgc caccacctga gccgaaagag agctggcgtc
    15121 gcattatgga tgaactgtca gtcatctcct gcgatctcta ccgtggctac gtacgtgaaa
    15181 acaaagattt tgtgccttac ttccgctccg ctacgccgga acaagaattg ggcaaactgc
    15241 cgttgggttc acgtccggcg aaacgtcgcc caaccggcgg cgtcgagtcg ctgcgcgcca
    15301 taccgtggat ttttgcctgg acgcaaaacc gcctgatgct ccccgcctgg ctgggtgcag
    15361 gtacggcgct gcaaaaagtg gtcgaagatg gcaaacagag cgagctggaa gccatgtgcc
    15421 gcgattggcc attcttctcg acgcgtctcg gcatgctgga gatggtcttc gccaaagcag
    15481 acctgtggtt ggcggaatac tatgatcaac gtctggtaga caaagcactg tggccgttag
    15541 gtaaagagtt acgcaacctg caagaagaag acatcaaagt ggtgctggcg attgccaacg
    15601 attcccatct gatggccgat ctgccgtgga ttgcagagtc tattcagcta cggaatattt
    15661 acaccgaccc gctgaacgta ttgcaggccg agttgctgca ccgctcccgc caggcagaaa
    15721 aagaaggcca ggaaccggat cctcgcgtcg aacaagcgtt aatggtcact attgccggga
    15781 ttgcggcagg tatgcgtaat accggctaat cttcctcttc tgcaaaccct cgtgcttttg
    15841 cgcgagggtt ttctgaaata cttctgttct aacaccctcg ttttcaatat atttctgtct
    15901 gcattttatt caaattctga atataccttc agatatcctt aaggaattgt cgttacattc
    15961 ggcgatattt ttttcaagac aggttcttac tatgcattcc acagaagtcc aggctaaacc
    16021 cctttttagc tggaaagccc tgggttgggc actgctctac ttttggtttt tctctactct
    16081 gctacaggcc attatttaca tcagtggtta tagtggcacc aacggcattc gcgactcgct
    16141 gttattcagc tcgctgtggt tgatcccggt attcctcttt ccgaagcgga ttaaaattat
    16201 tgccgcagta atcggcgtgg tgctatgggc ggcctctctg gcggcgctat gctactacgt
    16261 catctacggg caggagttct cgcagagcgt tctgtttgtg atgttcgaaa ccaacaccaa
    16321 cgaagccagc gagtatttaa gccagtattt cagcctgaaa attgtgctta tcgcgctggc
    16381 ctatacggcg gtggcagttc tgctgtggac acgcctgcgc ccggtctata ttccaaagcc
    16441 gtggcgttat gttgtctctt ttgccctgct ttatggcttg attctgcatc cgatcgccat
    16501 gaatacgttt atcaaaaaca agccgtttga gaaaacgttg gataacctgg cctcgcgtat
    16561 ggagcctgcc gcaccgtggc aattcctgac cggctattat cagtatcgtc agcaactaaa
    16621 ctcgctaaca aagttactga atgaaaataa tgccttgccg ccactggcta atttcaaaga
    16681 tgaatcgggt aacgaaccgc gcactttagt gctggtgatt ggcgagtcga cccagcgcgg
    16741 acgcatgagt ctgtacggtt atccgcgtga aaccacgccg gagctggatg cgctgcataa
    16801 aaccgatccg aatctgaccg tgtttaataa cgtagttacg tctcgtccgt acaccattga
    16861 aatcctgcaa caggcgctga cctttgccaa tgaaaagaac ccggatctgt atctgacgca
    16921 gccgtcgctg atgaacatga tgaaacaggc gggttataaa accttctgga tcaccaacca
    16981 gcagacgatg accgcccgca ataccatgct gacggtattt tcgcgccaga ccgacaagca
    17041 gtactacatg aaccagcaac gtacgcagag tgcgcgtgaa tacgacacca acgtgctgaa
    17101 gccgttccag gatgtgctga atgaccctgc gccgaagaaa ctgatcatcg ttcatctgct
    17161 gggtacgcat atcaaataca aataccgcta cccggaaaat cagggcaagt ttgatggcaa
    17221 taccgatcat gttccgccag gattaagcgc agaagagctg gaatcatata acgattatga
    17281 caacgctaac ctgtataacg atcatgtggt tgccagcctg attaaagact ttaaagcggc
    17341 agacccgaac ggattcctgg tttacttctc tgaccacggt gaagaggttt acgacacgcc
    17401 gccgcacaaa actcaggggc gtaacgaaga caacccgacg cgccacatgt acaccattcc
    17461 gttcctgctg tggacgtcgg aaaaatggca agcgactcat ccccgtgatt tctcacagga
    17521 tgtcgatcgt aaatacagcc tggcggaact gatccacacc tggtcagatt tggcgggctt
    17581 atcttacgac ggttacgatc caacccgttc agtggtgaat ccgcagttca aagaaactac
    17641 ccgctggatt ggtaacccgt acaagaaaaa cgcgctgatc gattacgaca ctctgccgta
    17701 tggcgaccag gtaggtaatc agtaattatc agccaggcgc tatttgccgg atgcgacgct
    17761 tgacgcgtct tatccggcct acagatcgcg gcggtaggct ggataaggcg tttacgccac
    17821 atccggcaat acaactgccg caatgtttac atcgaactcc cctcaggcca cacttttcag
    17881 gtggcctttt ttaataatgc cagttactgt tttcccgtct acatacctaa gggaataaca
    17941 tgtatcacga cgtcagctat ctgctctccc gcctgatcaa cggcccgctg tccctgcgcc
    18001 agatttactt tgccagcagt aacggcccgg tgcccgatct tgcgtatcag gtcgattttc
    18061 cacggctgga aattgtgctg gaaggtgagt ttgttgatac cggcgctgga gcaacgttag
    18121 ttcccggcga tgtgctgtac gttgccgctg gtggctggaa tttcccacaa tggaaaacac
    18181 ccgctaccac ctttagcgtg ctgtttggca aacagcaact cggcttcagc gtcgtgcaat
    18241 gggatggcaa acaatatcaa aatctggcga agcaacacgt cgcccgacgc ggcccacgta
    18301 taggttcttt tctgctacaa acgctcaatg aaatgcaaat gcagccgcag gagcagcaaa
    18361 cggcaaggct tatcgtagcc agcctgctta gccactgccg cgatttgctc ggcagccaaa
    18421 tccagaccgc ctcacgcagc caggcactat ttgaagctat tcgcgattat atcgacgaac
    18481 gctatgcctc cgcgcttacc cgcgaatctg tcgcacaggc attctatatt tcaccaaact
    18541 atctctcgca cctgtttcaa aaaacggggg ccattggttt taacgaatac ctgaatcaca
    18601 cgcgactgga gcacgctaag acgttattga aaggctatga cctgaaggta aaagaagtgg
    18661 cgcacgcctg cggctttgtc gacagcaact acttctgccg tctgtttcgt aaaaacaccg
    18721 aacgctcgcc gtcggaatat cgccggcagt accacagcca actgacagaa aaaccgacta
    18781 ctccagaata agatgggttt gctgcggcgc agaaagcact ttgcgcaccg cgctcattac
    18841 ccgctgcggc tcacgcagga atgcgtagat gctgcattgc acatagcggc aatgttcaaa
    18901 tcgctcggca ccggcaagct cgatatccgt aatcaacaga gcaacatccg cccgacggat
    18961 atcctcgtcc gctaaacgat tctccgttcc cagcgcgccc tgagtttcaa tgctgacgcc
    19021 ccacttctct aactggcaca acttttccag ccgttccgcc gccatataag tatgcgccac
    19081 gccgcttacg caggcggtta ctgccaccag gtatgccatt ttaacctccc acggtaacct
    19141 gaaatccggc ccgttctgcc atttcgcgca tcgttgtcac atcggctgac gacggcgcag
    19201 gcacctcttt catcgaccat gttttcccca gcaggcggta tttcggttcg ccgtactgat
    19261 gaaacggtaa cagatggatc tgcctgattt tcagcgggat cagtacatcc agcgcctgct
    19321 gcatattctc cctgctgagc gtgaaaccag ggatcaacgg taaacgcggg atcacgttga
    19381 caccctcact caccagcaaa cgcagattct ccagcacgcg tggcaggttc atcttcacca
    19441 catcccgcgc ctgagtcgcg tccataattt ttaaatcgaa cagcacttca tcgcacaatt
    19501 tcgccagcgg taacagcttg cttgctggcg cgtctccggc agtttcaatg gcgcatgaca
    19561 caccccacag ccgcagtcgc tgtaaaaaac gggtagcaaa ctccgcctgc attaacactt
    19621 cgccgccaga aagcgtgacg ccgccgccgg acgtgcgaaa gaaaatgtca tctttcatca
    19681 cttcccgttc cagagcgtca aggctgatat cgcgaccaat ccgttcaaac gccccggagg
    19741 ggcattcatc cgcatcacgc aaacatttcg cacagtgcag acatttcgcc tctctgcgta
    19801 ccgtctggat tttgccggag atcgactccg gattagcaca ccacgggcaa agatgcggac
    19861 agcctttaaa aaagaccacc gtacgaatgc cctcgccgtc attcagtgaa taacgctgaa
    19921 tgttgaaaat gcgcgccaca tcatcgcggc gcgtttccac aacgttacag ctgatgcgct
    19981 gtccggcgga tgatgtcatc ctggatctcc ttcgacagtt cgacaaagaa ggcgctgtat
    20041 ccggcaacgc gcaccaccag cccggcataa tcttgtgggc gctgttgcgc ttcccgcaac
    20101 gtgtcggcgt tcaccacgtt aaactgaata tgctgcaact taagctgggt aaacgcccgt
    20161 aagaagtcgg ccagtttgcg taatcccgct tcaccttcca gggtcgccgg agtgaatttc
    20221 acgttcagca acgtaccgtt agacagcagc gtattatcga gcttactgac tgacttcagt
    20281 accgctgttg gcccttgtgc gtcctggccc agcataggtg acaagccgcc gtctgccagt
    20341 tgttccccgg caaaacgacc gtctggcgtc gcgccaacca ccgatcccaa cgggacgtga
    20401 gcagaaacgg tatacgatcc cggcgtgaag taaccgcgcg cgggttctgg tatttttcca
    20461 cttctttgca gtagtggcgt aacagttcgg cgctgatgtt atccacctcg tcgatatcgt
    20521 taccgtattt ctcgaagcgg ttaattaagc gagcacggac tttttcgcct tctggcgttg
    20581 caaagttggc ttttaatacc gacagcaatt cgtcaaaact taaacgctgt tgctcaaaaa
    20641 ccatcccttt caacgcatgg agagaatcgc tcaggttggc gataccgatc ccctgtacgc
    20701 cggagaagtt ataacgcgcg ccgccatcgg taatatcgcg acctttttcc agacaatcgc
    20761 tgataaaaga cgagagcagc ggtacaggtg cccagtcgcg atggccgata tcacaaatat
    20821 tgctgccctc aaccatcagg gtgatgtagt ggctgatctt ggcgcggatc tgctccagta
    20881 aaccttcata agtcagcgca gcattgcctt cattttcatg caggcagatt tccatcactt
    20941 tcagcagatt aaacatcgcg atgtcatgca agccgtaggt tctgccggga atagataatt
    21001 ccacacagcc cactacggaa tagtcgcgcg catcttccag cgaaacgcca cggttgagga
    21061 acgctggtac caccacttca tcgttaaaga tttgcggaat accggtgccg aggcgaatgg
    21121 tttcggcggt tttcatcagg aacggcgtgt cgatcaaggc gttagtgcgc acgccgaggt
    21181 tcggttgcgg taattgcacg ctttgatagg cgtcaaggca aaggaacgaa agcacgttca
    21241 ccgcgctacg tccgttctcg gttaacccgc cgagcagtgc ggtatagccg gtcgggaaac
    21301 ctgcgaaata acgcgcgctg ctggtggagc gcaacagcac gatgtcgttg catttcaccc
    21361 ataaagattc gagcagttct ttcaggaacg ccggatcttc gccctgggtt aaagatgcct
    21421 gatagaacgg caacatatac tggtcgaagc gccctaaaga cagcgaactg gcgttggatt
    21481 cgtattgcag aatgatgttc atgtaccaga ataactggca cgcctgccag aacgtctgcg
    21541 gcttatgttc ggcgttatgg cgagagatct ccgcaatagt cagcagttct tcgcgacgct
    21601 gtgcatctgt gcagtttgcc gccatcgttt ccgccagttc ggcgtaacgc aaaatgtgtt
    21661 tctgcgaggc ttccagcagt aacaacgcgg cctgataaaa gtgattctcc ggctgttgct
    21721 gacaatgttg ctgcatctgt gccaccagct cacccagacc gtgattcaac aggcgtgggt
    21781 aatcaataat aatgtgcccc tggcctttat ccgtctggtt gatgctgaaa atctgcgtgt
    21841 tggtcgcggc ttttacttca tctgtcatct gcccgttgat gaaatctttc atcgaacgtt
    21901 tttcccagta cgggaacaac tcttcacggt agatacgttt atcttcttcg ctgatagcaa
    21961 agcggtcctg cggacgcgtc gggaattgat ccagctcttt cagcagccag taagggtcca
    22021 tttccggcga cataatcccg gcgcgcggtt ttacggtgcg gttaccggca atcagttctt
    22081 catcacgaat cgaaatttca acatgttcaa ggatatacgc tgttgctttc gcccggcgca
    22141 atatcaccgg ttcgccttcg gtttgccgat ggctggcggt ataaagcagc gcccgctcca
    22201 gcgagatttc acgggtattg gcaaacagtg cagttttgag gcgagagata cgattcgtca
    22261 tgaggaactc ctgtgggaaa gcgaacccgc ccttgcgagc gggagaagaa cttaagcggt
    22321 ttgcgccagg tgagcttcaa ttttgctcat aatggcgtca gcacgtttta ctgcatcgct
    22381 gatgttgacg cgcacgatgg tcttgccagc aaaacgctct tcaaacttga tgccgatatc
    22441 tttggtcaga atcaccatat cagcgctcgc cacatcttcc gcagtcagtt cattttccag
    22501 accaattgac ccctaggttt ctactttcac ttcccagcct ttcgctttcg cggcactttc
    22561 cagcgcctct gctgccatat aggtatggac aacacctgaa ggacatgcgg ttactgcaat
    22621 aattttcgtc atggctcgtt cctcaattaa ttaatttcaa aatccaaatc caggtcgtct
    22681 tctttttcat cagtcgaaga cccattttta cgcgccagac ttttcagcac gttcacacaa
    22741 acagccgtca ccactgctcc gacagccact gctgcgatat aacccagctt gccttcaacc
    22801 accggcagca caatcagacc accccagcct gcatagcact gcgcaccgac cagcgccgca
    22861 gttactgcac cacaaactga accgaccatg atggaaggaa tgacacgcag cggatcggca
    22921 gcggcgaaag gaatcgcccc ttcagtaacg ccaacacagc ccatcaccag tgccgctttg
    22981 ccggtttcgc gctcttctgc ggagaaattt ttacgaccaa tcagcgtcgc cagccccatc
    23041 cccagcggcg ggatacaaat acccacggca gcgatggcca ccacggtata aacaccctga
    23101 gcaacgcaaa tcagcatgaa ggcataggcc actttgttaa ccggaccgcc catatcgaac
    23161 gccagcatca gacccatgat caccgccagc ataacaatgc tgccctgctg catcccctga
    23221 agccactgag tcaggctgtt ggtcaacgcc cctacaggct cgcccaagcc ccacatcatg
    23281 atgcctgcgg taatcagtgt gccgacgata ggaataatga agataggcat caccgagcgc
    23341 agaactttat gcaccggaat tttcttcagg taatgcacca cgatgccgcc gataatcccg
    23401 gcgatcagtg caccaaagaa gcccgcacca aagctgttac caacccaggt accgatagcg
    23461 catggagcca gcgcagaacg ttctgcaatg gaataaccaa tgtaagcggc gaggaaaggc
    23521 accatcagcg tcaagcccgc aacgccgata tcaaacagtt ttttcagatt tggatcggct
    23581 acggcatccg gcactgcgcc tttgccatac aacatgacgg aaaccgccag caaaataccg
    23641 cccgatacca cgaagggaat catgtgtgaa acgcccgtca ttaaatgctg acgggtattt
    23701 ttcaggatct gcaccaactc attcataagc tgctcccggg cttgcgaaag cccatgtcca
    23761 tatttttgat ggaggaaaca ataggcaatc gcaggcggag caaacagtgg ataaaaaggc
    23821 catttactgg acttttgtaa tatcagtaca aaaatgcgat ccgcctcata acttgcgata
    23881 aaccggaagc atgccgcaaa acggcgtctt gtgagacgcg tcgcaaattg acggttacat
    23941 tacatttgtc cagcttttgg cagatttgtc acatggcagg ccgttgccgt gcgctcttat
    24001 cctgtaaaca gaaaaccgta ccgggagaga ggcaatggcc ctgattgtgg aatttatttg
    24061 tgagctacct aacggcgtac atgcgcgtcc ggcaagccac gttgaaacgc tgtgtaatac
    24121 tttttcatca caaattgagt ggcataacct gcacactgac cgcaagggca acgccaaaag
    24181 cgcccttgcg ctgattggca ccgatacgct ggcgggcgat aactgccagt tactgatttc
    24241 cggggccgac gaacaggaag cgcaccagcg tttaagccaa tggctgcgcg atgaattccc
    24301 ccactgcgat gcgccgctgg cggaagttaa atctgacgaa ctggaaccac tgccggtttc
    24361 actgaccaat ctgaatccgc aaattatccg cgcccgcacc gtgtgcagcg gtagtgcagg
    24421 cggcattctg acgccgatct cttctttaga tctcaatgcg ctgagtaatc ttcccgcagc
    24481 caaagacgtt gacgccgagc aatccacact ggaaaacggc ctgacgctgg tactgaaaaa
    24541 cattgagttt cgtctgctgg atagcgacgg tgctaccagc gcgatactgg aagctcaccg
    24601 atccctggct ggcgatacat ctttacgtca gcacttactg gcaggcgtca gcgagggatt
    24661 aagctgcgcc gaagcgattg tcgccagtgc gaatcacttt tgcgaagagt tcgcccgttc
    24721 cagcagcagc tacctgcaag aacgtgccct ggacgtacgc gacgtctgct tccagttact
    24781 ccagcaaatc tacggtgagc aacgcttccc ggcaccgggc aaactgacgc agtccgccat
    24841 ttgtatggct gatgaactga cccccagcca gttcctcgaa ctggataaaa atcacctcaa
    24901 aggtctgttg ctgaaaagtg gcggcaccac ctcacatacg gtgatccttg cccgttcgtt
    24961 caacattccg acgctggttg gtgtggatat tgatgccctc actccgtggc agcatcaaac
    25021 gatttatatc gacggcaacg ccggggcgat tgtggttgag ccaggagaag ccgtagctcg
    25081 ttattatcag caagaagccc gcgtacagga cgccctgcgt gagcaacagc gtgtctggct
    25141 gacccaacaa gcccgtaccg ctgacggtat ccgcattgaa attgccgcta acatcgctca
    25201 ctccgtggaa gcgcaggccg cattcggcaa tggtgcggaa ggcgttggtt tgttccgcac
    25261 tgaaatgctc tatatggatc gcaccagtgc accgggcgaa agcgagctgt acaacatttt
    25321 ttgtcaggcg ctggagtctg ccaacggacg cagcattatt gtgcgcacta tggacattgg
    25381 cggcgacaaa cccgttgatt atctaaacat tcccgcagag gcaaacccgt tcctcggtta
    25441 tcgcgccgtg cgtatttatg aagaatacgc gtcgttattt accacacaac tacgctcgat
    25501 cctgcgcgcc tccgctcacg gcagcctgaa aatcatgatc ccgatgatct cctcaatgga
    25561 agagatctta tgggtgaaag aaaaactggc agaagccaaa cagcaactac gtaacgaaca
    25621 cattccgttt gatgagaaga tccagctcgg cattatgctg gaagtgccgt cggtgatgtt
    25681 catcatcgat caatgctgcg aagagattga tttctttagt attggtagta atgacctgac
    25741 gcaatatctg ctggcagtgg atcgcgataa cgctaaggtt actcgtcact acaacagcct
    25801 gaatccggca ttcttgcggg cgctcgatta cgccgtgcag gcggtgcatc gccagggtaa
    25861 atggattggt ctgtgcggtg agctgggagc gaaaggttcc gtgctgccgt tgctggtcgg
    25921 cttagggctg gatgaactca gcatgagcgc accatcaatt ccggcggcga aagcgcggat
    25981 ggcgcaactt gatagccgtg agtgccgcaa gttgctcaac caggcaatgg cctgccgtac
    26041 ttcgctggaa gtggagcacc tgctggcgca attccgcatg acccaacaag acgcaccgct
    26101 ggtcaccgcc gagtgcatca cactggaaag cgactggcgc agcaaagaag aagtgctcaa
    26161 aggcatgacc gataacctgc tgctggcagg gcgctgccgc tatccgcgta aactggaagc
    26221 cgacttgtgg gcgcgcgagg ccgttttctc taccggtctg ggctttagtt ttgccattcc
    26281 acacagcaaa tcagaacaca ttgagcaatc caccatcagc gttgcgcgtc tgcaagcgcc
    26341 ggtgcgctgg ggtgatgatg aagcgcaatt catcatcatg ttaaccctga acaaacacgc
    26401 tgcgggcgat cagcacatgc gcattttctc gcgcctcgct cgccgcatca tgcacgaaga
    26461 attccgtaac gcgctggtta acgccgcctc tgccgacgct atcgccagcc tgctgcaaca
    26521 tgaactggaa ctgtaaaagg aaacatcatg gaactgtatc tggacaccgc taacgtcgca
    26581 gaagtcgaac gtctggcacg catattcccc attgccgggg tgacaactaa cccgagcatt
    26641 atcgctgcca gcaaggagtc catatgggaa gtgctgccgc gtctgcaaaa agcgattggt
    26701 gatgagggca ttctgtttgc tcagaccatg agccgcgacg cgcaggggat ggtggaagaa
    26761 gcgaagcgcc tgcgcgacgc tattccgggt attgtggtga aaatcccggt gacttccgaa
    26821 ggtctggcag caattaaaat actgaaaaaa gagggtatta ctacgcttgg caccgcagtg
    26881 tacagtgctg cacaagggtt actggccgcg ctggcagggg caaaatacgt tgctccgtat
    26941 gttaaccgcg tagatgccca gggcggagac ggcattcgta cggttcagga gctgcaagcg
    27001 ctgttagaaa tgcacgcgcc agaaagcatg gtgctggcag ccagctttaa aacgccgcgt
    27061 caggcgctgg actgtttact ggcaggatgt gaatccatca ccctgccctt agatgtagcg
    27121 caacaaatgc tcaacacccc tgcggtagag tcagctatag agaagttcga acacgactgg
    27181 aatgccgcat ttggcactac tcatctctaa aggagcaatt atggaccgca ttattcaatc
    27241 accgggtaaa tacatccagg gcgctgatgt gattaatcgt ctgggcgaat acctgaagcc
    27301 gctggcagaa cgctggttag tggtgggtga caaatttgtt ttaggttttg ctcaatccac
    27361 tgtcgagaaa agctttaaag atgctggact ggtagtagaa attgcgccgt ttggcggtgg
    27421 atgttcgcaa aatgagatcg accgtctgcg tggcatcgcg gagactgcgc agtgtggcgc
    27481 aattctcggt atcggtggcg gaaaaaccct cgatacagcc aaagcactgg cacatttcat
    27541 gggtgttccg gtagcgatcg caccgactat cgcctctacc gatgcaccgt gcagcgcatt
    27601 gtctgttatc tacaccgatg agggtgagtt tgaccgctat ctgctgttgc caaataaccc
    27661 gaatatggtc attgtcgaca ccaaaatcgt cgctggcgca cctgcacgtc tgttagcggc
    27721 gggtatcggc gatgcgctgg caacctggtt tgaagcgcgt gcctgctctc gtagcggcgc
    27781 gaccaccatg gcgggcggca agtgcaccca ggctgcgctg gcactggctg aactgtgcta
    27841 caacaccctg ctggaagaag gcgaaaaagc gatgcttgct gccgaacagc atgtagtgac
    27901 tccggcgctg gagcgcgtga ttgaagcgaa cacctatttg agcggtgttg gttttgaaag
    27961 tggtggtctg gctgcggcgc acgcagtgca taacggcctg accgctatcc cggacgcgca
    28021 tcactattat cacggtgaaa aagtggcatt tggtacgctg acgcagctgg ttctggaaaa
    28081 cgcaccggtt gaggaaatcg aaaccgtagc tgcacttagc catgcggtag gtttgccaat
    28141 aactctcgct caactggata ttaaagaaga tgtcccggcg aaaatgcgaa ttgtggctga
    28201 agcggcatgt gcagaatgtg aaaccatcca caacatgcct ggcggcgcga cgccagatca
    28261 ggtttacgcc gctctgctgg tagccgacca gtacggtcag cgtttcctgc aagagtggga
    28321 ataacctact ccaaactccc ggcttgtccg ggagtttgaa cgcaaaattg cctgatgcgc
    28381 tacgcttatc aggcctacgc aatctctgca atatattgaa ttttcttgtt ttttaggaca
    28441 gataaggcgt tcacgccgca tccggcactc tgtgccagta atctgaaact cccacgttgt
    28501 tcaggagttt tttatccgct tctggacatt ttctctacag aacaatcgtt atcccggaag
    28561 gtcgaatctg tgactcttat tttcacggac gaaccagatg aaagcttcac tggcgctgct
    28621 cagcctgcta acggcattta ccagccactc cttaaaatct cccgccgttc caccaaccgt
    28681 ggtacaaatc caggccaata ccaaccttgc catcgcagac ggtgccagac agcaaatcgg
    28741 cagcacgcta ttttacgatc cggcgtatat gcagcttacc tatcctggcg gtgatgtccc
    28801 gcaaaaacgc ggggtatgtt ccgatgtggt gatccgtgca ttgcgcagcc agaaggtcga
    28861 tttgcagaaa ctggtgcacg aagatatggc gaagaatttt gccgagtacc cgcaaaaatg
    28921 gcagttaaag cgcccggaca gcaacatcga tcaccgtcgg gtgcctaatc tggaaacctg
    28981 gtttacccac cacgataaaa cacgccccat cagcaagaac cccagtgact atcaagcggg
    29041 cgatattgtc tcctggcgat tagataacgg gctggcacat attggggtgg tttcagatgg
    29101 cttcgcccgc gacggtacgc cgctggtgat acataacatt ggcgcaggtg cgcaggaaga
    29161 agatgtgctg tttagctggc gcatggtggg gcattatcgt tattttgtta aataagcgat
    29221 cgccccacat gaagaaaaaa ttcagatcct ttttacacta acggcttctt ttttcttacg
    29281 gctcaccagc gccagtgcca tcacaatcag cacaataccg ctaccttcaa cggcccccgg
    29341 attctcgccg agcagccacc aggaaaagag tacgccgcaa acgggaacgg ccagtgtgct
    29401 taagctggca atactggcag gcaagttttt caatacaaac aaccataagc tccacgccag
    29461 tgccgtcgcc agaatcgcac tgtaggccag cgcccagaac acggtgggct gccagtcaat
    29521 ttcacgttgc ggcaccagta aagcgaccac actcatcacc agcgccgcat agagcatctg
    29581 ccaggatgtt aacgacaata aatccacgcg cggatgacgg gcatacagac gtttagcaac
    29641 aatcgcgctc gccccccaac tgacgccgga gaggattgcc agcatggcac ttttcatcga
    29701 agagaaatcg agttgccacg gctgcaacac caaaaataag ccgaaagcgg caatcagtat
    29761 cgcgaaatat tgcccacgtc gcaggcgttc accgagaaac aacgcggcga aaatcaccac
    29821 ccagaacggc atggtatagc ttaggatcgc caccttcccc gctccgccgc tgaccagcgc
    29881 ccactgcgcc aggccaacca tcccgcaggt ttgtaacagg gcaatggcta aggtgtattt
    29941 aaacggtgtc gggcgcattc cgcgaccacg taataaaagg acgatgaata aaacgagagc
    30001 gccgaaaatg cagcgtaagg cggtaaagtc gaaggcaccg atgtagctgg tgacttgctt
    30061 catgaaaatc cagctataac tccagataag tgtgagcaca accaggccac tgattgccag
    30121 tgggttgctc tttcctgcgg cagacatgag agatccagtg tgtagttttc ctgtggacac
    30181 tatacgccgg taaagaaact tcagcgactg cctgaaaggc agtcgctgaa gaaggtcaga
    30241 ttacagcagg tcgaaacggt cgaggttcat cactttcacc catgccgcca cgaagtcttt
    30301 aacaaacttc tcgtgagcat cgctgctggc gtaaacttca gccaccgcac gcaggacaga
    30361 gttagaacca aataccagat ccgcacggct ggcagtgtat ttcacttcgc cggtttcgcg
    30421 gtcacggcct tcaaacagct ctttcgattc gtcggtcgct ttccactcgt aacgcatatc
    30481 cagcaagttc acgaagaagt cattgctcaa tacgccaacg cggtcagtga agacgccgtt
    30541 tttgctgcca tcgaagttgg cacccagtac gcgcatcccg cccaccagcg cggtcatttc
    30601 cggcgcggtc agggtcagtt gctgggcttt gtcgatcagc agtgactcgg tggtggaaac
    30661 atccagacga gcgcgatagt tacggaaacc gtcagcaatt ggctccagca gctcgaacat
    30721 ctcaatgtca gtctgatcct gacgcgcatc aacgcggccc ggcgcaaacg gtacatgaat
    30781 gctcaaacct gcggcgcttg cggctttctc aacaccaacc acaccagcca gcacgatgat
    30841 atccgccagc gaggctttac cagactcttt ctggattttc tccagaacag gcagagcacg
    30901 aacggctgcg gcgttcacat cccagtcgcg ctgcggcatt aatgccagac gcgcaccgtt
    30961 ggcaccaccg cgtttgtcgc caccacggaa ggtagaagca gatgcccagg ccaccgatac
    31021 ccgctcacta acagacagac cagaatccgc aatcgcgaat ttcagatcga taatgtcctg
    31081 ctcggtcggg ttgtagatcg gctgcggcag cggatcttgc cagatcagat cttctttcgg
    31141 cacttccggc ccgatgtagc gagatttcgg ccccatatcc ctgtgcgtca gtttgaacca
    31201 ggcacgggca aaggcttcgt tgaacgcctg cggatcgttg aggaaacgac gagagatctt
    31261 ctcgaactca ggatcaaaac gcagcgtcag gtcggtcacc agcatcgtcg gtttacgttt
    31321 cttcgacgga tcgaatggat ccgggataat ttccggtgcg tctaccgctt cgaactggat
    31381 tgcgccagcc ggactgcggg tctgtaccca ctcatatttg aacaggtttt cgaagaaata
    31441 gttgctccac tgggtcggcg tctgggtcca gacaacttcc agaccagagg taatggcatc
    31501 tgcgccaacg ccgctgccgt aagtgctcgc ccagcctaaa ccttgttctt caatcggtgc
    31561 agcttctgga tcaggaccta cgtttgatgt cggaccggca ccgtgggttt tacccagcgt
    31621 atgaccaccc gcaatcagcg ccacggtttc ttcgtcgttc atgcccatgt tgccgaaggt
    31681 cgcgcggata gctgctgccg cagaaagcgg ttcgccgctg tgatccgggc cttccgggtt
    31741 aacgtaaatc aggcccatct cggttgcacc cagcggtgct ttcgccagcg cttccggatg
    31801 acggtgagtc agccaggctt tttcatcacc ccagttaaca tccagatccg gttcccagac
    31861 gtcttcacga ccggcaccaa aaccgaaggt acggaagccg gagttttcca gcgccacgtt
    31921 acctgcgagg ataaacaggt cggcccagga gattttctga ccatatttct gtttgattgg
    31981 ccacaacagg cgacgcgctt tatcgaggct tacgttatcc ggccaggagt tcagcggtgc
    32041 aaaacgttgc tgaccacgac ccgcgccgcc gcgtccatcg attgaacggt aagtacctgc
    32101 gccgtgccag gccatacgaa taaacagacc ggcgtagctg ccccagtcgg ctggccacca
    32161 cggctgagat tctgtcagca gggctttcag atcttttttc aggccgtagt aatctaattt
    32221 gctgaattct ttgcggtagt caaagtcctc acccagcggg ttagaacgat tagaatgttg
    32281 gtttaacagg tcaacacgga gttgatttgg ccaccagtcg cgagtggttg tgcccgcccc
    32341 cgcgctctgg tcgtgaccgc cctgatggaa cgggcatttg ccagtggctg tcgtgttatg
    32401 gatatcgtct gacgtgctca tcaatgtgct cccctctaca gtgttaccgt tacgatacac
    32461 agcgttagag agaagttata attgaattaa tccacggatg cgatagttga gatcttacat
    32521 attgttggtt aaagagatgt aggtcaaatt gatcgtaatt aagctttttt gattatgtcc
    32581 gaattcggac aaatgcttta taaaaaaggg ttttagcttc atatccttca agtaatgagg
    32641 gagaaaacaa ggctgacaac cttattttcc cgccctcatt tcgaggcagc attttgtgct
    32701 ctgtttaaaa tttgtgatca ctgtgtgatt ttcacaaaag ccacactatt tataaaccag
    32761 gtcgaacacc cagcgtatgg caaatcgcgt aactcatttc agcgcggtta agcgtataga
    32821 agtggaaatc tttcactcct tcacggctta aaatcttcac catatccatg gcaatattcg
    32881 cgcccaccag tttgcgggtt tcggcatcat catccaggcc gtcgaacatt tgcgccatcc
    32941 acgccggaat acgcacgttg gtcatatcgg caaatttctt cgcctgttta aagttagata
    33001 ccggcaaaat tcccggaata atttccacat caatgcccgc cgatacacag cggtcacgaa
    33061 aacgcaggta gctttcgaca tcgaagaaga actgagtaat cgcgcggttg gctccggcat
    33121 cgactttgcg tttcagatta agcaaatccg cctgagcgct ttttgcttcc gggtgaactt
    33181 ccggatacgc cgccacggag atatcgaaat ctgccacttc ttttaatagc gtcaccaggt
    33241 cagaagcata catttctggc ttaccacttc ccggcggcag atcgccacgc agcgccacga
    33301 tatgacgaat accgttattc cagtagtcgc gtgcaatggt gcgcaactcg tcgggcgtcg
    33361 catcaatgca agtaagatgc ggtgccgctt ccagaccagt gcgatcttta atgcctttaa
    33421 taatgctgtg cgtacggtcg cgctcgccgg agttcgcgcc ataggtcacc gatacaaact
    33481 tcggtttcag gctgctaagg cgatcgatgg agttccacag ggtctgctcc atttcactgg
    33541 tacgcggcgg gaaaaactcg aacgaaacgt taatctgccc ctggacttct gccaggctct
    33601 gattcagggc atcccgctgg ctggcgtgaa aaaagctcat accttacctc atcaatcgct
    33661 tgtagttata tgttgtgttg tgcacatcta tccgtttaga cgtccagatg taaagatgaa
    33721 ggaaaagccg aagggcgtca accgaaaaat gaccaatgat ggtgaggaaa aatcagggaa
    33781 gatgaaaaaa cttcaggatg gtaagaaaaa gaaaatccgg tatgcaaagc aaaccggact
    33841 gcaaatataa actaaccatg tacttattgc ttcttgccgg attcgtcgta aacgcttaat
    33901 ccggcctaat cctatgataa ttcaatatat ttcattagaa accataaacc cgaaaacatg
    33961 agtaccgggc attattaaat ttctgaaatt acaacaactg tgccagccgg ttgatatccg
    34021 actgaatcgc cccggcggtg acgtcgcgcc cagcgccagg cccacggatc accagcggat
    34081 tatcgcgata ccagcggctt tcgatggcaa agacgttatc gcacggcagc agtgatgcca
    34141 gcggatggtc ttcacgcacc gcttccacgc ccacacgcgc tttgccattg gcatcgaaac
    34201 gcgccacgta gcgcagcacc agccccattt cgcgggccgc ttccagccgt tgcaccatct
    34261 gctcgttaag ttcatcgcca ttttcaaaga aatggtcgat gctgccgcct tcgcaatggg
    34321 caggcaccag cgactccacg cgtacctgat ccggttcgat gttgtaacct gcttcacgcg
    34381 ccagaatcac cagcttgcgc atcacgtctt tgccagagag atcgtcacgc gggtcaggtt
    34441 cggttaagcc ctgctgccac gcctgatcca ccagctcggt aaacggcacg ctaccgtcga
    34501 attgcaggaa caaccaggag agcgtgccgg agaagatccc gctgatcgac aaaatagtat
    34561 cgccgctgtc gatcagatcg cgcacggtgt ggttgatcgg taagcccgca ccgacggtgg
    34621 cattgtacag ccagtgacgc ccggtttttt cgaaggcgtc gtggatctgg cgatatttat
    34681 tgctgtcgct ggctcccgcc agtttgttgg cgctgatgac gtggaaaccg tggctggcga
    34741 aatcaagata ctgatcagca agctgctggc tggcggtaac gtccagcacc actaaatcat
    34801 catacggatg ggcgcgcatc cacaggaaca acgactcttc atcctgctca accgcttcat
    34861 cgttgaagaa ggctaacgcg cggctggcgt ccagcccgtc atagctcaac agactgcggc
    34921 ggctgtccac cacgcctgcc agcacaaatt caaagtcggt acgtgccgaa agcgtgctct
    34981 gctcacgggc gaacagttcc agccaacggg aaccgatatt gcccttaccg aacaatacca
    35041 ggccgatgcg tttttctgcg cggaagacgg actgatgcag cccctgaatc aggctttcgg
    35101 tcgggccggt gcgcagtact gccaccaggc tgatgccgtc atcggactgc caggtaaatt
    35161 cgaccggctg gcctttcagt tgctgccaga agcggtggca atgcagcggg ttacgggtga
    35221 cgcctgcacc gaccatcgcc accagcgcca gcccctgacg caggcgcagt tcgccaggta
    35281 atcccgcttc gtcgaggatt ttcagcgcac tgtcggccac ttctgaggtg tagcaaaatt
    35341 gcagcaactg gcgatcgtta tgtacgccaa ccgccagcgg gcgtacctgc gcgcgtttca
    35401 ggatttggtc gatctcttta tgcgccagtt tgaaatcctg actggcgggc acctgaaact
    35461 caatcaaaca gacatcatcg tggctggtga caatacgcgc accagtaccg gaggccagca
    35521 cgcgttcaat gcgcgtggaa ccttgatccg gcgtgtagct acagcgcagt tgcaggtcga
    35581 tttcgctgcc agaaaccggc tgtaaagtac gggcgtgaag aacgggagcc gccagacgag
    35641 ccagttcgct ggcttcatcc agacgcagca acggcagcag acaggcatct ttcactttac
    35701 gcgggtcggc actgtatacc ccggcgacgt cgctccagat ggttacacga gaaacacccg
    35761 ccagcgcacc gatttgtgtc gcggaatagt cggaaccgtt acgccccagc agcaccgttt
    35821 caccggcgtt gttacggctg ataaacccgg tcaccaccag acgtttgccc ggatgttgca
    35881 ccagtagctg ttgcagcaac gggtaagaaa gcccttcatc aacctgcggt tgtgcggcgc
    35941 gttcagcgcg taaaaactcg cgggcatcaa gccaggccgc tggcagccct tgttgattaa
    36001 gtaccgcaga catcaggcgt gccgaccata cttccccgtg gcccaccact tccgcataca
    36061 ctgcgtcgtt aataccgctg tcgagcagcg ccgccaggcg ttccagatcg ctgacaaaag
    36121 cgctaatgag gctatcggct tcttcagcgg gtagcagacc gctaatcaga tcgcactgat
    36181 agcgacgcag cgtttgttga acctgatgcg cagagagacg atcggtctgg cttagtttca
    36241 accagttaat caactggtta gtggtgctac cggcggcgga aaccaccatc atatcgtcag
    36301 gctgagagta ctccgccata atgcccgcga cacgcaaata acacttcaca tcagccagac
    36361 tactgccacc aaatttatgc agctgacgac ctttcgcccc tgcctgcgca atcacactca
    36421 tttttacccc ttgtttgcag cccggaagcc attttccagg tcggcaatta aatcttcgcc
    36481 atcttcaata ccggtggaga tacgcagcag cgtctcggag atcccgacgg cagcacgcgc
    36541 ttctggtgcc atgcctgcat gtgtcatggt tgcggcgtga gagattaaac tttccactcc
    36601 ccctaatgat tccgccagcg taaacaacga cagcccgctc aggaaacgac gcagcgtctg
    36661 ctcatcgcca tccagttcaa aactcaacat tgcgccaaag cctttttgct gacgcgcggc
    36721 aatttcatgc ccctgatttt ccggcagcga cgggtgatac agttttttca ccaacggctg
    36781 ggtttgcaga tatttcacaa tcgcctgcgc gttgcgctgc gccagctcca tacgcggcac
    36841 cagcgttcgc aacccgcgta gcagcagata gctgtcaaac gcgccgcccg tcacgccaat
    36901 attgtttgcc caccaggcaa gttcagtgac aacgtccggg tctttagcaa tcaccacgcc
    36961 agccaccacg tcagagtgac cgttcagata tttcgtgcat gaatgcaaca ccagatcggc
    37021 acctaatgcc agcggatttt gtaatgccgg gcttaagaag gtgttatcca ccacgctcac
    37081 cgccccgact tcccttgcca gatggcagat tttcgcaata tccacgacgc gtaacaatgg
    37141 attacttggg ctttctacca gtaccagttt gggtttttct gccagcgctg cccgtaatgc
    37201 ctgttcatcg ccttgatcaa caaacaacac gcgatagcaa ccgcgtttcg ccagactgtc
    37261 gaacaggcga tagctaccgc cgtagcagtc gtgcggcgca accagcagat cgccaggttt
    37321 caaaaagacg gtcgttacca ggtgaatcgc ggacatgccg gtattagtaa gtactgcgcc
    37381 agcaccgcct tccagttctg ccagcgcacg ctgaaccaca tcgcgcgttg ggttgccgcg
    37441 acgcgagtaa tcatgcgcgc gcggttcatt aaatccggta aagttatagg tgctggaaag
    37501 atggatggga gggacaacgc aaccatactg ttcgtcgtca tttaacccgc tacgcactgc
    37561 gatggtggcc tgtttacgcg tcatgtgatg aagtttcctg ggctttgtcg gtgaaatgtc
    37621 aggcaccaga gtaaacattg tgttaatgga cgtcaataca tctggacatc taaacttctt
    37681 tgcgtataga ttgagcaaat cccaaatagc cgttaaaatt atatgcatta tcacgccgac
    37741 aggtgcatta cacgatgtca cggtaacgcc tgtacggtaa actatgcggg tttacggtca
    37801 gtacccacat aaattgtgtg gtctggtctc aatttattga cgaagaggat taagtatctc
    37861 atggctgaat ggagcggcga atatatcagc ccatacgctg agcacggcaa gaagagtgaa
    37921 caagtcaaaa agattacggt ttccattcct cttaaggtgt taaaaatcct caccgatgaa
    37981 cgcacgcgtc gtcaggtgaa caacctgcgt cacgctacca acagcgagct gctgtgcgaa
    38041 gcgtttctgc atgcctttac cgggcaacct ttgccggatg atgccgatct gcgtaaagag
    38101 cgcagcgacg aaatcccgga agcggcaaaa gagatcatgc gtgagatggg gattaacccg
    38161 gagacgtggg aatactaaag caaaaaagag cggcgcggag tggaatcgcc tgatgcgcta
    38221 cgcttatcag gcctacgtca tattgcaatt tattgaattt gcacgaactt gtaggccgga
    38281 taaggcgttc acgccgcatc cggcataaac aacgagcacg ttgtctgcga cccaccgctt
    38341 tttatacatg gacgtttaac tatgaaaaac aggctgctga tcctcagcct gctggtttct
    38401 gtacctgcct ttgcctggca gccacaaacc ggcgacatca tctttcagat ctctcgctca
    38461 tcgcaaagta aagcgatcca actggcgacc cattccgatt atagccacac cggtatgctg
    38521 gtgatacgca acaaaaagcc ctacgttttt gaagcggtcg gcccggtgaa atacaccccg
    38581 ctcaagcagt ggatcgccca tggtgaaaag ggcaaatacg ttgttcgccg cgttgaaggc
    38641 ggactgagtg ttgaacaaca gcaaaaactg gcgcaaacgg caaaacgtta tctcggtaaa
    38701 ccgtatgatt tcagcttctc atggagcgac gatcgccagt actgttcgga agtggtgtgg
    38761 aaggtttacc agaacgcgct gggaatgcgc gtgggcgagc agcagaagtt gaaagagttc
    38821 gatctcagca acccgctggt ccaggctaag ctcaaagaac gctacggcaa aaatattccg
    38881 cttgaggaaa cggtagtttc accacaagcc gttttcgacg cgccacaact caccacagtc
    38941 gccaaagagt ggccgctgtt ttcgtggtaa tggatgatga aaaaaataat tcccgccgta
    39001 gtctgtttgt tgctttgtag ttgcacgacc tttccgcaaa ctgtcgctcc ggtaaacggt
    39061 gatgcctcca attagttgaa ttaaggatat aatactcgct tttaaggttg tttcatggtc
    39121 acagttaacc ttcattgccc ccgttgccag tccgtccagg tttaccgtca cgggcagaac
    39181 ccaaaaagac atgagcgatt ccgcggccgt gactgtcacc gggtatttca gctcagttac
    39241 tgttacgagc cccgtaaacc gggagttaaa gaccaaatcg ctgaaatgac tttcaacggt
    39301 gccggggttc gcgatactgc ccgtacgctg aaaatcggca ttaatacagt catccgaact
    39361 ttaaaaaact ctcgccacga agaataacgt catccccggt cgctcatgct gatgtggcac
    39421 tcatctgcaa acttagtcag caatggagct ttgtcggcag taaagcgcga cagcactggc
    39481 tctggtacgt gtacaacacg aaaactggtg gtgttctggc ctacactttt ggcccccgga
    39541 ctgacgaaac ctgtcgtgaa ttgcgggcgc tgcttaccct tttaccatcg gcatgttgac
    39601 cactgacgaa tggggtagtt acgctagaga gttaccgaag gagaagcatc tgactggtaa
    39661 gatttttact cagcacattg agcgtaacaa tctgaccctg agaacccgaa tcaaacgtct
    39721 ggcccgcaga accatctgtt tctcgcgttc tgtagaactt catgaaaaag tcatcggtgc
    39781 ttttatcgaa aatatatgtt ctactaattg gagtcatcac ccttctgata agctttcctt
    39841 ggatgattac cagactgaat acacagccaa agatttttac ctcattctgc tcttttttct
    39901 caaatgacat atattatatt ttacatcata ttcataggca tatgacattc ccccgccccc
    39961 cattatgtca ttgtgggttg atgttgggct ttcataaaaa aaacatcttt ttttgaacta
    40021 gcattaatta tatttacttt atatattaaa atattttctc catctggaaa tattttagcc
    40081 acacaatata aagcagctga atccgaatta cttaaaaata tattatcatc acccataact
    40141 ccagccatta aatgactgga gactaaagca aggaaataac aacattgtat tgccttcatt
    40201 ttaattactc ccttgcctga taattgtaac tccattccct ttagctttgg gattatattt
    40261 tagataggtg ttatttggtg ttcccaagta actactgtat tctttttttc ccattccatt
    40321 catataagaa ttcgttaaat ctttgcttga aaaatttaga ctatcataaa catcattttc
    40381 ttttgttact ttatttcctt tatcgtcaga gtaaaaaccg tgagtgtgat aatcgcctac
    40441 tctctctgag ccttcagggc attttatatt tcgcaagtct acactatcat tatcatttga
    40501 acttatcgga ttcatgggga aatattcacc ttgcttctta catattagtc caccatattc
    40561 tttattctcg ttaattgatt ctccattaca cattgccaat gcaaatattc caatatcatc
    40621 aatatttttt gatttaaatt catataatcc taatggatcc atgctattca ccgggctcag
    40681 cggatatgta tacaggttcc atcccccctt cagcccaatc ggatcctgag tgatataccg
    40741 cccctgcagc gggtcataat agcggtggcg gttataatac aggccggact cctcatcata
    40801 ctgctgcccc ggcaaacgaa taagctgctg cagctgatgc gggttctctt cattcagcag
    40861 gttgccccat tcatcatatt cccggcgtat ttccagcccg tcaggcccgg tggctgacgt
    40921 taactggctg tggtggttat agtaaaacgc cgatgccctg ccatccggcg tggtgatgcg
    40981 cgtgatgaga cccgtcacca catccgggct gtattctgtt gtccggcctg cggcatccgt
    41041 ctgtgtcctg agcctgccca cggcgtcaaa ctgactctgc gtgacgctgc cgtccgcgtg
    41101 ttcctttttc accacccgct tcagcccggc ttcaccctac gtgtg
//
LOCUS       DAESHI010000027        41011 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.027, whole genome shotgun sequence.
ACCESSION   DAESHI010000027 DAESHI010000000
VERSION     DAESHI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41011
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.027"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            216..1880
                     /gene="asnB"
                     /locus_tag="KW011_001462"
     CDS             216..1880
                     /gene="asnB"
                     /locus_tag="KW011_001462"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HBH7659839.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            2260..2336
                     /locus_tag="KW011_001463"
     tRNA            2260..2336
                     /locus_tag="KW011_001463"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2294..2296,aa:Met,seq:cat)
     gene            2345..2429
                     /locus_tag="KW011_001464"
     tRNA            2345..2429
                     /locus_tag="KW011_001464"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2379..2381,aa:Leu,seq:tag)
     gene            2453..2527
                     /locus_tag="KW011_001465"
     tRNA            2453..2527
                     /locus_tag="KW011_001465"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2485..2487,aa:Gln,seq:ttg)
     gene            2562..2636
                     /locus_tag="KW011_001466"
     tRNA            2562..2636
                     /locus_tag="KW011_001466"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2594..2596,aa:Gln,seq:ttg)
     gene            2652..2728
                     /locus_tag="KW011_001467"
     tRNA            2652..2728
                     /locus_tag="KW011_001467"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2686..2688,aa:Met,seq:cat)
     gene            2777..2851
                     /locus_tag="KW011_001468"
     tRNA            2777..2851
                     /locus_tag="KW011_001468"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2809..2811,aa:Gln,seq:ctg)
     gene            2888..2962
                     /locus_tag="KW011_001469"
     tRNA            2888..2962
                     /locus_tag="KW011_001469"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2920..2922,aa:Gln,seq:ctg)
     gene            complement(3116..4291)
                     /gene="ubiF"
                     /locus_tag="KW011_001470"
     CDS             complement(3116..4291)
                     /gene="ubiF"
                     /locus_tag="KW011_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HBH7659840.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            4437..5861
                     /gene="miaB"
                     /locus_tag="KW011_001471"
     CDS             4437..5861
                     /gene="miaB"
                     /locus_tag="KW011_001471"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBH7659841.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            6014..7054
                     /locus_tag="KW011_001472"
     CDS             6014..7054
                     /locus_tag="KW011_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBH7659842.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            7051..7518
                     /gene="ybeY"
                     /locus_tag="KW011_001473"
     CDS             7051..7518
                     /gene="ybeY"
                     /locus_tag="KW011_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBH7659843.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            7608..8486
                     /gene="corC"
                     /locus_tag="KW011_001474"
     CDS             7608..8486
                     /gene="corC"
                     /locus_tag="KW011_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HBH7659844.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            8511..10049
                     /gene="lnt"
                     /locus_tag="KW011_001475"
     CDS             8511..10049
                     /gene="lnt"
                     /locus_tag="KW011_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HBH7659845.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(10127..12004)
                     /locus_tag="KW011_001476"
     CDS             complement(10127..12004)
                     /locus_tag="KW011_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBH7659846.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            12459..13367
                     /gene="gltI"
                     /locus_tag="KW011_001477"
     CDS             12459..13367
                     /gene="gltI"
                     /locus_tag="KW011_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HBH7659847.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            13537..14277
                     /gene="gltJ"
                     /locus_tag="KW011_001478"
     CDS             13537..14277
                     /gene="gltJ"
                     /locus_tag="KW011_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HBH7659848.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            14277..14951
                     /gene="gltK"
                     /locus_tag="KW011_001479"
     CDS             14277..14951
                     /gene="gltK"
                     /locus_tag="KW011_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HBH7659849.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            14951..15676
                     /gene="gltL"
                     /locus_tag="KW011_001480"
     CDS             14951..15676
                     /gene="gltL"
                     /locus_tag="KW011_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HBH7659850.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            15794..16729
                     /gene="rihA"
                     /locus_tag="KW011_001481"
     CDS             15794..16729
                     /gene="rihA"
                     /locus_tag="KW011_001481"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HBH7659851.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            16821..18491
                     /gene="hscC"
                     /locus_tag="KW011_001482"
     CDS             16821..18491
                     /gene="hscC"
                     /locus_tag="KW011_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HBH7659852.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(18551..20002)
                     /gene="djlC"
                     /locus_tag="KW011_001483"
     CDS             complement(18551..20002)
                     /gene="djlC"
                     /locus_tag="KW011_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HBH7659853.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(19999..20706)
                     /locus_tag="KW011_001484"
     CDS             complement(19999..20706)
                     /locus_tag="KW011_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBH7659854.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            20808..21362
                     /locus_tag="KW011_001485"
     CDS             20808..21362
                     /locus_tag="KW011_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBH7659855.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(21372..22799)
                     /locus_tag="KW011_001486"
     CDS             complement(21372..22799)
                     /locus_tag="KW011_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HBH7659856.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(22796..23503)
                     /locus_tag="KW011_001487"
     CDS             complement(22796..23503)
                     /locus_tag="KW011_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBH7659857.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQP
                     LADYPTCPETLKDMSDL"
     gene            23667..24644
                     /locus_tag="KW011_001488"
     CDS             23667..24644
                     /locus_tag="KW011_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HBH7659858.1"
                     /translation="MVFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(24714..25196)
                     /locus_tag="KW011_001489"
     CDS             complement(24714..25196)
                     /locus_tag="KW011_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HBH7659859.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            25431..28013
                     /gene="leuS"
                     /locus_tag="KW011_001490"
     CDS             25431..28013
                     /gene="leuS"
                     /locus_tag="KW011_001490"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBH7659860.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            28028..28609
                     /gene="lptE"
                     /locus_tag="KW011_001491"
     CDS             28028..28609
                     /gene="lptE"
                     /locus_tag="KW011_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HBH7659861.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            28609..29640
                     /gene="holA"
                     /locus_tag="KW011_001492"
     CDS             28609..29640
                     /gene="holA"
                     /locus_tag="KW011_001492"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBH7659862.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIVPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            29642..30283
                     /gene="nadD"
                     /locus_tag="KW011_001493"
     CDS             29642..30283
                     /gene="nadD"
                     /locus_tag="KW011_001493"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBH7659863.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            30307..30918
                     /locus_tag="KW011_001494"
                     /pseudo
     CDS             30307..30918
                     /locus_tag="KW011_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            31178..31495
                     /gene="rsfS"
                     /locus_tag="KW011_001495"
                     /gene_synonym="rsfA"
     CDS             31178..31495
                     /gene="rsfS"
                     /locus_tag="KW011_001495"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBH7659864.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            31499..31966
                     /gene="rlmH"
                     /locus_tag="KW011_001496"
     CDS             31499..31966
                     /gene="rlmH"
                     /locus_tag="KW011_001496"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBH7659865.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            31997..33898
                     /gene="mrdA"
                     /locus_tag="KW011_001497"
     CDS             31997..33898
                     /gene="mrdA"
                     /locus_tag="KW011_001497"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HBH7659866.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            33901..35013
                     /gene="mrdB"
                     /locus_tag="KW011_001498"
                     /gene_synonym="rodA"
     CDS             33901..35013
                     /gene="mrdB"
                     /locus_tag="KW011_001498"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HBH7659867.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            35024..36112
                     /gene="rlpA"
                     /locus_tag="KW011_001499"
     CDS             35024..36112
                     /gene="rlpA"
                     /locus_tag="KW011_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HBH7659868.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            36251..37462
                     /gene="dacA"
                     /locus_tag="KW011_001500"
     CDS             36251..37462
                     /gene="dacA"
                     /locus_tag="KW011_001500"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HBH7659869.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            37572..37835
                     /locus_tag="KW011_001501"
     CDS             37572..37835
                     /locus_tag="KW011_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HBH7659870.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            37936..38577
                     /gene="lipB"
                     /locus_tag="KW011_001502"
     CDS             37936..38577
                     /gene="lipB"
                     /locus_tag="KW011_001502"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HBH7659871.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            38837..39790
                     /locus_tag="KW011_001503"
     CDS             38837..39790
                     /locus_tag="KW011_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBH7659872.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            39999..40964
                     /gene="lipA"
                     /locus_tag="KW011_001504"
     CDS             39999..40964
                     /gene="lipA"
                     /locus_tag="KW011_001504"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HBH7659873.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT        10295 a        10449 c        10293 g         9974 t
ORIGIN      
        1 ccccgtaaaa tataaccatt caataaaacc cgctaaaaat tatcgattca tcaataaata
       61 catcctcaca ccagcccctt tttcaccaat cgacaatcac cattacgttt tttatttttt
      121 caccgccaaa tcgcttgcgc acagtgctgc tttgcggcat ttttttaaac aagcaaacac
      181 aacaagcaac aaataccagg ttaacggaga aggttatgtg ttcaattttt ggcgtattcg
      241 atatcaaaac agacgcagtt gagcagcgta agaaagcact cgagctgtca cgcctgatgc
      301 gtcatcgtgg cccggactgg tccggtattt atgccagcga taacgccatt ctcgctcacg
      361 aacgtctgtc aattgttgac gttaacgcgg gggcgcaacc tctctacaac caacaaaaaa
      421 cccacgtact ggcggtaaac ggtgaaatct acaaccacca ggcattgcgc gctgaatatg
      481 gcgatcgtta tcagttccag acagggtctg actgtgaagt gatcctcgcg ctgtatcagg
      541 aaaaagggcc ggaatttctc gacgacttgc agggcatgtt tgcctttgcc ctgtacgaca
      601 gcgaaaaaga tgcctacctg attggtcgcg accatctggg gatcatccca ctgtatatgg
      661 ggtatgacga acacggtcag ctgtatgtgg cctcagaaat gaaatcgctg gtgccagttt
      721 gccgcacgat taaagagttc ccggcgggga gctatttgtg gagccaggac ggcgaaatcc
      781 gttcttatta tcatcgcgac tggttcgact ttgatgcagt gaaagataac gtgaccgaca
      841 aaaacgagct gcgtcaggct ctggaagatt ccgttaaaag ccatctgatg tctgatgtgc
      901 cttacggtgt gctgctttct ggtggtctgg attcctcaat tatttccgct atcaccaaga
      961 aatacgcagc ccgtcgcgtg gaagatcagg aacgctctga agcctggtgg ccccagttac
     1021 actcctttgc tgtaggtctg ccgggttcac cggatcttaa agcagcccag gaagtggcaa
     1081 accatctggg cacggtgcat cacgaaattc acttcactgt acaggaaggt ctggatgcca
     1141 tccgcgacgt gatttaccac atcgaaactt atgatgtgac cactattcgc gcttcaacac
     1201 cgatgtattt aatgtcgcgt aagatcaagg cgatgggcat taaaatggtg ctgtccggtg
     1261 aaggttctga tgaagtgttt ggcggttatc tttacttcca taaagcgccc aacgctaaag
     1321 aactgcatga agagacggtg cgtaaactgc tggccctgca tatgtatgac tgcgcgcgcg
     1381 ccaacaaagc gatgtcagcc tggggcgtgg aagcacgcgt tccgttcctc gacaaaaaat
     1441 tccttgatgt ggcgatgcgc attaacccgc aggataaaat gtgcggtaac ggcaaaatgg
     1501 aaaaacacat cctgcgtgaa tgttttgagt catacctgcc cgcaagcgtg gcctggcggc
     1561 agaaagagca gttctccgat ggcgtcggtt acagttggat cgacaccctg aaagaagtgg
     1621 cggcgcagca ggtttctgat cagcaactgg aaactgcccg cttccgcttc ccgtacaaca
     1681 cgccaacctc aaaagaagcg tatctgtacc gggagatctt tgaagaactg ttcccgcttc
     1741 cgagcgccgc tgagtgcgtg ccgggcggcc cgtccgtcgc gtgttcttcc gctaaagcga
     1801 ttgagtggga tgaggcgttc aagaaaatgg acgatccgtc tggtcgtgcg gttggcgttc
     1861 accagtcggc gtataagtaa gaaatgcgaa acagccccgg tgaaatccgg ggctgtttca
     1921 gttattgagg tgcttgttga ttctgatatg cagtaaggaa atgttccggg tttgccacat
     1981 cactgtttga caactcatca ctgtaggcca gtatttcata gattaatttc ccaccaccat
     2041 tgttgaattc cgaatttcat cctgtatgac aaaagaatgg ctgtagggtg gggaaaaaac
     2101 acaaagtaaa caataattga cgaatatagc gccacgctgt tcgcaaccta accaaacagt
     2161 cactttcgag caattttcct tgaaaaagag gttgacgctg caaggctcta tacgcataat
     2221 gcgccccgca acgccgataa ggtatcgcga aaaaaagatg gctacgtagc tcagttggtt
     2281 agagcacatc actcataatg atggggtcac aggttcgaat cccgtcgtag ccaccatctt
     2341 ttttgcggga gtggcgaaat tggtagacgc accagattta ggttctggcg ccgcaaggtg
     2401 tgcgagttca agtctcgcct cccgcaccat tcaccagaaa gcgttgtacg gatggggtat
     2461 cgccaagcgg taaggcaccg gtttttgata ccggcattcc ctggttcgaa tccaggtacc
     2521 ccagccatct tcttcgagta agcggttcac cgaccggtta ttggggtatc gccaagcggt
     2581 aaggcaccgg tttttgatac cggcattccc tggttcgaat ccaggtaccc cagccatcga
     2641 agaaacaatc tggctacgta gctcagttgg ttagagcaca tcactcataa tgatggggtc
     2701 acaggttcga atcccgtcgt agccaccaaa ttctgaatgt gtcgaatacg ttcggcaaat
     2761 tcaaaaacca atttgttggg gtatcgccaa gcggtaaggc accggattct gattccggca
     2821 ttccgaggtt cgaatcctcg taccccagcc aatttattca agacgcttac atcgtaagtg
     2881 caccagttgg ggtatcgcca agcggtaagg caccggattc tgattccggc attccgaggt
     2941 tcgaatcctc gtaccccagc cacattaaaa aagctcgctt cggcgagttt tttgcttttc
     3001 tgcgttcatt caatgtcgaa tgcgatgttg acacgtctta tccttcaatg tcggatgcga
     3061 cgctgccgcg tcttatccga cctacggttg gcacgcatcc ggcaatgttg taaggctaca
     3121 accctaacgc atatttcagc gcctgacgtt tcaacacgcc agcacgctct gccgccatta
     3181 acccgagatt acgcacaaaa cgcagcggcg gcagattatt actgaatccg gcataaaaca
     3241 gatccatacc actttgcata atgaagttat ccgccatgcg ccgcatctgg taacgcttga
     3301 ggacaggata actggcccac gcttcaccgt agctgcgggc attcaccaga acatcaatca
     3361 gggcatcgac atcacgataa ccaagattca ccccctgccc cgccagcgga tggatggtat
     3421 gcgcggcatc gcccaccagc gcaagccccg gctgcacata ctgcaacgca tgtcggcgcg
     3481 tcagcggaaa cgcaccagcg gcaagcggtg taacgtaacc cagacgcgac gggaaatgct
     3541 tcgcgatttc cgcctggaat tgcgccatat tcatattctg caactggcga atacgcgccg
     3601 gagagtcata ccacaccagc gatgcccagt tatcaaacaa cggcagaaac gcacgcggtc
     3661 cgtccggggt aaattgctgc caggtgctgt cgccgggatc gttctcacac tgtacgctaa
     3721 tcaacatgca cgactgcgca tactgccagg catgaacgcc aattcccgcc atctgccgca
     3781 cctgagaatt tgcgccgtcg gcaccaatca ccagtttcgc gcggatcgtt tcaccgccct
     3841 tcagttccag ctcctgaaga tcattatctc gatgtaatgc aatcagcgag cctggcacac
     3901 gtaacgttac tttcggatgc gcttccagcg cctgccacag cgcctgttgc aggacggtgt
     3961 tttccaccat atagccaagc aacggtagct taagttcagc ggcgtcaaac accacatgcg
     4021 ccgtttccca ctcccacgtt tccagtctgc ggtaaggatg gcaacgcata gcctgtactg
     4081 catcccagac ccctaaccct ttaagcaatg ataccgatgc ggcgctgatc gccgagatcc
     4141 gcacgtccgg ttggctatca gcgacaaacg gtgcaggttc tgcgtgctcg atcaccgtta
     4201 ccgaaaatcc gtgctgtgcc agccccagcg ccagcgcgcc gccgaccatt cctccgccga
     4261 caatggcaat ttccgttggt tgatttgtca tggtcaatca tcctgttagc aatatgcttt
     4321 aagtttacag gatttttacc cccaagggct gataacgctc atactggtca caacggcagc
     4381 aaagcattac actatacgcc ctgcattcct ggctactatt tcgcaagagc aagtcgatga
     4441 ccaaaaaact ccatattaaa acctggggct gtcagatgaa cgagtacgat tcatcgaaga
     4501 tggccgatct gctggatgcc acccacggct atcaactgac cgacgtggcg gaagaagcgg
     4561 atgtgctgct gctgaacacc tgctcaatcc gcgagaaggc tcaggaaaaa gtcttccatc
     4621 agttgggtcg ctggaaactg ttaaaagaga agaatccaga cctgattatc ggcgtcggtg
     4681 gctgcgtggc atcgcaagaa ggcgagcaca ttcgccagcg cgcccactat gtcgatatta
     4741 tttttgggcc gcaaacgctg caccgtctgc cggagatgat caactccgtg cgcggcgacc
     4801 gcagcccggt tgtagatatc agcttcccgg aaatcgagaa gtttgaccgt ctaccggaac
     4861 cgcgcgccga agggccgacc gcgtttgtct ccatcatgga aggctgcaat aaatattgca
     4921 cctactgcgt ggtgccttac acccgtggtg aagaggtaag ccgtccgtct gacgatattc
     4981 tgtttgagat tgcccagctt gcggctcagg gcgtgcgtga agtcaacctg ctcggtcaga
     5041 acgtgaacgc ctggcgtggt gaaaactacg acggctccac cggatcgttt gccgatctgc
     5101 tgcgtctggt tgctgcgatc gacgggatcg atcgtattcg ctttaccacc agccatccga
     5161 tcgaattcac cgacgatatc atcgaagtgt atcgcgacac gccggagctg gtgagcttcc
     5221 tgcatctgcc ggtacaaagc ggttccgatc gcattctgaa cctgatgggc cgtactcaca
     5281 cggcgctgga gtacaaagcg atcatccgta aactgcgtgc ggcgcgtccg gatattcaga
     5341 tcagttctga cttcattgtt ggcttccctg gcgaaaccac cgaagacttc gagaaaacga
     5401 tgaagctgat tgccgacgtc aatttcgaca tgagctacag ctttatcttc tctgcacgtc
     5461 cgggtacacc agccgccgat atggttgatg atgttccgga agaagagaag aagcaacgtc
     5521 tgtatattct gcaagaacgt atcaatcagc aagcgatggc atggagccgc cggatgctcg
     5581 gcaccaccca gcgtattctg gtagaaggga cttcgcgtaa gagcatcatg gagctttccg
     5641 ggcgtaccga aaataaccgc gtggtcaact tcgagggcac accggatatg atcggtaaat
     5701 tcgtcgatgt agaaattacc gacgtctacc cgaactctct gcgcggtaaa gtggtgcgta
     5761 ctgaagatga aatgggtctg cgcgtggcag aaacaccgga atcggtgatt gcccgtaccc
     5821 gcaaagaaaa cgaccttggc gtgggttatt atcagccgta attctcaggc ccgccgttcc
     5881 ggtgggcctt gttttttctc tctttatccc catctttatc gcaatgcttg cgcctttttc
     5941 ctgtggcgtg aataatttcc ttataggccg gtgaatgttc agcttccggc ggcatacaga
     6001 taagaggaac ggtttgaaca tagacactcg cgaaatcacc ctggagccag cagacaacgc
     6061 gcgtctgttg agcctgtgcg gcccgtttga tgacaacatc aagcagctcg aacgccgtct
     6121 cggcatcgag atcaatcgcc gcgacaacca ctttaaactg accggccgtc cgatttgcgt
     6181 caccgctgcg gcagacattc tgcgtagcct gtatgtcgat acagccccga tgcgcggtca
     6241 gattcaggat atcgaaccgg aacagatcca ccttgcgatt aaagaagcgc gagtactgga
     6301 gcaaagtgcg gagagcgtgc cggagtacgg caaagcggtc aatatcaaaa ccaaacgcgg
     6361 cgtaattaag ccgcgcacgc caaaccaggc gcagtacatc gccaatattc tcgaccatga
     6421 cattaccttc ggcgttggcc cggcgggtac gggtaaaacc tacctggcag tggctgcggc
     6481 agttgatgcc ctggagcgtc aggagattcg ccgtattctg ctgactcgtc cggcggtcga
     6541 agccggtgag aaactgggct tcctgcctgg cgatttaagc cagaaagtag acccgtatct
     6601 gcgcccactg tacgacgcgc tgtttgaaat gctgggcttt gagaaagttg agaaactgat
     6661 tgagcgcaac gttattgaag tcgcgccgct ggcctatatg cgtggtcgta cgctgaacga
     6721 cgcgtttatc attctcgatg agagccagaa caccaccatc gaacagatga agatgttcct
     6781 gacccgtatc ggttttaact caaaagcggt tatcaccggc gacgtcacac agatcgacct
     6841 gccgcgtaat actaaatcag gcttacgtca cgccatcgaa gtgctggccg atgtcgaaga
     6901 gatcagcttt aacttcttcc acagcgaaga cgtggttcgt caccctgtgg tggcgcgtat
     6961 cgttaacgcc tatgaagcct gggaagaagc cgaacaaaaa cgaaaagcgg cgctggcggc
     7021 agaacgcaag cgcgaagaac aggaacaaaa atgagtcagg tgatcctcga tttacaactg
     7081 gcatgtgaag ataattccgg gttaccggaa gagagccagt ttcaaacatg gctgaatgcg
     7141 gtgatcccgc agtttcagga agaatcggaa gtgactattc gcgtggtcga taccgccgaa
     7201 agccacagtc tgaatctgac ctatcgcggt aaggataagc cgaccaacgt gctctccttc
     7261 ccgtttgaag tgccgcctgg catggaaatg tcgctactgg gcgatctggt tatctgccgt
     7321 caggtggttg agaaggaagc tcaggagcaa ggcaaaccac tggaggcgca ctgggcgcat
     7381 atggtggtgc acggcagtct gcatttgtta ggttacgatc acatcgaaga tgacgaagca
     7441 gaagaaatgg aagccctcga aacagagatt atgcttgctc tgggctatga ggatccgtac
     7501 attgccgaga aagaataatt tgccaggcat ttgactggca gacgtccccg ccgttggtga
     7561 ttaacgacgg cgttttgatt aactaacatg agaacccata cgacgccatg agcgacgaca
     7621 attcacacag tagtgacacg ataagcaaca agaagggatt tttctccctg ttactcagcc
     7681 aacttttcca cggtgaaccg aaaaaccgtg acgaactgct ggcgctgatc cgtgattccg
     7741 ggcagaacga ccttatcgac gaagatacgc gcgatatgct cgaaggggtg atggacatcg
     7801 cagaccaacg cgttcgcgac atcatgatcc cccgctccca gatgattacc ctgaaacgca
     7861 accagacgct ggacgaatgc cttgatgtca tcatcgagtc cgcccactca cgtttcccgg
     7921 tgattagcga agacaaagat cacattgaag ggattctgat ggcgaaagac ttgctgccgt
     7981 ttatgcgcag cgatgctgaa gccttcagca tggacaaagt gttacgtcag gcggttgtcg
     8041 ttcctgaaag taagcgcgta gaccggatgc tgaaagagtt tcgctctcag cgttaccaca
     8101 tggcgatcgt cattgacgaa ttcggtgggg tttccggtct ggtgaccatt gaagacatcc
     8161 tggaactgat tgttggtgag attgaagacg agtatgacga agaagatgat atcgacttcc
     8221 gtcagctgag tcgtcatacc tggaccgtgc gtgcactggc ttccattgaa gacttcaacg
     8281 aagcgttcgg cacccacttt agcgatgaag aggttgacac tatcggtggt ctggtgatgc
     8341 aggcatttgg gcatcttccg gcgcgtggcg aaactatcga catcgacggt taccagttca
     8401 aagtggcgat ggccgacagt cggcgtatta ttcaggttca tgtcaaaatc ccggatgact
     8461 caccccagcc gaagctggat gaataaaacc gaaactggat agataactac atggcttttg
     8521 cctcattaat tgaacgccag cgcattcgcc tgctgctggc gttattattc ggtgcctgcg
     8581 gaacgctggc cttctctcct tacgacgtct ggcctgcggc gattatttcg ttgatggggc
     8641 ttcaggcgtt gacctttaac cgccgtccac tccagtctgc cgctattggc ttttgctggg
     8701 gatttggcct ctttggcagc ggtattaact gggtctatgt cagcatcgcg acctttggcg
     8761 gaatgcctgg cccggttaac atcttcctgg tggtgctgct ggcggcgtat ttgtcgctgt
     8821 ataccggact gtttgctggc gtgctgtcgc gtctgtggcc gaaaaccacc tggctgcgcg
     8881 tggcgattgc cgcccctgct ctctggcaag tgaccgagtt tctgcgcggt tgggtactga
     8941 caggcttccc gtggttacag ttcggctata gccagattga tggcccgtta aaagggctgg
     9001 caccgataat gggcgtggaa gccattaact tcctgctgat gatggttagt ggcctgctgg
     9061 cactggcgtt ggtcaaacgc aactggcgtc cgctggtggt ggccgtcgtg ctgtttgccc
     9121 tacccttccc gctgcgttac atccaatggt ttaccccgca accggagaaa accattcagg
     9181 tttcgatggt tcagggcgat attccgcaat cactgaaatg ggatggagat cagcttctta
     9241 atacgctgaa gatttactac aacgcaacgg ctccgctgat gggcaaatca tcgttgatta
     9301 tctggccgga gtcggcgata accgatctgg aaattaatca gcaaccgttc ctcaaagcac
     9361 tggacggtga gttgcgcgat aaaggtagct cgctggtaac cgggattgtc gacgcgcgtc
     9421 tcaataagca gaaccgctac gatacctaca acaccatcat cacactgggt aaaggtgcac
     9481 catacagcta cgaatcagcc gatcgctata acaaaaacca tctagtgccg tttggcgagt
     9541 ttgtcccgct ggagtcgatt ctgcgtccgt tagcaccgtt ctttgatctg ccgatgtcgt
     9601 cgttcagccg tgggccatat atccagccgc cgctgtcggt aaatggtatt gagcttactg
     9661 cggctatttg ctacgagatc attctcggcg agcaagtgcg cgataacttc cgcccggata
     9721 ccgactatct gctgactatc tccaacgatg cgtggtttgg taagtctatt ggtccatggc
     9781 agcacttcca gatggcgcga atgcgtgcgc tggagctggc gcgcccactg ttgcgcagca
     9841 ccaacaacgg cattacggcg gtgattggcc cgcaggggga ggttcaggcg atgatcccgc
     9901 agttcacccg cgaggtgttg accactaatg tgacaccgac caccggactc acaccgtatg
     9961 cacgtaccgg caactggcca ctgtgggtgc tgacggcgct gtttggtttt gccgctgtgt
    10021 tgatgagtct gcgagcacgt aaacgttgat gtgcctgatg tatcctgcat gagcaaaacg
    10081 tactttctca ttgagctttg gggcatggtt atcatgcccc tttttcttac ggttcaggga
    10141 tacaagccac attgccatat tggggatgac gaataattgc ctgcggatca agcgagcgcg
    10201 tcctgtccat ttggatcaac ccgttcttcg ccatgacaaa tatttttcta ccatccgctg
    10261 aaaaacgaag gtgattaacg tagctggcag gataaacgcc aataatctgc ccggcattaa
    10321 tattgagcag gatgacataa ccttgcaggc catatgacac caacgctaaa tattgaccgt
    10381 ccgggctaat accctcaacg gaataaacgc tcctgctaca ccattgcgtc ataatgctgg
    10441 cttgttgatc ccctacgctt atacgtgaaa tcccctgact atcggcaaaa ataacctcgc
    10501 cgttttcagg tcgaaaacgc atcgccgata tcgttccacg cgcatcacct gcgggaaagt
    10561 cttctttctg atgcgtgatg gtattgatcc gcacgatgtt acctctgcct gctcccggaa
    10621 ccagtaatgt tctttcatcc ggggaaagtg ccataaccca ggcgcctggt ttaccgctcg
    10681 tccccgtccc gtcgtatttc tccagcggga tatccgccac atgctgataa gtcaccagat
    10741 caatcgctga cacactatta ctggtgcgat tcatcaccca ggcttttgtg ccatcacgcg
    10801 agaggataag gctgtcagga tgtgcaccgg tggtaatcga ctgaataatc tcccctgtcg
    10861 ccacgttaat gaccgataac gcattcgctt caaatgaagt cgcataaatc aacttttcat
    10921 ccgggcttaa tgccagcgat ctgacgccct gatctttaca gccaccacaa tgtttgactg
    10981 acttttcttt cgctatgggc gctgtaaagg tatgcaggat tttcttttgt ccgaggtcga
    11041 aaacaacaat gctgttcgtg ttttcaattg cggcatacaa catattacca tcccgactga
    11101 tcgccatatc atggatatcg gcaatctcag cgagcaaatc accactggcc tgttcacgac
    11161 ttaccggagc ggataatgtt tccctgaggg cgttgcgttc ctccgctaac tgctgctttt
    11221 tttgccgtcg ctcttgttca atctcctgtg gataaaacgc ttcacgcagg ctttgcctga
    11281 cctgtaaaac tgactcatcc atctgctgct gcgcaagcca gatagccccg gtcagaagaa
    11341 gactgcccgc aacaataatc ccgccttccg taacgagtcg gttttgccca gataaacgcg
    11401 cgctcagcca acccaacgcg ccacccgcaa tcaggccgcc aatgtggcaa gcgttatcta
    11461 ttcctgtttg ccgggcaccg tacaacagtg tcagcgccac catacccaac agaggaaata
    11521 ctcgccgctg gtttttatgg tatgtgcccg tacctgaagc aaactgtgtt gctatcgctg
    11581 caccagctat tcccagaatc gccccggatg ccccaacgct gatgactatc tgctcacttt
    11641 cgcgtaacgc ataacaggcg ctgatgagcc caccaaagac gccggagaat aaccaggtaa
    11701 tcaggagtcg ccatttcccc agaatctgtt ccgccacgcc tcccacgacc aggaatgcaa
    11761 acatattcat cagcagatga gaaacactac tgtgcagaaa gatactgctc aataagcgcc
    11821 agggttcacc cgacaaagta agaggcgcaa cgtttgcgcc gagatggata agtgcggatt
    11881 gatcgacctc ttcataactc atccattgtg cgagataaca aaatatccca atacagagtg
    11941 caaaaaggat aagggttatt gcgggtaatt gaacatttaa cggcttaacc gaagatgcag
    12001 acataaggtg tttcttcctt gaaaagacag gtaaaaagca tttttcccgt gtgcagttat
    12061 catcccggca aacgtcccat gccgaaaaga taattctgtc cgaaatatga ctttttcagc
    12121 ctaaaattac aatcgtaatt aattcgcccc taagtatttt ttggcatgta agttgcttta
    12181 tttactgtgt aagcgatgaa atcagccgcg acgcaaccag gttgcaccac cacaaatcag
    12241 cggtagcacc aaaaaggtgc ggtttaagaa ttttgcttcg gaatggtgcg gcgcatctcg
    12301 caaaaacaaa caataaaaca gcaatatctt tacatttagc caaactaaat gttacctaac
    12361 aggcacaaca ctgcacaata aagttgcaga cgataacaac acaaacactc acaacgggta
    12421 tccatgcgtt cttaacgcag aagataaagg agttggatat gcaattacgt aaacctgcca
    12481 cagcaatcct cgccctggcg ctttccgcag gactggcaca ggcagatgac gccgccccgg
    12541 cagcaggcag tacgctggac aaaatcgcca aaaacggtgt gattgtcgtc ggtcaccgtg
    12601 aatcttcagt acctttctct tattacgaca atcagcaaaa agtggtgggt tactcgcagg
    12661 attactccaa cgccattgtt gaagcagtga aaaagaaact caacaaaccg gacttgcagg
    12721 taaaactgat tccgattacc tcacaaaacc gtattccact gctgcaaaac ggcactttcg
    12781 attttgaatg tggttctacc accaacaacg tcgaacgcca aaaacaggcg gctttctctg
    12841 atactatttt cgtggtcggt acgcgcctgc tgaccaaaaa gggcggcgat atcaaagatt
    12901 ttgccgacct gaaagacaaa gccgtagtcg tcacttccgg cactacctct gaagttttgc
    12961 tcaacaaact gaatgaagag caaaaaatga atatgcgcat catcagcgcc aaagatcaca
    13021 gtgactcttt ccgcaccctg gaaagcggtc gtgccgttgc ctttatgatg gatgatgccc
    13081 tgctggccgg tgaacgtgcg aaagcgaaga aaccagacaa ctgggaaatc gtcggcaagc
    13141 cgcagtctca ggaagcctac ggttgcatgt tgcgtaaaga cgatccgcag ttcaaaaagc
    13201 tgatggatga caccaccgcg caggtgcaga cctccggtga agcggaaaaa tggtttgata
    13261 aatggttcaa aaatcccatt ccgccgaaaa acctgaacat gaatttcgaa ctgtcagacg
    13321 aaatgaaagc actgttcaaa gaaccgaatg acaaggcact gaactaatta caagaaccag
    13381 gggcggaaat tccagccctc tcgattgtta cgtagcacgg acagactata cgcctgatgg
    13441 tcgttcccca tcgggcctga aaaccgcaat acgctgggta acaatcttcg agggtagcag
    13501 ttaacgctgc taccctcttt tttctggagt agatttatgt ctatagactg gaactggggt
    13561 atttttttac aacaagcccc gttcggcaac accacctatc ttggttggat ctggagtggt
    13621 tttcaggtga cgatcgcttt gtcgatctgc gcctggatta tcgctttcct cgtcggttca
    13681 tttttcggca ttttacgtac cgttccaaac cgttttctct ccggtctggg tacgttgtat
    13741 gtcgaactgt tccgcaacgt gccgttgatt gtgcaattct ttacctggta tctggtgatc
    13801 ccggagctgt tgccggagaa aatcggcatg tggtttaagg ccgagctgga tcccaatatt
    13861 cagtttttcc tttcatccat gctctgcctg gggctgttta ccgccgcccg tgtttgcgaa
    13921 caggtacgcg ccgccattca gtcgttgccg cgtgggcaaa aaaatgccgc gctggcgatg
    13981 ggcctgacgc taccgcaggc ttatcgatac gtactgctgc ctaatgctta tcgcgttatc
    14041 gtcccgccga tgacttccga gatgatgaac ctggtgaaaa actccgccat cgcctcgact
    14101 attggtctgg tggatatggc ggcgcaggcg ggtaaattgc ttgattactc agcccacgcc
    14161 tgggagtcgt ttaccgctat tacgctggcg tatgtgttga ttaacgcggt tatcatgctg
    14221 gtaatgacgc tggttgaacg taaagttcgc ctgcctggca acatgggggg caaataatgt
    14281 acgagtttga ctggagttcc attgtccctt ccctgccata tctgctcgac gggctggtga
    14341 tcacgctgaa aatcaccgtc acggcagtcg tgattggtat tttgtggggc accatgctgg
    14401 cggtgatgcg cttgtccagc tttgcgcccg tagcgtggtt tgccaaagcc tacgttaacg
    14461 tattccgctc gattccttta gttatggttt tgctgtggtt ttacctgatc gtgccaggtt
    14521 ttctgcaaaa cgtgctggga ttatcaccaa aaaatgatat ccgtttaatt tcggcgatgg
    14581 tggcgttttc gatgtttgaa gcggcctact attcagagat tatccgtgct ggtattcaaa
    14641 gtatttctcg tggtcagtcg agcgccgcgc tggcgttggg aatgactcac tggcagtcga
    14701 tgaaactgat tattctgccg caggcgttcc gcgcgatggt gccgcttctg ctcactcagg
    14761 gcatcgtact gttccaggat acctcactgg tgtatgtgtt atctctggcc gatttcttcc
    14821 gtaccgcctc aaccattggt gagcgtgatg gtacgcaggt tgagatgatc ctgtttgccg
    14881 gatttgttta tttcgttatt agtcttagcg cgtcgttgtt ggtcagctac ttgaaaagaa
    14941 ggacagcata atgattaccc tgaaaaatgt ttcaaaatgg tatggtcact ttcaggtgct
    15001 gaccgactgc tcaaccgaag tgaaaaaagg cgaagtggtg gtggtttgcg gcccgtctgg
    15061 ttccggcaaa tcaacgctga ttaaaaccgt caacggcctc gaaccagtgc agcaaggtga
    15121 aatcaccgtc gatggtatcg tggttaatga caagaaaacc gatctggcaa agctgcgttc
    15181 ccgcgtcggg atggtattcc agcatttcga gctgttccct catctgtcga ttatcgaaaa
    15241 cctgaccctg gcgcaggtga aagtgcttaa acgcgataaa gcgcccgctc gtgaaaaagc
    15301 attgaaattg ctggagcgcg tgggactttc cgcacatgcc aataaatttc cggcgcagct
    15361 ttccggcggt cagcagcagc gtgtggcaat cgctcgcgca ttgtgtatgg atcctattgc
    15421 gatgctgttc gacgaaccga catcggcgct ggatccggag atgatcaacg aagtgctgga
    15481 cgtgatggtg gaactggcga acgaaggaat gaccatgatg gtggtgaccc acgaaatggg
    15541 ctttgcccgc aaagtggcga atcgggtgat ctttatggac gagggtaaaa ttgtcgaaga
    15601 ctcgccgaaa gacgctttct tcgatgatcc gaaatcggac cgcgcgaaag acttcctcgc
    15661 gaaaatcctg cattaatcac tctggcgcgc atacccaagt gcgcgccatc tcacgcttta
    15721 tcgtttcgcc cctcgtatct gttacatcct ggcgttaccc ttgtcgcaaa gaagcacaca
    15781 acaaggagca acaatggcac tgccaattct gttagattgc gacccaggtc atgacgacgc
    15841 tatcgcaata gttctcgccc tcgcctcacc agagcttgat gtcaaagcaa ttacgtcttc
    15901 cgccggaaac cagacaccag aaaaaacctt acgcaatgtt ctgcgtatgc tgaccttgct
    15961 taatcgcacc gatattccgg tagcaggcgg cgcggtaaaa ccgttaatgc gtgagttgat
    16021 tatcgcggac aatgtgcacg gcgaaagcgg tctggatggc ccggcattac cggaaccgac
    16081 gttcgcaccg caaaactgta cggcggtaga gctgatggcg aaaacgctac gcgaaagtgc
    16141 ggaacctgtc acgattgtgt ctaccggacc gcaaactaac gttgccttgc tgctcaatag
    16201 ccatccggaa ctgcatagca aaattgcccg tatcgtgatt atgggcggcg caatggggct
    16261 tggtaactgg acgcctgcgg ctgaatttaa tatttacgtt gacccggaag cggcagaaat
    16321 tgtcttccag tcagggatcc cagtggtgat ggccggtctg gatgttactc acaaagcaca
    16381 aatccacgtt gaagacaccg agcgtttccg cgcgattggc aaccctgttt caaccattgt
    16441 tgccgaactg ctggatttct tcctcgaata tcataaagac gaaaaatggg gctttgtcgg
    16501 tgcaccgctg catgacccat gcaccatcgc ctggctgttg aaacccgagt tgtttaccac
    16561 tgttgagcgc tgggttggcg tggaaacaca ggggaaatat agccagggta tgacggttgt
    16621 tgattattat tatctgactg gcaataaacc gaatgccact gtaatggtcg atgttgatcg
    16681 tcagggcttt gttgatttac tggctgatcg tctgaaattt tacgcttaac acacgtcatt
    16741 gattaagatc accacgcgtt acctgggtgg tcttaatctg tttatctgtt tatctctttt
    16801 tctttcaaga tataaatgca atggataatg cagaactcgc cattggtatc gatctcggca
    16861 ctaccaatag tttaattgcc gtctggaaag acggtgccgc gcaattaatt ccaaataagt
    16921 tcggtgaata tttaacacca tccataatta gcatggatga aaataatcat attttagtcg
    16981 gaaaaccggc tgtatcacgg cgtacttcgc atccggataa aacggcagcg ttatttaaac
    17041 gtgcaatggg cagtaatacc aactggcggt taggcagcga cacatttaac gcgccagaac
    17101 tgtcctcttt ggtattacgc tcattaaaag aagatgccga agaatttctg caacgtccga
    17161 ttaaagatgt ggtgatctcc gttccggctt atttcagcga tgaacaacgc aagcataccc
    17221 gtttagcagc ggagttagcc gggttaaatg cggtacgctt aattaatgaa cccacagcag
    17281 ctgcgatggc atatggcctg catacgcaac aaaatacccg ctcgctggtt tttgatctcg
    17341 gtggcggcac gtttgacgtc acggtgctcg aatacgccac gccggtgatt gaagttcacg
    17401 cctccgctgg cgataacttc ctcggtggcg aagattttac ccatatgttg gtcgatgacg
    17461 ttttaaagcg cgcggatgtc gccaggacca cgcttaacga gagtgaactg gcagccttgt
    17521 acgcctgtgt ggaagcggca aaatgtagca atcaatcgcc attgcacatt cgctggcagt
    17581 accaggatga aacgcgggag tgcgaatttt acgagaacga actggaagat ttgtggttgc
    17641 cgctgctcaa tcgcttgcga gtgccgattg aacaggcgtt gcgcgatgca cgtctgaagc
    17701 cgagtcaaat cgacagtctg gtgctggttg gcggcgcgtc acaaatgccg ctggtgcagc
    17761 gaatcgccgt gcgtctgttt ggcaaattac cgtatcaaag ttacgacccg agcaccattg
    17821 tcgcgctggg cgcagcaatc caggccgcct gccgcttacg cagtgaagat attgaagagg
    17881 tgatcctcac tgatatttgc ccttactcgt tgggcgttga agttaaccgc cagggcgttt
    17941 ccggcatttt ctcgccaatt attgaacgaa acaccacagt acccgtgtcg cgtgtagaaa
    18001 cttactcgac catgcacccg gaacaggatt ctattacggt taacgtctat cagggagaaa
    18061 accacaaagt taaaaataac attctggttg aatccttcga tgtgccgttg aagaaaaccg
    18121 gggcttatca gtcgattgat attcgcttta gttatgatat caacggcttg cttgaagttg
    18181 acgtgcttct ggaagatggc agcgttaagt ccagagtcat taatcatagc ccggtaacat
    18241 tgagcgcgca gcagattgaa gagagtcgga cgcggttatc cgcattgaaa atttatccgc
    18301 gcgatatgct aatcaaccgc acctttaagg ccaaactgga agagttgtgg gcgcgggcgc
    18361 tgggtgacga gcgagaagag attggccggg tgatcaccga ttttgatgcg gcgttgcagt
    18421 caaacgatat ggcccgcgtc gatgaagttc gtcgacgggc gagcgattat ttggctattg
    18481 agatccctta aacacggctg ccggatgcgg cataaacgct gcatccggca tcggcgcaaa
    18541 aaacacatcc tcagctaaac aacatcgcga tttttccgag cgcataacct atcatcagca
    18601 ccgttaagct gattttgata atactggtaa tgctctgtgg tttttcatcc tcatcatttt
    18661 tttcttcatg gttttcaggt gttggctcat cgacatcttc atcctccggc tcgagtttca
    18721 ctttcgtcaa cagcatctgg gtgataatgg cctctaatgg atgagcatct ccaccttcca
    18781 gccttgtatt aagacgggcc agcatctctt gtttcgggaa cgcttccagc acaacctgtt
    18841 ccgctacccg atacaaatcc agtaacggtg aatcttgctc tgtctcgcta ccgactctcg
    18901 atttccacca cgcccatgct ttgcgcttga agaataacga ctcaataaac gattgaccta
    18961 acggcgataa ttttccgcta tgtaaaatct ccgcccataa cagccgcgtc cgggcgctct
    19021 caccaggaac atatttcaac ggtttacctt gcgcatctac catgcttcgc gcttcgataa
    19081 ccatcactaa taacgcgaaa taatccgggc aatgacgctt gctccactgc aacaacaaat
    19141 tatcagcttg tgtagatggg taactttccc agtacgcgca gagatcggca agcaggcttt
    19201 ccccctcgcc gcagtacaaa cgttgtgcac actggtaata ccgggcatct tcgctttctg
    19261 gttcttccgc ttgccactgt ttggctacag gaatgagttc cgccatacct gtttgcaccg
    19321 agctgtacca gcgcaacagt ttgcgatgta actttgcatc atcaggaatg aaccacggtc
    19381 cttccatcgc caaaaacgca gtgacatagt caggatggta acggcagcaa cgttccagcg
    19441 cgaagtaata ttcaacagtc tggttctgta acgcgacggg atattccaga aggctaagga
    19501 aatcgaaaca atcaccagcc tgaatggcct caagaaaagc ctccctttct tcctcatcca
    19561 ttccttcgct gtcattaaac gaatgccagt tcaaacgctc tgccaacaaa gaagcacaat
    19621 ttaatgaaag atatcgcgct tctatagcga ttgcacacag cggccagcgt aattgatcga
    19681 catcctccat gttccaggta tttaattgct gaataaattt ctgccaggcg gagggctgaa
    19741 aacgatcact ttctgaatcc agtaatgtcc tgaatgcacg tagtatttca tgttcagcgg
    19801 tggcatcttt ttcttcatca tctgcttcct cgacaggatt taccgccagt cgcagcgcct
    19861 cttcataggc ctggcgtaat gctttaaacc cctgcggatc ggtttcggga tgacacaacg
    19921 gtaagcgagc aagataagcc tggcggataa tgtctatttg cgtcgtgctt tcaatttcga
    19981 ggatttgcca acatgttttc ataggtcgct cacatcctta agcgttaccg ggcattcagg
    20041 ctggacagtt aatggttgcc agggaagcgt ggcatagaaa cggtcgcttc ccgctgccag
    20101 ctcttcgaac atccgactcc cggcgctatc gtatttgtac agggttagcg cctcgcgtag
    20161 cgaagcgttg tcttcgcact gagaagattg ccagtagaga cgccctagtg aataggcaga
    20221 aaaatagtgc atccagctgt gatagtaatg gtgagctcgt gcatagacgc gcgattgcag
    20281 ccataaactc tcttcttccg tcagccagtt gttgagcaca ccgacccgtg acagaaaccc
    20341 cattcgaaca taatcccagg ctttgattcc cccttctcca cagcacatag cggtttgggc
    20401 aacgaattgg gtataaatca accctctctc cgagccacca gcaattaatg ctttccactc
    20461 ttcaggggaa gagcgttgcc atgtcagata taagccagct aaatcattgg catgaccatc
    20521 atcagtcatc tgataaatag tctgaattaa atcatcacga ttatttattc cccaggatga
    20581 tgagagatcg aattctttta tcccagtata catttttggc gagtcatgcg cgggtttgca
    20641 actctgatta agtatttcca tgggcgcaga aagcgcaaaa agaagatatt gttcttcctt
    20701 attcattttt taataatccc ttaagtgcat taactgacta cttttttatt attgatgagt
    20761 ataaacttga cgctttcaaa ataaaaagaa aatcgaagca ttcacacatg aataaaaaat
    20821 caatgtatat attcgcaatt tttatagttg cagcaattac ctgtattagc caacccaaga
    20881 aaacgacgtt gcgtgataaa gccatggtga attatgcctt tgattattta agatcaccag
    20941 gcagtctacc gttaaccacg gcagccacgg agctttccgc gattcatggt cactcaacat
    21001 cgcaatatcg ccttggcgaa ttttatcttc atggtagcga cggtaaacca ctggattata
    21061 cacaggcgag atactggtat gagcaatcag cagaacagga aaatccgcgc gcacaaagca
    21121 aactggggtg gatctacctg aaaggcctgg gagtcaaacc cgatacccgt aaagcaattc
    21181 tctggtataa ggaagcagct gaacaagggt atgctcatgc tcaatatact ttgggtttga
    21241 tctacagaaa tggcactggt attaatgtta accattatga atctcaaaaa tggttaaaac
    21301 tggccgccaa acaacattac aaaaatgcgg aaagattact tgccggactt cccgcacatt
    21361 aacgtcgact gtcacccgaa gagatggagt atttttccta tcaaaccggc aaagataaaa
    21421 atgtagaaaa taattttgat gacgcccaga cagcccagcc cgccatcgtc aacgggcatt
    21481 ggctgcggtt gcggctccgg ctcgacggtt tcctgaccgg taaaattcgc cgcctgaatt
    21541 aataatcgac gaataattaa tgcttccagt ggacaacgat cttctgcatc aaggcggctc
    21601 acaatcggca ttaatgattc ttctgtcggt tggctggcct gcactaattg tttggcaact
    21661 aaatataatg gccataaggg atgttctgta tcacgcgtga gtgagatccg cggcttacgt
    21721 cctttgtgca aaaccatctc gaccagtgaa cgccccagcg cagagagttt ttcatcgctg
    21781 agtatttcgt ataaatgaaa acgcgtacgg gcgctgtcgc cggggacata aagtagctgt
    21841 ttaccctgat cgttaaccag ttcacgagct tcaatcatca tcaccactaa cgggtaataa
    21901 tcgacccgat gctgacggca ccattgcaat attaaagcat ccgcctgggt ggagggatac
    21961 tcgcgccagt agtcacaaag ttctgccagc aagctttcgc cttctccaca atagacgcgt
    22021 tgcgcgtatt cataataacg cgcatcttca ttatcaggtt cagcttcccg ccacgaccgg
    22081 gcgtaatcca gaagttcggg gatatccagg cgacctgcgc tataccagcg gagtaaattt
    22141 ctgtgcagtt ttgcatcatc ggggatgatc actgcgcgat gttgcgctaa atacaatgtc
    22201 agccattctg gatgcgatga ccaaatccga gcgaacattt ggtaaaaatc gatggtgtca
    22261 ttttgcacgg caacgggcag atgcagaatg gtctggaagt taaaaacatt ccccttcgca
    22321 atggcatata aaaaggattc cagttcttct tcgtctattt ccccgacgtt ttcctcctgc
    22381 aaccaccgca atctttctgc taataacacc acgcactcga aggataaatg ggcagtgttc
    22441 atggcgattg tgcacagcga ccagcgtaat tcatcaatct cttccatcga gcaataattt
    22501 aattgctgaa tgaatcgctg ccaggcggag ggcagaaaac gttcgctatc agaggcaagt
    22561 aacgcgcgaa aggcgagcag aatttcatgt tctgctacct catattcttc tggttgccaa
    22621 acagatttag caggcgactg cgcaatccgg agcgcctcct catacgcctg acgaagttgt
    22681 ttaaaaccct gcggatcggt ttctggatga aaggacggta acagcgccag ataagcgcgg
    22741 cggataatat cgacgtcagt cgtttcctct atttcgagga tcttccagca atttttcata
    22801 ggtcgctcat atccttaagc gtttccgggc atgtggggta gtcagccagt ggttgccagg
    22861 acaaggcagg gtaatacgca tcatcactgg caaataattg ataaaacata tcattgccgg
    22921 aatcgtcgta ttctttacga gctaagaatt cgcgtaataa aggcagatga tcatcttcgg
    22981 gagactgcca atattgccga ccaaaggtat aaccggcaaa gtattgccgc cagttactat
    23041 aataacgtag agcccgtaga tgaatacgcg actggatcca tagactttct tcttcactca
    23101 accagttatt aagtacgccc attcgactta aaaaacccat ccggacataa tcccatgctt
    23161 taattcctcc ctcaccacag cattcggctg tgctggcgac aaattgcgca taagcctgac
    23221 cttgctcatt tagttcagca agatagtcac gccattcaca tggcgaataa cgaaaccagc
    23281 ggtgataaaa ccctgccaac cgagcggcat gaccgttatc gatcattcgg tgaattgtgc
    23341 gcagcaggtc atcacggtca tcaattcccc atgaggaaga aaggtcaaaa aagcatgtac
    23401 ctaaatacat tttgggggat gaatgcgaag gtaaacagca ttcattgcgt atttccatcg
    23461 gagtggaaag tgcaaacaat attttttgcg accccatgtc catataattt atccttaaac
    23521 attcatttaa tgaatgtaaa ctacatttcg agactcagtt atttatactt ttctgacgca
    23581 acataatacg cataaaataa ggcttacgca aaaatggaac acataaaaat ataatatgtt
    23641 ttatctacaa ccataaggaa atgataatgg tttttacgtc aagttgctgc gataatttat
    23701 caatagatga gattatcgaa cgtgctgaaa aaggcgattg cgaggctcaa tatattgttg
    23761 ggttttatta taatcgcgat agcgcaattg actctccgga cgacgaaaag gccttttact
    23821 ggctgaagct ggccgctgag caaggtcatt gtgaagcaca gtattcctta gggcaaaagt
    23881 ataccgagga taaaagccgt cataaagata atgagcaagc catcttctgg ctgaaaaaag
    23941 ctgccctaca aggccatact ttcgcttcca acgcccttgg ctggacactg gatcgtggag
    24001 aagcccccaa ttataaagaa gcggttgtct ggtatcagat agccgcggag agcggaatgt
    24061 cttatgcgca aaataatctt gggtggatgt acagaaatgg caacggagtc gcaaaagact
    24121 atgcgctggc atttttctgg tacaaacaag ctgcattaca aggccatagt gacgcgcaaa
    24181 acaatctggc cgatctttat gaagacggaa aaggcgttgc tcaaaacaag acactcgccg
    24241 cattctggta tttgaaaagc gcacagcagg gtaatcggca cgcccagttt caaattgcat
    24301 gggattataa cgctggcgaa ggggtggacc aggactataa gcaagcgatg tactggtatc
    24361 tgaaggctgc cgctcaggaa agcgtcggcg cttacgtcaa catcggttat atgtataaac
    24421 acggacaagg cgttgagaaa gattatcagg ctgcctttga atggtttacg aaagccgctg
    24481 aatgcaatga cgccactgcc tggtataacc tggccattat gtatcactac ggagaaggaa
    24541 gacctgtcga tctccgacag gctctcgacc tgtatcgtaa agttcagtca tccggaacca
    24601 gagatgtcag tcaggaaatt cgtgagactg aagatttact gtagagataa aaacaagggc
    24661 cggggaattt cattcggccc atattctctt tattgctgtg cgaaatcaga ctattacggc
    24721 tcaaacgggc gtctctggaa ctggtcatga ccacatttcg ggcatagcgt cagcacttcc
    24781 ggtgtgtaga tcgggagatg gaagtgacat ttctcgcaga ccagatttcc cagcccgacc
    24841 acttctccgc tgtgataaac cccatgatga ttgaggtcct ggaaaacttc gcgccattca
    24901 agctgcgttt tatcggtgat atctgccagc tcctgccata agctttcttt aatcacccgc
    24961 ataaagacgc tgtcagattc ttctttcagg ctctcttcat agctcatggc gaactcttcc
    25021 aggtcacgtc ttacagctcg cgtcagctca tcgacctcgg ttcgcgttaa ctcccctgtt
    25081 tttattacgc gctcgcgcgc ctgttccacc agtgcgtcga tatcacgttc gccattgcgc
    25141 aggcgttcgc tcagtgacgc gaccagttca cggtaatatt gagcaacctt gttcatcgtt
    25201 tcatctcccg ggtcgttaac tgactataat aatagacgtt ctttgttcct tgctttgcta
    25261 atacgcccac aaactctgta aattaatgta tgaataatcc gggcatcatt tcaacgtcga
    25321 gtgcgcgaaa ggctgttttg acgcggacgt ttgggctatg ctatgcggat ctgaaaaacc
    25381 acctcaacgc tacatttgta gccgtattga aaacaggacc actggctgcc atgcaagagc
    25441 aataccgccc ggaagagata gaatccaaag tacagcttca ttgggatgag aagcgcacat
    25501 ttgaagtaac cgaagacgag agcaaagaga agtattactg cctgtctatg cttccctatc
    25561 cttctggtcg actacacatg ggccacgtac gtaactacac catcggtgac gtgatcgccc
    25621 gctaccagcg tatgctgggc aaaaacgtcc tgcagccgat cggctgggac gcgtttggtc
    25681 tgcctgcgga aggcgcggcg gtgaaaaaca acaccgctcc ggcaccgtgg acgtacgaca
    25741 acatcgcgta tatgaaaaac cagctcaaaa tgctgggctt tggttatgac tggagccgcg
    25801 agctggcaac ctgtacgccg gaatactacc gttgggaaca gaaattcttc accgagctgt
    25861 ataaaaaagg cctggtatat aagaagactt ctgcggtcaa ctggtgcccg aacgaccaga
    25921 ccgtactggc gaacgaacaa gttatcgacg gctgctgctg gcgctgcgat accaaagttg
    25981 aacgtaaaga gatcccgcag tggtttatca aaatcactgc ttacgctgac gagctgctca
    26041 acgatctgga taaactggat cactggccag acaccgttaa aaccatgcag cgtaactgga
    26101 tcggtcgttc cgaaggcgtg gagatcacct tcaacgttaa cgactatgac aacatgctga
    26161 ccgtttacac tacccgcccg gacaccttta tgggttgtac ctacctggcg gtagctgcgg
    26221 gtcatccgct ggcgcagaaa gcggcggaaa ataatcctga actggcggcc tttattgacg
    26281 aatgccgtaa caccaaagtt gccgaagctg aaatggcgac gatggagaaa aaaggcgtcg
    26341 atactggctt taaagctgtt cacccattaa cgggcgaaga aattcccgtt tgggcagcaa
    26401 acttcgtatt gatggagtac ggcacgggcg cagttatggc ggttccgggt cacgaccagc
    26461 gcgactacga gtttgcctct aaatacggcc tgaacatcaa accagttatc ctggctgctg
    26521 acggctctga gccagatctt tctcagcaag ccctgactga aaaaggcgtg ctgttcaact
    26581 ctggcgaatt caacggtctt gaccatgaag cggccttcaa cgccatcgcc gataaactga
    26641 ctgcgatggg cgttggcgag cgtaaagtga actaccgcct gcgcgactgg ggtgtttctc
    26701 gtcagcgtta ctggggcgcg ccgattccga tggtgacgct ggaagacggt accgtaatgc
    26761 cgaccccgga cgaccagctg ccggtgatcc tgccggaaga tgtggtgatg gacggcatta
    26821 ccagcccgat taaagcagat ccggagtggg caaaaactac cgttaacggt atgccagcgc
    26881 tgcgtgaaac cgacactttc gacaccttta tggagtcctc ctggtactat gcgcgctaca
    26941 cttgcccaga gtacaaagaa ggtatgctgg attccgaagc ggctaactac tggctgccgg
    27001 tggatatcta cattggtggt atcgaacacg ccattatgca cctgctctac ttccgcttct
    27061 tccacaaact gatgcgtgat gcaggcatgg tgaactctga cgaaccagcg aaacagttgc
    27121 tgtgtcaggg tatggtgctg gcagatgcct tctactatgt tggcgaaaac ggcgaacgta
    27181 actgggtttc cccggttgat gctatcgttg aacgtgacga gaaaggccgt atcgtgaaag
    27241 cgaaagatgc ggcaggccat gaactggttt ataccggcat gagcaaaatg tccaagtcga
    27301 agaacaacgg tatcgacccg caggtgatgg ttgaacgtta cggcgcggac accgttcgtc
    27361 tgtttatgat gtttgcttct ccggctgata tgactctcga atggcaggaa tccggtgtgg
    27421 aaggggctaa ccgcttcctg aaacgtgtct ggaaactggt ttacgagcac acagcaaaag
    27481 gtgatgttgc ggcactgaac gttgatgcgc tgactgaaga tcagaaagcg ctgcgtcgcg
    27541 atgtgcataa aacgatcgct aaagtgaccg atgatatcgg ccgtcgtcag accttcaaca
    27601 ccgcaattgc ggcgattatg gagctgatga acaaactggc gaaagcacca accgatggcg
    27661 agcaggatcg cgctctgatg caggaagcgc tgctggccgt tgtccgtatg cttaacccgt
    27721 tcaccccgca catctgcttc acgctgtggc aggaactgaa aggcgaaggc gatatcgaca
    27781 acgcgccgtg gccggttgct gacgaaaaag cgatggtgga agactccacg ctggtcgtgg
    27841 tgcaggttaa cggtaaagtc cgtgccaaaa tcaccgttct ggtggacgca acggaagaac
    27901 aggttcgcga acgtgctggc caggaacatc tggtagcaaa atatcttgat ggcgttaccg
    27961 tgcgtaaagt gatttacgta ccaggtaaac tcctcaatct ggtcgttggc taagcgcggg
    28021 aggaagcgtg cgatatctgg caacattgtt gttatctctg gcggtgttaa tcaccgccgg
    28081 gtgtggctgg catctgcgtg ataccacgca ggttccttcc actatgaagg tcatgatcct
    28141 ggactcaggc gatccgaacg ggccattaag ccgtgcagtg cgtaaccagt tacgtctgaa
    28201 tggtgtcgag ttgcttgata aagaaaccac gcgtaaggac gttccatccc tgcgtttggg
    28261 taaagtgagc atcgcgaaag ataccgcatc ggtattccgt aacggtcaaa cagcagagta
    28321 tcagatgatc atgacggtga atgcgaccgt gttgatcccc ggccgtgata tctacccgat
    28381 tagcgccaaa gtcttccgtt cgttctttga taacccgcaa atggcgttag cgaaagataa
    28441 cgaacaagac atgatcgtaa aagagatgta cgaccgtgct gccgaacagc tgattcgtaa
    28501 gctgccaagc atccgtgctg cggatattcg ttccgacgaa gaacagacgt cgaccacaac
    28561 ggatactccg gcaacgcctg cacgcgtctc caccacgctg ggtaactgat gattcggttg
    28621 tacccggaac aactccgcgc gcagctcaat gaagggctgc gcgcggcgta tcttttactt
    28681 ggtaacgatc ctctgttatt gcaggaaagc caggacgctg ttcgtcaggt agctgcggca
    28741 caaggattcg aagaacacca cactttttcc attgttccca acactgactg gaatgcgatc
    28801 ttttcgttat gccaggctat gagtctgttt gccagtcgac aaacgctatt gctgttgtta
    28861 ccagaaaacg gaccgaatgc ggcgatcaat gaacaacttc tcacactcac cggacttctg
    28921 catgacgacc tgctgttgat cgtccgcggt aataaattaa gcaaagcgca agaaaatgcc
    28981 gcctggttta ctgctcttgc gaatcgcagc gtgcaggtga cctgtcagac accggagcag
    29041 gctcagcttc cccgctgggt tgctgcgcgc gcaaaacagc tcaacttaga actggatgac
    29101 gcggcaaatc aggtgctctg ctactgttat gaaggtaacc tgctggcgct ggctcaggca
    29161 ctggagcgtt tatcgctgct ctggccagac ggcaaattga cattaccgcg cgttgaacag
    29221 gcggtgaatg atgccgcgca tttcacccct tttcattggg ttgatgcttt gttgatggga
    29281 aaaagtaagc gcgcattgca tattcttcag caactgcgtc tggaaggcag cgaaccggtt
    29341 attttgttgc gcacattaca acgtgaactg ttgttactgg ttaacctgaa acgccagtct
    29401 gcccatacgc cactgcgtgc gttgtttgat aagcatcggg tatggcagaa ccgccggggc
    29461 atgatgggcg aggcgttaaa tcgcttaagt cagacgcagt tacgtcaggc cgtgcaactc
    29521 ctgacacgaa cggaactcac cctcaaacaa gattacggtc agtcagtgtg ggcagagctg
    29581 aaagggttat ctcttctgtt gtgccataaa cccctggcgg acgtatttat cgacggttga
    29641 tatgaaatct ttacaggctc tgtttggcgg cacctttgat ccggtgcact atggtcatct
    29701 aaaacccgtg gaaacgctgg cgaatttgat tggtctgacg cgggtcacaa tcatccctaa
    29761 taatgttcct ccgcatcgtc cccagccgga agcgaacagc gtgcagcgta aacacatgct
    29821 tgaactggcg attgccgaca agccattatt tactcttgat gaacgcgagc taaagcgcaa
    29881 tgccccctct tacactgcgc aaacactgaa agagtggcgg caggaacaag gaccggacgt
    29941 gccgctggcg tttattattg gtcaggattc actgctgacc tttccgacct ggtacgaata
    30001 cgaaacgata ctcgacaatg cacatttgat cgtctgtcgg cgtccaggtt acccacttga
    30061 aatggcgcaa ccgcaatacc agcaatggct ggaagatcat ttgacacata acccggaaga
    30121 tcttcacctt cagcctgccg gtaaaattta tctggctgaa acgccgtggt ttaacatctc
    30181 ggcgaccatc atccgcgaac gtttgcaaaa cggtgaatcg tgtgaggatt tattgccgga
    30241 accggtactg acttacatta accaacaagg cttgtatcgc tgatacctgt cgttacggag
    30301 ggcatgatgc gactgtggtt aattcgtcat ggtgaaacgc aagcgaatat cgatggtctt
    30361 tacagcggtc atgcgcccac ccccctgacc gcgcgcggta ttgagcaagc gcaaaatctg
    30421 catacgctgc tacatggtgt ttcctttgat ctggttttat gcagtgaact ggaacgggca
    30481 cagcataccg cacgactggt tctcagtgac cgccagctcc ccgtgcaaat catccctgaa
    30541 ctcaacgaaa tgttttttgg cgactgggag atgcgacatc atcgcgacct catgcaagaa
    30601 gatgccgaaa actatagcgc gtggtgcaat gactggcagc atgcaatccc cacgaacggt
    30661 gaaggatttc aggcattttc gcaacgtgtg gaacgcttta tcgcaaggct tagtgaattt
    30721 cagcactatc agaatatttt agtcgtcagc catcagggtg tactgagtct gttaatcgcc
    30781 cgtttaattg gcatgcctgc cgaggctatg tggcattttc gcgttgacta ggggtgttgg
    30841 agcgccattg atatcaacca aaaattcgcg acgctacgcg tcctcaatag ccgtgccatc
    30901 ggggtcgaga atgcatgact tttctgtttt tttacgggta agccgcaacg accattgaca
    30961 gacccgggca ggctgatatt ctccgcagcc agactttttt cgccagacac gactttgtag
    31021 aaattgtttt acaaaaatgg cgatgcaatc tgcggcgcgg ggtgggatga tagcccactt
    31081 tcgaaagccg attcggcgac aattgtcccg aaatcgcctc tggttcaggt atactgacag
    31141 accattttta tctatttgat tcacccaggg ggaaaacttg cagggtaaag cactccagga
    31201 ttttgttatc gacaaaattg atgacctcaa aggtcaggac atcatcgcct tagacgttca
    31261 aggcaaatcc agcatcaccg actgcatgat catctgtacg ggtacgtcca gccgtcatgt
    31321 tatgtccatt gctgaccacg ttgtgcagga gtctcgcgca gcgggcctgt taccgctcgg
    31381 cgtagaaggt gaaaacagcg ccgactggat tgtcgtggat ttgggcgatg tgattgtcca
    31441 tgtcatgcag gaagagagcc gtcgcctgta tgaactggaa aaactctgga gttaatgcgt
    31501 gaagctgcaa cttgtcgccg tgggaacgaa aatgccggac tgggtacaaa ccggttttac
    31561 cgagtacctg cgtcgttttc cgaaagatat gcccttcgag ctgattgaaa ttccggccgg
    31621 aaaacgcggc aagaatgcgg acatcaagcg catactcgac aaagagggtg agcagatgtt
    31681 ggcggccgca ggcaaaaacc gcattgtcac cctcgatatt ccaggcaagc cctgggatac
    31741 gccgcagtta gccgctgagc tggaacgctg gaagctggat ggtcgcgacg tcagtctact
    31801 gattggcggg cctgaagggt tgtcgcctgc ctgtaaagcg gcggcggagc agagctggtc
    31861 gctgtcggcg cttaccctcc cccatccgct ggttcgcgtg ctggtcgcag agagtctgta
    31921 ccgggcgtgg agcatcacca ccaaccatcc ttatcaccgt gagtgataag ggagctttga
    31981 gtagaaaacg cagcggatga aactacagaa ctcttttcgc gactatacgg cagagtccgc
    32041 gctgtttgtg cgccgggcgc tggtcgcctt tttggggatt ttgctgctga ccggcgtgct
    32101 tatcgccaac ctgtataatc tgcaaattgt tcgctttacc gactaccaga ctcgctctaa
    32161 tgaaaaccgc attaagctgg tgcctatcgc gcccagccgc ggcattatct acgaccgtaa
    32221 tggtatccct ctggccctca accgcactat ctaccagata gaaatgatgc cagagaaagt
    32281 cgataacgtg cagcaaacgc tggacgcttt gcgcagcgtg gttgatctga ccgatgacga
    32341 tattgctgca ttccgaaaag agcgcgcacg ttcacaccgt ttcacctcta ttccggtgaa
    32401 aactaacctg accgaagtac aagtagctcg ctttgccgtc aatcagtacc gttttccggg
    32461 tgtcgaagtt aaaggctata aacgtcgtta ctatccttac ggttcggcgt tgacccacgt
    32521 catcggctat gtgtcgaaaa tcaacgataa agacgtcgaa cgcctgaata atgacggcaa
    32581 actggccaac tatgcggcaa cgcatgatat cggtaagctg ggcattgagc gttactatga
    32641 agacgtgctg cacggtcaga ccggttatga agaggttgaa gttaacaacc gtgggcgtgt
    32701 tatccgccag ttaaaagaag taccaccgca agccggacac gatatttacc tgacgctgga
    32761 tctcaaactc cagcaatata ttgaaacgct gctggcgggt agccgcgcag ctgtggttgt
    32821 caccgatccg cgtacaggtg gggtgctggc gctggtttcc acgcctagtt atgacccaaa
    32881 cctgtttgtt gacggtatct ccagcaaaga ttattccgcc ttgttgaacg ccccgaatac
    32941 accgctggtg aaccgcgcca cacagggggt ttatcctccc gcgtctacag ttaaacccta
    33001 tgtggcggtt tcggcattga gcgccggggt gatcacgcgc aatacgacgc tgtttgaccc
    33061 aggctggtgg caactgccag gttcggaaaa acgttatcgt gactggaaaa aatggggcca
    33121 cgggcgtctg aatgtcacaa gatcgctgga agaatctgcg gataccttct tctatcaggt
    33181 ggcctacgat atggggatcg atcgcctctc cgaatggatg ggtaaattcg gttatggtca
    33241 ttacaccggt atcgacctgg cggaagaacg ttccggcaac atgcctaccc gcgaatggaa
    33301 acagaaacgc tttaaaaaac cgtggtatca gggtgacacc attccggttg gtatcggtca
    33361 gggttactgg acagcgaccc caatccagat gagtaaggca ctgatgatcc tgattaatga
    33421 cggtatcgtg aaggttcctc atttgctgat gagcaccgcc gaagacggca aacaggtgcc
    33481 atgggtacag ccgcatgaac cgcccgtcgg cgatattcat tccggttact gggagctggc
    33541 gaaagacggt atgtacggtg ttgctaaccg ccctaacggt acggcgcata aatactttgc
    33601 cagcgcaccg tacaaaattg cggcgaaatc cggtaccgct caggtcttcg gtctgaaagc
    33661 gaacgaaacc tataatgcgc acaaaattgc cgagcgttta cgtgaccaca aactgatgac
    33721 cgcctttgcg ccatacaaca atccgcaagt ggctgtcgcc atgattctgg agaacggtgg
    33781 tgcgggtccg gcggttggta cactgatgcg ccagatcctc gaccacatta tgctgggtga
    33841 taacaacacc gatctgcctg cggaaaatcc agcggttgcc gcagcggagg accattaatc
    33901 atgacggata atccgaataa aaaaacattc tgggataaag tccatctcga tcccacaatg
    33961 ctgctgatct tactggcatt gctggtttac agcgccctgg ttatctggag cgccagcggt
    34021 caggatattg gcatgatgga gcgtaaaatc ggccaaatcg cgatgggtct agtcatcatg
    34081 gtggtgatgg cgcaaattcc tccacgcgtt tatgaaggct gggcccccta tctctatatc
    34141 atctgtatta ttttgctggt ggcggtagat gctttcggtg ccatctctaa aggtgctcaa
    34201 cgctggctgg acctcggtat tgttcgtttt cagccgtcgg aaattgccaa aatagccgta
    34261 ccactgatgg ttgcgcgctt tatcaaccgc gacgtttgcc cgccatcgtt gaagaacact
    34321 ggcatcgcgc tggtgctgat atttatgccc acgctgctgg tggctgcaca gcctgacctg
    34381 ggaacatcaa tcctcgttgc gctttccggt ctgtttgtac tgttcctctc tggccttagc
    34441 tggcgtctga ttggcgtcgc agtagtgctg gtggcggcgt tcattccgat tctgtggttc
    34501 ttcctgatgc atgattacca gcgccagcgc gtaatgatgc tcctagaccc ggaatcagac
    34561 ccactcggcg cgggctatca cattattcag tctaaaattg ctattggctc cggcggatta
    34621 cgcggcaaag gctggctgca cggcactcag tcacagcttg aatttctccc cgaacgccat
    34681 accgacttta tcttcgcggt actggcggaa gagctgggat tagtgggcat tctgattctg
    34741 ctcgctctct acattctgct gatcatgcgc gggctgtgga tagccgccag agcgcaaacc
    34801 acctttggtc gcgtcatggc tggtggctta atgctgatat tattcgttta tgtcttcgta
    34861 aatattggta tggtaagcgg tattctgccg gttgtagggg ttccgctccc actggtcagt
    34921 tatggaggat cggcgctaat tgtgctgatg gctgggttcg ggattgtaat gtcaatccac
    34981 acccacagga aaatgttgtc gaaaagcgtg taagaggtgc gcaatgcgta agcagtggct
    35041 cgggatctgc atcgcggcag gaatgctcgc ggcatgtaca agcgatgatg gtcagcaaca
    35101 gacggtaagt gtaccgcagc ctgcggtatg taacggcccc atagttgaaa ttagcggggc
    35161 ggacccgcgt ttcgaaccac tgaacgcgac ggcaaatcag gattaccagc gcgacggtaa
    35221 aagctacaaa atcgtgcagg atccgtctcg atttagccag gcgggactgg cggcaatcta
    35281 tgatgccgaa cccggcagta acctgacggc ctctggcgaa gctttcgatc cgacacagct
    35341 gacggcggcc catccaacgc ttccgatccc cagctacgcc agaatcacta acctggctaa
    35401 cgggcgaatg atcgtggtgc gcattaatga tcgcggccct tacggcaacg accgcgttat
    35461 ttcactttct cgcgcggcag ctgaccgtct taacacgtca aacaacacca aagttcgtat
    35521 cgatccgatt attgtcgccc aggatggttc gctttctggt cctggtatgg cttgtaccac
    35581 agtcgccaaa cagacttacg ccctgccagc acctcccgat ttaagcggtg gcgcgggaac
    35641 aagttcagtg tctggcccgc agggtgacat tcttccggtc agtaattcga cgctaaaaag
    35701 cgaagatccg accggcgcgc cggtaaccag tagcggtttc ctcggcgctc caacgacctt
    35761 agcgcctggt gtactggaag gcagcgaacc gacgcctgcg ccacagcccg ttgttacagc
    35821 tccgtcgaca acgcctgcaa cctcgcctgc aatggtgaca ccgcaagccg cctcgcaaag
    35881 cgccagcggc aactttatgg tgcaagtcgg ggccgtaagc gatcaggctc gtgcgcaaca
    35941 gtaccaacag caactgggac agaagttcgg cgtccccggt cgcgtaactc aaaatggcgc
    36001 ggtctggcgg atccagcttg gcccattcgc cagcaaagcc gaagccagta ccttgcagca
    36061 acgtttgcaa accgaagccc aattacagtc atttattact accgcgcagt agcgtaaagc
    36121 aggcatctga tgtgtcaatt tctgtaaatg acgttaacaa agtcacgcgg aaagtcagat
    36181 gcctgccggt agtgcatttg ctatagtagg gcactttttt aattccatca cggatgtcgt
    36241 agttcagacc atgaatacca ttttttccgc tcgtatcatg aagcgcctgg cgctcaccac
    36301 ggctctttgc acagccttta tctctgctgc acatgccgat gacctgaata tcaaaactat
    36361 gatcccgggt gtaccgcaga tcgatgcgga gtcctacatc ctgattgact ataactccgg
    36421 caaagtgctc gccgaacaga acgcagatgt ccgccgcgat cctgccagcc tgaccaaaat
    36481 gatgaccagt tacgttatcg gccaggcaat gaaagccggt aaatttaaag aaactgattt
    36541 agtcactatc ggcaacgacg catgggccac cggtaacccg gtgtttaaag gttcttcgct
    36601 gatgttcctc aaaccgggca tgcaggttcc ggtttctcag ctgatccgcg gtattaacct
    36661 gcaatcgggt aacgatgctt gtgtcgccat ggccgatttt gccgctggta gccaggacgc
    36721 ttttgttggc ttgatgaaca gctacgttaa cgcactgggc ctgaaaaata cccacttcca
    36781 gacggtacat ggtctggatg ctgatggtca gtacagctcc gcgcgagata tggcgctgat
    36841 cggccaggca ttgatccgtg acgtaccgaa tgaatactcg atctataaag aaaaagaatt
    36901 tacgtttaac ggtattcgcc agctgaaccg taacggcctg ttatgggata acagcctgaa
    36961 tgtcgacggc atcaaaaccg gacacactga caaagcaggt tacaaccttg ttgcttctgc
    37021 gactgaaggc cagatgcgct tgatttctgc ggtgatgggc ggacgtactt ttaaaggccg
    37081 tgaagccgaa agtaaaaaac tgctaacctg gggcttccgt ttcttcgaaa ccgttaaccc
    37141 actgaaagta ggtaaagagt tcgcctctga accggtttgg tttggtgatt ctgatcgcgc
    37201 ttcgttaggg gttgataaag acgtgtacct gaccattcca cgtggtcgca tgaaagatct
    37261 gaaagccagc tatgtgctga acagcagtga attgcatgcg ccgctgcaaa agaatcaggt
    37321 cgtcggaact atcaacttcc agcttgatgg caaaacgatc gagcaacgcc cgctggttgt
    37381 gttgcaagaa atcccggaag gtaacttctt cggcaaaatc attgattaca ttaaattaat
    37441 gttccatcac tggtttggtt aaaaattgaa cacttgaaag tgtaatttcc gtccccatat
    37501 actaagcatc agtaaaaaac tcccgccttc tggcgggagt tgctatttaa ttacgttacg
    37561 ccggagctga catgaaaacc aaacttaacg aactgcttga attccctact ccttttactt
    37621 acaaagttat ggggcaggcg ttacctgagc tggttgatca ggtggttgaa gtggtacagc
    37681 gccatgcgcc aggtgactac accccaacgg taaaaccaag cagcaaaggc aactaccact
    37741 cggtatctat cactatcaac gccactcata tcgagcaggt tgaaacactg tatgaagaac
    37801 taggcaaaat cgatattgtc cgcatggttc tgtaactcgc ttctccgtta cccggcccct
    37861 ggtcgggtaa ctcccctctc ctcgctgtga tatacttttc ccccactttt actcattctc
    37921 cacggagatg cagttttgta tcaggataaa attcttgtcc gccagctcgg tcttcagcct
    37981 tacgagccaa tctcccaggc tatgcatgaa ttcaccgata cccgcgatga tagtaccctt
    38041 gatgaaatct ggctggtcga gcactatccg gtattcaccc aaggtcaggc aggaaaagcg
    38101 gagcacattt taatgccggg tgatattccg gtgatccaga gcgatcgcgg tgggcaggtg
    38161 acttatcacg ggccggggca acaggtgatg tatgtgttgc ttaacctgaa acgccgtaaa
    38221 ctcggtgtgc gtgaactggt gaccttgctt gagcaaacag tggtgaatac cctggctgaa
    38281 ctgggtatag aagcgcatcc tcgggctgac gcgcctggtg tctatgtcgg ggaaaagaaa
    38341 atttgctcac tgggtttacg tattcgacgc ggttgttcat tccacggtct ggcattaaac
    38401 gtcaatatgg atctttcacc atttttacgt attaatcctt gtgggtatgc cggaatggaa
    38461 atggctaaaa tatcacaatg gaaacccgaa gcgacgacta ataatattgc accacgttta
    38521 ctggaaaata ttttagcgct actaaacaat cctgacttcg aatatattac cgcttaattg
    38581 catacctcaa tggcccaatt tacactgggt cattactcat aatacctgct aatgctttac
    38641 atctccttcg cgtttgtata ctttctttat cacttgcaga aatgcggtgc tacgccttgc
    38701 tccgtaactc accccattga ttaacctatt gaaatagttg attatggtga gcaaaagcgt
    38761 tccgttccaa ttagacataa gattattgta atttttttaa atattatttt tccatgaata
    38821 aaattggagt aagtgcgtgg atagtaataa tcaaattgaa ccctgtttaa gcagaaagtc
    38881 gtcagaaggt aaaccacaaa tatttacaac cttacgaaat attgatctta accttctgac
    38941 tatttttgaa gctgtatatg tacataaagg gatcgttaat gcagcgaaag tgcttaatct
    39001 gaccccctcg gcaatcagtc agtctattca gaaactgcgc gttatattcc ctgacccatt
    39061 gtttattcgc aaaggccagg gtgtcactcc taccgcattt gcgatgcatc tacatgagta
    39121 tatcagtcag ggccttgagt ccattcttgg cgcgctggat atcgaaggaa gctatgataa
    39181 gcaacgaacg ataactattg cgaccactcc ctcggtcgga gccctggtcc ttcctgtcat
    39241 ctatcgggcg attaaaactc actatccgca gcttttactg cgcaacccgc ccatcagcga
    39301 cgcggaaaac caactcagtc agtttcaaac cgatctcatc atcgataaca tgttttgcac
    39361 caatcgtacg gtgcaacatc atgttctgtt caccgacaat atggtgttaa tttgccgcga
    39421 gggaaatcca ctactctctt tagaagatga cagagagact atcgacaacg ctgcgcatgt
    39481 actcctgtta ccggaagggc aaaatttcag cggtctgcgg cagagagttc aagagatgtt
    39541 tccggaccgg caaatcaatt tcaccagcta caacattttg acaatcgctg cactggttgc
    39601 caacagtgac atgttagcga ttattcccag ccgtttttat aacctgttta gtcgttgctg
    39661 gccgctggaa aaactgcctt ttccgtcctt aaatgaggag caaatagatt tctctatcca
    39721 ctacaacaaa ttcagcctgc gtgatcccat cctgcacggg gtaatagatg ttatccgtaa
    39781 tgcattttga aaaagtattg ttacagccag atcacagcgc agaacgataa aatggcacaa
    39841 tccaacaaca attttacatt ttcgcgaccg ctttggctgc tttcaggtcc gtttcaatga
    39901 tatactgcca gtcgttaatt caaaaatagt tgataattac aacaatctat tgaattgaaa
    39961 cgctttcctt cgtaattcgc aactggaaca cgcacgctat gagtaaaccc attgtgatgg
    40021 aacgcggtgt taaataccgc gatgccgata agatggccct tatcccggtt aaaaacgtgg
    40081 caacagagcg cgaagccctg ctgcgcaagc cggaatggat gaaaatcaag cttccagcgg
    40141 actctacacg tatccagggc atcaaagccg caatgcgcaa aaatggcctg cattctgtct
    40201 gcgaggaagc ctcctgccct aacctggcgg aatgcttcaa ccacggcaca gcaacgttta
    40261 tgatcctcgg cgctatttgt acccgccgtt gtccgttctg tgatgttgcc cacggtcgcc
    40321 cggtagctcc tgatgccaat gaaccagtga aactggcgca gaccattgcc gatatggcgc
    40381 tgcgttatgt ggttatcacc tccgttgacc gtgatgacct gcgcgatggc ggtgcccagc
    40441 actttgcgga ttgcattact gccattcggg aaaaaagccc gcaaatcaaa attgaaactc
    40501 tggtgccgga tttccgtggt cgtatggatc gtgctctgga tattctgact gcaacgccac
    40561 cagatgtgtt caaccataac ctggaaaacg taccgcgtat ttaccgtcag gtacggcctg
    40621 gtgcagatta caactggtcg ctgaagctgc tggaacgctt taaagaagcg catccggaaa
    40681 tcccgaccaa gtctggtctg atggtgggac tgggtgaaac caatgaagaa attattgagg
    40741 taatgcgcga cctgcgccgt catggtgtga cgatgttaac gctggggcaa tatttgcagc
    40801 caagccgcca tcacctgccg gttcaacgtt acgttagccc ggatgagttc gacgaaatga
    40861 aagccgaagc gctggcgatg ggctttaccc atgctgcatg cggtccgttt gtccgctctt
    40921 cttaccacgc cgatttgcag gcgaaaggga tggaagttaa gtaatcctgt aataattact
    40981 tacatctgtc tgataaaaaa cccgcttttt g
//
LOCUS       DAESHI010000028        39969 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.028, whole genome shotgun sequence.
ACCESSION   DAESHI010000028 DAESHI010000000
VERSION     DAESHI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39969
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.028"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(42..117)
                     /locus_tag="KW011_001505"
     tRNA            complement(42..117)
                     /locus_tag="KW011_001505"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(82..84),aa:Lys,seq:ttt)
     gene            complement(151..226)
                     /locus_tag="KW011_001506"
     tRNA            complement(151..226)
                     /locus_tag="KW011_001506"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(191..193),aa:Lys,seq:ttt)
     gene            complement(275..350)
                     /locus_tag="KW011_001507"
     tRNA            complement(275..350)
                     /locus_tag="KW011_001507"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(315..317),aa:Lys,seq:ttt)
     gene            complement(354..429)
                     /locus_tag="KW011_001508"
     tRNA            complement(354..429)
                     /locus_tag="KW011_001508"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(394..396),aa:Val,seq:tac)
     gene            complement(579..654)
                     /locus_tag="KW011_001509"
     tRNA            complement(579..654)
                     /locus_tag="KW011_001509"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(619..621),aa:Lys,seq:ttt)
     gene            complement(819..1610)
                     /gene="cpoB"
                     /locus_tag="KW011_001510"
     CDS             complement(819..1610)
                     /gene="cpoB"
                     /locus_tag="KW011_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HBH7659874.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1620..2141)
                     /gene="pal"
                     /locus_tag="KW011_001511"
     CDS             complement(1620..2141)
                     /gene="pal"
                     /locus_tag="KW011_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HBH7659875.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(2176..3468)
                     /gene="tolB"
                     /locus_tag="KW011_001512"
     CDS             complement(2176..3468)
                     /gene="tolB"
                     /locus_tag="KW011_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HBH7659876.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3601..4833)
                     /gene="tolA"
                     /locus_tag="KW011_001513"
     CDS             complement(3601..4833)
                     /gene="tolA"
                     /locus_tag="KW011_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HBH7659877.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAADKKAAAAKAAAEKAAA
                     AKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIK
                     SAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKP
                     PSQAVYEVFKNAPLDFKP"
     gene            complement(4898..5326)
                     /gene="tolR"
                     /locus_tag="KW011_001514"
     CDS             complement(4898..5326)
                     /gene="tolR"
                     /locus_tag="KW011_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HBH7659878.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5330..6022)
                     /gene="tolQ"
                     /locus_tag="KW011_001515"
     CDS             complement(5330..6022)
                     /gene="tolQ"
                     /locus_tag="KW011_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HBH7659879.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(6019..6423)
                     /gene="ybgC"
                     /locus_tag="KW011_001516"
     CDS             complement(6019..6423)
                     /gene="ybgC"
                     /locus_tag="KW011_001516"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBH7659880.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6573..6866)
                     /gene="ybgE"
                     /locus_tag="KW011_001517"
     CDS             complement(6573..6866)
                     /gene="ybgE"
                     /locus_tag="KW011_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HBH7659881.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6866..6979)
                     /gene="cydX"
                     /locus_tag="KW011_001518"
     CDS             complement(6866..6979)
                     /gene="cydX"
                     /locus_tag="KW011_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HBH7659882.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6994..8133)
                     /gene="cydB"
                     /locus_tag="KW011_001519"
     CDS             complement(6994..8133)
                     /gene="cydB"
                     /locus_tag="KW011_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBH7659883.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(8149..9717)
                     /gene="cydA"
                     /locus_tag="KW011_001520"
     CDS             complement(8149..9717)
                     /gene="cydA"
                     /locus_tag="KW011_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HBH7659884.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(10565..13198)
                     /gene="mngB"
                     /locus_tag="KW011_001521"
     CDS             complement(10565..13198)
                     /gene="mngB"
                     /locus_tag="KW011_001521"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBH7659885.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLQDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YDMAVPLNLSERSARQSTGRVGGVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(13216..15132)
                     /gene="mngA"
                     /locus_tag="KW011_001522"
     CDS             complement(13216..15132)
                     /gene="mngA"
                     /locus_tag="KW011_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBH7659886.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            15301..16023
                     /gene="mngR"
                     /locus_tag="KW011_001523"
     CDS             15301..16023
                     /gene="mngR"
                     /locus_tag="KW011_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HBH7659887.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(16127..16996)
                     /gene="sucD"
                     /locus_tag="KW011_001524"
     CDS             complement(16127..16996)
                     /gene="sucD"
                     /locus_tag="KW011_001524"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HBH7659888.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16996..18162)
                     /gene="sucC"
                     /locus_tag="KW011_001525"
     CDS             complement(16996..18162)
                     /gene="sucC"
                     /locus_tag="KW011_001525"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HBH7659889.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(18256..19473)
                     /gene="odhB"
                     /locus_tag="KW011_001526"
     CDS             complement(18256..19473)
                     /gene="odhB"
                     /locus_tag="KW011_001526"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HBH7659890.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(19488..22289)
                     /gene="sucA"
                     /locus_tag="KW011_001527"
     CDS             complement(19488..22289)
                     /gene="sucA"
                     /locus_tag="KW011_001527"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HBH7659891.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(22590..23306)
                     /gene="sdhB"
                     /locus_tag="KW011_001528"
     CDS             complement(22590..23306)
                     /gene="sdhB"
                     /locus_tag="KW011_001528"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HBH7659892.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(23322..25088)
                     /gene="sdhA"
                     /locus_tag="KW011_001529"
     CDS             complement(23322..25088)
                     /gene="sdhA"
                     /locus_tag="KW011_001529"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HBH7659893.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(25088..25435)
                     /gene="sdhD"
                     /locus_tag="KW011_001530"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(25088..25435)
                     /gene="sdhD"
                     /locus_tag="KW011_001530"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HBH7659894.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(25429..25833)
                     /gene="sdhC"
                     /locus_tag="KW011_001531"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(25429..25833)
                     /gene="sdhC"
                     /locus_tag="KW011_001531"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBH7659895.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            26214..26321
                     /locus_tag="KW011_001532"
     CDS             26214..26321
                     /locus_tag="KW011_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659896.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            26527..27810
                     /gene="gltA"
                     /locus_tag="KW011_001533"
     CDS             26527..27810
                     /gene="gltA"
                     /locus_tag="KW011_001533"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HBH7659897.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            28200..28766
                     /locus_tag="KW011_001534"
     CDS             28200..28766
                     /locus_tag="KW011_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBH7659898.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28817..31275
                     /locus_tag="KW011_001535"
                     /pseudo
     CDS             28817..31275
                     /locus_tag="KW011_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            31290..32021
                     /locus_tag="KW011_001536"
     CDS             31290..32021
                     /locus_tag="KW011_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBH7659899.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            32018..33079
                     /locus_tag="KW011_001537"
     CDS             32018..33079
                     /locus_tag="KW011_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBH7659900.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVTPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            33231..34277
                     /locus_tag="KW011_001538"
     CDS             33231..34277
                     /locus_tag="KW011_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBH7659901.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(34274..35065)
                     /gene="nei"
                     /locus_tag="KW011_001539"
     CDS             complement(34274..35065)
                     /gene="nei"
                     /locus_tag="KW011_001539"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HBH7659902.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(35101..35835)
                     /gene="pxpA"
                     /locus_tag="KW011_001540"
     CDS             complement(35101..35835)
                     /gene="pxpA"
                     /locus_tag="KW011_001540"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HBH7659903.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(35825..36757)
                     /gene="pxpC"
                     /locus_tag="KW011_001541"
     CDS             complement(35825..36757)
                     /gene="pxpC"
                     /locus_tag="KW011_001541"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HBH7659904.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(36751..37407)
                     /gene="pxpB"
                     /locus_tag="KW011_001542"
     CDS             complement(36751..37407)
                     /gene="pxpB"
                     /locus_tag="KW011_001542"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBH7659905.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(37430..38173)
                     /gene="ybgI"
                     /locus_tag="KW011_001543"
     CDS             complement(37430..38173)
                     /gene="ybgI"
                     /locus_tag="KW011_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HBH7659906.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            38444..39925
                     /gene="dtpD"
                     /locus_tag="KW011_001544"
     CDS             38444..39925
                     /gene="dtpD"
                     /locus_tag="KW011_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HBH7659907.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         9674 a        10748 c         9735 g         9812 t
ORIGIN      
        1 ctcagatagc aaaaaagcgc ctttagggcg cttttttaca ttggtgggtc gtgcaggatt
       61 cgaacctgcg accaattgat taaaagtcaa ctgctctacc aactgagcta acgacccctt
      121 gcgggattat gctgctttaa tcattcagga tggtgggtcg tgcaggattc gaacctgcga
      181 ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccatac gggtgttacc
      241 tgaaagattt ctactcgggg ccaatcttta agattggtgg gtcgtgcagg attcgaacct
      301 gcgaccaatt gattaaaagt caactgctct accaactgag ctaacgaccc gagtggtggg
      361 tgatgacggg atcgaaccgc cgaccccctc cttgtaaggg aggtgctctc ccagctgagc
      421 taatcacccg atactgcgct ggatactaca aactacttcc accgaaccca ccgtaattag
      481 tggtgggtcg tgcaggatga ctcgcttcgc tcgccctacg ggccgttgct gacgcaacgt
      541 tatcctacac gcttaacatc tgtcttcgat gctaaaactg gtgggtcgtg caggattcga
      601 acctgcgacc aattgattaa aagtcaactg ctctaccaac tgagctaacg acccactttt
      661 tcgttgcttt cggtttgttt gatatcccgt ggcaacggcg gcatatatta ctgatttcag
      721 acttgagcgc aacaaaaatt tcgatgcaga tcactcaact gcttatgatt cgcacgacac
      781 gaccagaaat aatgcgactt ctggtcgtgt gttatgcatt acatcgcgtt cagacgtttt
      841 tgtgcctgtt tagcgccatc agtaccaggg tatttactga taacctgctg gtatacggct
      901 ttcgcttttg cggtgtcacc tttgtcctgc atgatgacgc cgactttaaa catcgcatca
      961 gcagcctttg gtgacttcgg atagtttttc actaccgaag caaaatagta cgccgcatca
     1021 tcttttttac ccttgttgta gtttaactga cccagccaat aattggcgtt tggcaggtaa
     1081 gttgaatcag ggtaattttt gatgaaattc tgaaatgcca ccattgcgtc atcctggcgg
     1141 gatttatcct gcaccagcgc aatagctgca ttgtaatccg tgtttgcatc gccgcttttc
     1201 accggcgcgc cagcattcgc agtaccagca tcagctgtcg gcgtcgttga tgccgccgca
     1261 ccgctttgat cgctgctggt tgattgcgcc gctgcaccac cgctgctgag gctgtcgatc
     1321 tgcaacagga tctgcttttg ccgctccacg acctgattca gttgatactg attttcctga
     1381 atctgaccac gcagggaatc aatatcggat tgattatcag aaagttgttg ctggagttgg
     1441 gttaaaagct ggctgtgagc attagaaata cgctcaagtt gagtgacgcg gtcttcgacc
     1501 gagcctgagc cgacactact gattggtgcc tgagcaaaag cggcccaggg ggccgctata
     1561 ccaaccagta acgacagact caatagttga tgtctgaagt tactgctcat gcaattctct
     1621 tagtaaacca gtaccgcacg acggtttttg gagtatgccg cttcgtcatg acccagtact
     1681 gcaggttttt ctttaccgta agaaacgatg gagatctggt ctgcagaaac gcctttaccc
     1741 tgcaggtaca tcttaacggc gttcgcacga cgttcaccca gggagatgtt gtattccgga
     1801 gtaccacgtt cgtccgcgtg accttctacg gtgactttgt aagacgggtt gctacgcagg
     1861 aagtttgcat gtgcatccag catttgagcg aagtcagaac ggatatcgta cttgtccaga
     1921 tcgaagtaaa cgatgttgtt ctgctgcagc tgttgcattt gcagacgagc ctgctcttcg
     1981 gaagacatgt tgccgttgcc gccgttcgca tccataccag tgccggcacc cagcatgcct
     2041 tcgctgccgt cattgctggc gttcttgttg gaagaacatg ccgcaattgc cataacaggc
     2101 agagcaatca tcagcccttt cagcactttg ttcagttgca tttcaatgat tcctttacta
     2161 ttcaattaat tattatcaca gatacggcga ccaggcaggg aatttgacct gtccatcagt
     2221 tgccggaaga cgcgctttga aacgcccatc tgtagaaacc aaattcagca cggatcccat
     2281 cccctgagaa gagctgtaga ttaccatagt gccgttaggt gccagacttg gcgtttcatc
     2341 caggaacgtg gacgacagaa cttgtacgcc tcccgttgcc agatcttgtt tggcaatgtg
     2401 ctgctgccca ccgttggagc tgaccattac cataaattta ccgtcgctgc tgacatccgc
     2461 atcctggttc tgcgaacctt cccaggtaat acgttgtggc gcaccgccgt tgatattcac
     2521 tttataaacc tgtggacgac cggcctggtc agaagtaaat gccaggttct ggctgtccgg
     2581 gaaccaggtc ggttcggtat tgttactgcg accatcagtc acctggcgga tctgaccaga
     2641 agccaaatcc attacgtaca ggttcaggct accggttttc gacaaggcga atgccagttt
     2701 gctgccgtct ggcgagaatg caggcgcacc gttgtgacgc gggaatgaag ccacctgacg
     2761 tacagcgccg tttgccagcg tttggataac tagcgcggaa cgaccgcttt cgaaggtcac
     2821 ataagccagt ttagaaccgt ctggtgacca cgccggggac atcagcggct gtggtgaacg
     2881 gtgaacgaca aactggttgt aaccgtcata gtcagatacg cgcagttcat acgggaactg
     2941 accgccgttg gtctgaacaa cgtaggcaat acgggtacgg aacgcacctt taatgccggt
     3001 cagtttttca aacacttcat cactggcggt atgaccagca tagcgcagcc actgcttgtt
     3061 cactttgtac gagttctgag caagtacagt acccggtgcg ccgccagtgt caacaagttg
     3121 ataagcaaca ttgtaagaac catccggatt cggagtgacc tgaccgacaa ctacagcgtc
     3181 aatacccagc gcggaccatg cagctggttg tacttcctgc gcactacccg gctgctgtgg
     3241 cagacgagcg cgatctaacg gattaaattt accgctgtta cgcaagtcgg cagcaacgat
     3301 gccgccaata tcttcaggtg ccgcaccagg gcccgcccac tggaaaggaa caacaccaat
     3361 aggacgaccg gaatctacac cgctgtcgat cacaatgcgg acttcagcat gcagaactga
     3421 tgcccacagt atgagaaaac caaatgctac tcgtaatgcc tgcttcatca tatctccctt
     3481 atctggaccg atggcccacg ataatttagc agaatgttaa caaactaaaa tacacaaaac
     3541 taccagaacc ccgtggcaac cggtgcctga tgttgaccgt cggaacagtc aacatcgcga
     3601 ttacggtttg aagtccaatg gcgcgttttt gaacacttca tatactgcct ggcttggtgg
     3661 tttcgggatc ttcgcaagtt tagctgctgc caacgcagcc tgacaaagtg cgggatcgcc
     3721 accttcaggt ttgatatcca gtaacatacc atcgggtgcc agttttatgc gcagcgtaca
     3781 ggttttgcct gcataggacg atgcgtcata gaacttactt tcgatagcag atttaatctg
     3841 cccggcatag ttattgatat cggcccctga tgcgccattg tttttagtat taccactccc
     3901 ggcaggcgaa gcattgttcc ctttcgcccc tccccccgtt ttcggtgcat tcttaccaga
     3961 gcttagctca ccgaaaatat catctgcctc tgcggcagct tttgctgcag cggctttttc
     4021 agctgctgct tttgccgctg ccgctttttt gtctgcggcg gctttttctg ccgctgcttt
     4081 tgccgctgcc gcttttttgt ctgcggcggc tttttctgcc gctgctttct tatcagctgc
     4141 tgccttatca gcagccgctt tttctgctgc ttttttgtcg gctgcagctt tctctgctgc
     4201 cgctttcttt cttgcttcag ctgcagcagc ttctgccgct tccgctttct tcttcagtgc
     4261 cgcagcggct gcctcggctt ttttctgcgc ttcggctgcg gctttggcgg cttctgcttc
     4321 tgcttttttc ttcgcgtctg ccgccgcttt cttcgctgct tcttccgcag ctttagcatc
     4381 cgcttcggcc ttcgctttag catctgccgc cgctttcgct gccgcctctt ccgcttgctt
     4441 ctgctttaac tcggcctgtt ttgcggcttc ttcagcctgc tttttctgtt cctgagcagc
     4501 taaccgctct ttctcaagtt gcttcaggcg ttcctgttca gccgcttgtt tctcacgcag
     4561 ttcttcagca gcctgctgtt cctttatctt gcgctgctca tcagaacgct tcgcgcttga
     4621 ttcctggctt tgcatgcgtt tgtactgctc aactaccgca cctgaatcaa ccatgacagc
     4681 gtcgatggac gaaccaccgc cgcctccggc tgaagcttct atattctcat cgaacgaact
     4741 ccagagcagc gccgcaaata agatgacatg cagcactgct gaaataatta tcgcccgctt
     4801 gagcttgtcg ttttgttcgg ttgcctttga cactctcggt ttccaaaaac tgttcgcctg
     4861 ttacccgctc tctttcaagc aagggaaacg cagatgttta gataggctgc gtcattaaac
     4921 caaccgattt cacacccgca ctatgtaaca agttcagtgc tttaattatt tcatcgtaag
     4981 gcacatcttt tgcgccaccg atcagaaaga ccgttttcgg gttggccttg aaacggctgg
     5041 acacttccgc caccacctgc tctggtggta aacgctccag gcgatctttc tcaaccacca
     5101 cggtgtactg accaatacca gacacttcaa caatcactgg cggattatcg ttactgctca
     5161 ccgcctgtga ttcagtagcg tctggcagat cgacctccac gctctgggtg atgatgggcg
     5221 ctgtcgccat aaagatcagc aacagcacca gcagtacgtc cagcaacggt acaatgttga
     5281 tttcggactt gagatcgcga cgacctcgtc cacgcgctct ggccatggct taccccttgt
     5341 tgctctcgct aacggtaaac gcctggcggt gcagaatcgc ggtaaactct tccataaagt
     5401 tgtcgtaatt cagttccagt ttgtttacgc gctggttgag gcggttataa gccattaccg
     5461 ccggaattgc ggcgaacaga ccaattgcag tcgcaatcaa cgcttctgcg ataccgggcg
     5521 caaccatttg cagtgttgct tgttttaccg ccccgagggc gataaaggcg tgcatgatcc
     5581 cccagaccgt accaaacaga ccaatatacg ggctgatgga gccaaccgta ccgaggaacg
     5641 gaatgtgcgt ttccagattt tcaagttcac ggttcatgga gatacgcata gcacgcgacg
     5701 ccccttccac tacggcttcc ggcgcatggc tattggcacg atgcaggcgc acaaactctt
     5761 tgaacccgct gtaaaatatt tgttccgaac cagtcagatt atcccgtttc ccctgactct
     5821 cttgatagag gcgagagagt tcgattccag accagaattt atcttcaaac gcttcggctt
     5881 cgcgcgccgc tgcgttaaga atacgggtcc gctggataat aatggcccag gatgcgattg
     5941 aaaaaccaat caaaatcaac atgataagtt taaccagaag gctagccttc aggaacaaat
     6001 caaggatatt catgtcagtc actgcttaaa ctccgcgaca atagacttgg gaagcgcacg
     6061 aggcttcatt ttgagtgggt caacgcaaac aaccagaacc tctgcttcat tcagcaaagt
     6121 attctcggcg ttgacaatac gttgcgtgaa aaccaaagag gtgccacgca ttgatgttat
     6181 ttcagtctgt atttcgagca tatcgtcgag ccgcgcaggt gcgtaatatt ccaccgtcat
     6241 tttacgtacc acaaaggcaa cgcgttcagc catcagcgcc tgctgactga agtgatgatg
     6301 acgcagcatc tctgtgcgtg ctctttcata aaaagcgacg taactggcgt ggtacaccac
     6361 accaccggca tcggtatctt catagtagac gcgaaccggc catcgaaaca gcgttgtatt
     6421 cactttacat cccggtaatg caacaaaagt tagagctttt aaacgctgct actatacgcg
     6481 cgcgattgag gtttgggaat gagggcaagt taagggagcg taaaaattta tgggcctgga
     6541 gcggcccata attttttaaa gagaattctg atttaaaaga agaagaaaat cagcccgaca
     6601 atgagaacaa tatcggcaag caatgggcaa aaaatccctt gccaaagaac cttctgcgga
     6661 cgaaagccca cgccgtgaat cacaccagca cagacggccc acatcagcaa taaaccatgc
     6721 cagatttcca gttcactggt cttcgcggca aaacgagatg ggtcccaaaa catacatcct
     6781 gctaacagaa gcgccatcac gaaggaaagc gcccgcaggg ggcgcttgtc cattaccgca
     6841 tacaaggtcg cgataatctt actcatcaga tgtcttcttg accggctttg cctgattcga
     6901 cgtgttcaag cgccagcgcg gtgattaccc caaacgaaca ggcaagaagc gttcccagaa
     6961 tccatgcgaa ataccacatt tttagctcct tacttagtac agagagtggg tgttacgttc
     7021 aatatcttct ttggtgatac gaccgaacat tttccagtaa caccaagcgg tgtagagcag
     7081 aatgatcggt accagaacca ccgcaaccca ggtcatgacg ttaagcgtca gctggctgga
     7141 agttgcatcc cacattgtca gacttgcgtt catcatggtg ctggacggca tcacaaacgg
     7201 gaacattgcg ataccggctg tcaggatgat gcaggccagc gtcagggagg agaacacaaa
     7261 cgcccacgcg gctttatcca tacgtgcagt caggatggtc agcagcggaa gaaccacacc
     7321 cagtgccgga atagcccaca gaattggcgt gttgttgaag ttaaccagcc atgcgccagc
     7381 ttcacgaacc acttctttat tcagtgggtt agaggctgcg taatggtcca ttgtcgattt
     7441 cacgacataa ccatcgatac cgtacatcac ccatacgcca gccagtgcga aacagaccag
     7501 tgtcaccagc gcagccacct gagccgttgc acgggtacgc aggtgcagtt cgcccacggt
     7561 acgcatttgc agataggttg cgccctgagt aatgatcatc cctacgctca ccacgcctgc
     7621 cagcaggccg aacgggttaa gcaactggaa gaagttaccg gtgtagtaca gacgcagata
     7681 ttcatcaacg ttgaacggta cgccctgcaa caggttaccg aacgctacac caattaccag
     7741 cggcggaacg aaactaccaa tgaagatgcc ccagtcccac atgttacgcc agcgggtttc
     7801 ttcaatcttg gagcggtagt caaaaccgac cggacggaag aacaaagacg ccagcacgag
     7861 gatcatcgcc acatagaagc cggagaacgc agcggcatag accatcggcc aggcagcaaa
     7921 gagtgcgccg cccgcggtga tcagccaaac ctggttaccg tcccagtgtg gtgcaatgga
     7981 gttaatcata attcgacgct cggtgtcgtt acgaccgagg aaacgggtga gcatgcccac
     8041 ccccatgtcg aaaccgtcag tgactgcaaa accaatcagc agaacgccaa ccagcagcca
     8101 ccagataaaa cgcaatactt cataatcgat catttgacga ctcctgtctt agcgtgccgg
     8161 ctgagtagtc gtggaagact gctcaaagtg atagcgaccg gttttcaggc tgcttgggcc
     8221 gaggcgtgca aacttgaaca ttaagaacaa ttctgccacc aggaacaggg tatacaggcc
     8281 gcaaatcagc accattgaga agatgagatc gcctgcggtc agtgacgagt tcgccacagc
     8341 tgtcggcagc acttcaccga tagcccacgg ttggcggcca tattcagcca cgaaccagcc
     8401 cgcttctaca gcaatccacg gcagcggaat accgtacagc gcggcgcgca gaagccattt
     8461 tttctcgcca atgcggttgc ggatgacact ccagaaagag agcgcgatga ttgccagaag
     8521 caggaagcca cacgccacca tgatacggaa cgcaaagtac agcggcgcta cacgcgggat
     8581 ggagtctttg gttgcctgtt gaatctgcgc ttcagtcgca tcagccacgt ttggcgtata
     8641 gcgtttcagc agcagaccgt aaccgaggtc tttcttcatg ctattgaact ggtcacgaac
     8701 cgcctggtcg gtagaaccag aacgcagttg ttcgagcaga gagtacgcct tcatcccgtt
     8761 acgaatgcgt tcttcatgct gcaccatcag ctctttcagg ccgataaccg gggtatccac
     8821 ggaacgcgtt gcaatgatgc ccagtgcgta agggatctga atcgcaaatt tgttcgtctc
     8881 ctcttcctga tcaggaatgc cgaacagagt aaaggcagca ggcgcaggtt gcgtttccca
     8941 ctcggcttca atagcagcca gtttggtttt ctgcacgtcg cccatttcgt agccggattc
     9001 atcacccaga acaataacag acagaacagc agccataccg aagctggcag cgatagcaaa
     9061 ggagcgttta gcgaaggcga agtcacgacc tttcagcata taccatgcgc tgataccgag
     9121 gatgaacatc gcgccagtca cataaccaga cgctacagtg tgaacgaatt tcacctgagc
     9181 aaccgggtta agcaccagct cggagaagct caccatctcc atacgcatag tttcaaagtt
     9241 gaaatcggac gcgattgggt tttgcatcca gccgttcgca accagaatcc acagtgcgga
     9301 caggtttgaa ccgagcgcca ccagccaggt gacacacata tgctgaactt tacccagacg
     9361 atcccaaccg aagaagaaca gacctacaaa ggtggattcg aggaagaagg ccatcagacc
     9421 ttcgattgcc agcggcgcac cgaagatatc ccctacatag tgggaatagt aagaccagtt
     9481 agtcccgaac tggaactcca tggtcagacc ggtagccaca cccagagcga agttgatacc
     9541 aaacaacttg ccccagaact tggtcatatc tttataaatc tgtttgccgg agaggacgta
     9601 gaccgtttcc ataatggcca gcaggaacgc cataccgagc gtcagtggca caaaaaggaa
     9661 gtggtacatc gcggtcaagg caaactgtaa gcgcgacagt tcgactatat ctaacatcat
     9721 gactccttgc tcatcgcatg aagactccga gagtgaaccc cgttagaaag ggtcacacgc
     9781 atgccccaat agaaatttgt ttgaacactt tcatcatacc atgtgaatgc acacaaaaac
     9841 ggcgtaaaga aaggttgcaa acatgttaat aaaaactcaa attgatccca cgtatatatt
     9901 acgccgcaaa atccttacaa taaacaggtt tttgttgaag caaatttgca tttttcgacg
     9961 gtgatcaatt tatagctaaa ttaccgcctt tcagccaatt tgatcgagaa caatttatct
    10021 ctttttgatg ccaatttcca agacttatac attgataaat atcaattcct acaaattatg
    10081 ttaatcatgt ttcaataagg taactttagt gacatttatg tttaaaatgt gtgagttata
    10141 aggctgaaat tagtttcggt tggcaattta aagataaaaa gagacgaagc aaaaacagca
    10201 cggttgacct taattctttt tttggtcact ggctaatacg acgcagagtt tcaggattac
    10261 taataaaggc ataaatccac cccgccgata ttaacattta attaacttgt aattatatta
    10321 taattaactc acccagggtt attattttgt taaaaataaa gccacatata cgtggcttga
    10381 gagtgaagat aaacgcaaaa ttacctgatg cgctacgctt gccagtgtct acatcatctc
    10441 tgcaatatat tgaatttgcc tgcttttgta gaccgaataa agcgttcatg ccgtatccgg
    10501 catgaacaaa gcgccctttg tcaacaatct ggccgcgcat atgtggcttc ggtttgattg
    10561 ctattcaggc aagccggtaa ctgaacgtcc gtgactggcc gggtaaaaaa ggccccgata
    10621 gatttgaacc ttgattttcc tcggtggtaa tgtgttcatc catcatcgtt tctgagcaag
    10681 aaatcacctc gcgactgaaa gcaacagtcg catcacaggt tgctgattca gccggattaa
    10741 acagccgtaa aatcacttct tgtcggtctt cagctttctt aagtgcgctt atcaggcatc
    10801 ccactggggg cattttcaac aaactataac tttccggcac gttgaatccg gctttgttga
    10861 gcttcatcgc atcccatggg attttgttgt aacactgtac tggagtcagc catgctcgcg
    10921 cctgctgagc tacaccagcg gcggttggcg taccggtata actcaataaa cttaggcgac
    10981 aagaaagcag accacgtagt tgtgagtccg ggactggcat tttaattccc gaaggccgcc
    11041 caggccttaa aagcagatct tctttgccca gtaagcccac gccacgcagc aacgtaatgg
    11101 caaaggtttt cttctcttca ccgatgactt caaattcacg taacccttcg ctaaagacag
    11161 ccatgccgtt acgcccttcc tgtaaggcaa catagttgag catattccat accggaaccg
    11221 gcgcttcttt ccagccttct tgctgccagt tgttcattgc actgtcgttc acggggcgcg
    11281 ttagcgaacc aaactgcgta tctgccagaa cactgtcagt gttaaaaggt gtagggacca
    11341 ggacacgaag gcgatgatcg tcagcctggt tatcaaggtt gatatccaca tcaatacgcc
    11401 tgctgttatg actaagagtg acaaccaaca ccccccctac tctgccagtg gattgccggg
    11461 cgctgcgttc tgacaaattg agcggcactg ccatgtcata gcggataaca gccctgctct
    11521 gccaggcttc atgaataata tcgcattgcg gtttcgcgtt cgctgcggta attacccact
    11581 cttcttttgc gggtgaatag tcatattcat caccatcatc agagctttct tcaatttgca
    11641 atacccggtc atagcgcaca ccgctgtctt tatctaccag ttgcagagaa ccatcctcat
    11701 tgagcgcaat ctgccagaaa gcattttcca gaatcccttc agcgtcactt tttgccgcaa
    11761 tcacgttgcc aggctgattc gcttcaatat aaagcgtgcg atagcccata gaagggacaa
    11821 tctggttgat ctgaatatca aactccataa agggatcata attgccataa tgaacaattt
    11881 gccgatcgat taggccagga tcgatctcac gggcatggcg aataaaatac ggtacaggct
    11941 gaccgcgatc gtcttgtaga ttaaactggc tggcgcgcag ccgcacagtg gtgttaataa
    12001 cttcttcccg cggccagggc atcaggttaa acagcacgag tttgtcggca tcactctgtg
    12061 gcatgttatc ggcaattttg cgcatgtaga aacgcagcag attatccgcc atgtcttcag
    12121 ccagttcgaa gcgggcgaca atttcgcgat gaactttgtc actgcaacaa caaccgatac
    12181 tgtcgtgggc atgatttttt aagatctctt tccacatttt ctccagcaag ccgtggtggt
    12241 attcaaaacc caacgtccag gccagcgttg ccagcggttc cagcagatta acaatcttat
    12301 tttcaatacg tgcgtgggca attttgatat ccatgcgcgt ggaaccgatg gtgcgatgta
    12361 cgcgcatata tttgccatcg ataaattcac ctttcagggt ggccagactt tcacgctgcg
    12421 cttcgatctt ctcaaatacc tcttcaaagc ggctcatcac aaacttacgt tgagggtaga
    12481 tctcacgtag cttatccatc acttcgaaga tattttgctg caatggcatc tggtcatgac
    12541 cattcggcag caaaatctct ttggttacag acgctttttc cagcacgtca aaataactgt
    12601 cgaggcgttt acgtaatccg ttttcgtcgg caggtaagta cttaccgatg gcgtagccca
    12661 gcggcagcac ctgcgccgtc acttcgctac cgtcactgct ttgccacaaa aactcggttt
    12721 tatcagtacc gtggcgctcc gaacatccgc gccagaacat agtgcgggta atgccaaatc
    12781 cattgtaaat atgcggcagt tgcccggaca tgccaaagga atccggtaaa taacctattt
    12841 tcatcggctc gccaaacgcg aggcagtcac gcattccgta catcagatta cggacgatgg
    12901 attccgcaga aacaatcgtg gtatcggtct gggtatacca ggggccgata atcaacttac
    12961 cggcttctac ctgtttcttc acgcggtctt tgttttccgg tttcaccgcg aaataatctt
    13021 cgaggatcgc cgtttgcccg tcgagtacgt aatatttgta ttcgttgtcc tgttccagtc
    13081 ggcacaggat ctcttccata ttattgacca gcagaatgcg tgactcttcg gtggtgaaat
    13141 accactcccg atcccaatgc atatgcgggg tgatgtgaac gcgagatacc gctttcatcg
    13201 tcgtttcctt tttgtttatg gcattacgcc atcagtcaga tagttgccat gcttaaccgc
    13261 gtgacgccgc cagatcagga gaattgcagt cgagattgca gcccccacca atgccgcacc
    13321 aaaccagcca attgccgcca taacaccgcc cgcgccatta tcatgaagta aaaagagcga
    13381 gaaaatgccg gcaccgggtg tcgaaaggcc gatattcatc gcaccgacaa tagcgcccgt
    13441 taccatagag cccagcacaa acgaaccaat aacccgcagc ggatcttcaa tcgccatcgg
    13501 gatcgcccct tcggtaatac ctgccagccc taacagccag gtggatttcc cggtctcaat
    13561 ttcaaactct ttaaacaggc gcggtgcgag catcgtggaa gcggttacgg taaatgccga
    13621 aaccattttg acggaggcga aaatggcata cgggccgtaa acgccgttcg ccattgcgcc
    13681 caggcagaat gcataagcgg ctttgttcac tggccctcca aggtcaaagg aacacataaa
    13741 accgagaatg gcacccagca acagcgcgtt acttcctgac agaccgttca gccaggcggt
    13801 aagcgagtta ttgatccatg cgacaggttc tcccaccaca aacagcatca gactgcccgc
    13861 tcccaacgta ccgagcaccg ggtagagata aaaagtcagg aatccattga atttactgct
    13921 aagacgcaag tgatttttca cccagcgcat caagtaaccg gctatcaatc cgccaacgac
    13981 cgcgccgaga aacccggagc cgatcatgtt ggcggcaagt ccagccgcaa agcctggcgc
    14041 taacgccggt ttatctgcca gagaataggc ggtataggcc gcgagcaccg gtaccatcaa
    14101 aattccgagc agcccgccgc ccagcttgcg gtacatccac agccaggagt tttcttcatt
    14161 aaacagatct tgtagcccga agatttgcga cagtaatacc gcgaccgcca gcaccgtgcc
    14221 ccccgcgaca atcaacggta cggcaaaaga gattccgctc aacagtgcct gtttcagctc
    14281 cgttttgaca cttttcaccg gttgcgtatc ttgctgtacg gtacgcgtct catcgctacg
    14341 cttgagggta agcgcttgtt ggatcaacgc ttctgcatgg cgaatcggct cggcaacagg
    14401 cactgaaagc gcgggaatgc cattaaaacg ctcactctcc ttgatggcga cttcagccgc
    14461 aaaaatacag gcggttgcac tattgagttg atccgccgtt aaacgccctt caatgccgtt
    14521 agcgccttgt ttttcaacgt aaacatttac gccgagtttg cgtccggctt tttccagata
    14581 ttccgcagcc atataggtgt gagcaatacc cgccggacag gccgttacgc agacgatagt
    14641 tggcgcgttg gaaaaagagg cagaaggttg cgtgcctccc ttgtcatcca gcgccgagag
    14701 caactcatca ggcgtcgtcg ccgactgtat acgcgcccga atctcatcat ccgcaaggcg
    14761 cgtggtcagc gctgtcagca gttgcatatg cgtagtaccc gcttcattgg ggggaatcgc
    14821 cagcagcacc actaaatcaa ctgcttccgg gccatcaacg ccttcccact gaagcggctc
    14881 gctgagtgtg gcgaccgcaa acgccgcttc ttttaccgca gcagttttgc catgcggcac
    14941 agccaaccct tcacctaagg cggtcgggcc aaggctttca cggcgataca cttcttccag
    15001 aaattgctca gtactggaaa ttttccccag agcagcaaga cgttgagtca acgcgtggat
    15061 ggcctcttca cggctggtaa agcgcgcatt cagacacaac gcatcgcggt gggtcagagt
    15121 cgtcagattc atcatgatcc tcacttgatc gtttttatag tcgcgccagt gtgcccgata
    15181 aaacaatacc atcatgtgat caatttcact tgattgaaat tatttgtatt gtatttgtat
    15241 tatttacagg atgaatttat cgggcataat ggctaaattc gtattaatga gatactaaaa
    15301 atgggacaca agcccttata ccggcagatt gccgatcgca ttcgtgaaca aattgcgcgt
    15361 ggcgagctta aacctggcga tgcgttaccc actgaatcag cgctacaaac agagtttggc
    15421 gtcagccggg ttacggtgcg tcaggctttg cgccagctag tcgagcagca gatcctcgaa
    15481 agcattcagg gcagcgggac ttacgtcaaa gaagagcggg tcaattacga tatttttcag
    15541 ttaaccagtt ttgatgaaaa actgtcggac cgtcacgtcg atacacacag tgaaatcctg
    15601 atatttgaag tgattccggc tgacgatttt cttcagcaac agctacaaat caccgcgcag
    15661 gatcgtgtgt ggcatgtgaa gcgcgtgcgc tatcgcaagc aaaagccaat ggcgctggaa
    15721 gaaacctgga tgccgcttgc gttgttcccg gatctcacct ggcaggtcat ggaaaattcg
    15781 aaatatcact ttattgaaga agtgaagaag atggttatcg atcgtagcga acaggaaatc
    15841 attcctctga tgccaaccga agagatgagc cgtctgctta atatcagcca gacaaagccg
    15901 attcttgaaa aagtatcgcg tggatattta gttgatggtc gggtgtttga atatagccgc
    15961 aacgctttta ataccgatga ctataaattt accctgatag cgcagcgaaa atcatcgcga
    16021 taagcacaaa aaaggccatc atacgatggc caaccatgtc gaaaccgaca gcccttcgcc
    16081 attcctgtag agggaagcgg cgagggctat ttcttattac agatatttat ttcagaacag
    16141 ttttcagtgc ttcaccgata tccgccaggc tgcgaacggt tttcacgcct gcggcttcca
    16201 gagcagcgaa tttctcatcc gcagtccctt tcccaccagc aatgatcgca cccgcgtggc
    16261 ccatacgttt gcctttcggc gcagtcacac cagcgatgta acctacaact ggcttggtaa
    16321 cgtgctcttt gatgtacgct gccgcttctt cttcagcgct accgccgatc tcaccgatca
    16381 tcacgatcgc ttcggtctgc ggatcttttt cgaacatttc gaggatgtcg ataaagttag
    16441 agcctgggat cgggtcaccg ccgataccaa cacaggtcga ctgaccgaaa ccgtaatccg
    16501 tggtctgttt aaccgcttca taggtcagtg taccggaacg ggaaacgata cccactttac
    16561 ccggtttgtg aatgtgaccc ggctggatac cgattttgca ttcacccgga gtgataacgc
    16621 ccgggcagtt cgggccgatc atacgaacgc cggcttcgtc cagtttcact ttcacagtca
    16681 gcatatccag cgtcgggatg ccttcagtga tggtgataat cagtttgatg cctgcatcaa
    16741 tagcttccag aatggagtct ttgcagaacg gtgctggtac gtagataaca gaagcggtag
    16801 cgccagtagc agcaacagct tcacgcacgg tgttgaacac cggcaggccg aggtgggtag
    16861 taccgccttt acctggggtt acgccgccaa ccattttagt gccgtatgca atggcctgtt
    16921 ctgagtggaa agtcccctgg ctaccagtaa agccctggca gataaccttg gtgtttttat
    16981 caattaaaat ggacattatt tcccctccac tgcggcaaca acctgctgag ctgcatccgt
    17041 cagacctttt gctgcaataa tattcaggcc gctgtcagcc agtttcttcg cgccgagttc
    17101 ggcgttgtta ccttccagac gtaccacgac cggtacgtta acacccactt ctgctaccgc
    17161 gccgatgata ccgtcagcga tcaggtcgca acgaacgata ccgccgaaga tgttaaccag
    17221 aacggctttc actttgtcgt cagagaggat gattttgaac gcttcggtta cacgttcttt
    17281 ggttgcgccg ccgccaacgt caaggaagtt agccggttcg ccgccgtgca gtttaacgat
    17341 gtccatcgta cccatcgcca gacctgcgcc gttaaccata caaccgatgt taccgtccag
    17401 cgcaacgtag ttcagttccc actgtgcagc ctgtgcttca cgcggatctt cctgcgactg
    17461 gtcacgcatt tcgcgcagat caggctggcg gaacagtgcg ttgccgtcag cgcccagttt
    17521 gccgtcgagg caaatcagat cgccctgttt ggtgatgacc agcgggttga tttcgatcaa
    17581 cgccaggtcg cgctccagga aaatggtcgc caggcccatg aagattttgg tgaactgctg
    17641 aaccagttta ccttccagac ccagtttgaa cgccagctcg cgtccctgat acggcatcgg
    17701 gccagtcagc ggatcaagcg caactttgtg gatcaggtgc ggagtttctt ccgccacttt
    17761 ttcgatttcc acgccgcctt cggtggaggc cataaagacc acacgacggg aactacggtc
    17821 tacaacggca ccgagataca gctctttagc gatatcggtc gctgcttcaa ccagaatctg
    17881 gttaaccggt tggccattgg catctgtttg atacgttacc agacgcttgc cgagccagtt
    17941 ttctgcaaaa gcacggatgt cttctttgct gtttacaact ttcacaccgc ccgctttacc
    18001 gcggccacca gcgtgaacct gacatttcac tacccacggg ccggcaccga tttttgaagc
    18061 ggcttcttct gcttcgcgcg gagtagtaca ggcataaccc accggtgctg gtaagccata
    18121 gcgggcaaaa agttgttttg cctgatattc atgtaagttc atgtgttctg tccatccttc
    18181 agtaatcgtt atcttttaaa ccgtagaccg acaccctatc cacgtcggtc tacagtgcag
    18241 gtgaagctta aactactaca cgtccagcag cagacgcgtc ggatcttcca gcaactcttt
    18301 gatcgttacc aggaagccca cggattcgcg accatcgatc agacggtgat cgtaggacag
    18361 cgccaggtac atcatcggca ggatctcaac ctgaccattc accgccatcg gacgatcttt
    18421 gatagcgtgc atacccagaa ttgcgctctg cggcgggttg atgatcggcg tagacatcag
    18481 ggaaccgaac acaccaccgt tggtgatggt gaagttacca ccggtcagat cttcaaccgt
    18541 cagcttgccg tcacggcctt taactgccag ctctttgatt ttcttctcga tgtctgccat
    18601 gccgagggta tcgacatcac gcagtaccgg cgtcaccagg ccgcgcggcg tggaaaccgc
    18661 catgctgacg tcgaaatagt tgtggtaaac cacgtcatca ccgtcgatag aagcattcac
    18721 ttccgggtaa cgtttcaggg cttcaaccac cgctttcacg tagaaggaca taaagcccag
    18781 acggatgccg tggcgttttt caaacgcttc accgtactgc ttacgcagat ccataatcgg
    18841 cttcatgttg acttcgttga acgtggtcag catggcggtg gagtttttcg cttccagcag
    18901 acgctctgcc acacgcttac gcaggcgagt catcgggaca cgtttttcac tacgtgcagc
    18961 cagagccggt tgcgccgccg gagcagccgc tgccggagca gactctttcg ccggggcttt
    19021 cgccagatgt ttttccacat cttcacgagt cagacgacca cccacaccgg tgcctttaat
    19081 ggcgctggcg tcgagattgt gttcagccag caggcgacgg atcgccgggc ttaacgcatc
    19141 gttgttttgc tcttccagag acgcctgctg gcgttgcgcc ggagtggacg ctttctcttc
    19201 agatttggcg ctggtttctt taccggcgct gttgccttca cgcaggcgac caaggatctg
    19261 acgagacgtt accgttgtac cttcatcttc cagaaccgca tccagaatgc cgtctgctga
    19321 tgccggtact tccagtacca ctttgtcagt ttcgatttct accagcactt catcacgtac
    19381 gactgcgtcg ccgggttttt tatgccaggt tgcgacggtg gcatcggcta cggattcagg
    19441 caggtcaggg accagaatat ctacgctact cattgtgtat cctttattta ttcgacgttc
    19501 agcgcgtcat taaccagatc ttgttgctgt ttctggtgaa cggacatata ccctaccgcc
    19561 ggagaggcgg aggccgtgcg gcctgcataa cgcagagaag ccccaaacgg aatcacttca
    19621 cggaaatgat gctggctgca gtaccatgcg ccctggttga gcggctcttc ctggcaccag
    19681 acaaaatcct tgacgtgagc aaactgctgc aacacttcct gcatcgcttt atgcgggaac
    19741 gggtagagtt gctcgatacg cacaatggcg acatcgtgtt gattgttctt acgacgctgt
    19801 tccagcaggt cgtaataaac cttaccagaa cacatcacta cgcgcttcac gcccttcgga
    19861 tcaagctcgt cgatttcacc gatggctggc aggaaggtgc cgttcgccag ttcttcgagg
    19921 ctggaaaccg ccagcggatg acgcagcagg gatttcggcg acatcactac cagcggacga
    19981 cgcatcccgc gcagcgcctg acgacgcagc atgtggtaaa cctgtgccgg ggtagacgga
    20041 acgcaaacct gcatgttttg ctcagcacaa agttgcagat aacgttccag acgcgcggag
    20101 gagtgctccg gcccctgccc ttcgtaaccg tgcggcagca acatcaccag accacacatc
    20161 cggccccatt tctgttcgcc agaagagatg aactggtcga taaccacctg cgcaccgttg
    20221 gcgaagtcac cgaactgcgc ttcccagatg gtcagagtgc gtggttctgc ggtggcataa
    20281 ccgtattcaa acgccagcac tgcttcttca gacagtacgg agtcccagac gcggaacgcg
    20341 ccctgcccgt tatggatgtg ttgcaacggc gtgtaagtgg aaccgttaga ctggttgtgg
    20401 atcaccgcgt ggcggtggaa gaaggtaccg cgaccggagt cttcacccga caggcgaacc
    20461 ggaatgcctt cgtcaaccag cgtggcgtaa gcgaggtttt ccgcaccgcc ccagtcgaac
    20521 agtttctcac cggcagccat cgcctggcga tcgccataaa tcttggcaac gcgagactgc
    20581 atttcaactg cttccggcac cgtgctgatg cgtttcgcca gctcctgcag gcgcttcatc
    20641 tcaactttgt tcgggtactc ttcgtcccat tcgtggttga ggtacggcga ccaggtgaaa
    20701 gagtgcatgt tcatcggacg ccactctgcc actacgcaat cgccagcatc cagcgcatcg
    20761 cggtacaggt taaccatctc ggtggcatct tccagcgtcg ccactttttc ctgctccagc
    20821 ttgtcagcgt agattttgcg cggtgtcgga tgttttttga ttttctgata catcagcggc
    20881 tgggttgcgc tcggctcgtc ggcttcgttg tggccgtgac ggcggtagca caccaggtcg
    20941 atgaagacat cacgtttaaa ggtgttacgg aaatcgagcg ccagacgggt cacaaaggca
    21001 acggcttccg gatcatccgc gttaacgtgg aaaatcggtg cctgaaccat cttaccgata
    21061 tcagtacagt acggtgtaga acgagcatcc agtggattag aggtggtgaa accaacctgg
    21121 ttgttgataa cgatacgtac cgtaccgcca acttcataac cacgcgcttt cgacatgttc
    21181 agagtttcct gaaccacgcc ctgcccggtc actgcggcgt caccgtggat ggtgattggc
    21241 agcactttgt tgctgctcgg ctcatcaagt ctgtccagac gggcacgaac agaaccgata
    21301 actaccgggc ttacaatctc aaggtgagac gggttaaacg ccagcgccag gtgcaccagg
    21361 ccgccatcgg tctggaagtc agacgagaag cccatgtggt atttcacgtc acccgtgccg
    21421 aggtgttctt tatgtttacc ggcaaactcg tcgaacaagt cttgcggttt tttacccagc
    21481 acgttcacca gcacgttcag acgaccacgg tgcgccatcc caagaaccac ttcgcgggtg
    21541 ccgctgttgc cagcgtggcg gatcatctct ttaagcatcg ggattaacgc gtcaccgcct
    21601 tccagcgaga agcgttttgc gccagggaat tttgcgccga ggtaacgttc aaggccttca
    21661 gcggcggtca gttcgcttaa gaagcgtttt ttctcttcgc tattgaaagt cgcgcgacca
    21721 gactcgatac gctgttggat ccagcgtttt tcttcggtgc tggtaatgtg catatactcg
    21781 gcaccaatcg ggccacagta ggtttgcttg agggcttcca gcagctcgcc gagtttcatg
    21841 ttttctttgc cgctggcaaa tgaaccgacg ttgaaggtct cctggaagtc tgcttcggtc
    21901 agatcgtgga aagacggatc cagatcggcc actttatctt gctgccacag tcccagcgga
    21961 tcgagattcg catgctggtg accacggaag cggtatgcgt taatgagctg caggacttta
    22021 acctgcttca cattggtgtc agggtcggag atcgttgaag agtaacgtga agcgtctttc
    22081 gccaggcggc ggaaatattc acgcgtttga gagtggaatt gatccggttt gactcccgta
    22141 ccaggtaact gctggaacgt cgaacgccag ttagcgtcaa ccgagtcagg atcggttaag
    22201 aagtcttcat agagctgttc tatccagctc tggtttgcgc cagagaggta agaagagtcc
    22261 aaccaggctt tcaaagcgct gttctgcatc gtgatccctt aagcatcttt tttatgctta
    22321 cttcgccgtg gatactacca cgcacagtga tgtgcgtgcc ggggttcact tgcgagctct
    22381 tgtctatgta gtggctcgcg aaggaacctt taaaaactgt cttagtgtca ggggcagttt
    22441 ttaaagattt cctgcatttc tttgttacgc ctgatgcgct gcgcttatca ggcctacggg
    22501 taatgacttg taggccggat aaggcgttca cgccgcatcc ggcactggtt gcctgatgcg
    22561 acgcttgcgc gtcttatcag gcctacggtt tacgcattac gttgcaacaa catcgactta
    22621 atatggccga tggcgcgcgt cgggttcagc cccttcggac atacactgac gcagttcatg
    22681 atgctgtgac agcggaatac gctgaatgca tcgctcaaac cgtcgaggcg gctgtcagtc
    22741 tcggtatcac ggctatcgat caggaaacga tatgccgcta acaagcctgc cgggccgata
    22801 aacttatcgg gattccacca gaaagacgga caagaggttg aacaacatgc gcagagaata
    22861 cattcataca gcccgtcgag tttttcgcgc tgctctggca tctgtaaatg ctcgcgagct
    22921 ggcggatttt gtccattatt caacaggtaa ggcttaattt tctcatattg cgcatagaat
    22981 tgtcccatgt ctaccaccaa atcgcggatc accggtaaac ctggcagcgg gcgaatcaca
    23041 atcttcttgc ccggctggtt gagtgccgaa atcggggtaa tacaggccag accattcttg
    23101 ccgttcatgt tcagaccgtc ggaaccgcac acaccttcac ggcaggagcg gcggaacgac
    23161 aggctgggat ctttctcttt cagctggata agcgcatcca gcagcatcat gtcgcgacct
    23221 tcttccgctt ccagggtgta atcctgcata cgcggagcat catcaacatc cgggttatag
    23281 cgataaattg aaaactcgag tctcattttc ctgtctccgc attagtaagt acgaatcttc
    23341 ggcgggaatg ccgggcgcag tttcggttcc atgttgacgc ttcggcgcgt catggattcc
    23401 gactctggca gatacaggga gtggcacagc cagttttcat catcacgatc cgggaagtcg
    23461 aagcggctat gtgcgccacg gctttcggta cggaagttgg cagaaacagc cgttgcatac
    23521 gccgtttcca tcaggttatc cagttccagg cactcaacgc gctgggtgtt gaactcgctg
    23581 gaagtgtcat ccagacgggc atttttcaga cgctcgcgga tcactttcaa ctgctcaagc
    23641 cctttcgcca tcgcatcacc ttcacggaag accgagaagt tatgctgcat acattcttgc
    23701 agcgctttac gaatcgccac cggatcttca ccgttacgat tattgttcca gcggttcaga
    23761 cgatccagag atgcttcaac atcagactcg ctggcatcgc gcaatgcgcc ctgttcggcg
    23821 atagactctt gcagatgcag acctgccgca cgaccaaaga ccaccaggtc cagcagcgag
    23881 ttgccgccca gacggttagc gccgtgtacc gatacacaag cgatttcacc aacggcaaac
    23941 agccccggaa caaccacatc ttcgcctttc tcgttcaccg tcagcgcctg accggtcact
    24001 ttggtcggaa taccgcccat catgtagtga caggttggga taaccggaat cggctctttc
    24061 accggatcga cgtgagcaaa ggtacgggaa agctccagga tacccggcag acgggattcg
    24121 agaacttctt tacccaggtg atcgagtttc agtttcgcgt gtggccccca cggaccatca
    24181 cagccgcgac cttcacggat ttcaatcatg atggaacgcg ccaccacatc acggcccgcc
    24241 aggtctttgg cgttcggcgc ataacgctcc ataaagcgtt cgccatgttt gttcagcaga
    24301 taaccgcctt caccacggca accttcggtg accagtacgc ccgcaccggc aatgccggtc
    24361 gggtggaatt gccacatttc catatcctgc accggtacgc ctgcgcggat agccatgccg
    24421 acaccgtcgc cagtgttaat gtgggcgtta gtggtggact gataaatacg ccctgccccg
    24481 ccagtcgcca gcacggtagc gcgggctttg aaataaacca cttcaccggt ttcgatgcac
    24541 agtgcggtac aacccaccac cgcgccatcc tggtttttca ccagatccag cgcataccac
    24601 tcggagaaaa tggtggtgtg gtttttcagg ttctgctgat aaagcgtgtg caacagtgcg
    24661 tgaccggtac ggtcagccgc cgccgcagtg cgtgccgcct gctcgccgcc gaagtttttc
    24721 gactgaccgc caaacggacg ttgatagata cgaccatcat caagacgcga gaacggcagg
    24781 cccatgtgtt cgagttccag aatcgcttcc ggcccggttt tacacatata ttcaatcgcg
    24841 tcctggtcac cgatatagtc cgaccctttt acggtgtcgt acatatgcca ttcccagtta
    24901 tcttcatggg tattacccag cgcaacggta atgccgcctt gcgcagaaac ggtatgggaa
    24961 cgggtcggga agactttaga gagcagcgca caggtctggc cgctctggga aatttgcagc
    25021 gccgcgcgca tacctgcgcc accggcacca atcacaactg catcaaattc tctgactggc
    25081 aatttcatca cacaccccac accacaacga atccataaat cacgtaaacc accagtgcaa
    25141 cgacaatcac cagttgcagc atcaggcgca atgccagcgg tttaacgtag tcggtcaaca
    25201 cctgccacat gccgatccag gcatgaatca agatagaaaa cagcgccagc agggtgaaca
    25261 ctttggtgaa cgcagaggcg aagaaaccga tccagacttc atatgtcagt tcgccactgg
    25321 tggcgaaaaa accgaccata taaatgatgt agagcgtcag gacgatagcg gtagcgcgaa
    25381 cgaggatgaa atcatgtacg ccattgcgtc ctaatgcgga ggcgttgctt accatacgag
    25441 gactcctgcg agaagtgaaa gcacgacagt aataacaaag gagattttgg cggagcgttt
    25501 acccgcttcg aatgtttctt ccagatagcc aaaatccatc atcatgtggc gaatacctac
    25561 gacgacgtga tacgccagag cggtaaggat gccccacatg ataaatttga cgaagaagct
    25621 gcccataatc gcggaagctt gctcgaaacc ttcaggggaa gagaggctgg tacccagaag
    25681 ccacagcagg atgcccactg caacaaaggt gatcacaccg gaaacgcgat ggagaatgga
    25741 cgctatcgcc gtgatgggga accggatggt ctgtaggtcc agattaacag gtctttgttt
    25801 tttcacattt cttatcatga ataacgccca catgctgttc ttattattcc ctggggacta
    25861 cgggcacaga ggttaacttt ctgttacctg gagacgtcgg gatttccttc ctccggtctg
    25921 cttgcgggtc agacagcgtc ctttctataa ctgcgcgtca tgcaaaacac tgcttccaga
    25981 tgcgaaaacg acacgttaca acgctgggtg gctcgggatt gcagggtgtt ccggagacct
    26041 ggcggcagta taggctgttc acaaaatcat tacaattaac ctacatatag tttgtcgggt
    26101 tttatcctga acagtgatcc aggtcacgat aacaacattt atttaatttt taatcatcta
    26161 atttgacaat cattcaacaa agttgttaca aacattgcca ggaaaagcat ataatgcgta
    26221 aaagttatga agtcggtatt tcacctaaga ttaacttatg taacagtgtg gaagtattga
    26281 ccaattcatt cgggacagtt attagtggta gacaagttta ataattcgga ttgctaagta
    26341 cttgattcgc catttattcg tcatcaatgg atcctttacc tgcaagcgcc cagagctctg
    26401 tacccaggtt ttcccctctt tcacagagcg gcgagccaaa taacaaacgg gtaaagccag
    26461 gttgatgtgc gaaggcaaat ttaagttccg gcagtcttac gcaataaggc gctaaggaga
    26521 ccttaaatgg ctgatacaaa agcaaaactc accctcaacg gggacacagc tgttgaactg
    26581 gatgtgctga aaggcacgct gggtcaagat gttattgata tccgtactct cggttcaaaa
    26641 ggtgtgttca cctttgaccc aggcttcact tcaaccgcat cctgcgaatc taaaattact
    26701 tttattgatg gtgatgaagg tattttgctg caccgcggtt tcccgatcga tcagctggcg
    26761 accgattcta actacctgga agtttgttac atcctgctga atggtgaaaa accgactcag
    26821 gaacagtatg acgaatttaa aactacggtg acccgtcata ccatgatcca cgagcagatt
    26881 acccgtctgt tccatgcttt ccgtcgcgac tcgcatccaa tggcagtcat gtgtggtatt
    26941 accggcgcgc tggcggcgtt ctatcacgac tcgctggatg ttaacaatcc tcgtcaccgt
    27001 gaaattgccg cgttccgcct gctgtcgaaa atgccgacca tggccgcgat gtgttacaag
    27061 tattccattg gtcagccatt tgtttatccg cgcaacgatc tctcctatgc cggtaacttc
    27121 ctgaatatga tgttctccac gccgtgcgaa ccgtatgaag ttaatccgat tctggaacgt
    27181 gctatggacc gtattctgat cctgcacgct gaccatgaac agaacgcctc tacctccacc
    27241 gtgcgtaccg ctggctcttc gggtgcgaac ccgtttgcct gtatcgcagc aggtattgct
    27301 tcactgtggg gacctgcgca cggtggtgct aacgaagcgg cgctgaaaat gctggaagaa
    27361 attagctccg ttaaacacat tccggaattt gttcgtcgtg cgaaagataa aaatgattct
    27421 ttccgcctga tgggcttcgg tcaccgcgtg tacaaaaatt acgacccgcg cgccaccgta
    27481 atgcgtgaaa cctgccatga agttctgaaa gagctgggca ccaaagatga cctgctggaa
    27541 gtggctatgg agctggaaaa catcgcgctg aacgacccgt actttatcga gaagaaactg
    27601 tacccgaacg tcgatttcta ctctggtatc atcctgaaag cgatgggtat tccgtcttcc
    27661 atgttcaccg tcattttcgc aatggcacgt accgttggct ggatcgccca ctggagcgaa
    27721 atgcacagtg acggtatgaa gattgcccgt ccgcgtcagc tgtatacagg atatgaaaaa
    27781 cgcgacttta aaagcgatat caagcgttaa tggttgattg ctaagttgta aatattttaa
    27841 cccgccgttc aaatggcggg ttaattttta tatgcccaaa cacaaaaaat tgtaaaaata
    27901 aaatccacta tcggacattc atatattcta aaaaaaatat atcggtataa attataaaca
    27961 aatcgatgat ttttaatcat ctatcttcct ctcagttaca tgaataaacc accaaatata
    28021 gcgcacccct ataatttcca atactttatt catcaaacat ttaattcata ccatcaatta
    28081 attactttta catttaataa aattaataat atttttaaga aaaactaaaa acattcgaca
    28141 atcaaataag atattcttgc accgatatac ttttttcttt tctgttaaag gactcagtta
    28201 tgtttaaagg acaaaaaaca ttggccgcac tggccgtatc tctgctgttc acagcacctg
    28261 tttatgcggc tgatgaagga tccggtgaaa ttcactttaa aggtgaagtt attgaagcac
    28321 cgtgtgaaat acatcaggat gatattgata aagaggttga actcggtcag gtgaccacca
    28381 gccacattaa tcagtcacat cacagcgatg ccgttgctgt cgacctgcgc ttagtcaact
    28441 gtgatctgga aaactccagc aacggttccg gtggcaagat ttctaaagtt gcagtcacct
    28501 ttgatagctc agcgaaaacc accggcgcag atccgattct caacaacacc agcacgggtg
    28561 aagccaccgg cgtcggcgta cgtttaatga ataaagatca aagcaatatc gttttaggca
    28621 ccgctactcc agatatcgac ctggctccga cctccagcga acaaacgctg aatttctttg
    28681 cctggatgga acaaattgat caggctacac ctgtaacgcc aggcgcagtt acggcgaatg
    28741 caacctacgt gctggattat aaataagtat tactttggcg gcgctatcgc cgcctttcat
    28801 aaaatattta tcaggtatgg acaccgtgaa tatttatcga ctctcttttg tatcctgcct
    28861 ggtcgtggcg atgccttgcg cattggcggt cgaattcaac cttaatgttc tcgataaatc
    28921 gatgcgcgac cgcattgata tttccttatt aaaggaaaaa ggagtcattg ctcccggtga
    28981 gtattttgtt agcgttgcgg taaataataa ccaaatcagt aacgggcaaa agattaactg
    29041 gcacaaaaat gacgataaaa ccattccgtg catcaatgat ttactggtcg ataaatttgg
    29101 cttaaaacct gaagtccgtc agtcgttacc attgataaat cagtgcgtcg attttagctc
    29161 ccgacctgaa atgctcttca atttcgatca agccaatcag caactgaata ttagtattcc
    29221 gcaagcctgg ctggcgtggc actcagaaaa ctggacccca ccctccacat ggaaagaagg
    29281 tgtcgccggt atcctgatgg attacaactt gtttgccagc agctaccgcc cacaggacgg
    29341 cagcagcagc actaacctga acgcctacgg taccaccgga attaacgccg gggcatggcg
    29401 cttacgtagt gattatcagt tgaatcagac tgatagcgat gataaccatg aacagtcagg
    29461 cggaatatcg cgcacctatc tttttcgtcc attaccgcaa ttaggctcta aattaaccct
    29521 cggcgaaacg gattttagtt ccaatatttt cgacggtttt tcttataccg gcgcggcact
    29581 ggcaagtgac gagcgaatgt tgccatggga actacgcggc tacgccccac aaattagcgg
    29641 tattgcacag accaatgcca cggtgacgat cagtcaatca ggccgcgtca tttaccagaa
    29701 aaaagtccca ccaggcccat ttatcattga cgaccttaat cagtctgttc agggcacact
    29761 ggatgtcaaa gtgacggaag aagatggtcg ggtgaacaat ttccaggttt cggcagcatc
    29821 gacgcccttc ctgactcgtc agggacaggt tcgctataaa ctggccgcgg gtcagccacg
    29881 gccctccatg tcacatcaaa ctgaaaatga aacctttttt agcaatgaag tttcctgggg
    29941 gatgctgtca aacacctcgc tgtacggcgg cctgctgctt tctggtgatg actaccattc
    30001 tgccgcaatg ggtattgggc aaaatatgct gtggcttggt gcgctgtcgt ttgatgtcac
    30061 gtgggccagt agccattttg atactcagca ggacgagcgg ggcttaagct accgttttaa
    30121 ttacagcaaa caagtggatg ccactaacag cacgatttcc ctcgccgctt atcgtttctc
    30181 cgatcgtcat tttcacagct acgccaacta tctggatcac aaatacaacg acagcgatgc
    30241 gcaggacgaa aaacagacga tcagcttatt gtgggccaac cgattacccc actaaacctc
    30301 aatctttacg ccaacctgct acatcaaacc tggtggaatg cagacgcctc cacgaccgcc
    30361 aacatcacgg caggttttaa tgttgatatt ggtgactgga gagatatctc tatttcgacg
    30421 tcattcaata caacccatta cgaagataaa gatcacgaca accagattta cctgtcgatt
    30481 tcgctcccct tcggtaacgg tggtcgggtt ggctatgaca tgcaaaacag tagccacagc
    30541 accacacacc gcatgtcgtg gaacgatacg ctggatgaac gtaatagctg gggcatgtct
    30601 gccggactgc aatccgaccg tcctgacaat ggagcccagg tgagcggtaa ctatcagcac
    30661 ctgagttcag cgggtgagtg ggatatttct ggtacctatg ccgccaatga ttacagttcc
    30721 gtcagcagca gctggagcgg ttctttcacc gcaacccaat atggtgcagc gtttcatcgc
    30781 cgcagctcca ccaatgaacc acgcctgatg gtcagcaccg atggcgtggc agatattccg
    30841 gttcagggca atctcgacta caccaaccat tttggcattg cggtggtgcc gttgatttcc
    30901 agttaccagc cttccaccgt ggcggtgaac atgaatgact tacccgacgg cgtaacagtt
    30961 gcagaaaacg ttatcaaaga aacgtggatt gaaggcgcga taggttacaa atcactggct
    31021 tcccgttccg gtaaagacgt taacgtcatc attcgcaacg ccagcggtca gtttcctccc
    31081 ctcggagcgg atatccgcca ggatgacagc ggcattagcg tggggatggt tggcgaggaa
    31141 ggacatgcct ggttaagcgg agtcgctgaa aatcaaaagt ttaccgtggt ctggggtgat
    31201 agccagcatt gctcgctcca tcttcctgaa catatggaag acaccgcaaa tcgcctgatt
    31261 ttaccttgtc attaattaag gaaatgaaaa tgacatttat gaaaggacta ccccttttgt
    31321 tgttggttgc cagtctgtgc agccacgctg cactacaacc cgatcgcact cgtattgtgt
    31381 ttaacgctaa tgataaagct accagtctgc gggtggataa tcgtagcgat aaactcccct
    31441 acctcgctta ttcctggctc gaaaatgaaa aaggggagaa aagcgatgat cttctggtcg
    31501 ccctgccgcc gatacaacgt ctggaaccga aagcaacaac gcaggtacgg atagtaaagc
    31561 aagcctcgac cacaaaatta ccgggcgatc gcgaaacgct atttttctac aatatgcgtg
    31621 aaattcctcc cgccccggaa aaaaacagcg accatgccgt tctgcaagtc gccattcaga
    31681 gtcgtatcaa ggtgttctgg cgaccggctg cattacgcaa gaaagcagga gaaaaggtcg
    31741 aactgcagtt acaggtcagc cagcagggca accaactgac actgaaaaat cctaccgcgt
    31801 attatctgac cattgcctac ctgggacgaa atgaaaaagg cgttctcccc ggcttcaaaa
    31861 ccgtgatggt tgcgcctttt agtacggtca acacaaacac cggaaattat agcggcagcc
    31921 agttttatct cggttatatg gatgattacg gcgcgttacg catgacgacg ctcaactgca
    31981 gcggacaatg ccatttacaa gccgtggagg cgaagaaatg agtgctggca agggagtgtt
    32041 gctcgtcatc tgtctgttat ttctgccgtt gaagtctgcg cttgccctga actgctactt
    32101 tggttcatcc ggtggctcgg tagaaaaatc agaagcgatt caaccttttg ctgtaccagg
    32161 caatgccaaa ctcggcgata agatttggga atctgacgat attaaaattc ccgtctattg
    32221 tgacaacaat accaacggca attttgaaag tgagcatgtt tatgcctggg taacccccta
    32281 cccgggggtg caggatcgct attatcaact cggcgtaact tacaatggcg ttgattatga
    32341 cgccaaccag ggaaaaagcc gcatcgacac taaccagtgt attgacagta agaacattga
    32401 catttatacc cctgagcaga ttatcgcgat gggatggcag aacaaaattt gctcgggtga
    32461 tcccgctaat attcacatgt cgcgtacctt tctcgctcgc atgcggttat acgtcaaaat
    32521 acgagaaatg ccgccgcatg attatcaaag tacgcttagc gactatatcg tcgtgcaatt
    32581 tgacggtgcc ggtagcgtta acgaagaccc tactgcccaa aacctgaaat atcatattac
    32641 tggtctggaa aacattcgcg tgctggattg cagcgtcaat ttttccattt caccggaaac
    32701 acaggtgatt gattttggta aatttaattt gctggatatt cgtcgccaca caatgtcgaa
    32761 aacattcagt attaagacga caaaatcaca aaatgaccaa tgcaccgacg gatttaaagt
    32821 cagttcctcc ttttataccg aagaaacgtt ggttgaagaa gataaagcat tgttgattgg
    32881 taatggtctg aagctacgtt tgctggatga aaacgcctcg ccttatacct tcaataaata
    32941 tgctgaatac gccgatttca ccagcgacat gttggtctac gaaaaaacct atacagctga
    33001 actttcgtcc attgcaggta cccccatcga ggctgggccc ttcgatacag tggtgctttt
    33061 taaaattaac tataactgat ttgccatgat gccctttgca gccggaaagg gcatctcttt
    33121 cagaaaattg ctgattttcc ccttttcctt gattgctgtt attttttcct ggcccggctt
    33181 tctttatgct gtatcgatac accaaagaga aaataatgag ggagcgaagg atgccagttt
    33241 tgcagtgggg aatgttatgt gtgctatcac ttctcctttc tattggtttc ctcgcggtac
    33301 atctcccggc ggcgctatta cttgggccga tgatcgccgg gatcatcttc agtatgcgcg
    33361 gaattactct gcaacttccc cgctccgctt ttctcgccgc gcaggccatt cttggctgca
    33421 tgattgcgca aaacctcact ggttcgattc tcaccacgct ggccgccaac tggccgatcg
    33481 tgcttgctat tttactggtg acgctgcttt ccagcgccat cgtgggttgg ttattggtgc
    33541 gctatagctc actgcctgga aataccggtg cctggggttc ttctccaggc ggcgcagcgg
    33601 caatggtcgc tatggcgcaa gattacggcg cagatattcg cctggttgca tttatgcaat
    33661 atctgcgagt gctgttcgtc gcgggggccg ctgttctggt cacccgcatg atgctgggtg
    33721 ataacgctga agcggttaac cagcaaatcg tctggttccc gccagtgagc attaatctcc
    33781 tgcttacgat tttgctcgca gtcgttgccg gtacggcggg atgcatgctg cgtcttcctt
    33841 ccggcacgat gctcatcccg atgctggcgg gcgcagtgct ccagtctggt cagctcatca
    33901 ccatcgaact accggaatgg ctgctggcga tggcgtatat ggcaattggc tggcggattg
    33961 gtcttggttt cgataaacaa atcttactgc gggcactacg cccgctaccg caaatcctgc
    34021 tgtcgatttt tgctctgctg gctatttgtg cgggtatggc gtgggggctg acccggttta
    34081 tgcacattga ttttatgact gcctacctcg ccaccagtcc cggcgggctt gataccgtgg
    34141 cggtcatcgc cgcagggagc aatgccgata tggcgcttat tatggcgatg caaaccctgc
    34201 gcctgttcag tattttgctg acggggccag ccattgcgcg gtttatttca acctatgcgc
    34261 cgaagcggtc ggcctagtgc tggcagccag ggcaccagta aaacgggcga gatgacagcg
    34321 tggttttctc aatgatgctg ccacagcgtt cgcacggttc gccatcacga tgaaaaacct
    34381 taaagcgaaa cagcgcccca tgatgcttat tctcatccac ctgccccctc gtagcgtagg
    34441 aaaatcgagg aatatccagt aacgcgtgtg cgagtgcatc cagttgcgcc gcattgagat
    34501 ctttcgcttt atgatttcca gtcaacccaa cctgccagag gatctccacc cgcaaataat
    34561 tgccaagccc agccagaaac gcctgatcga gcagtaatcc tgcaaactga cggttacgaa
    34621 agcgcggtga caataaccgt tcctttacca cctccggcgt cagattcgga tccagcacat
    34681 cgggaccaac gcgttgtaaa aacggatgcg tggtcagttg ttccggcctc aacatctcaa
    34741 tatccgaggc gctataaagc agaatggttt tgtcagccgt ttgcagtttt acccgcaata
    34801 ctcgcgtggt ctgcggctct tcgccggtat caaccacgcg ccagacgccg taaagctgat
    34861 tatggctgta gagcgttaag tcgttggaaa aatgagttaa caacgcctta ccacgcgttt
    34921 ccacatgggt aacgtgttga ccgataagtt gtgattgata agtttttaac tgcgggaagg
    34981 caaaccagac atcagttagt ggtttgcctt tgatcgccgc ctccaggtta tccgctgcac
    35041 ggcggatctc cgggccttca ggcatggtga tatccttttt tcattcttta ttaatcgggg
    35101 ttatgctgcg acaacaatct ccttttcggc aaatgtagag cgtagtcggc gggcgaacgc
    35161 cagtgcgtgc tcgccgtcgc catgcaggca gactgtttgc gccgtgaccg ttgcccattc
    35221 gccggtgata cttttgactc tgccgtgttg caccatctct agcgtttgcg ccaacgcctg
    35281 ttcttcgttt tcaatcaacg cgcctcgctg acttcgcggc accagcgagc cgtcagcctg
    35341 ataaccgcga tcggcaaaca cttcctcgcg cgttgtcagg ccatattgct tgcctgcacg
    35401 aatcagctcg cttcctgcca gcccaacgag aatcaatgcc ggatcgcaag cgtataccgc
    35461 tctggcgatg gcgtctgcca gttgtgcttc tttcgccgcc tggttgtaca acatgccgtg
    35521 cggtttgaca tgacgcatta cgccgccttg cgcacgggca atcgttgcca gcgcgccaat
    35581 ttgatacagc gtctgggcgt aaacggtttc tggcggcagc tgcatggcgc tgcgaccaaa
    35641 attttccctg tcgggaaaac tcgggtgagc gccaatcgcg acaccatttt ttattgcttc
    35701 acgcacgcaa gcctgcatga tttgcgcatc gcctgcatga aatccacagg caatattggc
    35761 agaggaaacc agcgttaata gctctgcgtc gctggcgcag ccttcgccca gatcggcgtt
    35821 caggtcaatt ttcattgtgc agccgccaca ctaattgttc gaaataacgt tgctgatctt
    35881 gccgcgcttt cagtgcctct tccagtgaac actggacaaa atgaatcggc tgaccgagcg
    35941 gaatttgcgc cagatggtac atatcagcct caatgataca ggcaatacgc gggtaaccat
    36001 cggtggtctg tgcgtcgttc atcaacacaa tcggctgccc gttatgtggc acctgcacca
    36061 cgcccggtaa caaaccgtga gataacagtt cgcgatcggt ggtgcgtttt aaaatttgcc
    36121 cctgtaagcg atagcccatg cggttacttt gcgagctaag ctgccagggc gaacgccaga
    36181 atgcatcctg cgaggcgcga tcgaactcat gatattccgg ccccggcaag gcgcgaatgc
    36241 ggttgcccca cagcagctgt ttaacgccct gcgcttccat aaaatcacgc ttcgatttgc
    36301 caatcgggag tcggtcacca tccttcagta aacggccttc cagcccgcca atccccactt
    36361 tgagatcggt gctgcatgac cccattaccg gcggaacatc aataccaccc gcgaccgcca
    36421 gataactgcg catcccgtgc tgcgggcgtt taagcgttaa acgctggcct gctttcatcg
    36481 gcaatcgcca gccggtccag acggcattat catccagccg cgcttcgcaa ccggcacccg
    36541 tcagagcaaa ccacccatca gtttcgaact caaccgttaa ctgaccgagc gtgatctcca
    36601 gcgcgggggc attggcgtca ttacccacca gcaggttagc aatgcgtaac gcaggcatat
    36661 ccagtgcgcc gcagtggctg attcccgact ggcgaaaacc gtgacgaccg ccatcctgca
    36721 cagtggtata catgcccgca cgaataatct tcagcatact ccctccttct gcggtacaaa
    36781 gcgcacgctg tctcccggac gtaataagat gggttcgtca cgcgccggat caaacaggct
    36841 gagtgaggta tgaccaatca actgccagcc acccggcgtt gccagcggat aaacaccagt
    36901 ctgcggcccg ccgatcccga cagaacctgc cggaacgagc aagcgcggtt cagcgcgccg
    36961 tggcgtgtgt agttgttccg gcaaactccc gagatacggg aagcccggtt gaaaacctaa
    37021 aaaccagacc acgtattcca cggaggagtg caattcaaca acctgttttt cgctaaaccc
    37081 gcaatgcgcc gcgaccaccg ccaaatccgg tccgcctgca ccaccgtaaa ccaccggaat
    37141 ttcaataaag cgagactcag gttccagggc ctcgctctcc tcccaccagc gttgcaaacg
    37201 ctctatggca tccagcgcca gcgactcagg attacgcaaa atcaccgtga tattgttcat
    37261 gccgggaatg gcttcaacca cattcggcat atccaccaga cgctgcgcca gtcgccagat
    37321 ccgtttctgg ctagccagcg tcaccggcgg ttccagttcc agcactaccg ccgtttcacc
    37381 tatcagataa caacgcgctc gttgcacttt cgatccctct gattattcgt tatgcaggat
    37441 taggaatatc aataaaggtc acatcaagat cggtattttc attcagccac tcgctcaatg
    37501 cgcgaatacc accacgttca gtggcatggt gacctgcagc ataaaaatgc aacccctgct
    37561 cgcgggctga atgaatggtc tgttcagaaa cttcgccagt aataaaagca tccacgccaa
    37621 aacgcgcggc gctatcgata aaactttgcc cgccgcccgt gcaccaggcg acgcgctgga
    37681 ccacctcagg tccggtatcg ccacaccata acggcttacg tcccagacgc gcttcaatcc
    37741 aggaggctag ttccagtccc ggcacaggca tggtcagctc tccccacggc accaacggct
    37801 caatttcgcc catgaccgtg atccccagta acgccgccag ctgtgcgtta ttgcccagct
    37861 caggatgtac gtcaagcggc aaatgccagc catacaggtt gatatcattc gccagcaacg
    37921 tttttaaacg gttacgcttc attccgcgaa tgaccggaga ctctcctttc cagaagtagc
    37981 catgatgcac aatgaccgca tccgcgccca gacgcactgc ctcatcgagc aaagcctggc
    38041 tggcggtgac accggtaaca attttttgca ccgtctcttt gccttctacc tgcaaaccat
    38101 tcggcgcgta atcactaatc gccgcgctgt tcagtttttc gttaatcagt tgttccagtt
    38161 cggtgttttt catcttcgcg ccttccgtat tctgatagct acaccgttaa catagcgtta
    38221 cctcgcctgt tcgtcgatag ccaatctttt gacatattaa gaaacaaacc cgacgccaaa
    38281 agacgcaaat tgtttatatc atcacgcttc agctaatgta tatttatgca atcagacagc
    38341 atattgcata aataatcaga tctgtatcaa agttttaact ttaaggaaac acaagcacgc
    38401 cgcgcaacta ttacgtgacg ttgcagtcaa ttgtggaatt attatgaata aacacgcatc
    38461 acaaccgcgc actatttact atgtcgttgc gctgcaaatc tgggaatatt ttagctttta
    38521 cggcatgcgt gccctgctga ttctctatct caccaatcaa ctaaaataca acgatactca
    38581 cgcctacgag ttatttagcg cctactgttc gctggtgtat gtcacgccaa tcctcggtgg
    38641 ctttttggcg gataaagttc tcggcaatcg catggcggtg atgctggggg cgttgttgat
    38701 ggcgatcggt catgtggtgc tgggtgccag tgagatccat ccgtcattcc tctatctgtc
    38761 cctggcgatt atcgtttgtg gctatggtct gtttaaatcc aatgtcagtt gcctgctcgg
    38821 cgagctgtat gagccaaccg atccgcgtcg tgatggcggt ttctcgctga tgtatgcagc
    38881 gggtaacgtg gggtctatta tcgcacctat cgcctgtggt tacgcccagg aagagtacag
    38941 ctgggcgatg ggctttggcc tggcggcggt gggtatgatc gcgggtctgg tcattttctt
    39001 atgtggcaat cgtcatttca cacatacccg cggcgttaat aaaaaagtac tgcgtgcaac
    39061 aaactttctc ctgcctaact ggggatggct gctggttctg ctggtggcaa cgcctgcgct
    39121 gattaccgta ctgttctgga aagagtggtc ggtatacgcc ttaattgtcg cgactattat
    39181 tggcctgggt gtactggcaa aaatttatcg caaagcagaa aaccagaaac agcgtaagga
    39241 gctggggctg attgtgacgc tcaccttctt cagtatgttg ttctgggcct tcgcacaaca
    39301 gggcggcagc tcaattagcc tttatatcga ccgcttcgtt aaccgcgata tgtttggtta
    39361 taccgttccg actgctatgt tccagtcgat taatgccttc gcagttatgc tatgcggtgt
    39421 gttcctggcg tgggtggtta aagaaagcgt cgcaggtaat cgtaccgtgc gcatctgggg
    39481 aaaatttgct cttggccttg gcctgatgag cgccggattc tgcattctga ccttaagcgc
    39541 ccgctggtcc gcaatgtatg gtcactcttc tctgccactg atggtgttag gcctcgcggt
    39601 gatgggcttt gcggaactgt ttatcgaccc ggttgccatg tcgcaaatta cgcgcattga
    39661 aattccgggc gtgaccggcg tattaaccgg catctacatg ctgctttctg gcgcgatcgc
    39721 gaactatctg gcgggcgtga ttgccgatca gacatcgcag gcttcgtttg atgcttccgg
    39781 ggcgatcaac tactccatca atgcatatat tgaagtattt gatcaaatta cctggggcgc
    39841 actggcgtgt gtaggactgg tactgatgat ttggctgtat caggcgctga aattcagaaa
    39901 ccgcgcgctg gcgctggagt cataataggc cagcaccgat gcctgatgcg acgcttgacg
    39961 cgtcttatc
//
LOCUS       DAESHI010000029        39833 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.029, whole genome shotgun sequence.
ACCESSION   DAESHI010000029 DAESHI010000000
VERSION     DAESHI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39833
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.029"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            4..435
                     /locus_tag="KW011_001545"
     CDS             4..435
                     /locus_tag="KW011_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HBH7659908.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQFA
                     VQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            518..1420
                     /locus_tag="KW011_001546"
     CDS             518..1420
                     /locus_tag="KW011_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HBH7659909.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="KW011_001547"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="KW011_001547"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HBH7659910.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="KW011_001548"
     CDS             complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="KW011_001548"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HBH7659911.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="KW011_001549"
     CDS             complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="KW011_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBH7659912.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENV
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5330..6037)
                     /locus_tag="KW011_001550"
     CDS             complement(5330..6037)
                     /locus_tag="KW011_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HBH7659913.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="KW011_001551"
     CDS             complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="KW011_001551"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBH7659914.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6895..7098)
                     /locus_tag="KW011_001552"
     CDS             complement(6895..7098)
                     /locus_tag="KW011_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBH7659915.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            7652..7972
                     /gene="cyaY"
                     /locus_tag="KW011_001553"
     CDS             7652..7972
                     /gene="cyaY"
                     /locus_tag="KW011_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HBH7659916.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8012..10558)
                     /gene="cyaA"
                     /locus_tag="KW011_001554"
     CDS             complement(8012..10558)
                     /gene="cyaA"
                     /locus_tag="KW011_001554"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HBH7659917.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            10945..11886
                     /gene="hemC"
                     /locus_tag="KW011_001555"
     CDS             10945..11886
                     /gene="hemC"
                     /locus_tag="KW011_001555"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBH7659918.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            11883..12623
                     /gene="hemD"
                     /locus_tag="KW011_001556"
     CDS             11883..12623
                     /gene="hemD"
                     /locus_tag="KW011_001556"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HBH7659919.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            12645..13856
                     /gene="hemX"
                     /locus_tag="KW011_001557"
     CDS             12645..13856
                     /gene="hemX"
                     /locus_tag="KW011_001557"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBH7659920.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     GDTPAAAPQGE"
     gene            13859..15055
                     /gene="hemY"
                     /locus_tag="KW011_001558"
     CDS             13859..15055
                     /gene="hemY"
                     /locus_tag="KW011_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HBH7659921.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            15717..15878
                     /locus_tag="KW011_001559"
     CDS             15717..15878
                     /locus_tag="KW011_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659922.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(15962..17197)
                     /locus_tag="KW011_001560"
     CDS             complement(15962..17197)
                     /locus_tag="KW011_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBH7659923.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(17344..17420)
                     /locus_tag="KW011_001561"
     tRNA            complement(17344..17420)
                     /locus_tag="KW011_001561"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17384..17386),aa:Pro,seq:tgg)
     gene            complement(17463..17549)
                     /locus_tag="KW011_001562"
     tRNA            complement(17463..17549)
                     /locus_tag="KW011_001562"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17513..17515),aa:Leu,seq:cag)
     gene            complement(17570..17645)
                     /locus_tag="KW011_001563"
     tRNA            complement(17570..17645)
                     /locus_tag="KW011_001563"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17610..17612),aa:His,seq:gtg)
     gene            complement(17704..17780)
                     /locus_tag="KW011_001564"
     tRNA            complement(17704..17780)
                     /locus_tag="KW011_001564"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17744..17746),aa:Arg,seq:ccg)
     gene            complement(17883..19268)
                     /locus_tag="KW011_001565"
     CDS             complement(17883..19268)
                     /locus_tag="KW011_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH7659924.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(19459..20199)
                     /gene="rffM"
                     /locus_tag="KW011_001566"
     CDS             complement(19459..20199)
                     /gene="rffM"
                     /locus_tag="KW011_001566"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HBH7659925.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(20202..21554)
                     /gene="wzyE"
                     /locus_tag="KW011_001567"
     CDS             complement(20202..21554)
                     /gene="wzyE"
                     /locus_tag="KW011_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HBH7659926.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWMALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLIIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(21551..22630)
                     /gene="rffT"
                     /locus_tag="KW011_001568"
                     /gene_synonym="wecF"
     CDS             complement(21551..22630)
                     /gene="rffT"
                     /locus_tag="KW011_001568"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HBH7659927.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(22627..23877)
                     /gene="wzxE"
                     /locus_tag="KW011_001569"
     CDS             complement(22627..23877)
                     /gene="wzxE"
                     /locus_tag="KW011_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HBH7659928.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(23879..25009)
                     /gene="rffA"
                     /locus_tag="KW011_001570"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(23879..25009)
                     /gene="rffA"
                     /locus_tag="KW011_001570"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HBH7659929.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(25014..25688)
                     /gene="rffC"
                     /locus_tag="KW011_001571"
                     /gene_synonym="wecD"
     CDS             complement(25014..25688)
                     /gene="rffC"
                     /locus_tag="KW011_001571"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HBH7659930.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(25666..26547)
                     /gene="rfbA"
                     /locus_tag="KW011_001572"
     CDS             complement(25666..26547)
                     /gene="rfbA"
                     /locus_tag="KW011_001572"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBH7659931.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(26566..27633)
                     /gene="rffG"
                     /locus_tag="KW011_001573"
     CDS             complement(26566..27633)
                     /gene="rffG"
                     /locus_tag="KW011_001573"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBH7659932.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(27633..28895)
                     /gene="wecC"
                     /locus_tag="KW011_001574"
     CDS             complement(27633..28895)
                     /gene="wecC"
                     /locus_tag="KW011_001574"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HBH7659933.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(28892..30022)
                     /gene="wecB"
                     /locus_tag="KW011_001575"
     CDS             complement(28892..30022)
                     /gene="wecB"
                     /locus_tag="KW011_001575"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBH7659934.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(30078..31124)
                     /gene="wzzE"
                     /locus_tag="KW011_001576"
     CDS             complement(30078..31124)
                     /gene="wzzE"
                     /locus_tag="KW011_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HBH7659935.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(31136..32239)
                     /gene="wecA"
                     /locus_tag="KW011_001577"
     CDS             complement(31136..32239)
                     /gene="wecA"
                     /locus_tag="KW011_001577"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HBH7659936.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(32479..33738)
                     /gene="rho"
                     /locus_tag="KW011_001578"
     CDS             complement(32479..33738)
                     /gene="rho"
                     /locus_tag="KW011_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBH7659937.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(33823..33924)
                     /gene="rhoL"
                     /locus_tag="KW011_001579"
     CDS             complement(33823..33924)
                     /gene="rhoL"
                     /locus_tag="KW011_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HBH7659938.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(34066..34395)
                     /gene="trxA"
                     /locus_tag="KW011_001580"
     CDS             complement(34066..34395)
                     /gene="trxA"
                     /locus_tag="KW011_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HBH7659939.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            34526..35791
                     /gene="rhlB"
                     /locus_tag="KW011_001581"
     CDS             34526..35791
                     /gene="rhlB"
                     /locus_tag="KW011_001581"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HBH7659940.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            35925..37409
                     /gene="gppA"
                     /locus_tag="KW011_001582"
     CDS             35925..37409
                     /gene="gppA"
                     /locus_tag="KW011_001582"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HBH7659941.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(37456..39477)
                     /gene="rep"
                     /locus_tag="KW011_001583"
     CDS             complement(37456..39477)
                     /gene="rep"
                     /locus_tag="KW011_001583"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HBH7659942.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
BASE COUNT         9866 a        10828 c        10049 g         9090 t
ORIGIN      
        1 aaggtggcgt taaatctcgc ttcaggtgaa gggaatttct ttatccgtcc tggcggcgtg
       61 ttttatgtcg cgggagataa agtcgacatc gttcgtctgg atgccttcaa aaccagtaaa
      121 gagattcagt ttgcggtgca gtcagggcca atgttgatgg aaaacagtgt aattaatccg
      181 cgtattcatc ccaacgtcgc ctcacgtaaa attcgtaacg gtgttgggat taataaacat
      241 gggaatgccg tgtttttgtt gagccagcag gcgacaaatt tttatgattt tgcctgttat
      301 gccaaagcga aactgaacgt tgagcagctg ctttatcttg atggcacgat ttcacatatg
      361 tatatgaaag gcggggcaat cccgtggcaa cgctatcctt ttgtcaccat gatttccgtc
      421 gagaggaaag gttaagcatt gtacactcga ggaggccaga caaactgggg ctggctacac
      481 agctgaaaaa tcatcagata aatgaaggga aacaaaaatg aaaaaaacgc tcgcaacgtt
      541 atttttatta acgtgtctgg gatcatcatc cgcttatgcg gataacgctt tgattttaca
      601 aactgatttt agcctgaaag atggtgccgt ctcggcgatg aaaggggtcg cattcggtgt
      661 tgaccataat cttaaaatat ttgatttaac acacgaaatt ccaccgtata acatctggga
      721 aggcgcttac cgcctgtatc agaccgccag ttactggccg caaggttcgg tatttgtcag
      781 tgtggtcgat cctggtgttg gtacagaccg taaatcggta gtgctaaaaa ccaaaaacgg
      841 tcaatacttc gtttcaccag ataacggtac gctcactttg gtggcggaat cgctgggtat
      901 tgagagcgtc cggcaaatcg atgaaaaaac caaccgcctg aaaggttccg aaaagtcata
      961 taccttccac ggtcgtgacg tgtatgccta caccggtgca cgcctggctt ccggagcgat
     1021 cacgttcgaa caggtggggc cagagcttcc tgcaaaagtt gtcgaacttt cttatcagaa
     1081 agccaaagca acaaaaggtg aagtgaaggg caatatcccg attctcgata ttcagtacgg
     1141 caacgtctgg agcaatatca gcgacgagtt gctgaatcag gcaggaatca aactcaacga
     1201 cacgttgtgt gtaaccattt ccgaaggttc ccagcaaaaa tacgcaggga aaatgccgta
     1261 tgtcgcaagc ttcggcgatg tgccggaagg ccagccgatg gtgtatctaa acagcttatt
     1321 gaatgtttcc gtggcgctca atatggataa tttcgcgcag aaacatcaag ttgcgtctgg
     1381 tgctgactgg aatattgatg tgaagaagtg cgctaagtaa ggtgctgatg caggatgcgg
     1441 tgtggcgcac cgcatcctgc aacggagttg taaattacac cgtctccagc cgggcgtaag
     1501 ccgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg ctatgttcac
     1561 cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgc acgcgctggc
     1621 cgagtttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga tggctaaccg
     1681 ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc agctcgccga
     1741 taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt tccgcgtagg
     1801 tcagcgtcag tttctgcatc gcacgggtta cgccaacgta ggccagacgg cgttcttctt
     1861 ccagacgtcc gccttcatcc agcgacatct ggcttgggaa catgccctct tccataccga
     1921 cgataaacac ctgcgggaac tccagccctt tcgccgagtg tagcgtcatc aactgcaccg
     1981 catcctgcca agtatccgcc tgcccttcac ctgcttccag tgccgcatgg gagaggaacg
     2041 cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc gttgccgtca
     2101 ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc tgctcgtaca
     2161 tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc ggcatatcgg
     2221 cagtttcttg cgctaaggca tcgattaatt ccataaatcg ctgcaaggcg ctggcagctc
     2281 gcccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt gttaactggc
     2341 gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc cgcgttggcg
     2401 tattcaccac gcgctcaaac gccgcgtcgt cgttacggtt ggcaatcaga cgcagatacg
     2461 agagcgcgtc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa atacggtacg
     2521 gcatactggc ctgcaacagt gcttcttcca gtacacgcga ctgggcgtta ctgcggtaga
     2581 gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca ggttttgatg cggttaacca
     2641 caaaacgcgc ttcgtcgagt tcgttaaaag cgcaatagag ggaaataggc tcaccgtccg
     2701 cgccatcggt ccacagtttt ttacccagac gcccgttgtt gttttcaatc agggcgttcg
     2761 cggcgctcag aatattgctg gtagagcggt agttctgctc cagacggata gtttcagcac
     2821 cggggaaatc attgaggaaa cgctgaatat tctctacctg cgccccgcgc cagccgtaga
     2881 ttgactggtc gtcatcgcca acgatcatca ctttgccggt atcgcccgcc agcaggcgga
     2941 tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggatg ttggtaaaac
     3001 gttcgcggta gtgttgcagg atatgcggct tgttaagcca caactcgtga gcgcgcagca
     3061 gcagctcggc gaagtccacc aggcccgcac gatcgcacgc ttcctgatac gcctgataca
     3121 ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc ggacgcaggc
     3181 cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac tgcttctcgt
     3241 cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttca ctgtcgagga
     3301 tctggaaatc ctgcggtaga ttggcgtcca tatggtgcgc acgcagcaaa cggtgcgcca
     3361 gcccgtggaa ggtgccgacc cacatgccgc cctggctggt gcccatcagt tgcccgatac
     3421 gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc gagtatggcg
     3481 agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc gtcttaccac
     3541 tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct tcgcgctgtt
     3601 tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg taaaaatagg
     3661 ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca gatttactga
     3721 ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgcggca gtaaacggct
     3781 gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct gcattccgct
     3841 gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga tctcgccgat
     3901 cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt ttgagcgccc
     3961 gtgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca gttccggctg
     4021 ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt ttaaggtgtc
     4081 gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa tcattgctgc
     4141 gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg cttgttcaat
     4201 cgaacgaaaa cgccaacgcg tcacgtcgtg ataaatctcg ggttccgctt cccgtaccgc
     4261 ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat gataattttg
     4321 cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat cgtaaagggt
     4381 atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa aacgcattat
     4441 ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg ttgaaaatca
     4501 agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagcagctc ctgcacacca
     4561 cgaagatcgc cgctcgactc cagcatatgc gtggcgaacg agtgacgtaa tttatgcgga
     4621 tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt ctgcacatta
     4681 cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc gctaccaaac
     4741 aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc aatcggcagg
     4801 cggcgctctt tgctgccttt ccccattacc cacacttcac cagactccag gtcgaggtgt
     4861 ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat cacttccagc
     4921 attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tgtccagcag ccgattcata
     4981 tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga aacacctttc
     5041 gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct acgtagcgca
     5101 gaaagccgta acgcgaggct tgctgctccc agccctttac ggcgactgcg tacagcaaaa
     5161 ttgcgcacca tcgtcacatc acattgctgc cagctttgca ggacgttttc gctggcaaaa
     5221 ttgatgatcg cctcaagctg acgctggtag ttaagcaggg ttatcgggct aagctggcgc
     5281 tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt catacgcgtt
     5341 caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt aactgcgttc
     5401 cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacgaca cccaaatcag
     5461 catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc ggtagcacca
     5521 ccagcagctc tggtccgtta agcggcccaa gatagtgctg ttcctgcccc aaacgctgaa
     5581 tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc gacggcgcac
     5641 ctaagcgcca gcgatccgga aacaggcgca gactcgcacc tgccaggccg agatcgcgcg
     5701 cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg agactgcgct
     5761 gcaggtagag taggcgataa aacaggcctt cgttggcgat agcctgttcc atcaacagcg
     5821 ccatgttctc ttccagaaca tgaatatgat tacgtgcgcg ggccatgtgc cactcgacca
     5881 acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg cgcgcattac
     5941 ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca tcaagctccg
     6001 tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc cgtcgtagac
     6061 atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg cgatatcaag
     6121 acgaccgccg gggagttcca cgcgtacttc ttcggccagc aaaccttgct gaatccctac
     6181 tgcaaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc cacgctcata
     6241 aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat tggcgcgctc
     6301 cggaaaacgc tcgtggcttt ccagaacagg accaagcgtt tctaccgccg cggtatcgac
     6361 atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc ataagattgt
     6421 ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa acggcacggc
     6481 ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat cggtgacggt
     6541 cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg tcagtccttt
     6601 cagacgcaca aaacgggcaa agcagcgcgc accgttgccg cactgcgcca cttcactgcc
     6661 atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg gctcaaccac
     6721 cagcagttgg tcaaacccta cccccaagtg ccgatcagcc aggcgacgaa tcagctccgg
     6781 tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc caaggccatg
     6841 cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg acttttagta
     6901 attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca ccgtggattg
     6961 cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag gcgggaaata
     7021 gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata cagtgagtgc
     7081 cttaaacacg tttttcatta tggattgcct gtaaagttca tcgctttctg ctttctgctt
     7141 tctgctttct gctttctgct ttctgctttc tgctttctgc tttctgcttt ctgctttctg
     7201 ctttctgctt tctgctttct gctttctatc atcgcaggag aagacgtaaa agcaagcgtt
     7261 tagcgactct ttatgaagtc tgaaaagtga ggaaatgctg cgagtataac gcttactcgt
     7321 ggggatttat gttttgctgg tagtttcgtg atcggcacct cgcgccagga tggctctatg
     7381 cactatcgca atgtggtttg tttgttgtct tttttagttc cgtcagcgtg aggatgctcg
     7441 ctcggtaaac ctgatcataa tgtttaccag aaacagggaa aggcgtggtg taactgcgcc
     7501 ctaatgaaga gacaaatctt tcgctacaca gagacatcat tattccggtg acggatgaat
     7561 cgtgccggaa cgcagccgca gaggtgcaaa ctttgaagcc ctgaccccat gccgctatac
     7621 tgccgccatc acttaaaaac aggatacaac aatgaacgac agtgaatttc atcgcctggc
     7681 tgatcaactc tggctgacca ttgaagaacg cctggacgac tgggatggcg acagcgatat
     7741 cgactgcgaa atcaacggcg gcgtgctgac cattaccttt gaaaacggca gcaaaatcat
     7801 tatcaaccgc caggaaccgc tgcaccaggt atggctggca accaaacaag gcggctacca
     7861 ttttgatctg aaaggcgatg agtggatttg cgatcgcagc ggcgaaacct tctgggattt
     7921 gctggaacag gcggcgacgc agcaggcggg tgaaacagtc agtttccgct aagattgcat
     7981 gccggataag cctcgctttc cggcacgttc atcacgaaaa atattgctgt aatagcggcg
     8041 tatcgtgatc ctgattggca ggcggcatgt taccgataga ttttgtgcgg aacggaatca
     8101 cctgttcacg accatcaacc ttcacaatct gatagaactg cggcaggttg aagttgatga
     8161 agcttgagcc gtaggtgaag cggtcgtgcg atgacgagta gaagcgactg acgtcacgca
     8221 ccagttcctc tttgctgcct tcgcagtggt gatacacctc aacccggttg ctttcgtcaa
     8281 gaatgtagat attaaagcca ttctcgtctt gcgtttcttc gaagaagaac tggatgatcc
     8341 cttcactagc aaagccgtcc accaccgccg gtaatttgac gtgattggtt tcaacctgca
     8401 ctgacaggcc gtgcagtttg ttatgcgaaa tagcgccata aaactcgatg gcgttctcca
     8461 gcttctgtac cgatacattc aggcgttcga agaacaagcc ccaggtttga ccagaaacgc
     8521 gcagcgcctt gaaacgcccg gtttcctggc gggtgctgga aaggcgcagt tcaatacact
     8581 cagaaaccag ttgctgcacg cgagtacgga ttaacccgcg cagatgctgg ctataacaga
     8641 agacttccac gctatctggc ggtgcggcgt cctgatgcat tttgccgaga atagttttca
     8701 gggcttcgat catcgattgc tcgccgttga agtgcagcgt acgcacttcg ttccacgagt
     8761 tgcggtacag caggtcaacg ctacctacca ggcaattttg attctcgcca aagctgaaga
     8821 catccagctt acggaaatca aaatgcacca cctgattgcg gaacgccgct gtcgggtcat
     8881 attccaggtt aacgataatc gccagatggc ggatctcaca cgggctgtag agcgccttcg
     8941 gtgtcggtgc aggtaagcgc agcgggaaat ggtgcgacac gtcggcgacc atctcctgca
     9001 acttaggcaa atcgacaatg ccgttacctt taatatacaa acgggtgcgc gaggtcagca
     9061 gaccgttaaa ccatgcccac gccaccagtt tattcaggta acggttatat tccagcggct
     9121 gatggctgat gatcgactca atatttggcg cgcggttata cagataccaa cctgaacggt
     9181 tagcccggcc cggcggcaca taaataaagg tcagattcgg ttccgagaga tcgggtgaaa
     9241 tctgcgggtt taccagcgtc actttacctg gtaatgcttc aaacgcggca tacagcttac
     9301 gcgtcagcac gccgatatcc tgcggactgg cggagacgct aaggttattg cgacgcgcaa
     9361 agcggatcag attacggtag ctctgcatca tcgcgtcgag caactcgttg tgcgcctcac
     9421 gcacctgatc aatcttccag ttagcacggt tatcgagcat tgccagacga gcttcgtccc
     9481 aaccccactc gctcactaac tggctcaaca ctgcgcgacg ccagcctaca caggcgcgtt
     9541 cacggctgag tttttcgcac acttttaaat agaagcagcg acgtactaaa tccagacggg
     9601 tgaaatcttc aatcgccgtc aggtattcag taacacgctc cagcatcatg cagtatggat
     9661 cgaggccaaa cgatacaatc tcgccgtcgt gcaaacgctg tttgatatct ttcgccagca
     9721 gacgtgggtt cgggtattcc caggaatagg cttccagcag cagtgttttc agcaccgctt
     9781 tgtatgggga atcgatactc ttgtagagct gccaaaggct ggcaccaaag tactcttcag
     9841 cagaaagcga gcttaagcca ccgagatcca gccattcatt tggcgtcagc acgccctgcg
     9901 cgtaaagcgt catcacatag tcgtcgtaat gctcttcttc gtcgcacggc accatattcc
     9961 acagaatacg cttaccggcg agacgcacgg cggtacgata aaattcgtca agcagcagta
    10021 tatgctgggt ggagccacaa tcttcgcccc ccaggctgcc gctttcatta tgacgaaagc
    10081 ggttttcatc aatcaggaag aagctgactt ccacacccag cgaggcggcc cagctttcca
    10141 gcaggctgca tttacgttgt agtaactggc gctcttcgct atcgagccag gattgatgac
    10201 agacccagat atccaggtca gaggaacaac tttgccctac ggacgaggtg ctgcccatgg
    10261 tgtatacacc agtaattgga agctcacctt tcggtggatc ctgtactgac attccacgat
    10321 acagttcaag ctcgttcagg tagtggcgtt gagtttcatc aggcgtgtaa aggcaaatgc
    10381 ctttgggaac gttaccatca aggtaacccg gcattagcgg atggtgatag tgcaacaatg
    10441 tcggcagtag actgtagacc tgttggaatg caggccccat agcagcaagc gcgcgatcca
    10501 cacgcaattg atttatggca tccagtctct gtttcagagt ctcaatatag aggtacaaga
    10561 cgtatcgcct gatttgctac ccgtcatgac tgtgattccg ccaacatcaa cggtaacacg
    10621 cggcattcgg gatatttcgt atgtcaaagg taaccgttac cacttttcgc gcctggtttt
    10681 tttagtttca cgacgaaaaa atggtctaaa acgtgatcaa tttaacacct tgctgattga
    10741 ccgtaaagaa agatgcgcta catacaagtg tagcaccgtt tattctctgt aaattcctta
    10801 ttacgacagc gtgaaacgtc tgtcaggatc cactgccaga cctcatttta cggtttgcgc
    10861 aggcgtctac gtttcaccac aacactgaca tcactctggc aaggatgtta ggatggacca
    10921 cggatgataa tgacggtaac aagcatgtta gacaatgttt taagaattgc cacacgccaa
    10981 agcccacttg cactctggca ggcacactat gtcaaagaca agttgatggc gagccatccg
    11041 ggcctggtcg ttgaactggt accgatggtg acgcgcggcg atgtgattct tgatacgccg
    11101 ctggcgaaag taggcggaaa aggcttattt gtaaaagagc tggaagtcgc gctcctcgaa
    11161 aatcgcgccg atatcgccgt acattcaatg aaagatgtgc cggttgaatt cccgcaaggt
    11221 ctgggactgg tcactatttg tgagcgtgaa gatcctcgcg atgcctttgt gtccaataac
    11281 tatgacagtc tggatgcgtt accagcaggc agtatcgtcg ggacgtccag tttacgtcgc
    11341 cagtgccaac tggctgaacg ccgcccggat ctgattatcc gctccctgcg cggcaacgtc
    11401 ggcactcgcc tgagcaaact agataacggc gaatacgatg ccatcattct tgccgtagcc
    11461 ggactaaaac gtttaggtct ggagtcccgc attcgcgccg cgttaccacc cgagatttct
    11521 cttccggcgg taggacaagg tgcagtcggt attgaatgcc gccttgatga tacgcgcact
    11581 cgcgagctgc ttgccgcgct gaatcaccac gaaactgcac tgcgcgttac cgcagaacgc
    11641 gccatgaata cccgtctcga aggcggatgt caggtgccaa ttggtagcta cgccgagctt
    11701 attgatggcg aaatctggct gcgtgcgctg gtcggcgcgc cggacggttc gcagattatt
    11761 cgcggtgaac gccgcggtgc gccgcaaaat gccaaacaaa tggggatttc gctggcagaa
    11821 gagctactga ataacggcgc gcgcgagatc ctcgctgaag tctataacgg agacgccccg
    11881 gcatgagtat ccttgtcacc cgcccgtctc ccgctggaga agagttagtg agccgtctgc
    11941 gcacactggg gcaggtggcc tggcattttc cgctgattga gttttctccg ggtcaacaat
    12001 taccgcaact tgctgatcaa ctggcagcgc tgggggagag cgatctgttg tttgccctct
    12061 cgcaacacgc ggttgctttt gcccaatcac agctacatca gcaagatcgt aaatggcccc
    12121 gactacctaa ttatttcgcc attggacgca ccaccgcact ggcactacat accgtaagcg
    12181 gacagaagat tctctacccg caggatcggg aaatcagcga agtcttgcta caattacctg
    12241 aattacaaaa tattgcgggc aaacgtgcgc tgatattacg tggcaatggc ggtcgtgagc
    12301 taattgggga taccctgacg gcgcgcggtg ctgaggtcac tttttgtgaa tgttatcaac
    12361 gatgcgcaat ccattacgat ggtgcagaag aagcgatgcg ctggcaatcc cgcgaggtga
    12421 cgacggtcgt tgttaccagc ggtgaaatgt tgcagcaact ctggtcgctg atcccacaat
    12481 ggtatcgtga gcactggtta ctacactgtc gactattggt cgtcagtgag cgtttggcga
    12541 aactcgcccg ggaactgggc tggcaagaca ttaaggtcgc cgataacgct gccaacgatg
    12601 cgcttttacg ggcattacaa taactctcat aacaggaagc cataatgacg gaacaagaaa
    12661 aaacctccgc cgtggttgaa gagactaggg aggccgtgga caccacgtca caacctgtcg
    12721 caacagaaaa aaagagtaag aacaataccg cattgattct cagcgcggcg gctatcgcta
    12781 ttgctctggc ggcgggcgtc ggtttgtatg gctggggtaa acaacaggcc gtcaatcaga
    12841 ccgccaccag cgatgccctg gctaaccaac tgacggcatt gcaaaaagcc caggagagcc
    12901 aaaaagccga actggaaggc attattaagc aacaggctac gcagcttgaa caggcgaatc
    12961 gtcagcaaga aacgctggca aaacagctgg atgaagtcca gcaaaaggtc gccaccattt
    13021 ccggcagcga tgctaaaacc tggctgctgg cccaggccga ttttctggtg aaactcgccg
    13081 gacggaagct gtggagcgat caggacgtca cgaccgctgc agcgttgctg aaaagtgcag
    13141 acgccagcct ggcggatatg aatgacccga gtctgattac cgttcgtcgg gcaattaccg
    13201 atgatatcgc cagcctttct gcagtatcgc aggtggatta tgacggcatc atccttaagc
    13261 ttaatcagct ttcaaatcag gtagataacc tgcgtctggc cgataatgac agcgatggtt
    13321 cgccgatgga ttcagacggt gaagagcttt ccagttccat cagcgaatgg cgtatcaatc
    13381 tgcaaaaaag ctggcagaac tttatggaca acttcattac gattcgccgt cgtgatgaca
    13441 ccgccgtacc gctgttagcg ccaaatcagg atatctatct gcgcgaaaat attcgctctc
    13501 gcctgctggt cgcagcacaa gctgtaccgc gtcatcagga agagacttat cgccaggcgc
    13561 tggagaacgt ctccacctgg gtacgtgctt actacgatac tgatgatgcc accaccaaag
    13621 cgttcctcga cgaggtggac cagttaagcc agcaaaatat ctcgatggat cttccggaaa
    13681 ccctgcaaag ccaggcgatg ctggaaaaac tgatgcagac ccgcgtgcgt aacctgctgg
    13741 cacaaccggc agcggggaca acggaagcta aacctgcacc tgcacctgca cctgcacctg
    13801 cacctgcacc tgcaccgcaa ggtgatactc cggcagccgc gccgcaagga gaataaccat
    13861 gctaaaagtg ttattactct ttgttttgct gattgcgggg atagtggttg gcccgatgat
    13921 tgccggtcat cagggttacg tgctgatcca gactgacaac tacaatatcg aaaccagcgt
    13981 cacgggcctg gcgattattt tgattctggc gatggtagtg ctgtttgcca ttgagtggct
    14041 actgcggcgg atcttccgca caggcgcaca cacccgtggg tggtttgtcg gacgtaagcg
    14101 tcgccgtgcc cgtaagcaga ccgaacaggc gctgctgaaa ctggcggaag gcgattatca
    14161 gcaagttgaa aagctgatgg cgaaaaatgc cgatcacgcg gaacaaccgg tggtgaacta
    14221 tctactggct gccgaagccg cgcaacaacg tggtgatgaa gcacgcgcca accaacatct
    14281 ggaacgcgca gcagagctgg ccggcaatga caccattccg gtagaaatca cccgtgtacg
    14341 tctgcaactg gcccgtaatg aaaaccatgc tgcacgccac ggcgtggata agctgctgga
    14401 agttacgcca cgccatccgg aagtattacg tctggcggaa caggcgtata tccgcacagg
    14461 tgcatggagt tctctgctgg atatcatccc atcaatggcg aaagcccatg ttggtgatga
    14521 agaacatcgt gcaatgctgg aacaacaggc atggattggc ctgatggatc aggcgcgtgc
    14581 cgataacggt agtgaaggtt tgcgtaactg gtggaaaaac caaagccgga aaacgcgtca
    14641 tcaggtagcg ttgcaggtgg caatggcgga acatcttatt gaatgtgacg atcatgatac
    14701 tgcccagcaa attatcatcg atggcctgaa acgccagtac gacgatcgcc tactgctgcc
    14761 gattcctcga ctgaaaacca acaatccgga acagctggaa aaagtgctgc gccagcagat
    14821 caaaaacgtc ggcgatcgcc cgctgttgtg gagcacactg ggccagtcac tgatgaagca
    14881 cggagaatgg caggaagcat cgctcgcctt ccgcgcagcg ctgaaacaac gtccggacgc
    14941 ctacgattac gcatggcttg ccgacgcgct ggacagactg cacaagccgg aagaagctgc
    15001 agcgatgcgt cgcgacggtt tgatgttaac gttgcagaac aacccaccac agtagttcct
    15061 tctcacccgg aggcaagcac ctccgggacc tttctgatgc ataaaaaaac gcctgctctt
    15121 attacggagc aggcgttaaa acaggtctgt atgacaacag tgggtgcttc actcaacgtt
    15181 gtgtccatgg tgtctgatga ggcataagcg acatctgtca gtggacgata agcaccgtaa
    15241 acggctctgc gtcattccgg agtttatgag gcactaaggc gaacataaga gatggaatga
    15301 gcatctaccc gtttattatg ccacagagaa tcgggaaata acatccctta acacttgtta
    15361 tgagataatt ctgtaatcct ctttgcttcc tgagtgaata tttaacctga gcttgatcct
    15421 acacatactt attatgaatg ataaaattca ttcaattaat aacacatata ttaattgccg
    15481 ttaaaactaa aaaagcatta ataatcaacg caatataata aacctgcctt acatatcaac
    15541 tgcgccagag gtaggattga aaacgctctc ctgattttcc aattcatttt ctggataaat
    15601 aaataattta tttttgtcac tattatttat gtaatcatcc tgtcagggag agggatctca
    15661 attatcaatg cttaattacg tcatcatttt gatgacatga aaccggagca ttttgcatgg
    15721 aattttcgtt ttcacccaaa cttcttgttg ttgctgtcgc cgccgctctt cctctcatgg
    15781 ccagcgcagc agataccccg tcaactgcca ccgcacgcaa aggctttgcc ggatacgatc
    15841 acccaaacca gtatccacag aacgctgcaa tttattgaat ttgcacgatc atgtaggccg
    15901 gataaggcgt ttacgccgca tccggcaatc aaccgcaggc ggccacaaaa tggccgcttt
    15961 tttacttatt caccaccagc aaatgtgcct gcataatgtc actggccggg cgaccgtgcg
    16021 ccagcaaatc agccattgct ttaagatatg gcggtagatg gcggaaataa cgctgatacc
    16081 cggcacacaa ataattcagc cccggtttgc cgctggcatc gagcatgaag cggtgtttcg
    16141 ggcagcctcc ccagcacgct tttaacacgt tacaactgcg acactgcgcc ggtaattgct
    16201 taaatttatc ttcaccaaac acctgctgtt gcggggaatc gatcatttct gcaattgttt
    16261 gctgatgcat attccccagc cgatattgcg gataaacata gtgatcgcag gcgtaaacgt
    16321 cgccgttgtg ctcaacaatc accgagcgcc cacaggttgg ctgatgatgg caaaccgcac
    16381 ccggcgcacc gacaaaattg gcaaacgccc attcgatatt catcacgaaa atcttgccga
    16441 cgtcgcgttt gatccagtgg tcgaatatcg ccaccagaaa ttcaccaaat tcatcggggc
    16501 gcaccgacca ttccgtcagt tccccctgaa tatcaccagg cgcatgcaat ttcagtccat
    16561 cgcgggcagc tgtttcatca gccagacgct cgaccaccgg aataaactgg atgaattcga
    16621 ctcccgcatc gcacaaaaaa tcataaacct gcaacggttg ctgcgcgctg gtgcggttga
    16681 cgcagaccag cacgttatag tcgacatgat gtttttgcag cagcgtcagg gcacgcatca
    16741 ccagcttatg cgttggtctg ccacctttgg tcacgcgata ttgattgtgg atctcagccg
    16801 gaccgtccag cgataaccca acaagaaaat gattttctgc cagaaatgca caccatttat
    16861 catcgagcag cacgccgtta gtctggaagc tgttacttat cttcctgcca gcaccgtatt
    16921 tcgcctgtaa ctttacggca cggcggtaaa aatccagccc gagtaacgtt ggttcgccgc
    16981 cctgccaggt aaaagccact tcgttttgcg gttcgctggc agcgatatag tgacggacat
    17041 acgcttccag cgtgtcatcg tccatatgcg tgactggctt ttcgcggtaa agggattgtt
    17101 tttcgagata aaaacagtag tcacagttca gattacaatc ggaaccactc ggtttcgcca
    17161 tcacatgaaa agcacgcgtt ggaacctgtt gcagcatggt cactccttgc ctgaagtcat
    17221 ccttcaatac gttaagggcg aaatcatacc gtggggcaat gagttattac gggcgtttat
    17281 tgcgaaggga gggtgacgaa atgcacagaa aacaaaaaac cccgccgaag cggggttcaa
    17341 aattggtcgg cgagagagga ttcgaacctc cgacccactg gtcccaaacc agttgcgcta
    17401 ccaagctgcg ctactcgccg aaatactgct ttttgaattt ttagttcaat tctttaaagt
    17461 cgtggtgcga ggggggggac ttgaaccccc acgtccgtaa ggacactaac acctgaagct
    17521 agcgcgtcta ccaattccgc caccttcgca ttgtcacaac ttctaataat ggggtggcta
    17581 atgggattcg aacccacgac aactggaatc acaatccagg gctctaccaa ctgagctata
    17641 gccaccacta caaatcttgt tacgcggtat tactaccacc gcagctcaag cgccgggact
    17701 aaatggcgcg cccgacagga ttcgaacctg agacctctgc ctccggaggg cagcgctcta
    17761 tccagctgag ctacgggcgc ttagcgccgt tgcgggggtg gataatacgg acttcccacc
    17821 cctctgtcta gtcccttttt aaataaattg cgcgtttggt tacggtttgt gcgttttgct
    17881 gcttattcct ccagtttatg cgctttcccg tggcgattaa ggccaaaaac tttataaatc
    17941 gccgtcaccg ccagcataaa gatgatgcca acaaacagcg acatacgcgt atcttcatta
    18001 aagtacatgc cgatcaaaac gcaaatcagg aatgccattg ttacgtagtt ggcccacggg
    18061 aacaggattg agcggaacgg atggctggca atcgccgctt tatgtgcacg ccgaaaacgc
    18121 agctggctta tcaatatcac aaaccatggc accatccccg gaagcacgct ggcactgtag
    18181 acgtagacaa acacacgctg cggattggga atgatgtagt tcaggcatga gccaattagc
    18241 agaatagcaa tagatactgc cacacccgca actggtacgc cgtgacggga aactttcgcc
    18301 atcgccgccg gtaactgacg gtttttcgcc agtgcgtaga gcatacgtcc gcaactgtac
    18361 atgccgctgt tacagccaga gagcgcagcc gtcagcacca caaagttgat aatgcccgcc
    18421 gctgcggtaa taccgatttt ggcaaaagtc agtacgaacg ggctgccgtt gctgccaatt
    18481 tcattccacg ggaagatggt gacgataacg aaaatcgcgc ctacgtagaa aatcaggatc
    18541 cgccacagca ctttgcctac agcactgcgc agcgtcacct gcggattctt cgcttcaccg
    18601 gcagtaatgc caatcagctc cacgccctgg taggacgcca ccacaataca cagcgcggtc
    18661 aggaaccctt tccagccacc cgcaaagaaa ccgccatgct ctgtgagatt gctaaaacca
    18721 atcgactgcc cgccattgcc aaagccaaag aaaatcacgc ccaggccaat gacaatcatc
    18781 acgataatcg tggtgacttt gatcatcgcg aaccagaact cgatttcgcc gtacaaccgc
    18841 accgccgcca gattcgccaa cgccaccagc gccaccgcga tcaatgcagg tatccactgc
    18901 gccatctccg ggaaccagaa ctggacataa acgccaatgg cggtgatttc tgagatcccc
    18961 accgccatcc acataaacca gtaagaccag gcggtgagat aaccaaaaaa cgggctcata
    19021 taacgatgcg cataaacggc gaacgaaccg gtaacaggtt caaggaacaa catttcgccc
    19081 attgaacgca tgatgaaaaa gacgaacagc ccggcgatga tataggccaa caatacggat
    19141 ggcccggccc atttcagggt actggcggcc cccataaaca ggccgacgcc aatagtgccc
    19201 cccagggcga tgagttcgat atgtcgagct tccagcccac gctgtagctc tggtttgtta
    19261 tctgccataa atcctcgtgt tgtgtttgca tgctttccgg tgttaccggt tatcgttatg
    19321 ggtacatcga gtgttgcgaa tgttttcgta attcaggaga aatggcaaat aaagcattaa
    19381 aaatttgaat gctttgtgta ataaaaaagc agacaggcga cggagtgacc actccgtcgc
    19441 tttacaaaga gaggaaaatc agaggttgcc ggtgtagtgc cagcgtaaat aacgcagcaa
    19501 acgaagctga cgcttaatgc ggctcggctg cgaaagcagg cggtagagcc actccagtcc
    19561 cagcgtttgc cagattttcg gtgcgcgttt tacgtgaccg gtgaaaacat cgtaagtccc
    19621 gccaacgccc atatacagcg catctggatg caccagacgg cagtcgcgca tgaagatctc
    19681 ctgctttggc gatcccatcg caacggtgac gatttgcgct ccgctggcat gaatgcgttc
    19741 aaacagcgcc tgacgctgct cgggtttaaa ataaccatcc tgactgccaa cgatattcac
    19801 attccattgg ttgcgcagtt tagcttcagt ttgcgccagc acttcaggtt taccgcccac
    19861 aagaaatacc ggcgtccctt ctttgcctgc gcgcgccatc agctcttccc agagatcggc
    19921 accggcaacg cgggaaacct gcgcctgcgg gtacttttta cgtactgaac gtacaacgct
    19981 gatgccatcc gcatatttaa attcggcagc gttaattaac tccctgacct cggcgttatc
    20041 ttcaatggtc agcatttttt cagcattaat ggcaaccagc gttccctgct taagctgccc
    20101 gtcagcaaac agataatcga gggcgtgctg catatcacgc caaccaatca actgtaagcc
    20161 acgcagcgta taggtcggtg ccgtggtgtt gttattcatt gttatccttc aacctgcgtc
    20221 cggagcgatg attttgtacg tttatgaatg agtccggcgc tttcaaaaag ccagtacagc
    20281 agttttgcga ttatcagaca tgcgccgaag accacgataa aaaagactac gcgtgagaca
    20341 aacgaatcca gcccttcacg cgccagcacg atcatattga agatcgcccc aaagcagaaa
    20401 ctgtgcaata tcgcagcctt atagcgatta ggctcgcggt tgcccagctc atacagccag
    20461 tcgaaccatt tgatgatcag accaaccacg atcgccccga gcgggatgaa caacgcgccg
    20521 cccatcacca ccagtgagcc tataagcgta ggcgagatcg ccagtccgga gtggttattc
    20581 agcacttccc aggtaaagta gttggctgag ttcagcacca tactcgggcg acccggccac
    20641 agccaggaag ggataaagac atagaaatcg cggacaattg gagccaggcc ctggaagtcg
    20701 atgttgtcgt agttctgcaa cagcaacgcc agattctccc acggggagaa ggtgtcgcga
    20761 gtgagataga gaaacgtata gaacgcttca tcgccgctca cattcattcc atagcgtttt
    20821 agcgccatcc agaacatgcc aacaatcccc agcacgcccg ccgccgccag catccacaac
    20881 gaaatccagc cgcgaataat gccaataaac aggaagatag cgaatgcgat gatgatattg
    20941 gcgcgagtgc cgccgacaat catataagtc agcaagccaa aggcgaccgt gctgacgagg
    21001 aaaaacagcc acgctttgct gtcctggcgc agaaagtaga ccaccagcat cgccgggatg
    21061 aaaaagtaaa agaagcgttt taacgccacg ccggagactt cactggagaa gatctgactg
    21121 taggagttaa gccggaacag caaaaagccg ttgtgcatga agaagatgcc gacgcttacc
    21181 agcgcgatac ccatcaggat cacccacgta agattggtct ccacgcggtt catggtaaac
    21241 agcggacggc gcggtacatc agcaacgcgt ttgcgtaggc gggttttgta ggtgacatag
    21301 taaaccgcgt agaagcagcc cgcagaaagc aacgcctgca acaagatttc tggcggcgcg
    21361 acaccaacat caaagcgaaa taccagcacg ctggtcagcg ggaagccgaa gaaaaaggtg
    21421 agcaaaaaca gcaatgaaaa gaagacattg aagttaaagc gcacacggcg aaactcaaac
    21481 caggtcagcg tggcaataaa cagcgtgcag agcagccaga ccacaaacag gccactgaat
    21541 tgcagcagac tcatgcgacc tccccggcgg caatcgccaa cgcccgctgc cagccttgta
    21601 gatagttagg gctaaagaag gcaatggtgt ttttatccac cgacgccaac tggcgctgcg
    21661 cttcacgcac aatatcctcg ttgagatcgt cggtagtaaa cagcaccggc aaatgctgtt
    21721 ccgtcatatc ctgccagaac ggattttccc ggttaagcac acaaggaatg cccgcctgaa
    21781 tcagtaagca aagcgtacca atgccctgct ggcgggcaaa aataaagtaa ccaagatcgc
    21841 actgacgaag tagcgccaga taggcgtcaa attccagttt ttcgctcaaa atttgcagat
    21901 tttcttcgct gaataactcc agccccgcct gacgaacttc ctcaatgtac gcttcgttat
    21961 taggcggata tcccatcggc accaccactt ttaccgtatc gccaaattgc tgatgaacgg
    22021 cgcgcaaggc agcaatatgg tcattgctgc ggtcaccgga gttccccacc agaatggtca
    22081 ttttcccttc acgttgccga tcattcgcca tcgtattgag agaagggtcc atccgcgtcg
    22141 ggaagtacag cagttcgccc cgcacctttg gatgcgtttt ggcaaaaaag ctcaaatcac
    22201 cgcgggtggc aaatacacag ccgactcgct tttgcgccag gcgacgtagc gggtaaaaaa
    22261 gcttatatct caagccactg gaaagctcat acaggtctgc cccccagata tgccagaaaa
    22321 actggctggg cttaatgcca ccactcagca gagccagcca cagtgtggga ttgaactgac
    22381 cgtggaaaaa aaatcgctgc tgacggttag cttttgcttt cgcgatgacc gcttccgcca
    22441 gcgatttttt cccagggaaa aattgcacag aaagcgccgg acagctatca cttaagccgt
    22501 cgtccttgcc aacaaccata aactcgcgcg cgtgctcgct cgtcgcggcc agcgcgtcat
    22561 tgaaaaaccg caaaacggtt cggttatggt gagggatatc cgatcccagt acgtgaatca
    22621 gtacagtcat gcccgcctac gccagagtaa aaacacgcca caacaaagag aaaaatagac
    22681 gatataagtt gccatatatg cctgcgccgc gcccagtgca ccatgcgcag ggattagcca
    22741 gtgggcaaat accatcaata aagtgaactg gctgacttcc gccagaatat aaaaccgcag
    22801 tgacgctttg gcgatcacca gataaccaaa gacataagcg cccactttta acacatcacc
    22861 cactaactgc caggcaaaga gatcgcgcat agcggtaaat ttattcgaca acagcaacca
    22921 gatagcaaaa tcacgcagca accagacggt aaaactcgcc gccgccactg ccggtaagac
    22981 gaatttcagc gatttaacca cttcccgggt gatatcgcgc ttttccgtta gccgcgacaa
    23041 cgtgggcagc aaatatacgc tgaacgatgc cgtaataaat tgcaggtagg catcggaaat
    23101 actgctcacc ccttgccaga tccccacttc atcccagcta tactgcgccg ccagcagttt
    23161 acgcatcatg atgtaagcaa caggcaaggt caccgacgta atcaacgcca tgagcgtaaa
    23221 tttgctcaac tgccctgcca gaccgttatc ccagctgggt ttcagatagc ttaacgggat
    23281 gacaccacgt ttgatcaaca tgatggcggc aggaatcacc accagcgcgg gaatcagcgc
    23341 cagacccagc aacgcccctt cataaccgcc caaacggtaa ctgacgtagt acgcgagaac
    23401 gccaatcaag ctgccgacaa tcagcgataa cgcattacct gcggcatcgc gaaagccttt
    23461 catcagcgcc agtaacaggt tgccccaggc gatccccatt tgcaccagcg ccactaaacg
    23521 caccagcccc tgatagtcgg tattaccaaa cagtccctgg ctgattggcg cagctgccag
    23581 cacaaaaacc agcgccatca gcgtagagaa accaagtacc atcgctgatg aagtgccgac
    23641 cacgcggcgc agctgttgcg gattatcatg gtactgggca acgtatttgg ttacaccgtt
    23701 aaagatgcca gctccggcaa gcacgccgag cacggtaatc aactggcgga aatttgccgc
    23761 cagcccaagc cccgccggac caaatgacac agccagcaac ttaccgacca gtaacccggc
    23821 accaatcttg accagtgtac tggccgccgt ccacaaggac gcttttgcca acgacatatc
    23881 aggagaagta gttcaacaaa gtcgcaatta ccgtacgctg attgacgggc gacaggttgt
    23941 agaacagcgg caggcgcagc aggcgctcgc tctctttggt ggtgtagcga tcttcaccgt
    24001 ggaactcacc aaagcgttcc cccgcagggc aaccgtgcag cggaatgtaa tggaacaccg
    24061 ccatgatttc cgcttctttc agaaagttaa tcaacgcgct ccggtcatca atatcccgca
    24121 gtttaatgta gaacatatgc gcgttctgca cgcagccatc gggaatcgac ggcagctcga
    24181 tacgcccggc tttcgccaga ggcgctaacg catcgtagta gttttgccac agcgccagac
    24241 gttgctggtt gatacgatcc gctgcttcca gttgcgccca caggtacgca gcttgcagat
    24301 cggacatcaa atagctggag ccaatatcgc gccaggtata tttatcgacc tgaccacgga
    24361 agaactggct gcggttagtg cccttttcac ggatgatctc ggctcgttcg attaacgctt
    24421 tatcgttaat cagcgtcgcg ccgccttcac cacccgccgt atagtttttg gtttcatgga
    24481 agctaaagca gccaatatga ccaatggttc ccagtgcacg ccctttgtaa gtggacatca
    24541 cgccctgagc ggcatcttct accacaaaca gattatgctt tttcgccaac gccataatgg
    24601 tgtccatttc gcaggccaca cccgcgtaat ggaccggcac gataacgcgc gttttgtcgg
    24661 tgatcgccgc ttcaatcagc gtttcgtcga tgttcatggt gtccgggcga acatccacaa
    24721 aaacgatttt tgcgccacgc agcacaaagg cattggcggt ggagacaaag gtgtagctcg
    24781 gcatgatcac ttcatcgcca ggctggatat cgagcagcag cgccgccatc tccagcgaag
    24841 cggtgcagga cggcgtcagt aacactttgg cgctgccaaa acgttgctcc agccactgct
    24901 ggcagcgacg ggtaaaaccg ccatcgccac acagtttgcc gctacccatt gccgactgca
    24961 tatagtcgag ttcggttccc accaccggcg gtgcgttaaa tggaatcatg tgatcacctg
    25021 tataaccagt acgcggtgct ttctacattc gcaccacttt gtatgtatcg tttaagcgcg
    25081 gcggtgttgc ccatttgggt cgccacccgc aaagttgttt taccgcgagc atacgcccag
    25141 tttagcgccg tttgcatcag ctcagcacct gcaccgcgtc cagccagcag gccaattcgc
    25201 gcatctgtcg cattgagttc ccgtaaagag acataaccgc gaataccgcc ggacgccgca
    25261 cgtaaaatca gacattgatg atcaaaggtg ccgcgcacgg cattttcaat ccactgtgca
    25321 taaaagcgtc cgctggcgtc aggcgcatac cacggcgcac gaaaacggct ttgcgcaaat
    25381 gcggcgctgg ctaactgacg taatgcggga atatcggtct cttgtgccac tacagcacca
    25441 ctatcactga cattgttcac gggtagtgcc aaatcaactt caccttctac cagggagaat
    25501 cccagctgtt gcagggcatc cagttcaccc gtatttgatg ccgcaatttt ggcctgcacc
    25561 cgtgaccacg gcgctaacac gtctggcgtc aggagcggtg cttcagacgt aatgcgcacg
    25621 atggcgctgt taacaccaaa gaaggcgttt tcccaggtta gtggctcaat actggcgcgg
    25681 acgggcacga agtaactcca gcagatattg gccgtagcca gttttcgcta atgaactggc
    25741 agcacgcttc acaccctcgt catcgagcca gccgttacgc caggcgatct cttccaggca
    25801 ggcaatctta aagccctggc gtttttctac tgtctgcaca aaggtactgg cttcaatcag
    25861 gctgtcgtga gtgccagtgt ccagccaggc aaatccgcgc ccgagcagtt caacggtcag
    25921 gttgcccgcc tcgaggtaca tctggttgat ggaggtaatc tccagttcac cacgctccga
    25981 cggcttcacc tgctttgcgt actccacgac tttactgtcg tagaaataaa gcccggtcac
    26041 cgcccagttt gacttcggct gttttggctt ttcttccagc gagatagcgc ggaaattgtc
    26101 gtcaaactcc accacgccaa agcgttccgg gtccatcacc tgatagccaa ataccgtcgc
    26161 cccttccgtg cgcgccgcaa catgacgcag cttcggactg aagccctgac cgaagaagat
    26221 gttatcgccc agcaccagac aagaaggttc accgttgagg aaggtttcac cgatgataaa
    26281 ggcctgcgcc agaccgtccg ggctgggctg ttcggcgtat tccagctgga taccgaactc
    26341 actaccatcg cccagcaggc gctggaaata acctttatct tccggcgtag tgatgatgag
    26401 aatttcgcgg atatcggcca acatcagcac cgacagcgga tagtaaatca ttggcttatc
    26461 gtaaatcggc aacagttgct tcgatacgcc gcgcgtaatc ggatgcaatc gggtgccgga
    26521 accgcccgcc aggataatac ctttcatgtt tgcctccgct gaaaattagc ctttcagacc
    26581 taaacgctcg ccctgatagc tgccgtcctg cacctgcttc caccagcttt cattagccag
    26641 ataccactgc accgttttac gcattccact ttcaaaggtt tcctgtggca gccagccaag
    26701 ttcacgggca attttcgaag catcaatggc ataacgcaga tcatgccccg gacggtcagc
    26761 gacaaaggtg atcaagtcac gatagtgcac cacgccgtgc ggcttgttcg gagccagttc
    26821 ttccagcagc tcgcaaatgg tttccacaac atcgagattc ttacgctcgt tgtgaccacc
    26881 aatattatag gtttcaccga ctttcccggt ggtcgccacg caatacagcg cgcgggcgtg
    26941 atcttccaca tacagccagt cacggatttg ctgcccgttg ccatataccg gcagcggttt
    27001 acccgccagc gcgttgagga tcatcagcgg gatcagtttt tccggaaagt ggtaaggacc
    27061 gtagttattc gagcagttgg tgataagcgt cggcagaccg taggtccgca gccaggcgcg
    27121 caccagatgg tcgctgctgg cttttgacgc ggaataaggg ctgctcggcg catacggcgt
    27181 ggtttcggtg aagaaatcat ccgtcgagtg caggtcacca tatacttcgt cggtggagat
    27241 atgatgaaaa cggaacgctg attttttatc ttccgtcagc gtattccagt aagcccgcgc
    27301 cgcttcaagc aatgtataag tcccgacaat gttggtttca ataaacgctg ccgggccgtc
    27361 aatagaacgg tcaacatggc tttcggctgc cagatgcatg acacagtctg gctgatgctc
    27421 agtgaatacg cgtgccagtt ctgcccgatc gcagatatca actttctcaa aggcaaagcg
    27481 ctcgctttgc gcgaccggtg ccagcgacat caggtttccg gcgtaggtca gcttatcgac
    27541 cactaccacc gcgtcgctcg tttcgttgat gatataacgc accagcgccg agccaataaa
    27601 cccggcacca cctgttatca gaatttttct catcagcgcc agactccttt ggcatcgacg
    27661 acatactgct gatggacatt gtcgccattg ataactttaa actgactatg atcgaccagc
    27721 atcaccagca cgtctgccgt tgccagcgcc tcgtcaagct gcgccagagt acaaagcccg
    27781 gtcagttttt tcggcaactg gtggatgtta ggctcaacaa ccagagtttc gccgctatgc
    27841 cactgggcga tcagttcagc gatttccatc gccgggcttt cgcgcaggtc atcaatattc
    27901 ggtttaaacg ccagaccaaa gcaggcgatt ttcagttcac tggcgcgttt atcggtagcc
    27961 gccaggcaat cagccaccgc cgctttcacc tgatcgataa cccagaacgg tttgtgatcg
    28021 ttcacttcgc gcgcggtacg gataagccgc gcctgctggg ggttctgtgc cacgataaac
    28081 cacggatcaa cagcaatgca gtgaccgccc acgccagggc caggctgaag aatattaacg
    28141 cgagggtgac gattcgccag gcgaatcagt tcccagacgt taatcccctg atcggcacaa
    28201 atcagcgaca attcattagc aaaagcgata ttcacatcgc ggaagctgtt ttcggtgagc
    28261 ttacacattt ccgccgtccg cgagttagtg acgacacact caccttcgag gaaaattttg
    28321 tacagttcgc tggcgcgggt cgaacaaacc ggcgtcatac caccaatcac gcgatcgttt
    28381 ttaatcagct cgaccataac ctggcccggt aacacgcgtt ccgggcagta agcaatgttg
    28441 acgtccgcct gctcgcccac ctgctgtggg aaagtgagat ccggacgcat ctctgctaac
    28501 cattctgcca tcttctcggt tgaccccacc ggcgaggtgg attcaaggat caccagcgcg
    28561 ccttttttca gcactggcgc aatggagcga gcagccgatt caacgtaggt catatctggc
    28621 tcatgatcgc ctttaaacgg cgtgggtaca gcaatcagcc aggcatccgc ttcaactggc
    28681 gtcgtgctcg ctcgtaaaaa accgccttct acggcagttt ttactacgct cgccaaatca
    28741 ggctcgacga tatggatttc gccacgattg atggtatcaa ccgcgtgttg gttgatatcg
    28801 acaccaatca cctgtttttg ccgtgaggca aacgctgctg ctgtaggcag cccgatgtaa
    28861 cccagtccga taacagaaat ggtcgcaaaa ctcatagtga tatccgatta ttttttaacg
    28921 cttccagaat gcgagagcat gcctgaccat caccatacgg gttatgggcg cggctcatag
    28981 tttgatattc gttttcgtct tttaaaagac gcgtcacttc ctcgacaatt cgctgcttat
    29041 ccgtgcctac cagacgcacc gtacccgccg tcaccgcttc cggacgctca gtggtatcgc
    29101 gcatcaccag caccggtttc cccagcgaag gcgcttcttc ctgaatgccg cctgagtcgg
    29161 tcaaaatcag ccaggcgtgg ttcatcagcc agacaaacgg taaatactcc tggggatcga
    29221 tcagaatgac atttttcaca tgccccagaa tgcgattgac cggctctctg acgttcgggt
    29281 tgagatgcac cggatagaca atctggatgt cctggtgcgt ggtggcaatg tctgccaacg
    29341 cgtggcagat ttcttcaaag ccacgaccga aactttcacg cctgtgaccg gtcaccagaa
    29401 tcatcttttt atcggggtcg ataaacgggt aatttgccgc cagttctgaa cgcagcgtat
    29461 cgctgctcat cacctggtca cgcacccata acagtgcatc aataactgta ttaccagtaa
    29521 tgaagattcg gctatccgca acgttttcac gcagcaagtt ttgccgggaa gtttcggttg
    29581 gagagaagtg atacatcgcc agatgcccgg tcaatgtacg gttagcctct tccggccacg
    29641 gcgaataaag atcgcccgtg cgcagaccag cctcaacgtg accaacagga atacgctgat
    29701 aaaacgccgc caggctggtt gccagcgtcg tcgttgtgtc gccgtgaacc agcacgacgt
    29761 ctggtttgaa ctcggcaaga ataggtttta gcccttccag aatccgacag gttatctctg
    29821 tcaggccctg tcctggctgc attatgttga gatcgtagtc aggtacaatg gaaaagagtt
    29881 tcagcacctg atcgagcatc tcccgatgct gcgcagtgac gcaaacttta gcctcaaaaa
    29941 aaggatcttt tgccaacgca tgcaccaacg gcgccatctt gatggcttcc gggcgcgtac
    30001 caaatacagt cagtactttc acatcgattc tcttcgaata agcggcgagc gcctttgcgc
    30061 tcaccgcagc agtgttgcta tttcgagcaa cggcgggtta atgcgacacc agccccgatc
    30121 agccccccga caatgcccca cataatcatc aggaaggcac gacgtgggct atcgcgtttt
    30181 accggttctt ccggcgtacg caaatagcga taggtctgaa aacgcggatc cagggttgga
    30241 ccaacattca gggtgtttaa catggcccga ttctgatcat agtcgagatc aaaggccgga
    30301 ccgacggcct gtaaattttc cagtcgagcc tggagcattg gacgcccaag caggaacatt
    30361 tctgaatcag gtaattcctc ggcaggcaca tctgtcgcac tgcgcgaaat attatgctgc
    30421 tcagcaattt tcagcgcctg ttcaatactg ttcatccggc ggtcgtagat ggctttcgcc
    30481 acctcttcct gacgcttcac ctgtgctttc atctggatgg tacgcgccgc ccatgcgcct
    30541 ttcagctcat cattcagatg gctggctgca cgctggctgg caaaagcaac atactgacgt
    30601 aacaggttat tagcgtcagg cgcggtttca gcaataagct tcacgctgtc attgaccgcg
    30661 cgggtaaagt ctccggggat aaactggatg ttgttaatca tttcatccag caacgccgca
    30721 tcggctttgc tgttgcccac catccgctgt ttgtaatagt cggtttgcag ccagaactct
    30781 ctgcgggtat cccacgaggc cagttgcata acaaactctt tataggcttc gtccatgacc
    30841 gatggttggt cggcagaagc catgtttgaa cggacatcca ggttacgcaa aaattgctgc
    30901 tgcgagtaat atccccccag catattcacc gttggacgat cggtaatcgc cgtcgagctc
    30961 cactcctgac gagcaaaaaa agtatacgcc agcgcgatta acgcaaatgc cagccccatg
    31021 ccaataatcc atagcttccc agcccacaag gtacgaaaca acccacgaat atccagttca
    31081 ttttcagcgt cttcggccgg tttcccaggc attggttgtg tcatcacatc ctcatttatt
    31141 tggttaaatt ggggctgcca ccacgatttc tacgcagtct gcgttttacg cgcttaataa
    31201 agcgagcaac tttccaggca cgcttaatgc aatatccata gaggaagaat gctagcaaaa
    31261 agagcaccag catgacccac tccgggacaa aatgagaata ttctgccagc acgccaatgg
    31321 aagcgagcag tgccgcagca agggtaatca gcacaaacgc ctgacgggaa gtaaacccgg
    31381 cacgcatgat caaatggtga atatgctgac ggtcaggaga gaatgggctc atgcctttac
    31441 gcaggcgacg gtacataatc gccaccatat ccattagcgg aatggcgatt atccacaaag
    31501 cggtaaccgg gctgatggga tgggttttgc cctgggtcgt ttcgagcagg atccagataa
    31561 cggtaaaacc aatcagcgta ctgcccgcat cacccataaa gactttgtag cggcgaccca
    31621 ggataccaag gttaagcatg atgtatggca ggatggcggc gatcatcgca aagcaccaga
    31681 ttgcgaggct ggtttgcccg tcgaaccaca aaatcatacc gattgctgca aacgagacgc
    31741 aggacaaccc gcccagcaag ccatcaatgc catcaaccat gttgaacgca ttaatggccg
    31801 cccagacggc aaatagcgtc aggaagtaac caaacggtcc gagcaccatc tcccaggagc
    31861 caaagatata acccaggcta ctgagataaa gtttgccgaa caccatcata acaatgccaa
    31921 cagcggcctg tatggtggca cggattttta cgctgatatc aaaacggtca tccagcgcgc
    31981 caatgaaaac aagcacaccg gcacaagcga gatagagaga tgcatgcgga atatagtaat
    32041 cgacaattcc gaacgtgaag caaatccctg cgtaaaccga aatcccccca acgagaggta
    32101 tcaatccctg gtgacgtttg cggaagtttg gtttatccac taaaccgact ttttttgcca
    32161 ccttacgggc aaaaaacaga aacagtgtcg tgaataaaaa aatactgatg agatcagtac
    32221 tcactgtcag taaattcaca atgcatgctc tcagagaaaa ttattagcag aagtataacc
    32281 acgaagacct ttattcagaa gggaaagtcc gttccgaacc caccagtcct gtgcaaaaat
    32341 taaccatttg atatgcaaat tgcataacac gccagcaaat tccaggaatt ttcctaacat
    32401 cgccagcgcg gcattaagat tacagatata aaagcaaaac gccacgtaaa cacgtggcgt
    32461 ttttggcata agacaaattt atgagcgttt catcatttcg aagaaatcgt cattggtctt
    32521 ggtcatggcc agtttattaa tgaggaattc cattgcatcg atttcgccca tcgggtgaat
    32581 gattttgcgc aggatccaca ttttctgcag ttcttcctga gtagtgagca gttcttcttt
    32641 acgggtaccg gaacggttgt agtcgatagc cgggaagacg cgtttttcag cgatcttacg
    32701 agagaggtgc agttccatgt tgcctgtacc tttaaactct tcgtagataa cttcgtccat
    32761 tttagaaccg gtatcgataa gcgccgtcgc gataatagtc aggctgccgc cctcttccac
    32821 gttacgcgcc gcaccaaaga agcgtttcgg acgatgcagg gcgttggcat ccacaccacc
    32881 ggtcaacact ttacctgacg ccggaacaac ggtgttgtaa gcgcgcgcca gacgagtgat
    32941 ggagtcgagc agaatgataa cgtctttctt gtgctcaacc aggcgtttgg ccttctcgat
    33001 caccatttcc gcaacctgaa cgtggcgaga tgcgggttcg tcaaaggtag aagcaacaac
    33061 ttcacctttt accagacgct gcatctcggt tacttcttcc ggacgttcgt cgatcagcag
    33121 aaccatcagc acacaatccg ggtggttgta agcaatgctc tgggcaatgt tctgcagcag
    33181 catggtttta ccggctttcg gcggtgccac aatcagacca cgctgaccac gaccgatagg
    33241 tgatgccaga tccagtacgc gagcagttaa atcttcagta gaaccgttac cacgttccat
    33301 acgcagacga gaatttgcgt gcagcggggt taagttctca aagaggattt tgttgcgggc
    33361 gttttcaggt ttgtcgaagt taacttcgtt aactttcagc agcgcaaaat agcgttcacc
    33421 ttctttcggc gggcgaatct taccagagat ggtatcacca gtgcggaggt tgaaacggcg
    33481 gatttggcta ggggaaacgt agatgtcatc aggaccggcg aggtaggagc tgtctgcgga
    33541 acggaggaaa ccaaatccat cctgcaatat ctccagtacg ccatcaccaa agatatcttc
    33601 gccactcttt gcgtgctgct tcaggatggc aaaaataatg tcctgcttac gcatgcgagc
    33661 caggttttcc agccccatat tttcgccgag agtgatcagc tcagaaaccg gcgtattctt
    33721 taattcggta agattcatag tggtgtgagt tcttaaactt ggggtaaatc tcgaacttaa
    33781 tgttgtgaat ggtatggcag gatcatccat gcctgtttac ggtcatcgac tcatgtcttt
    33841 tcgctgcctg gtcacaggag agtacgcaga actgaaacga caagacggat tgagtgacga
    33901 gccagaaatc tgttcacttc gcatttaaga taaaacggga agcgttggaa attacaagat
    33961 tcaaatctta ataaggtatg tttaatacga agtcaacact aagttagcat gactcacgcc
    34021 gggcgtccag tttttagcaa cggggcaccc gaacatgaaa ttcccttacg ccaggttagc
    34081 gtcgaggaac tctttcaact gacctttaga cagtgcgccc actttggttg ccgccacttc
    34141 accgtttttg aacagcagca gagtcgggat accacggatg ccatatttcg gcgcagtgcc
    34201 agggttttga tcgatgttca gttttgcaac ggtcagtttg ccctgatatt cgtcagcgat
    34261 ttcatccaga atcggggcga tcattttgca cggaccgcac cactctgccc agaaatcgac
    34321 gaggatcgcc ccgtccgctt tgagtacatc cgtgtcaaaa ctgtcgtcag tcaggtgaat
    34381 aattttatcg ctcatatata actccacagg aataagcctg gcgtgttggt ttcgttgttg
    34441 gtgtaacatt aaccaactaa aggttgactt tatttcaccg gatacgcttt cgtaaagcaa
    34501 tagtaagctg atattctacc acactatgag caaaacacat ttaacagaac agaagttttc
    34561 cgacttcgcc ctgcatccga aggttgtaga agcccttgaa aaaaaagggt ttcataactg
    34621 tacgcccatt caggcactgg cccttccgct gacgctggcg ggtcgtgacg tagccgggca
    34681 ggcgcaaacc ggtaccggga aaacgatggc gtttcttacg tcaacgtttc attatcttct
    34741 ctctcatcct gcgattgccg atcgcaaggt gaatcaaccg cgtgccttaa ttatggcacc
    34801 gacgcgtgaa cttgccgtgc agatccatgc cgacgcagaa ccgctggcgg aagctactgg
    34861 cctgaagctg ggtctggctt acggtggtga tggctacgac aaacagctga aagtgctgga
    34921 aagcggcgtt gacattctga ttggcaccac gggtcgttta attgactacg ccaagcagaa
    34981 ccacattaac ctcggtgcca ttcaggtagt ggttctggat gaagccgatc gcatgtacga
    35041 tctgggcttt attaaagata tccgctggct gttccgccgt atgccgcctg caaaccagcg
    35101 cctcaacatg ctgttctccg ccacgctttc gtaccgggta cgtgaactgg cgttcgagca
    35161 gatgaacaat gccgaatata ttgaagtgga accggaacag aaaacgggcc accgtattaa
    35221 agaagagctt ttctaccctt ctaacgaaga aaaaatgcgt ttgctgcaaa cgctgatcga
    35281 agaagagtgg ccagacagag cgattatttt cgccaatacc aaacaccgtt gtgaagagat
    35341 ctggggccac ctggcggcag atggtcatcg tgtcggtttg ttgacaggcg atgtcgcgca
    35401 gaaaaaacgt ctgcgtattc ttgatgaatt tacccgtggc gatctggata ttctggttgc
    35461 caccgacgtt gccgcgcgtg gtttgcatat tccggcagtg acgcacgtct ttaactacga
    35521 tttacccgat gactgtgaag attacgttca ccgtattggt cgtacaggtc gcgcaggcgc
    35581 aagcggtcac tctatcagcc tggcgtgtga agagtatgca ttgaatttgc ctgctattga
    35641 aacctatatt ggtcactcaa ttccggtaag caaatacaat ccggacgcat tgatgaccga
    35701 tctgccaaaa ccgctgcgcc tcacgcgccc acgtacaggc aatggtccgc gtcgtactgg
    35761 cgctccgcgt aatcgtcgtc gttcaggtta aataaaatgt catactcaaa atcattcaaa
    35821 ctgtatcaag atggcaagag aatgaatccc gatgagcata ctgtagtaag tgattcaggt
    35881 gaaagaatgc agccaacaca gagacagatt gaaggatgaa gagtatgggt tccacctcgt
    35941 cgctgtatgc agccattgat ctcggttcga atagttttca tatgctggtt gtgcgcgagg
    36001 tggctggaag catccagacg ctgacgcgaa taaaacgcaa agtgcgtctg gctgctggcc
    36061 tgaatagcga aaatgccctg tctaatgaag caatggagcg cggttggcaa tgtctgcgcc
    36121 tgtttgctga acgtctgcaa gatatccctc cctcgcaaat tcgcgttgtc gctacggcga
    36181 cattacgtct tgccgtcaat gcgggtgatt ttattgccaa tgcgcaggaa atcctcggtt
    36241 gtccggtaca ggtgatcagc ggtgaagagg aagcacgtct gatttatcag ggcgttgctc
    36301 acacaactgg cggtgccgat cagcgcctgg tggtggatat aggcggtgcc agtactgaac
    36361 tggtaaccgg cacgggtgca caaaccacct cgttgttcag cctgtcgatg ggctgcgtca
    36421 cctggctgga acgctatttt gccgatcgta atctggggca ggaaaatttt gatgctgcag
    36481 aaaaagcggc acgcgaagtg ttacgtccgg ttgccgatga attacggtat cacggctgga
    36541 aagtgtgcgt tggtgcttcc ggcaccgtgc aggcgttaca ggaaatcatg atggcacagg
    36601 ggatggatga acgcattacc ctggaaaagt tgcagcaatt gaaacagcga gccattcatt
    36661 gcggtcggct ggaggaactg gagattgacg ggctgacgct ggaacgtgcg ttagtgttcc
    36721 cgagtggtct ggcgatcctg atcgccattt ttaccgaact gaatattcag tgtatgaccc
    36781 tggcgggcgg tgcgctgcgt gaaggcctgg tctacggcat gttgcatctg gccgtcgagc
    36841 aggatattcg cagccgtacg ctgcgtaata ttcagcgccg ctttatgatc gatattgatc
    36901 aggcgcagcg cgtagccaaa gttgcggcta acttcttcga tcaggtggaa aaggaatggc
    36961 atcttgaagc aataagccgc gatttgctca tcagcgcctg tcagcttcat gaaatcggcc
    37021 tgagcgttga ctttaaacaa gcgccgcaac acgctgctta tctggtgcgt aatctggatc
    37081 ttcccggttt tacccccgca cagaaaaaat tgctggcgac gctactgctc aaccagacta
    37141 atccggtcga tctctcatcg ctgcatcagc aaaatgccgt accgccgcgc gtcgcagaac
    37201 aactctgccg tttactccgc ctggccatca tttttgccag ccgtcgccgt gacgatctcg
    37261 tgccagagat gacattacag gctaaccatg aactgttgac cttgacgctt ccgcaaggtt
    37321 ggctaaccca acatccgctg ggtaaagaga ttattgctca ggaaagccag tggcagagct
    37381 atgtccactg gccgctggaa gtgcattaat gagtaagtgc cggatgcgat gctgacgcat
    37441 cttttccggc cttgattatt tccctcgttt tgccgccatc atcgctttca gattcgccag
    37501 atggctttgc cctttctgca tccgttcttc ggcgctgacc actttgcgct cctgttccca
    37561 aatcagatca tcctgcggca gctccagcaa aaagcggctc ggctccgggc gcaccagctc
    37621 gccgtactga cggcgttctt tacacagcgt aaaggtcaat tccttctggg cgcgggtaat
    37681 gccgacatag gccagccgcc gctcctcatc gatattatct tcatcgatgc tgctctggtg
    37741 cggcaaaaac ccttcttcca taccgaccat gtagacataa ggaaactcca gcccttttga
    37801 cgcgtgcaga gtcatcagtt gcacctgatc cagctcttct tcactctcac cacgctccat
    37861 catgtcgcgc aaagtaaagc gcgtcaccac ctgggtgagc gtcatcggct catccagttc
    37921 gctgccttcc agcatctccg tcatccagct aaacagttgg ttgacgttct tcatgcgcat
    37981 ttcggcggct ttcgggctgg gcgatgtttc gtacagccag gattcataat ccatgccgtg
    38041 gatcagatcg cgcaccgcgg caatcggctc ccgctccgcc agacgctgga tttctgccaa
    38101 ccagtgagta aagcgggtca atgcttcata accacgtccg ctaagcgtct gactcaggcc
    38161 catatcaaag ctggcggtaa acatgctttt attgcgcgtc atcgcccact cacccagctt
    38221 tttcagcgtc gccgggccaa tctctctctt cggcgtgtta acgatacgca gaaatgcgct
    38281 gtcatcgtcc gggttagtca gcacgcgcag ataagccagc aagtccttga tttcaggacg
    38341 agagaaaaac gacgtaccac cagaaatttt gtacgggatg cggttttgca tcaggaattt
    38401 ttcaaacact cgcgactgat ggttaccgcg ataaagaata gcgtaatctt tgtactgcgt
    38461 tttattgacg aagtgatgcg caatcagttc accagtgacg cgctcggctt catgttcttc
    38521 gttattcgcg cttaaaactt taagctctgt gccgtaaccc agttcggaga acagacgctt
    38581 ctcaaagacg tgcgggttat tggcgatcag gatgttcgcc gctttcaaaa tacgtccgga
    38641 agagcggtag ttctgctcaa gcttaatcac cttcagcgcc ggaaaatcct gactcagcag
    38701 caccaggttt tgtggacgtg cgccgcgcca ggagtagatc gactggtcgt catcgcccac
    38761 cacggtaaag cgcgcgcggc tgcccaccag cagtttcacc agttcatact ggctggtgtt
    38821 ggtgtcctga tactcatcca ccagcagata gcgaattttg ttctgccagc gctcgcggac
    38881 ttcttcattg cgttgcagca gcaacgtcgg caataaaatc agatcatcga agtcgagaac
    38941 attgcacgct ttcaggtgtg catcatacag cccataacaa tgggcaaaaa tacggtcccg
    39001 ctcgccaatc gcactggctg ccgcctggga cggtgttttg agatcgttct tccagttaga
    39061 gatggtcgaa atcaactgtt gcaggagaac tttgtcatct tcaatcagcc cttcggtcaa
    39121 ctctttgagc aacgccagct gatcggtatc gtcaaacagc gaaaagttcg ccttcatccc
    39181 cagcgccgca tactcacgct tgatgatatc cagccccagc gtgtggaagg ttgagatcat
    39241 cagcccacgc gcctctttgc gacccagcgt ctgtccgaca cgctctttca tctcgcgcgc
    39301 cgccttattg gtaaaggtca ccgccgcgat atgtcgcgcc tgataaccgc aaccgcggat
    39361 cagatgggcg attttattgg tgatcacacg agttttaccg gaacccgcgc ccgccagcac
    39421 caggcagggg ccggtaacga attcgacagc ttgttgttgg ccggggttta gacgcatagg
    39481 tgtattgctc aatcttcgaa cggggggagg attgtagcag aatcgcattt gaatgtgacc
    39541 gcgtgtaagc cgctaaccga ttggctgcgg atgtttaaat cagagaggga agaccgaaac
    39601 cggagttcat cgtgaatcca ttagacttag aaaatatcgg gtcgtaacgt tgtgttatta
    39661 ctttttcaga gtttactaag gagaggtgta ctcgtggcga ttaatttcag tcctaaggtt
    39721 ggtgaaatac tggattgcaa ctttggtaac tatcctgtta gccagaatgg acagttcagc
    39781 accacgtatt acgatggccg cattcctcct gaaatgataa aaaacaggtt agt
//
LOCUS       DAESHI010000030        39066 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.030, whole genome shotgun sequence.
ACCESSION   DAESHI010000030 DAESHI010000000
VERSION     DAESHI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39066
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.030"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..906)
                     /locus_tag="KW011_001584"
     CDS             complement(<1..906)
                     /locus_tag="KW011_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HBH7659943.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPPGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDI"
     gene            1602..3887
                     /gene="nrdA"
                     /locus_tag="KW011_001585"
     CDS             1602..3887
                     /gene="nrdA"
                     /locus_tag="KW011_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HBH7659944.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            4121..5251
                     /gene="nrdB"
                     /locus_tag="KW011_001586"
     CDS             4121..5251
                     /gene="nrdB"
                     /locus_tag="KW011_001586"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HBH7659945.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            5251..5505
                     /gene="yfaE"
                     /locus_tag="KW011_001587"
     CDS             5251..5505
                     /gene="yfaE"
                     /locus_tag="KW011_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HBH7659946.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(5582..6208)
                     /gene="inaA"
                     /locus_tag="KW011_001588"
     CDS             complement(5582..6208)
                     /gene="inaA"
                     /locus_tag="KW011_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HBH7659947.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRNKAINHDFRQLENIWSQSPKRTGSR"
     gene            6387..6629
                     /locus_tag="KW011_001589"
                     /pseudo
     CDS             6387..6629
                     /locus_tag="KW011_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(6671..7747)
                     /gene="glpQ"
                     /locus_tag="KW011_001590"
     CDS             complement(6671..7747)
                     /gene="glpQ"
                     /locus_tag="KW011_001590"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBH7659948.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(7752..9110)
                     /gene="glpT"
                     /locus_tag="KW011_001591"
     CDS             complement(7752..9110)
                     /gene="glpT"
                     /locus_tag="KW011_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HBH7659949.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            9383..11011
                     /gene="glpA"
                     /locus_tag="KW011_001592"
     CDS             9383..11011
                     /gene="glpA"
                     /locus_tag="KW011_001592"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HBH7659950.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            11001..12260
                     /gene="glpB"
                     /locus_tag="KW011_001593"
     CDS             11001..12260
                     /gene="glpB"
                     /locus_tag="KW011_001593"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HBH7659951.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            12257..13447
                     /gene="glpC"
                     /locus_tag="KW011_001594"
     CDS             12257..13447
                     /gene="glpC"
                     /locus_tag="KW011_001594"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HBH7659952.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            13640..14542
                     /locus_tag="KW011_001595"
     CDS             13640..14542
                     /locus_tag="KW011_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HBH7659953.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(14583..15386)
                     /gene="yfaU"
                     /locus_tag="KW011_001596"
     CDS             complement(14583..15386)
                     /gene="yfaU"
                     /locus_tag="KW011_001596"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HBH7659954.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(15404..16693)
                     /locus_tag="KW011_001597"
     CDS             complement(15404..16693)
                     /locus_tag="KW011_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH7659955.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(16750..17955)
                     /gene="rhmD"
                     /locus_tag="KW011_001598"
     CDS             complement(16750..17955)
                     /gene="rhmD"
                     /locus_tag="KW011_001598"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HBH7659956.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAVMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            complement(17970..18752)
                     /locus_tag="KW011_001599"
     CDS             complement(17970..18752)
                     /locus_tag="KW011_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBH7659957.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(18972..20174)
                     /locus_tag="KW011_001600"
     CDS             complement(18972..20174)
                     /locus_tag="KW011_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HBH7659958.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(20274..20816)
                     /locus_tag="KW011_001601"
     CDS             complement(20274..20816)
                     /locus_tag="KW011_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659959.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            21095..21520
                     /gene="nudI"
                     /locus_tag="KW011_001602"
     CDS             21095..21520
                     /gene="nudI"
                     /locus_tag="KW011_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HBH7659960.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(21559..22161)
                     /locus_tag="KW011_001603"
     CDS             complement(21559..22161)
                     /locus_tag="KW011_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBH7659961.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            22469..23608
                     /gene="arnB"
                     /locus_tag="KW011_001604"
     CDS             22469..23608
                     /gene="arnB"
                     /locus_tag="KW011_001604"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HBH7659962.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            23612..24580
                     /gene="arnC"
                     /locus_tag="KW011_001605"
     CDS             23612..24580
                     /gene="arnC"
                     /locus_tag="KW011_001605"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HBH7659963.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            24580..26562
                     /gene="arnA"
                     /locus_tag="KW011_001606"
     CDS             24580..26562
                     /gene="arnA"
                     /locus_tag="KW011_001606"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HBH7659964.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            26559..27449
                     /locus_tag="KW011_001607"
     CDS             26559..27449
                     /locus_tag="KW011_001607"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HBH7659965.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            27449..29101
                     /gene="arnT"
                     /locus_tag="KW011_001608"
     CDS             27449..29101
                     /gene="arnT"
                     /locus_tag="KW011_001608"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HBH7659966.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            29098..29433
                     /gene="arnE"
                     /locus_tag="KW011_001609"
     CDS             29098..29433
                     /gene="arnE"
                     /locus_tag="KW011_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HBH7659967.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            29433..29819
                     /gene="arnF"
                     /locus_tag="KW011_001610"
     CDS             29433..29819
                     /gene="arnF"
                     /locus_tag="KW011_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HBH7659968.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(29813..30079)
                     /gene="pmrD"
                     /locus_tag="KW011_001611"
     CDS             complement(29813..30079)
                     /gene="pmrD"
                     /locus_tag="KW011_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HBH7659969.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(30189..31544)
                     /gene="menE"
                     /locus_tag="KW011_001612"
     CDS             complement(30189..31544)
                     /gene="menE"
                     /locus_tag="KW011_001612"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HBH7659970.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     D"
     gene            complement(31541..32503)
                     /gene="menC"
                     /locus_tag="KW011_001613"
     CDS             complement(31541..32503)
                     /gene="menC"
                     /locus_tag="KW011_001613"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HBH7659971.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(32503..33360)
                     /gene="menB"
                     /locus_tag="KW011_001614"
     CDS             complement(32503..33360)
                     /gene="menB"
                     /locus_tag="KW011_001614"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HBH7659972.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(33375..34133)
                     /gene="menH"
                     /locus_tag="KW011_001615"
     CDS             complement(33375..34133)
                     /gene="menH"
                     /locus_tag="KW011_001615"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HBH7659973.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(34130..35800)
                     /gene="menD"
                     /locus_tag="KW011_001616"
     CDS             complement(34130..35800)
                     /gene="menD"
                     /locus_tag="KW011_001616"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HBH7659974.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(35889..37184)
                     /gene="menF"
                     /locus_tag="KW011_001617"
     CDS             complement(35889..37184)
                     /gene="menF"
                     /locus_tag="KW011_001617"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HBH7659975.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(37263..37568)
                     /gene="elaB"
                     /locus_tag="KW011_001618"
     CDS             complement(37263..37568)
                     /gene="elaB"
                     /locus_tag="KW011_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HBH7659976.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(37623..38084)
                     /locus_tag="KW011_001619"
     CDS             complement(37623..38084)
                     /locus_tag="KW011_001619"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH7659977.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            38149..39066
                     /gene="rbn"
                     /locus_tag="KW011_001620"
     CDS             38149..39066
                     /gene="rbn"
                     /locus_tag="KW011_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HBH7659978.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         9581 a        10490 c         9667 g         9328 t
ORIGIN      
        1 aatatcaaag gtaacttcac ttaagccgag atacataaag ccacccgccg cagtggaagg
       61 accatctcca tagcctgctg cgctgttgtt ctcatccact aacacgccgc cgttctggct
      121 gtagctgtca tcaacagaaa tatcaataag atagggagcc gtcgcgctat tggtatatat
      181 cgccccgcca taaccttcgg cagtgttatt tgtaaaggca gtgttattta ttatcgtata
      241 acctggagga tgcttgctgt cgctattatt atcggtaaca tcgattgccc cgccatcgcc
      301 atcactgtaa cttgttgatg tatatgcctg gttgttatca aaaataacat cacttaaata
      361 gacgtcatta ttaatggtat aaattgcgcc acctttgcca tcattagcga tattattgcg
      421 aaacatggcg ttagtgacac ttaaatcgac ggcaccagta tcgttagtac cagaagaata
      481 gattgcgcca ccatagccgc ctgcgacgtt accggaaaaa atgacatcag taagattcag
      541 cgttgagttt tctttagcaa atatggcccc gccgttataa tattctcctg taacggtgtt
      601 attggcaaac agagtcattc cagtttcgtt ttctggtgat aatgtaaatt ccgctccttg
      661 ttgcaaaaat acggccccac cgctggcatt attggtcata tccgaaaaaa ccagccattg
      721 cccatcggcg atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca
      781 actcgctttg atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga
      841 gaaaagagat gcaatcattg acgggagtag agataaatac tccttgcgta gaaagataat
      901 ccgcatatta atctgaccat cattttatat aagacggcat atttaaacgc attatacaat
      961 atgggttatc tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt
     1021 tgatgaataa tatcagtgcg tcataattca agttaataac cttaagggat accagttata
     1081 tttaaactaa attatagtaa tgaataattt tcttataata taaggtaaat taacaaaatg
     1141 gcttcgcatt taacaataac cgaatagaaa acaaccattt cgacatcaac aatcgcttac
     1201 attcgcttat atattgacca caactgatac atcagattat gtgatgactc gtgcttagat
     1261 caatttttgc aatcattaac aaaaagatta ataaaccatc tatatcaatt tatccaacct
     1321 attctgccgt tcaagaaatc gccgaacagt tatttttaac aaatttttct cttcccattg
     1381 actttcccgg acaccttgtc tgacctaagg tgcgcgaaag ccactttttc cttcctgagt
     1441 tatccacaaa gttatgcact tgcaagaggg tcattttcac actatcttgc agtgaatccc
     1501 aaacataccc cctatatata gtgttctaag cagcttccct tactacaggt agtctgcatg
     1561 aaactattgc ggaaagaatt ccaaaaacag gtacgacata catgaatcag aatctgctgg
     1621 tgacaaagcg cgacggtagc acagagcgca tcaatctcga caaaatccat cgcgttctgg
     1681 attgggcggc agaaggactg cataacgttt cgatttccca ggtcgagctg cgctcccaca
     1741 ttcagtttta tgacggtatc aagacctctg acatccacga aaccattatc aaggctgccg
     1801 cagacctgat ctcccgtgat gcgccggatt atcagtatct cgccgcgcgc ctggcgatct
     1861 tccacctgcg taaaaaagcc tacggccagt ttgagccgcc tgcgctgtac gaccacgtgg
     1921 tgaaaatggt cgagatgggc aaatacgata atcatctgct ggaagactac acggaagaag
     1981 agttcaagca gatggacacg tttatcgatc acgaccgtga tatgaccttc tcttatgctg
     2041 ccgttaagca gctggaaggc aaatatctgg tacagaaccg cgtgaccggc gaaatctatg
     2101 agagcgccca gttcctttat attctggttg ccgcgtgcct gttctcgaac tacccgcgtg
     2161 aaacgcgcct gcaatatgtg aagcgttttt acgacgcggt ttccacattt aaaatttcgc
     2221 tgccgacgcc aatcatgtcc ggcgtgcgta ccccgactcg tcagttcagc tcctgcgtac
     2281 tgatcgagtg cggtgacagc ctggattcca tcaacgccac ctccagcgcg attgttaaat
     2341 acgtttccca gcgtgccggg atcggcatca acgccgggcg tattcgtgcg ctgggtagcc
     2401 cgattcgcgg tggtgaagcg ttccataccg gctgcattcc gttctacaaa catttccaga
     2461 cagcggtgaa atcctgctct cagggcggtg tgcgcggcgg tgcggcaacg ctgttctacc
     2521 cgatgtggca tctggaagtg gaaagcctgc tggtgttgaa aaacaaccgt ggtgtggaag
     2581 gcaaccgcgt gcgtcatatg gactacgggg tacaaatcaa caaactgatg tatacccgtc
     2641 tgctgaaagg tgaagatatc accctgttca gcccgtccga cgtaccgggg ctgtacgacg
     2701 cgttcttcgc cgatcaggaa gagtttgaac gtctgtatac caaatatgag aaagacgaca
     2761 gcatccgcaa gcagcgtgtg aaagccgttg agctgttctc gctgatgatg caggaacgtg
     2821 cgtctaccgg tcgtatctat attcagaacg ttgaccactg caatacccat agcccgtttg
     2881 atccggccat cgcgccagtg cgtcagtcta acctgtgcct ggagatagcc ctgccgacca
     2941 aaccgctgaa cgacgtcaac gacgagaacg gtgaaatcgc gctgtgtacg ctgtctgctt
     3001 tcaacctggg cgcaattaat aacctggatg aactggaaga gctggcaatt ctggcggttc
     3061 gtgcacttga cgcgctgctg gattatcagg attacccgat cccggccgcc aaacgtggag
     3121 cgatgggtcg tcgtacgctg ggtattggtg tgatcaactt cgcttactac ctggcgaagc
     3181 acggtaaacg ctactccgac ggcagcgcca acaacctgac gcataaaacc ttcgaagcca
     3241 ttcagtatta cctgctgaaa gcctctaatg agctggcgaa agagcaaggc gcgtgcccgt
     3301 ggtttaacga aaccacttac gcgaaaggga tcctgccgat cgatacctat aagaaagatc
     3361 tggataccat cgctaatgag ccgctgcatt acgactggga agcattgcgt gaatcaatca
     3421 aaacgcacgg cctgcgtaac tctacgcttt ctgccctgat gccgtccgag acctcttcgc
     3481 agatctctaa cgccactaac ggtattgaac cgccgcgcgg ttacgtcagc atcaaagcgt
     3541 cgaaagacgg tattttgcgc caggtggtgc cggactacga gcacctgcac gacgcctatg
     3601 agctgctgtg ggaaatgccg ggtaacgatg gctacctgca actggtaggt atcatgcaga
     3661 aatttatcga tcagtcgatc tctgctaaca ccaactacga tccgtcacgt ttcccgtcag
     3721 gaaaagtgcc gatgcagcag ttgctgaaag acctgctcac cgcctacaaa ttcggggtca
     3781 aaacgctgta ttatcagaac acccgtgacg gcgctgaaga cgcgcaggac gatctggtac
     3841 cgtcaatcca ggacgatggc tgcgaaagcg gcgcatgtaa gatctgatat tgagatgccg
     3901 gatgcggcgt aaacgcctta tccggcctac ggctcggttt gtaggcctga taagacgcgc
     3961 cagcgtcgca tcaggctccg ggtgccggat gcagcgtgaa cgccttatcc ggcctacggc
     4021 tcggatttgt aggcctgata agacgcgcca gcgtcgcatc aggcacagga tgcggcgtaa
     4081 aatgccttat ccggcattaa actcccaaca ggacacactc atggcatata ccaccttttc
     4141 acagacgaaa aatgatcagc tcaaagaacc gatgttcttt ggtcagccgg tcaacgtggc
     4201 tcgctacgat cagcaaaaat atgacatctt cgaaaagctg atcgaaaagc agctctcttt
     4261 cttctggcgt ccggaagaag ttgacgtctc ccgcgaccgt atagattacc aggcgctgcc
     4321 ggagcacgaa aaacacatct ttatcagcaa cctgaaatat cagacgctgc tggattccat
     4381 tcagggtcgt agcccgaacg tggcgctatt gccgcttatt tctattccgg aactggaaac
     4441 ctgggtcgaa acctgggcgt tctcagaaac gattcattcc cgttcctata ctcatatcat
     4501 tcgtaatatc gttaacgatc cgtctgttgt gtttgacgat atcgtcacca acgagcagat
     4561 ccagaaacgt gcggaaggga tctccagcta ttacgatgaa ctgatcgaaa tgaccagcta
     4621 ctggcacctt ctgggcgaag gcacccacac cgttaacggt aaaaccgtaa ccgttagcct
     4681 gcgcgagctg aagaaaaaac tgtatctctg cctgatgagc gttaacgcgc tggaagcgat
     4741 ccgcttctac gtcagctttg cttgttcctt tgcctttgca gaacgcgaac tgatggaagg
     4801 taacgccaag attattcgcc tgattgcccg cgatgaagcc ctgcacctga ccggtactca
     4861 gcatatgctg aatctgctgc gcagcggcgc ggatgatccg gagatggcgg aaattgccga
     4921 agaatgtaag caggagtgct atgacctgtt tgtccaggcg gcacagcaag agaaagactg
     4981 ggcggattat ctgttccgtg acggttcgat gattggtctg aataaagata ttctctgcca
     5041 gtacgttgaa tacatcacca atatccgcat gcaggcagtc ggtttggatc tgccgttcca
     5101 gacgcgctcc aacccgatcc cgtggatcaa cacctggctg gtgtctgata acgtgcaggt
     5161 tgctccgcag gaagtggaag tcagttctta tctggtcggg cagattgacg cggaagtgga
     5221 caccgacgat ttgagtaact tccagctctg atggcccgcg ttaccctgcg catcactggc
     5281 acacaactgc tgtgccagga tgaacaccct tcccttctgg cggcgctgga atcccacaat
     5341 gttgcggttg agtaccagtg ccgcgaaggt tactgcggct cctgtcgcac ccgactggtc
     5401 gcaggtcagg tggactggat tgccgaaccg ttagccttta ttcagccggg ggaaattttg
     5461 ccctgttgtt gccgggcaaa aggcgatatt gaaatcgaga tgtgaattgg ttgtagtgcc
     5521 agatacaacg cttatacgtc ttatctggcc tacaacgatt acatggcgta gtaatacgct
     5581 ttcacctgct cccagtccgc tttggggatt ggctccagat attttccagc tggcggaagt
     5641 catgattaat cgctttattg cgacgcaagc ggcgacgact tttctccagg tcaagaaaac
     5701 cagcttctgc tttaccttct gttttcacat agatatggcg aacataacaa cagccatgct
     5761 gacgattaac gctatgcatt ttcttaaacg ccagcgccac tgctttcaac atggcttgcc
     5821 gtacttcgtc agaatagggc gatactgcat gccgggcata ccagtcagca atgctgatga
     5881 accccgccat atcttcagtc accagcaacg cgcgccattc accctcaatt ttcaccgctt
     5941 caccaaaaac gatcttcggc acgatgacac cggcccgttc cagttcttta attaccgcaa
     6001 cctcacggac aatcgttggt cggccgaacg gataacgtac ggaatgaaac agatgatgcg
     6061 tcatgcgctt tacatacagc tttttgccgt tgcgctcgac gcattgcacc ccgctcatac
     6121 cattacggcg atagttaggc tcttcaaccc agtcgccctc tgttgcccac cagtgattaa
     6181 attcgtcgta ctttgctgaa actgccatac cctatcgcct gtcattttta ttaacgacaa
     6241 tgactatagg tggttacctg aggaaaatct taatgaaacg tgtcgtatta atgaatttct
     6301 tttgccgcta ctagtgtgac accgcttttg cccttcattt gcagcgcata gattccacca
     6361 gccacgacca ctaatagtgc gcccagcaga taaaatgtga atttaatcag gtggatacag
     6421 cgatgaattt tattcgacag ggattaggca tcgccttatt accagagtta acgctgaaaa
     6481 ccattgcagg tgaattgtgt tccgttcctc acgaaccaac cttctatcga cagatttcgt
     6541 tgttggctaa agaaaagccg gtagaaggca gtccactgtt tttactacaa atgtgcatgg
     6601 aacaattagt ggcgattgga aaaatttgat atgagacagg atggcgcatg aacgccatcc
     6661 tgcagtagca ttactcttta ttaaggaatt ttactgcctt atcagggaaa tcagtaaaca
     6721 gcccatttac acccgctttg ttatacagag catcatataa ctgattcaca tcagtagtgt
     6781 attcaggcag tttatccgac cgcacggtat aaggatgcac taccagttta ttctgctgag
     6841 catcttgcac catgccagtg agtttgatat tacccggctg cgatgtttcc tcaatcaaca
     6901 tatggtaatc cggcccgata ccgtcggcat attccgcgac ttgcttcatg gcacctggct
     6961 taaacatcca gtcatagctg taattaaccc agcttccgtc cggctgtttc tgctgcgttt
     7021 cattccagtc ggtataggca atcagctgta ccagattgag atccatgccc attttaggtt
     7081 ccagctcatt cttaatacgc ttcagctcat cagcatcaaa acattgcaaa taaactttat
     7141 cgtccttacc ggtgtaacca tatttcttca gcacttccag cgtttttgcc gcaatatcct
     7201 tcccttcctg atgatggaac cacggcgctt tgatttctgg atagataccg atatttttcc
     7261 cggtagagtg atttaacccc tgaacaaatt caatctcttc ttcaaaggtg tgcccccgga
     7321 agtcggactt acccattggg aaacgccccg gataagtctg tactttttta ccgttttcaa
     7381 tatcgaaacc ttcggtaaat ttcagcgact taatttcatc cagcgtgaaa tctatcgcgt
     7441 agtaacgacc gtctttgcgc gcccgatccg ggaaacgatc ggcaacatca gtaacacgat
     7501 cgagataatg gtcatgcaga acaaccagat ggtcgtcttt ggtcatcacc aaatcctgtt
     7561 ccagataatc cgctccctgc gcatacgcca tcgcttttgc tggcagcgta tgctccggca
     7621 aatatccact ggcaccgcga tgggcgatga ctattttttc gttgctgtcc gccgccattg
     7681 cactgcttcc catgactata gtgctcatca tgatcgccat gctaaggttt ttcagcttca
     7741 atttcatgcc attagcctcc gttgcgtttt tgcagtaatt gttcatggcg acgtttttcg
     7801 ccaatcatca caacaatcaa caagataacc gccagaatgc tgccgccaat cattaccata
     7861 aagccgccat cccagccgaa gaagtccacg gtgtagccaa caatcgcgct cgccgccacc
     7921 gaaccgccca ggtaaccaaa cagcccggta aagcccgctg ccgtacctgc cgcttttttc
     7981 ggtgccagtt ccagcgcatg cagaccgatc agcatcacag gaccgtagat caggaagccg
     8041 ataacaatca tacaaatcat atcgacggtt gggttacctg ccgggttcat ccagtaaacg
     8101 atagtcgcga tggtcaccag tgtcataaag aaaacgccgg ttgccccacg gttgccacgg
     8161 aagactttat ccgacatcca gccgcacagc agagtgcccg gaatacctgc atattcataa
     8221 aggaagtagg cccaggagga tttatccagc gcgaaatgct taacctcttt cagataagtc
     8281 ggtgaccagt cgaggatgcc gtaacgcagc agataaacga acacgttggc gatggcgata
     8341 taccacagca gtttgttcgg cagtacgtac tgcatgaaga tttgcttcgc cgtcagctcc
     8401 tgttccgctt tttcgttata gtcgtccgga taatcatttt tgtactcttc gatcggcggc
     8461 aagccacagg attgcggggt atcgcgcatc atcgcaaagg cgaataatgc caccagaatg
     8521 gcgcagaaag caggcatata gagcgccgca tgccagtcat tgaaccaggc catccccagc
     8581 aggaacagca gcggcggaat accaccaccg acgttgtgcg cacagttcca cactgacaca
     8641 atgccgccac gttctttctg cgaccaccag tgcaccatag tacgaccaca cggcggccac
     8701 cccatcccct ggaaccaacc gcagaggaac aacagtacaa acatcaccgc aatgctcgac
     8761 gtcgcccatg gcacaaagcc cataaacaac atcactgccg ccgccagaat caaacctgcg
     8821 ggcaggaaaa cgcgcggatt cgagcgatcc gataccgaac ccatgatgaa tttcgaaaat
     8881 ccataagcaa tcgagatccc cgaaagggca aaacctaaat caccgcgtga gaatccctgc
     8941 tcaaccagat aaggcatagc aagcgcaaag ttcttacgaa ccaaatagta agccgcatag
     9001 ccaaagaata tccccaggaa aatttgccag cgcaatcgac gataagtcgg atcgatctcc
     9061 gcggcaggta agcgcgcttt gtgtggcgct ggtttaaaaa tactcaacat tgatagcctc
     9121 cgtggcccgt ggtcttattt atgattaaca gcctgattca gtgagagaac ctgccgtttc
     9181 ttgagttgcc gcgatgttaa gaaaacattc ataaattaaa tgtgaattgc cgcacacatt
     9241 attaaataag atttacaaaa tgttcaaaat gacgcatgaa atcacgtttc actttcgaat
     9301 catgagcgaa tatgcgcgaa atcaaacaat tcatgttttt actatggcta aatggtaaaa
     9361 aacgaacttc agagggataa caatgaaaac tcgcgactcg caatcaagtg acgtgattat
     9421 cattggcggc ggcgcaacgg gagccgggat tgcccgcgac tgtgccctgc gcgggctgcg
     9481 cgtgattttg gttgagcgcc acgacatcgc aaccggtgcc accgggcgta accacggcct
     9541 gctacacagc ggtgcgcgct atgcggtaac cgatgcggaa tcggcccgcg aatgcattag
     9601 tgaaaaccag atcctgaaac gcattgcacg tcactgcgtt gaaccaacca acggcctgtt
     9661 tattaccctg ccggaagatg acctctcctt ccaggccact tttattcgcg cctgcgaaga
     9721 agcagggatc agcgcagaag ctatagaccc gcagcaagcg cgcattatcg aacctgccgt
     9781 taacccggca ttgattggcg cggtgaaagt tccggatggc accgttgatc catttcgtct
     9841 gaccgcagca aacatgctgg atgccaaaga acacggtgcc gttatcctta ccgctcatga
     9901 agtcacggga ctgattcgtg aaggcgcgac ggtgtgcggt gttcgtgtac gtaaccatct
     9961 caccggcgaa actcaggccc ttcatgcgcc tgtcgtggtt aatgccgccg ggatctgggg
    10021 gcaacacatt gccgaatatg ccgatctgcg cattcgcatg ttcccggcga aaggatcgct
    10081 gctgatcatg gatcaccgca ttaaccagca tgtgatcaac cgctgccgta aaccttccga
    10141 cgccgatatt ctggtgcctg gcgataccat ttcgctgatt ggcaccacct ctttacgtat
    10201 tgattacaac gagattgacg ataatcgagt gacggcagaa gaggttgata ttctgctgcg
    10261 tgaaggggaa aaactggccc ccgtgatggc gaaaacgcgc attttgcggg cctattctgg
    10321 cgtgcgcccg ctggttgcca gcgatgacga cccgagcgga cgtaacgtca gccgtggcat
    10381 cgtgctgctc gaccatgctg aacgcgatgg tctggacgga tttatcacca tcaccggtgg
    10441 caaactgatg acctatcggc tgatggctga atgggctacc gacgccgtgt gccgcaaact
    10501 gggcaacacg cgcccctgta cgaccgccga tctggcactt cctggttcac aggaacccgc
    10561 tgaagttacc ttgcgtaaag tcatctccct gcctgccccg ctgcgcggtt ctgcggttta
    10621 tcgtcatggc gatcgcacgc ctgcctggct gagcgaaggt cgtctgcacc gtagcctggt
    10681 atgtgagtgc gaagcggtaa cagcgggtga agtgcagtac gcggtagaaa atttaaacgt
    10741 taatagcctg ctggatttac gccgtcgtac ccgtgtgggg atgggtacct gccagggcga
    10801 actctgcgcc tgccgcgctg ccggactgct gcaacgtttt aacgtcacga cgtccgcgca
    10861 atctatcgag caactttcca ccttccttaa cgaacgctgg aaaggcgtgc aacccatcgc
    10921 ctggggagat gcactgcgcg aaagcgaatt tacccgctgg gtttatcagg gactgtgtgg
    10981 tctggagaag gagcagaaag atgcgctttg atactgtcat tatgggcggc ggcctcgccg
    11041 gattactctg tggcctgcaa ctgcaaaaac acggcctgcg ctgtgccatt gtcacgcgtg
    11101 gtcaaagcgc gctgcatttc tcatccggtt cgctggattt gctgagccat ctgcctgatg
    11161 gtcaaccggt gacagacatt cacagtggac tggaatcttt gcgtcagcag gcaccagccc
    11221 atccttacag tcttctcgaa ccacaacgcg tgctcgatct cgcttgccag gcgcaggcat
    11281 tgatcgctga aagcggtgcg caattgcagg gcagcgtgga actggcccat cagcgcgtta
    11341 ccccgctcgg cactttgcgt gcaacctggt taagttcgcc agaagttccc gtctggccgc
    11401 tacccgcgaa gaagatatgt gtagtgggga taagcgggct gatggatttc caggcgcatc
    11461 ttgcggcggc ttcgttgcgc gaactggatc tttcggttga aaccgcagaa atagagctgc
    11521 cggaactgga tgtcctgcgc aataacgcca ccgaatttcg cgcggtgaat atcgcccgtt
    11581 tccttgataa tgaagaaaac tggccgctgt tacttgatgc gcttattcct gtcgccaata
    11641 cctgcgaaat gatcctgatg cccgcctgct tcggcctggc cgatgacaaa ctgtggcgtt
    11701 ggttgaatga aaaactacct tgttcactga tgcttttgcc aacgctgccg ccttccgtgc
    11761 tgggcattcg tctgcaaaac cagttacagc gccagtttgt gcgccagggt ggcgtgtgga
    11821 tgccgggcga tgaagtgaaa aaagtgacct gtaaaaatgg cgtagtgaat gaaatctgga
    11881 cccgcaatca cgccgatatt ccgctacgtc cacgtttcgc ggttctcgcc agcggcagtt
    11941 tctttagtgg cggactggta gcggaacgta acggcattcg cgagccgatt ctcggccttg
    12001 atgtgctaca aaccgccacg cggggtgaat ggtataaggg cgattttttt gcgccgcaac
    12061 cgtggcagca gttcggtgta accactgatg agacgctacg cccgtcgcag gcagggcaaa
    12121 ccattgagaa cctgtttgcc atcggttcgg tgctgggcgg atttgatccc atcgcccagg
    12181 gatgcggcgg cggtgtttgt gccgtcagtg ctttacatgc cgctcaacag attgcccaac
    12241 gcgcaggagg ccaacaatga acgacaccag cttcgaaaac tgcattaagt gcaccgtctg
    12301 caccaccgcc tgcccggtga gccgggtgaa tcccggttat ccagggccaa aacaagccgg
    12361 gccggatggc gagcgtctgc gtttgaaaga tggcgcactg tatgacgagg cgctgaaata
    12421 ttgcatcaac tgcaaacgtt gtgaagtcgc ctgcccgtcc gatgtgaaga ttggcgatat
    12481 tatccagcgc gcgcgggcaa aatatgacac cacgcgcccg tcgctgcgta attttgtgtt
    12541 gagtcatacc gacctgatgg gtagcgtttc cacgccgttc gcaccaatcg tcaacaccgc
    12601 tacctcgctg aaaccggtgc ggcagctgct tgatgcggcg ttaaaaatcg atcatcgtcg
    12661 cacgctaccg aaatactcct tcggcacgtt ccgtcgctgg tatcgcagca tagcggctca
    12721 gcaagcacaa tataaagacc aggtcgcttt ctttcacggc tgcttcgtta actacaacca
    12781 tccgcagtta ggtaaagatt taattaaagt gctcaacgca atgggtatcg gtgtacaact
    12841 gctcagcaaa gaaaaatgct gcggcgtacc gctaatcgcc aacggcttta ccgataaagc
    12901 acgcaaacag gcaattacga atgtagagtc gatccgcgaa gctgtgggag taaaaggcat
    12961 tccggtgatt gccacctcct caacctgtac atttgccctg cgcgacgaat acccggaagt
    13021 gctgaatgtc gacaacaaag gcttgcgcga tcatatcgaa ctggcaaccc gctggctgtg
    13081 gcgcaagctg gacgaaggca aaacgttacc gctgaaaccg ctgccgctga aagtggttta
    13141 tcacactccg tgccatatgg aaaaaatggg ctggacgctc tacaccctgg agctgttgcg
    13201 taaaatcccg gggcttgagt taacggtgct ggattcccag tgctgcggta ttgcgggtac
    13261 ttacggtttc aaaaaagaga actaccccac ctcacaagcc atcggcgcac cactgttccg
    13321 ccagatagaa gaaagcggcg cagatctggt ggtcaccgac tgcgaaacct gtaaatggca
    13381 gattgagatg tccacaagtc ttcgctgcga acatccgatt acgctactgg cccaggcgct
    13441 ggcttaaact cctttctgat gcccggtaag catgtggtta ccgggcattt ttgcgtacac
    13501 gattccgtgc ccaatgtatg cgttgcaacg cagtgaaaat tcctctgaaa acgtctcgca
    13561 aaggctgaaa ctggcagatg tcaaaggcct gggataaccg taatgtcgcg tcatcataaa
    13621 tatcaggtga cggacaacca tgaccgaatc aacaacctcc tccccgcatg atgcggtatt
    13681 taaaaccttt atgttcacac ccgaaaccgc acgggatttt ctcgaaatac atttgccaga
    13741 accactgcgc aagctttgca acctgcaaac cttacgcctg gaacccacta gttttattga
    13801 aaaaagttta cgcgcttact actcggatgt tttgtggtcc gtggaaacca gcgacggtga
    13861 cggctatatc tactgcgtga ttgaacatca aagctctgca gaaaagaata tggcttttcg
    13921 gctaatgcgc tatgccactg ccgccatgca gcgtcacctg gataaaggct atgacagagt
    13981 tccgctggtg gtgccattgc tgttttatca tggcgaaaca tcgccctacc cgtactcact
    14041 taactggctg gatgagtttg acgatccgca acttgcccgg cagttgtaca ccgaagcttt
    14101 tccgttggtg gatatcacca tcgtacctga cgatgagatc atgcaacatc ggcgtatagc
    14161 tctgcttgaa ctgattcaaa agcatattcg cgaccgcgat ttaatcggta tggtcgacag
    14221 gatcaccacg cttttggtta aaggcttcac taatgacagc cagctacaaa cactgtttaa
    14281 ttatctgctg caatgcggcg atacctcccg tttcacccgt tttattgagg agatttccga
    14341 acgttcacca ctacaaaagg agagattgat gactattgct gagcggctac ggcaggaagg
    14401 acaccaaatt ggctggcagg aaggtatgca tgaacaagcc attaaaattg ctttgcgcat
    14461 gctggaacag ggcattgatc gtgaccaggt gctcgcggcc acccagctaa gcgaagccga
    14521 tctggcagcg aataaccact aattaacaca ggcccacagc cgatccccat gggcctttga
    14581 tatcaataac taccttttat gcgtggccca tttttgcctg atttaaacat cgccagtcgt
    14641 tgatccaggg catcgctgta gagcatcgtg tcaacgccaa cagcgacaaa gttcgctccc
    14701 cacgccaggc attgctgcgc catatcagga gccacagcca gaaaaccagc cgctttaccc
    14761 gcagcacgga tccgccgaat gctggtttca ataattcgct gcacttccgg gtgcccggcg
    14821 ttatccgggt agcccaacga cgcagaaaga tccgctggtc caataaacac gccatcaatc
    14881 ccttcgacgt cgaggatctc gtccaggtta tccagtgccg ttttactttc cacctgcact
    14941 aacagacaaa gcgaatcgtt gacttgcgcc atgtaattct caatgcgtcc ccagcgcgca
    15001 gcccgtgcca cactggcccc aacaccacgc tcaccgtagg gaggatagcg cgtggcagac
    15061 gccacctgac gagcctgatc ggcagtatcg accatcggga tcaatagagt ttgcgcgcca
    15121 atatccagaa cttgtttaat cagcgattta ctgccttcca ccggacggat cacgggttgg
    15181 ctggcatagg gcgctaccgc ctgtagctgg tgataaagat cctgaatggt gtttggcgcg
    15241 tgctccccgt caatcagcaa ccagtcataa ccggaagtag cggcaatttc tgccatataa
    15301 gaagtagtcg agcttaacca cagaccaatt tgcacttcgc ccttgcgtaa acgttcttta
    15361 aagggattgg ttaataatgc gttcatcgtg ttccttaacc tatttaatga tgtgccacgt
    15421 cggtctgcgc aacagtacga tttacacgca gagtgaaaat aatcagcgaa ccgatgaccg
    15481 ccaccgctgc cagtgtcagt aatcctgccg catcgctggc aaataacgtt tctgctttca
    15541 cgcgcaggat cggggcaata aacccgccca ctgcaccaaa caggtttaca aaaccaatcc
    15601 ccgcagccag cgccgtaccg gaaagcagtt gtgtcggcat cgtccagaac accggctgca
    15661 cggcaataaa cccgatagct gcaacacaca gcgctacgat cgccattact ggagaaagca
    15721 gcccggacag accaatacca atgcccgccg ccagtaatgt cagcgctgcg acattacgcc
    15781 gttcgccggt tttatcggaa tagcgcggaa taagccaggt cccaaacaag gccgcaaccc
    15841 acggaatggc ggtgaccacc gacgctgtaa agcccacttt tgttcccagc aatgccgcaa
    15901 cctgggtcgg caggaagaaa attaatccgt aaaccgccac ctgaatggtc aggtagataa
    15961 tcgctagttg ccagactcgg ccattacgca gcgcatcgct cagccgagaa gtcactttct
    16021 gttgttcttc acttgccagt tgattgataa gcaacgtttt ttcttgttta ctcagaaaac
    16081 gtgcctgctc cggtgtgtca tcaagccaaa agaatgtgaa taccccagca ccgactgcca
    16141 acaatccttc aatcacaaac atccagaacc agccgggatg ccccataaat ccatgcatct
    16201 ccaacagcgc gccggaaagc ggtgatccca gtgttaacgc cagcggtgcg cccatataga
    16261 acagccccat aatgctggcg cgattacgct gcggaaacca ttgcgaggtg agataaatca
    16321 taccagggaa aaatccggcc tccgcagcac caagcagagt gcgaactatc agaaatttcg
    16381 cttcagtatc ggcccatgcc atggctgccg aaagaaatcc ccacagcagt gttgtcgtac
    16441 caatccaggt tctggccccc agtttgcgca tcaaaagatt cgccggaaca cccagaaacg
    16501 catataccac aaagaaaatg cctgctccca gcgcataagc ttcattactc aacccggtat
    16561 caatctggta ggtctgtttg gcaaaaccga tattcgaacg gtcgagaaac gccagcacat
    16621 acagcgccaa cataaacgga attaaacgca cacggttttt cttcaccacg gcgtcaagca
    16681 aagcggtgct cataaaaagc tccttagaat atgtgggcgt taccgttgtc acagcaacgc
    16741 ccggagtgat tagtggctgt aggggcgttt caaattgcag tcacgattga gttcgacgcc
    16801 aaaaccgggt ttatcaagca ctgatttatg aatacgacca ttcaccggaa ccggctcatt
    16861 gagcagaatc gggtcaaact gcggacgcat cgttgaacaa tccgggctgg tcatcaggaa
    16921 ttcgctgaat ggcgtattgg tgaaggtgat caccgcatgg tgagagtaaa cagacgaacc
    16981 gtgcggcacc accagttgcc ccctggattt ggcgattgcg gcaatttcca ccagcgtggt
    17041 taaaccaccg caccagccaa catccggctg cataatgtcg ataccggttt ctgaaagcgt
    17101 acgaaaagat tgcagtgtgc cgtggtgctc accgctggtg accatcattc cgactggcgc
    17161 gttgcgtttc agttcgcgat aaccttcata ctgctgtggc ggcaggcact cttcgatcca
    17221 tttcaggtta tagggcgcgc aagcgtgggc cagtttggtg gcatagttca cgtcctgact
    17281 catccagcag tcgagcatta accagaaatc ctcaccgcat ttttcacgca tatccgcgac
    17341 cataacggca tctttgcgga tccccgcatc gccatcatgt ggcccccagt gcgtcggcat
    17401 tttgccaccg ataaagccca tctcttttgc cagatccgga cgcgcgcctg tggcgtagaa
    17461 ctgaatctca tcacgaactg cgccgcctaa aagtttataa accggaagcc cgaccacttt
    17521 gccgaacaga tcccacagag ccagatcgac acaggaaatc gtgttcatca ccaggccacc
    17581 agagccggag taatacaggg tggcattgag catttgatca tggatcagtt tgatatcact
    17641 gacacattta ccctcaatga aacggttaag atgtttttcg acaataaaac agcccatttc
    17701 accggctgtc gaaacagcga atccggtctg gccgttttct gcttcgactt caacaatcaa
    17761 cgtgccaaga acgttaatgc caaatgactg gcgtgactgc tcgtaatcgc ggtatttact
    17821 catcggggtg gcaatatgat cgtcaatcca gtgattcgcc ccctggtcgt gataatcacc
    17881 gccgccagca cctttttctg ctgtcgcacc gccagtaaac caggcgcgaa cctgtttgat
    17941 ttttggtagg gtcatgatgt tctccattgt tatgaggcct gtaagtcaaa gggacttttc
    18001 catcccaaca gacgtgaaat atccctggcg caggcaatgg ccttgcccgc cagataatca
    18061 cggtattctt cattgatttg taagcgggta ccgaccaccg agatcgcagc ggtaagctcg
    18121 ttattggcgt taaacaccgg cgcagcgaca caacggacat cggggtaatc ttcgccgttg
    18181 tcatagctcc agccctgacg gcgaatacgc gccagttctt cgtgaagttg ctgtggatga
    18241 gtaatcgttg tgggtgtcgc ctgctcccag accagccctt cgataatact ttgctgtacc
    18301 gctgcaggtt gccaggcaag caggcattta cctattccgg agcgataaag cgaaaggctt
    18361 tttccttcat gggaacgcac gctgatagtg gctgatgact ccacttttaa aatgtaataa
    18421 gcgctgccgt tatcaataat ccccaggtga cataacagcc cggtggtatc catcagttgc
    18481 gtcaaacgcg gtcgagccag ttcccggaga tccatcttgc ttaacgcatg accagaaagc
    18541 tccaccagcc tggtccacaa acaaaaattt tcctggtgat caaggctaag aaaacgctga
    18601 cgcctgagtt cattaagcag caaataggcc gtgcttttag ggattcccaa tgtgtcaatg
    18661 atcgtcgcag cactacaggg gccaatgcgg gcaatcaaat tgagaatatc aatagcccgt
    18721 gtcagggcgg gaactttgct tgattccaac atactggact ccagtcttag atactggaat
    18781 cagtctcgtt gctcaggggg agtaaaattg tgaagcgcat caaatcccgc tcctcgtcca
    18841 gcggactgga gaaattacgc acaaatttgt gaatcagtgc acggattttt tcttattgag
    18901 tacaacaggt tggacaagtt ttgccggagg gggaatgtgc cggtgcagta aaccggcaca
    18961 gagtggcgga atcagacaga taaggtcatg gactcaacaa cctcaatcca gccatgctca
    19021 ctggcgatat cctggccgtt taaccaacgg cgcagcatat tcagtgccat cattgcgcac
    19081 acttcctgac ggatagccag gctgtagcgt gtagtgctga aacgcacacg cagagcgaaa
    19141 gtgccgtctg gcgtggctag cgcaaagttg agatgctcgt tctcgaaacc cgaaacagcc
    19201 agtgccagcc cggcaaaatg gttggcccgc cgttctgtaa tccagtgcgc agtttgcgcc
    19261 agggtttctt cctgtgaagg aaccacttca cacgccagca atggagcacc tgcgcgagaa
    19321 agttgcaaag ccaataaacc accggtgaat tgctcgctca acgtcaggct gaactggcgg
    19381 ttttgcaatt cgcgactgat ctgcgcgggc agtccttcgg tgccttcgaa aatcacgctc
    19441 tgtccggcaa cacgtttaac gtccagccac agtttttcca tcgcctgttg ctcgcttgcc
    19501 ggtccggtga gtttcagttc gatgatcggc attgaggagc gatagcccat tgttacaccc
    19561 ggcggcagtt gtagagtgtc caggctttgt gccagatcgc tttccgaacg accaaaggta
    19621 gttaaacgca gacaaaccgg cgactgtggt aaagaaaaac gctcgcgcag gcgcggcagg
    19681 atttcgtgct cgaccatcac cttaaattct gacggtacgc cgggagtaaa gaacatcagg
    19741 caacgattaa gccgcacggc aaaaccacag gcggtgccta ccgggttatt gataaattca
    19801 gcactggcag gcagctccgc ttgtttacgg ttgctcggtg ccattactcg tccacgttcg
    19861 tggaaatagc gttccatctc tttgagccag gcttcatgca gcaccaggcc ttcacctttt
    19921 gctgtcgcag cggcgagtgc acttaaatca tcactggtcg gtcccagccc gccgttaacg
    19981 atcagcacat cggcgtgctg actacgttcg cgaagaatgg tgactaagtc atcaaggtta
    20041 tcccccaccg tattgcggcg agataatggc aacccctgat gaaagaaaaa atcggccagc
    20101 caggcagcgt tagtgtcaac gatttgcccg tgtaacactt catccccggt ggataacatt
    20161 tccactttta acatggttat ctcctgcttt tgttatcgaa tcactataac gcaaagggag
    20221 ataaggcgta gtaagaaact gaatggcgcg acagaatgcc gcgccggaag ggatcagaaa
    20281 ctggcgttaa ccccaacata cgggccgtca gccacggcgt tgtcgcggtt accgtcttta
    20341 cccgacagat tcaggtagcg ataacccgcc tcaatactga ctggatgcat aatggtgtaa
    20401 cgcgcgccag cattcgcttc ctcataactt ttaataccgc tggagagtga atccggagag
    20461 tagtaatact cgccaaacaa acggaagctg ttgccaattt tccactgcaa accacctcct
    20521 accgctgcgg cataaccttc atcgccgtaa ttcgggttgg tgtaaacgcc ttttccgcca
    20581 acggtcgcca ttaacggccc gagaggcaga ttcaacccca gccccacgcc agcgacgtca
    20641 ccgtcgtcgt cgttatgtgt ccagttaccg cttaacgcca ggcccgtcga ttcagtccca
    20701 aaaccgacac caatattggt gtattcttta cccgcctgac cgctgatgct cattgcattg
    20761 accgatgcag aaaccaacag cattccggcc agacctgcta gcgcaatttt tttcattatc
    20821 ccctttccca caagcaatta atcaaaattc ccaaagctgc gggattgtac tgcctggcac
    20881 agaggattca atgagcgaaa aaagggatct agtcacttat tgtaactaaa tacttcttaa
    20941 ataattaaac gccgaaaaaa taaacacact ttaccgatac acacaaaaat aataataagt
    21001 gcagttatca tcgcaaaaga gattactgtc actttcacat atttctgagt taccgtattc
    21061 tcatggtttc tttttattta taaggaatac ccctgtgcga caacggacta ttgtatgccc
    21121 tttgattcaa aatgatggtg cttatttgct gtgcaaaatg gccgacgatc gcggcgtttt
    21181 ccccggtcaa tgggcgcttt cgggtggcgg cgtggagtct ggcgaacgaa ttgaagaggc
    21241 actacgccgc gaaattcgcg aagaactggg agaacagctg cttttgacag aaatcacgcc
    21301 gtggaccttc agcgatgata ttcgcaccaa gacgtatgca gatggtcgca aggaagagat
    21361 ttatatgatt tacctgattt ttgactgcgt ttctgccaac cgagaagtga aaataaacga
    21421 agagtttcag gactacgcgt gggtaaaacc tgaagatctg gtgcattatg atttaaatgt
    21481 tgccacccga aaaacgttac gtttgaaagg tcttctttaa caatgatgac agccatcact
    21541 ggctgtcaga ttgaaaattt agtggttaac gaattcccca tccagataaa ctttgccttt
    21601 ttccacatgc atgactaaac catccagata atcaggttta aatgtcgcat cacgcttatt
    21661 tttagcaata tatgtcaggc aatgattatg ggtgaaaata acgatatttt tatcaggcgc
    21721 tttgctttgt aagtccttaa ttgcactata aatctcatta ccgcactgaa gaagtcgttt
    21781 atctaccgtc agttttttac ccgcggaaaa ccaggtcgcc gactgaatgg tccggacggt
    21841 attactggaa taaagatcga aatcagggat gtcagcgcta aaagcgttgc ccagttcacg
    21901 ggcatcctgg gtacctttaa ccgtaatacc tgttttatct gacaagcatt gattggttga
    21961 acgatcgcaa cgttcagcat ggcgaaacaa aacgacaacc gggtgctgct gtgccagtct
    22021 ggccaatgtt ttattgtcga tacgtggcaa accattgcta ctccaggcgg catgagtacc
    22081 cagtccggca attgcagcga gcgccagtaa aatgatgaaa tatttttttg acttcaatga
    22141 tgagcggcaa aaagctaaca cgtaaactcc acctatagac aagcgcaacc agacaattac
    22201 cgtgaaattg agcgacattt ctggcgataa ttagcagttg gtgtaatatt aaaaatccta
    22261 cgatgtcggc aaaatgcctc aaaattttgc caaatgcaaa gcctaaataa gaaaaaatat
    22321 aaaaatttca atatttacgt ctaatattag tttcttaagg ttaagttaat attctatcct
    22381 taaaatttcg ctccaaatgg caaaatatac acaacactct ttatagcaaa tataagtgga
    22441 caggtattca atggcggaag gaaaagcaat gtcagaattt ttgccttttt cgcgaccagc
    22501 aatgggcgtg gaggaactcg ctgcagttaa agaggttctc gaatccggtt ggatcacaac
    22561 cggtccgaag aatcaggcgc ttgagcaagc cttttgccag ttgacgggaa atcagcatgc
    22621 catcgcggtc agttcagcca ccgccggaat gcatatcacg ctaatggcgt tggaaattgg
    22681 caagggcgat gaagtgatta cgccttccct gacctgggtt tcaaccctca atatgatttc
    22741 cttgttgggt gcaacgccgg taatggtgga tgtcgaccgc gatacgctga tggtcacgcc
    22801 tgaagcgatc gagtcagcca ttacgccacg cactaaagcc atcattccgg tgcattatgc
    22861 cggtgcgcca gcagatattg acgccattcg cgccattggc gaacgttacg gcatcgcagt
    22921 tatcgaagat gctgcccatg ccgtcggtac gtattacaaa gggcgacata ttggcgcaaa
    22981 aggtaccgct attttttcat ttcatgccat taaaaatatt acctgtgcgg aaggtggcct
    23041 gattgtaact gataatgaaa accttgcccg ccagctacgg atgctgaaat ttcacggtct
    23101 gggtgtcgat gcctatgaca gacaaacctg gggccgtgca ccgcaggctg aagtcttaac
    23161 accgggctat aagtacaatc tgaccgatat taacgccgcg attgccctga cacagttagc
    23221 aaaattagag cacctcaaca cccgtcggcg cgaaattgcc cagcaatatc agcaagcact
    23281 ggcagctctc ccctttcagc cattaagcct tcccgcctgg ccgcacgttc acgcctggca
    23341 tctgtttatt attcgtgtcg atgaacaacg ttgtggtatc agtcgcgatg cgttgatgga
    23401 agcgttaaaa gaaagaggca ttggtaccgg gttacatttc cgcgccgctc acacacaaaa
    23461 atattatcgc gagcgttttc ccacgctgtc gttaccgaat accgaatgga atagcgaacg
    23521 catctgttct ttgccgctgt tcccggatat gactaccgcc gatgccgacc gtgtcatcac
    23581 agcccttcag caactcgcag gacaataagc catgtttgaa atccaccctg ttaagaaagt
    23641 ctcggtggtt attcccgttt ataacgagca ggaaagctta ccggaattaa tcaggcgcac
    23701 caccacagcc tgtgaatcgt tggggaaaga gtatgagatc ctgctgattg atgacggcag
    23761 tagcgataat tccgcgcata tgctggtcga agcctcacaa gcggagaaca gccatattgt
    23821 gtctattttg cttaaccgca attacgggca acattcagcg attatggcgg gattcagtca
    23881 cgttactggc gacttaatta ttacccttga tgccgatctc cagaatccgc cagaagaaat
    23941 cccccgcctg gtggcaaaag ccgatgaagg ttacgacgtg gtagggactg tacgccagaa
    24001 ccgccaggac agctggtttc gtaaaacagc ttcgaagatg attaacaggc ttattcagcg
    24061 caccaccggc aaagcgatgg gtgactacgg ttgtatgctg cgcgcctatc gccgtcatat
    24121 tgtcgatgcg atgttgcact gccatgaacg tagcaccttt atcccgattc tggcgaatat
    24181 cttcgcccgc cgtgccattg aaattccagt acatcatgcc gagcgtgagt ttggtgaatc
    24241 caaatacagt tttatgcgcc tgattaattt gatgtacgac ctggtgacct gccttaccac
    24301 aacgccgcta cgtatgctga gtctgctcgg cagcattatt gcgattggag gttttagcat
    24361 tgcggtgctg ctggtgattt tacgcctgac cttcggacca caatgggcgg cagaaggcgt
    24421 ctttatgcta tttgccgtgc tgtttacttt tattggcgct cagtttatcg gcatgggatt
    24481 gctcggtgaa tatatcggca ggatctacac cgatgtccgc gcccgccccc gctattttgt
    24541 tcagcaagtt atccgtccat ccagcaagga aaatgaataa tgaaaaccgt cgtttttgcc
    24601 taccacgata tgggatgcct cggtattgaa gctctgctgg ctgccggtta cgaaattagc
    24661 gccattttta cccataccga taatcccgct gaaaaagcct tttatggttc ggtggctcgt
    24721 ctggcggcgg aaagaggcat tccagtttat gcgccggata acgttaatca tccgctgtgg
    24781 gtggaacgca ttgcccaact ctcaccagag gtgattttct ctttttatta tcgccatctt
    24841 atttgcgacg agattttgca gctcgctccc gcaggtgcat ttaatctgca cggttcgctg
    24901 ttaccaaaat atcgtggtcg cgcgccgctg aactgggtgc tggtgaacgg tgaaacggaa
    24961 acgggcgtta cattgcaccg aatggtaaaa cgtgccgatg ctggggccat tgtggcgcaa
    25021 ctgcgcgttg ccattgcgcc agacgatatc gctattacgc tgcatcataa attgtgccat
    25081 gccgcgcgcc agctactgga acagacatta cccgccatta aacacggtaa tattctggaa
    25141 attgcccagc gcgaaaacga agccacctgt tttggtcaca gaacgccaga tgacagtttc
    25201 cttgaatggc ataaaccggc atccgtactg cacaacatgg tacgtgccgt tgccgatccg
    25261 tggccgggtg ccttcagcta tgttggcaat cagaaattca ccgtctggtc gtcgcgtgtt
    25321 catcctcatg ccagcaaagc acagccgggg agcgtgattt ctgttgcgcc actgctgatt
    25381 gcctgtggcg atggcgcgct ggaaatcgtc accggacagg cgggcgacgg cattactatg
    25441 cagggctcgc aattagcgca gatgctgggc ctggtgcaag gttcacgctt gaatagccag
    25501 cctgcctgca ccgcccgacg ccgtacccgg gtactcatcc tcggggtgaa tggctttatt
    25561 ggcaaccatc tgacagaacg cctgctgcgc gaagatcatt atgaagttta cggtctggat
    25621 attggcagcg atgcgataag ccgttttctg aatcatccgc attttcactt tgtcgaaggc
    25681 gatatcagta ttcattccga atggattgag tatcacgtca aaaaatgtga tgtcgtcttg
    25741 ccgctggtgg cgatagccac gccgattgaa tatacccgca acccgctgcg cgtatttgaa
    25801 ctcgattttg aagagaatct gcgcattatc cgctactgcg ttaagtaccg taagcgaatc
    25861 atcttcccgt caacttcaga agtttatggg atgtgtagcg ataaatactt cgatgaggac
    25921 cattctaatt taatcgtcgg cccggtgaat aaaccacgct ggatttattc ggtgtcaaaa
    25981 caattacttg atcgggtgat ctgggcctat ggcgaaaaag agggtttaca gttcaccctc
    26041 ttccgcccgt ttaactggat gggaccacga ctggataacc ttaatgcagc gcgaattggc
    26101 agctcccgcg ctattacgca actcattctc aatctggtag aaggttcacc aattaagcta
    26161 attgatggcg gaaaacaaaa acgctgcttt actgatatcc gggacggtat cgaggcgttg
    26221 taccgcatta tcgaaaatgc gggaaatcgc tgcgacggcg aaattatcaa cattggcaat
    26281 cctgagaacg aagcgagcat tgaggaactg ggcgagatgc tgctggcgag cttcgaaaaa
    26341 catccgctgc gccatcactt cccaccgttt gcgggctttc gtgttgtcga aagtagcagc
    26401 tactacggca aaggatatca ggacgtagag catcgtaaac cgagcatccg caatgcccgc
    26461 cgctgcctgg actgggaacc gaaaattgat atgcaggaaa ccatcgacga aacgctggat
    26521 ttcttcctgc gcaccgttga tcttacggat aaaccatcat gaccaaagtc ggcttacgca
    26581 ttgatgtcga tacctttcgt ggcacccgtg aaggcgtgcc acgtctgctg gaaatcttga
    26641 gtaagcataa tattcaggcc agcatttttt tcagcgtcgg cccggacaat atgggccgcc
    26701 atctctggcg actggtgaag ccacagtttt tgtggaagat gctgcgctca aacgcggcat
    26761 cgctttatgg ctgggatatt ttactggcag gtacggcctg gccaggtaaa gagattggtc
    26821 atgccaatgc cgatatcatt cgtgaagcgg ctaaacatca cgaagtcggc ctgcacgcct
    26881 gggatcacca tgcctggcaa gcccgtagcg gtaactggga tcggcaaaca atgatcgacg
    26941 atattgcacg cggtcttcgc actctggaag agattatcgg tcaaccggta acctgttctg
    27001 ccgctgcggg ctggcgtgcc gaccagaagg tgatcgaagc aaaagaagcg ttccatttgc
    27061 gctacaacag cgattgtcgt ggggccatgc cgttccgtcc actgctcgaa tcaggaaacc
    27121 ctggcactgc gcaaattccg gtgaccttac ccacctggga tgaagtgatt ggtcgggatg
    27181 tgaaagcaga agattttaac ggttggttac tcaaccgcat cctgcgagat aaaggcacgc
    27241 cggtttatac cattcatgca gaagttgaag gctgcgctta tcagcataat tttgtggatc
    27301 tcctcaaacg cgcagctcag gaaggcgtga cattttgccc tttaagcgaa ctgttatcag
    27361 agacgttgcc gctcggacaa gttgttcgcg gaaatattgc cggacgtgaa ggctggctgg
    27421 gttgccaaca aattgcgggt agtcgctgat gaaatcggta cgttacctta tcggcctctt
    27481 cgcgtttatt gcctgctatt acctgttacc gatcagcacg cgtctgctct ggcaacccga
    27541 tgaaacgcgt tatgcggaaa tcagtcgaga aatgctggca tccggcgact ggattgtgcc
    27601 ccatctgtta gggctacgtt atttcgaaaa acccattgcc ggatactgga ttaacagcat
    27661 tgggcaatgg ctatttggcg cgaataactt tggtgtgcgg gcaggcgtta tctttgcgac
    27721 cctgttaact gccgcgctgg tgacctggtt tactctgcgc ttatggcgcg ataaacgtct
    27781 ggctctactc gccacagtaa tttatctctc attgtttatt gtctatgcca tcggcactta
    27841 tgccgtgctc gatccgttta ttgcattctg gctggtggcg ggaatgtgca gcttctggct
    27901 ggcaatgcag gcacagacgt ggaaaggcaa aagcgcagga tttttactgc tgggaatcac
    27961 ctgcggcatg ggggtgatga ccaaaggttt tctcgccctt gccgtgccgg tattaagcgt
    28021 gctgccatgg gtagcgacgc aaaaacgctg gaaagatctc tttatttacg gctggctggc
    28081 ggttatcagt tgcgtactga cggttctccc ctggggactg gcgatagcgc agcgggagcc
    28141 tgacttctgg cactattttt tctgggttga gcatattcaa cgctttgcac tggatgatgc
    28201 ccaacataga gctccgttct ggtactacgt gccggtcatc attgccggta gcctgccgtg
    28261 gctgggatta ctccccggtg cactgtacac aggctggaaa aaccgcaagc attccgcaac
    28321 cgtctatttg ttgagctgga cgataatgcc gctgctgttt ttctccgtcg ctaaaggtaa
    28381 attgcccacc tatattcttt cctgctttgc atctctggca atgctgatgg cgcattacgc
    28441 tttgctggca gcaaaaaata atcctctggc gctgcggatt aatggctgga ttaacatcgc
    28501 ttttggcttc actggcatta ttgccacatt tgtggtctcc ccgtggggac caatgaacac
    28561 gccggtgtgg caaaccttcg agagctataa agtcttttgt gcctggtcga ttttttcgct
    28621 atgggcattt ttcggctggt acaccttaac aaacgtcgaa aagacctggt cttttgccgc
    28681 gctttgcccg ctggggctgg cgttgctggt aggattttca attcctgaca gagtcatgga
    28741 aggaaaacat ccgcaatttt ttgtcgagat gacacaagaa tcacttcagc caagccgcta
    28801 tattcttact gatagcgtcg gtgttgccgc aggtctggca tggagcctgc aacgcgatga
    28861 catcatcatg tatcgccaga caggtgagtt gaaatacggc cttaattatc cggatgcgaa
    28921 agggagattt gtcagcggtg atgagttcgc aaactggctt aatcaacatc gtcaggaggg
    28981 gattattaca ctcgtgcttt cggttgaccg cgatgaagat atcaacagtc tcgccattcc
    29041 gcccgcagat gccatcgatc gtcaggagcg tctggtgctg attcagtatc gtcccaaatg
    29101 atctggctaa cattagtctt tgccagcttg cttagcgttg ccgggcagtt gtgtcagaaa
    29161 caggcaacct gctttgtggc gataaacaaa cggcgcaaac atatcgtgct gtggctggga
    29221 ctggcgctgg cttgtcttgg tcttgccatg gtgctctggc tgctggtctt gcagaacgta
    29281 ccggtaggca ttgcctaccc gatgttaagt ctgaattttg tctgggtgac gctggctgca
    29341 gtaaaactgt ggcacgaacc ggtatcgccg cgtcactggt gtggggtggc gttcattatt
    29401 ggcggcattg tgatcctcgg gagtacggtg taatgggcct gatgtgggga ttattcagcg
    29461 tcattattgc ctcagttgcg caattaagcc tgggttttgc ggcgagtcat ctgccgccga
    29521 tgacgcactt atgggatttt atcgcggctc tgttagcttt tggcctggat gcccggatac
    29581 tgttactcgg gctgctgggt tatctgcttt ccgttttttg ttggtataaa acgctacata
    29641 aacttgcgct aagcaaagcc tatgccttgc tcagtatgag ttatgtgctg gtatggattg
    29701 cttcaatggt acttcccggc tgggaaggga ctttttcgct gaaagcacta ctgggagtag
    29761 cctgtattat gagcgggttg atgctgattt tcctgcccac gacaaaacaa cgttactgag
    29821 ttttccctgc cactttacac tgccgttccc actcatcggg gctataactg cttgccgaga
    29881 ggactttcac cgtatgcagc ttttcacgat taatacaata aagcgcattc tgcaaaggcg
    29941 agagtaaatc ccccactttc acggcgaagt cactcttcac ttcggcgatc atttttatcg
    30001 cgccgccagc atcgcagagc ataagtacat gtctgttatc ttgtttattg cagcacgatt
    30061 ttttgaccag ccattccatt gcattatcct gtttgctaag agttttcaca tcaatttaca
    30121 gattaacaga taaacctatt atcaacggca atggaatggc aaaattgcga agtgaaacta
    30181 atgatttctt aatcctggcg ttgcacccac tcttttaacg cctgacgtga aattttaata
    30241 ccgccgtttt tcagctccgg cggtaaagtt agccagcgca ccggttgttg aaaacgagcc
    30301 agcttatctt tcacccattc gccaagatca acggtttgct ggtcatactc caccaccgct
    30361 accggtcgat gaccaaactc cttgtcggca acggggacga taaacacctg caaaaccgca
    30421 ggatgtgcag caattacgcg ctcgacttct tccggctgaa taccctctcc gccactgaag
    30481 aacagattgt ctaaacgtcc gacaatggtc agcttgccat tatgcatctc accgcgatcg
    30541 cgcgtagcgt accagccttc gtcattaacc agtgaaacca gttgcccgtt acgccagtaa
    30601 ccttctgcca tactggcagc ccgcagccac acttcattat taacgatttt cacttcccga
    30661 cccggcagcg gcgaaccaac gtctgccagg ccgtcggctt ctttcgcaca caccgtggag
    30721 gcaaactcgg tcagaccata gccgcaaaag caacgaatcc cctgctcgcg cgcctgttcc
    30781 gtcaactcga ccgggatagc cgcgccgcca agtaacaccg ctttcaggga aacggaactg
    30841 cggttaacca gcaaacgcca gagttgtgtt ggcaccagtg aagcgtgagt acagcctgcc
    30901 agcatttgct ccaatggctg tttatcacgc accgtcatcc gcgcaccagc gtataaccag
    30961 cgccacataa ttccctgacc ggagacgtga aacagcggta aagagagcaa ccagtcatcg
    31021 tgatcgccaa acggaatcag cgataacaca ccttgcgcac tggcaagatg ggcctgataa
    31081 gtatggacag cggctttcgg caaaccggta gaacccgagg tcaacgtcat tgagcacaga
    31141 cgcgtcggct gccacgtagc ggcatgtgcg ccttcaaccc gctgaatgtg cagcgacgtt
    31201 aatgccggaa acgtgttttc cccatccggc accagcgcaa attgcagcgt cagattgggc
    31261 agcaattctt caagcaacgg ttgcggcagc tgagggttca cgggcaacac ccgcgccccg
    31321 cattgcagta acgccagcca ggcgagcagc gtttgcggcg tattccacgc ccgcaacatc
    31381 acgccgctgc cctcaaccac accctgcgcc gcaaagcccg tagctaactc atcgacgcga
    31441 gcacaaagct cgcgccagtt gagttgctcg tcattaagac gtaaggcgat ggcttctccc
    31501 cgcacttgcc gccagtgacg ccacggccag tcagagaaga tcataacaac tgctccagta
    31561 catcagcgtc cactaacggc agtgggctcc ccggccagcg acgaacctgc tgcgcctgca
    31621 tcagatccag cgtgtccagt cccggaatgg tgtctggcgt taaccaggca gcaatccgcg
    31681 ccagttgcgt tagacctaag ctcgactcaa tggaagaact gatcaccgcc gtcagcccca
    31741 gcgcatgcgc cgcctgtacc tgctcgcgta ctttttccag actgcccgtg agcgtgggtt
    31801 tgataactac cgcgcgcacg ccctcttcag ccacaaaggc aaaatccggc tcgcgcaggc
    31861 tttcatccca ggcaatggca atgccggttt cacgggcaaa cgctcgcgaa tcatcgcggg
    31921 ttttgcacgg ctcttcgaga aacgcgatgc ggtggcgata atccgggtta acatatttgg
    31981 caaactgctg acctttcagc ggtgtccagg cgcgatttgc gtcaagacgc aaatgcagat
    32041 ccggaattgc ctccagcaac agattaacca ccatgccgtc gcgcacggct tcgtacaatc
    32101 ccactttgac cttcgccact ttctcgcctg gcatatcagc aagtttgagg atcagatcgt
    32161 caggatcgcc attacacagt ggtgccgcac ggtaattggc cgcctgcggg agcgtatctg
    32221 ccagctccgc caatgcacag cttacgccaa aggcaacgga aggcatctgc ggtagctcgc
    32281 catcgcctgc cagccagtta tttacccaag caagcagcac aatttgcgcc tcttcccagg
    32341 tttcctgact gaagcccggc agtggggaga tctcccccca cccttcgcgc tcgccttcac
    32401 gcaggcaaac atacagcccg tcgcgggttt ttaaccgcct gtcgcgcaga accatccccg
    32461 cgtccatggg gatctgccag cggtatacct gcgcgctacg cattacggat tccgtttaaa
    32521 tttgctgaaa tcaggctgac gtttctggtt gaaagcgttg cgaccttcct gaccttcttc
    32581 cgtcatgtag aacagcatgg tggcgttgcc cgccagctcc tgcagacccg cctgaccgtc
    32641 acagtcggcg ttcagcgcag ctttcaggca gcgcagcgcc atcgggctgt tttgcagcat
    32701 ttcgcggcac caacggacgg tttctttttc cagatccgcc agcggtacca cggtgttgac
    32761 aaggcccata tccagcgcct gttttgcatc gtactgacgg cacaggaacc agatttcacg
    32821 cgcttttttc tgtccgacga tgcgagccat gtaggaagcg ccccagccgc cgtcgaagga
    32881 accgactttc gggccagtct gaccgaagat ggcattatct gccgcgatag tcaggtcgca
    32941 catcatgtgc agaacgtgac caccgccgat ggagtagcca gccaccatcg cgacaaccgg
    33001 tttcggacag gtacggatct gacgctggaa gtccagcacg ttcaggtgat gtacgccgga
    33061 atcatcttta tagccgccgt aatcaccacg cactttctgg tcaccaccgg agcagaacgc
    33121 tttatcgcct gcgccagtca gaatgatcac gccgacgttg tcgtcataac gcgcatctgc
    33181 cagtgcctgg atcatctctt ttaccgtcag aggacggaag gcattgcgca cctgcggacg
    33241 attaatggtg atttttgcga taccgtcggt ggatttttca taacgaatgt cctcaaaacc
    33301 ttcggagcag tcgtgccatt caaccggtgc gtaaagcatt gcttcatcag gataaatcat
    33361 attgtgtcct ttagtcagaa acgcaagatc tgcgccagac ttgcgataac gccagcggga
    33421 ttttcccgat gcgcgttatg tccggcgcga ggaatgacat ggcagtcggc agccagttcc
    33481 gccgccaagg cgcggaattt gctgtcacgt tcaccacata aataataaaa cgcaaatgtg
    33541 cgggcgctaa ggttagcacg taaatcaggc tggacggcga gagaagtcgc ctccagcatg
    33601 gcggcaaggg ttgcgccatt attgttactg cgcagcgcca ccagctcccg gcgttgatcg
    33661 tcattgagcg aggcaaaaac aggctgttga taccagtcgg caaataccgc cgttaacggt
    33721 tctgtgcgaa aacgctgcgc ccattggcga tcggaacgct gacgttccgc gcgttgttca
    33781 gcattttgca acccaggatg cccgccttcg acaacaaccc cacaaagccc cggcatcccc
    33841 tggcaagccg ccatcatcgc cacccgtccc ccaagcgagt accccaccaa ccagaagtta
    33901 aggatgttgt aactaaccag ggttttacgc agtaagtcgg tgacatcatc aaatccatcg
    33961 acgctaatcg ccgccgaacc accgtgacct gggagataaa catacaaccg tgagtagtcg
    34021 gcaaacgcct cgcccacttc ttgccattcg tggcaatcgc cggaaaaacc gtgcaaaaac
    34081 accagccagg gtaaacctgg tttgccgtgt tttgcctgcg cgtgcaggat cataaatggc
    34141 ttacctgcgc cagaagttgc tggagcgttt gcgcaccatc ggtgtcgtta accaccattt
    34201 caattaccgt ggtggttggc gtgcgccagg catcggcaag tgccgtttca agttcctgcc
    34261 agttttgcgg acgatgatat ttcagctcga acatcgcggc ggcgtgctca aaatggacgt
    34321 tttgcggcat cagatagaaa cgctcgcgct cgcttttcgg cgttggcagc agcgagaaaa
    34381 tttgcccgcc gttgttgttc accacaatta atatcagcgg cgcggaaacc tgacgcaata
    34441 acgccagcgc gttgagatcg taaagtgcgg agagatcgcc cacaatcgcc agcgtcggtt
    34501 tgccgcttgc ccgctgaacg ccggcggcag tcgagagcag cccgtcgata ccgctggccc
    34561 cacggttgct gtacaccggg taacctgccg gaagttgcga aagcgcatca atcagacgta
    34621 ccaccaggct gttaccgaca aacaattgcc cctgttcagg cagatagtcg ctgatgcgat
    34681 gcgccagttg cgcttcgcca aacgcatcgc gacgggcaat aaccgcctgc attgcctgtt
    34741 ccgccaggcg cgggatttca acgcaccagg gctggcgttt ttctgccgga tgcagctcca
    34801 gccagtcggc aatattggca attaagcggc gtccacgatg gtgcgccgga tcaagccgcc
    34861 cttcgatgtc atccacaatc cagtactctt cgggttcaca gcttgcctgc cattgcagga
    34921 gccgtttgcc cgtcaggctg cttcccagtt gcaccacaat ttgcgcctgt tgcagttcgc
    34981 tggtcgcttt ggcattgcct aaccagagat cggcacacgg cagcggctgt ccggtttgcg
    35041 acagcacgtc gccaatcagc ggccagccaa gagtttgcgc ccacagtgca acttttttgc
    35101 cctcttccgc actcatgcgc ccggcaacca ccacgccgcg tttttgtcgc cagaagaacc
    35161 agtcgcgctg tttttcactt tccagacgag gcgcttcacg cagccacggt ttgtcgtcct
    35221 gccaccagtc gcccagccgc tgttgccagc taagcccggt gtcgtccatt tcgccataca
    35281 gcggttcggc aaacgggcag ttgatatgga ctccaccaga atgaagcgta ccgagagcat
    35341 ggtcgatggt agaaaccagc caacgtgcgg ggatatcctg ggtcgggcgc ggcaatgaaa
    35401 tactgtgcgt ggggtgagag gcgaacattc ccggctgacg aatcgcctga ttcgcgccgc
    35461 agtcaatcag ctccggcggg cgatcggcgg ttaacaggat cagtttttct ccggttaacc
    35521 cagcttcaat cagtgccgga tagagatttg ctaccgccgt accggaggtc acaatcaccg
    35581 ccaccggctg cttgctgact ttcgccagcc ccagcgccag atgccccaac ccacgctcat
    35641 cgaaatgggt gtggtgaatg aatgcggaat tctccgccgc cgctaacgtt aacggtgtag
    35701 aacgcgagcc aggggcgata cagatgtgtc tgacgccgtg acgcgttaat gcttccagaa
    35761 tgaccgccgc ccagcgtcgg ttaaatgcgc ttactgacat gagtttgtcc ggtatcaata
    35821 ttgcggctaa gtataaggag ctacaaaaat agattattga tatgaatcgg taatgatgcg
    35881 actcattact attccatctg taataaagta cgcagccctg ccgctttgtt gtcgatttcc
    35941 tgccactctt gctcggggtc ggaaccacgg acaatgcccg cgccagcata taatcgcacg
    36001 acattgccgc taatttttgc tgagcgcagg gaaacgcaga attcgctttg ctgtaatgag
    36061 agatagcccg cagaaccggc gtaccattct cgggtgaacg gttcgtgacg ggcgataaac
    36121 tgtcgcgcca gatcgcgcgg taagccagca actgctgccg ttggctgcaa ctgatgtaaa
    36181 cagatcacat tatccgcttt gttgagtgaa gtccagatac agcggcgaag atgctgcact
    36241 ttacgcagac gcagtacctg cggcggtaaa acatccagcg tctgggtatc ggcctgtaac
    36301 cgttggcaga tatcttccac caccagcata ttctcgcgct ggtttttatc atccgccatc
    36361 agccactcac ctaactgctg cgcctgctta tcatcaggat gatttgctac cgttcccgcc
    36421 agcgcttcag tacgcagcgc tttgtcacgc cgccgccata accgttccgg tgaagagcca
    36481 agaaaagcat tttcgccatc aaaggccatg taaaaatggt agcaattcag attcagtcga
    36541 cgactggcag ccatcatcgc cgccgcgttg accggacttg cgaaatgcag gtcagttgcc
    36601 cgagcgagca ccactttgtc gagctcacct tcggcgatgg ttttcgttgc cagttcgatt
    36661 aattgcgtcc agcccgtttt gtccggccag tgttgttctc gcgtggtggt taaatgtaac
    36721 ccaggcaagg gcttgatact caccagtgtg gcgataaatt ctcttgcctt aatcgcatcg
    36781 tgctgaaggg agctttcgct gaatagcgtc agccgcagcg tggctttacc gccacagcgt
    36841 cgccattcca ggcgtggtaa aagtaaattg ccctgcgacg ggtcaaacgc attcagcccc
    36901 caaatgcgta agtcggcgtg ttccgggtgc tggcgaagaa aacgttgtgc ctggtccaac
    36961 gacgtaaaac gggtaatcgc gcccaggacg gcagcttctt catcaccatt acgttgttgc
    37021 cagtagaatt gcgggtatgt ttgctgactg gccagccagc tcaaggcatc aaaagcgtct
    37081 ttgagaggga aaggaatatc gataacccga atgccgggtg tcgccggaat ctcttgcgac
    37141 aaatggcgca gtagattttc cagcgccgta gtaagtgatt gcacgcggac ctctccctgt
    37201 taaaagcctc acattatacg gggtactaca aaaaaaatgc agtaccccgg tgtagggagg
    37261 ttttaacggc gtgccagcaa cagtcctagt accagcccaa cggccgcgcc cacaccaatt
    37321 ccttgccagg gtttttcgtg gacgtagtca tcagcacgat aaactgcctg cttcgcccga
    37381 tagtaataac tgtctgaagc ctggctaacc cgttttttca catcatccag cgctttttct
    37441 gcacgcgctt tcagctcaac atatttctga tcggcgggat cgccagagga gcggagcacc
    37501 tcttccagtg tttcactaag cagcgtcagg tcgtcatcga tacgtgtatc accaaactga
    37561 ttagacattc tcgttctcca tttgcgtaaa acctgtcact aactatagac aagggttgtt
    37621 gattacgcct gaattacctc gcgtgccatc ccaatgtgtg ggataccatc ttcttcatag
    37681 acctccgtca cagggataaa accgaaactc tggtagaagt tttgcagatg cgcctgcgcc
    37741 ccgaggtaaa caggcttatc aggccagtga tgcgtgcagg tgtcgagggt tttactcatc
    37801 agttgctgcc ccactttttc gccacgcaac gcttcactga caatcacccg accaataacg
    37861 accggctcaa gatcatcatc acttttcaga atcctcgcat atgccaccag ttcatcattt
    37921 ttccaaccga gaatatggcg attatccccc gtcaaatcat caccatcgat atcctgataa
    37981 gggcaattct gctcgacaac aaacaccgcg caacgcagtt gtaataaggc ataaagctgg
    38041 gaaaccgata attcagagtg atgcagatct tgccattcaa tcataatgct ctccttttag
    38101 atgtatgcct tatactagcc ggaatatttt ttgaaacgtg atgaactcat ggaattaatt
    38161 tttttaggta cttcagccgg tgtgccaacc cgcacgcgca atgtcacggc aatattgctt
    38221 aacctgcaac atccgaccca gagcggactt tggttgtttg actgcggtga aggcacccag
    38281 catcagctac tgcataccgc ctttaaccct gggaaactgg acaagatttt tatcagtcac
    38341 cttcatggcg atcatctctt tggtttaccc ggcttgctgt gcagtcgttc tatgtcaggc
    38401 attatccaac ccttaacgat ttatggtccg caaggtatcc gtgaatttgt ggaaaccgcg
    38461 ctgcggatta gcggctcatg gaccgattat ccgctggaaa ttgtcgaaat tggcgctggc
    38521 gaaattctcg atgatggcct gcgcaaagtg accgcttatc cgatggaaca cccactggaa
    38581 tgttatggct atcgtattga agaacatgat aaaccgggtg cattaaatgc ccaggcatta
    38641 aaagctgctg gcgtgccgcc tggcccactg tttcaggaat taaaagcggg caaaacaatc
    38701 acgctggaag atggaaggca gattaacggc gcagattact tagctgctcc agtgccaggt
    38761 aaagcgctcg ctattttcgg cgataccggc ccctgcgatg ccgcacttga cctggctaaa
    38821 ggtgtcgatg tcatggtgca cgaagcgacg ctggatataa ccatggaagc caaagccaat
    38881 agtcgcggcc atagctctac acgccaggct gcgacactag cccgtgaggc tggagtcggc
    38941 aagctaatca ttacccacgt cagctcgcgc tatgatgaca aaggttgtca acacctgtta
    39001 cgtgaatgca ggtcaatttt cccggcgact gaactggcga atgatttcac cgtgtttaac
    39061 gtttaa
//
LOCUS       DAESHI010000031        38571 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.031, whole genome shotgun sequence.
ACCESSION   DAESHI010000031 DAESHI010000000
VERSION     DAESHI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38571
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.031"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..843)
                     /gene="adrA"
                     /locus_tag="KW011_001621"
                     /gene_synonym="dgcC"
     CDS             complement(<1..843)
                     /gene="adrA"
                     /locus_tag="KW011_001621"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HBH7659979.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRG"
     gene            complement(945..1265)
                     /gene="psiF"
                     /locus_tag="KW011_001622"
     CDS             complement(945..1265)
                     /gene="psiF"
                     /locus_tag="KW011_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HBH7659980.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(1384..2799)
                     /gene="phoA"
                     /locus_tag="KW011_001623"
     CDS             complement(1384..2799)
                     /gene="phoA"
                     /locus_tag="KW011_001623"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HBH7659981.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2900..3160)
                     /gene="iraP"
                     /locus_tag="KW011_001624"
     CDS             complement(2900..3160)
                     /gene="iraP"
                     /locus_tag="KW011_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HBH7659982.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(3342..3545)
                     /locus_tag="KW011_001625"
     CDS             complement(3342..3545)
                     /locus_tag="KW011_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7659983.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            3623..4717
                     /gene="ddlA"
                     /locus_tag="KW011_001626"
     CDS             3623..4717
                     /gene="ddlA"
                     /locus_tag="KW011_001626"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBH7659984.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(4741..4953)
                     /locus_tag="KW011_001627"
     CDS             complement(4741..4953)
                     /locus_tag="KW011_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HBH7659985.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            5213..5521
                     /locus_tag="KW011_001628"
     CDS             5213..5521
                     /locus_tag="KW011_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HBH7659986.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(5580..6674)
                     /gene="yaiW"
                     /locus_tag="KW011_001629"
     CDS             complement(5580..6674)
                     /gene="yaiW"
                     /locus_tag="KW011_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HBH7659987.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(6687..7907)
                     /gene="sbmA"
                     /locus_tag="KW011_001630"
     CDS             complement(6687..7907)
                     /gene="sbmA"
                     /locus_tag="KW011_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HBH7659988.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            8259..9416
                     /gene="ampH"
                     /locus_tag="KW011_001631"
     CDS             8259..9416
                     /gene="ampH"
                     /locus_tag="KW011_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HBH7659989.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(9417..10040)
                     /gene="iprA"
                     /locus_tag="KW011_001632"
     CDS             complement(9417..10040)
                     /gene="iprA"
                     /locus_tag="KW011_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HBH7659990.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(10128..13016)
                     /locus_tag="KW011_001633"
     CDS             complement(10128..13016)
                     /locus_tag="KW011_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBH7659991.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITVTNGGVLDVNTNMNDISSVF
                     ATDTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMN
                     SDGHLTVNGNNDINNGDEVDNSSADNVVAATGNYKVRIDNSTGAGAIADYAGKQLIYI
                     DDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNL
                     EQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGN
                     NAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDL
                     SATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSND
                     MKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNG
                     TEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            13541..14515
                     /gene="hemB"
                     /locus_tag="KW011_001634"
     CDS             13541..14515
                     /gene="hemB"
                     /locus_tag="KW011_001634"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBH7659992.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(14621..15472)
                     /gene="tauD"
                     /locus_tag="KW011_001635"
     CDS             complement(14621..15472)
                     /gene="tauD"
                     /locus_tag="KW011_001635"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HBH7659993.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(15469..16296)
                     /gene="tauC"
                     /locus_tag="KW011_001636"
     CDS             complement(15469..16296)
                     /gene="tauC"
                     /locus_tag="KW011_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HBH7659994.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(16293..17060)
                     /gene="tauB"
                     /locus_tag="KW011_001637"
     CDS             complement(16293..17060)
                     /gene="tauB"
                     /locus_tag="KW011_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HBH7659995.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(17073..18035)
                     /gene="tauA"
                     /locus_tag="KW011_001638"
     CDS             complement(17073..18035)
                     /gene="tauA"
                     /locus_tag="KW011_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH7659996.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            18645..19316
                     /locus_tag="KW011_001639"
     CDS             18645..19316
                     /locus_tag="KW011_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBH7659997.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            19326..20522
                     /locus_tag="KW011_001640"
     CDS             19326..20522
                     /locus_tag="KW011_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH7659998.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIKRGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            20522..21082
                     /locus_tag="KW011_001641"
     CDS             20522..21082
                     /locus_tag="KW011_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBH7659999.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            21084..21857
                     /locus_tag="KW011_001642"
     CDS             21084..21857
                     /locus_tag="KW011_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HBH7660000.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            22045..22320
                     /gene="frmR"
                     /locus_tag="KW011_001643"
     CDS             22045..22320
                     /gene="frmR"
                     /locus_tag="KW011_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HBH7660001.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            22355..23464
                     /gene="frmA"
                     /locus_tag="KW011_001644"
     CDS             22355..23464
                     /gene="frmA"
                     /locus_tag="KW011_001644"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HBH7660002.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            23557..24390
                     /gene="fghA"
                     /locus_tag="KW011_001645"
     CDS             23557..24390
                     /gene="fghA"
                     /locus_tag="KW011_001645"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HBH7660003.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(24616..25155)
                     /locus_tag="KW011_001646"
     CDS             complement(24616..25155)
                     /locus_tag="KW011_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HBH7660004.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(25257..26467)
                     /gene="mhpT"
                     /locus_tag="KW011_001647"
                     /pseudo
     CDS             complement(25257..26467)
                     /gene="mhpT"
                     /locus_tag="KW011_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
     gene            complement(26754..27767)
                     /gene="dmpG"
                     /locus_tag="KW011_001648"
     CDS             complement(26754..27767)
                     /gene="dmpG"
                     /locus_tag="KW011_001648"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HBH7660005.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(27764..28714)
                     /gene="mhpF"
                     /locus_tag="KW011_001649"
     CDS             complement(27764..28714)
                     /gene="mhpF"
                     /locus_tag="KW011_001649"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HBH7660006.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(28711..29520)
                     /gene="mhpD"
                     /locus_tag="KW011_001650"
     CDS             complement(28711..29520)
                     /gene="mhpD"
                     /locus_tag="KW011_001650"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HBH7660007.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(29530..30396)
                     /gene="mhpC"
                     /locus_tag="KW011_001651"
     CDS             complement(29530..30396)
                     /gene="mhpC"
                     /locus_tag="KW011_001651"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HBH7660008.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(30414..31358)
                     /gene="mhpB"
                     /locus_tag="KW011_001652"
     CDS             complement(30414..31358)
                     /gene="mhpB"
                     /locus_tag="KW011_001652"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HBH7660009.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(31360..33024)
                     /locus_tag="KW011_001653"
     CDS             complement(31360..33024)
                     /locus_tag="KW011_001653"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HBH7660010.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLHVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            33066..33899
                     /gene="mhpR"
                     /locus_tag="KW011_001654"
                     /pseudo
     CDS             33066..33899
                     /gene="mhpR"
                     /locus_tag="KW011_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
     gene            33976..35058
                     /gene="lacI"
                     /locus_tag="KW011_001655"
     CDS             33976..35058
                     /gene="lacI"
                     /locus_tag="KW011_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HBH7660011.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            35181..38255
                     /gene="lacZ"
                     /locus_tag="KW011_001656"
     CDS             35181..38255
                     /gene="lacZ"
                     /locus_tag="KW011_001656"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HBH7660012.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNHLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTDDGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRFGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGAEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGSYHYQLVWCQK"
     gene            38307..>38571
                     /locus_tag="KW011_001657"
     CDS             38307..>38571
                     /locus_tag="KW011_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH7660013.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFA"
BASE COUNT         9377 a        10291 c         9634 g         9269 t
ORIGIN      
        1 accgcgcagg gtaatttgta actgtcgggt aagcgccaca atcgcttcat cgcccacatc
       61 atgaccccag gtatcgttga tgctcttgaa atggtcgata tcgataatca gtaacgttgc
      121 atcgcgatta tgccgccgac agttatcaaa ttcattgcgt aacatagttt cccaatgacg
      181 tcggttatac acgcccgtca tgccgtcgcg agtactcatg acctgcaacc tgcgtttatg
      241 ttccgccagt ttggttgccg tctggtagct gacccagcca aacagcagag gataaacgac
      301 aataatggga agggagagcc accattccag cggcgcacta ttgaacgaca cggtaatgcc
      361 cgtaagctcg agggtgacaa ggcaggaaac caccatcaac accagacccg cgacaaacag
      421 acgggggccg cctgccccca tcaaattcag acacataatc atcaacatag cggtggaagg
      481 cagcacgttt acgcccatta cgcctaccca cattcccgct aatactgcat cggtttttaa
      541 gttgtaaatt tcccggctaa gcggatcgac ggccctgctc gctatctgcc aggctaaatg
      601 cggccagacg aacgcccagc cgaccaacac cagccaccac cagcccggcg gcgggtgcga
      661 aaccagcgtt gaagcaatcg gtaagaacat gccagccagg ccaaccgcac ggggtagtct
      721 gacgcgacgg gcgaagcgca gcccggaccg ctgatgttcg ttttgaggag ttaaaggagg
      781 ttcctcccca tgcgccgccg cttttttgaa aaagttttca tcattcatta tttttgggaa
      841 cattcagaaa caattttccc aaattataga gacggggcta aaggcagaag tatgaaattt
      901 cgggtgagcc actttcgtag ctcacccgtt agcactatca ggtatcaggc ggctttcttg
      961 aggcaggcac tcataaactt attacgatca tcacctttca gagattgttg cgttgcttga
     1021 ttattacatt cgcgcatctt ttgctgctgt ggcgtcaaac ttttttcacc aggcgcagac
     1081 ttgctgttct tcaggcaatc actcatgtag gtcttacgag catccccttt caacgcctgc
     1141 gccgtcgcct gctgattaca ggaggtcata cgctgttgtt gtggggttaa ggttctctcg
     1201 gcagcgccga cggtggttaa aaaaaccaga ccaaaaagca aggtaaccag taatgttatt
     1261 ttcatagcac catccctctt catgttttaa ccatgagcgt ttgcgcccgt gatctgccat
     1321 taagtctggt tgctaacagc aaaaaaacca cccggcagcg aaaattcact gccgggcgcg
     1381 gttttatttc agccccagag cggctttcat ggtgtagaag agatcggtct ggtcggtcag
     1441 tccaacaaca ttggcggcat gcggaccata cgccgcaata cgcaactgac tgccggtatg
     1501 ttcttgtgaa tcctcttcgg aattcccgta actcatcacc atcactgcgc catctttagt
     1561 atttagcgcc tgagtgaggc ccggcgcttt ggtatccggc gcaacaacct ggctggcgtg
     1621 ggcgtgatca gcggtgacta tgaccagcgt gttaccctcc tttttagcga attccagcgc
     1681 ccgttgtacg gcttcatcga gatcgaccgt ctcgccaatt tgcccacaag gattcgcagc
     1741 gtgatcctgt ttatcgattg acgcaccttc aacttgcagg aaaaatcctt tctcattttt
     1801 actcaacaat tcaatggttt tgtcggtcat ctgtgccagg gttggtacac tgtcattacg
     1861 ttgcggatta ggcgtacagg tgactgcggg cttatcgata ttgccgtggt acgttgcttt
     1921 cggccctagc cagcgcactg gcatattgcc gtcagcaaac aggccaagca ggggtttttg
     1981 ctgattcgct tccgtcaccg aattcagtga ggcagcatcg ttcaccaact gataaccacg
     2041 cgcctgtgcc tgttcacgca gcgtttttcc ctgccattca ccagcggttg ccgtttcagc
     2101 aaaggttttt gcgccgccgc caagcgtaac gtcggcacga gcgttaagca gctgttcggt
     2161 aatcgatcct tttccgcctt tttccagagc gttacccgga catttttcac tggtcgcgct
     2221 cggaccgtag catttgcgcg aagtcacatg tgccaccagc gcagcgggcg tggcatcctg
     2281 caactctgcg gtagaaacgt taccggtcgc cagacctgcg gcttttgcca tttccagaat
     2341 cgttgggtga tctttttcgt gaatatcgac gcccagcgcg ccgttatagg ttttgacacc
     2401 ggttgaccag gcggttgctg atgcagccga gtcggtgacg tagtccggtt tgccagtttt
     2461 tttattcagc gcatagtgag tgtattgccc ggtaagcggt aaggcatcga tacctttaaa
     2521 aaagccgccc gcaccttcgg cataattacg tgcggcagta atttccgaat cccccatccc
     2581 atcgccaatc agcaaaataa tattttttgc aggtttatcg ctaagagaat cacgcagagc
     2641 ggcggtctga tcactcgtta agcggcgagc accgccgggt gcagtaatat cgccctgagc
     2701 agcccggttt tccagaacag gcatttctgg tgtccgggct tttgtcacag gggtaaacag
     2761 taacggtaag agtgccagtg caatagtgct ttgtttcact ttattttctc catgtacaaa
     2821 tacattaaaa aataaaaaca aagcgactat aagtctcggc cgtgacaact ttatgacagc
     2881 tgttgaaaag attaactttt tactgacgag gatgcttcaa taacttcttt acgtaatcgc
     2941 gcagcagctc cgtatcgtcg tcaggaatgc tggcatcggg ctttacctcg tacagcgccc
     3001 cctctacctg atcaatcaac cgctgttggt cattttgcgc catattgcga agcattgcag
     3061 tgacgataat ctccaaagct tctacctgcg cacacagttc tttcgactct tcttcttttt
     3121 gggcaagctt aaataacaac tcagcaatga gatttttcat gtctgtattt ccttatccaa
     3181 agtatggaga agttaacatt atgtttcaca acttcatagt gtctggaagt attattttta
     3241 tgacaaagga aatactttgc gcaaatattt tttgtttgat taccagcgaa acgtttctct
     3301 tatttaaata attacccaca caaaatatag gcttttaatt atcaaccttt attataaata
     3361 aaagttatga aatatattct cttcgttaac ttataattaa gagaaaaaaa ctcaccgcat
     3421 tgccatttaa gccaggaata aattagcttc ccgttcatca tttttgtgat ctcgcgcaca
     3481 tctttgagat aacgcacttt cttcaccagt acgttgcacg ctttgtcagc agaagattgc
     3541 aacaatcttt cagaaggccg cgaatccccc gttttgtcgt ggtaatgtat gcactttgtg
     3601 ttagacggga taggttttta agatggaaaa actgcgggta ggaatcgttt ttggtggtaa
     3661 atcagcggaa catgaagtgt ctctgcaatc ggcaaaaaac attgtcgatg ccattgataa
     3721 aagtcgcttc gacgttgtgc tgctgggcat tgataaacaa gggcaatggc acgtcagcga
     3781 tgccagcaat tatctgctaa atgcagacga tcctgcccat attgcgttgc gcccttcggc
     3841 gaccagcctt gcgcaggtgc caggtaaaca tgagcatcag cttatcgacg cgcaaaacgg
     3901 tcagccgttg ccgacggtgg atgtcatttt cccgattgtc cacggtacgc tgggcgaaga
     3961 tggttccttg cagggaatgc tgcgggtcgc caatttaccg tttgtaggtt ctgatgttct
     4021 ggcttcagca gcctgtatgg ataaagatgt caccaaacgt ctgctacgcg atgccgggct
     4081 gaacattgcg ccatttatta ccctgacgcg cgctaatcgt cacaacatca gttttgccga
     4141 agtggagtct aaactggggt taccactgtt tgtaaaaccg gctaatcagg gctcttctgt
     4201 tggtgtcagc aaagtaacca gtgaagaaca gtacgcaatt gccgtcgatc tggcgttcga
     4261 gttcgatcat aaagtgatcg ttgagcaagg gatcaaaggt cgtgagatcg aatgcgcagt
     4321 tctgggcaac gacaatccgc aagccagcac ctgtggcgag atcgtactca ccagcgattt
     4381 ctatgcctac gacaccaagt acattgacga agatggcgcg aaagtggtag ttccggcagc
     4441 cattgcacca gagatcaacg ataagatccg ggcgattgcc gttcaggctt atcagacgtt
     4501 gggatgcgca ggcatggcgc gtgtagacgt gtttttaacc ccagagaacg aagtggtgat
     4561 caacgagatc aacacactgc ctggcttcac caacatcagt atgtatccga agctgtggca
     4621 agccagcggt ctgggttaca ccgatctgat cacacgtttg attgagctgg cgctggagcg
     4681 tcacgctgcg gataacgcat tgaaaaccac aatgtaatat tttaacgccc gataatattc
     4741 ttattgggcg ttttcagttt cttcatcgcg acgacgaata atcaaccccg ccagccagaa
     4801 actaatcacc cacgtcacca gaccgacggc ataggtctgc caaccttttg cttcaaatcc
     4861 cagtaacccc accacgccat taagaatgaa tataaggcca atggcgaacg catagtagtg
     4921 ccagtcacgg cggattttta caggcagatt catggcagct ccgttaacga aaaacaacat
     4981 ccttgcatat tctgtaacaa ccgtctgtgg tgtagacaac attttcgtaa ttgttaataa
     5041 atttaactta aggttgccat tttgtaactc acaacgaaga ttcacgcttc gggaatattc
     5101 ccaacatcaa tttaccatga ttctgatatt gacaatctaa atgacctgtc agacttgcca
     5161 taagccgctg gtatcacacc agaagagaag cagatgactt ccggaggttt ttatggctga
     5221 tttcaccctg tcaaaatcgc tgtttagcgg aaaatatcgc aatgcctctt caacgcctgg
     5281 caacattgcc tatgcgttgt ttgtgctgtt ttgcttttgg gctggggcgc aattgctgaa
     5341 cctgttagtg catgcgcccg gcgtctatga gcgtttaatg caggtccagg aaacaggtcg
     5401 cccacgggtg gaaattggtt taggtgtcgg caccattttc gggctgatcc cgtttttagt
     5461 aggctgcctc atttttgcag tggtggcgct atggctgcac tggcgacatc gccgccagta
     5521 agatttacgt tgccagatac ggcctaaaac gccttatctg gcatgacatt cctgtttgat
     5581 cactgtttca tacaacgcgc ccgccgttcg tctacgcgct tcgcaaacca ggccgtagtc
     5641 aggttgcggg tgattttcgg gctttccagt tgaatgccag gcaacatttc gcggggtagt
     5701 gatttacccg ttttcgcttc ggcaagttga taaactttct tgtacagcgc cgtctcctca
     5761 aaagagaagc tgtcaccttt ctctaactga cggcgaattt cgctgtcgct catccccagt
     5821 tttcccgcca gtttgcgcgt tgccagttcg gttttcccgg gttctttact gtcgtaacga
     5881 attaaatcac cgtccagtgc cagcttcacg ccgctggcct tactgactgc gttctgaaac
     5941 gcggcattac ggctggcgta ccagccagcg ttaaaatcag caaagcggta tatcggtgcg
     6001 ctatagctgg cgggataatt cagtaaatgg taagtaccaa accacaaccc gccgcggcga
     6061 ctgaagactt cctgacgaac tgtaccgtcc attttccacg gatacccttt ggtatgctgt
     6121 tcggcaaaag caatgctgac ctgcatcggc ccaccggtgc gcaccggatt aagcgaacca
     6181 aacagcgtct gccccattgg taccatgctg atcagatcgt caaaaatcgc gctcaattgc
     6241 ttttctgtac gcaccgaatc taaccgttcg ctataacttt tgccgtttgg cgatttgatt
     6301 tttagcgcgg tatgaaccag aaaagcagga atgtgcatcc gttcagcacg acggtcaatc
     6361 tcttgccagg cgatcttgct taagcccgga acggccggat ctgcctgata atttgactcc
     6421 tgctgcgcca ccgccagcac cgagcagacg ttttccagcg tcggtgccag cccctgactt
     6481 tcaaaggtga tggcgagatc tttcgcccag gcatcgcggt ctttcacgct cgcaggcatc
     6541 ttctgccgca cgacgctcgc tacatctatc gctttttcgc cctttttcaa cggttgcgga
     6601 gcctgactac tacaccccgc taaaaccaga accgccaaca atgacagcga agatagatga
     6661 ttgacgcgcg acattacttc tcctttttag ctcaaggtat gggttacttc ctgaattttg
     6721 tcaccatcca gctcatgttc aaagctgcgc agacgtttgt agatagacat caactcaacc
     6781 agtgtggtcc atgagttaat caggtactgg aaagcaccgc gaacctgacc aaaaacgttg
     6841 gtaatctgcg tcatcaggcc gagcgtaatc gtaccggcaa caattgacgg aaacagcaag
     6901 aacaaaccga aaacgttatc gacctgcaaa tagagaatgc gggcgatgtt gaaatacata
     6961 tagtgaaaat agaggcgaaa atagtttttc cgtacggcgc taaacagctc gcgtaccgta
     7021 ggcggcgtcg cgcgcgtggc atcgtcttca ccataaacca gctctttacg gtaggcggcc
     7081 tctactcgct ggtttttaaa ctccagcccc ggcagtttga tccctaccac tgccagccat
     7141 ccggtcccca tcagcgacca gacgatagcg gcaatcacca gaccatacgg aatgtgcccg
     7201 ataatcggca gctccggcac atgcgcggag agcgttacca gcaccggcag gaaggcgatc
     7261 aacgtcatga tggcgttgat aaaactgacg cccatattct ccagcgttga agcaaaacgc
     7321 atggtgtctt cctgcacacg ctgtgcggca ccttcgatat gacgcagttg ttgccagttc
     7381 gccatgtaat attcgttcat cgccgtacgc cagcggaaca catagtgact gacaaagaag
     7441 ttgttcagta cactgatcac caccgcgatc agcgcaatcc ccagaaagac gcccacttcg
     7501 cggtaaaatt gttcgatagt gactttatgc ggcgaactta gcgcggtttg aatcaaatca
     7561 tagaacggtg cataccaggc gttgacggcg accccgactt ccaccaaaaa ccaggtgacg
     7621 aagatgatca gtgcagtacc gagtattgac cagtattgcc aacggtgcgg gctgtagata
     7681 aaccagaaaa atgcaaaaag tcctacgcaa acaatgtagt aagcgtaaaa aatcaggaaa
     7741 tccaacgacc agaaacgcgc ggcgctaatc gggatctgcc cggaagcgcc ggtgatacgc
     7801 gccacccagt ctcccccacc ggcttgccag aagataacgg caatcaatgc ccaaacaaag
     7861 gccgagagaa aaaacgctcc cggctttggg aaaaaagact taaacatatg cactcctgct
     7921 aacttcttat cgttttaaca actgctgtgt attaaccgca tgaccactac aaagcgtcag
     7981 ccaaatgaaa ccgcccattg tgacccatag caaggcgctt agttcgccag cctgaccata
     8041 agaattgttt cgacggattt cacaaaccaa agattgttta cataagcgga ttaacacgcg
     8101 caggatcaca aatgcaggca ttggtgagca ggaaaaagat cgtgtttacg cgccgttacg
     8161 tttttaacac tattattgca ctaattcgcc ctttgcaatc catcaatgag tagtataaat
     8221 acgctcagtt accttcattc aatctatgga caccaccgtt gaaacgtagt ctgctttttt
     8281 ctgccgtgct gtgtgcggcg tcattgacct ccgtccatgc ggcgcagccg atcactgaac
     8341 cggagtttgc ctctgatatt gtcgatcgtt atgccgatca tattttttac ggcagcggtg
     8401 caacggggat ggcgctggtt gttatcgacg gtaaccagcg cgtctttcgt agttatggcg
     8461 aaacgcgacc tggtaataac gttcgcccgc agctggattc ggtcgtgcgt attgcttccc
     8521 tcaccaaact aatgaccagt gaaatgctgg tgaaattgct cgaccagggg accgtaaagt
     8581 taaacgatcc gctaagtaaa tatgctccgc caggcgcacg cgtgccgacc tacaatggga
     8641 cgcccattac cctagtgaat ctggcaaccc ataccagcgc cctgccccgt gaacagcccg
     8701 gtggcgcggc acatcgtccg gtgtttgtct ggccaacgcg cgagcaacgc tggaaatacc
     8761 tttctacggc gaagctgaaa gccgcgccag gcagtcaggc ggcgtactct aaccttgcgt
     8821 tcgacctgct ggcagatgca ctggcgaatg cgtccggcaa gccttatacc cagttgtttg
     8881 aagagcagat tacccgtcca ttggggatga aagacaccac ctacaccccc tcaccggatc
     8941 agtgccgccg tttaatggtt gctgagcgcg gtgccagtcc gtgcaataac acgctggcgg
     9001 caattggcag cggcggcgtc tattccacgc ctggcgatat gatgcgctgg atgcagcagt
     9061 atttgtcatc tgatttttat cagcgcagta accaggcaga tcgcatgcag acgttgattt
     9121 atcagcgcgc gcagtttacg aaagtgattg gcatggatgt ccccggcaaa gcggatgcgc
     9181 ttggtttagg ctgggtatat atggccccga aagagggtcg tccggggatt attcagaaga
     9241 caggcggtgg tggcggtttc attacctata tggcgatgat cccgcagaag aatattggcg
     9301 cgtttgtggt ggtcactcgt tcgccgttga cgcgctttaa aaatatgagc gatggcatca
     9361 atgatctggt gaccgagcta agcgggaata aaccgttggt tatccccgca tcctgattaa
     9421 tactctgaag gcaaacggtt gatagcgacc agtttgcctt tattcatttc gatatagccg
     9481 cctttacgca aagcagcgag aacttctgcg acgacagaac gcgatatgcg tgtacgttga
     9541 tggatataat tcatcacacc aatacgtgat cgcaattctt cattccagtc aatcattgat
     9601 aataatgttg cgcggatttg ttcgtaggaa ttatgcccaa tgagctgcac gtcgcgtaat
     9661 tccagaattc tattttgcca ggctaaccag taaaaagcgt ctcgccagag ttgattttgt
     9721 tcaataagcg taatggtttg tttggctggt agatgatatc ccgtacaatt tccttctgat
     9781 attaatttgt atggtatatc gtttttcatt aaaccatcag ccagccccat aatataaggg
     9841 gcctgggtaa taccgataag tacgttttct tctctacgca gagagataac tccctctaaa
     9901 ataacgaaag tatcttcgtt attgacatca ctggaaaata taacttgctt ttcattatta
     9961 aactcgaagc gcgtaccgta tctggacaaa catttatcga gcttaccaaa ttcctgaaga
    10021 ggtttaacta cagataacat ttgcgcgtcc tttgcagtaa tgcccgtcaa atccttgacg
    10081 ggcattattt agattaaatt accagtattt cttcggagtg aagaatatta ccaggtatat
    10141 ttaacaccca cgttcgcgga ccagtcttga tctacgtcac caccaccgag gtagttagca
    10201 tcggtatagg cgctgaagtt cttggtgaag ctgaactgag tacccagacc aacacgtacc
    10261 gcagaccctt cagtaccgtt atcgatggaa tcaccgttca catcgttatc gttgttagag
    10321 tcgtcgtaga cgtaagccag tttgaagtac ggagtcagag cctgatcttc gctgtaggtg
    10381 aaggtataac ctgcatctac acccagttca taacgcatgc tgtcgtaaga ctgaccgtca
    10441 actttcatgt cgttgctcag ctggtagtca tcaccagact ggaacagacc agaaacgctg
    10501 ccgtaaggag tcacgtaacc agcatcaccc agtttgaagt cgtaaccggc tttcaagccg
    10561 aagccccaag cgtcggagtt ggtgctaccg tcaacgtaag taccgttgct catggttgca
    10621 gacaggtcgt tgttgaagtg agagtagctc aagctaccat caacaaagac gttgttcgcg
    10681 aagtgagcag aagagtagat gtaggcagtc tggctgtctt gatctacctg accagaacgg
    10741 tcattcatgt cacctttagc gaagcctgca gccgcaccga cgatccactt agcgttgtta
    10801 ccgtcaactt tggtatcaac accgaccatg atgccgttaa catcctgatc atagttgatg
    10861 gtgccgttgt cgccgttgaa gttaccaccg aagtagctta cccatgcgcc gccgttatca
    10921 gccaggccat gacgagagtt ggtcagacga gtaccaacgg tgtcttgttc caggttccag
    10981 atattggtgt tcgcagacgg gatgctcagc gccatgttag cgtagtcggt cagctccatc
    11041 tgttgcagaa caacggtgtt accgcgctgt tcagcctgat aggtgtatgc acccaggtca
    11101 gctttgttag cagcagagaa ggtcgcgttg gttttggtat catcgatata gatgagctgc
    11161 ttacctgcgt aatcagcaat cgcaccagcg ccagtggagt tgtcgatacg aactttgtag
    11221 ttaccggttg cagctacaac gttgtctgca gagctgttgt caacttcgtc gccattgtta
    11281 atgtcgttgt taccattaac ggtcaagtga ccatcagagt tcatcgcaat aacaccataa
    11341 ccgtagttag ctttgctggt gtcgttggta cgatcgttga cgaggtctgc attcaggatg
    11401 taatcattac tatgaatgtc aaacacacct gcgtaaacat taccgttgtt atctttaacg
    11461 ttgccactgg tcagttccag agtatcagtt gcaaatacgc tggagatgtc attcatgttg
    11521 gtgtttacat ccagcacacc accgttagta acggtaatgg tattggcata cagcggtgca
    11581 tatgcccaag ctgaagcagt atcttcaccg aagttaacgg ttgagaaggt atcaatggtc
    11641 aggtgatcgg tgttaacttc accgttaact tccattgaag aaccaccgtt cagagtgatg
    11701 gtgtcagaag tcagagaaga atctgcactc acgttaacct gagaaccgct gttgatcgcc
    11761 agggtatcga tcagggaagt tttcgtggtt tcccaatcag aaccgttgtt cagaacaaca
    11821 tcgaatacgc cgctctggaa tgcgttagcg ttatcaacac gattccagcc atcttcagag
    11881 taagaagcgt acgggttcag gctgtttggt gcaacgtcgt agaaggcttc gtcagcaaca
    11941 acaacgtcat aatcaatatt cgctgaacca acccatttgg tgttgttcag agtcaggttc
    12001 aggtgatcga cgttctgatc gtcagttgcc caaccgccat gagtgtaagc agtggtagag
    12061 ttgtcaatca ggtgacccgt cgcgtcaaag ttagcgttcc aggtactgtc aaatacaacg
    12121 tcaccaagca actgagagtt gttgaacgta acgttgttga ccatacgata atcagaagtg
    12181 tcatcatcga tgaaggctaa cgcaacatca tcagcaggag atgcgccatt gccgtagtca
    12241 cttggttcaa cagaattgcc gaaatgacca cgatctgatt gttcgtcttc agtccatgaa
    12301 ccagaagtta cagtagagtc attaacaacg atgttgttgt cccactgatg accttcggta
    12361 ttgtaggtcg cattaccgag gtcgtgttga accaaagtaa tacctgcaac atgggagttg
    12421 ttgctgatgt tgatgttact ttcaacatcc agagtcacag cggtaccaag accagcggtt
    12481 accaaacgat cataatctgt cttagatgtt ttaccgtctg catccagata agtatcggtg
    12541 aagtagaagg cgtcaaaatc atcgtcaata gtagaattag cgatgttcag agtgaagatg
    12601 tcgccatcaa cccagtcgtg gccagtataa tcgctaccgt tggtccagac gtagtcgcca
    12661 taaccaacct ggtaagatgt aaccatacca tgaacaattg agttggagat gttcagtgta
    12721 ttaccggtat ctgtgccata atcgtggttc agataataag tagaaattac accatttaca
    12781 gtgtcgccgc tgaaagtggt atcaaagtaa ccatcagctg cgtggtcata tacataacca
    12841 ccgtaagtca gataatcgtg atcaatttta gtgctgtcac cccaattgta gatggtggct
    12901 gcgtcatcgt gatacccgaa ggtgttataa gtagctgcat tagcctgaga ggtgatagcc
    12961 agagtgcaag ccaatgctaa ttgtgatact acaagtttct ttttccagga gtgcatttgt
    13021 catccctcct cagggacgta ataagttgat ccatcaatta tcaatgcata gaaaacatat
    13081 tatgtttcgc gctaacgatt atgctgcgtt acagacgaat ggttcaatta ttctttcccc
    13141 taagtccgaa tgtggacata tataatttat ggagaaaaac acgcatcaaa ttgataaaag
    13201 gacgatattt caacatgttg cagataatgt tatttttgat acatacgcgt tataaatatc
    13261 aaaaacacaa accagataaa tatcttatca atatacaaca aagtttgaat tattcaattt
    13321 atagcagcac aaccaaacgc caaaaaaata ataaccaaga gctataaata aagattaaaa
    13381 atattttcag tgaatatgaa attattgcta tttctattac aaagatgtcc tggtctctct
    13441 ttcattctct tcttgtcaac atcgcgacaa ctttcgtaaa acactcctgc cccgcttcag
    13501 gtatactgtg caccttaatt ccacaaacat caggcagacc atgacagact taatccaacg
    13561 ccctcgtcgc ctacgcaaat ctcctgcgct gcgcgctatg tttgaagaga caacacttag
    13621 ccttaacgac ctggtgttgc cgatctttgt tgaagaagaa attgacgact acaaagccgt
    13681 tgaagccatg ccaggcgtga tgcgcattcc agagaaacat ctggcacgcg aaattgaacg
    13741 catcgccaac gccggtatcc gttccgtaat gacttttggc atctctcacc ataccgatga
    13801 aaccggcagc gacgcctggc gggaagatgg actggtggcg cgtatgtcgc gcatctgcaa
    13861 gcagactgtg ccagaaatga tcgtcatgtc agacacttgt ttctgtgaat acacttctca
    13921 cggtcactgc ggtgtgctgt gcgagcatgg cgtcgacaac gacgcgactc tggaaaattt
    13981 aggcaagcaa gccgtggttg cagctgctgc aggcgcagac ttcatcgccc cttccgccgc
    14041 gatggacggc caggtacagg cgattcgtca ggcgctggac gctgcaggct ttaaagatac
    14101 ggcgattatg tcgtattcga ccaagttcgc ctcttccttt tatggcccgt tccgtgaagc
    14161 tgccggaagc gcattaaaag gcgaccgcaa aagctatcag atgaacccaa tgaaccgtcg
    14221 cgaggcgatt cgtgaatcac tgctggatga agcccagggc gcagactgcc tgatggttaa
    14281 acctgctgga gcgtacctcg acatcgtgcg tgagctgcgt gaacgtactg aattgccgat
    14341 tggcgcgtat caggtgagcg gtgagtacgc gatgattaag ttcgccgcgc tggcgggtgc
    14401 tatagatgaa gagaaagtcg tgctcgaaag cttaggttca attaagcgtg cgggtgcgga
    14461 tctgattttc agctactttg cgctggattt ggctgagaag aagattctgc gttaattttt
    14521 ttcctctcag aaagggggag ttaacctgtt gaaagtacgg tgaacgcccc ctcaccctaa
    14581 ccctctcccc aagggggcga gggaaccgtc cgcactcgta tcaccccgcc cgataaaacg
    14641 gtttatcccc aaggatcgtc gcccgatgca taatccgccg ctgtggcagg taatcggcat
    14701 tggcatagtg ctgagtcacg cggttatccc aaatcgcaat atcattcggt tgccagcgcc
    14761 aacgcacctg aaactccggt ttagtgatat gggcgaataa aaagcctaac aaggcttcgc
    14821 tctctttctc gctgacatcg acaattcgcg tagtaaagcc ttcattcaca aacagcgcct
    14881 gtttaccgct caccggatgc gttcgcacca ccggatgtag caacggagga tttttcgcga
    14941 ccgcctcgcg ccagcgttga tgttcctcct cggttttgcg gtatttgtat tccgggaacg
    15001 atttacggaa atcatgctcc gcacgcagcc cgctcagcag ctggcggaag ggaacagaga
    15061 gcgcctcata ggccgcaata ccgctggtcc agagcgtatc accgccggtc gaaggtaact
    15121 ctttagctgc cagaatcgcc cctgcgggtg gcgtttcaat aaatgtcaca tcggtatgcc
    15181 agttgtcgtt atccggcgga ttatcgttat gggtatccag cacgatgatc tcgtcaaccc
    15241 cttcggcatg agggtaaaca gggtgaatat gcagttcgcc aaaacgctgg gccagcgcgc
    15301 gttgctgctg cggcgtaata gcttgatcgc gcagaaatac cacctgatag cgcagcaccg
    15361 catggtaaag ctgttcaaac tgattatcgc ttaacgggcg cgtcaggtcg gcacccgaaa
    15421 tttgtgcgcc gatatacggc cccagcgggg taatactcag acgttcactc attgtacttc
    15481 tccatgccag ggcgtcagac ggcgctgtaa cgcgcgcaga cccagttcta aaagaaaggc
    15541 gataatcgca atcaccgcga tccccgccag taccacgtca gtcgcgagaa attcacccgc
    15601 tgactgaacc ataaatccta aaccgcgtgt cgcggcaatc agctccgccg ctaccagcgt
    15661 agaccagccc acacccagac caatgcgtaa tccggtgagg atttccggca gcgcgccggg
    15721 caaaatgaca aaccacagca cctgcgcacg gctggcaccc agcgactggg cggcacgaat
    15781 gcgaacctgc tgcgcgcttt tcacccccgc cagcaccgac atcgccaccg gtgcaaaaat
    15841 cgctaaatag atcaataaga tcttcgaggt ttcaccaata ccaaaccaga tcaccatcag
    15901 cggcaaataa gccagcggcg gcaccggacg ataaagttcg attaccggat ccagaatgcc
    15961 gcgcaccgtc gggcttagcc ccatggcgat cccgaccgga ataccgatca ccaccgccgc
    16021 gagtagcgcc agcacaatgc gcgtcaggct ggctgccaga tgctgccaca gcgtggcgtc
    16081 cataaatccc tgcggtccgg caatggtgag cagttttgcc agtacttgtt gcggcggcgg
    16141 caaaaacagc gggctaatca gttgcagcgc cgccaccgtc caccatacag tgagtaaaac
    16201 cgctaacgtg ccaatgctta aggtcacctg acgcgagagc ggccagcgcc atttcagccg
    16261 ccgcgaatgc agtttttcat taatgagcac actcatgaga acgcctcccg ttgctcaaat
    16321 acgcggctta aaacatattc gcgcatggcg atgaattgtg gatcggactt gatgctgcgg
    16381 ctcgactctc ccgcaacaaa gcggcgagca aagttgagcg gcagccgctc cagcacacgg
    16441 ccagggccgg atgaaagcag aaccagttca gtcgccataa acaccgcttc ttctatatcg
    16501 tgggtaatca acagcacctg cttgcccgtc tcctgccaga gtttcagcag cagagtttgc
    16561 atctggtcgc gggtgaaggc gtccagcgca ccaaacggtt cgtcgagtaa taacagctgg
    16621 ggattcgccg ccagcgcgcg ggcaatcccc acccgctgac gttgaccacc ggaaagctgc
    16681 cagatatagc gtttttctgc gccttccagc cccacttttt tcagcatctg gtgcgcgatt
    16741 tccagtcgct gcattttctc tatacctgcc aattgcaggc cgaacgccac gttgtcctgt
    16801 acattgcgcc acggtagtag cccttcattc tgaaaaacca ccccgcgctc tgctcccggt
    16861 ccctcaatac gcttacccgc cagttgaatg ctgccatgct gataaggcac aaaaccggca
    16921 atcagattca gcagggtggt tttaccgcag ccggacggcc ccagcaccac cagtagctcg
    16981 ccgctttcca gcgtcaggtt gatatcttcc agtgccggtt tgccgccata atcggcatag
    17041 agatgagaga tttgcagcat ctgcgcctcc ttttattgca cgaagcgcga ggtaacgtac
    17101 tggctgtaat cattcgctac agccgggacc ttgccctgct ctttcaaaaa ctgcgcggtg
    17161 tcgatgattg ctttgttcac cggtccggtc agttctgccg tttgttgctg cggcgtcaga
    17221 taggtattcc ccttcaccag ccccggaacg tcaccttccg gcacgccgct taaacgcgcc
    17281 agtttgctga tgttttccgg ctgtttcagc cacgcgtctg ggttagcaat gtacggttgc
    17341 tgagcatcga tggcgctttt agcgaacgct ttcacgacct caggatgttt ctcggcaaaa
    17401 tctttgcgca ccacccagac gtccagcgtt ggcgcacccc actgcccgac ctgttcagaa
    17461 tcggtcaaca ccttgccgtc tttttccagg gcgttaaccg ccggtgccca gacataagca
    17521 ccatcaatat caccccgctg ccatgcggcg ataatcgcgg gcggctgcag gttcacaatc
    17581 tccacttgcc ctggtttaat cccccagtgt ttcagcgccg ccagcaggct gtagtgggtg
    17641 gtggagataa acggtacggc aatgcgtttg ccaatcaaat cttcgggttt gctgatagtt
    17701 ttctttacca ccagcgcttc ggagttaccc agttttgacg ccagcaagaa gacttcaatc
    17761 ggcacctgtt ggctggctgc aaccgctaat gggctggaac cgaggttgcc gatttgcacg
    17821 tcgccagaag ccagcgcccg cacgatgctg gctccgctgt caaacttacg ccagtccacg
    17881 ctcgccccgc tctctttagc aaaggtgttg tcggcctgag ccactttcgc cggttcggct
    17941 gaggtttgat acgccacggt gacgttcacc gcctgtgcct gaaaagcgat gaatgccagt
    18001 gcggcaagaa gtgtgttacg cgatgaaatt gccatgattg tctgctcccc tgtcttgtta
    18061 tgggagcagt attcaggaat aaaaacattc attaaaagaa ttagtcgtta tcgcacagat
    18121 gattttattc ttagcaaaaa aacggcaaag gaaagattaa atatgcaatt actccgttct
    18181 aacaattggg aaaattacta cacttcattt tagtgatgca taaacaacca cattacgaga
    18241 tttattactt cattgtttct atcaatacat ataataaaac tattatattc gcattgtata
    18301 tatcactgta tcattctcaa tcactcgcat attacattca cataatgtat ataaacaata
    18361 cttgttcaca gaacatattt tttaaatgca attaattgac aaactaaatc acatctaaat
    18421 acaatgtata aaacaaaata actatacata ccaaacatac aaacacttaa catatgaatt
    18481 gcataaaaac attacccagt cgaacctccg ggtctgaaag cccttgcttt tcagttatta
    18541 gcaacattta acatttataa attaacatct ggctgcgcca gagattacgt ctctgagtgc
    18601 atcgttgtgc ttgtagtata ataaataaaa tggagtgtca ttaagtggat aaggttttag
    18661 attcagccct cctttcttcg gcaaataaaa gaaagggtat ccttgctatt ggcgcacatc
    18721 ctgacgatat agaattaggc tgtggcgcat cgcttgctcg tcttgcgcaa aaaggaattt
    18781 atatcgcagc agtggtgatg actaccggca actctggtac agatggaata atcgatcgcc
    18841 atgaagaatc gcgcaacgcc ctaaagatat tagggtgcca ccaaactatt catcttaatt
    18901 ttgctgacac ccgcgctcat ttacagctca atgatatgat ttccgccctg gaagacatca
    18961 ttaaaaatca aattccttct gatgttgaaa tcatgcgggt atataccatg catgatgccg
    19021 accgccatca ggatcatctc gctgtttatc aagcttcaat ggttgcctgc cgcactattc
    19081 cacaaattct cggctacgaa accccaagta cctggctttc atttatgcct caggtttttg
    19141 aatccgttaa agaagaatat ttcacagtca aacttgctgc attaaaaaaa cataaaagcc
    19201 aggaacgacg tgattatatg cgtcatgatc gcctgcgtgc agttgcacaa tttcgcgggc
    19261 aacaggtcaa tagcgatctg ggtgaaggct ttgtaattca taaaatgatt ctttgagcaa
    19321 attgcatgaa aacctggata tttatctgta tgtccatagc aatgttgcta tggtttttaa
    19381 gtacgctaag acgtaaaccc agtcaaaaga aaggctgtat tgacgccatt atacctgcgt
    19441 ataacgaagg cccgtgtctg gcgcagtcac tggataatct actgcgtaac ccttattttt
    19501 gccgggtaat ttgcgttaac gacggctcca cggacaatac cgaagcggtc atggcggaag
    19561 tcaaacgcaa atggggcgac cgctttgttg ccgtcacgca aaaaaatacc ggtaaaggtg
    19621 gtgcgctgat gaatggcctc aattatgcca cctgcgacca ggttttttta agtgatgccg
    19681 acacctatgt tccgcccgat caagacggaa tgggctatat gctggcagaa attaagcgcg
    19741 gtgctgatgc cgtaggcggc attccctcta ctgcgttgaa aggcgcgggg ctgttaccgc
    19801 acatccgcgc gaccgtaaag ttgccgatga ttgttatgaa gcgcacgcta cagcagctcc
    19861 tgggcggcgc accgtttatt atcagcggtg cctgcggaat gttccgtact gatgtattgc
    19921 gtaagttcgg tttctcggat cgtactaaag tcgaagacct tgatctcacc tggacattag
    19981 tggcaaacgg ctaccgtatt cggcaggcga atcgctgcat cgtataccca caggaatgca
    20041 acagtccgcg tgaggagtgg cgtcgctggc ggcgttggat tgtgggctac gcggtctgta
    20101 tgcgcctgca taaaaggctt ttatttagcc gcttcggtat cttcagtata tttcctatgc
    20161 tgttggttgt gatttatggc gttgggattt atctcactac ctggtttaat gaattcatca
    20221 ccaccgggcc gcatggtgtg gtgttggcaa tgtttccgct tatctggatc ggcgtagttt
    20281 gtgttattgg tgcttttagc gcctggtttc atcgttgctg gttgttggtg cctttagcgc
    20341 cgctttccgt tgtgtatgta ttattagctt atgccatctg gattatctat ggacttattg
    20401 ccttttttac tggacgcgaa cctcagcgcg acaaacccac ccgctattcc gcactggtgg
    20461 aagcgtcaac cgcttattcc caaccttctg tcacaggaac tgaaaaacta tctgaagctt
    20521 aatgttaaag agaaaaatta ttcagattgc agaccaggta attattgatg aaactgcagg
    20581 tgaagttgtt atcggcgcga atacccgtat ttgtcatggt gccgttattc agggtccggt
    20641 agtgattggc gcaaactgcc tgataggtaa ttatgcgttt attcgtcctg gcacaataat
    20701 cagcaatggt gtaaaaattg gttttgccac cgaaattaaa aatgcggtta ttgaagcgga
    20761 agcaacgatt ggtccgcaat gttatattgc cgactcggta gttgcaaacc aggcatattt
    20821 gggcgcacaa gtacgtacca gtaatcatcg tctggatgaa cagcctgtgt ctgttcgaac
    20881 tccagaggga attatcgcta ccggatgcga taaattaggt tgttatatcg ggcagcgttc
    20941 acgccttggc gtacaagtta ttattttgcc tgggcgaatt atttccccga acacgcaact
    21001 tggcccgcgc gtgattgtag aacgtaattt acctaccgga acttattcac tccgacaaga
    21061 acttatccgt acaggagatt aaaatgataa aacggacgtt attagcggcg gccattttta
    21121 gcgcattgcc cgcttatgcc gggttaactt ccattaccgc gggctacgat tttaccgatt
    21181 attctggcga tcatggcaac cgtaatttag cgtatgctga actggtggcg aaagttgaaa
    21241 acgcaacgct gctttttaat ctttcccagg ggcgtcgtga ttatgaaact gaacatttca
    21301 atgccactcg cggtcagggt gcggtctggt ataaatggaa taactggctg acaacccgaa
    21361 cgggtattgc ctttgcggat aatacgccgg tctttgcccg ccaggatttt cgtcaggata
    21421 ttaacctggc cctgttgcca aaaacgcttt tcacgaccgg ttatcgctac actaaatatt
    21481 acgatgatgt cgaagtcgat gcctggcaag gcggcgtatc actctatact ggcccggtga
    21541 tcaccagcta ccgctatacc cattatgact ccagcgatgc aggtggtagt tatagcaata
    21601 tgatttccgt gcgtctgaat gacccgcgcg gcactggtta tacgcaacta tggctaagcc
    21661 gcggaacagg cgcttacacc tatgactgga cgccagaaac acgctacggc agcatgaaga
    21721 gcgtcagttt gcaacgtatt caaccgctga ctgagcaact taatctcggg ctgacggcag
    21781 gtaaagtgtg gtacgacacc ccaaccgatg attataacgg tctgcaactt gcagcccatc
    21841 tgacctggaa attctgattg cttctgccgc ccgctatccg gggcggcctt ccctgccgat
    21901 tagccccact ccctttcctc gttgttttcc gaccacattc accggataaa ttttattctc
    21961 cagcgttata tactataggg aggtatgcat tgacatatag catacctccc tatagtatat
    22021 tgcgtgcaga tgatgaggtg cgaaatgccc agtactccgg aagagaagaa aaaggtcctt
    22081 actcgagttc gtcgtattcg ggggcagatt gatgctctgg aacggtcgct ggagggtgat
    22141 gccgaatgcc gtgccatact ccaacagatc gctgccgttc ggggcgcggc taacgggctg
    22201 atggcagaag tgcttgaaag ccatatccgg gaaacgtttg accgaaatga ctgctacagc
    22261 cgcgaagtca gccaatccgt tgacgacact attgaactgg ttcgagccta tcttaaatag
    22321 ctgaatctat taccatattg aggaagagcg agagatgaaa tcacgtgctg ccgttgcatt
    22381 tgctcccggt aaaccgctgg aaatcgttga aattgacgtt gcaccaccga aaaaaggtga
    22441 agtgctgatt aaagtcaccc ataccggcgt ttgccatacc gacgcattta ccctctccgg
    22501 ggatgacccg gaaggtgtat tcccggtggt tctcggtcac gaaggggccg gcgttgtggt
    22561 tgaagtcggt gaaggcgtaa ccagcgtcaa acctggcgac catgtgatcc cgctttacac
    22621 cgcggagtgc ggcgagtgtg agttctgtcg ttctggcaaa actaacctct gtgttgcggt
    22681 tcgcgaaacc cagggtaaag gcttgatgcc agacggcacc acccgttttt cttacaacgg
    22741 gcagccgctt tatcactaca tggggtgctc tacattcagt gaatacaccg tggtcgcgga
    22801 agtgtctctg gccaaaatta atccagaagc aaaccatgaa cacgtctgcc tgctgggctg
    22861 tggcgtgacc accggtattg gcgcggtgca caacacagct aaagtccagc caggtgattc
    22921 tgttgccgtg tttggtcttg gcgcgattgg tctggcagtg gttcagggcg cgcgtcaggc
    22981 gaaagcggga cggattatcg ctatcgatac caacccgaag aaattcgatc tggcgcgtcg
    23041 cttcggtgct accgactgca ttaacccgaa tgactacgac aaaccgatta aagatgtcct
    23101 gctggatatc aacaaatggg gtatcgacca tacctttgaa tgcatcggta acgtcaacgt
    23161 gatgcgtgcg gcgctggaaa gtgcgcaccg cggctggggt cagtcggtga tcatcggggt
    23221 ggcaggttct ggtcaggaaa tctccacccg tccattccag ttggtcactg gtcgcgtatg
    23281 gaaaggttcc gcgtttggcg gcgtgaaagg tcgttcccag ttaccgggta tggttgaaga
    23341 tgcgatgaaa ggtgatatcg atctggaacc gtttgtcacg cataccatga gccttgatga
    23401 aattaatgac gccttcgacc tgatgcatga aggcaaatcc attcgaaccg taattcgtta
    23461 ctgatttccc gcaggtatac cccgtccact tcagacgggg ttcttaatac tctccccggg
    23521 cagccgtccg ggggattaac cgtgagataa tgactgatgg aactcattga aaaacatgcc
    23581 agctttggcg gctggcaaaa tgtgtatcgg cattattccc aatcactgaa atgtgaaatg
    23641 aatgtcggcg tctatctccc accaaaagcc gcgaatgaaa aattgccggt gctgtactgg
    23701 ctttcaggcc tgacctgcaa cgagcagaat ttcattacta aatcggggat gcagcgttac
    23761 gcggctgagc acaacattat tgttgttgcg ccggacacca gtccgcgagg cagtcatgtc
    23821 gcagatgccg accgttacga tctcgggcaa ggtgccgggt tttacctgaa cgcaacgcaa
    23881 gcgccgtgga atgaacatta caaaatgtat gactatatcc gcaacgagct gccggattta
    23941 gtgatgcaac attttccggc aacgaccaga aagtctatct ctggtcattc tatgggcggg
    24001 ctgggcgcgc tggtgctggc gttacgtaac ccagatgaat atgtcagcgt ctcggcgttt
    24061 tcgcccattg tctccccatc gcaagtaccg tggggacagc aagcctttgc cgcatatctt
    24121 ggtgaaaata aagatgcctg gttggattac gatccggtga gtcttatttc acaaggtcaa
    24181 cgcgttgcgg aaatcatggt tgatcagggg ttgagtgatg atttttacgc ggaacagctg
    24241 cggactccaa atcttgaaaa gatctgccag gagatgaata tcaagacgtt aatccgttat
    24301 cacgagggtt acgaccacag ctattatttt gtctccagtt ttattggcga gcatattgcc
    24361 taccacgcca ataaactgaa tatgcgttga taatagtgca cgactgccgg atgcggcgtg
    24421 aacgccttat ccggcctaca cttcgcccgt aaaccgtagg cctgataaga cgcgcaaagc
    24481 gtcgcatcag gcatggcgca cgactgccgg atgcggcgtg aacgccttat ccggtctaca
    24541 cttcgcccat aaaccgtagg cctgataaga cgcgcatagc gtcgcatcag gcatctgcgc
    24601 acgacttatc tatgtttacc acatcaaatc atcaggcact ttgaagtcgg catacggatc
    24661 gtcttcatcc tgctcttcgg cactgagcgc gctgtgcaac acaatactgc tggcatcacg
    24721 ctgagcaatt ttatcggcaa cactggcggg aataatggtg tattcacctt cgctattgtt
    24781 atcgaccaac aagcgggcaa tcgccagacg accattaatc aactgcgcct gtgtgagctt
    24841 atcgacaaaa atcttcttaa tcagattgcc gtcagtgaag ttaaaaccaa tatcgccatt
    24901 ggcaatggtg attcggttca tttcaatgag ctgcttcacc tgggctttat attctttcgc
    24961 caacgccgct tgtttttgct gttcgctaag ctgtttatca cgctcaagct gtgccttttt
    25021 attttcttct accgcagccc gagcttcacg cgcctgaacg cgcgattttt tcgccgttct
    25081 ctcgaccttc gccgcttttt tgctggtgac taatcctgct ttgagcaact gctcttgtaa
    25141 ggtaagtttt gccatttttg tttccaaacc cgctgaataa tttttagcga gtatacctgt
    25201 aaacgccgtg ggtgtattgg gggcaagtcc ctttcgcccc tgcttaataa ggcgcgtcag
    25261 gcatcggcac acggctgcat tcttgatctc cgactcatca aaataaacac cgccaaccca
    25321 gcaacaagaa tacccggtgc agaagcggcc attacgccga ccgtgccagt gcctaatgcc
    25381 agcattttcc cggccagtaa cggaccgctc atagccccca gacgccctac cgccacggct
    25441 gttcccacac ctgttgcgcg gatctgcgaa ctgtaaaaca acggtgccag ggcatacaaa
    25501 acgctttgcc cacctgtcgc aaacaacccc gcgacaaatc ccgccagcaa cataccgtta
    25561 aatgacgaca ccgttccaag cgccagcagc gaagctaaca tgccgctata aatcagtagc
    25621 gacatggtta ctggacgcag cttatccatc aatgcgccca acattaacgt cccgcttgcc
    25681 gcccccattt gcagggcaaa catcacccct gccgcctgcg atggctggaa tccttgctcc
    25741 accaaaagta gcggtagcca gttgatcaac atataaacca ccagcagagt gaagaaataa
    25801 cacaaccaca gcagcagcgt cgcggttgcc gtttctggcg caaataaggc acgcagtggt
    25861 ggcgcagcct gtttttcgcc agcgaaaacc gctgactccg gcagccagcg cattaatagc
    25921 ggcaccagaa tcaacggcac cacaccacct acccaaaaca ccgtttgcca tgctaagttt
    25981 gcccccgcga aacccagtgt cgccgccagc gccgcgccaa tgggaacacc gcaatacatc
    26041 aggctcactg ccgtcccacg aaaacgtgga cccgcgcttc agatgtcagg gcgataagat
    26101 tcggcaacgc cgcccccagc ccgacaccgg tcatcagccg cgcaaagacc agtgagggga
    26161 aatcccaggc aatcgccgtt gccagtgaga acaaaccaaa cagcgcaact gagccaatca
    26221 aaatacgctt acgaccataa cggtctgcca gcatcccgcc aaccaacgcg ccgggtagca
    26281 aaccgagtat tccggcgcta aatatccagc ccatttgcat tttatcgagt gcgaaagcct
    26341 gggcgatgcc acccgccgca atgccagccg cctgaagatc cagcccttcc atcagagcga
    26401 ccaaaaaaca aagcccgatg gtcagcatca ggcgggatga agatgatgaa ggggtacgag
    26461 tcgacataag atgaacctca ttgcgagtgc aggtgaatcg cgactgatac attgccagcc
    26521 gcgtaatcag aactatttaa tgactgccta cgggcgaaat gtagcgaaat gtaggccgga
    26581 taaggcgttc acgccgcatc cggcagtcgt gcgcagatgc ctgatgcgac gctaacgcgt
    26641 cttatcagac ctacaaattc tcacgccatc cgtaggccgg acaaggcgtt cacgccgcat
    26701 ccggctgtcg tgctgggatg ggcatacaaa atgcacgtta tcggcagtca tcattatttg
    26761 ttgttgcgca gatccagcgc cacgtcgacg atcatatcct cctggccgcc aaccatccgg
    26821 cgtttgccca gctcaacgag aatatccacc gcacttaagc cataacgcgc cgccgccgtt
    26881 tcacagtgac gcaggaagct cgagtaaaca ccagcgtatc ccagcgccag cgtttcgcga
    26941 tcgactcgta ccggacgatc ctgcaacgga cgcaccaggt cgtcggcggc atccattaac
    27001 gcatagagat cggtcccatg ctgccagccc agtttatccg cggcggcaat aaacacttcc
    27061 agcggtgcgt tacctgcgcc cgcgcccatt cccgcgaggc tggcgtcgat tcggtcgcag
    27121 ccctcttcca ccgccgcgat agagttcgcc acgccaagac tcaggttatg gtgagcgtgc
    27181 atgccggttt gcgtttctgg tttcaacact gctttcaggg cgcggaaacg gtcacggata
    27241 tcgctcatgt tcatcgcacc gccagaatcc accacataaa tacaggtcgc accgtagcct
    27301 tccatcagct ttgcctgctt ggcgagattc tccggcgtgg tcatatggct catcatcaga
    27361 aaaccaacgg tgtccattcc gagctcgcgg gcatactgaa tatgctgggc ggaaacatca
    27421 gcttcggtac agtgcgttgc cacacgaacc acccgcgcgc cagcctgcca ggcatttttc
    27481 agatcgtgaa tagtgccgat tcctggcagc aacaacgtcg cgattttggc gtgcctcacc
    27541 acatccgccg ccgcttcaat ccattcaagg tcgctatgtg cgccgaaacc atagttaaag
    27601 ctggaacctt gcaaaccgtc gccgtgggcc acttcaatcg aatccacgcg ggcatcgtcc
    27661 agtgctttgg caatctggcg aacgttttcc agcgaatact gatgacgaat ggcgtgcata
    27721 ccgtcacgca atgtgacgtc cgagatataa agttttttac cgttcatgcc gcttctcctg
    27781 ccttgcgcgc cagtgactgg gccatttttt ccgctgtcgc cagcgcactg gaagtcataa
    27841 tatcgaggtt gcccgcatag gcaggcagat aatgcgctgc gccttcgact tccagccaga
    27901 ccgctgtttt cagtccggag aattgcccca cgcccggtaa attgaccggt ttatcctgcg
    27961 ggataacttc aaactgcacg cgctgtttca ggcgataacc cggtacgtaa gcctgcaccg
    28021 cctcagccat ttcattgatt gaggcttcga tatcatcttg tgaagcttcg tcgctcaata
    28081 catacaccgt gtcacgcatc atcaacggtg gctctgctgg gttaagcaca ataatcgcct
    28141 tcccttttgc cgcgccgccc accacttcaa tggctcggga agtggtttcc gtaaattcat
    28201 cgatattggc acgcgtgcca ggtccggcag atttactggc gatagaagcg ataatttcgg
    28261 cgtaatgaac acgcgccacg cgtgaaactg ccgccaccat tggaatggtg gcctggccgc
    28321 cgcaggtgac catgttgacg ttcagttgat cgacgttcgc ctcgaggtta accaccggca
    28381 cgcagtaagg gccgatggca gcaggcgtca ggtcaattaa gcgaatatcc ggtttcgctt
    28441 cgcgtaaagc ggcatcgttt ttcacatgcg cgcccgcgct ggtcgcatca aatacaatgt
    28501 cgatatcagc aaattcaggc atgttcatca gcccgatcac cccttcatgg gtggtggcga
    28561 cgcccatacg tctggcacgc gccagaccgt cggactgagg atcaatgccg accatcaccg
    28621 ccatctccag atgctgaccg tgacgcaaaa ttttaatcat cagatcggtg ccaatgttgc
    28681 cagaaccgat aatggcgact ttacgcttac tcatgacaga cttccttttg gggctgcgct
    28741 tgaaaatgtc gcagcaactg aacctatgcc ttcaatatgg gcttcaaaac gatcgcccgc
    28801 attcaccgcc accatcgggc ctaatgcccc ggtaagaatg atatctccgg cgcgcagcgg
    28861 ttcacccaga ctggccattt tgcgtgccag ccagacggcg gcattaagcg gatgtcccag
    28921 gcattcgctg ccgcgcccgc tggaaacctc ttcgttatta cgcgtcatct tcatggcgca
    28981 gtttttcagg tctaaccccg ccggacgttg cgccggaccg ccgatgacat acaccccgca
    29041 ggaagcgtta tctgccacgg tatcgacaaa ctgaatcgac cagtcgcgaa tgcggctccc
    29101 caccacttcc agcgccggaa gtacccattc aatggcgtta tacagttcgt cgaaggtgat
    29161 atcagttgcg ggtaaatcgc ggttcaacac cagtgcgatc tccgcttcaa tgcggggttg
    29221 cagaacacgg gaaaaaggaa tgatttcgtt atcgccataa cacatgtcgg caaataacgt
    29281 cccaaaatcc ggttgatcaa cgcccagttg ttgttgcact ttcggatgcg tcaggcccac
    29341 tttacgccct accacgcgac gcccctgcac aacgtcatat tgcacattta tgtgctgaat
    29401 ggcgtaggcc gcttcagcgt gatcgatacc aatcagatcg cgcagcggcg caatcgcttc
    29461 gccctgctct gcggcgcggc gtaaatccgc cgccagttgc tcaagagtat gcttcgtcat
    29521 gaccattcct taagggcgtg cgaggaaatt cagcaccaat tgattgaaag cgtcggcatg
    29581 ttcccactgc gcccagtgac cacagtcgcg gaagatatgc agttcagaac cggcaatgcc
    29641 ggacagcaga cgcagacccg catccatcgg cacaaagcgg tcgttgcgcc cccagacaat
    29701 cagggtttgc gctttgattt ccgccagacg tgggccaaaa tccgggaact gtttcggatt
    29761 agcttccagg ctcttaacga agttttccag gtgatcgcgg cgcgacagca tattattcag
    29821 gcgcgcttca aacagggcgt cggtcaaatc gctggtatcg aaaacgaaga tatccatcat
    29881 cagcttcagg ttttcgatag tcggctgacg ataaagctga ttcagtcgct taataccttc
    29941 ggttggcatc ggcgtaaaca aactcatgcc gcccgtaccg ccgcccatca gcaccagttt
    30001 gccgacgcgc tccggccagt taagggtgaa cgccacagaa ctatggccgc ccatcgagtt
    30061 gcccagcagg tggattttgg cgatatccag ttgatccacc acgcttttca ggattcgtgc
    30121 attaagatcc gatcgtgaac cactattaac gatcgaatcg cttttgcccc aacccggaca
    30181 atccagcagg atcacccgat agcccgcctc taccagcgga tcgatattgc ggctgaagtt
    30241 cgcccagcca gtagcacccg ggccggaacc atgcagcagg acaacggttt cgtcgccttg
    30301 tccgcagtca ttaaaatgga tgcgcagcgt tttacccgct tcttctacat tcagaaaacg
    30361 gctggtggcg gcttcggttt gtggctgata actcatcatc ttctcctgca tattcagttc
    30421 tctgttctgg cgcttaacga gccaaatccg gcaatccact ccgggattgg gcggtaataa
    30481 cgcccttcgc tacgccagtt gccaaatgca gaaatagcgg caaaagcggc gacccaggtt
    30541 ttgatttcat gtgtcgactt tccggcaatg gcggaaagct cttcgttact gacggcatcc
    30601 agttcctgta tgcgtccctg ctccagcaaa gtcatgaact ggttatccca aatcgggttg
    30661 agcggatgca gagttctctg atcctcaaca aacttctcag cggcgctaat caccctttgc
    30721 tgacgcaatt cgcgctcact ggcgggtaaa tctttcccgc tccccaacag acggtcgcgc
    30781 atatgggcat cggctttcgc cagttcgggc accggcggct gatgggaaag cccaccggaa
    30841 cccaggaaca gcacgcgttt attgagagtg ctggtgaaac gtccaatggc ttcacccaac
    30901 atgcgggtac gctggaaacc gggcagcggc gtggcgacac cgttgatgaa cacaggcaga
    30961 actggcacct tatccagccc accgagcagg aactccagcg gctgggcgaa cccgtggtcc
    31021 acctgcatac agtaagaaac ggcaagatcg atcccgctct tcatgacagc atgcgcacag
    31081 gcctccgcca gctccacagg cacgggcagc tctcctgccg cactgccgaa atcaccaatt
    31141 gccgtcgctc caacgcctaa acagaacggt ggcatcacgt catagaaaaa gccgttgtag
    31201 tgatctggcg caaacagcac caccagttca ggggagaaag ccgcaatacg ctcgcgggcg
    31261 ctggcaatca cgccattgac ctcatcgagt acctcttgcg ccgggtcgac ataccccacc
    31321 aacggcgagt gggaaagaca gtgaagataa gcgtgcatat caggctacct tttcgacaga
    31381 aacgtcggca tcagggcggg tcagcgtcat caccgacgcc agtttattca gcgtattgcc
    31441 cagggtttgc ggaatggcgg tggcggcaac aaagcgatcc gggcgcatca ccaccagcga
    31501 agcattgtgt tgcgcgaacc agctacgcag acgaccttgc gtatcgccca cgtgtagcac
    31561 gccgtcgtgg ttatcctgtg cggtatgaat ttgcacttcc ggcaccacct gaatgaagcg
    31621 tgtgcccaac gcgcgccact gctggatttg ctcgtcgctc atcccccaca gtggattgca
    31681 tccccagcca attaccgcga agttcgcgcc gatcgcgtta tcgagcagcg tcacgtcgcc
    31741 gttttccagc gtgactttcg gctgaataaa catcttgccg accggagagt gcttcgcttc
    31801 gccctcacgc accagcgcac cgccgtaata ttgcggcatc ggcttgaagc gcatttcgag
    31861 gaagtagcgt tttactggcg gcagataatt caacagccag gaaacgccgt cacgtaacgt
    31921 accctgccag cgtttcggcg gagccagcac gttgcccgcc gtcacggaca ggtcaatcat
    31981 cgctttggcg tgatcgcgac gttcttgttg ataggtatcg agcagcgcat cgcgggcttt
    32041 cccctggata accaacgcca gtttccaggc gaggttaaag gcgtcgcgca taccactgtt
    32101 atagccctgc ccctgccaca ccggcatgat gtgcgcggca tcgcccgcca gcagtacgcg
    32161 atcaatacgg aaacgttgcg ccagtcgcgc gttatgggtg tagacacgct ggcgaatcaa
    32221 ttcaacattg tccggattag gcagcacttt gcttaacagc ttgcgcatat tttgcggctc
    32281 acgcagctgc tcttcggttt ctcccggcat caccataaat tcaaagcgac gtaccgcatg
    32341 aggcagcgcg gcagaaacat acgggcgcac cggatcgcaa cacaaataga tatgcggcgt
    32401 acttaacgga tcgttggcga tatctaccac aatccactga tttggcgcag ttttaccttc
    32461 aaacggcaca ttcagggtgc gacgaacaaa acttgcccca ccatcacagg ccaccagcca
    32521 ctgggctttg atcgtttccc gctgcccttc tgccgttttc aggtgcaagg tcacttcgtc
    32581 atcttgctgg ctgaaggcct ccagctcgcg ggaaaacaag cagcgcacat tcggaaaacg
    32641 cgacaaccct tccagcatca ccgcatcgac ctgtggctga ataaaggcgt tacggcgcgg
    32701 ccagccaaat tcatcggtca ttggctgaat atcagcaaaa cagcggcctt ttggggtgag
    32761 aaaacgcatc gcgtgccacg gtgtagtgtg cggcagaaca ttctcgacca ggccgaccga
    32821 ctgcatggtg cgcagcgcct cgtcatcaat accaatcgca cgcgggtagt cgatcaactt
    32881 atcgagtttc tccaccacca gcacgtcaat gcccatctga ccgagatagt tcgccatcat
    32941 cagcccaacc ggaccggcac cagcgatcgc cacctgaacg ctatggttaa cagcaggctg
    33001 gatgtcaggg tgttgtattg ccatttctgc acaacgttgt tttaactata gaaatgtcaa
    33061 ttaatatgca gaacaatgag cagacggaat acaaaaccgt gcgcggctta acccgcggtc
    33121 taatgttatt aaatatgtta aataaacttg atggcggtgc cagcgtcggg ctgctggcgg
    33181 aactcagcgg cctgcatcgc accactgtgc ggcgactgct ggagacgctg caggaagagg
    33241 gatatgtccg ccgtagcccc tccgatgaca gttttcgtct gaccatcaaa gtgcggcaat
    33301 taagcgaagg atttcgtgac gaacagtgga tttctgcact ggcggccccg ctgctgggcg
    33361 atctgttgcg cgaagtggta tgaccaacag atgtgtccac cctggatgtt gatgcaatgg
    33421 tggtacgcga aaccacccac cgtttcagcc gcttatcctt tcaccgggca atggtcgggc
    33481 gacgtttacc gctcctgaaa accgcctcgg gcctgacctg gctggccttt tgcccggaac
    33541 aagaacgcaa ggaattaatc gaaatgttag ccgcccgccc cggtgatgac tatcaactgg
    33601 cacgagaacc gttaaagctg caagccattc tggcgcgcgc gcgcaaagag ggttacggac
    33661 agaactaccg cggctgggat caggaggaga agatcgcctc tatcgccgta ccgctgcgca
    33721 gtgaacaacg ggtgattggc tgtctgaatc tggtgtatat ggcgagcgca atgaccattg
    33781 aacaggcagc ggaaaagcat cttccggcgc tacaacgggt agcaaaacag atcgaagaag
    33841 gggttgaatc gcaggctatt ctggtggccg gacggcgaag cggcgtgcat ttacgttgac
    33901 accatcgaat ggcgcaaaac ctttcgtggt atggcatgat agcgcccgga agagagtcaa
    33961 ttcagggtgg tgaatgtgaa accagtaacg ctatacgatg tcgcagagta tgccggtgtc
    34021 tcttatcaga ccgtttctcg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg
    34081 gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa
    34141 ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg
    34201 ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg
    34261 gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc
    34321 gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct
    34381 gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc
    34441 atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc
    34501 gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt
    34561 ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa
    34621 cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag
    34681 ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc
    34741 gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat
    34801 accgaagaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg
    34861 ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc
    34921 aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa
    34981 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
    35041 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
    35101 ccaggcttta cactttatgc ttccggctcg tatgttgtgt gaaattgtga gcggataaca
    35161 atttcacaca ggaaacagct atgaccatga ttacggattc actggccgtc gttttacaac
    35221 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatca ccttgcagca catccccctt
    35281 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
    35341 gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg ccggaaagct
    35401 ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc
    35461 acggttacga tgcgcccatc tacaccaacg tgacctatcc cattacggtc aatccgcctt
    35521 ttgttcccgc ggagaatccg acgggttgtt actcgctcac atttaatatt gatgaaagct
    35581 ggctacagga aggccagacg cgaattattt ttgatggtgt taactcggcg tttcatctgt
    35641 ggtgcaacgg gcgctgggtc ggttacggcc aggacagccg tttgccgtct gaatttgacc
    35701 tgagcgcatt tttacgcgcc ggaaaaaacc gcctcgcggt gatggtgctg cgctggagtg
    35761 acggtagtta tctggaagat caggatatgt ggcgaatgag cggcattttc cgtgacgtct
    35821 cgttgctgca caaaccgagc acacaaatca gcgatttcca tgttgccact cactttaatg
    35881 atgatttcag ccgcgcggta ctggaggcag aagttcagat gtacggcgag ctgcgcgatg
    35941 agctgcgggt gacggtttct ttgtggcagg gtgaaacgca ggtcgccagc ggcaccgcgc
    36001 ctttcggcgg tgaaattatc gatgagcgtg gtggttatgc cgatcgcgtc acgctacgtc
    36061 tgaacgtcga aaacccggcg ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg
    36121 ttgaactgca caccgacgac ggcacgctga ttgaagcaga agcctgcgat gtcggtttcc
    36181 gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg caagccgttg ctgattcgcg
    36241 gcgttaaccg tcacgagcat catcctctgc atggtcaggt catggatgag cagacgatgg
    36301 tgcaggatat cctgctgatg aagcagaaca actttaacgc cgtgcgttgt tcgcattatc
    36361 cgaaccatcc gctgtggtac acgctgtgcg accgcttcgg cttgtacgtg gtggatgaag
    36421 ccaatattga aacccacggc atggtgccaa tgaatcgtct gaccgatgat ccgcgctggt
    36481 tacccgcgat gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg
    36541 tgatcatctg gtcgctgggg aatgagtcag gccacggcgc taatcacgac gcactctatc
    36601 gctggatcaa atctgtcgat ccatcccgcc cggtgcagta tgaaggcggc ggagccgaca
    36661 ccacggccac cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc
    36721 cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct acctggagag acgcgcccgc
    36781 tgatcctttg cgaatacgcc cacgcgatgg gtaacagtct tggcggcttc gctaaatact
    36841 ggcaggcgtt tcgtcagtac ccccgtttac agggcggctt cgtctgggac tgggtggatc
    36901 agtcgctgat taaatatgat gaaaatggca atccgtggtc ggcttacggc ggtgattttg
    36961 gcgatacacc gaacgatcgc cagttctgca tgaacggtct ggtctttgcc gaccgcacgc
    37021 cgcatccagc gctgacagaa gcaaaacacc agcagcagtt tttccagttc cgtttatccg
    37081 ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca tagcgataac gagcttctgc
    37141 actggtcggt ggcgttggat ggcaagccgc tggcaagcgg tgaaatgcct ctggatgttg
    37201 ctccacaaga taaacagttg attgaactgc ctgaactacc gcagccggag agcgccggac
    37261 aactctggct tacggtacac gtagtgcaac cgaacgcgac cgcatggtca gaagccggac
    37321 acatcagcgc ctggcagcag tggcgtctgg cggaaaacct cagcgtgaca ctccccgccg
    37381 cgccccacgc catcccgcaa ctgaccacca gcgaaacgga tttttgcatc gagctgggta
    37441 ataaacgttg gcaatttaac cgccagtcag gctttctttc acagatgtgg attggcgctg
    37501 aaaaacaact gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg gataacgaca
    37561 ttggcgtaag tgaagcgacc cgcattgacc cgaacgcctg ggtcgaacgc tggaaggcgg
    37621 cgggccatta ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca cttgccgacg
    37681 cggtgctgat tacgaccgct cacgcgtggc agcatcaggg aaaaacctta tttatcagcc
    37741 ggaaaaccta ccggattgat ggtagtggtc aaatggcgat taccgttgat gttgaagtag
    37801 cgagcgatac accgcatccg gctcggattg gcctgacctg ccagctggcg caggtagcag
    37861 aacgggtaaa ctggctcgga ttagggccgc aagaaaacta tcccgaccgc cttactgccg
    37921 cctgttttga ccgctgggat ctgccattgt cagacatgta taccccgtac gtcttcccga
    37981 gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta tggcccacac cagtggcgcg
    38041 gcgacttcca gttcaatatc agtcgctaca gccaacaaca actgatggaa accagccatc
    38101 gccatctgct gcacgcggaa gaaggcacat ggctgaatat cgacggtttc catatgggga
    38161 ttggtggcga cgactcctgg agcccgtcag tttcagcgga attccagctt agcgccggta
    38221 gctaccatta ccagttggtc tggtgtcaaa aataataata accgggcagg ccatgtctgc
    38281 ccgtatttcg cgtaaggaaa tccattatgt actatttaaa aaacacaaac ttttggatgt
    38341 tcggtttatt ctttttcttt tactttttta tcatgggggc ctacttcccg tttttcccga
    38401 tttggctaca tgacatcaac catatcagca aaagtgatac gggtattatt tttgccgcta
    38461 tttctctgtt ctcgctatta ttccaaccgc tgtttggtct gctttctgac aaactcgggc
    38521 tgcgcaaata cctgctgtgg attattaccg gcatgttagt gatgtttgcg c
//
LOCUS       DAESHI010000032        38337 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.032, whole genome shotgun sequence.
ACCESSION   DAESHI010000032 DAESHI010000000
VERSION     DAESHI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.032"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            59..1423
                     /locus_tag="KW011_001658"
     CDS             59..1423
                     /locus_tag="KW011_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH7660014.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="KW011_001659"
     CDS             complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="KW011_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HBH7660015.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2210..3121
                     /gene="panE"
                     /locus_tag="KW011_001660"
     CDS             2210..3121
                     /gene="panE"
                     /locus_tag="KW011_001660"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBH7660016.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3084..3674
                     /gene="yajL"
                     /locus_tag="KW011_001661"
     CDS             3084..3674
                     /gene="yajL"
                     /locus_tag="KW011_001661"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HBH7660017.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="KW011_001662"
     CDS             complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="KW011_001662"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBH7660018.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5382..5624
                     /gene="xseB"
                     /locus_tag="KW011_001663"
     CDS             5382..5624
                     /gene="xseB"
                     /locus_tag="KW011_001663"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBH7660019.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLPPFTPDNE"
     gene            5624..6523
                     /gene="ispA"
                     /locus_tag="KW011_001664"
     CDS             5624..6523
                     /gene="ispA"
                     /locus_tag="KW011_001664"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HBH7660020.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6548..8410
                     /gene="dxs"
                     /locus_tag="KW011_001665"
     CDS             6548..8410
                     /gene="dxs"
                     /locus_tag="KW011_001665"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBH7660021.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8465..9439
                     /gene="yajO"
                     /locus_tag="KW011_001666"
     CDS             8465..9439
                     /gene="yajO"
                     /locus_tag="KW011_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HBH7660022.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="KW011_001667"
     CDS             complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="KW011_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBH7660023.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="KW011_001668"
     CDS             complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="KW011_001668"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HBH7660024.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="KW011_001669"
     CDS             complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="KW011_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBH7660025.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="KW011_001670"
     CDS             complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="KW011_001670"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBH7660026.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="KW011_001671"
     CDS             complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="KW011_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBH7660027.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="KW011_001672"
     CDS             complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="KW011_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HBH7660028.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13749..14288
                     /locus_tag="KW011_001673"
     CDS             13749..14288
                     /locus_tag="KW011_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBH7660029.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14587..15471
                     /gene="tsx"
                     /locus_tag="KW011_001674"
     CDS             14587..15471
                     /gene="tsx"
                     /locus_tag="KW011_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HBH7660030.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15648..15995)
                     /locus_tag="KW011_001675"
     CDS             complement(15648..15995)
                     /locus_tag="KW011_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HBH7660031.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16124..17095)
                     /gene="secF"
                     /locus_tag="KW011_001676"
     CDS             complement(16124..17095)
                     /gene="secF"
                     /locus_tag="KW011_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HBH7660032.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17106..18953)
                     /gene="secD"
                     /locus_tag="KW011_001677"
     CDS             complement(17106..18953)
                     /gene="secD"
                     /locus_tag="KW011_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HBH7660033.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="KW011_001678"
     CDS             complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="KW011_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBH7660034.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="KW011_001679"
     CDS             complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="KW011_001679"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBH7660035.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20519..21589)
                     /gene="queA"
                     /locus_tag="KW011_001680"
     CDS             complement(20519..21589)
                     /gene="queA"
                     /locus_tag="KW011_001680"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBH7660036.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21682..22263
                     /gene="acpH"
                     /locus_tag="KW011_001681"
     CDS             21682..22263
                     /gene="acpH"
                     /locus_tag="KW011_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HBH7660037.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="KW011_001682"
     CDS             complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="KW011_001682"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HBH7660038.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(24241..25614)
                     /gene="proY"
                     /locus_tag="KW011_001683"
     CDS             complement(24241..25614)
                     /gene="proY"
                     /locus_tag="KW011_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HBH7660039.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="KW011_001684"
     CDS             complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="KW011_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HBH7660040.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="KW011_001685"
     CDS             complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="KW011_001685"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HBH7660041.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="KW011_001686"
     CDS             complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="KW011_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HBH7660042.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29648..30850
                     /gene="sbcD"
                     /locus_tag="KW011_001687"
     CDS             29648..30850
                     /gene="sbcD"
                     /locus_tag="KW011_001687"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HBH7660043.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30847..33990
                     /gene="sbcC"
                     /locus_tag="KW011_001688"
     CDS             30847..33990
                     /gene="sbcC"
                     /locus_tag="KW011_001688"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HBH7660044.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLTLENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34116..35300
                     /gene="araJ"
                     /locus_tag="KW011_001689"
     CDS             34116..35300
                     /gene="araJ"
                     /locus_tag="KW011_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HBH7660045.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35443..36351)
                     /gene="mak"
                     /locus_tag="KW011_001690"
     CDS             complement(35443..36351)
                     /gene="mak"
                     /locus_tag="KW011_001690"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HBH7660046.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36476..37387
                     /gene="rdgC"
                     /locus_tag="KW011_001691"
     CDS             36476..37387
                     /gene="rdgC"
                     /locus_tag="KW011_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HBH7660047.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37465..37920)
                     /locus_tag="KW011_001692"
     CDS             complement(37465..37920)
                     /locus_tag="KW011_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660048.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37917..38252)
                     /locus_tag="KW011_001693"
                     /pseudo
     CDS             complement(37917..38252)
                     /locus_tag="KW011_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
BASE COUNT         9465 a        10313 c         9734 g         8825 t
ORIGIN      
        1 tgtttaccca acatgcgctc cgcactacac tatgtgggca tttttttaag gggtggtaat
       61 gaacgattat aaaatgacgc caggtgagag gcgcgcgacc tggggtttag ggaccgtatt
      121 ctcgttgcgc atgctgggca tgtttatggt tctgccggtt ctgaccacgt acggcatggc
      181 tctgcaaggt gccagcgaag cattaatcgg tattgccatt ggtatttatg gtctgactca
      241 ggccgttttt cagattccgt ttggcctgct ttccgaccgc attggtcgca aaccattaat
      301 tgtcggtggg ctggcggtgt ttgccgccgg tagcgttatc gctgcgcttt ccgactccat
      361 ctggggaatt attctgggcc gggcgctaca aggctctgga gcaattgccg ccgccgttat
      421 ggcgctgctt tccgatctca cgcgcgaaca aaaccgcacc aaagcgatgg cgtttatcgg
      481 cgtgagcttt ggcattacct ttgccattgc gatggtgctt ggcccgatca tcactcacaa
      541 acttgggctg cacgcgctgt tctggatgat cgctattctg gcaacaaccg gcattgcgtt
      601 gaccatttgg gttgtgccca acagtagcac tcacgtactt aatcgtgagt ccggaatggt
      661 gaaaggcagt ttcagtaaag tgctggcgga accgcggctg ctgaaactca actttggcat
      721 tatgtgtctg cacatgcttc tgatgtctac gtttgttgcc ctgcccggac agctagctga
      781 tgcggggttc ccggcggctg aacactggaa ggtctatctg gcgacgatgt taatcgcctt
      841 tggctcgatc gtgcctttca ttatctacgc tgaagttaag cgcaaaatga agcaagtctt
      901 tgtcttctgc gtcgggttga tcgtggttgc ggaaattgtg ttgtggaacg cacaaacgca
      961 gttctggcaa ctggtggtcg gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc
     1021 ccttctgccc tcacttatca gtaaagagtc gccagcaggt tacaaaggta cggcgatggg
     1081 tgtctactcc accagccagt ttcttggcgt ggcgattggc ggttcgctgg gcggctggat
     1141 tgacggcatg tttgacggtc agggggtatt tctcgctggc gcaatgctgg ccgcagtgtg
     1201 gctggcagtc gccagtacca tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat
     1261 cccggcggac attgccgcaa acgaagcgtt aaaagtgcgt ttgctggaaa ctgaaggcgt
     1321 caaagaagtg ttgattgcag aagaagagca ttcagcttat gtgaaaatcg acagcaaagt
     1381 gacaaatcgc tttgaggttg aacaggcaat tcgtcaggca taaaaaacgc ggcgaattgc
     1441 ctgctgcgct acgcttacca ggcctacaaa gttcttgagt tacaaaccgt tgtaggccga
     1501 atcaggcgtt catgccgcat ctgacatgaa caaaacgcac attgtcgcga ttaatcgcgg
     1561 aagtttttga actggaacgg ctggccgaga tcgccaccac gtaccatcgc catgacagcc
     1621 tgcaaatcat cacgagattt gccagtcaca cggatctcat cgccctgaat ttgcgcctgc
     1681 actttcagtt tgctgtcttt aatcatcttg acgattttct tctgggtcgc gctctcaatg
     1741 ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat attttccggt
     1801 acatccagtg aactgccttc aatgccgcgc ttcagcagct tggcacgcag aatatccagc
     1861 aactgattga cctggaaatc ggactcgctc aacactttga tggttttgct ggcgtcgttc
     1921 agctcaaatg aggcttcaac attacggaag tcaaaacggg actccacttc gcggctcgcg
     1981 ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc gaaagatggc
     2041 atcttttcct ctcccttcat ttttgatgcg aagcataata cccgcaaagt taaataaccg
     2101 acagtcctgc accaggacgt atcccttaat tgtgcatagt cacaacagca tgcacaatta
     2161 agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac
     2221 cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta aacagggtca
     2281 cgaagttcag ggctggctgc gcgtaccgca accttattgt agtgtgaatc tggttgagac
     2341 tgatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc ttgccaccag
     2401 cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc
     2461 gtccacactg cctgtaacca cgccaatact gttaattcac aacggcatgg gcaccatcga
     2521 agagttgcaa aacattcagc agccattact gatgggcacc accacccatg ccgcccgccg
     2581 cgacggcaat gtcattattc atgtggcaaa cggaatcacg catattggtc cggcacggca
     2641 acaggacggc gattacagct atctggcgga tattttgcaa accgtgttgc ctgacgttgc
     2701 ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gccgtcaact gtgtgattaa
     2761 tccactgacc gccatctgga attgcccgaa tggtgaatta cgtcatcatc cgcaagaaat
     2821 tatgcagata tgcgaagaag tcgcggcagt gatcgagcgc gaagggcatc atacttcagc
     2881 agaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcggaaa atatctcgtc
     2941 gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca atggttttct
     3001 cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc tgtttgaaat
     3061 ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc gcccctggta
     3121 gtgaagagac tgaagccgtc accactatcg atctgctggt tcgcggcggt atcaaagtca
     3181 ccactgccag cgtcgccagc gatggcaacc tggccattac ctgctcgcgc ggcgtgaagc
     3241 tgctggcgga tgcgccgctg gtcgaggtgg ctgatggcga atatgacgtg atcgtactgc
     3301 ctggtggcat taaaggcgca gagtgttttc gcgatagtac cctgctggtt gaaaccgtta
     3361 aacagttcca ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc
     3421 tggtaccgca cgatatcttc ccgattggca atatgaccgg cttcccgacg ctgaaagaca
     3481 aaattcccgc cgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc
     3541 tgaccagcca ggggccgggt acggctattg attttggtct gaaaattatc gacctgctgg
     3601 ttgggcgcga gaaagcccat gaagtggcat cacaactggt gatggcggca gggatttata
     3661 attattacga gtagtgtcgg atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa
     3721 ccacaaatta tgggcgatac accttcacat tgttaaagcc ctgctcgcgc agatagagcg
     3781 cctgcagacg gctcatcacc ccgcgctcac accacagtag ccaggttctg ttctggtcga
     3841 gatcgccaaa tttggtgctc agtttataga acggcagaga aaccacgtcg ataccttcaa
     3901 ccttcagcgg cttatcttcc tgctcatcga cagaacggat atcgaggatc acgtcgttcg
     3961 ggccaaagcc attgacggtt tccacttcca ccacttcctg ctcggtctgc tgggcgattt
     4021 cgcggatatc aacgttattc gcttcctcaa ccactttatc gagaatactg aagtcgaact
     4081 tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac cgtcgggctt ttggagatca
     4141 cgccacaata ttctggcatc gtgcgagcaa agtcttcagt gccaatctgg cgggccaggt
     4201 tgatgatgtg ctctttgtcg taagaaatca gcggacgcag gatcagcgta tcagagacgt
     4261 tatcaatcag gcgcaggttg gtcagcgtct gactggacac ctggccgagc gcttcgccgg
     4321 tgaccagcgc ctgtacgccg taacgttcag ccactttaga cgcggcacgc accatcatac
     4381 gtttgaggat aacgcccatc tgaccgtcgt cgattttctc gagaatttcc ccgacgaccg
     4441 gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct gccaaagcgg ttccacagat
     4501 aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc gccgaggtta aagaagcagt
     4561 aatgcacgcg gcagccgcga cgcatcaaca tataactgga aacaccggag tcgaaaccac
     4621 cggaaatgag cgacagcaca tcttcctggg tgccaatcgg gaaaccgcca ataccttcgt
     4681 agcggccttt aatcagcaga agacgatcgt cttccacttc cagatggaca gttacatccg
     4741 ggttagtcag cttcacgcgc gcggattcaa tatgctgatt taaaccgccg ccgacgtaac
     4801 gttccacatc aatcgagcta aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg
     4861 ttttgccttc cagctgatcg cgatattgaa ccaacgcttt ctcgaaaata tcgtgcatgt
     4921 cggtaaacgg cacgtcttcg acttcgagaa tatggtggat acccggaata cgggtcaggg
     4981 cgtcgcgaat agccagacgc tgtttttcat cttttgcgcg aacttcgatg ttatcccagt
     5041 ggcggacgac agcgagcgtc tcatcatagt gctttaaaac gttacgaatg ttcccggtaa
     5101 ggatttttat aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc gggaacaatt
     5161 taatgataaa cttcatggcg gcaatggttc gttggcaagc cttaagcgac ttgtataggg
     5221 aaaaatacag cagcccacac ctgcggctgc atccaggcgc ggaagtatac cactaacatc
     5281 gctttgctgc gcacatcacc ttaccattgc gcgttatttg ctatttgccc tgagtccgtt
     5341 accatgacgg ggcgaaaaat attgagagtc agacattcat tatgccgaag aaaaatgagg
     5401 cgcccgccag ctttgaaaag gcgctgagcg agctggaaca gattgtaacc cgtctggaaa
     5461 gtggcgacct gccgctggaa gaggcgctga acgagttcga acgcggcgtg cagctggcac
     5521 gtcaggggca ggccaaatta caacaagccg aacagcgcgt acaaattctg ctgtctgaca
     5581 atgaagacgc ctctctaccc ccttttacac cggacaatga gtaatggact ttccgcagca
     5641 acttgaagcc tgcgttaagc aggccaacca ggcgctgagc cgttttatcg ccccactgcc
     5701 ctttcagaac actcccgtgg tcgaaaccat gcagtatggc gcattattag gtggtaagcg
     5761 cctgcgacct tttctggttt atgccaccgg tcatatgttc ggcgttagca caaacacgct
     5821 ggacgcaccc gctgccgccg ttgagtgtat ccacgcttac tcattaattc atgatgattt
     5881 accggcgatg gatgatgacg atctgcgtcg cggtttgccg acctgccatg tgaagtttgg
     5941 cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg ctggcgttct cgattttaag
     6001 cgatgccgat atgccggaag tgtcggaccg cgacagaatt tcgatgattt cagaactggc
     6061 aagcgccagc ggtattgccg gaatgtgcgg tggtcaggca ttagatttag atgcggaagg
     6121 caaacacgta cctctggacg cactcgagcg tattcatcgt cataaaaccg gcgcattgat
     6181 tcgcgccgcc gttcgccttg gtgcattaag cgccggagat aaagggcgtc gtgctctgcc
     6241 agtactcgac aagtacgcag agagcatcgg ccttgccttc caggttcagg atgacatcct
     6301 ggatgtggtg ggagatactg caacgttggg aaaacgccag ggtgccgacc agcaacttgg
     6361 taaaagtacc taccctgcac ttctgggtct tgagcaagcc cggaagaaag cccgggatct
     6421 gatcgacgat gcccgccagt cgctgaaaca actggctgaa caatcactcg atacctcggc
     6481 actggaagcg ctagcggact acatcatcca gcgtaataaa taaacaataa gtattaatag
     6541 gcccctgatg agttttgata ttgccaaata cccgaccctg gcactggtcg actccaccca
     6601 ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac tgcgccgcta
     6661 tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg gcacggtcga
     6721 actgaccgtg gcgctgcact atgtctacaa caccccgttt gaccaattga tttgggatgt
     6781 ggggcatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat
     6841 ccgtcagaaa ggcggtctgc atccgttccc gtggcgcggc gaaagcgaat atgacgtatt
     6901 aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg ttactgccga
     6961 gaaagaaggc aaaaatcgcc gcaccgtctg tgtcattggc gatggcgcga ttaccgctgg
     7021 catggcgttt gaagcgatga atcacgcggg cgatatccgt cctgatatgc tggtggtcct
     7081 caacgacaat gaaatgtcga tttccgaaaa tgtcggcgcg ctcaataacc atctggcaca
     7141 gctgctttcc ggtaagcttt actcttcgct gcgcgaaggc gggaaaaaag ttttctctgg
     7201 cgttccgcca attaaagagc tgctcaaacg taccgaagaa catattaaag gcatggtagt
     7261 gcctggcacg ttgtttgaag agctgggctt taactacatc ggcccggttg acggtcacga
     7321 tgtgctgggg cttatcacca cgctgaagaa catgcgcgac ctgaaaggcc cgcagttcct
     7381 gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc ccatcacttt
     7441 ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta gcggcggttt
     7501 gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga aagacaacaa
     7561 gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt tttcacgtaa
     7621 attcccggat cgttacttcg acgtggcaat cgccgagcaa cacgcggtga cctttgccgc
     7681 cggtctggcg attggtgggt acaaacccat tgtcgcgatt tactccactt tcctgcaacg
     7741 cgcctatgat caggtgctgc atgacgtggc gattcaaaag ctcccggtcc tgttcgccat
     7801 cgaccgcgcg ggcattgttg gtgctgacgg tcaaacccat cagggcgctt ttgacctctc
     7861 ttacctgcgc tgcataccgg aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg
     7921 ccagatgctc tataccggct atcactataa cgacggcccg tccgcggtgc gctacccgcg
     7981 cggtaacgcg gttggcgtgg aactgacgcc gctggaaaaa ctgccaattg gcaaaggcat
     8041 tgtgaagcgt cgtggcgaga aactggcgat ccttaacttt ggtacgctga tgccagacgc
     8101 ggcgaaagtc gctgaatcgc tgaacgctac gctggtcgat atgcgttttg tgaaaccgct
     8161 tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca ccgtagaaga
     8221 aaacgccatt atgggcggcg caggcagcgg cgtgaacgaa gtgctaatgg cccatcgtaa
     8281 accagtaccc gtgctgaaca ttggcctgcc tgacttcttt attccacaag gaactcagga
     8341 agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg
     8401 gctggcataa tccctactcc actcctgcta tgcttaagaa attattcatg gcaggagtgg
     8461 cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg
     8521 ctgtatgacc tttggcgagc cagatcgtgg taatcacgca tggacactgc cggaagagag
     8581 tagccgtccc atcattaaac gcgcgctgga aggcggcata aatttctttg ataccgccaa
     8641 tagctattcc gatggcagca gcgaagagat cgttggtcgc gcactgcggg atttcgcccg
     8701 tcgtgaagac gtggtcgttg cgacgaaagt gttccatcgc gttggtgatt taccggaagg
     8761 attatcccgt gcacaaattt tgcgctctat cgacgacagc ctgcgccgtc tcggcatgga
     8821 ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg aagagacgct
     8881 ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat
     8941 gcacgcttcg cagtttgctc aggccctgaa gctacaaaaa cagcacggct gggcgcagtt
     9001 tatcagtatg caggatcact acaatctgat ttatcgcgaa gaagagcgcg agatgctgcc
     9061 tctgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc gggggcgact
     9121 gacgcgtccg tggggagaaa ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct
     9181 ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacgg gcgtcagtga
     9241 agaactcggt gcaacacgag cacaagttgc gctggcctgg ttattgagta aaccgggcat
     9301 tgccgcaccg attatcggta catcgcggga agaacagctt gatgagctgc tgaacgcggt
     9361 ggatatcact ttgaagccag aacagattgc cgaactggaa acgccgtata aaccgcatgc
     9421 ggtcgtagga tttaaataag agtccaggcc tgatgagacg tgacaagcgt cacatcaggc
     9481 atcggtgcac aactacgaca gaatacccag cggccagtga tgaccgataa agtacaggat
     9541 gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg atccccatgc cgccatgtac
     9601 attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac ggaaaatcac
     9661 aaacccggcg gcaacccact gccagtcatt ggtcggcagc gccatgagcg tgatccacat
     9721 accaataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat ctttcgccgt
     9781 ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg agtagagctg
     9841 ccagggtaaa aaggtcatca gataccagaa cggaatcgct gccagcgatc ccatcgtgcc
     9901 aggaacgatt gggcttaatc cacttccgaa tccgacagca agcagatgcc acggattact
     9961 catcttcagg cgacttttcg cgacatcttt atggcgtggc aaaatggtca tatcctttcc
    10021 agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata tcggcggtca
    10081 tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag agcaacatcc agcgcgccac
    10141 ggttcagttc cggcaccgtg aaacacaact cgtaatcttc accgccagag agcgcccagc
    10201 gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc agcaatgcta
    10261 ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg gaaatcagac
    10321 cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac
    10381 gcggcgatgg acggagatga cgtttgatca agtagtcagc atctttagca tcggcaacct
    10441 gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta ccggtcacat
    10501 agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc
    10561 cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca atgagttgca
    10621 tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt
    10681 ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc atcgctgcca
    10741 gatcgcttag gttcaccgcc agtgctttat aagccagatc agcaggatcg atatcaggga
    10801 ggaaatggtt acccgccacc agcgtatcag tgctgatcgc cagggtctgt ttctcgggga
    10861 tattgagaag tgcgcaatcg tcgccgatgc ccagttcgac atcaagacga gaacttctta
    10921 cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt tatgcctcag
    10981 caggtaaaaa agaaaaggcc gacgatcatg gaacggtctt ccgtgaatct accggcctgg
    11041 atatcacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa
    11101 cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt taatggccac
    11161 tttgtatggc acatcgctac gtttagacag ttcgtacagc gcaatgcgca gtactgcttt
    11221 ttctacctgt cccagttctt ccagcaggcg ggacaggtat ggcttcatca gtccgtcgag
    11281 gtatgcggta ttagtcgcca ccccggccag cagctcacgg aagtacagga cgtcaacgtc
    11341 ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt tctgggacaa
    11401 ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag cagcaggttt
    11461 cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta atcatttcaa
    11521 gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca cgttcgatcg
    11581 cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg
    11641 caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg gcagtgccac
    11701 cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta gccagtgcac
    11761 ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg ttttcatctt
    11821 ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa
    11881 agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca acgttagctt
    11941 caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg gcggatttta
    12001 gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc cactaaatgc
    12061 aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg ggcgtcggct
    12121 aatttctcaa gccctggcag cgtgcataat ccgcgggcgt cgctgcctaa tagtttaggt
    12181 gcgatataga caatcagctc atcgactaaa cccgcctgca gcaatgcgcc agcgagcgtt
    12241 ggccccgctt ccacccagat gctgttaatt tgctgtttac ccagttgcat catcagtaca
    12301 accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt ttccggccac
    12361 tcactagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg cacaatgcga
    12421 tgttccggcg tcacgcgatt ttggctatca atcacaatac gtactggctg gcggagattt
    12481 tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac tgttaaggct
    12541 ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg catgactttg tgcgcgctgt
    12601 cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc
    12661 gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc
    12721 atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag cccgtggctg
    12781 acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg cgggttagga
    12841 tcctgcatcg ctgcgaccac gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt
    12901 ggcgtacggc catgatggct acagggttcg agtgtcacat acgccgttgc gcctttggct
    12961 ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc tcgttggtgg
    13021 taaccttcac cgacaatttc gccatctttg acaatgacgc acccgacatt cgggttgggg
    13081 tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta atactcgtcc
    13141 tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa
    13201 ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt caattgctcc
    13261 atcaccagat tgccaatcat cttgctcggt acttcgcgct caccggtggc gcgcagctgc
    13321 gatttaatat gattgattgc catttcaacg tcatcggaac tcaccggacg tttttccagc
    13381 gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac gtcgttgctt
    13441 tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg ttcattacac
    13501 accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg agagtcaatt
    13561 accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga ccaggtggtt
    13621 aacggaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc ttttgagata
    13681 aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg acgagaaagg
    13741 gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg ttcagcctta
    13801 gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc
    13861 aggagatgac gcaactcaat caagaaacag tcaaaatcac tcagcaaaac aggctgaatg
    13921 caaaatccag cagtggggtt taccttctgc ctggagcgaa aacaccggca agactggaaa
    13981 gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgataca gatggcacca
    14041 cccttacact acgtattcag ggtgagtcga atgatccctt gcccgcattc agcggaactg
    14101 ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat gtgcagaatc
    14161 aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt ccgttacaat
    14221 taaaggggat ttctgtggat cagttaggct ttgtacgtat ccacgacatt caacctgtta
    14281 tgcagtaaac gtatttcggg acgattttgt gcgtcccgca acatctttcc ccgtcatttt
    14341 gttactctgc ttacatcacc tggattgata gtaaaagttt gcaacaaggg cgaaagtcag
    14401 tacaatcccc gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa atgatagaac
    14461 tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct ctgtatacga
    14521 aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc aaacagtggc
    14581 atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc ttcgtctttt
    14641 actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg gcaccagagc
    14701 gttaacgttg tcggaagcta tcacacccgt ttcggaccgc agatccgcaa cgatacctac
    14761 cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta tgcggatgcg
    14821 ccggtattct tcggcggtaa ctccgatgct aaaggtatct ggaaccacgg ttctccgctg
    14881 tttatggaaa tcgaaccacg tttctccatc gacaagctga ccaatactga ccttagcttc
    14941 ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg tcgtaataaa
    15001 gatggtcgcc agagcacctg gtacatgggt ctgggtaccg acatcgatac tggtctgccg
    15061 atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactatga cgcagcgaac
    15121 gaaaacgagt gggacggtta ccgtttcaaa attaaatact ttgtgccgat taccgatctg
    15181 tggggcggtc agctgagcta catcggcttc accaacttcg actggggttc cgatttaggg
    15241 gatgacagcg gtaacgcaat caacggtatt aagacccgta ctaataactc tatcgcttcc
    15301 agccatattc tggctctgaa ctacgatcac tggcactact ctgtcgtagc tcgttactgg
    15361 cacgacggtg gtcagtggaa cgacgatgca gaactgaact tcggcaacgg caacttcaac
    15421 gttcgctcta ccggctgggg tggttacctg gtagtaggtt acaacttctg attatgaaaa
    15481 tgccgggatt tattcccggc atttctgatt gttaacaacg tgtgctttgt tcatgccgga
    15541 tgcggcgtaa acgctttgtc cggcccacaa aagcacgtaa attcaatata ttgctgaaat
    15601 aacgtaggcc tgataagcgt agcgcatcag gcaattttac gttttaatca cttcttcttg
    15661 ttcatcatcg ctttcaggtc agcgaatggg ttgtacttcg cttcaccgac atctttctgc
    15721 gcatcttccc ctgcgataac ggtcgtacca tactgatccg cttcggtata tttcgaatgc
    15781 tcatgatcgt ggcaatagag acaaagcaat tcccagttac tgccatcttc cgggttattg
    15841 gtatggtcgt gatcaatgtg gtgaacggta agttcacgca ggttggaata aacaaactcg
    15901 cgggaacagc gaccgcagac ccacggatag atttttaatg ctttttcgcg atagccgctt
    15961 tctaaccgcg catagttttt tgggatgata gccatgaaaa gttacctgga tgaaatgtag
    16021 ggttacgcgc agtttcccaa gatgcgggga ttttcgcaat ctccatacat caaaaaaaat
    16081 cccgatcttc tgaccgggat tttcaacatc aacgggaact tgattacggc agaattgacg
    16141 gctgatccgc cccttctttt tccactttct gctgcaacat gtgttcgcgc ttcataccca
    16201 gtttcagagc caacgcagat gctacgtaga tggaagatgc agtgccgatg gaaacaccga
    16261 taagcatggt cagcgagaag ccttccagta ccggaccacc gaagagatac agcatcagga
    16321 taaccatcaa ggtagtaccg gatgtgatca aggtacggtg cagcgtctgg gtcaaggaca
    16381 cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg gaagttttca cgaattcggt
    16441 ccgataccac gatactgtcg ttaagcgagt aaccgataac cgacatcaac gatgccacaa
    16501 tggtcaggtc aatctcgata tggaataacg acaaaatacc cagcgtaatg ataacgtcgt
    16561 gcgccagcgc aataaccacc cctgccgcca gtcgccactc aaagcggaaa cctacgtaca
    16621 cgaggataga cagcagcgct gccatcaacg ccatcgcacc ggtttgcgca aggtctgccc
    16681 ccacgctcgg accgacgaac tcaatacgct tcactgctgc attctgattg gtggattcgt
    16741 taatcacctt cagaacctgg ctgcccagca cctgaccgcc ggtttcgcct tcagcaggcg
    16801 gcatacggac catgatgtcg tggctgctac caaagttttg cagcattggc tcttcaaaac
    16861 cggctttttg caatgcatca cgcattacgt caatttcagc cggtttttcg agcgtaattt
    16921 cgataaccgt accaccggtg aaatccagcc cccagttaaa gccgcgcacg cccataataa
    16981 cgatagcagc gattaacagc agaccagaga tgccgaaagc ccagtagtcc cagcgcataa
    17041 agtcatagac tttacggccg tggtttagtt gttcaacagt atattcctgt gccacatcgc
    17101 actcctcaga ttgacagctt cttgacgcgc ttgccgccat atagcaggtt tacgatggca
    17161 cgcgtaccga caatcgcggt aaacatcgac gtcgccacac cgataccggt agtaatcgcg
    17221 aaccctttaa ttgccccggt acccactgcg tacaggatga tgactttaat cagcgtggtg
    17281 atgttcgcat cgaagataga actgaatgcg ccacgataac cttcatcaat tgcctgttga
    17341 acagtacgtc cgttgctcaa ctcttcttta atacgttcgt tgatcagtac gttcgcatcg
    17401 accgctaccg caagggttaa gacgataccc gcgatacctg gcatactcag tgttgcgcct
    17461 ggcaacagcg acataatacc gacgatcaag atcaagttgg caatcagagc actggtcgca
    17521 atcagaccaa acttcttata gaagatgatc atgaacagaa tagaaaccag cagaccggca
    17581 aggcaagcct ccagcccctg ctcaatgttc tgcataccca gggtcgggcc aattgtacgt
    17641 tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac gcagcagcag tgacagctga
    17701 cgggcttcgt tcgggttgtt gatgccggtg atacggaagc tgttacccag acgagactgg
    17761 atgttggcga tgttaatcac ctcttcctgt ttcaccagaa ccgcacgacc atttgcatct
    17821 ttcttaccgc tgtctttgta ctccacaaac agggttgcca tcggtttgcc gatgttgtcc
    17881 ttggtgaagt tagacataat gttgccacca gcgctatcga gcgagatgtt aacctgcggc
    17941 tggttgtatt cgtcctggct ggaagtggag tcggtgatat ggtcaccggt cagaattacg
    18001 cgtttgtaca gcacaaccgg ctgaccttcg cgggtctgtt tcacttcaga gtcgcccggt
    18061 acgcgaccgg atgccgcagc ggcctgatca acattggtgt ttaccagacg gaattccagc
    18121 gttgccgtcg cgcccagaat ctctttcgca cgcgcagtgt cctgaatacc tggcagttca
    18181 acaacgatac ggtcagcacc ctgacgctga accaccggct ccgccacgcc aagttggttt
    18241 acacggttac gcaggatatt aatgttctgc tgcaccgcat attcacgcgc ttcactcaga
    18301 cgagcatcgc tcattaccgc acgcagctgg ttgctgccct ggctgctaat caccaggtcc
    18361 ggatggcgct tgctcagata cgcaatggct tcatcacgag ctttagcatc gcggaaagtg
    18421 atgctcaggc cgtagttgtt ttctttacga acagtggtat acgggatgcc cttttcgcgc
    18481 aggtcactgc gtaggctatc gatattttgt tcctggagtt tgccaagcgc ggtgtccata
    18541 tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa ggccgagctt catcggctca
    18601 gcatgaatag ctgccagcca gcgcggcgtt gccggggcaa ggttaagcgc cacgacgtat
    18661 ttgtcaccca taacgcccat taatgcttca cgagcgcgca actgggtgtc agtggagtcg
    18721 aagcgcgcaa gaatagcgcc ctcttccagt gccacagact tagcagttat tttttcttct
    18781 tgtaacgttt tctggacctg gatcagcgtt tgctcactgg cggcgactcc gcgcgcacca
    18841 gtgatctgaa cagccggatc ctcaccaaac aggttgggaa gcgcatacag cagaccgatg
    18901 acaatcacca cgatcagcat gacgtacttc cacaaaggat aacggtttaa cacggcaatt
    18961 cccttaggga aaaattttaa ttacagcgcc ttcatggtgc ctttcggcag gacggcagct
    19021 acgaagtcac gtttaataac tacttcagtg gtgtcattca gcgcgatagc aatgtagccg
    19081 ttttccgcta ctttggttac gcgaccaacc aggccaccgt tcgtcagaac ttcatcacct
    19141 ttggcaatgg agtccatcag ctttttgtgt tctttggtgc gcttctgctg tggacgcagg
    19201 atcatgaaat agaaaatcag accgaatacc accagcatca aaatcaaaga catcgggcta
    19261 ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa taaaaaagct cattaaattt
    19321 ccctcattat taatattaat caacgttcaa aggtggtact tctcgcccct gacgctggta
    19381 aaaatcagtt acgaagctct ctaatttacc ctcttcaata gccttgcgta aacccgccat
    19441 caaacgctgg tagtaacgaa ggttatgaat ggtgttgagt cgcgcgccta atatttcgtt
    19501 gcaacggtca agatgatgca agtaagcgcg tgaataattg cgacaggtgt agcaatcaca
    19561 ctcaggatca agtgggccag tatcgctctt atacttcgca ttgcggattt tcaccacgcc
    19621 atcggtcacg aacaaatgac catttcgggc gttgcgggtt ggcattacgc agtcaaacat
    19681 atcgatacca cgacgtacgc cttcaaccag gtcttctggt ttaccaacgc ccatcaggta
    19741 acgcggtttg tctgccggaa tttgcgggca tacatgctcc agaatgcggt gcatatctgc
    19801 tttcggctca cccacagcca gaccgccgac agcgtagcca tcaaaaccga tatctaccag
    19861 acctttaaca gaaatatcac gtaaatcttc gtaaacgctg ccctgaatga taccaaacag
    19921 cgcatttttg tttccgagac tgtcaaaacg ctcacggcta cgcttcgccc aacgcagaga
    19981 catctccatg gagcgttttg cgtaatccca gtcagcagga tacggcgtac actcatcaaa
    20041 gatcatgacg atatccgaac caagatcgta ctgaatctcc attgattttt caggatcgag
    20101 gaaaatcgga tcgccgttga tcgggttacg gaagtgcacg ccctgttcgg tgattttacg
    20161 aatatcgcca aggctgaaga cctggaagcc gccggagtcg gtgaggatcg gccccttcca
    20221 ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt tcctggcccg ggcgcagcca
    20281 caggtggaag gtgttgccga ggataatttg cgcgccagtg gcttcaactt cttccggcgt
    20341 catccctttt acggtgccgt aggtgccaac aggcataaaa caaggcgttt ccactacgcc
    20401 acgatcaaag accaggcggc cacggcgtgc gcgaccgtcg gtggtgtcca gttcaaattt
    20461 cattttttct ccactacgtc agaaaaacag tccaacgttt taaaccagcg ccacggaatt
    20521 actccccgac gcgctcatta attgcctgcg gattgtacgt gataaacatc gcatcaccgt
    20581 aactaaaaaa gcgatatttc tcttctaccg ctgctttata ggcgttcatg gtgtgttgat
    20641 aaccggcaaa ggccgaaacc agcataatca gcgtcgattc cggcaggtgg aagtttgtca
    20701 ccagcgcatc gaccactttg tactggaagc ccggatagat aaagatttgg gtatcgtcaa
    20761 agaacggttc gataagatcg tttttcgccg cctgagccgc gctttccagc gaacgtactg
    20821 aggtggtgcc aaccgcaatc acccggttac cgcgcgcttt cgccgccagt accgcgtcta
    20881 ccacatcctg cggcacttca gcgtattccg agtgcatgat gtgatcttca atggtgtcga
    20941 cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt cacaaacgcc atctccacgc
    21001 ctttggcgcg taatttttcc agcaaaggtt cgtcaaaatg cagacctgcg gtcggggctg
    21061 caaccgcgcc cggtttttcg ctataaacgg tttgataaag ttcgcggtca gcgtcttcgt
    21121 ccggacggtc gatatacggc ggcagcggca tatggccgat gctgttgaga atatccagca
    21181 ccgagcgttc atcattaaat tcgacttcaa acagtgcgcc gtggcgcgcg gtcattgttg
    21241 cgttaatact ttcgtcatcg cccagcagca gttctgcgcc aggttttggc gctttcgagg
    21301 cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg ttcaaccagc acttcaatct
    21361 tgccgccgct ggctttacgc ccaaacaggc gcgccgggat cacgcgggta ttattaaaaa
    21421 ccagaagatc gccggggttg agcttatcaa gtaaatcggt gaaagtaccg tgcgtcagcg
    21481 cgcccgtcgg cccgtccagc gacagtaaac gacagctact gcgttcaggc atgggatagt
    21541 gggcaatcag ggattcgggc aattcaaagg agaaatcggt aacgcgcatg acactgactc
    21601 agactaaaat aagaggcggg tagtctagtg ccggggcgct ctccctgcaa cctttacccc
    21661 cttttggata gaatataatc gatgaatttt ttagctcacc tgcatttagc ccatctcgcg
    21721 gaaagctcgc tttccggcaa tttactggct gatttcgtac gcggaaatcc tgaagaaagt
    21781 tttccgcccg acgtcgtggc tggcattcat atgcatcgac gtatcgacgt attaaccgac
    21841 aatctgccgg aagtccgcga agcacgggaa tggtttcgta gtgaaactcg ccgcgttgcg
    21901 cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc gcagctgtcg
    21961 ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat gacgattttg
    22021 ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga gcagtggctg
    22081 gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc aagccgccgc
    22141 ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta tgacgccctc
    22201 gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca caaggcgtta
    22261 taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga atcccgtcag
    22321 tcagttgccc atgcctttct ttgcattgcc attgcacggc attgagcaac ggtgacgcgg
    22381 gtagcacgac ttcacaagct tcgccacggt tgattgccac cagtacacgc tgctgattca
    22441 gcacgcggac aaataccacc acgttatctt ccgcatacag cacctgacag ccgccatgac
    22501 gtagcgcctg acttttctta cgcagcgcaa tcattcgctg gtacagcgcg aataacgccg
    22561 tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg tttttgccat
    22621 ccagtcctac ttcatcaccg taataaatgc acggtacacc aggccaggtg aacagccaga
    22681 ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat cgcgcagtat
    22741 cgtggctgtc gagctgatta aacatacgta attgttgttg atgagaaagc cctgcgcggt
    22801 aattatccat ccaggccata caggtttggg catcaatttg ctgcggatcg taagagatat
    22861 cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc atggcggcat
    22921 cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg acaatatacg
    22981 cttccggctg ggtttctttc gccgcttcgg tgctcccagc aacgtgctgc atattattgc
    23041 gcgccccacc cgcctcccct agcatatgca ccacatccag ccgccagccg tccatactcc
    23101 acggcgcttt cagccaatgg cggacaatac tgtcttcccc gcgataaatt tcattcacca
    23161 gactttccga ctgataatcc agcttcggca agctggcata gccaagccag tcgagcgccg
    23221 tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagggttg tgacaagctc
    23281 cccccgtgcc acgattgtgc ctgtcaaacc aggcatggga atcgccactg tggttaaaca
    23341 cgccatccag ccccagccgc attcccagct gctgcgtatt gtggcgtaag cgcagcaacg
    23401 ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg tatttatgta
    23461 cgctgggagc tttaaacacc ggattgagat acagcgccgt cacgccaagc tttttcagat
    23521 acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat ccgcccgcct
    23581 gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct gcatgatggt
    23641 aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga tcagggaaga
    23701 tctgataaaa aatctgatcc gcagcccatt gtgggccgat atccggtaca tcgacggcaa
    23761 actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac caacgctggc
    23821 gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg gagagatcaa
    23881 tcgccgcacg ccatgcggtg acgccaggct gctgctgact gcgatgctta tgcatcgata
    23941 ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt gggtcttcgc
    24001 ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat gggggcaccg
    24061 gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat caccttgcca
    24121 tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg acgggggcgg
    24181 ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag aacgcaagga
    24241 ttactggttt tcagccagct gacgatcgtg gcgacgctta aacatccagc caatcaacag
    24301 cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt gataaccaat
    24361 caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta ccccacccgg
    24421 cactttaaat ttcagcgcct taacttcttc tggcggcaaa cagcgacgga aggcaatttg
    24481 cgacagcagg atcataatcc acacccacac cgtggcgaag gttgccagcg aagcgatcac
    24541 caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca gcgcggtagt
    24601 catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga aaattttcgg
    24661 cgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaaga cgtcactgtt
    24721 aattgccgat agcgaagcgg tcagcacaac aaagttaaga atgctggcgg caaaggtaat
    24781 gcccatatgc tggaacgtca gcacgaacgg gctaccggca gtgccaacct gattccacgg
    24841 gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac gcatcggcac
    24901 ggagttaatc gcacgcggta tcgatttctc aggatctttc gcttcaccgg cggtaatccc
    24961 gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatca ccatcccaag
    25021 ccagccgtta ctgaagaagc cgccgttgct ccacagatta tgaataccgg tcggttgccc
    25081 gccgttgcca atcccccaga tgatgatgcc gaaaccagcg acaatcatga tgatgatggt
    25141 ggcgacttta aagaacgaga accagaattc cagctccccg aagaccttca cgctcatcag
    25201 gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg gcaccgtcgg
    25261 gaaccagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga caataaggat
    25321 ttcaaagcag taggtccagc cggtaatgta acctgccagc gggccgaggt tttcctgcgc
    25381 ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca gcgcacgcat
    25441 aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg gaccggccat
    25501 tttgatggcg tctgccgaac cgtaaaacag cccggtacca attgctgaac ccagtgccat
    25561 aaagcgtatg tggcgggtac ttagcccacg ctttagcttg ttcttacttt ccatcaattc
    25621 ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt aaaacaaatg
    25681 ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat cccagataat
    25741 ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt cggccagcgg
    25801 taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga tcccgtcgag
    25861 aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg aattatgcca
    25921 ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa tagcggtcag
    25981 caccggtacg gagatctgga tcagctggct taagccgagg ttagaaacca ccatcgagaa
    26041 gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg caaagaattc
    26101 tgcacaagca caggtcaggc caactgccgt taccaagcag gcgatgaaga ttaacgccgc
    26161 cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca gaatagcagc
    26221 gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacgga acagcgccag
    26281 gtacagcaga gtcagaccaa cacccgccat caggccagcc cagacggtat aacgggtcag
    26341 cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga taccaaacac
    26401 cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag aaaacgcagc
    26461 gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa tcgcggcaac
    26521 agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca cggtatccag
    26581 cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca ggctgtagat
    26641 aaacagcggc agcgcggaat caccagtcag cggcgcaatc cccacttcaa aggaaacggt
    26701 agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg ttgccagcag
    26761 cacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc caacttttgc
    26821 cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc cgaatgccgc
    26881 agtccagacg tgttcgccag cctgcaagcc gaccattgga gggaaaataa tgttacctgc
    26941 gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg atcttaattg
    27001 atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt tttgtgccaa
    27061 tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac tcccaaccag
    27121 gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg acagcacctg
    27181 aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa accagaggta
    27241 tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa agacatggct
    27301 aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat tttgactgca
    27361 aagaaaataa gccagcatcg actggcttat ggcaataaaa gatgacaaag gcggattaat
    27421 cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt ccttttccta
    27481 ctgtactctc aatattcagg cgactttcgt gatgattcac agcatgtttc acgatcgcta
    27541 accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga taaaaacgct
    27601 cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca acgctaaatt
    27661 cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttct ggagtatgat
    27721 tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg ttgccagaca
    27781 ctttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc agagtctgag
    27841 cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg agcaattgcg
    27901 tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct tccatccgct
    27961 gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc agcggctgct
    28021 catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac tcatggctca
    28081 cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg acatcacgcg
    28141 ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga tgccgcccgg
    28201 tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat tgcgtaaact
    28261 ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag cgcaaaccaa
    28321 gaatttgttg cgccagaccg ttacaccaga aaataccgct ctcttccgtg gtcagcacca
    28381 ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg cccagttcac
    28441 ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc ggttcccagc
    28501 taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat gaaaggcgca
    28561 ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa aaccagggca
    28621 ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc agctccagca
    28681 ccagcctttt ccacgacagc cgttccagca cgtaagatac tcccgttaag aaatcataag
    28741 ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga tatcctgtac
    28801 cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta cgcaggcgac
    28861 gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag acgtggttta
    28921 acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc agcagtttaa
    28981 attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga gatgtcgggt
    29041 cgagacttaa tccctgcatc tcaatcacct cttccaccgc cattggcgaa atacggcgca
    29101 ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa cggcttggtg atatagtcat
    29161 ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg gtcaacatca
    29221 ccactggaat atcccgggtc atcgactcgc gcttaaggtg tttgatgaac tggataccgg
    29281 agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca ttcagttgat
    29341 tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt tcgagcacga
    29401 agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt ctcgccatga
    29461 tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat gcgtcagatt
    29521 tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt tccgtggcgc
    29581 agaaaaaagc aaatggcaca tctgtttggg tataatcgcg cccatgcttt ttcgccaggg
    29641 aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact tctacagtaa
    29701 aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag cacaaaccca
    29761 tcaggtggat gcgattattg ttgccggtga tgttttcgat accggctcgc cgcccagtta
    29821 cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct gtcatctggt
    29881 ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg atatcatggc
    29941 gttcctcaat actaccgtgg tcgccagcgc cggacatgcg ccgcaaatct tgcctcgtcg
    30001 cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgtccgc gtgacattat
    30061 taccagccag gcggggctta acggtattga aaaacagcag catttactgg cagcgattac
    30121 cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc agcctctgcc
    30181 catcatcgcc acgggacatt taacgaccgt gggggccagt aaaagtgacg ccgtgcgtga
    30241 catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag ccgactacat
    30301 cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg ttcgctattg
    30361 cggctccccc attccactga gttttgatga atgcggtaag agtaaatatg tccatctggt
    30421 gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg taacgcaacc
    30481 catggcagtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac agtggcgcga
    30541 tgtatcgcag gagccacctg tctggctgga tatcgaaatc actactgatg agtatctgca
    30601 tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag tattgctggt
    30661 acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg aaaccctcag
    30721 cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac tggatgaatc
    30781 gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cataccctcg ccggagaaca
    30841 cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa aggcgaatgg
    30901 aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgctat taccggccca
    30961 acaggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta tcacgaaact
    31021 ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga taccgccgaa
    31081 tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt accgtgcatt ctggagccag
    31141 aatcgggcgc gtaaccaacc cgacggtaat ttgcaggtgc cacgcgtaga gctggcgcgc
    31201 tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact gacagcgacg
    31261 ttaaccgggc tggattacgg gcgcttcact cgttcgatgc tgctttcgca ggggcaattt
    31321 gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga gttaaccggc
    31381 actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc ggcccgcaca
    31441 gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc ggaacaagtg
    31501 caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt aattaccgcg
    31561 cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt gcagcaagaa
    31621 gccagccgcc gtcagcaggc cttgcaacag gcgttagccg aagaagaaaa agcgcaacct
    31681 caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca ctgggaacgc
    31741 atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga agtaaatact
    31801 cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc gaagcagtca
    31861 gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca cgaccgcttc
    31921 cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca aaccagcgat
    31981 cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa acttaatgcg
    32041 cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctagcgccct ggcgcaacat
    32101 tccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat tgttccccaa
    32161 caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga acagacgcaa
    32221 cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca gcaacttgcc
    32281 gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc tcaacgtgca
    32341 cagttacagg cgggtcaacc ttgtccactt tgtggttcca ccagccaccc ggcggtcgag
    32401 gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactgacgct ggaaaacgaa
    32461 gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc attaacgaag
    32521 cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca agcacttact
    32581 caacaatggc aagccgtcac gaccagcctc aatatcacct tgcagccaca ggacgatatt
    32641 caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact cagccaacga
    32701 catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta tcaacagcaa
    32761 attgaacaac gccagcagca gctcttaacg gcattgacgg gttatgcact gacattgcca
    32821 caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca gagctggcag
    32881 caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac gccgattctg
    32941 gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc gctggataac
    33001 tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac gttacagcaa
    33061 caggatgttc tggcggcgca aagtctgcaa aaagcccagg cgcagtttga caccgcgcta
    33121 caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga tgaacaaaca
    33181 ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca ggcgcaaacg
    33241 ctggtcacac agacagcaga aacgctggca cagcatcaac aacaccgacc tgacgggttg
    33301 gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca ccaaaagttg
    33361 cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga tgcagataac
    33421 cgtcagcaac aacaaacctt actgcagcaa attgctcaaa tgacgcagca ggttgaggac
    33481 tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg caagtttgcc
    33541 caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac ccggctgcac
    33601 gggcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt tgttgatacc
    33661 tggcaggcag atgcagtacg cgatacccgt accctttccg gcggcgaaag tttcctcgtt
    33721 agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg tattgactcg
    33781 ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac cgcccttgat
    33841 gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca cgttgaagcg
    33901 atgaaagagc gtattcctgt gcagatcaaa gtgaagaaga tcaacggcct gggctacagc
    33961 aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa tacagagggg
    34021 cgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta ttggctacgg
    34081 tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt ttatctttgg
    34141 ctctgggcac gtttggtttg gggatggccg aatttggcat tatgggcgtg ctcacggagc
    34201 tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg tattatgcac
    34261 tgggggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac tcactcaaac
    34321 atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc acgctctctt
    34381 cgtcttacct gatgcttgcc attggtcgcc tggtttccgg ctttccgcat ggcgcatttt
    34441 ttggcgtcgg cgcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc accgccgccg
    34501 tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg ctgggaacgt
    34561 atttaagtca ggaatttagc tggcgttaca cctttttatt gatcgctgtt tttaatattg
    34621 cggtgatggc atcggtctat ttttgggtgc cagatattcg cgacgaggcg aaaggaaatc
    34681 tgcgcgaaca atttcacttt ttgcgcagcc cggccccgtg gttaattttc gccgccacga
    34741 tgtttggcaa cgcaggtgtc tttgcctggt tcagctacgt aaagccatac atgatgttta
    34801 tttccgtttt ttcggaaacg gcgatgacct ttattatgat gttagttggg ctagggatgg
    34861 tgctgggaaa tatgctaagt ggcaggattt caggacgtta ttcaccactg cgcattgcag
    34921 cagtgactga ctttatcatt gtactggcac tgctgatgct ctttttctgc ggcggcatga
    34981 aaacaacgtc gcttattttt gcttttattt gttgcgcggg attatttgcc ctttcagcac
    35041 ctctgcaaat attgttactg caaaacgcca aaggcggaga gttattaggt gccgcaggtg
    35101 ggcaaatagc gtttaacctc ggtagcgccg tcggcgcata ttgcggaggt atgatgctga
    35161 cgctggggct ggcatataat tacgtggcgc tgcctgccgc cctgctttcg tttgctgcga
    35221 tgtcatcgtt gctgctgtat ggtcgctata agcgccagca agcggcggat agtccggtgc
    35281 tggcgaaacc actggggtag attactgtca cggtgcgcgt tgcttattgc cggatgcggc
    35341 gtgaacgcct tatccgccct acgcggttct ggcacatttt gtaggcctga taagacgcgg
    35401 caagcgtcgc atcaggcatg atgcgccaat tgcctacgtt ttttactctt gtggccataa
    35461 ccacgcagcg ccgcgtacgc cgctggaatc accgtgcttc gccttacgca ccggcgtttc
    35521 acattcgccg ccgaagacaa attgtttaat caactgccca accgtttgat ataaacggtc
    35581 tacattgctc atcccgcccc ccaggacaat cacatccgga tcgagaatat tcacgacatg
    35641 tgccagcgat tttgccagcc gcagctcgta gcgacgcaat gccagttccg ctaccggatc
    35701 gctttcttca accaggcgga taatttcact gcctttcagc gcatgtccgc tcaaacgacg
    35761 ataatccgtc gcgaatcccg tgcccgaaat aaaggtttca atacaacctt gtttaccgca
    35821 ataacaaggg acttcctcgc gataacgcag ctcgtcttca tccatccacg gtagcggatt
    35881 gtgtccccac tcgcctgccg tgccattgcc gccgatatgc gcccgcccat ttaatgccac
    35941 gcccgcgccg catcccgtgc cgataatcac ggcaaatacc gtctgcgctc ccgctgccgc
    36001 gccatctact gcttctgaaa ccgccagaca gttggcatca tttgccagcc gcacttcccg
    36061 ctgcaacctc gcgcttaagt ctttatcgaa tggctgaccg ttgagccagg ttgaattggc
    36121 attcttcacc acaccggtgt agggcgaaat cgaacctgga atgcccatac ctaccgttcc
    36181 gcgctgccct gtcgcctgct ccgccatatc aaccaacgtg gcgatcgttt caatagtctg
    36241 ccggtaatca tcacgcggcg tgggcagacg atggcggtac aactgctccc ctgcatcgcc
    36301 cagtgcaatc acttcagttt tggtgccgcc taaatcgata cctatacgca ctgtactctc
    36361 cttatttttt tcaatatcaa tagcgtagag acggacaacc ggattggcaa tgcaaggccg
    36421 ccgacaattc gttatcatgc ccgctaaatt taacgacaag gccgtggaaa ttatcatgct
    36481 gtggttcaaa aatttaatgg tttaccgtct tagccgcgag atttcgctgc gtgcagaaga
    36541 gatggaaaaa cagctagcct cgatggcatt taccccatgc ggcagccagg acatggcgaa
    36601 gatgggctgg gttcctccga tgggatcgca cagcgatgcg ttaacgcacg ttgccaatgg
    36661 tcaaattgtt atctgcgcgc gcaaagaaga aaaaatcctc ccgtctccgg tgattaaaca
    36721 ggcgctggaa gcgaaaatcg ccaaactgga agcggaacag gcgcgtaagc tgaagaaaac
    36781 cgaaaaagat tcgctgaaag acgaagtgct gcactctctg ctgccgcgtg ctttcagccg
    36841 ttttagccag acaatgatgt ggatcgacac ggttaacggt ttgattatgg tggactgcgc
    36901 cagtgccaaa aaagcggaag atacgctggc attactgcgt aaaagcctgg ggtcgttacc
    36961 ggttgtacca ttgagcatgg aaaacccgat tgaactgacg ctgaccgaat gggttcgctc
    37021 cggtagtgcg gcacagggct tccagctgct tgatgaagcc gagctgaaat cgttgctgga
    37081 agatggcggc gtgatccgcg cgaagaaaca agatctgacc agcgaagaga tcaccaatca
    37141 cattgaagcc ggaaaagtgg tgactaaact ggcgctcgac tggcagcagc gcattcagtt
    37201 tgtgatgtgc gacgatggtt cgctcaagcg tcttaagttc tgcgacgagc tgcgcgatca
    37261 aaacgaagat atcgaccgtg aagatttcgc cctgcgtttt gatgccgatt tcatcctgat
    37321 gactggtgaa ctggcagcgt taattcaaaa cctgattgaa ggattaggtg gcgaagcaca
    37381 acgttaattg ctgattttcc tttaatgccg gatgcgacgc ctgccgcgtc ttatccggcg
    37441 tacgaagcca caccaggcat ataattattc gctacggcga gcaataattt ttagcgcagc
    37501 aatattatgc gttttacgcc gtaactgatc taatcgcctc atccctattc gcctgtcagc
    37561 agcacgcaga atattcacta acccccattt atcgctggta aattgttttg ttaacagctc
    37621 cgcttttggg gtatcaatat attcacgcaa taaatcagaa attgaacaaa tatccgtcag
    37681 gtaaggatac cagcaagttt caccgtgaaa attacgcact ccagcgttac ctgcaataaa
    37741 gtctttcggg taatcccaga tcacgttttt atccaacgtg atccaataac gcggtaaatc
    37801 ggtacttcca ccattttggc tgcgcatgag ataacgggta cagtgaattt gcaggttgat
    37861 cgttggcgtc agtaagtcat agatctcccg ctgtaacttg ctccatggac gttgtgtcat
    37921 tgtttttcct caagccggtg acgcgcttca ctgcgaattt tccgcgttgc cccggttttc
    37981 actaaatcac gtaactgcga aagatcgggt tgttcgacaa ataagagtaa tgatagatcc
    38041 tcttttaacc acgccgtttt cacatcggca gccagctgtg gattattaat agccagcgac
    38101 cgatcctgag actcagtcaa tgtcgttaac aacgacgccg gggtatgtgc gttgcgtaat
    38161 gccgtttggc gcaaatgatc attgcgactt tgcagcaatg cacgccacca ttcatcatct
    38221 gatttatctt ttatcgtagc cagttccgca aaaaatacct ggagatcttc actgatattt
    38281 atatcaacct tgatgaatta tgaatatatc ggaaattgaa aaaatcgcag caaagga
//
LOCUS       DAESHI010000033        37258 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.033, whole genome shotgun sequence.
ACCESSION   DAESHI010000033 DAESHI010000000
VERSION     DAESHI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37258
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.033"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..643)
                     /locus_tag="KW011_001694"
     CDS             complement(<1..643)
                     /locus_tag="KW011_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HBH7660049.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIKIRLIILFLL
                     AALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLK
                     NTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDES
                     GRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDL"
     gene            complement(743..1654)
                     /gene="gsiD"
                     /locus_tag="KW011_001695"
     CDS             complement(743..1654)
                     /gene="gsiD"
                     /locus_tag="KW011_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HBH7660050.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1657..2577)
                     /gene="gsiC"
                     /locus_tag="KW011_001696"
     CDS             complement(1657..2577)
                     /gene="gsiC"
                     /locus_tag="KW011_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HBH7660051.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2595..4133)
                     /gene="gsiB"
                     /locus_tag="KW011_001697"
     CDS             complement(2595..4133)
                     /gene="gsiB"
                     /locus_tag="KW011_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HBH7660052.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4153..6024)
                     /gene="gsiA"
                     /locus_tag="KW011_001698"
     CDS             complement(4153..6024)
                     /gene="gsiA"
                     /locus_tag="KW011_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HBH7660053.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6011..6976)
                     /gene="iaaA"
                     /locus_tag="KW011_001699"
     CDS             complement(6011..6976)
                     /gene="iaaA"
                     /locus_tag="KW011_001699"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBH7660054.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7180..8415
                     /gene="moeA"
                     /locus_tag="KW011_001700"
     CDS             7180..8415
                     /gene="moeA"
                     /locus_tag="KW011_001700"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBH7660055.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8415..9164
                     /gene="moeB"
                     /locus_tag="KW011_001701"
     CDS             8415..9164
                     /gene="moeB"
                     /locus_tag="KW011_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HBH7660056.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9343..10005)
                     /gene="fsa"
                     /locus_tag="KW011_001702"
     CDS             complement(9343..10005)
                     /gene="fsa"
                     /locus_tag="KW011_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBH7660057.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            10136..11035
                     /locus_tag="KW011_001703"
     CDS             10136..11035
                     /locus_tag="KW011_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBH7660058.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11041..13473
                     /locus_tag="KW011_001704"
     CDS             11041..13473
                     /locus_tag="KW011_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBH7660059.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13619..14434
                     /gene="ybiV"
                     /locus_tag="KW011_001705"
     CDS             13619..14434
                     /gene="ybiV"
                     /locus_tag="KW011_001705"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HBH7660060.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALNGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14586..15851
                     /locus_tag="KW011_001706"
     CDS             14586..15851
                     /locus_tag="KW011_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HBH7660061.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15818..16978)
                     /locus_tag="KW011_001707"
     CDS             complement(15818..16978)
                     /locus_tag="KW011_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBH7660062.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            17216..18517
                     /locus_tag="KW011_001708"
     CDS             17216..18517
                     /locus_tag="KW011_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH7660063.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWVHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            18532..20895
                     /locus_tag="KW011_001709"
     CDS             18532..20895
                     /locus_tag="KW011_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HBH7660064.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LGLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLDFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(21075..22670)
                     /locus_tag="KW011_001710"
     CDS             complement(21075..22670)
                     /locus_tag="KW011_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HBH7660065.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGVDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22889..23809
                     /gene="ldtB"
                     /locus_tag="KW011_001711"
     CDS             22889..23809
                     /gene="ldtB"
                     /locus_tag="KW011_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBH7660066.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23868..24986)
                     /locus_tag="KW011_001712"
     CDS             complement(23868..24986)
                     /locus_tag="KW011_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HBH7660067.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24983..25450)
                     /gene="mntR"
                     /locus_tag="KW011_001713"
     CDS             complement(24983..25450)
                     /gene="mntR"
                     /locus_tag="KW011_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HBH7660068.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25636..25764
                     /gene="mntS"
                     /locus_tag="KW011_001714"
     CDS             25636..25764
                     /gene="mntS"
                     /locus_tag="KW011_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HBH7660069.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            26036..27619
                     /gene="opgE"
                     /locus_tag="KW011_001715"
     CDS             26036..27619
                     /gene="opgE"
                     /locus_tag="KW011_001715"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBH7660070.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27668..28183)
                     /gene="ompX"
                     /locus_tag="KW011_001716"
     CDS             complement(27668..28183)
                     /gene="ompX"
                     /locus_tag="KW011_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HBH7660071.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(28236..28301)
                     /locus_tag="KW011_001717"
     CDS             complement(28236..28301)
                     /locus_tag="KW011_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660072.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            28536..29423
                     /gene="rhtA"
                     /locus_tag="KW011_001718"
     CDS             28536..29423
                     /gene="rhtA"
                     /locus_tag="KW011_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HBH7660073.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            29722..30225
                     /gene="dps"
                     /locus_tag="KW011_001719"
                     /gene_synonym="pexB"
     CDS             29722..30225
                     /gene="dps"
                     /locus_tag="KW011_001719"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HBH7660074.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            30629..31375
                     /gene="glnH"
                     /locus_tag="KW011_001720"
     CDS             30629..31375
                     /gene="glnH"
                     /locus_tag="KW011_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HBH7660075.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            31514..32173
                     /gene="glnP"
                     /locus_tag="KW011_001721"
     CDS             31514..32173
                     /gene="glnP"
                     /locus_tag="KW011_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HBH7660076.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            32170..32892
                     /gene="glnQ"
                     /locus_tag="KW011_001722"
     CDS             32170..32892
                     /gene="glnQ"
                     /locus_tag="KW011_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HBH7660077.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            33009..35234
                     /gene="ybiO"
                     /locus_tag="KW011_001723"
     CDS             33009..35234
                     /gene="ybiO"
                     /locus_tag="KW011_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HBH7660078.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(35231..36157)
                     /gene="rlmF"
                     /locus_tag="KW011_001724"
     CDS             complement(35231..36157)
                     /gene="rlmF"
                     /locus_tag="KW011_001724"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HBH7660079.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            36433..36693
                     /locus_tag="KW011_001725"
     CDS             36433..36693
                     /locus_tag="KW011_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBH7660080.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            36958..>37258
                     /locus_tag="KW011_001726"
     CDS             36958..>37258
                     /locus_tag="KW011_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBH7660081.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPG"
BASE COUNT         9033 a        10060 c         9338 g         8827 t
ORIGIN      
        1 acaaatcgtg atctttgccg ttgatacgta gcgtaaaaga gatacatttt ttacccggac
       61 gatcaaagca attgtatgcc ggattccagc tgttgttaga gtcatctgaa gccaccgacc
      121 acggtcgttt cgatgggtta aaatcggcaa aaataacccg ccctgactcg tcgacaagcg
      181 gtccatttac ttgttcctga tgataaacaa cgattttatc attcgccctc cgcgaaaaga
      241 gataatattt attatcatca agtaccagtc ccaccgagtc taaaaatggc atcatttcca
      301 ttttcagaat cacggcgcgt tgaatctcct ctgtattttt taataaatac agtgagtcct
      361 ttaatgtcag ggccgaacgt tcaatatctt taaagaaata gccaacgttt ttctgaataa
      421 gttcaaagcg gctatcggca cgttttaccg cgcgttccga taccagttga tagttaatga
      481 tgaaaaaaag accaataaat gacagtgctg ccaataaaaa taaaattatc agccttatct
      541 ttatcttttc gtataaactc agcatgttaa tacatttaac aaaataacta tctgaacgct
      601 gtgccgctgc aaaaatagtc ccttgctgcg gctttttaac caaattaccc gattctacaa
      661 aatcagcaga aagttacaac acttgcttag ggcaataatt tcctgtgagg tgattaccct
      721 ttcaagcaat attcaaacgt aactatcctt taattttcgg atccagcgca tcgcgtaaac
      781 catcgcccaa caaattgaac gccagtacgg tcagaaaaat agccagggcc ggaaaaacag
      841 cgacatgcgg cgcgataacc atatccgctc gtgcctcatt gagcattgct ccccactctg
      901 gtgtcggcgg ctgcgcaccg aggccgagaa atgacaggct ggcggcagag ataatcgagg
      961 tgccaatgcg catggtgaaa aacaccacga tagacgagac ggtcccaggc aagatatgac
     1021 gcaataaaat ggtcatatcg ctggcaccaa tactgcgtgc tgactcaata aaggtttgct
     1081 gtttcaacac cagcgtgttg ccgcgcacca gtcgggcaaa cgcggggatg gaaaaaatgg
     1141 cgactgcgat aatcacgtta gcaatgccgc ttcccaacac cgcaacaaca gcgatcgcca
     1201 gtaaaatacc cgggaaggca aacagcacat cgcaaatgcg catgatcagc cgatcccacc
     1261 agccttcata atatccagcc aacaagccca gcaacgtccc gatcgccgca ccgataaaca
     1321 cggcaaacac gcccgccgcc agcgagattt gcgcaccaac caggacacgg ctgaaaatgt
     1381 cgcgccccag tgaatcgacg ccaaaccagt gctgcaaaga aggtccgtta ttcagattgt
     1441 cataatcaaa ataattttcg gcgtcatagg gagcgatcca gcgtgcaaaa atggccacca
     1501 caatcaataa aataacgaat aatgcggcgg tcatcgccat atgctgacgg cgaaatcgtc
     1561 gccagaattc atgccacggt gtacgcacct ggtcaggttt gaccagtggc atggcgttta
     1621 acaccgcctg acgtcgccag ttaaatagtc gcatccttac ttgtacctga tagccgggtt
     1681 aatggcggcg taaagcacat ccaccactaa gttgataaga ataaattcca gcgagaaaag
     1741 cagaatttcc gcctgaatca ccgggtaatc gcgcatttct acggagtcaa cgagtaagcg
     1801 tccaagtccg ggccagttga aaactttctc caccacgata gaaccaccga gcaaaaagcc
     1861 aaactgtaag cccatcatgg tcactaccgg gatcatcgcg ttacgtagcc cgtgtttgag
     1921 gacaacccag gtttcgctca cccctttcgc cctcgcggta cgcatataat cttcgcttaa
     1981 aacatcgaca aacgacgcgc gggtaaagcg cgccatcacg gctgccactg ccgcgccgag
     2041 cgtcagggag ggtaaaatgt agtgctgcca actgtctgct cccacggtag gcagccagcc
     2101 cagttcaacg gagaatacct gaattaaaag catccccagc gcaaatgccg gaaacgagat
     2161 ccccgacacc gcaatggtca tactcaatcg atccggccaa cgattacgcc agacggcggc
     2221 gataattccc gccgccatac caaatatcac cgcccagacc atactggtta tggtcagcca
     2281 cagcgttggc ataaagcggc tggcaatctc atcggcaacc ggacgacgcg acaccatcga
     2341 caggccaaaa tccccctgca cagcattgct gatatagtgc cagaactggt gatacagcgg
     2401 ctgatccaac cccagctgct gacgcaccag ttctataacc tgcgcatcag cttcgagccc
     2461 ggcaatcaat cgcgccggat cgccgggcag catatgcaca aataaaaaca ccagcaccga
     2521 gacgataaac agcgtcggaa tcaaccccag taagcgtttg ataacgtaat taagcattcc
     2581 actccctgcg ttgcttattg caaatccgcg tcttcaaagc tgaagccggt gtctggcatg
     2641 atccaaaaac cggtcaggtt tttactgtgt gccgacacca gtttttctac caccagcggg
     2701 atccacggcg actcttgcca gatgatatcc tgcgccgcct tatacaagcg ggtcttttcc
     2761 gccggatcat tagttttcag tgcctgagcc aggaagtcgt ccacctgttt attgctgtaa
     2821 aatgcggtat taaacagcgt cggtggccag ttttgcgagg cgaacagtgg cgatagcgcc
     2881 cagtccgctt cgccggttga agccgaccag ccagtgtaga acatccgcac gccgctctct
     2941 ttttgccctt taccttcaac ttctgccgcc cgctgtccgg catccatcgc ggtcacctgg
     3001 gctttaatcc cgacctgcgc taactgctgc tgggtaaatt gcagcacttt ctgcgcggtg
     3061 ctgtggttat gtgacgacca cagcgtggta ctgaaaccgt tgggatatcc cgcctctttc
     3121 agtaattcgc gcgctttcac tggatcgtaa ggccacggtt tataactttg cgcgtaggcg
     3181 atacttggcg gtaccacacc agtagctggc gttgcatagc ccgcaaaggc aactttcacc
     3241 agcgccggac ggttaatggc gtaattcagc gcctcacgga ccttcgggtt atcgaacggc
     3301 ttttgcgtca cgttcatact gatataacgc tgcataattg acggactggc catcaactcg
     3361 atatttttgt ttttctccag cagtgtggcc tgctcgtagg gaatggggaa agcaaactgc
     3421 gcttcaccgg tttgcagcat tgccgcgcgg gtgttgttat ccgccaccgg acgccaggtt
     3481 atgctgtcca gtttgggcaa tcctggctgc cagtaacccg cgaatttttt caccttcaca
     3541 aaatcggtct gattccaggt atccagttca tacggtccgg ttcccaccgg atgaaaacca
     3601 atctccttgc catatttttc cagcgctgcc ggtgaaatca tcgcggtcgc cggatgggca
     3661 agaatattaa taaacgctga gaacggctgt ttgagggtaa tctttaccgt tgtcggatcg
     3721 atcgcctccg ttttggcaat attcttatac aggttatagc gtttaagatg attcgccgga
     3781 tcgctggccc ggtccagatt cgcttttacc gccactgcgt tgaaatcggt gccatcctgg
     3841 aatttaatgc cttcccgcaa tttcacggtg taagtaatgc cgtcatcgga aacggtataa
     3901 ctctccgcca gcacgttttt cagtttcatc tctttatcca gaccgaacag cccctggtaa
     3961 aacgatttcg ctacggcctg agataacgtg tcattcgcgt catacggatc gagcgtggtg
     4021 aaattcgatc ctaccgccac caccacatct ttggcagcga atgcacaaga tgccatcaac
     4081 gctgtcgcaa tgcccagcgc cactaaccca ctacggtgta cagctcttgc cattttattc
     4141 tccgcctgaa tgttatctac gcatgaatgc gtattctgat tgtggttgtg cgacgtaatg
     4201 ccccggcccg acgcattgca acgagacggc tgccacctct tcgccacgca gatgaatatt
     4261 gctgggaaga tcgtccgaca gcagtacacg ctgcggtcgt tgtcgggacg gttcagcgac
     4321 cggaactgcc gccagtaatt tacgcgtata aggatgctgc gggttttcga agaccgcgcg
     4381 ccgtggacca atttcaacaa tttgcccgag atacatcacc gccacacgat gactaatccg
     4441 ctctaccacg gccatatcgt gggagataaa cagatacgca atgccgaaat cacgctggag
     4501 atcgagcaac aagttgataa tctgcccgcg aatagaaaca tccagcgccg aaacggcttc
     4561 gtcggcaatg atcacttttg gattcaatgc caacgcgcga gcaatgcaga tgcgctggcg
     4621 ctgaccgccg gaaaactcat gcgggtaacg ccaggcatgt tcaggtaaca ggcccacgcg
     4681 ctccagcaac cacgcaacgc gtgcagccgc gtctttacct ggcaataaac cgtgtacacg
     4741 cagcggttcg ataatcgaat caccgatggt ctgacgtggg tccagcgaag cgtaagggtc
     4801 ctgaaaaata aactgaatat cccggcgcaa tgcctgaagt ttgccgggtg acaaggtatc
     4861 gattctctga ccgttaaaga taatttcgcc gccctgcgat tcgaccaggc gcagcaacgc
     4921 ccgcccggta gtggatttac cgctgccaga ctcgcccacc agcgatagcg tttcgccagg
     4981 ccagagatca aaactgactt tctcaacggc atgcacttcc cgcgttacgc gattcaacaa
     5041 accgctgcgc aaagggaaac gggtgacaag attacgcact cgtaaaacag gttcgccatc
     5101 caccaccgtt ttctgctcga tggggggggc ctgtttcgct ggatgttcaa gcgatatcaa
     5161 cgggaaacgt cggggataat ccaacccttt catcgcacca agttgcggaa cagcagctaa
     5221 cagcgcacgg gtgtaaggat gttgcggtgc atgaaaaatc tgttcgacgg tacccgtttc
     5281 caccgcctcg ccctgataca tcaccagtac ccgatcggca atctctgcca ccacgcccat
     5341 atcgtgagtg ataaagataa cgcccatcga catctctttt tgcaatactt tgattaattg
     5401 caggatctgc gcctgaatag tgacatccag cgcggtggtt ggctcatcgg caatcagcac
     5461 cgccgggcgg catgacagcg ccatcgcaat catcactcgc tggcgcatcc cgccagagag
     5521 ttgatgcgga taacgtgaaa gaatggtttg tgcctcagga atgcgtacct gatccagcat
     5581 ccgcttcgcc tcgaccatcg cttcttcacg actggcgttc tgatgcagac gaattgattc
     5641 ggcaatctgt tcacccacag taaataccgg gttcagcgat gtcatcggct cctgaaatat
     5701 catcgccata tccgcaccgc gaacatggcg catttgtgca gcgctctgct cgctaagttc
     5761 aatcacttcg cgactgcgcc gctgcaacag cattttatcg cactgtacta aaccgcccgc
     5821 ctgttccaac aggcgcatca atgccaacgc agtcactgac ttaccggagc cggattcgcc
     5881 aacaattgcc agcgtctcac cgcgttgcag actaaaagag agattgcgga ccgcagctat
     5941 tttctgctgg tcctgcataa aggcaatatt cagattttca accgccagca cattaccggc
     6001 atcaagttca tcactgtgtg gcaacggtgt cccctttttc acggtagata ccggtggttg
     6061 gcgtatcgcc tgcgtagccc caggcgcgat acattccttc ggtgttaaac ggtagcgcga
     6121 cattcccttc atggtcgata gcgattaagc caccgctacc gccaagcgca gggagttttt
     6181 ccattactac ccgctcgcag gcttccgcga gacttaatcc gccgtaatcc attaacgcgg
     6241 cgatgtcata tgccgccagc gcgcggatga agacttcgcc cgtgccgata caagaaaccg
     6301 ccacactggc gttattggcg tagcatccgg cacccactaa gggactatcg ccaactcgtc
     6361 cgggtaattt attggtcatt ccgcctgtgg acgtggctgc cgccaaattg ccgtctaaat
     6421 ccaacgccac ggcccccacg gtgcccattt tctgtttttc atccagtggc gcaccgctat
     6481 ggtcgaggac ggttgcccct tccttgcgcg ctgccagtag ttgttcataa cgcaaaggcg
     6541 tggagaaaat ctccggcgag acgcgctcca tgccacgagc aaacgcaaaa ttttctgccc
     6601 cttcgccaat catcatcaca tgcgggcttt gctccatcac cagccgggcg gcaagaaccg
     6661 gattacgcag atgactaacg cccgccaccg caccggcttt cagggtgtta ccatccatca
     6721 cacaggcgtc cagttcatgg gtttcatcac gcgtaaagac agcgccaatt ccggcgttaa
     6781 acagtggaca ctcttccagc agacgcaccg cttccgtcac cacatccagc gcactttcgc
     6841 ccgcttccag cattttctgc ccggtttcaa caatggcaga caacgcctca atgtagcgta
     6901 attcctgttg cagactcatc tgcgcgcggc taattgcacc tgcgccacca tgaattgcaa
     6961 tgactgcttt gcccatagta ttcgtccatt aagggtggct cgccactttt gctataaata
     7021 tcagaatttt tatgaattac tcattcaatt tttgaatata gaaagatgga tatggcatgt
     7081 aaaggcagga gccaacgcta cgcccgaggt ggcatacagt ctgtggcgtt tttctgacat
     7141 aataggcaaa ttcgattttg cctccgcagg agtgttttca tggaatttac caccggattg
     7201 atgtcgctcg acaccgcgct taatgagatg ctttctcgcg tcaccccact gaccgcccag
     7261 gaaacgctgc cactggtaca gtgttttggt cgtattctgg cgagcgatgt cgtttcgccg
     7321 cttgatgttc cggggtttga taactccgca atggacggct acgcggtgcg tttagccgat
     7381 attgcctccg ggcaaccgct gcccgttgcc ggtaaatcct ttgccggtca gccgtatcat
     7441 ggtgaatggc ctgcgggcac ctgcattcgt attatgaccg gtgcgccggt gccggaaggc
     7501 tgcgaagcgg tggtgatgca ggagcagact gaacaaacgg acaatggcgt gcgttttact
     7561 gctgaagtgc gtagcgggca aaatattcgc cgtcgcggtg aagatatctc tgcaggtgcg
     7621 gttgttttcc cggcgggaac tcgcctgact accgcagagc tgccagtaat tgcttcactg
     7681 gggattgccg aagttccggt gattcgtaaa gtgcgtgtag cgcttttttc taccggtgat
     7741 gaactccagt tgcccggtca gccgctgggc gacggccaaa tctacgacac caaccgtctc
     7801 gccgtacacc tgatgttaga acagctgggt tgcgaggtaa tcaacttagg gattatccgc
     7861 gacgatcccc atgccctgcg cgccgcattt attgaagccg acagccaggc ggatgtagtg
     7921 atcagttccg gcggtgtttc agtgggtgaa gcggattaca ccaaaacgat tcttgaagag
     7981 ctgggggaga tcgccttctg gaagctggcg attaaaccag gtaaaccgtt cgcgttcggt
     8041 aaactcagca atagctggtt ctgcggcctg ccgggcaacc cggtttcagc gacgctgacc
     8101 ttctatcaac tggtacagcc tttgctggca aaactaagcg gcaacaccgc cagcggcctg
     8161 cccgcgcgcc agcgcgtacg cacagcgtcc cgcctgaaaa aaacgccagg acgtcttgat
     8221 ttccagcgcg gcgtgctgca acgcaacgcc gacggtgaac ttgaagtgac gaccaccgga
     8281 catcagggtt cacatatatt tagctccttt agtctcggca actgctttat cgtgctggaa
     8341 cgcgatcgcg gcaatgtgga cgtgggcgaa tgggtggaag tagaaccgtt taacgcgttg
     8401 ttcggaggcc tgtaatggcg gaactcagcg atcaggagat gctgcgctac aaccggcaaa
     8461 tcattctgcg cggttttgat tttgacggtc aggaggcgct aaaagattct cgtgtgctga
     8521 tcgtcggcct cggtggactc ggctgcgcag cttcgctgta tctggcaagc gccggtgtcg
     8581 gcaacctgac gctgctcgac ttcgacacgg tttcgctctc gaatctgcaa cgccagacac
     8641 tgcacagtga tgccacggtc gggcaaccga aggtggaatc cgcccgtgac gccctgacgc
     8701 ggatcaaccc acatatcgcg attacgccag tcaatgcact gctagatgac gcagaacttg
     8761 cagcattgat tgctgaacac gatctggtgc tcgactgtac ggataacgtt gcggtacgta
     8821 atcaactgaa cgcaggctgt tttgccgcga aggtgccgct ggtttccggc gcggcaattc
     8881 gtatggaagg gcaaatcacg gtcttcactt atcaggacgg cgaaccgtgc tatcgctgcc
     8941 ttagccgttt gtttggcgag aacgcattaa cctgcgtaga agcaggcgtg atggcaccgt
     9001 tgatcggcgt aattggttcg ttgcaggcaa tggaagcgat caaactgctg gcaggttatg
     9061 gcaaaccgac cagcgggaaa atcgtcatgt acgatgcgat gacctgtcag tttcgcgaaa
     9121 tgaaactgat gcgcaatccg gggtgtgagg tgtgcgggca gtaattgcca cctccaggcc
     9181 tgataagacg cgacaagcgt cgcatcaggc atttgctcca gacgccagat gcggcgtgaa
     9241 cgccttatct ggcctacggt tcgggcacag atttccaagc gcggcaggca tcggtgcact
     9301 ccgccacgtc atcagcaatt tatgaggatg cagaacgtcc ggttaaatcg acgttctgcc
     9361 aaacgctccc tgccagtcct gctcaaactt cgccacagcg gcttccaccg ccggatagct
     9421 aatcatctgt tgtgccacat ccagtggcag agtaattgat tcacatcctg ccagtaagca
     9481 gtccagcgcc tgacgcgggg ttttgaaact cgctgccagc actttcgcct gcggcgcatg
     9541 cattttcaat aactggtgta agtcggtcac agtctgaatg ccgctaccgc cctgagcatc
     9601 aatacgatta acgtaaggcg caacatattc cgcacctgcc agcgccgaca gcaacccttg
     9661 tgctgcgcca tataccgcgg ttcccagcgt tggaatccct tccgctttta acatcttaat
     9721 agctgccagc ccctcggcgg tcaccggaac tttcaccacg atatccgcaa taatagaacg
     9781 caatttacgt gcgtcattca ccatcccttc agcagtggta gccattacct gggcaaacag
     9841 acgcccctga ccgcccatcg cttcatgaag ttgcggaagc acaacatcca gcggtttttt
     9901 acccgcggcg ataatgcttg ggttagtggt cacacccgcc agcggaaaaa tacgtgacag
     9961 cgccttcacc gcaacaacgt ctgaagtatc cagatacagt tccataacca tcctcaaaat
    10021 gtactttttg tctgaatgta gactatcacg ggaaaactct ctgaacagtt gacgcacatc
    10081 aatctaactt tcattcgaaa gtattttaat gtttgaacga aacagagagg caactatgat
    10141 tttcaatatt cagcgctact cgacccatga tggccccggt atccgcacgg tcgtatttct
    10201 taaaggctgt tcgctgggct gccgctggtg tcagaacccg gaaagccgcg cccgcacgca
    10261 ggatctgctg tatgacgcac gactgtgtct ggaaggctgc gagctgtgcg ctaaggccgc
    10321 gccggaagtg attgagcgcg cgctgaatgg tttgcttatt catcgggaaa agttaacccc
    10381 ggagcatctg acggcgttaa ccgactgctg tccgacacag gcattaaccg tgtgtggtga
    10441 agtgaaaagc gttgaggaga tcatgacgac cgttctgcgc gataaaccgt tttacgatcg
    10501 cagcggcggc ggtttaacgc tttcgggtgg tgagcccttt atgcagccgg aaatggcgat
    10561 ggcgctactg caagccagcc acgaggcagg cattcatact gcggtagaaa cctgtctgca
    10621 tgtgccgtgg aaatatatcg ccccttctct gccctatatc gatctgtttc ttgccgattt
    10681 aaaacacgtt gccgacgcgc cgtttaaaca gtggaccgac ggtaacgccg ccagagtgct
    10741 ggataacctg aaaaaactcg ccgcagcggg caaaaaaatc attatccgcg tgccgctgat
    10801 tcagggcttt aatgccgacg aaacctctgt aaaagctatt accgattttg ccgccgacga
    10861 gctgcacgtt ggcgaaattc atttcctgcc ctaccacacg ctaggcatca acaaatatca
    10921 cttacttaat ctgccctatg acgccccgga aaaaccgctt gatgcgccag aactgctcga
    10981 ctttgcccag cagtatgcct gccagaaagg gttaaccgcg accttacgag gataacaacc
    11041 atgaccacac tgaaactgga cacgctcagc gaccgcatta aagcgcacaa aaatgcgctg
    11101 gtgcatattg tgaaaccgcc agtctgtacc gagcgcgcgc agcactatac cgagatgtat
    11161 caacaacatc tcgataagcc gatcccggta cgtcgcgcgc tggcactggc gcatcacctg
    11221 gcgaatcgca ccatctggat caaacacgat gagttgatca ttggcaacca ggcaagcgaa
    11281 gttcgcgccg cgccgatctt cccggaatat actgtctcgt ggatcgaaaa agagattgat
    11341 gatctggcag atcgtcccgg tgctggcttt gcggtgagcg aagagaacaa acgcgttctg
    11401 catgaagtgt gcccgtggtg gcgcggtcag accgtacagg atcgctgcta cggcatgttt
    11461 accgatgagc aaaaaggtct gctggcgacc ggaatcatta aagcggaagg caatatgacc
    11521 tccggcgatg cgcacctggc ggtgaatttc ccgctgctgc tggaaaaagg tcttgatggt
    11581 ctgcgcgaga aagtagcgga acgtcgctcg cgcatcaatc tgacggtact ggaagatttg
    11641 cacggcgaac agttcctgaa agcgattgat atcgtgctgg tggcagtcag cgaacacatt
    11701 gaacgttttg cagccctggc gcgtgaaatg gccgcgaccg aaacccgcga aagccgtcgc
    11761 gatgaactgc tggcgatagc agaaaactgc gatcttatcg cccaccagcc gccgcagact
    11821 ttctggcagg cgctgcaact gtgttacttc atccaattga tattgcagat cgaatctaac
    11881 ggtcactcgg tatcgtttgg tcgtatggac cagtatctct acccgtacta tcgccgcgac
    11941 gttgaactca accagacact ggatcgcgaa cacgccatcg aattgctgca tagctgctgg
    12001 ctgaaactgc tggaagtgaa caagatccgc tccggctcac actcaaaagc ctctgcggga
    12061 agtcctctgt atcagaacgt caccattggc ggacaaactc tggttgatgg tcaaccaatg
    12121 gacgcggtga atccactctc ttacgctatc ctcgaatcct gcggtcgcct gcgttccact
    12181 cagcctaacc tcagcgtgcg ttaccatgca ggaatgagta atgatttcct cgacgcctgc
    12241 gtacaggtga tccgttgcgg cttcgggatg ccggcgttca acaacgacga aatcgtgatc
    12301 ccggaattta ttaaactcgg tattgaaccg caggacgctt acgactacgc agcgattggt
    12361 tgtatcgaaa ccgccgtcgg tggcaaatgg ggctatcgct gtaccggcat gagctttatc
    12421 aacttcgccc gcgtgatgct ggcggcgctg gaaggcgggc gtgatgccac cagcggcaaa
    12481 gtgttcctgc cacaagaaaa agcgttgtcg gcaggcaact ttaacaattt cgatgaagta
    12541 atggacgcgt gggatacgca aatccgttat tacacgcgta aatcaatcga aatcgaatat
    12601 gtcgtcgaca ccatgctgga agagaacgtg cacgatattc tctgctcggc gctggtggat
    12661 gactgtattg agcgagcgaa aagtatcaag caaggcggcg cgaaatatga ctgggtttct
    12721 ggcctgcagg ttggcattgc caacctcggc aacagcctgg cggcagtgaa gaaactggta
    12781 tttgaacagg gcgcgattgg tcagcaacag ctggctgccg cactggcgga tgacttcgat
    12841 ggcctgactc atgagcaact gcgtcagcgg ctgattaacg gtgcgccgaa gtacggcaac
    12901 gacgatgata ccgtcgatac gctgctggct cgcgcttatc agacctatat cgacgaactg
    12961 aaacagtacc ataacccgcg ctacggtcgt ggtccggttg gcggcaacta ttacgcgggt
    13021 acctcgtcta tctccgctaa cgtaccgttt ggcgcgcaaa ctatggcaac accggacggg
    13081 cgtaaagccc acaccccgct ggcagaaggc gcaagcccgg cctccggtac tgaccatctt
    13141 ggccctactg cggtcattgg ctcagtgggt aaactgccta cggcagcgat tctcggcggc
    13201 gtgttgctca accagaaact gaatccggca acgctggaga acgaatctga caagcagaaa
    13261 ctgatgatcc tgctgcgtac cttctttgaa gtgcataaag gctggcatat tcagtacaac
    13321 atcgtttccc gcgaaacgct gctggaggcg aaaaaacatc cggatcagta ccgcgatctg
    13381 gtagtgcgtg tcgcgggcta ttccgccttc ttcaccgcgc tctctccaga cgcacaggac
    13441 gatatcatcg cccgtactga acatatgctg taatctcctc ggcccggcgg cgtcgtcgcc
    13501 gggctaaatc attcacatca tcaatttcat ccttactttc attcgaaata taatttgtgc
    13561 tctgcgtcac attgttatta cactgttacg gtttcatttt cgagccagga gcacacctat
    13621 gagcgtaaaa gttatcgtca cagacatgga cggtactttt cttaacgacg ccaaaacgta
    13681 caaccaacca cgttttatgg cgcaatatca ggaactgaaa aagcgcggca ttaagttcgt
    13741 tgtcgccagc ggtaatcagt attaccagct tatttcgttc tttcctgagc taaaggatga
    13801 gatctctttt gtcgcggaaa acggcgcact ggtttacgaa catggcaagc aactgttcca
    13861 cggcgaactg acccgacatg aatcgcggat tgttattggc gagttgctaa aagataagca
    13921 actcaatttt gtcgcctgcg gtctgcaaag tgcatatgtc agcgaaaatg cccccgaagc
    13981 atttgtcgca ctgatggcaa aacactacca tcgcctgaaa gctgtaaaag attatcagga
    14041 gattgacgac gtactgttca agttttcgct caacctgcca gatgaacaaa tcccgttagt
    14101 gatcgacaaa ctgcacatag cgctcaatgg cattatgaaa cccgtcacca gtggttttgg
    14161 ctttatcgac ctgattattc ccggtctaca taaagcaaac ggtatttcgc ggttactgaa
    14221 acgctgggat ctgtcaccgc aaaatgtggt agcgattggc gacagcggta acgatgcgga
    14281 gatgctgaaa atggcgcgtt attcctttgc gatgggcaat gctgcggaaa acattaaaca
    14341 aatcgcccgt tacgctaccg atgataataa tcatgaaggc gcgctgaatg tgattcaggc
    14401 ggtgctggat aacacatccc cttttaacag ctgacctctc taccggagcc gtgcgctccg
    14461 gttttccctt ctttttacat cctgtacatc acatcaaatc attaaacttg cattaactta
    14521 tttataactt atattgtcgc atgcaaatca gaatggtttc gaatgaaagt taatgagagg
    14581 tggttatggc ttctactttt accagcgaca cattgcctgc cgatcacaaa gcagctatcc
    14641 gtcagatgaa gcacgcgctg cgggcgcagc ttggcgacgt ccagcagatc tttaatcagc
    14701 taagcgatga cattgccacg cgagtggctg aaatcaacgc actcaaagca cagggcgatg
    14761 ccgtctggcc ggtgctgtct tatgccgata tcaaagcagg tcatgttact gcagagcagc
    14821 gcgaacagat taaacgtcgc ggttgtgcgg tgataaaagg ccatttcccc cgcgaacaag
    14881 cgctaggctg ggatcagtcg atgctggact atctggaccg caaccgcttt gacgaggtct
    14941 acaaaggccc cggcgataat ttcttcggga cgctcagcgc ttcacgtccc gagatttacc
    15001 ccatctactg gtcgcaggcg caaatgcagg cccgccagag tgaagaaatg gcgaatgcgc
    15061 agtcgtttct caatcgtctg tggacatttg aaagtgatgg aaagcaatgg tttaacccgg
    15121 atgtgagcgt catctaccct gaccgtatcc gccgccgtcc gcccggaacg acctccaaag
    15181 gtcttggagc gcataccgac tccggggcgc tggaacgctg gctgcttcca gcgtatcagc
    15241 gcgttttcgc caacgtcttt aatggcaatc tggcgcaata tgatccctgg catgcggcac
    15301 atcgtacgga agttgaagag tacacggtgg acaacaccac caaatgttcc gtgtttcgga
    15361 cattccaagg ctggacagcg ctctctaata tgctgcctgg tcaggggctg ctgcacgttg
    15421 tgcccattcc tgaagccatg gcgtacgtac tgttacgtcc gctgcttgat gatgtgccgg
    15481 aggatgaact gtgcggcgta gcgcccggaa gagtgttgcc ggtatcagag caatggcatc
    15541 cactgttaat tgaggcgtta accagcattc caaaactcga ggccggagac tccgtctggt
    15601 ggcactgcga cgtcatccat tccgttgccc ccgttgaaaa tcaacagggt tggggcaacg
    15661 tgatgtacat tcctgcggca ccaatgtgcg agaaaaatct tgcctacgcg cacaaggtga
    15721 aggccgcact ggaaaaaggc gcatcgccgg gcgacttccc gcgcgaggac tatgaaacaa
    15781 actgggaagg acgctttacg cttgccgacc tcaacattca cggtaagcga gcgttgggaa
    15841 tcgacagctg agtgtcgtac agcccttccc gttccacggc ggtgttccct tttcctgggc
    15901 gaacccgccg cactgactcc cgcaccgcca atcgtccgtg cagcagcagc gagcccaccg
    15961 gagcatccgg gcgaatcagc cgctgttgca acatttgcac cgcttcattg cctagctcat
    16021 cgcgtggcac atgcacagcc gtcaggggaa catcctcgat tgccgcaagg ttaaagccgt
    16081 cgatactcat caccgagaca tcctgtggta cgcgcagacc acgacgatgt agcgcattta
    16141 ccgttcccgc cgccataaaa tccccgccga ccagaaacgc cgtcggcaac gtcttgccat
    16201 tggcggcatc cagccacgct ccgacctttt cttccgcttc tttagcgcta aaactgctga
    16261 tattcagcaa atgacggtca tggtgaaagg ccagattatg ctgacgccat gattctttaa
    16321 tccctgcaaa ccgctgctcc atagtgtagc ggcgcaggca catcacattc atcacctccc
    16381 ggtgacccat ttcaaacaga tagcgggcgg cgaacgcgcc aattaaccga tgatccggcg
    16441 caatcgccgg gaggcgcatt cgttcatcac ggcagttgat cagcacacaa ggcttagcga
    16501 gatcggcggc cagatcatgg atgtgcggat cgtcaatccc cagcaggatc gctgcctgcg
    16561 tatctgcttc attcatccgc gagagaaaga gcgcgggatc gctgtcaaac tcctccagcg
    16621 cgcagtaacg cagccgcact tcatgcggcg ataacgcttt gctgatgctc tggatcacac
    16681 ggtaataaaa gatatcagat cgttggtcaa aagcgcgttg cggcgcgaaa ataatcaggt
    16741 tgttcagcaa catacgacct gctgccatgc catccatcac gcccaactca cgcgcgcagg
    16801 ccagcacctg ttcgcgagcg cgtgcactgg tattcgcttt gccagccagc acccgcgaaa
    16861 ccgtactcgg cgatagcccg gtacgtgcgg cgatatcggc gattttcagc ttcttgtcca
    16921 ttttgtgatc tgcctctaat tcggaaatga cagaattttc atgagcaaaa aagcccattc
    16981 tgtctggcat taaatccatt tcagtaatgt atatcagtca ctcatggaaa aacgtgcaca
    17041 aaacccgctt tctttcgctt ttagggtctc ttactgtgaa ttggtagaac aacttccata
    17101 caaaaataat ggaaaaacga acaataaaga ctttatttat caaaaagtta aaagtggtgt
    17161 aacaactcca ttctgacgtt gccgcacaaa ccctatacca ttctgtggag tgcatatgag
    17221 tcagggtatc aataacgata tgactgccag taaaccgcgc catatcgtca aaaatctgcg
    17281 ttggtggata ctggtccttt ttttgctcgg tgttactgtc aactacatca cgcgtaactc
    17341 gctgggtatt ctcgcccccg aactgaaaag cagcctaggg attaccaccg aacaatattc
    17401 ctggattgta ggtgctttcc agctcgccta taccctattc caaccgttgt gcggatggct
    17461 gattgacgtc attggcctca aactgggctt tatgatctgc gcgggactgt gggcactggc
    17521 ctgtctgatg catgcggggg cgggaaactg ggtacatctg gcgctgttgc gcttctttat
    17581 gggcggtgcg gaagccgcgg caaccccagc aaacgccaaa accatcggtg aatggtttcc
    17641 aaaatcagaa cgcccgatcg ccgcaggctg ggcaggcgtc ggtttttcca tcggtgccat
    17701 gctggcccca ccgattatct attttgccca cgcttcattc ggctggcagg gtgcatttat
    17761 gttcactggc atcctggcga tgctgtgggt ggtgctgtgg tgggcgttct atcacagccc
    17821 ggataagcat cctaacctca gcaaagccga gctggacttt attcgccagg ataacgaagc
    17881 gcccccggtc aagttaccct ttctgaccgc actgaaaacc gtgggcaaaa ataaacgctt
    17941 ttacggtatc gctatcccgg cctttatggc agaaccggcg tgggcggtga tgagtttctg
    18001 ggtgcctcta tacctgtcca aaacctatgg catggacctg aagcagatcg cattattcgc
    18061 ctggttgccg ttcctcgccg ctgatcttgg cagtattgcc agcggctatc tgaccaaact
    18121 ttacacccgc ctgtttgggc tgagtcgtgt gaactccgtt gtcgcatcat cggtcagtgg
    18181 cgcattcttg atggtttcgc tggcggtgat ggcctttacc aataacccgt atatcgccat
    18241 cgtccttatc tccatcggcg gcttcggtca ccagatcatc tcctgcatgc tgagcgcact
    18301 ggtggtggaa tccttcgaca aaggtcagat ggccactgtt aacggcatgc gcggttctgc
    18361 cgcgtggatc gcaagtttcc tgttctccct gattatcggg gttaccgctg acaaaatcgg
    18421 ctttaacccc ctgtttatcg ccatgggctt ctttgacctg attggcgctt tcttcctggt
    18481 cacctttatt gctgaacgtc gcgcaaaacg cgcctgagag tggatgtaaa catgaaaacg
    18541 ctgaaaaact ggaccctgca caaacagtct gcccaccacg ttgaactgct ggtcgatggc
    18601 cagcatatcc tgtgcctgta cgtgctggaa gaaaatctgt tccgcgtgct gttaaaacgc
    18661 aaaggcgagc tggcgctaga tcgcacctgg agcattgctc cggcgcagga tgtgccatgg
    18721 gaagggcgca gccgcgaaga tctcagcggc tttagcctgc ccgcgtggac agtgatccag
    18781 cacgacgaca ccgtggttat cgccaccgag tcactgcgtg caaccgttca ccagccgctg
    18841 gggctggagt ggcactatcg taacaacgaa ggcgaatggc tgccgttggt gaatgaccgc
    18901 ccgaccagcg cttatctgct gaatgcccac ggcgatggcg tggcacacta tcagagccgt
    18961 cgtaaagatg aacgctttta tggcctcgga gataaagccg gcgatctgca acgcaacggt
    19021 aaacgttacg agatgcgtaa cctcgacgcg atgggctaca acgccgttag tactgacccg
    19081 ctgtacaaac acattccatt caccatcacc cgccgcgatg acattagctt cggcctgttt
    19141 tatgacaacc tcagcagctg ctggctggat ctgggcaacg agatcgacaa ctaccacacc
    19201 gcctatcgcc gctggcaggc ggaagcgggt gatatcgact actacctgtt taccggcgaa
    19261 cgcgtacttg atatcaccaa agccttcgta cgcctgaccg gaaaaaccct gttcggaccg
    19321 aaatggagcc tgggctacag cggttcaact atgcactaca ccgatgcgcc ggacgcacaa
    19381 aatcagttga tgaactttat ccgcctgtgt gaagaacatg caattccctg cgattcgttc
    19441 cagctctctt ccggttatac ctcaattaac ggcaaacgct atgtgttcaa ctggaactac
    19501 gacaaagtgc cgcagccgaa agtgatgagc caggcatttc acaatgctgg tttgcacctg
    19561 gccgccaata tcaagccgtg cctgttgcag gatcacccac gctataacga agtggcagaa
    19621 agaggactgt tcatccgtga ttcagaaaca gatgaaccag aacgttccag cttctgggat
    19681 gacgaaggtt ctaacctcga ctttactaat ccgcagacca tccagtggtg gcaggaaggc
    19741 gtgaccacac agcttctgga gatgggtatc gactccacct ggaacgacaa caacgaattc
    19801 gaagtatggg acggtgaagc acgctgtcac ggctttggtc agacgatcgc gatcaaacat
    19861 attcgcccag tgatgccact gctgatgatg cgagcctcga tggaagccca gcagcgtttc
    19921 gctccggaaa aacgtccgta cttgatctcc cgctccggct gcgcgggaat gcagcgctac
    19981 gtgcagacct ggagtggcga taaccgcact agctgggaca ctctgcgcta caacacccgc
    20041 atgggtctgg ggatgagcct ctccgggctg tataacctcg gtcacgacgt cggcggtttc
    20101 tccggtgata agccggaccc ggaactgttt gtacgctggg tacaaaacgg cgtgatgcat
    20161 ccgcgcttta cgattcactc atggaacgat gaccataccg taaacgaacc gtggatgtat
    20221 ccgggcgtca ctccggcgat ccgcagcgct attgagttgc gctatcgcct gctgccctac
    20281 ttttataccc tgatgtggca ggcatacgcc gatgatgaac caatgctacg tccgactttc
    20341 cttgaccacg agcacgacgt tcagactttt gaagagtgtg atgacttcct gcttggtcgc
    20401 gatattttgg ttgccagcgt cgtagagcaa ggcgagcgcc agcgccgtgt atggctgcca
    20461 gataacgaaa ctggctggta cgatttctac aacggcgaat ggttcagtgg cggtcagtgg
    20521 atcaccctcg acgcaccgct ggagaaactg ccgctgatgg ttcgtgccgg ggctggtcta
    20581 ccgctcagca aacgcatcac ctatgtgagc gcagaacaag acgatacccg cgaactgaaa
    20641 ctgttcccgc tgaaaggtgt gggcacaacg tccggcctgc tgtttgaaga cgatggcgaa
    20701 agctggggct accaaactgg taatgcgctt tgggtggaat gggaaatggt ttgcgatggg
    20761 gcgaccatca acctgaaagt caacgcgcgc ggggattatc gtcctgcatg gagtgcactg
    20821 aaagtgtcat taccattgga agaaaaacgc acgctgctgg tcaatggtgt tgaagggagt
    20881 gaatggatgc gttaaatcat taccggggcc gggaggcgac tgcaccttac ccggtccagc
    20941 catcgaatcc tttacctcag tatttcacgc tgcaggaagg cagcaaacct gtaaaccccg
    21001 ggagcttaaa taagtaagtg accggggtga acaggtgaag ccaatgcatc tgccgcatga
    21061 aagacggcgg ggaattaagc gtcgatccct ttactacgca ggtaatcttc gtaattaccg
    21121 ctaaagtcga tcgcgcgttc cggggtgatt tccagaatgc gggtcgccag ggagcttacg
    21181 aactcacggt cgtgtgaaac aaagatcagc gtgccctgat acagttccag cgccatgttc
    21241 agtgactcga tggattccat atccaggtgg ttggtcggtt cgtccatgat cagaatgttc
    21301 ggcttctgca tcattaactt accaaacagc atccgccctt tttccccacc ggaaagcact
    21361 ttagctggct ttttgatgtc gtcctggctg aacagcaaac gaccgagaat gctgcgtacc
    21421 gcctgctcgt catcgccttc ctgcttccac tgactcatcc attcgaacac ggtcagatca
    21481 ttttcaaact catattcgtg gtcctgggcg tagtaaccaa tgcgcgcgtt ttcagaccat
    21541 tttacggtgc cgctgtccgg ttgcagatcg cccaccagcg ttttcagcag cgttgattta
    21601 ccgacgccgt tggtacccag taccgcaagt ttttcaccga cctccagcag caggttgaga
    21661 tttttaaaca gtggaccgtt atcaacccct ttggtcagac cttccacttc cagcgcgtta
    21721 cggaacagtt tcttatcctg ttcaaaacgg atgaacgggt tctgacggct ggaggctttc
    21781 acctcttcca gtttgatttt atcaatctgg cgcgcgcgcg aagttgcctg gcgagatttc
    21841 gaggcgttgg cgctaaagcg gctaacgaaa gattgcaact cagcaatctg cgctttcttc
    21901 ttggcgttat cggccagcag acgttcacgc gcctgggtcg ccgccgtcat gtactcatcg
    21961 tagttacccg gataaacgcg cagctcgccg taatccagat ccgccatgtg ggtacagacc
    22021 atgttaagga agtgacggtc gtgcgagatg atgatcatgg tgctgtcacg ctcgttcagc
    22081 acctgttcca gccagcgaat ggtgtcgatg tcgaggttgt tggtcggttc gtcgagcagg
    22141 agaatatccg gatcagcaaa cagcgcctgc gccagaagca cacgcagttt ccagccagga
    22201 gcaacttcac tcatcgggcc gtagtgctgt tccactggaa ttcccacgcc aagcaacagt
    22261 tcaccggcgc gagcttccgc agagtaaccg tccatttcgc cgtatttaac ttccagatcg
    22321 gccactttat agccgtcttc ctcactcatt tccggcaaag catagatgcg gtcacgctcc
    22381 tgcttcactt cccacaactc tttatgcccc atgatcaccg tatccagcac agtgaactct
    22441 tcaaaggcaa actgatcctg acgcagctta ccaatgcgct cgttgggatc gagggaaacg
    22501 ttacccaaag tcggctctaa gtcgccgccg aggatcttca taaaggtgga tttaccacta
    22561 ccgttcgcgc caatcaggcc gtaacggttg ccgccgccga atttgacgga aatgttttca
    22621 aacaacggct tactgccgaa ctgcatggtg acgttactgg aaactaacac aggcgtatcc
    22681 tgaaaagaga tatgacaaac cgcgcattat gccataagtc tgaaagaaga tcctgctcct
    22741 cgcttaaggt ctacactcaa gccaaaggca aaaaatgtga tttcgtacac atctgatttc
    22801 actgtgagct ggaatgaact tataatgcgc ttccaatact ctaatattct caacccaatg
    22861 gcctgccagg cacaaaatct cgcttaacat gaatatgaaa ttgaaaacat tattcgcagc
    22921 ggccttcgct gttgtcggct tttgcagtac cgcctctgcg gtaacttatc ctctgccaac
    22981 cgacgggagt cgcctggttg gtcagaatca ggtgatcacc attcctgaag gtaacactca
    23041 gccgctggag tattttgccg cggagtacca gatggggctt tccaatatga tggaagcgaa
    23101 cccgggtgtg gataccttcc tgccgaaagg cggtactgta ctgaacattc cgcagcagct
    23161 gatcctgccg gataccgttc atgaaggcat cgtcattaac agtgcagaga tgcgtctgta
    23221 ttactatccg aaagggacca acaccgttat cgtgctgccg atcggcattg gtcagttagg
    23281 caaagatacg cctatcaact ggaccaccaa agttgagcgt aagaaagcag gcccgacctg
    23341 gacgccgacc gccaaaatgc acgcagagta ccgcgctgcg ggcgaaccgc ttccggctgt
    23401 cgttccggca ggtccggata acccgatggg gctgtatgca ctctacatcg gtcgcctgta
    23461 cgctatccat ggcaccaacg ccaacttcgg tatcggcctg cgtgtaagtc atggttgtgt
    23521 gcgtctgcgt aacgaagaca tcaaattcct gttcgagaaa gtaccggtcg gtacccgcgt
    23581 acagtttatt gatgagccgg taaaagcgac caccgagcca gacggcagcc gttatattga
    23641 agtccataac ccgctgtcta ccaccgaagc ccagtttgaa ggtcaggaaa ttgtgccaat
    23701 taccctgacg aagagcgtgc aaacagtgac cggtcagcca gatgttgacc aggttgttct
    23761 tgatgaagcg attaaaaacc gctccgggat gccggttcgt ctgaattaat cttcaaaact
    23821 taaagcaaaa ggcggactca taatccgcct tttttatttg ccagacccta gttggccggg
    23881 agtataacta acaaaacata tcccaccaac gccgcccaca acagcatcgg tattgaatag
    23941 agatggaaac gccaccagat gcggcgatcg ttcgccatac gtagcgcaat caaatttgcc
    24001 agcgatccgg gtaataaccc aaagccacct acgtttaccg cccataccag taataaagac
    24061 ggcggcacat agttcagcaa caatatggta ctcggcacgt tactgatcac ctgcgataaa
    24121 ccgattgccg ttaaccataa cccgggttca gatagatgac tcacgttacc caacacgcct
    24181 tgcaacgctg gaagctgggt cagtaaatgg acgtcgataa acatcgccat aaacaccagc
    24241 agcagcgtcc agtccacact gagcaccacg cgacgtgcca gcagcgcaaa gcctgccgcc
    24301 acaatcacca gtccccacag ctcttgtttg aactccagcg ccgtcagaaa gacgatatac
    24361 agccccagac aactccacac cagccgcggt ttccactccg gtgtttgcac ccccgtatgg
    24421 tattgcagcg cctttccagg gaaacaacac cagcacagga gcaggagcgt cagcatcatt
    24481 gcgccagcca gcggtgccat ttgggcaata aatccggcaa acgaaagacc agaacgtccc
    24541 cagataagaa tattttgcgg gttgccaatt ggcgtcagta gcgaaccagc gttgactgcc
    24601 agcgcctcaa aaataatcag ccgattaacc gggatctcac acagtctttt tagcgtgata
    24661 gtcagcggaa caacaataaa cagcgcgaca tcgttggtca gaaaggtaga aagcagcgcc
    24721 gccgccagca ccataaacat cgccagccga cgctccgtag caaagcggcg caccattttg
    24781 cgccccagca catcaaaata accgcttaac tccacacctt tggtcagcag catcaggccg
    24841 cttaaggtga tgatggtgtg ccagtcgata gcagcaggcc aggatttcgg tgcaaagggc
    24901 acgaaaaagc ttaatccgat accaacaaga attaataact gaaaaaaacg atcgccttgc
    24961 agcgtgcgta aaaaaggcag gctcatttgg caccgtgttt ctgggtaaac aaacgaaaag
    25021 cgtcgagcgt ctcttcacta acatggtgct ccatgccttc cgcgtcgcga cgggcgattt
    25081 ccggactgac gcccaacacc aacaagaaat tttcgactat ctgatgtcgc tcgcggcttt
    25141 cctgcgccag cttctctcct tctgccgtta aaaacacgcc acgccagggg atcatttcaa
    25201 tcagccccat ggttgccagc cgcttaagca ttttagccac cgtcggttgc gaaactccca
    25261 gacgagcagc catgtccacc tgacgagctt cccccacttc cctgatcaag tcagaaatca
    25321 gctcaacgta atcatcaata agctcgcgcc gatgcgcctc tctgacctgg cggaaccctt
    25381 caacgtgctc ttccacgttc actaactgcg tcactttttt tgctgttggc gtacctgcgc
    25441 gacgactcat tgtgcttcct cattatggtg acgcatacat agcgttctca tttcgtgcgc
    25501 acattgtaaa ccagagttgc gaaggtacaa aaaattaacg ttttagcaat agctacataa
    25561 tatagcctgt gctatatctg tatgtaatgc aatcatccct caaggatcga cgggattagc
    25621 aagtcaggag gtcttatgaa tgagttcaag aagtgtatgc gcgtgtttag tcattctccc
    25681 tttaaagtac ggttaatgct gctctctatg ttgtgcgata tggtcaacaa caaaccgcag
    25741 caagataaac cttccgataa atagcggcgt cgcggtacgc cgcttcactc ctgcttttat
    25801 gcaggcataa cgcgttttgg tctgaaaaac cccacttttt gtcggatttg caatcccctt
    25861 cgcaaaagat ttgttcgtca gtagttgacc tgaacggcgg ctcgctctat cttcttgcag
    25921 ccctgcgtat attgcggctc gcggatgcgg acccctttcc cctcttcacg cactcttgca
    25981 ggtattgacc cttgacgcca gggtaagcac atggcgtttg ttacgatagt ggcatatgaa
    26041 tttaaccctc aaagaatcgc ttgttacccg tagccgggta tttagcccgt ggactgcgtt
    26101 ctacttttta cagtcgctat taattaacct cggcttaggt taccccttca gtttgctcta
    26161 caccgctgcg tttacggcta ttttgctttt gctatggcga acattgcctc gcgtacaaaa
    26221 agttctggtc ggtgtcagtt cgctggtggc ggcttgttat ttcccttttg ctcaggccta
    26281 cggcgcgcct aatttcaata cattgctggc attgcactcc accaatatgg aagagtcgac
    26341 cgaaatcctg acgatttttc cgtggtacag ctacctggtc ggcttattta tttttgcgct
    26401 cggcgtaata gcaatcaggc gaaaaaaaga gaatgaaaaa gcgcgctgga ataccttcga
    26461 cagcctgtgt ctggtattca gtgtggcgac attttttgtt gctcccgtgc aaaacctggc
    26521 ctggggtggc gtatttaaac tgaaagatac tggctatccg gtatttcgtt ttgctaagga
    26581 tgtcatcgtc aataataacg aggtgattga agagcaagaa cggatggcaa aactttccgg
    26641 aatgaaagat acctggacgg tcactgccgt taagccgaag tatcagacct atgtggtggt
    26701 gatcggcgaa agcgcgcgtc gcgatgccct cggtgccttt ggcggtcact gggacaatac
    26761 cccgtttgcc agcagcgtta acggtttgat atttgctgac tacattgccg ccagtggctc
    26821 cacgcagaaa tcacttggct taacgctcaa tcgcgtagtc gatggcaaac cacagtttca
    26881 ggataacttt gtcaccctgg caaatcgcgc gggcttccag acctggtggt tttccaacca
    26941 gggccaaatc ggcgaatacg atactgctat cgccagcatc gccaaacgtg cagatgaagt
    27001 gtacttcctg aaagaaggta attttgaagc agataaaaac acgaaagacg aagcgttact
    27061 ggatatgacc gctcaagtgc tggcgcaaga gcactcgcaa ccgcagctga ttgttctgca
    27121 tctgatgggc tcgcatccgc aggcctgcga caggacacaa ggcaaatacg aaacctttgt
    27181 gcaatcgaaa gaaacgtcgt gctatctcta taccatgacg caaacggacg atttactgcg
    27241 caagctgtac gatcagttac gcaacagcgg cagcagcttc tctctggttt acttttctga
    27301 ccacggtctg gcctttaaag agcgcggcaa agacgtgcaa taccttgccc atgatgataa
    27361 atatcagcaa aatttccagg tgccttttat ggtcatttcc agcgacgata aagcgcatcg
    27421 ggtgattaaa gcccgccgct cagccaatga cttcttaggc tttttctcgc agtggacggg
    27481 aattaaagcg aaggaaataa atatcaaata cccgtttata tctgagaaga aagccgggcc
    27541 gatatacatc accaacttcc agttacagaa ggtggattac aaccatctcg gaaccgatat
    27601 tttcgacccg aaaccttaaa acaaaaaatc cgccccgaga ggcggatttt ttatatcacc
    27661 aaagtgatta gaagcggtaa ccaacaccgg caatccaggt gcctacgtca acgctacgaa
    27721 tacggctctg ctcgtaagag aagtccagag caacgttttc catcgggttg aactgcagac
    27781 cagcaccgta ggagaaaccg tagtcgctgg tgtcgtgttt gtaggtcggg tattcagtgg
    27841 tctggaattt accataaccc acccccacta caccgtagat gcttgcccag tcgttaatgc
    27901 ggtaagccgg accagcagtg atgccgtagt actggttttt gttgtagtca ccagagcttg
    27961 cagtacggct tttctcggtg taagtgaaag aaccgatcac acccagcggg ctgttgtctt
    28021 cttcatagcg gtatttcagg ttgaaaccgc ccattttgtt catttggccc tgagcgtcgc
    28081 tctgtgcgta accgccagtt acggtagaag tcgcagctac ggaagtacct gcggtgaaag
    28141 ccagaactgc ggccagtgct gaaagacatg caattttttt cataaccacc tcaaatgtga
    28201 ttcaaataag tcctaagttt taaatatatc aaaaattaat gggaaactct tcgcgatttg
    28261 tgatgtctaa cgggccattt catgtaacag aacgtttcca tacaccgcta tccatctaaa
    28321 tttaaatcac tttttcagag aactgcgtaa gtattacgca tgttttccct gtcattcatc
    28381 cagattattc ctaatcacca gactaatgat tccatcaatc ctggcgcatt ttagtcaaaa
    28441 cgggggaaaa ttttttcaac aaatgctcaa ccagcattgg gtatatccag tacactccac
    28501 gctttactta agtctagata tttgtgggag aaaggatgcc tggttcatta cgtaaaatgc
    28561 cggtctggtt accaatagtc atattgctcg ttgccatggc gtctattcag ggtggagcct
    28621 cgttagctaa gtcacttttt cctctggtgg gcgcaccggg tgtcactgcg ctgcgtctgg
    28681 cattaggaac gctgatcctc atcgcgttct ttaagccatg gcgactgcgc tttgccaaag
    28741 agcaacggtt accgctgttg ttttacggcg tttcgctggg tgggatgaat tatctttttt
    28801 atctttctat tcagacagta ccgctgggta ttgcggtggc gctggagttc accggaccac
    28861 tggcggtggc gctgttctct tctcgtcgcc cggtagattt cgtctgggtt gtgctggcgg
    28921 ttcttggtct gtggttcctg ctaccgctgg ggcaagacgt ttcccatgtc gatttaaccg
    28981 gctgtgcgct ggcactgggg gccggggctt gttgggctat ttacatttta agtgggcaac
    29041 gcgcaggagc ggaacatggc cctgcgacgg tggcaattgg ttcgttgatt gcagcgttaa
    29101 ttttcgtgcc aattggagcg cttcaggctg gtgaagcact ctggcactgg tcggttattc
    29161 cattgggtct ggctgtcgct attctctcga ccgctctgcc ttattcgctg gaaatgattg
    29221 ccctcacccg tttgccaaca cggacatttg gtacgctgat gagcatggaa ccggcgctgg
    29281 ctgccgtttc cgggatgatt ttcctcggag aaacactgac acccatacag ctgctggcgc
    29341 tcggcgctat catcgccgct tcaatggggt ctacgctgac agtacgcaaa gagagcaaaa
    29401 taaaagaatt agacattaat taaatttaca tttctgcatg gttatgcata accatgcaga
    29461 atttctcgct acttttcctc tacaccgtct ttatatatcg aattatgcaa aagcatattt
    29521 attccgaaaa ttcctggcga gcagataaat aagaattgtt cttatcaata tatctaactc
    29581 attgaatctt tattagtttt gtttttcacg cttgttacca ctattagtgt gataggaaca
    29641 gccagaatag cggaacacat agccggtgct atacttagtc tcgttaatta ctgggacata
    29701 acatcaagag gatatgaaat tatgagtacc gctaaattag ttaaatcaaa agcgaccaat
    29761 ctgctttata cccgcaacga tgtctccgac agcgagaaaa aagcaacagt agagttgctg
    29821 aatcgccagg ttatccagtt tattgatctt tctttgatta ccaaacaagc gcactggaac
    29881 atgcgcggcg ctaacttcat tgccgtacat gaaatgctgg atggcttccg caccgcactg
    29941 atcgatcatc tggataccat ggcagaacgt gcagtgcagc tgggcggtgt agctctgggg
    30001 accactcaag ttatcaacag caaaaccccg ctgaaaagtt acccgctgga catccacaac
    30061 gttcaggatc acctgaaaga actggctgac cgttacgcaa tcgtcgctaa tgacgtacgc
    30121 aaagcgattg gcgaagcgaa agatgacgac accgcagata tcctgaccgc cgcgtctcgc
    30181 gacctggata aattcctgtg gtttatcgag tctaacatcg aataaatcca tcgctgatgg
    30241 tgcagaactt tagtacccga taaaagcggc ttcctgacag gaggccgttt tgttttgcag
    30301 cccacctcaa cgcacttatt tagtgcatcc atctgctatc tccagctgat taagtaaatt
    30361 ttttgtatcc acatcatcac acaatcgtta cataaagatt gttttttcat caggttttac
    30421 gctaaataat cactgtgttg agtgcacaat tttagcgcac cagattggtg ccccagaatg
    30481 gtgcatcttc agggtattgc cctataaatc gtgcatcacg tttttgccgc atctcgaaaa
    30541 atcaaggagt tgcaaaactg gcacgatttt ttcatatatg tgaatgtcac gcaggggatc
    30601 gtcccgtgga tagaaaaaag gaaatgctat gaagtctgta ttaaaagttt cactggctgc
    30661 actgaccctg gcttttgcgg tttcttctca tgccgcggat aaaaaattag ttgtcgcgac
    30721 ggataccgcc ttcgttccgt ttgaatttaa acagggcgat aaatatgtgg gctttgacgt
    30781 tgatctgtgg gctgccatcg ctaaagagct gaagctggat tacgaactga agccgatgga
    30841 tttcagtggg atcattccgg cactgcaaac caaaaacgtc gatctggcgc tggcgggcat
    30901 taccatcacc gacgagcgta aaaaagcgat cgatttctct gacggctact acaaaagcgg
    30961 cctgttagtg atggtgaaag ctaacaataa cgatgtgaaa agcgtgaaag atctcgacgg
    31021 gaaagtggtt gctgttaaga gcggtactgg ctccgttgat tacgcgaaag caaacatcaa
    31081 aactaaagat ctgcgtcagt tcccgaacat cgataacgcc tatatggaac tgggcaccaa
    31141 ccgcgcagac gccgttctgc acgatacgcc aaacattctg tacttcatca aaaccgccgg
    31201 taacggtcag ttcaaagcgg taggtgactc tctggaagcg cagcaatacg gtattgcgtt
    31261 cccgaaaggt agcgacgagc tgcgtgacaa agtcaacggc gcgttgaaaa ccctgcgcga
    31321 gaacggaact tacaacgaaa tctacaaaaa atggttcggt actgaaccga aataataacg
    31381 ctacacctgt aaagcgcact ggcagttccc tctcccctct ggggagagga ttagggtgag
    31441 gggcgcaaac ccgctccggg gccattaatt accctgaatt tgattattta caccacggta
    31501 acaggaacaa catatgcagt ttgactggag tgccatctgg cctgccattc cgcttctgat
    31561 tgaaggtgcc aaaatgaccc tgtggatttc ggtcctcggt ctggctggcg gtctggtaat
    31621 cggattgctg gcaggttttg cacgcacctt cggtggttgg atagccaacc acgtcgcgct
    31681 ggtctttatt gaggtgatcc gcggcacacc tatcgtcgtc caggtgatgt ttatttattt
    31741 cgccctgccg atggcgttta acgacttacg catcgaccca tttactgcgg cggtggtcac
    31801 catcatgatc aactccggcg cgtacattgc ggaaatcacg cgtggtgcgg tgctgtctat
    31861 tcacaaaggt tttcgtgaag caggactggc gctcggtctt tcacgttggg aaaccattcg
    31921 ctacgtcatt ttaccgctgg cactgcgtcg tatgctgccg ccgctgggta accagtggat
    31981 catcagcatt aaagacacct cgctgtttat tgtgatcggc gtggcggaac tgacccgtca
    32041 ggggcaagaa attattgccg gtaacttccg cgcccttgag atctggagcg ccgtggcggt
    32101 gttctatctg attattaccc tggtgctaag ctttattttg cgtcgtctgg aaagaaggat
    32161 gaaaatcctg tgattgaatt taaaaacgtc tccaagcact ttggcccaac ccaggtgctt
    32221 cacaatatcg atttgaacat tgcccagggc gaagtcgtgg tgattatcgg gccgtccggt
    32281 tccggtaaat cgaccctgct gcgctgcatc aacaaactgg aagaaatcac ctccggcgat
    32341 ctgattgtcg atggcctgaa ggttaacgat ccgaaagttg acgagcgcct gattcgccag
    32401 gaagcaggta tggtgttcca gcagttttac ctcttcccgc atctgacggc gctggaaaac
    32461 gtcatgtttg gtccgctacg cgtgcgtggc gcgaacaaag aagaggcgga aaaactggcg
    32521 cgtgagctgc tggcgaaagt cggtctggca gaacgtgcac atcactaccc ttccgaactt
    32581 tctggtggtc aacagcagcg tgtggcgatt gcccgcgcgc tggcggtgaa gccgaaaatg
    32641 atgctgtttg atgaaccgac ttccgctctt gacccggaac tgcgccatga agtgctgaag
    32701 gttatgcagg atctggctga agaagggatg acgatggtga tcgtgaccca cgaaatcggt
    32761 tttgccgaga aagtagcttc gcggctgatc tttatcgaca aaggccggat tgcggaagat
    32821 ggcgatccgc aggtattgat caagaacccg ccgagccagc gcttgcagga atttttgcag
    32881 cacgtctctt aacttctctg cctccctttc acccgaaagg gaggcacacc agattcctct
    32941 catttaaaat cgcccctccc ccagcatcta tacttatctt tttgctctgt tttctcactg
    33001 gaggagtcat gcggtggatc ctgttcatcc tcttctgcct gctgggcgca cctgcccacg
    33061 cggtatccat acccggcgtt acaaccacaa cgacaacgga ctcaacgact gaaccggccc
    33121 cggaaccgga tatcgaacaa aaaaaagcgg cctatggcgc actggcggat gtgctggata
    33181 acgacacctc gcgtaaagaa ttgatcgacc agttgcgtac tgtcgccgcg acgccccctg
    33241 cggaaccggt cccgaagatc gtgccgccga cgctggttga agagcaaacc gtgctgcaaa
    33301 aggtcaccga agtcagccgc cattatggtg aagccctttc cgcccgcttc gggcaacttt
    33361 atcgcaatat caccggctcc ccgcataagc catttaatcc acaaaccttc agcaatgcgc
    33421 tgagccattt ttcaatgtta gcggtattag tgtttggttt ttactggctg attcgcctgt
    33481 gcgcactgcc gctgtatcgc aaaatgggtc agtgggcgag gcaaaaaaat cgtgagcgca
    33541 gcaactggtt gcagcttccg gcgatgatta tcggggcgtt tattatcgac ctgctgttac
    33601 tggccctgac attatttgtc ggccaggtat taagcgacaa cctgaatgca ggcagtcgca
    33661 ccatcgcttt ccaacaaagt ttgtttctca acgcctttgc cctgattgaa tttttcaaag
    33721 ccgtactacg cctgattttt tgcccaaacg tggcggagct gcgcccgttc acgattcagg
    33781 acgagagcgc ccgttactgg agtcgtcgcc tgagctggtt aagcagtttg ataggttatg
    33841 gcctgattgt ggccgtgccg attatctcta atcaggtgaa tgtacagata ggtgcgctgg
    33901 cgaacgtcat cattatgctg tgcatgaccg tctgggcgtt gtacctgatc tttcgtaata
    33961 aaaaagagat tacccagcat ttgctcaact ttgcggagca ttcgctggcc tttttcagcc
    34021 tgtttatccg cgcctttgcg ctggtgtggc actggctggc aagcgcctat tttatcgtgc
    34081 tgtttttctt ttcgttgttc gatccgggca acagcctgaa atttatgatg ggtgcaacgg
    34141 tgcgcagcct ggcgattatt ggtatcgcag cgtttgtttc cggtatgttt tcccgctggc
    34201 tggcaaaaac catcactctc tcgccacata ctcagcgtaa ctatccagag ttgcaaaaac
    34261 ggctgaatgg ctggctttcg gcggcgctga aaacggcgcg tattctgacc gtctgcgtgg
    34321 cggtaatgtt gctgttgagc gcatggggat tgttcgattt ctggaactgg ctgcaaaacg
    34381 gcgcggggca gaaaaccgta gatatcctga tccgtatcgc acttattctt tttttctcgg
    34441 cggttggctg gacggtgctc gccagtttga tcgaaaaccg gctggcttcg gatattcatg
    34501 gccgcccgct acccagcgcc cgtacgcgta ccctgctgac gctgttccgt aacgcgctgg
    34561 cggtgattat cagtaccatc accatcatga ttgtgttgtc ggaaatcggc gtcaatatcg
    34621 cgccattgct ggcaggtgcc ggggcattag gtctggctat ctcgtttggt tcgcaaacgc
    34681 tggtgaaaga tatcattacc ggggtgttta ttcagtttga aaacggcatg aacactggag
    34741 atttggtgac tatcgggccg ttgaccggca cagtggaacg gatgtcgatt cgctccgtgg
    34801 gcgtgcgcca ggataccggg gcgtatcaca tcattccgtg gtcttcgata accacctttg
    34861 ctaacttcgt ccgcggtatt ggttcggtag tggcaaacta cgatgttgat cgccatgaag
    34921 atgctgataa agccaatcag gcactgaaag atgcggtagc ggaattaatg gaaaacgaag
    34981 aaattcgcgg gctgattatt ggtgaaccga attttgccgg gattgtcggc ttaagcaata
    35041 ccgcgtttac actgcgtgtt tcgttcacca cgctgccact caaacagtgg acggtacgct
    35101 ttgccctcga cagccaggtg aaaaaacatt tcgacctggc gggcgttcgc gcgccagtgc
    35161 agacttatca ggtgctgcct actccgggcg cgaccccggc tgaaccgtta ccgccggggg
    35221 agccaacgct ttaacgctgg cgattgacaa aacggcggcg ctgctcgtcg ttcataaagg
    35281 tccaggcaat aaagcgactc tgcttttgcc cctgggccat ctcttttttt accaccttca
    35341 ccgcgcccac gtcggtcaga gcacgataca acggcggtaa gttttcacca cgagatacca
    35401 gcgaggtaaa ccacatcacc tgcttcgcga agcctttgct ctcttcaatc atctttttga
    35461 taaaggcgac ttcaccacct tcacaccaca actcctgttg ctgaccacca aagttcagtg
    35521 catcgtcttt gttcagcccc aggttacggc gtttacgctc actacctgcc cgcgctgcag
    35581 cggcggaatc gtggaatggc gggttacaca aggtcgcgtc gtattgctcg tttttatgga
    35641 tgatgccgtt aaaaattgcc ccactctctt tttgccgacg cagacgaatg gcgcggttaa
    35701 gccccggatt agcactgata atcgcctgcg cactgcttaa cgcctggctg ctggtttcac
    35761 taccggtaaa acgccagcca tattcatgta cgccaatcag cggataaata cagttcgcac
    35821 caacgccgat atccagaatg ctggcattcg ctggaattgt tccgcttgct tcagccagta
    35881 aatcggcaag gtgatgaata taatccgccc ggcccggtac tggtgggcag agaaaaccgt
    35941 cggggatatc ccagttcgct acggcgtaaa aatgggccag caacgcctta ttgagcgcct
    36001 tcaccgccag cggattggca aagtctacgc tttgctcccc ggcgggtgta agcgtgagga
    36061 attgcctgag ttcaggattg acctgacaaa gcgtggcgag atcgtagcgg ctgtgatgac
    36121 ggttgcgcgg atgcaacccc ggtttctggg cggacatagc attctccttt aagcagcggc
    36181 gtaagatacc cgcataacgc gacagggtaa atatctggct gcgtttatca ttccccaaaa
    36241 ccttaacatg gctatggttt atggcaattc atcataaaaa tcagcattat gcatgtcatt
    36301 aatttgtctt attttgtaaa aatatatcgt tcaaaaaata agcacatctc tgtcaatttt
    36361 ttgcgtcatt tgcatcgcgc aggcgaccta cacttatcag gcactacccg ttgtatttat
    36421 gaggatgtca ttatgaaaaa gtgcctcaca ctactgattg ccaccgtcct gagcggaatc
    36481 tctctcacgg cttatgccgc gcaaccgatg agtaacctgg acagcggtca gctacggccc
    36541 gccggtaccg tttcggcaac tggcgcatca aacctaagcg atctggagga taaactggca
    36601 gaaaaagcgc gcgaacaagg cgcgaaaggt tatgtcatta attccgcagg cggaaatgac
    36661 cagatgttcg gtactgcaac catctacaaa taaccccacc aacgtaactg cattcgcctc
    36721 tggatgcagt tttcgcctgt tttgtaatca aaaaacatca aatatgaact caatgtaaat
    36781 aaatgtattt ctttttcgcg caatgggtga tagaaaatcg cgccaagtga taatgcttat
    36841 caaaattatt atcacttgca cgaacactat cacgggatta acagtggcat cgcatccgca
    36901 gagatgcttt ctcgtggcag tgaaaatttc aacatataag aaaaagtcac ctgcaaaatg
    36961 gaaaacaatc gcaatttccc tgccagacaa tttcattcgc tcacgttctt tgccggtctt
    37021 tgtattggca tcacgcctgt ggctcaggca ctcgccgccg aagggcaaac taacgcggat
    37081 gacacgctgg ttgtcgaagc atcaacgcct tcgctttatg cgccacaaca atctgccgat
    37141 ccgaaattct cgcgtccggt agcggatact acccgcacga tgacggtgat ttctgaacaa
    37201 gtgattaaag atcagggcgc aaccaacctt accgacgcgc tcaaaaacgc ccccggcg
//
LOCUS       DAESHI010000034        35778 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.034, whole genome shotgun sequence.
ACCESSION   DAESHI010000034 DAESHI010000000
VERSION     DAESHI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35778
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.034"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(1..581)
                     /locus_tag="KW011_001727"
                     /pseudo
     CDS             complement(1..581)
                     /locus_tag="KW011_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(810..1730)
                     /gene="malM"
                     /locus_tag="KW011_001728"
     CDS             complement(810..1730)
                     /gene="malM"
                     /locus_tag="KW011_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HBH7660082.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="KW011_001729"
     CDS             complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="KW011_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HBH7660083.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(3294..4409)
                     /gene="malK"
                     /locus_tag="KW011_001730"
     CDS             complement(3294..4409)
                     /gene="malK"
                     /locus_tag="KW011_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HBH7660084.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            4774..5964
                     /gene="malE"
                     /locus_tag="KW011_001731"
     CDS             4774..5964
                     /gene="malE"
                     /locus_tag="KW011_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HBH7660085.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            6118..7662
                     /gene="malF"
                     /locus_tag="KW011_001732"
     CDS             6118..7662
                     /gene="malF"
                     /locus_tag="KW011_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HBH7660086.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            7677..8567
                     /gene="malG"
                     /locus_tag="KW011_001733"
     CDS             7677..8567
                     /gene="malG"
                     /locus_tag="KW011_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HBH7660087.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            8939..10414
                     /gene="xylE"
                     /locus_tag="KW011_001734"
     CDS             8939..10414
                     /gene="xylE"
                     /locus_tag="KW011_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HBH7660088.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="KW011_001735"
     CDS             complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="KW011_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HBH7660089.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(10994..11137)
                     /locus_tag="KW011_001736"
     CDS             complement(10994..11137)
                     /locus_tag="KW011_001736"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660090.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            11138..11416
                     /locus_tag="KW011_001737"
     CDS             11138..11416
                     /locus_tag="KW011_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660091.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(11463..13559)
                     /locus_tag="KW011_001738"
     CDS             complement(11463..13559)
                     /locus_tag="KW011_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBH7660092.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13559..14296)
                     /locus_tag="KW011_001739"
     CDS             complement(13559..14296)
                     /locus_tag="KW011_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBH7660093.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(14293..14931)
                     /locus_tag="KW011_001740"
     CDS             complement(14293..14931)
                     /locus_tag="KW011_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HBH7660094.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(15045..15287)
                     /locus_tag="KW011_001741"
     CDS             complement(15045..15287)
                     /locus_tag="KW011_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660095.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(15749..17398)
                     /gene="pgi"
                     /locus_tag="KW011_001742"
     CDS             complement(15749..17398)
                     /gene="pgi"
                     /locus_tag="KW011_001742"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBH7660096.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            17923..19272
                     /gene="lysC"
                     /locus_tag="KW011_001743"
     CDS             17923..19272
                     /gene="lysC"
                     /locus_tag="KW011_001743"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HBH7660097.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            19524..19796
                     /locus_tag="KW011_001744"
     CDS             19524..19796
                     /locus_tag="KW011_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HBH7660098.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(19929..20801)
                     /gene="rluF"
                     /locus_tag="KW011_001745"
     CDS             complement(19929..20801)
                     /gene="rluF"
                     /locus_tag="KW011_001745"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HBH7660099.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            21149..21986
                     /locus_tag="KW011_001746"
                     /pseudo
     CDS             21149..21986
                     /locus_tag="KW011_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            22056..22862
                     /locus_tag="KW011_001747"
     CDS             22056..22862
                     /locus_tag="KW011_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBH7660100.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            22872..23279
                     /locus_tag="KW011_001748"
     CDS             22872..23279
                     /locus_tag="KW011_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HBH7660101.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            23279..23773
                     /locus_tag="KW011_001749"
                     /pseudo
     CDS             23279..23773
                     /locus_tag="KW011_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            23839..24636
                     /locus_tag="KW011_001750"
     CDS             23839..24636
                     /locus_tag="KW011_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBH7660102.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            24647..25471
                     /locus_tag="KW011_001751"
     CDS             24647..25471
                     /locus_tag="KW011_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBH7660103.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            25523..26751
                     /gene="sorE"
                     /locus_tag="KW011_001752"
                     /pseudo
     CDS             25523..26751
                     /gene="sorE"
                     /locus_tag="KW011_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            27134..27823
                     /gene="pepE"
                     /locus_tag="KW011_001753"
     CDS             27134..27823
                     /gene="pepE"
                     /locus_tag="KW011_001753"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HBH7660104.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(27914..29545)
                     /locus_tag="KW011_001754"
     CDS             complement(27914..29545)
                     /locus_tag="KW011_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBH7660105.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(29765..33448)
                     /gene="metH"
                     /locus_tag="KW011_001755"
     CDS             complement(29765..33448)
                     /gene="metH"
                     /locus_tag="KW011_001755"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBH7660106.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            33648..34472
                     /gene="iclR"
                     /locus_tag="KW011_001756"
     CDS             33648..34472
                     /gene="iclR"
                     /locus_tag="KW011_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HBH7660107.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            34789..35778
                     /gene="espL4"
                     /locus_tag="KW011_001757"
                     /pseudo
     CDS             34789..35778
                     /gene="espL4"
                     /locus_tag="KW011_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
BASE COUNT         8595 a         9228 c         9105 g         8850 t
ORIGIN      
        1 actatgacca agttgactct gcgagaagtc aactgtatca agaatatttt ggctaaaatc
       61 aacacattcg ccactcgttt tgagaaaaat cacattgtgt aaacgtcgac atttaaatat
      121 gacattatca actgttgcct tagtaaaatt gcaattctca atttctttac attcgaaagt
      181 tacatgttgt agatttgatg ataaaaaaga caccttagaa ataacgtcac ctttaaaatg
      241 acaatttatt aaatcaaaat cagacaggtc gatatacccc tgcgcatctt tatccatcag
      301 aaaattacaa gcgcattcta ttttttcatt tctgtagaat tttacactta tagtatcatt
      361 actaattgag acttcacgta acatatttat tgcatgattt tttcgttccg ttgttttata
      421 tttaaaaata tatcactgat ataaataaag ttatctctgt ttaaacctgt caccccattg
      481 aaattacgta gtaaatcaag atctactata ttatttcctt gaaccacatc atgtttcatc
      541 aaagagttat tgccgccaga atttgtgata tgacctatca taaaacctct gtaatacaga
      601 aataaaataa cctggaaaag tagcctgagc gtccagacac taccggcgaa aggaaaagaa
      661 tctctgataa ggcattgaga taatggacat tcttagtgaa gaagaagtga agtactataa
      721 aaaatcgctc atatcccagg aaggaagcat aaaaaaaggt gcgccaggag acgcaccagt
      781 tgttgcaaaa tcagcactgt ggggcgtaat taccccttgc cttttacact gctgataaag
      841 gtggaacggg cagatgtcga tcccaggcgt tcagcttcat caagcagttt taacgcctta
      901 tcaacatcac ctttcgcgac agcgtttttg atcgcggtat taaaataact ttccgtgtcg
      961 ttgagcatcg gctcgctttt cttcaccggc accggagcag gtgcagccac agctggtgcc
     1021 gccgtgttgc ctaccgtaac cggagctgga gcggaagaac caaatagtgg tcctaccaac
     1081 acgctggagc tggagttcgt tttgactttc agtttcagta agccatcgct ggtatgacga
     1141 gcaaccggat cgggaatatc cgggatcgag ttaccgacgc ctttggcata ggctttagcc
     1201 gggtcgagca gttgggtcgt ctgctggaga tctttttccg tggtaaagac cagaacataa
     1261 agtttttgct gccccaacgc cggtgtcagg cgcataacgc cttccagccg atctgcactc
     1321 atcacgcctg gttcctggta ggtgaaataa ctgctgggga agaaggctga tggggtcatg
     1381 ttctgatcaa gaatcagcac gttcggcgca aaaacgctgg tttgtttgtt cacttcgctg
     1441 gtcagcgtca gggtcagttc gccaatgttt gccgggacgc tgtacgcagc aaccggacca
     1501 ctgatgccgg gaacgttcag ttgttggccg ccggtcgcca gttgggtggt ctgggtttta
     1561 gattgatcga ccggtgtcca ggtgagttgt tgcagcgcgg cagatggaat ggctggcgcg
     1621 tcgctggtgt tttgcggtac gtagttaaca tcggcaaggc taattccagg cgcgcttgcc
     1681 agtaaccctg ctgataaaca gagggcgatg agacttttat tcattttcat tgttttcacc
     1741 tcaaaatctg gagctcagcg gtagccaggc aatagcgcgc taaacccgat aatcagaggg
     1801 gctttcgccc ctttcagata atgacaacct gtttttatgc cggatgcggc gtaaacgcct
     1861 tatccggccc aggttttgct attaccacca gatttccatc tgggcaccga aggtccactc
     1921 gtcgctgtcg ccacgaccga agctgccgcc gttgaaatca gcaggaacgg ctttgccgta
     1981 gttcgggtta accttgctat cgccgttgta gtcgtaaccc catttctcat cccacttggc
     2041 gtaggttgcg aagacacgaa tagccgggcg tgaccagatg ctgtcgccag cctgccattg
     2101 ttgtgcgagg gtaattttgt actgattgtt cttgtcgccg gtgcgctggg attcgacgtt
     2161 gtcgtagccg atttccatca cggtgctcat gattggcgtc cacttgtaca tcgggcgaat
     2221 accgacggtc caccacttgg tgccgttgtc gttatcccag ttgatatcct ggtacatacc
     2281 cacgtacatc atgtcccagt tgtcgcccat ggagatcgca ccgtggtcga ggatacgcag
     2341 catgtgaccg ttgttgttga tattgtaggc aaatttttcg ttatcaaacg cgacgccaga
     2401 accctgcgac agacctttac cctgcgaggt catcgagtca gtagcgtact gaacaacaaa
     2461 cttgttaaag cccttcagga cactctgagt atgttcagca gtgaataacc agccgtcttt
     2521 cgatgcgcca tcaaccagac gatagttatc acgcaggttg gcacgaccgt agtcgacacc
     2581 cagttctaat gtgccgcccg ggttgatttc catctgcgct aaacgcacat cgaaaacgtc
     2641 gttcgcggtt tcgttggtat agtcataaat attgttgctg gcaaaagagg aagaaccacc
     2701 agcttcagag gagcgggttg ctgccagaga gagtttaccg aagccaacat cgatgttttc
     2761 cagaccggca ccaggaccag aaatatccca gtagtagaag tcgatcatat gaacgtcatg
     2821 acgttggtag aagcgcttac ctgcccagat ggtggaacct ggcagccatt cgatcaggtt
     2881 tttaccctgc acgtttgctt cacggaaggc cggatcggta gcttcccagt cattctgttg
     2941 cgcgacggaa taggccacgt tagtgtcgaa atagaagctc ttatcgccct ctttccacac
     3001 ttcctgaccc aattttaatt cagcataagt ttcacattcg ttgccaagac ggtatttact
     3061 ttgagcaccg gtagtctgga aacactgttg ttcaccgccg ctacctgtcc agccaatacc
     3121 ggaacgtgca tagccgtgga aatcaacagc cattgcctga gcagacatta cgcccgctgc
     3181 gacggcaacc gccagaggaa gtttgcgcag agtaatcatc attctatctc ctgagtcatt
     3241 gcttttcttt tttcacatca cctgtgacag gctttgtgtg ttttgtgggg tgcttaaacg
     3301 cccggctcct tatgcagtcg acgacatgca gtgccatcct cacggaacag atggcaacgc
     3361 tctggcggca ggccgatagc gaatgtggca ccttcttcta ccaacaccac gtcgttctgg
     3421 cggtacacca ggttttgacg aatggaaggg atctggatat ggatttgggt ttcgttgccg
     3481 agttgctcga cgacctgaac ttcaccctca aggatgacgt cagcgatatc actcggcagt
     3541 agatgttccg ggcgaatacc cagcgacata ttggctccaa cctggacatc acggctttca
     3601 actggcagcc agacttgctg acgatttggc atcggcagct ccacctgcac ttgatcgatt
     3661 gcggtggcgg tcacttttac cggcaggaag ttcatctttg gcgaaccgat aaatccggcg
     3721 acaaaacggt ctgccggata gtggtacagc tccagcggtt tcccaacctg cgccacgcga
     3781 ccggcgtcca gcaccacgat tttgtcggcc agcgtcatcg cttcgacctg atcgtgggtg
     3841 acgtaaatca ttgtgcggcc caggcgttta tgcagacggg agatttcgat acgcatttgc
     3901 acacgcagtg cagcatcgag gttggagagc ggttcatcga gcaaaaatac gcttggctcg
     3961 gccaccagcg tacggccaat cgccacacgc tgacgctgac caccggagag cgctttcggt
     4021 ttgcgatcca gcaaatgcgc cagttgcagc acttccgcca cctggttgac gcgttggtta
     4081 atcacctctt tttttgcgcc agccagtttc aggccaaatg acatgttttc tgctactgac
     4141 aggtggggat agagcgcgta agactgaaac accataccaa cgccgcgttc tgctggcgga
     4201 gtgtcattca tccgtttctc accgatgaac aggtcgccgc tggtgatcgt ctcaagcccg
     4261 gcaatcatgc gcagtaaagt cgatttaccg cagccagacg gtccgacaaa caccacgaat
     4321 tcaccttcat ggatatcgag attgatatct ttcgatacca cgacctcgcc ccaggctttc
     4381 gttacatttt gcagctgtac gctcgccatg cccttctccc tttgtaacaa cctgtcatcg
     4441 acagcaacat tcatgatggg ctgactatgc gtcatcagga gatggcttaa atcctccacc
     4501 ccctggcttt tttatggggg aggaggcggg aggatgagaa cacggcttct gtgaactaaa
     4561 ccgaggtcat gtaaggaatt tcgtgatgtt gcttgcaaaa atcgtggcga ttttatgtgc
     4621 gcatctccac attaccgcca attctgtaac agagatcaca caaagcaacg gtggggcgta
     4681 ggggcaagga ggatggaaag aggttgccgt ataaagaaac tagagtccgt ttaggtgttt
     4741 tcacgagcac ttcaccaaca aggaccatag attatgaaaa taaaaacagg tgcacgcatc
     4801 ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc caaaatcgaa
     4861 gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc
     4921 ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg
     4981 gaagagaaat tccctcaggt tgcggcaact ggcgatggcc ctgacattat cttctgggcg
     5041 cacgaccgtt ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa
     5101 gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg
     5161 attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg
     5221 aacccaccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt
     5281 aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct
     5341 gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg atattaaaga cgtggccgtg
     5401 gataacgctg gcgcgaaagc gggtctgacc ttcctggttg accttattaa aaacaaacac
     5461 atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg
     5521 atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt
     5581 gtaacggtac tgccgacctt caagggtcaa ccatctaaac cgttcgttgg cgtgctgagc
     5641 gccggtatta acgccgccag tccgaacaaa gagctggcga aagagttcct cgaaaactat
     5701 ctgctgactg acgaaggtct ggaagcggtc aataaagaca aaccgctggg tgcagtggcg
     5761 ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac
     5821 gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg
     5881 cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac
     5941 gcgcagactc gtatcaccaa gtaatgctgt gaaatgccgg atgcggcgtg aacgccttgt
     6001 ccggcctaca aaactgaaac gcatgtaggc ctgataagac gcatcagtgt cgcatcaggc
     6061 agttgttgtc ggataaggcg tgaaagcctt atccgtcctg gaatgaggaa gaaccccatg
     6121 gatgtcatta aaaagaaaca ttggtggcaa agcgacgcgc tgaaatggtc agtgctaggt
     6181 ctgctcggcc tgctggtggg ttaccttgtt gttttaatgt acgcacaagg ggaatacctg
     6241 ttcgccatta ccacgctgat attgagttca gcggggctgt atattttcgc caatcgtaaa
     6301 gcctacgcct ggcgctatgt ttacccggga atggctggaa tgggattatt cgtcctcttc
     6361 cctctggtct gcaccatcgc cattgccttc accaactaca gcagcactaa ccagctgact
     6421 tttgaatgtg cgcaggaagt gttgttagat cgctcctggc aagcaggcaa aacctataac
     6481 tttggtcttt acccggcggg cgatgagtgg caactggcgc tcagtgacgg cgaaatcggc
     6541 aaaaattacc tctccgacgc gtttaaattt ggcggcgaac aaaaactgca actgaaagaa
     6601 acgaccgccc agcccgaagg cgaacgcgcg aatctacgcg tgattaccca gaatcgtcag
     6661 gcgctgagtg acattaccgc cattctgccg gatggcaaca aagtgatgat gagctccctg
     6721 cgccagtttt ctggcacgca gccgctctac acactcgacg gtgacggcac gttgacgaat
     6781 aatcagagcg gcgtgaaata tcgtccgaat aaccaaattg gcttttacca gtccattacc
     6841 gccgacggca actggggtga tgaaaagcta agccccggtt acaccgtgac tactggctgg
     6901 aaaaacttta cccgcgtctt caccgacgaa ggcattcaga aaccgttcct cgccattttc
     6961 gtctggaccg tggtgttctc gctgatcact gtctttttaa cggtggcggt cggcatggtt
     7021 ctggcatgtc tggtgcagtg ggaagcattg cgcggcaaag cggtctatcg cgttctgctg
     7081 attctgccct acgcggtgcc atcattcatt tcaatcttga ttttcaaagg gttgtttaac
     7141 cagagcttcg gtgaaatcaa catgatgttg agcgcgctgt ttggcgtgaa gcccgcctgg
     7201 ttcagcgatc cgaccaccgc ccgcacgatg ctaattatcg tcaatacctg gctgggttat
     7261 ccgtacatga tgatcctctg catgggcttg ctgaaagcga ttccggacga tttgtatgaa
     7321 gcctcagcaa tggatggcgc aggtccgttc cagaacttct ttaagattac gctgccgctg
     7381 ctgattaaac cgctgacgcc gctgatgatc gccagcttcg cctttaactt taacaacttc
     7441 gtgctgattc aactgttaac caacggcggc ccggatcgtc ttggcacgac cacgccagcc
     7501 ggttataccg acctgcttgt taactacacc taccgtatcg cttttgaagg cggcgggggt
     7561 caggacttcg gtctggctgc agcaattgcc acgctgctct tcctgctggt gggtgcgctg
     7621 gcgatagtga acctgaaagc cacgcgaatg aagtttgatt aagggagata acaaaaatgg
     7681 caatggtcca accgaaatcg caaaaagcac gtttgtttat tactcacctg ctactgctac
     7741 tttttatcgc ggcgattatg ttcccgctgc tgatggtcgt cgctatctca ctgcgccagg
     7801 ggaactttgc gatcggcagc ctgatcccgg agcaaatctc ctgggatcac tggaaactgg
     7861 ctttaggttt tagcgtggaa caggctgatg gtcgcattac gccaccgcca ttcccggtac
     7921 tgctgtggct atggaactcg gtaaaggtcg ccgggatttc cgcgattggc attgtcgcgc
     7981 tctctaccac ctgcgcctac gctttcgccc gtatgcgctt tccaggcaaa gcgacgctgc
     8041 tgaaaggaat gctgattttc cagatgttcc cggcagtact ttcactggtc gcgttgtatg
     8101 cgttgtttga tcgtctgggt gagtacattc cattcattgg cctgaatact cacggcggcg
     8161 taattttcgc gtatctgggt gggattgcgc tgcatgtctg gaccatcaaa ggctatttcg
     8221 aaaccatcga tagttcgctg gaagaagcgg ctgcgctgga tggtgcgaca ccgtggcagg
     8281 ccttccgcct tgtcctgttg ccgctgtcag taccgattct ggcggtggta ttcatcctgt
     8341 cgtttatcgc tgccattact gaagttccgg tcgcgtcgct gttactgcgt gacgtaaaca
     8401 gctacaccct ggccgtgggg atgcagcaat acctcaaccc gcaaaactac ctgtggggtg
     8461 actttgccgc cgctgccgtg atgtctgcat taccgatcac catcgtcttc ttgctggctc
     8521 aacgctggct ggtcaacggc ctgacggcag gtggtgtgaa aggttaaaga tgttgttctg
     8581 ccaatgttat gccgctgcac cctcaactta cgttatccca acttgtgact gttattcggc
     8641 gctccacgga gcgccttttt ttctttcgtc tgcaatctga atcgttcgcc ggttaatatt
     8701 tccatcatag agcttattat ttttacgtta tttgttttcc cacttacgat aattctcttt
     8761 cgtgctctga gtcacggcaa tagtattgtt ttcatcaatt tttgataatt atcacaatta
     8821 agatcacaga aaagacatta cgtaaacgca ttgtaaaaaa tgataattgc cttaactgcc
     8881 tgacaattcc aacatcaatg cactgataaa agatcagaat ggtctaaggc aggtctgaat
     8941 gaatacccag tataattcca gttatatatt ttcgattacc ttagtcgcta cattaggtgg
     9001 tttattattt ggctacgaca ccgccgttat ttccggtact gttgagtcac tcaataccgt
     9061 ctttgttgct ccacaaaact taagtgaatc cgctgccaac tccctgttag gattttgcgt
     9121 agccagcgct ctgattggtt gcatcatcgg cggtgccctc ggtggttatt gcagtaaccg
     9181 cttcggccgt cgtgattcac ttaagattgc tgctgtcctg ttttttattt ctggtgtagg
     9241 ttctgcctgg ccagaacttg gttttacctc tataaacccg gacaacacag tacctattta
     9301 tctggcaggt tatgtcccgg aatttgttat ttatcgtatt attggcggta ttggcgttgg
     9361 tttagcctca atgctttcac caatgtatat tgcggaactg gctccagctc atattcgcgg
     9421 aaaactggtt tcatttaacc agtttgcgat tattttcggg caacttttag tttactgcgt
     9481 aaactatttt attgcccgtt ccggtgatgc cagctggctg aatactgacg gctggcgtta
     9541 tatgtttgcc tcggaatgta tccctgcact gctgttctta atgctgctgt ataccgtgcc
     9601 agaaagtcct cgctggctga tgtcacgcgg caagcaagaa caggcggaaa gtatcctgcg
     9661 caaaattatg ggcaacacgc ttgcaactca ggcagtacag gaaattaaac actccctgga
     9721 tcatggccgc aaaaccggtg gtcgtctgct gatgtttggc gtgggcgtga ttgtcatcgg
     9781 cgttatgctc tccatcttcc agcaatttgt cggcatcaat gtggtgctgt actacgcgcc
     9841 ggaagtgttc aaaacgctgg gggccagcac ggatatcgcg ctgttgcaga ccattattgt
     9901 cggagttatc aacctcacct tcaccgttct ggcaattatg acggtggata aatttggtcg
     9961 taagccactg caaattatcg gcgcactcgg aatggcaatc ggtatgttta gcctcggtac
    10021 cgcgttttac actcaggcac cgggtattgt ggcgctactg tcgatgctgt tctatgttgc
    10081 tgcctttgcc atgtcctggg gtccggtatg ctgggtactg ctgtcggaaa tcttcccgaa
    10141 tgctattcgt ggtaaagcgc tggcaatcgc ggtggcggcc cagtggctgg cgaactactt
    10201 cgtctcctgg accttcccga tgatggacaa aaactcctgg ctggtggccc atttccacaa
    10261 cggtttctcc tactggattt acgggtgtat gggcgttctg gcagcactat ttatgtggaa
    10321 atttgtcccg gaaaccaaag gtaaaaccct tgaggagctg gaagccctct gggaaccgga
    10381 aacgaagaaa acacaacaaa ctgctacgct gtaatcttcc tgtccagcac gccgcgccag
    10441 ttcggcgtgc tttcttttta ctcccgcttc agccgtttcg aattacacag ccacagagtg
    10501 atcaccagta acaggatcgc agccgagtag atcaacacat ccagtggcga tttatgatcg
    10561 acgatgatca agcgcacaat ggcggtgatc ccaatataga caaagtaacg tagcgggaag
    10621 tgaaaaccgg actgaaagta cttcacaatc agcgcgataa attcgaaata gagaaagtaa
    10681 accaccagtc cttctaccag ctcatatttg ctggtttgtt ctggcgcgaa cagcacatct
    10741 gccagatgca ccgtttcttt gccgaggaag acaaccaaaa tcaggccgag gcacagcagg
    10801 ccaagattga gtacggtctg caaaatggtg gagataaact ccacgcgcgg acgagagagt
    10861 gacgtcatag cagtacctcc ttcacagtgg cgagcttcct gtataacgca aaaatgtgac
    10921 ggagatctat attttttgtg tatgttttgt tcaacctgga tttggagcgc gcctccacca
    10981 aagccggata tacctacccg taggccggat aagccgcgcc tggcggcgca tccggcaaga
    11041 caaaccgcac ccaagccggt aattcaaaaa tcacccattc aggtgattcc cagccaccag
    11101 caatgcgcga aaaatcgaca atgttcctct ggaacaaatg aaacgtaacc tgataaaggt
    11161 ggtaaaaatg aagccttatt ttgctgcttt gatgttatca gtctctgttc ttcctgctta
    11221 tgctggtccg ctcggtactg ctgataaagc cgacctaccg caaagcaatg tctccagccc
    11281 aatgatggcc cagtcgctca ggcaaccaga cctgcaaccg atatcgacgg acaggaagac
    11341 agaatgtttt cgcctttaca caccagatcg caaaccgggg gtgaactgcg ttcctgatgg
    11401 ctcaacaggc cattaagaat gagggtgcca gtgattgcga aaactggcac ctttcccatg
    11461 acttagcgga aattgacgct acggtcgctg gtcatatcat acaactggaa cttacgccca
    11521 agttgctgac caccgtcacg cgtcagcggc gtccagccaa tcgccgcacg gctgcgtgtc
    11581 gggccagacg agaagagatc caacggtacc gagacataca cgcctttggt gaagtcccct
    11641 tcgccgtact cctcttttga aacattcgtg atcgtggcat agccacccac caccacgccg
    11701 ctatcaaagc gtttggcgat ctccagcgtg ccgcctttat cccctgccag atactgcccg
    11761 acgctggctt tcactaacac gtcctgggcg aaagatggcg tccagtaggc ggtcagatgt
    11821 ccggttttca cgctgtagtc ggtgaatttc atcatatctt ttgcactacg ccagtcgcgc
    11881 tgtttaacgt agttggcatc cagaccaaac gcccagttgc tgtccagcgg acgatacaac
    11941 acttcagccc ccgcaccgcc aaacatggtt tcgagataac ctccatagac ctgaccgtag
    12001 aagccgttgc ccagatgctg gaagtagttg gcttgcaggt tattcacata ggcatcgttc
    12061 tgcacatact cgcgcacatg ggtacgaacg cgcggtaagt gcgagtcctg tggcggattg
    12121 gtgtagttaa acttgtcgta gttgttggca agatttgcaa acaggctgcc gatggtcagc
    12181 agatggtccg tcagccacaa atctgccgtt cccatcacgc ccagctgata catgtaaaag
    12241 ttttccgggc caccgaccga ctggttcagc accggatcga tatggaaatc aaagcgtgat
    12301 ttgtcgatat accagccctg ctcggtggac tgcggaacca ctggctcgac gcgtttttgc
    12361 gccagcgtcg tttcgtggcc caacggctct ccggcgagat gatttttcag gctggcgaca
    12421 tcggtttccg tcgtcacctg cggcatgtta aggcgatttt ccgtaatgcg gatcgttttg
    12481 atcccatccg gcagatcgtt catcacgatc cgattagcac ggatgatccc ttcgcgcgaa
    12541 tcacgatatt tcacctgctc gccggtaaca tacagcgtat cgcctttcgc ctggatctgc
    12601 ggatcggcaa gtccggcatt gtatttcagc aacgttaact gattcgccac caccgagtgc
    12661 tgcaaaatgg catcctgcgg ctgcggttga tattgcgggc gggcgttatc gatgtaggac
    12721 gggcgcagat cgttaaagtt ggtgcgcagc gtgacgccaa acataaaggt gttgccacgt
    12781 tcatagctaa ggttaacgtc ggcccaatcg gtaacgcgat aaatcgcccc gacgttaaac
    12841 ttactttttt gctccagctt cccggcaaaa tcctgctgat aattattacc ttcatactcc
    12901 agtttcaggc gcagcggttg ccagggcgtc tggtattcca cgccgccaaa cagtgaggcc
    12961 gggccgtgga acatctgact gccgtcgata gatcccgctt gtttgtagct gttatcgcga
    13021 tagcaatatt tatcactggc tgaacagagc ggatttttca cattaccgct ggtgcccaga
    13081 tatccccagc ccaggccgag cgtaaaatcg aacggccccc aggctttgct ggcaacaaga
    13141 tattccgcat caaacagccc cgtaccgccg atatcccgcg caccaaccgc cacttgcggc
    13201 agccagtaac tctcttccca caaacgcagt ttgagatcga aggctttatc tttatacgtt
    13261 tgatcgccag agaacgcttc gacgctgctg tactgccggg tgcgcacgtc ggtgtaacgc
    13321 agcgttgttt ccagccacgg gaagagttgc actgaagctg agtaataacg gtactgatcg
    13381 ttatcgcgat agttcagact caactcccct tcccgtgcca tacgcgcggt gggagtttgt
    13441 aataatccta cgccaccgaa atccgactgc gacggaccaa tgggcgcagg atatgtttcg
    13501 ccgtagcaag ctgtgctaat gcccagcgcc agtaagctaa gcagatgtct ttttttcatt
    13561 attggggtat ccgctgcgtc agagtctgaa gaatgtcggc gttaagggca tcaggcgtct
    13621 cactccagac ggagtctgcg aggccaacat aaataatgct gccgggcatc ggctctacgt
    13681 gacgcttgtt ccagtaagcc accggcgctt tttgcgtgcg tccgtccggg taaacgaccc
    13741 acgcgtagct gcgatccgca ccgctgagca ggctctggtc agagagatag ctcgccacgt
    13801 cgcgacctgg agtgaatggc tgattgccag gatggctgat aagcccgaac aacgtgaccg
    13861 tggacggcgg tggtccgacc cacagcgtgt agttgccctg caacggcggg ttaccgcgtt
    13921 cggcaacgcg gacgatatcg ggatcaagat tgattttttg cctgcctgtc acttccaatg
    13981 cctggatttg ctggcgtaag gcgttaatgg cggcggcatc gtcggtgctg gaatctgcgc
    14041 cctgttctgc cagccgtgtc agcaacgcct gctgctgacg caatgctgcc gccgttgcca
    14101 gctcttcgct aatcaccgca ccaggccacc agctgtttgc cagtcgaggt tgccctacca
    14161 gatcgattaa atgctctgcg cccgttagcg ttttggcctc actgctgcca ttgctgaaca
    14221 ccttgacggt tcctgccgcg aagaccgatg acgctcccac actcaaaagc aacgcgacaa
    14281 tagtttgttt aatcattgtt ttgccgcctt gatgagagtg gttttcaccg ggaaataatc
    14341 cgcgccgaga tactgttccg actggcgaat ttgcccttcg ctgtcgatcc agtagcggtt
    14401 atgccagcgc gcctggtcgg tggagacttc ttcgtccagc acgcgaacca gggtttcatc
    14461 gctgccgacc ttgacggtat cggtgccatc ccatttgaag actgagcgtg cggtggcgta
    14521 gcgtacctgc tggtactcgg tccagcccat cgtgcgcgtc catgttgcac catcaacaat
    14581 ttgtgcgggt ttaatcagcg ggtcggcggc aaggttattc acttcaatca ggttgtcgcc
    14641 gccaagcaaa gtcttcacca gacgaccatg ttgtgtgaca agagtggctt gatcctgagt
    14701 gacccatttt tgttgtccgt cttcagcgaa ggccagcacc acaaacaatt gcggcccgcc
    14761 attaagctgc atgtactggc tggcgtaggg catattttga atatcatcgt ccgtcagctg
    14821 tacgcctggc gtgccgaaca gactgtccca cagtgaattg cccagctctt tagtcgtggc
    14881 tgaacaggcc tgtaataaca ggcagataag aatgagtgca ggtcgcttca cgactcttct
    14941 ccagaggggc agaataacca caccggagtg tggttatggt taatcccttc atatttcaag
    15001 ccacagatgc aatgactcga attattttgg ggatacatac tttattactg ggtactggtg
    15061 gttgtggttg ttgtggtccc ggtattagaa ccatcaccgc caccggttgc cgcgagcgcg
    15121 acacccaccg ccgagcttac ggtgctgacg ctggtcgcgg aggaactccc cgccgaaacc
    15181 gacgttgctg ccgcccctgc cgcttcgccc acctgtacag gtgcagccca cacagaagtc
    15241 gccgcaagcg cagatatggc aaaaatgcca tacagaactt ttttcataac aatttccctt
    15301 cattgaatga atggagattt acccaaaaac atttcgggcg ggatcgagta tacacaacta
    15361 aagcatgcga attacggcgc ggggaattag cgatggtgtt ttaggataat tggaatgaat
    15421 atcaaatata agtttcaact tatatttaaa taacaataaa aatcataaac ttaaaataaa
    15481 aacaaaagta attaatctgc gtattctccg aaataaacaa cgattaaaaa tctattttag
    15541 gattaacctc acggtatgat ttccgttaaa tcacagacaa ggggatttat ctgataaaac
    15601 aatgccgact tattgtcggc attgttttta aggttattta actcctcata ctatgcatta
    15661 atgtcgatat tggcatcagg ctccggttgc cggatgcggc gtaaacgcct tatccggcct
    15721 acatatcggc atcaggcacg catgatgatt aaccgcgcca cgctttatag cggttaatca
    15781 gaccattggt cgagctatcg tggctgctga tttctttgtc atctttcagc tctggcagaa
    15841 tacggttcgc cagttgttta cccaactcca cgccccactg gtcgaaggta aagatgttca
    15901 ggatcacgcc ctgagtaaag attttgtgct catacagcgc aatcaacgca cccaggctga
    15961 acggagtgat ttcgcgcagc aggatggagt tggtcgggcg gttaccttcg aacactttga
    16021 acggcaccac gtagtcaagc gttgccggat ctttaccctg atcgcgatat tcctgctcaa
    16081 ccacttcgcg ggatttacca aacgccagcg cttcggtctg ggcgaagaag ttagacagca
    16141 gtttctggtg gtgatcagag agcgggttat gggtgatagc cggggcgatg aaatcgcacg
    16201 gtaccatttt ggttccctgg tggatcagct ggtagaacgc gtgctgaccg ttagtgcctg
    16261 gttcacccca gataatcggg ccagtctggt aatccacaac gttaccgtta cggtcaacat
    16321 acttaccgtt ggactccata ttgccctgct ggaagtacgc cgcgaaacgg tgcatatact
    16381 ggtcatacgg cagaatcgct tcagtttccg caccaaagaa attgttgtac cagatgccaa
    16441 tcagcgccag caggacaggc aggtttttct cggcaggcgt ggtggagaaa tgcttgtcca
    16501 tcgcgtgtgc gccagaaagc agttcaacga agttatcaaa gccgatggag agaacaatcg
    16561 acaggccaat cgctgaccac aaagagtaac ggccgccgac ccagtcccag aactcgaaca
    16621 tgttggcagt atcaatacca aactcgccaa cggctttggc attggtggaa agcgccgcaa
    16681 agtgttttgc aacgtgtttt tcatcacctg ccgctttcag gaaccagtca cgcgcgctat
    16741 gggcgttggt catagtttcc tgagtggtga aggttttaga tgctaccagg aacagcgtgg
    16801 tttccgggtt tacttttttc agcacttcag cgatgtgagt cccatcgacg ttagaaacaa
    16861 agtgcatgtt caggtgattt ttgtacggac gcagtgcttc ggtcaccatg tacgggccga
    16921 ggtcagaacc accgatcccg atgttcacta cgtcagtgat tgctttgccg gtataaccct
    16981 tccactcacc ggagataatc gcttccgaga aggttttcat cttctccagc accgcgttga
    17041 cttccggcat tacgtctttg ccatcaacca gaatcggggt attgctacgg ttacgcagcg
    17101 ctacgtgcag cacggcgcgg ttttcagtgc ggttgatctt ctcgccagag aacatcgact
    17161 taatcgcgcc cgccagatcg cactctttcg ccagatcctg taatttcgcc agcgtctctt
    17221 cagtgatgcg gtttttggag taatccacca gcatctgatc gtcgaatgtt gcggagaact
    17281 tagaaaaacg atcgccgtct ttagcaaaaa gatcggcgat cgtaacgtct ttcatttcat
    17341 cgaagtgttt ctgtagtgcc tgccaggcag cggtctgcgt tggattgatg tttttcatta
    17401 gcaatactct tctgattttg agaattgtga ctttggaaga ttgtagcgcc agtcacagaa
    17461 aaatgtgatg gttttagtgc cgttagcgta atgttgagtg taaaccctta gcgcagtgaa
    17521 gcatttatta gctgaactac tgaccgccag gagtggatga aaaatccgca tgaccccgtc
    17581 gttgacaacc gccccgctca ccctttattt ataaatgtac tacctgcgct agcgcaggcc
    17641 agaagaggcg cgttgcccaa gtaacggtgt tggaggagcc agtcctgtga taacacctga
    17701 gggggtgcat cgccgaggtg attgaacggc tggccacgtt catcatcggc tacaggggct
    17761 gaatcccctg ggttgtcacc agaagcgttc gcagtcgggc gtttcgcaag tggtggagca
    17821 cttctgggtg aaaatagtag cgaagtatcg ctctgcgccc acccgtcttc cgctcttccc
    17881 ttgtgccaag gctgaaaatg gatcccctga cacgaggtag ttatgtctga aattgttgtc
    17941 tccaaatttg gcggtaccag cgtagctgat tttgacgcca tgaaccgcag cgctgatatt
    18001 gtgctttctg atgccaacgt gcgtttagta gtcctctcgg cttctgctgg tatcactaat
    18061 ctgctggtcg ctttagcgga agggctggaa cctggcgagc gattcgaaaa actcgacgct
    18121 attcgcaaca tccagtttgc cattctggaa cgtctgcgtt acccgaacgt tatccgtgaa
    18181 gagattgaac gtctgctgga gaacattact gttctggcag aagcggcggc gctggcaacg
    18241 tctccggcgc tgacagatga gctggtcagc cacggcgagc tgatgtcgac cctgctgttt
    18301 gtcgaaatcc tgcgcgaacg cgatgttcag gcacagtggt ttgatgtacg taaagtgatg
    18361 cgtaccaacg accgatttgg tcgtgcagag ccagatgtag ccgcgctggc ggaactggcc
    18421 gcgctgcagc tgctcccacg cctcaatgaa ggcttagtga tcacccaggg atttatcggt
    18481 agcgaaaata aaggtcgtac aacgacgctt ggccgtggag gcagcgatta tacggcagcc
    18541 ttgctggcgg aggctttaca cgcatctcgt gttgatatct ggaccgacgt cccgggcatc
    18601 tacaccaccg atccacgcgt agtttccgca gcaaaacgca ttgatgaaat cgcgtttgcc
    18661 gaagcggcag agatggcaac ttttggtgca aaagtactgc atccggcaac gttgctaccc
    18721 gcagtacgca gcgatatccc ggtctttgtc ggctccagca aagacccacg cgcaggtggt
    18781 acgctggtgt gcaataaaac tgaaaatccg ccgctgttcc gcgcgctggc gcttcgtcgc
    18841 aatcagactc tgctcacttt gcacagcctg aatatgctgc attctcgcgg tttcctcgcg
    18901 gaagttttcg gcatcctcgc gcggcataat atttcggtag acttaatcac cacgtcagaa
    18961 gtgagcgtgg cattaaccct tgataccacc ggttcaacct ccactggcga tacgttgctg
    19021 acgcaatctc tgctgatgga gctttccgca ctgtgccggg tggaagtaga agaaggtctg
    19081 gcgctggtcg cgttgattgg caatgacctg tcaaaagcct gcggcgttgg caaagaggta
    19141 ttcggcgtac tggaaccgtt caacattcgc atgatttgtt acggcgcatc cagccataac
    19201 ctgtgcttcc tggtgcccgg cgaagatgcc gagcaggtgg tgcaaaaact gcatagtaat
    19261 ttgtttgagt aaatactgta tggcctggaa gctatatttc gggccgtatt gattttcttg
    19321 tcactatgct tatcaataaa cgagcctgta ctctgttaac caacgtcttt atcgagaata
    19381 attgccttta atttttttat ctgcatctct aattaattat cgaaagagat aaatagttaa
    19441 gagaaggcaa aatgaatatt atcagttctg ctcgcaaacg aattcccgta cactgaactc
    19501 tctggctcac tgagggcacc atcatggcac tcccccgtat tacccaaaaa gagatgactg
    19561 aacgcgagca gcgtgaactt aaaacactgc tggatcgggc gcggattgcg catggtcgcg
    19621 tactgaccaa ctccgaaacc aacagcatta agaaagagta catcgataaa ttaatggtcg
    19681 agcgtgaggc tgaagcgaaa aaagcccgcc aattgaagaa aaagcaggct tataaaccgg
    19741 atcctgaagc gtcatttagc tggtcagcaa atacgtcgac gcgcggcagg cgctaatgaa
    19801 caatcaacgc ctgagatttt cactcgtcag tgtgaacaca attttcccct caccctaacc
    19861 cttttcccga aggggcgagg ggactgtccg agcacttttg ggccgttttc atcagtctga
    19921 ggcgttaatc agcgcccctt ctttttacgc cccggcgagg taaaacgctt accgttggac
    19981 gccgggcgac cgcctttttc cgccgttttt tccatcttaa cgactggacg tttgatgccc
    20041 gctgttttcg gtttggcctt cgctttgggc ttcgcctcgg aagaggaatt ttcaatgagc
    20101 ttaaagagat cgataagctc gtcgtcggtt aaatcgcgcc attctcccag cggaatgccg
    20161 cttaagctga cgttcataat gcgcgtacgt tccagctttt tcacttcata gccgaaatgc
    20221 tcgcacatgc gccggatctg acggttcaac ccctgcacca gggtaatgcg gaagacaaac
    20281 ggtgcttctt ttttgacttt gcactttttc gtcaccgtcc cgaggatcgg cactcccgcc
    20341 cccataccac gaataaattc atcggtaatc ggtttatcga ccgtcaccag atactctttc
    20401 tcatgatcgt tacccgcacg gaggatctta ttcaccagat cgccgtgatt ggtgaggaaa
    20461 atcagcccct gggagtcttt atccaggcgg ccaatcggga acacgcgttt gctgtggtta
    20521 acgaaatcga caatgttatc gcgctcgcca tcttcggtgg tgctgacaat accgacgggc
    20581 ttgttcaggg cgataagtac caaatcttcg gcttcccgag gctcaatcaa ctgaccgttt
    20641 actttcacaa tgtcgccggg tttcacctga tcaccaatgg tggctcgctt gccattaagg
    20701 aacacattgc cttgctcgat atagcgatcc gcttcgcggc gtgagcaaat tccgctttcg
    20761 ctgatgtatt tatttaaacg gactgatgag tcgggcagca taaattctcc tgtaaaacgc
    20821 ggaatatacc gtacctctta gcggggaaaa acagggttat gcggcaacgt cgaaattaaa
    20881 tgtgatctgc cacgcctttt atcacaaaag tgtctccgga agtacgatgc aaatttgcct
    20941 ggcaaaataa ggataatcaa catattacga atcgctgcac acctgtgctg atgactacta
    21001 cgcagagaaa caaatgtgca agagaaagcg ctatttctcc ccatgcaaag agtgaaaaat
    21061 cgctttgaaa ggccctcata atggggttat atcagatcaa gcggtgagaa agcgtatttg
    21121 cacaaatgtg cagaaccagg aggcggcaat ggaaaacagt gacgatatcc gtttgattgt
    21181 gaagattgcc caactctatt acgaacagga tatgacgcag gcgcaaatcg cgcgcgaact
    21241 gggtatttac cgcaccacca tcagccgctt gcttaaacga ggccgcgatc agggaattgt
    21301 caccatcgcc atcaactatg actacaacga aaatctctgg ctggagcagc aactgaagca
    21361 aaagtttggc ttgaaagacg ttgtggtggt gtcgggaaat gatgaggatg aagagactca
    21421 actggcgatg atggggttac acggcgcgca actgctggat cgcttgctgg agcctggcga
    21481 tattgtcggt ttttcctagg gtcgcgcggt gagcgcactg gttgaaaact tgccgcaagc
    21541 ggggcaatcg cggcagttaa tctgcgtgcc aattattggc ggaccgtccg gtaaactcga
    21601 aagccgctat cacgtaaaca cattaaccta cagcgtggca gcgaagctga aaggggaatc
    21661 gcatctcgcg gattttccgg ctctgctgga taacccctta attcgtaatg ggatcatgca
    21721 gtctcagcac tttaaaacca tctctgccta ctgggataat ctggatgtcg ccctggtggg
    21781 aattggctca ccggccattc gcgacggcgc taactggcat gcgttttatg gtggtgaaga
    21841 gagtgacgac ctgaaagcag gcgcggtatt ccattggcat tgccatgagt gaagaaaaat
    21901 acagcggaat tgttggtgca ctgcgtggaa aatatattaa ttgtctggta acgaatagca
    21961 gcacagctga actattactg aaataaaaat cttcccgatg tttaccgtaa taaaaattac
    22021 ggcgggtccc tattatctaa aatcaggagt ctgttatgca aacgtggtta aatttgcagg
    22081 ataaaatcat tatcgtcacc ggcggcgcat ccggtattgg tctggcgatt gtagaggaat
    22141 tattagcaca aggcgcaaac gtacagatgg tagatattca cggtggcgat ggtcaatatg
    22201 aagttcataa aggctatcag ttttggccga ccgatatttc cagcgccaaa gaggtaaatc
    22261 atacggtagc ggaaattatc cagcgttttg gtcgcatcga cggtctggtc aataacgccg
    22321 gggtcaattt cccgcgtctg ctggtcgatg agaaagcgcc tgccgggcag tatgaactca
    22381 acgaagctgc attcgaaaaa atggtcaata tcaaccaaaa aggcgttttt ctgatgtcgc
    22441 aggcggtggc gcgccagatg gtcaaacaac atgatggcgt gattgtgaat gtttcctcag
    22501 aaagtggact ggaaggctca gaaggccaaa gctgttacgc cgctaccaaa gccgcgctca
    22561 atagcttcac gcgctcctgg tcgaaagagc tgggtaaaca cggtatccgt gtggtcggta
    22621 tcgcgccggg gattctggaa aaaaccgggc tacgcacgcc ggaatatgaa gaggcgctgg
    22681 cgtggacgcg caatatcacc gtcgagcagc tgcgtgaagg ctataccaaa aacgccattc
    22741 ctattgggcg cgccggaaga ttagcagaag tcgctgattt tgtttgttat ctgctgtctg
    22801 aacgcgccag ctacatcacc ggagtaacca ctaacattgc gggcggcaaa acgcgcggat
    22861 aaggaggctt tatggtcaac gccatttttt gtgcccacgg caaattagct tgcgccatgc
    22921 tggaatcggt tcagatggtg tacggcgacg ccaatgtcga ggcggttgaa tttgtaccag
    22981 gcgagaacgc cggagacatc gtagcaaagc tggaaaagtt agtaagcatt cacaatcaag
    23041 atgagtggct gattgccgtg gatttacagt gcggcagccc gtggaatgcg gcagcgatgc
    23101 ttgcgatgcg caatcctcgt ctgcgggtaa tcagcggcct ttccctgccg ctggcgctcg
    23161 aactggttga taaccaggac agcatgaatg ttgatgaact gtgtgaacac ctgacgcaaa
    23221 tcgcaaagca aacctgcgtt gtctggaaac aactggcaac ggcagaggaa gatttctgat
    23281 gaacattacg ctcgcccgca ttgatgaccg tctgatccac ggccaggtca ctaccgtctg
    23341 gtcgaaagtg gctaacgccc agcgcattat tatctgcaat gacgaagttt ataacgatga
    23401 agtgcgccgg acattattgc gtcaggctgc gccaccggga atgaaggtta acgtggtcaa
    23461 tattgaaaaa gccgtcgcgg tttatcacaa cccgcaatat taggacgaaa ccgtttttta
    23521 tcttttcact cgcccacagg atgccttagc gatggttcgc cagggggtaa aaataggcac
    23581 cctgaatatt ggcggcatgg cctggcggcc cggtaaaaaa cagttaacca aagcggtttc
    23641 attagatgat gatgatatta acgcatttca tgagttaaat aatcttggcg tcataatgga
    23701 tttacgcgtc gtagcgtcag atccctcaat caatattatc gacaaaataa acgaacagtt
    23761 aatcgcaaat taaataatag cgcctgtgta catatgcttt aacaggcagg gatgacgaca
    23821 cttaaaaggt gacaaatcat ggaaataagt accctacaaa ttattgctat atttcttttt
    23881 tcctgtattg ccggaatggg cagcgtgctg gatgaatttc agactcatcg cccgttaatt
    23941 gcctgtacgg tgattggttt aattctcggt gatttaaaaa ccggaataat actcggtggt
    24001 acgctggaat tgatagctct cggctggatg aacgtcggcg cggcgcaatc tccggattct
    24061 gcactcgcca gcataatctc cgccattctg gttatcgttg gtcagcaaag catcgccacc
    24121 ggaatcgcca tcgcgttgcc tgtggctgcg gcaggccaag tgctgacggt gtttgcccgt
    24181 accatcaccg tagtgttcca gcacgcggcg gataaagcag cggaagaggc gcggtttcgc
    24241 actctcgata ttctgcatgt ctccgcgctt ggcgtgcagg cgctgcgcgt tgctattccg
    24301 gcactgattg tctcactgtt cgtcagcgcc gatatggtca gcaatatgtt aaacgccatt
    24361 cccgaatttg tgacccgtgg actgcaaatt gctggcggtt ttatcgtggt ggtcggttac
    24421 gccatggtgc tacgtatgat gggcgtgaaa tatttgatgc ctttcttttt cctcggtttc
    24481 ctcgcaggtg gctacctcga tctcagcctg ctggcgttcg gtggcgtcgg cgtgatcatg
    24541 gccctggtct acatccagct aaatccacag tggcgtaaag ctgaacctca actccaggcc
    24601 accgcctcca ccgcccttga ccaacttgac gattaacgga gccatcatgg aacagagaaa
    24661 aattacacgc agcgatctgg tgagcatgtt tctgcgctcc aacctgcaac aggcgtcctt
    24721 taactttgaa cgtattcacg gactgggctt ttgctacgac atgatccccg ccatcaagcg
    24781 actttaccca ttaaaagagg atcaggttgc ggcgctcagg cgacacctgg tgttcttcaa
    24841 taccacgcca gccgtatgtg gcccggtcat cggcgtcact gccgccatgg aagaggcgcg
    24901 ggccaacggc gcggaaattg atgacggtac cattaacggc atcaaagtcg gtctgatggg
    24961 accattggca ggagttggcg atccactggt ctggggaacg ctgcgcccga ttaccgctgc
    25021 gctcggcgca tctctggcac tttctggcaa catcctcggc ccgctgctgt ttttctttat
    25081 tttcaatgcg gtgcgtctgg cgatgaagtg gtatggccta cagctcggct ttcgcaaagg
    25141 ggtgaatatc gtcagcgata tgggcgggaa tgtgctgcaa aaactcaccg aaggcgcgtc
    25201 gattctcgga ctgtttgtga tgggcgtgct ggtcaccaaa tggacgtcaa tcaacgtacc
    25261 gttggtggtt tcacaaacgc atgccgccga tggctccacc gtcatcatga ccgtgcagaa
    25321 cattctcgac caactttgcc ctggtttgct ggcgctcggt ctgacgctac tgatggttcg
    25381 tctgctcaac aaaaaaatta acccggtatg gctgattttc gccctgtttg gcttagggat
    25441 tatcggcaat gcgctgggct tcctgtcctg attcttcgcc ccggcacgac tgccggggcc
    25501 atcgctcaac atgaggtggt ttatgaaaac aacagctctg cgtctttatg gtaaacgtga
    25561 tttacgcctg gaaacctttg accttcctga aatgcaggag gatgaaatcc tcgcgacggt
    25621 ggtcactgac agcctgtgcc tctcttcctg gaaagaggca acctgggtga aaaccataaa
    25681 aaagtacccg acgatgtggc gaccaaccca atcatcatcg gccacgagtt ttgcggcgat
    25741 attctggccg tgggtaaaaa gtggcagcac aaattccagc cgggtcagcg ttatgtgatt
    25801 caggccaacc tgaaactccc tgaccgcccg gactgccccg gctactcctt cccgtgggtg
    25861 ggcggcgaag ccacgcatgt ggttattccc aacgaggtca tggaacaaga ttgcctgctg
    25921 gcatacgacg gcgaaaccta ttttgaaggc tcgctggttg aaccgctttc ctgcgtgatt
    25981 ggcgcgttca acgccaacta tcatcttcag gaaggtagtt ataaccacac gatggggatt
    26041 cgcccgcaag ggcgcacgct gatcctcggc ggcaccggac caatgggatt gttggcgatt
    26101 gattatgcgc tgcatggacc agttaacccg tcgctgctcg tcattaccga taccgacaac
    26161 gacaaattga gttatgcgcg caagcactat ccatcagaac cgcaaacact gattcattat
    26221 ctcaatgcca ccgatgcagc atttgatacg ttaatggcgc tgagtggcgg tcacggcttc
    26281 gatgatattt tcgtctttgt gcctaatgaa ggactggtga ctctcgcctc ttccttgctg
    26341 gcgacagatg gttgcctgaa tttcttcgcc ggaccgcagg ataaacattt cagcgcgcca
    26401 attaatttct acgatgtgca ttatgcattt acccactacg tgggcacgtc aggcggcaat
    26461 accgacgaca tgcgcgcggc ggtcaaactg attgaagaga aaaaagtgca ggccgcaaaa
    26521 gtggtaacac atattcttgg gctgaatgcc gcgggcgaaa ccacgcttga attgcctgcc
    26581 gtcggcggcg gcaaaaagct ggtgtatacc gggaaatacc tgccgctgac gtcactcacg
    26641 cagattcagg atcaagcact ggcggcgatt ctggcgcgtc atcaggggat ctggtccggt
    26701 gaggcggagc aatatctgct cactcatgca gaggcaattt cccatgatta atcgcgctac
    26761 ttaactctgc atgtcgctgg ctggtcgccg cggcaacttc gcgacctgat gtgcggctga
    26821 ttgctgaagc ggcccaatgt gcacgagaag gataaaaaaa catcagcgcc ttaaggggcg
    26881 ctcaaattgg tggcaaagta cacattgttt atgccggatg cggaataaag ccttattcgg
    26941 cctacataat ctcgaaaatt caatatattg cagttatctg gtaggcctgg taagcgtagc
    27001 gcatcaggca atgctgtgtt tgtcatcagt ctcagcgccc ggtgcaatac gtgacatcgt
    27061 gagccatgtc agtaagtggt taaattcgca gtggtagcat gccttccgtg atttcttact
    27121 aaccggagaa gtaatggaac tgcttttatt gagtaactcg acgctgccgg gtaaagcctg
    27181 gctggaacat gcactgccgc taattgctga acagttgcag ggtcgccgct cagcggtgtt
    27241 tatccctttc gctggcgtaa cgcagacctg ggatgattac acagcgaaaa cggctgcggt
    27301 tctcgctccg ctgggtgttt ctgtcaccgg tattcatagc gttgtcgatc ccgttgccgc
    27361 gattgaaaat gctgagatcg tgattgtcgg gggcgggaat actttccagt tgctgaaaca
    27421 gtgccgcgag cgcgggctgc tggcaccaat tactgacgtg gttaaacgtg gtgcgctgta
    27481 tattggctgg agcgcaggcg ctaaccttgc ttgcccaacc attcgtacca ccaacgatat
    27541 gccgattgtc gatccgcaag gtttcgatgc gctaaatctg ttcccgctgc aaatcaaccc
    27601 gcacttcacc aacgcgctgc cggaaggcca taaaggtgaa acccgtgagc agcgtattcg
    27661 cgaactgctg gtcgtcgcgc cagaactgac gattattggt ctaccggaag gtaactggat
    27721 cacggtgagt aaaggtcacg ctacgctggg tggcccgaac accacttatg tgtttaaggc
    27781 cggtgaagaa gcggtgccgc tggaagcggg tcaccgtttt taagtgaatt gccggatgca
    27841 tgatccggca tcttgttcat agtgcctgat gcgacgcttg acgcgtctta tcaggcctac
    27901 aggattcaca atattaatac tcgtcccgtc cttcatcttc atccggctgt tccagcacac
    27961 tgtaagccac cgaacaaaac agcgagttca gacgctgcat atctcccagt aagcctaaat
    28021 gtagcgaact ggtttcaatg ctctgcacgt tttgctgatg caggcgatcg acgtgggcgt
    28081 gggaatagcg gcgattaaga atgcgaaagc gatgcttgct gcggcgcaaa cgacgagcgc
    28141 tggtgacatc gccagagaag aacaccgaca ttgccagctt taaattactg agcaattgct
    28201 catagagcgc atccagttcc ttcaacccat caagcgaaaa tgcccgccgt gctgccagcg
    28261 atttatcagc aatttcgctg cccatgcgct cgacgatatc ggaagcctgt tcaaggttga
    28321 gcgacatttc aataatctcc gcccagcgac gcgactcttc ttctgccagt tcctcttttg
    28381 gcattcgcgc cagatacagc ttaatggcgg tgtagagaac gttgatatca tctgccagct
    28441 tacgcagctc tttctcctgc cgtggctcgc cgtgcatcac tttattcagc ccttccatca
    28501 tctgttccat ggcgtcgcca atgcgcaggg tttcgcgcgc ggcgttcgcc agagcaagcg
    28561 tgggcgtatc cagcgcgctg acatccagat gtttaggccg tagctgggta tccagttccg
    28621 gttcatcgcg aataatcgtt ttgcaaaacc gtgccatcgg gtcaacaaat ggcagcatga
    28681 ccaggcaacg tacaaggttg tagaagacgt ggaaatagat caccagttcc gcttttggca
    28741 atgacaactt ccccattgtc tctgccagca aatggacaaa cggcaggata atcaggctac
    28801 ccaccagctt aaacagcaaa ctacccagcg cgacacggcg ggctgcggca ttggcggcac
    28861 tgttgttgag catcgccagc aggccggaac cgaggttagc accaatcacc agacagagcg
    28921 ccacggggaa ggagataatg cctgcggcgg tcagagtcgc ggtcagcagt acagcagcaa
    28981 ggctggagta gctgataatg gcgaacatcg cgccaatcag cgcatccagc agaatatcgc
    29041 cggtcagcga ggcaaagatc acctgaacgc cgtttgcctg ggtgatcggc gttacggcct
    29101 gcacaatcaa ctccagcgcc agcaaaatca gcccaagacc aataccgacg cggcccagtt
    29161 gcccggcgcg tgactgtttg cgtccgagga aaaaaatcac gccgataaaa ataagtaacg
    29221 gtgacagcca ggataagtcg aaggtgagaa tacgcgccat tagcgccgtc ccgacatctg
    29281 cacccagcac aatcaccaga gccggtgcga gggctaccag atcctgagcg acgaacgagg
    29341 tcaccagcat ggtggtggca ttactgctct gtaccagtgc ggtaacgccg atccccgcgc
    29401 aaaaggcgag cggctttttt tcgacgctcc ggctaaggac agtacgcaaa cgcgcgccga
    29461 agacgcgcat tacgccggtt cgaacaatat gagtccccca gaccagcagg gcgacggcag
    29521 aaagcaggtg aagcagcgtt aacacgagtt caggttctcc ttatcgttat atgttcctga
    29581 agcaattgaa tgaccaaagg accggaaaca cgctgactgg catctttact gtgccttttg
    29641 acattcgttt ttccggctaa gattattcca gcctgtttcc agtataagtg tttcatatta
    29701 ataaagagac agggcactca ttgagcgccc tgtttgttgt aaggaaaagt gacagatttg
    29761 tgaatcagtc cgcgtcatac cccagattcg gtgccagcca gcgctcaaca tcggagacgc
    29821 tcataccttt acggcgggca tagtcttcaa cctgatcgcg ctgaatttgc gccaccgcgt
    29881 agtacttgct gtccgggtgg ctgaaatacc aacccgaaac cgatgcacca ggccacatgg
    29941 cgaaagattc tgtgagtttc atgccagtat gtttttccac ttccagcagc tcccagatgg
    30001 tggctttttc cgtatgttcc gggcaggccg ggtagcccgg tgccggacgg atgccctggt
    30061 agttttcgcg gatcagttct tcgttgctga ggttctcgtt cggcgcatag ccccagtaga
    30121 ctttacgcac acgctcatgg agatactccg caaaggcttc ggctaaacgg tcggcaagcg
    30181 ctttcaccat gattttgttg taatcatcgt gctgcgcttc aaaggcatca gccagtgcgt
    30241 cctcttccag cccgccagtc acggcaaatg cgccgatgta atctgcttta ccagaaagct
    30301 tcggcgcaac gaagtcagcg agacagtagt tagcgaatcc cgttttttcg gtctgttgac
    30361 gcagatggtg gctgacgttg atcacatggg tgcgcgtttc gtcacggtag atttcaatgt
    30421 catcgcccac acggtttgcc gggaacaagc ccaccacgcc acgcggattc agcgttttct
    30481 cggcgcttaa tttatccagc atgtcgttgg cgtctttaaa cagccgctgc gcctcaacgc
    30541 ccaccacttc atcttccaga atgcgcggat acttcccggc cagcgaccag gtcataaaga
    30601 acggtgtcca gtcgatgtaa ttacgcagag tttcgatgct ggcttcaact tcctgcacgc
    30661 cgagacggtg cgccaccggc ggcgtgtaag cctgccagtc aaaagcgaag tcgttatcgc
    30721 gcgccgcttc cagcgtgacc ggtggtgtgc gcggtttctt gcgcccgtgc tgaatacgta
    30781 cggtttcata ctccttgcgg gtacgagcga caaaatcatc gcgctgagta tcggaaagca
    30841 gcgccgccac cacaccaacg gtgcgcgagg cattctgcac atacaccgtc gggccgctgt
    30901 agttctgctc aattttcacc gccgtgtgcg cttttgaggt cgtcgcgcca ccgatcagta
    30961 acggaatggt gaagccctga cgctccatct ctttcgccac gttaaccatc tcgtccagcg
    31021 acggcgtgat aagccccgaa agtccaatca gatcagcatt cacttcttta gcggtacgga
    31081 gaattttttc cgcaggcacc ataacgccga gatcgacaat ttcgtagttg ttacattgca
    31141 gcaccacacc aacgatattt ttaccgatgt cgtggacgtc gcccttcacg gtggcgatca
    31201 ccatcttgcc gttggttttg ccctgctctt tgctggcttc aataaacggt tcgaggtagg
    31261 ccactgcctg tttcatgacg cgcgccgatt tgaccacctg tggcaggaac attttcccct
    31321 cgccaaacag atcgccgacc acgttcatgc cgtccatcaa cgggccttca atcacttcaa
    31381 tcgggcgcgt agcctgctgg cgggcttctt cggtatcctg ctcgataaac tcagtaatgc
    31441 ctttgaccag cgagtattcc agacgtttat tcacttccca cgagcgccac tccgcctgct
    31501 gggcgttagc ggtgtcgtcg gttttgctgc cgcgatattt ctcggcaagc tccagtaaac
    31561 gttcagtgcc atcatcgcga cgattaagaa tcacatcttc caccgcgtcg cgcagttcag
    31621 cgggcaggtc gtcgtaaatc gccagttgcc cggcgttgac gatccccata tccatgccat
    31681 tgcgaatagc gtagtagagg aacaccgcgt gaatggcttc gcgcaccggt tcgttgccac
    31741 ggaatgagaa agaaacgtta gatacgccgc cggaaatcag cgcgtgcggc agttcgcgtt
    31801 tgatgtcttc gcaggcgccg ataaagtcct gcgcatagtt gttgtgctct tcaatgccgg
    31861 tggcgaccgc gaagatgttc gggtcgaaga tgatatcttc tggcgggaag ccaacctctt
    31921 cggtgaggat tttgtacgcc cgacggcaaa tctcgatttt ccgtgcgcgg gtatcggcct
    31981 gtccctgttc gtcaaaggcc attaccacca ccgctgcacc gtaacgacgc aacagtttcg
    32041 catgatggat aaaggcgtcg acgccctctt tcattgagat agagttaaca atgcctttgc
    32101 cctgaataca cttcagcccc ttttcgatga cctcccattt tgaggagtcg atcataatcg
    32161 gcacgcgagc gatatccggt tcaccggcaa tcagattgag aaaacgcacc atcgccgctt
    32221 cggcgtcgag catcccttca tccatattga tatcgataat ctgcgcgccg ttttccacct
    32281 gctggcgggc cacatccagt gcctcgctgt atttctcttc tttgatcagg cgcttgaact
    32341 tagcggaacc ggtgacgttg gtgcgttcac ccacgttcac aaacaggcta tcttcgccaa
    32401 tgttcagcgg ctccaggccg gacaaacggc aggctacggg gatttccggc agtttgcgcg
    32461 gcgctaatcc ttctactgca cgactcatcg ctgcaatatg ttgtggcgtg gtgccacagc
    32521 agccgccgac gatattgaga aagcccgctt ccgcccattc acgtatttgt tttgccatcg
    32581 tgtcggcgtc gagatcgtac tcaccaaagg cgttgggtag cccggcgttc gggtgcgcgg
    32641 tgacgtagca ttccgcaatc cgtgacagct cctgcacgta ctggcgcagt tcatcgggcc
    32701 ccagcgcaca gttcaggcca aaggtcagcg cttcggcgtg gcgcaatgag ttgtaaaatg
    32761 cttcggtggt ctgcccggag agcgtgcgcc cggaggcgtc ggtgatggtg ccagagatca
    32821 taatcggcag ctcaacgccc agcgtttcaa actccgtttt taccgcaaat accgccgctt
    32881 tggcgttaag ggtgtcgaaa acggtttcaa tcaggatcag atccgcacca ccttccacca
    32941 gcgctttggt ggactctcga taagccgcca ccagcccgtc aaaagtgata ttacgaaatg
    33001 ccggatcgtt gacgtccgga gaaatagacg ccgtgcggtt ggttgggccg agaacaccgg
    33061 caacgtagcg cggtttctcc ggcgtgcgcg cggtccactc gtcagcacag gcgcgcgcca
    33121 gtttcgccgc cgcaaagttg atttccgccg acagggattc catctggtaa tccgccatcg
    33181 caatggtcgt ggagttgaag gtgttggttt cgatgatatc cgcgcccgct tcaaagtagg
    33241 cgttgtggat agcggcgatc acttccggtt tactgagtac cagcaggtcg ttgttgcctt
    33301 tgaggtcgca tggccagtcg gcaaagcgtt caccacgaaa atcggcttcg ttcagtcgat
    33361 agctctggat catggtgccc ataccgccgt ccagcaccag aatacgttca tttaactgcg
    33421 cacgcagttg ttccactttg ctgctcacac ttgctcccga cacgctcaac ccagatttaa
    33481 taaaaattca acaaaccata ctggcataaa cgcatctgtg gtaaaagcga ccaccacgca
    33541 acatgagatt tgttcaacat taactcatcg gatcagttca gcaactattg cattaactaa
    33601 caataaaaat gaaaatgatt tccacgatac agaaaaagga gactgtcatg gtcgcaccca
    33661 ttcccgcgaa acgcggcaga aaacccgccg ttgccaccgc accagcgact ggacaggttc
    33721 agtctttaac gcgtggcctg aaattactgg agtggattgc cgaatccaat ggcagtgtgg
    33781 cactcacgga actggcgcaa caagccgggt tacccaattc cacgacccac cgcctgctaa
    33841 ccacgatgca acagcagggt ttcgtgcgtc aggttggcga actgggacac tgggcaatcg
    33901 gcgcacatgc ctttatggtc ggcagcagct ttctccagag ccgtaatttg ttagcgattg
    33961 ttcaccctat cctgcgcaat ctaatggaag agtctggcga aacggtcaat atggcggtgc
    34021 ttgatcaaag cgatcacgaa gcgattatta tcgatcaggt acagtgtact cacctgatgc
    34081 gtatgtccgc acctatcggc ggtaaattgc cgatgcacgc ttccggtgca ggtaaagcct
    34141 ttttagccca actgagcgaa gaacaggtga cgaagctgct gcaccgcaaa gggttacatg
    34201 cctataccca cgcaacgctg gtgtctcctg tgcatttaaa agaagatctc gcccaaacgc
    34261 gcaaacgggg ctattcattt gacgatgagg aacatgcact ggggctacgt tgccttgcag
    34321 cgtgtatttt cgatgagcac cgtgaaccgt ttgccgcaat ttctatttcc ggaccgattt
    34381 cacgtattac cgatgaccgc gtgaccgagt ttggcgcgat ggtgattaaa gcggcgaagg
    34441 aagtgacgct ggcgtacggt ggaatgcgct gactttttct ggcgggcaga ggcaatattc
    34501 tgcccatcat acctgagtgg caatagaata agggtgtctg ttaatcgcat tgacgccaaa
    34561 ataacttaat gtcatacact tcacacactt ttattaactc agcattattt ttaaacatca
    34621 aaccacttaa ttattaacaa aatcgcaaga tctcattcca ccgaaacttt tacggctgca
    34681 ttatgagatt gagcgattta tgatatttct ttagttgtaa tcatattaaa ttttttcact
    34741 gttctgatag ttaaaattta agacatcaat aaacaatgag atatttaaat gattactcgt
    34801 attcctcgta gttctttctc tgcaaatatt aataatacag cccagacaaa tgaacaccaa
    34861 accctgagtg aattgtttta taaagaactc gaggataaat tttctggcaa ggagctggcg
    34921 acgcctctat taaaaagctt ctcagagaac tgtagacaaa atggtcgtca tatctttagc
    34981 aacaaggatt ttgtcattaa attttccacg tccgtcttac aagctgataa gaaagaaatt
    35041 acgataatta ataaaacgaa aacacgacac tcactcaaac cattgctcca atatttgaag
    35101 aatacctaat ggaaatttta cctcaacgct cagacactct tgataaacaa gaattaaacc
    35161 taaaatcaga tagaaaagaa aaagaattcc caagaattaa acttaatggt caatgttatt
    35221 ttccggggcg accccaaaac cgtatagtat gccgacacat tgctgcacaa tatattaatg
    35281 atatttatca gaatgttgat tacaaacccc atcaagatga ttattcttca gctgaaaaat
    35341 ttctcactca cttcaacaaa aaatgcaaaa accagacttt ggcgttgatt tccagccgtc
    35401 ctgaggggcg ttgcgttgct gcctgcggtg atttcgggct agttatgaaa gcatattttg
    35461 acaagatgga atcaaatggc atcagtgtta tggcagccat attactggtg gataaccatg
    35521 ctttgacggt ccggctaaga ataaagaaca caactgaagg atgtacccat tacgtggttt
    35581 cggtttatga tcctaatgta actaacgaca aaataagaat catgagcgaa agcaaagagg
    35641 atattaaaca ctattctctg atggatttta tgaatgtaga ttatagcctc ctgaaatggt
    35701 caaatgatca tgttattaac caatctgttg caataattcc agcacttccg aaagaacagc
    35761 tattgatatt aaaaggat
//
LOCUS       DAESHI010000035        34981 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.035, whole genome shotgun sequence.
ACCESSION   DAESHI010000035 DAESHI010000000
VERSION     DAESHI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34981
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.035"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..815)
                     /locus_tag="KW011_001758"
     CDS             complement(<1..815)
                     /locus_tag="KW011_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBH7660108.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="KW011_001759"
                     /pseudo
     CDS             907..993
                     /locus_tag="KW011_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1397..1873)
                     /locus_tag="KW011_001760"
     CDS             complement(1397..1873)
                     /locus_tag="KW011_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HBH7660109.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="KW011_001761"
     CDS             complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="KW011_001761"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HBH7660110.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3308..4348
                     /gene="bioB"
                     /locus_tag="KW011_001762"
     CDS             3308..4348
                     /gene="bioB"
                     /locus_tag="KW011_001762"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBH7660111.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4345..5499
                     /gene="bioF"
                     /locus_tag="KW011_001763"
     CDS             4345..5499
                     /gene="bioF"
                     /locus_tag="KW011_001763"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HBH7660112.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5486..6241
                     /gene="bioC"
                     /locus_tag="KW011_001764"
     CDS             5486..6241
                     /gene="bioC"
                     /locus_tag="KW011_001764"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HBH7660113.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6234..6902
                     /gene="bioD"
                     /locus_tag="KW011_001765"
     CDS             6234..6902
                     /gene="bioD"
                     /locus_tag="KW011_001765"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBH7660114.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENAATGKYIN
                     LALL"
     gene            7481..9502
                     /gene="uvrB"
                     /locus_tag="KW011_001766"
     CDS             7481..9502
                     /gene="uvrB"
                     /locus_tag="KW011_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HBH7660115.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9694..10602)
                     /gene="yvcK"
                     /locus_tag="KW011_001767"
     CDS             complement(9694..10602)
                     /gene="yvcK"
                     /locus_tag="KW011_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HBH7660116.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10867..11011
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10999..11988
                     /gene="moaA"
                     /locus_tag="KW011_001768"
     CDS             10999..11988
                     /gene="moaA"
                     /locus_tag="KW011_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBH7660117.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12010..12522
                     /gene="moaB"
                     /locus_tag="KW011_001769"
     CDS             12010..12522
                     /gene="moaB"
                     /locus_tag="KW011_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HBH7660118.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12525..13010
                     /gene="moaC"
                     /locus_tag="KW011_001770"
     CDS             12525..13010
                     /gene="moaC"
                     /locus_tag="KW011_001770"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBH7660119.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13003..13248
                     /gene="moaD"
                     /locus_tag="KW011_001771"
     CDS             13003..13248
                     /gene="moaD"
                     /locus_tag="KW011_001771"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HBH7660120.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13250..13702
                     /gene="moaE"
                     /locus_tag="KW011_001772"
     CDS             13250..13702
                     /gene="moaE"
                     /locus_tag="KW011_001772"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HBH7660121.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            13839..14543
                     /locus_tag="KW011_001773"
     CDS             13839..14543
                     /locus_tag="KW011_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBH7660122.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14748..15461
                     /locus_tag="KW011_001774"
                     /pseudo
     CDS             14748..15461
                     /locus_tag="KW011_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
     gene            complement(15526..16237)
                     /locus_tag="KW011_001775"
                     /pseudo
     CDS             complement(15526..16237)
                     /locus_tag="KW011_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
     gene            complement(16237..17478)
                     /gene="clsB"
                     /locus_tag="KW011_001776"
     CDS             complement(16237..17478)
                     /gene="clsB"
                     /locus_tag="KW011_001776"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HBH7660123.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17475..18236)
                     /locus_tag="KW011_001777"
     CDS             complement(17475..18236)
                     /locus_tag="KW011_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBH7660124.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18369..18779
                     /locus_tag="KW011_001778"
     CDS             18369..18779
                     /locus_tag="KW011_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HBH7660125.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18741..19847)
                     /locus_tag="KW011_001779"
     CDS             complement(18741..19847)
                     /locus_tag="KW011_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH7660126.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19858..21000)
                     /locus_tag="KW011_001780"
     CDS             complement(19858..21000)
                     /locus_tag="KW011_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH7660127.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQIWQMQRAEDN
                     GQTFEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEA
                     LLSTEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLL
                     STLGMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPAR
                     YFVSTLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(20993..22729)
                     /locus_tag="KW011_001781"
     CDS             complement(20993..22729)
                     /locus_tag="KW011_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH7660128.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="KW011_001782"
     CDS             complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="KW011_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HBH7660129.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="KW011_001783"
     CDS             complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="KW011_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HBH7660130.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24620..25984
                     /gene="rhlE"
                     /locus_tag="KW011_001784"
     CDS             24620..25984
                     /gene="rhlE"
                     /locus_tag="KW011_001784"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HBH7660131.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="KW011_001785"
     CDS             complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="KW011_001785"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HBH7660132.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26818..28968
                     /gene="dinG"
                     /locus_tag="KW011_001786"
     CDS             26818..28968
                     /gene="dinG"
                     /locus_tag="KW011_001786"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HBH7660133.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28996..29958
                     /gene="ybiB"
                     /locus_tag="KW011_001787"
     CDS             28996..29958
                     /gene="ybiB"
                     /locus_tag="KW011_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HBH7660134.1"
                     /translation="MDYRKIIKEIGRGKKHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVAMGEAATISDGLARVNQAF"
     gene            30099..31184
                     /gene="hcxB"
                     /locus_tag="KW011_001788"
     CDS             30099..31184
                     /gene="hcxB"
                     /locus_tag="KW011_001788"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HBH7660135.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(31413..31673)
                     /locus_tag="KW011_001789"
     CDS             complement(31413..31673)
                     /locus_tag="KW011_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBH7660136.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="KW011_001790"
     CDS             complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="KW011_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HBH7660137.1"
                     /translation="MASGWANDDAVNEQINSTIENAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="KW011_001791"
     CDS             complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="KW011_001791"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HBH7660138.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32997..34981)
                     /locus_tag="KW011_001792"
                     /pseudo
     CDS             complement(32997..34981)
                     /locus_tag="KW011_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8271 a         8975 c         9490 g         8245 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccactgca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcgtt cagcttttta tactaacttg agcgaaacgg
     1321 gaaggtaaaa agacaaaaag ttgtttttaa tacctttaag tgataccaga tggcattgcg
     1381 ccatctggca gagtgattaa ctaaacatcg cagtaatcga ggcgcttgcc agagagtgga
     1441 aatgaacgtt aaacccgacc atcgcgccgc tggcaccttc atcgacatca atacgttcta
     1501 tatccagcgc gtgaacggta aaaatgtagc gatgagtttc gcctttcggc ggtgctgcgc
     1561 catcgtaccc ggttttacca aagtcggtac gcgtctgcaa aacgccgtct ggcattgcta
     1621 ccagaccaga gccaaaccct tgcggtaata cgcgggtatc agcgggtaag ttaacaacta
     1681 cccagtgcca ccagccggag ccggttggcg catccgggtc gtagcaggtg acaacaaaac
     1741 ttttcgttcc cgcaggaaca tcatcccacg ccagatgcgg tgaaatatta tcgccatcgt
     1801 aacccatgcc gttaaagaca tgacgatgcg gcaatttatc gccatcgcgc agatcgttac
     1861 tgatgagttt catgaaccct cctttcttgt ttgcagaaag tgtagccaga aaccctcacg
     1921 cggacttctc gttattggca aaaaaatgtt tcatcctgta ccgcgcggtt aaccgctgcg
     1981 gtcagacgct gcaactgttg cgggagaata atatagggcg gcatcaggta aatcagtttg
     2041 ccaaaaggcc ggatccagac accctgttcg acaaagaatt tttgcagcgc cgccatattc
     2101 accggatgag tggtttcgac cacgccaatg gcccccagta cgcgcacatc ggcaaccatt
     2161 tcggcatcac gggcgggggc aagttgctcg cgcagctgta cttcaatatc cgccacctgt
     2221 tgctgccagt cgccagattc gagaatcgcc aggctggcgt ttgctgccgc gcaggccagc
     2281 ggattgccca taaaagttgg cccatgcata aagcaaccgg cttcaccgtt actgatggtt
     2341 tctgcaacct cgcgcgtggt gagtgtggcg gaaagggtca ttgtgccgcc ggttaaggct
     2401 ttaccgaggc acaaaatgtc cggcgcgatt tctgcatgtt cacaggcaaa cagtttcccg
     2461 gtacgaccaa atccagtggc gatctcgtcg gcaatcagca agataccttc gcgatcgcat
     2521 attttgcgga ttcgttttaa ccattccgga tggtacatgc gcatcccgcc tgcgccctgg
     2581 acaatcggct caatgatcac cgccgcgatt tcatgacgat gcgccgccat caggcgggca
     2641 aagcccacca tatcgcgctc atcccattcg ccatccatac ggctttgtgg ggcgggagca
     2701 aacaagtttt ctggcagata gcctttccac aggctgtgca ttgagttgtc cggatcgcac
     2761 accgacatcg cgccaaaggt atcgccatga taaccattgc ggaaggtcag aaaacgctgg
     2821 cgcgcttcgc ctttggcttg ccagtactgc aacgccattt tcatcgccac ttccaccgct
     2881 acggaaccgg agtccgcgag aaaaacgcac tccagcggtt gcggcgtcat cgccaccagt
     2941 ttgcggcaca gctcaatggc tggcgcatgg gtgataccgc caaacatcac atgcgacatg
     3001 gcatcaattt gcgacttcat cgccgcatta agctgcgggt gattgtagcc gtggatcgcc
     3061 gcccaccagg acgacatacc gtcaaccagg cgtctgccgt cagacaaaat cagctcgcaa
     3121 ccttcggcgc tcaccaccgg ataaaccggc agcggggagg tcatggatgt gtatgggtgc
     3181 cagatatggc gttggtcaaa ggcaagatcg tccgttgtca taatcgactt gtaaaccaaa
     3241 ttgaaaagat ttaggtttac aagtctacac cgaattaaca acaaaaaaca cgttttggag
     3301 aagccccatg gctcaccgcc cacgctggac cttgtcgcaa gtcacagaat tatttgaaaa
     3361 accgttgctg gatctgctgt ttgaagcgca gcaggtccat cgccagcatt tcgatcctcg
     3421 tcaggtccag gtcagcacgt tgctgtcgat taagaccgga gcttgtccgg aagattgcaa
     3481 atactgcccg caaagctcac gctacaaaac cgggctggaa gccgagcggt tgatggaagt
     3541 tgaacaggtg ctggagtcgg cgcgcaaagc gaaagcggca ggatcgacgc gcttctgtat
     3601 gggcgcggcg tggaagaatc cccacgaacg cgatatgccg tacctggaac aaatggtgca
     3661 gggggtaaaa gcgatggggc tggaggcgtg tatgacgctg ggcacgttga gtgaatctca
     3721 ggcgcagcgc ctcgcgaacg ccgggctgga ttattacaac cacaacctgg acacctcgcc
     3781 ggagttttac ggcaatatca tcaccacccg cacttatcag gaacgcctcg atacgctgga
     3841 aaaagtgcgc gatgccggga tcaaagtctg ttctggcggc attgttggct taggcgaaac
     3901 ggtaaaagat cgcgccggat tattgctgca actggcaaac ctgccgacgc cgccggaaag
     3961 cgtaccaatc aacatgctgg tgaaggtgaa aggcacgcca ctggcggata acgatgatgt
     4021 cgatgccttt gattttattc gcactattgc ggtagcgcgg atcatgatgc cgacctctta
     4081 cgtgcgcctt tctgccggac gcgagcagat gaatgaacag actcaggcga tgtgctttat
     4141 ggctggcgca aactcgattt tctacggttg caaactgctg accacgccga atccggaaga
     4201 agataaagac ctgcaactgt tccgcaaact ggggctaaat ccgcagcaaa ctgccgtgct
     4261 ggcaggggat aacgaacaac agcaacgtct tgaacaggcg ctgatgaccc cggacaccga
     4321 cgaatattac aacgcggcag cattatgagc tggcaggaga aaatcaacgc ggcgctcgat
     4381 gcacggcgta ctgccgatgc cctgcgtcgc cgttatccgg tggcgcaagg agccggacgc
     4441 tggctggtgg cggatgatcg ccagtatctg aacttttcca gtaacgatta tttaggttta
     4501 agccatcatc cgcaaattat ccgcgcctgg cagcagggcg cagaaaagtt tggtgtaggt
     4561 agcggcggtt ccgggcatgt cagcggttat agcgtggtgc atcaggcgct ggaagaagaa
     4621 ctggccgagt ggcttggcta ttcgcgggcg ctgctgttta tctctggttt tgccgcgaat
     4681 caggcagtca ttgccgcgat gatggcgaaa gaggaccgta ttgttgccga ccggcttagc
     4741 catgcctcct tgctggaggc tgccagttta agcccgtcgc agcttcgccg ttttactcat
     4801 aacgatgtcg ctcatctggc gcgactgctt gcttccccct gtccggggca gcaactggtg
     4861 gtgacagaag gcgtgttcag catggacggc gatagcgcgc cactggcgga aatccagcag
     4921 gtaacgcaac agcacaatgg ctggttgatg gtcgatgatg cccacggcac gggcgttatc
     4981 ggggagcagg ggcgcggcag ctgctggctg caaaaggtaa aaccagaatt gctggtggtg
     5041 acttttggca aaggatttgg cgtcagtggg gctgcggtgc tttgctccag tacggtggcg
     5101 gattatctgc tgcaattcgc ccgccacctt atctacagca ccagtatgcc gcccgctcag
     5161 gcgcaggcat tacgtgcgtc gctggcggtc attcgccgtg atgaggggga tgcacggcgc
     5221 gaaaaactgg tgtcactcat tgcgcgtttt cgtgccggag tacaggattt gccgtttacg
     5281 cttgctgatt catgcagcgc catccagcca ttgatcgtcg gtgataacag ccgtgcgtta
     5341 caactggcag aaaaactgcg ccagcaaggc tgctgggtca cggcgattcg cccgccaacc
     5401 gtacccgctg gtactgcgcg actgcgctta acgctaaccg cagcgcatga aatgcaggat
     5461 atcgaccgtc tgctggaggt gctgcatggc aacggttaat aaacaagcca ttgcagcggc
     5521 atttggtcgg gcagccgcgc actatgagca acacgcagag ttacagcgcc ggagtgctga
     5581 cgtcttactg gcaatgctcc cacagcgtaa atacacccac gtactggacg cggggtgtgg
     5641 gccaggctgg atgagccgcc actggcgaga gcgtcacgca caggtgacgg cgttagatct
     5701 ctcaccgcca atgcttgtgc aagcccgcca gaaagatgct gcagaccatt atctggcggg
     5761 agatatcgaa tccctgccgt tagcgactgc gacgttcgat cttgcatgga gcaatctcgc
     5821 ggtgcagtgg tgcggtaatt tatccacggc actccgcgag ctgtatcggg tggtgcgccc
     5881 cggaggcgtg gtggcgttta ccacgctggt gcagggatcg ttacccgaac tgcatcaggc
     5941 gtggcaggcg gtggacgagc gtccgcatgc taatcgcttt ttaccgccag atgaaatcga
     6001 acagtcgctg aacggcgtgc attatcaaca tcatattcag cccatcacac tatggtttga
     6061 tgatgcgctc agtgccatgc gttcgctgaa aggcatcggt gctacgcatc ttcatgaagg
     6121 gcgcgacccg cgaatattaa cgcgttcgca attgcagcga ttgcaactgg cctggccgca
     6181 acaacagggg cgatatcctc tgacgtatca tctttttttg ggagtgattg ctcgtgagta
     6241 aacgttattt tgtcaccgga acggataccg aagtggggaa aaccgtcgcc agttgtgcac
     6301 ttttacaagc cgcgaaggca gtaggttatc ggacggcagg ttataaaccg gtcgcctctg
     6361 gcagcgaaaa gaccccggaa ggcttacgta atagtgacgc gctggcgtta cagcacaaca
     6421 gcagcctgca gctggattac gcaacagtaa atccttacac cttcgcagaa cccacttcgc
     6481 cgcacatcat cagcgcgcaa gagggcaggc cgatagaatc attggtaatg agcgccggat
     6541 tacgcgcgct tgaacaacag gctgactggg tgttagtgga aggtgctggc ggctggttta
     6601 cgccgctttc tgacactttc acttttgcag attgggtaac acaggaacaa ctgccggtga
     6661 tactggtagt tggtgtgaaa ctcggctgta ttaatcacgc gatgttgact gcgcaggcaa
     6721 tacaacacgc cggactgact ctggcgggtt gggtggcgaa cgatgttacg cctccgggaa
     6781 aacgtcacgc tgaatatatg accacgctca cccgcatgat tcccgcgccg ctgctgggag
     6841 agatcccctg gcttgcagaa aatgcggcaa ccggaaagta cataaacctt gccttgttgt
     6901 agccattctg tatttggtta aattgcgagc gagatcgcgt cttcgattga ctgcaattta
     6961 accaattaaa ttctaaaata atcacgaaaa aaattttact tccgcctcat gcggcgaatg
     7021 tgggaattgc ccaggcggcg ggggataggg gctggagaca gttatccact attcctgtgg
     7081 ataaccatgt gtattagagt tagaaaacac gaggcaagcg agagaatacg cggcttgcac
     7141 gcgaattggc gttaaagacg gctcaaagaa atatctttta ttttttaact ggttagataa
     7201 atgcaatggc agtcactgaa caggcatctc ttgccataaa actgtcatca ctcatcttga
     7261 caaatgttaa aaaagccgtt gctttgggga taacccggta aggccggagt tttatctcgc
     7321 cacagagtaa attttgctca tgattgacag cggagtttac gctgtatcag aaatattatg
     7381 gtgatgaact gtttttttat ccagtataat ttgttggcat aattaagtac gacgagtaaa
     7441 attacatacc tgcccgccca actccttcag gtagcgactc atgagtaaac cgttcaaact
     7501 gaattccgct tttaaacctt ctggcgatca gccagaggcg attcgacgtc tcgaagaggg
     7561 gctggaagat ggcctggcgc accagacgtt acttggcgtg actggctcag ggaaaacctt
     7621 caccattgcc aatgtcattg ctgaccttca gcgcccaacc atggtacttg cgcccaacaa
     7681 aacgctggcg gcccagctgt atggcgaaat gaaagagttc ttcccggaaa acgcggtgga
     7741 gtatttcgtc tcctactacg actactatca gccggaagcc tatgtaccga gttccgacac
     7801 tttcattgag aaagatgcct cggttaacga acatattgag cagatgcgtt tatccgccac
     7861 caaagcgatg ctggagcggc gtgatgtggt tgtagtggcc tctgtttccg cgatttatgg
     7921 tctgggcgat cctgatttat atctcaagat gatgctccat ctcacggtcg gtatgattat
     7981 cgatcagcgc gcgattctgc gccgactggc ggagctgcaa tacgctcgta atgatcaagc
     8041 attccagcgt ggtactttcc gcgttcgtgg cgaggtgata gatatcttcc cggcagaatc
     8101 ggatgacatt gcacttcgcg tggaactgtt tgacgaggaa gtggaacgat tgtcgttatt
     8161 tgacccgctg accgggcaga ttgtttccac tattccacgt tttaccatct acccgaaaac
     8221 gcactacgtc acaccgcgcg agcgcatcgt acaggcgatg gaggagatca aagaagaact
     8281 ggccgccaga cgcaaagtgc tgttggaaaa caacaaactg ctggaagagc agcggctgac
     8341 ccagcgtacc cagtttgatc tggagatgat gaacgagctg ggctactgtt cggggattga
     8401 aaactactcg cgcttcctct ccggtcgtgg accgggtgag ccaccgccga cgctgtttga
     8461 ttacctgcct gccgatgggc tgctggtggt cgatgaatct cacgtcacca ttccacaaat
     8521 tggcggcatg tatcgcggtg accgggcgcg taaagagaca ctggtggagt acggcttccg
     8581 cctgccatca gcgctggata accgtccgct taagtttgaa gagttcgaag cattagcgcc
     8641 gcaaaccatc tatgtttcgg cgacgccggg taattacgag ctggaaaaat ccggcggcga
     8701 tgtggtggat caggtggtgc gtccaaccgg attgcttgac ccgattatcg aagtgcggcc
     8761 ggtggcgaca caggttgatg atcttctttc ggagattcgt cagcgagcgg caattaacga
     8821 acgcgtactg gtcaccacac tgaccaagcg gatggcggaa gatcttaccg aatatctcga
     8881 agaacatggc gagcgcgtgc gttatcttca ttcagatatc gacaccgtcg aacgtatgga
     8941 gattatccgc gacttgcgtc tgggtgagtt cgacgtgctg gtagggatca acttactgcg
     9001 cgaaggtctg gatatgccgg aagtgtcgct ggtggcgatc ctcgacgctg acaaagaagg
     9061 cttcctgcgt tccgaacgtt cgttgatcca gactattggt cgtgcggcac gtaacgttaa
     9121 cggtaaagcg attctctacg gcgataagat caccccatca atggcgaaag cgattggcga
     9181 aaccgaacgt cgccgtgaga aacagcagaa gtacaacgag gaacacggaa ttacgccgca
     9241 aggcttgaac aagaaagtgg tcgatatcct ggcgctgggg cagaacattg ccaaaaccaa
     9301 agcgaagggc agaggaaaat cgcgcccgat tgttgagccg gataatgtgc cgatggatat
     9361 gtcgcctaaa gcgttgcaac agaaaatcca tgagctggaa gggttaatga tgcaacacgc
     9421 gcagaatctg gagttcgaag aagcggcaca aattcgtgac cagttgcatc agctgcgtga
     9481 gctgtttatc gcggcttcgt gacaggataa cgaagaagac agatgacaaa agcaaaattg
     9541 cctgatgtgc tacgcttatc aggcctacat gatctctgca atacattgaa ttagcgcggt
     9601 ttgtaggccg gataaggcgt tcacgccgca tccggcataa acaacgcgca ctttgtcttc
     9661 aataggaagc cggaattttt cttcggattt ccgttaacct aaagcctgta acgccttttc
     9721 cagcgcgtta tgtaacaact ggcggtcatg acgatacgga atatcgctgg cctccagtac
     9781 ctcctgaatc acaatccgct ctttcaccgc cgagacatcc acttttggcc cgacgatgac
     9841 cgcatcaatg acctttttac caacatactg ctccataatt gccagcttgc tttccagctt
     9901 caaattagcc gcaggtaaac tcaactcacg ccccagattg ccgatataga ccatcggcgc
     9961 tggcgtgcgg cgtaatgcct gggcgatttc cttcagcagc agaattggca tcaggctggt
    10021 ataaaaactg ccaggcccaa taatgatgag atccgcttca ttgatagcgt gaaccgcctc
    10081 acgcgttgcg ggtacattcg gcgttaataa taactcttga atcggcgtag ttaactggtc
    10141 gatattgacc tcgccgtaaa cttcatgccc ctgatcgtca atcgccatca gatcgacggg
    10201 atgctctgac attggaatca aatgcgtatc cactttcagc agattacgaa ttaaattgat
    10261 ggcttccaga ggccgcacgc taaggtgatc cagcgccttt aacatcaagt ttccgagatt
    10321 atgaccggaa agttcgccat tgccgccaaa acggtattca aacatcgcgg aggcgacgct
    10381 cggttccgtt atcagttggt tgaggcagtt gcgcatatcg ccccaggcaa tgccgccttc
    10441 tgaacggcga atacgccccg tcgagccacc attatccgtg gtggtgacga tacccgttaa
    10501 acgagaaccc aaagacgaaa gtgatgagag aacgcgtccc agtccatgcc ctccgccgag
    10561 agcaacgaca cgatcaagat cagccagcgt acgattgcgc atatagtttc ctggagtcag
    10621 attatccgcg ctacagtagc gcaaattgcg gggaaacagc aattaacctg ccaatattga
    10681 ggatacaaac tattttcttc tacctctaaa ggacgatgca cgctatgcct ccctgatgat
    10741 gtatatcaaa gtaaaactgc cattttccct tattctgtag cgaaatagca cgatcatgac
    10801 gctatataca tgattacata gcgaaagtgt ggatggtaaa aatctcattt acacgctagt
    10861 atcggcttaa ccactaaaca ctctagcctc tgcacctggg tcaactgata cggtgctttg
    10921 gccgtgacaa tgctcgtaaa gattgccacc agggcgaagg aagaaatgac ttcgcctccc
    10981 gtatctggaa aggtgtacat ggcttcacaa ctgactgatg catttgcgcg taagttttac
    11041 tacttgcgcc tgtcgattac cgatgtgtgt aactttcgtt gcacctactg cctgccggat
    11101 ggctacaaac cgagcggcgt caccaataaa ggctttctta ccgtcgatga aattcgccgg
    11161 gttacgcgcg ccttcgccag tctgggcacc gaaaaagtgc gcctgacagg aggagagccg
    11221 tctttacgcc gcgactttac cgatatcatc gccgctgtgc gggaaaacga cgctatccgc
    11281 cagattgcgg tcaccaccaa tggttaccgt ctggaacgcg atgtggcgaa ctggcgcgat
    11341 gcgggactta ctggcattaa cgtcagtgtc gacagtctgg acgcccgcca gtttcacgct
    11401 attaccgggc aggataaatt caaccaggtc atggcaggga tcgatgctgc atttgaggtc
    11461 ggttttgaga aggtcaaagt caataccgtg ctgatgcgtg atgttaatca tcaccagctc
    11521 gacacctttc tgaactggat ccagcatcgc cctatccagc tgcgtttcat cgaactgatg
    11581 gaaacgggcg agggcagtga gctcttccgt aagcatcaca tctctggtca ggttctgcgt
    11641 gacgagctac tgcgtcgcgg ctggatccac caattacgtc aacgcagcga cggtcccgcg
    11701 caagtctttt gccatccaga ttacgccgga gagattggcc ttatcatgcc gtatgaaaaa
    11761 gacttctgcg ccacttgcaa ccgcctgcgc gtttcctcca ttggtaaact ccatctctgc
    11821 ctgtttggtg aaggcggcgt taacctgcgc gatctgctgg aagacgatac ccagcaacag
    11881 gcgctggaag cgcgtatttc agcggcgctg cgggagaaga aacagaccca tttcctgcat
    11941 caaaacaaca ccggtattac gcaaaactta tcgtacattg gcggctaaaa cgtcaaaagg
    12001 agagatcaga tgagtcaggt aagcactgaa tttatcccga cccgtattgc tattcttacg
    12061 gtttctaatc gtcgcggtga agaagacgat acctccggtc actatttgcg cgattcggcg
    12121 caagaagcgg gccatcacgt tgtcgataaa gccattgtga aagaaaaccg ctacgctatt
    12181 cgcgctcagg tatctgcgtg gatcgccagc gacgatgtac aagtggtgtt gattacgggg
    12241 gggactggcc tgacggaagg tgatcaggct cccgaagcat tgctgccgtt gttcgaccgt
    12301 gaagttgaag gttttggtga agtgttccgg atgttgtcgt ttgaagagat tggcacttcc
    12361 acgttgcaat ctcgtgcggt agcgggcgtc gccaacaaaa cgctgatttt cgccatgccg
    12421 ggttcgacca aagcgtgccg taccgcatgg gaaaatatca tcgcgccgca gctggatgcc
    12481 cgtacgcgtc cgtgtaattt ccatccacat ttgaagaaat aagtatgtcg caactgaccc
    12541 atatcaacgc cgctggcgaa gcgcacatgg tggatgtctc cgccaaagcg gaaaccgtgc
    12601 gtgaagcgcg ggcggaagcc tttgtcacca tgcgcagcga gacgctggcg atgattattg
    12661 atggtcgcca ccacaaaggc gacgtatttg ccactgcgcg tattgtcggt attcaggcgg
    12721 caaaacgcac ctgggatctg atcccgctgt gtcacccgct gatgctcagc aaagttgaag
    12781 tcaatttaca ggccgagccg gagcacaatc gggtgcgtat agaaacctta tgccgcctga
    12841 ccgggaaaac cggtgtcgaa atggaagcgt taaccgcggc ctccgtggcg gcgctgacca
    12901 tttatgacat gtgcaaagcg gtgcaaaaag atatggtgat tggtccggta cgtttgctgg
    12961 cgaagagcgg cggcaagtcg ggtgacttta aggtggaagc ggatgattaa agttcttttt
    13021 ttcgcccagg tgcgcgagtt ggtgggaaca gatgcaaccg aagtggctgc ggattaccca
    13081 accgttgaag cgttacgcca gcacctggct gcgcagagcg atcgctgggc gctggcgctg
    13141 gaagatggca aattgctggc tgccgtcaac cagacgctgg tgagttttga ccatccgctg
    13201 actgacggcg acgaagtagc tttcttcccg ccggtaaccg gaggttaaga tggcagaaac
    13261 caaaattgtt gtcggcccgc agccgttcag cgtaggagaa gagtacccgt ggctggctga
    13321 gcgtgacgaa gacggtgcag tggtcacgtt taccggtaag gtgcgcaacc ataacctggg
    13381 tgacagcgtc aaagcattaa ccctcgaaca ctatccgggg atgactgaaa aagcactggc
    13441 agaaatcgtt gatgaagcgc gtaaccgctg gccgctgggg cgcgtcacgg tgattcaccg
    13501 catcggggaa ttatggccgg gcgatgaaat cgtttttgtc ggtgtcacca gtgcgcatcg
    13561 cagcagtgcg tttgaagccg ggcagtttat tatggattat ctcaaaaccc gcgcaccgtt
    13621 ctggaagcgc gaagccacgc cggaaggcga ccgctgggtt gaagcccggg agagcgatca
    13681 gcaggcggca aaacgctggt agttttttgt tagccggata aggcaccgct gcgtccggca
    13741 atttcaccgc tcagtacatg cgtttacgct gcgtcgatgc acagcctcat cattttgcag
    13801 tatccttaag atattcctta tatcttcagg agatcgtcat ggacagattc ccacgttctg
    13861 attcaatcgt acaaccccgg gctggcttgc aaacttatat ggcgcaagtc tatggctgga
    13921 tgaccgttgg cttgttgctg accgcatttg ttgcctggta tgcggctaat tccgcggccg
    13981 tgatggagct gttgttcact aaccgtgtct ttttaatcgg tctgatcatc gcgcaattag
    14041 cattggttat tgtgttatca gcgatgattc aaaagctgag cgcaggtgta acgacgatgc
    14101 tctttatgct ttattcggcg ctgacgggtc ttacgctttc cagtatattc attgtctata
    14161 ccgctgcttc tatcgccagt actttcgtcg ttactgccgg gatgttcggc gcaatgagcc
    14221 tgtacggtta caccacgaag cgcgatttaa gtggctttgg caatatgctg tttatggcgt
    14281 taatcggcat tgtgctggca tcgctggtca acttctggct gaaaagcgaa gcattgatgt
    14341 gggcagttac ctacatcggc gtgattgtct ttgtcggatt gacggcgtat gacactcaga
    14401 aattgaaaaa tatgggtgag cagattgata cccgcgacac gtcgaacctg cgcaaatatt
    14461 ccattcttgg cgcgttaacc ttgtatctgg acttcatcaa cctgttcctg atgttgttac
    14521 ggatcttcgg caaccgccgt taattctttt tcaccatcac tatcccggcc atgtgtcggg
    14581 atttttttct tactaaaaac gacacttaac aagtaccagg tataaaatat gccttatgag
    14641 cgagcggaat cgttttccgt atccataaat acaaaagtat tttgtatgcg tggttttaaa
    14701 tttattgatg gtgaatatta atattttttc taaggacaac taaaagaatg gaatcatact
    14761 cgcaaaacag caataaatta gattttcagc acgaggccag gatataaaac ggtatatggc
    14821 tcattaccgc tttaggtttg gtggcaaccg caggactagc ctggggagct aagtatctcg
    14881 aaattacggc aaccaaatat gattcaccac caatgtatgt cgctataggg ttattattac
    14941 tttgtatgta tggcttaagt aaggatatca acaagataaa tgccgccatc gcgggcgtaa
    15001 tatatctgtt tttactctct ttggtggcga ttgtcgttgc aagtttagtt cctgtatatg
    15061 ccattatcat cgtgttcagc actgcgggcg cgatgttttt aatcagtatg ctggccggtt
    15121 tattatttaa tgttgagcct ggttctcacc gttttatcat tatgatgacg ttgacagggt
    15181 tggccctggt aatcatcgtg aatgcggcat taatgagtga acggcccatt tgggtaataa
    15241 gttgcttaat gattgtgtta tggccaggca ttatctcgca tggacgaaat aagctccttg
    15301 aactggcagg aaaatgccat agtgaagagt tgtggagtcc ggttcgttgc gcttttacag
    15361 gtgcattaac actctattac tattttatcg gcttctttgg gatacttgcc gcgatagcta
    15421 taacgcttgt ctggcaaagg catacgtgtt tttttcatta gccgtaaatg cacatactaa
    15481 agaacttaac taaacttcac atcgccgctt catttttcgc ccgtagccca gtacaaagaa
    15541 atagttcacg ctttgaccaa gtaacagcca gataatcgcc cccattacca tccagttaac
    15601 gctggaaatc agcatctgag cgagggcgaa tttccatgaa ggcagcacca gcttttgccc
    15661 tttgatggtc atatggcggt gcttcgcgaa agcgcaaaac cacaaataaa ccgcgataat
    15721 catcagtaag ccaatgccga gaatacgcag cgtagtttga tcgacatacc agtgatccgg
    15781 caactccacc acgcctgcgg taaagataat ccctgccagt aaaatatagc ccagccagtt
    15841 ggtggtaata ctgagcgaga aaatccgcgt aatagtgcta ccgggcaacc ccagacgaga
    15901 gtacaaacga tagcgcatac caatgccgcc gacccaggta ctgagcgtca ggttgaaggc
    15961 gtagcagata aacgacacca gcatcacctg gcgcttcgcc agtttgtgac cgcagtaaaa
    16021 gcgggcgagc aggtcatagc agccgtaaat cagatagctg acgaccacca gcgaccgcac
    16081 taagcagcgc aacgcgattg tagtcgcgga tgaccttcca gacctcttcc cagtccactt
    16141 ttttggcgta gaccaccagc aacacaatca ccgcgataaa aaacagccag gtgaggatct
    16201 tctttgctaa gcgccagcgc gggtgtgatt tactcatcag ggttttaccc ccgtgttttc
    16261 agtttctacc cgatcctgcg tttccattgt cggttgtgcg ggcggatcaa cctgcgccag
    16321 acgtggcgtg tgtgccggaa gccagccaac cagcgccggg aagtggcgta aaaagtggaa
    16381 cgccagcacg cttttggtca ggttccacca ggtgcgtttg ggcagcatgg tttcatccac
    16441 ctgctgacaa tctgcggcga taatgccgtt cagattatcg cgcagcgtct ggttaaaatt
    16501 acgatcgtgg atgatgacat ttgcttcgag attcagtgac aaactgagcg gatcgagatt
    16561 actggaccct actgtcgccc agtgatcgtc catcaatgct actttgccgt ggagcgggcg
    16621 gcggcggtac tcaaacacct gaacgccgcc tttaaccaga tagttataca gcaagcgcgc
    16681 gccgactctg acaatcggca tatccggttc gccctgaatg atcagtttga tccgcacccc
    16741 gcggcgtgcc gctttacgca aggcgtgtaa aaatcgatag ccggggaaga agtaggcgtt
    16801 ggcgataatg acttcccgcc gcgcctgagt gagcattttc agatagtggc gttctatatc
    16861 atcgcggtgt tcttcgttat cgcgccagac cagcaatacc tgcgcgtctc ccggctggcg
    16921 gttctcttcc gctttgtgat gacgtcgcca ccagcgtcgt gccgcgctct gtccaggcag
    16981 gttttccagc tcaaactgga gaatatcttc gacaatcggc ccttcaaggc gtaccgcgta
    17041 atcctgttta gcctctggac cgtagctgga catatgctcg gcggagtaat tcagcccgcc
    17101 aataaaggcg atacgcgcgt cgatcaccac aattttgcga tgcatccggc gaaacacatt
    17161 ggtgcgcata ccaaaaaggc gagggcgggg atcgtagtag cggaacacta cgccagctgc
    17221 cgtcagttca ttgacaaact catcgctgag atccggcgaa ccgtagccat ccagcaagac
    17281 ttccgcttta accccgcgtt gcgctgctgc cagtagtgcc gcatgcagct gtttgccgac
    17341 gttatcctca aaccagataa acgtttcaag aatgatgcgt tcttgtgcct cgccaatcgc
    17401 cttaaacacc gcgggataat attgctcgcc gttttccagc aactggatct tattgccttc
    17461 gcgccagcta catttcataa atgaatctcc gcacttaaag gggcatgatc agaaaggtgt
    17521 cgccatgtcc gcagcggcaa cgcggttggc gcgctggcgc tggcattttt gacgtagatc
    17581 ctgtccagtc gtagtagagg aaattgcacc ggaaacgtgc gcgccgggcg tccgtgggcg
    17641 cgggtaaaaa tctcatccag tccggcctgc acttttaacg gatgattagc tttttgccgc
    17701 cagtcattga aatcacccgc caccaatacc ggttcgccgt ccggtagctc attcacccat
    17761 tcggcgagca tcgcaagctg cgcctgacgg tgcgcctcac gcaggcccag atgtacgcac
    17821 atcacatgaa tcgcttttcc ggtcatcggc ggcacaatgc ggcagtagag cacgccgcgc
    17881 ttttccgcac catcgaccga aacatcgcga ttctcataat gttcaatggg ataacgcgac
    17941 agtacggcgt tgccgtgatg cccttccggg tatacggcat tgcgaccgta ggcaaaatcg
    18001 ctccacatag tgtcggcgag aaactcgtag tgcgaggtat cgggccagtt ttccacatgc
    18061 agcggatgaa cttcgtgcgc gcccatcact tcctgcaggc aaacaatatc ggcgctgacg
    18121 gtacgcacgg catcgcgaag ttccggcaaa atgaagcgtc ggttaaacgc ggtaaagcct
    18181 ttgtgaatgt tgatggtgag caccttgaac gaaaattgtt gtgtttgatc gggcataatt
    18241 ttcctgtctt tgcctcttat ctcattgaaa tagtgtagtc ggcgtcacaa aaaggtgcgg
    18301 tcttacggaa ttttccgtaa agttcggtac tctgagtaag tagagataaa ttcttcagga
    18361 gagaagccat gaagtggcaa caacgtgttc gtgtcgcaac gggtctaagt tgctggcaga
    18421 ttatgttgca tttactggta gtggcgctgc tggtggtggg ctggatgagt aagactctgg
    18481 ttcacgtcgg cgtgggatta tgcgcactgt attgtgtcac ggtagtgatg atgctggtgt
    18541 ttcagcgcca ccccgagcaa cgctggcgtg aggtggcaga cgtgctggaa gagctgacca
    18601 cgacctggta ttttggcgca gcgctgattg tgctgtggct gttgtcccgc gtactggaaa
    18661 acaacttttt gctggcaatt gcagggctgg cgatccttgc tggcccggcg gtagtgtctt
    18721 tgctggcgaa agataagaag ttacatcacc ttacgtctaa acatcgcgta cgccgctgac
    18781 cctgtcgtgg ccgttatcac cagtagcggc cacaaactat tccacacaat atccagactc
    18841 gcatccttca aataaatctg cttggtaatg tccgtaaagt ggcgaatagg gttaatccac
    18901 gtcaggtttt gcagccatac tggcatgttt tccaccggag aaacgtaacc ggaaaggaga
    18961 atggcgggca tcataaaaac aaacacgccg ataaacgcct gctgttgtgt cgagcagagt
    19021 gatgaaatca acagaccgaa tcccaccagc gataaaccgt aaatcaccat cgtaaagtag
    19081 aacagcgcca gcgatccggc gaaggggatt tgatacgccc agataccaat cgccagcaca
    19141 atggtggcct ggaaggtggc gacaattaac gccggtacgg ctttgccgat gaagatctgc
    19201 caggtggtga gcggcgaaac cagtagctga tcgagcgtac cttgttcacg ttcgcgggcg
    19261 acggaaagtg aagtgacgat cattacgccg atagtggtga tcatggcgat cagcgacggc
    19321 accacaaacc atttgtagtc aagattcggg ttataccagt tgcgtaccac cagctcgctg
    19381 ttgttaggtt tcggttttcc ttccagcagc tcctgctgat aatttttgac gatctgttgc
    19441 aggtagttgg cggcaatttg cgcactgttg gagttacgcc cgtcgaggat caactgcaac
    19501 ggcgcggtct ggaaggtatc cagtttgcgc gagaagtcag ccgggaaacg caccagtagt
    19561 aacgcctttt gtgtgtcgat ggttgggcgg atctcctgtg ggcttttcag cagcagcaca
    19621 tgggtaaagg cgctggcgcg ggcaaaacgt tgggtcagct ccaccgaatg ctcgccgtta
    19681 tcttcatcgt agatggcgat ggtggcgtta gtcacttcca gcgtggcggc gaacgggaac
    19741 aggatcacct gaataagcac aggtaaaatc agaatcgcgc gggtttgtgg ttcgcgcagc
    19801 aacgactgca actctttgcg gattaacgtc cataagcgat gaaacatgct cttctcccta
    19861 atccagccga cgtttggttt tcagccacgt caggccgata aacatcaccg ccgaagcgat
    19921 caaaaacagc acgtttacca ccagcaccac tggaatattc ccggcgagga acaggctttg
    19981 cagggtgctg acgaaataac gagcgggaat aatgtacgtc accgcgcgga tcaccgcggg
    20041 catactgtcg atctgaaaaa taaagccgga aagcataatc gacggcagga acgcggcgtt
    20101 cagggcgacc tgagcggcat tgaactggtt gcgggtaatc gtggaaatca gcagccccat
    20161 ccccagggta ctgagtaaaa acaggctgga gataaaaaac agaataagca gcgaaccgcg
    20221 atacggcacg ccgagaataa acactgacac cagcatacac agcagcatcg ccagcatccc
    20281 gagaaagtaa taagggatca gcttacacag cagcagttcc gtgcgggtaa tctccgtaga
    20341 gagcagagcc tccatggtgc cgcgttccca ttctcgcgcc accaccagcg aggtgagaat
    20401 cgcgccgatg accgtcatga tgatggtcac tgcaccgggg ataatgaagt gctggctaat
    20461 cgccgccggg ttaaaccagt agcgggtttg tacatcaata agcggttcaa aagtctgccc
    20521 gttgtcctcc gctcgctgca tttgccagat ctgccagatc tgccagatcc cttcgacata
    20581 cccctgtaca aagttagcgg tattcggctc actgccgtcg gtgatcacct gaatcggtgc
    20641 ggtggcgttg gcgcgctcca tctgttccgc aaaatccacc ggaataacca ccagaccgcg
    20701 aattttcccc gcctgcattt tggcgatcag ttcctgacgg ttatcgctga tggtggcgtc
    20761 gatgtagggc gaaccggtca tggtgtgggt gaaatccagc gccgcttcgc tacgctgttc
    20821 cagcaagatc ccgacccgca gcttgctgga gtcgaggtta atgccataac caaaaataaa
    20881 cagcagtagc agtgggatca ctaccgcaat cagccagcta ctcggatcgc gaacgatctg
    20941 ccgcgtctct ttgacgcaca gcgcccgcac gcgacgccag gaaaggatcg ggttactcat
    21001 tgctatgctc cttatcccag tcgtggatca actgaataaa ggcttgctcc atggtgggat
    21061 cgggttgctc atcgttagcc gactgtgctt tcaaatcgtc cggcgtaccg ctggcgatta
    21121 atttcccacg gtacaccagg ccgatgcggt cgcaatattc cgcttcatcc ataaagtggg
    21181 tggtgaccat caccgtgacg cctttctcta ccatgctgtt gatatgtagc caaaattcac
    21241 ggcgggtgag gggatcaacg ccggacgtcg gttcgtcgag aaacagaatg tccggttcat
    21301 gcatcagcga acaggccagc gccagccgct gtttaaaacc taatggcagt tcatcggtgg
    21361 cgtgggaggc gatacttttc aggccgaacg cctcgctcat gcgggagatt ttttcgttct
    21421 gcgcccgacc gcgtaagcca tacacaccag agaaaaagcg taaattctgt tcgaccgtca
    21481 ggttaccgta gagcgaaaac ttttgcgcca tatagccgag atgctggcgc gctttaccag
    21541 aactctcttt cagatccatc cccagtacca gcgcctgacc agaggtcggc accagcaaac
    21601 cgcacatcat cttaaaggtg gtcgatttgc ccgcgccgtt tggccccagc aaaccaaaaa
    21661 tctccccgcg tttaacggca aagttgacgt gatcggtggc ggcaaaatca ccaaatttct
    21721 tggtcagttc tttcgcttcg atcaccgtct cgccgggtgt gccttctacc gtatgtaata
    21781 ttgcgcccag cggcgattcc gaggttccgg caccgcccag caaatcaata aacgcatctt
    21841 caaaacgcgg cgtagtttcg ttgatgttga tttccggcat cccgtcggca tggcgaatat
    21901 cgtctggtgt ggtctctttt ttgaggatca gacgtaccga tttcccctga atcatgccgt
    21961 cgctgacctg cggcagtttc aatgcccgtt gcaacagttt gcggttgccc tcgtgtggac
    22021 ttgtcatcag aaagctgcgt ccggccatgg tttgtgtcag ggcttttggt tccccctgat
    22081 acagcaactc gccttcattc atcagtaaca cgtcacggca ctgctcggct tcgtcgagat
    22141 acgaggtact ccagaggatt aacatccctt cgcctgccag ctcatgcacc atctgccaca
    22201 gttcgcgccg tgagataggg tcaacgccga cgccgggttc atcgagcagc aacactttcg
    22261 gttcgcccac cagggtacag gccaggccga gtttttgttt catcccaccg gagagcttgc
    22321 ccgccaggcg tccggtaaac ggcccaagag acgtaaactc cagcaggcga gcaaaagttt
    22381 gcttacgtgc ctcgccggtg acgctgcgca aatccgcgta cagattgagg ttctccatca
    22441 ccgtgagatc ttcatacaga ccaaatttct gcggcatata gccgagcacg gcgtgcagcg
    22501 cgccgtcgtt tttgattgga tcaaagccaa tcaccgtggc attgccgctg tcgggtttca
    22561 gtaatcccgc cagcatccgc attagcgtgg ttttacctgc accatccggc cccaccagcc
    22621 ccgtcacata accggcgtga atggtacaat cgagcggcgc gacggcgggc ttgtccatgc
    22681 ccggaaagcg tttttccagg ccgttcagcg tgataacggc atcattcatg tcctgcctcg
    22741 ttaccgaatt gcaccgtcac cggcattccc tggcgtaacg catcatcggc gtcggtcacc
    22801 acaatacgca ggcgatagac gaggtcggta cgcagatccg gtgtttcgac ggttttcggg
    22861 gtaaattcag cagtcggcga aacgaaacca atctgcccgt gatacggctt gtccgggcga
    22921 ccatcggtat aaagcagcac tttgcgcccc ggctgggcct ggtcaagata ccgttcatca
    22981 acataagcgc gcacccacac cggacgcgtt agtgaaacgg taaacaccgt gccaccttca
    23041 ttgaggaccg tgcctggctc caccgcgcgc gttaacagcg tgccatcaga cggggctatc
    23101 aacgttgagt cctgtaaatt caactccgcc tgcgccagtt gcgcctgcgc ctgttcgagg
    23161 ctggctttcg cctgggcgat gtcctgttca cggttaccgg aacggtactg acgcaattta
    23221 tcctgtgctg atttcagcgt tgcctgcgcc tggtcgcgcg aggagcgggc attttccagg
    23281 tcatttgccg aaatagtgcg gcttttccac agtccttgct ggcggttata gaagttctgc
    23341 gcatagtcat aggcggcttg cgcctgtttc accgctgcgg cggcctgagc gatttcttca
    23401 tcgcgatacc cggcaagcat cagatcatac tgcgcctgtg ccaccgagac gcccgctttc
    23461 gcctgcatca gggcaatctc atacggcttg tgatccagtt cgcccagcac ctggcccgct
    23521 ttgatagcat caccttcgtc caccgccagc gattcaacgc gccccccaac acggaaacta
    23581 agattcaccg tacgaatatc cacgttgcca tacagcgtca ggccgttatc ctggcggctt
    23641 tgataccacc agtagcctcc ggcaaccacg gcggcaagta ccactaccgc cagttcgatc
    23701 acgacaggtt ttttcatcac tacaaactcc tttgcgataa tccttgcaaa atcaggtcga
    23761 tatgacaggt caccgtctgg ttgatcagtt cggtcttttc ttcatcgaac gcggtccagc
    23821 cggtacgtaa cagaattgtt tctttgccaa gacggaacgc cagaatctca ccaatcaacg
    23881 catgggtatg aaggatcatg cgggtgtcat tggcgtcgct gccggtccag gcggcaatca
    23941 ggcgtgtcag gtggctgtgt agcggactaa tcacctgttc gtgcaccagg tggtaggctg
    24001 ccgtgggaga gagctgctca cgggagataa acttgctgag gttgacggtg tcatcctggg
    24061 tgagcagctt aatcatgttc ctgcaggcgc gaaggagcag ttcacggatg gcagcccgat
    24121 caggctgtgg ttgggcgaac aagcgttcgg cttcctcggc atgcggacgg aactgctcgc
    24181 caataaaatc ggcaatccac tgggcgcagg cgaggtacaa atcttctttc gaaccgaagt
    24241 agtaggtgat ggcagcgata ttctgcccgg cttgggcggc tatctcgcgg gtggtggcgt
    24301 tcattccata ttcaccaaac tgcgccagtg cggcagcaat cagctgtttt ttcgcctgtt
    24361 cacccttgat tgtcatggca ggattattca tcgcacagtc cattcttaat caaatgattg
    24421 attaagatta tgactccata tgggagttgt ccagtatggc taagaatttt agctacgcca
    24481 gtcacaggga taatttatgc gctgcgtcac aaaaactgct acactccgct cgctcatgac
    24541 attgtggttt ttgtcatttt ccttttcagt atctccctga aaactacacc ggtaacggtc
    24601 ggggcggttc ggagtagtca tgtctttcga ttatttgggt ttaagccctg atatcctgcg
    24661 cgccgttgcc gagcagggtt accgtgaacc cacccctatt cagcagcagg cgatccctgc
    24721 ggtgctggaa ggccgcgacc tgatggctag cgcccagacc ggcaccggca aaacagcggg
    24781 ctttacgctg ccgctgttgc aacacctgat cactcgccag ccgcacgcca aagggcgtcg
    24841 tccggtacgt gcgctcattc ttaccccgac ccgtgaactg gcggcgcaga ttggcgaaaa
    24901 cgtccgtgat tacagcaaat acctgaacat tcgttcgctg gtggtgtttg gtggtgtgag
    24961 tattaacccg cagatgatga aactgcgtgg cggcgttgat gtgctggtgg caacgccggg
    25021 acgtttgctg gacctggaac atcagaatgc agtgaagctg gatcaggttg aaatcctcgt
    25081 cctcgatgaa gctgaccgca tgctcgacat gggctttatc cacgatatcc gtcgcgtgtt
    25141 aacaaaacta cctgcgaagc gccagaacct gttattctcc gcgaccttct ctgacgatat
    25201 taaagccctg gcggaaaaac tgttgcacaa cccgctggaa atcgaagtgg cacgccgcaa
    25261 taccgcgtct gatcaggtga cccagcacgt tcactttgtc gataagaaac gcaaacgcga
    25321 attgctgtcg cacatgattg ggaaagggaa ctggcagcag gtgctggtgt ttacccgtac
    25381 caaacacggc gctaaccatc tggctgaaca gctcaataaa gatggcatcc gtagtgcggc
    25441 gatccacggc aataaatcgc aaggtgcgcg tactcgtgcg ctggctgatt ttaaatcggg
    25501 cgatattcgt gtactggtgg caactgacat cgctgcgcgc ggcctggata ttgaagagct
    25561 gccgcacgtc gtcaactatg aactgccaaa cgtaccggaa gattatgtcc atcgtatcgg
    25621 gcgtaccggt cgtgcggctg ctaccggtga agcgttgtcg ctggtgtgtg ttgatgaaca
    25681 caaactgctg cgtgatatcg aaaaactgct gaaaaaagag atcccgcgca ttgcgattcc
    25741 gggctatgag ccggacccgt caatcaaagc cgaaccgatc cagaacggtc gccagcaacg
    25801 tggcggcggc ggtcgtgggc aaggtggtgg tcgcggtcaa cagcaaccac gccgtgggga
    25861 aggtggcgca aaatctgcaa gcgcgaaacc tgcagaaaaa ccgtctcgcc gcctcggcga
    25921 tgccaaaccg gcaggcgaac aacaacgtcg ccgccgtccg cgtaaacctg ccgctgcgca
    25981 gtaatctttt atgccgggct atgcccggca tcaggctgat gaacaaacgc aaaactgcct
    26041 gatgcgctac gcttatcagg cctacgtgaa ctctgcaata tattgaattt gcatgctttt
    26101 gtaggccgga taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa
    26161 tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact
    26221 tctctatagc caattgttct cgcaactcca ttaaaaggta gcccagtcga ttcttgccct
    26281 taccattacc accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg
    26341 gcgcggttgc cagcaagagc acacgcagtt ctgcatgctg ttcgaattta gcacgaagtg
    26401 ctttacgcat cacctgttct ttaaccgact cccagttttt acgaagtggc ttggaacgat
    26461 tacgccccat gcgagcggcg accatggggg aagaaacccg acggatctct tcgcggtatt
    26521 tttcatcaag gaatttttgt gcctgaaaat agtgttcgga ggtgggccag gttttcccgt
    26581 caaccttgat gggccaggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt
    26641 agaaatttat gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta
    26701 aaccgcatta tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc
    26761 ctgaaaaccg aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg
    26821 gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag
    26881 atccccgact ttattccccg tgtgccgcag cggcagatga ttgcggacgt cgccaaaacg
    26941 ctggccggag aagaagggcg gcatctggcg attgaagccc ccaccggcgt tgggaaaacg
    27001 ctctcctatt tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg
    27061 agtaccgcca acgtggcgtt gcaggatcag atttacagca aagatttacc gctgctgaaa
    27121 aagatcattc ccgatcttaa attcactgcc gctttcgggc gtgggcgcta cgtttgtccg
    27181 cgtaatctga cggcgctcgc cagcactgaa cccacgcaac aggatctgct ggcgtttctt
    27241 gacgacgaac tgacggcaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc
    27301 gatctcgaca cttatagatg ggatggtctg cgcgatcata cggatatcgc tatagatgat
    27361 gatctctggc gtcgtttgag taccgacaaa gccagctgcc tcaaccgtaa ctgttactac
    27421 tatcgtgaat gcccgttttt tgttgctcgt cgggagattc aggaagcgga agtggtggtg
    27481 gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag ccgtactgcc tgacccgaaa
    27541 aatctgctgc tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg
    27601 gagatgagcg ctgaaatcac cgcgccctgg tatcgactac agctggactt gttcacgaaa
    27661 ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct
    27721 gaacgtttga atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt
    27781 ctcaatctct acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg
    27841 cctgatgaag tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt
    27901 ctggcggagt tatttcttaa cgatttaagt gagaaaaccg gcagccatga cattgtacgc
    27961 ctgcatcggt tgattttgca gatgaaccgc gcgttgggga tgttcgaggc gcaaagcaaa
    28021 ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg
    28081 cgggaagagc gcgaagggca actgcatctc tggtttcact gcgtggggat acgtgtcagc
    28141 gatcagctgg aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc
    28201 ttgcgttcgc tgaatagttt ttcgcgtttg caggagatga gtggtctgaa agagaaagcg
    28261 ggcgaccgtt ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt
    28321 attccccgga tgcgcgttga gccttccatc gacaacgaag agcagcatat tgccgaaatg
    28381 gcggcctttt tccgtaagca ggtggagagc aaaaaacatc tcggtatgtt ggtactgttt
    28441 gccagcggac gggcgatgca gcgctttctc gactatgtga cggatttacg tctgatgttg
    28501 ctggttcagg gcgatcagcc gcgttaccgt ttagttgaac tgcaccgcaa acgcgtcgcc
    28561 aacggtgagc gcagcgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa
    28621 ggtgatctgc tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccc
    28681 gtggtgatca ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa
    28741 agcctgccga gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac
    28801 ggttgctggg gcgaagtggt tatctacgac aaacgcttgc tgaccaaaaa ttatggcaag
    28861 cgactactgg atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata
    28921 gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca
    28981 ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaaa
    29041 cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt atgctcatat gctcaatggt
    29101 gaagtccctg accttgagtt gggcggcgta ttgattgcgc tgcgtatcaa aggagaaggg
    29161 gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg
    29221 ccagcaggca agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggcc
    29281 aacctgacgc cgttgttggc gattctcctg cacaaactcg gttttcctgt ggtggttcac
    29341 ggggttagcg aagatccaac ccgcgtgctg actgaaacca tttttgaatt gatgggtatt
    29401 acgccaacgt tgcacggcgg gcaggcgcag gcgaagctcg acgaacatca accggtgttt
    29461 atgccagtcg gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg
    29521 ggcgtgcgta acagtgcaca taccctggcg aaactggcga cgccatttgc cgaaggtgcg
    29581 gcgctgcgtc tttccagcgt ttcgcatccg gaatatattg gacgcgtcgc gaagttcttt
    29641 agcgatatcg gcgggcgggc gctactaatg catggtactg aaggtgaagt gtatgctaat
    29701 ccgcagcgct gcccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa
    29761 aaacaggaca ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg
    29821 cagtggattg agcgttgcct tgctggcagc gaaccgattc ccgaatcgct gaaaatccag
    29881 atggtttgct gcctggtggc tatgggtgaa gcggcaacta tcagcgacgg cctggcgcgc
    29941 gttaatcagg cattttaatt cttttctcag ccggatgagc catgtcatcc ggcttttacc
    30001 ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc cacgcccggc gtgcagcata
    30061 aaaatacaac aaacacataa cataaacagg agttaaccat ggaaagtggt catcgctttg
    30121 atgctcagac gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac
    30181 aagaagcgaa attagttgcc gatcatttaa tcgcggcaaa cctggcaggg catgattcac
    30241 atggtattgg catgatccca agctatgtac gctcctggag tcaggggcac ctgcaaatta
    30301 accatcatgc caaaaccgtt aaagaggcgg gggcggcggt cacgctcgat ggcgatcgcg
    30361 catttggtca ggtcgcggca catgaagcga tggcgctggg gattgagaaa gcgcatcagc
    30421 acggtattgc cgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg
    30481 cggagcagtg tgcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc
    30541 caatggtcgc gccgttccac ggtcgcgaca gccgctttgg caccaatccg ttctgtgtgg
    30601 ttttccctcg taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat
    30661 ttggcaaaac ccgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg
    30721 acgttaacgg cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttgggttcgc
    30781 tgttgacctt tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg
    30841 gggcgctttc cggcggtaaa acgacgcatc aggaaacgtt acaaaccagt cccgatgcca
    30901 ttcttaactg catgaccact atcatcatca acccggaact cttcggcgcg ccggattgta
    30961 acgcgcagac cgaagccttt gccgagtggg tgaaagcctc gccacatgat gatgataagc
    31021 cgattttgct accgggcgag tgggaagtga acacgcgtcg cgaacggcag aagcagggga
    31081 ttccactgga tgcgggaagc tggcaggcca tttgtgatgc agcgcggcag attggtatgc
    31141 cggaagagac gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag
    31201 tggcggacgg gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt
    31261 cggcaaacag acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac
    31321 ggttcggatt ttgtaggcct gataagacgc ggtagcgtcg catcaggcat ctgttgccgg
    31381 atgcggcgta aacgccttat ccgtcctacg aattacttat aaattaccgc agtaccgctt
    31441 aatttattgt tgttggtggc ggaagtgatg ctgtatccac tggcaccggc tgccgcggct
    31501 ttctcagcca gtttcgcttc cagggcatcg agggtggatg cgccatcggc agaaaccacg
    31561 ccgattttat tcatgttctg tgcctgggat gccgttatcg gttccgccgc gaacacgcca
    31621 aatgacagag ttgaaagagc catagcagca acaacagtat tgatagtttt cataattaat
    31681 ctctcgcagg tgatttttgt ataagtgacg ttgtttcgtc gatgtgatga gtatcacgtt
    31741 tttttacgag agataaaatc gaagagaatt gaccgccttg ttcaaataaa ttgattgata
    31801 tctaacatac tgataaacca tttatttgtt gtacttatga acttgtttta taccgcgtgg
    31861 cagtcacagc agcgttaaag gcacggaatg ccacgcaaaa taaaaattat actcgctatg
    31921 catctcgcca gttgtagtta acgtaactgg ctgtctttcg aacctctgcg attatatcct
    31981 gtataagctg gtttttgtaa atctttctcc tgctgacaat gaatacataa gcgcacgcca
    32041 ggaatggctt cccgacgggc ctgcgggatg ggggcaccgc actcttcaca ttcatccagg
    32101 ctttcgccgc gcggaatttc accccgagcg cgggcaatcg cattttcaat tgtactgttg
    32161 atctgttcgt tgacggcgtc atcgttagcc caaccggatg ccatatcgac ctccccatat
    32221 caatacttgt acagttaagt gtagctaatc cagggacgaa ctcgggcagt tcaagcatca
    32281 gatctccgac cattcccgca gcagattatg ataaagatta agcagcgaca ggatctcttc
    32341 actttcgccg tagcggcttt tcagcgactg aatattgttg tccagttcaa acagcatggc
    32401 gcgcttttta tcatcgcgga tcatcgactg gatccacata aatgatgcca ctcgtacgcc
    32461 gcgggttacg ggtggcacgc aatgctggct gctggaggga tacaacacga gatcgcctgc
    32521 cgggagtttt acccgatgtt gtccgaaggt gtcattaacg accagttcgc cgccgtcgta
    32581 gctttgtgga tcgcttaaaa acagcgtggc agaaaggtca gtacgcatcc agccgttttg
    32641 tggatggctg cgtactgcgc catccacatg gaaaccatag gtttcattgt tctgatagcg
    32701 attaaacagc ggcgtggaaa gggtacgcgg caaggccgcg gcaaagaaca aagcatgttg
    32761 gttaaccgcg ttcagcacct cattttgcag ggcggcgtat aacgtgctgc gggtgtcgac
    32821 ctgttgattg ttcttaactt gcgcaccttg tgcgccggtg gtgacgcgtc catccaccca
    32881 ttcggcttgt tccagttgtt cgcgaaaccg ggcgacgtcc tgtggcgata acacgccggg
    32941 aatgtggtac atcatacaag ttctccaaaa agtggggcct gcgccccaca tctgaatcag
    33001 aaatgcatat tggctgtgag caagaaggtt cttggctcgc ccgggtgata acggtagccg
    33061 ctcttgttga ttgaggcgac gtaatcagta tcaaacaggt tgtagacgtt tagctggaag
    33121 tcgagattgc gattaacccg ataccccagt ttggcatcgg cgacccagta accttcggta
    33181 aatgctggcg ttcccaccgc gccgtctgaa cctttatgca tactgccgat atagcgtgcg
    33241 cccgcgccaa cagagatatc gtcggttgcc tgatattggc tccataaggt gaaggcgtgc
    33301 tccggggtat acggcagcga tgaggaacca tcctgggcaa catctttgcc gtttttgatg
    33361 gttgcttttt gctgggtata gccgccaatc acctgccacg cgggagtgat attcccggcc
    33421 acggatatct catagccttc gacgcgtttc ttaccgtatt gcgagtaagt tccgtcatca
    33481 ttttgctcaa cttcattttc gatatcagta cggaacagcg cggcggtgag caacagacgt
    33541 ttatccagaa cctgccattt ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa
    33601 tcggtgcggt tggcactgtt accgctgcca gactgcgcaa gggcgaagtt gttgccgccc
    33661 ggaggctgct gggaaacggc atagttaata tagacattgc cgttttccgt caggtgatac
    33721 agcgccccgg ctttccagtt catcagattg cccgacttgg cggtgtcgac ggtggtgacc
    33781 ggagaacctt ttgccacacc agttgggcag gtgatggcac cgcgtccgct gccgccgcag
    33841 gcggtggcac tgtcatattc agtatgataa ttatccagac ggatcccgcc gttcagctca
    33901 aaatcacggg tgatttgcag cgtatcaaag gcgtaaattg cgaaggtatc cgtctgacca
    33961 ttggcgtttg cgccgttgcg cgtcaggccg ccgggatgaa tgctgctgtc aggatgataa
    34021 atatttaccg cgggtaacgt caccggatta acgccgtagt tagtctgcgt ttcacgggta
    34081 aattccacgc cggtactgac atcatgaccg atagaaccgg tatagaacgt cgaggtcagg
    34141 ttggtctggt tggtgagaat tttattactc acatctttgg tattcgccgt gcgtgaccag
    34201 gttcagctat tcacatcgct ggtgggctga gtaatattcg acgccccgcc cataatcgcc
    34261 gtcatcaggt aatcctgctt tacgcgcgac caacgggtag tattgcgaat ggtggtgtta
    34321 tcgttgatgt cgtgctcaaa acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg
    34381 gaatccgtgc cgtaaaagtt atgagtatca acttttccgg aatgattcag ggccgccgtt
    34441 cccgcagatg gggcagaata gcccggcaaa ccgatggtcg gaatgccgcc gtctggcgtg
    34501 ttgtgctggg tgacatgcag ataattaaga tacaaacgat tcgctgtacc aaggccaaaa
    34561 gcgacagaag gggcgacgcc gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgc
    34621 gttttttcgc ccattacatt caggcgcacc gcggtggtat caccaatgac ctgattgacg
    34681 tccagcgtgc cgcggcggaa ccaggcgctg ccaatactgg cggaggcgtc aatgccggaa
    34741 tcattgcgcg gctgtttgct gatcatattg atcgagcccg tcggtgcgct gcgcccgtag
    34801 tcggtgccgg acggcccttt aatcacttcg acctgctcgg tattgaaggt gtcgcgcgag
    34861 acgctgccga tatcgcgaat gccatcaata taaatactgt tagaggtatc ggcaccacgc
    34921 atataaatgg cgtcgccagt ggtggagtta ccgttctcac ccgcaaaaaa cgcacccacg
    34981 c
//
LOCUS       DAESHI010000036        34771 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.036, whole genome shotgun sequence.
ACCESSION   DAESHI010000036 DAESHI010000000
VERSION     DAESHI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34771
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.036"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(209..3313)
                     /gene="acrF"
                     /locus_tag="KW011_001793"
                     /pseudo
     CDS             complement(209..3313)
                     /gene="acrF"
                     /locus_tag="KW011_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(3325..4482)
                     /gene="acrE"
                     /locus_tag="KW011_001794"
     CDS             complement(3325..4482)
                     /gene="acrE"
                     /locus_tag="KW011_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HBH7660139.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            4881..5543
                     /gene="acrS"
                     /locus_tag="KW011_001795"
     CDS             4881..5543
                     /gene="acrS"
                     /locus_tag="KW011_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HBH7660140.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(5546..5725)
                     /locus_tag="KW011_001796"
     CDS             complement(5546..5725)
                     /locus_tag="KW011_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HBH7660141.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(5809..6693)
                     /gene="yhdJ"
                     /locus_tag="KW011_001797"
     CDS             complement(5809..6693)
                     /gene="yhdJ"
                     /locus_tag="KW011_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBH7660142.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(6779..7075)
                     /gene="fis"
                     /locus_tag="KW011_001798"
     CDS             complement(6779..7075)
                     /gene="fis"
                     /locus_tag="KW011_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HBH7660143.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(7101..8066)
                     /gene="dusB"
                     /locus_tag="KW011_001799"
     CDS             complement(7101..8066)
                     /gene="dusB"
                     /locus_tag="KW011_001799"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBH7660144.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(8395..9276)
                     /gene="prmA"
                     /locus_tag="KW011_001800"
     CDS             complement(8395..9276)
                     /gene="prmA"
                     /locus_tag="KW011_001800"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBH7660145.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(9288..10739)
                     /gene="panF"
                     /locus_tag="KW011_001801"
     CDS             complement(9288..10739)
                     /gene="panF"
                     /locus_tag="KW011_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HBH7660146.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(10729..10971)
                     /locus_tag="KW011_001802"
     CDS             complement(10729..10971)
                     /locus_tag="KW011_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HBH7660147.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(11080..12429)
                     /gene="accC"
                     /locus_tag="KW011_001803"
     CDS             complement(11080..12429)
                     /gene="accC"
                     /locus_tag="KW011_001803"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBH7660148.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(12440..12910)
                     /gene="accB"
                     /locus_tag="KW011_001804"
                     /gene_synonym="fabE"
     CDS             complement(12440..12910)
                     /gene="accB"
                     /locus_tag="KW011_001804"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBH7660149.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(13888..14862)
                     /locus_tag="KW011_001805"
     CDS             complement(13888..14862)
                     /locus_tag="KW011_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBH7660150.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            15014..16954
                     /gene="csrD"
                     /locus_tag="KW011_001806"
     CDS             15014..16954
                     /gene="csrD"
                     /locus_tag="KW011_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HBH7660151.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            17259..18302
                     /gene="mreB"
                     /locus_tag="KW011_001807"
     CDS             17259..18302
                     /gene="mreB"
                     /locus_tag="KW011_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HBH7660152.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            18368..19471
                     /gene="mreC"
                     /locus_tag="KW011_001808"
     CDS             18368..19471
                     /gene="mreC"
                     /locus_tag="KW011_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBH7660153.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            19471..19959
                     /gene="mreD"
                     /locus_tag="KW011_001809"
     CDS             19471..19959
                     /gene="mreD"
                     /locus_tag="KW011_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBH7660154.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            19968..20561
                     /gene="yhdE"
                     /locus_tag="KW011_001810"
     CDS             19968..20561
                     /gene="yhdE"
                     /locus_tag="KW011_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HBH7660155.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            20551..22020
                     /gene="rng"
                     /locus_tag="KW011_001811"
     CDS             20551..22020
                     /gene="rng"
                     /locus_tag="KW011_001811"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HBH7660156.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            22088..25888
                     /gene="yhdP"
                     /locus_tag="KW011_001812"
     CDS             22088..25888
                     /gene="yhdP"
                     /locus_tag="KW011_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HBH7660157.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            26044..27489
                     /gene="tldD"
                     /locus_tag="KW011_001813"
     CDS             26044..27489
                     /gene="tldD"
                     /locus_tag="KW011_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HBH7660158.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(27617..28546)
                     /gene="aaeR"
                     /locus_tag="KW011_001814"
     CDS             complement(27617..28546)
                     /gene="aaeR"
                     /locus_tag="KW011_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HBH7660159.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            28729..28932
                     /locus_tag="KW011_001815"
     CDS             28729..28932
                     /locus_tag="KW011_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HBH7660160.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            28940..29872
                     /gene="aaeA"
                     /locus_tag="KW011_001816"
     CDS             28940..29872
                     /gene="aaeA"
                     /locus_tag="KW011_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HBH7660161.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            29878..31845
                     /gene="aaeB"
                     /locus_tag="KW011_001817"
     CDS             29878..31845
                     /gene="aaeB"
                     /locus_tag="KW011_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HBH7660162.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            31937..32209
                     /locus_tag="KW011_001818"
     CDS             31937..32209
                     /locus_tag="KW011_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HBH7660163.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(32265..32528)
                     /gene="yhcN"
                     /locus_tag="KW011_001819"
     CDS             complement(32265..32528)
                     /gene="yhcN"
                     /locus_tag="KW011_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HBH7660164.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(32893..33363)
                     /gene="argR"
                     /locus_tag="KW011_001820"
     CDS             complement(32893..33363)
                     /gene="argR"
                     /locus_tag="KW011_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HBH7660165.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            33798..34736
                     /gene="mdh"
                     /locus_tag="KW011_001821"
     CDS             33798..34736
                     /gene="mdh"
                     /locus_tag="KW011_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBH7660166.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT         8495 a         8637 c         9073 g         8566 t
ORIGIN      
        1 tcttattaac gagctgaatg ccattttatc accggaggtt ataaatattg cggaatgaat
       61 aatgacaaat tatgtgaatt gtaaaaatag tgaagggagc gggaactata gaaagttttt
      121 aaagagtgag tagaagaata tattattaat ttttatgggt ttcactgaaa ataataaagg
      181 cacccgaaag tgcctttatg tttctgattt atcctttaaa gcaacggcgg atcaccacaa
      241 agaacaccgg tacgaagaag attgccagca acgttgcaga gaccattcct cccattaccc
      301 cgatacccac tgcgttctgc gcgccactgc cggcaccgtt actgatagct agcggtaata
      361 cgccgagaat aaaggcgaga gaggtcatca ggataggacg cagacgcata cgtactgcca
      421 tcagtgtcgc ttcaacaaca cctttaccct ttttctccat gagatcttta gcgaactcaa
      481 caatcaaaat agcgtttttg gccgacaagc caattgtcgt tagcaagccc accataaagt
      541 agacgtcatt tttttgatta aagagtgttg ccgccagtag cacgccgaca atccctaacg
      601 gcacgaccaa cataaccgag acaggaattg accagctttc atagagtgca gcaaggcaca
      661 ggaagacaac cacaaaggaa attgctacca gagcgggagc ctggtttccc gataagcgtt
      721 cctgatacga catacctgtc cagtcataac caatgcccgc aggtaatttt gacgcaaggt
      781 tttccatcaa tgccatggca tcgccggaac tggttcctgg cgcggcttcc ccctgaatct
      841 ccattgacgg cagaccgttg tagcgttcca gtcgcggaga gccatacacc caatgtgaag
      901 tggtaaaggc agagaatggc accatttcgc cgttggcgct gcggacataa agtttatcga
      961 catcttctgg cagcatacgg aatttggcat ccgcctgaac atacaccttt ttcacgcgac
     1021 cacggtcgat gaagtcgtta acgtaagtcc cacccagcgc cgttgaaatg gtctgattga
     1081 tgtcagaaag tgaaacacct aatgcctgcg ccttttcctg gtcaacttcc agtttaaact
     1141 gcgcggtgtc ttccagacca ttaggacgca cgctgactaa gctggcagga tgttgcgccg
     1201 ccatgccaag caactggtta cgggcctggg tcagggcatc gtgacccagc ccagcctgat
     1261 caattaactc aaagtcgaaa cccgttgccg tgcccagttc aacaatggct ggcatattga
     1321 atggaatgac aaaaccgtcg cggatcttgc ccaattccat tttggcacga tggattaccg
     1381 cttccgcact gttttcgtca ccactacgct cttcccacgg tttcagactg acgaaagcca
     1441 taccggcgtt ttgtgcctgg ccgctgaagc taaagccgtt aaccgtaaag acactttcta
     1501 cgttcgcttt ctcgttattc agatagtaat ccgtcacttg atccaacact ttttgcgtcc
     1561 gctcttgcgt cgcgccagcg ggtaactgaa tcatggtcag aaagacaccc tgatcctctt
     1621 caggtaagaa ggaagacgga agacgtaaaa acaacaccac cattcctgca acaatcagcg
     1681 catagatcag taaatatcgt cctgtggaac cgaggatttt gccgacgctg ttggtgtagt
     1741 ggttaacgct atgatcgaag gtggtattaa accagccgaa gaaaccgccc ttattttcgt
     1801 gatgctcagc agagacgggt ttaagcagcg ttgcacataa cgcaggggta agaatcaatg
     1861 ccaccagaac agaaagcgcc attgccgaaa cgatggtgat agagaactgg cgataaattg
     1921 ccccagtaga accgccgaag aatgccatcg gaataaatac cgctgacagc accatcgcga
     1981 tacccaccag tgcgccctga atttgcgaca tcgatttttc cgtcgcttct tttggcggga
     2041 gcttatcctc catcatcacg cgctcgacgt tctccaccac cactatcgca tcatcgacga
     2101 gcagcccgat ggcaagcacc atcccgaaca tcgttagtgt gttgatggag taaccaaaag
     2161 cggcgaggat ggcaaacgtc cctaacaaca ccacgggtac cgcaatggtg gggatcagcg
     2221 ttgctcgcat attctgcaag aacagataca tcaccaggaa caccagcata atggcttcga
     2281 acagcgtttt taccacttcg tgaatagaaa gctggacgaa tggcgtggtg tcataagggt
     2341 agagaacctt cattccctgc gggaagaatg gctgtaattc cgccagtttt gccttaatgg
     2401 ctttcgcggt atcgagagcg ttcgcgccgg ttgctagctt aatccccagg cccgccgccg
     2461 gttttccgtt gatacgagcg ataacgttat agttttcacc gccaagttca actcgtgcga
     2521 catctttcag gcgtaccacc gagccgtcac tgtttacgcg cagggttact ttgccgaatt
     2581 cttccggatt ttttaaccgc gtctgagcaa taatcgaggc gttcaactgt tgccctggta
     2641 acgctggcgt tccgcccaac tgcccggcag cgatctgatc gttctgtacc ttcaactggt
     2701 taatcacatc aaccggtgtc agtttatatt tgtttagcag atcggcgtcc agccagatac
     2761 gcatcgcata ctgtgcgccg aaaagctgta cgtcaccgac gccattcaga cggctaagcg
     2821 tatctttaac gttagaggcc acatagtccg agatatcgtc ctgtgtggtg tctgggttat
     2881 cagagacaaa gcccgccacc atcaaatagc tgctactgga cttttcaaca ctgatcccct
     2941 gctgctgaac ctcctgcggc agcaacggcg tggcgagctg gagtttgttc tgcacctgca
     3001 cttgcgcgat atcaggatcg gtcccggact ggaaggtaag ggtaattgtc acgctaccgg
     3061 cggaatcgct ggtggaggac atatacatca ggttatcgat accattcata ttctgttcga
     3121 taacctgcgt caccgtatcc tgcacggtct gcgcatcagc acctggatag tttgcctaaa
     3181 cagaaaccgc aggcggtgca atggttggat actgagcgac gggcaattgt aggatcgcca
     3241 gtgcgcccgc catcatcaga ataatggcca gcacccatgc aaatatcggt cgtcgaataa
     3301 aaaagtttgc catgtcagat taccttactt cgatgcagta tctgcggggg tatcggtagt
     3361 ggctttaacc tgctctcccg gacgcgcttt ttgcaggccg ctgacaatga cttgatcgcc
     3421 agatttcaga ccttcgctaa tcaaccattt atcgccaatc gcctgactgg caacgacagg
     3481 gcgcgcttcc acctgacttt tatcgttaac aatcagcacg gttgcatcac cacgcggtgt
     3541 gcggctaacg ccttgttgcg ggataagaat ggcgtcaggt tggacgcctt catcaatccg
     3601 tgcacgcaca aacatacccg gcaaaagcgt atgttgcggg ttagggaaga cagcacgtag
     3661 ggttatggag ccggtgcatt catcaacggt cacatcagag aattgcagcg tacctttcag
     3721 gggataggtt tgaccgtttt ccatgaccaa ctctacgttg ctggtggcgt tttccttatg
     3781 caaatttcct tgctctacgg attgcttcag cctcataaaa tcgttgctgg attgggtcac
     3841 atcaacgtag ataggatcga gctgctggac agtcgccagt tcagtcgttt gcccattagt
     3901 gacaagagcg ccttcggtca cggtcgattt gccgatacgt ccgctaattg gcgcagtcac
     3961 tttggtataa gcgagattga tgcgagcgct ttcgactgtg gcttttgcgg caatcacggc
     4021 ggcatcggcc tggcgagcat cagcaatggc ctggtcgtac tcctgctggc tgatgtattt
     4081 cgtacccacg agcggaacgt aacgttttac cgtcaaatgc gcgatggcgg cggcggcttc
     4141 acttttcgcc agttcgcctt tcgcgctgtc ataatttgcc tgataggtcg cgggatcgat
     4201 ctggtacagg gactggcctg cttgtacatc gctgccttca gtgaaattac gattcagtac
     4261 gatcccgcta acctgtgggc gaacttcggc tatacggtaa gcattggtgc ggcctggtaa
     4321 ttcagtctta acttctaacg gggccgtttt cacaatatga acggtaacct gcggttcacc
     4381 gacgtgagct ttctcttctc ccttatcgtt acaaccgacg attaaagccg cggagatcag
     4441 aataaaggag ggcaggagga aaaacctggc atgtttcgtc attactattc ctcaaaaaaa
     4501 caaaagcgcg ttatttaccc agaaaggcaa cgcgttaact cgcagaaaga aaaatacagt
     4561 ttgctatcct acaaattatc attcgtcgat gtaaggaata gttatgaata ctggcgtctc
     4621 aaggcacata aacacaaaaa aagattaata ttctactgtt ttattttgac gcgggttgaa
     4681 agaggcagaa ttaaaacctc gtaaattgaa atatatattc atgtggcgaa tgtattttag
     4741 gtaaataata tatattattt atttgacgat ttatagcaac tcaatgttac cctctcgtat
     4801 aaatacacat tagatgatag attaaccttc gctattttct caccctgtgt cgaatatatt
     4861 tatttcctga ataattaatc atggcaaaaa gaaccaaagc cgaagctctg aagacccggc
     4921 aagaactgat tgaaactgcc atcgcccagt ttgcgcaaca tggcgtaagc aagacgacgt
     4981 tcaacgacat tgccgatgcc gctaacgtta cgcgtggcgc tatctactgg cacttcgaga
     5041 acaagaccca actgtttaat gagatgtggt tgcaacagcc ttcattgcgg gagttaatcc
     5101 aggaacactt gacggctgga ttagagcatg acccgtttca acaattgcgt gaaaaattga
     5161 ttgtcggctt gcaatatatt gccaaaattc cccgccagca ggcgttgctg aaaatcttat
     5221 atcacaaatg tgaatttaat gatgggatgc tggccgaggg agtgatacgc gaaaagatgg
     5281 gctttaatcc gcagactctc cgcgaagtat tgcaggcgtg tcagcaacaa ggttgtgtag
     5341 caaataacct cgatttagat gttgtgatga ttattattga tggtgccttc agcggaattg
     5401 ttcaaaactg gttaatgaat atggcgggtt atgatcttta taaacaagca cccgcgctgg
     5461 tcgataacgt attaagaatg ttcatgccag atgaaaacat aacgaaatta attcatcaaa
     5521 cgaatgaatt aagtgtcatg taaattcaca ttctgtcctg aaaattcagt tcgtcacagt
     5581 taaccagttt tagtggataa actgagttca tattacggca tttgtctgtt tctgttgtca
     5641 gcagttcaat ccactgcatc agtaaagtat caaacagaaa aacgaagaca gcgaaaacga
     5701 cgagccacca atacttgcga atcattacct gagcctgtgc atgtttaacc aagtctggca
     5761 ggaaatatac acgaaatgaa taatctgaaa accaggctta cacttccttt actttgcaat
     5821 gagatcgggg tcaacttcgc ttaaccggct tcgttttgac aggttgccgg tctttctttt
     5881 tttcactttc gccagttctt ccgcggagta atgcgacgcg acatccagcc gtcgaagccc
     5941 cattttgatg tactcgctgt tgatctcaat accaatgaat tttcgtccgc tggcgatggc
     6001 tacggcaccg gtagtaaagc taccggcaaa cgggtcgaga acgatatcgc ctgggttgga
     6061 agaggcgaga ataatgcgtt tcagtaaggc ttccggtttt tgcgtcgggt ggttctcata
     6121 ttcatccatc aaataacgca cgcgcggaaa atcccagacg ttacccggta ctttttgatg
     6181 attgtatggc tgtggaggat ttttgcgata atcgatcaac gcgcgctgcg atccggtttt
     6241 ggcttctacc agaatagcat caccgttgaa tgtgtagttc tttgcgtctt tcaccatcat
     6301 caggatgggt tcgtacatgg agccgtagtg ttttttcgcc tgtactccag aactgtcata
     6361 tgaccagacg atgcgacttt tgatggtaaa aagcttgcgg cactggagat cgataaaggg
     6421 catgttttcc gtactgttca taatgtacat gctgccctgc tttttcagaa cgcggtggca
     6481 ctctacaatc acttcaaaca gccagtcgat aaacagatct tctttccagg cttcgatcag
     6541 accatcaaaa tttttaccga tgttatacgg tgggtcggca aagatcagat cgacgctttc
     6601 agtaggtagc tttttaagtt cggcaagggc atcaccgtga ataatggtct tagcttcatt
     6661 accaaaccgg gtcggttcac atcctgttct catggtcact ccctttgtga cacctataaa
     6721 aaaggcgctt ccccatgccg agtagcgcct ttttaatcaa gcatttagct aacctgaatt
     6781 agttcatgcc gtattttttc aattttttac gcagcgtacc acggttgatg cccatcatca
     6841 gcgcagcacg ggtctggtta ccacgggtgt attgcatcac catgtccaac aggggctgtt
     6901 ctacttcagc cagtaccagc tcatagaggt cattcacatc ctgaccattc agttgagcaa
     6961 aatagttctt cagtgcctgt ttaaccgagt cacgcagggg tttttgggtt acctgatcct
     7021 gagagttaac ggtagaaacg gtcagtacgt cagaatttac gcgttgttcg aacatagttc
     7081 tgtcagctct ttatttctgt ttacgcaaaa ttttcgaagt atgcctccaa cgcctccagc
     7141 tgttcgctgg catcctcaat ggcgttgaat gtgcgccgaa actggtcatt tggagcgtgt
     7201 tcctggagat accaggaaac gtgtttacgt gcaattcggt acccttttgc cggaccataa
     7261 aagtcatgca gttcccgaac gtgcgcgcaa agcaagcgct taacctctgc caaaggcagc
     7321 gggggcagca actccccagt gtccagataa tgctggattt cccgaaagat ccagggtctt
     7381 ccctgagctg cgcggcctat catcagggca tccgcccctg tatagtcgag cacagctctg
     7441 gctttaagcg ggtcagtaat gtcgccattc gcgataaccg gaatggaaac tttctgctta
     7501 actgcccgaa tactgtcgta ctcagcttct ccattgaaca aacaggcgcg tgtacggcca
     7561 tgaatggtca gagcctgaat gccacagtct tcagccagtt gggcaatctc ttcgcagtta
     7621 cggtgttccg gtgcccagcc ggtgcgaatc ttcagggtaa caggaacgtc cactgcattg
     7681 acgacctcgg taaggatcga tttaacgaca tccgggtact gcaagagggc tgaacctgcg
     7741 agcttgcgat tcactttttt agccgggcaa cccatattga tatcaataat ctgggcaccg
     7801 ctttccacgt taatacgtgc tgcatctgcc atttctttcg gatcgctacc agcaatttgc
     7861 acggtgcgaa taccgggttc atcaatgtgc accatccgta aacgagattt gtcgctttcc
     7921 caaacctgtg ggttagaaga catcatctcg gatactgtca atccggctcc catctcgtag
     7981 cacaacgtcc gaaaaggtct gtctgtaatg ccagccatgg gcgctgcgat caggcgattt
     8041 ctgagctgat attgtccgat gcgcatgagt taagaaatga ccatactgtg actgcaaggc
     8101 ggcgtatatt acgcattttt tgcacgagat gaaaggccaa actttgacca atcctctgag
     8161 atggatcaaa gaattgcatt taaaatgagc gtggtgagat aattactcat aaaaatcatc
     8221 atattagaaa atagtgacta aaatttacac tcaaagaaat ttgagtaagt tctcaatttt
     8281 tctttatgaa tgaaaatttt ggcacgcaaa tttgcgtaaa taaccggcaa atttacgtgc
     8341 cttttgtgag cttgctcgca cttcgccccg cgtcacccta cggcgatgcg aaggttaatt
     8401 cttacgaccg gtaatgcggc accactcttc tttttccacg accgggtcca gtgcgaagct
     8461 gtcgacataa gcttcacaaa cgctctctgc ctggcttgcc agaataccgg aaaggcccag
     8521 caaaccgcct gaaaccggca ggacgctgat taacggtgcc agttcacgta atgggcctgc
     8581 aaggatgtta gcgaccacca cgtcggcttt catttcttct ggctgatctt tcggtaagta
     8641 gagttccaga cggtcagaaa cgccattacg ttcggcgttg tcgcggctag cctgaatcgc
     8701 ctgcggatcg atatcaatac caatggcttt tgctgcaccc agtttcagcg ccgcgatagc
     8761 cagaatgccg gaaccacagc caaagtcgat gactatttta ccggttaaat cgaggctgtc
     8821 gagccactgc aggcacagag aggtagttgg atgggtaccc gtaccaaacg ccagccctgg
     8881 atctaacatc acgttgacgg cgttttcgtc cggcacatca cgccagctag ggcagatcca
     8941 cagtcgttca ccaaagcgca tcgggtggaa attatccatc cattcgcgct cccagtcttt
     9001 atcttccagt tgttcgattt tatgcgcgaa gcctgcgccg agcagcggat ggttttccag
     9061 aatcgccacc acgtcgttca tatcggtttc agcgtcgaac agaccaatca catcggtgtc
     9121 gccccacagg cgcgtttcgc ccggcagcgg ctcaaatact ggcgtatcgt gggtatcctg
     9181 aaaagtgata gaaacggcac ccgcttccat cagcgcatcg ctaagatctt cagcgttcgc
     9241 gccggtggtg ttcagtttca gttggatcca aggcatggca aaactcttta tttatcagta
     9301 gtcaaaacgg tagcttgcgg gacggatgta ccgaaacggt ttccgaccag gaaagccagc
     9361 aaacttagta gtaacgaggg cacgataggg tggaagccca ggtactgaat attcagcgtc
     9421 gcgagtacgg catacagcac gccgccaacg atcatcgcac ttagcgcgcc tttggcgttg
     9481 gcgcgttccc agtaaagacc cagcaccagc ggccacagga aaacggcttc cagcccaccg
     9541 aaggccagca aattcagcca gatgatcatt tctggcggct tccaggcggc aagcagcagc
     9601 aacgcgccga gaactaacgt aattaccgcc gacatccgct tcagacgcgt ctcgttttgc
     9661 atttgatccg gacggatatt cagatagaga tctttaatga tcgtagcgga actttgtagc
     9721 aattgggcgt taattgtcga catgatcgca gccataggtg cagccagaaa gatcccggca
     9781 gcaaacggtg gcagcacttt taccattaac gtcgggatca ccagatccgg tacggtgaga
     9841 tcggggatca ctgcacgacc taacgctccg gctaggtgca taccgaacat caggattgcg
     9901 actacaatcg taccgatgat gatcccccgg tgtaccgctt tgctgtcttt ataagagata
     9961 cagcgcaccg cagtatgcgg caggccaatc acgccaaagc acaccagtac ccagaacgac
    10021 gtcataaagg caggcgacag aatatcgtca gcgccttgtg gcgtaaccag ttgcggatcg
    10081 atggtttgca aggtctgtac tgcgttgctt aagccgccag cggcatgtac cacgccaata
    10141 agcagcacaa cggtgccaat cagcatcaca agcccttgca tggtgtcgtt cagcacgctg
    10201 gcgcgaaagc cgccaaaggc ggtatacaac gcaatgctga taccgaaaat cagcagcccg
    10261 gtttcataag gaatacccgc cgcggtttcc agcaggcgcg caccgccgat aaactgcacg
    10321 gtcattgccc caacgaacgc aaccagcaaa ctcaaactcg ccagccacac cagaagacga
    10381 ctctggtagc gggcaaacag catgtcgttc agcgtcactg cattgtagcg gcgcgcaaga
    10441 atcgcaaact tcttgccgag aataccgagt gaaagccaga ctgcaggaag ctgaatcatc
    10501 gccagcaata cccagcccag cccgtattta taagcagctc ctggcccgcc gataaacgaa
    10561 ctggcactga tataggtcgc ggtgagcgtc atcgccagca caataccgcc catagagcgg
    10621 ctgccgagga aatactcatt aaggaaggtg ccggtgctcc gtttacgcat cgcataaacc
    10681 gagataccga acaccaccac cagataggcg accagcggta gaattacttc aagctgcatc
    10741 gtcatcctcc agtgggatat cgcgatagat aaatttcacc atcgcccagc acagtccaat
    10801 aaacagtagc ggcgtcagga tgcaggccat ctcaaaccag cgcggaaagc cggtaaaacc
    10861 gggggcaacg ccaggtaagt aagcggctac taaccaaact gccagataca aaagggtcag
    10921 ccccagcgcc cagcgcgcct ctttatgggc ctgaacaaaa cgagtgtcca ttttttgtcc
    10981 ctgatgggtg aagaaagcga ggattgtacc ttatgggggt tgtcgatccc cagtaataaa
    11041 aaaggccgga aaatccggcc ttttgacgct tttgcagtct tatttttcct gaagaccgag
    11101 ttttttctcc agatagtgga tgttagtgcc accatgctgg aagttctcgt cattcatgat
    11161 gcggatctgc agatcaacgt tggttttgat accgtcgatg atcagctcct gcagcgcatt
    11221 cttcatgcgg gcaatcgcca cgtcacggtt ttcaccgtag caaatcagct taccgatcat
    11281 tgagtcatag tacggcggta cggtgtagcc cgcgtagata tgagactccc aacgtacgcc
    11341 aaaaccgcca ggtgcgtgga aacgggtgat tttgcctggg cttggcagga aggtgttcgg
    11401 atcttcggcg ttgatacgac attccaccgc atggccgcga acgtgaactt cttcttgctt
    11461 gatcgacagc ggttgaccgg cagcgatacg gagctgttct ttgatcaggt caacgccagt
    11521 gatcatttcg gtaaccgggt gttctacctg aatacgggtg ttcatttcga tgaaatagaa
    11581 ctcgccgttt tcgaacagaa actcgaaagt acctgcaccg cgatagccga tatcaacaca
    11641 cgctttagcg caacgttcgc cgatgtagcg acgcagttcc ggggtaatgc ccggtgctgg
    11701 cgcttcttcg accactttct ggtggcggcg ctgcatggag cagtcacgtt ccgccagata
    11761 gatagcgttg ccctgaccgt cagccagtac ctgaatctcg acgtggcgag gattttccag
    11821 gtatttctcc atgtaaacca tatcgttgct gaaagcagct ttcgcttccg cacgggtcat
    11881 ggagatggat tgtgccagtt cagcgtcgcc gcgcactacg cgcataccgc gaccaccgcc
    11941 gccgccggag gctttgataa tcaccggata accaatgcgt ttagcaatgg cacggttttt
    12001 atccatatcg tcgcccaacg ggccgtcaga acccggtacg caagggacgc ccgctttttt
    12061 catcgccgcg attgcggata ctttgtcgcc catcaggcga atggtttctg ctttcgggcc
    12121 gatgaagata aagccggagc gttcaacctg ctcggcaaag ttggcgttct cggagaggaa
    12181 gccgtaaccc ggatggattg ctactgcgcc ggtgatttca gcggcgctga tgattgccgg
    12241 gatgttcaga taacttttta ctgacggagc agggccaata cagaccgttt catctgccag
    12301 taatacgtgt tttagatcgc gatccgcgct ggagtgcaca gcgacagtct tgatgcccag
    12361 ttctttacag gcacgaagaa tacgcaatgc aatctcgccg cggttggcaa taacaatttt
    12421 atccagcatg ttcgcctcgt tactcgatga cgaccagcgg ctcgtcaaat tctaccggtt
    12481 gtccactttc gaccagaatt gctttcacgg taccggattt gtccgcttcg atctggttca
    12541 tcattttcat ggcttcaacg atgcacaggg tatcgcccac gttgactttc tgacccactt
    12601 cgatgaacgc ttttgcgtcc gggcttgggg tgcggtagaa agtaccaacc atcggggaac
    12661 gtacgatgtg accactgatt tccgctgctg ctggcgcttc catggaagga acggtcgccg
    12721 gagcggctgc gttagattga gctggctgct gcatcattgg tgcagcgtaa gcttgttgca
    12781 tcacagggaa acttgcggca ggagctgcac ggctaatgcg tactgactct tcgccttcag
    12841 aaatttccag ttcggagatg cctgattctt caaccagctc gatcagtttt ttaatcttac
    12901 gaatatccat gagtgggttc cgtactcttt gtttagtgtg attgtgacag gcgtttcacc
    12961 gccgtctgta aagcgtatgc cccgacagct gtatgcatag cgataaattc cagcaggccg
    13021 ggacgccggt ctattttgcc ccagagtatc aatgttagac ttgacggaca ttgtgcagcg
    13081 atctgctgct atcctccggc aaaaaacaaa atataccttc gttgcgccaa tgtcaccttt
    13141 tccgcagcgc aaaaactgcg tcagggagga cgaggtcgca cattataacg atttcgtagc
    13201 aattggcagc taaatactgg tctcatcagg gaagataatc aacagctaac atgtaaataa
    13261 ccttcaacac cgtgtaattt gcaacaagcc gcacaattca cgaaattagc gccatcatcg
    13321 acggaacttc ttataacgta aggtgaaaag ctgtaaagcc agccctgcga agatgagggg
    13381 ctgcaatgag ataatcttca cagaccacaa ataatgtagg gatgccagga tcgcgacaag
    13441 atagacgaca ttacgcagcg gttgctgggg attacccaat tttcactgca tcgtctggat
    13501 agatgtgaat gctaaagcac gcaaaatcaa ccgactgaca attctcagca ttaaataggt
    13561 tcgagtggtt aatttgtagt caaataaaat cagattattc accccatggt accagaaatc
    13621 agtaacaggt taagcgtagt gtcgcccagg caaagcacca taaacctaac aggcgacgga
    13681 ggcgtatcaa taatggctgt ttagaggagt atcaatgcgc atcaatttgt ggtcagtcga
    13741 aatcatgact ttccagtttg gatgatgttg tacaacttcc tgaccaaggt gttcgacgaa
    13801 agctgtccgg ctaaccgctt ttcccgttaa ccgattgaat aatgatgtca aaatgagttc
    13861 cctctcttat tattccttgc taaatggtta gttaaccttc accagcgtgc gaccctggat
    13921 ctggttatta atgatggcct cggcaaagtt cggtgcctct gacagagata tctcttttgc
    13981 cgcctgggta tagaatgatt ccggtaaatc ggcgaccagt cgctgccagg cttgtgcgcg
    14041 gcgttctggt ggcgtcatta ctgaatccac cccttgcaaa cggacattac gcagaataaa
    14101 tggcatgacc gtggttggca gagtaaaacc acccgccaga ccacaggccg ccacgcagcc
    14161 gccgtaattc atttgcgcca gcacttttgc cagcactttg tcgccaacag tgtcaattgc
    14221 cccagcccag acttgttttt ccagaggacg ggattcggca aactcatcac gagggagaat
    14281 acggctggca cctaaacttt tcagatattc atgggtactt tcgcgaccgg aaacggcaac
    14341 gacctgataa cccaacttat gcagcagcgc cacggcggta ctgccgacgc caccactggc
    14401 acccgtcacg acaatctccc cgtcctgcgg gcgaacaccg gcatcttcca gcgccatcac
    14461 acacagcatg gcggtaaaac cggcagtacc gataatcatt gctttacgcg cgtccagccc
    14521 ttgcggcatg gcaaccagcc agtcaccttt cactcgcgcc tgctccgcca gcccacccca
    14581 gtggttttca ccaacgcccc agccagtgag taacacctcc tgaccggcat gaaaacgcgg
    14641 atcttcgctg gtgcgtacag ttccggcaaa atcgatccca ggaatcatcg gaaaattacg
    14701 gatgattttt cccttaccgg taatcgccag cgcatcttta tagttcaggc tcgaccagtg
    14761 aacatcgacc gtgacatcgc cctccggcag gcgactttcg tccagagtct gtactgatgc
    14821 gagagttttg ccatcctgct gttctaaaag taacgcctgc ataagtggtc ctcatgtgca
    14881 tgatggattg gaaaataatt tctgaagatt atactcgcta atggaaaagc aaagccgatg
    14941 aagcgcaata aattgcgaga taaatctgat ttgctagtat gcccgcttcc tcactatcgg
    15001 agttaacaca aggatgagat taacgacgaa attttcggcc tttgttacgc tgctcaccgg
    15061 gttaacaatt tttgtgactt tgctgggctg ttcgctaagt ttctacaacg caattcagta
    15121 taagtttagt catcgcgttc aggcggtggc gacggcgatt gatacccacc ttgtgtcgaa
    15181 tgacttcagc acattaaggc cacaaattac cgaattaatg atgtcggcag atatcgttcg
    15241 tgtagacctg ctccatggtg ataaacaggt ttataccctg gccagaaatg gtagttatcg
    15301 tccggttggc tccagcgatc tgtttcgcga actgagtgtt ccgttgataa agcatccggg
    15361 gatgtcgctg cgtctggttt atcaggatcc gatgggcaac tatttccatt ctttaatgac
    15421 taccgcgccg ctcacggggg cgattggctt tatcattgtt atgctcttcc tggcggtacg
    15481 ctggttacaa cggcaacttg ccgggcaaga attgctggaa acccgggcta ctcgtatctt
    15541 aaacggtgag cgtggctcta atgtgttggg aaccatctat gaatggccgc ccagaaccag
    15601 cagtgcgcta gatacgctgc ttcgtgaaat tcagaacgca cgcgaacaac acagccgtct
    15661 tgatacgctg atccgctctt atgccgccca ggacgtgaaa accggcctca ataaccgact
    15721 ctttttcgat aatcagttag caacgttact ggaagatcag gagaaagtag gtacccacgg
    15781 gatcgtgatg atgattcgtc tgccggattt caatatgttg agcgatactt gggggcacag
    15841 ccaggttgaa gaacagttct tcactctgac taatctgctg tcgacattta tgatgcgcta
    15901 ccctggcgca ctgctggcgc gttaccaccg cagtgatttt gctgcgctgt taccgcaccg
    15961 gacgttaaaa gaggcagaga gcatcgccgg tcagttaatc aaagccgtcg ataccttgcc
    16021 gaacaataaa atgctcgatc gcgacgatat gatccacatt ggtatctgcg cctggcgtag
    16081 tggtcaggat accgagcagg taatggaaca tgcagagtct gccacgcgta atgcgggatt
    16141 gcagggcggc aatagctggg ctatttacga tgactcgttg cctgaaaaag gacgcggtaa
    16201 tgttcgctgg cgtacgctta tcgagcaaat gctcagtcgc ggcggcccgc gcctttatca
    16261 aaaaccggcg gttactcgcg aaggtcaggt tcatcatcgc gaactcatgt gccgcatctt
    16321 cgatggtaat gaagaggtta gctcggcgga gtatatgccg atggtcttgc agtttggctt
    16381 atcggaagag tatgaccgtc tgcaaatcag ccgtcttatt ccactattgc gttactggcc
    16441 agaggaaaat ctggcgattc aggttaccgt tgagtcgctg attcgcccgc gttttcagcg
    16501 ttggctgcgc gatacgttaa tgcaatgtga aaaatcacaa cgaaaacgca taattattga
    16561 acttgcagag gccgatgtag gtcaacatat cagtcgttta caacctgtta ttcgtttagt
    16621 gaatgcttta ggggtacggg tagccgtcaa ccaggctggt ttgacgctgg taagcaccag
    16681 ttggatcaaa gaacttaatg ttgagttact caagctccat ccggggctgg tcagaaacat
    16741 tgagaagcga acggagaacc agctgctggt tcaaagcctg gtggaagcct gctccgggac
    16801 cagcacccac gtttacgcca ccggcgtgcg ttcgcgaagc gagtggcaga ccctgattca
    16861 gcgcggtgtt acaggcgggc aaggggattt tttcgcgtcc tcacagccac ttgatactaa
    16921 cgtgaaaaaa tattcacaaa gatactcggt ttaacctgcc gtttaatccg ttttcacgta
    16981 gaataacgcg cgctgcgtct catgggagtg tgcttgtctg ctcgccagat tgttgcagca
    17041 catatgcaga tgaatgacct tacgcggttg caaacaggcg aggaatgctg ctgatgcatt
    17101 aagcctttct ggactcaggc agagatttgt aacaaaggaa acgaactgca ctaattttca
    17161 ccgtagcaga tgatttttgc gccttgtcgc tgctgcgtgt ggttggtaaa gtaagcggat
    17221 tttcttttcc gccccagctt tcaggattat cccttagtat gttgaaaaaa tttcgtggca
    17281 tgttttccaa tgacttgtcc attgacctgg gtactgcgaa taccctcatt tatgtaaaag
    17341 gacaaggcat cgtattgaat gagccttccg tggtggccat tcgtcaggat cgtgccggtt
    17401 caccgaaaag cgtagctgca gtaggtcatg acgcgaagca gatgctgggc cgtacgccgg
    17461 gcaatattgc tgccattcgc ccaatgaaag acggcgttat cgccgacttc ttcgtgactg
    17521 aaaaaatgct ccagcacttc atcaaacaag tgcacagcaa cagctttatg cgtccaagcc
    17581 cgcgcgttct ggtttgtgta ccggttggcg cgacccaggt tgaacgccgc gcaattcgtg
    17641 aatccgcgca gggcgctggt gcccgtgaag tcttcctgat tgaagaaccg atggctgccg
    17701 caattggtgc tggcctgccg gtttctgaag cgaccggttc tatggtggtt gatatcggtg
    17761 gtggtaccac tgaagttgct gttatctcct tgaacggcgt ggtttactcc tcttccgtgc
    17821 gcattggtgg tgatcgtttc gacgaagcta tcatcaacta tgtgcgtcgt aattacggtt
    17881 ctctgatcgg tgaagccacc gcagaacgta tcaagcacga aatcggttcg gcttacccgg
    17941 gcgatgaagt ccgtgaaatc gaagttcgtg gccgtaacct ggcagaaggt gttccacgcg
    18001 gttttaccct gaactccaac gaaatcctcg aagcactgca ggaaccgctg accggtattg
    18061 tgagcgcggt aatggttgca ctggaacagt gcccgccgga actggcttcc gacatctccg
    18121 agcgcggcat ggtgctcacc ggtggtggcg cactgctgcg taaccttgac cgtttgttaa
    18181 tggaagaaac cggcattcca gtagttgttg ctgaagaccc gctgacctgt gtggcgcgcg
    18241 gtggcggcaa agcgctggaa atgatcgaca tgcacggcgg cgacctgttc agcgaagagt
    18301 aatcggatgc aggcagggga aatgtctgtt taccctgcct ggtctgatac gagaatacgc
    18361 ataacttatg aagccaattt ttagccgtgg cccgtcgcta cagattcgcc ttattctggc
    18421 ggtgctggtg gcgctcggca ttattattgc cgacagccgc ctggggacgt tcagtcaaat
    18481 ccgtacttat atggataccg ccgtcagtcc tttctacttt gtttccaatg ctcctcgtga
    18541 attgctggat ggcgtatcgc agacgctggc ctcgcgtgac caactagaac ttgaaaaccg
    18601 ggcgttacgt caggaactgt tgctgaaaaa cagtgaactg ctgatgcttg gacaatacaa
    18661 acaggagaac gcgcgtctgc gcgagctgct gggttccccg ctgcgtcagg atgagcagaa
    18721 aatggtgact caggttatct ccacggttaa cgatccttat agcgatcaag ttgttatcga
    18781 taaaggtagc gttaatggcg tttatgaagg ccagccggtc atcagcgaca aaggtgttgt
    18841 tggtcaggtg gtggccgtcg ctaaactgac cagtcgcgtg ctgctgattt gtgatgcgac
    18901 ccacgcgctg ccaatccagg tgctgcgcaa cgatatccgc gtaattgcag ccggtaacgg
    18961 ttgtacggat gatttgcagc ttgagcatct gccagcgaat acggatattc gtgttggtga
    19021 tgtgctggtg acctccggtc tgggcggtcg tttcccggaa ggctatccgg tcgcggttgt
    19081 ctcttccgta aaactcgata cccagcgcgc ttatactgtg attcaggcgc gtccgactgc
    19141 aggactgcaa cgtttgcgtt atctgctgct gctgtggggc gcggatcgta acggcgctaa
    19201 cccgatgacg ccggaagagg tgcatcgtgt tgctaatgaa cgtctgatgc agatgatgcc
    19261 gcaggtattg ccttcgccag acgcgatggg gccaaagtta cctgaaccgg caacggggat
    19321 cgctcagccg actccgcagc aaccgacgac aggaaatgca gctactgcgc ctgctgcgcc
    19381 cacacagcct gctgctaatc gctctccaca aagggctacg ccgccgcaaa gtggtgctca
    19441 accgcctgcg cgtgcgccgg gagggcaata gtggcgagct atcgtagcca gggacgttgg
    19501 gtaatctggc tctctttcct cattgcgctg ttgctgcaaa tcatgccctg gccggataac
    19561 ctgattgttt tccggccaaa ctgggtgtta ctcatcttgt tgtattggat cctggccttg
    19621 cctcatcgcg taaatgtggg cacaggtttt gtgatgggtg ccatactgga tctgatcagc
    19681 ggctcgacgc ttggcgtacg cgtattggcg atgagcatca ttgcttacct ggtggcgctg
    19741 aaataccagc ttttccgcaa cctcgcatta tggcagcagg cgctggtcgt catgttgctt
    19801 tcgctggtgg tggatattat tgttttctgg gcagagtttt tagtgattaa cgtctctttc
    19861 agaccggaag tgttctggag tagtgtagtc aatggggtgc tctggccgtg gattttcttg
    19921 ctgatgcgta aagtccgtca gcagtttgca gtgcaataaa ggtttctatg acttctctgt
    19981 atttagcttc cggttctccg cgtcgtcagg agttacttgc gcaacttggt gtgacctttg
    20041 aacgtattgt tacgggcatt gaggagcagc gtcagccgca ggagagcgcg cagcagtatg
    20101 ttgtgcgtct ggcgcgcgag aaagcacagg cgggtgtcgc gcaaacggcg caggatctcc
    20161 cggtgctggg tgcggatact atcgttatcc tgaatggaga agtgctggag aaaccgcgcg
    20221 acgcagagca tgcggcgcag atgttgcgca aattatcggg tcagacccat caggtgatga
    20281 cggcagtggc gttggccgac agccagcaca ttctcgattg cctggtggtc accgatgtga
    20341 ctttcagaac gttaacagac gaagacatcg cgggctatgt cgccagcggt gaaccgttag
    20401 ataaagcagg tgcatacggt attcaggggc tgggtggctg ttttgtcagg aagataaatg
    20461 gcagctatca cgccgtagtc ggcttacctc tggttgaaac gtatgaatta ttaagtaatt
    20521 ttaacgcact gcgtgagaaa agggataaac atgacggctg aattgttagt aaacgtaacg
    20581 ccttcggaaa cgcgagtggc gtatattgat ggcggtattc tgcaggaaat tcatattgaa
    20641 cgtgaggcgc gacgcggaat agtaggcaat atctacaagg gtcgtgtaag tcgtgtactt
    20701 ccgggtatgc aggcggcttt tgtagatatt gggctggata aagccgcgtt tcttcatgca
    20761 tccgatatca tgccgcacac cgaatgtgtg gcgggtgaag aacaaaagca attcacggtg
    20821 cgcgacatct cggaactggt tcgtcagggg caagatctga tggtgcaggt ggtgaaagat
    20881 ccgcttggca ctaaaggtgc gcgcctgacc accgatatca cgctgccttc tcgctatctg
    20941 gtctttatgc caggggcttc tcacgttggg gtttcccaac gtattgaaag cgaatcagaa
    21001 cgtgaacgcc tgaaaaaagt ggtcgcagag tattgcgacg agcagggcgg gtttatcatc
    21061 cgtaccgcag cggaaggggt tggcgaggct gaactggcct ccgatgccgc ttatctgaaa
    21121 cgcgtctgga ccaaagttat ggagcgtaaa aagcgcccgc agacccgtta tcagctgtac
    21181 ggcgaactgg cgctggctca gcgtgttctg cgtgatttcg ccgacgccga actggaccgc
    21241 attcgcgttg actcacgcct gacttacgaa gcattacttg agttcacctc ggagtacatt
    21301 cccgagatga ccagcaagct ggagcattac accggacgcc agccgatttt cgatctcttt
    21361 gatgtcgaaa acgaaatcca gcgagcgctg gaacgcaaag tagaactgaa atccggtggt
    21421 tatctcatta tcgaccagac cgaagcgatg accaccgtgg acatcaatac cggagcgttt
    21481 gtcggtcatc gcaatctgga cgacaccatt ttcaatacca atattgaagc gacgcaggct
    21541 atcgctcgcc agttacggtt gcgtaatctg ggcgggatta tcattattga tttcatcgat
    21601 atgaataatg aagatcaccg ccgccgcgtg ctgcactcgc tggagcaggc gttgagcaaa
    21661 gaccgggtga aaaccagcgt taatggtttt tcggcgctgg ggctggtgga gatgacgcgt
    21721 aaacgcaccc gcgaaagcat tgagcacgta ctgtgtaacg aatgcccaac ctgccacggt
    21781 cgcggaacgg tgaaaaccgt ggaaacggta tgctatgaaa tcatgcgcga gattgttcgt
    21841 gtccaccatg cttacgactc cgaccgtttc ctggtctatg cttctccggc agtagccgaa
    21901 gccttgaaag gcgaagagtc acactcgctg gcggaagtgg aaattttcgt tggcaaacag
    21961 gttaaagtac aaattgaacc gctctataac caggagcagt ttgacgtcgt aatgatgtaa
    22021 acagatgctg ggccgccatc cggcaaaggg tttttgagtc acatttttag cagacaagga
    22081 gtgacgggtg aggcgattgc cggggatttt actgcttact ggagccgcgc tcgttgtgat
    22141 cgctgccctg ctggttagcg gcctgcgtat tgctttaccg catcttgacg cctggcgtcc
    22201 ggaaatcctc aacaaaatag aatccgcgac tggcatgccg gtagaagcca gtcagctctc
    22261 agccagctgg cagaattttg gcccgacgct tgaagcacac gacatccgtg cagaactaaa
    22321 agatggcggc gaattttcgg ttaaacgcgt tactctggcg ctggatgtct ggcagagcct
    22381 gttacatatg cgctggcagt ttcgcgacct cactttctgg cagctgcgct ttcgcaccaa
    22441 cactcctatc accagcggtg gtagcgatga cagtctggaa gccagtcaca tcagcgatct
    22501 gtttcttcgt caatttgacc atttcgatct ccgcgacagt gaagtcagtt tcctgacgcc
    22561 atccggtcag cgtgccgagc tggcgatccc acaactcacc tggctgaacg atccacgtcg
    22621 acaccgtgcg gaaggcctgg taagcctctc cagccttacc ggacagcacg gcgtgatgca
    22681 ggtgcgcatg gatttgcgcg atgatgaggg gttgttaagc aatggtcgcg tctggctaca
    22741 ggcggatgac atcgacctga agccgtggct cggtaaatgg atgcaggaca atattgctct
    22801 ggaaacggca cagttctccc ttgaaggctg gatgacgatc gacaaaggcg atgtaaccgg
    22861 cggtgacgtc tggctgaaac agggcggtgc cagctggttg ggcgagaagc aaacgcatac
    22921 gctgtcggtg gataatctga ccgcgcatat tacgcgtgaa aatccgggct ggcagttctc
    22981 tattcccgat acacggatca cgatggacgg caaaccctgg ccgagcggag cattgacgct
    23041 ggcctggata ccggaacagg acgttggcgg caaagacaat aaacgcagtg acgaactccg
    23101 gattcgcgcc agtaatctgg agctggcagg cctggagggc atacgcccgc tggccgcgaa
    23161 actttcacct gcactgggtg atgtttggcg ctccacacaa ccgagcggca agattaacac
    23221 tctggcgctg gatatcccgc ttcaggcggc agacaagacc cgttttcagg catcgtggag
    23281 cgatctggcc tggaagcaat ggaaattatt accgggtgcg gaacacttct ccgggacgct
    23341 ttccggcagc gttgaaaatg gtttgcttac cgcgtcgatg aagcaggcaa agatgcctta
    23401 cgaaacggta ttccgtgcgc cactggaaat cgccgacggc caggcaacta taagctggct
    23461 gaacaatgac aaaggtttcc agctggatgg gcgtaatatt gacgttaaag ccaaagccgt
    23521 ccatgcgcgc ggcggttttc gttacctgca acctgctaac gatgaaccct ggctgggtat
    23581 tctggctggc atcagtaccg atgatggttc acaagcctgg cactatttcc cggaaaactt
    23641 gatgggtaaa gacctggttg attacttaag tggcgcgatt cagggcggtg aagcggataa
    23701 cgcgacgctg gtttatggtg gcaatccgca actcttcccc tataaacaca acgaaggtca
    23761 gtttgaagtg ctggtgccgc tgcgcaacgc gaagtttgcc ttccagccgg actggcctgc
    23821 attaactaac cttgatattg aactggactt tattaacgac ggtttatgga tgaaaaccga
    23881 tggcgttaat ctgggcggcg tgcgcgcgag taatctcacc gcagtgatcc ctgactactc
    23941 gaaagaaaaa ctgctgattg acgctgacat taaaggtccg ggtaaagccg ttggccctta
    24001 ctttgatgag acaccgctga aagattctct gggtgcgacc ctgcaagaac tccagctcga
    24061 cggcgatgtg aatgctcgct tacatcttga tatcccgctg aacggcgagc tggtaaccgc
    24121 gaaaggtgaa gtgacgctgc gtaataacag tctgtttatc aaaccactcg acagcaccct
    24181 gaaaaatttg agcggtaaat tcagctttat caatggcgat ctgcaaagtg aaccactgac
    24241 agcaagctgg tttaatcagc cgttgaacgt ggatttttcc accaaagaag gggcaaaagc
    24301 ctaccaggtt gcggtgaatc tcaacggtaa ctggcaaccg gcgaaaaccg gcgttctgcc
    24361 tgaagcggtg aacgaagcat tgagtggcag cgtggcgtgg gatggtaaag tgggcattga
    24421 tctgccttat catgctggcg cgacctataa cgtagagctg aacggcgatc tgaataatgt
    24481 gagtagtcac ttaccttcac cgttagccaa acctgcgggt gaaccactag cggtaaacgt
    24541 taaggttgat ggcaatctca acagctttga attaaccgga caggctggtg cggataacca
    24601 tttcaatagc cgctggttgc ttggtcaaaa gctgacgctc gatcgtgcta tttgggcggc
    24661 agacagtaaa acgctcccgc cgttgccgga acaaagtggc gttgaactca atatgccgcc
    24721 gatgaatggt gccgagtggc tggccctgtt ccagaaaggc gctgcggaga gtgtcggtgg
    24781 tgcagcgagt ttcccacaat acataacgtt acgtacgcct atgttgtcac tgggaaatca
    24841 gcaatggaat aacctgagta ttgtttcgca accgacggca aatggcaccc tggttgaggc
    24901 gcaagggcgt gaaatcaacg ccacgctagc gatgcgtaat aacgcgccgt ggctggcgaa
    24961 tatcaaatat ctttattaca acccgagcgt ggcgaaaact cgtggtgatt caacgccgtc
    25021 atcacctttc ccgacaacgg agcgcattaa cttccgtggc tggccggacg cacaaatacg
    25081 atgcacagag tgctggttct gggggcaaaa attcggtcgc attgacagtg atatcaccat
    25141 ttctggcgat acgttaacgc tgaccaatgg actgattgat actggtttct cgcggcttac
    25201 tgccgatggt gaatgggtta ataatccggg gaatgaacgt acctcgctga aaggaaaact
    25261 gcgcgggcag aaaattgatg ccgccgcaga attttttggt gtcacgacgc ccatacgcca
    25321 gtcgtcattt aatgtggatt acgatttaca ctggcgcaaa gcaccctggc agccagatga
    25381 agcgacgttg aatggcatca ttcatactca actgggtaaa ggcgaaatta ccgaaatcaa
    25441 taccggacat gccgggcaat tgctgcgctt attgagcgta gatgccctga tgcgtaagct
    25501 gcgttttgat ttcagagaca cttttggcga agggttctat tttgactcca ttcgcagcac
    25561 cgcgtggatt aaagacgggg ttatgcacac cgacgacacg ctggtggatg gcctggaggc
    25621 ggatatcgcc atgaaagggt cggtaaatct ggtacgtcgc gacctgaata tggaagcggt
    25681 tgtcgcacca gagatttctg cgacggtggg cgtggctgcg gcttttgcgg ttaaccccat
    25741 tgttggcgcg gcagtttttg ccgctagtaa agtgctgggg ccgctgtgga gcaaagtctc
    25801 cattttgcgc tatcacattt cgggtccgct ggacgatccg caaatcaacg aagtgttgcg
    25861 ccaaccgcgt aaagaaaaag cgcaatgatt tgacgagggc gcgtaattgc cccaatctca
    25921 tatgataatc gttgccaaag gccaacgagc cagaacataa ccgtaggccg gatagggcgt
    25981 tcatgccgca tccggcagcc gtaaaaaatc ctctactgca gtaactaacg agtagcaaaa
    26041 acgatgagtc tgaacctggt aagtgaacaa ttgctagcgg cgaacagcct gaaacatcag
    26101 gacttgttcg cgatcctcgg tcaactggcc gaacgtcgcc ttgattatgg cgatctctat
    26161 tttcagtcga gctatcacga atcctgggtt ttagaagacc gcattattaa agatggttct
    26221 tacaacatcg atcagggcgt tggcgtgcgt gcaatcagcg gtgaaaaaac cggatttgct
    26281 tacgctgacc aaatcagcct gctggcgctg gaacagagtg cgcaagcggc gcgcaccatc
    26341 gtccgtgata gtggtgatgg caaagtacag acgctgggcg cggtagagca tagcccgttg
    26401 tatacctcgg tagatccgct gcaaagcatg agccgtgaag agaagctgga tatcctgcgt
    26461 cgcgtcgata aggttgctcg cgaagcggac aagcgcgtac aggaagtgac tgccagcctc
    26521 agtggtgtct atgaattaat tctggttgcg gccaccgacg gcacgctagc ggcggatgtc
    26581 cgtccgctgg tgcgtctttc cgtgagcgtt ctggtcgaag aagatggcaa acgcgaacgc
    26641 ggtgccagtg gcggcggcgg tcgttttggt tatgagttct tccttgccga tcttgacggc
    26701 gaagtccgtg cggatgcatg ggcaaaagaa gcagtgcgta tggcgctggt caatctttct
    26761 gccgttgctg caccagcggg caccatgccg gtagtacttg gcgcaggttg gccgggcgtg
    26821 ctgttgcatg aagcggtagg tcacggtctg gaaggcgact tcaaccgccg cggcacttca
    26881 gtatttagtg ggcaggtcgg ggagctggtg gcttcagaac tgtgtaccgt ggttgatgac
    26941 ggcacgatgg tcgatcgccg tggttcggtg gcgattgatg acgaaggtac gccaggccag
    27001 tacaacgtgc tgattgagaa cggcattctg aaaggctaca tgcaggataa actcaacgcg
    27061 cgtttgatgg ggatgacgcc gactggcaac ggtcgccgtg aatcctacgc ccatctgccc
    27121 atgccgcgta tgactaacac ctatatgctg ccgggtaaat cgaccccgca ggaaattatt
    27181 gaatccgttg agtacggtat ctatgcaccg aactttggtg gcggtcaggt ggatatcacc
    27241 tccggcaaat tcgttttctc cacttcagaa gcatatctga ttgaaaacgg taaagtaacg
    27301 aagccggtga aaggcgcaac gttgattggt tccggtatcg aaaccatgca gcagatttcg
    27361 atggttggca acgacctgaa actggataac ggcgtgggtg tctgcggtaa agaagggcaa
    27421 agtttgccgg ttggcgtggg ccagccaacg ctgaaagttg ataacctgac tgttggcggt
    27481 actgcgtaat aatttcatta ttttcagaag gataattaat ctttctacgt gcacgaacgg
    27541 tcccctcgct cctctggggt tagggtgagg ggaacccgtt ggcacaggtt tgtagaacgt
    27601 aacagtacaa tatgaattac ttctctttcc cgcgcccgtg catctcctga aacaatttac
    27661 cgacctcaac aaaataatcc gtcagcgagt tgatcacgac ctgtaccttc agcggcagct
    27721 tatctttttc ggtatataac gcataaaccg ggcgtggatc tgactgataa cgcggcagca
    27781 ggatctccag ctcaccgcga ttgatctcgt tgatcaccca catcagcggc acgtaggcga
    27841 tcccggcacc cgccgtcagc cagcgcacca gcgtcatcgg atcattagtc acaaatcttc
    27901 cttgtgggat caggcgagtc gagatccctt ccggtgcgat cagttcaaat tcattgtcgg
    27961 gccgcacgct gtattcaagc catgaatgac tactcaaatc ggcgggtttt tccggtatgc
    28021 cgtattgtgt gagatagctt ttcgcggcgc acaccaccat cggcatcgcg cccagacggc
    28081 gggaaaacag gctggaatcc tgcaacgcgc cgacgcggat caccacatcc agaccgtcgg
    28141 caatcaggtc gggggctgga attccggtaa ccagattgac gctcaaacct gggtattctt
    28201 tcagcatttt tgctgtcagc ccggcgagaa cattttgtgc catagttgaa gaacagccaa
    28261 tgcgcagcgt cccgatgggg gtgttattga aggcatacag ttgctcatga acatcctgca
    28321 cttcatgaag catccgacgg cagccctggt agtaaattct gccggcttcg gtcaggccaa
    28381 tgctgcgtgt gctacggttt aacagcttta cctgcaactc atcttccagt tttgacaccg
    28441 tctgactgat ggacgaaacg ctcatctgta gctgtctggc ggcggcggta aaagagccaa
    28501 attcaactac tttggcaaac accgacatgc gttttagtcg ttccattatt cactctgact
    28561 taaaagtgat ttagatcaca taatatagat aacagcataa cagttacgct aatatattaa
    28621 atatcaatct acagcaatgt tgctctcgcc cggctttcca tgcccttctc tgcggcgaca
    28681 gatgctgaaa ataataacgc ctgctctctc tttacaacca aggtcaacat gagtctgttt
    28741 cccgttatcg tggtgtttgg actgtccttc ccaccgatat tttttgaatt gcttttatca
    28801 ctggcgattt tctggctggt gcgccgggta cttgtgccaa caggtatcta cgactttgtc
    28861 tggcatccgg cgttgttcaa caccgcgctc tattgctgct tgttttattt gatatcgcga
    28921 ctgttcgttt gaggttgaag tgaaaacact aataagaaaa ttctcccgta cggccatcac
    28981 ggtcgtatta gtcattctag ccttcatcgc aatttttaat gcctgggtct attacaccga
    29041 atccccctgg acgcgtgacg cgcgctttag cgctgacgtc gttgcgatcg cgccggacgt
    29101 ttctggactc attacccagg tgaatgttca tgataaccag ctggtgaaaa aaggacagat
    29161 actgttcacc atcgaccagc cgcgctatca aaaggcgctt gaggaagcgc aagccgatgt
    29221 tgcttattat caggtactgg cacaggagaa acgccaggag gccggacgtc gtaaccgtct
    29281 cggtgtgcag gcgatgtctc gcgaagagat cgaccaggcc aacaacgtac tacaaacggt
    29341 tctgcatcag ttagcgaaag cgcaggcgac ccgcgatctg gcaaaactgg atcttgaacg
    29401 cacggtgatc cgcgcgccag cagatggctg ggtgaccaac ctcaacgtct ataccggtga
    29461 gtttattact cgaggatcaa cggcggttgc gctggtgaaa cagaactcct tctatgtact
    29521 ggcctatatg gaagaaacta agctggaagg ggggcgtccg gggtatcgtg cagagatcac
    29581 gccgcttggc agtaacaaag tgctgaaagg gactgttgat agtgttgccg caggggtcac
    29641 caacgccagc agcacgcgtg acgacaaagg gatggcgact atagactcta atcttgaatg
    29701 ggtgcgtctt gcgcaacgtg ttccggttcg tattcgtctc gacaaccagc aagagaacat
    29761 ctggcctgcg ggcaccactg ctacagtggt ggtcactggc aaacaagatc gcgacgaaag
    29821 ccaggattcg ttcttccgta aaatggccca tcgcctgcgt gagtttggtt aatcacgatg
    29881 ggtattttct ccattgctaa ccaacatatt cgctttgcgg taaaactggc gaccgccatt
    29941 gtactggcgc tgtttgttgg ctttcacttc cagctggaaa cgccacgctg ggcggtactg
    30001 acagcggcga ttgttgccgc cggtccggcc tttgctgcgg gaggtgaacc gtattctggc
    30061 gctattcgct atcgtggctt tttgcgcatc atcggcacat ttattggctg tattgccgga
    30121 ctggtgatca tcattgcgat gatccgcgca ccattattga tgattctggt gtgctgtatc
    30181 tgggccggtt tttgtacctg gatatcctcg ctggtacgaa tagaaaactc gtacgcgtgg
    30241 gggctggccg gttataccgc gctgatcatt gtgatcacca ttcagccgga accattgctt
    30301 acgccgcagt ttgccgtcga acgttgtagc gagatcgtta tcggtattgt gtgtgcgatt
    30361 atggcggatt tgctcttttc tccgcgatcg atcaaacaag aagtagatcg agagctggaa
    30421 agtttgctgg tcgcgcaata tcaattaatg caactctgta tcaagcatgg cgatggtgaa
    30481 gttgtcgata aagcctgggg cgacctggtg cgacgcacca cggcactaca aggcatgcgc
    30541 agcaacctga atatggaatc ttcccgctgg gcgcgggcca atcgacgttt aaaagcgatc
    30601 aatacgctat cgctgacgct gattacccaa tcctgcgaaa cttatcttat tcagaatacg
    30661 cgcccggaat tgatcactga tactttccgc gaattttttg acacgccggt agaaaccgcg
    30721 caggacgtcc acaagcagct caaacgcctg cggagagtta tcgcctggac cggggaacgg
    30781 gaaacgcctg tcaccattta tagctgggtc gcggcggcaa cgcgttatca gcttctcaag
    30841 cgcggcgtta tcagtaacac aaaaatcaac gccaccgaag aagagatcct gcaaggcgaa
    30901 ccggaagtaa aagtagagtc agccgaacgt catcatgcaa tggttaactt ctggcgaacc
    30961 acactttcct gcattctggg cacgcttttc tggctgtgga cgggctggac ttccggcagt
    31021 ggtgcaatgg tgatgattgc ggtcgtgacg tcactggcaa tgcgtttgcc gaatccacgc
    31081 atggtggcga tcgactttat ctatgggacg ctggccgcgc tgccgttagg gctgctctac
    31141 tttttggtga ttatccctaa tacccaacag agcatgttgc tgctgtgtat tagcctggca
    31201 gtgctgggat tcttcctcgg tatagaagta cagaaacggc gactgggctc gatgggggca
    31261 ctggccagca ccataaatat tatcgtgctg gataacccga tgactttcca tttcagtcag
    31321 tttctcgaca gcgcattagg gcaaatcgtc ggctgtgtgc tcgcgttcac cgttattttg
    31381 ctggtgcggg ataaatcgcg cgacaggact ggacgtgtac tgcttaatca gtttgttttt
    31441 gccgctgttt ccgcgatgac taccaatgtg gcacgtcgta aagagaacca cctcccggca
    31501 ctttatcagc agctgttttt gctgatgaat aagttcccag gggatttgcc gaaatttcgc
    31561 ctggcgctga cgatgattat cgcgcaccag cgcctgcgtg atgcgccgat cccggttaac
    31621 gaggatttat cggcgtttca ccgacaaatg cgccgcacag cagaccatgt gatatctgcc
    31681 cgtagcgatg ataaacgtcg tcggtacttt ggccagttgc tggaagaact ggaaatctac
    31741 caggaaaagc tacgcatctg gcaagcgcca ccgcaggtga cggaaccggt tcatcggctg
    31801 gcggggatgc tccataagta tcaacatgcg ttgaccgata gttaagtcaa aaccgacgcc
    31861 aaaagcgtcg gttttttcat ggctatactt agcgatgcac ggcagaactc gccgcgaaac
    31921 gtgacggtgg caacagatga atatttatac ctttgatttt gatgagattg agagtcagga
    31981 ggatttttat cgtgacttta gccaaacctt tggtctggct aaagataagg tacgcgatct
    32041 cgactcacta tgggatgtgt taatgaacga tgtcctgccg ctaccacttg agattgaatt
    32101 tgttcatctg ggagagaaaa cgcgtcgccg ttttggcgcg ttaatattgc tgtttgatga
    32161 ggcagaggaa gagctggaag ggcatttgcg ttttaatgtt cgtcattagc gcaaaaaaaa
    32221 gcccccgaac cgggggcaat atcgtcggac aagacgatga gggtttattt gtacagttca
    32281 gccgtagcgt gccaggtgtc accgctacga gcttcagtaa tctggtaggc cgttgcgcct
    32341 ttctcttccg cttttttgtt cagcatttca cgcatatcca ttggcgaaga cgccacacca
    32401 cttacggata cggtcccgat tgcttcacga ttttgtgctt gtgcagtatc aatggagtcg
    32461 gcagcgaatg caccgaaaga gagaacagaa agtacgctta atgcagctac agtggttttg
    32521 attttcatga tttttacctc gacataatct tttagctggg cctttgtttc gtgacccata
    32581 tcacaaaatc aagcatacac taattattga actaattaat accacgctaa ctatttcagt
    32641 tgatactaag tgttaaaaat gtgaatttta ataacaaaaa agaattaaaa acacggttat
    32701 ttattgttaa ttctagtatt ttcaattaga taatgaattt tacataatgt gcttttttta
    32761 ccgtatgtat tcattgtgtg aatgatatgt cgcggtaaaa cgcactattc gttaaattaa
    32821 tcgcagggga aagcaggggg ttgagaggaa taagcaacat tttccccgcc gccagaagcg
    32881 acggggcaga gattaaagct cctggtcgaa cagctctaaa atcgcttcgt acaggtcttt
    32941 gacggtgaaa ccgttagcag gggtggtaaa gatggtgtca tcgccagcga tggtgcccag
    33001 aataccttct gctttgccca gtgagtccag caggcgagca attaactgcg ccgcgccagg
    33061 gctggtatga atcacgacaa ctgcatcgtt gtagtcgata tccagcacca gattcttcaa
    33121 tggactggag gtggttggta cacccagttc agctggcagg cagtaaacca tttccatttt
    33181 ggcattgcgt gtacgtacag caccaaactt ggtcaacatc cgcgagactt tagactgatt
    33241 aatattgtca aagccttgct cctgcaacgc ggcgacgatt tcgccctggg agctaaattt
    33301 ctcttcttta agtaatgctt taaatgcttt aactagttct tcttgcttag ccgagcttcg
    33361 cataagtcac ccgatatggt ggttgataca acattattgt gcatacagat gaatttttat
    33421 gcaaacagtc agccctgaag aaggctgaaa taatgttatg aaagagcggg attttatcaa
    33481 atttcgttat tgagaaacat gcctgcgtca cggcatgcaa attctgttta aaagtaaatt
    33541 aattgttatc aaattgatgt tgttttggct gaacggtagg gtatattgtc accacctgtt
    33601 ggaatgttgc gctaatgcat aagcgactgt taattacgta agttaggttc ctgattacgg
    33661 caattaaatg cataaacgct aaacttgcgt gactacacat tcttgagatg tggtcattgt
    33721 aaacggcaat tttgtggatt aaggtcgcgg cagcggagca acatatctta gtttatcaat
    33781 ataataagga gtttaggatg aaagtcgcag tcctcggcgc tgctggcggt attggccagg
    33841 cgcttgcact actgttaaaa acccaactgc cttcaggttc agaactctct ctgtatgata
    33901 tcgctccagt gactcccggt gtggctgtcg atctgagcca tatccctact gctgtgaaaa
    33961 tcaaaggttt ttctggtgaa gatgcgactc cggcgctgga aggcgcagat gtcgttctta
    34021 tctctgcagg tgtagcgcgt aaaccgggta tggatcgttc cgacctgttt aacgttaacg
    34081 ccggcatcgt gaaaaacctg gtacagcaag ttgcgaaaac ctgcccgaaa gcgtgcattg
    34141 gtattatcac taacccggtt aacaccacag ttgcgattgc tgctgaagtg ctgaaaaaag
    34201 ccggtgttta tgacaaaaac aaactgttcg gcgttaccac gctggacatc attcgttcca
    34261 acacttttgt tgcggaactg aaaggcaaac agccaggcga agttgaagtg ccggttattg
    34321 gcggtcactc tggtgttacc attctgccgc tgctgtcaca ggttcctggc gttagtttta
    34381 ccgagcagga agtggctgat ctgaccaaac gcatccagaa cgcgggtact gaagtggttg
    34441 aagcgaaagc cggtggcggg tctgcaaccc tgtctatggg ccaggcagct gcacgttttg
    34501 gtctgtctct ggttcgtgca ctgcagggcg aacaaggcgt tgtcgaatgt gcctacgttg
    34561 aaggcgatgg tcagtacgcc cgtttcttct ctcaaccgct gctgctgggt aaaaacggcg
    34621 tggaagagcg taaatctatc ggtaccctga gcgcatttga acagaacgcg ctggaaggta
    34681 tgctggatac actcaagaaa gatatcgcct tgggcgaaga gttcgttaat aagtaattga
    34741 ttagcgaata ataaaaaacc ggagcacaga c
//
LOCUS       DAESHI010000037        34132 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.037, whole genome shotgun sequence.
ACCESSION   DAESHI010000037 DAESHI010000000
VERSION     DAESHI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34132
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.037"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            38..580
                     /locus_tag="KW011_001822"
     CDS             38..580
                     /locus_tag="KW011_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBH7660167.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            663..1364
                     /locus_tag="KW011_001823"
     CDS             663..1364
                     /locus_tag="KW011_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBH7660168.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1389..3988
                     /locus_tag="KW011_001824"
                     /pseudo
     CDS             1389..3988
                     /locus_tag="KW011_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3979..5049
                     /locus_tag="KW011_001825"
     CDS             3979..5049
                     /locus_tag="KW011_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBH7660169.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5061..5603
                     /locus_tag="KW011_001826"
     CDS             5061..5603
                     /locus_tag="KW011_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBH7660170.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5611..6126
                     /locus_tag="KW011_001827"
     CDS             5611..6126
                     /locus_tag="KW011_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBH7660171.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6092..6829
                     /locus_tag="KW011_001828"
     CDS             6092..6829
                     /locus_tag="KW011_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBH7660172.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6940..7950
                     /gene="pyrD"
                     /locus_tag="KW011_001829"
     CDS             6940..7950
                     /gene="pyrD"
                     /locus_tag="KW011_001829"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HBH7660173.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8124..8666
                     /gene="zapC"
                     /locus_tag="KW011_001830"
     CDS             8124..8666
                     /gene="zapC"
                     /locus_tag="KW011_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HBH7660174.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="KW011_001831"
     CDS             complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="KW011_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HBH7660175.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10016..12124
                     /gene="rlmKL"
                     /locus_tag="KW011_001832"
     CDS             10016..12124
                     /gene="rlmKL"
                     /locus_tag="KW011_001832"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HBH7660176.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNLGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12136..14043
                     /locus_tag="KW011_001833"
     CDS             12136..14043
                     /locus_tag="KW011_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH7660177.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14173..15426
                     /gene="pqiA"
                     /locus_tag="KW011_001834"
     CDS             14173..15426
                     /gene="pqiA"
                     /locus_tag="KW011_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HBH7660178.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15431..17071
                     /gene="pqiB"
                     /locus_tag="KW011_001835"
     CDS             15431..17071
                     /gene="pqiB"
                     /locus_tag="KW011_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HBH7660179.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17068..17631
                     /gene="pqiC"
                     /locus_tag="KW011_001836"
     CDS             17068..17631
                     /gene="pqiC"
                     /locus_tag="KW011_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HBH7660180.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17887..18054
                     /gene="rmf"
                     /locus_tag="KW011_001837"
     CDS             17887..18054
                     /gene="rmf"
                     /locus_tag="KW011_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HBH7660181.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="KW011_001838"
     CDS             complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="KW011_001838"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HBH7660182.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18711..20471)
                     /locus_tag="KW011_001839"
     CDS             complement(18711..20471)
                     /locus_tag="KW011_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HBH7660183.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20657..21109
                     /gene="matP"
                     /locus_tag="KW011_001840"
     CDS             20657..21109
                     /gene="matP"
                     /locus_tag="KW011_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HBH7660184.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="KW011_001841"
     CDS             complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="KW011_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HBH7660185.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22580..23089)
                     /gene="sulA"
                     /locus_tag="KW011_001842"
     CDS             complement(22580..23089)
                     /gene="sulA"
                     /locus_tag="KW011_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HBH7660186.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23308..23937
                     /gene="sxy"
                     /locus_tag="KW011_001843"
     CDS             23308..23937
                     /gene="sxy"
                     /locus_tag="KW011_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HBH7660187.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23900..26062)
                     /gene="yccS"
                     /locus_tag="KW011_001844"
     CDS             complement(23900..26062)
                     /gene="yccS"
                     /locus_tag="KW011_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HBH7660188.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26072..26518)
                     /locus_tag="KW011_001845"
     CDS             complement(26072..26518)
                     /locus_tag="KW011_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBH7660189.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26641..28695
                     /gene="helD"
                     /locus_tag="KW011_001846"
     CDS             26641..28695
                     /gene="helD"
                     /locus_tag="KW011_001846"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HBH7660190.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28727..29185)
                     /gene="mgsA"
                     /locus_tag="KW011_001847"
     CDS             complement(28727..29185)
                     /gene="mgsA"
                     /locus_tag="KW011_001847"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBH7660191.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29281..29943)
                     /locus_tag="KW011_001848"
     CDS             complement(29281..29943)
                     /locus_tag="KW011_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HBH7660192.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            30116..30529
                     /locus_tag="KW011_001849"
     CDS             30116..30529
                     /locus_tag="KW011_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBH7660193.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(30574..30891)
                     /gene="hspQ"
                     /locus_tag="KW011_001850"
     CDS             complement(30574..30891)
                     /gene="hspQ"
                     /locus_tag="KW011_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HBH7660194.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30949..32139)
                     /gene="rlmI"
                     /locus_tag="KW011_001851"
                     /pseudo
     CDS             complement(30949..32139)
                     /gene="rlmI"
                     /locus_tag="KW011_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            32234..32512
                     /gene="yccX"
                     /locus_tag="KW011_001852"
     CDS             32234..32512
                     /gene="yccX"
                     /locus_tag="KW011_001852"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBH7660195.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(32509..32838)
                     /gene="tusE"
                     /locus_tag="KW011_001853"
     CDS             complement(32509..32838)
                     /gene="tusE"
                     /locus_tag="KW011_001853"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HBH7660196.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(32929..33588)
                     /gene="yccA"
                     /locus_tag="KW011_001854"
     CDS             complement(32929..33588)
                     /gene="yccA"
                     /locus_tag="KW011_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HBH7660197.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(33795..33882)
                     /locus_tag="KW011_001855"
     tRNA            complement(33795..33882)
                     /locus_tag="KW011_001855"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33846..33848),aa:Ser,seq:tga)
BASE COUNT         8829 a         8131 c         8898 g         8274 t
ORIGIN      
        1 gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc
       61 gtttataact gtggtatgtg caacgtccag cgttatggca gctgatgata atgctatcac
      121 ggatggcaaa gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc
      181 gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg
      241 agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg atgttactgt
      301 aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac ctacagcatt
      361 tgccaaccag gctaccaccg atgcagcgac gaatgttgct ctacaaatgt atctgccaga
      421 tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag cggattctgc
      481 tgagcaaaca gtgaccttta aagttgatta tatcgctacg gggaaagcga cttctggtaa
      541 tgtgaatgcg gtaacaaatt tccatattaa ctattattaa tagaactcat taattgtttt
      601 attaattagt acccctctag tgttctggag gggatattca tattttttaa gagtgactat
      661 ttatgaaaac ttgcataaca aagggaattg tgaccgtaag tttaacggca atattactca
      721 gctgctcatc cgcatgggcg gcaggtaaag gcgggattgg acttgcagca acacgtcttg
      781 tttattcaga aggtgaagag caaatttcac tgggtgtacg taacaccagt ccggatgttc
      841 cttatcttat tcagtcatgg gtgatgaccc cagataataa aaaatcagca gattttatta
      901 ttacaccacc gctatttgtg ctgaatccgg ctaatgagaa tctgttacgc attatgtaca
      961 ttggtgcgcc gttggcgaaa gacagagaaa cccttttctt cactagcgta cgggcagtcc
     1021 cttcaacaac gaagcgggaa gagggaaata ccctgaagat tgccacacaa agcgtcatca
     1081 aacttttctg gcgaccaaaa ggtttagcgt atcccttagg cgaggctccg gcgaaactgc
     1141 gttgcacttc gtcagctgac atggttacgg tcagtaaccc aacaccttat ttcattaccc
     1201 tgacagacct gaaaataggg ggaaaggtag ttaaaaatca aatgatttcc ccctttgata
     1261 aataccaatt ctctctgcca aagggggcca aaaatagcag cgtaacgtat cgaaccatca
     1321 atgattacgg ggcggaaacg ccacaactca actgtaaatc gtaagccgtc ttcagttaag
     1381 agagcgagat gtatagaact catcgacaac acagcctgtt aagctctggt ggagtgccat
     1441 cgtttattgg tgggctggtg gtgtttgtgt cggcagcgtt caatgcacaa gctgaaacct
     1501 ggttcgatcc tgcttttttc aaagatgatc cctcaatggt ggccgatttg tctcgtttcg
     1561 aaaaaggaca aaaaataacg ccaggggttt atcgtgtcga tattgttctg aatcagacaa
     1621 ttgtagatac gcgcaacgtc aattttgttg agataacgcc agagaagggg attgccgcct
     1681 gtttgacgac tgaaagcctg gatgcaatgg gtgttaatac tgatgcgttt ccggctttta
     1741 aacaactgga caaacaagcg tgtgtgccat tggcggagat tattccggat gccagcgtaa
     1801 cgtttaatgt gaataaactc cgtctggaaa tttcagtacc gcaaatcgcc atcaaaagta
     1861 atgcccgtgg ttatgtcccc cctgaacgtt gggatgaagg gatcaacgcg ctattactgg
     1921 gatattcgtt tagcggggct aacagtattc atagcagcgc agacagtgat tctggcgaca
     1981 gctattttct gaatttaaac agtggcgtta atttaggccc atggagattg cgcaacaatt
     2041 caacatggag tcgcagtagt ggccaaaccg cagaatggaa gaatctcagc agctatttgc
     2101 agcgggcggt tattccacta aaaggcgaac tgaccgtagg tgatgattat actgcaggcg
     2161 attttttcga tagtgtcagc tttcgtggtg tgcagctggc gtcagatgac aacatgctgc
     2221 cagacagcct gaaagggttt gcgcctgtgg tgcgtggtat cgccaaaagc aatgcccaga
     2281 taacgattaa gcaaaatggt tacaccattt accaaactta tgtatcgcct ggtgcttttg
     2341 aaattagtga tctctattcc acgtcgtcga gcggtgattt gttagttgaa atcaaagaag
     2401 cggacggcag cgtcaatagc tacagcgtac cgttttccag cgtgccatta ctccagcgtc
     2461 aggggcgaat caaatacgcg gtgacactgg cgaaatacag aaccaatagt aatgaacagc
     2521 aggagagcaa atttgcctag gccacgttgc agtggggcgg accgtgggga acgacatggt
     2581 atggtggtgg acaatatgct gaatattacc gtgccgccat gtttggtctg ggatttaacc
     2641 ttggcgattt cggagcaatt tcgttcgatg cgacccaggc gaagagtacg ctggcagacc
     2701 aaagcgaaca taaaggtcag tcatatcgtt ttctgtatgc caaaacgctc aaccaattgg
     2761 gcactaattt tcaattgatg ggctatcgct attcgacgtc gggtttctac accctttccg
     2821 acaccatgta taaacatatg gatggctacg aatttaatga cggtgatgat gaagatacgc
     2881 cgatgtggtc gcgttattac aatttgtttt acaccaaacg tggcaaactg caggtcaata
     2941 tctcccagca attaggcgag tacggttcgt tttatttaag tggtagccag caaacttact
     3001 ggcataccga tcaacaggat cggctattac agtttggcta caacacgcaa attaaagatc
     3061 tctcgctggg gggttcctgg aactacagta agtcccgtgg tcaacctgat gctgatcagg
     3121 tgtttgcact aaatttttcc ctgccgctca atctgttgct ccccagaagt aatgatagct
     3181 ataccaggaa aaaaattacg cctggatgac ctctaacacc agcatcgata acgaagggca
     3241 cattacacaa aacctgggtt taacggagac actactcgat gacggtaacc tgagctacag
     3301 cgtgcaacag ggatataaca gcgaggggaa aacggctaat ggtagcgcca gcatggacta
     3361 caaaggggcg tttgcagatg cccgagtggg ctacaactac agcgataacg gcagtcaaca
     3421 acaactgaac tacgctcttt caggcagttt agttgcccat tcacagggca ttaccctggg
     3481 gcaatcgctg ggggaaacta acgtgctgat tgcagcacca ggcgcagaga atactcgtgt
     3541 ggcgaacagc accgggctga aaactgactg gcgcggatat accgttgtcc cctatgccac
     3601 ttcttatcgg gaaaatcgaa tcgcacttga tgcggcatcg ttaaaacgta acgtggatct
     3661 tgaaaatgca gtagtcaacg tggttcccac caaaggggcg ttggttctgg cggagttcaa
     3721 tgcccatgcg ggagcaaggg tattaatgaa aacatcaaag cagggtatac cgctgcgttt
     3781 tggcgcgata gcgacgctgg acggcgtaca gactaatagc ggcattattg atgatgatgg
     3841 ctcgctctat atgtccggtt tgcccgctca gggagcgata actgtacgct ggggcgaagc
     3901 tcccgatcaa atttgtcata tcagttacca gcttaccgaa caacaaatta actctgcgat
     3961 tacgcgaatg gatgccatat gcagataatc tttggagaaa aatgcgtgtt attactacga
     4021 ctattttttg ccgccgtctt aatgctatgg tgcgctcaaa ctgctgctta tagcgggcag
     4081 tgtcatacca ctcaggggaa tccgtatatt ggcgtcaatt ttggcgttaa aaccctggag
     4141 gaagaagaaa atacggctgg agtagttaaa gacaaatttt atcagtggaa cgaatcgaat
     4201 gattattatg tttcctgtga ttgcgataaa gacaatgtca gaagtggccg atgggcattc
     4261 gccgcggatt caccgttagt ctatttaggc gacaactggt acaaaattaa tgactatctt
     4321 gccgccaaag ttttattgca ggttaaaggc agttctccta ctgcggttcc tttcgaaaac
     4381 gtgggcacag gggcagatac acgatggcat atttgcgatc ccggcggtca acgtttaggt
     4441 ggccaggggg ctagcggtaa tagcggtagc ttttccctga aaatattgca gccgttcgtt
     4501 ggttcggtcg tcattcctcc tatggcgctg gcgcgattat ttgaatgcta caacataccc
     4561 gcaggtgatt cctgcacgac tacaggtaca ccggttttag tgtattacct gtctggtacg
     4621 atcaattcac ttggctcatg ttccgtcaat gccggagaaa caatcgaggt cgatctgggc
     4681 gacgcatttg cggctaactt tcgtgttgta gggcataagc ctcttggggc cagaacggca
     4741 gaacttgcaa ttccagtcag gtgtaacacg ggaaacgcgg ggttagttaa cgtcaacctg
     4801 agtctgacgg caaccacaga ccccagctat ccccaggcga ttaagacgtc acgtcctggc
     4861 gtgggcgtgg tggtgaccga tagccagaac aacattattt cccctgctgg tggaacatta
     4921 ccgctctcta ttcctgatga tgcagacagt atcgcgcgaa tgaatgtcta tccagtcagc
     4981 acgacaggtg taccaccaga aaccgggcga tttgaagcca cggcaacggt gagaataaat
     5041 tttgattaac gcgtgaacgt atgaagaaaa aaacgatatt tcagtgcgtt atcttgttct
     5101 ttagcattct taacatccat gtcgggatgg ctgggcctga acaagttagt atgcatattt
     5161 atgggaatgt ggtcgatcag ggctgtgatg tcgccaccaa aagtgcatta caaaatattc
     5221 atattggtga ttttaatatc agtgattttc aggccgcgaa taccgtaagc actgctgctg
     5281 atttgaatat cgatatcacc ggttgtgccg ctagtattac tggcgcggac gtccttttta
     5341 gcggcgaggc tgacaccctt acgccgacac tgctcaaact aactgacaca ggcggaagcg
     5401 gtggtatggc aacggggatt gccgtgcaaa ttcttgatgc gcaaagtcag caagaaatcc
     5461 cgctcaatca ggtccagcct cttacgccct taaaagccgg ggataacaca ctcaaatatc
     5521 aacttcgtta taagtccaca aaggcgggag caacgggcgg taatgcgacg gcggttctct
     5581 attttgatct ggtttaccag tgaaggagtg atgttgaaac ggataatctg gattctgttc
     5641 ttactgggat taacgggggg ctgtgagcta tttgcccatg atggcacggt caacattagc
     5701 ggatcgtttc gccgtaatac atgcgtgctg gcacaggata gcaagcaaat caatgtgcag
     5761 ttaggcgatg tctcgctgac acgttttttt catgggaatt atggcccgga gaaatctttc
     5821 atcattaatt tgcaggattg cggaacagat gtcagcacgg ttgacgtcac cttttcagga
     5881 acccccgacg gagtgcagag cgaaatgctg tccattgaaa gtggtacgga tgcagccagc
     5941 gggctggcaa ttgcgattct ggatgatgcg aagatattga ttccgcttaa ccaggcgagt
     6001 aaagactaca gcctacatag cggtaaggtg ccattaactt tttatgcgca attgcgacct
     6061 gtcaatagcg atgtgcagtc gggtaaagtg aatgccagcg cgacatttgt acttcattat
     6121 gactaatacg tggaacagat tggcgctctt gattttcgcc gttttatcgc tgctggtggc
     6181 gggtgaattg cgggcaggag tggtggtcgg cggaacgcga tttatctttc cggcagacag
     6241 agaatcgata tctattttac tgactaatac cagtcaggaa tcctggctta ttaacagtaa
     6301 aatcaaccgc ccaacgcgtt gggccggggg ggaagcgtcg acagtgccag caccattact
     6361 ggccgctccg ccacttattc tcctgaagcc cggtactaca ggcacgttgc gcttgctgag
     6421 aacggaaagc gacatcttgc ctgtggatcg cgaaacgcta tttgagttaa gcattgccag
     6481 cgtgccatcc ggcaaagttg aaaatcagag cgtaaaagtg gcgatgcgct cggtatttaa
     6541 actgttctgg cgacccgaag gtttgccggg cgacccgctg gaagcttatc aacaattacg
     6601 ctggacacgg aattcgcagg gtgtacaact cactaaccca acgccttact acattaacct
     6661 gattcaggtg agcgtaaatg gtaaagcgtt aagtaatgcg ggagttgtgc cgcctaaaag
     6721 ccagcgtcag acaagctggt gtcaggcaat cgcaccgtgt catgtcgcct ggcgggcgat
     6781 aaatgattac ggcgggttga gtgcaaagaa ggagcaaaat ctgccctgaa acaggttcgg
     6841 aaaacgtttg cgtttttttt gccgcaggtc aattcccttt tggtccgaac tcgcacataa
     6901 tacgcccccg gtttgcacac cgggaatcca ggagagttca tgtactaccc cttcgttcgt
     6961 aaagcccttt tccagctcga tccagagcgc gctcatgagt ttacttttca gcaattacgc
     7021 cgtattacag gaacgccgtt tgaagcactg gtgcggcaga aagtgcctgc gaaacctgtt
     7081 aactgcatgg gcctgacgtt taaaaatccg cttggtctgg cagccggtct tgataaagac
     7141 ggggagtgca ttgacgcgtt aggcgcgatg ggatttggat cgatcgagat cggtaccgtc
     7201 acgccacgtc cacagccagg taatgacaag ccgcgtctct ttcgtctggt agatgccgaa
     7261 ggtttgatca accgtatggg ctttaataat cttggcgttg ataacctcgt agagaacgta
     7321 aaaaaggccc attatgacgg cgtcctgggt attaacatcg gcaaaaataa agatacgcca
     7381 gtggagcagg gcaaagatga ctatctgatt tgtatggaaa aaatctatgc ctatgcggga
     7441 tatatcgcca tcaatatttc atcgccgaat accccaggat tacgcacgct gcaatatggt
     7501 gaagcgctgg atgatctctt aaccgcgatt aaaaataagc aaaatgattt gcaagcgatg
     7561 caccataaat atgtgccgat cgcagtgaag atcgcgccgg atctttctga agaagaattg
     7621 atccaggttg ccgatagttt agttcgccat aatattgatg gcgttattgc aaccaatacc
     7681 acactcgatc gttctcttgt tcagggaatg aaaaattgcg atcaaaccgg tggcttaagt
     7741 ggtcgtccgc ttcagttaaa aagcaccgaa attattcgcc gcttgtcact ggaattaaac
     7801 ggtcgcttac cgatcatcgg tgttggcggc atcgactcgg ttatcgctgc gcgtgaaaag
     7861 attgctgcgg gtgcctcact ggtgcaaatt tattctggtt ttatttttaa aggtccgccg
     7921 ctgattaaag aaatcgttac ccatatctaa ttatttcttc gaattcctct ttataagata
     7981 atcagggctt tattttcagc cctggttgtt ttatattcat cgctgttgct tatttagaca
     8041 ttttgtactt ttattgttga ggttattaag cgaagcgaca atggattgtg ttgctgcggt
     8101 tttatagggt aggggagagg cagatgcgaa ttaaaccaga cgataactgg cgttggtatt
     8161 acgatgaaga gcacgatcga atgatgctcg atttagccaa tggtatgcta tttcgctcac
     8221 gttttgcgcg caagatgttg actccagatg ccttttctcc cgcaggcttt tgcgttgacg
     8281 acgccgcgct ctatttctct tttgaagaaa agtgccgcga ttttaattta tctaaagaac
     8341 aaaaagccga actggtgctg aatgcactgg tagcaattcg ctatctcaaa ccgcaaatgc
     8401 cgaaaagctg gcattttgtt tcccatggtg aaatgtgggt gcccatgccg ggcgatgcgg
     8461 cctgcgtttg gttgagcgat actcacgagc aggtcaattt gctggtcgtt gaatctggcg
     8521 aaaatgccgc actatgcctg ctggcacaac cctgcgttgt tattgcgggt cgcgcgatgc
     8581 agttgggcga cgccattaaa atcatgaacg acaggctgaa accgcaggtt aatgttgaca
     8641 gcttcagcct cgaacaggca gtctaacgcg ccaacttaag tgcagtcttc ggtacacagc
     8701 tacagcaaag aatggtgcca tcatcgccca ttgctgattt tttcagcggc gtgacttcgc
     8761 cttctaaaag ctgaacacgg caacttccac aaatgcccgc gcggcaagaa taagggatac
     8821 gaattccctg attttctaat tgctccagca acacctgttg gttatttcca cgaaatgcct
     8881 gtccctgcca gtcaatatct acatttgcgt ccggttgttg cgtgatgttg gcggtatcat
     8941 cagcggcagc tgcgccgtaa atttttgccg gagccgttgc cagaatttcc acctcatcgc
     9001 caacgcgaat cacgccgcta ttacgggcaa ttaaattctg accaaaatcg acatcgccgt
     9061 tatcctgggc agtgcggaaa gattgcaatg tttttaatgg ttcgcctgcc ggatgttttt
     9121 gccctttttc tgggctgacg gtggtgaaaa tacagcggct acaaggttta accacatcaa
     9181 acaccacatc accaatgcga atcactttcc agcgatcttc ttcccaggct gacgcgccgg
     9241 aaaccaccag attggggcgg aattgctcca tttttacact ggccggacaa cgttgttgga
     9301 gatcacgtaa cgaggcttcg ttagcaagaa ggtaaggata gccatcagca aatgacagag
     9361 gtacagtgtt gtggcgtttc acgcgccggg tcatttgtgg ccccacccag cgtaattgca
     9421 cttcgcggga gaaaaatcca cttagccatt tgttgatcgc gtctggcgca attcgcgcgg
     9481 taaaatgtgt gccccaaact tcggttggtg cgtcttgtgt ggcgaaatca gcaaaacgaa
     9541 catatgcgct actgccatct ggtgcggtga gatgcaagcc atcatgcaca ggtgaaggag
     9601 taaaccgtac catctgggga aactggcgag cggtaataaa cgtaccgtca ggttccgtga
     9661 tcataaagat gcgatcgaag gccagaccac tgacatcagc cagagcatgt gtaagaccaa
     9721 tgccgcgcat cgatttaaca ggatgaataa aaagccgggt taatgtcgcc acggtgcggt
     9781 cctcaaatga aaataagccc tcaactttat gacatgacgc gcttattagc tataatgcgc
     9841 aacaattttc ttagcgcctg tcccattagg ctattttatt tgtcagtttg gccctgggtt
     9901 gtgctcgaaa tcctcacgta cttgtgtacg ctccggtttc tccgcgctgt ccatgtccag
     9961 actgactgca acaattacgc ctactgcggt aggctcttag agtaaaagtg acgatatgaa
    10021 ttctctgttt gccagtacgg cccgtgggct ggaagagctg ttaaaaactg aactggaaaa
    10081 cctgggggcc gttgaatgcc aggtggttca gggtggggtc catttcaagg gcgacacacg
    10141 gcttgtttac cagagcctga tgtggagccg cctggcctcg cgtattatgt tgccgctggg
    10201 cgagtgtaag gtttacagcg atttagacct ctatctcggt gttcaggcga tcaactggac
    10261 agagatgttt aatcttggcg cgaccttcgc tgtccacttc agtggtttga atgacaccat
    10321 ccgcaacagt cagtacggcg cgatgaaagt gaaagacgcg atcgtcgatg ctttcacgcg
    10381 gaaaaatctg ccgcgtccaa atgttgatcg cgatgcgccg gatatccgcg ttaacgtctg
    10441 gctgcataaa gaaaccgcca gtatcgctct tgatctgagt ggtgatggtt tacatctgcg
    10501 tggctatcgc gatcgtgctg gtattgcgcc gatcaaagaa accctggcag ccgctattgt
    10561 gatgcgaacc ggctggcagc caggaacacc gctgctcgat ccgatgtgtg gttccggtac
    10621 gttgctgatt gaagcggcga tgctagcgac cgatcgcgca ccaggcttgc accgtgggcg
    10681 ttggggcttt agcggctggg tgcagcatga tgaagctatc tggcaggaag tgaaagcgga
    10741 agcgcaaact cgtgcccgta aaggcctggc tgagtatagc tctcattttt acggttcgga
    10801 cagcgacgca cgggtgattc aacgtgcgcg cactaacgcc cgtcttgcgg ggattggtga
    10861 actgatcacc tttgaggtga aagatgtcgc gcaactgacc aatccgctgc cgaaagggcc
    10921 gtacggcaca gtgttgagca acccgccata cggggaacgt ctggacagcg aaccggcgct
    10981 gattgcgctg catagcctgc tgggtcggat catgaaaaac cagtttggtg gctggaatct
    11041 ctctttgttt agtgcctcgc cggatctgct aagctgcttg cagctgcgtg cagacaaaca
    11101 gtacaaggcg aaaaacggcc cgctggactg cgtacagaaa aattaccacg ttgccgaaag
    11161 cactccagac agcaaaccgg cgatggtagc ggaagactac gccaaccgcc tgcgtaagaa
    11221 cctcaaaaaa ttcgagaagt gggctcgcca ggaaggaatt gaatgttacc gcctgtatga
    11281 cgccgatcta ccggaatata acgttgccgt tgaccgttat gccgactggg tggtggtgca
    11341 ggagtatgcg ccgccaaaaa ctattgatgc tcacaaagcg cgtcagcgtc tgttcgatat
    11401 tatcgctgca accatttcgg tactggggat tgcgccaaac aaactggtgc tgaaaacccg
    11461 tgaacgccag aagggcaaaa accagtacca gaaactgggc gagaagggcg agtttcttga
    11521 agttaccgaa tataacgctc acttgtgggt gaacctgacg gattatctcg atactggtct
    11581 gttcctcgat caccgtatcg cccgccgtat gctcggtcag atgagcaaag gcaaagattt
    11641 cctcaacctg ttctcttata ccggtagcgc caccgtgcat gcgggattag gcggcgcgcg
    11701 cagcaccacc accgtggata tgtcgcgtac ttatctggag tgggcagaac gcaacctgcg
    11761 tctgaatggc ctgaccgggc gtgcgcatcg cctgattcag gccgattgcc tggcgtggct
    11821 gcgtgaggca aatgaacagt ttgatctgat ctttatcgat ccgccaacct tctctaactc
    11881 aaaacgaatg gaagatgcgt ttgatgttca gcgcgatcat atggcgctga tgaaagattt
    11941 gaaacgtctg ctgcgtgcag gtgggacgat catgttctcg aacaacaaac gtggcttccg
    12001 tatggatctc gacggcctgg caaaactggg actgaaagca caagaaatta cgcaaaaaac
    12061 gctctcccag gatttcgccc gtaaccgcca gatccacaac tgctggctga ttaccgcagc
    12121 ctgaaaggaa tagtaatgtc attaatcagt atgcatggcg catggctgtc gttcagcgac
    12181 gcgccgcttc tcgataacgc agaactgcat atcgaagata acgaacgtgt ttgtctggtg
    12241 ggtcgcaacg gcgcgggcaa atcgacgtta atgaaaatcc tcaaccgtga acaagggctg
    12301 gatgacggtc gcattattta cgagcaagat ttgattgtag ctcgtctgca acaggatccg
    12361 ccgcgtaacg ttgagggtag cgtttatgat ttcgttgccg aaggcattga agaacaagcg
    12421 gaatatctga aacgctatca cgatatttcg cgcctggtga tgaacgaccc gagcgagaaa
    12481 aatctcaacg aactggcgaa ggttcaggaa cagttggatc accacaacct gtggcagctg
    12541 gaaaaccgca tcaacgaagt gctggcgcaa ctggggttag atcctaatgt tgcgctgtcg
    12601 tcgctttccg gcggctggtt gcgtaaagcg gcattaggac gcgcgctggt gagtaatccg
    12661 cgcgtgctgt tgcttgatga accgacaaac cacctggata ttgaaaccat cgactggctg
    12721 gaagggttcc ttaaaacctt caatgggacg attattttca tctcccacga ccgttcgttt
    12781 atccgcaata tggcgacgcg cattgttgat ctcgatcgcg gcaagctggt gacctatcca
    12841 gggaattacg accagtacct gcttgaaaaa gaagaagccc tgcgcgtgga agaactacaa
    12901 aatgccgagt tcgatcgcaa actggcgcag gaagaggtgt ggatccgcca ggggatcaaa
    12961 gcacgccgta cccgtaatga aggccgcgta cgcgccctga aagcaatgcg tcgcgagcgt
    13021 ggtgaacgtc gcgaagtgat gggtaccgca aagatgcagg tggaagaggc cagccgctcc
    13081 ggtaagatcg ttttcgaaat ggaagacgtt tgctaccagg ttgacggtaa gcaactggtg
    13141 aaagattttt cggcccaggt tctacgtggc gacaaaattg ccctgattgg tccgaatggg
    13201 tgcggcaaaa ccacgctgct gaaactgatg ctcggtcagc ttcaagcgga cagcgggcgt
    13261 attcacgttg gcaccaaact ggaagtggct tatttcgatc agcaccgcgc ggaactggat
    13321 cccgataaaa cggtgatgga taaccttgcc gaaggtaagc aagaggtgat ggttaacggc
    13381 aagccacgcc acgtattggg ctatttgcag gactttctgt tccatccgaa acgggcgatg
    13441 acgccggtac gtgcgctttc tggcggtgag cggaaccgct tgctgctggc gcgtttgttc
    13501 ctcaaaccaa gcaacttatt gattcttgac gaaccgacca acgatcttga tgtcgaaacg
    13561 ctggaactgc tggaagaact gatcgacagc tatcagggca cggtattgct ggttagccac
    13621 gatcgtcagt ttgtcgataa caccgttaca gaatgttgga tcttcgaagg cggcggtaaa
    13681 attggtcgtt atgtcggcgg ttatcatgat gcccgtggtc agcaagagca gtatgtggcg
    13741 ctcaaacagc ctgcggtgaa aaaaaccgaa gaagccgccg cggcaaaagc agaaactgta
    13801 aaacgcagca gtagcaaact aagctataaa ttgcagcgcg aactggagca gctaccgcaa
    13861 ttgctcgaag atctggaggc gaagctggaa gccctacaga cgcaggtggc ggatgcttcc
    13921 ttcttcagtc agccacatga gcagacacaa aaagtgctcg ccgatatggc tgctgcagag
    13981 caggagctgg agcaagcctt tgaacgctgg gagtatcttg aagcgttaaa aaatggtggc
    14041 tgatcgcaaa atagtcgatt taaacagaga cagctgacaa cggtaaaatt gcctgatgaa
    14101 ctgggtttat caggcatgaa aaccaacact ttgttagtca tctgtaaaat agcgcatcat
    14161 taaggagtac caatgtgcga acatcatcat gccgcgaagc acatcctgtg ctcgcagtgt
    14221 gacatgctgg tggcgttacc gcgccttgag catggtcaga aagcggcatg tccccggtgt
    14281 ggcacaacgt taaccgtggc gtgggatgcc cctcggcagc gtccgaccgc ctatgcgttg
    14341 gctgcactgt tcatgctgtt gctgtccaac ttgtttcctt ttgtgaatat gaacgttgca
    14401 ggagttacca gtgaaattac attactggaa attcccggcg tgcttttttc tgaggactac
    14461 gccagcctcg gcaccttttt cctgttgttt gtgcaactgg ttcccgcgtt ttgtctgata
    14521 accattctgt tactggtgaa tcgcgcggaa ttaccggtcc gtttaaaaga gcaactggca
    14581 cgggtgcttt ttcaactcaa aacctgggga atggcggaga ttttcctcgc tggtgtgctg
    14641 gtcagtttcg ttaaactgat ggcttacggc agcattgggg taggcagcag ctttctcccc
    14701 tggtgtttat tttgtgtcct gcaactgcgc gcttttcagt gcgttgatcg tcgctggtta
    14761 tgggacgaca tcgccccgat gccagaactg cgccagccgc taaaaccagg cgtcacgggg
    14821 atacgtcagg ggctgcgttc gtgctcctgt tgtacggcaa tccttcctgc tgatgaaccc
    14881 gtgtgcccgc gttgtagtac caaagggtac gttcggcgta gaaacagcct gcagtggaca
    14941 ctcgcgctgc ttgtaacgtc catcatgctg tatcttccgg ctaatatttt gcccatcatg
    15001 gtgacggatt tattaggctc gaagatgccg tcgacgattc tcgctggggt cattctgtta
    15061 tggagcgaag gctcttatcc cgtcgctgcg gtgatctttc tggccagtat tatggtgcca
    15121 acgttaaaga tgatcgccat cgcgtggctg tgttgggatg ccaaagggca tggcaagcgc
    15181 gacagtgaaa gaatgcattt gatttatgaa gttgttgagt ttgtaggccg ctggtcgatg
    15241 attgacgttt tcgttatcgc ggtgctctcg gcgctggtgc gtatgggagg tttaatgagt
    15301 atttatccgg caatgggtgc attaatgttt gctttagtcg tcataatgac aatgttttct
    15361 gctatgacgt ttgacccgcg tttgtcgtgg gatcgtcaac ctgaatcaga gcatgaggag
    15421 tcctgacagt atggaatcta ataatgggga agccaaaatc cagaaagtga agaactggtc
    15481 tcccgtgtgg atatttccta tcgtcacggc gctcattggg gcctgggttc ttttttatca
    15541 ttacagccat cagggactgg aagtgaccct gatcaccgcg aatgcggaag gaattgaagg
    15601 tggcaaaacc accattaaaa gccgtagcgt tgacgtcggc gtggttgaaa gcgccacact
    15661 ggctgatgat ttgacgcacg ttgaaatcaa agtgcggctg aattctggta tggaaaaatt
    15721 gctgcataaa gacaccgtct tttgggtggt gaaaccgcag attggtcgcg aagggattag
    15781 cggcctggga acgctgctgt ctggagttta tatcgaactg cagccaggcg cgaaaggcag
    15841 caaaatggat aaatacgatt tgctggactc gccaccgttg gccccgcctg atgcgaaagg
    15901 tatccgtgtg attctcgata gcaaaaaagc cgggcagctc tcgccaggag atccggtgct
    15961 gttccgtggc tatcgggtag gttcggttga aaccagcacc ttcgatacgc aaaaacgcaa
    16021 tatcagttat caactgttca tcaatgcacc ttatgaccga ctggtgacca gcaatgttcg
    16081 cttctggaaa gatagtggca ttgcggttga tctgacgtca gcggggatgc gtgtggagat
    16141 gggctcattg acaacgctgc ttagtggcgg tgttagcttt gatgtgccgg aaggtctgga
    16201 tttagggcag ccagtggcac cgaaaacagc tttcgttttg tatgatgatc agaagagcat
    16261 tcaggattcg ttgtacaccg atcacattga ttatctgatg ttctttaaag attcggtacg
    16321 cggtctgcaa ccgggagctc cggtagaatt ccggggtatt cgcctgggta ccgtaagcaa
    16381 agtgccattc tttgcgccga atatgcgtca gacatttaac gatgattacc gtattccggt
    16441 actgattcgt atcgagccag agcggctgaa aatgcagctt ggcgaaaatg cggatgttgt
    16501 tgagcacctt ggcgaattgt tgaaacgtgg tttacgcgga tcgctgaaaa ccggaaacct
    16561 ggtcactggt gcactgtatg ttgatcttga tttctatcca aatacgcctg caataaccgg
    16621 tattcgtgaa tttaatggtt atcagattat cccgactgtt agcggcggcc tggcgcaaat
    16681 ccagcaacga ctgatggaag cgttggataa gatcaacaaa ctgccattga atccgatgat
    16741 tgaacaggca accagtacgc tttctgaaag tcagcgcaca atgaaaaacc tgcaaacgac
    16801 gctggatagc atgaacaaga tcctcgccag ccagtcgatg cagcagttgc cgacggatat
    16861 gcagtcaacg ttgcgtgaat tgaatcgcag catgcagggc ttccagcctg gctccgcagc
    16921 ctacaacaag atggtggcgg atatgcagcg ccttgatcag gtgttgcgag aactgcaacc
    16981 ggtgctgaaa acgctcaatg agaagagtaa cgcgctggta tttgaagcga aggacaaaaa
    17041 agatccagag ccgaagaggg cgaaacaatg aaaaagtggc tagtgacgat tgcagcactg
    17101 tggctggccg gatgcagctc cggcgaaatt aataaaaact attaccagtt acctgtggtg
    17161 cagagcggta cacaaagtac cgccagccag ggcaatcgtc tgttatgggt agagcaggtc
    17221 actgttcctg actatctggc ggggaatggt gtggtttatc aaaccagtga tgtgaagtat
    17281 gtgattgcca acaacaactt gtgggccagc ccgttggatc aacaattgcg caacaccctg
    17341 gttgccaacc tgagtacgca actgcccggc tgggtggttg cctcccagcc tctgggaagc
    17401 gcccaggaca cgctcaatgt taccgtaacg gagtttaacg gtcgctatga tggcaaggtc
    17461 attgtcagtg gtgagtggct gttgaaccac cagggacaac tgatcaaacg tccgttccgt
    17521 ctggaaggag tgcaaactca ggatggttac gatgagatgg ttaaagtact ggccagtgtc
    17581 tggagtcagg aagccgcttc tattgcgcaa gagataaagc gtctacctta attataaaga
    17641 tttgtaaata taaccgtctc cggtatgttg cctgaggcgg tttttttgtt tctaacgtgc
    17701 ggaaaaagtt gttcctcttt acattttttg tacaaccgac atacccgtgt agctcacaaa
    17761 tatgacagtg gcgtgaattt tgcgcattga cggcagttat gattcgcggt attgcttaac
    17821 tgtgattgca catttagtaa tcactgtttt cttttccacc agaaaccagt atgagggaaa
    17881 cgaggcatga agagacaaaa acgagatcgc ctggaacggg cacatcaacg tggttatcag
    17941 gccggcatcg ccggacgctc aaaagaaatg tgtccctatc agacgctgaa tcaaaggtca
    18001 caatggctgg gaggctggcg agaagccatg gcggacaggg tagtaatggc ctgattctgt
    18061 ctctttaaaa agaaacctcc gcattgcgga ggtttcgcct tttgatactc tgtctgatta
    18121 taatcagaag gcagaagtat cctggaacag accgactttc aggtcgctgg cggtatagat
    18181 caaacgacca tcaaccagca cttctccatc cgccaggccc ataatcagac gacggttaac
    18241 aatgcgttta aagtgaatac ggtaggtcac ttttttcgct gtcggcagta cctgaccagt
    18301 gaatttcact tcgccaacgc ccagtgcgcg gcctttacct tcgccgccca gccagccgag
    18361 gtagaaccct accagctgcc acattgcgtc caggcccagg catcccggca taaccggatc
    18421 gccaataaag tggcatccga agaaccacag atccggattg atatccagtt ctgcttcaac
    18481 ataccctttg tcgaagttac cacccgtttc ggtcattttg accacacggt ccatcatcag
    18541 catattcggt gctggcaatt gcgggccttt agcgccaaac agttcaccgc gaccagaggc
    18601 aagaaggtct tcttttgtat aggattcgcg tttatctacc atgttctctg taagccttat
    18661 tttattgaag cacgcaggat agctaacacg tgtacgctga acaagtccga tcagttcgga
    18721 ataaaccagt tcagccaacg taatggccat ggaaaacggt gacgtccttc ctgttgcgat
    18781 gcttgcgcga tacgttcctg gatggtttgc atcagcgtcg tttggccttc gccatcccac
    18841 accagattta ataataacgg cagtgcgtca gtcacatcgt ctactgccca gatggtgaat
    18901 ttgccttctt ctaccgcttt cactagttca ctgtgaagac ttaaatggcg aacgttagct
    18961 gtggggataa tgacaccttg tttcccggtt aactcacgtt gctggcaaat agcaaagaag
    19021 ccttcgattt tctcatttaa accaccgacc ggctgggcgc gaccgaactg atcgactgaa
    19081 cctgtgatag cgatactctg attcaccggc acatcggcga gggcgcttat cagggcgcag
    19141 agttcagcca tcgaggcact atctccatca acttcactgt atgactgctc aaatgtcagc
    19201 gatgctgaga aggggatctg ttgctcaagc tgtagttccg acatcaggaa cgcttgcatg
    19261 atcatcatcc ctttcgcatg gatattgccg ccaagctccg ctttgcgttc gatgtcggtg
    19321 aattcaccat cgccaatatg cacaacgcag ctaatgcgag aaggttcgcc aaaagcgcgt
    19381 ggatgacccg gaaattcaat gaccgaaagg gcgttaattt gcccgatgcg ttcgccttcg
    19441 gtttcaatca ggatttgctc ctgaaggatc tcatcctgca tacgttcagc gaggaaacct
    19501 tcgcgccatt cacgctgctg cagcattaag tttagctgct cgccggagaa ggtgtcgcca
    19561 tcacacaggg aggcgacctc tttacactgg cggaggatcc actgcgggct aagcggaagt
    19621 gtttcttgtt caccggtgta gcgtgctgct tcgcggataa gtaccggcca ggcatccgct
    19681 cccggtgcag gtaagtgatt atgtctggcg gtaaatgtca cccagcgaca ccactgggtt
    19741 actgactccg catcgacaat ctgcagagta tcttcaaatt cgctataaat agcctgctct
    19801 gaaagctctg gctccatctc ctggaaatca gccaatgatt cgcgttcgcc taccagaatg
    19861 actttcagct tcaatggcat cgaaggcaca gagacgggga gagggcgcga ctcatcaaac
    19921 gcaacccagt caaaacgctc gcggttaacg atatttttca gccgcatcca cagcagaggt
    19981 tgcgccagca gtgtacgcaa agagatgatg agaataccgc catttgcctg atgcaccaga
    20041 ccaggctgca gggtaatgtc gccattaaac tggcgcaggc agccaaagag ttgctccgct
    20101 tctacccagt cggcagcgac aacttgcgtt aaagtcgcaa aattatcatc tgcactcact
    20161 gcgtgacgta agcggatgga gtggccggaa acctcatagt gaccgcccac cagttggcct
    20221 gcatcgcttt gtagcgtacg cgcggcattg gcaataagat tcagatactc agactcttcc
    20281 ggggccttcg ccagcataaa agaggaggat gctcgcgtat gcagcaactg ctccagcgca
    20341 aattgcagcc gcggttgagt atcactgaat aaaggatcgt tttcgtcaat caaatgtggc
    20401 tgagcaaata tttcctgata gctatcggta tcaggaacca ggtcacgcca tgcaagtttc
    20461 gtaatggtca aagttgatgt tttttagtct gttgtcaaag ccgcgattat accgtaaccg
    20521 gcactacagc acacgtagaa agcaccgaca atactccagg catgggcgtt aaagctcaca
    20581 ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag agttacacgg
    20641 taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg gttggaaatg
    20701 gaagtatctg gtgaagaagc atcgcgaagg ggagttaatc acccgttaca tagaagccag
    20761 tgccgcccag gaagccgtcg atgtgttgct ctcgctggaa aatgaacccg tgctggtaaa
    20821 tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc aaaccattcg
    20881 ggcaagacga aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa aatcgatcga
    20941 tctggaattt attgtctggc aacgtctggc tggtcttgcg cagcgtcgcg gtaaaacgct
    21001 ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat acgcgaataa
    21061 aatgtcttct ctgaagcagg atctgcaggc attgctgggt aaggaataac tgacgaaagt
    21121 cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc taccagacga
    21181 gaacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg atctctacgc
    21241 gacgatccgg agccaggcag tcgatcagtg cagcacgctg tttcacgttg tcacaggtgt
    21301 tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct gccgggatac
    21361 ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga ccctggttgt
    21421 aagcgtcaga accgatgcgg tcggtgtaac ccagaacaac tacggaaccg tctttcggat
    21481 ccaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc ggtttcaggg
    21541 ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg gtctgtactt
    21601 ccggtgccgg agctggagcc ggagcaacta ctggagctgc ttcgccctga ccgaaacggt
    21661 aggaaacccc caggctcagc atgccgttgt ccggacgagt gccgatggtg tgtgcgtcac
    21721 cgatgttgtt ggtccactgg tattccagac gggtagcgat ttcaggagtg atcgcgtact
    21781 caacaccgcc agcgaagacc ggagaaacgc cggtgtcgtg gtttttacca taaacgttgg
    21841 atttagtgtc tgcacgccat accataccac ccagacgagt gtagatgtcc aggtcgtcag
    21901 tgattgggta acccagttta gcggtcagtt gaacgccctg agctttgtat gcaccgtttt
    21961 caacgctgcc tttgtacggc atacgaccta accagtcgta acccatttca aagccaacat
    22021 acgggttaac ctggtaacca ccaaaagcac cagcgcccag ttggttttca tgggtcgggc
    22081 cattgttgtt gatgaaacca gtgtcatggt actgggacca gcccagttta gcaccagtgt
    22141 accaggtgtt atctttcgga gcggcctgcg ctacggtagc gaaaccagcc agtgccactg
    22201 caatcgcgat agctgtcttt ttcatttttt gcgcctcgtt atcatccaaa atacgccatg
    22261 aatatctcca acgagataac acggttaaat ccttcaccgg gggatctgct caattaactc
    22321 taccgatatc ttcggcttat gccgagcacc cctggcgatg taaagtctac aacgtagttg
    22381 aaaacttaca agtgtgaact ccgtcaggca tatgaaaaaa aagtcttgta taaggtatgt
    22441 ttaatctttt ttgtcagcga caatttacag aagaaaatcg cggaaaccgc ttcagacaag
    22501 cctccgcaag gaaaattagt cacgactgaa agcattggct gggcgacaaa aaaagttcca
    22561 ggattaatcc taaatttact taatgataca aattagagtg aatttttagc ccggaaagtt
    22621 gtctcgtggc gtgagaggat gcgcttaccg gacgcataat aaaccccata gcgttacctt
    22681 catttgccgc atcaacaagt tcagcatgct cttcttcagt caaatcatct gccaaccaac
    22741 cgatcaccac actgtaattg cccgtgcgta aagcgcgaac cattgactcc acagtgtggc
    22801 aaggggagag ctggctaatc tgcattactt tcgttaaggg tagcccagat gcctgaaccc
    22861 attcccgact cagtttttgt tgcggtgtta accagagttg ccagcgcgat tgctgaccga
    22921 gttgctgtaa caatggcaac agtagaagtt gcgtcatcat gggctgatct tcgcgataga
    22981 caacttcact gataagcccg gctgtagtgt tttccgtaga gacacgcgca attttacttg
    23041 ctgcggatga gaacgacgaa gaacgatgtg catagcctga agtgtacata atcaatccag
    23101 cccctgtgag ttactgtatg gatgtacagt acatccagtg acaacaaaga tcaaccctat
    23161 tttcggaaag agcctcgcaa attttgtcgt tggtgacggg aaaacataaa ttaatcttgc
    23221 cccttaagaa taagttgcct attttcgtag ttaacggatc cgttaatgtg aatcattctt
    23281 ttatgttatg attttaaaag gaattttatg aaaagcctct cctataagcg gatctataaa
    23341 tcacaagaat acctggcaac gttgggcaca attgaatacc gatcattgtt tggcagttac
    23401 agcctgaccg ttgacgacac ggtgtttgcg atggtttctg atggtgagtt gtatcttcgg
    23461 gcttgtgagc aaagtgcaca gtactgtgta aaacatccgc ctgtctggct gacatataaa
    23521 aagtgtggcc gatccgttac cctcaattac tatcgggttg atgaaagtct atggcgaaat
    23581 caactgaagc tggtgcgtct gtcgaaatat tctctcgatg cagcgctgaa agagaaaagc
    23641 acgcgcaata cccgggaaag actgaaagat ttgcccaata tgtcttttca tctggaagcg
    23701 attctcgggg aggtggggat taaggatgta cgggcgttac gtatacttgg ggcaaaaatg
    23761 tgttggttgc gactgcggca gcaaaacagt ctggtgacag aaaagattct gtttatgctt
    23821 gaaggtgcca ttatcggcat tcatgaagct gcgctcccgg tggcacgccg ccaggagctt
    23881 gcagaatggg ctgactctct tacgccgaaa caggagtttc ctgcggaact tgagtaatct
    23941 ggcgttgcag acgaccaatc tcaggcagta atgcaatcaa taatccaact tgttgtacga
    24001 ccagaggttc tttgctgtct gcccgtggtt caagttgctg catccgctgt ttcaggctag
    24061 ctaatgcctc attgacgcgt tcttcatcag caggttgatg atgtaacgcg tcatcaacat
    24121 agcaaactgc gtcatcaaga aacgccagaa tttcaggatt agttaactgc tcccggtgag
    24181 caccgagggc tgagatatag ctggtaaacg tatggttaag gcacagcaac cgaaacgcgg
    24241 cttcgcgaat ttgcggggta acgttcggct cgctggacat atttgatacc accgacgcca
    24301 gctcagcatc acggttgtgt gcatcgcggc gggcaatacg atacgccaga cggttatcac
    24361 gcccctgatg gtattgctcc agtatggcat cgagataccg acagttggcc tctgtggcgc
    24421 gttcgagcat gcgcggcaga ttgcgaaact gccagtcagg ccagatgtag ctcactgccg
    24481 cccacgcaat ggcacaacca atcagcgtat cgattacgcg aggtaacgct acttcaaaac
    24541 cttcacccag taagttaaaa cacagtagca ccaaaagtgt gatgaacatc gttgcatgag
    24601 cgtattgcac gttacggaag gcaaaaaaga gcacgccggt aataaccagc agcaccagct
    24661 gcccttccag tgatggcaca aaccacagca caggaatgcc aatggcgata cctaccagcg
    24721 taccaataat ccttaacttc aggcggtggc gcgtggcgtt atagtttggc tggcagacaa
    24781 acaaacttgt cagcaagatc caatacccgt gatgcattcc ggttatctga atgatggcgt
    24841 agccgaagca caacaccagc gacattctta ccgcatgacg gaagagggcg gattccggcg
    24901 tgaagtgacg gctaagacgc agccagatat cactcaaccc gtgcgggctg tcatcagcga
    24961 gctcattttc gtcattatta tggggtagtg cctgggcctg ttctgattca attgttgcca
    25021 gttgggcatc aatggcgcgt aaattgttca gcaaaaatcc cagtgttttg agtaaatcgg
    25081 cgggtgcgcc gttatcgcgc atccgctcca gcgcagcatc aatatgcgta aaagcgcgct
    25141 caaaatgcgg atcatgttga taaggctgac gcaacaaaat acagcgtgac agttgctggc
    25201 acgcctggcc ctgcatcgac atcagccgct gaaaacggaa cagcacgtcg ctgtggcgaa
    25261 aatgttcacg caatgtttga tactgaatat gagaagagct ggcacgctcg tgaatatcct
    25321 gtgcgacaaa gtaataatgc agcgtgcgac gcgttccccg ttgaccacga tcgccacgta
    25381 agcgggtcag cagcgagagt ttcgtctgat tcaatgtcgc catcagcaga ccgttggcga
    25441 gagccaaatc gtacagcggt gcctggcttt catcttcaat atcaggatca aacatgcgcg
    25501 acttgagctc aagataacgc gccagttgtt catagcaacg cgccaggttg tcctgcagcg
    25561 ggcggaccgg gaacagcaga tgaccaataa gtgtcaggac gttgtaccag acggcaccgg
    25621 ccagcagata catcggctgc tgataccagt gctcatacag tgatgttccc aacatagtgt
    25681 aaatggcgat cagcaatgca ccgaaggcaa ttgttgcata gcgttgaccc agaccgccga
    25741 gcaaaatgaa gccgctggta gagagcgtta agccaatcgc aaatagccag ggccagggaa
    25801 acagcaattc tactgaggcc gaggcgataa aaaagcagaa cagcgtaatg atgaggttac
    25861 gcaaacgtcc cgccagtcgg tcatcgagat cggtcagcgc cgctgccacc atccccagcg
    25921 ttagcggaat cgtcagtttt acatcaccca gccaccacgg aaacgctgtg gttccacaaa
    25981 gcgcaataaa aatacgcgcg taatacagcc aggcgctgtt ccaggtatag cgtttgagca
    26041 aaggacttag cataaaggcc atattagtgg attattcaaa acgacgacgt gcattggctt
    26101 cacgcgcagc ttgcgctgtt tctaccgata ccacgcgacg accaaccggc catagtgcaa
    26161 tagcggcaat tttaaagttc gcaatgccga caggaatgcc aatgattgaa atacattgtg
    26221 cgatgcccgt tgcaatgtgc atcaggcata accaccagcc aaagaaaatc agccagaaaa
    26281 tattcaatac cgtaccgcca gtattcagca gcacattttt gccagccggg ttcagttcat
    26341 cgacatgaat agcttcattg ccataaggca ccagagatag tttagtgatc tcccagcagg
    26401 atcgtgtcag cggtaaggta aaaatcagca caatactgac cagagtcgcc aacagccagc
    26461 ccagagtggt ggcaaatccg ccaagcacaa agttcagaat gttcaaaacg gtacgcataa
    26521 aacctcgctt tactgtggtt ttcagtaatg ggcggcaatt gtaacgtttt tttgggctgg
    26581 agcacgtttt ctctgacggt tacactgata agaaataatt tcgtgtggat ctacagagtc
    26641 atggaactga aagcgacaac gcttggaaaa cgtctggcac agcaccctta cgatcgggcg
    26701 gtgatcctca atgccgggat taaagtctcc ggcgatcgcc acgaatacct tattcctttc
    26761 aatcaattac tggcgattca ctgtaagcgc ggtctggtat ggggcgagct ggaatttgta
    26821 ctgccggacg aaaaagtggt gcgtctgcac ggcaccgaat ggggcgagac gcagcgtttt
    26881 taccatcatc ttgatgctca ctggcggcgg tggagtggcg agatgagcga aattgcgtct
    26941 ggtgttttac gccagcaact ggatttgatt gccacgcgca ctggagaaaa taaatggctg
    27001 acgcgtgagc aaacctctgg cgttcagcaa caaatccgcc aggctttgtc ggcgttgccg
    27061 ttgccggtta accgactgga agaattcgat aactgccgtg aggcgtggcg taaatgtcag
    27121 gcctggttga aagatattga aagcgctcgg ttgcagcata accaggcgta taccgaagcc
    27181 atgcttaccg agtatgcgga ttttttccgc caggtcgagt cttcaccgct gaatccggcg
    27241 caggcccggg cagtcgttaa tggcgagcat tctctgttag tgctggcagg tgcaggaagc
    27301 ggaaaaacgt cggtgctggt ggcccgtgca ggctggttgc tggcgcgtgg tgaagcgtcc
    27361 cctgagcaaa ttttattgct ggcgtttggt cgcaaagccg ctgaagagat ggacgagcgg
    27421 attcgcgaac ggctacatac cgaagacatt accgcacgca cgtttcatgc gctggcgctg
    27481 catattattc agcagggcag caaaaaagtt ccgatagtca gcaaactgga aaatgatacc
    27541 gctgcccgtc atgaactctt tattgctgag tggcgcaagc aatgcagcga aaagaaagcg
    27601 caggcgaagg gctggcggca atggctgacg gaagaaatgc agtggtcagt gccagaaggt
    27661 aacttctggg atgatgaaaa attacagcgt cgccttgcct ctcgcctcga tcgctgggta
    27721 agtctgatgc ggatgcacgg tggtgcacag gcagaaatga ttgccagtgc acccgaagag
    27781 attcgcgatc tgttcagtaa acgtatcaag ttgatggccc cgttattaaa agcctggaaa
    27841 ggtgcgctga aggcagaaaa cgctgtcgat ttttcgggcc ttattcatca ggcgattgtg
    27901 attctggaga aaggtcgctt tatcagcccg tggaagcata ttctggttga tgaatttcag
    27961 gatatctcgc cgcagcgggc agcgttgtta gcggcattac gcaagcaaaa cagtcagacg
    28021 acgttgttcg ctgttggtga tgactggcag gcgatttacc gattcagcgg tgcgcaaatg
    28081 tcgctcacca ccgctttcca tgaaaacttt ggtgaaggcg aacgttgtga tttagacacg
    28141 acttaccgtt ttaacagtcg tatcggtaag gtggcaaacc ggtttattca gcagaaccca
    28201 ggccagctga aaaagccgct aaacagctta accaatggag acaaaaaagc cgtcacgtta
    28261 ttggatgaga gtcaacttga cgctttgctg gataagctct ctggttatgc caaaccggaa
    28321 gagcgcattc tgatcctggc gcgttaccat cacatgaggc ctgccagcct ggaaaaagcg
    28381 gcaacacgct ggccgaagtt gcaaatcgac tttatgacca ttcatgccag caaagggcaa
    28441 caggcggatt acgtcatcat cgttggcttg caggagggaa gtgatggttt tccggctgcg
    28501 gcgcgggagt cgattatgga agaggcgcta ctgccaccgg ttgaggattt cccggacgct
    28561 gaagaacggc ggttaatgta cgtggcgctg acccgggcac gccatcgggt atgggcactg
    28621 tttaacaaag agaatccctc tccctttgtg gaaatactga aaaatctgga tgtgccggtg
    28681 gcgagaaaac cgtaagaaac aggtggcgtt tgccacctgt gcaatattac ttcagacggt
    28741 ccgcgagata acgctgataa tcggggatta gaatatcgac cgcgtcgttg aaatgcggcg
    28801 actggattat gaagtctgcc gttgccacgt tggtggcgac cggaatgttc cataccgtcg
    28861 ccagacgcag caaggctttc acgtcaggat cgtgcggcac ggcatttagt ggatcccaga
    28921 agaaaatcaa tacatcaatt ttcccttctg agatcaatgc gccaacctgc tggtcacccc
    28981 ccattgggcc actcaacatc gcgttgacgt tcatgccggt cgcgcgggaa attaagttac
    29041 cggtagtgcc tgttgcatac agtacgtgtt gttccagtaa cggttgatgc cgttccaccc
    29101 agctcatcag catttgtttg cagtgatcgt gtgccaccag cgcaatatgt ttccgcgcag
    29161 gtaaagtgcg agtcgtcagt tccataatgt acatccgtag ttaactttcc tacagattac
    29221 tgtaagcact tatcgctgca agataaagac cgaaaaagcc tgcgcacagg cacaaaaatc
    29281 tcaggaagat ggttgttttt ccgcccactg caggaaagta tttcgcgttt gtgggtcagc
    29341 cagtttaaac caatacttca gccgttgttc tgtgagcacc tgagactgcg gtggaatagc
    29401 atccagctcg gaaacacccg aaagcagcgt actgtcatct gccatcatcg tggcaaattg
    29461 cggtagtgaa gcgcgtttcg cggatttgtt atagcgttca acctcgacct cataatcaat
    29521 cgtttttgcc gttgaggtaa tggcgagaat atccagcttt accggaatcg gcgtcgcatc
    29581 gccatccaac aattcaaggc gcggcgcggc atcaaaatgg ttagcttccc gctcattctc
    29641 caggcgaggc aggcgaaaat tgacctggtt gatgagctgg gtattaaaac tgaccaccag
    29701 cggtggggag atatacagcc gttcttcact attggaaaga tgaatcgtct tctcaacgcg
    29761 aaacactaac tgatgcggtc cattatccag ttcaatgctg tccgcaccgc gcagcagaga
    29821 gctggaaacc ttcttgccgt cgagtaccag aagatcgaca tcggttgaaa gccgcagcgt
    29881 ggtggcaaaa acggataccg gcaaacataa tgcaattaag gtggtcacga tgccggtttt
    29941 cataggtagc tcctgtcaaa agaccgcccg aataataatg tgtcaaaatt tttctttaat
    30001 catacttact aacatataga catatttccc gcttttgctc tcattcattc gtattagctg
    30061 catggttggc atgtcggctt tgtcgtacac tttgttaaac agtctggagg aaataatgaa
    30121 agaaaccgat attgcaggca ttttaacgtc cactcacacc attgcgctgg tgggggcaag
    30181 cgacaaaccc gatcgtccca gctatcgcgt gatgaaatat ctactcgacc aggggtatca
    30241 cgtcattccg gtttcgccaa aagttgccgg caaaacgctg ctgggccagc aggggtatgg
    30301 cacgctggca gacgttccgg aaaaggtcga tatggtcgat gttttccgta attcagaagc
    30361 ggcgtggggc gtggcacagg aagctattgc cattggcgcg aaaaccctct ggatgcaact
    30421 gggggtgatt aatgagcaag cggcagtact ggcacgggat gccgggttga atgtggtgat
    30481 ggatcgctgc ccggctattg agatccctcg cctgggcctg gccaaataaa aaatccccgg
    30541 aaggcaaaaa ccttccgggg attcgttcag ggatcagtta cgcagacgcg gggcctggag
    30601 ttgtttgcgg atggtctgcg ccagctcatc catggatggc tgttcaggat gttcatcctg
    30661 caattcgcta cttagctggg cttcagccag gtaggtatga accggtaggc cgttatcgtc
    30721 ctccatcact acgtgatacc acggagcggc gcgaagctcg tcatttaccg ccagctcatc
    30781 aggcgacggt tcagagagcg aataaaccgg gtcgatatcc acgaccactc cgagataacc
    30841 taacagggaa tggcggacct gctggccgat accgaatttg ctggcaatca tagtcacctc
    30901 ccgggaaatc tttacctttg atataagggt aaaattccac atttcaagtt acatgacgcg
    30961 acaggcaaac cctttcagat atagcccttc cggataggta gcgatcaccg gatgatcggc
    31021 tgcctgacgg aactgctcta taaattgtac atcacggccg gcatcaattg cggcatccgc
    31081 gatgattttc tgaaataaat cgctggtcat cagaccggaa caggagaaag tcaggagaat
    31141 accgccttca ttcagcaact gaatcgccag catgttgata tctttatagc cacgacacgc
    31201 gcccatcaac tggcttttat tctcaacaaa cttcggcggg tccatcacga taacgtcaaa
    31261 tttttcaccg cgatcgcgat aagtacgcag caatttaaag acatcatcac ggacaaactc
    31321 agccttgctc agatccagtt tgttcagctc aacgttctgc cgtgcaatat ccagcgcttc
    31381 ctaggaggta tcaacgctga caacctggct gcaaccgccc atcagtgccg atacggcgaa
    31441 accaccggta taggagaaac agttcagcac acgtttattt tcaacgtagc ggcgggtagc
    31501 caggcggctg tctcgctggt ccaggtagta gcccgttttg tgtccgtgct gaatatccac
    31561 cagcagcttc atgccgtgtt cttcaatcgg cagcagggca ggcggcaact cgccggtgac
    31621 gaggccctgg gtcagctcca tcccttcttt tttacgtacc gcaacatcgc tgcgatcgta
    31681 aatcgcacat tccgggtaca gcgtttgcag ggcactaatt aatgccgcgc gctgatattc
    31741 tgccccagca ctcagcagtt gcagcaccag aaaattaccg aaacgatcga tagtaatacc
    31801 cggcaggcca tcagattctc cggcgattaa acgatagctg tcgaggccat ctttttgcgc
    31861 cagtcagtca cgccattttt gtgcttgttg caaacggcgg gaaaaaaaag caatgtcgat
    31921 agactcagac gggtcaaacg tccagacgcg cgcccggatt tgcgaagctg gcgaataagc
    31981 gccgcgtgct aaccattttc cctgatgatc aacaatatcg atggtttcac cgaggctggc
    32041 tttaccttcc atgcgggcaa cggccccgga aaagacccac ggatggcgac gaagtaatga
    32101 tttttcgcgc cctttggcta acactaaacg tacactcata atttactttt ctgtcgatgc
    32161 aaagaaatgg gcgtcattgt ccggagtttt ccatggattt gcaacgcgtc cattaaggat
    32221 aaggagagca gcaatgtcga aagtctgcat aattgcctgg gtttacgggc gagttcaggg
    32281 cgtaggattt cgctacacca cacagtacga agcgaaaaga ctgggtttaa ccgggtacgc
    32341 caaaaatctt gacgatggca gcgtggaagt ggttgcctgc ggtgaagaag ggcaggtgga
    32401 aaaattaatg cagtggttaa agagtggcgg cccgcgttct gcgcgtgtag aacgggtgct
    32461 cagcgagccg catcatccct cgggggaatt aaccgatttt cgtatccgtt aaatacattt
    32521 taccggtttt ggcaggccag caattttggt ggcttgctta gccggacctt tcggaaacag
    32581 tcggtataaa tagcggctgt tacctttttc ttcgccaaat ttattcgcca tcgcttttac
    32641 cagcatacga atcgccggag aagtattgaa ttccagatag aaatcacgca caaaacgcac
    32701 cacttcccag tgttccggtg acagcgaaat cccttcgttc tctgcaatca ccactgccag
    32761 cggctcactc cactggctgc tttctttgag atagccttcg gtatccgttt ctatctcttt
    32821 accttcgaag atcagcataa ttattactac cttaatcaga ctggcggcag tgtaacaaaa
    32881 aacaaagccc cgcataaagc gaggctatga aagtgttagc gggtgagatt aatcgcggct
    32941 ggcgaagccc agaatgctca gcaggctgac gaagatgttg tacagcgaaa catacaggct
    33001 aaccgtggca cgaatatagt tcgtttcacc gccatgaacg atgttgctgg tttcaaacaa
    33061 aatagcgcca gaggagatca gaatgaagac cgcgctgatc gccagatgca gagcaggcag
    33121 ctgcaggaag atattagcaa ccataccaat cagcaccacc acaatacccg ccatcagcat
    33181 accgccgagg aacgacatat ctttgcgggt ggtcagcaca tatgcagagc agcagaagaa
    33241 cactaacgcc gttccgccca gtgccatagc gattacgtca cccattccgg cagacagata
    33301 ggtgttcaga ataggtccga ggatataacc cagaaaaccg gtaaaggcga atgcggagat
    33361 aatcccggtc ggcttattcg ccgttttata ggtcaggaac atcaaaccat acatacccac
    33421 cagcgtcaga atcagacccg gagatggcag catcagcacc gtactggcag ttgcggtaat
    33481 cgccgaaaag gccagcgtca ggctcagcag aaaataggta ttacgcagca ccttatgggt
    33541 gctaagcagt gatgtacggt catgtgaaga actaacaata cgatccattg agtcactctc
    33601 tatgacagat gtaattaatt aagcagcata atgataatgc gtaagggcac ccagaagttt
    33661 tacccatctt tacgcatttg atctggaaca ggtttaacag cggattatca ggtcattaag
    33721 caaatataac gccctgagaa tttcgacagg caaaagaaaa aggggttagc atttagctaa
    33781 ccccttatct tatttggcgg aagcgcagag attcgaactc tggaaccctt tcgggtcgcc
    33841 ggttttcaag accggtgcct tcaaccgctc ggccacactt ccggaatgac gcgcactata
    33901 aacatcccga tgcggcgtgt aaacccctaa tttgtttgtt tgcctgaaaa acagccaaaa
    33961 gtgcattgat agcgtgaaat aacagcagat tgatcatttc atcaccatga attccttctc
    34021 ttttactcgt ttagcaaccg gctaaacatc cccaccgccc ggcaaaaaga aaaataggtc
    34081 catttttatc gctaaaagat aaatccacac agtttgtatt gttttgtgca aa
//
LOCUS       DAESHI010000038        32838 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.038, whole genome shotgun sequence.
ACCESSION   DAESHI010000038 DAESHI010000000
VERSION     DAESHI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32838
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.038"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            267..1925
                     /locus_tag="KW011_001856"
     CDS             267..1925
                     /locus_tag="KW011_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HBH7660198.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1922..2878)
                     /locus_tag="KW011_001857"
     CDS             complement(1922..2878)
                     /locus_tag="KW011_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HBH7660199.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3029..4144
                     /gene="macA"
                     /locus_tag="KW011_001858"
     CDS             3029..4144
                     /gene="macA"
                     /locus_tag="KW011_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HBH7660200.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4141..6087
                     /gene="macB"
                     /locus_tag="KW011_001859"
     CDS             4141..6087
                     /gene="macB"
                     /locus_tag="KW011_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HBH7660201.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="KW011_001860"
     CDS             complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="KW011_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HBH7660202.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6707..7027
                     /gene="clpS"
                     /locus_tag="KW011_001861"
     CDS             6707..7027
                     /gene="clpS"
                     /locus_tag="KW011_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HBH7660203.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7058..9334
                     /gene="clpA"
                     /locus_tag="KW011_001862"
     CDS             7058..9334
                     /gene="clpA"
                     /locus_tag="KW011_001862"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HBH7660204.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9740..9827)
                     /locus_tag="KW011_001863"
     tRNA            complement(9740..9827)
                     /locus_tag="KW011_001863"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9791..9793),aa:Ser,seq:gga)
     gene            complement(10081..10299)
                     /gene="infA"
                     /locus_tag="KW011_001864"
     CDS             complement(10081..10299)
                     /gene="infA"
                     /locus_tag="KW011_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBH7660205.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10584..11288)
                     /gene="aat"
                     /locus_tag="KW011_001865"
     CDS             complement(10584..11288)
                     /gene="aat"
                     /locus_tag="KW011_001865"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HBH7660206.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGQLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(11330..13051)
                     /gene="cydC"
                     /locus_tag="KW011_001866"
     CDS             complement(11330..13051)
                     /gene="cydC"
                     /locus_tag="KW011_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HBH7660207.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13052..14818)
                     /gene="cydD"
                     /locus_tag="KW011_001867"
     CDS             complement(13052..14818)
                     /gene="cydD"
                     /locus_tag="KW011_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HBH7660208.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14941..15906)
                     /gene="trxB"
                     /locus_tag="KW011_001868"
     CDS             complement(14941..15906)
                     /gene="trxB"
                     /locus_tag="KW011_001868"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBH7660209.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16451..16945
                     /gene="lrp"
                     /locus_tag="KW011_001869"
     CDS             16451..16945
                     /gene="lrp"
                     /locus_tag="KW011_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HBH7660210.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17080..21147
                     /gene="ftsK"
                     /locus_tag="KW011_001870"
     CDS             17080..21147
                     /gene="ftsK"
                     /locus_tag="KW011_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBH7660211.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21302..21913
                     /gene="lolA"
                     /locus_tag="KW011_001871"
     CDS             21302..21913
                     /gene="lolA"
                     /locus_tag="KW011_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HBH7660212.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21924..23267
                     /gene="rarA"
                     /locus_tag="KW011_001872"
                     /gene_synonym="mgsA"
     CDS             21924..23267
                     /gene="rarA"
                     /locus_tag="KW011_001872"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HBH7660213.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23358..24650
                     /gene="serS"
                     /locus_tag="KW011_001873"
     CDS             23358..24650
                     /gene="serS"
                     /locus_tag="KW011_001873"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBH7660214.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24889..27333
                     /gene="dmsA"
                     /locus_tag="KW011_001874"
     CDS             24889..27333
                     /gene="dmsA"
                     /locus_tag="KW011_001874"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HBH7660215.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27344..27961
                     /gene="dmsB"
                     /locus_tag="KW011_001875"
     CDS             27344..27961
                     /gene="dmsB"
                     /locus_tag="KW011_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBH7660216.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27963..28826
                     /gene="dmsC"
                     /locus_tag="KW011_001876"
     CDS             27963..28826
                     /gene="dmsC"
                     /locus_tag="KW011_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HBH7660217.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(28862..29488)
                     /locus_tag="KW011_001877"
     CDS             complement(28862..29488)
                     /locus_tag="KW011_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBH7660218.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29803..30951
                     /locus_tag="KW011_001878"
     CDS             29803..30951
                     /locus_tag="KW011_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH7660219.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            31161..32591
                     /locus_tag="KW011_001879"
     CDS             31161..32591
                     /locus_tag="KW011_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH7660220.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(32592..>32838)
                     /locus_tag="KW011_001880"
     CDS             complement(32592..>32838)
                     /locus_tag="KW011_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH7660221.1"
                     /translation="DVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERP
                     GFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT         8057 a         8032 c         8814 g         7935 t
ORIGIN      
        1 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc
       61 tttaggcgta ataaaaaata atatatcatg ctaattattt gattttgttg tttttgcaga
      121 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca tttgccatgc
      181 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
      241 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
      301 gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt ggggagaacg
      361 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
      421 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
      481 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
      541 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
      601 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
      661 ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgc catctggtgc
      721 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
      781 cggtgccaaa tgtccctaat atggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
      841 agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga ctttccgcaa
      901 tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg cgatatcgtt
      961 taatgcggcg gcgagccagc aatgagcaac gaagctggcg ctatctggat atcatcaacc
     1021 ggatgattga ccgacctggt gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
     1081 ctttgttgca ggcaaaaggc acattgcgac tggataaaga cgcccgtcca ttgttgctga
     1141 tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg catctgttga
     1201 atcttctgcc attgcagcgc attgtcacca ccaactcggg tgagttgctt tcgttaacgc
     1261 cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg
     1321 ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt cgttttaacc
     1381 gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg gaaacctggg
     1441 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
     1501 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
     1561 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
     1621 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
     1681 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
     1741 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
     1801 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
     1861 taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
     1921 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
     1981 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
     2041 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc cagaaagcgt
     2101 tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
     2161 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
     2221 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
     2281 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
     2341 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgaa
     2401 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
     2461 actgctcgcc gtttttgcct tccagcttcg ccagttgcag cccgggggta ttcaaatata
     2521 agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa cgtaacgcct
     2581 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
     2641 gtcgcggttg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
     2701 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
     2761 ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
     2821 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
     2881 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
     2941 tgtgattact aacgtaaatt ttatggtttt gttgatattt cgttgaagtt aatgacccgg
     3001 attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
     3061 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
     3121 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
     3181 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg ggcgcgcagg tcagcggtca
     3241 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
     3301 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
     3361 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
     3421 tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca ccgccgcgac
     3481 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
     3541 ggcttctctc gatacggcta aaaccaatct cgattacact cgcatcgttg ccccgatggc
     3601 cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg cacaacaagc
     3661 accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc aggtttctga
     3721 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtac ttggcgatcc
     3781 actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa aggttaacga
     3841 cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc tgcggctgga
     3901 tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga cgatccctct
     3961 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
     4021 cgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
     4081 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
     4141 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg taatgagcag
     4201 gttgaggtgc tgaagggcat cagcctcgat atttatgcgg gtgagatggt cgcgattgtt
     4261 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
     4321 accagcggca cctatcgcgt agccggtcag gatgttgcca cgctggacgc cgatgcgctg
     4381 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
     4441 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ctggtcttga gcggaaacag
     4501 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
     4561 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
     4621 aacggcggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag ccattctggc
     4681 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
     4741 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
     4801 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
     4861 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
     4921 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
     4981 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
     5041 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
     5101 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
     5161 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
     5221 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
     5281 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
     5341 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
     5401 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
     5461 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
     5521 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
     5581 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
     5641 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
     5701 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggtgg tattggtgta
     5761 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
     5821 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
     5881 ctggtcggtg gcgcgttggg aataactctg tcactgttaa ttgctttcac cttgcagctt
     5941 ttcttacccg gctgggagat tggtttttca ccgttggcac tgctgctggc gtttctctgc
     6001 tcgacggcca ccgggatttt atttggatgg ttacccgcac gaaatgcggc acgactggat
     6061 ccagtagatg ctctggcacg agagtaattt ttgagataaa aatgccagcc gatcgggctg
     6121 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
     6181 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
     6241 aacggattgt ccagctttta gcgttctata accatccatc tgaatggtgg aataatgagc
     6301 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg cattgttgaa
     6361 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
     6421 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
     6481 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
     6541 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
     6601 atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc tcttgtcagc
     6661 ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa
     6721 cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc
     6781 atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt ttgttattga
     6841 cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga tgctcgctgt
     6901 tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt
     6961 ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa
     7021 agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag aactggaact
     7081 cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta tgaccgtcga
     7141 gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc
     7201 tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa ccacacccgt
     7261 tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc agcgtgtact
     7321 gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg gtgcaaacgt
     7381 tctggtcgct atctttagcg aacaggagtc gcaggcggca tatctgttgc gtaaacatga
     7441 agtcagccgt ctcgatgtgg tgaattttat ctctcatggc acgcgtaaag acgagccgac
     7501 acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg gggaggaacg
     7561 tatggagaat ttcacgacga acctgaatca gcttgcgcgc gtgggcggaa tcgacccact
     7621 gattggtcgt gagaaggagc tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa
     7681 caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcgg aaggtcttgc
     7741 ctggcgaatt gttcagggcg atgtgccgga agtgatggct gactgtacga tttactctct
     7801 cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa aacgttttaa
     7861 agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg atgagatcca
     7921 caccattatc ggtgcggggg cagcgtctgg tggtcaggtt gatgcggcta acctaatcaa
     7981 accgttgctc tccagcggta aaattcgtgt aattggttcg acaacctatc aggagttcag
     8041 caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg atattactga
     8101 accgtcaatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt atgaagcgca
     8161 ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg cggtgaaata
     8221 catcaatgat cgtcatctgc cggataaagc gattgatgtt atcgacgaag cgggcgctcg
     8281 cgcacgcctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc
     8341 cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg accgcgatac
     8401 cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggtcaggata aagccattga
     8461 ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac ataaaccggt
     8521 cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa acagaggtga cggtacagct
     8581 ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg tccgagtata tggaacgcca
     8641 taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc agggcggttt
     8701 gctgaccgat gcggtaatca agcatccaca tgcggtgctg ctgctggacg aaatcgagaa
     8761 agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta cgctgaccga
     8821 taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca ccaacgccgg
     8881 ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca gcaccgatgc
     8941 gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg acaacattat
     9001 ctggtttgat catctgtcaa ccgacgtgat ccatcaggtg gtggataaat tcatcgtcga
     9061 gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa
     9121 ctggctggcc gagaaaggtt acgaccgggc aatgggcgct cgtccgatgg cgcgtgtcat
     9181 ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc tggtggacgg
     9241 cggtcaggtc accgtcgcgc tggataaaga gaaaaatgag ctgacttacg gattccagag
     9301 tgcacaaaag cacaaggcgg aagcagcgca ttaatctaat tgttaggtag gttggtgaag
     9361 tccgtaatct cgaaagaggt tacggacttt ttgtttatgg ggtggaacgg tgaaaaccct
     9421 atttttggag gtgaaggtaa tttgttgata attagtgctg ctggaaaagg taaggataaa
     9481 aaagggtgac agcaggaaaa tgagatggtt ttgctttatt aacaacggac taaacgtgta
     9541 gtatttgagt tcactgccgt acaggcagct tagaaatcga cggggtgcgg taaaaccttt
     9601 gcgaacgcgt tcactgccgt acaggcagct tagaaattca caggtaacat actccacccg
     9661 cccaccatgt tcactgccgt acagaaagat aaaatgcgaa aaaaaagctc gcactttcgt
     9721 acgagctctt ctttaaatat ggcggtgagg gggggattcg aacccccgat acgttgccgt
     9781 atacacactt tccaggcgtg ctccttcagc cactcggaca cctcaccaaa ttgttttgct
     9841 accaaacctc atgggtggca acggggcgct actataggga gttggagtaa aacggtcaag
     9901 aagaatttta atgataatta ttgtttgctc atactgtaaa caagttgtgc agtatatcta
     9961 catcgagaca agttacggac ttatacttcc aaagtacttc atacatatca caaaataaaa
    10021 aggccggtta aaccgacctt ttactcgttc tttctcttcg cccatcaggc ggtaaaacaa
    10081 tcagcgacta cggaagacaa tgcggccttt gctcaggtcg tacggggtca gttcaacagt
    10141 cactttgtcg cccgtcagga tgcggatgta gtttttgcgc attttaccgg agatgtgtgc
    10201 agtaaccacg tgaccgtttt ctaactctac gcggaacatg gtattaggca acgtttcaag
    10261 aacggtacct tgcatttcaa tattgtcttc tttggccatc taatcctctg gggtatcact
    10321 accgtaattt gaaccggcaa gataatgccg aagttctgta aataagtaaa gatttgcgcg
    10381 ctaaatcgca acaaacaggt tcggcacatt actccgaaaa cacacggcta agccgcacca
    10441 aaagcgcaac gtataaggga gcggtgagat aaacgatggg cgttacctga cgcgaaaaat
    10501 tccttatcgg cagcggggta atgagcataa ccaactctgc gaccgcaatt ataacactct
    10561 ggggagaaat gtgccgaaaa cattcatttt tgtggtgaaa acaagcatcg tggcacccag
    10621 aaattattcg gcaatcgtcc gaggcgcatt tgattgagat aattcagata atcccgacgg
    10681 ggaatttcgc aggcaccaag cgatgctgtg tgatcgttaa ggacctggca gtcgataagc
    10741 ttaccgccat gactgataaa ttcctcacag aataccagaa gcgccgtttt agacgcattt
    10801 tccatccggc tgaacatgga ctcgccacaa aatagcgttc cctgggccac gccgtacata
    10861 ccgccgacaa gctcatcttc atgccagact tcaatggagt gggcatgacc gagttcgtga
    10921 aggcgatggt aggcttcgac cacgccacgc gtgatccagg ttccttcttc gcgatcgctg
    10981 gcacaacctt caatgacctg accaaaagcg taattcatcg tgacacgata gggcgagcgt
    11041 ttatgaaatc gcttcatact acggctgata tgcagtgatt caggccaaag caccgcgcga
    11101 ggatcgggcg accaccagag gatgggggcg cctggagaaa accacggaaa aataccacgc
    11161 tggtaagcca ttaacaggcg cgcagggcta agatcgcccc ccagagccag ctggccgtta
    11221 ggctcacgta atgcgccttc cggggaaggg aaggctattg aatggcgaga aagctgaacc
    11281 aggcgcatga ccgcaaaact ccacgcaagt cggatcgttc aataatagct tacaaaccct
    11341 gcttaaactg gtaataacgc ccctgtctgg caagcagttc tgcgtgagta ccttgctcaa
    11401 taatttgccc gttgtccatc actattattt gttggaaacg agagagtccg cgaagtcgat
    11461 gggtgaccat taacaccgtt ttctcacgca tcatttctgc aagcaattca aggatctggc
    11521 tttcggttgt ggcatctaag ccttcggtag gttcatccag caacaccagt ggcgcatcat
    11581 gtaacagcgc acgggcgata gccagacggc gcagttcacc accggagagc tggcgtccgc
    11641 cttcacctaa ccaactgttg agacctgcgt cctcgagcag cttctccaga ccaacgcgac
    11701 gcaagatctc cgacagagcc tcatcactac tgccaggcga ggcgagtaac agattatcac
    11761 gcagcgtggc gctaaacaga tgcactcgct gaggaacaac gctgatggtc tgtcgtagag
    11821 ccgcttcatt caggctggct atggggctat cgttaagcaa aatctcgccc tgttgcgggt
    11881 cccaagcgcg ggtcagcagt tgtaacagtg ttgatttgcc gcatccggtt cgcccgagaa
    11941 tcgcaatatg ttccccggcg tttacctgaa gagaaatccc tttaagtgcc tgttgggatt
    12001 gctccggata agtgaactga acatcccgta atgtcagcga aacgcgatcg gcaacacgag
    12061 tttgggtatc agggaaggtg acttccggtt tttgatccgt taaatcagtg atccgaacgg
    12121 cagaggcaat gacttgcccc agatgctgaa atgcacccgt tactggtgcc agtgcttcaa
    12181 acgcggctaa cgcgcagaag acaaacaggg caattaacgc gccgggttga gcattgccgc
    12241 caacgccgcc agacgccatc cacagcatca ggatcaccgc taacgcgcca atgagcagca
    12301 ttatcgcttg cgacaatgcg gtcagttcag attgacggcg ttgcgcttcc agccattgaa
    12361 tttctgtatt ctctagttgc gtgcgataac gatcgctggc accaaaaatg gtcagctcag
    12421 cttgcccttg cagccaggcc gtcagttgtt ggcgatactg tccgcgaaga tgagtcagat
    12481 tttgcccggt gctttttccc gcacgataaa acagcggtgg catcaggaaa agcgtcagta
    12541 acataatgcc gcccagcgta aaggcgaggg tgaaatcaag gaaacttaac ccgactgtca
    12601 ccaccataat caccacaaaa gcgcccacca gcggcgagat aacgcgcagg taaagatgat
    12661 cgagcgtatc aacatccgcc accacgcgat tgagcaattc gccctgacga tagcgcgcca
    12721 gtccggcagg ggagaggggc aacaatttgc tgaaggtgta aatgcgcaga tgctgcaaca
    12781 cgcggaaagt cgcgtcgtga cttaccagac gttcaaaata acgcccggca gtacgggtga
    12841 ttgcggcacc acgcacgccc gcagcgggta gcatatagtt gaagctgtac agtccggcaa
    12901 cccccgcaac cgctgaggcc gagaggaacc agccggaaag tgtcaacaga ccgatactgg
    12961 cgagcagcgt cacaattgcc agcacaatac caagacttaa catccattta tgacgtttat
    13021 acagtgccag atagggtagc aaagcgcgca tttaaatctc ctcctgacga tgggccagta
    13081 atgtggcgaa tgggccacca gccacactta attccgcgta acgtccttgc tcaataatct
    13141 gaccatcctg cattacccaa atgacatccc agtcagcaag atcttctaac tggtgggtga
    13201 ccattaacgt tgtctggcgc agagaggcgg cattcagcgc ctccattacg cgctgttcac
    13261 tgtgagcatc aaggctggca gcgggttcat ccaacagtaa tagcgaacag ggatttagta
    13321 acgcacgggc caccgccacg cgctgcgcct gccccacgga aaggcgggca gcctggtcgc
    13381 caacaggcgt atcaacgcct tgtggcagga gcggtagaaa ctcgctgacc caggcgttat
    13441 ccagcgctgc ttgtaattca tgttcgctgg catcaggtcg cgccagtagt acgttatccc
    13501 gtaatgttgc tgccggtaat tgtggatttt gcccaaccca ggagagatgt ttacgccatg
    13561 attctggtga taaatcgcgt aattctatcc cgttgattcg tagcgatccc tgatatgaga
    13621 gaaaaccaga aagcgcgttc aacagtgaac ttttacctga accgctgcga ccaaccaaca
    13681 cggcacgttg gcctgctggc aaagtaaagt tcagcggtcc ggccagcgtt ttaccttccg
    13741 gcgacgtgat aaacagatcc tcggcctcaa tggtcaccgg atcggtcgat gctaattcca
    13801 cctcgccacg ctgcggatgg gcgagcgggg tttccataaa cgttttcaga ctgtcagctg
    13861 caccaacagc ctgggcttta gcatgataaa acgtaccgag atcgcgtaat ggctggaaaa
    13921 actctggcgc aaggatcagg gccagaaaac ccgcagccag cgtcacgcca gtatcgtagt
    13981 gaccaaaatc cagctcgccg agataggaaa aaccaaagta gaccgccacc agagcaattg
    14041 acagcgaggt aaaaaattcg agaatgccgg aggataaaaa tgccagccgt agcacttcca
    14101 ttgtccgttg gcggaaatct tccgaagcag aacgaatact ttcaatttca gcttcaccac
    14161 gaccaaaaat acgcaatgtt tccatgccgc gcaggcgatc gaggaaatgc ccacttaagc
    14221 gagcaagagc gagaaagtta cgtcggttag catcggcagc ccccattcca accagcgcca
    14281 taaacaacgg aattaacggt gcagtgccca gcagaatgag cgccgcagcc cagtttgagg
    14341 ggaagattgc caccacaatc agcaacggca ccgacactgc cagcgccatt tgcggcaggt
    14401 agcgtgcata gtaatcatgc atatcgtcaa tttgctcgag taccagcgtc gcccagctcc
    14461 ccgcaggttt accctgaatc cacgctggcc ctgcttgttg cagacggtcg agaacctgac
    14521 ggcggatggc aaagcggata tgctgcccgg cgtgataacc cacccgttcg cgtaaccaga
    14581 ccgcccatgc gcgcagtaca aaggtcagaa ccagtaacgt aaagggaagc agcagggctt
    14641 cacggggaat attctccata atcatatgtt gcagaattcg cgccatgaac caggcctggg
    14701 caatgatcaa tatgccgctt acaaagccca gcagacgaga aatattcagc caacgttggg
    14761 agatgacgct ttgctgtttt aaccagcggg ttaactcttt ttgacgagat ttattcattg
    14821 cacgcttagc aggtgagtta tcagaattat ttgcagagca atgttacaac ggggaaaaaa
    14881 taaaggcgac ccatagtcgc aaggtgtcgc cttctttact tttgttactg atttgtaaaa
    14941 ttattttgcg tcagctaaac catcgaggta gcgttccgca tcaagtgctg ccatgcagcc
    15001 agtaccggcc gaagtaatgg cctggcgata aatgtgatcc atcacgtcgc ctgcggcaaa
    15061 gacgccagga atgctggtct gggtggcatt accatgaata cccgactgta ctttgatgta
    15121 gccgttttcc agttccagct gcccttcgaa aatcgcagta ttcgggctgt gaccgatagc
    15181 aacaaacaga ccggcaacgt cgagtgactc gatgttatcg ctgttttgcg tatcgcgcag
    15241 acgaacgcca gtgacaccca tttgatcgcc ggtcacttct tccagcgtac ggttggtgtg
    15301 cagaatgatg ttgccgttct ccactttatc catcagacgc ttaataagga ttttttccgc
    15361 gcggaaaccg tcacggcggt gaatcagatg cacttccgaa gcgatgttag acagatacag
    15421 cgcctcttca accgcggtat tgccgccgcc gatgaccgca actttctggt tgcgatagaa
    15481 gaaaccgtcg caggttgcac aagcagaaac cccacggcct ttaaaggctt cttcagaggg
    15541 caggccgaga tagcgtgcag aagctccggt ggcaataatc agcgcgtcgc aagtgtattc
    15601 gccgttatcg ccattcagac ggaacggacg gttttgcaga tccaccttgt tgatatgatc
    15661 aaaaatgatc tcagtttcaa acttggtggc atgttcgtgc atgcgctcca ttaataacgg
    15721 accggtcaga tcgtttggat caccaggcca gttttccact tccgtggtgg tggtcagttg
    15781 gccgcctttt tccatgccgg taatcagcac aggttgcagg ttggcgcgcg ccgcgtagac
    15841 agcagcggtg tatcccgccg ggcctgaacc caggataagc agtttactgt gtttggtcgt
    15901 gcccatgaga tccccatagt tgttggcaga caatgggcag gattgtaggg aatttacaga
    15961 cgtaaaaaaa gagtatgacg attttgttaa caatttgtgc aatcggcagc atcgataagc
    16021 aggtcaaatt ctcccgtcat tatcacctct gctacttaaa tttcccgctt tataagccga
    16081 ttaaatgatg aataaacgcc cctgttaatg aatatctggc atgttgtact aaaaatcgat
    16141 gttttgcttt gacaatcccc tggtgttttg cgaaaacatt cgaggaagaa aaaaaacagt
    16201 attcttatat gcgcataacc atgcatgtaa ataccatgtt taccgtgcta gtgaaatcta
    16261 cgtatggcgt ggacagacgc cattcgtgat gtcgatagct gccacaaggc aacggtcttc
    16321 tcaccgtaga cccaggcatt gcgcgccgtg aatcttcatg atttcggtct atcgtgacgg
    16381 gtagcgactc tgaacagtga tgtttcaggg gcagacagga gtagggaagg aatacagaga
    16441 gacaataata atggtagata gcaagaagcg ccttggcaaa gatctcgacc gtatcgatcg
    16501 taacattctt aatgagttgc aaaaggatgg gcgtatttct aacgtcgagc tttctaaacg
    16561 tgtgggactt tccccaacgc cgtgccttga gcgtgtgcgt cggctggaaa gacaagggtt
    16621 tattcagggc tatacggcgc tgcttaaccc ccattatctg gatgcatcac ttctggtatt
    16681 cgttgagatt actctgaatc gtggcgcacc ggatgtgttt gaacaattca ataccgctgt
    16741 acaaaaactt gaagaaattc aggagtgtca tttagtatct ggtgatttcg actacctgtt
    16801 gaaaacacgc gtgccggata tgtcagctta ccgtaagttg ctgggggaaa ccctgctgcg
    16861 tctgcctggc gtcaatgaca cacggacata cgttgttatg gaagaagtca agcagagtaa
    16921 tcgtctggtt attaagacgc gctaacacgg aacaggtgca aaatcggcgt attttgatta
    16981 cactcctgtt aatccataca gcaacagtac tggggtaacc tggtactgtt gtccgtttta
    17041 gcatcgggca ggaaaagcct gtaacctgga gagcctttct tgagccagga atacactgaa
    17101 gacaaagaag tcacattgac aaagttaagt agcggccgcc gccttctgga ggcgttgctg
    17161 atccttattg tcctgtttgc cgtctggttg atggctgcct tactaagctt taacccttcg
    17221 gaccccagct ggtcgcaaac ggcctggcat gaacctatcc ataatttagg tgggatgccc
    17281 ggtgcgtggt tggcagatac gctgttcttt atttttggcg tgatggctta caccattccc
    17341 gtcattattg tcggcggttg ttggtttgcc tggcgtcatc agtccagcga cgaatacatt
    17401 gattattttg ccgtttcgct acgcatcatt ggcgttttgg cgctcatcct tacctcctgt
    17461 ggtctggcgg caatcaacgc tgacgatatc tggtattttg cctccggtgg cgtcattggc
    17521 agcttactaa gcactacgct acaaccactg ctacacagta gcgggggaac tattgcgctg
    17581 ctctgcgttt gggcagcggg cctgacgttg ttcaccggtt ggtcatgggt gaccattgct
    17641 gaaaaactcg gcggctggat tttaaacatt ctcaccttcg ccagtaatcg tacccgtcgc
    17701 gatgatacct gggtcgatga agatgagtat gaagacgacg aagagtatga agatgaaaat
    17761 cacggcaaac agcatgaatc acgccgtgcc cgtattcttc gcggcgcgct agcgcgtcgt
    17821 aaacggttgg cggaaaaatt cattaatccg atggggcggc aaacagacgc tgcgttgttc
    17881 tccggtaagc ggatggatga tgacgaagag attacctaca ctgcacgcgg tgtggctgcc
    17941 gaccctgacg acgtcctatt ttcgggcaat cgtgcaacgc agccagaata tgacgaatac
    18001 gatccattat taaacggtgc gccaattacc gaacctgtcg ctgtagcagc tgctgctacc
    18061 acggcggcac aaagctgggc tgcgccggtt gaacctgtga ctcagacgcc gcctgttgcc
    18121 tctgttgatg ttccaccttc gcaacctaca gtagcctggc agcctgtacc gggtccacaa
    18181 acgggagagc cggttattgc tcctgcaccg gaaggttacc cacagcagcc acaatatgcg
    18241 cagcctgcag tgcaatataa tgagccgctg caacaaccag tacagccaca gcagccgtat
    18301 tatgcacctg cagctgaaca acctgtgcaa caaccttact acgctcctgc ggcggaacaa
    18361 cctgttcagc aaccgtatta cgcccctgcg ccagaacaac cggtggcagg taacgcctgg
    18421 caagccgaag agcagcaatc cacttttgct ccacagtcta cataccagac tgagcaaact
    18481 tatcagcaac cagccgctca ggagccgttg taccaacagc cgcaacccgt tgaacagcag
    18541 cctgttgtgg agcctgaacc cgttgtagaa gagacaaaac ccgcgcgtcc gccactttac
    18601 tattttgaag aagtggaaga gaagcgagcc cgtgaacgtg aacaacttgc ggcctggtat
    18661 caaccgattc cagaaccggt taaagaacca gaaccgatca aatcttcgct gaaagcacct
    18721 tctgttgcag cagtacctcc agtagaaacc gctgccgctg tttctccgct ggcatctggc
    18781 gtgaaaaaag cgacactggc gacgggggct gccgcaaccg ttgccgcgcc agtcttcagt
    18841 ctggcaaata gcggtggacc gcgtccgcag gtcaaagagg ggattggccc gcagttgcca
    18901 cgaccgaaac gtatccgcgt gccaactcgc cgtgaattgg cgtcttacgg tattaagctg
    18961 ccctcacagc gtgcggcgga agaaaaagcc cgtgaagccc agcgcaatca gtacgattct
    19021 ggcgatcagt acaacgatga tgaaatcgat gcgatgcagc aggatgaact ggcacgtcag
    19081 ttcgcccaga cacagcagca acgctatggc gaacagtatc aacatgatgt gcccgtaaac
    19141 gcagaagatg cagatgctgc ggcagaggct gaactggctc gtcagtttgc gcaaactcaa
    19201 caacaacgtt attccggcga acaaccggct ggggcgaatc cgttctcgct ggatgatttt
    19261 gaattttcgc caatgaaagc gttgctggat gatggtccac acgaaccgtt gtttacgcca
    19321 attgttgaac ctgtacagca gccgcaacaa ccggttgcac cgcagcagca gtatcagcag
    19381 ccacaacagc cggttgcgcc acagcagcaa tatcagcagc cgcaacagcc ggttgcgccg
    19441 cagccacaat atcagcagcc gcaacaaccg gttgcgccac agcagcaata tcagcagccg
    19501 caacaaccag ttgcgccgca gccgcagtat caacagccgc aacaaccggt tgcgccgcag
    19561 cagcagtatc agcagccaca acagccagtt gcgccacaac cgcaggatac cctgcttcat
    19621 ccgctgttga tgcgtaatgg cgacagccgt ccgttgcata aaccgacgac gccgctgcct
    19681 tctctggatt tgctgacacc gccgccgagc gaagtggagc cggtagatac ctttgcgctt
    19741 gaacaaatgg ctcgcctggt ggaagcgcgt ctggctgatt tccgtattaa agccgatgtt
    19801 gtcaattact ctccggggcc ggttatcact cgctttgaat tgaacctggc accgggcgta
    19861 aaagcggcgc gcatttctaa cttgtcacgg gaccttgccc gttcactttc gacggtggcg
    19921 gtgcgtgtcg ttgaagttat tcctggcaaa ccctatgtag gtctggagtt accgaataaa
    19981 aaacgacaaa ccgtttatct gcgcgaagtt ttggataacg ccaaattccg cgataatccg
    20041 tcgccattaa ccgtggtgct gggtaaagat atcgccggtg agccggtggt tgccgatctg
    20101 gcgaaaatgc cgcacttgtt ggttgcgggg actaccggtt ccggtaaatc tgtcggtgtg
    20161 aacgcgatga tcctgagcat gctttataaa gcacagccag aagatgtgcg tttcatcatg
    20221 atcgacccga aaatgctgga gctttcggtt tatgaaggca ttccgcatct gttaacggaa
    20281 gtcgttactg atatgaaaga tgccgccaac gcgctgcgct ggtgtgttaa cgagatggag
    20341 cgtcgttata aactgatgtc tgcgctgggt gtgcgtaatc tggcgggtta taacgaaaaa
    20401 attgctgaag ccgatcgcat gatgcgtccg attccagacc cgtactggaa gccgggtgac
    20461 agtatggatg cccagcatcc ggtgctgaaa aaagaaccat acattgtggt gttggttgac
    20521 gaatttgccg acctgatgat gacggtaggt aaaaaagtgg aagagctgat agcacgtctg
    20581 gcgcaaaaag cccgtgccgc gggtatccac ctcgtactgg caactcagcg tccatcggtt
    20641 gatgttatta ctggtctgat taaagcgaat attccgaccc gtatcgcctt taccgtatcc
    20701 agtaagattg actcacgtac cattcttgat caggctggcg cggaatcact gctggggatg
    20761 ggggatatgc tctactctgg gccgaactcc acgttgccgg tacgtgtcca tggtgctttt
    20821 gttcgcgatc aggaagttca tgccgtggtg caggactgga aagcgcgtgg tcgcccacag
    20881 tatgttgatg gcatcacttc cgacagtgaa agcgaaggtg gcgcgggcgg tttcgatggc
    20941 gctgaagaac tggatccgtt gttcgatcag gcggtgcagt ttgtcactga aaaacgcaaa
    21001 gcgtcaattt ctggcgtaca gcgtcagttc cgcattggtt ataaccgtgc agcgcgtatt
    21061 atcgaacaga tggaagcgca ggggattgtc agcgaacagg ggcacaacgg taatcgtgaa
    21121 gtgctggctc caccgccgtt tgactaatta atgcattgcc ggataaggcg cggtagcgtc
    21181 gcatccggca ctctatcaac tgaaaattca gtattttctt ctttcctcaa gctgattatt
    21241 agcctggaat agagagtaga gggaactccc gatcgggagt gacgtaattt gaggaataat
    21301 gatgaaaaaa attgccatca cctgtgcatt actctcaagc ttagtagcaa gcagcgtttg
    21361 ggctgatgcc gcaagcgatc tgaaaagccg cctggataaa gtcagcagct tccacgccag
    21421 cttcacacaa aaagtgactg acggtagcgg cgcggcggtg caggaaggtc agggcgatct
    21481 gtgggtgaaa cgtccaaact tattcaactg gcatatgaca caacctgatg aaagcattct
    21541 ggtttctgac ggtaaaacac tgtggttcta taacccgttc gttgagcaag ctacggcaac
    21601 ttggctgaaa gatgccaccg gtaatacgcc gtttatgctg attgcccgca accagtccag
    21661 cgactggcag cagtacaata tcaaacagaa tggcgatgac tttgtcctga cgccgaaagc
    21721 cagcaatggc aatctgaagc agttcaccat taacgtggga cgtgatggca caatccatca
    21781 gtttagcgcg gtggagcagg acgatcagcg cagcagttat caactgaaat cccagcaaaa
    21841 tggggctgtg gatgcagcga aatttacctt caccccgccg caaggcgtca cggtagatga
    21901 tcaacgtaag tagaggcacc tgagtgagca atctgtcgct cgatttttcg gataatactt
    21961 ttcaacctct ggccgcgcgt atgcggccag aaaatttagc acagtatatc ggccagcaac
    22021 atttgctggc tgcggggaag ccgttgccgc gcgctatcga agccgggcat ctgcattcta
    22081 tgatcctctg ggggccaccg ggtaccggca aaacaaccct cgctgaagtg attgcccgct
    22141 atgcgaacgc tgatgtggaa cgtatttctg ccgtcacctc tggcgtgaaa gagattcgcg
    22201 aggcgatcga gcgcgcccgg caaaaccgca atgcaggtcg ccgcactatt ctttttgttg
    22261 acgaagtgca ccgattcaac aaaagccagc aggatgcatt tctgccacat attgaagacg
    22321 gcaccatcac ttttattggc gcaaccactg aaaacccgtc gtttgagctt aattcggcac
    22381 tgctttcccg tgcccgtgtc tatctgttga aatccctggg tacagaggat attgagcaag
    22441 tactaactca ggcgatggaa gacaagaccc gtggttatgg tggtcaggat attgttctgc
    22501 cagatgaaac acgacgcgcc attgctgaac tggtgaatgg cgacgcgcgc cgggcgttaa
    22561 atacgctgga aatgatggcg gatatggccg aagtcaatga tagcggtaag cgggtcctga
    22621 agcctgaatt attgaccgaa atcgccggtg agcgaagcgc ccgctttgat aacaaaggcg
    22681 atcgctttta cgatctcatt tccgcactgc ataagtcggt acgtggtagc gcacccgatg
    22741 cggcgctcta ctggtatgcg cgaattatta ccgctggtgg cgatccgtta tatgtcgcgc
    22801 gtcgttgcct ggcaattgct tccgaagatg tcggcaatgc cgacccacgg gcgatgcagg
    22861 tggcaattgc ggcctgggat tgctttactc gcgttggccc ggcggaaggt gaacgcgcca
    22921 ttgctcaggc gattgtttac ctggcctgcg cgccaaaaag caacgctgtc tacactgcgt
    22981 ttaaagctgc gctggcggat gctcgcgaac gcccggatta tgacgtgccg gttcatttgc
    23041 gtaatgcgcc gacgaaatta atgaaggaaa tgggctacgg gcaggaatat cgttacgctc
    23101 atgatgaagc aaacgcttat gctgccggtg aggtttactt cccgccggaa atagcacaaa
    23161 cacgctatta tttcccgaca aacaggggcc ttgaaggcaa gattggcgaa aagctcgcct
    23221 ggctggctga acaggatcaa aatagcccca taaaacgcta ccgttaatgt tatcgttgcg
    23281 gtaatgttgt tactgtatcc ctgtggtcgc aggctgtggc cacatctccc atttaattcg
    23341 ataagcacag gataagcatg ctcgatccca atctgctgcg taatgagcca gacgcagtcg
    23401 ctgaaaaact ggcacgccgg ggctttaagc tggatgtaga taagctgggc gctcttgaag
    23461 agcgtcgtaa agtattgcag gtcaaaacgg aaaacctgca agcggagcgt aactcccgat
    23521 cgaaatccat tggccaggcg aaagcgcgcg gggaagatat cgagccttta cgtctggaag
    23581 tgaacaaact gggcgaagag ctggatgcag caaaagccga gctggatgct ttacaggctg
    23641 aaattcgcga tatcgcgctg accatcccta acctgcctgc agatgaagtg ccggtaggta
    23701 aagacgaaaa tgacaacgtt gaagtcagcc gctggggtac cccgcgtgag tttgactttg
    23761 aagttcgtga ccacgtgacg ctgggtgaaa tgcactctgg cctcgacttt gcagctgcag
    23821 ttaagctgac tggttcccgc tttgtggtaa tgaaagggca gattgctcgc atgcaccgcg
    23881 cactgtcgca gtttatgctg gatctgcata ccgaacagca tggctacagt gagaactatg
    23941 ttccgtacct ggttaaccag gacacgctgt acggtacggg tcagctgccg aaatttgctg
    24001 gcgatctgtt ccatactcgt ccgctggaag aagaagcaga caccagtaac tatgcgctga
    24061 tcccaacggc agaagttccg ctgactaacc tggtgcgcgg tgaaatcatc gatgaagatg
    24121 atctgccaat taagatgacc gcccacaccc catgcttccg ttctgaagcc ggttcatatg
    24181 gtcgtgacac ccgtggtctg atccgtatgc accagttcga caaagttgaa atggtgcaga
    24241 tcgtgcgccc ggaagactca atggcggcgc tggaagagat gactggtcat gcagaaaaag
    24301 tcctgcagtt gctgggcctg ccgtaccgta aaatcatcct ttgcactggc gacatgggct
    24361 ttggcgcttg caaaacttac gacctggaag tatggatccc ggcacagaac acctaccgtg
    24421 agatctcttc ctgctccaac gtttgggatt tccaggcacg tcgtatgcag gcacgttgcc
    24481 gcagcaagtc ggacaagaaa acccgtctgg ttcataccct gaacggttct ggtctggctg
    24541 ttggtcgtac gctggttgca gtaatggaaa actatcagca ggctgatggt cgtattgaag
    24601 taccagaagt tctgcgtccg tatatgaacg gactggaata tattggctaa tacccaattt
    24661 ttctgaatct aaaaagcgcc tgcgggcgct ttttttgtct ccctttgata ccgaacaata
    24721 attactcctc acttacacgt aatactactt tcgagtgaaa atctacctat ctctttgatt
    24781 ttcaaattat tcgatgtata caagcctata tagcgaactg ctatagaaat aattacacaa
    24841 tacggtttgt tactggaatc aatcgtgagc aagcttgagt gagccattat gaaaacgaaa
    24901 atccctgatg cggtattggc tgctgaggtg agtcgccgtg gtttggtaaa aacgacagcg
    24961 atcggcggcc tggcaatggc cagcagcgca ttaacattac cttttagtcg gattgcgcac
    25021 gctgtcgata gcgccattcc aacaaaatca gacgaaaagg ttatctggag cgcctgtaca
    25081 gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg tggacggtga aatcaaatat
    25141 gtcgaaacgg acaataccgg cgatgacaat tacgacggcc tgcaccaggt tcgcgcctgc
    25201 ctgcgtgggc gttccatgcg tcgccgtgtc tacaatccgg accgcctgaa atatccgatg
    25261 aaacgagtcg gggcgcgcgg tgaaggcaaa ttcgagcgca ttagctggga agaagcctac
    25321 gacatcatcg cgaccaatat gcagcgcctg atcaaagagt acggcaacga gtctatctat
    25381 ctgaactatg gcaccggtac gctgggcggc accatgaccc gctcctggcc gccgggaaat
    25441 accctggtcg cgcggctgat gaactgctgc ggcggctatc tgaaccatta cggcgactac
    25501 tcctccgcgc aaattgcgga aggtttgaac tatacctacg gcggctgggc agatggcaac
    25561 agcccgtcgg atatcgaaaa cagtaagctg gtagtgctgt ttggtaataa ccctggcgaa
    25621 acgcgaatga gtggcggtgg ggtgacttac tatcttgaac aggcacgcca gaaatctaat
    25681 gcccgcatga tcatcatcga tccgcgctat accgacaccg gtgccgggcg cgaagatgag
    25741 tggatcccta ttcgtccggg aacagatgcc gcactggtta acggtctggc gtacgtcatg
    25801 atcactgaaa acctggtgga tcaggcattc ctcgataaat attgcgttgg ctacgatgag
    25861 aaaaccctgc cagccagtgc gccgaaaaat ggccactata aagcttatat tctgggtgaa
    25921 gggccagatg gcgtggctaa aacaccggaa tgggcctcac aaatcaccgg tgttccggca
    25981 gacaaaatca tcaaactggc tcgtgaaatc ggcagtacca aaccggcgtt tatcagccag
    26041 ggatggggcc cgcagcgtca cgctaacggt gaaatcgcaa cccgtgctat ctcgatgctg
    26101 gcgattctga ccggtaacgt tggtattaat ggagggaaca gcggcgcgcg tgaaggttca
    26161 tacagcttac cgtttgtccg tatgccgacc ttggaaaacc cgatccagac cagcatttcg
    26221 atgtttatgt ggaccgatgc cattgaacgt ggcccggaaa tgacggcgct gcgtgatggt
    26281 gtacgcggga aagataagct ggatgtgccg atcaaaatga tctggaacta tgccggtaac
    26341 tgcctggtta accagcattc tgaaatcaac cgtacccatg aaatccttca ggatgataag
    26401 aagtgcgagc tgattgtggt tatcgactgc cacatgacct catcggcgaa atatgctgac
    26461 atcctgctgc ctgactgcac cgcttccgaa cagatggact ttgcgctgga tgcatcctgc
    26521 gggaatatgt cttacgtgat tttcaacgat caggtgatta aaccgcgctt tgaatgtaag
    26581 accatctatg aaatgaccag cgaactggca aaacgtcttg gcgttgagca acagtttact
    26641 gaaggccgta cccaggaaga gtggatgcgg catctgtatg cccagtcgcg ggaagcgatt
    26701 cctgaactgc caacgtttga agagttccgc aagcagggga tctttaaaaa gcgcgaccca
    26761 caagggcatc acgttgctta taaagccttc cgtgaagatc cgcaggcaaa cccactgact
    26821 acgccatcgg gcaaaattga gatttattcg caggcgctgg ctgacattgc cgctacctgg
    26881 gaattgccag aaggcgatgt gatcgatcca ctgccgatct acacgccggg ctttgaaagt
    26941 tatcaggatc cgctgaacaa acagtatccg ctgcagctta cgggtttcca ctataaatct
    27001 cgcgttcact caacttacgg caacgttgat gtgctgaaag cagcttgccg tcaggaaatg
    27061 tggatcaacc cgcttgatgc gcaaaaacgc ggtatcaaca acggcgataa agtcaggatc
    27121 tttaacgatc gtggtgaagt tcatattgag gcgaaagtga cgccacgaat gatgccaggt
    27181 gtggtcgcac tgggtgaagg tgcctggtat gacccggatg caaaacgtgt cgataagggt
    27241 ggttgtatta acgtactgac cactcaacgt ccgtctcctc tcgctaaggg gaatccgtca
    27301 catacaaacc ttgttcaggt tgaaaaggtg taaggagtaa ccgatgacaa cccagtatgg
    27361 attttttatt gattccagcc gttgcaccgg ttgcaaaacc tgcgagctgg cttgtaaaga
    27421 ctacaaagat ttgacgccag aagtcagctt ccgccgcatt tatgaatatg ctggcggcga
    27481 ctggcaggaa gataacggtg tctggcacca gaacgtgttt gcctactatc tgtcgatttc
    27541 gtgcaaccac tgcgaagatc cggcttgtac taaagtctgc ccgagcggtg cgatgcataa
    27601 acgtgaagat ggttttgttg tggtcgatga agatgtgtgc attggctgcc gctactgcca
    27661 tatggcttgc ccgtatggcg caccgcaata taacgaaacg aaaggccata tgaccaaatg
    27721 cgatggttgt tatgaccgtg ttgcggaggg taaaaagccg atctgtgttg aatcttgtcc
    27781 gctgcgggcg ctggatttcg gccctatcga cgagctgcgt aaaaaacatg gcgatctggc
    27841 ggccgttgcg ccgttgccgc gagctcactt taccaaaccg aatattgtga tcaaacccaa
    27901 tgccaatagc cgcccgaccg gggataccac tggctatctg gcaaacccga aggaggtgtg
    27961 agatgggaag tggatggcat gaatggccgc tgatgatctt cacggtcttc gggcaatgtg
    28021 tggcgggtgg ttttatcgtt ctggctttgg cgctgctcaa aggcgacctg cgagcagaag
    28081 cccagcagcg tgttatcgcc tgcatgtttg gcttatgggt gctgatgggc attggcttta
    28141 tcgcttctat gctccatctt ggttcaccga tgcgcgcttt taactcgctc aaccgggtag
    28201 gggcttcagc actcagtaac gaaatcgcca gcggttcgat cttttttgcc gtaggcggca
    28261 tcggctggtt gctggcaatg ctgaaaaagc tgtcaccggc attgcgtacg ctttggctgg
    28321 tggtgacgat ggttcttggc gtcgtctttg tctggatgat ggtgcgtgtg tataacagca
    28381 ttgataccgt tccgacctgg tacagcatct ggacgccgat gggcttcttc ctgacgatgt
    28441 tcatgggcgg cccgttactg gggtacctgt tgctgagtct ggctggcgtc gatagctggg
    28501 cgatgcgtct gctgccagcc atttccgtac tggcactggt ggtgagtggc gtggtgtcag
    28561 tgatgcaggg cgcagagctg gcgactattc atagttctgt gcagcaggcc gcagcgctgg
    28621 tgccggacta tggtgcgctg atgtcctggc ggatcgtgct tttggccgtt gccctgtgct
    28681 tgtggattgc accacagcta aaaggttatc agcctgcggt tccgctacta tccgtctcat
    28741 tcattctgct tctggcaggg gaattaatcg gtcgcggcgt attctatggt ttgcatatga
    28801 ccgtggggat ggccgtcgca agctaatcag taacaaccgg ggtttcggcc ccggttctct
    28861 tttatttctg cttcgttaac gtgtcataac tggtcatcaa attacgataa tcaggaatat
    28921 ggttagagaa caacgtcgcc agtccttcaa tatcattacg ccagtcacgg tgcagctcgc
    28981 aagccacgcc gaaccatgtc atcaattgcg cacctgcttg cgacatgcga tcccatgccg
    29041 aatgccgggt aatttcatta aaggtccctg acgcgtcagt cacaacaaac acatcaaacc
    29101 cttcttcaat ggctgaaagc gccgggaatg ccacgcaaac ttcggttacc acaccggcaa
    29161 taattaactg ttttttacct gtcgctttga ctgcttttac aaaatcttcg ttatcccagg
    29221 cgttaatatt gcccgggcga gcaatgtaag gtgcatcggg gaattgtgct ttcagttctg
    29281 gaactaatgg gccgttagga ccggtttcaa aactggtggt aagaatagtt ggtaagttga
    29341 aatacttagc caggtcaccc agcgccagca cgttattttt aaacttatcg ggttcgatat
    29401 cccgtacaag ggaaagtaaa ccagcctggt gatcaacaag caaaacagca gcatcatttt
    29461 tatcaagacg aacatacggt ttggtcatcc tcttctcctt tcgagagtca gtggtacgcc
    29521 tgaaatcggt cagacgtgat gcattgctct gaaagcatag acgggaaata tgagtttgct
    29581 gtgaccatga aatttttcga ctgaacgaag cgtatgaaat ttgtgttaga tcaataaaaa
    29641 caatcagatg agattaatca tctcttcagt attagcgtta tgaatgagta atataaatcg
    29701 ataataccgc cgttgcgata tgcgcgtatt ttcacccatt gacaatgttt ttggcggtgg
    29761 catgatgcgc atgaaatttg aacttcctca cggttttaat tcatgtccac gtatacccgg
    29821 cctgtcatgc ttttgctgtc tggcctgctt ttgttgactc tggcgatcgc ggtgttaaat
    29881 acactcgtgc cgctttggct cgcccaggaa cacatgtcca catggcaggt aggcgttgtc
    29941 agctcatcct attttaccgg caaccttgtc ggtacattgc tgacagggta tgtcattaaa
    30001 cgcattggtt ttaaccgcag ctattatctg gcctccttca tttttgccgc tggctgtgcc
    30061 ggccttggcc tgatgattgg attctggagt tggttggcgt ggcgttttgt cgcgggcgtc
    30121 ggctgtgcca tgatttgggt agtcgttgag agcgcgctga tgtgcagtgg gacgtcacgt
    30181 aaccgtgggc gtttgcttgc tgcatacatg atggtttatt acgtgggaac gtttttaggc
    30241 cagttactgg tcagcaaggt ctcaaccgag ctgatgagcg tattgccgtg ggttacaggt
    30301 ttgacattgg cagggatctt accgctgttg tttacgcgtg tcctgaatca gcaggctgaa
    30361 aaccatgatt cgacgtcaat tacggcaatg ctaaaactcc gtcaggcgcg gcttggcgtg
    30421 aatggctgca ttatctcagg aatcgttctg ggatctctat atggcctgat gcctctgtac
    30481 ctcaatcaca aaggggtgag caatgccagt attggtttct ggatggcggt actggtcagt
    30541 gcgggtatcc tcggacaatg gccgattgga cgtctggcgg ataagtttgg tcgactgctg
    30601 gttttgcgtg ttcaggtctt tgtcgtcatt ctcggcagta ttgcgatgct tagccaggcg
    30661 gcgatggccc cagcgttatt catcctcggt gccgctggct ttacgctata tccggtggcg
    30721 atggcatggg cttgcgagaa agttgaacat catcaactgg tggcgatgaa ccaagcctta
    30781 ctgttgagct atactgtggg aagtctgctt ggcccgtcat ttaccgctat gctaatgcag
    30841 aatttctccg ataatttatt gtttatcatg atcgccagcg tatcgtttat ctatttgctg
    30901 atgctgctgc gcaacgccgg tcatacgccg aaacccgttg ctcacgtgta aatgaattca
    30961 agcagagtgt gaacttactg tttcacactc tgcttttttg tttcttctat ctgacttgct
    31021 ttattccaaa ttttattcgt ttaaaaataa aatgtgtagc aggttataat tttgcatttc
    31081 gctatttccg cacttcttat ttgccgcgca taatccctcg ttttacagat gcccctataa
    31141 ttttggcgaa ggatttgtct atggctggga atgttcagga aaaacagctg cgatggtaca
    31201 acattgcgct gatgtctttt atcactgtct ggggttttgg caacgttgtt aataactacg
    31261 ccaaccaggg gctggtggtt gttttttcat gggtgtttat ctttgcgctc tatttcacac
    31321 cttatgcgct aattgttggt cagttaggct cgaccttcaa agatgggaag ggcggggtca
    31381 gtacctggat taaacacacg atgggacccg gactggctta tctcgccgcg tggacctact
    31441 gggtggtgca tattccctat ctggcacaaa aaccccaggc aattctgatt gcgctcggtt
    31501 gggcgatgaa aggcgacggt tcgctaatca aagaatattc agtcgtagcg ttacaggggt
    31561 taacgctggt gctgtttatc ttctttatgt gggttgcttc acgcggtatg aaatcgctga
    31621 aaatcgtcgg ttctgtggca gggattgcta tgtttgttat gtcgctcctg tatgtggcga
    31681 tggcggtaac cgcgcctgca attactgaag tgcatattgc gaccacaaac attacctggg
    31741 aaacgttcat tcctcatatc gactttacct acattaccac tatttcaatg ctggttttcg
    31801 cggttggcgg agcagagaag atttctcctt acgttaatca aacgcgcaac ccaggaaaag
    31861 aatttccaaa agggatgtta tgcctggcgg tgatggttgc ggtttgtgcc attctgggct
    31921 cgctggcgat ggggatgatg tttgattcgc gtaatatccc ggatgactta atgaccaacg
    31981 gtcagtatta cgcctttcag aagctgggcg agtattacaa catgggtaat actttaatgg
    32041 tgatttacgc cattgcgaat accctgggac aagtagcggc gctggtattc tcgattgatg
    32101 ccccgcttaa agtgctatta ggtgatgctg acagcaaata tattccagcc agtttatgtc
    32161 gtaccaacgc ttctggtacg cccgttaatg gctattttct gaccctggta ctggtggcga
    32221 ttctgattat gctgccgact ctcggcattg gtgatatgaa caatctctat aaatggctgt
    32281 tgaaccttaa ttcggtagtg atgccgctgc gttatttatg ggtatttgtt gcatttattg
    32341 cagtcgttcg cctggcgcag aaatataaac cagaatatgt ctttattcgt aataagcctc
    32401 tggcaatgac cgtcgggatt tggtgttttg cctttaccgc ctttgcctgt ttgacgggga
    32461 tcttcccgaa aatggaagcc ttcactgcag agtggacctt ccagttggcg ctgaatgttg
    32521 caacgccgtt tgtgctggta ggattaggac tgatattccc gctgctggcg cgtaaagcga
    32581 atagtaaata attattgtgg tcggtgacgc tgactctggc agatctgacc cgtttttaca
    32641 taatcgagaa atgcgcgcaa gccgcaggac atatactggc ggtttgggta atagatctgg
    32701 aagcctggac gctctgtgat ccagtcttcc agtacacatt ctaaacgacc ggtatcaagg
    32761 tactccttga tctgttcata gagcagatac ccgataccag cacccatcaa aacggcatcc
    32821 agttcagcat caacatca
//
LOCUS       DAESHI010000039        32648 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.039, whole genome shotgun sequence.
ACCESSION   DAESHI010000039 DAESHI010000000
VERSION     DAESHI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32648
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.039"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            78..1580
                     /locus_tag="KW011_001881"
     CDS             78..1580
                     /locus_tag="KW011_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBH7660222.1"
                     /translation="MNKKLYLFPDSNIFLQCKDLTQVNFSEITACDEVCIIITRPIQQ
                     EIDRQKGQGNSRLSKKARKAASLFNQVVDSTDMTLVIRERSPRVFLTMDLSLKPCEQL
                     SEQLDYQEADDRFVGIAAGYCADDPDSEVAIITNDSGPRFSAIKHNIICYKVPSSWLL
                     PAEPDEKDKQIKLLEAKLKQFTQSMPDFSIKVADDEQLNIVLPYYTALSEYEVDNLLC
                     QLTDAFPLVTNFDNEQTTPQSLEKIIKSFSQQEYFPVSADDISLYKEKHYPLWKKQCE
                     EILKNCHTHINPKAINYPLSFLLQNTGYTIAERAVVTFTAKGDFRLCGFNWEIMEELT
                     ESKRIDLPSAPTAPKGKWRSVCGVTERMPYAGGALETPLSSIISKEIGPLLSLTPTER
                     DKNDFYYKEKRNEPVQVVSLECEEWRHQVKEEIFPLFVYTANKPKKVNGAIEVRVDAN
                     NLTNPVIKTFKLKITIEEHSAFSTITEMVQTYIQEQKKRQKSSISAQIKT"
     gene            1824..2084
                     /locus_tag="KW011_001882"
     CDS             1824..2084
                     /locus_tag="KW011_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HBH7660223.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGVHSSEDIPWHDLPASHRGNNQMSLPLPQN"
     gene            2275..2415
                     /locus_tag="KW011_001883"
     CDS             2275..2415
                     /locus_tag="KW011_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HBH7660224.1"
                     /translation="MSQKSLTTITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(2722..3021)
                     /gene="ihfA"
                     /locus_tag="KW011_001884"
     CDS             complement(2722..3021)
                     /gene="ihfA"
                     /locus_tag="KW011_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HBH7660225.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(3026..5413)
                     /gene="pheT"
                     /locus_tag="KW011_001885"
     CDS             complement(3026..5413)
                     /gene="pheT"
                     /locus_tag="KW011_001885"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBH7660226.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            complement(5428..6411)
                     /gene="pheS"
                     /locus_tag="KW011_001886"
     CDS             complement(5428..6411)
                     /gene="pheS"
                     /locus_tag="KW011_001886"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBH7660227.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     misc_feature    complement(6620..6744)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(6694..6738)
                     /gene="pheM"
                     /locus_tag="KW011_001887"
     CDS             complement(6694..6738)
                     /gene="pheM"
                     /locus_tag="KW011_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HBH7660228.1"
                     /translation="MNAAIFRFFFYFST"
     gene            complement(6861..7217)
                     /gene="rplT"
                     /locus_tag="KW011_001888"
     CDS             complement(6861..7217)
                     /gene="rplT"
                     /locus_tag="KW011_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBH7660229.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            complement(7270..7467)
                     /gene="rpmI"
                     /locus_tag="KW011_001889"
     CDS             complement(7270..7467)
                     /gene="rpmI"
                     /locus_tag="KW011_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBH7660230.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            complement(7564..8106)
                     /gene="infC"
                     /locus_tag="KW011_001890"
     CDS             complement(7564..8106)
                     /gene="infC"
                     /locus_tag="KW011_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBH7660231.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            complement(8110..10038)
                     /gene="thrS"
                     /locus_tag="KW011_001891"
     CDS             complement(8110..10038)
                     /gene="thrS"
                     /locus_tag="KW011_001891"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBH7660232.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            10563..12461
                     /gene="espL1"
                     /locus_tag="KW011_001892"
                     /pseudo
     CDS             10563..12461
                     /gene="espL1"
                     /locus_tag="KW011_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            12633..12740
                     /locus_tag="KW011_001893"
     CDS             12633..12740
                     /locus_tag="KW011_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660233.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(12793..13551)
                     /locus_tag="KW011_001894"
     CDS             complement(12793..13551)
                     /locus_tag="KW011_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HBH7660234.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            13838..14767
                     /gene="pfkB"
                     /locus_tag="KW011_001895"
     CDS             13838..14767
                     /gene="pfkB"
                     /locus_tag="KW011_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HBH7660235.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            14868..15158
                     /gene="ghoS"
                     /locus_tag="KW011_001896"
     CDS             14868..15158
                     /gene="ghoS"
                     /locus_tag="KW011_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBH7660236.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            15264..16124
                     /locus_tag="KW011_001897"
     CDS             15264..16124
                     /locus_tag="KW011_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBH7660237.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(16165..16701)
                     /locus_tag="KW011_001898"
     CDS             complement(16165..16701)
                     /locus_tag="KW011_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HBH7660238.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            16848..17516
                     /gene="hxpB"
                     /locus_tag="KW011_001899"
     CDS             16848..17516
                     /gene="hxpB"
                     /locus_tag="KW011_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HBH7660239.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            17679..18269
                     /locus_tag="KW011_001900"
     CDS             17679..18269
                     /locus_tag="KW011_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBH7660240.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            18402..19793
                     /gene="tcyP"
                     /locus_tag="KW011_001901"
     CDS             18402..19793
                     /gene="tcyP"
                     /locus_tag="KW011_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HBH7660241.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(19797..20612)
                     /locus_tag="KW011_001902"
     CDS             complement(19797..20612)
                     /locus_tag="KW011_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660242.1"
                     /translation="MGREMFSTNLIQSNYGDLNIKSLAFDSFKERFESTMAALTFFIS
                     TGQCDCDEIAESNFNRMIAYMSNINYDASKPGAPALSFDTYLQDNVKYRVIINNLYGS
                     EFRIRDINENFVGMDFTCEFRPEKMTNLINIKNRLVIHYLRTIYYEKNYIHPAGSIFA
                     AIQKNESLLKFPSIISMLNINLLFNPLNLPGMGSGVLEDIMSIPDSSLRKRLGYEVLS
                     FSLQAHSLSQECIDKLDIFFADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
     gene            complement(20889..21152)
                     /gene="cedA"
                     /locus_tag="KW011_001903"
     CDS             complement(20889..21152)
                     /gene="cedA"
                     /locus_tag="KW011_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HBH7660243.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            21335..23596
                     /gene="katE"
                     /locus_tag="KW011_001904"
     CDS             21335..23596
                     /gene="katE"
                     /locus_tag="KW011_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HBH7660244.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(23854..24612)
                     /gene="chbG"
                     /locus_tag="KW011_001905"
     CDS             complement(23854..24612)
                     /gene="chbG"
                     /locus_tag="KW011_001905"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBH7660245.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(24625..25977)
                     /gene="celF"
                     /locus_tag="KW011_001906"
                     /gene_synonym="chbF"
     CDS             complement(24625..25977)
                     /gene="celF"
                     /locus_tag="KW011_001906"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBH7660246.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(26082..26924)
                     /gene="chbR"
                     /locus_tag="KW011_001907"
     CDS             complement(26082..26924)
                     /gene="chbR"
                     /locus_tag="KW011_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HBH7660247.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(26932..27282)
                     /gene="chbA"
                     /locus_tag="KW011_001908"
     CDS             complement(26932..27282)
                     /gene="chbA"
                     /locus_tag="KW011_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HBH7660248.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(27333..28691)
                     /gene="chbC"
                     /locus_tag="KW011_001909"
     CDS             complement(27333..28691)
                     /gene="chbC"
                     /locus_tag="KW011_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HBH7660249.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANAL
                     KF"
     gene            complement(28776..29096)
                     /gene="chbB"
                     /locus_tag="KW011_001910"
     CDS             complement(28776..29096)
                     /gene="chbB"
                     /locus_tag="KW011_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HBH7660250.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(29395..29733)
                     /gene="osmE"
                     /locus_tag="KW011_001911"
     CDS             complement(29395..29733)
                     /gene="osmE"
                     /locus_tag="KW011_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HBH7660251.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            29935..30762
                     /gene="nadE"
                     /locus_tag="KW011_001912"
     CDS             29935..30762
                     /gene="nadE"
                     /locus_tag="KW011_001912"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBH7660252.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREADIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            30992..31879
                     /gene="cho"
                     /locus_tag="KW011_001913"
     CDS             30992..31879
                     /gene="cho"
                     /locus_tag="KW011_001913"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HBH7660253.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(31839..32414)
                     /gene="ves"
                     /locus_tag="KW011_001914"
     CDS             complement(31839..32414)
                     /gene="ves"
                     /locus_tag="KW011_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HBH7660254.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT         8659 a         8095 c         7432 g         8462 t
ORIGIN      
        1 gtaagaatca tgctttgata accaattgat aactattatt atattaatct aacaccattg
       61 acatagttga ggtttgaatg aacaaaaagc tgtatttatt cccagactca aacatttttc
      121 tacaatgcaa ggacttgacg caggtaaatt tttccgagat tactgcttgt gatgaagtat
      181 gtatcattat tacaaggccc attcagcaag agattgaccg ccagaaaggg caaggtaatt
      241 cccgcctatc caaaaaagca aggaaggctg ctagcctatt taatcaagta gttgattcaa
      301 ctgacatgac tcttgttatt cgtgaacgct cgccgcgagt ttttcttacc atggatctaa
      361 gcctgaaacc atgtgaacag ttatctgaac aattggatta tcaggaggcc gacgatcgtt
      421 ttgttggtat tgcggcaggg tattgcgctg acgatccaga ttcggaggtg gcaatcatta
      481 ccaatgatag cggtccacgt ttttcagcaa taaaacataa tataatctgc tataaagtgc
      541 cttcttcatg gttgcttcct gctgagccag atgaaaagga taaacaaatt aaattgctgg
      601 aagctaagct gaagcaattc actcagtcta tgcccgattt cagcattaaa gtagccgatg
      661 acgagcaatt aaatattgta cttccttatt atacagcttt atctgaatat gaagttgata
      721 atttgctttg tcaactaacg gatgcattcc cattggttac aaactttgac aatgaacaaa
      781 ctaccccaca atcactagaa aaaatcatca agtcatttag tcaacaggag tatttccctg
      841 tatcagcaga tgatatttct ctttataaag aaaaacatta tccactttgg aaaaagcagt
      901 gtgaagaaat actgaaaaac tgtcatactc atattaatcc gaaggcaata aattacccgc
      961 tgtcgttttt attacaaaac acaggttata ctatcgctga acgtgctgtg gtaacattta
     1021 cagcaaaagg agattttcgt ctgtgtggat ttaattggga gataatggaa gagctgactg
     1081 aaagcaaaag gattgaccta ccttcagccc ctaccgcccc caaaggaaaa tggcgtagtg
     1141 tatgtggcgt aacagaaaga atgccttacg ctgggggagc tttggaaaca ccgctatcca
     1201 gtataatttc aaaagaaatt ggtcctttgt tatctcttac tccaacagaa agggataaaa
     1261 acgattttta ttataaagaa aagagaaatg agccagttca agttgtatcg ctggaatgcg
     1321 aagagtggcg tcatcaagtc aaagaagaaa tattcccgtt gttcgtatat acagccaaca
     1381 aaccaaagaa agtgaatggt gcaattgagg tgagagttga tgcaaataac cttaccaatc
     1441 cggtcattaa aacatttaaa ttaaaaataa ctatagaaga acatagtgca ttttcaacca
     1501 taacagaaat ggtgcagaca tacattcaag agcagaagaa aagacagaaa agttctatta
     1561 gcgcacaaat caaaacatga gggtgtactc aaataataca atgctatagt aaatctataa
     1621 gtgcataact atttcctgat cttttcccaa aatagatgac aggataaaaa acattccaat
     1681 cttttacagg ctttggataa atcgtatcaa agcctatttc ttcaagagtt aaatcaccgc
     1741 ttttcgctcg aaacggactg gctgttggag tatgagccag aacaacttag aatagcccct
     1801 gcactacgtt aagggaggtc gctatgttcc gttgtccgct ttgtggcgca tctgcccgta
     1861 tccgcaccag tcgtccggaa aatgattcaa acaccgtgcg gcaaaagtat taccagtgta
     1921 acaatctgga atgcggcgta tgcttctcaa cactggaagc tttccataaa ttcacatcaa
     1981 aacacgcctc cggcgttcac tcttcagaag atatcccgtg gcatgatctg ccagcttcac
     2041 acaggggaaa caatcagatg agcttgcctt tacctcagaa ttaacaggca gaattgccgg
     2101 agtaacaaaa aagcgataga ttacgcgcgg gtgcctttcg gctgatggtc ggagggaata
     2161 cccgaaggcc agatgtggaa aggccccgga aaacatctct gtttaaccga ggccctaacc
     2221 gcattacctt gacaagtgaa aggttagcgc ctctccggaa aaggagcaag tgctatgtcg
     2281 caaaaatcgc ttacgaccat cacgttctgc gtgacggcaa tcctcatcat ctggatgttg
     2341 cacggttcgc tgtgtgaaat acggatgagc ttctggggag cggagtttgc ggcgttctta
     2401 cagtgtaagc agtaaggaaa ccgcgacggg ggagcaatcc cccgtcaatc ggttgccagg
     2461 gtaaggccgg ataaggcacc ctatctcaca gatatgaaca acaaacccgc agcgtaaaaa
     2521 ctgcgggttt tctttttggt gccctcaccg acttgaaaca gtgataagac tataagacaa
     2581 agcccgcaat gtagcgggct tactcccctc accctccact gaatgtggac acacagtgga
     2641 acgaagcggc ataagatatt ataaatcaat cagttattga aagaaaaaag gccgcagagc
     2701 ggccttttta gttagatcag attactcgtc tttgggcgaa gcgttttcga cccggctttt
     2761 taacttctgc ccgggtctga aggtcaccac gcgccgtgct gtaatgggaa tatcctcgcc
     2821 cgttttcggg ttacgtcccg ggcgttgatt cttatcacgc agatcgaagt taccaaaacc
     2881 agagagtttc acctgttcgc cgttttccag agcgcgacgg atctcttcga aaaacagttc
     2941 aaccagttct ttggcatccc gcttgctaag cccaagctta tcaaacagat attctgacat
     3001 ttcagctttt gtaagcgcca taggttcaat ccctcaatga tgcctggaat cgctctttta
     3061 atgcctctac acatttggcg acggtagcgg caatctcctc ttcttcgagt gtacggctgg
     3121 tatcttgcag gatcaggctt atggcgaggc tcttataccc ctccgcaaca cccttaccgc
     3181 ggtacacgtc aaataagttt acgccaacta cctgatttac gccaactttc ttacattcgg
     3241 ataaaatatc cgctgcggga acgttttctg cgaccaccac cgcgatgtca cgacggttcg
     3301 ccgggaagcg agaaatctcg cgcgcctgag gcaccacgcg gtctgcgagc ttgttccact
     3361 ccagttcgaa caccagagtg cgaccgttaa gatccagttt acgttccagt tcaggatgaa
     3421 caactccaac aaaaccaata cgttcccctt tcagataaat cgctgcggat tgccccggat
     3481 gcagtgccgg attcgcttct gcacggaact caacctcatt cagtttaccg gtcaggtcga
     3541 gaacggattc aagatcgcct ttcaaatcat agaaatcaac ggtctctttt gccaggttcc
     3601 agtgctcttc gtaacggtta ccgcaaatca caccggctaa catcagatcc tgacgaatgc
     3661 ccaacggtgc ctgagtatct ggtacgaaac gcagaccgct ttcgaaaatg cgcacacggt
     3721 tctgctgacg gttctggttg tacaccacgg ttgccagcag gccggtccac agagaaagac
     3781 gcattgctga catttcaaca gagatcgggc ttggcagcag taaggcttca acgcctggat
     3841 ggatcatctg ctgcactttc ggatcaacga agctgtaggt gatcacttcc tgatagcctt
     3901 tgtcgttgag cagcgttttc acgcgcttga gcgacaggtc agcttcacgg tgagtaccca
     3961 taatcaggct tgcctgtacc ggctcatccg ggatgttgtt gtagccgtaa acacgcgcga
     4021 cttcttcaac cagatcttct tcaatctcca tatcgaaacg ccagctcggc gcaactgcct
     4081 gccactcgtc tttgccttcg gtcacttcgc agccgagacg acgcagaatg tcagttacct
     4141 gttcatcagc aatatgatgg ccgatcaggc gatccagttt gctacgacgt aaagtgatgg
     4201 ttgcacgctt cggcagcgtt gcttcgttgg tgatatcaat taccggacca gcctcaccac
     4261 cgcagatgtc gatcagcaga cgggtcgcac gttccatcgc tttgtgctgc agtgccggat
     4321 caacgccacg ctcataacgg tgagacgcat cagtatgcag gccatgacga cgagcacgac
     4381 cggtgataga cagcgggctg aagaaagcgc attccagcaa gacgttttgc gtttcgtcat
     4441 tcacgccaga gtgttcgcca ccaaagatgc cgcccatcgc cagcgccttg ttgtggtcgg
     4501 cgatgaccag agtgtcagca ttcagcttcg cttcagtgcc atcaagcagc accagcgttt
     4561 cgccctcttt tgccatccgc accacaatgc cgccttcaat gcgatcttta tcgaaagcgt
     4621 gcatcggctg gcccagttcg agcagtacat agttggtgac gtcaacaact gcatcgatag
     4681 aacggatccc gcaacgacgc agtttttctt tcatccacag cggagttggc gctttaacgt
     4741 taatgccttt taccacacgg ccaagatagc gcgggcaggc ttcaggcgct tcgactgtaa
     4801 tcggcaacgt gtcgtcgatg gtcgcaccaa ccggaacgat ttccggttca accagcggca
     4861 gctgattcag cacggcaacg tcacgcgcaa caccaatgat acctaagcag tcggcacggt
     4921 ttggcgttac gctgatttcg atggtgttgt catcgagttt caggtattcg cggatgtcag
     4981 tgccaatcgg cgcatctgca ggcagttcga taatgccgct gtgatcgtca gaaattccca
     5041 gttcagagaa ggagcacagc atcccttcag acggttcgcc acgcagtttc gccgctttaa
     5101 ttttgaaatc acccggcaga acagcaccaa tggtagctac cgccacacgc aggccctgac
     5161 ggcagtttgg cgcaccacag acgatgtcca gcaggcgatc gccgccgaca ttcacttttg
     5221 tcacacgcag tttgtcagcg ttcggatgct gcgcacactc aaccacttca ccaacgacca
     5281 cgccgtggaa gctaccggca accggttcta caccgtcaac ttccaggccc gccatagtga
     5341 tttgattcgc cagcgcatcg ctatcaatcg ccgggttcac ccattcgcgt aaccacagtt
     5401 cactgaattt cataatctat tcctgcctta tttaaactgt ttgaggaaac gcagatcgtt
     5461 ttcgaagaat gaacgcaggt cggtgacgcc gtaacgcaac atagtcagac gctccatccc
     5521 catcccgaag gcgaaaccag agtaaacttc cgggtcgatg ccaacgttac gcagcacgtt
     5581 cggatgcacc atcccgcaac ccagcacttc cagccattta ccgtttttac ccatgacgtc
     5641 aacttctgca gaaggctcgg tgaacgggaa gtaggaagga cggaagcgaa tctgcaaatc
     5701 ttcctcaaag aagttacgca ggaaatcgtg cagagtgcct ttcaggttgg taaagctgat
     5761 gttggtatca acaatcagac cttccatctg atggaacatc ggcgtgtgag tctggtcgta
     5821 gtcgttacga taaacgcggc caggcgcgat gatacgaatc ggtggctgct gggctttcat
     5881 ggtgcggatc tgtacgccag aggtctgggt acgcagcagg cgggtagtgt caaaccagaa
     5941 agtgtcgtgg tcagcgcgcg ccgggtggtg accaggaatg ttcagagcat cgaagttatg
     6001 ataatcgtct tcgatttccg gcccggttgc cacggtaaag ccaagctcac cgaagaaact
     6061 ttcgatacgg tcgatggtac gggtaaccgg atgcagaccg ccgttttcaa tgcgacgacc
     6121 tagcagggag acatcaatcg tttccgccgc cagacgcgca ttcagcgctg cgctttccag
     6181 ttccgcttta cgcgcattca gcgcctgctg aacctgctct ttcgcttcgt tgataaccgc
     6241 accagctgcc ggacgctctt ctggcggcag ctcacgcagg gtcgtcatct gaagggttaa
     6301 gtgccctttt ttacccaaat attcgacgcg cacattatct aacgcggcaa catctgacgc
     6361 ctggctaatg gccgccttcg cactggcaac cagttctgcg agatgtgaca tggttttcct
     6421 cattgtgtca gtggtgacac tggttcgttg gacttagagc ctatcccatc aggctatttt
     6481 acttgccatt ttggtcccgg gcagtgctcg aaattctcac gtacttaaat acgctccggt
     6541 ttctccgcgc tggccgtgtc cagactggct gcgacaatta cgcctgatag actggctttt
     6601 tatttttcaa aacgcgcata caaaaaaagc ctccactggg aggctttcag gcgctgtttt
     6661 ccgtttctct tctcacgcgc tagcctcctg gattcaggtg ctaaagtaaa aaaagaagcg
     6721 gaaaatagca gcattcattg cttgcgttac cttttggtac tcttcaaaag acctttattg
     6781 aaaaggctac ggcgataaaa gtcaatgttt tgatggcgtt gaaacgaaaa gagggagact
     6841 agctccctct ttcaactggc ttatgccaga gctgctttcg ctttttcaac cagagcggtg
     6901 aacgctactt tgtcgaatac tgcgatatca gccaggatct tacggtcgat ttcaacagag
     6961 gcttttttca ggccattgat gaatttgctg taagaaatac cgttctgacg tgctgctgcg
     7021 ttgatacgcg caatccacag ttgacggaac tgacgcttac gttgacgacg gtcacggtaa
     7081 gcatactgac cagctttgat aacagcctgg aaggcaacgc ggtatacgcg agaacgcgca
     7141 ccgtagtagc ctttagcttg tttcaaaatt ttcttgtgac gtgcgcgtgc aataacacca
     7201 cgttttacgc gagccatatg tgctctcctg tatctatatt ctaattaaaa agttaaaaac
     7261 gttaacggct tatgcgtacg gcaggcacgc gattaccagg cccagatcgc ctttggaaac
     7321 catggctttc ggacgcaggt gacgtttacg tttggtcgct tttttggtca gaatgtgacg
     7381 caggttagcg tgcttgtgct taaaaccacc tttaccggtt tttttgaagc gcttagcagc
     7441 accgcgtacg gtcttaattt ttggcatttt aataacttcc acttcgcatt gttaataaac
     7501 gaaacaaagg cgaacaaagc ctgtgaagcc cgaaggctcc acagacagtg ctacttgaag
     7561 gccttactgt ttcttcttag gagcgagcac catgatcatc tggcggcctt cgatcttcgt
     7621 tgggaaggat tcgaccactg ccagttcttg caaatcgtct ttcacgcgat taagcacttc
     7681 cataccgatt tgctggtgcg ccatctcacg accgcggaaa cgcagcgtga ttttggcttt
     7741 atcaccctct tcgagaaagc gaatcaggct gcggagtttt acctgatagt cgccttcatc
     7801 tgtaccagga cggaatttaa tttccttaac ctggataact ttttgctttt tcttctgttc
     7861 cttagaagac ttgctctttt catagaggaa tttgccgtaa tccattatac gacaaaccgg
     7921 cggctcggcg ttagggctga tctcgactaa gtctactccg gcttcttctg ctttctccag
     7981 agcttctctc agactcacaa taccaagctg ctcgccttcc agacctgtta agcgaacttc
     8041 ctgggcgcga atttcgccat tgatacggtt agggcgcgcc gtttgaactc gttttccgcc
     8101 tttaatacct tattcctcca attgtttaag actgcggctg cgaatctctt gttgcagctt
     8161 ctcgatcact tcatttacgt ccatgcttcc caggtcttta ccacggcggg tgcgaacggc
     8221 aactttgcct gattccacct ctttatcacc acagaccagc atatatggga cgcgacgcaa
     8281 agtgtgctcg cggattttaa agccaatctt ctcatttctc aagtctgctt taacacgaat
     8341 gcccgcattt gatagttttt gcgtcaattc gttaacgtat tcagactgtg aatcggtaat
     8401 attcatgata acaacctgaa ctggcgcaag ccaggtcggg aagaaaccag cgaattcttc
     8461 ggtcaggata ccgatgaaac gttccatcga ccccagaatt gcgcggtgaa tcattaccgg
     8521 taccttacgc tcgttgtctt cgcccacata ggaggcgctc agacgagacg gcaaggagaa
     8581 gtccagctgt actgtaccgc actgccatgc acgatcgagg cagtcataca gggtaaattc
     8641 aattttcgga ccgtagaaag cgccttcacc cagttgatat tcaaacggga tgttgttttc
     8701 ttccagcgca accgccaggt ccgcctcagc acgatcccac atttcgtcgc tgccaatacg
     8761 tttttcagga cgagtggaga gtttgacgac gatcttctcg aagccaaaag tgctgtacat
     8821 atcatagact aaacggatac atccgttaac ttcatcgcga atttgttctt cagtacagaa
     8881 gatatgcgcg tcatcctggg taaatccacg cacgcgcatc aggccatgca gcgaacctga
     8941 cggctcgtta cggtggcagc taccaaactc ggccatacgc agcggcagat cgcgataaga
     9001 cttcagcccc tggttgaaaa tttgtacgtg acccgggcag ttcatcggct taatgcagta
     9061 ttcacggttc tcagaagatg tggtgaacat tgcatctttg tagttgtccc agtgaccggt
     9121 tttttcccac aggacacggt ccatcatgaa cggaccttta acttcctgat actggtactc
     9181 tttcagttta gaacgaacaa acacttccag ttcacggaag atggtccagc cgtcgttgtg
     9241 ccagaatacc atacccggcg cttcttcctg catatggtac aggtcgagct gtttaccgat
     9301 tttacggtgg tcgcgtttcg cggcttcttc caggcgctgc aggtaagcgt taagtgcttt
     9361 tttgtctgcc cacgccgtac cgtaaatacg ttgcaacatt ttgttgttgc tgtcgccacg
     9421 ccagtaagcc cctgccgttt tcattagttt gaaatgatgg cagaaacgca tgttcggtac
     9481 gtgcggaccg cggcacatat cgacatattc ttcatggaag tacagacctg gcttgtcatc
     9541 atgggcgatg ttttcgtcaa gaatggagac tttgtagctc tccccacggt tggcgaaagt
     9601 ttcacgcgct tcgtgccagc tgactttctt cttaatgacg tcgtagtttt tctcagcaag
     9661 ctcatgcatc cgcttctcga gtgcttcgac atcttcctgg gttaacgtgc ggtcaagatc
     9721 aacgtcgtaa taaaaaccgt tgtcaataac cgggccgatt gccattttgg tatgcggcca
     9781 aagttgttta atcgcatgcc ctaacaggtg cgcacaggag tgacgaatga tctccagacc
     9841 ttcttcgtct ttggcggtaa tgatcgacag ttgtgcgtcg ttttcaatca gatcgcaagc
     9901 atcaaccagt tcgccattaa cgcgccctgc gatacaggct ttcgccagac ctggaccaat
     9961 gtccagcgca acatccatgg ggcttacagc gtggtcgtaa tggcgttggc tgccatcagg
    10021 aagagttata acaggcattt tatatcctta atttgcagtg gtgacccaca cgaaagatca
    10081 catacaaaga aaaatttgtt tattaacagt tcattgcgaa accatctagc caacaaatgc
    10141 taaattggtt cgcaatcagg tacacaaatg aaagacatta acctctcacc ttccacccgg
    10201 ttgaacgatg ttaacactaa aaaaagggag attgtacctt tccgtttcac attgattcga
    10261 ttcgcaattc gtttgtagtg agtacaccag caaaacgctt aaagaaccac tgataacatc
    10321 acggtgcgtc tgaaaggttt actgcgagcg gatttacagc agcataaact gaatccagca
    10381 aagttcgttg cgtgatttgg cggtagtatt ttaaacgctc atgagattaa tgacgaagtg
    10441 gtcatatcac aatgataaaa gtgacacaat tcttatagca atttttcgtg cacatttcgt
    10501 tctggcgata ataattaatc atcatcctca aaacctccag acatatataa ggcgaataga
    10561 ttatgagtca aaacgatatc attatcagaa cccattataa gtctcctcat agattgcaca
    10621 tcgatagcga cataccaacg ccttcatcag agcctattaa tcaatttgcg cgccaactca
    10681 tcaccctact tgatacctct gacttaagtt cgatgctgtc atactgtgtt actcaggaat
    10741 ttaccgcaaa ctgtcgaaaa atttcacaaa attgttattc cactgccctt tttatcatta
    10801 actttgccac ttcacccatc catgcagaaa atatactcat tacattacac tataaaaaag
    10861 aaatcattcc cttatttctg gaaaccacgc ctattaaggc taaccatttg cgaagcatac
    10921 tggattatat tgaacaggaa cagttaactg ccgaagatcg taaccattgt atgaaactgt
    10981 ctaaaaaaat ccatagagaa aaaactatac aaccaacagt aaatctcaat ggtagtgcat
    11041 ttttttcgca atctccttct gacgctattt tttgtcgcca tctgtcattg caatacgccc
    11101 ttgattcatt gagaaatgga aaaggcaaag tcaacctgat taaacactac tcctccgttg
    11161 aatccataca gcagcatgtc cccttagtcc gggacgcgga gttcagagca ttacttcgcc
    11221 atcctcctgc agggagtcgc gttatcgcga gtaaggattt tggcttcgct ttagatattt
    11281 tcttttgtcg aatgatggca aacaatgtca gtcatatgtc cgcgatttta tatatagaca
    11341 atcatacttt gtcagtaagg ctacgaataa agcagtcagt gtatggacaa ttaaattatg
    11401 ttgtgtctgt ttacgacccg aacgatacca acgttgccgt cagaggcacc cacagggcag
    11461 cacggggctt tctctcgctt gataagttca tcagttcagc tcccgatgct cagacctggg
    11521 ctgataggta tgttcgcaac tgtgcaattg ctattcttcc tctattacct gagggagttc
    11581 cagttgctat tttagcgggt atcacgacac gaatgccatt tgcccctata catccatcgg
    11641 caatgttgtt aataatggcc acaggccaga ctcaatagct tattacacta ttcaaacagt
    11701 tgcccatact ccctgaaaaa gaaatcattg aaataataac tgcgcagaat agcgttggta
    11761 cacctgcttt atttctggct atgatgaacg gacaaactga caacgtaaaa atatttatgc
    11821 aagaaattca gtcactggta tacaatcata tcattcatga agataatctg gttaaattac
    11881 tgcaaactaa aagtgctaac gaaacacctg gactctatat ctccatgttg tatggattcg
    11941 atgaaataat cgatatcttt ctgaatgcat taaccactcc aatagcacaa gaacttttaa
    12001 acaaaaaaat ggtgatgagt attttagcca tgaaaataca tgatggtgag ccaggattat
    12061 acgccgcaat ggaaaataat caccctttgt gtgtcacacg gttcctctct aaaattaatg
    12121 gcatcgcctt taaatacaag ttgagcaaag ctaacatcat ggatttatta aaaggagcta
    12181 cagcacaggg aacccctgca ttatacatcg ccatgagcaa gggtaatgaa gacgtcgtgt
    12241 tatcttatat atcgacgctg ggtgcttttg caaaaaaaca ttcttttagt caacatcagt
    12301 tatttacact attggccgct aaaaatcatg acaacatgtc agctgttcat atagccattc
    12361 atcataagca ttataaaact gtagaaacat attatgctgc tataaatgtc atcagccaaa
    12421 gcctgagttt tagtgctgat gaaataaaga cgtatttata accagctatt tacgatatat
    12481 atcgatacat aattttattt ttttcataaa acgcagaaac agtgaccatg tgcacaacgc
    12541 cacccgacgg ctgtaataac aaaaaatccc ttgatgccta tcccttttgt tacactccgt
    12601 tatcacgcac aagagatatg caggacactg gtatgccgac taaacgcttt gataaaaaac
    12661 actggaagat ggtggtggtg ctactggcaa tctgtggcgc tatgttgttg ctacgttggg
    12721 cagcaatgat ttggggctga gcagtgacaa acgaagacag ccagaccgca taacacggtc
    12781 cggcagataa ttttacatgc tgtagcccag cgataaggtt gtccgacgat cggtatgttc
    12841 cggtgcagac tctggaggtt cagagttcca tgtgacgttg taagccacct tcagtccaaa
    12901 atgttcatta atagcaacat ttaatgcgct ttcggagttc agcgttgtat cttccgcgcc
    12961 aaagaccgaa acaccctgcg taaatttagc gttgtcagtc aactgccatg cataagcccc
    13021 ggaggcatag cccagcggct gggtttcact ggcattgtcg gtatatttgt cgtaacgcac
    13081 acctgggccg aattcaaagc ggaaactgtg taccgggcca ttcaaaaact ggcgaccata
    13141 acccgcggtc aacacatcgc gctctcgata gccgttataa cggtctgtca gccagcttgc
    13201 ctgaccaaat aaatagtcat aatcagttaa attaaaacgg ctacgcccgc ccgccgcata
    13261 tttttctgaa gaacgctcat cattagaaga agtattactg gcgttccccc acagcgacca
    13321 ggcagtggtt tgtccatacc aggtcatggt ggtgtcagcc gtaagtgagg agcttttcgt
    13381 attgcctgat tgtgcaagat atcctgcgtt cagattacct tcgaaaggtt ttttagcgct
    13441 ggcaggatcg tccatgacag taaaaacgga atcatcggca gctgcattca gtgacgcaaa
    13501 catgcccccc gccaacataa cgatggcagg aactgtcttc aaaagcttca tttatcaaga
    13561 gtccgtacaa caaaaaaaga gaccatcgcg gtcccggaaa ctttcttaag gatcaaagat
    13621 tagcgtccct ggaaaggtaa cgaattataa aaaggcgcga ataacttagc aatgtattct
    13681 tatttcattt tttgaataag catgtggcga aaacagattt ttatttatat atatttatct
    13741 gcaaaatttt aaataaagct ccaataaatc atattgttaa tttcttcact ttccgctgat
    13801 tcggtgccag actgaaatca gcctatagga ggaaatgatg gtacgtatct atacgttgac
    13861 acttgcgccc tctctcgata gcgcaacaat taccccgcaa atttatcccg aaggaaaact
    13921 gcgctgtacc gcaccggtgt tcgaacccgg gggcggcggc atcaacgtcg cccgcgccat
    13981 tgcccatctt ggaggcagtg ccacagcgat cttcccggcg ggtggcgcga ccggcgaaca
    14041 cctggtttca ctgttggcgg atgaaaatgt ccccgtcgct actgtagaag ccaaagactg
    14101 gacccggcag aatttacatg tacatgtgga agcaagcggt gagcagtatc gttttgttat
    14161 gccaggcgcg gcattaaatg aagatgagtt tcgccagctt gaagagcaag ttctggaaat
    14221 tgaatccggg gccatcctgg tcataagcgg aagcctgccg ccaggtgtga agctggaaaa
    14281 attaacccaa ctgatttccg cttcgcaaaa acaagggatc cgctgcatcg tcgacagttc
    14341 tggcgaagcg ttaagtgcag cactggcaat tggtaacatc gagttggtta agcctaacca
    14401 aaaagaactc agtgcgctgg tgaatcgcga actcacccag ccggacgatg tccgcaaagc
    14461 cgcgcaggaa atcgttaata gcggcaaggc caaacgggtt gtcgtttccc ttggtccaca
    14521 aggagcgctg ggtgttgata gtgaaaactg cattcaggtg gtgccaccac cggtgaaaag
    14581 ccagagtacc gttggcgctg gtgacagcat ggtcggcgcg atgacactga aactggcaga
    14641 aaatgcctct cttgaagaga tggtccgttt tggcgtagct gcggggagtg cagccacact
    14701 caatcaggga acacgtctgt gctcccatga cgatacgcaa aaaatttacg cttacctttc
    14761 ccgctaacaa aaacattccc ccagcattgg gggaatcacc accaacctgt cggcaacgcg
    14821 tttctccgac tttgctcaaa agtcatgtga taacaaaggg gtgaactatg gccagtggcg
    14881 atcttgtccg ttatgtcata accgtcatgt tgcatgagga tacattgact gaaattaacg
    14941 agttgaataa ttacctgact cgcgacggtt ttttgctcac catgacggat gatgagggaa
    15001 atatccatga gctggggact aacacttttg gacttatcag tacccaaagt gaagaagaaa
    15061 ttagagaact ggtttcgggg cttacccaaa gtgcaaccgg caaagatcct gaaatcacca
    15121 tcacgacttg ggaggaatgg aatagcaaca gaaaataaat ggtttttggg caataatcag
    15181 tctgtggtgt gcgttagctc gtgtttttac accgcattct tgcgctaacc ttatgatctg
    15241 gcagacaaca tgggagagac atcatgtggc aggcaatcag tcgtcttttg agcgagcagt
    15301 taggtgaagg cgaaatcgaa ctgcgtaatg aactgcctgg cggagaagtc catgccgcat
    15361 ggcatttgcg ctatgcagga catgactttt tcgtcaaatg tgatgaaagg gaactgcttc
    15421 ccggttttac cgccgaagcc gaccaactgg agttactgtc gcgtagtaaa accgtcaccg
    15481 tgcctaaggt ttgggcagta ggcgctgacc gtgactacag ttttctggtg atggattatc
    15541 tcccacctcg tccgctggat gcgcatagcg catttattct tggtcagcaa attgcgcgtt
    15601 tacatcaatg gagtgaccaa ccacaatttg gcctcgattt tgataactcg ctctccacaa
    15661 ctccacagcc caacacctgg caacgtcgct ggtcaacgtt ttttgctgaa caacggattg
    15721 gctggcagtt ggaactggca gcagagaaag ggatcgcttt cggcaatatc gacgccatcg
    15781 tcgagcatat tcagcagcgt ctggcctcgc atcaactgca gccttctctg ttgcacggcg
    15841 atttatggtc cggcaactgt gcgctgggtc cggatggccc gtacattttc gaccctgcct
    15901 gctactgggg tgaccgagag tgcgacctgg cgatgttacc gctgcatact gaacagccgc
    15961 cacaaatcta tgacggctat cagtcagtat ccccgctacc tgccgatttc cttgaacgtc
    16021 aaccggttta ccaactctac acgctgttaa atcgtgcaag attatttggc ggtcagcatt
    16081 tggttattgc tcagcagtca ttggatagat tattagcagc atgatatggg ttgaggataa
    16141 tggccgctcc gtgcggcctt ttgattaaat aaacccaagc agagaaaaga acacataacc
    16201 tgcagcaata caaataaccg gcaggatata tagtgagaaa aactgcagga aaatagtatg
    16261 gtgcggaaca acaatacggg actcaatttg ttcacgcgtt agcccctctt cccctttggc
    16321 tttttccaga atgagttgat cttcaacacc ttcccgcaag aagcgcgcct ggcgactcat
    16381 ccgggcaccg gaatcctgca acgccagtcc gacaaaaatc aggatgaaaa tcacccagaa
    16441 cataacgttc acgccaccat taaaattagg ggtcggggag ttataccaga acagattcaa
    16501 aaaaggcgta ttcgcctgca tcatatcgat catgacatga gtaaagtcga gcatgaccgc
    16561 attaatgcct tcctggtttt cctgccgggt attcataaat ttcagcaaag aaatcaatgt
    16621 ggagatcaat gcaggtataa aaatcaccca acccaaaatc cttttcaaaa cagcaatgcg
    16681 tccagcttgt tgatacgtca tgagttctcc ttgattaaga cgcgtcgttt cacttagttt
    16741 acctgtagat atctgttttc gcccattctt taaaggcgat atgataggcg cttaatcata
    16801 agcacggctt aataccttac acataatgct ctaaaggaga ggttgtaatg tcaaccccgc
    16861 gtcagattct tgctgcaatt tttgatatgg atggattact tatcgactca gaacctttat
    16921 gggatcgagc cgaactggat gtgatggcaa gcctgggggt ggatatctcc cgtcgtaacg
    16981 agctgccgga caccttaggt ttacgcatcg atatggtggt cgatctttgg tacgcccggc
    17041 aaccgtggaa tgggccaagc cgtcaggaag tagtagaacg ggttattgcc cgtgccattt
    17101 cactggttga agagacacgt ccattattac caggcgtgcg cgaagctgtt gcgttatgca
    17161 aagaacaagg tttattggtg ggactggcct ccgcgtcacc actacatatg ctggaaaaag
    17221 tgttgaccat gtttgactta cgcgacagtt tcgatgccct cgcctcggct gaaaaactgc
    17281 cttacagcaa gccgcatccg caagtatatc tcgactgcgc agcaaaactg ggcgttgacc
    17341 cactgacctg cgtggcgctg gaagactcgg taaatggcat gatcgcttct aaagcagccc
    17401 gcatgcgttc catcgtcgtt cctgcgccag aagcgcaaaa tgatccacgt tttgtattag
    17461 caaacgtcaa actctcatcg ctgacagaac tcaccgcaaa agaccttctc ggttaatgac
    17521 caggggcagt gatcacgctg cccctggttc tttatctgaa tttgcattca actgacggat
    17581 taatcgtcaa tttaagagaa agagttacac cgtcaccact tccgtgcact gtataaaaat
    17641 cctatactgt acgtatcgac agtttagtga gttttatcat gacggcggaa ggtcaccttc
    17701 tcttttctat tgcttgtgcg gtatttgcca aaaatgccga gctgacgccc gtgctggcac
    17761 agggtgactg gtggcatatt gtcccttccg caatcctgac gtgtttgtta ccggacatcg
    17821 atcacccaaa gtcgtttctt gggcagcgat taaaatggat atcaaaaccg atcgcccgcg
    17881 cttttgggca tcgtggtttt acccacagtc tgctggcggt atttgcgctg ctggcaacct
    17941 tttaccttaa ggttccggaa ggctggttca ttccggctga tgcgctacaa ggaatggtgc
    18001 tgggttattt gagccacata cttgccgata tgctgacacc cgccggtgtt cccctgctct
    18061 ggccatgccg ctggcgtttc cgcttgccta tcctggttcc ccaaaagggc aaccaactgg
    18121 aacgttttat ctgcatggca ttatttgtct ggtcggtatg gatgccccat tcattacccg
    18181 agaacagcgc tgttcgttgg tcatcgcaaa tgatcaatac cttgcagatc cagtttcatc
    18241 ggcttattaa gcatcaggtt gaatactaaa aaggcaaaaa tcacctttct ggaataagca
    18301 attccatttg aatataagag ccagctcaca gttctgttaa tcttgcgcca acactatgac
    18361 tgctacgcag tgatagaaat aataagatca ggagaacggg gatgaacttt ccattaattg
    18421 cgaacatcgt ggtgttcgtt gtactgctgt ttgcgctggc tcagacccgc cataaacagt
    18481 ggagtctggc gaaaaaagtg ctggtgggtc tggtgatggg tgtggttttt ggccttgccc
    18541 tgcataccat ttatggttct gacagccagg tacttaaaga ttctgtacag tggtttaaca
    18601 tcgttggtaa cggctatgtt caactgctgc aaatgatcgt tatgccgtta gtcttcgcct
    18661 ctattctgag cgcggttgcc cgtctgcata acgcatctca gttaggcaaa atcagttttc
    18721 tgaccatcgg tacgcttttg tttaccacgc tgattgcggc gctggtcggt gtgctggtca
    18781 ccaacctgtt tggtttgacg gctgaaggtc tggttcaggg tggtgcagaa actgcacgtc
    18841 tgaacgccat tgaaagtaac tatgttggta aagtctctga tctgagcgtt ccgcagctgg
    18901 tcttgtcctt tatcccgaaa aacccgtttg ccgatcttac cggagccaat ccgacgtcaa
    18961 ttatcagcgt ggtaattttt gccgcattcc tcggcgtagc tgcattgaaa ctgctgaagg
    19021 atgatgcgcc gaaaggtgaa cgcgtcttag ccgctatcga taccctacaa agctgggtga
    19081 tgaaactggt tcgccttgtc atgcagttga ccccttacgg cgttctggct ctaatgacca
    19141 aagtggttgc aggttctaac ttgcaagaca tcatcaaact gggaagtttc gttgtcgcgt
    19201 cctacctcgg tctgctgatt atgtttgcag tgcatggcat tctgctgggc attaatggcg
    19261 tgagtccgct gaagtacttc cgtaaggtat ggcctgtgct gacgtttgcc tttaccagcc
    19321 gttccagtgc tgcgtctatc ccactgaatg tggaagcaca aacgcgtcgt ctgggcgttc
    19381 ctgaatccat cgccagtttc gccgcctctt tcggtgcaac cattggtcag aacggctgcg
    19441 ccggtttgta tccggcaatg ctggcggtga tggttgcgcc tacggttggc attaacccgc
    19501 tggacccgat gtggattgcg acgctggtcg gtattgttac cgttagttcc gcaggcgttg
    19561 ccggtgtcgg tggtggtgca actttcgccg cactgattgt gctgcctgcg atgggcctgc
    19621 cagtaaccct ggtggcgctg ttaatctccg ttgaaccgct tatcgacatg ggccgtacgg
    19681 cgctaaacgt tagtggctcg atgacagctg gcacgctgac cagccagtgg ctgaagcaaa
    19741 ccgataaagc cattctggat agcgaagacg acgccgaact ggcacaccgt taattcttat
    19801 gctggcaacg gtccgttttg tataggggcc gttgctttac tttttaaatg ttccatcgcg
    19861 gcgatgcata ctgactcata tttaaacaga tcgtcagcga aaaaaatgtc caacttatca
    19921 atacattcct ggctcaatga atgtgcctgt agtgagaagc ttaaaacctc atacccgaga
    19981 cgttttctaa gtgaactatc tggaatactc ataatatctt caagcacacc acttcccatt
    20041 cccggaagat ttagtggatt aaaaagaaga ttgatattaa gcattgatat tatcgaagga
    20101 aactttagga gactctcatt tttttgaatc gcagcaaaaa tgctcccggc aggatgtata
    20161 taattttttt catagtatat agtccttaaa taatgaataa ccaacctatt ttttatattg
    20221 atcaaattcg tcattttttc gggtcggaat tcacaggtaa aatccatccc aacaaaattc
    20281 tcattaatat ctcttatccg aaactcactg ccataaagat tattaattat cactctatat
    20341 ttaacattgt cctgcagata cgtatcaaaa ctaagtgctg gagctcctgg ctttgaggca
    20401 tcatagttaa tgttagacat ataggcaatc atacgattga agttcgactc tgctatttca
    20461 tcgcagtcac attgacccgt tgatataaag aaagtgagtg ccgccatggt agactcaaac
    20521 ctttctttaa aagagtcgaa agccaggctt ttaatattta aatcaccata attactctgt
    20581 attaagtttg tagaaaacat ctcccgcccc atattgttaa caaaattatt atctcattta
    20641 aatctaagtc acttacaata taagtttaag agcgacgcca caggatgaac tatcaaaaat
    20701 agctcatcat gattagcaaa acttaaccat tttaaaataa ataaacaatt aaagaaaaaa
    20761 gatcacttat ttatagcaat agctcgtcaa aggcagcttt ttgttacagg tggtttgaat
    20821 gaatgtagca acgaaatgca gaatttcagg tcatgtaact cccggcaaaa ccgggaagta
    20881 tgtaatcctt actcagtcac ttccccttcc tggcggatct gatttgccca acgttgggca
    20941 gattcaggca cagtaaacgc cggtgagcgc agaaatgact ctcccatcag tacaaacgca
    21001 acatatttgc cacgcagcat ccagacatca cgaaacgaat ccatctttat cgcatgttct
    21061 ggcggcgcgg gttccgtgcg tgggacatag ctaataatct ggcggttttg ctggcggagc
    21121 ggtttcttca ttactggctt cactaaacgc atattaaaaa ttagaaaaac tgtagtttag
    21181 ccgatttagc ccctgtacgt cccgctttgc gtgtatttca taacaccgtt tccagaatag
    21241 tctccgaagc gggatctggc tggtggtcta tagttagaga gttttttgac caaaacagcg
    21301 gccctttcag taataaatta aggagacgag ttcaatgtcg caacataacg aaaagaacct
    21361 acatcagcac cagtcaccac tacacgattc cagcgaagcg aaaccgggga tggactcact
    21421 ggcacctgag gacggatctc atcgtccagc ggctgaacca acaccgccag gtgcacaacc
    21481 taccgcccct gggagcctga aagcccctga tacacgtaac gaaaaactta attctctgga
    21541 agacgtacgc aaaggcagtg aaaattatgc gctgaccact aatcagggcg tgcgcatcgc
    21601 cgatgatcaa aactcactgc gtgccggtaa ccgtggtcca acgctgctgg aagattttat
    21661 tctgcgcgag aaaatcaccc actttgacca tgagcgcatc ccggaacgta ttgttcatgc
    21721 acgcggatca gcagctcacg gttatttcca gccatataaa agcttaagcg atatcaccaa
    21781 agcggatttc ctctcagatc cgaacaaaat caccccagta tttgttaggt tctctaccgt
    21841 tcagggtggc gctggctctg cagataccgt gcgtgatatc cgtggttttg ccaccaagtt
    21901 ctatactgaa gagggtattt ttgacctcgt tggcaataac acgccaatct tctttatcca
    21961 ggatgcgcat aaattccccg attttgttca tgcggtaaaa ccagaaccgc actgggcaat
    22021 tccacaaggg caaagtgccc acgatacttt ctgggattat gtttctctgc aacctgaaac
    22081 tctgcacaac gtgatgtggg cgatgtcgga tcgcggcatc ccccgcagtt accgcaccat
    22141 ggaaggcttc ggtattcaca ccttccgcct gattaatgcc gaagggaagg caacgtttgt
    22201 acgtttccac tggaaaccac tggcaggtaa agcctcactc gtttgggatg aagcacaaaa
    22261 actcaccgga cgtgacccgg acttccaccg ccgcgagttg tgggaagcca ttgaagcagg
    22321 cgattttccg gaatacgaac tgggcttcca gttgattcct gaagaagatg aattcaagtt
    22381 cgacttcgat cttctcgatc caaccaaact gatcccggaa gaactggtgc ccgttcagcg
    22441 tgtcggcaaa atggtgctca atcgcaatcc ggataacttc tttgccgaaa acgaacaagc
    22501 agcattccat ccaggtcata ttgttcctgg tctggacttc accaacgatc cgctgttgca
    22561 gggacgtttg ttctcctata ccgatacaca aatcagtcgt cttggtgggc cgaatttcca
    22621 tgagattccg attaaccgtc cgacctgccc ttaccacaac ttccagcgtg atggcatgca
    22681 tcgtatgggg atcgacacta acccggcgaa ttacgaaccg aactcgatca acgataactg
    22741 gccgcgcgaa acaccgccgg gaccgaaacg cggcggtttt gaatcatatc aggaacgcgt
    22801 ggaaggaaat aaagttcgtg agcgcagtcc atcgtttggc gaatattatt cccatccgcg
    22861 tctgttctgg ctaagtcaga cgccattcga gcagcgccat attgtcgatg gtttcagttt
    22921 tgagttaagc aaagtcgttc gtccgtatat tcgtgagcgc gttgttgacc agctggcgca
    22981 tattgatctc actctggccc aggcggtggc gaaaaatctc ggtatcgaac tgactgacga
    23041 ccagctgaat atcaccccac ctccggacgt caacggtctg aaaaaggatc catccttaag
    23101 tttgtacgcc attcctgacg gtgatgtgaa aggtcgcgtg gtagcgattt tacttaatga
    23161 tgaagtgaga tcggcagacc ttctggccat tctcaaggcc ctgaaggcca aaggcgttca
    23221 tgccaaactg ctctactccc gaatgggtga agtgactgcg gatgacggta cggtgttgcc
    23281 tatagccgct acctttgccg gtgcaccttc gctgacggtc gatgcggtca ttgtcccttg
    23341 cggcaatatc gcggatatcg ctgacaacgg cgatgccaac tactacctga tggaagccta
    23401 caaacacctt aaaccgattg cgctggcggg agacgcgcgc aagtttaaag caacaatcaa
    23461 ggtcgctgac cagggtgaag aagggattgt ggaagctgac agcgctgacg gtagttttat
    23521 ggatgaactg ctaacgctga tggcagcaca ccgcgtgtgg tcacgcattc ctaagattga
    23581 caaaattcct gcctgatggg aggcggcgca attgcgccgc ctcaaatgat ttacatagtg
    23641 cgctttgttt atgccggatg cggcgtgaac gccttatccg gcctacaaaa ctgtgcaaat
    23701 tcaatatatt gcaggaaaca cgtaggcctg ataagcgaag cgcatcaggc agttttgcgt
    23761 ttgtcagcag tctcaagcgg cgcagttacg ccgcctttgt aggaattaat cgccggatgc
    23821 aaggttcacg ccgcatctgg caaacatcct cacttacaca tcccgataac tccccaaccg
    23881 ataaccacgc tgagcgatag cacctttcaa cgacgctgat gtcaacacat ccagctccgt
    23941 taagcgtggg aaacagtaag cactctgacg gatagtatta tcgataaacg ccggatgaca
    24001 catcacctcc agcgaacgat caccccgatg gcttgcgtca tccagcactt gcaggaacag
    24061 tgcctcgcta atctcttcac cataaaacgc gctactgaat ccctgagaac tgcgcagatt
    24121 agccggtaaa tcaccagagt tagatagcgg ctgacggtcg atgcgcaacg caatcccctc
    24181 ttcagccgca aacctggcaa caatcgggaa aatctgcggg aacatatgca catgatgatg
    24241 gctatcaaga tgcgtaggtt tgcgtccaaa tagctcaatg aaacgcaaat actgactggc
    24301 aagctcctga gtaatttctt ccagcggtaa agcatcttct tctgccaact gccagatcca
    24361 ttttcccagc acaccatcgc gggttaaccc cggcatagct gtcagtggct tgcccatagt
    24421 aaggacaaag tacatcccta tggccagact cggttcatca cgactcaact gcaccgcatg
    24481 gtcaatagcc tgcccattca ccagcgccgt cgtcgatgtg acaatcccat tgcgacaggc
    24541 ctcgataatg ccgtagttct gacctttgct taagccaaaa tcatcggcat taacaatcag
    24601 taagcgttcc ataatcagcc tcggttaatg tgctttttta agctctgcga tgcagtcggc
    24661 aaaatttggc agccatttct cgtgcgccag aatcatctcg cgtgccagca gctcagcatc
    24721 gcgatcggaa tgcaccaacg gactaaggtt tagcgccagt aacacatcgt taaattctcc
    24781 gctaagtgcg gcgttgctgg cagcaatctc gaagccttta atggtgtgaa tcagccccat
    24841 cactttatca tcgaaatgcg taatgcgcgg atgtggcgtc gcgccatcgc gccccagctt
    24901 acaggtcatt tctaccgccc agtctgccgg aatattatca atatgcccat gatgcgggat
    24961 attaacgtaa tgttcagctt gcttgtcgtt gtagatagcg ttgatcactt cgcacgctgc
    25021 atcagagtaa taagccccac cgcgctgttc cagttctttc ggcttaactt tcaactccgg
    25081 gtttttatac agctcaaaaa gttgtttctc gactttctgt actacctgcg ctcgtgcgcc
    25141 gcctttgtag tattcgccca tttcaatagc cagcatctct ttttgcttga agtaataaag
    25201 caaataagaa cacggcaaca gattcagaga acgaattaag ccttcgctaa atggcagatc
    25261 gaaaatattt ttaacgccag atgcttttaa ctgcccggat gccacaccat caagcaattc
    25321 ggcaaagcgc gacttgccat ttaccagcac atccttaatg aacaccatat ggttgaggcc
    25381 gaacaaatcg atagataaat catcgctgtc tttcagcatc agaacatcgt gaataaacat
    25441 cttcatgccg atcggaatat tacacacgcc gataaagcgt ttaaatccgg tatgacgata
    25501 aacggcttca gtaaccattc ccgccgggtt agtgaagtta atcacccatg cattcggaca
    25561 aagttcttcg acatctttta cgatgtcaaa aatcaccgga atggtacgca gacctttaaa
    25621 cagaccgccc gcgccgttgg tttcctggcc aagataacca tgacttaatg gaatacgttc
    25681 atccagttcg cgcgccggta attggcctac gcgcagttgg gtagtaacga aatcagcatc
    25741 tttcaatgct tcgcggcgat ccagcgtttt ataaagcttc atcgggacgc cagcgttatc
    25801 aatcatccgt tggcagagtt caaaaatgat atccagtttc tctttaccat cttcgacatc
    25861 caccagccat aattcgctga ccggcaattc gtgataacgc ttaataaatc cttccagtaa
    25921 ctccggggta tagctgctcc cgccaccaat agtgacgact tttaatttct ggctcataat
    25981 ttctcccttc agtacagaat actgatatct ggcatatctg cccccccgga cataaataat
    26041 ccagcaacag gacagatatg tgaattgtca ggtataacga cttactgatt aaattcagtc
    26101 aatttcttac gatagctctt aggcgtaaag gacgttaatt tcttaaacgt tttaataaac
    26161 aaactcgggc tactataacc ggcctcaaac gcaatatccg tcacggaata gttggtcatt
    26221 tccagttgtt ttttggcaaa attaatacgg atctcattaa taatctgcat tggtgttttg
    26281 ccataatatc gttgagtcgc tcgcgtcaaa tattcctgtg atttggctga caacgtcacc
    26341 atattctcca gcgccgattc actaaactgc tctttatcat gcatcttttc taccgtactt
    26401 ttcagccact gcggtacatc atcaatcacc tgttcttcgc ggtaatgacg taaacggtta
    26461 atgacataaa aagtaaccat ctcaacaaac tcttccagcc ctgtttcgcg gaaattcaat
    26521 gaagaaatca ctgtttccac ataggtgaga aaagcgttat tggtccggta tacctgcgaa
    26581 gcgacaaagc aataaggcaa caacggcagg taatgctgct caaaaaagcg tttactgatc
    26641 ccaacgttca atatacgcgt ggcaccaaac tcataaaaac tttgatggtg cgaacctaac
    26701 ggaataaaaa caaaatcgcc ccgttccagt aacacgcgct taccgttaat ctcctggaaa
    26761 taacgcccgg ttaataccag agtaaattca taatagtcgt gctgatgcag cccgctgata
    26821 ctctcagttt tgttatagat aaacacatgg aagtttttgc cattaaacaa ctgctgttct
    26881 cgggcagtgg caatttccgg cgcgttaatc actggctgca tcatcgactc cttatgcctt
    26941 cagtttttca tgaagctcaa ttaattcagt aatcagttca cgcgcaagca tggacgtcat
    27001 taaatgatcc tgagcgtgga ccagcaccag actcactttc atcttacctt cgcccgcatc
    27061 gccttcaatc agtttcgtct gtaccagatg cgcttcattc aatgccatcc gtgactgatc
    27121 catcatggct tttgctgcag caaaatcgcc ctgcttcgcc tgtttcagtg ccgcatacgc
    27181 caggctgcgc gcttgtccgg agttgatgat aagccccatc accacttctt ccagctcttc
    27241 agcttccgtt tgcgtatcgg gaatgttatc gagatccatc atacatcgtt cctctctatt
    27301 cttaccggca cgattacccg taccggcatc gattaaaatt tcagggcgtt agcgatatct
    27361 tcttcgcgct cttctttatc aatcgcgttt tgtgctttgt tagccaccac aacaaagggc
    27421 agataaatta acgttgcgat accaaggttg aagagtgcaa ccagcaatgc ggcgacgcta
    27481 ccgttggtgt taaagaaggc tcccagaccg gttggcatgg tccacggtgc aatattggtc
    27541 accggaggaa tgatgcccat gtagtacgct gcgagggtga ttgccgccag aatcggttgt
    27601 accagtacaa acgggataaa catcaccggg ttcatgataa ttggcagacc aaacagaatc
    27661 ggttcgttaa tctggaagat gccggacggc agcgccagtt ttgccacctg acgataatca
    27721 gcacggcgag aggcgataaa gatagccagg atcaggccta aagtcgcacc actgccccca
    27781 aggaaaataa aggagtccag catcggcttg gcccagatat ggaaggtctt accggctgcc
    27841 agcgccgctt cgacggaacc atattgctga taggtcgcga tattttccag cgcccacggc
    27901 gtcataatgc cgttgtccag tgcggtcagc gccagcgcgc catgaatacc gaagaaccag
    27961 agcagtggaa caaagatcac ataggcccag cccaccacgc tacccaacga tgccagtggg
    28021 gttgagatgg tatccataat gatctgatgg aagttggtgc cccaggtatt caacgcccag
    28081 gcaataatcc ccatcacgga aagaataata aagccgggaa ttaatgccga gaaggaacgc
    28141 gatactgaag caggtacgct atcgggcaat ttaatgaccc aattgcggcg gacaataaag
    28201 gtaaacattt ctgccaccac caggccaata ataatcccgg agatgatatt cgctccacct
    28261 aaccagtttg cgccaaccac ataggcctca ccgacactat atggggtgac ggtcataaat
    28321 gctgcaacgg ataacaaccc agccgccagc gcatcgactt tacgctcttc tgccagcgcc
    28381 atgccaataa agaacggtgc cattaaagac attatcccta atgttccgtt atatacgttg
    28441 ccgccaatac ctttcaggcc attaagtgtt tcaatggttg aggcgtcgag gcgaatacct
    28501 aaggaataaa aaaacgcccc ctccccaaag cttagaaaaa cgttgttaat taatacaaac
    28561 atggcccccg caagggttaa cggcattaag cgaataaagc catttttgat tgcattaacg
    28621 tgtggctgct ttcctatttt aactgcaaaa gggaggagta ccttttcaag cgatgcaata
    28681 acattactca tagaaaaata cccttaaaaa ccgcaattta aatattgcgg tattgattta
    28741 tgaaataact ctttgacggg aaaatttaaa ataaattaat ttgctgcggc ttttttaatc
    28801 gctgcaaccg cagccttaag cacgcctaaa ccatcgactt tgccataaag cagcgagtca
    28861 attacttcaa ccggtttgtt gggtaacaaa cgctggattt cgggcaacat ataagcaatc
    28921 tgcggcccta ataacacgac atcggcattc tgaccttttt caccagccag tgtttccgga
    28981 aatgcttcaa taatgaccgg aacttcatat ttttctgcct gtgcgcgcat ttttgatacc
    29041 agtaaagagg tagacatgcc cgcagaacaa aacagataaa tgtgtttctt ttccataaaa
    29101 ctgccctcat cgacgattat ctgtcagcca gacactccgc aagccttaac ctgcttccat
    29161 gctctgggta acttgcgaaa ccaacatgac tttttttgtg gctgaaagga gtatacggta
    29221 tagacctgag caatagtatt tctttgacct tataaattgt ttctccttga cctgtggtta
    29281 tgaccctctt cacatttcgg gcaaataatt cgcgaagata attaagaact caggccaaaa
    29341 aaaaccggcg caatggccgg tttccgttgt tactcaagct ttcagacgaa ttgattactt
    29401 cgcagcctgt ggatcagtgt cgtattcagc acaggtctga taaccggagt tgatgacatg
    29461 tccggtatca tctaacgcga caaagtaggt ttctgcttta ccatcacgtt gacccaggat
    29521 gtaggtctgg caagtaccac gagcatggat catgctcact tcagacgaag gtttacccgc
    29581 aatttgtgca acctgcgccc ggctcatgcc ttttttgacg tctttcacca caggctgtac
    29641 aaactggtct ttagtacgat cataagccgt acaacccgcc agcatggtta ataccgccgc
    29701 tgcactcaga attcctgcca tattcttgtt catattccgt cctcttgttt atcagcgtgt
    29761 tagataagcc tggaatacat tgggcgcttt ttcaagcccg cgaacgaaac ggctccgctt
    29821 tcagaggatt cctgtatgac gttttaacca ccattcagcc cgctgtcgct tgtcgtttca
    29881 gtggcaacgg gttagcttta aggaagtttt gtcttttctg tctggagggg ttcaatgaca
    29941 ttgcaacaac aaataataaa ggcgctgggc gcaaaaccgc agattaatgc tgaagaggaa
    30001 attcgtcgta gtatcgattt tctgaaaagc tacctgcaaa cttatccgtt cattaaatca
    30061 ctggtgctcg ggatcagcgg cggtcaggac tccacgcttg ccggaaagct gtgccagatg
    30121 gcgattaatg agctgcgcct ggaaaccggc aacgaatcac tgcaatttat tgccgtacgc
    30181 ctgccctatg gtgttcaggc cgacgaacaa gattgccagg atgccattgc ctttattcaa
    30241 ccggatcgcg tattaaccgt taatatcaag ggcgcggtat tggccagcga acaggcattg
    30301 cgggaagcag acattgaact gagcgatttt gtccgtggca atgaaaaagc gcgtgagcgg
    30361 atgaaagcac aatatagcat tgcgggtatg accagcggtg tcgtggtggg caccgatcat
    30421 gcagcagaag ccattaccgg attcttcact aaatatggtg acggcggtac agacattaat
    30481 ccgctgtatc gtctcaacaa acgtcagggt aaacagttac tggcggcatt aggttgcccg
    30541 gaacaccttt ataagaaagc gccaacggcc gatctggaag atgatcgccc ttctctgcca
    30601 gatgaagtgg cactcggcgt gacctatgac aatatcgacg actatctgga agggaaaaac
    30661 gtacctgaac aggtcgccag aacaatagag aactggtatc tgaaaaccga acataaacgc
    30721 cgtccgccaa ttaccgtttt cgatgatttc tggaaaaagt aataatttgc acataatggt
    30781 ttgtgcgaaa aagagtaatt tgttcacgcc ggatgcggcg tgaacgcctt attcgaccta
    30841 caaaactatg caaattcaat atattgcagg agtcgaatag gcctgacagg cgtagcacgt
    30901 cagacggtgt aacctttgtc atcgacccgc ctctttttta atcgctcccc gcctgttaca
    30961 ctggatagat aaccagcatt cggagtcaac agtggtacgg cgtttaactt ctccgcggct
    31021 cgaatttgaa gctgcggcaa tttatgaata tcccgaacat ttacgttcat tccttaatga
    31081 cttacccacc cgaccagggg tgtatctgtt tcatggcgaa agtgacacca tgccgctcta
    31141 tatcggcaaa agcgttaata tccgcagtcg ggtgctttct catttacgca ccccggatga
    31201 agccgccatg ctacggcaat cccggcgaat tagctggatc tgtaccgcag gcgaaatcgg
    31261 cgctctgctc cttgaagcgc gattaatcaa agaacaacag ccgctgttta ataaacggtt
    31321 gcgccgcaat cgccagctct gtgccctgca attaaatgaa aagcgcgtcg atgtggtgta
    31381 tgccaaagag gtggattttt cacgagcccc caacctgttt ggcctgtttg ccaataggcg
    31441 cgcagctttg caagcattgc agaccatcgc tgatgaacaa aaactttgtt atggcctgct
    31501 gggactggaa ccgttaagtc gcggtcgtgc atgttttcgt tcagcgctaa aacgttgcgc
    31561 cggagcatgc tgcggtaaag agagccatga ggaacatgcg ctacgcttgc gccagtctct
    31621 ggagcgtttg cgggtggtgt gttggccttg gcaaggtgcg gtggcactga aagaacagca
    31681 cccggaaatg actcaatatc atattattca aaactggctg tggctggggg cggttaattc
    31741 gctggaagaa gcgacaacgt taattcggac acccgccggg tttgatcacg acggttataa
    31801 aattctttgt aagccgctgc tttccggtaa ctatgaaatt actgaacttg atccggcgaa
    31861 tgaccagcga gccagttgat ttcactgaac aacagtttac cttgcggttg taaaagccgc
    31921 agggtgtgcc tgccatcgaa ccagcacgcg ccctggtcgg tagtgagcaa tttatcgccc
    31981 agttgccacg ccccgttaat cacaaacacc accccgccgc gtgagccaaa ggtagtaaac
    32041 gtacgttcgg caattctgac tttcgcctta cagacatcca ggcgtgtcat gatgttgaaa
    32101 tccatcgaca tctgcccttc cgtcagtttc gcttttacca cctggtccgc tgcaaaggca
    32161 aaaggctgta acggttttaa ggtatggtta aagcggtctg cgctttcaag gagcatctcg
    32221 ccgccttcca gcaacgtcac tatcctttcc atgccgggaa acaaagaaaa ctccccattc
    32281 gccgcgatgg atgcaatgct ggcacgccag taaaaatcac gttttgccgg tgggaaagtg
    32341 caaatttcgc gcgtttcacc ggcagcattt cgccacagat tcaccgacat tttacgcata
    32401 tcaaagtatt ccatactcgc tcccggcccg ttcttttcat tgttatcgtt caggtaaccg
    32461 tggatggtta tcggcaactc gggtgattta cgttagtggt gatcaggctt tttttacaac
    32521 tgtttgagaa gagaaaagaa aaccgccgat cctgtccacc gcattactgc aaggtagtgg
    32581 acaagaccgg cggtcttaag ttttttggct gaaagattat tcagcagttg caggcatttt
    32641 accttttg
//
LOCUS       DAESHI010000040        32647 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.040, whole genome shotgun sequence.
ACCESSION   DAESHI010000040 DAESHI010000000
VERSION     DAESHI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32647
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.040"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..304)
                     /locus_tag="KW011_001915"
     CDS             complement(<1..304)
                     /locus_tag="KW011_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBH7660255.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
     gene            complement(514..1032)
                     /locus_tag="KW011_001916"
     CDS             complement(514..1032)
                     /locus_tag="KW011_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HBH7660256.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            1729..2229
                     /gene="tssB"
                     /locus_tag="KW011_001917"
     CDS             1729..2229
                     /gene="tssB"
                     /locus_tag="KW011_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HBH7660257.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            2264..2488
                     /locus_tag="KW011_001918"
     CDS             2264..2488
                     /locus_tag="KW011_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660258.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            2539..4014
                     /gene="tssC"
                     /locus_tag="KW011_001919"
                     /pseudo
     CDS             2539..4014
                     /gene="tssC"
                     /locus_tag="KW011_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            4021..4434
                     /gene="tssE"
                     /locus_tag="KW011_001920"
     CDS             4021..4434
                     /gene="tssE"
                     /locus_tag="KW011_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HBH7660259.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            4438..6288
                     /gene="tssF"
                     /locus_tag="KW011_001921"
     CDS             4438..6288
                     /gene="tssF"
                     /locus_tag="KW011_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HBH7660260.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            6252..7334
                     /gene="tssG"
                     /locus_tag="KW011_001922"
     CDS             6252..7334
                     /gene="tssG"
                     /locus_tag="KW011_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HBH7660261.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            7359..8639
                     /gene="tagH"
                     /locus_tag="KW011_001923"
     CDS             7359..8639
                     /gene="tagH"
                     /locus_tag="KW011_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HBH7660262.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            8636..9160
                     /gene="tssJ"
                     /locus_tag="KW011_001924"
     CDS             8636..9160
                     /gene="tssJ"
                     /locus_tag="KW011_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HBH7660263.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            9163..10494
                     /gene="tssK"
                     /locus_tag="KW011_001925"
     CDS             9163..10494
                     /gene="tssK"
                     /locus_tag="KW011_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HBH7660264.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHHARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKIFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPCIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            10499..11260
                     /gene="icmH"
                     /locus_tag="KW011_001926"
     CDS             10499..11260
                     /gene="icmH"
                     /locus_tag="KW011_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HBH7660265.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            11269..14094
                     /gene="tssH"
                     /locus_tag="KW011_001927"
     CDS             11269..14094
                     /gene="tssH"
                     /locus_tag="KW011_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HBH7660266.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTMTDASDTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESESESESESESESESESEQDNTGAEPADEADREQPE
                     ETAETVSPVQRLAQLTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEM
                     SVITDLTKWLGDTIKGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTET
                     VLQLAELLYGGRQYLTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYS
                     VVLLDEVEKAHPDVLNLFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHAD
                     DPETMQEVLYPVLADFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFG
                     VEVVIEPEVTDEIMSRVTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRL
                     SAVDGAFTADVEDAQNDESVTKDETVL"
     gene            14091..14834
                     /gene="tagO"
                     /locus_tag="KW011_001928"
     CDS             14091..14834
                     /gene="tagO"
                     /locus_tag="KW011_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HBH7660267.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            14839..16251
                     /gene="tssA"
                     /locus_tag="KW011_001929"
     CDS             14839..16251
                     /gene="tssA"
                     /locus_tag="KW011_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HBH7660268.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGQLAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            16270..16530
                     /locus_tag="KW011_001930"
     CDS             16270..16530
                     /locus_tag="KW011_001930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660269.1"
                     /translation="MFRLPTPRLLSGLKSALRPAMPRFKVSAFWLLILAWIFLLVWIW
                     WKGPMWTLYEEQWLKPLAGNGGVGDYCPGVAYRPGDEAPATA"
     gene            16676..19783
                     /gene="tssM"
                     /locus_tag="KW011_001931"
                     /pseudo
     CDS             16676..19783
                     /gene="tssM"
                     /locus_tag="KW011_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010917757.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            19794..21146
                     /locus_tag="KW011_001932"
     CDS             19794..21146
                     /locus_tag="KW011_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HBH7660270.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQHTRKRLEPQPKSNAENAAQTTVRTLIYAPETLASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYHDARAQKQNTDALEKQINERLEGVLSRWLLLKNNVMPEAATGTTAEK
                     "
     gene            21170..21652
                     /locus_tag="KW011_001933"
     CDS             21170..21652
                     /locus_tag="KW011_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HBH7660271.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            21696..22643
                     /locus_tag="KW011_001934"
     CDS             21696..22643
                     /locus_tag="KW011_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660272.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKKKKKKKKKKKKILSPITSFDYIHFITTHPSGIKDTL
                     AWLVNASKLMTEFDDNGKIIFNINALKYTKASYFEILGEKYIKITTSSPWLLEKLGKY
                     IFSSRAPQVLELAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFS
                     MDVAKAVIAGGVATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHL
                     SEKLKYAIRDGLKRQQELDKWKRENMTPFMYVLNTPPVI"
     gene            22653..23132
                     /locus_tag="KW011_001935"
     CDS             22653..23132
                     /locus_tag="KW011_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660273.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFMSFSFLNIHVILLTIIKLFLIKKTKVVNFATDITIQLTLIFLL
                     IAIVIAPLIAPFVTGYVNANYHPCGNNTGIFPGAIYIKNGMKCNNEYISRKEDSAVK"
     gene            complement(23269..24054)
                     /locus_tag="KW011_001936"
     CDS             complement(23269..24054)
                     /locus_tag="KW011_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660274.1"
                     /translation="MNLRRVCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(24384..24460)
                     /locus_tag="KW011_001937"
     tRNA            complement(24384..24460)
                     /locus_tag="KW011_001937"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24424..24426),aa:Asp,seq:gtc)
     gene            complement(24593..25324)
                     /gene="dnaQ"
                     /locus_tag="KW011_001938"
     CDS             complement(24593..25324)
                     /gene="dnaQ"
                     /locus_tag="KW011_001938"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HBH7660275.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            25389..25856
                     /gene="rnhA"
                     /locus_tag="KW011_001939"
     CDS             25389..25856
                     /gene="rnhA"
                     /locus_tag="KW011_001939"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HBH7660276.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(25853..26575)
                     /locus_tag="KW011_001940"
     CDS             complement(25853..26575)
                     /locus_tag="KW011_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH7660277.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            26609..27364
                     /gene="gloB"
                     /locus_tag="KW011_001941"
     CDS             26609..27364
                     /gene="gloB"
                     /locus_tag="KW011_001941"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HBH7660278.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            27436..28794
                     /gene="mltD"
                     /locus_tag="KW011_001942"
     CDS             27436..28794
                     /gene="mltD"
                     /locus_tag="KW011_001942"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HBH7660279.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(28843..29439)
                     /locus_tag="KW011_001943"
                     /pseudo
     CDS             complement(28843..29439)
                     /locus_tag="KW011_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(29470..30270)
                     /locus_tag="KW011_001944"
     CDS             complement(29470..30270)
                     /locus_tag="KW011_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBH7660280.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            30511..31425
                     /locus_tag="KW011_001945"
     CDS             30511..31425
                     /locus_tag="KW011_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH7660281.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(31422..32225)
                     /gene="dkgB"
                     /locus_tag="KW011_001946"
     CDS             complement(31422..32225)
                     /gene="dkgB"
                     /locus_tag="KW011_001946"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HBH7660282.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(32388..32464)
                     /locus_tag="KW011_001947"
     tRNA            complement(32388..32464)
                     /locus_tag="KW011_001947"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32428..32430),aa:Asp,seq:gtc)
BASE COUNT         8229 a         8387 c         8390 g         7641 t
ORIGIN      
        1 tgcgccacag cggtgggcat accttcatgc tgtacagcat ctggtttttg tcgttctccc
       61 ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacgtcatcg ccctgccata
      121 tcgtcaggta ggccattttg tccagtacct gcggaaattc aagggagaga aactgctggc
      181 tgaccagaga gagctcaagg gaaaagagcg aagagagtga ctggttcaga tgaaaggaga
      241 ccaccgcaaa agcatccggt ggcaggccgt ccacttccag cgtgaaacgt aatccggttg
      301 acatagcaac ctccggggtt aaggaaaaac agacagttcg ctgttaaagc ggctgtgaca
      361 gtacagtgtt acgcttgcgt tcaggcacgc taccgccgaa gtttccggca tccgccatct
      421 cccgtgaacg taacccctta ctgacaacct ttctttctac ggcgtaatac aaaaacagca
      481 tccggcctga tagccggatg ctgttggctt aacttatgct tccagtggtg cgcgccagtc
      541 gtcggcaccg gaggtgccag atttgacgtg ttcccattca attttgcggt aggccagtga
      601 aacctcgagc aactgggtga actcgcgttt cgccgggtcc tggcaatgcg gcatatgcag
      661 tttcatatcg acaatcgtcg aatcagtcag gcgggtagtg aaataatgct cctgtttgcc
      721 ctccacagag gtacgccacc agtgaagttc agtggttggc agcatttcgc cggaggccag
      781 tgcgttgtac atcagcggaa ccgctttgtt cagcgccacg gtaaaaatga aaggcttgtg
      841 agcacgctga cccgcaggct gaccggactg aggagtggtc gggacagtaa cgttatgcag
      901 gaattcctgc accagcatct cgtcttcgtg cccctgcaca tagatgttgc cgacagaatc
      961 agcggtaaat gcacctgcag taatatttcc ctgggtctga ccagtgatgg agatataaca
     1021 cggtgttggc atttgtaaac tccttgttaa cgtggttaat tactacttac aatgacctgc
     1081 cgttaatccc agacaggcag atgcagctca cttcacggaa ccgcatcatt tttcagtatt
     1141 ttttacatac aaaccgagct agaatttacg gttgtcgtaa aaactatttt taaaatcatt
     1201 aaatctctta ttcagcaata acttaataat atatatctgg ataaccatac ccaactgttc
     1261 aaagtccgta ttgcctccat aaaatggcca aagctgaatc atgaataaat attgtattta
     1321 tcctttctga attatcattt tgataatttt tgagatattc cttaaaaata cccacttaat
     1381 caatcacaaa acaaatatat aaacaagcaa tcattagatt agcttatttt taatgaaatt
     1441 attatatact tcaataagtt acacaaaaac tctatattta aattattgcc ataatacacc
     1501 tgacgcgaat tgcacaggtt cacacaatct atttaacgct tgcagaatac ccctctttac
     1561 gatcgataaa aagtcctttt cttatttatt ttgaagcgta aaatgcgggg tgatgaagga
     1621 gtggtgattt tattttcacc gccattaggg cggcattgcc gatatttttt tgcggtacaa
     1681 atatgccgtg ttcatggcct gtacgagata acatgacgga tcacttttat gagcaaaaaa
     1741 tttgaaggca gcgtcgcgcc ccgcgagcgt attaatatat cgtatgtccc aaaaacagat
     1801 ggccagacgg ctgaagtgga acttccgctt aacatgctcg ttgtcggcga taccggaaat
     1861 acgcaggaaa catccccact ggatgaacgt caggcagttt ccgttaacaa gcacaatttc
     1921 ggtgcagtga tggctgaagc agccattggc ctcaatttta ctgtccccgc cacgctgaag
     1981 ggaagcacaa cagacgatga actgaatgtg gcgctgaata tcaaatcact ggatgatttt
     2041 tcaccagata gcgtggcccg gcaggttccg gaagtgaata aattgctgga actgcgtgaa
     2101 gcactgactg cactgaaggg gccgatgggt aaccttccgg cattccgtac ccagctccag
     2161 gcattgctgg aaaatgaaga gtcccgcgag cagttgctca aagaaatcgg tctggtgagc
     2221 aataaataac caccactatt tcattaaaca gggttatcca ggcatgacgg cacacaaaaa
     2281 tatctccggg acataccacc tctctgttgc agacattctg caggtggttt accaggtatg
     2341 tttcagcccg tccgttgaaa tcaaccagga tggtgtggct gcgctgatta caacactgga
     2401 tcgccgtatc agtgatctat tggatgagat tatccatttt tgtgagtttc agcagagcgc
     2461 gtcgcactgg cagcgcgtac ttcactgatg gcgaaaagcc ggtttcagaa acacagctaa
     2521 aacagaagga ttttcagtat gtctttacag gaagaggaac tcgtttccag tcatgccggg
     2581 cagcctgagc aggcatcgtc gctgcttgat caaatcatgg cacagacccg tattcagccc
     2641 ggttcggaag gttatgacgt tgccctccag ggtgtgacag cctttattgc cagcatcctg
     2701 caatccactg catctgccga accggtcaat aaactggccg tggacagcat gattgcagat
     2761 attgatgaac gcatcagccg ccagatggat gtcatcatcc atgcgcctgc ctttcagcag
     2821 gtcgagtcgt tctggcgctc actgaaaacc atggtggatc gcgttgattt ccgtgaaaac
     2881 atcaaggtca acgtgcttca tgtcaccaag caggaacttc tggaagactt tgagtttgcg
     2941 ccagaaatta ttcagtccgg attttataag cacgtttatt cgtccggttt cggacagttt
     3001 ggcggtaaac ccatcgctgc cgtgctgggc gcttatgagt tcaaaaatac cgcgcctgac
     3061 atgaaactgc tgcaatacgt cagtgccgtg ggcgcaatgg cgcacgcacc gttcctgtcc
     3121 tccgtttccc cggagtttat ggggctgaac tcgtggaccg aactgcctaa tatcaaagat
     3181 ctttatgcca tctttgaagg tccggcctac accaaatggc gcgccctgcg tgactcggaa
     3241 gattcgcgct atttagggct tacggcaccc cgcttcctgc ttcgccagcc ctattcacca
     3301 acagataacc ctgttaagaa ctttaactat tacgaagatg tcagccagaa ccacgaagat
     3361 tatctgtggg gtaatacggc ctggatgctg gcatgcaata tcgcagacag ttttgccaaa
     3421 taccgctgat gtccaaacat tatcggtccg caaagcggcg gcgcagtgaa agatctgccg
     3481 atgcatctgt tcgaaacgat ggggcagatt caggccaaaa tcccaaccga ggtgctggtc
     3541 accgatcgcc gcgaatttga actggctgaa gagggtttta tcacccttac catgcgtaaa
     3601 gactctgata atgcagcctt tttctctgca aactcggtac aaaaaccgaa gcactttccg
     3661 ggaaaagatg cagaaaccaa ctataagctg ggtacgcagc ttccttatct cttcatcatc
     3721 aatcggttag cgcactacat caaggtgttg cagcgtgaac aactggggtc atggaaagaa
     3781 aggagcgact tagaaagaga gcttaatacc tggatccgtc agtatgttgc cgaccaggaa
     3841 aacccgcctg cagacgtgcg cagccgcaaa cctctgcgcg ctgccaaagt tgaggttatg
     3901 gatgtagaag gcgaacccgg ctggtaccag gttgcgctaa gcgtgagacc tcattttaaa
     3961 tttatggggg caaattttga gctttccctg gttggccggt tagacaggga gtaacacacc
     4021 atgataccgg atcgggaaag aaccacaggt tctctttttg agcgtatgga agcctcttcg
     4081 gccagaaacc ggcagggagg cagtattcat tcgctcaggc aatccattcg ccagaaccta
     4141 cgaaacattc ttaatacccg gtccggtagt tgccggggtg cgcctgagct gggcattgat
     4201 gaaccggaag gggctgaaaa cttccgcgaa tccatgagtc gggccatcga gcaatgtata
     4261 gaacgctatg aaccccggat ctcacacgct gaagtgcagg cagtggtttc atccgcctcc
     4321 agcccgctgg atatgacgtt tcatattaca gcctgggtga catttaacga aacgcatgag
     4381 gtgcttgaat ttgatatggc accgaatggc agccagcatt accgggtgga ctgacaaatg
     4441 gaatttgaag aacgctattt ccgggaagaa ctcgattacc tgcgccagct tagcaagctg
     4501 ctggcaacgg aaaaacccca tctggcccgc ttcctggccg aaaaagatgc agatccggat
     4561 attgaacgcc tgctggaagg ggtggctttt cttaccggca atctccgcca gaaaattgag
     4621 gatgaattcc cagaactgac gcacgggctt attaagatgc tatggcctaa ttacctgcgt
     4681 ccggttccgg caatgaccct tattgaatat acgccggata tggataagtc ttctgtaccg
     4741 gtgttaatcc cccgtaatga gcagtttaca accaacgccg gggaaatcag agttgatgaa
     4801 gtgctgccct ctgatgctaa aaaggaggag ccgcctccct gtaccttcac cctctgccgg
     4861 gatatctggc tgctgcccgt tcgcctggag cagattgaaa accgcagtac gacccgtaat
     4921 ggtgttatca acatcacctt ttcggtcgca ccgggaacgg acttccgcac gctggatctg
     4981 aacaaacttc gcttctggct cggcaatgac gacaactata cccgtgacca gctttattta
     5041 tggttctgcg aatacttgca gggtgccgac ctgactgtgg gtgaacagca tattcgcctg
     5101 cctgagttta tgctaaaagc tgtcggtttt gagccgcagg atgccatgct gccctggccg
     5161 aaaaacgtcc acagcggcta ccggatcctt caggagtatt tctgttaccc cgatgcgttt
     5221 ctcttttttg atctttgtgg ttgtccggct ttgcctgacg gattgcaggc ggaattcttt
     5281 accctgcaac tgcgtttttc gcgccctttg cccgtggaca tccggctgcg ccgcgattcc
     5341 ctgcgcctgt attgcgcacc tgccattaat ttatttatcc accatgcaga agccatcacg
     5401 ctggacaacc ggcgggcaga ctatccgctg gttcccagcc gccattaccc acaacattac
     5461 gatgtatttt ccgttaacag tgtggtgagc caggtccagg atatgttcag gaaaaaagat
     5521 ctggggcgtc ctgtttcgac gcaggccgcg cgccagtggc cagcctttga aagtttcagc
     5581 catcagatag aatacagccg gaagcgggaa gtggtgtact ggcatcaccg gaccaaaaca
     5641 tccctgttcc atcgcggctt tgatcatacc cttgccttta tacatgctga tggcagttat
     5701 ccgtcagacg aatctctgct cagtaatgaa gtggtttcgg tatcgctgac ctgtaccaac
     5761 cgtgagcttc cgtcacaaat tcgttccggc gatatcaccg gcacaaccgg aaaaaatgcg
     5821 gctgttgctt catttcgcaa cattacccgc ccgacgcaac cactctggcc ggtcattgat
     5881 ggcagcctgc actggcccct actctccgcc atgaacctga attatctgtc attactggat
     5941 acggacgcgc tgaagcaggt catcgccaac tttgatcgcc acgcaatcca tcatccgcag
     6001 acggcacggc tgtcacaaca aaagctggat gccattgagc gtctggagac ccgccccgtt
     6061 gatcgcctgt ttacgggtat tcccgtccgg gggctggctt ccacgctgta tctgcacccg
     6121 gagccgtttg tctgtgaagg ggaaatgtat ctgctcggta cggtgctttc gcattttctg
     6181 tcgctgtacg ccagcgttaa ctcattccac atgctgaccg ttgtgaacac agaaagccag
     6241 gagacatgga aatggacgga aagaatcggg cagcatcctc ttatctgagt ccgggaaatc
     6301 ccccggcaga taaagagcag aatgatccgc tggcacaggt tttccataat gcctgctctt
     6361 acaatttttt tgcgatggcg gagctgttgc accgcctggc aaagggtgaa aaggggacgc
     6421 cagaattatc cctgcgcgac gatccggcac aggaaaccct gcgtttcagc gccgatgcca
     6481 gccttgcctt cccgtgcagt gatatcagtg cgctgaaacg ggatacgtca ggggcattca
     6541 ggatgaccac cacctttatg gggttacagg ggagccagtc gcctctcccc ggttattacc
     6601 tcgctcacct ggcctggaaa gcggtacatg aacaaagtcc ggtaggtgat tttctggata
     6661 tgttcagcca ccgcctgacc cagtttgtct ggcatatctg gcgtaagtac cgctatcaca
     6721 tcagttttcg taacggcggt gtggatgcgt tctcgcagcg catgtactcc cttgtcgggc
     6781 tgggccaccg ccagcttcgc gataagctgg cgattaatca cagcaaaatg ctggcgtatt
     6841 ccgggatcct ggcaaatccg ggacgatccc cggaaattat ctgtggcctg gtgtcccact
     6901 gctttgattt gtcagaagtg acactgcaaa actggcaacg tcgcaaggtg gatattgaac
     6961 cggatcagca aaatagtctg gggagctatt ctcttaaaaa tggcgaaaaa ctggcgggaa
     7021 gatcggtact gggcaacttt gttctgggta cacgagtgcc cgatctcagc ggaaaattcc
     7081 agctcagtat taccagcctg acccgaaaac agttcctctc ttttctgccg tccggcgaaa
     7141 acttcctgcc actgacgatg ttcgtgtcct tcatcctgcg cgatcagctt gcctgggact
     7201 tacatctggg gctggcgccg gagcaggtgg gcgcaatgcg cctgggcgat aacaaaagtg
     7261 cgctgctggg ctggaccagc ttcctcggca caccggaaga acgaccctca gtcaccatca
     7321 gggtacggtc gtaaattatg ataatcacgg agcagaatat gccggaagag aaactacaga
     7381 cgctttcatt gcaggtcatt aacggcagtg agctggaaag cgggcgggcg gcgcgctgtc
     7441 tgttcacaca gcagggaaat gtcggccatg gccccgaatg ccactggtcg gtacaggatc
     7501 gtcagcagag cattccggcc caggctttta ccgttatcct gcacgatggc acattttgtc
     7561 tgcgcccgca gacggcacaa ctgtggctga atcaggcaaa agtcacagca acatcagacc
     7621 taatacagtt gcgccagggc gatgagatcc agatcggacg gctgatggtg agggttcatc
     7681 tgaaccgggg agatattccc cattacgatg aggaaatggc cactcccgaa accatcgtta
     7741 ccaatcgcga tatgctcacg gataccctgc tatcaacgga gggtgcgcca cactatccgg
     7801 gaatgactca ccggcaccag cttgcagaca ccgtggtaaa cggtttttct gccgatccac
     7861 tccaggctct tcagtccgaa agcctgatta ccacgggcga tccgctttca ggcattgcgg
     7921 ctgtccggcc atcggcaccg ctgtccgatc cggcaagtaa tggggggatc aatactccgt
     7981 ttatggatct gccgcccatt tatgccagcc ccggcgatcg taatgacgac gtctctgcgg
     8041 cagaaatggc gcaacgccac gttgcggtca cccccttact gcgcggtctt ggcggctcgc
     8101 ttaccatgag caattccgac gatgcggatg attttctgga ggaggccgga cgaacgttac
     8161 aggccgcaat aaaaggtctg ctcgatttgc agcagcagcg taacagcctc tcagacaaac
     8221 atttgcgccc gctggaagat aacccgctgc gcctgaacat ggattacgcc accgcgctcg
     8281 acgtaatgtt tgccgaaggt aaaagcccgg tacatctggc ggctcccgcc gccgtcagtg
     8341 aaagcctgcg caatgtccgc caccacgaag aagctaaccg ggcagcgatt gtggagtcgc
     8401 ttcgtgtcct gctggatgct ttctcaccac aaaatctgct gcgccgcttt gtgcagtacc
     8461 gccgcagcca tgaactgcgc cagccgctgg atgatgccgg agcatggcaa atgtacagcc
     8521 attattacga agaactggcc tccgatcgcc agcaggggtt tgagatgctg tttaacgagg
     8581 tctacgccca ggtctatgac cgggtgcttc gtgaaaaaca gcgggagccg gaagcatgaa
     8641 caggaaggcg tttctggcct gcgtgctgat gtgcgtcata ttaaccggct gcgaaacggc
     8701 gaaaaaaatc agtgaggtga tcaaaaaccc ggatatccag gtcgggagcc tgaaggagca
     8761 accttcggaa ataaccgtca ccctgctgac cgaaccggac accaatacca atgcggaagg
     8821 tgaatcagcc gccgtggatg tgcaactggt gtacctgact gacgactcaa aattgctggc
     8881 agccgactat gaccagatag ccagcacgcc gctaccggat gtgctgggta aaaactatat
     8941 cgaccaccag gatttcaacc tgttgccgga caccatcaaa accctgccgc cggtaaagct
     9001 ggatgagaaa acgcagttta ttggtgtcgt ggcctatttc tccgatgacc aggccactga
     9061 atggaaacaa atcgagacgg tggaaggaat cggccaccat taccgcctgc tggtgcatgt
     9121 acgtcaaagc agcatcgaaa tgaaaaaaga ggatgaataa ttatggcaac cacccgcaac
     9181 aaggtgatgt ggcaggaagg gatgctgatg cgtccacacc atttccagca gcagcagcgt
     9241 tacaacgact acctggataa ccagcgtttc cgggccatga atgatttatc ctggggattt
     9301 accgaactca ccctcaacaa tgaactgctg gcgcagggta agatcatgat tgacagcgcg
     9361 tcaggcacac tgcccgacgg caccgtcttt tctatccccg accaggacgc actgcccgat
     9421 ccgctgcacc cgcaaaattt tccggacgaa agaagccgca atatctacct cgctctgccg
     9481 gtcgccagtg atgtgagaaa tgaaatcagc gacggacggc gaatcgggcg ttaccggctg
     9541 aattatgccg atgtccggga tttgcattca gaagaaggtg acgcgcgaac attgacgctg
     9601 ggtcaactga cgccgcgcat tatgagcggt gcagaagata tgagcgccta tattacgctc
     9661 ccgctttgcc gtatcagcga tcgccatgcc gatggctccc tgacgttgga tgacgatttt
     9721 atcccctcct gccagaatat tcaggtcagt aagaaactgc gtgtttatct caaagaggta
     9781 cagggggcca ttggcggacg ggcaagcgat ctggcaaacc gtattggctc tccggcgcag
     9841 agcggcatcg cggatgtggc ggaatttatg atgttgcagt tacttaaccg taaccagacc
     9901 cggtttaccc atcacgcccg tcgatcccag ctccacccgg aagatttcta ccttgatctt
     9961 gccgggttgc tgggtgaact gatgaccttt accgagccgt cgcgcctgcc ctgcccgctt
    10021 gatgtgtatg atcatcatga cctgaccaaa atatttaaaa cgctgttacc ggaagtcaaa
    10081 cgggcgctgc ataccgtact gtcgccaaga gccgtcaatc tgccgctgca tctgcgtgac
    10141 ggtatctggc aggccgatat ccatgacaca gaactgctgc aatctgccac ctttgtgctg
    10201 gccgtggcgg caaatatgcc ggtcgatcag atccagcgcc agtttatcca gcagtcgaaa
    10261 atttcctcgc cggaaaaaat ccgcaatatg gtcagtgtgc agatcccctg tattccattg
    10321 cgtgccctta tggtggctcc ccgccagctt ccttaccatt ccgggttcag ctatttcgaa
    10381 ctcgacaaga gtggccaggc ctggacagaa atggctgccg ccggggccgt tgcactgcat
    10441 gtttccggca gtttcccgga tctgaacatg caactgtggg cgataagagg gtaagacgat
    10501 ggatgagggg tcactgtctc tgccaccatt taccggacac gacgaaaaaa gccagcgcaa
    10561 ctaccatctc gcgcttcgtg gcaacagcct gaatccgatg atcgacgcgg ccacgccgct
    10621 actcggtatg gtaatgcgcc tttcaaccat gaacagccag acgatgccgg agcatctttt
    10681 tgctcaggtg gtgacagatg ttcaggccgt ggagcaactg cttcaggagc agggctacga
    10741 accgggcgtg attatctcgt ttcgctacat cctctgcacg tttattgatg aagcggcgct
    10801 cggtaacggc tggtcaaaca agaacgagtg gatcaagcag tcattgctgg tccatttcca
    10861 caacgaagcc tggggtggcg agaaagtttt catcctgctg gagcgcctga tccgtgaacc
    10921 caaacgttac caggatctgc tggaatttct ctggatctgt ttttctctgg gatttcgcgg
    10981 gcgctacaaa gtggcagcac aggatcaggg ggaatttgag cagatctacc gccgtctgta
    11041 ccacgttctg cataaactgc gcggcgatgc ccctttcccg ctgttacacc aggacaaaaa
    11101 aacccagggc gggcgttatc agctcatcag tcgcctgact gttaaacata ttttttgtgg
    11161 tggcgttgtc gtgctggcgt tgttttacct gttctatctg ttgcggctgg acagccagac
    11221 gcaggacatc ctgcaccagc taaacaaatt acttcgataa ggacgtttat gatccagatt
    11281 gatcttccca cgctggtaaa acggctgaac ctgttctccc gccaggcgct ggagatggcc
    11341 gcctctgaat gtatgagtca gcaggcagcg gaaattaccg tcagccatgt gctcattcag
    11401 atgctcgcca tgccacgcag tgacctgcgg gttattaccc gccagggcga tattggcatg
    11461 gaagagttgc gccaggcgct gacggtagag aactacacaa ccgcccgttc tgcggacagc
    11521 tacccggcgt tttccccgat gctggttgag tggcttaaag agggctggct gctggcgtcg
    11581 gctgagatgc agcacagcga actgcgcggc ggcgtgttgc tgctggccct gctgcattcg
    11641 ccgctgcgtt atataccgcc tgctgccgcc cggctgttga ccggcattaa ccgtgaccgt
    11701 ctgcaacagg actttgtgca gtggacacag gagtcggcgg aatcagtcgt gccggatgca
    11761 gacggtaaag gcgcaggcac aatgacggac gcctctgaca ccctgcttgc ccgctatgcc
    11821 aaaaacatga ccgcagacgc ccgtaacggc aggcttgacc cggtactgtg ccgcgaccac
    11881 gaaatcgacc tgatgatcga cattctctgc cgccgccgta aaaacaaccc ggtggtggtg
    11941 ggcgaagcgg gcgtgggcaa aagcgcactg attgaagggc tggcgctgcg catcgtggca
    12001 ggccaggtgc cggacaagct gaaaaacacc gatatcatga cccttgactt gggcgcattg
    12061 caggccgggg cgtcggtgaa gggtgaattc gaaaaacgtt tcaaagggct gatggcggag
    12121 gtcatttcct ccccggtgcc ggtcattctg tttatcgacg aagcacatac cctgattggc
    12181 gcgggcaacc agcagggcgg gctggatatc tccaacctgc tcaaaccggc gctggcgcgc
    12241 ggcgagctga aaaccatcgc cgccaccacc tggagcgagt acaaaaaata cttcgaaaaa
    12301 gatgccgccc tgtcgcgccg cttccagttg gtgaaggtca gcgaacccaa cgctgccgaa
    12361 gccaccatta ttctgcgcgg tctgtcggcg gtctatgaac agtctcacgg cgtgctgatt
    12421 gatgatgacg ccttgcaggc cgctgcgaca ttaagcgagc gttatctctc cgggcgtcag
    12481 ttaccggaca aagcgattga tgtgctggat accgcctgcg cccgtgtggc catcaacctg
    12541 tcgtcgccgc cgaagcaaat ctcggcgctg accactctga gccaccagca ggaggcggaa
    12601 attcgccagc ttgagcgcga gcttcgcatc ggactgcgta ccgacacatc acggatgacc
    12661 gaggtgctgg tgcagtatga tgaaacgctg acggcgctgg atgaactgga agcggcctgg
    12721 caccagcagc agacgctggt ccgggagatt atcgcgctgc gccagcagtt actgggcgtg
    12781 gcagaggacg atgcggcgcc gttgccggac gcagataccg tggaggatac gcagccagag
    12841 tcagagtcag agtcagagtc agagtcagag tcagagtcag agtcagagtc agagtcagag
    12901 tcagagtcag agcaggataa taccggtgcc gaaccggctg atgaggccga cagagaacaa
    12961 ccggaagaga ccgctgaaac agtttccccg gtacagcggc tggcccagct cactgccgaa
    13021 ctggacgccc tgcataacga ccggttgctg gtctccccgc acgtcgataa aaaacagatt
    13081 gcggcggtga ttgccgaatg gaccggcgtg ccgctcaacc gcctgtcgca gaatgaaatg
    13141 tcggtcatca ccgacctgac gaaatggctg ggtgacacca tcaaaggcca ggacctggcg
    13201 attgccagcc tgcataagca tctactgacc gcacgcgccg acctgcgtcg tccgggacgc
    13261 ccgctcggtg cgttcctgct ggctggcccc agcggtgtgg gtaaaaccga aaccgtcctg
    13321 caactggcag agctgctcta cggcggtcgc cagtacctga ccaccatcaa tatgtccgaa
    13381 ttccaggaga aacacaccgt ctcgcggctg attggttcgc ctccgggcta cgttggctac
    13441 ggtgaaggcg gcgtactgac cgaagcgatt cgccagaaac cctactcggt agtactgctc
    13501 gatgaagtgg aaaaagcgca cccggatgtg ctcaacctgt tctaccaggc gttcgataag
    13561 ggcgaaatgg cagacggtga aggccgcctg attgactgca aaaatatcgt cttcttcctg
    13621 acgtccaacc tcggctacca ggtaatagtc gagcatgccg atgacccgga aaccatgcag
    13681 gaagtactgt atccggtgct ggccgacttc ttcaaacctg ccctgctggc gcgtatggaa
    13741 gtggtgccgt atctgccgct gtcgaaagag acgctcgcca ccattatcgc cggaaaactg
    13801 gcccgtctgg ataacgtgct gcgcagtcgc tttggtgtag aagtggtcat tgaaccggaa
    13861 gtgacggacg aaatcatgag ccgcgtcacc cgcgcggaaa acggcgcgag gatgctggaa
    13921 tcggtcatcg atggcgacat gctaccgccg ctctcgctgc tgctgttgca gaaaatggcg
    13981 gctaacacgg cgattgcccg gattcggttg tcggcagtgg acggcgcatt tacggcagac
    14041 gtggaagatg ctcagaacga cgagtccgtc acaaaggatg aaacggtttt atgagcaggt
    14101 tgcgcgtttt ccggcagaca ggatggttta tcgcgggcct gatgacgggc ttacccgcca
    14161 ctgccgcgcc cgcagaagca tcgtcaatgg ccggtgtggc ggtcgccgtc gccaccacga
    14221 ccccgccgga tgcgacagcc acgttacagg ccatgcagtc ctgtcggcgg gagtccgcgg
    14281 cgctggagcg gcttgactgc tatgaccgcc tgctggcacc gctgtccccg tcagggtttg
    14341 acggggcgct ggtcaaagcc ggttttgtgg gcgaggcctg gacccgcgcc acagagcagg
    14401 aaaaacgccg cgagggcaac accacagaac tgctggtgac gcaggtgccg ggggagcgcc
    14461 cgacggtggt gataaccacc ccggctatcg gtcatgttcc gccacgcccg gtgctgatgt
    14521 tcagttgcgt ggacaatatc acccggatgc aggtggcgct gatgcaccct ctggatgtcc
    14581 atgatatcgc cgtcactctg aatgccgaca accgggcatt gcgcagccac tggtttgtgc
    14641 gcgagaacgg caccctgctg gagtccagcc gggggttatc cgggattgat gaaattaagc
    14701 aactgttcgg cgcaaaaacg ctgacggtgg ataccggcgc agacaatgcc gccggaaagc
    14761 tgacctttaa cattgacggg ctggcaaggg cgattgctcc cctgcgcgat gcctgccact
    14821 gggcgggaga ataaaaatat ggcaatggac ttacgcgatc cgaatgtctg gatatcgcac
    14881 ctgctggaaa acctgccgga agagaaactg gcatcggcgc tgaaagatga caacccgaac
    14941 tgggagtata tcgacggcga aatcgtcaag ctgggatcgc tggcccacag tcaactggat
    15001 atcccggaac tccagcgccg ggggctggtc attctggctt cggagagtaa ggattttcgt
    15061 ctgctggcgc atctgctaag aaccctgcaa cacgccggtg atcctcatct cgcattgcgc
    15121 ctgctggcgc tgtatgtgga gcattactgg gcggtggctg cgccgcagaa tatggcgcat
    15181 aaaaaacgct ttgccagcca ggtcattaaa cgctttgaaa caggtattga agttttttca
    15241 cagaacgccg ccacagcgca gcgcgatgcg ctgtcagggc aactggcaaa actggcgcaa
    15301 tgctggcagt cacataacgc cccggaactg gcacaggcta ctgatgatct ttttgccctg
    15361 taccagcgtg cgtttaatcg tgcggcaccg gctcctgtcc ccactccggc ggcctccggt
    15421 agttcaccac agaccaccgc cacgtctgaa agcggcgtga cgcaacccag tgctccggct
    15481 ccccaaatcg tcatcgacag tcacgacgac aaagcctggc gcgacacgct gttaaaagtg
    15541 gcggccattt tatgtgaacg ccagccggat tcaccgcagg gctaccgtct gcgccgccat
    15601 gcgctttggc agaacatcac cagtacgccc caggcggaaa gcgatggccg tactccactg
    15661 gctgcggtct ccgccgatat ggtggcagat taccatgctc agctcggtag tgccgatatg
    15721 gcgctgtggc agcaggtgga gaaaagcgta ctgctggctc cttactggct ggatggtcac
    15781 tgcctttctg cacagacggc gctgcgtctg ggttacaaac aggtggcaga cgccatccgc
    15841 gatgaggtca tccgcttcct tgagcgtctg ccccagctta ccgggctgct gtttaatgac
    15901 cacacaccgt ttatcagtga gcagacgaaa caatggctgg cagcttcgcc cgacgccaaa
    15961 gttgcaccgg ttgcgcaaat cggtgaggaa tcgaaggcag ccagagcctg ttttgctgag
    16021 cagggtctgg aggcggcgct gcgatatctg gacatgctac ccgaaggcga tccccgcgat
    16081 cagtttcacc gccagtacct tgccgcacag ttgacggaag aggcggggct ggtacagctt
    16141 gcgcagcagc agtaccggat gttgttcagg atgggattgc agatgatggt ggcagactgg
    16201 gagccgtccc tgcttgagca gctagaacaa aaattcacgg cagaacaata aaagagaaca
    16261 ggagtttttg tgttcagatt accaacaccc cgactactca gcggactcaa atcagccctg
    16321 cgaccggcga tgccccggtt taaagtctct gctttctggc tgctgatact ggcgtggatt
    16381 tttctgctgg tgtggatctg gtggaaaggc ccgatgtgga cgctgtatga agaacagtgg
    16441 ctcaaaccac tggctggcaa cggcggcgtg ggggattatt gccctggtgt ggcttaccgt
    16501 ccgggtgatg aagcgcctgc aacagcttga aaaaatgcaa aagcaacagc gcgaggaagc
    16561 cgttgatccg ctcagcgtgg aactgaacgc ccagcagcgt tatcttgacc gctggctgct
    16621 gcgcctgcaa cgccatctcg acaaccgccg tttcctgtgg cagttgccgt ggtatatggt
    16681 catcggcccg gcgggcagtg gcaaaacgac actgctgcgc aaggggtttc cgtccgacat
    16741 tatttatgcc ccggagggcg cacgcggcgc agaacaacgc ctgtacctca cgccccatgt
    16801 cggtaaacag gcggtgatct ttgatatcga cggcacactc tgcgctcccg ctgatgcgga
    16861 tatcctgcat cgccgtctgt gggaacatgc cctcggctgg ctgaaagaaa agcgcgcgcg
    16921 ccagccgctg aacgggatta ttctgacact cgatttaccc gatctgctga ccgcagacaa
    16981 acgccgccgc gagcatctgt tacagacgct gcgcagccgc ttacaggata tacgccagca
    17041 tcttcactgc cagttaccgg tttacgtggt actgacccgg cttgatttat tacagggttt
    17101 tgccgccctg ttccagtcac tcaacagaca ggatcgcgat gcgattctgg gcgtcacgtt
    17161 cacccgccgt gcccatgaaa atgatgactg gcgaacagag ttgaatgctt tctggcagac
    17221 atgggtggat cgaatgaatc tggcgttgcc ggatctgatg gtcgctcaga ctcacacccg
    17281 cgcgtcttta ttcagttttt cccgccagat gcagggaagc cgtgaaccgc tggtgtcact
    17341 gcttgagggt ctgcttgatg gcgaaaatat gaacgtgatg ctgcgtggtg tctatctcac
    17401 ctcttcgctt cagcgtggac agatggatga tatattcacc cagtctgccg cccgccagta
    17461 ccggctgggc aataacccac tggcgtcctg gcccctggtg gacaccgcgc cttatttcac
    17521 ccgcagcctg ttcccgcagg cattactcgc agagcctaat ctggcaacag agagccgcgc
    17581 ctggctgata cgttcccgtc gccgcctgac ggttttctct gccacaggcg gcgtggcagc
    17641 actgctgctc atcaccggct ggcatcacta ttacaacggt aactatcagt ccggcatcac
    17701 cgtgcttaag caggccaaag cctttatgga cgtgccgcct ccgcaggggg aagatgactt
    17761 tggcaacctg caactgccgc tcctgaaccc ggtacgcgat gccacactgg cctatggcga
    17821 ctggggcgac cgcagccgtc tggccgatat gggactgtac cagggacgac gtatcgggcc
    17881 ttatgtggaa cagacctatc tgcaactgct ggagcaacgt tacctgccct cgctgtttaa
    17941 cgggctggtc aaagcgatga acgccgcgcc gccggagagt gaagaaaaac tcgcggtgct
    18001 gcgcgtgatg cgaatgctgg aggacaaaag cggacgtaac aatcaggtgg tgaagcagta
    18061 tatggcaaaa cgctggagcg aaaagttcca cggtcagcgc gatatccagg cacaactgat
    18121 gtcccatctt gactacgcgc tggctcatac tgactggcac gcagagcgtc aggcgggcga
    18181 cggtgacgcc atcagccgct ggacgccata tgacaagccc gtggtatcag cacagaaaga
    18241 actgagcaaa ctgcctgtct accagcgggt ttaccagagc ctgaaaacgc gggcgctggg
    18301 cgttcttcct gccgacctca atctgcgtga ccaggtaggg ccaacctttg accaggtgtt
    18361 tacatctgcc gatgacaaca aactggttgt tccacagttt atcacccgct acggcctgca
    18421 aagctatttt gtaaaacagc gcgatgaact ggttgaactg acagcgatgg attcctgggt
    18481 acttaacctc acccgcaatg tgaaatacag tgacgccgac cgtgcggaaa tccagcacca
    18541 gttgaccgag cagtatatca gcgactacac cgccacctgg cgggctggga tggacaatct
    18601 gaatatccgc aattttgagt ccatcggaca actgaccggg gcgctggagc aggttatcag
    18661 cggcgaccag cctttgcagc gggcgctgac cgtgctgcgt gacagcacac agccaggcgt
    18721 cttttctgaa aaactctctg ccaaagaacg ggaggaagcc ctggcagagc cggattacca
    18781 gttactcacc cgcctcgggc atgaattcgc cccggaaaac agtaccctgg cagtacagaa
    18841 agacaaagaa agcacgatgc aggccgtgta tcagcaactc accgagttgc accgctacct
    18901 gctggcaatc cagaacgcgc ctgtaccagg gaaatcggcg ctgaaagccg tgcagttacg
    18961 gcttgatcag aacagcagcg atccgatatt cgccacccgc cagatggcaa aaacgctgcc
    19021 tgctccgctc aaccgctggg ttggcagact gactgactag gcctggcatg tggtgatggt
    19081 ggaggctgtt cattatatgg aagtggactg gcgcgacagc gtggtgaaac cgtttaacga
    19141 gcaactggca aataactatc cgtttaatcc gcgttctgca caggatgcct cactggatgc
    19201 cttcgaacgc ttctttaaac cggatggcat actggatacc ttctaccagc agaacctgaa
    19261 gctgtttatc gataatgacc tgagtctgga ggatggcgat aacaacgtca ttattcgcga
    19321 agatattatt gcgcaactgg aaactgcgca gaaaatccgt gacatcttct tcagcaaaca
    19381 gaacggtctg ggaacatcct ttgccgtgga aacggtatcg ctttcaggca ataaacgccg
    19441 cagtgtactg aaccttgaca gtcagttagt cgattacagc cagggccgta actataccgc
    19501 ccatctggtc tggcctaaca acatgcgcga aggcaacgaa agtaagctga cgctcatcgg
    19561 caccagcggc aacgcgccgc gcagtatcag cttcagcggg ccgtgggcgc agttccgcct
    19621 gttctgggcc ggacaactga ccggagtaca ggatggcaac tttaccgtgc gctttagcgt
    19681 ggacggtggc gcgatgacct accgtgtgca taccgacacg gaagataacc cgttcagcgg
    19741 tgggttgttc agccagtttg gtctgtcaga cacactgtac tgaggaggca gggatgaata
    19801 gtaacgtact gacacaaact atcgttaccg gcagtgaccc gcgcgggctg ccggaattca
    19861 gcgccatccg cgaggaaata aacaaagcca gccacccgtc acagcctgag ctgaactgga
    19921 aactggtgga gtcgctggcg ctggcgattt ttaaagccaa cggtgtggat ttacacaccg
    19981 ccacctacta tacgcttgcc cggacacgga cacagggact ggcgggattc tgcgaaggtg
    20041 cggaactgct ggcggcaatg gtaagccacg actgggataa gttctggccg cagggcggcc
    20101 cggcgcgtac tgaaatgctg gactggttta actcccgaac cggcaatatt ctgcgtcagc
    20161 aaatctcctt tgcggaatcc gacctaccac tgatctaccg cacagagcgg gcattgcagc
    20221 ttatctgcga taagctccag caggtggaac tgaagcgcgt tccgcgcgtg gagaatctgc
    20281 tctattttat gcagcacacg cgtaaacggc ttgaacccca gccgaagagt aacgctgaga
    20341 acgccgcaca gaccacggtc agaacgctga tttatgcccc ggaaacactg gcatcttcca
    20401 caccagaagc ggtagtgcct cccctgcccg gcctgcctga gatgaaagtg gaagtgcgca
    20461 gtctgacaga gaatccccca caggccagtg tgataaagca aggcagtacg gtaagagggt
    20521 ttatcgcagg gatcgcctgt tcagtggctg tcgcctcagc attgtggtgg tggcaggtct
    20581 atccggtgca gcagcaactg ttacaggtta acgacaccgc tcagggcgca gcaacggtgt
    20641 ggatggcctc acctgaactc gaaaactatg agcgcaggct gcaacaactt cttgatacct
    20701 ccccggtaca gccgctggaa accgggatgc agatgatgcg tgttgccgac agtcgctggc
    20761 cggaaagcct gcaacagcaa caggcctcga cacaatggaa tgaggcactc aaaacccgcg
    20821 cacagagtag cccgcagttg cgtggctggt tgcagacccg ccaggactta catgcttttg
    20881 cagatctagt gatgcagcgc gagaaagagg gactaaccct ttcctatatc aaaaatgtca
    20941 tctggcaggc ggagcgggga ctggggcagg aaacccccgt tgagtctctg ttgacgcagt
    21001 accatgatgc ccgtgcgcag aagcagaata cagatgcgct ggaaaaacaa attaatgagc
    21061 gactcgaagg cgtgttaagc cgctggctgc tgctgaagaa taacgtcatg ccagaggcgg
    21121 caaccggtac cacggccgaa aaataattat ttaattaaag gagattatca tggcaaatat
    21181 aagttattta tctttgagtg gagagacaca agggctaatt tcagcaggtt gctcaacgct
    21241 tgattctgtc ggaaacaaag cgcagccaga acataaagat cagatcatgg tttatgcgtt
    21301 aatgcattcc atctcaagaa gtcaaaatgt caatcatcat gaattaatta tcaccaaacc
    21361 ggttgataaa tcctcaccat tgctggcaaa agcacttagt gacaatgaaa aaatggcaat
    21421 atgtgaattt atactctacc gaaccagtaa agcgggtatt taccagcctt attataaaat
    21481 aaatctgtca aaagcccgaa tatcaagtat tgattttgtc acaccgcatg ctgtcctgga
    21541 aaaagaactg gaaccacagg agagaattgc ctttatttat gaagatattt cctgggagca
    21601 tacgttagcc ggtacgaatg caatgagtaa atggcaggat cgtgtacagt aacttactgt
    21661 aataacataa taaattacta ttgcggagag ataaaatggc tggtttaagg aataacaaca
    21721 acacacagaa tgcacagtgg gctgattatg tgggtgacat tcttcggggg gctcagccga
    21781 taaaccagct agtacctcag cacccttatc ttaacgacgt cccactcatt gatgagttaa
    21841 ggcatcagaa cactcatcat gttattctct tgacacttga tgttgcaaaa aaaaaaaaaa
    21901 aaaaaaaaaa aaaaaaaaaa aaaaaaatac tcagcccaat aacatcattt gattacattc
    21961 attttatcac tacacatcct tcaggtataa aagatacact cgcctggtta gttaacgcga
    22021 gtaaattaat gactgagttt gatgacaatg ggaaaattat atttaacatt aatgccttaa
    22081 agtatacaaa agcaagttac tttgaaatac ttggagagaa atatataaaa atcacaacat
    22141 ccagcccctg gcttttagaa aagctcggta aatatatttt ctcttcacga gcgcctcaag
    22201 ttctggaact cgctattggc tggcgaggag ctttatctga atcaataaaa ggagttaagt
    22261 tctgtatctg gttttcagta gcctggagaa ccatagaatt tattatgagt tcagagcgag
    22321 atctggttaa ttttcttggc gatttttcaa tggatgtggc caaagcagtt atagccggtg
    22381 gtgttgcaac cgctattgga agcctggctt cttttgcctg tgttagcttt ggctttccag
    22441 taattcttgt cggaggagca attttactga cagggatagt gtgtacagtt gttttaaatg
    22501 aaatcgatgc tcaatgccat ttatcagaaa aattaaaata tgcaattaga gatggactaa
    22561 aacggcaaca ggaacttgat aaatggaaaa gggaaaacat gactccattt atgtatgttc
    22621 ttaacactcc acccgtgata tgaggattaa ccatgcgttt acatgttaaa ttaaaagagt
    22681 ttctttcgat gttttttatg gccatattat ttttccctgc attcaatgct tctttgtttt
    22741 ttaccggagt taaacctcta tattcaataa taaaatgctc cacggaaata ttttatgatt
    22801 ggagaatgtt gattctgtgc tttggtttca tgtcgttttc ctttttaaat attcatgtaa
    22861 ttttacttac gataataaaa ttattcttaa tcaaaaaaac gaaggtagtc aatttcgcca
    22921 cagacataac aatacagcta actcttatat ttttactaat agctatcgta atagcacctt
    22981 taatagctcc ttttgtcaca ggatatgtca atgctaatta tcacccctgc ggtaataata
    23041 cgggaatttt cccaggagca atctatatta aaaatggcat gaaatgtaat aatgaatata
    23101 tttctcgcaa ggaagattca gcagttaaat aattaaacat tctaaaacaa acagttttta
    23161 actccggatt ccatgtcctt tcttgcactg tctttgtcaa gaaaaatttc ttatgcaaag
    23221 aattttgtat tatttccgcc agaaattcct ggcggcagat ataatttatt attgatagcg
    23281 gttaaccaaa tcttttacca tcgctttata ggtcttttgc ttaagttgca tgatttgctg
    23341 ctctgtcata tcagtcgtac tggcttcatt cttgtcgggc aatggtggaa gcactttata
    23401 ttcataattc accggcgacc aggcagccca ctcacctgta gctacatcca ccagcgtgaa
    23461 tcgtaccaga tagcgcagtg acatcgtgtc agtcaatttt tgatttttat aatctgacca
    23521 aactattgat ttcaatccag agtcatattt tcctgtctgc aacgtatcct ggaaaacaat
    23581 aatcgctttc tgatgccctt ttgcagccac aaaacgcagt gcctgcatcc agttcatatt
    23641 ctcagcactg gcaacatcct cgttctcatt tttatctttg tttttgttac aagtcagagg
    23701 cttttgcctg tccgggatac cagagaatgt ggaaacagta taatattttc gcatctcctc
    23761 ctgcataatg gtttccggtg cgcggttgcc ggattgaaca agtattacag gagaattaag
    23821 gggaacgcgg aactcatctc catcaagcgc agcttgaata tcctcttcgg atacggccag
    23881 agtggtttca ttaccgaata aatctttgtc tgtcagttgc tggggagtgt ttttattaat
    23941 attactttgt gtgacttgag cttttttctt cttttcatca atagaagatt cagaggcaca
    24001 tcctgcaagc agcgagaata atacacatat acaacaaacc cttcttaaat tcataatcgg
    24061 acactccctc gcctgtcata atcatggtaa tgtttagcta acgattttta attatatact
    24121 ccacttgaat cagaacaaac aaaaataaca gtatatataa attcattaaa tcaatagaaa
    24181 aatgaaaatc ataacattca aatatattta aattgaatga ttcgatagta tgattaaata
    24241 ttaatgatat ggtctaccat atgtgattaa cacatttaca gcataacaaa accatagacc
    24301 gaagaaatag cgggatttaa atgcaaaaaa accgcaaaat taaaaatctt gcggttctct
    24361 gaactcattt tcatgagtga atttggcgga acggacggga ctcgaacccg cgcccccctg
    24421 cgtgacaggc aggtattcta accgactgaa ctaccgctcc gcgttgtgtt ccgttgggaa
    24481 cgaggcgaat attacggatt gcctcaccct tcgtcaacgg tttttctcat cttttgaatc
    24541 gtttgctgca aaaatcgccc aagccgctat ttttagcgcc ttttacaggt atttatgccc
    24601 gccagaggca gcttccgccc ttcttctcca ccagatcaag acgggcttca tgagctgcaa
    24661 gctcttcatc tgtcgcaaaa acaacgcgta acttacttgc ctgacgtaca atgcgctgaa
    24721 ttgttgcttc accttgttgc tgctgtgtct ctccttccat cgcaaaagcc atcgacgttt
    24781 gaccaccggt catcgccaga taaacttccg caaggatctg tgcatcgagt aatgccccgt
    24841 gtagcgttcg tttactgtta tctatttcgt agcgggcaca taacgcatcg aggctgttgc
    24901 gcttaccagg aaacattttc ctcgccaccg caaggctatc ggtgacctta cagaaggtat
    24961 tggtcttcgg aatatcgcgc ttaagcaacg aaaactcgta gtccataaag ccgatatcga
    25021 acgctgcgtt atggatcacc aactccgcgc cgcggatata gtccatgaac tcatcggcta
    25081 cttcggcaaa cgtgggctta tcgagcaaaa attcatcggc aataccatgt acgccaaagg
    25141 cttccggatc caccagccga tcgggtttga gataaacatg gaagttattg cccgtcaggc
    25201 gacggttcac cacttcaacg gcaccaatct caatgatctt gtggccttca tagtgcgcac
    25261 caatctggtt cataccggtg gtttcggtat cgagaacgat ctggcgtgta attgcagtgc
    25321 tcatagcggt catttatgtc agacttatcg ttttacagtt cgattcaatt acaggaagtc
    25381 taccagagat gcttaaacag gtagaaattt tcaccgatgg ttcgtgtctg ggcaatccag
    25441 gacctggggg ttacggcgct attttacgct atcgcggacg cgagaaaacc tttagcgcag
    25501 gctacacccg caccaccaac aaccgtatgg agttgatggc cgctattgtc gcgctggagg
    25561 cgttaaaaga acattgcgaa gtcattttga gtaccgacag ccagtatgtc cgccagggta
    25621 tcacccagtg gatccataac tggaaaaaac gtggctggaa aaccgcagac aaaaaaccag
    25681 taaaaaatgt cgatctctgg caacgtcttg atgctgccct agggcaacat caaatcaaat
    25741 gggaatgggt taaaggccat gccggacacc cggaaaacga acgctgtgat gaactggctc
    25801 gtgctgcggc aatgaatccc acactggaag atacgggcta ccaagtcgaa gtttaagctt
    25861 gtggtttacg acattgccgg gtggctccaa ccgcctggcg aattcgtggc ttgtttttac
    25921 tctgtttcat cgggtttagc gttaaaggaa tagtccgttt ccgggcaaca ataagttgta
    25981 agcaaccaag cgcaggaata tgcgcattca atagttttcc tccgtgtttg ttccacggga
    26041 gaacgtggaa acggctggcg tgcagcactt caaaattcaa caaagagagc cagtccagtt
    26101 gccgcatcag agtaaacatc cggctgttat agggcgaggt ttttcgcaat accggcacaa
    26161 gtttgcgtaa tcccattaaa ctaatgggat tgaagccact aatcaccagc cagccatcat
    26221 caatcaatac ccgatcggtt tcacgcaata aacgatgcgg atcggtgcac cacggcaatg
    26281 tatgtgccag tagacaaaca tcaacggatt tatcggcaaa aggaagatga agtggatccg
    26341 cctgcacctg gacgggcatt ccttgcgcag aaacattcac ttgatgagaa accgcgcacg
    26401 cttcgcaatt gatttctgcg cttaaattgc caatcttaag cagatgaaaa ccatacattt
    26461 tagtgaacca cgggttgagc tggcgctcca gcgccttgcg ataaagctct ccccagggca
    26521 aatcgcccca gcgatcagga gccacgacag tttgagggac tcttgccggt ttcatcacaa
    26581 ccttccgttt cacactaaga ggtaatctat gaatcttaac agtattcccg cctttgatga
    26641 caattacatc tgggttttga atgatgaagc aggtcgctgc ctgattgtcg atcccggaga
    26701 cgcagagcca gtattaaacg ccattgccgc caataactgg caaccggagg ccatatttct
    26761 cacccaccat catcacgatc acgttggcgg cgtaaaagaa ctggtggaaa agtttccgca
    26821 aattgtggtg tatggtccac aagagacaca agataaggga acaacacagg tagtcaaaga
    26881 tggcgaaact gccttcgttt tggggcatga atttagtgta attgccacac cgggtcacac
    26941 tttaggacat atctgttact tcagtaaacc ttatctattt tgcggcgaca cgctgttttc
    27001 tggtgggtgt ggtaggttgt ttgaagggac agcatcacaa atgtatcaat cacttaataa
    27061 gttaagtgcg ttacctgacg atacattggt atgttgtgct catgaatata cgttatcaaa
    27121 tatgaagttt gctttgagta ttcttccgca cgatttgtcc ataaatgatt attatcgtaa
    27181 agttaaggag ttacgggcaa aaaatcaaat aacactaccc gtaattctga aaaatgagcg
    27241 gcaaattaat gtttttttaa gaacggaaga tattgattta attaatgtaa ttaatgaaga
    27301 aacattattg caacaacctg aagagcgttt tgcatggtta aggtcaaaga aagataggtt
    27361 ctgataaaac tttcttgtca tcggctccgt tcgccgttat gatcggtcgt cttttaagca
    27421 actattgaca cacacatgaa ggcaaaagcg atattactcg cctctgtcct gctcgtgggt
    27481 tgccagagta ccggcaacgt tcaacagcac gcacagagcc tttctgcagc tggtcaaggg
    27541 gaagcagcaa agtttacaag tcaggcacga tggatggacg atgggacgtc tatcgcgcca
    27601 gatggtgact tgtgggcttt cattggcgac gagctaaaga tgggaattcc ggaaaatgac
    27661 cggattcgcg aacagaaaca gaaatattta cgcaataaga gctatctcca cgatgtaact
    27721 ttacgggcag agccgtatat gtactggata gcagggcaag ttaaaaaacg taacatgcct
    27781 atggaactgg tactactacc catagtggag agcgcttttg atcctcacgc aacgtctggc
    27841 gccaatgccg caggcatctg gcagatcatt ccgagcacgg ggcgcaatta tggtttgaaa
    27901 cagacccgca attatgacgc gcgtcgcgat gttgttgctt caacaactgc cgcgctgaat
    27961 atgatgcagc gtctgaacaa aatgtttgac ggcgactggc ttctgaccgt agcggcttat
    28021 aacagcggcg aaggtcgggt catgaaggca attaaaacga acaaagcgcg tgggaaatcc
    28081 acggacttct ggtcgttacc gttgccgcag gaaacgaagc agtacgtgcc taaaatgctg
    28141 gcattgagtg atattctcaa aaacagcaag cgttatggcg tacgtctgcc aacgaccgat
    28201 gaaagccgtg ctctggcgcg tgtgcacctg agtagcccgg ttgaaatggc gaaggttgca
    28261 gatatggcgg ggatttccgt cagcaagctg aagacattca acgctggcgt gaaaggctcc
    28321 acgctgggcg caagtgggcc gcagtacgtg atggtgccaa agaagcatgc agatcaactg
    28381 cgtgaatctc tcgcttcagg cgaaattgct gctgtacagt cgacgctggt tgccgacaat
    28441 acgccgctta acagccgtgt ttacaccgta cgctctggcg acacgctttc aagcatcgct
    28501 tcacgtctcg gcgtaagcac caaagatttg cagcagtgga acaaactgcg cggatctaag
    28561 ctgaagccag gccaaagttt gacgattggt gcaggcagta gcgcacagcg gttggcaaac
    28621 aacagcgata gcattacgta tcgtgtgcgc aaaggcgatt cgctttcaag cattgctaaa
    28681 cgccacggcg tgaacatcaa agatgtgatg cgctggaaca gcgacactgc gaatctgcaa
    28741 ccaggcgata agctgacgtt gtttgtgaaa aacaacaaca tgccagattc ctgacaaacc
    28801 agataataaa aaaggcaccg attcccccgg tgcctttttt atttatgccg ctttatgtgc
    28861 ttctaccatg atgatatcac tggtgaaaga gccatcagtc tgcaaggaaa aatacgttct
    28921 cacctctgtc gatgcgctct gttggtaaat gcgaatagcg tctactaacg cttctggcgt
    28981 acgcattctc acgacccatg aagaaaattc cagcggtaac ttatctgtaa ttaaattatc
    29041 aactatcaga ttggcttcat tgattaacgt caaccacgca ccgctggcgt agtttcgtac
    29101 gtgagaggta tcgcgtaatg cttctaccgc ctgtaaccag atgtcgcgca ctgggtgacc
    29161 cggagacatt acatccatca caatcagcct accgccaggt ttcaatatcc tattcacttc
    29221 tcgcagtgct gcaccaacat catgccagtg atgggcagaa taacggctga taacaatatc
    29281 aaatgcgtta tcggcaaatg gcagacttgc ggcatatccc tggcgggtgg tgatattttt
    29341 cagttgccgg gcttcggcag cttgtgccac gacatccagc atgtgggcag ataagtcata
    29401 cgccaccacc gcgctcacgt tttgcgcagc gccaaacgtt ggtctgccct gcggcagacc
    29461 tgacatacct tatttatcag gcttgccggg actgaattca acgagtagcg gattgtgatc
    29521 ggaagcgcgc gtaaccagta cagaagcttc actgacgttc agaccacggt agaaaacaaa
    29581 atcgagcggg cgaccaaacg cccgacggcg ctgatcatcg gtaaaacgca cctggcgcag
    29641 cgacatttcc cgcgcaaagc gatataacgc gttcatcctt ctacggctcc aggcattgaa
    29701 atctcccgcc ataatgaccg ggccgctgtg gtgagctatc tgatcaccaa taggaagtaa
    29761 ctgcttacta tagacatcca cgcccagact gaagttgacg gcgtgtatat taaccaccat
    29821 caacaggcgg gtgtcaggta atggatagac cgtcaccagt gccgacttcg ccagacgcaa
    29881 aatgggttct cgttcacgta acgggcagca atacactgga tgtgccgccg aaagggtcat
    29941 tacgccagaa ggatgttgtg gcagcacgaa agcgggtacc tgatcggcgg caagatagtt
    30001 agcggtcgca aactgtacca actctggcgt tgtttgcgct tcctgcaata acaccagatg
    30061 tgcgtctttg ccgtaattct ttaataccga caaccattca gcgcgttgct gtttgtatat
    30121 gttccacacc aggatccgaa tacgctcttc ggtacttaac ggttctccgg gtggtaatgc
    30181 ctggccgatg ctcgcaaaag accccggcgg taagatcctc tccgcaggtt gtccggcaac
    30241 atagcgcatg gcataggtgt tttttcgcac ttttgacttt gaaacctcta ttacgtcaac
    30301 ccttccgcac tcgggagggt tctactgtta tagggatttt agctcactct ttcaacgagc
    30361 aattgtatat tgttatgtaa gcaagtgctt acaccggtca gaaatttgct cattattgag
    30421 aacattaaat cggatgacat atcacgcatc ctgatggttt aatgacaaat aaattcaaaa
    30481 tatctcatct attttgcaac aggagcaaaa atgaaagcca cgtcggaaga actcgccatt
    30541 tttgtttcgg tcgtcgaaag cggcagcttt agccgggcag cggaacaatt agggcaagca
    30601 aactcagcgg taagccgggc ggtgaaaaag ctggaaatga aacttggcgt tagcctgctt
    30661 aatcggacca cgcggcaact tagcctgacg gaagaaggcg agcgttattt tcgtcgcgta
    30721 cagtcaattt tgcaggagat ggcagcggca gaatcagaaa ttatggagac gcgtaataca
    30781 ccgcgtggac tgttacggat cgatgccgca actccagtgg tgctgcactt tctgatgccg
    30841 ttaattaagc ctttccgtga acgctatccg gaagtcactt tatcgctagt ctcctccgaa
    30901 acgattatta atttgatcga aagaaaagtg gatgtcgcga tacgcgctgg tacgttaacg
    30961 gactccagct tacgtgccag gccgttattt aacagttatc gaaaaattat cgcctccccc
    31021 gattatattt cccgctacgg gaagccagaa acgatcgacg atttaaagca acatgtttgc
    31081 ctgggattca ctgaacccgc ttccctcaat acctggccga tagcctgtag tgatggacaa
    31141 ttacatgagg tgaagtacgg tttgtcatcc aatagtgggg aaacactgaa acagctttgc
    31201 ctgagtggca acgggattgc gtgtttgtcc gactacatga tcgacaaaga aatcgctcgc
    31261 ggagaattgg tggagttaat ggcagataaa gtgttgccag tggaaatgcc attcagtgcc
    31321 gtctattaca gcgaccgtgc ggtaagtacg cgcatccggg cttttatcga tttccttagc
    31381 gagcatgtaa aaacagctcc cggaggagct gtcagagagg cttaatccca ttcaggagcc
    31441 agaccttccg ggctaaccag gcggtcgttg caatccagtg cggcgatcgc ttttttatct
    31501 tcggcatcaa gctgtaaatt ttgtgcctta agattacttt ccaggttttt acgtttagta
    31561 gaagaaggaa ttactgagta accttccccc atagcccacg ccagaatcac ttgtgccgga
    31621 gtcgcattgt gtttagctgc aatacgagca ataacctcat ctttcagtgc cttaccataa
    31681 gccagcgtca tataggaagt gatatgaata ccgtgctgtt tagcccaggc aaccacttta
    31741 cggttttgca gataaggaga gagttcaatc tggttggtag cgatgttttc agcgccaaca
    31801 gcagcaatcg ctttttccat caacgggatc gtgaagttgg aaataccgat ctcacgtgtc
    31861 agaccttgtt ttttggcctc cagcagcgcc tgcataaact cttcaacaga gacttcatcg
    31921 tttggtgacg gccagtggat tagcgtcaga tcaacataat cggtacgcaa tttttgcagg
    31981 ctctctttta gactcgggat caatttgtct ttgctgagat tttcaatcca gattttagtg
    32041 gtgatgtaga gttcatgacg tggcacgcca ctttctgcaa tcgcctgacc tactgcggct
    32101 tcgttatcat agatttgtgc agtatcaatt gcgcgataac caagttcaag cgccgttttc
    32161 acagatgaaa taacaacgtc gtctttgaga cggaaagtac ctaaaccaaa tgcagggata
    32221 gccataatat gcctctttta atgctatttt cgttaactga ggtgattatg ggcttcagac
    32281 tgttgcggaa aaagagcata aaatgcagag gatttttgcg attctggcaa taatagatat
    32341 acagacgaaa aaaaacctcg agcgttaact caaggcttct taataagtgg cggaacggac
    32401 gggactcgaa cccgcgaccc cctgcgtgac aggcaggtat tctaaccgac tgaactaccg
    32461 ctccaccgaa ttcttttaca accaccggtt ttatgaccgg cttactgctt aatttgatgc
    32521 ctggcagttt atgaatcact tcgtgattca cccttcgggc cgttgcttcg caacgttcaa
    32581 aagctttcgc ttttgtccta ctctcttatc gagtagtgaa ctgcctcaaa ggtcagtgcc
    32641 attcttc
//
LOCUS       DAESHI010000041        31118 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.041, whole genome shotgun sequence.
ACCESSION   DAESHI010000041 DAESHI010000000
VERSION     DAESHI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31118
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.041"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            19..714
                     /gene="araD"
                     /locus_tag="KW011_001948"
     CDS             19..714
                     /gene="araD"
                     /locus_tag="KW011_001948"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBH7660283.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            789..3140
                     /gene="polB"
                     /locus_tag="KW011_001949"
     CDS             789..3140
                     /gene="polB"
                     /locus_tag="KW011_001949"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HBH7660284.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            3304..6210
                     /gene="rapA"
                     /locus_tag="KW011_001950"
     CDS             3304..6210
                     /gene="rapA"
                     /locus_tag="KW011_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HBH7660285.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            6222..6881
                     /gene="rluA"
                     /locus_tag="KW011_001951"
     CDS             6222..6881
                     /gene="rluA"
                     /locus_tag="KW011_001951"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HBH7660286.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(6998..7813)
                     /gene="djlA"
                     /locus_tag="KW011_001952"
     CDS             complement(6998..7813)
                     /gene="djlA"
                     /locus_tag="KW011_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HBH7660287.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            8068..10422
                     /gene="lptD"
                     /locus_tag="KW011_001953"
     CDS             8068..10422
                     /gene="lptD"
                     /locus_tag="KW011_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HBH7660288.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            10475..11761
                     /gene="surA"
                     /locus_tag="KW011_001954"
     CDS             10475..11761
                     /gene="surA"
                     /locus_tag="KW011_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HBH7660289.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            11761..12750
                     /gene="pdxA"
                     /locus_tag="KW011_001955"
     CDS             11761..12750
                     /gene="pdxA"
                     /locus_tag="KW011_001955"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HBH7660290.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            12747..13568
                     /gene="rsmA"
                     /locus_tag="KW011_001956"
     CDS             12747..13568
                     /gene="rsmA"
                     /locus_tag="KW011_001956"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBH7660291.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            13571..13948
                     /gene="apaG"
                     /locus_tag="KW011_001957"
     CDS             13571..13948
                     /gene="apaG"
                     /locus_tag="KW011_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HBH7660292.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            13955..14797
                     /gene="apaH"
                     /locus_tag="KW011_001958"
     CDS             13955..14797
                     /gene="apaH"
                     /locus_tag="KW011_001958"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HBH7660293.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(14875..15354)
                     /gene="folA"
                     /locus_tag="KW011_001959"
     CDS             complement(14875..15354)
                     /gene="folA"
                     /locus_tag="KW011_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HBH7660294.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(15546..17408)
                     /gene="kefC"
                     /locus_tag="KW011_001960"
     CDS             complement(15546..17408)
                     /gene="kefC"
                     /locus_tag="KW011_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HBH7660295.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(17401..17931)
                     /gene="kefF"
                     /locus_tag="KW011_001961"
     CDS             complement(17401..17931)
                     /gene="kefF"
                     /locus_tag="KW011_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HBH7660296.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(18039..19370)
                     /locus_tag="KW011_001962"
                     /pseudo
     CDS             complement(18039..19370)
                     /locus_tag="KW011_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(19429..19716)
                     /gene="fixX"
                     /locus_tag="KW011_001963"
     CDS             complement(19429..19716)
                     /gene="fixX"
                     /locus_tag="KW011_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HBH7660297.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(19713..20999)
                     /locus_tag="KW011_001964"
     CDS             complement(19713..20999)
                     /locus_tag="KW011_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBH7660298.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(21050..21991)
                     /locus_tag="KW011_001965"
     CDS             complement(21050..21991)
                     /locus_tag="KW011_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBH7660299.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(22006..22776)
                     /locus_tag="KW011_001966"
     CDS             complement(22006..22776)
                     /locus_tag="KW011_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HBH7660300.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            23249..24763
                     /gene="caiT"
                     /locus_tag="KW011_001967"
     CDS             23249..24763
                     /gene="caiT"
                     /locus_tag="KW011_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HBH7660301.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            24794..25936
                     /gene="caiA"
                     /locus_tag="KW011_001968"
     CDS             24794..25936
                     /gene="caiA"
                     /locus_tag="KW011_001968"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HBH7660302.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            26065..27282
                     /gene="caiB"
                     /locus_tag="KW011_001969"
                     /pseudo
     CDS             26065..27282
                     /gene="caiB"
                     /locus_tag="KW011_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
     gene            27356..28909
                     /gene="caiC"
                     /locus_tag="KW011_001970"
     CDS             27356..28909
                     /gene="caiC"
                     /locus_tag="KW011_001970"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HBH7660303.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MAQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            29018..29803
                     /gene="caiD"
                     /locus_tag="KW011_001971"
     CDS             29018..29803
                     /gene="caiD"
                     /locus_tag="KW011_001971"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HBH7660304.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            29809..30399
                     /gene="caiE"
                     /locus_tag="KW011_001972"
     CDS             29809..30399
                     /gene="caiE"
                     /locus_tag="KW011_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HBH7660305.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(30608..31003)
                     /gene="caiF"
                     /locus_tag="KW011_001973"
     CDS             complement(30608..31003)
                     /gene="caiF"
                     /locus_tag="KW011_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HBH7660306.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
BASE COUNT         7543 a         8375 c         8151 g         7049 t
ORIGIN      
        1 gacctctcag gagtcaacat gttagaagat ctcaaacgcc aggtattaga agccaacctg
       61 gcgctgccaa aacacaacct ggtcacgctc acatggggca acgtcagcgc cgttgatcgc
      121 gagcgcggcg tctttgtgat caaaccttcc ggcgtcgatt acagcgtcat gaccgctgac
      181 gatatggtcg tggttagcat cgaaaccggt gaagtggttg aaggtacgaa aaagccctcc
      241 tccgacacgc caactcaccg gctgctctat caggcattcc cctccattgg cggcattgtg
      301 catacgcact cgcgccacgc caccatctgg gcgcagtcgg gtcagtcgat tccagcaacc
      361 ggcaccaccc acgccgacta tttctacggc accattccct gcacccgcaa aatgaccgac
      421 gcagaaatca acggcgaata tgagtgggaa accggtaacg tcatcgtaga aacctttgaa
      481 aaacagggta tcgatgcagc gcaaatgccc ggcgttctgg tccattccca cggcccgttt
      541 gcatggggca aaaatgccga agatgcggtg cataacgcca tcgtgctgga agaggtcgct
      601 tatatgggga tattctgccg tcagttagcg ccgcagttac cggatatgca gcaaacgctg
      661 ctggataaac actatctgcg taagcatggc gcgaaggcat attacgggca gtaatgactg
      721 tataaaacca cagccaatca aacgaaacca ggctatactc aagcctggtt ttttgatgga
      781 ttttcagcgt ggcgcaggca ggttttatct taacccgaca ctggcgggac accccgcaag
      841 ggacagaagt ctccttctgg ctggcgacgg acaacgggcc gttgcaggtt acgcttgcac
      901 cgcaagagtc cgtggcgttt attcccgccg atcaggttcc ccgcgctcag catattttgc
      961 agggtaaaca aggctttcgc ctgacaccgc tggcgttaaa ggattttcac cgccagccgg
     1021 tgtatggcct ttactgtcgc gcccatcgcc aattgatgaa ttacgaaaag cgcctgcgtg
     1081 aaggtggcgt taccgtctac gaggccgatg tgcgcccgcc agaacgctat ctgatggagc
     1141 ggtttatcac ctcaccggtg tgggtcgagg gtgatatgca caatggcact atcgttaatg
     1201 cccgtctgaa accgcatccc gactatcgtc cgccgctcaa gtgggtttct atagatattg
     1261 aaaccacccg ccacggtgag ctgtactgca tcggcctgga aggctgcggg cagcgcatcg
     1321 tttatatgct ggggccggag aatggcgacg cctcctcgct tgatttcgaa ctggaatacg
     1381 tcgccagccg cccgcagttg ctggaaaaac tcaacgcctg gtttgccaac tacgatcctg
     1441 atgtgatcat cggttggaac gtggtgcagt tcgatctgcg aatgctgcaa aaacatgccg
     1501 agcgttaccg tcttccgctg cgtcttgggc gcgataatag cgagctggag tggcgcgagc
     1561 acggctttaa aaacggcgtc ttttttgccc aggctaaagg tcggctaatt atcgacggta
     1621 tcgaggcgct gaaatccgcg ttctggaatt tctcttcatt ctcgctggaa actgtcgctc
     1681 aggagctatt aggcgaagga aaatctatcg ataacccgtg ggatcgaatg gacgaaattg
     1741 accgccgttt cgccgaagat aaacctgcgc tggcaactta taacctgaaa gattgcgagc
     1801 tggtgacgca gatcttccac aaaactgaaa tcatgccatt tttactcgaa cgagcaacgg
     1861 tgaacggcct gccggtggat cggcacggcg gttcggtggc agcgtttggt catctctatt
     1921 ttccgcgaat gcatcgcgct ggttatgtcg cgcctaatct cggcgaagtg ccgccgcaca
     1981 ccagccctgg cggctacgtg atggattcac ggccagggct ttatgattca gtgctggtgc
     2041 tggactataa aagcctgtac ccgtcgatca tccgcacctt tctgattgat cccgtcgggc
     2101 tggttgaagg catggcgcag cctgatccag agcacagtac cgaaggtttt ctcgatgcct
     2161 ggttctcacg ggagaaacat tgcctgccgg agattgtgac taacatctgg cacgggcgcg
     2221 atgaagccaa acgtcagggt aataaaccac tttcgcaggc gctgaagatc attatgaatg
     2281 ccttttatgg cgtgcttggc accaccgcct gccgcttttt cgatccgcgc ctggcatcgt
     2341 cgatcaccat gcgtggtcat cagatcatgc ggcaaaccaa agcattgatt gaagcgcagg
     2401 gctacgacgt tatctacgga gataccgact cgacttttgt ctggctaaaa ggtgcacatt
     2461 cggaagaaga ggcggcgaaa atcggtcgtg cactggtgca gcacgttaac gcctggtggg
     2521 cggaaacgct gcaaaaacaa cggctgacca gcgcattaga actggagtat gaaactcatt
     2581 tctgccgttt tcttatgcca accattcgcg gagctgatac cggcagtaaa aagcgctacg
     2641 ccgggctgat tcaggagggc gataaacagc ggatggtgtt taaagggctg gaaaccgtgc
     2701 gcaccgactg gacgccgctg gcccagcagt ttcagcagga gctatacctg cgcatcttcc
     2761 gcaacgagcc atatcaggaa tatgtacgcg aaaccatcga caaactgatg gcgggtgaac
     2821 tggatgcgcg actggtttac cgtaaacgcc ttcgccgtcc gctgagcgag tatcagcgta
     2881 atgtgccgcc tcatgtacgc gccgctcgcc ttgccgatga agaaaaccaa aagcgtggtc
     2941 gccccttgca atatcagaat cgcggcacca ttaagtacgt atggaccacc aacggcccgg
     3001 agccgctgga ctaccaacgt tcaccactgg attacgaaca ctatctgacc cgccagctac
     3061 aacccgtggc ggagggaata ctccctttta ttgaggataa ttttgctaca cttatgaccg
     3121 ggcaacttgg gctattttga gcaaaaaaag agttcgccag ataccatttt gatgcgtgac
     3181 gaatgctttg ccatccagta ccatagcgcc ctttccattc ctggacctga ataacaccac
     3241 taccccataa gcacggtagc gggtggttat tgcctgcaat taaagatata gagccgaaca
     3301 catatgcctt ttacacttgg tcaacgctgg atcagcgata cagaaagcga attgggactt
     3361 ggaaccgttg tcgcggtgga tgcgcgaact gtcactttac ttttcccatc tactggtgaa
     3421 aaccgtctgt acgcacgcag tgattccccc gtgacccgcg tgatgttcaa ccctggtgat
     3481 actattacca gccatgacgg ctggcagatg caagtcgaag aagtaaaaga agaaaatggc
     3541 ttgctgacct atatcggtac tcgcctggat actgaagagt ccggcgtagc cctgcgtgaa
     3601 gttttccttg atagcaaact ggtgttcagc aaaccgcagg accgtctgtt tgccgggcag
     3661 attgaccgta tggaccgctt tgcgctgcgt tatcgcgcgc gtaagtattc cagcgaacag
     3721 ttccgtatgc cgtacagcgg cctgcgcggt cagcgtacca gcctgatccc gcatcagctc
     3781 aacatcgctc atgatgttgg tcgccgccac gcgccgcgcg tcctgctggc tgacgaagtg
     3841 ggtttaggga aaaccattga agccgggatg atcctgcatc agcaactgct ctctggcgct
     3901 gctgaacgtg tgctaattat cgtcccggaa accttacagc atcagtggct ggtagaaatg
     3961 ctgcgccgtt tcaacctgcg ctttgcgcta tttgatgatg agcgttatgc cgaagctcag
     4021 cacgatgctt acaacccgtt cgacaccgaa cagctggtga tttgctcgct ggattttgcc
     4081 cgtcgtagca aacagcgcct ggaacatctc tgtgaagccg aatgggacct gctggtggtc
     4141 gatgaagcgc atcacctggt gtggagcgaa gatgcgccaa gccgtgaata tcaggccatt
     4201 gaacaactgg cagagcacgt gccgggcgtt ctgctgctga ccgcgacccc ggaacagctg
     4261 gggatggaaa gccacttcgc ccgtctgcgt ctgctggacc cgaaccgttt ccacgatttt
     4321 gcgcagttcg ttgaagagca gaaaaattat cgtccggttg cggacgccgt tgccatgctg
     4381 ctggcaggta acaaactgag caatgacgaa ctgaacatgc tcggcgagat gatcggcgag
     4441 caggatatcg agccgctgtt gcaggcagca aacagcgaca gcgaagatgc ccagagcgcc
     4501 cgtcaggagc tggtttcgat gctgatggat cgccacggca ccagccgcgt gctgttccgt
     4561 aacacgcgta acggtgtgaa aggattcccg aaacgcgagc tgcacaccat taagctgccg
     4621 ctgccgacgc agtatcagac ggctattaaa gtctccggca ttatgggcgc acgtaaaagt
     4681 gcggaagatc gtgctcgcga tatgctctac ccggagcgta tttatcagga atttgaaggt
     4741 gataacgcca cctggtggaa cttcgatccg cgcgttgagt ggctgatggg ctacctgacc
     4801 agccatcgct ctcagaaagt gctggtgatc tgcgccaaag ctgccactgc gctgcaactg
     4861 gagcaggtac tgcgcgaacg tgaaggtatt cgcgctgcgg tgttccacga aggtatgtcg
     4921 attatcgaac gtgaccgcgc tgccgcctgg tttgccgaag aagacaccgg cgcacaggta
     4981 ctgctgtgct cagaaatcgg ttctgaagga cgtaacttcc agttcgccag ccacatggtg
     5041 atgtttgacc tgccattcaa cccggatcta ctggagcagc gtattggtcg tctggatcgt
     5101 atcggccagg cgcacgatat tcagatccat gtgccttatc tggagaaaac cgctcagtcg
     5161 gtgctggtgc gctggtatca cgaaggtctg gatgcatttg agcacacctg cccgaccgga
     5221 cgcactattt acgatagcgt atacaacgat ctgattaact atctggcttc accggttcaa
     5281 accgaaggct ttgacgatct gatcaaaaac tgccgcgagc aacatgaagc gctgaaagca
     5341 cagctggaac agggtcgtga ccgcctgctg gaaatccact ccaacggtgg cgaaaaagcc
     5401 caggcactgg cagaaagcat tgaagagcag gatgacgata ccaacctgat cgccttcgcc
     5461 atgaacctgt tcgatattat cggtatcaat caggacgatc gcggcgacaa catgatcgtg
     5521 ctgacgccgt ccgatcatat gctggtgccg gacttccctg gcctgtcgga agatggcatc
     5581 accatcacct ttgatcgtga agtggcgctg gcgcgtgaag atgcacagtt tattacctgg
     5641 gagcatccgc tgatccgcaa cggtctggat ctgatccttt ctggcgatac tggtagcagc
     5701 acgatttcac tattgaaaaa caaagcgttg ccggtaggta cgctgttggt ggaactgatt
     5761 tatgtcgttg aagcccaggc tccgaagcag ttgcagctca accgcttcct gccaccgacg
     5821 ccggtacgta tgctgctgga taaaaacggc aacaacctgg cggcgcaggt agagtttgaa
     5881 acctttaacc gtcagcttaa cgcggttaac cgtcacaccg gcagcaaact ggttaacgcc
     5941 gtgcagcagg atgttcacgc tatccttcag ctgggtgaag cgcagatcga gaaatctgcc
     6001 cgtgcattga ttgatgccgc acgtaacgaa gccgacgaaa aactgtctgc cgagctgtct
     6061 cgtctggaag cgctgcgcgc agtgaacccg aacattcgtg acgacgaact gaccgccatt
     6121 gagagcaacc gtcagcaggt aatggaaagc ctggatcagg caggctggcg tctggatgcc
     6181 ctgcgtttga tcgttgtaac gcatcagtaa cggagccgaa aatggggatg gaaaactaca
     6241 atccaccgca ggaaccctgg ttggttatcc tgtatcagga tgaccacatt atggtggtta
     6301 acaagccgag cggtttgttg tctgtgccgg ggcgtctgga agagcacaaa gacagcgtga
     6361 tgacgcgcat tcagcgtgat tatccgcagg cagaatcggt gcatcgtctg gatatggcta
     6421 ccagcggcgt gattgtagtg gcgctgacca aagccgcgga gcgggagtta aaacgccagt
     6481 tccgcgagcg cgagccgaaa aagcagtatg tagcccgcgt ctgggggcat ccatcccctg
     6541 cggaaggtct ggtggatctg ccgctgattt gcgactggcc aaaccgcccg aaacagaaag
     6601 tctgttacga aacgggtaaa cctgcgcaga cggaatatga agtggtggag tatgcggcgg
     6661 ataacacggc aagagtggtg ttaaaaccga ttaccgggcg ttcgcatcag ctgcgtgtgc
     6721 atatgctggc gctgggtcat ccgattctcg gcgatcgttt ttatgcatca ccagaagcga
     6781 gagcgatggc accgcgtttg ttgctacatg cagagatgtt gacgattacc catccggcgt
     6841 atggcaatag tatgacgttt aaagcgccag cggattttta atcgtcacgc tggcgcagtt
     6901 cggtaggcgg ataaggcgtt cacgccgcat ccggcaattg tgcgccgatt gcctgatgcg
     6961 acgcgggccg cgtcttatca ggcctacagt ttaaaactta tttaaaccct ttctgctgct
     7021 ttatcagctc atatgcctgc tgaatttcct gcgctttctg cttcgccatc tccatcatct
     7081 caggcggcaa acctttcgcc accagcttat cgggatggtg ttcactcatc agcttacggt
     7141 aggcacgttt gatggtggtc gcatcatccg tcggcttcac gcccagcaca ttacaggcat
     7201 cttccagcgt cgggccacgc tgcgcttgct gccagttacc accgccagat tgctgctgat
     7261 aaccgccgcc aaactgtgca ccgccctgca tcatgcgcaa aaactggtca aactgggcgc
     7321 gggagatccc taattcttca gcaatgacat acagcaccgc ccgttcattc gggtgcagtg
     7381 aaccatcagc aaacgccgcc tgaatctgga tctccagaaa catacgaatt aagtcaaaac
     7441 gaccaaagca gacactgcga aactggcgca tcttttcgcg cagcgggtaa ttatctgatt
     7501 ttcccacccg gaacgcgttt tgcgccgcag tacgggaagc gccatgaagg ttcattcggt
     7561 ccatcaactg gctggcgata tgaatatcag cctccgtgac gcgacctttg gatttggtta
     7621 aatgccccat cacttcaaaa gtggtggcaa aaaacagcgc ctgacgctca cgctggttgg
     7681 cgaaccacgc cattttacgg ctacgggctt tatcaaacat atggccaatt aacaggccta
     7741 acactacgcc ccaaaagccg ccgcccatca gtaaggccac ggccacgcca atgatttttc
     7801 cccaatactg catatattcc ccaaatcgac acacggatat cagggctatc tcccacaata
     7861 taaaggtgct tttaccgttt tccggctgcg gtcaattgtg ggacatcgcc tataatttgc
     7921 attatcatac ctgtcattca atgccgtgcc taacaccact gacgctattc ggacaggatt
     7981 aacactagcg tagagatgac gagtacgtta gtctctgacc gtttgtcacg cgcaacgtta
     8041 ccgatgatgg aacaataaaa tcaacgtatg aaaaaacgta tccccactct cctggccacc
     8101 atgattgcca ccgcccttta tagtcaacag ggactggcag ccgacctcgc ctcacagtgc
     8161 atgttgggcg tgccaagcta tgaccgtcct ctggtacagg gcgataccaa tgacttaccc
     8221 gtgactatca atgctgacca cgcgaaaggg gactacccgg atgacgccgt gtttactggc
     8281 agcgtggata tcatgcaggg taacagccgt ctgcaggccg acgaagtgca gctccatcaa
     8341 aaagaggcac caggacaacc ggagccggta cgtaccgttg atgcgctcgg taatgtccat
     8401 tacgacgata accaggtgat cctcaaaggg ccgaaaggct gggcgaatct gaacaccaaa
     8461 gataccaacg tctgggaagg tgattaccag atggtgggtc gccagggtcg cggtaaagcg
     8521 gacctgatga aacaacgtgg cgaaaaccgc tataccattc tggataacgg tagctttacc
     8581 tcctgtctgc cgggttctga cacctggagc gtggtaggta gcgaaattat tcatgaccgc
     8641 gaagaacaag ttgcggagat ctggaacgcc cgctttaagg tgggtccggt accgatcttt
     8701 tatagcccct atttgcagtt gccggtgggt gacaaacgtc gctctggttt cttgatcccg
     8761 aacgccaagt acaccaccac caactacttt gagttctacc tgccatatta ctggaacatc
     8821 gcgccaaata tggatgccac catcacgccg cattatatgc atcgtcgtgg caacatcatg
     8881 tgggagaacg aattccgcta cctctcccag gcgggcgctg gcttgatgga actggactat
     8941 ctgccttcag ataaagtcta tgaagatgaa cacccgaacg atgacagttc acgtcgttgg
     9001 ttgttctact ggcaacactc cggggtcatg gatcaagtgt ggcgtttcaa cgtcgactac
     9061 accaaggtca gcgatcctag ctacttcaat gatttcgata acaagtacgg ttccagtact
     9121 gacggctacg caacgcaaaa attcagcgtt ggctatgcgg tgcaaaactt caatgccacc
     9181 gtttcaacca agcagttcca ggtctttagc gagcagaaca ccagtagcta ctcggcagag
     9241 ccgcagttag acgttaacta ctaccagaat gatgttggtc cgtttgatac gcgtatttac
     9301 ggccaggcag tgcactttgt taacaccaga gacgacatgc ctgaagcaac ccgtgttcac
     9361 ctggaaccga ccatcaattt gccgctctct aataactggg gcagcatcaa taccgaagcg
     9421 aagttgctgg caacccatta tcagcaaacc aatcttgact ggtataactc cagaaacacg
     9481 accaagctgg acgaatccgt taaccgcgtt atgccgcaat tcaaagttga cggcaaaatg
     9541 gtctttgaac gcgatatgga aatgctggct ccgggttata cccaaacgct ggaaccgcgc
     9601 gcgcagtatt tgtacgtgcc gtatcgcgat cagagcgaca tctataacta cgactcgtct
     9661 ctactgcaat ctgactactc tggcctgttc cgggaccgga cttacggcgg tcttgaccgt
     9721 attgcctccg ctaaccaggt gacgaccggt gtcacatctc gcatatatga tgatgctgcc
     9781 gttgaacgtt ttaatatttc cgttggtcaa atctactatt tcacggagtc tcgcactggc
     9841 gatgacaaca taacatggga gaatgacgac aaaacgggct cactggtgtg ggcaggcgat
     9901 acttactggc gtatctccga gcgttgggga ttgcgtggcg ggattcagta cgatacacgt
     9961 ctggataacg tagcgaccag taactccagc attgaatacc gtcgggatga agaccgtctg
    10021 gtacagctga attaccgtta cgccagcccg gaatatattc aggctacgct gcctaagtac
    10081 tattccactg ctgagcaata taagaatggt atttcgcagg taggtgctgt cgccagctgg
    10141 ccaattgccg atcgttggtc cattgttggg gcctactact acgacaccaa tgcgaacaag
    10201 caagccgact ctatgttagg tgtgcaatac agctcctgct gctatgcaat tcgcgtcggt
    10261 tacgagcgga agctgaacgg ttgggataac gataaacaac atgcggtata tgacaacgca
    10321 atcggcttta acatcgaact tcgcggcctg agctccaact acggtctggg tacgcaagag
    10381 atgctgcgtt cgaacattct gccgtatcaa aacactttgt gatttgttga tttaccacgt
    10441 aatccgcagt gcggttaatt gaaatggaaa aagtatgaag aactggaaaa cgctgcttct
    10501 cggtatcgcc atgatcgcga ataccagttt cgctgccccc caggtagtcg ataaagtcgc
    10561 agccgtcgtc aataacggcg tcgtgctgga aagcgacgtt gatggattaa tgcagtcggt
    10621 aaaactgaac gctgcgcagg caaggcagca gcttcctgat gacgcgacgc tgcgccacca
    10681 aatcatggaa cgtttgatca tggatcaaat catcctgcag atggggcaga aaatgggagt
    10741 gaaaatctcc gatgagcagc tggatcaggc gattgctaac atcgcgaaac agaacaacat
    10801 gacgctggat cagatgcgca gccgtctggc ttacgatggt ctgaactaca acacctatcg
    10861 taaccagatc cgcaaagaga tgattatctc tgaagtgcgt aacaacgagg tgcgtcgtcg
    10921 catcaccatc ctgccgcagg aagtcgaatc cctggcgcag caggtgggta accaaaacga
    10981 cgccagcact gagctgaacc tgagccacat cctgatcccg ctgccggaaa acccgacttc
    11041 tgatcaggtg aacgaagcgg aaagccaggc gcgcgccatt gtcgatcagg cgcgtaacgg
    11101 cgctgatttc ggtaagctgg cgattgctca ttctgccgac cagcaggcgc tgaacggcgg
    11161 ccagatgggc tggggccgta ttcaggagtt gccgggtatc ttcgcccagg cattaagcac
    11221 cgcgaagaaa ggcgacattg ttggcccgat tcgttccggc gttggcttcc atattctgaa
    11281 agttaacgac ctgcgcggcg aaagcaaaaa tatctcggtg accgaagttc atgctcgcca
    11341 tattctgctg aaaccgtcgc cgatcatgac tgacgaacag gcccgtgtga aactggaaca
    11401 gattgctgcc gatatcaaga gtggtaaaac gacttttgct gccgcagcga aagagttctc
    11461 tcaggatcca ggctctgcta accagggcgg tgatctcggc tgggctacac cagatatttt
    11521 cgatccggcc ttccgtgacg ccctgacccg cctgaacaaa ggtcaaatga gtgcaccggt
    11581 tcactcttca ttcggctggc atttaatcga actgctggat acccgtaatg tcgataaaac
    11641 cgacgctgcg cagaaggatc gtgcataccg catgctgatg aaccgtaagt tctcggaaga
    11701 agcagcaagc tggatgcagg aacaacgcgc cagcgcctac gttaaaatcc tgagcaacta
    11761 atggttaaaa cccaacgtgt tgtgatcact cccggcgagc ccgccgggat tggcccggac
    11821 ttagttgtcc agcttgcaca gcgtgagtgg ccggtcgaac tggttgtttg tgccgatgcc
    11881 actctcctta ccgaccgggc agcgatgctc ggtttgccgc tcaccctccg cccttattcc
    11941 cccaactccc ctgcacaacc gcaaactgcg ggcacattaa cgctacttcc tgtcgcgcta
    12001 cgcgaatctg tcactgcggg gcagttagcg attgaaaatg ggcattacgt ggtggagacg
    12061 ctggcgcgag cgtgtgatgg ctgtctgaac ggtgaatttg ctgcgctgat cacaggcccc
    12121 gtgcataaag gcgtcattaa cgacgcaggt attccgttta ctggtcatac cgagtttttc
    12181 gaagagcgct cgcaggcgaa aaaagtggtg atgatgctgg cgaccgaaga acttcgcgtg
    12241 gcgctggcaa cgacgcattt accgctgcgc gatatcgcag atgctatcac ccctgcactt
    12301 ttgcacgaag tgattgctat tttgcatcac gatttgcgta caaaatttgg tattgccgaa
    12361 ccgcgcattc tggtctgcgg gctgaatccg cacgcgggcg aaggcggtca tatgggtacg
    12421 gaagagatag acaccattat tccggtgctc aacgagctgc gggcgcaggg gatgaaactc
    12481 aacgggccgc tgcctgccga taccctgttt cagccgaaat atctcgataa cgccgacgcc
    12541 gtgctggcga tgtaccacga tcagggtctt cccgtgctaa aataccaggg cttcgggcgc
    12601 ggtgtgaaca ttacgctggg cctgcccttt attcgcacat cagtggacca cggcaccgcg
    12661 cttgaactgg cgggacgtgg cgaagccgat gtcggcagtt ttattacggc gcttaatctc
    12721 gccatcaaaa tgattgttaa cacccaatga ataatcgagt ccaccagggc cacttagccc
    12781 gtaaacgctt cgggcaaaac tttctcaacg atcagttcgt gatcgacagc attgtttctg
    12841 ccattaaccc gcaaaagggc caggcaatgg tcgaaatcgg ccccggtctg gcggcattga
    12901 ccgagccggt cggcgaacgt ctggaccagc tgacggtcat cgaacttgac cgcgatctgg
    12961 cggcacgtct gcaaacgcat ccattcttag gcccgaaact gacgatttat cagcaggatg
    13021 cgatgacctt taactttggc gaactggccg agaaaatggg tcagccgctg cgtgttttcg
    13081 gcaacctgcc ttataacatc tccacgccgt tgatgttcca tctgtttagc tatactgatg
    13141 ccattgccga catgcacttt atgttgcaaa aagaggtggt gaatcgtctg gttgcaggac
    13201 cgaacagcaa agcgtatggt cgattaagcg tcatggcgca atactattgc aacgtgatcc
    13261 cagtgctgga agtaccgccg tcagccttta caccaccacc caaagtggat tccgccgtcg
    13321 tgcgcctggt tcctcatgca acgatgcctc acccggttaa agatgttcgc gtgttgagcc
    13381 gcatcaccac tgaagccttt aaccagcgtc gtaaaaccat tcgtaacagc ctcggcaacc
    13441 tgtttagcgt cgaggtgtta acgggaatgg ggatcgaccc ggcgatgcga gcggaaaata
    13501 tctctgtcgc gcaatattgc cagatggcga actatctggc ggagaacgcg cctttgcagg
    13561 agagttaacg atgatcaatt cgccccgagt gtgtattcag gttcaaagcg tctacattga
    13621 ggcacaatct tcacctgata atgaacgtta cgtttttgct tataccgtaa ccatacgcaa
    13681 tctggggcga gcgccagtgc agttgttggg gcgttactgg ctgatcacca atggcaatgg
    13741 ccgtgaaacc gaagtccagg gcgaaggagt ggttggcgtc cagccactta tcgcgcctgg
    13801 cgaagagtac cagtacacca gcggtgcaat cattgaaacc ccgctgggca ccatgcaggg
    13861 tcactacgaa atgatcgatg aaaatggcgt ccctttcagc atcgacattc ccgtattccg
    13921 actcgccgtt cccacactca ttcattaaaa gaatatggcg acatacctta ttggcgacgt
    13981 tcatggttgt tacgatgaac tgatcgcatt gctgcataaa gtagaattta cccctgggaa
    14041 agataccctc tggctgacgg gcgatctggt cgcgcgcggg ccgggttcgc tggatgttct
    14101 gcgctatgtg aaatccttag gcgacagcgt acgtctggtg ctgggcaatc acgatctgca
    14161 tctgctggcg gtatttgccg ggatcagccg caataaaccg aaagatcgcc tgacaccgct
    14221 gctggaagcg ccggatgccg acgagctgct taactggctg cgtcgccagc ctttgctgca
    14281 aatcgacgaa gagaaaaagt tggtgatggc ccacgcaggg atcacgccgc agtgggatct
    14341 acagaccgcc aaagagtgcg cgcgcgatgt agaagcggtg ctatcgagtg actcctatcc
    14401 cttctttctc gacgcaatgt acggcgatat gccaaataac tggtcaccgg aattgcgggg
    14461 gctgggaaga ctgcgtttta tcaccaacgc ttttacccgt atgcgttttt gcttcccgaa
    14521 cggtcaactg gatatgtaca gcaaagaatc gccggaagag gcccctgccc cactgaaacc
    14581 gtggtttgcg attcctggcc ctgtcgctga agaatacagc atcgcctttg gtcactgggc
    14641 atcgctggag ggcaaaggta cgccggaagg tatatacgcg ctggataccg gctgctgctg
    14701 gggtggtaca ttaacctgcc tgcgctggga agataaacag tattttgtcc agccgtcgaa
    14761 ccggcataag gatttgggcg aagcggcggc gtcttaaaca cagcctgata taggaaggcc
    14821 ggataagacg cgaccggcgt cgcatccggc actagccgta aattctatac aaaattaccg
    14881 ccgctccaga atctcaaagc aatagctgtg agagttctgc gcatcagcat cgtggaattc
    14941 gctgaatacc gattcccagt catccggctc gtaatccggg aaatgggtgt cgccttccac
    15001 ttctgcgtcg atatgcgtta gatacagttt ctgcgctttt ggcaggaact gctcataaac
    15061 gcgaccgccg ccaatcacca tgatttctgg tacgtcacca cacgccgcga tggcttcatc
    15121 caccgacttc acccacgtta cgcgatcgtc cgtacccggt tgactgctga ggataatatt
    15181 tttgcgtcct ggcaacggac gaccgattga ttcccaagta tggcggccca taatcacggg
    15241 tttatttaag gtgttgcgtt taaaccaggc gagatcggca ggcaggttcc acggcatggc
    15301 gttttccatg ccgataacgc gatctaccgc taacgccgca atcagactga tcattgagat
    15361 ttcccgataa aaaaaattgt cgccactata cgtaaagcgt aaaccgtcgt cgactggtgc
    15421 gaggatgatg ttgaggaaaa ttttatattc tgctggcgag tccacgctct ctccctggac
    15481 tcgccgcatt acaatgaaac aaaaacaaac agttagctgt aaagtgtgat ttacgtcact
    15541 ctttattagg atgagggttt cgtttccggt tcatccgcca tgttgccggt atgtttacct
    15601 tcttcggttc cctgccagcc atgtcgttga attaatgaca gatgttcgcg gtcctcggta
    15661 atgatctcac ttaacatcgc gctggtgcgt ttatagaccg ccgcgcgggc tttggtgtcg
    15721 ttctcaacca ttgccatctc ttccaccatc tgaatattaa agcggcggaa cacatcggca
    15781 cgttctcgcg cttcatacgg ccccagacct aaactttcca gtgccagacg cccggttttc
    15841 agcgcacctt cgaaggtttc acgctccggc ttttcaacgc ctgcctgacg caaacgaatg
    15901 tagtggtcga catcgcgggc gcgggcaata atctgcaaat gcgggaagtg ttctttcacc
    15961 atctctgtca gttgcaggtt ggtttgcgga tcgtcgatgg cgttaatcag cacttccgct
    16021 ttcgccgctc ccgcagattc cagtaaatcc atccgcgtgg catcgccata aaacactttc
    16081 ataccaaatt tacgcaaggt ttcgatatgg tccggatcgt gatcgagcac caccattttc
    16141 accccgctgg agagcagtaa acgtccggtg atctgcccaa aacgaccgaa tccggcaatg
    16201 attacccgcg gctgttcttc gtcgatctca tcagcttcac gcgcctcttc agtagaagat
    16261 tgctcaaggc gattgaggat caccagcaga atcggcgttg ccgccatcga cagcgccacc
    16321 gccagggtca gcgatttcgc ccactccggt tccagcacat tcgccatctg cgccgtgcca
    16381 aataccacaa aggcaaactc actgccctgc cctaacaaca ccgcaaacca acgacgctgt
    16441 ttatttggca cttgcaacgg tcgggcaatc agccacagca tggcgatttt gatgatgagg
    16501 aaaccgagca gcaaaatgac aatgcgcaat gggttttcaa gcagcgtgcc aaagtctatc
    16561 gacatgccaa caccgatgaa aaacagcccc aacagcaaac ctttaaatgg ttcgatatcg
    16621 ctctccagcg catgacggta ttccgagctt gccagcagta cgcccgccag aaacgcgccc
    16681 atcgccatcg acaagccgac ctcttccagc agcaaaccaa agccaaacac gaggaataac
    16741 gccacggcac taaacacttc ccgcaagcca gagcgggcta caaaacgcag cgccggacgc
    16801 gtgacatagc gccccagcaa taccaccagc accagcgcac ccgccacttt taacgccgag
    16861 agagcaaatg cgcccatcgt cgtcgaggca ctgctcgttg ccagtagcgg aatcatcgcc
    16921 accagcggga tcgccgcgat atcctggaac agcagcaccg caaaggcact gcgacccatt
    16981 tgcgtcacca tcagattgcg ttcattcatc gcctgcatgg caatcgccgt agaggagagc
    17041 gccagcgtca tgccgatcaa ttccgcgacc tgccagtgca gcccaagtaa catgcagaac
    17101 agccccagca ggccgccgca aatcaccatc tgcaatgcgc caccgccgaa cactgccgca
    17161 cgcagcttcc acagcctttg tggatcgagt tcgaggccga taataaacag catcagcacc
    17221 accccaatct cggcaaagtg cagaatagat tcggcatcgg tcaccagtcg cagcccccac
    17281 gggccaataa tgcagccggc gatcaggtag ccaagtaccg atcccagacc aagacgtacc
    17341 gcaatgggta caatcagcgc tgccgaaccg agataaatca gcgcctgaat cagcgtatgg
    17401 ctatccatga tgggcctcct gccattccag cagacgttgc ttatagtgac gcgcctgccc
    17461 ttcgagggtt tcgtcgtcac aaataaaggt gcagtgcatg gcaaacggtg gcagccagtt
    17521 cagcccgcag tagattgccg tcgcctgtag cggctgcgac agcacatcaa agcccggatg
    17581 cgcaccaatt tcaaaatggc tttccccgcc gccggtcgtc accgcccaca gcaaatattt
    17641 gccatgcagc gccttgccgc cgtgaccgta agcccagccg tgcgacaaaa ctttatcgat
    17701 ccaaagttta agaagcggag gaatgctgta ccactgcatc ggatgctgcc agacgatcag
    17761 atcggcgcga gacagcgcct cctgctcggc ggcaatatcg atattgaagt caggatagag
    17821 ttgataaaga gagcgaattt cgacgccttc cagcgtcctt gcctgttcaa gcatccgttt
    17881 attcgcatgg gaatgatgcg gatacggatg cgcataaatt ataagaatca taggtagccc
    17941 gcgtgattac gcctttgttt tcccacaaag tgtagtcata tattcacgcg gcttatagtc
    18001 aataatattg attcagaaaa gagagaaatc tgaacaattt accccattct ctgcccgcgg
    18061 atcgtcatat tgctggtttg cgccagtgac atccctcgag tctccggggc aaacgctacg
    18121 gaaatcaaca agccaaacag cgagataccc gcctccatta gcatcgtgtt actgataccg
    18181 taattattga taaagatcgg cagtgcccag gtcgaaacaa tggtgccaat acggcttaag
    18241 gacataatca cgcccacggc agaggcgcgg atatctgtcg ggaagagttc attaggatag
    18301 agccactgca aattacccgg cccgccagag aaaaaggcat acaccgcaaa ggccatcact
    18361 accagccaga tccccatatc cgggatcagc cccaaaaccg ccagcgccag cgtcatcatg
    18421 gcaaagctgc caatcaacaa tggacgccgt ccggcagtgt ttaaccacag catcggcgga
    18481 atacagccga gcataaagaa caggctaatc accacgttcc ccagcgccgc gtttttgcca
    18541 acacccaatc ccaacaaacc aacgatttgc gggccaaagg tgtaaatggc gaacattggg
    18601 atcacctggc aggtccagat ggcggcaaca aacagaacaa aaggaaaatg gcggcgatta
    18661 aacagatcgc gaaaacgggt ttgctacggc tgctcttcat cgaaagccac cggttcgcca
    18721 aacagtttta tcatcatctc ttcgcactct tttactcgcc ctttgcgtaa taaccagcgg
    18781 ggagattcag gcagttcgaa tcgaccaatc aaaatcaaca aacaggggat cgccgcgcta
    18841 cccagcatcc agcgccagcc gccttccaca tcataaagcc agtagccgac cagatcggcg
    18901 caggtcgcgc cgacatacca catggcggcg ataaagctga tggaaaatgc ccgctgacgg
    18961 gtactggaga actcggtgat cattgaggtg gcgatgggat aatctgcacc gatgacaatg
    19021 ccgataagta cccgcatcac caacagttcg acgggggatg aaacaaacat cgtcgccacc
    19081 gatatcacgc cgatggcgat gatatcaatg aggaacattt tgcgccgtcc tactttatcg
    19141 gaaatatagc caaacagcga tgtgccaacg aacagcccgg cgagcgttcc cgcgcccaac
    19201 aagccaatcc agtcagcgtc cagttttagc gccggagtca gttgctccag cgccacgcca
    19261 atcattacca gtacataacc atccagaaac ggaccaccgc ttccccacag caaaatgcgg
    19321 cggtgaattg aggagaattt gagatcgtca aagtttctgg acggttgcat gggttattcc
    19381 tgtttttttg caatgagagt cagtacggcg ttacgtatca aaccaacatc agccgtaacg
    19441 gaactccaca ccaaaggtgc cgcgcgggta ttcccactgt tccagcgccg accccagccc
    19501 cagaatgcga caggtgccgc actccagaca tccggcgtaa tcgaagcgca cactgccgtc
    19561 atcctgcttc ttgtacagac ctgcggggca cgctttcacc agcagctcca gggcctgttt
    19621 atcggcatcg gccttcacaa caatgtgcgg atgctcttca tcgacattga atttattgac
    19681 gcccagtttg acgtccacat tgacgggaga agtcataaaa cggtcactcc tttcatgcca
    19741 tccttgatca gattgatgaa gcccactttc ttgccgtggc ggaggatttt cttgcgcatc
    19801 agttccggcg cactgccgtc aatggtgaac aggtcacgcg ccacgcccac cgccagttcc
    19861 gggtagccgc taaacatgcg tgggttatca aggaaggccg gtagtttctg gtacatacgc
    19921 atatcgcgca gcgggccact ctcaagatgc tgacgatatt ccgccagttt ttgcttactg
    19981 aaatcgtcgc ttttcatcgc tgaaagcacg gtttttgctg cggcttcccc ggcggcaatc
    20041 gccagatcca taccgcgaat ggtaaaaccg aggttcatac acattccggc ggcatcaccg
    20101 gcaatcaata cgccgtcacc aaccaactcc ggcagcatgt tgatgcctgc ttccggcact
    20161 acgtgagcgg aatattccac cagcttgccg cccgcgatca gcggtgccac tgccggatgc
    20221 tgtttgaaat cttccagcat ttgcggcacc gatttttttg cgtcatgcag atgatgcaga
    20281 ccacaaacca gccccagcga cagggtattt tcattggtat aaaggaagcc gccgcccatc
    20341 aggccatcgg tgggtgatcc cgcaaacaga caagccgcgc cctgattacc ctgcaactga
    20401 aaacggtctt cgattaccga cttcggtaac tcgatcagtt ccttcacgcc aaccgccaca
    20461 tccgtcggtt taacgcgttt tgccatcccc agcttttcgg caaggatgga gttcactcca
    20521 tcagcaagga tcaccgtttt cgcttcaatc acatcgccat cggcttctac accgacgact
    20581 ttgccatcgc gctgtacgag gttatcgacg cggatcccgg taattaactg cgcgcccgct
    20641 tcttcggcct gctccatcag ccaggcatca aatttactgc gcaaaacgga gtaagaacgc
    20701 tgggatggcg aggtttcgtc accgttgcag tagtccatag tcatcgctga cttttccgtc
    20761 ataaacgcga gtttttcatg ggtgatcagg cgttctaccg gggcggattc ggcgaaacca
    20821 ggaataatgt gttccaggct gtgggcatag agacgcccgc cggtgacgtt cttggcacct
    20881 gcggaattgc cacgctcgat aactaacact tgcgcccctt cgcgggcgag caccagtgcg
    20941 gcaaccgaac cggcaagccc agcaccgacg atgatggcgt caaagatatc ttcggacata
    21001 agagctccct gtcagcggca gggccaaaat gcagccctgc cagagtggat caacgcgcta
    21061 aagctgcggt cagcgccgga aggatcttca cggcgtcacc aacaatgccg taatccgcgt
    21121 actggaagat cggcgcattt ttatctttat tgatggcgaa aatggtttgc gatgcattag
    21181 caccaaccat gtgctggatc tgcccggaga tccccaccgc caggtacagt tcaggtttca
    21241 gcatcaggtt ggagataccg acatagcgtt cgtgctccat ccatttttcg ttttccgcca
    21301 ccggacgaga acaggccaac tccgcaccta tcgccttgca aagctgttct gccagcgcaa
    21361 tgttctcttt gctgccaata ccgcgaccga cgctgaccac cagacgggct ttgtcgagat
    21421 cgacgctgtt gctctggcgc gcctgggttg ccgtgcgggt aatcgccaca gccggagcct
    21481 gccactccac ggtgtgcgtt tcgccagtgc gtgacgcgtc tggctgagcc gcatcgaacg
    21541 tgccgctgct gatggtcagt accgcatacg gcgtggcaat gcgttcttcg ccaatcgcca
    21601 gaccaccgta aaccatatgt ttcactgttg ctttaccgtc ctgtacgctg acggtgctgg
    21661 catcgttaga caccgccgct ttaaggcgat aacccagttt tgccgccagt aatttgccgc
    21721 gacgggtgtt tggcagcagc accaggccgt ctgcgccgtg ctggcgaata gtgtcagcca
    21781 tgacaccggc gtaatcttcg atcatccgat cgtccggttt gccgtttaat ttccagacat
    21841 gattagcgcc gagctggatt gcctgtgcgc cgtcggcatc attgaggaca aaggtgttga
    21901 tttgattagc taaagcctgc gcaccgttca tcagttccgg cagacgagaa ggggtatcgc
    21961 tgaataccca gacttgagaa aacgtgttca tagcatcccc tgtaattaaa tgactttgcg
    22021 aagattttca gcaaatgcgg cgatctgttc ttcgccgtcg ccttcaatca cgatgcgctg
    22081 acgttcgcgc tgtttcggcg cggcaacctg ttgttctgac caggctgcct ctgcgttaaa
    22141 accaatatcc gccgccgacc atacctggac gggctttttc gccgcgccga gaatggcttt
    22201 catcgaagga atttgtgggg aattgatatc agtggaaaca gcaacaaccg caggcagcgg
    22261 aatgcttaag gtttcggttt catcttccag ttcgcgctca acggtgaggg tatctgccgt
    22321 cagggagata attttgctga cgccgttaac tgccggaata ttgaggattt cgcccaccag
    22381 cagaccaacc tgctgggcat aaaggtcgga agaaccatcg ccacagagga tcaaatcaaa
    22441 gcctgctttc tgggcggctg cagccagtgc gctcgccgtt tgttgcggca gtgcctgctc
    22501 gaactggtca tcaatcacca caatcagttc atccgggccg cgcgatagta catctttacg
    22561 ccctttggcg ttggtcaggg ctttaccgcc cacacttaag gccgtcacct gcgcctctgc
    22621 tgcctgttgc tttagctggc aagccgcttc aatagcgttc agatcgtatt ggcttatttt
    22681 ggcatcggct ttgctgaagt ctaatgaacc atcagcatta ttgaccgcaa tatcctgttc
    22741 atcaggcacg cacttatagc aagtaataat cttcattgca tctccagaaa tcatgaagga
    22801 aacgtcgttc atatcggtgg tgttaattac agatatcacg ccctttaatt tggaaataaa
    22861 actttaatca ccaatattga aaatgtcaca cgcagataaa tccaactttc aatattgtta
    22921 agttcctcac caatattgaa aacatggcgt agaaaaaaga aattttcaat attgctttat
    22981 ggatcaccaa tattgaaagt cagatgctat ttttttactc acaacacaac ataacaaact
    23041 gattaataat caaaattaac gagaaaagcg ccctctattc acccgaaaaa aaatgtgata
    23101 acaatcacag aatgcagctt attgaatacc cattatgagt tagccattaa cgcgtccacg
    23161 aggttaataa taattatatc aaatgttaac aaaaataaaa caaacgggaa cgcaaaataa
    23221 atattcgttt tcacagtgga attaactcat gaagaatgaa aagagaaaaa cgggaataga
    23281 accgaaggtt ttctttccgc cgttaataat cgtcggcata ctttgttggc ttacagtcag
    23341 agatctggat gcagcgaatg tcgttattaa tgctgtattc agttacgtca ccaatgtatg
    23401 gggatgggca tttgaatggt atatggtggt gatgcttttc ggttggttct ggctggtgtt
    23461 tggcccgtat gccaaaaagc gtttaggtaa cgaaccacct gaatttagta ccgccagttg
    23521 gatctttatg atgttcgcct cctgtacgtc tgctgccgta ctgttctggg gatcgattga
    23581 gatctactac tacatctcca ccccgccgtt tggcttagaa ccgaactcga caggggcgaa
    23641 agagttgggg ctggcttaca gcttgttcca ctggggacct ctgccgtggg ccacttacag
    23701 cttcctttca gtcgccttcg cttacttctt ctttgtccgc aaaatggaag tgattcgccc
    23761 cagctcgaca ctggtgccgc tggtaggtga aaaacacgcc aaagggttgt tcggcactat
    23821 cgtcgacaac ttctatctcg tcgccttgat cttcgcgatg ggtaccagtc tgggccttgc
    23881 cacgccgctg gtgaccgagt gtatgcaatg gttgtttggc attccgcata ccctgcaact
    23941 ggacgctatc atcattacct gctggattat cctcaacgcc atttgcgtcg cttgcggtct
    24001 gcaaaaaggg gtacgtatcg ccagtgacgt gcgtagttac ctgagcttcc tgatgctggg
    24061 ttgggtgttc attgtcagcg gtgccagctt catcatgaac tacttcaccg attcggtggg
    24121 gatgttgctg atgtatctgc cgcgcatgtt gttctatacc gatcccatcg ctaaaggcgg
    24181 cttcccgcag ggctggaccg tgttctactg ggcatggtgg gtgatttatg ctatccagat
    24241 gagtatcttc ctcgcccgca tctcccgtgg tcgtactgtg cgtgaactgt gcttcggcat
    24301 ggtgctgggg ctgacagcgt caacctggat cctgtggact gtactcggta gtaacactct
    24361 gctgttgata gataaaaaca tcatcaacat tccaaatctg atcgaacagt acggtgtggc
    24421 gcgcgccatc attgaaacct gggccgctct gccactcagc accgccacca tgtggggctt
    24481 cttcatcctc tgctttattg ccaccgttac gctggttaac gcctgctctt ataccctggc
    24541 gatgtccact tgccgcgaag tacgcgatgg tgaagaacca cctctgctgg tgcgtatcgg
    24601 ttggtcaatt ctggttggca ttatcggtat tgttctgctg gcgctcggcg gcctgaaacc
    24661 gattcaaacc gccattatcg ccggaggatg cccgctgttc ttcgtcaaca ttatggtgac
    24721 gctctccttt attaaagacg cgaaacagaa ctggaaagat taattaaccc ccaaaatatc
    24781 aagaggttga aagatggatt ttaatttaaa tgatgagcag gaactgtttg tcgccggtat
    24841 ccgcgaactg atggccagcg aaaactggga ggcctatttt gccgagtgcg accgtgacag
    24901 cgtctacccg gaacgttttg tcaaagcact ggcggatatg ggtatcgaca gtctgctgat
    24961 ccctgaagag cacggtggtc tggacgcggg gtttgttact ctcgccgccg tgtggatgga
    25021 gctgggacgt ctgggggcac caacctatgt gctgtaccag ttgccgggcg ggttcaacac
    25081 cttcctgcgc gaaggcacac aagagcagat cgacaaaatt atggctttcc gcggcaccgg
    25141 taagcagatg tggaactcag cgattaccga accgggcgcg ggctccgacg tgggtagcct
    25201 gaaaacgact tatacccgta gaaatggtaa gatttatctt aatggtagta agtgttttat
    25261 taccagcagc gcctacaccc cgtacatcgt ggtgatggcg cgcgacgggg cttctccgga
    25321 caaacctgtc tacaccgaat ggtttgttga tatgagcaaa ccgggcatca aagtgaccaa
    25381 acttgaaaag ctcggtctgc gtatggatag ctgctgtgaa atcacctttg acgacgtgga
    25441 actggacgag aaagacatgt tcggtcggga aggtaacggc tttaaccgcg tcaaagaaga
    25501 gttcgaccat gaacgtttcc tggtagccct caccaactac ggtacggcga tgtgcgcttt
    25561 tgaagatgcg gcgcgctacg ccaatcagcg cgtgcagttt ggcgaggcta ttggtcgttt
    25621 ccagttgatt caggaaaaat tcgcccacat ggcgatcaaa ttaaactcca tgaaaaacat
    25681 gctgtatgaa gcagcgtgga aagcagacaa cggcaccatc acctctggcg atgcagcgat
    25741 gtgcaaatac ttctgcgcca atgcggcatt tgaagttgtg gatagcgcaa tgcaggtgct
    25801 gggcggtgtc gggattgcgg gcaaccaccg catcagccgc ttctggcgtg atctgcgtgt
    25861 agaccgcgtc tccgggggat ctgacgaaat gcagatcctg acgctgggtc gcgcggtgct
    25921 gaagcaatac cgctaagtcc gtgcgctgcc tgatgcgacg ctaatgcgtc ttatcaggcc
    25981 tacgggagca cgaatgtagg ccggataaag cgtttacgcc gcatccggca gtcatgcgct
    26041 acataaattt ttcaggagct aataatggat catctaccca tgccgaaatt cgggccgttg
    26101 gccggattgc gcgttgtctt ctccggtatc gaaatcgccg ggccgttcgc cgggcaaatg
    26161 ttcgcagaat ggggtgcgga agttatctgg atcgagaacg tcgcctaggc cgacaccatt
    26221 cgcgttcaac cgaactaccc gcagctctcc cgccgcaatt tgcacgcgct gtcgttaaat
    26281 attttcaaag atgaaggccg cgaagcgttt ctgaaattaa tggaaaccac cgatatcttc
    26341 atcgaagcca gcaaaggtcc ggcctttgcc cgtcgtggca ttaccgatga agtactgtgg
    26401 cagcacaacc cgaaactggt tatcgctcac ctgtccggtt ttggtcagta cggcaccgag
    26461 gagtacacca atcttccggc ctataacacc atcgcccagg ccttcagtgg ttacctgatt
    26521 cagaacggtg atgttgacca gccaatgcct gccttcccgt ataccgccga ttacttttct
    26581 ggcctgaccg ccaccacggc ggcgctggca gcactgcata aagtgcgtga aaccggtaaa
    26641 ggcgaaagta tcgacatcgc catgtatgaa gtgatgctgc gtatgggcca gtacttcatg
    26701 atggattact tcaacggcgg cgaaatgtgc ccgcgcatga gcaaaggtaa agatccctac
    26761 tacgccggtt gcggtctgta taaatgcgcc gacggctaca tcgtgatgga actggtgggc
    26821 attacccaaa ttgaagagtg ctttaaagat attggcctcg cacatctact cggtacgccg
    26881 gaaatcccgg aaggcactca gcttatccac cgtatcgaat gcccttacgg cccactggta
    26941 gaagaaaaac tcgatgcctg gctggcggca cataccatcg cagaagttaa agaacgcttt
    27001 gccgaactga atatcgcctg tgccaaagta ctgaccgtac cggaactgga aagcaatcca
    27061 cagtatgttg cccgtgaatc aatcactcag tggcaaacga tggatggtcg cacctgcaaa
    27121 gggccgaaca tcatgccgaa attcaaaaat aaccccggac aaatctggcg cggaatgccc
    27181 tcacatggca tggacacggc tgccattttg aaaaatatcg gctacagcga aaacgacatt
    27241 caggagttgg tcagcaaagg tctggccaaa gttgaggact aaacgcgtgg cgtcagtcgg
    27301 cttgcctgac tagcgcccgc ccaataacaa acgaacaaaa atggatagag gtgcaatgga
    27361 tatcattggc ggacaacatc tacgtcaaat gtgggacgat ctggcggacg tttacggtca
    27421 taaaacggcg ctgatttgtg aatccagcgg cggagtcgtt aaccggtata gttatcttga
    27481 gttaaatcag gagattaacc gcacgacaaa cctgttttat acgctgggga ttcgcaaagg
    27541 cgacaaggtt gctctacatc tcgacaactg cccggaattt atcttttgct ggttcgggct
    27601 ggcaaaaatt ggcgcgatta tggtgccgat taacgcccgc ctgttgcgcg aggaaagcgc
    27661 gtggatcctg caaaatagcc aggcgtgcct gctggtgacc agtgcgcaat tctatcctat
    27721 gtatcaacag attcagcagg aagatgccac tcaattgcgg cacatttgcc tgacagatgt
    27781 ggcacttccc gctgatgatg gcgtgagttc gtttactcaa ctgaaaaatc aacaacctgc
    27841 caccttgtgc tatgcaccgc cgctatcgac tgacgatacg gcggaaattc tcttcacctc
    27901 cggcaccacc tcccgaccga aaggtgtggt gattacccat tacaacctgc gcttcgctgg
    27961 atattactcc gcctggcagt gtgcactgcg tgacgatgac gtctacctga cggtaatgcc
    28021 tgcgtttcat atcgattgcc agtgtactgc ggcgatggcg gcgttttctg ccggggccac
    28081 ctttgtgctg gtcgagaaat acagcgcccg cgccttctgg ggacaggtac agaagtaccg
    28141 cgccaccgtt accgaatgta ttccgatgat gatccgtacc ttgatggcgc agccgccttc
    28201 agcgaacgat cggcaacacc gcctgcggga agtgatgttt tatctcaact tgtcggagca
    28261 ggaaaaagat gcgttttgtg aacgcttcgg cgttcgcttg ctgacgtctt atgggatgac
    28321 ggaaaccatt gtgggcatta tcggcgatcg ccctggcgat aaacgacgct ggccgtcgat
    28381 tggtcgggcg gggttttgct acgaagcgga gatccgcgac gatcacaatc gcccgctccc
    28441 ggcaggtgag atcggtgaaa tctgtattaa aggcgtacca gggaaaacca tcttcaaaga
    28501 gtattttctc aacccgaaag ccactgcgaa agtgctggaa gccgacggct ggctacatac
    28561 cggcgatacc ggatactgcg acgaagaggg ctttttttat ttcgtcgatc gccgctgcaa
    28621 tatgatcaaa cgcggtggcg agaatgtctc ctgcgtggag ctggaaaata tcatcgccac
    28681 ccatccaaaa attcaggata tcgtggttgt gggtattaaa gattcgattc gcgatgaagc
    28741 catcaaagca tttgtggtgc tgaatgaagg tgaaacattg agcgaagagg aatttttccg
    28801 cttctgcgaa caaaatatgg cgaaatttaa agtgccctct tatctggaga tcagaaaaga
    28861 tctgccacgt aattgctcgg ggaaaataat tagaaagaat ctgaaataaa tgaaacagca
    28921 gggaactacc ctgccaacta ataatcacgc tataaaacaa tatgcatttt ttgcagggat
    28981 gttgtcgtcc ctgaaaaagc aaaaatggag aaaaggaatg agtgaatcat tacatctgac
    29041 ccgcaatgga tcaattctgg aaattaccct tgatcgtcca aaagcgaatg ctattgatgc
    29101 aaaaaccagc tttgaaatgg gcgaagtatt tctaaatttc cgtgacgatc cgcaattacg
    29161 tgtcgccatt attaccggtg ccggagagaa gttcttttcc gcgggctggg atttaaaagc
    29221 ggcagcagaa ggcgaagcac cggatgctga ctttggcccg ggtggatttg cgggattaac
    29281 cgaaattttc aatctcgaca aaccggttat cgcagctgtg aacggctatg cctttggcgg
    29341 cggtttcgaa ctggcgctgg cggcagattt tattgtttgt gccgataacg ccagcttcgc
    29401 cctgccggaa gccaagctgg gcatcgttcc tgacagcggc ggcgtgctgc gtctaccgaa
    29461 gatcctgccg cctgccatcg tcaatgaaat ggtgatgacc ggcagacgaa tgggcgcaga
    29521 agaggcgctg cgttggggga tagtcaaccg cgtggttagc caggcggaac tgatggataa
    29581 cgcccgcgaa ctggcgcagc agctggttaa cagcgccccg ctggcgattg cggcgctgaa
    29641 agagatcttc cgcaccacca gcgaaatgcc ggtagaagaa gcgtatcgct atattcgcag
    29701 cggcgtgttg aaacactatc cgtcggttct gcattcggag gatgccattg aagggccgct
    29761 ggcgtttgcc gagaagcgcg atccggtgtg gaaaggacgt taacaaccgt gagctattac
    29821 gcctttgagg ggttaattcc ggtggttcac ccgacggcgt ttgtccatcc cagtgccgtc
    29881 ttgattggcg atgtgattgt gggagccggt gtctacatcg gcccactcgc ctcactgcgt
    29941 ggtgactacg ggcggttgat cgtgcaagcg ggagccaata ttcaggatgg ctgcattatg
    30001 catggctact gcgacactga caccatcgtt ggggaaaacg gccatatcgg gcacggagcg
    30061 atcctgcatg gttgtgtgat tggtcgcgat gcattggtcg ggatgaacag cgtgattatg
    30121 gatggcgcgg tcattggcga agagagcatt gttgccgcca tgagctttgt caaagcaggc
    30181 tttcacggcg agaaacgcca gttgttgatg ggtacgccag cccgcgctgt acgcagtgtt
    30241 agtgacgacg agttacactg gaaacggttg aataccaaag agtatcagga tcttgtcggg
    30301 cgctgtcatg catcgttaca tgaaacacag ccactgaggc aaatggagga aaatcgcccc
    30361 cgtttgcagg ggacgacgga tgtgacgcct aaacggtgat cttgccggat gcggcgtaaa
    30421 cgccttatcc aactctgctg gagagcaacc gtaggccgga taagatgcgc cagcatcgca
    30481 tccggcgata gctgtcggat gcggcataaa cgccttatcc aacccggctg gagagcaacc
    30541 gtaggctgga taagatgcgc cagcattgca tccagcaacc aggctgcgtg gtatcaacga
    30601 aatgacatta acgacgcata ctctttgaca acatcatctg ccacttctgc tcgcggttaa
    30661 gttccgtgag cggaacggca ggaatacgcg gcgtctttct tacgccgact aatttatccc
    30721 ggctgttatt gcgcaggcgc gcgtaaattt gctcatcgat atcgaccact tttaccagtc
    30781 gctggcactg gcatccccgt ccttccagct tattagggat catcttaact gcgcagctta
    30841 tttctgcgac ttccgacaga atataagtca gggtattaac cgccttgctg tgttcaatat
    30901 cgaaatgaat agagatctct cttgctatca cccaccgatt ttcagccatc atccattcgg
    30961 cgattaacaa gtagagtggt ttttcaacat atccttcaca catacgaact ccagtttcac
    31021 aaattaaaag tctggctatg actcgttttg aagcaattaa acaaggccat attaaaattg
    31081 tggatatatc gatagtatgc aacttcaccg tcgataag
//
LOCUS       DAESHI010000042        30851 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.042, whole genome shotgun sequence.
ACCESSION   DAESHI010000042 DAESHI010000000
VERSION     DAESHI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30851
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.042"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            34..372
                     /locus_tag="KW011_001974"
     CDS             34..372
                     /locus_tag="KW011_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001698482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HBH7660307.1"
                     /translation="MLKQRRNFCTGTERHANRFTTSASRSNIRYSLSDTHATPDGYPV
                     KQIGEHAWLIEKAGIVIHKCPRNPFTGNRIFALSCGDNQFGQDFTLYEALRTVDRLLR
                     GQSFIKQADL"
     gene            383..670
                     /locus_tag="KW011_001975"
     CDS             383..670
                     /locus_tag="KW011_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660308.1"
                     /translation="MTKDNAQGVFIRFIDFRGELLLRASAIDGVTPAGKNGADEATYV
                     YLNGTRLLVELPYQTVREIISEDEKARQVNGDEPYIEIICMDSEAEIQKAD"
     gene            682..924
                     /locus_tag="KW011_001976"
     CDS             682..924
                     /locus_tag="KW011_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HBH7660309.1"
                     /translation="MGKEYKTLINKALERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            921..1034
                     /locus_tag="KW011_001977"
     CDS             921..1034
                     /locus_tag="KW011_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660310.1"
                     /translation="MSIFISWLVLIISVVCAIGIMRIINSVKKIERFFTEE"
     gene            1121..1324
                     /locus_tag="KW011_001978"
     CDS             1121..1324
                     /locus_tag="KW011_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660311.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVVQVNLIRQVQESRR"
     gene            1321..1566
                     /locus_tag="KW011_001979"
     CDS             1321..1566
                     /locus_tag="KW011_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBH7660312.1"
                     /translation="MTILDYIAANPGCSSGEIAAALNTPTTTINAELRRLWRSGLVTR
                     KERKTGGRFSYQVNLMPFGCSNPLTQMFNQLLREIRA"
     gene            1708..2073
                     /locus_tag="KW011_001980"
     CDS             1708..2073
                     /locus_tag="KW011_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660313.1"
                     /translation="MILANDFLEYLLNTERDLAARVRDRYDMYLKSLPVPQLADGKIV
                     IDGRYMIDSHEGNYRLYRIEGGTPSVIGIYQRPSSAIVDVIADSIRITHRHADTEDTV
                     LEIQRLATVCRDTLNGMTK"
     gene            2080..4902
                     /locus_tag="KW011_001981"
     CDS             2080..4902
                     /locus_tag="KW011_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HBH7660314.1"
                     /translation="MTAEYIRDWQQPRHAVGREGTGIPAPESALSSWLDAHRAENERR
                     QEMADAAFSATPLGNLINKSLDAQEKQDKTITLAGDARKQARGAVDEAMASLRLLPSY
                     LRDPLIRHLSFLRKKQEADRRKGKKSWQAERYARGTLRKIFERLDRTDGHWLTPGYRS
                     LAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTMDVTLKA
                     YQMLARMALHLHAMPPHYDALTTDKDRRHEPDTELLPGAILRLTCAEWWKRKLWLLRC
                     EWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFTIDLETV
                     YYAGVSNPVHRKAEMMATMKGLELLAEARGDKAVFLTVTCPSKYHATTENGHPNPKWN
                     GATMRDSSDYLVNTFFATVRKKLNRDGLRWYGIRTAEPHHDGTVHWHMMVFAHPEEID
                     TIVSHTRDIAIQEDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGKNLDSRAVDGI
                     DPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRRLASQMA
                     RNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLADELNDY
                     GEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEAEDSARENGFDLQGGPAAPWTR
                     GNNCPRVQETDNNGTEQPEERPAPWPQLPDGVDVDEWMRSLKRHERRALMRSLRDKQA
                     KNSSDEMQSWTQSRKQQRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLRGGSLYV
                     DGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLTQLGASD
                     PEAAARLASTLQQDQNTMKTPVTVLSDMLRAIRDAEHAQRISETTERARRKADLLRGG
                     LTSGNKKQTETGLTNPVNEQKTRSDI"
     gene            4979..5938
                     /locus_tag="KW011_001982"
     CDS             4979..5938
                     /locus_tag="KW011_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HBH7660315.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGMQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNVIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAGFSGYGRVM
                     VVGGGAEIVAPAIREACGVNATFIADGVPQFALVNGLYAMDKE"
     gene            5943..6254
                     /locus_tag="KW011_001983"
     CDS             5943..6254
                     /locus_tag="KW011_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HBH7660316.1"
                     /translation="MTTPTRRISFYLKPAAVKNESEACAWLGSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWADDEALSVTQTRTERPVPQPVPTAQITSQIAGNIRALFP
                     E"
     gene            6315..6839
                     /locus_tag="KW011_001984"
     CDS             6315..6839
                     /locus_tag="KW011_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660317.1"
                     /translation="MKKYEYMLMKEALRAGLAPCPVSVEAVVGNTIFRTLRNRVSVKM
                     VHQPPKSTSMIYLPLPEAIKLMRSLKFAIWMQTQINKAVQNTFVKRLIPELAARCAKC
                     TRPALQFDLSLIQQATEAELYRLSPIRDAHTEASEMFRLMFQFNFAVRRTRVQVNRMM
                     QEQEELTQYKGGSQ"
     gene            6836..7075
                     /locus_tag="KW011_001985"
     CDS             6836..7075
                     /locus_tag="KW011_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660318.1"
                     /translation="MSNSARLQLGFSPLSKTIVLAKMRDLGDGTKRRVGNDRGRDVTN
                     EAAQLVWHLVMAEGGEINWELDDGSRMILRAEKSK"
     gene            complement(7767..8813)
                     /locus_tag="KW011_001986"
     CDS             complement(7767..8813)
                     /locus_tag="KW011_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBH7660319.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNPYALLDTQPTS"
     gene            complement(8813..10564)
                     /locus_tag="KW011_001987"
     CDS             complement(8813..10564)
                     /locus_tag="KW011_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HBH7660320.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDATATGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAEMHFLGTSAATAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTCFS
                     TPSTETHEAYAYWNGDRWNEKKAAHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFRNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTYRYNVEHIGIDATGGHGDAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            10719..11555
                     /locus_tag="KW011_001988"
     CDS             10719..11555
                     /locus_tag="KW011_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HBH7660321.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSIQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVESVATS
                     QADIIDRFNALETRHQQDSQKITLLTTELTALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            11579..12631
                     /locus_tag="KW011_001989"
     CDS             11579..12631
                     /locus_tag="KW011_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HBH7660322.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPAVQQR
                     FENASKDSTELTKKINVIGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLK
                     NRKYQCEQVNYDTFISYPQLDAWSAHSDFQSRISAQIARQVALDRIMIGFNGTSHADE
                     SNFSTNKLLQDVNVGWLEHIRTNASERVMNDVTLTSRNMDNTVAHAGKYANPDALVQD
                     ARSSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIG
                     GLGVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVE
                     DYGKCAMVTGLKFADS"
     gene            12677..13477
                     /locus_tag="KW011_001990"
     CDS             12677..13477
                     /locus_tag="KW011_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBH7660323.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKADIKKALLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYAVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSVFIEPLDTLWEI
                     VANQDMPDEVRAKLCKACAFARRGLSDADSMASSLKLLREAMHLNQNAGVKREIATLS
                     RALKKADSAAAPEDASAQQAQDESSESKKTTRKPATRKTTATQKAKRG"
     gene            13579..14073
                     /locus_tag="KW011_001991"
     CDS             13579..14073
                     /locus_tag="KW011_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HBH7660324.1"
                     /translation="MSMVARTEPGPAEDDITDTEDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAVAHVTRELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILCRPRIF
                     AELC"
     gene            14073..14270
                     /locus_tag="KW011_001992"
     CDS             14073..14270
                     /locus_tag="KW011_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HBH7660325.1"
                     /translation="MKVKTLEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCRQVFLEA
                     GQEVEMPEPEKKKQREMIQLW"
     gene            14264..14566
                     /locus_tag="KW011_001993"
     CDS             14264..14566
                     /locus_tag="KW011_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH7660326.1"
                     /translation="MVTGFFQRVSDWITFWASVTATTIGVMTISEKIALAGLLLGSLS
                     ALHAWRHRRLMETAMQRRNELIASILAQSDARPLHDSERQALNILRDNDTHDKAAR"
     gene            14547..15095
                     /locus_tag="KW011_001994"
     CDS             14547..15095
                     /locus_tag="KW011_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBH7660327.1"
                     /translation="MIKLPVKRYAVAAIVALGFSIAPHKLRTSPEAQIRIATWEDCRA
                     TPYRDIAGVMTVGCGSTGHVENRLYTETEVAGRWVNDMQHAENCINQNFSGNAMPQSA
                     FEAMTDAAFNLGCRNLMWFRDKNRNYHRTTIWKHAQAHNWPDMCNRLTDFVNSGGERS
                     QGLVNRRTDFRAWCLRDTGGQS"
     gene            15092..15565
                     /locus_tag="KW011_001995"
     CDS             15092..15565
                     /locus_tag="KW011_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660328.1"
                     /translation="MKVRYILMMALTVLTLLTITLWQTFRLGQEDGHNRYTQAQLDDY
                     SRTLKEAGTIISTASGVLRDVMTARQNRQQEGEIRREQLRNDITTDACARAVPDARFT
                     ERLHQHAERVRAAAVVPFHPGRADRPRTSPAVSGDAIMGKHRDMERQPHGRTGNL"
     gene            15637..16104
                     /locus_tag="KW011_001996"
     CDS             15637..16104
                     /locus_tag="KW011_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH7660329.1"
                     /translation="MLKTDSLREAMTRSCRWCHANPEKFTIFVESGNIETTGETPSFV
                     YRYQIVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRSSQGTPQAEHLPEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            16097..16732
                     /locus_tag="KW011_001997"
     CDS             16097..16732
                     /locus_tag="KW011_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HBH7660330.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGTAWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNN
                     IRTFYRDDIDRFLEIRTRRINQDSTRRVSMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            16729..17310
                     /locus_tag="KW011_001998"
     CDS             16729..17310
                     /locus_tag="KW011_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HBH7660331.1"
                     /translation="MSAELMRLLSNIIRTGIISEVDEESWCVRVRSGELETGWLRWNT
                     TRAGDFNVWFPPSPGEQVAIACIGGNPETAMIIGSLWSDAIPAPGKSLKEIVVSAPDG
                     AVFRYDADAGALSASGMKTATLQASISVTLNTPVVECTDLLRTATLDVTKGGTMSGNI
                     THSGGDFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            17328..17657
                     /locus_tag="KW011_001999"
     CDS             17328..17657
                     /locus_tag="KW011_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001705832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HBH7660332.1"
                     /translation="MNPDGTGNLNDMAHLKQSVRDILTTPLASRVMRREYGSLVPDLI
                     DEPMNNTTRLQCMSATVIALTRWEPRIALNAIDVVWKAGGHAGVTLSGTVMQTMQNVE
                     LTITLRE"
     gene            17661..18557
                     /locus_tag="KW011_002000"
     CDS             17661..18557
                     /locus_tag="KW011_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBH7660333.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIAAYPAEQREVVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEAARACMLSHSAGTDLDNLAGNMNTKRLV
                     ITPATDTTDVVMENDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            18550..19080
                     /locus_tag="KW011_002001"
     CDS             18550..19080
                     /locus_tag="KW011_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HBH7660334.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGTRITDIPADLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFNGEPGTFKIDVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIQGDIFA
                     AAATYDGEVITIYPDD"
     gene            19083..21053
                     /locus_tag="KW011_002002"
     CDS             19083..21053
                     /locus_tag="KW011_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH7660335.1"
                     /translation="MSTTTRKFKTIITDTGAKKLAQATAPDGKPVRLTHMAVGDGGGT
                     LPTPDSKQTRLVHEVWRHTVNRVTLDATHQNHIIAELVIPPETGGFWIREIGVFDEHG
                     DLIAVGNTAESYKPTVAEGSGRAQTFRTILTVSSTATVALTVDNTMVMATVNYVDDKL
                     KEHEQSRRHPDASLTAKGLVQLSNATNSTSETLAATPKAVKAVMDETNKKAPLNSPAL
                     TGTPTTPTARQGTNNTQIASTAFVMAAIAALVDSSPDALNTLNELAAALGNDPNFATT
                     MTNALAGKQPKDATLTALAGLATAADRFPYFTGNDVASLATLTKVGRDILAKSTVAAV
                     IEYLGLQETVNQASGALQKNQNGADIPGKDTFTKNIGACRAYSSWLNIGGDSQVWTTA
                     QFISWLESQGAFNHPYWMCKGSWAYANNKVITDTGCGNICLAGAVVEVIGTRGAMTIR
                     VTTPSTSSGGGITNAQFTYINHGDAYAPGWRRDYNTKNQQPAFALGQTGSTVGNDKAV
                     GWNWNSGVYNANIGGASTLILHFNMNTGSCPAVQFRVNYRNGGIFYRSARDGYGFEAD
                     WSEFYTTTRKPSAGDVGAYTQAECNSRFITGIRLGGLLSVQTWNGPGWSDRSGYVVTG
                     SVNSNRDELIDTTQARPIQYCINGTWYNAGSI"
     gene            21056..21589
                     /locus_tag="KW011_002003"
     CDS             21056..21589
                     /locus_tag="KW011_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBH7660336.1"
                     /translation="MMHLKNISAGNPETKEQYQLTKQFNIKWLYTEDGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVEVPDITANRRADISGKWMFKDGVVI
                     KRTYTGEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(21618..22145)
                     /locus_tag="KW011_002004"
     CDS             complement(21618..22145)
                     /locus_tag="KW011_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBH7660337.1"
                     /translation="MMHLKNIKAGNAKTVEQYELTKKHGVIWLYSEDGKNWYEEVKNF
                     QPDTIKIVYDANNIIVAITKDASTLNPEGYSVVEVPDITANRRADDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTAKPEWPQKPE"
     gene            complement(22147..23133)
                     /locus_tag="KW011_002005"
     CDS             complement(22147..23133)
                     /locus_tag="KW011_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH7660338.1"
                     /translation="MQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNNSDADTDSY
                     MWFETGNNGNEYFKWRHRIIGTRPKDLMNLKWNALSVLVEALFSSEVKISTVNALRIF
                     NSSFGAIFRRSEECLHIIPTRENEGENGNIGPLRPFTLNLRTGRISMGHGLDVTGDIF
                     ANRFAINSSTGMWIHMRDQNVILGRNAVSTDGAQALLRQDHADRKFMIGGLGNKQFGI
                     YMINNSRTANGTDGQAYMDNNGNWLCGSQVIPGNYGNFDSRYVKDVRLGSQQYYGVNN
                     WQTWNFQCPSGYVLTGINVQDTGKNSADNIAGVHYRPVQKYINGTWYNVASI"
     gene            23238..23825
                     /locus_tag="KW011_002006"
     CDS             23238..23825
                     /locus_tag="KW011_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH7660339.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGCEQIFEDKLSGTKTDRPG
                     LKRALKRLQKGDTLVVWKLDRLGRSMKHLISLVGELRERGINFRSLTDSIDTSSPMGR
                     FFFHVMGALAEMERELIVERTLAGLAAARARGRTGGRRPKLTKEQHEQIARLIKNGHD
                     RKQLAIIYGIGISTIYRYHPAGESIGTIEKSQETK"
     gene            complement(23861..24349)
                     /locus_tag="KW011_002007"
     CDS             complement(23861..24349)
                     /locus_tag="KW011_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH7660340.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDERIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LF"
     gene            complement(24362..27169)
                     /locus_tag="KW011_002008"
     CDS             complement(24362..27169)
                     /locus_tag="KW011_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBH7660341.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTGQYNNQLERERRALARVT
                     QARERYSRAQETAGKLKTGGALATGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     KNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLAKMGIRGSDAGTALRKSLANLSSPPKAAAEALKELGVSVADA
                     KGKMRPMENVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMGLVDAAGDGFLAKLR
                     KELEGARGEAERTAKVMANNLDGNLKSLGSAWEGLRIRISDLVDGPLRSVTQWLTRVV
                     SKVTALAQAHPALTRQLLIAGGALLATTATVGSLSLVIGVLHGKLATLRLGFSLLTES
                     MNVVRVLPALWGMVTGSVSLLGGAIGALFSPVSLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLTPLRETFERFGPVFDVVRDGIIQVFNWFKSLLSPMESSKETLDKCASA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDKPKDNKPLTDSNTGTLRRLSKIADNTGKLVD
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPVTSRNREPVASGFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGSFYDKD"
     gene            complement(27156..27311)
                     /locus_tag="KW011_002009"
     CDS             complement(27156..27311)
                     /locus_tag="KW011_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HBH7660342.1"
                     /translation="MCFEHIEDFVADIAAIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            complement(27320..27685)
                     /locus_tag="KW011_002010"
     CDS             complement(27320..27685)
                     /locus_tag="KW011_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH7660343.1"
                     /translation="MKETKNIDTENTVVADTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLNLKAESMFTLLSRVTSPRLDEVTIKKMASRDFIQLCVVA
                     VNFLSGADSGEKNEQATEA"
     gene            complement(27740..28252)
                     /locus_tag="KW011_002011"
     CDS             complement(27740..28252)
                     /locus_tag="KW011_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HBH7660344.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDAESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGRDIVP
                     DRIRSALGLG"
     gene            complement(28252..29436)
                     /locus_tag="KW011_002012"
     CDS             complement(28252..29436)
                     /locus_tag="KW011_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HBH7660345.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLPRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ASCHDCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVIGAIRAKLDSLVTAG
                     KLIGASCWYDVVDNGTTNLRQGRVRIRYKYTPVPPLEDMELYQSFTDEFFGPAFAVLG
                     GA"
     gene            29594..30703
                     /locus_tag="KW011_002013"
     CDS             29594..30703
                     /locus_tag="KW011_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HBH7660346.1"
                     /translation="MTDTTMQLLSQGTDPVKMPDFDILAEGKTLSGVAERLMSLSLTD
                     NRGFEADQLTITLDDADGQLQLPPRGARLTVLIGWKGEPLTEKGTYIVDEIAHEGPPD
                     RLTVSARSADFRAEFNVKREVSWHDVTVERVVSAIAHRYGLKPQISEMLMDIEIDHAD
                     QTEESDMSFLTRMAEMLGAITTVKSGNLLFIMPGGGVNAQGQPLPSFAITRSSGDRHQ
                     FRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPSKPKKEKSSSREGDYMEGAEGNVF
                     VLRKTYQNEQAARRAAAAKWQQLQRGAASFSITLAHGRAELYPEMHGTVTGFKSEIDN
                     QDWIIAKAEHTIDNSGFTTQLELEAKIPEWIAETE"
BASE COUNT         7706 a         8440 c         8084 g         6621 t
ORIGIN      
        1 cgtgcagatg acaaaataac gaggtgtcat tttatgctga agcaacgccg taatttttgt
       61 accggaacag aacgccacgc taaccgtttc actaccagtg catcacgcag caacatccgc
      121 tacagcctga gtgatacaca cgcaacgccg gatggctacc cagtaaaaca aatcggcgag
      181 cacgcctggc tgattgagaa agctggaatc gtgatccaca aatgcccacg caatccgttt
      241 accggaaacc gcatttttgc actgagctgt ggcgacaatc agttcgggca ggatttcaca
      301 ttatacgaag cacttcgcac ggttgatcgt ctgcttcgcg ggcaaagttt tattaaacag
      361 gctgatttat aacaggtgct ttatgaccaa agacaatgca caaggtgtat ttatccgttt
      421 tattgatttt cgcggtgaac tgttattacg tgcatccgct attgacggag tgactccggc
      481 gggtaaaaac ggagccgacg aagccactta cgtttatctg aacggcacgc gactgcttgt
      541 ggaacttccg taccagaccg tacgagaaat catcagcgaa gatgaaaagg cacgccaggt
      601 taatggcgat gaaccctata tcgaaattat ttgtatggat tcagaagctg aaatacagaa
      661 agcagattaa agggcgttgt gatgggcaaa gaatataaaa ctctcattaa caaagcactt
      721 gagcgttttt attttcgctt aagtgcatca ggcgctcatg ctgaacgtgc ggcccgtgac
      781 tcattgacca gagcaatccg aagtctgtat gacgtggctt tttacgctga tgatctggat
      841 gcacttaacg aactttccga gctgatctgt gccgcagaat gcggggaaca tattgaaccg
      901 tataagctgg gaaatatcgc atgagtatat ttatttcatg gcttgttctg attatttcgg
      961 tggtctgcgc cattgggatt atgcgaatta ttaattcagt aaaaaagatt gaacgctttt
     1021 tcactgaaga ataaccgcgc aaataagacc ccaggttaaa taagaaaatg tgaaaacaat
     1081 ccgcattcgc ggaggtattc gcacacgcca aggaggcgta atggcaatta agcattttcc
     1141 tgtcgttcgt ttcacctcca gaggacgtga atacgaagtt gacgaacgcc tgattaccac
     1201 aatcgacaaa caccgttcag aaaaggatgc acatcatatc tatctcactg acggcactta
     1261 cttctgcgcc accaacgtgg tgcaggtgaa tcttatcaga caggtacagg agtcacgcag
     1321 atgaccattc tggactacat cgctgccaat ccgggttgta gcagtggaga aatcgccgca
     1381 gcactgaata caccaaccac aaccattaat gcggagctac gccgtctctg gcgcagcggt
     1441 ttagtcacaa gaaaagagcg caaaacaggc ggtcgctttt cttatcaggt aaacctgatg
     1501 ccgtttgggt gtagcaaccc actaacccag atgttcaacc agctactgag ggaaatcaga
     1561 gcatgagcac ctccaactgc cggaaaccac gtcgggcttc agcagctcat ccggcagcaa
     1621 aacaaactcc attaattcct gttccgggcc tttcctgcac cttgcggcgg gaggccttcg
     1681 cacatctgta acaagaggat tgccgcaatg attctcgcca acgactttct tgaatacctg
     1741 ctcaacacag agcgtgatct tgccgctcgc gtgcgtgatc gttatgacat gtacctgaaa
     1801 tccctgcctg taccgcagct cgctgacgga aagattgtta ttgatggtcg ctacatgatt
     1861 gacagccacg agggaaatta caggctttac cgcattgaag gtggcacccc gtccgttatt
     1921 ggcatttacc agcgcccatc ctctgcaatc gtcgatgtga ttgccgacag catccgcatc
     1981 acacatcgcc atgccgacac agaagacacc gtgctggaaa ttcagcggct ggctacagtc
     2041 tgccgcgaca ccctgaatgg catgacgaag taaatcacta tgacggcaga gtacatcagg
     2101 gactggcaac aaccgcgcca cgcagtgggg cgtgaaggaa cggggatccc cgctcctgaa
     2161 tccgcgcttt cctcctggct ggatgcccac cgggcagaga acgagcgccg ccaggaaatg
     2221 gctgatgcgg cgttctccgc cacgccactg ggcaacctga ttaataaaag cctggacgca
     2281 caggaaaaac aggacaaaac catcacactg gcaggagacg ccagaaaaca ggcacgcggc
     2341 gcggtggatg aagccatggc ctcgctgcgc ctgctgccgt cctatctgcg cgatccgctt
     2401 attcgccacc tctccttcct gcgcaaaaaa caggaagccg atcgccggaa aggcaaaaag
     2461 agctggcagg cggaacgcta tgcacgcgga accctgcgca aaatattcga acgtctggat
     2521 cgcactgacg gacactggct gacaccgggt tatcgctccc ttgccggacg tgaacgcctg
     2581 gacgatttgc tttacctgcc gcaactcaac aaacaccaga tacagacgct ggccaccatg
     2641 acggcggcga tgttcagcag caccttcgaa aaactctgcg atggttttgg cgcgactgat
     2701 ggcgagctga ccatggatgt aacgctgaag gcgtatcaga tgctggcccg catggcgtta
     2761 cacttacacg ccatgcctcc gcattatgac gcactgacaa cagacaaaga ccggaggcac
     2821 gaaccggaca cagaactgct gccgggcgca atccttcgcc tgacctgtgc ggaatggtgg
     2881 aaacgcaaac tgtggctgtt acgttgcgag tggagagaag aacaacttcg cgccgcctgt
     2941 ctggtttcca gaaaaacatc gccctatctg agccaggacg cgttaagcga gtttcgcgca
     3001 cagcgcgaga aaacacgcga tttcctgaaa agtttcatgc tggaaaatga agacgggttc
     3061 acgattgatc tcgagacggt gtattacgcg ggagtaagta acccggttca ccgtaaggca
     3121 gaaatgatgg ccaccatgaa ggggctggaa cttctggccg aagcccgtgg cgacaaagcg
     3181 gtgtttctga ctgtcacctg cccgtcaaaa taccacgcca caacggagaa cggtcatccg
     3241 aatcccaaat ggaacggggc cacaatgcgc gactccagcg attacctggt taacacgttt
     3301 tttgcgacgg tccgcaaaaa actgaaccgc gacggcctgc gctggtatgg catccgcacg
     3361 gcggagcctc accatgacgg caccgtgcac tggcatatga tggtctttgc tcatccggaa
     3421 gaaatcgaca ccattgtgtc ccacacccgc gatattgcca ttcaggaaga tcgtcacgag
     3481 ctgggcgatg acataactcc gcgctttaag gcggagtatg tagacggctc aaaaggcacg
     3541 ccaaccagct acatcgccac ctacatcggg aaaaacctgg acagccgcgc cgtggatggc
     3601 atcgacccga aaacgggcaa gccacgcgtt gaccacgaaa ccggaaaatc aatggccgag
     3661 agcgtggaac gcgccatcgg ctgggcgcgc cttcaccggg tccgccagtt ccagttcttt
     3721 ggcatcccct cccgccaggt atggcgtgaa ctgcgccgcc ttgccagcca gatggcacgc
     3781 aacccggaag gcccgcaacg gctgaaggat gacgcaatgg atgcggttct tgccgccgct
     3841 gatgccggat gttttgccac ctacatagag aaacagggcg gcgtacttgt tccacgcaaa
     3901 gactacctga ttcgcaccgc ctacgacctc gccgatgagc tgaacgatta cggcgaacag
     3961 agcgtacaga tttacgggat ctggtcacca ctcatcgggg aatcctcccg tgtgtgcacg
     4021 catccggata actggaagct ggtaagacgc aaaccggaag cggaagacag cgcccgcgaa
     4081 aatggttttg accttcaggg cggccctgcc gccccttgga ctcgtggcaa taactgtccc
     4141 cgtgtacagg aaacggacaa caacgggaca gaacagccgg aagaacggcc agcaccgtgg
     4201 ccgcagctcc ctgacggcgt tgatgtggat gaatggatgc gctcactgaa acggcacgaa
     4261 cgccgggcgc tgatgcgttc gcttcgtgac aaacaggcaa aaaacagcag tgatgaaatg
     4321 cagagctgga cacagagccg caaacagcag cggcctttgc ctgataacca cgaattactc
     4381 gctaaagaat ggcgggagtc tgctgaatct ctcggcctgc atatcggtga acaacagatg
     4441 cagcacctgt tacggggcgg cagtctgtac gttgacggca gcatcattgc accgcaggga
     4501 tttgaaattg tacgcaaacc ggatacccgc ccggacagcc gaatcacgca gctctggcag
     4561 cgcctgagcc gtaatcatgg cgtaagcagc acggagatcc gccataaccc ggtcgccagc
     4621 tatctgacac agctgggggc atcagaccct gaagccgccg cacgcctggc atccacactt
     4681 cagcaggacc agaacaccat gaaaacaccc gttaccgtgc tttctgacat gctgcgcgcc
     4741 atccgcgacg cagagcacgc acagagaatc agtgaaacca ctgaacgcgc ccgccgcaaa
     4801 gcagacctgc tgcggggtgg cctgaccagt ggaaacaaaa aacagacaga aacgggactc
     4861 acgaatcccg taaatgagca aaaaacgcgc agcgatatat gaagcgcgca caaaacaggc
     4921 aaaaacggga tttcagaatc ccgtaaacga ttaattaatc aacataagga aaagcgacat
     4981 gaaaatttgt atcgacgacg gctccaccaa catcaagctg gcatggactg agaacggcga
     5041 acgccgcaac gccatcagcc cgaacagctt caagtcggaa tggtctgcgc cgttcggtgg
     5101 catgcagccc gcgaactaca tgcttgatgg cgtgcgctat ggttttgatc cggtcagcga
     5161 tcgctttgtc cagacaaccg acacgcaata ccagtacagc gatgtaaatg tcattgccat
     5221 tcatcacgcg ctggtcaaat caggcatcac gccacaggag gtggatgtgg ttgtcaccct
     5281 gccactgagc gagtatttcg acacaaacgc acagccggac atggccaaca tcaaccgcaa
     5341 aaaagcgaac gttatgcgcc cggtggagta ccagaacggc gaagcattca ctatccgtaa
     5401 cgtgcgggtt atgcctgaat ccattccggc tggctttaag gcactggctg acatgagtcc
     5461 gtttgaatcc ctgctgattg tggatttggg cggaaccacg ctggatgtgg caaaggttca
     5521 ggggcaactg gcaggtatca gccaggtgtt ttgcgatcca cacgtaggcg tttctctgat
     5581 ggccgatgcc gtactgtcgg tgatggccac taacggtatg cgcaccagtc accacatcgc
     5641 caataccatt atcgaacatc gccatgatga agcctggctg cgccagcaca tccacaatga
     5701 cgcgcattac gccagcctga tggcggttat tcgtgaaaag gaagaaacac tgaaacaacg
     5761 cgtgatccgc gcgctggcgg gtttttcggg ttacgggcgg gtgatggttg tcggtggagg
     5821 ggcggagatt gtggcacccg ctatccgcga agcctgcgga gttaatgcga ctttcatcgc
     5881 ggacggggtg ccacagtttg ctctggttaa tgggctgtac gcaatggaca aggagtaaac
     5941 caatgacgac accaaccaga cggataagtt tctatctgaa gcctgcggcc gtcaagaacg
     6001 aaagcgaagc atgcgcctgg ctgggcagcc ttacaccaga agcccgcaaa agcggccaac
     6061 gcgtggcttt tctggccggg ctggcacttc tgaaaatgaa tccagcagag gcttaccgac
     6121 tggctgcatg ggctgacgat gaggcgttat cggtgacaca aaccaggaca gaacgccccg
     6181 tgccacagcc agtaccaacc gcacagataa ccagtcagat agcagggaat atccgggcat
     6241 tatttcctga atgacgaatt tgacggcttt taattttatc ggggtgcact gtcgccccgc
     6301 acgaggaaaa cacgatgaaa aaatacgaat acatgctaat gaaagaagca ctgcgggctg
     6361 gtctggctcc ctgtcctgta tccgtagaag ctgttgttgg gaacacgatt ttccgaacgc
     6421 tgcgaaatcg cgtatccgtg aagatggttc accaaccacc caaatctacc agcatgatct
     6481 atctgccgct gcccgaggct atcaaactta tgcgttcgct gaaatttgcg atttggatgc
     6541 agacacaaat caacaaagcg gtgcaaaaca ccttcgttaa gagactaata ccggagcttg
     6601 ctgccagatg tgcaaaatgt accagacccg cacttcaatt tgatctgtca ttgattcaac
     6661 aagctacgga ggcagaactg taccgcctgt cacctatccg tgatgctcac acggaagcat
     6721 cagaaatgtt ccggctcatg tttcaattta attttgccgt tcgacgcaca cgcgttcagg
     6781 tcaatcgcat gatgcaggag caggaagagt taacacagta caaagggggc agccagtgag
     6841 taattcagca cgactacagc ttggtttttc accgctatca aaaaccatcg tattggcaaa
     6901 aatgcgtgac ttaggagacg gaacaaaacg ccgcgtagga aatgatcgcg gtcgcgatgt
     6961 aaccaacgag gccgctcaac tcgtttggca tctagtcatg gcagaaggcg gtgaaattaa
     7021 ttgggagctg gatgatggtt ctcgtatgat tttgagggcc gagaagagta aatagctcaa
     7081 gggggattaa tgctaaatga tgatgaagaa gagctgctta tgcaggaatg gtcgctcggt
     7141 gattatgata atggtgaaaa tgggtgcccg cattgtggcc gttatcgttt atgtatctgc
     7201 cagaatggaa aacacagatg tgaaaagtgc aattggtctc cggaacttaa tgactacgtg
     7261 ccgatcgagt ggtagaggta agtcacacta ggtgtttttt agttgatttt ataaatgcac
     7321 aatagtgcac aaatttgcac aatttttttg aacgactttt tgcccttccg gcccgcgtgg
     7381 cggctggatc cgtcaaggat ccgtgcatgc acaaaaaaac gcgttttttc tgcgcgcagg
     7441 tgacggggga acagcccgcg tttcaggggg taaatagcat tccctgaacg atgtcgcaga
     7501 gatacaacag aatggctgta tttctcacgc tgagcgtgaa aaagacgtga gggcttttga
     7561 tttgatgggg tgaaaggtaa ggccgtcaaa atcgcactga gacggcgaga atatgcagtc
     7621 aacgaggtgg gattgcgtaa gagtctgact gtcgatgatg gcaatcagca ggaaagcgtc
     7681 gtgaaattat ctgactgata caggagctgg agagtcgggg cataaatttt ttatgccccg
     7741 gcgaagcagc agacaagcga agcgcgtcag gatgtgggct gggtgtctaa cagtgcgtaa
     7801 gggttaaagc ggatcacctc ttcgccaagc cagtcattga tgtgcttcat ggcctccatg
     7861 acgggcatca gctcgttaat tgcgtaaacc cgcgcggcct tctccacatc accaaacgca
     7921 cttttttcgc ccggcatcgc ccccatcagt tgcggcggaa cgcggtgcgc agccagcaca
     7981 tcatcacggg atgccgcctt aacattcatg aactcatcct ttgcggtgat ctgctggaac
     8041 ggcaaaattt gcaccccctc tttgcccccg ttgggcgcat gaatgagcac gtttttaaac
     8101 gcaccaccac cacgtgcccc ctgtagcgtt tctttcaggg agtccatgct ttcgcggttt
     8161 acctgcgctg caccgatgta gatgatgcac ccggcgtggg atccgttgtc gtagtacagt
     8221 tttctgaaca tgtccgccga atgagaaagg ctggccgaga gtaatgcgcc aagatattcc
     8281 ggcatgccgt agatttcctg gttaatatcc ggattcatca ggtggcacac tttgccagga
     8341 cgaaactgaa acgcatcctt gccatcctgc acataccacc atgattcaag atcgcttccg
     8401 cgtcgcatgt atttcgccag ggcgtgccgt aatttaagcg gttcgccgag catattgctc
     8461 cgaagctcaa ggaatgcgtt accgaacaca aaccagtcca gcgccagcgc cgagaaatcc
     8521 tgccgggaaa gcagcgggtg cggaatatag cagccgagca atacattgcg cttaaagtaa
     8581 agcgcagact gatgccagga cgtttgccgg gcagctcttg ccagaccgta ccagtccacc
     8641 ggggtttcat accaccgccc gttatcagca cagtacatat tgtccagcag gtcatgcccg
     8701 gtcaggcggt aaggaccatc aaatgtgaat gcactgagcg acgattcttt cctgagcgca
     8761 tcagcgagat caatgcgtga actcatgcgc acttttttat tttttctgct catcagaact
     8821 ccataaccgt gaaacgctcg ttttctcctt cgccgccaat cggttcgtta atgacagcaa
     8881 gcatggttgc ccacgcaagg tcgccgtggc tgatccccct cgcgcggtcc gtttcgtaag
     8941 tgataaaacc gcccggtgtt ttcaccttac gcacggcgtt aaaggccgca accagctcgc
     9001 gttcggcgcg atcgtattcc caccgcccgg cacgcattat ttgcagcatt ttcagtacca
     9061 gcgaccgttt tgatgacagc gtgaaggtgt aagggatggc ggcgggaaaa aaccgtttca
     9121 ctatctgata gacggcatcc ccgtgcccgc ccgtcgcatc aatgccgatg tgttccacgt
     9181 tgtagcgata cgtgaactct tcaataactc tggcctgctc ttcaaattcc agcccctgaa
     9241 cgcgtcgcgt ctccaccgtt ctgaaacgac caccaggaac agccggagga accaccacgg
     9301 acacagcgcc gctgtcgccg ttaccactgc tgccgtttgc gtcataccca atccataccg
     9361 gacgattccc catcgggcgg ggggcaaagg gtttccagtc tttccagtcg tcgtatccgt
     9421 caacaccgca gccaatcagg atattcaggt taaatgccga ttccccttcg cggacaaact
     9481 cacacatata gagattgcgg aactcgtctt cggtgttttc atcacgaatt tcatcaatat
     9541 cggtgtgttt ccagccgtga ttaaccacat cttccagcgt gacaatttgc cgccacgtcc
     9601 ggtcagggca gataagcccg ttatgcagcg ttttccagtc cacagaaaaa cgctggcgtt
     9661 tatgcgcggc ctttttctcg ttccagcggt cgccgttcca gtaggcgtat gcctcgtgcg
     9721 tttcggtgga tggcgtggag aagcaggtgc gccgcagtcc gctgagggtt gccatagcgc
     9781 cagccacctt gcgcagttca gcaaagcgac tgacccagaa aaattcatca aaataaaaat
     9841 tgcccgtgta agattgcgcc gtcgcagcag aagtgccgag aaaatgcatc tctgcgccgt
     9901 tggagaggat gattttatcg ccccctttca gctccacatc gacttcagcc gcggccttct
     9961 gaataatgct tttaaactgg aacgcctgac gacgcgacgc agacaaaaaa atctggttac
    10021 gctggtaagg ttgtgccaca tcgtcacgca gcgccatcag cagtgcttcc tgtgcaaaat
    10081 accaggtcgc cccaatctgt cgggatttca ggatcatcct gttacgtatc ccggcttccc
    10141 tgcaaagcgt cagggagtca aaccagcccc gctgatgcca ctccagcctg ctgatgattt
    10201 tttcccgcag tgcggcaatc tgttccggcg tgaaatgatt tttgagtttt ttcgcccggc
    10261 ctttctttcc tgtggccgtc gcatccggct ggccatcatg cagtttttta agctgccggg
    10321 tcagcaggtc tatttcctta aagtcaccac ctgttttatt ctgtttttca gtaagctgga
    10381 tgaggcgcgc atcgatggac tgcgtgacac gctgcacggg tggcgtttca tcccactggt
    10441 cgcgtttttt ccacgcataa atcgtgttcg ggtttattcc catcagacgt gatatttctg
    10501 cgggcggata accctgccag taaagttgcc gcgcacgctg gcgcacaaaa gcgtcctgaa
    10561 tcattgctcc ccctgagtaa ttacaggaag attacccgcg cgcgaaaccg ttctccttaa
    10621 ccccctgttc tggccgtttt cttacaacaa aagccctttg tatcagcctg ttacgctttg
    10681 ccatcatgac tgaagaacca gtcagagggg caaaaactat ggctaatgaa aaaaagacat
    10741 cccgcaaaaa gtttcgcgtg gctgtctccg gatcaactgt tgatggccgt gaaatcagtc
    10801 cggtgcatct gcgtgaagcc gccgagaact tcaacccgga tgtttacgct gcccgcgtga
    10861 acgttgagca ctatctctcg ccatgcccgt caagcgaatt ttccgcaatg ggcgatgtca
    10921 ccgcactgag tacggaagac attacggaag gtccgctggc cggacgtact gcgctgtatg
    10981 cagaaatcga accgaccgag cgcatgaagc agcttgtcgc ggacggcaag aaaatctatt
    11041 ccagtatcga actgcacccg cagttctccg ttaacgggcg cgcctatctg gtcgggctgg
    11101 cgatgaccga caccccggca agcctgggca ctgagcgcct gaaattcacg gcacagcaac
    11161 gtcaggcggt gatgacgttc aacagtatcc agggtgaagc accgcttatc tccgaagcca
    11221 tcgagtctga aatcatcgaa atggcagaac aacgccagga agaaggcacc cagtggttta
    11281 accgcgtaat ggggattatt ggtcgtggcc gcaaagcgga tgacgccagt ttctcccgta
    11341 ttcaggaagc ggtggaaagc gtcgcaacgt cacaggccga cattatcgac cgttttaatg
    11401 cgctggaaac acgccatcag caggacagcc agaaaatcac tttactgacc acggagctga
    11461 cagcactgaa ggaaaaactg cgcacgcagg acggcgatcc gcagaaccgg ttcaccgcaa
    11521 cgggcgcagc ctccgaccag ctggctgact tctgataaga caaaggagca aattttttat
    11581 gaatctggtg atgtcagata ttacccgcaa caagctgggt tgctatatgg cgcagcaggc
    11641 gtcgcttaac aatatcccgg tttctgcact ggtatcgcga tttaccgtgg aacctgcggt
    11701 gcagcaacgt tttgaaaacg cctcaaagga tagcaccgaa cttacaaaaa aaattaacgt
    11761 gatcggcgtg accgaccaga aaggcgaaaa aatcctcctg gacaccacag gaccgattgc
    11821 acgcacaaat accagttatg acggaacaaa acgccgtaac ccgaataacg tggttgatct
    11881 gaaaaaccgc aaataccagt gcgaacaggt gaactacgac acgtttattt cgtatccgca
    11941 gcttgatgcc tggtcggcac acagcgattt tcagtcccgc atcagcgcac agattgcccg
    12001 acaggtggca cttgaccgca tcatgatcgg tttcaacggc acatctcacg cggatgagtc
    12061 caacttcagc accaacaagc tgcttcagga cgttaacgtg gggtggctgg agcacatcag
    12121 aaccaatgcc agcgagcgcg taatgaatga cgtgacgctg acttcccgca acatggacaa
    12181 caccgtggcg cacgcgggta aatacgcgaa ccctgatgca ctggtacagg acgcgcgctc
    12241 atctctgctg gatgaatggc acaaggaagc tgacgacctc gtggtgatta tggggcgcaa
    12301 cctgtttaac tcgctgcgtc tgccagtgct gaacagcatc agcggccaga atcccaatgc
    12361 ggaattactt gccgggcagc tcatcctgtc atcgcgcacc attggcgggc tgggtgtgtt
    12421 ccttgcaccg ttcttcccgg atgcaacgat gctgatcacc tcattcaaca acctgtcgat
    12481 ttactggcag aaaggttcaa tgcgtcgtct gatgaaagac gaaccggaat acaaccgcat
    12541 cgccacctac cagtccatca atgacgctta tgtcgttgaa gactatggca agtgcgcgat
    12601 ggtcactggc ctgaagttcg ccgacagcta atcaactcac ggcgggcatc atgcccgcct
    12661 gtaacggaga gaaaaaatga ttactcctgc acagcaacac tggcagaacg tgatggcaca
    12721 gcgcgcaggc cgggcgaatg aaggtgtgga ccacgccgcg cgtaccgcgc atgaagaggt
    12781 gctgtatcgt ctgcgtctgg cacaggcccg gcttaagggc gtacaggcca gaagcgcgaa
    12841 agccgacatc aaaaaagcgt tgttgccgga cttttccggc tggattgagg gaacgctgga
    12901 ggctgacggc gggcaacagg acgaagtgat tgccacgctg atggtgtggg cgattgactg
    12961 cggcgatctt ccgcttgcgc tgcgtattgg tgcgtatgcg gtccgtcaca acctcatcat
    13021 gccggataac tttggccgta ctgctgccac agtgctgacc gaagaaatct gcaatccggt
    13081 actgacgcag gccgggacgg atgccgacgc ggatttgtcc gtctttatcg aaccactgga
    13141 cacactttgg gaaattgtcg ccaaccagga tatgccggac gaagtgcgcg ccaaattatg
    13201 caaggcgtgc gcctttgccc gccgtggcct gagcgatgcg gacagcatgg cctcatcact
    13261 gaagctgctg cgcgaagcga tgcatctgaa ccagaacgca ggtgtgaaac gcgagattgc
    13321 aaccctttcc cgcgccctga aaaaagccga ttccgcagcc gcaccagaag acgccagcgc
    13381 acagcaggcg caggacgaaa gcagcgaaag taaaaagaca acgcggaagc ctgcaacacg
    13441 aaaaaccacc gcgacgcaga aggcgaagcg cggttaacga ctgaccccgt cagcgggcgg
    13501 cgtgcgcggt gttccggttt ggctccgtga ccgtttacac cgcgcaccca ccgcccgatt
    13561 ttttcaggag tgaaccccat gagtatggtt gccagaactg aacccggacc cgcagaggac
    13621 gacatcaccg acaccgaaga tggcgatacc cgtatttcag cgggtgcatt ctggccggat
    13681 attgtgctgc gcgagctgcg tctggcggta cgactgccgg gccgcgtgac cacctcccgc
    13741 ctgctgcata ccgccaccgg ggccgtggca cacgttaccc gcgagctgga agcatggcag
    13801 caggaacagc aggcggctgg ccatcagacg ctggccgatg ttccggcacc tgtaattaac
    13861 ggagaaagcg tcaatctctg gcactggcgc aatgctgttt ataccgccac acgcgccctg
    13921 attctggagc gttaccgcga tgcggacaca acggacaagg gcgaccgccg ggcggacgca
    13981 ctggatatac agacatcgga tttgtggcgc gatgtgagct gggccatctc tgacattctg
    14041 tgccgcccgc gaatctttgc ggagttgtgc tgatgaaagt gaagacactg gaaggcgaca
    14101 ccgtggattc gctctgtttc cggtactacg gcacgacgca gggcgtcacc gaaaaggtgc
    14161 tggatgccaa ccccggactc tgccgacagg tatttctgga agccggacag gaggtggaaa
    14221 tgccggagcc ggagaagaaa aaacaacgag agatgataca gctatggtaa caggtttttt
    14281 tcagcgggtg agtgactgga tcaccttctg ggcatcagtc acagccacca caataggcgt
    14341 gatgaccatc agtgaaaaaa tcgcccttgc cgggttgtta ctcggttctc tgtctgcatt
    14401 acatgcctgg cgacatcgcc gcctgatgga aaccgccatg cagcgtcgca atgaactcat
    14461 cgccagtatc ctggcgcagt cagacgcacg gccacttcac gacagtgagc gtcaggctct
    14521 gaacattctg cgggataacg atacacatga taaagctgcc cgttaaacgt tacgccgtag
    14581 ccgccattgt ggcgctgggg ttctccattg cccctcacaa actccggacc agcccggaag
    14641 cgcaaatccg cattgccaca tgggaggact gtcgcgcaac gccttaccgg gatattgccg
    14701 gggtaatgac cgtggggtgc ggctcgaccg ggcatgtgga aaatcgtctg tacaccgaaa
    14761 cggaagtcgc cgggcgctgg gttaatgata tgcaacatgc agaaaactgc ataaatcaga
    14821 acttcagcgg caacgccatg ccacagtccg cgtttgaggc aatgacagat gccgccttta
    14881 atcttggatg cagaaatctt atgtggttca gggataagaa ccgtaactac caccgcacca
    14941 ctatctggaa acatgcacag gcgcacaact ggcctgacat gtgcaacaga ctgactgact
    15001 tcgtgaacag cggcggcgaa cgctcacagg gactggttaa ccgtcgtact gattttcgtg
    15061 catggtgctt acgtgacacc ggaggccagt catgaaagtg cgttacatcc tgatgatggc
    15121 actgaccgtc ctcacgctgc tgaccataac actctggcag acattcagac tggggcagga
    15181 agacggacac aaccgttata cacaggcgca actggacgat tacagccgga ccctgaaaga
    15241 ggccggcacc atcatcagca ccgcatccgg tgtactgcgt gacgtcatga cagcgcgcca
    15301 gaaccgacaa caggaagggg aaatccgacg tgaacagcta cgcaacgata tcacgacaga
    15361 tgcatgtgcc cgcgctgttc ctgacgctcg ctttactgag cggttgcacc agcacgcaga
    15421 acgcgtccgc gccgccgccg tcgttccgtt tcatcccgga agggctgacc gcccccgtac
    15481 ttcccccgcc gtttccggtg acgccatcat ggggaaacat cgggatatgg agcgacagcc
    15541 tcatggacgc actggaaacc tgtaacgccg ataagcgggc cattgaatta ctggaacagc
    15601 gcaggctgca acgactgaac aacgaggaca acaaccatgc tgaaaactga ttccctgcgt
    15661 gaagccatga cccgttcatg ccggtggtgt catgccaacc cggaaaaatt caccattttc
    15721 gtggagagcg gcaacattga aacgaccgga gaaaccccat cgtttgttta ccgctatcag
    15781 atagtgatgt ttgtcatgga ttacgccgga gagctggacg acctcacgct gccgctgctg
    15841 gcgtggttat ccgaaaatca gccacagttg ttgctcaatc cggagcgtaa tcaggacatc
    15901 aaattttccg ccgttatcaa tgacgatgac agcgccgatc tcctgtttac gctccccctg
    15961 cgggaacgcg ttcgcatcac gcgcagcagt cagggcacac cgcaggcaga acacctgccg
    16021 gagccaaaac cccgcctgcc ctcttccgaa ggcgactggt cgcatgtatt ccaggatgtg
    16081 acgtgggggg aaagcgatgg ataaggcatt cacccgcgtg gatgaaacct ttgaggctat
    16141 ccgcgacagc ctgaatcagc aggccattaa taacatcgcc agaaagctgg cacaggattt
    16201 acgccgcgcc cagcaggcgc gtatccggtc acagaaagcg ccggacggga ccgcatggac
    16261 accacgcaga cgccgcgtaa cccggataca ggagcgcatt cgctttatct ggaataacga
    16321 agcacgcacg ctgaaaaact ggcatcacga cacggggaaa tacgggcgaa ccatcaccgg
    16381 gtgggatgag gataaaaaca atatccgcac gttttaccgg gatgacatcg accgttttct
    16441 ggaaatacgc acccggcgca tcaaccagga cagcaccagg cgcgtctcca tgttcgtaaa
    16501 actgcgcacc gcccgctacc tgaaagcccg tgcagatgct tccggtgtga cggtgggtta
    16561 cagcggcgtg gccgcacgta ttgcacgcgt tcatcagttc ggtgagcgcg atcaggttgc
    16621 gccgggcatt ttcaccgatt acccggtacg tgagctgctg ggtatcagcc aggcagatga
    16681 gcgcctgatt tataacacgg tgctgggccg gattgcggag gctgtacgat gagcgcagaa
    16741 ctcatgcgac tgctgagcaa tatcatccgc accgggatca tctctgaagt tgatgaggaa
    16801 tcctggtgcg tgcgcgttcg cagcggcgaa ctggaaacag gctggctgcg ctggaacacc
    16861 acgcgcgcgg gagacttcaa cgtgtggttt ccgccatcac caggcgaaca ggtggcaatt
    16921 gcctgcatcg gcggcaaccc ggaaaccgcc atgataatcg gcagcctgtg gagtgatgcc
    16981 attccggcac ccggcaaaag cctgaaagaa atcgtggtca gcgcgccgga cggcgcggtg
    17041 ttccgctacg acgcggacgc aggcgcactg agcgcaagcg gcatgaaaac ggccactttg
    17101 caggcatcca tcagcgtgac actgaacacg cctgtcgtgg aatgcacaga ccttctgaga
    17161 acagcgacgc ttgacgtcac aaaaggggga acgatgagcg gcaatatcac gcacagcggc
    17221 ggcgatttca cctcaaacgg catcacagtg catacgcata agcacggtgg cgttaaaggt
    17281 ggcagcgatt cgacaggagg cccgcagtga caacccgcta cacaggaatg aacccggacg
    17341 ggacgggaaa cctgaacgat atggcgcacc tgaaacagtc agtcagggac atcctgacca
    17401 ccccgctggc aagccgggtt atgcgacggg aatatggcag ccttgtgcct gatttgattg
    17461 acgaacccat gaataacacc acgcgtctgc aatgcatgag tgctaccgtg attgcactga
    17521 cacgatggga accacgcatt gccctgaacg ccatcgacgt tgtctggaag gcaggaggcc
    17581 acgccggggt gacgctgtcg ggcactgtca tgcagaccat gcagaatgtt gaattaacca
    17641 tcacgctgag ggagtaaatc atgcctgccg ttgacctttc ccagttaccg gaacccgcca
    17701 tcatcgcgga gcctgacttt gaggcaattc tggctgacac aaaggccatg atgattgcag
    17761 cttatcccgc cgaacagcgt gaagtcgtct ccgccgcact ggaactggaa tcggaacccc
    17821 tgaacgttat cgctcaaacc atgtcgtttc gtgaaatgct gttacgccag cgggtcaatg
    17881 aggccgcacg cgcctgcatg ttgagccaca gcgccggaac agacctggac aacctcgcgg
    17941 gcaatatgaa cacaaagcgc ctggttatca ctccggcaac ggataccacc gacgtggtga
    18001 tggagaacga cacctcgctg agactgcggg cgcagcgggc gtatgacggc ctgagtgttg
    18061 caggcccgtc aggcgcatac gagtattttg cccgcagcgc cagcggtctg gtgcgcgatg
    18121 cgcgggccat cagtccgtct ccggcaaatg tgacggtttc catcctgtcc actgagggcg
    18181 acggcacagc aacggaggcg ttgcttaata ccgttcgcgc cgttctgaat gcagaggata
    18241 cccgcccggt ggccgaccgc ctgaccgtac agagtgccag aatcgtgaca tggcggctga
    18301 atgcaaaact gtacttttac cccggtccgg aatccgaacc tattctggct gcggctgaat
    18361 cgtcgttcag gaagtggctg gctgagcagg ggcttatcgg tcaggacgtg gcgttgtccg
    18421 ccattgctgc cgcactgcat gtgcacggtg tgcaacgcgt ggagataatc gaacccacac
    18481 agaatatggc catcagcgac atacaggcgg cgcgctgtga gtcattcacc atcagcgaag
    18541 gtgggcgtaa tgagtaattc actgttacca ccatcagcca gcaatttcat gcgttgtgcc
    18601 gaagccgtcg gaacacgcat tacagacatt ccggcagacc tcaacacgct gtggtcgccg
    18661 gacacctgcc cggtgcatct gctgccttat ctcgcctggg cgttttccgt tgaccgctgg
    18721 gatcgcaact ggccggaaga gacaaagcga caggtgattc gtgatgcatg gctgatacac
    18781 cgacacaaag gaaccatcag cgccctgcga cgggccattg agccgctggg atacctcatt
    18841 cgcgtgtctg agtggtggga gttcaacggc gaacccggaa cattcaagat tgatgttggc
    18901 acgctggaca gtggcgtgac ggaggaaatg tatctggaaa tggagcggtt gattgctgat
    18961 gcccgcccgg tcagccgcca catgacaggg ctgaatatca ttcaggagat tcagggagat
    19021 attttcgcgg cagcagcaac ttacgacggt gaagtcatta ccatttatcc ggacgattaa
    19081 gcatgagtac cacaacacga aaatttaaaa ccattatcac cgatacgggt gcaaaaaaat
    19141 tagcgcaggc aaccgcgcca gatgggaaac ctgtccgcct gacacatatg gccgtgggtg
    19201 acggcggcgg tactttaccc acgccagaca gtaagcagac ccgtcttgtg catgaggtgt
    19261 ggcgacatac cgttaatcgc gtcaccctgg acgcaacaca tcagaaccac attattgcgg
    19321 agctggttat tcctcctgaa acgggcggat tctggatccg ggaaattggt gtgtttgatg
    19381 agcacggcga tttgatcgcg gtaggcaata ctgccgaaag ttacaagcca accgttgccg
    19441 aaggatccgg acgtgcacaa acatttcgca ccattctgac cgtatccagc actgccactg
    19501 tggcgcttac cgtggataac accatggtga tggccacagt gaattacgtg gatgacaaac
    19561 tgaaagagca tgaacagtca cgacgtcacc cggacgcctc gctgaccgca aaaggccttg
    19621 tccagctcag caacgccacc aacagcacgt ctgaaacgct ggcagcgaca ccaaaagccg
    19681 ttaaggcagt aatggatgaa acgaacaaga aagccccatt aaacagcccg gcactgaccg
    19741 gaacgccaac aacaccaact gcgcgacagg gaacgaataa tacccaaatc gcaagcacgg
    19801 ctttcgttat ggctgcgatt gccgcacttg tagactcttc gcctgatgca ctgaacacac
    19861 tgaacgagct ggcggcggcg ctgggcaacg acccgaattt tgcgaccacc atgactaacg
    19921 cgcttgcggg taagcaaccg aaagatgcca ctctgacggc gctggcgggg cttgctactg
    19981 cggcagacag gtttccgtat tttacgggga atgatgtcgc cagcctggca accctgacaa
    20041 aagtcgggcg ggatattctt gcgaaatcga ccgttgccgc cgttatcgaa tacctcggtt
    20101 tacaggaaac ggtaaatcag gcttctggcg cattacagaa aaaccagaac ggcgcagata
    20161 ttccggggaa agataccttc accaaaaata ttggtgcctg ccgcgcatat agctcatggc
    20221 tgaatattgg tggcgatagt caggtctgga caaccgcgca atttatttcg tggctggaga
    20281 gtcagggagc atttaaccat ccttactgga tgtgcaaagg ctcatgggct tatgcaaata
    20341 ataaggtcat tacagataca ggttgcggaa atatttgtct tgcaggtgct gtggtggaag
    20401 ttattggcac tcgcggcgca atgaccatac gcgttaccac gccgagcacg tccagcggtg
    20461 gcggaattac taacgctcaa ttcacttata ttaatcatgg tgatgcttac gctcctggct
    20521 ggcgacgaga ctacaacacg aaaaatcaac aacctgcatt tgctttaggg caaacaggaa
    20581 gcactgtcgg aaatgataaa gctgttggct ggaactggaa tagcggggtc tataacgcaa
    20641 acattggtgg cgcatcgaca ttaatcctcc acttcaatat gaatacgggg agctgccctg
    20701 ctgtacagtt ccgcgtgaat tacaggaacg gcggtatttt ttatcgttca gcgcgtgatg
    20761 gttatggctt tgaagctgac tggtcagagt tttacaccac aacccgcaaa ccctctgcgg
    20821 gagatgttgg tgcatatacg caggcagaat gtaactcaag gtttattaca ggtattcgcc
    20881 ttggtggtct gttatctgtt cagacatgga atggtcccgg atggtctgac aggtcaggtt
    20941 atgttgttac cggttccgtt aactcaaaca gagatgaact aattgataca actcaggcaa
    21001 ggccaattca gtattgcatt aatgggacgt ggtataacgc ggggagtatt taacgatgat
    21061 gcacttaaaa aacatttctg ctggtaatcc tgaaacaaaa gagcaatacc agctaacgaa
    21121 acaatttaac atcaaatggc tttatacaga ggatgggaaa aactggtatg aggaacaaaa
    21181 gaactttcag cctgatacgt tgaaaatggt ctatgaccac aacggcgtta ttatttgtat
    21241 tgaaaaggat gtttcagcaa ttaatccgga aggcgcaagc gtcgttgagg ttcctgatat
    21301 tacagcaaat cgccgggctg atatttcagg taaatggatg ttcaaagatg gcgtagtgat
    21361 aaagcgaact tataccgggg aagagcagag gcaacaagcg gaaaatgaaa agcaaagcct
    21421 gctacagctc gtcagggata aaacccagct atgggactca cagctacggc tgggcattat
    21481 ttccgacgag aataaacaaa aattaaccga gtggatgctc tatgcgcaga aggtcgaatc
    21541 cacagacacc tccagcctgc cagtaacgtt tcccgaacaa ccagaatgaa acaaggcccg
    21601 atatcgggcc ttaattttta ctcaggcttt tgtggccatt ctggctttgc cgtatccaca
    21661 cggctgacca gaacactgta gcgttcccat gcttccagtc gtgtgcgttc ctcgtccgtc
    21721 gccatattca gcctgacagc gcgttccagc ggctggatga ctgattcagc ttcggaaagc
    21781 aatgcggcct tttgtgattc ggcctgttgt tgctgttcgt cagccgtata aatccgttta
    21841 accacagctc cgtccttaaa catccactta ccggaatcat cagcacgacg attagctgta
    21901 atatcaggaa cctcaacgac gctataacct tcagggttaa gcgtggaggc atctttggtg
    21961 atggcgacaa taatattatt tgcatcgtaa acaatcttta ttgtgtctgg ctgaaagttc
    22021 ttcacttcct cataccagtt ttttccgtcc tcagagtaaa gccagatgac tccgtgcttc
    22081 tttgttaact catactgctc cactgtttta gcattacctg cttttatgtt ctttaagtgc
    22141 atcatattaa atactcgcca cattatacca agtgccattt atatactttt gcactggcct
    22201 gtaatgaacg cctgcaatat tatcggcaga gttttttccg gtatcctgta cgttaatccc
    22261 cgtcagtaca tagcctgacg ggcactgaaa attccatgtt tgccagttgt tcactccata
    22321 atattgctgt gaaccaagtc gaacatcttt cacatatctg gaatcaaaat tgccatagtt
    22381 gccgggaata acttgcgagc cgcaaagcca gttcccgtta ttatccatgt acgcctgacc
    22441 atcggtgcca ttggctgtcc ttgagttatt aatcatgtag atgccaaatt gcttatttcc
    22501 cagaccgcca atcataaatt tgcggtcggc atggtcctga cggagcaaag cctgagcacc
    22561 atcagtggat accgcattac gtcccaaaat aacattctgg tcacgcatat gaatccacat
    22621 gccggtacta ctgttaattg caaaacggtt tgcaaatata tcccctgtaa catcaagacc
    22681 atgccccatg cttatccggc cagttctgag attaagcgta aaggggcgta gtggccctat
    22741 attaccattt tccccttcat tctctcgtgt agggatgata tgcaggcatt cttcagaacg
    22801 acgaaaaatg gcaccaaaag atgaattaaa tatcctcagt gcattgactg tcgatatttt
    22861 cacttcactg ctgaaaaggg cttcaacaag aacagacaaa gcattccatt taagattcat
    22921 caggtcttta ggccgggtgc caatgatgcg gtgtctccat ttgaaatatt cattgccgtt
    22981 gttgcctgtt tcaaaccaca tgtaggaatc ggtgtctgca tccgaattat ttttaaaccc
    23041 aatctttgcc cagtcagtat ttcgaatcca ggcaaggatt gagtcgtttt caaaagtaag
    23101 tccaccggac aacgtatcgc catttttttg cacggcgtta ccagccttgt ttaccgtttc
    23161 ctgtaaaccg aggttttaga taatggccgt ttccggcctg catggcatga tttgcgcttt
    23221 tggacgggag attcagcgtg ctgattggct atgtaagggt atcaacaaat gaccagaata
    23281 cagacctgca acgaaacgct cttgtttgtg caggatgtga acaaatattt gaagataaat
    23341 taagcgggac aaagacagac cgaccgggat taaaacgcgc tttaaagcgc cttcaaaaag
    23401 gtgacacgct ggttgtctgg aaactggatc gcctcgggcg aagcatgaaa catctgattt
    23461 ctctcgtcgg ggaactacgg gagcgaggaa ttaattttcg cagtctgacc gacagcatag
    23521 atacatcttc tccaatgggg cgttttttct tccacgtgat gggtgccctg gctgaaatgg
    23581 aacgtgaatt aattgttgaa cgtacactgg ccggactggc ggcagcacgc gcacgggggc
    23641 gcacaggcgg acgtcgaccg aagctgacaa aagaacagca tgagcaaata gcaaggctga
    23701 tcaaaaacgg gcacgacaga aaacaactgg caataattta cggcattggt atatcgacga
    23761 tttatcgtta ccaccccgca ggagaatcaa tcggaacaat agagaagagt caggaaacaa
    23821 aataaccgct aatctgacca ttagcggttt ttgtgttaaa tcagaacaac cctttaactg
    23881 aactggccgc gctgttaagg gatgatgtta ccttatcttt gaagccggac agcatatcgc
    23941 tgaacgatga ggattgcagg cgctcccgca aatcctcatc acagcgttca agagtcagtg
    24001 aaaattctat ctttttcgcc ttaccgtagc gatcaaactc ggagcgggtc gtattcgttt
    24061 cggtcaggac atacatgccg taaatctgcc cgacgccatc aatcagaggc cagggtcgtc
    24121 ctgtatacgc ctgcgtggtc agcagcgaca gcgacacttc gccacctgta atttcaggat
    24181 aaagcacacc agaaagaacg atgcgctcat cacctgcacc gatatactgc cagcttgccg
    24241 aacggttaac gcgttcattt ttcacatgcc gccagctttt gttttgctgt aactgctgat
    24301 gcggcaatgt acgcagctca aaaacaaaca tgccgtagat catcatcatg gccatgactc
    24361 ctcaatcttt atcgtaaaaa ctgccacgcc cggcacgggc gcgccgttcc atttctgccc
    24421 tgaccatttc accgaccagt ttcgccagtt cgcggggatt ctgcgtaaca acgttatgca
    24481 gatgaacatg aatttcacca ccaaatccgg aggcaacagg ctcccggtta cgggaagtta
    24541 caggaactga tgccactgga gagcgtatgg cctccgccac cgggcgggag ctggccgcaa
    24601 caacagggac cagcgccgga ggcagcggag ccgggaccac gggtgtgata ttaattgcgg
    24661 gggcaggctt actgacctgc gcaatcttcc gctcctgcca ctccccacga acagcaagtg
    24721 cgcggggcag gttcttaaag acaatatcgc cggggccaat gcgttttttc gtctcatcaa
    24781 ccagcttacc tgtgttatca gcaattttgc tgagtctgcg tagcgtcccg gtattgctgt
    24841 ctgtgagcgg tttgttgtct ttgggtttat cacctccggt gccattgcca ttttccacag
    24901 gcttcggcgg attgattttc gccaggtccc cctgaagcaa ggcaaccttg tcctgaagaa
    24961 tggccgcacg ctgtgcgtct tcgattttct tgcgcgccct ttccgcttca tccggaagga
    25021 cgccaagttt ttcaagtatc cacgccagcg tatccagtag catttttgca ggtgtcagaa
    25081 caagttgtaa cgcaccgcca agaacgttac cgaatatctc gccagcactg gcacatttat
    25141 ccagcgtttc cttgctggac tccatcggtg acagcagcga tttaaaccag ttaaagacct
    25201 gaataatccc atcgcgcacg acatcaaaaa cagggccaaa ccgttcaaag gtttcgcgca
    25261 acggggtcag cctttccata atcccgctga acaccccggc aaaaaatgcc ctgatgggat
    25321 cccagtattt ccagataaga acggcagctc cggcaagcgc agccacgata agactaaccg
    25381 gactgaacag cgccccgata gcgccaccaa gtaaagaaac ggaacccgtc accattcccc
    25441 ataatgcagg cagaaccctg acaacattca ttgattcggt caggagagaa aaaccgagac
    25501 gcaacgtagc cagcttcccg tgaagcaccc caataaccag agacaacgaa ccaaccgttg
    25561 ctgttgtcgc cagcagtgcg cctcccgcta tcagtaactg gcgcgtcagt gccgggtggg
    25621 cctgcgccag cgccgtcacc tttgagacaa cccgcgtgag ccactgcgtg acagaacgca
    25681 gcggaccgtc aaccagatca ctgatgcgaa tacgcagacc ttcccatgca ctgccgagtg
    25741 atttcaggtt gccatcaagg ttattggcca taacctttgc cgtgcgttct gcctcaccgc
    25801 gcgcgccttc aagttctttt ctcagtttgg ctaagaagcc gtcacctgcc gcatcaacga
    25861 ggcccataaa tgatgtgaaa gcctcttctc cggcaatgtc cttaaagaac gatacccggt
    25921 caacctcccc gtatttgcgg gtggctttat agagatcggc cagcacattc tccatcgggc
    25981 gcattttgcc tttggcgtca gcaactgaca cccccagctc tttcagtgct tctgccgctg
    26041 cctttggcgg tgatgacaga ttggccaggc ttttacgcaa cgctgtacct gcatcactcc
    26101 ccctgatacc catcttcgcc agcacgcccg ccatcgctgc ggcctgctcc agcgatattc
    26161 ccagcttacc cgccaccgga cctgcatatt tcatggtttc gcccagtgcg cgaaggtcag
    26221 tgttggtacg ggtaaacgct gcggtaagtg tgtcaccgac ccggtccatc tggtcagcag
    26281 agaggccgaa ctgcgtcagg atattggaac caatatccgc cgtctcgccg aggtccatac
    26341 cgccagccgt tgccatgctc agcacgccgg gaagcgcagc ctgaatggcc tgcggtgtga
    26401 aaccagccat tgcaagaaat gcctgtccac tggcggcatc gcctgcggtg aactgcgttt
    26461 cagagccaag ttttaacgcc tgctcacgca gcgccttaaa ctgcgggctg tttttatcga
    26521 ttcgcgtcag tgcctgaaca cgggacatct ctttcccgaa cccgatagcg ggctgcaaaa
    26581 aacgcccggc agcatagccg cccgctgccg ccgcacctgt tgccagcgca ccacctgttt
    26641 tcagttttcc cgctgtttcc tgcgcgcgtg aataccgctc acgcgcctgc gttacacgcg
    26701 caagcgcccg ccgttcgcgt tcaagctggt tgttgtattg tccggtgcgt ctgatggcct
    26761 gctggatggt gttatcgctg cctgtcaggg aaatgccgtg gcgtttcagc tctccgccaa
    26821 gttcccgcat tttctgaatt tcccgtgtgc gcgattcatt caggcgttca agccgggtgc
    26881 ttaactgctg catcagcttt tgttgttttt cgctgagcac tgtacccgtg cgttgtaact
    26941 gattaagggc gttaagctgg cgtcgtgctt tcacgatgcc cgcatccgct ttactgacag
    27001 cgtcacgggc gcgctcaaat gaacgcgcct gacgctcgag atttttgatc gccccctgcg
    27061 ttcgctggat ggagtcacca aactgcccca tcaggcggcg ggcgttttcg gcaggccggg
    27121 tcagcctgtc aacggcgctg aaagcgaccc ggatatcaag agtcttcatt gtctgcattc
    27181 ccgctgcgaa gtgccgcccg ctcacgccag ctaaccactt cgccgggcgt catcatgaag
    27241 atttcggcgg gcgaccagtt aaaaatggcg gcaatatccg ccacaaagtc ttctatgtgc
    27301 tcaaagcaca caaccgtgat caggcttccg tcgcctgttc gttcttctcg ccagagtccg
    27361 caccgctcaa aaaatttacg gcaaccacac ataactgaat aaagtcacgg gatgccattt
    27421 ttttgatcgt cacttcatcc agtcgcggtg atgtcacgcg tgacagcagc gtaaacatgg
    27481 attccgcttt caaattcagc acatcagaca gcgacaaatc tcgcagagat ccagcctgct
    27541 caatagcccc ggtgatctcc acatacgtga ttttttcgcc gcctcgctca attggttggg
    27601 taagttttac gccacgctca ctggtttctt tcacagtgtc agcaacgacc gtgttttcgg
    27661 tatcgatgtt tttcgtctct ttcatcagga aactcctttc agtcagaggc gacgcactgc
    27721 gccgcctgca tattacttat cagccaagcc cgagcgcgga acggatgcga tcgggcacaa
    27781 tgtccctgcc gtccttccgg taaatgaagt tcagcaggtc aatctcccac aacgggcgat
    27841 cgttaacact cagcttgtag taggtgtttt taatggcgta agtgtgtgat gtggcttcgc
    27901 cctgtttggc ttcccccata tcaatttccg tcacacgtcc gcgcatttcg acttcataca
    27961 ggtcgctttc agcgtcggtg tagtattcac ccgcaaaacg cagcagcgta ccgtcaatcg
    28021 tgccgccata cttaaggaac agctcacgaa ctgcgccccc catgacaaag ctcgcatcaa
    28081 gcgcggagtc atccaggccg agatcaatac ttaccgctcc catcatgcca ccaccccggt
    28141 agctgtcggt tttgcgcgtc agcttaggca gagtgacgga cgtcacctta cccacttcgt
    28201 tttcaccatc cacaaacagc gtaaaaaagc gaagatgttt tggtacagcc atcaggcacc
    28261 tcccagcacc gcaaatgcgg gaccaaagaa ttcatcagta aacgactggt aaagctccat
    28321 gtcttccagc gggggaacag gcgtatattt gtagcgaata cgcacgcgcc cctgacgtaa
    28381 attcgtggtg ccgttatcca ccacgtcata ccagcacgac gccccaatca gtttcccggc
    28441 agtaaccagt gaatccagtt ttgccctgat ggcaccgata acatccttca cgttcgcagg
    28501 cgtcagtgga ctgtcgatgg tttcaaactg cgcttccgca attgaatcag ccagcacctg
    28561 tgcggttcgg gtatacacct caaagatgta ggcgttcgtt tccggtgtgc ggttgcccca
    28621 gaagcggaac ccgttgcgac gaataatggt cgtgatttct ttgttgttga ggctgttggc
    28681 atcactgtct tcggcctgca acgaccagaa cacatgcctg gacatcccca gcacattttt
    28741 aaccggaacg ttggacagcg atttgtgcca gccctgctca tggtcaatgt acgcacgaag
    28801 gccgcacgca taggcaggcg cggggaacgt ttcgtttttg ccacttttcg ggttgtaggc
    28861 gatgaagtcc ggccataaga gcatcacctc acgttcgttg aatttctggc ggtaggtaat
    28921 tgcctcagcc atcgtgttac agtcatgaca tgaggcatac acaaacgcgc gcagtttacc
    28981 cgcaatcacg cacagggatt ttgtcaccgc ctccgtgtcc agctccggcg cggccagaat
    29041 acgcggacgg tatccgatgc tttcatcctg ctctgcaaca agcagcgcat acatccccgt
    29101 atagctgccg tcagattcag aaccaccgat aaccagttga tcctgcgttt ttccgtcttc
    29161 ttctttgtgt tcagccacgc gaacgacgat cacctttgtg ctcacctggt ctgcgatggc
    29221 cttaagcgca cgataaagcg tccccgttgt tccgcatttt cccagcacgt cattgacgcg
    29281 gggcagcagt gtgggcttgt tcagcgggaa cagctccgcg tccgcatcat ccgccgttgc
    29341 cacgataccg ataacgctgg aatcaacatc gttaatcgct gttaccaggt cggtattttc
    29401 cgtaacacgg gcaccatgaa aacgagtttc actcatagct tcaacccctt gtatccgtta
    29461 aatgattcgg caacaatcat cacccaccac gcgcgtaatc tcacccctgc gccattctcc
    29521 cgccacggcg acaacaaaaa gcagtaaccc cctccgcacg cacatgcgac catgccacac
    29581 agggagggag cagatgaccg acaccaccat gcaattgctc agtcagggca cagaccccgt
    29641 gaaaatgccg gattttgata ttctcgcgga gggtaaaacg ctgtcaggcg tggcagagcg
    29701 cctgatgagc ctgtcactga ccgacaaccg gggatttgag gcggaccagc tcaccatcac
    29761 gctggatgat gcggatggtc agttgcagct accgccacgg ggcgcgcgcc tgacggttct
    29821 cattggctgg aagggagaac cgctgacaga aaaaggcact tacattgttg atgaaatcgc
    29881 tcacgaagga ccgccggaca ggctgactgt ttcagccaga agcgcagatt ttcgggctga
    29941 atttaacgtt aaacgtgagg tgtcctggca tgatgtgacc gttgagcgtg tggtatccgc
    30001 catcgctcat cggtatggtc tgaaaccgca aatcagcgaa atgctgatgg atatcgaaat
    30061 cgaccacgcc gaccagaccg aagaaagcga catgtccttc cttacgcgca tggcggaaat
    30121 gctgggcgca atcaccacgg taaaaagcgg taatctgtta ttcatcatgc ccggtggtgg
    30181 cgtgaacgca cagggccagc cgttgccctc gttcgccatc acacgcagca gcggcgatcg
    30241 ccatcagttc cgcattgctg accgcgaagc gtatacgggg gtacgcgctt actggcttga
    30301 tcttaattac gggaaaaaga aaaaagtcag cgtgaaacgc cgcaaaccgt caaaaccaaa
    30361 aaaggagaaa agcagcagcc gggaaggcga ctatatggaa ggtgcggaag gcaacgtgtt
    30421 tgtgttacgc aaaacttatc agaacgagca ggcagcaaga cgtgcagcgg cggcaaagtg
    30481 gcagcagcta caacgcggag ccgcatcatt ctccatcaca ctggcgcatg gacgtgcaga
    30541 actctacccc gaaatgcatg gcacggtaac aggatttaaa agcgagattg ataatcagga
    30601 ctggattatt gcaaaagccg agcacaccat tgataacagc ggctttacca cgcagcttga
    30661 gcttgaggca aaaatcccgg aatggatagc agaaacagag tgagcaactt aaatgtatta
    30721 actcagaacg gatcaacaca ctcacaacat agagtcaata tagctgctcg ctttgttcca
    30781 gcaaggatgc ctatttctaa ggaaaaacag agcagaaaca tatggtgttt tcctacctga
    30841 catcaggggt g
//
LOCUS       DAESHI010000043        30154 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.043, whole genome shotgun sequence.
ACCESSION   DAESHI010000043 DAESHI010000000
VERSION     DAESHI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30154
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.043"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="KW011_002014"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="KW011_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBH7660347.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="KW011_002015"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="KW011_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBH7660348.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="KW011_002016"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="KW011_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBH7660349.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="KW011_002017"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="KW011_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBH7660350.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="KW011_002018"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="KW011_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBH7660351.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="KW011_002019"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="KW011_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBH7660352.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="KW011_002020"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="KW011_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBH7660353.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="KW011_002021"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="KW011_002021"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBH7660354.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="KW011_002022"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="KW011_002022"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBH7660355.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12918..14051)
                     /gene="thiH"
                     /locus_tag="KW011_002023"
     CDS             complement(12918..14051)
                     /gene="thiH"
                     /locus_tag="KW011_002023"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBH7660356.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="KW011_002024"
     CDS             complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="KW011_002024"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBH7660357.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="KW011_002025"
     CDS             complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="KW011_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBH7660358.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="KW011_002026"
     CDS             complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="KW011_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HBH7660359.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="KW011_002027"
     CDS             complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="KW011_002027"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBH7660360.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="KW011_002028"
     CDS             complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="KW011_002028"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBH7660361.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18363..18463)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18516..18992)
                     /locus_tag="KW011_002029"
     CDS             complement(18516..18992)
                     /locus_tag="KW011_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rsd/AlgQ family anti-sigma factor"
                     /protein_id="HBH7660362.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKLGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19087..19860
                     /gene="nudC"
                     /locus_tag="KW011_002030"
     CDS             19087..19860
                     /gene="nudC"
                     /locus_tag="KW011_002030"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HBH7660363.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19900..20964
                     /gene="hemE"
                     /locus_tag="KW011_002031"
     CDS             19900..20964
                     /gene="hemE"
                     /locus_tag="KW011_002031"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HBH7660364.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYSLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20974..21645
                     /gene="nfi"
                     /locus_tag="KW011_002032"
     CDS             20974..21645
                     /gene="nfi"
                     /locus_tag="KW011_002032"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HBH7660365.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21688..22278
                     /locus_tag="KW011_002033"
     CDS             21688..22278
                     /locus_tag="KW011_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HBH7660366.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22465..22737
                     /gene="hupA"
                     /locus_tag="KW011_002034"
     CDS             22465..22737
                     /gene="hupA"
                     /locus_tag="KW011_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HBH7660367.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22750..23445
                     /locus_tag="KW011_002035"
     CDS             22750..23445
                     /locus_tag="KW011_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HBH7660368.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23447..23872)
                     /gene="zraP"
                     /locus_tag="KW011_002036"
     CDS             complement(23447..23872)
                     /gene="zraP"
                     /locus_tag="KW011_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HBH7660369.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24110..25486
                     /gene="zraS"
                     /locus_tag="KW011_002037"
     CDS             24110..25486
                     /gene="zraS"
                     /locus_tag="KW011_002037"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HBH7660370.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            25483..26808
                     /gene="zraR"
                     /locus_tag="KW011_002038"
     CDS             25483..26808
                     /gene="zraR"
                     /locus_tag="KW011_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HBH7660371.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26805..28094)
                     /gene="purD"
                     /locus_tag="KW011_002039"
     CDS             complement(26805..28094)
                     /gene="purD"
                     /locus_tag="KW011_002039"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBH7660372.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28106..29695)
                     /gene="purH"
                     /locus_tag="KW011_002040"
     CDS             complement(28106..29695)
                     /gene="purH"
                     /locus_tag="KW011_002040"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBH7660373.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7057 a         7866 c         8307 g         6924 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctacgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcactt ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagactcc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacgc cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac atataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgct tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggtaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaat ccctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttggtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggagctg gtgccggaac gcctgcgtgg tgaaactgca
     5101 tctttcgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ttggctaagc tgagccagtc tggtcacaag
     5341 cgtattgaaa cgctgttcac caacgacctg gatcacggcc cgtatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacctgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ctgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggac
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc cgggtggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcatt acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctt tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaggtga ccgacggtgt tgtaactgac gaaattcact acctttctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgtga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtttg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgaccgacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtgtatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccactggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gttctgggtc tgtactatat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcaa acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gagggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg taaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaaca ccgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtgccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 ccagaccatt actcgtcaga ccgacgaact gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga tcccaggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gctcgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtga ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatgggtg ttattcagag ggcaaattct tcacggaaaa tattaagaaa ggcagggcga
    12781 aacgaggaag aagcttttcg ccctggacaa cattcctgct gacggcacta ccataaaaaa
    12841 acacaacgct ttcaacaacc ttgcgttttt catcttccgt agcggctcgg aataaattta
    12901 cgttcttaca ttccgtctca tagtctttgc gaggcgcgtc ccagatagct gtcccagtct
    12961 ttccataccg gctgcaaacc ctgagcggtt aacgcggcag caaccgcttc cggtctgcga
    13021 tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat cggcatagcc acctggctgc
    13081 gttttcgaga aggcgctgac gttattgatc gccagcggaa taacgcgatc gcgaaaccac
    13141 ggtgattccc gcgtggagag tgacagttca atctccggtg caagcagtcg gaaggcgcag
    13201 atggtttgca ctaactggcg ttcatccata atcgacgcag gctcaatgcc gccagtacac
    13261 gggcgcaggc gcggaaagga gacagagtaa cggctttgcc agtaatgctg ttgcagccat
    13321 agcaaatgtt cggcaaccat atagcagtca acgcgccagt tgtcggaaag gccaattagc
    13381 gcgccgaggc ctatcttatc aatccccgca cgccccagcc gatccggcgt ttccagccgc
    13441 cagaagaagt cctgtttttt gcctttcaaa tgatggcggg tataagtcgc ctcgtgatat
    13501 gtctcctgat aaaccatcac cccatccaga ccaagttgct ttaactcggc gtattccgtc
    13561 tccgccagcg gttgcacttc catttgtagt gaagagaact gttcacgcag ggcggggaga
    13621 tgacgacgaa agtaatccat ccccactttc gcctgatgtt caccagtgac taatagcaga
    13681 tgttcaaagc ccatctcccg tatagcggcg ctttccctgg caatatccgc ttcatccagc
    13741 gttttgcgct tgatgcgatt actcatggaa aatccgcagt acgtgcagtc gttagcgcaa
    13801 agattggaaa gataaagcgg gacgtagaaa ctaactgtgt tgccaaatcg ctgacgggtc
    13861 agacgctgcg cccgttgggc cagtggttcc agatagccac tggcggctgg cgataacagc
    13921 gccatcatat cgtcgcgggt gagttgcgag gcatttagcg cccgttctac gtcagcagcc
    13981 gttttgccgt tgatacgcag gcggatgtcg tcccagtcca gttgtcgcca gcgatcgctg
    14041 aaggttttca tgccgatgcc tccagaaatc cagtcagcgg gctggtggca tgagcaaaat
    14101 gactgcggct gcccggtccg gactgacgtg ccagcaggcc tgcttctacc gccagacgaa
    14161 atgccttcgc catgttgacg ggatcgtccg ctacggcaat cgccgtattc actaacaccg
    14221 cgtcggcccc catttccagg gcctgcgcgg catggctggg aacgccgatg ccagcatcga
    14281 caaccaccgg aactgtggcc tgctggataa taatctccag catggcacgg gtttccagtc
    14341 cctgattcga gccaatcggc gcgccgagcg gcatcaccgc tgcacagccg acttcttcca
    14401 gacgtttaca caataccgga tcggccccgc agtaaggcag cacgacaaat ccctgttgta
    14461 ccagcatttc ggcggctttc agagtttcga tgggatcggg caacagccag cgggcgtcag
    14521 ggtgaatctc taattttaac cagtttgtgc ctaacgcttc acgtgccaga tgggcggcga
    14581 aaatggcttc ttccgctgtt ttcgccccgg aagtatttgg cagcagggtc acacccgccg
    14641 cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa gtcgacacgt ttcatcgcca
    14701 gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac catcagttgt gaagaagcga
    14761 atttccctgt gccggtaaac agatgtgaat caaacgtttt gtccgcaata cgtaacatct
    14821 caaccccctg caataacctg aaaaagcagg atctggtcgc catcctgcac gatatgttgc
    14881 gtccactgct cacgcgggac gatttgctga ttaatcgcca gagccgcgcc cgcttgtcgt
    14941 tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg cggcgcactg catcggttga
    15001 tcgttaaaca ggatctgcat tgcttcctcc gcataccggg caaccactgg cgcggcgcaa
    15061 cgccaggctg cgccactggc tcgatttacc gtcgaacagt cggagttctc ccgcaggtgt
    15121 ctctataccg cttaataact taatggcttc cagtgcctgc aaagtgccca taaccccgac
    15181 caccgggcca accacgcccg ccgtgcggca gttgcgttct ggctcctggt tatctggcca
    15241 caggcagcgg taacacccct gctcccaggg cggcgtcagt accatcaact gaccgccaaa
    15301 tccgaccgcg ctggcggtga taagcggcgt gttgagtacc acgcaggcgg cattaatctc
    15361 ctggcgagtc gccatattgt cggtacagtc gagcaccaca tcggcccgtg caaccgcatc
    15421 ttttaacgcc tcacccgtta accgttgttg taatgctgtc agttgaatat cgggattcaa
    15481 ctgtgtcagt cgctgatggc tgacctgcga tttcgggcga tcgatatctt cagtggtaaa
    15541 gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg tcatctgcca gtaccagcgt
    15601 cccgacgcca gcccccgcca ggtatagcgc agcaggtgta cccagcccgc caagaccaat
    15661 aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc agagcgatat cgtcgagcag
    15721 aatttggcgg ctataacgca taaagtcacg gtcattcatc gccaactcct gcaatttcca
    15781 gcaactgtgc cgttgccaaa cgccagtctg cggcttgagt aatggcgctg acgacggcga
    15841 tactgccgac acccgttgct atcaccgcag gcgcgcgcgc cagactgata ccgccaatcg
    15901 ccacggtggg ataatccgcc agtcgctcaa catgccgtgc cagctgttcc agcccctgcg
    15961 gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg tcccagcgcg atataagagg
    16021 ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg tgtcgaaacg cccagccgca
    16081 ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa atcttcctgc cccaaatgga
    16141 cgccatacgc ctgatgcttg atcgccagcc gccagtaatc gttgataaac aatcgcgcgt
    16201 tatagcggcg gcccagcgca attgccgcca cgacatcggc ttccacctct tcattgcgcc
    16261 gatctttgat gcgtagctgg agagtacgta cgcctgcatc caacagacgt tcgatccact
    16321 gtacgctgtc caccaccggg tacagtccta aacgaaaagg tacaggagga aaatcaggct
    16381 gatacatcac gcttcctcct tacgcaggta gatttctccg cctctggcgc ggaagttctc
    16441 cgacatatcc gccattccca cttcaatggt ttgcgcggcg gcgtaatcac gtacttcctg
    16501 gctgattttc atcgagcaga atttcggccc acacatggag caaaaatggg cgactttacc
    16561 tgactcttgc ggcagggttt catcgtgata agcgcgggcg gtaaacgggt cgagggccag
    16621 attaaactgg tcttcccagc gaaattcgaa gcgggctttc gacatggcgt tatcgcgaat
    16681 ttgcgcgcct ggatgccctt tcgccaggtc agcggcgtgg gcggcaatct tataggtgat
    16741 aagcccctgc ttaacatctt ctttattagg cagacccaga tgctcttttg gcgttacgta
    16801 acagagcatc gcgcagccaa accagccaat catcgccgca ccaatccccg acgtgaagtg
    16861 gtcatagccc ggcgcaatat cggtagttag cggccccaga gtgtaaaacg gcgcttcgtg
    16921 gcagtgctct aactcctcgg tcatattgcg gcggatcatc tgcatcggca cgtggcctgg
    16981 gccttcaatc atcacctgca cgtcatattc ccaggcaatt ttggtcagtt cgcccagcgt
    17041 atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga atagaaccgg ggcgcagacc
    17101 gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt tcacaaattt cgcggaagtg
    17161 ttgatagagg aaattttcct gatgatggga gaggcaccat ttcgccataa tcgaaccgcc
    17221 gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc acatagcgca gcagtacgcc
    17281 cgcatggata gtgaagtaat ccacaccttg ctcggcctgt tccagcagcg tgtcgcggaa
    17341 cgcttcccag gtaagatctt cggcgatccc gttaaccttc tccagcgcct ggtagatcgg
    17401 cactgtaccg atcggcaccg ggctgttacg caaaatccac tcgcgggttt cgtgaatata
    17461 gcgaccggtg gagagatcca tcaccgtatc cgctccccag cgcgtggacc ataccagctt
    17521 ttccacttct tcttcgatgg aagaggtgac cgccgagttg ccgatattgg cgttaacttt
    17581 taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc ggatgattaa tgttggccgg
    17641 gataatcgca cgtccggcag caacttcatc acggacaaat tccgcagtga tattttccgg
    17701 cagatgtgcg ccaaagctca ttcccggatg ctggtggcgt aaaacctcgc tacggatgcg
    17761 ctcgcggccc atattctcgc ggatggcgat gaattccatt tccggcgtga tgatgccctg
    17821 gcgggcgtag tgcagttggg tgacacggcg tcctgttttg gcgcgttttg gcgttaataa
    17881 gccgctaaaa cgtagctcgt cgagaccatc atctgccagc cgcgttctgg tgtaatcgga
    17941 actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg atccacggct ggcgtagttt
    18001 tgccagccct tgctgcacgt taatggcgat ctgcggatca ccatacgggc cggaggtgtc
    18061 gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt tctttgctac cgccaattag
    18121 cgtcgggcta agctggatct cacgcatcgg cacgcgcacg ccggggtgtg tgccagtgat
    18181 ataaatgcgt tttgagttgg gaaaggcggt gccttccagg gtgtcgataa aatgttgggc
    18241 ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac atagctcatt ccaaaaagtc
    18301 aaggacgtgg cttgtcagac gacggatgaa gcaagagacg atcgccgcaa aggcgatgcg
    18361 atagcagatt aactcttgtt cccttcgcag gtattagcct gatcaggttc cgcggatccc
    18421 gaataaacgg tctcagccag ttaaggcact ccgacaagaa attagccccc acaaacgggg
    18481 cgttgaatgt aaattacgcg ttaacagcgc agaactcaag caggatgttt gacgcgggcg
    18541 gcgtcaagca ccagcagaat cagcttatct tccagcacaa agcgcgcttc cagcgcctcg
    18601 ccgatgtcag ataaaacctg ttgaaactca aggtaattat catgatcgat ggcggtttcc
    18661 aggctggaat cgtagtaatc cataatctgt tgggtattgg cttcgagttg cggccaaatc
    18721 ttcgcggcgc gcgcgagttg cccgttgcct tccagcttat gaagaatgcg ctcataaata
    18781 ctgaaatgtc cggcagacaa gtaatcgacc aggctctgac aaaaatcatc aagggctttt
    18841 tcgtttagcc tcatgtacga ttctttgcca agcttaatgc caaccagatt gtagtaagcc
    18901 acgagcagat gcttacgtac atgtagccag cgatcaacca gtttgttact tcctctgacg
    18961 cgctccgtca ggttatcgag ctggttaagc atgattgact ccgcaagttt gtattcaaaa
    19021 actgctcagt gagaaatgta aaaaccatgt taaacatgcc agtgatgcaa aggtagtgca
    19081 agagctatgg atcgtataat tgaaaaatta gatcacggct ggtgggtcgt cagccatgaa
    19141 caaaaattat ggttgccgaa gggagaattg ccatatggcg aagcggcaaa tttcgatctt
    19201 gtgggtcagc gcgcactaca aatcggcgaa tggcaggggg aacctgtttg gttagtacaa
    19261 cagcagcggc gtcacgatat ggggtcggta cgtcaggtca ttgatctcga tgttgggctg
    19321 tttcaactgg ccggacgagg cgtacaactg gcggagtttt accgatcgca taaatactgt
    19381 ggttactgcg ggcatgaaat gtatccgagc aaaaccgaat gggcgatgct gtgcagccat
    19441 tgccgtgagc gttactaccc gcaaatcgcc ccctgcatta ttgttgccat ccgtcgcgat
    19501 gattcgatcc tcctcgccca gcatacccgc catcgtaacg gtgtccatac agtacttgcc
    19561 ggattcgtcg aagtgggcga aaccctcgag caggcagtcg cgcgggaagt gatggaagag
    19621 agcggaatta aagttaaaaa cttgcgttac gtgacttctc agccgtggcc gtttcctcag
    19681 tctttaatga ccgcgtttat ggcggaatat gacagcggcg acatcgtgat cgacccgaaa
    19741 gaattgctcg aggcgaactg gtatcgctat gacgatttgc cgttactccc gccgcccggc
    19801 accgtagcgc gccgtctgat agaagatact gtggcgatgt gtcgggcaga gtatgagtga
    19861 tgatacactg accgcctgac gcactaagga acagccaaaa tgaccgaact taaaaacgat
    19921 cgttatctgc gggcgctgct gcgccagccc gttgatgtca ctccagtatg gatgatgcgc
    19981 caggcgggtc gctatctacc ggaatataaa gccacgcgcg cccaggcggg cgattttatg
    20041 tcgctgtgca aaaacgccga gctggcgtgc gaagtgactt tgcaaccgct gcgtcgctac
    20101 tcgctggatg cggcgatcct cttttccgat atcctcaccg tgccggacgc gatggggtta
    20161 gggctctatt ttgaagccgg agaaggtccg cgttttacct cgccagtcac ctgcaaagcc
    20221 gacgtcgata aactgccaat tccggacccg gaagatgagc tgggttacgt gatgaacgcg
    20281 gtgcgtacca ttcgtcgcga actgaaaggc gaagtgccgc tgattggttt ttccggcagc
    20341 ccgtggacgc tggcgaccta catggtggaa ggcggcagca gcaaagcgtt caccgtgatc
    20401 aaaaaaatga tgtatgccga tccgcaggcg ctgcacgctc tactcgataa actggcgaaa
    20461 agcgtcactt tgtatctgaa tgcgcagatt aaagccggtg ctcaggcagt gatgattttc
    20521 gacacctggg gcggtgtgct taccgggcgc gattatcaac agttctcgct ctattacatg
    20581 cataaaattg ttgatggttt actgcgtgaa aacgacggtc gccgcgtgcc agtcacgctg
    20641 tttaccaaag gcggcggaca gtggctggaa gcgatggcag aaaccggttg cgatgcgttg
    20701 ggcctcgact ggacaacgga tatcgccgat gcgcgccgcc gtgtgggcaa taaagtcgcg
    20761 ttgcagggta atatggatcc gtcgatgctg tacgcgccgc ctgcccgcat tgaagaagaa
    20821 gtagcgacta tacttgcagg tttcggtcac ggcgaaggtc atgtctttaa ccttggtcac
    20881 ggcattcatc aggatgtgcc gccagaacat gctggcgtgt tcgtggaggc agtgcatcga
    20941 ctgtctgaac aatatcaccg ctaaggagtg attatggatc tcgcgtcatt acgcgctcaa
    21001 caaatcgaac tggcttcttc tgtgatccgc gaggatcgac tcgataaaga tccaccggat
    21061 ctgatcgccg gagccgatgt cgggtttgag cagggcggag aagtgacgcg agcggcgatg
    21121 gtgctgctga aatatccctc gcttgagctg gtggagtata aagttgcccg catcgccacc
    21181 accatgcctt acattccagg ttttctttcc ttccgcgaat atcctgcgct gctggcagcg
    21241 tgggagatgc tgtcgcaaaa gccggattta gtgtttgtcg atggtcatgg gatctcgcat
    21301 cctcgccgtc ttggcgtcgc cagccatttt ggtttgatgg tggatgtgcc aacgatcggc
    21361 gtggcgaaaa aacggctctg cggtaaattc gaaccgctct ccagcgaacc gggcgcgctg
    21421 gccccactga tggataaagg cgagcagctg gcctgggtct ggcgcagcaa agcgcgctgt
    21481 aacccgttgt ttatcgctac cggccatcgg gtcagcgtgg acagcgcgct ggcgtgggta
    21541 caacgctgca tgaaaggcta tcgtctgccg gagccaacgc gctgggcgga cgcggtggcc
    21601 tcggaacgtc cggcgttcgt gcgctataca gcaaatcagc cctaattcag gtaaactgcg
    21661 gataatttcc gtatttgaga actcaacatg ttacaaaacc caattcatct gcgtctggag
    21721 cgcctggaaa gctggcagca tgtcactttc atggcttgct tatgcgaacg catgtacccc
    21781 aattacgcca tgttctgcca gcaaaccggt tttggtgatg ggcaaattta ccgtcgtatt
    21841 ctcgatctca tctgggaaac gctgaccgtt aaagacgcaa aagtaaattt cgacagccaa
    21901 ctggagaaat ttgaagaagc gattccttca gccgacgatt tcgatctgta cggcgtttat
    21961 ccggcaatcg atgcctgcgt ggcgttaagt gaactggtcc attcgcgttt gagtggtgaa
    22021 acgctcgaac acgcggtgga agtgagtaag acctccatca cgaccgttgc gatgctggaa
    22081 atgactcagg ctggtcgcga aatgagcgat gaagagctta aagaaaaccc agctgtagag
    22141 caagaatggg acattcagtg ggaaatattc cgacttttag ctgagtgcga agaacgcgat
    22201 atcgagctga taaaaggcct tagggcagac ctgcgtgagg cgggtgagag caatattggt
    22261 ataatttttc agcaataaga ccagaaaacg tgatttaacg cctgatttgt cgtacctgga
    22321 gtcttccctt tcgccccccg tctggtctac atttgggggg cgaaaaaaag tggctatcgg
    22381 tgcgtgtatg caggagagtg ctattctggc atttccgtcg cactcgatgc ttagcaagcg
    22441 ataaacacat tgtaaggata acttatgaac aagactcaac tgattgatgt aattgcagag
    22501 aaagcagaac tgtccaaaac ccaggctaaa gctgctctgg agtccactct ggctgcaatt
    22561 actgagtctc tgaaagaagg cgatgctgta caactggttg gtttcggtac cttcaaagtg
    22621 aaccaccgcg ctgagcgtac tggccgcaac ccgcagaccg gtaaagaaat caaaattgcc
    22681 gcagctaacg taccggcatt tgtttctggc aaggcactga aagacgcagt taagtaagat
    22741 tgcgtggcag tgaacagttt taacgaaggg gtggtttcac cccttttgtc tttctggcgt
    22801 cgatcattga tgctggctgg cgctctgctt ctcactgcct gtagtcataa ctcttcactt
    22861 cctcccttta ccgccagtgg atttgctgaa gaccagggcg cggtacgcat ctggcgaaaa
    22921 gacagcggcg ataatgtgca tctgcttgcc gtgtttagcc cgtggcgcag tggcgatacc
    22981 acgacgcgag agtatcgctg gcagggcgat aacctcacgc tcatcaatat caatgtttac
    23041 agcaaaccgc cggtgaatat tcgcgcgcgt tttgacgatc gcggtgatct gagctttatg
    23101 caacgtgaat ccgatgggga aaagcagcag ctttctaacg accaaatcga tttataccgt
    23161 tatcgtgctg atcagatccg ccagattagc gatgccttac gtcaggggag agtcgtgctg
    23221 cgccaggggc gctggcatgc gatggaacag accgtgacca cctgcgaagg gcaaaccatt
    23281 aaacctgatt tagattcgca ggcgatagcg catatcgagc gccgccagag ccgctcttct
    23341 gttgatgtca gcgtggcatg gctggaagcg cccgaaggtt cgcaattact gttagtggca
    23401 aactctgatt tctgtcgctg gcaacccaac gagaaaacgt tctgatttac cagtggccca
    23461 tacccatatg accgccacca ccgcagccgc cgtagcccat gcccattccg gctccgcgcg
    23521 gaatacccgc ttcagccatc gcgatatctc gtttcacccg taactcatct aacgactgac
    23581 gcaaattctc catctctttg gcgaccgcgt taattttgct gctatccggt gggttcgcgg
    23641 ctaacagggc attgtattca taacgcttcg tcaccagttg ctgttgcagt gcgctgcttt
    23701 gagcgtaaaa gtcattatgg attttctgcc acgctgtctg ttgttcgctg gtcaaaggcg
    23761 cggcattttg ctgccacata ccgtgtccgc cgtgagcaaa tgcagatgtc gatcccatcg
    23821 ccattgctga aagcgccatc attaccaggg caattttcgt gttccgtttc atggttaatc
    23881 ctccagtggt tgtcttactt cgggtattgc atcttccgtg ccaacgatga aacgctgata
    23941 tgacgggtaa tctggcatga taaacgagta aaaatgactc gcctgctgcg ggtagcgagt
    24001 catttttact cattgaaact taagcctttg tgttacagcg cagggtaagc gctgataaaa
    24061 gatggcatga tttctgctgt cagaaaggga tgagcaggca aagaagaaga tgcgttttat
    24121 gcaacgttct aaagactcct tagctaaatg gttaagcgcg atcctccccg tggtcattgt
    24181 tgggctggta gggttgtttg cggtgacggt gattcgcgat tatgggcgcg agactgccgc
    24241 cgccagacaa acgctgctgg aaaaaggcag tgtacttatt cgcgctcttg aatccggctc
    24301 gcgcgtcggc atggggatgc gcatgcatca tgcgcagcag caggccttac tggaagaaat
    24361 ggccgggcag cctggagtac gttggtttgc ggtcacggat gaacaaggaa caatcgtgat
    24421 gcatagcaac tccggcatgg tgggaaaaca gctttattcc ccgcaggaaa tgcagcagtt
    24481 acatccggga gatgaagaag cgtggcggcg gatcgatagc gcagacggcg agcctgttct
    24541 ggaaatttat cgccagtttc aaccgatgtt tgctgctgga atgcaccgga tgcgccatat
    24601 gcagcagtat gccgcgacac cacaagcaat tttcatcgct ttcgacgcca gtaatattgt
    24661 gagtgccgaa gatcgtgagc agagaaacac cctgattatc ctcttcgccc tggcgacggt
    24721 cttgctggca agcgtattgt cattcttctg gtatcgccgc tatttgcgct cgcgccagct
    24781 tctacaagat gaaatgaagc gcaaagagaa gctggtggcg ctggggcatc ttgcggcagg
    24841 cgttgcccac gaaatccgta acccactttc ctcgattaaa ggactggcga aatactttgc
    24901 cgagcgcgcg cctgcagggg gagaagcgca tcaactggcg caggtgatgg cgaaagaggc
    24961 cgaccgttta aaccgcgtgg taagcgagct gctggaactg gttaagccaa cgcatctggc
    25021 tttgcaggcg gtggatctca acacgctgat taaccactca ttacagctgg taagccagga
    25081 tgcgaacagc cgggagatcc agttacgctt taccgccaac gacacattac cggaaattca
    25141 ggccgatccg gacaggctga ctcaggtcct gttgaatctc tatctcaatg ctattcaggc
    25201 gattggtcag catggcgtga ttagcgtgac ggccagcgaa agcggcgcgg gcgtgaaaat
    25261 cagcgttacc gacagcggta agggaattgc ggcagatcag cttgatgcca tcttcactcc
    25321 gtacttcacc actaaagccg aaggcaccgg actggggctg gcggtcgtgc ataatattgt
    25381 tgaacaacac ggtggtacaa ttcaggtcgc aagccaggag ggaaaaggcg caacgttcac
    25441 cctctggctt ccggtcacta ttacgcgtaa gggcccacaa ggatgacgca cgataatatc
    25501 gatattctgg tggtggatga tgacattagc cactgcacta ttttgcaggc tttactgcgc
    25561 ggctggggct ataacgtcgc gctggcgaac agcgggcgac aggcgctgga gcaggtgcgg
    25621 gaacaggttt ttgatcttgt gctttgcgat gtgcgaatgg cggagatgga cggcattgcc
    25681 acgctaaaag agatcaaagc gttaaatccg gcgattccgg tgctgattat gaccgcatac
    25741 tccagcgtcg agacggcggt agaagcgttg aaaaccgggg cgctggatta tctcatcaag
    25801 ccgctggatt tcgataacct gcaggcgacg ctggaaaagg cgctcgcgca tacgcacagt
    25861 attgacgctg aaacgcctgc ggtgtctgcc agccagttcg gtatggtcgg taaaagcccg
    25921 gcgatgcaac acctgctcag tgaaatcgcc ctcgtcgcgc catcggaagc cacggtgctg
    25981 atccacggcg attccggcac cggtaaagag ctggtcgcca gggcgattca cgccagtagc
    26041 gcacgtagcg aaaaaccgct ggtaacgctc aactgtgcgg cactcaacga atccttgctg
    26101 gaatctgaat tgttcggtca cgaaaaaggg gcgttcactg gagccgatag acgccgggaa
    26161 gggcgctttg ttgaggcgga cggcggcacg ctgtttctcg atgaaattgg cgatatctcg
    26221 ccgatgatgc aggtgcgtct gctgcgtgcg attcaggagc gcgaagttca gcgtgtcggt
    26281 agcaaccaga ctatctcggt tgatgtccgg ttgattgcgg cgacccatcg cgatcttgcc
    26341 gcagaggtga atgccgggcg ttttcgccag gatctctact atcgcctgaa tgtggtggcg
    26401 attgaagtac catcgctgcg gcaacggcgg gaagatatcc ctctgctggc tgtccatttt
    26461 cttcagcgct ttgccgagcg taatcgcaag gcggtaaaag gctttacgcc acaggcgatg
    26521 gatttgctga tccactacga ctggccggga aatattcgtg aactggaaaa cgcggtggaa
    26581 cgggcggtgg tgctgctgac cggggaatat atttccgaac gcgagctgcc gctggcgatt
    26641 gccggtacgc cgatcccact ggggcaaagt caggatattc agccgctggt ggaagtcgaa
    26701 aaagaagtga ttctggcggc actggagaaa acgggcggca acaaaaccga agccgcccgt
    26761 cagttaggga tcacgcgcaa aacgctattg gcaaaactgt cgcgttagtt ctgctcgcgt
    26821 tcgatagcgc gccagccgat atctttccgg cagaagcagt cgtcccagtg aatatcggtc
    26881 attaaggcat aagcgcgttt ctgcgcttct gccacggtat gacccagcgc ggtgacgcac
    26941 agtacgcgcc cgccgctggt cactacctgc tcgttatccg ccagtttcgt gcccgcgtgg
    27001 aacactttgc cgcctgccac ttcttccagc ggcaggccgt ggatcacatc gccggtgcgg
    27061 taatcgcccg gatatccacc cgcagccatc accacgccga gagaagcacg ctcatcccac
    27121 tctgacgttt tctcgtccag tttgccttca caggccgcca ggcagagctc aaccagatcg
    27181 gacttcatgc gcagcataat cggctgggtt tctggatcgc caaagcggca gttaaattcg
    27241 ataaccttcg gattgccctg tttgtcgatc atcaggcccg cgtagagaaa accggtgtag
    27301 gtgttgcctt ccgccgccat gcctttcacg gttggccaga tgatacgttc catggtgcgc
    27361 tgatgaactt catcggttac taccggcgcg ggggagtaag cgcccatccc gcccgtgttt
    27421 ggtccggtat ctttatcgcc tacgcgtttg tgatcctggc tggtggccat cggcagcaca
    27481 tgctcgccgt ctaccatcac gataaagctc gcttcttcgc cgtcgaggaa ctcttcgata
    27541 acgatgcgat gacccgcgtc gccaaaagcg ttgcccgcca gcatatcgtg aacagccgct
    27601 tcggcttctt ccagcgtcat cgccacgata acgcctttcc cggctgccag accgtccgct
    27661 ttaatgacga tcggcgcacc tttctcacgc agatacgcca gcgcaggttc tacctcggtg
    27721 aagttctggt attccgctgt agggatctta tggcgggcca ggaaatcttt ggtaaacgct
    27781 ttcgaacctt ccagttgcgc ggcacctgcg gttgggccga agattttcat cccggcggcg
    27841 cggaaggtat cgaccacgcc tttcaccagc ggcgcttccg ggccgacgat ggtcagatca
    27901 atcttttcgt tttgtgcgaa atccaacagc gccgggatat cggtcacgcc gatagcaacg
    27961 ttttgcagcg cgggttccag cgcagtgcct gcattacccg gagcaacaaa aacagtttca
    28021 accagcggcg actgcgccgc tttccaggcc agcgcgtgct cgcgcccgcc gttaccaatc
    28081 actaatactt tcatctattg ctccattaat ggcggaagtg gcgcatgtcg gtgaagagca
    28141 tcgcaatacc gtgctcgtcg gcggcggcaa tcacttcgtc atcacggata gaaccgccag
    28201 gctggattac gcaggtcacg cctgcagcgg cggcggcatc aataccgtcg cggaacggga
    28261 agaacgcgtc agaagccatc gaggaacctt tcacttccag gccttcatcg gcagctttaa
    28321 taccggcgat tttcgcggag tacacgcggc tcatctggcc cgcgccaatc ccgatggtca
    28381 tattgttttt ggcatagacg atagcgttgg atttcacgaa cttcgccact ttccagcaga
    28441 acagcgcatc acgcagttcc tgttcggtcg gctgacgttg ggtgacgacg cgcagttctt
    28501 ccgcaccgac catgcccagg tcacgatcct gaaccagcag accgccgttc acgcgtttga
    28561 aatcaagacc cggaacacgc tcgccccact gaccgcaggt cagaacgcgt acgttctgct
    28621 tggcggcggt gattttcagg gcttcttcgc tggcggaagg cgcaataatc acttcgacaa
    28681 actgacgaga aatgatggcc tgtgcggttt ccgcatccag ctcgcggtta aaggcaatga
    28741 tgccgccgaa tgcggaggtt gggtcggttt tgtacgcgcg atcgtaagca tcaagaatgg
    28801 aattgccgat agccacgccg caagggttgg cgtgcttcac aatcacacat gccggctcgg
    28861 cgaactcttt cacgcactcc agcgccgcat cggtatcggc gatgttgtta taagagaggg
    28921 ctttaccctg aacctgggtt gcggtagcaa cggaggcttc tttcacattc tcttctatat
    28981 agaaggcagc ctgctggtgg ctgttctcgc cgtaacgcat atcctgcttc ttaatgaagt
    29041 tcaggttcag cgtgcgtggg aagcgaccgg cggcttcttt gctttcaccg tggtaagccg
    29101 gaaccatgct gccgaagtag ttggcaatca tgctgtcgta ggcggcagtg tgttcgaagg
    29161 ctttgatggc gaggtcgaaa cgggttgcaa gcgtcagcga tccttcgttg tcatccatct
    29221 ctttaataat ggcgtcgtag tcgctgctct tcaccacgat tgcgacatct ttatggttct
    29281 tagcggcgga gcgcaccatg gtcgggccgc cgatatcgat gttctcaacc gcatcttcca
    29341 gcgagcagcc ttcacgagca acagtctggg cgaacggata caggttaaca accaccatat
    29401 cgataggctg gatctgatgt tcttccataa tggcatcgtc ctggccgcga cggcccagaa
    29461 tgccaccatg tactttcgga tgcagggtct tcacgcgtcc atccatcatc tccgggaaac
    29521 cggtgtaatc ggaaacttcg gttaccggca gacctttttc tgctaacaga cgggcagtgc
    29581 cccctgtaga cagcagctcc acaccgcgtg cggaaagtgc ctgggcgaat tcgacgatac
    29641 cggctttgtc agaaacactg agcagagcgc ggcggactgg acgacgttgt tgcatggtaa
    29701 atcccctgga tttgactatt acagagagcg ttagctgaat ttttcgcgaa aaactcagct
    29761 aacgccccta acggggcatc cttatttttc gcccgcattg taacgaaaac gtttgcgcaa
    29821 cgctcgcgaa tttttctctt tcaatggtga tcacaatttt gacagtggtt accgtgggaa
    29881 aaatacagaa attacattga tgattgtgga taactctgtg cgtaaaaagg tataaagcgg
    29941 ggttttgctg gggaatgcag cagtcagtca tttttctgca atttttctat tgcggcctgc
    30001 ggagaactcc ctataatgcg cctccatcga cacggcggat gtgaatcact tcacacaaac
    30061 agccggttcg gttgaagaga aaaatcctga aattcagggt tgactctgaa agaggaaagc
    30121 gtaatatacg ccacctcgcg acagtgcgct aaag
//
LOCUS       DAESHI010000044        29548 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.044, whole genome shotgun sequence.
ACCESSION   DAESHI010000044 DAESHI010000000
VERSION     DAESHI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.044"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..689
                     /gene="dgt"
                     /locus_tag="KW011_002041"
     CDS             <1..689
                     /gene="dgt"
                     /locus_tag="KW011_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HBH7660374.1"
                     /translation="EDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRS
                     NSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLL
                     KLYKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRF
                     PIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWD
                     EYRRLMAVEQ"
     gene            819..2243
                     /gene="degP"
                     /locus_tag="KW011_002042"
     CDS             819..2243
                     /gene="degP"
                     /locus_tag="KW011_002042"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HBH7660375.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2398..3555
                     /gene="cdaR"
                     /locus_tag="KW011_002043"
     CDS             2398..3555
                     /gene="cdaR"
                     /locus_tag="KW011_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HBH7660376.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3644..4030)
                     /locus_tag="KW011_002044"
     CDS             complement(3644..4030)
                     /locus_tag="KW011_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HBH7660377.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="KW011_002045"
     CDS             complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="KW011_002045"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HBH7660378.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="KW011_002046"
     CDS             complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="KW011_002046"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HBH7660379.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="KW011_002047"
     CDS             complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="KW011_002047"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HBH7660380.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8648..9442)
                     /gene="map"
                     /locus_tag="KW011_002048"
     CDS             complement(8648..9442)
                     /gene="map"
                     /locus_tag="KW011_002048"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBH7660381.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9810..10535
                     /gene="rpsB"
                     /locus_tag="KW011_002049"
     CDS             9810..10535
                     /gene="rpsB"
                     /locus_tag="KW011_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBH7660382.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10793..11644
                     /gene="tsf"
                     /locus_tag="KW011_002050"
     CDS             10793..11644
                     /gene="tsf"
                     /locus_tag="KW011_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBH7660383.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11791..12516
                     /gene="pyrH"
                     /locus_tag="KW011_002051"
     CDS             11791..12516
                     /gene="pyrH"
                     /locus_tag="KW011_002051"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBH7660384.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12808..13365
                     /gene="frr"
                     /locus_tag="KW011_002052"
     CDS             12808..13365
                     /gene="frr"
                     /locus_tag="KW011_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBH7660385.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13457..14653
                     /gene="ispC"
                     /locus_tag="KW011_002053"
     CDS             13457..14653
                     /gene="ispC"
                     /locus_tag="KW011_002053"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBH7660386.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14842..15600
                     /gene="ispU"
                     /locus_tag="KW011_002054"
                     /gene_synonym="uppS"
     CDS             14842..15600
                     /gene="ispU"
                     /locus_tag="KW011_002054"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HBH7660387.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15613..16470
                     /gene="cdsA"
                     /locus_tag="KW011_002055"
     CDS             15613..16470
                     /gene="cdsA"
                     /locus_tag="KW011_002055"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBH7660388.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16482..17834
                     /gene="rseP"
                     /locus_tag="KW011_002056"
     CDS             16482..17834
                     /gene="rseP"
                     /locus_tag="KW011_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HBH7660389.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17864..20296
                     /gene="bamA"
                     /locus_tag="KW011_002057"
     CDS             17864..20296
                     /gene="bamA"
                     /locus_tag="KW011_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HBH7660390.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20418..20903
                     /gene="skp"
                     /locus_tag="KW011_002058"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20418..20903
                     /gene="skp"
                     /locus_tag="KW011_002058"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HBH7660391.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20907..21932
                     /gene="lpxD"
                     /locus_tag="KW011_002059"
     CDS             20907..21932
                     /gene="lpxD"
                     /locus_tag="KW011_002059"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HBH7660392.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22037..22492
                     /gene="fabZ"
                     /locus_tag="KW011_002060"
     CDS             22037..22492
                     /gene="fabZ"
                     /locus_tag="KW011_002060"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBH7660393.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22496..23284
                     /gene="lpxA"
                     /locus_tag="KW011_002061"
     CDS             22496..23284
                     /gene="lpxA"
                     /locus_tag="KW011_002061"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HBH7660394.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23284..24432
                     /gene="lpxB"
                     /locus_tag="KW011_002062"
     CDS             23284..24432
                     /gene="lpxB"
                     /locus_tag="KW011_002062"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HBH7660395.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24429..25025
                     /gene="rnhB"
                     /locus_tag="KW011_002063"
     CDS             24429..25025
                     /gene="rnhB"
                     /locus_tag="KW011_002063"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBH7660396.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25062..28544
                     /gene="dnaE"
                     /locus_tag="KW011_002064"
     CDS             25062..28544
                     /gene="dnaE"
                     /locus_tag="KW011_002064"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBH7660397.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28557..29516
                     /gene="accA"
                     /locus_tag="KW011_002065"
     CDS             28557..29516
                     /gene="accA"
                     /locus_tag="KW011_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBH7660398.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7159 a         7121 c         8160 g         7108 t
ORIGIN      
        1 ttgaagatgc ggtagagaaa agaatattta ccgttgagca gctttatcat catttgcacg
       61 aggcgtgggg tcagcatgag aaaggttcgc tcttttcgct agtggttgaa aatgcctggg
      121 aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga tcagtttttt atgtatttac
      181 gggtaaacac cctaaataaa ctggtaccct acgcggcaca acgatttatt gataatctgc
      241 ctgcgatttt cgccggaacg tttaatcatg cattattgga agatgccagc gaatgcagcg
      301 atcttcttaa gctatataaa aatgtcgctg taaaacatgt gtttagccat ccggatgtcg
      361 agcagcttga attgcagggc tatcgggtca ttagcggatt attagagatt tatcgtcctt
      421 tattaagcct gtcgttatca gactttactg aactggtaga aaaagaacgg gtgaaacgtt
      481 tccctattga gtcgcgctta ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg
      541 aggctgtcag taaattaccg tcagattctc ctgagtttcc gctatgggaa tattattacc
      601 gttgccgcct actgcaggat tatatcagcg gtatgaccga cctctatgcg tgggatgaat
      661 accgacgtct gatggccgta gaacaataac caggcttttg taaagacgaa caataaattt
      721 ttaccttttg cagaaacttt agttcggaac ttcgcgctat aaaacgaatc tgaagaacac
      781 agcaattttg cgttatctgt taatcgagac tgaaatacat gaaaaaaacc acattagcac
      841 tgagtgcact ggctctgagt ttaggtttgg cgttatctcc gctctctgca acggcggctg
      901 agacttcttc agcaacgaca gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg
      961 tgatgccttc agtggtcagc attaacgtag aaggtagcac aaccgttaat acgccgcgta
     1021 tgccgcgtaa tttccagcag ttcttcggtg atgattctcc gttctgccag gaaggttcac
     1081 cgtttcagag ctctccgttc tgccaggggg gccagggcgg taatggcggc ggccagcaac
     1141 agaaattcat ggcgctgggt tccggcgtca tcattgatgc cgataaaggc tatgtcgtca
     1201 ccaacaacca tgttgttgat aacgcgacgg tgattaaagt ccagctgagc gatggtcgta
     1261 agttcgacgc gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg atccaaatcc
     1321 agaacccgaa aaacctgacc gcaattaaga tggcggattc tgatgcactg cgcgtgggtg
     1381 attacaccgt agcgattggt aacccgtttg gtctgggcga gacggtaact tccgggattg
     1441 tctctgcgct ggggcgtagc ggcctgaatg ctgaaaacta cgaaaacttc atccagaccg
     1501 atgcagcgat caaccgtggt aactccggtg gtgcgctggt taacctgaac ggcgaactga
     1561 tcggtatcaa caccgcgatc ctcgcaccgg acggcggcaa catcggtatc ggttttgcta
     1621 tcccgagtaa catggtgaaa aacctgacct cgcagatggt ggaatacggc caggtgaaac
     1681 gcggtgagct ggggattatg gggactgagc tgaactccga actggccaaa gcgatgaaag
     1741 ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag
     1801 cgggcattaa agcgggtgat gtgatcacct cactgaacgg taagccgatc agcagctttg
     1861 ccgcactgcg tgctcaggtg ggtactatgc cggtgggcag caaactgacc ctgggcttac
     1921 tgcgcgacgg taagcaggtt aacgtgaacc tggaactgca gcagagcagc cagaatcagg
     1981 ttgattccag ctccatcttc aacggcattg aaggcgctga gatgagcaac aaaggcaaag
     2041 atcagggcgt ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga
     2101 agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc
     2161 gtaaagtgct cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc ggcgacagca
     2221 ccatctacct gttaatgcag taatctccct caaccccttc ctgaatacgg gaaggggttc
     2281 tccttacaat ctgtgaactt cgccacaact ccatacatct tcatcatcct ttgggcattt
     2341 gcacaatgcc gtacgttacg tacttcctta tgctaagccg tgcataacgg aggacttatg
     2401 gctggctggc atcttgatac caaaatggcg caggatatcg tggcacgtac catgcgcatc
     2461 atcgatacca atatcaacgt aatggatgcc cgcgggcgaa ttatcggcag cggcgatcgt
     2521 gagcgtattg gtgaattgca cgaaggtgca ttgctggtac tttcacaggg acgagtcgtc
     2581 gatatcgatg acgcggtagc acgtcatctg cacggtgtgc ggcaggggat taatctaccg
     2641 ttacggctgg aaggtgaaat tgtcggcgta attggcctga caggtgaacc agagaatctg
     2701 cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca gtcgcggttg
     2761 atgcacctgt tggcgcagga tagccgtttg cgggaagaac tggtgatgaa cctgattcag
     2821 gctgaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat tgatctcaat
     2881 caaccgcgag tggtggctat tgttgaggtc gacagcggtc agcttggcgt ggacagcgca
     2941 atggcggagt tacaacaact gcaaaatgcg ctgactacgc ccgagcgtaa taatctggtg
     3001 gcaattgttt cgctgaccga aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc
     3061 tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat gaaagagtac
     3121 ggccagctgc gttttcgcgt ttcactgggc aactatttta ccggtcctgg cagtattgcc
     3181 cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat gccagaaagt
     3241 cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt gcgtggcgac
     3301 tggcaggcca acgaactggc gcgaccgctg gcgcggctga aagcgatgga caataatggc
     3361 ttgctgcgac gaacgctggc ggcgtggttt cgccacaatg tgcaaccgct ggctacgtca
     3421 aaggcgctgt ttattcatcg taatactctg gagtatcggc ttaatcgtat atcggaactg
     3481 accgggcttg atttgggtaa ttttgatgac aggttgctgc tgtatgtggc gttacaactg
     3541 gatgaagagc ggtaggttat gcgttaaggt cgaggcggcg ctggctcatt cccctcagcc
     3601 cgaagggtag aggggaatgt ccgagttaac cgtcagttcg gcattattta cgcgttagtt
     3661 tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc aggtgacgta
     3721 agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg cagatttgat
     3781 caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct ttataaccct
     3841 gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac ttcacgctct
     3901 tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg ttgttggctt
     3961 cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga cttttcagat
     4021 tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt agcatttggc
     4081 atggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac ttctatcatg
     4141 gcgtaaaaat caaaaaatta cctgctttat tgtggtgata aaattcacgt tctacaccag
     4201 ttccagcatc gttacttccg ggcggcagtt cagacgcaaa ccatacaaac tgcccacgcc
     4261 acgggttgtg tagatgtgtc gttcgccaaa ggcatttaat ccggcgacgt aacgtttatc
     4321 ttcgacaggg gcaaaaggtt caccaaccaa cggtacgcgc agttgcccgc cgtgagtatg
     4381 gccgcacagc atcagatccc agggttcatc acgcatgact tctttgctgt cgggattatg
     4441 tgccagcatc agtcttggca gatttgcttc gctggcggga ggtggtttgc attgtccggc
     4501 ccataagtca ccggtgccta ccagttcgaa ttgcctgttc ggcgtggcga tcaccgtagc
     4561 ctggttaaac aacaccgtga tgcccactga tttcaacgtc tcgccaatta agtgattttt
     4621 ttctgtacca acagggcgat cgtggttgcc gaagcaggca aacgtcggcg cacattcggc
     4681 aaggggggag aggacatcac taaacgccga aaaattcagc ggcatatcaa ataatacgta
     4741 atcgccgccc agtaatatca aatcgggctt ttgttctatg ccaagagcaa tcgcgtcaga
     4801 aatcaggctt aaaggaacaa aacgagagta atggagatcg gccagaaaaa gaattttgaa
     4861 tggtgctgcg ttgtctttaa aaaaggcgag gcggtgacgg attaattcaa accagcctag
     4921 ctcccagtaa tgcatataac cgaaacctga gctcgtggct atcgtcgcgg cagtagcctg
     4981 caacaatcgg cggcgtgaaa tcatcgctca tccctgcaaa aaaaatcggg cagcgtcgtg
     5041 ctgcccgtgt gcatactttt agtcgatggt acgcagcagt tcgttaatgc cgactttgcc
     5101 gcgagttttc gcgtcaactt tcttaacgat aaccgcacag tagaggctgt atttgccatc
     5161 ttttgacggc agattacctg aaacaaccac agaccccgcc ggaacgcgac cgtagtggat
     5221 ttcgccggtt tcacggtcgt aaatacgggt gctctgacca atgtatacgc ccatggaaat
     5281 gacggaacct tcttcgacaa tcaccccttc aaccacttca gagcgtgcgc caatgaagca
     5341 gttatcttca atgatggttg ggttagcctg cagcggttcc agcacgccgc cgatgcccac
     5401 gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt
     5461 atcaaccatg gtgccttcat caacatatgc gccgatgttg acgtaagacg gcatcagcac
     5521 ggtgttacgg gcaataaacg caccctgacg taccgccgct ggtggcacaa cgcggaagcc
     5581 ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc atcggcactt tgtcgaagta
     5641 gcggctttct gccccttcga tcacctgatt atcattaata cggaaagaga gcagcaccgc
     5701 ttttttcaac cactgatgcg tcacccactg accgtcaatt ttttccgcta cacgcagtgc
     5761 accggaatcc agcagggcga tcacctgatt taccgcttcg cgggtaacgg tgtctgcgtt
     5821 ggctggcgtg atctcggcac ggcgttcaaa agcggtttca ataatgttct gtaactgctg
     5881 cattgttaaa ctcttttcat atcagtaaac acatcaccct ttatcgtttg gattgagggc
     5941 ctctgtcaac cgctgatgca cttcctgctg caactcgtta ttaagtgcac gccggtcagc
     6001 ggtggcaatt atgaataaat cttctactcg ctcgccaatg gttgtaattc gggcaccatg
     6061 aagcgaaatt cccagatcgg caaaaatttt cccgactctc gccagcagtc ctggctggtc
     6121 gagcgcgata agctcaagga acgatttgcg gtcggtatgg gttggcaaaa acgtcacttc
     6181 ggtttcaaca gtaaagtgac gtagtttagc cggttgacga cgcggctgtg gtggctgcca
     6241 gctactttgt gtcagtacct gctccagacc aaaccgaata acctcatgac gatctgcgga
     6301 tagcgggcta ccatcgggtt ccagcacgat gaaggtatcc atcgccatac cgtcgcgagt
     6361 ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg cacagacggc
     6421 ggcaaacaga taggggcggt ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc
     6481 ctgcgggcta agcaatacca gcggtttgct taaatcatgc tgtaataaat ggcgggcatg
     6541 ccaggccagt tgatttgggc tatggcggac aaaatagtta gcacgacagc gtgaccaaat
     6601 ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt agtgccagtg cctggagttg
     6661 gtgatggcga acccgttcgc gcatatccgg cgtgttttgc attccgcgtc gtagctgctt
     6721 ttcggtggca aaatagagtt cgcgcaacag actttgcttc cagctattcc acagcgtttc
     6781 gttggtggcg caaatgtcag ccactgtcag gcataccaga tagtgcagac gattttccgt
     6841 ttgaacttct tcggcaaact gcttgatgac ttcagggtcc tgaatatcgc ggcgttgtgc
     6901 tgtcaccgac atcagcaagt gctggcgaac cagccaggca accagctgtg tttcgcgtga
     6961 gttcagcccg tggagttctg caaaatgcac tacatcctga gcaccgagaa tggagtggtc
     7021 gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga
     7081 cggcaggcgc ggccagacgt ccacacacaa cggatggcgc tggcgcgttt cttcactggc
     7141 aaaactctcc agtttcagca tcacgcggat agtatgttca tccaccgtgt aggcgtggaa
     7201 cagatcaaac tgcatctgcc cgacgatatg cgaccattgc ggcatatacg cgccgagcac
     7261 gctatggcga tgcattggca atagcccgcg ccgcaccgct ccggggtgac gcagaatgct
     7321 caaaaacagt tttcgtgctt ccggaatatt acacagcggt tgttgcagat ggcgacgggc
     7381 atggcgtaac tggcgcagcg tggtggagta aatgccggtg atcgcactgt tgcgcaccat
     7441 ggtgtagaac atacgcaaga tggcttccgg ctggcgcata aatagtgttt catcacgcag
     7501 gtcgattagc gtaccgcgta gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc
     7561 gggaagggcg aggatggctt catcgaacag ttgcagcagc atctggttga gttcactgac
     7621 gcggcgtgta acgcggaagt aatccttcat catccgctcg accggttcgt tgccttcacc
     7681 actgtaattc agacgctgag cgacgctaag ttggcgatcg aataacaggc gattatcgta
     7741 acggctgacg accagatgca gggcaaagcg aatacgccac agtatatgca gacattcgtt
     7801 taattccgcc cgctccgctg aggttaagaa gccaaaccca accatttcat ccagcgatgt
     7861 tgcgccaaaa tgacggcggg ccacccattg cagagtgtgg atatcgcgca agccgccagg
     7921 gctacttttg atgtctggtt caagattgta gctggtgccg tggtaacgct gatggcgctg
     7981 gttctgttct tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa
     8041 aatatgtttt tgcagttcga ggaagagcgc aacatcgcca attaataagc gggattcgat
     8101 taaattggtg gcgacggtta aatccgataa cccttccagc atgcactctt caagcgtgcg
     8161 cacactatga ccgacttcca gctttacatc ccagagcagc gttaacagct cgcccacttt
     8221 ttgcgcctga tcgtccggga gctttttacg gcttaaaatc agtaaatcga tgtcagaaag
     8281 tggatgcagc tcgccacgac cgtagccacc gacggcgacc aatgccaggt cggcaatctg
     8341 gctgaatccc gcttcaatcc ataatcgttg caggagctgg tcgataaact cggtgcgcgc
     8401 ctcaatcaac tgttctgcag agatcccatt gtcaaaggca tcacccagcc aacgctggaa
     8461 agtatcgata tgggctttta tcccaccgac ggttaattca tcacggggcc agacgcatgg
     8521 attttgcggt tgaccgggca gggtggggag agcggtgttt gcgtactgtt ctggaagggt
     8581 attcattgtg cgccacccat aagattaaat tatcgcatta aaaaagccgg cattcgccgg
     8641 cttcatctta ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg cgtagcgtca
     8701 gaatttcgca gccgttatca gtcaccacaa tagtatgctc atattgtgca gacaagctgc
     8761 gatctttggt ttttaccgtc cagccatctt tcatggtgcg gatctctttt ttacccgcgt
     8821 tgaccattgg ctcgatggtg aacgtcatcc caggtttcag tacgacgttg gtttcacggg
     8881 agtcatagtg cagcacctgc ggttcttcat ggaagccgcg accaataccg tgtccgcaat
     8941 attcacgaac gacggagaag ccttctgctt cgacaaactt ctgaatcgcc gcaccgattt
     9001 cgcgcaggtt aatgcctggt ttgaccatgc gcagcgccag gtacaggctt tcttgcgtga
     9061 tgcggcacag acgttcgccc atgatggtcg gcttaccgac gataaacatt ttcgaggtat
     9121 cgccgtggaa accatctttg attacggtga catcaatgtt aacgatatcg ccatctttca
     9181 gcagcttcgc atcgtccggg ataccgtggc acaccacttc attaatagag atgcaaacgg
     9241 atttcggata gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt
     9301 aatcattaca gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca tacggttcga
     9361 tcatctccag cacttcggca gccagtcggc cagcgacgcg cattttttcg atatcttctg
     9421 gggtcttgat tgagatagcc attaattctg tccatcagcg tcggtgatac cgacaatata
     9481 tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg agaatcattc tgaatttcgc
     9541 caaacgtgcc actgaacgtt ttctataata gaaaattcaa cgtctgatgc tgtacacagc
     9601 gccaacaatt attggtgtcc atgacgtatt tgtggtataa agcgcgccgg acttccgatc
     9661 catttcgtat acacagactg gacggaagcg acaatctcac tttgtgtaac aacacacacg
     9721 tatcggcaca tattccgggg tgccctttgg ggtcggtaat atgggatacg tggaggcata
     9781 accccaactt ttatatagag gttttaatca tggcaactgt ttccatgcgc gacatgctca
     9841 aggctggtgt tcacttcggt caccagaccc gttactggaa cccgaaaatg aagccgttca
     9901 tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga gaaaactgta ccgatgttca
     9961 acgaagctct ggctgaactg aacaagattg cttctcgcaa aggtaaaatc cttttcgttg
    10021 gtactaaacg cgctgcaagc gaagcggtga aagacgctgc tctgagctgc gaccagttct
    10081 tcgtgaacca tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca
    10141 tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg tactttcgaa aagctgacca
    10201 agaaagaagc gctgatgcgc actcgtgagc tggagaaact ggaaaacagc ctgggcggta
    10261 tcaaagacat gggcggtctg ccggacgctc tgtttgtaat cgatgctgac cacgaacaca
    10321 ttgctatcaa agaagcaaac aacctgggta ttccggtatt tgctatcgtt gataccaact
    10381 ctgatccgga cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga
    10441 ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg ccgttctcag gatctggctt
    10501 cccaggcgga agaaagcttc gtagaagctg agtaataagg cttgataact cccccaaaat
    10561 agttcgagtt gcagaaaggc ggcaagctcg agaattcccg ggagcttaca tcagtaagtg
    10621 accgggatga gcgagcgaag acaacgcatc tgcggcgcga aatatgaagg gggagagccc
    10681 ttatagacca ggtagtacac gtttggttag ggggcctgca tatggccccc tttttcactt
    10741 ttatatctgt gcggtttaat gccgggcaga tcacatctcc gaggatttta gaatggctga
    10801 aattaccgca tccctggtaa aagagctgcg tgagcgtact ggcgcaggca tgatggattg
    10861 caaaaaagca ctgactgaag ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa
    10921 gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa
    10981 aaccaaaatc gacggcaact acggcatcat tctggaagtt aactgccaga ctgacttcgt
    11041 tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg
    11101 caaaatcact gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt
    11161 agcgaaaatt ggtgagaaca tcaacattcg ccgcgttgct gcgctggaag gcgacgttct
    11221 gggttcttat cagcacggtg cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga
    11281 agagctggtt aaacacatcg ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc
    11341 agaagacgta tccgctgaag tggtagaaaa agaataccag gtacagctgg atatcgcgat
    11401 gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt
    11461 caccggcgaa gtttctctga ccggtcagcc gttcgttatg gaaccaagca aaactgttgg
    11521 tcagctgctg aaagagcata acgctgaagt gactggcttc atccgcttcg aagtggggga
    11581 aggcatcgag aaagttgaga ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc
    11641 ttaattatcg aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc
    11701 agctaaccct tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt attaattcat
    11761 ttcaatcgtt gacagtctca ggaaagaaac atggctacca atgcaaaacc cgtctataaa
    11821 cgcattctgc ttaagttgag tggcgaagct ctgcagggca ctgaaggctt cggtattgat
    11881 gcaagcatac tggatcgtat ggctcaggaa atcaaagaac tggttgaact gggtattcag
    11941 gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt
    12001 atgaaccgcg ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa cggcctggca
    12061 atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc tattccattg
    12121 aatggcgtgt gcgacagcta cagctgggca gaagctatca gcctgttgcg caacaaccgt
    12181 gtggtgatcc tctccgccgg tacaggtaac ccgttcttta ccaccgactc agcagcttgc
    12241 ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg
    12301 tttaccgctg atccggcgaa agatccaacc gcaaccatgt acgagcaact gacttacagc
    12361 gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac
    12421 cataaattac cgattcgtgt tttcaatatg aacaaaccgg gtgcgctgcg ccgtgtggta
    12481 atgggtgaaa aagaagggac tttaatcacg gaataattcc cgtgatggat aaataagggt
    12541 aagattccgc gtaagtatcg cgggggcgta agtctggtta taaggcgtta ttgttgcagg
    12601 cagtttggtc acggccagcg cgcagcaacc ggagcgtaca aaagtacgtg aggatggcga
    12661 gcactgctcg gggccaaaat ggcaaataaa atagcctaat aatccagacg attacccgta
    12721 atatgtttaa tcagggctat acttagcaca cttccactgt gtgtgactgt ctggtctgac
    12781 tgagacaagt tttcaaggat tcgtaacgtg attagcgata tcagaaaaga tgctgaagta
    12841 cgcatggaca aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt
    12901 gcttctccca gcctgctgga tggcattgtc gtggaatatt acggcacgcc gacgccgctg
    12961 cgtcagctgg caagcgtaac ggtagaagat tcccgtactc tgaaaatcaa cgtgtttgat
    13021 cgttcaatgt ctccggccgt tgaaaaagcg attatggcgt ccgatcttgg cctgaacccg
    13081 aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga acgtcgtaaa
    13141 gatctgacca aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt acgtaacgtg
    13201 cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag ataaagagat cagcgaagac
    13261 gacgatcgcc gttctcagga cgatgtacag aaactgactg atgctgcaat caagaaaatt
    13321 gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt tctgatttct tgaacgacaa
    13381 aaacgccgct cagtagatcc ttgcggatcg gctggcggcg ttttgctttt tattctgtct
    13441 caactctgga tgtttcatga agcaactcac cattctgggc tcgaccggct cgattggttg
    13501 cagcacgctg gacgtggtgc gccataatcc cgaacacttc cgcgtagttg cgctggtggc
    13561 aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa ttctctcccc gctatgccgt
    13621 aatggacgat gaagcgagtg cgaaacttct taaaacgatg ctacagcaac agggtagccg
    13681 caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg gcagcgcttg aggatgttga
    13741 tcaggtgatg gcagccattg ttggcgctgc tgggctgtta cctacgcttg ctgcgatccg
    13801 cgcgggtaaa accattttgc tggccaataa agaatcactg gttacctgcg gacgtctgtt
    13861 tatggacgcc gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg aacataacgc
    13921 catttttcag agtttaccgc aacctatcca gcataatctg ggatacgctg accttgagca
    13981 aaatggcgtg gtgtccattt tacttaccgg gtctggtggc cctttccgtg agacgccatt
    14041 gcgcgatttg gcaacaatga cgcccgatca agcctgccgt catccgaact ggtcgatggg
    14101 acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa ggtctggaat acattgaagc
    14161 gcgttggctg tttaacgcca gcgccagcca gatggaagtg ctgattcacc cgcagtcagt
    14221 gattcactca atggtgcgct atcaggacgg cagtgttctg gcgcagctgg gggaaccgga
    14281 tatgcgtacg ccaattgccc acaccatggc atggccgaat cgcgtgaact ctggcgtgaa
    14341 gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt atgatcgtta
    14401 tccatgcctg aaactggcga tggaggcgtt cgaacaaggt caggcagcga cgacagcatt
    14461 gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg caacaaatcc gctttacgga
    14521 tatcgctgcg ttgaatttat ctgtactgga aaaaatggat atgcgcgaac cacaatgtgt
    14581 ggacgatgtg ttatctgttg atgcgaacgc gcgtgaagtc gccagaaaag aggtgatgcg
    14641 tctcgcaagc tgaggataat ccggctacag agagtcgcgc tatttgttag cgtagggctt
    14701 cagtgatata gtctgcgcca tctgatcgta agtagttggc tttataaggt caggtatgcc
    14761 gtggttttac acggcttttt tttgtatagg cttcagtatt cctgagtacc gtaaaccctg
    14821 tcagggaata aaaaacgcgt gatgttgtct gctactcaac cacttagcga aaaattgcca
    14881 gcgcatggct gccgtcatgt tgcgatcatt atggacggca atggtcgctg ggcaaaaaag
    14941 caagggagga ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg ccgggctgtc
    15001 tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt atgcctttag tagtgaaaac
    15061 tggaaccgac cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg ggcgctcgat
    15121 agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc gtattattgg cgataccagt
    15181 cgctttaact cgcgtttgca agaacgtatt cgtaaatctg aagcgctaac agctgggaat
    15241 accggtctga cgctgaatat tgcggcgaac tacggtggac gttgggatat agtccaggga
    15301 gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca gatagatgaa
    15361 gagatgctaa accagcatgt ctgtatgcat gaactggccc ctgtagattt agtaattagg
    15421 actggggggg agcatcgcat tagtaacttt ttgctttggc aaattgccta tgccgaactt
    15481 tactttacag atgttctctg gcccgatttc gatgaacaag actttgaagg ggcgttaaat
    15541 gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga aacagcctga
    15601 tggggggcgc ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat acccgtcgtc
    15661 atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca tcgtaacgct ggtggtctgc
    15721 atgctggcag cgtgggaatg gggacagctt agcggtttta ccactcgttc gcagcgagta
    15781 tggttggcgg tgttatgcgg gttattgttg gcgctgatgc tttttctgtt gccggaatat
    15841 caccgaaata ttcatcaacc gctggttgaa atctcacttt gggcttcgtt gggttggtgg
    15901 attgtcgcgc tattgctggt gctgttttac ccaggttccg cagcaatctg gcgtaactct
    15961 aaaacattgc gccttatttt tggcgtgcta accattgttc ccttcttctg gggcatgctg
    16021 gcgttacggg cctggcacta tgacgagaat cattacagtg gcgcaatatg gctgctctat
    16081 gtcatgatcc tggtatgggg cgctgactcc ggcgcatata tgtttggcaa attgtttggt
    16141 aaacataagc tggcaccgaa ggtttctccg ggtaaaacct ggcaaggctt tataggtgga
    16201 ctagcgaccg cagcggtaat ctcatggggt tatggcatgt gggcgaatct cgacgtcgct
    16261 cccgtcacct tactcatttg ctctattgtc gcagcgttag cctcagtgct cggcgatctg
    16321 accgagagta tgtttaagcg tgaagcagga attaaggaca gcggtcattt aattccagga
    16381 cacggtggta ttttagatcg tattgatagc ctgacggctg cggtaccggt ctttgcttgc
    16441 ttgttgttac tggtattcag gacgctttaa cggaaggtaa tatgctgagt tttctctggg
    16501 atttggcttc gttcatcgtt gcactgggtg tacttatcac cgtgcatgaa tttggtcatt
    16561 tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg tttggtaagg
    16621 cgctctggcg gcgaactgat aagctcggca ccgaatatgt tatggccctg attccgctgg
    16681 gcggttatgt caaaatgctg gatgagcgcg cagaaccggt cgttccggaa ctccgccacc
    16741 atgccttcaa taataaatct gttggccaac gagcggcgat tattgccgca ggtccggttg
    16801 caaacttcat ttttgctatc tttgcctact ggctggtttt tattattggt gtgcctggcg
    16861 tacgtccggt ggttggtgaa atagcagcca attcgatagc tgcggaagca caaattgcac
    16921 caggtacgga actaaaagcc gtagatggta tcgaaacgcc tgattgggat gccgtgcgtt
    16981 tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat tacagtagcg ccatttggca
    17041 gcgaccaacg gcgggatgta aagctcgatt tacgtcactg ggcgtttgag cctgataaag
    17101 aagatccggt atcttcgctg gggattcgtc ctcgtgggcc gcaaattgaa cctgtactgg
    17161 aaaatgtgca gccaaactcg gcggcaagca aggcaggttt gcaagcaggc gacaggatcg
    17221 ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt gtccgggata
    17281 acccgggtaa atccttagcg ttagaaatcg aaaggcaggg gagtcccttg tctttgacat
    17341 taatcccgga gagtaaaccg ggtaatggta aagcgattgg ttttgtcggt attgagccga
    17401 aagtcattcc tttgccagat gagtataaag ttgtacgcca gtatgggccg ttcaacgcca
    17461 tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct gacggtcagt atgctgggaa
    17521 aattgatcac cggtgatgtg aaactgaaca acctcagtgg gccgatctct atcgccaagg
    17581 gggctgggat gacagcggaa ctcggggtag tttattacct gccgtttctt gcgcttatta
    17641 gcgtgaactt agggataatt aacctgtttc cgttgcccgt acttgacggg gggcatctgc
    17701 tgttccttgc gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt caagactttt
    17761 gttatcgcat tggctcgatt ctgctggtgc tgttaatggg gcttgcactt ttcaatgatt
    17821 tctctcggtt atgagagtta gttaggaaga acgcataata acgatggcga tgaaaaagtt
    17881 gctcatagcg tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag ggttcgtagt
    17941 gaaagatatt catttcgaag gccttcagcg tgtcgccgtt ggtgcggcac tcctcagtat
    18001 gccggtgcgc acaggcgaca cggttaatga tgaagatatc agtaatacca ttcgcgctct
    18061 gtttgctacc ggcaactttg aggatgttcg cgtccttcgt gatggtgata cccttctggt
    18121 tcaggtaaaa gaacgtccga ccattgccag cattactttc tccggtaaca aatcggtgaa
    18181 agatgacatg ctgaagcaaa acctcgaggc ttctggtgtg cgtgtgggcg aatccctcga
    18241 tcgcaccacc attgccgata tcgagaaagg tctggaagac ttctactaca gcgtcggtaa
    18301 atatagcgcc agcgtaaaag ctgtcgtgac cccgctgccg cgcaaccgtg ttgacctaaa
    18361 actggtgttc caggaaggtg tgtcagctga aatccagcaa attaacattg ttggtaacca
    18421 tgctttcacc accgacgaac tgatctctca tttccaactg cgtgacgaag tgccgtggtg
    18481 gaacgtggta ggcgatcgta aataccagaa acagaaactg gcgggcgacc ttgaaaccct
    18541 gcgcagctac tatctggatc gcggttatgc ccgtttcaac atcgactcta cccaggtcag
    18601 tctgacgcca gataaaaaag gtatttacgt cacggtgaac attaccgaag gcgatcagta
    18661 caagctttct ggcgttgaag tgagcggcaa ccttgccggg cactccgctg aaattgagca
    18721 gctgactaag atcgagccgg gcgagctgta taacggcacc aaagtgacca agatggaaga
    18781 tgacatcaaa aagcttctcg gtcgctatgg ttatgcctat ccgcgcgtac agtcgatgcc
    18841 tgaaattaac gatgccgaca aaaccgttaa attacgcgtg aacgttgatg cgggtaaccg
    18901 tttctacgtg cgtaagatcc gttttgaagg taacgatacc tcgaaagatg ccgtcctgcg
    18961 tcgcgaaatg cgtcagatgg aaggtgcatg gctgggaagc gatctggtcg atcagggtaa
    19021 ggagcgtctg aatcgtctgg gcttctttga aactgtcgat accgataccc aacgtgttcc
    19081 gggtagcccg gaccaggtag atgtagtcta caaggtgaaa gaacgtaaca ccggtagctt
    19141 caacttcggg attggttacg gtactgaaag tggcgtgagc ttccaggctg gcgtgcaaca
    19201 ggataactgg ttaggtacag gttatgctgt tggtatcaac gggaccaaaa acgattacca
    19261 gacctatgct gaattgtcgg taaccaaccc gtacttcact gtagatggtg taagcctcgg
    19321 tggtcgtctc ttctataatg acttccaggc agatgacgcc gacctgtccg actataccaa
    19381 caagagttat ggtacagacg tgacgttggg cttcccgatc aacgaatata actcgctgcg
    19441 tgcaggtctg ggttatgtac ataactccct gtccaacatg cagcctcagg ttgcgatgtg
    19501 gcgttatctg tactctatgg gtgaacatcc gagcacctct gatcaggata acagcttcaa
    19561 aacggacgac ttcacgttca actatggttg gacctataac aagcttgacc gtggttactt
    19621 cccgacagat ggttcacgtg tcaacctgac cggtaaagtg accattcctg gatcggataa
    19681 cgaatactac aaagtgacgt tagacacggc gacttatgtg ccgatcgatg acgatcacaa
    19741 atgggttgtt ctggggcgta cccgctgggg ttatggtgat ggtttaggcg gcaaagagat
    19801 gccgttctac gagaacttct atgccggtgg ttccagcacc gtgcgtggct tccagtccaa
    19861 tacaattggt ccgaaagcag tttacttccc gcatcaggcc agtaattatg atccggacta
    19921 tgattacgaa tgtgcgactc aggacggcgc gaaagacctg tgtaaatcgg atgatgctgt
    19981 aggcggtaac gccatggcgg ttgccagcct cgagttcatc accccgacgc cgtttattag
    20041 cgataagtat gctaactcgg ttcgtacttc cttcttctgg gatatgggta ccgtttggga
    20101 tacaaactgg gattccagcc aatattctgg atatccggac tatagtgatc caagcaatat
    20161 ccgtatgtct gcgggtatcg cattacaatg gatgtcccca ttggggccgt tggtgttctc
    20221 ctacgcccag ccgttcaaaa agtacgatgg agacaaggca gaacagttcc agtttaacat
    20281 cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag cgatgacttt
    20341 aggcgatcaa tataagatcg ccgggccacg caaagaactg caccctccgg tgcaaatggg
    20401 atggtaagga gtttattgtg aaaaagtggt tattagcggc aggtctcggt ttagcactgg
    20461 caacttctgc tcaggcggct gacaaaattg caatcgtcaa tatgggcagc ctgttccagc
    20521 aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa ggccgtgcca
    20581 gcgaactgca gcgtatggaa accgatctgc aggctaaaat gaaaaagctg cagtccatga
    20641 aagcgggcag cgatcgcact aagctggaaa aagacgtgat ggctcagcgc cagacttttg
    20701 ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa gaacgcggca
    20761 aactggttac tcgtatccag actgctgtga aatccgttgc caacagccag gatatcgatc
    20821 tggttgttga tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac atcactgccg
    20881 acgtactgaa acaggttaaa taagtaatgc cttcaattcg actggctgat ttagcgcagc
    20941 agttggatgc agaactacac ggtgatggcg atatcgtcat caccggcgtt gcgtccatgc
    21001 aatctgcaca aacaggtcac attacgttca tggttaaccc aaaataccgt gagcatttag
    21061 gcttgtgcca ggcgtccgcg gttgtcatga cccaggacga tcttcctttc gcgaaaagtg
    21121 ccgcgctggt agtgaagaat ccctacctga cttacgcgcg catggcgcaa attttagata
    21181 ccacgccgca gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg acggcgaagc
    21241 tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga gtccggcgtt gaactgggcg
    21301 ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa cagcaaaatc ggtgcaggtt
    21361 cgcgtctctg ggcgaacgta accatttacc atgagatcca gatcggtcag aattgcctga
    21421 tccagtccgg aacagtggta ggcgcagacg gctttggtta tgccaacgat cgtggtaact
    21481 gggtgaagat cccacagatt ggtcgcgtaa ttattggcga tcgcgtggag atcggtgcct
    21541 gcacaaccat cgatcgcggc gcgctggatg acactgttat tggcaatggc gtgatcattg
    21601 ataaccagtg ccagattgca cataacgtcg tgattggcga caatacggcg gttgccggtg
    21661 gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg catgatcggc ggagccagcg
    21721 taatcaacgg gcatatggaa atatgcgaca aagtgacggt tacgggcatg ggtatggtga
    21781 tgcgtcccat cactgaacca ggcgtctatt cctcaggcat tccgctgcaa cccaacaaag
    21841 tctggcgcaa aaccgctgca ctggtgatga acattgatga catgagcaag cgtctgaaat
    21901 cgcttgagcg caaggttaat caacaagact aacgttccat cttttgttcg ccaaacttta
    21961 cggcctgtct cattcttacg attgcggcag gccgtgttat tattgtcgtt tcttatattt
    22021 tgacaggaag agtatcttga ctactaacac tcatactctg cagattgaag agattttaga
    22081 acttctgccg caccgtttcc cgttcttact ggtggatcgc gtgctggatt ttgaagaagg
    22141 tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc agggccattt
    22201 ccctggaaaa ccgattttcc cgggtgtgct gattctggaa gcaatggcac aggcaacagg
    22261 tattctggcg tttaaaagcg taggaaaact ggaaccgggt gagctgtact acttcgctgg
    22321 tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga tcatggaagt
    22381 cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc tggtcgatgg
    22441 taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct gatacgtgat
    22501 tgataaatcc gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt caattggcgc
    22561 gaacgcacac attggtcctt tttgtatcgt tggaccccat gtcgaaattg gtgagggtac
    22621 cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa attggccgcg ataatgagat
    22681 ttatcagttc gcctccatcg gcgaagttaa ccaggatctg aaatatgctg gcgaaccgac
    22741 ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc atcgtggcac
    22801 agtccagggc ggtggattga cgaaggtggg cagcgacaac ttactgatga tcaacgcgca
    22861 cattgcgcac gattgtacgg taggtaaccg ctgtattctc gccaacaacg caacgctggc
    22921 gggtcacgta tcggttgacg acttcgcgat catcggcggc atgaccgcag tccatcagtt
    22981 ctgcatcatt ggtgcgcacg tgatggttgg cggctgctcc ggtgtggcgc aggacgtccc
    23041 tccttatgtc attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata tcgaagggct
    23101 gaagcgccgc ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt ataagctgat
    23161 ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac tggcggaaac
    23221 atatccggaa gtgaaagcct ttaccgattt ctttgcacgc tcaacgcgcg gtctgattcg
    23281 ttaatgactg aacagcgtcc attaacgatt gccctggtcg ccggagaaac ctccggcgat
    23341 atcctggggg ccggtttaat ccgcgctctg aaagaacatg tgcccaacgc ccgctttgtt
    23401 ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag cttggtacga aatggaagaa
    23461 ctggcggtaa tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact gcatattcgt
    23521 gccgatctga caaagcgttt tggcgaactg aagccagatg tttttgttgg tattgatgcg
    23581 cccgacttca atattactct tgaaggtaac ctcaaaaagc agggtatcaa aaccattcat
    23641 tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg ttttcaaaat aggcagagcc
    23701 accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa atacaatgta
    23761 ccgtgccgct ttatcggtca taccatggct gatgccatgc cgttagatcc agataaaaat
    23821 gccgcccgtg atgtgctggg gatccctcac gatgcccact gcctggcgtt gctaccgggg
    23881 agccgtggtg cagaagttga aatgcttagt gccgatttcc tgaaaacggc ccagcttttg
    23941 cgccagacat atccggatct cgaaatcgtg gtgccactgg tgaatgccaa acgccgcgag
    24001 cagtttgaac gcatcaaagc tgaagtcgcg ccagaccttt cagttcattt gctggatggg
    24061 atgggccgtg aggcgatggt cgccagcgat gcggcgctac tggcgtcggg tacggcagcc
    24121 ctggagtgta tgctggcgaa atgcccgatg gtggtgggat atcgcatgaa gccttttacc
    24181 ttctggttgg cgaagcggct ggtgaaaact gattatgtct cgctgccaaa tctgctggcg
    24241 ggcagagagt tagttaaaga attattgcag gaagagtgtg agccgcaaaa actggctgcg
    24301 gcgctgttac cgctgttggc gaacgggaaa accagccacg cgatgcacga taccttccgt
    24361 gaactgcatc agcagatccg ctgcaatgcc gatgagcagg cggcacaagc cgttctggag
    24421 ttagcacaat gatcgaattt gtttatccgc acacgcagct ggttgcgggt gtggatgaag
    24481 tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt gacccggcgc
    24541 gcccgattgc cgggctgaat gattccaaaa agctgagcga aaaacgccgt ctggcgctct
    24601 atgaagagat caaagagaaa gcgttgagct ggagtctggg ccgcgcggaa ccccacgaaa
    24661 tcgacgagct gaacattctt catgcgacca tgctggcgat gcagcgtgcc gtcgctgggc
    24721 tgcatattgc gccggaatat gtgttgattg atggtaaccg ctgcccgaaa ttaccgatgc
    24781 ctgcgatggc tgtggtgaaa ggcgatagcc gcgtaccgga aatcagtgcc gcgtctatcc
    24841 tggcgaaagt gacgcgtgac gccgaaatgg cggcgctgga tattgttttc ccgcaatatg
    24901 gttttgccca acacaaaggg tacccaaccg cttttcatct ggaaaaactg gctgaacacg
    24961 gcgcgaccga acaccatcgg cgcagctttg ggcctgtcaa acgcgcactg ggacttgcgt
    25021 cctgattctt gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa ccacgtttcg
    25081 tacacctgcg ggtgcacagc gactactcga tgatcgatgg cctggccaaa accgcaccgt
    25141 tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc gatcaccgat ttcaccaacc
    25201 tttgtggtct ggtgaagttc tacggagcgg gacatggcgc agggattaag cctatcgtcg
    25261 gggcagattt taacgtccag tgcgacctgc tgggtgatga gttaacccac ctgacggtac
    25321 tggcggcgaa caataccggc tatcagaatc tgacgttgct gatctcaaaa gcgtatcagc
    25381 gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg gcttatcgaa ttaaacgaag
    25441 ggttgatcct tctttccggc ggacgcatgg gcgacgtcgg acgcagtctt ttgcgtggta
    25501 acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg gatcgctatt
    25561 ttcttgagct gatccgcacc ggcaggccgg acgaagaaag ctatctgcac gcggcggtgg
    25621 aactggcgga agcgcgcggt ttgcccgtcg tggcgaccaa cgacgtgcgc tttatcgaca
    25681 gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt accctcgacg
    25741 atcctaaacg cccgcgtaac tattcgccgc agcaatatat gcgtagcgaa gaggagatgt
    25801 gcgagctgtt tgccgacatc cccgaagctc ttgccaacac cgttgagatc gccaaacgtt
    25861 gtaacgtaac cgtgcgtctt ggtgaatact tcctgccgca gttcccgacc ggggacatga
    25921 gcaccgaaga ttatctggtc aagcgtgcaa aagagggcct ggaagagcgt ctggcctttt
    25981 tattccctga cgaggaagaa cgtgttaagc gccgcccgga atatgacgaa cgtctggaga
    26041 ctgaacttca ggttatcaac cagatgggct tcccgggcta cttcctcatc gttatggaat
    26101 ttatccagtg gtcgaaagat aacggcgtac cggtagggcc aggccgtggc tccggtgcgg
    26161 gttcactggt ggcctacgcg ctgaaaatca ccgacctcga tccgctggaa tttgacctgc
    26221 tgttcgaacg tttccttaac ccggaacgtg tctccatgcc tgacttcgac gttgacttct
    26281 gtatggagaa acgcgatcag gttatcgagc acgtagcgga catgtacggt cgtgatgcgg
    26341 tatcgcagat catcaccttc ggtacaatgg cggcgaaagc ggtgatccgc gacgtaggcc
    26401 gcgtgctggg gcatccgtac ggctttgtcg atcgtatctc gaaactgatc ccgcccgatc
    26461 cggggatgac gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa atctacgaag
    26521 cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg gtcacccgta
    26581 acgccggtaa gcacgccggt ggggtggtta tcgcgccgac caaaattacc gattttgcgc
    26641 cgctttactg cgatgaagag ggcaaacatc cggtcaccca gtttgataaa agcgacgttg
    26701 aatacgccgg actggtgaag ttcgacttcc ttggtttgcg tacgctcacc atcatcaact
    26761 gggcgctgga gatgatcaac aagcggcggg cgaagaatgg cgagccgccg ctggatatcg
    26821 ctgcgatccc gctggatgat aagaaaagct tcgacatgct gcaacgctcg gaaaccacgg
    26881 cggtattcca gcttgaatcg cgcggcatga aggacctgat caagcgtctg caacctgact
    26941 gcttcgaaga tatgatcgca ctggtggcac tgttccgccc cggcccgttg cagtcaggga
    27001 tggtggataa ctttatcgac cgtaaacatg gtcgcgaaga gatttcctat ccggacgtac
    27061 agtggcagca tgaaagcctg aaaccggtac tggagccaac ctacggcatc atcctgtatc
    27121 aggaacaggt catgcagatt gcgcaggtgc tttctggtta taccctcggt ggcgcggata
    27181 tgctgcgtcg tgcgatgggt aagaaaaagc cggaagagat ggctaagcag cgttctgtat
    27241 ttgctgaagg tgcagaaaag aacggaatca acgccgaact ggcgatgaaa atcttcgacc
    27301 tggtggagaa attcgcgggt tacggattta acaaatcgca ctctgcggcc tatgctttgg
    27361 tgtcatatca aacgttatgg ctgaaagcgc actatccggc ggagtttatg gcggcggtaa
    27421 tgaccgccga tatggacaac accgagaagg tggtgggcct ggtggatgag tgctggcgga
    27481 tggggctgaa aatcctgcca ccagatataa actccggtct ttaccatttc cacgtcaacg
    27541 acgacggcga aatcgtgtat ggtattggcg ccatcaaagg ggtaggtgaa ggtccgattg
    27601 aggccatcat cgaagcccgt aataaaggcg gctacttccg cgaactgttt gatctctgcg
    27661 cccgtaccga caccaaaaag ttaaaccggc gggtgctgga aaaactgatc atgtccgggg
    27721 cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa ctcgctgggt gatgcgttaa
    27781 aagcggcaga tcaacacgcg aaagcggaag ctatcggtca ggccgatatg ttcggcgtgc
    27841 tggccgaaga gccggaacaa attgaacaat cctacgccag ctgccaaccg tggccggagc
    27901 aggtggtgtt agatggggaa cgtgaaacgt taggtctgta ccttacggga caccctatca
    27961 accagtattt aaaagagatt gagcgttatg tcggaggcgt aaggctgaaa gacatgcacc
    28021 cgacagaacg tggtaaagtc atcacggctg cggggctcgt tgttgccgcg cgggttatgg
    28081 tcaccaagcg cggcaatcgt atcggtatct gcacgctgga tgaccgttcc gggcggctgg
    28141 aagtgatgtt gtttactgac gccctggata aataccagca attgctggaa aaagaccgca
    28201 tacttatcgt cagcggacag gtcagctttg atgacttcag cggtgggctt aaaatgaccg
    28261 ctcgcgaagt gatggatatt gacgaagccc gggaaaaata tgctcgcggg cttgctatct
    28321 cgctgacgga caggcaaatt gatgaccagc ttttaaaccg actccgtcag tctctggaag
    28381 cccaccgctc tgggacaatt ccagtacatc tctactatca gagggcggat gcacgcgcgc
    28441 ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga tcgtttatta aacgatctcc
    28501 gtggcctcat tggttcggag caggtggaac tggagtttga ctaatacagg aatactatga
    28561 gtctgaattt ccttgatttt gaacagccga ttgcagagct ggaagcgaaa atcgattctc
    28621 tgactgcggt tagccgtcag gatgagaaac tggatattaa catcgatgaa gaagtgcatc
    28681 gtctgcgtga aaaaagcgta gaactgacac gtaaaatctt cgccgatctc ggtgcatggc
    28741 agattgcgca actggcacgc catccacagc gtccttatac cctggattac gttcgcctgg
    28801 catttgatga atttgacgaa ctggctggcg accgcgcgta tgcagacgat aaagctatcg
    28861 tcggtggtat cgcccgtctt gacggtcgtc cggtgatgat cattggtcat caaaaaggtc
    28921 gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc agcgccagaa ggttaccgca
    28981 aagcactgcg tctgatgcaa atggctgaac gctttaagat gcctatcatc acctttatcg
    29041 acaccccggg ggcttatcct ggcgtgggcg cagaagagcg tggtcagtct gaagccattg
    29101 cacgcaacct gcgtgaaatg tctcgcctcg gcgtaccggt agtttgtacg gttatcggtg
    29161 aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga taaagtgaat atgctgcaat
    29221 acagcaccta ttccgttatc tcgccggaag gttgtgcgtc cattctgtgg aagagcgccg
    29281 acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc tccgcgtctg aaagagctga
    29341 aactgatcga ctccatcatc ccggaaccgc tgggcggcgc tcaccgtaac ccggaagcga
    29401 tggcggtatc gttgaaagcg caactgctgg cggatctggc cgatctcgat gtgttaagca
    29461 ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag ctacggttac gcgtaattcg
    29521 caaaagttct gaaaaagggt cacttcgg
//
LOCUS       DAESHI010000045        28896 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.045, whole genome shotgun sequence.
ACCESSION   DAESHI010000045 DAESHI010000000
VERSION     DAESHI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28896
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.045"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..1262)
                     /locus_tag="KW011_002066"
     CDS             complement(<1..1262)
                     /locus_tag="KW011_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HBH7660399.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRAFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFTV
                     AADATESCEDRVALTWNNLRKTLLVHQAS"
     gene            1753..2310
                     /gene="pinE"
                     /locus_tag="KW011_002067"
                     /pseudo
     CDS             1753..2310
                     /gene="pinE"
                     /locus_tag="KW011_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(2649..3497)
                     /locus_tag="KW011_002068"
     CDS             complement(2649..3497)
                     /locus_tag="KW011_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH7660400.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3706..4341
                     /gene="yfhb"
                     /locus_tag="KW011_002069"
     CDS             3706..4341
                     /gene="yfhb"
                     /locus_tag="KW011_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HBH7660401.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            4366..4902
                     /gene="tadA"
                     /locus_tag="KW011_002070"
     CDS             4366..4902
                     /gene="tadA"
                     /locus_tag="KW011_002070"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBH7660402.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQPSTD"
     gene            complement(4899..6455)
                     /gene="mltF"
                     /locus_tag="KW011_002071"
     CDS             complement(4899..6455)
                     /gene="mltF"
                     /locus_tag="KW011_002071"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HBH7660403.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDCTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            6713..10600
                     /gene="purL"
                     /locus_tag="KW011_002072"
                     /gene_synonym="purI"
     CDS             6713..10600
                     /gene="purL"
                     /locus_tag="KW011_002072"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBH7660404.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            11158..12585
                     /gene="qseE"
                     /locus_tag="KW011_002073"
     CDS             11158..12585
                     /gene="qseE"
                     /locus_tag="KW011_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HBH7660405.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            12750..13463
                     /gene="qseG"
                     /locus_tag="KW011_002074"
     CDS             12750..13463
                     /gene="qseG"
                     /locus_tag="KW011_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HBH7660406.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            13453..14787
                     /gene="glrR"
                     /locus_tag="KW011_002075"
     CDS             13453..14787
                     /gene="glrR"
                     /locus_tag="KW011_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HBH7660407.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            14848..15186
                     /gene="glnB"
                     /locus_tag="KW011_002076"
     CDS             14848..15186
                     /gene="glnB"
                     /locus_tag="KW011_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HBH7660408.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(15231..16421)
                     /gene="hmpA"
                     /locus_tag="KW011_002077"
     CDS             complement(15231..16421)
                     /gene="hmpA"
                     /locus_tag="KW011_002077"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HBH7660409.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            16749..18002
                     /gene="glyA"
                     /locus_tag="KW011_002078"
     CDS             16749..18002
                     /gene="glyA"
                     /locus_tag="KW011_002078"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBH7660410.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            complement(18200..19393)
                     /locus_tag="KW011_002079"
                     /pseudo
     CDS             complement(18200..19393)
                     /locus_tag="KW011_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            19511..22788
                     /locus_tag="KW011_002080"
                     /pseudo
     CDS             19511..22788
                     /locus_tag="KW011_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            22885..23868
                     /locus_tag="KW011_002081"
     CDS             22885..23868
                     /locus_tag="KW011_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBH7660411.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            23891..25402
                     /locus_tag="KW011_002082"
     CDS             23891..25402
                     /locus_tag="KW011_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBH7660412.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            25427..26425
                     /locus_tag="KW011_002083"
     CDS             25427..26425
                     /locus_tag="KW011_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH7660413.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            26458..27552
                     /locus_tag="KW011_002084"
     CDS             26458..27552
                     /locus_tag="KW011_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBH7660414.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHCATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            27564..28436
                     /locus_tag="KW011_002085"
     CDS             27564..28436
                     /locus_tag="KW011_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HBH7660415.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(28484..>28896)
                     /locus_tag="KW011_002086"
     CDS             complement(28484..>28896)
                     /locus_tag="KW011_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBH7660416.1"
                     /translation="RYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQYMAS
                     LGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTGDA
                     VGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
BASE COUNT         6867 a         7144 c         7865 g         7020 t
ORIGIN      
        1 gatgcctgat ggaccaggag ggttttccgg agattgttcc atgtgagcgc gacacggtcc
       61 tcacagctct cagtggcatc agcagcaaca gtgaaagact gctgtcgaag ctccgcagag
      121 gcactgagtt tttccagcca tgcagcgacc tgttcacgga atccggaggt attgcgtgca
      181 gagacggtat cggaaaggcg gtcaaggaac gcggagaatg cccgggaatt ctcctcggtg
      241 gaaaaagaag tccattgtgt agaaacctcc cgacgatctg cttcaggaaa ccaggctgcc
      301 acagtttcgg gaagaggggg gatctgaaag ttctgccccc cttcagtgat gactctgata
      361 tggcgcgaat gtaaattttg cggcaacatc tggcgtaaaa acagtctgtt ttggtctgac
      421 agggaaatac gaaactgagg cccgttaaaa tttggaaccg aagtttgctg tattaagaat
      481 agcagagttt gatctgataa ggggttattt gcaatctcaa tagttatatg aggatctaaa
      541 cgaaaaagat ttgtcgggat tgtagttatt tggttgtttt caaaataaaa aaatcttctc
      601 atgataatat tttcttggat gtttggtaag ttgcttaatc tattgctata tgcaaaaaga
      661 agttgcagtc tcctaggtaa agggggcagt tcagtaagat ggttgtgcgc aaccttaagt
      721 atcttaagat tagaaggcag agatggaaga gtttgcagtt cgttgttcct aacctcaagt
      781 ttttttaaat tcaagggtaa tgaaggcagt gtggataacc tattatactc tgcgcttagt
      841 gatttgatgg tgtctggtaa taacggcaga ttctctaatt gattttcgga cacatccaaa
      901 gttattagat aatgtggtaa tgcagatata tgtgttaaga aattatctct tgcaataagt
      961 tctttaatat tttcaggtaa atcagtaagg atggttaatt tattacagct cacatcaagc
     1021 tttttaatgc aagcagggag tacaggtaac gaggaaagcg ccaaggctcg caggtccagt
     1081 ttatgcccct tagaggtaag acatgcacga agtcttctta ccgcctcagt acgctgttct
     1141 tccggtaacc cctgtttctc ccaatcatcc cattcggaca aataatggtt gggggatgta
     1201 attgtgctga tatgtggaaa taaacaggat gaaatatttc tgagcatatt aatttctcgc
     1261 atatggatat ggaattagat tgtgaataaa ttgtatttaa tgtatctctc ctaatgagga
     1321 gggtgattca gaatattcgc cataagggaa tgagtgttat atcatgcttt ctaccctggt
     1381 aagtatattt gcttttttcg tatatttggt tcatcttttt ttgatgtttt acatattcac
     1441 aatgcagagg atttatacca gaagacatta gtcttgagta agatactaca acatctccaa
     1501 ctattttagc cagcctgact acttgacttg gtaatatttg ttgctaataa tcatgtataa
     1561 aataagctta tatacatagt aacttcagca aaggatttag taatgatcca aatcatatct
     1621 caatgtactg tttttttctt taaaaaaaca gtacattcta ctttataatg actgtgtatt
     1681 cccatggaag gagatcaccc aattctcccc cccgactttt gacgcagtgt cttgttaaca
     1741 gggggattta ccatgcttat tggctatgta cgcgtatcaa caaatgacca gaataccgat
     1801 ctacaacgta atgcgttgaa ctgtgcagga tgcgagctga tttttgaaga caagataagt
     1861 ggcacaaagt ccgaaaggcc ggggctgaaa aaactgctca ggacattatc ggcaggcgac
     1921 acactggttg tctggaagct ggatcggctg gggcgcagta tgcggcatct tgtcgtgctg
     1981 gtggaggagt tgcgcgaacg aggcatcaac tttcgtagtc tgactgattc aattgatacc
     2041 agcacaccaa tgggacgctt tttcttccat gtgatgggtg ccctggctga aatggaacgt
     2101 gaactgattg ttgaacgaac aaaagcagga ctggaagctg ctcgcgcaca gggacgaatt
     2161 ggcggtcgcc gtcccaaact ttcaccagaa taatgggcgc aggccgggcg actaattgca
     2221 tcaggagttt ctcgccagaa ggtggcgatc atctatgatg ttggcgaatc gacttttttg
     2281 tataagaggt ttccagccag ggttatataa aaccgcagac acggcgtata caagaacgtg
     2341 ctacggttga ctgaaaagtt aatgtggtat gtcacaggcc ttttgcgccg taaagccccg
     2401 tcctttaggg cgcggaggac gttaaagaaa gtggtgttct ttgtgttgct gaacttatgc
     2461 aaaatttttt tgcaccataa atccaacaac acattcaata acacattgct gtatcactat
     2521 gaaaataaat gtgtttttat ttgatttcac acatatcaca attgatgcat tttttagtga
     2581 ttaacaacgc cataaggaaa ctctcaaaac aacacaaacc gggcgggcat tgtacgcgca
     2641 ttctttactc agaccagttt tttcaccagc gcttcactat gacgaatacg ttctggtgcc
     2701 agttccagat cctgctgaat cagcgcaatg aacagcaaat ccgttaacat tccctgagcg
     2761 tgacaagcag agattgaagc actgtttgtc gcttgttctt cggcaatggt atacaggcaa
     2821 tgagaagcac gctgttgcag ggcattcgga gtaaagccgg taatcgccag cacttttccg
     2881 cccactcgca gcatctcatc tgccgccagg tttaactcgc gtcgtacacc ggtgtaggaa
     2941 atggctaata acagatcgtc aggggacgac gcctgtactg ttgcgagtag cgcatgcata
     3001 tcgcgcactg cggcagcatt gaagccaatc ttcatcagct tccaggcaaa gttttgcgcc
     3061 accagacccg aagcgccaat accggtcaga attatccgcc gcgcagagcg caacattgtt
     3121 acgcattcat gcagtttctc ttcactatta acgtttagcg ttgcgtacat agcggcagta
     3181 ttttctttaa tcagtttttc gccgaccagc cgtaacggat catcaccgcg gatttggtta
     3241 tgaatgggca cggagggcga ttccggctgg cttgccagcg cttcactcaa cgccaactta
     3301 agcgccggaa aacctttata gccgagtttt tgcgcgaact tcacgacgct ggactgactg
     3361 actccggctt cgttggccag ttgctgagag cttaaatggc gcgccgtatc aggttgtagc
     3421 agcagataat ccgccagttt tttatcactc tgggcaagcc cctggtaacg ctgacggata
     3481 cgaagtaagc cgttcatatc tttcctcgcg atgaaatagt ggcgacaaca ggcaatatga
     3541 gacacacgcg agtgaatttt atattccatt gccgctaatt gccatgaatt aaaaattcca
     3601 gttagccatg atgcggtcga ttgttgccgg aaaagtgatg atctatctgt taggctatgt
     3661 agtagcgaaa attaatcagg atgtttcagt ccagaggagt atggtttggc aactcacgag
     3721 cgtcgtgtgg tgttttttga cttagatgga acattgcatc agcaggatat gttcggcagt
     3781 tttctgcgct atttactgcg tcgccaaccg ctgaatgcgt tacttgtcct gccgttgtta
     3841 ccgattatag ccattgcgtt attgataaaa ggtcgtgcgg cacgctggcc gatgagtctg
     3901 cttctgtggg ggtgcacttt tggtcacagc gaagcacgtt tacagacgtt gcaggccgat
     3961 ttcgtgcgct ggtttcgcga caatgttacc gcctttccgc tggttcagga gcgattaacc
     4021 acctacctgt taagttccga tgctgatatc tggttgatta ccggctctcc gcagccgctg
     4081 gttgaagcgg tttatttcga tacgccctgg ctgccgcggg ttaatcttat cgccagccaa
     4141 attcagcgtg gctatggtgg ttgggtattg acgatgcgtt gtctgggaca tgaaaaggtc
     4201 gcacaactgg agcgcaaaat cggcactccg ctgcggctgt acagtggcta tagcgacagt
     4261 aatcaggaca atccgctgct ttatttctgt cagcatcgtt ggcgagtaac cccgcgcggt
     4321 gaactccagc aactggaata gagtaaagca tagcgtccgt gtataatgcg ccgcgctttt
     4381 ataaccggag ttttcttttt gtctgaagtc gaatttagcc acgaatactg gatgcgtcac
     4441 gcgctgacgc tggcgaaacg tgcctgggat gagcgggaag tgccggtcgg cgcggtatta
     4501 gtgcataaca atcgggtaat cggcgaaggc tggaaccgcc cgattggtcg ccatgatccc
     4561 accgcacatg cagaaatcat ggccctgcgg cagggtggtc tggtgatgca aaattatcgt
     4621 ctgatcgacg ccacgttgta tgtcacgctt gaaccatgtg taatgtgtgc cggagcgatg
     4681 atccacagtc gcattggtcg cgtggtcttt ggtgcgcgtg acgcgaaaac tggcgctgcg
     4741 ggatctttaa tggatgtgct gcatcatccg ggtatgaatc accgagtgga aattacggaa
     4801 ggaatactgg cggatgagtg cgcggcgttg ctcagtgact tctttcgcat gcgccgccag
     4861 gaaattaaag cgcagaaaaa agcgcaaccc tcgacggatt aattttgttt ctcttcactc
     4921 cctttcctgg aaaacagcag agagggagaa tgggtcaaat agtttgatga cgactgggaa
     4981 agaaacgaga gaaaaggaaa tttctctttg cccaactccg taggcgatac cgccggataa
     5041 tcctgcgcca gttgcatcgc cgcttctgta gcctgcttct ctttctcttg cagataaccc
     5101 accaggctaa tctgatactt acgaatattt tcgacataag cgtaggcttc atgtccacga
     5161 gcgtagccgt aagtcagctt gctgtaatag ggtttctggc taagtaaagg cagacgctgt
     5221 tttacgtcag cccaactgtc aggattccct ttggtttttg ccgtcagggc gcgggcatcc
     5281 agcatatgcg catagcccat attgtacgca gcgagggcaa accagatccg ctcgttctcc
     5341 ggcacacttt ccggcacttt actcatcata tcctgcaaat aacgcacgcc accgctgatg
     5401 ctctgttcgg catcggtacg atccgtaatg ccgaggcttt gcgcggtatt tttggttaac
     5461 atcatcatgc cgcgcacacc cgtcggtgaa gtggcctgtg catcccagtg cgattcctga
     5521 taagcaatag cggccagcaa acgccagtca atttcttcgg cgtatttctc aaacaggggc
     5581 tttaactgcg gcagtaccgc atcgacggcg cgtaaaaatg tgcgcgtatc gacgtaatca
     5641 aaatcatcgc catgccccag gtatttctct tcaatgcgtg ccagcgtaca gtcttcattc
     5701 atttcgttga agaagtcgag cagggcggcg gaaagggtat tatcgccatc taacgggcta
     5761 aaccaagtca ccggttgttc atcggtgata tcgagcgcta cggcgagctc cgggtgaacg
     5821 cgctgaaaca ggctgatggc gacagaatca gcaatggtgt aatcgagttt tccttcgatg
     5881 acatcttcca ttaattccgc agagcctttt ttgtcgtcta ccttccagct taattccggg
     5941 aattttgtgt ctttcagggt ttggagatcg ttaaccacca cgtgacccgg tgcaacggtg
     6001 agttgctccg ccgtcaggtt gcccagtgtg cgcgggcgat actgactcac tttataaacc
     6061 agttgttgtg acacggaata ataggtaggg ccaggctgat aattttttac ccgctcactg
     6121 ttatagacca gtcctgccgc cagcaggtcg gcgttaccat tatcgaggtc gtcaaacagc
     6181 tggctgatat tctgccgcac ggtcactttc agttttacac cgaggtaatc ggcaaactgt
     6241 ttcgccagtt cgtaatccag gccaaaaggt ttcccgttga tttcgttata agtcagggga
     6301 gtatgaatgg tgctcacacg caactctccc cgcgcttgaa tggcggcgat acggttgtcg
     6361 gctttaccaa accagggaat ggatggccag agagcgaccg cgagcagcag tgccagaatg
     6421 ccgatgaaca gataattaat ctttaatttt ttcaattagt taattctctg tgtcgtgcgc
     6481 gtcccagctt gaaaaaacgt aataatagtg aaaggtttac tcataaatga gcggcatttt
     6541 gcgtaaacct gcgccagatg gcaacttatt acagccattg gcggcacgcg ttgctaattc
     6601 acgatggtga ttttatttcc acgcaaacgg tttcgtcagc gcatcagatt ctttataatg
     6661 acgcccgttt cccccccttg ggtacaccga aagcttagaa gacgagagac ttatgatgga
     6721 aattctgcgt ggttcgcctg cactgtcggc attccgaatc aacaaactgc tggcacgttt
     6781 tcaggctgcc aggctcccgg ttcacaatat ttacgccgag tatgtccatt ttgctgacct
     6841 caatgcgccg ttaaacgatg atgagcacgc acaacttgaa cgcctgctga aatatggccc
     6901 ggcactcgcc agccacgccc cgcaaggcaa actcctgctg gtgaccccgc gtcctggcac
     6961 catctctccc tggtcttcga aagcgaccga tattgcccat aactgcgggc tacaacaggt
     7021 aaaccgcctt gagcgcggcg ttgcttacta tatagaagcc ggtacgctga ccaatgaaca
     7081 atggcagcag gttaccgctg aactgcacga ccgcatgatg gaaacggtct tttttgcttt
     7141 agatgatgca gagcagttgt ttgcccacca tcaaccgact ccggttacca gcgttgattt
     7201 gctggggcag ggccgtcagg cgctgatcga cgctaacctg cgtcttggcc tggctctggc
     7261 ggaagatgaa attgactatc tgcaggatgc tttcacaaag cttggtcgta acccgaacga
     7321 catcgagctg tatatgttcg ctcaggcgaa ctctgagcac tgccgccaca aaatttttaa
     7381 tgccgactgg attatcgacg gtgaacagca gccgaaatcg ctgttcaaga tgatcaaaaa
     7441 tactttcgaa accacgccag atcacgttct ctctgcttat aaagataacg ccgccgtaat
     7501 ggaaggttct gaagtgggcc gctactttgc tgaccacgaa acgggccgct acgatttcca
     7561 tcaggaaccg gcgcatattc tgatgaaagt cgaaactcac aaccacccga cggcgatttc
     7621 tccgtggccg ggcgcggcga cgggttccgg cggcgaaatt cgtgatgaag gcgccaccgg
     7681 acgcggtgcg aagccgaagg ctggtctggt tggtttctcc gtttccaacc tgcgaattcc
     7741 tggcttcgaa cagccgtggg aagaagattt cggtaagcct gagcgcattg tcaccgcgct
     7801 ggacatcatg accgaaggcc cgctgggcgg cgcggcgttt aacaacgaat ttggtcgtcc
     7861 ggcactgaat ggctacttcc gtacttatga agaaaaagtg aacagccaca acggcgaaga
     7921 gctgcgcggt tatcacaaac cgatcatgct ggcgggcggg atcggcaaca ttcgcgccga
     7981 tcacgtacaa aaaggcgaga tcaacgtcgg tgcgaagctg gtcgttctcg gcggcccggc
     8041 aatgaacatc ggtcttggcg gcggagcggc gtcttccatg gcgtcaggtc agtctgatgc
     8101 cgacctcgac tttgcttccg tacagcgcga caacccggag atggagcgtc gctgccagga
     8161 agtgatcgac cgttgctggc agcttggtga tgccaaccca atcctgttta tccacgacgt
     8221 cggcgctggc ggtctttcta acgcgatgcc ggaactggtg agcgacggcg ggcgcggcgg
     8281 taaatttgaa ctgcgcgata ttctaagcga cgaaccgggc atgagcccgc tggaaatctg
     8341 gtgtaacgaa tcccaggaac gctacgtgct ggcggttgct gccgatcaat taccgctgtt
     8401 tgacgaactg tgtaagcgtg agcgcgcacc ctacgcggtg attggtgaag cgaccgaaga
     8461 actgcatctt tctctgcacg atcgtcattt tgataatcag ccgatcgatc tgccgctgga
     8521 cgtcctgctt ggtaaaacgc cgaagatgac ccgcgatgta caaacgctga aagcgaaggg
     8581 cgatgcgctg gtccgtgaag ggatcaccat tgcagacgcg gtgaaacgcg tactgcatct
     8641 gccgaccgtg gcagagaaaa ccttcctggt gaccatcggc gaccgcagcg taaccggcat
     8701 ggtagcgcgc gatcagatgg tggggccgtg gcaggtgccg gtcgctaact gcgcggtcac
     8761 taccgccagc ctcgacagct actacggtga agcgatggcg attggcgagc gtgcgccggt
     8821 tgcactgctg gatttcgccg cctctgcccg tctggcggtc ggtgaagcgt taaccaatat
     8881 tgccgcaaca cagattggcg atatcaaacg cattaaactt tccgccaact ggatggcggc
     8941 ggcaggccac cctggtgaag atgcgggcct gtatgaagcc gttaaagccg tgggcgaaga
     9001 gctttgtccg gcgctgggcc tgacgatccc ggtgggtaaa gactctatgt cgatgaaaac
     9061 ccgctggcag gaaggtaacg aagagcgcga aatgacgtcg ccgctgtcgc tggtgatttc
     9121 tgcatttgcc cgcgtggaag atgtgcgtca caccatcacg ccacagcttt ctaccgaaga
     9181 taacacactg ctgctgattg atttgggcaa aggcaataac gcgctgggcg caacggcgct
     9241 ggcgcaggtt tatcgtcagc ttggcgacaa accggcagat gtacgcgacg tcgcgcaact
     9301 gaaaggcttc tatgacgcga ttcaggcgct ggttgcacag cgtaagctgc tcgcatacca
     9361 cgaccgctct gatggcggcc tgctggtaac gctggcggaa atggcctttg ctggtcattg
     9421 cggcattaac gcggatattg cctctctggg cgacgatcgc ctggcggcgt tgtttaacga
     9481 agaactgggt gcggtgattc aggttcgtgc cgctgaccgt gaagcggtcg agtccgtact
     9541 ggcacagcat gggcttgctg attgtgtcca ttatgtaggg caggcggttt ccggtgaccg
     9601 ttttgtgatt accgccaacg ggcagactgt attcagcgaa agccgcacca cgttgcgtgt
     9661 ctggtgggca gaaactacct ggcagatgca gcgcctgcgt gacaacccgg agtgtgctga
     9721 ccaggagcac caggcgaaat caaacgacgc cgatccgggc ctgaatgtga aactgtcgtt
     9781 cgatatcaac gaagatgtgg cagcaccgta tattgccact ggcgcacgtc cgaaagttgc
     9841 tgtgctgcgt gagcagggcg tgaactcgca tgttgaaatg gcggcagctt tccaccgtgc
     9901 aggctttgat gctatcgacg tgcatatgag tgacctgctg accggacgca cgggcctgga
     9961 agatttccac gccctggtcg cgtgcggtgg tttctcctac ggtgatgtgc tgggtgccgg
    10021 tgaaggttgg gcgaagtcaa tcctgttcaa tgaccgtgta cgcgatgagt ttgcaacctt
    10081 cttccaccgt ccgcaaacgc tggcgctggg ggtatgtaac ggttgccaga tgatgtctaa
    10141 cctgcgtgaa ctgatcccgg ggagcgagtt gtggccacgt tttgtgcgta acacctccga
    10201 tcgctttgaa gcgcgtttca gcctggttga agtaacccaa agcccgtctc tgctgttgca
    10261 ggggatggtg ggctcgcaaa tgccgattgc tgtctctcat ggtgaagggc gcgtggaagt
    10321 gcgtgatgcg gcgcatctgg cggcactgga aagcaaaggg ctggtggcac tgcgctatgt
    10381 cgataacttc ggcaaagtca ctgaaaccta cccggctaac ccgaacggtt caccgaacgg
    10441 tattacggcg gttacgactg aaagtggtcg agtcaccatt atgatgccgc acccggaacg
    10501 tgttttccgt actgtcagca actcctggca tccggaaaac tggggcgagg atggcccatg
    10561 gatgcgcatt ttccgcaatg cgcgtaagca gttggggtaa gtcatcagtt cattggtttt
    10621 gcagcccgga tgcggcatta agcaccaacc actatcataa gctcccaact ccgggagctt
    10681 ttttgtgtct gtaaatcacg acaatgggtg gtttaccgtg tcgctttctg gcgacactta
    10741 aatgattgat tttattgtaa tctaataagt ttatcttaag ttgtctcttt ttagcgacac
    10801 agtggctgat ttacattcaa atagcgaaga agccataaat ccctcaaagc aacgtataat
    10861 cagtacgtta ccaaactatt ttctttattg gcacagttac tgcataatag taagcagtgg
    10921 ctcattcacc gacttatgtc agccccttcg ggacgtgcta cataaaattc gaatgacgca
    10981 caacaaggtg cctgccgtcc aacttctgat atcagcgtag ctatatcaac catcgggcga
    11041 aacgtcgagt taggcaccgc cttattccat aacaaagccg ggtaattccc ggctttgttg
    11101 tatctgaact tcccctcggt tagcatcagg ctattcgcgt ctgacgagag taacaccttg
    11161 aaacgctggc ccgtttttcc ccgctcatta cgacaactgg taatgctggc atttttgcta
    11221 attctactac ccctgttggt gctggcatgg caagcctggc aaagcctgaa tgcgcttagc
    11281 gatcaggcgg cgctggttaa ccgcactacg cttattgatg cccggcgtag tgaagcgatg
    11341 accaacgcgg cgctggagat ggagcgtagc taccgtcagt attgcgtgct ggacgaccct
    11401 acgcttgcga aggtttatca aagccagcgc aagcgttaca gcgaaatgct cgatgcccac
    11461 gcaggcgtac tgccagacga taaactttac caggcattgc gtcaggattt gaacaatctg
    11521 gcccaacttc agtgtaacaa cagtggtccc gatgctgccg ccgccgcgcg tcttgaagcc
    11581 tttgccagtg ccaataccga aatggtgcag gccacgcgca cagtggtgtt ctctcgtggg
    11641 cagcaacttc agcgtgaaat cgccgaacgt gggcaatatt ttggttggca atcgctggtg
    11701 ctatttctgg tgagtctggt gatggtgcta cttttcacgc ggatgattat cgggccggtg
    11761 aaaaatatcg agcgcatgat caaccgtctg ggggaagggc gttctctggg caatagcgtt
    11821 tcgttcagtg gaccgagcga gttacgctcg gttgggcaac gtattctttg gttaagtgag
    11881 cgcctgtcat ggctggaatc ccaacgccat caatttttaa gacatttatc tcatgaatta
    11941 aaaacgccac tggcgagtat gcgcgagggc actgaattac tggctgacca ggttgtcggg
    12001 ccgcttacgc cagagcaaaa agaggtggtg agcattcttg atagcagcag ccgcaatttg
    12061 caaaaactga tcgaacaact gcttgattac aaccgtaaac aggcggacag tgcggtggaa
    12121 ctggagaatg ttgaattagc accgctggtg gagacagtgg tttctgctca tagcctgccc
    12181 gcacgggcta aaatgatgca taccgacgtc gatctcaaag caacagcttg cctggcggag
    12241 ccaatgctgc tgatgagcgt actggataat ctttactcca atgcggtgca ctacggggct
    12301 gaatccggta acatttgcct tcgcagcagt tcacatggtg cgcgggttta tattgatgtc
    12361 atcaatacag gcacgcccat tccgcaagag gaacgcgcca tgatcttcga accctttttt
    12421 cagggaagcc accagcgaaa aggggcggtg aagggcagcg gtctgggatt aagcattgcc
    12481 agggattgta ttcgccgtat gcaaggggaa ctgtatctgg tcgacgagag cgggcaagac
    12541 gtttgtttcc gcattgaatt accgtcgtcg aaaaacacga aataatgcta acagcctatc
    12601 ctatcaggct attttatttg tcagttcggc cccgggcagt gctcgaaatc ttcacgtacg
    12661 atatgtacgc tccggtttct ccgcgctgtc catgtccaga ctgtctgcaa caattacgcc
    12721 cactgggata ggctttaagt ctggtgaata tgcgacacat ttttcaacga ttattgcccc
    12781 gacggctgtg gctggcaggg ctgccatgtc tggcattgct gggttgcgtg cagaatcaca
    12841 ataagccagc cattgatacg ccagcagaag aaaaaattcc ggtttatcaa ctggctgact
    12901 atctttctac tgaatgtagc gacatctggg cgctgcaagg taaatcaacc gaaaccaatc
    12961 cgctttactg gctgcgggcg atggattgtg ctgatcgttt aatgcctgcg cagtcgcgtc
    13021 agcaagctcg ccaatatgac gacggcagct ggcaaaatac ctttaaacaa gggattttgc
    13081 tcgccgacgc caaaattacg ccgtatgagc gccgccagct ggtcgcgcgt attgaagcgt
    13141 taagtaccga gatcccggca caggttcgcc cactgtatca actctggcgc gacggtcagg
    13201 cgctgcaact gcaactggcg gaagagcgcc aacgttacag taaactccag cagtccagcg
    13261 acagcgagct ggatacattg cgccagcaac atcacgttct acagcagcaa ctggaactca
    13321 ccacccgcaa gctggaaaac ctgaccgata ttgaacgcca gctctcgacc cgcaaaccga
    13381 ctggaaattt ctcgccggat acgccgcacg agagtgaaaa gcccgcgcca tccacccatg
    13441 aggtcactcc tgatgagcca taaacctgcg catttattat tggtcgatga cgatccggga
    13501 ttgctgaaac tgcttggcct gcgcctgacc agcgaaggct acagtgtggt cacggcggaa
    13561 agtggcgctg aaggattacg ggtactgaat cgcgaaaaag tagatttagt tatcagcgac
    13621 ctgcggatgg atgaaatgga cggtatgcag ctatttgctg aaatccagaa agtgcagccg
    13681 ggaatgccag taattattct tactgcgcat ggttctatcc ccgatgccgt tgctgcaaca
    13741 cagcagggcg tttttagttt cctcaccaag cctgtcgata aagatgcgct atatcaggca
    13801 attgacgatg cgctggagca atccgcgcca gccaccgatg aacgctggcg cgaggcaatt
    13861 gtcacccgca gcccgctgat gctgcgtttg ctggaacagg cgcggctggt ggcgcaatca
    13921 gacgtcagcg ttttgattaa cggtcagagc ggcaccggga aagagatttt cgcccaggct
    13981 atccacaacg ccagcccgcg caacagcaaa ccatttattg ctattaactg tggcgcattg
    14041 cccgagcaat tgctggagtc ggagctgttt ggtcatgcgc gtggcgcgtt tactggcgct
    14101 gtcagcaatc gcgaaggttt attccaggcg gcggaaggcg gaacgctatt tctcgatgaa
    14161 attggcgata tgcctgcgcc gttacaggtc aaactgctgc gcgtgttgca ggagcgtaaa
    14221 gtgcgcccgc tgggcagtaa ccgcgatatt gatatcaatg tgcggattat ttctgccact
    14281 caccgtgatc tgccaaaagc gatggcgcgc ggggaattcc gcgaagatct ctattaccgc
    14341 ctcaacgttg tcagcctgaa aattccggca ctggcggagc gcacagaaga cattccgcta
    14401 ctggcaaatc acctgttgcg ccaggcggca gagcgacata aaccgtttgt ccgcgcgttc
    14461 tctaccgatg cgatgaaacg cctgatgacc gcgagctggc cgggtaatgt gcgccagttg
    14521 gtcaacgtga ttgaacagtg cgtggcgctg acctcatctc cggtgattag tgatgcgctg
    14581 gtggagcagg cgctggaggg tgaaaatacg gcgctgccaa cctttgttga ggcacgtaat
    14641 cagtttgaac tcaactattt gcgtaagctg ctgcaaatca ccaaaggcaa cgtcacccac
    14701 gcggcgagaa tggcggggcg caaccggaca gaattttata aactgctttc ccgacacgag
    14761 ctggatgcaa acgatttcaa ggaatgaatt ggcgttatgt gttacgttta gcagatcaaa
    14821 agacaggcga ccttttcaag gaatagcatg aaaaagattg atgcgattat aaaacccttc
    14881 aagctggacg atgtccgcga agcactggcc gaagtcggta ttaccggcat gacggtgacc
    14941 gaagtgaaag gctttggtcg ccagaaaggc cataccgagc tgtaccgcgg cgcggagtat
    15001 atggtggatt ttctgccgaa agtgaaaatt gagattgtcg taccggacga cattgtcgat
    15061 acctgtgtcg ataccattat tcgcacggcg caaaccggca aaatcggtga cggtaaaatc
    15121 ttcgtctttg acgtggcacg ggtcattcgc atccgtaccg gtgaagagga cgacgcggca
    15181 atttaaaccg cgtcaagggt tgccggatgt ttccatccgg caacatcaaa ttacagcacc
    15241 ttatgcgggc caaagcattc gtaatgaatg ttttcctgct tcacgcccag atccactaac
    15301 tgtttcgcgg taaactgcat gaagccaacc gggccgcaga gatagaactg cattgtcgga
    15361 tcgctgaacg caccttccag tttgctcaaa tccatcagac cttcgctatc aaactgacct
    15421 ttagcgcgat cggcttcgct cggctgacga tgccaggtgt gcgcggtaaa gcgcggcagt
    15481 gactgcccca gttccttaac ttcatcggca aaggcgtgaa catcgccatt ttctgccgca
    15541 tggaaccagt tcacttgtgc tgtgtggcct gcttttgcca gcgtgtcgag cattgccagc
    15601 attggcgttt gaccaacacc ggcagagatt aacgtcactg gtgtgtcatc tgcgacagcc
    15661 ataaagaaat cacctgccgg agcgaccagt ttcacgacat cgccaacatt ggcgtgattg
    15721 tgcaaccagt tggatacctg cccaccctct tcgcgtttca ccgcaatacg atagcctttg
    15781 ccatccggtt tgcgagtcaa agagtactga cgaatttcct gatgcgggaa accttccggc
    15841 ttcagccaga cgccgagata ttgccccgga cggtattctg ccactgcgcc accgtcgacc
    15901 ggctccagtt cgaagctggt gataagcgcg ctgcgcggtg ttttagccac aatgcggaaa
    15961 tcgcgagtac cttcccaacc accggctttg ctggcgtttt cgttatagat ttccgcctcg
    16021 cgattgataa atacattagc cagtacacca taggctttac cccacgcgtc cagcacttcc
    16081 tgccccgggc tgaacatttc gtccagcgtt gccaacaggt gttcaccgac gatgttgtac
    16141 tgttccggtt tgatctggaa gctggtgtgc ttctgcgcga ttttttctac cgctggcagc
    16201 agcgcaggca ggttttcaat attactggcg taggcggcaa tagcgttaaa cagggcttca
    16261 cgttgatcgc cattacgctg gttactcatg ttaaaaattt ctttgagttc tgggttatga
    16321 gtaaacatac ggtcgtagaa atgggcggtt aactttggcc ccgtttccac cagtaaaggg
    16381 atggtggctt ttactgtagc gatggtttga gcgtcaagca tatggtcttc ctttttttgc
    16441 atcttaattg atgtatctca aatgcatctt ataaaaaata gccctgcaat gtaaatggtt
    16501 ctttggtgtt tttcagaaag aatgtgatga agtgaaaaat ttgcatcaca aacctgaaaa
    16561 gaaatccgtt tccggttgca agctctttat tctccaaagc cttgcgtagc ctgaaggtaa
    16621 tcgtttgcgt aaattccttt gtcaagacct gttatcgcac aatgattcgg ttatactgtt
    16681 cgccgttgtc caacaggacc gcctataaag gccaaaaatt ttattgttag ctgagtcagg
    16741 agatgcggat gttaaagcgt gaaatgaaca ttgccgatta tgatgccgaa ctgtggcagg
    16801 ctatggagca ggaaaaagta cgtcaggaag agcacatcga actgatcgcc tccgaaaact
    16861 acaccagccc gcgcgtaatg caggcgcagg gttctcagct gaccaacaaa tatgctgaag
    16921 gttatccggg caaacgctac tacggcggtt gcgagtatgt tgatatcgtt gaacaactgg
    16981 cgatcgatcg tgcgaaagaa ctgttcggcg ctgactacgc taacgtccag ccgcactccg
    17041 gctcccaggc taactttgcg gtctacaccg cgctgctgga accaggtgat accgttctgg
    17101 gtatgaacct ggcgcatggc ggtcacctga ctcacggttc tccggttaac ttctccggta
    17161 aactgtacaa catcgttcct tacggtatcg atgctaccgg tcatatcgac tacgccgatc
    17221 tggaaaaaca agccaaagaa cacaagccga aaatgattat cggtggtttc tctgcatatt
    17281 ccggcgtggt tgactgggcg aaaatgcgtg aaatcgctga cagcatcggt gcttacctgt
    17341 tcgttgatat ggcgcacgtt gcgggcctgg ttgctgctgg cgtctacccg aacccggttc
    17401 ctcatgctca cgttgttact accaccactc acaaaaccct ggcgggtccg cgcggcggcc
    17461 tgatcctggc gaaaggtggt agcgaagagc tgtacaaaaa actgaactct gccgttttcc
    17521 ctggtggtca gggcggtccg ttgatgcacg taatcgccgg taaagcggtt gctctgaaag
    17581 aagcgatgga gcctgagttc aaaacttacc agcagcaggt cgcgaaaaac gctaaagcga
    17641 tggtagaagt gttccttgag cgcggctaca aagtggtttc cggcggcact gataaccacc
    17701 tgttcctggt tgatctggtt gataaaaacc tgaccggtaa agaagcagac gccgctctgg
    17761 gccgtgctaa catcaccgtc aacaaaaaca gcgtaccgaa cgatccgaag agcccgtttg
    17821 tgacctccgg tattcgcgtg ggtactccgg cgattacgcg tcgcggcttc aaagaagcag
    17881 aagcgaaaga actggctggc tggatgtgtg acgtgctgga cagcatcaat gatgaagccg
    17941 ttatcgagcg catcaaaggt aaagttctcg acatctgcgc acgtttcccg gtttacgcat
    18001 aagcgaaacg gtgatttgct gtcaatgtgc tcgttgttca tgccggatgc ggcgtgaacg
    18061 ccttatccgg cctacaaaac tttgcaaatt caatatattg caatatccgt gtaggcctga
    18121 taagcgtagc gcatcaggca atcttaggta tgtgatcatc aaggcttcct tcgggaagcc
    18181 tttctacgtt attgcgccat caaatctgtc gtaactgcgc ctcaacatac aaatagccaa
    18241 tccccaacac ctgttgtgcg cggcttaatt gcccaaagcc aatttgcgtt gctttcacgc
    18301 tcggcccttc gtcgcggtcg aacgggttaa atcccgtctg gcgaataatg gtattaagcc
    18361 agtgctcacc aaaggcacaa ctgcgaccgt acaaccaaat ctgattaata ttgaggatgt
    18421 tgaggaagtt atacagactc aaaccaatgg cattggcact gcgatcaacc caactggtga
    18481 tccacggttc tccactctac caggcagcga ttaactgcgc tgtagtcagt ttttccggat
    18541 caagctgagt attaaccggt tgtgatttta gccatacccg cgcctgtttt tttaatgcac
    18601 ttaacgaggc gacggtttcc aggcagccgt aacgtccaca gtcgcagacg acgccatccg
    18661 gattaacgat ggtgtgacca atctgtccgc taccatacaa actgccgcga taaatttgct
    18721 cgttaatcac gaacgacgag ccaatgccgt aatcaacgtt gatcacgcag aaatcccgtt
    18781 tctgcgaatt attttgccat ttctccgcca gcgccagcat cacgcagtca ttatcgacca
    18841 tcacccgaat gccgagcttc tcttccagca gatacttcac ctcaaccggc gttgtccacg
    18901 gcgcttgcgg catggtttgc gacacgccgg tcaccggatc aacctgaccg tggattgcca
    18961 gcgccagatt gatggtgcgg tccggccaca atttacggtg gcgatgccag catttttcga
    19021 tttcggacag cagcgcctgc ggcgtcggtg catcaatctg taaatactca aattcacctt
    19081 tcggacttaa acaagcatta gcaacctgac actcaatact ggtgggcgtc acgttcaggc
    19141 acagcgtcca gtcaccttcc ggcgcaatca gccatgtacc gctactatgc ccgcgcgtct
    19201 ggctttcatc gtcgatattc accacccgtt tttcgctttc cagttcctgc aaaatattac
    19261 tgactgccgg aatcgaaatt tgcgccagcc gggccagcgt tgatttattg gcgcgctttt
    19321 gccggtacag cagttccaga atcacgcatt tgttatggtg gcgaatctgt tgattgttaa
    19381 tgcaggctct catcttcact aaaccttgtt tagttaattg cgtgattatt ccgcgaaaac
    19441 attgccagat tgttcaatac actgccacaa atcttttaat tcagtatgtc ttgttaatat
    19501 tgagggcacc atgactccag taaaagtgtg gcaagagcgc gttgagatcc cgacctatga
    19561 aaccgggccg caggatatac atcccatgtt cctggaaaat cgcgtttatc agggatcgtc
    19621 cggcgcggtt tatccctacg gcgtgaccga tacgctgagc gagcagaaaa ccctgaaatc
    19681 ctggcaggcg gtgtggctgg aaaacgacta catcaaagtg atgatcctgc cggaactggg
    19741 cggtcgggtg catcgcgcgt gggataaagt gaaacagcgc gattttgttt atcacaatga
    19801 agtcattaaa cctgcgctgg tggggctgct ggggccgtgg atttccggtg ggattgagtt
    19861 taactggccg caacatcatc gcccgaccac ctttatgccc gttgatttca ccctcgaagc
    19921 ccatgaagac ggtgcacaga cggtgtgggt aggcgaaacg gagccgatgc atggtttaca
    19981 ggtgatgaca ggtttcaccc tgcgccctgg gcggcgctgg aaatcgccag ccgcgtctat
    20041 aacggcaacg ccacgccgcg tcatttcttg tggtgggcca acccggcagt gaaagggggt
    20101 gaagggcatc agagcgtttt cccaccggat ttgactgcgg tgtttgatca cggcaaacgg
    20161 gccgtctccg ctttccccat cgctaccggc acttactaca aagtggacta ctccgctgga
    20221 gtggacattt ctcgctataa aaatgtgccc gttccaacct catatatggc cgaaaaatcg
    20281 cagtacgatt ttgtcggcgc gtggtgtcac gatgaagatg gcggtttgct gcacgttgcc
    20341 aaccaccata ttgcgccagg taaaaaacag tggagttggg gacacagtga atttggccag
    20401 gcgtgggata agagtctgac cgacaataac ggcccgtata tcgaactgat gaccggtatt
    20461 tttgccgata accagcccga ttttacctgg cttgatgctt acgaagagaa gcgttttgag
    20521 cagtatttcc tgccttatca ttctctgggc atggtgcaaa atgcctcccg cgatgcggtg
    20581 ataaaactcc agcgtagtga gcaggggatt gagtgggggc tgtatgccat ctctccgttg
    20641 aacggatacc gcctggcgat ccacgaaatc ggcaaatgca acgcgttact tgatgatgcc
    20701 gtggcactga tgcctgcgac cgccatccag ggcgtgttgc acggtatcaa tcctgatagg
    20761 ctgaccattg agctttccga tgccgacggc aatattgtac tgagttatca ggaacatcag
    20821 ccgcaagcgt tgccgttacc ggacgtcgcc aaagcgccac tggcagcaca agacattacc
    20881 agtacagatg aagcctggtt tatcggtcag catctggagc aatatcatca cgccagccgt
    20941 tcaccgttcg attactacct gcgcggcgtg gcgctggacc cgctggatta tcgctgtaac
    21001 ctggcgctgg cgatgctgga atataaccgc gcagatttcc cgcaagcggt ggcgtatgcc
    21061 agtcaggcgc tgaaacgcgc acatgcgctg aacaaaaacc cgcagtgtgg acaagcaagt
    21121 ttgattcgcg ccagtgctta cgaacgtcag ggacaatatc aacaagccga agaggatttc
    21181 tggcgtgcgg tctggagcgg caacagcaaa gccggtggct attatggcct ggcacgactg
    21241 gcggcgcgta atggtaactt cgacgctggt ctggattttt gccaacaaag tcttcgcgcc
    21301 tgcccaacca atcaggaagt gctttgcctg cataatctgc tgctggtgtt aagtggtcgt
    21361 caggacaacg cgcgtttgca gcgcgcgaaa ctgctgcgtg attatccgct gaacgccatt
    21421 ctgtggtggc tgaactggtt cgatggtcgt agcgaatcag ccctcgcgca gtggcgcggt
    21481 ctgtgtcagg gacgcgacgt taacgccctg atgaccgccg ggcaactgat taactgggga
    21541 atgcccactc tggcgacaga gatgctgaac gcactggact gtcagcgcac gttatcgctt
    21601 tatctacaag ccagcttgct gccgaaagcc gaacgcggcg aactggtcgc aaaagccatt
    21661 gatgtcttcc cgcagtttgt ccgtttcccg aatacgctgg aagaagtggc ggcgctggag
    21721 agtattgaag agtgctggtt tgcccgtcat ttactggcct gcttctacta caacaagcgc
    21781 agctacaaca aagccattac tttatggcaa cgttgcgtag agatgtcgcc ggagtttgcc
    21841 gatggctggc gcgggttggc gatccatgcg tggaataagc aacacgatta tgaactggcc
    21901 gcgcgttatc ttgataatgc ttatcagctt gcgccgcagg atgcacgtct gcttttcgaa
    21961 cgggatttgc tggataagtt aagtggagcc acaccggaga aacgactggc gcgtctggaa
    22021 aataatctgg aaattgcgct gaaacgcgac gacatgaccg cagaactgct caatttgtgg
    22081 catctcacgg gtcaggcaga caaagcggcg gacattctcg ccacgcgcaa attccacccg
    22141 tgggaaggcg gggaagggaa ggtcaccagt cagtttatcc tcaaccagtt attacgcgcc
    22201 tggcagcatc ttgatgccag acagccgcag caggcctgcg aactgcttca tgccgcgctg
    22261 cattatccgg agaatttaag cgaaggccgt ttaccggggc aaactgataa cgacatctgg
    22321 ttctggcagg cgatatgcgc caacgcgcag ggcgatgaaa ctgaagcgac gcgttgttta
    22381 cgtctggcgg cgaccggcga tcgcaccatt aacatccaca gttattacaa cgatcagccg
    22441 gttgattatc tcttctggca aggaatggcg ctgcgactgc tgggcgaaca acaaatcgca
    22501 cagcaactgt ttagtgaaat gaaacagtgg gcgcaagaga tggcgaaaac cagtattgag
    22561 gcggatttct ttgctgtttc acaacctgac ctgttgtcgc tgtatggcga tttacaacag
    22621 cagcataaag aaaaatgcct gatggtggcg atgctggcgt ccgcgggact cggggaggtt
    22681 gcgcaatatg aatctgctcg cgctgaattg acggcgatta atccggcctg gccgaaagcg
    22741 gcattattca ccaccgtgat gccttttatt tttaactacg ttcactaaac cctattaact
    22801 gtaatatttg agcggcacgc gctgccgctc atcacaaaat aattagaata agccgttgtt
    22861 aataaaaact ctacaggagg cactatgcct aaaaaaatga gaacaacccg taatctatta
    22921 ttaatggcta cgctattagg tagcgcacta tttgccaggg ctgcggataa agaaatgacc
    22981 attggtgcaa tataccttga taccctggga tattacgctg gagtgcgtca gggcgttcag
    23041 gatgcggcaa aagattcttc agtacaggta cagttaattg aaactaacgc ccagggtgat
    23101 atttcgaaag aaagtacctt tgttgatacc ctcgtggcgc gtaatgtcga tgccattatt
    23161 ttatcggcag tgtctgaaaa tggcagtagc cgtaccgttc gtcgcgccag tgaagcgggt
    23221 attccggtga tttgctacaa cacctgtatt aatcaaaagg gtgtcgataa atatgtctcg
    23281 gcgtatctgg tcggcgatcc actggaattt ggtaaaaaac tgggtaacgc tgccgccgat
    23341 tattttattg ccaataaaat tgaccagccg aaaattgccg tcatcaattg cgaagccttt
    23401 gaagtttgtg tgcagcgacg taaaggattt gaagaagtat taaaatcccg cgttcccggc
    23461 gcgcaaattg tcgctaatca ggaagggact gttttaaata aagcgatttc cgttggtgaa
    23521 aaactgatta tctccacgcc ggatctcaac gccattatgg gggagtcggg cggtgcgaca
    23581 ctcggcgcgg taaaagcggt acgtaatcaa aatcaggccg gaaaaattgc tgttttcggt
    23641 tcggatatga caaccgaaat tgctcaggag ctggaaaaca atcaggtgct gaaagcggta
    23701 gtggatattt ccggtaagaa aatgggcaat gctgttttcg cgcaaacatt gaaggttatc
    23761 aataaacaag ccgacggtga aaaagtgatt caggtgccaa tcgatctcta taccaaaacg
    23821 gaagatggta aacagtggct ggcaacgcac gttgatggtc tgccctaatc gctgattcgc
    23881 agaggtccct atgttcacgg caacagaggc agtcccggta gcaaaagtgg tggcaggaaa
    23941 taagcgttac cccggcgtcg ttgcgctgga taacgttaac ttcacgctca ataaaggcga
    24001 agttcgtgcg ctgttaggta aaaacggcgc gggcaaatcg actctcattc gaatgcttac
    24061 cggcagcgaa cgcccggata gcggtgatat ctggattggc gagacgcgac tggaaggtga
    24121 cgaagctacg ttgactcgcc gtgccgctga actgggggtt cgtgcggttt atcaggaatt
    24181 aagtctggtg gaagggctga cggtggcaga aaacctctgc cttggtcaat ggcctcgccg
    24241 caacggcatg attgattacc tgcaaatggc gcaggatgcc caacgttgct tacaggcgct
    24301 gggcgttgac gttagtcctg aacaacttgt ttcaacgcta agcccggcgc aaaagcaact
    24361 ggtggaaatt gcacgggtga tgaagggcga gccgcgcgtg gtcattcttg atgaacccac
    24421 cagctcgctt gccagtgcag aagttgaact ggtgatcagt gcggtgaaaa agatgtcggc
    24481 actgggcgtg gcggtgattt atgtcagcca ccggatggaa gaaatccgcc gcattgcctc
    24541 ctgtgccacc gttatgcgcg atggtcaggt ggcgggcgat gtgatgctcg aaaacacctc
    24601 cacgcatcat attgtgtcgc taatgctagg gcgtgatcac gttgatattg cgccggttgc
    24661 ccctcaggaa attgtggatc aggccgtact ggaagtccgt gcgttacgcc ataagcccaa
    24721 gctggaggat atcagcttta cgttacgtcg cggcgaagtg cttggcattg ctggcctgct
    24781 gggggcaggg cgcagtgaat tgctgaaggc gattgttggg ctggagacgt atgaacaggg
    24841 cgaaattgtt atcaacggcg agaaaatcac gcgccccgat tacggcgaca tgctgaaacg
    24901 cggcattggc tatacgccag aaaaccgcaa agaagcgggg atcattccct ggttgggcgt
    24961 tgacgaaaat acagtgctga ccaatcggca aaaaatcagc gccaacggag tgctgcaatg
    25021 gtccaccatc cgccgcctga ccgaagaggt gatgcagcgg atgacggtca aggccgctag
    25081 tagcgaaacg cccatcggca cgctttctgg tggcaatcag caaaaagtgg tgatcggtcg
    25141 ttgggtctat gccgccagcc agattttgtt gctcgacgag ccaacgcgcg gcgtcgatat
    25201 cgaagccaaa cagcagattt accgtattgt ccgtgagctg gctgccgaag gaaaaagcgt
    25261 ggtgtttatc tccagtgaag tggaggagtt gccgctggtg tgtgaccgca tcctgttatt
    25321 acagcacggc acgttctcgc aggagtttca ctcgccggtc aatgtggatg agctgatgtc
    25381 cgccattctg tctgtgcact gatgaatttt accgaggaac ctgaagatgt ctgcttcgtc
    25441 attaccattg ccgcagggca agagcgtctc gctcaaacaa tttgtcagtc gccatattaa
    25501 tgagatcggt ttgctggtgg tgattgccat tctttatctg gtcttctccc tgaacgcgcc
    25561 tggctttatc tcattgaata accagatgaa cgtgctgcgc gatgccgcca ccattgggat
    25621 tgccgcctgg gcgatgacgc tgattattat ctccggtgaa attgatgtca gcgttgggcc
    25681 gatggtggct tttgtctcgg tgtgcctggc atttttgctg caatttgaag ttccgttggc
    25741 gattgcttgt ctgctggtgt tgctgttagg cgcgctgatg ggaacgctcg ccggggtgct
    25801 gcgcggcgtg tttaacgtac caagtttcgt tgccacactg gggctgtgga gcgccctgcg
    25861 cggaatgggg ctgtttatga ccaacgcact gccagtgccg attgacgaaa acgaggtgct
    25921 ggactggctg ggcggacaat ttctcggtgt gccagtatcc gcgctgatca tgatggtgtt
    25981 gtttgcgctg tttgtgttca ttagccgaaa aaccgccttc gggcgctcgg tttttgccgt
    26041 tggcggtaat gccacggcgg cgcagttgtg cggtattaac gttcgtcggg tgcgcattct
    26101 tatctttacc ctttcgggat tattagcggc ggtgaccggc attttgctgg cggcacgcct
    26161 cggttccggt aacgcaggtg ccgcaaacgg tctggagttt gacgtcatcg ccgcggtcgt
    26221 ggtcggcggt acggcacttt ccggcggtcg cggttccctg ttcggtacat tgcttggtgt
    26281 gctggtgatt acgctaatcg gtaacggtct ggtgctgctc ggtattaact cctttttcca
    26341 gcaggtggta cgcggcgtca tcatcgtggt ggcggtgctg gcgaatatct tgctgaccca
    26401 gcgaagcagt aaagcgaaac gctaaacctt aacgcccctg gcgcgggtgc ggcagggatg
    26461 aaaattaatc gtattgttaa tgagggattt atgaaaacga tgctggcagc ttatttacca
    26521 ggaaattcaa ccgtcgatct gcgggaagtt gcggtgccga cgccggggat taaccaggta
    26581 ctgatcaaaa tgaaatcctc cgggatttgc ggaagcgatg tccactatat ctatcatcaa
    26641 cactgtgcca cggcggcggc acccgataaa ccgttatacc agggatttat caacggtcat
    26701 gaaccgtgcg ggcagattgt ggcgatgggg caaggctgcc gccattttaa agagggcgac
    26761 cgcgtgctgg tgtatcacat ttctggctgt ggtttttgcc cgaactgccg ccgcggcttt
    26821 cctatctctt gtactggcaa aggaaaagcg gcttacggct ggcagcgtga cggcggtcat
    26881 gccgaatatc tgctggcgga agaaaaagat ctgatcctcc tgccggatgc gctgagctac
    26941 gaagatggtg cgtttatcag ttgcggcgtt ggcacggcct atgaagggat tttgcgcggc
    27001 gaagtttccg gcagcgataa cgtgctggtg gtcggtctgg ggccggtcgg catgatggcg
    27061 atgatgctgg cgaaaggtcg cggtgcaaaa cggatcatcg gcgttgatat gctgccggaa
    27121 cgtctggcga tggcaaaaca gttaggggtg atggatcacg gctatttagc aaccaccgaa
    27181 ggtctgccgc agattatcgc cgaactcacc cacggtggcg cggatgttgc gctcgattgt
    27241 tctggtaatg ccgcaggtcg cttgctggca ctgcaatcca ccgctgactg gggacgggtg
    27301 gtttacattg gtgaaaccgg aaaagtggaa ttcgaggtca gcgccgatct gatgcaccat
    27361 caacggcgga ttatcggctc ctgggtgacc agtctgttcc atatggaaaa atgcgcccac
    27421 gatttgactg actggaagct gtggccgcgt aacgccatta cccatcgctt ctcgctggaa
    27481 caggcaggtg atgcctatgc gctgatggcg agcggcaaat gcgggaaagt tgtgattaac
    27541 ttcccggatt aaggagttag cggatgacca tctatacctt atcccacggg tcgctaaagc
    27601 tggacgtttc tgaccagggc ggcgtaatcg aaggcttctg gcgcgatacg acgccgctct
    27661 tacgtcctgg taaaaaaagc ggtgtggcaa ccgatgcctc atgttttccg ctggtgccat
    27721 ttgcaaaccg ggtgagcggc aaccgttttg tctggcaggg gcgtgagtat caactgcaac
    27781 cgaatgttga gtgggatgcg cactatctac acggcgatgg ctggctgggc gagtggcaat
    27841 gtgtctcgca cagcgatgat agtttgtgtc tggtgtatga acatcgcagc ggtgtctatc
    27901 actatcgggt aagtcaggcg tttcatttaa cggcggatac gctgacggtg acgctctctg
    27961 tcaccaatca aggggcagag acgctgccat ttggtaccgg ctggcatcct tattttccgt
    28021 tgtcaccaca aacgcggatt caggcgcagg cgagcggtta ctggctggaa cgggagcagt
    28081 ggctggcggg tgagttttgc gagcagctac cgcaggaact ggattttaac cagctcgcgc
    28141 cgttgccgcg ccagtgggtg aacaatggtt ttgccggatg gaatggtcag gcccgcatcg
    28201 agcagccgca ggaaggttat gccatcatca tggaaacgac gccacctgca ccgtgttatt
    28261 tcatctttgt ttccgaccct gcgtttgata aaggatatgc gtttgatttc ttctgtctgg
    28321 aaccgatgag ccatgcgccg gatgatcatc atcgcccgga agggggtgat ctcattgcgc
    28381 tagcaccagg ggaatcaaca acttcagaga tgtcgttgcg ggtagagtgg ctgtaattta
    28441 ttaaatgccg gataaggcgt tcacgccgca tccggcatct ttcctaacgc cgatcgagag
    28501 aaattgcccc cggcccggta attgccaata gcaagaacgc gccagcgata ctgacattct
    28561 tccagaaatt aatcatattt ggcccaaccg catcgccggt catatcccag taatggtgac
    28621 caatcaccgc cgtacccagc gtgtagaaaa taaacagcac cgccagcgga cgggtgaaaa
    28681 agccaagcac gattaatatc gcggcgggca cttccataac taccgcaata atcgctgcca
    28741 gcatcggcat tggcgcgccc aacgaggcca tatattggac cgtaccgtca aagcccatca
    28801 ttttgggaaa accaaaaata atgaaaatta agaccacggc gatacgggca attaataaca
    28861 aaacggggcg ggcagctcca aaatcaaaat aacgta
//
LOCUS       DAESHI010000046        28457 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.046, whole genome shotgun sequence.
ACCESSION   DAESHI010000046 DAESHI010000000
VERSION     DAESHI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.046"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..216)
                     /locus_tag="KW011_002087"
     CDS             complement(<1..216)
                     /locus_tag="KW011_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH7660417.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLK"
     gene            224..655
                     /gene="tnpA"
                     /locus_tag="KW011_002088"
                     /pseudo
     CDS             224..655
                     /gene="tnpA"
                     /locus_tag="KW011_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(786..2109)
                     /locus_tag="KW011_002089"
                     /pseudo
     CDS             complement(786..2109)
                     /locus_tag="KW011_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(2109..3725)
                     /locus_tag="KW011_002090"
     CDS             complement(2109..3725)
                     /locus_tag="KW011_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH7660418.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            4021..4737
                     /locus_tag="KW011_002091"
     CDS             4021..4737
                     /locus_tag="KW011_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH7660419.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4734..6395)
                     /locus_tag="KW011_002092"
     CDS             complement(4734..6395)
                     /locus_tag="KW011_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HBH7660420.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6591..7019)
                     /gene="ibpB"
                     /locus_tag="KW011_002093"
     CDS             complement(6591..7019)
                     /gene="ibpB"
                     /locus_tag="KW011_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HBH7660421.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(7131..7544)
                     /gene="ibpA"
                     /locus_tag="KW011_002094"
     CDS             complement(7131..7544)
                     /gene="ibpA"
                     /locus_tag="KW011_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HBH7660422.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7538..7610)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7850..8182
                     /locus_tag="KW011_002095"
     CDS             7850..8182
                     /locus_tag="KW011_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBH7660423.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(8184..9398)
                     /locus_tag="KW011_002096"
     CDS             complement(8184..9398)
                     /locus_tag="KW011_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HBH7660424.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            9499..10563
                     /gene="cbrA"
                     /locus_tag="KW011_002097"
     CDS             9499..10563
                     /gene="cbrA"
                     /locus_tag="KW011_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HBH7660425.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10560..11897)
                     /locus_tag="KW011_002098"
     CDS             complement(10560..11897)
                     /locus_tag="KW011_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH7660426.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11972..13120)
                     /gene="dgoD"
                     /locus_tag="KW011_002099"
                     /pseudo
     CDS             complement(11972..13120)
                     /gene="dgoD"
                     /locus_tag="KW011_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(13117..13734)
                     /gene="dgoA"
                     /locus_tag="KW011_002100"
     CDS             complement(13117..13734)
                     /gene="dgoA"
                     /locus_tag="KW011_002100"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HBH7660427.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13718..14596)
                     /gene="dgoK"
                     /locus_tag="KW011_002101"
     CDS             complement(13718..14596)
                     /gene="dgoK"
                     /locus_tag="KW011_002101"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HBH7660428.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14593..15282)
                     /gene="dgoR"
                     /locus_tag="KW011_002102"
     CDS             complement(14593..15282)
                     /gene="dgoR"
                     /locus_tag="KW011_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HBH7660429.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            15560..16216
                     /locus_tag="KW011_002103"
     CDS             15560..16216
                     /locus_tag="KW011_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660430.1"
                     /translation="MKLNLKGFFKTAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLKDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYNWSTEEEKARVTQYRFACGVQHCTRTVENLEGTIHEKNKN
                     MDYSNVMAFYHPFKVRFYEYHSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(16262..17074)
                     /gene="yidA"
                     /locus_tag="KW011_002104"
     CDS             complement(16262..17074)
                     /gene="yidA"
                     /locus_tag="KW011_002104"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBH7660431.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(17189..17587)
                     /locus_tag="KW011_002105"
     CDS             complement(17189..17587)
                     /locus_tag="KW011_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HBH7660432.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17827..20241)
                     /gene="gyrB"
                     /locus_tag="KW011_002106"
     CDS             complement(17827..20241)
                     /gene="gyrB"
                     /locus_tag="KW011_002106"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBH7660433.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(20270..21343)
                     /gene="recF"
                     /locus_tag="KW011_002107"
     CDS             complement(20270..21343)
                     /gene="recF"
                     /locus_tag="KW011_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBH7660434.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(21343..22443)
                     /gene="dnaN"
                     /locus_tag="KW011_002108"
     CDS             complement(21343..22443)
                     /gene="dnaN"
                     /locus_tag="KW011_002108"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBH7660435.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(22448..23851)
                     /gene="dnaA"
                     /locus_tag="KW011_002109"
     CDS             complement(22448..23851)
                     /gene="dnaA"
                     /locus_tag="KW011_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBH7660436.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            24148..24228
                     /locus_tag="KW011_002110"
     CDS             24148..24228
                     /locus_tag="KW011_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660437.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            24458..24598
                     /gene="rpmH"
                     /locus_tag="KW011_002111"
     CDS             24458..24598
                     /gene="rpmH"
                     /locus_tag="KW011_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBH7660438.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            24615..24974
                     /gene="rnpA"
                     /locus_tag="KW011_002112"
     CDS             24615..24974
                     /gene="rnpA"
                     /locus_tag="KW011_002112"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBH7660439.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24938..25195
                     /gene="yidD"
                     /locus_tag="KW011_002113"
     CDS             24938..25195
                     /gene="yidD"
                     /locus_tag="KW011_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBH7660440.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            25198..26844
                     /gene="yidC"
                     /locus_tag="KW011_002114"
     CDS             25198..26844
                     /gene="yidC"
                     /locus_tag="KW011_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBH7660441.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26950..28314
                     /gene="mnmE"
                     /locus_tag="KW011_002115"
     CDS             26950..28314
                     /gene="mnmE"
                     /locus_tag="KW011_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBH7660442.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6864 a         7665 c         7246 g         6682 t
ORIGIN      
        1 tttaagccat tgcgtttcag tggcattttt ccactcaacc agccaggaag ccattttccc
       61 gtaagggatg tatttattac cggcctcatg attctcattc tgacgtgcca gagcacgatt
      121 gaaaacgaaa cgacatgcgc ctgcgaagcg cctcatttca cgctcctgtt gaccaccggg
      181 tcttaactgg aatttaaatg cttgtagtcg tttcacaggc caaatgatac tttggtctgt
      241 gaaaaaagaa accgatatcc ggcgtggaag gcattgtgtt ttcctgatgc atgttcacct
      301 ggtctttgtc accaaatacc gacgtcagat ttttgactat gacgcgacag aaaaactacg
      361 cacttacttt tcaaatgtat gtgctgattt tgaagctgaa ctggtttaaa tggatggcga
      421 accagatcac gtccatttgt taattaacta tcctcccaaa ctggcgatat ccagtctggt
      481 aaacagcctc aaaggcgtat cgggtaggtt actgcgacga gatcgaccag atattgcagt
      541 caggtattac tacaaaggcg ttttgtggag tcctggctat tttgccaata gctgcggagg
      601 tgcgccaata tccgtcatcc gccaatacat tgaacaacag caaacacctg gttaggtgga
      661 aaaccgcgcc ttatatcccc gccctgaggg acggggtttt acggcgcacc ggataaattt
      721 ggttatgcgc cgtcagccgt gctaaagaat acggaccagc acccaacgga tgccagtcca
      781 cacaattaac gtaactctgg ccagtacgct ttgttggcct ccagcaactc atccagaata
      841 gctttggcga ccgaggcgct cggtaccgtt ttcgataacg taatcgcctg ccacaggtgc
      901 tgatatgaac gctgttccca ggcgtccacg accagttttt ccaccgccac ttgctgactc
      961 attaaccctt tctgaaactg cgggatatcg ccgaccacca gtggttctgg tccgttgtgc
     1021 ccgaccagac acgggatctc gaccatcgct tcatcatcaa agttatggat tgccccgttg
     1081 ttaggcacaa tcagcaacat ccgctcctga gtgttgaagg caattgccgc cgccagatcg
     1141 acgatgtatg acgcatgttc gtcaatctcc agcttggccg ccgctgaatt tcctgccgct
     1201 gtaatggcgc ggcaagcatc gaaaacctgt ttctcgcgat gttccattca cctcattcgc
     1261 gcgggtatgt tcagggttgg aatgctgaac aacataatcc gggaagagat aatatttcag
     1321 ataggtgttc ggcaatgtat ccgggtccag tgcctggaca tcccgcgctt ttgcataggt
     1381 gtcgttccag ctcgcttcag tatgtgtatc ttgctgcggt ggaacataac cgtgcttcga
     1441 gacatattgc cgcagctggg gcatcaggtc gttgccctgc aaatcttcaa ttgatgtcca
     1501 ccagccaaag tggttcaggc cgtagtagcg cacgcgcatc tgtttgcgat cttgcagccc
     1561 aacaatttgc gccatccggc tttcaatacc gattggcatg tcacagatgt tgaggatttt
     1621 cgcattcggg cgcagacgac gcgtggcttc tgcgacaatg gctgccgggt tggagtagtt
     1681 gagcatccag gcatttggtg aatatttttc catataatcc accagttcca ggacgccgcc
     1741 aatggaacgc atgccgtacg ctattccgcc gggtccgcaa gtttcctgac caacaacacc
     1801 gtggcgcagt gggatttttt catccagttc gcgcatcggg tacttgccga cgcggatgtg
     1861 cgccatgaca aaatcaacgt cgctgaacgc cacttcagga tcggtggtgt aactaaacgc
     1921 aatgtccggc gctttctctt taaggatgac tttacaggct tcggcaatca cttcctgccg
     1981 cgcgccatcg ttatcataaa atttcagtgc acgaagcggg aaacgctcct gattcgccag
     2041 gagcatcaac acgatgcctg gcgtaaaggt gcttccaccg cctgcgacaa ccactgagaa
     2101 tttggtcatt atactgcctc cgtaatggca acattttctg ctgattgatg agaattaatt
     2161 aagctatcga gctgttcacg cagctgggat acatgcagac caatgatcac ctgaatggca
     2221 tcgccactac ggaagacgcc gtgcgctccc agctttttaa agacttcgtc atccagcgtt
     2281 tgtgacatgt catgcagtgc aatgcgtaga cgcgtcgcac aattgttaat gctggagata
     2341 ttgccgaccc cgcccagggc ttgcaggata ccggcagctt gatccagctc tttttttggc
     2401 tctgccgcgg tggtttggcc tcgcgaggct ttgtattcgg cttttgagta gagtttcact
     2461 tccgcatctt cacgtcccgg cgtgcacatg ttgaactgca gaatcagggt gcggaaaacc
     2521 acgaagtaca gcagggtaaa gcacaaccca atggcgattt gggtcagcat catatccgcg
     2581 tggttgctga acatcgggat ccagttttgc ggtaaaacct ggtcaatcag acctccgccc
     2641 atgttgccca ccacaccaaa gagatacatc acggtcgaca ttgaggccgc aagcacagcg
     2701 tgtaccgcaa acagcaacgg tgaaatgaac aggaaggtaa attccagcgg ttcggtaatt
     2761 cccaccagca tggcggttaa ggttgcggga atcagcaagc ccgccacttt tacccgattt
     2821 tccggtgcgg cagtgaagta catcgctaaa gaaatgccca cggcaccaaa gatttttgag
     2881 ttaccgtgca gggcaaaacc gccttccggg aacaacgatt tcagcggctc ggcgctcaaa
     2941 ctgaactctt gcagatgctg cgcccagtac atctgaatgc cgccttcaac agctgccgga
     3001 cccaagataa acggtccgta gatgaagtgg tgtaaaccgg ttgggatcag aatacgttcg
     3061 aggaaggtgt aaacccagac cccaagcgca cccgccgaac gcaggaacgc ttgcagagat
     3121 tcaatccccg tttgtacttt tggccagccg agcaatgtca gccaggcaca ggggatcatc
     3181 accaggaagg cgataatcac cacataagac gtcccctgga aaatgccgag aaaaaccggc
     3241 agttttttat cgaacagacg gttatgcagc gccgtcacaa tgccggaaat gataattgcg
     3301 ccgataatgc tggtatcgag ggttttaatc ccggccatca ttgtcagacc gctacctgcc
     3361 accgcgtcct gagtgaaatc gacgccgaag tagcttcccc aggtcattcc catcgcgttg
     3421 atgaaatagt tccaggtcag gaaactcacc atcaccgcca gacaagcacg tccctgcgct
     3481 tgcttagcaa ggccaatggg taaaccgaca gcaaaaatca gcggcatatt acggaatacc
     3541 gtccaaccgc cctcttcaat aatgtgtacg atttgcgcga ataaactgtt cggatcggtc
     3601 agtgattccc cgacaaacat cgggttttgc agcaagatgg caagacccac cacaatcccg
     3661 gcgaagggaa acagcagcac tggcgtaaac atcgcgccgc caaagcgttg aatttgactg
     3721 agcatttcca catcctcttt tctcaattct gtaagtagga agcctgattg gttatgcggc
     3781 aggcctggag caaggatagg aagatgcgat cggcagaaca tgacgaccca atgggattcg
     3841 tgatcgcttt catgcttttc gttgggcggt agagatctac gttttcgcga aaaagtagac
     3901 atctgtgagc acaggtacac agcatcgaac acatctttaa aaaaaagatg ttttttcaat
     3961 cgattaagca gaacttgtgt gcgcattacc cgggcttgca ggcaaaaaag agatctagag
     4021 atgatctaca aaagcattgc ggagcggtta agaattcgac ttaactccgc agatttcacg
     4081 ctaaacagcc ttcttccggg tgaaaaaaag ctggcggaag agtttgcggt atcgcggatg
     4141 accatccgta aagccattga cctgctggta gcgtgggggc tggtggtccg ccgccacggt
     4201 agtggcactt acctggtgcg caaagatgta ctgcatcaaa ccgccagtct gaccgggctg
     4261 gtggaggtgt taaaacggca gggaaaaact gtcaccagcc aggtgctgat ttttgaaatc
     4321 atgcctgcgc ctccggccat tgccagccag ttacggattc aaatcaacga gcagatctac
     4381 ttctcccgtc gcgttcgttt tgtggaaggg aaaccgctga tgctggaaga cagctatatg
     4441 ccggtaaaac tgttccgtaa tctttcgctg caacatctgg aagggtcgaa gtttgaatat
     4501 attgaacaag agtgcgggat tttgattggc ggtaattatg aaagcctgac gccggtgctc
     4561 gccgatagac tgctggcgcg gcaaatgaag gtagcggaac acacgccact gctgcggatc
     4621 acctcgttgt catatagcga gagcggggag tttttgaatt attcagtgat gttcagaaat
     4681 gccagcgaat accaggtgga gtaccattta cggcgactcc acccggaaaa gagttaaccg
     4741 atactccaga agagcaccgc cagtaattgg ggggtgataa tgcgcaggaa catcactaac
     4801 ggatagacag tggcgtaaga gagcgccgcc gcaccgctag ttggatgaag attattagca
     4861 aacgccagcg ccggaggatc ggtcatggaa cctgccagca tcccgcacat ggtcaggtaa
     4921 ttcattttgg ctaacatccg cgccagaatg ccaacagtaa tcagcggaac ggcggtgatc
     4981 agggcaccat aaccaatcca gcttagccct tcgccattga ccagggtatt cacaaaatcc
     5041 ccaccagatt tcagaccaac gaccgagagg aacagcacga tccccagctc ccgcagcgcg
     5101 aggttggcac ttggcggcat aaaccagtac agcttgccga tactgccgat acgcccgagg
     5161 atcaacgcca taatcagcag accgcccgcc agccccagtt tcaacgcggc cgggaatccc
     5221 ggcacaaaga cgggaataga acctaacaat acgccaagcc cgatgccaat aaacactggc
     5281 agcatctgaa cctgttgcag tttttgctgc gcattcccca gcacattggc aacggcatca
     5341 attgcggacg gacgccccac caggttgagg atatcgccga actgcaggct gatatcgcca
     5401 ctggcgacca gttcgacccc ggcacggttc aggcgcgaga taacaacgtc atagcgttct
     5461 ttaaagtgca ggtcgcgaat acgttttccg agcacgtttt cattggtgac caccacacgc
     5521 tcgacgcgca aatcagtgtc tttcgtggac agcgaagtat cgacctcctg accaatcacc
     5581 agttgcgcat tatgtaaatc cgctggctga cccaccagat gcagcaaatc gcccagttgg
     5641 ataatggtat ctggcgaagg aacttttagg gtttcttcgc gtttcagacg cgagcagata
     5701 attttgtcgc cgttgagaat cggtacatct ttaatggcta aatcatgcag gttagggttc
     5761 tcaacgcgaa tattgatagt cttgatcagc gcgccgccat tggtgcgtga agactcgtgc
     5821 tgctgagctt ctgtctcgac attgacgcgg aaaataaccc gcaacatcca catggtgaac
     5881 aaaatcccgc aaatgccgaa tggatacgcc atcgcgtaac tcatccccat ctgatcgacc
     5941 atttccattg gtgtacccag gtcgcgcaaa atctgctgcc ctgcccccag cgctggcgta
     6001 ttggtaaccg caccggagaa aatccccagc actaccggca gtggaatatc aaacagttta
     6061 tgcaggatgg cggtaaccag accaccgatg atgacgatca gaacagcaaa caggttgagg
     6121 cgtaatccgg agacgcgcaa tgaggcaaag aagcccggcc ctacctgaat cccgatggta
     6181 taaacgaaca ggatcaggcc aaattcctga ataacatgca gcatatcgct acttaatgtc
     6241 atccccgcct gagaaacaaa atggccgacg atgatcccac caaacagcac gccgccaata
     6301 cctaatccta tgccgcgaaa tttgacgttg ccgataaaca aaccgacgac tgccaccaaa
     6361 gccagaatac tgaccgttaa tgctatatca ctcatcgtcc atttctcttg agataacatt
     6421 ttaattgcta cggattatgg ctgaatcggt gaagcagatt tgccttatag cgcacattat
     6481 tacgggaaag aacgtgccga aatatcttaa acagtcgaaa tttaacgcat tgttgaagaa
     6541 gaaagacaaa aaaagcccca ccagaatggc ggggcaaaga gaatagctag ttagctattt
     6601 aacgcgggac gttcgctgat agcgatacgc tgcgctgcga tgggttcagg ctcattacga
     6661 attaaatcaa tatgcagtaa accgtttacg aaagttgcgc cagagacttc catattttca
     6721 gccagcgtaa agctcaggct aaatggctga ttcataagcc cttgatgcag ccattttttc
     6781 tcttcttttg gctgctccgg cgtgcctttt acgctcaggc gcgtaccttc cagttgaatc
     6841 tctaaatctt cctgacggaa acctgccagc gcaagggtaa tgcggtagtg gttatcgtcg
     6901 cttttctcaa tgttgtacgg cgggaagctc tgactttcac cggcgttttg cagtgcgttg
     6961 gccagtttgt caaaaccgat ccattgacgc atcagtgggg ataaatcgaa gttacgcata
     7021 gtcatttctc cttctaagaa gcgagtaagt acctgcaaat ccgaagattc gcatatgctc
     7081 cctgacggcg agcatggaga tgtcaggccg cgccaggcgg ccttaaggaa ttagttgatt
     7141 tcgatacggc gcggtttttt cgcttccgga atcacgcgtt cgagatcgat atacagcaaa
     7201 ccatttacca ggttagcgcc acgaacatga atgttctcag ctaactggaa tttgcgttca
     7261 aagttgcgtt cagcgatgcc ctggtacaga taggtgcgct ctttttgttc gtcggcgtga
     7321 gcacctttca ccaccagcag attatcctgg gcggtaattt ccagttcgct ctcagcaaaa
     7381 ccagccacag cgatagcaat gcggtaatgg ttttcgtcta ccagttcaac gttatacgga
     7441 gggtagccgc cattactctg gctctggttg ttttctaagt ggttaaacaa acggtcaaat
     7501 ccaatagcag aacggtaaag cggggataaa tcaaagttac gcataatcaa tagctcctga
     7561 aatcagcgag aatgtaagac cttccacaat ggacaggtca ggtagccaga acacccatca
     7621 ggcgcgtcct catcgactac gatgtaaaaa tgggtctgga aatgaacttt tcaagcctta
     7681 tcagcggact tttttttgca gtttatggtc tattgcatag actgaggggg cagcacgaaa
     7741 cgttaaagtg cgatagccgc cacagagcga cgtaatgatg atgttatttt cagcaaggga
     7801 tcgctataac tcatcaggcc aacacccacg atcaacagat gaatttatta tgataagaaa
     7861 tgtgttgtta gcgttcatga tatgcagcgg aatggcatta ctcggaggat gctccagcgt
     7921 gatgtcccat accggaggga aagaaggaac gtatcccggc acccgcgcca gcgccaccat
     7981 gattggcgat gatgaaacca actggggcac taagtcgctg gcgattctcg atatgccgtt
     8041 taccgctgtg atggacacgc ttttattacc gtgggatgtg ttccgcaaag acagctcggt
     8101 gcgctcacgc gttgaaaaaa gcgaagccaa cgcgcaagcc accaatgcgg ttattccacc
     8161 ggccagaatg cctgataact gatttagcga tcagtttcgg tgatccacag ctggccacct
     8221 tccatccacg ccagccattg accatccggc gagaagacca cggcgtccgc agaaggtgga
     8281 ttggcgtggt tttcggttaa atactcaacc tcgccgcttt gcgcatgggc acaagcaatt
     8341 cgattatcca gcacaaagcc caaccattct cctgacggat gccagttaaa tgcagactga
     8401 atatccgttt tgttatgggt taactggcgc ggctcgccgc cctgtggcga gataagccac
     8461 agttgcacaa tgccgttatc atcacgcatt aaaaacgcga tttgcgtacc ctgcggatta
     8521 cagcgcaccc agtggcgcgg gacgttgact aaccccggat aagcccgatg gtgggtaaag
     8581 gttaaacgtc gctgcacgac gccacgcggt ggcgcgggca gggtggtttc cgttccactt
     8641 aacggcgcat cacctgccgc tttccagcca gcttcatctt gcggcaactc aacgataaac
     8701 agctccggca ctttctcacc ctttggcgaa agtgtgtcgc caataaacgc cagcgcgtga
     8761 tttcctaccc atccttcttc ataagcacga ttgatttcat cactgccagg ctgcggcgtg
     8821 ggcgtggttt tactcaccag cacacaccag tggctgccgc tgtattcacg cggatgctgc
     8881 ttttgtacgt tgaccggacc aaacggcgca gcaacaccga cgtttcgcaa atccagcgcc
     8941 ggatcgagtt catgcattac atggtcgtta taggtaaagc tcaccctttc accgttcggg
     9001 ctaaagacat gcacatggct gccgccgcgc agcgctcctg gcgtatacgg cgcggtaata
     9061 tccattgcat cgagattgct caccttgccg ccttcagcaa tgactccgcg ccgatgatgg
     9121 aagtcgtaat gccatgtttc atcaggattt tccgggccgt gaataaagac atatttctct
     9181 gacttcggat gaacggtcac cacgccgacg tgtgcgccct gtgacgcaca gtagataacc
     9241 tcgacctcac cggtatggat attcacacgc tcaatggtct cgccggtaaa cgacgcccca
     9301 gaaggacgca cgtcaaatac cagccactgg ctgtcgggcg tccaggtatt ggtattggtg
     9361 agcaggtgat tacggggagc aaaggttatc tgtttcatgg cgcggtcctg atacaaaacg
     9421 ggtccagcca tcatacttca caaggacttc actttcagac aatagtcttt tgccatcgtg
     9481 atgaatgtga gtgaaaagat ggaacatttc gacgtggcga ttattggcct cggcccggca
     9541 gggtcggcgt tggcacgaaa gttagccggc aaaatgcagg tgatcgcgct ggataaaaag
     9601 caccagcatg gtactgaagg tttcagcaaa ccttgtggcg gtctgctggc accggacgcg
     9661 cagcgatctt ttattcgcga tgggctgacg cttcctgtcg atgtgatcgc taatccgcag
     9721 attttcagcg tcaaaaccgt cgacgtcgcc gcatcgctca cgcgtaacta ccagcgaagc
     9781 tatatcaata ttaatcgcca tgctttcgac ttgtggatga aatcgctgat ccccgccagc
     9841 gttgaggttt atcacgacag cctgtgtcga aaaatctggc gtgaggatga taaatggcat
     9901 gtcatttttc gtgcagacgg ctgggagcag catattaccg cccgctatct ggtcggtgcc
     9961 gatggtgcca actcgatggt gcggcgacat ctctacccgg atcatcaaat ccgtaaatat
    10021 gtcgcaatcc agcagtggtt cgcagagaaa catccggtgc cgttctactc ctgcatcttt
    10081 gataatgcga taactgactg ttattcatgg agtatcagca aagacggtta ttttatcttt
    10141 ggcggtgcct atccaatgaa agacggtcag acgcgtttca cgactctgaa agagaaaatg
    10201 agcgcctttc agttccagtt tggcaaagcg gtgaaaagcg aaaaatgcac ggtgctgttt
    10261 ccctcacgct ggcaggattt tgtctgcggt aaggacaacg cctttctgat tggtgaagcg
    10321 gcgggattta tcagcgccag ctcgctggag gggattagct atgcgctgga tagcgcagac
    10381 attctgcgtt cggtgttact gaagcagcca gagaagctca ataccgctta ctggcgcgcc
    10441 acccgcaaac tgcgtttaaa actcttcggc aagatagtaa aaagccgatg cctgaccgca
    10501 ccggctttaa gaaagtggat tatgcgcagt ggtgtggcac atattccaca gttgaaggat
    10561 tagccaacgc gcttcacatc gcccaccagc aggatataag agagcgcgcc aatcaacgcg
    10621 acggcggaga tataaaccag tgcaggtgcg aaaccgtaac cctgcgccag gtaccccacc
    10681 accagcggaa cggtgatgcc gcccagacca ccggcgaagt taaacacgcc gccggttaaa
    10741 ccaatcaggc gcatcggtgc cagagaagaa accagcgacc aggtaatcga agcaaaaccg
    10801 ttaccgaaga atgccagcgc catcaggcac ataatcatca tcggatcgtt agtgtagtta
    10861 gcgcccataa tgcaggtgga gatcagcaag ccgcagataa tcggcgtttt acgcgcaaag
    10921 cccagtgaaa agcccttacg taccagcaga tccgctaccc agccagagag caggacgccg
    10981 acaaacgccg cgaggaatgg caccgtggtc ataaagcctg ctttcagcgc cgtgattcct
    11041 ttttcctggg ttaaatagtt cgggaaccag gttaagaaaa accacagtgt agaagccacc
    11101 gcaaattgcc caagatagac gccgatcagt ttacgatgga acaccagttt ccagtctttg
    11161 gctgttaacg gctgacgtgc ctctttcttc actggcgcat caccatccac cagaccgccg
    11221 ccatcacgaa tgtaatccag ttcagctttg ctgatgcctt tggtcaggcg cggcggctga
    11281 taaaccttaa accaaatcag cgaccagata atgccgatac caccagtcac aatgaacacc
    11341 cagtgccagc tcaacatctc ctgaatccag atcagcagcg gagtcaaaaa cgccagaccg
    11401 acaaactgac cagacgtata aaaaccaacg gcggaagcgc gttcatgttc cgggaaccag
    11461 ctggtcacca tccggttatt ggtcgggaaa gcgggcgctt cgaaaatacc ggttatcgca
    11521 cgcagaccaa ttaatgacat taatcccgtg gcaaagccct ggaataaagt cgccactgac
    11581 cagccaaata tcgcaataaa ataagtcacg cgagaaccta cgcgatctaa aaaccaaccg
    11641 ccggggatct ggcatagcgt ataaagccag gcgaaggccg aaaatacata gcccatttcc
    11701 gctttcgtaa tgccgaactc ttcctgaata tgggcggaag ccacggccag gttagcgcgg
    11761 tcaacataac aaatgaccac cgtaataaag atcatcacca gcgtcagata acgccgacgc
    11821 cccggctttg ctgcattaac gggaatatcc atagcgagct ttctccagat tttgggcata
    11881 gcgaagccgc tcaccatgcc ctgtaattta cagagggtta tatttttgta ttgctgtttt
    11941 agtgcccgat gaggggctta cgtggcagga attaccactc tgctacgctg ttatcttcat
    12001 gacgccagag tggattacgc cagtccgggg catttttact gaactcaata actttagctt
    12061 cgtcgatttc cacgcctaag cccggtttcg ttagcggttt aaagaagccg ccaaccatgc
    12121 tgaagtcttc tttgtttttc acaaagtcga gtaactccgc gcctttgttg taatgaattc
    12181 ccatactttg ttcctgaagt accgcgttat aggaaacaaa gtcgatatgc agacaagccg
    12241 ccagtgcaat cggtccgagc ggacagtgcg gcgcaagggt cacgtcatag gcttctgcca
    12301 ttccggcaat tttgtagcat tcggtaatac cgcctgcatg ggagagatcc ggttgtagaa
    12361 tcgaaatacc gcctgcctcc agcacgcgtt taaaatcgaa acgtgagaac atgcgctcac
    12421 ccgccgccag tggaatatgc gtttgtgccg ccaatttcgg gtagtattcg gcttgttccg
    12481 ccagcaccgg ctcttcaata aacagcgggc gatacggctc cagttcttta atcagcactt
    12541 tcgccattgg cgcgctgacg cgaccgtgga aatcaagacc aaactcaatc tgattgccaa
    12601 aagcttcacg aatttgtgcc acggtgttga ctgccgcatc taccgcgcgg gagttatcaa
    12661 ttagccccag ttcttcacaa ccgttcagtt tgaaggtatc gaagccgatt tcgcgcaggg
    12721 ttttaatacc gtcgatcaca tccgccggac gatcgccgcc gacccaactg taggctttaa
    12781 ttttgtcgcg aaccaggccg cccatcagtt gccagaccga cgcattcagc actttacctt
    12841 tgatatccca taacgcctgg tcgatcccgg caatggcgct catcaggatt ggaccgccac
    12901 gataaaatcc ggcgcgatac atcacttgcc ataagtcatt gatgcgctat ggatcctgac
    12961 caatcaaata gtcacccagc tcgtgaactg ccgcttccac cgtgcgggcg cggccttcaa
    13021 tcactggctc gccccagccg acgacgcctt catcggtttc aattttcagg aacatccagc
    13081 ggggaggtaa acgatacgtg gtaattttgg tgattttcat tgcactgcct ctcgatacgc
    13141 cttaacaaat gctgctgcct gctgcgcggt gcgttctacg gactgcccgg cgcgatagag
    13201 atcgctgcct aagcccgccc cagcacaacc tgcgtctatc cactgcgcca ggttttctgg
    13261 cgtcacgccg ccaacggcaa agactgcgat gtccgatggc aataccgctt ttaacgcttt
    13321 gatgtattgc ggaccaaaag ccgatgacgg aaatattttc agcgcctgcg cgcccgcttc
    13381 gagcgcggta aaggcttcgg tcgccgtcgc gcagccgggg cagacggtca tgccgtagcc
    13441 caccgcacgg cggatcactt cactatggat attgggcgta acgatgagct gacagcccat
    13501 cctggcgagc gcatcgacct gttcaggttt cagtaccgta cctgcgccaa tcaacgcctt
    13561 gtcgccatac gcatcaacga tggcgggaat gctttgctcc cattgtgggg aattcagcgg
    13621 gatttcaacc gcgtcgaacc cggcgtcaat caccgcgcca acatgcgcca gcgcctcgtc
    13681 gggcttaatg ccgcgcaaaa tggcgatcag cggaagttta gtttgccact gcatgagcga
    13741 tgctccttat accagcctga aatgccgtgt cgcccgccac cgccgtcacg tcgcaaccca
    13801 tcgcctgaaa ggcttgctgg tagcgcgcgg tcaacgatgt tccggcgaca agggtgatgg
    13861 cgtgttgatg ggccacatag tcgcgcatac tggcgacctc tgcgccaatc aacaaaccag
    13921 agagaaattc actgacctgt tcacggggaa gtgttcccag cacatgcgag gcacgaactt
    13981 caaaaagctg cggcaatatg gcgggcgtat taagaccacg ctcaaggcca gccgtgaagg
    14041 catcggcaga gttttcctgc ggcggcaaac ctgcaccaat caatgagtga tttaacagta
    14101 aatgatgtaa ttcaccggtc atcacggtgc gaaaatcgtt gatttgctgg ctatcggcct
    14161 gcacccattt gcaatgcgta ccgggcatga cataaagaga ggaaggagcc agagctcgcg
    14221 cgccgatcaa ttgagtttct tcgccgcgca tcacattgtg gttatcatca tgagagacgc
    14281 ataatccggg aataatccag atattgtcgc caacagacgt taattgttcg ccaatagacg
    14341 aaaaacgggc aggaacagat aaatacggag caactttcca gccgacattg ctgccgacca
    14401 ttcctgccat taccactggc gtattctctt cacgccagtc ggtcgtgact tctgctaaca
    14461 ccgcagccgg agattttccg ttcaggcgcg tgacgcctgc ttctgattgc ctgctctcca
    14521 ggcagtggtc gccctgataa agccaggcgc gcagattggt cgatccccag tcaattgcga
    14581 tgtagcgagc tgtcatgtga tttcctttaa ccttcgtgtc gagctggcga tcatggtaag
    14641 cgccgcctgc tctgccgcat cgccgtcctg atgccgtatc gcatcgaaca gcgccttatg
    14701 ttcctggagc gtttgcggca tgttggcctc atcgcccatc caggttcgtt caaaaaccgc
    14761 ccgctgcagc gaactgatcg caatgctaag ttgctgtaac accgggttat gcaccgactg
    14821 cagcaccgcc tcgtggtagc gaatatccgc ttcgttaaac gcttcgcggt cctgattgtt
    14881 ggcaatcatc tcgttcagcg ccgattcaat ctgcgccaga tcgctggagg tcgcgcgctc
    14941 tgctgcccaa cgggcaatcg ccggttccac cagatttcgc acttcgctca tggcactgat
    15001 aagccgtggg tcgtagtcgt tttccagcac ccattgcagt acgtcagtgt cgaggtaatt
    15061 ccactggtta cgcggtgcca caaacgcgcc gcgataacgt ttcatttcaa tcagccgttt
    15121 cgccatcaac gaacggaaca cctcacggat gatgttgcgc gaggttgcaa actcctcaca
    15181 gagttccgcc tcagccggaa gcggcgagcc tggtacgtat ttgccgtgaa cgatctgttt
    15241 acccagcgta atgacgatgc gatcggtttt attgagagtc atggagagtc cttgtgctct
    15301 gtatgttctt ctctacttta ccccgatcga tgcataacac ggcaactttg tagtaccagc
    15361 gtgatgacgt tcgcgtttgc tgtgcgtgta atgtagtaca aacttatatt gttgtactac
    15421 aatttagatc acaaaaagaa caatgcgtaa aaaaatgaca tgcgtcgggc agaaatctga
    15481 aaagggatat caggcgctaa acaggaggga aagaagagta tgctttcaac ggcttagcta
    15541 ctcgtttaaa ggattaatca tgaagttgaa tcttaaggga ttttttaaga ctgccggttt
    15601 attcccactg gcgctgatgc tttcgggctg tatctcgtat gccctggttt cccataccgc
    15661 aaagggtagt tcaggaaagt atcaatcgca gtcagacacc atcacggggc tatcgcaggc
    15721 aaaggatagt aatggaacaa aaggctatgt ttttgtaggg gaatcgctgg attaccttat
    15781 cacagatggt gccgatgaca tcgttaagat gctcaaggat ccagctctta accggcacaa
    15841 tattcaggtt gctgatgacg caagatttgt tttaaatgcg gggaaaaaga aatttaccgg
    15901 cacaatatca ctctattaca actggagcac agaagaagaa aaagcacgag tgacacaata
    15961 tcgttttgct tgtggtgttc agcactgtac ccggacagta gaaaacctgg aaggtactat
    16021 ccatgagaaa aataaaaaca tggattactc aaacgtaatg gctttctacc atccatttaa
    16081 agtgcgattt tatgaatacc attcacccag aggcattccg gatggtgttt ccgcagcatt
    16141 actgccagtg actgttacgc tggacatcat tactgcaccg ctgcaatttc tggttgtata
    16201 tgcagtaaac caataatcag taagcgggca aacgcgttta tgctgtttgc ccgcccacag
    16261 attaattcag cacatacttc tcaatagcaa acgccacgcc atcttcaagg ttagatttgg
    16321 tgacaaagtt cgccacttct ttcaccgaag gaatagcgtt atccatcgcc acaccgacgc
    16381 ctgcatattc aatcattgcg atgtcgtttt cctgatcgcc aatcgccatg atttcttccg
    16441 gtttaatacc taacacgtcg gccagtgatt tcacacccgt acctttgtta acgcgtttat
    16501 cgaggatttc gaggaagtac ggcgcacttt tcagcacggt atatttctct ttcacttcct
    16561 gcggaatacg cgcgatagcc tggtcgagga tggcgggttc atcaatcatc atcactttca
    16621 ggaactgggt attggggtcc attttctccg cttcgcagaa caccagcgga atagtggcaa
    16681 cgaaagattc atgcactgtg tagtagctga tatcgcggtt ggcggtgtac agcgtggtgc
    16741 ggtccagggc gtggaaatga gaaccgactt cgcgagagag tttttccagg aaacgatagt
    16801 cgtcatagct gagagcagtt tgcgccacgg tgctaccatc agcggccttc tgtactagcg
    16861 cgccgttata agtaatgcag tagtcgcccg gctgttccat atgcagctct ttcaggtagt
    16921 tgtgcacacc tgcatacggg cgacccgtcg ttagcacgac attcacgcca cgggcgcgag
    16981 ctgcggcaat cgcattttta acggcgggtg aaatggtgtg atcgggcagc agaagggtgc
    17041 catccatatc gatagcaatg agtttgatag ccatgagttc cccaggtaga ttggttcctg
    17101 acccatgcta acgcgattcc gctcaaaaat cagtacaaca cccgagggaa aagggggatg
    17161 caacgcgggt tgcatgctcc ccatttgctt agcggaagag tttccctttc agcagttcca
    17221 tacctgcgga aagcagatcg ttattggctt gtggtgacac ttcaccttgc ggtgagagcg
    17281 catcaataat cttcggcaat tgctctgcca gtaaactgga agctgtactg gtatccacgc
    17341 caagtttttg cccgagatcg gacaccgcat ttgtgccgag cgccgattcc agttgctcgc
    17401 cactaaccga ttgattgccc tgttgattac tcagccaggt tgagagaatg gcccctaagc
    17461 cgccactttg cagtttttcc agcagcacct gaatgccgcc ctgctcctca acccaactta
    17521 aaatagcctg atatttcccc gcatcgcctt tcagaaaggc accgacaact tcatcaaaaa
    17581 gccccatgat aatcacctgt aaagcgttac gtgttgaccc aaaaagtata gatttgcggg
    17641 atgataattg cggattgcag aaataaaaag ggcggagatg atctccgccc ttttcttata
    17701 gcttcttgcc ggatgcggcg tgaacgcctt atccggccta caaaatcatg aaaattcaat
    17761 acattgcaag attttcgtag gcctgataag cgtagcgcat caggcacgct cgcatggtta
    17821 gcgccattaa atatcgatat tcgccgcttt cagggcgttc tcttcaataa acgcacggcg
    17881 cggttcaacg gcgtcgccca tcagcgtggt gaacaactgg tcggcagcaa tcgcatcttt
    17941 aacggtaacg cgcagcatac gacggctttc cgggtccatg gtggtttccc acagctgttc
    18001 cgggttcatc tcgcccagac ctttataacg ctggatggag aggccgcgac gggactcttt
    18061 caccagccag tccagcgcct gctcgaagct ggctaccggc tgacgacgct cgccacgttc
    18121 gataaacgca tcttcttcca gcaagccacg cagtttctca cccagcgtgc agatacgacg
    18181 atattcgcca ccggtgataa actcgtgatc cagcggatag tcagtatcca caccgtgggt
    18241 acgcacgcga acaatcggtt caaacaggtt ttgttcggca ttggtgtgga catcaaactt
    18301 ccactggctg ccgtgctgtt ctttgtcgtt cagttcgctg accagcgcgt tcacccagcg
    18361 ggtaacggtc tgctcatcag agaggtcggc ttccgtcagc gtcggctgat agataagctc
    18421 tttcagcatt gctttcggat aacgacgctc catgcggttg atcattttct gcgtcgcgtt
    18481 gtactcagac accaatttct ctaacgcttc gccagccaat gccggtgcgc tggcgttggt
    18541 gtgcagcgtt gcgccatcca gcgcgataga gatctggtac tgatccatcg cttcgtcgtc
    18601 tttaatgtac tgttcctgct tgcctttctt cactttgtac agcggcggct gagcgatgta
    18661 gacgtgaccg cgttcaacga tttccggcat ctgacgatag aagaaggtca acagcagcgt
    18721 acgaatgtgc gagccgtcga cgtccgcatc ggtcatgatg atgatgctgt gataacgcag
    18781 tttgtccggg ttgtactcgt cacgaccgat accacagcca agcgcggtga taagcgtcgc
    18841 cacttcctga gaagagagca tcttatcgaa gcgcgctttc tcaacgttga ggattttacc
    18901 cttcagcggc agaatcgcct ggttcttgcg gttacgccct tgcttcgcag agccgcccgc
    18961 ggagtcccct tccaccaggt acagttcgga aagcgccgga tcgcgttcct ggcagtctgc
    19021 cagtttgccc ggcaggcccg ctaagtcgag cgcaccttta cggcgggtca tttcacgcgc
    19081 gcgacgcgcc gcttcacggg cacgggcagc atcgataatt ttgccaacca cgattttcgc
    19141 gtcggttggg ttttccagca ggtattctgc cagcagttcg ttcatctgct gttcaaccgc
    19201 tgatttcacc tcagaagaaa ccagtttgtc tttggtctgg gaggagaatt tcgggtccgg
    19261 cactttcacg gaaacgaccg caatcaggcc ttcacgcgca tcgtcaccgg tggcgctaac
    19321 tttggctttt ttgctgtagc cttctttgtc catgtaggcg ttcagggtac gggtcatcgc
    19381 cgcacggaag cctgccaggt gagtaccgcc gtcacgctgc ggaatgttgt tggtaaagca
    19441 gtagatgttt tcctggaagc catcgttcca ctgcaacgcc acttcgacgc caataccgtc
    19501 tttttcagtg gagaagtaga agatattcgg gtggatcggc gttttgttct tgttcagata
    19561 ttcaacgaac gccttgatgc cgccttcata gtggaagtgg tcttctttgc cgtcgcgctt
    19621 gtcgcgcaga cgaatggaaa cgccggagtt gaggaacgac aactcacgca gacgtttcgc
    19681 cagaatttca tattcgaact cggtcacatt ggtgaaggtt tcgaggctgg gccagaaacg
    19741 caccatggtg ccggtttttt cagtctcgcc ggtaaccgcc aacggtgcct gcggtacacc
    19801 gtgttcgtag atctgacggt gaattttacc ctcgcgctgg ataaccagct ccagtttttg
    19861 cgacagggcg tttactaccg aaacaccaac gccgtgcaga ccgccggaca ctttatagga
    19921 gttatcgtca aatttaccgc ctgcgtgcag aacggtcatg atcacttccg ccgccgatac
    19981 gccctcttcc gggtgaatac cggtcggaat gccgcgcccg tcatcctgta cagagacaga
    20041 gttatcggcg tgaatggtga cgataatttc tttacagtga cccgcgagcg cttcgtcgat
    20101 agcgttatct accacctcga ataccatgtg gtgcagaccg gtgccgtcat ccgtgtcgcc
    20161 gatatacata cccgggcgct tacgcaccgc atccagccct ttcaggactt tgatactgga
    20221 ggagtcataa gaattcgaca tcaacgtttc tcgctcattt atacttgggt taatccgtta
    20281 ttttaccctt ttccacggta aacatcttcg aattttcgtc cgacatgtct ataacgtgtt
    20341 cagcactgat cgcgctgaca aagacctgtg attgcgtcgc ttttaagcgg ctggcaagca
    20401 gcccgcgacg ctcatcatca agctcagagg caaaatcatc tatcaggtag agacaccgcc
    20461 gcccgctttc acgggtgagg aactctcctt gcgccagacg taaggcgcac atcaacagct
    20521 taagctgccc acgcgataag gtatcttcca ccggcgcacc gtcggcgcga atgcgtaagt
    20581 ccgctttatg cgggccatgc gcggtgtagg ttagctggcg atcgcgttca aaattacgtt
    20641 ccagcacctc agcatattct gtctctttct cccagccgcg ctggaaagag aaagtcagag
    20701 aaaactcagg gagaaattgc ttacaagtat cggccatatc agccgcgata ccggcgctat
    20761 actccgcgcg ccaggtgctg atttgctccg ccagcgggat cagctcttta tcccacgggc
    20821 gtagctgttc gtaacgtgtc acctggcgca gcgccgcatt gcgctgcttg agcaatcgct
    20881 tgagattgct ccaggcggtg aaaaatccgg gttcgttgtg aaagcatccc cagtcgagga
    20941 atgctcttct gtatttgggg ccgccgttga gtaaagtaaa cccttctggc gttatcaact
    21001 gcattggcat caggtgcgcc agttccgcga ccttatgccc gtctgtaccg tcgatgcgga
    21061 ctttgctgtc gccctgtttg tctttggtta agccaatcgc tgtctcgcgc tcttcgccct
    21121 gtaatcgccc gtggagaaca aacgcctcct gctcatggcg aatgacgcga ccaatctgca
    21181 aactgcgaaa cgcccgacca tggccgagcg tatagatggc ttccagcacg ctggttttgc
    21241 cactgccgtt ggcacctacc agaaagttaa agccgggaga taaagcgaga tccgcggttt
    21301 caatgttgcg gaaatcgcgg atcaacaagc gggtgaggga cattacagtc tcattggcat
    21361 gacaacatag gcagccgact gtgatgcggc atcttcaatc tgcacgctgg aaaccgaatc
    21421 ggtcagcatc atgcggacgt tttcacattt cagcgcgttc agaacatcca gcacgtagct
    21481 gacgttgaag ccgatttcca tctccgcacc gctataggta acgtcgagga tctcttccgc
    21541 ttcttcctgt tccgggttgt tggcggtgat tttcagctgg ttttcgctga cataaagacg
    21601 tacgccgcgg aatttctcgt tagagagaat cgccgcgcga gcaaacgcct gcttgagcag
    21661 atcgcagcca gcttccagat gtttgtccgg gttcttcggc agaacgcggc gataatccgg
    21721 gaagcgacca tccaccagtt tggaggtgaa gataaagtcg ccaacgtggg cgcgaatgtt
    21781 gttgctgcca atctgtacgc gcagcggatt gtcgccgccg tcgagcatac gcatcagttc
    21841 aatcacgcct ttacgcggca cgatcaccga atggcttggc aaagattgac caattggcat
    21901 tgaacagact gccagacggt ggccgtcggt cgccacggtg cgcagttctt cgccttcggt
    21961 ttcaaacagc ataccgttta agtaatagcg aacgtcctga tgcgccatag aaaactgggt
    22021 cgcttcaatc agacgcttca tcgtcgcctg cggcagggta aattcgactt cgctctgcca
    22081 gtcatcgagg ttcgggaaat ccgccgctgg cagggtagac agcgaaaaac ggctacgccc
    22141 ggagcgcacc agcatccgtt caccttccag ctgtacggca atttccgcgc cttcaggcag
    22201 accacggcag atatcaaaga atttgcgcgc cggaacggtc gtcgctcctg gctcgtgtgg
    22261 ctgaaccagc gcaacgcgtg ccaccatttc catctcgaga tcagtaccgg tcagcgacaa
    22321 cgtaccgtca gcaacctgta acagcagatt accgagaatc ggtagcgtag gacgaccacc
    22381 taacggcccg ctcacctgtt gtagcggttt taataaatgc tcacgttcta cggtaaattt
    22441 cataggttta cgatgacaat gttctgatta aatttgaaaa atcttctttg atatcgtggc
    22501 tctcttcacg caactgctcg atcttacggc aggcatgaag caccgtcgtg tggtcacgac
    22561 caccaaacgc atcgccaatc tccggcagac tgtggttagt cagctctttc gccagcgcca
    22621 tcgccatctg gcgcggacgc gccaccgagc gggatcgacg cttggaaagg agatccgcga
    22681 ctttgatctt gtagtactcc gccaccgtct tctgaatatt gtcgatggtg accagttttt
    22741 cctgcaatgc cagcaagtcg cgcagtgcct cacgcacgaa gtcgatggtg atcgcccgtc
    22801 cggtaaagtt ggcgttagca ataacgcggt tcagcgcccc ttccagctca cgtacgttag
    22861 atcgtagacg cttggcgata aagaacgcca cttcgcctgg caaacgaatg tcattttcgt
    22921 cggccttttt catcaggatc gccacgcggg tttccagctc tggcggttcg atcgccacag
    22981 tcagtcccca accgaagcgg gatttcaaac gatcctcaac gccgttgatc tctttcggat
    23041 agcgatccga ggtgagaatg atctgttgat taccttccag cagggcgttg aaggtgtgga
    23101 aaaactcttc ctgagatcgt tctttattag caaaaaactg aatatcgtcg atcagcagtg
    23161 catctacgga acggtagtag cgtttaaact cttcgatcgc gttgttttgc agggctttaa
    23221 ccatgtcctg aacaaagcgc tcggagtgca tataaaccac tttggcattc ggcttgcgcg
    23281 ccataatgcc gttacccacc gcatgcagca ggtgagtttt acccagaccc gtgccgccat
    23341 aaaggaacaa tgggttatag gcaccgccag ggttatccgc cacctggcga gccgccgcgc
    23401 gcgccagttg gttagattta ccttcaacga agttatcaaa cgtgtgtttg acgtttacgt
    23461 tagaacgata ggtcggttct gccggggccg ggacgttatc ccaacctgag cgcgtagaag
    23521 gcgcagcacg ttgcggctgc gtttgcgcca cctgtgcagg ggccgcgacg ttgctcgtca
    23581 ctgccgcttg tggcgtttgc gtcaccggtt tggtgccgac ttcaaaacgc agctgtgggg
    23641 catccgctcc gcagaaactg gttagcagtc cattgatatt attaaggtac ttgtcccgta
    23701 cccaatcgag gacaaaacgg tttggcgcgt acagggccag cgtgttatcg ctcagttccg
    23761 cctgcaatgg gcgtatccac atactgaatt ctgtggctgg taactcatcc tgcaatcggg
    23821 caagacactg ctgccaaagc gaaagtgaca cggcggactc cactcgaaca aaagtcgata
    23881 atgactaagg ctgaaacatt catgattgtt gacgtacgtc gaaaagaccc tgcttgaggg
    23941 tgacgcacga accgctgtct gcggttatat gcccgatcaa gatcctgcaa aacgatcggg
    24001 accgcggatc atagcctaaa ctgcgcaaga gatcttctgt ttctcacaga ttttcccgat
    24061 ttatccacag gactttccag acctcgctaa gtgtaaacga tcctgccgcg aggcgggcac
    24121 gatttacgcc gcatattgga aaatttaatg accatagaca aaaattggct taatcgatct
    24181 aataaagatc taggacgatc cttgcgcttt acccatcagc ccgtataatc ctccacccgg
    24241 cgcgccatgc tggttttcac tggtgtgagg tcgtacattt tccctgcgaa aaggtgcgga
    24301 aaagcgcggt aaataaggaa agagaattga ctccggagtg tacaattatt acaatccggc
    24361 ctctttaatc acccatggct tcggtgtcca tcgtttcatt tttcggcgga tatccaataa
    24421 agccattgaa tttattcaag tttaggtaga aatcgccatg aaacgcactt ttcaaccgtc
    24481 tgtactgaag cgcaaccgtt ctcacggctt ccgtgctcgt atggctacta aaaatggtcg
    24541 tcaggttctg gcacgtcgtc gtgctaaagg ccgcgctcgt ctgaccgttt ctaagtaata
    24601 aagctaaccc ctgagtggtt aagctcgcat ttcccaggga gttacgcttg ttaactccca
    24661 gtcaattcac attcgtcttc cagcagccac aacgggctgg cacgccgcaa attaccattc
    24721 tcggccgcct gaattcgctg gggcatcccc gtatcggtct tacagtcgcc aagaaaaacg
    24781 ttcgacgcgc ccatgaacgc aatcggatta aacgtctgac gcgtgaaagc ttccgtctgc
    24841 gccaacatga actcccggct atggatttcg tggtggtggc gaaaaaaggg gttgccgacc
    24901 tcgataaccg tgctctctcg gaagcgttgg aaaaattatg gcgccgccac tgtcgcctgg
    24961 ctcgcgggtc ctgatagccc tcattcgggt ctatcaacgc ctgattagtc cgctactcgg
    25021 gccgcattgt cgtttcactc caacctgttc aagctacgga attgaggcat tgcgcaggtt
    25081 tggagtgata aaaggcagtt ggttgacggt gaaacgcgta ttaaaatgcc accctttaca
    25141 ccctggtggt gacgatcccg tcccgcccgg accatttgat accagagaac actaacgatg
    25201 gattcgcaac gcaatctttt agtcatcgct ttgctgttcg tgtctttcat gatctggcaa
    25261 gcctgggagc aggataaaaa cccgcaacct caggcccaac agaccacgca gacaacgacc
    25321 accgcagcgg gtagcgccgc cgaccagggc gtaccggcca gtggccaggg gaaactgatc
    25381 tcggttaaga ccgacgtgct tgatctgacc atcaacaccc gtggtggtga tgttgagcaa
    25441 gctctgctgc ctgcttaccc gaaagagctg aactctaccc agccgttcca gctgctggaa
    25501 acttcaccgc agtttattta tcaggcacag agcggtctga ccggtcgtga tggcccggat
    25561 aacccggcta acggcccgcg tccgctgtat aacgttgaaa aagacgctta tgtgctggct
    25621 gaaggtcaaa acgaactgca ggtgccgatg acgtataccg acgcggcagg caacacgttt
    25681 accaaaacgt ttgtcctgaa acgtggtgat tacgctgtca acgtcaacta caacgtgcag
    25741 aacgctggcg agaaaccgct ggaaatctcc acctttggtc agttgaagca atccatcact
    25801 ctgccaccgc atctcgatac cggaagcagc aacttcgcac tgcacacctt ccgcggcgcg
    25861 gcgtactcca cgcctgacga gaagtacgag aaatacaagt tcgataccat tgccgataac
    25921 gaaaacctga acatctcttc gaaaggtggt tgggtggcaa tgctgcaaca gtatttcgcg
    25981 acggcgtgga tcccgcataa cgacggtacc aacaacttct ataccgctaa tctgggtaac
    26041 ggcatcgccg ctatcggcta taaatctcag ccggtactgg ttcagcctgg tcagactggc
    26101 gcgatgaaca gcaccctgtg ggttggcccg gaaatccagg acaaaatggc agctgttgct
    26161 ccgcacctgg atctgaccgt tgattacggt tggttgtggt tcatctctca gccgctgttc
    26221 aaactgctga aatggatcca tagctttgtg ggtaactggg gcttctccat tatcatcatc
    26281 acctttatcg ttcgtggcat catgtacccg ctgaccaaag cgcagtacac ctccatggcg
    26341 aagatgcgta tgctgcagcc gaagattcag gcaatgcgtg agcgtctggg cgatgacaaa
    26401 cagcgtatca gccaggaaat gatggcgctg tacaaagctg agaaggttaa cccgctgggc
    26461 ggctgcttcc cgctgctgat ccagatgcca atcttcctgg cgttgtacta catgctgatg
    26521 ggttccgttg aactgcgtca ggcaccgttt gcactgtgga tccacgacct gtcggcacag
    26581 gacccgtact acatcctgcc gatcctgatg ggcgtaacga tgttcttcat tcagaagatg
    26641 tcgccgacca cagtgaccga cccgatgcag cagaagatca tgacctttat gccggtcatc
    26701 ttcaccgtgt tcttcctgtg gttcccgtca ggtctggtgc tgtactatat cgtcagcaac
    26761 ctggtaacca ttattcagca gcagctgatt taccgtggtc tggaaaaacg tggcctgcat
    26821 agccgcgaga agaaaaaatc ctgattcggt gagttttcgc taaaataagg gcggtcagtt
    26881 gaccgccttt tttcttttcg tagggtggat aagcaccgcg catccgccac acaaagcaac
    26941 aggaacatca tgagcgataa tgacactatc gtagcccagg ccacgcctcc gggacgtggc
    27001 ggcgttggca tcctgcgcat ctccggccta aaagcccgtg aagttgccga aaccgtgctg
    27061 ggtaaactgc ctaagccgcg ctacgccgat tatcttccgt ttaaagacgc cgacggcagc
    27121 gtgctcgatc aggggattgc gctatggttc cctggcccga actcgttcac cggcgaagat
    27181 gtgctggaac tgcagggtca tggcggtccg gtgatcctcg acctgctgtt aaaacgcatt
    27241 ctgaccattc ccggcctgcg gattgctcgc cctggtgagt tttccgaacg cgcgtttctt
    27301 aacgataaac tcgacttagc ccaggccgag gcgattgccg atcttatcga cgccagttcg
    27361 gaacaggcgg cccgttcggc acttaactcg ctgcaaggcg cattctccgc acgggtcaac
    27421 catctggtgg aagccctcac ccacttgcgc atttacgtcg aagcggcaat tgatttccct
    27481 gatgaagaga tcgatttcct ctccgacggc aaaattgaag cccagcttaa tgacgtgatt
    27541 gccgatctcg atgcagtgcg tgctgaagca cgtcagggta gtttgttgcg cgaagggatg
    27601 aaggtggtga ttgccggacg tcctaacgcc ggtaaatcga gcctgttaaa cgcgctggcg
    27661 ggccgtgaag cagcaatcgt aactgatatc gccggaacca cacgtgacgt gctgcgtgag
    27721 catatccaca ttgacggaat gccgctgcat atcatcgata ccgctgggct acgtgaagcc
    27781 agtgacgaag tggaacgtat tggtatcgag cgcgcgtggc aggaaattga acaggccgac
    27841 cgcgtgctgt ttatggtcga tggcaccaca acagacgccg tggatccggc agagatctgg
    27901 ccggaattta tcgcccgtct gccagcgaaa ctaccgatca ccgtggtgcg caataaagcc
    27961 gatatcaccg gcgaaacgct gggaatgagt gaagtgaacg gtcacgcgtt aattcgtctc
    28021 tcggcaagga caggtgaagg cgtggaggtg ctgcgtaacc atctcaaaca gagcatgggc
    28081 tttgacacca acatggaagg cggcttcctg gcgcgtcgtc gccacctaca ggcgctggaa
    28141 caggcagcgg agcatttgca gcaaggtaaa gcgcaactgt taggtgcctg ggcgggtgaa
    28201 ctgctggcgg aagagttgcg cctggcacag cagaacttaa gcgaaatcac cggggaattt
    28261 acctctgatg atttgctggg gcggattttc tccagcttct gtattggtaa gtaaccgcgc
    28321 ttacgaagcc gtattctgac tgtcagatgc ggcttcgctt cattgttagc actcctgtta
    28381 ttcctcaacc ctttttttaa acattaaaat tcttacgtaa tttataatct ttaaaaaaag
    28441 catttaatat tgctccc
//
LOCUS       DAESHI010000047        27658 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.047, whole genome shotgun sequence.
ACCESSION   DAESHI010000047 DAESHI010000000
VERSION     DAESHI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27658
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.047"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..807
                     /locus_tag="KW011_002116"
     CDS             <1..807
                     /locus_tag="KW011_002116"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBH7660443.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICAGRPDEGIPAVCFKLKEGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            963..2498
                     /gene="gadC"
                     /locus_tag="KW011_002117"
     CDS             963..2498
                     /gene="gadC"
                     /locus_tag="KW011_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HBH7660444.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2629..3948
                     /locus_tag="KW011_002118"
     CDS             2629..3948
                     /locus_tag="KW011_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HBH7660445.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4320..5702
                     /gene="dosC"
                     /locus_tag="KW011_002119"
     CDS             4320..5702
                     /gene="dosC"
                     /locus_tag="KW011_002119"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HBH7660446.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5727..8126
                     /gene="dosP"
                     /locus_tag="KW011_002120"
                     /gene_synonym="pdeO"
     CDS             5727..8126
                     /gene="dosP"
                     /locus_tag="KW011_002120"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HBH7660447.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            8385..8966
                     /gene="ddpX"
                     /locus_tag="KW011_002121"
     CDS             8385..8966
                     /gene="ddpX"
                     /locus_tag="KW011_002121"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HBH7660448.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8980..10530
                     /locus_tag="KW011_002122"
     CDS             8980..10530
                     /locus_tag="KW011_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH7660449.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10532..11554
                     /locus_tag="KW011_002123"
     CDS             10532..11554
                     /locus_tag="KW011_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH7660450.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11551..12447
                     /locus_tag="KW011_002124"
     CDS             11551..12447
                     /locus_tag="KW011_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HBH7660451.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12444..13430
                     /locus_tag="KW011_002125"
     CDS             12444..13430
                     /locus_tag="KW011_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH7660452.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            13459..14355
                     /locus_tag="KW011_002126"
     CDS             13459..14355
                     /locus_tag="KW011_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH7660453.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIAIARALSSQPDVIVLDEPT
                     SALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQ
                     QVLTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHG
                     CEVRQSLAIREDGRELRCWRAL"
     gene            complement(14411..14842)
                     /gene="osmC"
                     /locus_tag="KW011_002127"
     CDS             complement(14411..14842)
                     /gene="osmC"
                     /locus_tag="KW011_002127"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HBH7660454.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            15187..15402
                     /gene="bdm"
                     /locus_tag="KW011_002128"
     CDS             15187..15402
                     /gene="bdm"
                     /locus_tag="KW011_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HBH7660455.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEQGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            15504..15641
                     /gene="sra"
                     /locus_tag="KW011_002129"
     CDS             15504..15641
                     /gene="sra"
                     /locus_tag="KW011_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HBH7660456.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15798..17495
                     /gene="maeA"
                     /locus_tag="KW011_002130"
     CDS             15798..17495
                     /gene="maeA"
                     /locus_tag="KW011_002130"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBH7660457.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            17629..18631
                     /gene="adhP"
                     /locus_tag="KW011_002131"
                     /pseudo
     CDS             17629..18631
                     /gene="adhP"
                     /locus_tag="KW011_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            18777..19061
                     /locus_tag="KW011_002132"
     CDS             18777..19061
                     /locus_tag="KW011_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBH7660458.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(19289..19942)
                     /gene="fdnI"
                     /locus_tag="KW011_002133"
     CDS             complement(19289..19942)
                     /gene="fdnI"
                     /locus_tag="KW011_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HBH7660459.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(19935..20819)
                     /gene="fdnH"
                     /locus_tag="KW011_002134"
     CDS             complement(19935..20819)
                     /gene="fdnH"
                     /locus_tag="KW011_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HBH7660460.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(20832..23879)
                     /gene="fdnG"
                     /locus_tag="KW011_002135"
     CDS             complement(20832..23879)
                     /gene="fdnG"
                     /locus_tag="KW011_002135"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(23292..23294),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBH7660461.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            24111..24992
                     /gene="yddG"
                     /locus_tag="KW011_002136"
     CDS             24111..24992
                     /gene="yddG"
                     /locus_tag="KW011_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HBH7660462.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            25251..26351
                     /gene="ompC"
                     /locus_tag="KW011_002137"
     CDS             25251..26351
                     /gene="ompC"
                     /locus_tag="KW011_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBH7660463.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            26515..26997
                     /locus_tag="KW011_002138"
                     /pseudo
     CDS             26515..26997
                     /locus_tag="KW011_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27112..>27658
                     /locus_tag="KW011_002139"
     CDS             27112..>27658
                     /locus_tag="KW011_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBH7660464.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAH"
BASE COUNT         6972 a         6895 c         6822 g         6969 t
ORIGIN      
        1 gacgaaaata ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccgcaac cgctgcacga tgcactggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgccgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca caaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctcggtggt cagattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtgat tgcacagtac tatgaattcc tgcgccttgg tcgtgaaggc
      421 tataccaaag tacagaatgc ttcctaccag gtcgctgcct atctggcgga tgagatcgcc
      481 aaactggggc cgtatgagtt catctgtgcc ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaagaggg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
      601 ctgcgtctgc gcggctggca ggttccggcc ttcactctcg gcggtgaagc caccgacatc
      661 gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg actttgctga actgttgctg
      721 gaagactaca aagcctccct gaaatatctc agcgatcacc cgaaactgca gggtattgcc
      781 cagcagaaca gctttaaaca tacctgataa cgtttaacgg taacggtgtc ccgaaacgaa
      841 cccgtttcgg gataatttcc aaagtctgtt cactggcatt agcaacggaa aatattgttc
      901 tgaatacgct tcagaacaaa acaggtgcgg ttccgacagg aataccgttt tagggggata
      961 atatggctac atcagtacag acaggtaaag ctaagcagct cacattactc ggcttttttg
     1021 ccataacggc atcgatggta atggctgttt atgaataccc taccttcgca acatcgggct
     1081 tttcattagt cttcttcctg ctattaggcg ggattttatg gtttattccc gtgggacttt
     1141 gtgctgcgga aatggccacc gtcgacggct gggaagaagg tggtgtcttc gcctgggtat
     1201 caaatactct ggggccgaga tggggatttg cagcgatctc atttggctat ctgcaaatcg
     1261 ccattggttt tattccgatg ctctatttcg tgttaggggc actctcctac atcctgaaat
     1321 ggccagcgct gaatgaagac cccattacca aaactattgc agcactcatc attctttggg
     1381 cgctggcatt aacgcagttt ggtggcacga aatacacggc acgaattgct aaagttggct
     1441 tcttcgccgg tatcctgtta cctgcattta ttttgatcgc attagcggct atttatctgc
     1501 actccggtgc ccccgttgct atcgaaatgg attcaaagac cttcttccct gacttctcta
     1561 aagtgggcac cctggttgta tttgttgcct tcattttgag ttatatgggc gtagaagcat
     1621 ccgcaaccca cgtcaatgaa atgagcaacc cagggcgcga ctatccgttg gctatgttac
     1681 tgctgatggt agcggcaatc tgcttaagct ctgttggtgg tttgtctatt gcgatggtca
     1741 ttccgggtaa tgaaatcaac ctctccgcag gggtaatgca aacctttacc gttctgatgt
     1801 cccatgtggc accggaaatt gagtggacgg ttcgcgtgat ctccgcactg ctgttgctgg
     1861 gtgttctggc ggaaatcgcc tcctggattg ttggtccttc tcgcgggatg tatgtcacag
     1921 cgcagaaaaa cctgctgcca gcggcattcg ctaaaatgaa caaaaatggc gtaccggtaa
     1981 cgctggtcat ttcacagctg gtgattactt ctatcgcgtt gatcatcctc accaataccg
     2041 gtggcggtaa caacatgtcc ttcctgatcg cactggcgct gacggtggtg atttatctgt
     2101 gtgcttattt catgctgttt attggctaca ttgtgttggt tcttaaacat cctgacttaa
     2161 aacgcacatt taatatccct ggtggtaaag gggtgaaact ggtcgtggct attgtcggtc
     2221 tgctgacttc aattatggcg tttattgttt ccttcctgcc gccggataac atccagggtg
     2281 actctaccga tatgtatgtt gaattactgg tcgttagttt cctggtggta cttgccctgc
     2341 cctttattct ctatgctgtt catgatcgta aaggcaaagc gaataccggc gtcactctgg
     2401 agccaatcaa cagtcagaac gcaccaaaag gtcacttctt cctgcacccg cgtgcacgtt
     2461 caccacacta tattgtgatg aatgacaaga aacactaacc atgtctgaat ggttataaga
     2521 caagggagcg ataattcatc gctccctttt tcgtgctcgc tgtcgtattg acttaaccgg
     2581 gataagtacg agaatgagcg cacatctgtt taccggaaac cagcatatat ggatatctgc
     2641 tcccgaaacg agaaattagc gattagaaga ccagcgatac tggttgcact ggcactttta
     2701 ctgtgtagtt gtaaaagcac gcctccagag tccatggtga caccaccagc aggttcaaag
     2761 ccaccagcca cgacgcaaca atcgtcacaa ccgatgcgtg gcatctggct ggccacggtg
     2821 tctcgcctcg actggccacc ggtttcctcg gttaacatta gtaaccccac cagccgggcc
     2881 cgtgtacaac aacaggcgat gatcgacaaa ctggatcatc tgcaacgtct cggtataaac
     2941 acggtctttt tccaggttaa gccggacggt accgccctgt ggccatcgaa aattttgcca
     3001 tggtccgatc ttatgaccgg taagattggt gaaaatccgg gttacgatcc gctgcaattt
     3061 atgctcgacg aagcccacaa gcgtgggatg aaagtacacg cctggtttaa cccctatcgc
     3121 gtatcggtta atacgaagcc cggtactatc agggaactga atagcactct gtctcaacaa
     3181 ccggcgagcg tctatgtgca acaccgtgac tggatcagaa cgtctggcga tcgctttgtc
     3241 ctcgacccgg gcatccctga ggttcaggac tggatcacat caatagtcgc agaagtggtt
     3301 tcccgctatc cggtagatgg cgtgcagttt gacgactatt tctatactga gtcaccgggt
     3361 tcacggctaa atgataacga aacgtaccgc aaatacggag gcgcatttgc gtcaaaagca
     3421 gactggcggc gcaacaatac tcagcagtta attgcaaaag tatcgcacac cattaaaagc
     3481 attaagccgg gagtcgaatt tggcgttagc ccggcaggcg tgtggcgtaa ccgatcacac
     3541 gatccgctcg gttccgatac ccgaggcgcg gcagcctatg acgaatccta cgctgacacc
     3601 cgtcgatggg tggaacaagg attgctggat tacattgctc cccaaattta ctggccgttc
     3661 tcacggagtg ccgcgcgtta tgacgtgttg gcaaaatggt gggcggatgt cgttaaaccg
     3721 accaggaccc gcctgtatat cggtatcgcc ttctataaag tgggtgaacc ttcaaagata
     3781 gagccagact ggatgattaa cggcggcgta ccggaactga aaaagcagct cgatcttaac
     3841 gatgcggtac cagaaattag cggcaccatc ttgttccgtg aggactatct gaataaaccg
     3901 cagactcaac aagcggtcag ctatctgcaa agtcgttggg gcagttaaac tcccatgctt
     3961 actttgtaac aagccggtgc tttacccacc ggcttgttac accttgtgaa atatcccact
     4021 agtaatcatg cttacataag tcaaattaac cacgcaatta accacactta gttaaacagc
     4081 tataaatccc taataaaaat gtatgaatat gaaaaaccat acgatattcg ctgtcaccga
     4141 aaaataatta cgatgggcta tactcttctg aagaacagta gcgtagattg aaactgtcct
     4201 aagtcgctta tttcttttca gtatatcttc atatttcagg agaatatggt gcagttagtc
     4261 aatctcgcac gttgcgtttt gattccgtct gatttcccag ccttataagg gtgtgaatta
     4321 tggagatgta ttttaaaaga atgaaagatg agtggaccgg acttgtcgaa caggcagatc
     4381 cactcattcg tgctaaagcc gcggaaattg ccgttgcgca tgctcattat ctgagtatcg
     4441 agttttatcg aattgtccgc atcgacccgc atgccgaaga attcttgagt aatgaacaag
     4501 ttgagcggca gttgaaaagt gcgatggaac gctggattat taacgtgctt tctgcccagg
     4561 ttgacgatgt cgaaaggcta atacaaatcc agcataccgt cgcggaagtg catgcccgca
     4621 taggaattcc ggtagaaatt gtcgagatgg ggtttcgggt gctgaaaaaa atcctctatc
     4681 cggtaatctt ctcttcggat tattccgccg cagaaaaact tcaggtctat catttctcga
     4741 ttaacagtat tgatatcgcg atggaagtga tgacccgcgc gtttaccttt agtgacagta
     4801 gtgcctcaaa ggaagatgaa aattatcgta tcttctcgtt actggaaaac gccgaagaag
     4861 aaaaagaacg gcaaatagcc tcaatacttt catgggaaat agatattatc tataaaatcc
     4921 tgctggattc tgatttaggc agtagtttgc cattaagcca ggctgatttt ggcctgtggt
     4981 ttaaccataa aggtcgacat tattttagtg gcattgccga agtaggccat atctcccgtc
     5041 tcattcagga tttcgacggt attttcaatc aaaccatgcg taacaccagg aatttgaata
     5101 acagaagtct gcgggtgaaa tttttattac agataagaaa taccgtatcg cagattatta
     5161 ccttgctgcg tgaattgttt gaagaagtat cgcgccacga agtcggtatg gatgtactaa
     5221 cgaaattact taaccgccgt ttcctgccga ctatcttcaa acgcgaaatt gcccatgcca
     5281 accggaccgg tacaccgctg tcagtgctga ttattgacgt tgataaattc aaagagatca
     5341 acgatacgtg gggccataac actggcgatg aaattctgcg taaagtctct caggcctttt
     5401 atgacaacgt ccacagtagt gattatgttt ttcgctacgg gggcgatgaa tttatcattg
     5461 ttttgactga agcttctgaa aacgaaacgt tacgtaccgc agaacgtatt cgcagtcggg
     5521 tggagaaaac caaactgaaa gccgcaaacg gcgaggatat tgccctctca ctttccatcg
     5581 gtgccgccat gtttaatggt catcctgact atgagcgcct cattcaaata gccgatgaag
     5641 ctctgtatat cgccaaaaga cgaggtagaa accgtgttga actctggaaa gccagtcttt
     5701 agatgcgcca ggatgcagag gtaatcatga agctaaccga tgcggaaaat gccgccgatg
     5761 gcattttttt ccccgccctt gaacaaaata tgatgggcgc ggtgttaatt aacgaaaatg
     5821 atgaagtgat gtttttcaac cccgccgcag agaagctctg ggggtacaaa cgtgaagaag
     5881 tcattggcaa taacattgat atgctgattc cgcgggattt gcgtcctgcg catcctgaat
     5941 acattcgtca taaccgtgaa ggtggtaaag cgcgcgtcga ggggatgagt cgggagctgc
     6001 agctggagaa aaaagacggc agtaaaatct ggacccgttt tgcgctatcg aaagtgagcg
     6061 ccgaggggaa agtttattac ctggcgctgg tacgggatgc cagcgtagaa atggcgcaaa
     6121 aagaacagac ccgacaattg attattgccg ttgaccatct cgaccgaccg gtgattgtcc
     6181 tcgatccgga acgccatatt gtgcagtgca atcgcgcatt taccgaaatg tttggttact
     6241 gcattagcga agccagcggt atgcagcccg atacactcct gaacattcct gaattccctg
     6301 ccgataaccg cattcgttta caacagttgc tatggaaaac cgcccgcgat caggacgaat
     6361 ttctgctgtt gacacgcacc ggtgaaaaaa tctggattaa agcctctatc agcccggttt
     6421 atgacgtgct cgcgcatctg cagaacctgg taatgacctt ctcggatatc accgaagaac
     6481 ggcagatccg ccagcttgaa ggcaatattc tcgccgccat gtgcagcagc ccgccatttc
     6541 atgaaatggg ggaaatcatt tgtcgtaaca tcgaatctgt tctcaacgaa tcgcatgttt
     6601 cgctgttcgc actgcgcaac gggatgccga tacactgggc gtcatcttcg cacggtgcag
     6661 aagttcaaaa tgcgcaaagc tggtcagcga ccattcgtca gcgtgatggt gcgcctgcgg
     6721 ggatcctgca aattaaaacc tcgtcaggag cagaaaccag cgcctttatc gaacgcgtgg
     6781 cagatatcag ccagcatatg gccgcgctgg cgctggaaca ggaaaaaagc cgtcagcata
     6841 ttgaacaact catccaattt gatccgatga ccggtctgcc aaatcgcaat aacctgcaca
     6901 attacctcga tgaccttgtc gacaaagccg tctctccggt ggtgtatctc atcggtgttg
     6961 accatattca ggatgtgatt gatagccttg gctatgcgtg ggccgatcag gcattgctgg
     7021 aagtggtcaa tcgctttcgt gaaaaactca aaccggatca gtatctctgt cgtatcgaag
     7081 gtacgcagtt tgtcctcgtg agcctggaaa acgacgtcag taacattacc caaatcgccg
     7141 atgagctacg gaatgtggtc agcaagccga taatgattga cgataaacca ttcccgctaa
     7201 ccttgagtat tggcatcagc tacgacgtgg gtaaaaaccg cgattacctg ctctccactg
     7261 ctcacaatgc aatggattat attcgcaaga atggcggtaa cggctggcag ttcttcagcc
     7321 cggcgatgaa cgaaatggta aaagagcgtt tggttttagg cgcagcgctg aaagaagcga
     7381 ttagcaataa ccaactgaaa ctggtttacc agccgcaaat cttcgcagaa acgggtgaac
     7441 tgtacggcat cgaagccctt gctcgctggc acgatcccca gcatggtcat gtaccccctt
     7501 cacggtttat tcctctcgca gaagagattg gtgaaatcga aaatattggg cgctgggtca
     7561 tcgcggaagc ttgccgtcag ttagcagaat ggcgtagcca gaatattcat atcccggcgt
     7621 tatccgtgaa cttgtcggcg ctgcactttc gcagtaatca gctgcctaat caggtgtctg
     7681 atgcaatgca agcctggggt attgacggcc accagctgac ggtagaaatc acggaaagca
     7741 tgatgatgga acacgatacc gaaatcttta agcgcattca gatcctgcgc gatatgggcg
     7801 tgggcttatc ggtagatgat tttggcacgg gcttttccgg attatcccgc ttagtcagtc
     7861 ttccggtaac ggaaatcaaa attgacaaaa gttttgtcga tcgttgtctg acggaaaaac
     7921 gcatccttgc cttacttgaa gccattacca gcattgggca aagcctcaat ttaaccgtcg
     7981 tggcggaagg cgtcgaaacc aaagagcaat ttgagatgct acgcaagatc cactgtcgcg
     8041 ttattcaggg atatttcttt tcccgccccc tacccgccga ggaaattcca ggctggatga
     8101 gcagcgtgtt accgctgaaa atctgacaaa ttcctctcgc ccgcactcgc gggttttcat
     8161 ttaacgtgac actgtcactt aaatactgtg atttcagcta cgattccggg agattccttc
     8221 cttaacttcc cctcttgcac caaaactgca ctacgctgca caaatgttga tcaaaagtta
     8281 aaaaatataa ctttaagcca gcattctggc gcttatcccg gcatggcatg agatctgcat
     8341 aagcggaaag cgcagcaatt tttgtcttaa tacaggaacc gtttatgtcg gataccaccg
     8401 aactggttga tttagccgtg atcttccctg atctggagat cgaattgaaa tacgcctgcg
     8461 ctgataacat cacaggtaaa gctatttacc agcaagcgcg ttgtctgtta cacaaggatg
     8521 cgattaccgc gctggcgaaa agtatcagta tcgcccagct gtcaggctta caactggtga
     8581 tttacgatgc gtatcgccca caacaagcac aggcgatgtt gtggcaagcc tgcccagacc
     8641 cgcaatatgt tgttgatgtg acggtcggtt ctaatcacag ccgtggcacg gcgatcgacc
     8701 tgacgcttcg tgatgagcac gggaacatcc tggatatggg cgcagggttc gatgaaatgc
     8761 acgagcgctc ccatgcctat cacccttccg tcccgcccgc cgctcagcgc aatcgcctgt
     8821 tgctgaatgc gataatgact ggtggcggtt ttgtcggtat ctccagcgaa tggtggcact
     8881 tcgaattacc ccaggcagcg agttaccctc tgctcgctga tcaattctct tgttttatat
     8941 cccctggcac acagcacgtc agttaatcag gaaactgtca tgaagagatc gatatcgttt
     9001 cgtcccacat tgctcgcgct cgtccttgcc acaactttcc cggttgcgca cgccgccgta
     9061 ccgaaagata tgctggtgat tggtaaggcc gccgatccac aaaccctcga cccggcggta
     9121 acaatagata ataacgactg gacagtgacc tacccgtctt atcagcggct ggttcagtac
     9181 aaaacggacg gtgataaagg ctcaaccgac gttgaaggcg atctggcaag tagctggaaa
     9241 gcgtctgacg atcaaaaaga gtggacgttc accctgaagg acaatgctaa atttgccgat
     9301 ggcacacctg tcactgccga agcagtaaaa ctctcttttg agcggttact aaaaatcggc
     9361 caggggccag cagaagcatt tcccaaagat ttaaagattg atactcccga cgaacatacg
     9421 gtgaagttta cccttagcca accattcgca ccgttcctct acacgctagc gaatgacggt
     9481 gcatccatta tcaatccggc ggtcttaaag gaacatgcag cggatgatgc tcgcggcttc
     9541 ctcgcgcaaa ataccgccgg ttccggacca tttatgctga aaagctggca aaaaggtcag
     9601 caattagttc tggtgccaaa tccgcattac cccggcaata aaccgaactt taagcgagta
     9661 tcggtaaaaa ttatcggtga aagtgcctcc cgtcgcctgc agctctcccg tggcgacatt
     9721 gacattgccg atgcgctgcc ggtggatcaa ctcaacgccc tgaagcagga aaataaagtc
     9781 aatgtggcag agtatccgtc actgcgcgtt acctatctgt atctgaataa cagcaaagcg
     9841 cctcttaatc aggcggatct gcgtcgggcc atttcctggt ctaccgatta tcagggcatg
     9901 gttaacggca ttctgagtgg taacggaaaa cagatgcgcg gcccgattcc ggaaggcatg
     9961 tggggctacg atgcgacggc aatgcaatac aaccatgacg aaacgaaagc caaagccgaa
    10021 tgggataaag tgacgagcaa acccaccagc ctgacgtttc tctactccga taacgatccg
    10081 aactgggagc ctattgctct ggcgacacaa tccagtctca acaagctggg catcaatgtg
    10141 aagctggaaa agctggcgaa cgccaccatg cgcgacagag tgggtaaagg tgattacgac
    10201 attgcgattg gcaactggag tccggatttt gccgacccgt atatgtttat gaattactgg
    10261 tttgagtcag acaaaaaagg tctgccgggt aaccgctcgt tctatgaaaa cagtgaggtc
    10321 gataagttac tgcgcaatgc gcttgcgacc accgaccaga cgcagcgtac ccgggactac
    10381 cagcaggcac agaaaatcgt cattgatgac gctgcttatg tgtacctgtt ccagaaaaac
    10441 taccaactgg cgatgaacaa agaggtgaaa ggctttgtgt tcaatcccat gctggaacag
    10501 gtcttcaata tcaataccat gagtaaataa cgtgaccttc tggagtattt tacgccaacg
    10561 ctgctggggg ctggtgctcg tggtggcggg cgtctgcgtg attacgttta ttatctcgca
    10621 tctgatccct ggcgatccgg cgcggttact ggcgggtgac cgcgccagcg atgctatcgt
    10681 ggaaaatatt cgccagcaac tgggactgga ccagccactg tatgtacagt tttaccgcta
    10741 cttcagcgac ctgtttcatg gtgacctggg aacatccatt cgtaccgggc gtccggtgct
    10801 ggaagagttg cgtatatttt tcccggcgac gctggaactg gctttttgtg ccctgctgct
    10861 ggcactcctg attggcatcc cgctgggcat actctctgca gtctggcgaa atcgctggct
    10921 ggatcatctg gtgcgaataa tggccattac cggaatctcc acacctgcgt tctggcttgg
    10981 actgggcgtc attgtgctgt tttatggtca tctgcaaatt cttcccggcg gcggaaggct
    11041 tgatgactgg ctggatccac caacgcacgt taccggcttt tatctgctcg atgcgctgct
    11101 tgaaggcaac ggtgaagtct tcttcaatgc gttgcaacat ctcatcttac cggcattaac
    11161 gctggcgttc gttcacctgg gaattgtcgc tcgccagatc cgctcagcga tgctggaaca
    11221 attgagtgaa gactacattc gtaccgcccg ggccagcggc ttgcccggct ggtatatcgt
    11281 tttatgttat gcgctaccca atgcgttgat cccatcgatt accgtattgg gtctggcgct
    11341 gggcgatttg ttgtatggcg cagtgctcac cgaaaccgtt tttgcctggc ccggaatggg
    11401 tgcatgggta gtaacatcaa tacaggcgct cgacttcccg gcagtgatgg gctttgccgt
    11461 cgtggtttca tttgcttatg tgctggtcaa cctggtggtg gatttgctct atttgtggat
    11521 tgatccgcgt atcggacgtg gaggtggtga atgatgctaa gcgaggaaac gtccgccgta
    11581 cgcccacaaa aacaaacgcg atttaacggt gcaaaactgg tatggatgct gaaaggcagt
    11641 ccgctcaccg tgaccggcgc agtcatcatt gtattaatgc tattgatgat gattttttca
    11701 ccgtggcttg cgacgcatga tcccaacgcc attgatttaa ccgcccgcct tttgccgcct
    11761 tctgcggcgc actggtttgg caccgatgaa gtgggacgcg atctgttcag ccgcgtactg
    11821 gtcggcagtc agcaatcaat tctcgccgga ttagtcgtgg ttgccattgc gggtatgatt
    11881 ggttcgctac tcggatgtct atccggtgtg cttggcggac gcgcagacgc catcatcatg
    11941 cgcatcatgg acattatgct gtcgattcct tcgttggtac tgacaatggc actggctgcc
    12001 gctctcgggc cgagtttatt taacgccatg ctggcgattg ctattgtgcg aattcccttt
    12061 tatgtgcgcc tggcgcgggg gcaagcatta gttgtacgcc agtataccta tgttcaggcg
    12121 gcgaaaacct ttggtgcgtc gcgttggcat ctgatcagct ggcatatttt acgtaactcc
    12181 ctgccgccgc tgatcgtgca ggcatcgctg gatatcggta gcgcgatttt aatggccgcc
    12241 acgttgggat ttattggcct gggtgctcaa caaccgagtg ctgaatgggg cgcgatggta
    12301 gctaatggtc gcaactatgt actcgatcag tggtggtatt gcgcatttcc gggggcagcg
    12361 attttgctta ccgccgtcgg gtttaatctc tttggtgatg gtattcgcga tctgcttgac
    12421 ccgaaagcag gaggaaagca gtcatgaccc aacccgttct ggacattcaa cagctgcatt
    12481 tgagtttccc cggttttaac ggttatgttc acgtgctcaa caatgtgtcc ttgcagatta
    12541 accgcggtga aattgtcggt ctggtgggag aatccggctc aggtaaatca gtcaccgcaa
    12601 tgctgattat gcgtctgcta ccgacgggca gttattgcgt acatcggggg cagatttcac
    12661 tgctgggaga cgatgttctt aacgctcggg agaaacagct tcgtcagtgg cgcggcgcac
    12721 gagtggcgat gatctttcag gaaccgatga ccgccctcaa tccgacacgt cgaataggtc
    12781 ttcagatgat ggacgtgatc cgccatcatc aaccaataag tcgtcgggaa gccagagcta
    12841 aagcgattac cctgctggaa gagatgcaaa tcccggatgc cgtggaagtt atgtcgcgct
    12901 atccgtttga gctttcaggt ggtatgcgcc agcgggtaat gattgcgctg gcattctcct
    12961 gcgagccgca attgattatt gccgacgaac cgactacggc gctggacgtc acggtacagt
    13021 tgcaggtact gcgtttgctt aaacataaag ctcgcgccag tggaactgcg gtactgttca
    13081 tcagccatga tatggccgtg gtgtcgcaac tgtgcgatag cgtttacgtg atgtatgccg
    13141 gaagcgtaat tgaaagcggc gtgacggcag acgttatcca tcatccccgg catccgtata
    13201 ccattggttt gctgcaatgc gcaccagaac atggagtacc acgccagcca ttacccgcca
    13261 ttccagggac ggtaccaaac ctcacccatt tgcctgacgg ctgcgctttt cgcgatcgtt
    13321 gctatgcggc aggtgcacag tgtgaaaacg tcccggcgct gacagcgtgt ggtgacaaca
    13381 accagcgctg cgcctgttgg tatccgcagc aggaggtcat aagtatctga cacgttatta
    13441 acgttacgcg acgtccatat caatttcccg gcccgtaaaa gctggcttgg taaaactacg
    13501 gaacatgttc atgcgattaa tggtattgat ttacagatcc gccgtggtga aaccttaggg
    13561 atcgtcggtg agtcaggctg cggcaaaagc accctcgcac agcttttaat gggtatgctg
    13621 caaccgagcc acgggcaata catccgttca ggatcacaac gcattatgca gatggtgttt
    13681 caggacccgc tctcttcgct taatccgcgc ttatcggtgt ggcgcatcat cacagaaccg
    13741 ctctggatag ctaagcgtag tagtgaacaa cagcggcgag cgttggcaaa ggagttggct
    13801 gtgcaggtgg gtattcgtcc ggagtatctc gaccgcctgc ctcatgcgtt ctccggcggg
    13861 cagcggcaac gcatcgccat tgccattgcc agagcactct cttcgcagcc tgacgtgatt
    13921 gtgcttgatg agccaacctc ggcgctggat atctccgtgc aggcgcagat cctcaattta
    13981 ctggtaacgc tacaggaaaa tcacgggctg acttatgtgc tgatttcaca caatgtctcg
    14041 gtgatacgtc atatgagcga tcgggtggcg gtgatgtatc tcgggcagat tgtagaactg
    14101 ggcgacgcgc agcaggtgct gacggcacct gcacatccat acacccgatt attgctggat
    14161 tccctccccg ccattgataa accgctggag gaagaatggg cattatgtaa aacggatctg
    14221 ccgggaaacc gcacgttgcc acaaggctgt tttttctacg aacgttgccc tctggcaacc
    14281 cacggatgtg aagtccggca atcattagca ataagggagg acggacgtga gctccggtgc
    14341 tggcgggcgc tgtagagatg ctaacaacgc atcaggcatt cacgcgcctg acgcgtcatc
    14401 cggcaatgct ttacgatttc aactggtaat ccagcgtaat ttccgctttc agtacctgag
    14461 aaaccgggca tcctgctttt gctttctgga tgatgccgtc aaaggtagag gcatcaatac
    14521 ccggcaccgc aacttcactc ttcagtgcga ttttcgtaat cgcaaaatcg gcatccactt
    14581 tatccagcga cacatcggcg gtggtatcaa tcgatgttgg cgtgaatccc gcttccccca
    14641 gcattaatga aagcgccatt gagaaacatg cggcatgcgc tgcgccaatc agttcttcag
    14701 ggttggttcc tttttcgcct tcaaaacgcg tgttaaatcc atacggctgt tggttcagca
    14761 cgccactctg ggtggatact gttcccttcc cgcgtttgat atcgccttcc cagtgtgcct
    14821 gacctttctt atggattgtc attgttgctc tcctgtgggc ttaaaaagga aattatagac
    14881 tacgtgagaa acagcacgag gataagcagg atattccgaa taaaatacgg cgaaaatatt
    14941 acttctgcac ttaaattaaa ttcccacaat agataaccgg gaatatatat ctcaagagat
    15001 attgcatcaa tatcaatcaa ctcaccacaa taataataca tatcaatatc atggagttaa
    15061 aatataaaaa tcatcaacca ggactaatct taacaacgaa gcggcaaata tttgccatgc
    15121 tgaatgtgct ttataaatct gcgatccgta gcagacacca taaatacaca gacacggaga
    15181 atcactatgt ttacttatta tcaggcagaa aattcaacag cagaacccgc tctggttaat
    15241 gccattgagc aaggccttcg ggcagagcaa ggtgttgtca ctgaagatga cattctcatg
    15301 gagctgacca aatgggtgga agcttctgat aacgacatcc tcagtgatat ctaccagcaa
    15361 acgatcaatt atgtggtcag tggccagcac cctacgcttt aaggtgctat gcttgatcgg
    15421 caacctaatt taggggttta gcacgtgttt cttcgctacg gcgatgttgt ccttaaaact
    15481 agcaacagga ttgaggagtt aaaatgaaat cgaaccgtca ggcacgtcat attcttggac
    15541 tggaccataa aatttctaac cagcgcaaaa tagttaccga aggtgacaaa tccagcgtgg
    15601 taaataaccc aaccggcaga aaacgccccg ctgaaaagta attcataacc atcagtcctc
    15661 aatgacgatt aaacaccatt gcctgcgcaa tggtgttttt gtttttatct gctttatact
    15721 tggggccgac gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc
    15781 aaaaaagagt gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt
    15841 acgctggccc tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg
    15901 aagaacgccg taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag
    15961 aacaagcgga acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca
    16021 tctacctgcg taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc
    16081 atcttgatga gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt
    16141 tttctgagat ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata
    16201 tggacgatat tctgcaaaac gtaccgaacc ataatatcaa agtgattgtg gtgactgacg
    16261 gtgaacgtat tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta
    16321 aactgtcgct ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc
    16381 tggatgtcgg aacgaacaac caacaactgc ttaacgatcc gctgtatatg ggctggcgta
    16441 atccgcgtat cactgacgac gaatactatg aattcgttga tgaatttatc caggctgtga
    16501 aacaacgctg gccagacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc
    16561 cgttacttaa ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg
    16621 cggcggtaac agtcggcaca ctgatcgcag caagccgagc ggcaggtggt cagttaagcg
    16681 agaaaaaaat cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca
    16741 tcgcccagac ccagcgcgaa ggattaagcg aggaagcggc gcggcagaaa gtctttatgg
    16801 tcgatcgctt tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac
    16861 tggtgcagaa acgcgaaaac ctcagtgact gggataccga cagcgatgtg ctgtcactgc
    16921 tggatgtggt gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc
    16981 tgtttacgga agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc
    17041 cgctgtctaa cccgacgtca cgcgtggaag ccacaccgca ggacattatc gcctggaccg
    17101 aaggtaacgc gctggtcgcc acgggcagcc cgtttaatcc agtggtatgg aaagataaaa
    17161 tctaccctat cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggggtta
    17221 ttgcttccgg cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg
    17281 cacagtattc gccgctggtg ctgaacggcg aaggtctggt actaccggaa ctaaaagata
    17341 ttcagaaagt ctcccgtgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg
    17401 tggcggtgaa aacctctgcc gaagctttgc aacaagccat tgacgataat ttctggcaag
    17461 ccgaataccg cgactaccgc cgtacctcca tctaagcctg cgcccggtag tgaaggctac
    17521 cgggcaattt tcctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc
    17581 tcatttgcct cacctgctat gcagaacatc atccgaaaag gaggaactat gaaggctgca
    17641 gttgttacga aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat
    17701 ggcgaagccc tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag
    17761 aatggcgatt ttggtgacaa aaccgacgta attctgggcc atgaaggcat cggtgtggtg
    17821 gcagaagtgg gtccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg
    17881 ttctacgaag gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt
    17941 tcagttaaaa atgccggata cagcgttgat ggcggaagag tgcatcgtgg tcgccgatta
    18001 cgcggtaaaa gtgccagatg gtctggactc ggcggcggcc agcagcatta cctgtgcggg
    18061 ggtcaccacc tacaaagccg ttaagctgtc aaaaattcgt cccgggcagt ggattgctat
    18121 ctacggtctt ggcggtctgg gtaacctcgc cctgcaatac gcaaagaatg tctttaacgc
    18181 caaagtgatc gccattgatg tcaatgatga gcagttaaaa ctggcaaccg aaatgggtgc
    18241 agatttagcg attaactcac gcaccgaaga cgccgccaaa attgtgcagg agaaaaccgg
    18301 tggcgctcac tctgcggtgg taacagcagt agctaaagct gcgtttaact cggcagttga
    18361 tgctgtccgt gcaggcggtc gtgttgtggc tgtcggtctg ccgccggagt ctatgagcct
    18421 ggatatccca cgtcttgtgc tggatggcat tgaagtggtc ggttcgctgg tcggcacgcg
    18481 ccaggatcta accgaagcct tccagtttgc cgccgaaggc aaagtggtgc cgaaagtcgc
    18541 cctgcgtccg ttagcggaca tcaacaccat ctttaccgag atggaagaag gcaaaatccg
    18601 tggccgtatg gtgattgatt tccgtcacta agaggtcttt gctgcgactc ccatgttcgg
    18661 gtcgcagcat cgcacactct ccaacatgaa atggctaaat ggattattgt tgcgtaggaa
    18721 ggaacaataa tgatagtgtt cccagagaag gaacgctact gagagtaaag acactaatga
    18781 aaatggccaa tcatccccgc ccgggggaca ttattcagga atcactggac gaacttaatg
    18841 tcagcctgcg cgagtttgcc agagcaatgg aaattgcgcc ctcaacggca agccgattgc
    18901 tgaccggaaa agcagctttg acgccagaaa tggcaattaa actttccgtg gtgatcggca
    18961 gttcgccgca aatgtggctg aatctgcaaa acgcctggag tctggcagag gcagaaaaaa
    19021 cggtggatgt gtcgcgatta cgccgtctgg ttacgcaata aaaaatggca ccttataaag
    19081 cgccatttga aactgatgac aaacgcaaca ttgcctgatg cgctacgctt atcaggccta
    19141 cacgttcact tcaatctatt gtatttgaag ggttttcatg ccggataagg cattcacgac
    19201 gcatccggga tgaacaaagc ccacgttgtc tacaatatga aaatggcgcc ttttacagcg
    19261 ccattttcag cttaaagttc aaaagagatt atatcccttc ttcactctct tttttcgctt
    19321 ctgccttctc gatttcacga taccagcgcg gatggtgttt cttcgcccag cgacggctca
    19381 ccttcccttc gatcatccct ttaatcgacc ctttcaccca gaatgccata tacatatgga
    19441 tcaggatggc gtggatcagg atgatacccg cagccgcgtg gatcagcagg ctgtagcgaa
    19501 caacctgcat cgggaagtac tgcgcaaagt acggacgcca gataatcacc ccggtcacca
    19561 gcagcacgaa aatcatgctc atgatcgacc agaacatcat cttttgcccg gcgttgtact
    19621 taccaacatc cgccactttg tgctcattgc cttttaatac ttcgacaatg ttcaacagcc
    19681 acggaatatc tttcttatcc gggatgttgt gatgcacaaa acgcacaaac ataaacatca
    19741 gtgcgacgaa aatcgcaatg ccgaagaacg ggtgcaaaat gcgtcccatc tgcggcgtac
    19801 cgaaggtttg cgtcagccat tgcagcgtcg ggaagaagaa cgaaatcccg gacagcgcca
    19861 ccaggaagaa gcaaatcacc acggtccagt gacaggcgcg atcaataaat ttggtgcgca
    19921 caatcatttt cgacttactc atgatgatcc tcctcgtcat cgtccacttc cttattcggg
    19981 ccaataccga tgtagtggaa aatcaacccg gcaaaagtgg caataaagcc agccgctgcc
    20041 agcggtttca acgcgccttt ccacaggctt accgaggtgt cgatcttcgg atctttcggc
    20101 agaccgtaat acagctccgg ctgatcggcg tgatgcagca cgtacataac gtgcgtacca
    20161 ccgacccctt tcgggttgta gacgccagca tgttcgtaac cacgcgcttt cagtttcgcc
    20221 acgcgctctt ccgccagctc cagcatctcc ttcttggtgc cgaagtggat agccccggtc
    20281 ggacaggttt tcacacaagc cggttcctgg ccgacgctga cgcgatcgac gcagagcgtg
    20341 catttatata cccggttatc ctctttgttg aggcgcggaa tattaaacgg acacccggca
    20401 atgcagtaac cacagccgat gcagttttcc gactggaaat cgacaatccc gttagcgtac
    20461 tgaatgattg caccagcaga cgggcacgcc ttcaggcagc cgggatcttc acagtgcata
    20521 cagccgtctt tacggatcag ccactccagc ttgccgttct gttcggtttc gctaaagcgc
    20581 atcaccgtcc aggacttggc gctcagatcg gcggggttat cgtaaacccc gacgcagtgc
    20641 cccacttcat cacggatgtc gttccactcc gaacacgcca cctgacaggc tttacagccg
    20701 atacaggtgg aaacgtcgat aagttttgcg acttctgctt tgtaatcacg cacctgagaa
    20761 ggcggcgtga tggagttagt tgcggacctt ttgataatat cctgcgtttc catagccatc
    20821 tgttcgcccc cttacgcctt ctcgatgttg actaagaacg ctttatattc cggcgtttgc
    20881 gagtttgcat caccgacatt cggcgtcaga gtgttagcga tataaccttt acgcgcgaca
    20941 ccctcaaagc cccagtggat tggaataccc accgtttcaa cctgctgacc gtttacattc
    21001 agcggtttca gacggcgcgt taccaccgcc accgcgcgga taaagccacg cttgcttgag
    21061 acagtgacac gatcgccatt attaatgcct ttcgccgccg ccagcgtttc gctgatttcc
    21121 acaaactgtt ccggctgagc aattgcgttg agcaatgcgt gcttggtcca ggtgtggaag
    21181 tgctcggtca gacgataggt cgtacccaca tacgggaact gctctttttt acccatccgc
    21241 agcgcgtctt gttcatacag acgaacaacc gggttagaca ccacgttcgg atgcagcggg
    21301 ttagtgccca gcggcgtttc aatcggctcg tagtgttccg ggaacggacc ttccgccatt
    21361 ttgttgatgg caaacaggcg tcccatccct tccggctgca tgataaacgg cccggttggc
    21421 gtacccggtg cggcattgcc gaagtccgga atatcgttac ccgtccactt gctgccgttc
    21481 cactggatca gcatccgttt cggatcccac ggtttaccat tgatatccgc cgaagcacgg
    21541 ttgtagagca cgcgacggtt gagcggccac gcccaggccc atcccagcgt attccccaga
    21601 ccggatgggt cggagttatc gcggttagcc atctggttgc cctgctctgt ccagctaccg
    21661 gtgtagatcc agcaagaaga tgcggttgta ccgtcatcac gcagatgcgc aaagctactc
    21721 agcaactgac ctttcttcgc aatcagcacg ccattagcgt catagagatc ttccagcgca
    21781 tagccgttat tctctttcgc cacttcgtca gattgcggtt cgtgcggctg cttgtagttc
    21841 cagctcatct tcatcagcgg ttctacgcct ttaccacctt cggcctggta cagctcgcgc
    21901 agatgatggt agatacccgc cagaatttcg ccgtcgttac gcgcttcgcc cggcgcgtcc
    21961 tgacctttcc agtgccactg caaccagcga ccggagttag cgatagaacc atcttcttcg
    22021 gcaaagcagg tcgaaggcag acggaatact tcagtctgaa tagacgccgg atcgacatcg
    22081 ttcgactcgc cgtggttctg ccagaaggta gaggtttcag tcaccagcgg atcgataacc
    22141 accatgtact tcagcttgct caggcagctc accactttgt ttttgtccgg gaaggacgca
    22201 accgggttaa agccctggca gaagtaaccg gtgactttgc cttcgtccat catgttgaaa
    22261 tacttgatga cgtcgtaggt ctggtcccac ttcggcagcc agtcatagcc ccagttgttc
    22321 tctttctgcg cggcatcgcc atagaaagat ttcatcaggc taacgaagaa cttcggatag
    22381 ttgctccagt agttcacctg atcagccagc gtcgctttcg gcgtgttcgc ttccagatac
    22441 gactgcaaat caacctgttt ttctgacgga agcgtcagat aacctggcag gctggtagag
    22501 agcagaccta agtcagtcag cccctgaatg ttggagtgac cacgcaatgc gttcacgccg
    22561 ccaccggcca tacccatgtt accgagcagc aactggatca tcgccatagt acggatgttc
    22621 tgcgcaccca cggtgtgctg cgtccagccc agcgcgtaca ggaaggtggt tgtgcgatcc
    22681 ggtgcgctgg tggaggccag cacttcacac actttcagga agtcggcttt tggcgtaccg
    22741 cagatgtttt caacgacgtc cggcgtgtag cgggaaacgt gcgctttcag caggttccac
    22801 acacagcgcg gatgagtcag tgtttcatcg cgttttgcat agccgttttc atcgaactga
    22861 tagttccagg acgacttatc atactggcgt ttttcagcgt cgtagccgct gaacaggccg
    22921 tcttcgaaag caaaatcatc acgcaccagc aggctggcgt tggtgtaatg tttaacgtat
    22981 tcggcgttga ttttgttgtt ttcgatcagg tagcgcaaaa cgccagacag gaacgtaatg
    23041 tccgtaccgg aacgaatagg tgcgtaaata tccgccacag aagcggtacg cgtaaaacga
    23101 ggatcgacaa cgatcaaggt tgcatcgttg ttgtttttcg cttccatcgc ccagcggaaa
    23161 ccgacgggat gcgcttcagc agcgttaccg cccattacca ttacgacgtt agcgtttttg
    23221 atatccaccc agtggttggt catcgcaccg cgaccaaatg ttggagcaag acttgctacc
    23281 gttggtccgt gtcagacgcg cgcctggttg tctaccgcca gcatcccgag ggagcgggca
    23341 aatttctggg tcagcatccc ggtttcgttg ctggcaccgg aggcacacag cataccggta
    23401 gaaagccaac ggtttaccgt tacgccctgc tcgttctttt caataaagtt agcgtcacgg
    23461 tcagctttca tcagcttcgc aatacgggag aatgcttctt cccagctaat gcgctgccat
    23521 ttgtcagaac ctggcgcacg atattccggg tagcgcagac ggttttcgct gttgacgtaa
    23581 tccagcaaac cggccccttt tgggcacagc gcaccacggc ttaccggatg atccgggtca
    23641 ccttcaatgt gataaatcgc ttctctggcg tttttcgcgc catcacccag gctatacatc
    23701 aatagcccgc aacctacgga acagtatgtg caggtgttac ggatctcttt agcgcgtaat
    23761 aatttgtagt ttcgcgcctg agccagtgct tgcttcgggg caaagcccaa tgccgctacc
    23821 gttgttccag ccataccgcc cgcgcagatt ttaaaaaatt gtctgcgact gacgtccatt
    23881 gctttcctct tttttcaggg gtattactgc gcgaggaaac taacagtaaa attctgaatc
    23941 tttttgcgcg aaatcaaaaa gaacacgtgt atgccacaaa ataatccgcc tgcgcaaatt
    24001 aacctacctc aatagcggta gaaaaacgca ccactgcctg acaggccagt taaaaaaatg
    24061 ctataaaatt cagcttaatt tttaacggca agagagacaa aacagcgagc atgacacgac
    24121 aaaaagcaac gctcataggg ctaatagcga tcgtcctgtg gagcacgatg gtaggattga
    24181 ttcgcggtgt cagcgagggg ctcggtccgg tcggcggcgc agctgctatc tattcattaa
    24241 gcgggctgct gttaatcttc acggttggat ttccgcgtat tcggcagatt ccgaaaggct
    24301 atttactcgc cgggagtctg ttattcgtca gctatgaaat ctgtctggcg ctttccttag
    24361 ggtatgcggc gacccgtcat caggcgattg aagtgggtat ggtgaactat ctgtggccca
    24421 gcctgacaat tctctttgcc attctgttta atggtcagaa aaccaactgg ctgattgtac
    24481 caggattatt attagccctg gtcggcgtct gctgggtgtt aggcggtgag aatgggttac
    24541 attatgatga aatcatcaat aatatcacca ccagcccatt gagttatttc ctggcgttta
    24601 ttggtgcgtt tatctgggca gcctattgca cagtaacgaa taaatacgca cgcggattta
    24661 acggaattac cgtttttgtc ctgctaacgg gagcatgtct gtggatttac tattttctca
    24721 cgccacaacc agaaatggta tttagcacgc ccgtcatgat taaactcatc tctgcggcat
    24781 ttaccttagg atttgcttat gctgcatgga atgtcggtat attgcatggc aatgtcacca
    24841 taatggcggt aggctcgtat tttacgcctg tactttcctc agcgcttgca gccgtgctgc
    24901 tcagcgcccc gctgtcgttc tcgttctggc aaggcgcgct aatggtctgc ggcggttccc
    24961 tgctctgctg gctggcgaca cgtcgtggtt aaaataattg ccgggtctac ggcccggcag
    25021 ttaatcagat aaattccgac aatattaaaa aataaatttc aaataccagt aattcacctt
    25081 atatctcatt tcgacttcga tcacatatcg ataaataata ttatttcatc tatttttctc
    25141 attgttttta atcaccattc tattttaaaa acagtatgtc atttataaat gacgccgttt
    25201 aaaattcgtt tagaaaatac gtttaacgat aattaaaagg attctgttaa atgaaattaa
    25261 aaatagttgc ggtggttgta actggtttgt tagctgcgaa cgtagcacac gctgccgaag
    25321 tctataacaa ggatggtaat aaactcgacc tttatggcaa ggttaccgct ctacgttatt
    25381 ttactgatga taagcgtgac gatggtgata aaacttatgc ccgtctcggc tttaaaggag
    25441 aaacgcaaat caatgatcaa atgattggtt ttggtcactg ggaatatgat tttaaaggct
    25501 ataacgatga agccaacggc tcgcgcggca acaagacccg tctggcctat gctggtttaa
    25561 aaattagtga atttggctct ctggactatg gtcgtaacta cggtgtcggc tacgacattg
    25621 gttcatggac cgatatgttg ccagaatttg gtggcgatac ctggagtcag aaagatgtct
    25681 tcatgacata ccgtaccacc ggtctagcaa cctatcgcaa ctacgatttc tttggcttaa
    25741 ttgaagggct gaactttgcc gcgcaatatc aaggcaaaaa tgaacgtact gacaacagtc
    25801 atctttatgg tgctgactac acgcgtgcca acggtgacgg tttcggtatc tcctcaactt
    25861 atgtttatga tggctttggt atcggtgcgg tatataccaa atccgatcgg acaaatgcgc
    25921 aggaaagagc cgctgctaat cctctcaatg cctccggtaa gaatgcagaa ctatgggcca
    25981 caggtataaa gtatgatgcc aacaacatct actttgcagc taattacgct gaaacattaa
    26041 acatgaccac ctatggggat ggttatatct ctaacaaagc acaaagtttt gaagtggtgg
    26101 cgcaatatca attcgacttc ggcttgcgcc catcactcgc ttacctgaaa tcgaaaggca
    26161 gagatctggg ccgctacggc gaccaggaca tgattgagta tatcgacgtt ggtgcgacgt
    26221 atttcttcaa caaaaatatg tcgacctatg ttgattataa aatcaacctg attgatgaaa
    26281 gcgactttac ccgtgccgta gatattcgga ccgataacat cgtcgctacg ggcattacct
    26341 atcagttcta acgtcctctc ctgaccagcc cttctctggg ctggtttttg atttaagtgc
    26401 tgctgtatga ctccttaagt aaaaatgagc aatttttgat atttctggtc attcacctca
    26461 attaatatta ttccatatcc tccggggaca tcttactata atatttattg atgagtgatt
    26521 atgaccaata ttaatacagc ttgtgtaaaa aataatgcca gttatcaact taataacgca
    26581 ttacctaaca aagaaaccat ctccagcaat ttttgtgaac gactagaaca atggggtaat
    26641 aaatcggtta ataatggtga agaaagagca attgccgtgg agcgaattaa agaagcttac
    26701 aattcgaaca tggcatctct tgatttgtca tatcttgatt tgagtgaatt accgcctatc
    26761 ccctcgacag ttaatacgtt aaatctggaa aacaattgtc taacttgtct tgacttagct
    26821 gacaatgcca gtctcgttaa tatcaacctc agcgttaata aaattaacac gataaccttt
    26881 ccaaaagaat caaaactgga aaacatttat attgatcata acaatctgga aagtttagat
    26941 ttaaaaaatc agcattcatt ggttaatctg gaagcgcaaa ataacaaact gacaaaaatt
    27001 aatatttctg atagttataa actgaaattt cttaatcttg attataacaa gctagcatcg
    27061 ctggatgtct cccggcaaga atctctgatt gagttaagtg cccatcacaa tatgatcact
    27121 gaccttatat tacacaatca tcccaggatg aaaacaatca ctttaaacga caaccatatt
    27181 gcacatttaa acgccaaaaa cactacaaaa ctggaatatt taaacttaag caataacaat
    27241 ttactgccaa ccaatgacat tgatcaacta atatcatcaa aacatctttg gcatgtatta
    27301 gttaacggca tcaacaatga tccacttgca caaatgcagt actggactgc agtaagaaat
    27361 ataattgatg acactaatga agtgaccatt gatttatcag gacttaattt aaccactcaa
    27421 ccaccagggc tgcaaaactt cacatctatc aatcttgata ataaccaact cacacatttt
    27481 gatgcaacca actacgatag actcgtaaag ctaagtctga atagtaatgc tcttgagtca
    27541 ataaattttc ctcaaggcag aaatgtaagt attacacata tatctatgaa taataatgct
    27601 ctcagaaata ttgatataga taggctttca tcagttactt attttagtgc ggcacata
//
LOCUS       DAESHI010000048        27470 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.048, whole genome shotgun sequence.
ACCESSION   DAESHI010000048 DAESHI010000000
VERSION     DAESHI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27470
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.048"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..81)
                     /locus_tag="KW011_002140"
     CDS             complement(<1..81)
                     /locus_tag="KW011_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 domain-containing protein"
                     /protein_id="HBH7660465.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTL"
     gene            complement(199..1638)
                     /gene="rsmF"
                     /locus_tag="KW011_002141"
     CDS             complement(199..1638)
                     /gene="rsmF"
                     /locus_tag="KW011_002141"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HBH7660466.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(1719..4352)
                     /gene="yebT"
                     /locus_tag="KW011_002142"
     CDS             complement(1719..4352)
                     /gene="yebT"
                     /locus_tag="KW011_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HBH7660467.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4321..5604)
                     /gene="yebS"
                     /locus_tag="KW011_002143"
     CDS             complement(4321..5604)
                     /gene="yebS"
                     /locus_tag="KW011_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HBH7660468.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5734..6231
                     /gene="msrC"
                     /locus_tag="KW011_002144"
     CDS             5734..6231
                     /gene="msrC"
                     /locus_tag="KW011_002144"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HBH7660469.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6328..7026
                     /gene="proQ"
                     /locus_tag="KW011_002145"
     CDS             6328..7026
                     /gene="proQ"
                     /locus_tag="KW011_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HBH7660470.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7046..9094
                     /gene="prc"
                     /locus_tag="KW011_002146"
     CDS             7046..9094
                     /gene="prc"
                     /locus_tag="KW011_002146"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HBH7660471.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9286..10167
                     /gene="htpX"
                     /locus_tag="KW011_002147"
     CDS             9286..10167
                     /gene="htpX"
                     /locus_tag="KW011_002147"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HBH7660472.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10213..11276)
                     /locus_tag="KW011_002148"
                     /pseudo
     CDS             complement(10213..11276)
                     /locus_tag="KW011_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11453..12244
                     /gene="kdgR"
                     /locus_tag="KW011_002149"
     CDS             11453..12244
                     /gene="kdgR"
                     /locus_tag="KW011_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HBH7660473.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12387..12626)
                     /locus_tag="KW011_002150"
     CDS             complement(12387..12626)
                     /locus_tag="KW011_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660474.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12785..12928
                     /gene="mgrB"
                     /locus_tag="KW011_002151"
     CDS             12785..12928
                     /gene="mgrB"
                     /locus_tag="KW011_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HBH7660475.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13003..13290
                     /locus_tag="KW011_002152"
     CDS             13003..13290
                     /locus_tag="KW011_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HBH7660476.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            13960..14103
                     /locus_tag="KW011_002153"
     CDS             13960..14103
                     /locus_tag="KW011_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HBH7660477.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14116..14325
                     /gene="cspE"
                     /locus_tag="KW011_002154"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14116..14325
                     /gene="cspE"
                     /locus_tag="KW011_002154"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBH7660478.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            14491..15300
                     /gene="rlmA"
                     /locus_tag="KW011_002155"
     CDS             14491..15300
                     /gene="rlmA"
                     /locus_tag="KW011_002155"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HBH7660479.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(15297..15863)
                     /gene="mntP"
                     /locus_tag="KW011_002156"
     CDS             complement(15297..15863)
                     /gene="mntP"
                     /locus_tag="KW011_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HBH7660480.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(16292..16750)
                     /locus_tag="KW011_002157"
     CDS             complement(16292..16750)
                     /locus_tag="KW011_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HBH7660481.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(16805..17656)
                     /gene="manZ"
                     /locus_tag="KW011_002158"
     CDS             complement(16805..17656)
                     /gene="manZ"
                     /locus_tag="KW011_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HBH7660482.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(17669..18469)
                     /gene="manY"
                     /locus_tag="KW011_002159"
     CDS             complement(17669..18469)
                     /gene="manY"
                     /locus_tag="KW011_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBH7660483.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLNNELD"
     gene            complement(18532..19503)
                     /gene="manX"
                     /locus_tag="KW011_002160"
     CDS             complement(18532..19503)
                     /gene="manX"
                     /locus_tag="KW011_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBH7660484.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            19966..21522
                     /gene="yoaE"
                     /locus_tag="KW011_002161"
     CDS             19966..21522
                     /gene="yoaE"
                     /locus_tag="KW011_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HBH7660485.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(21526..23124)
                     /locus_tag="KW011_002162"
     CDS             complement(21526..23124)
                     /locus_tag="KW011_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH7660486.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVQFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(23255..24619)
                     /gene="sdaA"
                     /locus_tag="KW011_002163"
     CDS             complement(23255..24619)
                     /gene="sdaA"
                     /locus_tag="KW011_002163"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBH7660487.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(24803..25381)
                     /locus_tag="KW011_002164"
     CDS             complement(24803..25381)
                     /locus_tag="KW011_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBH7660488.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(25385..26746)
                     /gene="pabB"
                     /locus_tag="KW011_002165"
     CDS             complement(25385..26746)
                     /gene="pabB"
                     /locus_tag="KW011_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HBH7660489.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            26820..26999
                     /locus_tag="KW011_002166"
     CDS             26820..26999
                     /locus_tag="KW011_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HBH7660490.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            complement(27119..27442)
                     /locus_tag="KW011_002167"
     CDS             complement(27119..27442)
                     /locus_tag="KW011_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HBH7660491.1"
                     /translation="MVNFKDKNMPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKY
                     LKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEE
                     LKALV"
BASE COUNT         7066 a         6771 c         6942 g         6691 t
ORIGIN      
        1 taacgttcga tcacccggcg attcaccgtg taattcgccg tcgtcgattt caaaagcgcc
       61 tatgcgcaca ctggttttca tcttcgtgct ctccatttag ctgccggttg tggtacgacc
      121 agtccgatcg caccattttt cagcctagtt gaggcagccg aatgtgccag tcaaaaagtg
      181 cgcttttttt gtgcgctgtc aggcgttacc ggtaaaaagt ttcccatcgc gcaccagttc
      241 acgcggatag ctgtttttca atcgcgaacc aatccgtttg gctaaaccaa tcggttgatg
      301 ctggaaagta accaacacgt catccgccac tggcgcggct tgcgggtaaa catcgcgccc
      361 gcgataccac tcctccgctt cctgcggtgt cagttcaaaa gcgttcacat tgtcgggggt
      421 ggcaagggca ataactgctt catgctgcca gcgataacct ttgttgtgcg tttcggcaag
      481 tttaatcccc aagcgagaaa atcggacttt accgatcagg gcttcaatgc ccaccgggaa
      541 caaccacagt tctttgtcac gctgccagag gcgcaggttt tcatcccagt ttaagccaac
      601 acctgcagcc gcctgacgaa tttgtccagc ttcgcgatct ttcactgggc tgaacggaaa
      661 attaccgact ttgtatttgg gggcgggtaa ggcgggaatc gcctgagttt tacgcagacg
      721 agcaacgaag aagccttcgc agtcgtaaat ttgtgggaaa acatgcaaaa agccttcctc
      781 ggtcagcgcc ttgtttgcac cagggaagag atcaccaagc ggtaaaaact ctactgcgtc
      841 ggggtaagtc tctttcagcc acaggcaaac ggcttcgttt tcttcctggt ttaaggtaca
      901 ggtcgagtaa accagcgtac cgccagggcg taatgcatga aaggcgctgt cgatcagttc
      961 ccgttgggta gctgcgattt cctgattgct ttctggtgac cagtttttta gcgcatcggg
     1021 atctttacgc accacgcctt cgccggagca gggagcgtcc agcaaaatgg catcaaacat
     1081 ttctggcact gccgcaccaa acacgcggcc atcaaaatgt gtgagcgcaa cattactgat
     1141 accacagcgg ctgatattgg catgtaacac ttttacccga ctggcggaaa actcattggc
     1201 aagaattgcc ccttcgttat tcatccgcgc ggaaatttgc gtcgttttgg agccaggcgc
     1261 ggcagcgaca tccatcaccc gctgtggtgc attaccgtca gcaaacaagg cggcgacggg
     1321 caacattgaa ctggcttcct gaatataaaa caggccactt aaatgctcgg cggtactacc
     1381 caatggcaat gcatcttcat tgtcgcgttc aatccagaaa ccttcttcac accacggaat
     1441 tggcgtaagc gtccagccat aaggagcggt taattgcagg aaatcagcaa cggagatttt
     1501 cagcgtatta acgcgaatgc tgcggcgcaa cgggcgctga caggcggcaa gaaaatcatc
     1561 aaaagagagc gtcgaaggca tcgcttcgcg catttgcgtc agaaaggcgt ccgggaaata
     1621 aacggtgtgt tgggccacga gcatatacca ctggcaaaaa aaacaggcgc gcagtttagc
     1681 ataaacgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagtaccc
     1741 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgcttgcc ttcctgggct
     1801 ttcggtgcca gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaactgg
     1861 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     1921 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     1981 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2041 agaccacgga acagcacagg cgtaccaata cctaacgaac cggcttccgg tgcttcgaca
     2101 ataatgctta agccatccag gtaacgcgaa tcagtaatag tagcttcttg caactcaaag
     2161 tcacggcgag gattaccccg acctggttcg acattgatat acggctggag gatagtatct
     2221 agatgctcaa cgcccgcggc tgaaatttgc ggtgtgacca ccgagaagcg cgtaccgccg
     2281 cgggcgaagg tctggacata ctccggatag agcaccgcct ttgcctgtac ttcattgcgc
     2341 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     2401 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgccc accaaccgca
     2461 cgggccgctg tttcggaagc atacagaatt cgtttgtcgc ctttacgctg actggcgctg
     2521 gcaccgctga ggttatcgaa gctaatggct ccctttaatg ctctggagag cggggatgcc
     2581 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     2641 ctattgctgg tcagaaggtt gcgatactcc ggcttaatat gcagatcgat atcaaacgcg
     2701 ttagctcgcg gacgcacggt aatcacttca ccaacttcaa atttacggta cagcactacc
     2761 gagcctgcct gcacatccgg cagcgtctct gcactcaaac tcacggttgt ggtgggtaaa
     2821 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     2881 atctcacctt tatcgcctgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     2941 gcaccaagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3001 aatttgctat cgccttttac cagttcgcga tgctgaggct cgatggcgac ggtaaaggta
     3061 acgcctttgc tggtgagttt acgatcaata acctggccta cctgcacgcc gtgaagaatg
     3121 agcggctgac cagcatcaat accgtaactt tccggcgcgg tcagggtcag cgtcagaaca
     3181 tcaggttcat gcagcagtgc tttttcgcct ggcacaacaa cgaactcttt gcgtggctcg
     3241 ccatcgccgg gtaccaactc gaaggttttg ccagtcagca gggcgctgag attggcatcg
     3301 ctaagggata atttcggatt aagtaattcg atgcgggtat tttcacgaag cagggtaacg
     3361 acgctgggat taactgtcat ttccccagtg actttaccac caggatttaa atccagttta
     3421 gttagctgtc cgacttccag tccctgatac attaatggcg tcgagtcggc ggttaatccg
     3481 gccccactcg gcagttccag tttgattatt acgccacgct ggctatgggc gaggtcttca
     3541 tacagaccaa aggtatcttc cgcctcggca ggtttcgact cttcaggtga atcgaaggca
     3601 atcgcaccgt taaccagtgc cgccagactt tccagtttca ccttcgcgcc actgatactg
     3661 acgttggcat caacgccgga aacgttccag aaacggctac cttttttcac caggtcggta
     3721 aaacgccgct cgatcaggac atcaatcacc acgccttgct tgttgggatt gatggcatag
     3781 tcgtagactt ttcccacagg gatcttgcgg aaatagacca atgaaccgct gttcagcgaa
     3841 ccgagatcgg gggcttgcag gtggatcatc agatcgccat tgtccagtcg atatttcggt
     3901 tgggtatcga gtgcgacaaa gtgatcctgc tctttacctt tacctggcat catgccgata
     3961 tagttaccac cgacgagggc gtccagcccg gagacacctg ccaacgatgc ttttggcgtc
     4021 accagccaga actgagtctc ttcgcgcagc gcatctttca tatcggactt gatgctgacc
     4081 ttgacttcaa tcttacgaag atcgtcgctg aggctgatat cctgcactgt tccgacttca
     4141 acgccctgat aacgaacagg cgtacggccc gggacaatac catccgccga cataaagtcg
     4201 atggtgacgg tattaccccg gtcctgataa ctgtcccaaa tcagccaacc ggcaatcatt
     4261 agcgcgatga aaggcagcag ccagaaaggt gagatacggc gtttattttt aatctgcgct
     4321 tcagtcgtcg aagcgggcgt ttcctgactc atgtgcatcc caaagtaagc ggctgtccag
     4381 ccattccaca gcaagaatag tcaaaattac ggctgcgcca aaataaaacg cagccggtcc
     4441 catagtaaaa gcgaggatct gatcgcgatt aatcagcgac atggttaaag atatgacaaa
     4501 caggtcgagc atcgaccagc gtccaatcca ggtcaccatc cgcagtaaca gaatgcgtgt
     4561 gcgtaaacct tgctggcatt taaaatgaat gctcaacagt aaagtgaaca tgacgatcac
     4621 tttagtaaac ggtaccagaa tactggcgat aaacacgatt ccggcgaccg caatgttgct
     4681 acttgccagc gacataatcc cggaaagaat cgtatcttcc tgccggccac cattcagata
     4741 aatgatagaa ataggcaata ggttggctgg taacaacaaa acgattgacg ctaacagcgc
     4801 cgcccagcat ttttgcaaac tatgacgacg acgcaggcgt agcgggatat ggcaacgcgg
     4861 gcagcgacca cgctgatcag gatagccggt aaaatggcac ccaagacaga cacgaagttt
     4921 ctcgtcccta cgcgtagcgg ggcgctgcgg ataatatcgc tcccacagct cctcaacatt
     4981 aagatgtgac aacgtcaccg tcgttaaaat caccaacgcc acaaaagaga acaagccgac
     5041 acccgcctgg atatgggcat aatcctgtac ctttatagaa gcaacgccaa tgccgaccag
     5101 gtagatatcc agcattaccc actctttaag acgctcaagc atcagcagta ccggacgtaa
     5161 attcattccc agtcggttac caaaccataa atatgctatg gaggacacca gaatgagggg
     5221 ggcaccaata acgcagaaaa agaccatcgc ccccgttatc gtatcgccct gtttggtcat
     5281 ttgccagatg ccttgcatga cgttggcgtc gatacgaata cctaacagcc agatatgcaa
     5341 cagcggttcg ccccaggcaa acggcatcag caacagcatg gtgaaagcca ttgccgccag
     5401 gcgcgttagc gaccagtcgc gcccgtcgcg aatttttgcc tgacagcgcg gacaataggc
     5461 actctgatga gaatttatct cgggcaggct aaacagcatg tcacattgcg ggcaacgttg
     5521 gtaatcacca cgcggcagtt cctcgccgat tgccctcact gttatctttt ttgtcggcgt
     5581 aatttgtggt gtgttaagag ccatgacgtg ccaaatgtaa taagaattaa cattaatctt
     5641 aacccatgat gggaatgatc ttgagcatta acgcatttaa tgcttatttt aataggctgg
     5701 cagttccagg taagacaaac aagtgattat ataatgaaca aaacagaatt ttacgcggat
     5761 ttaaatcgcg actttaacgc gctgatggcg ggagaaacca gttttctggc aacgcttgcg
     5821 aacaccagtg cgttgttata cgagcgtctc actgacgtaa actgggcagg tttttatttg
     5881 cttgaggacg atacactggt actcggacca tttcagggca aaattgcctg tgtccggata
     5941 cccgtcgggc gcggggtgtg cggcactgcg gttgcccgca atcaagtgca gcgtatcgag
     6001 gatgttcacg cgtttgacgg gcatattgcc tgtgatgcgg cgagtaattc tgaaattgtt
     6061 ctgccgctgg tggtgaaaaa tcagattatt ggtgttcttg acatcgatag taccgtcttc
     6121 ggtcgcttta cagacgagga cgagcaaggc ttacgtcagc ttgtggcaca gcttgaaaaa
     6181 gtgcttgcaa cgacggatta caaaaaattc tttgcgagcg tcgcaggata atcaacggat
     6241 aacgtagcat ttactggtag cgtcattata atgacgcctg ttcatgcctg cgcttgttgg
     6301 ctacgtccgt tgtaatcagg aaatttcatg gaaaatcaac ctaagttgaa tagcagtaaa
     6361 gaagtaatcg cgtttctggc cgaacgtttt ccccactgtt tcagtgcgga aggtgaagcg
     6421 cgtccgctga aaatcggtat ttttcaggat ttggtcgatc gtgttgccgg ggaaatgaac
     6481 ctgagcaaaa cgcaattacg atccgcttta cgtctctaca cttcgagctg gcgttatctt
     6541 tacggtgtta aacccggcgc aacgcgtgtc gatcttgacg gcaacccatg cggtgagctg
     6601 gacgagcaac atgtagagca tgctcgcaag cagcttgaag aagcgaaagc gcgtgttcag
     6661 gcacagcgtg ctgaacagca agcgaaaaaa cgcgaagctg ccgcagctgc tggtgagaaa
     6721 gaagacgcac cgcgccgcga acgcaagcca cgtccgacca cgccacgccg caaagaaggc
     6781 gctgaacgta aacctcgtgc gcaaaagccg gtagagaaag cgccaaaaac agtaaaagca
     6841 cctcgcgaag aacagcacac cccggtttct gacatttcag ctctgactgt cggacaagcc
     6901 ctgaaggtga aagcgggtca aaacgcgatg gatgccaccg tattagaaat caccaaagat
     6961 ggcgtccgcg tccagctgaa ttcgggtatg tctttgattg tgcgcgcaga acacctggtg
     7021 ttctgaaacg gaggccgggc caggcatgaa catgtttttt aggcttaccg cgttagctgg
     7081 cctgcttgca atagcaggcc agaccttcgc tgtagaagat atcacgcgtg ctgatcaaat
     7141 tccggtatta aaggaagaga cgcagcatgc gacggtaagt gagcgcgtaa cgtcgcgctt
     7201 cacccgttct cattatcgcc agttcgacct cgatcaggca ttttcggcca aaatctttga
     7261 ccgctacctg aatctgctcg attacagcca caacgtgctg ctggcaagcg atgttgaaca
     7321 gttcgcgaaa aagaaaaccg agttaggcga tgaactgcgt tcaggcaaac tcgacgtttt
     7381 ctacgatctc tacaatctgg cgcaaaagcg ccgttttgag cgttaccagt acgctttgtc
     7441 ggtactggaa aagccgatgg atttcaccgg caacgacact tataaccttg accgcagcaa
     7501 agcgccctgg ccgaaaaacg aggctgagtt gaacgcgctg tgggacagta aagtcaaatt
     7561 cgacgagtta agcctgaagc tggcaggaaa aacggataaa gaaattcgtg aaaccctgac
     7621 acgccgctac aaatttgcca ttcgtcgtct ggcgcaaacc aacagcgaag atgttttctc
     7681 gctggcaatg acggcgtttg cgcgtgaaat cgacccgcat accaactatc tttccccgcg
     7741 taataccgaa cagttcaaca ctgaaatgag tttgtcgctg gaaggtattg gcgcagtgct
     7801 gcaaatggat gatgactaca ccgttatcaa ttcgatggtg gcaggtggtc cggcagcgaa
     7861 gagtaaagct atcagcgttg gtgacaaaat tgtcggtgtt ggtcaaacag gcaagccgat
     7921 ggttgacgtg attggctggc gtcttgatga tgtggttgcc ttaattaaag ggccgaaggg
     7981 cagtaaagtt cgtctggaaa ttttacctgc tggtaaaggg accaagaccc gcactgtaac
     8041 attgacccgt gaacgtattc gtctcgaaga ccgcgcggtt aaaatgtcgg tgaagaccgt
     8101 cggtaaagag aaagtcggcg tgctggatat tccgggcttc tatgtgggtt tgacagacga
     8161 tgtcaaagtt caactgcaga aactggaaaa acagaatgtc agcagtgtga tcatcgacct
     8221 gcgtagcaat ggcggtgggg cgttgaccga agcggtatcg ctctcaggtc tgtttattcc
     8281 tgcgggtccc attgttcagg tccgcgataa caacggcaag gttcgtgaag acagcgatac
     8341 cgacgggcag gtgttctata aaggcccgct ggtggtactg gttgaccgtt tcagtgcttc
     8401 ggcttcagaa atctttgccg cggcaatgca ggattacggt cgtgcgctgg ttgtgggtga
     8461 accgacgttt ggtaaaggca ccgttcagca ataccgttca ttgaaccgta tttacgatca
     8521 gatgttacgt cctgaatggc cagcgctggg ttctgtgcag tacacgatcc agaaattcta
     8581 tcgcgttaac ggcggcagta cgcaacgtaa aggcgtaacg ccagacatca tcatgccgac
     8641 gggtaatgaa gaaacggaaa cgggtgagaa attcgaagat aacgcgctgc cgtgggatag
     8701 cattgatgcc gcgacttatg tgaaatcagg cgatttaacg gcctttgggc cggagctgct
     8761 gaaggaacat aatgcgcgta tcgcgaaaga tcctgagttc cagaacatca tgaaggatat
     8821 cgcgcgcttc aacgctatga gggacaagcg caatatcgtt tctctgaact acgctgtgcg
     8881 tgagaaagag aataatgaag atgatgcgac gcgtctggcg cgtttgaacg aacgctttaa
     8941 acgcgaaggt aaaccggagt tgaagaagct ggatgatcta ccgaaagatt accaggagcc
     9001 ggatccttat ctggatgaga cggtgaatat cgcactcgat ctggcgaagc ttgaaaaagc
     9061 cagacccgcg gaacaacccg ctcccgtcaa gtaatatcaa tcaggcacaa gaaattgtgc
     9121 ctgatttttt aacagcggca agatgccgta aatcagatgc tacaaaatgt aaagttgtgt
     9181 ctttctggtg acttacgcac tatccagact tgaaaatagt cgcgtaaccc atacgatgtg
     9241 ggtatcgcat attgcgtttt gttaaactga ggtaaaaaga aaattatgat gcgaatcgcg
     9301 ctcttcctgc taacgaacct ggccgtaatg gtcgttttcg ggctggtact gagcctgaca
     9361 gggatacagt cgagcagcgt tcaggggctg atgatcatgg ccttgctgtt cggttttggt
     9421 ggttccttcg tttcgcttct gatgtccaaa tggatggcat tacgatctgt tggcggggaa
     9481 gtgatcgagc aaccgcgtaa cgaaagggaa cgttggctgg tcaatactgt agcaacccag
     9541 gctcgtcagg cagggatcgc tatgccgcaa gtggctatct accatgcgcc ggacatcaac
     9601 gcttttgcaa ccggtgcgcg ccgtgatgcc tctctggttg ctgtcagcac cggtttgctg
     9661 cagaacatga gcccggatga agccgaggcg gtaattgctc acgaaatcag ccacatcgcc
     9721 aatggtgata tggtcaccat gacgctgatt cagggcgtgg tgaacacctt cgttatcttt
     9781 atttcccgta ttctggcgca gcttgccgcg ggttttatgg gcggaaatcg tgatgaaggt
     9841 gaagagagca acggcaaccc gctgatctac tttgcggttg caacggttct ggaactggtg
     9901 tttggtattc tggcgagcat tatcaccatg tggttctcgc gtcatcgtga attccatgct
     9961 gatgccggtt cggcaaaact ggttggtcgc gagaaaatga ttgccgcgct gcagcgcctg
    10021 aaaaccagct atgaaccgca agaagcaacc agcatgatgg ctttctgcat taacggtaag
    10081 tcgaaatcgc tcagtgagtt gttcatgact cacccgccgc tggataaacg aattgaagct
    10141 ctgcgtacgg gtgaatacct gaagtaatat gaagtaaata ctaaaaagcg cgtcctgatc
    10201 gacgcgcttt ttttatgccc tggatcgtgg ctgagtgata cgtaaaccac tgacacaggc
    10261 agcaatcact gccagaatag ccgcagccat cagcgaaacg tgcgtaccat tatcaccgaa
    10321 ctgatttagc atcagcgcca ccagtgccgc gccgctactc tgacccagta gacgagccgt
    10381 tcctaacatg ccactggctc caccgctacg ttcgcgagga gcggaggtaa taatggtgtg
    10441 gttattgggt gactggaaca agccaaatcc agcgccacat aagatcatcg gccagataat
    10501 attgatatcc gcaggtgacg cgggcagcag aaccagggga aaaagccccg cagccatgat
    10561 gaacaaccct agagcaccca gcaatcctgc atgtacgcgt tcaatcaaat agcctgccaa
    10621 cggagccatc accatcgttg ctaacggcca cggtgtcaga agtaaacctg tttcgacttc
    10681 actacgcccg agcacggttt gcaggtaaaa gggcagggaa accattgcca gcatttgtgc
    10741 gcagaaagag caaacagatg tgcaaataga aagtgaaaac agcgggatac gcagtaaatc
    10801 caccggtagc agcggtacgg gaagagaaag ctggcggcga ataaagaaaa taccaacaac
    10861 aaccattacc accagttccg cagcaattaa cgtcagcgat tgcccctgag cgaaaccact
    10921 caacgcagtg ataagcaggc cgaaggttaa cgcgttcatc acggcgctgg gcaggtcgaa
    10981 acggggttta ctggcgcgag aaccattggg tggcagaaaa cgcatcgcca gaagcagggc
    11041 gataatacct aacggtacgt tgattaaaaa taaccatttc caggatgcga tggagaggat
    11101 tgctgcagca attgtcggcc cggcagcaga agagacggca acaatatggc gattgttggc
    11161 agggcgacgt tggcgattgc gccgtcaagg acagccatcg aaatgccaat cacaatggtt
    11221 aatatcgcac cgtatcgctg gggcaatggc aggccgtcgg cctgaacttt tggcatagga
    11281 attttatatc tttggtgaat aatcaacagg ggtatgattc tagcactgat attcacggtg
    11341 gatgtcgcag aattgtaact aaccggcaaa agatggattg cgaccaccct ggtgcgaaat
    11401 tataataaaa aacagttctg atttttataa aacactcgca atgaggtgat aaatggctaa
    11461 cgcagatctg gataaacagc ctgattctgt atcttccgtg ctaaaagttt ttggcatttt
    11521 gcaggcgctg ggtgaagagc gcgaaatagg gataaccgag ctgtcgcagc gcgtcatgat
    11581 gtcaaaaagc accgtttatc gctttttaca gaccatgaaa accttaggtt atgtggcgca
    11641 ggaaggggag tcggagaaat attccctgac cctgaaattg tttgaactgg gcgctcgcgc
    11701 gttacaaaac gtcgatttaa ttcgtagcgc agatatccag atgcgtgaga tctcccgcct
    11761 gaccaaagaa actatccacc tcggcgcact ggacgaagac agtattgttt acattcacaa
    11821 aattgactct atgtacaatt tgcgcatgta ttcacggatt gggcgtcgta atccgctgta
    11881 cagcaccgcg attggtaagg tactgctggc atggcgcgat ctcgatgaag tgaagcaaat
    11941 tcttgagggc gtggagtata aacgcagtac cgagcggacc atcaccagta cagaagcgtt
    12001 attacccgtt ctggaccagg tgcgcgagca ggggtatggc gaagataacg aagagcagga
    12061 agaagggctg cgatgcattg cggtaccggt atttgatcgc tttggcgtgg tcattgccgg
    12121 tttgagcatc tccttcccga cgttgcgttt ctctgaagag cgtttacagg aatatgtcgc
    12181 aatgttgcat accgcagcgc gcaaaatttc tgcccaaatg ggttatcacg actatccgtt
    12241 ctgatgagag taagaacctg tcggaatatc aaacagacag gttctttatt tagcatgaga
    12301 aaaataaagt tgaaggtgcg ttatattaaa cgcgcttgct ataagaatat tttactcatg
    12361 agtgagaatc tggttgttta ttacccttaa ccattatcga ccacgatatt gcttttacgt
    12421 aacagtgggc aatctgttat ccccaagaaa ccacttttag tgtgcaagta ttgtaccgtg
    12481 ctggtgcctc tggcagtcag ataggtacat tgcaaaccta atcctgcggc attctctttg
    12541 cttccaatca aaacgccata tccgctgagt aataatccta tccataccag tgctatcagc
    12601 ataactgtgc gaatgatgaa tcgcattaca acctcttctc tttttatgtt cgcttaatcg
    12661 tagcggcaat atgcgctgaa gcaagctact cattccgaaa aagcacgaat atcgacatag
    12721 ttaggcgctg tttaactaac gcatgctagt ttaatgacat aaggtaggtg aagcggagat
    12781 tggagtgaaa aagtttcgat gggtcgttct ggttgtcgtg gtgttggctt gcttgctgct
    12841 ttgggcgcag gtattcaaca tgatgtgcga tcaggatgta caatttttca gcggaatttg
    12901 tgccattaac cagtttatcc cgtggtgata tcattttttc agcactagat tttcttcctc
    12961 tccagcagtg gtagaatagc gcctctactc agaggtggtg aaatgaacga agttgtaaat
    13021 tcaggcgtga tgaacattgc gtctttggtt gtatcggtgg tggttcttct tatcgggctc
    13081 atcttgtggt tttttatcaa tcgtgccagt tcacggacta acgaacagat tgaactgctt
    13141 gaggcgttgc tggatcagca aaaacgtcaa aatgcactgt tacgtcgttt gtgcgaagca
    13201 aacgaaccag agaaagcaga taaaaagacc attgagagtc aaaaatcggt tgatgacgaa
    13261 gatattattc gcctggtcgc cgaacgataa tccttcctca atcttgagta accccgtcgt
    13321 tgaagggtta ctcaattcat cctgattata cgcactgtca tggtgcaact gcttcaaatt
    13381 aaaactctgg tctgacttca aataattatc atattattta tttgcgtaac gggatatcag
    13441 aaaaagatat tttcctcatt aatttattag gatgtttaca tcggatttgt gattaagcgt
    13501 ggtattattt attagcgaaa cgtttctctc ttgatttttt tgctcattca tcaatttttc
    13561 ttattttaaa ttttcaatcc ctttggggat tgacttctct ttagggtaat tgatagccgt
    13621 taaccgactg ttttatgaga aaaagtgata taacttttta ttcattgcat agcaaaaaat
    13681 gtgatattgc acgcactatg taataacttc tcccactggc ctggaacaac tgaacttatt
    13741 gaactatgtt agaaaatacg ccagtttaag tatctgcctg aactggcaag gttaagcaca
    13801 atgatatatc ggcgcgtatt ccgttgcata agtgtgcaaa aaaagtggaa gacgtatcga
    13861 gatttgtgcg tctgatcgag acatgtttaa aaatggcttg ccataattaa cgttgtatgt
    13921 gataacagat ttcgggttaa acgaggtaca gttctgttta tgtgtggcat tttcagtaaa
    13981 gaagtcctga gtaaacacgt tgacgttgaa taccgcttct ctgccgagcc ttatattggt
    14041 gcctcatgca gtaatgtgtc agttttatct atgttatgcc tgcgggcgaa gaaaacaatc
    14101 taaggaattt ttcaaatggc aaagattaaa ggtcaggtta agtggttcaa cgagtctaaa
    14161 ggttttggct tcattactcc ggctgatggc agcaaagacg tgttcgtaca cttctccgct
    14221 atccagggta atggcttcaa aactctggct gaaggtcaga acgttgagtt cgaaattcag
    14281 gacggccaga aaggtccggc agctgttaac gtaacagcta tctgatcgaa tccactgatc
    14341 tgaagtgtga atacgcttca atctcgctat aaagcctcgt cgaatgcgag gctttttact
    14401 atgctttatc ttcgctcctg gcgttcggat atttgcccgc cgcgtgattc gcgttacact
    14461 tgcggccttt agtatcctgc cggagttgtc atgtcttttt cctgtccact ttgccatcag
    14521 cctctttcgc gtgaaaaaaa cagctatatc tgtccccagc gacatcagtt tgatatggcg
    14581 aaagaagggt atgtcaatct gctgtccgtt cagcataaac ggtctcgtga tccgggcgac
    14641 agcgcggaaa tgatgcaagc acgccgcgca ttcttagatg ccggacatta tcagccgctg
    14701 cgtgatgcaa ttgtcgccca actgagggaa cggcttgatg ataaggccac ggcggtgctg
    14761 gatattggct gtggtgaagg gtattacaca cacgcatttg ccgatgcgtt gcccgaaatc
    14821 accacgtttg gtctggatgt ttcgaaggta gcgataaaag cggcggcgaa acgctatccg
    14881 caggtcactt tttgtgtcgc ttccagccac cgtttgccgt tttccgatac cagtatggac
    14941 gccataatac gtatttacgc gccgtgtaaa gcagaagaat tagcacgagt agtgaagccc
    15001 ggcggctggg tcattactgc cacgccggga ccgcgacatt tgatggagct gaaggggctg
    15061 atttacaatg aagtacatct tcatgcacct catgcagaac aactggaagg ttttacatta
    15121 cagcagagtg cggagttgtg ttatccgatg cgtcttcgcg gtgatgaagc cgtcgcatta
    15181 ttgcagatga cgccgtttac ctggcgtgcg aagccagaag tctggcaaac actggcagca
    15241 aaagaagtgt tcgactgcca gacggacttt aatattcacc tctggcagcg ttcttattaa
    15301 ccgtggaagt gcgtccagag gatctggacg ccgatgccga tcagtaccag cccgccgaga
    15361 atttccgctt ttttcccaat aattgagccg ataaagcgac caaccatcat ccctaatgtt
    15421 gacataatca aggttgcaca accaatggcc aatgcggtcg cgataatgtt gacctgcagg
    15481 aaagcaagac caacacccac agccatggca tccaggctgg tggcaatcgc ggtggttacc
    15541 agtagccaga aaccgtgtcg acggcgcggc tcttcatctt catcatctgc gccacgaaaa
    15601 ccctcaataa tcattcgccc gccgaggaat atcagcagca caaacgcaat ccagtggttc
    15661 cattcaagga caaaccggct ggctaacatg cccattcccc agccgatcag cggcgtcagg
    15721 gtttcgacgg caccaaaaat aaggccggtt cgcaatgctt cagaaaattt cggtttatgg
    15781 agggtggcac ctttaccgat tgatgcagca aatgcatcca tcgacatacc aaacgcaaga
    15841 agaacagtag cagtgatatt catgacaatg tcctgaccgg ggtatccata aaacacatca
    15901 tcgcccccag caaacatgca tacatgaaca acaagccgtg atggttttcg cgttcatgca
    15961 ggcatagcga ttgatgtgca tatggtctcg cctgactgaa agtattcagt ccgtacgtgc
    16021 cacgttttgc aacgagtatg ttgacacgta catttccgga atctggaaat cggctactcc
    16081 ccaaatgagg gcgcaacctt aacatatttt gagaatataa aacaactgtt ggaatatatt
    16141 atttcattgc tttattgata acgattttca tttggattta tatatcaatg aaacgttaaa
    16201 ataaataaca aataagggaa gaggtaatat agcctaagct atgtctgatt attaaaaata
    16261 cactaaacat agctttggct aaattcataa cttattgagt tgaaacgaga agcttataaa
    16321 ttttttccag atcgtcgata tttcgaacgc ggattaacag ccgacgctgt tctaattgca
    16381 tcaccaacac gccatcttcc gacaagttca tcgctttgat tcggctatat tcaatccaga
    16441 cattggcgaa gaaaaaacct ttttgtttaa agatgatctt cggaacgcgg atccagaata
    16501 tataaaaacc catcagagcc agtgcgctta ataaccatgt cgttattaac gcaccatgat
    16561 tcgtgacgtt gttatagata agaatgacaa tcaatccgac gaagataacg ctatcgatgc
    16621 gaccacgccg gagcaaagga attgccagca gggtggggcc gttacggcgg ggcatgatga
    16681 actgatcgta gatcgcgaag gccaggagtg cggcgatgaa aagaatcagt accaggtccg
    16741 tgattgtcat taatcctcca gataaaaaaa cggggccaaa aggccccggt agtgtacaac
    16801 agtcttacag tcccagcagg ccgcaagcgt aaccagcgat accgatgacg aagaagccaa
    16861 cgatgatcca cagcgggtta acttttttgc gcagtagcca catacaagca aaggtcagca
    16921 gcagtggtac caggcctggc attaactggt ccagaatagt ctggacagta gtaacgtgtt
    16981 ctttgcccgt ctggtcagta atgcgagaga caaccagcgg gatgttgaca tgtgtccact
    17041 tgttaaccaa tgcccccatg acaaacaggc cgaggataga cgccccttcc gtcagttttt
    17101 gcaggaagcc accacccata tctttaacga tatcgatacc tttggagtaa ccatacgcta
    17161 cgccgtagta acgggttgcc agacgcacca ggttaaacag gatgaagaac agcagcggac
    17221 ctaacaggct gccgctcatc gcgataccgg cacccagtgc tgcaaatacc ggacgtacgg
    17281 ttccccagaa gatcgggtcg cctacaccag ccagcggccc catcaaaccg actttgatac
    17341 cgttgatagc accgtcgtcg atctctgcgc cattagcacg ctgttcttcc agcgccaggg
    17401 ttacgccgag aatcggcgca gccacgaacg gctgggtgtt aaagaactcc aggtgacggc
    17461 gaatagcttg tttacgagct tcgttgttct cagggtagag gcgacgaatt gccggtacca
    17521 tagagaagca gaaacccagt gcctgcatac gttcgaagtt ccatgaaccc tggaagaggt
    17581 tagaacgcag gaagacgcca cgaatatcac tttgagtgag ttttttctcg gtggtagttt
    17641 gagttgtatc aaccatttcg ctcacctgtt agtccagttc gttattgaga tcgttgttac
    17701 cagctgcctg agcaggcgca ccggctacgc ggttgtattt cgggctaagt tggatgtaga
    17761 gcactgccat aacagtacca atcacaccca gagcaaccag gttaaagttg gtgaatgctg
    17821 cggttacgaa gccgaggtag aagaacggca tcaggtagcc agcacgcatc atgttgataa
    17881 ccatcgcata accaaccacc acgatcatgc caccggcgat attcagacca ttggtcacca
    17941 cttccggaat cgcattcagc atgttctgta cttcgctggt accaacagac agcgcaacga
    18001 tgacggccgg aatagccaca cgcattgctt gcaggaacag agaagaaacg tggatccagg
    18061 aaatcgctgt caggttgccg ttatcagcag ccttatccgc agcgtgctgg aaagcaacgg
    18121 taatagtacg aacgatgatg gtcagtacct ggcccgcagc ggccagaggg attgccagtg
    18181 cgatacctgc accaatgctc tgatgacctg cgataaccag aatggtagaa atgatagaag
    18241 ccagagcggc gtcaggcgca actgcagcac cgatgttcat ccagcccagc gcgatcattt
    18301 ccagcgtacc accgataata ataccggttt tcatatcccc aagaacgata cccaccaggg
    18361 tacacgcgat tagcggacgg tgaaactgaa attcatcgag gattgatccc atacctgcga
    18421 tacaggctac gataaatacc agcacaattt gaagagtggt aatctccatt gtacttctcc
    18481 tgtttacgta agacttaagt gtgaaaactg agtgataatc aacacaatac gttacttatc
    18541 gattttgctg atcagatcca tcattttcag tttcggatcg gtggaaacct tacggacttc
    18601 cagctcaata ccgcgcgcat tcagtttctt gaacgcctca atatcttttt catcaaccga
    18661 aaccgcgtta ttcacctggg ttttaccctg acggaatgcc ataccaccga cgttaacaga
    18721 ggtgattttc acgccgcctt caacgagacg ctctacatct gttgggttgg taaataacag
    18781 cattacgcgt tcgccagcat atttcgggtt gttgtagacg cgaatcattt tggcaacatc
    18841 aactacgtgt gctgttacgc ccggaggtgc aacctgggtg agcagtgtct tacgaacggt
    18901 atccgcagcc acttcatcac taacaacaat aatacgggag acattggttt ctttggtcca
    18961 gcgggtggcg acctgaccgt gaatcagacg gtcgtcgata cgcgcaaggc caataaccat
    19021 gtagtcgttt ggccccattg gttttgccgg agttggagcc gcttttggtg ctgcggcagc
    19081 gggtgctggc gcggcttttt caaccggttt ggctttcagt gctttcacgc cttcacggcc
    19141 tgtttctact gccagcgcaa ccagttcatc aaagcttggg tcatcatcac gagccattaa
    19201 cgtttccacg agcattggaa tgttaacgcc tgcaatgact tcataatgct ctttgtcgac
    19261 gacaatgcgg ctggcagcat tgaacgggct gcctccccat gtatcaacga gaaacagcac
    19321 gcctttagtg gtgtcgagtt ttgccaactg agcgttgtac ttttcaatca gcgtttcggc
    19381 attttcacct ggaacgaaat cgatccagcc gacgttttcc tgctcgccta acagcatttc
    19441 tgccgtttta agcaactgct ctgcagccca accatgtgtg cctataacaa tagcaatggt
    19501 cacttgctac ctcctttatt atcgttaaca cctcaacgtg ccagatgtat ttttgaatcg
    19561 tcgtccacaa tcgaatcgat tcagataagg gctacaacca aaaacccctg cgtttcgcga
    19621 tttattttag atatcgaaaa aattatttta tgtgatgaag atccgtaatt taactttcga
    19681 ttagcagaaa tttcgaaagg taaaatatcc ttgtcacatt cgtttgcaaa ggaaggtaaa
    19741 tctttgccaa atcagaggcg tctctgatat gtttaactcc cgtttaacaa ccatggagta
    19801 tggggcagta gcccgcagta tggatcgtca ccgacgtcat ttcagcatca ggccttttaa
    19861 cgcctgcctt tctggcactc tatgccgcac ctttcgtttg cattttgtcg ttacgcctgc
    19921 attatttctg gcgtcgaata gctattcctt aagcaggagc ttgtcatgga attcttaatg
    19981 gacccctcaa tttgggcggg gctactcacg cttgttgttc tcgaaattgt gctgggtatc
    20041 gataacctgg tcttcatcgc cattcttgct gacaaactgc cgccaaaaca acgcgataaa
    20101 gcgcgtttgc tggggttatc actggcgctg attatgcgtc tggggctgct gtcgctgatt
    20161 tcatggatgg tcacgctgac caaaccgcta tttaccgtca tggatttctc cttctccgga
    20221 cgcgacctga ttatgttgtt cggggggata ttcttgctgt tcaaagcaac aaccgaactg
    20281 catgaacggc tggaaaaccg cgatcatgat tccggccacg gtaaaggcta cgccagtttc
    20341 tgggtggtcg tcacacagat tgtcatcctt gacgccgtct tctcgttgga tgcggtaatt
    20401 actgcagtag ggatggttaa ccatctgccg gtgatgatgg cggcggtagt gattgcgatg
    20461 gcggttatgt tgctggcatc caaaccgctg acgcgattcg ttaaccagca ccccacggtg
    20521 gtggtgctct gtctgagctt cctgttaatg attggtctga gtctggtggc agaaggtttc
    20581 ggtttccaca ttccgaaagg ttacctgtat gccgcgattg gcttctcgat catcatcgaa
    20641 gtgtttaacc agattgcgcg tcgcaacttt attcgccacc agtcgacttt gccgctgcga
    20701 gcgcgtactg ccgatgccat cctgcgtttg atgggcggga aacgtcaggc caatgttcag
    20761 cacgatgccg ataacccgat gccgatgccg atcccggaag gtgcatttgc cgaagaagaa
    20821 cgttacatga ttaacggcgt actgacgctt gcgtcgcgtt ctctgcgcgg gatcatgacg
    20881 ccgcgcggtg aaataagctg ggttgacgct aatctcgggg tcgatgaaat ccgcgagcaa
    20941 ctgctctctt caccgcacag tctgttcccg gtatgtcgcg gtgaactgga tgaaatcatc
    21001 ggtattgtac gtgctaaaga actgctggtg gcgctggaag agggcgttga tgtggcggcg
    21061 attgcttcgg cgtctccggc gattatcgtc ccggaaaccc tcgatccgat caacctgttg
    21121 ggcgtgctgc gtcgtgctcg cgggagcttt gttatcgtga ccaacgagtt tggtgtggta
    21181 caaggtctgg tcacgccgct ggatgtgctg gaagccattg cgggtgaatt cccggacgct
    21241 gacgaaacgc cggaaatcat tactgatggt gacggctggc tggtaaaagg cggtacagat
    21301 ttgcatgcct tgcagcaggc gcttgatgtt gagcaccttg ccgatgacga tgatatcgcg
    21361 acggtcgcgg gcctcgtgat ctcggcaaat ggtcacattc cccgtgtggg cgatgtgatt
    21421 gatgtagggc cactgcatat caccatcatt gaagccaatg attatcgtgt tgatctggtt
    21481 cgcattgtta aagagcaacc ggcgcacgat gaagatgagt aagcattaac gtaacggcat
    21541 aatgggcgtg atatgtccat tatgccgggc gggcggcggt tggctgcccg ccagccattt
    21601 gggaaaatca cgtagcggca tcggctgtgc gtacaaatac ccttgcaaaa tatgcacgcc
    21661 atgacggcgc aaatacttcg cctgttcttg cgtttccacg ccctccgcca ccagttcaat
    21721 attcagtctt tgccccagcg cgatgatgat atcggtcacc gtcgagttaa ccgcgtcaga
    21781 acctatagct gcggtaaatg acttatcaat tttcagcatg tcaggacgta atgtttcaag
    21841 ccaggaaaat gaactgttgc cggtgccgaa gtcatcaatc gccagtttga cgtttttacg
    21901 atgtagctcg cgggcaatcc gataatcaac atccagtaag gcatcgcgtt cggtgatttc
    21961 gaggatcagt tgctgaatcg ggtgagcgct aaaccagtac tgattgagat cttttatcag
    22021 cacaccacgg cgaaaatggc tgggtgcgac gttaatgcca acatgaaact gactactcat
    22081 cgggaaaaca tggcgctgac gaatggtttc tgccatcaca tagcgggtca gtggcacaat
    22141 taaatgatgt tcttccgcga taggaataaa cacatccggt gaaatccagc cctgacgcgg
    22201 attgttccag cgcagcaaaa tctctacacc aatacattgc tggctgcgcg cattaagcaa
    22261 aggctgacag aacaattcga actcatgttg cgccaggccc agattgattt cgcgggaaaa
    22321 gctcatccgg taagcggtcg ccagccaggc gatgtagccc accagtaaac ttagcagcac
    22381 cgccagcggt aattgtgttg gaagatagtg ccatgccagc gccgtagccc ccggaccatt
    22441 aacgttaatg gtaaaaggaa agcgttgcga agaaacctga tagttttcat tgtcttcagg
    22501 ttgcggaagg gaatctacca gcccattacc atagagcaaa tgccgtttgt ccaccgtcag
    22561 gctggcgcta ctgatttgcg gcagttgtgg ctcaaggagc attgccgtca gtaagtcgat
    22621 gttaatcatc tccatgaccc cagcattgct actgcccgct gcaggcgtcc attgaatcaa
    22681 aaccggactg cctttaatca gggcacggtc gatcgtcagg cgtaaaagtg gttgcggtgc
    22741 aggaagttca gccagaatgt ccacgacggg cacattgcga taaccaaaaa tgctggagca
    22801 atagagtgtt ccgtcttgca ccaggccaat ggatcgcaca gtttggagtt ttgccgcctg
    22861 tttacgtaat ggcaaatggg cgacagagca gggcagacca atcagcggaa gcaagacttc
    22921 gctacctgcc tgtaggggaa gcagtacttt atctaattcc tctacagcgt gattggcgaa
    22981 ttgtactacc cgttgttgat ttaagttacg ctgtgaaata aattgcacgc tcagggtcga
    23041 agcgagcgta acgagtgcgc aaatcgtaca aacaatcatt cgattacggc gataggtttt
    23101 aatgatccgt tgtgcttttt gcatgcgggc caccctgtaa gcgtatggcg acaaacgcct
    23161 gacgcaacag tggaagtgta gtggggaata ttacagcaga cgagaaagcg ggtataaatt
    23221 cgcccatccg ttgcagatgg gcgagtaaga agtattagtc acactggact ttgattgcca
    23281 gaccaccgcg tgaggtttcg cggtatttgg cgttcatgtc cttaccagtt tcgtacatcg
    23341 tttcgatgac cttatccagc gagacgcgcg gtgcactggt gcggcgcaga gccatccgtg
    23401 cggcgttaat cgccttcaca gaggcaatgg cattacgctc aatgcacggc acctgaacct
    23461 gccctgcaac cgggtcgcag gttaaaccaa ggttgtgttc catgccaatt tccgccgcca
    23521 cgcaaacctg ttccgggcta ccgcccagca gttctgcaag acccgcagca gccattgaac
    23581 aggcaacacc cacttcgccc tggcaaccaa cttccgcacc ggaaatagag gcgttcattt
    23641 tatacaatgc accaatcgcg cccgctgcca taaagtaacg ggtatagatg tccgggctga
    23701 ccgattcaat aaagtggtca tagtaagcca gcactgccgg aacgataccg caggcaccgt
    23761 tggttggcgc agttaccaca cgaccaccgg cggcgttttc ttcgttaact gccagcgcaa
    23821 acatgtttac ccagtcaatg acattcatcg gatcgttaga cagtttatcg ctggaaacta
    23881 acatccggcg cagggcagac gcacgacgtg gcacgcgcag cgggcctggc agtacacctt
    23941 cggtgttcat cccgcgatcg atacatgcct gcatggtttg ccagacatgc gcgaaatact
    24001 cgtcgatctc tttcttgctg tgcagcgcca gttcgttctg catagcgaga ccagacagcg
    24061 aatagccggt ttcattacag tacgcgagca gttcggtggc agatttgaac ggatacggca
    24121 cgctcacttc gttggcagca tcctgaccaa agtgttcttc atcgacgata aaaccgccgc
    24181 cgatggaata ataagttttg ctgtagacga cttcatcgcc gttataggcg tggatttgca
    24241 taccgttttc atgcagcggc aggttgccgt tatgaaaacg catcccgttg tcgcgcggga
    24301 aatccacttc atgccgtccc tgtgccagca gcagacgttc gcgctcttct acgtcgcgaa
    24361 taaaaccggg aatactgtcg atatccacgg tggcaggttc gttacctgca agacccataa
    24421 taatggcgat atcggtgtgg tggcctttac ccgtcagcga cagtgaacca taaacgtcca
    24481 cggcaacgcg agtaacgcta tccagtaagc ctttttcgac cagatcatcg acgaactgtt
    24541 tacctgcctt cataggccct acggtatggg aagatgaggg accaatcccc accttaaaca
    24601 tgtcgaatag actaatcacg ataatactcc tgacaaggtg actggacttc cagtaacgaa
    24661 ctaataacag cgcatagtgt aagagggaac gcggcgactg gcttaactat tcacatgaat
    24721 taaactaatc aaaaaccgcg atttaactaa tattgcgatg attgtctcaa gtagtgtttt
    24781 gtagatgtaa agataagtat agtcagggtt tcacaccaat ttgcagcgcc agctcacgaa
    24841 ttatgcctgc ggtcattccc catacaaaat actgttcgta ccaggacagc catacccgat
    24901 gtgaatcgcc acggcggtag atatctaaag ggtgataacg acccagatgt aatgcctggg
    24961 cgagcggcat ttcaaacacc gccgagactt catcttcact ggcgcgatac ggcagatcgg
    25021 gcgggataat gccgaccact ggggttacct gatagccagt tacgctatcg acgggcggca
    25081 gcacgccgat aacttcaacg gcggaaggcg gtatagcgac ctcttcttca gcttcgcgca
    25141 gcgcggcggc gataactgat gcgtccgtgt catcgactgc acctccaggg aatgccactt
    25201 gtccagcgtg tttacgcaga tgaattgaac gctgagtcag caacaacccc ggttgcggtc
    25261 gacggacgat ggggattaac acggcagcct gacgatgatt tagggtttcc cggttaattt
    25321 gcgggcgcaa aagttgaaag cgcgataaaa aatcatcaag catcaggcta cggtattcca
    25381 cgtcttactt ctccagttgg cgtaatatct tattaacttt atcaaaagtt tcctgatatt
    25441 ccgcttcttc ctggctatcg gcgacaattc caccgcccgc agagcagtat atttgtccgt
    25501 taatggcagt cagagtgcgg atagtaatgc tggtatccat gttgccgcaa aagctcaaat
    25561 agccaatgct gccgcaccag gcattacgtc gctgcggttc cagttcgtcg ataatttcca
    25621 tagcccgtac tttcggagcc ccggttattg agccaccagg aaaagctgcg cgcagcagat
    25681 cgctggcgtg taactgttct ggtagttgtg ccgttatggt gctgaccaga tgatgcacgg
    25741 cagggaaggg ttccaccacg aacagctctg gtacttttac cgaacctgct acggcaacac
    25801 gaccgatatc attacgcatt aaatcgacaa tcatcagatt ttcggcacga tctttcgctg
    25861 agttcgccag tttttctgct tgtttgctat cttcctgagg atcgggcagg cgtggtagcg
    25921 tgcctttaat agggcgggtc tggatttcac tattatcaca aagaataaac cgctctggcg
    25981 aaaggcttaa aattgcaccc tgttcaagac gtaaaaaagc gctaaatggc gcgcggttgg
    26041 cctgattaag ctgaaggaat gcctgccatt catcgccaga ataggtcgca tgaaaacgct
    26101 gggcgagatt tacctgatag caatcaccgc tgtgcagata ttcctgtacc tggcgaaatt
    26161 tttcgccgta ctgctcgcgg gtcatattgg attgccagtc ggaagtaagc gtgaaatctt
    26221 cctgcggcga gaattgctgg ctttccagcc aggcccgacg ggcattgaca tcattatgac
    26281 tcagcaaaga aactgtatga cgctggtggt cgacaatgag cgcccaatcg tagataccca
    26341 ctgccatatc cggcagaacg atatcttgtt ccgcaatttc tggcagtgac tcaaaacggc
    26401 ggcccagatc gtagccaaac aaccccagtg cgccgccctg aaatggcaaa tcttcgttat
    26461 gcgttgggcg aatgtctgcg cgatccagca cctgctggag cacctgtagc gggtcatcag
    26521 tggtcgttgt gcgtttttcg ctttcactaa caacggtttc tttacctaaa gtggttaaag
    26581 tgcaaatcgg atcggcgacc acaatatcaa agcggctata cggatgatcg gcatagccgg
    26641 agtgtaaaag catcgcccac ggcaggtggc ttaagcggga gaaataaaat tcagcggcgt
    26701 cctgacgcca gggtaaagta atcacagcgg gagataacgt cttcatcagt cctgactcta
    26761 ctggctatgt gccgggtagc atgaaataat tagcgccaac aatttagcag gagttaacaa
    26821 tgtttgcagg tttaccttca ctcacccatg aacagcagca aaaagctgtc gagcggatcc
    26881 aggaactgat ggcacaaggg atgagcagcg gccaggcaat cgcgttggtg gcagaagagc
    26941 tgcgtgccaa ccattccggc gagcgaatcg tggcgcgttt tgaggatgaa gacgagtaac
    27001 acactaatcc gtattgattt cgttatacaa gccgatgatt atttgattgt gagaaaaagg
    27061 acgcttttta tatgtgatta aaagcgtcct gatccattta tgctgatgta aattttactc
    27121 agaccagtgc ttttagttct tcctgcatat atgttgaaaa gtattcagga tttttaatca
    27181 cagagcgttc acctgactcc attttttttg cccatccaac cattttttcc gtaaacccag
    27241 ggttccagcc ttcctggtca gctcgcgccg taatatcagc ggcactcgca ggtactccca
    27301 gttcttttaa atatttaaag attcccttcg ccgtgctttc attcatcgaa cttggtgttg
    27361 gcgctgatac atccatggca ccaatgaaat ccagggcttt atctattaca gcaggcatat
    27421 tcttatcctt aaaattaacc atgttatcga taaggctata tatgacctta
//
LOCUS       DAESHI010000049        27110 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.049, whole genome shotgun sequence.
ACCESSION   DAESHI010000049 DAESHI010000000
VERSION     DAESHI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27110
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.049"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..926
                     /locus_tag="KW011_002168"
     CDS             <1..926
                     /locus_tag="KW011_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBH7660492.1"
                     /translation="TSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKL
                     NDSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPV
                     NIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNL
                     TAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQ
                     MRIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGT
                     VNMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(969..1829)
                     /gene="rsmI"
                     /locus_tag="KW011_002169"
     CDS             complement(969..1829)
                     /gene="rsmI"
                     /locus_tag="KW011_002169"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBH7660493.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1893..3929
                     /gene="lpoA"
                     /locus_tag="KW011_002170"
     CDS             1893..3929
                     /gene="lpoA"
                     /locus_tag="KW011_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HBH7660494.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3887..4282
                     /locus_tag="KW011_002171"
     CDS             3887..4282
                     /locus_tag="KW011_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBH7660495.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4302..4892
                     /gene="diaA"
                     /locus_tag="KW011_002172"
     CDS             4302..4892
                     /gene="diaA"
                     /locus_tag="KW011_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HBH7660496.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4902..5477
                     /gene="yraP"
                     /locus_tag="KW011_002173"
     CDS             4902..5477
                     /gene="yraP"
                     /locus_tag="KW011_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HBH7660497.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5591..6631)
                     /locus_tag="KW011_002174"
     CDS             complement(5591..6631)
                     /locus_tag="KW011_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBH7660498.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6704..7339)
                     /locus_tag="KW011_002175"
     CDS             complement(6704..7339)
                     /locus_tag="KW011_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBH7660499.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7467..7985
                     /gene="yhbO"
                     /locus_tag="KW011_002176"
     CDS             7467..7985
                     /gene="yhbO"
                     /locus_tag="KW011_002176"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HBH7660500.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7965..8408)
                     /locus_tag="KW011_002177"
     CDS             complement(7965..8408)
                     /locus_tag="KW011_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HBH7660501.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8459..8761
                     /gene="yhbQ"
                     /locus_tag="KW011_002178"
     CDS             8459..8761
                     /gene="yhbQ"
                     /locus_tag="KW011_002178"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HBH7660502.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8748..9251)
                     /locus_tag="KW011_002179"
     CDS             complement(8748..9251)
                     /locus_tag="KW011_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBH7660503.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9245..9769)
                     /locus_tag="KW011_002180"
     CDS             complement(9245..9769)
                     /locus_tag="KW011_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HBH7660504.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9978..10973
                     /locus_tag="KW011_002181"
     CDS             9978..10973
                     /locus_tag="KW011_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBH7660505.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10982..11860
                     /locus_tag="KW011_002182"
     CDS             10982..11860
                     /locus_tag="KW011_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBH7660506.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDKLGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12066..13073
                     /locus_tag="KW011_002183"
     CDS             12066..13073
                     /locus_tag="KW011_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HBH7660507.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13191..14435)
                     /gene="mtr"
                     /locus_tag="KW011_002184"
     CDS             complement(13191..14435)
                     /gene="mtr"
                     /locus_tag="KW011_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HBH7660508.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14589..16478)
                     /gene="deaD"
                     /locus_tag="KW011_002185"
     CDS             complement(14589..16478)
                     /gene="deaD"
                     /locus_tag="KW011_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HBH7660509.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16471..16551)
                     /gene="yrbN"
                     /locus_tag="KW011_002186"
     CDS             complement(16471..16551)
                     /gene="yrbN"
                     /locus_tag="KW011_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HBH7660510.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16658..17542)
                     /gene="nlpI"
                     /locus_tag="KW011_002187"
     CDS             complement(16658..17542)
                     /gene="nlpI"
                     /locus_tag="KW011_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HBH7660511.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17651..19786)
                     /gene="pnp"
                     /locus_tag="KW011_002188"
     CDS             complement(17651..19786)
                     /gene="pnp"
                     /locus_tag="KW011_002188"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBH7660512.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20033..20302)
                     /gene="rpsO"
                     /locus_tag="KW011_002189"
     CDS             complement(20033..20302)
                     /gene="rpsO"
                     /locus_tag="KW011_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBH7660513.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20451..21395)
                     /gene="truB"
                     /locus_tag="KW011_002190"
     CDS             complement(20451..21395)
                     /gene="truB"
                     /locus_tag="KW011_002190"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBH7660514.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21395..21796)
                     /gene="rbfA"
                     /locus_tag="KW011_002191"
     CDS             complement(21395..21796)
                     /gene="rbfA"
                     /locus_tag="KW011_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBH7660515.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21960..24632)
                     /gene="infB"
                     /locus_tag="KW011_002192"
     CDS             complement(21960..24632)
                     /gene="infB"
                     /locus_tag="KW011_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBH7660516.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24657..26144)
                     /gene="nusA"
                     /locus_tag="KW011_002193"
     CDS             complement(24657..26144)
                     /gene="nusA"
                     /locus_tag="KW011_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBH7660517.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26172..26624)
                     /gene="rimP"
                     /locus_tag="KW011_002194"
     CDS             complement(26172..26624)
                     /gene="rimP"
                     /locus_tag="KW011_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBH7660518.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26831..26907)
                     /locus_tag="KW011_002195"
     tRNA            complement(26831..26907)
                     /locus_tag="KW011_002195"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26871..26873),aa:Met,seq:cat)
BASE COUNT         6424 a         7518 c         6809 g         6359 t
ORIGIN      
        1 ttacctctgc aacgctaagt agcactaact ggcaatacgc ctgttcctgc tctgcgggta
       61 aggcagttaa acttgtctat atggtcagcc ccgtacttac caccactgga catcagacag
      121 gatattacaa actcaatgac agcctggata ttaaaaccac attacaggca aacgacattc
      181 caggactcac aaccgaccag gttgtctctg ttaacacccg attcacacag ataaaaagca
      241 gcaccgtata ttctgctgca acccaaacgg gtgtttgcca gggtgacacg tctcgttatg
      301 gacccgttaa tattggtgca aataccacct ttaccctgta tgtcaccaag ccatttctcg
      361 gctcgatgac cattccgaaa acggatattg ccgtcattaa aggcgcgtgg gtcgatggaa
      421 tgggaagccc gtctacaggt gacttccatg atttagtcaa gttatcgatt cagggaaatc
      481 tcaccgcccc acagtcgtgc aaaattaatc agggcgatgt tattaaggtt aattttggat
      541 tcatcaatgg tcagaagttt accacccgca atgccatgcc agacggtttt actccagtag
      601 actttgatat cacttatgac tgtggtgata cttcaaagat taaaaactcg ttgcaaatgc
      661 gcatcgacgg tacaactggg gtagtagacc agtacaacct ggtcgccagg cgaagaagtt
      721 cagacaatgt gcccgatgtc ggtattcgta ttgaaaatct cggcggcgga gttgcaaata
      781 ttccttttca gaacggtatc cttcccgttg atccttccgg gcatggcacc gtcaatatgc
      841 gcgcctggcc agttaatctg gtcggtggtg agctggaaac aggaaaattt cagggcaccg
      901 ccaccattac cgtcatggtg cggtaaaaga agacgttgcg ttttgattaa cgcaacgctt
      961 cggcactgtt acccctgctg ctccagcgca tacttataca gcgcattttt cttcacgcca
     1021 tgaatttctg cggccagcgc cgccgctttt ttcagcggca gttctgcctg tagcagcgcc
     1081 agcgtgcgca gggcatcggc gggtaagtct tcttcctgtg ctttatgacc ttcgacaatc
     1141 agcaccattt cgcctttgcg acggttttca tcttccttta cccacgccag cagctcgcca
     1201 acgggcgcgc cgtgaatggt ttcccaggtt ttggtcagct cacgcgccag aaccacgtag
     1261 cgggattcgc ctaataccgc aacgatatct tccaggctat ctaacagacg gtgggtagat
     1321 tcataaaaaa tcagcgtgcg cggctccgct tcaatggctt ttagcgcatc acggcggcct
     1381 tttgatttgg caggtaaaaa gccttcgtaa cagaaacggt cagagggtaa acccgcggcg
     1441 cttaacgcag tgatagcagc acacggtccg ggtagcggca ccacacgaat ccccgcttca
     1501 cggcaggtac gcaccagatg gtagccagga tcgttaatta gcggcgttcc ggcatcggaa
     1561 accagcgcaa tgttttgccc ctcttgcagc ttcgccagca gcgtttcggc tttttgttgt
     1621 tcgttatggt cgtgcagcgc aaacaaccgg gcattaatcc caaaatgttg cagcaataaa
     1681 ccggtgtgac gagtatcctc ggcggcaatc agatcaacgg cctgtaatac ctctaacgca
     1741 cgctgggtga tatccgccag attgccgatt ggcgtcggta caatgtaaag ctggccctga
     1801 gaattatccg ccgattggtg ttgtttcatt gtgtcgtccg tattgccgat ttaatattga
     1861 gcattgcgta aaaaaatatc actggataca ttatggtacc ctcaacattt tctcgtttga
     1921 aagccgcgcg ttgtctgcct gttgttctgg cagccctgat tttcgccggt tgtggcaccc
     1981 atactcccga tcagtccact gcttatatgc agggcacggc gcaggctgat tctgcctttt
     2041 atcttcagca gatgcagcaa agctctgatg ataccaggat caactggcaa ttactcgcca
     2101 ttcgtgcact ggtgaaagaa ggtaaaaccg ggcaggcggt tgagttgttt aaccaactac
     2161 cgcaagaact gaacgattct cagcgtcgcg agaaaacact gctggcggta gagattaaac
     2221 tggcgcagaa agattttgct ggcgcgcaaa acttgctggc gaaaatcaca cctgccgatt
     2281 tagaacaaaa ccagcaagcg cgttactggc aggcaaaaat cgatgccagc caggggcgtc
     2341 cttccattga tttactgcgc gcgttaattg ctcaggaacc gctgctcggc gcgaaagaaa
     2401 aaaagcagaa tattgatgcc acctggcagg cgctctcctc catgactcag gaacaggcga
     2461 atacgctggt gatcaacgcc gacgaaaata ttctgcaagg ctggctggat ctgcagcgcg
     2521 tctggtttga taaccgtaac gatcccgaca tgatgaaagc cgggatcgcc gactggcaaa
     2581 aacgttatcc gaacaatccg ggcgcgaaaa tgctgccaac gcagttggtt aacgtaaaag
     2641 cgtttaaacc agcctcgacc aacaaaatcg ccctgctgtt gccgctgaat ggccaggcag
     2701 cggtatttgg tcgcaccatt cagcaaggct ttgaagcggc gaaaaatatc ggcactcagc
     2761 cagtggcggc tcaggtagct gccgcacctg ccgcagacgt agctgaacaa cctcagccgc
     2821 aaaccgtgga tggcgttgcc agcccggcac aagcctcggt tagcgatctg accggtgatc
     2881 agcctgcggc ccagccggtg cctgtaagcg ccccggcgac aagcaccgca gcggtaagcg
     2941 cacccgcaaa tccatccgca gagctgaaaa tctacgatac ctcatcacaa ccacttagcc
     3001 agatcttaag ccaggttcag caggatggcg cgagtattgt ggttggtccg ttgctgaaaa
     3061 ataacgttga agagttgctg aagagcaaca ccccgctgaa cgtgctggca ctgaaccagc
     3121 cagagaatat cgaaaaccgc gtcaatattt gttacttcgc gctttcacct gaagacgaag
     3181 cgcgcgatgc agcgcgtcat attcgtgacc agggtaaaca agcgccgctg gtgctgatcc
     3241 cacgcagttc attgggcgat cgcgtagcca atgcgtttgc gcaagagtgg cagaaactgg
     3301 gcggcggcac cgttctgcaa caaaaatttg gttccaccag cgaattacgc gcgggtgtta
     3361 acggcggttc tggtatcgct ttaacgggta ccccgattac tcccagagcg acaaccgact
     3421 ccggcatgac gaccaacaat ccaacgctgc aaaccacgcc aaccgatgac cagttcacca
     3481 ataatggcgg tcgtgtcgat gcggtgtaca ttgtggcaac gccgggtgaa atcgctttta
     3541 tcaaaccgat gatcgccatg cgtaacggta gccagagcgg tgcaatgctg tacgccagct
     3601 cccgcagcgc acaaggcacc gcaggcccgg atttccgtct ggagatggaa ggtttgcagt
     3661 acagcgaaat cccgatgctg gcgggcggta atctgccgtt aatgcagcag gcactcagcg
     3721 cggtgaataa cgattattca ctggctcgca tgtatgcgat gggcgtcgat gcctggtcgc
     3781 tggcaaatca tttctcacaa atgcgccagg ttcagggttt tgaaatcaac ggtaataccg
     3841 gaagcctgac ggcaaacccg gattgcgtga ttaacaggaa gttatcatgg ctacagtacc
     3901 aacaaggtca ggtagtcccc gtcagttaac caccaaacag accggcgatg cgtgggaagc
     3961 acaagcgcgt cgctggctgg aaggcaaagg actgcggttt atcgccgcta acgtgaacga
     4021 gcgtggcggc gagatcgatc tgataatgcg tgaaggccgg accaccgttt ttgtcgaggt
     4081 acgctatcgc cgctctgcgc tttatggcgg cgcggcagcc agtgtgaccc gcagcaaaca
     4141 acacaaatta ttacagactg cccgcttgtg gctcgcgcgt cataatggga gttttgatac
     4201 tgtggattgc cggttcgatg tggtagcctt caccgggaat gaggttgagt ggattaagga
     4261 tgcctttaat gaccactcat aattaaggtt taaggattag cgtgcaagaa agaattaaag
     4321 cttgcttcac tgaaagcatt caaactcaaa ttgcggcggc agaggcgctt ccggatgcca
     4381 tctcccgtgc agccatgacg ctggttcagt ctctgctcaa tggcaacaaa atcctctgtt
     4441 gtggtaatgg aacttccgct gccaatgcac agcattttgc tgccagcatg atcaaccgtt
     4501 tcgaaacgga gcggcccagc ttacctgcca ttgcactaaa tactgataat gttgtcttaa
     4561 cggcgattgc caacgatcgc ttacatgatg aagtgtatgc aaaacaggtg cgggcgctgg
     4621 gtcatgcggg agatgtattg ttagccattt ccacccgtgg caacagccgc gatattgtta
     4681 aagcagttga agccgccgtt acgcgtgata tgaccattgt ggcattgacc ggctatgacg
     4741 gcggcgagct tgcaggtttg ttagggccac aggatgtgga gatccgcatt ccttcgcatc
     4801 gtagtgctcg cattcaggaa atgcatatgc tgacggtaaa ttgcctgtgc gatctgatcg
     4861 ataacacgct tttccctcac caggatgatt aaggagaata catgaaggca ttatcgccaa
     4921 tcgcagtcct tatttccgcg ctgctgttgc aaggttgtgt tgccgctgcc gtagtgggta
     4981 ccgctgctgt gggtaccaaa gccgcaactg acccacgcag tgtcggcacc caggtggacg
     5041 atggtaccct ggaagtgcgc gtgaacagcg cattgtcgaa agacgaacag attaagaaag
     5101 aaacccgtat taatgtcacg gcgtatcagg gcaaagtgct gctggttggg cagtcaccaa
     5161 acgctgaact ttcggctcgc gccaaacaga ttgctatggg cgtagacggt gccaacgaag
     5221 tgtataacga gattcgtcag ggccagccga ttggtctggg cgaagcatct aacgatacgt
     5281 ggatcaccac caaagtgcgt tcgcagctct taaccagcga cctggtgaaa tcgtccaacg
     5341 tgaaagtgac caccgaaaac ggtgaagtgt tcctgatggg gctggtgact gaacgtgaag
     5401 cgaaagcggc ggcagatatt gccagccggg tgagcggcgt aaagcgggta accacagcgt
     5461 ttacgtttat taaatagcag ccccttgtaa tgcctgatgc gacgcttgcc gcgtcttatc
     5521 aggcctacaa cgatgcaaac cgtaggtcgg ataaggcgtt tacgccgcat ccgacatgtt
     5581 ttccctcaaa tcagaataac agcgccagcc cgccgacaat cacgcctgac actgccacca
     5641 tcgcccccgt cagccataag gctttcgccg ggaacgcttt gcgcagcata atcagtgacg
     5701 gcaaactcac tgccgggagc gtcatcaaca atgccagcgc cggagcggtt cccatacctg
     5761 ccagcatcat cgtttgtaca atcggaattt ctgctgccgt gggaatgaca aacaagcatc
     5821 ctgctaccgc catcgccacc acccacatca ggctgttatc gacagcacca tcggcatggg
     5881 ggaataacca gacgcgtgcg gcacccaaca ccagtactgc aaggatgtaa accgggatcg
     5941 tactccagaa aagtgtccat agcgccctgc cccagcggct aaaaaaaccg ctctgtgctt
     6001 ccggtatgtc aatttcgacc ggtgcctgcg tttgcggtgt ttcacgcacc catttttgca
     6061 ccagcgtcgc aatcagcaac accatcacca gcccggccac cagacgaatc gccgcaaaac
     6121 cccagccgag gacaaacccc ataaacacca gcgtcgccgg gtttaacacc ggattgccca
     6181 tccagaatgc cagcgcaccg cccatcgata cctgttggcg acgcattccc gccgcgaccg
     6241 gagccgcaca gcaggtacac atcatgcccg gcagcgaaaa cagcgttccc agcagcgtgc
     6301 cgcgaaagcg cgattgtcca agcgtacgca acaaccagtc acgcgggatc agcacctgaa
     6361 tcaacgaacc gaggatcacc cccagcaccg ccgctttcca taccgcgagg aaatagatca
     6421 tcgcgtaatc caacgccgcc tgccacggat tagcatccgc ctgcgcaagg atagatttac
     6481 cgatactgtg ggtttcggca gcagtaaacg ctttgccgta gtaaggttgc catttcacat
     6541 accagagacc ggcaatgaca acgagaaaga aaagcgcggg tttccaccac tgaatgggcg
     6601 ttgccgcctg agatgaagac tgaccagtca tagcattccc caggaataag ttatgataat
     6661 tgagcgcgtg aatattacgc tcactatcaa ttcttgggaa taattattca gctcttttgc
     6721 gtaattctga agagcttaaa atagtcacgc cttcatgctc aggccgcatc gcctctgcca
     6781 gcatgacccg cgccacgtct ctcgcatcaa tggatttcca gttaccgggc agtaaacgaa
     6841 acagcggcgc aaaaagcgtt tcgttcatcc gctgtttgct acgatcgccc agcaacatcg
     6901 acgggcgagc aatggtcaat ttcggccagt tctgggcaat taatgcttct tccatctccc
     6961 ctttgacgcg gttatagaaa aacggcgagt gggcattggc acccatagca ctgaccacca
     7021 gcatatgctg cgcgcccagt cgccgcccgg ttaatgcggt atccactacc agcgtgtaat
     7081 cggcatgaat aaacgcctct ttgctccccg cttctcgccg cgtggtgccg agacaacaaa
     7141 acacaatgtc gatgggttcg gtgacctgcg ccaacgcgtc actcagttgc ggatcatggg
     7201 gattaaacac cccgggcata tcgcccaacg gacgtcgcgt cggcgcggct atggcgttaa
     7261 ctttcggttc gttaatcaac atccgcagca ggtgaccgcc caccagcccc gttgcgcctg
     7321 taatcagtac ctgactcatc ttcgctcctt tacagaattg tctgccttgc gctccacggc
     7381 tcatgcacca ggcttaatag accatgaggt aattatcccc gattgtgtga aattcgcctc
     7441 atccaatgca acaacgcgga ggaagcatga gtaagaaaat tgccgtttta atcagtgatg
     7501 aatttgagga ttcagaattt acttcacccg cagacgagtt ccgtaaagcc ggacacgaag
     7561 tgattaccat tgaaaaacaa gcgggtaaaa cggtgaaagg caaaaaagga gaagccagcg
     7621 tgaccatcga taaatccatc gatgaagtga cgccagcgga gtttgatgcc ctgctgctac
     7681 cgggtggtca ttcaccggat tatctgcgtg gcgacaaccg ttttgtcacc tttacccgtg
     7741 attttgtgaa tagcggcaaa cctgttttcg ccatctgtca cggtccgcag ctgctgatca
     7801 gcgccgatgt gattcggggg cgcaaactga ccgcagttaa accgatcatt attgatgtta
     7861 aaaatgcggg cgcggaattt tacgatcagg aagtcgtggt tgataaagat cagctggtta
     7921 ccagccggac accggacgat ctgccagcgt ttaaccgcga ggcgttacgc ctgctcggtg
     7981 cctgagtcgc gtagccaaat cattttttta ccaaagccca gcgtgttgtc agtgaatttg
     8041 atttcatcga gccggatttc ccacaccggg gccgacagca ttctggcaac cggaaagcga
     8101 cgattgtacg cctggcgcgc gaggtcgctt tcctcacctt ccagcctgcg gatctcacct
     8161 ttaaactgca cgccgcgaat taacgctacc gttttcggct gaccgtttac cgttccggca
     8221 accgcagcct gcggcccact catctgcgcg tggcgcgttt tttcttccgt caaaatgtag
     8281 aaggcgactt tctgcgcatc aaacagataa aaagcattag cgcaccacag ttccccttcc
     8341 tgctgcacac accaggtgac aacatgttgt tttgccaacc aacggctgat ggtgatgagt
     8401 gtttccattg ctgctctctt ttatactgtg ggcctgacct taacatgcga cgcagacgat
     8461 gacaccctgg tttctttact tgatccgtac cgccgacaat aagctttata ccgggatcac
     8521 cacggatgtc gaacgccgct atcagcagca ccaaagcggc aaaggggcga aagcactgcg
     8581 cgggaaagga gaactgacgc tggcgttttc tgcgccagtg ggcgatcgtt cgctggcgtt
     8641 acgggcggaa tatcgcgtta agcaactgac caaacggcaa aaagagcgtc tggtagcgga
     8701 aggcgcaggg tttgcagagc tgttaagcag tctgcaaacc ccggagatta aaagcgattg
     8761 aaatgctcgt gatactcaac cagaccggtt acgccattca gcgcgtcatc cgctaaacga
     8821 tgtacctgga aggcgctttc agtgcccggc cagcggcaac gcagatcgtg atgcgccgcc
     8881 agttcaaagc cgaaacgact gtacagcgcc ggatcgccca gcgtcaccac tgcggcatag
     8941 ccgaactcat taagcgaatc gagtccttca tagaccagtt ggcgtgccag cccctgtcca
     9001 cggtattttt catcaaccgc caacggtgcc atgccgaccc attgcaggtc ttcgccctgc
     9061 acatcaaccg gactaaatgc cacatagcca atgacctgac cttcgtcatc tgtcgccacc
     9121 agccccagcg tcagaaagcc atcttcacgc agatcgtgaa ccagcttcgc ttccgcatca
     9181 ctttcgaatg agcgacgcag cagggcatca atacccggcg catcaatggg aatttctact
     9241 cgaattagca tggttcacct accgatgtct gtttggtttc aggcgcggtt ttcattcccg
     9301 cctcaacaaa atccgccagt tgcagcagca tcatgcgtaa ggctttcggc atttgctcca
     9361 gttcaatggc gtccatcagg tttttcacat acagccccag ctccgtatcg ccttcaatca
     9421 ccagccgacg ctggaagaag agcgtatccg gatcttgttt acgcgccgca atcatcagca
     9481 gatcgctggc gtcggcacta aaactcacat cagcttgcgc gttctggctg accaccagtt
     9541 tgccattcac caccgaggta aaccattgca ggtcaatatc acgcacatga atacttaacc
     9601 agcggccttc aagaaactcc agctcaccat catccagcgc ctgacggaat tgccagctta
     9661 agacctgctc aagaacctgg cgttttagcg caaatggcgt cagttttacc ggtacactca
     9721 acagagatgg ccccaaatgc acaatacggg aacgcagttt atccaacacg agttttactc
     9781 cctgtttcaa caatcatcct attttgccat atcagaaaaa taacatagcg gtataaatca
     9841 acaattccat atgaaattgc tgctaccacc aatacaactt taactgcctt aaatcaaaaa
     9901 ttgtcgcagc aaggttaact aaaatcccag ttcgttaaca tttttgcgtt ttgaaagcgc
     9961 aaccttcagg aaaaattatg gagctgctct gccctgccgg aaatctcccg gcgcttaagg
    10021 cggccatcga aaacggcgca gatgctgttt atatcgggct aaaagatgat actaatgccc
    10081 gtcacttcgc cggccttaac tttaccgaga aaaaattgca ggaagcggtg agttttgtcc
    10141 atcaacatcg ccgcaaactt cacatcgcga ttaacacttt tgcgcatccg gacggttacg
    10201 cccgttggca gcgcgccgtg gatatggcgg cgcagctggg tgccgacgcg ctgatcctcg
    10261 ccgacctcgc catgctggag tacgccgccg agcgttaccc gcatattgag cgtcatgtgt
    10321 cagtgcaggc ttcggcgacc aatgaagagg cgattaactt ttatcatcgc cattttgatg
    10381 ttgcccgcgt ggtgctgccg cgcgtgttgt cgattcatca ggtgaaacag ctggcacggg
    10441 tcacacctgt cccgctggaa gtctttgctt ttggcagcct gtgcattatg tcggaaggtc
    10501 gttgctatct gtcgtcgtat ctgacgggtg agtcgcccaa caccgtgggc gcgtgttctc
    10561 cggcccgttt cgtgcgctgg caacaaacgc cgcaggggct ggaatcccgc ctgaacgaag
    10621 tgctgatcga ccgttatcag gatggcgaaa acgcaggtta tccgacgctg tgtaaagggc
    10681 gttatctggt ggacggcgag cgctatcacg cgctggaaga accaaccagt ctcaataccc
    10741 tggaactgct gccggagtta atggcggcga atattgcttc ggtgaaaatt gaaggccgcc
    10801 agcgtagccc ggcgtatgtc agccaggtgg cgaaagtctg gcgtcaggct atcgaccgtt
    10861 gtaaggccga tccgcaaaac ttcgtaccgc aaagcgcgtg gatggagacg ctcgggtcga
    10921 tgtccgaagg cacgcagacc acccttggcg cgtatcaccg taaatggcag tgagaagagc
    10981 aatgaaatat tccttagggc cagtgctgtg gtactggcca aaagaggcgc tggaagaatt
    11041 ttatcagcag gccgccacca gcagcgccga cgtgatttat cttggtgaag cggtatgcag
    11101 caagcgccgg gcaaccaaag ttggcgactg gctggagatg gcaaaatcgc tcgccggaag
    11161 cgggaagcag atagtgctct ccacgctggc gctggtgcag gcatcctctg aactgggcga
    11221 actgaaacgc tatgttgaga acggtgagtt tctgattgaa gccagcgatc tcggcgtggt
    11281 gaatatgtgc gctgaacgca aactgccgtt cgtcgccggg cacgcgctga actgctacaa
    11341 cgcggtgacg ctgaaaatat tgctcaaaca gggcatgatg cgctggtgta tgccggtgga
    11401 gctttcccgc gactggctgg tgaatctgct taatcagtgc gataagttgg ggattcgcaa
    11461 ccagtttgaa gtggaagtcc tgagctacgg tcatctaccg ctggcctact ccgcccgctg
    11521 ctttaccgcg cgttcggaag accgcccgaa agatgagtgt gaaacctgct gcattaagta
    11581 tccgaacggg cgcaacgtgc tgtcgcagga aaaccaacaa gtgtttgtac tcaatggcat
    11641 tcagaccatg agcggctacg tttacaacct cggtaacgag ctgacatcca tgcagggatt
    11701 ggttgatgtg gtccgcctgt caccgcaggg tactgacact ttcgcgatgc tcgacgcctt
    11761 ccgcgctaat gaaaatggcg ctgcgccact gccgttgacg gcaaacagcg actgtaacgg
    11821 ctactggcgg cggctggctg ggctggagct gcaagcctaa gtaaatatgc tcgtcatact
    11881 tcaagttgca tgtgctgcat ctgcattcgt tcaccccagt cacttactta tgtaagctcc
    11941 tggggattca ctctcttgtc gccttcctgc aactcgaatt atttagagca cagctcactt
    12001 tgttaacaac tttaactact ctttaatgca gtattaaaga ttaatcggta acaaagtgag
    12061 ctgttatgac tgataaaacc attgcgtttt cgctactcga tctggccccc attcccgaag
    12121 gttcttcagc gcgagaagca ttctcccact ctctcgatct cgcccgtctg gctgaaaagc
    12181 gcggctatca tcgctactgg ctggcagaac accacaatat gactggcatt gccagtgctg
    12241 ccacgtcggt attgattggc tatctggcgg cgaataccac cacgctgcat ctggggtctg
    12301 gcggcgtgat gttgcctaac cactcaccgt tggtcattgc cgaacagttc ggcacgctca
    12361 atacactcta tccggggcga atcgatttgg ggctgggtcg tgcgccaggt agtgaccaac
    12421 ggacaatgat ggcgctgcgt cgccatatga gcggcgatat tgataatttc ccccgcgatg
    12481 tcgcggagct ggtggactgg tttgacgccc gcgatcccaa tccgcatgtg cgcccggtac
    12541 caggctatgg cgagaaaatc cccgtgtggt tgttaggctc cagcctttac agcgcgcaac
    12601 tggcggcgca gcttggtctg ccgttcgcgt ttgcctcaca cttcgcgccg gatatgttgt
    12661 tccaggcgct gcatctttat cgcagcaact tcaaaccgtc ggcacgactg gaaaaaccat
    12721 acgcgatggt gtgcatcaat attatcgccg ccgacagcaa ccgcgacgct gaatttctat
    12781 ttacctcaat gcagcaagcc tttgtgaagc tgcgccgtgg cgaaaccgga caactgccgc
    12841 cgccgattca aaatatggat cagttctggt caccgtctga gcagtatggc gtgcagcagg
    12901 cgctgagtat gtcgttggtg ggtgataaag cgaaagtgcg tcacggcttg cagtcgatcc
    12961 tgcgcgaaac cgacgccgat gaaattatgg ttaacgggca gattttcgac caccaggcgc
    13021 gcctgcattc gtttgaactg gcgatggatg tgaaggaaga gttgttggga tagtgtgtct
    13081 taacgcggga agccttatcc gagctggcaa cgctgtccta cgtagacctg ataagcgaag
    13141 cgcatcaggc attgtgtagg cagcagaaat gtcggataag gcaccgctga ttactgatac
    13201 accggcagca aattaaagct cgataagata tggaccagtg cgttgccaac gccaaacacc
    13261 agaatcagcg taatcatcgg cttgccgccc cagacacgga atttcgggct gccaaagcgt
    13321 ttacgcgatg cgcgggctaa cagcgccgga acaattgccg cccagatggt agctgctaaa
    13381 ccagcataac caatggcgta caggaatccg ttcgggaaca gtagaccacc cacaacaggc
    13441 ggggcaaagg tcagcaatgc cgttttcaag cgccccaggg ctgagtcatc gaaaccaaac
    13501 agatctgcca gatagtcaaa caaacccagc gttacgccaa ggaacgaact cgctaccgca
    13561 aagtttgaga acacgaccag cagaaggtcc aggctacggc tgttcagcac gccgcttaat
    13621 gcctgtacca gcacatcaat attaccgccc ttctctgcaa taccgataaa ctccggacgc
    13681 gggatgttac ccatcgtcgc cagcaaccag atggtataca gcgccagtgc catcagcgta
    13741 ccgtacacca ggcatttcac gatggttttc ggatctttgc cgtaatactt catcaggctc
    13801 ggcacgttac cgtgataacc aaacgatgcc agacagaacg gcagggtcat cagcaaatac
    13861 ggcgcataag atgcattgct ttcggcgacg ttgaacaatg tcgcaggctg cacatgcccc
    13921 agtaggctac cgaaggtgag gaagaaggta atgactttcg cccccagtac aatcgccgtc
    13981 atgcggctga cggccttagt actcaaccac accacaaacg ctaccagcag tgcaaaacca
    14041 aaacccgccg cccgtgccgg gacgtttagc gacatctctg cgaaggtgtg atgcagaatt
    14101 gaaccactgg cagaaatata ggcataggtc aggatataga gcacaaaggc aatggaaata
    14161 ccgttgacca cgttccagcc tttgcccagc aaatctttgg tgatggtgtc aaaactcgaa
    14221 ccgattcggt aattcaggtt agcttccaga atcatcaagc cggaatgcag catacagaac
    14281 caggtaaaga tcagcgccgc cattgaccag aaaaaccacg ccccggacat gaccactggc
    14341 agagaaaaca tccctgcgcc aataatggtg ccgccgataa tcaccacgcc gccaagcagc
    14401 gacggtgacg tttgggtggt ggttagtgtt gccatgagag cttctctcca gtgaaaaata
    14461 gtgcgactgc gttgttatgc attgcactgt accagtacac gagtacaaaa gacagaaaaa
    14521 aagccccgat ggtaaaaatc ggggctgtat atattatttt acagattgtg ttcgctgttc
    14581 agcgatgatt acgcatcacc accgaaacga cgacgaccgg tagagtcatc acgacgcgga
    14641 gcgcggcctt cacgacgttc gccgctaaaa cgacgaccat caccacggcc accttcacgg
    14701 cgttcaccgc tgaagttacg accgccttca cgacgttcgc caccgaaacc acgaccaccg
    14761 ccacgacgct caccgccagt atgcggctgt gcatcgccca gtaactgcat gttcatcggc
    14821 ttgttgagaa tgcgagtgcg cgtaaagtgt tgcagcactt cacccggcat acctttcggc
    14881 agttcgatgg tggagtgaga agcaaacagc ttgatgttac caatgtaacg gctactgatg
    14941 tcgccttcgt tagcaatcgc accaacgata tgacgaactt caacaccatc atcgcggccc
    15001 acttcaatgc ggtacagctg catatcgcca acatcacgac gttcacgacg cggacgatct
    15061 tcacggtcac cacgcgggcc acggtcgtta cgatcgcgcg gaccacggtc atcacggtca
    15121 cggaattcac gtttcggacg catcggcgca tctggcggta cgatcagagt acgttcaccc
    15181 tgtgccattt tcagcagtgc cgcagccaga gtttcgagat ccagctcttc accttctgca
    15241 gtcggctgaa ttttgctcag cagtgcgcgg tattgatcca gatcgctgct ttccagctgc
    15301 tgctgtactt tagcggcgaa tttttccaga cggcgtttgc ccagcagttc tgcgttcggc
    15361 agttctactt ccggaatagt cagcttcata gtacgttcaa tgttgcgcag cagacgacgc
    15421 tcgcggttct caacgaacag cagcgcgcgg ccagcacgac ccgcacgacc ggtacgaccg
    15481 atacggtgaa cgtaagactc agaatccatc gggatatcgt agttaactac caggctgata
    15541 cgctcaacgt ccaggccacg ggctgcaacg tcggtcgcaa tcaggatgtc cagacgacca
    15601 tctttcaggc gttccagtgt ctgttcacgc agcgcctggt tcatgtcacc gttcagcgcg
    15661 gcgctgttgt agccgttacg ctcaagagct tcagccactt ccagagtcgc gtttttggta
    15721 cgaacgaaga taatcgccgc atcaaaatct tccgcttcca ggaaacgtac cagtgcttcg
    15781 tttttgcgca taccccagac agtccagtag ctctggctga tgtcaggacg ggtagtcacg
    15841 ctggactgaa tgcgcacttc ctgcggctct ttcataaagc ggcgggtaat gcgacgaatc
    15901 gcttccggca tggttgcaga gaacagagcg gtctgatgac cttccgggat ctgcgccata
    15961 atggtttcaa cgtcttcgat gaagcccatg cgcagcattt cgtcagcttc atccagaacc
    16021 agaccgctca gtttagagag gtccagagtg ccacgtttca ggtggtccag cagacggccc
    16081 ggagtaccga caacgatctg cggcccctga cgcagggcgc gtaattgcac gtcataacgc
    16141 tggccgccgt acagagcaac cacatttacg ccgcgcatgt gtttagagaa atccgtcatt
    16201 gcttcagcaa cctgtaccgc cagttcgcgg gtcggtgcca gcaccagaat ctgtggtgct
    16261 ttcagctcag gatcaagatt ctgcaacaga ggtaaagaga atgctgcagt ttttccgctc
    16321 cccgtctggg ccatacccag aacgtcgcgg ccattcagca gatgtggaat acactctgcc
    16381 tgaattggag atggtttttc gtaacccaga tcgttaaggg cttcaaggat aggagccttc
    16441 aggcccagat ctgcaaaagt ggtttcgaat tcagccatgt agtacgtgtg cctcaaaatt
    16501 aatggcggcc agtctacata actcatcatg aaattgatca gcaattttca ttgaaaagtg
    16561 tgaaccggct caaagtaggt gtattaacga acaacaacgc cctcacccgt taaggtgatg
    16621 gcaatcaaaa aagattacgg gctgatgtgt acgtcagcta ttgctggtcc gattctgcca
    16681 ggtcatcttg gtcctggccc aggagcgata attccaacaa tgcgtatcgg tgctcaacaa
    16741 agttatgaac gttgttggca accgccagtt tgaacagtgc cgtggcgctg tccaaatccc
    16801 ccagacttag gtagtactta cctaaataga agttggtttc actgagatgc tcagcgagcg
    16861 aggtgttatc cgttgcgtcc gccttgagcc tttccattaa cgtttgttcg ctaatgttgc
    16921 ccaggtagaa ctcgacaatg ttccatcccc actgttcctt atccgatttt tcgaagtgct
    16981 gtttcaacac ttctttagcc tgcttctcat cgagcttctg ctcggcgaga taaagccaca
    17041 gactacggaa aggatcattg ggatcgtctt gataaaacgc cagcagatca tcttgcgcta
    17101 acttgtcacg accgccgtaa tataatgcga tcccgcgatt caagtgcgcg tagttgtaag
    17161 ttggatcaag ctcaagtaca gaatcaaacg cttcataggc agcatcaaaa ttgcctgcct
    17221 gcgttaaata tatgcctaag taattgaata cttcaggcat atccggtcgg attgccagcg
    17281 cttgcgaaaa atcgttacgc gctaatgccc tcagaccgag actatcatac aacactccgc
    17341 gctcatataa aagctgtgcg cgttcgtcat cggttaaagc ccgactggca aggatttgtt
    17401 ccatacgtgc cagaatcact tcctgctgta aagtcggttg caatggtacc gcgaggactt
    17461 cacttttacg ccaggaagta ttactgcatc ctgcaagcgt aagtgctgtc gcaacgaaac
    17521 accagcgcaa aaaaggcttc atttcccact cccgaagacc acggttgaat gaacgtcctg
    17581 ttcccggttg ctaacaaggc gtcctgcccg gttaaaagcc ccccgccgca gcggagggca
    17641 aatggcaacc ttactcgccc tgttcagcag ccggagcttc cggtgctgca gcaggttgag
    17701 actgctcagt cgcttcttta atgctcagac ggatacggcc ctggcgatca acttctagaa
    17761 ctttcaccgg tacttcctga cccatctgca ggtaatcggt cactttctca acgcgtttgt
    17821 cagcgatttg agagatgtgg accagacctt ctttaccgcc gccgatggca acaaatgcgc
    17881 caaagtcaac gatacgggtc actttaccag tgtagacgcg gcccacttcg atttctgcag
    17941 tgatctcttc gatacgacga atagcatgtt tcgctttctc gccgtcggtc gctgcgatct
    18001 tcacagtacc gtcatcttcg atttcgatgg tagtgccagt ttcttcggtc agggcacgga
    18061 taacagagcc gcctttaccg ataacgtctt tgatcttatc cgggttgatc ttgatggtat
    18121 ggatacgcgg tgcgaactca gagatatcgc cacgcggcgc gttgatcgcc tgttccatta
    18181 cgcccaggat atgcagacgc gcacctttag cctggttcag cgcaacctgc atgatctctt
    18241 tggtgatacc ttcaattttg atatccatct gcagtgcaga gataccgtcg cgggaacctg
    18301 caactttgaa gtccatatcg cccaggtgat cttcgtcgcc caaaatgtca gacagtacaa
    18361 cgtagttgtc gccttctttc accagaccca ttgcgatacc cgcaacggca gctttgattg
    18421 gcacacctgc gtccatcagc gccagagacg cgccgcacac ggaagccata gaagaagaac
    18481 cgttggattc ggtgatttca gacactacac gtacggtgta cgggaatttg tccatatccg
    18541 gcatgactgc cagcacgccg cgcttcgcca gacgaccgtg accaatttca cgacgcttcg
    18601 gagaaccgac catgccggtt tcgcctacgg agtacggagg gaagttgtag tggaacagga
    18661 aggtatcggt acgttcgccc atcagttcat caagaacctg cgcgtcacga gcggtaccca
    18721 gcgttgcagt aaccagtgcc tgcgtttcac cgcgggtgaa cagcgcagaa ccgtgagtac
    18781 gcggcagcac gccagtacgc acatccagac cacggatcat atctttttca cgaccgtcga
    18841 tacgcggttc gcctgccagt acgcggctac gaacaacgtt tttctcgata gcgtgcagaa
    18901 tttcacccag ttcgttttcg tccagggttt cgtcttcagc aagcagcgtc gcgatggttt
    18961 cagatttgat gacatcaacc tgcgcataac gctcttgttt gtcggtgatg cggtaagcat
    19021 cactcagacg agcttcagcc agtgcagcaa cgcgcgcgtt cagcgcttcg tttaccggct
    19081 ccggctgcca gtcccaacgc ggtttaccgg cttctttcac tagttcattg atgttctgaa
    19141 taacaacctg ctgttgttca tgaccgaaca ctactgcgcc cagcatctga tcttcgctca
    19201 gcagttcagc ttcagattca accatcagta cagcggcttc agtaccggca acaaccagat
    19261 ccagtttgct ctctttcagc tcgtcctgag tcgggttcag tacgtactgg tcattgatgt
    19321 aacctacgcg ggcagcacca atcgggccat tgaacggaat accagacaga gacagtgctg
    19381 cggaagcacc aatcatcgcg acgatatccg ggttaacctg cgggttaaca gaaaccacgg
    19441 tggcgataac ctgaacttcg ttgacgaagc cttccgggaa cagcgggcga atcgggcggt
    19501 caatcagacg cgcgatcagg gtttcgcctt cgcttggacg accttcacga cggaagaagc
    19561 tacccgggat acgaccagca gcgtaggtac gctcctgata gttaacggtc agtgggaaga
    19621 agtcctgacc tggtttggct tttttctgtc caacaacggt aacgaatacc gcggtgtcat
    19681 ccatgctaac cataacagcg gcagtagcct gacgagccat catgccggtt tccagagtca
    19741 cggtgtgttg gccgtactgg aatttacgaa cgatcggatt aagcaatgta atatcctttc
    19801 tctttcttag acagtacctt acggcactgg tgttaatacc cgatcttctg cgcatcctcg
    19861 cgactaatga caaccctaac ccagctctat gtgggtaaag cctctcatta gccgcgcgaa
    19921 cctctgcaac ggaagatcat tcatagcaac aatacattag tttccagtga attgctgccg
    19981 tcagcttgaa aaaaggggcc actcaggccc ccttttctga aactcgcaag aattagcgac
    20041 gcagacccag gcgctcgatg agctgggtgt aacgtgctac gtctttacgt ttcaggtagt
    20101 cgagcagttt acgacgctga gaaaccatgc gcagcagacc acgacggctg tggtgatctt
    20161 ttttgtgctc tgcaaagtgg ccctgcaggt ggttgatctg tgcagtcagc agtgctacct
    20221 gaacttcggt agaaccggtg tcgtttgcgt cacgaccaaa ctcagaaacg attttagctg
    20281 ttgcttcagt acttagagac attttaaaac tccaaagtat atagaatgaa aggacgccga
    20341 tctctaattc agcgatccca gtgtacgtta cgcaaagtgt taaacaattt acgcgacgtt
    20401 aagcggcagt attctactcg tagcgacctg ttatcgcaag acggttaaca ttacgccggg
    20461 tattcaacca ccaggcgacg aggcgcgacg cggccttcat cgtcaatttc gcccataccg
    20521 ataaacttgc cgttctcacc ttctgtaacg cgaaccagtc cttccagcgg cgcaccggat
    20581 gtacgaactg ggttaccatt tttgaagtaa acagaagacg ttaacggaag attcaccacc
    20641 gggtagtccg aagctggact gtccattggc atcagtaatg gatcaagtaa ctccgcagcc
    20701 ggaatatcct gctgttcggc ttgttcgacc agttcacgca gatgttccag ggtcaccatc
    20761 cgttcaaccg gatatttact taccgccaga cgacgcaggt aaataacatg cgcgccacaa
    20821 ccgagttttt cacccaggtc atcaatgatg gtgcggatat aagtgccttt tgagcagtga
    20881 atttccagct ccagctcatt gccttcgtgg cgaataaaca gcaattcata aacggtaatc
    20941 gggcgcgctt cacgcggtac ttcaatgccc tgacgcgcat attcgtacag ttttttaccc
    21001 tgatatttca gtgctgaata catcgaaggg atctgttcga tatcgccacg gaaagtatcc
    21061 agcgccgccg ccagttgctc tgcgctaaag gttaccggac gttcttcaac gatttgtccg
    21121 tcggcatcag aagtatcggt acgctgtcca agacgcgcaa tgacccgata gcgtttgtcg
    21181 gagtccagca gatactggga aaacttcgtc gcttccccga ggcaaattgg caacatgcct
    21241 gttgccagcg ggtccagcgc accggtatgc ccggcacggt tggcgttata aatccgtttc
    21301 actttttgca gcgcatcatt gctggacata ccctgcggtt tatccagcaa caaaacgccg
    21361 ttaatgtcgc gaccgcgacg acgaggacga ctcattagtc ctccttgctg tcgtccgggt
    21421 taacacgacg ttcttcgtca tgtttgacca cgctggtcac caggtttgac atgcgcatcc
    21481 cttcaaccag agagttgtcg tagaagaagg tcagttccgg cacgatacgc aggcgcatcg
    21541 ctttccccag caggctgcgg atgaaaccag aagcttcttg caacgctttg atgcccgctt
    21601 taaccgcgtc ttcatctttg tcgttgagga acgtcacata tactttggca tacgccaggt
    21661 cgcgagacat ttcgacaccg gaaacggtgg tcatcatgcc caggcgagga tctttaattt
    21721 cacgctgcag gatgagagcg atctcttttt gcatttcctg cgctacgcgc tgcgggcgac
    21781 caaattcttt cgccataata aattctcctg acaaaaaagg ggctgttagc ccctttttaa
    21841 aattaatttc aggtggaagg gctgttcacg ttggcctgat aagacgcgcc agcgtcacat
    21901 caggcaatcc atgccggatg cggcgtaaac gccttatccc gcatggaacc ctaaaaacct
    21961 taagcgatgg tacgttggat ctcaatgatt tcgaatactt cgatcacatc gccagtgcgg
    22021 acgtcgttgt agttcttaac gccgatacca cattccatac cgttacggac ttcgttaacg
    22081 tcatctttga agcggcgcag ggactccagc tcgccttcgt agataaccac gttgtcacgc
    22141 agaacgcgga tcgggttgtg acgtttaacc acaccttcgg taaccataca gcctgcgatg
    22201 gcaccaaatt tcggcgattt gaacacgtca cgaacttccg ccagaccgat aatctgctgt
    22261 ttcagttccg gagacagcat accgctcatc gccgctttca cttcgtcaat caggttatag
    22321 atgacggagt agtaacgcag atccaagctt tccgcttcaa tcactttacg tgcagaggca
    22381 tcagcacgta cgttgaagcc aaccaggatg gcgttggacg ccgcagccag ggtggcgtcg
    22441 gtttcggtga taccacctac gccagaaccg atgatcttca ctttaacttc gtcagtagac
    22501 agtttcagca aggagtcgga gatcgcttcc acagaaccct gtacgtctgc cttcaggacg
    22561 atgttcactt cgtgaacttc gccttcggtc atgttggcga acatgttctc gagtttagat
    22621 ttctgctgac gcgccagttt aacttcgcgg aatttaccct gacgatacag tgcaacttca
    22681 cgcgctttct tctcgtcacg tacaacggta acttcatcac ccgcagccgg tacgccggac
    22741 aggccgagga tttccaccgg aatggacgga cccgcttcca gcacttcctg acccagttcg
    22801 ttacgcatcg cacgaacacg accgtattcg aagccacaca gaacgatatc gcccttgtgc
    22861 agagtacctt cacgtaccag aacggtagca accggaccac gacctttatc gaggaaggat
    22921 tcgataaccg caccgctcgc cataccttta cgtaccgctt tcagctccag aacttccgcc
    22981 tgcagcagga tagcgtccag cagttcatcg atgccggtac ccgctttcgc agatacgtgt
    23041 acgaactggc tttcaccgcc ccactcttcc ggcaggatgc catactggga gagttcgttc
    23101 ttaacacgat ccggatcggc ttctggttta tcaatcttgt tcactgcaac caccaccggt
    23161 acctgcgccg ctttcgcgtg ctggattgct tcgatggtct gcggcatcac gccgtcgtcg
    23221 gcagcaacaa ccaggactac gatgtccgtt gcctgcgcac cacgtgcacg cattgaagta
    23281 aacgcggcgt gccccggggt gtccaggaag gtgatcatgc cgttttcagt ttcaacgtgg
    23341 tatgcaccaa tgtgctgggt aatgccgccc gcttcgccag aggcaacttt cgttgaacga
    23401 atgtagtcca gcagagaggt tttaccgtgg tcaacgtgac ccatgatggt cacaaccggc
    23461 gcgcgcggtt cagccgcagc acccgtgtca cggtcgctca ttaccgcctc ttccagctcg
    23521 ttttcacgac gcaggataac tttgtggccc atctcttcag caaccagctg tgcggtttcc
    23581 tgatcgataa cctggttgat ggttgccatt gcgcccagtt tcatcatcgc tttgatgacc
    23641 tgagagcctt taaccgccat cttgttcgcc agttcgccaa cggtgatggt ttcgccgatc
    23701 acaacgtcac ggttaacggc ctgagctggc ttctggaagc cttgctgcag ggaagaacct
    23761 ttacgttttc cgcctttacc gccacgtact gctgcgcgtg cttcttcacg atcagctttt
    23821 gattcagcgt gtttgttgcc tttcttcgga cgcgctgctt tcgcgttacg accacggcca
    23881 cgaccgcctt cgacttcacg atcgctttcg tcttctgcct ggcgagcatg ttgagaagta
    23941 gtgacgtgat aatcgctgga atcttcagtc ggttccgcgt tatcagtcca tttgttttct
    24001 tccgccatac gacgtgcttc ttcagcaacg cgacgtgctt cttcttcgag tttacgatgc
    24061 gcttcttctt cggctttacg cttgagctct gcagcttcct gctcacggcg ggctttttca
    24121 gcctgggcgt ttttagtcat atcgtcttgt tgattgctca ctttgtcttt ttccgcagct
    24181 tcacgtttcg cttgttcagc agcttcacgc ttagcttgtt ctgcagcctc acgttcagct
    24241 ttttgttgcg cctcgcgttt agccgattct tctgcctcac gacgggcttg ctcttccgct
    24301 tcacgctgcg cttgctcttc cgctgcaagg cgttcagcct cttgcggatc gcgtttcaca
    24361 aaggtgcgtt tcttgcggac ttcgatttgt accgatttgc tttttccacc ggtaccagga
    24421 atgttaaggg tgctgcgtgt tttacgttgc agcgtcaatt tgtccgggcc tgaatttttc
    24481 tgattcaggt ggtcaatcaa agtctgtttc tcttgtgcag acacagagtc gtcagcagac
    24541 ttccggatac ctgcatcagc aaattgctgt accaggcgtt ccacggaggt ctgtcgctct
    24601 gcggccagcg ttttaatcgt tacatctgtc atgctgttcc ttcctgctac agtttattac
    24661 gcttcgtcac cgaaccagca aatattacgg gcagccataa tcagtgctcc ggccttttcg
    24721 tcggtcaacc cttcgatatc agccagatca tcaatgccct gttcggcgag atcttccagc
    24781 gtacaaacgc cacgggcggc cagtttgaat gccaaatcac gatctacccc ttcaaggttc
    24841 agcagatcgt cagccggttt gttatcaccg aggctttctt cctgggcctg tgcaatggtg
    24901 gccagtgcat ttttagcacg ctcgcgcagt gcttcaacgg tcggctcatc aaggccttcg
    24961 atttccaaca gctctttcat cggcacatag gccagttctt ccagcgtcga gaagccttct
    25021 tctaccagaa cagtcgcgaa gtcttcgtcg atgtcgagat atttggtgaa ggtgtcgatc
    25081 gctgcgtgcg cttccgcctg atgcttagcc tgcaggtcgt caacggtcat cacgttgagt
    25141 tcccaaccgc tcagctgcga agccagacgc acgttctgac cgttacggcc aatcgcctgc
    25201 gccaggttac cggcttcaac ggcgatatcc atggtgtgtt tatcttcatc caccacgata
    25261 gaagcaacgt ctgccggtgc cattgcgtta atcacgaact gcgccgggtt atcatcccac
    25321 aggacgatat cgatacgctc gccacccagt tcagtagaca ccgcctgaac acgcgcgcca
    25381 cgcataccta cgcaagcacc taccggatcg atacgtttat cgttggtttt caccgcgatt
    25441 ttcgcacgag aacccggatc gcgagccgct gctttaattt caatcacttc ttcgccgatt
    25501 tctggcactt caatacggaa cagttcgatc agcatttccg gcttggaacg agtgacgaac
    25561 agttgcgcgc cacgcgcttc cgggcgaacg gaataaagca cgccacgaac gcggtcgcca
    25621 gggcggaagt tttcacgcgg cagcatatct tcacgcagga tcacggcttc agcgttgttg
    25681 cccagatcca gagagatgtt gtcgcggttt acttttttca ccacgccggt gatgatttca
    25741 ccttcgtgtt cacggaactg atcaaccacc atcgcacgtt cggcttcacg cactttctgc
    25801 acgataacct gttttgccgt ctgggtagtg atacggtcaa aggtaacaga ctcaatctga
    25861 tcttcaacgt aatcgcccag gttcaggctt tcatcttcat aacgtgcggc ttcaagggtg
    25921 atttccttgg tcggctgggt gacttcatca acaactaacc agcgacggaa ggtgtcaaaa
    25981 tcaccgcttt tgcgatcgat ctgtacgcgg acgtcgatct cttgttcata ttttttcttt
    26041 gttgctgtcg ccagcgcgct ttccaatgct tcgaaaatct tctcgcgagg tagcgccttt
    26101 tcattggata cggcttcaac tacagccaaa atttctttgt tcatcgcggg cttttcacct
    26161 catccagact attaaaagtg gggaaccagg ttcgccttct ggatattact cagcgcgaac
    26221 acttcatctt taccttcgac ggtaactgtg atcatttcac cgtctaccgc tttgataacg
    26281 ccctgccatt tacgacggtt ttgtaccgcc atacggagaa ccagagtcac ctcttctccg
    26341 acaaaacggg cgtagtgttc agccgtgaac agtgggcgat cgagacccgg tgaggagact
    26401 tccaggttat aagcaacggt gatgggatct tcaacatcca gcacagcact tacctggtgg
    26461 ctcacatcag cacaatcatc aacattgatg ccatcttcac tatcaatata gatgcgcagt
    26521 gtggatgtgc gaccgcgaat aaattcgatg ccaaccagtt caaaacccag ggcctcaact
    26581 ggcgcagtaa tcatctctgt taatttttgc tctaatgtgg acaagcccac ccccaagaca
    26641 taaaaaaagg gcctaaagcc cagttattct gtagtcagat aacaaaaaac cccgataaat
    26701 cggggcttta tataactgaa ccctataacc gcaactgcgg tctggagcac tttccagaag
    26761 gattttttca aatcccacta cgaaggccga agtcttcaca gtatatttga aaaaggactc
    26821 taagggaaag tggttgcggg ggccggattt gaaccgacga ccttcgggtt atgagcccga
    26881 cgagctacca ggctgctcca ccccgcgcct gaaacgtggc aaattctact cgttttgggt
    26941 aaaaaatgca aatactgctg ggatttggtg taccgagacg ggacgtaaaa tctgcaggca
    27001 ttatagtgat ccacgccaca ttttgtcaac gtttattgct aatcatgtga atgaatatcc
    27061 agttcacttt catttgttga atacttttgc cttctcctgc tcccccttaa
//
LOCUS       DAESHI010000050        25774 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.050, whole genome shotgun sequence.
ACCESSION   DAESHI010000050 DAESHI010000000
VERSION     DAESHI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25774
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.050"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..827)
                     /gene="dgt"
                     /locus_tag="KW011_002196"
     CDS             complement(<1..827)
                     /gene="dgt"
                     /locus_tag="KW011_002196"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HBH7660519.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVA"
     gene            911..1609
                     /gene="mtnN"
                     /locus_tag="KW011_002197"
     CDS             911..1609
                     /gene="mtnN"
                     /locus_tag="KW011_002197"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBH7660520.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            1602..2402
                     /gene="btuF"
                     /locus_tag="KW011_002198"
     CDS             1602..2402
                     /gene="btuF"
                     /locus_tag="KW011_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HBH7660521.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            2440..3063
                     /locus_tag="KW011_002199"
     CDS             2440..3063
                     /locus_tag="KW011_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HBH7660522.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(3110..3454)
                     /gene="erpA"
                     /locus_tag="KW011_002200"
     CDS             complement(3110..3454)
                     /gene="erpA"
                     /locus_tag="KW011_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HBH7660523.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(3447..3512)
                     /locus_tag="KW011_002201"
     CDS             complement(3447..3512)
                     /locus_tag="KW011_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660524.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            complement(3536..4957)
                     /gene="clcA"
                     /locus_tag="KW011_002202"
     CDS             complement(3536..4957)
                     /gene="clcA"
                     /locus_tag="KW011_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HBH7660525.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            5182..6462
                     /gene="hemL"
                     /locus_tag="KW011_002203"
     CDS             5182..6462
                     /gene="hemL"
                     /locus_tag="KW011_002203"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HBH7660526.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            complement(6620..8602)
                     /gene="fhuB"
                     /locus_tag="KW011_002204"
     CDS             complement(6620..8602)
                     /gene="fhuB"
                     /locus_tag="KW011_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HBH7660527.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8599..9489)
                     /gene="fhuD"
                     /locus_tag="KW011_002205"
     CDS             complement(8599..9489)
                     /gene="fhuD"
                     /locus_tag="KW011_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HBH7660528.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            complement(9489..10286)
                     /gene="fhuC"
                     /locus_tag="KW011_002206"
     CDS             complement(9489..10286)
                     /gene="fhuC"
                     /locus_tag="KW011_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HBH7660529.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            complement(10337..12526)
                     /gene="fhuA"
                     /locus_tag="KW011_002207"
     CDS             complement(10337..12526)
                     /gene="fhuA"
                     /locus_tag="KW011_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HBH7660530.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            complement(12746..15280)
                     /gene="mrcB"
                     /locus_tag="KW011_002208"
     CDS             complement(12746..15280)
                     /gene="mrcB"
                     /locus_tag="KW011_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HBH7660531.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            complement(15476..17905)
                     /gene="hrpB"
                     /locus_tag="KW011_002209"
     CDS             complement(15476..17905)
                     /gene="hrpB"
                     /locus_tag="KW011_002209"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HBH7660532.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            17979..18509
                     /gene="thpR"
                     /locus_tag="KW011_002210"
     CDS             17979..18509
                     /gene="thpR"
                     /locus_tag="KW011_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HBH7660533.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            18524..19228
                     /gene="sfsA"
                     /locus_tag="KW011_002211"
     CDS             18524..19228
                     /gene="sfsA"
                     /locus_tag="KW011_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBH7660534.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            19406..19861
                     /gene="dksA"
                     /locus_tag="KW011_002212"
     CDS             19406..19861
                     /gene="dksA"
                     /locus_tag="KW011_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HBH7660535.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            19898..20824
                     /gene="gluQRS"
                     /locus_tag="KW011_002213"
     CDS             19898..20824
                     /gene="gluQRS"
                     /locus_tag="KW011_002213"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HBH7660536.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            20863..22281
                     /gene="pcnB"
                     /locus_tag="KW011_002214"
     CDS             20863..22281
                     /gene="pcnB"
                     /locus_tag="KW011_002214"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HBH7660537.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            22278..22757
                     /gene="folK"
                     /locus_tag="KW011_002215"
     CDS             22278..22757
                     /gene="folK"
                     /locus_tag="KW011_002215"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBH7660538.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            23127..23711
                     /locus_tag="KW011_002216"
     CDS             23127..23711
                     /locus_tag="KW011_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBH7660539.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            23816..24556
                     /locus_tag="KW011_002217"
     CDS             23816..24556
                     /locus_tag="KW011_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBH7660540.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            24591..25774
                     /gene="htrE"
                     /locus_tag="KW011_002218"
                     /pseudo
     CDS             24591..25774
                     /gene="htrE"
                     /locus_tag="KW011_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT         6330 a         6986 c         6416 g         6042 t
ORIGIN      
        1 tctgccacac aataggagat gtcgtcggca gcttccataa tccacgttaa tggaaaacga
       61 ctgtaaagcg ccaaattaag ttctttacgc aaccgggcaa tataggcttc ttcagaaaga
      121 taataacccg gctttttcat taaatagtga tgtgtctcag gcgtttcgcc acgccaccac
      181 gccggacggg tatattttaa aataccgcca acctgtgccc aagtgagatt catccgcatc
      241 aatgtatgca ccagacgaat gccttgtgca ttcccctcaa aatgacataa gtcctgacga
      301 atcttgcgcc gcagctcgtt aagctgttct tccccgtccc gtaaacgtag tgccgccacg
      361 ctgcagcgat cgtcagtcag aggctggctt tcggcatctt ccgggtgcaa acgttggcga
      421 aaccagtcat ttatcgctgc ttcgccaaaa tgaccaaacg gcggattgcc gatatcgtgc
      481 atcaggcatg acatctcaac aatgctttca aaggggccgg tcagttcatc caggccgtat
      541 gcttccagca atttaagctc tttcagacgg cttaaaattt ctttggcgat gtagcgtccc
      601 acctgctgga cttccatcga gtgggtaaga cgcgtgcgca cggcggcatt gcgctccagt
      661 ggaaaaacct gggtcttttg ttgcagacga cgaattgccg gagagttgat gatacgcccg
      721 cgatcgctct cgaagatccg caggatctca tgttcggttt taacgccctg cggtgaacgg
      781 taacgacgat gccagtttat tttttttcgg aaatcaatct gtgccatgtc ctctcccgcg
      841 tgagaaatac gcttccccgt aagcgcatgg taaactatgc tttcaaatcg ggcttatcgc
      901 gagtaaatct atgaaaatcg gcatcattgg tgcaatggaa gaagaagtta cgctgctgcg
      961 tgacaaaatc gaaaaccgtc aaactatcag tctcggcggt tgcgaaatct ataccggcca
     1021 actgaatgga accgaggttg cgcttctgaa atcgggcatc ggtaaagtcg ctgcggcgct
     1081 gggtgccact ctgctgttgg aacactgcaa gccagatgtg attattaaca ccggttctgc
     1141 cggtggcctg gcaccaacgc tgaaagtggg cgatatcgtt gtctcggacg aagcgcgtta
     1201 tcacgacgcg gatgtcacgg catttggtta tgaatacggt cagttaccag gctgtccggc
     1261 aggctttaaa gctgacgata aactgatcgc tgccgctgag gcctgcattg ccgaactgaa
     1321 tcttaacgct gtacgtggcc tgattgttag cggcgacgct ttcatcaacg gttctgttgg
     1381 tctggcgaaa atccgccaca acttcccaca ggccattgct gtagagatgg aagcgacggc
     1441 aatcgcccat gtctgccaca atttcaacgt cccgtttgtt gtcgtacgcg ccatctccga
     1501 cgtggccgat caacagtctc atcttagctt cgatgagttc ctggctgttg ccgctaaaca
     1561 gtccagcctg atggttgagt cactggtgca gaaactggca catggctaag tcactgttca
     1621 gggcgctggt cgccctgtct tttcttgcgc cactgtggct caacgccgcg ccgcgcgtca
     1681 tcacgctttc tcccgccaac actgaacttg cctttgccgc cgggatcacg ccggttgggg
     1741 tcagcagcta ttccgactat cctccacaag cgcaaaagat tgagcaggtt tccacctggc
     1801 aggggatgaa tctggaacgc attgtcgcgc tgaaacccga tctggtgatt gcctggcgtg
     1861 gaggtaatgc cgagcggcag gttgaccagc tggcttcgct gggaataaaa gtgatgtggg
     1921 tcgatgcgac aagcattgaa caaattgcca atgcgttacg tcaattggcc ccctggagtc
     1981 cgcaaccgga taaggccgaa caagccgcgc aatccctgct ggatcagtac gcgcaattga
     2041 aagcgcaata tgctgataaa cctaaaaaac gtgtttttct gcaattcggc attaatccgc
     2101 catttaccag tggaaaagag tcgattcaaa accaggtact cgaagtttgt ggcggagaaa
     2161 acatctttaa agacagccgg gttccctggc cgcaagttag ccgcgaacag gtgttagcac
     2221 gctcgccaca ggcgattgtc attacaggcg gaccggacca aattcctaaa atcaaacaat
     2281 actggggtga acagctcaaa attcccgtta ttcctctcac gagtgactgg tttgaacgtg
     2341 caagcccacg tattatcctc gctgcacaac agctctgtaa tgcgctttca caggtagatt
     2401 agcgcctgac ctttttaggt gctcagtggg gattgaacaa tgctcgtcta ttggctggat
     2461 atagtcggca cagcggtatt tgccatctcc ggcgttttgt tagccggaaa attgcgtatg
     2521 gacccttttg gtgttctggt actgggcgtg gttaccgctg taggcggcgg gacaattcgc
     2581 gacatggcgc tggatcacgg cccggtattt tgggtgaaag atcccaccga tctggtcgtt
     2641 gcaatggtaa ccagcatgct gaccatcgtg ctggtgcgcc agccaagacg cttaccaaaa
     2701 tggatgttgc cggtgctgga cgccgttggt ctggcggtgt ttgtcggcat tggcgtgaat
     2761 aaagccttta atgcggaagt cggtccgtta atcgcggttt gtatggtcgt cattactggc
     2821 gttggcggcg ggatcattcg tgatgtactg gcccgcgaga tccccatgat tttacgtaca
     2881 gaaatctacg caactgcctg tattatcggc ggtattgtcc acgctacggc ttattacaca
     2941 ttttccgtac cactggaaac agccagtatg atgggcatgg tcgtgacgct attgattcgg
     3001 ctggcggcta ttcgttggca tcttaagcta ccgacgtttg cgctggatga gaatgggcgt
     3061 tgaagagaga agatgcgccg gatagtttat ccggcacatg aacaacagat tagatgctaa
     3121 aggaagaacc gcaaccgcag gtgcttttcg cgttcgggtt ggtcacgatg aaacgagaac
     3181 cttccagtcc ttcggtatag tcaacggaac cgccgaccag atattgcagg ctcatcggat
     3241 caaccaccaa gccaacgccc tgtttttcga tggtcatatc gccttcgttc acctgatcat
     3301 caaaggtgaa accatactgg aagccgctgc aaccgccacc tgtgatatac acgcgtaatt
     3361 tcagattcgg gttatcttcg tcagcgatca ggcttttaac tttgttggct gctgcgtcgg
     3421 taaactccag cggcagtgct acgtcatcac tcatattttg ctccaaacga catcggcaat
     3481 tgggcaaatt ctaacccaat aataatcgcc attatctaat accctggtat ttcgttcaag
     3541 tattctcgct ggctgatgcg gccttgcttc gcgccagttg ctcagcttcc tgttttgcca
     3601 gcgtgcgcgc aagaatcgcc gagtatagcg gtttcccgcc ggtaaattgc gctaatagtg
     3661 ttgcgccaag accggtaata atcattggca aaatgagctg gtagttatcg gtcatctcca
     3721 gaaccagaat gatccccgtt aacggcgcgc gaatagatgc cgccagtaat gcccccattc
     3781 cggcaatagc aaacgtcccc gcctcaaggt gatattgcgg aaacagctca acggcaacca
     3841 ttccgaaagc ggttcccagc acagtaccca gcgccagcat cggggcaaaa ataccgcccg
     3901 gcgcgccgga agagaagcag agtaaggtgg taatgacccg agcgacgaag ataaacacca
     3961 gcattcccat gctgaaattc cccgcggtag cgataggaat caggttaaaa ccgccgcccg
     4021 acgttgctgg tgccacaaac cccagcaatc cacacagacc gccaatcgca ccgcccatca
     4081 gtacccattt ggtaatattg ccgccgtgca cacggtgcag caaatcctgc atccccagca
     4141 cccatttatt aaaaataggg ccgaaaatgc caaaaataat accgaggatc agataaagcc
     4201 acagcgtatt aagcggcgca tcagaaagtt taccgacgtc aatcaacgca acttcatgat
     4261 taaaaatccg gtacataatg gtcgacataa tgacgccaat aaataccgct tttatcgaaa
     4321 ttaacgtata gcgaaactgc ggacgcatct cttcgataat aaacaaaata cccgccagcg
     4381 gcgcgttaaa ggccgcagcc agccccgcag cagcaccggt tgccagcagc gtatggcgag
     4441 cttcgtcacc tttcaggcgg aaaatatcaa gcaccatacg gccaatgtta ccgccgatct
     4501 gcacggttgg cccttcgcgc cccaacacca tgcctccgcc gagtgtcccc agcccgccaa
     4561 agaacttcac cggcaataca cgccaccagc gaacgggacg ttgatcttcc agcgcccctt
     4621 caatttccgg gatccccgaa ccacctgctt ccggcgcgta tttgcgcacc aaaaagtagc
     4681 caaacatcgc cagcaccgcc gaacagagaa aagcgacggt taacagaagc ggataattat
     4741 cagcagtatg taccagcgcc cccatacgtt ggttctgcaa ccaggcgaca cctttgtcaa
     4801 aagcaaccgc tgccagccca acaagcgtgc cgacgactgc cgccataaac aaaatggcta
     4861 acggggtttt atcgcgctca agaagttggc gaatcagttg tctgcgtcgc aggcgcgcgg
     4921 cctgcggtgt ttctaaagag ggagtatcag ttttcatcca atgatcactt attggtcata
     4981 caaataagat gacggcattt tactcgcacg ttccatgaaa atcctttgca aagcgtaatg
     5041 tttcaaatgc acaaaacttt tatactctaa gaaatcagga ttgcaccagt acaagcagcc
     5101 tgatgtttga cgagtattta acttgttatg aataacatag aatagcagcc attcactttt
     5161 tctacgaatc aggaaccctc catgagtaag tctgaaaatc tttacagcgc agcgcgcgag
     5221 ctgatccctg gcggtgtgaa ctcccctgtt cgcgccttta ctggcgtggg cggcactcca
     5281 ctgtttatcg aaaaagcgga cggcgcttat ctgtacgatg ttgatggcaa agcctatatc
     5341 gattatgtcg gttcctgggg gccgatggtg ctgggccata accatccggc aatccgcaat
     5401 gccgtgattg aagccgccga gcgtggttta agctttggtg caccaaccga aatggaagtg
     5461 aaaatggcgc aactggtgac cgaattggtc ccgaccatgg atatggtgcg catggtgaac
     5521 tccggcaccg aggcgacgat gagcgccatc cgtctggccc gtggttttac cggtcgcgac
     5581 aaaatcatta aatttgaagg gtgttaccac ggtcacgctg actgcctgct ggtgaaagcc
     5641 ggttctggcg cactcacgtt aggccagcca aactcgccgg gcgttccggc agatttcgcc
     5701 aaacatacct taacctgtac ttataacgat ctggcttctg tacgcgccgc gtttgagcaa
     5761 tacccgcaag agattgcctg tattatcatc gagccggtgg caggcaatat gaactgtgtt
     5821 ccaccgctgc cagacttcct gccaggtctg cgtgcgctgt gcgacgaatt tggcgcattg
     5881 ctgatcatcg atgaagtaat gaccggcttc cgcgtggcac tggctggcgc acaggattat
     5941 tacggtgtgg aaccggatct cacctgcctg ggcaaaatca tcggcggtgg aatgccggta
     6001 ggcgcattcg gtggtcgtcg tgatgtaatg gatgcgctgg ccccgacggg tccggtctat
     6061 caggcgggta cgctttccgg taacccaatt gcgatggcag ccggtttcgc ctgtctgaat
     6121 gaagtcgcgc aaccgggcgt tcacgaaacg ttggatgagc tgacaacacg tctggcagaa
     6181 ggtctgctgg aagcggcaga agaagccgga attccgctgg tcgttaacca cgttggcggc
     6241 atgttcggta ttttctttac cgacgccgag tccgtgacgt gctatcagga tgtgatggcc
     6301 tgtgacgtgg aacgctttaa gcgtttcttc catatgatgc tggacgaagg tgtttacctg
     6361 gcaccgtcag cgtttgaagc gggctttatg tccgtggcgc acagcatgga agatatcaat
     6421 aacaccatcg atgctgcacg tcgggtgttt gcgaagttgt gatccagcat ggtttgtcgg
     6481 atgcggcgtg aacgccttat ccgacctacg attcggggcc gtttgtaggc ccgataagac
     6541 gcgccagcgt cgcatcgggc atccgttgtc gcgtaaacgc tttatccgtc caggcgtaca
     6601 gggccgttat atggaaaaat taacggctct gctttctcag caaatagata aaatatggcg
     6661 cgccgataaa ggtcgacagc agccccgccg ggatctggaa tggaaacagc accatccgcc
     6721 cacaccagtc agcgaatacc agcagtaaac cacccaccag cgccgaaatt acgatgtgtg
     6781 gcatcgtccg tcgaaagccc atcatccgcg caatatgcgg tgccattaaa ccaacaaaac
     6841 tcaacggtcc aatagtcatc gtcgcggtcg ccgtcaggca agccgctaac agcagcagcg
     6901 caattcgcgt cggcgtcagc gccattccta cggctcgtgc ggtatcacca cccagcggta
     6961 aaatggtcag ccagcggcgg tacagcgggg taatcgccag caaaatcacc atcacaattc
     7021 cggtacgcca gacctgcgca tcggtcgcgt tgtaggtcga accggaaatc caggtcagca
     7081 cttgcgccat tcgcgggtca ccgctcgcct gcaacatcat caaaagcatg gtgaatgcgg
     7141 tacttaacgc catccccgcc agtaacatac ggtgtgggga aaatccaccg cggccggcgg
     7201 cgatcataat gatcaacagc gtcaccgccg ccccgatact gcccgcgggt aacagccagc
     7261 caaaggcatt acccggcacc agaaacagca ttaacaccac gccaaacgcc gcgccagagc
     7321 taatccccag cacttccggg cttgccatcg ggtttccggt cagccgctga ataatacagc
     7381 ccgccaccgc cagcatgacg cccgcaaaca gcgccgccat aattcgcggc cagcgccagg
     7441 gcatcaaatc ctcgagcaac gccccgctcg cccacgtcca gccgtgcgca tcacgaccaa
     7501 acgacagcgc caccaccaca gccatcaaca gcagcacgcc gcccgcgagg gcaaacgcca
     7561 gcacatgttg gcgttcagcc gcgacacgat cgttgacctt catatccggc gcgctaatgc
     7621 tgcgtaaacg cggcaacagc cacagtagca gcggcgcacc gatcaacgca gtgaccgaac
     7681 cggtggacac ttccatccac acgcgagtca gccagaggat gatttgatcg gaaagccaga
     7741 ggatcagcgc cccaatcagc gacgccagca tcaaccgtgg caacaaacgc cgcgccccca
     7801 gcattttcgc cagcagcggc gcgaacaaac cgataaagcc gataatcccc accgcattca
     7861 ccagcagcgc actgatgact atcgccagcg acagcgccgc cagacgcgcg agcgacaagg
     7921 ccagcccgag attgcgcgcc acgccatcat caagccccat cagggttaac gggcgaagta
     7981 gcaataacgt caacatcacg ccgcccagca actgcggcca taaacgctca acgccgcccc
     8041 agtcggtttg cgtcagcgtt ccggtgctcc acaggaacat gctttgcagc tggtcgtgat
     8101 ggaagataac cagtagctga ttaattgccc cgcaataaag gctcaccacc agccccgcga
     8161 gaataagcgt aaccggcgaa agccgtttcc cccacgcgac gccaaagacg attaagccaa
     8221 cgacacaagc gcccaccagt gcggcaaact ggctcgccat agcacctggg atcgcccaaa
     8281 gtgtggtgac ggtaattccc agttgcgcgc ctgtagcaac gccaagcgtc gtcggctccg
     8341 ccagcgggtt acgcagcact tgctgaaaca gcacgcccac cagccccagg cccgcgccca
     8401 ccagcagcga aatcgccaga cgcggcaaca agctgtagtg aaaaatcatc tgctcgatga
     8461 catcgatgtc tggcgaccag gcagcctgcg cccactggct acgcggcagc gcctgcgaga
     8521 agttcatcca ggtgagcgcc gtagcgacaa tcactaacag cgccagcaat aacgccggaa
     8581 aaagcgcaat tcgtttactc acgctttacc tccgatggcg ttatccagaa cgcgcacaaa
     8641 gtgcattgcc gagagtgtcg caccataaaa ccagactgca ggtacgcgct gaaagcgtcc
     8701 ggcgcggaca aacggcatgg cctgccacag cggggttgcc attagcgcat ccatgtcttt
     8761 gctgttgtcg tgatcaaaac agagcacatc aacgtcttta tacgctgcca gacgatcgat
     8821 actgacggcg gtactgcccc agaagttggt ttccccttgc caggcatttg ggatgccgta
     8881 ctcatcgaga atttcctgga acaagctgtt tggaccgaag accagcatat ggcgcggatc
     8941 gataagcgtc gtcagcagta acgggcgtgc gccacgcttc acaaagcggg gtttcatgct
     9001 gcggataaag tcttcgtatt gcgctaaatg cgtttccgct gcgctttgca ggttaagtaa
     9061 atctgccatt tccgtcagcg atttacgcgc catcgccaac ggctgtttgc cgtcactgaa
     9121 gttaaatccg cgacccggcg caatacgtgc cagcatttct gatgaagggc catatcctgc
     9181 cgaccagacc ataaacgacg gtttcatttc ggtcagcagt tcaaggttag gttctgtacg
     9241 caaaccgacg tcaatcactg agtccggcaa tggtggttcg ctgacccaca ggcgatagtt
     9301 gatggtatcc gccacgccgt aaggcacgat gccgagcgcc agcagtaatt ccaccggcaa
     9361 ccactccagc gccacaatac gattggggtc aatagccgcc gcgtgggcgg tattcatctg
     9421 ccataacaac ggagaaagcg ccatcgccgt taacagtcga cggcgcgaaa taagaggtaa
     9481 gccgctcatc aataaacaaa actcacaggt gcagcacccg ccggatgcgg caaaataccc
     9541 atcgggatgc cataaatcat ttcgagggtt tcgccgcgca taatttccgc aggcgttccc
     9601 tgagcaatca tttcaccact gcgcagggcg accagataat cacagtagcg tgccgccata
     9661 ttgatatcgt gcaacacggc aatgaccgtc aggccacgtt cctgacttaa acggtgcacc
     9721 agcgccagca catcaacctg gtgggcgata tccagcgccg aagtcggttc gtcgagcaac
     9781 agacaacggc tatcctgcgc caccagcatg gcgatccacg cccgctgacg ttcgccgcca
     9841 gagagactat cgaccagccg atgcgccagc ggttttaagc caaccagcga gatagcttcc
     9901 tcgacctttt cgcgatctgc cgcaccaaag cgccccagcg cgccatgcca cgggtaacga
     9961 ccaatcgcca ccagttcacg cacggtcatc ccttctgccg gaggaagctg ctgcggcaaa
    10021 taagccactt tgcgggcaaa cgctttgctg ctccagcttt ccagcggctg ggcatcaaga
    10081 agaatctccc cttctgacgg aggctgatga cgaccaagca ttttgagcag agtggattta
    10141 ccagaaccgt tgtgaccaat cagaccggtc actttcccgg caggaaaggt taacgacagc
    10201 ggatgcaaaa gcgtgcgccc gggcacacga aaggagatat tacgcagtgc aaaagtggta
    10261 tcggaatgat tcgtgtattc ctgcataaca gccaacttgt gaaatgggca cggaaatccg
    10321 tgccccaaaa gagaaattag aaacggaagg ttgcggttgc aacgacctga cgttctgcgc
    10381 cccagaagca gccataagtc tggaagcaac tggcaacata ctcacgatcg agcaaattgt
    10441 tgacgttcac agcaaggctg gatcccgcca taccaaagcg tgccagatca tattttacaa
    10501 cagcatccat tacagctgcg ctacccactt taaaagtgtt tgccggatca ccatagctgg
    10561 aaccaataaa acgaccacct gttcccaatg ttaaaccaga aagcggccct tcattgaagg
    10621 tataatcccc ccagagagat gccatatgct ccggtacttg ttctggagtt ttacctttga
    10681 gattggtgtc tttggtgtat tccgcatcgg tgtaggtata agaagccgtc aagttaatat
    10741 tggcatttaa cgccgctttt gcttcaagtt ccacaccacg agcgcgaatc tcacctgctg
    10801 ggacttgcgc taatgggttt gttgggtctg ctgtcaggtt atttgttttc gtcaattgat
    10861 agactgcgcc cgtaacaacg accggcatat cattcggaac atattttacg ccagcttcat
    10921 actgttcacc tttcgatggc ttataggaaa tgcgcgggtt gctccacaga tcgaaagcac
    10981 tcggttcaaa ggactggcta tagctaaagt aaggtgaaat accattatcg aataagtaat
    11041 ttacaccacc gcgccaggtg aactgatgat cattgcgctc aatataacct tcagtcggcg
    11101 tataagagtt ttcacgaaca gtggtcgctt gcttagacca atcgtagcgt ccccccagag
    11161 tgaataccca tttattccat tccgcctgat cctgaacata aatacctgtt tgtttgcttt
    11221 cattcatctg gtatggctct gcgctaccaa atgcaaagta ttcagggtga tatttgttat
    11281 aaagatcgat ggatggtgcg gatccaaagc tggcattgat atcattacgc atccgcatga
    11341 agtctacccc tgtcagcaaa gtatgctcga cgtcacctgt agcaaactta ctttcaagtt
    11401 gggtatcaac gctaaagttt tgtagacgct cattatccac cactgtcccg cggtttaggg
    11461 tatgaccatc accagcaatc cctgtgccat aaacactttt ctgtgaggtt ttcatttcac
    11521 tgaaacgcag attctgacgc acggtgaagg tgtcattaaa accatgttca aaactatatc
    11581 ccaccatttt ctggttacgg gagtatgtat tattcgatgc accttcattg aagtcagtcg
    11641 gtagacgctt accattaggc aatggctcaa cggtcccctc tttcggcaac caaccgtaat
    11701 aacccgtttc tggttcattc tggaaatagg acaggaaagt aaaattggtt ttgtcgtctg
    11761 gacgccatga gaatgatgga gcaatggtat aacgctgaga ttcagagttt ttctgctgtt
    11821 cgtttgttga gcgagcgagg cccgtcaaac ggtaagagaa ctcaccgtta tcatccagtg
    11881 catcactgaa atcgaatccg gtctgaaaca gattatccgt ccccatttta aactgaattt
    11941 ctttcagggg ttcagtggtc ggacgcttac tcaccataga aataatccca ccaggattac
    12001 tttttccata gagaacagac gtcggcccac gcatcagttc aaccctctca agcatgtaag
    12061 gatcaatcac agcatcgtta taaaagttgc cctgaagttt tagtccatca aggtaattat
    12121 tttgattcag accaacagat gagaaaccac gaatgataac aaaatcatat gtattagaag
    12181 caccacgact gctaaccgta acgccaggtg tataacctaa tgcttctttt acagactgaa
    12241 attgacgcat ctgcatctct tcatttgtca caaccgaaat agactgcggc gttttttcaa
    12301 ttggtgtatc cgttttagtc gcagtagcag agtgcttcgc cgcaatagtt gcagcaggcc
    12361 cccatgcgct ttcttgcggc gcaggtgcag cggtaacggt gatagtgtct tctttcggtt
    12421 caaccgctgc ctgtgcataa acagacatgc cgctaaccgc tgtggctact acaactgcga
    12481 ttttacgcag tgagtgtttt ggctgagcag ttttggaacg cgccattggt atatctctga
    12541 tgtaaagtga atgataacgt aaacgagaat gattattata aagataagat aatattcgaa
    12601 acgctggcgg gttcgcaagc ggtacgcaat ctgagcgttc ctgaagggtt aataaacaac
    12661 cagatgaaaa gaaagggtta atactttagg tgggatgtta ttttaccgga tggcaattcg
    12721 ccatccggta tttcacgctt agatgttaat tactaccaaa catatccttg atccaaccgg
    12781 ctacaccgtc gctgtctttc tgctcttgct gagcaggttg ctgttgcggc tgttgctgcg
    12841 gctgagaaga ctgatcaaac ggattgcctg acggctgctg ctgcatctcg ctctgctggc
    12901 acagcgattg cggatcgctg gtccagaccg gcaagacacg catgccaccg ctgcaaacaa
    12961 agttgccgtc gtagtccacg cccatatctg caatatcttc tggcggaaca agattcagcg
    13021 gcgttggcgt ctggttagcc agataacgct gataaataga cattgccccg ctggcaccat
    13081 acagtttggt cggctggttg ttatcacggc ctacccaggt gatggtcacc gtgctgccgt
    13141 caatgcccgc aaaccaggta tctacgttat tgttggtagt ccctgttttc cctgccagat
    13201 gcaggttcgg gtatttcgcc ccaagttgac gacccgtacc acgttgtacc acctgctgca
    13261 tggtccatag tgtcagatac gccgcctgcg ccggaacagc gcgttctgcc tgcgggaagc
    13321 tctgatacag caccttgcca tcttccgcga ttaccgaacg cagtgcagaa agcggtgcac
    13381 ggttaccgcc gctggcgatg gtctggaatg cctgcgccac ttcgattggc gttaagttca
    13441 acgcccccag cagcatcgcc ggaaccggat gcagctgatc tttcggcacg cccagtttga
    13501 tccaggtctc cgtaactgca ggcagcccca gcgccatccc cagatttacc gtcggcacgt
    13561 tcatcgaacg ggtcaacgca tccaccagca tcactctgcc gctttcgcta taacgacggt
    13621 catcgttctg cggtgaccag acctggccat tcggctgacg cagcgcgatt ggcgcatcgg
    13681 caatccacgt gttcaggcga tagattttcg gctggcttaa agccgtcaga taagtcgctg
    13741 gttttgcaag ggaaccaatc gaacgacgcg cctgcatcgc acggttgtag cccgcaaact
    13801 gcggctcaga acctcccacc atcgcacgaa cttcaccact aaagcggtcg acgaccacaa
    13861 ttgcagtttc aagatcgctc aacttacgct gtttcttcag tgccggaatg ccttccacgg
    13921 cggctttttc tgccgcgtcc tgggccaccg agtcaaaggt agtgaagatc ttcacgccgg
    13981 agagatcttt taccttatcg cctaatttcg cctgcagctc ctgacgcacc agttgcataa
    14041 aggctggctg tggagagatt accccaccgc gcggctgtac gcccagagga cgcgcgctga
    14101 gcatttcata cagttcacca tcgataatct gctgttgttg tagcaaacgc agcaccagat
    14161 tacgtcgctc cagcgccagt ttcgggttac gccacgggtt gtagatggac gcccctttca
    14221 ccataccgac taacagcgcc tgctggtcga ggctgagctc ttctaccggg cgaccaaagt
    14281 aatacaagct cgcaagcggg aagccgcgga tttcgttgtc gccgctctga ccgagataca
    14341 cctcgttcat atacagctca agaatacggt ctttgctgta acgcgcgtcc atgatcagcg
    14401 ccatgtaagc ttcgttcgct ttacgccagt aagaacgctc gctggagagg aacaggtttt
    14461 tcaccagctg ttgcgtcagc gtactcgctc cctgcaccgt gcgtccggcg gtcaggtttg
    14521 ccagcaccgc acgtccgatg gagtagagac tgatcccatc atgctcgtag aaatgacggt
    14581 cttctgtcgc cagcaaagta tccaccagca agtccgggaa accactgcgc ggcacaaaca
    14641 gacgctgctc accgtttggc gaagagatca tggtgatcag gcgcggatca agacggaaga
    14701 aaccgaactg acggttgttc tccatattga cgatcgtcgc cagatgatcg ccatcaaagg
    14761 tcagacgcgc acgcacctgc ccttctttgc tgtccgggaa atcaaacgga cggcggatca
    14821 tctcaatgct gttggcctgc acggtaaatt cgccaggacg ggtcattttc gacacctgac
    14881 gatactgtgt tgcctccagc agcttcacca tttcgttctt gctgatggtc atatctggtt
    14941 caaggttgac cattcggcca taaactgccg cgggcagttg ccagaccttg ccatcaatac
    15001 ggctacgaat tttttgatcg agataaacgc cgtaaatggc gatcagcacg gcaaaaacga
    15061 tagccagttt taacagcagc catagccagc cgcgtttgcc acgaggctta cgccctttgc
    15121 ctttgccctt acctttgcgc ggcatcggtt cttcatcctc atagtcatca taatcgtcgt
    15181 aatcatcgtc atcttcgtaa cgacgacggc ttaccttttg tttgaccgga cgcgtcggtt
    15241 tccctttgcg tccaattggc tcgcggtcat tcccggccat gctttttctc cgcaatattc
    15301 aggcgcaaaa gcccgatttt ctgttcttct gttacaagac agaagaagat atctctcaaa
    15361 atttgtgctc gatcggtccc ctctcccttg agggagaggg ttagggtgag ggtgaacgtg
    15421 cgcgctgatg ccctcacccc ggccctctcc cacagggaga gggagaaaac caaccttacg
    15481 aatacttttt cgtccgtcgc gtcggtgccg tgtttgccgg gtcgtccggc cagacgtgtt
    15541 tgggataacg ccctttcatc tctttttgca cctcacggta cgctcctttc cagaaggcgc
    15601 tcaaatctcg tgtgatttgt agtggccttt gggcgggtga aagcaactcc agcaccagcg
    15661 gcacgccccc ctgggcgata gtcggattag tggcctcacc aaacatctcc tgcattctca
    15721 ccgccagtgc gggcgggtta tcttcatgat aacgaatggc gatccggctt cccgtcggca
    15781 cagtgtaatg cgcaggcaat tcactatcca gacgttgctg cattccccaa tcaagtaatc
    15841 cgcgtagtgc ctgataaatg tccagtgatt tcaggccgcg tagtgaatgt acgcctgtca
    15901 tatgtggcag cagccacgtt tccagcgctg ccaataaact ttcatcatca accgctggcc
    15961 agtcatattc cggcaaccac tttgcggcgc ataacaaacg caagcgtagc tgttccgctt
    16021 ccgccgtcca gttgagcacg cttaaacctt tatcacggat gccattaagc atcgcctgat
    16081 gcaactcgtc ttctgacggt ttcgccagcg gctgcacttt caccgtcaac tgaccgattt
    16141 gtagccgacg ccaggctttc agcgtacctt gcgcgtcatc ccattccacg gtgtcagatt
    16201 gctgcaccag ctgcggacag cgttgtacta attcatcaat atcgaccggt agcgccagta
    16261 aaatccgcgc atccggcgag gcgctgccct gcaataataa cggtgcgatc aaccattcgt
    16321 ggcggcttag cgcgtcgtcg gcatcgagca tcgctcccat gccgtttgcc agttgatagc
    16381 gtccatcttg cccacggcga tgagcaatgc gatcggcaaa cgccccggca agtagcggcg
    16441 cgataagcga actgtctgcc tcaccgccgc gtacgtttaa gcgttttaac agttgctgac
    16501 tacgttgctg ccaggctggt tgattgcgcg aaaacgccac gcccaggtca ctattgccca
    16561 tcagtggcgg ctcttcgaga atggcggcaa tttttgccgc ggtagcagct tcgtcgtcgt
    16621 tcttcgcgct aaccagcatt gccgctaaac gcggatcgtt acccagcgct gccatttttt
    16681 gcccttgcgc actaagccgt tcaccgtcca gcgcccccag catttgtaac agacgtttcg
    16741 cggccattag attcactact ggcggttgat ccagccagct catctgcgcc ggatcgctgc
    16801 atccccattg cagtaattcc atcagcaaac cggaaagatc gctttgtaag atctccggtt
    16861 cactttgcgc ggcagcgcgt tctgcttgtt ctttggcgat taaatgcagg cagatacccg
    16921 gctccagacg cccggcgcgc ccggcacgct gcgtcatgga tgcctggcta acgcgttgag
    16981 taatcagtcg cgtaagcccc gtgcgcggct caaaacgcgc cacacgctcc tgggcacaat
    17041 ccaccaccag acgaatacct tcaatggtta aactggtttc agcaatattg gtcgccagca
    17101 ccactttgcg catcccttgc ggtgccggga ggatcgcttt tcgctgatcg ttcagcgaca
    17161 acgcgccata cagcgggcag agcaacacat cactgccgat gcgcgaagcc agttgttcct
    17221 gcacacgctg aatttctccg acgccaggta aaaataacag taatgatccg ctttcctgac
    17281 gcagcatttc ggcggtggct accgcaacgg cttcgtcaaa acgctgatgc gcgggcagcg
    17341 gtaaataatg gcgttcaacc ggaaacgagc gcccttctga gatgacgacg ggcgcttctg
    17401 gcagcatttg ctgcaagcgg tcgttatcca gcgtagccga cataatcagc agtttaaggt
    17461 catcacgcag accttgttgc acatcgagta acagcgccaa cgccaaatcc gcctgcaagc
    17521 tgcgctcatg aaattcatca aggatcacca atccaacacc gctcaactcc gggtcacgct
    17581 ggatcatccg cgtcagcacg ccttcggtaa ctacttccag acgggtattt ggcccgacgc
    17641 agttttgcgc acgcatccgg tagccaacgg tatcgcctgg cttttcgtta agcagctccg
    17701 ccagccgttg cgcgacgtta cgcgccgcca gacgacgcgg ctccagcagg ataattttcc
    17761 cgttaatgcc gggatgcgcc agcagttgca gcggcagcca ggttgatttc ccggccccgg
    17821 tcggcgcact taataatacc tgcggcgcac aatcgagagc ggtaagtaat tcaggtaaga
    17881 cggcagcaac gggcaacgac gacacaaaac gctccagagg gttaacattc ttcgcgccac
    17941 attgtagcat cgcggtaatt cataaccgag tgcctcacat gtctgaaccg caacgtctgt
    18001 tctttgctat cgacttacct gcagaaatcc gcgaacagat tatccactgg cgcgccacac
    18061 acttcccacc tgaggcggga cgtccggtcg ccgccgataa tttgcatctg actctggcat
    18121 ttttaggcga agtgagcgcc gagaaagaga aggcgctttc tcttttagcc ggacggattc
    18181 gtcaacctgg tttcacactc acgcttgatg acgccggaca atggctgcgt tcgcgtgtgg
    18241 tgtggttagg gatgcgtcag ccgccacgcg gcttaatcca gctggcgaat atgctccgtt
    18301 cacaggctgc ccgcagcggt tgttttcaaa gcaatcgtcc gtttcatcca catattacct
    18361 tattgcgcga cgccagcgag gcggtgacaa tcccgccgcc aggttttaac tggtcttatg
    18421 cggtgacgga gttcaccctt tacgcctcct cgtttgcccg tggacgcaca cgctacacgc
    18481 cgctaaaacg ctgggcgcta acgcaataac aaggattgtc gcaatggaat tctctccccc
    18541 tctacagcgc gcgacgctaa ttcagcgtta caaacgtttt ttagccgatg tgatcacacc
    18601 cgatggtcgc gaattaacgc tacactgccc gaatacgggt gcgatgaccg gttgtgcaac
    18661 gcgtggcgat accgtctggt attcgacttc agacaacacc aaacggaaat acccacacac
    18721 ctgggaacta actcaaagcc agagcggcgc atttatttgc gtcaatacgc tttgggctaa
    18781 caggttgacg aaagaggcta tccttaatga atcaatttca gaactgtcag gctatagctc
    18841 gctgaaaagc gaagtaaaat acggcgcaga acgcagccgt attgacttta tgttgcaggc
    18901 ggattcgcgt ccagactgct atattgaagt gaaatcggtt acgttagcgg agaacgaaca
    18961 gggatatttt cccgatgcgg tcactgaacg aggtcagaaa caccttcggg agttgatgaa
    19021 cgtagcggct gaaggccagc gtgcggttat ctttttcgcc gtgctgcatt cagccattac
    19081 acggttttca cccgcgcgcc acatcgatga gaaatacgcg caactattgt cagaagctca
    19141 acagaggggg gtagaaattc tggcttacaa agcggaaatt tctgctgaag gcatggctct
    19201 taaaaaatca ctgtcggtta cattgtagta aagtaagtaa ctggttaatt tacattctgg
    19261 tcgcgtgcgc aaatacgctt ttcctcacac agttgtcaag tgttacgttt agataattgc
    19321 tatccggaaa agcatctgct atttatagcg gcctcatttt tcccccgaac atggggatcg
    19381 atagtgcgtg ttaaggagaa gcaacatgca agaagggcaa aaccgtaaaa catcgtccct
    19441 gagtattctc gccatcgctg gggtggaacc atatcaggag aagccgggcg aagagtatat
    19501 gaatgaagcc cagctggcgc acttccgtcg tattctggaa gcatggcgta atcaactcag
    19561 ggatgaagtc gatcgcaccg ttacacatat gcaggatgaa gcagccaact tcccggaccc
    19621 ggtagaccgt gcagcccagg aagaagagtt cagcctcgaa ctgcgtaacc gcgatcgcga
    19681 gcgtaagctg atcaaaaaga tcgagaagac gctgaaaaaa gtggaagacg aagatttcgg
    19741 ctactgcgaa tcctgcggtg ttgaaattgg tattcgccgt ctggaagcgc gcccgacagc
    19801 cgatctgtgc atcgactgca aaacgctggc tgaaattcgc gaaaaacaga tggctggcta
    19861 attacagccg ttccatcacg tttaccacag gcgggaaatg ctcccgcctt attttttgtt
    19921 caaagagatg acagacacac agtatattgg ccgcttcgcc ccctctcctt ccggcgagct
    19981 tcattttggc tctctgatcg ccgcgctcgg cagctatttg caggctcgcg cccggcaagg
    20041 tcgctggctg gtacgcatag aagatatcga cccgcctcgt gaagttcccg gtgccgcaga
    20101 aactatcctg cgccagctgg aacattacgg tctgcactgg gacggcgatg ttctctggca
    20161 atcgcaacgt cacgacgcct atcgtgaagc actcgcctgg ttacatgaac aaggactaag
    20221 ttattactgc acctgtacgc gtgcgcgtat tcaaagcatt ggcggtattt acgacggtca
    20281 ttgccgggtg ttgcatcatg gaccagacaa tgccgcagtg cgtatccgcc agcagcatcc
    20341 ggtcacgcaa tttactgacc agctgcgcgg cattattcac gccgacgaaa aactggcacg
    20401 ggaagatttt atcattcatc gccgtgatgg gttgttcgcc tacaacctgg ctgttgtggt
    20461 tgatgatcat ttccagggcg ttacagaaat agtgcgtggg gctgatctga ttgaaccaac
    20521 agtaaggcaa atctcgctgt accagctttt tggctggaaa gtgccagatt acattcatct
    20581 gccgctggcg cttaatccac aaggcgctaa actttccaag cagaatcatg cgcctgcgtt
    20641 gccgaaaggc gatccacgtc cggtactaat cgcggcactt caatttctgg gtcagcaggc
    20701 agaagcacac tggcaggatt tcagcgtcga acaaatcctt cagtcagcag tcaaaaactg
    20761 gacgctgacc gccgtgcccg agtcggcaat tgtaaattca acattctcaa atgcgtcatg
    20821 ctgagctatg attagccgct atttttttgt cctgaatgat gtttgacact accgaggtgt
    20881 actattttta cccgagtcgc taatttttgc cgcaaggtgc taagccgcga ggaaagcgag
    20941 gctgaacagg cagtcgcccg tccacaggtg acggtgatcc cgcgtgagca gcatgctatt
    21001 tcccgcaaag atatcagtga aaatgccctg aaggtaatgt acaggctcaa caaagcggga
    21061 tacgaagcct ggctggttgg cggcggcgtg cgcgacctgt tacttggcaa aaagccgaaa
    21121 gattttgacg taaccactaa cgccacgcct gagcaggtgc gcaaactgtt ccgtaactgc
    21181 cgcctggtgg gtcgccgttt ccgtctggct catgtaatgt ttggcccgga gattatcgaa
    21241 gttgcgacct tccgtggaca ccacgaaggt aacgtcagcg accgcacgac ctcccaacgc
    21301 gggcaaaacg gcatgttgct gcgcgacaac attttcggct ccatcgaaga agacgcccag
    21361 cgccgcgatt tcactatcaa cagcctctat tacagcgtag cggattttac cgtccgtgat
    21421 tacgttggcg gtatgaagga tctgaaggac ggcgttatcc gtctgattgg taacccggaa
    21481 acgcgctacc gtgaagatcc ggtacggatg ctgcgcgcgg tacgttttgc cgccaaattg
    21541 ggtatgcgca tcagcccgga aaccgcagaa ccgatccctc gcctcgctac cctgctgaac
    21601 gatatcccac cggcacgcct gtttgaagaa tcgcttaaac tgctacaagc gggctacggt
    21661 tacgaaacct ataagctgtt gtgtgaatat catctgttcc agccgctgtt cccgaccatt
    21721 acccgctact tcacggaaaa tggcgacagc ccgatggagc ggatcattga acaggtgctg
    21781 aagaataccg atacgcgtat ccataacgat atgcgcgtga acccggcgtt cttgtttgcc
    21841 gccatgttct ggtacccact gctggagacg gcacagaaga tcgcccagga aagcggcctg
    21901 acctatcacg acgctttcgc gctggcgatg aacgacgtgc tggacgaagc ctgccgttca
    21961 ctggcaatcc cgaaacgcct gacgacgtta acccgcgata tctggcagtt gcagttgcgt
    22021 atgtcccgac gtcagggtaa acgcgcatgg aaactgctgg agcatcctaa gttccgtgcg
    22081 gcttatgacc tgttggcctt gcgagctgaa gttgaacgta acgctgaact gcagcgtctg
    22141 gtgaaatggt ggggtgagtt ccaggtttcc gcgccaccag atcaaaaagg gatgcttaac
    22201 gagttggatg aagagccgtc accgcgtcgc cgtactcgtc gtccacgcaa acgcgcaccg
    22261 cgtcgtgagg gtaccgcatg acagtggcgt atattgccat aggcagcaat ctggcctctc
    22321 cgctggagca ggtcaatgct gccctgaaag cattaggcga tatccctgaa agccacattc
    22381 ttgccgtttc ttcgttttac cgcactccac cgctggggcc gcaagatcaa cccgattacc
    22441 tcaacgccgc cgtggcgctt gaaacttcgc tcgccccgga agagttactc aaccatacac
    22501 agcgcataga actgcaacag ggtcgtgttc gtaaagctga acgctgggga ccacgcacgc
    22561 tggatctcga tatcatgctg tttggtaatg aagtgataaa taccgaacgc ctgaccgttc
    22621 cgcactacga tatgaagaat cgtggattta tgctgtggcc gctgtttgag attgcaccgg
    22681 agctggcgtt tcctgatggg gagacgttgc gtgaagtctt acatacaaga gcatttgacg
    22741 aattaagcaa atggtaattt atttatatta cgaatataaa tattttctga aaatcataca
    22801 tcctctcttt ccttaataaa aattttagtt taaacatttt tgcagaaaca taatataaat
    22861 tcccgcagat aaatatcaat cccatcgcca taccctaacc aattgcactt gtgaatttat
    22921 ttttgcttaa ttttgaatta agaaaattta atttgttcaa aagcccttaa agtgcacaat
    22981 aggcgtccta atgaacaatg gttgtaaatt ttggtaacaa atatcattgc tcattaatat
    23041 ctagcatctg cttataacat ttgtgtcatg aatagtcatt cttcaaacca gtagtctgac
    23101 taatggaatt ttattcaagg atttttatgt ctaaaaaatt aggttttgcc ctgtctggtc
    23161 ttatgctggc aatggttgca ggtactgctt ctgctgatat ggatggtggt cagttaaata
    23221 tcagtggtct ggttgttgat aacacctgtg aaacccgcgt tgacggcggt aataaagatg
    23281 gtctgatcct gctgcaaact gcaactgttg gtgaaatcga tgctggcgtg ctgaatgaca
    23341 ccgttggcgc taaagccaaa cctttcagca tcaccgttga ctgcagcaaa gcaaatccga
    23401 atccaggtag tacagctaaa atgaccttcg gttctgtttt ctttggtaat agcaaaggaa
    23461 cgctgaacaa cgacatgtct attaacaacc cgtctgacgg cgtgaatatc gctctgcaca
    23521 atattgatgg ttccactatc aaacaggttc aaattaacaa ccctggcgat gtgtatacta
    23581 aagccctgga tgcaacgaca aaatctgctg tttatgattt taaagcgtct tacgttcgtg
    23641 ccgtcgcaga ccaaacagca actgctggtt atgtaaaaac taacactgca tacaccatta
    23701 cttatcagta agtattcacc aattaaattg attgaatgaa tatacaggga atagtgttaa
    23761 taatttctat ttttatatta ttccctgttt taattaactc tgtcagggat ggtttatgtt
    23821 ttttaacacc aaacatacaa cagctttatg cttcgtaacc tgtatggctt ttagttcatc
    23881 gtctattgcg gacattgtca tttcgggtac tcgcgtaata tataaaagcg atcaaaaaag
    23941 tgtcaacgta cgtctggaaa ataaagggaa taacccgttg cttgtccaga gttggttaga
    24001 tactggcgat aacaacgctg agcctggcag tattacagtc ccttttactg ccacgccgcc
    24061 agtatcgcgt attgatgcca aacgtgggca aacaatcaaa ttaatgtaca cagccagcac
    24121 ctcactgcct aaagacagag agagcgtgtt ctggtttaac gtactggaag ttccaccaaa
    24181 accagatgca gaaaaggtcg cgaatcaaag tctgctgcaa ctggcatttc gcacacgtat
    24241 aaaacttttc tatcgcccgg atggattgaa gggaaatccc tctgaagccc cgttagccct
    24301 gaagtggttc tggtcaggtt cagaaggtaa ggcgtcattg cgagtgacca atccaacccc
    24361 ttactacgtc tcttttagca gtggtgattt agaagctagc ggtaaacgct atccgattga
    24421 tgtgaaaatg attgcaccat ttagtgatga ggtcatgaaa gtcactggcc ttaatggcaa
    24481 agcaagttct gcaaaagtac atttttacgc cattaatgat tttggtggcg ccattgaagg
    24541 taatgccagt ctgtaatcag gcaaggatat aattccgcag gaagcataac gtgactataa
    24601 aaccaactaa aaattatcat aatcatctga cccgtatcgc tacgttttgc gcgctgctgt
    24661 attgcaatag tgctttctgt gctgaacttg ttgaatatga ccataccttc ctgatggggc
    24721 agaatgcctc aaatattgat ctcagccggt atagtgaagg taaccctgct ataccgggta
    24781 tgtacgacgt cagtgtttat gtcaacgatc aacctattat taaccaaagc attacattta
    24841 ttgaaattga aggaaaaaaa acgcacaagc ctgtatcaca cttaagaatt tattgcagtt
    24901 tcatataaat tctcccgata taaataacga aaaagccgtt ctgcttgcca gggatgaaac
    24961 actcggcaat tgccttaatt tgacggaaat tatccctcag gcttctgttc gttatgacgt
    25021 taacgagcaa cgtctggata tagacgttcc tcaggcatgg gtaatgaaaa attaccaaaa
    25081 ctatgttgat ccatcgttat gggaaaatgg cattaatgcg gccatgttgt catacaacct
    25141 caacggatat catagtgaaa ctcctggtcg aagaaatgac agcatttatg ctgcttttaa
    25201 cggtgggatg aatttaggtg catggcgact gcgtgcctca ggcaactaca actggatgac
    25261 cgattctggc agtaattatg attttaagaa tcggtatata cagcgtgata tcgcctcgct
    25321 gcgttctcaa ctcattcttg gtgagtctta tacaacgggc gaaacctttg attctgtcag
    25381 tatccgcggc attcgtttat acagcgacag ccgcatgttg cctccgacat tagcgagttt
    25441 tgcgcctatc attcatggcg ttgctaatac caacgccaaa gtcactatta cgcaaggtgg
    25501 ctataagatt tatgaaacga ctgtgccgcc aggcgctttc gtcattgatg atctgagtcc
    25561 gtcaggttac ggcagcgatc ttattgttac tgtcgaagaa tccgatggct caaagcggac
    25621 attctcgcaa cctttttcat cggtcgttca aatgttacgc cctggcgttg gacgttggga
    25681 tattagcggc ggccaggtct taaaagatga tattcaggat gagcctaatt tatttcaggc
    25741 cagctactac tacggtctga ataactatct aacg
//
LOCUS       DAESHI010000051        25637 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.051, whole genome shotgun sequence.
ACCESSION   DAESHI010000051 DAESHI010000000
VERSION     DAESHI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25637
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.051"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(65..796)
                     /gene="rlmB"
                     /locus_tag="KW011_002219"
     CDS             complement(65..796)
                     /gene="rlmB"
                     /locus_tag="KW011_002219"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBH7660541.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(976..3417)
                     /gene="rnr"
                     /locus_tag="KW011_002220"
     CDS             complement(976..3417)
                     /gene="rnr"
                     /locus_tag="KW011_002220"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBH7660542.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3456..3881)
                     /gene="nsrR"
                     /locus_tag="KW011_002221"
     CDS             complement(3456..3881)
                     /gene="nsrR"
                     /locus_tag="KW011_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HBH7660543.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4086..5384)
                     /gene="purA"
                     /locus_tag="KW011_002222"
     CDS             complement(4086..5384)
                     /gene="purA"
                     /locus_tag="KW011_002222"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBH7660544.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5488..5685)
                     /locus_tag="KW011_002223"
     CDS             complement(5488..5685)
                     /locus_tag="KW011_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HBH7660545.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5767..6771)
                     /gene="hflC"
                     /locus_tag="KW011_002224"
     CDS             complement(5767..6771)
                     /gene="hflC"
                     /locus_tag="KW011_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HBH7660546.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6774..8033)
                     /gene="hflK"
                     /locus_tag="KW011_002225"
     CDS             complement(6774..8033)
                     /gene="hflK"
                     /locus_tag="KW011_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HBH7660547.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8119..9399)
                     /gene="hflX"
                     /locus_tag="KW011_002226"
     CDS             complement(8119..9399)
                     /gene="hflX"
                     /locus_tag="KW011_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBH7660548.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9476..9784)
                     /gene="hfq"
                     /locus_tag="KW011_002227"
     CDS             complement(9476..9784)
                     /gene="hfq"
                     /locus_tag="KW011_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBH7660549.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9870..10820)
                     /gene="miaA"
                     /locus_tag="KW011_002228"
     CDS             complement(9870..10820)
                     /gene="miaA"
                     /locus_tag="KW011_002228"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBH7660550.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10813..12660)
                     /gene="mutL"
                     /locus_tag="KW011_002229"
     CDS             complement(10813..12660)
                     /gene="mutL"
                     /locus_tag="KW011_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBH7660551.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12670..14007)
                     /gene="amiB"
                     /locus_tag="KW011_002230"
     CDS             complement(12670..14007)
                     /gene="amiB"
                     /locus_tag="KW011_002230"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HBH7660552.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14026..14487)
                     /gene="tsaE"
                     /locus_tag="KW011_002231"
     CDS             complement(14026..14487)
                     /gene="tsaE"
                     /locus_tag="KW011_002231"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBH7660553.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14459..16006)
                     /gene="nnr"
                     /locus_tag="KW011_002232"
     CDS             complement(14459..16006)
                     /gene="nnr"
                     /locus_tag="KW011_002232"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBH7660554.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16005..17144
                     /gene="queG"
                     /locus_tag="KW011_002233"
     CDS             16005..17144
                     /gene="queG"
                     /locus_tag="KW011_002233"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBH7660555.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17127..17180)
                     /locus_tag="KW011_002234"
     CDS             complement(17127..17180)
                     /locus_tag="KW011_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660556.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17414..17489)
                     /locus_tag="KW011_002235"
     tRNA            complement(17414..17489)
                     /locus_tag="KW011_002235"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17454..17456),aa:Gly,seq:gcc)
     gene            complement(17525..17600)
                     /locus_tag="KW011_002236"
     tRNA            complement(17525..17600)
                     /locus_tag="KW011_002236"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17565..17567),aa:Gly,seq:gcc)
     gene            complement(17758..17833)
                     /locus_tag="KW011_002237"
     tRNA            complement(17758..17833)
                     /locus_tag="KW011_002237"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17798..17800),aa:Gly,seq:gcc)
     gene            complement(18044..18589)
                     /gene="orn"
                     /locus_tag="KW011_002238"
     CDS             complement(18044..18589)
                     /gene="orn"
                     /locus_tag="KW011_002238"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HBH7660557.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18684..19736
                     /gene="rsgA"
                     /locus_tag="KW011_002239"
     CDS             18684..19736
                     /gene="rsgA"
                     /locus_tag="KW011_002239"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HBH7660558.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19833..20801
                     /gene="psd"
                     /locus_tag="KW011_002240"
     CDS             19833..20801
                     /gene="psd"
                     /locus_tag="KW011_002240"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HBH7660559.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20823..24146
                     /gene="mscM"
                     /locus_tag="KW011_002241"
     CDS             20823..24146
                     /gene="mscM"
                     /locus_tag="KW011_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HBH7660560.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24297..>25637)
                     /gene="yjeM"
                     /locus_tag="KW011_002242"
     CDS             complement(24297..>25637)
                     /gene="yjeM"
                     /locus_tag="KW011_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HBH7660561.1"
                     /translation="LMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIW
                     MVSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKI
                     ARITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVV
                     FAIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVL
                     SNGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFT
                     LCYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASA
                     FFNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVT
                     FANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
BASE COUNT         5928 a         7193 c         6501 g         6015 t
ORIGIN      
        1 tatcttacca gctatagaca aaaaaaccat ccaaatctgg atggcttttc ataattcaga
       61 cgaactagct gcgctgacga accgcttcaa ataagcaaat tccggttgca accgaaacgt
      121 tcagggaaga aacacttcct gccattggga tgctgatcaa ctcatcgcaa tgttcacggg
      181 tcaggcgacg cataccttca ccttccgcgc ccatcaccag cgccaagcgt ccggtcattt
      241 tgctctgata gagtgtatga tccgcctcgc ctgccgtacc gacgatccag atattctctt
      301 cctgcaacat acgcatggtg cgcgcaaggt tagtcacccg aatcagtgga acgctttctg
      361 ccgcgccgca ggctactttt ttcgccgtag cgttgagctg tgcggagcga tctttcggca
      421 caatcaccgc gtgaacgcca gcagcgtccg cgctacgcag gcacgcgccg aggttgtgcg
      481 gatcggttac accgtcgagg atcagcagga acggttgatc gagcgaagcg atcagatccg
      541 gcagatcgtt ttcctgatac tgacgtcctg gcttcacgcg ggcgataatg ccctgatgca
      601 cggcaccgtc gcttttctcg tcgagatatt ggcggtttgc caactggata accacgccct
      661 gagactcaag ggcgtgaatc agcggtaaca gacgtttatc ttcacggcct tttaaaataa
      721 agacttcctg aaaacgttca ggggcgcgct ccagcagggc ctgcactgcg tggatgccgt
      781 aaatcatttc gctcattaat gtactcgttg tttacgtttt gcctgatgca ctacgtttat
      841 ccggcctaca tgatctctgc aatatattga atttgcgtga ttatgtaggc cggataaggc
      901 gttcacgccg catccggcat gaacaaagcg cattttgtca gcaatctaac cctcttcttt
      961 taaagagggt attgatcact ctgccacttt tttcttcgcc gcacgcttcg ctttggtcgc
     1021 tgcggctatt ttctgcgttt tcgccgatgg ctttttcgct tttctcgcgt ctttcttcgc
     1081 cgctttcggc ttcgtttttt tctcaccgcg gaaggcgctg tctggctcaa agtttacctt
     1141 tttaccgacc tgacgacgct tgccgccttt tttgcctgca tcgccttttt tcgctttctc
     1201 gcgcgccgtt ttaccgacgt tgcgcggtgc gcgttcactg gagatcagac taaagtcgat
     1261 tttgcgctcg tccatattaa ccgcttcgac gcgaacttcc acgcgatcgc ccaggcgata
     1321 agtctggccg ctggattccc ccatcaggcg ttgccctacc tggtcaaagc gatagtagtc
     1381 attgtccagc gaagagacat ggaccagacc atcaatgaac aagtcgtcca gacggacgaa
     1441 gaagccaaag ccagtgacgc tggaaattac gcctttaaag acgttaccta cctggtcgag
     1501 catgaagtca cacttcagcc agtcagcgac atcgcgcgtt gcttcgtcgg cacgacgttc
     1561 cgccatcgaa cagtgctgac ccagttgcaa catctcttcc atcgaataat ggtagccgcc
     1621 ggtttcagtg gtgttgccct gatgcccctg ctctttcgcc agcagatact taatggcgcg
     1681 gtgcagcgtc aggtcaggat aacgacgaat cggcgaagta aagtgcgcat aggactgcaa
     1741 tgccaggcca aagtgaccac ggttttctgg atcgtaaatc gcctgtttca tcgagcgcag
     1801 cagcatggtt tgcagcattt ctgcatcagg acggtcggca accgactcca gcagttccgc
     1861 gtagtcacgc ggttccggct tgttaccacc cggcagctcc agccccagct ccgccagcac
     1921 tgaacggaaa gaggtaatcg cttcggtgct cggcttgtcg tgaatacgga acagtgccgg
     1981 ttctttcgct ttctcaacga aacgcgccgc cgagatattc gccagaatca tgcactcttc
     2041 aattaatttg tgcgcgtcgt tacgctgggt ctgttcgata cgttcaatac ggcgttcagc
     2101 gttgaaaatg aacttcgctt cttcgctctc aaatgagatc ccaccgcgtt cttcacgggc
     2161 tttatccagc actttataga ggttatgcaa ctcttcgaga tgcttaacca gcggggcgta
     2221 ctgctcgcgc agatcctgat cgccctgcag aatatgccag actttggtgt aggtcagacg
     2281 cgcgtgagag ctcatcaccg cttcgtagaa tttgtagccc gtcaggcggc ctttcgacga
     2341 aaccgtcatc tcgcacacca tacacaggcg gtctacctgc gggttgagcg aacacaggcc
     2401 gttagagagc acttccggca gcatcgggat aacctgcgaa gggaagtaca ccgacgtgcc
     2461 acggttacgc gcttctctgt ccagcggcgt tggcggacgc acatagtagc tgacgtcggc
     2521 aatcgcgacc cataaacgcc agccgccgcc gcgttttttc tcgcagtaaa ctgcatcgtc
     2581 aaagtcacgg gcgtcttcgc catcaatggt gaccagcggt aaatcgcgca aatcgacacg
     2641 gcccgctttt gcttcttccg gcacttcttc tttcagcccc gcaacctgtt gctcaacagc
     2701 ctgcggccag atatacggaa tttcatgggt acgcagagcg atatcaaccg ccatgccggt
     2761 gcccatattg tcgcccagca cttcgacgat tttacccacc gctttggtgc ggcgagtcgg
     2821 acgctgagtc agttcgacta cgaccacaaa gcccatccgc gcgcccatga tctgatcggg
     2881 cgggattaag atatcgaagc tcagacggct atcgtcagga accacaaagc cgacgcccgc
     2941 ttcggtaaag tagcgaccaa caatctggct ggtttttggc accagtacgc ggacaatacg
     3001 cgcttcacga cgacctttac ggtcagcacc cagcggctgc gccagcacct gatcgccatg
     3061 aatacaggtt ttcatctgct cgctggagag atacaaatca tctttacgcc cttcaacccg
     3121 cagaaagccg tagccatcac ggtggccaat aacggtacct ttcaccaggt cgaggcgttc
     3181 cggcagcgca tagcactgac gacgagtgaa gaccagttga ccatcgcgct ccatcgcgcg
     3241 caggcggcga cgcaggcctt caagctgctc ttcgccttca atgtgcagtt ctaccgccag
     3301 ctcatcacgg ctggccggtt tttcacgttt ggttaaatgt tcgaggataa attcccgact
     3361 agggatggga ttcgcgtatt tttcagcttc gcgttcctgg aaaggatctt gtgacatctc
     3421 ggttcctccg ttgtcatctc tgatgaagat tttcgtcact ccaccagcaa taatttataa
     3481 agcggttgat tctcttcaac caaatcggca agcgtgtagt tatccagttc cgtaagaaaa
     3541 ctttgcacgg ccttagaaag cgcctgtttc aaccgacagg caggtgtaat gtggcaaaac
     3601 tcactgctgc aattcaccag cgataagggc tccagctcgc gcaccacatc accaatacgt
     3661 atcgcactcg ccggtttacc caggcgaatg ccgccatttt ttccacgaac agcagtcacg
     3721 tagccggcac gactaagttg attgattatt ttgaccatat gattacggga gacgccgtag
     3781 acgtcagtca cttcagaaat actggtcatc cgcccttctg gcaatgacgc catgtagatc
     3841 agcgcacgta atccgtaatc agtgaaactc gttaactgca catcaacctc aaaagggaaa
     3901 tcgggaaaaa atacatttat attgatgata aaccagccac aagctgtgtc gctaatttat
     3961 ttcagttcgg ggaaggaaaa aagcgaggat ttaacactgt gtcggatagc gtaatggctt
     4021 aaccgacaca gcaatgtatc acactgttag cccggcaggc aaaatatctg ccaggcgtac
     4081 cagaattacg cgtcgaacgg atcgcgcaga atcatggttt cagtacgatc cggaccggta
     4141 gagatgatat cgatcggcac accggtcagc tcttcaatac gcttgatgta gttcagcgcc
     4201 gcctgcggca ggccgctacg atctttcacg ccgaaagtgg attcagacca gcccggcatg
     4261 gtttcgtaaa tcggctctac acctttccag tcgtcagctg ccagcggagt ggtagccact
     4321 tcgcgaccat ccggcatacg gtaagccacg cagagtttaa cctctttcag gccatccaga
     4381 acgtccagct tggtcaggca gaagccagac agggagttca gctgtaccgc acgacgaacg
     4441 gcaacggtgt ccagccagcc ggtacgacga cgacgacccg tagttgcgcc gaattcgtta
     4501 ccctgcttgc agaggaactc gccagtttca tcaaacagtt cggtcgggaa cggacctgca
     4561 cctacacgag ttgagtaagc tttgaggata cccagaacat aatcaacata acgcgggccc
     4621 aggccggaac cggtcgccac gccaccagca gtggtgttgg aagaagttac gtacggataa
     4681 gtaccgtggt cgatatccag cagcgtaccc tgcgcacctt caaacatgac gaaatcgcca
     4741 cgctgacgcg cctggtcgag caggtcagaa acgtcaacca ccatagaagt caggatgtcg
     4801 gcaacagcca tcgtatcatc cagaactttc tggtaatcaa ccgcttcagc tttgtagtag
     4861 ttaaccaact ggaagttgtg atattccatc acttctttca gtttttcagc gaaggtttct
     4921 ttgtcgaaaa ggtcgccaac acgcagaccg cgacgtgcta ctttatcttc ataagcaggc
     4981 ccgataccac gaccggtggt gccgatcgct ttcgcgccac gcgctttctc acgcgcgtta
     5041 tccagcgcaa cgtgataatc aaggatcagc ggacatgctt cagacagcag cagacgctca
     5101 cgaacgggga tgccacggtc ttccagttct ttcatctctt tcatcagcgc ggccggagac
     5161 agcacaacac cgttaccgat gatgctggtt acattctcgc ggagaatacc tgatggaata
     5221 agatggagaa cggttttttc accgttgatt acgagagtat ggcctgcgtt gtgaccgccc
     5281 tggtagcgta caacatattt agcccgttca gtcagaagat cgacgatctt acctttacct
     5341 tcgtcacccc attgggtgcc cagtacgacg acgttgttac ccatttttca aaatcaccgt
     5401 ttgcttaaaa atggattcta ccatcgcttt ttcagagtta cagcactttt tgcactcaaa
     5461 aatgacctcg atcagtctgt tttttgttca gccaatcgtt ttcctcaaca tgtagtagac
     5521 cacaacgccc gcaaccacaa gtccaccgcc aaaacgacgt aaaatattat cgggcaaatt
     5581 ggtcatcgca gagatcatct tcttccatgc cttcgggtaa agcatcggcc ctaaaccttc
     5641 cagtaccaaa accagggcaa gcgccagcca gattgtcgaa ttcattcatt atccttatag
     5701 aaaaagaaaa ccaccgacat ccctgaggat gcggtggctt tattgacctg taccgcagtc
     5761 gttatattaa cgcgttgcgg aagtcggcgt cttcatgtag cggaagaaat cgctatccgg
     5821 gctcatgacc atcacgtcct gattgccaga gaagctgttc tcataagcac gcaggctacg
     5881 gatgaatgcg tagaagtccg gatctttact gaatgcatca gcaaacagtt tggctgcttc
     5941 ggcatcgcct tcaccacgca tgatgcggcc ctgacgctca gcttctgcca gcgttctggt
     6001 cacttcatag tcggcagtcg cgcgcagttt ttccgcttct tcctgacctt gtgaacggtg
     6061 acgacgcgct accgcttcac gctcggcgcg catacggttg tagatcgctt cagacacttc
     6121 ggtcggcagg ttgatctgct tgatacgcac atcgacaact tcaataccca gcgccgccat
     6181 actgttcggg ttgatgaccg gaactttgcc cttcgtctca gccgttacgc gctctgccgc
     6241 ttcggcaatg gcgttatctg ccgccggggt agtaacttca tcttctgtac ccgcagaacc
     6301 ggagttcagc gcgtcacgta cttcgagggt cagacgacca cgggaatcgg tgacgatgtc
     6361 tttcacgtcc aggcgaccaa tttcagaacg cagacggtca gagaacttac gtttcaacag
     6421 cacttccgct tgcgaaatgt cgccaccacc cgttgccagg tagtaacggc tgaaatcgct
     6481 gatgcgccat ttgatgtaag aatcgacgat caggtctttc ttctctttgg tcacaaagcg
     6541 gtcggcctgg ttgtccatgg tctgaatacg tgcgtcgagc attttcaccg tttcaatgaa
     6601 cggtatcttg aaatgcagac ccggctcata aaccagaggt ttgttgtcat cgtcacgcag
     6661 taccttacca aaacgcagcg taataccgcg ctcaccttct ttgacgacaa agacagacat
     6721 gtaaagcact accagcacga tgatgataat cgcgataact gacttacgca tcgttattcc
     6781 ccctgacgct ggtagtcgtt acgctgcgcg ttggcgcggc gttggtccat aatatcgccc
     6841 tgactggtgg acgacgtgtt gcttgctcca ctggttgtgg aggaagaggc tggcggcaga
     6901 cgcagcagat tgctggcacc gttatcgctc ttcgccgcag gggcgttacc gcctttcagc
     6961 atctggtcta acggcagaac catcaggttg ccacctttat cgttaaccag cactttgcgg
     7021 gtgttaccca acactttttc catcgtctcg atatacagac gctcgcgagt aatttccggc
     7081 gcggctttat attccggcag aagtttagca aagcgcgcca cttcaccctg agcttccagg
     7141 atggtctggg ccttgtacgc acgcgcctct tcgaggatac gttgcgcctg accgttcgca
     7201 cgcggctgaa cttcgttggt atacgcttct gcttcacgaa tgtattgctg ttcgttttca
     7261 cgcgcggcaa tcgcatcgtc aaacgccgct tttacttctt ccggcggacg agctgcctgg
     7321 aagttgacgt ccagcagagt gatacccatg tcatacggac ggatagtctc ttccagttcg
     7381 cgctgagtat cgctacgaat cacggtacga ccttccgtca gaatgcggtc catggtgtat
     7441 ttaccgataa ctccacgcag ggcgctgtcg gtagcctgac gcaggctgtc atccgggctg
     7501 gtcacgctat acagatattt ttccggattg gtgacgcggt actgcacgtt catctcaacg
     7561 cgcactacgt tctcgtccga cgtcagcatc acaccagaag cggccagttc acgcacggct
     7621 tccacgttca ccggtttgac ttcgtcgata aacgtcggtt tccagttcag acccggctca
     7681 accagatggc tgaatttacc aaagcgtgtt accacgccgc gttcggcttc tttaatggta
     7741 tagaaaccac tggccgccca gataatgaca atcgctgccg ctgcgatggt aacgacacga
     7801 ccgccaagct gcgggcgcgg gccttgcgat gaactgccac cgccagatcc ggtgccttta
     7861 ccgccgccca gaccaccgag ctttttgctc agtttgcgga agatatcatc taaatcaggt
     7921 ggcccttgat cgcgaccgcc tttgtttcca tttccctcag agttgccgcc aggtttgctg
     7981 cttccccacg ggtcgcggtc ttgtccgtta ttaccgggct gattccacgc catgtttgtg
     8041 ctccatattt gttatgcggt gatccctgtt gtctttcgac gccagaggat atgactccac
     8101 gcttcagacg ctacgccgtt agatcaggta atcgatcaac gccggttctt gtttacagag
     8161 gcgacgccag tcaacgatcg gcatacgaac ttgcagactt acgctgccgt cctcctccat
     8221 ccactctttt tctattgcct gaagctgata aaaacgactt ctcagacgtc cttcctgcgg
     8281 cggcaaacgc aatgtatgct gcgccacctc gccggaaagc cgctccgtca aagcctgaaa
     8341 aagctgtggt atccccgctc cggtctgtgc ggaaagccag acacggatcg gtttgttctc
     8401 ttcgtcccga tcaatacgcg gttcgaaatc ttccagcata tcgatcttgt tcatcaccag
     8461 cagggttggg atctcgtgag cgtcgatctc ttcaagaacc gtattcaccg cttcgatgtt
     8521 ttcttgtaca cgtacatccg ccgcatcaat gacgtgcagc agtaatgtgg cttgccgcgt
     8581 ctcttgtaac gtggctttaa atgctgccac cagatcgtgc ggcaggtggc gaataaaccc
     8641 tacggtatct gcaagtacgg tttcaccgac atccgcgacg tcaatacgcc gcaacgtcgg
     8701 gtcgagggtg gcaaacaact ggtctgccgc gtagacccgc gcttcggtga tgcgattgaa
     8761 aagggtagat ttaccggcgt tggtatatcc caccagcgaa acagtaggaa cgtcggcttt
     8821 gatacgcgat tgccgcccct gctcacgctg cttttcaact ctttctaggc gcgactgtat
     8881 ctgcacgatg cgattacgca acaaacgacg gtcggtttcg agctgggttt cacccggacc
     8941 acgcaaacct atcccgcctt tctgtctttc aaggtgggtc cagccacgca ccaggcgcgt
     9001 agccagatgg cgcagctgcg ccagctcaac ctgcaactta ccctcatggg tacgcgcacg
     9061 ttgggcgaaa atatctaaaa taaggccggt gcggtcgata acacgacact cgcacaaacg
     9121 ctccaggtta cgctcttgcg ccgggctcag ggcatggtca aaaagaacga ccgaagcacc
     9181 cgtcgctttg acagcttccg caatttcaac tgctttacct tcacctacaa aatactttgg
     9241 gtgcggcgct ttacggctac cggtaatcac ctgcaatgct tcgacaccgg cggaagagac
     9301 cagagattca aactcctgga ggtcttccat atctttgtct tgcgtaaaat agatgtgtac
     9361 cagtaccgcc tgctcaccag catcataacg gtcaaacaag cgtataaccc tctaaataga
     9421 tcagcgggga acgcaggatc gctggctccc cgtgtaaaaa aaacagcccg aaaccttatt
     9481 cggtttcttc gctgtcctgt tgcgcggaag tattctgcgc gctgctacca tgatggtagt
     9541 tactgctggt accgccaccg gcgttgttac tgtgatgaga aaccgggcga gacgggacaa
     9601 cagtagaaat cgcgtgcttg taaaccatct ggctgaccgt gtttttcaac aggatcacga
     9661 actgatcaaa agactcgatt tgcccttgca gcttaatacc attcaccaaa taaatagaaa
     9721 ctggaacacg ttcccgacgc agtgcgttca ggaacggatc ttgtaaagat tgccccttag
     9781 ccattctctc ttttccttat atgcttattt gtactttgaa cctttcgatt ctgaaaaaat
     9841 tgcgcacgat acgtctcaat tgtacacatt cagcctgcga tagcaccaac aacctgtaat
     9901 acttcgtcac gcgcctgttc tggtttttca ctgtcaagcc agtgaacccc ttcccaacca
     9961 cgcagccagg ttatctgccg cttcgccaac tgtctcgtgg cgcaaacacc tcgataaacc
    10021 atttcatcgt atgagatttc gccttcaagg taagaccaca tctggcgata acccacgcaa
    10081 cgaatggaag gcaagtccgt atgcaaatct cctcgggcaa aaagcgcccg gacttctgct
    10141 tcaaaacctg aagccaacat ctgatgaaaa cgctgctcaa tgcgttgatg gagcagttca
    10201 cggctcgccg gggcgatggc gaactgatgc acctgatacg gtagagcgtc tcctgacgtt
    10261 tgcgtcagtt ccgttaaagt tttacccgaa atgaaaaaaa cttccagtgc ccgggaaagc
    10321 ctttgtggat catttggatg aatccttgct gccgcaaccg gatctacctc ctgaagttga
    10381 cgatgcaatg actcccaacc ttgctctgcc gcctgttgct caattctggc ccgcacttcc
    10441 gggtctgccg acggtagcgg cgacaaccct tccagcaatg ccttgaaata caacatcgta
    10501 ccgcccacta acagtgggat ccgccccgcc gcggtgatat cggccatttc cgccagcgca
    10561 tcgcggcgaa aatcagcagc cgagtaagcc tgcgacggat cgcgaatatc cagcaatcgg
    10621 tgcggcgcgg cgagtaactc ttcagcgttc ggcttcgccg tcccgatatc catccctttg
    10681 taaataaggg cagaatcaac gcttatcaac tctactggta aaattttacg cagctcaatg
    10741 gctaacgccg ttttaccgga ggccgtcggc cccatcaaaa aaatcgcctt aggcaggctc
    10801 gccttactga tatcactcat ctttcagggc ttttatcgcc ggatgtaaat caacagattg
    10861 taacagacca cccggcggcg ttttcacaag ttgcggacat aaccgttcca cgtccgccag
    10921 cagggttatg gcctgtgcca ttgaccactg cgcatgttcg ctcatcagat ttcgtgcaat
    10981 ccactgcgca atattgccag gttcgaatac ggactgcttc gccaggtagc ctatcagttc
    11041 aggaatcaag atttgtaaat tttgttggcg taagggtaaa ggcacggccc tgatggtcac
    11101 atgctgtgca tctgactgga aatcaatacc caattccgcc agggcagact gcgctttttc
    11161 taatgccgat ttttcttcgc cagaaacttt tagccgcaac ggaataagca gcggctgagc
    11221 gcaaactggc gcttcacccg gcgtcagttg tacctgacgc agccaacgtt ctgccactgg
    11281 caaggctaaa agtgaaatgt tgccgtcgcg ctccagcaac gcacagtcgg aatggacgat
    11341 agtcagtacc cgaccaaaac tctgactgtt cgccgcaagt gcaggttcct gcggttccgg
    11401 cgcttttaat ttttgcatcg gcgcgggcgt ttgcaaaagc tggcgataca cttcaccttg
    11461 ctgtttctgg tagcctggct gcgcattcgg ccagggggca gccggacgac tgcctgatgc
    11521 tggcgcagga gtgtagcgcg gagctaccgg ctcacgagct gccggttctg caaagtgatt
    11581 gcgccccgcc gccacgcggt tttccggaat ggaacgcggt gcaggttggg gttcatcgtc
    11641 cagcggtagc ggcgtttcca gttgctgttg tagcacgctc agcacgccct gatagataaa
    11701 atcatgcacc agacgcgact gatggaaacg cacttcgtgt ttggcggggt gcacgttgac
    11761 gtccacctga tgcggatcga tctccagata cagcacaaat gccggttgct gatcggcccc
    11821 cagtttgtct tcgcaggcct ggcggatcgc gtgattgatc aggcgatcgc gcatcatgcg
    11881 accgttcacg tagcaatact gaatttctgc cagtgcgggc gtggtgtgat ttggatcggc
    11941 cacccagccg cgtagcgtga gatcgccgtg ttgccattca atcgccagcg cttgttcaag
    12001 aaaagcggtg ccgcaaatcg cgcctaagcg ccgttctttt tgcccgcctt ccggcactgc
    12061 gcggtactga cgcacaattt taccgttatg cgacaggttg atcgtgacgt cgaaacgcgc
    12121 cagcgcaatg cggcggatga tctcatcaat gtggttaaat tcggttttct cggtgcgcag
    12181 gaatttgcgc cgcgccgggg tgttgtagaa cagatccagc acctccagcg tcgtccccac
    12241 aggatgcgcc gccggtttta ccgtcacgtt catatcgcgc ccttcggcat aggcctgcca
    12301 ggcttcctgc tgttctgcgg tgcgtgaagt gagcgtcagg cgggaaaccg aactgatact
    12361 cgccagcgcc tcaccgcgaa agcccaggct gataatggct tcgagatcgt ccagagaggc
    12421 gattttactg gtggcatgac gagccagcgc cagcgccagc tcatcttttt tgataccgca
    12481 gccgttatca cgaatgcgga taagtttcgc cccaccgcgt tcgatatcaa tatcgatacg
    12541 cgtcgcacct gcatcgaggc tgttttccac cagttctttg actaccgacg caggtcgctc
    12601 gaccacctca cctgcggcaa tctggttcgc cagttgtggc ggtaagacct gaattggcat
    12661 caatcgtcct tagtttggca gcgtgcgatc tggcgtcgtc accgtactgg cagtttgtgc
    12721 cgttgccccc tgcggcgcag attgcatcgg atgcgcaagg aaataattgc gcaggccttt
    12781 gtaaatggct tctgccagct gttgttggta atcgtcgctc gccagcaaac gttcttcgct
    12841 gttgttgctg ataaaaccgg tttcgaccag tactgacggg atatccggcg aacgcagaac
    12901 gccgagactc gcgtgttctg gtcggcgttt atgtatttcg ccaatgcgtt gcaactgact
    12961 gatcatactg gtcgctacat cataccctac ccgctgggaa tgaccgaact gtaaatccag
    13021 caccgcctgg cttaaatagg ggtcagactg actgttcgcc agcacatcgc ccgccccacc
    13081 cagcagctcc gactgtttct cgtgctgctc cagccagctg gccatttcgc tgttggcgcg
    13141 acggttagag agcacccata cggaagcgcc agtcgcactg cggttcggtg cggcatcagc
    13201 gtgaatcgac acgaggaaat tggcgttttg cttacgtgcc acatcgctgc gccccatcac
    13261 cgagataaag taatccccgt cacgggttaa aacgccttta aacatcggat cgtcattgag
    13321 caaagtgcgc aatttacgcg cgatggcgat ggtgacattt ttctcccgcg taccaccggg
    13381 gccgatagcg ccagggtcct gaccgccgtg tccggcatca atagcgatga taattttatc
    13441 gccagtgtta gccgtcgcgc gcgctgccgg acgcgttacc gtattactgc tgataacacc
    13501 cgtagtgcgg ttactttccg ttttaaacgg attgcgcgcc ggttcgctga cgcgcggtgc
    13561 gacaaccgca ggcgtttcaa cgcgtttcgc aaccacgggc ggtggaggag gcggtggcgg
    13621 cacatcggcg ttaatcgtaa agacgacagt gtaattgctg ccattctgcc gcttcaccgc
    13681 ttcggtttta ccgttttcgg taagatcgac caccagccgt agcgtttgtg catctttagg
    13741 cgttccagag cgaatcgcct tcaccagatt attgccgctg aacaacaacg gcagtccctg
    13801 aatcacgccc gtttgtttga tatcgagcgc cacggtgcgt ttgctttgat ggctaaacgc
    13861 ataatcagga tcgccaataa aactcaacgt tatccgcgcc tgttgattac cgttagaaac
    13921 ctgaatatca gagagcgtcg cggcacccac cggcgtgcac agcagcagca gcgtcgctac
    13981 caaccaattt ctgatgcgat acatcatccc gccacctttc aaaggttaac cggctaaacg
    14041 cgccagcaac aattcacccg cagaggaaac cgcactcacg cgcgcctcac ggccttgtgc
    14101 ctgataatca atgtgtattt cgacatccgg gtcaggaaga acacctgtac cttgttgtgg
    14161 ccactccacc aggcagatgg catcgttggc aaaataatcg cggatcccca taaactccag
    14221 ctcctcggga tcggcaaggc ggtacaaatc aaagtgataa accattaaat tgtcgagcgt
    14281 atagggttcg accagcgtat aagtggggct tttgacatta ccctgatgac ccagagcttg
    14341 taaaaaaccc cggctaaagg tggttttacc tgcgcctaaa tcgccataca gatagattac
    14401 ggttgcgcca tcgcaggctt tcgctacccg ctcgcccagg tctaatgttg cctgctcatc
    14461 agggagcgga attactcgat tcatcatggt ttttatcagt cacttccggg ttaacaatac
    14521 gctgtagcgt ggaaaagaga tcggttgcca gcatcccgcg cgttccaaaa cgcgccgcca
    14581 gtacgtcagc tgccgcaccg tgcgcgacac agcccgcaca ggcggcatca tacggcgaca
    14641 gtttttgccc aagcaatgcg ccaataatac cagagagcac atcgcccatg ccgccgctcg
    14701 ccatgcctgc atttccggca tcaataatgc ctaaagcgtc aggatgggcg gcgaccacgg
    14761 ttccggcacc tttcagcacc gctacgccgc cataacgttg taccagacgt ttggcgcaat
    14821 gtaagcggtc actttcaatt tcagcgacgg aacagcctaa caaccgtgcg gcctcgccag
    14881 gatgcggcgt gatcacgcga ttgtgacgct tatcgggatt gattgccagc aggttcaatg
    14941 catcggcatc ccacaacatc ggtttgcgaa aattctcaac tttttgcagt gcttttttcc
    15001 cccactcttg ctggcccaga ccgggaccaa tcaccaccac atcggcccat tccaggcttt
    15061 cggtaagaga gtccatcgtc agttcatgca ccatcaattc cggtcgtgca gtcagcagcg
    15121 gcgcaatgtt ttcactgcgg gtcagtactc ggactaaacc agcaccagca cgcagcgccg
    15181 cttcccccgt catacgaata gcccccgccg tgccgtgatc gccaccgata atcaccagcc
    15241 gcccgtgatc gcctttatgc gaagtcgggc gacgcggttt cagccagtga gaaagttgtt
    15301 ctgccgaaaa ccgctgaatt ttcgtctcct gacctgccag ccagctatcc agccccagtg
    15361 agtcaaaatg cagttgtcct gtaacatccc gcgcttttcc agtgagcaag cctggtttca
    15421 gcgcaataaa agtgatggtg tgatcggcgt tgatcactgc gcctggcgta gcgccggttt
    15481 cagccagcag gccggaaggg atatcaaccg ccacaatcgg cgcaggatgg gaattagcgt
    15541 ggtcgattaa ctggctaatg gattcgcggg gcgcttgctg caagccggta ccgagcagcg
    15601 catcaacaat cagatctacc gattcgggcc agacaatatt cgaagcatgg atctcgccac
    15661 ccgcgtttaa ccatgcttcg cgtgccagcg cggcctcttc cggcaacggt ttgtcgctct
    15721 cctgggccaa caacgtgacc tcaatgccga ccgctttggc cagtcgcgcg accacgtagc
    15781 catcgccgcc gttattacca tgaccgcaca gcaccagcca gtggcgggcg tcaggatacg
    15841 ccgaacgaca cacctggaat gcggcctcgc cagcgcgaag catcagctca tagagtgtga
    15901 gccccagcac atctgccgcc tcgcgttctc cgcggcggat atcgtcggcg taccagacgg
    15961 tgtgtggtat acttacgggg tttttcttca ttgtatggtc cgtcatgtca gagcccctcg
    16021 atctcaatca gttagcgcaa aaaattaaac agtgggggct ggaactgggc tttcagcagg
    16081 taggtattac cgataccgat ctcagcgagt ccgagcccaa actgcaagca tggctggaca
    16141 aacaatacca cggcgaaatg gactggatgg cacgtcacgg tatgctgcgc gctcgccccc
    16201 atgagttatt accaggtacg ctgcgtgtaa tcagcgtgcg gatgaattac cttcctgcta
    16261 acgccgcatt tgccagcacg ctgaaaaacc ccaaactcgg ctatgttagc cgttatgcgc
    16321 tgggccgtga ctatcacaaa cttctgcgca accgactcaa aaagctgggc gagatgattc
    16381 agcaacattg tgtttcgctg aattttagac cgtttgtcga ttctgcgcct attctcgagc
    16441 gcccgttagc tgaaaaagct gggctcggct ggacaggtaa gcactcactt atcctcaatc
    16501 gcgaggccgg ttctttcttc tttttaggcg aattgctggt cgatattccg ctgcccgtgg
    16561 atcaaccagt cgaggaagga tgcggtaaat gcatagcctg tatgacgatt tgcccgaccg
    16621 gtgccatcgt cgagccatat accgtcgatg ctcgccgctg tatctcttat ctcaccatcg
    16681 aacttgaagg ggcgatcccg gaagagttgc gaccgttaat gggaaaccgt atttacggtt
    16741 gcgatgactg ccagcttatc tgcccgtgga atcgctattc gcaactcact acagaagacg
    16801 atttcagccc gcgtaagccg ctacacgcac aggaactcat tgagttattt gcctggagcg
    16861 aagagaagtt tttaaaagtc acggaaggat cggcgattcg ccgtataggt cacctgcgct
    16921 ggctgcgtaa tatcgccgtg gcattaggca atgcgccctg ggatgaaacg attctggcgg
    16981 cgctggagag tcgcaaaggt gagcacccac ttcttgatga gcacatagcg tgggcgattg
    17041 cgcagcaaat cgagagacga aatgcgtgca tagtcgaagt gcaattgccg aaaaaacagc
    17101 gtctggttcg ggtgattgaa aaagggttac cgcgtgacgc ctgattcatc cacagcttgt
    17161 gaataaaaat aaaaacgcat tgcaattcaa gacgcataaa atcgtcaagt gatcgactcg
    17221 acaaaacaga atcttgattt ttaacttatt tatcaatagg ttaaaaaaat accattcacc
    17281 gtgcgaagtt tctttgaaat taagattaca gcctgaggat gtggataact ctgtttacaa
    17341 gagtttgtca gagacaacaa aaaccagcaa taacgcatcg cgtcgctgtg gatattttat
    17401 tgagagaaga atttggagcg ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg
    17461 tcgtgctcta ccaactgagc tattcccgct tgggtggtct gtgccttaca gcactttcaa
    17521 attttggagc gggaaacgag actcgaactc gcgaccccga ccttggcaag gtcgtgctct
    17581 accaactgag ctattcccgc ttaatcttcg tctttcgaac cgctactgcc ttggctgcct
    17641 tcactcacct tagtcacata cttttgtaag ttcccaagga ttcgttcagt tgccgccttg
    17701 tcgcaattcg aaatactgcg attgggtggt ctgtgctttg cagcactttt caaattttgg
    17761 agcgggaaac gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc tctaccaact
    17821 gagctattcc cgcgtactac ttaattttgc tttcgtaatt ttcgcatttc tgcgtcgtta
    17881 cgggaggcgc attatacgag aaatctttcc ctctgcaacc ccccaaaaaa gttttttttg
    17941 cgtttttatc tcaagtgctg aaattatcag caagacgttc aatttagcgt caaaatcacg
    18001 ccgccgcaag tggattccgg cgcttacgtg accaggaaaa attttacagc ttgataaaat
    18061 gctcgcggta gtaagccagc tccgccaccg attcacggat atcatccatt gcctgatgcg
    18121 tcccctgctt ggtaaaacca tccagaattt ccggcttcca gcggcgcgcc agctctttca
    18181 gggtgctgac atcgagataa cggtagtgga agtaggcttc cagctccggc atgtatttaa
    18241 acaggaaacg acggtcctgt ccgatgctgt taccgcaaat cggcgatttt cccgcaggca
    18301 cccactgttt taagaattcg agcgttgcca gttcagcttc ccgatcgccc atcgtgctcg
    18361 ctttcacgcg ctctaccagc ccgctggcgg tatgggtgcg cacgttccag tcatccatca
    18421 gcgccagctg ttcatcggac tgatgtactg caatggtcgg cccttctgcc agaatattca
    18481 gattggcatc ggtcaccagc gtggcaatct caataatgcg atcgcgctcg ggatccagac
    18541 cggtcatctc aagatcgatc caaatcaggt tgttttcatt ggcactcatg ctattttcca
    18601 cccattcaca gtgactatat tgatccacaa ttgcgtgtat catagatgtt ttgcccatca
    18661 ggggcgacca ggagtcagta cgattgagta aaaataaact ctccaaaggc cagcagcgcc
    18721 gcgtgaacgc caatcaccag cgtcgtctta aaacgtctaa ggagaagccc gactacgacg
    18781 acaatctgtt tggcgagcct gatgaaggta tcgtcatcag ccgctttggt atgcacgctg
    18841 atgtggaatc cgccgatggc gacgttcacc gctgcaatat tcgccgtacc atccgttcgc
    18901 tggtaaccgg cgaccgcgta gtctggcgtc cgggtaaacc ggcggcggaa ggcgtaaatg
    18961 tcaaagggat cgtggaagcg gtgcatgagc gtacctcggt gttgacgcgt ccggatttct
    19021 acgacggcgt aaaacctatt gccgccaaca tcgaccagat tgtcattgtc tccgccattt
    19081 tgccggagct gtcgctcaat attatcgacc gttacctggt ggcctgcgaa accttgcaga
    19141 ttgagccgat tattgtgctc aacaagatag acctgctgga cgacgaaggc atggcgttcg
    19201 tcaacgagca gatggatatc taccgcaata tcggttatcg cgtattgatg gtttccagcc
    19261 atactcagga tgggctaaaa ccgctggaag aggcgttgac cgggcgcatc agcatttttg
    19321 ccgggcagtc tggcgtcggc aaatccagcc tgctgaacgc gttgctgggg ctgcaaaaag
    19381 agatcctgac caacgatgtc tctgacaact cggggcttgg tcagcacacc acgaccgccg
    19441 ctcggctgta tcacttcccg cacggcggtg atgtgattga ttcccctggt gtgcgtgagt
    19501 tcggcctctg gcacctggag ccggaacaaa tcactcaggg ctttgtcgaa ttccatgact
    19561 atttaggtct gtgtaaatat cgcgattgca aacacgatac cgatccgggc tgcgctatcc
    19621 gggaagcggt tgaggaaggg aaaatcgcgg aaacccgttt cgaaaactat caccgtattc
    19681 tggaaagcat ggcgcaggta aaaacgcgta aaaacttttc tgatacggat gactgacaat
    19741 taagctaacc gtcgttagaa tcgtcccctt tttttcagga tccggcatgt aggccgaatc
    19801 aggaacgaca aaacaatggc ctggaggcta ccttgttaaa ttcatttaaa ctttcgctac
    19861 agtacattct gccgaaacta tggcttactc gcctggcggg ttggggcgca agcaagcggg
    19921 caggatggtt gacaaaactg gttatcgatc tgttcgttaa atactacaag gtcgacatga
    19981 aagaggcgca aaagccggac accgccagct accgcacctt taacgaattc tttgtccgtc
    20041 cgctgcgtga cgaagtacgc ccaatcgata ccgatccgaa tgtactggtc atgcctgccg
    20101 atggcgttat cagccagttg ggtaaaatcg aagaagataa aatcctgcaa gccaaaggcc
    20161 acaactacag cctcgaagcc ctgctggcag gcaactatct gatggcggac ctgttccgca
    20221 acggtacgtt tgtgaccact tacctctccc cgcgtgacta ccaccgcgta cacatgccgt
    20281 gcaacggtat tctgcgtgag atgatctacg tgccgggcga tctcttctcc gttaaccatc
    20341 tcacggctca gaacgtgccg aatctgtttg cccgtaacga acgcgtgatt tgccttttcg
    20401 ataccgaatt tggcccaatg gcgcagattc tggtcggagc gacgattgtt ggcagcattg
    20461 agacggtctg ggcgggcacc attacgccgc cgcgcgaagg tatcatcaag cgctggacct
    20521 ggcctgccgg ggaaaacgac ggttctgtgg cactgctgaa aggccaggaa atgggtcgct
    20581 ttaaactcgg ttccaccgtt atcaacctgt ttgcaccggg taaagtgaat ctggttgagc
    20641 aactggaaag cctgtctgtc acgaaaattt gccagccgct ggcagtatct accgaaacct
    20701 ttgttacgcc agacgctgaa cctgccccgc ttcctgctga agagatcgaa gcagaacacg
    20761 acgccagccc attggttgac gacaaaaaag accaggtcta atccatcaaa ggaaacgctg
    20821 acgtgcgcct gattatcact tttctgatgg cctggtgcct cagttggggg gcgtacgccg
    20881 cgacggcccc cgatagcaaa caaatcactc aggaactgga gcaggcaaaa gcggcgaaac
    20941 ccgcacagcc ggaagtcgta gaggcgcttc agtctgcctt aaatgcgctt gaggaacgaa
    21001 aaggttccct tgagcgcatc aaacaatatc agcaagttat cgataattat ccgaaactct
    21061 ccgctactct gcgcgcacaa ttaaacaaca tgcgtgacaa gccgcgcagc gtgtcgccgg
    21121 gaatgtctac cgacgcgctg aatcaggaaa ttctccaggt cagcagccag ttgctggata
    21181 aaagccgtca ggcccagcaa gagcaggagc gcgcccgcga gattgccgat tcgctgaatc
    21241 aactgccgca acagcaaacc gacgcccgcc gtcagttaaa tgagatcgag cgccgcctgg
    21301 gaacgcttac cggcaatact ccgctcaatc aggcacaaaa tttcgcgttg cagtctgact
    21361 ctgcacgtct taaggcgctc gttgatgaac tggagctggc gcagctgtct gccaataacc
    21421 gccaagaatt agcgcgctta cgctcagagc ttgcggaaaa agagagccag caactggatg
    21481 cgtatttgca ggccttacgt aatcagttga atagccaacg tcagcttgag gcggagcggg
    21541 cgctggaaag taccgaatta ctggcagaaa acagtgccga tttgccgaaa gatatcgtcg
    21601 cgcaattcaa aattaaccgc gaactatcgg cagcgctgaa tcaacaggcg cagcggatgg
    21661 atctcgttgc ctcgcaacag cgtcaggctg ccagccagac gttacaggtc cggcaggcgt
    21721 tgaatacgct gcgtgaacag tcgcaatggc tgggatcgtc caatctgctc ggtgaagcgt
    21781 tgcgagcgca ggtggcacgg ctaccagaaa tgccaaaacc acaacagctt gataccgaaa
    21841 tggcgcagtt gcgtgtgcaa cggttacgtt atgaggatct gcttaataaa cagccgctgc
    21901 tacggcaaat ccatcaggcc gacggtcagc cgctgaccgc cgagcaaaac cgtattctgg
    21961 aagcacaact gcgcactcag cgtgagttgc tgaactcatt gttgcagggt ggcgacacgc
    22021 tactattgga actgaccaag ctgaaagtct ccaacgggca actggaggat gcgctgaaag
    22081 aggtgaacga agcaacgcac cgctatctgt tctggacctc tgacgtgcgc ccgatgacca
    22141 tcgcctggcc gctggaaatc gcccaggatc tgcgtcgtct catttcgctg gacaccttca
    22201 gtcagttggg caaagccagt gtgatgatgc tgaccagcaa agagacaatt ttgccgctgt
    22261 ttggcgcgtt gattctggtc ggttgcagta tttactcgcg ccgctatttc acccgttttc
    22321 ttgaacgttc ggcggcgaaa gttggcaaag tgactcagga tcacttctgg ctgacgttgc
    22381 gcactctttt ctggtcgatt ctcgtcgcgt caccgttacc ggtgctgtgg atgacgctgg
    22441 gttacggctt gcgcgaggcg tggccttatc cgctggcggt cgcgattggc gatggtgtaa
    22501 cggccaccgt gccgctgctg tgggtagtga tgatttgcgc cacctttgcc cgcccgaacg
    22561 gcttgtttat cgctcatttt ggctggccgc gcgaacgcgt atctcgtggg atgcgctact
    22621 acctgatgag catcgggctt attgtgccgc tgattatggc gctgatgatg ttcgataacc
    22681 tcgacgaccg tgaattctcc ggttcgctgg gaaggctttg ctttatcctc atttgcggtg
    22741 cgctggcggt ggtcaccctc agcctgaaaa aggccgggat tccgctgtat ctcaacaaag
    22801 aaggcagcgg cgacaacatt accaaccata tgctgtggaa catgatgatt ggcgcgccgt
    22861 tggttgccat tctggcgtcg gctgtgggtt atctggcaac ggcacaggcg ctgttagcga
    22921 ggcttgaaac ctcggttgcc atctggttcc tgctactggt ggtttatcac gttatccgcc
    22981 gctggatgct gatccagcgc cgcaggctgg cgtttgaccg ggcgaagcat cgccgggcag
    23041 agatgttagc gcaacgtgcg cgtggcgaag aggaagcgca tcatcacagt agcccggaag
    23101 gagcaattga agtcgatgaa agcgaagtcg atctcgatgc catcagtgcg caatccttgc
    23161 ggctggtgcg ctcaattttg atgttgatcg ccctgctttc tgtcattgtg ctgtggtcag
    23221 aaatccattc cgctttcggc ttcctcgaaa atatttcgct gtgggatgtc acctccacgg
    23281 tacagggcgt agaaagtctg gagccaatta ccctcggtgc ggtgctgatt gccattctgg
    23341 tgtttatcat caccacgcag ctggtgcgca acctgcccgc gctgctggaa ctggcgattt
    23401 tgcagcacct ggatttaacg ccgggtacgg gctacgccat caccaccatc accaaatatc
    23461 tgctgatgct gattggcggg ctggtcggct tctcgatgat tggtattgag tggtcgaaat
    23521 tgcagtggct ggtcgccgcg ctcggtgttg gtctcggttt tggtttgcag gaaattttcg
    23581 ccaactttat ctctggtctg attatcctgt tcgaaaaacc gattcgcatt ggcgatacgg
    23641 tgacaattcg cgatctcacc ggtagcgtga cgaaaattaa cacccgcgcc accaccatca
    23701 gcgactggga ccgtaaagag ataatcgtgc cgaacaaggc gtttattacc gagcagttta
    23761 tcaactggtc gctctctgac tcggtcacgc gcgtagtgtt gacgattccg gcccctgctg
    23821 atgccaacag tgaagaagtg acggaaatcc tgctcaccgc agcgcgtcgc tgctcgctgg
    23881 tgatcgacaa cccggcaccg gaagtcttcc tggtggatct gcaacaaggg attcagattt
    23941 tcgagctgcg tatttatgcc gctgagatgg gtcaccgtat gccgctacgc catgagatcc
    24001 accagctgat tctggctggc ttccatgccc acggtatcga tatgccattc ccgcccttcc
    24061 agatgcgtct ggaaagcctc aacggtaaac aaacggggag aacgctgacg tctgcgggca
    24121 aaggtcgtca ggcgggaagt ttgtaatgca tgaagctgcc aaatgatgtc aacgtggctc
    24181 atttagcagc ttaacatcat cttcagttat caccggccct ggagagtatc atccatacgc
    24241 attacttcct gacaactcat cgccggagag acgatgagtt gcaggatttc atatgatcag
    24301 tcgagagcta actcaggttt attcgccatg cgactgcaat agttctggta aatcgccatc
    24361 gctaacagcg agaagaagac agggccgcca atcatccaca atgtgctgtc ccagtctccg
    24421 gcttcaacca caggctgaat aatggtgaag acgttcgcaa atgtcaccac cagtacgaca
    24481 accaccatcg caatcattgc cgacatacgc gttttgaaaa tcacaaatgg tctgtcgaga
    24541 tcctgacgtg ctttaaagaa tgggaaagcc agcgcgagga acaggtaagg aagcgtcata
    24601 gacacgttcg ccatcagcgt cagcttgtta aagaacgcgg atgcggtacc gccaccaaac
    24661 gaaaccagca ggatgaagac agtaaccaac ccgcactgca tccacatggc gatagacggc
    24721 atccccatcg cattcaggcg cgtcatcggt tccggccaca acgctttcgg cgtcccctgg
    24781 atgatggctt tcagcggtga atagcaaagc gtaaagaacg caccggtata agcgaggaac
    24841 atcgaaagtc cggtaatacg cgcaaaccat acgcccagcg acaatgacgc ttcaggtgac
    24901 aaatgcagtg cgttacccag cgtcaccccg aggctcttca tcagcacata ggtaatattg
    24961 ccaaggttaa cggaaccatt acttaatacc tgctgccagt ttgtgctgac gccccataaa
    25021 aatattgcca gcgaataacc gattgaaata acaatagcgg caaaaacaat acctttggca
    25081 aagttctttt ctggattttc cgttttatcg accagaccac caaccgcttc aattccgcca
    25141 taggcaaaaa tagcaaatac cacaaacgat agcattgcca gaccggactg ataacccggg
    25201 ttcggtgatg caaggaaatt aatatcctgc gcgaaatgcc caccatttaa taacaaaata
    25261 gtaatgctta ctaacagcaa tactaaattc agacacatta ctgcaatacc gcccaccgca
    25321 gtaatgcggg caattttatt aatccccttt gaagcaacga cggtgaccag aatcatccat
    25381 gctactgcca gcagaccaac cacctgcgta ggctccagtc cggcaatacg ccagtgctgg
    25441 gtcatgtcgc taccatagag gaatgttgag aacggtaccc aaactttcgc ggaggtactc
    25501 accatccaga tgatataaga ggaaaaccac ataaacgtac caatgaaggc aaaacgtggt
    25561 ccgacactat tattcatcca ggaatagata ccaccttctt ctttgcgata agcagctccc
    25621 atttcagcca tcattaa
//
LOCUS       DAESHI010000052        25452 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.052, whole genome shotgun sequence.
ACCESSION   DAESHI010000052 DAESHI010000000
VERSION     DAESHI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25452
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.052"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            126..449
                     /gene="soxS"
                     /locus_tag="KW011_002243"
     CDS             126..449
                     /gene="soxS"
                     /locus_tag="KW011_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HBH7660562.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(452..2038)
                     /gene="pdeC"
                     /locus_tag="KW011_002244"
     CDS             complement(452..2038)
                     /gene="pdeC"
                     /locus_tag="KW011_002244"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HBH7660563.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            2468..2748
                     /locus_tag="KW011_002245"
                     /pseudo
     CDS             2468..2748
                     /locus_tag="KW011_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(2847..3383)
                     /gene="ssb1"
                     /locus_tag="KW011_002246"
     CDS             complement(2847..3383)
                     /gene="ssb1"
                     /locus_tag="KW011_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HBH7660564.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            3637..6459
                     /gene="uvrA"
                     /locus_tag="KW011_002247"
     CDS             3637..6459
                     /gene="uvrA"
                     /locus_tag="KW011_002247"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBH7660565.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(6494..6850)
                     /locus_tag="KW011_002248"
     CDS             complement(6494..6850)
                     /locus_tag="KW011_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBH7660566.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(6854..7270)
                     /locus_tag="KW011_002249"
     CDS             complement(6854..7270)
                     /locus_tag="KW011_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HBH7660567.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(7381..8094)
                     /gene="aphA"
                     /locus_tag="KW011_002250"
     CDS             complement(7381..8094)
                     /gene="aphA"
                     /locus_tag="KW011_002250"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HBH7660568.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            8496..8736
                     /locus_tag="KW011_002251"
                     /pseudo
     CDS             8496..8736
                     /locus_tag="KW011_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9240..10433)
                     /gene="tyrB"
                     /locus_tag="KW011_002252"
     CDS             complement(9240..10433)
                     /gene="tyrB"
                     /locus_tag="KW011_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HBH7660569.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(10686..11765)
                     /gene="alr"
                     /locus_tag="KW011_002253"
     CDS             complement(10686..11765)
                     /gene="alr"
                     /locus_tag="KW011_002253"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBH7660570.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(11818..13233)
                     /gene="dnaB"
                     /locus_tag="KW011_002254"
     CDS             complement(11818..13233)
                     /gene="dnaB"
                     /locus_tag="KW011_002254"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBH7660571.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            13316..14299
                     /locus_tag="KW011_002255"
     CDS             13316..14299
                     /locus_tag="KW011_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HBH7660572.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(14465..14707)
                     /gene="pspG"
                     /locus_tag="KW011_002256"
     CDS             complement(14465..14707)
                     /gene="pspG"
                     /locus_tag="KW011_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HBH7660573.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(14841..15878)
                     /gene="dusA"
                     /locus_tag="KW011_002257"
     CDS             complement(14841..15878)
                     /gene="dusA"
                     /locus_tag="KW011_002257"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HBH7660574.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(16241..17226)
                     /locus_tag="KW011_002258"
                     /pseudo
     CDS             complement(16241..17226)
                     /locus_tag="KW011_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            17544..18059
                     /gene="zur"
                     /locus_tag="KW011_002259"
     CDS             17544..18059
                     /gene="zur"
                     /locus_tag="KW011_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HBH7660575.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(18101..18310)
                     /locus_tag="KW011_002260"
     CDS             complement(18101..18310)
                     /locus_tag="KW011_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBH7660576.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(18426..19751)
                     /gene="dinF"
                     /locus_tag="KW011_002261"
     CDS             complement(18426..19751)
                     /gene="dinF"
                     /locus_tag="KW011_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HBH7660577.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(19824..20432)
                     /gene="lexA"
                     /locus_tag="KW011_002262"
     CDS             complement(19824..20432)
                     /gene="lexA"
                     /locus_tag="KW011_002262"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HBH7660578.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(20542..20910)
                     /gene="dgkA"
                     /locus_tag="KW011_002263"
     CDS             complement(20542..20910)
                     /gene="dgkA"
                     /locus_tag="KW011_002263"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBH7660579.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            21081..23504
                     /gene="plsB"
                     /locus_tag="KW011_002264"
     CDS             21081..23504
                     /gene="plsB"
                     /locus_tag="KW011_002264"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HBH7660580.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(23659..24531)
                     /gene="ubiA"
                     /locus_tag="KW011_002265"
     CDS             complement(23659..24531)
                     /gene="ubiA"
                     /locus_tag="KW011_002265"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HBH7660581.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(24544..25041)
                     /gene="ubiC"
                     /locus_tag="KW011_002266"
     CDS             complement(24544..25041)
                     /gene="ubiC"
                     /locus_tag="KW011_002266"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HBH7660582.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(25264..>25452)
                     /locus_tag="KW011_002267"
     CDS             complement(25264..>25452)
                     /locus_tag="KW011_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660583.1"
                     /translation="DMLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYAL
                     FPIFTASWRATRIKNIGI"
BASE COUNT         6586 a         6545 c         6093 g         6228 t
ORIGIN      
        1 gggttagcag cgctttaatg cggggtaatt tcttttccat aaatcgcttt acctcaagtt
       61 aacttgagga attatactcc ccaacagatg aattaacgaa ctgaacactg aaaagaggca
      121 gatttatgtc ccatcagaaa attattcagg atcttatcgc atggattgac gagcatattg
      181 accagccgct taacattgat gtagtcgcaa aaaaatcagg ctattcaaag tggtacttgc
      241 aacgaatgtt ccgcacggtg acgcatcaga cgcttggcga ttacattcgc caacgtcgcc
      301 tgttactggc cgccgttgag ttgcgcacca ccgagcgtcc gatttttgat atcgcaatgg
      361 acctgggtta tgtctcgcag cagaccttct cccgcgtttt ccgtcggcag tttgatcgca
      421 ctcccagcga ttatcgccac cgcctgtagt tttattgccc gcgcgttaac tcccgcgcgg
      481 gtaattgctc catccattgc ataaacacct gcggcggcat cgccttcgca aaaaaccatc
      541 cctggcaata gcgcacgccg cgtttgcgca gccagttaac ctgctcctca gtttcgacgc
      601 cttcagcgat cgtttttaac cccaggctgt gcgccagctc gatgatgtgt tccgcaatca
      661 aatgactggt tttgtgggtg gtcagcgttt caacaaacga tttatcgatt ttcaaaatat
      721 cgacattcaa tgatttaagg ttatgcaagt tagagtagcc aataccaaaa tcatcaattg
      781 ccacttcgta accagcctgg cggaaagcca gaataatcgg cgtcattttt tcgacatcaa
      841 gaaatgcatg ctcagtcact tcaaatttaa tttgctgcgg acgcaccgcg tattgctctg
      901 ttttctgatt gattcgcgct atcaaccgtg acgtatgaaa atcggaggcc gacaggttaa
      961 tagaaacata gcgatcggca tgtgttgcca agtacgcgcc cagatcgcgg aagacattat
     1021 caataacata atcagttatc tgttctatca tcccctcctt ttctgccagc ggaataaact
     1081 ctgccggatt cattatttgc ccctgctcac caagccaacg taacaacgct tcagcgccga
     1141 tacatttttc tgttttgata tcgattattg gctggtaata aagacaaagt tgatgttttt
     1201 cgagggcgcg ttgcaattta cgtttgggag ataaatagtt ttgtcgaata cgtagccaga
     1261 gtaatagcag aacaagactc cccaaaatac cggcgggcaa cgcaaaaata agatgattat
     1321 aaaaatgggt tataagacgt tgatatgaag tcgcaacaat ggctgcaatt gggcgttttg
     1381 tcgaataaac tgtcgcatat aaatagccat ttctttgtac ggttaaatcc ttcaaatgaa
     1441 tcagcggaga aaacgttgct gccgaggcct ctttgcttaa cgagaaaaag gttttcgtca
     1501 ccgtatcata cacaccccat tgcaatgtcg ggtcatcaga catcacttca ctccagaaga
     1561 gagggttgat aaccgccaca taatttcccc gctgcatata ggtcatttta tagccagaga
     1621 aaaaaggcgt atcgcggtaa taatagatag aaacgttagg ttcacgctta taatcggccg
     1681 gtgcaatcgt atagccgttt acaggcgcta tcagcgatga gcataaaaaa tggttatcac
     1741 gggcatagat caattcatta atataaagat agccacgaat aatattcaac attcgctttt
     1801 gatgggctgg agtgcaaact tgcccctgat agcgttcagc tgcatcgctc actaaatctg
     1861 cctggcgaat gaccagctcg gatttatcca gtgccagttg agcaaaggtt cggagctgat
     1921 tattcacttc tgattttgcc caaaggaagg caatccatag cgaaagaatg atgggaaaga
     1981 gaactaaaaa gataatgccc ggcaacgcca gtaattggtg tcgtgcacga tgactcattg
     2041 ctatcaattt cctgcctgtt tatacccggc cattgcaagg tgaaaaccct gggtaccgac
     2101 gtctttagat aaatcttacg gtataaatag cacttttgcc tgatttgaaa ttgatacctt
     2161 tctaatcgtc acctggatga aaataattca ttgatattca aaattaattt cctacaggaa
     2221 tgcagaggcg gcgggaagat atgcccttaa tgccactgaa aaaccacata atctgtacag
     2281 caataaaagt cacggcctaa tatggctaac aaacaatcaa ttgtgatata gctcacaaaa
     2341 ttaatgaaac aaacagagtg tttcattttt gtgttctgca agccccgcga tagccagagt
     2401 gtaccacgcc tcccgtgaac aacgccgcgc tgtccagggc atcggctctt ttcagaggaa
     2461 ttgtttgatg gctaccctca ccactggcgt ggttcttctt cgctggcagc ttcttagtgc
     2521 cgtaatgatg tttctggcca gcacatcaac atccgttttc gtcggtctga ttatgtcggg
     2581 cttgcggtga tcagcagcgg tctgggcgtt gtttccgcct gttggttcgc aatggggttg
     2641 cttggcatca caatggcgga catcaccgct atctggcaca acatcgagtc ggtgatgata
     2701 gaagagatga atcagacacc gccacaatgg ccaatgattt tgacctgata cgtagaagcc
     2761 tccataacgg aggcttcttt tttatggctg gcagatgctt taatcatcca ccttaaaaca
     2821 atataaccta ttgttttaat gacaaatcag aacggaatgt catcatcaaa gtccatcggc
     2881 ggctcgttag acggcgctgc cggagcggac tgctgcgggc gagactgcgc gccgccgctg
     2941 aactgattgc caccctgcgg ctgctgaggc tgaccccaac cgccctgcgg ctgaccacca
     3001 ccgatattac cacctgccgg agcgccacca ccctgacgac cacccagcat ctgcatggtg
     3061 ccgccaacgt tcaccacgac ttctgtggtg tagcgatcct gaccggattg atcggtccat
     3121 ttacgggtac gcagctgacc ttcgatataa acctgagaac ctttacgcag atattcgctg
     3181 gccacttctg ccagtttgcc gaacagcaca acgcggtgcc attcagtctg ttctttcatc
     3241 tcgccggtcg ctttatcacg ccaggattcg gaagtagcca gcgtaatgtt ggcaactgcg
     3301 ccaccatttg gcatgtagcg tacttccggg tcctgaccca gattaccaac gagaataacc
     3361 ttgtttacgc ctctgctggc catgttcgtg tctcctgaaa aaaatcgttc tgaataagtg
     3421 taaacgcgcg attgtaccat taccaatagc gcttttacta tgttgtgacc tcggttccgg
     3481 gaaacaaacc tggccagaca ttgttacaca acactccgga taatgcattc caatactgta
     3541 tattcattca ggtcaatttg tgtcataatt aaccgtttgt gatcgccggt agcaccatgc
     3601 caccgggcaa aaaagcgttt aatccgggaa aggtgaatgg ataagatcga agttcggggc
     3661 gcccgcaccc ataatctcaa aaacatcaac ctcgttatcc cccgcgacaa gctcattgtc
     3721 gtgaccgggc tttcgggttc tggcaaatcc tcgctcgctt tcgacacctt atatgccgaa
     3781 gggcagcgcc gttacgttga atccctttcc gcctacgcgc ggcagtttct gtcactgatg
     3841 gaaaagccgg acgtcgatca tattgagggg ctttctcctg ccatctcaat tgagcagaaa
     3901 tcgacgtctc ataacccgcg ttctacggtg gggacaatca ccgaaatcca cgactatttg
     3961 cgtttgttgt tcgcccgcgt cggcgaaccg cgctgtccgg accacgacgt cccgctggcg
     4021 gcgcaaaccg tcagccagat ggtggataac gtgctgtcgc agccggaagg caagcgtctg
     4081 atgctgctcg cgccaatcat taaagagcgc aaaggcgaac acaccaaaac gctggagaac
     4141 ctggcaagcc agggttacat ccgtgctcgt attgatggcg aagtctgcga tctttccgat
     4201 ccgccgaaac tggaactgca aaagaaacac actattgaag tggtggttga tcgcttcaag
     4261 gtgcgtgacg atcttaccca acgtcttgcg gagtcgtttg aaaccgcgct ggagctttcc
     4321 ggtggtacag cggtagtggc ggatatggac gacccgaaag cggaagagct gctgttctcc
     4381 gccaacttcg cctgcccaat ttgcggctac agtatgcgtg aactggagcc gcgactgttt
     4441 tcgtttaaca acccggcagg tgcctgcccg acctgtgacg gccttggcgt acagcaatat
     4501 ttcgatcctg accgcgtgat ccaaaatccc gagctgtcac tggctggcgg tgcgatccgt
     4561 ggctgggatc gccgcaactt ctattatttc cagatgctga aatcgctggc agatcactat
     4621 aagttcgacg tcgaagcgcc gtggggcagc ctgagcgcga acgtgcataa agtggtgttg
     4681 tacggttctg gcaaagaaaa cattgaattc aaatacatga acgatcgtgg cgatacctcc
     4741 attcgtcgtc atccgttcga aggcgtgctg cacaatatgg agcgccgtta taaagagacg
     4801 gaatccagtg cggtacgtga agaattagcc aagtttatca gcaatcgccc atgcgccagc
     4861 tgcgaaggaa cgcgtctgcg tcgggaagcg cgccacgtgt atgtcgagaa tacgccgctg
     4921 cctgctatct ccgacatgag catcggtcat gcgatggaat tcttcaacaa tctcaaactc
     4981 gcaggtcagc gggcgaagat tgcagaaaaa atccttaaag agatcggcga tcgtctgaaa
     5041 ttcctcgtta acgtcggcct gaattacctg acactttccc gctcggcaga aacactttcc
     5101 ggcggtgaag cccagcgtat ccgtctggcg agccagattg gtgcgggcct ggttggcgtt
     5161 atgtatgtac tggacgagcc gtctatcggc ctgcaccagc gcgataacga gcgcctgttg
     5221 ggtacgctta tccatctgcg cgatctcggt aataccgtga ttgtggtgga gcatgacgaa
     5281 gacgcaattc gcgccgctga ccatgtgatc gatatcggtc ctggtgcggg tgttcacggc
     5341 ggtgaagtgg tcgcagaagg tccgctggaa gcgattatgg cggtgcctga atcgttgacc
     5401 gggcagtaca tgagcggtaa acgcaagatt gaagtgccga agaaacgcgt tccggcgaat
     5461 ccagaaaaag tgctgaagct gacaggtgca cgcggcaaca acctgaagga cgtgacgctc
     5521 acgctgccag tcggtctgtt tacctgcatc acaggggttt caggttccgg taaatcgacg
     5581 ctgattaacg acacactgtt cccgattgcc caacgccagt tgaatggtgc gaccatcgcc
     5641 gaaccggcac cgtatcgcga tattcagggg ctggagcatt tcgataaagt gatcgatatc
     5701 gaccaaagcc caattggtcg tactccacgt tctaacccgg cgacctatac cggcgtgttt
     5761 acacctgtgc gcgaactgtt tgcgggcgta ccggaatccc gtgcgcgtgg ttatacgcca
     5821 ggacgtttca gctttaacgt ccgtggcggg cgctgcgaag cctgtcaggg cgacggtgtg
     5881 atcaaagtgg agatgcactt cctgccggat atctacgtgc cgtgcgatca gtgcaaaggt
     5941 aaacgctata accgtgaaac gctggaaatt aagtacaaag gcaaaaccat ccacgaagtg
     6001 ctggatatga ccatcgaaga ggcgcgtgag ttctttgatg cggtgccagc tctggcgcgt
     6061 aagctgcaaa cgttgatgga cgttggtctg acgtacattc gcctggggca gtccgcaacc
     6121 acactttctg gtggtgaagc ccagcgcgtg aagctggcgc gtgagctgtc aaaacgcggc
     6181 accgggcaga cgctgtatat tctcgacgag ccgaccaccg gtctgcactt cgccgatatt
     6241 cagcaactcc tcgacgtgct gcataaactg cgcgatcagg gcaataccat tgtggtgatt
     6301 gagcacaatc tcgacgtgat caaaaccgct gactggattg tcgacctggg accagaaggc
     6361 ggcagtggcg gcggcgaaat cctcgtctcc ggtacgccag aaaccgtcgc ggagtgcgaa
     6421 gcttcgcata cggcacgctt cctcaagccg atgctgtaat cgttaaggcc gctttctgag
     6481 cggccttttc ctttcagagt tgtaccagca atttacgctt ttcttccggt agtaaattca
     6541 ctgcctgctg ataagacgca tccaccagat aatagatttg cgaatccggc agcgaaccgt
     6601 cgagatacac ggtgctccag tgcgctttat tcagatggcg gctaggacgc acatcgctgt
     6661 gctgctgacg tagcagctcc gccagctccg ggctggtttt cagcgaaaca gctgggcgat
     6721 tttctacttc tttcaccatc gcaaacagta catcttccac tttgatctgc gtcgctttcc
     6781 agtcgttatg cacgctctgt tctgcgcctg gttttgccat gcaatattgt agcaactccg
     6841 aaatggtcat tttttactcc ccttgtagtg tcgcgatgat gcgacgcgat ccgccgtgga
     6901 tgcgatgttc ccccagccag atgccttgcc aggtgccggt ctgaatacgc cctttatgta
     6961 acggcaatac aagcgatgtt cccagcattg aggatttgat atgagaaggc atatcgtctg
     7021 ctccctcata gtcatgctca taatttccgt tgtcgggaac ggtgcggagg aaaaaacgct
     7081 ccatgtcgtg gcgtacggtg ggatcgcagt tctcattaag tgtcagagag gcggaggtat
     7141 gttgcagcaa cagatgcagt aagccgatgt taacgcgcgg catatcagcc agctgattca
     7201 gaatttcatc cgttaccaga tgaaacccac gagatttggc gctaagcgtg agcgtctttt
     7261 gataccacat gtgctgctcc ttgataaaac tctcttaatc agtttgcagc aagacagcga
     7321 aaggataaag gtgtgattaa aaaaacagca ttcgggagag catcacgctc tcccgctctg
     7381 tcagtattct gaattgacga tcacctcttc accaaacgca cccgcttgtg gcaagggttt
     7441 gtaggtagag ttggaggcgc gcagaatgcg gataccacga gcgccgacat cgcgtgcggc
     7501 ggtaatatcg ttatcggaat cgccataaaa aattcggata tttttatcct gcagccattg
     7561 cgattttgta ttttgccctg gtttatcgcc cgcaaagatt accggattca tgttggtggc
     7621 aggaatatga aaattatccg ccagcgtttt tgaaaccgtt tctgttttcg tcgggctacg
     7681 accagtcaca aagaagatcg cgtcaccgcg gcgtacatgc atatcaatca gctggcgagc
     7741 gacctctttt ggaatgctga attcatccca gccattattc attttttccc agaacacagg
     7801 atttttcaga taatcttcgc tttctggcga gaaggttttt ttgccgcgcc agaaccccgg
     7861 actggaaaaa agtaccgtgt catcgatatc aaaccccacc gccattggcg gacgccctgc
     7921 gaggctattt tcaatttgtg cgaccgaaac ccaatgaatt ggtgcctgtt cagcaagcct
     7981 ggcaacgtta gtcccagggt taagcggtga aggagatgag gccagggcaa cagcggaact
     8041 gtttagagcg aacaataagc aaacggcact gattgcctgt gtgatcttgc gcatattttt
     8101 ccctaaatag tcagtatgtt gaaacttttt attgtgagat ttgttgcaaa aaccatctga
     8161 ccataacgcc agctgccggg gaaaggaagg attatctgcg gtttttgtga tgaaaagcag
     8221 gatgattata tttaacccag taaatacgtt cctgtctttt tttgtataaa aaatgtttat
     8281 tactcgatgt aaggatacat taattttacg tttattatga tgaatcctct gcaaatgagc
     8341 gaaatttgaa agaacattta tctgctgtac gatttaatga acttaatgat acagcattac
     8401 aaaaacaatc gaagtttata aagatgattc ctgattgacc agaaccctat cctacaatgg
     8461 tgactaattt tattactccc gatggagatg atgatatgaa tatatcatat gtgaatagca
     8521 acaaaacgac atctttacct gttgagctag atgcactaaa taataaagac atctcttatg
     8581 ccaaagattt ttcttatgcc aaagattttt cttatgctaa agatttcttt ttatacatag
     8641 aaacacagtt aaaaattgca aaagattttt gtagacctgg agaagaagta tcaagttcta
     8701 ttgcaagtaa agtttttcac gcatttatcg atttagttaa caaaatcagg ggtaagaaag
     8761 actttatgta tatttgcacg ctttgctgtt ttgccgagga ggttaaaggt gattattctc
     8821 attacaggac ctttttattt gatattggta atcaatataa ggttaaactt acacaaagcg
     8881 gaaaaaaaga gttctcttta acattagaat ttaacgatac tataattgaa tctcagaaag
     8941 tcacaggcaa taaggcaaag tatattcttg aggatataga aatattctat cgtaacaaac
     9001 ccgatactta ttattaaatt taataatggt tagatgtgca gaaattatta ctccacacga
     9061 aaaaataggg aaaggaattc cagataattg ggataaactt aacaataaaa gaactgacaa
     9121 cgttttatta tctctcacgt agaacaacgg catcaaaaaa atcatctaca aagaaatagt
     9181 gaaagcgacg acagtttaat ttcactgcag gctgggtaac tccagcctgc tttcctgcat
     9241 tacatcaccg cagcaaacgc ctttgccaca cgttgtacat ttgccgtatt taacccggcg
     9301 acacacatgc gaccgctggc gatgagatag acaccaaatt cttcacgtag tcggtcaacc
     9361 tgagcggcac ttaaaccggt ataactgaac atgccgcgct gattaagcag ataatcgaaa
     9421 ttgcgttctg gcatctctgt gcttaatacc ttcaccaatt cctgacgcat tgccagaatg
     9481 cgagtacgca tctcttctac ttccgccagc cagctggctt tcaatgcctc gtcattcagc
     9541 actgcagcca ccacctgcgc accaaaattc ggcgggctgg agtagttgcg gcgaactgtt
     9601 gctttcaatt gccccagtac gcggccagcg gcttcggcat cttcacacag aacagaaagt
     9661 ccgccgacgc gctcgccgta aagggagaaa attttcgaga acgaattgct caccagagcg
     9721 ggtaatccag cgctggcaat ggcgcgaata gcgtaggcat cctcttccat accggcacta
     9781 aatccttgat aggcaatatc gaggaatggg ataagctcgc gggctttgag aatttcaatc
     9841 accgcatccc actgatcatt agtgagatcg gcacccgttg ggttgtggca acatggatgc
     9901 agcaacacaa tactgcgggc aggtaatgtt ttcagcgtcg ccaacaggtc attaaagcgc
     9961 acgctgttag tcgcttcgtc ataccagggg taagtactca cttcgaatcc agccccggcg
    10021 aatattgcta cgtggttttc ccaggtagga tcgctgaccc agacgcctga ttccgggaag
    10081 tagcgtttca ggaaatccgc gcccactttc aatgcccctg agccgccaag ggtttgaatg
    10141 gttgctacgc gctgttgttg cagtaccgga tggtccgcac caaacagcag cggcgcaatg
    10201 gcatggcgat agctgttaag cccttccatc ggtaaataaa gcgaagcgcc atggggctgc
    10261 gcattcaggc gcgcttccgc ctccgccacg gctttcagtt gtggaataat tccgtcttcg
    10321 ttgtagtaca gaccgatact taaattcact ttgtcgctgc gagggtcttc tttaaaacgc
    10381 tccataagcg taagaatcgg gtcgccagcg taggcgtcaa ctttttgaaa cacgcgatgg
    10441 ttctccaggt ttacgggcag gtggttaaaa cacaataaac cggaagaagg cgaagatcga
    10501 gtggatgttc aggagcgaac ggcaattagc aacagagtga gactcatgac aaacgaacat
    10561 ccgccagagg cacgaccttt ataagagcat gaaagaacat caacttattg aatttttagg
    10621 attttattgg ccggataagg cattcacgcc gcatccggca cagacaatca aatattacag
    10681 aacgattaat ccacgtattt catcgcgacc cttgaagtca ggcgcgtaat aagttcgtaa
    10741 gcgctcactt ttgtcatttc agcgatacgt tctacgggca aaccttcgcc ccataaaatg
    10801 accggatccc cggctttgtc ctgcgcctgt ggacctaagt ctacgcagat catatccatc
    10861 gccacgcgtc cgacaatcgg tacttcgcga ccgttcacca gcactggcgt accggatgga
    10921 gcggcgcgcg gataaccatc gccatagccc atcgcgacta cgccaagacg ggtatcacgt
    10981 tcgcttaccc aggttccacc ataaccaaca ggctctccgg ctttatgctc acgcacggca
    11041 atcaggctgg aggttagtga catcactggc tgacagccaa aatcggcacc gatggagcga
    11101 tcttccagcg gcgagacgcc ataaagaatg atgcccgggc gcacccagtc aaaatgcgat
    11161 tgtggccaca gcagaatgcc acccgacgcg gcaatggaac gttgaccagg tttgccttcg
    11221 caaaaggtat taaagatagc gagttgtttc tcggttgcgc cacattttgg ttcatccgcg
    11281 cgcgcaaaat ggctgacgat attcaccggc tgacgaacgt ttttgcactg tgtcaggcga
    11341 tgataaaacg cctcagcctg ttccggcctt acgcccagac ggtgcatacc ggtatcgagt
    11401 ttcatccaga cggtaaccgg ctcgtccagg ctagcctctt ccagcgcagc cagctgttct
    11461 tcgttatgca cggcggtatg aaaatgttgc gcagaaatcg tcggcagatc tctggcatca
    11521 aaaaagcctt cgagtaacag tacaggtttg gtgattcccc ccgcacgcag tcgcagagct
    11581 tcttcgagac gggctacgcc aaaggcgtca gcatcgggga gcgttcgcgc ggtctcaaga
    11641 agaccgtgac cataagcgtt cgctttcacc accgcaacca ttttactggc aggggccagt
    11701 tcacgaagac gttgcaggtt gtgtcgcaga gcgcggcggt taatcacaac agttgccgct
    11761 tgcatttgtg ttccttgata agtgtttgct ttaattacct aattcataaa ataattatta
    11821 ttcgtcgtcg tactgcggcc ccgcatagtt gtcgaagcgc gaccattgac cgttaaaggt
    11881 caggcgtacc gtcccgattg ggccgttacg ttgtttaccg ataataattt ccgcgatgcc
    11941 ttttaaatca ctgttttcgt gatacacctc atcacgatag ataaacataa tcaagtccgc
    12001 atcctgctcg atagagccag attcacgcag gtcggagttg accgggcgtt tgtcggcacg
    12061 ttgttccaga gaacggttca actgggacag cgccaccacc ggcacgttca gttctttcgc
    12121 cagtgctttc agcgagcgag agatttctgc aatttccagc gtacggttat cggaaagcgc
    12181 cggtacgcgc atcagttgca ggtagtcgat catgataagc ccgatgccgc cgtgttcacg
    12241 ggcaatacgg cgtgcgcggg aacgcacttc cgttggcgtc aggccggagg agtcatcgat
    12301 atagatattg cgtttttcga gcaaaatacc catggtgccg gaaatgcgcg cccagtcttc
    12361 atcatcgagc tgcccggtac ggattttagt ctggtcaacg cgcgacagcg acgccagaga
    12421 acgcatcata atctgttctg aaggcatctc cagcgagaag ataagtactg gtttatcctg
    12481 caacatcgcc gcgttttcga cgaggttcat cgcaaatgtt gttttaccca tcgacggacg
    12541 cgcggcgacg atgatcaaat ccgacggctg caagccagcg gtttttttgt tgagatcgtc
    12601 ataaccggtg tttactccgg taacgccatc gtgtggctgc tgaaacaact gctcaatacg
    12661 cgccacggtt gcgtcgagca catcggcgat gttcttcggc ccttcgtctt tgtttgcacg
    12721 actttcggca attttaaaga cgcgggattc agcaaggtcc agcagatctt cgctggtacg
    12781 cccctgcgga tcaaaaccgg cttcagcaat ctcattcgca accgagatca tctcacggac
    12841 aacggcacgt tcacgcacga tgtcagcata agcactgatg ttcgccgcac ttggcgtatt
    12901 ttttgacagc tctgccagat aagcaaaacc accgacgcta tcgagttgcc cctggcgttc
    12961 cagcgattcc gcaagggtaa tcagatcgat aggactaccg ctttcctgca aacgcgccat
    13021 ttcagtaaag atatggcggt gtgggcgggt gtaaaaatcg tctgccacca cacgctcggc
    13081 tacatcgtcc cagcgttcgt tatccagcat taaaccgccc aacaccgact gctccgcttc
    13141 gatcgagtgc ggaggcactt tcagcccggc aacttgtgga tcgcgttcgc ggggttcagc
    13201 ctgctgtttg ttgaagggtt tatttcctgc catagtgaat ggagttaccg agataaagaa
    13261 tgggtcgaaa ctttaccata tgaagcagac cctgacgata cgttctggag gacacatggc
    13321 aacacgaatt gaatttcaca agcacggtgg cccggaagta cttcaagccg tagagttcac
    13381 tcctgccgat ccggcagaga atgaaatcca ggtcgaaaat aaagccatcg gcatcaattt
    13441 tatcgacacg tatatccgca gcggccttta cccgccgcca tcactaccca gcggattagg
    13501 taccgaagcg gcaggcatcg tgagtaaagt cggcagtggt gtaaagcata ttaaggcagg
    13561 cgatcgtgta gtctatgcgc agtcggcgtt aggcgcttac agctctgtgc ataacattaa
    13621 tgcggataaa gcggcgattc tgcctgcggc aatttctttt gagcaagctg cggcatcctt
    13681 cctgaaaggc ttaacggttt attatctgct gcgcaaaacc tatgaaatta aacccgatga
    13741 acagttcctg ttccacgcag cggctggcgg cgttggctta attgcctgcc agtgggcaaa
    13801 agccctgggc gcgaaactta tcggcaccgt aggaaccgcg caaaaagcgc agagcgcgct
    13861 aaaagcgggc gcgtggcagg ttattaacta tcgtgaagag gatctggtcg agcggttaaa
    13921 agagatcacc ggcggtaaga aagtgcgcgt ggtgtacgat tccgtgggca gagacacctg
    13981 ggaacggtcg ctggattgcc tgcaacgccg cggcttaatg gtcagttttg gcaactcatc
    14041 aggtgcggtt accggtgtga acttaggcat tctcaatcaa aaaggctcgt tgtatgtgac
    14101 acgcccttcc ctgcaaggct atatcaccac gcgggaggaa ttaaccgagg ccagtaatga
    14161 actgttctct ttgattgcca gcggtgtgat taaggtcgat gtcgccgagc agcagaaata
    14221 tccgctgaag gatgcgcagc gtgcgcatga gattctggaa agccgggcga cgcaaggttc
    14281 cagcctgttg attccataaa agaaataggg cttccacctg ggaagccctt tctttttata
    14341 gttcggctgt atgtagggta cagcacgatg aatctgttag aggcgcaata gtgacagatt
    14401 tgattatcaa ttcctatttt gttctaagga taaaacctta ggttgtgatc atccgcacaa
    14461 tcccttagta acgccagcgg tcataacgct gatatttcgg cacttttggt gctttaatcg
    14521 ccttaataac ccacaccacc gcaatcgcca gtagtaacca cggcagcaac ttaatcatca
    14581 atgccagcat accgccgagg aacataatgg ccgtcgccac aaccagcgcg gcgataatgc
    14641 ccagcaacga aacgccggtg accatcagca tgacaaaaaa gccaatcaca aaaagtagtt
    14701 ccagcatgat gctctcccaa atatgaaatc tcttgctggc attacaagaa tcatgccaaa
    14761 aataatctat tgatttaaca gcaaaacgcc ccgcgacggt gcgcagggcg tggtgaattt
    14821 gactactttt tggtgaaaag ttaacgctta tccgccacca gtttgagcgc gtgttccagc
    14881 acattaatgt ctgcacccgc tttatgggca ttttcactta aataacgccg ccactgccgc
    14941 gcgccaggaa taccctggaa caagcccaac atatgccggg taatatggcc gagatacgtc
    15001 ccctggctga gttcacgctc aatgtacgga tacatggcgc gcactaccgc caccggatcg
    15061 gcatcggtat ccgaggaacc aaagatctcc cggtctaccg ccgccagaat acccggattc
    15121 tgatacgcct cgcgcccgac catcacgcca tccatatgtt gcaggtgtgc tttggcctct
    15181 tccagcgact tgataccacc gttaatcgac attgtcagat gcggaaagtc acgcttcagt
    15241 tgatacacac gcggataatc gagcggcggg atttcacggt tttctttcgg gcttaacccc
    15301 gaaagccagg ctttacgtgc gtggatgata aacatctcac actcgccttt gccggaaacg
    15361 gtgttgatga aatcgcagag aaattcatag ctgtcctggt catcgatgcc aatacgcgtt
    15421 ttcaccgtca ctggaatcga caccacatcg cgcatcgctt tcacgcagtc ggcaaccagc
    15481 tgcgcattac ccatcagaca cgcaccaaac atgccgttct gcacccggtc agacgggcag
    15541 ccgacattca ggttgatctc atcatatcca cgcgcttctg ccagcttcgc acactgtgcc
    15601 agcgccgccg gatcgctacc gccgagttgc aacgctaccg gatgttcttc ttcactgtac
    15661 gccaggtaat cacctttacc gtgaataatc gcccctgtgg tcaccatttc ggtatacagc
    15721 aacgtattgc gggaaagcag acgcaagaaa tagcggcaat gtctgtccgt ccagtcgagc
    15781 attggtgcaa cgctaaaccg accactccag tgaacgtcag ttttttcagg cattgcgctg
    15841 gtttggttga ttttttgcat ttcagaatta ccgtgcattt tcaaatgtag agattatttt
    15901 atcgatatat catggggtta tgttattcag catcactatt caggaggctc aatagcgggg
    15961 tactatacca taacaacagg aagcgcctgt ctcattgcaa aagaaaattg agatcctctc
    16021 aaggcatgaa gctctcacga agtgatggaa ataatcttat tagccgttaa ctttgttaag
    16081 gccaatgata aacaatccag gttcgacgat aaataaaaaa tcacacatta aactctggtg
    16141 atatatctcc ctgctaatag cattgataga gaaaaaagaa cccaataaat attgggtcct
    16201 tttatataat gccttccata ctatcgaaga acttcacata ttatttctcc gatttaaccc
    16261 cgaacaaatc ataaattaat ttagaagtgt ctgtaagtat tttaatctct tcctttgagg
    16321 ttgggtcaaa cgacttcgcg aagctaatta attgtggtgc agcatctcgc attttgctta
    16381 aaatatcagg atcgagcgtt ccttcattca ccagatgtga catcttatcc agatagccat
    16441 caaaattcat ttctccccca tcagccagac gcttcatctc tcccaggtac ttcttcatat
    16501 cacgttgaga taatttttca aactgagctt tcaggtagct ctcattattt tcattaatat
    16561 acactgtttc tgataaatct gcgtaggcac tagaatatga aagtgaaatt aacagcgaag
    16621 caaataattt taagctgttg tcatcatgtt cctggcaggc attaacaaac gttaaaaaac
    16681 cagaacctat tgcttgaaaa tgtatattcg ctaaaggttc attatcctta gattcctcat
    16741 aaaatagctg aaccgtggag ggaaggccat tcttagtttc tacatcaaaa gtacattgtt
    16801 caatgggagt cgcatcttta ttatccccag gattacatat actattaatc gcgaaatgaa
    16861 aaatagcccg gtcaacaact gatgcaataa agtcacgatc gtttaaatcc tcatcagtgc
    16921 aatcgtcaat atacttattg acccacattt tgagatcatt tatttgctca attaaaaaca
    16981 cattttttta cgtgcatgcc ataaattatg cgtgattcgt tgaataaaca aatctatttt
    17041 tgcaataatt atatcccgat gacgcagcgc tgcataatca atagatttac catatatatt
    17101 atttaattta cctttgattg aacctctatt atctaattta tctttgattg catcttcatt
    17161 atcgaattga tttgaattga gtgttttatt aaagtatcca gttgcaccgg gtattatctt
    17221 caacatgatt catctacctt ttttgtcgta ccgtgtcctc cttataattc ttttgccatc
    17281 tggtcactat caaaaatcgc tcataatttc tgaactacag gtatgcgttt aaacccagtt
    17341 aaataaatag cgagttaact tgaagcaatt tatttaaaaa tgaattatta attattatcg
    17401 tgttgataat ggtacgacaa ggcaacataa caccttacct acaaaacaga aaatcaacat
    17461 tacggcaaca ataagggttc tcggtgtttt tcctggaaca tggtaaagta aggacattct
    17521 taacccccac tttgaggtgc ccgatggaaa agaccacaac gcaggagtta ttagcgcagg
    17581 ctgaaaaaat ctgcgcgcag cgtaatgtgc gcctgacccc acagcgcctg gaagtgttgc
    17641 gcctgatgag tctgcaggat ggcgctatca gcgcttatga tctgcttgat ttactgcgcg
    17701 aagctgaacc gcaagccaag ccgccaacgg tttatcgcgc gctggatttt ctgcttgagc
    17761 aaggttttgt gcataaggtg gaatccacca acagttatgt gctctgtcat ctgttcgacc
    17821 agcccacgca tacgtcagcc atgtttatat gcgatcgctg cggcgcggtg aaagaagagt
    17881 gtgcagaagg cgtggaagac attatgcata cgctggcggc aaaaatgggg tttgccctgc
    17941 ggcataatgt gattgaagca catgggctct gtgcggcatg cgtggaagtg gaagcgtgtc
    18001 gtcatcctga acagtgccag catgatcact ctgtgcaggt gaaaaagaaa ccgcgttaag
    18061 agggcgtaca tccttgtaca cgtcgggcag gagggattaa ttaccagcga tattcattgc
    18121 gggtttccca atccacgacc tctttttctg cctgatcttt ctgataacca taacgttcct
    18181 ggattttacc gaccagttga tcacgtttac cttcaatgat cgtcatatca tcatcggtca
    18241 gtttgcccca ttgctctttc actttacctt taaactgttt ccagttaccg ccggcttcat
    18301 ctttattcat aatcaagacc tcatcgttag gttgtgaatg agagtacgtt cacttttctt
    18361 ctgaacgtga gattaagtgt agtcagcaat ttggcttagg ataatttatt cagaattttt
    18421 aaccgtcacg ttgcggcaaa ccaggtaccg ttacgccagt gacgccgcca gatagccgcc
    18481 agagaaagcc cgcgcaacgc cagaaagacg gttaatgcca gccacaaacc atgattaccc
    18541 agccacggca gcgtaaggag cgtcagcgca aaacctgcgg cggccaccgc catactgtta
    18601 cgcatttcgg cggcacgcgt tgcgcctata aacatgccgt ccagcagata acaccagacg
    18661 ccaaccaacg gcaaaatcac ctgccagata agatagcggt cagccagctg ctgaatctgg
    18721 gttaacgacg tcagcaacgc aatgatgtgt tcccctgcca gcaaataaac caccgaaaat
    18781 agtaacgcta cgatccccga ctggcggcac gctgcccgcc agacatccag caactggcta
    18841 ccgtcgcgcg caccgtatgc ctgaccggaa tgtgcttcaa ccgcgtaggc aaaaccatcc
    18901 agcgcatagg cggtaaaggt gagtagcgtc atcagaaccg cgtttacagc gataatgtca
    18961 ctccccagtc gcgcgccaag tacggtgatc gcgccgaaac agagttgcaa caacagcgaa
    19021 cgcagcatga tatcgcggtt aagcgccagc aagcgacgga agtttcctcg ccaggcagtt
    19081 ttcgacattt cgccggaaat tccgcgtagt ttgaggattt tacgcaccat tagcagacca
    19141 atcagcaatg ttgcatattc cgcaataacc gtcgccagag ccgcgccctg cacgttcata
    19201 tgcagcccca tcactagcca gacatccagc acaatgttga ggatattgcc gaccactaac
    19261 aaaattactg gcgcacgggc atattgcacg ccgagtaacc aaccgagtaa taccagattc
    19321 gccagcgacg ccggtgcgct taaccagcgg atttcaagaa agcgccgcgc ctgttccagg
    19381 actgcttcac taccgccaac aatatgcaga gccagatcga taatcggtgt acgcagcagc
    19441 gcaattaacg ccccagcccc caacgccaac agcaacggtt gcaccagcgc acgggctaat
    19501 gcctgaggat ttttggcacc ataagcctgc gcagtcagcc cggtggtgct catgcgtaaa
    19561 aacagcaaca gcataaagag aaagctggtc gccgttgcac caaccgccac gccgcccaaa
    19621 taaaccgggc tatcaagatg accaattacc gccgtatcga ccagtcccag caacggaacg
    19681 gtgatattgg agaaaatcat gggtaaggcg agatgccaga gtgctttatc agatgaagtg
    19741 aggaatgcca tgcagacaag cctgatgaag agagatgaaa aacaaaccgc gacgcctggc
    19801 ggcatcgcgg tctcagagat atgttacagc cagtcgccgt tgcgaataac cccaaccgcc
    19861 agcccttcaa tggtgaagct ctgctgacga aggtcaacga caattggttt aaactcgcta
    19921 ttttctggca acagttcgac tttattaccc tgttttttca ggcgcttaac ggtaacttcg
    19981 tcatcaatac gtgcgacaac gacctgaccg ttacgtacat cctgagtttt atgcactgcc
    20041 agcaagtcac catccataat gccgatatct ttcatcgaca tcccgctgac gcgcagcagg
    20101 aaatcagcat tcggcttgaa caaggaagga tcgacctgat aatgaccttc aatatgctgc
    20161 tgcgccagaa gcggttcacc ggcagccaca cgacctacca gcggcaaccc ttcttcctct
    20221 tcctgcaaca gacgaatccc gcgtgatgcg ccggaaacaa tttcaataac gcctttgcgt
    20281 gccagcgcct tcagatgttc ttcagccgcg tttggggaac ggaaccccaa acgctgcgcg
    20341 atttccgcac gcgtcggcgg catacctgtc tggctgatgt gatcacggat gagatcaaac
    20401 acctcttgtt gcctggccgt taacgctttc attccgcccc ctgggtgtat atacagttat
    20461 gctgtgagta tatacagcaa aaggcgattt tggaaccata aactgcacaa taaaccagag
    20521 atttatcgaa atctggaagg gttatccaaa atgcgaccat aacaggatgc accaggttat
    20581 cacggcgacg ataatcgcaa tcagcaccgc ggcggatccc atatctttag cgcgtccgga
    20641 aagctcatgg tattcagagc caattcggtc aaccactgct tcgatggcgc tattgagaat
    20701 ttccacaatc atcaccagca tcacggagct gataagcagc acgcgggtaa ttgcgtccac
    20761 atccagccag caggcgatga ccaccgccaa caataccgct acgccttcct gacggaatgc
    20821 cgcttcgttg atccatgcag cgcgtaaacc tttccaggaa tagccagcag ctttgataat
    20881 tcgggtgaat ccagtggtat tattggccat tgaaagaacc tttttacatt atgagcgtca
    20941 atatcagtgt accgttaaat ccgatgattc gggtttgcgt aacgccgcgc gaaacatgag
    21001 cggataccac agaatttccc atgactttct gctatccttg ccgcgcattt gcattattaa
    21061 ccagaggctt tacatcgttt atgtccggct ggccacgaat ttactacaaa ttactgaatt
    21121 taccattaag catcctggta aaaagcaagt ctattccggc agatcctgcc ccggaactgg
    21181 ggctggatac ctctcgtcca attatgtacg ttttaccgta caactcgaaa gcagatttgc
    21241 tgacgttacg cgcccagtgt ctggctcatg acttgcctga cccgttagag ccgctagaaa
    21301 tcgacggcac gctactgccg cgctatgtgt tcattcacgg cgggccgcgt gtattcacct
    21361 attacacgcc gaaagaagag tctattaagc tgttccacga ctatctcgat ttgcaccgta
    21421 gcaacccaaa tctggatgtg cagatggtgc cagtatcggt gatgtttggt cgcgcgccgg
    21481 ggcgtgaaaa aggcgaagta aacccgccgc tgcgtatgct taacggcgta cagaaatttt
    21541 tcgccgtact gtggctcggt cgcgacagtt ttgtgcgttt ctcgccgtca gtttcgctgc
    21601 gccgtatggc ggatgaacac ggcacggata aaactatcgc acagaaactg gcgcgcgtgg
    21661 cgcgtatgca ctttgcccgt caacgtctgg ctgccgtagg cccacgtctg cctgctcgtc
    21721 aggatctgtt taataagctg ctcgcctccc gcgccattgc caaagcggta gaagatgaag
    21781 cgcgcagcaa aaaaatctcc catgaaaaag cgcagcagaa cgcgattgcg ctgatggaag
    21841 agattgcggc gaatttctct tacgagatga tccgcctgac tgaccgcatt ctgggcttca
    21901 cctggaaccg actttatcag ggcatcaacg tccataacgc cgagcgcgtt cgccagctgg
    21961 cccacgacgg tcatgagctg gtgtatgtgc cttgccaccg cagtcacatg gactacctgc
    22021 tactttctta cgtgctgtac caccaggggc tggtgccgcc gcatatcgcc gccgggatca
    22081 acctgaactt ctggccggcc gggccgattt tccgccgtct gggggcgttc tttattcgcc
    22141 gtacttttaa aggcaataaa ctttattcca ccgttttccg tgagtatctc ggcgaactgt
    22201 tcagccgtgg ttattccgtc gagtacttcg tggaaggcgg tcgttcccgc acggggcgtt
    22261 tgctggatcc gaaaaccggt acgctgtcga tgaccattca ggcgatgctg cgtggcggca
    22321 cgcgtccgat tacgctgatt ccgatctata tcggttatga gcacgtcatg gaagtgggta
    22381 cttacgccaa agaactgcgc ggcgcgacga aagagaaaga gagcctgccg cagatgctgc
    22441 gcggtttaag caagctgcgt aatctcggtc agggttacgt caacttcggt gaaccaatgc
    22501 cgttgatgac ctaccttaac cagcacgtac cagactggcg tgaatctatc gatcccatcg
    22561 aagcggtgcg tccggcctgg ttaacgccga cggtcaataa tattgctgcc gatctgatgg
    22621 tacgcattaa caacgcaggc gcggcaaacg ccatgaacct gtgttgtact gcgcttctgg
    22681 catcacgtca gcgctcactc acccgcgagc agttaaccga gcaactcaac tgctacctgg
    22741 atctgatgcg caacgtacct tactccacgg actctaccgt tccttcagcc agcgccagcg
    22801 agcttatcga tcacgcgctg caaatgaaca agtttgaagt cgagaaagac actatcggcg
    22861 acatcatcat tctgccgcgc gagcaagcgg tgctgatgac ctactatcgc aacaacattg
    22921 cgcatatgtt ggtgctgcct tcgctgatgg cggcaattgt cacccagcat cgccacatct
    22981 cccgcgacgt attgatggag cacgtcaatg tgctttaccc aatgctgaaa gcggagctgt
    23041 tcctgcgctg ggatcgcgac gagttgccgg acgttattga tgcgctggca aatgagatgc
    23101 aacgtcaggg gctgattacc ctgcaagatg atgagttgca tatcaacccg gcgcattctc
    23161 gccccctaca gctgctggcc gcaggcgcgc gcgaaacgct gcaacgttat gccatcacct
    23221 tctggttgtt gagtgccaac ccgtcgatca accgcggtac gctggagaaa gagagccgca
    23281 ccgttgcgca acgtctctcc gtgctgcacg gcatcaacgc gccggagttc ttcgacaagg
    23341 cggtgttcag ttctctggtg ctgacgctgc gtgatgaagg gtatatcagc gatagcggcg
    23401 acgccgaacc ggcagaaacg atgaaggttt atcagttgct ggcggagttg attacatcag
    23461 acgtgcgttt gacgattgag agtgcgacgc agggcgaagg gtaatcagag agaattgccg
    23521 gatgcggcga aaacgcctta tccggccttc cataatctgc aaattcaata aattgcgatg
    23581 tgttgtgtag gcctgagaag cgcagcgcat caggcaaccc agaagaaagc cggatgatca
    23641 tccggctttt ttacatcatc agaaatgcca gtaactcatt gccagtccta aaaacagtac
    23701 cagaccaaca tagttattat tcataaatgc tttaaagcag gcttcacgct cgcggttggc
    23761 aatcagtttt tgttgataaa caaacagcgc gccagccacc agaattgacc agtaatatcc
    23821 ccagcctaag ccatttaact caccgatgat cgccatcagt gccagtacgc caatctgcaa
    23881 aataccaata atcaatttat cgtattggcc gaacaggatt gcggtggatt taataccaat
    23941 cttcacatca tcgtcacggt caaccattgc atactgcgtg tcgtaagcca ccgcccagag
    24001 aatattggcg aggaacatta accagcaact caatggcacc gactcactca cagcggcaaa
    24061 agccattgga atcgaccagc caaacgccgc gcccagcacc acttgcggga gatgggtata
    24121 ccgcttcata aacgggtaca cccacgccaa cgccagagcg gcaatcgaca acagaatggt
    24181 catcgtattc agcgtcagca ccagtaaaaa tgaaatcaat accagcacta caaacagcgc
    24241 gcgcgcctct ttctccgtta ccgcgccgct gggaagcggt cggttcgccg tgcgcttaac
    24301 atgaccatca aacttgcggt cagcataatc attcaccaca catccggcag cgcgcatcag
    24361 ccagacaccc gcgacaaaca ccgccaggat ccagagctgg ggaacgcccg gtgtcgccac
    24421 ccacaacgcc cataatgttg gccagagcag cagtaacgcg ccaattggct tatccgtacg
    24481 cattaagcga tgaaacgcca gcagcttatt ctgcgtcaga ctccactcca tatttttttc
    24541 ctcttagtac aacggtgacg ccggtaaaaa cagttctgtt agcaacagcg gtttaccgct
    24601 taatcgcagg cgggaacgtc gcccccacag tccggcatca cggcctatct caataaagtc
    24661 ccgggttaat gtcgatgatg tgaacagata gcgtcctagc ggcgttttac ccaatttttg
    24721 taacgccagc tccggcccgc ttaacgttga cacaggaacg acggtacgac cggcaagcca
    24781 cggttcacca tcggcacata acaaaatttc acgtaaccag taacgagact ctttcggcag
    24841 cagcggcagt tcttcgggga tttcattctg ctcgacaaac ccttcgcgga tcatcgtcac
    24901 gctcaccgtt tttccctgct gttcaaaacg ttttgtcatg gaatcttcca gcaacagcca
    24961 gtcgagcagt tgcgaatcca gggcagggat ctctttaaaa tagcgcagcg cacgcagttg
    25021 cgttaacgcg gggtgtgaca tgccgaactc tccgttacat aacgtaaagg cattgtatcg
    25081 cagaaaagtg tttacgggag atgtaaagca aattaagtga tctaatgcgc aacaaaaacg
    25141 caacaatcgc atgaagtggt aaaacgctgt tgaaccttac gctttgccga tgcccgtgct
    25201 ggcgagggca accgtagggg catcaccgcc catgatgcca ggaacggtga tgttttcctt
    25261 tacttaaata cctatattct ttatacgagt agctcgccaa ctcgcagtaa aaattgggaa
    25321 aagcgcatat ttaccatgag atttggagct aaaaagtgtt tcctgagtaa gattaatagg
    25381 caacatctta tgtctggatt tatagataca cgtggaattc caattggtat cttttgtagg
    25441 attcaacata tc
//
LOCUS       DAESHI010000053        25284 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.053, whole genome shotgun sequence.
ACCESSION   DAESHI010000053 DAESHI010000000
VERSION     DAESHI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25284
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.053"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            17..268
                     /locus_tag="KW011_002268"
                     /pseudo
     CDS             17..268
                     /locus_tag="KW011_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="involved in type II protein secretion; incomplete;
                     partial in the middle of a contig; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
     gene            506..817
                     /gene="rpsJ"
                     /locus_tag="KW011_002269"
     CDS             506..817
                     /gene="rpsJ"
                     /locus_tag="KW011_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBH7660584.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            850..1479
                     /gene="rplC"
                     /locus_tag="KW011_002270"
     CDS             850..1479
                     /gene="rplC"
                     /locus_tag="KW011_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBH7660585.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            1490..2095
                     /gene="rplD"
                     /locus_tag="KW011_002271"
     CDS             1490..2095
                     /gene="rplD"
                     /locus_tag="KW011_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBH7660586.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2092..2394
                     /gene="rplW"
                     /locus_tag="KW011_002272"
     CDS             2092..2394
                     /gene="rplW"
                     /locus_tag="KW011_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBH7660587.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            2412..3233
                     /gene="rplB"
                     /locus_tag="KW011_002273"
     CDS             2412..3233
                     /gene="rplB"
                     /locus_tag="KW011_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBH7660588.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3250..3528
                     /gene="rpsS"
                     /locus_tag="KW011_002274"
     CDS             3250..3528
                     /gene="rpsS"
                     /locus_tag="KW011_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBH7660589.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            3543..3875
                     /gene="rplV"
                     /locus_tag="KW011_002275"
     CDS             3543..3875
                     /gene="rplV"
                     /locus_tag="KW011_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBH7660590.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            3893..4594
                     /gene="rpsC"
                     /locus_tag="KW011_002276"
     CDS             3893..4594
                     /gene="rpsC"
                     /locus_tag="KW011_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBH7660591.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            4607..5017
                     /gene="rplP"
                     /locus_tag="KW011_002277"
     CDS             4607..5017
                     /gene="rplP"
                     /locus_tag="KW011_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBH7660592.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5017..5208
                     /gene="rpmC"
                     /locus_tag="KW011_002278"
     CDS             5017..5208
                     /gene="rpmC"
                     /locus_tag="KW011_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBH7660593.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5208..5462
                     /gene="rpsQ"
                     /locus_tag="KW011_002279"
     CDS             5208..5462
                     /gene="rpsQ"
                     /locus_tag="KW011_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBH7660594.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            5627..5998
                     /gene="rplN"
                     /locus_tag="KW011_002280"
     CDS             5627..5998
                     /gene="rplN"
                     /locus_tag="KW011_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBH7660595.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6009..6323
                     /gene="rplX"
                     /locus_tag="KW011_002281"
     CDS             6009..6323
                     /gene="rplX"
                     /locus_tag="KW011_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBH7660596.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6338..6877
                     /gene="rplE"
                     /locus_tag="KW011_002282"
     CDS             6338..6877
                     /gene="rplE"
                     /locus_tag="KW011_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBH7660597.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            6892..7197
                     /gene="rpsN"
                     /locus_tag="KW011_002283"
     CDS             6892..7197
                     /gene="rpsN"
                     /locus_tag="KW011_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBH7660598.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7231..7623
                     /gene="rpsH"
                     /locus_tag="KW011_002284"
     CDS             7231..7623
                     /gene="rpsH"
                     /locus_tag="KW011_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBH7660599.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            7636..8169
                     /gene="rplF"
                     /locus_tag="KW011_002285"
     CDS             7636..8169
                     /gene="rplF"
                     /locus_tag="KW011_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBH7660600.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8179..8532
                     /gene="rplR"
                     /locus_tag="KW011_002286"
     CDS             8179..8532
                     /gene="rplR"
                     /locus_tag="KW011_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBH7660601.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            8547..9050
                     /gene="rpsE"
                     /locus_tag="KW011_002287"
     CDS             8547..9050
                     /gene="rpsE"
                     /locus_tag="KW011_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBH7660602.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9054..9233
                     /gene="rpmD"
                     /locus_tag="KW011_002288"
     CDS             9054..9233
                     /gene="rpmD"
                     /locus_tag="KW011_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBH7660603.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9237..9671
                     /gene="rplO"
                     /locus_tag="KW011_002289"
     CDS             9237..9671
                     /gene="rplO"
                     /locus_tag="KW011_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBH7660604.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            9679..11010
                     /gene="secY"
                     /locus_tag="KW011_002290"
     CDS             9679..11010
                     /gene="secY"
                     /locus_tag="KW011_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBH7660605.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DATVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11042..11158
                     /gene="rpmJ"
                     /locus_tag="KW011_002291"
     CDS             11042..11158
                     /gene="rpmJ"
                     /locus_tag="KW011_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBH7660606.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11305..11661
                     /gene="rpsM"
                     /locus_tag="KW011_002292"
     CDS             11305..11661
                     /gene="rpsM"
                     /locus_tag="KW011_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBH7660607.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            11678..12067
                     /gene="rpsK"
                     /locus_tag="KW011_002293"
     CDS             11678..12067
                     /gene="rpsK"
                     /locus_tag="KW011_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBH7660608.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12101..12721
                     /gene="rpsD"
                     /locus_tag="KW011_002294"
     CDS             12101..12721
                     /gene="rpsD"
                     /locus_tag="KW011_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBH7660609.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            12747..13736
                     /gene="rpoA"
                     /locus_tag="KW011_002295"
     CDS             12747..13736
                     /gene="rpoA"
                     /locus_tag="KW011_002295"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBH7660610.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            13777..14160
                     /gene="rplQ"
                     /locus_tag="KW011_002296"
     CDS             13777..14160
                     /gene="rplQ"
                     /locus_tag="KW011_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBH7660611.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14267..14635
                     /locus_tag="KW011_002297"
     CDS             14267..14635
                     /locus_tag="KW011_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HBH7660612.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            14646..15071
                     /gene="zntR"
                     /locus_tag="KW011_002298"
     CDS             14646..15071
                     /gene="zntR"
                     /locus_tag="KW011_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HBH7660613.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15127..15345
                     /gene="arfA"
                     /locus_tag="KW011_002299"
     CDS             15127..15345
                     /gene="arfA"
                     /locus_tag="KW011_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HBH7660614.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15342..15752)
                     /gene="mscL"
                     /locus_tag="KW011_002300"
     CDS             complement(15342..15752)
                     /gene="mscL"
                     /locus_tag="KW011_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBH7660615.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(15882..17258)
                     /gene="trkA"
                     /locus_tag="KW011_002301"
     CDS             complement(15882..17258)
                     /gene="trkA"
                     /locus_tag="KW011_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HBH7660616.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17280..18569)
                     /gene="rsmB"
                     /locus_tag="KW011_002302"
     CDS             complement(17280..18569)
                     /gene="rsmB"
                     /locus_tag="KW011_002302"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBH7660617.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQHQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(18615..19562)
                     /gene="fmt"
                     /locus_tag="KW011_002303"
     CDS             complement(18615..19562)
                     /gene="fmt"
                     /locus_tag="KW011_002303"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBH7660618.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(19577..20086)
                     /gene="def"
                     /locus_tag="KW011_002304"
     CDS             complement(19577..20086)
                     /gene="def"
                     /locus_tag="KW011_002304"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBH7660619.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20216..21340
                     /gene="dprA"
                     /locus_tag="KW011_002305"
     CDS             20216..21340
                     /gene="dprA"
                     /locus_tag="KW011_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBH7660620.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21312..21785
                     /locus_tag="KW011_002306"
     CDS             21312..21785
                     /locus_tag="KW011_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HBH7660621.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            21814..22356
                     /locus_tag="KW011_002307"
     CDS             21814..22356
                     /locus_tag="KW011_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HBH7660622.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22361..22933
                     /gene="tsaC"
                     /locus_tag="KW011_002308"
     CDS             22361..22933
                     /gene="tsaC"
                     /locus_tag="KW011_002308"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HBH7660623.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            22938..23756
                     /gene="aroE"
                     /locus_tag="KW011_002309"
     CDS             22938..23756
                     /gene="aroE"
                     /locus_tag="KW011_002309"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBH7660624.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            23753..24010
                     /locus_tag="KW011_002310"
     CDS             23753..24010
                     /locus_tag="KW011_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HBH7660625.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(23986..24540)
                     /locus_tag="KW011_002311"
     CDS             complement(23986..24540)
                     /locus_tag="KW011_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBH7660626.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            25013..>25284
                     /locus_tag="KW011_002312"
     rRNA            25013..>25284
                     /locus_tag="KW011_002312"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         6442 a         5993 c         6617 g         6232 t
ORIGIN      
        1 gcttctttaa tgggcgcttt ggatagtaaa agcagaagtc actgtagagg ctgaaacgcc
       61 cgcttttaaa caatcaacac aacactattt cttcaaaaaa cagccactcc ccgtcgtcga
      121 atccgtcgag gaggaagatg acccagacgt cgcggttgag aatgctccct cttcttctga
      181 agacgaagaa aatactgtaa aagaaagcga cgagaaagcc ggtttaagag agcgtgtcaa
      241 aaatgcgctg aacgaacttg agagataacc cgaaggctgt ttactttact agcaatacgc
      301 ttgcgttcgg tggttaagta tgtataatgc gcgggcttgt cgtagttgac agcaggttca
      361 atctgaacct cagttagccg aatttggcta cctaacaatg ctcccaatcg gggagctacg
      421 taagaacggt tacactctcc catcaatcgt aatgggtctg aggagtaatc attttcgttt
      481 ataaaataat tggagctctg gtctcatgca gaaccaaaga atccgtatcc gcctgaaagc
      541 gtttgatcat cgtctgatcg atcaagcaac cgcggaaatc gtcgagactg ccaagcgcac
      601 tggtgcgcag gtccgtggtc cgatcccgct gccgacacgc aaagagcgct tcactgttct
      661 gatctccccg cacgtcaaca aagacgcgcg cgatcagtac gaaatccgta ctcacttgcg
      721 tctggttgac atcgttgagc caaccgagaa aaccgttgat gctctgatgc gtctggatct
      781 ggctgccggt gtagacgtgc agatcagcct gggttaatca ggtcattgag cgattgagag
      841 gttgaaacaa tgattggttt agtcggtaaa aaagtgggta tgacccgtat cttcacagaa
      901 gacggcgttt ctatcccagt aaccgtaatc gaagttgaag caaaccgcgt tactcaggtt
      961 aaagacctgg ctaacgatgg ctaccgtgct attcaggtga ccaccggtgc taaaaaagct
     1021 aaccgtgtga ccaagcctga agctggccac ttcgctaaag ctggcgtaga agctggccgt
     1081 ggtctgtggg aattccgcct ggctgaaggc gaagagttca ctgtaggtca gagcattagc
     1141 gttgaactgt ttgctgaggt taaaaaagtt gacgtaactg gcacctctaa aggtaaaggt
     1201 ttcgcaggta ccgttaagcg ctggaacttc cgtacccagg acgctactca cggtaactcc
     1261 ttgtctcacc gcgttccggg ttctatcggt cagaaccaga ctccgggcaa agtgttcaaa
     1321 ggcaagaaaa tggcaggtca gatgggtaac gaacgtgtaa ccgttcagag ccttgacgta
     1381 gtacgcgttg acgctgagcg caacctgctg ctggttaaag gtgctgtccc gggtgcaacc
     1441 ggtagcgacc tgatcgttaa accagctgtg aaggcgtaag gagatagcaa tggaattagt
     1501 attgaaagac gcgcagagcg cgctgactgt ttccgaaact accttcggtc gtgatttcaa
     1561 cgaagcgctg gttcaccagg ttgttgttgc ttatgcagct ggtgctcgtc agggtactcg
     1621 tgctcagaag actcgtgctg aagtaactgg ttccggtaaa aaaccgtggc gccagaaagg
     1681 caccggccgt gcgcgttctg gttctatcaa gagcccgatc tggcgttctg gtggcgtgac
     1741 ctttgctgct cgtccgcagg accacagtca aaaagttaac aagaagatgt accgcggcgc
     1801 gctgaaaagc atcctgtccg aactggtacg tcaggatcgt ctgatcgttg tcgagaagtt
     1861 ctctgtagaa gcgccgaaaa ctaagctgct ggcacagaaa ctgaaagaca tggctctgga
     1921 agatgtgctg atcatcaccg gtgagctgga cgaaaacctg ttcctggctg cgcgcaacct
     1981 gcacaaggtt gacgtacgcg atgcaactgg tatcgacccg gttagcctga tcgccttcga
     2041 caaagtcgta atgactgctg atgctgttaa gcaagttgag gagatgctgg catgattcgt
     2101 gaagaacgtc tgctgaaggt gctgcgtgca ccgcacgttt ctgaaaaagc gtctactgcg
     2161 atggaaaaat ccaacaccat cgtactcaaa gttgctaaag acgcgaccaa agcagaaatc
     2221 aaagctgctg tgcagaaact gtttgaagtc gaagtcgaag tcgttaacac cctggtagtt
     2281 aaagggaaag ttaaacgtca cggacagcgt atcggtcgtc gtagcgactg gaaaaaagct
     2341 tacgtcaccc tgaaagaagg ccagaatctg gacttcgttg gcggcgctga gtaagtcgga
     2401 ggagtaatac aatggcagtt gttaaatgta aaccgacatc tccgggtcgt cgccacgtag
     2461 ttaaagtggt taaccctgag ctgcacaagg gcaaaccttt tgctccgttg ctggaaaaaa
     2521 acagcaaatc cggtggtcgt aacaacaatg gccgtatcac cactcgtcat atcggtggtg
     2581 gccacaagca ggcttaccgt attgttgact tcaaacgcaa caaagacggt atcccggcag
     2641 ttgttgaacg tcttgagtac gatccgaacc gttccgcgaa catcgcgctg gttctgtaca
     2701 aagacggtga acgccgttac atcctggccc ctaaaggcct gaaagctggc gaccagattc
     2761 agtctggcgt tgatgctgca atcaaaccag gtaacaccct gccgatgcgc aacatcccgg
     2821 ttggttctac tgttcataac gtagaaatga aaccaggtaa aggcggtcag ctggcacgtt
     2881 ccgctggtac ttacgttcag atcgttgctc gtgatggtgc ttatgtcacc ctgcgtctgc
     2941 gttctggtga aatgcgtaaa gtagaagcag actgccgtgc aactctgggc gaagttggca
     3001 atgctgagca tatgctgcgc gttctgggta aagcaggtgc tgcacgctgg cgtggtgttc
     3061 gtccgaccgt tcgcggtacc gcgatgaacc cggtagacca cccacatggt ggtggtgaag
     3121 gtcgtaactt tggtaagcac ccggtaactc cgtggggcgt tcagaccaaa ggtaagaaga
     3181 cccgcagcaa caagcgtact gataaattca tcgtacgtcg ccgtagcaaa taattttaga
     3241 ggataagcca tgccacgttc tctcaagaaa ggtcctttta ttgacctgca cttgctgaag
     3301 aaggtagaga aagcggtgga aagcggagac aagaagcccc tgcgcacttg gtcccgtcgt
     3361 tcaacgatct ttcctaacat gatcggtttg accatcgctg tccataatgg tcgtcagcac
     3421 gttccggtat ttgtgaccga cgaaatggtc ggtcacaaac tgggtgaatt cgcaccgact
     3481 cgtacttatc gcggccacgc tgctgataaa aaagcgaaga agaaataagg taggaggaag
     3541 agatggaaac tatcgctaaa catcgccatg ctcgttcttc tgctcagaag gttcgccttg
     3601 ttgctgacct gattcgcggt aagaaagtgt cgcaggctct ggatattttg acctacacca
     3661 acaagaaagc ggctgtactg gtcaagaaag ttctggaatc tgccattgct aacgctgaac
     3721 acaacgatgg cgctgacatt gacgatctga aagttacgaa aattttcgta gacgaaggcc
     3781 cgagcatgaa gcgcattatg ccgcgtgcaa aaggtcgtgc agatcgcatc ctgaagcgca
     3841 ccagccacat cactgtggtt gtgtccgatc gctgagactc tggagactag caatgggtca
     3901 gaaagtacat cctaatggta ttcgcttggg tattgtaaag ccatggaact ctacctggtt
     3961 tgcgaacacc aaagaattcg ctgacaacct ggacagcgat tttaaagtac gtcagtacct
     4021 gactaaggaa ctggctaaag cgtccgtatc tcgtatcgtt atcgagcgtc cggctaagag
     4081 catccgtgta accattcaca ctgctcgccc gggtatcgtt atcggtaaaa aaggtgaaga
     4141 cgtagaaaaa ctgcgtaagg tcgtagcgga catcgctggc gttcctgcac agatcaacat
     4201 cgccgaagtt cgtaagcctg aactggacgc aaaactggtt gctgacagca tcacttctca
     4261 gctggaacgt cgcgttatgt tccgtcgtgc tatgaagcgt gctgtacaga acgcaatgcg
     4321 tctgggcgct aaaggtatta aagttgaagt tagcggccgt ctgggcggcg cggaaatcgc
     4381 acgtaccgaa tggtaccgcg aaggtcgcgt accgctgcac actctgcgtg ctgacatcga
     4441 ctacaacacc tctgaagcgc acaccactta cggtgtaatc ggcgttaaag tgtggatctt
     4501 caaaggcgag atcctgggtg gtatggctgc tgttgaacaa ccggaaaaac cggctgctca
     4561 gcctaaaaag cagcagcgta aaggccgtaa ataaggagcg tcgctgatgt tacaaccaaa
     4621 gcgtacaaaa ttccgtaaaa tgcacaaagg ccgtaaccgc ggtctggcgc agggtacgga
     4681 tgttagcttc ggcagcttcg gtctgaaagc tgttggccgt ggtcgtctga ctgcccgtca
     4741 gatcgaagca gcacgtcgtg ctatgacccg tgcagttaag cgtcaaggta agatctggat
     4801 ccgtgtgttc ccggacaaac cgatcactga aaagccgctg gcagtgcgta tgggtaaagg
     4861 taaaggtaac gtggagtatt gggttgcctt gattcagccg ggtaaagtcc tgtatgaaat
     4921 ggacggtgtt ccggaagagc tggcccgtga agcattcaag ctggcagcag cgaaactgcc
     4981 gattaaaacc acctttgtaa ctaagacggt gatgtaatga aagcaaaaga gctgcgtgag
     5041 aagagcgttg aagagctgaa caccgagctg ctgaacctgc tgcgtgagca gttcaacctg
     5101 cgtatgcagg ctgcaagtgg ccagctgcaa cagtctcacc tgttgaagca agtgcgtcgc
     5161 gatgtcgcac gcgttaagac tttactgaac gagaaggcgg gtgcgtaatg accgataaaa
     5221 tccgtactct gcaaggtcgc gttgttagcg acaaaatgga gaaatccatt gttgttgcta
     5281 tcgaacgttt tgtgaaacac ccgatctacg gtaaattcat caagcgtacg accaaactgc
     5341 acgtacatga cgagaacaac gaatgcggta tcggtgacgt ggttgaaatc cgcgaatgcc
     5401 gtccgctgtc caagactaaa tcctggacgc tggttcgcgt tgtagagaaa gcggttctgt
     5461 aatacagtac actctctcga tacgaataaa cggctcagaa atgagccgtt tattttttct
     5521 acccatatcc ttgaagcggt gttataatgc cgcgccctcg atatggggat ttttaacgac
     5581 ctgattttcg ggtctcagta gtagttgaca ttagcggagc actaaaatga tccaagaaca
     5641 gactatgctg aacgtcgccg acaactccgg tgcacgtcgc gtaatgtgta tcaaggttct
     5701 gggtggctcg caccgtcgct acgcaggcgt aggcgacatc atcaagatca ccatcaaaga
     5761 agcaattccg cgtggtaagg tcaaaaaagg tgatgtgctg aaggcggtag tggtgcgcac
     5821 caagaagggt gttcgtcgcc cggacggttc tgtcattcgc ttcgatggta atgcttgtgt
     5881 tcttctgaac aacaacagcg agcagcctat cggtacgcgt atttttgggc cggtaactcg
     5941 tgagcttcgt agtgagaagt tcatgaaaat tatctctctg gcaccagaag tactctaagg
     6001 agcgaatcat ggcagcgaaa atccgtcgtg atgacgaagt tatcgtgtta accggtaaag
     6061 ataaaggtaa acgcggtaaa gttaagaatg tcctgtcttc cggcaaggtc attgttgaag
     6121 gtatcaacct ggttaagaaa catcagaagc cggttccggc cctgaaccaa ccgggtggca
     6181 tcgttgaaaa agaagccgct attcaggttt ccaacgtagc aatcttcaat gcggcaaccg
     6241 gcaaggctga ccgtgtaggc tttagattcg aagacggcaa aaaagtccgt ttcttcaagt
     6301 ctaacagcga aactatcaag taatttggag tagtacgatg gcgaaactgc atgattacta
     6361 caaagacgaa gtagttaaaa aactcatgac tgagtttaac tacaattctg tcatgcaagt
     6421 ccctcgggtc gagaagatca ccctgaacat gggtgttggt gaagcgatcg ctgacaaaaa
     6481 actgctggat aacgcagcag cagacctggc agcaatctcc ggtcaaaaac cgctgatcac
     6541 caaagcacgc aaatctgttg caggcttcaa aatccgtcag ggctatccga tcggctgtaa
     6601 agtaactctg cgtggcgaac gcatgtggga gttctttgag cgcctgatca ctattgctgt
     6661 acctcgtatt cgtgacttcc gtggcctgtc cgctaagtct ttcgacggtc gtggtaacta
     6721 cagcatgggt gtccgtgagc agatcatctt cccagaaatc gactacgata aagtcgaccg
     6781 cgttcgtggt ttggatatta ccattaccac tactgcgaaa tctgacgaag aaggccgcgc
     6841 tctgctggct gcctttgact tcccgttccg caagtaaggt agggttacta aatggctaag
     6901 caatcaatga aagcacgcga agtaaaacgc gtagctttag ctgataaata cttcgcgaaa
     6961 cgcgctgaac tgaaagcgat catctctgat gtgaacgctt ccgacgaaga tcgttggaac
     7021 gctgttctca agctgcagac tctgccgcgt gattccagcc cgtctcgtca gcgtaaccgc
     7081 tgccgtcaaa caggtcgccc gcatggtttc ctgcggaagt tcgggttgag ccgtattaag
     7141 gtccgtgaag ccgctatgcg cggtgaaatc ccgggtctga aaaaggctag ctggtaattg
     7201 tcaccaattg aatcacggga gtaaagacag atgagcatgc aagatccgat cgcggatatg
     7261 ctgacccgta tccgtaacgg tcaggccgcg aacaaagctg cggtcaccat gccttcctcc
     7321 aagctgaaag tggcaatcgc caacgtgctg aaggaagaag gttttattga agattttaaa
     7381 gttgaaggcg acaccaagcc tgaactggaa cttactctga agtatttcca gggcaaagct
     7441 gttgtagaaa gcattcagcg tgtcagccgc ccaggtctgc gcatctataa acgtaaagat
     7501 gagctgccga aagttatggc gggtctgggt atcgcagttg tttctacctc taaaggtgtt
     7561 atgactgatc gtgcagcgcg ccaggctggt cttggtggcg aaattatctg ctacgtagcc
     7621 taatcggagg aaaaaatgtc tcgtgttgct aaagcaccgg tcgttgttcc tgccggcgtt
     7681 gacgtaaaaa tcaacggtca ggttattacg atcaaaggta aaaacggcga gctgactcgt
     7741 actctcaacg atgctgttga agttaaacat gcagataata ccctgacctt cggtccgcgt
     7801 gatggttacg cagacggttg ggcacaggct ggtaccgcgc gtgccctgct gaactcaatg
     7861 gttatcggtg ttaccgaagg cttcactaag aagctgcagc tggttggtgt aggttaccgt
     7921 gcagcggtta aaggcaatgt gattaacctg tctctgggtt tctctcatcc tgttgaccat
     7981 cagctgcctg cgggtatcac tgctgaatgt ccgactcaga ctgaaatcgt gctgaaaggc
     8041 gttgataagc aggtgatcgg ccaggttgca gcggatctgc gcgcctaccg tcgtcctgag
     8101 ccttataaag gcaagggtgt tcgttacgcc gacgaagtcg tgcgtaccaa agaggctaag
     8161 aagaagtaag gtaacactat ggataagaaa tttgctcgta tccgtcgtgc gacccgcgca
     8221 cgccgcaagc tccaggagct gggcgcaact cgcctggtgg tacatcgtac cccgcgtcac
     8281 atttacgcac aggtaattgc accgaacggt tctgaagttc tggtagctgc ttctactgta
     8341 gaaaaagcta tcgctgaaca actgaagtac accggtaaca aagacgcggc tgcagctgtg
     8401 ggtaaagctg tcgctgaacg cgctctggaa aaaggcatca aagatgtatc ctttgaccgt
     8461 tccgggttcc aatatcatgg tcgtgtccag gcactggcag atgctgcccg tgaagctggc
     8521 cttcagttct aaggtagagg tgtaagatgg ctcacatcga aaaacaagct ggcgaactgc
     8581 aggaaaagct gatcgcggta aaccgcgtat ctaaaaccgt taaaggtggt cgtattttct
     8641 ccttcacagc tctgactgta gttggcgatg gtaacggtcg cgttggtttt ggttacggta
     8701 aagcgcgtga agttccagca gcgatccaga aagcgatgga aaaagcccgt cgcaatatga
     8761 ttaacgtcgc gctgaataac ggcactctgc aacaccctgt taaaggtgtt cacacgggtt
     8821 ctcgcgtatt catgcagccg gcttccgaag gtaccggtat catcgccggt ggtgcaatgc
     8881 gcgccgttct ggaagtcgct ggggttcata acgttctggc taaagcatat ggttccacca
     8941 acccgatcaa cgtggttcgt gcaactattg atggcctgga aaatatgaat tctccagaaa
     9001 tggtcgctgc caagcgtggt aaatccgttg aagaaattct ggggaaataa accatggcaa
     9061 agactattaa aattactcaa acccgcagtg caatcggtcg tctgccgaaa cacaaggcaa
     9121 cgctgcttgg cctgggtctg cgtcgtattg gtcacaccgt agagcgcgag gatactcctg
     9181 ctattcgcgg tatgatcaac gcggtttcct tcatggttaa agttgaggag taagagatgc
     9241 gtttaaatac tctgtctccg gccgaaggct ccaaaaaggc gggtaaacgc ctgggtcgtg
     9301 gtatcggttc tggcctcggt aaaaccggtg gtcgtggtca caaaggtcag aagtctcgtt
     9361 ctggcggtgg cgtacgtcgc ggtttcgagg gtggtcagat gcctctgtac cgtcgtctgc
     9421 cgaaattcgg cttcacttct cgtaaagcag cgattacagc cgaagttcgt ctgtctgacc
     9481 tggctaaagt agaaggcggt gttgtagacc tgaacacgct gaaagcggct aacattatcg
     9541 gtatccagat cgagttcgcg aaagtgatcc tggctggcga agtaacgact ccggtaactg
     9601 ttcgtggcct gcgtgttact aaaggcgctc gtgctgctat cgaagctgct ggcggtaaaa
     9661 tcgaggaata agtagcagat ggctaaacaa ccgggattag attttcaaag tgccaaaggt
     9721 ggcttaggcg agctgaaacg cagactgctg tttgttatcg gtgcgctgat tgtgttccgt
     9781 attggctctt ttattccgat ccctggtatt gatgccactg tacttgccaa actgcttgag
     9841 caacagcgag gcaccatcat tgagatgttt aacatgttct ctggtggtgc tctcagccgt
     9901 gcttctatct ttgctctggg gatcatgccg tatatttcgg cgtcgatcat tatccagctg
     9961 ctgacggtgg ttcacccaac gttggcggaa attaagaaag aaggggagtc tggtcgtcgt
    10021 aagatcagcc agtacacccg ctacggtact ctggtgctgg caatattcca gtcgatcggt
    10081 attgctactg gtctgccgaa tatgcctggt atgcaaggcc tggtgattaa cccgggcttt
    10141 gcattctact tcaccgctgt tgtaagtctg gtcacaggaa cgatgttcct gatgtggttg
    10201 ggcgaacaga ttactgaacg aggtatcggc aacggtattt caatcattat cttcgtcggt
    10261 attgtcgcgg gactcccgcc agccattgcc catactatcg agcaagcgcg tcaaggcgac
    10321 ctgcacttcc tcgtgttgct gttggttgca gtattagtat ttgcagtgac gttctttgtt
    10381 gtatttgttg agcgtggtca acgccgcatt gtggtaaact acgcgaaacg tcagcaaggt
    10441 cgtcgtgttt atgctgcaca gagcacacat ttaccgctga aagtgaatat ggcgggggta
    10501 atcccggcaa tcttcgcttc cagtattatt ctgttcccgg cgaccatcgc gtcatggttc
    10561 gggggcggta ctggttggaa ctggctgaca acaatttcgc tgtatttgca gcctgggcaa
    10621 ccgctttatg tgttactcta tgcgtctgca atcatcttct tctgtttctt ctacacggcg
    10681 ttggttttca acccgcgtga aacagcagat aacctgaaga agtccggtgc atttgtacca
    10741 ggaattcgtc cgggagagca aacggcgaag tatatcgata aagtaatgac ccgcctgacc
    10801 ctggttggtg cgctgtatat tacctttatc tgcctgatcc cggagttcat gcgtgatgca
    10861 atgaaagtac cgttctactt cggtgggacc tcactgctta tcgttgttgt cgtgattatg
    10921 gactttatgg ctcaagtgca aactctgatg atgtccagtc agtatgagtc tgcattgaag
    10981 aaggcgaacc tgaaaggcta cggccgataa ttggtcgccc gagaagttac ggagagtaaa
    11041 aatgaaagtt cgtgcttccg tcaagaaatt atgccgtaac tgcaaaatcg ttaagcgtga
    11101 tggtgtcatc cgtgtgattt gcagtgccga gccgaagcat aaacagcgcc aaggctgatt
    11161 ttttcgcata tttttcttgc aaagttgggt tgagctggct agattagcca gccaatcttt
    11221 tgtatgtctg tgcgtttcca tttgagtatc ctgaaaacgg gcttttcagc atggaacgta
    11281 catattaaat agtaggagtg catagtggcc cgtatagcag gcattaacat tcctgatcat
    11341 aagcatgccg taatcgcatt aacttcgatt tatggcgtcg gcaagacccg ttctaaagcc
    11401 atcctggctg cagcgggtat cgctgaagat gttaagatca gtgagctgtc tgaaggacaa
    11461 atcgacacgc tgcgtgacga agttgccaaa tttgtcgttg aaggtgatct gcgccgtgaa
    11521 atcagcatga gcatcaagcg cctgatggat cttggttgct atcgcggttt gcgtcatcgt
    11581 cgtggtctcc cggttcgcgg tcagcgtacc aagaccaacg cacgtacccg taagggtccg
    11641 cgcaaaccga tcaagaaata atcggggtga ttgaataatg gcaaaggcac caattcgtgc
    11701 acgtaaacgt gtaagaaaac aagtctctga cggcgtggct catatccatg cttctttcaa
    11761 caacaccatc gtgactatca ctgatcgtca gggtaacgcg ttgggttggg caacagccgg
    11821 tggttccggt ttccgtggtt ctcgcaaatc cactccgttt gcagctcagg ttgcagcaga
    11881 gcgttgcgct gacgccgtga aagaatacgg catcaagaat ctggaagtta tggttaaagg
    11941 tccgggtcca ggccgcgaat ctactattcg tgctctgaac gccgcaggtt tccgcatcac
    12001 taacattact gatgtgactc cgatccctca taacggttgt cgtccgccga aaaaacgtcg
    12061 cgtataacgc ctcgttttcc aggtttgttg gagaaagaaa atggcaagat atttgggtcc
    12121 taagctcaag ctgagccgtc gtgagggcac cgacttattc cttaagtctg gcgttcgcgc
    12181 gatcgatacc aagtgtaaaa ttgaacaagc tcctggccag cacggtgcgc gtaaaccgcg
    12241 tctgtctgac tatggtgtgc agttgcgtga aaagcaaaaa gttcgccgta tctatggtgt
    12301 gctggagcgt cagttccgta actactacaa agaagcagca cgtctgaaag gcaacaccgg
    12361 tgaaaacctg ttggctctgc tggaaggtcg tctggacaac gttgtatacc gtatgggctt
    12421 cggtgccact cgtgcagaag cacgtcagct ggttagccat aaagcaatta tggtaaacgg
    12481 tcgtgttgtt aacatcgctt cttatcaggt tagtccgaat gacgttgtaa gcattcgtga
    12541 gaaagcgaag aagcagtctc gcgtgaaagc cgctctggag ctggctgagc agcgtgaaaa
    12601 gccaacctgg ctggaagttg atgctggcaa gatggaaggt acgtttaagc gtaagccgga
    12661 gcgttctgat ctgtctgcgg acattaacga acacctgatc gtcgagcttt actccaagta
    12721 aagcttagta ccaaagagag gacacaatgc agggttctgt gacagagttt ctaaaaccgc
    12781 gcctggttga tatcgagcaa gtgagttcga cgcacgccaa ggtgaccctt gagcctttag
    12841 agcgtggctt tggccatact ctgggtaacg cactgcgccg tattctgctc tcatcgatgc
    12901 cgggttgcgc ggtgaccgag gttgagattg atggtgtact acatgagtac agcaccaaag
    12961 aaggcgttca ggaagatatc ctggaaatcc tgctcaacct gaaagggctg gcggtgagag
    13021 ttcagggcaa agatgaagtt attcttacct tgaataaatc tggcattggc cctgtgactg
    13081 cagccgatat cacccacgac ggtgatgtcg aaatcgtcaa gccgcagcac gtgatctgcc
    13141 acctgaccga tgagaacgcg tctattagca tgcgtatcaa agttcagcgc ggtcgtggtt
    13201 atgtgccggc ttctacccga attcattcgg aagaagatga gcgcccaatc ggccgtctgc
    13261 tggtcgacgc atgctacagc cctgtggagc gtattgccta caatgttgaa gcagcgcgtg
    13321 tagaacagcg taccgacctg gacaagctgg tcatcgaaat ggaaaccaac ggcacaatcg
    13381 atcctgaaga ggcgattcgt cgtgcggcaa ccattctggc tgaacaactg gaagctttcg
    13441 ttgacttacg tgatgtacgt cagcctgaag tgaaagaaga gaaaccagag ttcgatccga
    13501 tcctgctgcg ccctgttgac gatctggaat tgactgtccg ctctgctaac tgccttaaag
    13561 cagaagctat ccactatatc ggtgatctgg tacagcgtac cgaggttgag ctccttaaaa
    13621 cgcctaacct tggtaaaaaa tctcttactg agattaaaga cgtgctggct tcccgtggac
    13681 tgtctctggg catgcgcctg gaaaactggc caccggcaag catcgctgac gagtaaccgg
    13741 atcacaggtt aaggttttac tgagaaggat aaggtcatgc gccatcgtaa gagtggtcgt
    13801 caactgaacc gcaacagcag ccatcgccag gctatgttcc gcaatatggc aggttcactg
    13861 gttcgtcatg aaatcatcaa gacgactctg cctaaagcga aagagctgcg ccgcgtagtt
    13921 gagccgctga ttactcttgc caagactgat agcgttgcta atcgtcgtct ggcattcgcc
    13981 cgtactcgtg ataacgagat cgtggcaaaa ctgtttaacg aactgggccc gcgtttcgcg
    14041 agccgtgccg gtggttacac tcgtattctg aagtgtggct tccgtgcagg cgacaacgcg
    14101 ccgatggctt acatcgagct ggttgatcgt tcagagaaag cagaagctgc tgcagagtaa
    14161 tctgaagcaa tgtaaaaaaa cccgccccgg cgggtttttt tatacccgta gtatccccac
    14221 ttatctacaa tagctgtact ctttttgttc atcccctgga gtatttatgt ggttacttga
    14281 ccagtgggca gagcgccata tagcagaagc gcaagcgaaa ggtgagtttg ataacctggc
    14341 aggtagcggc gaaccattga tactggatga tgattctcac gtgccaccgg aattacgtgc
    14401 ggggtatcgc ttgctgaaga atgccggttg cttaccgcca gaacttgagc aacggagaga
    14461 agcaattcag cttctggata ttctcaaagg tatccgtcac gatgatccgc aatatcaaga
    14521 ggttagccgt cgattgtcat tactggaatt gaagctgcga caagctggat tgagtaccga
    14581 ttttttacgc ggcgattatg ctgacaagtt gttggacaaa atcaacgata actagtggag
    14641 tatgtatgta tcgcattggt gagctggcaa aaatggcgga agtaacacct gacacgattc
    14701 gttattacga aaaacagcag atgatggagc atgaagtgcg tactgaaggc gggtttcgcc
    14761 tgtataccga aagcgatctc cagcgattga aatttatccg ccatgccaga caactaggtt
    14821 tcagtctgga gtcgatccgc gagttgctgt cgatccgcat cgatcctgaa caccatacct
    14881 gtcaggagtc aaaaggcatt gtgcaggaaa gattgcagga agtcgaagca cggatagccg
    14941 agttgcagag tatgcagcgt tccttgcaac gccttaacga tgcctgttgt gggaccgccc
    15001 atagcagtgt ttattgctcg attcttgaag ctcttgaaca aggggcgagt ggcgttaaga
    15061 gtggctgttg attttttgca ctgacaggat tacactcgcg ccgttaaata accaactgga
    15121 gtttttatga gccgatatca gcatactaaa gggcagataa aggataatgc gatagaagca
    15181 ttactacatg atcccttatt ccgacagcga gtagagaaaa ataagaaagg gaaaggcagt
    15241 tacatgcgaa aaggaaaaca tggcaatcgg gggaactggg aggccagtgg caagaaagta
    15301 aatcactttt ttaccactgg tcttctgctt tcaggcgctt gttaagagcg gttattctgc
    15361 tctttcagca aatcacgaat ttctgccagt aatacttctt ctttagttgg tgcaggtgcg
    15421 gcagctggtt cttctttttt ccgattcagt ttgttgatta gcttaatcgc cataaagatg
    15481 gcaaaggcca caatcagaaa atcaaagacg ttttgaatga agacaccgta atgcatcaca
    15541 acagcaggga tatccccctg cgcatcgcgt agcgtgacag caaactgttt aaagtcgatc
    15601 ccgccaatta ataagcccag aggtggcatg atgatatcgg caaccagtga agatacaatc
    15661 ttcccgaatg ccgcaccgat aatgacaccc accgccaaat ccaccacgtt cccgcgcatc
    15721 gcaaattcgc gaaattcttt aataatgctc atgttattct ccctatgaag ccgacaacca
    15781 taagtctaac aaatgttaag ccattttcct gccaggaatg aatttaatta atccttgata
    15841 atcaaaggga aataataagg cgtcattaga cgccttatta attacaagaa gaaagggctt
    15901 ggctggaaga gtctttcgac gtcggtaata aactttttat ctgtgaggaa cataattacg
    15961 tgatcgcctt gctcaatgcg cagattgtca ttggcaatca tcacgtcgtt tccacgtacc
    16021 accgctccaa taatcgttcc tggcggtagc ttgatttcgt caatgactct gccgacaacg
    16081 cgtgaggtgc tttcatcacc gtgagcaacg gcttcaatag cttctgctac gccgcggcgc
    16141 aatgaggaaa caccaacaat atctgctttt cgcacatggc taagcaacgc agaaatagtt
    16201 gcttgttgtg gtgaaattgc aatatcgatc acgctcccct gaaccagatc cacataagcg
    16261 cgacgctgga tcaataccat cacctttttc gcacccatac gtttggcaag catggcggac
    16321 atgatattgg cctcgtcatc gttggtgacg gcaataaaca gatcaacttg atcgatatgt
    16381 tcttcggcca gcagttcttg atccgacgca tcaccaaaaa agacgatcgt attctgcaac
    16441 ttttccgcca gttcggcagc gcgctgctga ttacgttcga tgagtttaac gctgtaatct
    16501 ttttccagac gacgcgccag ccctgcaccg atattaccgc caccaaccag catgatccgc
    16561 ttatacggtt tttccagtcg ctgtaattca ctcatcaccg cgcggatatg ctgtgaagcg
    16621 gcaataaaga acacttcatc accagcttca acaatggtcg aaccttgtgg acgaatgggg
    16681 cgatcgtggc ggaaaattgc tgccacacga gtatcgatat gtggcatatg ttcgcgcatg
    16741 gtcgaaagtg cattaccaat cagcgggccg ccataatagg ctttaactac agcgaggctg
    16801 actttaccct cagcgaagtt caccacctgc aatgcgcctg gatactcaat cagtcggtaa
    16861 atattatcga taaccaactg ctctggtgcg atcagatgat caatcggtac agcatctgaa
    16921 tgaaatagct tatcggcatc gcgtacgtag tctggtgagc ggatgcgagc gatgcgatta
    16981 ggggtgttga aaagtgagta ggctacctgg caggcaacca tattggtttc atctgaactg
    17041 gttacagcaa ccagcatatc ggcgtcgtcg gcacctgcct cccgcaatac gcgtggatga
    17101 gagccatgcc cctgcacgac tcgcaggtca aatttatcct gcaaggtccg cagacgctca
    17161 ccgttggtat cgacaacagt aatatcgttg ttctcgccaa ccaggttttc cgccagtgtg
    17221 ccgccaacct ggccggcacc cagaatgata attttcatca gtctcgaccc gttatctcat
    17281 cactttttga ttagcttagc gtaaaagaag ccgtcgccct cttcggcacc aggcagattt
    17341 tgtttacccg gttgctctgg tgttcctgtt tcgcaaagtt cggcatcagc ggtacgttgc
    17401 aaaaaagctt taatctgcag gctattctct tccggtaaca ccgaacaggt ggcatagacc
    17461 agggttccac cggattttaa atgcggccaa atggcgtcga gaatttcaga ctgcaattgc
    17521 gcgagttccg ggatatcgcg atcgcgacgt aaccatttaa tatccggatg gcgacgaatc
    17581 acccccgttg ctgaacaagg cgcatctaat aaaatgcgat caaactgttg ctcgccacac
    17641 cattgggaag ggtaacggcc atcaccttgt ttcacggtcg ccttcatacc aaggcgtttt
    17701 aaattgtcgt gaacgcgaga gaggcgctgt tcgtcaatat caaccgcaac aacctgcgct
    17761 tctggtgcca cctcaaggat atgcgttgtt ttaccgccgg gggcggcaca aagatccaga
    17821 atgtgttcac cgttttgtgg cgcaagccag gtcatgcaac cttgtgctga tgcatcctga
    17881 acggtaaccc atccgtcttc aaaaccaggt agcgcatgaa caggtgcagg tgtttccagc
    17941 cgtacagcat cagggtaatc cgcatgcggg aaacctttca ttcctgcttc atccaacaat
    18001 gcaagccagc tgtcgcggga atgatgcgta cgattaacgc gcagccacat tggcggacgc
    18061 tggttattgg cttcgacgat ggattgccac tgctctggat acgctttttg cagacgcttc
    18121 agcaaccagg aaggatgcag ataacgtgca tcactggtat taaactcggc taataactct
    18181 tcttgctgat gctggaactg gcgtaataca ccgttaatca accctttaag ttgcggacgc
    18241 ttaattgcga tagcgccttc aaccgtttca gccagcgcag catgaggtgg aatgcgggta
    18301 taaagcagtt gatacaaacc aaccataatc aggtaatgca cagtgcgctg tttgccggtc
    18361 atcggacggg ccattaactt attaatcagc cagtctaact gcgaaagcgt acgcagtacg
    18421 ccaaagcaca actcttgaag aagtgctttg tctttatcag aaactttttg ctggagcggt
    18481 ggcagaatgt tgcttaatga ttgcccttgc tcgacgactt gttcaacggc ctgggccgcc
    18541 atgctacgta aattacgttg ttttttcata agtataaaaa tgcccggtaa gaccgggctt
    18601 agaagagtgg actatcaggc cagacggttg cccggaacaa accattcccg acgagaattc
    18661 aggaggtctt gcgcgctcat cgctttctta cccgcaggtt gtaacgagag caggttcagg
    18721 atgccatcac cagtcgcaac ctgaatgcct tgtttgttgg cttcaaggat cgttcctggt
    18781 gcagcgttgg ttgccgtatc aatgaccgac gctttccaga ctttaaccgg ttgtccttca
    18841 atttccagcc agctcattgg ccatgggttg aaagcgcgaa tgcagcgttc aagctgcgct
    18901 gccgaaagcg accagtcaat acgcgcttct tctttactca acttctcggc gtaagtgaca
    18961 agagtttcgt cctgaacttc tggtttcgcc gtgccgtctg ctagttgttt caacgtggtg
    19021 attagccctt gtgggccaag ctctgccagc ttgtcgtaca gcgtaccact ggtatcttct
    19081 gcagtaatcg ggcaggagag cttatagagc atatcaccgg tgtctaaacc gacatccatt
    19141 tgcataatgg tcacaccagt ttctgcatca cccgcccata gtgagcgttg gattggtgca
    19201 gcaccgcgcc agcgtggcag cagtgaacca tgaacgttga tacagccaag acgcggcatc
    19261 tccagcactg ctttaggcag aattaagcca taggcgacga cgaccataac atcagcctgc
    19321 agatcggcga ccagttgctg gctttcttgt ggacgcaggg aaacaggttg aaaaacgggc
    19381 agaccttttt cctcagccag aactttaacc gggctgggca tcagtttttt accgcgtcct
    19441 gccggtcggt ctggctgggt gaacacgcca acgacgttat gaccagaaga caacagcgcg
    19501 tcgagatgac gcgctgcaaa gtcaggtgta cccgcaaaaa taatacgtag tgattctgac
    19561 acgttagttc ttatccttaa gcccgggctt tcagacgatc tagtttttca actttctgac
    19621 gaatacgttg ttgtttcagc ggtgacagat aatccataaa cagtttgccg accaggtgat
    19681 ccatctcatg ctgaatacag atggctaaca gcccctctgc ttccagttca aatggtttac
    19741 cgtcgcggtc aagggcgcga attttaactt tctctgcgcg cggcactaaa gcacgttgtt
    19801 cagggatcga caggcaacct tcttcaatgc ctgtttcgcc gcttttttct aaaagctccg
    19861 gattgattaa caccagccgt tcgtcacggt tttccgaaac atcaataaca atgatacgtt
    19921 gatggatatc aacctgggtt gccgccaggc caataccttc ttctgcgtac atcgtctcga
    19981 acatatcatc gacgatacgc tgtatttctg cattcacttc ttctaccggt ttagcaactt
    20041 tgcgaagccg ctcgtccgga atatgtaaca cttgcaaaac tgacataaat ctccagagat
    20101 gtgttcaggg gttagaaaga ttatttcttc tattctagac aaatccccct ctgattgaca
    20161 gcatcactga ccaatcgcaa agattgctaa ggctgcttat ggcagggaga taaggatggt
    20221 cgatacagaa atttggctgc gtttaatgag tatcagcagc ttgtacggcg atgatatggt
    20281 ccgtatagct cactggctgg caaaacagtc gcaaattgat gcggttggat tgcagcaaac
    20341 agggcttaca ttgcggcagg cacaacgctt tctttcattt ccgcgaaaga gtatcgaaag
    20401 ctcactttgt tggttggagc aacccaacca tcatttaatc cctgcggaca gcgaatttta
    20461 tcctcctcaa cttctggtga cgacagatta ccccggcgca ctgtttgttg aaggagaact
    20521 gcacgcgctg cattcatttc agcttgccgt agtggggagt cgggcgcatt catggtatgg
    20581 cgagcgatgg ggacggttat tttgcgaaac tctggcgacg cgtggagtga caattacgag
    20641 tggactggcg cgtggaatcg atggtgtggc gcataaagct gccttacagg taaatggcgt
    20701 cagcattgct gtattgggga atggacttaa taccattcat ccccgccgcc atgcccgact
    20761 ggctgccagt ctgcttgaac atggcggagc tctcgtctcg gaatttcccc tcgatgttcc
    20821 accccttgct tacaatttcc cacgaagaaa tcgcattatc agtggtctaa gtaaaggtgt
    20881 actggtggtg gaagcggctt tgcgcagtgg ttcgctggtg acagcacgtt gtgcgcttga
    20941 gcagaggcgt gaagtttttg ccttgccagg tccaataggg aatccgggaa gcgaagggcc
    21001 tcactggtta ataaaacaag gtgcgattct tgtgacggaa ccggaagaaa ttctggaaaa
    21061 cttgcaattt ggattgcact ggttgccaga cgcccctgaa aattcatttt attcaccaga
    21121 tcagcaagac gtggcattgc catttcctga gctcctggct aacgtaggag atgaggtaac
    21181 acctgttgac gtcgtcgctg aacgtgccgg ccaacctgtg ccagaggtag ttactcaact
    21241 actcgaactg gagttagcag gatggatcgc agctgtaccc ggcggctatg tccgattgag
    21301 gagggcatgc catgttcgac gtactaatgt atttgtttga aacctatatt cacactgaag
    21361 ctgagttgcg tgtggatcaa gacaaacttg aacaggatct taccgacgca ggttttgagc
    21421 gagaagatat ctacaatgcc ctgctatggc tggaaaaact tgctgattat caggaagggt
    21481 tggcagaacc gatgcaactg gcctcggatc ctctctccat gcgtatttat acaccggaag
    21541 agtgtgaacg actggatgcc agctgccgtg ggtttctgct tttccttgag cagattcagg
    21601 tgctcaacct tgaaactcgt gaaatggtga tagagcgagt gctcgcgctg gataacgcag
    21661 agttcgaact ggatgatctg aaatgggtga tcctgatggt gttgttcaac atcccggggt
    21721 gcgaaaatgc gtaccagcaa atggaagaat tactctttga agtgaatgaa ggtatgctgc
    21781 attaatcatt tctttaattc agcataagtt gttatggcaa aatcagcact gttcacggtg
    21841 cgtaataatg agtcctgccc aaagtgcggg gctgaactgg ttattcgatc cgggaaacac
    21901 ggtccgtttc ttggatgctc acagtatccg gcgtgtgact acgtccgtct tctgaaatct
    21961 tcagcggatg gacatattgt caaagttctg gaggggcagg tttgccctgc atgtggcgca
    22021 aatctggtat tacgccaggg acgctttggt atgtttattg gttgcagtaa ctaccctgaa
    22081 tgcgaacata ccgaacttat cgataaaccg gacgaaacag caattacatg cccccaatgt
    22141 cggacgggcc atctggtcca gcgccgctcc cgttatggca aaacatttca ctcctgtgat
    22201 cgctacccgg agtgtcaatt tgccattaac ttcaaaccaa tagctggaga atgccctgag
    22261 tgtcattatc cgctactcat cgaaaagaaa accgcgcagg gtgtaaaaca cttttgtgcc
    22321 agtaaacaat gtggaaagcc ggtttcggcg gaataataac gtgaataata acctgcaagg
    22381 agacgctatc gcagctgcga tagatgtcct caatgaagaa cgtgtcatcg cctatccaac
    22441 ggaagccgtt ttcggtgttg ggtgcgatcc tgatagcgaa acagcagtga tgagactgtt
    22501 ggagttaaaa cagcgtccgg ttgataaggg gctgatttta atcgcggcaa attacgagca
    22561 gcttaaaccc tatattgatg acaccatgct gactgatgcg cagcgtgaaa ccattttttc
    22621 ccgctggcca ggtcctgtca cctttgtctt tcccgcgcct gcgacaacac cgcgctggtt
    22681 gacgggccgc tttgattcgc ttgctgtacg agtcaccgac catccgttgg tggttgcttt
    22741 gtgccaggct tatggtaaac cgctggtttc taccagtgcc aacttgagtg gattgccacc
    22801 ttgtcgaaca gtagacgaag ttcgcgcaca atttggcgcg gcgttcccgg ttgtgcctgg
    22861 tgaaacgggg gggcgtttaa atccttcaga aatccgcgat gccctgacgg gtgaactgtt
    22921 tcgacagggg taacataatg gaaacctatg ctgtttttgg taatccgata gcccacagca
    22981 aatcgccatt cattcatcag caatttgctc agcaactgaa tattgaacat ccctatgggc
    23041 gcgtgttggc acccatcaat gatttcatca acacactgaa cgctttcttt cgtgctggtg
    23101 gtaaaggtgc gaatgtgacg gtgcctttta aagaagaggc ttttgccaga gcggatgagc
    23161 ttactgaacg ggcagcgttg gctggtgcag ttaataccct caagcggtta gaagatggac
    23221 gcctgctggg tgacaatacc gatggtgtag gcttgttaag cgatctggaa cgtctgtctt
    23281 ttatccgccc tggtttacgt attctgctta tcggcgctgg tggagcatct cgcggcgtac
    23341 tactgccact tctttcccta gactgtgcgg tgacaatcac taatcggacg gtatcccgcg
    23401 cggaagagtt ggctaaattg tttgcgcaca ctggcagtat tcaggcgttg ggtatggacg
    23461 aactggaagg tcatgagttt gatctcatta ttaatgcaac atccagtggc atcagtggtg
    23521 atattccggc gatcccgtca tcgctcattc atccaggcat ttattgctat gacatgttct
    23581 atcagaaagg aaaaactcct tttctggcat ggtgtgagca gcgaggctca aagcgtaatg
    23641 ctgatggttt aggaatgctg gtggcacagg cggctcatgc cttccttctt tggcatggcg
    23701 ttctgcctga tgtagaaccg gtcatcaaac tattgcaaca ggaattgtca gcgtgaatca
    23761 ggccatccag tttccggata gggaagagtg ggacgagaat aaaagatgtg tatgttttcc
    23821 tgctctcgtg aatggtatgc aactgacatg tgcgatctct ggcgagagtc tggcgtatcg
    23881 ctttactgga ggtacgccag aacagtggtt agcgagtttt cgtcagcatc gctgggacct
    23941 ggaagaagaa gcggaaaact taattcagga acaaagtgaa gatgatcaag gttgggtctg
    24001 gttaccttga tccagatatt cgtccttcca tttcacgtag ttatttgcgg aatagcgtaa
    24061 cccagccttc tcttcatcac ttaacgggcg gatctgtttg acgggactac cgagatacag
    24121 atatccgctc tccagccgtt tattttgtgg gaccaggcta cccgcaccaa tcatcacatc
    24181 atcttctact attgcgccat caagtaaaat tgagcccatc ccaaccaaaa ctcgattgcc
    24241 aatggtgcag ccgtggagca tcaccttgtg accaacagtg acatcttcgc caatggttaa
    24301 tgggttgcca tctgggttgt acgaggattt atgagtgaca tgcaacatac tgccatcctg
    24361 gatattggtg cgtgctccga tctgtacata atgtacatct ccacgaatca caacgagcgg
    24421 ccagatcccc acatcatcag ccagacgaac gtcaccaatc acgacgctgc tatcgtcgat
    24481 cattacgcgc tggccgattt gtggaaaaag atcgcggtat gggcgtaaaa catcagacat
    24541 actcacctca acaataaatg atttcctaat gactttgggt gcattattag ccttgtgcaa
    24601 gtcttttagt atgcaaaaaa gcaccgtttt gtgtgcgaat gcagcaaaaa gggtgaaaaa
    24661 acaacaaaca gaaaaaaaga tcaaaaaagt acttgtgcaa aaaattggga tccctataat
    24721 gcgcctccgt tgagacgaca acgtgaaaca cttcacagga tggtcggaac aacgaagaga
    24781 aaaaattctg aaattcaggg ttgactctga aagaggaaag cgtaatatac gccacctcgc
    24841 gacagtgagc tgaaagccgc gtcgcaactg ctctttaaca atttatcaga caatctgtgt
    24901 gggcactcga agatacggat tcttaacgtc gcaagacgaa aaatgaatac caagtctcaa
    24961 gagtgaacac gtaattcatt acgaagttta attctttgag catcaaactt ttaaattgaa
    25021 gagtttgatc atggctcaga ttgaacgctg gcggcaggcc taacacatgc aagtcgaacg
    25081 gtaacaggaa gcagcttgct gcttcgctga cgagtggcgg acgggtgagt aatgtctggg
    25141 aaactgcctg atggaggggg ataactactg gaaacggtag ctaataccgc ataatgtcgc
    25201 aagaccaaag agggggacct tcgggcctct tgccatcgga tgtgcccaga tgggattagc
    25261 ttgttggtgg ggtaacggct cacc
//
LOCUS       DAESHI010000054        25106 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.054, whole genome shotgun sequence.
ACCESSION   DAESHI010000054 DAESHI010000000
VERSION     DAESHI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25106
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.054"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            196..384
                     /locus_tag="KW011_002313"
     CDS             196..384
                     /locus_tag="KW011_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HBH7660627.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            396..1148
                     /gene="bcsQ"
                     /locus_tag="KW011_002314"
     CDS             396..1148
                     /gene="bcsQ"
                     /locus_tag="KW011_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HBH7660628.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1145..1309
                     /locus_tag="KW011_002315"
     CDS             1145..1309
                     /locus_tag="KW011_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HBH7660629.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1502..3763
                     /gene="bcsA"
                     /locus_tag="KW011_002316"
     CDS             1502..3763
                     /gene="bcsA"
                     /locus_tag="KW011_002316"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HBH7660630.1"
                     /translation="MNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSLILA
                     LICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRYTST
                     LNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVDIFV
                     PTYNENLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIARTTHE
                     HAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHFFSP
                     DPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSYAVIRRKPLDEIGGIAVET
                     VTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLDNPL
                     TGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFVLPH
                     MIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGGLVE
                     EEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVLGGA
                     VAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQILEGQ
                     KVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWALWQD
                     SYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRHPERS
                     ETAQPSDQALAQQ"
     gene            3774..6113
                     /gene="bcsB"
                     /locus_tag="KW011_002317"
     CDS             3774..6113
                     /gene="bcsB"
                     /locus_tag="KW011_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBH7660631.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKSDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVLRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6120..7226
                     /gene="bcsZ"
                     /locus_tag="KW011_002318"
     CDS             6120..7226
                     /gene="bcsZ"
                     /locus_tag="KW011_002318"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HBH7660632.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7208..10700
                     /gene="bcsC"
                     /locus_tag="KW011_002319"
                     /pseudo
     CDS             7208..10700
                     /gene="bcsC"
                     /locus_tag="KW011_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10782..12770
                     /gene="hmsP"
                     /locus_tag="KW011_002320"
     CDS             10782..12770
                     /gene="hmsP"
                     /locus_tag="KW011_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HBH7660633.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12953..14239
                     /gene="dctA"
                     /locus_tag="KW011_002321"
     CDS             12953..14239
                     /gene="dctA"
                     /locus_tag="KW011_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HBH7660634.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14459..15955
                     /locus_tag="KW011_002322"
     CDS             14459..15955
                     /locus_tag="KW011_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBH7660635.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16051..16980)
                     /gene="kdgK"
                     /locus_tag="KW011_002323"
     CDS             complement(16051..16980)
                     /gene="kdgK"
                     /locus_tag="KW011_002323"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HBH7660636.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17212..17979
                     /gene="pdeH"
                     /locus_tag="KW011_002324"
     CDS             17212..17979
                     /gene="pdeH"
                     /locus_tag="KW011_002324"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HBH7660637.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18049..20109
                     /locus_tag="KW011_002325"
     CDS             18049..20109
                     /locus_tag="KW011_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBH7660638.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20291..21613)
                     /locus_tag="KW011_002326"
     CDS             complement(20291..21613)
                     /locus_tag="KW011_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HBH7660639.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22024..23037)
                     /gene="yhjD"
                     /locus_tag="KW011_002327"
     CDS             complement(22024..23037)
                     /gene="yhjD"
                     /locus_tag="KW011_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HBH7660640.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23086..23985)
                     /locus_tag="KW011_002328"
     CDS             complement(23086..23985)
                     /locus_tag="KW011_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH7660641.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24505..>25106
                     /locus_tag="KW011_002329"
     CDS             24505..>25106
                     /locus_tag="KW011_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH7660642.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5894 a         6365 c         6992 g         5855 t
ORIGIN      
        1 atttagcttg tgaatcaatt tagaaggtca caagacgtgt gattttagtc ttttcgtttt
       61 taactttttc tgttgcgtaa cggaaagtca aaaagtgagc aaattcccgt ctcgccgtaa
      121 acaataaacc ggcgaaatgc tcacggttag aattgtttca tgaacggtac ggttatttca
      181 tagggatcaa gcaaaatgaa taacaatgaa ccagatactc tgcctgatcc cgcgataggc
      241 tatatcttcc agaatgatat tgtggcgtta aagcaggcat tttcactgcc tgatattgat
      301 tatgccgata tttcccaacg cgaacagttg gccgcggcat taaaacgctg gccgttgctg
      361 gcagagtttg cgcaacaaaa gtaggggatt ggtgaatggc cgtactggga ttgcaggggg
      421 tgcggggagg cgtggggaca acaaccatca ccgccgcatt agcctggtca ttacaaatgt
      481 tgggagaaaa tgtcctggtg gtcgatgcct gcccggacaa cttgttgcgc ctgtcattta
      541 atgttgattt tacccaccgt cagggctggg ccagagcgat gctggatggc caggactggc
      601 gtgacgctgg gttgcgctac acctcgcagc tcgatttgct gccttttggt cagttatcca
      661 ttgaagaaca agaaaatcca cagcactggc aaacccggct gagcgatatt tgctccggct
      721 tacagcaact aaaagccagc gggcgttacc agtggatttt aatcgactta ccgcgtgatg
      781 cctcgcagat aacccaccag ctgctgagtt tgtgcgatca ctcgctggca atcgtcaatg
      841 tggatgccaa ctgccatatc cgactgcatc agcaagcgct gccggatggc gcacatattt
      901 tgattaatga cttccgtatt ggcagtcagg ttcaggacga tatttaccag ctttggttgc
      961 aaagccagcg ccgattactg ccgatgctca ttcatcgtga tgaagcgatg gctgaatgcc
     1021 tggcggctaa gcaaccagta ggtgaatatc gcagtgatgc gctggcggct gaagagatac
     1081 tgacgctggc gaactggtgc ctgttgaact actccgggct gaaaacgcca gtcgggagtg
     1141 catcatgagt atcctgaccc ggtggttgct tatcccgccg gtcaacgcgc ggcttatcgg
     1201 gcgttatcgc gattatcgtc gtcacggtgc gtcggctttc agcgcgacgc tcggctgttt
     1261 ctggatgatc ctggcctgga tttttattcc gctggagcac ccgcgctagc agcgtattcg
     1321 cgcagaacat aaaaacctgt atccgcatat caacgcctcg cgtccgcgtc cgctggaccc
     1381 ggtccgttat ctcattcaaa catgctggtt actgatcggt acatcgcgca aagaaacgcc
     1441 gaaaccgcgc aggcgggcat tttcaggtct gcagaatatt cgtggacgtt accatcaata
     1501 gatgaacgag ctgcctgagc gcgttagcca taaaacacag catcttgatg agaaaaaaga
     1561 gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg tcaccttctc
     1621 gctgattctg gcgttaatct gcgttactca accgtttaac ccgctggcgc agtttatctt
     1681 cctgatgctg ctgtggggtg tagcgctgat cgtacggcgg atgccggggc gcttctcggc
     1741 gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt ggcgttacac
     1801 ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc tgctcttcgc
     1861 tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat ggccgctgaa
     1921 tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg atatctttgt
     1981 cccgacttac aacgaaaatc tcaatgtggt gaaaaatacc atttacgcct cgctgggtat
     2041 cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca gggaagagtt
     2101 tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc atgaacatgc
     2161 gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg tgtcgatttt
     2221 cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct ggttcctgaa
     2281 agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg acccgtttga
     2341 acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct atggtctggt
     2401 gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctatg cggtgattcg
     2461 tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg aagatgcgca
     2521 tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta ttccgcaggc
     2581 ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc gctgggcgcg
     2641 cgggatggta caaatcttcc gtctcgataa cccgctcacc ggtaaaggac tgaagtttgc
     2701 tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc cacggctgat
     2761 cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct atgcgccagc
     2821 gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga ccaactcgaa
     2881 gatccagggc aaatatcgcc actctttttg gagtgaaatc tacgaaacgg tgctggcgtg
     2941 gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca aatttaacgt
     3001 caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct cgcggcccta
     3061 catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc gctacttcta
     3121 tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt tctacaacct
     3181 gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac gccgatcgca
     3241 ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc tcttctcgtg
     3301 taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc aggcgcagat
     3361 tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat acgtcttccc
     3421 gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc cgctcaccac
     3481 ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat gggcgctctg
     3541 gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc tgaagctcgg
     3601 cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg gcatattccg
     3661 tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccacc cggagcggag
     3721 cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac gcgatgaaaa
     3781 gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc tctttcatga
     3841 cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta gccgcccaga
     3901 cggaacaaaa tccgcaggtg gggcaagtga tgccgggcgt gcagggcgct gatgcgccag
     3961 tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa attgcgccgc
     4021 cgccgggcag catggtgcta cgtggcatta acccgaacgg cagcattgag tttggtatgc
     4081 gcagcgatga agtggtgacg aaggcgatgc tcaacctcga atacacgcca tcgccatcgt
     4141 tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg ggcgtgctgc
     4201 cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt aacccactgt
     4261 ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag gacgtgtgcg
     4321 aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga ctggatctga
     4381 cctatcagac cctgaatgtg aagagtgacc tgtcacactt cccggtgcca ttctttgacc
     4441 cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg gatgttgggc
     4501 tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc tggcgtgggc
     4561 agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc tttgcaacca
     4621 acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg gtgattgaga
     4681 tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt cgtgacgaca
     4741 aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc cgtggtgaaa
     4801 gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat gcgccgaact
     4861 gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa gaacagttac
     4921 aatccagcgg tcttgagcca gcagcgatta acgtttcgct aaacctgccg ccggatctct
     4981 acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc atgccgccgg
     5041 tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa tccttcaacc
     5101 tgagcagtaa acaggaggcg aaccgcctgc tgctgcggat tccggtatta caaggtttgc
     5161 tggatggcaa aacggatgtc tctattccgg cgctgaaact gggcgcgacc aaccagctgc
     5221 gcttcgactt tgagtatatg aacccgatgc cgggcggttc ggtggataac tgtattacct
     5281 tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac ttctcgaagt
     5341 attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc ttcccgttca
     5401 gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaaacgcct aacgaagcac
     5461 agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc ttcccggcga
     5521 ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc gacatcatga
     5581 tcatcggtgg tatcccggac aaattaaaag acgataagca gatcgacctg ttggtgcagg
     5641 cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc attgtgccgg
     5701 acgagagcga tcgcgcggca gaaacccagt caacgctgac ctcttccggt gcgatggcgg
     5761 cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg ctgctggcag
     5821 atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc aaacgcgcca
     5881 ccatgttcgg ttcggtcgcg gtgctccgcg agtccggtat caacagccta cgtgttggcg
     5941 acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg ctggcaaacc
     6001 atccgattct gctggcggtg ctggcggcaa tcagtgtgat attgctggca tgggtactgt
     6061 ggcgtctgct gcgaattatt agtcgtcgtc gtcttaaccc ggataacgag taattgaaga
     6121 tgaatgtgtt gcgtagtgga atcgtgacga tgctgctgtt ggctgccttt agtgttcagg
     6181 cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt caggaagggc
     6241 gcgtcatcgc ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa agttacggta
     6301 tgttctttgc cctggcggct aacgaccgtg tagctttcga taatattctc gactggacgc
     6361 agaacaatct cgctcagggc tctttaaaag aacgtttgcc cgcctggctg tggggcaaga
     6421 aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt gatgtctgga
     6481 tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat accgacatcg
     6541 gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct gggctgggtt
     6601 ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt tttaacccca
     6661 gctacctgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg tggaccacgc
     6721 tgcgcgaaac caatcaacgt ttattgctgg aaaccgcccc gaaaggtttt tcgccagact
     6781 gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca ttgatcagca
     6841 gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc gatccgcaaa
     6901 aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa aacggttatc
     6961 cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg atcggttttt
     7021 ctgccgccat gctgcccttt ttacaaaacc gcgatgcgca ggccgttcag cgccagcgcg
     7081 tggccgataa ctttcccggc agcgatgcct attacaacta tgtgctgacc ctgtttggac
     7141 aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta cctgactggg
     7201 gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc tggccgtcat
     7261 gccgatggtc gaggcagcac caaccgctca gcaacagttg ctggagcaag ttcggttagg
     7321 cgaagcgacc catcgtgaag atctggtgca acagtcgtta tatcggctgg aacttattga
     7381 tccgaataac ccggacgtcg ttgccgcccg tttccgttct ttgttacgtc agggcgatat
     7441 tgatggcgcg caaaaacagc tcgatcggct gtcgcagtta gcgccgagtt caaatgcgta
     7501 taaatcgtcg cggactacga tgctactttc cacgccggat ggtcgtcagg cactgcaaca
     7561 ggcacgattg caggcgacga ccggtcatgc agaagaagct gtggcgagtt acaacaaact
     7621 attcaacggt gcgccgccgg aaggtgacat tgctgtcgag tactggagta cggtggcgaa
     7681 aattccggct cgccgtggcg aagcgattaa tcagttaaaa cgcatcaatg cggatgcacc
     7741 gggcaatacg ggcctgcaaa acaatctggc gctattgctg tttagtagcg atcgccgtga
     7801 cgaaggtttt gccgtcctgg aacagatggc aaaatcgaac gccgggcgcg aaggggcctc
     7861 taaaatctgg tacgggcaga ttaaagacat gcccgtcagt gatgccagtg tgtcggcgct
     7921 gaaaaaatat ctctcgatct ttagtgatgg cgatagcgtg gcggctgcgc aatcgcaact
     7981 ggcagaacag caaaaacagc tggccgatcc tgctttccgc gctcgtgcgc aaggtttagc
     8041 ggcggtggac tctggtatgg cgggtaaagc cattcccgaa ctacaacagg cggtgcgggc
     8101 gaacccgaaa gacagtgaag ctctgggggc gctgggccag gcgtattctc agaaaggcga
     8161 tcgcgccaat gcagtggcga atctggaaaa agccctcgca ctggacccgc acagcagcaa
     8221 caacgacaaa tggaacagtc tgctgaaagt aaaccgctac tggctggcga tccagcaggg
     8281 cgatgctgcg ctgaaagcca ataatcctga ccgggcagaa cgcctgttcc agcaggcgcg
     8341 taatgtcgat aacaccgaca gttatgcagt gctggggctg ggcgatgtgg cgatggcgcg
     8401 aaaagattat cccgccgccg aacgttatta tcagcagacc ttgcgtatgg acagcggcaa
     8461 cactaacgcc gtgcgcgggc tggcaaatat ttaccgccag caatcgccag aaaaagctga
     8521 agcgtttatc gcctcgctct ctgccagtca gcggcgtagc attgatgata tcgaacgcag
     8581 cctgcaaaac gaccgtctgg cacagcaggc agaggcactg gaaaaccagg gcaaatgggc
     8641 gcaggcggca gcacttcagc ggcaacgact ggcgctggac cccggcagcg tatggattac
     8701 ttaccgactt tcgcaggatc tctggcaggc cggacaacgc agccaggccg atacgttaat
     8761 gcgcaatctg gcgcagcaga agtcgaacga cccagagcag gtttacgctt acgggctgta
     8821 cctacgctta cgggctgtac ctctctggtc atgaccagga cagagcggcg ctggcgcata
     8881 tcaacagcct gccgcgtgcg cagtggaaca gcaatattca ggagctggtt aatcgactgc
     8941 aaagcgatca ggtgctggaa accgctaacc gcctgcgaga aagcggcaaa gaggcagaag
     9001 cggaagcgat gctgcgccag caaccacctt ccacgcgtat tgacctcacg ctggctgact
     9061 gggcgcaaca acgacgtgat tacaccgccg cccgcgctgc atatcagaat gtcctgacgc
     9121 gggagccagc taacgccgac gccattcttg gtctgacgga agtggatatt gctgccggtg
     9181 acaaagcggc ggcacgtagc cagctggcga aactgcccgc taccgataac gcctcgctga
     9241 acacacagcg gcgcgtggcg ctggcacagg cgcagcttgg cgataccgca gcagcgcagc
     9301 ggacgtttaa taagttgatc ccgcaggcaa aatctcagcc accgtcgatg gaaagcgcga
     9361 tggtgctgcg tgatggtgcg aagtttgaag cgcaggcggg cgatccaacg caggcgctgg
     9421 aaacctacaa agacgccatg gtcgcatccg gtgtgactac gacgcgtccg caggataacg
     9481 acacctttac ccgactgacc cgtaacgacg agaaagatga ctggctgaaa cgtggcgtgc
     9541 gcagcgatgc ggcggacctc tatcgccagc aggatcttaa cgtcaccctt gagcacgatt
     9601 actggggttc gagcggcacc ggtggttact ccgatctgaa agcgcacact accatgttgc
     9661 aggtggatgc gccgtattct gacgggcgga tgttctttcg cagtgatttc gtcaatatga
     9721 acgtcggcag tttctccact aatgccgatg gcaaatggga tgacaactgg ggcacctgta
     9781 cattacagga ctgtagcggc aaccgcagcc agtcggattc cggtgccagc gtggcggtcg
     9841 gctggcgaaa tgacgtctgg agctgggata tcggtaccac gccgatgggc ttcaacgtgg
     9901 tggatgtggt cggcggcatc agttacagcg atgatatcgg gccgctgggt tacaccgtta
     9961 acgcccaccg tcggcccatc tccagttctt tgctggcctt tggtgggcaa aaagactccc
    10021 cgagcaatac cgggaaaaaa tggggtggcg tacgtgccga cggtgtgggg ctaagtctga
    10081 gctacgataa aggtgaagca aacggcgtct gggcatcgct tagtggcgac cagttaaccg
    10141 gtaaaaatgt cgaagataac tggcgcgtgc gctggatgac gggctattac tataaggtca
    10201 ttaaccagaa caatcgccgc gtcacaatcg gcctgaacaa catgatctgg cattacgaca
    10261 aagatctgag tggctactca ctcggtcagg gcggttacta cagtccgcag gaatacctgt
    10321 cgtttgccat accggtgatg tggcgggagc gcacggaaaa ctggtcgtgg gagctggggg
    10381 cgtctggctc gtggtcgcat tcacgcacca aaaccatgcc gcgttatccg ctgatgaatc
    10441 tgatcccgac cgactggcag gaagaagctg cgcggcaatc caacgatggc ggcagcagtc
    10501 agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc aactggtttg
    10561 ttggcacggc aattgatatc cagcaggcga aagattacgc acccagccat ttcctgctct
    10621 acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg cagccgctga
    10681 taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc tgcgatcggg
    10741 tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt
    10801 taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt tttatttttt
    10861 gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct acgcaactgg
    10921 aaagcattgc tcgctctgtc cgcgaaccct tatcttcagc tattttgaaa ggcgatattc
    10981 ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc cgtgccgatg
    11041 tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag cgcccggtgc
    11101 cgataatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc gtttactcgc
    11161 tcgaacgtcc ggcaaacccg cagccaattg cctatctggt actacaggcg gattccttcc
    11221 gtatgtataa gttcgtgatg agcaccctct caacgttagt gaccatttac ttacttttgt
    11281 cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg catccgttac
    11341 gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt caccaactgg
    11401 cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca
    11461 accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg cgcttcccgg
    11521 tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt gtcgcgcgta
    11581 aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc
    11641 tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc aaatcggtac
    11701 tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc attgccaacg
    11761 gtgtacagga accgtggcac gcaatcacct taggtcagca agtgctcact atcatgagcg
    11821 agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc gtggcgatgt
    11881 tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg gcatttaccg
    11941 ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg gaagccgccc
    12001 agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat cagtttgcta
    12061 tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg gaagtgttac
    12121 tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc gatcgcattg
    12181 agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc tgtcgattgc
    12241 ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc
    12301 aactgatgca cccaaatatg gtggcggata tgctggaact gttaacccgc tatcgcattc
    12361 agccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac cctcatgctg
    12421 cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg
    12481 gcatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca atcgacgtac
    12541 tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg attgctgcaa
    12601 ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg gagactgaag
    12661 cacaacgcga ctggctggca aaagcgggcg ttggtattgc ccagggcttc ctttttgctc
    12721 acccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag ctaccccaaa
    12781 ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact ttttaacctt
    12841 tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt attttaaggc
    12901 cgcaggtacc ccataacctt acaagacctg tggttttact aaaggacacc ctatgaaaac
    12961 ctctctgttt aaaagccttt actttcaggt cctgacagcg atagccattg gtattctcct
    13021 tggccatttc tatcctgaaa taggcgagca aatgaaaccg cttggcgacg gcttcgttaa
    13081 gctcattaag atgatcatcg ctcctgtcat cttttgtacc gtcgtaacgg gcattgcggg
    13141 catggaaagc atgaaggcgg tcggtcgtac cggcgcagtc gcactgcttt actttgaaat
    13201 tgtcagtacc atcgcgctga ttattggtct tatcatcgtt aacgtcgtgc agcctggtgc
    13261 cggaatgaac gtcgatccgg caacgcttga tgcgaaagcg gtagcggttt acgccgatca
    13321 ggcgaaagac cagggcattg tcgccttcat tatggatgtc atcccggcga gcgtcattgg
    13381 cgcatttgcc agcggtaaca ttctgcaggt gctgctgttt gccgtactgt ttggttttgc
    13441 gctccaccgt ctgggcagca aaggccaact gatttttaac gtcatcgaaa gtttctcgca
    13501 ggtcatcttc ggcatcatca atatgatcat gcgtctggca cctattggtg cgttcggggc
    13561 aatggcgttt accatcggta aatacggcgt cggcacactg gtgcaactgg ggcagctgat
    13621 tatctgtttc tatattacct gtatcctgtt tgtggtgcta gtattgggtt caatcgctaa
    13681 agcgactggt ttcagtatct tcaaatttat ccgctacatc cgtgaagaac tgctgattgt
    13741 actggggact tcatcttccg agtcggcgct gccgcgtatg ctcgacaaga tggagaaact
    13801 cggctgccgc aaatcggtgg tggggctggt catcccgaca ggctactcgt ttaaccttga
    13861 tggcacatcg atatacctga caatggcggc ggtgtttatc gcccaggcca ctaacagtca
    13921 gatggatatc gtccaccaaa tcacgctgtt aatcgtgttg ctgctttctt ctaaaggggc
    13981 ggcaggggta acgggtagtg gctttatcgt gctggcggcg acgctctctg cggtgggcca
    14041 tttgccggta gcgggtctgg cgctgatcct cggtatcgac cgctttatgt cagaagctcg
    14101 tgcgctgact aacctggtcg gtaacggcgt agcgaccatt gtcgttgcta agtgggtgaa
    14161 agaactggac cacaaaaaac tggacgatgt gctgaataat cgtgcgccgg atggcaaaac
    14221 gcacgaatta tcctcttaat ctcaccactt atgcccgcac tcccttcccc gagtgcgggt
    14281 gtttgcgcat aattgacccc tgtgcctgaa cttaccacct gggtgagttc aggtttattt
    14341 cacttcttcc gtgacatttt catgttcttg cggtctaaca cgaagtgttt ttaacgtcat
    14401 ttagactgac tgacaacggg cagtttttta ttaccaggat agttgatcag gggttcacat
    14461 gcagggcaca aaaattcgac ttttagcggg cggtttgctg atgatggcca ctgctggcta
    14521 tgtgcaggca gatgcgctcc agcctgatcc agcatggcaa caggggacgc tttccaacgg
    14581 tttacagtgg caagtgctga ccacccccca gcgtcccagc gatcgtgttg aaattcgcct
    14641 gctggttaat atcggttcgc tcgccgaaag tacacaacag agcggttaca gtcacgccat
    14701 ccctcgtatt gcgctaacgc aaagcggtgg ccttgacgca gcacaggcgc gttcattgtg
    14761 gcagcagggg atcgacccta aacgcccgat gccgccggta attgtctctt atgacaccac
    14821 gctgtttaat ctgagtttgc ccaataaccg taacgatttg ctgaaagaag cgctctctta
    14881 tctggcaaat gccactggca aattgaccat cacaccagaa accatcaacc acgcgctgca
    14941 aagtcaggac atggtggcaa cctggcctgc cgatactaaa gagggctggt ggcgctatcg
    15001 tctgaaaggg tcaaccttgt taggtcacga tcctgccgat ccgctgaaac aacccgttga
    15061 agcggaaaaa attaaagatt tctatcagaa atggtacacc ccggatgcaa tgacgctact
    15121 ggtggtggga aacgtggatg cgcgctcggt tgtcgaccaa atcaacaaaa cgtttggcga
    15181 actgaaaggc aaacgtgaaa cgccagctcc ggtgccgacg ctttctccgc tgcgtgcgga
    15241 agcggtgagt attatgactg acgcggtgcg tcaggaccgg ttatctatca tgtgggatac
    15301 gccgtggcag ccgattcgtg aatcagccgc actgctgcgc tactggcgtg cggacctggc
    15361 ccgcgaggcg ctgttctggc atgttcagca agcgttaagc gccagtaaca gcaaagacat
    15421 cggtcttgga tttgactgcc gtgtgctgta tctgcgtgcg cagtgtgcca tcaacatcga
    15481 atcaccaaac gacaagctga acagcaacct taatctggtg gcgcgtgaac tggcgaaggt
    15541 tcgcgataaa ggtctgccgg aagaagagtt caatgcgtta gtggcgcaaa agaaactgga
    15601 gctgcagaaa ctgtttgccg cctatgcgcg ggctgatacc gatattctga tgggtcagcg
    15661 gatgcgttcg ctgcaaaatc aggtcgtcga tatcgcgccg gagcagtatc agaaactgcg
    15721 gcaggatttc cttaacagcc tgacggtgga gatgttaaat caggatctgc gtcagcagtt
    15781 gtcgaatgat atggcgttaa tactgctgca gccgaaaggc gagccggaat ttaacatgaa
    15841 agcgttgcag gcggcctggg atcaaatcat ggccccatcg actgctgctg ccgccacctc
    15901 tgtcgccacg gatgacgtac atcctgaagt gacggatatt ccacctgcac agtaatgcct
    15961 gatggtgatg agttagcaca accatcaggt atttatggat gagctggata gaggggtaac
    16021 actttatcca gccttttgca tatgctgcgt ttacgctggc atcgcctcac gcgggataat
    16081 cgcgccgcga tactgaataa cggtacttgc ggtcaggtgc ccgcgtttcg ccgcgttttc
    16141 cgcactgccg cccgtcaaac ataccgccag ataaccggcg ctgaaagagt cgccagctgc
    16201 ggtggtatcg atcacttttt ctttcggcag tttcaccgcc ggaacatcca ctaacccttc
    16261 gccagcaatg gacaccaggc aagaatccgc cccgcgtttc accaccactt ctttcacgcc
    16321 cgcgttatgg gtgcgcgcaa tgacgtcttc caccggctgt tgaccccaca gcgcgtcttc
    16381 atcgtccagc gtcaggaagg cgatatccgt gcattccagc atttgttggt acacctgctg
    16441 tgtctcttct ttgctggccc acaggcgcgg acgatagtta ttgtcgaaaa tcacttttcc
    16501 gccgttggcg cggcattcgc gcagcaggga aagcagcttt tcgcggctgg tcgggcttaa
    16561 gatcgccagg ctaatcccgc tcaggtagag ataatcgaaa ttcgccagct cttcgcaaat
    16621 cgccgcagac tgctcactct ccagccagaa tttggcggcg gcttcgttcc gccagtagta
    16681 gaacgtacgc tcgccggtgc tgtcggtttc aatgtagtaa aggcccggca gacggttttc
    16741 catccgttgg gtcagggaag tatcaacgtt ctcgccgtgc caggcgtcca gcatttgctg
    16801 gctaaaactg tccgttccca gcgccgttac gtaatgaacg gttaatgccg caggatcgac
    16861 ctgacgggcg atatagacgg aagtgttcag ggtatcgccg ccgaaaccgc gcttaacgtc
    16921 cgcgcctttc tcggaaagct caatcatgca ttcgccaatc acggcaatct ttttggacat
    16981 agtcgtgaac ctgatctgta aaaaattagc gttagtttgc gctgtggtgg tttgctggtc
    17041 aaccatatta aaacagtgtt ccattatttt tttgagccag aacgtattcc tgaaagattc
    17101 cgttgtggag tgaggaaatt taccccggcc aggcggtaaa gttctgccct tgcgcgccga
    17161 taatctttgt cgagtccggg cagcatcact tttaaacaca ggacatcttt gatgataagg
    17221 caggttatcc agcgaataag caaccctgaa gcaagcatcg agagcttgca ggaacggcgt
    17281 ttttggttgc agtgtgagcg tgcttacacc tggcagccga tctatcaaac atgcggacgg
    17341 ttaatggccg tggagctatt aacggtggtc acgcatccct tgaacccttc gcaacgcctg
    17401 ccgccggatc gctattttac tgaaatcacc gtcagccatc ggatggaggt tgtgaaagag
    17461 cagattgatt tgctggcgca aaaagccgac ttctttgtag agcacgacct gctggcatcg
    17521 gtcaatattg atggccctac gctcatcgcc ctgcgtcagc aaccaaaaat cctgcgccaa
    17581 attgagcgtc ttccctggct gcgtttcgaa ctggtggaac acatccgcct gccgaaagat
    17641 tcaacctttg cctcgatgtg tgaatttggc ccgctgtggc tggatgattt tggcaccggg
    17701 atggcaaatt tctctgcgct aagtgaagtg cgttatgact acatcaaaat cgcgcgagag
    17761 ctgttcgtga tgctgcgtca gtcgccggaa ggccgcacac tcttttctca gcttttacat
    17821 ctaatgaatc gctattgtcg cggggtgatt gtcgagggcg tagaaacacc ggaagagtgg
    17881 cgtgatgttc agaactcgcc cgcattcgcc gcacaaggct ggtttctttc acgcccggca
    17941 ccgatggaaa cgctgaatac ggcggttctg gcgctataag ctgcctcatt ttccgctggg
    18001 ctggactatc tttaggactg gcacaggaag aatatgagga agcgaacgat gagcaaggca
    18061 ggcaaaataa ccgctgcgat ttcaggggct ttcttgttgt tgattgtcgt ggcgatcatt
    18121 ttgattgcaa cttttgactg gaatcgactc aaaccgacca tcaaccagaa agtctctgcg
    18181 gagttgaatc gtccgttcgc tatccgtggc gatctgggcg tggtgtggga gcggcaaaaa
    18241 caagaaactg gctggcgcag ctgggtgccg tggcctcatg tacatgcaga agacatcatt
    18301 cttggtaatc caccggatat tcccgaagtc acgatggtgc atttgccgcg agtagaggca
    18361 acgctggcac cgctggcgct gctgaccaaa acggtctggc tgccgtggat caagctcgaa
    18421 aagcccgacg cgcgcctgat tcgcctctcc gaaaagaaca ataactggac gtttaatctt
    18481 gccaacgatg acaacaaaga cgcgaatgca aagccgtcgg catggtcgtt tcggctggat
    18541 aatattcttt tcgatcaagg gcggatcgcc attgatgaca aagtaagcaa agcggatctg
    18601 gaaatttttg ttgatccgtt aggcaagccg ctgccgttca gcgaagttac tggatcgaaa
    18661 ggtaaagcgg ataaagaaaa ggtgggcgat tacgtttttg gcctgaaggc gcagggacgt
    18721 tataacggtg aaccgctcac gggtacggga aaaataggcg gtatgctggc gctgcgtggc
    18781 gaagggacgc cgtttccggt acaggctgat ttccgttcag gtaatacccg tgttgctttt
    18841 gatggcgtcg tgaatgaccc aatgaagatg ggcggtgtcg atttacggct taaattttct
    18901 ggcgattcac tgggtgatct ctatgaactg acgggcgttc tgctgcccga taccccgccg
    18961 tttgaaacgg atggtcggct ggtagcgaaa atcgacactg aaaaatcgtc ggtctttgat
    19021 tatcgcggtt ttaatgggcg aattggcgat agcgatatcc acggttctct ggtctacacc
    19081 accggaaagc cacgaccaaa actggaaggt gatgtcgagt cgcggcaatt gcggctggcg
    19141 gacctgggac cgttgattgg cgttgattcc gggaaagggg cagaaaagtc gaaacggtct
    19201 gaacagaaga agggcgaaaa aagcgttcag cctgcgggca aagtgctgcc ttatgaccgc
    19261 ttcgaaaccg ataaatggga cgttatggat gccgatgttc gcttcaaagg gcggcgcatt
    19321 gagcatggca gtagcctgcc gattagcgat ctttctactc atatcatcct caaaaatgct
    19381 gacctgcgcc tgcaaccgct gaaatttggc atggcgggcg gcagcattgc ggcgaatatt
    19441 catctggaag gcgataaaaa gccgatgcag gggcgggcag atattcaggc tcgtcgactg
    19501 aaactgaaag aactgatgcc cgatgtggaa ctgatgcaga agacgctggg ggaaatgaac
    19561 ggtgacgcgg aactacgcgg tagcggtaac tcggtggcgg cgcttttagg caacagtaac
    19621 ggcaacctga aactgttgat gaatgacggg ctggtgagcc gcaacctgat ggagattgtt
    19681 gggctgaatg tcggcaacta cattgtcggt gcgatatttg gtgacgatga ggtgcgggtg
    19741 aactgcgcgg cggcgaatct gaatattgcc aacggcgtgg cgcgcccgca gatttttgct
    19801 ttcgatactg agaacgcgtt gattaatgtt accggcacgg caagttttgc ttcagaacag
    19861 ctggatttga ctattgatcc ggagagtaaa gggattcgga ttatcacact gcgttcgccg
    19921 ctgtatgtgc gtgggacgtt taaaaatccg caggctgggg tgaaagccgg accgctgatt
    19981 gcccgtggtg ctgttgctgc ggcactggca acgctggtaa caccgacggc ggcgttactg
    20041 gcactgatct caccttccga aggggaggct aatcagtgtc ggacgatatt gtcgcagatg
    20101 aagaagtgat gataattgcc ggatgtctga catccggcaa ttaaacagat ttagattttt
    20161 ggcaaagtgt gctttgttca tgccggatgc ggcgtgaacg ccttatccgg cctacaaatc
    20221 cttgtgaatt caatatattg tatggaaatt cagtcctgat aagcgtagcg catcaggctt
    20281 ttcacactta ttacaacgac tgatgtcgcg tctcatgggt cagcagcagg gcgattaacg
    20341 tcaagccagc catcgccgcc agatataacc ccaccgcacc taacccgtag ttagtctgca
    20401 accaggctgc gatatacggt gcaacggaag ccccgagaat cgacgctacg ttgtaagaga
    20461 atgatgctcc ggtgtaacgc acttctgttg ggaacagctc tggtaacagc gcgcccatcg
    20521 gcccgaaggt cagccccatc agacttaacc ccagcagcag gaaggcaaaa accagaatcg
    20581 ggttgccaga accgagcagc gggttaaagg cgaacagcgc gaacaggatg atcagcgtgg
    20641 tgataattac catgctttta cggcgaccaa aggcatcagc cagtaatccg gcgactggca
    20701 ccatcacgcc aaaaccaata actgccatca tcagcatcca caacacttcg ttacgcggca
    20761 ggccaagccc aactggcgcg gcggcggtac tgaaggtcat agagtagacc gtcatgatgt
    20821 aaaacagcgt ataggttgcc agcatgatga acgtacccag tacggttacg cgaacatgtt
    20881 tggtcagcag cgtacccagc gggatcttca cctgtttttt cgctttagcg actttctcaa
    20941 acaccggcga ctcatgcagc gacacgcgaa catacaggcc gataatgacc agtaccgccg
    21001 agaagataaa tggcacacgc cagccccagc tcataaactg ctcgtcggtc agtagccagg
    21061 aaagcagcaa gaaagtgccg ttggcaaaga agaagccaat cggtgcgccc agctgtggga
    21121 aggagccata cagtgcacgt ttgcgcggtg gggcgttttc agtcgccagc agcgccgcgc
    21181 cgccccattc accgcctaag cccagaccct gaccaaatcg agccagcgcc agcagcagcg
    21241 gggcgaaaat accaatcgtg gcatagcccg gcagcagacc aatcaccacg gttgaaatcc
    21301 ccatcgttag caacgaggcg accagcgtcg ctttacgccc aacgcgatcg ccaaaatgac
    21361 caaaaacggc agagccaatg gggcgcgcga cgaaggcgat ggcgaaggtg gcgagcgact
    21421 gtagcgttgc tgccgttgga tcgccctgcg ggaagaagat atgcggaaac acaataacgg
    21481 ccgcagtggc gtaaatgtaa aagtcgaaga actcaatggc cgtgccaatg agagaggcga
    21541 caaggacttt attacgcgag ttgatcggcg tgtattcttg ctcgtggtcg agtgttgtgg
    21601 ctgttgcttg cataatcttt tcttattttt tggctaacga atagccatat tacgcacagc
    21661 aaaagtgata tttcaatgtg taacaaatcg cggaaatggt caaaaaccag gcaaaaaggg
    21721 gataatgttt aggccagtga tttttcttct atgaaatgct tcacataatt ttataaatag
    21781 tggataaaac tggttttagg ttaaataaaa gttaccaact ggatttatat gctggggtta
    21841 tgagttggaa tgaaattgtt tgctcccaaa tagatggctg aagaggctct ggggaagagt
    21901 ttcaagaggt tatgtgcgca ttatagccgg atgcgatgct ggcgcatctt atccggccta
    21961 cggggagcca ggccgaataa aatcgtatgc cggataaggc gtttacgccg catccggcct
    22021 attttaaggc tgcgttttcc ctggcattcg cgggtcgtct ttatattcgg cggtggcaat
    22081 ccacgccgcg caaaacagcg tcaaacgggc gaagaagtag aaaaatgcca tcagccccag
    22141 cacggaacca aatgctgcgc cagaggggga tttcatcagc gatggcaggg tgtaggtcat
    22201 cacgatttta atcacttcaa aaccaatagc agccagaaat gttccgcgaa tcagggcttt
    22261 tttgcgcgga cggtggcgcg gtaaccgcca gaagatccag aagaaaagca gatagttggc
    22321 gaagatggaa atcgccaggc caatcaaccg ccacgtcggt ttcagccact caatgctgtt
    22381 caggtgcagg gcgctaataa tcatttgctg cgccgaaccg gcaaccgagg tgatcgaaag
    22441 cgtcacaatc agcgcaatca acaaaccaat cagcgaaata aaatcacgta gatattttac
    22501 ccagaacttc tcctgatctt gcggcgagcg ttcccagaca tcgcgcgact gggcgcgaat
    22561 cgcttcacgc aggttaccca tccagttgat gccggaataa agcgccacag ccaggccgac
    22621 aagccctaca gtcgtacgct gctgaacggc ggtgttgatg gtgtttttca acgtggcggc
    22681 tagcgtcgga tcgctgatgt tttgcagaat tttgtcgaag atatcctgta gcaacatcgg
    22741 atgggaggcc agcacaaagc cccctgcggc aaacgacacc atcaaaatcg ggatcatcga
    22801 caaaaatgag aaataggtga tagccgcacc aaactggtta cccagccgat cgttaaagcg
    22861 ttctgtcgcg cgaatcaggt gcgcaataac cggttggcgt tggacttttt cggcagtggt
    22921 ggtgacggtt tccagcgcct ggctaacttt gccgcctttc tcgctattat ccaactgctt
    22981 aatcggctcg tgctccagat cctgggtggg acgtttgatc tcgttttcct gcgtcatccg
    23041 ctcttttgtc cttctgttgt ggtcattctt aaaaagtata gtcggtcagt ccaggtactc
    23101 tttcattacg ccagccagcc actgcataaa caggtttaca cgccgggaaa gctcccgacg
    23161 ctgcggataa accagcgaaa gggagagcgg ctcggcacgg tagcctggca acacttcaat
    23221 aagctgcccg gcacgcaggg cttcgcgcac ggcaatgcgc gggatctgaa taatccccag
    23281 accggcaaga ccggcggcga gatagttttc gctactgttt accgtcaaca tgccgccaga
    23341 cttaaaccac tggacgccat taacgctggc aacctcaaaa cctaacggat gtacacccag
    23401 gtgcggtgta taacgcacca ttgcgtgtga agtcagatca tcggggcttt gcggataacc
    23461 aaagcgcgtc agatagtgcg gactggcaca gttgaccatg gtcagtttgc cgaggggacg
    23521 ggcgataacg ccatcttccg gtaacgcgcc agtgcgtatc acgcaatcaa aaccatcatg
    23581 aagaatatct accggacggt tatggctact cagttccagc tcaattcccg gatgctgata
    23641 gagaaattcc gacagacgcg gcagtaacag gtttttcgcg attcctgacg ggatgtcgat
    23701 gcgtaattta ccgctgatac tggtggcatc ctgttgaaac agaccgtcca gttcgctgag
    23761 attactcaac acatcttttg cccgttgata ataggtcatg ccttctggcg tgagtttgac
    23821 ccgtcgcgta gtgcgctgga gaagctgggt gccaagttga tgttccagtg cctgtatctg
    23881 gcgcgaaaca cttccctttg gcaaagcaaa gaaatccgcc gcgcgggaaa aactttccag
    23941 ctccgcgact ttgatgaaca actgcattgc gtgaatttta tccatttctt tgaccgattg
    24001 ttgttcacag cgaaacagta acgcgcatat cgcattgttt attgtttttt ctaacatcta
    24061 atgccgggtt tcacaatgtt gtcatgacga atgtggcgat ttctcagcag gaaaagggcg
    24121 gaatagcttt atgagaaagg tgaatcccct taaacagagc gtatttttca gtgattccct
    24181 ttaaagtgaa tagaggacgt attggctatt tgtgcttatt tttgtctcat tgccatttgt
    24241 tattttggct tgtagtcgcg gaatttcatt tttaattgca tgcgtactaa ccaccaatat
    24301 aaggaaatcc tcaaaattgt tttaaatagt gccctcgaca tgtaatttca ggcgtttagc
    24361 tgtaactatt tatatcggta actattttct acgcgtcatc tctgtagaat ggccaccgcc
    24421 aaatggcgaa ttcttatttt ttctcgcggg taataccctt ccactactta agtcttttta
    24481 acgtattcag gtggagattc agttatgcaa atagtcatgt ttgacaggca gtcaatattt
    24541 attcatggaa tgaaaatcag tttacagcag cgtattccag gagtgagtat tcagggggcc
    24601 agtcaggcag acgagttatg gcaaaagctg gaaagttacc ctgaagcctt agttatgctc
    24661 gatggcgatc aggatggtga gttttgctac tggttgctgc aaaaaaccgt ggtgcagttt
    24721 cctgaggtta aggtgttaat cacggcgacg gattgcaata aacggtggtt acaggaagtt
    24781 attcatttta atgtgctggc cattgtgcct cgtgattcaa ccgtcgagac atttgcgttg
    24841 gcggtaaata gtgcggcgat ggggatgatg tttctgcctg gagactggcg gacgacgccg
    24901 gaaaaggaca ttaaagatct caaatcgtta agcgcccgcc agcgggagat tttaaccatg
    24961 ttagcggcgg gcgaatcaaa taaggagatc ggcagagcgc tgaatatcag taccggaacg
    25021 gtaaaagcgc atctggaatc cctgtatcgc cgtctggaag tgaaaaatcg cacccaggcg
    25081 gcaatgatgt taaatatctc ctcctg
//
LOCUS       DAESHI010000055        24934 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.055, whole genome shotgun sequence.
ACCESSION   DAESHI010000055 DAESHI010000000
VERSION     DAESHI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24934
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.055"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(382..1359)
                     /gene="epmA"
                     /locus_tag="KW011_002330"
     CDS             complement(382..1359)
                     /gene="epmA"
                     /locus_tag="KW011_002330"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HBH7660643.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1684..3492
                     /gene="frdA"
                     /locus_tag="KW011_002331"
     CDS             1684..3492
                     /gene="frdA"
                     /locus_tag="KW011_002331"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBH7660644.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3485..4219
                     /gene="frdB"
                     /locus_tag="KW011_002332"
     CDS             3485..4219
                     /gene="frdB"
                     /locus_tag="KW011_002332"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HBH7660645.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4230..4625
                     /gene="frdC"
                     /locus_tag="KW011_002333"
     CDS             4230..4625
                     /gene="frdC"
                     /locus_tag="KW011_002333"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBH7660646.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4636..4995
                     /gene="frdD"
                     /locus_tag="KW011_002334"
     CDS             4636..4995
                     /gene="frdD"
                     /locus_tag="KW011_002334"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBH7660647.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5058..6191
                     /locus_tag="KW011_002335"
     CDS             5058..6191
                     /locus_tag="KW011_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBH7660648.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6280..6813
                     /gene="blc"
                     /locus_tag="KW011_002336"
     CDS             6280..6813
                     /gene="blc"
                     /locus_tag="KW011_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBH7660649.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="KW011_002337"
     CDS             complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="KW011_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBH7660650.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="KW011_002338"
     CDS             complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="KW011_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBH7660651.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="KW011_002339"
     CDS             complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="KW011_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBH7660652.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7736..8302)
                     /gene="efp"
                     /locus_tag="KW011_002340"
     CDS             complement(7736..8302)
                     /gene="efp"
                     /locus_tag="KW011_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBH7660653.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8344..9372
                     /gene="epmB"
                     /locus_tag="KW011_002341"
     CDS             8344..9372
                     /gene="epmB"
                     /locus_tag="KW011_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBH7660654.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9762..10631
                     /locus_tag="KW011_002342"
     CDS             9762..10631
                     /locus_tag="KW011_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HBH7660655.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLNAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGTVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(10834..11187)
                     /locus_tag="KW011_002343"
     CDS             complement(10834..11187)
                     /locus_tag="KW011_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HBH7660656.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(11325..12971)
                     /gene="groL"
                     /locus_tag="KW011_002344"
     CDS             complement(11325..12971)
                     /gene="groL"
                     /locus_tag="KW011_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBH7660657.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(13015..13308)
                     /locus_tag="KW011_002345"
     CDS             complement(13015..13308)
                     /locus_tag="KW011_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBH7660658.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            13584..14840
                     /gene="yjeH"
                     /locus_tag="KW011_002346"
     CDS             13584..14840
                     /gene="yjeH"
                     /locus_tag="KW011_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HBH7660659.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(14862..15332)
                     /gene="fxsA"
                     /locus_tag="KW011_002347"
     CDS             complement(14862..15332)
                     /gene="fxsA"
                     /locus_tag="KW011_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HBH7660660.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            15669..17105
                     /gene="aspA"
                     /locus_tag="KW011_002348"
     CDS             15669..17105
                     /gene="aspA"
                     /locus_tag="KW011_002348"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HBH7660661.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            17223..18524
                     /gene="dcuA"
                     /locus_tag="KW011_002349"
     CDS             17223..18524
                     /gene="dcuA"
                     /locus_tag="KW011_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HBH7660662.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            18640..18978
                     /gene="cutA"
                     /locus_tag="KW011_002350"
     CDS             18640..18978
                     /gene="cutA"
                     /locus_tag="KW011_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HBH7660663.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            18954..20651
                     /gene="dsbD"
                     /locus_tag="KW011_002351"
     CDS             18954..20651
                     /gene="dsbD"
                     /locus_tag="KW011_002351"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HBH7660664.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            20688..21263
                     /locus_tag="KW011_002352"
     CDS             20688..21263
                     /locus_tag="KW011_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH7660665.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            21370..21445
                     /locus_tag="KW011_002353"
     tRNA            21370..21445
                     /locus_tag="KW011_002353"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21403..21405,aa:Phe,seq:gaa)
     gene            22062..23599
                     /gene="cadC"
                     /locus_tag="KW011_002354"
                     /pseudo
     CDS             22062..23599
                     /gene="cadC"
                     /locus_tag="KW011_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
     gene            23964..>24934
                     /gene="cadB"
                     /locus_tag="KW011_002355"
     CDS             23964..>24934
                     /gene="cadB"
                     /locus_tag="KW011_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HBH7660666.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLL"
BASE COUNT         6222 a         6267 c         6122 g         6323 t
ORIGIN      
        1 aggcgaatgg aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac
       61 ccattaagta ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca
      121 atatgagtcc tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt
      181 cagcgcgtgt ataacacgtg cccactgaac tgcactgcgc aacattgagc aggcagcgta
      241 aaaaccaata cagaattgat acccggatga tatcaaatat ccagtcttaa attgtggcct
      301 ttaacggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgctgt
      361 taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag
      421 tgtctccgcg cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca
      481 gtcaggcata ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa
      541 accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc
      601 cgttaattca tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc
      661 agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg
      721 gtacacaaag gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa
      781 ttgtagcaac gtgtcgcggt cttcttcggt atcagcaacg ttactcaaat ccagtttcgc
      841 tgcgacttcc cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa
      901 gaaagcttgt tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag
      961 atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc actcaagcat
     1021 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg
     1081 gaataccggc ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat
     1141 taaccagaga ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac
     1201 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
     1261 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcacgtttta ataagttagg
     1321 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
     1381 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac
     1441 agcggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
     1501 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
     1561 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
     1621 taactttcag gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat
     1681 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
     1741 gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta
     1801 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
     1861 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
     1921 caggatgtcg tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg
     1981 tgggggtgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
     2041 atgaaaatcg agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg
     2101 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg
     2161 gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc
     2221 acgctggtgc agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt
     2281 tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
     2341 cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt
     2401 tccggtatcc tgatgaccga aggctgccgt ggtgaaggcg gtattctggt caacaaaaat
     2461 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
     2521 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
     2581 cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cttgcgtcac
     2641 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
     2701 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
     2761 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
     2821 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa
     2881 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
     2941 aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat
     3001 ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca
     3061 atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
     3121 ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc
     3181 aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg
     3241 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
     3301 tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat
     3361 ggcacgactc gcctagagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc
     3421 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
     3481 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
     3541 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
     3601 tgcgctgggc tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg
     3661 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
     3721 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
     3781 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
     3841 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
     3901 gatggcaaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
     3961 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
     4021 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
     4081 gaacggcgta tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt
     4141 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
     4201 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
     4261 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag
     4321 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
     4381 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga
     4441 tcattaacct gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg
     4501 caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
     4561 aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
     4621 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
     4681 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
     4741 gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt
     4801 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
     4861 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct
     4921 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
     4981 atcgttacaa tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg
     5041 tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt
     5101 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
     5161 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
     5221 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
     5281 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
     5341 ctcgagggga aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttactgcta
     5401 aacagtggaa tgggatcaca ctattacatc ttgcgaccta caccgctggc ggcctgccat
     5461 tgcaggtgcc ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc
     5521 agcctgcatg ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
     5581 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct
     5641 tccagccact caaactcaac catacgtgga ttaatgtacc tcccccagaa gaaaagaatt
     5701 acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg
     5761 aagcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttaa
     5821 ccccccttga tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct
     5881 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca
     5941 atcctgacag catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa
     6001 aagcgattaa gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga
     6061 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc
     6121 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg
     6181 ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct
     6241 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
     6301 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc
     6361 gtgacagtag tgaataattt cgacgctaaa cgctatctcg gtacctggta tgagattgcc
     6421 cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata cagtctgcgt
     6481 gatgacggcg gcctgaatgt catcaataaa ggctataacc ctgacagaga aatgtggcag
     6541 cagagtgaag ggaaagcgta ctttaccggc gcaccaacac gcgctgcgct gaaagtgtca
     6601 ttctttggtc ctttctatgg cggttataac gtcattgcac ttgatcggga ataccgccat
     6661 gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
     6721 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
     6781 aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc
     6841 cccaatacga ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc
     6901 acaatgccgg tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc
     6961 cctaccggta acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc
     7021 acagtaataa cacttggcgt caaacgacta aagccgtggg tatatttcag gccaacggcc
     7081 cataccactt ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga
     7141 gaattggggc cgtccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt
     7201 tgaacggata ttttgccagg tggcagggga atttcaacta ttacctttgt gactatcgca
     7261 aaaaaacaaa cggcacgaca taactgtcgt accgttattg cttattgctg cgctttcgtt
     7321 gctgcaccag aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg
     7381 ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc
     7441 ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata
     7501 atagacaaca tcgccaaaaa tgatggaaat ctgatgattt ttcggaagag aagaaaaatt
     7561 agctggcagc gcgggagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag
     7621 cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca
     7681 tcatctctga cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact
     7741 tcacgcgaga gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta
     7801 cgaacagcgg aactttaacc acagcgccag tagacagggt agccggtttg ccgccagtac
     7861 ctgcggtatc acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg
     7921 gcggagtaac ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca
     7981 gcagccattt agcgttgtcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt
     8041 cgttgttcat gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga
     8101 caacatcagc gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg
     8161 tcagcagacg acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt
     8221 cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat
     8281 cgttgctata gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac
     8341 aaaatggcgc atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt
     8401 gccgatgttg tgaccgatcc tggtgaactt ctgcgtcttt tgaatataga cgcggacgaa
     8461 aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt
     8521 atcgatcgca tggagaaagg caatccggac gatcctattt tgcgtcaggt acttacctcg
     8581 caagatgagt ttgtcgtcgc gtccggattc tccaccgacc cactggaaga acagcacagc
     8641 gtagtgcctg gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc
     8701 tgcgcggtaa attgccgcta ttgcttccgt cgccacttcc cctatgccga aaatcagggc
     8761 aacaagcgta actggcagac tgcacttgag tatgttgctg cgcatccgga actggacgag
     8821 atgattttct ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc
     8881 acacaactgg aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt
     8941 gtgatcccgg cacgtatcac cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa
     9001 atcttgctgg tgaatcacat caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg
     9061 atggctaaat tgcgccgtgt cggtgtcacc ctgcttaacc agagcgttct gttacgtggt
     9121 gtgaacgata acgcacaaac gttggcaaac ctgagtaatg cgttgttcga tgccggcgtg
     9181 atgccgtatt acctgcatgt gctagataaa gtacagggcg cggcgcattt tatggtgagt
     9241 gatgacgaag cacggcagat tatgcgtgag ttgctgacat tggtgtcggg atatctggtg
     9301 ccgaaactgg cgcgagaaat tggcggcgaa cccagcaaaa cgccgctgga tctccagcta
     9361 cgccagcagt aactgattaa acagggaagt taaacattag cctgatgcgc tacgcttatc
     9421 aggcctacat tgtgcattga aattgaatgg atttgcaatg aactgtaggc cggataaggc
     9481 gtttacgccg catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc attaaactgg
     9541 gaaatttaat ctttgcccgt tgcataacct ttaattattc atatacattg ctgttgttac
     9601 tctttatata gccaacacca ataattaacc tttcttttct gtttatatag agtgagtacg
     9661 gctatttatg ttattttaac aactattact accaagccca tttatctggc ttataaatta
     9721 aaattaatta tttaattgag aaatggttag ggagaaccta catggccata agcatcaagg
     9781 gtgttaatac cggtgttatt cgcaagagta ataatttcat tgcactggcg ctgaaaatta
     9841 aagaacctcg caataaagaa tcgttgttct ttatgtctgt catggaactg cgcgacctgc
     9901 tgattgcgct ggaaagtcgt ctgcatcaaa agcataaact gaatgctgct gcacgccttc
     9961 agtatgagca agctcgcgat aaagtcatta agaaaatggc agaaaacatc ccagaaattc
    10021 tggttgatga gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg
    10081 ataatcaagg cgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt
    10141 tagtcgtcaa tgagttgcag attgaaatgc tggcgcgagc aatcattcat gcaattaata
    10201 atgcggaaat gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat
    10261 acgacgttga ctgccaggac aatggcaatc tggaatatga cacctattcc caaccggaat
    10321 ggaaacataa cctgttcgac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg
    10381 gcaaagaaca atttagcggc acagtggtga aaacgcgtga agcaacgccg ggtaaagaaa
    10441 tagaggcgat cactcgccga atgcttgatt tcagcccacg actgaaaaaa ctcgcgggtg
    10501 tgccatgtca ggtctatgtt cgcaccgtag cggcgaataa cgcccaaccg ctaacccagg
    10561 atcaatgctt acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac
    10621 aggcgaaata atctcatctt tgctaatgcg aggctggagc catccagcct gaaatgactg
    10681 ataacgtgat cgttggtcat gccggatgcg acgtaaacgc cttatccggc ctacaaattc
    10741 tcaagcattc aatcagttgc aatgcctgct gtaggcctga taagcgaagc gcatcaggca
    10801 ataatacgtt tgtcacccag cctctggatc acatcagttc gggcacttat aaacctgacc
    10861 gataatctgg ctatccgtcg ggacaaaact ggacaacatt ccctgcgacg ggctgctgat
    10921 gccataaatc acgttaccgc ccattgcagc cgcctggttg cgcagatcgt ttgctgcgcc
    10981 gcgcatagaa ccgccctctt ctccgtgttg cccggaaagc cagttgcttt gcttacctgt
    11041 cgcagtacca atcagctggc actctgcgcc tggctgctcg tccacaatgc gtacactctg
    11101 accggcagca ctcaattcgt tgctggagct acaacccgcc atcagtagcg cggcaccgac
    11161 aatccctgct aagtatttta cgtgcatgtt attccccata atcaatgagc tggacgccac
    11221 tcgcgtcgtc cgtgtctgaa tcttatacta gaaagatgac caaaagaaaa acccccagac
    11281 atttctgccc gggggtttgt ttatttctgc gaggtgcagg gcaattacat catgccgccc
    11341 atgccaccca tgccgcccat accgccagca gcgcctaagt cagctgcatc atttttcggc
    11401 aggtcggtaa ccatgcactc ggtggtgatc atcaggccag ccacagaagc cgcgtactgc
    11461 agagcagaac gggttacttt ggttgggtcc aggataccca tgtcgatcat gttgccgtat
    11521 tcttcggttg ctgcgttgta accgtagttg ccgtcgccgc ctttaacggt gttagcaaca
    11581 acagacggtt cttcgccgca gttcaggacg atctgacgca gcggagcttc cattgcacgc
    11641 agtgcaactt tgatacccac gttctggtct tcgttctgac cacgcaggtc agccagttta
    11701 gacgctacgc ggatcagcgc aacaccacca ccagcaacca cgccttcttc taccgcagca
    11761 cgggtcgcgt gcagggcgtc ttcaacgcgt gcttttttct ctttcatttc aacttcggta
    11821 gcagcaccta ctttgataac tgcaacgccg cctgccagtt tcgctacgcg ttcctgcagt
    11881 ttttcacggt cgtagtcaga agttgcttct tcaatctgct gacggatctg agcaacacgg
    11941 ccctggattg cagcttcttc acccacgcca tcgatgatag tggtggtgtc tttgttgatc
    12001 acaacacgtt tagcctgacc caggtcttcc agggttgctt tttccagttc cataccgatc
    12061 tcttcagaga tcacggtacc gccagtcagg gttgcgatat cctgcagcat agctttacga
    12121 cgatcgccga agcccggagc tttaactgca gcaactttca cgatgccacg catggtgtta
    12181 acaaccagag ttgccagcgc ttcgccttct acatcttcag cgatgatcag cagcggtttg
    12241 cctgctttgg caacggcttc cagaaccggc agcatttcgc ggatgttgga gattttcttg
    12301 tcagccagca ggatgaacgg gctttccagt tctactgcgc cagtttccgg cttgttgatg
    12361 aagtaaggag acaggtagcc acggtcgaac tgcatacctt caaccacgtc cagttcgtcc
    12421 tgcagaccgg taccgtcttc aacggtgata acgccttctt taccgacttt gtccatcgct
    12481 tcagcgatca gtttacctac ggtttcgtcg gagttagcgg agatggtacc aacctgagca
    12541 atcgctttag agtcagagca cggtacggac agcgctttca gttcttcaac tgcagcggta
    12601 acggctttgt cgataccacg tttcaggtcc atcgggttca tgcccgcagc aacagctttc
    12661 agaccctcag tgatgatagc ctgagccagt acagttgcag tggtggtacc gtcgcctgca
    12721 gcgtcgttcg ctttagaggc aacttctttc accatctgcg cacccatgtt ttcgaacttg
    12781 tcttccagtt cgatttcacg agcaacggaa acaccatctt tggtgatggt cggtgcaccg
    12841 aaagatttat ccagaactac gttacggcct ttcggaccga gggtaacttt cactgcatct
    12901 gccagtacgt ttacgccgcg cagcattttc acacgagcgt cgttaccgaa ttttacgtct
    12961 ttagctgcca ttatctttat tccttaaatt cgtatgttca gtgtcgtgcg aggattacgc
    13021 ttcaacaatt gccagaatgt cgctttcgga catgatcaac acttcttcat tgtcgatctt
    13081 ctcagatttc acaccgtagc catcgttgaa aataacgatg tcgccaactt tcacatccag
    13141 cggcttcact tcgccatttt caaggatacg gccattgccg acagccagca cttcgccgcg
    13201 ggtggattta gccgctgcag agccggtcag aacgatgccg ccagcagatt tagtttcaac
    13261 ttctttacgc ttgacgatca cgcgatcatg caatggacga atattcattg ataactctcc
    13321 tttgagaaag tccgtatctg ttatgggtga cgccggagct tacgtggttt cccggctggt
    13381 gaccagagaa atggggatga ggattcgccc cttcaagggg gaaaaagaaa aaaaattctg
    13441 atcagcacaa aatcgggtga aaaccctgat tcacctcaca tttcatcgca atttcttcat
    13501 cgccgtcata agcgaatctg attgtgctac catcgaaaat ctacgcagtt gcccaaaatt
    13561 tgggcgcaat cggatatcaa cccatgagtg gactcaaaca agaactgggg ctggcccagg
    13621 gcattggcct gctatcgacg tcattattag gcaccggcgt gtttgccgtt cctgcgttag
    13681 ctgcacaggt agcaggcaat aacagcctgt gggcgtggcc cgttttgatt atcttagtgt
    13741 tcccgattgc gattgtgttt gcgattctgg gtcgccacta tcccagcgca ggcggtgtcg
    13801 cgcacttcgt cggtatggcg tttggttcgc ggcttgagcg agtcaccggc tggttgtttt
    13861 tatcggtcat tcccgtgggt ttgcctgccg cgctacaaat tgccgccggg ttcggccagg
    13921 caatgtttgg ctggcatagc tggcaactgt tgttggcaga actcggtacg ctggcgctgg
    13981 tgtggtatat cggtactcgc ggtgccagtt ccagtgctaa tctacaaacc gttattgccg
    14041 gacttatcgt cgcgctgatt gtcgctatct ggtgggcggg cgatatcaaa cctgcgaata
    14101 tccccttccc ggcacctggt aatatcgaac ttaccgggtt atttgctgcg ttatcagtga
    14161 tgttctggtg ttttgtcggt ctggaagcat ttgcccatct tgcctcggaa tttaaaaatc
    14221 cagagcgtga ttttcctcgc gcattgatga ttggtctgct gctggcagga ttagtctact
    14281 ggggctgtac ggtagtcgtc ttacacttcg acgcctatgg tgaacaaatg gccgcggcag
    14341 catcgcttcc aaaaattgta gtgcaactgt tcggtgtagg agcgttatgg attgcctgcg
    14401 taattggcta tctgacctgc tttgccagtc tcaacattta tatacagagc ttcgcccgcc
    14461 tggtctggtc gcaggcgcaa cataatcctg accactacct ggcacgcctc tcttctcgcc
    14521 atatcccgaa taatgccctc aatgcggtgc tcggctgctg cgtggtgagc actttggtga
    14581 ttcatgcttt agagatcaat ctggacgctc ttattattta tgccaatggc atctttatta
    14641 tgatttatct gttatgcatg ctggcaggct gtaaattatt gcaaggacgt tatcgactac
    14701 tggcggtggt tggcgggctg ttatgcgttc tgttactggc aatggtcggc tggaaaagtc
    14761 tctatgcgct gattatgctg gcggggttat ggctgtttct gccaaaacga aaaacgccgg
    14821 aaaatggcat aaccacataa tccggcgttt cgacattaat cctagcgatc gtctttatga
    14881 tcaaggcggt cgcggtcatc atcctttcgc tggtactcac catcaaaagt attaccgcca
    14941 ccggtcccgg cgctaaaacc gccgcctggc atgcgagaaa agcgcaaatg tggcatcaac
    15001 ttcactgtca gatgcttttg caccggcggc aataaaagca gaagaccgag gaagtcggtg
    15061 aaaaagcccg gtaataaaag cagcaaaccg gcaataatca gcgaaacact tttaatcatc
    15121 tccgccgcag ggttttcacc cgccgccatt ttttgctgca tcagcacgaa attcttaaag
    15181 ccctggttac gtaccagtga cataccgata actgacgtga atataaccag cacgagggtc
    15241 agcaataccc ccaatacatg ggcaacctga ataaagattg aaatctcaat atagacataa
    15301 aggaaaatgg caataaaagg taaccagcgc aaaggtttct cctgtaatag cagccggtta
    15361 aacccggcta cctgaatggg ttgcgaatcg cgttcagctt atattgtggt cattagcaaa
    15421 atttcaagat gtttgcgcaa ctatttttgg tagtaatccc aaagcggtga tctatttcac
    15481 aaattaataa ttaaggggta aaaaccgaca cttaaagtga tccagattac ggtagaaatc
    15541 ctcaagcagc atatgatctc gggtattcgg tcgatgcagg ggataatcgt cggtcgaaaa
    15601 acattcgaaa ccacatatat tctgtgtgtt taaagcaaat cattggcagc ttgaaaaaga
    15661 aggttcacat gtcaaacaac attcgtatcg aagaagatct gttgggtacc agggaagttc
    15721 cagctgatgc ctactatggt gttcacactc tgagagcgat tgaaaacttc tatatcagca
    15781 acaacaaaat cagtgatatt cctgaatttg ttcgcggtat ggtaatggtt aaaaaagccg
    15841 cagctatggc aaacaaagag ctgcaaacca ttcctaaaag tgtagcgaat gccatcattg
    15901 ccgcatgtga tgaagtcctg aacaacggaa aatgcatgga tcagttcccg gtagacgtct
    15961 accagggcgg cgcaggtact tccgtaaaca tgaacaccaa cgaagtgctg gccaatatcg
    16021 gtctggaact gatgggtcac cagaaaggtg aatatcagta cctgaacccg aacgaccatg
    16081 ttaacaaatg tcagtccact aacgacgcct acccgaccgg tttccgtatc gcagtttact
    16141 cttccctgat taagctggta gatgcgatta accaactgcg tgaaggcttt gaacgtaaag
    16201 ctgtcgaatt ccaggacatc ctgaaaatgg gtcgtaccca gctgcaggac gcagtaccga
    16261 tgaccctcgg tcaggaattc cgcgctttca gcatcctgct gaaagaagaa gtgaaaaaca
    16321 tccaacgtac cgctgaactg ctgctggaag ttaaccttgg cgcaacagca atcggtactg
    16381 gtctgaacac gccgaaagag tactctccgc tggcagtgaa aaaactggct gaagtcactg
    16441 gcttcccatg cgtaccggct gaagacctga tcgaagcgac ctctgactgc ggcgcttatg
    16501 ttatggttca cggcgcgctg aaacgcctgg ctgtgaagat gtccaaaatc tgtaacgacc
    16561 tgcgcttgct ctcttctggt ccacgtgccg gcctgaacga gatcaacctg ccggaactgc
    16621 aggcgggctc ttccatcatg ccagctaaag taaacccggt tgttccggaa gtggttaacc
    16681 aggtatgctt caaagtcatc ggtaacgaca ccactgttac catggcagca gaagcaggtc
    16741 agctgcagtt gaacgttatg gagccggtca ttggccaggc tatgttcgaa tccgttcaca
    16801 ttctgaccaa cgcttgctac aacctgctgg aaaaatgcat taacggcatc actgctaaca
    16861 aagaagtgtg cgaaggttac gtttacaact ctatcggtat cgttacttac ctgaacccgt
    16921 tcatcggtca ccacaacggt gacatcgtgg gtaaaatctg tgccgaaacc ggtaagagtg
    16981 tacgtgaagt cgttctggaa cgcggtctgt tgactgaagc ggaacttgac gatattttct
    17041 ccgtacagaa tctgatgcac ccggcttaca aagcaaaacg ctatactgat gaaagcgaac
    17101 agtaatcgta cagggtagta caaataaaga aggcacgtca gatgacgtgc cttttttctt
    17161 gtgagcagta acttaaaaat aacaatctaa tatcaacttg ttaaaaaaca aggaaggcta
    17221 atatgctagt tgtagaactc atcatagttt tgctggcgat cttcttgggc gccagattgg
    17281 ggggaatagg tattggtttt gcaggcggat tgggggtgct ggttcttgcc gctattggcg
    17341 ttaaacccgg taacatcccg ttcgatgtca tttccattat catggcggtt atcgccgcta
    17401 tttctgccat gcaggttgct ggcggtctgg actatctggt tcatcagaca gaaaagctgc
    17461 tgcgccgtaa cccgaaatac atcacaatcc tcgcaccgat cgtgacctat ttcctgacaa
    17521 tctttgctgg tactggcaac atctctctgg cgacactgcc agttatcgct gaagttgcga
    17581 aggaacaagg cgttaaacct tgccgtccgc tgtctactgc agtggtatcc gcgcagattg
    17641 cgatcaccgc atcgccaatc tcagcggcag tggtttacat gtcttccgtg atggaaggtc
    17701 atggcatcag ctacctccat ctgctctccg tggtcatccc gtccaccctg ctggcggttc
    17761 tggtgatgtc cttcctggtc actatgctgt tcaactccaa actctctgac gatccgattt
    17821 atcgcaagcg tctggaagag ggcctggttg aactgcgcgg tgaaaagcag attgaaatca
    17881 aatccggtgc aaaaacgtcc gtctggctgt tcctgctggg cgtagttggc gtggttatct
    17941 atgcaatcat caacagccca agcatgggtc tggttgaaaa accgctgatg aacaccacca
    18001 acgcaatcct gatcatcatg ctcagcgttg caactctgac caccgttatc tgtaaagtcg
    18061 ataccgacaa catcctcaac tccagcacct tcaaagcagg tatgagcgcc tgtatttgta
    18121 tcctgggtgt tgcgtggctg ggcgatactt tcgtttccaa caacatcgac tggatcaaag
    18181 ataccgctgg tgaagtgatt cagggtcatc cgtggctgct ggccgtcatc ttcttctttg
    18241 cttctgctct gctgtactct caggctgcaa ccgcaaaagc actgatgccg atggctctgg
    18301 cactgaacgt ttctccgctg accgctgttg cttctttcgc tgcggtgtct ggtctgttca
    18361 ttctgccgac ctatccgacg ctggttgctg cggtacagat ggatgacacg ggtacaaccc
    18421 gtatcggtaa attcgtcttc aaccatccgt tcttcatccc gggtactctg ggtgttgccc
    18481 tggccgtttg cttcggcttc gtgctgggta gcttcatgct gtaatgacca atcgcggggc
    18541 gttcacgccc cgctttcttt cccgccgact aacatccttc ccccgtccgt tgtatagtga
    18601 cctctctctt gcggttccat ctgttcttgc gaggtgttta tgcttgatga aaaaagttcg
    18661 aataccgcgt ctgtcgtggt gctatgtacc gcaccggatg aagcgacagc ccaggattta
    18721 gccgccaaag tgctggcgga aaaactggcg gcctgcgcga ccttgatccc cggcgctacc
    18781 tctctctatt actgggaagg taagctggag caagaatacg aagtgcagat gattttaaaa
    18841 actaccgtat ctcaccagca ggcactgctg gaatgcctga agtctcatca tccatatcaa
    18901 accccggaac ttctggtttt acctgttaca cacggagaca cagattacct ctcatggctc
    18961 aacgcatctt tacgctgatc ctgctacttt gcagcacttc cgtttttgcc ggattattcg
    19021 acgcgccggg acgttcacat tttgtccccg cggatcaagc ctttgctttt gattttcagc
    19081 aaaaccaaca tgacctgaat ctgacctggc agatcaaaga cggttactac ctctaccgta
    19141 aacagatccg cattacgccg gaacacgcga aaattgccga cgtgcagctg ccgcaaggcg
    19201 tctggcatga agatgagttt tacggcaaaa gcgagattta ccgcgatcgg ctgacgcttc
    19261 ccgtaaccat caaccaggcg agtgcgggag cgacgttaac tgtcacctac cagggctgtg
    19321 ctgatgccgg tttctgttat ccgccagaaa ccaaaaccgt tccgttaagc gaagtggtcg
    19381 ccaacaacgc agcgtcacag cctgtgtctg ttccgcagca agagcagcac accgcgcaat
    19441 tgcccttttc cgcgctctgg gcgttgttga tcggtattgg tatcgccttt acgccatgcg
    19501 tgctgccaat gtacccactg atttctggca tcgtgctggg tggtaaacag cggctctcca
    19561 ctgccagagc attgttgctg acctttattt atgtgcaggg gatggcactg acttacacgg
    19621 cgctgggtct ggtagttgcc gccgcagggt tacagttcca ggcggcgcta cagcacccat
    19681 acgtgctcat tggcctcgcc atcgtcttta ccttgctggc gatgtcaatg tttggcttgt
    19741 ttactctaca actcccctct tcgctgcaaa cacatctcac attgatgagc aatcgccaac
    19801 agggcggctc acctggcggt gtgtttgtta tgggggcgat tgccggactg atctgttcac
    19861 catgcaccac cgcaccgctt agcgcgattc tgctgtatat cgcccaaagc gggaacatgt
    19921 ggctgggcgg cggcatgctt tatctctatg cgttgggcat gggcctgccg ctgatgctaa
    19981 ttaccgtctt tggtaaccgc ttgctgccga aaagcggccc gtggatggaa caagtcaaaa
    20041 ccgcgtttgg ttttgtgatc ctcgcactgc cgatcttcct gctggagcga gtgattggtg
    20101 atatatgggg attacgcttg tggtcggcgc tgggtgtcgc attctttggc tgggccttta
    20161 tcaccagcct acaggccaaa cgcggctgga tgcgcgtggt gcaaataatc ctgctggcag
    20221 cggcattggt tagcgtgcgc ccacttcagg attgggcatt tggtgcgacg cataccgcgc
    20281 aaactcagac gcatctcaac tttacacaaa tcaaaacggt agatgagtta aatcaggcgc
    20341 tcgttgaagc caaaggcaaa ccggtgatgt tagatcttta tgccgactgg tgcgtcgcct
    20401 gtaaaaagtt tgagaaatac accttcagcg acccgcaggt gcaaaaagcg ttagcagaca
    20461 cggtcttact tcaggccaac gtcactgcta acgacgcaca agatgtggcg ctgttaaagc
    20521 atcttaatgt ccttggacta ccgacaattc tcttttttga cggacaaggc caggagcatc
    20581 cacaagcacg cgtcacgggc tttatggatg ctgaaacctt cagcgcacat ttgcgcgatc
    20641 gccaaccgtg aacgacactt gcagtgggaa agacggagga aaatagcgtg caacgtgaag
    20701 atgtactggg agaagccctg aaattattag aattacaagg gattgccaac accacgctgg
    20761 agatggttgc tgaacgtgtg gattatccac tggacgagct acgccgcttc tggccagaca
    20821 aagaggcaat cctctacgat gcgctgcgtt atcttagcca acagatagat gtctggcgtc
    20881 gtcagctgat gcttgacgaa acgcaaaccg ccgaacaaaa gctgctggca cgttatcagg
    20941 cgttatcgga gtgcgttaaa aacaaccgct atccgggctg tctatttatc gctgcctgta
    21001 cgttttatcc cgaccctggc caccctattc atcaactggc cgatcagcaa aaaagcgcgg
    21061 cctacgattt cacccacgaa ctgttaacca cgctggaagt tgacgatccg gcgatggtag
    21121 caaagcagat ggaactggtg ctggaaggct gtttaagccg aatgctggtg aatcgtagcc
    21181 aggcggatgt cgacaccgca catcggctgg cggaagatat cctgcgcttc gcccgctgcc
    21241 gccagggtgg tgcactgacc tgacagaaac acagaaaaga agcgatttgc cgcaatctta
    21301 agcagttgaa tcgcttttac tgaaattagg ttgacgagat gtgcagatta cggtttaatg
    21361 cgccccgttg cccggatagc tcagtcggta gagcagggga ttgaaaatcc ccgtgtcctt
    21421 ggttcgattc cgagtccggg caccaaattc atataaacgg acctccacgg aggtccgttt
    21481 ttcgtttcag gacgccacga tttaagcgtt ctgcctccaa atcaattcta ccgaactcaa
    21541 ccagattctc cccacatcac cagcattttg cgggtatatc ccaattcggg aaaatttgtt
    21601 cctgagcgat agcactgact gacgtgaaat gtcgtgcggc cccgtgatgt tgttgaacgt
    21661 caaatgacgt catcaggagc gtaacgcatc cataaagcac aacaccggtc tgaacggcga
    21721 ctgatgagat tttctgaatg agaacaaaga gaaatgtatc agtccgcttg ctcatgcaaa
    21781 gactaacaat ccattaaaat agtaagcgct ccggacaatt ttccatggat tattttctac
    21841 acatttttct ttggcaaaga tgatgaattt tgatggtaag gaatattact tctggttctc
    21901 agtaaaatcc tttcgtaata ctatgtaatc aagaagttta tggctggtaa aaataacgtc
    21961 ttgcattcac caataacatg taaataaacc catctattga tggtaaaaat aggtcatggc
    22021 aattatcatt gcaccattcc cttttcgaat gagtttctat tatgcaacaa cctgtagttc
    22081 gcgttggcga atggcttgtt actccgtcca taaaccaaat tagccgcaat gggcgtcaac
    22141 ttacccttga gccgagatta attgatcttc tggttttctt tgctcaacac agtggcgaag
    22201 tacttagcag ggatgaactt atcgataatg tctggaagag aagtattgtc accaatcacg
    22261 ttgtgacgca gagtatctca gaactacgta agtcattaaa agataatgat gaagatagtc
    22321 ctgtctatat cgctactgta ccaaagcgcg gctataaatg aatggtgccg gttatctggt
    22381 acagcgaaga agagggagag gaaataatgc tatcttcgcc tccccctata ccagaggcgg
    22441 ttcctgacac aaattctccc tcccacagtc ttaccattca aaacaccgca acgccacctg
    22501 aacaatcccc agttaaaagc aaacgattca ctaccttttg ggtatggttt ttttcctgtt
    22561 gtcgttaggt atctgtgtag cactggtagc gttttcaact cttgatacac gtcttcctat
    22621 gagcaaatcg cgtattttgc tcaatccacg cgatattgac attaatatgg taaataaaag
    22681 ttgtaacagc tggagttccc cgtatcagct ctcttacgcg ataggcgtgg gtgatttggt
    22741 ggcgacatca cttaacacct tctccacctt tatggtgcat gacaaaatca actacaacat
    22801 tgatgaaccg agcagttccg gtaaaacatt atctattgcc tttgttaatc agcgccaata
    22861 ccgtgctcaa caatgcttta tgtcggtaaa attagtagac aatgcagatg gttcaaccat
    22921 gctggataaa cgttatgtca tcactaacgg taatcagctg gcgattcaaa atgatttact
    22981 ggagagttta tcaaaagcgt taaaccaacc gtggccacaa cgaatgcagg agacgctcca
    23041 gcaaattttg cctcatcgtg gtgcgttatt aactaatttt tatcaggctc atgattattt
    23101 actgcatggc gatgacaaat cattgaatcg tgccagtgaa ttattaggtg agattgttca
    23161 atcatcccca gaatttacct acgcgagagc agaaaaagca ttagttgata tcgtgcgcca
    23221 ttctcaacac cctttagatg aaaaacaatt agcagcactg aacacagaaa tagacaacat
    23281 tgttacactg ccggaattga acaacctgtc cattatatat caaataaaag cggtcagtgc
    23341 tctggtaaaa ggtaaaatag atgagtctta ccaggcgata aatactggca ttgatcttga
    23401 aatgtcctgg ctaaattatg tgttgcttgg caaggtttat gaaatgaagg ggatgaaccg
    23461 ggaagcagct gatgcatatc tcactgcctt taatttacgc ccaggggcaa acacccttta
    23521 ctggattgaa aatggtattt tccagacttc tgttccttat gttgtacctt atctcgacaa
    23581 atttctcgct tcagaataag taactccggg ttgatttatg ctctggaata tttgttgttg
    23641 agtttttgta tgttcccatt ggtataatat gttgcggcaa tttatttgcc gcataatttt
    23701 tattacataa atttaaccag agaatgtcat gcaatccatt gtaaacattg aatgtttatc
    23761 ttttcatgat atcaacttgc gatcctgatg tgttaataaa aaacctcaag ttctcactta
    23821 cagaaacttt tgtgttattt cacctaatct ttaggattaa tccttttttc gtgagtaatc
    23881 ttatcgccag tttggtctgg tcaggaaata gttatacatc atgacccgga ctccaaattc
    23941 aaaaatgaaa ttaggagaag agcatgagtt ctgccaagaa gatcgggcta tttgcctgta
    24001 ccggtgttgt tgccggtaat atgatgggga gcggtattgc attattacct gcgaacctag
    24061 caagtatcgg tggtattgct atctggggtt ggattatctc tattattggt gcaatgtcgc
    24121 tggcgtatgt atatgcccga ctggcaacaa aaaacccgca acaaggtggc ccaattgctt
    24181 atgccggaga aatttctcct gcatttggtt ttcagacagg tgttctttat taccatgcta
    24241 actggattgg taacctagcg attggtatta ccgctgtatc ttatctttcc accttcttcc
    24301 cagtattaaa tgatcctgtt ccggcgggta tcgcctgtat tgctatcgtc tgggtattta
    24361 cctttgtaaa tatgctcggc ggtacctggg taagccgttt aaccactatt ggtctggtgc
    24421 tggttcttat tcctgtggtg atgactgcta ttgttggctg gcattggttt gatgcggcaa
    24481 cttatgcagc taactggaat actgcggata ccactgatgg tcatgcgatc attaaaagta
    24541 ttctgctcta cctgtgggcc ttcgtgggtg ttgagtccgc agcagtaagt actggtatgg
    24601 ttaaaaaccc gaaacgtacc gttccgctgg caaccatgct gggtactggt ttagcaggta
    24661 ttgtttacat cgctgcgact caggtgcttt ccggtatgta tccgtcttct gtaatggcgg
    24721 cttccggtgc tccgtttgca atcagtgctt caactatcct cggtaactgg gctgcgccgc
    24781 tggtttctgc attcaccgcc tttgcgtgcc tgacttctct gggctcctgg atgatgttgg
    24841 taggccaggc aggtgtacgt gccgctaacg acggtaactt cccgaaagtt tatggtgaag
    24901 tcgacagcaa cggtattccg aaaaaaggtc tgct
//
LOCUS       DAESHI010000056        24493 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.056, whole genome shotgun sequence.
ACCESSION   DAESHI010000056 DAESHI010000000
VERSION     DAESHI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24493
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.056"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            142..489
                     /locus_tag="KW011_002356"
     CDS             142..489
                     /locus_tag="KW011_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 1B immunity protein"
                     /protein_id="HBH7660667.1"
                     /translation="MKLDISVKYLLKSLIPILIILTVFYLGWKDNQENARMFYAFIGC
                     IISAITFPFSMRIIQKMVIRFTGKEFWQKDFFTNPVGGSLTAIFELFCFVISVPVVAI
                     YLIFILCKALSGK"
     gene            complement(507..2387)
                     /locus_tag="KW011_002357"
     CDS             complement(507..2387)
                     /locus_tag="KW011_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 1B"
                     /protein_id="HBH7660668.1"
                     /translation="MSDPVRITNPGAESLGYDSDGHEIMAVDIYVNPPRVDVFHGTPP
                     AWSSFGNKTIWGGNEWVDDSPTRSDIEKRDKEITAYKNTLSAQQKENENKRTEAGKRL
                     SAAIAAREKDENTLKTLRAGNADAADITRQEFRLLQAELREYGFRTEIAGYDALRLHT
                     ESRMLFADADSLRISPREARSLIEQAEKRQKDAQNADKKAADMLAEYERRKGILDTRL
                     SELEKNGGAALAVLDAQQARLLGQQTRNDRAISEARNKLSSVTESLKTARNALTRAEQ
                     QLTQQKNTPDGKTIVSPEKFPGRSSTNHSIVVSGDPRFAGTIKITTSAVIDNRANLNY
                     LLTHSGLDYKRNILNDRNPVVTEDVEGDKKIYNAEVAEWDKLRQRLLDARNKITSAES
                     AINSARNNVSARTNEQKHANDALNALLKEKENIRSQLADINQKIAEEKRKRDEINMVK
                     DAIKLTSDFYRTIYDEFGKQASELAKELASVSQGKQIKSVDDALNAFDKFRNNLNKKY
                     NIQDRMAISKALEAINQVHMAENFKLFSKAFGFTGKVIDRYDVAVELQKAVKTDNWRP
                     FFVKLESLAAGRAASAVTAWAFSVMLGTPVGILGFAIIMAAVSALVNDKFIEQVNKLI
                     GI"
     gene            complement(2666..4012)
                     /locus_tag="KW011_002358"
     CDS             complement(2666..4012)
                     /locus_tag="KW011_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660669.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKKPATMAQHNLA
                     NDLLREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSFGASRHPVSTRINYRNFLPPDDSNSVIRLQTRSAYHNGERSSVRKKRPRLG
                     RLLLSLIEDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRSLSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFKKHMYGVFMHLRY"
     gene            4366..4962
                     /locus_tag="KW011_002359"
     CDS             4366..4962
                     /locus_tag="KW011_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proQ/FINO family protein"
                     /protein_id="HBH7660670.1"
                     /translation="MEKNQEERKIPVIVVKKRRTFSLPSLSEKTDIIESAFTEQAAES
                     AHAGINSSAVETHIPEAPARKKKKRRFPRPSHWTREYTHECVEKIKILFPHLRAEGGG
                     FIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGLDG
                     HPKGQVSASEAQSAGRRLVTLEQKWLRMQAQQENISGQ"
     gene            5018..5521
                     /locus_tag="KW011_002360"
     CDS             5018..5521
                     /locus_tag="KW011_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660671.1"
                     /translation="MSKSGAEKIQFILNNHEQAYRRLKPVYTFLELASWISVVLLIIY
                     LSGKNHVLTAAFLCFAWTAFWVLFLLHYFPKVFSILDRQSLLNLMEATKDSPDVKQEL
                     LNRLFSGKRMTGRDEMDICRLLRKNEEEKIRQSELNIIMNFIDEQQLTNSDIPKKSTV
                     PNEDKKA"
     gene            complement(5873..6154)
                     /locus_tag="KW011_002361"
     CDS             complement(5873..6154)
                     /locus_tag="KW011_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBH7660672.1"
                     /translation="MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLA
                     GQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            complement(6151..6420)
                     /locus_tag="KW011_002362"
     CDS             complement(6151..6420)
                     /locus_tag="KW011_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660673.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRDEMSRKVAGK"
     gene            complement(7331..8362)
                     /locus_tag="KW011_002363"
     CDS             complement(7331..8362)
                     /locus_tag="KW011_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBH7660674.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRAITFLVEPERK
                     RTKRRRGEHSTKPKCENPSWYRPERYKALSGQLGHAYNRLVKKDPVTGEQSLRMHMSL
                     HPFYVQKRTYAGRKYAFRPEKQRLLDAIWPVLVSFSDAGTHTVGMSVSRLAREISPKD
                     SKGKVIPELEVTVSRLSRLLAEQVRFGVLGVSEETLWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRLAIRELRQLE
                     LTLAAPPPH"
     gene            9335..9598
                     /locus_tag="KW011_002364"
     CDS             9335..9598
                     /locus_tag="KW011_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HBH7660675.1"
                     /translation="MPYHKDRNLYFLSSSMVAQAGQALAWPVSDDAGIPTPVWAITNE
                     RRNSVIAFLVVIGDLCYVTSTDTETPGHHSVGRHVSRAINKPA"
     gene            9516..9803
                     /locus_tag="KW011_002365"
     CDS             9516..9803
                     /locus_tag="KW011_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HBH7660676.1"
                     /translation="MSPQLIQKLPAITLLEGMFPELSTNQLKVCVFYAMGVPYDAIAQ
                     NCRLSPETVRTYLKRSLKNLNLEGYDALRSAVLMRTFVFMISNTAKENEKM"
     gene            10245..10778
                     /locus_tag="KW011_002366"
     CDS             10245..10778
                     /locus_tag="KW011_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /protein_id="HBH7660677.1"
                     /translation="MNIEHLNNRNWYLAQYNTAGKNRESLFSWLNEQNVVPWTPLITR
                     KIRRADSRCCYRERIFAIFPGYFFILANFDIHPVSALRRHSAFIDFVKFGGEIKPVNK
                     DIVDGLMKIYPDPVLNPGAREELNAASSIWLTKAQYQYLLRMENTLQPESRISLLLEL
                     VSNAEHHGFMERLVNIP"
     gene            11032..11715
                     /locus_tag="KW011_002367"
     CDS             11032..11715
                     /locus_tag="KW011_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="HBH7660678.1"
                     /translation="MTTTEHPVRQLLWCALNALKTAQENHNITSETGLRHYLLEWLSG
                     ASRHPEFRSLPGEIMALKALTEKDRNIPIIGTLNTLFLSSATVGECPLFRFRAALGKL
                     QKAGWRTYVCPWPERVFNESIERACSGKRHLLQLSRTEECFLPTGDMRAPVTLQLITP
                     LHRKTMQLLEAAELIFSDEGFQVVRGYEHQFGIERRETLFHTLFIGLPSLPEDVWGAT
                     QEQTTITLH"
     gene            11729..12295
                     /locus_tag="KW011_002368"
     CDS             11729..12295
                     /locus_tag="KW011_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HBH7660679.1"
                     /translation="MKLQYRIPLAIYSVWCIIAILLCKDGLYQMNIWTLVEILGMIAL
                     PLLVRPFFILLRIIFRKNSFKTKDMSDNHTVCVFLSALADTSSKKAIKKHFKQLLEKL
                     PPLLRQKKRIYMKSHLLTEARTQKLICSLRRKGLDVTAERRESAMNSVMFRILIVSRI
                     LSQWKVPHINPRCGIVILTLKNDSGNRE"
     gene            12563..12817
                     /locus_tag="KW011_002369"
     CDS             12563..12817
                     /locus_tag="KW011_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HBH7660680.1"
                     /translation="MPQQHPGRLQVLVVDTHCKRKLFSTKTQTDPDELARRFCTPDNC
                     LVVVLCNNRFLFRLERAPGSHCRWRKGSRSRHQHLQDWLS"
     gene            12860..13312
                     /locus_tag="KW011_002370"
     CDS             12860..13312
                     /locus_tag="KW011_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pili assembly chaperone"
                     /protein_id="HBH7660681.1"
                     /translation="MSFLFKLTIAILIVFAILFPILISSDNKLSALFWKIPKDFWVFL
                     IIMYGAIITVVFIESSEQARIRRQQYLQQYKMPNEIHENVVIENSIISRETTDDESDI
                     NTLYLDSFKSKNNCKSFETNSNLLDKNGKPVEIMVCDVQELHFFVPKN"
     gene            13986..15053
                     /gene="pilL"
                     /locus_tag="KW011_002371"
     CDS             13986..15053
                     /gene="pilL"
                     /locus_tag="KW011_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis lipoprotein PilL"
                     /protein_id="HBH7660682.1"
                     /translation="MKKNYLTLILPSLLSGCAGISGSNNVAPSDTPALVFVDGQISES
                     AGVLALTQQRLSPPKITPPPRVSVPETVKTAHPVSESRPLPSPTISQNFSRLIITGKP
                     GSAPVTLSPTARNRTVSQWIKTLLPEGWKFRQENAATPKLNTKLVSWSANDQWTRSLN
                     RLLEEQHLWGHLDWDNKTLTVTTSAAAPAQDTATTISPAAMVTTSTSPESPTTANSQN
                     KPRNPFRGNSVSSSTPAATPTPTGSTVKSIPLMTGTPVKPVSQGKEWRAPAGTTLREN
                     IIKWAEETKCESMASTHWMVIWPLSVTDYRLDAPLMFRGSFESAMEQVFELYRTAQKP
                     LYAQASRMQCIITVSDTRDGR"
     gene            15053..15490
                     /gene="pilM"
                     /locus_tag="KW011_002372"
     CDS             15053..15490
                     /gene="pilM"
                     /locus_tag="KW011_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HBH7660683.1"
                     /translation="MGWLVMAVGLTILIITGSYQNQKMSETTNAQQYASASVWASQIL
                     MIANRINDIRYVSGQQDGVISSDKLALPVTPDSRIKHQLQQGRLWVWMPEQPGLVETL
                     RSKSRGSALIGIFQNGQLTWLSGTATGLTPPAGITAGSVVYVN"
     gene            15504..17186
                     /locus_tag="KW011_002373"
     CDS             15504..17186
                     /locus_tag="KW011_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HBH7660684.1"
                     /translation="MKKSHQRSMKLAVLPCMIAVALSISGCTFSEINKMQKKAQEDSA
                     HAREKVSALSARKSQALTWLDNQWINPVPVAQVSREKKQTAPACYITQARKGEITLQE
                     LGQRITAVCGIPVIITPDAANSTLEGGATRQMTGTLPAPDENGRLPLSSLGSTTMTTS
                     TQPLTLNNLMWQGDINGLLDLMASRTGLYWRMDNGRIVFYLTETRTYPLHMLNTKTSS
                     SSSVSSGSTSTMGATGGQDNSASGDATSSQSTTVGQEYDLYEDIRKTIEAMLTPEKGR
                     YWLSASSSTLTVTDTPAVQEAVARYVDEQNSIMNRQVALNVQVLSVSNTRNEQFGLDW
                     NLVYKSLHSAGATLNNASGDFTGATSAGVSILDTATGNAAKFSGSSLLIKALSEQGDV
                     SVVTSQESTVTNLTPVPIQMADQTVYVAQSATTTTTDVGATTTLTPGMITTGFNMTLL
                     PLIQKTGNLQLQMNFNLSDPPTIRSFTSKDGNSYIEMPYTKLRSLSQKVNLKEGQSLV
                     VTGFDQNNTTTSKAGTFTPANPLFGGSQTGKNERSTLVIIITPTFPSGGNNG"
     gene            17179..18474
                     /gene="pilO2"
                     /locus_tag="KW011_002374"
     CDS             17179..18474
                     /gene="pilO2"
                     /locus_tag="KW011_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HBH7660685.1"
                     /translation="MADEDINPVVLLADPKVNHRVWAACLKWSPVVKKQRVPSHQKHK
                     PHVKSRRLTSLKVTVGSRSSRGKISRITGTGILARPERNHYFSLALAFCSWVRNGYGV
                     FRYSDKELLFLASINGQPAVMADLSGNDADVAQKVSLFLTMNEEPPEKWQVVSPLEHP
                     DNWESIITRLSSADLRRCKLTVGNRSKFTLPAVLFLVAASAGTVFWMTQPEPDVVPTP
                     EEIAARARLQFKKPDPPPELPHPWASQPVISDFLKACADLRKPSPVALEGWKLTGGTC
                     TPETFTLIYERQPGGTIEGFLARSKEIFNVIPDFNLKDGARLASVTRPLPSLPRRDEA
                     VPTPSEQLMRVFTWFQKKQLTPAINEIAIPEPLPGNDGEPAPVQKWKEYQFSLSTPVN
                     PDELFPLFQDTGVRLSNIHFELNGGTFSYSSEGHIYASR"
     gene            18461..18913
                     /gene="pilP"
                     /locus_tag="KW011_002375"
     CDS             18461..18913
                     /gene="pilP"
                     /locus_tag="KW011_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HBH7660686.1"
                     /translation="MRPGKLLIIPSIFFFSGFSFATTQPLVTIGELEAQQNRNILLQA
                     KVQGAQLQKQLEESDVTSSSETVSGFSGVTASLPSVSEQPTSKNRKELPVIMEINGKD
                     KRLNAVLRMADGRQTSVTTGSQLPGTSVTVKSISLSGVTLSDGTTLTF"
     gene            18924..20477
                     /gene="tadA"
                     /locus_tag="KW011_002376"
     CDS             18924..20477
                     /gene="tadA"
                     /locus_tag="KW011_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBH7660687.1"
                     /translation="MDKYNLKDALLFISRGDTHEILIETNQRTRPDVQSNLQELLKLY
                     PDINPKVVSLSELQEAGQDEKNRGPKERIIHLKDLADVSESEKKVLSYFETARKLGAS
                     DIHFLISESIFKVRMRIFGELQTVDEDQPALGYSLCATAILSMADVTETSFFPQREQD
                     ARLSPQLMRKIGIFGARYSHRPTGDGLIAVMRLIPDDGDKVPTFKQLGFIPEQIRLLN
                     IMLRRPEGKIVLSGPTGSGKSTTLRSACRVYLDDNQGRHLLTIEDPLEGQILGATQTP
                     IICDKSDEDAVKLAWSRAISSAMRLDPDAIMEGEMRDLISMMSTTYAAQTGHIVLTTL
                     HTNSALGIPERMITMGMNADLICDAQLLIGMISQRLVPTLCPSCRIPWEKRAPELSDD
                     ERDYLERHCNKDSLCSTDNIWFRNHHGCSECNHDVIINGRKRGEIGKGLTGRTVIAEV
                     IEPDNRLFQILKTRGKVAARKYWLENMKGISRVEHLLRRINEGLVDPLEADRIIPLDE
                     DERLSIDDV"
     gene            20490..21587
                     /locus_tag="KW011_002377"
     CDS             20490..21587
                     /locus_tag="KW011_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001699973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBH7660688.1"
                     /translation="MREMNFSQRLRRFIVRKTFSAPYRVQFYEALRFLLENKQPLKTA
                     LEQMRDAWTDFGRKWHPFAELATDCIESLRENSGENSLEYTLSLWVPQEEAAVISAGI
                     RSGSIVDALQFATTLTDAKEQIHQAIWQMAIYPVGLLIMMTGTLYVLNTELIPELSKI
                     SSPDSWSGALGFLYGLSVFVDNYGAICAVLFAVITGLISWSLPNWKSPDSVRTFADKI
                     MPWSIYQDIQGATFLLNMAALLKAKMTTLNSLNILQEFASPWLSTRLDSIIYRVRQGD
                     HLGLALRQCGYQFPSREAANFLSLLQGDGATELISNYGQRWLSQTLQRVKKRANVIRL
                     IMLIFLVMSLMLLVFAIMDIQSISDNSMGNF"
     gene            21605..22219
                     /locus_tag="KW011_002378"
     CDS             21605..22219
                     /locus_tag="KW011_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilX"
                     /protein_id="HBH7660689.1"
                     /translation="MLVENINTTLTGNNKKNEPHDKGWAILEQGTIALVVLFVIVVVL
                     GSLYALRTRANVATETANIQTIITSAQSLLKGSDGYTFTSSAKMTGALIQMGVIPSGM
                     TVQGDKTSGTATLYNAWGGAVTVAPASTSGFNNGFTVTYDKVPQDACIQIATRISKTG
                     LTNGITLNSTAHSDGKVTTEEASTQCKADNGSTGTNKLIFTING"
     gene            22229..22789
                     /locus_tag="KW011_002379"
     CDS             22229..22789
                     /locus_tag="KW011_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBH7660690.1"
                     /translation="MSGGTVPLRLISLILLFSLIPPARASYVAPKQIWTNKWDNCFAA
                     AGARYQIEPLLLKAISAGESSLRPGAININKDKKTGKASSTDYGLMQINSTHIPKLIN
                     MGVIKKSEDLITKPCLNIHIGSWILARHFQICGVSWNCLGSYNAGFRKDRHETREQYA
                     NKIWRIYRDMKGICLPGQGGRQCRQS"
     gene            22774..23430
                     /locus_tag="KW011_002380"
     CDS             22774..23430
                     /locus_tag="KW011_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBH7660691.1"
                     /translation="MQTIVGISLSPVYILPLMFTFSIIGRTLLFRVRYFLSDTGYLWY
                     KTHPAVLSGIWLYSIAVALIILSSSPLLYRIHAVLILSFILQMAVTDALTGLLPGTFT
                     RRFLIAGMLSQITTDIWWFRTTEFATAAIVLFCLHKLVNRHRLNIGTGDLWLIAGITA
                     WSGLYNAIWCVLLGTGGFVLWHSTWCIKGHKEGPLGPWLCFGHVLLLLDNLYQPLWVI
                     "
     gene            23430..>24493
                     /gene="pilV"
                     /locus_tag="KW011_002381"
     CDS             23430..>24493
                     /gene="pilV"
                     /locus_tag="KW011_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HBH7660692.1"
                     /translation="MKKYDRGWASLETGAALLIVMLLIAWGAGIWQDYIQTKGWQTEA
                     RLVSNWTSAARSYIGKNYTTLQGSSTTTTPAVITTTMLKNTGFLSSGFTETNSEGQRL
                     QAYVVRNAQNPELLQAMVVSSGGTPYPVKALIQMAKDITTGLGGYIQDGKTATGALRS
                     WSVALSNYGAKSGNGHIAVLLSTDELSGAAEDTDRLYRFQVNGRPDLNKMHTAIDMGS
                     NNLNNVGAVNAQTGNFSGNVNGVNGTFSGQVKGNSGNFDVNVTAGGDIRSNNGWLITR
                     NSKGWLNETHGGGFYMSDGSWVRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLERT
                     AVAGASCSPNGLVGRDNTGA"
BASE COUNT         6722 a         5840 c         5488 g         6443 t
ORIGIN      
        1 gcttttttct gctcaaaggt cacaggagca cctccgttcc atgatgtaaa cgtgacacag
       61 cttattgtta ttgattatta ttatcatgct gtgattatgt tatcacgcac cggtacacga
      121 taacaataaa ggagaaacca gatgaaactg gatatatctg taaagtattt actgaaaagc
      181 ctgataccaa tcctcattat tcttacagtt ttttatctgg gatggaaaga taaccaggaa
      241 aatgcaagaa tgttttatgc gttcatcgga tgcattatca gtgccattac ttttcctttt
      301 tcaatgagga taatacagaa aatggtaata aggtttacag ggaaagaatt ctggcaaaaa
      361 gacttcttta caaatccagt tggcggaagc ttaactgcaa tatttgaatt attctgtttc
      421 gttatatcag ttcctgtggt tgccatttac ttaattttta tactctgcaa agccctttca
      481 ggaaaatgaa agggcatcat tatgtgtcag ataccaataa gtttattgac ctgctcaata
      541 aacttatcat taacaagcgc actcacagcc gccataataa ttgcaaaacc cagaatacct
      601 acaggggttc ccagcatgac ggaaaacgcc catgctgtaa ctgctgaagc agctcttcct
      661 gctgccagtg attcaagttt tacaaaaaat ggacgccagt tgtccgtttt tacagccttt
      721 tgtaactcca cagcaacatc ataacgatca ataacttttc cggtaaaacc aaatgcctta
      781 ctgaacagct taaaattctc cgccatatgg acctgattaa tagcttccag ggctttagaa
      841 atggccatgc gatcttgtat gttatatttc ttgttcagat tattacggaa tttatcaaaa
      901 gcgttcagtg catcatccac actcttaatc tgtttccctt gagatacaga agccagctcc
      961 ttagcaagtt cggatgcttg tttaccgaac tcatcatata tcgttctgta gaaatcagag
     1021 gtgagtttta tggcatcctt taccatattt atttcatccc tttttctttt ctcttcagct
     1081 attttctgat tgatgtcagc aagctggcta cggatattct ctttttcctt caacagagca
     1141 ttaagagcgt catttgcatg cttttgttca tttgttctgg cactgacgtt atttctcgcc
     1201 gaatttatcg cagattcagc agaggtgatt ttatttctgg catcaagcaa tcgttgccgt
     1261 aacttatccc attcagcaac ttcagcatta taaattttct tgtcaccttc cacatcctct
     1321 gtcaccaccg gattccggtc attcagaata ttgcgtttat agtccagacc ggaatgggtc
     1381 agaagataat tcaggtttgc acggttatcg atgaccgcgc tggttgtgat ttttatcgta
     1441 ccggcaaacc tcggatcacc actcacaaca atagaatgat ttgttgatga acgccccggg
     1501 aatttttcag gggaaactat cgttttgccg tcaggcgtgt ttttctgttg cgtcagctgt
     1561 tgttcagctc tggttaatgc attacgggcc gtcttaagcg attccgtcac cgaactgagt
     1621 ttattccggg cctctgaaat ggccctgtca ttccgtgtct gctgcccgag cagacgggcc
     1681 tgttgtgcat caagaacggc aagggctgcc ccgccatttt tttccagctc tgacaaccgc
     1741 gtgtccagaa taccttttct gcgctcgtat tcagcaagca tatcagcggc cttcttgtct
     1801 gcgttctgcg catccttctg ccgtttttca gcctgttcga ttaacgacct ggcctcgcgg
     1861 ggagatatac gaagagaatc agcatcagca aacagcatcc ggctctctgt atgcagccgg
     1921 agggcatcat atccggcgat ttcagtacgg aatccgtatt ctctcagctc tgcctgcagg
     1981 agtctgaact cctgtcgtgt aatatcagcg gcatctgcgt ttccggcacg gagtgttttc
     2041 agtgtgtttt catctttttc ccttgcagca attgccgcag aaaggcgttt tccagcttca
     2101 gtacgcttat tctcattctc tttctgctgc gcgctgagcg tgtttttgta cgctgtgatt
     2161 tccttgtccc ttttttcgat atcacttcgg gttggggaat catcgaccca ctcgtttcca
     2221 ccccagatgg ttttgttccc gaaggaactc catgcaggcg gggtaccatg aaagacatcg
     2281 acacgtggag ggtttacata aatatcaacg gccataattt catggccatc tgaatcatat
     2341 cccagcgatt ctgcaccggg atttgtaata cgtacagggt cagacattcg gtatctcctt
     2401 catcctctga ggaaaacaaa tgaattaact gcttacgtat atggatacat atacagtact
     2461 aatttattaa ggagaaaatg gcatgtcaag atttaatgat agtaattatc attaacatga
     2521 agtggtgtta taccgttcag aaagcctgct gacactgtgg cagcatcaca gtctgggtat
     2581 tctctggtgc aatacaaaaa ccggactttc ccgtaacaac agacaatgtt acgggaaaag
     2641 ttacagaact gagccacaaa atatctcagt acctgagatg cataaaaaca ccgtacatat
     2701 gtttcttaaa agggggatga tcgttatcca gccatttcgg tggatcggta tgcagaacac
     2761 caatctgctt catgccggta tgctgcctgg attttctcgc gcagtaatca ctgtcttcat
     2821 atcccatcgt ttcgatgacc actctggccg tctttccgtc aggagccctt gctgtgacaa
     2881 tgaaatccgg cagtacatat ttcttttcac cattaacata aacttctgta tcaaacaggg
     2941 gcctttccag acttaattcc ggcgtacctg gctttctttt aagccagctg gccacattca
     3001 gaagagcagt gagcgtgttt cgttccagtt tgctgtccac aggcaccgga catgtcagtt
     3061 gataaagagc atgagcatac ccttcactgc agatgattct gccatcggca ctccggcaaa
     3121 aagcaagaat aacccagtaa gggggacgta ttccgccctg cgcttcacca ttaatactga
     3181 tcccccgcac aggcctgatg acctgaatat taccgttcgc ccagtgaatt tccacagcat
     3241 cacgacagat gtgctgcgcc ataaaaatct gaaagaacat atgttttctt ctggcaggcc
     3301 agtgtcgttc tgaattctcc agcgtttcca ttaaccgctc ctgagacctt tcagacattc
     3361 ctggacggaa atggataatt tcagataagg atcttccccg aatatactcc tgctgtagtg
     3421 taacctgccg tatggcatca agacactcac gataattagt ccggatacgg ggattagcca
     3481 gggaatccag tttattcagc cctgcatctt caataagcga caggagtaaa cggccaaggc
     3541 gcggacgttt cttgcgaacg gaggagcgct caccattatg gtaagcagag cgggtctgca
     3601 gtctgataac agaattactg tcatcaggtg gtaaaaagtt tctgtaattt atacgggtgc
     3661 taacgggatg ccggcttgct ccgaaggatg tttcctcttc atttccggat aactgacgat
     3721 acatggggca ttcaggtgca tgctcatgat taaaactgga cagataaaga gtacccgtat
     3781 tgtttttaag agcagcaaaa ttcatggccg gcgaatctcc cggaatacag tcacactgta
     3841 accatgcatc atactcacgc atttcccgga gaagatcatt ggccaggtta tgttgtgcca
     3901 tggttgctgg ctttttcagt ccacagaccg tctttactgc agtctcttcc tcactgctaa
     3961 gctcccgaat cactttgccc cggggattct tctttacaag tatcattggc ataatgaaag
     4021 cactccttat gtacatgctt aaacagtaag taaaatatac aaagaacacc ccatgaaaca
     4081 aaggcagaga tgaactgtgc gccccgatat cttcttctga catcctttca aatgaattgt
     4141 ttttatttat atcatgacaa cattaacgtg agagaatata taaaccatgt ttcggtcagg
     4201 gttcagtcga atatcaattc gactgtcacg ccataactgt taccacctcg agtataaata
     4261 ttcagagaac ggacttatta accatgaaaa atcacctgac ggaaaattta ctaatttatg
     4321 gattgtgata ttattaaaga gtaaaaccag ccagtcgagg tatttatgga aaaaaatcag
     4381 gaagaaagga aaatccccgt cattgtagtg aaaaaacgtc gtaccttttc gttaccttcc
     4441 ttatccgaaa aaacagatat tatcgaatct gcatttactg aacaggcagc agaatcagcc
     4501 cacgccggaa taaattcatc tgccgtcgaa acacatatcc ccgaagcacc agccaggaag
     4561 aagaaaaaac gccgtttccc acgtccctcc cactggacca gagagtatac tcacgagtgc
     4621 gtggaaaaaa taaagatcct gtttcctcat cttcgtgctg aaggtggtgg atttattccc
     4681 ctgaaaattg gcataaacaa tgatatttca gcatttctgg cagaacaccc ggagacagaa
     4741 ctgaccatgg acgaatggct ctgtgcagtg tcctgcatca cctcacgccg tgtatatctg
     4801 cagcggacag ctgtcgccgg cgtcccacgt tatggtctgg atggtcaccc aaaagggcag
     4861 gtctcagcat ctgaagcaca gagtgcaggt cgcagactgg tgacattaga acaaaagtgg
     4921 ctcaggatgc aggcacagca ggagaatatt tctgggcaat aaacactcct gataacagct
     4981 ggacattacg tatcagaaaa atgggaagag aattattatg agtaaatcag gtgcagaaaa
     5041 aattcaattc atcctgaata atcatgaaca ggcatacaga cgcctgaagc ctgtatatac
     5101 ttttttagaa ctggcatcct ggatttcagt tgttctgctg attatatacc tatcaggcaa
     5161 gaatcacgtg cttacagcag cctttctctg ctttgcatgg acagcattct gggttctttt
     5221 ccttttacat tatttcccaa aagttttctc tattctcgat cgtcagagtc tgctcaacct
     5281 catggaggcc accaaagact caccagatgt aaagcaggag ctgcttaacc gccttttttc
     5341 cggaaaaaga atgaccgggc gggatgaaat ggatatctgc cgtcttctga gaaaaaatga
     5401 agaagaaaaa atccgtcaga gtgaactgaa tatcatcatg aattttattg atgaacagca
     5461 gttaacgaat agtgatattc cgaagaaaag tacggtccca aatgaagata aaaaggcata
     5521 agatgatgat atgaaattaa taaccaggca agcagaggat ggcatggttt catcctaaga
     5581 ttgatagggg ttaacctgtc agataaaagg actgagttta cacaacgtat cttgttgacg
     5641 cgcaaaattt cgggttcggt tatgaaaaag taacatattc tatgctggaa cagagcaatt
     5701 ttgcattctc cgtccaggag tttgagggga aagtagtcag tgttttacgg tcagtcaggg
     5761 aaagtgtgca atgataaata acacgaaagg aatcgtagga ggtaacttaa caataactaa
     5821 gcttcttagc gtacgttttc gttccattgg ccctcaaacc ccgacagccc ctctatggcc
     5881 acttctgtgc agtatgcaat acgcgcagga tatacacttt tccccactgg ctgtcgacct
     5941 cataaaccag aacaaaatgc gggtgaataa ccagttcccg ggtaccttcc acacgaccgt
     6001 tgcggccttt atacggctgc ccggccaggc tgctggctga ataaccaatc cgttcgtcca
     6061 gttcaatggc ggccatcagg ttctgctccg caatgtactc acgaatgcgc ttcctgtcct
     6121 ggctggccag catggtccag aatatctcca tcatttaccg gcgaccttcc tgctcatttc
     6181 atcccgccat gctgcagcag aggattcgac catgtcatgg ggaagcacat cgccgtgctc
     6241 gagttgctga cgtcctgccg caacctggtc gcggaaccag gtattatgct gccgttcaac
     6301 ggtctggcgc ataaaatccc ggattaactg agagccatta cggtccatgc tttttgcagc
     6361 ggccataaaa gcatccttca gttcagcgtc gattcttaaa ctcatattaa cctgtgccat
     6421 ttatcacctc gtcattacca cgtatataca cagtatataa cgattcaaat atcgtcacca
     6481 ggatatgccg cgccagcggc atggaaggcg gcactccgtt gtagcatatg gcacaggagt
     6541 aaaaccgccg aagcccggcg taagccggta cggattgaca gatttcacct tacccatccc
     6601 cggccctgct aagccatacc cgctttcagc catgacagag cttttgtgcg cggtcggagt
     6661 ggtcccgacg agggtttacc cgaagtcggg gcgtatctcc gcgttagcgg gccgtcaggg
     6721 ccgcttacga gcgtgtactg aggacttcca gccagaagac tggcagcgat gatgtcgtgt
     6781 agttacaaca ttcataatta aaagcgatgt tgttcccgcc cttcgggcgg gggcggcctc
     6841 gcttttcagt tatgaggatg agcacggtgc tgcgtttgtg tggtgttttt tattcctgat
     6901 gtctttttgc tgtggtttca gaatggtgag gttcggggcc cgcgcgttag cgtgggaggt
     6961 acttttaaat tctgagcggc gcggccttgg ccgctgcaag cgaaggcctc tttgtatttg
     7021 ttcacagtgg cctcagagat aaagattttc cctgcggcga agcaacgcag gggattaaat
     7081 aatggggtta agactatatt gcaggtttgc tgttttaagc ttgcagcttc ttaaagttct
     7141 tagtttgcgg atttatgtgg ataactgaaa caacgtggcc acgttctgat gactgatgcg
     7201 tggttttatg gacagatatg cccctctgag gggatttaag atgattactg cggattttgg
     7261 gctaagcgga agcagcgccg aaagccccgg aggggctggc gatgattcag tgctgaggga
     7321 atggtactgt ctagtgcggt ggcggggcag ccagcgtcag ttcaagctga cgcaactccc
     7381 tgatggccag tcgcttcaga tggtcatcgg aacaaaaata ggcttcatcc ggcggcagac
     7441 gcttcctgag atactctgcc atctcatgaa tctgctggtc ggcaggcagt ttcttcaggc
     7501 gtctggcgcg cttactggct gccgcttttg cacgacggtg tttcagtgca tcctggctgc
     7561 gctgcagata ccagcgtttt ctggccgcat gtacggagat atcttcatcc tcacgcagaa
     7621 caccttcccg aatgagctgc tggcggattt cactttcacg cagtcgtttc tgctgctgtt
     7681 cgtgaagttt taccatgtcg acgcccagca tctgccagcc tgccggtgtt atccagacgt
     7741 aacgtggcag acgctggcgg gtttcacggt cccacagggt ttcctctgaa acacccagta
     7801 caccaaaacg tacctgttcg gccagcaaac gggaaaggcg ggagaccgtc acttccagtt
     7861 ccggaataac cttccccttg ctgtctttcg ggctgatttc tctggccaga cgggaaacac
     7921 tcatgcctac ggtatgtgtg cccgcatcac tgaagctgac cagaaccggc cagatggcat
     7981 cgaggaggcg ttgtttttcc ggacggaaag catatttgcg accggcatac gttcgtttct
     8041 gcacgtaaaa aggatgcaga gacatgtgca tgcgcaggct ctgctcgccg gtcaccgggt
     8101 cctttttcac cagacggttg taggcgtgcc cgagctgccc gctcagcgcc ttatagcgct
     8161 caggacgata ccaggacgga ttttcgcatt tgggtttggt ggagtgctca ccacgacgac
     8221 gcttcgtgcg cttcctttcc ggttcaacca ggaaagtgat cgcccttccc gcttcatagt
     8281 cttcccagaa acggatctgc tcttcgggtg ctaactgact ccagtgtact gcgttatatg
     8341 gagtattctt cagacctgcc acaagctgtc gcttatggcg gagcgattgt gccgtgtatt
     8401 tatacactgc acagggttga atctctggta cggtttcatt atactttacc cgtattctgt
     8461 gaggccccca ctatctttcg tcgttccgcc aagttcgtaa gaaagatagt gggggttttc
     8521 cttttatggc ttccggaaca tcttcagtct tctgacttac tgcccgtaca tgccatctct
     8581 cctgaatgcc ttatggtgca ttctgccgtc cggctttcgc cgctttgctc cacagagtgc
     8641 caccctctgc acagctaata acagccgaaa tatacccgct ttacgttaat ttgaaaagca
     8701 ttacaacgat ctcttctctt ccagtgcctc ctcactgata cctctaataa gttaatcttg
     8761 acctgcgtca aaaaaataaa atctattttt aaaagcagat ttttctgctt tagatctttc
     8821 ttttggatta aaagatcgac ttttgtccta ctcacgatca tttatcttac aaccgatcta
     8881 cctaacagaa taatattctg ttttaatgca attaaacgat ctaaataacc tttaaaaaat
     8941 cattttgtag atcattcgat cgtaaataga atgattctta gatgataaaa ccatttgtta
     9001 cacaacccgc caattgtgta acaacagcct ctacaagagc atatttgttc atatgttcgt
     9061 cagaactgcg gattgtctgg acgagggtca gacaccgttt cgtgcatatg gaccagcatg
     9121 ttcgaacgcc acaaagcaga agcatcatgc ctggatcgct tcggcgcgga gcgttaagtt
     9181 ttgacgcttt tttgtatgtt ttttaagctg ttagttgact cggtgcgtta tactaagtac
     9241 cctttactgg tcacaggcaa aactgtgatc gggcgtagga acccgcgtag tcatcgtcgg
     9301 cgcagaagtt aatttcttca tttggcgtcg ttttttgcca taccataaag acaggaactt
     9361 gtatttcttg tcgtcatcta tggtagctca ggcggggcag gcattagcct ggccggtttc
     9421 cgatgacgcc ggtattccta cccccgtctg ggctatcacc aatgagcgta ggaactcggt
     9481 gatagcgttt ttagttgtca tcggagacct ttgctatgtc acctcaactg atacagaaac
     9541 tcccggccat cactctgttg gaaggcatgt ttcccgagct atcaacaaac cagcttaaag
     9601 tctgcgtttt ttacgcaatg ggtgttcctt acgacgccat tgctcagaac tgtcggcttt
     9661 ctcccgaaac ggtacgaacc tacctgaagc gaagcctgaa gaatttaaat ctggaaggat
     9721 atgatgcact tcgctctgct gtgctgatgc gaactttcgt tttcatgatt agcaatacag
     9781 ctaaagaaaa cgaaaaaatg tgaacaggtg tccccattaa aggggacact ttcatggaaa
     9841 ttgcattttg cactttacag gcgatcccaa attgataaac ataaacacca agcacaagtt
     9901 actttaataa agcaaatcac ttcagacggt gtcccagtta tttgtatctg gctcatgccc
     9961 gatatatggc gaccttactc tgaactgacg tgctgatgtt ttatttcgcg tatcacaata
    10021 ctgtgccact gtattgctga tattagtgct tagccgttct ttatctgtga gttcgccaaa
    10081 tcaccgatac agaacgtact agagtctgct ggttatatgc ctgtcagaac tgcagtttat
    10141 ccagatatgg gctggacact gcttcagaca tatgatttag catattcgcc tgacatcgca
    10201 aaatcgtcca tcatggatcg gttttgcgcg gagcgttgaa cttcgtgaat atagaacacc
    10261 tgaataaccg gaactggtat cttgcgcaat ataatacagc cggcaaaaac agagaatctt
    10321 tattctcatg gctgaatgag caaaacgtcg taccatggac tccattaata acaagaaaga
    10381 taagacgggc agactccagg tgctgttacc gtgaaagaat attcgctatt tttccagggt
    10441 acttttttat tctggctaat tttgacattc acccagtatc agcattacgt cgacatagcg
    10501 ccttcattga tttcgtgaag tttggcggtg aaataaaacc cgtcaataaa gatattgttg
    10561 atggattaat gaagatttac ccggaccctg tgctaaatcc gggagccagg gaagagctga
    10621 atgcggcttc cagcatatgg ctgacaaaag cacaatatca gtatctcctc agaatggaaa
    10681 atactctcca gcctgagtcg aggatatccc tgctcctgga actggtatca aatgccgaac
    10741 atcacggctt tatggagcgc cttgtaaaca tcccatagcc agtatttccc ggccttcggg
    10801 ccgggctttt ttcaattcaa gagaatattc cggagtgaag cctgcacgaa gccatcccca
    10861 ccatggactc cccctgtctt ttgatggaac cgtgtatttc ttaatggcga aagaaataaa
    10921 aggcttcacc ccagagtatt gattcgtttt atcaaaggcc ggcccgtgcc atcacgtgtg
    10981 gcggagagaa cggataccgg tcttcttttc atctcacagt aggacattcg catgaccacg
    11041 acagaacatc ccgtgcgcca gttgctctgg tgtgcactga atgccctgaa aacggcacag
    11101 gaaaaccaca atataaccag tgaaaccgga ttacggcatt acctgcttga atggctttct
    11161 ggtgccagca gacatcctga gttccgtagt ctccctggcg aaatcatggc gttaaaagca
    11221 ttgaccgaaa aagatcgcaa tattcccatc atcggaacgc tgaatacgct ctttctcagc
    11281 tctgccactg tcggggaatg tccgttgttt cgttttcggg ctgcactggg taaattacag
    11341 aaagcaggct ggcgcacata tgtctgccca tggcctgaga gggtgttcaa tgaatccatt
    11401 gaacgggcct gttccgggaa acgtcatctg ctacaactaa gtcgcaccga agagtgtttt
    11461 ctaccgacag gcgatatgag ggcaccagtt acgttgcagc tgatcactcc actacaccgc
    11521 aaaactatgc agcttctgga agcggcagag ttaatattct ccgacgaagg atttcaggtg
    11581 gtgagaggat acgaacatca attcgggatc gaacgcagag aaaccctctt tcacacctta
    11641 tttatcgggc tgcccagcct gccagaagat gtctggggtg caacacagga gcagacgact
    11701 atcacattac actgaggaag agcaatacat gaaactccaa tatcgtattc cattagctat
    11761 atatagcgtc tggtgcatta tagcgatttt gctctgcaag gatggcttgt accagatgaa
    11821 catatggacg ctagtagaga tattggggat gatagctctc ccgctgttag tccgtccgtt
    11881 tttcattctg cttagaataa tatttaggaa aaatagtttt aaaaccaaag acatgtctga
    11941 caatcacaca gtatgtgttt tcctgagcgc actcgcagat acttcctcaa agaaggcgat
    12001 aaaaaaacac ttcaagcaat tactcgaaaa gctgcccccc ttattacgcc agaagaaacg
    12061 tatctacatg aaatcccatc ttctgactga agccaggaca caaaagctga tttgctctct
    12121 gcgccgaaaa gggctggatg taacagcgga acgaagggaa tccgccatga atagcgtcat
    12181 gttccgcatc ctcatcgtca gcagaatctt atctcagtgg aaagtgcctc acataaatcc
    12241 ccgctgtggt attgtcattc tgaccctgaa aaacgattct ggaaacaggg aataacagtt
    12301 ataagagcac cacgatttcc gacaggaaaa catacatgaa aatcaacgta gatgaccctg
    12361 gcaaaaagag cttcgctctc attacgcctc acgtgcgtta attcccgaca caccccgtga
    12421 cccgtcttcg cagcctgtac caccctttct gccgttcccg ggattctttc tgtcactact
    12481 cgcttgtgcc gttttttcgc aaccggtacg tgcagttacg acggtacagc tgcttctttt
    12541 cccgttccga aaggagtctt tgatgccaca gcaacatccc ggacgactac aggtactggt
    12601 cgtcgacacg cactgcaaac gcaagctgtt ttcaacgaaa acacagacag atcccgatga
    12661 gcttgcccga cgcttctgca cgccggataa ctgccttgtg gtggtactat gcaacaaccg
    12721 atttctgttc cgcctggaac gtgcacccgg tagccactgc cgctggcgca agggtagccg
    12781 ctcccgccat cagcacctgc aggactggct gtcgtaaaaa caacaaaaac aaaagacctc
    12841 gtcaaaacag gaggggacca tgtcgttttt atttaaatta accatcgcga ttttgattgt
    12901 ctttgcaatc ctttttccga ttttaatttc atcagataac aaactgtccg cacttttttg
    12961 gaagatacca aaagatttct gggttttcct gattatcatg tacggggcaa tcatcacggt
    13021 ggtgttcata gaaagcagtg aacaggcacg catccgccgc caacaatatt tgcaacagta
    13081 caaaatgcca aatgagatac atgaaaatgt ggtgatagaa aacagtatta tttcaagaga
    13141 aacaacagat gacgaatctg acattaacac cctgtacctt gattcattta aaagtaaaaa
    13201 caactgcaaa tcttttgaaa ccaattcaaa tttactggat aaaaatggta agcctgtgga
    13261 aataatggtg tgtgatgttc aggaactgca ttttttcgtt ccaaagaatt aaacagttaa
    13321 tcccccttac tctgttatct tagtcgccgg catcaactaa catgaaaaat aataacgtac
    13381 cattatatct atggttactt tggtgtacgt tttgcctgta catcactacc ccgtggcaat
    13441 acttccgtta tctatacact gaccacatat cactatctta tatattatat ttctgtatag
    13501 tggttattat ctgcttttcc ccactacttt tcagatgcgt gcttcttatc gtaaggtctg
    13561 tatctaaata caacttcaca ttctttgcga aaaaaaatgg cagtaaagaa tcaagcatac
    13621 atcttagccc ctatgccgat acacgttgcc cggagtcaat gaggaaacac tgggatgtgt
    13681 tactttctct gatatcaaaa gcaattaata atgatgaaac cattgttatg cattcacatc
    13741 tctttactcc tgcaagaata aataaactta taaagagact ggaatcttat ggtttgaatt
    13801 tctctgttat tacatgccca cggaaaacaa gtctgtatga aagattatca attccttttg
    13861 tatatctctt atttcagtgg tctctcccgc atttgcattc ggacggaatg acagtcgtca
    13921 ttcgtcctct ccagccgcct cacaccaatt aacaaacacg tatatacgaa cacggagtct
    13981 ccgttatgaa aaaaaactat ctgacactta tactgccgtc attattgtcc ggctgtgccg
    14041 gaatttccgg cagcaataac gtggcaccat cagatacgcc tgctctggtt tttgttgacg
    14101 gacaaatcag tgagagtgct ggcgttctgg cacttacgca acaacgtctt tctccaccta
    14161 aaataactcc ccccccgaga gtcagcgtgc cagagacagt aaaaacagca catccagtca
    14221 gcgaatcccg accgttacct tcaccaacaa taagtcagaa tttctcccgg ttaatcatta
    14281 caggcaagcc tggtagcgcc cctgtaacac tatcgccgac ggcccgtaac cggaccgtca
    14341 gtcagtggat aaaaacatta ctaccggaag gatggaaatt cagacaagag aatgcagcca
    14401 cgcccaagct gaataccaaa ctggtttcat ggtctgccaa tgatcagtgg acccgttctc
    14461 tgaatcggtt gctggaagaa caacacctat ggggccatct ggactgggat aataaaacgt
    14521 tgaccgtcac aacatcagct gctgcaccgg cacaggacac ggcaaccact atctcgccag
    14581 cagccatggt aaccacatcc acttcccctg aatctccaac aacagcaaat agccagaaca
    14641 aaccacgaaa tccattcaga ggaaacagcg tatcttcgtc aacccccgcc gccaccccga
    14701 ctccaaccgg ctccacagtc aagtctatcc cgctcatgac cggaacgcct gttaaaccgg
    14761 tatctcaggg taaagagtgg cgtgcgccgg caggcaccac attaagagag aacattatca
    14821 agtgggcaga agaaacaaaa tgcgaaagca tggcatcaac gcactggatg gtcatatggc
    14881 cactttctgt aaccgattac cgtctggatg cgcctttaat gttccgggga tcatttgaat
    14941 cagccatgga gcaggtattt gagttatatc ggactgcgca aaaaccttta tacgcacagg
    15001 cttctcgcat gcagtgcatc attacagttt ctgatacaag ggatggtcgc taatgggatg
    15061 gctggtcatg gccgtcggcc tgaccattct gattatcact ggtagctacc agaatcagaa
    15121 gatgtctgaa accacaaacg cccagcaata tgcaagtgcg tctgtatggg caagtcagat
    15181 actgatgatt gctaacagaa tcaatgatat cagatacgta tcaggccagc aggacggagt
    15241 aatttcctca gataaactgg ctctccctgt cactccggac tcccgaatta aacatcagtt
    15301 acaacagggg cgtctgtggg tatggatgcc tgaacaacca ggtcttgttg aaactttgcg
    15361 ttcaaaaagt cgtggttctg cactaatagg gatcttccaa aacgggcaac tcacctggct
    15421 atcaggaact gcaaccggtc tgacaccccc ggcaggcatt actgctggtt ctgtcgtcta
    15481 cgttaactaa tctggatata ttcatgaaaa aatcacacca gcgttcaatg aagctggcgg
    15541 tgctcccctg catgatcgcc gtcgctcttt ccatctccgg atgtactttc agcgaaatca
    15601 acaaaatgca gaaaaaagca caggaagact cagcacatgc acgggaaaag gtatcagccc
    15661 tttcggcccg taaatcgcag gctcttacct ggctcgataa tcaatggata aaccctgttc
    15721 cggtcgctca ggtatcaaga gagaaaaaac aaacagctcc ggcctgctac atcacgcagg
    15781 caagaaaagg agagatcact ctgcaggaac tggggcaacg tattactgcc gtatgcggca
    15841 tccctgtgat catcacgcct gacgcagcca attcaactct tgaaggaggc gctacccgcc
    15901 agatgacagg aacactacca gcaccagatg aaaatgggcg tctaccgtta agcagcctgg
    15961 gcagcacaac aatgactacc tccactcagc cattaacgct gaataacctc atgtggcagg
    16021 gagatatcaa tggtcttctg gatctgatgg ccagccggac tggtctgtac tggcgcatgg
    16081 ataatggtcg gattgtattc tatctgactg aaaccagaac gtatccactt catatgctga
    16141 acaccaaaac cagcagcagt tccagtgtca gctctggctc aacaagcaca atgggggcaa
    16201 caggaggcca ggataactca gcatccggtg atgcaacgtc ctctcagagc acaaccgttg
    16261 gtcaggaata cgatctgtat gaagacatcc ggaaaactat tgaagcaatg ctgacaccag
    16321 aaaaaggccg ttactggtta tctgcatcga gctcaacgct gactgtcact gatactccag
    16381 ctgtccagga agccgtcgca cgatatgtgg acgaacaaaa cagtattatg aaccgccagg
    16441 tagccctgaa cgtacaggtt ctgagcgtca gcaataccag aaacgaacag ttcggtctgg
    16501 actggaacct tgtttataaa tcgctacatt ccgccggagc aacgttgaac aatgcaagcg
    16561 gagattttac aggcgctaca tctgcaggcg tatcaattct ggatacggca acagggaatg
    16621 ccgccaaatt cagcggttcc agtcttctga ttaaagcgct gagtgaacag ggcgatgtca
    16681 gtgttgtgac ttcacaagaa agcactgtca caaacctgac gccggtacct atccagatgg
    16741 cagatcagac ggtttacgtc gcccagtcag caacaacaac gactacggat gtaggagcaa
    16801 caacaacatt aacgccgggc atgatcacca ccggattcaa tatgaccctg ctgcctttaa
    16861 ttcagaaaac gggcaatctc cagttgcaga tgaattttaa tctgtcagat cccccaacaa
    16921 tccgtagctt tacgtcaaaa gacggaaaca gttacatcga aatgccgtat accaaactgc
    16981 gttcactgag ccagaaggtc aatctgaaag aagggcaatc acttgtcgtt actggtttcg
    17041 atcagaacaa tacgacgaca agtaaagccg gtacgtttac gccagcaaat ccattatttg
    17101 gtggttcaca aaccgggaaa aatgaacgca gcacgcttgt aatcatcatt accccgactt
    17161 tcccgtcagg aggcaacaat ggctgatgaa gatatcaacc cggttgtact tctcgcagat
    17221 cctaaagtaa atcatcgcgt gtgggcagcc tgtcttaaat ggagtcctgt cgtaaaaaaa
    17281 cagcgagtcc cttctcatca gaaacacaag ccccatgtaa aatccagacg gcttacctct
    17341 ctgaaagtga ctgtaggcag cagaagttcc cgtgggaaga tcagccgcat taccggcaca
    17401 ggaatactgg ccaggccgga gcgcaatcat tatttttccc ttgctctggc tttctgctca
    17461 tgggtgagaa atggatacgg cgtcttccgg tacagcgata aggagctttt attccttgcc
    17521 tccattaatg gccagcctgc cgttatggct gatctgtcag gaaatgatgc tgatgttgct
    17581 caaaaggtca gtctttttct gacgatgaac gaagagcccc ccgaaaaatg gcaggtcgta
    17641 tctcctctgg aacatcctga taactgggaa agtattatta cccggttatc atcagcagat
    17701 ctccgccgtt gcaaacttac tgtcggcaat cgcagcaaat tcacactgcc tgccgtactt
    17761 tttttagtag cagccagcgc aggcactgta ttctggatga ctcagccaga gccggatgtc
    17821 gtaccgacgc ccgaggagat agctgcacgg gcaagattgc agtttaaaaa gcccgatcca
    17881 ccgccagaac tcccgcatcc atgggcatca caacctgtaa tcagtgattt tctgaaagcc
    17941 tgcgcggacc ttcgaaaacc atcaccggtc gcgctagaag gctggaaact taccggtgga
    18001 acatgtactc ccgagacatt cacactgatt tacgaacgac agccaggagg aactattgaa
    18061 ggctttctgg caagaagcaa agaaatcttt aatgttattc ctgactttaa ccttaaagat
    18121 ggtgcacgcc tggcttctgt aacccggcca ctgccttccc tgccccgcag ggatgaggca
    18181 gtccctaccc cctcagaaca actgatgcgg gtgttcacgt ggtttcagaa aaaacagtta
    18241 acgccggcca ttaatgaaat agccataccg gaaccattac caggtaacga tggtgaacca
    18301 gcccctgttc agaaatggaa agaatatcag ttcagtttat caacgcctgt aaatcctgat
    18361 gagttgttcc ctttatttca ggataccggc gttcgtctga gcaatatcca ttttgaatta
    18421 aacggcggta ctttcagtta ttcctcagaa ggtcatattt atgcgtccag gtaaattact
    18481 cattattcca tccatatttt tcttctccgg tttttcattt gcaacaacac aacctcttgt
    18541 cacaatagga gaacttgaag cacaacaaaa ccggaatatt cttttacagg caaaagttca
    18601 gggcgcacag ctacagaaac aactcgaaga atcagatgtt acttcatcat ctgaaactgt
    18661 ttcaggtttc tctggtgtga ctgcctcttt gccatcagtt tcagagcagc cgacatctaa
    18721 aaacagaaag gaattgcccg ttattatgga aataaacggc aaagataaac gtcttaatgc
    18781 cgttcttcgc atggctgatg gacgtcagac aagcgtcacc actggcagcc agcttccagg
    18841 cacatccgtc acagtgaagt cgatctcatt gtctggcgtc acgttaagtg atggcaccac
    18901 ccttactttc tgagtaacgt gatatggata aatataatct gaaggatgct ctccttttta
    18961 tatccagggg agacacccat gaaattctta tagaaacaaa ccagcggaca aggcctgacg
    19021 tacaaagtaa tctccaggag ctgctaaaat tatatccgga tatcaaccca aaagtagtct
    19081 cgttgtcgga gttacaggag gcaggtcagg atgagaaaaa cagaggcccg aaagaacgaa
    19141 ttattcatct gaaagacctt gctgatgtca gtgaaagtga gaagaaagtt ctatcttatt
    19201 ttgaaactgc cagaaaactt ggtgcttcag atattcattt cttaatttca gagtctattt
    19261 tcaaagttcg catgagaata ttcggtgaac tgcagactgt agatgaagat caaccagcac
    19321 tgggctattc actttgcgca acagccattc tgtcaatggc tgatgtcact gaaaccagtt
    19381 tcttccctca aagggagcag gatgcgcgcc tgtccccaca attaatgcga aaaataggaa
    19441 tatttggtgc acgctacagt cacagaccta cgggagacgg tctgattgca gttatgcgcc
    19501 tgatacccga tgacggagat aaagttccca cgtttaaaca actgggcttt atcccggaac
    19561 aaatccggtt gctgaacata atgctgcgcc gcccggaagg aaaaatagtc ctgtcaggtc
    19621 caaccggttc cggtaaatcg acaacgttac gcagcgcctg ccgtgtatat cttgacgata
    19681 atcaggggcg acacctgcta acaatcgaag atccgctgga aggacagata ctgggagcaa
    19741 cacagactcc gattatctgc gacaaatccg acgaagatgc cgtcaaactg gcatggagtc
    19801 gggctatttc atcggcaatg cgacttgacc ctgatgccat catggaaggg gaaatgcgcg
    19861 atcttatctc gatgatgtca accacctacg cggcacagac cgggcacata gttctgacga
    19921 cattacatac aaactcagct ctgggtatac cggagcgaat gattacgatg ggtatgaatg
    19981 ccgatcttat ttgcgatgcg caattactga ttgggatgat cagccagcgc cttgtaccaa
    20041 ctctgtgtcc ctcctgccgt attccatggg aaaagcgagc acctgaactt agcgacgacg
    20101 agcgtgatta ccttgagcgg cactgcaata aagattcact ctgcagtaca gataatatct
    20161 ggttccgaaa tcatcacgga tgctctgaat gtaatcacga cgtaatcatt aacggcagaa
    20221 agcgcggtga aataggtaaa ggcctcaccg gcaggactgt aattgctgag gtgatagaac
    20281 ctgataaccg cttatttcag attctgaaaa cacgcggcaa agttgcagca cgtaaatact
    20341 ggctggaaaa catgaaaggc atcagtcgtg tagagcatct tcttcgccgt atcaatgaag
    20401 gtctggtaga ccctcttgaa gccgacagaa tcatccctct ggacgaagac gagcgcctca
    20461 gcatcgatga tgtctaacag gagaacaaca tgcgtgaaat gaatttcagt caacggctga
    20521 ggcgtttcat tgtcagaaaa acgttcagcg ccccctacag ggtgcagttt tacgaagccc
    20581 tgcgttttct gctggaaaat aaacagccat taaagaccgc actggaacag atgcgggatg
    20641 cctggacaga ctttggacga aaatggcatc cctttgctga actggccaca gactgcattg
    20701 agtctcttcg tgaaaacagt ggcgaaaact ctctggaata tactcttagt ttatgggttc
    20761 ctcaggaaga ggccgcagta atcagcgccg ggataagaag tggttcaatt gtggatgcac
    20821 tgcaattcgc caccactctt acagatgcaa aagaacaaat ccatcaggct atatggcaaa
    20881 tggctattta tccggtggga ttgttaatca tgatgacggg gacattatat gttctgaata
    20941 cagagctaat acctgaactg agcaaaataa gctcccccga ttcatggagt ggtgctcttg
    21001 gttttcttta tggattatct gtttttgtcg ataattacgg cgctatatgt gccgttcttt
    21061 ttgcagtgat cacaggttta atatcatggt ctctgccaaa ctggaaaagc cctgacagtg
    21121 ttcgtacttt tgccgacaag ataatgccat ggagtattta tcaggatata cagggagcaa
    21181 catttctcct gaatatggct gcacttctga aagccaaaat gacgacgctc aactcgctaa
    21241 acattcttca ggagtttgca tcaccatggt taagcacacg tcttgacagc attatttacc
    21301 gcgtccgcca gggggatcat cttggactgg ctttgcgtca gtgcggttat cagttcccat
    21361 ccagggaagc agccaacttc ctgtctttat tacagggaga tggcgcaaca gagctaatca
    21421 gcaattatgg tcaacgatgg cttagtcaaa cacttcaacg tgtaaagaaa cgggcaaacg
    21481 tgattcgttt aattatgctg atatttcttg tcatgtctct catgttactg gtttttgcca
    21541 ttatggatat tcagtctatt agtgacaaca gcatgggtaa cttttaaaca aaacgaggat
    21601 atttatgtta gtagaaaata ttaataccac tttaactgga aataataaga agaatgagcc
    21661 tcatgataaa ggatgggcaa ttttggagca gggaacaata gcgctcgttg ttctttttgt
    21721 tatagtcgtt gttctgggta gtttgtatgc gttgcgtaca agagccaatg tggcaactga
    21781 aaccgcaaat atacaaacaa ttatcacaag tgcccagagc ctgctaaaag gtagcgatgg
    21841 atatacattt accagcagtg ccaaaatgac aggtgccctc attcagatgg gtgtcattcc
    21901 ctctgggatg accgtacagg gcgacaaaac atctggtaca gcaacgctct ataacgcatg
    21961 gggcggtgct gtaactgttg ccccggcttc aacatcaggg ttcaacaacg gttttaccgt
    22021 tacctatgat aaagtcccac aggatgcctg tatccagatt gccacacgaa tcagtaaaac
    22081 tggtttaact aacggcatta ccctgaacag tactgctcac agtgatggaa aagtcactac
    22141 tgaagaagcc agtacgcaat gcaaggcaga taatggcagc actggtacaa acaaacttat
    22201 cttcaccatc aatggttaag gagttttaat gtcagggggt acagtacccc tcagacttat
    22261 ttcattaatc ctgctttttt ctcttattcc acccgcccga gcttcatacg tagctcccaa
    22321 acagatctgg accaacaagt gggacaattg ctttgccgct gccggcgcgc gctatcaaat
    22381 tgaaccatta ttattgaagg ctatttctgc cggggaatct tcgttgaggc cgggcgcaat
    22441 taatataaat aaggacaaaa aaacaggtaa agccagtagc acggattatg ggttgatgca
    22501 aataaactca acccatattc caaagctgat aaatatgggc gtaataaaaa agtctgagga
    22561 tttaattacc aaaccatgcc ttaatatcca tataggaagc tggatactgg caagacattt
    22621 tcagatatgt ggcgtcagct ggaattgcct tgggtcatac aatgccggat tccgtaagga
    22681 cagacatgaa acaagagagc aatatgccaa taaaatatgg agaatttatc gtgacatgaa
    22741 agggatatgt cttcccggac agggaggcag gcaatgcaga caatcgtagg cataagtcta
    22801 tcccctgttt atatactgcc attgatgttc acattttcca ttattggacg aactctgcta
    22861 ttcagagttc gttatttttt atctgatacg ggatatctct ggtataagac acaccctgct
    22921 gttctgtcag gaatatggct atacagtata gctgtggcgt taattatttt atcatcatca
    22981 ccactgctct accgtattca tgctgtcctt attctttcgt tcatactgca aatggcggta
    23041 acggatgcac tgacaggttt attaccagga acattcaccc gacgtttttt aattgcagga
    23101 atgctatcac aaatcacgac tgatatatgg tggttccgta caacagaatt tgccactgct
    23161 gcaattgtct tattctgcct tcataaatta gttaaccgcc atcgtcttaa tattggcact
    23221 ggagacctgt ggcttattgc aggtattacc gcatggtcag ggttatataa cgccatatgg
    23281 tgtgtgttgc tgggcacagg aggatttgtc ttatggcatt caacatggtg cataaaaggg
    23341 cataaagaag gccctctggg tccatggcta tgttttggcc atgtcctttt gctgctggat
    23401 aatctttatc aaccactatg ggtaatctga tgaaaaaata tgaccgaggc tgggcatcgc
    23461 tggaaactgg cgcggcttta ctgattgtta tgctcctgat tgcctgggga gcgggcatat
    23521 ggcaggacta tattcaaaca aaaggatggc aaactgaagc acgtcttgtc agcaactgga
    23581 ccagtgcggc ccgctcttat atagggaaaa actacacgac tctccagggc agtagtacca
    23641 caacaactcc tgccgttatc acgacaacca tgctgaaaaa taccggcttt ttgtccagcg
    23701 ggtttactga gacaaacagc gaggggcagc ggttacaggc atatgtggtt cgaaacgccc
    23761 aaaacccgga attactacag gcaatggttg tatccagtgg tggcacacct tatccagtga
    23821 aagcacttat ccagatggct aaggatatta ccactggtct tggtggatat atccaggacg
    23881 gcaaaacagc cacaggtgca ttacgttcct ggtcagtagc tttaagtaat tatggtgcca
    23941 aaagcggtaa cgggcatatt gccgtattgt tatcgacaga tgaacttagt ggtgcagctg
    24001 aggacactga tcgtctttac agattccagg tcaatggtcg ccctgactta aacaaaatgc
    24061 acacggccat tgatatggga tcaaataacc tgaataacgt tggggcagta aatgcccaga
    24121 caggtaattt cagcggcaat gtgaatggtg taaatggcac tttcagcggt caggtaaaag
    24181 gcaatagcgg aaactttgac gtaaatgtca ccgctggcgg tgatatcaga agtaataatg
    24241 gttggttaat tactcgtaac agtaaaggct ggctcaatga aactcacggt ggcggatttt
    24301 atatgtccga tggatcatgg gttcgaagtg taaacaacaa gggcatctat accggcggtc
    24361 aggtgaaagg cggtactgtt cgggctgatg gtcgccttta tactggtgaa tacttacaac
    24421 tggaaagaac tgccgttgct ggcgcatcat gttcgcctaa cggccttgta ggccgcgata
    24481 atacaggggc aat
//
LOCUS       DAESHI010000057        24429 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.057, whole genome shotgun sequence.
ACCESSION   DAESHI010000057 DAESHI010000000
VERSION     DAESHI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24429
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.057"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..226)
                     /locus_tag="KW011_002382"
     CDS             complement(<1..226)
                     /locus_tag="KW011_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660693.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNG"
     gene            complement(342..893)
                     /gene="maa"
                     /locus_tag="KW011_002383"
     CDS             complement(342..893)
                     /gene="maa"
                     /locus_tag="KW011_002383"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HBH7660694.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(1064..1282)
                     /gene="hha"
                     /locus_tag="KW011_002384"
     CDS             complement(1064..1282)
                     /gene="hha"
                     /locus_tag="KW011_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBH7660695.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(1308..1682)
                     /gene="tomB"
                     /locus_tag="KW011_002385"
     CDS             complement(1308..1682)
                     /gene="tomB"
                     /locus_tag="KW011_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HBH7660696.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(2228..5377)
                     /locus_tag="KW011_002386"
     CDS             complement(2228..5377)
                     /locus_tag="KW011_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HBH7660697.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(5400..6593)
                     /gene="acrA"
                     /locus_tag="KW011_002387"
     CDS             complement(5400..6593)
                     /gene="acrA"
                     /locus_tag="KW011_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HBH7660698.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            6735..7382
                     /gene="acrR"
                     /locus_tag="KW011_002388"
     CDS             6735..7382
                     /gene="acrR"
                     /locus_tag="KW011_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HBH7660699.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            7510..10872
                     /gene="mscK"
                     /locus_tag="KW011_002389"
     CDS             7510..10872
                     /gene="mscK"
                     /locus_tag="KW011_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HBH7660700.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(11084..11245)
                     /gene="rsmS"
                     /locus_tag="KW011_002390"
     CDS             complement(11084..11245)
                     /gene="rsmS"
                     /locus_tag="KW011_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HBH7660701.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(11259..11786)
                     /gene="priC"
                     /locus_tag="KW011_002391"
     CDS             complement(11259..11786)
                     /gene="priC"
                     /locus_tag="KW011_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HBH7660702.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            11856..12233
                     /locus_tag="KW011_002392"
     CDS             11856..12233
                     /locus_tag="KW011_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HBH7660703.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            12386..12937
                     /gene="apt"
                     /locus_tag="KW011_002393"
     CDS             12386..12937
                     /gene="apt"
                     /locus_tag="KW011_002393"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBH7660704.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            13066..14991
                     /gene="dnaX"
                     /locus_tag="KW011_002394"
     CDS             13066..14991
                     /gene="dnaX"
                     /locus_tag="KW011_002394"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBH7660705.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    14324..14388
                     /gene="dnaX"
                     /locus_tag="KW011_002394"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            15044..15373
                     /locus_tag="KW011_002395"
     CDS             15044..15373
                     /locus_tag="KW011_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBH7660706.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            15373..15978
                     /gene="recR"
                     /locus_tag="KW011_002396"
     CDS             15373..15978
                     /gene="recR"
                     /locus_tag="KW011_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBH7660707.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            16088..17962
                     /gene="htpG"
                     /locus_tag="KW011_002397"
     CDS             16088..17962
                     /gene="htpG"
                     /locus_tag="KW011_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBH7660708.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            18143..18787
                     /gene="adk"
                     /locus_tag="KW011_002398"
     CDS             18143..18787
                     /gene="adk"
                     /locus_tag="KW011_002398"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBH7660709.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            18919..19881
                     /gene="hemH"
                     /locus_tag="KW011_002399"
     CDS             18919..19881
                     /gene="hemH"
                     /locus_tag="KW011_002399"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HBH7660710.1"
                     /translation="MRQTKIGILLANLGTPDTPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(19878..20837)
                     /gene="aes"
                     /locus_tag="KW011_002400"
     CDS             complement(19878..20837)
                     /gene="aes"
                     /locus_tag="KW011_002400"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HBH7660711.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            20989..22293
                     /gene="gsk"
                     /locus_tag="KW011_002401"
     CDS             20989..22293
                     /gene="gsk"
                     /locus_tag="KW011_002401"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HBH7660712.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(22426..24102)
                     /locus_tag="KW011_002402"
     CDS             complement(22426..24102)
                     /locus_tag="KW011_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HBH7660713.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
BASE COUNT         6056 a         6367 c         6301 g         5705 t
ORIGIN      
        1 aaccattaag caggataaat aatacaacca gtagccagac agagcctgac agcgtttcgg
       61 actgcaacat caccgccagg gtggcaaaaa aagcaacgta cataccgata aacagccccg
      121 gatgtttacg gataaaactg atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat
      181 tcagagactc aatcgtttcg gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg
      241 gtatctgcaa ctttccaggg aacaccctat tttaacgtgg gaatagtaga ggggaagtaa
      301 cagatttacc acaattttgc ataacagttg cgaaaaaccg attacaattt tttaattatt
      361 ctggctggat taccgcccac gacaacgttg tccgggacat cttttgtgac aactgcacct
      421 gaggctacca cgacgttatc accaatggtc acaccagggt taatgaccgc gcgtccgcca
      481 atccagacgt tattaccgat ggtgacgggt ttccccagtt cagcaccgct attacgtgct
      541 acagggtcga tgggatgtgt tgccgtgtag atatgaacgc ctggtgccaa catacagtta
      601 tcaccgatgc gaatagggca gacatcaagc atcacgcaat cgaagttggc gaaaaaatta
      661 ttaccgagaa aaatgttata gccatagtca cagcgaaacg ttggctcaat ataagcctct
      721 gtcacctgac cgaatagatc agcgagaatt tgctggcgta atgtgtgctc ttccgccagg
      781 gaatgattgt atcggtgaat aagctgacga gcgcgcaggc gatcgcgaga taacgtctca
      841 tctgccgagc gatacaactc accagcaatc atcttttctt tttctgtgct cattactgac
      901 ctccggttgc tgaaaatgcg gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc
      961 cattacatct gtgtgataaa gatcacatag ggtatgtctt catggtgaaa agtataaaat
     1021 tcttaataaa cagccggtta tagctccgaa agcgaattat ttattagcga ataaatttcc
     1081 atactgagga agggatcttg tcgtatagtt tattcatggt caattcggcg aggcggtgat
     1141 ctgcggctga gtaaaatacc gccagttcat tatctgataa ttcgtattta tttttctcga
     1201 taacacgctc cagcgtgtca attgtctggc aacgacgtaa acgcattaaa taatcggttt
     1261 tcgttaaagg tttttcggac atacttctac ccatggttgt aataattcta acaagataaa
     1321 ctcgcaggat tctctttcgt cgcattgaca aaacaacgga atagtcgatt acctgacttc
     1381 cgccatttct gaagatcctg catattaata ccataactac tgaacaacat aaaggtgtca
     1441 tccagatatt cgtcgatctg ctcaatgagc ttattgtctt cattatactt aattttgtaa
     1501 ttaagtgcga aggtcgcaat atgctcaatc agttcattca actggaggtt gatcgccgag
     1561 gttgggtcgt taacccagcc atgattgctt tcttcaaggt ttgcaaggca gtcatgatac
     1621 agggtttcac agagaaactt aagctgtgcg atatcatgtc ttttgggtga gtattcatcc
     1681 ataacgcgtc cccttcttag cggttgaact aacggacacc tttcgggatg gaaaaaactt
     1741 actgacctgg acttgccctc tcgtttgtta gcttaactat aagccactct ttgcaggttt
     1801 tcatcgcatt attacgaaaa attacaatta ggaaggaatc gggacaggac tcactttttc
     1861 atactagcac taacccgcag caggtaaaag cagtctgctc gaactgttca taacatttca
     1921 ggattatcgc cagatgaaat tggtgacact aataccagga ttgctctgaa tatgacgtaa
     1981 taaccgagga atgaataaag aattaccgca aataattaag aatagcctct aaatgattat
     2041 ggattattaa atctattagc gctacttaat ataaattaac taacaatcag tattttttat
     2101 gaattttatc cgtggttaat actggttttc gtatgagatc ctgagttggt ggttcaatta
     2161 ctccttaatg ttcgtaggtt atgcataaaa aaggccgctt gcgcggcctt agtgattaca
     2221 cgttgtatca atgatgatcg acagtatggc tgtgctcgat atcttcattc ttgcggctaa
     2281 agcggcggcg aaccaccaca aagaataccg gaacgaagaa gattgccagt accgttgcgg
     2341 tcaccatccc gcccattaca ccggtaccta ctgcgttctg cgcgccggaa ccagcaccag
     2401 tactgataac cagcggcata acgccgagga taaacgccag cgaggtcatc aggatcggac
     2461 gtaaacgcat ccgcactgca tcaagcgtcg cttcaatcag acctttacct tctttatcca
     2521 tcaagtcttt ggcgaattcg acgataagta tcgcgttctt cgccgacaac ccaatggttg
     2581 tgagcaggcc tacctggaag taaacgtcat tggtcaggcc acggaaggtg gcagccagca
     2641 acgcaccgat aacccccagc ggaacgacca gcataacgga gaacggaatc gaccagctct
     2701 cgtacagcgc cgccagacac aggaatacga caatcaacga aatcgcgtac agtgaaggtg
     2761 cctggttgcc ggagagacgt tcctgatagg acatccccgt ccagtcatag ccaacaccgg
     2821 taggcagttt gctcgccagt tgttccatca gctccattgc ttcaccggta cttttacccg
     2881 gtgccgcctg gcctaagatt tccatggatg gcaggccgtt gtaacgttcc agacgcggcg
     2941 aaccgtactc ccaacgagaa gaggagaacg ccgagaatgg caccatctga ccatcagcag
     3001 cacgaacata ccagtcgccg atatcatccg gcagcatacg gtatttcgct tctgacatga
     3061 cgtaaacttt cttcacacga ccgcggtcga taaagtcgtt cacatagcta ccgccccatg
     3121 cagcgcccag agaggtgtta atgtcgttga tagaaacacc cagcgcctgc gctttttcct
     3181 ggtcgatatc aatcttaaac tgcggggtat cttccagacc gtttggacgt acgctggtca
     3241 gcatatcagg gtgcttcgct gcttctgcaa gcagctggtt acgcgcctga gtcagttttt
     3301 cgtgaccaag gccagcctgg tcaatcagct caaagtcaaa gccggttgca gtacccagtt
     3361 ccacgattgc gggcaggtta aaggcgaaaa ccatcgcatc tttgatttgc gagaaagcgc
     3421 gtgttgcacg catggtaatc gcttcaactt tgttttcttc gcccggacga tcggcccagt
     3481 ccttcaagga aacgaacgca ataccggtat tctgaccacg tcccgcaaag ccgaagccgt
     3541 taacggcgaa caccgactca acgttgttct tttctttggt cagatagtaa tgcgttacct
     3601 cattgagcac tttctgcgta cgttcctgcg ttgcacctgc tggcagctga accatggtca
     3661 taaatacgcc ctggtcctca tctggcaaga aggagcttgg cagacgcacg aacagatagg
     3721 ccatgccgac cacgatgatc agatacagca ccaggtaacg ccccgtactg cgcagaatac
     3781 cgcctacgct gtcggtgtag tggtgcgtgc tcttctcgaa catgcggtta aaccagccga
     3841 agaagccttt tttaccttcc ccgtgatcgc ctttggcaat cggtttcagc atggtggcac
     3901 aaagagccgg agtcaggatc aacgctacca gtaccgacag cgccattgct gaaacaatgg
     3961 taatagagaa ctgacgatag atagcaccag tagaaccgcc aaagaaggcc atcggtacga
     4021 ataccgccga cagtaccatc gcgataccga ccagagcgcc ctgaatctgc cccatcgact
     4081 tacgggtagc ttcttttggc ggcaaacctt cttccgccat aacacgctcc acgttttcta
     4141 ccacaacgat agcgtcatcc accaacaagc cgatggcgag caccatcccg aacattgtta
     4201 gcgtgtttat cgagaagcca aaggcggcaa ggacggcaaa ggtcccgagc aataccaccg
     4261 gtacggcaat ggtcggaatc aacgtcgcgc ggaagttctg caggaacaga tacataacca
     4321 ggaacacgag gatgatcgct tcgaccagcg ttttaaccac ttcgtgaata gagattttca
     4381 cgaacggcgt ggtgtcgtac gggtaaacaa ttttcagacc cgacgggaag aacggttcca
     4441 tcttcgccag ttcagcacgg attgccgcag cggtatccag cgcgtttgca ccggtcgcca
     4501 gcttgatccc cagaccggaa gccggttggc cgttaaactc tgcgatgatg tcgtagttct
     4561 caccacccag ctcaatcttc gccacatcac gcagcagtac gcgggaacca tcctgattca
     4621 ctttcagcag gattttgccg aactcttcag tagaggtcag acgcgtctga gcaataatag
     4681 aggcgttaag ctgttggcct ttcaccggcg gcgtaccacc gagctgaccc gccgcaacct
     4741 gggcgttctg cgctttgatg gcggtaatga catcaaccgg cgttagctgg aatttgttca
     4801 gctcattcgg gttcatccag atacgcatcg cgtactgtga accgaacaac tgaacatcac
     4861 ccacgcccga cgtacggctg atggcatctt tcatattcgc cgctacgtag tcagagatat
     4921 cctcctgcgt catggtgcca tcggtgttga taacgccgac aaccatcagg aagctgctgg
     4981 atgatttctc aacgctcacc ccttgctgct gaacttcttg cggcagcaac ggcatcgcca
     5041 gctgcagttt gttctgcacc tgaacctgcg cgatatccgc atcagtacca gactcaaagg
     5101 tcagggtgat ctgcacggta cccgtggagt cactgttaga ggacatgtac atcaggttat
     5161 cgataccgtt catattctgt tcgataacct gtgtcaccgt gtcctgcact gttttcgcat
     5221 cagcgccggg gtaggaggcg gagatcgtta ctgccggcgg tgcaatcgta ggatattgcg
     5281 ccaccggcag tttgaggatc gccagccccc ctgccaacat gatgataatg gcgatcaccc
     5341 acgcaaaaat cgggcgatcg ataaagaaat taggcatgtc ttaacggctc ctgtttaagt
     5401 taagacttgg actgttcagg ctgagcaccg cttgcggctt gctggttatt atcagcggta
     5461 acttcttgtg cttttacctg gacaccagga cgcactttct gcagcccact tattactacg
     5521 cgatcgcctg ctttcagacc ttctgtcacc agccacttat cgccaatagc ctggcttgca
     5581 acgatcggac gggtttccac tttgtcatcc gcgccaacta ccagtacggt ggcatcacca
     5641 cgcggcgtac gggttacgcc ctgttgcggg actaaaatag cgtttggatt aagcccttct
     5701 tccagacgtg cgcgcacgaa catacccggc agcagagtgt gatccgggtt cgggaagata
     5761 gcgcgtaggg tgatagaccc agtggtctga tcaacggtaa cgtcagagaa ttccagcgta
     5821 ccgtcctgcg ggaacttaat gccgtcactg gtgatcagcg acactttggc tttgccgttc
     5881 tcttgtttca gcgtgccatt cgccagttcc tgtttcaggc gcaggaagtc gttgctggac
     5941 tgggtcacat caaggtagat cggatcaagt tgctgcacgg ttgccagcgc agtcgcctga
     6001 ccgttctgta ccaatgcgcc ttccgtcacg ttcgacttac caatgcgacc gctaatcgga
     6061 gaggtgactt tggtgtaagc cagattgatc cgcgcagttt caacggcagc tttcgccgca
     6121 gttaccgcag cattcgcctg ttgcgcatca gccagagcct gatcgtactc ttgcttactg
     6181 atgtactgag taccgagcag tttctgataa cgattcaccg tcaattgcgc gatattggct
     6241 gcagcctggg ctttcgccag atcacctttc gcactgtcgt atgtcgcctg ataggtcgca
     6301 ggatcaatct gatagagaga gacaccagct tcgatgtcgc taccttcttt gaaattacgc
     6361 ttcaggataa tcccgctaac ttgaggacga acttctgcga tccggtaggc actggtgcga
     6421 cccggaagct cggttgtgat ctgcagaggt tcagttttga ctgttactac gccaacggcg
     6481 ggcatctgct ggccaccttg ttgggcctgt ttgtcgtcac atcctgttag ggctaagctg
     6541 cctgagagca tcagaacgac cgccagaggc gtaaaccctc tgtttttgtt catatgtaaa
     6601 cctcgagtgt ccgatttcaa attggtcaat ggtcaaaagt taataaaccc attgctgcgt
     6661 ttatattatc gtcgtgctat ggtacataca ttcacaaatg tatgtaaatc taacgcctgt
     6721 aaattcacga acatatggca cgaaaaacca aacaagaagc gcaagaaacg cgccaacaca
     6781 tcctcgatgt ggctctacgt cttttctcac agcagggggt atcatccacc tcgctgggcg
     6841 agattgcaaa agcagctggc gttacgcgcg gtgcaatcta ctggcatttt aaagacaagt
     6901 cggatttgtt cagtgagatc tgggaactgt cagaatccaa tattggtgaa ctagagcttg
     6961 agtatcaggc aaaattccct ggcgatccac tctcagtatt aagagagata ttaattcatg
     7021 ttcttgaatc cacggtgaca gaagaacgac gtcgattatt gatggagatt atattccaca
     7081 aatgcgaatt tgtcggagaa atggctgttg tgcaacaggc acaacgtaat ctctgtctgg
     7141 aaagttatga ccgtatagaa caaacgttaa aacattgtat tgaagcgaaa atgttgcctg
     7201 cggatttaat gacgcgtcgg gcagcaatta ttatgcgcgg ctatatttcc ggcctgatgg
     7261 aaaactggct ctttgccccg caatcttttg atcttaaaaa agaagcccgc gattacgtcg
     7321 ccatcttact ggagatgtat ctcctgtgcc ccacgcttcg taatcctgcc actaacgaat
     7381 aaccctgaat ctgactccag gatttttcct ggacattttc gtcgttgcta ttctggttca
     7441 ctgcgtcgtg atattcttgc ggtttgactt tttcaggtcg ttcttcaggt tcagaaacct
     7501 tcattcatca tgactatgtt ccagtattac aaacgatcac ggcattttgt tttttcagca
     7561 tttattgctt ttgtttttgt cttgttatgc cagaacacgg cgtttgcgcg ggcgtcatcg
     7621 aatggtgatc tgccgacaaa agcggacctg caggcgcaac ttgactcact aaataaacaa
     7681 aaagatcttt ctgctcagga caaactggtg cagcaggatc tgacagatac attagccacc
     7741 ctcgataaaa tcgatcgcat aaaagaagag acagttcagc tacggcaaaa agtcgctgaa
     7801 gcgccggaaa aaatgcgcca ggcgaccgcg gcgttaacag cacttagcga tgtcgataac
     7861 gacgaagaaa cgcgcaaaat tctgagtacg ctttcgttgc gccagctgga aactcgcgtt
     7921 gcccaggcgc tggacgattt gcaaaacgca caaaacgatc tggcgtctta taacagccag
     7981 ctggtttcgt tacagacgca gcccgaacgc gtgcaaaatg cgatgtataa cgcttcgcag
     8041 cagctgcaac aaattcgcag tcgtctggat gggactgatg tcggcgagac tgccttacgt
     8101 cccagccaga aagtgttaat gcaggctcag caggcgttgc tgaatgcgga gattgaccag
     8161 cagcgtaaaa gcctggaagg gaacaccgtc ttgcaggata ccttgcaaaa gcaacgtgat
     8221 tacgtgacgg cgaacagcgc ccgtctggag caccagttgc agctgttgca agaagcggta
     8281 aacagcaagc gcctgacttt aactgaaaaa acggcgcagg aagccgtctc cccggatgaa
     8341 gccgcgcgta ttcaggctaa tccgctggtg aaacaggaac tggaaattaa ccagcagtta
     8401 agtcagcgtc tgattaccgc gactgaaaac ggtaatcagt tgatgcagca aaacattaaa
     8461 gtcaaaaact ggctggagcg ggcgctgcaa tcggaacgca atattaaaga gcagattgcc
     8521 gtcctgaagg gcagcctgct gttgtctcgt atcctttacc agcaacaaca aacgctgccc
     8581 tcggcggatg aactggaaaa catgaccaac cgcatcgcgg atttgcgtct cgaacagttt
     8641 gaagttaacc agcagcgtga tgcactcttc cagagcgatg cgttcgtcaa caaactggaa
     8701 gaaggtcaca ccaacgaagt caacagcgaa gttcacgatg cgttattgca agtggttgat
     8761 atgcgtcgcg aattgctgga tcaactcaac aaacagttgg gtaaccagct gatgatggcc
     8821 attaacctgc aaatcaacca gcagcagtta atgagcgtat cgaaaaacct gaaatccatc
     8881 ctgactcagc aaatcttttg ggtgaacagt aaccgtccaa tggactggga ctggatcaaa
     8941 gcgttcccgc aaagcctgaa agatgaattt aagtcgatga aaatcacggt gaactgggaa
     9001 aaagcctggc ctgccgtttt tatcgctttc ctcgccggtt tgccgctgct gttgattgcc
     9061 gggctgatcc actggcgtct gggctggctg aaagcgtatc aacagaaact ggcttccgct
     9121 gtgggttccc tgcgtaacga tagccagctc aacacaccaa aagcgatcct tatcgacctg
     9181 atccgcgcgc tgccggtgtg cctgattatt ctcgcggttg gcctgattct gttgaccatg
     9241 cagctcaaca tcagcgaact gctatggtcg ttcagtaaaa aactggcgat tttctggctg
     9301 gtgtttggcc tgtgctggaa ggtactggag aaaaatggcg ttgccgtgcg tcacttcggc
     9361 atgccggagc agcagaccag ccactggcgt cggcaaattg tccgcatcag tctcgcgttg
     9421 ctgcctatcc atttctggtc tgtggtggca gaactttccc cgctgcatct gatggatgat
     9481 gtgctggggc aggcgatgat tttcttcaac ctgctgctga ttgccttcct ggtatggcca
     9541 atgtgccgcg aaagctggcg tgataaagag tcgcacacca tgcgactggt caccattacc
     9601 gtgctgtcga ttatcccgat tgcattgatg gtgctgactg ccacgggtta tttctacacc
     9661 acgctgcgcc tctccgggcg ttggattgaa accgtttatc tggtgatcat ctggaacttg
     9721 ctctaccaga cggtattgcg cggcttaagc gtagcggcgc ggcgtatcgc ctggcgtcgt
     9781 gcgctggcgc gtcggcagaa tctggtgaaa gagggcgcag aaggtgccga accgccggaa
     9841 gaacccacca ttgcactgga acaggttaac cagcagacgc tgcgtattac catgttgctg
     9901 atgtttgcgc tgttcggtgt catgttctgg gcaatttggt ccgatttgat caccgtgttc
     9961 agctatctcg acagcatcac gctctggcat tacaacggca ctgaagctgg cgctgcggtg
    10021 gtgaaaaacg tcaccatggg cagtctgttg tttgcgatta tcgcctcaat ggtggcctgg
    10081 gcgttgattc gcaacctgcc tggtttactg gaagtgctgg tgctctcgcg actgaatatg
    10141 cgtcagggcg cgtcgtatgc cattactacc atccttaact acatcattat tgccgttggt
    10201 gcgatgacgg tgttcggatc gctgggcgtc tcttgggata aactccagtg gctggccgca
    10261 gcattatccg taggtcttgg ttttggttta caagaaattt tcggtaactt cgtctccggt
    10321 ttgatcattc tgttcgaacg tccggtgcgt attggcgata cggtaaccat tggtagcttc
    10381 tcggggacgg taagtaagat ccgtattcgt gcgacaacga ttaccgattt cgatcgcaaa
    10441 gaagtgatca tcccgaacaa agcgtttgtt accgagcgtc tgatcaactg gtcgttgact
    10501 gacactacta cgcgtctggt gatccgtctc ggcgtggcct atggctccga tctggaaaaa
    10561 gtgcgtaaag tgttactgaa ggcggcgact gagcacccaa gggtgatgca cgaaccaatg
    10621 ccggaagtct tctttacggc gtttggtgcc agcacgttgg atcatgagct gcgtctgtat
    10681 gtgcgtgaac tgcgtgaccg tagtcgtact gtcgatgagc tgaaccgtac tatcgatcag
    10741 ctgtgccgtg aaaacgacat caacattgcc tttaaccagc ttgaagtgca tctgcacaac
    10801 gagaagggcg atgaggtgac ggaagtcaaa cgcgactaca aaggcgatga cccgacgcca
    10861 gcggtagggt aataaccaaa gggggcgaca tttagttgcc ccgagattac tgacaaagtg
    10921 cgcgttgttc atgccggatg cggcgtgacc gccttatccg gcctacgaaa ccgcaagaat
    10981 tcaatgtatt gcaggagcgg tgtaggcctg ataagcgtag cgcatcaggc agttttgcgt
    11041 ttgcccacaa tcttagggga catttagcgg ccccatttat ttctcacttt tccgcctcat
    11101 catcgcgcgt taatttcttt tcataatcgc gttttacaat atccagcgcg cgcagcacgg
    11161 tgcgggctgg gatctgattt tcttccagca gcacaatcaa atcgacagcc agtttgacat
    11221 cgtcaggggc attttcaagt gacatactct ctccattgct agcgggttaa gcgcgctagc
    11281 ctgttttcga ttttttccag cgcatggcgg cagcgtgcca ggcgcgcttc ataggcttcc
    11341 acttcacgat gcagcgtttg ctgttccacg agatcggtca cccgcgccag gcgggctctg
    11401 cgttcggcaa ccatctcacg tagccgccgc tcaaaatcct gatgctgaat acgtttacgc
    11461 tgccagcggg caattttcgg tggcgcactg tcccactcgc gcaatgacca ggcggaggct
    11521 tcacgcgcga tggcttccag ttgtgccgcc agatgttccg ccagccaggc cacttgcggc
    11581 agctgttgct gctcaaccgc atgacaaagc gcagccagat tatcgcccgc ctcgtcgaga
    11641 caagcctgta gtgttgtcgc acgagtctga aaaagatgcc tgtcgaaacg agcacttagc
    11701 gtggcgaact gtgacaccgg ggcacaacgc tgacgcagcg tagcgagctg tccttccagt
    11761 ttttccagca gcagggcggt tttcacgata agacctcaat gaaaatgata attgttatgc
    11821 taaagtagcc actctgtaag ctgacaacac tcaatatgca acgaatcatt ttaatcatca
    11881 ttggctggct ggcggtagtg ctgggtacgc tgggcgtggt attaccggta ttaccgacga
    11941 cgccgtttat cctgctggcg gcctggtgct ttgcccgttc ttccccgcgc tttcacgcct
    12001 ggttgctgta ccgctcatgg tttggcagct atctacgttt ctggcagaaa catcatgcga
    12061 tgccgcgcgg cgtcaaaccg cgggcgattt tgcttatttt gctcacgttt gccatttctc
    12121 tgtggttcgt ccagatgcca tgggtgcgca tcatgttgct ggtaattctc gcctgtttgc
    12181 ttttctatat gtggcgaatt ccggtgattg atgaaaagca agaaaagcac tgaagcacaa
    12241 caatcgcagt tgcaattatt gcgtacagcc agtacattct ggcgttttcg agcacaggcg
    12301 caggcggtca aaggttaaac aactgttact tttgatacgt ttaaaacgcg ccgtgagtac
    12361 caccgtaaca agcaggcata cacttatgac cgcgactgca cagcagcttg agtatctcaa
    12421 aaatagcatc aaaagcattc aggactaccc aaaacccggc attcttttcc gcgatgtcac
    12481 cagcttactg gaagacccga aagcttacgc tctcagcatc gacttgctgg ttgagcgtta
    12541 caaaaatgcg ggcattacca aagttgtcgg caccgaagcg cgtggcttct tgtttggcgc
    12601 tccggtagct ctgggtctgg gcgttggctt tgtaccggtc cgtaaaccgg gcaaactgcc
    12661 gcgtgaaacc atcagtgaaa cttacgacct ggaatacggc accgatcagc tggagatcca
    12721 cgttgatgcc atcaaaccgg gcgacaaagt tctggtggtg gacgatctgc tggcaaccgg
    12781 cggcactatc gaagcgaccg ttaaactgat ccgtcgtctg ggtggtgaag tggctgacgc
    12841 tgcgttcatt atcaacctgt tcgatctcgg cggcgaacag cgtctcgaaa aacagggcat
    12901 taccagctac agccttgtcc cgttcccggg ccattaatta tcgccagtct tgtgctgccc
    12961 acgctacgga cagcacaaga tgtgcattca gcctcgccgt tctgacgggg ctgtgttagc
    13021 attacccctt cgtgaatcca ccttccagcg tttcagagcc tgccaatgag ttatcaggtc
    13081 ttagcccgaa aatggcgccc acaaaccttt gctgacgtcg tcggccagga acatgtgctg
    13141 accgcactgg cgaacggctt gtcgttaggg cgtattcatc atgcttatct tttttccggc
    13201 acccgtggcg tcggaaaaac ctctatcgcc cgactgctgg cgaaggggct aaactgcgaa
    13261 accggcatta ccgcgacgcc gtgcggcgtg tgcgataact gtcgtgaaat cgagcagggg
    13321 cgctttgtcg atctgattga aatcgacgcc gcctcgcgca ccaaagttga agatacccgc
    13381 gacctgctgg ataacgtcca gtacgctccg gcgcgtggtc gtttcaaagt ttatctgatc
    13441 gacgaagtgc atatgctgtc gcgccacagc tttaacgcac tgttaaaaac ccttgaagag
    13501 ccgccggagc acgttaagtt tctgctggcg acgaccgatc cacagaaatt gccggtgacg
    13561 attttgtcgc gctgtttgca atttcatctc aaggcgctgg atgtcgagca aattcgccat
    13621 caacttgagc acatcctcaa cgaagaacat atcgctcacg agccgcgggc gttgcaattg
    13681 ctggcgcgcg ccgctgaagg cagcctgcga gatgccttaa gtctgaccga ccaggcgatt
    13741 gccagcggtg acggccaggt ttcaacccag gcagtcagtg cgatgctggg tacgcttgat
    13801 gacgatcagg cgctgtcgct ggttgaagcg atggtcgagg ccaacggcga gcgcgtaatg
    13861 gcgctgatta atgaagccgc tgcccgtggt atcgagtggg aagcgttgct ggtggaaatg
    13921 ctcggcctgt tgcatcgtat tgcgatggta caactttcgc ctgctgcact tggcaacgac
    13981 atggccgcca ttgagctgcg gatgcgtgaa ctggcgcgca ccataccgcc gacggatatt
    14041 cagctttact atcagacgct gttgattggt cgcaaagaat taccgtatgc gccggaccgc
    14101 cgcatgggcg ttgagatgac gctgctgcgc gcgctggcgt tccatccgcg tatgccgctg
    14161 cctgagccag aagtgccacg ccagtccttt gcacccgtcg tgccaacggc agtaatgacg
    14221 ccaacccagg tgccgccgca atcagcgccg cagcaggcac cgactgtacc gctcccggaa
    14281 accaccagcc aggtgctggc ggcgcgccag cagttgcagt gcgtgcaggg agcaaccaaa
    14341 gcaaaaaaga gtgaatcggc agccgctacc cgcgcgcggc cggtgaataa cgctgcgctg
    14401 gaaagactgg cttcggtcac cgatcgcgtt caggcgcgtc cggtgccatc ggcgctggaa
    14461 aaagcgtcag ccaaaaaaga agcgtatcgc tggaaggcga ccactccggt gatgcagcaa
    14521 aaagaagtgg tcgccacgcc gaaggcgctg aaaaaagcgc tggaacatga aaaaacgccg
    14581 gaactggcgg cgaagctagc ggcagaagcc attgagcgcg acccgtgggc ggcacaggtg
    14641 agccaacttt cgctaccaaa actggtcgaa caggtggcgt taaatgcctg gaaagaggag
    14701 agcgacaacg cagtatgtct gcatttgcgc tcctctcagc ggcatttgaa caaccgcggt
    14761 gcacagcaaa aactggctga agcgttgagc atgttaaaag gttcaacggt tgaactgact
    14821 atcgttgaag atgataatcc cgcggtgcgt acgccgctgg agtggcgtca ggcgatatac
    14881 gaagaaaaac ttgcgcaggc gcgcgagtcc attattgcgg ataataatat tcagaccctg
    14941 cgtcggtttt tcgatgcgga gctggatgaa gaaagtatcc gccccatttg atcgtaagca
    15001 cagcttacgt tcgtcatcct taacgtgatt gagagagaaa cctatgtttg gtaaaggcgg
    15061 tctgggtaac ctgatgaagc aagcccagca gatgcaagaa aaaatgcaga aaatgcagga
    15121 agagatcgcg cagctggaag tcaccggcga atctggcgca ggtctggtaa aagtgaccat
    15181 caacggtgca cacaactgcc gtcgcgtaga gatcgacccg agcctgctgg aagacgacaa
    15241 agagatgctg gaagacctgg tggctgcagc attcaacgac gcagcacgtc gtattgaaga
    15301 aacgcagaaa gaaaaaatgg cctctgtatc ctccggaatg cagctgccgc ctggctttaa
    15361 gatgccgttc tgatgcaaac cagcccgctg ttaacacagc ttatggaagc actgcgctgt
    15421 ctgccgggcg ttggcccgaa gtcggcgcag cgtatggcgt tcacgctgct tcagcgcgat
    15481 cgtagcggcg ggatgcgttt ggcgcaggcg ctcacccggg cgatgtcgga aatcggccac
    15541 tgcgccgatt gccgcacttt caccgaacag gaagtctgta acatctgttc gaatccgcgt
    15601 cgtcaggaaa acggtcaaat ctgtgtggtg gagagtccgg cggacatcta cgccattgag
    15661 cagacggggc agttttctgg tcgttatttt gtgttgatgg ggcatctgtc accgctggac
    15721 ggcatcggtc cggatgatat cgggcttgat cgtctggaac agcgtctggc agaggaaaaa
    15781 atcactgaag tgatcctcgc caccaatccc acggttgaag gtgaagctac cgctaactac
    15841 attgccgagc tttgcgcgca atatgacgtg gaagccagcc gaatcgctca tggcgtaccg
    15901 gttggcggcg aactggaaat ggtcgatggc accacgctgt cacactccct tgccgggcgt
    15961 cataagattc gtttttaagc aaacgagagc aggatcacct gctctcgctt gaaattattc
    16021 tcccttgtcc ccatctctcc ctcatcctgt ttttaacctt aaaatggcat tattgaggta
    16081 gacctacatg aaaggacaag aaactcgtgg ttttcagtca gaagtgaaac agcttctgca
    16141 cctgatgatc cattctctct attccaataa agaaatcttc ctgcgtgagc ttatctctaa
    16201 cgcctccgat gcggcggaca agctgcgttt ccgtgcgcta tctaacccgg acctgtacga
    16261 aggtgatggc gaactgcgcg ttcgtgtctc tttcgataaa gacaagcgca cgctgaccat
    16321 ctctgataac ggcgtgggga tgacccgcga cgaagtgatt gaccatctgg ggactatcgc
    16381 taaatccggt accaaatcat tcctcgaatc cctgggttct gaccaggcga aagacagcca
    16441 gctgatcggt cagtttggtg ttggtttcta ctctgcgttt atcgtggccg acaaagtgac
    16501 cgtgcgtact cgcgcggcag gcgaaaaacc agaaaacggc gtcttctggg aatcggctgg
    16561 cgaaggtgaa tataccgttg ccgacatcac caaagaagat cgtggtactg aaatcaccct
    16621 gcatctgcgt gaaggcgaag acgagttcct cgatgactgg cgcgtgcgtt ccatcatcag
    16681 caaatactcc gaccatatcg cgctgccggt agagatcgaa aaacgcgaag agaaagacgg
    16741 cgaaaccgtt atctcctggg agaaaatcaa caaagcgcag gcgctgtgga ctcgtaacaa
    16801 gtcggaaatc accgatgaag agtacaaaga gttctacaaa cacatcgccc acgactttaa
    16861 tgatccgctg acctggagcc acaaccgcgt tgaaggtaag caggagtaca ccagcctgct
    16921 gtacattccg tcccaggctc cgtgggatat gtggaaccgt gatcataaac acggcctgaa
    16981 actgtacgtc cagcgtgtgt tcatcatgga cgacgcagaa cagttcatgc cgaactatct
    17041 gcgcttcgtg cgtggtctga ttgactccag cgatctgccg ctgaacgttt cccgtgaaat
    17101 cctccaggac agcacggtaa cgcgtaacct gcgcaatgcg ctgaccaagc gtgtgctgca
    17161 aatgctggaa aaactggcga aagacgacgc ggaaaaatac cagaccttct ggcaacagtt
    17221 tggcctggta ctgaaagaag gtccggcgga agatttcgct aaccaggaag cgatcgccaa
    17281 actgctgcgt tttgcttcta cccataccga ttcttctgcg cagaccgtat ctctggaaga
    17341 ctacgtttcc cgcatgaaag aagggcagga gaaaatctac tacatcaccg cagacagcta
    17401 tgcggcagcg aagagcagcc cgcacctgga actgctgcgt aagaaaggca tcgaagttct
    17461 gctgctttcc gaccgcatcg atgagtggat gatgaactat ctgactgagt tcgacggtaa
    17521 accgttccag tcggtgtcta aagttgacga gtcgctggaa aaactggctg acgaagttga
    17581 tgagagcgcg aaagaagcgg agaaagcact gactccgttc atcgaccgtg tgaaagccct
    17641 gctcggcgag cgcgtgaaag atgtccgtct gactcaccgt ctgaccgata cgccagcgat
    17701 cgtttcgacc gacgcggacg aaatgagcac tcagatggcg aaactgttcg ctgcggcggg
    17761 ccagaaagtg ccagaagtga aatacatctt cgaactgaac ccggatcacg tactggtgaa
    17821 acgtgcggca gatactgaag atgaagcgaa gttcagcgag tgggtagaac tgctgctgga
    17881 tcaggcgctg ctggcagaac gcggcacgct ggaagatccg aacctgttta ttcgtcgtat
    17941 gaaccagctg ctggtttcct gatgtcatgc cggatgacct ttgtgtcatc cggcattttt
    18001 cttttcatca tctgcacttt ccgcaaatta tctcgccatt aaccgtttca gcctcaggtg
    18061 cctttcttga ggcaatcgcc tgttggtggt atcgtttatc gctttttcaa aaaaatcgac
    18121 acattttaag gggattttcg caatgcgtat cattctgctt ggcgctccgg gcgcggggaa
    18181 agggactcag gctcagttca tcatggagaa atatggtatt ccgcaaatct ccactggcga
    18241 tatgctgcgt gctgcggtca aatctggctc cgagctgggt aaacaagcaa aagacattat
    18301 ggatgctggc aaactggtca ccgacgaact ggtgatcgcg ctggttaaag agcgcattgc
    18361 tcaggaagac tgccgtaatg gtttcctgtt ggacggcttc ccgcgtacca ttccgcaggc
    18421 agacgcgatg aaagaagcgg gcatcaatgt tgattacgtt ctggaattcg acgtaccgga
    18481 cgaactgatt gttgatcgta tcgtaggccg ccgcgttcat gcgccgtctg gtcgtgttta
    18541 tcacgttaaa ttcaatccgc cgaaagtaga aggcaaagac gacgttaccg gtgaagaact
    18601 gactacccgt aaagacgatc aggaagaaac cgtgcgtaaa cgtctggttg aataccatca
    18661 gatgactgca ccgctgatcg gctactactt caaagaagcg gaagcgggta acaccaaata
    18721 cgcgaaagtt gacggcacca agtcggttgc tgaagttcgc gctgatctgg aaaaaatcct
    18781 cggctaatca agccgcagca gatctgcttt cgggcaggtc tgctcatcca cctcaggcaa
    18841 tacctctcac agcaattagt tcttcttcct cacttttccg ctacaattat caacaagttg
    18901 aatcgataag aggcggtaat gcgtcagact aaaatcggta tcctgctggc aaacctgggt
    18961 acgcccgata cccccacacc tgaagcggta aaacgctatc tgaaacaatt tttaagcgac
    19021 agacgcgtgg ttgatacctc acggttgtta tggtggccat tgctgcgcgg cgttattttg
    19081 ccgctgcgct cgccgcgtgt ggcgaagctg tacgcctcgg tctggatgga aggtggctcg
    19141 ccgctgatgg tttacagccg ccagcaacag caggcgctgg cacaacgttt accggagacg
    19201 cccgtagcgc tgggaatgag ctacggctcg ccatcactgg aaagcgccgt cgatgaactc
    19261 ctggcagagc atgtagatca tattgtggtg ctgccgcttt atccgcaatt ctcctgttct
    19321 acggtcggtg cggtatggga tgaactggca cgcattctgg cgcgcaaacg tagcattccg
    19381 gggatatcgt ttattcgtga ttacgccgat aaccacgatt acattaatgc actggcgaac
    19441 agcgtacgcg cttcttttgc caaacatggc gaaccggatc tgctgctgct ctcttatcat
    19501 ggcattcccc agcgttatgc agatgaaggc gatgattacc cgcaacgttg ccgcacaacg
    19561 actcgcgaac tggcttccgc actggggatg gcaccggaaa aagtgatgat gacctttcag
    19621 tcgcgctttg gccgggaacc ctggctgatg ccttataccg acgaaacgct gaaaatgctc
    19681 ggagaaaaag gcgtaggtca tatacaggtg atgtgcccgg gctttgctgc ggattgtctg
    19741 gagacgctgg aagagattgc cgagcaaaac cgtgaggtct tcctcggtgc gggcgggaaa
    19801 aaatatgaat atattccagc gcttaatgcc acgccggaac atatcgaaat gatggctaat
    19861 cttgttgccg cgtatcgcta aagctgagcg gtaaagaact gagcgccgtc gcgaagagcc
    19921 tcgtcggcgg ttttcatcat ccgtgaataa tgcaaaaagg catgcagcgt acctgggtag
    19981 agtttgaact cacagggctg ctgatgcgcc gctaacgtct ggtaaagcag acggctgtca
    20041 tccagcagcg gatcgaactc cgccccggca ataaaacagg gcggaacttc gcgagtgaga
    20101 tcattattaa acagacagta atacggtgac tcgcggtccg cgtcgttgct taaatacgcc
    20161 tcttcataca tctgcaaatc ctgttgcgtt aagccatccc agacaccgcc caacagacga
    20221 cgagtcacgg aatcccgtaa tccgtaaagc ccataccaca gcaaaacacc cgcaatttta
    20281 ccgcaatcga tctgtttatc acgcaaccac aacgcactgg cgagcgccag catggcacct
    20341 gcggaatcac cggcaaagcc aatgcgggac atattgattt gataatcctc cgcctgctgg
    20401 tggaaataac aacaagcagc cacaatttcc tctatcgctt gcggaaaacg cgcttcaggt
    20461 gaaagggtgt aatcaatacc aatcaccgta cattggctgt agcttgccag caggcgcatg
    20521 atgcgatcgt gggtatcgag attgccgaga ataaaaccgc ctccatgcaa ataaaatagc
    20581 gtcgccgggc tatctggctg cggacaaaag agacgtgttt ccacctgccc atattttgtt
    20641 ggaaccatgt aagctctggt tgccatttct ggagcgcccg cattccagaa tcggcgctca
    20701 agcgtgtaat actgtcgttg ctcagcaatc gttcccgttg cgggccagga cggtaaatcc
    20761 ggctgaagag tattcacaac ggtcttcatt tcagcagaaa taaggtccag aacaggtagt
    20821 ttgttttccg gcttcataca aaaactcctt tcaaattacg tcattgtaag gaaccactgc
    20881 catgaaaatg cgatcccgcc tgctgatatt gaaactggct gcgtctcgcg cgctcccgtc
    20941 agattgtgtt aacattcgcc gctcagttaa ccacccgtaa aaacaaccat gaaatttccc
    21001 ggtaaacgta aatccaaaca ttacttccct gtaaatgcac gcgatccgct gcttcagcaa
    21061 ttccagccag aaaacgaaac cagcgccgcc tgggtcgtag gtattgacca gacgctggtc
    21121 gatattgaag cgaaagtgga tgatgaattc attgagcgtt atggattaag cgccgggcat
    21181 tcactggtga ttgaggatga cgtagccgaa gcgctttatc aggagctaaa acagaaaaac
    21241 ctgattaccc atcagtttgc gggtggcacc attggtaaca ccatgcacaa ctactcggtg
    21301 ctcgcggacg accgttcggt gctgctgggc gtcatgtgca gcaatattga aattggcagt
    21361 tatgcctatc gttacctgtg taacacttcc agccgtaccg atcttaacta tctacaaggc
    21421 gtggatggcc cgattggtcg ttgctttacg ctgattggcg agtccgggga acgtaccttt
    21481 gctatcagtc caggccacat gaaccagctg cgggctgaaa gcattccgga agatgtgatt
    21541 gccggagcct cggcactggt tctcacctct tatctggtgc gttgcaaacc gggtgaaccc
    21601 atgccggaag caaccatgaa agccattgag tacgcgaaga aatataacgt accggtggtg
    21661 ctgacgctgg gcaccaagtt tgtcattgcc gagaatccgc agtggtggca gcaattcctc
    21721 aaagatcacg tctctatcct tgcgatgaac gaagatgaag ccgaagcgtt gaccggagaa
    21781 agcgatccgt tgttggcatc tgacaaggcg ctggactggg tagatctggt gctgtgcacc
    21841 gccgggccaa tcggcttgta tatggcgggc tttaccgaag acgaagcgaa acgtaaaacc
    21901 cagcatccgc tgctgccggg cgctatagcg gaattcaacc agtatgagtt tagccgcgcc
    21961 atgcgccaca aggattgcca gaatccgctg cgtgtctatt cgcacattgc gccgtacatg
    22021 ggcgggccgg aaaaaatcat gaacactaat ggagcggggg atggcgcatt ggcagcgttg
    22081 ctgcatgaca ttaccgccaa cagctaccat cgtagcaacg taccaaactc cagcaaacat
    22141 aaattcacct ggttaactta ttcatcgtta gcgcaggtgt gtaaatatgc taaccgtgtg
    22201 agctatcagg tactgaacca gcattcacct cgtttaacgc gcggcttgcc ggagcgtgaa
    22261 gacagcctgg aagagtctta ctgggatcgt taagttatcg tcggttcgta ggccagataa
    22321 ggcgttcacg ccgcatctgg catttggctc tcgatgcctg atgcgacgct ggcgcgtctt
    22381 atcatgccta catatttttc atattttaca tccggcaacc accgtttacc ccgtcaccac
    22441 ttcacccgcc ggtggcgttt ccagcagttc cagcatagta cgggcaattt cacgttcacc
    22501 catcactacc tgattcgcgc cacgttcggt gatatacgtc acttcatcgt cataatgggc
    22561 gcgggcgata atctcaatat ccgggttttt cgcgcgggca gacgccacaa tctcacccgc
    22621 ttcataaccg ttggggatcg tcaggatcag ccattttgca cattccagat gcgccagttg
    22681 cataatttct tcgttcgctg cattgcccaa tacagcgcgg accccacgct ctcgcagctc
    22741 atcaacacgg gttcgtgacg tctcaatcac caccagcgga atatcagagg cgagcaattt
    22801 ctcccccagc aggctgccta cacgaccgta acccaccaat agcgcatggt tgcaaatatc
    22861 cactgggatc tgtttctctt cttcgattgc ctcttccagc gtctgctctt ccagcgtttc
    22921 ggtcttcgcc agatatttct ccagtagtgc gaacagtacc gggttgagca taatcgacag
    22981 gatcgcccct gccagtacca ggttttgtcc ggcctgcggc agtaaattca atgccattcc
    23041 cagtcccgcc aggataaacg cgaactcacc aatctgcgcc aggctggcgg cgatggttaa
    23101 tgccgtacgt tgggagtgac caaacagacg gacaaggaac agggccgcga ccgatttacc
    23161 aaacagaata attgccagcg tcgccagcac tgccagcggt tgctgaatca gaattaacgg
    23221 atcaaacaac atcccgacgg agacaaaaaa cagcaccgca aacgcgtcgc gcaatggcag
    23281 cgtatcgtgg gcggcacggt gactcagttc agactcgttc agtaccatcc cggcaaagaa
    23341 cgcgccaagt gcgaaggaga cgtcaaacaa ctccaccgca ccaaaggcaa tccctaacgc
    23401 cagcgccagc accgacaggg taaatagctc gcgagaaccg gttgccgcgc tgcgtgccat
    23461 aatccacggc accagacggc gacctaccag catcataatg gcgataaatg cgatcacttt
    23521 gccgatggtg atccccatat cgactgcaag agtggcaaag cccacatcgc cctgttccat
    23581 cattcctgcc actgcgggca gcaacaccag cgtcagaacc attaccaggt cttccacaat
    23641 caaccaaccg atggcgattt gcccacgctg actgtcaatt aattgccgtt cttcaagtgc
    23701 gcgcagtaac accacggtac tggcggtgga aagacataaa ccgaacacga taccggtcat
    23761 taacgaccag cccagcacgg cagagagcgc catacccagc agcgtcgcca cggctatctg
    23821 ggcgatcgca ccgggaatgg cgatggcctt taccgccatc aaatccttca gcgaaaagtg
    23881 caaaccgacg ccaaacatca acagaatgac gcccagttca gccagttccg gcgcaagctt
    23941 ggtatcggca acaaagcccg gagtgaatgg tcctgccagc acacccgcta acagatatcc
    24001 caccagagga gaaatacgta gtttattggc cagcatgccg aggataaagg cgagcacaag
    24061 gccgccaaca atggtggtga taagcggggt ggcgtgatgc attccgtctc cttttcctgg
    24121 tggttattgt ccatttttgg ccgggaaaac caaaattaca ggtaatagtt tatgacaatt
    24181 tcattgatga tgttcatgaa taattattga attttgcaga aaaatggaat tagctgcaaa
    24241 aaaagcacgg atcggaaaac agaaggcggt ttaacagagg aaaggcttat ggcgactgta
    24301 aaacgatgca gccaaagttt gcctttggct gcaatgaaat cagtctttat gccggttatc
    24361 aggcaggaat atggtcaaca tccccaatag tggcaggaaa gcacagattt tatagactaa
    24421 ctcgatgct
//
LOCUS       DAESHI010000058        23939 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.058, whole genome shotgun sequence.
ACCESSION   DAESHI010000058 DAESHI010000000
VERSION     DAESHI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.058"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(38..400)
                     /locus_tag="KW011_002403"
     CDS             complement(38..400)
                     /locus_tag="KW011_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBH7660714.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            937..2850
                     /gene="mtlA"
                     /locus_tag="KW011_002404"
     CDS             937..2850
                     /gene="mtlA"
                     /locus_tag="KW011_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBH7660715.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3080..4228
                     /gene="mtlD"
                     /locus_tag="KW011_002405"
     CDS             3080..4228
                     /gene="mtlD"
                     /locus_tag="KW011_002405"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBH7660716.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4228..4815
                     /gene="mtlR"
                     /locus_tag="KW011_002406"
     CDS             4228..4815
                     /gene="mtlR"
                     /locus_tag="KW011_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HBH7660717.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4827..5036)
                     /locus_tag="KW011_002407"
     CDS             complement(4827..5036)
                     /locus_tag="KW011_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660718.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5321..5683
                     /locus_tag="KW011_002408"
     CDS             5321..5683
                     /locus_tag="KW011_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HBH7660719.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            6226..6904
                     /locus_tag="KW011_002409"
                     /pseudo
     CDS             6226..6904
                     /locus_tag="KW011_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
     gene            6948..11798
                     /locus_tag="KW011_002410"
     CDS             6948..11798
                     /locus_tag="KW011_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HBH7660720.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDSWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKTLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEENGAGINYVRTN
                     DNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALGSSSVSSRVIA
                     KGSRDTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAI
                     NGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            12166..13821
                     /gene="lldP"
                     /locus_tag="KW011_002411"
     CDS             12166..13821
                     /gene="lldP"
                     /locus_tag="KW011_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HBH7660721.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            13821..14597
                     /gene="lldR"
                     /locus_tag="KW011_002412"
     CDS             13821..14597
                     /gene="lldR"
                     /locus_tag="KW011_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HBH7660722.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            14594..15784
                     /gene="lldD"
                     /locus_tag="KW011_002413"
     CDS             14594..15784
                     /gene="lldD"
                     /locus_tag="KW011_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HBH7660723.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            15968..16441
                     /gene="trmL"
                     /locus_tag="KW011_002414"
     CDS             15968..16441
                     /gene="trmL"
                     /locus_tag="KW011_002414"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBH7660724.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(16494..17315)
                     /gene="cysE"
                     /locus_tag="KW011_002415"
     CDS             complement(16494..17315)
                     /gene="cysE"
                     /locus_tag="KW011_002415"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBH7660725.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(17395..18414)
                     /gene="gpsA"
                     /locus_tag="KW011_002416"
     CDS             complement(17395..18414)
                     /gene="gpsA"
                     /locus_tag="KW011_002416"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBH7660726.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(18414..18881)
                     /gene="secB"
                     /locus_tag="KW011_002417"
     CDS             complement(18414..18881)
                     /gene="secB"
                     /locus_tag="KW011_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HBH7660727.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(18944..19195)
                     /gene="grxC"
                     /locus_tag="KW011_002418"
     CDS             complement(18944..19195)
                     /gene="grxC"
                     /locus_tag="KW011_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HBH7660728.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(19337..19768)
                     /locus_tag="KW011_002419"
     CDS             complement(19337..19768)
                     /locus_tag="KW011_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBH7660729.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            20013..21557
                     /gene="gpmM"
                     /locus_tag="KW011_002420"
                     /gene_synonym="pgmI"
     CDS             20013..21557
                     /gene="gpmM"
                     /locus_tag="KW011_002420"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBH7660730.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            21567..22850
                     /gene="envC"
                     /locus_tag="KW011_002421"
     CDS             21567..22850
                     /gene="envC"
                     /locus_tag="KW011_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HBH7660731.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            22854..23813
                     /locus_tag="KW011_002422"
     CDS             22854..23813
                     /locus_tag="KW011_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HBH7660732.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPNNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
     gene            complement(23800..>23939)
                     /locus_tag="KW011_002423"
     CDS             complement(23800..>23939)
                     /locus_tag="KW011_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBH7660733.1"
                     /translation="YKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHHRITRSAFNL
                     R"
BASE COUNT         6042 a         6102 c         6211 g         5584 t
ORIGIN      
        1 agcaccacgt aagttaaaac aatcaataga tccataatta gatctccggg aacgtggaat
       61 agtcagtctt tttctcggcg gcgagttgat gctctagccg ggaaattcgg tcagaaagtg
      121 cggcgatttc cgggtcagtc gctttctcct gcgacgcaac atgtgactgc atgccccacc
      181 cgcgctccgg ttgatagagc gtcgcccaga tccacaggaa cggccagata acatgcaggg
      241 taaacaggct cacccagccc gccgtatgaa tagcgtcggc atggggatgg ttgcgctttt
      301 tggcaatcag ataagggatg tcatgtatcg cgatgattcc ataaaaaatc accagaaaga
      361 cgaagatcag cactcccaac gcgaaatagt ttagaaacat atctgcctcg gattcacgtt
      421 tatcagtgtt gtttttgggc tggcagccag aagggagtca ggctgatatt ttgacaataa
      481 tccgggttcg cgattctcgc cataacacca aagaataatt tttagaggtg atgagttgct
      541 tagttacata acaattgtat gacgaaggca taacatgctg tagatcacat caggtgaacg
      601 ccgtaagaaa atatcttgtg attcagatca caaagattca acaaaccatc aaaacaaaaa
      661 tgtgacacta ctcacattta aatgccattt ttagcgaaaa tcgccgcctt gttgcttttt
      721 tacacaagcg ttttgtgatg aacgtcacgt taattacctc tctaccccct atatttatgt
      781 gattgatatc acacaaaagg ccgtcgactg gacagttaac cgattcagtg ccagatttcg
      841 cagtatctac aaggtccggc tacctctgcc gccacattaa caaaaaacct cgggcttcca
      901 gcctgcgcga cagcaaacat aagaaggggt gtttttatgt catccgatat taagatcaaa
      961 gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc caaatatcgg cgcgtttatc
     1021 gcgtggggta tcatcaccgc gttatttatt ccaacagggt ggttaccgaa cgagacgctg
     1081 gcgaagctgg tcgggccgat gatcacttat ctcctgccgc tgctgatcgg ttataccggt
     1141 ggtaagctgg taggcggcga acgtggcggc gtagtcggtg ccatcaccac catgggcgtt
     1201 atcgtcggcg cagacatgcc gatgttcctc ggttctatga ttgcaggtcc gctgggcggc
     1261 tggtgcatta agcacttcga ccgctgggta gacggtaaga tcaaatccgg ttttgagatg
     1321 ctggtgaata acttctccgc aggcatcatc gggatgatcc tcgctattct ggcattcctc
     1381 ggcattggcc cgattgttga agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg
     1441 gttgtccatg acatgctgcc gctggcgtct atctttgttg aaccggcgaa aatcctgttc
     1501 ctcaacaacg ccattaacca cggtatcttc tcgccgctgg gtattcagca gtcccatgaa
     1561 ctgggtaaat caatcttctt cctgattgaa gctaacccag gtccaggtat gggcgtgctg
     1621 ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc
     1681 atccacttcc tgggtggtat ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt
     1741 ctgatcctcg cagtcatcct cggcggtatg actggcgtgt tcacgctgac tatcctgggc
     1801 ggtggtctgg tttctccggc atctccgggt tctatccttg ctgtactggc gatgacacca
     1861 aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt
     1921 gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag aagatgatat tgaagcagca
     1981 actcgtcgta tgcaggacat gaaagctgag tctaaaggca catctccgct gtctgcgggc
     2041 gatgtgacta acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg
     2101 ggttccagtg cgatgggcgc aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtca
     2161 cagatttcgg tcactaacag cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc
     2221 actcaccgtg acctgaccga acgcgctatg cgccaggttc cgcaggcgca gcatatttcg
     2281 ctgaccaact tcctcgacag cggcctgtac accagcctaa ccgaacgtct ggttgctgcc
     2341 cagcgccata ctgaaaacga agtgaaagtg aaagacagtc tgaaagacag ctttgacgat
     2401 tccagtgcta acctgttcaa actgggcgcg gagaacatct tcctcggtcg caaagcagca
     2461 accaaagaag aagcgattcg ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag
     2521 ccggaatacg ttcaggcgat gctggatcgt gaaaaactga ccccgactta tctgggtgag
     2581 tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtg
     2641 gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag aagaagatga cattgcccgt
     2701 ctggtgattg gtattgcagc ccgtaacaac gagcacattc aggttatcac cagcctgacc
     2761 aatgcactgg atgatgagtc cgtcatcgag cgtctggcac acaccaccag cgtggatgaa
     2821 gtgctggaac tgttggcagg tcgtaagtaa tccaatccca ccctctccac atggagaagg
     2881 tggggttaat tgcctgatgc gctacgctta tcaggcctac aggatgcatc acgatttgtt
     2941 gaatttgcac gttcttgtag gccggataag gcgcttacgc cgcatccggc gctgcccctc
     3001 tcctcacgga gagggtttgg gtgagggaaa agcctcaccc cagccctctc gggtaaaaac
     3061 attgatgaag gttaatacta tgaaagcatt acattttggc gcaggtaata tcggtcgtgg
     3121 ctttatcggt aaactgctgg cagacgcggg tatccaactg acgtttgccg atgtcaatca
     3181 ggtggtactt gatgccctga atgcccgtca tagctatcag gttcatgtgg ttggtgaaac
     3241 cgagcaggta gataccgttt ccggcgtcaa tgctgtcagc agcattggtg atgatgtcgt
     3301 tgatctgatt gctcaggtcg atttagtcac taccgccgtt ggcccggttg tgctggaacg
     3361 tattgctccg gctatcgcca aagggctggt gaaacgcaaa gaacaaggta atgaatcccc
     3421 gctgaacatc atcgcctgtg aaaacatggt acgcggtacc acgcagctga aaggccatgt
     3481 gatgaacgcc ctgccggaag acgccaaagc gtgggtagaa gaacacgttg gctttgtcga
     3541 ttccgccgtt gaccgcatcg taccgccttc ggcttcggca actaacgatc cgctggaagt
     3601 gacggtagaa actttcagcg aatggattgt cgataaaacg cagttcaaag gcgcactgcc
     3661 gaacatccca ggcatggagt taaccgacaa cctgatggca tttgtcgaac gtaaactctt
     3721 caccctgaac acgggtcatg ctataaccgc gtacctcgga aaactggccg gtcatcagac
     3781 cattcgtgac gctattctcg acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga
     3841 aagtggtgcg gtactgatca agcgctacgg ctttgacgca gacaaacatg cggcgtacat
     3901 ccagaaaatc ctcggtcgtt ttgagaaccc gtatctgaaa gatgatgtag agcgcgtagg
     3961 ccgtcagccg ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccat tgctcggtac
     4021 gctggaatac agccttccgc acaagaatct gattcagggg attgctggtg caatgcactt
     4081 ccgcagtgaa gatgatccgc aggctcagga actggcagca ctgatcgctg acaaaggtcc
     4141 gcaggcggcg ctggcacaga tttccgatct tgatgccaac agcgaggttg tatccgaggc
     4201 ggtaaccgct tataaagcaa tgcaataatg gtggaccagg cgcaggacac cctgcgcccg
     4261 aataacagat tgtcagatat gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt
     4321 gtgcttgagc gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag
     4381 cttttgaccg aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga cgattacgcg
     4441 gtgaagtatg ctgtagaacc gttactcgac ggcgacggtc cgctgggcga tctttctgtg
     4501 cgtttaaaac tcatttacgg gttgggcgtc attaaccgcc aggaatacga agatgcggag
     4561 ctgctgatgg cattgcgtga agaactaaat cacgacggca acgagtacgc ctttaccgac
     4621 gacgagatcc ttggaccctt tggtgaactg cactgcgtgg cggcgttacc accgccgcca
     4681 cagtttgaac cagcagactc cagtttgtat gcaatgcaaa ttcagcgcta tcaacaggct
     4741 gtgcgatcaa caatggtcct ttcactgact gagctgattt ccagaatcag cttaaaaaaa
     4801 gcctttcaaa agtaagcaac gtctgcttac tgcccctcta cctgcttcgg ccgataaagc
     4861 cgacgataat actccagacg ttgtagatat aacggcacgc tttcatcggg tattccggac
     4921 ggaatcgcgt tacggggagg aagttttttc agatactccc ggaacgcctg gcttgatgcc
     4981 atgaaatcta cggctttatc gataagaagc ggaacgtttt cacctaattt gcccatgatg
     5041 atatctccgt attaccccgc gccgggaatg cgcggccgcc aattttagtt taggttcccg
     5101 gtaaagatga cattaaggaa aacgtgctga atcctcaata atcatccgct gctgtttatt
     5161 atcttttttc tatagatttc ttaattaatc aataaattgc gtagttttta agcatgttaa
     5221 tgattacgtt acagaactat tgcaatgtca tcagttgtga acaaagcacc tggtcgcgca
     5281 tactaggggc tataaattta tctttatcag aagccatcac atgaaagaag tcgaaaaaaa
     5341 cgaaatcaaa cgtctcagcg atcgtctgga cgccatccgc caccagcagg ccgatctgtc
     5401 gctggttgaa gccgcagaca aatatgccga gctggaaaaa gagaaagcca cgctggaagc
     5461 agaaattgct cgcctgcgtg aagttcatag ccaaaaactg agtaaagaag cacagaagct
     5521 gatgaagatg ccgtttcagc gcgcaattac caaaaaagag caggctgata tgggcaagct
     5581 gaagaaaagt gttcgcggac tggtggttgt gcacccaatg accgcgctgg gccgtgaaat
     5641 gggcctgcag gagatgactg gattctctaa gactgcgttt taagcgcctc agacgactgg
     5701 ttcgatcata tcaacccgca tctggcttgc cttagctggt caatgcgggg actctcctgt
     5761 ctactcgcct aattaccaaa caattcactc cctttacgtc gatttaccgc tggttgatat
     5821 tctgaaaatg catttttacc gaaatagcca cctataaaca ataaaaatat atagaagata
     5881 tcatttatta attaacaagc agttacgtct ctttttttcg gatttaattg tgaaatttta
     5941 atcatcatat tcatacaata aattatctgt aaattttttc ccagacatgg tttctttcat
     6001 atttgacatt tattaataat catcattaga atccacgaaa ataatatatt catacaaaca
     6061 cagacatctt ttgaaacgaa tgatacattt ttatgatgaa tgcttaaaac aatatttaat
     6121 cgacacacat cactgtcatt aatgtaaagc aacatagatc accaataata caatggacaa
     6181 ctctaccggc attgatcgct ggtcggagga aataaggatt tttacatgaa cttgaagaag
     6241 atttttttca gtgctgtcac tgtttcagtt ctctgcgcat taacgggatg tgactacatt
     6301 gaagaaggca agccagaaag tagtttattg aaacaacaag aagaacatta aaattgatct
     6361 acttgaaaaa caacaagcac agctaaagag tcaactggaa actattcaaa aacaacaaac
     6421 tggcataata agcagtacaa agactttaac tcacgtaatt aaatccgtta aagatcaaca
     6481 aaacactttt atttttaccg agtttaaccc agcaaaaacg aagtatttca tcctgaacaa
     6541 tggttcggtg gctttagcgg gtcgagtgtt atccattgac gccacagaga atggcagtgt
     6601 tattcatatt tcactggtca acttattaag tacacctatc tcaaatattg gttttaatgc
     6661 gacatggggt ggtgaaaaac ctgtcgatgc caaagagttt gcccggtggc aacaattact
     6721 tttcaacaca tcaatgaaat ccacattgaa attattacca ggtcaatggc aagacattaa
     6781 tttgaccctg aagggtgtat cgcccaataa cctgggatat ctgaagttag ccatcaacat
     6841 ggaaaatatt cagttcgaca atcttccctc tgctgaaaat cggcagaaaa gaagcaaaaa
     6901 ataaatgcag ttagtacgct tataacaagg aatagcgagc atcattaatg aacaaaatat
     6961 ttaaagttat ctggaaccct gcgacaggga attatactgt taccagcgaa acggcaaaaa
     7021 gccgtggcaa gaaatctggg cgcagtaagc tgttaatttc tgcgctggtt gcgggtggaa
     7081 tgttgtcgtc gtttggggca ttggcgaatg ccgggaatga caacggccag ggtgttgatt
     7141 acggtagtgg atcagctggc gacagctggg ttgctatagg caaaggggcg aaagcaaata
     7201 cttttatgaa caccagtggt tccagtactg ctgtgggtta tgacgctata gctgaaggcc
     7261 aatatagctc tgccatcggg tcaaaaaccc atgcgattgg tggtgcatca atggcctttg
     7321 gggttagtgc aatatcagaa ggcgatagaa gtatagcact gggtgcctct tcgtattcat
     7381 tgggccaata ctcaatggcc ctcggccgtt attcaaaagc attgggtaaa ttgtctattg
     7441 ctatggggga ctcttccaaa gcggaaggag caaacgccat tgccctggga aatgccacta
     7501 aagctactga gattatgagt attgctcttg gcgacaccgc caatgcgtca aaagcgtatt
     7561 caatggcgct gggagcaagt agcgtcgcat ctgaagaaaa cgcaattgcc ctggggcgta
     7621 gcagtgtagc tagcggtact gacagcctcg catttggcag acaatcactt gccagcgcag
     7681 cgaacgctat tgcgataggt gctgagaccg aagccgctga aaatgcaact gctattggca
     7741 ataatgcgaa ggcaaaaggg actaatagca tggcaatggg gttcggaagc cttgccgata
     7801 aagtcaatac tatcgcatta ggaaatggca gccaggctct ggcagataat gcaatcgcca
     7861 taggccaggg caacaaagct gatggcgtgg atgccatcgc tctgggtaat ggtagccagt
     7921 cgagaggctt aaacaccatt gccttaggca cagccagtaa tgcaactggt gataagagtc
     7981 ttgcgcttgg tagtaatagc agtgccaacg gtattaactc tgtcgcgctg ggcgcagatt
     8041 ccattgcgga tttagacaat accgtctctg tcggcaatag ttcattaaaa cgcaagatcg
     8101 ttaatgtgaa aaatggcgcg atcaagtctg acagttacga tgccattaat ggttcacagc
     8161 tttatgccat tagcgactcg gtagcaaaaa cgcttggagg aggggctgca gtagatgttg
     8221 atgacggtac tgttacagca ccaacctaca atttaaaaaa tggtagcaaa aataacgtag
     8281 gggctgcgct cgctgtactt gatgaaaaca ccctgcaatg ggaccaaacc aaaggcaaat
     8341 acagcgctgc tcatggtact agtagcccaa ctgccagcgt aatcaccgat gttgcggatg
     8401 gcacgatttc agcctccagt aaggatgcgg ttaacggttc ccaactgaaa gctaccaatg
     8461 acgatgtcga agccaacacc gccaatatcg ctactaatac cagcaacatt gccacgaata
     8521 cagcaagtat tgccaccaat accaccaata tcaccaacct gacggattcc gttggtgacc
     8581 ttcaggctga tgccctgctc tggaacgaaa ctaaaaaggc attcagtgca gctcacggcc
     8641 aggataccac cagcaaaatc accaacgtta aagatgccga cctgacggct gacagcactg
     8701 atgctgttaa cggctctcag ctgaaaacca ccaacgatgc tgtggcgacg aataccacca
     8761 atatcgccaa taacacttcc aatattgcca ctaacaccac caacatctct aatctgactg
     8821 agacggtgac taatcttggt gaggatgcgc tgaaatggga taaggacaat ggtgtattca
     8881 cggcagctca tggcaccgag accaccagca aaatcaccaa cgttaaagat ggcgacctga
     8941 cgactggcag caccgatgcc gttaacggct ctcagctgaa aaccaccaac gatgccgtgg
     9001 cgacgaatac caccaatatc gccactaaca ccaccaacat ctctaatctg actgagacgg
     9061 tgactaatct tggtgaggat gcgctgaaat gggataagga caatggtgtc ttcactgcag
     9121 ctcatggcaa caataccgcc agcaaaatca ccaatatcct ggacggcaca gtcactgcaa
     9181 ccagttccga tgccattaac ggtagccagc tttatgactt aagcagcaat atcgccacct
     9241 acttcggcgg caatgcttct gtgaatactg acggtgtgtt taccggtcca acctacaaaa
     9301 tcggtgaaac aaattattat aacgtcggcg atgcactggc tgcgattaac tcctcattta
     9361 gcacgtctct cggcgatgct ctgctttggg atgccaccgc aggtaaattc agtgccaaac
     9421 acggtactaa tggtgacgca agcgtgatca ctgatgtcgc agatggtgaa atttcagact
     9481 ccagttctga cgcagtaaac ggctcacaac tccacggcgt gagcagttat gttgttgatg
     9541 cgctgggggg tggtgccgaa gtcaatgcag acggcaccat cactgcgccg acgtacacca
     9601 ttgctaatgc tgattacgat aatgtcggtg atgccctgaa tgctatcgat accactcttg
     9661 acgacgctct gctctgggat gcggacgccg gtgaaaatgg tgcatttagc gccgctcacg
     9721 gaaaagataa aactgccagt gtaatcacta acgtcgctaa cggtgcaatc tctgctgcca
     9781 gcagcgacgc gattaacggc tcacaactct ataccaccaa taagtacatc gctgatgcgc
     9841 tgggtggtga cgcagaagtc aacgctgacg gcaccatcac cgcaccgact tacaccattg
     9901 cgaacgccga gtacaacaac gtcggtgacg ccctggatgc gcttgatgat aacgccctgc
     9961 tgtgggatga gactgccaat ggcggtgctg gagcctacaa tgccagccat gacggtaaag
    10021 ccagcatcat cactaatgtc gctaatggca gtattagtga ggacagtacc gatgcagtga
    10081 acggttctca gttgaatgcg acgaatatga tgattgagca gaacacccaa attatcaatc
    10141 agctcgctgg taacaccgac gcaacctata tcgaagaaaa tggcgctggt atcaactacg
    10201 ttcgtactaa cgacaacgat ttagccttta acgatgcaag cgcctctggt gtcggcgcta
    10261 cagctgtagg ttataacgct gtcgcgtctg gtgccagcag cgtagccatt ggtcagaaca
    10321 gcagcagcac cgttgatacc ggtattgcgc tgggtagcag ctccgtttcc agccgtgtga
    10381 ttgccaaagg ttctcgtgac actagcgtaa cggaaaatgg cgtggtgatt ggttatgaca
    10441 ccactgacgg cgaactgcta ggtgcattgt caattggtga tgacggtaaa taccgccaaa
    10501 tcatcaacgt agctgatggt tcagaagccc atgacgccgt tacggttcgc cagttgcaga
    10561 acgctattgg agcggtcgcc actacgccaa ccaagtactt tcacgccaac tcaacggcag
    10621 aagactcact ggccgttggt gaagactcgc tggcaatggg tgcgaaaact gtcgttaatg
    10681 gtaatgcagg tattggtatc ggtttgaaca ctctggttct agctgatgcg atcaacggca
    10741 ttgctatcgg cagtaacgca cgcgcaaacc atgcaaacag tatcgcaatg ggtaacggtt
    10801 ctcagaccac ccgtggtgca cagactggct acaccgccta caacatggac gcaccgcaga
    10861 actctgtagg tgagttttct gtcggtagtg aagacggtca acgtcagatc acaaacgtcg
    10921 cagctggttc agcggatacc gatgcggtta acgtgggtca gttgaaagtc actgatgagc
    10981 gcgtagcgca aaatacccag agcattacta acctgaacaa tcaggtcact aatctggata
    11041 ctcgcgttac taatatcgaa aacggtattg gcgacattgt caccaccggt agcaccaagt
    11101 acttcaagac caacaccgat ggcgtagatg ccaacgccca gggtaaagat agcgttgcta
    11161 ttggttctgg ttccattgct gccgctgaca acagcgtcgc actgggtacc ggttccgttg
    11221 cagaggaaga aaatacaatc tctgtaggtt cttctactaa ccaacgccgt atcaccaacg
    11281 tagctgccgg tgttaatgcc accgatgcgg ttaacgtgtc gcagctgaaa tcttctgaag
    11341 caggcggcgt gcgctacgac accaaagctg atggttctat cgactatagc aatatcaccc
    11401 tcggtggtgg caacggcagt acgactcgta tcagcaacgt ctccgctggc gtcaacaaca
    11461 acgacgcggt gaactacgcg cagttgaagc aaagcgtgca ggaaacgaag caatacaccg
    11521 atcagcggat ggttgagatg gataacaaac tgtctaaaac tgaaagcaag ttgagcggtg
    11581 gtatcgcttc tgcaatggca atgaccggtc tgccgcaggc ttatacaccg ggtgccagca
    11641 tggcttctat tggtggcggt acttacaacg gtgaatcggc agttgcttta ggtgtatcga
    11701 tggtgagcgc caatggtcgt tgggtctaca aattacaagg tagtaccaat agccagggtg
    11761 aatactccgc cgcactcggt gccggtattc agtggtaatg atccattaag ttagtgtgac
    11821 taagggcaaa cgccaaattg cctgatgcgt tacgcgcatc aggcaagata aggcgccccg
    11881 ggtcaacaat ctaagggcaa ttctctgatg aggattgccc ttttctttac cagacatctc
    11941 cccccacaag aattggccct accaattctt cgcttatctg acctctggtt cacaattccc
    12001 caattaaaac tcacatcaat attgccaata cataacattt agttaactat tcattgtcat
    12061 tatccctaca caacacaatt ggcagtgcca cttttacaca acgtgtgaca aggagatgag
    12121 caacagactc attacacgat gtgcgtggac tccaggagac ctgcaatgaa tctctggcaa
    12181 caaaactacg atcccgccgg gaatatctgg ctttccagtc tgatagcatc gcttcccatc
    12241 ctgtttttct tctttgcgct gattaagctc aaactgaaag gatacgtcgc cgcctcatgg
    12301 acggtagcaa tcgcccttgc cgtggctttg ctgttctata aaatgccggt cgctaacgcg
    12361 ctggcctcgg tggtttatgg cttcttctat ggtttgtggc ccatcgcgtg gatcattatt
    12421 gcagcagtgt tcgtctataa gatctcggtg aaaaccgggc agtttgacat cattcgctcg
    12481 tctattcttt cgataacccc tgaccagcgt ctgcaaatgc tgatcgtcgg tttctgtttc
    12541 ggcgctttcc ttgaaggagc cgcaggcttt ggcgcaccgg tagcaattac cgccgcattg
    12601 ctggtcggcc tgggttttaa accgctgtac gccgccggac tgtgcctgat tgttaacacc
    12661 gcgccagtgg catttggcgc gatgggcatt ccaatcctgg ttgccggaca agtaacaggc
    12721 atcgacagct ttgagattgg ccagatggtg gggcggcagc taccgtttat gaccattatc
    12781 gtgctgttct ggatcatggc gattatggac ggctggcgcg gtatcaagga gacgtggcct
    12841 gcggtcgtgg ttgcgggcgg ctcgtttgcc atcgctcagt accttagctc taacttcatt
    12901 gggccggagc tgccggacat tatctcttcg ctggtatcac tgctctgcct gacgctgttc
    12961 ctcaaacgct ggcagccagt gcgcgtattc cgttttggtg atttgggggc gtcaaagatt
    13021 gatatgacgc tggcccacac cggttacact gcgggtcagg tgttacgtgc ctggacaccg
    13081 ttcctgttcc tgacagctac cgtaacactg tggagcatcc cgccgtttaa agccctgttc
    13141 gcatcgggtg gtgcgctgta tgagtgggtg atcaacattc cggtgccgta cctcgataaa
    13201 ctggttgccc gtatgccacc agtggtcagc gaggctacag cctatgccgc cgtgtttaag
    13261 tttgactggt tctctgccac cggcaccgcc attctgtttg ctgcactgct ctcgattgtc
    13321 tggctgaaga tgaaaccgtc tgacgctatc agcaccttcg gcagcacgct gaaagaactg
    13381 gctctaccca tctactccat cggcatggtg ctggcgttcg cctttatctc gaactattcc
    13441 ggactgtcat caacactggc actggcactg gcgcacaccg gtcatgcatt caccttcttc
    13501 tcgccgttcc tcggctggct gggggtattc ctgaccgggt cggatacctc atctaacgcc
    13561 ctgttcgccg cgctgcaagc caccgcagca caacaaattg gcgtctctga tctgttgctg
    13621 gttgccgcca ataccaccgg tggcgtcacc ggtaagatga tctccccgca atctatcgct
    13681 atcgcctgtg cggcggtagg cctggtgggc aaagagtctg atttgttccg ctttactgtc
    13741 aaacacagcc tgatcttcac ctgtatggtg ggcgtgatca ccacgcttca ggcttatgtc
    13801 ttaacgtgga tgattcctta atgattgttt tacccagacg cctgtcagac gaggttgccg
    13861 atcgtgtgcg ggcgctgatt gatgaaaaaa acctggaagc gggcatgaag ttgcccgctg
    13921 agcgccaact ggcgatgcaa ctcggcgtat cacgtaattc actgcgcgag gcgctggcaa
    13981 aactggtgag cgaaggcgtg ctgctcagtc gacgcggcgg cgggacgttt attcgctggc
    14041 gtcatgacac atggtcggag caaaacatcg tccagccgct aaaaacgctg atggccgatg
    14101 atccggatta cagtttcgat attctggaag cccgctacgc cattgaagcc agcaccgcat
    14161 ggcatgcggc aatgcgcgcc acacctggcg acaaagaaaa gattcagctt tgctttgaag
    14221 caacgctaag tgaagacccg gatatcgcct cacaagcgga cgttcgtttt catctggcga
    14281 ttgccgaagc ctcacataac atcgtgctgc tgcaaaccat gcgcggtttc ttcgatgtcc
    14341 tgcaatcctc agtgaagcat agccgtcagc ggatgtatct ggtgccaccg gttttttcac
    14401 aactgaccga acaacatcag gctgtcattg acgccatttt tgctggtgat gccgacgggg
    14461 cgcgtaaagc aatgatggcg caccttagtt ttgttcacac caccatgaaa cgattcgatg
    14521 aagatcaggc tcgccacgca cggattaccc gcctgcccgg tgagcataat gagcattcga
    14581 gggagaaaaa cgcatgatta tttccgcagc cagcgattat cgcgccgcag cgcaacgcat
    14641 tctgccgccg ttcctgttcc actatatgga tggtggtgca tattctgaat acacgctgcg
    14701 ccgcaacgtg gaagatttgt cagaagtggc actgcgccag cgtattctga aaaacatgtc
    14761 cgacttaagc ctggaaacga cgctgtttaa tgagaaattg tcgatgccgg tagcactggc
    14821 accggtgggt ttgtgtggca tgtatgcgcg tcgtggcgaa gttcaggcag ccaaagcggc
    14881 ggacgcgcat ggcattccgt ttactctctc gacggtttcc gtttgcccga ttgaagaagt
    14941 cgcgccagcc atcaagcgcc caatgtggtt ccagctttat gtactgcgcg atcgcggctt
    15001 tatgcgtaac gcgctggagc gagcaaaagc agcgggttgt tcgacgctgg ttttcaccgt
    15061 ggatatgccg acaccgggcg cacgctaccg tgatgcgcat tcaggtatga gcggcccaaa
    15121 tgcggcaatg cgccgctact tgcaggcggt gacgcatccg caatgggcgt gggatgtggg
    15181 cctgaacggt cgtccacatg atttaggtaa tatctcagct tatctcggca aaccgaccgg
    15241 actggaagat tacatcggct ggctggggaa taacttcgat ccgtccatct catggaaaga
    15301 ccttgagtgg atccgcgatt tctgggatgg cccgatggtg atcaaaggga tcctcgatcc
    15361 ggaagatgcg cgcgatgcag tacgttttgg tgctgatgga attgtggttt ctaaccacgg
    15421 tggccgccag ctggacggtg tactctcttc cgctcgtgcg ttgcccgcta ttgcagatgc
    15481 ggtgaaaggt gatatcgcca ttctggcgga tagcggaatt cgtaacgggc ttgatgtcgt
    15541 gcgtatgatt gcgctcggtg ccgacaccgt actgctgggt cgtgctttcc tgtatgcgct
    15601 ggcaacagcg ggccaggcgg gtgtagctaa cctgctaaat ctgatcgaaa aagagatgaa
    15661 agtggcgatg acgctgactg gcgcgaaatc gatcagtgaa attacgcaag attcgctggt
    15721 gcaggggctg ggtaaagagt tgcctacggc actggctccg atggcgaaag ggaatgcggc
    15781 atagtcgttt gcgccccctc accctcaggg agaggggacc gttcggcgct gtatgtactc
    15841 cctcactctg aaacgacacc gcactctttt tttctccctc gcccctccgg ggagggccgg
    15901 ggtgagggga aaaggccgca cggttcacca attttctgct atcctgcccc cgcactaagg
    15961 gggcagtatg ctaaacatcg tactttacga accagaaatt ccgccaaata ctggcaacat
    16021 catccgtctt tgcgctaata ccggctttcg tctgcatatc atcgaaccga tgggatttgc
    16081 ctgggacgat aagcgcctgc gccgcgcggg gctggactat cacgagttta ccgccgttac
    16141 gcgtcatcat gactatcgcg cgttcctcga agcagaaaat ccccagcgcc tgtttgccct
    16201 caccacgaaa ggtacgcctg ctcacagcgc cgtaagctat caggatggcg attatctgat
    16261 gttcggcccg gaaacacgcg gcctgccagc gagcattctt gatgccctgc ccgctgaaca
    16321 aaaaattcgc attccgatgg tgccggacag ccgcagcatg aatctgtcca atgcggtgtc
    16381 ggtagtggtg tatgaagcct ggcggcagct gggatatccg ggagcgttgt tgagagatta
    16441 gttactgtat gccggataga tgattacatc gcatccggca cgatcacagg acattagatc
    16501 ccatccccat actcaaatgt atggttaata ccgttgaaat gctggtccat atccattgat
    16561 ggcttatcgc tgtctggttt accgacaata cgagccggaa cgccagcggc ggttgtatgc
    16621 ggcggcaccg gttgcagcac cacggaacct gcgccaatct tcgcgccgcg accaacttca
    16681 atattgccga ggattttcgc gcccgcgcca atcatcacac cttcacgaat tttcgggtga
    16741 cggtcaccac cagatttacc cgtaccgcca agcgtcacag attgcagaat cgatacgtcg
    16801 ttttcaatta ccgccgtttc acccacaacg atgcccgtgg cgtggtcgag cataatgcca
    16861 cggccaattt ttgctgccgg gtgaatatcg acctggaacg tcacagaaac ctggttttgc
    16921 agaaagattg ccaatgcgcg acgcccctga ttccacaacc agtgaccgat gcgataggcc
    16981 tgcaaggcat gaaaaccctt caggtataac aacggggttg agtatttatc gactgccggg
    17041 tcgcgggtac gcaccgcctg aatatcacag gccgcagagg cgatcatttc cgggtcagcg
    17101 gcgtaggctt cttccaccac ttcacggata gcaatagcag gcataattgg cgatgacagc
    17161 ttgttcgcca gcatgtagct cagtgcacta ccaaggtttt cgtgcttgag tagcgtcgcg
    17221 tggtaaaaac tggccagcat tggctcacag tccgccagcg ttctggcttc ggctttaata
    17281 ttgttccaga caatttccag ttcttcacac gacattgctt actccacacg atgagataat
    17341 gaccggcccg ttctgcgcgg gccgggtcat agcggtaaca aaggttccct ggggttagtg
    17401 gctgctgcgc tcgtccttgc gtgcacgacc taataacgtc aatgctgcct cgcgcgcgtt
    17461 ttttccgcaa tataatactt gataaatttc ctcggttatt ggcatttcta cgccgaagcg
    17521 atgcgccagt tcgcggactt ctttcgtatt gcggtagcct tccaccacct gaccaatctt
    17581 ctcctgcgcg ctttgtacat ccatgccctg accgagcatc atgccaaaac ggcggttacg
    17641 cgactggttg tcggtacagg taagcaccag atcgcccagc cccgccatgc ccataaaggt
    17701 ggcaggatcg gcacccagcg ccgcgccaag acgcgacatt tcagccagcc cacgggtgat
    17761 cagtgccgta cgcgcattcg caccaaaacc gataccgtcg gacatccccg cgccaatggc
    17821 aatgacgttt ttcaccgcac cgccaagctg cacaccgatg aaatccgggt tgctgtaaac
    17881 gcggaaactt ttgccacagt gcagcaattg ttggagatca tcggcaaagg tctggtcggt
    17941 agaggccaac gaaatagccg tcggtaaacc tgccgccagt tctttcgcaa acgttgggcc
    18001 agagataacc gccagcggaa tttgatcgcc taaggcctca cgcgccacgt cctgtaacag
    18061 acgtccggtt tccgcttcca gccctttggt cgcccacacc agacgcgcat caggacgcat
    18121 cagcggttta atctggcgca gcacttcacc aaagacatgg ctgggtacga cgacgagaat
    18181 attacggctg gctgccagcg cagtggcgag atcgctttca agatggagcg tatcgggaaa
    18241 aggcacatcg gggagaaacg cggcgttaca gcggtcgcgt tcaagcgttg cgatatgttc
    18301 agggtcatgg ccccagagga caacctcgtg gccatttctt gccagggtaa tggcaagagc
    18361 ggtgccgtac gagccggcac cgatcacagt cattgaagca ttacgttggt tcatcaggca
    18421 tcctgatgtt cttcagtacc ttcgccagcc tgctgctgca aatagttcat gaacagcgca
    18481 tcgaagttaa tcggcgcaag gttcagttgc gggaatgtac cgcgggatac cacgctggtg
    18541 atgcactcac gagcatacgg gaacagaatg ttcgggcagt atgcgcccag gcaatgtgcc
    18601 atctgggtgc cttcgatacc cgcgatggag aaaataccgc cctgctgaac ttcacacagg
    18661 aacgcggttt cttcgcccag ggaggccgtt acggtaacac gcagtaccac ttcgtatacg
    18721 tcatctgcca gttgggaaga tgccgtatcc agatcaagtt taacttctgg ttgccaatct
    18781 ttctggaaaa cgtgcggcgc gttcggcgct tcgaaagaga tatccttggt ataaatacgt
    18841 tggatctgga aagtcatttc agtgttgttt tgttctgaca tgtgtagaaa acccttaagt
    18901 gttgtcctta aatactgcgc aatgtaatgc caacgttcac acgttatttc agcaggggat
    18961 ccagtccacc acgtgcatcc aatgcataca agtcatcaca gccgccaatg tgctgtgcgt
    19021 caataaaaat ctggggaacc gtggtgcgac cgctgcgttt gatcatctct tcacgcttgg
    19081 cggcattgcc atcgatcggc aactcctgaa aactcacgcc cttgctgctc agcagtgctt
    19141 ttgcacgatg gcaatacggg caggtttctt tggtatagat ttcaacattg gccataattt
    19201 agctcctgtt tttttaccct gaaatttcat gttgcagggc ggctgcaaaa tggagtgtcc
    19261 ccagaagctt ataacaataa gtcgctgggg gcctaattgc agctaacggc ctgcatcatg
    19321 aaagacgaca ggtaaattat ttgccgcgca ccagaggcaa gttttcgcca gcccagccag
    19381 caacgccctc tttcagcacg aatacttgcg caaaaccagc tttcgtcagc gcgtttgcag
    19441 gctcctggca ctgcatgcca gaaccgtcta ccacgataac cggtttgtct ttgtgcttct
    19501 caagctcacc aacattgttg gctttgattt cgctcggcaa cagattgata gaacctgcga
    19561 tatggccttt acggaagtca tcacgctgac gtaaatccac aaccacagcg tcttctttgt
    19621 tgatcagacg cgtagcttca ccacgagtaa tcaccttcac tttcgaggtc aggctcttaa
    19681 acgtagtcac aagaaccgcc accagtaacg cgatccaggc gatactcagt atgggatgac
    19741 ggccaacaaa ttgcataatt tcttgcatgg ggggtaacaa ctcccgacgt agtgattaaa
    19801 aaaaccagga aaggagtata cctgcgcggt gcggcaaata cagccagcgc gttaactgga
    19861 atgcaatttt gcggggcacg acgaaaaaaa agcgcaaatc tggcatcggc ttgccgcgag
    19921 cggtcgtata ttttgatctt cagaggctat tttatcgatt cagctgtagt aaaattacgc
    19981 aaattttgac tcttgagtat gaggttgtcg caatgtcggt ttctaaaaaa cctatggtac
    20041 tggtgattct ggatggctat ggctatcgtg aagaacagca ggataacgcc atttttagtg
    20101 ctaaaacccc ggtaatggat gcactgtggg ccaatcgtcc gcataccctg atcgacgctt
    20161 ccggtctgga agtcggtctg cctgaccgtc agatgggtaa ctccgaagta ggccacgtta
    20221 acctgggtgc cggccgcatc gtgtatcagg acctgactcg tctggacgtt gaaatcaaag
    20281 atcgcgcttt ctttgctaat ccagtgctga ctggcgcggt agataaagcg aaaaacgcag
    20341 gtaaagcagt acacattatg ggtctgctct ccgcaggcgg cgtacacagc cacgaagatc
    20401 acatcatggc gatggtagaa ctggcagctg aacgcggcgc agaaaaaatc tacctgcacg
    20461 cattccttga cggtcgcgac accccgccgc gcagtgctga atcctcgctg aaaaaatttg
    20521 aagagaaatt tgctgcgctg ggtaaaggcc gcgtagcgtc catcattggt cgttactacg
    20581 cgatggaccg cgataaccgt tgggatcgcg ttgaaaaagc ctatgacctg ctgactctgg
    20641 cgcagggtga gtttcaggcc gatactgccg ttgctggttt gcaggcagct tatgctcgcg
    20701 acgaaaacga cgagttcgtg aaagcgaccg ttattcgtgc agaaggtcag ccagatgcgg
    20761 caatggaaga cggcgatgcg ctgattttca tgaacttccg tgctgaccgc gcgcgtgaaa
    20821 tcactcgtgc attcgtgaac gctgatttcg atggcttcgc gcgtaagaaa gtggttaacg
    20881 tcgatttcgt gatgctgacc gaatacgccg ctgacatcaa aactgcggtt gcttacccac
    20941 ccgcttccct ggttaacacc ttcggcgagt ggatggcgaa aaacgacaaa actcagttgc
    21001 gtatttccga aaccgaaaaa tatgcccacg ttactttctt cttcaacggt ggcgtagaag
    21061 agtcgttcaa aggcgaagat cgcattctga tcaactcacc gaaagtggct acctacgatc
    21121 tgcaaccgga aatgagctcc gcagagctga ccgaaaaact ggttgcggcc atcaagagcg
    21181 gcaaatacga caccattatc tgtaactatc cgaacggcga catggtaggt cacaccgggg
    21241 tgatggaagc ggcggttaaa gcggttgaag cactggatca ctgcgtggaa gaagtcgcga
    21301 aagcggttga gtccgtgggt ggacaactgc tgatcaccgc tgaccacggt aacgctgaac
    21361 agatgcgcga tccggcaacg ggtcaggcac acacggcaca caccaacctg ccagttccgc
    21421 tgatttacgt tggtgataag aacgtgaaag cggttgcagg cggcaaactt tctgacatcg
    21481 cgccgaccat gttgtcgctg atgggtatgg aaatcccgca agagatgact ggtaagccgc
    21541 tgttcatcgt ggaataatcc ctccccatga ggggaaaggc gattaatacc atgacacggg
    21601 ccgtgaaacc gcgcaggttt gcaatcaggc ccatcatcta cgccagcgtt cttagcgctg
    21661 gcgtattgtt gtgcgccttt tccgcccacg cggatgagcg tgaccaactc aaatctattc
    21721 aggccgatat cgccacaaaa gagcgcgcgg tacgccaaaa gcaacaacaa cgcgcaagcc
    21781 tgctcgcaca attgaaaaag caggaagaag cgatctctga agccacccgt aagctgcgcg
    21841 aaacgcaaaa cacgctcaat caactcaata aacagattga tgagatgaac gcgtcgattg
    21901 ccaaactgga gcagcaaaaa gccgcccagg agcgcagcct cgccgcacaa ctggatgccg
    21961 cattccgtca gggcgagcat accggtattc agctaattct cagcggtgaa gaaagccagc
    22021 gtggacagcg tttacaggct tatttcggct atctcaacca ggcgcgacaa gaaaccattg
    22081 cccagttgaa gcaaacgcgt gaagaagtcg ccatgcagcg tgctgaactg gaagagaaac
    22141 aaagcgagca acaaacgctg ttatatgaac agcgcgccca acaggcgaaa ctgactcagg
    22201 ctctgaacga gcgtaaaaag acgctggcgg ggctggaatc ttccatccag caaggtcagc
    22261 agcagctaag tgaactgcgt gccaacgaat cccgtctgcg taacagtatt gcccgtgcgg
    22321 aagccgcggc gaaagcgcgt gctgaacgag aagcgcgtga ggctcaggca gttcgcgaca
    22381 ggcagaaaga agcgacgcgc aaaggcacca cctacaaacc gaccgaaagc gaaaaatcgc
    22441 tgatgtcccg aactggtggc ctgggtgcgc cgcgtggtca ggcattctgg ccagttcgtg
    22501 gccctacgct gcatcgctat ggcgagcagc tacagggtga attacgctgg aaaggaatgg
    22561 ttatcggtgc ctctgaaggt actgaagtta aagcaattgc cgacggtcgg gtgattctgg
    22621 ctgactggct gcaaggctac ggtctggtgg tggtggttga gcacggtaaa ggcgatatga
    22681 gtctttacgg ctataatcaa agcgcgctgg tgagcgttgg ttcgcaggtt cgtgcgggcc
    22741 agccaattgc actggtgggc agcagtggcg gtcagggcag accttcactc tatttcgaaa
    22801 ttcgccgcca gggtcaggcc gtcaatccac agccgtggtt gggaagataa gttttgtttc
    22861 catttcgtcg taacgttctt gcatttgccg ctctgttggc gctctcctcc cctgtaattg
    22921 ctggcaaact tgccatcgtc attgatgatt ttgggtatcg cccgaacaac gaaaaccagg
    22981 tgctggcgat gccttccgct atctccgtcg ctgtattacc cgattcaccg cacgccagag
    23041 agatggcgac caaagcgcat aacagcggtc acgaagtgtt gattcatctc ccgatggcac
    23101 cgttgagtaa acagccgctg gagaaaaata cgctgcgccc ggagatgagc agcgacgaaa
    23161 ttgagcgcat tattcgtagt gcggtcaata acgtgcccta tgccgtgggg atcaataacc
    23221 acatgggcag caagatgacc tctaacctgt ttggtatgca gaaagtgatg caggcgctgg
    23281 agcgttataa tctttacttc ctcgacagcg taaccattgg taatacccag gcgatgcgcg
    23341 ccgcgcaagg cactggcgtg aaggtgatca aacggaaggt gttcctcgac gattcacaga
    23401 acgaagcgga catccgtgtg caattcaatc gcgcaattga cctagcgcgt cgcaacggat
    23461 caaccattgc cattgggcat cctcaccctt caacggtacg cgtgttgcaa cagatggttt
    23521 ataacctgcc gccagacatt acgctggtga aagccagcag cttgctgaat gaaccgcagg
    23581 ttgatacgtc tacaccgccg aaaaacactg tgcccgacac accgcgtaat ccgttccgtg
    23641 gcgtgaagct gtgcaaaccg aagaaaccga tagaacctgt ttatgctaat cgcttctttg
    23701 aagtattaag cgaaagcatc agccagagca cgctgatcgt ttacttccag catcagtggc
    23761 aaggctgggg caaacagcct gaagcggcga agcttaacgc tagcgtaaat taaaggcgct
    23821 acgcgtaatg cgatggtgcg accgcgtttt gtcgcgccat tgccataagc ggaaagacca
    23881 cagtaacgcc tgataaccca cctttacgct gcgcacattg gtaatcaggc gcttataca
//
LOCUS       DAESHI010000059        23728 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.059, whole genome shotgun sequence.
ACCESSION   DAESHI010000059 DAESHI010000000
VERSION     DAESHI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23728
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.059"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..800)
                     /locus_tag="KW011_002424"
     CDS             complement(<1..800)
                     /locus_tag="KW011_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBH7660734.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKLDLYGKVDARHTF
                     SDNPGDDGDETIIELGFKGETQITDQLTGYGQALTKTKASDTEGSDNTYVKLAFAGLK
                     FGEMGSFDYGRNYGVIYDVEAWTDMLPVFGGDSYTWTDNFMAGRANGVATYRNSDFFG
                     LVEGLNFALQYQGNNEGSNAGEDQEGTKNGHEDVRFQNGDGFGLSTSYDFDFGLSLGA
                     AYSNSDRTNSQVALGGYHYNEYSKFAGGDTAEAWTFGAKYDANNVYLA"
     gene            1367..1732
                     /locus_tag="KW011_002425"
     CDS             1367..1732
                     /locus_tag="KW011_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBH7660735.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
     gene            1772..2428
                     /locus_tag="KW011_002426"
     CDS             1772..2428
                     /locus_tag="KW011_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBH7660736.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            2495..3913
                     /gene="dcm"
                     /locus_tag="KW011_002427"
     CDS             2495..3913
                     /gene="dcm"
                     /locus_tag="KW011_002427"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HBH7660737.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            3894..4364
                     /gene="vsr"
                     /locus_tag="KW011_002428"
     CDS             3894..4364
                     /gene="vsr"
                     /locus_tag="KW011_002428"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HBH7660738.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(4353..5274)
                     /gene="yedA"
                     /locus_tag="KW011_002429"
                     /pseudo
     CDS             complement(4353..5274)
                     /gene="yedA"
                     /locus_tag="KW011_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            5447..6364
                     /locus_tag="KW011_002430"
     CDS             5447..6364
                     /locus_tag="KW011_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HBH7660739.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            6443..6625
                     /locus_tag="KW011_002431"
     CDS             6443..6625
                     /locus_tag="KW011_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HBH7660740.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            6796..8490
                     /gene="yedQ"
                     /locus_tag="KW011_002432"
     CDS             6796..8490
                     /gene="yedQ"
                     /locus_tag="KW011_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HBH7660741.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(8487..9302)
                     /locus_tag="KW011_002433"
     CDS             complement(8487..9302)
                     /locus_tag="KW011_002433"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HBH7660742.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(9600..9827)
                     /gene="yodD"
                     /locus_tag="KW011_002434"
     CDS             complement(9600..9827)
                     /gene="yodD"
                     /locus_tag="KW011_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HBH7660743.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            9990..10178
                     /gene="dsrB"
                     /locus_tag="KW011_002435"
     CDS             9990..10178
                     /gene="dsrB"
                     /locus_tag="KW011_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HBH7660744.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(10222..10845)
                     /gene="rcsA"
                     /locus_tag="KW011_002436"
     CDS             complement(10222..10845)
                     /gene="rcsA"
                     /locus_tag="KW011_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HBH7660745.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(11130..11915)
                     /gene="fliR"
                     /locus_tag="KW011_002437"
     CDS             complement(11130..11915)
                     /gene="fliR"
                     /locus_tag="KW011_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HBH7660746.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            complement(11924..12193)
                     /gene="fliQ"
                     /locus_tag="KW011_002438"
     CDS             complement(11924..12193)
                     /gene="fliQ"
                     /locus_tag="KW011_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBH7660747.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(12203..12940)
                     /gene="fliP"
                     /locus_tag="KW011_002439"
     CDS             complement(12203..12940)
                     /gene="fliP"
                     /locus_tag="KW011_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBH7660748.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            complement(12940..13305)
                     /gene="fliO"
                     /locus_tag="KW011_002440"
     CDS             complement(12940..13305)
                     /gene="fliO"
                     /locus_tag="KW011_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HBH7660749.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(13308..13721)
                     /gene="fliN"
                     /locus_tag="KW011_002441"
     CDS             complement(13308..13721)
                     /gene="fliN"
                     /locus_tag="KW011_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HBH7660750.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            complement(13718..14722)
                     /gene="fliM"
                     /locus_tag="KW011_002442"
     CDS             complement(13718..14722)
                     /gene="fliM"
                     /locus_tag="KW011_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HBH7660751.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(14727..15191)
                     /gene="fliL"
                     /locus_tag="KW011_002443"
     CDS             complement(14727..15191)
                     /gene="fliL"
                     /locus_tag="KW011_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HBH7660752.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            complement(15296..16423)
                     /gene="fliK"
                     /locus_tag="KW011_002444"
     CDS             complement(15296..16423)
                     /gene="fliK"
                     /locus_tag="KW011_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HBH7660753.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(16420..16863)
                     /gene="fliJ"
                     /locus_tag="KW011_002445"
     CDS             complement(16420..16863)
                     /gene="fliJ"
                     /locus_tag="KW011_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HBH7660754.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(16882..18255)
                     /gene="fliI"
                     /locus_tag="KW011_002446"
     CDS             complement(16882..18255)
                     /gene="fliI"
                     /locus_tag="KW011_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HBH7660755.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            complement(18255..18941)
                     /gene="fliH"
                     /locus_tag="KW011_002447"
     CDS             complement(18255..18941)
                     /gene="fliH"
                     /locus_tag="KW011_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBH7660756.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            complement(18934..19929)
                     /gene="fliG"
                     /locus_tag="KW011_002448"
     CDS             complement(18934..19929)
                     /gene="fliG"
                     /locus_tag="KW011_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBH7660757.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            complement(19922..21580)
                     /gene="fliF"
                     /locus_tag="KW011_002449"
     CDS             complement(19922..21580)
                     /gene="fliF"
                     /locus_tag="KW011_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBH7660758.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            21796..22110
                     /gene="fliE"
                     /locus_tag="KW011_002450"
     CDS             21796..22110
                     /gene="fliE"
                     /locus_tag="KW011_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBH7660759.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            22444..22776
                     /gene="emrE"
                     /locus_tag="KW011_002451"
     CDS             22444..22776
                     /gene="emrE"
                     /locus_tag="KW011_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HBH7660760.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            22945..23496
                     /gene="ybcL"
                     /locus_tag="KW011_002452"
     CDS             22945..23496
                     /gene="ybcL"
                     /locus_tag="KW011_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HBH7660761.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            23506..>23728
                     /locus_tag="KW011_002453"
     CDS             23506..>23728
                     /locus_tag="KW011_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH7660762.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYESYIANYPEAG"
BASE COUNT         5759 a         5946 c         6139 g         5884 t
ORIGIN      
        1 attgcaagat aaacattgtt ggcgtcgtat tttgcaccaa atgtccatgc ttcagctgta
       61 tcaccaccag caaatttgct gtattcattg tagtgatacc cgccaagagc tacttgagaa
      121 ttggtacggt cagagtttga gtaagcggca cctaagctca aaccgaagtc aaagtcatat
      181 gaagtggaaa gaccaaaacc atcaccgttc tggaagcgaa cgtcttcgtg accattttta
      241 gtaccttctt gatcttctcc tgcattgctg ccttcgttgt taccttgata ctgcaatgca
      301 aagttcagac cttccaccag accgaagaaa tcactattgc ggtaagtagc taccccattt
      361 gcacggcctg ccatgaagtt atcagtccag gtataggagt cgccgccgaa tacaggaagc
      421 atatctgtcc aggcttcaac gtcgtaaatc acaccatagt tacgaccata gtcaaaggag
      481 cccatctcgc cgaacttcag acctgcaaaa gcaagtttga cataagtatt atcagaacct
      541 tcagtgtcgc tagcctttgt tttggtcagt gcttgaccat aaccagttaa ctgatctgta
      601 atttgggtct cacctttgaa gcctaactcg atgatagttt catcgccgtc atcgccaggg
      661 ttatcggaaa aagtatgacg agcatcaact tttccgtaca gatccagttt attgccattt
      721 ttattataaa tttcagccgc atttgctgcg ccagcaacta ataacgctgg gaccagcatt
      781 gccagaactt ttcttttcat tatgtattcc cttgtgatta taatcttcat gaatatatca
      841 ataagtgccg ttatccaaaa aaaaagcaca tttggatact attctatgaa gttcatttta
      901 ttttaaagat tgcgtgtaac aaatgtattt aaaagatttc aaaatgtttc taatctattt
      961 aatataattc agaattatga attctaaata taaacatcca tgaaaattat aaatgtatat
     1021 caatatgtta tgagttgaat ggaaagatag ggagtatcaa tgattgttgc gccaaacggc
     1081 aggcgtgatc acgcgcacat taatgaaggt ataaaaaaag ccggcatcat gccggcccaa
     1141 aaaatataaa caagacgata aaaggctaat aacggggtta tcatgacaca gaaatcaaaa
     1201 aaggtataat tgtattcagt gctaatatat tgtttgggtg aattaataaa actattacca
     1261 aggatataaa taaatcttta ttgctgtttt tccagtaatt attgccgttg cgcaaaattt
     1321 atctatttgt tcaaaaaatg attgagtctt gactggctca tccaatgtgg aaaaatgtga
     1381 cttttatcac ataatagtac taagtctgaa ttttccgggt tatctcaaaa tggaatacgg
     1441 ttcgacaaag atggaagaga gactctctcg cagccctggc ggcaaattgg ctttgtgggc
     1501 tttctataca tggtgtggct atttcgtctg ggcaatggcg cgttacatct gggtgatgag
     1561 ccggatccct gatgccccgg tgtcgggatt tgaaagcgat ttaggctcta ccgcgggaaa
     1621 gtggttaggg gcactggtcg gatttttatt tatggctctg gtcggggcat tgttaggcag
     1681 cattgcctgg tacacccgac ctcgtcccgc acgtagcaga cgctatgaat aaaaactgct
     1741 aaggtggcga ctctctggca acggagcgcg tatggactta caacactggc aggcacagtt
     1801 cgaaaactgg ttgaaaaacc atcatcagca ccaggacgcg gcacagaagc tggcggcaga
     1861 tgacgatgtc gatatgctgg tgattttaac cgcctgttat tttcacgata ttgtgagcct
     1921 ggcgaaaaat catccgcaaa ggcagcgttc ttcaatcctg gcggcagaag agacgcgtcg
     1981 cctgctccgt gaagagttcg agcaatttcc ggcggagaaa atcgaggccg tttgtcatgc
     2041 cattgccgct cacagtttca gcgcgcaaat cgccccctta acaacggaag ctaaaatcgt
     2101 ccaggatgcg gatcggctgg aagccctggg ggcgattggc ctggcgcgcg tgtttgccgt
     2161 ttcaggggca ttgggcgtgg cgctgtttga tggtgaagat ccgttcgcac agcatcgccc
     2221 gcttgatgat aaacgctacg cgctggatca tttccagact aagttgctga agctaccgca
     2281 aaccatgcaa accgcaagag gcaagcagct ggcgcagcac aacgcgcatt ttttagtcga
     2341 gtttatggcg aagctcagtg ccgaactggc gggggagaat gaaggtgtcg atcacaaggt
     2401 gatagatgcg ttttcacccg cgggataagc gcgtggccct aaatggctgt aattatgtta
     2461 acctgtcggc catctcagat ggccggttaa atctatgcag gaaaatatat cagtaaccga
     2521 ttcatacagc accgggaatg ccgcacaggc aatgctggag aaactgctgc aaatttatga
     2581 tgttaaaacg ctggtggcgc agcttaatgg tgtgggtgag aatcactgga gcgcggcaat
     2641 tttaaaacgt gcgctggcga atgactcggc atggcaccgt ttaagtgaga aagagttcgc
     2701 ccatctgcaa acgttgttac ccaaaccatc ggcacatcat ccgcattatg cgtttcgctt
     2761 tatcgatcta tttgccggaa ttggcggcat ccgtcgcggt tttgaatcga ttggcggaca
     2821 atgcgtgttt accagcgaat ggaacaaaca tgcggtacgc acttataaag ccaaccatta
     2881 ttgcgatccg gcgacgcatc attttaatga agatatccgc gacatcaccc tcagccataa
     2941 agaaggcgtg agtgatgagg cggcggcgga acatattcgt caacacattc ctgaacacga
     3001 tgttttactg gccggtttcc cttgtcagcc attttcgctg gctggcgtat cgaaaaagaa
     3061 ctcgctcggg cgggcgcacg gttttgcctg cgatacccag ggcacgctgt tttttgatgt
     3121 ggtacgcatt atcgacgcgc gtcgtccggc gatgtttgtg ctcgaaaacg tcaaaaacct
     3181 gaaaagtcac gaccagggta aaacgttccg catcatcatg cagacgctgg acgaactggg
     3241 ctatgacgtg gctgatgcag aagataacgg gccggacgat ccgaaaatca tcgatggtaa
     3301 acattttctg ccgcagcacc gtgaacgcat cgtgctggtg ggttttcgtc gcgatcttaa
     3361 tctgaaagcc gattttactc tgcgtgatat cagcgaatgt ttccctgcac agcgagtgac
     3421 gctggcgcag ctgttggacc cgatggtcga ggcgaaatat atcctgacgc cggtgctgtg
     3481 gaagtacctc tatcgatatg cgaaaaaaca tcaggcgcgc ggtaacggct tcggttatgg
     3541 aatggtttat ccgaacaatc cgcaaagcgt cacgcgtacg ctgtctgcgc gttattacaa
     3601 agatggcgcg gaaattttaa tcgatcgcgg ctgggatatg gccacgggtg agaaagattt
     3661 tgacgatccg ctgaatcagc aacatcgtcc acgtcggtta acgcctcggg aatgcgcgcg
     3721 cttaatgggt tttgaagcgc cgggagaagc gaaattccgc attccggttt cggacactca
     3781 ggcctatcgc cagttcggta actcggtggt cgtgccggtc tttgccgcgg tggcaaaact
     3841 gcttgagcca aaaatcaaac aggcggtggc gttgcgtcag caagaggcac aacatggccg
     3901 acgttcacga taaggccact cgcagcaaaa atatgcgcgc gattgccacg cgtgatacgg
     3961 cgattgagaa gcgcctcgcc agtctgttaa ccgggcaggg cctggcgttt cgcgttcagg
     4021 acgccagtct gcccggaagc ccggattttg tcgttgatga atatcgctgc gtgatattta
     4081 cccatggctg cttctggcat catcatcact gctatctgtt taaagtgcct gcgactcgaa
     4141 ccgagttctg gctggagaag ataggtaaaa atgttgaacg cgatcgccgc gatatcagtc
     4201 gtttgcagga actcggctgg cgcgtactga ttgtctggga gtgtgcatta cgtgggcgcg
     4261 agaagctgac ggatgaagcg cttaccgagc gtctggaaga gtggatctgc ggcgaaggtg
     4321 ccagcgcgca gatcgacacg caggggattc atttactcgc ttgatgcgtc ctgaataact
     4381 ggcgcaacta cgggttttgc cgggaagaga tattttccca acgtgaccag taccaccgcg
     4441 aagacaatta cgccgagcgc cagccattca atcttcgaca gcgtctctcc gcccagtccc
     4501 gtacccagca agaccgcgac caccgggtta acgtaggcgt agctggtggc gagagccgga
     4561 ctgacattac ggattaaata catataagcg ttgatggcga taatcgaacc aaacagcgcc
     4621 agatagccga ccgcaaggaa gcctgaaagg gaagggagcg ccgtcagttt ttcacctgca
     4681 atcatcgacg cgatcattaa caccacgcct gccgccagca tctcaatcgc acccgccatc
     4741 atccctacag gtaaggtaat gcgcgagcca taaaccgagc caaacgccca gctaatcgag
     4801 ccgattaaaa tcagaatcgc gccccacgga ttgccgctta aatttccacc gctattgagc
     4861 atgatgattc cggcaagccc aatggcgata cccacccatt ccagtttgcg cgttttaatg
     4921 ccaaacaggc ggctgaagca ccagggtaaa gaggggcacg gttgcaacca caacggcggc
     4981 gatgccggaa ggaacatttt gatgctcggc aaccgtcacc atgccattac cgacagccag
     5041 caataacagg ccaatcagcg cggcattgag cagcggacgt agcgggggga gcttgtgtcc
     5101 gcgcagtagc aaaaatgcca tcaataaaat accggctgcc aggaatcgaa cgcctgccat
     5161 cattaacgga ggccagcttt ccacgccaat ccgaatgaca aaataggttg agccccaaat
     5221 gatatacaac gcaaacagcg cgccaaaaag cggtaacaac tggcggaaac gcataatccc
     5281 tcacggtgga aataaaaagg tggttcatag taaacgtgaa aatcattctg ctggcgagag
     5341 atataattgc acttgattgt aaaaaaaatg ttgacctgtg aagcaagtat cagagctgcc
     5401 gtttttgctt catacttaca cccttcaaca ataaaaatga gagggaatgc ttttggccgg
     5461 gagtagttta ctgacgttgc tcgatgatat cgccacacta ctggacgata tctccgtgat
     5521 gggcaaactg gcggcgaaga aaaccgccgg tgtattaggg gatgacttat cgctcaatgc
     5581 gcaacaagtt tcaggcgtgc gggccaaccg ggaacttccc gtggtctggg gcgtggcgaa
     5641 aggatcgctg attaataaag tgattctggt gccgctggcg ctgatcatca gtgcgtttat
     5701 cccgtgggcg attacgcctc tgttgatgat tggtggcgcg tttctctgct ttgaaggagt
     5761 agagaaagtg ctgcatatgc tggaggcgcg taaacataaa gaagatccgg cgcagagcca
     5821 gcagcgtctg gagaagctgg cggcgcagga tccgctgaag tttgaaaagg acaaaataaa
     5881 aggggcgatt cgtaccgatt ttatattgtc tgccgaaatc gtcgccatca cgctggggat
     5941 tgtggccgaa gcgccgttgc ttaatcaggt gctggtgctt tcaggcatcg cgctggtagt
     6001 gaccgtgggc gtctacggtc tggtaggggt tatcgttaag attgatgacc tggggtattg
     6061 gctggcggaa aaatccagcg cgctgatgca ggcattaggt aaagggttat tgatcattgc
     6121 gccctggttg atgaaggcgt tatcgattgt cggcacgctg gcgatgttcc tcgtcggcgg
     6181 cggtattgtg gtacatggta ttgcgccgct gcatcacgcc attgaacatt tcgccgggca
     6241 gcaaagtgca gtggtggcga tgatattacc gactgtttta aatctgattc ttggatttat
     6301 catcggcggc atcgtggtgc tgggagtgaa agccgtagcg aaaatgcgcg gtcaggcaca
     6361 ttaatactcg tacaggctat tatgcaaaat tcgccatctt caactaaggt gaacaagttt
     6421 cactcgaaaa aaggaggcga gtatgagctt tatggttagt gaggaagtta cggttaaaga
     6481 gggcggcccg cggatgattg tcactggata ctccagcggt atggttgagt gtcgttggta
     6541 tgacggttac ggggtcaagc gggaagcttt tcatgaaacc gagcttgttc cgggggaggg
     6601 gagtcgttct gcggaagaag tttgaaaggg tatcattaac accaggccgg atcacttccg
     6661 gcctggtgtg ccaatgcctg caatgaatga tagtcgcgtc ttatcatgcc accattataa
     6721 aattatcgct gttttgcggt acgctatcgc caggttatgc cagaatcata aaaaagcagg
     6781 ttgggagtcg tcagggtgca gcacgagaca aaaatggaaa accagagctg gttgaaaaaa
     6841 ctcgcacgcc gcctggggcc tggtcatgtc gttaatctct gctttatcgt ggtattgctt
     6901 ttttccacct tgctgacctg gcgtgaagtg gtagtgctgg aagatgccta tatctccagc
     6961 cagcgtaatc atctggaaaa cgtagccaac gcgctcgata agcatttgca gtataacgtc
     7021 gacaaactga tctttttgcg taatggcatg cgcgaagctc tcgtagcgcc actggatttc
     7081 acttcactgc gtaatgctgt aaccgagttc gaacagcatc gcgacgagca cgcctggcaa
     7141 attgaactca accgacgacg caccctgtca gtcaatggcg tatcggatgc attagtcagc
     7201 gaggggaatc tcctgtctcg cgaaaatgaa agcctcgaca atgaaattac cgctgcactg
     7261 gaagttggtt acttgctgcg actggcgcac aacacctcgt cgatggttga acaggcgatg
     7321 tatgtctcgc gtgccggatt ttacgtttcg acgcagccga ccttgtttac gcgcaatgta
     7381 ccaacgcgtt attacggcta tgtcacccaa ccctggttta tcggccattc gcaacgagaa
     7441 aatcgtcacc gcgcggtacg ctggtttact tcgcaaccgg aacacgccag caatactgaa
     7501 ccgcaggtta ccgtcagtgt tccggtagac agtaataact actggtatgg cgtgctgggg
     7561 atgagtattc ccgtgcgtac catgcagcaa tttttaagaa acgccatcga taaaaacctc
     7621 gatggtgagt atcagctcta tgacagtaag ctgagatttt tgacctcttc caatcctgac
     7681 catccaacag ggaatatttt tgatcctcgt gaactggcct tgctggcgca ggcgatggaa
     7741 catgacacgc ggggcggcat tcgtatggac agtcgctatg ttagctggga acgtctggac
     7801 catttcgacg gtgtgctggt gcgcgtccat acgctaagcg aaggcgtgcg cggcgatttc
     7861 ggcagtatca gcattgcatt aaccctgctg tgggcgctct ttaccaccat gttactcatc
     7921 tcctggtatg tgattcgccg gatggttagc aacatgtatg ttctgcaaag ctcgttgcag
     7981 tggcaggcgt ggcacgacac cttaacccgt ttatataatc gtggcgcact gtttgaaaaa
     8041 gcccgtccgc tcgcgaaatt gtgtcagacg caccaacatc ctttttctgt cattcaggtc
     8101 gaccttgacc attttaaagc gattaatgac cgctttggtc atcaggcggg cgaccgtgtt
     8161 ctttctcatg ctgccggatt aattagcagt tccttgcgtg cgcaggacgt tgccgggcgg
     8221 gtcggtggtg aggagttttg tgtgattctg ccaggcgcga gtctgacgga ggctgcggaa
     8281 gtcgcagaac gtattcgcct gaagttaaat gaaaaagaga tgttgattgc taagagtacg
     8341 acgatacgca tcagtgcctc gttgggggta agtagcagcg aggaaaccgg tgattatgat
     8401 tttgaacaac tccagtcact ggctgaccgt cggctttatc tcgctaaaca ggccgggcgt
     8461 aatcgggtat gcgcgagcga taacgcttaa cgagcggaga aaaaatggtc cagcccttca
     8521 cgccagcctt ccggtccttc acgctgcgtt cgccagacgc gggtcggatc ctcatcatgc
     8581 agatgcaccc cttcacggtt tagccctttc acaatcaccg cgtaatccat tacctccagt
     8641 aagggcgcat cgtttggccc atcgcccagg ccaagtgtgg ttgggcgttt gcctgataat
     8701 tgttgatagg tcgcgataat ccagttggcg gcctgatctt ttccggcaga ggcatccagg
     8761 acgtgccaga agcgcgcacc ctgcataaac tgtaagccca gttcgttcag acgagcggta
     8821 aattgtgcca tacgctcgtc actgtcgcgc cagattagcg ttaccgacgc ctcatgcagc
     8881 tgcgtcagcg ccgcctggct acggcttaat cccgtccatt cggcgatggt tgcatcgtcg
     8941 acatcatcga aagtcgtgaa tttaaaatgt tctttctcgc gtagcgtatt taaaacctgg
     9001 ctgatttcgc catggctaat acctgagatg atgcgtggaa aaccgtctat atcctgccat
     9061 tgttcagcaa gctggatcac tgcgccattt tcggcaatca gtggtaaccc ttgtagcccc
     9121 aacgtttttt gcaagtacag catttccgct gaagttttac tgctacagag aatgacggga
     9181 acattcgctt cgcgtaaacg gctgagccag agggcagtcg gttgccagtc ataactatga
     9241 ctgtccagca gggtgccatc aagatcgcta aaaaccagta gtggttgttg aattgaaaac
     9301 atggtcgtgc agttctcccc ttaccaggat gattctcaaa acagcacaaa taatcaaaga
     9361 tgattttttc gggagcgatc ccgttgacgc cagagccacc aaggggtgac gcacgtcaca
     9421 tttctattca taagtagcgt taattattca tatcgcgaat attttcttgt cagcgaaaaa
     9481 aattgcggat aaggtgatga acacatcaga tttcctggtg taacgaattt tttaagtgct
     9541 tcttgcttaa gcaagtttca tcccgacccc ctcagggtcg ggattttttt attgtgcatt
     9601 caacgattca cttcagagac gctgaagtca tgaatatcca gttcgaaggc atccgccaat
     9661 tcacgccatg tatgatattc acgtccatcg acgctaacgt gttcagaatc gttctggctg
     9721 cgatgaactt cgctttcgct aatttcattg atcgccgcca gcagggcatc gacatcgacg
     9781 ctgacctcac gttttgcggt atcgctgtac tcttttgcgg ttttcatcat ctttcctcgc
     9841 aaccgttact ctgttgttgc cgccagtcgt taagtccgcc tggcatctct taagtataga
     9901 ccgtcgagaa aatcagcact cgcgcgcgcc tggagctgcc agaaaaacga aattgttcta
     9961 cactggcaca aagccacagg aggaaaacga tgaaggtgaa tgatcgggta acagtcaaaa
    10021 cggatggcgg tccgcgtcgt cctggcgtgg tactggcagt tgaggagttt agtgaaggca
    10081 caatgtacct ggtttcgctg gaagactacc cgctcggcat ctggttcttt aatgaagcag
    10141 ggcatcagga cggtatcttt gtggagaaag cagagtaatc ttattcagcc tgactggtgg
    10201 gaaaccacca gtcagaatgt gttagcgcat gttgacaaaa ataccattag tcacattatc
    10261 cgtcagtcgg acgacatggt agataacctg tttattatgt gttttgatct tacgtttaat
    10321 attaccttta tgcgatgaaa cggtcttggc tttgatattc atttggtcag agatttgaat
    10381 ggttccctga cctgccatcc acattcgcaa catactcgat tcggttcggc tcaatgataa
    10441 cgtcggcata tttaaaaaag aggttatcgt tgtctctttt ttcagaatat cgccaaggat
    10501 atcgtcgaga gattccggtt taatcgattt agaacttatc aataaatttt ttctgaccaa
    10561 tagatattca tcaaaatgaa cattggcaat tgccataaaa acgataaata acgtattggg
    10621 atgttgatta atgatgtgct tgatgtgctg actgttagaa gcatcatgga tgaaacagtc
    10681 ctcattaata aacaccactg aagggcgctg tgaatcacaa gctatggcaa ggtcatcaac
    10741 ggtttcaatg tcgttgattt ctcttttttt aacccctcta ctcaacagat acccggttaa
    10801 acctagtcgg gtgtaactac ataaatccat aataatcgtt gacatggcat accctcactc
    10861 aatgcgtaac gataattccc cttacctgaa tatttcatca tgactaaacg gaacaacatg
    10921 ggtcacctaa tgcgccactc tcgcgatttt tcaggcggac ttactatccc gtaaagtgtt
    10981 gtataatttg cctggaattg tcttaaagta aatgttgcga tatgtgagtg agcttaaaac
    11041 aaatatttcg ccgcaggagt atcctggaag atgttcgtag gtattatttt tcggataatc
    11101 cttaggataa catgataaac gttacggaat tatattaatg gcaattcact aataatatca
    11161 gccagcaaat taaacatctc actgaataaa tgttcgcaaa aaggtgcaat taacggcatt
    11221 aatgccgcca ttaaagagat accgacagtc agagttaatg gaaatccaat aacaaaaatg
    11281 gataattgcg gggccatacg attcagtaaa cctaatgcca gattcagggt cagcagcaga
    11341 gtaatgagcg gtaacgccag catcagcccg ttaaggaaaa tcaaactccc cgctttggtg
    11401 agtgccagaa acgcattgct gttcaacggt tcgccaccaa ttggcagggt gtgaaaggta
    11461 tcgaccagca gtgaaatcaa ccataaatga ccgttaaatg tcaggaacag cagtaacgcc
    11521 agcatatcca tgatacgcgc taaaacgggc atattgagat ggctggccgg atcgacaaac
    11581 gtcgcaaatg acagccccat ttgcagaccg ataatttcgc cagcggttcg cacagcggca
    11641 aaggcaaatt gcatggtaaa accaagcgca atgccgatca ggatctgctg cacggccagc
    11701 cacagagcaa agaacgaaaa aacaggaaca tcgttggcag gtaatgatgg ggcaatgacg
    11761 aacgtgatca tcattgccag ccccagtttt acccgtttcg gcacgctgcg ttcgctcaga
    11821 attggtgcgg tagaaatgag cgccagcacg cgcagtaacg gccagaagta caggcttaac
    11881 caggaaagcc attgatcgct tgtcacctgc atcattgtac ggcctacccg atgatatacg
    11941 gcaggttagt gaacaaggtg cggacgtaat ccagcaacag attgagcatc cacggtccgg
    12001 caataataat ggcaataaat acggcgatga tttttggaat aaacgacagc gtcatttcgt
    12061 tgatctgcgt ggcggcctgc aaaatactga tgataaggcc cgtgaccagc gccaccagca
    12121 acagcggggc agccagtgcc agcgcgactt tcatcgcttc agtccccatc atcatgaccg
    12181 attcaggtgt cattttgcct ctctagctgt aaaagctctg cgccagcgaa ccgaccagca
    12241 attgccagcc atccaccagt acaaacagca tcagtttaaa gggcagagca atggtggcgg
    12301 gggggaccat catcatcccc aatgccatca acacgctggc aatcaccagg tcgataatca
    12361 aaaaaggaat gaaaatcgtg aagcctatct gaaatgcggt tttcaattcg ctggtcacgt
    12421 aggccgggag caaaatgcgc atcggcacgg cttcaggtcc ctgcaacggg ccggtattcg
    12481 ctagtctggc aaacaaccct aaatctgcct cacgggtctg acgcagcata aactcacgta
    12541 gcggctgcgc ccctttttcc agcgcctcct gcattgatat tttctcttcg ctgaatggct
    12601 ggtacgcatc gacataaatt ttgtcgatca ccgatgacat aataaaaaag gtcaaaaata
    12661 gtgccagccc cagcaatacc tggttaggtg gcgcggaggg cgttcccagc gcgttacgca
    12721 ataaaccaaa aacaatgatg atgcgggtga aactggtcat catcagtaaa attgccggaa
    12781 taaacgtcaa cgaggtgatg aacaccagcg tctgcaccgg gagcgaccag ctttgtccac
    12841 cgccaggcag cggctggctg gtgatacccg gcagttgcgc gaaggcgagg ggcgtaacca
    12901 gccagagaag gacaggtgcg acagacaaca aacgacgcat caggatctcc cactacgctt
    12961 aagcaaattt ttcatgaccg actgaaaatc ggtctgcggt atctcttctg ttggtgctga
    13021 agggggaagt ttatgcagca gattgatttg ccctgcggta acgccgagca ccagccgtgc
    13081 atcttccaca tcgaccacca caacccgttc acgcgcgccc agcgaagcac tggcgctaat
    13141 tttcagaccg ttaacgccag tgcgtttcgg ggcaaatccc aaccgtttta ccagccaggc
    13201 agcagcgagg atcagggcaa taatggcgat gagtgcgccg ctgacctgca gcagtggcgc
    13261 agcagaaacc ggcgtggaag attgcacagt agcgtggtta ttcatcacta acggctcagg
    13321 cgacgcattc gctcagacgg agtaatgata tcggtgatcc gcacgccata tttatcggca
    13381 acgaccacca cttcgccctg ggcgattaaa taaccgttga tcagaatatc cagtggttcg
    13441 cccgccagac cgtccagcgc cacgacggac ccttgcgtca gacgcaacag ctctttgatg
    13501 gtcatccgcg tacggcccag ctcgacggtc agcttgaccg gaatatccat tatcaggtcg
    13561 atatcctgca acgttccgct gacatcacca ccgccaaatt gctggaacac cgcgtcggca
    13621 gcgcttttgc tgctggttga tttttgttcg ctcaacgctt cagcccacag atcgtccatt
    13681 gcgccgttgt tgtcatcggc cggattattc atgtcactca tttgggctgt tcctcgttca
    13741 gagaatttaa aatcgggtta atcaaatgtt ctatccgtaa cgcatactga ccgttgagag
    13801 tgccgtactg actggtcagc accgggacgc cgtcaacatg ggcgatgatg ccatcgggtt
    13861 tttctatcgg caggacgtcg ccgggtttca gttttaaaat ctgcgacagg cgtagcgaga
    13921 tatcggcaaa gttggcgacc agctccagct gtgaatgctg cacctggcgc accaggttat
    13981 cgcgccagtt ctgatcttca ttacgcgagt tttccagcgg cgggttaacc aacaattccc
    14041 gtagcggctc gatcatgctg aatggcagac agatattaaa ttcgccggtc aggttgccaa
    14101 tctccacatg gaacggcgtg ttaaccacaa tgtcgttcgg cgaggtggtg atattggtaa
    14161 atttcacctg catttccgaa cgcacgtact caacttccag cggattaatc gccttccagg
    14221 cgtcgctata gccttcaagc gccagcttta acatgcggtt gatgacgcgc tgttcggtat
    14281 gggtaaactc gcgaccttcc actttggtcg ggaagcgtcc attgccgcca aacaggttat
    14341 ccacggcgat aaacaccaga ctcggtgaaa acaccaccag ccctgtgccg cgcagcggtt
    14401 tcagatggat aaggttcagg ttggtcggca ccggcaggtt gcgggcaaat tcatggtacg
    14461 gctgaatgcg gatggccccg acggttatat ccgggctacg acgcagcagg ttgaacagcc
    14521 ccatacgaaa atggcgggca aagcgctcat taatgatttc cagcgcctgc aaacgttcgc
    14581 gcacaacccg tcgttgggta ttcggatcgt acggacgaat gtcactttcg ccgctaacac
    14641 tggctgtcgg ttcgtctttg acttcgctgt caccattcaa cagcgcatca atttcagctt
    14701 gagaaagaat actatcgccc atgtcgttat cgcagaataa aagcggtata catcacgtcg
    14761 gtgacatcct gtttcggttg cccggcaaca agcggggtgg aaagcgtggt tttaatctcg
    14821 gcaatcaggt ttttcttgcc ttcttctgtc gccagtacgg cagcatcctg acgcgaaaac
    14881 agtaacagca aacggctacg gacttccggc aaatactcac tcagccgcga gcgggtagct
    14941 tcatctttga gtcgcagggt tataccgata taaagcacgc gatccgcatc gcccaaattg
    15001 accgtaaagg tatccagcgc atagaagacc ggtgagggca cgacgcgatg ctgcgctttg
    15061 tcttcagcgg caacctgatg cgaatgccag tagctgtaac ctgcgctggc acaggccgcg
    15121 agggtaatga ataccagaat cgggagccaa agcgatcgct tgcttttctt gcttatcgcg
    15181 taatcagtca tgtgttgcgg tcttcctgtg tcgctactgc ttatcggcta attatcctgc
    15241 gtcttgagcg caacaaagcg gggaaaagac gcggattacg gtgctacctc tgacgttagg
    15301 cgaaaatatc aacgccgctg ttgcctgtta cgcgcccttg taaagagacg ggaaccggaa
    15361 gcgtatcgtc ttcttccccc gccagaggtt catggtttac tgtgcgttgg ctttgctgtt
    15421 gttgggaagc ggcctgctgc tgaccactaa agctttcgcc actgatgttg ctttgcccta
    15481 actgaatgcc actctcggcc agctgcgtac gcagtaccgg cagcgctgct tccagggcgg
    15541 cgcgtacgtg ctgatgcggc gaaaccatct ggatttgcgc ctggttatca tccactttga
    15601 gggagatttg cacttcacct aaatcctgcg ggtgcagacg caactctgca ctttgttgtc
    15661 cctggcgggt gaacagcgaa atatgctggc ttaatgattg ttgccattcg tgagaaccca
    15721 gcggtgcact caagacaggc gcggcgacgg tgagcagtgg ctgtgtctgg tgtggagtga
    15781 ttagcgggct ggcggcagcg gtcaccggtg aaggtgtgct gatgacttcc gctttactct
    15841 gggcttctgc taccagcggt gttaatggct gagctggcgt gccgggggca tcatcaggct
    15901 gtgctgttgt gagttgcgca gaagtcagtt ttgtgaagag cgttggtttc tctgccggta
    15961 acacggttga cggcgcatca gtcactttgg gcgtattgtc aaaacccggc aacatcgcaa
    16021 aaagggcgct caggcttgcg gtgaggtctt cattcagatc atccgctttt tcgtcttttg
    16081 tcgtattttt gtcagccacc gcagccaacg tcatcgtatg agcggtggtt aacggtgtcg
    16141 atgtggattg ttcgacgttg atgacaggca gtgtttcatc cacagggatc agtaaatccg
    16201 cttgttgcgc gtcggaaaca atatcgctga tcagcagctc gccttttgtc gtgggcttat
    16261 ctgttgccac cagcacctgg ggggccgctt tgtcggtagt tgtctcgctt gctaatgctt
    16321 cgctcaacaa cgcgagaaaa tcttgtgcag catcgctggc tttgccgcca ggcaatgtgg
    16381 tggtgtcaac gtcggcggta atcaagggcg ctaagcgaat cattcaggtt tcctcatggc
    16441 ggcgcgctgg gcgaactcat ccatcttttt ctgatcgagg cggttttctg caagcagtgc
    16501 cgccgtggat tgccgttcct gcagtgtctg ccaggcctgc aaacgttgtt tcttttctcg
    16561 ccagctgttc agggcaatgt caactttctg cgtccactga ttaagttgct ggcgatgctg
    16621 agtaatggct ttttccagcg tctggataaa ctgctgatag ttgatccagc ggttgctggt
    16681 catcccggca ctcatatcgc tgttgaggtt attgcgatat tcattctgat aatcgatcag
    16741 cattttgagc tgttcttccg cctgctgaca cccgcgacgc atttcaccca gcaggcgcgc
    16801 ggcatcctct acctcttttt ctgccagatc tttcagggtc gccagcgcac catgttctgc
    16861 catctaccgt tatctcctgg gttatgacac tgtcgggaaa atacgctcca gcccctggag
    16921 agacgcttcc cagtccgcac gttcaaaaat gccttgttgt aaatagccct ccagctgcgg
    16981 ccacagggcg atggctttat cgagcatcgg atcgctgcct ttggcatacg cgccgacgct
    17041 aaccagatcg cggttacgct gaaaactcga caacagctgt ttgaaggtgc gcactcgcgc
    17101 gtaatgttgc tcactgatca acgccgtcat tgcgcggctg atcgacgctt caatatcgat
    17161 agccggatag tgcccggctt ccgccagtcg gcgagacagc acaatgtgac cgtcgaggat
    17221 cgcccgtgcg gagtctgcaa tggggtcctg ctggtcatcg ccttcagtaa gcacggtata
    17281 aaacgcggta atcgagccgc cgccgctaat gccatttccg gcacgctcga ccagtgccgg
    17341 taatttggca aacaccgacg gtggataacc tttggtggcg ggtggttcgc caatcgccag
    17401 cgcaatttca cgctgggcca tcgcgtagcg ggtgagggag tccataatca gcaacacatg
    17461 ctgaccacga tcgcgaaaat cttcggcaat gcgcgtggca taggcggcac cttgcattcg
    17521 cagaagcgga gagacatccg ccggagcggc aatcaccacg gagcgtgcac gcccttcagc
    17581 accgaggata ttctcaataa aatcttttac ttcgcgccca cgttcaccaa tcaaacccac
    17641 gacaatgaca tcggcgcggg tgtaacgtgc catcatcccc agcagcacac ttttaccaac
    17701 gccggacccg gcaaacagcc ccatacgctg cccacgccca acggtaagca gggcattgat
    17761 tgggcgcacg ccggtgtcca gcacatgttc aatcggtgta cgttgcaacg ggttaaatgg
    17821 cggggtaatc agcgcaccgg tttccgtcgt atcgggggag ggcaggccat cgagcggctt
    17881 accgctgccg tccagaacgc gacctaataa cgccggaccg agcggcaact gcttgccgct
    17941 ttgcagtcct tcagccgaaa tgtttttggc ataaacccgc gcgccgggca ggacaccttc
    18001 cacttcctcc agcggcatta aaaacagccg ttgaccgtta aagccaacga cttcgctttc
    18061 tacttcgtgc gtttcgctgc cgttctggcg ttcaatgaca caggttgcgc cgagcggcaa
    18121 ttgtaatccg gtggcttcca gcaccagccc ggtagcgcgg gttaatcgcc catagcgacg
    18181 taccgcaggc aactgcgcca ttttggcttc aaagttatcc agcgtggtta gccagcgagt
    18241 caggcgcgtg gtcattacac cactcctggt gctgccagac ggcagagttc ttgccagcga
    18301 gtggcgacac tggcgtcgag atcgccttca tcggcggaga ctttacagcc gccaggatgg
    18361 agtgtgggat cgccccgcaa gcgccagcca tgcaaactta aggttgcgcc gagcatatca
    18421 tccacacgtt gcagatcatc cgggtgcacg cgcagctgtg gtttaccgct gaataacggt
    18481 tcttgctgca acaactgttg gatctgtttg atcagtgccg agttatccac tgttggcgtc
    18541 tgaccgatca cctgacgtgc cgcctccagc gccatctgca tcaggcgcga agcaatcaca
    18601 ctatcaagcg catcaagggt agtttgaaat tcgctgacca gttgctgcat ccgggcatga
    18661 attggcgctt gttgagactt cgcctctgcc agaccttgct ccagcccctg ggccagtcct
    18721 tcctgatagc cctgctcatg accttgctgg cgaccttcgg caatccccgc ctgataacct
    18781 tgctcatggg cctgcatttg cagttgcgcc agttgctgct caaggctagg ttcggcctct
    18841 tcaatgatgg tttcttccgg ctcgaccatg ggcacaaact ctgcctgtgg tggcgcgaga
    18901 tcgtccggcg tccaggtttt ccacggcaga ttatcagaca taggtatcct cgccgctgcc
    18961 aattaccatc tcgccagttt cggcaaggcg gcgcacaatc agcagaatcg ctttctgttc
    19021 gttttccacc tgcgacagac gcaccggacc acggttggcg agatcgtcgc gcagaatatc
    19081 ggcggcacgc tgcgacatat tgcgcaggaa tttctcgcgc agtggctgct cggccccttt
    19141 cagcgcgatc aacagcgatt cggaatccac ttcctgcaac agacgctgaa tgctgcgatc
    19201 gtcgacatcc accagatttt cgaacaggaa catctcgtcg ataatttttt gcgccagctc
    19261 gccgtcgaat tcgcgcacgg cggtaataac ggcttcttcc tgctgagttt tcatcaggtt
    19321 gataatttcg gctgccgttc tcacgccgcc cattttgctg cgcttgagat tctgaccgtc
    19381 gagcaagcca ttcagtactt cggtcagttc cgccagcgcg gctggctgca cgccgccaaa
    19441 ggtggcgata cgcaacatca cgtcgtggcg cagacgttca tcgaacaacg ccagaatatc
    19501 ggcggcctgc gcgcgcttca gatgcaccag aatggtggcg ataatttgcg gatgctcatc
    19561 gcgaatcaga tcggcggcac tctgcggctc cataaagttg agcgtttcaa taccgctggc
    19621 ggtatcgcga gtttcgagaa tatcttccag caggctggcg gcacgttcct cacccagagc
    19681 tttgaccaat accgaacgca gataatcgtt ggcgttgata ttcagtgcgg caaactgttc
    19741 agcttcttgc tcaaactccg ccagcacata ggttagctgc ttgttggaga tctgcgtgac
    19801 gttcgccatt gcagcgctca gggtttgcac ttcgcgctgg gagaggtgct tgaacacctc
    19861 tgccgcccgg tcttcgccaa tggtcatcag caggatgacg cttttatcgg tgcctgtcag
    19921 gttactcatg atcgttattt atccactggc gaatgaccag cgccaccacg cgcggatcgt
    19981 tatcagacat ttcacggata cgctggctca tgacttctgc ccccagacgt tggttagcgc
    20041 gtcgttgttg gagttgttcg tctttgctca ggcggacttc caccgcatct tccacttcct
    20101 cacgggcctg cgcctgttgc tgtacagctt tcatcgcctc ggcgcgacgt gttaactgcg
    20161 ggcgtaccgc tttccgccac agcagccacg ccaccagcag taccagcaac cagcgaccgg
    20221 cagccagcaa ctgatcgata aacgcttgct gttgccagaa cggcagttct ccgccgcttt
    20281 cgtcactgct attgaacggc gagttaacga cattgagcga gtcaccgcgt ttttcagaaa
    20341 agcccatcgc ctcgcgggtc agatcttcaa tttgcttcat ctgttcgttg ctgagaggca
    20401 atggtttacc gtctggcaag gttttgtaat tcaccactac cgcgactgac agacgttgca
    20461 catcgcccac gttcattttg gtatgacgaa tggtgcgatc gacttcgtag ttactggttt
    20521 cattccgctg tgtgctacgc ggcccgctat tgctggtggt gctcgcctgc tgctggcggt
    20581 tattttgatt tgccggaggc gtgctgattg gcgcgttatt cgcaggtgcc ggttgattcg
    20641 acaacgcccc cggtacgccg cccggataac cggaaccgct ttgctcgctc tcattaagct
    20701 ggcgtgaacg aagcgccgca tgagattcat caccgttagg gcgatactgt tcttccgttt
    20761 gttctttact ggcgaagtcc agttgcgccg tgacctgggc gtgaatatta ccgttaccaa
    20821 caataggcga caggattgct tcaatacgcc gctgaatacg gccttcgaca tcgctggcat
    20881 atttcaactg agcgtcatta agatcgcgcc cgctggtatt ggactgggtt aacagatgtc
    20941 cgccctgatc caccagcgtg acgtttcccg gcggcagacc agcaacggcg ctggaaacca
    21001 ggtgcacaat ggcgctaatt tgcccttcat cgagtgcgcg gccgggtaac agatttaccg
    21061 tcaccgatgc agaaggggat ttttgttcac ggacgaataa agacggtttc ggcattgcca
    21121 gatgtacgcg cgcccctttt accgggccga tagtttcgat ggtacgagaa agctcgcctt
    21181 ccagcgcccg ctgataattc acctgttcgc tgaactggct gataccaaac ttttcctgat
    21241 caagcagttc gaaaccgacc gcgccgcctt tgggcaaacc ttgttgtgcc aggcgcagac
    21301 gcagttcgtg aactttatct gccggaactt caatagcgcc gctggcttcg ctgaagcagt
    21361 aaggaatatt catttgcgtc agttggctga caattgcgcc accatcctga tcggaaagat
    21421 tgctgaataa tgtgcggtag tcgggggctt tcgcccacag gatcagtgcg accatgaccg
    21481 ccactgccgc ggaaccggca acaatcaatg gaattttcgg attcgcacgc aggcgattaa
    21541 gccactcaag agattttgtc tgggctgcag tcgcattcat cgcgcacctc gtggctgatt
    21601 atggacggcg ttattggcaa agtggaacaa gactcactcc cgggtcagca aactcgaaaa
    21661 attgacgctg ccattatttg tgctttcgtt tttttctatc cgccaataaa cccgtttttt
    21721 ttgttgctat ttagcgcctt tgtcttattg acttactggt aagctttgct accagaaatt
    21781 atccgggaga tgaaaatgtc agcgatacag gggattgaag gggttatcag ccagttacag
    21841 gctacggcga tgagtgcgcg tgcgcaggaa tcactgccac aaccgaccat tagttttgcc
    21901 gggcagctgc acgccgcgct cgatcgcata agcgatacac aaacagccgc ccgcacgcag
    21961 gcagaaaaat tcactctcgg tgaacccggc gtggcgttaa acgatgtgat gaccgatatg
    22021 caaaaagcct cagtttctat gcaaatgggg attcaggtgc gtaataagct ggtggcggct
    22081 tatcaggaag tgatgtccat gcaggtgtag gttttgtaac ctgttgttaa ttacatccga
    22141 tctcaccgcg tggggcatgg atggggcaaa ctcactcaat ttctggttta aaatggatac
    22201 ctgatcctga aagttttctg ccatccacga tccgtaaacg cgataacatg ttttgtcttc
    22261 gctttgctca taggaagagt gaactctttt gttagcctgg ggttccttct gatcattatt
    22321 gttcctgcct ttatctcttg ccacgccaga gccccctgga tacacataca ccaggatgaa
    22381 aacggtgagt tgtgttcaaa ctgtagtaca attctctcca gtatgaacag gaaagaatat
    22441 gctatgaacc cttatattta tcttggtggc gcaatacttg cagaggtcat tggtacaacc
    22501 ttaatgaagt tttcagaggg gtttacacgg ttatggccct ctgttggcac aattatttgc
    22561 tattgtgcat cattttggtt attagctcag acactggctt atatacctac agggattgct
    22621 tatgctatct ggtcaggagt cggtatcgtt ctgattagtt tgctttcctg gggatttttt
    22681 ggccaacgac tggacctgcc agccatcata ggcatgatgt tgatttgtgc cggtgtgtta
    22741 gtcattaact tgttgtcacg aagcgcaccg cattaaaaaa taatcatcct tgagataaca
    22801 ggaatctgat ttgtgcatat tatggctaat gttcggggca actttataat tatgattgca
    22861 tttgcgcgat cattgcgcct ttttgcataa tgagatcaat gcacatcaaa taaaataacg
    22921 ataggtcaca acaaagaggt ttttatgaaa aaacttatcg tttcaagtgt attggcattc
    22981 ataacatttt ctgcgcaggc cgcagcattt caggtcacta gtaatgaaat aaaaacagga
    23041 gagcaactta caacgtctca tgtattttct ggagttgggt gtgagggggg taatacatcg
    23101 ccctcattaa cctggtctgg tgctccagaa ggcaccaaaa gctttgccgt aactgtgtat
    23161 gatccagatg cgcctacagg cagcggatgg tggcactgga ctgttgctaa tattccagca
    23221 acggtaacat atttgcccgc tgatgcagga agacgtgatg gtacaaaact tccgactggt
    23281 gctgtccaag gccgaaatga ttttggctat gcgggatttg gtggagcatg tcctcccaaa
    23341 ggagataaac cccatcatta ccagtttaaa atatgggctc taaaaactga taagattcct
    23401 gtggattcta attccagcgg agcgttagtt ggttatatgc ttaatgccaa taaaattgca
    23461 accgctgaga taatacccgt ttatgaggta aagtaggata agagtatgct ggcaaaagat
    23521 aggactaacc taaagattga agaaatacgg atgcataaac atcatgagat tcacagggtt
    23581 aagcctctta tgccagcttc atgtcgtatc cgtcagggaa aaaaagttat caattgggag
    23641 acgcatagtc taactgttga taataatcag ataatattat ttccctgtgg atatgaatct
    23701 tatattgcga attacccaga agcaggtc
//
LOCUS       DAESHI010000060        23539 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.060, whole genome shotgun sequence.
ACCESSION   DAESHI010000060 DAESHI010000000
VERSION     DAESHI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23539
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.060"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..2064
                     /locus_tag="KW011_002454"
     CDS             <1..2064
                     /locus_tag="KW011_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBH7660763.1"
                     /translation="GYVPPENWERGINAFYTSYYVSQYYSDYKASGNSKSTYVRFNSG
                     LNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSVR
                     FRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITDL
                     QLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHSD
                     FVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGDV
                     FDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYDI
                     ADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLRR
                     ISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFASN
                     NTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQAG
                     ASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMTP
                     YRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQPL
                     TFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDESR
                     NYICQ"
     gene            2080..3099
                     /locus_tag="KW011_002455"
     CDS             2080..3099
                     /locus_tag="KW011_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HBH7660764.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNANLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGNKATIN
                     SKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFMLESNGS
                     PVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALEATALI
                     NVKYD"
     gene            complement(3181..3519)
                     /gene="rcnB"
                     /locus_tag="KW011_002456"
     CDS             complement(3181..3519)
                     /gene="rcnB"
                     /locus_tag="KW011_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HBH7660765.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3738..4562)
                     /gene="rcnA"
                     /locus_tag="KW011_002457"
     CDS             complement(3738..4562)
                     /gene="rcnA"
                     /locus_tag="KW011_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HBH7660766.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            4683..4955
                     /gene="rcnR"
                     /locus_tag="KW011_002458"
     CDS             4683..4955
                     /gene="rcnR"
                     /locus_tag="KW011_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HBH7660767.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      5047..5143
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5178..5966
                     /gene="thiM"
                     /locus_tag="KW011_002459"
     CDS             5178..5966
                     /gene="thiM"
                     /locus_tag="KW011_002459"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBH7660768.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            5963..6763
                     /gene="thiD"
                     /locus_tag="KW011_002460"
     CDS             5963..6763
                     /gene="thiD"
                     /locus_tag="KW011_002460"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBH7660769.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6828..7646
                     /locus_tag="KW011_002461"
     CDS             6828..7646
                     /locus_tag="KW011_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HBH7660770.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            7698..8444
                     /locus_tag="KW011_002462"
     CDS             7698..8444
                     /locus_tag="KW011_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH7660771.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8418..9383)
                     /locus_tag="KW011_002463"
     CDS             complement(8418..9383)
                     /locus_tag="KW011_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBH7660772.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9380..10384)
                     /locus_tag="KW011_002464"
     CDS             complement(9380..10384)
                     /locus_tag="KW011_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HBH7660773.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(10381..11658)
                     /locus_tag="KW011_002465"
     CDS             complement(10381..11658)
                     /locus_tag="KW011_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH7660774.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11915..12967
                     /gene="fbaB"
                     /locus_tag="KW011_002466"
     CDS             11915..12967
                     /gene="fbaB"
                     /locus_tag="KW011_002466"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HBH7660775.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13286..14140
                     /gene="gatY"
                     /locus_tag="KW011_002467"
     CDS             13286..14140
                     /gene="gatY"
                     /locus_tag="KW011_002467"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBH7660776.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14169..15426
                     /gene="gatZ"
                     /locus_tag="KW011_002468"
                     /pseudo
     CDS             14169..15426
                     /gene="gatZ"
                     /locus_tag="KW011_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            15436..15888
                     /gene="gatA"
                     /locus_tag="KW011_002469"
     CDS             15436..15888
                     /gene="gatA"
                     /locus_tag="KW011_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HBH7660777.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            15919..16203
                     /gene="gatB"
                     /locus_tag="KW011_002470"
     CDS             15919..16203
                     /gene="gatB"
                     /locus_tag="KW011_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HBH7660778.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            16207..17562
                     /locus_tag="KW011_002471"
     CDS             16207..17562
                     /locus_tag="KW011_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBH7660779.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            17610..18650
                     /gene="gatD"
                     /locus_tag="KW011_002472"
     CDS             17610..18650
                     /gene="gatD"
                     /locus_tag="KW011_002472"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBH7660780.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            18756..19529
                     /locus_tag="KW011_002473"
     CDS             18756..19529
                     /locus_tag="KW011_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBH7660781.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(19611..20510)
                     /gene="yegS"
                     /locus_tag="KW011_002474"
     CDS             complement(19611..20510)
                     /gene="yegS"
                     /locus_tag="KW011_002474"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HBH7660782.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
     gene            20916..21233
                     /locus_tag="KW011_002475"
     CDS             20916..21233
                     /locus_tag="KW011_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660783.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(21563..22924)
                     /gene="yegQ"
                     /locus_tag="KW011_002476"
     CDS             complement(21563..22924)
                     /gene="yegQ"
                     /locus_tag="KW011_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HBH7660784.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(23071..23403)
                     /locus_tag="KW011_002477"
     CDS             complement(23071..23403)
                     /locus_tag="KW011_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HBH7660785.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
BASE COUNT         6069 a         5513 c         6029 g         5928 t
ORIGIN      
        1 ggctatgttc caccggaaaa ctgggagcgg ggtattaatg cgttttatac ctcttattat
       61 gtgagtcagt attacagtga ctataaagcg tcgggtaata gtaagagtac atatgtacgt
      121 tttaacagcg ggttaaattt actggggtgg caactgcatt ctgatgccag tttcagtaaa
      181 acaaataaca atccaggggt gtggaaaagc aataccctgt atctggaacg tggatttgcc
      241 caacttctcg gcacgcttcg cgtgggtgat atgtacacat caagcgatat ttttgattct
      301 gttcgcttca gaggtgtgcg gttgtttcgt gatatgcaga tgttgcctaa ctcgaaacaa
      361 aattttacgc cacgggtgca ggggattgct cagagtaacg cgctggtaac tattgaacag
      421 aatggttttg tggtttatca gaaagaggtt cctcctggcc cgtttgcgat tacagatttg
      481 cagttggccg gtggtggagc agatcttgat gtcagcgtga aagaggcgga cggttcggta
      541 accacctatc tggtgcctta tgcagcggtg ccaaatatgc tgcaacccgg cgtgtcgaaa
      601 tatgattttg cggcgggtcg tagccatatt gaaggggcga gcaaacatag tgattttgtc
      661 caggcgggtt atcagtatgg ttttaataac ttattgacgt tgtatggtgg ctcgatggtt
      721 gcgaataatt attacgcgtt cactttgggg actggctgga atacacgcat tggtgccatt
      781 tccgtcgatg ccacgaagtc gcatagtaaa caagacaacg gcgatgtgtt tgacgggcaa
      841 agttatcaaa ttgcctacaa caaatttgtg agccaaacgt cgacgcgttt tggtctggcg
      901 gcctggcgtt attcgtcgcg tgattaccgg acatttaacg atcacgtttg ggcaaacaat
      961 aaagataatt atcgccgtga tgaaaacgat gtctatgaca ttgccgatta ttaccagaat
     1021 gattttggtc gcaaaaatag cttttccgcc aatatgagcc agtcattgcc agaaggttgg
     1081 gggtctgtgt cattaagtac gttatggcga gattactggg ggcgtagcgg cagtagtaag
     1141 gattatcagt tgagttattc caacaacctg cgacggataa gctataccct cgcggcaagc
     1201 caggcttatg acgagaatca tcatgaagag aaacgtttta atatttttat atcgattccc
     1261 tttgattggg gtgatgacgt ttcgacgcct cgtcggcaaa tatatatgtc taactcaacg
     1321 acgtttgatg atcaggggtt tgcctcaaat aatacgggat tatcaggaac agtagggagt
     1381 cgggatcagt tcaattatgg tgtcaacctg agtcatcaac atcagggaaa tgaaacgaca
     1441 gctggagcga atttaacctg gaacgcgccg gtggcaacag tgaatggcag ttatagtcag
     1501 tcgagtactt atcgacaggc tggagccagt gtttcagggg gcattgtcgc ctggtcgggt
     1561 ggcgttaatc tggcgaaccg tctttccgaa acgtttgctg tgatgaatgc gccaggaatt
     1621 aaagatgctt atgtcaatgg gcaaaaatat cgcacaacaa accgtaatgg agtggtggta
     1681 tacgacggaa tgacacctta tcgggaaaat cacctgatgc tggatgtgtc gcaaagcgat
     1741 agcgaagcag aattacgtgg caaccggaaa attgccgcac cttatcgcgg cgcggttgta
     1801 ctggttaatt ttgataccga tcagcgcaag ccctggttta taaaagcgtt aagagcggat
     1861 gggcaaccat taacgtttgg ttatgaagtc aatgatatcc acggtcataa tattggcgtt
     1921 gtcggccagg gaagccagtt atttattcgc accaatgaaa taccgccatc ggttaatgta
     1981 gcaattgata agcaacaagg actttcatgc acaatcacct tcggtaaaga gattgatgaa
     2041 agtagaaatt atatttgcca gtaatagtca ggtggttcta tggaaattcg cataatgcta
     2101 tttatattaa tgatgatggt tatgcctgtg agctatgcgg catgttatag tgagttatct
     2161 gttcagcaca atttggttgt tcagggggat tttgcactta ctcaaacaca aatagcgaca
     2221 tatgagcata attttaatga ttcgtcatgc gtgagtacaa atactatcac ccctatgagt
     2281 tcgtcggata ttattgttgg gctttataac gataccataa aattaaattt acattttgaa
     2341 tggaccaata aaaacaacat cacgttgtca aataatcaga ccagtttcac cagtggttat
     2401 tcagttacgg tgacacctgc ggccagtaat gcaaaagtga atgtttctgc ggggggcggc
     2461 ggttcagtga tgattaatgg tgttgcgaca ttatccagtg cttcatcatc gacacgcggg
     2521 agtgccgcag tacaatttct actgtgttta ttaggtggca agtcatggga tgcatgtgta
     2581 aatagctaca gaaatgcatt ggcacaaaat gctaatctga cattgtcata caacccgata
     2641 accacaacct gcaaaccgga cgatttatta attactttag acagtattcc cgtttcacaa
     2701 ttaccagcca caggtaacaa agcaacaata aatagtaaac aaggggatat tattctgcgt
     2761 tgtaaaaatt tattaggtca acaaaatcaa acatcacgga aaatgcaggt gtatttatca
     2821 agttctgatt tgttagccgg cagtaacacg atcctgaaag gtgcagaaaa taatggagtc
     2881 ggatttatgc ttgaaagcaa tggttctcca gtcacatttt taaatatcac tgataacagc
     2941 aaaggataca caaatttaaa agaaattgcg gcaaagtcaa aacttacaga tacaacggtt
     3001 tcaattccga taactgccag ttactacgtc tacgatacaa acaaagttaa atctggcgca
     3061 ctggaggcaa ccgcattaat caacgtgaaa tacgactaat aacaagcaag acgagcaagt
     3121 ggctaataat aaaaaataac aaggtgtctg catttccccc tcatgatgag ggggcttttt
     3181 ttagcgatga taaaaaatct caccgtcgta ggctttaatg attttaccgt cggtgtcgct
     3241 gatcaacacg tacgcgccac ccatataggt ccagtgcgtc ccggcatcag gcgcgggcag
     3301 gttacgtaac tgccactgct taatgttgta ctcatcggtg cggtacatct ctgggacggt
     3361 atcacctggt ttgaaatgct taaagtcagc aatgaaacta cttaactcat acttagaaat
     3421 tcccgaggta ttggtcgaac ccgcggtggc tggtgcggcc caggcggcac tggtaaccag
     3481 caaaagcaca cccagcaaca tcttattttt aatggtcata aactctccac tttggggatc
     3541 tgtaatatca gtcgcatatc tttcctgcca tacaatataa ctgacaagcg atattttgtt
     3601 agtgatccca tgatttcgta aaaagtgtaa atgaaaggat gccctaaaag catccttagc
     3661 gagcagagag tcagcggaaa tagctgattt aagaatcatc ctgggctgaa gataacatgc
     3721 aagcctaaag gattgagtta tcgcattatg cccatgaagc catgtacgcc catatacaca
     3781 ccgactaagc caatcaacag actggaaaaa tagggggcgc gtttagcgag agtgttaaat
     3841 ccgctccagc gttttgcgac ctgctgaacg ctgattgctg cgccaacgcc tacggtgaca
     3901 agcgttaacg ccaggccaat gctgaaactg acgaccagtg ttgcgcccag tgtcagggct
     3961 ttcaactgaa tgcatatcaa cagcacggta attgctgccg ggcaggggat aaggccaccg
     4021 gttaagccaa atagcaaaat ttgccagttg gtgacctctc taccatcaaa gcgtcgttta
     4081 atgtcattgg catgggctcg tgcatgggca tcctgatact cgccatgttc gtgatggtga
     4141 tgatgtccat ggtcgtggtg atcttcgtga tcgtgatgat gatgctcata atcatactcg
     4201 tgcatattct ccagccagtt gcgttcgccg cgccaggtac gccagaacat ccagaacgcg
     4261 gtgccaataa tgatcactgc ggaaatcagc tggagccacg gttctgctga ttgagcagta
     4321 aagcgcttgc tgatcaccat cccgccaaag gcaattaacc agaccactgc ggtatgcgaa
     4381 atagttgctg ccagtccgag catcaccgct tgtttaatgg tgcctttgat ggcgatgata
     4441 aacgccgcca tcatcgtttt tgagtgccct ggttccaggc catgaagcgc accaagtaag
     4501 atggcgctgg ggatgaagaa ccaggcgttt ccttgctgaa gaagagttgt aaattcggtc
     4561 atgataataa ttcttagtat taattcggca atctgattct actcccccca gtacctgata
     4621 ctacccccca gtagattaat agtgctatga tattttcata ttcttgttaa ccaggtgttg
     4681 ccatgtctca tacaatccgt gataaacaga aactgaaagc gcgtgccagt aagattcagg
     4741 gccaggtcgt ggcgctcaag aaaatgctcg acgagccgca tgaatgcgct gcagttttac
     4801 aacagattgc tgctatccgt ggcgcggtaa acggtctgat gcgggaagtg attaaaggtc
     4861 atctgacgga acacatcgtt caccaggggg atgagctaaa acgtgaagaa gatctggatg
     4921 tcgttctgaa ggtgctggat tcatatatca aataatttat taacgcgatt gtaaaactgc
     4981 cgtttttcct cgtttacaac gcgtgcgctg gacattacca tcctcctctg cgatttatca
     5041 tcgcaaccaa acgactcggg gtgcccttct gcgtgaaggc tgagaaatac ccgtatcacc
     5101 tgatctggat aatgccagcg tagggaagtc acggaccacc aggtcattgc ttcttcacgt
     5161 tatggcagga gcaaactatg caagtcgacc tgctgggttc agcgcaatct gcgcacgcgt
     5221 tacacctttt tcaccaacat tcccctcttg tgcactgcat gaccaatgat gtggtgcaaa
     5281 cctttaccgc taataccttg ctggcgctcg gtgcatcgcc agcgatggtt atcgaaaccg
     5341 aagaggccag tcagtttgcg gctatcgcca gtgccttgtt gattaacgtt ggcacactga
     5401 cgcagccacg cgctcaggcg atgcgtgctg ccgttgagca agcaaaaagc tctcaaacac
     5461 cctggacgct tgatccagta gcggttggtg cgctcgatta tcgccgccat ttttgtcatg
     5521 aacttttatc ttttaaaccg gcagcgatac gtggtaatgc ttcggaaatc atggcattag
     5581 ctggcattgc taatggtgga cggggagtgg ataccactga cgccgcagct aacgcgatac
     5641 ccgctgcaca aacactggca cgggaaactg gcgcaatcgt cgtggtcact ggcgaggtgg
     5701 attatgttac cgatggccat cgcgccgttg gcattcacgg tggcgatccg ttaatgacca
     5761 aagtggtagg aactggctgt gcattatcgg cggttgtcgc tgcctgctgt gcgttaccag
     5821 gcgatacgct ggaaaatgtc gcatctgcct gtcactggat gaaacaagcc ggagagcgcg
     5881 cagtcgccag aagcgagggg ctaggcagtt ttgttccaca tttccttgat gcgctctggc
     5941 aattgacgca ggaggtgcag gcatgaaacg aattaacgct ctgacgattg ccggtactga
     6001 tccgagtggt ggtgcgggga ttcaggccga tcttaaaacc ttctcggcac ttggcgctta
     6061 tggttgctca gttattactg cactggtggc gcaaaatacc cgtggcgtac agtcggtgta
     6121 tcgcattgag cctgattttg tcgccgccca gctcgattcg gtgttcagcg atgtgcgaat
     6181 cgataccact aaaatcggta tgttggcgga aaccgatatt gttgaagcgg tggcagaacg
     6241 gttgcaacgt tatcagatcc aaaacgtggt actcgacacc gttatgctgg caaaaagcgg
     6301 cgacccactg ctttcacctt cggcagttgc cacgctacgc agtcgattat tgccacaggt
     6361 ttcattaata acgccaaact tgcccgaagc agccgccttg ctcgacgcgc cacacgcgcg
     6421 caccgaacag gaaatgctgg aacaagggcg atcgctgttg gcgatgggct gtggcgcagt
     6481 gctaatgaaa ggtggtcatc tggatgatga gcaaagcccg gactggctgt ttacccgcga
     6541 gggtgaacaa cggtttaccg caccgcgcat tatgaccaaa aacacccacg gtactggttg
     6601 tacactctct gcggcgttgg ctgcactacg cccgcgccat acaaactggg ctgacaccgt
     6661 acaggaggca aaaagctggc tttcatcggc gttagcccag gccgacacgc tggaagttgg
     6721 tcacggtatt ggtccggttc accacttcca cgcctggtgg tgagcaccgt actatagctg
     6781 ctttggttat tgtcagcctt tggcactgac attcagggat gaaaccaatg cagctaagaa
     6841 ttaccagtcg aaaaaaactc accgctctat tatgcgctct tgggctaatt tccatcgttg
     6901 caatttatcc gcgtcagacg gtgaattttt tctattcgac agcagttcag attacagact
     6961 acatccactt ctacggttat cgcccggtta aatctttcgc tattcgtatt cctgccagtt
     7021 acaccattca cggaatagat gtttcgcgct ggcaggagcg gatcgactgg cagcgtgtgg
     7081 caaaaatgcg cgataacggt attcgcttac aatttgcctt tattaaggcg acggaaggcg
     7141 aaaagctggt ggacccctat ttttcgcgta actggcaact aagccgcgaa aatggcttgc
     7201 tgcgcggggc atatcattat ttctccccgt cggtagcagc accggttcag gtgcgattat
     7261 ttctgcaaac ggtagatttc tcacaaggcg atttccctgc ggtactggac gtagaagagc
     7321 gaggaaaatt atcggcaaaa gaattacgca agagggtaag tcagtggcta aaaatggtcg
     7381 aaaaaagtac ggggaaaaag ccgattattt actcaggagc cgttttttat cacacgaatc
     7441 tggcgggcta tttcaatgaa tatccgtggt gggtggctca ctactatcaa cgtcgtccgg
     7501 acaatgacgg catggcctgg cgcttctggc agcattccga ccgtggacag gtagatggca
     7561 tcaatggtcc ggtggatttt aatgtgttta atggcacggt ggaggagttg cagggattcg
     7621 ttgatgggat taaagaaacg ccttaaataa gaagcagtga tatactccca ccagactccc
     7681 tggcaggaaa aagacaaatg gaacaagcgc atacccagct gattgcccaa ctgaacgaac
     7741 gtattttagc ggcggataac acgccgcttt atattaagtt tgccgaaacg gtaaaaaatg
     7801 ccgtgcgcag cggggtgctg gagcatggca atattttgcc cggtgagcgt gatttaagtc
     7861 agttaaccgg cgtttcgcgc attacggtgc gcaaggcgat gcaggcgctg gaagaagagg
     7921 gcgtggtgac gcgttcgcgc ggttacggca cgcagatcaa caacatcttc gaatactcgt
     7981 tgaaagaagc gcgaggtttt tctcagcagg tggtgttgcg cgggaaaaag cccgatacgc
     8041 tatgggttaa caagcgtgtc gtgaaatgtc ccgaagaagt cgcgcagcag ctggcggtcg
     8101 aagcaggaag tgatgtcttt ttgcttaagc gtattcgcta tgtcgatgaa gaagcggtat
     8161 cgattgagga atcgtgggtt ccggcgcatt taattcatga tgttgatgcc atcgggattt
     8221 cactttatga ctatttccgc agccagcaca tttacccaca acgtacgcgt tcccgcgtta
     8281 gcgcccggat gccggatgcc gagtttcagt cacatattca gttagatagc aaaataccgg
     8341 tgctggtgat caagcaagtg gcgcttgacc aacagcaacg gcctattgag tacagcatta
     8401 gccactgtcg cagcgatcta tacgtttttg tgtgcgagga gtagttcctc gcgcgttggc
     8461 gcacaatcac cgccccgatg cccgacaacc cacgacgcca ctgcattgcc cagtaatacg
     8521 gcatccgcca gtggcagacc agaggccagc ccggcaagta cgccacctgc atgactgtcg
     8581 cccgccccaa tggtgtctac aacttgcgtt ggaaatgccg gaatgcagcc agaagcgtcg
     8641 ttgctgaaat accatgcgcc ttctttatcg agacgaacga tcaacggcgc ggcaaatttc
     8701 tcctgccatt gctcgccaag tgttgttatc tctgcggata aagcaaaacg ttcggcggca
     8761 atctcagcct cttgacgatt gagcgacact aaaggtcgac aggccatgat ccgcgccagt
     8821 aatgcatccg ggatatcgcc aatacgtggg ccaaaatcga taaacggcgt cacgtcttgc
     8881 agctcttcca gccattccac taacaattcg ccgcagggcg aggccagttg ataaccggaa
     8941 aaatagagca ggctgccagg cgcaacggtt aatcgcgcca gccactggcg attccactga
     9001 ttttccacac cgctgaatga cataaaagtg cgttcgccat ccggctcaac cagcgccaga
     9061 caccagccgt tatcaccttc ggcgttatcg atcaggctga ttaagccctc ttttgccatc
     9121 cggttgcgaa taatctccgc ccacacgccc tgaccgagcg gcaaggcatt acccgcttcg
     9181 atgccgaggc gctttaacgc cacggcaata ttcagtgcgc agccgccaac gttaacgctc
     9241 tgctgtttca gttcgatatc gcacccacgc caggggaggg cataagcgtc ggcgatcacg
     9301 tcaatgaccg ccgcgccgac gaccatcacc ggctgacgcg tggttaattc aggcagcagc
     9361 gtgtgtaatc gagcgccgct cataccgcct cccgttgttg acgatatttc gccagcgctg
     9421 tggcatagcg gttgaaatca agctgattta ccgcatccag ttccgccttt aacgcaggat
     9481 cgatagcgtt aacgccatgc aacgcgccgc aaattgccgt cgccatagca ccgatggtgt
     9541 ctgtgtcgcc gccaaggtta gcgcacagga cggcgcagcg attcgggtcg gtttgtgcta
     9601 gttcaaccag cgcaatggcg cacggaacgg actcaatagt gctggtacct gcgccaacga
     9661 cctggtaaag ctgttcgctg gcggattcgg tgccgtcggc attgcgcaca attttcagcg
     9721 caatctccag ccgcgccgcc agtgaggcgc tgaacgtagt gatgcgtttt tgctgtgcat
     9781 gtcgcgcaat tgaaggcagg gaatcaacaa tcgctgacca gctttctccg tcaatggcac
     9841 gagaaatcgc ccatgcgatg actaccgcgc ctgcaaccgc cagatcggat ttatgggtcg
     9901 ggctggacgc cagcgctaca tcatcaataa aggaatcaac atcacgcgcc ggaagcaaac
     9961 aacctaatgg cgagacgcgc atcgctgcgc cgttggtcac gccgttgttt tccagttcag
    10021 caacgggttt accgtcgcga atggcgttaa gcgcaatctt cgaggtcgga cctagtacgt
    10081 ttttgttaaa ggcgtcgaaa cgcagcgccc agtcgagaat attacgccca atcagatccg
    10141 gatcgatctt gccttcacgt tccagtaacg catccgccag acacagcgcc atcgaggtat
    10201 cgtcggtgaa ttcggcgcgg ttaaaataac aggccgcgtt attctccttt ggtccaggaa
    10261 gaaaacggtc aatccagcca aagtgtgctt taacgcggct gcgtggccaa agctcggagg
    10321 gcatccccat cgcatcccct aacgcctgcc cataaagagc accgagaata cgttctgttt
    10381 tcatttaact tccccttgtg tcaacgcaat atcgcgatca tcgaccttga tagccgtaat
    10441 ttcgttgtcg gattcgcgga aaaaaatcat gaacagcacg gcgataatgg caatcatcac
    10501 cgcgccgaaa gtccacatcc cggaccagtt gaaagtcagt ccgtttaccg gttcctgata
    10561 agcgaacatc ttttccatca tcacaccgcc aagacgatag ccgagcaaac tgccgaagcc
    10621 ctggcagcag agcgtgatca gcccctgcgc agcggtacgc atatgcacgg gggctttttt
    10681 atcgacatag atgtaagcgg taacgtagta aaaatcgtaa cttacgccgt gaagcaaaat
    10741 accgaggaac agtaacgcgt aggtgaaata ttcatccgta ctaccgtaaa taaagaagcc
    10801 atagcggatc gcagcggtga ccagaccaag caataatacc tttttgatac caaagcgttt
    10861 agtgaaaaac ggcaatgcca gcataaagaa gatttcagag aactggccga gcgtcatcca
    10921 gccggtggcg tttttcatgc caacttcggt cagataaccg ttggcaaaga tgtaatagaa
    10981 cgccagtggc atcgcaaaca ggaatgaaca gaagaaaaag acgaggaagt ttttatcgcg
    11041 cagcaggatc agcgcatcca ggccgagcat gactttaata tccattttgc cggtgctttt
    11101 tggtggcgtg tcgggcagga aaaacgcaaa cacaccgagc agagcagaac ttccggcggt
    11161 aatcagcagc gggatgttag tcggtgagat atcggcatac cccagtattt gcggcaagaa
    11221 accacatgcc agaccggagg caatccagcc gatagtgccc atcacacgaa tgcgcgggaa
    11281 atcacgctcc acatccggca cgttggcaaa agcgatgctg ttagtcagcg caatggtcgg
    11341 catataggtt agagagtaag ccagcagtaa cggaaagaac ccggcaaaag tggtctgttg
    11401 cgcagcgaaa tacatcagca gcgcgcctgc gaacatcaat accgccagca ctttttgcgc
    11461 cgagaaaaag cggtcagtga tggagccaac cagaattggc gacaggatcg ccgcaatggc
    11521 ggtacaggca tacgaccagc caatttctcc ggcactaaaa ccgcttttac ttaaccataa
    11581 ccacaatggc acaaaccacg cgccccagat aaaccattca acaaacatca tgaacgacag
    11641 ctttgctgtt gttttcatct tttaattcct tcatgacagg taatgtaagg tacgcgatga
    11701 caataccact taacaatacc ttttaaatac ctttgcggtg aaaacttgac cgcgctaaca
    11761 ttttttctga tgaatcgagc caacagaaaa cgctgaaaaa acatccaaaa gatggaaaaa
    11821 ctcgcccgta acgaccagga ttatgattgc ccgcaaaagg cgggccagga caatccatag
    11881 ccgatatcca atcggaattt acgggagcat agtaatgaca gatattgcgc agttgcttgg
    11941 caaagacgcc gacaaccttt tacagcaccg ttgtatgaca attccttctg accagcttta
    12001 tctccccgga catgactacg tagaccgcgt aatgattgac aataatcgcc cgccagcggt
    12061 gttacgtaat atgcagacgt tgtacaacac cgggcgtctg gctggcacag gatatctttc
    12121 tattctgccg gttgaccagg gcgttgagca ctctgccgga gcttcatttg ctgctaaccc
    12181 gctctacttt gacccgaaaa acattgttga actggcgatc gaagcgggct gtaactgtgt
    12241 ggcgtcaact tacggcgtgt tggcgtcggt atcgcggcgc tatgcgcatc gcattccatt
    12301 tctcgtcaaa cttaatcaca acgagacgct aagttacccg aatacctacg atcaaacgct
    12361 gtatgccagc gtggagcagg ccttcaacat gggcgcggtg gcggttggta cgactatcta
    12421 ttttggctcg gaagagtcac gtcgccagat tgaagaaatt tctgcggcct ttgaacgcgc
    12481 gcacgagctg ggcatggtga ccgtgctgtg ggcctatttg cgaaactccg cctttaagaa
    12541 agatggcgtt gattaccatg tttccgccga cctgaccggt caggcaaacc atctggcggc
    12601 aaccatcggt gcagatatcg tcaaacaaaa aatggcggaa aataacggcg gctataaagc
    12661 aattaattac ggttacaccg acgatcgtgt ttatagcaaa ttgaccagcg aaaacccgat
    12721 tgatctggtg cgttatcagt tagctaactg ctatatgggc cgggctgggt tgataaactc
    12781 cggcggtgct gcgggcggtg aaactgacct cagtgatgca gtgcgtactg cggttatcaa
    12841 caaacgcgct ggcggaatgg ggctgattct tggacgtaaa gcgttcaaga aatcgatggc
    12901 tgacggcgtg aaactgatta acgccgtgca ggacgtttat ctcgatagca aaattactat
    12961 tgcctgatac gcctcatacg aacagcacca ccccgtagga cggataaggc gtttacactg
    13021 catccggcaa cctatgcctg atgcgacgcg gaagcgtctt atcatgccta catagcactg
    13081 ccacgtatgt cggaaaaggc gtttacaccg catccggcat aaaaacacgc gcactttgct
    13141 acggcttccc tatcgggagg ccgttttttt gcctttcact cctcgaacag ttttcatatt
    13201 gtcgttttgt gatcgttatc tcgacattta aaaacaaatc atttcattat attttgaaat
    13261 cgaaaacaag cgacaggata tgaaaatgta cgtggtatcg acaaagcaga tgctgaacaa
    13321 cgcacagcgc ggcggttatg cggttccggc attcaatatt cacaatctcg aaacgatgca
    13381 agtggtggtg gaaaccgctg ccaacctgca tgcgccggtc atcatcgccg gaacgcctgg
    13441 cacattcact tatgctggta cagaaaatct gttggcgctg gtcagcgcga tggcgaagca
    13501 atatcaccat ccactggcaa ttcatctcga ccatcacacg aaatttgacg atatcgctca
    13561 gaaggttcgt tctggcgtgc gctcagtcat gattgacgcc tcgcatttgc cttttgcgca
    13621 aaatatttca cgggtcaaag aggtggtgga tttttgccat cgctttgatg tcagcgtcga
    13681 agcggagctg gggcaacttg gcggccagga agatgatgtg caagtcaatg aagccgatgt
    13741 tttttatacc aaccccgctc aggcgcgtga atttgccgag gcaaccggaa ttgattccct
    13801 ggcggtcgcc atcggcacgg ctcatgggat gtatgccagc gcaccggcgc ttgatttttc
    13861 tcgactggag aacattcgcc agtgggtgaa cttaccgctg gtgctgcatg gcgcgtcagg
    13921 gttatcgact aaggatattc agcaaaccat caaactgggg atatgcaaaa tcaacgttgc
    13981 aacggagctg aaaaatgcct tctcgcaggc gttaaaaaat tacctgaccg agcaccctga
    14041 agcgaccgat ccccgggatt atttgcagtc ggctaaatcc gcaatgcgcg atgtggtgag
    14101 caaagtgatt gccgattgtg gctgcgaggg cagggcataa cgcacctgcc atttaacaag
    14161 gaaaaaacat gaaaacgtta attgcccggc ataaagctgg tgaacatatc ggcatatgtt
    14221 cagtctgttc tgcccatccg ttggttatcg aagcggcgct ggcatttgat cgcaacagca
    14281 cgcgcaaagt gctgattgaa gcaacgtcaa accaggtcaa tcaatttggc ggttataccg
    14341 gaatgacacc ggcagacttt cgcgaatttg tttttacgat tgccgataaa gtcggatttg
    14401 cacgcgaacg cattattctc ggcggcgatc atctggggcc aaactgctgg cagcaagaaa
    14461 atgcggatgc ggcgatggaa aaatccgtcg agctggtaaa agcatatgtt cgtgctggct
    14521 tcagtaaaat tcatcttgat gcgtcaatgt cctgcgcggg ggatcccata ccgttagcac
    14581 cagaaacggt tgcggaacga gctgctttgc tttgctgcgg aaagtgtggc gacagattgc
    14641 cagcgtgagc aactgagcta tgtcattggc actgaagttc cggttccggg cggtgaggcc
    14701 agcgccattc agtcagtaca catcacccat gttgaagatg ccgccaatac tttacgtacg
    14761 catcaaaagg cctttattgc ccgtgggctg acagaggcgt taacacgcgt gattgccatc
    14821 gtggtgcagc cgggcgtgga atttgatcac agcaatatta tccattatca gccgcaggaa
    14881 gcgcagccgc tggcgcaatg gatagaaaac acccgaatgg tttatgaagc acattctacc
    14941 gattaccaga cccggacggc ttattgggaa ttagtccgcg atcactttgc aatattgaaa
    15001 gtcggtcccg cattaacctt tgctttacgc gaggcgatat ttgcactggc acaaattgag
    15061 caggaactta tcgcccctga aaatcgcagc ggttgcctgg cggtaattga agaagtgatg
    15121 ctcgacgaac cgcaatactg gaaaaaatat tatcgtacgg gttttaacga ttcattactg
    15181 gatattcgtt acagcctgtc ggatcgtatt cgttattact ggccgcatag tcggattaaa
    15241 aatagcgtcg aaacgatgat ggtgaatctt gaaggcgtgg acatcccact gggcatgatt
    15301 agtcagtatc ttcccaaaca atttgaacgc attcagtccg gggaattatc agcaataccg
    15361 catcagctga ttatggataa aatttatgat gttttgcgcg cctatcgcta cggctgtgcg
    15421 gaataaggac ggtatatgac taacctgttt gttcgtagcg gaatttcttt tgtcgatcgt
    15481 agcgaagttt taacccatat cggtaatgag atgctcgcca aaggtgtggt tcatgatacc
    15541 tggccacagg cattaattgc cagagaagca gaattcccta ccgggataat gcttgagcag
    15601 cacgccattg caataccgca ttgtgaggcg attcatgcta agtcgtcagc catttatctg
    15661 ttaaggccaa caaataaagt tcattttcag caagcggatg atgataacga cgtggaggta
    15721 tcgttggtta ttgcgttgat tgtggagaat ccgcagcagc aattgaaact tttacgctgt
    15781 ttatttggca agttacaaca gcccgatatc gtcgagacac taatcactct tcctgaaacc
    15841 cagttaaagg aatacttcac aaagtatgtt ttagattcag acgaataaat ccctctgtaa
    15901 caataaaaag gactatttat gaaacgcaag attattgtcg cttgcggagg cgcggttgcg
    15961 acctctacga tggcggcgga agaaattaaa gagttgtgtc agaatcatag tattcctgtt
    16021 gaattaatcc agtgtcgggt taatgaaata gaaacctata tggatggtgt gcatttgata
    16081 tgcaccactg ccaaagtgga tcgtagtttt ggcgatattc cgttagttca cggcatgcct
    16141 tttatttctg gtgtcggtat cgaagcatta caaaataaaa ttctgactat cttacagggg
    16201 tgacctatgt tttcagaagt catgcgttat attctcgacc tcggccctac ggtgatgctt
    16261 ccgattgtca tcattatttt ttctaaaata ttaggcatga aggcaggcga ttgctttaaa
    16321 gcgggtctgc atatcgggat tggctttgtt ggcattggcc ttgtgattgg cttaatgctg
    16381 gattccattg gtccggcggc gaaagcgatg gcggaaaatt tcgacctgaa tctgcatgtg
    16441 gtcgatgtcg gctggccggg ctcttcacca atgacctggg cgtcgcaaat tgcgctggtg
    16501 gcgattccga ttgcgattct ggttaacgtg gcgatgttac tgacccgtat gacgcgggtg
    16561 gtaaatgttg atatctggaa tatctggcat atgaccttca ccggcgcgtt gctgcatctg
    16621 gcaaccggtt catggatgat agggatggca ggtgtggtaa ttcacgcggc gtttgtttat
    16681 aagctcggcg actggtttgc ccgcgatacc cgaaatttct ttgagctgga aggtattgct
    16741 attccgcacg gtacgtcggc gtatatgggg ccgattgcgg tgctggtcga tgctatcatc
    16801 gagaaaatcc caggcgttaa ccgaattaaa tttagcgccg acgatattca gcgcaaattt
    16861 ggtccatttg gcgagcctgt caccgtgggt tttgtgatgg ggctgattat cggcatcctc
    16921 gcgggttacg atgtcaaagg tgtattgcag ctggcggtaa aaacggcggc agttatgctg
    16981 ctgatgccac gggtgattaa acccatcatg gatggtttaa cgcccatcgc taagcaggcc
    17041 cgtagtcgtt tacaggcgaa gttcggcggt caggagttcc tgattggcct ggatcccgca
    17101 ttactgctgg ggcatacggc ggtggtatcg gcaagcctga tttttatccc gctcaccatt
    17161 ttaattgctg tttgtgttcc gggtaatcag gtactgccgt ttggcgatct tgccaccatc
    17221 ggcttcttcg tggcgatggc ggtcgcggtg catcgtggaa atctgttccg caccttaatc
    17281 tcgggtgtca tcattatgag catcaccctg tggatcgcga cgcaaactat tggtttgcac
    17341 acccaactgg cggctaatgc tggggcgtta aaagccgggg gtatggtggc ttcaatggat
    17401 cagggcggtt ctcccattac ctggttactg attcaggttt tctccacgca aaatattccc
    17461 ggtttcatta ttatcggcgc aatttatctt accggtattt tcatgacctg gcgtagagcg
    17521 cgtggtttta ttaaacaaga gaaaaccgtt ctcgcagaat aatttttacc tgagggggag
    17581 gttatccccc ttaacaatca ggagttttta tgaaatcaat ggtgaatgat actgatggta
    17641 tcgtgcgcgt tgcagaaagc gtcattcctg aaattaaaca tcaggatgag gtgcgggtaa
    17701 aaattgccag ctcgggctta tgtggttccg atttacccag gatatttaaa aatggtgcac
    17761 attattatcc aataacgtta ggccatgaat ttagcggcta tattgatgcg gtgggatccg
    17821 gtgttgatga tttacaccct ggcgatgcgg ttgcctgtgt gccgttatta ccctgtttta
    17881 cttatccaga gtgtttgaaa gggttttatt cccagtgcgc aaaatatgat tttattggct
    17941 cgcggcgtga tggtggattt gctgaatata ttgtcgttaa gcgaaaaaat gtctttgctc
    18001 tacccacgga tatgcctatt gaggatgggg cttttattga gccgattacc gttggcctgc
    18061 atgcttttca tttagcgcaa ggttgtgaga ataaaaacgt tattattatt ggtgccggaa
    18121 ccattggcct gctggctatt cagtgcgctg tcgcgctggg agcaaagagt gtgacggcga
    18181 tcgacattag ctcagaaaaa ctggcattgg caaaatcttt cggtgcgatg caaacattta
    18241 acagtagcga aatgagcgcg ccgcaaatgc agagcgtttt acgcgaactg cgctttaatc
    18301 agcttatcct cgagacggct ggcgtaccgc aaactgtcga actggcggta gagattgccg
    18361 gtcctcatgc ccaactggcg ctggtgggca cgttgcatca ggatctgcat ttaacatcgg
    18421 caacgtttgg caaaatattg cgtaaagagc tgacggttat cggcagttgg atgaactatt
    18481 ccagcccttg gccggggcag gagtgggaaa cggcgagccg gttgctgaca gaacgtaagt
    18541 taagcctgga gccattaatc gctcaccgtg gaagctttga aagcttcacc caggcggtgc
    18601 gtgacatcgc tcgtaatgcc atgccgggca aagtgttgct cattccatga aaccgcgggc
    18661 cagcgtgatg ctggcccggt attgtgcaaa acagatcatt caccaatggt cccccttcgt
    18721 ttacactagc cacaattgaa tatgggtaaa tgacgatgaa ttcattcgag cgaaggaata
    18781 agatcatcca attagtgaat gaacagggaa ccgtgcttgt tcaggatctg gcgggagtat
    18841 ttgctgcctc ggaagcgaca atccgtgccg atttgcgctt tctcgaacaa aaaggcgtgg
    18901 ttacgcgctt tcatggcggt gcggcgaaaa taatgtccgg taatagtgaa accgagaccc
    18961 aggaagtcgg gtttaaagag cgatttcagc tcgccagcgc gccaaaaaac agaatagcgc
    19021 aggcggcagt caaaatgatc catgaaggga tgaccgttat tctcgacagc ggaagtacga
    19081 caatgcttat cgccgaagga ttaatgaccg ctaaaaatat cacggtgatt accaacagtc
    19141 tcccggcagc gtttgccctt tccgaaaata aagacattac tctggtcgtc tgtggtggca
    19201 cggtccgcca taaaacgcgc tcgatgcatg gttccattgc cgagcgttca ttgcaggata
    19261 ttaatgccga tttaatgttt gtcggtgctg atggtattga tgcggttaat ggtattacga
    19321 cctttaatga aggttattcg attagcggtg cgatggtcac cgccgctaat aaagtgattg
    19381 ccgttctcga ttcatcgaaa tttaaccgtc gcggttttaa tcaggtactg ccaatcgaaa
    19441 aaattgacat tattattacc gatgatgctg tatcagaggt ggataaactg gcattgcaga
    19501 agacacgggt aaaattaatt acggtgtagt attgttctgt atgccggata aggtatttac
    19561 accgcatccg gcatttcgga ttctccctgg ttcaagagag aatagtttga ttaacgcaat
    19621 aatagacaat ctggtggtaa tcgacaacgc aacgccgccg gaagtatttc aatatgaaag
    19681 ttttgtccgc tcaacggttc gccatcaaga ttaaaagtga tttcgtgtgg ggcttgtata
    19741 tcaaaccacg acgaagcgcc ttcgataata ttcgggttat cttcgtcaga ttttaaggtt
    19801 gatacgagag ccggaagaat ttcatcgccg gtaaaaatgc gcagttgcag caagccatcg
    19861 ttaattaacg cgttcggaca caattgctga ccgccaccgg cctgacgccc gttaccaata
    19921 ccaatgacca gggcgtcacc ttgccagtga aagttttcac cgcggatttc acaacggtcc
    19981 ggttgcagag tatccatgcg cattaagcca tgaatgatgt aagagacgcc acccagcgcg
    20041 gcttttaatt tttccggcgt ttctgtggta atacgcgtcc caaatccgcc tgtcgccata
    20101 ttaataaaac aggtttgttt gttgacctgc gccatatcta tcgcaatggc gttaccggca
    20161 attgccagtt tcagcgcctt atccagtgcc tcaggaatcc ctacactggt ggcaaaatca
    20221 ttggcggttc ctaatggcaa aattcccagc gcgggtatgc catccccctc acactgaatc
    20281 aacgccgtag aaacttcatt aatggtgcca tcgccaccac cggcaatcac cgttgcgacg
    20341 cccaacttcc gggcctcctc tacatatcgt gcggcatcgc ctttctccca ggtgacccgc
    20401 acatggatcg tcattccttc ctcacgcaac agcataattg cttcgcgcaa gggtagattg
    20461 tcagtacttt tgccattaag aatcagtaag ctggcgggaa attctgccat gatcgtatgt
    20521 gcctttatga ttggtctgta gatagtgtag agcagaaaac aaaaggtgga gtcagaacag
    20581 gatgaaagtc ggaggataaa aagtcagttc aagtatggag gattcagtgg ttaccaccag
    20641 gagcttgcaa tggggacagc gaatactaac ggctgccacc gataaatttc aaaaaagagc
    20701 atatacctaa tattcaacta aacagtggca tcttcaatat aatatattaa agcccccatg
    20761 gagttaccct gaagggcctc aatgtccgta attcctactt atgtaggaaa tgttgtacag
    20821 aacatttatt ataatcctat tcaattataa taatcatgcc attattatat ttaaacacta
    20881 gagagtgtcg ttggtattta atgggggaag gtgagatgaa aaagatagct gctatatcat
    20941 taattagtat ttttcttata tctggttgtg ctgtgcataa tgatgaaaca agtatcggta
    21001 aatttggtct tgcatataaa agtaatattc agcgtaaact cgataaccaa tactacaccg
    21061 aagccgaagc ttctttagct aggggcagaa tatctggtgc agaaaatata gtaaaaaatg
    21121 atgcagccca tttctgtgtt actcagggca aaaaaatgca gatagttgac ctgaagacag
    21181 aaggtgcagg attacatggc gtcgctcgtc tgacattcaa atgtggagag tgagaatatt
    21241 ttttggtaag catcaaacat gcgcgttctg gttgtgctta gccggaacct gtgcgagcac
    21301 gatgccgtta cgtgaaaggc atcgtgctat gaagggagat tctatcgatg tggtcaatgg
    21361 aagacggtga ccagggatag ggcttatgca taaaaaataa gcccgtgtaa gggagattac
    21421 acaggctaag gaggtggttc ctggtacagc tagcatttta tgggttatgt ttttcagcga
    21481 aacggatgat aaccttaata aatgcagctg tatgtgatcg gtttctaaga attttccatc
    21541 cgggaaaaat aatcgaaatt aatcacttac cgtgtgggtt acgcgtggtt tccccggaga
    21601 aattacgcat cagcagcgcg taatttagct caagatcctg cgggaccggg agccacacag
    21661 tataaccatc gcctggtgcg accggcatcg cttcaccttt ggcgttttcc atgtgctcaa
    21721 gggtaaagtt aatgttgcct tgcggcgtca tcagctcaag gctgtcgcca acggagaatt
    21781 tatttttcac cgctaccgct gcgaggtccc ccttgcgctc accggtaaac tcaccaacaa
    21841 actgctggcg gtcagaaact gaataaccgt attcgtagtt ctgataatcg tcgtgagtat
    21901 gacgacgcag gaaaccttcg gtatagccac gatgcgccag accttccaga gtttccagca
    21961 ggctggtatc gaacggtttg cccgcagcgg catcatcgat agctttacga taaacctgtg
    22021 cggtgcgtgc gcaatagtag aaagatttag tacggccttc gattttcagc gaatgcacgc
    22081 ccattttggt caggcgttct acatgggcga tggcgcgcag atctttcgag ttcatgatgt
    22141 aagtgccgtg ctcatcttca aacgcggtca tatactcgcc cggacgctgg gcttcttcga
    22201 tcataaacac tttgtcggtt ggcgcgccga tacctagcgt cggctcaaca ttttgcaccg
    22261 gaatcggctc gtacttgtgt acgatgttgc caacgtcatc ttctttccct tcctggacgt
    22321 tgtactccca gcggcaggcg ttggtgcagg taccctggtt cggatcgcgc ttgttgatat
    22381 agccagagag caggcagcga ccggagtagg ccatgcacag cgcaccgtga acgaagatct
    22441 caatctccat atccggcacc tgattgcgga tctcttcaat ctcttccagc gacagctcgc
    22501 gagagaggat cacgcgggtc aggcccattt gctgccagaa tttcaccgtc gcccagttta
    22561 cggcgttagc ctgcaccgag aggtggatcg gcatttcagg gaagtgctca cgcaccagca
    22621 taatcagccc tggatcggac ataatcagcg catccggccc catttccacc accggtttca
    22681 ggtcacggat aaaggttttc agcttggcgt tgtgcggtgc aatgttgacc acgacataaa
    22741 actttttccc cagcgcgtgg gcttcattga tgccgagctg aagattttcg tggttgaatt
    22801 cgttgttgcg cacacgcagg gagtaacgcg gctggcccgc ataaacagca tctgcgccat
    22861 aagcgaaagc gtaacgcata tttttcagcg ttcccgccgg ggaaaggagt tccggtttaa
    22921 acataattct ctcgttctga tgacaggtca gctccgcttc acctgatgaa gcggttaggg
    22981 gagtgccccc actttaaggg cgggcattgt agcgctgtgg ggggatctgg taaagcgccg
    23041 gacgccgaca acccacgccc ggcatcgtaa ttacgtattg tctttcaccg tctggcttgt
    23101 gccattggct ttaacggagg caattccggt ttcacgagat tgcgcggtgg cgtacatctg
    23161 gctggagccg ataatttgat gattggcggc tttcagattg aaatagaatt tgccgttact
    23221 tgccgttttt ttctcatagc gttcttcttg cgggctgttg ctacgcaccg acgcgatgcc
    23281 cttttccgca gaggcttttg atgtatacag ctcgctggtg aggatagtct caccattgcc
    23341 cgcttttagc acaaaccgga actgattatc actgctctta ctgagttcaa accaaccagc
    23401 cataatgact ccttgattga aattgcgtat cgaaaacagc atctaaaggt gctgtttacc
    23461 gattaaagta tgagcgattt tctgatgcct gccagggcgg agagataatg tcagttggta
    23521 atgactccaa cttattgat
//
LOCUS       DAESHI010000061        23493 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.061, whole genome shotgun sequence.
ACCESSION   DAESHI010000061 DAESHI010000000
VERSION     DAESHI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23493
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.061"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(268..885)
                     /gene="tdk"
                     /locus_tag="KW011_002478"
     CDS             complement(268..885)
                     /gene="tdk"
                     /locus_tag="KW011_002478"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBH7660786.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1490..1903
                     /gene="hns"
                     /locus_tag="KW011_002479"
     CDS             1490..1903
                     /gene="hns"
                     /locus_tag="KW011_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HBH7660787.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2047..2955)
                     /gene="galU"
                     /locus_tag="KW011_002480"
     CDS             complement(2047..2955)
                     /gene="galU"
                     /locus_tag="KW011_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBH7660788.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="KW011_002481"
     CDS             complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="KW011_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HBH7660789.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="KW011_002482"
     CDS             complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="KW011_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HBH7660790.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5280..5738
                     /locus_tag="KW011_002483"
     CDS             5280..5738
                     /locus_tag="KW011_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HBH7660791.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5788..6630
                     /gene="purU"
                     /locus_tag="KW011_002484"
     CDS             5788..6630
                     /gene="purU"
                     /locus_tag="KW011_002484"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HBH7660792.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6790..6874
                     /locus_tag="KW011_002485"
     tRNA            6790..6874
                     /locus_tag="KW011_002485"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6824..6826,aa:Tyr,seq:gta)
     gene            6922..7053
                     /locus_tag="KW011_002486"
     ncRNA           6922..7053
                     /ncRNA_class="other"
                     /locus_tag="KW011_002486"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7237..7914)
                     /gene="narI"
                     /locus_tag="KW011_002487"
     CDS             complement(7237..7914)
                     /gene="narI"
                     /locus_tag="KW011_002487"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBH7660793.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="KW011_002488"
     CDS             complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="KW011_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBH7660794.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8621..10159)
                     /gene="narH"
                     /locus_tag="KW011_002489"
     CDS             complement(8621..10159)
                     /gene="narH"
                     /locus_tag="KW011_002489"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBH7660795.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10156..13899)
                     /locus_tag="KW011_002490"
     CDS             complement(10156..13899)
                     /locus_tag="KW011_002490"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HBH7660796.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14292..15683)
                     /gene="narK"
                     /locus_tag="KW011_002491"
     CDS             complement(14292..15683)
                     /gene="narK"
                     /locus_tag="KW011_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HBH7660797.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16022..17818
                     /gene="narX"
                     /locus_tag="KW011_002492"
     CDS             16022..17818
                     /gene="narX"
                     /locus_tag="KW011_002492"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HBH7660798.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17811..18461
                     /gene="narL"
                     /locus_tag="KW011_002493"
     CDS             17811..18461
                     /gene="narL"
                     /locus_tag="KW011_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HBH7660799.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="KW011_002494"
     CDS             complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="KW011_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HBH7660800.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20041..20394
                     /gene="ychN"
                     /locus_tag="KW011_002495"
     CDS             20041..20394
                     /gene="ychN"
                     /locus_tag="KW011_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HBH7660801.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="KW011_002496"
     CDS             complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="KW011_002496"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HBH7660802.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="KW011_002497"
     CDS             complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="KW011_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HBH7660803.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21791..22891
                     /gene="chaA"
                     /locus_tag="KW011_002498"
     CDS             21791..22891
                     /gene="chaA"
                     /locus_tag="KW011_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HBH7660804.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23294..23401
                     /locus_tag="KW011_002499"
     CDS             23294..23401
                     /locus_tag="KW011_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBH7660805.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
BASE COUNT         5883 a         6145 c         5666 g         5799 t
ORIGIN      
        1 tctggggccg tttttttgtt tacccaaagc aacttttcca taaaccgaca gcattagcct
       61 tcatcatatt tgcgacgatg tataacgcct aaacacaggg atattgtact ttacaggtca
      121 caagtcaacg tcggtgctta agagccctgt gaggcgtata gcggcgttaa aaaactgccg
      181 agaagggtat atagcccgga agaagtgcgt aaaacgaact gacaggataa aagtgccctg
      241 ctcaccctgt cagtaaagaa attcttatta atcgtggcga tgcctttcct gaatagccgt
      301 taatgagccg acttgtaacg cctctttata gtgtttacgg catacagaaa cgtatcgttc
      361 attaccacca attaccacct gctcaccttc gttataaggt ctgcctgctt gatcaagacg
      421 cagcaccatg cttgctttac ggccacaaaa acagatggtt tttaattcaa ccagtttgtc
      481 ggaccatgcc agtaagtatt ggctgccaat aaataattca cctcgaaaat cggtacgtaa
      541 accataacaa agtacgggta tatcgagttg atcgacaacc tccgataatt catatacttg
      601 ttgtctggtt aaaaactggc attcatcaac cagtacgcaa tgaattgcct gctgttcatg
      661 ttccgcacga atctcatcaa ataatgatga attttggtta aataattttg caggcgatga
      721 caaacctata cgcgaactga ctttcccggc accaaagcga tcatcaattt ctgccgtata
      781 tacgacagtg cgcatgccgc gttcctggta attgtatgaa gattgcaaca atgctgtaga
      841 cttacccgca ttcattgcgg aatagtagaa atatagctgt gccatcagcc acaggccctc
      901 aatgatatga aataaagttg gctggagttt atcataattc gtcagttttt cagctcaaca
      961 gtatgcacag aagtgggtat atgcgttctc ccttacgaag ccttgcataa tccttctgag
     1021 ctatcattac aactgcctcg cttgttataa gcgggtaaat gactactggt aactattcac
     1081 aatctttaac ctgttgcgca agtaatagcc ctctgttgac ctccaggaga tagtgcaata
     1141 ctaagtccat gctcttattg cgactgttct acttttcatc attcgcttaa tagggaattc
     1201 tcgtaaacac aactaataca gaagactgaa aggtcgtcag cctacgataa tctccccata
     1261 aaatgtgaca tgaatcagga agttttaacc tcacgtgctg cgaaatcatc ggtgtaaata
     1321 gggctatatg ccgcgtcttt tctggctaat tttatgaaaa gatatttatt ggcggcacaa
     1381 aataaagaac aattttgaat tccttacatt cctggctatt gcacaactga atttaaggct
     1441 ctattattac ctcaacaaac caccccaata taagtttgag attactacaa tgagcgaagc
     1501 acttaaaatt ctgaacaaca tccgtactct tcgtgcgcag gcaagagaat gtacacttga
     1561 aacgctggaa gaaatgctgg aaaaattaga agttgttgtt aacgaacgtc gcgaagaaga
     1621 aagcgcggct gctgctgaag ttgaagagcg cactcgtaag ctgcagcaat atcgcgaaat
     1681 gctgatcgct gacggtattg acccgaacga actgctgaat agccttgctg ccgttaaatc
     1741 tggcaccaaa gctaaacgtg ctcagcgtcc ggcaaaatat agctacgttg acgaaaacgg
     1801 cgaaactaaa acctggactg gccaaggccg tactccagct gtaatcaaaa aagcaatgga
     1861 tgagcaaggt aaatccctcg acgatttcct gatcaagcaa taatcttttg tagattgcac
     1921 ttgcttaaaa tcccgccagc ggcgggattt tttattgtcc ggtttaagac aatttaataa
     1981 gaataagcta taaaaaaacg gcgtcgattg ctcaacgccg tttcgtggat aacaccgata
     2041 cggatgttac ttcttaatgc ccatctcttc ttcaagccag gctttaaatt ccgtgccaag
     2101 ggtgttatga cgaataccgt attcaacgaa ggcctgcatg taacctaatt tattaccgca
     2161 gtcatggctc ttccctttca tatgataggc ttccaccgtt tctttttcga tcagcatatc
     2221 aattgcgtcg gtgagctgaa tttcatcacc agctcccgga ggggtttttg ccagcaacgg
     2281 ccaaatatcc gcgctaagta cgtaacgacc cacaatagcg agattagacg gcgcaacatc
     2341 cgctttcggt ttttctacca caccaaccat cggtacgctt tcacccggcg ctaattcaac
     2401 gcctttgcaa tccacaacgc catatgcggt cacatcagca accggttcaa ccatgatctg
     2461 gctatgaccc gtttcatcaa agcggcggat catctctgcc aggttatcct gtgacaaatc
     2521 ggattcatat tcatccagaa taacatcagg caaaataaca gctaccggtt catcacccac
     2581 taccgggtga gcacacaata ccgcgtgtcc caggcctttc gccagaccct gacgaacttg
     2641 cataatagtc acgtgcggtg gacaaataga ctgcacttca tcaagcagtt gacgttttac
     2701 acgtttttcc agcattgctt ccagttcaaa actggtatca aagtggtttt caatagagtt
     2761 tttagatgag tgtgtaacca gcacaatttc agtaatgcca gccgcaatac attcattcac
     2821 gacgtattga attaatggct tatcgacaag tggcagcatc tctttcggga tggctttcgt
     2881 cgccggcaac atcctggttc ctaatcccgc aacggggata acggcttttt tgactttcgt
     2941 attaatggca gccatttaaa ttctcctgga ctgttcgtgt tttgaacgtg ttcatatttc
     3001 tgtatcgcat cccagtatat cagcaccccg aaggggctaa gttctgaaaa ggaggcgagt
     3061 tagcataatt aagacaatta cttatacgaa tggcgacgat agtaccacca gccgacatta
     3121 gcaggtaatg caaatttagc ccgcgttatc gtttgctcat tctgcagaca acatcaagcg
     3181 cagtcgacca ccggttcccc atatttggca ttgccaggca tcgcatcgct ggctcaattg
     3241 attcaaataa gcgttaccta aagtgcctaa cggaacacca ttactgattt gcacctggtg
     3301 ttcgccggta tttaacgtcg cattcagacc cgcagaaacc agaatgagat ttttcagttc
     3361 gcgatgataa tagccaacta atagcggaaa ctgccccggc agattggcct gacggagtaa
     3421 atggtttacc tgcttcaata acgcgcccaa ctctggcaac cgttgatttt ggtgtgcaag
     3481 ctgttcctgt aataatccgt taaacaatgc gcgtaataac aaggcagcaa gtacgccatt
     3541 atgtccagct cgggtgacat caaggcaata aaatgccaga tcgttttccg aaagtgcggc
     3601 aatatcaagc accaggccgg gtttgtccgc ggcaaccaat tgacgataat taacccggca
     3661 atgggaaatc acctgctgaa ccggcggttg tagttcctgt aataatttcg ccgctgcggc
     3721 agggttatca accattgcat cccagtcgcg aaaaagcctt tcctcttcct caacgcgcga
     3781 attaaacatg ctgggataga gacaggcaaa aaccatctcg cgcaagcgat tcagatcttt
     3841 aactggtttc agcaaaacat cttcaacgcc cagacgtaac gctttggcaa tatctgccat
     3901 attttcagtg gcagatatca ccagaactgg ggtctggtcg cctctgttac gtatatgctc
     3961 cagcagttta agcccgttca ttcgtggcat cgcgatatca catatcatca ggtctggagt
     4021 gaaacctccc agcaactcaa gggcatccac cccatcagcc gccagtaccg ttgtcgctcc
     4081 caatgaggaa aaccatgaat ccagaagcga gcgaaatacc tgctcatctt caacaatgag
     4141 aatctgtttt ccgaccaatg gctgcgtcat gttctctccc ctgactggct ttactcaata
     4201 gtggcatgct attgccacct gcgcctgtca gaattaactt aagtgtagat aaaaattctg
     4261 gtcaaatgtt ggtgcgtacc aacggcaaaa gttcgtccat tttcctttcc actgccagct
     4321 gtccggccgc aatggcagcg tgcgcgcgat ggaaatcaag cgtagatatt tgcgggcaaa
     4381 caggttgaat cagaatatcg ggcggatcac ctgccatgcg gttcctttta aggcggttct
     4441 ccaacacctg gatagacgtg gtcataatct ctgttgccgt tggcgctgtc acagcccgac
     4501 gtgtcgttat gctgcctaac ctttctttca gacgcgcatg ccacggtaga gaatcaccat
     4561 tctcgctatt ttcttcactg acattaaagg agagcagatc ttgttgcatc aaatgagcat
     4621 cgtgctgcag gtcgaccgct atcacaatat cagcccccaa tgcacgcgta agagaaatag
     4681 gaattgggtt aactactgcc ccatcaacca gccagtagcc gttatgtgca acgggtgcca
     4741 tgagtcctgg aatactgcat gatgcgcgaa tagcaagatg gagatcgcct tcagtaaacc
     4801 ataattcacg ccccgtactt aaattggtgg caacagccgc aaagcgacgg gaacaatttt
     4861 cgatctctgt ttccggcatt atttcgcgat attgattgaa gacacgctcg ccgcgcagta
     4921 acccaccgcg ctgccaggag aggtccatca ggcgtaaaac gtcccaataa ctgaaagagg
     4981 ttacccaatc ttccagcgca gataatcgat cgcatgcata ggccgcgccc accagcgaac
     5041 caattgaaca tcctgcaacg atatcaattt caatacccac tttttttagc gcattaataa
     5101 cgccaatatg cgaccaacct ctcgccgcgc cagatcccag cgccagccct atttttatct
     5161 ttctcattat tcccgcaaga ttcccctgga atgcaatcgt agccacatcg ctactgctcg
     5221 gttaacatag tgctactcag gcggttaata cgccgttatt gtttccaggg agattatttg
     5281 tgtctcagct ttgtccctgt ggtagtgctg tcgagtatag cctatgttgc cacccttatg
     5341 tgtctggtga aaaggttgca cctgatccag aacatctcat gcgttcgcgt tactgcgctt
     5401 ttgtgatgca agacgcagat tatttaataa agacctggca tccctcttgt ggagcagcgg
     5461 cgttacgtgc cgaattgatg gccggatttg cgcatactga atggttggga ttgaccgtat
     5521 ttgaacattg ttggcaggac gccgacaata tcggatttgt cagttttgtt gctcgattta
     5581 ccgaaggtgg caaaacaggt gcaataattg aacgttcgcg atttttgaaa gaaaacggtc
     5641 aatggtacta tattgacggt acacgtccgc agtttggtcg caacgatccc tgcccttgtg
     5701 gttcaggtaa aaaatttaaa aagtgctgcg gccaataatg gttgacggta cggtttagca
     5761 aacactctca acaaggtttt tccagcaatg cattcactcc aacgtaaagt tctgcgtact
     5821 atttgtccgg accaaaaagg tctgatcgca cgtattacca atatttgcta caagcacgag
     5881 ttaaatatcg tacagaacaa tgaatttgtt gatcaccgta ccgggcgctt ttttatgcgc
     5941 acggaactgg aagggatttt taatgattcc accctgctgg cggatctcga tagcgcattg
     6001 ccagaaggct ccgtgcgtga gctgaatcct gccggtcgtc gccggatagt gattctggtc
     6061 actaaagaag cgcattgcct tggcgatttg ttgatgaaag ccaattacgg cggcctggat
     6121 gtcgaaatcg cggcagttat tggtaaccac gatactttac gttctctggt tgagcgtttt
     6181 gatattccgt ttgagctggt aagccatgaa gggttaagcc gcaacgagca cgatcaaaag
     6241 atggcggaag ccattgatgc ttatcaacct gactatgtgg tgctggcgaa gtatatgcgg
     6301 gtattaacgc cggaatttgt ggcacgcttc ccgaataaaa tcatcaatat tcaccattca
     6361 ttcctgcctg cgtttatcgg cgcacgtcct tatcaccagg cctatgaacg tggtgtgaag
     6421 attattggcg caaccgctca ctatgtgaat gacaatctgg acgaaggccc aatcatcatg
     6481 caggacgtta ttcatgtcga tcatacctac acagctgaag atatgatgcg cgcaggtcgt
     6541 gacgtcgaga aaaacgtctt aagtcgtgcg ctatacaaag tactggcaca gcgcgtcttt
     6601 gtttacggta atcggacgat tattctttaa tcgccagcaa aaataactgg ttacctttaa
     6661 tccgttacgg atgaaaatta cgcaaccagt tcatttttct caacgtaaca ctttacagcg
     6721 gcgcgtcatt tgatatgatg cgccccgctt cccgataagg gagcaggcca gtaaaaagca
     6781 ttaccccgtg gtggggttcc cgagcggcca aagggagcag actgtaaatc tgccgtcatc
     6841 gacttcgaag gttcgaatcc ttcccccacc accatcactt ttcaaaagtc cgtaaactct
     6901 caaacgaatc cgcaattaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa
     6961 ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc
     7021 gcgcagcgct gagtaatcct tcccccacca ccatcgcttc cttaaataat caaaacctgt
     7081 tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct
     7141 gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac
     7201 cccgccgaag cagggtctat gtgaactaaa attcgcttag tgacgagcgc gcaccagctg
     7261 gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg
     7321 gaacagcagg aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc
     7381 atcgaggtgt tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag
     7441 tttcatcatc tcgctaccgt ccatatgctg agcggagaac ggaatggtca acaggcccag
     7501 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg
     7561 cacgcgtggg ctgaacagac gacgcttcag caacagcacg ccgccaatca gacacagcac
     7621 gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc
     7681 ttcatacatc cagtgcggcg tcagcatacc gaagaagtga ccaacaaaaa tccccagaat
     7741 accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga
     7801 cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     7861 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     7921 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     7981 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     8041 tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc
     8101 gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg
     8161 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata
     8221 tctttcaaac cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc
     8281 ggcagatggt ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     8341 agcaggtcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     8401 aacagtgacg tggcgcggcc acggtcgaac agttcgctgt actgggcctg ggcatcgagc
     8461 ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac
     8521 agatttttcg acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     8581 tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt
     8641 tgctggtcac atcgatggca tcaatacgac ggctgttgaa cagattgaat ttggtatctg
     8701 aaccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     8761 gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca
     8821 gataacggta catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg
     8881 tatcaacttt accgtcaacg gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt
     8941 tcagtgcgcg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     9001 ccggaatacg caagctttcg acgtctggca gaatgccgtt gctacccagc tcacccgcgt
     9061 ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     9121 attctggatg cagcggcagc gccagtttcc attccattgc cattttataa accggcgact
     9181 gctgtgcggc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     9241 tcggatcgtt cggatcgagg aacacctcca gctgacgctg gtaaagatct ttctcgttct
     9301 cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     9361 tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc
     9421 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacgggc
     9481 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     9541 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc
     9601 agtgttcgca caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg
     9661 ccttctggat gttgtcgaag ttcttgtctt tcgccagttt gtcaaactca ccacccaggt
     9721 catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac
     9781 gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgatagt
     9841 caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcgaagattt
     9901 tacccagcaa catggcacgg ttacccatgc gcggctgcag tttgccgttg attttacgga
     9961 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tgacccggct
    10021 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
    10081 tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga
    10141 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
    10201 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
    10261 gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt
    10321 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
    10381 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
    10441 ctggctgaca accgcacggg cagtcagagc accgttgctg ttgaatactt caatccagtc
    10501 gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc
    10561 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
    10621 gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac
    10681 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
    10741 acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat
    10801 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga
    10861 ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga atatcgagga agcggatctt
    10921 ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc
    10981 agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagtgtca ggatcatctc
    11041 tgccgcatca attgcggtgt tcagcatcgg ctggcctttc gccggacctt ccgctttggt
    11101 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
    11161 gccattaccg attttctcca tcaacgggcc aatagaggta aagcgttcgt aagtcgccgg
    11221 ataatcgcgc tctacgacca taatgtgtgg cgcggtttta cctgggatca ggtcgcactc
    11281 gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcatgctg
    11341 gataggcagc gtgacgatgt cggtttcttt acccagatgg ccgacgcaca cttcggagaa
    11401 tttcttcgcg atggctttgt agatttccca gtcgcttttc gcttcccagg ccggatcgac
    11461 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
    11521 gtaccaagtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
    11581 gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc
    11641 ttccggcttc acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc
    11701 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
    11761 ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg
    11821 gatggaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc
    11881 cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc
    11941 catgcgttcc gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc
    12001 catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt
    12061 atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc
    12121 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
    12181 gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
    12241 catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg
    12301 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac
    12361 gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc
    12421 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
    12481 ggtgctcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg
    12541 cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta
    12601 cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
    12661 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
    12721 gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt
    12781 ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg
    12841 acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta
    12901 gcgacgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
    12961 tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
    13021 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt
    13081 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc
    13141 ccaggcgatg atgtagctgg agttatacca gtcagcagat tccggtacgt cagtttgctc
    13201 gccccaggtt tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca
    13261 agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc
    13321 cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt
    13381 agatgcggcg atcagttcgt tcacctcctg ccaggaagaa cgaacaaatc caccgcgtcc
    13441 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
    13501 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
    13561 catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg
    13621 gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg
    13681 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
    13741 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
    13801 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
    13861 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
    13921 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
    13981 agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag
    14041 ataccccttt aggataatta acagccacca cataacagac tgtaaattaa gacgttttat
    14101 acctgtagtt atttaaacta ttaagatttt tatgcagatg ggtattaagg agtattcccc
    14161 atgggtaaca tattaatggc tcttaatgaa atccggcgaa ataagccata taatatgagt
    14221 atgttgtaac taaatgacgc ataaaaaaag cgcgacatca tgccgcgcaa aggataatca
    14281 aatattaaga tttatttttt agaatgccga ccatataccg cccaggtaat cactacgcag
    14341 gcgatataga aaatcaaaaa taccttcatt gcgccgactg gcgaacccgt taatgccagc
    14401 gagctaccaa acgctttcgg gataaagaag ccaccaatag cgccaatcgc agagatgaaa
    14461 cccagcgccg ctgccgtgtc ggttgccgct tcacgcatcg cacgttcgtc agaaccccct
    14521 tctgctttca cgcgatccat tgtcagttta cggaagatca ctgaaatcat ctggaaagtg
    14581 gaaccactac ccagcccagc tgtcaggaac agcgccagga agaccgcgaa gaacgccatg
    14641 aagcttccgc cctgcccgtc agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc
    14701 atcagaataa agttcaccag cgtgacacga gttccgccca gacggtcaga taatgcacca
    14761 cctgcagaac gcgccagcgc accaataaac ggcccgaaga aagcgtattg cagaatctga
    14821 acatccggga actgcgtttt tgacagcatc gcaaagcccg cggagaagcc gataaaggag
    14881 ccgaaggttg ccagatacag caggctcata atccacagat gaccccgttt gagtaccggc
    14941 aactgctcct tgatggaggc tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca
    15001 atggtgaaga tggcaaggaa cggcacccat atccaggacg cattcgccag atacagctca
    15061 gtcccatccg gctgtttgac gccctggcta ccaaatactg cgaaaatcga cagtgatacc
    15121 accagcggag caaccaactg catgacgctg acgcccatgt ttcccagacc accattcaga
    15181 cccagcgcgc caccctgctt ctgtttcgga aagaagaagc tgatgtttgc catactggat
    15241 gcgaagttcg cgccagcaaa gccgcatagc agagagatga tgatgaagac gctataaggc
    15301 gtggaggtat cctgcacggc aaaacccagc cagacgcaag gaataatcag aataccggtg
    15361 ctgaacgccg tccagcgacg accaccgaag ataggaacca taaaggagta tggaacacgt
    15421 aataacgcgc cagaaaccga aggcagcgca gtcaacataa atagctgatc ggtcgtaaaa
    15481 ttaaaaccga ctttcggtaa gttcaccgca acagcgctga acaacatcca tacgcaaaac
    15541 gccagcagca gacagggaac ggaaatccac aggttacggc tggcaatacg ttgaccgcgt
    15601 tgttgccaga acgcaggatc ttccggtcgc caatctgtaa tgacagctcc agtagccctt
    15661 tcgggggcgg atgagtgact catagacacc tctgatactc gtttcgatgt ctgccacctt
    15721 agtgtctgta gctaaaggca atttgatgta aatcaaacga taagataact ttatctatca
    15781 ttgatattta tcattaccca tagtgagtac agtgatttca taaaaattat gagatttttc
    15841 acggtgctgt aaaatcccta cccttaccga tgtaaagcga ctaaccacac ggcaaataag
    15901 gagtaactct ttccgggtat gggtatactt cagccaatag ccgagaatac tgccattcca
    15961 gaatgtatcg tcacattcat taaggttatt gctcatttaa agcctgaagg aagaggtttg
    16021 catgcttaaa cgttgtctct ctccgctcac cctggttaat caggttgcgc ttattgtgtt
    16081 gctttctact gctattggac tggcagggat ggcggtttct ggctggctgg tgcaaggcgt
    16141 tcagggcagc gcccatgcga tcaacaaagc gggatcgctg cgcatgcaaa gttaccgtct
    16201 gttggcggca gtgccattaa gtgagaaaga caagccctta attaaagaga tggaacaaac
    16261 ggcatttagc gccgagttaa ctcgagcagc agaacgagac gaacaactgg cgcaattaca
    16321 gggtttacaa gattactggc gtaatgaact gatccctgca ctgatgcgtg cacaaaaccg
    16381 cgaaacggtg tcagcggatg tcagccagtt tgttgccggg cttgatcagc tggtatctgg
    16441 ttttgaccgc accacggaaa tgcgcattga gacagtggta ctggtccatc gggtaatggc
    16501 ggtatttatg gcacttttac tggtgttcac tattatctgg ttgcgggcgc gactgctaca
    16561 accgtggcgg caactgctgg caatggcgag tgccgtcagt catcgcgatt ttacccaacg
    16621 cgcgaacatc agcgggcgca acgaaatggc gatgcttgga actgcattga acaatatgtc
    16681 tgcagaactg gccgaaagtt atgccgtact tgagcagcgg gttcaggaga aaaccgccgg
    16741 gctggagcat aaaaatcaga tcctctcttt tttatggcag gctaaccgcc gtttgcattc
    16801 ccgcgccccg ctgtgtgaac gcctgtcacc tgtactcaac ggcttacaga atttaaccct
    16861 gctacgtgat atcgaattgc gggtgtatga cactgatgat gaagagaatc atcaggagtt
    16921 tacctgccag ccagatatga cttgtgatga taaaggctgc cagctctgcc cgcgcggcgt
    16981 attacccgtt ggtgatcgcg gcacgaccct gaagtggcgg ctggctgact ctcatacgca
    17041 gtacggtatt ttgctggcga ccctgccgca ggggcgtcat cttagccatg atcaacaaca
    17101 actgctggat accctggttg aacaactcac cgccacgctg gcgctggatc gccatcagga
    17161 acgtcagcaa cagttgatcg tgatggaaga gcgtgccacc attgcgcgcg aactgcatga
    17221 ttctattgcc caatctctct cttgcatgaa gatgcaggtg agttgtttac agatgcaggg
    17281 cgatgcgctg ccagaaagca gccgcgaact gttaagtcag atccgtaacg aactgaatgc
    17341 atcctgggcg cagttgcgtg aattgctcac cacattccgc ttgcagctca ccgagcctgg
    17401 attacgtccg gcgctggagg cgagttgcga agagtacagc gccaaatttg gcttcccggt
    17461 gaagctggat tatcaattgc cgcctcgcct ggtgccttcg catcaggcaa tccacttgtt
    17521 gcaaattgcc cgtgaggcat taagtaacgc cctcaaacat tcgcaagcga gtgaagtcgt
    17581 ggtgacggtg gcgcaaaacg ataatcaggt caaactgacc gtccaggata acggctgcgg
    17641 cgtgcctgaa aatgccatcc gcagcaatca ctacggcatg ataataatgc gcgaccgtgc
    17701 gcaaagttta cgaggcgatt gccgcgtccg ccgtcgtgaa tcaggtggca ccgaagtggt
    17761 ggtcaccttt attcccgaaa aaactttcac agacgtccaa ggagataccc atgagtaatc
    17821 aggaaccggc tactatcctg ctgattgacg atcacccgat gctgcgaact ggcgtaaaac
    17881 agcttatcag tatggcacca gatatcaccg tggttggcga agcgagtaat ggcgaacagg
    17941 gtattgaact ggcggaatct cttgatcccg atctgatcct gttagatctc aatatgcccg
    18001 gcatgaacgg tctggaaacg ctggataaac tgcgcgaaaa gtccctctca gggcgcattg
    18061 tggtattcag cgtctctaac catgaagaag atgtggtcac cgcactgaaa cgcggcgcgg
    18121 atggttatct gttaaaagat atggaaccgg aagatctgct gaaagcattg catcaggcag
    18181 ctgctggcga aatggtatta agcgaagcat taacgcctgt tctggccgcc agcttgcgcg
    18241 ctaaccgtgc cactactgag cgcgatgtta accagttaac cccacgcgag cgcgatattc
    18301 tcaagctgat tgcccagggt ttgccgaaca agatgattgc ccgccgcctg gatatcaccg
    18361 aaagcacagt aaaagtgcac gtcaagcaca tgctgaagaa aatgaagctc aagtctcgcg
    18421 tggaagcagc ggtatgggtg catcaggagc gcattttctg attacggttc ccagcgcagt
    18481 tcgtcgtttg acaatgcatc gaacggttcg acaagcgtta gcgtgatctc attggaggag
    18541 acacgctgcc cctggttatc ttccaccacc accgacaatc gccagtgatt gctcgcccct
    18601 tccccgttct gccagtcagg catgatcagc gtccagccct ccgcgctgtt ggcttgtgcg
    18661 cccggcgtca aacttaatat ctgcgtatcg ccctgccaaa tcagttgccg aataccgtaa
    18721 cgactgcgga tttgtaattt cagcggcact gtttcgccag gttttagatc ccacggcggt
    18781 gtcgccagaa acaccgttaa cgtttttcgc tgtcggtact caagagtcgg tagattattt
    18841 cgctgcggat tatcatagcg actaccacgt aacgactgac tttcggcaac ctcgcccgca
    18901 gaaagttgtt ttttgagcgg cacaccaaag cggtaattaa gattcagtcc gaggttattt
    18961 tgattttcgc cactttcacc ctgtttatgc tgggccgtca cagtgactaa tggcacaggg
    19021 gtgtaattta atcccagact caacgcgacg ggattgtgat aacccgtacc agagttaaac
    19081 aaatcaacac gctcaccaaa atactgttct accctgacac tggtattgag gtgttgatag
    19141 aacggcatgc gcatccgggc tgtcaggtca tacccgcgcg ccatccgttg ttcctgcgtg
    19201 gctgtctgtt catgccatgc agcaaacggc tgataaaagt ttgccgatag tcgcaaatat
    19261 tcgccccacg cttcggcacc aaagcccgct cgctgaagat tttcgtccag caagttgtca
    19321 taaaaagtgt tataacccac cagccagttg ccgcgcgccc agcgttgccc aacgcccaca
    19381 ttgctcacca acccatcatc ctgctgagta agaccaagct ggctccaggt gagataacga
    19441 tcattatctt gtaacggcac aaaccagctt ccacgactgc cggtgaaatg tccttcgtta
    19501 tccactttga cgtcaacact ggcatttccc cacggtgata gccaggactc tacgtgctga
    19561 ttaacctgtt gactaagcgc gtcgcggact tttcccaatg cgaaagcttt tgcctgctcg
    19621 ccagtatcca gcccgttatc attcatactg gtttcgccga aatctttcac aatttcagca
    19681 aagtgttttt ccccatcatg gttttcggga gccatgccta aatccggcag accatcattg
    19741 ttattatcaa agggatttgc cgctttttgc tcgaaggtag attgtgcgtt agctgtaccg
    19801 cctgccgcca gcaagagtaa ataaaacggg ataatgcgag gaacgaaacg gctcaaaacg
    19861 tcggtaactt ccggtaataa tgaaaaacca tagccttaaa gaataacgat tactggcaac
    19921 ttttgtcgta ttttctcgcg ctttcaggaa aatccttaaa aaggtgaccg attggtcaga
    19981 cgctgcagaa gcagtaagat agaagatgag tgtctaattc cctgaataca ggagtaaata
    20041 atgcaaaaaa tcgtgatcgt tgccaatggc gcaccttacg ggagcgaatc cttgtttaac
    20101 agcttgcggc tggccattgc gttacgagag caggagagca atctggatct gcgtctgttc
    20161 ctgatgtctg atgcggtcac agccgggttg cgcgggcaaa aaccagggga aggctacaac
    20221 attcagcaaa tgctggagat ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc
    20281 tgtaccgacg ggcgcgggat tagcacactt cctcttattg atggggtgga aatcggtact
    20341 cttgtggaac tggcgcaatg gacgctgtca gccgataaag tgctcacatt ttaatgtttg
    20401 agggggcgtc tgacgcccca ctatgaggga agtttactca ggcgaatccc ggccgtaaca
    20461 caccatccgg ataattctcc gccagcagtt tttttaccga taccagcaaa tcattcagcc
    20521 catcgtcctg cattcccagt ttgataagct cctgttccag tgaaaacagg tattgtgcgt
    20581 tggttcccag cggaccgctc gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt
    20641 cagattcata ttctggatgt cgcgggtcca taataaacac aatggcgttt actgtgcgtc
    20701 catcatcaag atcaagctga caccaggttg gcagataaca gccggtaatc atctcgcgct
    20761 tccacaacag ggttagttcc tgctccagcg tctcttctgg tagtcgatag gcgacgcctg
    20821 tggtgcgtcc gccctctttc agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc
    20881 cggcggtcag gcgcaggcaa aacgcgcgat gccatccaac cagtgtaccg gtgcataatt
    20941 cggtaaactc cagtgccgga ttccacatca acgatccata accgaagatc cacaccggtc
    21001 cctcattagg tcgacaagcc agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc
    21061 ataagagtga ttcttcaatg gcaccaaatg ccgttttaca atcggcattc atcaagaaat
    21121 cacgcgttat caccttacac ctccgccact gcatacttcc ctgcgacaac tttttggtgt
    21181 cttccggaca tttttaccgc tcattttgaa accactaacg ggacaatatg caatccacgg
    21241 accacttgca aggcaacacc gcacgaatag ctttaactaa gctaccggtt ttacgatttt
    21301 ttatgccatt tatcatcatc ccctttggca tattcatgct tcacagcagc ccaggccact
    21361 ttatgcgcgg tttcttcgcg actggcgtca tcacgccgat cttctttatc tttatattga
    21421 tcccatgcgc tgttgaacgc ttctttatag atatcctggg catgagacgg tagaacgtgc
    21481 tttacgcttt ccggcagatc gcttttcgtt ttatacggca tcgttgactc cttttacaaa
    21541 gttgaacatt aagtgtggtc aacaatatct actcttgcca aaacataaga attttcatgt
    21601 cagactgttc ttaagaaacg ctaaaacaaa cattgtctaa acgtaatact tatttttatg
    21661 ccccaatgag taaaaaatcg acagcggggt aaaaattcgt aaaatgttac cgatatccag
    21721 acactttctg ttatttttcc tacctatttt ttacattttc gataaattca tctgcaaagg
    21781 gataaccata atgtcaaatg ctcaagaggc ggtaaaaacc cgccacaagg agacttcgct
    21841 tattttcccg gttctggcgc tggtagtgct gttcctgtgg ggaagcagcc agacactacc
    21901 agtggtcatt gccatcaatc ttcttgcgct tattggtatt ttaagtagcg cctttagtgt
    21961 tgtccgtcat gcggacgtat tagcccatcg cctgggagaa ccttacggtt cgcttattct
    22021 tagcctttca gtggttattc ttgaagtcag tttgatttca gctttaatgg caaccggcga
    22081 cgccgcgcca acgctaatgc gtgatacgct ctattcaatc attatgattg ttaccggtgg
    22141 gctggttggc ttttcattat tgttgggcgg tcgtaagttt gccacccaat atatgaatct
    22201 gtttggtatc aagcagtatt taattgcgct gttccccctg gcgataatcg tactggtatt
    22261 tccaatggct ctgcctgcgg cgaatttttc aaccggtcag gcgttactgg tagcattaat
    22321 ttctgcggca atgtatggcg tatttttgct gatccagacc aaaacgcatc aaagtttgtt
    22381 tgtctacgag cacgaagatg acagtgatga cgacgacccg caccacggta aaccgtctgc
    22441 ccatagcagc gtgtggcatg ctatctggtt gattatccat ctgattgccg ttattgcagt
    22501 caccaaaatg aatgccagcc cgctggagac attgctcgac agcatgaatg cccctgtcgc
    22561 ctttactggc ttcctggtgg ctctgttgat tctgtcgccg gaaggtttag gtgcgttaaa
    22621 agcagtgttg aacaaccagg tccagcgcgc gatgaatctg ttctttggtt cagtgttagc
    22681 aaccatttcg cttaccgtac ctgtcgtcac gctaattgcc tttatgacgg gtaacgaatt
    22741 acagtttgca cttggtgcgc cagaaatggt ggtgatggtg gcctctttag tgctgtgcca
    22801 tatctccttc tccaccggac gtactaacgt gctcaatggc gcagcgcatc tggcactgtt
    22861 tgccgcctat ttgatgacga tatttgcctg acagttatat ctcctccgat gacggaaatc
    22921 gggggagata agataaattc ctgcttattg tatttcttca tactacctgg acagctcgtt
    22981 aatcagaatt gcgagcctgt accacaacct gcacgaaacc actgcaacag ctgttgaaaa
    23041 gccggaatgt actctttatt gcaaacattt cagggatgaa gtgaagatga attgagacgt
    23101 agtatccaca ccaagccgga attatccggt gaggcgcaat gttgcggggg ctttatccct
    23161 ggtggcattg gttgctggaa agagaaaacc cccgcacgtt gagaggtaaa acctgacaac
    23221 aaaacggggg ctaatcttga aaccagacac ttcaagaata gccgccttgt tgtcattaca
    23281 acaaggcggc tatatgacgc tcgcgcagtt tgccatgact ttctggcacg acctggcagc
    23341 accgatcctg gcggggatta ttaccgcagc gattgtcagc tggtggcgta accggaagta
    23401 aggtgaaaac gggcgtcagg ctgccgtgat ggcaatatgc gcccgaaacc ggccgcaggg
    23461 gtaacagcct gcggccattt ttgtctttat ctc
//
LOCUS       DAESHI010000062        23237 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.062, whole genome shotgun sequence.
ACCESSION   DAESHI010000062 DAESHI010000000
VERSION     DAESHI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23237
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.062"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(40..240)
                     /gene="glgS"
                     /locus_tag="KW011_002500"
     CDS             complement(40..240)
                     /gene="glgS"
                     /locus_tag="KW011_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HBH7660806.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            509..1138
                     /locus_tag="KW011_002501"
     CDS             509..1138
                     /locus_tag="KW011_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HBH7660807.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            1165..2826
                     /locus_tag="KW011_002502"
     CDS             1165..2826
                     /locus_tag="KW011_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBH7660808.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            3065..3124
                     /locus_tag="KW011_002503"
     CDS             3065..3124
                     /locus_tag="KW011_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBH7660809.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            3441..3500
                     /gene="ibsE"
                     /locus_tag="KW011_002504"
     CDS             3441..3500
                     /gene="ibsE"
                     /locus_tag="KW011_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HBH7660810.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3620..5053)
                     /gene="hldE"
                     /locus_tag="KW011_002505"
     CDS             complement(3620..5053)
                     /gene="hldE"
                     /locus_tag="KW011_002505"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HBH7660811.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(5101..7941)
                     /gene="glnE"
                     /locus_tag="KW011_002506"
     CDS             complement(5101..7941)
                     /gene="glnE"
                     /locus_tag="KW011_002506"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HBH7660812.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7964..9265)
                     /gene="ygiF"
                     /locus_tag="KW011_002507"
     CDS             complement(7964..9265)
                     /gene="ygiF"
                     /locus_tag="KW011_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HBH7660813.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9507..10127
                     /locus_tag="KW011_002508"
     CDS             9507..10127
                     /locus_tag="KW011_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBH7660814.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            10191..11429
                     /gene="cca"
                     /locus_tag="KW011_002509"
     CDS             10191..11429
                     /gene="cca"
                     /locus_tag="KW011_002509"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HBH7660815.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11592..12413)
                     /gene="bacA"
                     /locus_tag="KW011_002510"
     CDS             complement(11592..12413)
                     /gene="bacA"
                     /locus_tag="KW011_002510"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBH7660816.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12504..12872)
                     /gene="folB"
                     /locus_tag="KW011_002511"
     CDS             complement(12504..12872)
                     /gene="folB"
                     /locus_tag="KW011_002511"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HBH7660817.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            12977..13594
                     /gene="plsY"
                     /locus_tag="KW011_002512"
     CDS             12977..13594
                     /gene="plsY"
                     /locus_tag="KW011_002512"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBH7660818.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13607..14539)
                     /gene="ttdR"
                     /locus_tag="KW011_002513"
                     /gene_synonym="dan"
     CDS             complement(13607..14539)
                     /gene="ttdR"
                     /locus_tag="KW011_002513"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HBH7660819.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            14746..15657
                     /gene="ttdA"
                     /locus_tag="KW011_002514"
     CDS             14746..15657
                     /gene="ttdA"
                     /locus_tag="KW011_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HBH7660820.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15654..16259
                     /gene="ttdB"
                     /locus_tag="KW011_002515"
     CDS             15654..16259
                     /gene="ttdB"
                     /locus_tag="KW011_002515"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HBH7660821.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            16308..17771
                     /gene="ttdT"
                     /locus_tag="KW011_002516"
     CDS             16308..17771
                     /gene="ttdT"
                     /locus_tag="KW011_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HBH7660822.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17814..18827)
                     /gene="tsaD"
                     /locus_tag="KW011_002517"
     CDS             complement(17814..18827)
                     /gene="tsaD"
                     /locus_tag="KW011_002517"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBH7660823.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            19064..19279
                     /gene="rpsU"
                     /locus_tag="KW011_002518"
     CDS             19064..19279
                     /gene="rpsU"
                     /locus_tag="KW011_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBH7660824.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            19390..21135
                     /gene="dnaG"
                     /locus_tag="KW011_002519"
     CDS             19390..21135
                     /gene="dnaG"
                     /locus_tag="KW011_002519"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBH7660825.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            21330..23171
                     /gene="rpoD"
                     /locus_tag="KW011_002520"
     CDS             21330..23171
                     /gene="rpoD"
                     /locus_tag="KW011_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBH7660826.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5654 a         6331 c         5907 g         5345 t
ORIGIN      
        1 ctaatacaaa cactaaaacg ggccatcagg cccgttatat cagtgctcta actccagctc
       61 tcttgcctgc atcatctgtt ggcaatacca ggcataacgt gcgcaaaacc aggttctatg
      121 ttcttcatcc atgttaccag taacggcaag aatatcgacc gggcgacctt ctgcgtgcat
      181 tctggcaaaa ctacgcgcca ggaaatcgaa attatttaaa gaattaagac tatgatccat
      241 aagcacatcc tccggttatc gcataccttt taaggccata tgctttttct gatttcagta
      301 ttccccaatc agtgtagtgc ctttaaacat aacgtgcgta aatatattgt ccccgatcac
      361 actttttagt gaaaaataaa aaactatatt aaaaaacttt ctcttccaga acttctctaa
      421 catgtgttat tattaacaaa gataaatgta agcaaggaaa ctatcccaaa gtaaatattg
      481 cgtcactaaa tggacattgg agtgacatat gattttattc gccgactata atacccctta
      541 tcttttcgct atttcctttg tactgttgat cggcctttta gaaattttag cgcttatctg
      601 cggtcacatg ctctccggtg ctcttgatgc acatcttgac cattataatt ccataaccac
      661 aggtcatatt agccaggcac ttcattatct caatatcgga agattgccag ctctcgtcgt
      721 tctctgcttg ctggctggtt tttttggtct tattggcatc ctgttgcagc atgcctgcat
      781 catggtctgg caatcaccac tgtcgaacct gttcgtggtt cctgtcagtt tactgtttac
      841 gattattgcg gtgcattata ccggtaaaat agttgcgccc tggattccac gcgaccatag
      901 ttcagctatt acagaagaag aatatattgg tagcatggca ttaattaccg gtcatcaagc
      961 cacttcaggt aatccctgcg aaggaaaact caccgatcaa tttggccaaa tacattattt
     1021 actactggag cctgaagaag gaaaaatttt cactaaagga gtcaaagtgt taattatttg
     1081 ccgactttct gcaacgcgat atttggcgga aaacaatccc tggccacaaa tattgtaatt
     1141 tattaatcaa aaggaaattt aataatggat gatattgtta attctgtgcc ctcctggatg
     1201 tttaccgcga ttattgccgt atgcattctg tttattattg gaattatttt cgccaggctc
     1261 tatcgtcgcg cttcggcaga gcaagctttt gttcgtactg gtttaggtgg gcaaaaagtg
     1321 gtgatgagcg gtggcgcaat cgtgatgccg atctttcatg aaataatccc catcaatatg
     1381 aatactctga agctggaagt cagccgctca accattgata gcctgattac gaaagatcgt
     1441 atgcgtgtcg atgtagtagt cgctttcttt gtacgggtaa aaccgtctgt agaaggcatc
     1501 gccactgcgg cgcagacgtt ggggcaacgc acgctatcac cagaagactt acgtatgttg
     1561 gttgaagata aatttgtcga tgccctccgt gcaacagctg cacaaatgac catgcatgag
     1621 ttacaggata cccgcgagaa cttcgtgcag ggagtacaaa atactgtcgc agaagaccta
     1681 tcgaaaaacg gtcttgagct ggaaagcgtt tcacttacca actttaacca gacctcgaaa
     1741 gaacatttca atccgaacaa tgcctttgac gccgaaggtt taaccaaact gactcaggag
     1801 acggagcgcc gtcgtcgcga acgtaacgaa gttgaacagg atgtagaagt tgcggtgcgt
     1861 gagaaaaacc gtgatgcact ttcgcgcaag ttggagattg aacaacaaga agcgtttatg
     1921 acgcttgagc aggagcagca ggttaaaacc cgtactgccg aacagaatgc acgtattgcg
     1981 gcttttgaag ctgaacgtcg tcgtgaagca gagcagacgc gaattctggc tgaacgacaa
     2041 attcaggaaa cggagatcga gcgcgagcag gccgtccgct caagaaaggt tgaggctgaa
     2101 cgtgaagttc gtattaaaga gatcgaacag cagcaggtca ccgaaatcgc caaccagacg
     2161 aaatcgatcg ctattgccgc caaatcggaa caacagtccc aggcagaagc gcgtgctaac
     2221 cttgcactcg cagaagcagt aagtgcgcaa caaaacgtag aaaccactcg ccagaccgca
     2281 gaagccgatc gtgctaaaca agttgcccta atcgctgccg cgcaggatgc agaaaccaaa
     2341 gcggttgaac tgaccgtgcg ggcgaaagca gaaaaagaag ccgcagagat gcaggcggcg
     2401 gctatcgttg agttagccga agctacacgt aaaaagggtc tggcggaagc agaagcacaa
     2461 cgtgcgctga acgatgctat caacgtactt tctgatgaac aaaccagcct taaattcaaa
     2521 ctggccttgt tgcaggcgct acctgcggta atagagaaat ccgttgagcc gatgaagtcc
     2581 attgacggta tcaagattat tcaggtcgat ggtctgaatc gtggcagcgc tgcgggtgat
     2641 gcaaacacgg gtaatgtggg gggcggaaac ctagcggaac aagcattatc agccgctctc
     2701 tcttaccgca cacaggcacc gctgattgac tccttgctca atgaaattgg cgtttcaggc
     2761 ggctcactgg cggcattgac ttcaccctta acctcaacaa ctcccgtcgc cgaaaacgta
     2821 gaataaattt tcagctatga acgtccctgg cacaaaaagc cggggatttt ttatatctgc
     2881 gttccgctaa aaactgcaaa tgatcaggcc gttgcaacgt tttgcgtgac cgctcgggga
     2941 aggcaaaact gcctctggga aagcgttgcg cgaggtccgg cgctcatcaa caatcgggga
     3001 gtcagcaaag ggctgaaacg ggaaagcccc tcccgaggaa ggggccttac agaaggaaag
     3061 ggttatgatg aagctcgtca tcatactgat tgtgttgtta ctcgtaagtt tcgcagctta
     3121 ttaacagcca atcagagggg ggagaaatcc tccctcaccc ttgttccttt actttaggtt
     3181 gaaaaaaaat cagcgtcaag aaggtttcat taacacatat ccctggccca aaaagccggg
     3241 gattttttat atctgcgttc cgctaaaagg tgcaaatgct caggacgttg cagcgttttg
     3301 cgtgatcgct cggggaaggc aaaactgctt cagggaaagc gttgcgcggg gtccggcgct
     3361 catcaacaat cggggagtca gcaaggggct gaaacgggaa agcccctccc gaggaagggg
     3421 ccttatagaa ggaaagggtt atgatgaagt tcgtcatcat actggttgtg ttgttactgt
     3481 taagtttccc gacttactaa caactgatca gagggggaag aaatcctccc ttacccttgt
     3541 tcctttactt taggttgaaa aaacaacagc gtcaataggc ctgcctgcta caaagcgaga
     3601 tctgtgaacc gctttccggt tagccttttt tatcctgttg gatcttcttg atgatgttag
     3661 tggtcgagca accgtcttcg aagttgagca ccagcacttc gccgccgttg gcccagactt
     3721 ctttactccc ggcgatctct tctggtttat agtcgccgcc cttcaccagt aaatctggca
     3781 ggatcccggc gatcaagcgc tgcggcgtgt cttcttcaaa cgacaccacc cagtcgaccg
     3841 cttccagtgc gcccagcaca atcatacgct gttcgagcgg gttaacgggg cgggaatccc
     3901 ctttcagccg tttggtggag gcatcgctgt tcacagcgac aatcagacgg tcacccagtt
     3961 tgcgagcatt tgccagatag gagacgtgcc cggcgtgcag gatgtcaaag acaccgttgg
     4021 tcattaccac cttttcgccg cgtttacgcg ctgccgctac ggccagcttc agttcctctt
     4081 cggtcatcac gccaaagccg gtatctgcgc gcccacgcac tgcattttcc agctcgatcg
     4141 gcgaaaccgt ggaggttccc agtttgccga ccaccacgcc agccgccgca ttggcaaaga
     4201 agcaggcttc ttccagcgaa ttacccgctg ccagcgttgc cgccaggacg ccaatcaccg
     4261 tgtcgcccgc accggtaacg tcatacactt cctgcgcctg ggttggcata tgcagcggcg
     4321 ctttacccgg ttgcagcagc gacataccct gttcggaacg ggtcactaac agagccgaga
     4381 gttcgtaatc ggcaatcagt ttcatgccgc gctcaacaat ctcttcttcg gtcttacatt
     4441 taccgacaac agcttcaaat tccgagagat tcggcgttaa cagcgtagcg ccgcggtagc
     4501 gctcaaaatc ggtacctttt ggatcaatca gcaccggaac gcccgcttta cgcgccagtt
     4561 ggatcatctg ctgtacgctt gccagcgcac ctttggcgta gtcagaaagc accagcgcgc
     4621 caatcgaact cagcgcctga ttaatccgct cgtgcagcgg ctgcggatca acgccttcga
     4681 aaccttcttc gaaatccaga cggatcagct gttggttgcg ggaaagtacc cgtaatttgg
     4741 tgatggtcgg atgcgtcggt acagaaacga agtcgcattt gacgttgacg tcggccagag
     4801 atttactcag cgcgcgcgct gcatcgtcaa tgcccgtcaa cccgaccagg cgtgcattag
     4861 caccgagaga agcgatattc atcgccacgt tagccgcgcc gcccggacgt tcttcgatgg
     4921 tattcacttt aaccacgggc accggcgctt ccggcgagat acggctggag ggaccgtacc
     4981 agtaacgatc cagcattaca tcaccaacca ccatcactcc tgcacgttca aactctggca
     5041 gcgttacttt cattcctgtc tcctgagaga ttcaaaattt gcgcgcgata ataccatact
     5101 tcattcttcc accagccact tctgccagct tgcccgcacc agatcacgct ctgcggtgaa
     5161 gcaatccccc gacacatggc ccggcaattc ctgtaatgcc agatgatgaa gttcatcgcg
     5221 cagcgtcgtg taagcacggg tcagcgccat cgcttcctgc tcttccataa tgtcgttttg
     5281 cgccagcagc tccagaatac gcacgttgtc tgaccagcgc gttaacttcg gtttttcatg
     5341 agcgtagcgc aacaccagat attgggtaat aaattcgata tcggtaattc cgccttcatc
     5401 agctttgata tcaaagcgat cgcgatgttt attgccgaga tgagcgcgca ttttctcgcg
     5461 catttcccgc acttccgttt gcagagttct accttcacgc ggcagcgtca taatctcgcg
     5521 acgcactgcg tcaaagtgcg cggtgagctg cggatcgccg tacactaccc gcgcacgcac
     5581 cagcgcctga tgttcccacg tccaggcctc gtttttctga taatcggcaa atgcttctgt
     5641 ggaggtaacc agcattcccg ccgccccgga cgggcgcagc cgcgcatcca cttcgtacaa
     5701 aatgccggaa gaggttcgcg tgctgaacag gtgcataatg cgttgcgcca gacgaagata
     5761 aaactgtcgt ccgtcgattt cccgctcgcc gtcagtcatc gcatccattg ggcaatcatg
     5821 gaggaagatc aggtcaagat cggaactgta gcctaactcc cagccgccca gcttgccgta
     5881 gccgaccact gcaaaaccac gcccttcgcg atcgttgagg tgattcggct taccgtagcg
     5941 ggcaaccatt tgaacccacg cctgctgaac gacggcatct atcatggctt ccgccagcca
     6001 ggttaagtga tcgctcactt tcatcaccgg tagcgtacca gcgatatccg ctgcggcgat
     6061 gcgtaacagc tgcgcctgtt tgaactgacg cagcgcctca agctgttgct cttcgtcatc
     6121 ttccggcacg cgcagcaaat actggcgcaa ctcatcgcgg taggcatcgg tcgccgtcgg
     6181 ctggtaaaga gtgtttggat cgagcaattc atccagcaat aatggataac gcgccagctg
     6241 gctggcaatc atcggcgatg cggcacacag agaaatcaaa tggttaagcg ccgcagggaa
     6301 ttcactgagc aattctaaat aggtggtgcg ggtaacaatc cccaccagca aggcggtaat
     6361 gcgcgacagc gtgacagcag cgtcttcacg cgcacagaca tcacttagca gatgcggcat
     6421 cagatggtcg agcacctgac gtcctcgcgg cccgatggtg cgcttatcca gctctttgcg
     6481 gaaatcggaa atcagcgtta gcacctgttt gcgatcatcc tcgctaagat gcgccagcac
     6541 tggcgtagtg tcatcttcct gcaacgcatc ctgccacagc tcacgccact gttccgacag
     6601 cgactcttcc tgagtttcac tttcgtcgtc gccaatcaat tcattaaaca cccggcgcac
     6661 attgttcata tgtgcggtca gcgccccggt cagttgcggc cagtcagcaa agtccatcgc
     6721 ccacgccagc cgcgcacggt taagctcatc agaaggaagc gtctgggttt gttcgtcgtt
     6781 aatgctttgc agcaggtttt ccagacgccg caggaacaga tacgccactc gcaattgttc
     6841 agcatcgttt tcagaaagca gatgcagcgc agcaatggcg ctgagcgttg gcagtaaaga
     6901 gcacgattgc agcgacggtt cgcgcccgcc gcgaatgagc tggaatacct ggacgataaa
     6961 ttcaatttcg cgaataccac ccgcgccaag tttgatgttg tcggtcaaac cgcgccgacg
     7021 cacttcacgg gcaatcattc ctttcatgtt gcgcagcgac tgaatcacgc tgaaatcgat
     7081 gtaacgacgg aaaacaaacg ggcgtagcat cgcacgcaac tcgttagcat agacgccttc
     7141 gctatcgccc ataatccgcg ctttgaccat cgcgtaacgc tcccagtcgc gcccctgctc
     7201 ctggtaataa tcctccaacg cggcaaagct cagcaccagc gggccacttt cgccaaacgg
     7261 acgcagtcgc atatccacgc gatagacgaa gccatcctgc gttggttgat ccagcacttt
     7321 aatcagccgc tgccccatgc gggtaaaaaa ctgcgcgtta tccagttccc ggcgtccgcc
     7381 ctgcgtacaa ccatgttccg gccaggcaaa aatcagatcg atatcagagg agaaattcag
     7441 ttccccgccg cccagcttac ccatgcctaa gatcagcagc ggttgcgctt cgccctgcgc
     7501 attgcacggc gttccccact cgcggcagca ggcgtcatac agccagtcac gcgccgcaac
     7561 aatcagcgtc tccgccagat aactgagctg ctgcaatatg ctctcttcag taaccagtgc
     7621 cagcgtttgc gcccatgcga tgcgcaccat aatgcgccgc cggaatagcc gcagctcgcg
     7681 cattaacccg gcttcgtcac tcacattact gagcgcctcc tgcaaccatg cagcgtaatg
     7741 ctgccattca tcggcctgcg gcggttggct ttccagttcc gtcaaccact ctcgatgcgc
     7801 aattatgctg tcctgcacaa aatcactgaa agtaagtact gacttcgcct gtgcacttag
     7861 tggttcctcg gctaaaggct ctggcagccg ctcaacaacg gtctgccagt actgctgtaa
     7921 cggtgaagag agcggcttca taagcgattt tatccttgcc tgattaacgt tttccgctgt
     7981 gcaaccagaa cggttcctga ttgtttgcct cattacggaa atgctcaatt tcaatgcgtt
     8041 gcccggtcgc aatagcgtga cgcagcccct gccagttctc cagccaggca tgcgcgacga
     8101 caggatcata gtaacccgcc agcaacagta ttgagtcaat gtcacgcgtc aggcgtggca
     8161 actggtcacg gtagagatcg cctaacggct ggcagaaaac gcttttcagt tcagcggcat
     8221 ggtgggaaag atggatatcg gcaaagcgtt tgaaggagtc gctgattttg ccctgcgctt
     8281 tggcatctaa aaatggctgc catgctttgc ttaccaacca ttcggtcaac gccagcttcg
     8341 ccattgccgt ttcggtagag tagaccgccg tcacggcaga aaccgcagaa gcaatggtcg
     8401 cttcgcattg ggtcagcaaa tcacgtaagt gagtgctcgc tttacgcggc acaataccac
     8461 cgaacagcat cagcgtatga cggaccaggc taatggctgc cagcacctgt tctttcgccg
     8521 catcgttacc gcgtacccac agttcttcat gatactgcca ttgcgctaac gccagctcga
     8581 gcgccgcttc cagcccctgt tctacatcgg cttttgccgc aacatgcaaa atggtggtcg
     8641 gtttgatttc acgcgccgga ttgccctgcg ccagatgata gccacgcgcc gctttgctca
     8701 ggctgccctg gcgtaatccg gtttgcgata ccagttggtt cgccagtttc agcaccgcgc
     8761 gcgtgtcgcc gctaagcagt tccagttcca gctcacagat aggttcagca aattcccccg
     8821 ctttcacctc cccctgatcg agagcgattt caattcggct gccatcgacc gccaccagcc
     8881 atttttcgcg ataaaaatcg gtgctgaaca gcggttgtac gcgggaagcg agatcggcag
     8941 gcaattcacc gttcggccag acttccgagg gaaactgcgc caggtcgagc gtcggttcgc
     9001 tcaacgccac gttatattcc gggcgctggt gtaagccgcc tgtcactctt cctgcaactt
     9061 tcatggtcat ctcatagcga ccgttttcgc cacgaatacg taagcccata tcgtgcctac
     9121 gcagccagtt atccggcgtt tcgtagtaaa tattcagcaa ctgcacgggg tcatggtgct
     9181 cgccgcccag cgtattgaga tggtcacgca acgcctcaac ggcactgtga ttaacaataa
     9241 actttaattc gatttcctga gccatggcct ttacttatcg gttatgtcac ataagtgacg
     9301 atgaacggcg aacttaatgc gatctttttt gtcagtagat agtattttgc gccaaattgc
     9361 catgcaacgg gcaatttgac gggcgtaaaa gtttgaagca gtggcaagac ctgacacaga
     9421 ggattccgat tgatgtcgaa tgctttgcgt agcgacactg atgccactac tatcgttcca
     9481 ctttccatga caataacgac agcctgatgc caaaattacg cctgatcgga ttaactttac
     9541 tcgcacttag cgcgactgcc gtctcacacg ctgaagaaac gcgctatgtt tctgacgaac
     9601 tgaatacctg ggtccgtagc ggtccgggag atcattatcg cctcgtgggc acggttaacg
     9661 ccggcgagga agtgacctta ttacaaactg acgccaacac caattatgcc caggtgaaag
     9721 acagctctgg ccgtaccgcc tggatcccgt tgaaacaact tagcactgag ccaagcctgc
     9781 gctcccgtgt gccagatctg gaaaatcagg tcaaaaccct gaccgataaa ctcaccaata
     9841 tcgataacac ctggaatcag cgcaccgcag aaatgcagca aaaagtggcg cagagcgaca
     9901 gcgtgatcaa cgggttaaaa gaagaaaatc agaaactgaa aaacgagctg attgtcgcgc
     9961 agaaaaaggt cgatgccgcc agcgtacagc tggatgacaa acagcgcacc atcatcatgc
    10021 aatggtttat gtatggcggt ggcgtgctgg ggcttggctt gctgctcggt ctggtactgc
    10081 cgcacctgat cccaagccgc aaacgcaaag atcgctggat gaactaaatc gccttcttcg
    10141 ttgcactgtc ttattattag gaattgtgta tttgacgaag ggatgatggc gtgaagattt
    10201 atctggtcgg tggtgctatt cgggatgcat tgttagggct accggtcaaa gacagagatt
    10261 gggtggtagt cggcagtacg ccacaggaga tgctcgacgc gggctaccag caggtaggcc
    10321 gcgattttcc tgtgtttcta catccgcaaa cgcatgaaga gtatgcgctg gcacgtaccg
    10381 aacggaaatc cggttccggt tacaccgatt ttacttgcta tgccgcaccg gatgtcacgc
    10441 tggaagatga tcttaagcgt cgcgatctga ccattaatgc gctggcccag gacgataacg
    10501 gcgagattat cgacccgtac aacggtctgg gcgatctgca aaatcgtctg ttgcgccatg
    10561 tttcccccgc ttttggcgaa gatccgttac gcgtactgcg cgtggcgcgt tttgctgcgc
    10621 gttatgccca cctcggtttt cgtattgccg atgaaactct gacgctgatg cgcgagatga
    10681 cccatgcggg tgaactggaa cacctgacgc ctgaacgggt atggaaagag acggaaagcg
    10741 ccctcaccac ccgcaatcca caggtgttct tccaggtact gcgcgattgc ggcgcactgc
    10801 gcgttttatt cccggaaatt gacgcactgt ttggcgttcc ggcccctgcc aggtggcatc
    10861 cggaaatcga cacgggtatt cataccttaa tgacgctctc aatggcggcg atgctgagtc
    10921 cgcaggtcga tgtccgtttc gcaactttat gccacgatct cggtaaagga ctgacgccgc
    10981 cagaactttg gccgcgtcat catggtcacg gtccggcggg tgtgaaatta gtggaacaac
    11041 tctgccagcg tctgcgcgtg ccaaatgaaa ttcgcgattt agccagactg gtggctgagt
    11101 ttcacgatct catccacacc ttcccaatgc tgaacccaaa aactatcgtc aaattgtttg
    11161 attccatcga cgcctggcgt aaaccgcagc gtgtcgagca actggcgctg accagcgagg
    11221 ctgacgtacg cggcagaacc ggttttgaat cagcggacta cccgcaaggc cgttggttgc
    11281 gcgaagcctg ggaagtggcg cagtcagtgc cgacaaaagc ggtcgttgaa gcgggattta
    11341 aaggtgtgga gattcgcgag gagctgaccc gacggcggat tgcggcggta gccagttgga
    11401 aggaacaacg ttgcccaaag cctgactgag tataaaacta aacgttaaca cgaaccatcc
    11461 cctcgcccct tcggggagag ggttagggtg aggggaacag accagcaccg gtgcgaacaa
    11521 tcacccccac cccggccctc tccctacaga gcgagggaga aaaccaggcc tgctcccgaa
    11581 ccttttctca atcaaaagaa cacgacgtac accgcagccg ccacaataaa gcgataaatg
    11641 gcgaacggga taaacgaaat gcgcttaatc aattgcagga aggttttaat cgctatcagc
    11701 gccaccacaa aagcggtgat aaacccaacg gcaaacatcg agatatcgcc ggttgtcagg
    11761 aagccccagc ttttgtagag atcgagcgcc gttgcgccca tcatcatcgg taccgccagc
    11821 aggaacgaga actcggaagc agcgtaacgg ctcacgccca tcagcatccc acctgaaatg
    11881 gtggccccgg aacgggagaa acccggccac agcgccagac actggaaaca gccaatcata
    11941 aacgcctgac gataggtcat atcatcaaga cccggcgcac gcggctcttt cggcttcagg
    12001 cattcggcgg caatcagcaa caaaccgcca acgaccagtg catacatcac atttatcggg
    12061 ttaaacaatg acttaatcgt gtcgtggaac aacagcccca gtaccaccgc cggaatcatc
    12121 cccagcaaaa tgtggatcag cgttaaacga cctttgcttt ctccttcgtg ctgcaacggg
    12181 cggccaaagt ggatgccaat caggccaaac agacgccgcc agaacatcac tactaccgcc
    12241 agaattgatc ctaactggat cacaacttca aaggttttcg ccgtgtcgcc ctcgaacccc
    12301 aacaagtgac cgacaataat catatggccc gtgctggata ccggcagaaa ttctgtcaat
    12361 ccttcgacca cacccaatat tgccgctatc agcagcgagt gcatatcgct catcaataaa
    12421 cccctaaatt attaaaatgt accgcttgtc cggactactg cagtaagacc aggttataac
    12481 cgtttggttt aacagctgta aaattaatta ttttctttca gattattgcc acgctcaatg
    12541 attacaccaa cattcgccgc ccgcgccatt gcgcctggct tgctgagttt gatgcgcacc
    12601 cacggcgagt tgaagcgtgc taacagcagc tccgccacct cttcagccac gcgttccacc
    12661 agcgcaaaac gcgccccctc gacgtggctg accaccgttt ctgcaatgtc agcgtaactg
    12721 aggcaatccg ccacatcatc acttttcgcc gctttacggt tatcccatgc catttcgata
    12781 tcgaacacta acttctgttc gatggtctgt tcccagtcgt aaacaccaat agtggtgatt
    12841 accgaaagtt gctctataaa tacaatatcc atcacgtcct gcctgctttt tggctaaccg
    12901 gataccactt ccggcgaaat gtgcgtatta tccacagatt catcgttgaa cacgaatttt
    12961 caaaacggaa cagcttatga gtgcaatcgc gcctggaatg atcctcatcg cgtacctctg
    13021 cggctccatt tccagtgcca ttctggtttg ccgcttgtgt gggctgcccg atccgcgaac
    13081 cagcggctcc ggcaatccag gcgcaaccaa tgtgttacgt atcggtggca agggagcagc
    13141 cgtagcagta ctgattttcg acgttctgaa aggaatgttg cccgtctggg gcgcgtatga
    13201 attaggtgtc agccccttct ggttaggctt aattgccatc gccgcctgtc ttggacacat
    13261 ctggcccgtt ttcttcggat ttaaaggagg aaaaggcgtt gctaccgctt ttggtgccat
    13321 cgcacccatt ggctgggatc tcaccggagt aatggcggga acctggttac tgaccgtgct
    13381 attgagcgga tactcgtcgc tgggagcgat tgtcagtgca ctgattgctc cgttttatgt
    13441 ctggtggttt aagccacaat tcaccttccc ggtttcgatg ctctcttgcc tgatcctgct
    13501 gcgtcatcat gacaacatcc agcgtctgtg gcgtcgtcag gagacaaaaa tctggacgaa
    13561 attcaaaaga aagcgcgaaa aggatcccga gtgatttctg gtggatctac atgacctgat
    13621 agccttcatc gggcttgccc agccgttgct ggcaccatgc cgccagaaat tccacgcaga
    13681 cacgtaattt catgctgcga tagagcggct cccggtaaac agcccagata ttggcgcttt
    13741 gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc aacacatccc
    13801 actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact atctcgccgc
    13861 tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg ttccccaact
    13921 cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga cgacttaatt
    13981 cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg cataatattc
    14041 ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca ttaatacgaa
    14101 tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat tcaaaatgca
    14161 cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata tggctgcgcc
    14221 caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg atttgcgtga
    14281 cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg taacaacgtt
    14341 ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt gttgccagcg
    14401 tattttcaag gatttggatc cgcttggtga caaatgcagg cgtctgccca agcgtcgccg
    14461 ctgcggcgct aaaactcccg ctgtggacga tttctaccag tacctgcaag tctttggcta
    14521 aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag tgtatttcgc
    14581 aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg ttgattcacg
    14641 aattgttaat tacatattca cggtggcaaa aaatataaaa ccacattttt agtggtagtt
    14701 tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat gagcgaaagt
    14761 aataagcaac aggcagtgaa taagttgacg gagattgtcg ctaactttac cgccatgatt
    14821 tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga tgccgaaacg
    14881 tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa agcgattgac
    14941 ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa agtcggttcc
    15001 cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga agaggcaacc
    15061 gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa caccggcaaa
    15121 aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa tgacgatgcg
    15181 gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc gaaagtgtta
    15241 atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat ctccaccctc
    15301 gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc ggtggaaacc
    15361 gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca tcccaatcca
    15421 aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg gattggtcca
    15481 caagggctga ctggcaacag ttcagtgatg ggcgtacata tcgaatctgc cgcccgccat
    15541 ccgtcaacca tcggcgttgc tgtctctacc ggctgctggg cgcatcgtcg cggcacactg
    15601 ctggttcatg ccgatctcac ctttgaaaat ctgtctcaca cccggagcgc gttatgaaaa
    15661 agatcctgac aaccccgatc aaagctgaag atctgcaaga tattcgcgtc gacgatgtga
    15721 tctacctgac cggtacgctg gtgacctgcc gtgacgtttg tcaccgccgc ttgatcgaac
    15781 tgaaacgtcc gatcccttac gatctcaacg gcaaagcgat tttccacgct ggccccatcg
    15841 tgcgcaaaaa cggcgataaa tgggagatgg tttccgttgg cccgacaacc agtatgcgta
    15901 tggaaagttt tgaacgtgaa tttatcgagc agaccggcgt gaaactggtg gttggcaaag
    15961 gcggaatggg gccgctgacc gaagaaggct gccagaaatt caaggcgcta catgtgattt
    16021 tcccggcagg ctgcgcggtg ctggcggcaa cccaggtgga agagattgaa gaagtgcact
    16081 ggacagagct cggaatgccg gagtcgctgt gggtctgtcg ggtcaaagag ttcggcccgc
    16141 tgattgtctc tattgatacc cacggcaaca acctgatagc cgaaaacaaa aagctgttcg
    16201 ccgaacgccg cgatcccatc gtggaagaga tctgcgagca cgtccattac atcaaataac
    16261 cctcccggag aggctcgccc cctctccttt ttcgcaggca taacacgatg aaaccttcca
    16321 ctgaatggtg gcgatacctt gcgccgctgg cggtcatcgc cattattgct ctaattccgg
    16381 ttcccgcagg gctggagagt catacctggc tctactttgc cgtttttact ggcgtgatcg
    16441 ttggactgat cctcgaaccc gtgccgggtg ccgtggtggc gatggtgggt atatccatca
    16501 tcgccatact ctctccctgg ctgctgttca gcccggagca gctcgctcag ccaggcttta
    16561 aattcactgc aaaatccctc tcgtgggccg tttccggttt ttctaattcg gttatctggc
    16621 tgattttcgc cgcctttatg tttggcacag gctatgaaaa aaccgggctt ggacgccgca
    16681 ttgcgctgat tctggtgaaa aagatgggac atcgcacgct gtttctcggc tatgcggtga
    16741 tgttctccga gctgatcctc gcacctgtaa caccgtccaa ctcggcgcgt ggtgcgggga
    16801 ttatctatcc catcatccgt aacctgccac cgctctatca atcacaacca aacgacagca
    16861 gttcgcgcag cattggctcg tacatcatgt ggatggggat tgttgccgac tgcgtgacca
    16921 gcgccatttt cttgacggcg atggcaccaa acttgctgtt aattggactg atgaaaagcg
    16981 catctcacgc cacgctgagt tggggcgact ggttcctcgg gatgttgccg ctcagtattt
    17041 tgctggtgct gctggttccc tggctggctt atgtgctgta cccgccggta ctgaagtctg
    17101 gcgatcaggt gccgcgctgg gcagagacgg aactgcaggc aatgggcccg ctctgttcgc
    17161 gtgaaaaacg gatgctgggg ctgatagtag gcgcgctggt gctgtggatt ttcggcggtg
    17221 attatatcga tgccgcgatg gtcggttaca gcgtggtggc actgatgctg cttctgcgca
    17281 ttatcagttg ggacgacatt gtcagtaata aagcggcgtg gaacgttttc ttctggctgg
    17341 cctcgcttat caccctcgct accggactca acaacaccgg ttttattagc tggtttggca
    17401 aactgttagc aggcagctta agcggttatt cgccaacgat ggtgatggtg gcgttgattg
    17461 tggtgtttta tctgctgcgc tactttttcg ccagcgccac ggcgtatacc tccgccctcg
    17521 caccgatgat gattgccgct gcgctggcga tgccggaaat cccgctgccg gtgttctgcc
    17581 tgatggttgg cgcggcaatt ggtctgggga gcattcttac accatacgcc accggaccca
    17641 gtccgattta ctacggtagt ggttatctgc caacggcgga ttactggcga ctgggggcga
    17701 tttttgggct gatattcctc gtgttgctgg tgattaccgg cttactgtgg atgcccgtgg
    17761 tgttgcttta agtgaggacg gtgccggaaa ttgctctccg gcacccaata gttttacgca
    17821 gccggtaact ccgccagcgg ccagcgcgga cgcacgctaa cgccgagatc cgccgtcgcg
    17881 cctgctttaa accgcaccat tccggcatag gcgatcatcg cgccgttatc agtacaaaac
    17941 tccggacgcg cgtagaacac ttcgccgcgg cgttttttca tcatttcagc cagcttcgcc
    18001 cgtaacgtgc ggttagcact cacgccgccc gccatgacca gtcgcttaaa gcccgtctga
    18061 tccaacgctc gcttacactt aatcatcaac gtatcgacca ccgcatcttc aaaggcgcgg
    18121 gcgatatcag cacgcgtctg gtcgtcggtg ccgttgtcac gaatcgtgtt cgccgcaaag
    18181 gttttcagac cagaaaagct gaaatccagc cccggacggt cggtcatcgg acgcgggaaa
    18241 acaaagcgcc cggcagtacc ctgagccgcc attttcgaca gtaacggtcc gccaggataa
    18301 tccagcccca gcagcttcgc ggttttatca aacgcttcac cggcggcatc atcgatagac
    18361 tcgccgagca gctcgtactg accaatgcca gtcacgctga ttaactgcgt atgaccgccg
    18421 gacaccagca gcgcgacaaa cggaaattcc ggcgggttat cttccagcat cggcgctaac
    18481 agatgccctt ccatatggtg taccgggatt gccggaacgt tccaggcaaa cgccagagaa
    18541 cgccccacgg ttgcgccaac cagcagtgcg ccgactaatc cagggcctgc ggtataggcc
    18601 acagcatcaa tgtcttttgc cgttaagcca gactccttta gcgccgcctg gatcaacggt
    18661 acggttttgc gcacgtgatc gcgggaggcc agttcaggca cgacgccgcc atagtcagcg
    18721 tgcaatttca cctgactata caattggttg gctaacaaac ctttttcatc gtcgtaaatg
    18781 gcgatgccgg tttcatcgca ggaagtttca atacccagta cacgcatgac ttgttttacc
    18841 tcgctttatt accgcgcagt gtaggaccaa tgcgggttga tgtaaaactt tgttcgcccc
    18901 tggagaaagc ctcgtgtata ctcctcaccc ttataaaagt ccctttcaaa aaggccgcgg
    18961 tgctttacaa agcagcagca attgcagtaa aattccgcac cattttgaaa taagctggcg
    19021 ttgatgccag cggcaaaccg aattaatcaa aggtgagagg cacatgccgg taattaaagt
    19081 acgtgaaaac gagccgttcg acgtagctct gcgtcgcttc aagcgttcct gcgaaaaagc
    19141 aggtgttctg gcggaagttc gtcgtcgtga gttctatgaa aaaccgacta ccgaacgtaa
    19201 gcgcgctaaa gcttctgcag tgaaacgtca cgcgaagaaa ctggctcgcg aaaacgcacg
    19261 ccgcactcgt ctgtactaat tccccgagag cgttgctctc cgatcagacc gagttgtagt
    19321 tgtaaggccg tgcttccgaa aggaatgcgc ggcttatttt cgtttatgaa ttgctaaaaa
    19381 tcggggccta tggctggacg aatcccacgc gtattcatta atgatctgct ggcacgcact
    19441 gacatcgtcg atctgatcga tgcccgtgtg aagctgaaaa agcagggcaa gaatttccac
    19501 gcgtgttgtc cattccacaa cgagaaaacc ccgtcattca ccgttaacgg tgagaaacag
    19561 ttttaccact gctttggatg tggcgcgcac ggcaacgcga tcgacttcct gatgaactac
    19621 gacaagctcg agttcgttga aacggtcgaa gagctggcag caatgcacaa tcttgaagtg
    19681 ccatttgaag caggcagcgg ccccagccag atcgagcgcc atcagaggca aacgctttat
    19741 cagttgatgg acggtctgaa tacgttttac caacaatctt tacaacaacc tgttgccacg
    19801 tctgcgcgcc agtatctgga aaaacgcgga ttaagccacg aggttatcgc tcgctttgcg
    19861 attggttttg cgccccccgg ctgggacaac gtcctgaagc ggtttggcgg caatccagaa
    19921 aatcgccagt cattgattga tgcggggatg ttggtcacta acgatcaggg acgcagttac
    19981 gatcgtttcc gcgagcgggt gatgttcccc attcgcgata aacgcggtcg ggtgattggt
    20041 tttggcgggc gcgtgctggg caacgatacc cccaaatacc tgaactcgcc ggaaacagac
    20101 attttccata aaggccgcca gctttacggt ctttatgaag cgcagcagga taacgctgaa
    20161 cccaatcgtc tgcttgtggt cgaaggctat atggacgtgg tggcgctggc gcaatacggc
    20221 attaattacg ccgttgcgtc gttaggtacg tcaaccaccg ccgatcacat acaactgttg
    20281 ttccgcgcga ccaacaatgt catttgctgt tatgacggcg accgtgcagg ccgcgatgcc
    20341 gcatggcgag cgctggaaac ggcgctgcct tacatgacag acggccgtca gctacgcttt
    20401 atgtttttgc cggatggcga agaccctgac acgctggtac gaaaagaagg taaagaagcg
    20461 tttgaagcgc ggatggagca ggcgatgccg ctttccgcat ttctgtttaa cagcctgatg
    20521 ccgcaagttg atctgagtac ccctgacggg cgcgcacgtt tgagtacgct ggcactgcca
    20581 ttgatatcgc aagtgccggg cgaaacgctg cgaatatatc ttcgtcagga attaggcaac
    20641 aaattaggca tacttgatga cagccagctt gaacgattaa tgccaaaagc ggcagagagc
    20701 ggcgtttctc gccctgttcc gcagctaaaa cgcacgacca tgcgtatact tatagggttg
    20761 ctggtgcaaa atccagaatt agcgacgttg gtcccgccgc ttgagaatct ggatgaaaat
    20821 aagctccctg gacttggctt attcagagaa ctggtcaaca cttgtctctc ccagccaggt
    20881 ctgaccaccg ggcaactttt agagcactat cgtggtacaa ataatgctgc cacccttgaa
    20941 aaactgtcga tgtgggacga tatagcagat aagaatattg ctgagcaaac cttcaccgac
    21001 tcactcaacc atatgtttga ttcgctgctt gaactgcgcc aggaagagtt aatcgctcgt
    21061 gagcgcacgc atggtttaag caacgaagaa cgcctggagc tctggacatt aaaccaggag
    21121 ctggcgaaaa agtgatttaa cggcttaagt gccgaagagc gatcgggaag cccccgacag
    21181 ccgcactgag aggcagcggc aaatatataa gtacgccctc gtaattatcg ttggcggtaa
    21241 acaaccgttg gatttcagcg ttaacggctg aaggacatcg ggtcaatcgc ccaacaccaa
    21301 cctcatgaaa taagtgtgga taccgtctta tggagcaaaa cccgcagtca cagctgaaac
    21361 ttcttgtcac ccgtggtaag gagcaaggct atctgaccta tgccgaggtc aatgaccatc
    21421 tgccggaaga tatcgtcgat tcagatcaga tcgaagacat catccaaatg atcaacgaca
    21481 tgggcattca ggtgatggaa gaagcaccgg atgccgatga tctgatgctg gctgaaaaca
    21541 ccgcggacga agatgctgcc gaagccgccg cgcaggtgct ttccagcgtg gaatctgaaa
    21601 tcgggcgcac gactgacccg gtacgcatgt acatgcgtga aatgggcacc gttgaactgt
    21661 tgacccgcga aggcgaaatt gacatcgcta agcgtattga agacgggatc aaccaggttc
    21721 aatgctccgt tgctgaatat ccggaagcga tcacctatct gctggaacag tacgatcgtg
    21781 ttgaagcaga agaagcgcgt ctgtccgatc tgatcaccgg ctttgttgac ccgaacgcag
    21841 aagaagatct ggcacctacc gccactcacg tcggttctga gctttcccag gaagatctgg
    21901 acgatgacga agatgaagac gaagaagatg gcgatgacga cagcgccgat gatgacaaca
    21961 gcatcgaccc ggaactggct cgcgaaaaat ttgcggaact acgcgctcag tacgttgtaa
    22021 cgcgtgacac catcaaagcg aaaggtcgca gtcacgctgc cgctcaggaa gagatcctga
    22081 aactgtctga agtattcaaa cagttccgcc tggtgccgaa gcagtttgac tacctggtca
    22141 acagcatgcg cgtcatgatg gaccgcgttc gtacgcaaga acgtctgatc atgaagctct
    22201 gcgttgagca gtgcaaaatg ccgaagaaaa acttcattac cctgtttacc ggcaacgaaa
    22261 ccagcgatac ctggttcaac gcggcaattg cgatgaacaa gccgtggtcg gaaaaactgc
    22321 acgatgtctc tgaagaagtg catcgcgccc tgcagaaact gcagcagatt gaagaagaaa
    22381 ccggcctgac catcgagcag gttaaagata tcaaccgtcg tatgtccatc ggtgaagcga
    22441 aagcccgccg tgcgaagaaa gagatggttg aagcgaactt acgtctggtt atttctatcg
    22501 ccaagaaata caccaaccgt ggcttgcagt tccttgacct gattcaggaa ggcaacatcg
    22561 gtctgatgaa agcggttgat aaattcgaat accgccgtgg ttacaagttc tccacctacg
    22621 caacctggtg gatccgtcag gcgatcaccc gttctatcgc ggatcaggcg cgcaccatcc
    22681 gtattccggt gcatatgatt gagactatca acaaactcaa ccgtatttct cgccagatgc
    22741 tgcaagagat gggccgtgaa ccgacgccgg aagaactggc tgaacgtatg ctgatgccgg
    22801 aagacaagat ccgcaaagtg ctgaagatcg ccaaagagcc aatctccatg gaaacgccga
    22861 tcggtgatga tgaagattcg catctggggg atttcatcga ggataccacc cttgagctgc
    22921 cgctggattc tgcgaccacc gaaagcctgc gtgcggcaac gcacgacgtg ctggctggcc
    22981 tgaccgcgcg tgaagcgaaa gttctgcgta tgcgtttcgg tatcgatatg aacaccgacc
    23041 acacgctgga agaagtgggt aaacagttcg acgttacccg cgaacgtatc cgtcagatcg
    23101 aagcgaaggc gctgcgcaaa ctgcgtcacc cgagccgttc tgaagtgttg cgtagcttcc
    23161 tggacgatta atcggtaggc cggatcaggc gttacgccgc acccggcact agtccctctg
    23221 cacaaacgcc acctttt
//
LOCUS       DAESHI010000063        22968 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.063, whole genome shotgun sequence.
ACCESSION   DAESHI010000063 DAESHI010000000
VERSION     DAESHI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22968
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.063"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..158)
                     /gene="cas6e"
                     /locus_tag="KW011_002521"
     CDS             complement(<1..158)
                     /gene="cas6e"
                     /locus_tag="KW011_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HBH7660827.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREEL"
     gene            complement(140..886)
                     /gene="cas5e"
                     /locus_tag="KW011_002522"
     CDS             complement(140..886)
                     /gene="cas5e"
                     /locus_tag="KW011_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HBH7660828.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(897..1952)
                     /gene="cas7e"
                     /locus_tag="KW011_002523"
     CDS             complement(897..1952)
                     /gene="cas7e"
                     /locus_tag="KW011_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HBH7660829.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(1964..2500)
                     /gene="casB"
                     /locus_tag="KW011_002524"
     CDS             complement(1964..2500)
                     /gene="casB"
                     /locus_tag="KW011_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HBH7660830.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            complement(2497..4059)
                     /gene="casA"
                     /locus_tag="KW011_002525"
     CDS             complement(2497..4059)
                     /gene="casA"
                     /locus_tag="KW011_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HBH7660831.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDISAPRADL
                     QGAVWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFI
                     QDFEALTGDKVPVASLLPEIPGAQTTKFNKDHFIKRGVTEYLCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWINVMPQDEADLPLPKKFDD
                     LVFPWLGPTRISELAGAVVTDEQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVLLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLSKWNKEIWLFARQDFDERVFTNPYEPVDLERIMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(4157..6856)
                     /locus_tag="KW011_002526"
     CDS             complement(4157..6856)
                     /locus_tag="KW011_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBH7660832.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGI
                     NTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGL
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKE
                     DSSQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
     gene            complement(7051..7203)
                     /locus_tag="KW011_002527"
     CDS             complement(7051..7203)
                     /locus_tag="KW011_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBH7660833.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(7468..8202)
                     /gene="cysH"
                     /locus_tag="KW011_002528"
     CDS             complement(7468..8202)
                     /gene="cysH"
                     /locus_tag="KW011_002528"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HBH7660834.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(8277..9989)
                     /gene="cysI"
                     /locus_tag="KW011_002529"
     CDS             complement(8277..9989)
                     /gene="cysI"
                     /locus_tag="KW011_002529"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HBH7660835.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(9989..11788)
                     /gene="cysJ"
                     /locus_tag="KW011_002530"
     CDS             complement(9989..11788)
                     /gene="cysJ"
                     /locus_tag="KW011_002530"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HBH7660836.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            12107..12469
                     /gene="queD"
                     /locus_tag="KW011_002531"
     CDS             12107..12469
                     /gene="queD"
                     /locus_tag="KW011_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HBH7660837.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            12547..13818
                     /locus_tag="KW011_002532"
     CDS             12547..13818
                     /locus_tag="KW011_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBH7660838.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            13809..14069
                     /locus_tag="KW011_002533"
     CDS             13809..14069
                     /locus_tag="KW011_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBH7660839.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            14086..14661
                     /locus_tag="KW011_002534"
     CDS             14086..14661
                     /locus_tag="KW011_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HBH7660840.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(14809..15669)
                     /locus_tag="KW011_002535"
     CDS             complement(14809..15669)
                     /locus_tag="KW011_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBH7660841.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(15666..16445)
                     /locus_tag="KW011_002536"
     CDS             complement(15666..16445)
                     /locus_tag="KW011_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBH7660842.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(16423..17373)
                     /locus_tag="KW011_002537"
                     /pseudo
     CDS             complement(16423..17373)
                     /locus_tag="KW011_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(17360..18793)
                     /locus_tag="KW011_002538"
     CDS             complement(17360..18793)
                     /locus_tag="KW011_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBH7660843.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGINLAG"
     gene            complement(18863..19679)
                     /locus_tag="KW011_002539"
                     /pseudo
     CDS             complement(18863..19679)
                     /locus_tag="KW011_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            19997..21274
                     /locus_tag="KW011_002540"
     CDS             19997..21274
                     /locus_tag="KW011_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH7660844.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            21301..22791
                     /locus_tag="KW011_002541"
     CDS             21301..22791
                     /locus_tag="KW011_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005137807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBH7660845.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKTWAWSED
                     AAVMEKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTGVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNWGMRIRSRAGRMRE"
BASE COUNT         5476 a         6095 c         5666 g         5731 t
ORIGIN      
        1 tggagttctt cccgacgata aagaaattgc ctttctttgc cgccagggaa aagatcccac
       61 agccactgat gcatcacata ttcaccgcga tccaccagat gcagcaactg cgcaggcgaa
      121 agctggccgg tatgtaaggt tattcttgag aggtacatgg ttcctccttg ctgagccacg
      181 gcccctgatt gacggtgcgt tccccaaaca gccactgctg acgatttaaa ggaacatccc
      241 ggcggcgtaa tattttgctc gcgaccaggc cgtcgtgttt cccttcccac cagcattcat
      301 cctgaagttt cgggagtgag actttcagtt tatggaaatg atcctgatac tgttggtatg
      361 cattacataa ggcatcacac gcgttgcctt cgagcagtaa cggcgcaaga ggtaatgcca
      421 gcggatgact ttttcgcccc agataaagcg gaaaaactgg atggcgtaaa ccgtcctgca
      481 actgttcgag gctgtaaggc gcatcggctg ttgttgccac cgccaccatc caccaggcat
      541 cggtgtagta gtcgcgccgg gagataatcg cgctcaaaag ctcagggtcg ctcaactctt
      601 cacgacggct gaaataacgt gctttacgca cctcttttgg catctggacc gtgtgataat
      661 cccgtgccca gcgcgggtta cggctggcgc aaaccaccag tgaatagtgg cgattaaacg
      721 cgtttaagcg ttcggtatca tcacgccgaa tccctacccc ggcagccagt agccccagca
      781 atgctgagcg cgaaggaagt tcatgggtat gacgaacttc acctggagca tcgacacccc
      841 aggacgccat cggcccatga agctgaaaaa tcagatattg gctcataagc cgccccttac
      901 gcgcagataa agtccagcac gtccttcatg cttccctgtt tgttcatcac gtcaaaactt
      961 gcgcattcgg tcttctgttc gtagaccgta ttcatgtttt cgcgaagcgt tgtaatacgc
     1021 tgcaccgcca catctaactg acgtgtgcca ttaatgggtt catagaaagc cgccgccaga
     1081 gagcgtggtt gatcggtgcc tttttctgcc agcgcccagg aagcataggc acggctggca
     1141 aagctatttt gtttaccggt tggggaaact ttaagcgcgg cttccgtaaa agcgcgtatg
     1201 gtctgattag ctaacgcttc gtcaccgccg aggttttcta ccagcagatc tttatcaatg
     1261 cagatatagg tgtagaaaag tgctgaaccg aatccggttt ctccgagatg cccggcaccg
     1321 gcatcttcag aggcctggcg caaatcatca acggcggtga aaaaatcatc ttcgacaatc
     1381 gtttcactga caccaaacgc atgcgcgacc tggcaggcgg cttcaacatt aaactcaggt
     1441 ttattcgcca gcatacgacc aaacatagcg atatctaccg ctatacggtc tttacgtaac
     1501 aacgcgagat cttcctcttt tggcgcgcgc ttttcttctg ccagttgatg agcaagcgct
     1561 tttacggcgt caaattctgc cgggctgata tgaactaatt gttcagtttc ggcgttagtg
     1621 agcggatctt ttggtttttt gtcattttta gctttcccaa gataatcagc aatttttgcc
     1681 gcccattcga tggctttttt atcttcaatc cctttctcaa tcaggatagt tgccgcctca
     1741 cgcgcaatac gcccactgcg aataccaata tggcccgcca gtgcctgttc aaaaagtgca
     1801 gaagtgcgcc acgcacgttt cagactttgc gaggaaacgc gcagacgcgt tgctccaccc
     1861 agcaccacag tttttggcgc accggtgtca tcacggttaa ggttggcagc agggtaagcg
     1921 gttaacacat gaagctgaat aaatgttgtc atgagatagt cctttattga ttttgttcgt
     1981 tatcggcgtc gccagcctgg taatattcca gcgcccagcg gatacggata aattcggttg
     2041 gccgctgctg gcggcggtga tgattgagca ggtcgtcgct ctcctggcac cagcggaaaa
     2101 tgccatctgc cagggagtca agattgacgg aaccgtttaa taatctgacg gcgcggcgta
     2161 actggcgcag taattcatcc ggtgttttta ctgctgacag gcgagtaaaa cgcccctttg
     2221 acattaccgc cgccagttgc gcggcaaaag gctgccgttc atcaatggct ttaacattgg
     2281 cgctaactgc ggctatcagc gccagtgcgg tgatacgcca ctcaggttca tcctgccact
     2341 ttatttgtct gttcttcaga aacaggcgaa atccatccgt cagacaaaca tcattaaccg
     2401 tcgtactacg ccgcagactg gcacgttcgc cgcgtttctc ctgcaattcc tcgtgccatt
     2461 tgcgcagcgt ggctttgtgc tcctctttta caatactcat tcagcagcct cctgcttttt
     2521 ctccctggcg gctttagcac tttgcttctc cgccgatgtt gtaaaatatt tcttgcgcgc
     2581 ggtcatgatg cgttccaaat caacgggctc ataaggattg gtgaatacac gctcgtcaaa
     2641 atcctgacgc gcgaataacc aaatttcctt attccatttg ctgagtaatt catccgcatc
     2701 ctgaccttct tcaatttggc gcactaacct caggaagcga tgctgagttt tgttccagaa
     2761 gtcgatatcc acaaaactaa aatcacccct tgcacctttt ggatcggaga accatgcttc
     2821 tttcaatgca ctccgtaaca gacttagaat ccgtgaagcc gtttgcgcag ccagccgcag
     2881 tttcggtatc tggccttctt ttttattgag cagcagcggg aaatgatgtt cgtaccaaca
     2941 gcgcgctttc atgttgtcga aatcatagcc aaatccccac aggcccactt ttgcctgctt
     3001 gagactgctg gcattaaaga gtttcaccac cagcgcggga agttccgtat tgttttctga
     3061 cttacccgtt tcgataaggc ctaaccagtc gcgccagatt aaaccgcccg gttgtggttt
     3121 gaccgagtaa aactcgccgc cctctttaag tagtacacgg taaggcgtta agggatgctg
     3181 ccacatggca tagttagcgc cgtaattttt ggtcgtcatc aggctcagaa gcgcgtcgct
     3241 ctgctcaccg caaatatcgc agttgccgac tgtggtggta ttaaaatcaa tacgaatacg
     3301 gcgaggcatt ccccagtacg cctggagttt attgacctgc tcatcggtta ccaccgcacc
     3361 ggccagttcg ctgatgcgcg tcgggccaag ccaggggaaa accagatcgt caaatttttt
     3421 gggtagcggt aaatcggctt catcctgcgg catgacattg atccacagtt tgcgccacaa
     3481 gggagtttgt tgattaccct gatattcctg taattcaatc agagtggtca tcggcccacc
     3541 gccgcgtaaa ccggtgcggt agcctttgcc acctgacggc gcattcaact gtagggagaa
     3601 cagagctaac gcagagcaat gtgggcacag gtattccgtt acaccacgct tgataaagtg
     3661 gtctttatta aacttcgttg tttgagcgcc gggaatctca ggcagtagcg aagcgaccgg
     3721 aactttatct ccggtgagcg cctcgaaatc ctgtataaat gaaggggaat ctggcccaaa
     3781 ctggaaagca tgttctaatg acagcaatgc ttcccgtagc ttttcagctt ccagtccgtc
     3841 ttcccagata tcatcccaac gacgatgatc ttttggcgcg aagctggttt gtagtagccc
     3901 cagcaaaaac tgccataccg caccctggag atctgcccgc ggcgcagaaa tatcgacaac
     3961 attttcatcc gccagatcga ctggcgccag tttgcctgtc gttccgtctt taaaacgaac
     4021 gggcaaccac ggggttgtca gaagtgaaaa cgagttcata cgcatcctct cgtaaaaaag
     4081 accatcccag gtcggttgtc catctttcca tccttttcgg tgtatcaact attcagagag
     4141 tcctgtcgca gacatctcaa ccaaccttgc caatcaatcc ttccgtagcc gaatacccgc
     4201 aactttcgtc atcgttgact aaaataacgt ttgccatctc cggatcttgt cgctgttcaa
     4261 tgcaccacag cctgaacgcc tctccctcca gtaaagaaaa ctcatcccga tgttttttcc
     4321 accagcttcg gcgcactctg acaacgctca tttcccatgc gtgagcaccg gtggcataag
     4381 gcttcaccac accgtcaata caggtagcca gccacaggga aacagactcc tcagccagac
     4441 gtgtcgacag tttttctggg aggtaatcgt tgatattggc ggcatagccg ggcctgaagt
     4501 tcagcacaaa ctttttagcc attgcgcgat cacagtaata tttgcccact tgctcctgct
     4561 cgctgcgggc aaatccttcc ggcattacca cgtcctcacc gtagacggat tcaataagaa
     4621 gccgggctgc gtgtggcatc tgaatagcac cttgctcacg cagcacacgc tgcgtcagcc
     4681 agattcgccc atgatcggga tagacaaatg cactgttacg catggcactg ccaaaccatt
     4741 cgtcgccagg agagtcgtcc cagacgggag ccagaatcag caattcaggg ggggaacgct
     4801 cgtcctttcc gtcacgcttt aactgaccat taatatcgcg gatatgccgc tgtaatcgtc
     4861 ctgctcgctg aatcagcaaa tcaacagggg ccaggtcgga gatcatttcg tccaggtcac
     4921 aatcgacact ctgctctaat acctgagtac aaataaggac ttttccggca cgctgtgagc
     4981 tatcttcttt accaaagcgt gccagtgtct ccgtttcaat tcgctggcga tcgctaaaag
     5041 caaagcggct atgaaaaagt gaaaggctgg aagcaggaat gacgccgcgg gcaagcagct
     5101 gacgatgaac cttaatagcg tcatcgacag aattccggat ccaggcgatg catttcccct
     5161 gacttaccgc cgattcgata cgcgcaatac actcttgttc actatgaagc caacccacgc
     5221 tgacgctacg ctcaacgtct ttgcgcgtcg ctacccggtg tgagttcaca tcggatttcg
     5281 tgacatgcgt cagccagggg taatcatcct tttccaggaa cggagcttct tgcaggccct
     5341 ctgtaccccg cgcaaaggca gcgacgagtt tgtcgcgctg ctgttgagat aacgtggcag
     5401 aaagcaaaat aacgctgttt ccgccacgcg cctgccgctc gatcaaccct tcaagaatgc
     5461 acgacatgta agcgtcacag gcgtggatct catcagccag caggattttg ttactcagcc
     5521 ccagaagccg cagattatta tgtttgaacg gcatcaccgc catcatcgcc tgatccagcg
     5581 tgccgacgcc aatttcagcc agcaacgcct tcttgttact gtcggcaaac caggccgcac
     5641 atccctgact gaacgtttgt tcatccggtt cttctgaccc gactaaatca ccagaccaga
     5701 gtgattcatt aaagcggtcc attaatgtgc gggcactatg tgccagcacc aggctggggc
     5761 gggattctgg cgaatagaaa gcaagccagg ttttgaccag ccgatcgtac atggcattgg
     5821 ccgttgccat tgttggcaga ccaaaaaaca gcccctgagc cttccccgca gctatcaacc
     5881 tgtgcgccag gataagcgcc gcttctgttt tacctgcgcc agtcacatct tccagaataa
     5941 ataactgcgg tccttgctgg ctgatatcca gatccagcac cttttgctgt aacggtgtcg
     6001 gattctcaat aaaaggaaac agcgtattaa ttccgttgaa aggcgcgact tttgctttta
     6061 gaggaaagac ggttaaggcg ttttgagcct gaactaaagt tttctgccag taacctttaa
     6121 tatccattgg gtgtgcgacg cgtggaaaaa atcgcgttga cgaacccgtc cagtctgcga
     6181 gcacgacggt tgcagagata taccaggaaa ggtgttttat aagttcaatg ccctcatcat
     6241 catcccagaa tgcggggatc tctatgagcg gaaacagtgg tttgatttca aggagaaaat
     6301 ctcgtgctgc agctttgtct tcaggcagaa aattatccaa ttcatccata cgatcagggg
     6361 gccgaccatg atgccctgtc gtcacgggca tccacatttc tatcactcgc ctaagtttgc
     6421 gcggagagag cgaagatgaa ggaagtaact cctgacattc acttaaataa tgattccaca
     6481 accagtagcc cagcgttgaa tgagagattt tttcgtaatt ttttctggca ccttcctgaa
     6541 tcttgagttc agggggcagg taaagttgtt gaaaagagcg ggcaaatttt ccaatatcgt
     6601 gccagcacag caaccaggcg aaaaattgag ccgcctgttc cttgtcagaa atccctaatt
     6661 gacgaaaata atcagccagc ccgaagcaat ttcttttaac cattaaatag cccatcgcgg
     6721 ctacatccag cgaatgccag caaagaaggt gatagtcgtc gcccccgtct ttctcgccgc
     6781 gtcgggtttt tccccagaaa tcaaaaaaag tcacaatatt tttatccttc agtaaactta
     6841 aaggatattt acgcatatat taaaaattat ctgtgatata tatcattcaa taaaatattt
     6901 acattatgaa taatcaaata attttcatcc gtaatatata tgaataataa acataggcat
     6961 aatattcaca taaataatat aaattaaaaa agggtgcagg agaccaaacc atcacaattc
     7021 agtacgcggg gttttacccc cgcgtggtgg ctacttcttc gattcgtaag cgagaactgc
     7081 cttaaactcc ataccacgtt ctctgatact caactcacac aacgagtcgc ctaccatcag
     7141 cgtgaatccc agtactgtaa aacacagcac tatgattgcc acaagggcat attttgtcag
     7201 catgtcttgc ctccctgcaa agaagagact aacattcgaa ttgtttgggt tcgaacgctg
     7261 gcctcaggtt gatagaaata tcgcctgggg cttttgtcca tctggaacct cgcgaatgct
     7321 taacgccaga cagcctcaag cacccgacgc cattctatac ctgataattc ttccagcggc
     7381 gttcacgttt aaaatcagga atactgctcg caaaataaaa acgcccccat catttctgac
     7441 agaggcgttt aatttatccg gcaatattta cccttcgtgc aatccacatt ccctttttaa
     7501 gccaaagaaa cgtgtttctt cttccgacat gccaggttcc catttacggg ttgtatgggt
     7561 atcaccgacc gataaatatc cttcatccca taatgggtga tatttcaggc catgtttttg
     7621 caggtactga taaatagttc ggttatccca gtcgataatc ggcagcactt taaatacgcc
     7681 acgctgaatt gccagcaccg gtaaattggc gcgactgccg gattgttcac ggcgcaggcc
     7741 agcaaaccag gtttgcgcgt taagttcttt cagagcccgg ttcatcggtt cgactttgtt
     7801 gatgtcattg tacttttcaa tgccttcaac gccctgctcc cacagttttc cgtagcgtgc
     7861 ttcctgccag gctgcgcttt cggtagcacg gtacactttc aggttgagct tgagtttgtc
     7921 cgttaactcg tcaataaagc ggtaggtttc cgggaacaag taacccgtat cggtgaggat
     7981 caccggaata tccgggcgaa tttgattcac cagatgcagg ctcaccgccg cctgaatgcc
     8041 aaagctggaa gaaagtacat attcaccggg cagattatcc agcgcccagg ctacgcggcc
     8101 ttcagcgtcc agtttttcca gttcggcgtt agtttccgcc agcgccagaa tacgatctac
     8161 cttcggcagt tcgttcaggg cgtttagatc gagtttggac atagattcct cactgtttgc
     8221 cttgcctgat gcgacgctca cgcgttctta tcaggcctac aagaccgggc tgatggttaa
     8281 tcccacaaat cgcgcgccgg atcgagcacc gggcgaatga tgcccgcacg caccgtaaag
     8341 tcgccgaagc cttcacccgc ttcgcgctct ttcgcccagc gccctatcag ttcatcaagc
     8401 gacgccagga tttccggctc ggtgatgttt tctttataca tccgtgggat acgtgtccca
     8461 atgcggttgc cgccaagatg caggttgtag cgacccggcg ctttacccac caggcccact
     8521 tccgccagca tcgcgcgacc acaaccgttc gggcagcctg ttacacgcat cacgatatgc
     8581 tcatcgctga caccatgttt cgccattaaa ttatcgatgt tgtcgataaa agacggcagg
     8641 aaacgctctg cttccgccat cgccagcggg caagtcggga atgacacgca ggccatcgag
     8701 ttttcacgct gcggcgtgac ggcattcatt aacccgctct ctttggcgat cttctcgatc
     8761 ttcgctttct cgctttccgg tacaccggtg atgatcagat tctggttcgc cgtaatgcgg
     8821 aaatcgcctt tgtggatctt cgcgatctcc agcaggccgg ttttcagcgg acgccccgga
     8881 taatcaagga tgcgaccatt ttcgataaac agcgtcaggt gccagttatc atcaatgccc
     8941 ttaacccagc caatacgatc gcctcgtccg gtgaactcat atggacggat cggttcaaat
     9001 ttgatccccg cgcgacgctc cacttccgct ttaaacgtct caacccccac gcgctccagc
     9061 gtgtatttgg ttttggcatt tttacgatcg gttcggttac cccagtcacg ctgagttgtc
     9121 acgacggctt cggccaccgc cagcgtatgc tccagcggca gatagccaaa ctcgctcgcc
     9181 gtgcgggcgt aggttttctt gttgccgtgt tcgatagaaa gcccaccgcc caccagcagg
     9241 ttgaagccta ccagcttgcc gttttcggcg attgccacga agttcatgtc gttggcatgc
     9301 agatcgatat cgttctgcgg cgggatcact accgtggttt tgaacttacg cggcaggtag
     9361 gtctgaccaa ggatcggttc ttcatcagta gtggcgactt tttcctggtc gagccagatc
     9421 tccgcatacg cgcgggtacg aggcaacaga tgctcagaaa tcttcttcgc ccactcgtac
     9481 gcttccgcgt gcagctgcga ctcgtaaggg ttcgaggtgc agagtacgtt acggttcatg
     9541 tcgttagctg tcgccagcgc atcaagaccg accgagtgca gcatctggtg caccggtttg
     9601 acgttctttt tcagaatgcc gtggaactga aacgtctggc ggttggtcag gcgaatgctg
     9661 ccatagatgg tgttttcacc ggcaaatttg tcgatcgcct gccactgttt agtggtaatc
     9721 accccacccg gcagacgaca gcgaagcagc atcgcgtggc gcggctccag cttctgttca
     9781 gcacgttcgg cacggatgtc gcggtcatcc tgctgataca tgccgtggaa gcggatcagc
     9841 aggaagttgt cgcccttaaa gccgccggtc agaccgtcgt ttaaatcttc cgcaatggtg
     9901 ccgcgcaggt agttgctttc aagcttcatg cgctcggcgt ctgtcagttt tccttcgacc
     9961 actaaaggcc ctggatgttt ttcgctcatt agtagacatc tcgctgataa cggcgctcta
    10021 cgcgcagctc acttaaaaat tcatccgccg cttcggtgtc catgccacca aattcggcaa
    10081 tcacttccag aagtgcctgc tcaacgtctt tcgccatgcg attagcgtcg ccgcagacat
    10141 aaatgtgggc accgtcattg atccagcgcc acagctccgc gccctgttcg cgcagtttgt
    10201 cttgtacata aactttttct ttttggtcgc gcgaccaggc aagatcgata cgcgtcagca
    10261 cgccctcttt gacgtagcgc tgccactcca cctggtagag gaaatcttcc gtaaagtgcg
    10321 ggttgccaaa gaacagccag tttttacccg gcgcttcgtc ggcggcgcgt tgctgcataa
    10381 aggcacggaa cggcgcgatg ccggtaccgg ggccaatcat aatcactggc gtttgcgggt
    10441 tagcgggcag gcggaaattg tcgttatgtt cgataaatac gcggacttcg ccctcttctt
    10501 ccacgcgatc cgcgaggaag ctggaggcac caccggcacg ggcgcggcct tccacgtcgt
    10561 aacgcaccac accaacggtg acgtgtactt cgttctcgac ttccgcctgc gaggaggcga
    10621 tggaatacag gcgcggggtc agcgggcgca gcaggttaat cagcgcttcg gcatccagtt
    10681 gcgccggaga gaaacgcacc atgtcgacaa tcggcgtggt cgcggcgtaa tgctgtaact
    10741 tcgctttatc gcccaccagc ggcagcagcg tttcgctgcg ggtaagcgtg gcatagttct
    10801 caacaatgtt ggcggtgttg acggtcagtt cgaagtgcca ctgtagcgct tcgttcagag
    10861 gcaacgtttt gccctcgacg gtgacaggtt catcgccttt cagccacagc agttcgacaa
    10921 gttctttcac cagtgccggg tcgttctgat accagacacc cagcgcgtca cccggctggt
    10981 aacgcaggcc tgaatcgcct aagtcaattt cgatatgacg aacgtctttt tcagagttac
    11041 gcccggtaat tttctggtta accgaaaggc tcgccaccag cggcgcgtct ttgctgtacg
    11101 ggctggtgtg gatttcattt accgcgccag tagcgacgga ttgcgaaggt gccgcgacag
    11161 gcgcacgcga tttaagcgca tcaaccacgc gggcgcgcca ctcgctggca gcagcctggt
    11221 attcaacatc ggcatcgaca cggtcgagca ggcgttcacc acccagttcc gccagcttgc
    11281 tgtcgaaatc tttcccggac tggcagaaaa attcataaga gctatcgccg aggctaaaca
    11341 cggcaaacgc ggtgttttcc agctttggcg cttttttgga gaacaggaac ttatgcagcg
    11401 cgacggcttc ttccggcggt tccccttccc cttgcgttga cgtcactacg atgagcagtt
    11461 tttcgctggc gatttgtttg aatttatagt cgcccgcgtt caccagctta acgttcagtt
    11521 ttgctgctaa taaatcatcg cgtaatgctt cagcaacccg gcgcgcattg ccggtttgcg
    11581 aggcggagat aatagttata cccggcattt ctgcggctgg cgctggcgtc gctgcaagcg
    11641 cagcaggctg ctgattgagt acgccccaga aatagccaga aacccaggca agctgggtag
    11701 gcgttaaatc ggtcgtggcc gcctgaaggc gtgccagttg ctccgggttc aacggaagca
    11761 acgcggaagg tgggacctgt gtcgtcatgc gtcgttatgt tccagtaagc aaagctgttt
    11821 ctgcgccctg tcagcgccca taaaacagaa gagaagataa ggttaacggg gtgaacggtg
    11881 tggattaaag acgggatagc gataactaat aaccaaatcg actaacctgt tttagcaata
    11941 gtctttaaca acaaaataga ttaaccaacc caatgaaaaa caaatgaatt tagccaatca
    12001 ttaagataaa tcagcgattt tgcgcaacaa agtcgtttta gataatgcga aaaaacagcc
    12061 tttccggtac tctacggcgg ttttatcgtc ctgtagagaa attatgatgt ccaccacgtt
    12121 atttaaagat ttcaccttcg aagccgctca ccgcttacca cacgtcccgg aagggcataa
    12181 atgtggtcgc ctgcacgggc attcctttat ggtgcgactg gaaattaccg gggaagtcga
    12241 tccgcatacg ggctggatta tcgatttcgc tgaactgaaa gcggcgttta agccaactta
    12301 tgagcgcctc gatcaccatt atctcaatga tattccaggt ctggaaaacc caaccagcga
    12361 agttttagca aaatggattt gggatcaggt taaacccgtt gtgccgctgt taagtgcggt
    12421 gatggtaaaa gaaacctgca ccgcagggtg tatctatcgc ggcgaatgat aagagtgtgt
    12481 cggcggtcaa tttcccttaa gtaacgctat gttagggtgt tgtgttctgg atatctgggg
    12541 catgacatgg aagacgactg cgacattatt attattggtg ccggtattgc aggcaccgct
    12601 tgcgcgttac gctgcgcgcg agcgggttta tccgttttgt tactggaacg cgctgaaatc
    12661 cccggcagca aaaatctttc cggcgggcgg ttatataccc atgcactcgc ggaactcctc
    12721 ccgcaatttc atctgaccgc gcctcttgaa cgacgcatca ctcacgaaag cctttccctg
    12781 ttaacgcccg atggcgtaac gacgttttcc agcttacagc ccggcggtga atcctggagt
    12841 gtattacgtg cacggttcga tccgtggctg gttgccgaag ccgaaaaaga aggtgtcgaa
    12901 tgcatccccg gagcgacggt ggatgcactg tatgaagaaa acggcagagt ctgtggcgtt
    12961 atttgtggtg acgatattct ccgcgcccgt tatgtggtgc tggcagaagg tgccaacagc
    13021 gtcctggctg aacgtcacgg gttagtgact cgtcctgctg gcgaagcgat ggcgttgggg
    13081 atcaaagaag tgctgtcgct ggaaacatcc gctattgaag aacgttttca tctggagaat
    13141 aacgaaggcg cagcgttgct gttcagcggc ggaatctgtg atgacttacc cggcggcgca
    13201 tttctttata ctaatcaaca aacgctctcg ttagggattg tttgcccgct ctcttccctt
    13261 acgcaaagtc gtgttccggc aagcgagctg ctgactcgct ttaaagcgca tccggcagtg
    13321 cgcccgctta tcaaaaacac ggaatcactg gagtatggtg cgcatctgga gccagaaggt
    13381 ggcttgcaca gtatgccggt gcaatacgcc ggtaacggct ggctgctggt gggcgatgcg
    13441 ttgcgcagtt gcgtcaatac cggaatttcc gtgcgcggca tggatatggc gctgactggc
    13501 gcacaggcgg cggcacaaac gctaataagc gcctgccagc accgcgagcc gcaaaatctg
    13561 tttgcgcttt atcatcacaa cgtagagcgc agcctgctat gggatgttct acaacgttat
    13621 cagcatgttc cggcgctttt gcaacgccct ggctggtatc gggcgtggcc tgcgttaatg
    13681 caggatattt cccgcgattt atgggatcag ggtgataaac ctgttccacc gctgcgccag
    13741 ttattctggc gtcatttacg tcgtcatggc ctgtggcatc tggcgggcga tgttatcagg
    13801 agtctgcgat gtctgtagcc cataatctct ggcgcgttgc tgatgcgccg cacattgttc
    13861 cggctgactc cgttgagcgt cagatggtac aacggttgat taacgcctgt ccggcaggtc
    13921 ttttttcgct cacaccggaa ggtgacttac gcgttgatta tcacggctgc ctggagtgtg
    13981 gcacctgccg tttgctgtgc gacgaatcca cactacaaca gtggcgctat ccgccttccg
    14041 gattcggcat cacctaccgc tttggataaa taataaggat aatttatgcc cctcttacac
    14101 ctactccgcc agaatccggt gattgctgcc gttaaagata atgccagcct gcaactggct
    14161 atcgattctg aatgccagtt tatttccgtg ttgtacggca atatctgcac catcagtaat
    14221 atcgttaaaa agattaagaa cgccgggaaa tatgctttta ttcatgttga tttgctggaa
    14281 ggcgcgtcaa ataaagaagt cgttattcag tttttgaaac tggtgaccga ggcggacggc
    14341 attatcagta ccaaagcctc aatgttgaaa gcggcaagag cggaaggttt tttctgtatt
    14401 catcgcctgt ttattgttga ttcaatttcg tttcacaaca ttgataagca agttgcgcaa
    14461 tcgaatccgg attgtattga gatcctgcca ggctgtatgc ccaaagtgct gggctgggtg
    14521 acagagaaaa tccgccaacc gctgattgcc ggtgggctgg tgtgcgatga agaagatgcg
    14581 cgtaatgcga ttaacgcggg tgtcgtggcg ctttccacca cgaataccgg ggtctggacg
    14641 ttagcgaaaa aattactttg acgggataat cgtaaccaat tgaatttggt ttgatttgta
    14701 ggccgcacgc cacatccgac attcagcgcc tgatgcgacg cttgacgcgt cttatcaggc
    14761 ctacaagtcc ccaggccaga accgtaggca ggatcatccg gcaacacgtt attggcaatc
    14821 tgcgtgaatg tttgtcacca gtgcctccag caccactttc cagtcatcaa ccacgccaac
    14881 atctgcctgt gaaaataccg cagcgctggc gtcatgatta atcgccacca caaacttgct
    14941 attgcgtacg cccgccatta acgctgctgc gccagaagcg ccaacaacaa tacacacctc
    15001 aggtgccagc agatgtccgg aaataccaat cactttttca gcatcaacgc caccgttcat
    15061 cactcgcgcc cgactgtagc ccacttcggc tcccagcttt tctgccagca tagcgatttc
    15121 ctggttatct gcctctccgc cctgccccac caccagcacc cgtctggcct cagctaatgg
    15181 gtcgcgagta acatttttca gatcctctgt gctaaccagc cagtccggaa gcgcacccgg
    15241 gacaatgttc agttgttgca ttccagaggg caacgtggcg tttttcgctg ctcccgcctg
    15301 acgcgccagg gaaaggcata gcgggcgttt ttctgtttgc agcgtcgccg ttagcgcatt
    15361 tccccagtgg gatttgcgca cgcttacggt tggaatatct aacgaagtca cctggcagat
    15421 gcttgcacca tgtaaacgcc aggccagccg ggtcgacagt tcatcgccaa acgttcccgg
    15481 cggaaagaga acaacgtccg caggcgttcg ctgccactgt tccaccaacg catccagcac
    15541 ctgttccgcc actacaggct gaggttcaat ctgccagtgc gccagggtac aaccgctgaa
    15601 atcctgcgcc gccagccagc ttgctattgc ggcattctcc tgatttattg tcacaattgc
    15661 gatattcatg gctgcatcct ttggcgtaaa tagtcctgcc atagcttttg agctttttct
    15721 gctaccgttt gcccatcaat cagcgtcgct ccgcgtcgtt gctctgctct tgccagttgc
    15781 agacactgca tcgctggcat ttcggcagca acagttttac ggataatttc tgctttcccc
    15841 gcagccattc gctggcgcat tcccggtact ggcaaggcca cttcaccgca ctgacgcacg
    15901 gcaatcaccg caggcaagcg aacccggcaa caacgcaacc catgttcagt acgctgttcg
    15961 agggtgataa acagcgcgtc gagagtgaaa cgctccacct gggtgaagca gggccagccc
    16021 agcatttccg ccagcaaaaa tggcgtttgc ccattctgcc cttcgctgct ttggcagccg
    16081 gtaattatca gatccagcgg attctgatgc tgccattcgg caatatggcg agcgacaaat
    16141 tccggcgcaa agcgcagatc tgccgccgtc tccagcaata cagcttcctc aaacccaaga
    16201 gccatgagat agcgcagcca gtgcaatgcc cgttcatccc ccatgctcaa cgcggttaaa
    16261 gacatcggcg taccgttttt tctctgcgcc aacagcagcg cggcagcggc ctgttcatca
    16321 gcaccgagta aacttcgcag tagcgaaata tccggtccgc ttttaccctg agccgccgcc
    16381 tgccactctt tttccgccag cattccggca tccggttcgg ctttaaacgc taacagaatg
    16441 ttcattcgca cctcctacat ttcccgccgc caccagcggg agggctttag tttctggtgc
    16501 ccatagccag gtcacaacaa aatcaaccaa caacactgtc gccaggagca ataaggtgac
    16561 ttgcattccc cactgcgcca gcacccacgg cagcaggcca gtacttaccg ccgcgccaag
    16621 tcgactcatg gcggtggcaa aaccgacgcc cagcgagcga atgtcagtag gaaaactttc
    16681 cgcaggcaaa atgcccacca gattactgac tgccgaaatg gtggtgctga agagaacaaa
    16741 aagcagcagc gttaatgaac tgccggaagg caaacaggcc atcactacca gcgttgccgc
    16801 cagcagcaaa aaacttccca gcaaaaattt gcgatgtgcc agcaggtgcg tcagaactaa
    16861 tcccagcagc gcgcccacaa ttaacaacgc attaagcatc aggctggcag tcagcgcatc
    16921 ttccagacca atagtctggg cgatagttgg cagccaggta taaatcacaa accatgggat
    16981 tacgaggcag acaaagaaga cgctgttaaa cgccgtgcgc cgccagtaac gcgaagagaa
    17041 caaggttttg atgtgtttat gggtcgccgt taccacttca tcgcccagta aaacatgggg
    17101 accaaaatag cgatgcacga tagcgtgagc ttctgcaaaa cgcccctggc gcagtagcca
    17161 gcgtggtgat tctggcgttc cccagcgtaa taacgtaatc aacaacgcgg gcagagctgc
    17221 cgatgccagt agccagcgcc aggcctccgg gttttcggaa ataaagtgat gtccggcaat
    17281 acttgccagc acatagccta cggtccacac cacgctgaat gcgcccagca aaataccgcg
    17341 atggcggcgc ggggaaaatt cagccagcaa ggttaatgcc attaggatcg aactgctttt
    17401 tcagcccttc cagcaacgcc cacgcgctgc cgtgttccag tttgctccag tgaacgcgat
    17461 gtttaccgat accgtggtgg tgcaccatcg aaccgccgag gcgaatggtt tcttcacaga
    17521 tgatcttgtt gagcggattg tggtacttgt cgatttcctc ttccggctta cagtcaacga
    17581 cgttgtaatc gtagacgaag tacatgttgg tgccgttctg atagctatga gaggaatgac
    17641 cgcccagcat ggtgatgtcg tcggcgtgcg ggaactcagt acgaatacgg ttaataacgc
    17701 tttcgtagat ttcgtggatg cagctccagc agccggacac ttcagtggta aagcccatgt
    17761 tgccggtttt gaggatctgc acacgttcgg cagccacttt atccggtccc cagttcaggt
    17821 tgttaaacca ggtttcgatc agcttgctgt ccacgcgctg gcattgcggg tagcgggcaa
    17881 cgatttccgc aatcccttcg cccgtcgcct tcgcaatgcg agggttacct tcagccataa
    17941 agatcagcac gcattttccg tctgcaaaat gggtgaagtg ttgggtgcca tcttcagcgt
    18001 catacaaacg agcgatcgac ggacgatacc cttccaccat gacttcacgc aggatgttga
    18061 agtcggtttt catgtcttcc aggatatagc cgtagaagag gttgttttcc ggggtgaatt
    18121 taaagatttt cactgttact tcagtgatat agcaaagtgc accttcgtta ccgatgatga
    18181 tgtggcgaat gtccgggcca gccgcgcggc gtggcacgtt tttaatgcgt gtgacggtgc
    18241 catcagccaa tactgcttcc agaccaacga ccatatcttc gattgcgccg tagagtgtgg
    18301 agaactgccc gatactgcgg gttgctacca agccgcccat ctgcgccagc ggctttgact
    18361 gcggagaatg ccccgtggtg taaccttttt cacgcaacgc attttccagc acctccagcg
    18421 gaacgccaca ttgcgccgtc gcctgcatat tctcaatatc aatattaatg atttgattca
    18481 tggcggagcc gtcaagcacc accgagtttt ccacaacggt ttccagccca ccttcagtgg
    18541 cggaagcgcc ggtacgcggc acaccgttaa ttttgtgcgc attcataaaa ttcagcacac
    18601 gggatacttg ctctgtggaa ccgagtttta cgaccgctgc cggaatcggc aaagtataaa
    18661 tgccatgaat atccggaaat ttacgaaaac ggtcaatact gttcttcttt aatactgttt
    18721 catcggtaat tacgcgatct gcaccaacaa tttcctttag ttggtcgaca atcgctgcgc
    18781 gagataaaga catagtaatt ccttcctgat aataaaaatg gaataatcat taataagaga
    18841 aaacagaaac caccgttatg gattagcgca ctaaataacc accattgacc actaataaat
    18901 gcccattgac ataatttgaa gccggacttg cgaggaatac agctgcgccc attaaatcct
    18961 gagtatcgcc ccaacggttt gccggaatat gatcaagaac gcgctgattg gtttctggat
    19021 tactgcgtgt cgccagcgta atatctgttg cataatagcc aggagcaata ccatttacct
    19081 gaatattata ttgacctagt tcatcacaat aagctttggt gaatccggca agagcatgtt
    19141 tagtggcaga atatgcaggt gaccattgtc cacctaagta agagaacaat gaacagatat
    19201 taataatttt gccgcttttc tgcgggatca taatttttgc agcttcatag cttaactcga
    19261 atgcggcggt caggttcaca tcaatcatcg gatcccagtc ggcacgaccg aagtccagca
    19321 ccttattcag cttacaaata ccggcattgt ttaccagaat atcaactgta ccgaaacgct
    19381 cacagcaagc agcgataatc ttctgcggcg cgccttctgc ggtgatatcc acctgcatga
    19441 agtccacctc aacaccctgt ttttcaaaca tttcctttgt ttcgccgtta tctttcaatc
    19501 atttcctttg tttcgccgtt atctttgacg aaactaggaa taaagatatt tgcgccagct
    19561 ttggccaacg ccatggcaaa tgcctggcct aaaccgctat tcccaccggt aacaattgcg
    19621 gttttacctt tcagtgagaa aaaatccatt gagaacgcat tgagagattc gattgacata
    19681 gttagctcca atttccgtaa ggcaaaaaaa agaaaggtaa cttcccccgc gagagggaag
    19741 ttacctttct cattatctct ggtaactgga gataattttt agcataacgg cataactgct
    19801 aatgtgatcg taatcacagt gtgatattcg tattgtcaaa tagttttaaa aagcagaagc
    19861 gtgattactc tcacacaaca cctctgcttt ccatgttatt tcttattgca attactagag
    19921 acctgagaaa agtaatccac ctgaaagggc ggcttacttt tctctttttt ttgtctatta
    19981 caaatgggaa tacgatatgc aacacaactc atatcgccgt tggataaccc tcgcgataat
    20041 tagttttagc ggcggcgtta gttttgacct ggcttattta cgttatattt atcaaattcc
    20101 catggcgaaa tttatgggat tcagcaatac cgagataggt ttaataatga gtacctttgg
    20161 tattgcggct attattcttt atgcccccag cggcgttatt gccgataaat tttcacaccg
    20221 caaaatgatt acttccgcga tgatcattac cggattactg ggtctgttaa tggcaacgta
    20281 tccaccgctg tgggtaatgc tctgtattca ggtcgccttt gcgataacga cgattttaat
    20341 gctgtggtcg gtgtcgatta aagccgcctc gttgctgggc gatcatagtg agcaagggaa
    20401 aattatgggc tggatggaag ggctgcgcgg cgtcggtgta atgtcgctgg cggtgtttac
    20461 catgtgggtc ttttctcgct ttgcaccgga tgacagcgcc agcctgaaaa cagtcattat
    20521 catctacagt gtggtttaca tcttgttggg gattctgtgc tggttttttg ttagcgataa
    20581 caacaacctg cgcagtgcca ataacgaaga aaaacagtca ttccagctta gcgacatcct
    20641 tgccgttttg cgtatcagca ccacctggta ttgcagcatg gtgatttttg gtgtcttcac
    20701 aatctacgcc attctgagtt actccaccaa ctatctgacc gaaatgtacg gcatgtcgct
    20761 ggtggcagcg agctacatgg ggattgtgat caacaaaatc ttccgcgcgc tgtgcggccc
    20821 acttggaggc attatcacca cctacagtaa agtgaaatcc cctacccgcg tgattcaaat
    20881 cctttccatc atcggtttgc tggcgttgac agccctgctc gtcacgaact ctaaccccca
    20941 atcggtcgcg atggggattg gcctgatttt actgctggga ttcacctgtt acgcctcacg
    21001 cgggctgtac tgggcctgcc ccggcgaagc gagaacaccg tcttacatta tgggcaccac
    21061 ggtaggtatt tgttcggtga ttggattcct gccggatgtc ttcgtttacc cgattatcgg
    21121 ccactggcaa gacaccctgc ccgctgcaga agcctaccgc aatatgtggc tgatgggcat
    21181 ggcggcgctc ggcatggtga ttgtctttac ctttttgctg ttccaaaaaa ttcgtactgc
    21241 tgatagcgcc cccgcaatgg ctagcagcaa gtaagccagg aaaattacag gagaaaaatc
    21301 atgtcgaaaa aatacatcat agggattgat ggcggaagtc agagcacaaa agtggtgatg
    21361 tacgatctgg aaggtaatgt ggtttgcgaa ggtaaaggct tattacagcc gatgcacacg
    21421 cctgatgccg atactgcaga acatcctgac gacgatttat gggcatcatt atgttttgca
    21481 ggtcacgatt tgatgagcca gtttgccggg aataaagaag atattgttgg catcggcctg
    21541 ggctccatcc gttgctgccg tgcgttattg aaagccgacg gcacgccagc tgcgccgttg
    21601 attagctggc aggatgcacg cgttacacgc ccttacgaac atacgaatcc tgacgtggcg
    21661 tatgtaacct ctttttcggg ttatctgacg catcgcttaa ccggcgaatt taaagataat
    21721 atcgccaact attttggtca gtggccggtg gattataaga cctgggcatg gagcgaagat
    21781 gccgcagtca tggagaagtt taatatcccc cgtcatatgc tgtttgatgt gcaaatgcca
    21841 ggcactgttc ttgggcatat cacacctcaa gccgcgctgg cgacgcattt cccggcagga
    21901 ctgccggttg tttgtaccac cagtgataaa ccggtagaag ctctgggagc cggattactg
    21961 gatgatgaaa ctgcggtaat ttctcttggc acttacatcg cattgatgat gaacggcaaa
    22021 gccttgccga aagatccggt ggcgtactgg ccgattatgt cttctattcc gcaaacattg
    22081 ctgtatgaag gttacggtat tcgcaaaggc atgtggacgg tgagctggct gcgcgacatg
    22141 ttaggcgagt cgttaattca ggatgccagg gcgcaggatc tttcaccgga agatttactc
    22201 aacaagaaag cctctagtgt gccacctggc tgtaatgggc tgatgacggt gctggactgg
    22261 ctgaccaatc cgtgggaacc gtacaaacgc gggattatga tcggctttga ttccagcatg
    22321 gattacgcat ggatatatcg ttcgatattg gaaagcgtgg cgctgacgct gaagaacaat
    22381 tacgacaata tgtgtaatga aatgaatcac tttgcgaaac atgtaatcat tactggcggc
    22441 ggttcgaaca gcgatctgtt tatgcagatt tttgccgacg tgttcaacct tccggcacgc
    22501 cgtaacgcca ttaacggttg tgcaagcctg ggggcagcga ttaatacagg ggtaggtctg
    22561 gggctatacc cggattacgc aacggctgtc gataaaatgg ttcgcgtgaa agatatcttt
    22621 ataccgattg agagcaatgc caaacgctac gacgcgatga ataaaggcat tttcaaagac
    22681 ctaaccaaac atactgatgt gatcctgaaa aaatcgtatg aagtgatgca tggggaattg
    22741 gggaattggg gaatgcggat tcgatccaga gctggtcgaa tgcgtgaata aggtaggccg
    22801 ggcggaaaac tgcccggcct gaacatacct gaatggttat ccccgctggc gcggggaaca
    22861 taagttcgac aacttaaaaa atgataacaa gtccggttta tccccgctga tgcagggaac
    22921 ataataaaaa ctttaactag ttcggtttat ccctgttttt acaaggaa
//
LOCUS       DAESHI010000064        22592 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.064, whole genome shotgun sequence.
ACCESSION   DAESHI010000064 DAESHI010000000
VERSION     DAESHI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22592
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.064"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            235..1341
                     /gene="ompC"
                     /locus_tag="KW011_002542"
     CDS             235..1341
                     /gene="ompC"
                     /locus_tag="KW011_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBH7660846.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1453..2508
                     /gene="apbE"
                     /locus_tag="KW011_002543"
     CDS             1453..2508
                     /gene="apbE"
                     /locus_tag="KW011_002543"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HBH7660847.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2582..3646
                     /gene="ada"
                     /locus_tag="KW011_002544"
     CDS             2582..3646
                     /gene="ada"
                     /locus_tag="KW011_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HBH7660848.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3646..4296
                     /gene="alkB"
                     /locus_tag="KW011_002545"
     CDS             3646..4296
                     /gene="alkB"
                     /locus_tag="KW011_002545"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HBH7660849.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4372..6015
                     /gene="yojI"
                     /locus_tag="KW011_002546"
     CDS             4372..6015
                     /gene="yojI"
                     /locus_tag="KW011_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HBH7660850.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6233..7879
                     /gene="mqo"
                     /locus_tag="KW011_002547"
     CDS             6233..7879
                     /gene="mqo"
                     /locus_tag="KW011_002547"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HBH7660851.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(8188..8676)
                     /gene="eco"
                     /locus_tag="KW011_002548"
     CDS             complement(8188..8676)
                     /gene="eco"
                     /locus_tag="KW011_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HBH7660852.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(8811..8975)
                     /locus_tag="KW011_002549"
     CDS             complement(8811..8975)
                     /locus_tag="KW011_002549"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660853.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            9084..9578
                     /gene="napF"
                     /locus_tag="KW011_002550"
     CDS             9084..9578
                     /gene="napF"
                     /locus_tag="KW011_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HBH7660854.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            9568..9831
                     /gene="napD"
                     /locus_tag="KW011_002551"
     CDS             9568..9831
                     /gene="napD"
                     /locus_tag="KW011_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HBH7660855.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            9828..12314
                     /gene="napA"
                     /locus_tag="KW011_002552"
     CDS             9828..12314
                     /gene="napA"
                     /locus_tag="KW011_002552"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HBH7660856.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            12321..13016
                     /gene="napG"
                     /locus_tag="KW011_002553"
     CDS             12321..13016
                     /gene="napG"
                     /locus_tag="KW011_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HBH7660857.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            13003..13866
                     /gene="napH"
                     /locus_tag="KW011_002554"
     CDS             13003..13866
                     /gene="napH"
                     /locus_tag="KW011_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HBH7660858.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            13863..14312
                     /gene="napB"
                     /locus_tag="KW011_002555"
     CDS             13863..14312
                     /gene="napB"
                     /locus_tag="KW011_002555"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HBH7660859.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            14322..14924
                     /gene="napC"
                     /locus_tag="KW011_002556"
     CDS             14322..14924
                     /gene="napC"
                     /locus_tag="KW011_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HBH7660860.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            14943..15560
                     /gene="ccmA"
                     /locus_tag="KW011_002557"
     CDS             14943..15560
                     /gene="ccmA"
                     /locus_tag="KW011_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HBH7660861.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            15557..16219
                     /gene="ccmB"
                     /locus_tag="KW011_002558"
     CDS             15557..16219
                     /gene="ccmB"
                     /locus_tag="KW011_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HBH7660862.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            16261..16998
                     /gene="ccmC"
                     /locus_tag="KW011_002559"
     CDS             16261..16998
                     /gene="ccmC"
                     /locus_tag="KW011_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HBH7660863.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            16995..17204
                     /gene="ccmD"
                     /locus_tag="KW011_002560"
     CDS             16995..17204
                     /gene="ccmD"
                     /locus_tag="KW011_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HBH7660864.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            17201..17680
                     /gene="ccmE"
                     /locus_tag="KW011_002561"
     CDS             17201..17680
                     /gene="ccmE"
                     /locus_tag="KW011_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HBH7660865.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            17677..19620
                     /gene="ccmF"
                     /locus_tag="KW011_002562"
     CDS             17677..19620
                     /gene="ccmF"
                     /locus_tag="KW011_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HBH7660866.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            19617..20174
                     /gene="dsbE"
                     /locus_tag="KW011_002563"
     CDS             19617..20174
                     /gene="dsbE"
                     /locus_tag="KW011_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HBH7660867.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            20171..21223
                     /gene="ccmH"
                     /locus_tag="KW011_002564"
     CDS             20171..21223
                     /gene="ccmH"
                     /locus_tag="KW011_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HBH7660868.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(21258..21905)
                     /gene="narP"
                     /locus_tag="KW011_002565"
     CDS             complement(21258..21905)
                     /gene="narP"
                     /locus_tag="KW011_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HBH7660869.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            22225..>22592
                     /locus_tag="KW011_002566"
     CDS             22225..>22592
                     /locus_tag="KW011_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBH7660870.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVGLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAVNQTTLNNGVLQVYG"
BASE COUNT         5055 a         5670 c         6428 g         5439 t
ORIGIN      
        1 tgatgttagg tgcttatttc gccattccgc aataatctta aaaagttccc ttgcatttac
       61 attttgaaac atctatagcg ataaatgaaa catcttaaaa gttttagtat catatttgtg
      121 ttggattatt ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc
      181 agtatgcagt ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa
      241 gttaaagtac tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct
      301 gaagtttaca acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac
      361 tatttctctg acaacaagtc agaagacggc gaccagacct atgtacgtct tggtttcaaa
      421 ggcgtaactc aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag
      481 ggcaatacct ctgaagacaa caaagaaaac tcctggaccc gtgtggcatt cgcaggtctg
      541 aaattccagg atgtgggttc tttcgactac ggtcgtaact acggcgttgt ttacgacgta
      601 acttcttgga ccgacgtact gccagaattc ggtggcgaca cctacggttc tgacaacttc
      661 atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ctgacttctt cggtctggtt
      721 gacggcctga actttgctgt tcagtaccag ggtaaaaacg gtagcgtaag cggcgaaggc
      781 atgaccaaca acggtcgtgg tgctctgcgt cagaacggcg acggcgttgg cggatctatc
      841 acttatgatt acgaaggctt cggtatcggt ggtgcgatct ccagctccaa acgtactgat
      901 gatcagaaca gcccgctgta catcggtaac ggcgaccgtg ctgaaaccta caccggtggt
      961 ctgaaatacg acgctaacaa catctacctg gctgctcagt acacccagac ctataacgca
     1021 actcgcgtag gttccctggg ttgggcgaac aaagcacaga actttgaagc tgttgctcag
     1081 taccagttcg acttcggtct gcgtccgtct gtagcatacc tgcagtctaa aggtaaaaac
     1141 ctgggtacca tcgctggtcg taactacgac gacgaagata tcctgaaata tgttgatgtt
     1201 ggtgctacct actacttcaa caaaaacatg tccacctacg ttgactacaa aatcaacctg
     1261 ctggacgaca accagttcac tcgtgacgct ggcatcaaca ctgataacat cgtagctctg
     1321 ggtctggttt accagttcta atctcgattg atatcgaaca aagggcctgc gggccctttt
     1381 ttcattgttt tcagcgtaca aactcatttt tttggtgtac tcttgcgacc gttcgcatga
     1441 ggataatcac gtatggaaat aagctttacc cgcgtggcac tgctggctgc cgcgctcttc
     1501 tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc acgctactga agttaccgtt
     1561 ctagaaggca aaaccatggg taccttctgg cgtgccagca tcccgggcat tgatgccaaa
     1621 cgcagtgccg aacttaaaga aaagattcag acccagctgg acgccgacga tcagctgctt
     1681 tcgacctata aaaaagattc cgcgctgatg cgctttaacg attcgcaaag tttatcgccg
     1741 tggccggtaa gtgaagcgat ggccgatatc gtcaccacct cgctgcgcat tggcacgaag
     1801 accgatggcg cgatggatat aaccgtcggg ccgctggtga atctgtgggg ctttggcccg
     1861 gaacaacagt cggttcaaat tccgagccag gaacagatcg atgcgatgaa agccaaaacc
     1921 ggcttacagc acctgacggt cattaatcag tcgcatcagc aatatctgca aaaagacctg
     1981 ccggatttaa atgtcgatct ctccaccgtc ggtgaaggtt atgcggcgga tcacctggca
     2041 cgcttgatgg agcaggaagg gatttcccgc tatctggtgt cggtgggcgg cgcgctgaac
     2101 agccgtggta tgaacggtga aggcctgccg tggcgggtag cgattcaaaa accaaccgat
     2161 aaagaaaacg cggttcaggc tgtggtggat atcaacggtc atgggattag cacctctggc
     2221 agctaccgta actattacga actggacggc aaacgtcttt cccatgttat cgatccgcaa
     2281 accgggcgtc ccatcgaaca caatctggta tccgtgacgg tgattgctcc gacggcgctg
     2341 gaagccgatg cctgggatac tggcttgatg gtactcgggc cggagaaagc caaagaggtt
     2401 gttcgccggg aagggctggc ggtttatatg atcaccaaag aaggcgatag ctttaaaacc
     2461 tggatgtcac cacagtttaa aagcttcctt atcagcgaaa aaaattaaag cgcaagattg
     2521 ttggtttttg cgtgatggtg accgggcaga ctaaaggcta tccttaacca gggagctgat
     2581 tatgaaaaac gccacatgct taactgacga tcaacgctgg caatctgtct tagcccgcga
     2641 cccgaatgcc gacggcgaat tcgttttcgc cgtgcgcacc acgggtatct tttgccgtcc
     2701 gtcttgccgc gccagacatg ccttgcggga aaatgtctcc ttctacgcaa atgccagcga
     2761 ggcactcgcc gccggatttc gcccctgcaa acgttgtcag ccagacaaag ccaatccccg
     2821 gcaacatcgg ttggataaaa tcacccacgc gtgtcgactg ctggaacagg aaacgcctgt
     2881 aacgctggaa gccttagccg accaggtggc gatgagtcca tttcatctac atcggttgtt
     2941 taaagcgact accggaatga cgcctaaagc ctggcaacag gcctggcgcg ctcgccgttt
     3001 gcgcgaatcg ctggcgaaag gggagagcgt gacgacgtct attcttaacg ccggattccc
     3061 cgacagcagc agttactacc gcaaagccga tgaaacgctg ggcatgacgg ctaaacaatt
     3121 ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg gctgattgtg agctgggtcg
     3181 ttgcctggtg gcagaaagcg agcgggggat ttgcgcgata ttgctgggcg atgatgacgc
     3241 gacattaatc agcgagttgc agcagatgtt tcccgctgcc gacaacgcgc ctgccgatct
     3301 gacgtttcag caacatgtgc gtgaagtgat cgccagcctc aatcaacgcg atacgccgct
     3361 gacgttaccg ctggacattc gcggcactgc ttttcagcaa caagtctggc aggcactgcg
     3421 cacgatacct tgtggtgaaa ccgtcagtta tcagcaactg gctaacgcca tcggcaaacc
     3481 gaaagcggta cgggccgttg ccagcgcctg tgccgccaac aagctggcta tcgtaatacc
     3541 ctgtcatcga gtggtccgtg gtgatggcac actttccggt taccgctggg gcgtgtcgcg
     3601 taaagcgcaa ctgctgcgcc gcgaagctga aaatgaggag aggtaatgtt ggatctgttt
     3661 gccgatgctg aaccgtggca agagccactg gcggctggtg cggtaatttt acggcgtttt
     3721 gcttttaacg ctgcggagca actgatccgc gatattaatg acgttgccag ccagtcgccg
     3781 tttcgccaga tggtcacccc cgggggatat accatgtcgg tggcgatgac caactgtggg
     3841 catctgggct ggacgaccca tcggcaaggt tatctctatt cgcccattga tccgcaaaca
     3901 aataaaccgt ggcccgccat gccacaaagc tttcatgatt tatgtcaacg tgcggctacg
     3961 gcggcgggct atccagattt ccagccagat gcttgtctta tcaaccgcta cgctcctggc
     4021 gcgaaactgt cgctgcatca ggataaagac gaaccggatc tgcgcgcgcc gattgtttct
     4081 gtttctcttg gcttacccgc gatttttcaa tttggtggcc tgaaacgtaa tgatccgctc
     4141 aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg gcggtgaatc gcggctgttt
     4201 tatcacggca ttcaaccgct gaaagcgggg tttcatccac tcaccggcga ctgccgttac
     4261 aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat aagaattatt attgctgtgc
     4321 gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc gggttttctg catggaactt
     4381 cttgtacttg tctggcggca gtatcgctgg ccatttatca gtgtgatggc gctaagcctc
     4441 gccagtgcgg ctttaggcat tggcttaatt gcttttatca atcagcgcct tatcgaaacg
     4501 gcggatacca gtctgctggt gttgccggag tttctgggat tattgctgct gttgatggca
     4561 atcactctcg gatcgcaact ggcgctcacc actttggggc atcacttcgt ttaccgactg
     4621 cgtagtgaat ttatcaagcg gattctcgat acccacgtcg agcgcattga acaactcggt
     4681 agcgcctcgt tgctggcggg attaaccagc gatgtgcgca atatcaccat tgcttttgtg
     4741 cgtctgccgg aactggtgca ggggatcatt ctcactatcg gttcagcggc gtatctgtgg
     4801 atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga tggcggtcac catctggggc
     4861 ggttttgtgc tggtggcgcg ggtgtacaaa catatggcga ccctgcgtga aaccgaagat
     4921 aagctgtaca cggattttca aaccgtactc gaagggcgca aagagctgac tctgaaccgt
     4981 gaacgcgccg agtatgtgtt taacaacctc tacattcctg atgcgcaaga gtatcgccat
     5041 catattatcc gcgcagacac cttccatctt agtgccgtga actggtcaaa catcatgatg
     5101 ctgggcgcta tcggcctggt gttctggatg gcgaacagcc tcggttgggc tgataccaac
     5161 gttgctgcga cctattcgtt gacgctgtta ttcctgcgta cgccgctgct ttcggcggtt
     5221 ggcgcattgc cgacgctgct gacggcgcag gtggcgttta acaagctgaa caaattcgcg
     5281 ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggcgt ttcccaactg gcaaacgctg
     5341 gagctgcgta atgtgacgtt tagctatcag gataacgcgt tttccgttgg gccgattaac
     5401 ctcaccatta aacgtggcga gctgctgttt ctgattggtg gcaacggtag tggaaaatcg
     5461 acgctggcga tgttgctgac gggcttgtac cagccacaaa gcggcgaaat cttgctggat
     5521 ggcaaacctg tcagcggcga acaaccggaa gattatcgca aactgttttc ggcagtgttt
     5581 accgatgtct ggctgtttga tcaactgctg gggccggagg ggcaacccgc taacccgcaa
     5641 ctggttgaga agtggctggc gcagctgaaa atggctcata agcttgagtt aagcaacggg
     5701 cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac gcgtggcgct gttgctggcg
     5761 ctggcagaag aacgcgatat tatcctgctg gatgaatggg cggcggatca ggatccacac
     5821 ttccgtcgtg agttttatca ggtgttgctg ccgctgatgc aggagatggg taaaactatt
     5881 ttcgccatca gtcatgatga tcattacttt atccacgccg accgcctgct ggaaatgcgc
     5941 aatgggcaac ttagcgagct gacgggcgaa gagcgcgatg ccgcttcgcg tgatgccgtt
     6001 gcccggacgg cataatatta cgacgctgac ctggcggact gctgccgcca ggtcaatatt
     6061 ctaaaaatcc cttcatttct aataacccta taattaatca acgaaattat aatgtttcta
     6121 aaattagaat ataatttata aacattattt aaatgttgtt actttagtgt taccgttgat
     6181 gccgcgcaaa tctcacctgc aataaagcga ctaaaagtaa ggcattaaca agatgaaaaa
     6241 agtgactgcc atgctcttct cgatggccgt ggggcttaat gccgtttcga tggcggcaaa
     6301 agcgaaagcg tccgaggagc aggaaactga tgtactgttg attggcggcg gcattatgag
     6361 cgccacgttg gggacctatt tacgcgagct ggagcctgaa tggtcgatga ccatggtgga
     6421 gcgcctggag ggtgtcgcgc aggagagttc gaacggctgg aacaacgccg gaaccgggca
     6481 ttctgcactg atggaactga actacacccc gcaaaacgcc gatggcagta tcagtattga
     6541 aaaagcagtc gccattaacg aagcatttca gatttcccgc cagttctggg cgcaccaggt
     6601 cgagcgcggc gtgctgcgta ctccgcgttc atttatcaat accgttccgc atatgagctt
     6661 tgtctggggc gaggataacg ttaatttcct gcgcgcccgt tacgccgcgt tgcaacaaag
     6721 ctcgctgttt cgcggtatgc gttactctga agatcacgcg cagatcaaag agtgggcacc
     6781 gttagtgatg gaagggcgcg atccgcaaca gaaagtggca gccacgcgta cggaaattgg
     6841 taccgatgtg aactacggcg agatcacccg ccagttaatt gcttccttgc agaagaaatc
     6901 taacttctcg ctgcaactca gcagcgaagt ccgcgcccta aagcgtaatg acgataacac
     6961 ctggaccgtt accgttgccg atctgaaaaa tggcactgca cagaacattc gtgccaaatt
     7021 tgtctttatc ggcgcgggcg gtgcggcgct gaagctgtta caggaatcgg ggattccgga
     7081 agcgaaagac tacgccggtt tcccggtggg cggacagttc cttgtttcgg aaaacccgga
     7141 cgtggttaat caccatctgg cgaaggttta cggtaaagca tccgttggcg caccaccgat
     7201 gtcggttccg catatcgata cccgcgttct ggacggtaaa cgcgtagtgc tgtttgggcc
     7261 atttgccacc ttctcaacca aattcctcaa aaacggttca ttgtgggatc tgatgagttc
     7321 caccaccacc tctaacgtga tgccgatgat gcacgtcggg ctggataatt tcgatctggt
     7381 gaaatacttg gtgagtcagg tgatgttgag tgaagaggat cgttttgaag cgttgaaaga
     7441 gtactatccg caggcgaaaa aagaggactg gcgtttgtgg caagcggggc agcgtgtgca
     7501 gattatcaag cgtgatgccg ataaaggtgg tgtactgcgt ctgggtactg aagtggtcag
     7561 tgaccagcaa gggaccattg ccgcgctgct gggggcatcg ccaggggcgt caaccgctgc
     7621 gccgattatg ttggatctgc tggaaaaagt atttggcgat cgcgtttcca gcccgcaatg
     7681 gcaggctacg ttgaaagcga tcgttccatc ttatggacgc aagctgaacg gtgatgtagc
     7741 ggcaacagaa cgcgagttgc agtacaccag cgaagtgctg ggcctgaaat acgacaagcc
     7801 gcaggcagca gatagtacgc cgaaaccgca gttgaaaccg caacccgttc aaaaagaagt
     7861 ggcggatatt gcgttgtaat gatgtgccac atccggcatg gtatgccgga tgtggcgtaa
     7921 ccgccttatc cgacctacgg ggtggagcac ttcacagcgg tgtaggcctg ataagacgcg
     7981 tcagcgtcgc atcaggcaac cggctcgggc gttagatgtc ggatgcggcg taaccgcctt
     8041 atccaaccta cggggtggag cacttcacag cggtgtaggc ctgataagac gcgccagcgt
     8101 cgcatcaggc aaccggcttg ggcgttagat gtcggatgcg gcgtgaccgc cttatccgac
     8161 ctacggggtg acgcacttca cagcagttta gcgaactacc gcgttgtcaa ttttctcttc
     8221 cgccttccag acgcggtact tcacatctac attgtctggc gtatacacca cgatcggcag
     8281 cttgctgttg taacgcagca ttccagcatc gcccagatac gcggtgacaa atttcttctc
     8341 tttcttgcca tccgggcagg ccatcatcgt ggaaaccggc gagctaactt tatcaaagac
     8401 ataataatca tagccccagc cttccagcgt tttgttttcc agcttcccgc cgagacgatg
     8461 caaattgcaa tcgacttcca gcgtctgacc ggttaacagt tctactttca gggtagattc
     8521 atcttcttgc ggggttaact gaatcacctg acgcttcatc cctttttcag cttgtggata
     8581 aggcgcgatt ttttccagtg gctggacgct ttctgccgcc caggcggaag tggtggcgaa
     8641 agcggcaaac aatactgcag gtagaatggt cttcattttt ttgctttcct tcttcattta
     8701 taggttttca ccgatgatgt cgctgaaggt taacatattc atcagattta ttaatttgct
     8761 ttatgaataa cttaaagaac attcagacaa ttttacctgc tcttttagtg ttaaattctg
     8821 atgagggagc aaaccagcag aaatatctct ggtgaattaa tcgctatata aatatgttta
     8881 taaccatttg aaatgtgagc aaaagcccgt ttttgccacg cttcccgcca ctcttttgat
     8941 cctgctacag gttttacccc gatcggggta tgcatctttg acacatcctt taatatctta
     9001 gcggctataa aaatggctta ttaattatgc ggtttatttg gtcgctctca attttcagag
     9061 cgcgttaatg atggaaggtc aatgtgaaga ttgatgcatc ccgtcggggc atactcactg
     9121 gtcgctggcg caaagccagt aacggtatcc gtccgccctg gtccggtgat gaatctcatt
     9181 ttctgaccca ttgcacccgt tgcgacgcct gtattaatgc ttgtgaaaac aacattctgc
     9241 aacgcggcgc gggcggctat ccgagcgtta atttcaaaaa taacgagtgc agcttctgtt
     9301 acgcctgcgc gcaggcttgc cccgaatcgc tattttctcc gcgccacacc agggcctggg
     9361 atttgcagtt caccattggg gacgcgtgcc tggcgtatca gtcagttgaa tgccgccgct
     9421 gtcaggatag ctgtgagcca atggcaatta tctttcgccc gacgctctcc gggatctacc
     9481 agccgcaact taatagccaa ctttgcaacg gatgtggagc atgtgcagcc agctgtccgg
     9541 tatcagccat aactgcggag tatctccatg cacactaact ggcaagtttg cagcctggtc
     9601 gtgcaggcca aaagcgaacg aatttcagac atcagcaccc aactgaacgc ctttcccggc
     9661 tgtgaagttg ctgtcagcga cgcgccgagc ggtcagttga ttgtggtggt ggaagcagaa
     9721 gacagcgaaa cgctgatcca aaccattgag tcagtacgca acgtagaggg cgtgctggcg
     9781 gtgtcgctgg tttatcacca gcaggaagag caaggtgagg aaacaccatg aaactcagtc
     9841 gtcgtagctt tatgaaagct aacgccgttg cggccgctgc ggcggctgcc ggtctcagcg
     9901 tgccgggcgt tgcccgcgcc gttgttggtc agcaggaagc cattaaatgg gataaagcgc
     9961 cgtgccgttt ctgcggtact ggttgcggcg ttctggtcgg aacgcagcag gggcgtgtgg
    10021 tggcctgtca gggcgacccg gacgcaccgg ttaaccgtgg cctgaactgc attaagggct
    10081 atttcctgcc caagatcatg tacggtaaag accgtttgac gcagccgctg ctgcgtatga
    10141 aaaacggtaa atatgacaaa gaaggcgaat ttaccccaat cacctgggat caggccttcg
    10201 atgtgatgga agagaaattc aaaaccgccc tgaaagaaaa agggccggaa tcgatcggta
    10261 tgttcggttc tggtcagtgg actatctggg aaggttatgc cgcgtccaag ctgtttaaag
    10321 cgggcttccg ttcgaacaac atcgacccga acgcgcgtca ctgtatggcg tcggcagtag
    10381 ttggctttat gcgtaccttt ggtatggatg agccgatggg ctgctatgac gacatcgagc
    10441 aggctgacgc gtttgtgctg tggggcgcaa acatggcgga gatgcacccg atcctctggt
    10501 cgcgcatcac taaccgtcgt ctctctaatc agaacgtcac cgtggcggtg ctttctacct
    10561 accagcatcg tagcttcgag ctggcggata acggcatcat ctttacgcca cagtctgacc
    10621 tggtgatcct gaactacatc gccaactata tcattcaaaa caatgcgata aatcaggact
    10681 tcttcagcaa acacgttaac ctgcgcaaag gggcgacgga catcggctac ggtttacgtc
    10741 cgacccatcc gctggaaaaa gcagcgaaga atccaggttc tgacgcctcc gaaccgatga
    10801 gctttgaaga ttacaaagcc ttcgttgccg agtatacgct ggaaaaaacc gctgaaatga
    10861 ccggcgtgcc gaaagaccag ttagaacaac tggcgcagct gtatgccgat ccgaacaaga
    10921 aagtcatctc ctactggacg atgggcttca accagcatac tcgtggcgtg tgggctaaca
    10981 acctggtcta caacctgcac ctgctgaccg gcaaaatttc ccagccgggt tgcggtccgt
    11041 tctccctgac cgggcaacct tctgcgtgtg gtactgcgcg tgaagtgggc acctttgctc
    11101 accgtctgcc tgcggacatg gtggtgacca acgagaaaca ccgtgatatc tgcgagaaga
    11161 agtggaatat cccgagcggc accattccgg cgaaaatcgg tctgcacgcg gtggcacaag
    11221 accgtgcgct gaaagacggc aaactgaatg tttactggac catgtgtacc aacaacatgc
    11281 aggccgggcc gaacatcaat gaagagcgta tgccgggctg gcgcgatccg cgcaacttca
    11341 tcatcgtctc cgatccgtat ccgacagtca gtgcgctggc cgccgacttg atcctgccga
    11401 ccgcaatgtg ggtagagaaa gagggtgctt acggtaacgc cgaacgccgt actcagttct
    11461 ggcgtcagca ggtacaggca ccgggcgaag cgaaatcgga tctctggcag ttagtccagt
    11521 tctcccgccg cttcaaaact gaagaagtgt ggccggaaga gctgctagcg aagaaaccgg
    11581 aactgcgtgg caaaacgctg tacgaagtgc tttatgccac accggaagtg agcaaattcc
    11641 cggtatccga actggcggaa gatcagctga acgatgaatc ccgcgagctg ggcttctatc
    11701 tgcaaaaagg gctgttcgaa gagtacgcat ggtttggtcg cggtcacggt cacgatctcg
    11761 cgccgttcga tgactaccac aaagcgcgcg gtctgcgctg gccggtggtg aacggtaaag
    11821 aaacgcagtg gcgttacagc gaaggtaacg acccgtacgt gaaagcgggc gaaggctata
    11881 agttctacgg taaaccggat ggtaaagcgg tgatcttcgc gctgccgttc gaaccggcgg
    11941 cggaagcacc ggatgaagag tacgacctgt ggctctctac cggacgcgtt ctggagcact
    12001 ggcacactgg cagtatgact cgccgtgtgc cggaactgca ccgcgccttc ccggaagcgg
    12061 tcttgtttat tcacccgctg gatgcgaaag cgcgcgatct gcgccgtggc gacaaagtga
    12121 aagtggtttc tcgccgtggc gaagtgatct cgattgttga aacgcgcggt cgtaaccgtc
    12181 cgccacaggg cctggtgtac atgccgttct tcgacgccgc acagctggtt aacaaactaa
    12241 cgctggacgc gaccgatccg ctctcgaaag agacggactt caagaagtgc gcagtcaaac
    12301 tggagaaggt gtaagccatt atgtcccggt cagcgaaacc tcaaaatggt cgccgccgct
    12361 ttctgcgcga tgttgttcgc acagcaggcg ggctggctgc cgtgggtgtg gcgctggggt
    12421 tacaacagca aaccgcacgc gcatctggcg tgcggttgcg cccgcccgga gccataaacg
    12481 agaacgcctt tgccagtgcc tgtgtgcgtt gcggtcagtg tgttcaggct tgcccttacg
    12541 acaccttaaa actggcgacg ctggcttctg gcctgtcggc gggcacgcca tattttgtcg
    12601 cacgggatat tccttgcgaa atgtgtgagg acattccgtg cgccaaagtg tgcccgagtg
    12661 gtgcgctgga tcgtgagatc gaatcgatcg acgacgcgcg gatggggctg gcggtgctgg
    12721 tggaccagga aaactgtctc aactttcagg ggctgcgctg cgatgtttgt tatcgcgaat
    12781 gcccgaaaat tgatgaggcc atcaccctgg agctggagcg caacacgcgt accggtaagc
    12841 acgcccgctt tctgccgacg gttcacagcg acgcctgtac cggctgcggt aagtgcgaaa
    12901 aagtgtgcgt gctggaacaa ccggcaatca aggtgttacc gctgtcactg gcgaaagggg
    12961 agttaggtca ccattaccgc ttcggctggc tggaggggaa caatggcaaa tcgtaaacgt
    13021 gacgccgggc gcgaggcgct ggtgaaaaaa ggctggtggc gcagtcaccg ttggctggtg
    13081 ttacgtcgcc tttgtcagtt cttcgtgctg gggatgtttt tgagcggtcc gtggtttggt
    13141 gtgtggatct tacacggcaa ctacagctgt agcctgttat tcgacaccgt tccgctgacc
    13201 gatccgctga tgacgctgca aagtcttgcc agtggtcatc tgcctgccac ggtggcgttg
    13261 accggtgcgg tgataatcac tgtgctctac gccctggcag gaaagcgatt attttgcagc
    13321 tgggtctgcc cgctgaaccc gattaccgac ttagcgaact ggttacgcag gcgctttgac
    13381 ctcaatcagt ctgcgacgat ccctcgccac attcgctacg tgctgttggt ggtcattctg
    13441 ctgggatcag ccttaaccgg cacgctcatc tgggaatgga tcaaccccgt ttccctgatg
    13501 gggcgtagcc tggtaatggg cttcggcagc ggcgcgctgc ttattctcgc gctgttttta
    13561 tttgatctac tggtcgttga acacggctgg tgcgggcaca tttgtccggt tggcgcgctg
    13621 tatggcgtgc tgggtagcaa aggtgtgatt accgttgcgg ccagcgatcg gcagaaatgt
    13681 aaccgctgta tggattgttt tcatgtttgc ccggaaccgc atgtgctacg tgccccggtg
    13741 ctggatgaac aaagcccggt gcaggtcacc agccgcgatt gcatgacgtg cggtcgctgc
    13801 gtggatgtct gttctgagga tgtatttaca ataactacac gatggagttc gggagcgaaa
    13861 tcatgaaaag ccatgacctg aagaaagcgc tgtgtcaatg gacggcgatg ctggccctgg
    13921 tggtaagcgg cgcggtttgg gcggctaatg gcgtcgattt tagccagtcg ccggaagtat
    13981 ccgggacgca ggaaggggcc attcggatgc caaaagagca ggatcggatg ccgctgaact
    14041 atgtgaatca gccgccaatg atcccgcata gcgttgaagg ttatcaggta acgaccaata
    14101 ccaaccgctg cttgcagtgc cacggtgtcg aaagctatcg caccactggc gcgccgcgta
    14161 tcagtcctac tcactttatg gacagcgacg gcaaagtggg cgcggaagtg gctccgcgtc
    14221 gttatttctg tctgcaatgt cacgtaccgc aggccgatac cgcgccaatc gtggggaata
    14281 cctttacccc atcaaaaggt tacgggaaat aagaggttat tatgggaaat tctgaccgta
    14341 agcctggtct gattaagcgc ctgtggaaat ggtggcgtac ccccagccgt ctggcgctgg
    14401 gaacgctgct gttgatcggt tttgtcggcg gcatcgtctt ctggggcggc tttaacaccg
    14461 gaatggaaaa agccaatacc gaagagttct gcattagctg ccacgaaatg cgtaacacgg
    14521 tgtatcagga atacatggat tccgtgcact acaacaaccg tagcggcgtc cgtgcgacct
    14581 gtccggattg tcacgttccg cacgagtttg tgccgaagat gatacgcaag ctcaaagcaa
    14641 gtaaagagct gtatggtaaa atttttggcg ttattgacac gccgcagaaa tttgaagctc
    14701 atcgtctgac aatggcacag aatgagtggc ggcgcatgaa ggacaataac tcgcaggagt
    14761 gccgtaactg tcacaacttc gagtatatgg atacaaccgc ccagaaatcg gttgccgcga
    14821 agatgcatga ccaggcggtg aaagatgggc aaacctgtat tgattgccat aaagggatag
    14881 cgcacaagct gcccgatatg cgtgaagtcg agccaggttt ttaacagggt tattgcgtgg
    14941 gtatgcttga agccagagag ttactttgtg agcgggatga acgaacctta tttagtggct
    15001 tgtcatttac gctgaacgca ggagagtggg tacaaatcac cggtagcaac ggcgcgggga
    15061 agacaacgct tctccgtttg ctgacggggt tgtctcgccc tgacgcaggc gaggttctct
    15121 ggcaagggca gcccttgcat caggtacgcg acagctacca tcaaaacctg ttatggatag
    15181 gccatcagcc ggggatcaaa acccggctga cggcgttaga aaatctgcac ttttatcatc
    15241 gcgatggcga taccgcacaa tgtctggaag ccctggcgca ggccgggctt gccggattcg
    15301 acgatattcc ggtaaatcaa ctttctgccg ggcaacaacg ccgcgtcgct ttagcgcgtc
    15361 tgtggctgac ccgtgccacg ttatggatcc tcgacgagcc ttttaccgcg attgacgtta
    15421 acggcgtcga tcgtctgacc cagcgtatgg cgcagcatac ggagcagggg gggattgtga
    15481 ttctgactac ccaccagccg ctcaacgttg ctgaaagtaa aattcgccgc atttcactga
    15541 cgcaaacgag ggccgcatga tgttctggcg cattttccgt cttgagctgc gtgtagcgtt
    15601 tcgccatagc gccgaaatcg ccaacccgct gtggttcttc ctgattgtaa ttaccctttt
    15661 tccgctcagt atcggtccgg agccgcaact gctggcgcgt attgcaccgg gcattatctg
    15721 ggttgctgcg ctgctttcat ccttgctggc gctggaacga ctgttccgtg acgatttgca
    15781 ggacggcagt cttgaacaat tgatgttgtt gccgttaccc ttgcccgccg ttgtgctggc
    15841 gaaggtgatg gcgcactgga tggtaaccgg tctgccgtta ctcatccttt cgccactggt
    15901 agcaatgcta ctgggaatgg atgtttatgg ctggcaagtg atggcgctga cgctgctgct
    15961 gggaacgcct acgcttggct ttctcggtgc accgggtgtg gcgttgacag tgggacttaa
    16021 gcgcggtggt gtgctgctca gcatactggt gttaccgctg actatcccat tactcatctt
    16081 tgccaccgcc gcgatggacg cggcttccat gcatttgccc gttgacgggt atctggcaat
    16141 tttaggcgcg ttgctggcag gcaccgcgac attaagtcct tttgcgacgg cggcagcgtt
    16201 acgaatcagc attcaataac gctggattac tgatttttgt tcgagtctgg tatcgaaact
    16261 atgtggaaaa cactgcatca actggcgatc ccaccacggc tgtatcaaat ctgtggctgg
    16321 tttataccgt ggctggcaat tgccagtgtg gtcgtgctta ccgtcggctg gatctgggga
    16381 ttcggctttg ctccggctga ttatcagcag ggaaatagct accgcattat ctacctgcat
    16441 gtgcctgcgg cgatctggtc gatgggcatt tatgcatcaa tggcagtggc agcgtttatt
    16501 ggccttgtct ggcagatgaa aatggccaac ctggcggtgg cggcgatggc ccccattggt
    16561 gccgtgttta cctttattgc cctggttacc ggctctgcat ggggaaaacc gatgtggggc
    16621 acctggtggg tatgggatgc acgtctgact tctgaactgg tgctgctgtt tttgtatgtg
    16681 ggtgtgattg ccctgtggca cgccttcgac gaccgccgtc tggcgggccg tgcggcaggt
    16741 atcctggtgc tgattggcgt ggtgaatctg ccgattattc attactccgt ggagtggtgg
    16801 aacaccctgc atcagggatc aacgcggatg cagcaaagta tcgatccggc gatgcgttcg
    16861 ccgctgcgct ggtcgatttt tggcttcctg ctcctgtctg ccacgctgac gctgatgcgg
    16921 atgcgtaatt tgattttgct gatggaaaaa cgccgtccgt gggtgagtga actgatactg
    16981 aaaagaggcc gtaaatgacc cctgcatttg cttcctggaa tgaatttttc gcaatgggcg
    17041 gttacgcctt ttttgtctgg ctggcggtgg tgatgaccgt tattccgctg gtggttttgg
    17101 tcgtgcactc ggtgatgcaa catcgcgcaa ttctgcgtgg tgtggcgcaa cagcgggcgc
    17161 gtgaggcgcg tttacgtgct gcgcaacagc aggaggctgc atgaatattc gccgtaaaaa
    17221 ccgcttgtgg attgcctgtg ccgtgttggc agggctggcg ctgactatcg gtctggtgct
    17281 atatgcgctg cgctcgaata tcgatctctt ttatacgccg ggggaaattc tctacggcaa
    17341 gcgtgaaact cagcaaatgc cggaagtcgg tcagcgtctg cgcgttggcg ggatggtgat
    17401 gctgggtagt gtgcagcgcg atcccaattc gctgaaagtg accttcacta tttacgatgc
    17461 tgaaggctca gtggatgtct cttacgaagg cattttgccg gatctgttcc gtgaagggca
    17521 gggcgttgtg gtgcagggcg agctggaaaa aggcaatcat atcctcgcga aagaagtgct
    17581 ggcgaaacac gacgaaaact acacgccgcc agaagttgag aaagcgatgg aagctaacca
    17641 ccgtcgcccg gcgagtgttt ataaggaccc agcatcatga tgccggaaat tggaaacggg
    17701 ctgctgtgtc tggcgttagg gattgcgctg ctgctgtccg tgtatccgct atggggcgtg
    17761 gcgcgcggag atgcgcgcat gatcgcgtct tcccgcttgt ttgcctggct gctgtttatg
    17821 tctgtggctg gcgcatttct ggtgctggtc aatgctttcg tggtcaacga ctttaccgtc
    17881 acctatgttg ccagctactc caatacccag cttccggtgt ggtatcgcgt ggcggctacc
    17941 tggggcgcgc atgaaggctc gctactgctg tgggtgctgc tgatgagcgg ctggaccttt
    18001 gcggtggcga tttttagtca gcgtattccg ctggatattg tggcccgcgt actggcgata
    18061 atggggatgg tcagcgtcgg ctttttgctg ttcattctct ttacctctaa cccattctca
    18121 cgcacgctgc cgaacttccc gattgaaggg cgcgatctta acccgctgtt acaggacccg
    18181 gggctgatct tccatccgcc tctgctttat atggggtacg tgggtttctc ggtggcgttt
    18241 gcttttgcca ttgcttcttt gctgagcggg cgtctggaca gcacttatgc gcgttttact
    18301 cgcccgtgga cgctggcggc gtggattttc ctgacgctcg gcattgtgct cggttccgca
    18361 tgggcctatt acgaactcgg ctgggggggc tggtggttct gggatccggt agaaaacgcc
    18421 tcgtttatgc cgtggctggt ggggactgcg ctgatgcact cactggcggt cactgaacaa
    18481 cgcgccagct tcaaagcgtg gacattactg ctggcaatca gtgccttctc gttgtgtctg
    18541 ttggggactt tcctcgtgcg ttccggcgtg ctggtatcgg tacacgcgtt tgcgtctgat
    18601 ccggcgcgcg gtatgtttat cctcgccttt atggtgctgg tgattggagg ttcgctgctg
    18661 ctgtttgccg cgcgtggaca caaagttcgc tcacgcgtaa acaatgcgct gtggtcgcgg
    18721 gaatctctgc tgttagcgaa caacgttttg ctggtcgccg cgatgctggt ggtgttgctg
    18781 gggacgctgc tgccgctggt gcacaagcaa ctgggactgg gcagtatttc gattggcgaa
    18841 ccgttcttca acaccatgtt tacctggctg atggtgccgt ttgcgctact gctgggtgtc
    18901 ggtcctctgg tgcgctgggg gcgcgatcgc ccacgtaaaa tccgcaattt attgattatc
    18961 gccttcatct caacgctggt gctgtctctg ctgttgccgt ggctgtttga gagcaaagtt
    19021 gtggcgatga cggtgctcgg cctggcaatg gcctgctgga ttgcggtgct ggcaattgcg
    19081 gaagctgcgc tgcgtatttc acgcggcacg aaaaccacct tcagttattg ggggatggtg
    19141 gctgctcacc tggggctggc agtgacaatt gttggcattg cttttagcca gaactatagc
    19201 gttgagcgtg atgtgcgcat gaagtccggc gatagcgtcg atattcatga atatcgcttc
    19261 accttccgtg atgtcaaaga ggtgactggc ccgaactggc gtggcggtgt ggcgactatc
    19321 ggcgtaacgc gagagggtaa accggaaaca gtgctgtatg cggaaaaacg ttattacaac
    19381 actgccgggt cgatgatgac cgaagcggcg attgacggtg gcatcacgcg tgacctgtac
    19441 gccgcgctcg gtgaagagct ggaaaacggc gcgtgggctg tgcgtcttta ctacaaacca
    19501 tttgttcgct ggatttgggc gggcgggctg atgatggcgt tgggcggact gctgtgtctg
    19561 tttgatcctc gctatcgtaa gcgcgtgagt ccgcaaaaaa ctgcgccgga ggctgtatga
    19621 agcgcaaagt attgttaatt ccgttgatta tcttcctggc gattgccgcg gcgctgctgt
    19681 ggcagctggc gcgtaatgcc gaaggggatg atccgaccaa tctggaatcg gcgctcattg
    19741 gcaagccagt gccgaagttt cgcctcgaat cactggacaa tccggggcag ttttatcagg
    19801 cggatgtgct gactcagggc aaaccagtgc tgcttaacgt ctgggcgacc tggtgtccga
    19861 cctgccgtgc ggaacatcaa tatctgaatc agctttctgc gcagggcatc cgcgtagtcg
    19921 gcatgaacta taaagacgat cgccagaagg caatcagctg gctgaaagag ctgggcaatc
    19981 cttacgcgct aagcctgttt gatggcgatg gcatgttagg gctggatctc ggtgtctatg
    20041 gcgcgccaga aacgttcctt attgacggca acggcatcat tcgctatcgc catgcgggcg
    20101 atctcaatcc tcgcgtctgg gaagaagaga tcaagccgct gtgggagaaa tacagtaagg
    20161 aggccgcaca atgaggtttt tattgggcat gctgatgctg atgatctccg gctcagcgct
    20221 ggcgaccatc gacgtgttgc agtttaaaga tgaagcacag gaacaacagt tccgtcagct
    20281 cactgaagaa ctgcgctgcc cgaaatgcca gaacaacagc attgccgatt ccaactcgat
    20341 gattgccacc gacctgcgcc agaaagtgta tgaactgatg caggaaggta aaagtaaaaa
    20401 agagattgtc gattatatgg tggcgcgtta cggcaacttc gtcacttacg atccgccgtt
    20461 aacgccgctg accgtgctgc tgtgggtgct gccagtagta gctattggca ttggcggttg
    20521 ggtcatatac gcccgctcgc ggcgtcgggt acgcgtggtg ccggaagcgt ttcctgaaca
    20581 aagcgtgccg gaaggtaagc gtgccggata tgtcgcttat ttgccgggca ttgtggtggc
    20641 gttaattgtg gctggcgtca gctactacca gaccggaaat tatcaacagg tgaaaatctg
    20701 gcagcaggcc acggcacagg ctccggcgtt gctggacagg gcgctggatc cgaaagctga
    20761 tccgctcaac gaagaagaga tgtcgcgcct tgcgctgggg atgcgtactc aactgcaaaa
    20821 aaatccggga gatatagacg gctggattat gttgggccgc gttggcatgg cgctgggtaa
    20881 cgccagtatc gccaccgatg catacgctac tgcgtatcgc ctcgatccga aaaacagtga
    20941 tgccgcactg ggttatgctg aagcgttgac acgttcatct gatcccaacg acaaccgcct
    21001 cggcggcgaa ctgctacgtc agctggtgag aacggaccat agcaatatcc gtgtgctaag
    21061 catgtatgcg tttaatgcct ttgagcagca gcgatttggc gaagccgttg ccgcgtggga
    21121 gatgatgttg aaactcttac ctgccaacga tactcgccgt gcggtgattg aacgtagtat
    21181 cgcgcaggcg atgcaacatt tgtcgccgca ggagagtaaa taaaaagagg cccgatggtg
    21241 atgccatcgg gctattttta ttgtgccccg cgttgttgca ggaacagaat ggtggccgcc
    21301 acgcgtgagc ggacattgag tttacgcagc agatttcgaa tatgaacttt tactgtctgc
    21361 tcggaaatat tcaacacaga ggcaatctgt ttatttgaca gcccctgtgc cagctcgtgc
    21421 agaacatcca gctcgcgctc cgtcagcacg ctgaagggat cttcttccgc gccaaacatt
    21481 tcacgttcac gtaagtactg attgacgcgt tcgctaaaga ctttgctgcc tttcgctccg
    21541 gcacgaatcg cttccagcaa cacttccggg tcgctgtctt tcagcagata accgtctgcg
    21601 cctgcgtcta tcagcgcaaa gacatcgctg gaggcatcgg atacggtcag gataataatt
    21661 tgcgcggtaa cgccatccct gcgcaaggca ttgagggtat ccaggccact catacctttc
    21721 atattgagat ccagcaagat cacgtcgata tccagtctat tcgccagatc gatcgcgctc
    21781 gcgccgtcgc ccgcttcggc gaccacttca aagccaggat caagctccag taactgacga
    21841 acaccgcgtc gcataagtgg atgatcatcc acaatcatca cctgaaaagg cgttgcttca
    21901 ggcatagtag tctcctgagg ttttattaga atcattatgt ttgagcgtag cgttggtaac
    21961 gctaccaggc gcgtgaatgt taccccattt tatgtatgga catgtagtga aaactacaac
    22021 cttgccgttc gctttggcat cattttcatt ataaagacgg aattttgcat cagcttctca
    22081 tcaagaaaaa caactaaaaa atcaatcaca atacttgtgc atttctttaa acagttttta
    22141 aaaactgact ccgggtatgg agctatgggt attttctgta cccaatgctt ttaacagcaa
    22201 ttaatttcat aggatgaaag ctcaatgcat caatctggtt ctgtttctct ttgtcgttcc
    22261 gcaatatctg ttctggtggc tacagcgtta tattcaccca tagcattggc atcaacagtt
    22321 gagtatggtg agacagttga tggtgttggc ctggaaaaag atatccagct ggtttatggg
    22381 accgccaata atacgaaaat caatcctggc ggagaacagc atataaaaga atttggtgta
    22441 agtagtaata ctgaaattaa cggcgggtat cagtacattg aaatgaatgg cactgcagaa
    22501 tactcagtat taaatgatgg ttatcaaatt gttcaaatgg gtggcgcggt aaaccagact
    22561 acgctcaata atggagtgct acaggtttat gg
//
LOCUS       DAESHI010000065        22239 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.065, whole genome shotgun sequence.
ACCESSION   DAESHI010000065 DAESHI010000000
VERSION     DAESHI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22239
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.065"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            75..1463
                     /locus_tag="KW011_002567"
     CDS             75..1463
                     /locus_tag="KW011_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH7660871.1"
                     /translation="MSAYLRYIVQKADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPE
                     QRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEG
                     NRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRK
                     DIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTH
                     DRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFIT
                     SSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKILTPELEQRLQK
                     LVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDE
                     FCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIGFSSGIYAMKENDTLHDAYKASDER
                     LYVNKQNKNSRS"
     gene            1506..2009
                     /gene="yeaK"
                     /locus_tag="KW011_002568"
     CDS             1506..2009
                     /gene="yeaK"
                     /locus_tag="KW011_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HBH7660872.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(2010..2114)
                     /locus_tag="KW011_002569"
     CDS             complement(2010..2114)
                     /locus_tag="KW011_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660873.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            2284..2730
                     /locus_tag="KW011_002570"
     CDS             2284..2730
                     /locus_tag="KW011_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBH7660874.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2687..3508)
                     /gene="nimR"
                     /locus_tag="KW011_002571"
     CDS             complement(2687..3508)
                     /gene="nimR"
                     /locus_tag="KW011_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HBH7660875.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3605..4786
                     /gene="nimT"
                     /locus_tag="KW011_002572"
     CDS             3605..4786
                     /gene="nimT"
                     /locus_tag="KW011_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HBH7660876.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            4841..5188
                     /locus_tag="KW011_002573"
     CDS             4841..5188
                     /locus_tag="KW011_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBH7660877.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(5210..5464)
                     /gene="yoaF"
                     /locus_tag="KW011_002574"
     CDS             complement(5210..5464)
                     /gene="yoaF"
                     /locus_tag="KW011_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HBH7660878.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5647..6672
                     /gene="dgcP"
                     /locus_tag="KW011_002575"
     CDS             5647..6672
                     /gene="dgcP"
                     /locus_tag="KW011_002575"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HBH7660879.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            6705..6803
                     /locus_tag="KW011_002576"
     CDS             6705..6803
                     /locus_tag="KW011_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HBH7660880.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6806..6880
                     /gene="yoaJ"
                     /locus_tag="KW011_002577"
     CDS             6806..6880
                     /gene="yoaJ"
                     /locus_tag="KW011_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HBH7660881.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6939..7187)
                     /locus_tag="KW011_002578"
     CDS             complement(6939..7187)
                     /locus_tag="KW011_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBH7660882.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(7335..7517)
                     /locus_tag="KW011_002579"
     CDS             complement(7335..7517)
                     /locus_tag="KW011_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HBH7660883.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(7521..7880)
                     /gene="yeaR"
                     /locus_tag="KW011_002580"
     CDS             complement(7521..7880)
                     /gene="yeaR"
                     /locus_tag="KW011_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HBH7660884.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(8053..8691)
                     /gene="leuE"
                     /locus_tag="KW011_002581"
     CDS             complement(8053..8691)
                     /gene="leuE"
                     /locus_tag="KW011_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HBH7660885.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(8818..9741)
                     /gene="dmlR"
                     /locus_tag="KW011_002582"
     CDS             complement(8818..9741)
                     /gene="dmlR"
                     /locus_tag="KW011_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HBH7660886.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            9844..10929
                     /gene="dmlA"
                     /locus_tag="KW011_002583"
     CDS             9844..10929
                     /gene="dmlA"
                     /locus_tag="KW011_002583"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HBH7660887.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            11180..12790
                     /gene="yeaV"
                     /locus_tag="KW011_002584"
     CDS             11180..12790
                     /gene="yeaV"
                     /locus_tag="KW011_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HBH7660888.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            12822..13946
                     /gene="yeaW"
                     /locus_tag="KW011_002585"
     CDS             12822..13946
                     /gene="yeaW"
                     /locus_tag="KW011_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HBH7660889.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            14002..14967
                     /gene="yeaX"
                     /locus_tag="KW011_002586"
     CDS             14002..14967
                     /gene="yeaX"
                     /locus_tag="KW011_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HBH7660890.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGISITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(15021..16136)
                     /gene="rnd"
                     /locus_tag="KW011_002587"
     CDS             complement(15021..16136)
                     /gene="rnd"
                     /locus_tag="KW011_002587"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HBH7660891.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(16218..17903)
                     /gene="fadD"
                     /locus_tag="KW011_002588"
     CDS             complement(16218..17903)
                     /gene="fadD"
                     /locus_tag="KW011_002588"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HBH7660892.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(18108..18689)
                     /gene="yeaY"
                     /locus_tag="KW011_002589"
     CDS             complement(18108..18689)
                     /gene="yeaY"
                     /locus_tag="KW011_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HBH7660893.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(18729..19424)
                     /gene="tsaB"
                     /locus_tag="KW011_002590"
     CDS             complement(18729..19424)
                     /gene="tsaB"
                     /locus_tag="KW011_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBH7660894.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(19482..21392)
                     /locus_tag="KW011_002591"
     CDS             complement(19482..21392)
                     /locus_tag="KW011_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBH7660895.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDLLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            21524..21868
                     /locus_tag="KW011_002592"
     CDS             21524..21868
                     /locus_tag="KW011_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBH7660896.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5450 a         5698 c         5400 g         5691 t
ORIGIN      
        1 attttatcgc cgcaagtgtc attgtgctga catcttcctt ccttattttt gaacttgtcg
       61 ccagcgacag agcaatgagt gcctatctgc gctatatcgt gcagaaagca gactcctcct
      121 ttctttatga taagtatcag aatcagagta ttgccgcgca tgtgatgcgc gctctcgctg
      181 ctgagcagtc ggaagtgtcg ccagaacagc ggcgcgccat ctgcgaggct tttgagtctg
      241 ccaataatac ccatggctta aacctgactg cccataaata cccgggctta cgcggcacac
      301 tacaaaccgc atccactgac tgcgacacaa ttgtggaagc tgcagcacta ttacccgctt
      361 ttgatcaggc agtggaaggc aaccgccacc aggatgatta cggttcaggt cttgggatgg
      421 ccgaagagaa atttcactat tatctcgatc tcaatgaccg ctatgtctat ttttatgagc
      481 cggttaatgt tgaatacttt gcgatgaata actggtcctt cctgcagtca ggaagtattg
      541 gcatcgatcg caaagatatt gaaaaggtat ttaccgggcg taccgtattg tcgagcattt
      601 accaggatca gcgtactaaa cagaacgtga tgagtttgct gacgccggta tatgtcgcag
      661 ggcagctaaa agggattgtg ctgctggata ttaacaaaaa caatttgcgg aatatctttt
      721 acactcatga ccgccctctc ctctggcgtt ttctcaatgt cacgctaacc gataccgatt
      781 cggggcgcga cattatcatc aaccagagcg aagataatct gttccagtat gtcagttacg
      841 tccatgactt accgggcggc attcgtgtct cgttatccat tgatattctt tactttatca
      901 cgtcttcgtg gaaaagcgtt ctgttctgga ttttgacggc gttaattttg ctgaatatgg
      961 tccgaatgca cttccgttta taccaaaatg tgtcgcgaga aaatattagt gatgcgatga
     1021 ctggactgta taatcgcaaa attttaaccc ctgaactgga gcagcggttg cagaaactgg
     1081 tgcaatccgg ttcttcggtg atgtttattg caattgacat ggacaagtta aagcaaataa
     1141 atgacaccct cggtcatcag gagggggatt tagcgattac gttattagct caggcgatta
     1201 aacaatcgat tcgtaaaagt gattatgcca tccgactcgg tggcgatgaa ttctgcatca
     1261 ttcttgtcga ttcgacgccg caaattgcag cacaactgcc tgaacgtatc gaaaaacgtc
     1321 tgcaacatat cgcgccgcag aaagaaatcg gcttctcttc cggtatttac gcgatgaaag
     1381 aaaacgatac gttacatgat gcgtataaag cttccgatga gcgtttatat gtcaataagc
     1441 agaacaaaaa cagccgttca tgataacctt ctgtggttgt ttgcttgtaa tctcaggagc
     1501 gtgaaatgac tgaaatggct aaaggaagcg tgacgcatca gcggttaatc gctttattat
     1561 cacaagaagg tgctaacttc cgcgtcgtta cccatgaagc ggtaggtaaa tgtgaagcgg
     1621 tgtcagaaat tcgtggcacc gctttaggcc agggagctaa ggcactggta tgtaaagtca
     1681 aaggcaatgg cgtaaatcaa catgttctgg cgatcctcgc tgccgatcaa caggccgatc
     1741 tgagccaact tgccagccat ataggcggat tacgcgcttc gttagccagc cctgctgaag
     1801 tcgatgaact cacaggctgt gtcttcggcg cgatcccccc tttcagcttc catccaaaac
     1861 tcaaactggt tgccgaccct ctactcttcg aacgatttga tgaaatcgcc ttcaatgcag
     1921 ggatgctgga taaatccgtt attttgaaaa ccacggatta tttgcgcatt gcgcaaccag
     1981 aactgctcaa tttccgccgc actgcgtaac tagcctgtcc gttcaataag cagaacggac
     2041 aaaaccagca caacagcaat ggcaaaaaac gatgacgtga taatcagtgt ctcgacgaac
     2101 atttgatcgt tcatagcatg cccccgggag atagctgttt tcacgttatt gttagcgtgc
     2161 acaaatggca gtttgatgac aattcgcctg attttttatg caaaaaacgt aaagattttc
     2221 tacttccttc ctgcagcaaa cgtaaagtaa gcaggcttat tattttttgg caaggaaacc
     2281 acgatgtttg atgtcacttt gctgatcctg ctcgggttag ctgcgctggg ctttatcagt
     2341 cataacacca ctgtcgccgt ttcaattctg gtgttgatca ttgtccgcgt cacaccgtta
     2401 agcacctttt tcccgtggat tgaaaaacag gggctaagta tcgggataat cattctgacg
     2461 attggcgtta tggcccccat cgccagcggg acgctaccgc cctcaacctt gattcactcg
     2521 tttctgaact ggaaatcgct ggtggcaatt gcagtaggcg tgattgtctc ctggctgggt
     2581 gggcgcggcg tgaccttaat gggcagccag ccgcaactgg tcgccgggct gctggtaggc
     2641 actgttttag gcgtggcgct gtttcgcggc gtaccggtcg gcccgcttat tgccgcgggt
     2701 ctggtttcgc tgattgtggg gaaacagtag ttaatcccgc aatatatcgg cctggcgttt
     2761 gccccaggcc tttcttaaac atagtaataa aggccgttgt tgaatcatac cccagcgtat
     2821 gagcaacttt ctgtacagta tcgcctttca ctaatccctg aagtgccata atcagctgta
     2881 actgctgacg ccactggcga aaactcaacc ccgtctcttt tactatcagt cgcgcaagat
     2941 tgcgttcact catcgcaaaa aagccagccc attgccctaa tgacccccac tcgacgggcc
     3001 ctttcgccat catctccacc atcgtgcgga ttttaggatg agaagaaacg ggcaggtgca
     3061 gctgttgttg tggctgctga gggagttcat caaacaggac ctgaattaat ctgcgggtca
     3121 tcggctcagc acgctgagtc gtcgtcctgt ttgccagggt taaaatcaat tcccggcata
     3181 agggcgatat tttcaatgtg caacatgttg tcggcattgt cactgcgctg ggttcgataa
     3241 acagaaaaca gagttcagcg ttagctgtca cttgattgct atgttccacc ccgcccggta
     3301 tccaaaccgc atattgcggc ggcaccatcc acaaagcatt ttccaccgta caggtaattg
     3361 caccatgtaa tgctagtatc agctgtcctt tgcggtgctg atgtacagga ctggttaact
     3421 catccgttcc ggcgtgaata caaaatgcga ccgcggcttc atggtgtcgg tcgggttcat
     3481 agccattgag attcaacctg tgcatcattt tgtccgaatt tagcgataat ttgtcatttt
     3541 agcttgattc aacataacaa taaaaacggt aaggtacagc ctcgtttgta acaatgagaa
     3601 gcatatgacc tgttcaactt cattaagcgg caaaaacagg attgtcctta tcgctggcat
     3661 tctgatgatt gccacaacat tacgcgtcac ctttatcggc gcagcaccgt tactggatac
     3721 gattcgttcc gcttactcgc tgacgacagc gcaaaccggc ttattgacca ccctgccatt
     3781 attggccttt gcgctaatct cacctttggc caccccggta gcgcgacgtt ttggtatgga
     3841 acgtagcctg tttgccgcgt tacttttgat ctgtgctggt atcgcaattc gctctctccc
     3901 ttcgccttac ttattatttg gcggtacagc ggtcattggc ggtgggattg cattaggcaa
     3961 tgtcttactg ccaggattaa ttaaacgcga tttccctcat tccgtcgcca gacttaccgg
     4021 cgcatattcc ctgacaatgg gagctgcagc ggcactggga tcggctatga tagtgccgct
     4081 ggctttgaac ggttttggct ggcaaggcgc gttgctcatg ctgatgtgtt ttcctctgct
     4141 ggctcttttt ttatggctgc cacagtggcg aagccaacaa catgcaaatt tgagtacctc
     4201 gcgcgcctta catactcggg gtatctggcg ttcgccgctt gcctggcagg taacattgtt
     4261 tcttgggatc aactcactga tctattacgt gattattggc tggcttccgg cgatcctcat
     4321 cagtcacggc tatagcgaag cacaggcggg ttcactgcat ggtttgctgc aactagccac
     4381 agcagctccc ggtttgctga tccctctttt cttacatcat gtgaaagatc agcgtggtat
     4441 tgcagcgttc gttgccttga tgtgcgcagt gggcgctgtt gggctctgct ttatgccagc
     4501 gcacgcgatc acctggactc tgcttttcgg ttttggttcc ggcgcaacaa tgatactggg
     4561 gttgacgttc attggtcttc gggcaagttc tgcgcatcag gcggcggcac tctcggggat
     4621 ggcacaatcc gtcgggtatt tgctggcagc ctgtgggccg ccgctaatgg gtaaaataca
     4681 cgatgctaac ggtgactggt ccgtaccact gatgggtgtt gcaatacttt cactactgat
     4741 ggcgattttc ggactttgcg ccgggagaga caaagaaatt cgctaatatc cggtgctata
     4801 gtgacgtgac aaatcatgcg tgaaagggag aacaaacacg atgaatattc agtgcaaacg
     4861 cgtttatgat ccggctgaac agagcgatgg ttatcgcgta ctggtcgacc gcctctggcc
     4921 gcgcggtatc aaaaaaaccg atttagccct tgatgagtgg gataaagaaa tcacgccgtc
     4981 aacggaactg cgcaaagcct ttcacggcga agtcgtcgat tttgcaacct ttcgcgagca
     5041 atatcttgca gaactggcgc aacacgagca agaaggaaag cggctggcgg acatcgccaa
     5101 aaaacagccg ctgaccctgc tctacgcagc aaaaaacacc acgcagaacc atgcgctggt
     5161 gctggccgac tggctacgta gcttgtgatt ttagtacagc atccggcggt tatttttcac
     5221 cagccggatg gtcccgtcgc cacaatgccc attcatcaat cgtttcaccg cccggtaatt
     5281 tgcaattgtt gctgacccct tgcgctgtcg acactggaat gagcgtcccg cccttctgct
     5341 ggcaatagac cgacgccgga tttgccatac caatctgcgg cggtttaggt acttctggct
     5401 gagaagaggt tgaacaacca gccaggacca gcaagcaagg caaagcaaaa ctgataattt
     5461 tcatttattg atctcactca tttatctacg cttagagtat cgcggtatcg ttttgttttg
     5521 cagcactatt tttattacat tcactcaaaa catattacgt cttgtttcat ctttgttggt
     5581 gatgttttat catgcctgca aagattaaat aatcagcatt tacccgccgt atcctggagt
     5641 tgtttcgtgt cagatcagat tatcgcccgc gtctcgcaat cccttgccaa agaacagtca
     5701 ctggaaagtc tggtccgaca gcttctggag atgctggaaa tggtcactga tatggaatca
     5761 acctacctga ccaaagtgga tgtcgaagcg cgcctgcagc atattatgtt tgcccgtaac
     5821 agccagaaaa tgcacatccc ggagaatttt accgtctcgt gggattactc gttatgcaaa
     5881 cgcgccattg atgaaaactg ctttttcagc gatgaagtcc ctgaccgttg gggcgactgt
     5941 attgcggcac gcaatcttgg catctccaca tttctgagca caccaattca cttaccggat
     6001 ggatcattct atggcacgct ttgcgccgcc agcagtgaga agcgccagtg gagtgaacgc
     6061 gcggaacagg ttttacagtt attcgccgga ctgattgcac aatatattca aaaagaggca
     6121 ctggttgaac agttgcgcga agccaatgct gcgctgattg cgcaatcgta taccgactcg
     6181 ttaaccgggt tgccgaatcc gcgggcgatt tttgaaaatc tgacgacgct gttttctctc
     6241 gcccggcatc ttaaccataa gataatgatc gcgtttatcg atctggataa cttcaaatta
     6301 atcaatgatc gttttggtca taatagtggc gatctgtttc tcattcaggt tggcgagcgc
     6361 cttaatacgc tccagcaaaa tggcgaagtt attggtcgtc tcggcggtga tgagtttttg
     6421 gttgtttcac taaacaacga gaatgcggat atttcgtcgc tgcgagaacg cattcagcag
     6481 caaatacgtg gagaatatca cttaggtgat gttgatttgt attatcccgg tgccagtctt
     6541 ggcatagtag aagtcgatcc tgaaacaacc gatgcagaca gtgccctgca tgctgccgat
     6601 attgcgatgt atcaggagaa aaaacacaaa cagaaaacac cttttgtcgc gcattcagcg
     6661 ccacattgct gaggcgtatt cacatccttt tgattggtga taacatgcga atcggtatta
     6721 tttttccggt tgtaatcttt attacagcgg tcgtattttt agcatggttt tttattggcg
     6781 gctatgctgc cccgggggca taaagatgaa aaaaacaacg attattatga tgggtgtggc
     6841 gattattgtc gtactcggca ctgagctggg atggtggtaa cgtcacctct aaaaaatagc
     6901 aaaggctgcc tgtgtgcagc ctttgtgcaa tttaagcgtt aacttttaat cttcctgtag
     6961 ataaatagca cgacaatcgc accaataacg gcaaccacga agctgccaaa attgaagcca
     7021 tcgactttac caaagccaaa cagcgtgctg atccatccgc cgactacggc accgactatc
     7081 cccagcagga tagtcataaa gaatccacca ccatctttac ctggcatgat ccacttcgcc
     7141 agaataccgg caataagccc aaaaataatc catgacagaa tgcccattgt ttcctcactt
     7201 atctgttttg cattagcggg ttagtcgctg ataaaaagca tagcacaaca tcgggagggc
     7261 aagatttgtg acgagcatca cggaggattt ttttgcgatg gcgcagaaat tgcgccatca
     7321 acgatcagtg ataattacca accacaaaca tcatgttcgt tttccgtgtc ataagaacgc
     7381 acggtattca ccagatcttt tatcacttca gccgccactt ctggcaccag caaagtcatc
     7441 ggcgtctctg tttcataatc gacagaaacg ccattactgt tattggtgac ggtcacggta
     7501 tacgttgctt tgcccatgat tcatttcccg ttatgaatga ctttccgttg ttgcgcacct
     7561 tccatcagga cttcaggagc cacgaagaag tcaatgttga aataagtatc gtcagtcatg
     7621 gcttcaatgt tgtgccactt ttctggaggg aacacctcaa actgccccgc ttcgataagg
     7681 atcacctgat caggctctgc actgtgttca tcagcgtagc cgagatattt gaccgcccca
     7741 tgcataacgg aaaggcgtgg gtaaaccccc gggcgcgttc ctttatcaag atgacgttcg
     7801 aatattccgg caggcgcagt ttgtttattc cagaaaggcg ttgagcgcgt atgaatataa
     7861 ttctgtggga tttgcagcat ccttttccct ccttcggtga atgcgctgaa aacggtttat
     7921 tccagccgtt tcagggtacg cctgataatt tgcattttaa ataccattta ttggttactt
     7981 tttagcacca tatcagagaa aaatcaggga ggattacaag tgggaagccc gcgacaacgc
     8041 gggctgaatg tatcaggatt gcagcgtcgc cagtcgggcg gcgaaaccca cgaacatcaa
     8101 accaatcagt gagttgccaa ctttagccag tttctttttg gtacgtatgt actgcgtgac
     8161 aaaagcacca gaaataatca ggaagctcaa atagcagaaa ctcaccagtt ccagcgtcgt
     8221 cgccagaata aagaatgaaa ttcccgtatg tggggcatta acatcgataa actgtacgaa
     8281 aaacgacaca tagaacaaaa tggctttcgg attagtcagg ctcaaaatta acgcgcgttt
     8341 aaaaatggcc ccgtattggg gctcatcgga tttggcctcg ttatttttac ccttcagggt
     8401 cgcgtagaga attttactcc ccagatagag caaataaaac gcaccaagat aacgtacaat
     8461 gttgaataat atcggggtgg tcttaattaa tgtcgccact ccagcccatg ccagaaacat
     8521 caatactgca tcgccaataa atacaccgca ggccgcaaga taaccgcctt tcataccgct
     8581 actgacgcta tttttgagta caaacagggt atttggccct ggcaccaaca caataaaaat
     8641 ggccccaacc agataggtcc agtaattcag aaccccgtat tcagcgaaca cattaacctc
     8701 tttaattatc tttcgatcat gcgcgattaa aggtgaatat gctaaccaat ctgtagcggc
     8761 ttagaaagga gaaaatcagg ttttaacccg atatcaaccc gataattgaa taattaatca
     8821 ggcatgctcc agtgaaaaat tcgggtagtg ctcggcaaaa tactggcgta aaaattctat
     8881 cgttatccgc actttcgctg acgtcgccag ccttgaaaca taaacggccc agacgttcgc
     8941 tggctggtaa tattccggta gcacttgcac taaatgacca ctggcaatgt tttcgctaac
     9001 atcccaccag gagcgcagag caatcccctg cccatcgaga caccactgat ggacaatttc
     9061 tccatgatta gacgacagtg ggccggtgac tttaatcgca tggtggcctt ctttattgcg
     9121 taattgccag acaccaaaag gatgatcgcg ctctttaatg accaggcacg gcaatgcgga
     9181 aagatcggtt aaatgtttcg gcgcgccatg ttgcgcaata aattccggcg acgcgcagag
     9241 aatgcggtaa ttagtcgcca gtttgcgggc gattaaatta ggggcgatgt catcgccaat
     9301 gcgaatatcg agatcaacac cttcattgac caaatcgacc aaccggtctt ccacatcaaa
     9361 acgtaattcc agttgcggat acgccttagc cagcgctgat aacgccggag ccaccacctg
     9421 tcgtccaaaa ccaaagctgc tgataatacg cagcatcccc tgcggcacct gacgcacgtc
     9481 agaaagttcg tccatcatct gaccgacatc ctgcaaaatc cgctgcgccc attcataaat
     9541 ccgctctcct tcttcggtaa tggtgacgcg gcgggtggtg cggtgtaaca acaccacgtt
     9601 tagcgtttgc tccagcaagg cgatgcgctt gctgacgaac gccggtgaaa cgcccagttc
     9661 ttccgccacg gcggcaaaac cagcccggcg agccaccagc ataaagacgc gcaaatcgtt
     9721 cagcagcggt aaattattca tgattcgtgt tttatgtttc accagttacg gggattaatt
     9781 ccttttcagt caattatagg atggtgatgt tgtcaatttt gatggtcagg aagtgagaac
     9841 ccaatgatga aaacgatgcg tattgctgcg atcccgggag atgggattgg caaagaagtc
     9901 cttcctgaag ggattcgcgt attacaggct gccgctgagc gctggggctt cgacttgagt
     9961 tttgagcaaa tggagtgggc gagctgcgag tattacagcc atcacggtaa aatgatgccg
    10021 gacgactggc atgagcaact tagccgtttc gacgccatct attttggtgc cgtcggctgg
    10081 ccggataccg ttccggacca tatttcgttg tggggttcgc tgctgaaatt tcgtcgtgaa
    10141 ttcgaccagt acgtcaacct gcgcccggtt cgtctctttc ctggcgttcc ctgcccgctg
    10201 gcgggaaaac agcctggcga catcgatttt tacgtggtca gggaaaacac cgaaggcgaa
    10261 tattcctcgc tcggcggtag agtgaatgaa ggtacagagc atgaagtcgt cattcaggaa
    10321 tcggtattta cgcgtcgtgg tgtcgatcgc attttgcgtt atgccttcga acttgcgcaa
    10381 agccgcccac gtaagacgct aacttctgcc actaaatcaa acggtttagc catcagcatg
    10441 ccgtactggg atgagcgagt ggaagcaatg gccgagaatt acccggagat ccgctgggac
    10501 aagcagcata ttgatattct ctgcgcgcgt tttgtgatgc agccggaacg cttcgatgtg
    10561 gtggtggcgt ccaatttgtt tggcgatatc ctttccgatc ttggcccggc ctgcaccggc
    10621 accattggca ttgccccatc cgccaacctg aatccggaac gcactttccc gtcgctcttc
    10681 gagcctgtcc acggttccgc gccggatatc tacgggaaaa atattgctaa ccctatcgcc
    10741 acaatttggg ccggtgcgat gatgctcgat tttctcggca atggcgatga gcgtttccag
    10801 caagcgcata acggtattct ggcagcgatt gaagaagtga ttgctcacgg gccgaaaaca
    10861 ccggatatga aaggcagtgc caccacgcca caggttgccg acgcgatttg caaaattatt
    10921 ttgcgttaag gtcaaaccag tttatttgaa ccgcgtcact gacgcggttt ttttattcgt
    10981 tctttgcagt taataacctg cgtcatttca ccttttattg tttccgtttc gtgttttatg
    11041 gctttccgta ttcttaattg tttaatttat gtaacatgca aataatcaat ttgttaaaca
    11101 caacatacaa ctcattaccc tgcaaataat tacaactggc aaaagccaga gacgggcttt
    11161 tatgcccagg agatgactca tgatgagcaa tgttaagaaa aaagatgtgc cgctgataag
    11221 catcagcctg gtggccattc ttttcatcgc agctgcatta agccttttcc cacaacaatc
    11281 ggccgacgcg gccaacacaa tatacacttt tgttacgcgt acgttaggtt ccgccgtaca
    11341 ggtattggtt ttgctggcaa tgggactggt gatttattta gccaccagta aatacggcaa
    11401 tattcgtctt ggcgaaggaa aaccggaata cagcacgctc tcctggctgt ttatgtttat
    11461 ttgtgccggt ttaggttctt ctacgcttta ttggggggtt gctgaatggg cctattatta
    11521 tcaaacgcct ggattaaata tcgcaccacg ttcacaacag gcactcgaat ttagcgtccc
    11581 ttactccttc ttccactggg gcatcagcac ctgggcaact tatacgctgg cctcattaat
    11641 catggcttat cactttcatg tgcgtaaaaa caaaggtctg agtctttccg gcattattgc
    11701 cgcgattacc ggcgttcgcc cgcaaggccc atggggaaaa ctggttgatt tgatgtttct
    11761 gatcgccact gtcggcgcac tgactatttc ccttgttgtc accgcagcaa cctttactcg
    11821 tggactttcc gcgctgaccg gtttacccga taacttcacc gtgcaggcat ttgtgatcct
    11881 gctttccggc ggcatttttt gcctaagctc gtggattggt atcaacaacg gtttgcaacg
    11941 tctgagcaaa atggttggct ggggcgcgtt cctgctgcca ttactggtgc tgattgtcgg
    12001 tccaaccgaa tttattacca acagcatcat caatgccatc ggcctgacca cgcaaaactt
    12061 cctgcaaatg agcttattca ccgatccgct tggcgatggt tcatttaccc gcaactggac
    12121 cgttttctac tggctgtggt ggatctcata cacccctggc gtagcaatgt ttgtcacccg
    12181 cgtttcccgc ggtcgtaaga ttaaagaagt tatctgggga ctgatcctcg gcagcaccgt
    12241 cggttgctgg ttcttcttcg gcgtaatgga aagctatgcc attcatcagt ttatcaatgg
    12301 cgtaatcaac gtcccacagg tgctggaaac actgggcggc gaaacagctg tacagcaagt
    12361 tctgatgtcg ttgccagccg gtaaattgtt cctcgccgca tacctgggcg tgatgattat
    12421 tttccttgcc tcgcatatgg atgcagtggc ctacaccatg gcggcgacca gtacgcgtaa
    12481 tctccaggaa ggtgacgatc ctgaccgtgg gctgcgtctt ttctggtgcg tggtgatcac
    12541 tctgatcccg ctttccatct tgtttaccgg tgcttcgctg gaaacgatga aaaccaccgt
    12601 cgtgctcaca gcccttccct tcctcgtcat tttactggtg aaagtcggcg gatttattcg
    12661 ctggctgaaa caggattatg ccgacattcc ggctcatcaa gttgaacatt atctcccgca
    12721 gacaccggtt gaagccctgg aaaaaacacc agtgctccct gcgggaaccg tattcaaagg
    12781 cgacaactga gcgccatcat cctaacgata aaggtatccc tatgagcaat ctgagccctg
    12841 actttgtact acccgaaaat ttttgcgcta acccgcaaga ggcgtggacc attcctgccc
    12901 gtttttatac cgatcagaac gcgtttgaac acgaaaaaga gaacgtcttc gccaaaagct
    12961 ggatttgcgt cgctcacagc agcgaactgg cgaatgccaa tgattatgtg acgcgtgaga
    13021 tcattggcga aagcatcgtg ctggtacgcg gtcgtgataa ggttttgcgc gcgttctata
    13081 acgtgtgtcc gcaccgtggt catcagttgt tgagcggtga aggaaaagca aaaaatgtga
    13141 ttacctgccc atatcacgca tgggcattca aactcgatgg caatctggcc catgcacgta
    13201 actgcgaaaa cgtcgccaat ttcgatagcg acaaagcgca actggttccg gtgcgtctgg
    13261 aagaatatgc cggattcgtc ttcatcaaca tggaccccaa cgccaccagc gtagaagatc
    13321 agttacccgg tctgggcgcg aaagtgctgg aagcctgccc ggaagtccac gatctgaaac
    13381 tggcggcccg ctttaccacc cgcacacctg ccaactggaa gaacattgtc gataactatc
    13441 tcgagtgcta tcactgtggt ccggcgcatc caggtttctc cgactccgta caggttgatc
    13501 gctactggca caccatgcac ggtaactgga cgctgcaata cggtttcgcc aaaccgtccg
    13561 aacagtcgtt taaatttgaa gagggtacgg atgcggcatt ccacggtttc tggctgtggc
    13621 cgtgcacgat gctgaacgtc accccgatca aagggatgat gacggtcatt tatgaattcc
    13681 cggtggattc tgaaactacc ctgcaaaact acgatattta cttcaccaat gaagagttaa
    13741 ccgacgagca aaaatcgctg attgagtggt atcgcgatgt gttccgtccg gaagatttac
    13801 gtctggttga aagcgtacag aaagggctga aatcgcgtgg ctatcgtggt caggggcgca
    13861 tcatggccga cagtagcggt agtggcattt ccgaacatgg tatcgcccat ttccataatc
    13921 tgctggcgca ggtgtttaag gactaatgac atcggcggcg gtattttccg ccgctgggct
    13981 gatttttgat ggagtacagc aatgtcagac tatcaaatgt ttgaagtaca ggtgagtcag
    14041 gtggaaccgc ttaccgaaca ggtgaaacgc ttcacgctgg tggcaaccga tggcaaaccg
    14101 ctgcctgcgt tcaccggagg aagtcacatc attgtgcaga tgagcgatgg tgataaccag
    14161 tacagcaatg cgtattcact actgagttcg ccgcatgaca cctcttgtta tcagattgcc
    14221 gttcggctgg aggaaaactc gcgcggcggt tcccgctttt tgcatcagca ggtaaaagtg
    14281 ggcgatcggt taacgatttc aacgcctaat aacctgtttg cgctaattcc ctcagccaga
    14341 aagcatctgt ttatcgcggg cggtattagt atcacccctt tcctgtcgca catggcagag
    14401 ttgcaacaca gcgacgtcga ctggcagcta cattactgct cgcgaaatcc agaaagttgc
    14461 gcatttcgtg atgagctagt ccagcatccg caggctgaga aagtccattt gcatcattca
    14521 tcaaccggaa cacgactgga attagcgcga ttattggcgg atatcgaacc tggcacacac
    14581 gtttatacct gtggccccga ggcgctaaat gaagcggtaa gaagtgaagc tgcgcgtctg
    14641 gacatcgccg ccgatacgct gcactttgag caatttgcta tcgaagacaa aaccggcgat
    14701 gcctttaccc tggtgcttgc ccgttccgga aaagagtttg tggtgccgga agagatgact
    14761 attttgcagg ttattgaaaa taataaagcc gcgaaagtgg aatgtttatg tcgtgaaggg
    14821 gtatgcggaa cctgcgaaac agcaatactg gaaggtgaag ctgaccatcg ggatcagtat
    14881 tttagcgatg aagagcgtgc cagccagcaa agtatgttga tctgttgttc gcgtgcgaag
    14941 ggtaaacgcc tggtgttgga tttgtagttt gccaaagccg gatgtggcgc tgagcgcgcc
    15001 cagtccggct tcggaagatt ttactgcgga tattcctgca ataaattgtg taatgcttcc
    15061 gccatcagct caccacgcca gccggaaatc agctccggca aattgttctg cggtttcagt
    15121 ttccagtgcc agttcagcag ttggttgatt tgccgacgcg atgccagcaa ttcggcgctg
    15181 atcttatgcg tttcgctcac gtcagtaatc agcgacttaa tcgctttaaa cgctttacga
    15241 taacccggca tgtccatcag gttaagcatc ggctgcggta aggcctcttc cggcaatgtc
    15301 tgcgcttttt ccaccagcgc cagcagcgtt ttaccgtgaa agcggatttc gctaccggat
    15361 aaacccaggc tgtccagttc gcctaaactt cccggcatat aacgcgcgac cgaccacaaa
    15421 tgctcttcac gcacgacaaa gttcaccgcc agatcacgct cacgagcctt gcgcagtcgc
    15481 cagtcggcta acagttgcag acaggccagt tggcgtgtgc gtaattgcca ggcattggtg
    15541 atatcacgcc aggcatcttc cggcgcaacg acttcctgac gacgcatttg catcaggcgg
    15601 cattcatcca gcgccgcagg tagccagccg gaggcctccg tttctaccat aagcttggcg
    15661 gtgatcggta acagatacca gacatccgcc gctgcgtatt cacactgacg ttcggtcagc
    15721 ggtctggcca gccagtcggt gcgcgattca ctcttgtcca gcgtaacgcc ggaatactct
    15781 tccaccatgg aagcgaaacc ccatgacatc gggcgtccgc agaaggcagc aaggatttgc
    15841 gtgtcaatca ggggttgtgg taattcgcca aagacattga ggaacacttc cagatcttca
    15901 ctgcctgcat ggagaaattt tgtgatggac ggatcgcgca ggatcgcttt cagcggtgac
    15961 cagtcggtga tcccgagagg atcaattagc gccagatgct caccatcgaa aagttgaatc
    16021 aaccccagct gcgggtaata agtgcgcgta cgaacaaatt cagtatcctg ggctatcgcc
    16081 ggaaagacac ggacggcttc acacaaagaa gccagcgcat cgtccgtggt aatcatttgg
    16141 taattcaaat tgtattctct ttagtgggcg tcaaaaaaaa cgccggatta accggcgtct
    16201 gacgactgac ttaacgctca ggctttattg tccactttgc cgcgcgcttc gtcacgtaat
    16261 tctcgtcgca aaatttttcc gacgttagat ttcggtaact catcacgaaa ctccaccagc
    16321 ttcggtactt tgtagcccgt gagctgacgg cagcaaaagg tcaccagtga ctcttcggta
    16381 agcgatggat cttttttcac tacgaagatt ttcaccgctt caccactgga gccggaaggt
    16441 acgccaacag ccgcgacttc ctgtacgcca ggatgctgca tgacgacatc ttcaatctcg
    16501 ttgggataga cgttaaaacc ggaaaccaga atcatgtctt ttttacgatc gacaatgcgc
    16561 aggaaccctt cttcatccat caccgcgatg tcgccggtgt gtaaccagcc atttttgatg
    16621 atctcatcgg tagcatcggg acgctgccag taacccagca tcacctgcgg tcctttgaca
    16681 caaagctcac ccggttgacc cggtggtact tcattatcgt cgtcatccac cagtttggct
    16741 tccgtcgacg gcaccggcaa accgatgcta ccactatgat aatcaatatc atatgggtta
    16801 acgctgacca gcggcgcaca ctcggtaaga ccatagcctt ccagcagata ctgtcctgtc
    16861 agtttcaccc aacgctctgc caccacttgc tgcactggca tcccaccgcc tgcggaaaga
    16921 tgcagactgg agaaatccag ctgctggaac tctttattgt tcagcaacgc attgaacaag
    16981 gtgttaacgc cagtgatagc ggtaaacgga tatttcgcta actcttttac caaccctgga
    17041 atatcgcgcg ggttagtgat aagcaggttc tgcccaccca gttcgataaa cagcaggcag
    17101 ttaatggtca gggcaaaaat gtgatacagc ggcagtgccg ttactaccag ttctttacct
    17161 ggatgcagca acggcccata ggtggcgttg acctgttcca ggttcgccag catattgcgg
    17221 tgagtcagca tcgcgccttt cgccacacct gtggtgccgc cggtgtattg caggaaggct
    17281 aaatcttccg gcaccagttc gggtttgacg tactgcatcc ggtagccgtt atgcagtgcg
    17341 ctacgaaatg aaatggcatc tggcagatgg tatttcggca ccaaacgctt gatgtattta
    17401 acaacgaaat tgactaccgt gccttttgcc gtagatagct gatcgcccat acgggtcaga
    17461 attacgtgct gaacggcggt tttatcaacc actttttcca gtgtgtgagc aaagttagac
    17521 acgataacaa tcgccgatgc gccgctatcg ttaagctgat gctcaagctc acgcggggta
    17581 tacaacgggt taacgtttac gacgatcatc ccggcacgca aaatgccaaa cagcgccacc
    17641 ggatattgca ataaattagg catcatcaac gcaacgcgat cgcctttctt cagccccaac
    17701 ccttgttgca aataagcggc aaacgcgcga ctgcgttctt ccagcttgcg gaaggtcatt
    17761 acctccccca tattcacaaa cgcaggctga tcggcgtagc ggacgaccga ctgctcaaac
    17821 atatctatca gagattgata acggtcaggg ttgatctccg tcggaacatc cgcgggataa
    17881 cggttaagcc aaaccttctt caattcttca cctctaaaat gcgtgttcgt cgtcatcgca
    17941 accccaagtg ataaacatgt tgttaacata acattaactc atcttaccag cttgataatt
    18001 agacaacgaa aaggttgcga agcgcgtcac tatttatttt tatagttgcc gtatgaacgc
    18061 agaaacagcg gaccagccgc tgtttctttt tctttaaaaa caacttgtta ctcggttaca
    18121 attgtttcaa ctcttgcagg gccaggatta taccaacccc atccgccgta accatagggc
    18181 cagccgcgtc caccataaaa ccatggatca attggctgcg gcggcataat cacctgttga
    18241 gtgatatgcc agcgtttgta acctgttacc tgcataacca taaatttata tggtgcatta
    18301 tttactttgc catccactgt gccagtgatt ggcccgacaa ccgtcaccag ttgtccacga
    18361 aaatcgaccg gatcaagaaa accgttcaca tcggcataaa tgcgcccgcg tgaaggttct
    18421 cccaaggttg gtcttgctcc gctgtccagc ggtacggtag caatttccag gcgggttttc
    18481 ccttgctggt tttgtaccac aaccactttg ccgccaaagc gcgcctcctg accaacatat
    18541 aaatctggcg cattcatcac gcgtaccaga tcttgctgtg gtgttggact gctgccttta
    18601 atggcgtccg gcacagtgac acaaccgctc agcattagcg caaacgtacc tgccagtatg
    18661 cctttgataa cattcttttg aaccgccatg atgcgactcc tttttttctc agggcatact
    18721 cttaagattc attctttgcc cggaagtttc ttccatgcga cgttgttacg taaataaacc
    18781 ggttcggcat gttccaccgc caccgtttta ccctcggcaa acatctgaca cgcaatcggc
    18841 agcatatctt ccgccgctgg cagtaaaact tctccgtcgc gcaaaaccag cccgctctct
    18901 ttacggagat ccggccaggc ttgccagccc gtccctaccg ttacccattc gccggaaagc
    18961 tgttgcattc gttcatgaac gagttcgggt ttgagtacgg cttcggtttc ttcaccgcgc
    19021 cagataccgt tttcatcacg ctgatattcg gcccagtaaa cttcgcccat tcgcgcgtca
    19081 atggctgcca gcacgcgggt tgcgccgttt ttgcgccacg ccccctgcgc catcgtcatt
    19141 agcgtagaga caccaatcat cggtaactcc gcgccaagcg ccagcccttg cgcaatacca
    19201 ataccaatgc gcacgccagt aaagctaccg gggccgcgcc cgtaagccag agcgtttatg
    19261 tcagtcaggg aagttccgct ggtggtcagg atatcctgca ccatcggtaa gattcgttga
    19321 gtatgttcac gagggcaaag ctcaaaatga gcgttgacat taccgtcgtt ccacagagcg
    19381 acagagcaga cctctgtcgc ggtatcgata gccagaattc gcatgggtct tcgtgcttag
    19441 atcaataaaa aggcgcgcat cataccatac tccgtaacaa attacctgga ggatggtatc
    19501 gcaaggaaac gaaccgcacg ggcaatgtca cgggtgcgtg gcgcgggcgg cagactggcg
    19561 agaaacgtcg cgccgtaagg acgcatcacc agccgattgt cacaaattac cagcacgccg
    19621 cgatcgtcgg cgtcgcgaat cagtcgccct actccctgct tgagagtaat gaccgcatcc
    19681 ggcagctgca cttcatcgaa cgggtcgcca ccgcgcaaac gacaatcttc catgcgcgct
    19741 tttaacagta gatcatccgg cgaggtaaac ggcaatttgt cgataattac caatgacaat
    19801 gtatcgccac gcacgtccac cccttcccag aaactactgg ttgccacaag aagcgcgtta
    19861 ccggcgctga caaattgctg caacagttgc cctttgctgg tttccccctg caacaaaacg
    19921 ggaagcgtca tggtagcgcg gaactgctcg gccagatcgc gcatcatggc atgcgaggta
    19981 caaagcataa aacaacgacc gttgttagct tcgatgatcg gtcgcagcat tgccgccagt
    20041 tgccgtgcac aacccggttg attggtttgc ggcaggttgc gcggcacaca gagtaacgcc
    20101 tggcggctgt aatcaaacgg gcttggcaat agcaacgact ccgcctgttc tatcccaagc
    20161 cgcgaggtga aatgatgcag atcgtcgttc accgacagcg ttgctgaggt gaagatccag
    20221 ctaccgggct tttgcgccat taactctttg aatttatccg ccacgctgag cggcgtgaga
    20281 gccagagtaa aatggcgcga agtgcattcg taccagtagc tgtagcccgg ctgattgatc
    20341 tcttttagcc gcttcagccg tgtgcgatac aacgtggcgc gctcaaatgc cgcatccagc
    20401 aaggcggaac gccccagtga cagtttcgcc acgtcataac aaagttccag ggtgtcatcg
    20461 agcagtaaaa atgcccgctg aatttgcgga ttagctaaca gctcacgcag gttaccgcga
    20521 taacctggct caccgagttg cagacgaaaa tcctgtgcac tctgggcaag acgatcagcg
    20581 cacttttgta actgctgggt gtcttttaat tcggtgcggt aggcgatggt gatgtctttt
    20641 gccaggtcga gcagttgtcg actggagagt gactgaccaa aatactggct ggcaatgtcc
    20701 ggtagctggt gggcttcgtc gaagatcatg acgtccgctt cagggatcaa ctcgccaaat
    20761 ccactctctt taaccaccat atccgccaga aagagatgat ggtttaccac caccacatcg
    20821 gcgtccatcg cttttttacg tgctttgacc acaaagcaat ctttatacat cgggcagtcg
    20881 ctgccaagac agttgtcgtt ggtgctggtg accagcggcc acgcctgtga atcttccgcc
    20941 acgctgacgc aggtgctgat atcaccatcg actgtttgat tagaccagga gcgcagcagg
    21001 atcacatcgc ttaagatttg taccggcaga tcgccccccg ccagcgcctg ctgttcgaga
    21061 cgttcgaggc agaggtagtt tgagcgccct ttcagcagcg ccacgttgcc cgtatatttc
    21121 aatgcctttg agactgttgg caaatcgcgg ctgtagagct gatcctgcaa cgcttttgag
    21181 ccggtcgaga taatgacttt ctttttcgcc cgcagcgcag gagccaggta agcgtaggtt
    21241 ttgcccgtac cggttcctgc ttccaccacc agcggctggc ctttttctat cgcctgggtg
    21301 acggctaccg ccatctgtcg ctgtggttct cgcggcttaa agcctggtat cgctttcgcc
    21361 agctgaccgt ctggtgcaaa atcgtccgtc acactacccc ctgttgattt gaacagggat
    21421 tatgtcagga tgagggcgct ttcgccagtt gaagtggtga cggcaacctc acattgtggc
    21481 agtctttgca gcacgaaaat ggaagtataa tgaggacaaa atgatgacta tcgttcgtat
    21541 cgatgctgaa gcccgctggt ctgatgtagt aatccacaac aacacgctct actacactgg
    21601 tgtaccggaa aacctcgacg ccgatgcctt tgagcaaacc gccaacacgc tggcacagat
    21661 tgacgccgtg ctggaaaaac agggcagcaa taaatcgagc attctggatg ccaccatttt
    21721 cctggccgat aaaaacgact tcgcggcgat gaataaagcg tgggatgcct gggttgtcgc
    21781 gggtcatgcg ccagtgcgct gcacggtaca agcgggtttg atgaacccga agtataaagt
    21841 tgaaattaag attgtggctg cggtgtaagc tttatcgaag caaaataagt cagacgataa
    21901 tttatcgata atactggtcg gttttacata aatcgaccag agaataagat attactgttg
    21961 atgcttctgc taattcatta tttatattta taatttcaat tttatctata aaactaacta
    22021 tataaataaa cataaccatg ccgttgtgtt cgccctttga ttagcaactc tggttttctt
    22081 tcttagctga acaatctggt tatagcgtgc ggactgaccg ttaattttct ccttcttact
    22141 ggataacaca ccatgagctg ctacctttaa aacacctgaa tgtcaacagg ttaactcgtg
    22201 ccatatcgtt ataatccctt tgatattaga tgcaaatta
//
LOCUS       DAESHI010000066        21995 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.066, whole genome shotgun sequence.
ACCESSION   DAESHI010000066 DAESHI010000000
VERSION     DAESHI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21995
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.066"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..986)
                     /gene="cmtA"
                     /locus_tag="KW011_002593"
     CDS             complement(<1..986)
                     /gene="cmtA"
                     /locus_tag="KW011_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBH7660897.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLVSFA"
     gene            complement(1014..1457)
                     /gene="cmtB"
                     /locus_tag="KW011_002594"
     CDS             complement(1014..1457)
                     /gene="cmtB"
                     /locus_tag="KW011_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HBH7660898.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            complement(1771..3762)
                     /gene="tkt"
                     /locus_tag="KW011_002595"
     CDS             complement(1771..3762)
                     /gene="tkt"
                     /locus_tag="KW011_002595"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBH7660899.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            4040..4798
                     /gene="loiP"
                     /locus_tag="KW011_002596"
                     /gene_synonym="yggG"
     CDS             4040..4798
                     /gene="loiP"
                     /locus_tag="KW011_002596"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HBH7660900.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(4941..5861)
                     /gene="speB"
                     /locus_tag="KW011_002597"
     CDS             complement(4941..5861)
                     /gene="speB"
                     /locus_tag="KW011_002597"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBH7660901.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5999..7975)
                     /gene="speA"
                     /locus_tag="KW011_002598"
     CDS             complement(5999..7975)
                     /gene="speA"
                     /locus_tag="KW011_002598"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HBH7660902.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7984..8115)
                     /gene="yqgB"
                     /locus_tag="KW011_002599"
     CDS             complement(7984..8115)
                     /gene="yqgB"
                     /locus_tag="KW011_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HBH7660903.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            8251..8466
                     /locus_tag="KW011_002600"
     CDS             8251..8466
                     /locus_tag="KW011_002600"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660904.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            8770..9924
                     /gene="metK"
                     /locus_tag="KW011_002601"
     CDS             8770..9924
                     /gene="metK"
                     /locus_tag="KW011_002601"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBH7660905.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            10360..11754
                     /gene="galP"
                     /locus_tag="KW011_002602"
     CDS             10360..11754
                     /gene="galP"
                     /locus_tag="KW011_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HBH7660906.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            11831..12328
                     /locus_tag="KW011_002603"
     CDS             11831..12328
                     /locus_tag="KW011_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HBH7660907.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            12423..13130
                     /gene="endA"
                     /locus_tag="KW011_002604"
     CDS             12423..13130
                     /gene="endA"
                     /locus_tag="KW011_002604"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HBH7660908.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            13210..13941
                     /gene="rsmE"
                     /locus_tag="KW011_002605"
     CDS             13210..13941
                     /gene="rsmE"
                     /locus_tag="KW011_002605"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBH7660909.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            13954..14904
                     /gene="gshB"
                     /locus_tag="KW011_002606"
     CDS             13954..14904
                     /gene="gshB"
                     /locus_tag="KW011_002606"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HBH7660910.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            15013..15576
                     /locus_tag="KW011_002607"
     CDS             15013..15576
                     /locus_tag="KW011_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HBH7660911.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            15576..15992
                     /gene="ruvX"
                     /locus_tag="KW011_002608"
     CDS             15576..15992
                     /gene="ruvX"
                     /locus_tag="KW011_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBH7660912.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(16167..17147)
                     /locus_tag="KW011_002609"
     CDS             complement(16167..17147)
                     /locus_tag="KW011_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBH7660913.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            17165..17869
                     /gene="yggS"
                     /locus_tag="KW011_002610"
     CDS             17165..17869
                     /gene="yggS"
                     /locus_tag="KW011_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HBH7660914.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17887..18453
                     /gene="yggT"
                     /locus_tag="KW011_002611"
     CDS             17887..18453
                     /gene="yggT"
                     /locus_tag="KW011_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HBH7660915.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            18450..18740
                     /locus_tag="KW011_002612"
     CDS             18450..18740
                     /locus_tag="KW011_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HBH7660916.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            18748..19341
                     /locus_tag="KW011_002613"
     CDS             18748..19341
                     /locus_tag="KW011_002613"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HBH7660917.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            19334..20470
                     /gene="hemW"
                     /locus_tag="KW011_002614"
     CDS             19334..20470
                     /gene="hemW"
                     /locus_tag="KW011_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HBH7660918.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            20781..21767
                     /locus_tag="KW011_002615"
     CDS             20781..21767
                     /locus_tag="KW011_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBH7660919.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSEGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQKQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
     gene            21812..>21995
                     /locus_tag="KW011_002616"
     CDS             21812..>21995
                     /locus_tag="KW011_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HBH7660920.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLG"
BASE COUNT         5441 a         5718 c         5597 g         5239 t
ORIGIN      
        1 gcaaaggaca ccagggtagc taccgtaaca ccggcaattg tcgccaggaa cgagcctttc
       61 ggcgtcagtg ccaggtaagc aaagatagac cccggacttg ggccagccac cagaccaccg
      121 tccagtaagt taaacaccca ggtgccagac ataccgcccg caatcatggc aataatggtc
      181 agcggcttca tcagcacata cgggaaatac agttcgtgga tcccaccgag gaagtgaata
      241 atcatcgccc cgggcgcaga acgtttactc atccctttac caaacaaggt aaacgccagc
      301 agcagcccta agccaggccc aggattggac gccaccataa agaagatgga tttaccgtta
      361 accgaagcct gttgcattcc cagcggataa tagacgccct gatcgatcgc attattgagg
      421 aaaagcactt tcgccggttc attgatgact gacagcagag gcagataacc cgcatgtacc
      481 agcgcctcaa tacactcttt gacgagagta ttggcaatta acaccgccgg gccgataact
      541 tcaaaaccca gcagacaaag gagcatcccc gcgataccta atgagaagtt attgataacc
      601 atctcaaaac cggcaggtat gcgtttttcc agtgccttat caacatattt tatgaccagc
      661 ccaccgagcg gccccataat cattgagcca aggaacatcg ggatctctgc gccgacgatc
      721 acaccaatag tacctattcc gcccatgacc gcgccgcgtt taccgccgac cagatgacca
      781 ccggtagaac caatcatcac gggcaataaa taggtaatca tcgggccgac aattttggcg
      841 aaatgttcgt taggcagcca accggtggga ataaataacg cagtaataaa accccaggca
      901 ataaaagcac caatattggg gatgaccatt gccgtcaaaa agcccccaaa agcctggacc
      961 tttgcacgag cagacttgtt ttccataata ttatcctgta gaggagagag taattatccg
     1021 cggctgatta tgtccgctaa ttgtttttct gatgctgctg ttaagagttg ttcgagtatt
     1081 tcttcttcgc acagtaattc acttaacgcc tgaatagcgc caatgtgcga atcggcatcg
     1141 gcagcagaaa ggccgatgag taattttatt ggttcgtcat tccccggaaa ataaacacct
     1201 tgttcaagta atgtcaaaga catcccggtt ttaagcgccc cacattccgg tcgcgcatga
     1261 ggcattgcca cgcctggtgc gagaatataa taagggccat tgttaatggt ggaatcttta
     1321 attgcctgaa tgtaattctc gctgatatag tttttatcca gcaatgatac catcgagaaa
     1381 tcgatagctt cctgccaatc ttttgctgaa tgtataactg agattgatga ttctggaaaa
     1441 tagtcactaa gccgcataaa ttatccttat tttattttta gggtaccgtc cggcggtaaa
     1501 aataaaatac tcgtgcggtt taatgaaagc aattgagcag ggaaaccggt gttttgaaaa
     1561 cagtgaatta ttaatttctt atataacatt gagttataga tataacaaca aatgtcaata
     1621 cgcatatcgt gatgcatatt acataattga tatttattgc atataaatat ttgtgatcta
     1681 caacacgctt tatctattgc ttgtccgcaa acggacatat caaggtaata aaaaaggtcg
     1741 ccgaagcgac ctttttaccc gaaatgctaa ttacagcagt tcttttgctt tcgcaacgac
     1801 gttgtcgacg gtgaagccga actcttcgaa cagcaactct gccggagctg actcaccgaa
     1861 ggtggtcata ccgatgatag cgccattcag gccaacatac ttgtaccagt agtcagcaat
     1921 acccgcttct accgcaacgc gtgcagtaac cgctttcggc agtacggatt cacggtaagc
     1981 cgcatcctgc ttgtcgaatg catcggtaga cggcatggaa accacgcgcg ccttcacgcc
     2041 ttcggcagtc agtttttcgt aggcggcaac agccagttca acttccgaac cggtcgcgat
     2101 gaagatcagt tccggctgac cagcacagtc tttcagcaca taaccaccgc gcgcgatgtt
     2161 tgccagttgc tcttcagtac gttcctgctg cgccagattc tgacgggaga ggataagcgc
     2221 ggtcggaccg tcctgacgct caacaccgta tttccacgcg accgcggatt caacctggtc
     2281 acacggacgc catgtagaca tgttcggggt cacgcgcaga gaagcgacct gctcaaccgg
     2341 ctggtgagtc gggccgtctt cgcccagacc gatggagtcg tgggtgtaaa ccatcacctg
     2401 acgctgtttc atcagcgcag ccatacgtac agcgttacgt gcgtattcga caaacatcag
     2461 gaaggtggag gtgtacggca ggaagccacc gtgcagggag ataccgttag caatcgcggt
     2521 cataccgaac tcgcgaacac cgtagtggat gtagttaccc gcagcatctt cgttgattgc
     2581 tttagaacca gaccacaggg tcaggttaga cggtgccagg tcagcggagc cgccgaggaa
     2641 ttccggcaac agcgggccga acgcttcaat agcattctga gacgctttac ggctggcgat
     2701 tttcgccgga ttagcctgca gtttagcgat aaactctttc gctttggcgt cgaagtcaga
     2761 cggcatttcg cctttcatac ggcgggtaaa ttcagccgct tcctgcggat aagctttcgc
     2821 gtaagccgca aacttctcat tccatgcaga ttctttcgcc tggcctgctt ctttcgcatc
     2881 ccactgcgca tagatttcag acgggatttc gaacggtgcg tatttccagc ccagctgttc
     2941 gcgggtcaga gcgatttctg catcacccag cggcgcaccg tgggagtcgt gagtaccggc
     3001 tttgttcggg gaaccgaaac cgatgatggt tttgcacatc agcagggacg gtttgtcagt
     3061 cactgcgcgc gcttcttcta ctgcgcgttt gatggatgct gcgtcatgac cgtcgatgtc
     3121 gcgaataacg tgccagccgt aagcttcgaa acgcattgcg gtgtcgtcgg tgaaccagcc
     3181 ttcaacgtga ccatcaatag agataccgtt gtcatcgtag aacgcaatca gtttacccag
     3241 cttcagcgta cccgccagag agcaaacttc gtgggagatg ccttccatca tgcagccgtc
     3301 gcccatgaag gcgtaggtgt agtggtcgac aatgtcgtgg cccggacggt taaactgcgc
     3361 cgccagcgtt ttttctgcaa tcgccatacc gactgcgttg gcaataccct gacccagcgg
     3421 accggtggtg gtttccacac cagcggtata acctacttcc gggtggcccg gagttttaga
     3481 gtgcagctga cggaagtttt tcagttcttc catcggcaga tcgtaaccgg tgaggtgcag
     3541 caggctgtag atcagcatgg agccgtggcc gttggacagc acgaagcggt cacggtcagc
     3601 ccaggacgga ttctgcgggt tgtgtttcag gaaatcacgc cacaggactt cggcaatgtc
     3661 agccataccc ataggggcac ccgggtgacc ggatttggct ttctgtactg cgtccatgct
     3721 cagcgcacga atagcattgg caagctcttt acgtgaggac attttgactc cagatcggat
     3781 gatgaagggc acgcccttaa cgacttgacg acagcgcgtt ttgggctacg ccggaaaatt
     3841 tgccaacaat ttaccgcaag ccgcgcgtca tgtacatgga acatcctttt gccgcttcag
     3901 aaatctctgg atcatgctcg catgttgcgc aatctactcg cccgtccgct gcgcttttcc
     3961 ttatactgag actgagcgtc gattcacctg caaacggcgc atttttagaa taatcctgac
     4021 cttgtgcgga agagaaaaca tgaaaattcg cgccttattg gtagcaatga gcgtggcaac
     4081 ggtactgact ggttgccaga atatggactc caacggactg ctctcatcag gagcggaagc
     4141 ttttcaggct tacagtttga gtgatgcgca ggtgaaaacc ctgagcgatc aggcatgtca
     4201 ggagatggac agcaaggcga cgattgcgcc agccaatagc gaatacgcta aacgtctgac
     4261 aactattgcc aatgcgctag gcaacaatat caacggtcag ccggtgaatt acaaagtgta
     4321 tatggcgaag gatgtgaacg cctttgcaat ggctaacggc tgtatccgcg tctatagcgg
     4381 gctgatggat atgatgacag ataacgaagt cgaagcggtg atcggtcacg agatggggca
     4441 cgtggcgtta ggccatgtga agaaagggat gcaggtggcg cttggtacaa atgccgtgcg
     4501 agtagctgcg gcctctgcgg gcgggattgt cggcagttta tctcaatcac aacttggtga
     4561 tctgggcgag aaattagtca attcgcaatt ctcccagcgc caggaagcag aagccgatga
     4621 ttattcttac gatcttctgc gccaacgcgg catcagcccg gcaggtcttg ccaccagctt
     4681 tgaaaaactg gcgaaactgg aagaaggccg ccaaagctca atgtttgacg accatccggc
     4741 atccgccgaa cgcgcccagc atattcgcga tcgcatgagc gcggatggga ttaagtaaag
     4801 cctggtggtg tcgaacgtag gtcagataag gcgttcacgc cgcatctgac atcaatggca
     4861 catttgttac cctgtgcgca ttgccggatg cgatgctgac tgcatcttat ccggcctacg
     4921 ggtggcatcg catctggtgc ttactcgccc tttttcgccg cttggatata cagcatctcc
     4981 agcgccagcg tcgccgcagc cagagcggtg atttccgact gatcgtatgc cggagccact
     5041 tccactacgt ccatcccaac aatgttgaga tctttcaggc cgcgtaccag tttaatagcg
     5101 cgatcggagg tcaggccgcc aatcactggc gtaccggtgc ctggtgcaaa agcaggatcc
     5161 aggcagtcga tatcaaaagt caggtaaacc ggcatatcac ccacaatctg tttcacttgg
     5221 gcgataacgt catccacgct gcgatcgttc acctggcagg cgtccagcac ggtaaagccg
     5281 ttgtctttat caaactcggt acgaatacca atctgcacgg aatgattcgg gtcgatcaga
     5341 ccttctttcg gcgcggtata gaacatagtg ccgtggtcaa attcacaacc gttcgcatag
     5401 gtatcggtgt gggcgtcaaa gtgtaccagc gccattttgc cgaaatgctt cgcatgagca
     5461 cgcagcagcg gcagcgtaac aaagtggtca ccaccgaagg tgagcatacg cttaccggca
     5521 gccagcagct tctcggcgtg cgcctgcagc ttttcgctca tctcacgggc atcgccaaag
     5581 gcatatacca gatcgccgca gtccacgacg ttcagacgct cacgcatgtc gaaattccac
     5641 gggaagcggt tgtgttccca ggccagattc gtcgaaacct gacggatcgc tgccggaccg
     5701 tggcgaccac ccgcacgacc agaagtggcc atatcgaacg gcacgccagt aatcacccag
     5761 tctgcatcgc tgtcatacgg ctggaagttc atcggcaggc gtaaaaaacc aaaggcattg
     5821 gaaaccagtg agttatcgta ttgatgacct aaggtgctca tggatggact cctattacaa
     5881 agtcgatata aaaaaaccct tccgcgtcgt taggcccgac gaggaagggt tggatttgtc
     5941 acaataaatt gtggcggatt atcaccgcta aattaagcgg attcaagtaa cacaggactt
     6001 actcatcttc aagataagta taaccgtaca aacctgcctc gaactcttca aggaactgtt
     6061 gttgcagttc agcatcaaga tcggttttct tcacttgatc gcggaactgg gttaacagcg
     6121 ttttcggatc gagctgtaca tattgcagca tgtccgccac ggtatcgcct tcgtcagaca
     6181 gttctacttc tacgctaccg tcagggaaca cgaacacgtc aaccgcttcg gtatcaccga
     6241 acaggttgtg catattgcca aggatctcct gatatgcgcc gaccataaag aaaccgagca
     6301 tcggcggatt ctctggatcg tactccggca ttggcattgt cgtggcaata ccatcaccat
     6361 caatatagtg gtcgatagca ccgtcagagt cacaggtaat atccagcagc acagcgcgac
     6421 gttccggcac ttgatccagc ccttccagcg gcagaaccgg gaacaactgg tcgatccccc
     6481 atgcgtccgg cattgactgg aacagcgaga aattgacgta cattttgtcc gccatacgtt
     6541 cctgcagttc gtcgattatc ggacgatgcg cacggttttg cggatccagt tgcttctgca
     6601 cttcatggca catgctcaga tagagttgtt ctgcccatgc acgttcttgc aggctaaagg
     6661 tgccggaaga gtagccgata tgaatgtcgt gcagatccat ctggctgtcg tgtaaccatt
     6721 cacgcaaaga acggcgagtt cccggttcgt gcatttcctg ccaggtttcc cacatgcttt
     6781 gcagcgcgcg cggcgcatct tctgcgggcg cggtcggcac cgtatattcg ttacgttcca
     6841 cgccgatgat attcgacacc agcacggtat ggtgcgcagt caccgcacgg cccgattcgg
     6901 tgattaccgt cggatgcggc agaccatttt cttcacacgc atcgccaatc gcccagatga
     6961 tgttgttggc gtattcattg aggccatagt ttacggagca gtcagactgc gaacgggtgc
     7021 cttcatagtc cacgcccaga ccgccgccga cgtcgaagca ctgaatattg acgcccagct
     7081 tgtgcagttc cacatagaaa cgcgcggatt cacgaacgcc cgtggcgata tcgcgaatat
     7141 tcgccatctg cgaaccgagg tggaagtgca gtagttgcag gctgtcgaga cgcccggctt
     7201 cacgcagggt ttcaaccagt tgcagtacct gagtcgcagc caggccgaac ttcgattttt
     7261 ccccgccgga ggactgccat ttacccgagc cctgcgaagc cagacgtgca cgcacgccca
     7321 gacgaggaac gacattcaga cgttctgctt catccagcac aatggcgatt tctgacatct
     7381 tctcaatgac cagatagacc ttgtgcccca tcttctcgcc aattaatgcc aggcggatat
     7441 attcgcggtc tttataaccg ttgcagacga tgacgctacg ggtcatgcca gcatgtgcca
     7501 gtactgccat caactcggct ttggaaccgg cttccagacc cagcggttcg cccgaatgaa
     7561 tcagggactc aatcacgcgg cggtgctggt taactttgat cggataaaca aggaagtaat
     7621 cgccgttata gccgtaggat tccctcgcac gtttgaacgc ggcgttaatg gaacgcaaac
     7681 ggtgctgcag gatctgtggg aaacagaaca gtgcaggcag acgctgaccc tgtgcttcac
     7741 gagttttcac taactgcgcg agatcgacgc gagcttccgg gacgtccggg tccgggcaca
     7801 cgctgatgtg gcccagctcg ttaacgtcat agtagttatt gccccaccag gcaatattgt
     7861 aagtacgcag catcttgctg gcttcctggg agctcattgc aacctcctgc atggagcgta
     7921 gtacaccgtg ttcgcccgct gacgaaggca aacccataga catgtcgtca gacatagcga
     7981 acctcaaatt attttattaa gtgtaaaaca gttaacgact atcgcagcct ggaactgcga
     8041 taacaaccca taagcacatg gattttccag cagtgaatgc tgacgctcca actgcgcaac
     8101 cggtttcttt ttcataacat tattaagcac ataaccgaac gtaagtgtga cagttcggcg
     8161 aaaccacgag aaaactcttg tttttacaag agcgcccttg ttcagtcctc agtaactgta
     8221 accagctctt gaatcctgag aagcgccgag atgggtataa catcggcagg tatgcaaagc
     8281 agagatgcag agtgcgggga acgaatcttc accagaacgg tgagacaggt taagcagcag
     8341 acaacggttc attatttcgt atcacctcca cggccgcctg ttaagacgaa cccacaagcc
     8401 aaaactctga tttcaacccg gctggaagtg gcaacacgaa agaaacgtcg tgtgcttttt
     8461 atttaagccg cgcgccgcgt tttatacccc acaatggcag aaaattgcaa aagataaata
     8521 cgcagaatgc cggcattgtc aggaaaaatt tccagccacg tttttaacag aatgagacac
     8581 gattcaaaaa aaagtggaaa tcgggtgaag aattgaccta aaatagccat ccagatgtta
     8641 atccatccat accgattaac actcagactg ccagtgtttt taacctgcag agtcgtggta
     8701 ggatccgcta ccacagaaaa tccacacaac agtttgagct aaccaaattc tctttaggtg
     8761 atattaaata tggcaaaaca cctttttacg tccgagtccg tctctgaagg gcatcctgac
     8821 aaaattgctg accaaatctc tgatgccgtt ttagacgcga tcctcgaaca ggatccgaaa
     8881 gcacgcgttg cttgcgaaac ctacgtaaaa accggcatgg ttttagttgg cggcgaaatc
     8941 accaccagcg cctgggtaga catcgaagag atcacccgta acaccgttcg cgaaattggc
     9001 tatgtgcatt ccgacatggg ctttgacgct aactcctgtg cggttctgag cgctatcggc
     9061 aaacagtctc ctgacatcaa ccagggcgtt gaccgtgccg atccgctgga acagggcgcg
     9121 ggtgaccagg gtctgatgtt tggctacgca actaatgaaa ccgacgtgct gatgccagca
     9181 cctatcacct atgcacaccg tctggtacag cgtcaggctg aagtgcgtaa aaacggcact
     9241 ctgccgtggc tgcgcccgga cgcgaaaagc caggtgactt tccagtatga cgacggcaaa
     9301 atcgttggta tcgatgctgt cgtgctttcc actcagcact ctgaagagat cgaccagaaa
     9361 tcgctgcaag aagcggtaat ggaagagatc atcaagccga ttctgcccgc tgaatggctg
     9421 acttctgcca ccaaattctt catcaacccg accggtcgtt ttgttatcgg tggcccgatg
     9481 ggtgactgcg gtctgactgg tcgtaaaatt atcgttgata cctacggcgg catggcacgt
     9541 cacggtggcg gagcattctc tggtaaagat ccatcaaaag tggaccgttc cgcagcctac
     9601 gcagcacgtt atgtcgcgaa aaacatcgtt gctgctggcc tggccgatcg ttgtgaaatt
     9661 caggtttcct acgcaatcgg cgtggctgaa ccgacctcca ttatggtaga aactttcggt
     9721 actgagaaag tgccttctga acaactgact ctgctggtac gtgagttctt cgacctgcgc
     9781 ccatacggtc tgattcagat gctggatctg ctgcacccga tctacaaaga aaccgcagca
     9841 tacggtcact ttggtcgtga acatttcccg tgggaaaaaa ccgacaaagc gcagctgctg
     9901 cgcgatgctg ccggtctgaa gtaatctttc ttcacctgct ttcaaaggcc agcctcgcgc
     9961 tggccttttt cttttacctc accgcggtgg ataggcattc acgccgcatc cggcaaaaag
    10021 ctgcccgcac aataacatca ttcttcctca acacgtttta tcgcaaatta tcatcactac
    10081 tgaaaccgat tacaccaata acaccagcca aatatttgta atattttcat attattattc
    10141 agttttcaca gttgttacat ttcttttcag taaagtctta attgcagata acagcgttta
    10201 atctatgatg atataactca attattttca tgcacttaaa ccataactaa gataaatgtt
    10261 agtgtaagcg attacactga tgtgatttgc ttcacatctt tttacgtcgt actcacctat
    10321 cttaattcac aataaaaaat aaccatattg gagggcatca tgcctgacgc taaaaaacag
    10381 gggcggtcaa acaaggcaat gacgtttttc gtctgcttcc ttgccgctct ggcgggatta
    10441 ctctttggcc tggatatcgg tgtaattgct ggcgcactgc cgtttattgc agatgaattc
    10501 cagattactt cgcacacgca agaatgggtc gtaagctcca tgatgttcgg tgcggcagtc
    10561 ggtgcggtgg gcagcggctg gctctccttt aaactcgggc gcaaaaagag cctgatgatc
    10621 ggcgcaattt tgtttgttgc cggttcgctg ttctctgcgg ctgcgccaaa cgttgaagta
    10681 ctgattcttt cccgcgttct actggggctg gcggtgggtg tggcctctta taccgcaccg
    10741 ctgtacctct ctgaaattgc gccggaaaaa attcgtggca gtatgatctc gatgtatcag
    10801 ttgatgatca ctatcgggat cctcggtgct tatctttctg ataccgcctt cagctacacc
    10861 ggtgcatggc gctggatgct gggtgtgatt atcatcccgg caattttgct gctgattggt
    10921 gtcttcttcc tgccagacag cccacgttgg tttgccgcca aacgccgttt tgttgatgcc
    10981 gaacgcgtgc tgctacgcct gcgtgacacc agcgcggaag cgaaacgcga actggatgaa
    11041 atccgtgaaa gtttgcaggt taaacagagt ggctgggcgc tgtttaaaga gaacagcaac
    11101 ttccgccgcg cggtgttcct tggcgtactg ttgcaggtaa tgcagcaatt caccgggatg
    11161 aacgtcatca tgtattacgc gccgaaaatc ttcgaactgg cgggttatac caacaccaca
    11221 gagcaaatgt gggggacagt gattgtcggc ctgaccaacg tacttgccac ctttatcgca
    11281 atcggccttg ttgaccgctg gggacgaaaa ccaacgctaa cgctgggctt cctggtgatg
    11341 gctgctggca tgggcgtact cggtacaatg atgcatatcg gtatccactc tccgtcggcg
    11401 cagtatttcg ccatcgccat gctgctgatg tttattgtcg gttttgccat gagtgccggt
    11461 ccgctgattt gggtactgtg ctccgaaatt cagccgctga aaggccgcga ttttggcatc
    11521 acctgctcca ctgccaccaa ctggattgcc aacatgatcg ttggcgcaac gttcctgacc
    11581 atgctcaaca cgctgggtaa cgccaacacc ttctgggtgt atgcggctct gaacgtactg
    11641 tttatcctgc tgacattgtg gctggtaccg gaaaccaaac acgtttcgct ggaacatatt
    11701 gaacgtaacc tgatgaaagg tcgtaaactg cgcgaaatcg gcgctcacga ttaatctcct
    11761 caggcttcct cccgtcgcgg aggaagccgc ctcttgcagt catcttttct tcgctctatc
    11821 ctctgccgct atgaaaacat cccgtctccc tatcgccatc caacaggccg ttatgcgcgg
    11881 cctgcgggaa aaactcgccc aggccaacct gaagctaggg cgtaactacc cggagccaaa
    11941 actctcttac acccagcgcg gaacctccgc cggaacagcc tggctggaaa gctatgaaat
    12001 ccgcctcaat cccgttttgc tgttggaaaa cagtgaagct tttattgaag aagtggtacc
    12061 gcacgaactg gcacatttgc tggtatggaa acatttcggc cgcgtagcgc cacatggcaa
    12121 agagtggaag tggatgatgg aaagcgtgct gggtgttccc gcccgtcgta cgcatcagtt
    12181 cgaactgcaa tccgtgcgtc gcaacacctt cccctaccgc tgcaagtgcc aggagcatca
    12241 gcttaccgta cgccgccata atcgcgtagt tcgtggcgag gccgtctatc gctgtgttca
    12301 ctgcggtgaa cagctggttg cgaaataacc atctgaacta tcaggaactt tcctgatctg
    12361 gctgattgca taccaaaaca actttcgcta cgttgctggc tcgttttaac acggagtaag
    12421 tgatgtaccg ttatttgtct attgctgcgg tggtactgag cgcagcattt tccggcccgg
    12481 cgttggccga aggtatcaat agtttttctc aggcgaaagc cgcggcggta aaagtccacg
    12541 ctgacgcgcc cggtacgttt tattgcggat gtaaaattaa ctggcagggc aaaaaaggcg
    12601 ttgttgatct gcaatcgtgc ggctatcagg tgcgcaaaaa tgaaaaccga gccagccgcg
    12661 tagagtggga acacgtcgtt cccgcctggc agttcggtca ccagcgccag tgctggcagg
    12721 acggtggacg taaaaactgc gctaaagatc cggtctatcg caagatggaa agcgatatgc
    12781 ataacctgca gccgtcagtc ggtgaggtga atggcgatcg cggcaacttt atgtacagcc
    12841 agtggaatgg cggcgaaggc cagtacggtc aatgcgccat gaaggtcgat ttcaaagaaa
    12901 aagctgccga accaccagcg cgtgcacgcg gtgccattgc gcgcacctac ttctatatgc
    12961 gcgaccaata caacctgaca ctctctcgcc agcaaacgca gctgttcaac gcatggaaca
    13021 agatgtatcc ggttaccgac tgggagtgcg agcgcgatga acgcatcgcg aaggtgcaag
    13081 gcaatcataa cccgtatgtg caacgcgctt gccaggcgcg aaagagctaa cctacactag
    13141 cgggattctt tttgttaacc cctaccccac gcgtacaacc gcgtggggag acgacgcgga
    13201 tttttaacta tgcgtatccc ccgcatttat catcctgaac cactgaccag ccattctcac
    13261 atcgcgcttt gcgaagatgc cgccaaccat atcgggcgcg tactgcgcat ggggccgggg
    13321 caggcgttgc aattgtttga cggtagcaac caggtctttg acgccgaaat taccagcgcc
    13381 agcaaaaaaa gcgtggaagt gaaggtgctg gaaggcaaga tcgacgatcg cgaatctccg
    13441 ctgcatattc acctcggtca ggtgatgtcg cgtggtgaaa aaatggaatt tacgatccag
    13501 aaatcgatcg aactcggtgt aagcctcatt acgccacttt tttctgagcg ctgcggcgtt
    13561 aaactggata gtgaacgtct gaacaagaag ctccagcagt ggcagaagat tgcaattgct
    13621 gcctgtgagc agtgtggtcg taaccgggtg ccggaaatcc gtccagcgat ggatctggaa
    13681 gcctggtgtg cagagcagga tgaaggactg aaactgaatc ttcacccgcg cgccagtaac
    13741 agcatcaata cgttgccgtt accggttgaa cgcgtccgcc tgctgattgg cccggaaggc
    13801 ggtttatcgg cagatgaaat tgccatgact gcccgctatc aatttactga tatcctgttg
    13861 ggacctcgcg ttttgcgtac agagacaact gcgctcaccg ccattaccgc gctacaagta
    13921 cgatttggcg atttgggcta acggagaaga ataatgatca agctcggcat cgtgatggac
    13981 cccatcgcaa acatcaacat caagaaagat tccagtttcg ctatgttgct ggaagcacag
    14041 cgtcgtggtt acgaacttca ctatatggag atgggcgatc tgtatctgat caatggcgaa
    14101 gcccgcgccc atacccgcac gctgaacgtg aagcagaact acgaagagtg gttttcgttc
    14161 gtcggtgaac aggatctgcc gctggccgat ctcgatgtga tcctgatgcg taaagacccg
    14221 ccgtttgata ccgagtttat ctacgcgacc tatattctgg aacgtgccga agagaaaggg
    14281 acgctgatcg ttaacaagcc gcagagcctg cgcgactgta acgagaaact gtttaccgcc
    14341 tggttctctg acttaacgcc agaaacgctg gttacgcgca ataaagcgca gctaaaagcg
    14401 ttctgggaga aacacagcga catcattctt aagccgctgg acggtatggg cggcgcgtcg
    14461 attttccgcg tgaaagaagg cgatccaaac ctcggcgtga ttgccgaaac cctgactgag
    14521 catggcactc gctactgcat ggcacaaaat tacctgccag ccattaaaga tggcgacaaa
    14581 cgcgtgctgg tggtggatgg cgagccggta ccgtactgcc tggcgcgtat tccgcagggg
    14641 ggcgaaaccc gtggcaatct ggctgccggt ggtcgcggtg aacctcgtcc gctgacggaa
    14701 agtgactgga aaatcgcccg tcagatcggg ccgacgctga aagaaaaagg gctgattttt
    14761 gttggtctgg atatcatcgg cgaccgtctg actgaaatta acgtcaccag cccaacctgt
    14821 attcgtgaga ttgaagcaga gtttccggtg tcgatcaccg gaatgttaat ggatgccatc
    14881 gaagcacgtt tacagcagca gtaacccacc ttagcgagaa ggatctcgtt gagactctga
    14941 gtgacagcgc ccttctttcc acgcatactg ggcgctgttg cttttttgaa ccaggaaaca
    15001 gaacctctga caatgaattt acagcatcac tttcttattg ccatgcctgc tctccaggat
    15061 ccgattttcc gtcgttccgt ggtctacatt tgcgaacata ataccaatgg tgcaatgggg
    15121 atcatcgtca acaagccgct ggaaaatctc aaaattgaag ggattctgga aaagctgaag
    15181 atcacgccgg agccgcgtga tgaatcaatc cgtctggata aaccggttat gctcggcggt
    15241 ccgctggctg aagatcgcgg gtttattttg catactccgc cctccaattt tgcttccagc
    15301 attcgcattt cagacaacac agtgatgacc acttcccgcg atgtgctgga aacgctcggc
    15361 accgataaac aaccgtctga cgtattggtg gctctaggtt atgcctcctg ggaaaaaggt
    15421 caactggaac aagaaattct cgataacgcg tggctaacgg ccccggcgga tctgaatatt
    15481 ctgttcaaaa cgccgattgc cgaccgctgg cgcgaggcgg caaaactgat tggtgtggat
    15541 attctcacca tgcctggtgt ggcagggcac gcctgatgag tggaacctta ctcgccttcg
    15601 acttcggcac caaaagcatt ggcgtagcgg tcggccaacg cattaccggc actgctcgcc
    15661 ctttgcctgc aattaaagca caggacggta cgccggactg gaacctcatc gaacgtttac
    15721 tgaaagagtg gcagccggac gaaatcatcg tcggtttgcc gctgaatatg gacggcaccg
    15781 agcaaccatt gactgccaga gcgcgtaaat ttgccaaccg tattcatggt cgtttcggtg
    15841 ttgaagtaaa gctccatgac gaacgtctta gcaccgtgga agcccgttcc ggtctgtttg
    15901 aacagggcgg ctatcgggcg ctcaacaaag gcaaagtcga ctctgcctct gcggttatta
    15961 ttctcgaaag ctatttcgag cagggatatt aaggcgattt aaaagcctgg cggatagtcg
    16021 ttaccgccat cttgccggat gcggcgtgaa cgcctatccg gcctacatgt ggcagcgatt
    16081 gtaggtctga taagacgcgc aaacgtctca tcagacgttt attgcctttg taggcccgat
    16141 aagcttgcac atcgggcatg gcaacgtcac aaacgccctt cccccactcg ctgctgaaaa
    16201 ctctgctgaa acgttatcat ccccacctgc tgcccggttt gaataacatg cggtaactgg
    16261 tgggttttcc cttcgcgaat caaattcccc accgcgggag tgttaatcaa taattcaaac
    16321 agcgccacgc gtccttcctg tttatccact tccagctttt gtgacaacac ggcccgtaaa
    16381 ctacctgcca gttgattacg tacgggatct ttttcctgcg ccggaaatga atccaccagt
    16441 cgctcaactg cctgcgcggc accacgcgta tgtaatgttg ccagcaccaa atgcccggtt
    16501 tctgccgccg tcagtgccag acggattgtc tcgctgtcac gcagctctcc gagcaaaatc
    16561 acatcaggat cttcccgcaa tgcggcccgc aatcccgatg cgaacgtcat acagtgcaaa
    16621 ccaatttccc gcttctggat caaacatcgc tggctggcat agatatattc aacaggatct
    16681 tccagcgtca gaatatgcgc atcggcatgt tgattgagat agccaaccat cgccgccagc
    16741 gtggtagatt tgctgctccc cgtcgccccc gtcaccagaa tcaggccatt ctcactcttg
    16801 agtaattccg gcaataccgt tggtgcgcca agctgttcga gctgcgggca gtgcgaaggt
    16861 aacagccgta acgccagcga aatgccatgc cgttgcgcaa atgcgctgcc acgcaatcgc
    16921 tggttttccg ccagcgacac ggcaaaatcc agctggccat tttccagcaa tattgcccgc
    16981 tgatcgtcat ccagccactc ccgcagtagc tcttcgacgt ccggcgcctc aaacggcgca
    17041 gcttccatta gcccgcgaat gcgccatcgt gcgggccagg cgctgcacag gtgtagatcc
    17101 gagacgttat gctttacact aagggccaca atttcttcca tattcatact aagatcctcg
    17161 gaaaatgaac gatattgcgc ataacctggc acaggtccgg gacaaaatct cagcggctgc
    17221 aacgcgttgc ggccgttctc cagaagaaat tacgctgctt gcagtcagta aaacaaaacc
    17281 tgcgagcgcc atcgcagaag ccattgatgc cgggcagcgt caatttggtg aaaactacgt
    17341 tcaggaaggg gtagataaaa ttcgccactt tcaggaactg ggcgtaacag gattagaatg
    17401 gcattttatt ggcccgttgc agtctaataa aagccgcctg gtggcagagc atttcgactg
    17461 gtgtcatacc atcgaccgtt tgcgcattgc tacccgcctc aacgatcagc gcccggcaga
    17521 acttccccct cttaacgtac tgattcaaat taatattagt gatgaaaaca gtaagtccgg
    17581 gattcaactg gcagaactgg acgagctggc agcggcggtc gctgaactac cgcgtttacg
    17641 tctgcgcggg ctgatggcaa tccctgcgcc tgagtcagaa tatgtaaggc agtttgaagt
    17701 tgcacgccaa atggctgtag catttgccgg actgaaaacg cgctacccgc atatcgacac
    17761 gctctctctg ggaatgtcgg acgatatgga agccgccatt gcggcaggta gcacgatggt
    17821 tcgtatcggc actgcaattt ttggtgcgcg tgattactct aaaaaataag gaattaaagg
    17881 aacgccatga atacgttgac tttcctgctt tcaacggtca ttgagctgta taccatggtg
    17941 ctgttattac gcatctggat gcagtgggct cattgtgatt tttacaaccc cttctcacag
    18001 tttgtagtga aggtaacgca gccaattatc gggccactgc gccgcgttat tccggcaatg
    18061 ggccccattg acagcgcctc gctgctggtt gcctatattc tcagttttat caaagccatc
    18121 gtgctgttta aagtggtgac cttcctgcca atcatctgga ttgctggttt actgattctg
    18181 ctgaaaacca tcggcctgct gattttctgg gtcctgctgg tgatggcgat tatgagctgg
    18241 gtaagccagg gtcgtagtcc gattgaatac gtgctgattc agctggccga tccgctgctg
    18301 cgcccgattc gccgcctgct accggcaatg ggtgggattg atttctcgcc gatgatcctc
    18361 gttctgctgc tgtatgtcat caatatgggt gtcgcagaag tattacaggc aaccggaaac
    18421 atgctgctgc cggggctgtg gatggcgtta tgagtgccgt aacagttaat gatgacggtc
    18481 tggttttacg gctctatatt cagccgaaag ccagccgtga ttctattgtc ggtttacatg
    18541 gcgacgaagt taaagtcgcc attaccgcgc cgccggttga cggccagggc aacagtcatc
    18601 tggtgaagtt tctcggtaag caattccggg ttgccaaaag ccaggtggtg attgaaaaag
    18661 gcgaacttgg ccgccacaaa caaattaaaa tcattaatcc gcaacaaatc ccgccagaaa
    18721 tcgcggcgtt aattaattag gtatcctatg caaaaagttg tcctcgcaac cggcaatgcc
    18781 ggtaaagtgc gtgagctggc gtcgctgctt agcgacttcg gtcttgatat cgtggcccaa
    18841 acagacctcg gcgtagattc cgctgaagaa accggcctga cctttatcga aaacgcgatt
    18901 ctgaaagcgc gctatgcggc aaaagtgacc ggtttaccgg caattgccga cgactctggt
    18961 ctggcggtag atgcgctggg tggcgcgccg ggtatttact ccgcgcgtta ttccggtgaa
    19021 gacgcgaccg atcaaaagaa tctgcaaaaa ctgttggaaa cactgaaaga cgtaccggac
    19081 gaccaacgtc aggcgcgttt ccactgcgtg ctggtatatc tgcgtcacgc ggaagatccg
    19141 actccgctgg tatgccacgg cagctggccg ggcgtgatta ctcgcgaacc agcgggcact
    19201 ggtggctttg gttatgatcc aatcttcttc gtaccttccg aagggaaaac cgccgccgaa
    19261 ctgacccgcg aagaaaagag cgccatttcc caccgtggtc aggcgttgaa actgctgctg
    19321 gacgctttac gtaatggtta aattaccgcc gctgagtctc tacattcaca tcccgtggtg
    19381 cgtgcagaaa tgcccgtact gcgatttcaa ctctcacgcg ttgaaaggag aagtgccgca
    19441 cgacgattat gttcagcatc tgctttgcga tctggacaat gatgtggctt acgctcaggg
    19501 ccgtgaagta aagacaatct ttattggcgg tggtacgccg agcctgcttt ccggcccggc
    19561 gatgcaaacg ctgctggacg gcgtgcgtgc gcgtttgccg ctggcagcgg atgcagaaat
    19621 tactatggaa gcgaaccctg gaacggtaga agccgatcgc tttgtcgatt atcagcgtgc
    19681 tggtgtgaac cgcatctcta ttggtgtgca gagttttagc gaagaaaagc tgaaacgact
    19741 tgggcgtatt catggcccgc aagaagcgaa acgcgcggca aagctggcga gcgggctggg
    19801 gctgcgtagc tttaacctcg atttgatgca tggactaccg gatcaatcac tggaagaggc
    19861 gcttggcgat ctacgccagg ccattgaact gaatccgccg catctttcct ggtatcaact
    19921 gaccatcgaa cccaatacgc tgtttggttc gcgaccaccg gtgctgccgg acgatgacgc
    19981 gctgtgggat attttcgaac aggggcatca gttattaacc gcagcgggtt atcagcaata
    20041 tgaaacttcc gcttacgcca aacccggtta tcagtgccag cacaatctca actactggcg
    20101 atttggtgac tacatcggta ttggctgcgg cgcgcacggc aaagtcacct tcccggatgg
    20161 gcgcattctg cgtaccacta aaacgcgtca tcctcgtggt tttatgcaag gaaggtatct
    20221 ggaaagccag cgtgatgtcg aagccgcaga taagccgttt gagttcttta tgaatcgctt
    20281 ccgtctgctg gaggccgcgc cgcgcgtgga gtttattgcg tataccgggc tttgcgaaga
    20341 tgtgattcgc ccacagttag acgaggcgat tgcccagggt tatctcaccg aatgtgcgga
    20401 ttactggcag ataaccgagc acggtaaatt attcttaaac tcacttcttg aattattcct
    20461 cgctgagtaa tcattaatgc gcctgcgggc gcattaatca cttcttttcc ccccgttaat
    20521 taacttcggc aaaatatatt tcttactgaa taaacatcga tgatatttat tagatatatc
    20581 tagtgaatat ttactcatca tttattgatt ataaacatgt acaacctggc gcacaaattt
    20641 aatatttaat ttctgacttt atttcaagtt gtgataaaca tagacaagtt gtcagacagg
    20701 ttaaccaatt tgtcgcacaa agcactgata agcacaatta gatatcaacc aatttccatc
    20761 cttcaacagg gagttttttt atgaaaatga ggtcatttac ccgctcatta gtctgcgcat
    20821 ccttgctggc tctggttagc actggcgtaa acgccgcaga aaaagtgacg ttaaagttag
    20881 cacacaacct ggaaagaagt catgttgtac atcagagttt tgaagagctg gcgaaagagg
    20941 taaaacagct gtcagaaggt aatatggtga tccgtattta tcccagcagc cagatgggta
    21001 atgcgcgaga aactatggaa ttgttgcaaa atggcgcact ggatatgacc aaaggttccg
    21061 ccagtgatct ggagtctttt gataatattt atgctatcta taatttgccg ttcttattta
    21121 aagatcaggc tcacttcaac aaagttgttt ttggtgaagt tggtaaagaa attatggact
    21181 caacaaaaga caaaggcttt tttgctttat cagcttatgt agcgggtaca cgcagtttct
    21241 atgcgaaaaa accgattact aagccagaag atcttaaagg gctgaaaatt cgtgttcagc
    21301 caagtccgac caccatcaaa atgattgaac tgatgggtgg ttcacccact ccaatttcat
    21361 ttggcgaagt ttatactgcg atgcagcaag gtgtagtaga tggcgcagaa aataacgtgc
    21421 cttcctgggt gcagactcga catattgaaa ttgccaaagt tttttctgaa gatgaacacg
    21481 cctcaattcc tgactttttg gttatctcca ccaaaacatg gaataaatta accccagaac
    21541 agaaacaaat cctcgaaaca gcagcgaaga aatccgaagc ttatcaacaa aaattgtggg
    21601 aaaaaattga cgctgacaca cgtgctcagg cgaaagctat ggggggtgaa attgtaaaag
    21661 tggataaagc accattccgt gccgcagttc agcctctgtt tgacgacttc aaaaaagatc
    21721 caaaacaagc tgctttgtta gagaagttcg acaacgctgc tcaataatat tccgggccag
    21781 tctctggccc tgattttcca agatgcttac aatgatattt aaccgcctga aacttgcggt
    21841 agaccgcgtc attgccgcgt tttctgtcgc cgtcatgctt gctctggttg tatgcgtagt
    21901 ctggcaagta tttagccgct acgtacttaa ccagccaagt acattaaccg atgagctggc
    21961 acgatttttg atgatctggg tagggttatt aggtg
//
LOCUS       DAESHI010000067        21972 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.067, whole genome shotgun sequence.
ACCESSION   DAESHI010000067 DAESHI010000000
VERSION     DAESHI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21972
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.067"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(121..330)
                     /locus_tag="KW011_002617"
                     /pseudo
     CDS             complement(121..330)
                     /locus_tag="KW011_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="KW011_002618"
     CDS             complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="KW011_002618"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HBH7660921.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="KW011_002619"
     CDS             complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="KW011_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HBH7660922.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="KW011_002620"
     CDS             complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="KW011_002620"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HBH7660923.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="KW011_002621"
     CDS             complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="KW011_002621"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBH7660924.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3522..5123)
                     /gene="tap"
                     /locus_tag="KW011_002622"
     CDS             complement(3522..5123)
                     /gene="tap"
                     /locus_tag="KW011_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HBH7660925.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5169..6830)
                     /gene="tar"
                     /locus_tag="KW011_002623"
     CDS             complement(5169..6830)
                     /gene="tar"
                     /locus_tag="KW011_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HBH7660926.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="KW011_002624"
     CDS             complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="KW011_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBH7660927.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="KW011_002625"
     CDS             complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="KW011_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBH7660928.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9468..10394)
                     /gene="motB"
                     /locus_tag="KW011_002626"
     CDS             complement(9468..10394)
                     /gene="motB"
                     /locus_tag="KW011_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HBH7660929.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10391..11278)
                     /gene="motA"
                     /locus_tag="KW011_002627"
     CDS             complement(10391..11278)
                     /gene="motA"
                     /locus_tag="KW011_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HBH7660930.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="KW011_002628"
     CDS             complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="KW011_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HBH7660931.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="KW011_002629"
     CDS             complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="KW011_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HBH7660932.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            13115..13543
                     /gene="uspC"
                     /locus_tag="KW011_002630"
     CDS             13115..13543
                     /gene="uspC"
                     /locus_tag="KW011_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HBH7660933.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(13550..14974)
                     /gene="otsA"
                     /locus_tag="KW011_002631"
     CDS             complement(13550..14974)
                     /gene="otsA"
                     /locus_tag="KW011_002631"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HBH7660934.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(14949..15749)
                     /gene="otsB"
                     /locus_tag="KW011_002632"
     CDS             complement(14949..15749)
                     /gene="otsB"
                     /locus_tag="KW011_002632"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HBH7660935.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(15916..16902)
                     /gene="araH"
                     /locus_tag="KW011_002633"
     CDS             complement(15916..16902)
                     /gene="araH"
                     /locus_tag="KW011_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HBH7660936.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(16917..18431)
                     /gene="araG"
                     /locus_tag="KW011_002634"
     CDS             complement(16917..18431)
                     /gene="araG"
                     /locus_tag="KW011_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HBH7660937.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(18501..19490)
                     /gene="araF"
                     /locus_tag="KW011_002635"
     CDS             complement(18501..19490)
                     /gene="araF"
                     /locus_tag="KW011_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HBH7660938.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            20287..20790
                     /locus_tag="KW011_002636"
     CDS             20287..20790
                     /locus_tag="KW011_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HBH7660939.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(20869..21120)
                     /locus_tag="KW011_002637"
     CDS             complement(20869..21120)
                     /locus_tag="KW011_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HBH7660940.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(21235..21321)
                     /gene="azuC"
                     /locus_tag="KW011_002638"
     CDS             complement(21235..21321)
                     /gene="azuC"
                     /locus_tag="KW011_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HBH7660941.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            21584..21907
                     /locus_tag="KW011_002639"
     CDS             21584..21907
                     /locus_tag="KW011_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HBH7660942.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT         5370 a         5840 c         5176 g         5586 t
ORIGIN      
        1 gagtcggcga attacatagg ggctcgcagc gttacggctg caataaagcc ggatataaag
       61 ctgcaaccta cccgtcatgt caaaacaatg gatgccttgc aaacgggatg cgttcatata
      121 aatactgtga tcaaaatgca gcccagcgga gagcatgccc gacaatcgac gggccagcga
      181 cacaccgcca aaccagataa cacacacgcc cactaacaaa atcagcaacg aggtcagttc
      241 acgggaacgc gggatttgcc cctcttcccg cgctttttct agtcggtgag gtgtgggggc
      301 ttctgttttg tcgtcgctct cgtcagacac gtcgccaatc ctggaaagag tattaagctt
      361 aaatgatgcc agagcgcaaa gcgttcaatg gtttgagtaa ggggcaaaac aggcgggatt
      421 tagggctttt gctgccacac atcaagcata gtgtgcgttt gtcggatgcg gcgtcatcgc
      481 cttatcagac cgcctgatat gacgtggtca cgccacatca ggcaatacaa atcaaaatcc
      541 aagactatcc aacaaatcgt ccacctgatc ctgactggct accacgccga ctttgctggt
      601 atcgacctga ggtccattaa gcaaactctg gttttcacgt tttggacgcg actcctgttc
      661 cgggatgttt tccaacagca ccatcagcaa ctggcgttcg atctcctgaa tgacatccat
      721 catccgctta atgaccagcc cggtgagatc ctgaaaatcc tgcgccatca tgatttccag
      781 cagttgcgcg ttagtaaagc tggtatgcgc gggtacatct gccagaaatt gtcgtgtatc
      841 tgttaccagt tcacgggcat cggcaaggtc aatcggatcg gcaaaccagt catcccaacg
      901 ttgggttaac gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac
      961 actgttcagc gcccgctccg cagcctgggc ggtcatctgc acaacatagt acaaacgatc
     1021 gcgcgcatcg gggatggctt ccgccgcttc ggcaatggcc tgatccagcc ccagttcccg
     1081 caaactgtcg cgcagcatac gcgtcaggct gccgatgcgc gcaatgatat cgccagctga
     1141 atgctcgtca gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt
     1201 tctcaaagat tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat
     1261 agccactggc ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct tctgcagtca
     1321 ccattaacac tggcaatgcc gacatcgcgc catccgcacg aattgttttc agcaattcca
     1381 ggccatccat attgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca
     1441 acttattgag agcgtcgacg ccatcttccg cttcctcaac attattgaat cccagctctt
     1501 tcagcaggtt acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa
     1561 gttctttatc cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat
     1621 ttttgccaac atttgctggc ttacctggct aagatcgacc acttcgcaga caccacccat
     1681 attgatggcc tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gcgcaagggt
     1741 ccatgccccc gcctgacgca tcgccaacat tcccgccgcg ccgtcgttgc ccataccggt
     1801 caggatcacc ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc
     1861 taccgaaggc cgatgacggt taaccgccgg gccgtcgtga attttgattt gataatttgc
     1921 gccactacgc gccagttcca tatgccgatc gcccggcgca atataggcat gccccggcaa
     1981 gacacgttct ccgtcttcgg cttctttaac cccgatctgg caaagcttat taagtctgtc
     2041 ggcaaaagag cgggtgaaac cgggcggcat atgttgggta attaacaatg ccgggctgga
     2101 aagcggcaac ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat
     2161 cgcaatcagt ttttcagaac tcaacaacgg acccgccttc agcgttgtcg gtgctgacaa
     2221 tggcttatgt gctgcaaggc tcgcctttgc tgccgtacgc accttttcag caatcatttc
     2281 gctatacgcc agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctattgc
     2341 ccccagctcc agcgcgcgca gcgtgacttc tgatcctttg ccggtcaagg aagaaaccat
     2401 cacaacgggc attggacgca aacgcattaa tttttcgagg aaatccagtc cgtccatccg
     2461 cggcatttca acatccagcg tcagcacatc gggattgaat ttcttaatca agtcacgcgc
     2521 gaccagcgga tcaggcgcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc
     2581 tgtcatgatc tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat
     2641 cgttaatcct tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg
     2701 tggctaaagt tttcagagtg acccgcaaac agcaatccgt cgggtttaag gagcggaaca
     2761 aagcggcgca aaatctcctg ctgggtattt tgatcgaagt agatcatgac gttacgacag
     2821 aagatcgcat caaacggccc cggcacggtg tactgtttcg ccagtagatt cagcggggca
     2881 aaatcaacat agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc
     2941 cctcgcatga aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga
     3001 taaataccgc ttctggcttt ttccagcact tcggtgtcga tatcactggc aaacactttc
     3061 cagcgtccgg gcgcggtgcc caatgtgtca gccagcgtca tcgcaatgct gtacggctct
     3121 tcgccggtcg aagccgccgc gctccatacg cgatactcgc cagaacggcg acgtgcgtga
     3181 tccgcgagca gagggaaatg gtgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc
     3241 gaattgataa acgcctgcca ctcaccgctg tgctgattag attccagcaa gttcagataa
     3301 tgaccgaaat ccgtcagtcc cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg
     3361 tcgcgtttat ggtcagccag aacgatcccg gctcgttgat agatcaattg acttatccgc
     3421 cgaaaatgcg cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc
     3481 ccacagggca gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca
     3541 atttgtaact gcgccgactc atgtcgtgct acttcatgtt cttcaagggt aaataccgcc
     3601 acacgcgacg aaagatggtc ggcctgattc gccagttgtt ccgttgccac cgccgcttct
     3661 tctaccagcg aggcgttctg ctgagtcacc tgatccatct ggctgacagc ctgtgcaacc
     3721 tgctcaatcc cccgctgttg ttcttccgac gcagaggcaa tttctcccat aatgtcgttc
     3781 acgcgggtca ccgaactgac aatatcgatc atggtcgcgg cggcgttatt caccagtttc
     3841 gaaccctgct ggacacgatt gactgactct tcgatcagcc ctttgatctc ttttgctgcc
     3901 tgcgcgctac ggctggcaag attgcgcact tcacctgcca ctaccgcaaa accacgcccc
     3961 tgctctccgg cgcgagccgc ttccactgcc gcattcaggg ccagaatatt ggtctggaaa
     4021 gcaattccgt cgataacgct gataatgtcg ccaatttttt gcgagctggt ggcgatctcc
     4081 tgcatggtgt gagtcatggt actgacctga acaccgcccg cctgcgccgt tgtcgcggca
     4141 ttttttgcca gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt
     4201 aattgctcca tactggcggc cgtttgtgcc agcgatgccg cctgctgttc ggtacgactt
     4261 gagagatcgt tattgcctgc gacaatctcc gcgataccaa tgtgcatctc ctggcttccc
     4321 ttacgcacat cacttaccgt cccacgcaaa gcctgctgca tggtcttcag actggcaaaa
     4381 atggcggtga tctcattacg accatatacc gcaatcggac gcgccagatt acccgcagca
     4441 atgctgtcaa aatggctacc gataatggcc agtggttgaa caatcatctt gcgcgtccac
     4501 cacagcgcac tgctgatgta gatcgccgca acaataatca tgctgataaa caccagtgtc
     4561 gaaatctgat agttacgctg gctttgcgca ctggcggctt ccagcacatg gttgatctcc
     4621 agctgccagg cctcaaagtt aacgtcaaac gcattctgtg atccctgcac cggcgcagtg
     4681 aggaaatccg aaagttgatt actttcaagc caggtcgcct gatgttcgag atcgttgtgc
     4741 cagcgggcaa aacttttctc cgtctctttt tgcaatgccc tgacgtgctc gttgcccgca
     4801 gtcatcgcca taaaactttt aaacagcgtg gtggattgcg tcagactggc gcgcgccgtc
     4861 gtcatcaacg ttttaatgtc atccgccgga tagctaagtg ccgtcagagt gcccgctttg
     4921 ttcagcgcgg tactggcctg taacattacc gcccgagttt gcgctaatgc cgcacgttgc
     4981 tgattgctct gctcgacctg attcagtcgt tgcaaatcgt cgcgaaatgc ccaaaaagac
     5041 atgccgttac tgccaatctg caagatcccg cagagaatca aaattaaaaa cagcgtggtc
     5101 gaaattcgaa tacgattaaa catcaacgct ccccatcagg cggcaatgac cgcgttagta
     5161 aatactcgtc aaaatgtttc ccagtttgga tcttgttcag caattcgcag tcgtggctgt
     5221 gccggtggtt gctcactggc aggacgggat ggtgtttgcg gtttattggt gagtgggctg
     5281 gctgccagac ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc ttccagcgca
     5341 gcggcggcgg cagctgattc ctgcaccagc gatgcgttct gttgcgtgac gcgatccatt
     5401 tccgaaaccg ccaatgcgac ttgatcgatg ccacggctct gttcatccga tgccgatgca
     5461 atctcgccca taatgtcagt cacgcgagtg acagcattga cgatattgtt cattgtttcc
     5521 ccggcgcttt cgaccagcac cgaaccggta tcaacgcgtg cgacggagtc ttcaatgagg
     5581 gctttgatct cttttgccgc ctgggcgctg cgactggcaa gattacgcac ttcacccgcc
     5641 accacggcaa aaccacggcc ctgttcaccc gcacgcgcgg cttcaaccgc ggcattcagc
     5701 gcgaggatat tagtctggaa ggcaataccg tcgataacgc tgataatgtc ggcaattttc
     5761 ttcgaactat cggcgatctc atgcatcgtt ttcactacgc catccaccac tttgccgccg
     5821 tgctgggcgg tgtcggaggc actttgcgcc agttgcgagg cctggcgggc gttatcggcg
     5881 ttttgcttca ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc cagcgcggat
     5941 gcctgctgtt cagtacggga ggaaagatcg gtgttgcccg ccgcaatttc acgggtaccg
     6001 gcatagatgg catctgaacc ttcgcggaca tgagtgacgg tgtcagtcaa agagcgttgc
     6061 atatgtgaaa cgctctgcgc caggtcgccc atttcactgc gcccgtcaat ggtcagggta
     6121 ttcgctaggt taccaccggc gatttcgcga atgtgagcaa taatttttgc cagcggagta
     6181 agcaacatac ggcgaatgcc gtaccacgcc accagcagaa tcaataccac caccagcgcg
     6241 ataaccgcca gttgccactg ggcaaatcgg taatcatctg cgttgtcagt gacgatatcg
     6301 cgatacagtt tttcactgct gagggcgtac tgagcaaacg cttcgcccat tgcattttgc
     6361 attccctggg ttggctgagc gaaataagct ccagtattgc cataatctag ataatcaatc
     6421 agttcagtta acgctgtgta atagttttta tatttttcat caatattacg actggtagcg
     6481 accatttcag gtaacggtgc catgcttttg aattttttat aatgcgtcgc tgcctgcgcc
     6541 aatgttttcc tggcgctatc gagcaattca actttggcgt tactttgttg attggaggaa
     6601 tccatcatca tccgcaccgc tgaacgactc aggttaatgc gcgtttgcag cattaaatcc
     6661 caggttgacg tcagctcgcc ctgctgttcc cgcaattgat tggaaaccac aaagctcttc
     6721 tggctatggt gaagggaaga aaaaaacaga ctgccggaaa taagctgtaa cagtgcgaat
     6781 acccccagca ccattaccaa cagcgtgact acgcggatac ggttaatcat aaggcacctt
     6841 cctgaaaaca agttgatctc gttatcggca agaagggggg aaactttatt gctgatgcca
     6901 cccgccgcga aattgaaata aaaaacccga tgcgcagatc atcgggttca tttcaattga
     6961 ggaaatcggg agaattacgc cacttctgac gccgcgctat ctaacagcgc catctcttcg
     7021 ctgttcagca gtttttcgat gttcaccaga atcaacatac ggtcgcccag tgcgcccagt
     7081 ccagtgagat attctgttga aagcgtcacg gcaaactctg gtgccggacg aatttgctcc
     7141 gccgtcaatg aaagcacgtc tgaaacgccg tcaaccacga tgccgaccac ccgctgtccg
     7201 agattcagga cgataactac cgtgttgtcg ttatagtcca catccacctg gctgaactta
     7261 attcgtaagt caacaatcgg cacaataacg ccgcgcagat tcgtgacgcc tttgataaac
     7321 gctggcgtgt tcgcaatccg tgttacctga tcgtagccac ggatctcctg cactttcagg
     7381 atatcaatac cgtactcttc atcaccaagg gtaaatacca gaaattcctg gcctgacggc
     7441 tcgctggcca gctttgttac attcgtcata ccggtcatat tgttaccttt ttactcattc
     7501 aggcggcggt gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg gagacatcaa
     7561 caatcagtgc cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg gggactttgc
     7621 gatagttact ttcaaggttt ttaaccacaa cctggtgttg accaattaat tgatccacca
     7681 gcaaggcgta gcggcgaccg ccactttgta agatcacaac aattccctgg gtggcttcgg
     7741 ttttcgcgcc cgcgacgttg aacactttcc acagttcgac gatgggcaga tattcacccc
     7801 gcacttccag cacccgctcg ccgccggcca gtggatggag atcggcttca cggggttgca
     7861 gtgattccat aacagcattc agcggcagaa tgaaaacttc atccccaacg cgtacggaca
     7921 tgccgtcgag gatggccagc gtcagcggca gtaaaatgcg gatcgtagtg ccagtcccct
     7981 gcttcgactg gatttcgaca tgaccgccca tctcctggat attacgttta acgacgtcca
     8041 tgccgacgcc gcgcccggag acgtcggtga cctgctctgc catggagaag cctggtgcaa
     8101 atatcagcat cgcgacttcg tcgtcgctca tgttttcgct gacagtcaaa ccttgcgagg
     8161 ccgcttttgc cagaattcgc tcacggttta gccccgcccc atcgtcggtc acttcaatgc
     8221 aaatgttgcc gccctgatgt tcggcagaca gaattaaatt tccgacgctg tttttacctg
     8281 cggcgagccg tttttctggc agttcaatac cgtgatcgag gctattgcgt accaggtggg
     8341 tcagcgggtc gataatgcgt tctatcaggc ttttgtcgag ttcagtagaa ctgcccacca
     8401 gcgtcagttc tacctgcttg ccgagttttc ccgccagatc acgcaccagc cggggatagc
     8461 gactaaaaac atattccatc ggcatcatgc gaatcgacat cactgattcc tgcaaatcac
     8521 gggcgttacg ttgtaactgc cccatgctgg ttatcaaatc accatgatta accgggtcca
     8581 gttcgctgga acgctgggca agcatggact gggtgataac cagctcgccg acgaggttaa
     8641 ttaattgatc aaccttttct accgctacac ggatgctggt ggattcattg ctgcgcgtcg
     8701 ttttttcccg ctccacgcgg ccggttggcg cttgttcggc tgccagttta agcactggtg
     8761 gggtggatat ttttggcgag acttctactg tttcaaaggt aatctgatcg gcttcaatca
     8821 caaaacagag taccgctgtg atgtcatctt cggcgatgtc gcccggtaat attgccgaga
     8881 gcgaatccgc ccctttcacc acgtcagtta acgttgtcag atgtcccagt tcttcttcca
     8941 gcaggtcgac ttccccggcc ttcaggcgcg aaaggataat tcgtcgcggc gactgactgc
     9001 gactctgctc atcttgcggt tcacttttgg caaccacact taatcgggtc actgcggatg
     9061 gcgtttcgcc tttcgcttct aatgccagtt gacgcaaggc ctggcagata taatcgaagc
     9121 tggcggcatc cggctcttgc gactgtttat aagcgtcgag ctgttcttgc atgatgtcct
     9181 tcgtttccaa aaacagattg ataatgtcgg tgttgagttg catctcacct cgtctggctt
     9241 catcgagcag gttttccatc agatgcgtgg tttcctgcaa aacgctgaag ccaaaagttc
     9301 ctgcccctcc tttgatcgag tgggcagccc gaaagatggc attcaattgt tcggcatctg
     9361 gcgcttccgg ctgcaaaacc agcagatgct gctccatgtc agccaacagt tcgtccgctt
     9421 catcaaaaaa tgtctgataa aaatcgctta tatccatgct cacgctgtca cctcggttcg
     9481 gctgatggca ttgtgggaac actgacctgt ggtgcaacct caggtttttc cagggcgctt
     9541 actggctcat tctggctttc ggcgttttca tgcaaaatgg cctgttcggc ttgtttgttc
     9601 agtaccagca ggctgatgcg acggttgacg gcatcatcag gtccgcgatc gcttaagcgc
     9661 atcgttgccg ccatgccgac gacacgtaac actttgccgc tatccaaccc tccgaccatc
     9721 agttcgcggc gggatgcatt ggcccgatcg gcagaaagct cccagttgct atatcctttc
     9781 tcaccgctgg cgtaggggaa atcatcggta tgacctgaaa ggctaatacg gttgggaata
     9841 ccgttcagta caggcgcaat ggcacgcaga atgtcgcgca tatagggttc gacatcggca
     9901 ctgccggttc taaacatcgg gcgattctgg ctatcgatga tctgaatacg tagaccttcc
     9961 tggaccagat cgattttgag atggggacgt aacgcccgca gtttcggatc ggactctatc
    10021 aactggtcga gatcaccccg caatttccgc aatcgacttt gctccatgcg ttttttcaga
    10081 tcttcgatgt tcggctgctt attcacttcc ccctggcttt gggtgtaatc atcaccaccg
    10141 ccgggaattg ggctttcact attagaaatg cgatcgccgc ccgtaaccgc agtcgccagt
    10201 ggagtccgga agtactccgc aatctgaatc agctcttttg ggctggagat ggagatcagc
    10261 cacatcacca gaaaaaaggc catcatcgca gtcataaagt cggcataagc aatcttccac
    10321 gatccatgtg ctgccccgtg gcttttggct ttgcgtcgtt tgacgacaat aatcggatgc
    10381 gcttgattct tcatgcttcc tcggttgtcg tctgttgttg cggatttttc accgcacgca
    10441 catgctcttc cagttcaata aacgacggac gttcgctgga atagagcgtt ttgcgaccaa
    10501 actcaacggc gataggcggt gcgtaaccgt tcagattaga aagcagagtg actttgacgc
    10561 actgcatcat tttgctggtt tcggcgcttt tctgacgtaa aacactcgct aatggggaaa
    10621 taaatccgta agccaataaa atgccgagga aagtccccac catcgcatgt gcgataagcg
    10681 cacccagctc ggcggcagga cgatcggctg aacctaacgc gtgaacgccc cccattacag
    10741 ccgcgacaat accaaacgcc ggaagtgagt ccccgaccag cgccagactg ttcgccggga
    10801 cttctgcctc gctttcgtgc gtctcaatct cttcatccat cagggcttcg atttcgaagg
    10861 tgttcatatg accgctgata atcagacgca gataatcgac gataaaatca agcatgacgc
    10921 tatccgcgag gatgcgtggg tagctggcga agatctcgct ctcacgggga ttttcaatat
    10981 cacgttccag cgaaaacatc cccatctgcc gcgatttcgc catcaaccga taaagcagag
    11041 ccagcagatc catatacatt gctttggtgt atttggagcg acgaaacagc aacggcagcg
    11101 ccttcagcgt gcctttaatc gctttgccat tattgccgac gataaacgac ccaatccctg
    11161 caccggcaat aatcaccagt tcagcgggtt gatagagtgc tccaaggctt ccaccggtca
    11221 tcaaataacc gccgaaaact gtaccgagaa caaccaggta acctaataag ataagcacga
    11281 catcatcctt ccactgttga ccatgacagg atgttcagtc gtcaggcgtt aacgcgcgat
    11341 tggggcaaaa aaagcagcgg tacgtcgtta ccgctgctgg aatgttgcgc ctcaccgtat
    11401 cagttaaaca gcctgtactc tctgttcatc cagcagttgt gggataatat cggcaggatt
    11461 ctgggaaagt ttacgtcttt ttactgcccg ggatggcggt tgacataagc tgcaggcaaa
    11521 gctgccaaca ggctggtgag cgtgggtaat aaaattgccg ccgcagcagt tgcagctgga
    11581 aagttgcagt aatccacttt caacaaaccg caccaatgtc caggcacggg ttaatgccag
    11641 cagtggtcct tcttctgctt gtgggcactg ttcaaggtat aaacggtagg ctttgatcac
    11701 cgcatcgacg ccattacaca aaccggtttt cagtaaaaac tgccatgcat tacagaacat
    11761 cgaagcatga acgttttgtt cccaggtcat aaaccagtcg gttgagaatg gcagcatgcc
    11821 tttcggcggt gggcttccgc gcagttcttt ataaagtttt atcaggcgtc cgcgacttaa
    11881 ctgtgtttcg ctttccagca tctgcaaacg agcgcccagg gtaatcaatt ccattgccag
    11941 ctgaatatcc cgcgcttcct gaacaatgct tttttcactc atgatcaggc ccttttcttg
    12001 cgcagcgctt cttcaggctg attaacatca ttcagcaagc gtgttgagag catgatgccg
    12061 gtatgaattt gctggagatc gtcaacgcgg gaatcttgcg tcaactgagt aatcgtctgg
    12121 tggctgtcaa aacggaagtg acaaaccagt tgattggttt ctgccagctt aaccatttgc
    12181 ggaagagtca gtgccgctaa cgttgtcgcc atttcttcat ttatgccgag acgaaacata
    12241 gcggacgctt tgtcctgaac aatcaaacgc tgtgcaagta gtaaatatga caagttgatg
    12301 tcataaatgt gtttcagcaa ctcggaggta tgcattattc ccacccagaa taaccaactt
    12361 tatttttatg cggtttcacc gcaccccgtg atgtcgccgg gaagccccgg taaaaaataa
    12421 ttagcattag aatagttgcg ataagctgca ataagcagaa ccaccttttt ggtttaatat
    12481 gtccttacaa atagaaatgg gtctttacac ttatctaaga tttttcctaa atcgacgcaa
    12541 ctgtactcgt cactacacgc acatacaacg gaggggggct gcgattttca ataatgtgtg
    12601 atgcagatca cacaaaacac tcaattactt aacataaatg tataagtcat acttttgttt
    12661 tgggtgtatt tcaatatgtt aaaaagtttt tcgctacgct agcaagcaaa aatgaaacag
    12721 gaataatcga aatgggatgt tgcgcacagt taaaataaat caccgtaaat aatcatctgc
    12781 tataaataat cactttcatg caataccaga taagctattt ttaaacagac acttaccgca
    12841 caacaaacta attaaataca aagaaaggca acaatcaact taatgttaat gaaatgtttt
    12901 acatatatta accacttgtt taatgcggag aatcagtaca ctattttctt ataattacat
    12961 ttgaaattat atgatcaccg ggagtgattg attaattgtc tttactaata atcagactaa
    13021 tatttacctg tttgaccgag ttgggattgc gtcgtttctc cattaggagt aaagctttaa
    13081 tgtcacctga agttcacaga ataaggaaca ggttatgagc tatagcaata ttcttgtcgc
    13141 tgttgcggtt acaccggaaa gtcagcaact gctggcaaaa gcggtatcta tcgccaggcc
    13201 agtaaaggga cacatcagtt taattactct cgcttccgac ccggaaatgt acaatcaatt
    13261 agccgcgccg atgcttgaag atttacgcag cgtgatgcaa gaagaaacgc aaagctttct
    13321 tgataagtta attcaggatg cggggtatcc cgttgacaag acgtttattg cctacggtga
    13381 gttaagcgaa catattctgg aagtatgtca caaacatcat ttcgatttgg tgatttgcgg
    13441 taatcacaat cacagtttct tttcgcgagc atcctgctcg gcgaaaagag tgattgcctc
    13501 aagtgaggtc gatgtgctgt tagttccact tacgggagat taaccgctcc tacgcaagct
    13561 ttggaaaggt agcaacttta tcgcgctgct ggctttccgc gcttcgcgga actatctgct
    13621 ttaggtcgct aatgaagcac tcctgccagt ggttaatatc gtttttcacg ataacgtcca
    13681 gcatttctgc atgacgggaa atacgttccg ccagcgacat agtcaatgca cgatccagcg
    13741 cagctgcaac ttcgtcacga tcgtaggggt taacaattaa cgccgacgtt aactcgtttg
    13801 ccgctcccgc aaattgcgaa agaacaagaa cgcccgggtt ggctgggtcc tgagcagcaa
    13861 catactcttt tgctaccagg ttcatcccgt cacgcagtgg cgtcactaag cccacgtcag
    13921 agtagcggaa tattttcatc agtaatttac ggtcaaaatg ctgattcaaa taataaagcg
    13981 gcgtccagcc taattgcccg tatttaccat taattcgtcc agcttcattt tcgagctgat
    14041 gacgaatatc ctgataggct tgcacatcac cacgcgacgt tggtgcaatc tgggtataac
    14101 gaattttacc atgatgctgc ggatattttt ccagcaacgc ttcataggcg agaaaacgct
    14161 ctggcaaacc tttggaataa tccagccgtt cgacagaaaa gatattttgt acgtttttca
    14221 gttccgcttt aagttgcgcc agttttggcg gcagtggccc ggcagcctgt ttggctattt
    14281 ctttcggttc aatgccgatc gggtagactt ctgttcgaaa tgctttgccc caggctgtat
    14341 ggctttttgc gctacgtgtc gtgacgcggg tcaggttaga aagacaatcc aggaacgcca
    14401 gacgatcgtt ttctgtctgg aaacccagca aatcataatc acaaagctgt tcaagcaagg
    14461 tgtcatatgt cggcagcgcg ttgaagattt ccggtgtcgg gaaaggaata tgcagaaaga
    14521 aaccaatgcg attattcact ccccgtttgc gtaattcatg cgcaaatggc aacaggtgat
    14581 aatcgtggat ccagataatg tcatcgtctt gcaacagcgg cagtaattta tctgccagca
    14641 acgcatttac gcgtagatag ccgtcccagg caggacgctg aaattgcacc agatcgagcc
    14701 gataatgaaa agcgggccag agaacggcat tggagaattg gttgtagtat tcgtcaaggt
    14761 cctgttcgct gaggttaaaa gaggcccacg taatgttacc ttttttcacc ttttttagcg
    14821 gctgatcctc attccctgtt tcaccactcc agccaaacca cagtccgcct gcggctttca
    14881 gtgcccccag tatgccaacg gcaaggccac cggcactggc ggcgtgctcg tctggtggtg
    14941 caatccggtt agatactacg actaaacgac tcatagtcat cactcctgtt attttctctt
    15001 ttttgttgta atgcggtggt tatcatttca agccagctcc agacatccgg cacacccgcc
    15061 agtcgccatg atgcctgagt tgcacctgtg ccaattttta ctgacattcc gcccagtcgg
    15121 ttaacgactg cgaagccaga ttcatcggtt aaatcatcgc ccagaaatac gggcgttcgc
    15181 ccgataaagg gagcttcctg cataaaagct gcaattgcct cacctttact ggtacctctc
    15241 ggtttgatct cgacaacaca ctttccctgc tgtaacgcca tttgtggcca gatctgagta
    15301 atacgttgcg ctaatgtcat taatgcgtct tcatgctgcg gagcctgacg ataatgcagc
    15361 gcaaaagcca tccctttcgc ctccagctcc gcgccgggat actgagcgat gactgtatgc
    15421 agttgcacgc taatatcacg cgcaatcgca tccggcagat gaacgatatg tgttttacca
    15481 ttgatgtcac ggcgctccgc cccatgcacg cccgctaacg ggaagcgata aggttttgcc
    15541 agtgcgtcaa gctccaccat tgagcgccct gatatcaatg ccaatgcacc atcacttgcg
    15601 gttgccagta gctgtagtcc ttgcagaata ttgtcaggca cgacgacctg atcgggatgc
    15661 ggtttgattt ccgccagcgt tccatcaaga tcaaaaaacc aggcatattt cgcggatagt
    15721 tcaggggttt cggttaacgg ttctgtcacc cggctctcct ccttctcttt tcttattgtt
    15781 ttgcgcagaa cgcgctttac agacatgtaa gtatagacag tgtgacgggg gtcgccattt
    15841 gcaatgaaga acagccaacc gttgtggcgg ctggctgtgg tgggaaaaaa cgttaaattg
    15901 ttgtggaaaa aagcatcaga cagtgcgttt cgctttttgc ttgtaacggt cgaagatcac
    15961 cgctgccagc aggattaagc cgcgaaccac gtactgcgcg aaaggagaaa tattaagcag
    16021 gttcatggcg ttttccacgg tgcctaaaat taagataccc gccaccacat atgagatttt
    16081 tccgatgcca cctttcagag aaacgccacc taaaacgcag gcggagataa caataagctc
    16141 ataaccaatc gacgtcattg gctgcccact agtcatacgt gaagccagaa taattccggc
    16201 tatcgcagat accaggccag agagaacaaa gataataatt ttggtgcgaa caaccggtac
    16261 acccgccaga cgcgcagcct cttcgttccc gccaatcgcc agggtgttac gaccaaaggt
    16321 ggttttattc agcaacaaac caaagataat cagacacgcg acggtgagcc agattggcgc
    16381 aggcagaccg aaccagttag cgtaaccaag ggcaaagaag ctttcatctt cgataccgac
    16441 cgctttaccg tctgaaatga tgtacgccag acctcgaaca atctgcatcg ttgccagtgt
    16501 tgtgatcaga gcatttattt tcagtttggc gataacaaag ccattgacca ggccacagag
    16561 aatgccaagc agcaaccccg ctgccacgcc aatccacagg ctttcagtca ggttgataac
    16621 caccgccgtg gtgacacccg cacaggcaat tacggaggcg acagaaaggt caaagtcacc
    16681 ggaagcaagg cagaacaaca tgccacacgc caccatcccc gacatggaaa ttgccaggcc
    16741 caaccctttc atattaatga aggtggcaaa atttgggaca aaaatggcac aggcgataaa
    16801 gagcaccgca aacaccacca gcatgccgta ctgatcccaa atacgcccga agctgaatga
    16861 cgacttaggt gcgccagaac ccgatgtaga aacagaagac atcatactct ccttactcag
    16921 gcaactgcct ggctgacttt aggcatcgca aggctcagtg cctgacgctc atctgcctgc
    16981 tcgtgtaaca attcaccggc gatttcacct tcccgcatca ccacaatccg gtcggcaacg
    17041 ccgaggactt caggtaagtc gctggaggca aacagcaccg ccacaccctg cgccgccagc
    17101 gcataaatta cgttatatat ttcgtgctta gcaccaacat caatgctgcg cgtaggttca
    17161 tccagcaaaa tgaccttcat ctcttccgat aaccagcggc ccagaatggc tttttgctga
    17221 tttccgcctg agagattcat gatcagttgc tcagcgcccg gcgttttgat gttgagcgaa
    17281 cgaatgtgct gatcggcatt gttttcttcc caaccgttgt tgattacaca accgccgagc
    17341 acatgtttgc gtctggcact gatgttgata ttgtcgcgaa cggagtgcac gggaataatg
    17401 ccttccgctt tgcgatcttc cgggcagagc atcatgcctg cggcaatggc gtggctcggt
    17461 ttacgaatat cgatcggctg ttggtcgata taaacctgac cggcggtgat ttgcgtcccg
    17521 ccaaacaagc ctttcattaa ttcgctacgc cccgccccta ccagaccaaa cagcccaaca
    17581 atttcaccac tgcgaaccgc cagacttatt ggcgtacgca cgcctggtgc tttcacagca
    17641 tcaagacgca ggcgctcctc gccataacta cgaggttgcc agccgtagat atcgccaatg
    17701 tcgcgcccga ccatcgcctg caccagcgcg tcgtggtcaa cctgctgcat atcggtaaag
    17761 gttttgacat aacgtccatc tttaaagaca gtgatggcat cgctgagggc aaatatttct
    17821 tccatacggt gagaaacgta taagatcacc cgcccctctt ttcgcagttc acgaataacg
    17881 cggaaaagat tgtcgatttc gcgggcagaa agggagctgg ttggctcatc aaaggcgata
    17941 attttagcgt tacgcgccag cgccttggcg atttcaacca tctgccactg accaatggag
    18001 agatatttca gcggcgtgtc cgggtcaata tccataccaa gatgtttaag ttgtaaaccc
    18061 gcctcataat tcagcaatga gcgattcaca atgccgcctt tatgcggcag ctggccgaga
    18121 tagatgtttt ccgcgacggt catttccggc acgagatgca gttcctggta aataatcgcc
    18181 acacccgcgt taagtgctgc ggtcgtgtcg gaaaaggaca tttcctgccc attaatcact
    18241 acagaacccg tggttggcgc atagttgccg ctgaggattt ttaagagagt tgattttcct
    18301 gcgccatttt cacccatcaa cgcatgaacc tgaccggcat agcagtcaaa actaatatcc
    18361 gtcagcgcct taacgccggg aaacgtttta ccgatgccgc gaaatgagag atacggggta
    18421 gactgttgca taacgtctcc gtgaatcact ggtcgttcac accccctctg catcatgcag
    18481 aggggaattt ttccggcaaa ttacttaccg cctaaacctt ttttctccag ttcttcttta
    18541 aagttgtcac gcgtgatcag caccacgtcg gtaacttcgg taaattttgg cggttcaacg
    18601 tcttttgcta cccagttgta aagcatttcg ctggatttat agccatgtac gtccgggctt
    18661 ggcagcaggg aaccgtagaa gccggttgcc tgtgctttag acagttcgct caacgcatcc
    18721 acaccgttaa tgccaatgcc gatgatatcg gcagctttaa agccctgacc ttccgtcgcg
    18781 cgtacgccgc ccagcacggt gctgtcgttc ataccgacga tcagccaatg tttaacttcc
    18841 ggatgttgaa ccagcattga gttggcagcg tcaaatgccc ccgggatgtc gttagatttg
    18901 gtaggtacct gataaatttg tttttccggg aatccggccg ctttcagcgc atccatagaa
    18961 cccgttgtac ggcgacgtgc cgtatccagt tcgttggcgg taatcgccat caccgcgctt
    19021 tctttgacat cccagccacg tttctgcatc tctttataca gttcctgacc ctgacgttcg
    19081 ccaattttag tcgccgccat catcaccagc ggaacggtat ccattggctt acctttggcg
    19141 ttaacaaact ggtcatccac ggcaatgact ttcatatcgt agccacgcgc tttcgcgacg
    19201 atggcagagc cgagtttggg gtccggagta caaataacga aaccttttgc gccactggca
    19261 gccaggctgt cgatcgcgtt caatgttttt tcgccatccg gcacggcaat cttaataacc
    19321 tcaaacccta aatccttccc ggctttatcg gcaaacttcc attcggtctg gaaccacggc
    19381 tcttccggtt gcttcaccag aaaaccgagc ttcaggttct ccgccatagc ggattgtgac
    19441 ataacggctg ccagaccaat ggctgccagg gctttagtaa atttgtgcat ggttctctcc
    19501 agctttagtg tcgttttgtg tagggcaaaa acgaatgact ttcgttaaat taatcggaaa
    19561 acaaagcatt accttttatc taaaagataa gtgaccgtgt tgacatagtt ttagcgagaa
    19621 attaattctc cataggagag caatatcaca tcgcagaatt acagtgagaa cgtgcataaa
    19681 tttagcggga aaagacataa gggaaagcca atttgtcaga caaattgtcg aatgcacagc
    19741 agattaatcc ataagattag cctggaaatc cttgttgtct ttggtaccca tgcgggatgt
    19801 cttcttttta accagtcaat aggccgcatt acctggcgtt gagtttttga aatggtgtaa
    19861 taaccgcaac tcaaagatgt ggaaaatgca cgtcattcat ttcgccatta attatcactg
    19921 tgctcattaa ttaacagaac acgtataatg agagccatct cgcaaaaatg aaaaaacgtt
    19981 ttataaaatc atcacttcat catgaattca aattcattga ttaatatcaa caagatacaa
    20041 aaagcactat cattaaaatt cattgcagtt acattgattt catcaatgaa atgtaaaaat
    20101 atataaactt gatgatttaa gcattttctt atacccgttc agacgttatt cttatttcag
    20161 atcatcgtca gaattgactc cacgatcaca tttcggaccg gcagaaagga attattctgc
    20221 aaacagtaat tatggtgttt tgatttatct tgcacctctc cacttctgga tataaggata
    20281 ttaggtatgg caaccgctgg aatgcttctc aaactcaact ctcaaatgaa ccgcgagttt
    20341 tacgcatcca atctctacct tcacctgagt aactggtgtt ctgaacagag tctgaacggc
    20401 accgccactt tccttcgcgc ccaggcacag agtaatgtga cccaaatgat gcgcatgttt
    20461 aactttatga agagtgtcgg cgctaccccc atcgttaaag ccattgatgt tcccggtgaa
    20521 aaactgaact ctctggaaga actgttccaa aaaacgatgg aagaatacga gcaacgttct
    20581 agtacgttgg cacagttagc cgatgaagcg aaagaactga atgatgattc aaccgtcaat
    20641 ttcctgcgcg atctggaaaa agaacagcag catgatggtc tgttgctgca aaccattctt
    20701 gatgaagtgc gcagtgcgaa acttgcgggt atgtgccctg tgcagaccga ccaacatgtt
    20761 ctgaatgtcg tgtcacacca gctgcattga tcatcatcgg cgctaatgca ttgcgccgat
    20821 gaaggttttg agaaaccgct gcctcatctg tttgaagcag cggttttttt aatgggattc
    20881 accctgtggg gtaaacttga gttcaataag cgcgatggct ttttggattg cccgcatggt
    20941 gaccgggtct gcggcggcgg gatggttagt aaagtcgata ttcttcagct gactggacat
    21001 tttttcacga acttcaacgg gcgcgattac atcgagaaca tccagaattt gtttgataac
    21061 caactggcaa gcaaccacat cagaaaccag ttcctgatcg gcattcagcg gctgggacat
    21121 cgtaaactcc tgatagcatt ttgaaagccg ttatagtagc ggcttcacat cttcagcgat
    21181 agtcacatcc accgtcatca ggacacaaaa aaacctgccg gagcaggttt tttgttatcg
    21241 gaacatattg cctggcggta cgtctttgaa cgtcttgcaa tagttattga acatactttt
    21301 caggattttg cgcagtttca tcgcggcact ccgaccattt gttatacagg tgttattgtc
    21361 tttgcgctta taatatgaca accatcacaa aaatcaatct ttatgtgata caaatcacat
    21421 aaatacccct ttaatgttat aaaaatgata atcaaaaaac agccccccta tttctgacac
    21481 ctacagatgg caagaaatag cgcctgccag gcgtcttttc cggccattgt cgcagcgctg
    21541 taacgcgtaa aatagtgctt tctcttactc ttctggctgg accatgagac ttctgattct
    21601 gactctttca ttaataacgc tggcggggtg tacggttact cgccaagccc atgtgagcga
    21661 agttgacgcg gcaacaggta ttgtacggtt ggtttatgac caggcttttt tgcagcatgc
    21721 ccataccgat cgttatgtca gtcgcgggat cgccgatcgc gcatgccagc aggaaggcta
    21781 tacccacgcg gtcccctttg gtcagccagt aggcaactgt agcctttttg cgggttcctt
    21841 atgtttgaat acggaattca ctttgtcata tcagtgccat cattccgcct tccctgtctt
    21901 tctctaaata ttttaatcag cgaggggatc ttcgctgatt aaagaaatag cggtaatgcg
    21961 ttttattccc at
//
LOCUS       DAESHI010000068        21684 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.068, whole genome shotgun sequence.
ACCESSION   DAESHI010000068 DAESHI010000000
VERSION     DAESHI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.068"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..182)
                     /locus_tag="KW011_002640"
     rRNA            complement(<1..182)
                     /locus_tag="KW011_002640"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(560..1105)
                     /gene="hemG"
                     /locus_tag="KW011_002641"
     CDS             complement(560..1105)
                     /gene="hemG"
                     /locus_tag="KW011_002641"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HBH7660943.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(1117..2568)
                     /gene="trkH"
                     /locus_tag="KW011_002642"
     CDS             complement(1117..2568)
                     /gene="trkH"
                     /locus_tag="KW011_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HBH7660944.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2607..3221)
                     /locus_tag="KW011_002643"
     CDS             complement(2607..3221)
                     /locus_tag="KW011_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HBH7660945.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(3221..4552)
                     /gene="pepQ"
                     /locus_tag="KW011_002644"
     CDS             complement(3221..4552)
                     /gene="pepQ"
                     /locus_tag="KW011_002644"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HBH7660946.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4742..6931
                     /gene="fadB"
                     /locus_tag="KW011_002645"
     CDS             4742..6931
                     /gene="fadB"
                     /locus_tag="KW011_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HBH7660947.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLSMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6941..8104
                     /gene="fadA"
                     /locus_tag="KW011_002646"
     CDS             6941..8104
                     /gene="fadA"
                     /locus_tag="KW011_002646"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HBH7660948.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(8294..8995)
                     /gene="fre"
                     /locus_tag="KW011_002647"
     CDS             complement(8294..8995)
                     /gene="fre"
                     /locus_tag="KW011_002647"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HBH7660949.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(9042..10535)
                     /gene="ubiD"
                     /locus_tag="KW011_002648"
     CDS             complement(9042..10535)
                     /gene="ubiD"
                     /locus_tag="KW011_002648"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HBH7660950.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10702..11190
                     /gene="rfaH"
                     /locus_tag="KW011_002649"
     CDS             10702..11190
                     /gene="rfaH"
                     /locus_tag="KW011_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HBH7660951.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(11187..11969)
                     /gene="tatD"
                     /locus_tag="KW011_002650"
     CDS             complement(11187..11969)
                     /gene="tatD"
                     /locus_tag="KW011_002650"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HBH7660952.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(12011..12787)
                     /gene="tatC"
                     /locus_tag="KW011_002651"
     CDS             complement(12011..12787)
                     /gene="tatC"
                     /locus_tag="KW011_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HBH7660953.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12790..13305)
                     /gene="tatB"
                     /locus_tag="KW011_002652"
     CDS             complement(12790..13305)
                     /gene="tatB"
                     /locus_tag="KW011_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HBH7660954.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(13309..13578)
                     /gene="tatA"
                     /locus_tag="KW011_002653"
     CDS             complement(13309..13578)
                     /gene="tatA"
                     /locus_tag="KW011_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HBH7660955.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13657..15297)
                     /gene="ubiB"
                     /locus_tag="KW011_002654"
     CDS             complement(13657..15297)
                     /gene="ubiB"
                     /locus_tag="KW011_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HBH7660956.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(15294..15899)
                     /gene="ubiJ"
                     /locus_tag="KW011_002655"
     CDS             complement(15294..15899)
                     /gene="ubiJ"
                     /locus_tag="KW011_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HBH7660957.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15913..16668)
                     /gene="ubiE"
                     /locus_tag="KW011_002656"
     CDS             complement(15913..16668)
                     /gene="ubiE"
                     /locus_tag="KW011_002656"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HBH7660958.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16763..18190)
                     /gene="rmuC"
                     /locus_tag="KW011_002657"
     CDS             complement(16763..18190)
                     /gene="rmuC"
                     /locus_tag="KW011_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HBH7660959.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(18331..19092)
                     /gene="udp"
                     /locus_tag="KW011_002658"
     CDS             complement(18331..19092)
                     /gene="udp"
                     /locus_tag="KW011_002658"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HBH7660960.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            19354..20166
                     /locus_tag="KW011_002659"
     CDS             19354..20166
                     /locus_tag="KW011_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBH7660961.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            20560..21621
                     /gene="hcp"
                     /locus_tag="KW011_002660"
                     /pseudo
     CDS             20560..21621
                     /gene="hcp"
                     /locus_tag="KW011_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
BASE COUNT         5320 a         5805 c         5417 g         5142 t
ORIGIN      
        1 ttatgcggta ttagctaccg tttccagtag ttatccccct ccatcaggca gtttcccaga
       61 cattactcac ccgtccgcca ctcgtcagcg aagcagcaag ctgcttcctg ttaccgttcg
      121 acttgcatgt gttaggcctg ccgccagcgt tcaatctgag ccatgatcaa actcttcaat
      181 ttaaaagttt gatgctcaaa gaattaaact tcgtaatgaa ttacgtgtcc actcttgaga
      241 cttggtattc atttttcgtc ttgcgacgtt aagaatccgt atcttcgagt gcccacacag
      301 attgtctgat aaattgttaa agagcagtgc cgcttcactt tttctcagcg gcgcggggtg
      361 tgcataatac gctttcccgc tacagagtca agcatttatt tttgctttct ctgtcggaat
      421 tctcaggaga accccgctga cccggcggcg tgtttgccgt tgttccgtgt cagtggtggc
      481 gcattatagg gagttattcc ggcctgacaa gaggaaattt aaaataattt tctgaccgcg
      541 cattttttat tctttatgct tatttcagcg tcggtttgtt ggttaactgg gcgatttctc
      601 gggcgaaatt cgccacctgc tcccaatcgg tatagacaac ttctttgcgc gtatccgttt
      661 caccgcctga catcttcata attagcttga tcataaaacg atcgtaccag cgataacgtg
      721 ggtaacgcag cgccccggca atgaccgcgc agcgatcggg acgccattgc gagttcatca
      781 gaaacttccg cgcgtagctg ttggtctgtg gagtacgctt ctccggtttg cgcgccacca
      841 ggtttacgga ataaaaggcg ctcggcatcg aattcagtcg cgtcgcatgt tttttgacaa
      901 attcctggaa cgcagagtgg tagtgaccat agcgaataga agcaccaatg accacacggt
      961 cataattttc ccattgtggt tcttcaatgc ggtgcacatt ggcgacatcc gtctggatcc
     1021 ccagttcttt cagttccgaa gccaggtagg aggcaatctc gcgcgtttgt ccgtcccttg
     1081 ttgagaaaag aattaatgtt ttcacgtatt actccatcat tcacgccaga aagtcggggt
     1141 aaagagcacc agcaatgtaa agacctcgag acgaccaaac agcatgttgg caatcaggat
     1201 ccatttagcc accgggttca tactggtaaa gttatcagca accacgccaa gccctggccc
     1261 caggttattc aatgtcgcaa caactgaagc aaaggcagaa aagtcatcca cgcccgtggc
     1321 gataatcgcc agcatactga caataaacac caatgcatag gcggagaaaa atccccaaac
     1381 ggcttcgagg atacgttccg gcagtgcgcg attccccagc ttaatgctat agacggcgtt
     1441 cggatgcacc agtcgtttca gctcacggtt cccctgctta aacagcagca ggatgcggat
     1501 cactttcagg ccaccgcccg ttgacccggc gcaaccgccg ataaaagctg aacataaaag
     1561 cagtaccggc aaaaagagcg gccagcgggc aatgctgtca gtcgtaaacc cggcggttgt
     1621 cgccatcgat accacctgga aaaacgcctg gttaattgtc atcagcgccg aactgtagac
     1681 attatgaaac cacagtacga gtgtacaaat aaccaccagc gtaaactgta cgccgataaa
     1741 catgcgaaat tccggatcgc gccaataaac cttcagacta cgcccactta acagtgaaaa
     1801 gtgcagaccg tagttacagc cggagatcag caggaagata gcaatgatag tgttaatagt
     1861 cgggctgtcg aaataaccga tactggcatc atgtgtcgag aagccgccaa tagcgatagt
     1921 cgcaaagcta tggccgatgg catcaaaggc atccattccg gcaaaccaca acgccagcgc
     1981 acaggcgacg gtcagcaaga cataaatcaa ccacagggtt ttcgccgttt ccgcaattcg
     2041 cgggcgcatt ttgttatctt tcagcgggcc gggcatttct gcgcgataga gctgcatccc
     2101 acccacgccg aggataggca gtatcgcaac cgctaacacg atgatcccca tcccgccaaa
     2161 ccattgcagc atctggcgat aaaagaggat ggcatgaggg agcgaatcca gccccaccag
     2221 cgtagtggcc ccggtggtgg tcaggccaga gaaagattca aaaaacgcat cggtaatcgt
     2281 gaggttcggg ctttccgaga agataaaagg gagcgcaccg acactaccca gcacggtcca
     2341 gaacagcacc actatcagaa acccctcacg ggatttcagt tcgcccttct ctttacggtt
     2401 cggccaccac agcatagaac caatggcgag ggcgacaaaa aaggtctggg taaaagcgcg
     2461 gcccgctcca tcccggtaga tgagtgctac cagcccaggg ataatcatgg tccctgaaaa
     2521 taagatgacc agtagtccaa cgattcgggt aatggcgcga aaatgcatct ctgccgcttc
     2581 cttagttctg caaaacgaag tggggattat tcttcaatcg ctaacaattg caatgaacca
     2641 cggctaaaat ccgccagctt tgcggaaaat tcagccactt tcgccgccgg aagcgccacc
     2701 cgcagcagaa cgaatgcctg ataatcactg ttgataattt tgccgtcaca ctgccccagc
     2761 aacgcttcaa tgccggttaa ctgatgatat tcacattgca aagtatattc ggttaatggc
     2821 gtcttgcgtt gggtcgttag ctggcgcagc gcctgattca cgccgccgcc atacgctttc
     2881 actaacccac cggtgccaag caatatgccg ccgtagtagc gcactaccac agcggtaatt
     2941 tccccgacgc cgctgcccat tagctgggcg agcatcggtt tacctgccgt tcctgccggc
     3001 tccccgtcgt cagagaaacc cagctgttga gaatcatccg gcgcacccgc cacccacgcc
     3061 acgcaatggt ggcgggcatc ggggtgttct gcccgcaccg attcaacaaa cgctttcgcc
     3121 gcctcaacgc catcggtatg cgccaacatc gttatgaagc ggcttttttt gatctcttca
     3181 acgaccgtga ccggtgccgc aggaattaac cagctttcca tcacgccagt ttcagatccc
     3241 gggtcatgtt ttccacgttg ttttcgtgga tcaccacgtt gtcttcgata cgaatgccgc
     3301 cgaacggttt cagtgcttca attttctgcc agttgaagtg cttgctgaac tgcccttcac
     3361 gccacggtgc cagtagcgat tcaatgaagt agatacccgg ttcgatggtt aacaccatac
     3421 ccggctggag aatacgggtg cagcgcaggt acggatattt tgccggtgcc gcgaggtgtg
     3481 taccgctatc atcctgcata aaaccggcta cgtcatgcac ctgcaggccc agcggatgac
     3541 cgataccatg cggcataaac ggtccggtga gatcgttttc gaccatcgct tcttcactca
     3601 tatcggtgat gatttgatgt ttacgcagca gtttggcgat gcgctgatgg aactggatgt
     3661 ggtaatccac atagctaacg cctgctttca tggtggcgat cagcgccagt tgttcatcat
     3721 ttacgtcttt caccagctgt gcgtagtcgt tgtcgctttt tgccgaccag gtacgagtca
     3781 ggtcagccgc atagccgtta tattcggccc cggcatccag caggaagctg cgcatctctt
     3841 ccggtgcctg atgatccagt ttggtgtaat gcagcaccga agcgtgttcg ttaagcgcca
     3901 caatgttgct gtaaggtacg tcggtatcac gatgaccggt cgcggtcaga taggcgatgt
     3961 tgatatcgaa ctcgctcatg ccagaacgga acgcttcttc tgccgcacga tgaccgttga
     4021 ccgccatttt ctgcgcttca cgcatacagg ccagttcgta ctcggttttg aaggagcggt
     4081 agtaatgcag gtagtcgatc acccctttcg ggttgatgtt gctggcctca atacccagtt
     4141 gcagcgcacg ttccggcacc ggaccgatat aaccgatatt gccgcgcgca gcgggcagca
     4201 ggctaccaat gccatcggct ttcggcagtg cgatcacttc cacatcttca gtccagaagg
     4261 aggtcggcag cggttcgacg ttgtgccagt aatcaaccgg cagatagaac cacagtttcg
     4321 gcttgttcac gccatccacc agcaaccagc agtttggcac ctgagttacc ggcacccacg
     4381 ctttgaattg cgggttcact ttaaacggat agggatgatc gtcgagaaaa acgttgaaca
     4441 gctcgccgga gtgaataagt aatgcatcca gcttgaagcg cgccagcgca tcgcgagtcc
     4501 gttcttgtaa ggtagctata tgatttttat agagcgaggc cagtgattcc attttttacc
     4561 cttctgtttt tttgacctta agtctccgca tcttagcaca tcgttcatcc agagcgtgat
     4621 ttctgccgag cgtgatcaga tcggcatttc tttaatcttt tatttgcata tttttaacac
     4681 aaaatacaca cttcgatgca tctggtacga ccagatcacc ttgcggattc aggagactga
     4741 catgctttac aaaggcgaca ccctgtacct tgactggctg gaagatggca ttgccgaact
     4801 ggtatttgat gccccaggtt cagttaataa actcgacact gcgaccgtcg ccagcctcgg
     4861 cgaggccatc ggcgtgctgg aacagcaatc agatctaaaa gggctgctgc tgcgttcgaa
     4921 caaagcagcc tttatcgtcg gtgctgatat caccgaattt ttgtccctgt tcctcgttcc
     4981 tgaagaacag ttaagtcagt ggctgcactt tgccaatagc gtgtttaatc gcctggaaga
     5041 tctgccggtg ccgaccattg ctgccgtcaa cggctatgcg ctgggtggtg gctgcgaatg
     5101 cgtgctggcg accgattatc gtctggcgac gccggatctg cgcatcggtc tgccggaaac
     5161 caaactgggc atcatgccgg gctttggcgg ttctgtacgt atgccacgta tgctgggcgc
     5221 tgacagtgcg ctggaaatca ttgccgccgg taaagatgtc ggcgcggatc aggcgctgaa
     5281 aatcggtctg gtggatgggg tagtcaaagc agaaaaactg gttgaaggcg caaaggcggt
     5341 tttacgccag gccattaacg gcgacctcga ctggaaagcg aaacgccagc cgaagctgga
     5401 accgctaaaa ctgagcaaga ttgaagccac catgagcttc accatcgcta aaggaatggt
     5461 cgcgcaaacg gcggggaaac attatccggc ccccatcacc gcagtaaaaa ccattgaagc
     5521 tgcggcccgt tttggtcgtg aagaagcctt aaatctggaa aacaaaagtt ttgtcccgct
     5581 ggcgcatacc aacgaagctc gcgcactggt cggcattttc cttaacgatc aatatgtaaa
     5641 aggcaaagcg aagaaactca ccaaagacgt tgaaaccccg aaacaggctg ctgtgctggg
     5701 cgcaggcatc atgggtggcg gtatcgctta ccagtccgcg tggaaaggcg tgccggttgt
     5761 catgaaagat atcaacgaca agtcgttaac cctcagcatg accgaagccg cgaaactgct
     5821 gaacaagcag cttgagcgcg gcaagatcga tggtctgaaa ctggctggcg tgatctccac
     5881 gatccaccca acgctcgact acgccggatt tgaccgcgtg gatattgtgg tagaagcggt
     5941 tgttgaaaac ccgaaagtga aaaaagccgt actggcagaa accgaacaaa aagtacgcca
     6001 ggataccgtg ctggcgtcta acacttcaac cattcctatc agcgaactgg ccaacgcgct
     6061 ggaacgcccg gaaaacttct gcgggatgca cttctttaac ccggtccacc gaatgccgtt
     6121 ggtagaaatt attcgcggcg agaaaagctc cgacgaaacc atcgcgaaag ttgtcgcctg
     6181 ggcgagcaag atgggcaaga cgccgattgt ggttaacgac tgccccggct tctttgttaa
     6241 ccgcgtgctg ttcccgtatt tcgccggttt cagccagctg ctgcgcgacg gcgcggattt
     6301 ccgcaagatc gacaaagtga tggaaaaaca gtttggctgg ccgatgggcc cggcatatct
     6361 gctggacgtt gtgggcattg ataccgcgca tcacgctcag gctgtcatgg cagcaggctt
     6421 cccgcagcgg atgcaaaaag attaccgcga tgccatcgac gcgctgtttg atgccaaccg
     6481 ctttggtcag aagaacggcc tcggtttctg gcgttataaa gaagacagca aaggtaagcc
     6541 gaagaaagaa gaagacgccg ccgttgaaga cctgctggca gaagtgagcc agccgaagcg
     6601 cgatttcagc gaagaagaga ttatcgcccg catgatgatc ccgatggtca acgaagtggt
     6661 gcgctgtctg gaagagggca ttatcgccac tccggcggaa gcggatatgg cgctggtcta
     6721 cggcctgggc ttccctccgt tccacggcgg cgcgttccgc tggctggaca ccctcggtag
     6781 cgcaaaatac ctcgatatgg cacagcaata tcagcacctc ggcccgctgt atgaagtgcc
     6841 ggaaggtctg cgtaataaag cgcgtcataa cgaaccgtac tatcctccgg ttgagccagc
     6901 ccgtccggtt ggcgacctga aaacggctta aggagtcaca atggaacagg ttgtcattgt
     6961 cgatgcaatt cgcaccccga tgggccgttc gaagggcggt gcttttcgta acgtgcgtgc
     7021 agaagatctc tccgctcatt taatgcgtag cctgctggcg cgtaacccgg cgctggaagc
     7081 ggcggccctc gacgatattt actggggttg tgtgcagcag acgctggagc agggttttaa
     7141 tatcgcccgt aacgcggcgc tgctggcaga agtgccgcac tctgtcccgg cggttaccgt
     7201 caatcgcttg tgtggttcat ccatgcaggc actgcatgac gcagcacgaa tgatcatgac
     7261 tggcgatgcg caggcatgtc tggttggcgg cgtggagcat atgggccatg tgccgatgag
     7321 tcacggcgtc gattttcacc ccggcctgag ccgcaatgtc gccaaagcgg cgggcatgat
     7381 gggcttaacg gcagaaatgc tggcgcgtat gcacggtatc agccgtgaaa tgcaggatgc
     7441 ctttgccgcg cggtcacacg cccgcgcctg ggccgccacg cagtcgggcg catttaaaaa
     7501 tgaaatcatc ccgaccggtg gtcacgatgc cgacggcgtc ctgaagcagt ttaattacga
     7561 cgaagtgatt cgcccggaaa ccaccgtgga agccctcgcc acgctgcgtc cggcgtttga
     7621 tccagtaagc ggtacggtaa cggcgggtac atcttctgca ctttctgatg gtgccgcggc
     7681 catgctggtg atgagtgaaa gccgcgcccg tgaattaggt cttaagccgc gcgcccgtgt
     7741 gcgttcgatg gcggtcgttg gttgtgatcc gtcgattatg ggttacggcc cggttcctgc
     7801 ctcgaagctg gcgctgaaaa aagcggggct ttctgccagc gatatcggcg tgtttgagat
     7861 gaacgaagcc tttgccgcgc agatcctgcc gtgcattaaa gatctcgggc taatggagca
     7921 gattgacgag aagatcaacc tcaatggtgg tgcgatcgcg ctgggtcatc cgctgggttg
     7981 ttccggtgcg cgtatcagca ccacgttgct gaatctgatg gaacacaaag acgttcagtt
     8041 tggtctggcg acgatgtgta tcggtctggg tcagggtatt gcgacggtgt ttgagcgggt
     8101 ttaaccactt gccggatgcg gcgtgaacgc cttatccggt ctaccgatcc tgcaccaatg
     8161 taggcctgat aagacgcgcc agcgtcgcat caggcatcgt gcaccgattg ccggatgcga
     8221 catagatcta aatgccactg aataaagttt tagttgccgt tcttcccgcc tgtcaggggc
     8281 gggtttttat atctcagata aatgcaaacg catcgccaaa cagacgatct tcccgcgcat
     8341 tacgctcact gcaaaacagg tcacgagcaa ttttcgccat ctcaaaacgt ccggcaatat
     8401 agatatcatg ctctgccagc gtaccatgat cctgcaagac cgccgttaac acggtgccag
     8461 tacgcccacg ccagcccgct tccggttgtt caaccaccgg caccacttgc agaccaggat
     8521 gcttcagcga aagcgcctca agctcgcaga gatcatacag atgttgctct tcacgcccac
     8581 cccagtaaat ggtgatatcg cgattgggat tacgcgccaa cgccgtcaat aaaatcgagc
     8641 gcgcatagga gaacccggtg ccgcccgcaa tcaaaatcat cggacgctct tcatcatcgc
     8701 gcagccacgc ttctccgtgg ggaatatcga ccacgatttg atgatctttg aggatgcggt
     8761 ccatgactgc tttcgcgtaa aggttgattt cagaagcgcc aatatgcagc tcgataaacc
     8821 ctttttcatc cggcgtcgaa gccattgaaa acggacgttt gtcgcgctca tccatcacta
     8881 ccatcaaata ctgaccagca cgaaaagaaa aggccgcgtc tggtacgatt cggacacggt
     8941 atacagtatc cgtgatagct tctaccgagg tcactttaca gcttaaggtt gtcatgcgct
     9001 ttctctgtcg gatcaaataa atagggcaaa actaacgcgc atcaggcgct tttaccgttg
     9061 ttaaaaatag ccagttcatc ccagatggcg tcaatatgcg cgacgacatc aggatctttt
     9121 ttgatgggac gtccccattc acgctgggtt tcccccggcc atttattcgt ggcatccagc
     9181 cccatttttg aacccagccc ggagacaggc gaggcaaaat ccagataatc aataggcgta
     9241 ttttctacca gaacagtatc ccgcgccggg tccatacggg tggtaatcgc ccaaatcaca
     9301 tcgttccagt cgcgtgcgtt gacgtcatca tcgcaaacga tcacaaattt agtgtacata
     9361 aactggcgta agaacgacca gacgcccatc atgacgcgct tcgcgtgtcc ggcgtactgt
     9421 tttttgattg tcactaccgc cagacgataa gaacagcctt ccggtggcag gtaaaaatcg
     9481 acaatttccg ggaactgttt ttgcagaatc ggcacaaaca cttcgttcag tgcgacaccc
     9541 agcaccgcgg gttcatccgg cggacgacca gtataggtgg agtggtaaat cgcatcttca
     9601 cgctgggtaa tatgcgtcac ggtaaatacc gggaaactat cgacttcgtt atagtaaccg
     9661 gtgtggtcgc catacggccc ttccggcgca gtttcgcctt gttcgatata cccttccagc
     9721 acaatctccg cactggcggg cacttcaagg tcattggaga tacacttcac cacttcggtc
     9781 ttggtaccgc gcagcaatcc ggcaaacgca tactctgaaa gtgtatccgg aacgggggtg
     9841 actgcaccga gaatcgtggc gggatcggca cccagcgcca cagaaaccgg gaaacgttcg
     9901 cccggatgcg ccgcacacca ctcctgataa tccagcgcgc cgccgcgatg cgacagccag
     9961 cgcataatca gtttgttttt accaatcagc tgctggcgat aaatgcccag attctgccgc
    10021 tctttatgcg ggccgcgcgt tacggtcagc ccccaggtaa tcagcggcgc ggcatcttcc
    10081 ggccagcagg tcataatggg aatgcgattg agatcgacgt catcgccaga gacgattttt
    10141 tgttggcagg gcgcaccacg cagccgcttt gtcggcatgt tcaatacttg cttaaactgc
    10201 ggcagtttat caaacaggtc gcggaaacct tttggcggct cgggctcttt cagaaacgcc
    10261 aataatttac caacttcacg cagcgccgaa acatcttcct gccccatgcc catcgccacg
    10321 cgctttggcg taccgaacag gttgcacagc accggcattg agtagccttt agggttttcg
    10381 aacaacagcg caggcccacc ggcacgcaaa gtgcggtcag caatttcagt gatttccaga
    10441 tgcggatcca ccgggagcgt gatacgtttt agctcaccct gctgttcaag cagcgtcaag
    10501 aagtcgcgta aatcgttata tttcatggcg tccattgtag cctcttaatc tacgcccatt
    10561 atacggcgtt catctttgcg atgctgtaaa tttgttaaat tagcgtgaac tctgacggta
    10621 taacgcaaac cggggaatat aattaactta gcgtaaagct tttgctatcc ttgcgccccg
    10681 attaaacgga taagagtcat tatgcaatct tggtatttac tgtactgcaa gcgcgggcaa
    10741 cttcaacgtg cccaggaaca ccttgagaga caggctgtga attgcctggc accgatgatc
    10801 accctggaaa aaatcgtgcg tggaaaacgt actgcagtca gtgagccatt gtttcccaac
    10861 tacctgtttg tcgaatttga tccagaagtg attcatacca cgactatcaa cgcgacccgc
    10921 ggtgtcagcc acttcgtgcg ctttggcgcg tcgctagcga tagtcccatc ggcggtgatt
    10981 catcagctat cggtatataa accgaaagac attgtcgatc cggcaacccc ttatccggga
    11041 gataaggtga ttattaccga aggcgcgttc gaaggctttc aggccatttt caccgaaccc
    11101 gatggtgagg ctcgctccat gctattgctt aatcttatta ataaagagat taagcacagt
    11161 gtgaagaaca ccgagttccg caaactctaa aacgcaatcc caaaccgtgt tttgacatta
    11221 gcatccgtgg tggcagccag ccatgcggca tcttctccac gccagtgcgc aatacgttgc
    11281 aaaatatggg gcagatgggc tggctcgttg cgccgggatg atggctttgg cgtgagatcg
    11341 cgagggagca gatacggcgc atcagtttcg atcagtaatt tttccgccgg aatcaacggc
    11401 aaaagttccc gcagctccag tccgcgtcgt tcatcgtaaa cccaaccggt aatgccgata
    11461 taaattccat gcgccacgca cgcctgcatc tcttcgcgtg tgccggtaaa gcaatgaaga
    11521 accgcaccag gcagtttatc cagccacggc tccagcaatg tcataaaccg ctcgtgggca
    11581 tcgcgacagt gcataaatac cggcatgttt aaatctgcgg caatgcgtag ctgggcaaca
    11641 aaagcgcgtt cctgctcttc cggcgtcgaa aagttgcggt taaagtcgag accacattca
    11701 ccaatcgcca ccacttctgg ctgcgcggcc agctcaataa tcgcttcttc agtcgcagct
    11761 tgccactggc tgctgtcgtg aggatgtacg cccgccgttg accaacagga cgaatactga
    11821 cgcgccagct tttgcgcctg ctggctttca cgcaggttag tgccggtgat gagtagccca
    11881 ttaactcccg cgtcaaaagc gcgcgctaca acatcatcac ggtctttcgc aaattgcgaa
    11941 ctggtcaaat taacgccgat atcaaacatc ctgtactcca tatgacaacc gccctgacgg
    12001 gcggttgaat ttattcttca gttttttcgc tttctgcttc agcgtcgttt tcctcttccc
    12061 gatttcgccc tttaccaacg taaaagcgtg agaagaagac accgatttca aacagacagt
    12121 acatcgggat cgccaatagc gtttgcgaga agacatccgg cggcgtcagc aacatcccga
    12181 caacgaatgc accaaccagc acatacgggc gttttttgcg taagtcttct ggcgaggtaa
    12241 tccccatcca gcacagcagc acaattgcca ccggcacttc aaaggagaca ccaaacgcca
    12301 taaacagcgc cataacgaag cttaaatagc tggcgatgtc ggtggatacc tgcacccctt
    12361 ccggcgcggt attggcaagg aagccaaatg ccagcggaaa gaccacaaag taggcgaatg
    12421 ccatgccgat ataaaacagc agagagctgg aaaccagcag cggcaccacc aggcgacgtt
    12481 catgcttata cagcgctggg gcgataaatg cccacacctg atagagaatc accggcgctg
    12541 acagaatcag cgacaccata aaggtcagct tgatcggcgt aaagaacggc gaggccacgt
    12601 cggtggcgat catcgttgaa ccttgcggca actgcttgat caatggcgcg gataccaggt
    12661 gatagatgtc attggcgaaa tagaccagac acaggaatat cacgatcacc gcgataatgc
    12721 agttcagcag acgcttacgc agctcaatta gatgcgtgat aagcggttga gtatcttcta
    12781 cagacatgtt tacggtttat cactcgacga aggggaaggt gcagcggttt tcggttcagc
    12841 gtccgcagca ggttttacca ccggctctgg cgtggtttct ggcttctgtt ccggcgaact
    12901 ggcctgcgtt tgtgcagcgg caggcgttac gccctcatgc gcagcttcat tatctttcac
    12961 caccgggtta tggatggtgt gcgcttcatc gctcgccttt tcaggatcgt ttgcaacgta
    13021 ggaacgcttc atcgactccg cggcctggcg tagttcatcc atcgacgctt tcagttcggg
    13081 cgtcaggtta gtgaggctcg ccttttcaac ctttttcaga ctgtcctgaa actcctggag
    13141 ttttaactcc tgggtcagtt cgttctgcac cgttgtcgcc agtgaacgca acgcgcgaat
    13201 ccagcccgct accgttttta ccgccacagg cagtcgttgc ggccccagaa cgacgaggcc
    13261 gatgatgaac accaatagca gttcgctaaa accgatatca aacacggatt acacctgctc
    13321 tttatcgtgg cgcttcgcgt cttctgtttt agcctgttcc tgattcgtat ccgcctgctt
    13381 atcggcgata gttttcgcag taaaatcagc atcctgactg gttttatcct gctttggttc
    13441 atcatcgctc attgcttttt taaagccttt gatcgacgca ccaagatcgg aaccgatgga
    13501 gccgagcttt ttggtgccaa aaagcagtac aacgatgacg gcaataatca ataactgcca
    13561 aatactgata ccacccatac atgttcctct gtggtagatg atgattaaac aaggccgcat
    13621 tatacgttac acgacccgcc ttgagcgatg aaaaaatcag cgtgttttgc gccaaccgac
    13681 aaaccaagcg atcagaccac ctgccattaa ccagccgggc atcagccccc attcaggtcg
    13741 gctgaccaac aagaatgtgc cacttaatac taacgtagcg ccaattccga gaaaataacg
    13801 cgattgtccc tgacgtacat gatttgactg aagctcgcgg gcaatcttat caacactgtg
    13861 ctgtaaatac ttgccctggc gcaaactgtc gtaaaccaat tcaggcagtt ctggcatttt
    13921 ttcgacccag aacggcgctt tttctttaaa tgctctcacc agggcaggaa taccgacctg
    13981 atctttaatc cacgactcca ggaaaggctt cgctgttttc cataaatcga gctgcggata
    14041 aagctggcgt cctacccctt cgacatagag cagggttttc tggagtaaca ccagttgcgg
    14101 ctgcacttcc atattgaagc gacgcgccgt attaaacaga tttaacagta catgtccaaa
    14161 cgaaatttcg gccagcggtt tctcaaagat aggttcacag accgtacgaa tggcaaattc
    14221 gaactcttca acgttggtat ctggtggcac ccagccagaa tcgacgtgta gctctgccac
    14281 tttgcgatag tcgcgattaa agaaggcgat aaagttttct gccagatagc gtttatcttc
    14341 tttgtttagc gagccaacaa tcccgcaatc aatgccgata tatttcgggt tttccgggtg
    14401 ttcatagctt acgaaaatgt tgccagggtg catatcggca tggaaaaagc tgtcgcgaaa
    14461 gacctgagtg aagaacacct gcacgccacg ttccgccagc aatttcatgt tggtgccatt
    14521 tttctccagc gtcgcaacat cagacaccgg aatgccgtaa atgcgctcca tcaccatcat
    14581 cccttcacta caatagtcag ggtaaacttc cgggatgtag agcatcgggc tgtcttcaaa
    14641 atttcgccga agctgaatgg cgttggcaga ttcccgcagc aaattcagtt catcaatcaa
    14701 ggtcttttcg tactcgcgca ccacttcggt tgggcgcaga cggcgaccat ccggcagcaa
    14761 acgcggcacc cagcgagcca gacggtagat aagtttcaga tccgctttaa taaccggcaa
    14821 aatatccggg cggatgactt taatcaccac ctctttacca ttcgatttca atcgcgcggt
    14881 atgaacctgg gcgatagaag cagaagccag tggcttgatt tcaaaatcgt caaaccacgc
    14941 ttctaccggc aagccgccca ttgcagcttc aatctgctgc ttcgccagct tgccatcaaa
    15001 cggagcaact ttgtcctgca ataacgccag ctgatcggca atatgcggtg gaaaaagatc
    15061 gcggcgggtt gataacattt gcccgaactt gatccaaacc ggccccagtt cttgcagggc
    15121 cagtcgtagt cgctcaccta aaagtttgtc tttatgccga tttggcatcc agaataatga
    15181 gtatcgccat agccgtagcg gcagggtgat acgcattttg gggatcagtt catcaagtcc
    15241 gtagcttaaa aaagtgcgaa tgatgaaata taggcgccgt acttcacctg gcgtcattta
    15301 gcctccagtt tttccagccg tttggtcagg gcatcaacag cacgctcgac ggcagccgtc
    15361 tcttccgcaa accaggccac ttcaagcgga ccgggtgcca tacgccactc ttcagtaatg
    15421 gcttccgcca cataacgttg ctggcgctta atgccgtgat gcaggaactt tgcgcctccg
    15481 cgcatggctt tgctgattcc ttcagcggcg atatcaccgg tataaggggc cagcagttcc
    15541 gcagggtcga actctgccag atctgccagc gcaacgaagt tttgcaccac ctgaatatcg
    15601 ccctgcactt ccagctcacc actgcgaatc agtgcggtaa gctgctggcg atcgcgaagt
    15661 ttcggcaaca cactggcgta ggcgataacg gtgcagtcag catcgcctgc ccattcgccc
    15721 agtacatcaa cctggcgttc gctgaacacc agaatcaatg acgtcgaaaa gccttttacc
    15781 tccacgcgca atactttacc cagcagacgc gagcgggctg ttttcagcgc gggtgagcga
    15841 tacaggaagg tgttgagcag actttcaatt cctgccgtca ctaaaggttt aaaaggcatt
    15901 tccggtctcc tgtcagaact tataaccacg atgcagcgcc acaacccctg ccgtcagatt
    15961 gtagtagtcg acactttcga atccggcatc ctgcatcatg gctttcaggg tatcctgatc
    16021 gggatgcata cggatggatt ctgccagata acggtagctg tcggcgtcgt tcgcgaccag
    16081 tgagccaata cgcggcagca catggaagga gtatgcatca taggctttgc tcagcggctc
    16141 gataattggc ttcgagaact caagcaccag caggcggccg ccgggtttca gcacgcgata
    16201 cattgaacgc agtgctttat ctttgtcggt gacgttacgc agaccaaacg aaatggtgat
    16261 gcaatcaaag gtgttatccg ggaacggcag cgcctcagcg ttcgcctgaa catactcaac
    16321 gttgccaatc acaccgatat tacgcagctt ctcgcgtccc attttgagca tggattcatt
    16381 gatatcagca aggaccactt tgccagtttc tccgaccagg cgggagaatt tcgctgtcag
    16441 gtcgccggtg ccaccagcca gatccagcac ggtctgccca cggcgtacgc cgctgcaatc
    16501 aatcgtgaat cgcttccaca aacgatgaat accaaatgac atcaaatcat tcatgacatc
    16561 gtattttgat gccacggaat ggaaaacgtg ggcgaccata tccgcttttt gttccttcgc
    16621 gacggtctga aaaccaaagt gcgtcgtttc ttgtgactta tccaccatct caatgcctgc
    16681 tcatcaaaaa attgttccag aagtgtaaca gattgggcgt cgatgcccta gatttctacc
    16741 cggcttaact acccctaatg ggctagcgcg actgctgatt atattcatca tcgcgttgat
    16801 aagcttcatc attcggctgc tccggaaccg accgaagtcg atactcttca ttctggctca
    16861 ccgcctgttc agccaaatcc ggattaatct cgcgtttaat ttctactcct aaaccgcgaa
    16921 acgcttctgc ctgcgccagc acatttccgc gacctgaaga gagttttttc attgcctggc
    16981 ggtagttatc ctgcgctttg tcgagacttt gaccaatcgc ggacatgtca tcgatgaaca
    17041 aacgcatctt gtcgtacagc ttgctggcac gatcggcgat ttgctgggcg ttgcggcttt
    17101 gatgctcata acgccacagg ttggcaatag tacgcaacgc caccagcagc gtagtcgggc
    17161 taaccagcat gatattgttt ttcaacgctt cggtgatcag ctccggctgg cggtcaagcg
    17221 ccagtaaaaa agcgggttca acgggaataa acatcagcac gtaatccaga gttcgcagcc
    17281 ccggcagctg ttgataatct ttgcgtccca gcaaacggat atggttacgc accgacgcga
    17341 tatgttcctg tagcgcgctt tcgcgggtgt agtcgtcttc ggcgttaaaa tagcgttcat
    17401 aggcgaccag cgtcattttg gcgtcgatca ccacatcttt tccctgcggc agccgcacga
    17461 tgacatccgg ctgcatccgc gagcgggcgt cattttcgat gctgacctgg gtttcatatt
    17521 catacccttc acgcagaccg gaagcctcca gcacccgcgt caatactacc tcgccccagt
    17581 tgccctgggt tttattgtcg cctttcagcg cgcgcgtcag gttgatcgct tcctgggcca
    17641 tttgcgcgtt gagttgctgg agattgcgaa tttcgtgggt cagggtatgg cgttcttgtg
    17701 cttctttacc gaagctgtcc tgaacctgac ggcggaaacc gtccagttgt tcacgtagcg
    17761 gcgacaacag gctgttcaga ctctgacggt tttgctcatc aacccggcga ttgctgtgct
    17821 caaaaatacg gttggcgagg ttttcaaact gctcactgag gcgctgctcg ctgttaatca
    17881 tctggcgaat tttatcgtca gcatgttgct gtgcggcttc catccgcgtg gttacttcac
    17941 gcagatcggc ctccagagag gtgttaatac tttgcaggct gcgcacttcg ttattgagta
    18001 actcgcactc tgcacgccag tgctcgcttt gggtaatttg ttgttttgcc gcgcttaact
    18061 ccgcgaccat ctcttcacgt tcagctaatt gctcggcttt ttgctgcgca tgttgataac
    18121 tggcaaacag ccagccaatt gccacaccca ccaacgcaat aactgcgtaa accatgattg
    18181 agaaatccac aacgcctcct gacgcaacaa cttacccgca caaaatagaa ttgtgctgta
    18241 caaacgtcca gttggaaagg catttcctgc gaggcgctac gcaaaaatac aaaaggccga
    18301 acgcgtcggc cttcagacag gagaagagaa ttacagcaga cgacgcgccg cttccaccac
    18361 gattttcacc gcttggcttt cagtttgttt catcgtctca gcattcggga tctcttgctg
    18421 ggtgcggtta acgataacac ccgctaccat accggcacgc aggccctgac ttgcacacat
    18481 ggtcagcagg gttgcagatt ccatttcata gttcattatg cccatcgcct gccactcttc
    18541 catagaacct ttaaagtgac gaactacgcg accagagtaa gtatcgtaac gttcctgacc
    18601 tgggtagaag gtatcagaag aagctgtcac gccaacgtga gttgtcgcgc caatggattt
    18661 cgcagcttca accagcgcag tcgtacattc gaaatcagcg acagccggga attccagcgg
    18721 tgcgaagtgc aggctcgcgc catccagacg gacagacgcc gtggtaacca ggacatcacc
    18781 cacattaata tgcggctgaa tagcgcccgt tgtaccgata cgcaggaagg tgcgaatgcc
    18841 cagctgtgcc agctcttcaa cagcaataga ggtagacggg ccgccgatac cggtagagca
    18901 gacgataaca ggtttaccat ccagctctgc acgccaggta gtgaattcgc ggtgagatgc
    18961 cagcttaacc ggcttatcca tcagcgcggc gatcttttcc acacgatccg ggtcgccagg
    19021 gacgatggca agcgtagccc cttgtaaatc gtttttagtg aggccgagat gaaaaacatc
    19081 agacttggac atatacaact cctctgtgaa tcggtttagt cagaagaagc aaaaagacac
    19141 tttaccgaag ggtttaacat tttttcgtga tactcatcac catgacgcaa atgcgttgca
    19201 taaatttacc caccaaaagt gattcaaatc acataaatca ctcttcattc tgcaggactg
    19261 cataaaagat gagccgattc aggcaatgtg atttgttgca tgctgactat agttaacatt
    19321 gcgcaacatt tttataaggg tacggagaat accatggcaa caacacaaca atctggattt
    19381 gcacctgctg catcgcctct cgcttcgacc atcgttcaga ccctggacga cgcgattgtg
    19441 gcgggcttca cctctatccc ttcacaaggg gataacatgc ctgcttacca tgccagacca
    19501 aagcaaagcg atggcccact gccagtggtc attgtagtgc aggaaatttt tggcgtgcat
    19561 gaacatatcc gcgacatttg tcgccgtctg gcgctggagg ggtatctggc tatcgcacct
    19621 gaactttact tccgcgaagg cgatccgaat gattttgccg atatccccac gttgcttagc
    19681 ggtctggtag caaaagtgcc tgactcgcag gtgctggccg atctcgatca tgccgccagt
    19741 tgggcttccc gcaacggcgg cgatgttcat cgtttaatga tcaccggatt ctgctggggt
    19801 ggacgtatca cctggctgta tgccgcgcat aatccacagc taaaagctgc agtggcgtgg
    19861 tacggcaaac tgacgggcga caagtcgctg aattcaccga aacaacctgt tgatatcgca
    19921 accgatctta acgcgccgat tctcggctta tatggcggtc aggataacag cattccgcag
    19981 gagagcgttg aaaccatgcg ccaggcgctg cgggctgcta atgcgaaagc agagattatc
    20041 gtgtacccgg atgccgggca tgcattcaac gctgattatc gcccgagcta tcatgccgaa
    20101 tctgcgaaag atggctggca gcgtatgctg gaatggttta agcagtatgg agagaagcgg
    20161 agttagtttc ctcttagtct atatttgaga tggtgagtaa acgagtgcgt tttgctgcca
    20221 tctctgtccc ccaaaaaata atcagtatga attgattgga gattacgact ccgctatttg
    20281 accttaagaa agtcggcaag tcatagtcat aaatgagtct cttcaatgga agacacctta
    20341 gagatttacc atcaactccc attcacaaac aaacattaat tattatcaac acattcacat
    20401 taattataaa tcatctcaga ccttcctgat aaaaagatat taaagtgaaa cattttgttt
    20461 ttcacatttt agttcatcac ttttaggcgt aaataaaggt aaaaaaaaca acaatactat
    20521 ttctctttca caaaagaatt aacaaaaggt ttttcaagga tgagtgatat tatttacctg
    20581 accataaccg gagaacaaca aggatgtatt tcctctcgtt gtggaacgag cgcatcaata
    20641 ggtaatcgct ggcagattgg ccatgatgat gaaatcttcg cgttctcgct ctctaatagt
    20701 attacgaata caggcaaagg gtcataattg catggcctta gtttctgtaa attgattgac
    20761 aaaagctccc cgttacttat taacgctatt aacaataacg agcagttgtt catggagttt
    20821 gacttctatc ggatcaatag gtttggtcga tgggagaaat attacaacat tcaactccgc
    20881 ggcgcgctct tatcagcaat caatcacctg tttactgaga acaatctgga tacagaagcc
    20941 atcactgtca gttattaata tatactctcc aggcatctga ttgctaatac tgaatttagt
    21001 tattgggcat ttcccgataa ctacaaccgt ctgtttatac cacggccaaa aactaaagct
    21061 ggtaatggtt tcaaaacact taatagtaaa gcggttggca gactgcttgc tgctgtcggc
    21121 atatacaatg ggaatatcga aggattcagg gaaacagcag acaagttggg gggagatgca
    21181 cctgctggtt atgatcaatt tatggataac aaagggttac tcatcgctgg tgcttcaata
    21241 gttgctggag cagttctggg gagaatgaga ctccctgatt tggaatcact tgagcatttc
    21301 ggagccagag gagcagtatc aggaagacca tttaatcccg agcttgccgg tggtccaata
    21361 gaaaacctga cgacagatgg cgtaacaatc aacagggaag gtatcgctat tgttgaaaaa
    21421 catatagcca gatttggtca tgatcctgta aatgaagtga tgatcaatag actgaaagac
    21481 attgagaaag gaaaaatccc cccagaacaa gtagatctta acttctatac tcatgagtgt
    21541 cgggaatatc agcgttactg taatcttggt tgggaaacag gacaacctga tggtgacgca
    21601 ggctatgcac tctggaatca tactggattt gcccctatat ttccagacat ctgttatcac
    21661 ttaacccatt acaagcccgc tgcc
//
LOCUS       DAESHI010000069        21643 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.069, whole genome shotgun sequence.
ACCESSION   DAESHI010000069 DAESHI010000000
VERSION     DAESHI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.069"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="KW011_002661"
     CDS             complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="KW011_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HBH7660962.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1345..2997
                     /gene="ushA"
                     /locus_tag="KW011_002662"
     CDS             1345..2997
                     /gene="ushA"
                     /locus_tag="KW011_002662"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HBH7660963.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="KW011_002663"
     CDS             complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="KW011_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HBH7660964.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3717..4511)
                     /locus_tag="KW011_002664"
     CDS             complement(3717..4511)
                     /locus_tag="KW011_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HBH7660965.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4649..4990
                     /locus_tag="KW011_002665"
     CDS             4649..4990
                     /locus_tag="KW011_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBH7660966.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5205..7709)
                     /gene="copA"
                     /locus_tag="KW011_002666"
     CDS             complement(5205..7709)
                     /gene="copA"
                     /locus_tag="KW011_002666"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HBH7660967.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7971..8903
                     /gene="glsA"
                     /locus_tag="KW011_002667"
     CDS             7971..8903
                     /gene="glsA"
                     /locus_tag="KW011_002667"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBH7660968.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8906..10198
                     /locus_tag="KW011_002668"
     CDS             8906..10198
                     /locus_tag="KW011_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH7660969.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10323..10730
                     /gene="cueR"
                     /locus_tag="KW011_002669"
     CDS             10323..10730
                     /gene="cueR"
                     /locus_tag="KW011_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HBH7660970.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10731..11189)
                     /locus_tag="KW011_002670"
     CDS             complement(10731..11189)
                     /locus_tag="KW011_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBH7660971.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11186..12103)
                     /locus_tag="KW011_002671"
     CDS             complement(11186..12103)
                     /locus_tag="KW011_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBH7660972.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12249..12926
                     /gene="fetA"
                     /locus_tag="KW011_002672"
     CDS             12249..12926
                     /gene="fetA"
                     /locus_tag="KW011_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HBH7660973.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12913..13692
                     /gene="fetB"
                     /locus_tag="KW011_002673"
     CDS             12913..13692
                     /gene="fetB"
                     /locus_tag="KW011_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HBH7660974.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="KW011_002674"
     CDS             complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="KW011_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HBH7660975.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="KW011_002675"
     CDS             complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="KW011_002675"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBH7660976.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="KW011_002676"
     CDS             complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="KW011_002676"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HBH7660977.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16063..16749
                     /locus_tag="KW011_002677"
     CDS             16063..16749
                     /locus_tag="KW011_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH7660978.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16746..19160
                     /locus_tag="KW011_002678"
     CDS             16746..19160
                     /locus_tag="KW011_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH7660979.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="KW011_002679"
     CDS             complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="KW011_002679"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBH7660980.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20557..21483)
                     /gene="allS"
                     /locus_tag="KW011_002680"
                     /pseudo
     CDS             complement(20557..21483)
                     /gene="allS"
                     /locus_tag="KW011_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         5013 a         5685 c         5800 g         5145 t
ORIGIN      
        1 tgatcggcga taagccccag aactgccgct cccagacctc ccatgccaaa agcaaaaccg
       61 aaaaagagtc cagaaaccat accgatacgt cccggaagta gctcctgagc gtagactaga
      121 atggcagaga atgccgaagc gaggataaat ccaataatca ccgttaaaac ccccgtccag
      181 tgcaggctgg cgtagggtaa aatcagcgta aacggcgcaa cgccgaggat agagccccaa
      241 atcacatatt tccgtccaat tttatcccct acaggcccgc cgatcaccgt acctgccgca
      301 acggcaaaca ggaaggcaaa cagatgaagc tgggcattct ggatagataa tccgaatttt
      361 tgcatcagat aaaaggtgta atagctgctg atgctcgcca tatagaaata tttcgagaaa
      421 atgaggatta acagaatgct gactgccagt accactttgt tgcgcggcag tggattgatt
      481 atcgtcgctt tgggttttcc tttattcatt cggtgctgtg ccgagtacca acggctaatt
      541 tgcgccaaca ccacgatcgc cagcagtgcc gcaagcacaa accaggcaac gttgcctttg
      601 ccataaggcg cgataatcac cgccgccagc aagggtccca gggaactgcc aaagttgccg
      661 ccgacctgaa agatagattg cgccaggcca tgccgcccgc cggaagccat acgggctacg
      721 cgagaagatt ccggatgaaa gaccgatgaa ccggtaccga ccagcgccgc cgccagcaga
      781 actgcgccaa aactgcccgc cagcgcaagc agcaccagac cacttaaggt aaagcacatg
      841 ccaattggca acgaccacgg catcggatat ttatcggtcc agtagccgac cactggttgc
      901 agtagcgaag aggcgagctg gaaggtgagg gttatcatgc caatctgcat aaatgtcaga
      961 gaaaattctg actgaagcag cggataaatc gccagaatca gcgattggat catgtcgttc
     1021 agcagatgtg agaggctgat agcacctaaa ataccaaacg atgttcgggc cttggtcgtt
     1081 gacgcagccg cgcccgccac aggctggggt tgttcactca ttgccatagg aaagtcactt
     1141 tttcagggtt gcgatgtaaa gaatgatctt atttgtgatt attacccgac taacatacct
     1201 gtatgcgtcg tctgaaggaa gtctcaacgc cgaatacaga atttctaatc tggatgcaga
     1261 tttatcttca ccggacgcag acttgtctat gatgtcgcgt catactattt ttcaacacgt
     1321 tgaaatcagg tcagggagag aagtatgaaa ttattgcagc ggggcgtggc gttagcgctg
     1381 ttaaccacat ttacactggc gagtgaaact gctctggcgt atgagcagga taaaacctac
     1441 aaaattacag ttctgcatac caatgatcat catgggcatt tttggcgcaa tgaatatggc
     1501 gaatatggtc tggcggcgca aaaaacgctg gtggatggta tccgcaaaga ggttgcggct
     1561 gaaggcggta gcgtgctgct actttccggt ggcgacatta acactggcgt gcccgagtct
     1621 gacttacagg atgccgaacc tgattttcgc ggtatgaatc tggtgggcta tgacgcgatg
     1681 gcgatcggta atcatgaatt tgataatccg ctcaccgtat tacgccagca ggaaaagtgg
     1741 gccaagttcc cgttgctttc cgcgaatatc taccagaaaa gtactggcga gcgcctgttt
     1801 aaaccgtggg cgctgtttaa gcgtcaggat ctgaaaattg ccgttattgg gctgacaacc
     1861 gatgacacag caaaaattgg taacccggaa tacttcactg atatcgaatt tcgtaagccc
     1921 gccgatgaag cgaagctggt gattcaggag ctgcaacaga cagaaaagcc agacattatt
     1981 atcgcggcga cccatatggg gcattacgat aatggtgagc acggctctaa cgcaccgggc
     2041 gatgtggaga tggcacgcgc gctgcctgcc ggatcgctgg cgatgatcgt cggtggtcac
     2101 tcgcaagatc cggtctgcat ggcggcagaa aacaaaaaac aggtcgatta cgtgccgggt
     2161 acgccatgca aaccagatca acaaaacggc atctggattg tgcaggcgca tgagtggggc
     2221 aaatacgtgg gacgggctga ttttgagttt cgtaatggcg aaatgaaaat ggttaactac
     2281 cagctgattc cggtgaacct gaagaagaaa gtgacctggg aagacgggaa aagcgagcgc
     2341 gtgctgtaca cccctgaaat cgctgaaaac cagcaaatga tctcgctgtt atcgccgttc
     2401 cagaacaaag gcaaagcgca gttggaagtg aaaataggcg aaaccaatgg tcgtctggaa
     2461 ggcgatcgtg acaaagtgcg ttttgtacag accaatatgg ggcggttgat tctggcagcc
     2521 caaatggatc gcactggtgc cgactttgcg gtgatgagtg gaggaggaat tcgtgattct
     2581 atcgaagcag gcgatatcag ctataaaaac gtactgaaag tgcagccatt cggcaatgtg
     2641 gtggtgtatg ccgacatgat cggtaaagag gtgattgatt acctgaccgc cgtcgcacaa
     2701 atgaagccag attcaggtgc ctacccgcaa tttgccaacg ttagctttgt ggcgaaagac
     2761 ggcaaactca acgacctgaa aatcaaaggc gaaccggtcg atccggcgaa aacttaccgt
     2821 atggcgacat taaacttcaa tgccaccggc ggtgatggct atccgcgcct tgataacaaa
     2881 ccgggctatg tgaataccgg ctttattgat gccgaagtgc tgaaagcgta tatccagaaa
     2941 agctcgccgc tggatgtgag tgtttatgaa ccgaaaggtg aggtgagctg gcagtaatcc
     3001 gaaagtgccg gatatttgca tccggcacaa tgcttaatcg cggcgggcga tatcagcaaa
     3061 tttggcatcg aggatcttcg ccagatcgcc tgccgccagt tcgatatcca gtccgcgctt
     3121 gccgccagaa acataaatag tggcaaattc ttgtgcgggg gcgtcgataa tcgtcggcag
     3181 acgttttttc tgccccagtg ggctaatccc cccaaccagg tatcccgtcg aacgctgcgc
     3241 ggccatcgga tcggccatct caactttctt ggcacccagc gcttttgcta cttttttaag
     3301 atccagttga cctgcgaccg gcgtaacggc cactgccaga tgtttcatat caccgttcac
     3361 tgccaccagt agcgttttgt agacttgatc cggattcaaa cctaattttt tgacgacttc
     3421 atcgccaaaa ttggtttcag ccggatcgtg ctcgtaggta tggatttgaa acgaaatctt
     3481 gtttttttcg agtaatttaa ctgcgggtgt catggtaatt ttcctgcctt aaacaaaaaa
     3541 acgaagcaag catacgcctg atggcggtcc aaaaaaaaga gtcatcttgc ctaagagtat
     3601 tggcaggatg gtgagattga gcgacaatcg agttacaccg tcgcttaaag tgacggcata
     3661 ataataaaaa aatgaaattc ctctttgacg ggccaatagc gatattggcc atttttttag
     3721 cgcaacattt gcggcaaatt cccttctcca tacaggtgta gtgcaccgac cgcgaccaca
     3781 tatcgccccg gcggcatggc gcgtaattta tcccgccagg cgagatttcg ctgatgcatc
     3841 agcacatcgt acagcgactg actgaacgta ttgggcagcg ttatatcatt attttgcgga
     3901 ggtgcattca gccaccagct catcatttgt tgcagcaacc gtgcgttggt atgccagtgg
     3961 gtcagcgtat cgtccagcag cgccagtcct ttgtcaggga gctggagcaa catggtaatc
     4021 tggttttcag ccccttccag ttcaatcacg ggtttatgtt gttgcttcgc cgcctgcaat
     4081 aactggtaat cgataccgta ttctgcccgc agccccagtt tttgtgcctg cgtcgcctga
     4141 agaaccatcg cgatttgcca cagcggttga gtagaaaaga gcgacggaga aatgcccatc
     4201 tcctggctaa tgtgctgcag attttggagt tgctcctcgt taatgcgctc ttccagcgcc
     4261 tcgcaagtag gcaaattagc aaaaggcgta tcgctggtgg aaacatccgc ctcgacgatc
     4321 agcgcatcgg cgtttttgag ctttttgagc aaacgggtgg gcaggggagc catatcgtgg
     4381 ctacccatat gaatactgcc aatcagatga aaatggcgat tgccgggaag ggtgatatcg
     4441 atggcaggcc aggtgtaatg attaccgcgc agcgcggccc aaagcgtttt tacccggtac
     4501 aacagatcca tacgacctcc ctttgtgaaa tatcatgcta gcgcgcggtg agggatggcg
     4561 caaatggagt tggggacttg tttaatgtgt ttgtatgatt cagtatgttc ttgcatcgct
     4621 attcacaggg aagcaacagt taaaaaccat gaaacaggca acaagaaaac cgacgacacc
     4681 cggagatatt cttctctatg aatatctgga accgctcgat ttgaaaatca atgagttagc
     4741 agagttgctg catgttcatc gtaatagcgt cagtgcactg atcaataaca atcgtaaact
     4801 caccactgag atggcattcc gtctggcgaa agtttttgat accacagtcg attttaggct
     4861 aaacctccag gcggcagttg atctttggga agttgaaaac aacatgcgca cccaggaaga
     4921 attgggacgg attgaaacag tggctgaata tttggcacgc cgtgaagagc gtgcaaaaaa
     4981 ggtcgcgtaa ggccaaaaca tcgcttcatc tcgtaggccg gataaggcgt tcacgccgca
     5041 tccggcatcc gagcaccatt gcctgatgcg acgcttaccc gtcttatcag gcctacaaac
     5101 ctgtgcctga accgtaggcc ggataaggcg tttacgccgc atccggcagt catgcgtcga
     5161 tgccaaatgc gccgccctaa agcagcgcat ccgcaatgac gtacttattc cttcggttta
     5221 aaccgcagca accggttggc gttactcact acggtaatcg acgagagcgc cattgccgct
     5281 ccggcaacta ccgggttaag cagtgttccc gtaaacggcc acaaaatacc ggcggcgacc
     5341 ggaataccga tgctgttgta gataaaggca ccgagcaggt tctgcttcat gttgcgcagc
     5401 gttgcgcggg aaatcgcgag cgcatccgca acgcccatca ggctatggcg catcagggta
     5461 atcgccgcgg tttcaatggc aacatcactg ccgccaccca tcgcaatgcc gacatccgcc
     5521 tgggccagcg ctggcgcgtc gttaatgccg tcgcccacca ttgccacctg acgtccttca
     5581 ctttgcagac gtttgatcgc ttcggcttta ccgtccggca gcactccggc aatcacttca
     5641 tcaattccag cttctttggc gatcgcattg gcggtggttg ggttatcccc ggtcaacatc
     5701 accagacgat atcccgtttt atgcaggcgt tgcagcgccg ccacgctatc actacgcaac
     5761 ggatcgcgta cagccagcag ggctactgct ttcccgtcaa tcgccagcag cacaggcgtt
     5821 gctccttgcg atgcctgagc agtaatctcc gcttcgatag ctttggtacc aacctgttgc
     5881 tcatttaaca gcgcctgatt gcccagcaat aacgcatgac cttcagcttc accgctcacg
     5941 cccagcccgc gcaatgtgcg gaaaccgttg acctgcggca gctgcatatc acctgcttta
     6001 tcgaggatcg cccgtgccag cggatggctg gaaccttgct ccagtgccgc cgccagacgc
     6061 aatgcctgcg cttcatcaac atcagcaaac gttttcactg cgacaacctg cggcttccct
     6121 tcggtcagcg tcccggtttt atcgaacacg acggtatcga gtgtactggc gcgttgcagc
     6181 gcgtcggcgt cccgcaccag cacgccaaac tcagccgccc gcccgacgcc ggaaataatc
     6241 gacatcggcg tcgccagccc cagcgcacac ggacaggcga taatcagtac cgtggtggca
     6301 atcaccaggg tataaacaat ttgcggtgcc ggaccaaaga agtaccagat tgccgcactg
     6361 accagcgcaa tcaccaccac cacaggtaca aatacggctg agattttatc cgccagctga
     6421 ccgatttctg gcttgctgct ctgggcctgg cgcaccatgc gaatgatccg tgacagcgtg
     6481 gtatggctgc caaccgcact ggcacgaaac agcacactgc cgtcctgtac cactgtcccg
     6541 gcatggacgc tttcgccttc gcctttttgc tgtgggattg gttcgcccgt cagcatcgct
     6601 tcatccagcc atgcttcgcc ctgggtaatc tcgccatcta ccggcacgcg atcgccggtc
     6661 gtcaggcgca gtaacatccc tggctgaact tccgccagcg gcacgctttt ttcaccatct
     6721 gcggtgacca ctcgcgccgt cggtggggtt aaatcgagta atttctccag cgccttagaa
     6781 gaacgctgac gtgcgcgcgc ttccagcata tggccgagat taatcagacc gataatcatc
     6841 gcactggctt catagtaaag atgccgcgct tccatcggga accactgcgg ccacaggttg
     6901 acgctcatcg aatagagcca cgccacaccc gttcccagcg ccaccagcgt atccatcgtc
     6961 gctgcaccgt tcagcaggct tttccatgca ctgcggtaaa aatggccgcc ggcgaaaacc
     7021 atcactgcca gggttatcag gccgataact aaccacaagc tgcggttgtc agcggtgacc
     7081 atcatgttat cgccgatcat cccccagacc attaccggga tacccacagc cagtgcaaca
     7141 attgcctgcc agcggaagcg cttcatcgta gctacggcgg tttcttgctg gcgctcgcgg
     7201 cgtttagcgt catcttcaat cgcttccgca ccgtagcccg ctttttccac cgcctgcact
     7261 aaatcttgtg gggaggcact gcccatcacc agcgcagtac gctccgccag gtttacccgt
     7321 gcctgagtga cgcccggtac gctttgcagc gcattttgta cgcgggtgac acagctggcg
     7381 cagctcatgc cgctcagcag caactgctgg ctgtcatcgt catcggcggt cgctgccgga
     7441 agcgcctcag aaaccgctgt cagtgcttcc gacgggattg atgactccgc cagcggttta
     7501 gcctttgggt ggcttacaga tgcgtcataa cccgcttgtt tgatggtttc gatcagctgt
     7561 tctgcactgg cagtcccggt aacgtgcgct tcagtgatag acacatccgc ctgctcaaca
     7621 tccgggcgct gttcaagact ttctttcacg cgtttaacgc agtgaccgca ggacaggccg
     7681 tccagggtca ggtcgatagt ttgtgacata aaacactcct ttaagacagt tttgactggc
     7741 tgtgataaag gttaaacctt ccagcaaggg gaaggtcaag aaattaataa accaggcggg
     7801 taaaagtccg taaagattaa aaaatcggct cgatttgcat caggattaga catttatttc
     7861 tttgttttcc tgtagttaag ttgcgggtgc taagttaaat caggatgcct gaaaatcggc
     7921 accggggtga ggaattacct cccgcatcta taaaaaggag ttaacaaaag atgttagatg
     7981 caaacaaatt acagcaggca gtggatcagg cttacaccca atttcactca cttaacggcg
     8041 gacaaaatgc cgattacatt ccctttctgg cgaatgtacc aggtcaactg gcggcagtgg
     8101 ctatcgtgac ctgcgatggc aacgtctata gtgcgggtga cagtgattac cgctttgcac
     8161 tggaatccat ctctaaagtc tgtacgttag cccttgcgtt agaagatgtc ggcccgcagg
     8221 cggtacagga caaaattggc gctgacccga ccggattgcc ctttaactca gttatcgcct
     8281 tagagttgca tggcggcaaa ccgctgtcgc cactggtaaa tgctggcgct attgccacca
     8341 ccagcctgat taacgctgaa aatgttgaac aacgctggca gcgaatttta catatccaac
     8401 agcaactggc tggtgagcag gtagcgctct ctgacgaagt caaccagtcg gaacaaacaa
     8461 ccaacttcca taaccgggcc attgcctggc tgctgtactc cgccggatat ctctattgtg
     8521 atgcaatgga agcctgtgac gtgtacaccc gtcagtgctc tacgctcatc aatactgttg
     8581 aactggcaac gcttggcgcg acgctggcgg cgggtggtgt gaatccgttg acgcataaac
     8641 gcgttcttca ggccgacaac gtgccgtaca ttctggccga aatgatgatg gaagggctgt
     8701 atggtcgctc cggtgactgg gcgtatcgcg ttggtttacc gggcaaaagc ggtgtaggtg
     8761 gcggtattct ggcggtcgtc cctggcgtga tgggaattgc cgcgttctca ccaccgctgg
     8821 acgaagaagg caacagtgtt cgcggtcaaa aaatggtggc atcggtcgct aagcaactcg
     8881 gctataacgt gtttaagggc tgatcatgat gaacacggaa ggtaataacg gtaacaaacc
     8941 cctcggtcta tggaacgtcg tttccatcgg tattggggca atggtggggg cggggatctt
     9001 cgcgctgctg gggcaggctg cgttgctaat ggaagcctcg acctgggtcg cctttgcttt
     9061 tggcggtatt gtggcgatgt tttccggtta tgcctatgcg cgtctggggg cgagctatcc
     9121 cagcaatggc ggtattatcg acttttttcg tcgcggatta ggcaacggcg tcttttcgct
     9181 agcgctctcg ttgctgtacc tgttgacgct ggcggtgagc atcgccatgg ttgcccgtgc
     9241 ttttggcgct tatgccgtgc agtttttgca tgaaggcagc caggaggagc accttatttt
     9301 gctctacgcg ttggggatca ttgcggtgat gacgcttttc aactccttaa gcaaccatgc
     9361 ggtagggcgg ctggaagtga tcctcgtcgg cattaaaatg atgatcctgt tattgctgat
     9421 tattgccggt gtctggtcgc tgcaaccggc gcatatttcc gtctctgcgc cccccagctc
     9481 cggtgcgttc ttctcctgta ttgggataac cttccttgcc tatgcgggct ttggcatgat
     9541 ggcgaacgcg gcggataaag tgaaagatcc gcaggtcatt atgccacggg cgtttctggt
     9601 ggcgattggc gttaccacgt tgctttatat ctcgctggcg ctggttttgc ttagcgatgt
     9661 atcggcatta gagttagaaa aatatgctga taccgccgta gcgcaggctg cttctccgct
     9721 gctcgggcat gtgggttatg tgatcgtcgt catcggcgct ttactggcga cggcttcagc
     9781 cattaacgcg aacctgttcg ccgtgtttaa catcatggac aacatgggca gcgaacgcga
     9841 actgccgaag ctaatgaata aatccctgtg gcggcagagt acctggggca acattattgt
     9901 cgtggtgttg attatgctga tgacggcggc gctaaattta ggctcactcg ccagtgttgc
     9961 cagcgccacc tttttgattt gctacctggc ggtgtttgtg gtggcgatcc gcctgcgtca
    10021 tgatattcac gcctcgttgc cgattcttat cgttggtacg ttggtgatgt tgttggtgat
    10081 cgttggcttt atctacagtc tgtggtccca gggtagccgt gcgttgatat ggattattgg
    10141 ctcactctta ctcagcctta ttgtggcaat ggtcatgaag cgcaataaaa ccgtataaca
    10201 tctctctgtg cgcagtactt cctgtattat tgtggtggcg gtcgatattc gcactggcaa
    10261 aaaaacgtgc ttgaatatct gttgaaaccc tttaacaaag cacaggaggc gttgcgcgaa
    10321 cgatgaacat cagcgatgta gcaaaaatta ccggcctgac cagcaaagcc attcgcttct
    10381 atgaagagaa ggggctggtg acgccgccga tgcgcagcga aaacggttat cgcacctaca
    10441 cgcagcagca tctcaacgaa ctgaccttac tgcgccaggc acggcaggtg ggctttaacc
    10501 tggaagagag cggcgagctg gtgaatctgt ttaacgaccc gcagcggcac agcgccgacg
    10561 tcaaacggcg cacgctggag aaggtggcgg agatcgaacg acacattgag gagctgcaat
    10621 ccatgcgcga ccagctgctg gcactggcga atgcctgccc tggcgatgac agcgccgact
    10681 gcccgattat cgaaaatctc tccggctgct gtcatcatcg ggcagggtga ttaagacgag
    10741 acggctcgga tatgtagggt tatcccttct atcgcaatga cttcaacatg cgtacctgca
    10801 ccgagatcct cgctggcgct gacaggccat gaactgtcac cgacgcgcat atgaccgcgc
    10861 ccgttgacca gcggagattc cagcacaaaa cgtcggccaa tcagctgctg cccgcgctgg
    10921 tttaaatgac tatcgctgtg cttttgttcg cgcacccgcc gcgacaacca tttccaccac
    10981 agccaggcgg cgagcagcgt caggatggca aacattaccc cttgccactc ccaacccagc
    11041 ggcaccagcc agaccaccag gccggtaatc accgctgcca cgccgctcca caacaaataa
    11101 ccatttccgc ccagcatctc ggctgccagc agcaaaccgc cgagactgag ccagaaaata
    11161 tgtggatgaa cgaccattaa ctccatcatg gctgagtccg tttgttggcg ctgtctttca
    11221 ccagctcggc aatcccggca atcgacccca tcaggctgct ggcctctaat ggcatcatca
    11281 ctactttgct gttactggag gtaccgatct gctgtaacgc ttcggtgtat ttctgcgcta
    11341 cgaagtagtt caccgcctga atatcaccgg aggcgatagc ttcagacacc attttggtgg
    11401 cgcgggcttc tgcttcggcg gaacgttcac gcgcttcagc ctgtaaaaac gccgactgac
    11461 gttcgccttc cgctttcagg atttgcgact gtttttcacc ttcggctttg aggatttccg
    11521 cctgacggat cccttccgct tcaagaatgt aagcgcgttt ggtacgttcc gctttcatct
    11581 gcgcgttcat tgaagagata agctctgccg gtgggcgcac gtcgcgaatt tcaatacggg
    11641 tgactttaat cccccacggg ttggtggctt catcgacaat acgcagcagg cgtgagttga
    11701 tgctgtcgcg ctgagagagc atttcgtcaa gttccattga acccaacacg gtacggatgt
    11761 tagtcatggt caggttgatg atcgccagct ccagattgct gacttcataa gccgcgcgcg
    11821 gcgcgtcaat cacctgaata aaacagactg cgtcgatggt aacgttggcg ttatctttcg
    11881 agataacttc ctgggaaggg atatcgagca cttgctccat catattgatc ttgcgaccaa
    11941 tgcgatccat aaacggcacc accagactga gccccggctg taacgttttg gtatagcgac
    12001 caaaacgttc taccgtccat tgatagccct gcggcacgat tttgacgccc gcgccgacaa
    12061 tgaccagcgc gacaaaaatg agaatcggga taaagataag catcggaaaa acctcctgtt
    12121 gtaccgtcca taatcagcaa aattgctgct tgattaaaca aattatacct gattactgaa
    12181 agagagttcc cccttattcc tgcgaaggat aaactgtttt tagtaaaaat cagaaaaagg
    12241 gaacagcgat gcaggaaaat agtcctttgc ttcagctaca aaacgtagga tatctggcgg
    12301 gtgatgcgaa gattcttaat aacatcaatt tttcgctgcg tgctggcgaa tttaagttaa
    12361 ttaccggtcc ttctggttgt ggcaaaagta cgctgctaaa aatagttgct tcattgatca
    12421 gcccaaccag cggaacgtta ctgtttgaag gtgaggatgt cagcacacta aagccagaaa
    12481 tctaccgcca acaagtctct tactgcgccc agacaccgac gctgtttggc gatacggtat
    12541 acgataatct gatctttccc tggcagatcc gtaaccggca gcctgaccca gccatttttc
    12601 tcgattttct cgaacgcttc gccttgccgg acagcatttt gacgaaaaat atcgccgagc
    12661 tatctggtgg tgaaaaacaa cgcatctcac tgattcgtaa cctgcaattt atgccgaagg
    12721 ttttattgct ggatgaaatc accagtgcgc tggatgaaag taataaacat aacgttaatg
    12781 agatgatcca tcgttatgtg cgcgagcaaa atattgccgt gctgtgggtg acacacgata
    12841 aagacgaaat taatcatgcg gataaagtga ttacactgca accgcatgcc ggagaaatgc
    12901 aggaagcacg ctatgaactc gcataatatt actaacgaat cattagcact ggcattaatg
    12961 ctggtggtgg tggcaatctt aattagccat aaagaaaaac tggcgctgga gaaagatatt
    13021 ctctggagcg tcggacgagc gataattcag ctgattattg tcggctatgt gctgaagtat
    13081 attttcagcg tggatgatgc cagcctgaca ttattgatgg tgttatttat ctgctttaat
    13141 gcggcgtgga acgcgcaaaa acgcagtaaa tatattgcta aagcttttat ctcatcgttt
    13201 attgccatta cggtcggggc gggaattacc ctggcggtgc tgattctctc agggtcgatt
    13261 gaatttatcc cgatgcaggt gatccctatc gccgggatga ttgccggtaa cgccatggta
    13321 gcggtggggt tgtgttacaa caatttaggg caacgggtca ttagcgaaca gcaacagatt
    13381 caggagaaac tgagtcttgg tgcgacgccg aagcaggctt cagcgatatt gattcgcgac
    13441 agtattcgcg cggctttaat tccgacggtc gattcagcaa aaacggttgg cttagtgagt
    13501 ttaccaggaa tgatgtccgg gctgatattt gccgggattg atccggtgaa ggcgattaaa
    13561 tatcagatta tggtgacctt tatgctgctc tcaaccgcca gcttgtcgac cattattgcc
    13621 tgctatttaa cctatcgtaa gttttataat tcgcgccacc agttggtggt gacgcaattg
    13681 aagaagaaat gatgcgatgc cggatgcgca gcatcgcatc cggcgttgtg gttcatgtgc
    13741 cgatcaacgg aatatcaata caacaatgca tacagctggc ggcgatactt cgacgccagt
    13801 gcatcacccg tacccagcgc agcgaggatc tcctggaacg ttttacgcgt ctgaccgtct
    13861 gcggcggtga gatctttacg cagatgcccg aacagcaact ccagcgcctc ttcattgcgc
    13921 ccaacctgat gcagttgcag cgccagttgc gtcgccagtg cggcatcttc tggattctcc
    13981 gccacctgct gttgcaactg ttgaatttcc ggcgtatcag ccgcctgctt cagcagttcg
    14041 atttgcgcca ccatcccctg gtagcgggtg tcctgatcct gtaacggaat ggttttcagc
    14101 accgcttctg catcttcaga acggttcagc gcaatcagcg tttctgccaa cagcaggccg
    14161 atctccccgt tctgattcga caactgccag gcgtctttca gcaatggcag ggcatcggtg
    14221 aaattgcctt cctgcatcag ttgcatcgcc tgctgcgctt tcagctcttc ttcgcgcggc
    14281 agcactttat ccagcagggc gcggatcgcc tcttccggtt gcggcccctg gaagccatct
    14341 accggttgcc cgttctggaa cagatacacc gtcggaatcg cgcgcagacc aaactgggcg
    14401 gcaatcatct gctccgcatc gcagtccagc ttcgccagaa taaattgccc gttgtactgc
    14461 gccgcaaggc tttccagaat tggggttaac tgcaaacagt gctggctacg ttcagaccaa
    14521 aaatagaaca gcaccggagt ggtcatcgac tgttcaagaa cctgttgcag gttagattcg
    14581 ttaatgttga caatattttc tacggacatg gagtcgctct ctgttgtcga tattttcttt
    14641 gacatggggg cttaagcgcg cgcttcaact cacccctgca atattttgtc catcacgcgc
    14701 cccggcagca ggcgcttaag caccattact gcccaagtca ccagcgttac cggatagcgc
    14761 atcttcggct tctcgctaat aaaagcatgg cgtactttgt ccaccaccgc ttccggtccc
    14821 aacgtaaagc gggcggcgat gccgggattt tcgactggtt tatcactttg cgtctggttg
    14881 acgttgtcgg tgaagcgagt acgaatggga ccgggttcga tcaggctgac tttaattccg
    14941 ctgtggcgta gctccatgcg cagcgcgtct gaccatgcct ccagcgcata tttactggcc
    15001 gcgtaagcgc cacgacccgg tgtggagatt aatcccatca ccgatgatgt catcacaata
    15061 cgcccttcac cgtgcggtaa catcgcgggt aacaggcgca tggtgagctg gtgtgcgccg
    15121 aaaaagttgg cggaaaactg ctgttccatc tgcgcgcggc tgatggtgga aagggggcca
    15181 tacatgccga atccggcatt gttaaagatc ccatacagac aattatcggt cagggcgatc
    15241 acttcgtcgg ctgcgcgatc aacactttct ggcgaatcca gatcgatcaa cacgccggta
    15301 aatcccatgc tgttcatacg ctcaacatca tccggtttcc ggcaacctgc cagcacatga
    15361 aaaccctggc gttttaattc gagcgcgctt tccaggccaa ttccactgga acatccggta
    15421 attaagaccg atttttgcat aactttacct gtcaggatct ccgttgcttt atgagtcatg
    15481 atttactaaa ggctgcaact gcttcgccat ccagtcggca ataaacggct gggcgtcgcg
    15541 gttgggatga ataccgtcat cctgcatcca ttgtggcttg aggtagacct cttccataaa
    15601 aaagggcagc agcggaacat caaactcttt ggcgagtttg gggtaaatgg cgctaaaggc
    15661 ttcattataa cggcgaccat agtttgcagg cagacgtatt tgcattaaca atggttcagc
    15721 gttggcggct ttgacatcct gcaaaatctg gcgcagcgtt tgctcggttt gctgtggctg
    15781 aaaaccacgc aaaccgtcat tgccgcccag ttcaaccagc acccaacgcg gctgatgctg
    15841 tttcagcaga gccggaaggc gcgccagtcc ttgttgcgag gtgtcgccgc tgatgctggc
    15901 attaactacc gacgttttac tctgccactt atcattcaac aaggcaggcc aggccgcgct
    15961 ggcagacatt cgatacccgg cgctcaggct atcacccaga atcaataacg tgtccgctgc
    16021 ggcggcacgg aaggttaaca gggccaggaa caggaagggc aaatgccagc ggaaaacatt
    16081 gttgaagttc atcatcttaa gaagtccgtc ggtcaggggg agcatgaact ctccatcctc
    16141 accggagttg agctggttgt caaacgtggc gagaccatcg cactggtggg cgagtcggga
    16201 tcgggtaagt caaccttgct ggcgatcctc gccgggcttg atgacggcag cagtggcgaa
    16261 gtgagtctgg tgggacaacc gctacataat atggacgaag aagcgcgggc aaagttgcgc
    16321 gcgaagcacg tcggctttgt ttttcagtca tttatgttaa ttcctaccct taacgcgctg
    16381 gaaaacgtcg agcttccggc tctgctgcgc ggtgagagta gcgcggaaag tcgtaacggg
    16441 gcgaaagcgt tgctcgaaca gttagggctg ggtaaacgtc tggatcatct tccggcacag
    16501 ctttccggcg gtgaacagca acgagtggcg ctggcacgag cctttaatgg tcgacctgat
    16561 gtgctgtttg ccgacgaacc caccggcaac cttgaccgcc agacgggcga taaaattgcc
    16621 gacctgctgt tttccctcaa ccgtgaacat ggcaccacgt tgattatggt gacccacgac
    16681 ctgcaactgg cggcacgctg cgaccgctgc ttacggctgg tgaacgggca gttgcaggag
    16741 gaagcatgat tgcacgttgg ttctggcgcg aatggcgttc gccgtcgcta ttaattgtct
    16801 ggctggcgct aagcctggcg gtggcctgcg tgctggcgct gggcaatatc agcgatcgca
    16861 tggagaaggg cttaagccag caaagccgtg agtttatggc gggcgatcgg gcgttgcgca
    16921 gttcacgcga agtgccgcaa gcgtggctgg aggaagcgca aaagcgcggc ctgaaagtcg
    16981 gcaagcagct gactttcgcc acaatgacct ttgcaggcga cacaccgcag ctggcgaacg
    17041 tcaaagcggt ggatgatatc tacccgatgt atggcgatct gcaaactaat ccccctggcc
    17101 tgaaaccgca ggcgggcagc gtattgctgg ccccacgcct gatggcactg cttaacctga
    17161 aaacgggcga caccattgac gtgggcgatg ccaccttgcg gattgccgga gaagtgattc
    17221 aggaaccgga ttccggtttt aaccccttcc agatggctcc gcgtctgatg atgaatctgg
    17281 cggatgtcga taaaaccgga gccgtgcagc cggggagtcg ggtcacctgg cgttataaat
    17341 tcggcggcaa cgagaaccag ctcgacggct atgataaatg gttgttacct cagcttaaac
    17401 ccgaacaacg ctggtacggt ctggaacagg acgaaggcgc gctggggcga tcgatggaac
    17461 gctcgcaaca gttcctgctg ctttcggcgc ttctgacctt gctgctggca gtggcagcgg
    17521 tggcggtagc gatgaatcat tactgtcgca gtcgctacga tctggtggcg atcctcaaaa
    17581 cgctgggggc agggcgagcg caactgcgta agctaatcgt cggtcagtgg ttgatggtgc
    17641 tgacgctttc agccgttacc ggtggggcca taggcctgtt gttcgaaaac gtgttgatgg
    17701 tgctgctcaa gcccgttctg cctgctgcac taccgccagc cagcctctgg ccgtggctgt
    17761 gggcgcttgg caccatgacg gtcatctcgc tgctggtggg gctacgacca taccgcttgt
    17821 tgctggcaac gcagccttta cgcgtattac gtaatgatgt ggtagcgaac gtctggccgc
    17881 tgaagtttta tctgccgatt gtcagtgtgg tggttgtgct gctgctcgcc ggattaatgg
    17941 gtggcagcat gctgctttgg gcggtgctgg cgggcgcggt agtactggct ttgctgtgcg
    18001 gtgtgctggg ctggatgcag ctgaatgtac ttcgccgcat gacgctgaaa tcgctacctc
    18061 tgcgcctggc ggttagccgc ctgttacgtc agccgtggtc aacgttaagc cagctttcgg
    18121 cattttcgct ctcctttatg ctgctggcgc tgttgctggt attgcgtggc gatctgctcg
    18181 accgctggca gcagcagcta ccgccagaaa gcccgaacta ctttttaatt aacatcgcca
    18241 cagaacaggt agcaccgcta aaagcgttcc tcgcggaaca tcatatagtc ccggaatcat
    18301 tttatccggt agtgcgggcg cggctgacgg cgattaacga taagccgaca gaaggtaatg
    18361 aagatgaggc gcttaatcgc gaactcaatc ttacctggca aaatacgcgg cccgatcata
    18421 atccgattgt cgccggtaac tggccgccaa aagccgatga agtgtcgatg gaagaggggc
    18481 tggcgaaacg cttaaacgtt gccctcggtg ataccgtgac ttttatgggc gatacccagg
    18541 agttccgcgc taaagtgacc agcctgcgca aagtggactg ggaaagtctg cggcctaatt
    18601 tctattttat tttcccggaa ggggcattag acgggcaacc gcagagctgg cttaccagtt
    18661 tccgctggga gaatggcaac ggcatgttga cgcaactcaa ccgccagttc ccgaccatta
    18721 gcctgttaga tattggcgcg attttaaaac aggtcggtca ggtgctggag caggtaagtc
    18781 gggcgctgga ggtgatggtg gtgctggtca ccgcctgcgg tatgttgctg ttgctggcac
    18841 aggtgcaggt gggaatgcgt cagcgtcatc aggagctggt ggtgtggcgc acactcggtg
    18901 cggggaaaaa actgctgcgt accacgttgt ggtgtgagtt cgccatgctt gggtttgttt
    18961 ctggcctggt agccgcaatt ggtgcggaaa cggcgctggc agtgttgcag gcgaaagtgt
    19021 ttgatttccc gtgggagcca gactggcgat tgtggattgt tctgccgtgc agcggtgcac
    19081 tgctgctgtc gcttttcggc ggctggctgg gtgcgcgact ggttaagggt aaggcgctgt
    19141 tcaggcagtt tgcggggtga tgacaggtgt tagattgcct gatgcgctac gcttatcagg
    19201 cctacgaaga gcgtgcaata tattgatttt gcaagattgt gtaggccgga taaggcgttt
    19261 acgccgcatc tggcatgaac agtgtgcatt ttggcaataa atatgaaacc aactgtcatg
    19321 tcggtttttt atatctgtat tctggtgcaa ccattctgtt ctcacgacaa tcgccgtttc
    19381 agaataccgc ccatcactgc gtcttaaccc attccgccac ttccgcccac tcaccgcgaa
    19441 agacaacttt ttccgctttt ttctcaagct gatagcgata catcgggtcg taatattctt
    19501 taagtaacgg caccagccag gccagatgac cgtcggtgct gtcggtggtg agttgcgttg
    19561 tcagcgccgt atccagctgg gcagcgagtt cgttatagcg ctgtaacccc agccgacgct
    19621 taatagccga aagtccgtga tgcaggtatt cgcaatattc ctgccagccc tgttcgtcgc
    19681 cgtacgcgtg ggtaaaatca tgatgcatac gcaagaaata ctcttcgttc aggcgctcaa
    19741 gacggatctc aaacggatct tctaccaccg caatcgccgc ctgagtcatt cgctcgcgca
    19801 ggcattccgg caggtgattc gaaccgatca tccggctttc gtcttccagc acccacaggc
    19861 gcaaatcctg acgggcgtcg gtttttagca tttcggcagc cagcaggttt tcaaaactcg
    19921 cctggctaag ttgtggttgt aaggtgcgac caaacgccga accgcgatga cgcgctaacc
    19981 cttccagatc aacaccgttc ggctgttgct gcactaacag cgttttaccg ctgccggtac
    20041 aaccgccaat cagcactatc ggtttttgtg ccagttcaat agtcgcctga atcgcggctt
    20101 ggcgcagtgc cttataaccg ccttccacca gcggataatc aatccccgct gcatacaacc
    20161 agctttgcac aatatgtgag cgctgaccgc cacgggcgca gcagagaata ccttgcggat
    20221 tttgcaggca cgctgcccgc caggcgtcca tgcgctgctg acgaatttca cccgccacca
    20281 gtttatgccc cagcgccagc gccgcgtctg agccttgctg tttataacaa gtgccaacgg
    20341 cggcgcgttc atcgttattc attaacggca ggttgatagc ggcgggcatt gcgccttgct
    20401 caaactcgat aggggcgcga acatcaataa tgggcgtatc agcaatcagc agggcacgat
    20461 agtcctgttc cgtgtgtctc tcttgcatag ttaaaagtga acctcaaatc agcttgcgcg
    20521 ctattttacg cgccaacgcg caaggaaact tgatttttaa ctgcgtgggt tgccgaaaat
    20581 ttctaaaaat ccgctgattt ccggcctgcg ctgggtaaac aaggtcacaa tatcttctac
    20641 cgctttgccg ctgccaaatt tgcgccatgc cagactcaat ggcgaaggag ggcgcatcgt
    20701 tgggattacc cggctgacca gttgttgatt atcgatcatt gactggcaaa gtgattttgg
    20761 caaaaaacca atgccaacac ccgccagatg ggcggcgatt ttcgtttcca tatcgggaac
    20821 aataatctct ttttgccccg gcaatcgcca ggcgacgcgt ttggttaagg ttcgggcgct
    20881 gtcttcaata ttgaccgccg gaaagcgccg caactgcgct tctgttagcg gctcttcaac
    20941 gttcgccagc ggatgatccg ccgccatgac aaagcgccat tgcaccgatc ctaagggatc
    21001 aagactaaag gtatttgcca gcgcctcagt tcccgtgacc ccgatagcca gcgaaaaacc
    21061 ttcgtacaat agcgagtccc agacgcccat atagatttgt cgggagatgt gaaactgggt
    21121 aaaggggtaa cgctcattca gccacgccag caactgggcg acggcctggg ggttatagag
    21181 caggttgttg atgacaatat tcacctagcg ttccacgcca tcattcacct gttgcagctc
    21241 gctgggcata ctttccagcc agctcagcca gtctctggcc tgggaaagta gatgctcgcc
    21301 agccgctgtc aacgtcacgc tgcgagtcgt gcggaaaaac agcgccactc cggtattctc
    21361 ttccagaagt ttaatgcgat aactgatcgt cgccgtggtt ttacataatc gttctgccgc
    21421 ttttgaaaaa cttcctgttt cagcaaccgc aatgaaagtc cgcaaggttt ctggatcgaa
    21481 catcttcagg tatccccttt taaatccgca agttgcgtga ttttcttatc ctctgattta
    21541 tcagtatttt tacatgataa ccctgttcaa tttgtggact aaatctagtt ttggaaaaat
    21601 attccaactt ttgtattgat gttgttctct taaggtttta gat
//
LOCUS       DAESHI010000070        21005 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.070, whole genome shotgun sequence.
ACCESSION   DAESHI010000070 DAESHI010000000
VERSION     DAESHI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.070"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..170)
                     /locus_tag="KW011_002681"
     CDS             complement(<1..170)
                     /locus_tag="KW011_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7660981.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYG"
     gene            342..1562
                     /gene="coaBC"
                     /locus_tag="KW011_002682"
     CDS             342..1562
                     /gene="coaBC"
                     /locus_tag="KW011_002682"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBH7660982.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1540..1998
                     /gene="dut"
                     /locus_tag="KW011_002683"
     CDS             1540..1998
                     /gene="dut"
                     /locus_tag="KW011_002683"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBH7660983.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2105..2701
                     /gene="slmA"
                     /locus_tag="KW011_002684"
     CDS             2105..2701
                     /gene="slmA"
                     /locus_tag="KW011_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HBH7660984.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="KW011_002685"
     CDS             complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="KW011_002685"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBH7660985.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3445..4161)
                     /gene="rph"
                     /locus_tag="KW011_002686"
     CDS             complement(3445..4161)
                     /gene="rph"
                     /locus_tag="KW011_002686"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBH7660986.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4288..5151
                     /locus_tag="KW011_002687"
     CDS             4288..5151
                     /locus_tag="KW011_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBH7660987.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5373..6197
                     /gene="dinD"
                     /locus_tag="KW011_002688"
     CDS             5373..6197
                     /gene="dinD"
                     /locus_tag="KW011_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HBH7660988.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6489..7106
                     /locus_tag="KW011_002689"
     CDS             6489..7106
                     /locus_tag="KW011_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HBH7660989.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="KW011_002690"
     CDS             complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="KW011_002690"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HBH7660990.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            9043..9666
                     /gene="gmk"
                     /locus_tag="KW011_002691"
     CDS             9043..9666
                     /gene="gmk"
                     /locus_tag="KW011_002691"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBH7660991.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9721..9996
                     /gene="rpoZ"
                     /locus_tag="KW011_002692"
     CDS             9721..9996
                     /gene="rpoZ"
                     /locus_tag="KW011_002692"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBH7660992.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            10015..12123
                     /gene="spoT"
                     /locus_tag="KW011_002693"
     CDS             10015..12123
                     /gene="spoT"
                     /locus_tag="KW011_002693"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HBH7660993.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            12130..12819
                     /gene="trmH"
                     /locus_tag="KW011_002694"
     CDS             12130..12819
                     /gene="trmH"
                     /locus_tag="KW011_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HBH7660994.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12825..14906
                     /gene="recG"
                     /locus_tag="KW011_002695"
     CDS             12825..14906
                     /gene="recG"
                     /locus_tag="KW011_002695"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBH7660995.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="KW011_002696"
     CDS             complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="KW011_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HBH7660996.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            16560..17951
                     /gene="xanP"
                     /locus_tag="KW011_002697"
     CDS             16560..17951
                     /gene="xanP"
                     /locus_tag="KW011_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HBH7660997.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            18072..19781
                     /locus_tag="KW011_002698"
     CDS             18072..19781
                     /locus_tag="KW011_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBH7660998.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKHQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(19833..>21005)
                     /gene="yicI"
                     /locus_tag="KW011_002699"
     CDS             complement(19833..>21005)
                     /gene="yicI"
                     /locus_tag="KW011_002699"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HBH7660999.1"
                     /translation="GLAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQ
                     WFDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCY
                     ANYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGS
                     KSYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPA
                     CDYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLS
                     LPVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKA
                     TRTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT         5083 a         5720 c         5385 g         4817 t
ORIGIN      
        1 ccataaagag agccgaaatt ctccagcatc tcttttgcca gggttaatac atctttaccg
       61 cgcgttccgg tacgcagaaa tagcgccagc agctcgacat ccgttaaggc gctaatacca
      121 aacttcagca ttttttcgcg cggcatcaac agctgtgcat tgtttttcac cttcacctcc
      181 tttgtggtgc tcgcatcctg tcatagccgt ttcgttaaat cgacggccag ttttcaatcc
      241 tggaaagcgc ctcgcaaagt gaatctcagg tgaaggcacg acaacaaaag tattgtgata
      301 aaatcgccaa cttctggtgt caccctacag gaaaaatcat catgagcctg gccggtaaaa
      361 aaatcgttct cggcgttagc ggcggtattg ctgcctataa aacccctgaa ctggtgcgtc
      421 gtttgcgcga tcgcggggcc gacgtccgcg tagccatgac cgaagcggca aaagccttta
      481 tcaccccact tagcttgcag gcggtttctg gttatcccgt ttccgacagt ctgctggacc
      541 cggcagccga agccgctatg ggccatattg agctgggtaa atgggctgat ttagtgattc
      601 tcgcccctgc cacggcagat ttgattgccc gtgttgctgc cggaatggcg aatgacctgg
      661 tatcgacgat ttgtctggct acacctgcgc ctgtagccgt gctccccgcc atgaaccagc
      721 agatgtaccg tgccgctgcc acgcagcata atttagaggt gcttgcttcc cgtggtttgc
      781 tcatctgggg gccagacagt ggcagtcagg cttgtggtga tatcggtcct gggcgaatgc
      841 tcgatccgtt aaccattgtg gatatggcgg tagcgcattt ttcgcccgtc aacgacctga
      901 aacatctgaa cattatgatt accgccggcc cgacgcgtga accgctcgat ccggtgcgtt
      961 atatctctaa tcacagctcc ggcaagatgg gttttgctat cgccgccgcc gctgcccgtc
     1021 gtggcgcgaa cgtcacgctg gtatcaggcc cggtttcgct accgacgcca ccgtttgtta
     1081 aacgtgttga tgtgatgacc gcgctggaaa tggaagtcgc cgtgaatgct tctgtacagc
     1141 agcaaaatat ttttatcggc tgcgccgccg tggcggatta tcgcgcagct accgtggccc
     1201 cagagaaaat caaaaagcag gccacgcagg gtgatgaatt aacaataaaa atggttaaaa
     1261 accccgatat cgtcgcaggc gttgccgcac taaaagacca tcgaccctac gtcgttggat
     1321 ttgccgccga aacaaataat gtggaagaat acgcccggca aaaacgtatc cgtaaaaacc
     1381 ttgatctgat ctgcgcgaac gatgtttccc agccaactca aggatttaac agcgacaaca
     1441 acgcattaca ccttttctgg caggacggag ataaagtctt accgcttgag cgcaaagagc
     1501 tccttggcca attattactc gacgagatcg tgacccgtta tgatgaaaaa aatcgacgtt
     1561 aagattctgg acccgcgcgt tgggaaggaa tttccgctcc cgacttatgc cacctctggc
     1621 tctgccggac ttgacctgcg tgcctgtctc gacgacgccg tagaactggc tccgggtgac
     1681 actacgctgg ttccgaccgg gctggcgatt catattgccg atccttcact ggcggcaatg
     1741 atgctgccgc gctccggatt gggacataag cacggtatcg tgcttggtaa cctggtagga
     1801 ttgatcgatt ctgactatca gggccagttg atgatttccg tgtggaaccg tggtcaggac
     1861 agcttcacca ttcaacctgg cgaacgcatc gcccagatga tttttgttcc ggtagtacag
     1921 gctgaattta atctggtgga agatttcgac gccaccgacc gcggtgaagg cggctttggt
     1981 cactctggtc gtcagtaaca catacgcatc cgaataacgt cataacatag ccgcaaacat
     2041 ttcgtttgcg gtcatagcgt gggtgccgcc tggcaagtgc ttattttcag gggtattttg
     2101 taacatggca gaaaaacaaa ctgcgaaaag gaaccgtcgc gaggaaatac ttcagtctct
     2161 ggcgctgatg ctggaatcca gcgatggaag ccaacgtatc acgacggcaa aactggccgc
     2221 ctctgtcggc gtttccgaag cggcactgta tcgccacttc cccagtaaga cccgcatgtt
     2281 cgatagtctg attgagttta tcgaagatag cctgattact cgcatcaacc tgattctgaa
     2341 agatgagaaa gacaccacag cgcgcctgcg tctgattgtg ttgctgcttc tcggttttgg
     2401 tgagcgtaat cctggcctga cccgcatcct cactggtcat gcgctaatgt ttgaacagga
     2461 tcgcctgcaa gggcgcatca accagctgtt cgagcgtatt gaagcgcagc tgcgccaggt
     2521 attgcgtgaa aagagaatgc gtgagggtga aggttacacc accgatgaaa ccctgctggc
     2581 aagccagatc ctggccttct gtgaaggtat gctgtcacgt tttgtccgca gcgaatttaa
     2641 ataccgcccg acggatgatt ttgacgcccg ctggccgcta attgccgcgc agttgcagta
     2701 atatgacgcc ggatgacttt tcatccggcg agtttcttta aacgccaaac tcttcgcgat
     2761 aggccttaac cgccgccaga tgttccgcca tttccggctt ctcttccagg taagcaatca
     2821 ggtctttcag ggtgatgata gagatcactt tgcagttgta atcacgctca acttcctgaa
     2881 tcgccgaaat ctcgctgcgc ccccgttcct gacgatcgag cgaaatcaac acgccagcaa
     2941 gcgtcgcgcc attggcctga ataatttcca tcgactcacg aatcgccgtt ccggcggtga
     3001 tcacatcatc taccagcatt acgcgtcctt gtaacgcgct accaaccaga ttgccgcctt
     3061 caccgtggtt ttttgcctct ttgcggttaa agcagtacgg caggtccagg tcatgatgct
     3121 ccgccagcgc cacggcggtt gtggtggcaa tcgggatccc tttgtaagca gggccaaaca
     3181 gcagatcgaa ctcaatgccg gaatccacca atgcttcagc gtaaaaacgg cctaacagtg
     3241 ccaggtcgcg cccggtatta aacagcccgg cgttgaagaa ataggggctt ttacgcccgg
     3301 atttcagcgt aaactcgcca aactttaaca cctgcttgct aagcgcaaat tcaataaact
     3361 ggcgctgata tggtttcatg ccttcgctcc tcatcttact tttctacaga caaaaaaaag
     3421 gcgactcatc agtcgcctta aaaatcagtt tgccagcgcc gccttctgcg tcgctacaat
     3481 ggattcgatt ccccctcggg ccagagccaa caaggtgagt aattcttcgt gggtgaacgg
     3541 ttcgccttct gccgtcccct gcacttcaat gatgcgcccg tcttcggtca tcactacgtt
     3601 catgtcggtc tctgcggcag agtcttcaac gtattccaga tcgcaaaccg cttcgccgtt
     3661 cacaatcccg acagaaaccg cggctaccat ccctttcatc ggattggttt tcagcttacc
     3721 gttttccacc agcttctgta gcgcatctgc cagcgccacg caggcacccg taatcgaagc
     3781 ggtacgagtg ccaccatcag cctgaagcac gtcgcagtcc agcgtaatgg tgaactcacc
     3841 cagcgctttc aaatctactg ccgcgcgaag cgcacgggcg atcagacgct ggatttccat
     3901 tgtgcgtcca ccctgcttac ctttcgccgc ttcacgagcg ttacgggtgt gggtagaacg
     3961 cggcagcata ccgtactctg cggtgatcca gccctggccc tgacctttca ggaagcgcgg
     4021 cacgccttct tcaatagagg cggtacacaa cactttggta tcgccaaatt cgaccagcac
     4081 cgagccttct gcatgttttg tatagttacg agtcagggta acgggacgca cctgattatt
     4141 gctacggcct gctggacgca tattgaaatc tccggcttga aacaaatgtg gctgcgcatt
     4201 atacggactt ccggaggtta ttcctatcct gacaaggcat cgatggctat aatccttcca
     4261 cctctccttt tataaacagg aacgtctatg atccgcagta tgaccgccta cgcccggcgt
     4321 gaaatcaagg gtgaatgggg gagcgcaacc tgggaaatgc gctcggtaaa ccagcgttat
     4381 ctggaaactt actttcgtct gccggagcag ttccgtagcc ttgaacctgt cgttcgcgag
     4441 cgtattcgtt ctcgcctgac gcgcggtaaa gtggaatgta ccctgcgcta tgagccagat
     4501 gttagcgcgc aaggtgagct gatcctcaac gaaaaactgg ctaaacagct ggtaactgcc
     4561 gcgaactggg taaaaatgca gagtgacgaa ggggaaatca acccggttga tattctgcgc
     4621 tggccgggcg taatggcagc ccaggagcag gatctcgacg cgattgccgc tgaaattctc
     4681 gcggcgttgg atggtaccct ggacgacttt atcgtcgcac gtgaaaccga aggtcaggcg
     4741 ctgaaagcgt tgatcgagca gcgtctggaa ggcgtcaccg ccgaagtggt caaagtccgc
     4801 gcccatatgc cggaaatcct gcaatggcag cgtgagcgtc tggtcgcgaa gctggaagat
     4861 gctcaagtgc aactggaaaa caaccgcctg gagcaggagt tggttctgct ggcacaacga
     4921 attgatgttg ccgaagaact ggaccgcctg gaagcgcatg tcaaagagac ctacaacatt
     4981 ctgaagaaaa aagaagccgt tggtcgtcgt ctggacttta tgatgcaaga gttcaaccgc
     5041 gagtcgaaca ctcttgcgtc gaaatctatc aatgccgaag tgacgaattc tgccatcgag
     5101 ctgaaagtgt tgattgagca gatgcgcgag cagattcaga atattgagta actcgtaaca
     5161 ctaattctta cctttcagcc ttcacaaatg ccgcccactc aaacagagcg gcatttttct
     5221 tccccgcaac attcaattct gttttgcgta cctgctccag attttgcgat gtttttttgc
     5281 gcagcgcagt gagaacgtga gatactcaca actgtatata aatacagtta tagacttact
     5341 ttctttgcaa tcgatatcac atggagtggg caatgaacga acatcatcaa ccttttgaag
     5401 aaatacggca ttatggcact gaagggcaag aattttggtc agcgagagaa ttggcaccgt
     5461 tactggatta tcgggactgg cgcaattttc aaaaggtact ggcccgcgcg actcaagcgt
     5521 gcgaagccag caatcaagct gcatctgacc atttcgttga aaccaccaaa atggttgtat
     5581 taggttcagg ggctcaacga gaacttgaag atgttcatct ctctcgctat gcctgttacc
     5641 tggtagtaca aaacggcgac cctgcgaaac cggttattgc ggcagggcaa acttattttg
     5701 ctatccagac ccgacggcag gagctggctg acgatgaagc tttcaggcaa cttcgtgaag
     5761 acgaaaagcg tctttttctg agaaatgagt tgaaagaaca taacaaacaa ttagttgagg
     5821 cagcgcagca agctggggta gcgacagcta tcgatttcgc catatttcag aatcatggtt
     5881 accgggggct gtatggtgga ttagatcaga aagccatcca tcagcggaag gggctgaaaa
     5941 agagtcagaa gatcctggat catatgggtt caacagaact ggcggctaat ctctttcgag
     6001 ctacccaaac agaagaaaaa ctcaagcggg atggcgtaaa ttcaaaacag caagctaata
     6061 ccacacactt tgacgtgggt agcaaggtga ggcaaaccat tcaggaactt ggcggaacca
     6121 tgcctgagga gttgccgacc ccgcaggtca gcatcaatca gttggaaaac agtgtaaaaa
     6181 ttacagagaa gaaatagcag aaactaaaaa ctctttttct taattgagat acccgatgcg
     6241 taagccaaag tccaggtgca gtgaacacaa tggctaaaca ttgcaccttt cttttccacc
     6301 cctcaatttt aacctatttt ttcttatgca ttttctcaga caagaagtca gaagaatgca
     6361 tctacactac agaaaatagc gatttcacat aactacaagt tatcaatttc ctctcacctt
     6421 aaaaaatctc aatcgtgaca atacgcacaa atcgctaccc tgccagacag atttttaggg
     6481 agagaaccat gctgttacac attttgtatt tggttggcat tactgccgaa gccatgacgg
     6541 gggcgctggc ggccggacga cggcgcatgg atacatttgg cgtaattatt attgcgaccg
     6601 ccaccgcaat tggcggaggg tcagtgcgcg atattctgct gggccactat ccgctcggct
     6661 gggtcaaaca cccggaatat gtgattatcg tcgctaccgc cgcagtactc accactatcg
     6721 ttgctcctgt aatgccttac ctgcgcaaag tgtttctggt gctcgatgcg ctcggactgg
     6781 tggtcttttc tatcatcggc gcacaggttg cactggatat ggggcacggc ccaattattg
     6841 ccgttgtcgc ggcggtaacc accggcgtgt ttggcggcgt tttgcgcgat atgttctgta
     6901 aacgcatccc actggtattc cagaaagagt tgtatgccgg tgtctcattt gcctccgccg
     6961 tgctgtacat cgcgctgcaa cactatgttt ctaaccatga tgtggtgatt atctccaccc
     7021 tggtattcgg cttttttgcc cgcttactgg cgctacgtct taagctggga ttaccggtct
     7081 tttactacag ccacgaaggt cactaaggtt caaaacctgt gatctgctgg gcagccagcc
     7141 aactgcccag cttcttgatt tgcgcatttt ccttccattc aataacctgt ctggcgcgcc
     7201 cagatccagt ccccggcagc cgctgccaga actgctccgt gctaaataaa agttgcgacc
     7261 aggaccgttc atcactggca ttaagcgccg cccgggttaa cggtattccc attgccatca
     7321 cccagcgagt aaaaggctgc ttacgagcca gattaaactg atgccataac tgcgcacttt
     7381 tacttttcgc gatccccggc gtgttctgta attgctctgg cgttaataaa agccaggaaa
     7441 agatatgctc aaagcgatga gtctgatgca gcgcgcgcca gccggcctca ccaatgccat
     7501 ccagcccaag aacctgtttt gaccccagcc agactaagcg tgaaatgaac tgttcctgac
     7561 aaacatcaga agcaaagtag caggtcaacg agttaaagcg gttttctggt ggtgtcggtt
     7621 ttgtacgttc tgcaccgcgc cagaccacat catcaatgcg aggaataccc tgaccggcaa
     7681 ggctgacgag gatctgatca ccaggcgcaa tatcccactc ctgccagcgc ctgacggaac
     7741 caatattcac ccgctggact tttttatcat ccagcatgac aggtgcgagt gacgcaacca
     7801 ccgatatttt accgctctta cccaccgcaa actgaatcgc cttcacttcg gcaacctgag
     7861 ctacaggttg atatttccag gccaccaacc attctgcctg gcctggtagc caatggcggg
     7921 attcgggttc cttagccgct cgcacaacca cgccatcggt gacgaagggt aatttcgctt
     7981 tccaccactc attgcgtacg cgcgcaactt catcagcatt tttaaccgca cgggtatacc
     8041 tctgcgttag agtaaaacct gcggtagcca gctcctttaa acgatcggtc attaactgcg
     8101 gtccatccgg ccatgcccag acaaaaacag ccagtgagtt tagcgtgtcg ctattcccct
     8161 ggcgcatcat caggccagca acttttgcgc gggcatttat tccccccatt tgttgttgga
     8221 tatgcccctc gcgctggaga aatatttccc cctgaagcgt actgttggct aaaggcccgc
     8281 taacggtttg cggcacagcg gaaattaagc taactttctg cgtccagtcc tcgcctttca
     8341 ggccattacc gcgactgatt gctttgttca gtttcccgtc ccgataaacc agggttaccg
     8401 caacgccatc aacttttggc tgcacccaaa gatcgctacg ttctcgcatc cacagactta
     8461 atgcgttttt atccaccatt ttgcgtacac ccgtatgggc aaccggatgc ataaccgcac
     8521 catttaaagg cggcatcatc acgtcgcggg gctcgctccc aaagcagcgt tgccactgcg
     8581 taagacgggc gcttaactga tcgtaaacac cgtcttccac ctcacttttc ccttccttcc
     8641 agtagtcatc gtcccactgt tttatttgct gttgcaggcg ggaaatttct tcctgtgctc
     8701 tggctggcga ccaggccgga cagaccgccc acacagatga ttgccagcac aagatactta
     8761 ttaatatcgc catccatact ttcatcatca cctccgctgt agatagtcag gcagatatac
     8821 aacgtgatga aagcaaagcc gagtggcaaa aacggagtct gcgaggacgc ttcctgaaaa
     8881 tcgtctttat tgcaatgaat gacaggcaaa tgcggaagca gctacgcaaa acgcaacaac
     8941 tttgcgcaaa aagtgtgagc aagggctacg tcacatggcc gcgccgtgta taataagctc
     9001 gtatgtaggc tttatttcgc taatcacata cgaaagatac tcatggctca aggcacgctt
     9061 tatattgttt ctgcccccag tggtgcgggt aaatccagcc tgattcaggc tttattaaaa
     9121 acccaaccgt tgtatgacac ccaggtttct gtttcacaca ccacacgcca accgcgtcct
     9181 ggtgaagtcc acggtgaaca ttatttcttt gttaatcatg atgaatttaa agaaatgatt
     9241 agcagagatg cgttcctcga acacgcagaa gtttttggta attactatgg cacttcgcgt
     9301 gaggccattg agcaagtact ggcgaccggt gtcgatgttt ttctcgatat cgactggcag
     9361 ggcgcgcagc aaattcgcca gaagatgccg cacgcgcgga gtatctttat tttaccgccg
     9421 tccaaaattg aactggaccg ccgtctacgc ggtcgcggtc aggacagcga agaggtcatt
     9481 gcaaagcgta tggcgcaagc tgttgcagaa atgagccatt acgccgaata tgattatctg
     9541 attgtgaatg atgacttcga taccgcgttg accgatttga agaccattat tcgcgccgaa
     9601 cgtctgcgca tgagccgcca aaagcagcgt catgacgctt taatcagcaa attgttggca
     9661 gactgaacct gatttcagta tcatgcccag tcatttcttc acctgtggag ctttttaagt
     9721 atggcacgcg taactgttca ggacgctgta gagaaaattg gtaaccgttt tgacctggta
     9781 ctggtcgccg cgcgtcgcgc tcgtcagatg caggtaggcg gaaaggatcc gctggtaccg
     9841 gaagaaaacg ataaaaccac tgtaatcgcg ctgcgcgaaa tcgaagaagg tctgatcaac
     9901 aaccagatcc tcgacgttcg cgaacgccag gaacagcaag agcaggaagc cgctgaatta
     9961 caagccgtta ccgctattgc tgaaggtcgt cgttaatcac aaagcgggtc gcccttgtat
    10021 ctgtttgaaa gcctgaatca actgattcaa acctacctgc cggaagacca aatcaagcgt
    10081 ctgcggcagg cgtatctcgt tgcacgtgat gctcacgagg ggcaaacacg ttcaagcggt
    10141 gaaccctaca tcacgcaccc ggtagcggtt gcctgcattc tggccgagat gaaactcgac
    10201 tatgaaacgc tgatggcggc gctgctgcat gacgtgattg aagatacacc cgccacctac
    10261 caggatatgg aacagctttt tggtaaaagc gtcgccgagc tggtagaggg ggtatcgaaa
    10321 cttgataaac tcaagttccg cgataagaaa gaggcgcagg ccgaaaactt tcgcaagatg
    10381 attatggcga tggtgcagga tatccgcgtc atcctcatca aacttgccga ccgtacccac
    10441 aacatgcgca cgctgggctc acttcgcccg gacaaacgtc gccgcatcgc ccgtgaaact
    10501 ctcgaaattt acagcccgct ggcgcaccgt ttaggtatcc accacattaa aaccgaactc
    10561 gaagagctgg gttttgaggc gctgtatccc aaccgttatc gcgtaatcaa agaagtggtg
    10621 aaagccgcgc gcggcaaccg taaagagatg atccagaaga ttctttctga aatcgaaggg
    10681 cgtttgcagg aagcgggaat accgtgccgc gtcagtggtc gcgaaaagca tctttattcg
    10741 atttactgca aaatggtgct caaagagcag cgttttcact caatcatgga catctacgct
    10801 ttccgcgtga tcgtcaatga ttccgacacc tgttatcgcg tgctcgggca aatgcatagc
    10861 ctgtacaagc cgcgtccggg tcgcgtgaaa gactacatcg ccattccaaa agcgaacggc
    10921 tatcagtctt tgcacacctc gatgatcggc ccgcacggtg tgccggttga ggtccagatc
    10981 cgtactgaag atatggatca gatggcggag atgggtgttg ccgcgcactg ggcttataaa
    11041 gagcacggcg aaaccagcac caccgcgcaa atccgcgccc agcgctggat gcaaagcctg
    11101 ctggagctgc aacagagcgc cggtagttcg tttgaattta tcgagagcgt taaatccgat
    11161 ctcttcccgg atgagattta cgttttcaca ccggaagggc gaattgtcga gctgcctgcc
    11221 ggtgcaacgc ccgtcgactt cgcttatgca gtgcataccg atatcggtca tgcctgcgtg
    11281 ggcgcacgcg ttgaccgcca gccttacccg ctgtcgcagc cgcttaccag cggtcaaacc
    11341 gttgaaatca ttaccgcacc gggcgctcgc ccgaatgccg cgtggctgaa ctttgtcgtt
    11401 agctcaaaag cgcgcgccaa aattcgtcag ttgctgaaaa acctcaagcg tgatgattct
    11461 gtaagcctgg gccgtcgtct gctcaaccat gctttgggtg gtagccgtaa gctgaatgaa
    11521 atcccgcagg aaaatattca gcgcgagctg gatcgcatga agctggcaac gcttgacgat
    11581 ctgctggcag aaatcggact tggtaacgca atgagcgtgg tggtcgcgaa aaatctgcaa
    11641 catggggacg cctccattcc accggcaacc caaagccacg gacatctgcc cattaaaggt
    11701 gccgatggcg tgctgatcac ctttgcgaaa tgctgccgcc ctattcctgg cgacccgatt
    11761 atcgcccacg tcagccccgg taaaggtctg gtgatccacc atgaatcctg ccgtaatatc
    11821 cgtggctacc agaaagagcc agagaagttt atggctgtgg aatgggataa agagacggcg
    11881 caggagttca tcaccgaaat caaggtggag atgttcaatc atcagggcgc gctggcaaac
    11941 ctgacggcgg caattaacac cacgacttcg aatattcaaa gtttgaatac ggaagagaaa
    12001 gatggtcgcg tctacagcgc ctttattcgt ctgaccgccc gtgaccgtgt gcatctggcg
    12061 aatatcatgc gcaaaatccg cgtgatgcca gacgtgatta aagtcacccg aaaccgaaat
    12121 taatgtttta tgaacccaac acgttatgca cgcatctgcg aaatgctcgc caggcggcag
    12181 cctgatctga ccgtctgcat ggagcaggtc cacaaacctc ataacgtttc tgcgattatt
    12241 cgtaccgcag atgccgttgg cgtacatgaa gttcacgccg tctggcctgg tagccgcatg
    12301 cgcaccatgg cttcggcagc ggcgggtagt aacagctggg tacaggtgaa aacacaccgc
    12361 accattggcg atgccgtcgc tcatctgaaa ggccagggca tgcagattct ggcaacccat
    12421 ctttctgata acgctgtcga tttccgcgaa attgattaca ctcgcccgac ctgcattttg
    12481 atgggacagg agaaaacggg catcacgcag gaagcattgg ccctggcgga tcaggacatc
    12541 atcattccga tgatcggcat ggtgcagtcg ctgaatgttt ccgttgcctc agccctcatt
    12601 ctttacgaag cccagcgtca gcggcaaaat gcaggcatgt acctgcgtga aaacagcatg
    12661 ttgccggaag cagagcaaca acgcctgttg tttgaaggcg gctatccggt gctggcgaaa
    12721 gtcgcaaaac gcaaaggcct gccttatccc cacgtcaatc agcaaggcga gatcgaagct
    12781 gatgccgact ggtgggctac tatgcaggct gcagggtaag tgccatgaaa ggtcgcctgt
    12841 tagatgctgt cccactcagt tccctaacgg gcgttggcgc agcacttagt aacaaactgg
    12901 cgaaaatcaa cctgcatacc gtgcaggatc tactcttaca ccttcccctg cgctacgaag
    12961 atcgcaccca tctctacccc atcggagaac tactgccggg cgtttatgcc acggtggaag
    13021 gcgaagtgct gaactgcaat atctccttcg gcggtcggcg gatgatgacc tgccagatca
    13081 gcgacggttc cggcatcctc accatgcgct ttttcaattt cagcgcggca atgaaaaata
    13141 gcctggcgac gggccgccgt gtactggctt atggcgaagc aaagcgcggt aaatatggtg
    13201 cggagatgat ccacccggaa taccgcgtgc agggcgatct cagcacgcca gaattacagg
    13261 aaacgctcac gccggtttat ccaacaacgg aaggcgtaaa gcaggccacg ctgcgtaaat
    13321 taaccgacca ggcgctggat ctgctcgaca cctgcgccat tgaagaactc ctgccgccgg
    13381 aactgtcaca aggaatgatg acgctaccgg aagcgttgcg cactttgcac cgcccgccac
    13441 cgacgctaca gcttagcgat ctggaaaccg ggcagcatcc ggcgcaacgt cgtctgattc
    13501 tggaagaact gctggcgcac aacctcagca tgttagcctt acgtgccgga gcacagcgtt
    13561 ttcatgccca gccgctgagc gccaatgatg cgctgaaaaa taaactcctc gcagccttac
    13621 cgttcaagcc aacgggcgca caggcacgcg tagtggcgga gatcgagcgc gatatggcgc
    13681 tggatgtgcc gatgatgcgc ctggtacagg gcgatgtagg ttccggtaaa acgctggtcg
    13741 ccgccctcgc cgcgctgcgt gcgattgccc acggcaaaca ggtggcattg atggcaccaa
    13801 ccgaattact cgccgaacag cacgccaata acttccgcaa ctggtttgaa ccgctcggta
    13861 tcgaagtggg ctggctcgcc ggtaagcaga aaggcaaagc gcgactgtct cagcaggaag
    13921 ccatcgccag cggtcaggtg cagatgattg ttggtacgca cgccatcttc caggaacagg
    13981 tgcagtttaa cggtctggcg ctggtgatta tcgacgaaca acatcgcttt ggcgtgcatc
    14041 agcgtctggc gttatgggag aaaggccagc agcaaggctt ccatccgcat cagttgatca
    14101 tgaccgccac gccgatcccc cgcacgctgg caatgacggc gtatgccgat ctcgatacct
    14161 cggtgataga tgaactgccg ccaggccgca cgccagtgac tacggtcgct attcctgata
    14221 ctcgccgtac cgacatcatt gaccgcgtgc gccacgcctg catgactgaa ggtcgtcagg
    14281 cgtactgggt ttgtacgttg attgaagagt cagagttact ggaagcgcag gcggcggaag
    14341 ctacctggga agagttgaaa ctggcgctac cagaattgaa tgttggcctg gtacacgggc
    14401 ggatgaaacc agccgagaaa caggcggtga tggcgtcgtt taaacaaggt gagctacatc
    14461 tgctggttgc cactaccgtt attgaagtcg gtgttgatgt gcctaacgcc agcctgatga
    14521 ttatcgaaaa ccctgagcgt ctgggtctgg cgcagttaca ccagctgcgc gggcgcgtag
    14581 gtcgtggcgc ggtggcttct cactgcgtgc tactctacaa aacgccgctt tctaaaacgg
    14641 cgcaaattcg cctgcaagtg ctgcgcgaca gtaacgacgg ttttgtgatt gcgcaaaaag
    14701 atctggagat tcgcggccct ggcgaattgt taggcacgcg tcagacgggt aatgctgaat
    14761 ttaaagtggc ggatttactg cgcgatcagg cgatgatccc ggaagttcag cgcctggcac
    14821 gccatattca cgaacgttac ccacaacagg caaaagccct gatagaacgc tggatgccgg
    14881 agacggaacg ttactcgaat gcgtaaaagg cggcagtgag aagaccgcca tttcaggtta
    14941 ccctaccttc ctgccggatg cgattcatca ccctacaaat tcaataaatt atgaatcaat
    15001 acgcaggctt gataagcgaa gcgtatcagg catttgtctt tgttgcggcg gcacttctac
    15061 cgccgcttca tcggttaacc ggcaaaaatc ggcaacatca aatacaactt aattaccagc
    15121 gcattgacga tatcgataaa gaacgcaccg accatcggca ccaccaaaaa cgccatgtgc
    15181 gacgggccaa agcgttcagt aatcgcctgc atgttggcga ttgccgttgg cgttgcaccg
    15241 aggccaaaac cacagtgacc tgcagccagc actgccgcat cgtagttttt gcccatcatg
    15301 cgccaggtaa cgaagatggc atacaacgcc atgaagatgg tctgtaccac cagaatcgcc
    15361 agcatcggca gcgccagcga agccagctcc cacagtttca gccccatcaa cgccatcgcc
    15421 aggaacaacg acaagcttac gttacccagc acggataccg cacgctcaaa gacgcggtaa
    15481 aagcccatta ttgacagacc gttgctcaga atcacgccaa caaacagtac acagacgaag
    15541 gtcggcagtt caaaagcagt gccagccaaa agttgcgcaa caattttccc caccgtcagg
    15601 cagatagcaa tcagcgcgat agtttcaatc agcaccaacg aggtgatcat gcgtcccaca
    15661 tccggctttt caaacgccgt cgggacttcc tggtcatccg gaataccgtt cggcgtggtg
    15721 gagtgtttca ccagatagcg cgccaccgga ccgccaatca agccgcccag caccagaccg
    15781 aacgttgcac aggccatcgc cacttccgtc gcattggtga agccataacg ttcaatgaac
    15841 aatttactcc acgcagcgcc cgtaccgtga ccgccggaaa gcgtaataga accggccaac
    15901 agccccatca gcggatcaag ccctaacagg ctagccatac caatgccaat ggcattttgc
    15961 atcaccaaca gaccaacaac cacaatcaag aagatgccaa ccacacgccc accggcacgc
    16021 aaactggcaa tgttggcgtt caggccaatg gtggcgaaga aagccagcat taacggatcg
    16081 cgcagggaca tatcaaagtt gacttcccag cccatgcttt ttttcagtac tagtagcgcc
    16141 agcgccacca acaaaccacc cgcaacaggt tccggtatgg tgtatttctt caaaaaggag
    16201 acggaatgga ccaacttacg cccgagcagc aacgtcagcg ttgcggcaac aagcgttgct
    16261 aaagtatcga gatgaaacat agttactcct ttgtatccgc atcactcttt catactcgtc
    16321 atacttcatc gcgcagtggc aacttcatga actatgacgg gtatcgagtt atgctttcct
    16381 gggtcaaatt ctcagctgtt aatgaacaaa tggcatggat tttaagcaga aaaataccaa
    16441 aagttatata aaaaagtgca tttatctcat ttaaatattt agatgaggat gtggcaaacg
    16501 tttgcttttc tctttcggtc agctaaaatg cccgctttgc taccacggga ttgttttcga
    16561 tgtctgtttc caccctcgag tcagaaaatg cgcaaccggt tgcgcagact caaaacagcg
    16621 aactgattta ccgtcttgaa gatcgtccgc cgcttcctca aaccctgttt gccgcctgtc
    16681 agcatctgct ggcgatgttc gttgcggtga tcacgccagc gctattaatc tgccaggcgc
    16741 tgggtttacc ggcacaagac acgcaacaca ttattagtat gtcgctgttt gcctccggtg
    16801 tggcatcgat tattcaaatt aaggcctggg gtccggttgg ctccgggctg ttgtctattc
    16861 agggcaccag cttcaacttt gttgccccgc tgattatggg cggtaccgcg ctgaaaaccg
    16921 gtggtgctga tgttcctacc atgatggcgg ctttgttcgg cacgttgatg ctggcaagtt
    16981 gcaccgagat ggtgatctcc cgcgttctgc atctggcgcg ccgcattatt acgccgctgg
    17041 tttctggcgt tgtggtgatg attatcggcc tgtcgctaat tcaggtgggg ctgacctcca
    17101 ttggcggcgg ttacgcagcc atgagcgata acaccttcgg cgcaccgaaa aatctgctgc
    17161 tggcaggcgt ggttttagcc ttaattatcc tgcttaaccg tcaacgtaac ccttacttac
    17221 gcgtggcctc actggtaatt gcgatggcgg ccggatatgc gctggcgtgg tttatgggca
    17281 tgttgccaga aagcaacgaa ccgatgacgc aagaactgat tatggtgcca acgccgctct
    17341 attacggtct tggcattgaa tggagtctgc tgctgccgct gatgctggtc tttatgatca
    17401 cttcgctgga aaccattggc gatatcacgg cgacctctga cgtttccgaa cagccagtgt
    17461 ccggtccgct gtacatgaaa cgcctgaaag gcggcgtgct ggcaaacggc ctgaactcgt
    17521 ttgtttcggc ggtgtttaac accttcccga actcctgctt cgggcaaaac aacggagtga
    17581 tccagttgac cggtgttgcc agccgctatg tcggttttgt cgtcgcgctg atgttgatcg
    17641 tgctgggtct gttcccggca gtgagcggtt ttgtacaaca cattccagaa ccggttctgg
    17701 gcggcgcaac gcttgtaatg tttggcacca tcgccgcctc cggtgtgcgt atcgtttctc
    17761 gtgagccgct gaaccgtcgg gcgattctga ttatcgcgct gtcgctggcg gttggtctgg
    17821 gcgtgtctca gcagccgctg attttgcagt ttgcccctga atggctgaaa aacctgctct
    17881 cctccgggat cgccgcgggc ggtattactg ccatcgtgct gaatctgatt ttcccaccag
    17941 aaaaacagta attcactggc ggcggtaaga taattaccgc cgcagtctca cctctttaac
    18001 cattcccgcc ttgaggattg cgcgtaaatc gtgcataact cccttatgtg catttcacgg
    18061 gatggaagac catgaaattt attgggaagc tgcttctcta cattctcatc gctctgttag
    18121 tggcgatcgc tggcctctat tttcttctgc aaacccgctg gggagcagaa catatcagcg
    18181 catgggtttc cgagaatagc gactatcatc tggccttcgg ggcgatggtt caccgttttt
    18241 ccgcgccatc tcatatcgtg ctggagaatg tcacgtttgg tcgtgatggc cagcccgcga
    18301 ccctggtggc caaaagtgtc gacattgcgc taagtagtcg gcaactgacc gaaccacgcc
    18361 atgtcgatac catcctgctg gaaaacggga cgctgaatct caccgaccag accgcgccgc
    18421 taccgttcaa agccgatcgt ctgcaactgc gtgatatggc gtttaatagc ccgaatagcg
    18481 aatggaaact gagcgcgcag cgggtaaatg gcggcgtggt tccgtggtca ccagaagccg
    18541 gtaaagtgct gggtacgaag gcgcagattc agtttagtgc cggatcgctt tcgctcaatg
    18601 atgttcctgc caccaatgta ctgattgaag gcagtattga taacgatcgc gttacgctga
    18661 ctaacctggg tgccgacatc gcgcgcggga cattaaccgg aaacgcgcag cgtaacgccg
    18721 acggcagctg gcaagtggaa aacctgcgca tggcggatat ccgtctacaa agcgaaaaat
    18781 cgctaaccga cttctttgcg ccattacgct ctgtcccgtc gttgcagatt ggtcgcctgg
    18841 aagtgatcga tgctcgtttg caaggtccgg actgggcggt gaccgacctc gatctcagct
    18901 tgcgcaacat gaccttcagt aaagatgact ggcagacaca agaaggcaaa ctgtcgatga
    18961 acgctagcga gttcatttat ggttcgctgc atttatttga cccgattata aacgcggaat
    19021 tttctccgca gggcgtagcg ctgcgccagt tcaccagccg ctgggaaggg ggtatggtca
    19081 gaacgtcagg gaactggctg cgtgacggga aaacgttgat ccttgatgat gcggcaattg
    19141 ccggactgga atataccttg ccgaaaaact ggcaacagtt gtggatggaa acgacacccg
    19201 gttggttaaa cagcctgcaa ctgaagagat ttagcgccag ccgcaatctg atcattgata
    19261 tcgaccctga cttcccgtgg cagctcaccg cgctcgatgg ttacggtgcc aacctgacgc
    19321 tggttaccga tcataaatgg ggcgtctgga gtggctcggc gaatctgaat gccgccgccg
    19381 cgacgtttaa tcgtgttgat gtccgtcgtc cgtcgctggc gctaaccgcc aacagcagca
    19441 cggtgaatat cagcgaactg agtgcattta ctgaaaaagg cattctggaa gccaccgcca
    19501 gtgtttcaca aacgccacaa cgtcagacac atatcagcct gaatggacgc ggtgtgccgg
    19561 tgaatatttt gcaacagtgg ggatggcctg aattaccgtt gactggcgac ggcaatattc
    19621 agcttaccgc cagtggcgat attcaggcca atgtcccgtt gaaacctacg gttagcgggc
    19681 aattgcatgc cgtgaacgcc gcaaagcacc aagtgactca aaccatgaat gcgggcgtcg
    19741 tttccagcgg tgaagttaca tcggcagagc tggtgcagta aacatcaatc tcgccgggta
    19801 cgactcagac gtacccggca ttcttcaata gattacaacg taattgtcag cgcattccct
    19861 tgaggcgtaa ccaccagccc ctgctcactt tcagcctgcg aaccgccttg cagaccattt
    19921 actttcacaa tattgcgcaa gcacagtgtc cagttcctcg cctcgcccgc accagtcaca
    19981 gtaatcgtgt taccagtacg tgttgctttt agagtaaaga tcaccgaacc gtcagcagcg
    20041 ggcacttcac atacagcttc atgcccgtct tgcagattaa agagatggaa tgccgtgcct
    20101 tcgtgccacg cgtaatcggg acgttgatcg ttgttgccca gcgccaatag ggtgttatca
    20161 cgcacataaa cgggcagact caggaagccg tgctgctgtt tatgccagcg actaccgtcg
    20221 agctcattgt tgtgccacag gtgtgtccag cgaccttccg gcaagtagaa ctgcacctcg
    20281 cccgcctcag tgaacaccgg agcaaccatc acgttgtcgc ctagcatgta ttgacggtca
    20341 aggtaatcac aagccggatc gtccgggaac tccatcatca tggcccgcat catcggcgta
    20401 ccccgcgctt tcgcacgcgc agcttcacga tacagatacg gcatcatgcg gcatttcagt
    20461 tgcgtgaaga agcgcaccac atcacaggac tcatcatcgt aggcccacgg cacacgataa
    20521 gatttgctgc catgtaaacg actatggctg gagagcaaac caaacgcgca ccagcgtttg
    20581 taaacgtgtg ccggcgcggt attttcaaag ccgccgatat cgtggctcca gaagccaaaa
    20641 cctgaaaggc caatagacaa accaccgcgc aggctttccg ccattgattc gtagttagcg
    20701 taacaatcgc caccccagtg taccgggaat ttctgcgcac cgacggaggc cgagcgggca
    20761 aacaagacag cttcttcctc accaacggtg tccttgagca cgttccacac cagttcgttg
    20821 tagatgtacg cataatggtt atgcattttc tgcggatcgg aaccgtcaaa ccactgaaca
    20881 tcagttggga tacgttcgcc aaagtcggtc ttaaagcaat caacgcccat cgcgaccaga
    20941 cctttcagtt tgtcggcgta ccatttgcag gcatccggat tggtaaagtc ataaatcgcc
    21001 agacc
//
LOCUS       DAESHI010000071        20425 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.072, whole genome shotgun sequence.
ACCESSION   DAESHI010000071 DAESHI010000000
VERSION     DAESHI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20425
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.072"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            210..422
                     /gene="cspG"
                     /locus_tag="KW011_002700"
     CDS             210..422
                     /gene="cspG"
                     /locus_tag="KW011_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HBH7661000.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            433..621
                     /locus_tag="KW011_002701"
     CDS             433..621
                     /locus_tag="KW011_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBH7661001.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            596..826
                     /gene="ymcE"
                     /locus_tag="KW011_002702"
     CDS             596..826
                     /gene="ymcE"
                     /locus_tag="KW011_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HBH7661002.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            816..989
                     /locus_tag="KW011_002703"
     CDS             816..989
                     /locus_tag="KW011_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HBH7661003.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1038..2111)
                     /locus_tag="KW011_002704"
                     /pseudo
     CDS             complement(1038..2111)
                     /locus_tag="KW011_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(2183..4927)
                     /gene="torS"
                     /locus_tag="KW011_002705"
     CDS             complement(2183..4927)
                     /gene="torS"
                     /locus_tag="KW011_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HBH7661004.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFATQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5010..6038
                     /gene="torT"
                     /locus_tag="KW011_002706"
     CDS             5010..6038
                     /gene="torT"
                     /locus_tag="KW011_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HBH7661005.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6011..6703)
                     /gene="torR"
                     /locus_tag="KW011_002707"
     CDS             complement(6011..6703)
                     /gene="torR"
                     /locus_tag="KW011_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HBH7661006.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6823..7995
                     /gene="torC"
                     /locus_tag="KW011_002708"
     CDS             6823..7995
                     /gene="torC"
                     /locus_tag="KW011_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HBH7661007.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNESDTAGKAHGDKKDEK"
     gene            7995..10406
                     /gene="torA"
                     /locus_tag="KW011_002709"
                     /pseudo
     CDS             7995..10406
                     /gene="torA"
                     /locus_tag="KW011_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="KW011_002710"
     CDS             complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="KW011_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HBH7661008.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="KW011_002711"
     CDS             complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="KW011_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HBH7661009.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            12531..13677
                     /locus_tag="KW011_002712"
                     /pseudo
     CDS             12531..13677
                     /locus_tag="KW011_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13971..15212
                     /gene="agp"
                     /locus_tag="KW011_002713"
     CDS             13971..15212
                     /gene="agp"
                     /locus_tag="KW011_002713"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HBH7661010.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(15250..15477)
                     /locus_tag="KW011_002714"
     CDS             complement(15250..15477)
                     /locus_tag="KW011_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HBH7661011.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(15498..16094)
                     /gene="wrbA"
                     /locus_tag="KW011_002715"
     CDS             complement(15498..16094)
                     /gene="wrbA"
                     /locus_tag="KW011_002715"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBH7661012.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            16467..16640
                     /locus_tag="KW011_002716"
     CDS             16467..16640
                     /locus_tag="KW011_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HBH7661013.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(16898..18226)
                     /gene="rutG"
                     /locus_tag="KW011_002717"
     CDS             complement(16898..18226)
                     /gene="rutG"
                     /locus_tag="KW011_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HBH7661014.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(18247..18741)
                     /gene="rutF"
                     /locus_tag="KW011_002718"
     CDS             complement(18247..18741)
                     /gene="rutF"
                     /locus_tag="KW011_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HBH7661015.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(18752..19342)
                     /locus_tag="KW011_002719"
     CDS             complement(18752..19342)
                     /locus_tag="KW011_002719"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HBH7661016.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(19352..20152)
                     /gene="rutD"
                     /locus_tag="KW011_002720"
     CDS             complement(19352..20152)
                     /gene="rutD"
                     /locus_tag="KW011_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HBH7661017.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPC
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(20160..>20425)
                     /locus_tag="KW011_002721"
     CDS             complement(20160..>20425)
                     /locus_tag="KW011_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HBH7661018.1"
                     /translation="VLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKN
                     YAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
BASE COUNT         5105 a         5199 c         5243 g         4878 t
ORIGIN      
        1 ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc tgcgtcggtt
       61 tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc aggcattatc
      121 cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag ccctgtttgc
      181 cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt ttagtaaaat
      241 ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc aaagacgttt
      301 tcgtccattt caccgccatc cagagcaatg aattccgcac gctgaacgaa aatcagaaag
      361 ttgaattttc tattgagcag gggcaacgtg gccccgcggc agcgaacgtt gttacgctct
      421 aaggttgccg ttattactca acatctccat ttccgctgtc catgttgtca tggttcacag
      481 taccgcacat cggcattcga tgtgacggag cgaaaccctt tgggcgctaa gtgtattttt
      541 tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc aaatacgcac tgaccatgcg
      601 ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta tatctgcctt
      661 atttttcttc aacgctgtat gtatagtaag cgataacctg ttgattattg aatctttcgg
      721 ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca ccttactggc
      781 gtgttgctgt cttttaagac cagaagaggt taacagtgaa tattgaagag ttaaaaaaac
      841 aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg aacaagaata
      901 cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc gggcttgaaa
      961 gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg
     1021 ttccgcttgt tgctaattta gtgaccaatc attggcgctt gtggaattaa gcgtcggtac
     1081 aattcctccg gcaccgggct ttgccatact cccgcataca ttgcgtaacc aatcaccgcg
     1141 aacataatcc tcagaaccag tagcgtcatt aaccagccag acaacgcaaa agctttttta
     1201 tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg ctaccggaca tgactctacg
     1261 caagtcatac agccggtaca ttccactgtt cgtacctgaa ttaatttatc taccgggatc
     1321 cgtgatgggc aattttttgc gcatttgcca cagccgatac aactttcggc attgcgacga
     1381 atcttaaacg gcgataatag cgaaaccacg cccatcagcg cgccatatgg gcaaagataa
     1441 cgacactagg catggcgaat aaacaggctg gcaatcagca aaacggtcac gctgattaat
     1501 gtcgcggtcc ccatatgacg aaagaaatcg agcattttta cgtccatcac cacgctgtag
     1561 ggcgacaaca taaaatagtg aatcgcctga gcgggcatca ataacgcgat atagagaaaa
     1621 aaactcaaca gcaaatactt cacgccgcgc agaggaatat ccagccagcg gggaaggaca
     1681 cattgccgac caaacagttt gttaccgaga tcgccgatta attcagaaag cgtaccaacc
     1741 gggcataacc atgagcaaaa ggcctttttg agtaatagac tgatgacgat aaaagcgacc
     1801 aataacagca tcgcggcggc gtggacggac ggtaactgac ctgttgtaag gctatatttc
     1861 agattcatca gaccggcaat cggtagccag ccttcgatac ctcccggtct ggcgacaaat
     1921 gtcgtgctac ttgccgtttc gtaatagcgc acccaatacc agaacgtgat ggcaatataa
     1981 atattcattg ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc attgcgccag
     2041 tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt
     2101 ttctctgcca tgattttgcc agtccgttaa gttgcatacc aaatgccact attctagttg
     2161 ttcttaactg gctgatattg attcaaatcg cgttcaggtc tttcttatgc aaccatgctt
     2221 ccagagcggc aacactgcgt gtaatttctt cgtgtggaag gggggcagat aatggctgct
     2281 gctccagttg tgcgcatagc tggctggcga tacgcattcc caaactcgag caactgcttt
     2341 ttagctgatg cgcggcacgc tttattttct cgctatcctg actggcgcgg gcaatgtcga
     2401 tttcatcgag aagcggcagg gcatgttgtg taaataatac cagccattcg tggatcttct
     2461 ccgtccccat taactgagca tcttcattga gttgcgatac atccagcgat tgatcgttat
     2521 tgacttgcag ttggagatag tgcgccagta actgaccgag cacttcacgc ggcaccggtt
     2581 tagggataat cccgcggaat aatgaactgg tacgctggcg cagcgtttcg tcaatgacat
     2641 gggcgctaaa gccaatcaaa accagcgacg gatattgctg tgccagttgt cgggcaagcg
     2701 taatgccgtc gacatccggc agatcaaaat ccaccagtgc ggcagcaaac ggttcgctat
     2761 tttgcagtgt ctctaaagcc tgcgcggcat tgccaacagc aacaacctgc gcaccactgg
     2821 tgttcagcat ctcaacggta attcgctggg ttagcgggtt atcttcaatt aacagcaaac
     2881 gtaaaccgtc aagacgcacc gcctgattga ctgtttttgg cacgggtgcc gtggcaacac
     2941 gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg cgtgctggtg gcgctcagtt
     3001 cgccgcccat cgcctgggcc agacggctac tgatagtcag ccccagcccg gtgccgccgc
     3061 gtttgccgct tacctgcaca aatggctgga agatttctgc cagtttcgcg ggatcaatac
     3121 cgcagccgct gtcttccact tcgaccagcc attgctcgcc atcagtgcga ctacgcagga
     3181 taatgtaccc ttcgtcagta aaacgcaggg cgttgctcaa caggttggtt ataacctgac
     3241 gaatacgtcg tggatcgccc attaacgcgc acggcatatc atcggcaatt gccgttgcca
     3301 ggcgaatcgg gcgacctttc acccgtccgc tcattaattg cagggtactt tccagcagcg
     3361 ggcgcggttc aaagggctca tcgctgaccg aaacattctt gccacctgct tcgatagcgg
     3421 aataatcgag aatatcgttg aggatggtca gcaacgattc accagagtca gtaattgccc
     3481 gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc cagtagctgt gcggtgccga
     3541 gaataccgta cagcggtgtg cggatctcat ggctcatcgc cgccagaaac gccgattttg
     3601 cctggctggc tttttctgct tccgcccgtg cctgtcggtg ttctatcact agttcctgca
     3661 attcagctgt acgcgctttg acctgcgccg ccagctgttc acggtggcga ttcagtgcat
     3721 gaacactgct gcgaaacgca cccatcagct gcccgatggt gtccagctcc cgtacgccag
     3781 cggtttccgg gaaaggggag tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt
     3841 gttcggcaag tggacgcgtg actgagcgat aaaccacgcg ccagaggatc agaatcagtg
     3901 cgcaaagtga aaccatcccc agcaataaca ggctgtattg cccgcgtgca ctggcttttt
     3961 cctgatgcgc cagtccgtgc tgattacgca gctcaatggt gtcgaccagc tgactgactt
     4021 cgctactaaa ctgcgcgaac tgggcgatat tattttgggc gagagtttgc aggtgattgc
     4081 tgatttcact gtcctgctgg aacagcgcca acagatcgct atattggcta acggtagtta
     4141 acgttgttgc gacctgcgca cgaactcccg gatcttcaat gcgtatttgc cgacgttgca
     4201 gaattttcac cgcattattg agctgctttt ccagcgttgg tgcatttttc tggatctgct
     4261 ccagccccag attcatcacc atttgctgca cccgcagagc gctaaggcgc agttcattca
     4321 tctggttaac atactcaaga tcgatatcaa tcagccgatc gagtgcactt tcagcagcct
     4381 gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg
     4441 tcgcattatt cgcctgacct tgcgccaggc gtgcgatctc atcggcggca gcgactatct
     4501 gctgactgag ttgctgttgt tgctggcgta gttgcagacg ccgccccacc agttcccctt
     4561 gctgacgtaa cgaacgggag atctcctgct cctgttgttc aatagcggtg gtatcaaaac
     4621 cttgttcccg taacgcttgc agcaacgcat taatcttcag gctttgtgcg gtgagcattc
     4681 gcccctgcgc ctgccacatc ttttcgttat cggcactggt caggttctgc gtggcgaaaa
     4741 gttcccaggc gctggcttcg ctcaactggc gcgccatatt catggtagga atcaatgcct
     4801 gagtattgtc tttttccacc tggctgataa agcgcaggtt gtaccatccc accagggtac
     4861 tggtcagggt taacagcgcc atcagggcaa agcccatcca gagtcttcgg gtcagggtta
     4921 aattcacggt cggtgcactt taggtgaaaa atgttgagtc gcaaagcgga atgcatctag
     4981 cataaagcct tattattgat gaggctatca tgcgcgtact gctattttta cttctttccc
     5041 ttttcatgtt gccggcattt tcggctgata acctgttgcg ctggcatgat gcgcagcatt
     5101 tcacggtgca agcctctatg ccgcttaaag ccaaacgcgc atggaaactg tgcgcgcttt
     5161 atcccagcct gaaagattca tactggttat cgttgaacta tggtatgcag gaggcagctc
     5221 gccgttatgg tgtggattta aaagtgctgg aggcaggcgg ctacagccag ttggctaccc
     5281 agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga ggccatttta ctcggtagta
     5341 gcacgacctc atttcccgat ctgcaaaagc aggtagcaag tctgccggtg atcgaactgg
     5401 taaatgctat tgatgctccc caggtgaaaa gccgcgttgg tgtaccctgg tttcagatgg
     5461 gctatcaacc tgggcgatat ttggtgcaat gggcgcacgg taaaccactg aacgtgctgt
     5521 tgatgcccgg acccgataat gccgggggca gtaaagagat ggtcgagggt tttcgcgcag
     5581 ccattgccgg aagcccggtg cgtattgttg atattgcgct tggtgataac gatattgaaa
     5641 tccagcgtaa cctgttgcag gagatgctgg aacgccaccc agaaatcgac gtcgttgccg
     5701 gaacggccat tgcggcagag gcggcaatgg gtgaagggcg taacctgaaa acgccgctta
     5761 ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg gctgaagcgg ggaagagtga
     5821 ttatggctgc cagcgatcaa atggtctggc agggggaact ggcggttgag caggccatca
     5881 ggcaattaca ggggcaatcg gtgtctgata atgtcagccc accgatttta gttctgacgc
     5941 cgaaaaatgc cgaccgtgaa catattcgcc gctcgctgtc accaggggga tttcgtccgg
     6001 tctattttta tcagcacaca tcagcggcta agaaataacc ttcaccatgt tgcgtcacca
     6061 gtaaatccgc gctgagttta tgacgtaaac gacgaattaa cacatcgacg gtgcgcaggt
     6121 cagggttttc cacccgacgc gcagaaagca tacgtagcag acgttcacgg ctgagaattt
     6181 cgcccggatt cgtcacaaat gccaccaaca tttcatactc tgcgcgggtc agtttaatcg
     6241 gctcgccatc ccgctccagc gtatggcgcg acacattcag gcaataaccg gcaaagcgat
     6301 agcagttgtc ctgagtgtgc ggttgagctt gtcgcgcgag gtcgattcgc cagagcagat
     6361 ttttcacccg tactaccagt tcgcgcagtt ccagcggttt ggtgacgtaa tcgtctgcgc
     6421 ccatttccag cccaacaata cggtcaatcc gatcgctgcg tccggtaacc agaataatcc
     6481 ccaccgttga gcgttctcgc agggcgcggg ttaacatcag gccattttca tcgggtaagt
     6541 tgatatccag cagaattaaa tctaccggct gattctgcat aatttcccgc agcccggcac
     6601 cgctcgctgt aacggaaacg gtatacccct cctgagtgaa gtaagattgt aatcgcgcct
     6661 gggtaaccgg ctcatcttca acaataacaa tgtgatgtgg catcagaggg ttttactcat
     6721 tctgttcata ttctgccgta agccgttcat cctgaccagt gccgctgttc atatttgctc
     6781 attaagatcg cttcactaaa ccataatttt acaggggcta ttatgcggaa actctggaac
     6841 gcgctacgcc gacccagtgc tcgttggtcg gtactggcgc tggtcgctat tgggattgtg
     6901 attggcattg cgctgattgt attgccacac gttgggatca aagtcaccag cacaaccgaa
     6961 ttttgtgtca gttgccacag tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat
     7021 tttcagaacg cctctggcgt gcgagctgaa tgccatgact gtcatatccc gccggatatt
     7081 ccaggcatgg tgaagcgcaa actggaagcg agcaacgaca tttaccagac ctttattgcc
     7141 cactccattg atacacctga aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa
     7201 tgggcgcgaa tgaaagaaaa caactcggca acctgtcgct cctgccataa ctacgatgcg
     7261 atggatcatg ccaggcagca tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat
     7321 aatcaatcct gcatcgactg tcataaaggt attgcccacc agttaccgga tatgagtagc
     7381 ggcttccgta agcagttcga tgagctgcgc gccagtgcta atgacagtgg tgacacgctg
     7441 tactctattg atattaagcc aatttatgcg gcgaaaggcg ataaagaagc ctctggttct
     7501 ctgctgcctg cttcggaagt gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa
     7561 attaccggct ggacggaaag cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa
     7621 cgcatctttg ttgcctcgat tcgtggtgat gtgcagcagc aggtgaaaac gctggagaaa
     7681 accaccgttg ccgacaccaa taccgagtgg agcaagttgc aggccaccgc gtggatgaag
     7741 aaaggcgaca tggtgaacga tatcaaaccg atctgggcct atgcggattc gttgtacaac
     7801 ggcacctgta accagtgcca cggcgcaccg gaaatcgccc actttgacgc taacggttgg
     7861 atcggcacgc tcaacggcat gattggcttt accagcctcg ataaacgtga agaacgcacc
     7921 ttgttgaaat atctgcaaat gaatgagtct gacaccgcag gtaaggctca cggcgataag
     7981 aaggacgaaa aataatgaac aataacgatc tctttcaggc atcacgtcgg cgttttctgg
     8041 cacaactcgg cggcttaacc gtcgccggga tgctggggcc gtcattgtta acgccgcgcc
     8101 gtgcgactgc ggcgcaagcg gcgactgagg ctgtcatctc gaaagagggc attcttaccg
     8161 ggtcgcactg gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac
     8221 cgttcgaact ggataaatat ccgtcgaaaa tgattgccgg attgccggat cacgtacaca
     8281 acgcggcgcg tattcgttat ccgatggtac gcgtggactg gctgcgtaag cgccatctca
     8341 gcgatacctc ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg
     8401 acatgttcta tgaagaactg gaacgcgtac agaaaactca cgggccgagt gccttgctga
     8461 ccgccagtgg ttggcaatcg acggggatgt tccataacgc ttcggggatg ctggcgaaag
     8521 ctattgcctt gcatggtaat agcgttggta cgggcggaga ttactctacc ggtgctgcgc
     8581 aggtgatcct gccgcgcgtg gttggctcaa tggaagtgta tgaacagcaa acctcctggc
     8641 cgctggtatt gcagaacagc aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc
     8701 agcaagcgaa ctggtggtgc ccggatcacg atgtttatga atattacgcg cagctaaaag
     8761 cgaaagtcgc cgccggtgaa attgaggtca tcagcatcga tccggttgtc acatccaccc
     8821 atgagtatct ggggcgcgag catgtgaagc acattgcggt taacccgcaa actgatgtgc
     8881 cgctgcaact ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc
     8941 tcgctaacta ctgtgtgggt tttgagcagt tcctgccgta cctgctgggt gagaaagacg
     9001 gtcagccgaa agatgccgca taggctgaaa aactgaccgg cattgatgcc gaaaccattc
     9061 gtgggctggc gcggcagatg gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc
     9121 agcgtatgca gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg
     9181 ggcaaattgg cctgccaggt ggtggctttg gttttggctg gcactataac ggcgcaggca
     9241 cgccggggcg taaaggcgtt attctgagtg gtttctccgg ctctacgtcg attccgcctg
     9301 ttcacgacaa cagtgattac aaaggctaca gcagcaccat tccgattgcc cgttttatcg
     9361 atgcgatcct cgaaccgggg aaagtgatca actggaacgg taaatcggta aaactgccgc
     9421 cgctgaaaat gtgtattttt gctggaacta acccattcca tcgccatcag cagatcaacc
     9481 gcattattga aggctggcgc aagctggaaa cggttatcgc catagataac cagtggacct
     9541 caacctgccg ctttgccgat atcgtgctgc ctgcgaccac gcagtttgag tgtaacgatc
     9601 tcgaccagta cggcaaccac tccaaccgtg gcattatcgc catgaaacag gtggtgccgc
     9661 cgcagttcga ggcgcgcaac gactttgata ttttccgcga gctgtgccgt cgctttaatc
     9721 gcgaagaagc ctttaccgaa gggctggacg aaatgggctg gctgaaacgc atctggcagg
     9781 aaggtgtaca acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat gacttctgga
     9841 ataacaaaga gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac caggcattcc
     9901 gcgaagatcc ggatctcgaa ccgctgggca cgccgagtgg cctgattgag atctactcga
     9961 aaaccatcgc cgatatgaac tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa
    10021 tcgaacgctc ccacggtggg cccggctcgc agaagtatcc gttgcatctg caatctgtgc
    10081 atccggattt ccgacttcac tcgcagttat gtgagtcgga aactctgcgt cagcaatata
    10141 cggtagcggg taaagagcca gtattcatta acccgcagga tgccagcgcg cgcggtattc
    10201 gtaacggtga tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca ggggcagtgg
    10261 tttctgaccg ctatgcaccc ggcgtggcac gaattcacga aggggcatgg tacgatccag
    10321 ataaaggcgg cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg ttgaccatcg
    10381 acatcggtac atcgcagctg gcgcagttgt tctcccgtga actggacgat gaacaactga
    10441 cgcaaatctc cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc
    10501 tcactgcggc ggtgaatgcg ctggaaaacc gtattgccgc tctgacagta cgtgacgatg
    10561 cccgtctgga actggccgct gacttttgcg gcctgtttct gatgaccgac aaacaagcgg
    10621 cgctgccgta tgcatcggcc tacaaacagg acgagcaaga gattaaacgc ttgttagttg
    10681 aggcagggat ggaaaccagt ggcaatttca acgaatcggc agatcatctg gcgatctatc
    10741 tcgagttgtt gagtcatctg catttttcgc tgggagaggg gaccgttcct gcgcgaagaa
    10801 tcgacagttt gcggcaaaaa acactgacgg cgctgcggca atggttacca gagtttgctg
    10861 cgcgttgtcg acagtatgac agctttggtt tttacgcggc actaagccag ttattgctgg
    10921 tgttagtgga gtgcgaccac caaaacagat aacgtcgttt atgcgcctga aaagacgcgt
    10981 ttagcgtcgc atcaggcatt atggcgcagt tgccggatgc gtcatcacat ccggcaaaca
    11041 cacatcacgg atgagctaca aaccgggaaa gccgctggcg tagcaggcgg ttttcctgct
    11101 tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatccccggc cagtccagag
    11161 ccagttcatg acgcaggcgt accgcgcgtt gcaccacaat ggcggcatgg tcgtcaaata
    11221 cccaggttgt ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattca
    11281 actcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa
    11341 cattagccat catgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc
    11401 cagttgctgc cacagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag
    11461 taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa
    11521 tcgttgcccg gcctggctgc ctggcgggat agtcagcaaa atgctttctt tcagtgttgg
    11581 aacggtgact ttagcaccca gcgccgcttc ccacgggcta accggcacca caatttccag
    11641 atcctggccg acaatatcga acagcggatg tggcgcaata tgaatcacca gccacaaatc
    11701 gccatttgga ccaccgtttt cgcccggcgt cccctggcct ttcagacgga tgcgttgacc
    11761 attgccgacg cccgccggga tcttcacatt cagcgttttc gggatttcct gttcgatcat
    11821 gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct cagtcagcgt
    11881 ttcttcgagg aataccgcca cttcgatttc aatatcgtgg ccgcgtgcgg cggggcgttg
    11941 acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc
    12001 attaaaactc tgaccgtcgc catgatggaa ctgatggtta aattgcggat cgttgcgatg
    12061 ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc
    12121 agcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt
    12181 acgggccagt cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg gtttcacgcc
    12241 catgatggcg taataatcct ttaattccat agcgttatct cgcgtaaatc aacacaaatt
    12301 gaaggaaccc ctgtaaggta actcctataa gtgtagggta atcctcaaaa tttcatatgc
    12361 caacacagaa tatgttattg aaatcatcgc ggagaggagg tcgccatcaa gatgggttgc
    12421 tgaacatatt ttaaacaggt gaaaaagggt gagcgatttt tgatagttga accaggcact
    12481 ttaagtttaa ctagggcgtc attatttatt aaattttata gacgctatat atgggtagta
    12541 atatacatgg aattagttgc actgcaaata attatttgaa acaggcctgg aacgatataa
    12601 aaaatgagta cgaaaaaaat caaacatatt caatcacgct ttttgaaaac acactggtgt
    12661 gttttatgcg gttatacaat gaactcagac gtaaagtaaa tgaagaggat actccatgtc
    12721 tggaatgtga atcactagaa aaagaatttg aggaaatgca gaatgataat gatctatcat
    12781 tatttatgag aacattgcgt actaatgata cacaaattta ttcaggggtt tcaggaggta
    12841 ttacatatac tatacaatat gttcaaaatg ttgatattgt tagagtgtcc ttgccgggca
    12901 gaggttcaga gtctatcaca gattttaaag gttattattg gtatggcttt atggaatata
    12961 ttgaaaacat tactgcgtgt gatgatattt tttctgagta ttgtttagat gatgaaaata
    13021 tgagtatcca gccagaacag ataaatacgc cgggaatatc tgatttagat actggtttta
    13081 aaagaacatg agtattcatg ggcgctgggc ggccataaga cgcaaaagca agtatacgaa
    13141 gagtttatta aaattgttga taaacaaacg atcgaggctc ttgttgatac ggcattctat
    13201 aatctcaggg aagatgtaaa gacgttattt ggcgttaatc tacaatctga caacaaaatt
    13261 caggggcagg gtggtttttt atcatggagt tttttgtctt ttaaaaaaga atttattgat
    13321 agttgcttga atgacaaaaa atgcatactg catttacccg aatttatatt taatgataac
    13381 aaggcacgcc ttgttttaga caccgaccca gagcagaagg ttaatgaggt gaaaaatttc
    13441 ctcactgctc tttcagatag catttgctca ttatttattg tttacagcaa tgtggcatca
    13501 atctccttag ggaatgaatc cttttcaaca gatgacgacc ttgagtatgg gtatttaata
    13561 aacactggca atcattatga cgtttacctc cctcctgaac tttttgctca ggcttacgag
    13621 ttaaacaata aggaaaggaa tgcgcaaatt gattttttaa ctcgttatgc aatttaatga
    13681 caaaggcatg tgctaaaaac cattgttatt agcctcactt ctcttattat tggtactatt
    13741 gcctctgtat tagtaacgcc gctgatattc tgtttagcca caggtgcaat tatcagcggc
    13801 gtacgcgagg caggggctaa tcaggcatag tttgcgtcaa accttgcctg tttttgaaga
    13861 tatatataga aaaacaggcg ttcaacaagc cattttgcga acctgttccc ggaaaaaagt
    13921 catatttctg tcacactctt taatgattga taacaaaaga ggtgccagga atgaacaaaa
    13981 cgctaatcgc cgcaactgtg gcagggatag ttttactcgc ttcaaatgct caggcacaaa
    14041 ccgtaccgga aggctatcag ctacagcaag tgctcatgat gagccgccat aacttacgtg
    14101 cgccgctggc gaacaatggc agtgtgctgg agcagtcgac gccgaataaa tggccagaat
    14161 gggacgtccc cggtgggcaa ctcaccacca aaggtggcgt gctcgaagtg tatatgggcc
    14221 attacatgcg tgaatggctg gcagagcagg ggatggtgaa atcgggggaa tgcccgccgc
    14281 cggacaccgt ttatgcctat gccaatagtc tgcaacgtac cgttgcgacc gcacagttct
    14341 ttattaccgg cgcattcccg gggtgtgata ttcctgtgca tcaccaggaa aaaatgggca
    14401 ctatggaccc aacctttaac ccggtgatca ccgatgattc cgccgcattc agtgaacagg
    14461 cggtggcggc aatggagaaa gagctcagca aactccagct taccgacagc taccagctac
    14521 tggaaaaaat cgttaactat aaagattccc ctgcctgtaa agagaaacaa cagtgttcgc
    14581 tggtggatgg caaaaatacc tttagcgcca agtatcaaca agaaccaggt gtttccgggc
    14641 cgctgaaagt cggcaactcg ctggtagatg cgtttacttt gcaatattac gaaggttttc
    14701 cgatggatca ggtggcctgg ggagaaatca aatctgacca gcagtggaag gtgttgtcga
    14761 agcttaaaaa cggctaccag gacagcctgt ttacctcacc ggaagtggcg cgcaatgttg
    14821 cgaaaccgct ggtcagttat atcgacaaag ctctggtcac cgatcgcacc agcgcaccga
    14881 aaattacagt gttggttggg cacgactcca acattgcctc tctgttaacg gcgctggatt
    14941 tcaaaccgta tcagttgcat gaccagaacg aacgcacgcc gattggcggc aaaatcgttt
    15001 tccagcgttg gcatgacagc aaagccaatc gcgatttgat gaaaattgaa tatgtgtatc
    15061 agagtgcgga acagttacgt aatgccgatg cgttaaccct gcaggcacct gctcagcgtg
    15121 tgacgctgga attaagcggt tgcccgatag atgccaacgg tttctgcccg atggataaat
    15181 ttgatagcgt gttgaatgaa gcggtgaaat aacagaaaac tcccccgcga gaagcggggg
    15241 agtcgctggt taaacgtttt tacgttcgat ggtctgttcg ccccaaaaaa gcgaatcttt
    15301 atcggcctta gcaaaggctt tgactaacac ttcatcgcta ccttcttccc aaatcttttc
    15361 tgccattttt tcgtcgtacc cggcgacttc gaaaatggcc tcggctattt ccggcgacgt
    15421 attgcgcaga gatgcccatt caccgacgtg atgagctttc gcttcttgag ttggcatgcg
    15481 tatcctcctg ttgaagatta gccgttaagt ttaactgcca gacctgcgac atattcccct
    15541 tgataacgag caatagacag ttcttcctgg cttggctggc gtgagccgtc accgcctgcg
    15601 atggtggttg cgccgtacgg cgtaccgccg cgaacctgtg aaacgtcaaa taattcctgc
    15661 gctgcgtagc caatggggac aatcaccatg ccgtgatgcg caagggtcgt ccaggttgaa
    15721 gtaatagttt gttcctgacc gccgccagta ccggtggaac taaagacgct cgccagtttt
    15781 ccgtatagtg cgccggaagc ccacaggccg cccgtctggt cgaggaaggt acgcatttga
    15841 ccggacatgt tgccaaagcg ggtaggtgta ccaaaaataa tggcgtcgta atcggccagt
    15901 tcttgcgggg ttgcaaccgg tgcagtttgc gttttaccgc ctgctttttc aaataattgc
    15961 ggcggcatgg tttccggtac acgcttaacg acaacttctg cgccatcgac tttgcttgca
    16021 ccctcagcga ctgcgcgtgc catcgtttca atatgtccgt acatggaata ataaagcacc
    16081 agaactttag ccatttctaa ccactcctcg tgttatctct attccgtagc gattcgctac
    16141 cacttattta aagataagac gtccttttca gagtgcaaat ttcacaacca cttatttgat
    16201 ttataacaac tttcacaagc acgtaatttt gtcgcaaaat gacacatttt tatctcatcg
    16261 cgttttttta atcataagag cggcttatgg ataattattg gagatgatat ctattctcgc
    16321 taagaagctg ttgcaggata ttaccaaacg cgggtctacc cgcgtcagtt cactaagctt
    16381 agtcccacgt agcgaaaata tggcagccgc catacgccgc gttaattcta tgcaatatga
    16441 tgtctatacc cagacggagg tcagtaatgg caaaccatcg aggcggttcc ggcaattttg
    16501 cagaagaccg cgaaagagca tcagaagcag gtaaaaaagg tggacagcac agcgggggta
    16561 atttcaagaa tgacccgcag cgcgcatctg aagcaggtaa aaaaggtggc aagagcagtc
    16621 acggcaaaag cgacaactag ccgggctaat caatgacgaa tgcatttttg tctgtagctc
    16681 gtcaaaaagc catcaccgcc ggttacccgg tggttgatac tgatgacaaa tgtaagcttg
    16741 cctgatgcgc gatgcttatc aggcctacca gaagattgca atatattgaa tttgcactgt
    16801 tttgtaggcc ggataaggcg tttacgccgc atccggcatg aacaatgcgt acgttgtcaa
    16861 caatctgcac cgccgggtaa ccccggcggt tttctgttta tggctcctga tgaacaactt
    16921 ctggcggtgg aacgtcaacc aaccttctac ttaacaacgc attgagtaaa atcgcgccaa
    16981 aggtggctgt accgatccct cccaacgtaa aaccgcccag cgtgagagca aaatcacccg
    17041 cgcccagcac taaggttact gcgaccataa tcaaattacc gttctggctt aaatcgacac
    17101 ggttttgtac ccagatcctt gcgcctgcga cggcaatcag cccgaacaca acaattgatg
    17161 caccaccaat caccgcggcc ggaatggtat ggatcagcgc gccaaatttc ggcgaaaagc
    17221 ccaacagcat ggcgatgacg gcagcagcaa caaacaccag cgtcgagtag acttttgtca
    17281 cggccatcac accgatattt tcagcatagg tggtgacgcc gctaccgccg acagagccgg
    17341 aaagcatcgt tgccagacca tcaccgacga atgctcgccc catatacggg tccatattgc
    17401 gtccggtcat cccggcgacc gccttgagat gacctaagtt ttccgccacc agaatcaccg
    17461 ccacgggcgc aatcagcatc attgcctgac tattaaaagc aggagtggaa aaatgtggca
    17521 aaccgaacca ggcagcatgg ctgacgagag taaaatcgac ggcttttccc agccctaaaa
    17581 cgttggtcat cacgccatac agcagacagg cgacaattaa acctacgaga atcaataacc
    17641 gctggatcat gccacgggta aacaccgcca ccagcccaat acacagcacc gtcattaccg
    17701 ccatccagct atcaaaggcc gaagccgata cacttttcac tgcgataggc gctaagttca
    17761 ggccaatcgc catcaccacc gcacccgtca ccaccggcgg cattagtcgt tcaatccagc
    17821 gtgtaccaat tttcatcacc accaggccaa tgacggtata aaccagccca caggcgataa
    17881 tcccgcccag tgcgatgctg atattcgggt taatgccctg accgttaaag cccgtcgcgg
    17941 caatcaccac gccgacaaaa gccgcgctgg agccgagata actggggacg cgcccgccgg
    18001 taataaagaa aaacagtaac gtgccgatcc ccgacattaa aatggaaaga ttgggatcca
    18061 gccccatcag aatcggcatt aacaccgtcg cgccaaacat cgccaccgcg tgttgaacgc
    18121 ccattactgc cgtctgagca aacggcaatc gttcatccgg cgcgaccacg ccgctctctg
    18181 tagaggtcga ttttaactgc cagtgaggaa aaccgaacat tgccatcagc tgtctcctta
    18241 aggaggttaa caagcagggc gcatcagcgc gtgataactg cgatcgaacc acaccagccc
    18301 gtagggtgtg gtgtgacgat gaatcgcttc gatggcgcaa aacagaatgt cgtgggtgcc
    18361 gacgctcacc acctggctga tgcggcagtc aaacgaaacc agagcctctt ccagttgcgg
    18421 gcatccggtc acacccgtct gccagcgggc ggcggcaaag cggtgttcca tgggcgtttt
    18481 gccgccaaaa agatttgaaa gcggctcctg cccggcgctc agtgtattta cacacagcgt
    18541 tcgattttca ttgaataccg gccagacgga cgccccacga ttcaggcaca ccagtaatgt
    18601 gggcggcgtg tcggtcacgc tgcagacggc gctggcggtg aacccggcgc gcccggatgg
    18661 accgtccgtg gtgatgatat tgaccgccgc gcccatacag gacatcgcat cgcgaaaggt
    18721 ttgttgatca actatgttca tagtttgctc cttacaacag cccgcaggct tcttcaaagg
    18781 acagacgtgg caggcgcgca aaaagtttgc tgctatcgcc atagccgata ttaatcagca
    18841 gattgctctt cagcgtgctg cccgcaaaaa aggcgtcgtc aacgtgttga cggtcaaaac
    18901 ccgacatcgg gccggtatcc agtcccagcg cacggcaagc gacgatcaga taggccgcct
    18961 gcatggagct gttgcgaaac gctgtttctt cggcaagttg tgggctggag gtaaaccaac
    19021 tgcgggcatc accgtgggga aacagtagtg gtaaccgttc ataaaattca ctgtcccagg
    19081 caacgatagc ggtgacgggc gcggtcaggg ttttttgtag attgccgctg gaaagcgccg
    19141 ggtgcagacg ttcttttcct tctacagtgc ggataaacac gatccgtgcc ggagaacagt
    19201 tagctgatgt cggcccccat tttatcgggg cataaagctc ccgtaacgtc tcatcgctga
    19261 cgggtgtctc ccgccagccg ttgtgagtgc gggcatcggt gaacagggtg ctaagcgcac
    19321 ctgggctaac ggcttcgttc atagcaattc cttacagggc ggcttcacgg tgatgtaaca
    19381 ggctggcaag cccgttgagt aacagagcat taaacgtttc ggggtcggtc acgttgcagg
    19441 cgtgtccgcc atagcgcatc accattttct ggctatcggg cagggcggca tgaagctcac
    19501 tggaacatgc tgatggcacc agcagatcat cactggcgca gatgatttgc accgggcagc
    19561 ggatgcgatc cgcatggtga ctaaagtcag cgcgtttgag ggcgttaagt cgacgcagta
    19621 aattattttt gccctgaaaa tgcgccagtg ccagcgcgtc ttctgcctca aggcaaggtg
    19681 cgcgggccgc catccagtcg gcgggataga ggaacaacgg ctgcgcttcc acccatgcct
    19741 gcgcgccgcc gctatacagt aatcgttcgc gaacctgaaa acagcggcgc gtatgggcgt
    19801 ttattcgtag ccagccgtta acgctgatca gcacagttac cgacgcggga taatccagcg
    19861 ccagctgcat tcccaccagc gcaccgagcg catggccgac caccgcgtaa tgctcaatcc
    19921 ctgcggctac cagcgcctga tgcagttccg ctgccatctg ggcgatactg taatcttctg
    19981 ccagcgtgtc gggattattg ccggtgccgc gctggtcgta acagactacc tgatactcct
    20041 gctccagcac cgccagttgc ggtaaccagt aactgccgct accgccaaga cccgaaatca
    20101 acaccactac gggcgcatca gcataagggg gaggtgagag tgaaagtttc atcgcggcct
    20161 cacttggcga tatgcgcaat tgtggcgatt tccaccagcg cgtcaggttt taccagtccg
    20221 cactgaatgc agaatcgcgc cggtttatca cccggaaaaa actcggcgta gatttcgtta
    20281 atcgcggcgt aatttttcca gtcggtaata aagatgctgt tgaaggtcac atccgccatc
    20341 gtgccacccg ccgtctcgat caccttgcgg atagtttcca gaacgtggcg ggtttgcgcc
    20401 tttgggtcat cggcaaacag cacgt
//
LOCUS       DAESHI010000072        20240 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.073, whole genome shotgun sequence.
ACCESSION   DAESHI010000072 DAESHI010000000
VERSION     DAESHI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20240
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.073"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            689..1459
                     /locus_tag="KW011_002722"
     CDS             689..1459
                     /locus_tag="KW011_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HBH7661019.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1450..1923
                     /locus_tag="KW011_002723"
     CDS             1450..1923
                     /locus_tag="KW011_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HBH7661020.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            2031..2570
                     /gene="dnaT"
                     /locus_tag="KW011_002724"
     CDS             2031..2570
                     /gene="dnaT"
                     /locus_tag="KW011_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HBH7661021.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2573..3310
                     /gene="dnaC"
                     /locus_tag="KW011_002725"
     CDS             2573..3310
                     /gene="dnaC"
                     /locus_tag="KW011_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HBH7661022.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3359..3853
                     /locus_tag="KW011_002726"
     CDS             3359..3853
                     /locus_tag="KW011_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HBH7661023.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            4107..6398
                     /gene="opgB"
                     /locus_tag="KW011_002727"
     CDS             4107..6398
                     /gene="opgB"
                     /locus_tag="KW011_002727"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HBH7661024.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6537..7559)
                     /gene="lgoD"
                     /locus_tag="KW011_002728"
     CDS             complement(6537..7559)
                     /gene="lgoD"
                     /locus_tag="KW011_002728"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HBH7661025.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7698..8612
                     /locus_tag="KW011_002729"
     CDS             7698..8612
                     /locus_tag="KW011_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH7661026.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8827..10188
                     /locus_tag="KW011_002730"
     CDS             8827..10188
                     /locus_tag="KW011_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH7661027.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10237..11892)
                     /gene="tsr"
                     /locus_tag="KW011_002731"
                     /pseudo
     CDS             complement(10237..11892)
                     /gene="tsr"
                     /locus_tag="KW011_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(12011..12457)
                     /gene="hpaR"
                     /locus_tag="KW011_002732"
     CDS             complement(12011..12457)
                     /gene="hpaR"
                     /locus_tag="KW011_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HBH7661028.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            12729..14018
                     /locus_tag="KW011_002733"
     CDS             12729..14018
                     /locus_tag="KW011_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HBH7661029.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            14015..15481
                     /gene="hpaE"
                     /locus_tag="KW011_002734"
     CDS             14015..15481
                     /gene="hpaE"
                     /locus_tag="KW011_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HBH7661030.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15483..16334
                     /gene="hpaD"
                     /locus_tag="KW011_002735"
     CDS             15483..16334
                     /gene="hpaD"
                     /locus_tag="KW011_002735"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HBH7661031.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16344..16724
                     /locus_tag="KW011_002736"
     CDS             16344..16724
                     /locus_tag="KW011_002736"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HBH7661032.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            16835..17638
                     /gene="hpaH"
                     /locus_tag="KW011_002737"
     CDS             16835..17638
                     /gene="hpaH"
                     /locus_tag="KW011_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HBH7661033.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17649..18437
                     /gene="hpaI"
                     /locus_tag="KW011_002738"
     CDS             17649..18437
                     /gene="hpaI"
                     /locus_tag="KW011_002738"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HBH7661034.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            18612..19988
                     /gene="hpaX"
                     /locus_tag="KW011_002739"
     CDS             18612..19988
                     /gene="hpaX"
                     /locus_tag="KW011_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HBH7661035.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            19998..>20240
                     /locus_tag="KW011_002740"
     CDS             19998..>20240
                     /locus_tag="KW011_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HBH7661036.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT         4871 a         5432 c         5171 g         4766 t
ORIGIN      
        1 cttttaaccc tgcttgcata acaggtattt tactgataac aattccattt ttgcagcatc
       61 ctggcaacat atttcctcca catccactgt ctaaaaatca tgatggttta ttcaggatat
      121 tcattcctta aatgtttcca gcaaattaat agattaagcg aataaattat ccttagcatt
      181 cactttcata tcagcatact cctgctgcac aacaactcca ctgcatgtgt cagaaggtta
      241 gcatcaaatg ataacctggt gaaatagcct ggagacattt aaaatatcta cattaagttg
      301 ttaggaatac ttaatgaaaa acagaaatca cattcattta atttataatt aaatgatatc
      361 ccttcccgca gaatgaaaaa tcacacaacc aatatttatt caatgaaatc tgatggtttt
      421 aacttgttaa cattaaaaaa cgtgcccggt tatctattat cctgattcgt aaaggccaaa
      481 caataaattc attatagtga taatattcat gtgatagttt gtttgcggcg agagataatt
      541 cgctttttat caccgagctt gtcccgataa ccaggattaa tcagcgcaat tttgatctta
      601 acctaccgca aaacaaccgt gcttcatgat acaaatggat tctcattatc tgaacaatac
      661 gcagcacgtc tatgacaaag ggcgagttat gcaaactgag caacagcgag ccgtaacacg
      721 gctttgtatc cagtgtggat tatttctttt gcaacatggt gcggaaagcg cgttggttga
      781 tgagctttcc tcacgactgg gtcgggcgtt ggggatggac agcgtcgaaa gttctatctc
      841 ttcgaacgcc atagtgctga caaccattaa agatgggcaa tgcctgacat cgacacgtaa
      901 aaatcacgat cgcggcatta atatgcatgt ggtgactgaa gtccagcaca ttgtgattct
      961 ggcggagcac catctgctgg attacaaagg cgtagagaaa cgatttagcc aaattcagcc
     1021 attacgttac ccaagatggc tggtagcctt aatggttggc ctttcttgcg cctgtttctg
     1081 caaactcaat aaaggtggct gggatggtgc cgtcatcacc ttctttgcca gtacggccgc
     1141 gatgtatatc cgccagctgc tggcacaacg tcatcttcat ccgcagatca acttttgcct
     1201 caccgctttc gccgccacca ccatttccgg attgcttttg caacttccca ctttcagcaa
     1261 tacacccact atcgcgatgg ccgccagcgt tctgctgctg gtgccgggat ttccgttgat
     1321 taatgccgtc gccgatatgt ttaaaggcca catcaatacc ggactggcac gctgggcgat
     1381 cgccagtctg ctgacactgg ctacctgcgt cggcgtggtg atggcactga cgatttgggg
     1441 gctacgcgga tgggtgtgat cgagtttctg tttgcattgg cgcaggatat gatcctcgcc
     1501 gccattccag cggtcggctt tgcgatggtg ttcaacgttc ccgttcgggc attacgctgg
     1561 tgtgcgctgc ttggcgcgat tggtcatggt tcacgaatga tcttaatgac cagcgggctg
     1621 aatattgagt ggtcaacctt tatggcctcc atgctggtcg gcaccattgg tattcaatgg
     1681 tcgcgctggt atctggcgca tccgaaagtg tttaccgtgg cggccgttat ccctatgttc
     1741 ccgggcatat cggcttatac cgcaatgatt tcggcggtaa aaatcagcca gttaggttac
     1801 agcgaaccgt tgatgattac cctgttaacc aactttctta cagcttcatc gattgttggt
     1861 gcgttatcca tcggtctttc cattcctgga ttatggttgt accgcaagcg ccctcgcgta
     1921 taaaattgcc tcgcttaaag cacacggatg agagacagcc tcctctcctc cgtgtgttac
     1981 tataaaagtt atctcccttc tcgttcatcg ttccatattt gagaaacagt atgtcttcca
     2041 gagttttgac cccggacgtc gttggtattg acgccctggt acacgatcac caaaccgttc
     2101 tggcaaaagc tgaaggcggt gtggttgccg tatttgctaa caatgccccg gcgttttatg
     2161 ccgtcacgcc tgcacgcctg gctgaactgc tggcgctgga agaaaagctg gcgcgtccgg
     2221 gaagcgatgt cgctctggac gatcaactct atcaggaacc gcaagccgct cccgttgctg
     2281 tacccatggg gaaattcgcc atgtatccgg actggcaacc cgatgccgat tttatccgcc
     2341 tggcggcgct atggggtgtg gcactaagag agccggtgac caccgaagaa ctggcctcat
     2401 tcattgccta ctggcaggcg gaaggtaaag tctttcacca tgtgcagtgg caacaaaaac
     2461 tggcgcgcag cctgcaaatc ggtcgtgcca gcaacggcgg actgccgaaa cgagatgtga
     2521 atacggtcag cgaacctgac agtcaaattc caccaggatt cagagggtaa cgatgaaaaa
     2581 cgttggcgac ctgatgcaac gcctgcaaaa aatgatgcct gcccatatca aacctgcatt
     2641 caaaacgggt gaagaactac tggcctggca gaaagaacaa ggagcgatcc gctccgccgc
     2701 tctcgaacgt gaaaatcggg cgatgaaaat gcagcgcacc tttaaccgct ccggtattcg
     2761 gccactgcat cagaactgct cctttgagaa ctatcgcgtt gagtgtgaag ggcagatgaa
     2821 tgcgttaagc aaagcgcgcc agtacgtcga agagtttgac ggcaacatcg ccagcttcat
     2881 cttttctggt aagccaggaa ccggcaaaaa ccatctggcg gcggcaatct gcaacgagct
     2941 gctgctacgc ggtaaatccg tattgatcat caccgtggcc gatattatgt cggcgatgaa
     3001 agataccttc aggaatagcg gtaccagtga agaacaactg cttaacgatc tgagcaacgt
     3061 tgatctgctg gtgatcgatg agatcggcgt gcagaccgaa tcgaaatacg aaaaagtgat
     3121 catcaaccag atcgtcgatc gccgctcttc ttccaaacgc ccaaccggga tgctgaccaa
     3181 cagtaatatg gaagagatga ccaagttgct gggcgaacgc gtgatggacc gtatgcgcct
     3241 gggtaacagt ttgtgggtga tcttcaactg ggatagctac cgtagccggg taacaggtaa
     3301 agagtattaa tccccgtttc tggcggtata ctgcgacaca attccacctt gagtaatgat
     3361 gatgaaaact gttaaacatc ttctgtgctg tgccattgcc gccagcgcat taatttccac
     3421 cggggtgcat gctgcgtcct ggaaagatgc gctctccagc gccgccagcg aacttggcaa
     3481 ccaaaacagc acgacacagg aaggcggttg gtcgctcgcg tcattaacta acttgcttag
     3541 cagcgggaac caggccttaa gcgcagataa catgaacaac gccgcaggca ttctgcaata
     3601 ctgcgcgaag caaaagctgg cgtcggtaac cgatgccgaa aacatcaaga accaggtgct
     3661 ggaaaagctg ggcctgaaca gtgaagagca aaaagaagac accaactatc tggatggcat
     3721 tcagggtttg ctgaaaacca aagatggtca gcaactcaat ctggataaca tcggaacgac
     3781 tccgctggca gaaaaggtga aaaccaaagc ctgcgatctg gtgttaaagc aggggctgaa
     3841 cttcatttcc tgaaccgcga aacgccgcgt tttagctgtt acccggctga cgcggctctc
     3901 tcctgccctt ttccctgctc ctggacggtt ttccccctgt tacacgggaa gcgaccgcga
     3961 tcaaaaaagt ctgtttataa actaattcct aacaacatcg catttttttg acactagaat
     4021 tgcagcaata tggcgacgcc attacattgt gcttctcaaa aagcttaaac gttggctaaa
     4081 cggcccggtt tagtgaggat cgcccgttgt cagaactact ctctttcgcc ctttttctcg
     4141 cctctgtgct gatttacgca tggaaagcgg gacgtaacac ctggtggttt gcagccacat
     4201 taacggtgct ggggctattt gtcgttttaa atatcaccct gtttgccagc gactatttta
     4261 ctggcgatgg tattaacgac gcggttctct ataccttaac caacagcctg accggtgctg
     4321 gcgtcagcaa atacatcctg ccgggtatcg gcattgtgct ggggctgaca gcggtgttcg
     4381 gtgcgctggg ctggatcctg cgccgtcgtc gccatcatcc gcaccatttt ggttacagcc
     4441 tgctggcgct cttactggcg ctgggttcgg tggacgccag cccggcattt cgtcagataa
     4501 cggaactggt gaaatcccag tcacgcgatg gcgacccgga ctttgcggct tattataaag
     4561 agccgtcgaa aactatccct gacccgaaac tcaacctggt ttatatctac ggcgaaagtc
     4621 tcgagcggac ctattttgat aacgaagctt tcccggatct cacgcctgaa ctgggcgcgt
     4681 tgaaaaatga aggcctggat ttcagccaca cgcagcagct gccaggaacg gattacacga
     4741 ttgcgggcat ggtggcttct cagtgcggca taccgctgtt tgcccccttt gaaggcaacg
     4801 cctccgcctc tgtctccagc ttcttcccgc agaacatctg tctgggcgat atcctgaaaa
     4861 actcgggtta tcagaactat ttcgtgcagg gcgcgaatct gcgttttgcc ggtaaagatg
     4921 tgttcctgaa gtcgcatggc ttcgaccact tatacggctc agaagagctg aaaagcgtgg
     4981 tggccgaccc gcactatcgc aacgactggg gattctacga cgataccgtt ctcgatgaag
     5041 cgtggaaaaa gtttgaagag ctttcccgct caggtcagcg attctcactg tttaccctga
     5101 cagtcgatac ccatcacccg gatggtttta tctcgcgtac ctgtaaccgc aaaaaatatg
     5161 attttgacgg taagccgaac cagtcattca gcgcggtaag ttgcagccag gagaatatcg
     5221 cggcgtttat caacaaaatc aaagcgtcac cgtggtttaa agataccgtc atcgtcgtct
     5281 cttctgacca tttagcgatg aacaacacgg cgtggaaata cctcaataag caggatcgca
     5341 ataacctgtt ttttgtcatt cgtggcgaca agccgcagca agagacgctg gcagtgaagc
     5401 gtaacacgat ggataacggc gcgacggtgc tggacattct cggtggcgat aactatctcg
     5461 gacttggtcg tagcagttta tccgggcagt cgatgtcgga aatcttcctc aatatcaaag
     5521 agaaaacatt ggcgtggaag ccggatatca tccgcctgtg gaaattccct aaagagatga
     5581 aagagttcac catcgaccag cagaaaaaca tgattgcctt ctcgggtagc catttccgtt
     5641 tgccgctgct gttgcgggtt tcagacaaac gcgtggaacc gctgccggaa agcgaatact
     5701 cagcaccgct gcgtttccag ctggccgatt tcgctccacg cgacaatttc gtctgggttg
     5761 accgttgcta taagatggca caactctggg ctccggaact ggcactctcc accgactggt
     5821 gtgtctcgca agggcagctt ggcggtcagc aaattgttca gcatgttgac aaaacaacat
     5881 ggcagggcaa aacggcattt aaagatacgg tcatcgacat ggcgcgttat aaaggcaatg
     5941 tcgatacgct gaaaattgtt gataacgata ttcgctacaa agccgacagt ttcatcttca
     6001 acgtcgccgg tgcgccagaa gaggtgaaac agtttagtgg gatttcccgt ccagagtcgt
     6061 ggggccgctg gtccaacgcg cagctgggcg atgaagtaaa aatcgagtac aagcatccgc
     6121 tgccaaagaa atttgacctg gtgattaccg ccaaagcata cggcaataac gccagccgtc
     6181 ctattccggt acgcgtaggc aatgaagaac aaacccttgt gctgggcaat gaagtgacca
     6241 ccaccacact gcatttcgat aacccaaccg atgccgacac actggtaatt gtgccgccgg
     6301 aacctgtctc aaccaacgaa gggaatatcc tcggacactc gccgcgtaag ctcgggatcg
     6361 gcatggtgga aattaaagtg gtggaacgtg aaggataatt cctgactgat gtgtaggtcg
     6421 cataaggtgc gacctacaca ccttctgcgg cctacaaaac atcattgaga aacagaaaaa
     6481 aacagcgccc aacacttccc cggacgctgc atggcaactt taataatctc gccttatcag
     6541 aaagtaatta cacccttaat caactcacga ttgttaatca catcgcgctc gtaggtttct
     6601 gccagcgtgg cgaacggata gcgatgggtt aacatcatgt cagcagtgat ttttccttcc
     6661 gccatcagtc gaccgacttt agcaaaatct tccggcgtgg cgttgcggct acccatcatc
     6721 gtcgtttctt ttttatggaa ttccggatcg gagaactgca actcaccttt aaacaggccg
     6781 acaaatacca ccgtgccgcc gtgacgaatc aaattcacgg tgttattcat cgcatgttga
     6841 ttacctgtcg cgtcgatcac tttctgcgcc agcgaaccac caaactgcgc ccgtagctgc
     6901 gcgtcaaaat cttcggctga cgggtccagt aaaggtaatt ccagacgcgt tgccacatgt
     6961 tcacggcgcg ccggactggt atccgccacc accacctgtg cgccatcggc tttagcgatt
     7021 gccgccgcgc ccagaccgat tggccccgcc ccgaccacca gcacctgctc gccgggagca
     7081 atggctgcgc gacgcaccgc atgagcgcta atagcgaaag gttcaatcaa tgctgccgcc
     7141 tgcgggtcaa taccgtctgc gggcaaaatg ttcgccaccg gcacgctcaa atactcacta
     7201 aaaccgccat cctgatgcac gccaatgact gaaatttttt cacagcaatt ggtacgcccg
     7261 cttttacacg ccgggcattg ctgacaggca acataaggga tcacagcaac ttgctgacca
     7321 tttttaagat cagcaatatt tttacccagc ccaacaatct ccccacatat ttcatggcct
     7381 aaaacacgtg gataactaaa aaatggttga tttccacccc aggcatgaat atcggtaccg
     7441 caaatcccga cagacttaat ttttattaat gcttcattgt cacccggaat aggtatctcg
     7501 cgttgtttcc agactaattc tttcggctgc tggcaaatta aaacattcat cgtagacatg
     7561 attcggctct ccattttttg ttgccttaga tatcaatgaa aatgaaaaat ggcagcggaa
     7621 cggaaaatct gttttgtgaa aacacacgca taaaaatgtt ttaaatcggg ttttaatgga
     7681 ctcacaagga ggaaaatatg agtcgttcac aaaatttacg ccacaatgtg attaaccagg
     7741 tcattgatga tatggcgcgg ggtcatatcc cttccccact tccatcgcaa agtgcgctgg
     7801 cggaaatgta caacatcagc cgcaccacag tgcgtcatat tctcagccac ttacgcgaat
     7861 gcggcgtctt gacgcaggtt ggaaacgact atgttattgc gcgtaaacct gaccatgatg
     7921 acggtttcgc ctgtactacc gcttcaatga gtgaacaaaa caaagtgttt gagcaggctt
     7981 ttttcacgat gataaaccag cgccagttac gcccagggga aacgttttct gaactgcaac
     8041 tggctcgggc cgcgggtgtc agcccggttg tagtaagaga atatctttta aaattcggcc
     8101 gttataatct tattcacagc gaaaagcgcg gccagtggag catgaagcaa ttcgatcaat
     8161 cctacgccga gcagctgttt gagctacgcg aaatgctcga aacacattca cttcagcact
     8221 ttctcaacct gcctgatcat gatccccgct ggctgcaggc gaaaaccatg ctcgaacgtc
     8281 atcggctact gcgtgacaac atcggcaata gctttcgcat gttctcgcaa cttgacagag
     8341 attttcactc cttactgctt tcagctgccg acaatatctt ttttgatcaa tcgcttgaga
     8401 tcatctcagt catctttcat tttcactatc aatgggacga aagcgatctt aaacagcgca
     8461 atatcattgc cgtcgatgag catatgacca ttctcagcgc tctgatttgc cgcagtgacc
     8521 ttgatgccac ccttgcgctc cgtaaccatt tgaattcagc taaacaatca atgattcgat
     8581 ccattaatga gaacacgcgt tatgctcatt aagtacggat taaaaacagc aaactattag
     8641 cgggtgcacg gttttatcaa aagataaata ccgggaaatt cccgcttacc tatgctcaca
     8701 atcagtcgac taaaaatgtt tttgtgagcg gtagtaaagt cctaaaactt taacctgccg
     8761 ttaatgtcaa ccttgcgtaa ttcacaatta cgtaaacaat taaaattata aatacaggag
     8821 tccggcgtgg aaaaagaaaa tatcaccctc gatccgcgtt cttcatttac tccatcttcg
     8881 tcggcagata ttcccgtgcc accagatgga ttagttcaac gcagtacccg aattaaacgc
     8941 attcaaacca ccgccatgtt gttattattt tttgcggcgg taatcaatta tctcgaccgc
     9001 agttcgctgt cggtagcaaa tttaacgatt cgtgaagaat tgggattaag tgccaccgaa
     9061 atcggcgctt tgctctccgt gttttcactc gcttacggga ttgcgcaact tccttgcggc
     9121 ccactattgg atcgtaaagg cccacgcctg atgctgggac tggggatgtt cttctggtca
     9181 ctgttccagg caatgtctgg catggtgcac aactttacgc agttcgtgtt ggtgcgtatc
     9241 ggtatgggga ttggtgaagc gccgatgaac ccatgcggtg taaaagtcat taacgactgg
     9301 ttcaacatca aagagcgcgg acgcccgatg ggcttcttca acgcagcttc taccattggc
     9361 gttgccgtaa gcccaccgat tctggcggcg atgatgctgg tgatgggctg gcgcgggatg
     9421 tttattacca ttggtgtact ggggattttt ctcgccatcg gctggtatat gctctatcgc
     9481 aaccgcgagc acgtagaact gactgccgtt gaacaagctt atctcaatgc aggtagcgtc
     9541 aatgcccgcc gagatccgct cagttttgcc gaatggcgca gcctgttccg taaccgtaca
     9601 atgtggggaa tgatgctcgg attcagtggc atcaactaca ctgcgtggct gtatctggcc
     9661 tggcttcctg gttacctgca aacagcctat aacctggatt taaaaagcac agggttgatg
     9721 gcggctatcc ctttcctgtt tggggctgcc gggatgctgg tcaacggtta cgttactgac
     9781 tggctggtca aagggggaat ggctccgatt aaaagccgta agatctgcat tattgccggg
     9841 atgttctgtt ctgccgcctt tacgctggtc gtaccgcaag cgacaacatc catgacagcg
     9901 gttctgctga ttggcatggc actgttctgt attcactttg ccggaacatc ctgctggggc
     9961 ttgatccacg tcgcagttgc ttctcgcatg actgcgtcgg tgggcagtat ccagaacttt
    10021 gccagcttca tctgcgcctc ttttgcgccg atcattactg gttttattgt tgataccacc
    10081 cactcattcc gtctggcact aatcatctgc ggttgcgtca ccgcagcggg ggcactggcg
    10141 tacatcttcc tggttcgtca gccgatcaac gacccacgta aagattaatc tccttatgcc
    10201 cgataacatt ttgcttatcg ggcattttca tggcgattaa aatgtttccc agttctcctc
    10261 gctatctgcc acggccattt tacgcggcgc agctggcgtc acggttttta ccacagccga
    10321 tgtttcacgc tgctgttgct gaatccggaa cactgccact gcttcggtca ggcgactggc
    10381 ctgctcttcc agcgcggcgg cggcagcggc agactcttcc accagcgcgg cgttctgttg
    10441 agttacccgg tccatctcag caaccgctaa gccaacctga tcgataccac ggctctgctc
    10501 atcagaagca gaagcaattt cgcccataat gtccgtcacg cgggtcacgg cgctgacaat
    10561 ctccgccatt gtttccccgg cgctttcgac cagcgtagag ccaacatcca ctttccccac
    10621 cgagtcttca atcaggcttt taatttcacg agccgcctgg gcgctgcgct gggccagatt
    10681 acgcacttct cccgcgacca ccgcaaaacc gcgcccttgc tcacccgcac gcgcagcctc
    10741 aaccgccgcg ttcaaagcca gaatattggt ctggaaggca atgccgtcaa ttacgctgat
    10801 aatatcggcg attttctgcg aactggtgga gatatcgcgc atagtctgca ccacgttatc
    10861 taccactttg ccgccgcgtt gcgccgtttc agaagcactt aacgccagat ggctggcctg
    10921 gcgcgcattt tcggcgttct gcttcaccgt tgcggtcagt tactccatgc tggctgccgt
    10981 ctcttccagc gaagcggcct gttgctcggt gcgcgaagag agatcgttat tgccggtggc
    11041 gatttcgctg gcaccgctat agatggcatt ggccccgttg cgcacatcac cgacggtacg
    11101 catcagctct ccctgcatat ggcgcaaact ctctgccagt tgccccatct cattagagcc
    11161 atccacctca atcggtttca ccagatcgcc gcctgcaata tgacgaatgc tgtcaatcag
    11221 gcgattcatt ggcgctacca gcgaggcttt aataccgaac cagacggcga agatgaccgc
    11281 cagtacgacg atcatcacgc ccaccagaat ccacatcgcc tggctgtagg aggcattgtt
    11341 atcgctgacg gcgatatcat agagccgatc gttttgctcc atgtaagcca catactgctt
    11401 ctcgaaaccg tcctgatatc cctgggtcgg ctgatcaaag aactcgttga ttttgcctgc
    11461 acctaacagt tggatcagct ccgccagcgc attgtgataa atatcgtaat tacgtttgat
    11521 ctctgccgct gcggcggtgc tctgacgcgg gtcacgcggc aacgcttcgt aatccgccca
    11581 gtttttttcc gcctgtttca gcgaaatact ggcactctgc atcagctcag caacggttga
    11641 accgctacca atattgttct gatccatcat gtagcggata cccgcgcggt tgagggtgtt
    11701 acgcgtctgc aacaacgcga cccagctgcc attcagcgtg gattgctgct ggcgaatggt
    11761 ttgtaaaaca gtgaaatttt ctttgtcatt ctttaaggca ttaaagaaca gaccgcctga
    11821 tgtcagttgt aaaaggccaa aaacggccaa aaccagcagt aagctggtca cgatcttgat
    11881 acgttttaac atggtttctc tttcctgtgg accgatgcaa gattttcggc ctggaaagca
    11941 aaaactttat gaaaaggtgt aacccaccat ccatggaaaa accaggcgct ggttatcaga
    12001 tactaaaaag ttattcatta tcgcccggga tggcttcttg tcgggaattg cccagagcaa
    12061 taaattcctc taacagatgg gttaactgtt gcattttctc ggcagtaaac tgagcttcaa
    12121 tctgccgata ggcttcttca atctgtgtct gagcgcggtt atacagcgcc tgcccctctt
    12181 tggttagcga gatatagagc ttccgctgat cattgatcgg ctttaatcgc aacactaaac
    12241 cgtcgcgttc catccgcgtg aggatcccgg tcaggcttgg gcgcaaaatg caggcgcgat
    12301 acgccagatc gtgaaaatcc attgatgggc tttccgccag aatacgcacg atgcgccact
    12361 gttgctcagt caaattatgc cgtttaacga ttgggcgaaa ataactcatc gccgcttctc
    12421 gcgcctggag caacgcaatg gttagtgagt cgtgcattat ctttcccctg aaaaattcaa
    12481 aatcattaat atagaaacag ttaatcgtca cgttaccggg actgcgctgc ccctgcaatt
    12541 cctgatttta tcaaaattga tgatatttta ttaacttact gatatttaat attttattat
    12601 ctttaaatgt aaatagtttg ttaattagat cacatttaca tcacttactt ttgccaaact
    12661 acgcaatcgg gaataaaagc aatcattaac atattaatga ttaagatgat cgaatccgag
    12721 gagtggtaat gaaaggcact atcttcgccg tagcgttgaa ccatcgcagc caacttgatg
    12781 catggcagga agcgttccag caatccccct acaaagcccc gcctaaaact gcggtctggt
    12841 ttattaaacc gcgcaatacg gtgattggtt gcggtgaacc gattcccttt ccacagggtg
    12901 aaaaggtact gagcggtgcg actgttgcgc tgattgtggg aaaaacggcg acgaaagtac
    12961 gtgaagaaga tgcggcagag tacatcgccg gatatgcgct ggctaacgac gtcagcctgc
    13021 cggaagagag cttttaccgc ccggcaatca aagcaaaatg tcgtgatgga ttctgcccca
    13081 ttggcgaaac cgtggctctc agcaatgtcg ataatctgac catctatacc gagatcaacg
    13141 ggcgtcctgc cgatcactgg aataccgccg atttacaacg taacgccgcg cagttgctga
    13201 gcgccctgag cgaatttgcc acgctgaatc caggcgacgc cattctgctc ggcacgccac
    13261 aggcgcgcgt ggaaatacag ccaggcgatc gcgttcgtgt tctcgcagaa ggtttcccgc
    13321 cgctggaaaa tccggtagtg gacgaacgtg aagtgaccac gcacaagagc ttcccaacgc
    13381 agccacaccc gcacggcacg ctgtttgccc tcggcctgaa ctacgccgac cacgccagcg
    13441 aactggaatt taagccaccg gaagaaccgc tggtgttcct gaaagcgcca aataccctca
    13501 ctggcgataa ccagacctcc gtgcgtccaa acaatattga atacatgcac tatgaagcgg
    13561 agctggtggt agttattggc aaacaggcgc gtaacgtcag cgaagccgat gccatggatt
    13621 atgtcgcggg ctacaccgtg tgtaacgact acgccattcg tgactatctg gaaaactact
    13681 accgccctaa cctgcgggta aaaagccgcg acggactgac gccgatgctt tcaaccatcg
    13741 tgccgaaaga ggcgatcccg gacccgcata atctgaccct tcgcaccttc gtcaacggcg
    13801 agttacgcca gcaaggcacc accgccgatc tgatcttcag cgtgcccttc ctgatcgcct
    13861 acttaagtga atttatgacc ctgaatccgg gcgacatgat cgccaccggc acacccaaag
    13921 gcttatctga cgtggtgcct ggcgatgaag tagtggtgga agtagaaggc gtgggccgcc
    13981 tggtgaaccg aattgtgagt gaggatacag cgaaatgaaa aaagtaaatc attggatcaa
    14041 cggcaaaaat gttgcaggta acgactactt ccagaccacc aatccggcaa cgggtgaagt
    14101 gctggcggat gtggcctctg gcggtgaagc ggagatcaat caggcggtag cggcagcgaa
    14161 agaggcgttc ccgaaatggg ccaatctgcc gatgaaagag cgtgcgcgcc tgatgcgccg
    14221 tctgggcgat ctgatcgacc agaacgtgcc agagatcgcc gcgatggaaa ccgcggacac
    14281 cggcctgccg atccatcaga ccaaaaatgt gttgatccca cgcgcttccc acaactttga
    14341 atttttcgcg gaagtctgcc agcagatgaa cggcaagacc tatccggttg acgacaagat
    14401 gctcaactac acgctggtgc agccggtggg cgtttgtgcg ctggtatcgc cgtggaacgt
    14461 accgtttatg accgccacat ggaaggtcgc gccgtgtctg gcgctgggca ataccgcggt
    14521 actgaaaatg tcggaactct ccccgctgac cgctgacagc ctgggtgagc tggcgctgga
    14581 agccgatatt ccggcaggcg tgctgaacgt ggtacagggc tacggcgcaa ccgcagggga
    14641 tgcgctggtt cgtcatcatg acgtacgtgc cgtgtcgttc accggcggta cggccaccgg
    14701 gcgcaacatc atgaaaaacg ccgggctgaa aaaatactcc atggaactgg gcggtaaatc
    14761 gccggtgctg atttttgaag atgccgatat tgaacgcgcg ctggacgccg ccctgttcac
    14821 catcttctcg atcaacggcg aacgctgcac cgccggttcg cgcatcttta ttcagcaaag
    14881 catctacccg gaattcgtta aacgctttgc cgaacgcgcc aaccgtctgc gcgtgggcga
    14941 tccgaacgat ccgaataccc aggttggcgc gcttattagc cagcagcact gggaaaaagt
    15001 ctccggctat atccgtctcg gcattgaaga aggcgcaacc ctgctggcgg gcggcccgga
    15061 taaaccgtcc gacctgcctg cgcacctgaa aggcggcaac ttcctgcgcc caaccgtgct
    15121 ggcagacgtt gataaccgta tgcgagtcgc ccaggaagag attttcgggc cggtcgcctg
    15181 cctgctgccg tttaaagacg aagccgaagg cttacgtctg gcaaacgacg tggagtacgg
    15241 cctcgcgtcg tacatctgga cacaggatgt cagcaaagtg ttacgcctgg cgcgtggcat
    15301 tgaagctggc atggtgttcg tcaacaccca gaacgtgcgt gacctgcgcc agccatttgg
    15361 cggcgtaaaa gcctccggca ccgggcgtga aggcggtgag tacagcttcg aagtgttcgc
    15421 ggaaatgaag aacgtctgca tttccatggg cgaccatcca attccgaaat ggggagtctg
    15481 atatgggtaa gttagcgtta gccgccaaga tcacgcacgt accgtcgatg tatcactctg
    15541 aactgccagg gaaaaaccac ggttgccgcc agggcgcgat cgacgggcat aaagagatca
    15601 gcaagcgttg ccgggaaatg ggcgtcgata ccattatcgt tttcgatacc cactggctgg
    15661 tcaacagtgc ttatcacatc aactgtgcag accattttga aggcgtctac accagcaacg
    15721 agctgccgca ttttatccgc gatatgacct acaactacga gggcaacccg gagttggggc
    15781 agcttattgc cgatgaagcc ttaaagctcg gcgtgcgggc aaaagcgcac aacattccca
    15841 gcctgaaact ggaatacggc acgctggtgc cgatgcgcta catgaatgaa gacaagcact
    15901 tcaaagtggt ctccatttcg gctttctgca cggttcacga ttttgccgac agccgcaagc
    15961 tgggcgaagc gattctgaaa gcgatcgaac agtacgacgg caccgtggcg gtccttgcca
    16021 gcggttcgtt atcgcaccgc tttattgacg atcagcgtgc ggaagaaggg atgaacagct
    16081 acacccgcga gttcgaccgc cagatggacg agcgcgtggt gaagttgtgg cgcgaaggcc
    16141 agttcaaaga gttctgcaat atgctgccgg agtacgccga ctactgctac ggcgaaggca
    16201 atatgcacga cacggtgatg ctgctgggga tgctcggctg ggataaatac gacggcaagg
    16261 tggagtttat caccgagcta ttcccaagct ctggcaccgg tcaggttaac gctgttttcc
    16321 cgcttcccgc gtaaggacgt tttatgccgc actttatcgt taaatgcagt gacaacatcc
    16381 gcgaagaagc cgacctgccg gggttgttcg ccaaagtgaa tccgacgctg gcagccacgg
    16441 gtatttttcc gctggcgggt attcgcagcc gcgtgcattg ggtcgatacc tggcagatgg
    16501 ccgacgggca gcgtgattat gccttcgtgc atatgacgtt gaaaatcggc gcaggtcgca
    16561 gcctggaaag ccgccagcag gcgggtgaaa tgctgtttga actgattaaa acgcacttcg
    16621 ccgccctgat ggagagccgc ctgctggcgt tgtcgtttga gattgaagag ctgcatccga
    16681 cgctgaattt taaacaaaac aacgtgcacg cattgtttaa gtagcgcgca gattgcccgg
    16741 tggcgctgcg cttaccgggc ctacaaaacc ccaaaccgta caccgtaggc cggataaggc
    16801 gcagccgcat ccggcaatgc cacaggatat cgctatgttc gataaacaca cccacaccct
    16861 gatcgcccag cgtctggatc aggcagaaaa acagcgcgaa cagatccgcg cgatctcgct
    16921 ggattacccg gagatcacca tcgaagacgc ttacgcggtg cagcgtgaat gggttcgcct
    16981 gaaaatcgcc gaaggtcgca cgctgaaagg ccacaaaatc ggcctgacct cgaaagcgat
    17041 gcaggcaagc tcgcagatca gcgaaccgga ttacggcgcg ctgctggatg acatgttctt
    17101 ccacgatggc agcgatatcc cgaccgatcg ctttatcgtg ccgcgcattg aagtggagct
    17161 ggctttcgtg ctggcaaaac cgctgcgtgg gccaaactgc acgctgttcg acgtctacaa
    17221 cgccacggac tacgtgatcc cggcgctgga gctgatcgac gctcgctgcc acaacatcga
    17281 tccggaaacc cagcgcccgc gcaaagtgtt cgacaccatt tctgataacg ccgccaacgc
    17341 cggggtgatc ctcggcggtc gtccgatcaa accagacgaa ctggatctgc gttggatctc
    17401 cgccctgatg tatcgcaatg gcgtgattga agaaaccggc gtcgccgctg gcgtgctgaa
    17461 tcatccggca aacggcgtgg cctggctggc gaacaaactc gcaccgtatg acgtacaact
    17521 ggaagccggg caaatcattc tcggtggttc gttcacccgc ccggttccgg cgcgtaaggg
    17581 cgacaccttc cacgtcgatt acggcaacat gggttccatt agctgccgct ttgtttaagg
    17641 agagaacgat ggaaaacagc tttaaagcgg cgctgaaagc aggccgtccg cagatcggat
    17701 tatggctggg gctgagtagc agctacagcg cagagttact ggccggagca ggattcgact
    17761 ggttgttgat cgacggtgag cacacgccga ataacgtgca aaccgtgctc acccagctac
    17821 aggcgattgc gccctacccc agccagccgg tagtacgtcc gtcgtggaac gatccggtgc
    17881 aaatcaaaca actgctggac gtcggcacac aaaccttgct ggtgccgatg gtacaaaacg
    17941 ctgacgaagc ccgtgaagcg gtacgcgcca cccgttatcc ccccgccggt attcgcggtg
    18001 tgggcagtgc gctggcccgc gcctcgcgct ggaatcgtat tcctgattac ctgcaaaaag
    18061 ccaacgatca aatgtgcgtg ctggtgcaga tcgaaacgcg tgaggcaatg aagaacttac
    18121 cgcagattct ggacgtggaa ggcgtcgacg gcgtgtttat cggcccggcg gatctgagcg
    18181 ccgatatggg ttatgccggt aatccgcagc acccggaagt acaggccgcc attgagcagg
    18241 cgatcgtgca gatccgcgaa gcgggcaaag cgccggggat cctgatcgcc aatgagcaac
    18301 tggcaaaacg ctatctggaa ctgggcgcgc tgtttgtcgc cgtcggcgtt gacaccaccc
    18361 tgctcgcccg cgccgccgaa gcgctggcag cacggtttgg tgcgcaggcc accgccgtga
    18421 agcccggcgt gtattaatgc cggatgcgct gcgcttatcc ggcctacact cgcaccaaac
    18481 cgtaggccgg ataaggcgtt tacgccgcat ccggcaaaaa gccgtaccaa atcgcggacc
    18541 ggataaggcg ttacgccgca tccgccataa accttgtcgt accctacaaa aatcccatta
    18601 gaggaagaaa aatgagcgac acctcacctg ccataccgga gagtatcgat ccggcgaatc
    18661 agcataaagc gctgactgcc ggacaacagg cggttattaa gaagctattt cgccgcctga
    18721 gcgtctttct gttcgtgctg tttatcttct cgttccttga tcgcatcaac atcggctttg
    18781 ccggactcac gatgggacgc gacctcggtc tgagcgccac catgtttggc ctcgctacca
    18841 ccctgttcta tgccgcttat gtcatcttcg gcattcccag caacattatg ctgagtattg
    18901 tcggtgcgcg gcgctggatc gccaccatca tggtgctctg gggcatcgcc tctactgcca
    18961 ccatgtttgc cactggcccc accagcttgt acgtactgcg tatactggtt ggcattaccg
    19021 aagccggctt tctgcctggt attctgctgt atttaacctt ctggtttccg gcctatttcc
    19081 gcgcccgtgc caacgccttg tttatggtag caatgccggt aacgacagcg ttgggctcga
    19141 tcgtttccgg ctacattttg tcgctggatg gcgtcatggc attaaaaggc tggcagtggc
    19201 tgtttttgct ggaaggcttc ccgtcggtat tactcggcgt catggtgtgg ttctggcttg
    19261 atgactcacc ggacaaagct aagtggctga cgaaagaaga caaaaaatgc ctgcaagaga
    19321 tgatggataa cgatcgtctg acgctggttc agccagaggg agccatcagc caccacgcca
    19381 tgcaacaacg cagtatgtgg cgggagatct tcactccggt ggtgatgatg tataccctgg
    19441 cgtatttctg cctgaccaac acacttagtg cgatcagcat ctggacaccg cagatcctgc
    19501 aaagttttaa tcagggcagc agtaatatca ccatcggcat gctggccgcc gtaccgcaga
    19561 tttgtaccat tctcgggatg gtctactgga gccgtcactc agatcgccgc caggaacgaa
    19621 ggcatcacac cgcccttcct tatttgttcg ctgccgcagg ttggttactg gcttcggcaa
    19681 ctgatcacaa catgatccag atgctgggga tcattatggc ttcgaccgga tcattcagcg
    19741 caatggcgat tttctggaca acaccggatc agtccatcag cctgcgggca cgagcgatcg
    19801 gtattgcggt gatcaacgcc actggcaaca ttggttcagc gttaagtccg tttatgatcg
    19861 gctggttgaa agatttgacc ggcagcttta acagtggatt gtggtttgtt gccgcgctgc
    19921 tggtgattgg tgcggggatt atctgggcga ttccaatgca gtcctcccgt ccgcgagcga
    19981 ccctgtaagg aacgacgatg tgtgaccgtc agattgccaa tattgatatc agcaaagagt
    20041 acgatgaaag cctgggcacg gacgatgtgc attatcagtc gttcgcccgc atggcggcat
    20101 tttttggccg ccatatgctg ccacatcgcc acgaacagta ctttcagatg catttcctca
    20161 atagcggaca gattgagcta cagcttgacg atcatcgcta ctcggtggaa gcgcccctgt
    20221 ttgtcctgac gccgccgtca
//
LOCUS       DAESHI010000073        20061 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.074, whole genome shotgun sequence.
ACCESSION   DAESHI010000073 DAESHI010000000
VERSION     DAESHI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20061
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.074"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..496
                     /gene="radC"
                     /locus_tag="KW011_002741"
     CDS             <1..496
                     /gene="radC"
                     /locus_tag="KW011_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBH7661037.1"
                     /translation="LTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            713..949
                     /gene="rpmB"
                     /locus_tag="KW011_002742"
     CDS             713..949
                     /gene="rpmB"
                     /locus_tag="KW011_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBH7661038.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            970..1137
                     /gene="rpmG"
                     /locus_tag="KW011_002743"
     CDS             970..1137
                     /gene="rpmG"
                     /locus_tag="KW011_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBH7661039.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1235..2044
                     /gene="mutM"
                     /locus_tag="KW011_002744"
     CDS             1235..2044
                     /gene="mutM"
                     /locus_tag="KW011_002744"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HBH7661040.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2083..2562)
                     /gene="coaD"
                     /locus_tag="KW011_002745"
     CDS             complement(2083..2562)
                     /gene="coaD"
                     /locus_tag="KW011_002745"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBH7661041.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2570..3847)
                     /gene="waaA"
                     /locus_tag="KW011_002746"
     CDS             complement(2570..3847)
                     /gene="waaA"
                     /locus_tag="KW011_002746"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HBH7661042.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4260..5318
                     /gene="rfaQ"
                     /locus_tag="KW011_002747"
     CDS             4260..5318
                     /gene="rfaQ"
                     /locus_tag="KW011_002747"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HBH7661043.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5315..6439
                     /locus_tag="KW011_002748"
     CDS             5315..6439
                     /locus_tag="KW011_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBH7661044.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6432..7229
                     /gene="rfaP"
                     /locus_tag="KW011_002749"
     CDS             6432..7229
                     /gene="rfaP"
                     /locus_tag="KW011_002749"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HBH7661045.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7245..8261
                     /gene="waaO"
                     /locus_tag="KW011_002750"
                     /gene_synonym="rfaI"
     CDS             7245..8261
                     /gene="waaO"
                     /locus_tag="KW011_002750"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HBH7661046.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFINAKEASPWKNEPFMRPINSNYARYCAKHNFKQNEPINGIMNYIYYFYL
                     KIIK"
     gene            8278..9273
                     /locus_tag="KW011_002751"
     CDS             8278..9273
                     /locus_tag="KW011_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HBH7661047.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNVSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            9283..9975
                     /gene="rfaY"
                     /locus_tag="KW011_002752"
     CDS             9283..9975
                     /gene="rfaY"
                     /locus_tag="KW011_002752"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HBH7661048.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIND
                     TKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            10001..11026
                     /locus_tag="KW011_002753"
     CDS             10001..11026
                     /locus_tag="KW011_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HBH7661049.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            11111..12094
                     /locus_tag="KW011_002754"
     CDS             11111..12094
                     /locus_tag="KW011_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH7661050.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFE
                     LLNQLVESNEKELNDCNLSGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            12140..13393
                     /locus_tag="KW011_002755"
     CDS             12140..13393
                     /locus_tag="KW011_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBH7661051.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLITPKKTITNNPTLLIFISLCLLGIINIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNELRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALLLIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPYIDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQCLLDK"
     gene            complement(13463..14434)
                     /gene="rfaC"
                     /locus_tag="KW011_002756"
     CDS             complement(13463..14434)
                     /gene="rfaC"
                     /locus_tag="KW011_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HBH7661052.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAMI"
     gene            complement(14438..15484)
                     /gene="rfaF"
                     /locus_tag="KW011_002757"
     CDS             complement(14438..15484)
                     /gene="rfaF"
                     /locus_tag="KW011_002757"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HBH7661053.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(15494..16426)
                     /gene="rfaD"
                     /locus_tag="KW011_002758"
     CDS             complement(15494..16426)
                     /gene="rfaD"
                     /locus_tag="KW011_002758"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HBH7661054.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNSQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            16640..17836
                     /gene="kbl"
                     /locus_tag="KW011_002759"
     CDS             16640..17836
                     /gene="kbl"
                     /locus_tag="KW011_002759"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBH7661055.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            17846..18871
                     /gene="tdh"
                     /locus_tag="KW011_002760"
     CDS             17846..18871
                     /gene="tdh"
                     /locus_tag="KW011_002760"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBH7661056.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            19110..20012
                     /locus_tag="KW011_002761"
     CDS             19110..20012
                     /locus_tag="KW011_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBH7661057.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSGMW"
BASE COUNT         5410 a         4372 c         4741 g         5538 t
ORIGIN      
        1 gttaacctct gaatatgaac aatttagtgg cgttcatgga attggcgtag cgaaatttgc
       61 ccagttaaag gggattgctg aactggcgcg gcgttactac aacgtgcgga tgcgtgaaga
      121 aagcccttta ctcagcccgg agatgacgcg ggaattttta caaagccagc tcacgggtga
      181 ggagcgggag atctttatgg tgatctttct cgactcacaa caccgggtta taacgcatag
      241 ccgtcttttt tccggcacgc taaaccatgt tgaagtccat cctcgggaaa ttatccggga
      301 agcgataaaa ataaacgcct cggcgctgat ccttgcacat aatcaccctt cgggttgtgc
      361 tgaacccagt aaagcggata aactcattac tgaacggata ataaagagtt gtcagttcat
      421 ggatttacgc gtgctcgacc atatcgtgat tgggcgtgga gagtatgttt cttttgccga
      481 acgcggctgg atttaacccg ctatgcgcga tccttcggga tctttgtctg ttcgggactt
      541 gagcacatcg ctgagtcagc gtatactacg ccacctttga gaatctcggg tttggcattt
      601 gggcctggca atcgagagtt cacatagaac tgcgatgacc gggctgtaaa gcctgacgag
      661 gcgccaatac cccatacgaa gctcgagcta atttgatttt tggagaatag acatgtcccg
      721 agtctgccaa gttactggca agcgtccggt gaccggtaac aaccgttccc acgcactgaa
      781 cgcgactaaa cgccgtttcc tgccgaacct gcactctcac cgtttctggg ttgagagcga
      841 aaagcgtttt gtcaccctgc gcgtatctgc taaaggtatg cgtgtaatcg ataaaaaagg
      901 catcgataca gttctggctg aactgcgtgc ccgtggcgaa aagtactaag tacttagagg
      961 aaataaatca tggctaaagg tattcgtgag aaaatcaagc tggtttcttc tgctggtact
     1021 ggtcacttct ataccactac gaagaacaaa cgtactaagc cggaaaaact ggaactgaaa
     1081 aaattcgatc cagttgttcg ccagcacgtg atctacaaag aagcgaaaat caaataattc
     1141 tcgctttgat gtaacaaaaa acctcgctcc ggcggggttt tttgttatct gcttgccccc
     1201 atattgattg catctgttca ttcctggaga tgctatgcct gaattacccg aagttgaaac
     1261 cagccgtcgc ggcatagaac cgcatctcgt tggtgcaacc attcttcatg cggtggtgcg
     1321 caacggacgc ttgcgctggc cggtttcaga agagatctac cgtttaagcg accaaccagt
     1381 gcttagcgtg cagcggcggg ctaaatatct gctgctggag ctgcctgagg gctggattat
     1441 cattcatttg gggatgtctg gcagcctgcg catccttcca gaagaacttc cccctgaaaa
     1501 gcatgaccat gtggatttgg tgatgagcaa cggcaaagtg ctgcgctaca ccgatccgcg
     1561 ccgctttggt gcctggctgt ggaccaaaga gctggaaggg cacaacgtgc tggctcatct
     1621 tggaccggag ccgcttagcg acgatttcaa tggtgagtat ctgcatcaga agtgcgcgaa
     1681 gaaaaaaacg gcgattaaac cgtggctgat ggataacaag ctggtggtag gggtagggaa
     1741 tatctatgcc agcgaatcac tgtttgcggc ggggatccat ccggatcggc tggcgtcatc
     1801 actgtcgctg gcagagtgtg aattgttagc tcgggtgatt aaagcggtgt tgctgcgttc
     1861 gattgagcag ggtggtacaa cgctgaaaga ttttctgcaa agtgatggta aaccgggcta
     1921 tttcgcgcag gaattgcagg tttacgggcg aaaaggtgag ccgtgtcggg tgtgcggtac
     1981 gccgattgtg gcgactaaac atgcgcagcg ggcaacgttt tattgtcggc agtgccagaa
     2041 gtaattcata cgcgccggat ggcataccat ccggcataaa cgctacgcta acttcgccac
     2101 cagcgcctga tggacattct ccggcaggaa atgggtgaca tcgccctgat ggcgcgccac
     2161 ctctttcacc aacgatgaag agataaacga ccactctttc gacggcatca gaaacacact
     2221 ttccagttcc ggcattaagt ggcgattcat atgcgccagc tgcatttcat attcaaaatc
     2281 cgccaccgca cgcaggccac gaatcagcac cgtagcgtgt tgattacggg cgaagttcgc
     2341 catcaaatca ctaaacccaa ccacttccac gttccccaga tgcgcggttg cctgctgtgt
     2401 cagcgccaca cgctcttcca gggtaaacat cggtttttta ctggggctgg cggcaatcgc
     2461 cagaataacg tgatcgaaca tttgcgtggc gcgcgtcacg atatcgatat gaccattggt
     2521 aatgggatcg aaagtacccg gataaatcgc ccgtttttgc ataacaacct caatgcgttt
     2581 tcggtggcag gtaaggttcc agcagttgaa gcagacgctg tagcgcgccc tggttttgat
     2641 acagtacttc aacggcatga cggccataga aactacggta atcggcgtcg gtgagtaaag
     2701 aggaaacctc ttttgcaagc gtagtggcat cggtaacggt aatcagcccg cttgcctgct
     2761 ccagccgcgc gcaaatgtct ttgaaattaa aagtatgcgg ccccatcaat accggaatag
     2821 cgtgtgcggc agcttccaga ggattatgcc ctccacgttc aaccagtgaa ccgccaacaa
     2881 aggcgagatc ggcaatgccg tacagtaaca tcaactcgcc catcgtatcg ccaaccacaa
     2941 cctgcgtgct ggtggagggg acttcccctg aagagcgtgt gatatagctt agtccagcct
     3001 ggcggacaag gttaatcgca tccgggaagc gttctggatg acggggtacc aggatgagca
     3061 ataaattcgg gaattgctgt aataatgcct gatgtgcggc gatcaccaca ctctcttcac
     3121 cttcgtgagt gctggtggca atccataccg ggcggtgtgg tgcccactgg cggcgcagcg
     3181 tcacggcttt agcagccaac tgcggcgtta cagaaatatc gaatttcagg ctaccggtaa
     3241 cggttacctg gttatttttt gcgcccagcg ccacaaaacg tgcaccatct tcttcatttt
     3301 gcgcagcaat cagcgtaata cgacgcagta agcggcggac gaatttaccc agtttggcat
     3361 aacctgcggc cgagcgggca gagagtcgcg cgttagcgat caccagcgga attttacgtt
     3421 tatgtagcgc cgcaatcagg ttaggccata gttcggtttc cataatcaac accagtttgg
     3481 ggtcgacttt attcaggaaa cggttgagcg catcgggcag gtcatacggc agataaacgt
     3541 gctgaacatc cttcccgaaa gccgattgta cgcgctccga accggttggc gtcatggttg
     3601 taacggtaat cggtaaatca ggataacgat ggcgcagcgc gcgcaccaac gggatcgccg
     3661 ccagagtttc accgacggag acggagtgca gcataatgcc gcctggtttt agcggatggc
     3721 ggtaaaaacc gtaacgttca ccccagcgtt ttcgataggc cggagcctta cgtccgcgca
     3781 cccagagccg tatccagatc agcggctgaa taaggtagag aagggcggtg taaagcaatt
     3841 cgagcatagt aaatagctga cttatggatg tgctggggat tctatgtatt tagctgtggc
     3901 tttaccatta cttttcccgt ttttgactta aatagcaatt tacccgccaa ttcttgctga
     3961 acatctatac tttttttgta tttttatgcg attcattgat ttctgctgta cgtgcaggaa
     4021 ggaatcgcag agcaacagtg aaagaatatg gttgctaatg attttatttg attgatttct
     4081 ttattgaaaa tcaacaatgt gcttaaaaag cattcgggtc ggattgccgc gaaagaagtg
     4141 atacactagc gcgtcaatat cagtgcggta tcagtattca ggtagctgtt gagcctgggg
     4201 cggtagcgtg ctttttctgc ttcaccttaa tcggataatc tcaacaaaaa gagttacttg
     4261 tggataagcc atttcgaaga attttgctca ttaagatgcg ttttcatggg gatatgttat
     4321 taactactcc cgtcattagt tcgctgaaaa aaaattaccc tgacgcaaaa atcgatgtgc
     4381 tgctttatca ggacaccacc ccgatcctgt ctgaaaatcc agagattaac gcgctctacg
     4441 gcataaaaaa taaaaaagca aaagcctcag aaaaaattgc caactttttt catctcatca
     4501 aggtattacg tgccaataag tatgacctta tcgtcaatct taccgatcaa tggatggttg
     4561 ctatactggt tcgcttatta aatgcccgtg tgaaaatttc ccaggattat catcatcggc
     4621 agtctgcttt ttggcgtaaa agtttcaccc atttagtgcc gttgcagggt ggaaatgtgg
     4681 tggaaagtaa cttatccgtg ctgacaccat tgggacttga ttcgttggtg aagcagacaa
     4741 ccatgagtta cccgcctgca agctggaaac gtatgcgtcg cgaacttgat cacgctggtg
     4801 ttggacaaaa ttatgtggtt atccaaccta cggcgcggca aatcttcaaa tgctgggaca
     4861 acgccaagtt ttccgctgtg attgatgcct tacatgctcg tggttatgaa gtcgttctga
     4921 cgtccggccc agataaagac gatctggcct gcgtcaatga aattgcgcag ggatgccaga
     4981 cgccaccagt aacggcgctg gctggaaagg tgaccttccc ggaacttggt gcgttaatcg
     5041 atcatgcgca gctgtttatt ggcgttgatt ccgcaccggc gcatattgcc gctgcagtta
     5101 atacgccgct gatatcgctg tttggtgcga cagaccatat tttctggcgt ccttggtcaa
     5161 ataacatgat tcaattctgg gcgggagatt accgggaaat gccaacgcgc gatcagcgtg
     5221 accgaaatga gatgtatctt tcggttattc cggcggcaga tgtcattgct gctgtcgata
     5281 aattactgcc ctcctccacg acaggtacgt cgttatgatc gttgcttttt gtttatataa
     5341 atattttccc tttggcggtt tgcagcgcga ttttatgcgt attgctcaga cagtcgccgc
     5401 ccgaggtcat catgttcggg tttataccca gtcgtgggaa ggcgaatgcc ctgatgtatt
     5461 tgaactgatc aaagtgccgg ttaaatcgca taccaatcac gggcgcaatg cggagtattt
     5521 tgcctgggtg caaaaacatt tacgcgaaca tcccgtcgat aaagtcgttg gattcaacaa
     5581 aatgccgggg ctggacgttt attatgccgc tgatgtttgt tatgccgaga aagtcgcgca
     5641 ggaaaaaggc tttttctatc acctgacgtc acgttatcgc cattatgccg cctttgagcg
     5701 ggcaaccttc gaacagggca agccgacaca gctgctgatg ctgacagata agcaaatcgc
     5761 cgatttccag aaacattatc agactgaagc ggagcgtttt catattctgc caccggggat
     5821 ttatcctgat cgtaaatata gccagcagcc agctaatagc cgtgaaatct tccgtaagaa
     5881 gaatggaata accgaacaac aatatttatt gttgcaggtc ggttcagact tcacgcgtaa
     5941 aggtgtcgat cgttccattg aagcacttgc ttcgttaccg gattcgctgc gccacaacac
     6001 attgctatat gttgttggac aggataagcc gcgaaaattt gaggcgctgg cagaaaaacg
     6061 cggcgtgcgc agtaatgttc acttcttctc ggggcgcaac gatgtctcgg aattaatggc
     6121 ggcggcggat ttattactgc atcctgccta ccaggaagca gcgggaattg tgctactgga
     6181 agcgattact gcaggattac cggtactaac aacagccgtt tgtggctatg cgcattatat
     6241 tgtcgacgcc aattgcggcg aggctattgc tgagccattc cgccaggaaa cattgaatga
     6301 gattttacgc aaagcgttaa cgcaatcttc attgcgccag gcctgggcgg aaaatgcgcg
     6361 acattatgct gatacacaag atttatacag tctgccagag aaagcggcgg atatcataac
     6421 gggtggtctg gatggttgaa cttaaagagc cgtttgccac gttatggcgc ggtaaagatc
     6481 cttttgagga agttaaaacc ttgcagggtg aggtttttcg tgaactggaa actcgccgca
     6541 ctctgcgatt tgaaatggcg ggcaaaagct attttctcaa atggcatcgc ggcacgaccc
     6601 tgaaagagat aatcaaaaat ttactctcat tgcggatgcc agtattaggc gcagaccgcg
     6661 aatggaatgc gattcatcga ctgcgggatg tcggcgttga tactatgtat ggggtggcat
     6721 ttggcgaaaa aggtattaat ccgctgagca gaacctcgtt tattattacc gaagatctga
     6781 ccccaaccat cagtctggaa gattactgtg ctgactgggc gaccaaccca ccagatgttc
     6841 gcgtaaagcg tatgcttatt aagcgtgtcg cgacgatggt gcgcgatatg catgctgtgg
     6901 gcattaacca ccgtgactgt tatatctgtc atttcctgct gcacttgcct ttttccggta
     6961 aggaagagga gttaaaaatt tcggtaattg acctgcaccg ggcgcagctt cgcacgcgcg
     7021 ttccacgtcg ttggcgcgat aaagatctta ttgggcttta tttttcttcg atgaatatcg
     7081 gcctgactca gcgggatatc tggcggttta tgaaagtgta ttttgtcgcc ccgcttaaag
     7141 acattctcaa gcaggaacaa ggactgctgt cgcaagcaga agcaaaagcc acaaaaatca
     7201 gggaaagaac gattcgaaaa tcgttgtaat ttccggagag aaatatgagt gcccactatt
     7261 ttaatccaca agagatgatc aataagacaa tcatcttcga tgaaaggcca gcggcgtcag
     7321 tggcatcatc attccatgtt gcttatggca ttgataaaaa ctttcttttt ggttgtggtg
     7381 tttcaatcac gtcagttttg ttacataaca acgacgtgag ttttgttttc cacgttttta
     7441 ttgatgatat ccctgaagcc gatatccagc gtttagccca attggcgaaa agctatcgta
     7501 cctgtatcca gatccatctg gtaaattgtg aacggcttaa ggcattaccg acgaccaaaa
     7561 attggtctat tgccatgtat ttccgttttg taattgcaga ttactttatt gatcaacaag
     7621 ataagatcct ttacctggat gctgatatcg cctgtcaggg aaacttaaag ccgctgataa
     7681 caatggatct tgccaataac gttgctgctg ttgttactga acgcgatgct aactggtggt
     7741 cgttacgggg tcaaagtctg cagtgtaatg aacttgaaaa gggttacttt aattcaggtg
     7801 tcctgttaat taatacacta gcgtgggcgc aggagtccgt ttctgctaaa gcgatgtcga
     7861 tgcttgctga taaagccgtc gtttcccgtt taacctatat ggatcaagat atacttaatc
     7921 ttatcctgtc agggaaagtt aaattcattg atgctaaata caatactcaa tttagtttaa
     7981 attatgaatt aaaaaaatca tttgtttgtc caattaatga tgaaaccgta ttaattcatt
     8041 atgtcggccc gacaaaaccc tggcattact gggccggtta tccaagtgcg caacctttta
     8101 tcaacgccaa agaagcatcg ccctggaaaa atgaaccgtt tatgcggcca attaattcaa
     8161 actatgctcg ttattgcgcc aagcataatt ttaaacaaaa tgaaccaatt aacgggataa
     8221 tgaattatat ttattatttt tatttaaaga taataaaatg atattgagaa aagttaaatg
     8281 aatgaattta taaaagaacg gttttcgtat ttagcagata ataaaaaaga aaacgcccca
     8341 gaactaaatg tttcctacgg tatcgataag aattttttgt atggtgctgg cgtttcaatt
     8401 tcttccgttt tgattaataa ttcagatatt aattttgtct ttcatgtttt cactgattat
     8461 gtggatgatg attatttaaa gtcatttaat gaaacagcaa aacaatttaa tacctcaatt
     8521 attgtatatt taattgaccc aaaatacttt gctgatctgc cgacgtcaca gttttggtcg
     8581 tacgcgacat acttcagggt attgtccttt gaatatctga gtgaaagtat ttccacactg
     8641 ctgtatctgg atgccgatgt cgtttgtaaa ggaagtctga agcctctcac agaaattata
     8701 tttaaagatg agtttgctgc ggtcattcct gacaatgtta gtactcaggc ggcatgtgca
     8761 aaacgcctca acattcccga gatgaatgga cgttatttca atgcaggcgt tatctatgtc
     8821 aatcttaaaa aatggcatga agcaaatttg acaccgtatt tactcacgct tttacgaggg
     8881 gaaactaaat atggctctct taaatattta gatcaggatg cgttgaatat cgcatttaat
     8941 atgaataata tctacctcgc gaaggatttt gatactattt ataccctgaa aaacgaactt
     9001 catgatcgta gtcatcgaaa gtatcagcaa accattaccg ataaaacagt attgattcac
     9061 tatacaggga taactaaacc atggcatagc tgggctggtt atccgtctgc atcatacttt
     9121 aatatcgcgc gtgaacaatc tccctggaag aaatatcctc ttaaagaggc gcggactgtt
     9181 gcagaaatgc agaaacaata taagcatctg tttgcccatg gtgagtatat taaaggcata
     9241 acttcattaa ttaagtacaa gcttaagaaa taaattactc ttatgattac aagtatacgc
     9301 tatcgcggct tctcatttta ttacaaagat aatgataata aatataaaga aatctttgat
     9361 gaaatactgg cctataattt taaaacagta aaagtattac gtaatattaa tgataccaaa
     9421 gtatcattga ttgatacgaa atatggtcgc tatgttttta aagtctttgc cccgaaaacg
     9481 aaaaggaatg aacgtttttt aaaatctttt gtaaaaggtg actactatca gaatttgatc
     9541 gttgaaaccg atcgtgtcag aagtgcaggt cttacttttc ctaatgattt ttattttctg
     9601 gctgagcgta aaattttcaa ctatgccagt gtttttatca tgcttatcga gtatgtcgag
     9661 ggcgtagagc tcaatgatat gccgatcatt ccagaagatg ttaaagcaga aattaaggct
     9721 tcaatggaaa aattgcatgc cttaaatatg ctatcgggcg acccacaccg gggtaacttt
     9781 atcgttagta aagatggtgt caggataatt gacctatccg gtaaaagctg tacggcagag
     9841 cgtaaagctc gtgatcgtct ggccatggaa cgtcacctgg gtattgccaa cgaaataaaa
     9901 gattatggct actattcagt aatctacaga accaaactac gcaaatttat taaaaaatta
     9961 aaaggcaaag cgtaaaccac acagtcaaaa cggaaccaac atggatttat tagctgagag
    10021 tattactgaa gtcgctgtct ctggggaaat tgctaacacc gatcgtgtgt taaatatcgc
    10081 ttacggtatt gaccgtaact ttttatttgg tgcggcagta tctatgcaat cagttgttat
    10141 gcataatccg gaccttgcgg ttaagtttca tctctttacc gactacattg atgaagatta
    10201 tctacaacgt gttaatgctt ttaccagcaa aaatgctaac gttgaagtaa gaatttataa
    10261 agtctccagt gcctttattg atatcttccc cagcctgaaa cagtggtctt atgcaacatt
    10321 cttccgttta gttgcgttcc agtatctgag tgaaactatt gaaaatctgt tatatatcga
    10381 tgctgatgtc atctgtaaag gctcattagc tggattgctt gatattaatt ttgatgggga
    10441 taagtttgca gctgttatta aagatgtgcc ttttatgcag gaaaaaccag cgaagcgtct
    10501 ggctatagag ggacttccag ggaattattt caacgccggt gtagtatatc tgcagcttga
    10561 agcatgggcg aaaaatgatt ttatgaataa agccattgct atgctggcaa gtgacccgca
    10621 gcacacgaag tataaatgcc ttgatcagga tattttaaat attctgttct ttggtcattg
    10681 tatttttatt agcggcgatt atgattgctt ttatggcatc gattatgagt taaaaaataa
    10741 aagcgatgaa gattataaaa agaccattac cgatgatact aagctgattc attatgttgg
    10801 cgtaacgaag ccctggaacg actggacgaa ttatccctgc cagaagtatt ttaatgaggc
    10861 ttatcaggct tcttgctgga atgatgtggc gtttattcca gccacgaatg aaaagcagta
    10921 tcaagtgaaa tatcaacatg caaagaaaaa tggtgatacg tttaacgctt ttatttactt
    10981 tattaaattt aaattaaata agtataaaag aaaactattt ggataataat aaagatcatg
    11041 gatgtctgag ttacaataat aattaaggat gagtactatc atattttatt agttcaggca
    11101 agagaacacg atgagcaatg attacccttt agtatccata ataataccga cgtataattc
    11161 atctgattac attactgaaa ctctaacgaa attagaaaaa caaacttacc caaattttga
    11221 aattgttatt gttaatgatg gttctaaaga taacacatca aacgttttga gagagtatgg
    11281 gttaacccac tctcgattaa ttattatcaa taaagaaaat ggcggtgttt cgtctgccag
    11341 gaatacaggt atccgcaagg cgcaaggaca gtttatatgt tttatggatg atgatgatga
    11401 gatagatcct aactatctgc tgaagatgta taccagacaa catgagacgg gaggggatgc
    11461 gatttattgt gggctttatg gccattatat aaaagatggt gttacttact cacctataaa
    11521 tacagagttt aatgaaggat ctttactttt cgactttttt tataaaaagg ttagattcca
    11581 tatagggtgc ttgtttataa gaaaacaact tctggaagag aataatcttt tttttgatga
    11641 agatttacga ctaggagaag atctggattt tatctatcga ctgctaatta catgcgatat
    11701 gtatgcggtt ccatattata tgtataagca taactataga gaaaattcct taatgaactc
    11761 atgtagaacc atcactcatt atcgacatga gtcatttgcg cacgaaagaa tctactcttc
    11821 tgtgatgcag ttatacaaag gtaacaggaa agaagaaatt catacattat tgagtcaaaa
    11881 tagagcttat cataaaactc gttatttatg gaatgttcta cttaatggtg attttgaact
    11941 attaaatcaa ttagttgaaa gcaatgaaaa agaattgaat gattgtaatc tttctggcaa
    12001 gagagataaa agacgagcaa aaatattagc atcaaaaaat tatattctct ggaggatggt
    12061 aagactggta aataggaaaa agaataaacg ttagatcttg cattaaataa tccgtcctcg
    12121 tagcataggt tgaaactata tgtcattttg ttggaatgaa attaattctg gtataaagtc
    12181 tttaattctc atattatgta ttttttcttt gatgactttg tctttatggg atgatgttgc
    12241 aacaaagttt cttcatgcag ctggaattat atctgcattg tattttctaa taacaccaaa
    12301 aaaaacaata actaacaatc ctactttgtt aattttcatc tcattatgtc ttttgggaat
    12361 cataaatatc atctggtatt cacattataa agtttcaggc tctgtttata ccaatgcata
    12421 tcgtggccca atggaaactg ggaaaattgc cttgtgtagt gcttttattt tcttggttct
    12481 ttttgctaaa aatgagttga gaacaaaaat aaaatttggg aaactaattc tgttcgcatc
    12541 cctggcaacg cagttacttt tttttgcgca tgccatgtgg caacatttct atttaaacgt
    12601 tgaccgtgtt gcattatcag cttcccacgc tacaacagca ggctacatca tccttttccc
    12661 ttctttactg gcatcaattc tcattttaaa atccgacttt agacataaaa caacattata
    12721 tacaattaac ttcatgctta gcttatgtgc tgtcatagta actgaaacgc gtgcagccat
    12781 attagtgttt ccattctttg cgttactatt aatcgtaatg gatagttata ttaataagcg
    12841 aattaattat aagttatatt gttttattgc gattgcatta ttagcaggtg tattttcttt
    12901 taaagataca ttgcttatga gaatgaatga cttaaataac gatttagtta attattcgca
    12961 tgataacacc agaacttcag tcggtgcccg tctggcaatg tatgaagttg gcttaaaaac
    13021 atattctcca ataggacaat cactggaaaa acgagcggaa aaaatacatg aactagaaga
    13081 aaaagagcct agattgagtg gcgctttacc ctatatagat tctcatttgc ataacgatct
    13141 catagatacg ttatcaacgc gtggtattcc tggagttgta ttaacaattt tagccttttc
    13201 agcaatactc atatatgcct taagaactgc taaagaacct tatattttaa tcttgctttt
    13261 ttcactactg gtagtaggcc taagtgatgt aatactcttt tctaaaccgg ttccgactgc
    13321 tgtgtttgtc accataatat tgctttgtgc ttattttaaa gcacaatcag accaatgttt
    13381 attagataaa taatgaatat gtgaaataaa atcagcaagg aggctgattt tatttcaaaa
    13441 ttatctatta aggggttgta atttaaatca tagcagcgtt ttcttcaatg aatcgcttaa
    13501 cagcatttgc tgtcaattga gacaactcat tccccggagc cctacaaacc atctgattct
    13561 tcccatatcc accaattaat cccggatcgg ttggtccata aaccgtgata ttgggtctat
    13621 ccagtgccgc cgttaaatgg cttaaccccg tatccaccga cactacaaat ttagccccgg
    13681 ccagcacgcg ggcaacgcct tccagactca tcttcggcaa tacttcaaca taagcaaatc
    13741 cttccgccag tcgtttcgcc cgctcttcct catgcggcgc gccccacgga agtttaatcc
    13801 gtattcctga atcagccagt aaaccaatca attctcgcca gtgttcttcc ggccagtgtt
    13861 tatcatcacg ggtcgtcgca tgaagaaata cggcatattc gccagcatct gtaggcaggt
    13921 tcgtcaggaa atgctgtgca atagcataat cgccctgggt ttgcggtttg ctatagccca
    13981 gacttttggc aaacagttcg cgggtgcgtt ctacggcgtg ctgctgtttt gcaatatgat
    14041 gcttacgatt gtaaaacaga ctggctaaag gttcgcgagc ggtttgccag tccatgccat
    14101 gctttacgcc atgcgccaga cgcgtcacca gcgccgcgct ttttaccagc ccctgagcgt
    14161 cgataactgc gtcatagttc tctgcttgta gcgcttcacg aaacgctttg cgttccgctt
    14221 ttatgggggc cgagaaccag gctttacgcc agcgacgtat tgccacagga ataactcgct
    14281 caacggcggc gtgccaggaa ggaatctgtg cgaacccttc ttccaccacc cagtcaaact
    14341 taatccctgg gattgcctgc tgggcatcag tgagtgcggg caacgtatgg agaacatcgc
    14401 ccatcgacga tgttttaacg atcaaaaccc gcatccgtca ggcttcctct tgtaacaata
    14461 gcgcgttgag ttcttccagt acgcgctggg gagtaatgtc gattaagctc tggtgataac
    14521 cctccgcagc gtcaccttta cgcactttgt gatagccggt aatcaggcgg atcacgcgcg
    14581 ctttatggga tagcggcggt gtgaagtccg ggctactcgg accatacagg gcaaccagcg
    14641 gacgattgag cgccgccgca acgtgcatca ggccagaatc gttagtgaca atggctttac
    14701 aggctgcaat caggataacc gcttgatcaa gctgtgtttc ccccgccagg ttccgacacc
    14761 atgcctgctg ctcggtattc aaagcggcaa gaatctcatt gcccgcttca tgatctttcg
    14821 ccgagccaaa cagaaccacc tgataacctt catcaatcag ctgctttgcc agttccgcat
    14881 agtggtagtg tggccagcgt tttgccggac caaactccgc acccgggcaa aaaccaatca
    14941 tcggacgttc tgatgaaagc gaaaattgat tacaggtata tgatttttca ccttcgctca
    15001 cctgcaattg cggccataac aacggttttg gtagatcttg tgcggtacgc atcacgcctt
    15061 tgtcataggc cagcgcgaca tagcgttcca ccattagcgg ccaggcttct ttatcgagca
    15121 cgcgtaaatc gttgagtaaa ccgtagcgca tctcgccgcg ccagccggtg cgatgaggaa
    15181 tacccgcgaa gaaaggcact aatgcagatt tgaaggagtt gggtaagacg taggcgcggt
    15241 cgtagcgctt ttcacgcagg ctatgaccca gtttgcggcg ttcgccgatt tccagcgctc
    15301 cgtgaccgag aggcatagga atagcttcgt taacttccgg catccgcgat aataatggac
    15361 ggcaccatgc cggtgccatc acatcgatta tcgcctgggg atagcgcgcc tggagcgtgc
    15421 gatagagact ttgcgacatc atcatgtcgc caacccaaga cgggccgatc accagtattt
    15481 tcatgcagag ctcttatgcg tcgcgattca gccaggccat gtattccgtt acaccttcag
    15541 caacggtttt gaacggtttg tcgtaacccg ccgcgcgcag atttgtcaga tctgcctgag
    15601 tgaacgcctg gtagcggcct ttcagcttat ccgggaacgg aatgtattcg atctggcctt
    15661 tcttgtgata agccagcgta gcatcagcta cagcctggaa ggattccgca cgaccagtac
    15721 cgaggttgaa gatgccggaa acgccatttt ccaggaacca cagattcaca tcagccacgt
    15781 cgcccacata gacgaagtcg cgtttgaagt tctcgctacc ttcaaacagc ttcggtgatt
    15841 caccgttgtt aagctgggag ttgagatgga aagcgacgct cgccatgctg cctttatggc
    15901 cttcacgcgg tccataaacg ttgaaatagc ggaagccaac aatctgcgag ttagcttcag
    15961 gcaggatttg gcgaacatat tcatcaaaca ggaattttga gtaaccgtag acgttcaacg
    16021 gtttttcgta ctcgcgggat tcaataaagt cggaggtgcg tccgccgtag gtggctgcgg
    16081 aagaggcata caggaacggg atttcgcgct ccagacagta gtgcagcagc tctttggagt
    16141 attgatagtt gttatccatc atatacttgc catcccactc ggtggtggaa gagcacgcgc
    16201 cttcgtggaa aatcgcttcg acatcgccga actcttcgcc agccataatc tggatcagga
    16261 agtcttcctt atccatatag tccgcgatat ccagatccac caggttcaca aacttggtgc
    16321 cgtctttcag gttgtccacc accagaatat cggtgatgcc tttatcattc agggccttaa
    16381 cgatgttgct gccgataaag cccgcgccgc cggtaacgat gatcataact gtaaccttcg
    16441 aattatggag tcagagacaa tctcagacac gaatacttct atcatatcac tacacggcgt
    16501 tggcttcagc cattcgccaa aagtgagatt gatggacgtt atttatcctg aattttgcag
    16561 aagtgttaac gcgtcatctc gtcgcgacct ataagtttgg gtaatatgtg ctggaatttg
    16621 ccctgtctgg agaatcgcaa tgcgtggaga attttatcag cagttaacca acgatctgga
    16681 aaccgcacgg gcggaagggt tgtttaaaga agagcgcatt attacgtctg cgcagcaagc
    16741 agatatcact gtggctgatg gaagccacgt cattaacttt tgtgccaata actatctcgg
    16801 gctggcgaat tatccggatc tgattgcggc ggcaaaggcg ggaatggatt ctcacggttt
    16861 cggcatggct tcggtgcgtt ttatttgcgg cactcaggac agccataaag agcttgaaca
    16921 aaaactggcg gcattcctgg ggatggaaga tgcgattctc tactcttcct gctttgatgc
    16981 taacggtggc ctgtttgaaa cgctgctggg cgcggaagac gccattatct ccgacgcact
    17041 gaaccacgcg tctattattg atggtgtgcg tctgtgtaaa gctaaacgct atcgctatgc
    17101 caacaacgat atgcaggagc tggaagcacg tctgaaagaa gcgcgtgaag ccggtgcgcg
    17161 tcatgtgctg attgccaccg atggtgtgtt ctcgatggac ggcgtgattg ccaacctgaa
    17221 gggcgtttgc gatctcgcag ataaatatga tgccctggtg atggtagacg actcccacgc
    17281 ggtcggtttt gtcggtgaaa atggtcgtgg ttcccatgaa tactgcgatg tgatgggccg
    17341 ggtcgatatt atcaccggca cgctcggtaa agcgctgggc ggggcttctg gtggttatac
    17401 cgcggcgcgc aaagaagtgg ttgagtggct gcgccagcgt tctcgtccgt acctgttttc
    17461 caactcgctg gcaccggcca ttgttgccgc atccatcaaa gtactggaga tggtcgaagc
    17521 gggtagcgag ctgcgtgacc gtctgtgggc gaacgcgcgt cagttccgtg agcaaatgtc
    17581 ggcggcgggc tttaccctgg cgggagccga tcacgccatt attccggtca tgcttggtga
    17641 tgcggtagtg gcgcagaaat ttgcccgtga gctgcaaaaa gagggcattt acgtcaccgg
    17701 tttcttctat ccggtcgttc cgaaaggtca ggcgcgtatt cgtacccaga tgtctgcggc
    17761 gcatacccct gagcaaatta cgcgtgcagt agaagcattc acgcgtattg gtaaacaact
    17821 gggcgttatt gcctgaggat gtgagatgaa agcgttatcc aaactgaaag cggaagaggg
    17881 catctggatg accgacgttc ctgtaccgga actcgggcat aacgatctgc tgattaaaat
    17941 ccgtaaaaca gccatctgcg ggactgacgt tcacatctat aactgggatg agtggtcgca
    18001 aaaaaccatc ccggtgccga tggtcgtggg ccatgaatat gtcggtgaag tggtaggtat
    18061 tggtcaggaa gtgaagggct tcaagattgg cgatcgcgtt tctggcgaag gccatatcac
    18121 ctgcggtcac tgtcgcaact gtcgtggcgg tcgtacccat ctgtgccgca acacgatcgg
    18181 cgtcggcgtt aaccgcccgg gctgcttcgc cgaatatctg gtgattccgg cgttcaacgc
    18241 cttcaaaatt cccgacaata tctccgacga cctggcttcc atttttgatc ccttcggtaa
    18301 cgccgtgcat accgcgctgt cgttcgatct ggttggcgag gatgtgctgg tctctggtgc
    18361 aggcccgatt ggtattatgg cagcggcggt ggcgaaacac gttggtgcac gcaatgtggt
    18421 gatcactgat gttaacgaat atcgtctcga actggcgcgc aaaatgggta tcacccgtgc
    18481 ggtcaacgtc gctaaagaaa accttaatga tgtgatggca gaactgggca tgaccgaagg
    18541 ctttgatgtc ggtctggaaa tgtccggtgc gccgccagcg tttcgtacca tgctcgacac
    18601 catgaaccac ggcggtcgca ttgcgatgct gggtattcca ccgtccgata tgtctatcga
    18661 ctggaccaaa gtaatcttta aaggcttgtt cattaaaggt atttacggtc gtgagatgtt
    18721 tgaaacctgg tacaagatgg cggcgctgat tcagtcaggt ctggatctct cgccgatcat
    18781 tacccatcgt ttctctatcg atgatttcca gaagggcttt gacgctatgc gttcgggcca
    18841 gtccgggaaa gtaattctga gctgggatta acacgaacaa gggctggtat tccagccctt
    18901 ttgcctgagg ataatccgtt aaatatgtaa aatcctgtca gtgtaataaa gatatcgtaa
    18961 ttgcgctgat ctcttatata gctgctctca ttatctctct accctgaagt gactctctca
    19021 cctgtaaaaa taatatctca caggcttaat agtttcttaa tacaaagcct gtaaaacgtc
    19081 aggataactt cagaggtcgt cggtaattta tgatgaacag caccaataaa cttagtgtta
    19141 ttattccgtt atataatgcg ggcgatgatt tccgcacttg tatggaatct ttaattacgc
    19201 aaacctggac tgctctggaa atcattatta ttaacgatgg ttcaacggat aattctgttg
    19261 aaatagcaaa gcattacgca gaaaactatc cgcacgttcg tttgttgcat caggcgaatg
    19321 ctggcgcatc ggtggcgcgt aatcgtggga ttgaagtggc aacgggcaaa tatgtcgctt
    19381 ttgtcgatgc tgacgatgaa gtctatccca ccatgtacga aacgctgatg accatggcgt
    19441 tagaggacga cctcgacgtg gcgcagtgca acgctgactg gtgttttcgt gaaacgggag
    19501 aaacctggaa atccatcccc agcgatcgcc ttcgctcaac cggcgtatta accggcccgg
    19561 actggctgcg gatggggctt tcttcgcgcc gttggactca cgttgtctgg atgggggttt
    19621 atcgccgcga tgttattgtt aaaaataaca ttaaatttat tgccggatta catcatcagg
    19681 atattgtctg gacaacagaa tttatgttta atgcgctgcg tgcgcgatat accgagcaat
    19741 cgttatataa atattatctg cataatacgt cagtgagtcg gttgcataga caagggaaca
    19801 aaaaccttaa ttatcaacgt cactatatta agattactcg tctgctagag aaattaaatc
    19861 gaaattatgc cgacaaaatt acgatttatc cggaatttca tcagcaaata acttacgaag
    19921 cattgcgtgt ttgccatgcg gtgcgcaaag agccggatat tattacccgc caacggatga
    19981 ttgccgagat atttacttcc ggtatgtggt aatgactcca acttattgat agtgttttat
    20041 gttcagataa tgcccgatga c
//
LOCUS       DAESHI010000074        20041 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.075, whole genome shotgun sequence.
ACCESSION   DAESHI010000074 DAESHI010000000
VERSION     DAESHI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20041
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.075"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            192..296
                     /locus_tag="KW011_002762"
     CDS             192..296
                     /locus_tag="KW011_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HBH7661058.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            607..2805
                     /gene="speF"
                     /locus_tag="KW011_002763"
     CDS             607..2805
                     /gene="speF"
                     /locus_tag="KW011_002763"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HBH7661059.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2802..4121
                     /gene="potE"
                     /locus_tag="KW011_002764"
     CDS             2802..4121
                     /gene="potE"
                     /locus_tag="KW011_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HBH7661060.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4177..5817)
                     /gene="pgm"
                     /locus_tag="KW011_002765"
     CDS             complement(4177..5817)
                     /gene="pgm"
                     /locus_tag="KW011_002765"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HBH7661061.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5843..6388)
                     /gene="seqA"
                     /locus_tag="KW011_002766"
     CDS             complement(5843..6388)
                     /gene="seqA"
                     /locus_tag="KW011_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HBH7661062.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6573..7337
                     /gene="ybfF"
                     /locus_tag="KW011_002767"
     CDS             6573..7337
                     /gene="ybfF"
                     /locus_tag="KW011_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBH7661063.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7477..7770
                     /gene="ybfE"
                     /locus_tag="KW011_002768"
     CDS             7477..7770
                     /gene="ybfE"
                     /locus_tag="KW011_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HBH7661064.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7910..8440
                     /gene="fldA"
                     /locus_tag="KW011_002769"
     CDS             7910..8440
                     /gene="fldA"
                     /locus_tag="KW011_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HBH7661065.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8650..8736
                     /gene="uof"
                     /locus_tag="KW011_002770"
     CDS             8650..8736
                     /gene="uof"
                     /locus_tag="KW011_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HBH7661066.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8729..9175
                     /gene="fur"
                     /locus_tag="KW011_002771"
     CDS             8729..9175
                     /gene="fur"
                     /locus_tag="KW011_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HBH7661067.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9260..9586)
                     /locus_tag="KW011_002772"
     CDS             complement(9260..9586)
                     /locus_tag="KW011_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661068.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9636..11042)
                     /gene="chiP"
                     /locus_tag="KW011_002773"
                     /pseudo
     CDS             complement(9636..11042)
                     /gene="chiP"
                     /locus_tag="KW011_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
     gene            complement(11620..13284)
                     /gene="glnS"
                     /locus_tag="KW011_002774"
     CDS             complement(11620..13284)
                     /gene="glnS"
                     /locus_tag="KW011_002774"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HBH7661069.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(13543..15489)
                     /gene="nagE"
                     /locus_tag="KW011_002775"
     CDS             complement(13543..15489)
                     /gene="nagE"
                     /locus_tag="KW011_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HBH7661070.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            15822..16622
                     /gene="nagB"
                     /locus_tag="KW011_002776"
     CDS             15822..16622
                     /gene="nagB"
                     /locus_tag="KW011_002776"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBH7661071.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            16682..17830
                     /gene="nagA"
                     /locus_tag="KW011_002777"
     CDS             16682..17830
                     /gene="nagA"
                     /locus_tag="KW011_002777"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBH7661072.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            17839..19059
                     /gene="nagC"
                     /locus_tag="KW011_002778"
                     /gene_synonym="nagR"
     CDS             17839..19059
                     /gene="nagC"
                     /locus_tag="KW011_002778"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HBH7661073.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            19107..19859
                     /gene="nagD"
                     /locus_tag="KW011_002779"
     CDS             19107..19859
                     /gene="nagD"
                     /locus_tag="KW011_002779"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HBH7661074.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
BASE COUNT         4910 a         5065 c         5034 g         5032 t
ORIGIN      
        1 tcttaacgca aatataaaac acgaaattac aacatttaag taacaaaaca gcatctaata
       61 tggcgatatt cataattaat gatttattat ctggggtatg atcgtttttt gttgatcttc
      121 ttcacagatt atagccattt catggataga ataactctac cttcaactga cacagcaaga
      181 ggtaaaggta aatggaaaat aataaccgct taatgcctca tataaggcgg acaacccata
      241 tcatgaagtt tgcccatcgt aatagttttg actttcattt ctttaatgcc cgctagtctt
      301 ctgactaaag ggcaccccaa cgtacaggtc tccctgactt taagcattac aggttaatac
      361 ctgtattcct cggtgctcat atactgctaa cccttttaaa ctctaaataa ttcgagtcgc
      421 agcacttgca acttgaggta tgacgagtat agccagttac cgggctggtc tgggttattg
      481 catctgcaaa aagcaaacta ctgatttatt tatcagcggt ggagctttgc tttttttccg
      541 gcgtgatcga tttctccttt gagaaattga ggacctgcta ttacctgaaa taaagagatg
      601 aataaaatgt cagaattaaa aattgccgtt agccgttctt gcccggattg tttttccact
      661 catcgtgcat gcgtgaatat tgacgaaagt aattatattg acgttgccgc cattatttta
      721 tcagtcaatg atgttgaacg tggaaaactc gatgaaatag acgctactgg ctatggcatt
      781 cctgttttta ttgcaacgga aaatgaagaa cgtgtaccag cagaatatct gccacgtatt
      841 tctggtgtct tcgagcattg cgaatcacgg aaagagtttt atggacgtca gttagaaacc
      901 gctgctagcc attatgaaac gcaactgcgc ccgcccttct tccgcgcact ggtcgattat
      961 gtcaatcagg gcaatagcgc ttttgactgc ccgggtcatc agggaggtga gtttttccgt
     1021 cgtcatccgg cagggaatca gttcgtggag tacttcggag aaacgctgtt ccgttccgac
     1081 ttatgcaacg cagacgtagc aatgggcgat ctgctgattc acgaaggcgc gccatgcatt
     1141 gcacagcaac atgcggcaaa agtgtttaat gccgataaaa catacttcgt tctaaatggc
     1201 acttcatctt ctaacaaagt ggttttaaac gccctgctaa caccgggtga tctggtgctg
     1261 tttgatcgca ataaccacaa atctaaccac cacggagcgt tgctacaggc tggtgcaaca
     1321 ccggtttatc tggaaacggc acgtaacccg tatggcttta tcggtggcat tgatgcgcac
     1381 tgttttgaag aaagttacct gcgtgagctg atcgcggaag tcgcaccgca gcgggcaaaa
     1441 gaggctcgtc ctttccgcct cgctgtgatt cagttaggca cctacgacgg tacgatttat
     1501 aacgcccgcc aggtggtgga taaaattggt catctgtgtg actacattct gtttgactcc
     1561 gcatgggttg gctatgagca atttatcccg atgatggctg actgttcgcc gctgttgctg
     1621 gatcttaatg agaacgatcc gggtattctg gttacgcaat ccgtacataa acagcaggct
     1681 ggtttttctc aaacttcaca aatccataaa aaagacagcc acatcaaagg gcaacagcgt
     1741 tatgtaccgc acaaacgcat gaacaacgcc tttatgatgc acgcctccac cagcccgttc
     1801 tatccgctgt ttgccgcact ggatatcaac gccaaaatgc atgaaggtgt cagcggtcgt
     1861 aatatgtgga tggattgtgt ggtaaatggc attaatgccc gcaaactgat cctcgataac
     1921 tgtcagcata ttcgtccgtt cgtacctgaa ctggtggatg gtaaaccctg gcagtcgtat
     1981 gaaacagcgc aaattgcggt tgatctgcgc ttcttccagt ttgtaccagg ggaacactgg
     2041 cattcttttg aaggctatgc agagaatcaa tactttgtcg atccttgcaa actgttgctg
     2101 acaaccccag gtattgatgc acgtaacggc gaatatgaag cgttcggtgt acccgcgacg
     2161 attcttgcta acttcctgcg cgaaaatggc gtagtgccgg aaaaatgcga tcttaactcc
     2221 atcctcttcc tgctgactcc ggcagaagat atggccaaac ttcagcaact tgttgccctg
     2281 ctggtacgct tcgaaaaact gcttgagtcc gacgcgccat tagcagaagt gctgccttcc
     2341 atctacaaac agcatgaaga gcgctacgcc ggttataccc tgcgtcagtt gtgccaggaa
     2401 atgcatgatt tgtatgcccg acacaacgtg aaacaactgc aaaaagagat gttccgcaaa
     2461 gcgcacttcc cacacgtcag catgaatccg caagaagcca actacgccta tttacgcggt
     2521 gaagtggagc tggttcgtct gccggatgcc gaaggtcgta tcgctgccga aggggcgctt
     2581 ccttatcctc cgggtgtgct gtgtgttgtt ccgggtgaaa tctggggtgg tgctgttttg
     2641 cgttacttca gcgctctgga agaagggatc aacctgctgc caggttttgc accggagctg
     2701 cagggtgtct atatcgaaga acatgatggt cgtaagcaag tttggtgcta tgtcatcaag
     2761 cctcgtgatg cgcaaagcac cctgttgaaa ggggaaaaat tatgagtcag gctaaatcga
     2821 acaaaatggg cgtcgttcag ttaaccatac tgacgatggt caacatgatg ggctccggta
     2881 tcatcatgct gccgacaaag cttgccgaag ttgggacaat ttcaattatc tcctggctgg
     2941 tgacagccgt aggctcaatg gcactggcat gggcattcgc aaaatgcggt atgttcagcc
     3001 gtaaatcagg tggtatgggc ggttatgccg agtatgcttt cggtaaatcc ggtaacttta
     3061 tggcgaacta tacctacggc gtctcactgc tgattgctaa cgtcgcgatt gctatttcgg
     3121 cggttggtta cggcaccgaa ctgctcggcg cgagtttgtc gccagtgcag attggtcttg
     3181 cgaccatcgg ggtgctgtgg atttgtaccg tggctaactt tggtggtgcg cgcattaccg
     3241 ggcaaatcag tagcattacc gtgtgggggg tcattattcc ggtggtaggc ctgtgcatta
     3301 tcggctggtt ctggtttagc ccgacgcttt acgttgattc ctggaatccg catcatgcac
     3361 cgttcttcag tgcagtaggt tcttccatcg ctatgacgct gtgggctttt ctcggtctgg
     3421 agtctgcgtg tgcgaatact gatgtagtgg aaaacccgga acgtaatgtg ccaatcgcgg
     3481 tactcggcgg tacgttaggt gcggcggtga tttatatcgt ctccaccaac gtgattgcag
     3541 ggattgtgcc taatatggag ctggcaaatt caacggcacc atttggcctg gccttcgcac
     3601 agatgttcac accagaagtg ggtaaagtca ttatggcgct gatggtgatg tcctgctgcg
     3661 gttcgctgct tggctggcag ttcaccattg cccaggtgtt taagtcttca tctgatgaag
     3721 gctacttccc taaaattttc tcccgtgtaa ccaaagtgga tgcaccggtg cagggaatgt
     3781 tgaccattgt gattatccag agcgggttgg cgctgatgac gattagcccg tcgctaaaca
     3841 gtcagttcaa cgtgctggtt aacctggccg tggtgaccaa tatcattccg tatattctgt
     3901 caatggcggc attagtcatt attcagaagg tcgctaatgt gcccccctca aaagcgaaag
     3961 ttgcaaactt tgttgctttt gttggcgcga tgtatagctt ttatgcgctg tactcatccg
     4021 gggaagaagc catgctgtac ggttccatcg tgaccttcct tggttggaca ctgtatggtc
     4081 tggtatcacc acgctttgaa ctgaaaaata aacacggtta aaaaagcaaa gtaaaaaagg
     4141 gcgatcttgc gaccgccctt tttttattaa atgtgtttac gcgtttttca gaacttcgct
     4201 aacaatctca accgcttctt tctcaatctg cttgcgatgt tcttcaccga ggaagctttc
     4261 gcagtagatc ttatatgcgt cttccgtgcc tgacggacgc gcggcgaacc agccgttgtc
     4321 agtcatcact ttcagaccgc cgatagaagc accattgccc ggtgccgccg tcaggcgcgc
     4381 ggtgatcggg tcgcctgcca gggtgctggc gctcaccatt tccggagaca gcttagacag
     4441 cgccgctttt tgtgcggaag tcgcagctgc ctgcaaacgg ttgtagctcg gcgcaccaaa
     4501 gcgttttgcc agttcgttgt agtgttcctg cgggttctta ccggtgacag cggtgatttc
     4561 cgccgccagc agacacatga tgatgccgtc tttgtcggtg gaccacggcg tgccgtcgaa
     4621 acgcaggaag gaagcccctg cgctctcttc gccgccaaag ccgaagctgc cgtcgaacag
     4681 accatcaaca aaccatttga aacctaccgg gacttctacc agcttgcggc ccaagtcatt
     4741 gaccacacgg tcgatcatcg cagaagaaac cagcgtttta ccgacagcaa catctttgcc
     4801 ccactgcgga cgatgctgga acaggtagtt gatcgccacc gccaggtagt ggttcggatt
     4861 catcaaacct gccggagtga cgataccgtg acggtcataa tccgggtcgt tagcaaacgc
     4921 cagatcgaac ttatcacgca atgccagcag gcccgccatc gcacactcgg aggagcagtc
     4981 catacggatc gcgccgtctt tatcgaggtg cataaagcgg aaggtttgat cgacctgatc
     5041 gttaacgatg gtcaggttga ggttgtaata ctcgccaatt cgcttccagt attcgatacc
     5101 ggaaccaccc agcggatcaa cgcccagcgt caggcccgtt ttctgaatcg ccgccatatc
     5161 aacgatatcg gccagacctt ccacaaactg ctgcaccaga tcctgctctt tcacatgacc
     5221 ggatgccatc gcttcgtcga gggagatacg cttcacgcct ttcaggccat cggccatcag
     5281 tgcgttggca cgatcttcca ccactttagt gacgttggta tcagccgggc caccatttgg
     5341 cggattgtac ttgataccac catcttccgg cgggttatgg gagggtgtaa tcacgatacc
     5401 gtctgccagc gggccacctt ttttattatg aaccaggatg gcattggaaa cggcaggcgt
     5461 cggagtgaag ccattgtttt cctgcacaat gacatcaacg ccgttcgctg ccagcacttc
     5521 cagtacggaa atgaacgcag gctcggacag ggcgtgagta tctttaccca catagcaagg
     5581 gccagtgatg ccgtttttcg cacgttcttc agcaattgcc tgagcgatcg ccagaatgtg
     5641 cggctcgtta aagctgtggc gcgctgcact gccacggtga ccggaagtac cgaatttcac
     5701 cgcgtgctcc gcattccctg cttctggttt cagtacataa tattgcgccg tcagttgggc
     5761 gacgttaatc aaatcactct gttgtgcagg ttggcctgca cgattgtgga ttgccattgc
     5821 tttgtccttt gtctgcaacg ttttagatag ttccgcaaac cttctcaatc aattccgccg
     5881 ggaattgcat cgactgcatg atgtgttcga tcatgctgca tttacggccg gtgttggtgt
     5941 tggtgatcac ccaatacggc gtgcctggca catgtttcgg cttggtctga ttaccatttt
     6001 tcagcagcgt ttgttcatct gccgcaaagt aaacgcgtgt acgaccgtgc aacgattccg
     6061 ttgcttcggc aaacgcctgg gcgtcaagag aatatagtgt agacaacagc agcataaagc
     6121 gattgaccgc tcgcttttgc tctgcgtatt cgtcagaaag cagaagttca cgcattgcgc
     6181 gaaccttgtc tttaatcgtt ttgaccggct tcgcttcgac gatagcaggt gacgcaacgc
     6241 gaacctcttt cgtcaccgga gcagcaggct gtgatgcggc ggaaaatttc aacatacgcc
     6301 gtaaaatgtc ggatgcgctc tcgccgatat gcttagtgtg gctggcaata tagctgtaga
     6361 gttcatcatc aacttcaatc gttttcatct taatccagta cagtgtctta gctgaataca
     6421 agtcgccaag agtatatgaa taaatcccgg cagcggatag cgtcaagcct ggcttgcaga
     6481 aaaactcgga ataaacctta tttgttgcag ccctgtggca caatggtcaa cataataccc
     6541 taacccgaca acgcgaaaaa aagaactttg ccatgaaatt gaatatccgc gcgcaaactg
     6601 cacaaaacca gcacaataat tctcccatcg ttcttgtcca tggtctgttt ggtagcctcg
     6661 acaaccttgg cgtactggct cgcgatctgg taaacgatca caatatcatc caggttgata
     6721 tgcgtaacca cggtctttca ccgagagatc cggtaatgaa ttacccggcg atggcgcagg
     6781 atcttgttga tactctggat gcacagcaga tcgacaaagc aacatttatc ggtcactcca
     6841 tgggcggtaa agcggtaatg gcacttacgg cactggcccg cgatcgcatc gataaactgg
     6901 tggcgatcga tatcgcaccg gtcgactatc acgtacgccg tcatgatgag attttcgcgg
     6961 ctatcaacgc ggtcagtgaa tcggacgcac aaactcgcca gcaagcagca gcaataatgc
     7021 accagcatct taatgaagaa ggggtgattc agtttctgtt gaaatctttt gttgacgggg
     7081 agtggcgctt taacgtgccg gtattgtggg atcagtatcc gcatattgtg ggttgggaga
     7141 aaatcccggc atgggatcac cctgccctgt ttatccctgg cggcaattct ccgtatgtta
     7201 gcgagcagta ccgtgatgat ttactggcac aatttccaca ggcacgggcg catgtgattg
     7261 caggcgcggg tcactgggtc catgctgaaa aaccggatgc ggtattacgc gctatccgtc
     7321 gctatctcaa tgattaacgc aactgattaa aaacccagcg tccgcgtcgc tttccgcgcg
     7381 tgggcgttgg cgtgactcat cgcgctgatg tattatggcg cgctatccat ccgggcagag
     7441 gcctggctga ttgtttcccc cgaagtcacc aagatcatgg ccaaagaaca aacggaccgt
     7501 acgacattag atctgttcgc gcacgagcgt cgaccgggac gaccgaaaac taatccgctt
     7561 tcgcgcgatg aacagctgcg tattaataaa cgcaaccagc taaaacgcga caaagtacgt
     7621 ggccttaagc gtgtcgaact gaagctgaac gcggaagctg tcgaggcgct gaacgagctg
     7681 gcggagtcgc gcaatatgag ccgtagcgaa ctgatcgaag agatgttaat gcagcaactg
     7741 gcggcgctgc gtagtcaggg catcgtttaa atttccactt tcatgtagca cagtgtgcag
     7801 tcctgctcgt ttgctgattc cgcaagactg cctgttctgc tatgattgcc tttatccgtg
     7861 ggcaattttc cacccccatt tcaataagtt tcaagaggtt atttcactca tggctatcac
     7921 tggcatcttt ttcggcagcg acaccggtaa taccgaaaat atcgcaaaaa tgattcaaaa
     7981 acagcttggt aaagacgttg ccgatgtcca tgacattgca aaaagcagca aagaagatct
     8041 ggaagcttat gacattctgc tgctgggcat cccaacctgg tattatggcg aagcgcagtg
     8101 tgactgggat gacttcttcc cgactctcga agagattgat ttcaacggca aactggttgc
     8161 gctgtttggt tgtggtgacc aggaagatta cgccgaatat ttctgcgacg cattgggcac
     8221 catccgcgac atcattgaac cgcgcggtgc aacgatcgtt ggtcactggc caactgcggg
     8281 ctatcatttc gaagcatcaa aaggtctggc tgatgacgac cactttgtcg gtctggctat
     8341 cgacgaagac cgtcagccgg aactgaccgc tgaacgtgta gaaaaatggg ttaaacagat
     8401 ttctgaagag ttgcatctcg acgaaattct caatgcctga tgtgatgcgg cgtagactca
     8461 tgtctacgcc gtattaatag ataatgccaa tcaaaataat tgctacaaat ttgtaacttt
     8521 tgctgttgta cctgtacaat gtcccggtgt tcaagtggcc ttgccgttgt aaatgtaagc
     8581 tgtgccacgt ttttattaac aatatttgcc agggacttgt ggttttcatt taggcgtggc
     8641 aattctataa tgatacgcat tatctcaaga gcaaattctg tcacttcttc taatgaagtg
     8701 aaccgcttag taacaggaca gattccgcat gactgataac aataccgccc taaagaaagc
     8761 tggcctgaaa gtaacgcttc ctcgtttaaa aatcctagaa gttcttcagg agccggacaa
     8821 ccatcacgtc agtgcggaag atttatacaa acgtctgatc gatatgggtg aagaaattgg
     8881 tctggctacg gtatatcgcg tactgaacca gtttgacgac gctggtatcg tcacccgcca
     8941 caattttgaa ggcggtaaat ccgtatttga actgacacag caacatcacc acgatcacct
     9001 gatctgcctc gactgcggca aggttatcga atttagtgat gattccatcg aagcgcgtca
     9061 gcgtgaaatt gccgcaaaac atggcattcg cctgactaac cacagtctct atctttacgg
     9121 tcactgtgcc gaaggcgatt gccgcgaaga tgaacatgcg cacgaaggca aataagccag
     9181 cctgaatgag aaaaagccaa cctgcgggtt ggctttttta tgcaagggaa ataagaaaag
     9241 cgagagttac agctctcact tatttgttat tactgcgaat ttcctgccaa actttatcac
     9301 aatcggcttt caccgcctga tcatttccgg tttgcgttgc acgcagacac tgctgataat
     9361 ccagtacgcg gacactttcc tggtcggcaa aatgctgatg ttgtttctct ttcttcagca
     9421 cgtttaacac gctctggcag gcttcaattt tttctggcga accttgtgcg gtgttgatac
     9481 aggcgctgta tgcctctttc agacgactgt cttcctgtgg cgcggttgat tgcgcacaag
     9541 ctaccagccc cgatgccatt atggcgatga gaatcagttt tttcatagca aattcctcaa
     9601 acgtgcggca ggcacaaaac ctgccgcgtc gggcatcaga agatggtgaa tggtgcgatt
     9661 accataaatt tcacgtcacg ctcatcctgg aagatgttgc cgtaaccacc gccccagctt
     9721 gggatgtcgg agtggttgtc gtattcggtg aagtgcagtt tgaacatcgt gcctttggcg
     9781 cgaccgtcct gaatggtgta gaccgcatcc aggctgtatg cagactcttc aatagtccgg
     9841 tttttgtcgt agtacgcatc cggattgctc tgctaggtcg caggtttagc atcccatgcg
     9901 taaacgtagg aagcgccgat ggcgaagcct ggaagattcc agtttttcag gtcatacatc
     9961 gcaccgaaga agaccgcttt ttcgccgtta gcgttgaagt cggaacggtt atcccaccag
    10021 atatccaggc ggccgttgga ggaagcgtag gttggagtca tacgttgcag gaagtatccc
    10081 tgctgaccgt cagccttaac ccaggtgcct tcgaggcgca aatccactac gtcagccgcc
    10141 cggtaaccaa aggtcaacgc ttgcagccag gcggtgccgt cataaaggtc gttgacgctg
    10201 cgatcgtcaa ctttatcgcg cgtaccatag aactggtagc tggtggttaa cgggctaccg
    10261 gcgatatcaa atttgtagct ggctttggca aaatattgat cgatataccc ttccgcctga
    10321 ccaaatgccg cttccagtac gaagttattt ttgaagtcgt atttcgcccc aagggagtgc
    10381 agataatcaa ctttggtggt tttatcgttc tgataaaact catccatttc cagatgccac
    10441 ggcgctttgt attcgttggt ccacatgtag gagaaactca acgcaccagc atcgccgtaa
    10501 tcaaaattcg ccccggcttc cgccccctga taagtacctg gcataaagct ccagtgcggc
    10561 gctaacagcg tttgaccagt tggctgaatg taacctgccc tcgcccaaac cggaccgtat
    10621 ttaaatttgg ccgcagcttt atacaggctt ataccgcttt tgtcgccgga ccagtcttcg
    10681 tcataggctt tattactttt tgaaaacgcg atttcgttcg ggtgggagct gtcgccgttt
    10741 tccgccattt caatcgccgt aaacgcagca atatcaaggc cgaacatatc agcagcataa
    10801 ccggactgaa aatcgaggtt ggcattccag gtggagtgag aaaggttggt tttgtatttg
    10861 tcgccgtcgg taacatcttt acggtcgcgt tcacgctgcc agtaatatat accgccggtt
    10921 aaggttgaat cgtcgatgaa tccttcggcg cgagcctccg gcattgccat aaagcccgac
    10981 attgctgtaa caccggcgat agccagcgcc agcgtactat gtttgccact aaacgtacgc
    11041 atgggttaat cctctttgac gtataaattg ctgcaccaaa ggtgaacagc gaaaaaataa
    11101 aaattaaaaa agtagcgttg agaaaagtct cgctgactac aggaacagac tatttttcgc
    11161 gacgcgaatt atcaatctct ttctgtaacc agaatgtaag aacatgagca accgcacata
    11221 ttttgatgat ttttgttaca aggcttaagt ttgcggttga aaggcttgca gaagaataaa
    11281 agcgtgaaat tccagcaaga tgaaagcggt tacattttat ttaattatgt tttgttattt
    11341 taaatttatt ttatggataa attgcgaagc ggaattaatt cgcgaagaaa agcagaaaaa
    11401 acgccgctga acgcggcgtt ttggattgtt tgcatatttc ttgccggatg cggcgtgaac
    11461 gccttaaccg gcctacaaaa gcacgcaaac tcaatatatt gcagagatca tgtaggcctg
    11521 ataagcgtag tgcatcaggc aatttagcgt ttgaaactgt ttgtctgttc cttgccggat
    11581 gcgacgtaaa cgccccatcc ggcatagcga aacttaaaat tactcgccta ctttcgccca
    11641 ggtatcacgc agcccaacgg tgcggttaaa taccggtttt tccgccgtag aatggcggct
    11701 atcgaggcag aagtaacctt cacgctcaaa ctggaatgct ttacccgcaa ccgcatcttt
    11761 cagcgacggt tcagcaaagc cctgtttgat caccagcgat tccgggttaa tcaccgacag
    11821 gaaatcatcc gcagcaccag ggttcggcac gctgaacaga cggtcgtaca gacggatttc
    11881 aaccggcagc gcatgtgccg cgctcaccca gtgaataaca cctttgactt tacgaccatc
    11941 tgccggatct ttgcttaagg tgtcggcgtc ataagtacag aagatggtgg tgatattgcc
    12001 ttcggcatct ttctccacgc gttctgcctt aatgacgtaa gcattacgca gacgcacttc
    12061 tttacccagc accagacgtt tgtactgctt gttagcttct tcgcggaaat cggcgcgatc
    12121 gatccaaatc tcaccgctaa acggcacctg gcggctaccc atttccggtt tgttcggatg
    12181 gttcggcatg gtaaccattt cgccttcgcc ctgatagttt tcgataacca gtttcaccgg
    12241 atcgataacc gccattgcgc gcggggcgtt ttcgttgaga tcttcacgga tgcaggattc
    12301 cagcgacgcc atctcaatgg tgttgtcctg cttggtcacg ccgatgcgtt tgcagaactc
    12361 acgaatagaa gcagcagtgt aaccacgacg acgcagaccg gaaatggtcg gcatacgcgg
    12421 gtcatcccag ccttcaacgt gcttgtcggt caccagcagg ttcagcttac gcttggacat
    12481 cacagtgtat tccagattca ggcgcgagaa ttcgtactgg cgcgggtgaa caggaatggt
    12541 gatgttgtcc agcacccagt cgtacaggcg acggttgtcc tggaactcaa gcgtacacag
    12601 agagtgcgta ataccttcca gggcatcgct gatgcagtgg gtgaagtcgt acatcgggta
    12661 gatgcaccac ttgttgccag tctggtggtg ttcagcaaat ttaatgcggt acagcaccgg
    12721 atcgcgcatc acgataaacg gtgaagccat gtcgattttc gcacgcaggc aggctttacc
    12781 ttcttcaaaa ccaccggcac gcattttttc gaacagcgcc aggttctctt caacgctgcg
    12841 gtcgcggtac gggctgtttt tacccggttg cgtcagggtg ccgcggtatt cgcggatctg
    12901 ttccggcgtc agttcatcaa cgtacgccag gcctttattg atcagttcga tcgcataggc
    12961 gtggagctga tcaaaataat cggaggagta acggacgtta ccagaccagt gaaaacctaa
    13021 ccactctacg tcgtttttga tcgactcaac atactcgata tcttctttta ccgggttagt
    13081 gtcgtcgaaa cgcaggttgc actggccttt atagtcctgg gcgatcccga agttcaggca
    13141 gatagatttc gcatggccaa tatgcagata gccattcggc tccggcggga aacgggtgtg
    13201 tactgtggtg tgcttaccac tggccagatc ttcatcgatg atctgacgga taaagttagt
    13261 cgggcgggct tctgcctcac tcatcgtgga ttcctcaaag cgtaaacaac gtataacggc
    13321 gtatgatctt ataagcggga caggctgaca actgttagtt agcgaaaatc gaaaatctca
    13381 ctgagattat ggggatgatt gatgaaaaaa aaagcggcag agaagatctc caccgctttt
    13441 gccagtcccg cgtcattgtt ggatgcgcgt cttatccaac caggactgag cttttgtagg
    13501 cctgataaga cgcgtcaagc gtcgcatcag gcataaagca gattactttt tgatttcata
    13561 cagcggtgtt tgacccgcca caacattgcc ctgagcttta atgatcaagc cactgaaatc
    13621 gtcgatattg ctgcaaacca ccgggctaat catcgaacgg gcgttagcgt tcaggtaatc
    13681 cagatccatt tccagaatcg gttgccctgc gctcacctgc gcgccctctt ccaccagacg
    13741 tttaaagcct ttaccttcca gcgctacggt gtcgataccc atgtggacga caatctccgc
    13801 gcctttttcg gtttctaggc agaacgcgtg gttggtgttg aagattttca cgattgtccc
    13861 tgcggctggt gatacgacga ttttatctgt cggtttcacc gccacaccgt cacccaccgc
    13921 tttgctggcg aatgcttcgt cagaaacctg atccagtgcc acgacatcac cggtaatcgg
    13981 cgataccagc tccgcgatag ataccgcgtt tggtacagcc tgcggttttg ctacaggagc
    14041 ggcagttgcc ggagttgctt cagctgacgc agctgctacc gggccacggg caacgacttt
    14101 cttcatcgca tcgccaatgg attctgcttt cgcgccaaca atcacctgaa tggtctgttt
    14161 gttcagtttt actacaccag aagcacccag acgtttacac atcgcatcgt tgacgcgagc
    14221 tgagtcagcc acggtaaggc gcagacgggt aatacaggcg tcaatcgctt tcaggttgtc
    14281 agtaccgcca accgcagcaa tatagttggt tgccagttga gtcagacctt cttcagtatt
    14341 gctgttagcc tcttcagtta cgatctcgtc ttctttatct tcacgacccg gcgttttcag
    14401 gttgaacatg cggataacca aactgaacac cacgaagtag atagcgaaga agacaacacc
    14461 catcaccagc agcatccaga cgttctggct ggcggccggc aggttataca tcaacgcgta
    14521 gtcgatagcc cccgcagaga aagagaagcc agcatggata cccagcagcg ttgccacaaa
    14581 caggctgata ccggtcagca gtgcgtgcag gaggtacagc agcggagcaa ggaacatgaa
    14641 caggaattcc agcggctcag tcacaccggt caggaacgca gtaacagcaa cagaaagcag
    14701 catcccgcca accatcggac gacgctcttt cggtgctgcg aagtacatcg ccagcgccgc
    14761 acccggcaga ccgaacatca tgatcgggaa gaagccggac atgaacatcc ccgcggtgcc
    14821 gtcaccagcg tagaaacggt tgatgtcgcc gtggaaaacc gtacccgccg cgttggtgaa
    14881 ttcaccaatc tggaaccagg cgatggtgtt cagcacctga tgcagaccgg ttgggatcag
    14941 cagacggttg atgaaaccaa agataccgga acccagcgcg cctgcagaaa cgatccactc
    15001 gccgcctgca tggatagcgt gctgtactgg cggccagacg taaccaaaaa tggccgccag
    15061 caccaggcag aagaagccgg tggcaatcgg cacaaagcgt ttgccgccga agaagctcag
    15121 gaagtccggc agtttaatat cggaccaacg gttataggct gcgccaccaa ccagaccggt
    15181 aatgataccc gccagtacac ccatgttaat ttctgggttg atggtcacca tcgctttggt
    15241 taacacaaag taacctaccg cacccgccag cgccgccgca cctgcgctgt ctttcgacca
    15301 gctggatgcc acaccgatgg cgaagattaa tgcgaggtta tcaaaaatcg caccgcccgc
    15361 ctgggcaata aacgcaacgt taagtaaatc tggctgaccg aatcgcagca acagtgccgc
    15421 caccggcagc accgcgatag ggagctgtaa cgccctaccg agtcgctgga aaaaacctaa
    15481 aatattcatc ttattccccc tacgagaacc ctatttggct cgtttaaagc cgtattttta
    15541 ttttgctgca aattgtactg ccgatgttct gtaatcagat tgttagatca tctgctacag
    15601 agtgtgtgaa aatttaattc gtatcgcaaa ttaaacgcgt gccttttgtg agttttgtca
    15661 ccaaatatcg ttatcatcac tcccttttac tggctaaagc agaaaactta ttttatcatt
    15721 caaaaaatca ggtcggattg acgcctgtct gcgcaaatcc aggttacgct taaagatgcc
    15781 taatccgcca acggcttaca ttttacttat tgaggtgaat aatgagactg atccccctaa
    15841 ctaccgctga acaggtcggc aaatgggctg ctcgccatat cgtcaatcgt atcaatgcgt
    15901 tcaaaccgac tgccgatcgt ccgtttgttc tgggcctgcc gactggcggc acgccgatga
    15961 ccacctataa agcgttagtc gaaatgcata aagcaggcca ggtcagcttt aagcacgttg
    16021 tcaccttcaa catggacgaa tatgtcggtc tgccgaaaga gcatccggaa agctactaca
    16081 gctttatgca ccgtaatttc ttcgatcacg ttgatattcc agcagaaaac atcaaccttc
    16141 tcaacggcaa cgccccggat atcgacgccg aatgccgcca gtatgaagaa aaaatccgtt
    16201 cttacggaaa aattcatctg tttatgggcg gtgtaggtaa cgacggtcat attgcattta
    16261 acgaaccggc gtcttctctg gcttctcgta ctcgtatcaa aaccctgact catgacactc
    16321 gcgtcgcgaa ctctcgtttc tttgataacg atgtcaatca ggtgccaaaa tatgccctga
    16381 ctgtcggtgt tggtacattg ctggatgccg aagaagtgat gattctggtg ctgggtagcc
    16441 agaaagcact ggcactgcag gctgcagttg aaggttgcgt gaaccacatg tggaccatca
    16501 gctgtctgca actgcatcca aaagcgatca tggtgtgcga tgaaccttcc accatggagc
    16561 tgaaagttaa gactttaaga tatttcaatg aattagaagc agaaaatatc aaaggtctgt
    16621 aattgttatc cctgccctgg ctccttgctc agggcaatat tttttaaaat cggggggcag
    16681 aatgtatgca ttaacccagg gccggatctt taccggccac gaatttcttg atgaccacgc
    16741 ggttgttatc gctgatggcc tgattaaaag cgtctgtccg gtagcggaac tgccgccaga
    16801 gatcgaacaa cgttcactga acggggccat tctctccccc ggttttatcg atgtgcagtt
    16861 aaacggctgc ggcggcgtac agtttaacga caccgctgaa gcggtcagcg tggaaacgct
    16921 ggaaatcatg cagaaagcca atgagaaatc aggctgtact aactatctgc cgacgcttat
    16981 caccaccagc gatgagctga tgaaacaggg cgtgcgcgtt atgcgcgagt acctggcaag
    17041 acatccgaat caggcgttag gtctgcatct ggaaggtccg tggctgaatc tggtaaaaaa
    17101 aggcacccat aatccgaatt ttgtgcgtaa gcctgatgcc gcgctggtcg atttcctgtg
    17161 tgaaaacgcc gacgtcatta ccaaagtgac cctggcaccg gaaatggttc ctgcggaagt
    17221 catcagcaaa ctggcaaatg ccgggattgt ggtttctgcc ggtcactcca acgcgacgtt
    17281 gaaagaagcg aaagccggtt tccgcgcggg gattaccttt gccacccatc tgtacaacgc
    17341 gatgccgtat attaccggtc gtgaaccggg cctggcgggc gcgatcctcg acgaagctga
    17401 catttattgc ggtattatcg ctgatggcct gcatgttgat tacgccaaca ttcgtaacgc
    17461 taaacgcctt aaaggcgaca aactgtgtct ggttaccgac gccaccgcgc cagcaggtgc
    17521 caacattgaa cagttcattt ttgcgggtaa aacaatatac taccgtaacg gactttgtgt
    17581 ggatgagaac ggtacgttaa gcggttcatc cttaaccatg attgaaggcg tgcgtaatct
    17641 ggtcgaacat tgcggtatcg cactggatga agtgctgcgt atggcgacgc tctatccggc
    17701 gcgtgcgatt ggcgttgaga aacgtctcgg cacactcgcc gcaggtaaag tagccaacct
    17761 gacctcattc acacctgatt ttaaaatcac caagaccatc gttaacggta acgaggtcgt
    17821 aactcaataa gagaaagtat gacaccaggc ggacaagctc agataggtaa tgttgatctc
    17881 gtaaaacagc ttaacagcgc ggcggtttat cgcctgattg accagtacgg gccaatctcg
    17941 cggattcaga ttgccgagca aagccagctt gcccccgcca gcgtaaccaa aattacgcgt
    18001 cagcttatcg aacgcgggct gatcaaagaa gttgatcagc aggcctccac cgggggccgc
    18061 cgcgctatct ccatcgtcac cgaaacccgc aatttccacg caatcggcgt acggcttggt
    18121 cgtcacgacg ccaccatcac cctgtttgat ctcagcagca aagtgctggc agaagaacat
    18181 tacccgctgc cggaacgtac ccagcaaacg ctggaacatg ccctgttgaa tgccattgct
    18241 cagtttattg atagctacca gcgcaaactg cgcgagctga tcgcgatttc ggtgatcctg
    18301 ccagggcttg ttgacccgga cagcggcaaa attcattaca tgccgcatat tcaggtagaa
    18361 aactgggggc tggtagaagc tctggaagaa cgttttaaag tgacctgttt cgttggtcac
    18421 gatatccgta gtctggcgct ggcggagcac tacttcggtg caagtcagga ttgcgaagac
    18481 tccattctgg tgcgtgtcca tcgcggaacc ggggccggga ttatctctaa cgggcgcatt
    18541 tttattggcc gcaacggtaa cgtcggtgaa attggccata ttcaggtcga accgctgggt
    18601 gaacgctgcc actgcggcaa ctttggctgc ctggaaacta tcgccgccaa cgctgccatt
    18661 gaacaacggg tgttgaatct gttaaagcag ggctaccaga gccgcgtgcc gctggacgac
    18721 tgcaccatca aaactatctg caaagccgcg aacaaaggcg atagtctggc gtcggaagta
    18781 attgagtatg tcggtcgtca tctgggtaaa accatcgcca ttgctatcaa cttatttaat
    18841 ccgcaaaaaa ttgttattgc cggtgaaatc accgaagccg ataaagtgct gctccctgct
    18901 attgaaagct gcattaatac ccaggcgctg aaggcgtttc gtactaatct gccggtggta
    18961 cgttctgagc tggatcaccg ctcggcaatc ggcgcttttg cgctggtaaa acgcgccatg
    19021 ctcaacggta ttttgctcca gcatttgctg gaaaattaat gtgcttttat agtggcgctt
    19081 attgttgtca atattctggg tagtccatga ccattaaaaa tgtaatttgc gatatcgacg
    19141 gcgtgctgat gcacgataac gtcgccgtac cgggtgcagc ggaatttttg cacgggatta
    19201 tggataaagg cctgccgctg gtgttgctga ccaactatcc ttcgcagacc gggcaagatc
    19261 tggcgaaccg ctttgccact gccggtgtcg atgtgccgga tagcgtgttt tatacctctg
    19321 cgatggcgac cgccgatttc ctgcgtcgcc aggaaggcaa gaaagcatat gtggtgggcg
    19381 aaggcgcact gattcatgaa ctgtacaaag ccggtttcac tattaccgat gtgaaccctg
    19441 atttcgtgat tgttggcgaa acgcgttcct acaactggga catgatgcat aaagcggcct
    19501 atttcgtcgc taacggtgca cgctttatcg ccaccaaccc ggacacccac gggcgcggtt
    19561 tttatcccgc ttgtggcgcg ttgtgtgcag ggattgagaa aatctccggg cgcaaaccgt
    19621 tctatgttgg taagcccagc ccgtggatca tccgcgcagc attaaacaaa atgcaggcgc
    19681 attcggaaga aacggtgatt gtcggcgata acttgcgcac cgacattctg gcgggcttcc
    19741 aggcaggtct ggaaacgatt ctggtgcttt ctggtgtttc atcgctcgac gatatcgaca
    19801 gtatgccgtt ccgcccaagc tggatttacc cgtcagtcgc tgaaatcgac gttatctgaa
    19861 taccacccac gtctgcggac gtgggtggct ctgatgacaa acgccaaact gcctgatgcg
    19921 ctacgcttat caggcctacg cagctcctgc aatatattga attttcatgc ttttgtaggc
    19981 cggataaggc gttcacgccg catccggcat gaacaaatcg cactttatca gcaatttggc
    20041 c
//
LOCUS       DAESHI010000075        19763 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.076, whole genome shotgun sequence.
ACCESSION   DAESHI010000075 DAESHI010000000
VERSION     DAESHI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19763
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.076"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..369
                     /locus_tag="KW011_002780"
     CDS             <1..369
                     /locus_tag="KW011_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBH7661075.1"
                     /translation="AMMYAETRNMTPYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYV
                     YSKGKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYFNKNFSTYVDYKINLLDKDDDF
                     YDNNGIATDDVVGVGLVYQF"
     gene            complement(509..704)
                     /locus_tag="KW011_002781"
                     /pseudo
     CDS             complement(509..704)
                     /locus_tag="KW011_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353128.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            960..1811
                     /gene="hchA"
                     /locus_tag="KW011_002782"
     CDS             960..1811
                     /gene="hchA"
                     /locus_tag="KW011_002782"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HBH7661076.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            complement(1919..3277)
                     /gene="hprS"
                     /locus_tag="KW011_002783"
     CDS             complement(1919..3277)
                     /gene="hprS"
                     /locus_tag="KW011_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HBH7661077.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(3277..3948)
                     /gene="hprR"
                     /locus_tag="KW011_002784"
     CDS             complement(3277..3948)
                     /gene="hprR"
                     /locus_tag="KW011_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HBH7661078.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            4081..4494
                     /gene="hiuH"
                     /locus_tag="KW011_002785"
     CDS             4081..4494
                     /gene="hiuH"
                     /locus_tag="KW011_002785"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HBH7661079.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            4603..5607
                     /gene="msrP"
                     /locus_tag="KW011_002786"
     CDS             4603..5607
                     /gene="msrP"
                     /locus_tag="KW011_002786"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HBH7661080.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            5608..6243
                     /gene="msrQ"
                     /locus_tag="KW011_002787"
     CDS             5608..6243
                     /gene="msrQ"
                     /locus_tag="KW011_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HBH7661081.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            6500..7150
                     /gene="zinT"
                     /locus_tag="KW011_002788"
     CDS             6500..7150
                     /gene="zinT"
                     /locus_tag="KW011_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HBH7661082.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            7493..8023
                     /locus_tag="KW011_002789"
                     /pseudo
     CDS             7493..8023
                     /locus_tag="KW011_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(8593..8682)
                     /locus_tag="KW011_002790"
     tRNA            complement(8593..8682)
                     /locus_tag="KW011_002790"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8646..8648),aa:Ser,seq:cga)
     gene            8776..9573
                     /gene="mtfA"
                     /locus_tag="KW011_002791"
     CDS             8776..9573
                     /gene="mtfA"
                     /locus_tag="KW011_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HBH7661083.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            9674..9749
                     /locus_tag="KW011_002792"
     tRNA            9674..9749
                     /locus_tag="KW011_002792"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9707..9709,aa:Asn,seq:gtt)
     gene            10063..17128
                     /gene="yeeJ"
                     /locus_tag="KW011_002793"
                     /pseudo
     CDS             10063..17128
                     /gene="yeeJ"
                     /locus_tag="KW011_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
     gene            complement(17390..18442)
                     /locus_tag="KW011_002794"
     CDS             complement(17390..18442)
                     /locus_tag="KW011_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HBH7661084.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCYSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            18757..>19763
                     /gene="shiA"
                     /locus_tag="KW011_002795"
     CDS             18757..>19763
                     /gene="shiA"
                     /locus_tag="KW011_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HBH7661085.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MA"
BASE COUNT         5513 a         4657 c         4409 g         5184 t
ORIGIN      
        1 gcaatgatgt atgctgaaac ccgcaatatg acaccatatg gtaatgtggg tatcgcaaat
       61 aaaacccaga actttgaagc tgtagcacag tatcagttcg attttggtct gcgcccatct
      121 cttgcatatg tttattcaaa aggcaaagac ctcggcggta acgactataa caacaatggt
      181 catcaagagt atgttgatca ggatctggtc aactatgttg aaattggagc gacctactat
      241 ttcaataaga acttctcaac atatgttgac tataaaataa acttgttgga taaagatgac
      301 gatttctatg acaacaatgg catcgcaaca gatgacgttg ttggtgtggg cttagtctac
      361 cagttctaat cagccaacgt gctgcggcgt gttactgtaa aactaaacac tagcctgagt
      421 tcgtccctca tcgaattcag gcttttttat tggcatttca tctccatctg cggcacacgc
      481 aattgccaat gcatatcgat acagcacctt accctgcccc tcctggcaca ataacatcaa
      541 ccagcgactc ttcaaaccaa cacccaaatg catttcttta tgaattaatc cgtaacaaac
      601 acgcttccca tatagaaata tttgcaaaaa tagtgcatga aataatcttt tctctaccaa
      661 tccgcgatat aaatattcat cagtaccaca atatttcaca gcactaaatc tctccccgcc
      721 gccccgtacc tttgataatg gtctaaaatc attgaggcca cttgcgacga cgttgctcaa
      781 taaggaaaat ttggcgtgaa taatattcaa caccagtgca gcatcatcat tacatactta
      841 cacccgccga ctaccgcttt cgatatattg actcaatttt ccagactggt taaaaatagc
      901 gaccagctca gtcgcaaata tagtgactac cctaactaag caacaataag gaatacacta
      961 tgactgttca aacaagtaaa aatccgcagg tcgatattgc tgaagataat gcattcttcc
     1021 cttcagaata ttcgcttagc caatatacca gtcccgtctc tgatcttgat ggcgtggact
     1081 atccaaaacc gtatcgcggt aagcataaaa ttctggtgat cgccgcggac gaacgttatt
     1141 tgccaaccga taacggaaaa ctgttctcga ccggtaacca tccgattgaa acgttgctgc
     1201 cgttgtatca tctccatgct gctggtttcg aattcgaagt ggcgaccatt tccggtctga
     1261 tgaccaagtt tgaatactgg gctatgccgc acaaagatga aaaagtaatg ccattctttg
     1321 agcagcataa atcgttgttc cgcaatccga agaaactcgc ggatgttgtc gccagcctca
     1381 acgctgatag cgaatatgca gcaatctttg ttcctggtgg tcatggcgca cttattggtt
     1441 taccagaaag ccaggacgta gctgctgctt tgcagtgggc aatcaaaaat gaccgttttg
     1501 ttatctccct ttgccacggc ccggcggctt ttctggcgct tcgccacggc gataacccac
     1561 tgaatggtta ttccatttgc gcattcccag acgccgcaga caaacaaacg ccagagatag
     1621 gctatatgcc gggtcatctc acctggtact tcggcgaaga gctgaagaaa atgggcatga
     1681 acattattaa tgacgacatc accgggcgag tacataagga ccgtaaagtt ctcaccggcg
     1741 acagtccttt cgcagcgaat gcgttgggta aactggcggc gcaggaaatg ctggcagctt
     1801 atgcggatta atccctgtta cgtttgaatg taaacgcata atcaatcgca gccacgttaa
     1861 tactcacgac gtggctgcgt tttttcatgg ttgatttatg gcgtactgaa gccctatgtt
     1921 aatttctttg cggtaacata atccggaaca cattatgctt attgagatag tgatacgtag
     1981 cacttccccc atgtaattcg gcaatcgctt tgactaaaga aagaccaagt ccctgaccta
     2041 cggaatgacg cgaattatct ccccgccaaa atctacggaa gagtttttca ggctcatgaa
     2101 ttttcgttcc aggactggcg acatcaatat taagatagcc gttggtatca agaaaactgg
     2161 ttatatgaat acgcgatttt tctggtgaat atctaatggc attaacaata agattcgata
     2221 acattcgttg cagtaaaatt ttatccgcaa agatttgctg attgcactcg accttaaagc
     2281 aaatctcttt ctcgtctgaa aggtattcaa gataatccaa caaattttcg acttccttat
     2341 tgagagaaag cgagtccagt ttcaccaaaa cattgttttt atctgcccgg gcaagaaaca
     2401 gtatgttctc tgttaaccgc gaaatatttt ccagctcttc aatgttaccg gcaattgttt
     2461 tttgatattc agcgatactt ctggtttgac tgagcgtaac ctgattctga cccagtaatg
     2521 cattaatggg cgttctaagt tcatgagcga gatcgtcagc aaattgactt agacgctcaa
     2581 aatctttgac taaagcatga tgcattttat tcaacgcctg cccaagaggt tttagttctc
     2641 tcggtaatgc gctaacctca acaggctccc ggctatcgtt ataattcagc gcttccgtta
     2701 caccactcaa ctttttgatc tctcgtaatc ccgttctgat taacagcgga cttaatactg
     2761 agcaaagtac aatggcgaca atgcaaatta taatgctatt aattttatac tgttcaagca
     2821 tgttatgtct ggctgaagcc aatttagcca cagtaaccgt taatgatggc gaaacttcat
     2881 caatattaat tcgtaaagca tcaatctcag tatcattaat aatgcttctg taaatgccag
     2941 ctgcgctgat tgtctcacta gcaggtatgt tatttaacat gtcatcactg acatttgtcc
     3001 ggttaacaat tttattgatg ccatcaccat gaatgatcaa gatatcctga ctaacatcca
     3061 tcatccggtt aaagtataca ggtaacgtat ctggatttac cccatcaatt aacaactgct
     3121 tgatctgtgc tgtccggtta atgagtgttg tatcatcgcg ccatttcaac tcacttgcca
     3181 ggccattata gagagtccag acaattccag cgccagcaac agacagcagc aatataaaaa
     3241 gcaaggttaa acggacggtt atagataatc ttttcattat ttttttaccg ctacgaatga
     3301 atagcccatc ccccggattg tggcgattag cttttcagga aaaggatcat caacttttgc
     3361 gcggagcctg cgaatggcga catccaccgt attggtatca ctatcaaagt tgattcccca
     3421 aatttcactc gcaataaccg ttctgggtat aatttcgcca gctctggagg ccagtagcca
     3481 aagtaactga aactccttgc gcgtcagtgt aatactgata ttgttcctgc tcacactttg
     3541 acttacagag tccattctta agccactgat ttctaatgtt gaattcaaag cgtgatgttg
     3601 ccttaattgt gcccgaaccc ttgccagcaa ctcagaaaat gaaaaaggtt ttaccagata
     3661 atcatttgct ccactgtcca gccctctgac tctgtcatcg acagaatccc ttgcagtaag
     3721 gcaaataaca ggggtttgct ttgctgttct taacgtttgt aagatctgcc agccatccat
     3781 acccggaagc ataatatcca gaatgatcaa tgcataatca tccttcagcg caagataaag
     3841 cccatctctg ccatcagaaa cggcatcaat gacataaccc gcttcggaaa gcccctgcgt
     3901 tacccactcc tgggtccttt gattatcttc aataagtaaa atcttcatga aaataatatg
     3961 ccataaatca tatgttatag aaacagccta gttcattaca aaattgtaat gctgctgtaa
     4021 ggttaccctg gccgcttttt cgctatcctc aaaactcatt cacatgacaa ggatataaac
     4081 atgttaaagc gttatttagt actctccgta gtaacggcag cattttcatt accttctttg
     4141 gtttatgccg cacaacaaaa cattcttagc gtgcacattt tgaaccagca aaccggaaaa
     4201 cccgctgccg acgtgacagt cactcttgaa aagaaagcgg acaacggctg gttacaactt
     4261 aataccgcca aaacagataa ggatggacga attaaggcac tgtggcccga gcaaactgca
     4321 actacgggcg attaccgtgt cgtatttaaa accggggact atttcaagaa acaaaatctt
     4381 gaaagtttct tccctgagat ccccgttgaa tttcatatta ataaagtgaa cgagcattat
     4441 catgtgcctt tattacttag ccaatatggg tattcaacct atcgtggcag ttaatgcata
     4501 aatatgtaaa agcagatctc tgctactgtt agggaggaca ctccctgaca gattaacagt
     4561 aaacggctct tgctggctaa cgacaaaaaa gtgtgatggc ttatgaaaaa gaatcaattt
     4621 ttaaaagaat cagatgttac ggccgagtcg gtattcttta tgaagcgtcg acaggtgtta
     4681 aaagcactgg gcatcagcgc agctgcactt tctttgcctc acgctgcgca tgccgatctg
     4741 cttagctggt ttaaagggaa cgatcgcccg cccgcccccg ccggaaaacc gctggagttc
     4801 agcaagccta ccgcctggca aaataatctg ccactgacgc cagtagataa agtctccggt
     4861 tataacaact tctatgaatt cgggctggat aaagccgatc ccgccgctaa tgctggtagc
     4921 ctgaaaaccg atccatggac actgaaaatc agcggcgaag tggcaaaacc attgaccctc
     4981 gatcacgatg atttaacccg tcgcttcccg ctggaagagc gtatttatcg tatgcgctgc
     5041 gtggaggcat ggtcgatggt ggtgccgtgg attggttttc cgctgcacaa attgctggcg
     5101 cttgccgaac ccaccagcaa tgcgaagtat gtcgctttcg aaacaattta tgcaccggaa
     5161 caaatgccag gccagcagga ccgctttatc ggcggcgggc tgaaatatcc ttatgtcgaa
     5221 ggattgcgtc tcgacgaagc aatgcatccg ctcacactga tgaccgtggg tgtttatggc
     5281 aaggcgttac cgccacaaaa tggcgcgccg gtacgactga ttgtgccgtg gaaatatggc
     5341 tttaaaggga ttaaatcgat agtcagtatt aagctgaccc gcgagcgtcc gccaaccacc
     5401 tggaatctgg cagcgcctga cgaatacggt ttttacgcca acgttaatcc gcatgttgat
     5461 cacccgcgct ggtcacaggc taccgaacga tttattggtt caggcggcat cctcgatgtt
     5521 cagcgccagc caacgctact gtttaatggt tacgccgacc aggtggcatc gctgtatcgt
     5581 ggcctggatt tgcgggagaa tttctgaatg cgtctgacag caaaacaggt gacatggctg
     5641 aaagttagcc tgcatcttgc cggattgttg ccgtttctct ggctggtctg ggcgatcaat
     5701 cacggtggac tgggtgccga tccggtgaaa gatattcagc attttaccgg tcgcactgca
     5761 ctgaaatttt tgctggcgac cttgttaatc acacctctgg cacgctacgc aaaacagccg
     5821 ttattgatac gcactcgccg cctgttaggc ttatggtgct ttgcctgggc gacactgcat
     5881 ttaaccagtt acgcattgct ggagctgggc gtgaataatc tggcgttatt aggcaaggag
     5941 ttaattaccc ggccttattt aacgttagga attatcagct gggttatttt gcttgcttta
     6001 gcgtttactt caacccaggc gatgcagcga aaactcggca agcactggca acagttgcat
     6061 aatttcgtct atcttgtcgc gatcctggcc ccaatacatt atctgtggtc agtgaagatt
     6121 atttcaccgc agccgctcat ctacgctggg ctggctgtac tgcttttagc cttacggtat
     6181 aagaagttgc gttccctttt taagcggtta cgcaagcagg ttcacaacaa attatctgta
     6241 taacttcata ttgccgacaa agtacgcgtt atccctgccg gatgtggcgc gagcgccctg
     6301 tccggcctgc gcagatcaca tcaggttatg tttcgtttgt catcaacctc aaattatcaa
     6361 cataattcat tttatttcaa taagatagat aagtacacct gagaaaacta tgctctcatc
     6421 tcctaagagt tgttgcattt tgccatatgt tacaatataa cattacacat catatacaat
     6481 gactctggag gaaactgttt tggcaattcg tcttcacaaa ctggctgttg ctttaggtgt
     6541 ctttattgtt agcgctcctg ccttttcgca tggtcatcac tcacacggca aacccttaac
     6601 agaggtcgaa caaaaagctg ctaatggtgt ttttgatgat gccaatgtac aaaaccgaac
     6661 gctcagtgac tgggatggag tctggcaatc cgtttatcct ttactgcaaa gtggcaaact
     6721 tgaccccgtc tttcagaaga aagcggatgc agataaaatt aaaacatttg ctgaaattaa
     6781 agattattat cacaaaggtt atgcaacaga tatcgagatg attggcattg aggacggcat
     6841 tgttgaattc catagaaata atgaaacaac atcctgtaaa tatgattacg atggatacaa
     6901 aatactcacc tataaatcag gcaagaaagg cgttcgttac ttattcgaat gtaaagatcc
     6961 tgaaagcaaa gcccctaaat atatacaatt tagcgatcat ataattgcac cacgaaaatc
     7021 atctcatttt cacattttta tgggtaatga ttcacagcaa tttttattaa atgaaatgga
     7081 aaactggcca acgtattatc catatcagtt gagtagcgaa gaagtggtcg aggaaatgat
     7141 gtctcattga gtattctcat gataacgcct cgatgccgct ttagtaagtc atcataactg
     7201 ccactggtcc atccacaaac gccactgaac gcaagctagc tacagacacg ctcatcacta
     7261 tgacgtgtct gtatattaat aagctaaccc gcattgagtt aaccaataac ggattccata
     7321 cacaatacgg ccaacagaga aagatacctg tgctcacgcc attgcttata ttggctggtt
     7381 acaatgtgca ctatcaattt tttaaataac aaattcttaa aaccacatct gtataaggaa
     7441 tttttaaggt tcgtgggata gcttgactgt aaaaattaca ggagctacaa aaatgaaccg
     7501 attctcaaaa actcaaattt atttacattg gataacgctg cttttcgttg caataaccta
     7561 tgccgcgatg gaactccgtg gctggtttcc taaaggtagt agtacttatc tgctgatgcg
     7621 agaaacacat tacaatgcgg gtatattcgt ttgggtgtta atgttttcac gcctgattat
     7681 aaaacaccgt tatagtgatc cttctattgt accaccgcca cctgcctggc aaatgaaagc
     7741 ggcttcgcta atgcacatca tgctttatat aaccttcctt gcacttcctc tgctggggat
     7801 tgctttgatg gcttacagtg gaaaatcgtg gagtttcctt ggtttcaatg tgtctccctt
     7861 tgttacccca aacagcgaaa ttaaagcact gataaaaaat attcacgaaa cctaggcaaa
     7921 tataggttac tttttaatcg cagctcacgc tggcgcagca ctctttcatc actacattca
     7981 gaaagataat actctgttac gaatgatgca tcgccgcaaa taagtcgatc ccctgacaag
     8041 gaaatgtgaa ccatgtacat caacgcgtca taaatgttta cgcaagtgca caatgcatcc
     8101 tttctactat caacaaggag gagcagaaac tgtgtaaatc gtacaatggt aagacttatc
     8161 aaaaatacag gttaattttg gtatttaaac cttttttact tgttagtatg atacaaaggc
     8221 tttcaaaaaa gctgggaaat tcaacaactc ttggaaatta tggactttgt cccgcggctt
     8281 taatctgacg gccgcgttcc ttttttgact caattattga ccccttctct acgcaacttc
     8341 agttcccacc accaactttg cggcagtttc ataggatgaa tgtctaaaag aataatggtg
     8401 agcgataaga aaacgactga ataactgcac attttcgctc gaaagcttcc tgtcagatcc
     8461 atagcgaatc aagtgctgaa tgtcacagta tcgaacagaa aacagtgacg atctaaccct
     8521 tcaagaatat tctacaattg ttctgtttag gaaaagcaag gcgggaagtc aggagataag
     8581 tcattgataa agtggcggag agagggggat ttgaaccccc ggtagagttg cccctactcc
     8641 ggttttcgag accggtccgt tcagccgctc cggcatctct ccgttcagat ggttgccatg
     8701 atgccaggaa atttggcatt ttaacagtcc ctgtccgtgc aattttgttc aagtgacgag
     8761 tttgcgagca aaacgatgat taagtggccc tggaaagtac aagaatcagc acatcaaact
     8821 gcccttccct ggcaggaagc actatcgatc ccccttttaa cgggtctgac ggaacaggaa
     8881 caaagcaaat tagtcactct tgccgaacgt tttttacagc aaaagcggct tgttccttta
     8941 cagggctttg aactggattc attaagaagc tgccggatag cacttctatt ttgcctaccc
     9001 gttctggagt taggactgga atggctggat ggttttcatg aagtcttaat ttatcctgcg
     9061 ccatttgtgg tcgatgatga atgggaagac gatatcggtc tggtgcataa ccaacgtatt
     9121 gttcagtcag gtcagagctg gcagcaaggg cctatcgttt tgaactggtt ggatatacaa
     9181 gattcttttg atgcatctgg ttttaacctg attattcatg aagtcgctca taagctggac
     9241 acccgtaacg gcgatcgcgc cagcggagtt ccctttattc cgttgcgtga ggttgctggc
     9301 tgggaacacg atcttcatgc tgcaatgaac aacattcagg aagaaatcga attggttggc
     9361 gagaatgcgg cgagcattga tgcttatgca gccagcgatc ctgctgaatg ttttgccgta
     9421 ctttctgaat atttctttag cgccccagaa ctttttgctc ctcgtttccc ttcattgtgg
     9481 caacgtttct gccaatttta tcaacaagat cctttgcaga gactgcatca cgctaatgat
     9541 acagactcgt tttcggcgac gaatgttcat taattaacaa ctttgcagat taattaacca
     9601 attgaaatga cttatgaaat ttagtgttga cagacaaggt accgctaagt aatatgtgcc
     9661 ccgttcacac gattcctctg tagttcagtc ggtagaacgg cggactgtta atccgtatgt
     9721 cactggttcg agtccagtca gaggagccaa attctaaaaa ttcgcttttt tagcgcaatg
     9781 tcactgacct tagttgaaca ttgtttttta acggatagcg ggtttttaac accttaagcg
     9841 ccctcgacct ttatggttga gggcgttttg ctatgaacgc catcaccatt ttcccctcga
     9901 ttataaaact tgagttattc agtagtctcc cctcttgcaa ctcacaccca aaactgccta
     9961 acgaaaagtt attaattttc aatcgtattg ctatcagtat ttacattttt tcgctgtgct
    10021 agaaagggcg catttatgtt agctcgttca gggaaggtaa gcatggctac gaagaagaga
    10081 agtggagaag aaataaatga ccgacaaata ttatgcggga tgggaattaa actacgccgc
    10141 ttaactgcgg gtatctgtct gataactcaa cttgcgttcc ctatggctgc ggcagcacaa
    10201 ggtgtggtaa acgccgcaac ccaacaacca gttcctgcac aaattgccat tgcaaatgcc
    10261 aatacggtgc cctacaccct tggagcgctg gaatcggccc aaagcgttgc cgaacgtttc
    10321 ggtatttcgg tggctgagtt acgcaaactc aaccagtttc gtacgtttgc tcgaggtttt
    10381 gataatgtcc gccagggtga tgaactggat gtcccggcac aagttagtga aaaaaaatta
    10441 accccgccgc cgggtaatag cagtgacaac ctcgagcaac agatagccag tacttcacag
    10501 caaatcgggt ctctgctcgc cgaagatatg aacagcgagc aagcggcaaa tatggcgcgt
    10561 ggatgggcct cttctcaggc ttcaggcgca atgacagact ggttaagccg cttcggtacc
    10621 gcaagaatca cgctgggcgt ggatgaagat tttagcctga agaactccca gttcgatttt
    10681 ctccatccgt ggtatgaaac gcctgataat ctctttttca gtcagcatac tctccatcgt
    10741 actgacgagc gtacgcagat taacaacggc ttaggttggc gtcatttcac tcccacatgg
    10801 atgtcgggca tcaacttctt tttcgaccac gatcttagcc gttaccactc ccgcgccggc
    10861 attggcgcgg agtactggcg cgactatcta aaattaagca gtaacggcta tttgcgactg
    10921 accaactggc gcagcgcacc tgaactggac aacgattata aagcacgccc ggccaatggc
    10981 tgggatgtac gcgcagaaag ctggctaccc gcctggccgc accttggcgg taaactggtc
    11041 tatgaacagt attatggcga tgaagtggcc ctgttcgata aagacgatcg gcaaagtaat
    11101 cctcatgcca taaccgctgg acttaactat acccccttcc cgctgatgac cttcagcgcg
    11161 gagcaacgcc agggtaaaca gggcgaaaat gacacccgtt ttgccgtcga ttttacctgg
    11221 caacctggca gcgcaatgca gaaacagctt gacccgaatg aagtcgctgc acggcgtagc
    11281 cttgcaggca gccgttatga tctggtggat cgcaacaaca atatcgttct ggaatatcgc
    11341 aaaaaagaac tggttcgcct aaccctgaca gaccccgtga cagggaagtc aggagaagtg
    11401 aaatcactgg tttcgtcgct acaaaccaaa tatgccctga aaggctataa cgtcgaagcc
    11461 accgcactgg aagctgccgg tggcaaagtg gtcacaacgg gtaaagatat tctggttacc
    11521 ctgccggctt accggttcac cagtacgcca gaaaccgata acacctggcc gattgaagtc
    11581 accgccgaag atgtcaaagg caatttgtcg aatcgtgaac agagcatggt ggtcgttcag
    11641 gcacctacgc taagccagaa agattcctcg gtatcgttaa gtacccaaac attgaacgcg
    11701 gattcccatt caaccgccac actgactttt attgcgcatg atgcagcagg taatcctgtt
    11761 gtcgggctgg tgctctcgac gcgtcacgaa ggtgttcagg acatcaccct ttctgactgg
    11821 aaagataatg gtgacggaag ctatacccag atcctgacca caggagcgat gtctggcacg
    11881 ctgacgctga tgccacagct gaatggtgtg gatgcggcta aagcccccgc cgtggtgaat
    11941 atcatttctg tttcgtcatc ccgaactcac tcgtcaatta agattgataa ggaccgttat
    12001 ctctccggca atcctatcga ggtgacggta gaactgagag atgaaaatga caaacctgtt
    12061 aaggaacaaa aacagcaact gaataacgca gtcagcatcg acaacgtgaa accaggagtc
    12121 actacagact ggaaagaaac cgcagatggc gtctataagg cgacctatac cgcctatacc
    12181 aaaggcagtg gacttactgc gaagctattg gaatgaagat ttgcataccg ctggatttat
    12241 catcgacgcc aacccgcagt cagcgaaaat tgcgacatta tctgccagca ataatggtgt
    12301 actcgccaat gagaatgcag caaacaccgt ctcggtcaat gtcgctgatg aaggaagcaa
    12361 cccaatcaat gatcataccg tcacgtttgc ggtattaagc ggatcggcaa cttccttcaa
    12421 caatcaaaac accgcaaaaa cggatgttaa tggtctggcg acttttgatc tgaaaagtag
    12481 taagcaggaa gacaacacgg ttgaagtcac ccttgaaaat ggcgtgaaac aaacgttaat
    12541 cgtcagtttt gtcggcgact cgagtaccgc gcaggttgat ctgcagaagt cgaaaaatga
    12601 agtggttgct gacggcaatg acagcgccac aatgaccgcg accgtccggg atgcaaaagg
    12661 caacctgctc aatgacgtca tggtcacttt caatgttaat tcagcagagg cgaaactgag
    12721 ccaaaccgaa gtgaatagcc acgacgggat cgccacagct acgctgacca gtttgaaaaa
    12781 tggtgattat agggttacgg cctctgtgag ctctggttcc caggctaatc aacaggtgaa
    12841 ttttatcggt gatcaaagta ctgctgccct gaccctcagt gtgccttcag gtgatatcac
    12901 cgtcaccaac acagctccgc aacatatgac tgcaaccttg caggataaaa atggcaaccc
    12961 actaaaagat aaagaaatca ccttctctgt gccaaacgac gtcgcaagta agttctcgat
    13021 tagcaacgga ggaaaaggca tgacggatag taacggggtt gcaatcgcct ccctgaccgg
    13081 cacgttagcg ggcacgcata tgatcatggc tcgtctggct aacagcaatg tcagcgatgc
    13141 acagccaatg acgtttgtgg cggataaaga cagagcggtt gtcgttttgc aaacatcgaa
    13201 agcggaaatc attgggaatg gcgtggatga gacaactctg acagcaacag tgaaagatcc
    13261 gtcgaatcat ccggtggcgg ggataacggt aaacttcacc atgccacagg acgttgcggc
    13321 aaactttacc cttgaaaata acggtattgc catcactcag gccaatgggg aagcgcatgt
    13381 cacgctgaaa ggtaaaaaag cgggcacgca tacggttacc gcaacgctgg gtaataacaa
    13441 taccagtgat tcgcagccgg taacatttgt ggcggacaaa gcctcggctc aggttgtcct
    13501 gcagatatca aaagatgaga tcacaggtaa tggcgtcgat agcgcaacgc taactgcaac
    13561 ggttaaagat cagttcgaca atgaggtgaa taatcttccg gtaacattca gctcagcctc
    13621 ttcaggactc accctgaccc cgggagtaag taataccaat gagtctggca tcgcgcaggc
    13681 cactctcgca ggcgttgcct ttggtgagca gacggtcact gcatcactgg ctaataatgg
    13741 tgccagcgac aacaaaactg tgcattttat tggcgacaca gcggcggcaa aaattatcga
    13801 gttgacgcct gtcccagaca gcataatcgc cggtaccccg cagaacagct ccggcagcgc
    13861 catcaccgcc acagtcgttg ataataatgg ctttccggtg aaaggtgtga ctgtgaactt
    13921 caccagcaga acaaactctg ccgaaatgac gaatggcggc caagccgtaa cgaacgaaca
    13981 gggtaaggct accgtcactt ataccaatac ccgctcctcg atagaatcag gagcgagacc
    14041 ggataccgtt gaggccagtc tggaaaatgg tagctccacg cttagcacat caattaatgt
    14101 caacgctgat gcgtctacgg cacatctcac ctggctacag gcactttttg atacagtctc
    14161 cgcaggcgag acaaccagtc tgtatattga ggtgaaggat aattacggca acggtgtccc
    14221 ccagcatcag gtgaccctca gcgtttcacc aagtgaaggc gtgaccccca gtaataacgc
    14281 tatatatact accaaccacg acggcaattt ttacgcaagc tttaccgcta caaaagccgg
    14341 ggtttatcaa gtgacggcaa ccctcgaaaa tggcgattcg atgcaacaaa cagtgaccta
    14401 tgtgccgaac gtcacgaatg ccgaaatcac gctggcaacc tcgaaggatc cggtgattgc
    14461 cgacaataac gatctcacga cactaacagc aacagtcgct gatacagagg gcaatgcgat
    14521 agccaacact gaagtaacat ttactctgcc ggaagatgtt aaggcgaact tcacgctgag
    14581 cgatggcggt aaagcgatta ctgatgctga aggcaaagcg aaagtcacgc tgaaaggtac
    14641 aaaagcaggc gctcatactg ttacagcatc gataactggc ggtaagagtg agcagttggt
    14701 ggtgaacttt actgcggata cgctcactgc gcaggttaat cttaacgtta ccgaggacaa
    14761 ttttatcgct aataacgtcg ggatgaccag gctgcaggca acagtgactg atggaaacgg
    14821 caacccgtta gccaatgagg cggtgacatt cacgctaccg gcagatgtga gcgcaagctt
    14881 tactctcgga caaggcggtt ccgccattac tgacatcaac ggcaaggctg aagttacact
    14941 gagcggtaca aaatccggca cctaccccgt gacagttagc gtgaacaatt atggtgtcag
    15001 tgatacgaaa caggtgactt tgattgccga tgctggtacc gcaacactag cctccttaac
    15061 ctctgtatac tcattcgtcg tcagcacgac cgagggcgcg accatgactg caagcgtcac
    15121 tgacgctaac ggcaacccgg tagaaggcat aaaagttaat ttccgcggaa cctccgtcac
    15181 gctaagcagc accagcgttg aaacggatga tcggggtttc actgaaattc ttgtgacaag
    15241 caccgaagtc ggactgaaaa cagtttcagc ctctctggca gataaaccta ctgaagtcat
    15301 atcgcgatta ctgaatgcaa aagcagatat taattctgca acgattacca gtctggagat
    15361 acctgaaggt caggtcatgg tcgcacaaga cgtagcagtt aaagctcacg tcaacgacca
    15421 gtttggcaac ccgattctta atgaatctgt aacattcagt gcagagccac cagagcacat
    15481 gaccatcagc caaaatattg tctctactga tacgcatggt atagccgagg tctccatgac
    15541 gcccgaaaga aacggttcgt atatggtgaa agcatccctg gcgaatggag cctcacttga
    15601 gaaacaactg gaggctattg atgaaaaact gacactctcg gcgtccagcc cgcttatcgg
    15661 tgtcaattcc ccaacaggtg caactctgac ggcaacgcta acttctgcaa atggcactcc
    15721 agtggagggt caggtcatca actttagcgt aacgccagaa ggtgcgacgt taagtggcgg
    15781 aaaagtgaga actaactctt ctggtcaggc tccagtcgtt ctgaccagca ataaagtcgg
    15841 tacatatacg gtgactgcat cgttccataa cggcgtaaca atacagacac agacaaccgt
    15901 gaaagtcact ggcaactcaa gcaccgccca tgttactagc tttatcgctg atccatcgac
    15961 tatcgccgcc accaacagtg atttaagtac cttaaaggca acggttgagg atggcagtgg
    16021 taacctgatc gaaggtctca ctgtgtactt tgccttaaaa agcggctctg ccacattaac
    16081 gtcattaaca gcggtgaccg atcaaaacgg aatcgcgaca acaagcgtga aaggagcgat
    16141 gacaggtagc gtcacggtaa gcgcagtcac gaccgctggt ggaatgcaaa cagtagatat
    16201 aacgctggtg gcaggcccag cagacgcctc aaagtcagtc cttaagaaca atcggtcatc
    16261 attgaaagga gactttaccg atagtgctga gctacatctt gttctgcacg atatatcagg
    16321 caatccgatc aaagtttctg aagggctgga atttgtacag tcaggtacca acgtgcccta
    16381 tgtgcaagtt agtgcaattg actacagtaa aaatttctca ggcgagtaca aagccactgt
    16441 tacaggaggc ggagagggta tcacaacgct gatccctgta ttgaatggtg ttcatcaagc
    16501 tggtctgagt accacaatac aattcactcg cgcagaagac aaaataatga gcggtacagt
    16561 atcagtcaat ggtactgacc taccgacaac tacattccct tcgcaggggt tcaccggggc
    16621 gtattatcag ttgaataatg acaactttgc cccaggaaaa acggcggctg attatgagtt
    16681 ttcaagctct gcctcctggg tcgatgttga tgctaccggt aaagtgacat ttaaaaatgt
    16741 cggcagcaat tcggaaagga ttacggcgac accaaaatca ggaggcccta gctatgtata
    16801 cgaaatccgt gtgaagagtt ggtgggtgaa cgccggcgag gctttcatga tatacagcct
    16861 tgctgaaaat ttttgcagca gcaatggcta cacgctcccc agagcaaact atttaaacca
    16921 cagtagttcc cgaggcatcg ggtcactgta cagtgaatgg ggagatatgg ggcattacac
    16981 gactgaagct ggttttcaat caaatatgta ttggtcatct agtcccgcaa actcaagcga
    17041 acaatacgta gtttccctgg caacaggtga tcaaagcgta tttgaaaagc ttgggtttgc
    17101 ttatgcgaca tgttataaaa acctctgatt ttccgttata atttcttaaa gagtgctcca
    17161 ttcggagctc tcttttttac attccctcta tagagcttgc ccttctctca cagaaaatga
    17221 ataaataaaa atgcggcacc gccagaattg cgttcgatgt gactatgatc gtatccggtg
    17281 agtactgctt tgattcaaag gctggttttc tgtgatgatt gaccagcctc agagtattga
    17341 tgacaccacc gttcataaca gatcggcatt actatgcata agtactgtct catattctcg
    17401 ccaaaacact ttttaatgcc tttgcgaatt catccagact tatattgtca attttttgcc
    17461 caacgacgac tgactccgag acatcggcca ttgcgggcca tcgcgttttg taatcagcat
    17521 aaattgcaac aacaggggta ttataggcac ttgccatatg taatattgag gtgtcaggag
    17581 ttattgcgat ataagcatct tttatgattg ctgctgaacg tttaatcgag ggtggtaagg
    17641 ataaacggta gacattatta taacaatcca ctaatgccct ggctttatct tcacctttgg
    17701 gtccatgaac gatcactatt ggtatatctg tttcactttg aatttttgct attagatttt
    17761 ctgcaatcga taatgaaaat gtacgttcct gcgagctacc ttctaaattg aacgcaatgt
    17821 aagatcctaa cgatcgcatt tcctcgcgca cctcatcgag tacatcctca cttagtggca
    17881 attcatatat tggcctgact gctggaaaac ccgcctcacg catcataaat gcccatgtca
    17941 taggtacggg agcccggagt ttctgatcca tacgggacgc gttcttgcac aaaggggagt
    18001 agcaattcat ggttatacca acaacctgaa aattcgtttt tgctttcaac tgactgataa
    18061 ataacatcgt tttcaggttc tttttacgca tcccctcaat acaaagatcc ggcgtaccgt
    18121 attgctgtgt tatgttcttt gctaaatctt ttatttcttt taatgttgcg tgatcctgca
    18181 tagtcattgt gactaatgtt aatttagtct gttcaagttt aagcgcatta aagacttcta
    18241 aattaattgt cgacgttaca attaaaagat gcttaatttt atgcaattca agcgcccgaa
    18301 taacaggaaa gatggccata gcatcgccaa tctgatcggg aatatggatg acaacaaagt
    18361 ctgttttttc aatattgaaa ttataagctt tataatcgta gtaactaaat gcaatacgtc
    18421 tcaacaatga tgctaaaaac atacctaacc tcgcctccct actggttata atgcaatgca
    18481 gtctatcaga ctcatcaggg tgccattttg tgcatatgcg gacttttatg tttcatatct
    18541 ctaacctgtg ggtcctctgc ttaatcctta aacaacacca gcaactcctg cgctttcatc
    18601 ttccatcgaa tttttcatgt tgccgctaat cagccataaa atcatttgca gatgcgctct
    18661 gtcgaggtag tctcataagg ttcgtttata gatcgacggc aatgtgagtt accttttcca
    18721 tactaattat aaaaagacag tacaaacagg atcattatgg actccacgct catctccact
    18781 cgtcccgatg aagggacgct ttcgttaagt cgcgcccgac gagctgcgtt aggcagcttc
    18841 gctggtgccg tcgtcgactg gtatgatttt ttactctatg gcatcaccgc cgcactggtg
    18901 tttaatcgcg agtttttccc gcaagtaagc ccggcgatgg gaacgctcgc cgcatttgct
    18961 acctttggcg tcggattcct tttccgtccg ctcggcggtg tcattttcgg tcactttggc
    19021 gaccgactgg gacgtaagcg catgttaatg ctgaccgtct ggatgatggg catcgcgaca
    19081 gccttgattg gtattcttcc ttcattctcg accattgggt ggtgggcacc tattttgctg
    19141 gtgacactgc gtgtcattca gggatttgcc gtcggcggcg aatggggagg cgcggcgttg
    19201 ctttccgttg aaagtgcacc gaaaaataaa aaagcctttt acagtagcgg tgtacaagtt
    19261 ggctacggtg taggtttact gctttcaacc ggactggttt cattgatcag tatgatgacg
    19321 actgacgaac agtttttaag ctggggctgg cgcattcctt tcctgtttag catcgtactg
    19381 gtactgggag cattgtgggt gcgcaatggc atggaggagt ccgcggaatt tgaacaacag
    19441 caacattatc aagctgccgc gaaaaaacgc atcccggtta tcgaagcgct gttacgacat
    19501 cccggtgctt tcctgaagat tattgcgcta cgactgtgcg aattgctgac gatgtacatc
    19561 gttactgcct ttgcacttaa ttattcaacc cagaatatgg ggctaccgcg cgaacttttc
    19621 cttaatattg gtttgctggt aggtggatta agctgcctga caattccctg ttttgcctgg
    19681 cttgccgatc gttttggtcg ccgtagggtt tatatcacag gtgcgttaat cggaacgttg
    19741 agcgcatttc ctttctttat ggc
//
LOCUS       DAESHI010000076        19530 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.077, whole genome shotgun sequence.
ACCESSION   DAESHI010000076 DAESHI010000000
VERSION     DAESHI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19530
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.077"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..245
                     /locus_tag="KW011_002796"
     CDS             <1..245
                     /locus_tag="KW011_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBH7661086.1"
                     /translation="AHADYPGDEYQFGKEIAESELLWPVDRVQKSLNGELQQINGADY
                     FIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK"
     gene            complement(296..1063)
                     /locus_tag="KW011_002797"
     CDS             complement(296..1063)
                     /locus_tag="KW011_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBH7661087.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1259..2167
                     /locus_tag="KW011_002798"
     CDS             1259..2167
                     /locus_tag="KW011_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBH7661088.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2164..3426
                     /locus_tag="KW011_002799"
     CDS             2164..3426
                     /locus_tag="KW011_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HBH7661089.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDDLDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3423..4061
                     /locus_tag="KW011_002800"
     CDS             3423..4061
                     /locus_tag="KW011_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBH7661090.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            4066..4842
                     /locus_tag="KW011_002801"
     CDS             4066..4842
                     /locus_tag="KW011_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBH7661091.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            4931..6295
                     /locus_tag="KW011_002802"
     CDS             4931..6295
                     /locus_tag="KW011_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HBH7661092.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="KW011_002803"
     CDS             complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="KW011_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HBH7661093.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(7444..8583)
                     /gene="nlpD"
                     /locus_tag="KW011_002804"
     CDS             complement(7444..8583)
                     /gene="nlpD"
                     /locus_tag="KW011_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HBH7661094.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="KW011_002805"
     CDS             complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="KW011_002805"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HBH7661095.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(9343..10104)
                     /gene="surE"
                     /locus_tag="KW011_002806"
     CDS             complement(9343..10104)
                     /gene="surE"
                     /locus_tag="KW011_002806"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HBH7661096.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(10085..11134)
                     /gene="truD"
                     /locus_tag="KW011_002807"
     CDS             complement(10085..11134)
                     /gene="truD"
                     /locus_tag="KW011_002807"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HBH7661097.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="KW011_002808"
     CDS             complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="KW011_002808"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBH7661098.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="KW011_002809"
     CDS             complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="KW011_002809"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBH7661099.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="KW011_002810"
     CDS             complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="KW011_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HBH7661100.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12844..13167)
                     /locus_tag="KW011_002811"
     CDS             complement(12844..13167)
                     /locus_tag="KW011_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HBH7661101.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="KW011_002812"
     CDS             complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="KW011_002812"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HBH7661102.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="KW011_002813"
     CDS             complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="KW011_002813"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HBH7661103.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="KW011_002814"
     CDS             complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="KW011_002814"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HBH7661104.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            16411..17448
                     /gene="iap"
                     /locus_tag="KW011_002815"
     CDS             16411..17448
                     /gene="iap"
                     /locus_tag="KW011_002815"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HBH7661105.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   17469..17741
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=17591..17619
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(17838..18131)
                     /gene="cas2"
                     /locus_tag="KW011_002816"
     CDS             complement(17838..18131)
                     /gene="cas2"
                     /locus_tag="KW011_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HBH7661106.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(18128..19051)
                     /gene="cas1e"
                     /locus_tag="KW011_002817"
                     /pseudo
     CDS             complement(18128..19051)
                     /gene="cas1e"
                     /locus_tag="KW011_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
     gene            complement(19048..>19530)
                     /gene="cas6e"
                     /locus_tag="KW011_002818"
     CDS             complement(19048..>19530)
                     /gene="cas6e"
                     /locus_tag="KW011_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HBH7661107.1"
                     /translation="RFFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTIC
                     KAGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQ
                     QQLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA
                     "
BASE COUNT         4605 a         5269 c         4896 g         4760 t
ORIGIN      
        1 tcgcacatgc agattatccg ggtgatgaat atcagtttgg taaagaaata gcagaaagcg
       61 aattactctg gcctgttgat cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa
      121 acggcgctga ttattttata tttggacata tgatgtttga taacattcag acgttcgcta
      181 accagattta tattgatacc ggcacgccga aaagcgggcg gctgtcattt tataaaataa
      241 agtagtctca tgcttcttct gtgaagcatg agtaaccaaa tgttattgca ggccattatt
      301 catttttcgc taccagcaaa gagagatcct gcttcgccag cgcgcgactg gcactctccg
      361 gcaaaccgtc gtctgtgata atctgatcaa actcgcttaa tggtaacgcc agccatgtcg
      421 ccacctgacc atatttcgtc gcatcacaga ccaaaactcg ctggcggctg gcactggcaa
      481 tcgcccgttt caccgtgact ttatcttccg ctggcgtaga aatcccccgc acactccatg
      541 acgatgcaga aataaaagcc tgatcaatca tcaggctgcg cagcatggtc gcagcggctt
      601 ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc agtgtgaata attgtgcaat
      661 tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt tgtgaccaca gtcagtgact
      721 ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt tcccgcatcc agatagatac
      781 aacttcccgg ctgaacaaga cttgccgcca gcttgccaat agccgctttt tgcgtcattg
      841 ccagcgcagt ttttacctga tgagaaggtt catgcgccac gcgtcccgga gactggacgc
      901 ctccggacac cagcacaacg gctccctgct gctccagttt ttgtaaatcc cgacgaatgg
      961 tcatatgtga cacattcatt ctgtccgtta gttcagcaat actgacaatg cctttttcag
     1021 ctaccatctc aaggatgatt tggcgacgct ctacgggtat caacttttgc tccttccttt
     1081 gtcctgctga cattctacgc tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag
     1141 ttcacataaa ataacacaca atgttaattt atgtgaatca gctcaccata tcgttatctt
     1201 ccagcgctta tattcacaat atcaaacaaa atatcactta aattaacaat gagagcagat
     1261 gaaaacggga tctgagtttc atgtcggtat cgttggctta gggtcaatgg gaatgggagc
     1321 agcactgtca tgtgtccgcg caggtctttc tacctggggc gcagacctga acagcaatgc
     1381 ctgcgctacg ttgaaagagg caggtgcttg cggggtttct gataacgccg cgacgtttgc
     1441 cgaaaaactg gacgctctgc tggtgctggt ggccaatgcg gcccaggtta aacaggtgct
     1501 gtttggtgaa acaggcgttg cacaacatct gaaacccggt acggcagtaa tggtttcttc
     1561 cactatcgct agtgctgatg cgcaagaaat tgctaccgct ctggctggat tcgatctgga
     1621 aatgctggat gcgccagttt ctggtggtgc agtaaaagcc gctaacggtg aaatgactgt
     1681 catggcctcc ggtagcgata ttgcctttga acgactggcc cccgtgctgg aagccgttgc
     1741 cggaaaagtt tatcgcatag gtgcagaacc gggactaggt tcgaccgtaa aaattattca
     1801 ccagttgtta gcgggcgtac atattgctgc cggagccgaa gcgatggcac ttgcagcccg
     1861 tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc aatgccgccg gaaattcctg
     1921 gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat tacaccccgc attcagccgt
     1981 cgatattttt gttaaggatc ttggtctggt tgccgataca gccaaagccc tgcacttccc
     2041 gctgccattg gcctcaacag cattgaatat gttcaccagc gccagtaacg cgggttacgg
     2101 gaaagaagac gatagcgcag ttatcaagat tttctctggc atcactctac cgggagcgaa
     2161 atcatgatca agattggcgt tatcgccgat gattttaccg gcgcgacgga tatcgccagt
     2221 tttctggtgg aaaacggtct accaacggta caaattaacg gtgttccaac aggtaaaatg
     2281 ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa
     2341 gccacacagc aatcgctggc ggctctgagc tggttgcaac agcaaggttg caaacagatc
     2401 tatttcaaat actgctctac tttcgacagt acggcgaaag gtaatattgg cccggttacc
     2461 gatgccttaa tggatgatct cgacacgccg tttacggtct tctctccggc cctgtcggtc
     2521 aacggacgta cggtttatca ggggtatttg ttcgtaatga atcaactgct ggccgaatcc
     2581 gggatgcgcc atcacccggt aaatcccatg accgacagct atcttccccg tctggttgaa
     2641 gcgcaatcca cagggcgctg cggcgtcgtt tcggcacatg ttttcgaaca aggcgtggat
     2701 gccgttcgtc aagagctggc tcgcttacag caagagggct accgctacgc ggtgcttgat
     2761 gcgctgaccg aacaccatct ggaaattcag ggagaagcct tgcgcgatgc cccactggta
     2821 acgggcggtt ctggtctggc gattggcctg gcccggcagt gggcgcaaga aaacggtaac
     2881 caggctcgcg aagcagggcg tccgctcgct gggcgcggcg tagtgctctc cggttcatgc
     2941 tctcaaatga ccaaccgcca ggtggcacat taccgtcaaa ttgcaccagc ccgtgaagtt
     3001 gatgtggcac gctgcctctc aaccgaaact ctggccgctt atgcacacga actggcagag
     3061 tgggttctgg gccaggaaag tgtacttgct ccactggttt ttgccaccgc cagcactgac
     3121 gcattggcag caattcaaca gcaatacggt gcacaaaaag ccagtcaggc agtagaaact
     3181 ctgttttctc aactagcggc gcggttagca gcggaaggcg tgacacgctt tattgtcgca
     3241 ggcggtgaga cctccggcgt agtcacacag agcctgggca taaaagggtt tcatattggc
     3301 ccaaccattt cccccggcgt gccgtgggta aacgcactgg ataagcctgt ctcactcgcc
     3361 cttaaatctg gcaacttcgg tgatgaagcc tttttttcac gagcccaaag agagttttta
     3421 tcatgagcga tttcgcaaaa gtagagcagt ctttgcgaga ggagatgacg cggattgcca
     3481 gttcattctt tcagcgcggc tatgcaaccg gttcggctgg caatctgtcg ctgcttttac
     3541 ctgacgggaa tttactggcg acaccgacag gttcatgcct gggcaatctc gatccgcagc
     3601 ggctttccaa agtcgccgcg gatggcgaat ggttaagtgg tgacaaaccc tcgaaagagg
     3661 tgctctttca tctggcgctg tatcgcaaca atccgcgctg taaagcggtg gtgcatttgc
     3721 acagcacatg gtcgacggcg ctttcctgcc tgcaagggct ggacagcagc aacgttattc
     3781 gtccgttcac accatacgtg gtgatgcgga tgggaaatgt cccgctggtg ccttattacc
     3841 gaccgggcga taaacgcatc gcacaggatc tggcggaact ggcagcagac aatcaggctt
     3901 ttttactggc aaatcatggc ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca
     3961 atatggaaga gctggaggaa acggcaaagc tgatttttat tctcggtgac cgcccgatcc
     4021 gttatctgac cgcaggtgaa attgcggaat taaggagtta atgcaatgcc tcgttttgca
     4081 gctaatttat ccatgatgtt caccgaagtg ccttttattg aacgcttcgc cgcagcgcga
     4141 aaagccgggt tcgatgctgt ggaatttctg tttccctatg actactccac cctgcaaatc
     4201 caaaagcaac tggagcaaaa ccatctgaca ctggcgctgt tcaataccgc tcccggagat
     4261 attaatgcgg gggaatgggg attatccgcc cttcccggac gtgagcacga agcacacgca
     4321 gacatcgacc tggcactgga atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg
     4381 gcaggcgtcg tgcccgctgg cgaagacgct gaacggtacc gggcagtatt tatcgataat
     4441 ctccgctacg ctgccgaccg ctttgcgcta cacggcaagc gaatattagt tgaagcactc
     4501 agccccgacg ttaagcctca ttatctcttc tccagccagt atcaggcact ggctattgtt
     4561 gaagaggttg cgcgagataa cgtgtttatt caactagata cttttcatgc gcaaaaagta
     4621 gatggcaacc tgacgcattt aatccgcgac tacgctggga aatatgcgca cgtacaaatt
     4681 gccggactac ctgatcgaca tgaaccggac gatggagaaa tcaactatcc gtggctgttc
     4741 cgcctgttcg atgaggtggg atatcagggc tggatcggtt gtgaatataa acctcgtggc
     4801 ctcaccgaag aagggcttgg ctggtttgac gcctggcgct aattcgttaa atccctaatt
     4861 acaacgtacc catacatccc cccatctacg gatggggtag gttaactata actttcagac
     4921 agggtttccc atgtccacaa ttacattgtt atgcattgcg ttagctggcg taatcatgct
     4981 gttgctgctg gtcatcaagg caaaggtaca accattcgtt gctctgctcc tcgtcagcct
     5041 gttagtcgca cttgcggcgg gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg
     5101 gatgggcggt gtgcttggtt ccgtcactat tattattggt ctgggcgcta tgctggggcg
     5161 tatgatcgaa cactctggtg gtgcagagtc actggctaat tatttcagtc gcaagttagg
     5221 tgacaaacga actatcgctg cgctgactct ggcagcgttc ttcctcggta ttcccgtctt
     5281 ctttgatgtc ggctttatta ttcttgcgcc aatcatttac ggttttgcca aggttgccaa
     5341 aatatcgcca ctcaaatttg gcctgcctgt cgctgggatc atgctaactg tacatgtggc
     5401 ggtaccgccg catccaggcc ctgtcgccgc agcggggtta ctccacgcag acatcggctg
     5461 gctaaccatc atcggtattg cgatttctat tcccgtaggg attgttggct actttgcagc
     5521 gaaaataatc aataagcacc aatatgcgat gtcggtggaa gtactggaac agatgcaact
     5581 ggctccggcc agtgaggaag gcgcgacaaa attaagcgat aaaataaatc caccgggcgt
     5641 cgcgctggtc acctcgctaa ttgttattcc tatcgcgatt atcatggcgg gtacggtttc
     5701 cgcaacactg atgccgcctt cgcatcccct gcttggtacg ctacagctga tcggctcacc
     5761 aatggtagct ctaatgattg cgctggtgct ggcattctgg ttattggctt tacgtcgcgg
     5821 ctggagctta caacacacca gcgacattat gggctcagcc cttcccactg cggcggtagt
     5881 gattttggtt actggtgctg gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg
     5941 caaagccctt gccaacatgc tacaaatgat tgacctgcct ctgttaccag ccgcatttat
     6001 tatttcactg gcgctgcgtg catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg
     6061 cgggttactc tcagaagcgg tgatgggatt gaatccgatt cagtgcgtat tggtgacgct
     6121 ggcagcctgc tttggtgggc ttggggcgtc acatattaat gactcagggt tctggattgt
     6181 gaccaaatat ctggggttgt cggtagcaga cggtctgaaa acctggacag tgttaacgac
     6241 cattctcggt tttaccggat tcttaattac ctggtgcgta tgggcggtaa tttgacctta
     6301 ataaaaaggt ccgatgggca tcggaccttt tattgtgcac agaaaaggcc agcctcgctt
     6361 gagactggcc tttctgacag atgcttactt actcgcggaa cagcgcttcg atattcagcc
     6421 cctgcgtttg caggatttca cgcaaacggc gcaggccttc aacctgaatc tggcgaacac
     6481 gttcacgggt gaggccaatt tcacgaccta catcttccag tgttgccgct tcgtacccca
     6541 gcaaaccgaa tcgacgtgcc agtacttcac gctgtttggc gttcagctcg aacagccatt
     6601 tgacgatgct ctgcttcata tcgtcatctt gcgtggtatc ttccggaccg ttctcttttt
     6661 catcggccag gatgtccagc aacgcttttt cggaatcacc acccagcggg gtgtctaccg
     6721 aggtaatgcg ctcgttaaga cgaagcatac ggctgacgtc atcaactggc ttatccagtt
     6781 gctctgcgat ctcttccgca cttggttcat ggtccagctt atgggacaac tcacgtgcgg
     6841 ttcgcaggta aacgttcagc tcctttacga tgtgaatcgg caaacgaata gtacgggttt
     6901 ggttcataat cgcccgttca atcgtctggc gaatccacca ggttgcgtat gttgagaagc
     6961 ggaaaccacg ttccgggtca aacttctcta ccgcgcggat cagccccagg ttgccctctt
     7021 cgataaggtc cagcaacgcc agaccacgat tgccataacg gcgggcaatt tttaccacca
     7081 gacgcaagtt actctcgatc atccggcggc gagaggcgac atctccacgc agtgcgcgac
     7141 gcgcaaaata aacttcttct tcggccgtta acagtggtga ataaccaatc tcaccaaggt
     7201 aaagctgagt cgcgtccaac acacgctgtg tggctccctg cgataacagt tcctcttcgg
     7261 ccaaatcgtt atcactgggt tcctcttcta ctaaggcctt ttcgtcaaaa acctcaactc
     7321 cgttctcatc aaattccgca tcttcattta aatcatgaac tttcagcgta ttctgactca
     7381 taaggtggct cctacccgtg atcccttgac ggaacattca agcaaaagcc tggttccgcc
     7441 gatttatcgc tgcggcaaat aacgcagcgg gtttacggat ttccccttgt aacgaatttc
     7501 aaaatgcaag cgtgttgaac tggttccggt gctacccatg gttgctattt tttgccccgc
     7561 cttcacttct tgttgttccc ggaccagcat tgtgtcgtta tgggcgtagg cactcaggta
     7621 atcatcatta tgtttgatga taatcagatt accgtagccg cgcagcgcgt taccggcata
     7681 aacaacgcgg ccatctgcgg tcgcgataat tgcctgtcct ttgctgcctg cgatatcaat
     7741 ccccttgttg cccccctcag aagcgccaaa ggtttcgatc actttgccct cagtcggcca
     7801 gcgccaggtg gagataggcg tactggttga tgtactgctg acagtcggct cggttgtgct
     7861 tgctgttggt accgttacag gcgctgtgac tgtggtcgca gttggcttgt tgttcggcaa
     7921 cattttgtta gcactctgtt cacccgaaga ctcagaatac gtaattgtcg gttgcgacgc
     7981 aacagcaacg gtggaatttt gtgcaggctt gatcacaact ccttgctctg ctgcgtcggc
     8041 ctgggtaatg gcatttccgc cagtgattgg cgtaccggaa gcattaccca cctgcaaggt
     8101 ctgaccaacg ttcagcgcgt atggtgcctg aatattgttg cgctgagcaa ggtcacggaa
     8161 atcgttgcca gtaatccagg cgatatagaa aagtgtgtcg ccttttttca cggtataggt
     8221 actgccgcta taactgcctt tcggaatgtt cccatactga cggttataga cgatgcgtcc
     8281 gttttccatc tgtaccggct gctgagctac tggctgcact ggctggattt gcggttgttg
     8341 agtagcctga atttgtggct gctgtaccgg ctgaatttgc ggttgctggg ctgtagacgt
     8401 cgtccccatt ttcggcggcg gcgtaatcaa cataccagaa ttagtatttg caggcgcatt
     8461 gccattaacg gagctgaccg gtgccggtgg atttgaagtg tcagaacagc ctgccagcca
     8521 tagcgaaacc agtgacaaag cagcaatgcg gcgaacggtg aattttgggc ttcccgcgct
     8581 catttatccc ccaggaaaaa ttggttaata accagtgaca taattaccgt gcaaggcacc
     8641 ctactgaaca ctggaaaaga tgttcacgat acgctgacct gcggcaaaat aaccaggaaa
     8701 aatccaggta tttcctcacg ttttaagcca gctcaccctt cactaaaggg acaaagcgca
     8761 cggcctccac ggtatcgata ataaattcgc ctccccgacg acgcacccgt ttcaaatact
     8821 ggtgctcctc ccctacgggt aagacgagaa tcccgccttc gtccagctgc gtcattagcg
     8881 cagttggaat ttccggcggt gccgccgtaa caatgatagc gtcaaacggc gcacgtgcct
     8941 gccaaccttg ccatccatcg ccatgacggg ttgaaacatt atgtaaatca agatttttca
     9001 ggcggcgacg tgcctgccac tgcaagcctt taatccgttc aaccgagcaa acatgctgga
     9061 caagatgcgc taggattgcc gtttgatatc ccgaaccagt gccaatttcc agcacccgcg
     9121 actgcggcgt cagctcgagt aattcggtca ttcgcgccac catatacggc tgcgaaattg
     9181 tctgcccctg acctatcggc aaggcgatat tgtcccaggc tttttgttca aacgcttcat
     9241 caacgaattt ttcacgcggc acggcggcaa gtgcattcag cacctgctca tcctgaatac
     9301 cttgcgcacg taattgatcc agaagtgctt gtacgcgtct gcttaccatt gcgtgccaac
     9361 tcccacgctg tttaaccagt ctgaaaccac atcttgcgcg ctatgcgcag ttaaatccac
     9421 atgcagcggc gtgatggaga catagccctc atctaccgca gcaaaatcgg tccccggacc
     9481 ggcatcacat ttaccgcccg gcgggccaat ccagtacagc gtattgccgc gcggatcttg
     9541 ctgcgggatc acctgatctg ccggatgtcg tgtaccgcag cgcgtcacgc gaataccttt
     9601 gatttgatcc aagggtaaat ccggaacgtt aatattaaga atacgtccgg tgcgcagcgg
     9661 ctctttacac agtgcgcgca aaattgaaca ggttaccgcc gcggcagtgt cgtaatgttt
     9721 atgcccgtca agcgagacgg ccagcgccgg aaaacctaaa tgacggcctt ccatcgcggc
     9781 ggctaccgta ccggaataaa taacgtcatc ccccagattc ggcccggcgt taatgccgga
     9841 cacaacaatg tccgggcgcg gacgcatcag agcattcacg ccaagataga cgcaatcggt
     9901 cggggttccc atttgcacag caatatcacc attttcaaag gtaaacgtgc gcagggagga
     9961 ttccagtgtc agagaatttg aagcgccgct gcggttacga tcgggggcga ccacctgaac
    10021 gtcagcaaac tcacgcaagg ctttcgccag cgtttgtata ccgggtgcat gtaccccgtc
    10081 atcattactc agcaatatgc gcataatcac ctgttgtgtt gataagttcc ctgacaacgc
    10141 tggttgcaaa actacccgcc ggaagccaga aacggatctc tacggtgacg tcatcccacc
    10201 aattccagct taattgttgc ggatacagca acatcgctct gcgcgcggct tcaacttttt
    10261 cgcgcaccag taaagcttgt aattcagttt ctgcggcgac agctgccttt tcgaatgcca
    10321 gcgcttcacg ctgagttccc cattcgccac tacccggcaa tgcggcggtt atcatcagct
    10381 ctttatcgtt gacgcgacgc tgtaattccg ccagttcttc ggtggttgcg acaaaccagc
    10441 taccacgtcc ggctaattgt agcgcatcgc cgtcaacaac ttgattaacg tctgcttttt
    10501 tgaggcgctc agcaacaatc tgattaaaca acgcactgcg ggctgccgac aaccaaaaac
    10561 tccgtttatt gcgatcgcgc accggagtat tggtttgcgc ccagcgctgc gccccctgca
    10621 agttgctacc gccaatccca aaacgttggg caccgaagta gttcggtaca ccttttacgc
    10681 aaatatcgat cagacgttgt tcaacgtcat cgcgattgct cacttcgcgc agaaccaggg
    10741 taaaggcgtt acctttcagc gcgcctaaac gcagcttgcg cttgtgccgc gcatactcca
    10801 gcacctggca gccttccagt tgaaaggcgc tcagatcggg catttccttg cctggcacgc
    10861 gagcgcataa ccactgttcc gtaacagcat gtttgtcttt ttgcccagcg aagctgactt
    10921 cacgggcatg aattttcagg aatttcgcca gtgcatccgc cacaaaacgg gtattgcagc
    10981 cgtttttgag gattctaacc agaatatgct caccttcacc atcaggctca aagcccaaat
    11041 cttccaccac cacaaagtct tccggattgg ctttcagcag cccggtgcct tgcggtttac
    11101 cgtggaggta agtgagatta tcaaactcaa tcattttgtt gccttaatga gtagcgccac
    11161 cgcttcacag gcaatccctt ccccacgtcc ggtaaatcca agtttttccg tagtagtggc
    11221 tttcacgtta acatcatcca tatggcagcc gagatcttcg gcaataaaca cgcgcatttg
    11281 tggaatgtgc ggcaacatct tcggtgcctg agcgataata gtgacatcga cgttgcccag
    11341 ggtataaccc ttcgcctgaa tacgacgcca ggcttcgcgt agcagctcgc ggctgtcggc
    11401 acctttaaat gccggatcgg tatccgggaa cagcttgccg atatccccca gcgccgccgc
    11461 gccaagcaat gcatcggtca acgcatggag cgccacgtcg ccatcagaat gcgccagcaa
    11521 tcctttttcg taaggaatgc gtacgccacc aatgataatt gggccttcac cgccaaaggc
    11581 atgtacgtca aaaccgtgtc caattcgcat tatgtattct cctgatggat ggttcgggtg
    11641 aggtaaaact cggccagtgc caaatcttcc gggcgcgtga ctttaatgtt atccgcacgg
    11701 ccttcgacca actgaggatg gaatccgcaa tattccagcg ccgaggcttc gtcggtaata
    11761 gtcgcgcctt catttagagc gcgcgtcaga cagtcatgta acagctcacg agggaaaaat
    11821 tgcggcgtca gcgcgtgcca taagccgttg cgatcaacgg tatgagcaat ggcatttttg
    11881 cccggttcgg cacgtttcat cgtatcgcgc actggtgcgg ctaggatccc tcccgtgcgg
    11941 ctggtttcgc tcaacgccaa caatcgcgcg aggtcatcct gatgcagaca aggacgagcg
    12001 gcgtcatgca ccaataccca ctgcgcgtcg ccagcggctt tcaaacctgc cagcacggaa
    12061 tcggcacgct catcaccgcc atctacaacg gtgatttgcg gatgattcgc cagaggaagt
    12121 tgtgcaaaac ggctatcgcc aggacttatg gcaatgacga cacgtttcac ccggggatgc
    12181 gccagcagcg catgcaccga gtgttcaaga atggtttgat taccgattga gagatattgc
    12241 ttaggacatt ccgtttgcat tcgacggcca aatccggccg ccggaaccac ggcgcaaaca
    12301 tccaaatgag tggttgccat gttaattccc gggctgattt atcgattgtt ttgccccgca
    12361 gactgtgcgc gcttcgacgc gtcaggcacc agacgataaa aagtttcgcc cggcctggtc
    12421 atgctgagtt cattacgcgc acgctcttcg agcgcctcct ggccgccatt gagatcgtca
    12481 atttcggcaa aaagttgatc gtttcgcgct ttaagtttcg cgtttgtagc ttgctgtgcc
    12541 gccacatcat cattgacgcg ggtatagtca tgtataccgt tcttaccgaa ccacagcgaa
    12601 tactgtagcc agaccagaat agccagcaac agcagcgtta gtttacccat cctgccccct
    12661 gaaaaacggc atcatcatcc catgcatccg aagacgactc tacatcctct gttggggata
    12721 ccgcgacaac gcgggcaaat gtaccacatt tgtccattgt tacgtatacc cagggcgtgc
    12781 agaacataat ctcattatta gttacggttt gaattatgaa cagaggagac aagaaagtac
    12841 aaattagccc agtagccaca taaacagtgc gccaaacata atgcctactg tcaccagggt
    12901 gaaaacaata ctgtagcgta gctttccgtc catcaatgaa tgcagcgcaa tccccaccac
    12961 taccgcgacg ggcatcagcg ccagaaagaa aggccaggtg tagataaaga agaacagcgt
    13021 gttagagcca taaatcaaca tcggcatcgc cagcgcaaat aaccaggaga taaaaccgac
    13081 cacggcacca ggcagtgacc atgtggtttc ttcatcctca gtaaggctgt cgttatttgt
    13141 tagtgtaatg ttatggctat tacgcatatt tgatcctgtt actttgacga accgggcatg
    13201 gaaacccggt ggtgtctcag gatctgataa tatcgttctg tctcaacaga tctaataatt
    13261 gctgtaccaa atttgttact aattgttcac cattgagatg aatttctgcc gattcaggcg
    13321 cttcgtaaac ggaatctatt cccgtaaagt tgcgcagttc accggcacgc gctttcttat
    13381 ataagccttt tggatcgcgg gcttcgcaaa tcgccagcgg cgtatcgaca aacacttcga
    13441 taaagcgccc ttctcctacg cgttcacgaa ccatctggcg ttcggcgcgg tgtggcgaga
    13501 taaatgcggt cagcaccacc agtccggctt caaccatcaa attcgccact tcaccgacgc
    13561 gacggatatt ctctttacga tcggcatcgc taaaaccgag atcgctgcat aatccgtggc
    13621 gaacattatc gccatccagc agatacgtac tgacgccgag tttatgtaac gcctcctcca
    13681 gcgccccggc gaccgttgat ttaccggacc cggagaggcc ggtaaaccac agcactacac
    13741 cacgatgacc gtggtgtagc tcgcgttgtt gcacagtgac cggatggcta tgccagacga
    13801 cgttttcgtc atgcagcgcc attatttatc ccccagcaaa tcgcgcgcgc cccagtgcgg
    13861 aaagtggcga cgaaccagag cattcaattc cagttcgaat gcactgaatt cagatggcgc
    13921 agcagttgcc tggctaactg gctcgtgcac cataccggca cccacggtca cattgctcag
    13981 gcgatcgata aaaatcagcc cacccgtcac cggattttgt tgataacgat ctaacaccag
    14041 cggctcgtca aaagtgagat ccacgaggcc gatcccattc agtggcaggt tttcaacttc
    14101 acgctgggta aggttattaa tatcaacctg atagcgaatg ccatcaacac gcgcgcgcgt
    14161 cttcttaccg gcaattttga tgtcgtaact ctgccctgga gaaagcggct gttccgccat
    14221 ccataccaca tccaccgacg cgctctgcac cgccggtaac gcttcgtctg ccgccagcag
    14281 cagatcgcca cggctgatgt cgatctcatc cgtcagcacc agggtgatcg cttctccggc
    14341 aaaggcttct tcgcgatcac catcaaaagt cacgatccgc gcgacgtttg attccacacc
    14401 agagggcagc acttttacac gttgcccgac ttccacgcga ccggatgcca gcgttccggc
    14461 gtagccacga aaatcgagat tcgggcgatt aacgtactgc accgggaagc gcattggctg
    14521 agcatccacc actcgctgga tctccacggt ttccagcact tcgagcagtg tcggaccgct
    14581 gtaccacggc atactttcac tttgcgatgc cacgttgtcg ccttccagtg cagagagcgg
    14641 cacaaagcgg atatccagat tacccggcag ctgcccggca aaggtcagat aatcttcacg
    14701 aatacgggtg aacgtctctt cactgtaatc caccagatcc attttgttga tcgccacgac
    14761 cagatgtttg atccccaaca gtgtggagat aaaactgtga cgacgggttt gatcgagcac
    14821 gcctttacgg gcatcgatca gtaagatcgc cagttcacat gtcgatgcgc cagtcgccat
    14881 attgcgagtg tactgctcgt gccctggggt gtcggcgata ataaatttac gcttctcggt
    14941 agagaaatag cggtaggcca catcaatagt gatgccctgt tcgcgctcag cttgcaggcc
    15001 gtccaccagc agagccagat ccagcttttc gccctgggta ccgtgacgct tactgtcgtt
    15061 atgcagcgat gagagctgat cttcatagat ttggcgagta tcgtgcagca ggcgaccaat
    15121 cagagtgctt ttgccgtcat cgacgctgcc acaggtcaga aaacgcagca ggcttttatg
    15181 ttgttgcgca atcatccagg cttcgacgcc gccttcattg gcgatttgtt gtgcaagtgc
    15241 ggtgttcatc ttaaaaatac ccctgacgtt ttttcagctc catagatccc gcctggtcgc
    15301 ggtcaatcac gcggccctga cgttcactgg tggtggaaac cagcatctct tcgatgattt
    15361 ccggcagtgt ttgtgcattt gactccaccg caccggtcag cggccagcag cccagcgtac
    15421 ggaaacgcac catccgtttt ttaatcactt cgcccggttg caggtcgata cggttgtcat
    15481 caatcatcat caacataccg tcgcgttcca gaaccggacg ttccgcagcg agatacagcg
    15541 gcacaatgtc gatattttcc agccagatgt attgccagat atcctgctcg gtccagttag
    15601 agagtgggaa gacgcggatg ctttcgcctt tgttaatttg cccgttgtag ttgtgccaca
    15661 gctccgggcg ctgatttttc ggatcccagc gatggaagcg gtcacggaaa gagtagattc
    15721 gctctttagc gcgggatttc tcttcgtcac ggcgcgcacc accgaaggcg gcatcaaaac
    15781 cgtatttgtt cagcgcctgt ttcaggcctt cagttttcat aatatcggta tgtttcgcgc
    15841 tgccgtgcac gaatggatta atccccatcg ccacgccttc cgggttttta tgcaccagca
    15901 gctcgcagcc gtaggctttc gccgtacgat cgcggaactc atacatctcg cggaatttcc
    15961 agccggtatc gacatgcagc aacgggaaag gcagcgtacc tggataaaac gccttgcgcg
    16021 ccaaatgcag catgacgctg gaatctttac cgatagagta gagcatcacc ggatttgaga
    16081 attctgccgc cacctcgcga ataatgtgga tgctttccgc ctccagttgc cgcaggtgag
    16141 taagtcgtat ttgatccata accgttcctt tgcaataccg ctattttctt gccatcagat
    16201 gtttcgacta tagggagcgt aagagaacga atgaaattac caattagaat gagtagttcc
    16261 ttaacggaat aacgatttgg caaagctaat atcaaaaagt gcttaaggca ccggatttct
    16321 ggcgtttagg aagatttgaa attgttttag cgcagcggca gtttcatact atggcggtaa
    16381 aaaaatttgc atggtattta aggactcact atgttttccg cattgcgcca ccgtaccgct
    16441 gccctggcgc tcggcgtatg ctttattctc cccgtacacg cctcgtcacc taaacctggc
    16501 gattttgcta atactcaggc acgacatatt gctactttct ttccgggacg catgaccgga
    16561 actcctgcag aaatgttatc tgccgattat attcgccaac agtttcagca aatgggttat
    16621 cgcagtgata ttcggacatt taatagtcgg tatatttata ctgcccgcga taatcgtaag
    16681 agctggcata acgtgacggg aagtacggtg attgccgctc atgaaggcaa agcgccgcag
    16741 cagatcatca ttatggcgca tctggatact tacgccccgc tgagcgatgc tgacgccgat
    16801 gccaatctcg gcgggctgac gttacaagga atggatgata acgccgcagg tttaggtgtc
    16861 atgctggaac tggcagaacg cctgaaaaat acgcctaccg agtatggtat tcgatttgtg
    16921 gcgaccagcg gcgaagagga aggaaaatta ggcgctgaga atttactcaa gcggatgagt
    16981 gacaccgaaa agaaaaatac gctgctggtg attaatctcg ataacttaat tgttggcgat
    17041 aaattgtatt tcaacagcgg tgtaaaaacc cctgaagcag taaggaaatt aacgcgcgac
    17101 agggcgctgg caattgcgcg cagtcacgga atagccgcaa cgaccaatcc gggtttgaat
    17161 aaaaattatc cgaaaggcac tgggtgttgt aatgacgcag aaatattcga caaagcgggc
    17221 attgctgtac tttcggtgga agcgactaac tggaatcttg ggaataagga tggttatcag
    17281 caacgcgcaa aaacagccgc attccctgcg ggaaatagct ggcatgacgt aagactggat
    17341 aatcaacagc atattgataa ggcacttcct ggaagaatag aacgtcgctg ccgtgacgtt
    17401 atgcggataa tgctacctct ggtgaaggag ttggcgaagg cgtcttgatg ggtttgaaaa
    17461 tgggagctgg gtgttctacc gcaggggcgg ggaattctaa gtgatatcca tcatcgcatc
    17521 cagtgcgccc ggtttatccc cgctggcgcg gggaactctt actgcttggt atgcggaatc
    17581 acaccctgaa cggtttatcc ccgctggcgc ggggaacacc ctgacgcgcc gcagtattta
    17641 tctgctctgg ccggtttatc cccgctggcg cggggaacac gggtgcgtgt ggctgccagt
    17701 gccggagaac ggcggtttat ccccgctggc gcggggaaca cactaagcat acataactgt
    17761 ttttaaacaa atttattccg catcaacaat ctaccaacta aattaaaaca tttccttatt
    17821 tttaaagaac ggataactta ttgattatca acaggaagaa aagaaaccaa acgtaaccca
    17881 tccaaatcca ccggaatacg tctgttttct ccccaggtct gaaattcaaa acccgactcg
    17941 atattggtcg cccaggccat caccacattt ccgcaaccag ccagttgggt aatttgctgc
    18001 cagatcatct cccgaatacg ttttgatgta tcaccaacat acacaccggc acgcacttcc
    18061 agtagccaga ttgcgagccg tccacgtaag cgcggaggga cattttctgt aacaaccacg
    18121 accatgctca tccgccgcgc ccccggtgac cactatcacc cagcgtttca ggttcaggga
    18181 tggcaggcgg taacatatcc ggcgcgggtt gtggtggttc aatttcacct gcagcaagga
    18241 cttcctcaat taacggtatt aatttgcccg ttaacttagt gctacggaaa atatcgcgac
    18301 aggctaatct gacttcttta tcaggttctg cgggttgcct cgctgctatt tcaaatgcct
    18361 ttggcacaac cgaatcaaat ttaatgatat cggctatgtc ataaacaaat gaaagcggtt
    18421 tgccactatg gataaaccca atagcgggcg catatcccgc ggctaatact gccgcttcag
    18481 atataccgta cagacatgat gtagcagcac tgatgcagcg attcacaaca tcgccttttt
    18541 cccagtcttt aggatcgtat ttgcgaccat tccatttcac accatattgt ttcgccagta
    18601 atgcatacgt ctggcgaacg cgagatccct caattccacg taactgctcc actgaacggc
    18661 gagctggcgg tggttcacga aaacgtaatt catacatttt gcgcaccacc ttcaaccgta
    18721 gatcttccgt taaagccagc tttgcctagt agagtaattt atctgcccgc gcccctccgg
    18781 gttgtccgga ggagtaaacg cgaacgcccg cttcaccgac ccagaccagc agtgttccca
    18841 ccgtggcggc cagatggact gccgcgtggg aaactcttgt tcccggttcg agcataatgc
    18901 aggcgaccga tcccactgga atatgcgtgc ggatcccggt tttgtcgatc agcacgaaag
    18961 cgccgtccag tacgtcgatt tgaccgtact ggaggaagat catggaagtg cgatctttta
    19021 atgggatcgg gctcagcggt acaaacgtca cgcctctgct ccgggtttga tcagcatcag
    19081 accacaaccg aacgcccggc ttttgccata cccctgactg agacgttgca gaaataaccc
    19141 aggagcggtg accgtaagca tccccgtata atccacgcta ctgaactgga tcagttgccg
    19201 ggagttttcc cgccgcagtt gctgttgtcg gtaggcatca accgaagtat ccagcagtgt
    19261 aaaaccgctt ctctccccct gcgctgccag ccagtccagc gcagcctgtt gttgatgcaa
    19321 ccagacatca cttccttcca cctgccccct cacctgccgt ttcgcctcca tcagcaagtc
    19381 gtggcgtttg cccgctttac aaattgttgg attcgcccgc aggttgaaac aaagggattg
    19441 tccggtacgc aactctggcg caaatggtcg gcattcgatg gtgaaaatat cactttccgc
    19501 cgggcgttcc tgcgacagga caaaaaagcg
//
LOCUS       DAESHI010000077        19481 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.078, whole genome shotgun sequence.
ACCESSION   DAESHI010000077 DAESHI010000000
VERSION     DAESHI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19481
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.078"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            481..930
                     /gene="alaE"
                     /locus_tag="KW011_002819"
     CDS             481..930
                     /gene="alaE"
                     /locus_tag="KW011_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HBH7661108.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
     gene            complement(967..1311)
                     /locus_tag="KW011_002820"
     CDS             complement(967..1311)
                     /locus_tag="KW011_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HBH7661109.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1463..1792
                     /locus_tag="KW011_002821"
     CDS             1463..1792
                     /locus_tag="KW011_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBH7661110.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            2040..2285
                     /gene="nrdH"
                     /locus_tag="KW011_002822"
     CDS             2040..2285
                     /gene="nrdH"
                     /locus_tag="KW011_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBH7661111.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            2282..2692
                     /gene="nrdI"
                     /locus_tag="KW011_002823"
                     /pseudo
     CDS             2282..2692
                     /gene="nrdI"
                     /locus_tag="KW011_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            2665..4809
                     /gene="nrdE"
                     /locus_tag="KW011_002824"
     CDS             2665..4809
                     /gene="nrdE"
                     /locus_tag="KW011_002824"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBH7661112.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4819..5778
                     /gene="nrdF"
                     /locus_tag="KW011_002825"
     CDS             4819..5778
                     /gene="nrdF"
                     /locus_tag="KW011_002825"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBH7661113.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            6134..7336
                     /gene="proV"
                     /locus_tag="KW011_002826"
     CDS             6134..7336
                     /gene="proV"
                     /locus_tag="KW011_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HBH7661114.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            7329..8393
                     /gene="proW"
                     /locus_tag="KW011_002827"
     CDS             7329..8393
                     /gene="proW"
                     /locus_tag="KW011_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HBH7661115.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8451..9443
                     /gene="proX"
                     /locus_tag="KW011_002828"
     CDS             8451..9443
                     /gene="proX"
                     /locus_tag="KW011_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HBH7661116.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9734..10918
                     /locus_tag="KW011_002829"
     CDS             9734..10918
                     /locus_tag="KW011_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH7661117.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            11042..11779
                     /gene="ygaZ"
                     /locus_tag="KW011_002830"
     CDS             11042..11779
                     /gene="ygaZ"
                     /locus_tag="KW011_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HBH7661118.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            11769..12104
                     /gene="ygaH"
                     /locus_tag="KW011_002831"
     CDS             11769..12104
                     /gene="ygaH"
                     /locus_tag="KW011_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HBH7661119.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            12195..12725
                     /gene="emrR"
                     /locus_tag="KW011_002832"
     CDS             12195..12725
                     /gene="emrR"
                     /locus_tag="KW011_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HBH7661120.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            12852..14024
                     /gene="emrA"
                     /locus_tag="KW011_002833"
     CDS             12852..14024
                     /gene="emrA"
                     /locus_tag="KW011_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HBH7661121.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYCFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            14041..15579
                     /gene="emrB"
                     /locus_tag="KW011_002834"
     CDS             14041..15579
                     /gene="emrB"
                     /locus_tag="KW011_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HBH7661122.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(15643..16158)
                     /gene="luxS"
                     /locus_tag="KW011_002835"
     CDS             complement(15643..16158)
                     /gene="luxS"
                     /locus_tag="KW011_002835"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBH7661123.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(16308..17864)
                     /gene="gshA"
                     /locus_tag="KW011_002836"
     CDS             complement(16308..17864)
                     /gene="gshA"
                     /locus_tag="KW011_002836"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBH7661124.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            complement(17937..18365)
                     /locus_tag="KW011_002837"
     CDS             complement(17937..18365)
                     /locus_tag="KW011_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBH7661125.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(18362..18928)
                     /gene="yqaB"
                     /locus_tag="KW011_002838"
     CDS             complement(18362..18928)
                     /gene="yqaB"
                     /locus_tag="KW011_002838"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HBH7661126.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(19209..19285)
                     /locus_tag="KW011_002839"
     tRNA            complement(19209..19285)
                     /locus_tag="KW011_002839"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19249..19251),aa:Arg,seq:acg)
BASE COUNT         4637 a         5040 c         4915 g         4889 t
ORIGIN      
        1 gcgagacgac gctgcgacgt tctgttcgcc atgatgcgcg acgggacttt ttataccccg
       61 caggggtcat aacatgcttg acaacttaat aggggcaccc ccctcactgg tgctttctgc
      121 tgttaatgta cattttctgg catcaaaaat gctgtaaatg acacggtcac gacatacacc
      181 gaattctcta cgcttatagc gttacctcac ccccaaactt tcattagtga aattaaaggc
      241 aaggtcatat ctaattgatt taattgaaaa taaatcatat cggcatgtaa aaatccacaa
      301 tgtacaaaaa acgagccgtt acggaatatt ttatctacaa aaactgacta aataaaaatt
      361 tttcactaat tgaatagtca tagccagcga tatacgctat gcgaaaatgc agatggcaat
      421 gagatccact gctttcatct ccattaacat cccattacgc ttttattaag gagcattagc
      481 atgttctcac cgcagtcacg cttgcgtcat gcagttgcag atacgttcgc gatggttgtt
      541 tactgttctg tcgtgaacat gtgtattgaa gttttcctct ccggaatgag cttcgaacag
      601 tctttttatt ccagattggt agcgattccg gtgaacatct taattgcatg gccatacggt
      661 atgtaccgcg atctatttat gcgcgcggca cgcaaagtta gcccgtcggg ctggataaaa
      721 aatctggctg atatcctggc ttatgtgacg ttccagtcac cggtgtatgt ggcgatcttg
      781 ttagtggtgg gcgcagactg gcatcagatt atggcggcgg tcagttcaaa catcgttgtt
      841 tcgatgttga tgggggcggt ttatggctac ttcctcgatt attgccgccg actgtttaaa
      901 gtcagccatt accagcaggt aaaagcctga ctcttctttt cgcgactggc atcgccagtc
      961 gcgtctttaa ctggcttcgc caaataaacc attcaaataa cgttcaagcg caacacgcga
     1021 actgaagcca tgcgggatac cgtaatgctc gtgtcgctcg cctgctaaat agagcggaaa
     1081 ttgttgataa tgatcgcaag ttttaatatc ggaagcgggt tcagccagtg tcgaactacg
     1141 ttcttttaat gttggatgaa ttaccagtgc ggtacgtccc attcgcgctt cgcgattaac
     1201 atagacataa ttttccccac ggcggtaacc ataagctttc tgggttacca catcgacagt
     1261 aaaaccgact ttttcaagta cgcgcgccac ctcgtctggt cgtaaataca tatttgatcc
     1321 tcgttattat caaccgcggg cctaccttac ctgattgcgc attcacaacg ctttcagaaa
     1381 agtccataaa gccgcgatga acctcagtta agaaaattat ggtctacact gaaaattaca
     1441 tcgaattcta atgggggatc atatgtttaa ccgaccgaac cgcaacgacg ttgatgatgg
     1501 cgtgcaggat atccagaatg atgtcaatca attagctgac agcctcgaat ctgtattgaa
     1561 gtcctggggc agcgacgcca aaggggaagc tgaagccgca cgcagtaaag ctcaggcatt
     1621 gctgaaagaa accagggcgc gaatgcatgg tcgtactcgc gtccagcaag ccgcacgcga
     1681 tgccgttggc tgcgcggatt cttttgttcg tgaaagaccc tggtgtagcg tgggtacagc
     1741 agctgcggta ggtattttta tcggcgcact gttaagcatg cgcaaatcgt agtgcaaaaa
     1801 tgataataaa tacgcgtctt taaccccgaa gcctgtcttc ggggtttctt tttgcctggt
     1861 gaatcacaaa aatccccctc ccccgtcacg ctcatatcta gggtaatttc gatcactatt
     1921 tgctatatat tgtgtggttg aatctttttt caactacatc tagtatctct gtatcaacag
     1981 agagacaacc cgacgcgtat catcgcgccg tatcttcatt ttaaacggaa atacgaatca
     2041 tgcgcataac tatttacact cgtaacgatt gcgttcagtg ccacgccacc aaacgggcga
     2101 tggaaaaccg gggctttgat tttgaaatga ttaatgtcga tcgcgttcct gaagcagcgg
     2161 aagcgttgcg tgctcagggc tttcgtcagt tgccggtagt gattgctggc gatcttagct
     2221 ggtctggttt ccgtccggac atgattaacc gtctgcatcc agcgccacac gcggccagtg
     2281 catgagccag ctcgtctact tctccagcag ctccgaaaac acgcagcgtt ttatcgaacg
     2341 tttaggtctg cccgcggtgc gcatcccgct caatgagcgg gaacggattc aggtagacga
     2401 gccttacatc ctgatcgtgc cctcttacgg cggcggcggt acggctggcg cggtgccacg
     2461 ataggtaatt cgctttttaa acgacgagca caaccgggcg ttgcttcgcg gcgttattgc
     2521 ttctggtaat cgcaactttg gtgaggcgta tggccgcgcc ggagatgtga ttgcccggaa
     2581 atgcggcgtg ccgtggctgt accgttttga actcatgggt acgcaaagcg atatcgaaaa
     2641 cgttcgtaaa ggagtaaccg aattttggca acgacaaccg cagaatgcct gacgcaggaa
     2701 acgatggatt accacgcgct gaatgcgatg cttaacctct acgatagcgc aggtcgcatt
     2761 cagttcgata aagaccgcca ggccgttgac gcctttatgg cgacgcatgt gcgtccgaac
     2821 agtgtgacct tcagtagcca gcagcagcgc ctgaactggc tggtcaacga aggttactat
     2881 gatgaaagcg ttcttaatcg ctactctcgc gactttgtca ttacgctgtt tgcccacgca
     2941 cacaccagcg gttttcgttt ccagacattc ctcggggcat ggaagtttta caccagctat
     3001 acgttgaaga cattcgacgg taaacgttat ctggaagatt ttgccgatcg agtcacgatg
     3061 gtggcgctga cgctggcaca aggcgatgcg acgctggcgt tgcaactgac cgatgaaatg
     3121 ctgtcaggac gctttcagcc agccacgccg acattcctca actgcggtaa gcagcagcgc
     3181 ggcgaactgg tttcctgttt tctgctgcgt attgaagaca atatggagtc gattggccgg
     3241 gcggtaaatt ccgcactgca actgtcgaaa cgcggcggcg gcgtggcatt tttgctgtcg
     3301 aatctgcgag aagcgggcgc gccaattaaa cgtattgaaa atcaatcttc tggcgtaatt
     3361 ccggtgatga aaatgctgga agacgcattt tcctatgcca accaactcgg cgcgcgtcag
     3421 ggggcaggtg cagtctattt acatgctcat catcccgata ttctgcgttt tctcgataca
     3481 aaacgggaaa atgccgacga aaaaatccgc attaaaacac tgtcgctggg cgtggtgatc
     3541 ccggatatca ctttccatct ggcaaaagag aatgcgcaga tggcgctgtt ttcgccctat
     3601 gacgttgagc gagtttatgg caagccgttt gccgatatcg ccatcagcga acactatgac
     3661 gaactggttg ccgatgaacg catccgcaaa aaatacctca acgcccgtga tttcttccag
     3721 cggctggcag aaatccagtt tgagtccggc tatccctaca tcatgtatga agacacggta
     3781 aaccgtgcta accctgtcgc cgggcgcata aatatgagta atctctgctc agagattttg
     3841 caggtcaact gtgcctcaga gtatgacgag aatctcgact atgcccgcac aggccatgat
     3901 atctcctgca atttaggttc gttgaatatt gcgcacacca tggattcccc cgattttgcc
     3961 cgcacggtag agactgccgt gcgcggttta acggcggtgt cagatatgag tcatatccgc
     4021 agcgtgccgt ccatcgaagc cggaaatgcc gcctcacacg ccatcggact ggggcagatg
     4081 aatttacacg gctatctggc gcgagaaggc atcgcttatg gttcgccgga agcactggat
     4141 ttcaccaatc tctatttcta tgccatcacc tggcacgcac tgcgtacctc gatgttgctg
     4201 gcgcgcgaac gcggtgaaac cttcgccggg ttcaaacagt cacgttatgc cagtggtgaa
     4261 tattttagcc aatatctgca aggtaactgg cagccgaaaa cggcgaaagt tggcgaactg
     4321 tttgcccgca gcggtattac gttacctacc cgtgagatgt gggcgcaact gcgcgacgac
     4381 gtgatgcgct acggcattta caaccagaat cttcaggcgg tgccgccaac cggttctatc
     4441 tcttatatca accatgctac gtcgagtatt catccgattg tggcgaaagt agagatacgc
     4501 aaagagggca aaacaggacg cgtttactac cctgccccgt ttatgactaa cgaaaatctg
     4561 gcgctgtatc aggatgctta cgaaatcggc gcagaaaaga tcatcgacac ctacgcggaa
     4621 gcgactcgcc atgtcgatca gggtttgtcg ctgacgctgt ttttccccga taccgccacc
     4681 actcgcgata tcaacaaagc gcagatttac gcctggcgca aaggtatcaa aacgctctat
     4741 tacattcgcc tgcgtcagat ggcgctggaa ggcactgaaa ttgaaggctg cgtctcctgt
     4801 gcactttaag gaatatctat gaaactctca cgtatcagcg ccatcaactg gaacaagata
     4861 tctgacgata aagatctgga ggtgtggaat cgcctgacca gcaatttctg gctaccagaa
     4921 aaggtgccgc tgtcgaacga tattcctgcc tggcagacat taactgtcgt agaacaacaa
     4981 ctgacgatgc gcgtttttac tggcctgacg ctgctcgaca cgctgcaaaa tgttatcggc
     5041 gcgccttcgc tgatgcccga tgcactcacg cctcatgaag aagcggtatt atcgaatatc
     5101 agctttatgg aagcggttca tgcccgctct tacagttcga ttttctcgac gctgtgccag
     5161 accaaagatg tcgatgccgc ctacgcctgg agtgaagaaa acgcaccgtt gcagcgaaaa
     5221 gctcagatta ttcagcaaca ttatcgcggt gatgatccgc tgaaaaagaa aatcgccagt
     5281 gtgtttcttg aatctttttt gttctattcc ggtttctggc tgccgatgta tttttccagc
     5341 cgcggaaagc tgaccaatac cgcagacctg atccgtctga ttatccgtga tgaagcagtc
     5401 cacggttact acataggcta taaatatcag aaaaacatgg aaaagatatc tctgggacaa
     5461 cgtgaagagt tgaagagttt cgccttcgat ttgttgctgg aactctacga caacgagctg
     5521 caatacaccg atgagctgta cgccgaaacc ccgtgggctg acgatgtgaa agcgtttctc
     5581 tgttacaacg ccaataaggc tttgatgaat ctgggctacg aacctttatt tcccgcagaa
     5641 atggcggaag tgaatccggc aatcctcgcc gcgctttcgc cgaatgccga tgaaaatcac
     5701 gatttctttt ccggttcagg ctcctcttat gtgatgggga aagcggttga aacagaagat
     5761 gaagactgga atttctgagg gtgttatttt caaaaatatc actacccgcg acagggaaat
     5821 aattcccgcc aaatagcttt ttatcacgca aatattttgt ggtgatctac actgatactc
     5881 tgttgcatta ttcgcctgaa accacaatat tcaggcgttt tttcgctatc tttgacaaaa
     5941 aatatcaact tttctcgatt tgctctcagc ccttatatca cgggaaattc cggcgatttg
     6001 ctcgcatcaa tattcatgcc acatttgcca tcaggggttg cctcagattc tcagtatgtt
     6061 agggtagaaa aaagtaacta tttccattgg gtaatatatc gacataaaca aataacagga
     6121 atctttctat tgcatggcaa ttaaattaga aattaaaaac ctttataaaa tatttggtga
     6181 gcatccacag cgagcattca aatatatcga acaaggactt tcaaaagaac aaattctgga
     6241 aaaaactggg ctatcgcttg gcgtaaaaga cgccagtctg gccattgaag aaggcgagat
     6301 atttgtcatc atgggattat ccggctcggg taaatccaca atggtacgcc ttctcaatcg
     6361 cctgattgaa cccacccgcg ggcaagtgct gattgatggt gtggatattg ccaaaatatc
     6421 cgatgccaaa ctccgtgagg tgcgcagaaa aaagattgcg atggtcttcc agtcctttgc
     6481 cttaatgccg catatgaccg tgctggacaa tactgcgttc ggtatggaat tggccggaat
     6541 taatgccgaa gaacgccggg aaaaagccct tgatgcactg cgtcaggtcg ggctggaaaa
     6601 ttatgcccac agctacccgg atgaactctc tggcgggatg cgtcaacgtg tgggattagc
     6661 ccgcgcgtta gcgattaatc cggatatttt attaatggac gaagccttct cggcgctcga
     6721 tccattaatt cgcaccgaga tgcaggatga gctggtaaaa ttacaggcga aacatcagcg
     6781 caccattgtc tttatttccc acgatctcga tgaagccatg cgtattggcg accgaattgc
     6841 cattatgcaa aatggtgaag tggtacaggt cggcacaccg gatgaaattc tcaataatcc
     6901 ggcgaatgat tatgtccgta ccttcttccg tggcgttgat attagtcagg tattcagtgc
     6961 gaaagatatt gcccgccgga caccgaatgg cttaattcgt aaaacccctg gcttcggccc
     7021 acgttcggca ctgaaattat tgcaggatga agatcgcgaa tatggctacg ttatcgaacg
     7081 cggtaataag tttgtcggcg cagtctccat cgattcgctt aaaaccgcgt taacgcagca
     7141 gcaaggtctt gatgcggcgc tgattgatgc gccgttagca gtcgatgcac aaacgcctct
     7201 tagcgagttg ctctctcatg tcggacaggc accctgtgcg gtgcccgtgg tcgacgagga
     7261 ccaacagtat gtcggcatca tttcgaaagg aatgctgctg cgcgctttag atcgtgaggg
     7321 ggtaaataat ggctgatcaa aataatccgt gggataccac gccagcggcg gacagtgccg
     7381 cgcaatccgc agacgcctgg ggtacaccga cgactgcacc gactgacggc ggtggtgctg
     7441 actggctgac cagtacgcct gcgccaaacg tcgagcattt taatattctc gatccgttcc
     7501 ataaaacgct gatcccgctc gacagttggg tcactgaagg gatcgactgg gttgttaccc
     7561 atttccgtcc cgtcttccag ggcgtgcgcg ttccggttga ttatatcctc aacagtttcc
     7621 agcaattgct gctgggtatg ccagcgccag tggcgattat cgttttcgct ctcatcgcct
     7681 ggcagatttc cggggtcgga atgggcgtgg cgacgctggt ttcgctgatt gccatcggcg
     7741 caatcggtgc ctggtcgcag gcaatggtga ctctggcgct ggtgttaacc gccctgctgt
     7801 tctgtatcgt catcggtttg ccgttgggga tatggctggc gagaagtccg cgagcggcga
     7861 aaattattcg tccactgctt gatgccatgc agaccacgcc agcgtttgtt tatctggtac
     7921 caatcgtcat gctgtttggt atcggtaacg tgccgggcgt agtggtgacg atcatctttg
     7981 ctctgccgcc gattatccgt ctgacgattc tggggattaa ccaggttccg gcggatctga
     8041 ttgaagcctc gcgctcattc ggtgccagcc cgcgccagat gctgttcaaa gttcagttac
     8101 cgctggcgat gccgaccatt atggcgggcg ttaaccagac gctgatgctg gctctttcta
     8161 tggtggtcat cgcctcgatg attgccgtcg gcgggctggg tcagatggta cttcgcggta
     8221 tcggtcgtct ggatatgggc cttgccaccg ttggcggcgt cgggattgtg atcctcgcca
     8281 ttatcctcga ccgcctgacg caggccgttg ggcgcgactc acgcagtcgc ggcaaccgtc
     8341 gctggtacac cactggccct gtcggtctgc tgacccgccc attcattaag taactctgca
     8401 cttgcccggt aacgccgggc attatcaccc tgccaaaaaa aggaataaca atgcgacata
     8461 gcgtactttt tgcgacagcg tttgccacgc ttatctctac acaaactttt gcagccgatc
     8521 tgccgggcaa aggcattact gttaatccag ttcagagcac catcactgaa gaaaccttcc
     8581 agacgctgct ggtcagtcgt gcactggaga aattaggtta taccgtcaat aaacccagcg
     8641 aagtggatta caacgttggc tacacctcac ttgcttccgg cgatgcaacc ttcaccgccg
     8701 tgaactggac gccgctgcat gacaacatgt acgaagctgc cggtggcgat aagaaatttt
     8761 attgtgaagg ggtattcgtt aacggcgcgg cacagggtta cctgatcgat aagaaaaccg
     8821 ccgaccagta caaaatcacc aacatcgcac aactgaaaga tccgaagatc gccaaactgt
     8881 tcgataccaa cggcgacggt aaagcggatt taaccggctg taacccaggc tggggctgcg
     8941 aaggtgcgat caaccaccag cttgccgcgt atgaactgac caacaccgtg acgcataatc
     9001 aggggaacta cgcagcgatg atggccgaca ccatcagtcg ctacaaagag ggcaaaccgg
     9061 tgttttatta cacctggacg ccgtactggg tgagtaatga gctgaagcca gggaaagatg
     9121 tggtctggtt gcaggtgccg ttctccgcac tgccgggcga taaaaacgcc gataccaaac
     9181 tgccgaatgg tgcgaattat ggcttcccgg tcagcaccat gcatatcgtt gccaacaaag
     9241 cctgggccga gaaaaacccg gcagcagcga aactttttgc cattatgcag ttgccagtgg
     9301 cagatattaa cgcccagaac gccattatgc atgacggcaa agcctcagaa ggcgatattc
     9361 agggccatgt tgatggttgg atcaaagccc accagcagca gttcgatggc tgggtgaatg
     9421 aggcgctggc agcgcagaag taatttttat tcgggcggat aagtcgtttt tgcctcatcc
     9481 gccattctgt gcacaatgcc tgatgcgacg ctggcgcgtc ttatcatgcc tacaaaatac
     9541 actcattccg tatggcggat aaggcgtttt cgccgcatcc gccgttctgt gcacaatgcc
     9601 tgatgcgacg ctggcgcgtc ttatcatgcc tacaaaccac atcgcacatt tcaacaagct
     9661 atctttcgcc cccattcatc aacatccgct attattgatt tccagcttaa tcatcacctg
     9721 atgaacaaaa ataatgacta aacctaatca tgagcttagc ccggcgctga tcgtgctgat
     9781 gtctatcgcc accggtcagg cggtagccag taactattac gcccagccat tgctcgacac
     9841 catcgcgcgt aacttttccc tttccgccag ttcggcaggc tttattgtta ccgccgcgca
     9901 gttgggctat gcagcaggtc tgctgtttct tgttcccctc ggtgatatgt ttgaacgccg
     9961 ccgcctgatt gtctcgatga ccttactggc ggcaggcggc atgttaatca ctgccagcag
    10021 tcagtcgctg gcgatgatga tcctcggtac ggcattaacc ggtttattct cagtcgtggc
    10081 acaaattctg gttccgctgg cagcgacgct ggcttcaccg gataaacgcg gcaaagtggt
    10141 tggcaccatt atgagcggtc tgctgttggg gatcttgctg gcacgaaccg ttgccgggtt
    10201 actggcaaac ctcggcggct ggcgcaccgt cttttgggtt gcctcggtgt taatggcact
    10261 gatggcgcta gcattatggc gtggtctgcc acaaatgaaa tcagaaaccc acctcaacta
    10321 cccacagttg ttaggttccg ttttcagcat gtttatcagc gataagatcc tccgcacccg
    10381 cgcattgctg ggctgcctga cctttgccaa cttcagcatt ctctggacct caatggcctt
    10441 tttgcttgcc gctccacctt ttaactacag cgatggtgtc attggtctgt ttggacttgc
    10501 gggagctgcc ggagcgttgg gcgctcgtcc ggcgggcggt tttgccgata agggtaaatc
    10561 gcaccacacc acaactttcg gtctgctgct gctattactt tcatggctgg cgatctggtt
    10621 tggtcacact tccgtactgg cgctgattat cggcatcctg gtgctggatc tcaccgtaca
    10681 gggcgtgcat atcactaacc agacggtaat ttatcgaata caccctgatg cgcgtaatcg
    10741 cctgaccgca ggttacatga ccagctactt tattggcggt gccgccggtt cgctaatttc
    10801 agcctcagcc tggcaacatg gcggttgggc tggcgtttgt ctggctggcg cgacgattgc
    10861 cctagttaac ttactggtct ggtggcgagg ttttcatcgt caggaagccg caaattaagc
    10921 aatcagcaac cgttttcgtg gctttacact tataagggtg ttaagaagcc catcagtctg
    10981 ataaggttaa gatattcatt cagtctattt ataatattaa caatcgttaa gcgtacactc
    11041 tatggaaagc cctactccac agcctgctcc tggttcggcg accttcatgg aaggatgcaa
    11101 agacagttta ccgattgtta ttagttatat tccggtggcc tttgcgttcg gtctgaatgc
    11161 gacccgtctg ggattctctc ctctcgaaag cgtttttttc tcctgcatca tttatgcagg
    11221 cgcgagccag ttcgtcatta ccgcgatgct ggcagccggg agtagtttgt gggttgctgc
    11281 actgaccgtc atggcaatgg atgttcgcca tgtgttgtat ggcccgtcac tgcgtagccg
    11341 tattattcag cgtctgcaaa aatcgaaaac cgccctgtgg gcgtttggcc tgacggatga
    11401 ggtttttgcc gccgcaacag caaaactggt acgcaataat cgccgctgga gcgagaactg
    11461 gatgatcggc attgccttca gttcatggtc atcgtgggta tttggtacgg taataggggc
    11521 attctccggc agcggcttgc tgcaaggtta tcccgccgtt gaagcagcat taggttttat
    11581 gcttccggca ctctttatga gtttcctgct cgcctctttc cagcgcaaac aatctctttg
    11641 cgttaccgca gcgttagttg gtgcccttgc aggcgtaacg ctattttcta ttcccgtcgc
    11701 cattctggca ggcattgtct gtggctgcct cactgcgtta atccaggcat tctggcaagg
    11761 agcgcccgat gagctatgag gttctgctgc ttgggttact ggttggcgcg gcgaattatt
    11821 gcttccgcta tttgccgctg cgcctgcgtg tgggtaatgc ccgcccaacc aaacgtggcg
    11881 cggtaggtat tttgctcgac accattggca tcgcctcgat atgcgctctg ctggttgtct
    11941 ctaccgcacc agaagtgatg cacgatacac gccgtttcgt gcccacgctg gtcggcttcg
    12001 cggtactggg tgccagtttc tataaaacac gcagcattat catcccaaca ctgcttagtg
    12061 cgctggccta tgggctcgcc tggaaagtga tggcgattat ataacccaca agaatcattt
    12121 ttctaaaaca atacatttac tttatttgtc actgtcgtta ctatatcggc tgaaattaat
    12181 gaggtcatac ccaaatggat agttcgttta cgcccattga acaaatgcta aaatttcgcg
    12241 ccagccgcca cgaagatttt ccttatcagg agatccttct gactcgtctt tgcatgcaca
    12301 tgcaaagcaa gctgctggag aaccgcaata aaatgctgaa ggctcagggg attaacgaga
    12361 cgttgtttat ggcgttgatt acgctggagt ctcaggaaaa ccacagtatt cagccttctg
    12421 aattaagttg tgctcttgga tcatcccgta ccaacgcgac gcgtattgcc gatgaactgg
    12481 aaaaacgcgg ttggatcgaa cgtcgtgaaa gcgataacga tcgccgctgc ctgcatctgc
    12541 aattaacgga aaaaggtcac gagtttttgc gcgaggtttt accaccgcag cataactgcc
    12601 tgcatcaact ctggtccgcg ctcagcacaa cagaaaaaga tcagctcgag caaatcaccc
    12661 gcaaattgct ctcccgtctc gaccagatgg aacaagacgg tgtggttctc gaagcgatga
    12721 gctaacgcgt catctcgctc aaaaatccag atttataaaa gaaaaaatga ccggccagca
    12781 tcgcaacatg ctggcctttt tggcaagcag gtcggctcag ccgatgagtt aagaagatcg
    12841 tggagaacaa tatgagcgca aatgcggaga ctcaaacccc gcagcaaccg gtaaagaaga
    12901 gcggcaaacg taagcgtctg ctcctccttc tcaccttgct ctttataatt attgccgtag
    12961 cgatagggat ttattgtttt ttggtactgc gtcatttcga agaaaccgat gacgcatacg
    13021 tggcagggaa tcagattcaa attatgtctc aggtgtctgg cagcgtgacg aaagtctggg
    13081 ccgataacac cgattttgta aaagaaggcg acgtgctggt cactctcgac ccgacagatg
    13141 ctcgccaggc gtttgaaaaa gccaaaactg cactggcttc cagcgttcgc caaacccacc
    13201 agctgatgat taacagcaaa cagttgcagg cgaatattga ggtgcagaaa atcgccctcg
    13261 caaaagcaca aagcgactac aaccgccgtg tgccgctggg caatgccaac ctgattggtc
    13321 gcgaagagct gcaacacgcc cgcgacgccg tcaccagtgc ccaggcgcaa ctggacgtcg
    13381 ctattcaaca atacaatgcc aatcaggcga tgattctggg gactaaactg gaagatcagc
    13441 ctgccgtgca acaggctgcc accgaagtac gtaacgcctg gctggcgctg gagcgtaccc
    13501 gtattgtcag tccgatgacc ggttatgtct cccgccgcgc ggtacagcct ggggcgcaaa
    13561 ttagcccaac gacgccgctg atggcggtcg ttccagccac caatatgtgg gtggatgcca
    13621 actttaaaga gacgcagatt gccaatatgc gtatcggtca gccggtcact atcaccacgg
    13681 atatttacgg cgatgatgtg aaatacaccg gtaaagtggt tggtctggat atgggcacag
    13741 gtagcgcatt ctcactgctt ccggcgcaaa atgcgaccgg taactggatc aaagtcgttc
    13801 agcgtctacc tgtgcgtatc gaactggacc agaaacagct ggagcaatac ccgctgcgca
    13861 ttggtttgtc cacactggtg agcgtcaata ccactaaccg tgacggtcag gtactggcaa
    13921 ataaagtacg ttcaacaccg gtcgcggtaa gtaccgcgcg tgaaatcagc ctggcacctg
    13981 tcaataaact gatcgacgat atcgtaaaag ctaacgctgg ctaatccaga ggtgcgtgtg
    14041 atgcaacagc aaaaaccgct ggaaggcgcg caactggtca ttatgacgat tgcgctgtca
    14101 ctggcgacat tcatgcaggt gctggactcc accattgcta acgtggcgat ccccactatc
    14161 gccgggaatc tgggctcatc gctcagccag ggaacgtggg taatcacttc tttcggggtg
    14221 gcgaatgcca tctcgatccc gcttaccggc tggctggcaa agcgcgtcgg ggaagtgaaa
    14281 ctgttccttt ggtccaccat cgcctttgct attgcgtcgt gggcgtgtgg tgtctccagc
    14341 agcctgaata tgctgatctt cttccgcgtg attcagggga ttgtcgccgg gccgttgatc
    14401 ccgctttcgc aaagtctatt gctgaataac tacccgccag ccaaacgctc gatcgcgctg
    14461 gcgttgtggt cgatgacggt gattgtcgcg ccaatttgcg gcccgatcct cggcggttat
    14521 atcagcgata attaccactg gggctggata ttcttcatca acgtgccgat tggcgtggcg
    14581 gtggtgttga tgacactgca aactctgcgc ggacgtgaaa cccgcaccga acggcggcgg
    14641 attgatgccg tggggctggc actgctggtt attggtatcg gcagcctgca gattatgctc
    14701 gaccgcggta aagagctgga ctggttttca tcacaggaaa ttatcatcct taccgtggtg
    14761 gcggtggtgg ctatctgctt cctgattgtc tgggagctga ccgacgataa cccgatagtc
    14821 gatctgtcgt tgtttaagtc gcgcaacttc accatcggct gcttgtgtat cagcctcgcg
    14881 tatatgctct acttcggcgc tattgttctg ctgccgcagt tgttgcagga ggtctacggt
    14941 tacacggcga cctgggcagg tttggcctct gcgccggtag ggattattcc ggtgatcctg
    15001 tcgccgatta tcggccgctt cgcgcataaa ctggatatgc ggcggctggt aaccttcagc
    15061 tttattatgt atgccgtctg cttctactgg cgtgcctata cctttgaacc aggtatggat
    15121 tttggcgcgt cggcctggcc gcagtttatc caggggtttg cggtggcctg cttctttatg
    15181 ccgctgacca ccattacgct gtctggtttg ccaccggaac gactggcggc ggcatcgagc
    15241 ctctctaact ttacgcgaac gctggcgggg tctatcggca cgtcgataac cacgaccatg
    15301 tggaccaacc gcgagtcgat gcaccatgcg cagttgactg agtcggtaaa cccgttcaac
    15361 ccgaatgccc aggcgatgta cagtcaactg gaagggcttg ggatgacgca acagcaggca
    15421 tcaggctgga ttgcccagca gatcaccaat caggggctga ttatttccgc caatgagatc
    15481 ttctggatgt cagccgggat attcctcgta ctgctggggc tggtgtggtt tgctaaaccg
    15541 ccatttggcg caggtggcgg tggcggcggt gcgcactaag tacaactaag ccagttcatt
    15601 tgaactggct tttttcaatt aattgtgaag atagtttact gactagatgt gcagttcctg
    15661 caacttctct ttcggcagtg ccagttcttc gttgctgttg atgcgtacgt cacgttccag
    15721 aatgctacgc gcaatatcct gcgcttcctg caacgagtgc atctggtaag tgccacactg
    15781 gtagacgttc agttccggga tctgattctg atcctgcact ttcagcacgt cttccattgc
    15841 cgctttccag gcatcagcaa cacgctgctc atctggcgta ccaatcagac tcatataaaa
    15901 accggtgcgg cagcccattg gcgagatatc gataatctct acaccattac cgttaagatg
    15961 gttacgcata aaaccagcaa acaggtgctc cagggtatgg atccctcttt ctggcatcac
    16021 ttctttgttc ggcacgcaga agcgcagatc gaacacggtg attgcgtcgc catgcggggt
    16081 gttcattgtt ttcgccaccc gaactgcagg cgcttccatc cgggtatgat cgactgtgaa
    16141 gctatctaac aacggcattt agccacctcc ggtaattttt ttaaaaattt tctgaactct
    16201 ttcttcccag gcgagtctga gtatatgaaa gacgcgcatt tgttatcatc atccctgaat
    16261 tcagagatga aattttggcc actcacgagt ggcctttttc ttttctgtca ggtgtgtttt
    16321 tccagccaca ccgcaaacgg ttcggtatca gcggtttcca tttcctgctg acggcgttca
    16381 gatgcctcgc gctcggctac aaaatcctct tcgcgcagaa tttccagcgg ctcttcacgc
    16441 agcagattac ggtaggcttc tgcaaacgct ttgcctgttc cgccaatacc agtatcaatc
    16501 atagacctta agatacgggc agagaaagtc agatcgggat tatcgaagca ggcaaccagt
    16561 tcatcacaca ctttctgata cgcgtcgccg ccgttaatgc tatccagcgt ttgcgcgacg
    16621 cgtttcagat cgcggaacag atctttaccc acctgcagta acgggaactg cgcggtttcg
    16681 cagccgatac ccagcgtcag gcccggtttg cgaccttcaa ggatcacccg gttccagtta
    16741 acgcgcgtac aggcaagttc gctactgctc atttccggtg catcagccag cgcacaccag
    16801 accataaaca ggtcgaggaa tcgcacctgc tgttcatcta caccaatcgg cgagaacggg
    16861 ttgatgtcca gcgaacgcac ttcaatatat tcaatgccgc cacgtaacag cgcatcagaa
    16921 ggtgactcgc cgctgcgggt aacgcgtttt ggacgaatcg gcgcatacag ttcgttttca
    16981 atctgcagca cgttgctatt gatttgcagc ctcttaccgt ctttctcaat accaatcttc
    17041 gcgtactctt ccgatggcgt tttgattgcc tgtttaaggc ccgctacgta ctcgtaaaga
    17101 tcgttgaagg taataccaag attgctttgc gatttattgg tatagccgag atcgctcaaa
    17161 cgaagagagg tcgcatacgg caggtaatac ataccgcact cggttttctc aaacggcagc
    17221 gacgttggtt ttccttgcag gaaagaagaa caaatcgccg gagatgcacc aaacagataa
    17281 ggaatgaccc aaccgaaacg atagtaattg cggataacgc ggaaatagcc cgcagaaatt
    17341 ttctctttgg catcagcgcc cgagatatca ccgcacttcg cttgccagaa tgccattggc
    17401 aaagagaaat tgtagtgcac gccggaaatg gtttgcatca gcgcgccgta gcgatttttc
    17461 agcccttcac gatacagcgt tttaaagcgt ccggtgttag aagtgccgta ctgtgccagt
    17521 tcgatgtcct gaccttctgc gatgtagcat ggcatactta acggccacat ccgctcatcg
    17581 cccatattgc gcgccgtata acgatgcaga tcgcgcataa aggtcagcat atgttcaata
    17641 tcaccatcca ctggtgtaat gaattccagc aatgcttccg caaaatcggt agtaatccat
    17701 ttgtgcgtca gtgcggaacc taatgcttca ggatgacctg ttgttgccag tgtgccatca
    17761 gcattaacac gcaaagtttc gcgctccagc ccacgctgta tcccctttaa cgcctgagga
    17821 tgtttttcca gccaggccag cgcctgtgat acgtccggga tcaaattgac ctcccgcctg
    17881 tcaaaatcgt tttaattagc ataactgtaa tggtgaccat atgtgcaggc ctacaattag
    17941 tgccaccaca tcatgccctg aacggtcgct gctgcaactg caacatagcg taacgcttta
    18001 ccaaggcaca aaaaaaagat taccggtccc cacgagatgc gcatccatcc cgctaacaga
    18061 cacagtaaat cgccaaccac gggcatccag cttaataata gcgtgactgc accatagcgt
    18121 ttcagccagc cgatagcttt ctcttgccag cgcgatgttt tacgcaatgg aaagaaacgc
    18181 ccaaggataa cgttagttaa ccctccaagg ctattaccca ttgttgctgt taaaactaaa
    18241 acccagggat gactgatccc ggaaagcaac attgccacca gcacgacttc ggagttgccg
    18301 ggtaatagtg tagcgctgag aaaactactg gcaaacaacg agaaaagcga taacgcttca
    18361 ctcacagcaa gcgaacgtcc acagcgtcca tacctgcggc acgggccgcc tgaataccga
    18421 aatcggcatc ttcaaagacc acacactgcg tcggttgcac gcccatacgc tgcgcgcaca
    18481 acaaaaatgt gtctggcgcg ggtttatggt gtttgacgtg atcggcagcg acgacggcgt
    18541 caaaataatg gcgtaatccc aggtgcgcca gcaatgcctc agcgatggcg ctttcactcc
    18601 ccgttcctac agccattggg cgacgaccat gccaactttt caccacatca acaagaggaa
    18661 gcggttcgac gctatccagc agcatacttc ttactgcttc tgttttttca cgcgctaacg
    18721 catgcgggtc gagatcggcc tgattcagct caataattgc ctgagcaata cgccaggtgg
    18781 gcgatccatt aagcgcaatc atcgcctgaa tatcgtactg aagaccgtag tgccctaata
    18841 cttcgcgcca cgctttacgg tgcgtaggct ccgtatccag gattgtgcca tccatatcaa
    18901 aaattaaacc tgcataacgc tcgtacatgg tcttctcgca agtcgaaaaa tatgacgcta
    18961 ctttagcgta attgcttgat tttgtcgctg atggcgaagg agaagaaaat tcaggaaatg
    19021 gataaaggag ttatttagaa atgagatatt tttgaaggaa attttttgga gaagatggtg
    19081 catccgggag gatgactcac tgacgttcgc ccttcgggcc gttgctggcg caacgttatc
    19141 cttcctggtg cctgcggtta ataccgctat ctcttcgata ccttcattgc tgaaggttac
    19201 ggagaatatg gtgcatccgg gaggattcga acctccgacc gctcggttcg tagccgagta
    19261 ctctatccag ctgagctacg gatgcatcgg gaaaacttat tctactgctg atattgatac
    19321 cgctactaaa gccatatcaa ttaagagatg gtgcatccgg gaggattact cgccttgcgg
    19381 ctcgcccttc gggccgttgc taaagcaacg ttatcatccc tggtgcctgc ggttaattcc
    19441 gctacctctt cgataccttc attgctgaag atttcggaga a
//
LOCUS       DAESHI010000078        19372 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.079, whole genome shotgun sequence.
ACCESSION   DAESHI010000078 DAESHI010000000
VERSION     DAESHI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19372
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.079"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(31..1860)
                     /gene="glmS"
                     /locus_tag="KW011_002840"
     CDS             complement(31..1860)
                     /gene="glmS"
                     /locus_tag="KW011_002840"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBH7661127.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="KW011_002841"
     CDS             complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="KW011_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBH7661128.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3743..4162)
                     /gene="atpC"
                     /locus_tag="KW011_002842"
     CDS             complement(3743..4162)
                     /gene="atpC"
                     /locus_tag="KW011_002842"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBH7661129.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="KW011_002843"
     CDS             complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="KW011_002843"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBH7661130.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="KW011_002844"
     CDS             complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="KW011_002844"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBH7661131.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="KW011_002845"
     CDS             complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="KW011_002845"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBH7661132.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="KW011_002846"
     CDS             complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="KW011_002846"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBH7661133.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="KW011_002847"
     CDS             complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="KW011_002847"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBH7661134.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="KW011_002848"
     CDS             complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="KW011_002848"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HBH7661135.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="KW011_002849"
     CDS             complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="KW011_002849"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBH7661136.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="KW011_002850"
     CDS             complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="KW011_002850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HBH7661137.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11247..11870)
                     /gene="rsmG"
                     /locus_tag="KW011_002851"
     CDS             complement(11247..11870)
                     /gene="rsmG"
                     /locus_tag="KW011_002851"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBH7661138.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11934..13823)
                     /gene="mnmG"
                     /locus_tag="KW011_002852"
     CDS             complement(11934..13823)
                     /gene="mnmG"
                     /locus_tag="KW011_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBH7661139.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14202..14645)
                     /gene="mioC"
                     /locus_tag="KW011_002853"
     CDS             complement(14202..14645)
                     /gene="mioC"
                     /locus_tag="KW011_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HBH7661140.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14735..15193)
                     /gene="asnC"
                     /locus_tag="KW011_002854"
     CDS             complement(14735..15193)
                     /gene="asnC"
                     /locus_tag="KW011_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HBH7661141.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15345..16337
                     /gene="asnA"
                     /locus_tag="KW011_002855"
     CDS             15345..16337
                     /gene="asnA"
                     /locus_tag="KW011_002855"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBH7661142.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(16342..17793)
                     /gene="viaA"
                     /locus_tag="KW011_002856"
     CDS             complement(16342..17793)
                     /gene="viaA"
                     /locus_tag="KW011_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HBH7661143.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMHIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17787..19283)
                     /gene="ravA"
                     /locus_tag="KW011_002857"
     CDS             complement(17787..19283)
                     /gene="ravA"
                     /locus_tag="KW011_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HBH7661144.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQQDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
BASE COUNT         4630 a         5123 c         4849 g         4770 t
ORIGIN      
        1 tcggcagccc ttttttatct atgatagtga ttactcaacc gtaaccgatt ttgccaggtt
       61 acgcggctgg tcaacgtcgg tgcctttgat cagcgcgaca tggtaagcca gcagctgcag
      121 cggaacggtg tagaagatcg gtgcaatcac ctcttccaca tgcggcatct cgatgatgtg
      181 catgttatcg ctacttacaa aacccgcatc ctgatcggcg aagacataca attggccgcc
      241 acgcgcgcga acttcttcaa tgttggattt cagtttttcc agcaattcgt tgttcggtgc
      301 aacgacgata accggcatat cggcatcaat aagcgccagc ggaccgtgtt tcagttcacc
      361 agcagcgtag gcttcagcgt gaatgtagga gatctctttc agcttcaatg cgccttccag
      421 tgcgattggg tactgatcgc cacggcccag gaacagtgcg tgatgtttgt cagagaaatc
      481 ttctgccaga gcttcaatgc gtttgtcctg agatagcatc tgctcaatac ggctcggcag
      541 cgcctgcaga ccatgcacaa tgtcatgttc aatggaggca tccagacctt tcaggcgaga
      601 cagcttcgcc accagcatca acagcacagt taactgagtg gtgaatgctt tagtggacgc
      661 cacgccgatt tccgtacccg cgttggtcat cagcgccaga tcggattcgc gcaccagaga
      721 agaacccgga acgttacaga ttgccagcga accaaggtaa cccagctctt tcgacagacg
      781 caggcccgcc agggtatccg cggtttcgcc agactgtgac aaggtgatca tcaggctgtt
      841 acgacgcacg gcagatttac gatagcggaa ttcagaggcg atttcgacgt cgcacggaat
      901 acctgccagc gattcaaacc agtagcggga aaccataccg gagttataag aagtaccaca
      961 ggcgaggatc tgaatatgct caaccttcga cagcagttcg tcggcgttcg gtcccagctc
     1021 gcttaaatca acctgaccgt ggctgatgcg cccggtaagg gtgtttttta tcgcgttcgg
     1081 ctgttcgtag atctctttct gcatgtagtg acggtaaata cctttatcgc ccgcgtcata
     1141 ttgcagattg gattcgatat cctgacgttt tacttccgcg ccagttttat cgaagatgtt
     1201 taccgaacgg cgagtgattt ccgcaatatc gccctcttca aggaagataa agcgacgggt
     1261 caccggcaac agcgccagct ggtcagaagc gataaagttt tcgcccatac ccaagccaat
     1321 caccagcgga ctaccagaac gtgccgccag cagggtatcc gggtgacggg agtccatgat
     1381 cactgtaccg tacgcaccgc gcagctgcgg gatagcacgc agaacagcct cacgcagagt
     1441 cccgccttgt ttcagctccc agttcaccag atgggcaatc acttcggtgt cggtttcaga
     1501 aacgaaggta tagccacgcg cttttaactc ttcacgcagc ggttcatggt tttcgatgat
     1561 gccgttatgc accaccacaa tgtgttcaga aacatgcgga tgcgcgttag cttctgaagg
     1621 ttcaccgtgg gtcgcccagc gagtgtgagc aataccggtg ccgccatcca gaggatgttc
     1681 ttccgctgcc tgagccagca tctggacttt accgaggcga cgcaggcggg tcatatgacc
     1741 ttccgcatca acaacggcca gaccggcaga gtcatatccg cggtattcca gacgacgtaa
     1801 accttcaaga aggatttctg ctacatcacg ttgcgcgatc gcgccaacaa ttccacacat
     1861 agtttttgat tccgatttat atcgttgtcg gtcaacctgt atgcccgttt tttcgggcgc
     1921 cccgagcctt gtagagagtg gggttatttt tatagttact gcttgtggga gggagattat
     1981 gttatctcct catcccagat gaccggatta cccggccaga attacttttt ctttaccgga
     2041 cgacgccagc cttctttctg agtctgcggc acacggctga tagctaatgc attttcgccg
     2101 acattacgcg tcacagttgt acccgcagca atggtcgcgc ctttgcctac tgttaccggg
     2161 gccaccagct gagtgtcgga accgacaaac acatcgtcgc cgataatggt cttaaattta
     2221 ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga tgttaacgtt atcgccaatt
     2281 tccgcatcgc ccaggtaagt cagatgcccg actttcgagc ctttacccag acgcgctttt
     2341 ttcatctcaa cgaagttacc aacgtgtgca ccttccagca actcagcacc aggacgcaaa
     2401 cgagcaaacg ggccaatagt acaggccgct gccagattcg cgtcttccac aacggtatac
     2461 ggactgattt cgcaatcatc gccaatcacg ctgtttttaa tcacgcaacc ggtgccgatt
     2521 ttcacgcgat gaccgagagt cacgttgccc tcgatgataa cgttagtatc aatttcaaca
     2581 tcgcgcccgt gagtaagcgt accgcgcaga tcaaaacgcg ccggatcgcg cagcataacg
     2641 cctgctaaca gcagtttttc agcctgttcg gactggtaaa cgcgctccag acgggagagt
     2701 tgcaggcggt tattcacacc ttctacttcg cttaaacgtt gcggatgaac ggcgacgatt
     2761 tcacgccctt cctgatacgc cagcgcaata atgtcggtga tgtagtattc gccctgagca
     2821 ttattgttgg tcagcttcgc cagccagcgt ttcatatctg cgccgttagc gatcagaatg
     2881 ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg catctttgtg ctcaacaatg
     2941 ccggtaactt tgccattttc acgggtgata cgtccataac cggtcggatc atccagtttc
     3001 accgtcagca gaccaatgcc accctgcggt ttagcatcac gcaggcgctg gagtgtttcg
     3061 acagagatca gcggcacgtc gccgtagagc attaaaatgt cttcatcatc ggcaaagaaa
     3121 ggtgcggcct gctgcattgc atgacccgta cccagctgct ctgcctgaag cacccagtta
     3181 aggttgtcgt ctttcagcgc ctgtttaagc agatcgccgc cgtgaccgta caccaagtga
     3241 acgtgcgctg cgcctaattc attcgcagca tcaatgacat gctgaaccat cgctttcccg
     3301 gcaagggtat gcagcacttt cggaagatcg gaatacatgc gcgtgccttt gcctgcggca
     3361 aggatcacta cgctcatagc attattcaac atacgcgtcc tgactgtaat ttgagaacga
     3421 atttaaaccg cttcaccttg aaaaaactac atttttttca tcgtgaaatg gacagaggat
     3481 aaattgttca ataacggatt atccccgctg acgaaagtgc cattttcgac catcgtcacg
     3541 ccttttgtct gcagaaaatt aaggcagtac cttcatttaa gcgtcaggtg gatgtttttg
     3601 ctcttatttc gatcaatgaa taaacgggaa aacaggggtg ttttatatca gcaggatcta
     3661 tgtgaacgct cttcaggacg gatcacgcgt aaaaaaaagc cagcctgttt ccagactggc
     3721 ttttgtgctt ttcaagccgg tgttacatcg cttttttggt caactcgata acgcgcagct
     3781 gcgcgatcgc tttggccagt tccgcagacg cctgagcgta atctacgtcg ccgtgagagc
     3841 tgctaatgtg ctcttcagcc ttacgtttcg cttccatggc tcgcgcttcg tcgagatcct
     3901 ggccgcgaat tgcggtgtcg gccagaacgg tcacgttgcc aggctgcact tcaagaatgc
     3961 cgccagacag atagataaac tcttcgtgac cgtgctgttt cacgatgcga atcataccag
     4021 gcttaatggc ggtgagcagc ggtgcgtggc cagggtagat ccccagttca ccttcgctac
     4081 ccgttacctg gattttctcg accagaccag agaacatttg ttgctctgcg ctgacgacgt
     4141 ccaggtggta agtcattgcc atatcaccct ccgattaagg cgttaaagtt ttttggcttt
     4201 ttccacagct tcttcgatgg aaccgaccat gtagaacgcc tgctccggca ggtgatcgta
     4261 ttcgccttcc atgatgcctt taaagccacg gatggtgtct ttcagggaga cgtatttacc
     4321 cggagaaccg gtgaatactt ctgccacgaa gaacggctgg gacaggaagc gctggatctt
     4381 acgagcacgc gctaccacca gtttgtcttc ttcagacagt tcatccatac ccaagatggc
     4441 gatgatgtct ttcagttcct gataacgttg caggatggac tgaacgccac gcgcggtgtc
     4501 gtagtgttcc tgaccaacca ccagcgggtc cagctgacgg ctggtggagt ccagcgggtc
     4561 aacggccggg tagataccca gagacgcgat ctgacggctc agtaccacgg ttgcgtcaag
     4621 gtgcgcaaag gtggttgccg gagacgggtc agtcaagtca tccgcaggta cgtataccgc
     4681 ctgtacggag gtgatagaac cagttttggt ggaggtgata cgttcctgca gaacgcccat
     4741 ctcttccgcc agggtcggct gataacctac cgctgaaggc atacggccca gcagtgcgga
     4801 tacttccgta ccggccaggg tgtaacgata gatgttgtca acgaacagca gaacgtcacg
     4861 accttcgtca cggaatttct cagccatggt cagaccggtc agagcaacgc gcagacggtt
     4921 tcccggcggc tcgttcatct ggccatacac cagggatact ttgtcgataa cgttggagtc
     4981 ggtcatttcg tggtagaagt cgttaccctc acgagtacgt tcacctacgc ccgcaaacac
     5041 agagtaaccg gagtgctcga tcgcgatgtt acgaatgagc tccatcatgt ttacggtttt
     5101 acctacaccc gcaccaccga acagaccaac tttaccgccc ttagcgaacg gacacatcag
     5161 gtcgataact ttgataccgg tttccagcag ttcctgagag tttgacagct cttcgtagga
     5221 aggtgctgcg cggtgaatcg cccaacgctc ttcttcaccg atctcgcctt tcatgtcgac
     5281 cggttcaccc agtacgttca tgatacggcc cagagtcgct ttacctaccg ggacttcgat
     5341 cgggtgttcg aggtctttta catccagacc gcgacgcaga ccgtcggagg aacccattgc
     5401 gatggtacgc acgataccgc cgccgagctg ctgctgaact tccagcacca gacgctcatt
     5461 accattttgc acctcaagag catcgtacac gcgcggtacg gcatcctgag ggaattcgac
     5521 gtcaactacg gcgccgatta cctggacaat ctttccagta gccatcttaa atcctctacg
     5581 aaataacctg tttaaaccgc ggcggccccc gagacgatct cggtgagttc ctgagtaatg
     5641 ctggcctgac gagctttgtt gtataccaac tgcagctctt taatcaggct gccgccattg
     5701 tcggtcgcgg ctttcatcgc caccatacgg gcggcctgct cgctggccag gttttcaacc
     5761 acgccctgat aaacctgaga ttcgacataa cgacgcagca gggtatccag caacgccttc
     5821 ggatcgggtt cgtacaggta atcccaggat ttatgtttca gatcatcatc atctgatgcc
     5881 ggtaacggca gcagctggct gatggtcgga acctgagaca tggtgttaat aaatttgttg
     5941 ctgacaatgt aaagcttgtc cagacggcct tcgtcgtaag cctgcaacat cacttttacc
     6001 ggaccgatca gttcggacag ggaagggtta tcccccatgc cggtgacctg ggcaacaaca
     6061 ttgccgccca cggagttgaa gaacgacacg cctttcgagc cgatcattgc gaggtcgcat
     6121 tgaacgcctt tgtcggtcca ggtcttcatt tccgccagta gttttttgaa caggttaatg
     6181 ttcaaaccac cgcacaaacc acggtcggtc gacaccacca ggtagcccac gcgtttaacg
     6241 tcgcggtctt ccaggtaagg gtgcttatat tccagattac cgtgtgcaag gtgaccaatc
     6301 actttgcgca tggtttctgc ataaggacgg ctggccgcca tgcgatcctg cgatttacgc
     6361 attttggaag cggcgaccat ctccatcgct ttagtgatct tttgcgtgtt ctggacgctt
     6421 gcgatcttac tacgtatctc ttttgcgccg gccatgagct tctcctcaat gccttgcggc
     6481 ctgccctaag gcaagccgcc agacgttacc aggattgggt tgctttgaag gaatcgagga
     6541 tgcctttcag cttgccttcg atttcgtcgt tgtagccacc ggtctggttg atctcttgca
     6601 tcaacggagc gtgatcacgg tcgacgtaag ccagcagagc ggcttcgaag ctgccaattt
     6661 tcgacagttc aacatccgcc aggtaaccac gttctgctgc gaacagaacc agagactgct
     6721 gcgcaacgga catcggcgca tactgtttct gtttcagcag ttcggtcact ttctgaccgt
     6781 ggtcaagctg cttacgtgtt gcatcgtcaa ggtcggatgc aaactgagag aacgctgcca
     6841 gttcacgata ctgtgccaga gcggtacgga taccaccgga cagttttttc atgatcttgg
     6901 tctgtgctgc accaccaaca cgggatacgg aaatacccgg gttaaccgca ggacgaatac
     6961 cggcgttgaa caggttggtt tccaggaaga tctgaccatc ggtaatggag attacgttgg
     7021 tcggaacgaa cgcagaaacg tcacccgcct gagtttcgat aatcggcagt gcggtcagag
     7081 aaccggtttt ccctttcact tcacctttgg tgaaggcttc aacgtattcg gcgttaacac
     7141 gtgcagcacg ctccagcaga cgagagtgga ggtagaaaac gtcgcccggg aatgcttcac
     7201 gtcctggcgg acgacggagc agcagggaga tctgacggta agcaacagcc tgtttagaca
     7261 ggtcatcgta aatgatcagc gcatcttcac cgcggtcacg gaagtattcg cccattgcgc
     7321 aaccggcata cggtgccagg tattgcagtg cagcggattc agacgcggtt gctaccacaa
     7381 cgatggtgtt agccagtgcg ccgtgctctt ccagtttacg taccacgtta gaaatggtgg
     7441 acgctttctg gccgatagcg acatagatac atttgatacc ggaatcgcgc tggttgatga
     7501 tggcatcgat agccagtgcg gttttacctg tctgacggtc accgatgatc aattcacgct
     7561 gaccacgacc gattgggatc atggagtcaa cggctttata accggtctgt accggctgat
     7621 ctacggactg acgttcgata acgcccggag cgattgcttc tacagcagag aagccgtcgt
     7681 gatccagcgg acctttaccg tcgattggtg cacccagagt gttaaccaca cggcccagca
     7741 ggccacggcc aaccggaact tccaggatac ggccagtaca cttaactttc atgccttcgg
     7801 caaggtcagc gtacggaccc ataacaaccg cacctacaga gtcgcgctca aggttcagtg
     7861 cgatagcgta acggttaccc ggcagggaga tcatttcacc ctgcatacaa tcggccaggc
     7921 cgtgaatgcg gataacaccg tcacttacag aaacaatagt accttcgttg tgagcttcac
     7981 tcacaacatt gaactgagca atgcgctgct tgatcagttc gctgatttcg gtggaattca
     8041 gttgcatgct ccagtcccct taagactgca agacgtctgc aaggcgctca agacgaccgc
     8101 gtacgctgcc atcaatgacc atatcacccg ctcggatgat aacgcctgcc attacagact
     8161 tatcgatttt gcaattcagc ttaactttgc gtgacagacg tttttccatc gcagcagaaa
     8221 ttttcgcgag ctgttgttca ctcagtgcgg cagcggaaat gacgtctacc tcagcggtag
     8281 cctcactcac ggcacgaagg tgaataaact gctccagaac atccgggagc gcgttaagac
     8341 gaccattttc agccattacc cgaatcaggt tctgaccgtt ttcgtccagt tgctcaccac
     8401 aaactgcgat aaacgactcg gcgagcgttt ctggcgcaag cgcgccagag agaagctctg
     8461 ccatttgttc gtttttggtt acctcggcgg caaacgccag catgtcctgc cagcgttcta
     8521 cactttggtg ttcgacggca aagtcaaaag ctgctttggc gtaggggcga gctaccgtaa
     8581 taaattcaga catcagcccc tccctcctta cagttcagcg acaagtttat ccacgatgtc
     8641 gctgttagca gcttcatcca cggaacgttc gatgatcttc tcggcgccag caacagccag
     8701 gatagcaact tgcttacgca gctcttcacg ggcacgttta cgctcggctt caatttctgc
     8761 ctgcgcctgg gccacgattt tagtacgttc ctgttctgcc tcagctttcg cttcgtccag
     8821 aatctgcgag cggcgtttgt tcgcctgctc gatgattacc tgggcttccg ctttcgcttt
     8881 tttcagctgg tcggtcgcgc tggcctttgc aaggtcaagg tccttatgtg ctcgttctgc
     8941 ggaagcaagg ccgtcagcaa tttctttttg acgtttttcg atggctgcca ttaatggcgg
     9001 ccatacgtac ttcatgcaga acagaacgaa caggacaaac gcgatggcct ggccgaggat
     9061 tgttgcgtta agattcacag cacaatgcct ctatttagtt aacgttctga tattgctctt
     9121 taaataaaag caacgcttac tacgcgacag cgaacatcac gtacagaccc agacctacag
     9181 cgatcatcgg gatagcatcc accagaccca taacgataaa gaactgagta cgcagcagag
     9241 gaatcagatc aggttgacgc gctgcgcctt ccaggaattt acccccgagg atgccgatac
     9301 cgatcgcagc accgattgcc gccagaccca tcatcacagc ggcagccatg tacagcagat
     9361 ccatattcag gttttccatg acagtctcca gtttgtttca gttaaaacgt agtagtgttg
     9421 gtaaattaat gttcttcaga cgccatcgac agatagacga tcgtcagaac catgaagatg
     9481 aaggcttgca gcgtaatgat caggatgtgg aaaatggccc acggcacatt caggatccac
     9541 tgtgaccacc acggcaacag accagcaatc agaatgaaaa tcagctcacc ggcatacatg
     9601 ttaccgaaca gtcgcaaacc gagtgaaact ggtttggaca gcaggcttac cccttcaagg
     9661 attaagttga caggaatgaa cgcccagtga ttgaacggct gcagcgtcaa ctctttcgtg
     9721 aagccgccga tgcctttcat tttgatgctg tagaacagaa tcaggataaa tacgcccagt
     9781 gccatagaca gcgttacgtt cacgtccgca gacggaacca cacgcagtgc aggcagaccc
     9841 agtacatgtt cagcaatgta cggcagcagg tcgataggca gtaaatccat caggttcatc
     9901 aggaataccc agacgaagat cgtcagggcc agcggagcaa tcagcttgct tttgccatgg
     9961 tacatgtctt tcacgctacc attaacaaag ccgatcacca gctcaatcgc ggtctgaaac
    10021 ttacctggca caccgctggt cgcctttttg gctacgctac ggaataaaac caggaacaac
    10081 agacccagca ccaccgagaa gaacatggag tcaatattga ttgtccagaa ggtggctggg
    10141 gggttttgtg gatccaccag cgagaatgta cgcaggtcca gctgaaggtt attcaggtgg
    10201 tgtcctatgt aatcctgcgg cgtcatattt tctgaagcca tgatgccttt taccctttgt
    10261 tgttaattac agccggtgcc agtatctgaa ccaccagcac caaaacccac gtaacgatca
    10321 gcggcaagaa taccgccttt aaaaccgcca acgccaccac cagtaacacc aacatcgcca
    10381 gaactttgaa agcttcgcca aatgcgaatg tccaggccac ccggcctttc gctggtgtat
    10441 acgcctggtg acgccaggca aatatcataa acaaaacgtt aggcagaaag actgccaggc
    10501 ccccgcttat tgcagagacg ccccagaagg ggtctttgag gctgaacagc aatccacttg
    10561 ctatcaccac cagtaactga acgagcagaa gcttccgagc aacgtttcga ctcacgagcg
    10621 acacagacat cacgtttttc actcctgctc ccttcgaggt atgccgcgtg tcgtataaaa
    10681 ctttctttaa ggcttagagt caagcatcaa aaagcggtca aattatacgg tgcgcccccg
    10741 tgatttcaaa caataagtag ccaaaaggtg aataaatgtt taaatatttt tccagtgcat
    10801 acaattgcga cttttccgct aaccctgttc gatcatgaaa aactgtaaat aacgcgtaaa
    10861 cactagtgat aaagcgtgct tcagatcaca tattgcgcat gttcgcgcac agtatattta
    10921 tttacttggc aaatgatgcc tttgcaagtt tatgatattt cagtctaaaa acagatactg
    10981 ttttaataaa tgacatttac acaacaaaaa ccacccattg acatttttaa taatgtttta
    11041 acagccaatg atggttctta gcgccgattt ttagcagact gatattttca ctaatgactt
    11101 attttctgct taccaaaaaa agccacgtta tcttgttgat acaaaagagt gaacgtggcg
    11161 ttaaatgtaa ccagttatat cagtagaaaa cttggttgtt gttaacagtc taaccggtca
    11221 attttttatg atttttttga taaaaattaa attttatttg ctttaatcac caccagatga
    11281 cgttcgccat ccagagctgg aacctgaagt ttaaccactg attcgacctg atattcttcg
    11341 ggcaacaaag cgatttcatc ttccggcatt tgccctttca gcgcgtagaa acggccttgc
    11401 tcaccaggaa gatggtggca ccagctcacc atatcgttca gagaggcaaa agcgcggcta
    11461 attacgccat caaatggcgg ctctgaagga aactcttcta ccctgctctg tactggctca
    11521 atattctcca gtttaagctc atgttgcacc tgacgaagga aacgcacgcg tttaccaagg
    11581 ctatccaaca gagtgaaatg ggcttcagga cgcacgatag agagtggaat gcctggcagt
    11641 cctggtccgg tgccgacatc gataaaccgt tcaccttgca gatacggtgc caccacaatg
    11701 ctatcgagaa tatggcgtac cagcatctca ttaggatcgc ggaccgaagt caggttgtac
    11761 gctttgttcc atttatgcag catattcacg taggcaataa gctggttttt ctggtgatcg
    11821 gtaagcgaaa tacctgcgtc tttcagcagt aaggagagtt tgttgagcac ggtgattacc
    11881 tgttcttgat gcgttgcctg gtaagcgggt gcttaccagg catttttaat gcgttatgcg
    11941 ctacgacgca gcataccctg ttttttcagc cacaccagca gaatggagat agccgcaggc
    12001 gtgacgccag aaatacgcga agcctggccg atagaggccg gtttgtgatc gttaagtttg
    12061 gcgatcactt cgttagaaag accggatacc tggcggtaat ccagtgtcgc gggtagcagg
    12121 gtgttctcgt tacgcagctg cttttcgatc tcatcttgct ggcgcgcgat ataaccttcg
    12181 tatttaacct gaatctcaac ctgttccgcc gcctgttcgt ctgtcaacgc aggggcaaac
    12241 ggcgtcagcg tggttaattt ttcataagtc atttccggac gacgcagcag atcttcacca
    12301 ctggcttcac gggaaagcgg cgcagtcagg tgagcattca cttcggctgc agcttccgcc
    12361 gacggggtta cccaggtcga tttcagacgc tgacgctcac gctcgatatt ctcaagtttc
    12421 tcgttaaagc gcgcccaacg ttcgtcatcc accaggccca gttcacgacc gatttcagtc
    12481 aaacgcagat ccgcattatc ttcgcgcagc atcagacgat attctgcgcg cgaagtaaac
    12541 atacgatacg gttctttggt tcctaaagtg cacaggtcat caaccagtac gccgagatac
    12601 gcctgagaac gcgccggagc ccaaccttct ttgtcagcag acagacgggc agcgttaaga
    12661 ccgaccagca aaccttgcgc agcggcttct tcgtaaccgg tagtgccgtt aatctgacca
    12721 gcaaagaaca gcccctggat aaacttgctc tccagcgtcg gtttcaggtc gcgtggatcg
    12781 aagaagtcat actcaatggc ataacccgga cgcacgatct tcgcgttttc catcccctgc
    12841 atggagcgga cgatttgcat ctgcacatcg aacggcaggc tggtggagat accgttcgga
    12901 taaatttcgt tagaggtcag cccttccggt tcaaggaaga tctgatgctg atttctgtcg
    12961 gcgaagcgca tgactttgtc ttcgatcgat gggcagtagc gtgggccgac accttcgatc
    13021 acccctgcgt acattgggct acgatcgagg ttactgcgga tcacatcatg ggttttctcg
    13081 ttggtatgag tgatataaca cggcacctgc tggggatgct gggacgcatt gcccataaac
    13141 gagaataccg gcattgggtt atcgccatgc tgttgcgcca gcacactaaa gtcgatagtt
    13201 cgagcatcaa tacgcggtgg tgtcccggtt ttcagacgac caacgcgcag cggcagttca
    13261 cgcaaacggc gagaaagcgg aatggacggc ggatcaccag cacgaccacc gctgtaatta
    13321 tccagaccga tatgaatttt accgtcgagg aacgtcccaa cggtgagcac gacggctttg
    13381 gcacggaact tcagtcccat ttgggtaaca gcaccgacca cgcgatcgtt ttcgacaata
    13441 agatcttcaa ccgcctgctg gaagatcatc aggttcggtt ggttctccag cgccgtacgt
    13501 accgcctgac ggtagagcac acgatccgcc tgagctcggg tagcgcgaac cgccggtcct
    13561 ttgcttgcgt ttagtatcct aaactggata cccgcttgat cgatcgcttt cgccatcaga
    13621 ccgccgagtg catccacttc ttttaccaga tgtcccttcc caataccgcc gatcgccggg
    13681 ttgcagctca tctgccccag agtgtcgata ttgtgtgtca aaagcagagt ctgttgaccc
    13741 atacgcgccg cggccatcgc ggcctcggtg cctgcatgac ccccgccaat gatgatgacg
    13801 tcaaaaggat ccggataaaa catggtgatt gcctcgcata acgcggtatg aaaatggatt
    13861 gaagcccggg ccgtggattc tactcaactt tgtcggcttg agaaagacct gggatcctgg
    13921 gtattaaaaa gaagatctat ttatttagag atctgttcta ttgtgatctc ttattaggat
    13981 cgcactaccc tgtggataac aaggatccgg cttttaagat caacaacctg gaaaggatca
    14041 ttaactgtga atgatcggtg atcctggacc gtataagctg ggatcagaat gaggggttat
    14101 acacaactca aaaactgaac aaccgttgtt ccttggataa ctaccggttg atccaagctt
    14161 cctgacagag ttatccacag tagatcgcac gatctgtata cttatttgag taaattaatc
    14221 cacgatccca gccattcttc tgccggatct tccggaatgt cgtgatcaag aatgttgatc
    14281 ttcagtgttt cgcctgtctg ttttgcaccg gaatttttga gttctgcctc gagtttatcg
    14341 atagccccac aaaaggtgtc atattcacga ctaccaatac cgattgcgcc aaagcggacc
    14401 gcagaaagat cgggcttctg ttcctgcaat gcttcataga aaggagaaag gttgtccgga
    14461 atatctccgg caccgtgggt ggagctgata atcagccaga tccccgaggc agataaatct
    14521 tccaacagcg gaccgtgcag cgtttcggtg gtaaaacccg cctcttccag cttttcagcc
    14581 aggtgttctg ctacatattc ggcaccgccg agggtgctgc cgctgataag agtgatatct
    14641 gccataaacc gccaccttta ttaagagtgg cgtattgtac gctgtgaacg cgttgggatc
    14701 tacctgtgga aaagtatggg attaaaaaag ccgattatgg cttgatggta cgcatgatcg
    14761 ggttttgcag gacgatcaat gtctcggtgg actgaatttc atcaattgtt tggatcttgt
    14821 tgataagtac atgctggaga gcgtcgatcg aacggcacat tacttttata aagatgctgt
    14881 agtggcctgt tgtgtaatag gcttcagtga cttcagcaag gctttccagc tttgccagcg
    14941 cggaagggta gtctttggcg ctctttaata taatgccgat aaagcagcct acgtcataac
    15001 cgagctgctt cgggctgaca tcaatacgcg ccccggtaat gatccccgcc tgcttcattt
    15061 tctctactcg aacgtgaatc gtccccggac tgacgccaaa ttgtttcgcc agttcggcgt
    15121 aagcggtgcg cgcattgccc attaatgctt ccaggatgcc acggtccaga ttgtcgatca
    15181 gataattttc cataggattt tcttatgcag attgatgatt cattctattt tagccttctt
    15241 ttttaatgaa tcaaaagtgt gttaggcttt ttattgaatg attattgcat gtttgtcggt
    15301 ttttgttgct taatcataag caacaggacg caggagtata aaaaatgaaa accgcttaca
    15361 ttgccaaaca acgccaaatt agcttcgtga aatctcactt ttctcgccaa ctggaagaac
    15421 gtctggggct gatcgaagtc caggcgccga ttcttagccg tgtgggggat ggcacgcagg
    15481 ataacttgtc gggctgtgaa aaagcggtgc aggtaaaagt gaaagctctg cctgatgccc
    15541 agttcgaagt ggttcattca ctggcgaagt ggaaacgtca gaccttaggg caacacgact
    15601 tcagcgcggg cgaagggctg tacacgcaca tgaaagccct tcgccccgat gaagaccgtc
    15661 tttctccgtt gcactcggtc tatgttgacc agtgggactg ggaacgcgta atgggcgacg
    15721 gtgagcgtca attctcgact ctgaaaagca cggtagaggc gatctgggcg ggaattaaag
    15781 caaccgaagc tgcggttagc gaagagtttg gcctggcacc gttcctgccg gatcagatcc
    15841 acttcgtaca cagccaggag ttactgtctc gttatccgga tctcgatgcc aaagggcgtg
    15901 agcgggcgat agcgaaagat cttggcgcgg tattccttgt cgggattggc ggcaagctga
    15961 gcgatggtca tcgccacgac gtgcgcgcac cggattatga tgactggagc accccgtcag
    16021 agctgggtca tgcgggtctg aacggcgata ttctggtgtg gaaccctgta ctggaagatg
    16081 cgtttgagct ttcttccatg gggatccgcg tggatgccga cacgttgaag catcagttgg
    16141 cgctgaccgg tgatgaagat cgtctgcagc tggaatggca tcaggcgctg ctgcgcggtg
    16201 aaatgccgca gaccatcggc ggcggtatcg gccagtctcg tttgaccatg ctgctgctgc
    16261 aactgccgca tatcggccag gttcagtgtg gtgtatggcc agctgctgtt cgcgagaacg
    16321 tcccttctct gctgtaataa tttatcgccg ccagcgtctg agcaggcggc ttcgcatccc
    16381 ggtatcaaag cgccagatat gatcgaaaat gcgcatgatg ccgggtttgc cgtgtgccga
    16441 catcgccacg gcatgaaagc gatgctgatg tacccgctgc agctctttca ctttactcgt
    16501 cacgtcgtca ggcaaccgct gagcgataaa atcagaaatc accaccgcat cggcatcaaa
    16561 ccattccctg ctttgcaagc gttccataat ggcgcgaaaa caactggcaa gatcggtgcc
    16621 gccacgaaac tgctggctta aaaaacggat tgcttgttcg atgccttggg ggcctgaaag
    16681 ctcataacgg acgatctcgg tggaaaatag cataatatag cagcgccggt tttcagcgag
    16741 agcaatgtgc atcaaggcca ggcagaacgc tttcgcacac tgttcattaa agccgcccat
    16801 tgagccggaa gtatccacac agacaataaa cggcccgcgc ggctgttcat cgtaatcttt
    16861 atgtaccacc ggacgttcga tcactttttc acgccacgac tcaccgtgca ggcgataggt
    16921 gagcaactgt ttttccacca gccgacggta aaactcatac tccagttccg ttatccctag
    16981 tgtcgccagt tctggcggca gaagacgtaa aatatcatcg ctttgttgca gaccatcaac
    17041 ctgctcagga accgtcgccg gttcgcgcac catggtgcgg aaggtttcca tctgcgcatc
    17101 gttgcgcggt attgatttgg cttcccgaga acgccccagc tgctctgcca ggcgtttcag
    17161 ttccggctgt tcgttaagaa attcaccgta tttcacaatc aactgatagt cgccacgttt
    17221 aagctgaccg gcgctcatat cccacagacg accagctgca gtattgttat ctgcgagaat
    17281 cggttcaagt tgtccgctca gcgtcatgcg ttcttgaact tcactcaaca gttgttcgcg
    17341 ttcttcttct aatagctgtt gatttaacgt cgttgcttgc acgatcagac ttaaacgcca
    17401 acgctggaga aaaagcgtgt gtaacgctga agttattgtg ctgttagcat caaccaactg
    17461 ccgggcttgt tctgcccatg gggaattcag acgatgcagt aagtccagga tctgtggtag
    17521 ctgcacaata aactgcggcg tggagaggag ctggctttgc tgatagcaca tcacctcttc
    17581 ggtgagttcc ggcgggactc gggcatcttt cagccgactg cgcagcgcct cacgccagcg
    17641 gggaacatca tcagtgattg ccgccttcag gcgtgggaat ttttcaaaga agaccgccag
    17701 ctgcggtgag gccagcagtg cgatgatcat ctcttcgatc aatccctctt cgctgacggc
    17761 cagcatcaca ttaagcgtat ccagcgttag cattgttgtg cctggtgaat ctgtgcaccg
    17821 acatcctgta ggctggcttc aatgcgacct aaccagtcgc cagggataaa cagacatttt
    17881 tgctgttcgc tgaataaagc gtgttgcttg cgccagtcgc tttcaagctc ttccagttgt
    17941 tgttttattt caccaggcag accttcagcc gatgaaccgg gaagtgccag cgtactgcct
    18001 tgtaaactta catcgcgaac gacaagatgt tgggcgctat caacttccag attcagtttc
    18061 tgggcaaagc cgataccgtt cagtttaccg cgaatttcac cacctttgct cagccactgt
    18121 tccagcgcgc tacgctcaaa ggagatatga accacttcca tatcatgcag ttttaacggt
    18181 ttttgcagca gtagagtcag agtggaagca gtaacgttaa caggaagttg atactgctga
    18241 cgacggctga aaatgccgcc cagacgaatt accgttaagg ctgttttatc gctttgttgc
    18301 tgctggagtt gcaggtgacg ttgcacaatc gcgccaaggc gagtcaacat cccttgctgt
    18361 tgccaggcat gaccggtcat caatacatca atttgttgtt gtatcaaatt caggctttgc
    18421 gcgtcatacc acaggcaatc tttcagcaaa atgagatcta ccggggcaac agcactgcga
    18481 ccgctaaaaa aggcgctggc ctgcaataat cggatcgctt ttttccagcg acgatccgag
    18541 acataaggcg catccggtaa tttatcctgt tgctggcgca gcataaaaat cagctcaaat
    18601 acatgatcgg gcagcgtaat ttcaccaatc tctttctgcc agcgttcata ttcttcatct
    18661 gtgacctgca aggcatcagg aaccggattg tcgttttcat cctgttgact ggtcagcatg
    18721 gagcggaaat tcgctttatc ctgcacttta tctaaccaca gacgaatcag catgcggtca
    18781 tataacgctt ccagactgct gtctgcttcc ggcagctcgt tggaggccgc caccagcaga
    18841 cgcatcggga ttttttctac gtgtgcaccg ttgcggaact ggcgctcgtt aatggcggtg
    18901 agcaaggtat taagaattgc cgggcccgct ttccagatct catccagaaa gacaatttct
    18961 gcttccggca ggtaaccgct ggttaaacgt tcatagcgcc cttcatcttt tagcgcctga
    19021 atagaaaggg gaccaaaaac ttcttccggc gtggagaagc gggtcatcag atattcaaac
    19081 gcgcgggcat tctgaaaggc gaattttaag cgccgggcga tcaaactttt ggcaatacct
    19141 ggcgggccaa ggaggaacac actttcacca cttaatgccg ctaataaaca caagcggatg
    19201 gcgtggctac gttcataaag ccccttttcc aacgaactgc tcaggcggga aattctttcc
    19261 gctaataaat gagggtgagc cataatgaag tggcgtcctt tcgtcaaaag ttctgcgtaa
    19321 attgcgagta tagacgtttc ttgctggtgg ctaaaatagt ctcaaagggg gg
//
LOCUS       DAESHI010000079        19267 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.080, whole genome shotgun sequence.
ACCESSION   DAESHI010000079 DAESHI010000000
VERSION     DAESHI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19267
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.080"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..468
                     /locus_tag="KW011_002858"
     CDS             <1..468
                     /locus_tag="KW011_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBH7661145.1"
                     /translation="SGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIET
                     KLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYD
                     PRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            581..1375
                     /gene="panB"
                     /locus_tag="KW011_002859"
     CDS             581..1375
                     /gene="panB"
                     /locus_tag="KW011_002859"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBH7661146.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1387..2238
                     /gene="panC"
                     /locus_tag="KW011_002860"
     CDS             1387..2238
                     /gene="panC"
                     /locus_tag="KW011_002860"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBH7661147.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="KW011_002861"
     CDS             complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="KW011_002861"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HBH7661148.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3700..4080
                     /gene="panD"
                     /locus_tag="KW011_002862"
     CDS             3700..4080
                     /gene="panD"
                     /locus_tag="KW011_002862"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBH7661149.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4084..5313)
                     /locus_tag="KW011_002863"
     CDS             complement(4084..5313)
                     /locus_tag="KW011_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH7661150.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5377..5817)
                     /locus_tag="KW011_002864"
     CDS             complement(5377..5817)
                     /locus_tag="KW011_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH7661151.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5922..6692)
                     /locus_tag="KW011_002865"
     CDS             complement(5922..6692)
                     /locus_tag="KW011_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH7661152.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6689..7615)
                     /locus_tag="KW011_002866"
     CDS             complement(6689..7615)
                     /locus_tag="KW011_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH7661153.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7724..8386
                     /gene="can"
                     /locus_tag="KW011_002867"
     CDS             7724..8386
                     /gene="can"
                     /locus_tag="KW011_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HBH7661154.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="KW011_002868"
     CDS             complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="KW011_002868"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBH7661155.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            9169..11559
                     /gene="gcd"
                     /locus_tag="KW011_002869"
     CDS             9169..11559
                     /gene="gcd"
                     /locus_tag="KW011_002869"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HBH7661156.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="KW011_002870"
     CDS             complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="KW011_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HBH7661157.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEIPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13322..13669
                     /locus_tag="KW011_002871"
     CDS             13322..13669
                     /locus_tag="KW011_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HBH7661158.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13775..14641
                     /gene="speE"
                     /locus_tag="KW011_002872"
     CDS             13775..14641
                     /gene="speE"
                     /locus_tag="KW011_002872"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBH7661159.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14657..15451
                     /gene="speD"
                     /locus_tag="KW011_002873"
     CDS             14657..15451
                     /gene="speD"
                     /locus_tag="KW011_002873"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBH7661160.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15489..15851)
                     /locus_tag="KW011_002874"
     CDS             complement(15489..15851)
                     /locus_tag="KW011_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HBH7661161.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="KW011_002875"
     CDS             complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="KW011_002875"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HBH7661162.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18978..>19267
                     /locus_tag="KW011_002876"
     CDS             18978..>19267
                     /locus_tag="KW011_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HBH7661163.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQV"
BASE COUNT         4757 a         5196 c         4702 g         4612 t
ORIGIN      
        1 agtggaactt ctgtgactgg ttctacggta aaaatgggtg aatatagtgc agaacaaatc
       61 agaaacggtg ccacaccggt tccttttgat atttcacttc aaaactgcgt tcgtgtgact
      121 aatattgaga caaaattagt ttcaacaaag gttggtactg aaaacgggca actccttggt
      181 aatactctca caggtaatga cgcagcaaaa ggagtcggtg tactcataga aggtttagca
      241 actagtaaaa atcctctaat gacattgaaa cctaatgatt caaattctgt ttataaagat
      301 tacgacccga gaggcaaaga cgatacaaca ggaggagttt acccggatca agatacaggt
      361 ataacatacc ctctccattt ccaggccacg ctacaacagg atggaactat accaatagaa
      421 gctggtgagt ttaaagccac cagtactttc caggtaacct acccttaata agtccaccgc
      481 accgccatca tctggcggtg cggtaattga taaatcattg cccccaccaa tgtgactgat
      541 aaaatgccag gcaacacgac atcatttatc aggacacgtt atgaaaccga ccaccattgc
      601 ttcacttcag aaatgtaagc aggataaaaa gcgtttcgcg accatcaccg cttacgacta
      661 cagcttcgcg aaactatttg ctgatgaagg acttaacgtc atgctggtag gcgactcgct
      721 gggcatgacg gttcagggtc acgactccac cctgccggtg accgttgccg atatcgccta
      781 ccacactgcc tccgtacgtc gtggtgcgcc aaactgcctg ctgttggctg acctgccgtt
      841 tatggcgtat gccacgccgg aacaagcctt tgaaaacgcc gcaacggtta tgcgtgccgg
      901 tgccaatatg gtcaaaattg agggcggtga gtggctggtc gaaaccgtaa aaatgctgac
      961 cgaacgtgcc gttcctgtat gtggtcactt aggtttaacc ccacagtcag tgaatatttt
     1021 cggtggctac aaagttcagg ggcgcggcga tgaagcgggc gatcaactgc tcagcgatgc
     1081 attagcctta gaagccgctg gggcacagct gctggtgctg gaatgcgtgc cgattgaact
     1141 ggcaaaacgt attaccgaag cactggcgat cccggttatt ggcattggcg caggcaacgt
     1201 cactgacggg cagatcctcg tgatgcacga cgccttcggc attaccggcg gtcacattcc
     1261 taaattcgct aaaaatttcc tcgccgaaac gggcgacatc cgcgcggctg tgcggcagta
     1321 tatggctgaa gtggagtccg gcgtttatcc gggcgaagaa cacagtttcc attaaggagt
     1381 cacgttgtgt taattatcga aaccctgccg ctgctgcgtc agcaaattcg ccgcctgcgt
     1441 atggaaggca agcgcgtggc gctggtgcct actatgggta atctgcacga tggccatatg
     1501 aagctggtcg acgaagccaa agcccgcgcc gatgtggtcg tcgtcagtat tttcgttaac
     1561 ccgatgcagt tcgaccgccc ggaagatctg gcacgttatc cacggacctt gcaggaggac
     1621 tgcgagaagc taaacaaacg taaagtggat ttagttttcg ccccttcggt aaaagagatc
     1681 tacccgaacg gtactgaaac ccatacttac gttgacgttc ccgggctttc gaccatgctg
     1741 gaaggcgcga gccgtccggg gcattttcgc ggcgtttcga ctatcgtcag caagctgttt
     1801 aacctggtcc agccggacat cgcctgcttc ggtgaaaagg actttcagca actggcgcta
     1861 atccgcaaaa tggttgccga tatgggcttc gatattgaaa ttgtcggcgt cccgattatg
     1921 cgcgccaaag atggccttgc gctcagttcc cgtaatggtt atctgacggc ggaacaacgc
     1981 aaaattgcgc ccggtctgta caaagtttta agttcaattg ccgacaaatt gcaggctggc
     2041 gagcgggatc ttgatgaaat tattgccatt gcagggcaag aactgaatga aaaaggcttc
     2101 cgcgccgatg atattcagat tcgcgatgcc gacacgttgc tggaagtctc tgaaaccagc
     2161 aaacgagctg taattctggt agccgcctgg cttggcgatg cccgcctgat cgacaacaaa
     2221 atcgtcgaac tggcgtaatg cttttactct aaataattca agttgcagga aggcggcaag
     2281 cgagtgacaa attcgtcagg aacgaatttg cccagccgca ggctggcctt cggtgagaga
     2341 catggatgtc tctcattaat ccccaggagc ttacataagt aagtgactgg ggttagcgaa
     2401 cgcagacgca gcacatgcaa tttaaagtat gacgagtata actggcgcta cggctgatgg
     2461 cgccaattat taatttgccc cacgtcatgg cgatggcgtc tgtaagcaat tattcataac
     2521 gcagacaact tagttaatca gcctatcaat ctcagcaaga gataaatttg tcatctctgc
     2581 aacgtcttcc cgagacatcc ctttactcag aaaacgctgg gcgaattctt gcctttcctc
     2641 ctgtcttccc tgctgaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2701 catcatagac tcccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2761 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2821 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2881 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2941 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gtgccagctc
     3001 cggcgagtaa aatatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     3061 ataaaacagt atcggcacca ccagcggcag cttatcgtga tccgcctcca gatgccggtg
     3121 catggcagca atagaataac gcatcatgcg aaagaccatt ttcttatccg gcttgctttg
     3181 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     3241 gtccgtgctg tgtcctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     3301 gttgaggtca caaagttctc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3361 cgtctccgcg tgcattaaaa attgtttaaa cactgcatca tgcggtgtgg tactcggtgc
     3421 atccatcttc cctgccttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgtgc
     3481 catcgtcagc cacctcttta gtgtttaccg agcagcgttc agagaaattt caattggtta
     3541 cagccgttac atggcacgct tcggcacggt acgcaaactg ctgatgtaaa tgccgtcaga
     3601 tgagtagaca ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcat
     3661 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3721 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3781 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat tgatatctgg
     3841 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3901 attatttctg ttaacggtgc ggcggcccac tgcgccagtg tcggcgatat tgtcatcatc
     3961 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat
     4021 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     4081 ttgttatccc tgcggctggt tactcaccag ccgcgacatc gtctccagcg aatccgttct
     4141 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     4201 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     4261 tccgccaaac ggatacgaaa gataccagac atgcggatta aattgcgcca gagcgcggcg
     4321 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4381 atagccatcc acccgatgca aaaaatgggt atgtgactga aaatcaaaaa catcgcgaat
     4441 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg
     4501 acgtttgatg cgcgaggtaa caacaaacgc cgtcgccttc atgccatatt gtttcaatac
     4561 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4621 cgcagggaga ttgatcttat tcttcacgta accttccagc tgcgccatgc tcagtgtcgc
     4681 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4741 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4801 cggaaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4861 acggacctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4921 caaattgtcc gccaataccc caaatggcgc actgcccaca ctcggcgcgt tatagaccgg
     4981 cgtatctttc caggtaacta agttctgatt actcagcggc ttgttgaggt cacccaaacc
     5041 gtcttcaact ttttgtcgcc cctgaaccgg ctcaagatgc cctttatcga taaacccttt
     5101 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     5161 tccggcccga atattcccca cggtcaccat cttgtcgcca atttgcgccc agaccgcagc
     5221 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     5281 catcagcagg agaataacag cttgtttgta catgaaaaaa taaccgaggg tgaaacaacg
     5341 cttttattgt agcaaaagcg ctataagaat cgtgggttaa tactcataca aattatgttt
     5401 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5461 cagttcgaca atacgctcgc gaaactctga actggtcatg gtttcacggc aagccatcat
     5521 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5581 taataatgaa gccacgcggt acggtggcgc tccggctata tcggttaaga agatgacacc
     5641 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5701 taacccacgc cagaaattca cggcccggca ctgaagaagc gccccatgtt ttttctccag
     5761 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5821 cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt
     5881 caaaactcct ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5941 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     6001 ccaaaggtag tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg
     6061 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     6121 agtgagtaaa agaccccacc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     6181 ctgatgtcat cgaacgtttt ggcaaacaca ccgttcagca aacccgcaag ggagaacaac
     6241 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     6301 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6361 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6421 ttacgctgga acttggcacc aaaaaatgat gacgcaacgt tggcgtaggc attggtgatc
     6481 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6541 ccaatacgcg aaccaatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6601 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6661 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6721 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6781 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgcagc acttcaactt
     6841 ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6901 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6961 aggtattttc caccagctca ccgtgttgaa taatgccgat attgcggcac agcatttctg
     7021 cttcttccag gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca
     7081 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     7141 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     7201 cagataacat acgcgcacgt tcgttgcgtt ttccccatag atcgagttgt ttaagatatt
     7261 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     7321 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7381 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7441 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7501 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7561 gaacgccgcc tggataggtt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7621 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7681 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7741 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7801 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7861 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7921 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7981 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     8041 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     8101 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     8161 gtgtgaactg aacgtcatgg aacaggttta taacctaggc cactccacca ttatgcaatc
     8221 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     8281 cttgctgcgt gatctggatg tcacagcgac caaccgcgaa acccttgagc aacgttaccg
     8341 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8401 atgcaacaca tccggcaact tcacacttac tcgtcccgca gaatcacttt gccgatatac
     8461 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8521 atcgagaaac cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc
     8581 agcgtacaaa tcgccagcga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8641 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8701 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8761 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8821 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8881 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8941 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     9001 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     9061 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     9121 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     9181 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     9241 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     9301 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggtgcagtaa acgcgccgcg
     9361 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9421 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9481 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9541 gtggtggcac tgctgattag cggtagtatc ctgacctggg ccggatttaa cgatccgcag
     9601 gaaatcaacg gcaccttaag cgccgatgcc acacctgcgg aagctatctc acccgttgcc
     9661 gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc acctctgaaa
     9721 caaattaacg ccgataacgt ccataatctg aaagaagcct gggtgttccg tactggcgat
     9781 gtgaagcagc cgaacgatcc gggcgaaatc accaatgaag tgacgccgat taaagtgggc
     9841 gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc
     9901 aaagagaaat ggcattacga tcctgagctg aaaactaacg agtctttcca gcacgtaacc
     9961 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
    10021 gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gactgattgc gattaacgct
    10081 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
    10141 atgccagaca ccaaaccggg tctgtatgaa ccgacttcgc caccgattat caccgataaa
    10201 accatcgtga tggccggttc agttaccgat aacttctcaa cccgcgaaac gtctggcgtg
    10261 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc cggcgcgaaa
    10321 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc aaactcctgg
    10381 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10441 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10501 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10561 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10621 gtgccagtta tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgcaat
    10681 ggcgaactgg tggttccggc cccggaaaaa ccggtgccgc aaggtgctgc gaaaggcgat
    10741 tacgttactc cgacccagcc gttctctgaa ctgagcttcc gtccgaagaa agatttgagc
    10801 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10861 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggcacgct ggtcttcccg
    10921 ggtaacctgg gaatgtttga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10981 attgccaacc caatggcact gccgtttgtt tcgaaactga tcccacgcgg tccgggtaac
    11041 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    11101 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccgtttgg tctgccgtgt
    11161 aaacagccag catggggtta tatctcggcg ctggatctga aaatcaatga agtggtgtgg
    11221 aagaaacgta ttggtacgcc gcaagacagt atgccgttcc cgatgcctgt accggtgccg
    11281 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    11341 atcgctgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11401 tggcagggcc gtttaccagc gggtggtcag gcgacgccaa tgacctatga agtgaatggc
    11461 aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11521 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagagatcc
    11581 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11641 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11701 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11761 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcatg ccgtgaatat
    11821 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11881 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11941 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccgtgc cccatatggc
    12001 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    12061 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    12121 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    12181 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggcgcaatcg
    12241 ccatccccat ctggctgacc ggtagcgtca ccagatcaaa gggtttgttg tcgttaacct
    12301 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12361 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12421 aattgagcga gcgggcgtta cagccattga gcaaacgcag gcgcagccag ccacgcgggg
    12481 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12541 cggcggtcat cacatccagt tgataatcaa tctgcccatc agcgttaaat tttttatcct
    12601 gaacaatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12661 cgtcatcttc aattaccacc agcccagcca accccatcgc cacctgtcgc ccggtcttgc
    12721 catgttgatg tggatggaac cagcaggtag cggcaggttg atcaacgttc aacgtcaccg
    12781 tacgtttgcc gcctggcgga ataattccct gcgggccgcc gtcgacgtca cccggtattt
    12841 ccagcccgtg ccagtgcaac gtcgtctctt ccgccagttg gttatagata tcaaccgtta
    12901 ccgctttgcc gcgctgtaat ttcaccgccg gccccagcag attgccgtta tagccccagg
    12961 tagttgcagt tttctcgcca aaggtagact gacctgcgcc aatagttaac tgaatgcgat
    13021 tacgggcatc ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga
    13081 ctgcgcggct ccacagcggc aaggctgaag ccacacccag cgcgacggaa tattttaaga
    13141 aatcacgacg ttgcatagtt atttccttat tcttaagcag gcgaaacaaa atcaaacgtt
    13201 gagcatagtc cttcccctta cgggaaggtc aagccgcaga cacaatttaa tcgacaataa
    13261 tggtcgtcgc ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc tggtagaagc
    13321 aatgaagacg tttttcagaa cagtgttatt cggcagcctg atggccgtct gcgcaaacag
    13381 ttacgcgctc agcgagtctg aagccgaaga tatggccgat ttaacggcag tttttgtctt
    13441 tctgaagaac gattgtggtt accagaactt acctaacggg caaattcgtc gcgcactggt
    13501 ctttttcgcc cagcaaaacc agtgggatct cagtaattac gacaccttcg acatgaaagc
    13561 cctcggtgaa gacagctacc gcgatctcag cggcattggc attcccgtcg ctaaaaagtg
    13621 caaagccctg gcccgcgatt ccttaagcct gcttgcctac gtcaaataat ccctgatacc
    13681 tttttgtaga aatattgacc gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt
    13741 tttttacggg tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac agtggcatga
    13801 aacgctacac gaccagtttg ggcagtactt tgcggtagat aacgttctgt atcatgaaaa
    13861 gaccgatcac caagatctga tcatttttga gaacgctgca tttggtcgcg taatggcgct
    13921 ggatggcgta gtacaaacca ccgagcgcga cgagtttatc tatcatgaga tgatgaccca
    13981 tgttccgcta ctggcccacg gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg
    14041 tgccatgctg cgtgaagtaa cccgacataa aaacgttgag tcaatcacga tggtggaaat
    14101 cgatgcgggt atcgtgtcgt tctgccgtca gtatctgccc aaccataacg ccggtagcta
    14161 cgacgatccg cgctttaagc tggtgatcga cgatggcgtc aatttcgtta atcaaaccag
    14221 ccagaccttt gatgtcatta tctccgactg caccgatcct atcggtcccg gcgaaagtct
    14281 gttcacctca gcattttatg aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt
    14341 cgcacaaaac ggcgtctgct ttttacagca ggaagaagcc atcgacagct atcgcaaact
    14401 cagccattac ttcagcgacg ttggctttta tcaggcggcg atcccgacct attacggcgg
    14461 tatcatgact tttgcatggg cgacagataa cgacgcctta cgccatctct caaccgaaat
    14521 tattcaggcg cgttttctcg cttctggcct gaaatgccgt tattacaatc cggcaatcca
    14581 tacggcagct tttgccttac ctcagtatct gcaagacgca ctggcttcac agccgtccta
    14641 aggagaagat aagaaattga aaaaactgaa actgcatggc tttaataatc tgaccaaaag
    14701 tctgagtttt tgtatttacg atatctgcta cgccaaaact gccgaagagc gcgacggtta
    14761 tattgcttat atcgatgaac tctataatgc caaccgtctg accgaaatcc tgtcagaaac
    14821 ctgttccatt atcggggcca atattcttaa catcgcccgc caggattacg aaccacaggg
    14881 tgccagcgtc actattctgg tgagtgaaga accggttgac ccgaaactca tcgacaaaac
    14941 agaacacccc ggcccactgc cagaaacggt cgttgcccat ctcgataaaa gtcatatttg
    15001 cgtacatacc tacccggaaa gtcatcctga aggcggttta tgtaccttcc gcgccgatat
    15061 tgaagtctct acctgcggcg tgatttctcc gctgaaggcg ctgaattacc tgatccacca
    15121 gcttgagtcc gatatcgtaa ccattgatta tcgcgtgcgc ggttttaccc gcgacattaa
    15181 cggtatgaag cactttatcg accatgagat taattcgatt cagaacttta tgtctgacga
    15241 tatgaaggcg ctgtatgaca tggtggatgt gaacgtctat caggaaaata tcttccatac
    15301 caagatgttg cttaaagagt tcgaccttaa gcactacatg ttccacacca aaccggaaga
    15361 tttaaccgac agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat
    15421 ttattacggg cgcaatatgc cagctgttta acggctctgg cggagctccc aggctccgcc
    15481 agatttattt acttctgctg cacgaaattg cggtaagccg ccacgacctg cagaaaatcc
    15541 tcaacgccgc atagcgacag gctttcttcg tcgtagtagt tcatcccttc ttccatttca
    15601 tcgccagcga attccagttg attggcgcga accatcacct cttcaccgtc catccacagg
    15661 gtgtattcat gccctgcccg ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct
    15721 tgttccactt catcaagcaa agccaggttt tctttcacct cttcattaaa ccagtgcccg
    15781 accacttcat gccccatgga catacgcacc tttaccactc cggtaatatc gcgcagaaat
    15841 tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca ttgcgctatt
    15901 gatgtaatta ttgcagcctt accggagaaa aacagcggaa cggcgatggt ttagaaataa
    15961 aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt
    16021 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    16081 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    16141 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    16201 gcagaagcca ggaagacatt cgcgccagtc cccagacggt tcgggaagtt acgggtagag
    16261 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagagaacag
    16321 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttcagtc
    16381 aactgtgcgg cgtccatgcg ggttggcggt gccacccaca ggcgggtcgg caactggcct
    16441 ttgtgcgcat ccagcagttt acccgcagca cggaagtgac cgatgttagt catgcaagaa
    16501 ccgataaaca cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcgtcc
    16561 gggtcgttcg gtgcacagag gattggctct ttaatatccg ccagatcgat gtcgatcact
    16621 gccgcgtatt cagcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16681 atgccctgaa tacgacgttc gagggtacga cgatcgccat aaccttccgc gatcatccac
    16741 ttcagcagga cgatgttaga gttcaggtat tcgatgatcg gttctttgtt cagcttgata
    16801 gtacaaccag cggcagaacg ctcagcggac gcatcggtca gttcaaatgc ctgctcaact
    16861 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16921 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcgggatcgc gtgtaccaga
    16981 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    17041 atatccagcg gcattacgcc tgttgcggct gcaaatgcca ccagaccaga acccgccggg
    17101 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    17161 agcagcatac ggttcagcca ggagtggatt acgccgtcac ccggacgcag cgacacacca
    17221 ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    17281 tacgccgcgg tatggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    17341 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgtgaacc tacagaagtc
    17401 attttcggtt cgcagtacgc gcccggacga atacctttca caccgcaggc acgacctacc
    17461 attttctgag ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17521 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17581 atcagcggaa tacggccacc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17641 aaggtcgcca gcagttcgcc ggtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17701 tcaatcacgt cgcccatgtt caggttagag acgtcgactt cgattggcag tgcacccgcg
    17761 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17821 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17881 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17941 tgttgcagag cttcgatttg cttgatcggg ccaacaacac ctggctggtc tggctcaata
    18001 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgcgaccac
    18061 gcatccggtg ccggagaaag gtcatcagtg ttggtttcgc cagtgacttt gaagacggta
    18121 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    18181 tgcattacct gcttcgcata ttcgttgcct gctttggctt tctcttctac gtcatagaag
    18241 ttatcgaaca tcagcagtgt gtgagaaagt gctttggcag cgatcggtgc cagtttggca
    18301 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt acccagcagt
    18361 tcgatggctt tttccggagt cagcagaggg gatttggctt cgcctttagc gatagcagcc
    18421 aggaagcctg ctttgacata ggcggcttca tcgacgccag ggggaacacg gttggttaat
    18481 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagtgcg
    18541 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18601 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18661 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18721 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18781 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18841 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18901 cgcctcttta tactcggatt aacagcacct gcgggtggca gttcgccgac cattgcgatt
    18961 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcactaa
    19021 tttgcagtgc cgggctttgt gccgcctctg ccgggctata tataaaaagc cgcacagtgg
    19081 aagcgcctgt ggaaacgcaa tcgacacaac agactgcgcc tgacatcacc gcagttacgc
    19141 ttcctgcaac ggtttccgca ccgcccgtaa cgcccgccgt cgtcaaatcc gcattcagca
    19201 ctgcacaaat cgatcaatgg gttgcgcctg tcgcgctgta ccccgattct ctgctttcac
    19261 aagtgtt
//
LOCUS       DAESHI010000080        18841 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.081, whole genome shotgun sequence.
ACCESSION   DAESHI010000080 DAESHI010000000
VERSION     DAESHI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18841
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.081"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(250..2121)
                     /gene="ppiD"
                     /locus_tag="KW011_002877"
     CDS             complement(250..2121)
                     /gene="ppiD"
                     /locus_tag="KW011_002877"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBH7661164.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2313..2585)
                     /gene="hupB"
                     /locus_tag="KW011_002878"
     CDS             complement(2313..2585)
                     /gene="hupB"
                     /locus_tag="KW011_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HBH7661165.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2794..5148)
                     /gene="lon"
                     /locus_tag="KW011_002879"
     CDS             complement(2794..5148)
                     /gene="lon"
                     /locus_tag="KW011_002879"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBH7661166.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5336..6610)
                     /gene="clpX"
                     /locus_tag="KW011_002880"
     CDS             complement(5336..6610)
                     /gene="clpX"
                     /locus_tag="KW011_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HBH7661167.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6736..7359)
                     /gene="clpP"
                     /locus_tag="KW011_002881"
     CDS             complement(6736..7359)
                     /gene="clpP"
                     /locus_tag="KW011_002881"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBH7661168.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7605..8903)
                     /gene="tig"
                     /locus_tag="KW011_002882"
     CDS             complement(7605..8903)
                     /gene="tig"
                     /locus_tag="KW011_002882"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBH7661169.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKRALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9247..9564)
                     /gene="bolA"
                     /locus_tag="KW011_002883"
     CDS             complement(9247..9564)
                     /gene="bolA"
                     /locus_tag="KW011_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HBH7661170.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9869..10447
                     /locus_tag="KW011_002884"
     CDS             9869..10447
                     /locus_tag="KW011_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBH7661171.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10491..11966
                     /gene="ampG"
                     /locus_tag="KW011_002885"
     CDS             10491..11966
                     /gene="ampG"
                     /locus_tag="KW011_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HBH7661172.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            12428..13375
                     /gene="cyoA"
                     /locus_tag="KW011_002886"
     CDS             12428..13375
                     /gene="cyoA"
                     /locus_tag="KW011_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HBH7661173.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            13397..15388
                     /gene="cyoB"
                     /locus_tag="KW011_002887"
     CDS             13397..15388
                     /gene="cyoB"
                     /locus_tag="KW011_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HBH7661174.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            15378..15992
                     /locus_tag="KW011_002888"
     CDS             15378..15992
                     /locus_tag="KW011_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HBH7661175.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            15992..16321
                     /locus_tag="KW011_002889"
     CDS             15992..16321
                     /locus_tag="KW011_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HBH7661176.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            16333..17223
                     /gene="cyoE"
                     /locus_tag="KW011_002890"
     CDS             16333..17223
                     /gene="cyoE"
                     /locus_tag="KW011_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HBH7661177.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            17408..17719
                     /locus_tag="KW011_002891"
     CDS             17408..17719
                     /locus_tag="KW011_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBH7661178.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            17703..18245
                     /locus_tag="KW011_002892"
     CDS             17703..18245
                     /locus_tag="KW011_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH7661179.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNSVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(18301..>18841)
                     /locus_tag="KW011_002893"
     CDS             complement(18301..>18841)
                     /locus_tag="KW011_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HBH7661180.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
BASE COUNT         4214 a         4955 c         4580 g         5092 t
ORIGIN      
        1 cgtcggtttc gccacagaag ctgccgccag cgcgctatga gacattccgg cacaggccag
       61 ggacagggta atgagcagtg ctttaattcc gtgtttcata ctgttttctc cttgtttgtt
      121 aacagtggaa tcaccttaac gggagtgaaa acagtaaaca aatggcaatg ttcagaaatg
      181 gaaaaggccg cgaaagcgac cttttcttgt tacagctgca acgcattttt ttgcgaggcg
      241 gcttcaggat tattgctgtt ccagcgcatc gccaattttg attttcgcct ctttacgcag
      301 gttactcatc agagcttcaa agacgatttg tgcgttgttc tgggtgatac cctgcaccat
      361 cgcttttttc tgatcttccg gcattgaacc ttgtttcact tcatccagcg ccagcagaac
      421 cacattacct tgcatatcgg tcgccatacc gtagctcggt ttgtctttcg ctggcagtgg
      481 cagtgcaaac gccgcctggc taatcgggtc acgaccggaa cggcttaagg ttttcggctc
      541 gccaaatttc agaccggcag cctgcatagc ttccgcccct ttgccggctt tcaaatcaac
      601 cagcagtttc tcagcatcca ctttcgcctg ttgttcagct ttgttgtgct gaaccagtgc
      661 cttaacttgt tcctgaacat ctgccaacgg tttcaccgct tccggtttgt gctcgctgat
      721 gcgcagcacg aatgcgcggt cgccgtctac ggtgatgatg tcagagttga tgcccggcgc
      781 gccgttttca cctaccagac cgccgttaaa gatagcgtcg gcaaccggct tgaagttcaa
      841 ctcttccggc aggttatctt tgctgaacca acccgtctga gtggctttaa cgccggcagc
      901 ttgctctgca ccggccagag actcggtgtc gttgcttgcc gcatcgctca ctttctgctg
      961 cagcgcatag tacgcatcga gggctttttc gtgtttcact ttcgccgcaa tgtcgtcacg
     1021 tacttcgtct aacgatttca ctttcgctgg ctgaatgtcg tccagacgta caatcaggaa
     1081 accgaccgaa gatttgatga caccagacag ttggcctttt tctttcagac cagcattttt
     1141 cagttcgtcg gggatagtgg catcttctaa ccaacccata tcgccgccgt tacgggcaga
     1201 gataatatcg gcagattttt ctttggctaa tgcagcaaaa tcaccgcctt tattcagctc
     1261 atcaagtacc gctttcgctt catcttcagt tttggtctgg atgatgctgt agcgggtacg
     1321 ctgcggctgg gtgaattgat cctgatgctg atcgtagtag ctctggatat ccgcatcgct
     1381 aaccggttgc tgcatcgttg cggcatccag cttgatgtaa ctcacgcgga attgttccgg
     1441 cgtcatgaaa ttgtttttgt tttgttcgta gtagctggca atctcctgtt cggtcacagg
     1501 ctgcttcgcc gccagcgcgt taacatcgat agtcgcctca cgcaccacgc gttgttgcgc
     1561 gaccagtgcc gccagctcgt cggtttcacc tttcagcata aaatcggtac cggcaacacc
     1621 gttaatcagc tgttgggtag tgagctggtt acgcagcgcc tgggcgtact gatcggcggt
     1681 catccccatc tggttgagga taccgttata gcggctgtta tcaaatttgc cgtcaacctg
     1741 gaaggctggg gtcgcgaaaa tcgcctgttt aacctgctca tcactgatac ccagtttcag
     1801 ctcacgagcg tactgatcca acagcgcctc gtcgatcaga cgattcagca cctgttgacg
     1861 cagggttttc atatagcctt cgttcgctgc cagctcggag tattgatcgc ccagctgttg
     1921 ctgcatgcga ttacgctcgc tgttaaaggc gttctcgaac tgcccacggc tgatttcctg
     1981 gtcattcact tttgcggcgt agttattgcc tccgccaatc aggtaaccac tcacgccggt
     2041 caatatgaac gacacgataa tgataccgaa aataatcttg agcacgagac tgtttgcagc
     2101 cgtgcgtaag ctgtccatca tggtgtaaca acactccgct gtagatgact gtgtacctca
     2161 cgcaacgtat cgatgcgcgc aaaggcctat tgtgacaaga aacgggggca attgtcagcc
     2221 cacaagacac agaaagtcac cgaatacaaa taaaaaaggc acatcagtag atgcgccctt
     2281 gaacttcgtc acatccccac tggggacaac gcttagttta ccgcgtcttt cagtgcttta
     2341 cctgcacgga agctcggtac tttagcggca gcgatggtga tctctttacc ggtctgcggg
     2401 ttgcggccag tacgggcagc acgctcttta acggcaaaag taccaaaacc taccagtgct
     2461 acatcatccc cttctttcag agattcagtt acggaagcaa taatagcatc taacgcacgg
     2521 ccagccgcag ctttagagat atcagcccct gcagcaatct tgtcgatcaa ttgagattta
     2581 ttcactcttc tcttcctctt tataatttat atcgcacttg aatccttcaa ggtacgaacg
     2641 cgcagcagtt atatcaggcc agccatcccc ttacaacacc ccttaataag ggcaagcccg
     2701 atccgccatc taacttagcg agacaaaaaa aggctggcaa gcccgaatta gcctgccagc
     2761 cctgttttta ttagtgcatt ttgcgcgagg tcactatttt gcagtcacaa cctgcatgcc
     2821 agacggttca ttttgcagcg ccagagtcag aacttcctca atgcgcttca caggatgaat
     2881 gtccagatcg gcaattacgt tgtcaggaat ctcttccaga tcgcgtttat tttcgaacgg
     2941 aattagcact gttttaatcc cgccgcgatg cgctgccagg agtttttctt tcaaaccacc
     3001 gatcggcagt acctgaccac gcagagtgat ctcaccggtc attgccacat cggcacgaac
     3061 cgggttaccg gtcaggcaag aaaccagcgc ggtgcacata gcaataccgg cactcggacc
     3121 atctttcggc gtcgcacctt ccggtacgtg gacgtggatg tcacgttttt cgtaaaaatc
     3181 agggttgatc cccagttttt ccgcacgcgc acgaaccacc gttaacgccg cctgaataga
     3241 ctcctgcatc acttcgccga gcgaaccggt ataggtcagt ttgcctttac ccggaacaca
     3301 tgcggtttca atggtcagca agtcaccgcc cacttccgtc cacgccagac cggttacctg
     3361 accgacacgg ttttcgttat cagcgcgacc atagtcgaaa cgctgaacac cgaggtagtc
     3421 atgcaggtta tcgccgttaa tttcgatatg ttttaatgac ttatcgagca gtaactgctt
     3481 aaccgctttg cggcacagtt tggagatttc acgctccaga ccacgcacgc ccgcctcacg
     3541 ggtgtagtaa cgaataatgc cgataatggc gctatcgtcg acggtcagct cacctttttt
     3601 cagtgcatta cgttcaatct gcttcggcag caggtgacgt ttggcgatgt tcagtttttc
     3661 atcttcggta taaccggaga ggcgaatcac ttccatacga tccagcagcg gtgccggaat
     3721 gttcatggag ttcgacgtcg cgacaaacat cacgtcgctg agatcgtaat ccacttccag
     3781 gtagtggtcg ctgaacgcta cgttctgctc tggatccagc acttcaagca gtgcagaggc
     3841 cggatcgcct cgcatgtcag aagacatttt gtcgatctca tcgagcagga acagcgggtt
     3901 tttcacgccc actttcgcca ttttctggat cagtttaccc ggcatagaac cgatgtaagt
     3961 acggcggtga ccacggattt ccgcttcatc acgcacgccg cccagcgcca tacggacata
     4021 tttacgcccg gtggctttgg caatggactg accaagagag gttttaccta cccccggcgg
     4081 ccctaccagg cagaggatcg gtcccttgat tttgttgaca cggctttgaa ccgcaaggta
     4141 ctcaaggatg cgatctttca cgcgctccag accataatgg tcggtatcaa ggatttcctg
     4201 cgcctgacgc aggtcttttt tgaccttgct gcgcgcattc cacggtacct gtaccatcca
     4261 gtcgatataa ccacgcacta cggtcgcttc tgccgacatc ggagacatca ttttcagctt
     4321 ctgcaactct gcttccgctt tctcttttgc ctctttcggc atcttcgccg cgtcgatttt
     4381 gcgcttcagg gcttcgtttt cgtccggcgc gtcgtccatt tcaccgagtt ctttctgaat
     4441 agctttcatt tgctcgttca gatagtactc acgctgggat ttctccatct gctttttaac
     4501 gcggttgcga atgcgtttct caacctgcag cagatcgatt tccgattcca tcattgccat
     4561 cagatattcc agacgttcgt taacgtcgga catctccaga acggactgtt tgtcagccag
     4621 tttcagcggc atatgtgcag caatggtatc cgccagacgc gccggatcgt cgatgctatt
     4681 cagcgacgtc agcacttctg gtgggatttt tttgttcagc ttgatgtagc cttcgaactg
     4741 gctgattgca gtacgcacca gcacttcctg ttcccgctca tcaatggtcg gcgactccag
     4801 atactccgcc ttcgcagaaa agtgttcgcc attgtcagag agcgcagaaa tacgcgcgcg
     4861 ctgtaacccc tcgaccagca ctttgacggt gccgtcaggc agtttcagca tctgcaatat
     4921 agaggccacg gtcccgacgg tgaaaagatc gtttacaccc ggctcatccg ttgaagcttc
     4981 tttctgcgcg accagcataa tttttttatc atggtccatc gccgcttcca gacaacggat
     5041 agatttttcc cgcccgacaa ataaggggat gaccatgtgc ggataaacca ccacatcgcg
     5101 cagcggcaat acggggattt caatgcgttc agaacgctca ggattcatag agctctctct
     5161 tagtttaatt tccgccaggt aatcagatga cacgactgtg cttcacgcca tctattaaca
     5221 tgtacgtcag tatatgggga tgtttccccc acattcaacg ccgagaatag aggaaaaatt
     5281 aaaggggaga taaaatcccc cctttttggt taactaattg tatgggaatg gttaattatt
     5341 caccagatgc ctgttgcgct tccggcttac cataaatcag caacggtttg ctttgaccat
     5401 caattaccga ctcgtcgata accacttttt cgacgtcttc catggacggc agatcgtaca
     5461 tggtatcgag cagtgcggct tctacgatgg aacgcaggcc acgggcaccg gttttacgcg
     5521 ccatcgcttt cttagcgata gcatccagcg cctcgtcacg gaattccaga tccacgcctt
     5581 ccagattaaa cagcgcctga tactgcttgg tcagggcgtt tttcggctct ttgaggatct
     5641 gaatcagagc ttcttcgctc agttcattca acgttgctac aaccggcaga cgaccaatga
     5701 actcagggat aagaccaaac ttgatcagat cttccggttc aacctgcgcc agcagctcgc
     5761 cttcgcttgc tttgtcggac ttcgctttta ccgtcgcgcc aaaaccaatg ccggagccgg
     5821 tttctacacg gtgggaaatc actttatcca gaccggcaaa cgcaccgcca caaataaaca
     5881 ggatcttaga ggtatcaacc tgcaagaatt cctgctgcgg atgtttacgc ccaccttgcg
     5941 gtggaacagc agctaccgta ccttcgatca gtttcaacag tgcctgctgt acgccttcac
     6001 cggaaacgtc tcgggtaatg gacgggttgt ctgacttacg agaaatcttg tcgatttcat
     6061 cgatgtagac aatcccgcgc tgcgctttct gtacgtcgta atcgcacttt tgcaacagct
     6121 tctgaatgat gttttcaacg tcttcgccca cataaccggc ttcggtcagc gtggttgcgt
     6181 cggccatggt gaacgggacg tccagcaggc gcgccagcgt ttcagccagc agcgttttac
     6241 cggaaccggt cggaccgatc agcagaatgt tacttttgcc caactcgaca ccattgctgg
     6301 tatcgccgtt gcgcagacgt ttgtaatggt tgtataccgc gaccgccagc acttttttcg
     6361 cctgttcctg gccgataacg taatcgtcca ggtggttgcg aatttcatgc ggcgtcggta
     6421 gcgcactgcg ttcacgatgc ggtgcaactt ctttaatctc ttcgcgaatg atgtcgttac
     6481 ataaatcaac acattcgtcg cagatataca cggatggacc ggcaatcagc ttgcgcactt
     6541 catgctggct tttgccgcaa aaagagcaat acagcaattt gcctgagcca tctttgcgtt
     6601 tatctgtcat gggtcaaaac ctcttctttg ttctttgtgc cgcacacgac gacgcaaatg
     6661 ccattctcag gcgcaagccg cttacagcgt tgtgccgccc tggataagta tagcggcaca
     6721 gttgcgcctc tggcatcaat tacgatgggt cagaatcgaa tcgaccagac cgtattccac
     6781 cgcttcaggg gcggaaagga agcgatcgcg ctcggtatca cgttcaatct gttctaatga
     6841 ttgacccgta tgaagcgcca taagttcatt catgcgccct ttaactttca gaatttcacg
     6901 ggcatgaatt tcgatatcgg tcgcctggcc ctggtagccg cccaacggtt ggtgaatcat
     6961 cacgcgcgaa ttcggcaggc aaaaacgttt accttttgcc cctgcggtca gcaagaaagc
     7021 gcccatcgag gccgcctggc ccatacagat ggtgctgaca tcaggcttga taaactgcat
     7081 ggtgtcatag atagacatcc cggcagtgat caccccgcct ggggagttaa tgtacagata
     7141 gatatctttt tctgggtttt ccgcttctag gaacagcatc tgcgccacaa tcaggttcgc
     7201 catgtggtct tcaacctggc cagtcagaaa aatgacgcgt tccttaagta gacgagaata
     7261 gatatcaaaa gagcgctcac cgcgtgaggt ctgttcaatg accatcggca ccagcgccat
     7321 atggggtgca aagttatctc gttcgccgct gtatgacatt tccgtctcct ggataaaatt
     7381 gaaaaaacct gctgtaccga ttgtaacctt atggacggat tatcagctag tccctgtgtt
     7441 atgggtacgg catcaccata tttcaagcat aacaatcttt tgttgttacg ctaacaccga
     7501 aagaggcttt tcgcacggtt ccatgcaaaa ttcatgacaa aaaaaagccc accacctggc
     7561 ggtgacgggc ctttgtgcga atttagcgcg ttatgctgcg taaattacgc ctgctggttc
     7621 atcagctcgt tgaaagtggt ttctttttca gtcactttcg ctttcgccag tacagcttca
     7681 acagcctgtt cttccagagc aacattgcgc atgttgtcca tcagttcttt gtttttgctg
     7741 tagaactcga taacttcttt cggatcttcg tacgcagaag ccatctcttc gatcaggcct
     7801 ttcacgcgct cttcgtcagc tttcagctcg ttggtgcgga taacttcgcc cagcagcagg
     7861 ccaacaacta cgcggcgttt agcctgttct tcgaacagtt cgcgcggcag ttccagagct
     7921 cgtttttcgt tgccaccgaa acgctgtgca gcctggcgac gcagaacgtc gatttcgctg
     7981 tcgatcagcg cagccggtac gtcgatgtcg ttagctttta ccagaccttc gatcgcctga
     8041 gacttaacgc ggttacggat ggcgctcttc agctcgcgct ccatgttttt acgcacttca
     8101 gcgcgcagac cttctacgga accatcttca acgccgaaac gtttgatgaa ttctgcagtc
     8161 agttccggca gttcacgctc ttcaactttc ttcaggttga tagcgaattt cgctgcttta
     8221 cctttcagat tttctgcgtg gtattcttcc gggaaggtca cgtcgatggt gaactcttcg
     8281 ccagctttgt ggcctttgat accgtcttca aagcccggga tcatacgacc ctggcccatc
     8341 gccagtacga aatcagacgc tttaccgcct tcgaactctt cgccgtctac agaaccggtg
     8401 aagtcgatgg ttacgcggtc ttctgcttca acggcgccgt ctttttcttt ccaggtcgcc
     8461 tgctgtttac gcagagtatc cagcatgccg tcaacgtcag cgtcggtcac ttcaacgatc
     8521 ggtttttcaa cttcgatcgc ttccagaccc tgcagttcaa cttccggata aacttcaaac
     8581 tctacagagt aggtgaagtc ttcacccagc ttgtattcgc ccggaacata agtcggtgcg
     8641 ccagccggat tgattttttc tttaatgatg gcgtcaatga agttacggct catcaggtca
     8701 cccagaacgt cctggcgtac agacgcgcca taacgctgag caacgatatt cattggcact
     8761 ttgcctttgc ggaagccgtc aatacgtact tttttcgcaa cgttgaccag ctcgctttta
     8821 acagcggtct cgatgctgtc agcagcgata gtaatcgtta cacggcggcc aaggccttga
     8881 gtggtttcaa ctgaaacttg catcttgtta cctcaaaaaa tcacagtgct cggtcaactc
     8941 ttaccccgca agcacaggtt gttggcttcg cggaaccggg atgttcttat aatcaatcac
     9001 attccctgtt accagaatca tcccgaaagc attcaaatag gacgcggcat tatagcggca
     9061 tcacttttat gagtcgagaa cggttattgc gcgctgtcgc gtattttttc acaattcccg
     9121 ggcaattttc gtctaaatgc tgggcaaaaa gcgcaaaatc cagacaaaac aaaacggccc
     9181 gcaggccgtt tttcataaaa tctttagcaa catactggaa aagcgccgac agttgcaaat
     9241 gcgtttttac gcgatgcttc ctgctccacg acagggagga gaggcaaaga cggtgtcctg
     9301 caacccttcc cactccttaa tagtgtaagt atgcagagcc agcgcatgaa cggtagtaga
     9361 gagttcctcc gctaaagtac tgtaaatcat tcgatgacga ttcagaaaac gttcacccgt
     9421 aaaacgatcg ctgaccagca caactttaaa atggctttca gagccggctg ggacattgtg
     9481 acgatagctt tcatccacta cttcgaggaa tacgggttgg aacgccgccc ttaatttttc
     9541 ttctatccgc tcacgtatca tcatgaaata tctcctccga caacgctgag atgtcatcca
     9601 tccctttaaa tactagccgc ttttaccgtt tccattgcag cttaacaaca aatatttagg
     9661 tttcgtcctg gtttttccgt caagcgtatg aagtttactg atttaaagat tgaaacacag
     9721 gcctcgcatc agcgtttctt ttaccagggg tgattatttc gccaacatga tgaatttaca
     9781 tgccttaccc acttcccctc gccgttggca atgttatgat ggcgggaatt tttaccaaac
     9841 acgcttaaga gtctctgaga gcccgaacat gttcaaaaaa atcctcttcc cgttagttgc
     9901 tctgtttatg cttgcaggat gcgcaaaacc gccaacaact attgaagttt ccccgacgat
     9961 tacactgcca cagcaggatc caagcctgat gggcgtcacc gtaagcatta atggtgccga
    10021 tcagcgtacc gatcaggcgc tggcaaaagt cacccgcgat aatcaaatcg ttaccctgac
    10081 cgcctcccga gatctgcgtt tcctgctgca agaagtgctg gaaaaacaga tgaccgcgcg
    10141 tggttacatg gttggtccga atggcccggt taatctgcaa atcatcgtta gccaactgta
    10201 tgctgacgtg tcccagggca acgtgcgcta caacatcgcg accaaagcgg atatcgccat
    10261 catcgctacc gcgcagaatg gcaacaaaat gaccaaaaac tatcgtgcca gctacaacgt
    10321 tgaaggtgcg ttccaggcct ccaacaaaaa tatcgccgat gcggttaaca gtgtgctgac
    10381 cgataccatc gctgatatgt ctcaggacac cagcatccac gaattcatca agcagaacgc
    10441 gcgttaattt ctgccctctg gcccggtgca aaccgggcct gtagacgccc atgtccagtc
    10501 aatatttacg tatttttcaa cagccgcgtt cagccatatt gctgatcctg ggttttgctt
    10561 ccgggctacc gctcgccctg acatccggta ccttacaggc ctggatgacg gtcgagaata
    10621 ttgatctcaa aaccattggt ttcttctctc tggtaggcca ggcttacgtt tttaaattcc
    10681 tctggtcacc gctgatggac cgctacacgc ccccattttt tgggcggcgg cgcggttggc
    10741 tgctcgccac gcaaatcctg ttattagtcg ccattgcggc gatgggtttt ctcgaacctg
    10801 gcacccaact ccgctggatg gcggcactgg cggtggtgat cgctttttgc tcggcctccc
    10861 aggatattgt cttcgatgcg tggaaaaccg atgtgctccc ggcagaagaa cgtggtgcgg
    10921 gcgcggcaat cagcgtgctg ggttaccgtt tagggatgct ggtttccggc ggcttagccc
    10981 tgtggctggc agataaatgg ctgggctggc agggcatgta ctggttgatg gcggcgctgt
    11041 tgatcccttg tattatcgcg acgttgcttg caccagaacc gaccgacacc attcctgtgc
    11101 caaaaacgct ggaacaagcg gttgttgcac ctctgcgaga tttctttggt cgcaataatg
    11161 cctggcttat tttgcttctt atcgtgctgt ataagctggg cgacgcattc gccatgagcc
    11221 tgacaaccac gtttttgatt cgcggcgtcg ggtttgatgc gggtgaagta ggcgtggtta
    11281 acaaaacgct tggcttactg gcgaccattg tgggcgcatt gtacggtggg attttgatgc
    11341 agcgcctgtc gctgttccgg gcgctgctga ttttcggcat tttacaaggt gcgtctaacg
    11401 ctggttactg gctgctgtcg attactgata agcatctcta cagcatgggc gcagccgtct
    11461 ttttcgaaaa cctctgtggc gggatgggca catcagcctt tgtcgcgctg ttaatgacgc
    11521 tgtgtaataa gtcattttcc gctactcaat ttgccctgct ctcagcgctt tccgctgtag
    11581 gacgagttta tgtcggcccc gtggcgggtt ggtttgttga agcacacggc tggtcgacat
    11641 tctatctatt ctccgtcgcc gctgccgtac cggggcttat tttgctgctg gtttgccgcc
    11701 agacgcttga atatacacga gtaaatgaca actttatctc ccgtaccgaa tatccgacag
    11761 gttatgcctt tgccatgtgg acactggcgg cgggcgtcag cctgttggcc gtgtggttac
    11821 tgctgttgac gatggacgcg ctggatttga cgcacttctc tttcctgcct gctctgctgg
    11881 aagtcggggt tttagtcgcc ctttctggcg tcgtgcttgg tggtttgctg gattatctgg
    11941 ctctacgaaa aacgcatctg acgtaatctg taaatattat ttagctgtag taatcactcg
    12001 ccgtaattat tacggctaaa taaacatcag tatcgcttaa taatatttgt tcttttgtgc
    12061 ggcttagcgt ttggaaatta ttgacgcaat ttatgaattc tattttttct tacacgattc
    12121 agctaatgag tctttatttt cccccccacc caattgtcac cataatgata attatttgtt
    12181 aaacaattgt tttattccag attggttata ccaattgccc gtccaaactc cttctgcact
    12241 cccctttcgt tataacgccc ttttgcaaca gcttcttaaa atcaacttga tatgttttgc
    12301 aacatatgta acctgacagc caaatccaag taacaggaat ttaatcatgt ttacagtaat
    12361 gtaacctccc cgtaaaatgc ccacacactt taaacgccac cagatcccgt ggaattgagg
    12421 tcgttaaatg agactcagga aatacaataa aagtttggga tggttgtcat tatttgcagg
    12481 cactgtattg ctcagtggct gtaattctgc gctgttagat cccaaaggac agattggtct
    12541 ggagcaacgt tcactgatac tgacggcatt tggcctgatg ttgattgtcg ttattcccgc
    12601 aatcttgatg gctgttggtt tcgcctggaa gtatcgtgcg agcaataaag atgctaagta
    12661 cagcccgaac tggtcacact ccaataaagt ggaagctgtg gtctggacgg tacctatctt
    12721 aatcatcatc ttccttgcgg tactgacctg gaaaaccact cacgctcttg agcctagcaa
    12781 gccgctggca cacgacgaga agcccattac catcgaagtg gtttccatgg actggaaatg
    12841 gttcttcatc tacccggaac agggcattgc aaccgtgaat gaaatcgctt tcccggcgaa
    12901 cactccggtg tacttcaaag tgacctccaa ctccgtgatg aactccttct tcattccgcg
    12961 tctgggtagc cagatttatg ccatggccgg tatgcagact cgcctgcatc tgatcgccaa
    13021 cgaacccggt acttatgacg gtatctccgc cagctacagc gggccgggct tctcaggcat
    13081 gaagttcaaa gctattgcaa caccggatcg cgccgaattc gaccaatggg tcgcaaaagc
    13141 gaaacagtcg ccgaacacca tgtctgacat ggctgcgttc gaaaaactgg ccgcgcctag
    13201 cgaatacaac caggtggaat atttctccaa cgtgaaacca gacttgtttg ccgatgtgat
    13261 taacaagttt atggctcacg gtaagagcat ggacatgacc cagccagaag gtgagcacag
    13321 tgcacacgaa ggtatggaag gcatggacat gagccacgcg gaatccgccc attaaagggg
    13381 ttgaggaaga ataaagatgt tcggaaaatt atcacttgat gcagtcccgt tccatgaacc
    13441 tatcgtcatg gttacgatcg ctggcattat tttgggaggt ctggcgctcg ttggcctgat
    13501 cacttacttc ggtaagtgga cctacctgtg gaaagagtgg ctgacctccg tcgaccataa
    13561 acgcctcggt atcatgtata tcatcgtggc gattgtgatg ttgctgcgtg gttttgctga
    13621 cgccattatg atgcgtagcc agcaggctct tgcctcggcg ggcgaagcgg gcttcctgcc
    13681 acctcaccac tacgatcaga tcttcaccgc gcacggcgtg attatgatct tcttcgtggc
    13741 gatgcctttc gttatcggtc tgatgaacct ggtggttccg ctgcagatcg gcgcgcgtga
    13801 cgttgcgttc ccgttcctca acaacttaag cttctggttt actgttgttg gtgtgattct
    13861 ggttaacgtt tctctcggcg tgggcgaatt tgcgcagacc ggctggctgg cctatccacc
    13921 gctatcggga atagagtaca gtccgggagt cggtgtcgat tactggatat ggagtctcca
    13981 gctatccggt ataggtacga cgcttaccgg tatcaacttc ttcgttacca ttctgaagat
    14041 gcgcgcaccg ggcatgacca tgttcaagat gccagtattt acctgggcat cactgtgcgc
    14101 gaacgtactg attattgctt ccttcccaat tctgacggtt accgtcgcgt tgttgaccct
    14161 ggatcgctat ctgggcaccc atttctttac caacgatatg ggtggcaaca tgatgatgta
    14221 catcaacctg atttgggcct ggggccaccc ggaagtttac atcctgatcc tgcctgtttt
    14281 cggtgtgttc tccgaaattg cggcaacctt ctcgcgtaaa cgtctgtttg gttatacctc
    14341 gctggtatgg gcaaccgtct gtatcaccgt gctgtcgttc atcgtttggc tgcaccactt
    14401 ctttacgatg ggtgcgggcg cgaacgtaaa cgccttcttt ggtatcacca caatgattat
    14461 cgccatcccg accggggtga agatcttcaa ctggctgttc accatgtatc agggccgcat
    14521 cgtgttccat tctgcgatgc tgtggaccat cggttttatc gtcactttct cggtgggcgg
    14581 gatgactggc gtgctgctgg cagtaccggg cgcggacttc gttctgcata acagcctgtt
    14641 cctgattgcg cacttccata acgttatcat cggcggcgtg gtcttcggct gcttcgcagg
    14701 gatgacctac tggtggccta aagcgttcgg tttcaaactg aatgaaacct ggggtaaacg
    14761 cgcgttctgg ttctggatca tcggcttctt cgttgccttt atgccgctgt atgcgctggg
    14821 cttcatgggc atgacccgtc gtttgagcca gcagattgac ccgcagttcc acaccatgct
    14881 gatgattgca gctagcggtg cagtactgat tgcgctgggt attctctgcc tcgttattca
    14941 gatgtacgtt tctattcgcg accgcgacca gaaccgtgac ctgactggcg acccgtgggg
    15001 tggccgtacg ctggagtggg caacctcttc cccgcctccg ttctataact ttgccgttgt
    15061 gccgcacgtt cacgagcgtg atgcattctg ggaaatgaaa gagaaaggcg aagcgtataa
    15121 aaagcctgac cactatgaag aaattcatat gccgaaaaac agcggtgcag gtatcgtcat
    15181 tgcagctttc tccaccatct tcggtttcgc catgatctgg catatctggt ggctggcgat
    15241 tgttggcttc gcaggcatga tcatcacctg gatcgtgaaa agcttcgacg aggacgtgga
    15301 ttactacgtg ccggtggcag aaatcgaaaa actggaaaac cagcatttcg atgagattac
    15361 taaggcaggg ctgaaaaatg gcaactgata ctttgacgca cgcgactgcc cacgcgcacg
    15421 aacacgggca ccacgatgca ggcggaacca aaatcttcgg attttggatc tacctgatga
    15481 gcgactgcat tctgttctca atcttgtttg ctacctatgc cgttctggtg aacggcaccg
    15541 caggcggccc gacaggtaag gacattttcg aactgccgtt cgttctggtt gaaactttct
    15601 tgctgttgtt cagctccatc acctacggca tggcggctat cgccatgtac aaaaacaaca
    15661 aaagccaggt tatctcctgg ctggcgttga cctggttgtt tggtgccgga tttatcggga
    15721 tggaaatcta cgaattccat cacctgattg ttaacggcat gggtccggat cgcagcggct
    15781 tcctgtcagc gttctttgcg ctggtcggca cgcacggtct gcacgtcact tccggtctta
    15841 tctggatggc ggtgctgatg gtacaaatcg ctcgtcgcgg cctgaccagc actaaccgta
    15901 cccgcatcat gtgcctgagc ctgttctggc acttcctgga tgtggtttgg atctgtgtgt
    15961 tcactgttgt ttatctgatg ggggcgatgt aatgagtcat tctaccgatc acagcggcgc
    16021 gtcccatggc agcgtaaaaa cctacatgac aggctttatc ctgtcgatca ttctgacggt
    16081 gattccgttc tggatggtga tgacaggagc tgcctctccg gccgtaattc tgggaacaat
    16141 cctggcaatg gcagtggtac aggttctggt gcatctggtg tgcttcctgc acatgaatac
    16201 caaatcagat gaaggctgga acatgacggc gtttgtcttc accgtgctaa tcatcgctat
    16261 cctggttgta ggctccatct ggattatgtg gaacctcaac tacaacatga tgatgcacta
    16321 agagcggcgg ttatgatgtt taagcaatac ctgcaagtaa cgaaaccagg catcatcttt
    16381 ggcaacctga tctcggtgat tgggggattc ctgctggcct caaagggcag cattgattat
    16441 cccctgttta tctacacgct ggttggggtg tcactggttg tggcgtcggg ttgtgtgttt
    16501 aacaactaca tcgacaggga tatcgacaga aagatggaaa ggacgaagaa tcgggtgctg
    16561 gttaaaggcc tgatctctcc tgctgtctcg ctggtgtacg ccaccttgct aggctttgct
    16621 ggctttatgc tgctgtggtt tggcgcgaat ccgctggcct gctggctggg ggtgatgggc
    16681 tttgtggttt atgtcggcgt ttatagcctg tacatgaaac gccactcggt ctacggcaca
    16741 ttgattggtt cgctctccgg cgctgcgccg ccggtgattg gctactgtgc agtaaccggc
    16801 gagttcgata gcggcgcagc gatcctgctg gctatcttca gcctgtggca gatgcctcac
    16861 tcctatgcca tcgccatttt ccgctttaag gattatcagg cggcaaacat tccggtattg
    16921 ccagtggtaa aaggcatttc ggtggcgaag aatcacatca cgctgtatat catcgccttt
    16981 gccgttgcca cgctgatgct ctctcttggc ggttacgctg ggtataaata tctggtggtc
    17041 gccgcggcgg tgagcgtctg gtggttaggt atggccctgc gcggttataa agttgctgat
    17101 gacaggatct gggcgcgcaa gctgttcggc ttctctatca tcgccatcac agccctctcg
    17161 gtgatgatgt ccgttgattt tatggtgccg gactcgcata cgctgctggc tgctgtttgg
    17221 taacacaacc tctctattaa aaaggtgcta cggcaccttt tttattgtct gagatcagag
    17281 atacggctaa taaccgtatg tcttgacaga gtgagcagat gaacataaat taatcatacg
    17341 gttgatagcc gtattatgta tgagatcgac tccctacgat ttcattcagt taatcaaaga
    17401 ggtcataatg cctggaaaaa cagccactct acccgatgtc gataaaacgc tgaaaaatgc
    17461 gcgggtcgaa ctcaaaacta gtcccgacgc caaaaacaaa cttcgtgaag ctgcgcaagc
    17521 cgttggggta gatttaagcg cttttatttt gagcgctgct atggaacgcg ccgaaagtgt
    17581 gcttgataac caacgtcgcc gtgagctttc gaatcaaagc tgggaactga tgaaccaact
    17641 catcgctgaa cctgctcaac cgacgctcgc cctcaaggcg ttaatgaaaa ggaaaaacag
    17701 cgatggtcga caagcatgaa gagattactc tgcccatagt cctctcctgt aattatcagt
    17761 ctgatattac ttatcctggg caaaaacagt ttgattgcgg taactctgtt atcgataaat
    17821 ttgtacgcgc atcgctaaag aaaagtgtgc gtaatagcga ctgtgcggct aaagcactta
    17881 ttgacagaca aagtggtgaa ctgatcggca tctgtacttt tacggcatat tcgctggaaa
    17941 aacaacgcgt ttctggcgtc cttcagggtt cacaaccttc agaaattggt gttgtcagat
    18001 tagtcatgtt gggggtagca cggaagtatc aaaagcgggg ctttggtcag gacctactat
    18061 gtgatttttt tgaacatgta aaaataattc accaggcatt accaattaaa ggggtttatc
    18121 ttgatgctga ccctgccgcc attaattttt atgctcgtct cggctttgtt cagctttcag
    18181 cgacaccaaa tgcttttggt gctgtaccta tgtttttggc gattcagcat attctcgcgg
    18241 cttagattat tatcgccacc cgccagggca agttttgcct tagccggggg ctgagcagcg
    18301 ttaccacttt tgttcaattg cccccgcttt cagcaactcc acatccttgc cgttaatggt
    18361 tgccgttgcg gcaacgattg tggtccttac agttgcacaa gattttcgct caaattccgg
    18421 tttataaaga ataagagacg aattactggt aaatgaacca aataggtaat cagtaaagct
    18481 aatattcagc cggtttaaac cgaaaggcgc gaagccaacc gtttggttaa tctccttacc
    18541 ggatgggcga cgaatgctga atgtcacgcg aagattggcc acttcgccta atttattggt
    18601 ggtggcgaag aggctatcca tgcaggaaaa acctttatct aaaaaattat ctcgcatccc
    18661 agaacgggta accgtaatca cgggtaacgt atcgtcaacc gtttttctgt caatcatgtc
    18721 ttttcgacaa gtagccagaa agtcttccgg gccaccataa atgtggacac ctttatcaag
    18781 atattccttc gctttcttac aatctacctc aacatactga ccattggcgt acataatgcc
    18841 c
//
LOCUS       DAESHI010000081        18786 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.082, whole genome shotgun sequence.
ACCESSION   DAESHI010000081 DAESHI010000000
VERSION     DAESHI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18786
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.082"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(166..576)
                     /locus_tag="KW011_002894"
     CDS             complement(166..576)
                     /locus_tag="KW011_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HBH7661181.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTVLIKGQGKVVNVRFSKNKCLIEATLKGFKSGELSFYEYRKNLATAGVFR
                     WITNIHENKRYYYTFDNSLLFTENIQNTTQIFPH"
     gene            complement(634..867)
                     /locus_tag="KW011_002895"
     CDS             complement(634..867)
                     /locus_tag="KW011_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HBH7661182.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            1256..1801
                     /locus_tag="KW011_002896"
     CDS             1256..1801
                     /locus_tag="KW011_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HBH7661183.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1776..3701
                     /locus_tag="KW011_002897"
     CDS             1776..3701
                     /locus_tag="KW011_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HBH7661184.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3698..3904
                     /locus_tag="KW011_002898"
     CDS             3698..3904
                     /locus_tag="KW011_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HBH7661185.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3901..5502
                     /locus_tag="KW011_002899"
     CDS             3901..5502
                     /locus_tag="KW011_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBH7661186.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            5483..6802
                     /locus_tag="KW011_002900"
     CDS             5483..6802
                     /locus_tag="KW011_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HBH7661187.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6812..7144
                     /locus_tag="KW011_002901"
     CDS             6812..7144
                     /locus_tag="KW011_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HBH7661188.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTVF
                     AGTAISIV"
     gene            7200..8225
                     /locus_tag="KW011_002902"
     CDS             7200..8225
                     /locus_tag="KW011_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBH7661189.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            8267..8665
                     /locus_tag="KW011_002903"
     CDS             8267..8665
                     /locus_tag="KW011_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBH7661190.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            8677..9030
                     /locus_tag="KW011_002904"
     CDS             8677..9030
                     /locus_tag="KW011_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HBH7661191.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            9042..9620
                     /locus_tag="KW011_002905"
     CDS             9042..9620
                     /locus_tag="KW011_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH7661192.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9617..10012
                     /locus_tag="KW011_002906"
     CDS             9617..10012
                     /locus_tag="KW011_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH7661193.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            10020..10760
                     /locus_tag="KW011_002907"
     CDS             10020..10760
                     /locus_tag="KW011_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH7661194.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            10776..11198
                     /locus_tag="KW011_002908"
     CDS             10776..11198
                     /locus_tag="KW011_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBH7661195.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAIFHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            11180..11614
                     /locus_tag="KW011_002909"
     CDS             11180..11614
                     /locus_tag="KW011_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBH7661196.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            11607..14168
                     /locus_tag="KW011_002910"
     CDS             11607..14168
                     /locus_tag="KW011_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBH7661197.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            14165..14494
                     /locus_tag="KW011_002911"
     CDS             14165..14494
                     /locus_tag="KW011_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH7661198.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            14494..15192
                     /locus_tag="KW011_002912"
     CDS             14494..15192
                     /locus_tag="KW011_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBH7661199.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            15198..15941
                     /locus_tag="KW011_002913"
     CDS             15198..15941
                     /locus_tag="KW011_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH7661200.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            15878..16510
                     /locus_tag="KW011_002914"
     CDS             15878..16510
                     /locus_tag="KW011_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBH7661201.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRAMATQLPAFRQKLSD
                     GWYQVRIAGQDVSTSGLTAQLHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     IYFSSLDNMVAQGNVMPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16571..>18786
                     /locus_tag="KW011_002915"
     CDS             16571..>18786
                     /locus_tag="KW011_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBH7661202.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWAYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     HQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAALSRIELTPGYFQITATPHLAVY"
BASE COUNT         4275 a         4703 c         5841 g         3967 t
ORIGIN      
        1 gtcgccgtat tcccgtatta acagagaccg tagcccgacg gggaactcct tctgcgcgag
       61 tgtgcgggaa taatcaaaaa cgatgcacac cggggttacc gggtacacat atttcatcat
      121 gccagcgagt ccggttctgg cacggaagaa accggacgtt atgatttagt gcggaaatat
      181 ttgtgtagtg ttctgtatgt tctcagtaaa gagtaatgaa ttatcaaagg tatagtaata
      241 ccttttgttt tcgtggatat ttgtaatcca tctgaaaacc cctgctgtag caagattttt
      301 cctgtattcg taaaatgata actctcctga tttgaatcct tttaaggtgg cttctataag
      361 gcatttattt tttgaaaatc ttacatttac aaccttaccc tgtcctttta ttaaaaccgt
      421 attatcgttt tcaagaacaa gatgaatatt ctctgtggct aaatagtaaa tgtaatgtga
      481 gacattgtga cgttttagtt cagaataaaa ccagtgatag tttaaattat ttcgcacttt
      541 atcgaatatt tgtttaaaaa tggcaacctg agccattgta gtaccttcca tgtgatatga
      601 gggggcgtag tctgcacgat tatctaaatt gcttcaatct ggtctaacct gttttctgag
      661 caattcagta atgtcactct tttctttgtt tgcttcaggc gaaactcttt tttctgagca
      721 cagtctccgg cggcaggctt caatgaccca ggctgagaaa ttcccggacc ctttttgaac
      781 aagagcgatg ttaatttgtt caatcatttg gttaggaaag cggatgttgc gggttgttgt
      841 tctgcgggtt ctgttcttcg ttgacatgag gttgccctgt attcagtgtc gctgatttgt
      901 attgtctgaa gttgttttta cgttaagttg atgcagatca attaatacga tacctgcgtc
      961 ataattgatt atttgacgtg gtttgatggc gtagatgcac gttgtgacat gtagatgata
     1021 attattatca ttttgcgggt cctttccggc gatccgacag gttacggggc ggaaggcgcg
     1081 cgggttttcg ctatttatga caattttccg gtttaaggcg tttccgttct tcttcgccgt
     1141 aacctaatgt ttttatttaa aacaccccct gaaaagaaag gaaacgacag gtgctgaaaa
     1201 cgggcttttt ggcctctgtc gtttcctttc tctgtttttg tccgtggaat gaacaatgga
     1261 agtcaacaaa aagcagctgg ctgacatttt cggtgcgagt atccgtacca ttcagaactg
     1321 gcaggaacag ggaatgcccg ttctgcgagg cggtggcaag ggtaatgagg tgctttatga
     1381 ctctgccgcc gttataagat ggtatgccga aagggatgct gaaattgaga acgaaaagct
     1441 gcgccgggaa gttgaagaac tgcggcaggc cagcgagaca gatctccagc cagggactat
     1501 tgagtacgaa cgccatcgac ttacgcgtgc gcaggccgac gcacaggagc tgaaaaatgc
     1561 cagagactcc gctgaagtgg tggaaaccgc attctgtact ttcgtgctgt cgcggatcgc
     1621 aggtgaaatt gccagtattc tcgacgggat ccccctgtcg gtgcagcggc gttttccgga
     1681 actggaaaac cgacatgttg atttcctgaa acgggatatc atcaaagcca tgaacaaagc
     1741 agccgcgctg gatgaactga taccggggtt gctgagtgaa tatatcgaac agtcaggtta
     1801 acaggctgcg gcattttgtc cgcgccgggc ttcgctcact gttcaggccg gagccacaga
     1861 ccgccgttga atgggcggat gctaattact atctcccgaa agaatccgca taccaggaag
     1921 ggcgctggga aacactgccc tttcagcggg ccatcatgaa tgcgatgggc agcgactaca
     1981 tccgtgaggt gaatgtggtg aagtctgccc gtgtcggtta ttccaaaatg ctgctgggtg
     2041 tttatgccta ctttatagag cataagcagc gcaacacact tatctggttg ccgacggatg
     2101 gtgatgccga gaactttatg aaaacccacg ttgagccgac catccgcgat attccgtcgc
     2161 tgctggcgct ggctccgtgg tatggcaaaa agcaccggga taacacgctc actatgaagc
     2221 gtttttccaa tggtcgtggc ttctggtgcc tgggcggtaa agcggcaaaa aactaccgtg
     2281 aaaagtcggt ggatgtggcg ggttatgatg aacttgctgc ctttgatgag gatattgaac
     2341 aggaaggctc tccgacgttc cttggcgaca aacgtattga aggctcggtc tggccaaagt
     2401 ccatccgtgg ctccacgccc aaagtgagag gcacctgcca gattgagcgt gcagccagtg
     2461 aatccccgca ttttatgcgt tttcatgttg cctgcccgca ctgcggggag gagcagtatc
     2521 ttaaatttgg cgacaaagag acgccgtttg gcctcaaatg gacgccggat gacccctcca
     2581 gcgtgtttta tctctgcgag cataatgcct gcgtcatccg ccagcaggag ctggacttta
     2641 ctgatgcccg ttatatctgc gaaaagaccg ggatctggac ccgtgatggc attctctggt
     2701 tttcgtcatc cggtgaagag attgagccac ctgacagtgt gacctttcac atctggacag
     2761 cgtacagccc gttcaccacc tgggtgcaga ttgtcaaaga ctggatgaaa acgaaagggg
     2821 atacgggaaa acgtaaaacc ttcgtaaaca ccacgctcgg tgagacgtgg gaggcgaaaa
     2881 ttggcgaacg tccggatgct gaagtgatgg cagagcggaa agagcattat tcagcgcccg
     2941 ttcctgaccg tgtggcttac ctgaccgccg gtatcgactc ccagctggat cgctacgaaa
     3001 tgcgcgtatg gggatggggg ccgggtgagg aaagctggct gattgatcgg cagattatta
     3061 tgggccgcca cgacgatgaa cagacgctgc tgcgtgtgga tgaggccatc aataaaacct
     3121 atacccgccg gaatggtgca gaaatgtcga tatcccgtat ctgctgggat attggcggga
     3181 ttgacccgac cattgtgtat gaacgctcga aaaagcatgg gctgttccgg gtgatcccca
     3241 ttaaaggggc atccgtctac ggtaagccgg tggccagcat gcctcgtaag cgaaacaaaa
     3301 acggggttta ccttaccgaa atcggtacgg ataccgcgaa agagcagatt tataaccgct
     3361 tcacactgac gccggaaggg gatgaaccgc ttcccggtgc cgttcacttc ccgaataacc
     3421 cggatatttt tgatctgacc gaagcgcagc agctgactgc tgaagagcag gtcgaaaaat
     3481 gggtggatgg caggaaaaaa atactgtggg acagcaaaaa gcgacgcaat gaggcgctcg
     3541 actgcttcgt ttatgcgctg gcggcgctgc gcatcagtat ttcccgctgg cagctggatc
     3601 tcagtgcact gctggcgagc ctgcaggaag aggatggtgc agcaaccaac aagaaaacac
     3661 tggcagatta cgcccgtgcc ttatccggag aggatgaatg acgcgacagg aagaacttgc
     3721 cgctgcccgt gcggcactgc atgacctgat gacaggtaaa cgggtggcaa cggtacagaa
     3781 agacggacgg cgagtggagt ttacggccac ttccgtgtct gacctgaaaa aatacattgc
     3841 ggagctggag gtgcagaccg gcatgacaca gcgacgcagg ggacctgcag gattttatgt
     3901 atgaaaacgc ccaccattcc cacccttctg ggaccggacg gcatgacatc gctgcgtgaa
     3961 tatgccggtt atcacggcgg tggcagcgga tttggtgggc agttgcgggc gtggaaccca
     4021 ccgggtgaaa gtgtggatgc agccctgctg cccaacttta cccgtggcaa tgcccgcgca
     4081 gacgatctgg tacgcaataa cggctatgct gccaacgcca tccagctgca tcaggatcat
     4141 atcgtcgggt cttttttccg gctcagtcat cgcccaagct ggcgctatct gggcatcggg
     4201 gaggaagacg cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga gtttgccgag
     4261 gatgactgct gctgcattga cgttgagcga aaacgcacgt ttaccatgat gattcgggaa
     4321 ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc aggccacctg ggataccagt
     4381 tcgtcgcggc ttttccggac acagttccgg atggtcagcc cgaagcgcat cagcaacccg
     4441 aacaataccg gcgacagccg gaactgccgt gccggtgtgc agattaatga cagcggtgcg
     4501 gcgctgggat attacgtcag cgaggacggc tatcctggct ggatgccgca gaaatggaca
     4561 tggatacccc gtgagttacc cggcgggcgc gcctcgttca ttcacgtttt tgaacccgtg
     4621 gaggacgggc agacccgcgg tgcaaatgtg ttttacagcg taatggagca gatgaagatg
     4681 ctcgacacgc tgcagaacac gcagctgcag agcgccattg tgaaggcgat gtatgccgcc
     4741 accattgaaa gtgagctgga tacgcagtca gcgatggatt ttattctggg cgcgaacagt
     4801 aaggagcagc gggaaaggct gacgggctgg attggtgaaa ttgccgcgta ttacgccgca
     4861 gcaccggtcc gtctgggagg cgcaaaagtg ccgcacctga tgccggggga ctcactgaac
     4921 ctgcagacgg ctcaggacac ggataacggc tactccgtgt ttgagcagtc actgttgcgg
     4981 tatatcgctg ccgggctggg tgtctcgtat gagcaacttt cccggaatta cgcccagatg
     5041 agctactcca cggcacgggc cagtgcgaac gagtcgtggg cgtactttat ggggcggcga
     5101 aaattcgtcg catcccgtca ggcgagccag atgtttctgt gctggctgga agaggccatc
     5161 gttcgccgcg tggtgacgtt accttcaaaa gcgcgcttca gctttcagga agcccgcagt
     5221 gcctggggga actgcgactg gataggctcc ggtcgtatgg ccatcgatgg tctgaaagaa
     5281 gttcaggaag cggtgatgct gatagaagcc ggactgagca cctacgagaa agagtgcgcg
     5341 aaacgcggtg acgactatca ggaaattttt gcccagcagg tccgtgaaac gatggagcgc
     5401 cgcgcagctg gtcttaaacc gcccgcctgg gcggctgcgg catttgaatc cgggctgcga
     5461 caatcaacag aggaggagaa gagtgacagc agagctgcgt aatctcccgc atattgccag
     5521 catggctttt aatgagccgc tgatgcttga acccgcctat gcgcgggttt tcttttgtgc
     5581 gcttgcaggc cagcttggga tcagctgcct gacggatgca gtatccggcg acagcctgac
     5641 tgccggagag gcacccgcgg cgctggcgtt atccggtgat gatgacggac cacgacaggc
     5701 ccgcagttat caggtaatga acggcatcgc cgtgctgccg gtgtccggca cgctggtcag
     5761 ccggacgcgg gcgctgcagc cgtactcggg gatgaccggt tacaacggca ttatcgcccg
     5821 tctgcaacag gctgccagcg acccgatggt ggacggcatt ctgctcgata tggacacacc
     5881 gggcggaatg gtggcgggag catttgactg cgctgacatc atcgcccgtg tgcgtgacat
     5941 aaagccggta tgggcgctgg ccaatgacat gaactgcagc gcaggtcagc tgcttgccag
     6001 tgccgcctcc cggcgtctgg tcacgcagac cgcccggaca ggctccatcg gcgtcatgat
     6061 ggctcacagt aattacggcg ctgcactgga gaaacagggc gtggaaatca cgctgattta
     6121 cagcggcagc cataaggtgg atggcaaccc ctacagccat ctaccggatg atgtccggga
     6181 aacattgcag tcccggatgg atgcaacccg ccggatgttt gcacagaagg tgtcggcata
     6241 taccggcctg tccgtgcagg ctgtgctgga taccgaggct gcagtgtaca gcggtcagga
     6301 ggccattgat gccggactgg ctgatgaact tgttaacagt accgatgcga tcaccgtcat
     6361 gcgtgatgca ctggatgcac gtaaatcccg tctctcagga gggcgaatga ccaaagagac
     6421 tcaatcaaca actgtttcag ccactgcttc gcaggctgac gttactggcg tggtgcaagc
     6481 gacggagggc gagaacgcca gcgcggcgca gccggacgtg aacgcgcaga tcaccgcagc
     6541 ggttgcggca gaaaacagtc gcattatggg gatcctcaac tgtgaggagg ctcacggacg
     6601 cgaagaacag gcacgcgtgc tggccgaaac ccccggtatg accgtggaaa cggcccgccg
     6661 tattctggcc gcagcaccac agagtgcaca gacgcgcagt gacactgcgc tggatcgtct
     6721 gatgcagggg gcaccggcac cgctggctgc aggtaacccg gcatctgatg ccgttaacga
     6781 tttgctgaac acaccagtgt aagggatgtt tatgacgagc aaagaaacct ttacccatta
     6841 ccagccgctg ggcaatagtg acccggctca taccgcaacc gcgcccggcg gattgagtgc
     6901 gaaagcgcct gcaatgaccc cgctgatgct ggacacctcc acccgtaagc tggttgcgtg
     6961 ggatggcacc accgacggtg ctgccgttgg cattcttgcg gttgctgctg accagaccag
     7021 caccacactg acgttctaca agtccggcac gttccgttat gaggatgtgc tctggccgga
     7081 ggctgccagc gacgagacga aaaaacggac cgtgtttgcc ggaacggcaa tcagcatcgt
     7141 ttaactttac ccttcatcac taaaggccgc ctgtgcggct ttttttacgg gattttttta
     7201 tgtcgatgta cacaaccgcc cagctgctgg cggcaaatga gcagaaattt aagtttgatc
     7261 cgctgtttct gcgtctcttt ttccgtgaga gctatccctt cactacggag aaagtctatc
     7321 tctcacaaat tccgggactg gtaaacatgg cgctgtacgt ttcgccgatt gtttccggtg
     7381 aggttatccg ttcccgtggc ggctccacct ctgaatttac gccgggatat gtcaaaccca
     7441 agcatgaggt gaatccgcag atgaccctgc gtcgcctgcc ggatgaagat ccgcagaatc
     7501 tggcggaccc ggcttaccgc cgccgtcgca tcatcatgca gaacatgcgt gacgaagagc
     7561 tggccattgc tcaggtcgaa gagatgcagg cagtttctgc cgtgctcaag ggcaaataca
     7621 ccatgaccgg tgaagccttc gatccggttg aggtggatat gggccgcagt gcggcgaaca
     7681 acatcacaca gtccggcgtc acggagtgga gcaagcgtga caagtccacg tatgacccga
     7741 ccgacgatat cgaagcctac gcgctgaacg ccagcggtgt ggtgaatatc atcgtgtttg
     7801 acccgaaagg ctgggcgctg ttccgttcct tcaaagccgt caaggagaag ctggataccc
     7861 gtcgcggctc tcattccgag ctggagacag cggtaaaaga cctgggcaaa gcggtgtcct
     7921 ataagggaat gtatggcgat gtggccatcg tcgtgtattc cggacagtac gtggaaaacg
     7981 gcgtcaaaaa gaacttcctg ccggacaaca cgatggtgct ggggaacact caggctcgcg
     8041 gtctgcgcac ctatggctgc attcaggatg cggacgcaca gcgcgaaggc attaacgcct
     8101 ctgcccgtta cccgaaaaac tgggtgacca ccggcgatcc ggcgcgtgag ttcaccatga
     8161 ttcagtcagc accgctgatg ctgctggctg atcctgatga gttcgtgtcc gttcaactgg
     8221 cgtaatcatg gcccttcggg gccattttct ctctgtggag gagttcatga cgaaagatga
     8281 actgattgcc cgtcttcagg agctgggtga gcaattgaac cgcgatgtca gtttgacggg
     8341 aacgaaagaa gaactggcgc ttcgtgtggc agagctggaa gaagagcttg atgacacgga
     8401 tgaaactgcc ggtcaggaca cccctctcag ccgggaaaat gtgctgaccg ggcatgaaaa
     8461 tgaggtggta tcagcgcagc cggataccgt gattcaggat acggctgaac tggtcacggt
     8521 cgtggcactg gtgacgctgc atactgatgc acttcacgcc acgcgggata aacctgtggc
     8581 atttgtgctg ccgggaacgg cgtttcgtgt ctctgccggt gtggcagccg aaatgacaga
     8641 gcgcggcctg gccagaatgc aataacggga ggcgctgtgg ctgatttcga taacctgttc
     8701 gatgctgcca ttgcccgcgc cgatgaaacg atacgcgggt acatgggaac gtcagccacc
     8761 atgacatccg gtgagcagtc aggtgcggtg atacgtggtg tttttgatga ccctgaaaat
     8821 atcagctatg ccggacaggg cgtgcgcgtt gaaggctcca gcccgtccct gtttgtccgg
     8881 actgatgagg tgcggcagct gcggcgtgga gacacgctga ccatcggtga ggaaaatttc
     8941 tgggtagatc gggtttcgcc ggatgatggc ggaagttgtc atctctggct tggacggggc
     9001 gtaccgcctg ccgttaaccg tcgccgctga aagggggatg tatggccata aaaggtcttg
     9061 agcaggccgt tgaaaacctc agccgtatca gcaaaacggc ggtgcccggt gccgccgcaa
     9121 tggccattaa ccgcgttgct tcatccgcga tatcgcagtc tgcgtcacag gttgcccgtg
     9181 agacaaaggt acgccggaaa ctggtaaagg aaagggccag gctgaaaagg gccacggtca
     9241 aaaatccgca ggccagaatc aaagttaacc ggggggattt gcccgtaatc aagctgggta
     9301 atgcgcgggt tgtcctgtcc cgccgcaggc gtcataaaaa ggggcagcgt tcatccctga
     9361 aaggtggcgg cagcgtgctt gtggtgggta accgtcgtat tcccggcgcg tttattcagc
     9421 aactgaaaaa cggccggtgg catgttatgc agcgtgtggc cgggaaaaac cgttacccca
     9481 ttgatgtggt gaaaatcccg atggcggtgc cgctgaccac ggcgtttaaa cagaatattg
     9541 aacggatacg gcgtgaacgt cttccgaaag agctgggcta tgcgctgcag catcaactga
     9601 gaatggtaat aaagcgatga aacatactga actccgtgca gccgtactgg atgcactgga
     9661 gaagcatgac accggggcga cgctttttga tggtcgcccc gctgtttttg atgaggaaga
     9721 ttttccggca attgccgttt atctcaccgg cgctgaatac acgggcgaag agctggacag
     9781 cgatacctgg caggcggagc tgcatattga agttttcctg cctgctcagg tgccggattc
     9841 agagctggat tcgtggatgg agtcccggat ttatccggtg atgagcgata tcccggcact
     9901 gtcagatttg atcaccagta tggtggccag cggctatgac taccggcgcg acgatgatgc
     9961 gggcctgtgg agttcagccg atctgactta tgtcattatc tatgaaatgt gaggacgcta
    10021 tgcctgtacc aaatcctaca atgccggtga aaggtgccgg gaccaccctg tgggtttata
    10081 aggggagcgg tgacccttat gcgaacccgc tttcagacgt tgactggtcg cgtctggcaa
    10141 aagttaaaga cctgacgccc ggcgaactga ccgctgagtc ctatgacgac agctatctcg
    10201 atgatgaaga tgcagactgg actgcgaccg ggcaggggca gaaatctgcc ggagatacca
    10261 gcttcacgct ggcgtggatg cccggagagc aggggcagca ggcgctgctg gcgtggttta
    10321 atgaaggtga tacccgtgcc tataaaatcc gcttcccgaa cggcacggtc gatgtgttcc
    10381 gtggctgggt cagcagtatc ggtaaggcgg tgacggcgaa ggaggtgatt acccgcacgg
    10441 tgaaggtcac caatgtggga cgtccgtcga tggcagaaga tcgcagcacg gtgacggcga
    10501 cgaccggcat gacggtgaca cccgccagtg cttccgtagt gaaagggcag agcaccacgc
    10561 tgaccgtggc attccagccg gatggcgcaa ccgacaagag cttccgtgcg gtgtctgcgg
    10621 ataaaacaaa agccaccgtg tcggtcagtg gtatgaccat caccgtgaaa ggtgttgctg
    10681 caggcaaggt caacattccg gtcgtatctg gtaatggtga gtttgctgcg gttgcagaaa
    10741 tcaacgtcac cgccagttaa tccggagagt cagcgatgtt cctgaaaacc gaatcatttg
    10801 aacataacgg tgtgaccgtc acgctttctg aactgtcagc cctgcagcgt attgagcatc
    10861 tcgacctgat gaaacggcag gcagaacagg cggagtcaga cagcaaccgg aagtttactg
    10921 tggaagacgc catcagaacc ggcgcttttg tggttgcgat gtccctgtgg cataaccatc
    10981 cgaagaagac gcagatgccg tccatgaatg aagccgttaa acagattgag caggaagtgc
    11041 ttaccacctg gcccacagag gcaatttttc atgctgaaaa cgtggtgtac cggctgtccg
    11101 gtatgtatga gtttgtggtg aatgatgctc ctgaacaggc agaggacgcc gggcctgcag
    11161 agcctgtttc tgcgggaaag tgttcgacgg tgagctgagt tttgccctga aactggcgcg
    11221 tgagatgggg cgacccgact ggcgcgccat gcttgccggg atgtcatcca cggagtatgc
    11281 cgactggcac cgcttttaca gtacccatta ttttcatgat gttctgctgg atatgcactt
    11341 ttccgggctg acgtacaccg tgctcagcct gtttttcagc gatccggaga tgcatccgct
    11401 ggatttcagt ctgctgaacc ggcgcgaggc tgacgaagag cctgaagatg atgtgctgat
    11461 gcagaaagcg gcagggcttg ccggaggtgt ccgctttggc ccggacggga atgaagttat
    11521 ccccgcttcc ccggatgtgg cggacatgac ggaggatgac gtaatgctga tgacagtatc
    11581 agaagggatc gcaggaggag tccggtatgg ctgaaccggt aggcgatctg gtcgttgatt
    11641 tgagtctgga tgcggccaga tttgacgagc agatggccag agtcaggcgt catttttccg
    11701 gtacggaaag tgatgcgaaa aaaacagcgg cagtcgttga acagtcgctg agccgacagg
    11761 cgctggctgc acagaaagcg gggatttccg tcgggcagta taaagccgcc atgcgtatgc
    11821 tgcctgcaca gttcaccgac gtggccacgc agcttgcagg ggggcagaat ccctggctga
    11881 tcctgctgca acagggtggt caggttaagg actccttcgg cgggatgatc cccatgttca
    11941 gggggcttgc cggcgcgatc accctgccga tggtgggggc cacctcgctg gcggtggcga
    12001 ccggtgcgct ggcgtatgcc tggtatcagg gcaactcaac cctgtccgat ttcaacaaaa
    12061 cgctggtcct ttccggtaat caggcgggac tgacggcaga tcgtatgctg gtcctgtcca
    12121 gagccgggca ggcggcaggg ctgacgttta accagaccag cgagtcactc agcgcactgg
    12181 ttaaggcggg ggtaagcggt gaggctcaga ttgcgtccat cagccagagt gtggcgcgtt
    12241 tctcctctgc atccggcgtg gaggtggaca aggtcgctga agccttcggg aagctgacca
    12301 cagacccgac gtcggggctg acggcgatgg cacgccagtt ccataacgtg acggcggagc
    12361 agattgcgta tgttgctcag ttgcagcgtt ccggcgatga agccggggca ttgcaggcgg
    12421 cgaacgaggc cgcaacgaaa gggtttgatg accagacccg ccgcctgaaa gagaacatgg
    12481 gcacgctgga gacctgggca gacaggactg cacgtgcatt caaatccatg tgggatgcgg
    12541 tgctggatat tggtcgtcct gataccgcgc aggagatgct gattaaggca gaggctgcgt
    12601 ttaagaaagc agacgacatc tggaatctgc gcaaggatga ttatttcgtt aacgatgaag
    12661 cacgggcgcg ttactgggat gatcgtgaaa aaaaacgcct ggagcgggat gcagcccaaa
    12721 agagggtaga tcaacagcgt caacaggaca aaaatgcgca gcagcagagc gataccgaag
    12781 cgtcacggct gaaatatacc gaagaggcgc agaaggctta cgaacggctg cagacgccgc
    12841 tggagaaata taccgcccgt caggaagaac tgaacaaggc actgaaagac ggaaaaatcc
    12901 tgcaggcaga ttacaacacg ctgatggcgg cggcgaaaaa ggattatgaa gcgacgctga
    12961 aaaagccgaa acagtccggc gtgaaggtgt atgcgggcga tcgtcaggaa gacagtgctc
    13021 atgcggccct gctgacgctt caggcagaac tccggacgct ggagaagcat gccggagcga
    13081 atgagaaaat cagccagcag cgccgggatt tgtggaaggc agaaagtcag ttcgcggtac
    13141 tggaggaggc ggcacaacgt cgccagctgt ccgcacagga gaaatccctg ctggcgcata
    13201 aagacgagac gctggagtac aaacgccagc tggctgcact tggtgacaag gttacgtatc
    13261 aggagcacct gaatgcgctg gcgcagcagg cggataaatt cgcacagcag caacgggcaa
    13321 aacgggcagc cattgatgcg aaaaaccggg ggctgactga ccggcaggca gcgcgggaag
    13381 ccacggaaca gcgcctgaag gaacagtatg gcgataatcc tctggcgctg aataacgtca
    13441 tgtcagagca gaaaaagacc tgggcggctg aagaccagct tcgcgggagc tggatggcag
    13501 gcctgaagtc cggctggagt gagtgggaag agagcgccac ggacagtatg tcacaggtta
    13561 aaagtgcagc cacgcagacc tttgatggta ttgcacagaa tatggcggcg atgctgaccg
    13621 gcagtgaaca gaactggcgc agcttcaccc gctccgtgct gtccatgatg acagaaattc
    13681 tgcttaagca ggcaatggtg gggattgtcg ggagtatcgg cagcgccatt ggcggggctg
    13741 ttggtggcgg cgcatccgcg tcaggtggta cagccattca ggccgctgcg gcgaaattcc
    13801 attttgcaac cgggggattt acgggaaccg gcggcaaata tgagccagcg gggattgttc
    13861 accgtggtga atttgtcttc acgaaggagg caaccagccg gattggcgtg gggaatctct
    13921 accggctgat gcgcggctat gcggaaggtg gttatgtggg cggtgccgga agtccggcgc
    13981 agatgcggcg ggctgaaggc attaatttta atcagaacaa tcacgtggtg attcagaacg
    14041 acggtacgaa tggtctgcca ggtccacaga tgatgaaggc cgtgtatgac atggcccgca
    14101 agggtgcccg tgatgaaatt cagacacaga tgcgtgatgg tggcctgttc tccggaggtg
    14161 gacgatgaaa accttccgct ggaaagtgaa acccggtatg gatgtggctt cggccccttc
    14221 tgtaagaaag gtgcgctttg gtgatggcta ttcccagcga gcgcctgccg ggctgaatgc
    14281 caacctgaaa acgtacagcg tgacgctttc tgtcccccgt gaggaggcca tggtactgga
    14341 gtcgtttctg gaagagcacg ggggctggaa agcctttctg tggacgccgc cttatgagtg
    14401 gcggcagata aaggtgacct gcgcaaaatg gtcgtcgcgg gtcagtatgc tgcgtgttga
    14461 gttcagcgca gagtttgaac aggtggtgaa ctgatgcagg atatccggca ggaaacactg
    14521 aatgaatgca cccgtgcgga gcagtcggcc agcgtggtgc tctgggaaat cgatctgaca
    14581 gaggtcggtg gagaacgtta ttttttctgt aatgagcaga acgaaaaagg tgagccggtc
    14641 acctggcagg ggcgacagta tcagccgtat cccattcagg ggagtggttt tgaactgaat
    14701 ggcaaaggca ccagtacgcg ccccacgctg atggtttcta acctgtacgg tatggtcacc
    14761 gggatggcgg aagatctaca gagtctggtc ggcggaacgg tggtccggcg taaggtttac
    14821 gcccgttttc tggatgcggt gaacttcgtc aacggaaaca gtgacgccga tccggagcag
    14881 gaggtgatca gccgctggcg cattgagcag tgcagcgaac tgagcgcggt gagtgcctct
    14941 tttgtactgt ccacgccgac ggaaacagat ggcgctgttt ttccgggacg tatcatgctg
    15001 gccaacacct gcacctggac ctatcgcggc gatgagtgcg gttatcacgg tccggcggtc
    15061 gcggatgaat atgaccagcc aacgtccgac atcacgaagg ataaatgcag caaatgcctg
    15121 agtggctgta agtttcgcaa taacgtcggc aactttggcg gcttcctttc cattaacaaa
    15181 ctttcgcagt aaatcccatg acagagacag aatcagcgat tctggcgcac gcccggcgat
    15241 gtgctccagc ggagtcgtgc ggcttcgtgg taagaacgcc ggagggggaa agatatttcc
    15301 cctgcgtgaa tatctccggt gagccggagg cgtatttccg tatgtcgccg gaagactggc
    15361 tgcaggcaga gatgcagggt gagattgtgg cactggtcca cagccacccc ggtggtctgc
    15421 cctggctgag tgaggccgac cggcggctgc aggtgcagag tgatttgccg tggtggctgg
    15481 tctgccgggg ggcgattcac aagttccgct gtgtgccaca tcttaccggg cggcgctttg
    15541 agcacggggt gacggactgt tacacgctgt tccgggatgc ttaccatctg gcggggattg
    15601 agatgccgga ttttcatcgt gaggatgact ggtggcgtca cggtcagaat ctctatctgg
    15661 ataatctgga ggccacaggg ctgtatcagg tgccgttgtc agcggcgcag ccgggcgatg
    15721 tgctgctgtg ctgttttggt tcatcggtgc cgaatcatgc cgccatttac tgtggtgacg
    15781 gcgagctgct gcaccatatt cctgaacaac tgagcaaacg agagaggtat accgacaaat
    15841 ggcagcgacg cacacactcc ctctggcgtc accgggcatg gcacgcatct gcctttacgg
    15901 ggatttgcaa cgatttggcc gccgcatcga ccttcgtgtg aaaacgggtg ccgaagccat
    15961 ccgggcgatg gccacacagc tccctgcgtt tcgtcagaag ctgagcgacg gctggtatca
    16021 ggtgcgtatt gccgggcagg atgttagcac gtccggatta acggcgcagt tacatgaggc
    16081 tctgcctgac ggcgctgtga ttcatattgt tcccagagtc gccggggcca agtcaggtgg
    16141 tgtattccag attgtcctgg gagcagccgc cattgccgga tcattcttta ccgccggagc
    16201 cacccttgca gcatgggggg ctgccattgg tgccggtggt atgaccggca ttctgttttc
    16261 tctcggtgcc agtatggtgc tcggcggtgt ggctcagatg ctggcaccga aagccagaac
    16321 tccccgcaca cagacaacgg ataacggtaa gcagaacatc tatttctcct cactggataa
    16381 catggttgcc cagggcaatg ttatgcctgt tctgtatggt gaaatgcgcg tggggtcacg
    16441 tgtggtatct caggagatca gcacggcaga cgaaggggac ggtggtcagg ttgtggtgat
    16501 tggtcgctga tgcaaaatgt tttatgtgaa atcgcctccg ggcggtttta tcgtttatgg
    16561 agcatgacga atgggtaaag gcagcagtaa ggggcatacc ccgcgcgaag cgaaggacaa
    16621 cctgaagtcc acgcagctgc tgagtgtgat cgatgccatc agcgaagggc cggttgaagg
    16681 tccggtggat ggattaaaaa gcgtgctgct gaacagtacg ccggtgctgg acagtgaggg
    16741 gaataccaac atctccggcg tcacggtggt gttccgggca ggtgagcagg agcagtcacc
    16801 gccggaggga tttgaatcct ccggctccga gacggtgctg ggtacggaag tgaaatatga
    16861 cacgccgatc acccggacca tcacgtcggc aaacattgac cgactgcgtt ttaccttcgg
    16921 tgtacaggca ctggtggaaa ccacctcaaa gggagacagg aatccgtctg aagttcgcct
    16981 gctggttcag atacagcgta acggtggctg ggtgacggaa aaagacatca ccattaaggg
    17041 caaaaccacc tcgcagtatc tggcctcggt ggtggtggat aacctgccgc cgcgcccgtt
    17101 taatatccgg atgcgcagaa tgacgccgga cagcaccaca gaccagctgc agaacaaaac
    17161 gctctggtcg tcatacaccg aaatcatcga tgtgaaacag tgctacccga acacggcact
    17221 ggtcggcgta caggtggatt cggagcagtt cggcagccag caggtgagcc gtaattatca
    17281 tcttcgcggg cgcattctgc aggtgccgtc gaactataat ccgcagacgc ggcaatacag
    17341 cggtatctgg gacggaacgt taaaaccggc atacagcaac aacatggcct ggtgtctgtg
    17401 ggatatgctg acccatccgc gctatggcat ggggaaacgt cttggtgcgg cagatgtgga
    17461 taaatgggcg ctgtatgtca tcggccagta ctgtgaccag tcggtgccgg acggctttgg
    17521 cagcacggag ccgcgcatca cctgtaatgc ctggctgacc acacagcgca aggcgtggga
    17581 tgtgctcagt gatttctgct cggcgatgcg ctgtatgccg gtatggaacg ggcagacgct
    17641 gacgttcgtg caggaccgac agtcggataa ggtgtgggcc tataaccgca gtaatgtggt
    17701 gatgccggat gatggcgcgc cgttccgcta cagtttcagc gccctgaaag accgccataa
    17761 tgccgttgag gtgaactgga ttgacccgga taacggctgg gagacggcga cagagcttgt
    17821 ggaggatacg caggccattg tccgttacgg tcgtaacgtc acgaagatgg atgcctttgg
    17881 ctgtaccagc cgggggcagg cacaccgcgc cgggctgtgg ctgattaaaa cggaactgct
    17941 ggagacgcag accgtggatt tcagcgtggg tgctgaaggg cttcgtcatg taccgggcga
    18001 tgtcattgaa atctgcgatg atgactatgc cggtatccgc accggcgggc gcgtgctggc
    18061 ggtgaacagc cagacccgga cgctgacgct cgaccgtgaa atcacgctgc catcctccgg
    18121 caccacgctg ataagcctgg ttgacgggca ggggaatccg gtcagcgtgg aggtccagtc
    18181 cgtcaccgac ggcgtgaagg taaaagtgag ccgtgttcct gacggtgttg ctgaatacag
    18241 cgtgtggggg ctgaagctgc cgacgctgca ccagcgattg ttccgctgcg tgagtatccg
    18301 tgagaacgac gacggcacgt atgccatcac cgccgtgcag catgtgccgg aaaaagaagc
    18361 catcgtggat aacggggcgc actttgacgg cgaccagagc ggcacggtga atggtgtcac
    18421 gccgccagcg gtgcagcacc tgactgccga agtcaccgca gacagcgggg aatatcaggt
    18481 gctggcgcgc tgggacacgc cgaaggtggt gaagggcgtg agcttcctgc tccgtctgac
    18541 cgtaacagcg gatgacggca gtgagcggct ggtcagcacg gcccggacga cggaaaccac
    18601 ttaccgcttc acacaactgg cgctggggaa ctacaggctg acagtccggg cagtaaatgc
    18661 gtgggggcag cagggcgatc cggcgtcggt atcgttccgg attgccgcac cggcagcgct
    18721 gtcgcggatt gagctgacgc cgggctattt tcagataacc gccacgccgc atcttgcggt
    18781 ttatga
//
LOCUS       DAESHI010000082        18513 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.083, whole genome shotgun sequence.
ACCESSION   DAESHI010000082 DAESHI010000000
VERSION     DAESHI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18513
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.083"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            76..288
                     /gene="cspH"
                     /locus_tag="KW011_002916"
     CDS             76..288
                     /gene="cspH"
                     /locus_tag="KW011_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HBH7661203.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            735..1040
                     /locus_tag="KW011_002917"
     CDS             735..1040
                     /locus_tag="KW011_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661204.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            1147..1791
                     /gene="gfcB"
                     /locus_tag="KW011_002918"
     CDS             1147..1791
                     /gene="gfcB"
                     /locus_tag="KW011_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HBH7661205.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1788..2534
                     /locus_tag="KW011_002919"
     CDS             1788..2534
                     /locus_tag="KW011_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBH7661206.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2534..4630
                     /locus_tag="KW011_002920"
     CDS             2534..4630
                     /locus_tag="KW011_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBH7661207.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSQLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4676..5815
                     /locus_tag="KW011_002921"
     CDS             4676..5815
                     /locus_tag="KW011_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HBH7661208.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5803..6249
                     /gene="etp"
                     /locus_tag="KW011_002922"
                     /gene_synonym="yccY"
     CDS             5803..6249
                     /gene="etp"
                     /locus_tag="KW011_002922"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HBH7661209.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            6269..8449
                     /gene="etk"
                     /locus_tag="KW011_002923"
     CDS             6269..8449
                     /gene="etk"
                     /locus_tag="KW011_002923"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HBH7661210.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8564..9862)
                     /gene="appA"
                     /locus_tag="KW011_002924"
     CDS             complement(8564..9862)
                     /gene="appA"
                     /locus_tag="KW011_002924"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HBH7661211.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9942..10034)
                     /gene="cbdX"
                     /locus_tag="KW011_002925"
     CDS             complement(9942..10034)
                     /gene="cbdX"
                     /locus_tag="KW011_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HBH7661212.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(10047..11183)
                     /gene="appB"
                     /locus_tag="KW011_002926"
     CDS             complement(10047..11183)
                     /gene="appB"
                     /locus_tag="KW011_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBH7661213.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(11195..12739)
                     /gene="appC"
                     /locus_tag="KW011_002927"
     CDS             complement(11195..12739)
                     /gene="appC"
                     /locus_tag="KW011_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HBH7661214.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12873..13730)
                     /locus_tag="KW011_002928"
     CDS             complement(12873..13730)
                     /locus_tag="KW011_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HBH7661215.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRKRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13787..14125)
                     /gene="hyaE"
                     /locus_tag="KW011_002929"
     CDS             complement(13787..14125)
                     /gene="hyaE"
                     /locus_tag="KW011_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HBH7661216.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            complement(14122..14709)
                     /locus_tag="KW011_002930"
     CDS             complement(14122..14709)
                     /locus_tag="KW011_002930"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HBH7661217.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGRLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14706..15412)
                     /gene="hyaC"
                     /locus_tag="KW011_002931"
                     /pseudo
     CDS             complement(14706..15412)
                     /gene="hyaC"
                     /locus_tag="KW011_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            complement(15431..17224)
                     /gene="hyaB"
                     /locus_tag="KW011_002932"
     CDS             complement(15431..17224)
                     /gene="hyaB"
                     /locus_tag="KW011_002932"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HBH7661218.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(17221..18339)
                     /gene="hyaA"
                     /locus_tag="KW011_002933"
     CDS             complement(17221..18339)
                     /gene="hyaA"
                     /locus_tag="KW011_002933"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HBH7661219.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
BASE COUNT         4546 a         4914 c         4782 g         4271 t
ORIGIN      
        1 gcggtcttaa atatcgctgg ctttatttta ttaaccccgt atagtgcagc gcagtatttt
       61 cagtaaggaa tttgtttgtc ccgtaaaatg acaggaattg tcaaaacctt tgatcgcaaa
      121 agcggcaaag gattcattat cccctccgac ggtcgtaaag aagttcaggt ccatatttcc
      181 gcattcactc cccgcgacgc agaagtgctt ataccaggat tacgtgtgga attttgccgc
      241 gtaaatggcc tgcgaggacc aacagcggca aatgtttatc tctcttaata tgacgctgct
      301 tgtttaaagc tgactggttt tattggaata gtgcaatagt caacatcata cattataaca
      361 tcagttactt ttccacaaag aaactaatcc atttaagcga atatctctgc acctaatagt
      421 tataaatgcc atttaacagc cagttatagt accacttgaa actatattac caacaataat
      481 taatagcttt cgtaacaatt tccttatctt taatttgtat tttgctaaga tttatcttat
      541 gctcacaagt cgtatttcca gaggggatcg ttaataatct tatatttccc tctgcgcacc
      601 tttcctaccc gcagtatcag tatatagtgc aagcggtatg aggaaaggga gatttcacaa
      661 ggatgttata ctcaaagtag acaatcctga tctcaagcgt acgtattgtc catgcgatga
      721 ataaaggaaa agttatgaaa cacaagcttt ctgcaatcct tatggccttc atgttgacga
      781 cgccagctgc gtttgctgcc tctgaagcaa caaacgggac cgaggcaacg acgggtacta
      841 ctggcaccac aacgaccact accggcgcaa ccacgactgc tactaccact ggtggcgtgg
      901 ctgctggcgc tgtcggcacg gcgacagttg tcggtgtggc aacggctgtt ggtgtggcga
      961 cgttggcagt tgttgcagca aatgacagtg gcgatggcgg ttctcataac acctcaacca
     1021 ccacgtccac tacacgctaa tactgcttta acgcgtcgaa attaaagtta aatgataccc
     1081 ggttgcgata ttcgtaccgg gctttagaga tttgcatttt tttaattaac ctttcaagga
     1141 ttaaaggtgc gccctcttat tttatcgatt ttcgcactat ttcttgcggg atgtacgcac
     1201 agccagcaaa gtatggtcga tacatttcgc gccagccttt tcgataatca ggatatcacc
     1261 gtagcggatc agcagatcca ggcgttgcct tattccacga tgtatttacg ccttaatgaa
     1321 gggcaacgaa tctttgtggt actgggatat atagaacaag aacaaagcaa atggttatcc
     1381 caggataacg ccatgctggt tacccacaat ggacgtcttt taaaaaccgt caaacttaat
     1441 aataatctgc tggaagtgac aaattccggg caggaccctc tgcgtaacgc gctggcaata
     1501 aaagatggca gccgctggac gcgcgatatt ctctggagtg aagacaacca ttttcgctct
     1561 gcgaccctga gttctacttt ttcctttgct ggattagaga cgctgaatat tgcgggtcgc
     1621 aatgtgctgt gtaatgtctg gcaggaagag gtgacttcca cgcggccaga aaaacagtgg
     1681 caaaacacat tctgggtcga ttcggctact ggccaggttc gtcaaagtcg gcaaatgtta
     1741 ggcgcagggg ttattcccgt agaaatgacg tttcttaaac ccgcaccatg aataaattac
     1801 agtcgtattt cattgccagc gtactttacg taatgacacc ccatgccttt gcgcaaggaa
     1861 cggtgactat ttatctgcct ggcgaacaac agacactttc cgttggcccc atggaaaatg
     1921 ttgtccagtt ggtgacacaa ccgcaactgc gcgatcgcct ctggtggcct ggtgccttgc
     1981 tgaccgactc tgcggcgaaa gctaaagcgc ttaaagacta ccaacatgtc atggcacagc
     2041 ttgcttcctg ggaagcagaa gctgacgacg atgtggcggc aacgattaaa tccgtgcgcc
     2101 agcagttact caatctcaat atcactggtc gcttaccggt caaactggac cctgattttg
     2161 tacgagtgga tgagaacagc aatcccccgt tggtcgggga ctatacgctc tataccgtac
     2221 agcgtccggt gactattact ctgctgggtg cggtttctgg ggccgggcaa ttgccctggc
     2281 aggcaggccg tagcgttacc gattatctgc aagatcaccc ccgccttgca ggcgcggaca
     2341 agaataacgt tatggtgatt acgcctgagg gtgagactgt cgtcgcccct gttgccctgt
     2401 ggaataaaag acatgtggaa ccaccgcctg gcagccagct ttggttgggc ttttctgcgc
     2461 atgtactgcc cgaaaaatat gctgatttaa atgaccagat tgtgtctgtt ttaacgcaac
     2521 gtgttcctga ctgatgaaga agaattctta tcttttaagc tgcctggcca ttgccgtctc
     2581 cagtgcctgt catgctgaag tattaaccta cccggatccg ctgggttcgt cgcaatcaga
     2641 ctttggcggc acaggattgt tgcagatgcc aaatgcgcgc atcgcaccgg aaggtgaatt
     2701 cagcgtcaac taccgggata acgatcaata ccggttctac tccacctccg tggcgctgtt
     2761 cccatggctg gaaggcacca ttcgttatac ggatgtgcgc acacgcaaat atagccagtg
     2821 ggaagatttc agcggcgatc agtcatacaa agacaaatca ttcgatttta aacttcgcct
     2881 gtgggaagaa ggttactggc taccgcaagt ggcgtttggt aaacgtgata ttgctggtac
     2941 gggtctgttt gacggtgagt atctggtggc cagcaagcaa gcggggccat ttgatttcac
     3001 cctcgggatg gcatggggct acgccggtaa tgcgggcaat attaccaacc cgttttgccg
     3061 ggtgagcgat aaatattgtc atcgcgcaga gtctcacgat gcgggcgata tcagctttag
     3121 cgatatcttt cgtggcccgg cttccatctt tggcggcatt gagtatcaaa cgccgtggaa
     3181 tcccctgcgt ctgaaactcg aatacgatgg caacaattac cagaatgatt tcgctggcaa
     3241 actgcctcag gcaagccatt tcaacgtcgg cgcagtttat cgcgctgcca gctgggcaga
     3301 tctcaacctg agttatgaac gcggtaacac gttgatgttt ggcttcacgt tacggaccaa
     3361 tttcaacgat ctgcgccctg ccctgcgcga tacgccaaaa ccggcatatc aacctgcgcc
     3421 tgaatctgaa ggattgcagt acaccacagt agcgaaccaa cttaccgcgc tgaagtacaa
     3481 cgcaggtttt gaagcaccgg aaattcagct gcgcgataag acgctgtata tgtctggtca
     3541 acaatacaaa taccgtgatt ctcgcgaagc ggtcgatcgt gccaaccgga ttctggtgaa
     3601 taacctgccg caaggcgttg agaagattag cgtgacgcaa aagcgcgagc atatggcgat
     3661 ggtgactacc gaaaccgacg tagccagcct gcgcaaacag ctggctggta cagcgcctgg
     3721 tcaatcagag caacttcaac aacaacgtgt tgaagcagaa gatctttctg cctttggtcg
     3781 gggctaccgt attcgtgaag atcgctttag ctactctttc aacccaacac tttcacagtc
     3841 gctgggcggc ccggaagatt tctatatgtt ccagctgggg ctgatgtcca gtgcccgcta
     3901 ctggtttacc gaccacctgc tgcttgatgg cggtattttc accaatattt acaacaacta
     3961 cgacaagttt aagtcttcgc agttgcccgc ggactctacc ctgccccgcg tgcgcacgca
     4021 tatccgtgat tacgttcgca atgacgttta tctcaacaac ttgcaggcga actactttgc
     4081 cgacttaggc aatggtttct atggccaggt gtatggcggt tatctggaaa cgatgtacgc
     4141 cggtgtcggt tccgagctgc tttatcgccc gctagatgcc agctgggcgc tgggtgtgga
     4201 cgttaactac gtgaagcaac gtgactggga caacatgatg cgcttcaccg attattccac
     4261 gccaactggt ttcgtgacgg cttactggaa cccgccgacg ctcaatggcg tactgatgaa
     4321 acttagcgtt gggcaatatc tggcaaaaga taaaggggca acgatcgacg tcgccaaacg
     4381 ctttgacagc ggcgtggcgg taggggtatg ggcggcaatc agtaacgtat ctaaagatga
     4441 ctacggcgaa ggcggcttta gtaaaggttt ttatatctcg attccgttcg acttgatgac
     4501 cattggacct aaccgcaacc gcgcggtggt ttcgtggaca ccattgacgc gtgatggtgg
     4561 acaaatgctg tcacgcaaat accagctcta tccaatgacg gcagagcgag aagtaccggt
     4621 tggacaataa cctgacgtaa ttcataacat tacattgact gataaatata aaccaatgaa
     4681 aaagaacatt tttaagtttt cggtactgac attggctgtt ctgtctttga cagcatgtac
     4741 ccttgttcct ggtcagaatt tatcgaccag taacaaggat gtcattgaac tacctgacaa
     4801 tcagtatgac ctggacaaaa tggtcaatat ctatcccgtc acgccaggat tgattgatca
     4861 gttgcgcgcc aaaccgatta tgtcgcaggc taacccggag ctggagcagc aaatcgcaaa
     4921 ctatgaatat cgtatcggga ttggtgatgt gttgatggtt acggtctggg atcacccgga
     4981 actaaccacc cccgcagggc aataccgtag cgccagtgat accggcaact gggttaacgc
     5041 cgatggtgcg attttctacc cctatattgg tcgcctgaaa gtggccggga aaacacttac
     5101 ccaggtacgc aacgagatta cagcgcgtct cgattcagtc attgaaagcc cgcaagtcga
     5161 tgtcagcgtc gcggcgttcc gatcgcaaaa agcgtacgtc accggcgaag tcagcaaatc
     5221 gggtcagcag ccgattacca atattcctct gaccatcatg gatgcgatta acgccgcggg
     5281 cggtctgacc gcagatgcag actggcgcaa cgtggtgctc acccagaatg gcgtaaagac
     5341 caaagtgaac ctctatgcgc tgatgcagcg tggcgacttg cgccagaaca aactgctgca
     5401 cccgggcgat attttattta ttccgcgcaa cgatgatctg aaagtcttcg tgatgggtga
     5461 agtgggtaaa cagagcacgc tgaaaatgga ccgcagcggt atgaccctgg cggaagcgct
     5521 gggtaacgct gagggtatga atcaggatgt cgctgatgcg acggggattt ttgttattcg
     5581 tgcaacgcaa aacaagcaga atggcaaaat cgccaatatt tatcagctga atgcgaagga
     5641 cgcatcggca atgatactcg gaacggaatt ccagcttgaa ccttacgaca ttgtttatgt
     5701 cactaccgcg ccgctggctc gttggaaccg tgtgatttct ctgctggtac cgacgatttc
     5761 cggggtgcat gacctgacag agacttcacg gtggattcaa acatggccca actaaaattt
     5821 aactcaatcc tggtggtttg taccggcaac atttgccgtt cgcccattgg cgagcgcttg
     5881 ctgcgtaaac gtctgccggg cgtaaaagtc aaatcggcag gtgttcatgg tctgataaaa
     5941 caccctgcag atgcgacagc ggcagatgtt gccgctaatc atggcgtatc tcttgaagga
     6001 catgccggac gtaagctcac tgctgagatg gcacgaaatt acgatctgat tctggcaatg
     6061 gagtcggaac atattgctca ggttaccgca attgctcccg aggtccgcgg gaagacaatg
     6121 ctgtttgggc aatggctgga acagaaagag atcccggatc cctatcgtaa aagtcaggac
     6181 gcatttgaac atgtctacgg tatgttggag cgcgccagtc aggaatgggc gaagcgcctc
     6241 agccgataat ttgagtttat aaaatacgat gacaactaaa aatatgaata cgccaccagg
     6301 cagcactcag gaaaatgaga tcgatctgct tcgtctggtc ggcgagttat gggatcaccg
     6361 taagtttatt atcagcgtga ccgcgttatt cacgctgatc gctgtcgctt actcgctgtt
     6421 aagcacacca atttatcagg cagatactct ggtccaggtt gagcaaaaac agggcaacgc
     6481 cattctcagc ggcctgagcg atatgatccc taactcatcg cccgagtctg caccggagat
     6541 ccaactgctg caatcgcgca tgattctcgg taaaaccatt gctgaactga atctgcgcga
     6601 catagttgag cagaagtatt ttccgattgt gggtcgcggc tgggcgagat taaccaaaga
     6661 aaaaccaggt gagctggcga tcagctggat gcatattcca caactgaatg gtcaggatca
     6721 gcaactgaca ctcacggttg gggaaaacgg ccactataca ctggaaggtg aagagttcac
     6781 cgtcaatggt atggtcggcc agcgtctgga aaaagatggc gttgcgctga ctatcgcgga
     6841 cattaaggcc aaaccaggaa cacagtttgt cctgagccag cgtaccgaac tggaagcgat
     6901 taacgcattg caggaaacct ttaccgttag cgaacgcagt aaagaaagcg ggatgctgga
     6961 acttaccatg actggtgatg atccccagtt gattactcgt attctgaaca gcatcgctaa
     7021 caactatttg caacagaata tcgctcgcca ggcggcgcag gattcacaaa gccttgaatt
     7081 cttacagcgc cagttacctg aagtgcgcag cgagctggac caggcggaag aaaaactcaa
     7141 cgtttatcgc cagcagcgcg attcggttga ccttaacctg gaagccaaag ccgttcttga
     7201 gcagattgtg aacgttgata atcaactcaa tgagctgacc ttccgcgagg cagagatctc
     7261 ccagctgtat aagaaagatc acccaactta tcgtgcgctg ctggaaaaac gccagacgct
     7321 ggagcaagaa cgcaaacgcc tgaataagcg ggtatcggca atgccttcca cccaacagga
     7381 agtgttgcgt ttaagtcgtg acgtagaagc gggccgtgcg gtatatctgc aattacttaa
     7441 ccgccagcag gagttgagta tttcgaaatc cagtgccatt ggtaacgtgc ggattatcga
     7501 cccggcagtc actcagccgc agccagtgaa accgaaaaaa gcgttgaatg tggtgcttgg
     7561 ttttattctt ggcctgttta tttctgtggg tgccgtgctg gcgcgtgcga tgttgcgtcg
     7621 tggtgtagaa gccccggaac aactggaaga gcacggcatc agcgtttatg ccactatccc
     7681 aatgtccgag tggctggata aacgcacccg tctgcgtaag aaaaatttat tttctaatca
     7741 gcagcgccat cgtactaaaa atatcccctt cctggcggtg gataacccgg cggattctgc
     7801 tgtggaagcc gtacgtgcgc tacgaaccag tctgcatttc gctatgatgg agacggagaa
     7861 taacattctg atgatcaccg gtgcgacgcc agacagtggt aaaacgtttg tcagttcaac
     7921 tctggcagcg gtgatcgccc agtccgatca aaaagtgtta tttattgatg ccgacttacg
     7981 ccgtggttat tcgcataacc tgtttaccgt gagtaatgaa catggcttgt cggaatatct
     8041 ggcaggtaaa gatgagctca acaaagtgat ccagcatttt ggcaaaggag gctttgatgt
     8101 gattactcgc ggtcaggtgc cacctaaccc gtctgaactg ctgatgcgcg atcggatgcg
     8161 tcaattactg gaatgggcga acgaccatta cgatctggtg attgtcgata cgccgccgat
     8221 gctggcggtg agtgatgccg cggtcgtggg gcgttctgtt ggcaccagcc tgctggttgc
     8281 gcgttttggc ttgaacaccg ccaaagaggt gagtttgtca atgcagcgtc tggaacaggc
     8341 aggcgtcaat attaaaggcg ctatcctcaa tggtgtgatt aaacgcgcca gcaccgctta
     8401 cagttacggc tataactatt acggttatag ttactccgag aaagagtaaa actgaaagtg
     8461 gccggatgcg gcgtgaacgc cttttccggt ctacgcatta gacagttctt cgtttgtcat
     8521 cagcctcaga gcattcaggt aactgaatgc tcttttttat gcattacaaa ctgcacgccg
     8581 gtatgcgtgc ttcattcacg atttgcgtaa aacctgccaa cgaacacatg ccctgcgcat
     8641 ttcgctcttc acatcctgcc agggtcagtt tcacctctcc gggcggcgta tttaatgaca
     8701 gcggcgtttt atcacgcatc tgctgtaaag tctggaagac cagcgaaacc tgaatccact
     8761 ggctgttatc gcttagccga cgccagcgtt caaacaccag ttcaccacct ggcggcgtgt
     8821 tatccggctg accgggaagc gtccagttga gctccagtgc gccgccgaga tttgccagat
     8881 tagtatcgtg tccggcgata aacagcactg aagtgggtaa tgtcacacca tacgcctgtt
     8941 tttgcggtgg atggggcgtc aacgctgtct tgatcaaatc taataacggg gtggcgcggc
     9001 tgcgggcaac ctctggcgtg cgttgtagca aataaaattg cgcgttatgc aaacttagca
     9061 aggtgttcca ctggtgtgaa tcggtgatcc ttccccaccc cggctccggc attccctgtg
     9121 cttgttgcag gagaaatatc tccgtcagca ttgatgcgag gcttaccgca ccggttaatg
     9181 agacattgtc ggcgctcacc ttgagttccg atggtaatgc ctgcgttaat gaacagcttt
     9241 cgtcctgttt ctcacgttta aggcacaagt ttgattgcgg aaaattaagc acccgttcca
     9301 gttcgcgaaa cgccgtttgc cgatgcccgg taaagtcagc aattgaccct cctgccctgc
     9361 tgaggatcgc gtcagtcacg ttcgcgttat ccagttggca aacgccagtt tttagaggat
     9421 taaataacgg atcgggactg gacgtatcag cctgggtatg tacggttatt gcacagtcag
     9481 gtgccagccc ggcggcgaag gcttcgcctg ttttacgggt acgctcgtcg acatcagcaa
     9541 taatcgcgac ctgaccagac tgcgggcagc cctttttcgc cagcaatccg tcggctacca
     9601 gacgctggcg ttggtaatgt ccgagatagg cgattagctc accaccgcgc ggtgtcagcc
     9661 aacccagttt taccggccag gttggccatg cgtctggggt gacatcctgc atcagttgcg
     9721 tggccttggt tggagcacgc acaccatgac gactgacaat caccacactt tccagcttca
     9781 gctccggctc actctgagcg aatgcagatt gcggggttaa cggaatcaga agagataaaa
     9841 atgggattaa gatcgctttc atcgatatgt ttccgctttt catatctgac attattgatt
     9901 gcctgatgcg atgctaatgc gccgcatcag gcctacgttc gttaactttt cagacgcggg
     9961 tccagccata ccaacacaag ggtggagagc gaacacatca acaaaatgcc gacgaaccaa
    10021 agtaaatacc acattgttcc tgctccttag tacaactcgt tttcgttacg gcggagagtt
    10081 tctgttgtca tgcgtcccca cattttgtag tagctccaga gagtgtagag caacacaatg
    10141 ggcaaaaata tcagcacgat taccaacata atgctcagcg tcagctgact ggaagtgctg
    10201 tcccacaacg tcaggctgga gattggactc acgcttgacg gcatgacaaa ggggaacagc
    10261 gtgatgcctg ccgtgaaaat cacgccaaat tgcatcaatg acgccattaa aaatccccaa
    10321 cccgggcgac cacgataaat cgccatcacc gtcagcaatg ggcagaagaa ccccagcagc
    10381 gggaagatcc acaaaacggg cgattcgacg aaattattca tccaggcacc aggtagcact
    10441 gccaccagtt tcattaacgg attggaagga ccgttagcat cctgggcgag cagtacaaag
    10501 ccatcaatac cgacccacag ccagtaaccc gccagcaaaa agcacagcat caccaacagt
    10561 gcagcgcgtt tggtggccag ctgtgaacgc agatgaatca caccaacagt tttcagttgt
    10621 aaccagacgc caccttgcaa aatcaccatc ccaaggctga gcaatccgca caataaaggg
    10681 aatggcgtca gcagttgcca gaagctaccg agatactcca cgcgtaattg cggtgtgaag
    10741 gcaaacggca cgccgagcaa caagttgccg aaggcgatac cgaagactac cggcggcacc
    10801 agactgccga tgaccagacc ggcgtcccac attttacgcc agcgtgcatc ggcgattttt
    10861 ccgcgataat caaaggccag cgggcggaag aacagtgagc acagcaccag gatcatcgcc
    10921 acataaaagc cggaaaacgc cgctgcatac actctgggcc aggcggcaaa taatgcccca
    10981 ccagcgagga tcaaccagac ctggttgcct tcccagtgtg caccaacgct gtttatcact
    11041 atccggcgtt catcatcatt acgcgccacc agcggcagca gacagccgat ccccatgtca
    11101 aatccgtcgg agatcataaa gaccaccagg atcacgccaa tcagcagcca ccagatgaag
    11161 cgcaatgttt cataatcaaa catgattttc ccctttaccc ctgttgctgc gtcggttgtt
    11221 cactctgcat cgcgctcggc cccagacggg catatttctg catcaggtag acttcggcga
    11281 ttaagaacag ggtgtaaagc cctacgatca tgatcagtga gaaagccagt tgtcctgtgg
    11341 ttaaagcgga gtgcgcggag tatgtcggta agatgtcctg tatcgcccac ggctgacgac
    11401 caaactcggt cataaaccac ccggcttcaa tcgcaatcca cggcaacggc aaactccaga
    11461 gcgccatttt cagcacccag cgatgctggt cgattttgcc acgcagcgtc tggacaagcg
    11521 caatcagcat caccagtagc ggcagggaac cacagcccac catgatgcgg aaactccaga
    11581 ataccggcgc aacctgaggt atcgcgccac gcatcgccgc ctggtactgt gcggctgtga
    11641 catgattcat atccggcgca tagcgggaga gcaacatgcc gtagcccagg tcaccttcca
    11701 gttcccggaa tgcctgcaac acatgcggct cacgattgcc ttgcgatatt tcctgcatta
    11761 acagccaggc catacgtccg cgttgcaagc gtgggtaggt ttcagccatc aaatttttca
    11821 gacccggcac ggttttatct aatgagtgag tggcgaggat ccctaacagc gcgggaattt
    11881 tgatggcaaa ggcgttacgc tcttgatcct gttccggcca ggcaaccaca tggaacggtg
    11941 caggagcagg ttccgtttgc cactcccctt ccatcgccgc cagttttacc ggttgtactt
    12001 gcgcgacttc atacgcagaa ctgtctccga gttgtagcgt gccgataatc gccagagtac
    12061 cgaagacgga accgatggca aacgagcgta atgcaacatc gcgctcccgt ccgcgcagta
    12121 aataccaggc gctgatcgcc ataataaaca tggccccggt cacgtagccc gccattacgg
    12181 tgtgcacaaa tttcacctgg ctgaccgggt taaagaccag ctcgctgaag ctggtcatct
    12241 ccatacgtag ggtgtcgata tcaaaatgcg caccggtcgg gtattgcatc caaccgttgg
    12301 cattcaatat ccacaatgca gagagatttg aaccgaacgc caccagccac gtcaccagca
    12361 ggtgctggta tttattcagc cgctgccagc cgaagaagaa aagcccgaca aaggtggatt
    12421 cgaggaagaa ggccattaat gcttccatcg ccagcggtgc gccaaaaata tcgcccacat
    12481 agttggaata gaatgaccag ttagtaccaa actgaaactc catggtcagg ccggtagcca
    12541 cgccaagagc aaagttgata ccgaagagct taccccagaa gcgcgtcata tcgcggtaga
    12601 ttgttttgcc ggtgaccacg taaatggttt ccatgatagc cagcaaaaaa atcagcccca
    12661 gggtaagggg tacaaataaa aagtgataca gcgcggtcag agcaaactgc cagcgcgata
    12721 aatcaatgac atcccacatg cgcactcctg taggcgaaga gagaaaaata acccgataat
    12781 gttagatctc cctctttatc ctgaaactct acgaccagag gtattcgccg attgcgatat
    12841 tcataagact gaaagcgata cttaccgtct ttttacgtcg gtgcagcttc cgccagccac
    12901 tgacatacct cgctaagccg ctgcgcagag tcgaccaaat cttctggcgc tgccagcacc
    12961 acttccggta ttgggcagat ttcataactt tccagtaaag gaccttttaa ggtgtccgta
    13021 cagcgtaaat gccagacatg gcgtaacccc gtggcgttga tatagctctc gccatagcca
    13081 atggtacgaa tctgaatatt tcccggccca cagagacgtg agagaaaaag ccgatcagcc
    13141 tcgctgatgg gtaattgcgt cagattaatg ctgtgcggct gcgcgtcggg gttacgtaca
    13201 tgcgccagta actcatgcgc caacggtagg ccattcatca ggccatcaat gggcggcggt
    13261 aacagcgaat ctgtcggcaa gagattttgc gtggccgcct gccacaacgc cagcggcgcg
    13321 cagccggcct ccagtttgtc ctccagcaac ttttcgccgc gacgtttgcg cacccgccat
    13381 aatccgcaga agatcgcctc ctgtatttca ctttcgctgt cgtcagcctg ctgaatgcgc
    13441 actgacactt ccccttcccc gagaagcgta ttgataaagt gacgatcgtc ggcgttgaga
    13501 tttaacagat cgactgccag cacctcgccc aacggcggct ggcgttgact tagttgatgt
    13561 tgtaactcgt ttaacagcgc aatcacctgc gggctgtgag cacattgctc cagggccgcc
    13621 atactcggtt catcattcac ctgacagctg atcggcagtg gattcatgct gaaactgtca
    13681 tcgttcggtt gcgttcctgg ccccagcaga tggaaaaaag tttcgctcat gaggcacgct
    13741 cctgttgtgg ttcgacaagc ccacgcatca ggtttatcag ttccgctcac gggtgaatac
    13801 cattcagcac accgcgatag tttccgccgg taaacactaa tgtggcggga aagcgaaaga
    13861 cgccaaagcg atctccgatg gcttcgctct gttcaaggtc agcaatcgcc acctgccatg
    13921 tgtagccggg aaactcgcgc agtaattcgc caatcattac cggattatcg ctgacctctg
    13981 gcgtgcgttt cgggtcactg cttaataaca ccacgccgtc tggcgcttgc gtaagccagt
    14041 cgtcaagacg gaattcacta accggcgtcc agccgcgcgc cagcattcgt tgccacaacg
    14101 catcaaatgg cgtgtcgttg ctcatccctg ctcctccagt gtcatcctgt actggcgcaa
    14161 gcgaacgcct tcgtaatttt ccatcgacag acagtcataa ttgagacagc gcgattcatt
    14221 agccggttgc ggcacaattc cccatgcagc cagctgcgcc agcgccgcct gttccgctgc
    14281 gggcagttgc tcccgtgcca gttcgctcag gcggccgccg tagtcgtcga gcattgcggg
    14341 ttgcagaccg acgagggcaa tatgtgctgg cagatgtccg cggatatctg ccagcgccag
    14401 cacttcggag aaactgttct ggtgcaggct cattttcttc gcgctgagat aagccggaat
    14461 acgttctccg gcataggttc gcagcgttcc aggttccagc ccgtagtcaa tggcatcgag
    14521 aatcaacaga tggctggcgc tttcgacata ccccagcaag ttcagtccct gagtaccgcc
    14581 atcgacaatc tccacatact cgggccagtg gtaatgggca tacaaccgtt ccgccacccg
    14641 cacgccgaaa ccttcatcgg cccacagcag gttgcccagc cccattacca caacgcgttg
    14701 ctcgctcatg aacgctcctt gttacttatt ttgccaaatt tgtggctacg gtagccgttg
    14761 accatggtgg agatcaccgt gtcgtcggac atgatgtctt cacgcagcgc catatagaca
    14821 tgaccgatca caaacgcgcc aatcagccac atccccagcc gatgccagct gtgaatgtcc
    14881 attgagttgc ctcccgtcca gtagaaaaat tccaccacat aacggaacgg cgcaaaaata
    14941 gcgtactggc tgtgttcgct gtacagcgca aagccggtga tgatcataaa gaccgacatc
    15001 aggaaatagc cgaacatcgc cgcctgggcg atggggttat ggcctatatc ggcactcgga
    15061 cgttttgcca gaacagatac cagcggattt cataccacac gccctgccac cagcttttac
    15121 gccataccgg cacgataaac agctcgcggg agtatcgatt gccaacaaaa gcccagtaga
    15181 tccgcatcag caagaccacg gtaaaaacca tcccggcgct gaagtgaatt aacctgatgt
    15241 agcccatata gaacagatac gtcgcctcgc cgctgacgga aggtagcggc ttgccgataa
    15301 agtatccggt gaccatcaac accgccatgc ataacaccgt cagccagtgc cagatgcgga
    15361 ctggcgcttc aaagacatag tggctgacaa cgttgtcgct tttctgttgc atgatgattc
    15421 tccttcgctg ttaacgcacc tgcacggaga tcagctcgct accgtcgtcg cccagcacgt
    15481 gtgttgaaca ggcgaggcac gggtcaaagc tgtgcagagt acgcaggatc tccagcggtt
    15541 gctcggggat cgccattttg gtgttcatca gcgccgcttc ataagcgcca atctgccctt
    15601 tgggatcgcg cgggctggcg ttccaggtgg tcggcaccac gcactggtag agatcaatct
    15661 tgccatcgcg aatggcggcc cagtggccta acgccccgcg cggcgcttcg gtaaagccga
    15721 cgccacggca ctctgtcggc caggttgcag gttcccattt ttccgtggag gcggtggcaa
    15781 gattgccgtt tttcaggtta gtcatcagct tgttgaagaa atattgcaac ttacctgcgg
    15841 cccactgtgc ttcgtgcgcg cggcacaaaa tgcggcccaa cgttgactgg ataccggaaa
    15901 gcggcaggtt cagcgccgac atcatgcgat cgaccgactc aacggtcgca gcatcgcctt
    15961 tgtgataagc gattaacgtg cgtgccagcg gccccacttc catcgcgtta ccgcgccagc
    16021 gcggcgcttt gatccacgag tagcgttcct gttcattcag ctgctgaatg ttggtatcgc
    16081 tgcctttgac atcgccgggg ttgtaccacg ggtcggtaat gccatcgaat ggatgacgcc
    16141 cgacctgatc gttgggataa cgataccagg cgtggtcgac aaactcctgc acctgctgcg
    16201 gatcaaccaa atccactggc agcacattgt tgaagtcgcc gttaatcacc gcgccgccag
    16261 gcatcagcag acttttctca ccaaagtcgt tggcaatatc cgggaacgca ccgtagctga
    16321 ggacacattt atcagaaaga cccgtgccga tttcgctcca cggtttgttg aactgaccga
    16381 tggctaaggc gtcggggatc atcacgttgt taatgaagtc cgccgtgcgg gtgataatcg
    16441 actgcaccag gttcaggcgt tccatattga ccgccccgac cgcgccgctt tcgtcaatgt
    16501 taatggcgca aggcatcccg ccgacaatcc agtttggatg cgggttttta ccgccaaaga
    16561 ccgcgtggat tttgacaatt tcacgctgga aatcgagagc ttcgagatag tgggcaaagc
    16621 ccatcaggtt agcttctggc ggcagtttgt actgcgggtg cccccagtag ccattgcgga
    16681 agatccccaa ctgcccgcct tcaacaaatt ttttcaggcg gttttgtacg tcgaagaaat
    16741 agccagggga tgatttcggc caagaggaga gactttgcgc cagttcggag gttttccgcg
    16801 ggtcggcttt cagcgcatct aacacatcga tccagtccat cccggcaagc tgatagaagt
    16861 gcaccagatg atcgtggcac cagagcgttg ccagcataat gttgcggatg atattggcgt
    16921 tgtccggcac tttaataccg atagcatctt cgatggcgta aaccgaagcc agggcgtgta
    16981 cgccagtaca gacgccgcag atacgttcaa cgaacgccca cgcatcgcgc gggtcgcgcc
    17041 cttgtaggat gatctccagc ccgcgaaaca tggtgccgca ggagacggca ttggtgatca
    17101 cattctgatc gttaatattc acttcgcagc gcatgtggcc ttcgatgcgc gtaatcgggt
    17161 cgaccaccag gcggcgtccg gcattattga tggtgtatcc ctgagtttcg tactgagtgc
    17221 tcatgcctgt ttatcctcat tgcctggctg atgttcggtt tctgtaggtt gctggttatg
    17281 acgtctgcgc tggtcaacgg cgctggcgac tgcgtgcaca ccaacagccg ctgccaccac
    17341 accaagcgcg gttaaaccga cggtatcggc ggtggaatga gtacccattt gcggaatatc
    17401 gaccacgcgg ctgtagaacg aaccgcaatc ccagaaacca ttttccgcac agcccaggca
    17461 gccgtgacca gactggattg ggaaagaaac gccatcattc cagcgtgtgg aggaacaggc
    17521 gttataggtg gtaggccctt tgcagcccat tttgtacagg cagtaacctt tgcgggcagc
    17581 gtcatcatcc caactctgga cgaactctcc ggcgtcgaag tgggcgcggc gatagcattt
    17641 atcgtggatt cgctgaccat agaacatcag cggacggccc attctgtcga catctggcaa
    17701 gcgatcaaag gtcaccatgt aagtaatgat ggcgctcatc acatccggga tcggcgggca
    17761 gccaggtact ttgataatgg gtttgtcggt gatgactttg tcgataggcg ttgcctgcgt
    17821 cggattgggc cacgcggcct gcacgcagcc ccaggacgcg caggttcccc aggcgataat
    17881 cgcgctggct ccggcagcgg cacgtttgag tttctcaata aacggtcgac cgctgctgat
    17941 acagaacatc ccctgctcgc ccagcggcgg attaccttct actgcgagga tatatttgcc
    18001 attgtattgc gtgatgatgt cttcgaagac ttcttccgcc tgggttccgg cggcagccat
    18061 caaagtatcg tcataatcga gggaaatcag ggaaaggatg acgtccttcg ccagtgggtg
    18121 agcggagcgg ataaaagatt cggtacagca ggtgcattcc agaccgtgga tccataccac
    18181 cggaatgcgg ggtttgttct ccagcgccca ggcaatcttt ggtgccattc ccgcgcctaa
    18241 tcccagcgac gtggcagcca gactacaata tttgagaaag ctgcgccggg taacgccctg
    18301 acgccgcatg gcctggtaaa atgtttcctc gttattcata tcgcacgtct cttctccttg
    18361 cgacaccggc aggacaccgg gcgcttatgg tcggcaagcg acgtccctgt agcaaggcaa
    18421 accatcgctt cacgtgagat gctgaaaacg aaagctcatc cttctgcact ggcgcacgtc
    18481 gccagaaagt attgttaata aagcgtagtg aaa
//
LOCUS       DAESHI010000083        18211 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.084, whole genome shotgun sequence.
ACCESSION   DAESHI010000083 DAESHI010000000
VERSION     DAESHI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.084"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            15..1091
                     /locus_tag="KW011_002934"
     CDS             15..1091
                     /locus_tag="KW011_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HBH7661220.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRAN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKIYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1484..1825)
                     /locus_tag="KW011_002935"
     CDS             complement(1484..1825)
                     /locus_tag="KW011_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HBH7661221.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1838..2710)
                     /gene="copD"
                     /locus_tag="KW011_002936"
     CDS             complement(1838..2710)
                     /gene="copD"
                     /locus_tag="KW011_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HBH7661222.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="KW011_002937"
     CDS             complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="KW011_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HBH7661223.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3227..3457
                     /gene="holE"
                     /locus_tag="KW011_002938"
     CDS             3227..3457
                     /gene="holE"
                     /locus_tag="KW011_002938"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HBH7661224.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3559..4215
                     /locus_tag="KW011_002939"
     CDS             3559..4215
                     /locus_tag="KW011_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBH7661225.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4239..4901
                     /gene="exoX"
                     /locus_tag="KW011_002940"
     CDS             4239..4901
                     /gene="exoX"
                     /locus_tag="KW011_002940"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HBH7661226.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="KW011_002941"
     CDS             complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="KW011_002941"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HBH7661227.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7167..7826)
                     /locus_tag="KW011_002942"
     CDS             complement(7167..7826)
                     /locus_tag="KW011_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HBH7661228.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(8153..8509)
                     /locus_tag="KW011_002943"
     CDS             complement(8153..8509)
                     /locus_tag="KW011_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HBH7661229.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="KW011_002944"
     CDS             complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="KW011_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HBH7661230.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            9000..10178
                     /gene="purT"
                     /locus_tag="KW011_002945"
     CDS             9000..10178
                     /gene="purT"
                     /locus_tag="KW011_002945"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HBH7661231.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="KW011_002946"
                     /gene_synonym="eda"
     CDS             complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="KW011_002946"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBH7661232.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10912..12723)
                     /gene="edd"
                     /locus_tag="KW011_002947"
     CDS             complement(10912..12723)
                     /gene="edd"
                     /locus_tag="KW011_002947"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HBH7661233.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="KW011_002948"
     CDS             complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="KW011_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBH7661234.1"
                     /translation="MAVTQTTQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14771..15640
                     /gene="hexR"
                     /locus_tag="KW011_002949"
                     /gene_synonym="yebK"
     CDS             14771..15640
                     /gene="hexR"
                     /locus_tag="KW011_002949"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HBH7661235.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15768..17210
                     /gene="pyk"
                     /locus_tag="KW011_002950"
     CDS             15768..17210
                     /gene="pyk"
                     /locus_tag="KW011_002950"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBH7661236.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17270..18112)
                     /locus_tag="KW011_002951"
     CDS             complement(17270..18112)
                     /locus_tag="KW011_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBH7661237.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
BASE COUNT         4668 a         4573 c         4393 g         4577 t
ORIGIN      
        1 ggagaagtta aaacatgcag cggaaaaaat acgatcccaa tttgcctaga aacttaacat
       61 atcgaaggag agacaaagca tattactggc gcaaccctct gacgaaagaa gaatttacac
      121 taggtaaaat ttcaagaaga gatgcagtaa cgcaggcaat tgaagcaaat cattatatat
      181 acaaaaacta ctctcccgct gccttaattg aaaaacttaa agggttcgac tcatttacta
      241 tggcagactg gattgaacgt tacaaaacga ttcttataag gagaaaagtg tccagaaata
      301 cttataaaat tcgggcaaat caactggaga caataaaaga aaaattggga gagattttac
      361 tgacagaaat aaccactcgc catattgccg agtttcttga tttgtggatt gaaggaggga
      421 aaaacacaat ggcaggatca atgcgttctg tgttgtctga tatgtttcgc gaggccattg
      481 ttgaaggacg tatatctcaa aatccagtca cgccaacaag agcaccgaaa atagtagtta
      541 caagagaacg actgaaacta aagatataca actgcatcag ggaggcagca gatcaacttc
      601 cggcatggtt cccattagct atggatttag cccttgtaac aggacaacgt cgcgaagaca
      661 taacgaatat gcggttcagt gatatttatg atgatcgtct ccacgtcagg caaattaaga
      721 caggaatgat gattgctatc cccctgtcac tcagccttcc tgtcgctggt ctacggcttg
      781 gtacagtagt tgaacgatgc cgcctggtaa gccggggaga ttatctaatc agtgccggga
      841 ttagaaaaaa cagccctgac ggcagcattc acccggatgg cctgacaaaa aaatttgtcg
      901 cagccagaaa attcacaggt atccagttca gtgaaaaccc accaactttt cacgagatca
      961 gaagcctggc tggacgattg tacaaagaaa catgtggaga agaatttgct cagcgtctac
     1021 ttggccacac atcggagaag acaacaaaaa tgtatcttga tgagagagaa aaaacgtact
     1081 tactgctctg attttaacgt aaatggattg ttaaatgtat tttggttgtg atataaccaa
     1141 aaagaccgga atacagaaat tcgagtaaat ttcggggaat ttcgggggag acatttgcaa
     1201 ctaattgatt tcaaatgcaa ttaaaaaaag accgaatacg attcctgtat tcggtccagg
     1261 gaaatggctc ttgggagaga gccgtgcgct aaaagttggc attaatgcag gcttagttgc
     1321 cttgcccttt aagaatagat gacgacgcca ggttttccag tttgcgtgca aaatggtcaa
     1381 taaaaagcgc ggtggtcatc agcttaaatg ttaaaaaccg cccgttctgg tgaaagaact
     1441 gaggcggttt ttttattgga aatcaaaagg ctattttagg taattaacag agtttttcag
     1501 ctcgttctat aaacggtgcc agactcattt tttcgccggg attgttagga tcatcaatct
     1561 gaatcaccga aatgggttgg gcattggtct tcccactggc aacttccttt tgtgcgatat
     1621 cgtttaaagg atactgcacg agggtactcg gattaataac atacaaagca ttacccggtc
     1681 ggcaagtcag catcacctct tcgcgattaa acgcccattt gtctttaccc acttcaaaac
     1741 gactgacggt aatcacctgc ggtgcagcca gcgccgctgc agaactggtg agtaacagaa
     1801 acgccagaat actttttttc atcattttcg taatccatca aaaaggttcg agagtcgcaa
     1861 gcaggctgat gatcaccagc actaccgcac ctatcgccca ttcgagttta gtcatccaca
     1921 caaaccaggg tgccgcacga tcttcctcct gcctcatgcg cggtacaaga acataacgat
     1981 tagccagcgc aatgaccacc ataatcatca ccaggatggc tttgagcaat aagagttggc
     2041 cccagtaagt cgtaagcgtg gggggaaatc cggtaatcaa caatgcatta agcacgccgc
     2101 ttgccagtac gccgatcacc gcaaaatgcc cgcaccatga aaaacacatc agcgcctgaa
     2161 tagcctggtg tcgccaacgg cctttgatga gttgcataca ccagagcacc ggcagtaatc
     2221 ccccaaacca cgccgccgca caaatcaggt gaatcgcgtg attagtctga tggattttcg
     2281 ccgttacccc ttcattcagt gtcgcatgcc ccattcccgc cagcagaata aattgcgcgg
     2341 tagtgagcat aaacagcaaa cgtggcatat tccgcggttg cataagggcg acgatcaacg
     2401 ttacgagggc gagaacaatt tgccataacc agacgccacc aaactgcgtt tgcaaaacag
     2461 cctgccaaat gtttggcgaa aatacatctg tccatcccgt tcccatcaac ccgccctgaa
     2521 cggcgagcat tgcagtggca ctgatcaaac tccagacggc agcgtgctgt tgtaaacgca
     2581 gaaaacgctt cgccagcaag cgacgaatcg tcaagggggc cagccaggca ccgtacattg
     2641 cgaagccaaa aaccagcatc agcgaggtga aatggataaa tcgcagcgcg atccaggtaa
     2701 acgccagcat gatttatttc acgctaaagg tgtagtgtcc tttcgtttta tgcccgtcca
     2761 ccgaaacaac atgccagtca acggtgtagg ttccgggttt cagcgaatcg gccagtggaa
     2821 caatcagttg cttttggtcc tgctcatttc gcttcgccgg taatgtttta atattttcgt
     2881 tttttggccc cgtgattttt gcaccactga atcctgtttc cacaccttcc gagaagttta
     2941 aagtgattgc ctgcggtgca gctgtcactt gcgcgtttgc cgcaggatac tgatgcgtta
     3001 aatgcgcatg tgcccagacg aaaggtgtca ccaacgaagt ggtcaggatt gccagcgcgt
     3061 agcgaaggga gcgtgcagtt gaagccatat tatctattcc tttttgtaat aactttttta
     3121 cagagcataa ccttgtctaa tgtctgagtc gaggatcatc aattccggct tgccatcctg
     3181 gctcactctt agtaactttt gcccgcgaat gatgaggaga ttaagaatgc tgaagaatct
     3241 ggctaaactg gatcaaacag aaatggataa agtgaatgtc gatttggcgg cggccggggt
     3301 ggcatttaaa gaacgctaca atatgccggt gatcgctgaa gcggttgaac gtgaacagcc
     3361 tgaacatttg cgcagctggt ttcgcgagcg gcttattgcc caccgtttgg cttcggtcaa
     3421 tctgtcacgt ttaccttacg agcccaaact taaataaaac ttatacaggg ttacactttc
     3481 ttacataacg cctgctaaat tatgagtatt ttctaaaccg cacccataat ttgcagtcat
     3541 tttgaaaagg aagtcattat gtcgttctgg aaagttgcag cagcgcaata tgagcctcgt
     3601 aaaacctcac tcaccgaaca agtagctcat catctggagt ttgtgagggc cgccgccagg
     3661 caacaatgtc agctgttagt ttttccatcg ctttctttac tggggtgtga ttattcgcga
     3721 cgtgcccttc ctgccccacc cgatctctca ctgttagacc cgctttgtta tgccgcaacg
     3781 acctggcgaa tgaccatcat tgccggcctt cccgttgaat ataacgatcg ctttattcgt
     3841 ggaattgcgg tgttcgcacc gtggcgaaaa acgcctggga tctaccatca gagtcatggt
     3901 gcatgtctgg gccgtcgttc cagaaccatc acggtggtcg atgaacaacc ggaaggtatg
     3961 gatatggacc cgacctgttc actgtttacg acagggcaat gtctgggaga acccgacctg
     4021 ctggcgtcgg cccgtcgctt acaatttttt tcacatcagt actcgattgc tgtgctgatg
     4081 gcaaatgccc gtggtaacag tgcactatgg gatgaatatg gtcgtctcat cgttcgcgcc
     4141 gatcgtggtt cattattgtt agtcggtcag cgttcatcac agggttggca aggcgatatc
     4201 attccattac gctaggcttt ttcggcctgg agtatgccat gttgcgcatt atcgatacag
     4261 aaacctgcgg tttgcaggga gggatcgttg agattgcctc tgttgatgtc attgacggaa
     4321 aaatcgtcaa ccccatgagc cacctggtgc gccccgatcg tcctattagt ccacaagcga
     4381 tggcgattca tcgcatcacc gaagccatgg tcgccgataa accgtggatt gaagatgtga
     4441 tcccacacta ttacggcagt gaatggtatg tcgcgcacaa cgccagcttt gaccgccgcg
     4501 tactgcctga gatgcccggt gagtggattt gcaccatgaa actggcccgt cgcttgtggc
     4561 caggaatcaa gtacagcaat atggcgttat ataaaacacg gaagctcaat gtacagacgc
     4621 cgccgggcct gcatcatcac cgcgcgttgt atgactgtta tatcaccgcc gcgttgctta
     4681 tcgatattat gaacacctcc ggctggacgg cagaacagat ggccgatatc accggacgtc
     4741 cgtcgttgat gacgaccttc acctttggca aataccgtgg caaagcggtt tccgacgttg
     4801 ccgaacgcga tccgggctat ctgcgctggt tatttaataa cctggacagc atgagcccgg
     4861 agctgcgttt aacactgaaa cattatctgg aaaatactta atccgcaggc gtagcgggta
     4921 atgttccctg cgccagcgcg accagaaaag catattccat cgctacgcct tcgtacgatt
     4981 taaagcggcc agatttaccg ccatggcctg agtccatgtc ggtacagagc aataaaagat
     5041 ggttatcggt tttcagctcg cgcaatttag cgacccattt tgccggttcc caatattgca
     5101 cctgagaatc gtgtaaaccg gtcgttacca gtaaatgcgg ataagcctgt gcggtgacgt
     5161 tgtcatacgg gctgtagctt ttcatgtact cgtaatattg cggatcctgc gggttacccc
     5221 actcttcaaa ctcaccagtg gtaagaggaa ttgattcatc aagcatcgtt gttacaacat
     5281 caacaaacgg tacctgggcg ataacgccgt ggaataattc cgggcgttga ttaattgcaa
     5341 cgcccatcaa catgcccccc gcactcccgc ccatcgcata acaaagcgaa ggagagccat
     5401 agcccagttt taacaatgca tcgcaggcat caagataatc attaaacgta tttttcttct
     5461 tcagaaattt tccgtcttcg taccattgtt gccccagctc accaccgccg cgaacatgga
     5521 caatggcgta gacaaagcca cgatctaaca aactcaagcg gctaaaactg aaatcggcat
     5581 caatacttgc gccgtaagaa ccatagccat acaccagcaa cgggttgtgt cctttgcgaa
     5641 aatgtttgcg atggtagacc aacgaaaccg gaacttcgac gccatcacgg gcgactatcc
     5701 acaggtgttc actgcggtaa ttcgccgcat caaaaccagg aacttccgtt tgttttaata
     5761 cacgacgctc atcggtatcc atatccagtt caaacaaagt gtctggtgtg gtcatggaag
     5821 aataaccata acgcaatcgc gcggtttcag gttctggatt gtaggcaatc caggtcacat
     5881 aggctggatc atcaaaggca ataccaatga cttcccgggt cttgcggtta atttggcgca
     5941 aactggttaa cccgcgctga cgctcttcaa ccaccagcca gtcggtaaac agcgtaaacc
     6001 cttccagcat gatgttttcg catggcggaa ttaactcttc ccactgttgc tcatcacgca
     6061 tacgggtacg gtataagcca aagtttttgc cgtggcggtt ggaacgcaga taaaaacgat
     6121 gctggtagtg atcaaggctg tattcgtgat ctttgcggcg cggcagaaaa acaaacggct
     6181 cggcatcggc catttccgcg tccagcaggc gaacttcact ggtggtggcg ctggccaaat
     6241 gaatgactac atagtgcttc gaggtcgttt tatgcaggct gacgtaatag gtatcgtctt
     6301 tttcttcgta gatcagttta tcttgcgatg ctggcgtacc gatggcgtga cgccagacct
     6361 gataaggcag cagcgtcacc ggatgcttgc gaacatagta gaaagtccag gagtcatttg
     6421 cccagacaaa gctgggttca acgttatcca gcagttccgg gtaccagtta ccagtttcca
     6481 gattacgaaa acgaatgccg tactggcgtc gggaaagaaa atcttctgcc agcgccataa
     6541 tggtgttatc gggcgtaatc gccattccgc ccatcgaata aaactcacta tgagctgcgc
     6601 gcttattggc atcgagcaat atttcccact catcccactc ttcactgaat gccgattgac
     6661 gctggtagat agcatattca cagcctggtt cataaatatg ccgatagcgg tagccatttt
     6721 tgatgtaggg cgcagaaact tctcgttgcg gaatgcggtc gatgatttcc tttaagatgc
     6781 gatcctgcaa ggcttgttgt gaggccatca cccgatgacc gtaactattt tcttgttgca
     6841 ggtagtccag gacttctggc tgagaacgcg tatcatcccg cagccagtag taattatcga
     6901 tgcgcgtatc gccatgaagc gtcatggcgt ggggaatgcg ggcggctttt ggtagcatgt
     6961 tattgttctt tctggttgaa acatcttata agggtggcaa aactcaccgg ggatgcaagc
     7021 gaaacagggg agtcattgct tagatgatga caggtaatgg cacggatatc gaatgttatg
     7081 cagacagaga aactcagcct gttcaaatgg ctgtgcggtt ctggatagcc cgaaatagtc
     7141 aacttcaggc tatccagaga gcggaattat tccgccaaag tgcgtttttg ctgttcgaga
     7201 tcgcgttcaa tgccgtcacg aacatcctgg gggattttca gcgcgtcacc cagtgcattc
     7261 aggtaactgc gttccataaa atggtcaata tcaatagccg cgcaactcag gaaatagatt
     7321 tccagcgcct cttcttcatt gcggaccccg gtagccaggc gttgtggatc cagcggttgt
     7381 tcgattgcct gctcaatgag tacacgcccc tgctcttcca cgccggcttc acgcaattgc
     7441 tggtcgattg ccgcacgttc tttggcatca atatgaccat cacttttagc ggcaaagacc
     7501 agcgcaagga tcaaacgttc tgtacgctca tccagcggcg tactttgcgc gccaaactgc
     7561 ggttcgtcct gatgcgccgc gcgaatttta tctttgtatt tattccacag caccgtaccc
     7621 gctaccgctc cgccgccaac cagtaacgcg ttggtgccat atttcgtaag aagtttacgt
     7681 gctgatttat ttgcgaccag cagcccagcc agtccgccta atgcgcctgg cactaacagt
     7741 ttgcccaatc cctgatccgc agacgaggag gtagaagaac tgctttgccc aagcagggat
     7801 tgcagttgat ttaaccagtt agccatattt gctcctcaat aaccattttt agtgcctgta
     7861 gcatagcgga tgaggatgtc agaaaatgta tatcgagaca aaagatgcgc aaataagcgg
     7921 ggcggtaggc cgttattcaa agaaattcgc gccaggtaag gtatttactg tatatcaggt
     7981 gataagggta ttttaggtga aagtagtttg tcgttttgag tggcattgtt tgatgctgat
     8041 tgcccgatgc gacgctgttg cgtcttctca ggcctacgat tgtggtgcgt tctggggcgg
     8101 acaaggcact cgcgccgcat ccaccaacgg tcaatcgaat tatcagaagc cattaacgcc
     8161 gctgatattc cgccattccc gctttgcagt ccacgctgac ctggtaatga atatcggcac
     8221 ttttaccacg cacggttagc ggtactgacc atttatcatc tttaccctga atgtcctgca
     8281 aactgaccca agccacggga tcggcctgac cgacaatttt ttgatcatct gcccaacgcg
     8341 ccacgcgatt ttgttgataa tcacgtttta cgctcgcggc aattccggca gcatccagac
     8401 cttcacactt tgggaaagtg accgacttgc tggtttcatt attggcagcg aaaactgatg
     8461 cgcaggcaga aaccaacaac agccctaaaa acgcccctct ttttttcatg tttttctcca
     8521 tagcacaatg attcaggaga aagcatggta caaattgtca ggagcgcaag ttgcttcagg
     8581 ccgcgtgtga ggcatcttcc tcttcggatt cagcaccgac gacctgtggg gacggtaatt
     8641 tgccggtttt cagaatggtg ctcagcacat ctttttgttc tgccagccat agcgaaaggg
     8701 cttcacgttg ctcgtcttcc atttgcactg gagaatttgt cagccaggtg tcaagcagat
     8761 ccgccgtatc aagcattttg tcataagcat cggcttcctt tttgctggta aacgacattt
     8821 tctcttcgcc ctcacgaatg actacgtatt taacttcaac cgccatttgc agcctctcat
     8881 aataactgtg attttataca gtatatttct tttcggttga gaaatcaaca tcagcaataa
     8941 agacacacgc aaacgttttc gtttatactg cgcgcggaat taatcagggg atattcgtta
     9001 tgacgttatt aggcactgcg ctgcgtccgg cagcaactcg cgtgatgtta ttaggctccg
     9061 gtgaactggg taaagaagtg gcaatcgagt gtcagcgtct cggcgtagag gtgattgccg
     9121 tcgatcgcta tgccgacgca ccagccatgc atgtcgcgca tcgctcccat gtcattaata
     9181 tgcttgatgg tgatgcatta cgccgtgtgg ttgaactgga aaaaccacat tatatcgtgc
     9241 cggagatcga agctattgcc accgatatgc tgatccaact tgaagaggaa ggactgaatg
     9301 ttgtcccctg cgctcgcgca acgaaattaa cgatgaatcg cgagggtatc cgtcgcctgg
     9361 cggcagaaga gctgcagctg cccacttcca cttatcgttt tgccgatagc gaaagccttt
     9421 tccgcgaggc ggttgctgac attggctatc cctgcattgt aaaaccggtg atgagctctt
     9481 ccggcaaggg gcagacgttt attcgttctg cagagcaact tgctcaggca tggaagtacg
     9541 ctcagcaagg cggtcgcgcc ggagcgggcc gcgtaattgt tgaaggcgtc gttaagtttg
     9601 acttcgaaat taccttgcta accgtcagcg cggtggatgg cgtccatttc tgtgcaccgg
     9661 taggtcatcg ccaggaagat ggcgactacc gtgaatcctg gcaaccacag caaatgagcc
     9721 cgcttgccct tgaacgtgcg caggagattg cccgtaaagt ggtgctggca ctgggcggtt
     9781 atggtctgtt tggtgtcgag ctgtttgtct gtggtgatga ggtgattttc agtgaggtct
     9841 cccctcgtcc acatgatacc gggatggtga cgttaatttc tcaagatctt tcagagtttg
     9901 ccctgcatgt acgtgccttc ctcggacttc cggttggcgg gatccgtcag tatggtcctg
     9961 cagcttctgc cgttattctg ccacaactga ccagtcagaa tgtcacgttt gataatgtgc
    10021 agaatgccgt aggcgcagat ttgcagattc gtttatttgg taagccggaa attgatggca
    10081 gccgtcgtct gggtgttgcg ctggcgaccg ctgagagtgt tgttgacgcc attgaacgcg
    10141 cgaagcacgc cgccggacag gtaaaagtac agggttaaac ccggcaaaaa aacgctacaa
    10201 aaatgcccga tcgtagatcg ggcgttttga cttttacagc ttagcgcctt ctacagcttc
    10261 acgcgccagc ttagtaatgc ggtcgtaatc gcccgcttcc agcgcatctg ccggaaccag
    10321 ccaggaacca ccgatgcaca gcacgctttt cagcgccagg tagtcacggt agttagccgg
    10381 agaaatacca cccgtcgggc agaaacggac ctgggagaac ggacccgcga tcgcctgcag
    10441 ggctttcacg ccgccgttag cttcagccgg gaagaatttg aactctttca aaccgtagtc
    10501 catacccagc atcagttcgg aaacagtgct gatccccgga attagaggaa tagtcccttc
    10561 ggtagcagct ttcagcagcg gctcggtcag acccgggcta attgcgaact gtgcacccgc
    10621 ttcagtgact tctgccagct gctgtggatt cagcaccgta ccggcaccca caatcgcttc
    10681 aggcacttct ttggcgatag cacggatagc gtcaactgca cactcggtac gcagagtcac
    10741 ttccagaacg cgcaccccac cagcaaccaa cgcttttgcc atcggcactg cgtgttccag
    10801 tttttttacc acgataaccg gtacaaccgg gccggtggtc aggattgatt ctgcacttgt
    10861 tttccagttt ttcatcagag ttttctctcg cctgattaca aatttgtcgt cttaaaaagt
    10921 gatacaggtt gcgccctgtt cggcaccgga cagtttttca cgcaaggcgc tgaataattc
    10981 acgtcctgtc cccacgcgtg acgcgctcag gtcaggaatg tgcggttcgc gagcagccag
    11041 ttccgcttcg tctaccagca gcgtcagttc gcctgtctgt ccattcacac gaatgatgtc
    11101 cccgtcgcgc acttttgcca gcagcccgcc atcgtaggct tctggtgtta cgtggatagc
    11161 tgacggcact ttacctgaag cgccggagag tcgtccatcg gtaactaacg caattttgaa
    11221 acaccggtcc aataatacac caagtggcgg catgagttta tgtaattctg gcattccgtt
    11281 cgcttttggc ccctgatgac ggacaacaac gacacaatcg cggtccagca aacccgcttc
    11341 aaaggccggc ataacgtcat gctggctttc aaaaacaacc gctggcgctt caatcacctg
    11401 gttctcaacc ggcacggcag aggttttcat aaccgcacgg cccaggttac cgcttaacac
    11461 ttttgtccca ccatgatgag agaaaggttg ttcgaaggaa gcgatcacat tgctgtcgag
    11521 tgatttttcc gccccttccc gccagtcctg ttcaccatta ttcagccatg gttcaagggt
    11581 ataacgagac agaccaaagc ctgccaccgt attgacatct tcatgcagca ggcctgcttt
    11641 gagcagttca cgcaccagaa ccggtacgcc acctgccgcc tggaagtggt taatatcggc
    11701 cggaccgttc gggtagagac gtgccatcag cggtacaaca tcagaaaggt cagagaagtc
    11761 atcccagtta atctgaatac cggccgcgcg cgccatcgcc accaggtgca tggtgtggtt
    11821 agtggaacca ccggtcgcca gcagtgcaac gataccgttc accaccactt tctcatcgat
    11881 catcttaccg atcggcatcc attcattacc attaccggtc atgcgtgtaa cctgacgcgc
    11941 agcggcagcg gtcaaagcat cgcgcagcgg agaatccgga tgaacaaaag aagagcctgg
    12001 caactgcatc cccataaatt ccaccaccat ctggttggtg ttggcagtac cgtagaaagt
    12061 acatgttccc ggcgcatggt aagacgcggc ttctgactcc agtaaggcca tgcggtccac
    12121 tttaccttcg gcataaagct ggcgaatacg cactttttct ttatttggca aaccgcttgc
    12181 catcggtcca gacggcacaa acaccgcagg caaatgacca agcgacaggg ctgccatcgt
    12241 cagacccggg acaatcttgt cgcacacacc gaggaacaga gcaccatcaa acatgttatg
    12301 ggacagcccc accgccgcag acatcgctat cacttcgcgg cttagcagcg acaattccat
    12361 tccatcctgc ccctgggtga caccatcaca catcgccgga acaccgcccg caacctgacc
    12421 aaccgcattc gcttcatgca gggctttacg aatgatttct ggatagtgtt cataaggctg
    12481 gtgcgcggag agcatgtcgt tataggaggt gatgatggcg atattgttac gcaacatgct
    12541 tttcaaagag gctttgtctt ctggctggca ggcagcgaaa ccgtgtgcca ggttaccgca
    12601 tgccaactgc gaacgatgaa cggtcgacgt tttcgcttgt tctatccggg cgagataagc
    12661 agagcgagtc tcgcgcgaac gttcaatgat tcgatttgtt acgcataaca attgtggatt
    12721 cataaaggct cctgaaattg agttgtcaga gcaggatgat tcacaacgcg tttcattcag
    12781 aggatttatg agtgaaacgc ctgtaaccgg agctcatagg gcaaaaacgt ttcagtcagt
    12841 gtaataaaaa aagcctcgtg ggtgaatccg cacgaggcct gaaagtgtaa aaattgttct
    12901 acaatctgtg caagatcatg ttaccggtaa aataaccata aaggataagc gcagatatta
    12961 ctcaaactca ttccaggaac gaccatcacg ggtaatcatc gccaccgagg caacgggtcc
    13021 ccaggttccg gcctgatacg gtttcggcgc atcattgtcc atcgcccacg cctcagtaat
    13081 ggagtctacc catttccagg cttcttccac ttcatcacga cgtacaaaca gtgcctgaat
    13141 accacgcatg gtttccagca gcagacgttc ataggcatcc gccagatgcg tctgattaaa
    13201 ggtttctgaa tagctcagat ccagcttggt gatttgcagg ttatgtttgt ggtcaaggcc
    13261 aggaacttta ttcagtacct ggatatccac gccttcatca ggttgcagac ggatagtcag
    13321 tttattctgc ggcagatcct gccacgactc tttaaacaga ttcagttcag gtgttttgaa
    13381 atagaccacg acttcagaac atttggtcgg cagacgttta ccagtacgca ggtagaatgg
    13441 cacaccggcc cagcgccagt tatcaatgtc gacgcggatc gccacgaagg tttctgtatt
    13501 gctgctcttg ttcgcgccct cttcttccag atatcccggc acttttttgc cctgggcgaa
    13561 gcccgcagta tattgcccgc gtacggtttt ttcgcgtacg ttggagcggt cgatgcggcg
    13621 cagggacttc agtactttca ctttttcatc gcggatgctg tctgcgctca ggtcagatgg
    13681 cggagacatc gcaatcatgc agagaatttg tagcaggtgg ttctgaatca tgtcacgcat
    13741 ctgaccggct ttatcaaaat agccccagcg cccttcgatc cccacttctt ctgccacggt
    13801 aatctcaaca tgatcaatgg tgcgattgtc ccagttattc acaaacaggg agttagcaaa
    13861 acgcagcgcc aacaggttca gcaccgtttc tttaccaaga tagtggtcga tacggtaaac
    13921 ctggcactcc tcgaagtatt cgccaacctg atcattgatt tcctgcgagg tcgccagcga
    13981 cgtccccagc ggtttctcca tgactacgcg tgccggttta gcattcagtt ttgcctcgcc
    14041 aagccctttg caaattgcgc caaaagtgct gggcggcatg gcaaagtagt taatggtgat
    14101 acgatttttt tgatccagca tcgcgccgag acggttgaat gcagcagtgt cattgacatc
    14161 gagattacaa aaatccagac gtgcactcag ggtgtcccat aaaccttcat caatggtttc
    14221 tttcatgaaa gtttcgagcg cctcgcggac aactttggta tatgccgctt tatcccagtc
    14281 agcacgccct acgccgataa tccgggtgtc cgggttgagc tgaccggctt tttccagttg
    14341 atacagggaa ggcagcaatt tacgacgcgc aaggtcgcct ttcgcgccga aaatgaccag
    14401 gtcacaggcc tgggttgttt gcgttaccgc catgtcattc tccttaagtt aactaacccg
    14461 gtacttaagc cagggtatac ttgtaatttt cttacggtgc actgtactgc ttttacgagc
    14521 ttgcgaaaac tgtaaacgct tatccacccg tgcgattacg ggaaaagcgc gcaaagtgcg
    14581 gcaaaactga taaaaaaatc atcgttttcc attcggtaaa aatctgacac tgatcatgtt
    14641 atgaaaaaaa ataacaactt ttttatctgc ttttgtcatt aacacggcac acaggcgtaa
    14701 tatctgacaa aactgcattt cgatttcttt cagtgcggaa atcgtcatta cccgtgagtc
    14761 tctttacatc atgaatatgc tggaaaaaat ccagtctcag ctggaacatt tgagcaaatc
    14821 agagcgcaaa gttgccgagg tcattctggc ttcgcccgat aacgcgatcc attcgagtat
    14881 tgctgctatg gcactggaag ccaatgttag cgaaccgacg gtgaatcgtt tctgtcgcag
    14941 catggacacg cgcggttttc ctgattttaa acttcatctg gcacagagtc tggcgaatgg
    15001 cactccctat gttaatcgca atgtcaatga agatgacagc gttgaatcat acacagggaa
    15061 aatatttgag tccgcaatgg caacgcttga tcatgtccgt cattcactgg ataaatctgc
    15121 catcaaccgc gccgtcgact tgctcactca ggcaaaaaaa atcgcctttt tcggattagg
    15181 ctcttcagcc gccgttgccc acgatgcgat gaataagttc tttcgtttta atgttccggt
    15241 ggtgtactcc gatgatatcg tgctgcaacg catgagttgt atgaattgta gcgacggaga
    15301 cgtggtggtg ctgatttctc acactggaag aacaaaaaat ctggtcgagc tggcgcagct
    15361 ggctcgcgaa aacgacgcaa tggtgattgc cctcacctct gcgggtaccc cgctcgcccg
    15421 ggaagcaacg ctggcaatta ccctcgacgt accggaagat actgacattt atatgcccat
    15481 ggtttctcga cttgcacagc tgaccgtgat agatgtgctg gcgacaggat ttactttgcg
    15541 acgcggtgca aaattcagag ataacttgaa gcgggtcaag gaagcgctga aggaatcgcg
    15601 ttttgataag cagttactta atttaagtga cgatcgctaa aaacgactgt cactgtccta
    15661 atcttatacg acatccgaat gagattaatt tatcgccatc gcggcgttat ttcattcgga
    15721 tttcatgttc aagcaacacc tggttgtttc agtcaacgga gtattacatg tccagaaggc
    15781 ttcgcagaac aaaaatcgtt accacgttag gcccagcaac agatcgcgat aataatcttg
    15841 aaaaagttat cgcggcgggt gccaacgttg tacgtatgaa cttttctcac ggctcgcctg
    15901 aagatcacaa aatgcgcgcg gataaagttc gtgagattgc cgcaaaactg gggcgtcatg
    15961 tggctattct gggtgacctc caggggccca aaatccgtgt atccaccttt aaagaaggca
    16021 aagttttcct caatattggg gataaattcc tgcttgacgc caacctgggt aaaggtgaag
    16081 gcgacaaaga aaaagtcggt atcgactaca aaggcctgcc tgctgacgtc gtgcctggtg
    16141 acatcctgct gctggacgat ggtcgcgtcc agttaaaagt actggaagtt cagggcatga
    16201 aagtgttcac cgaagtcacc gtcggtggtc ccctctccaa caataaaggt atcaacaaac
    16261 ttggcggcgg tttgtcggct gaagcgctga ccgaaaaaga caaagcagac attaagactg
    16321 cggcgttgat tggcgtagat tacctggctg tctccttccc acgctgtggc gaagatctga
    16381 actatgcccg tcgcctggca cgcgatgcag gatgtgatgc gaaaattgtt gccaaggttg
    16441 aacgtgcgga agccgtttgc agccaggatg caatggatga catcatcctc gcctctgacg
    16501 tggtaatggt tgcacgtggc gacctcggtg tggaaattgg cgacccggaa ctggtcggca
    16561 ttcagaaagc gttgatccgt cgtgcgcgtc agctaaaccg agcggtaatc acggcgaccc
    16621 agatgatgga gtcaatgatt actaacccga tgccgacgcg tgcagaagtc atggacgtag
    16681 caaacgccgt tctggatggt actgacgctg tgatgctgtc tgcagaaact gccgcagggc
    16741 agtatccgtc agaaactgtt gcagccatgg cgcgcgtttg cctgggtgcg gaaaaaatcc
    16801 cgagcatcaa cgtttctaaa caccgtctgg acgttcagtt cgacaatgtg gaagaagcta
    16861 ttgccatgtc agcaatgtac gcagctaacc acctgaaagg cgttacggcg attattacca
    16921 tgaccgaatc gggtcgtacc gcgctgatga cctcccgtat cagctctggt ctaccaattt
    16981 tcgccatgtc gcgccatgaa cgtacgctga acctgactgc tctctatcgt ggcgttacgc
    17041 cggtgcactt tgatagcgct aatgacggcg tagcagctgc cagcgaagcg gttaatctgc
    17101 tgcgcgataa aggttacttg atgtctggtg acctggtgat tgtcacccag ggcgacgtga
    17161 tgagtaccgt gggttctact aataccacgc gtattttaac ggtagagtaa tctcacctgc
    17221 cccggccgct gatggccggg gtatttagca acacataaat taggactact tattcaatcc
    17281 aatcataatc acagccccct gtaaaatgaa tacagggata gcctgaatgg agatctaaat
    17341 agcttgcaag acgtcgcata cccgcttcct ctgcaacact atgtccaata actgccatcg
    17401 gtaagccact atctttggag tattgtgtct gcacccatgt tttaattgaa tcgttaatcg
    17461 taatataata atcagcctga tattccatat attgtattgg atcacaaaaa cagcctgccc
    17521 caatgccaac agttctgact actctggctt tatctccata aaattgaatg ccaggaagat
    17581 tatatatttt caaatttttg gcaaaacgtt tggtaatgtt aatggcactg tcagggtcta
    17641 tagcatatac acagtacatc tcatcagaag caacaatatt tgtaacatcc agttctaact
    17701 gctgagccaa agccttagac attccgaacg taggctctcg atccatgaca tcatggcaac
    17761 gaatgattac caaattattt ttatgcaaaa actccttttt ttgctcaatt atttttaaat
    17821 aactgagttc accgcgagta taatccatta tttttctggc ccatgaaaac tcaagatcgt
    17881 caccctccag atcagcataa gaataaaatg taggctcatg tgtaataatg atattgacac
    17941 cttcaaaaac agcctttctt aaaacatcca ttgaggcaat ccagcaggtc ccaattttat
    18001 taataagtgt cgcctcagca ccaaaaataa ttttatcaac agttggttgc gaaacagcaa
    18061 ccattgaatt taaaaaacaa ttaagatccg cggctgtttt aatatgatgc attcaacatc
    18121 cttcacaggt ttcatcagca ataaaataat ttcaacattt ctgggaaacg ttttgcccat
    18181 gcagactcag agtgggtatc acctccccat a
//
LOCUS       DAESHI010000084        17956 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.085, whole genome shotgun sequence.
ACCESSION   DAESHI010000084 DAESHI010000000
VERSION     DAESHI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17956
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.085"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(227..1201)
                     /gene="cysB"
                     /locus_tag="KW011_002952"
     CDS             complement(227..1201)
                     /gene="cysB"
                     /locus_tag="KW011_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HBH7661238.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(1411..4008)
                     /gene="topA"
                     /locus_tag="KW011_002953"
     CDS             complement(1411..4008)
                     /gene="topA"
                     /locus_tag="KW011_002953"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBH7661239.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            4388..4639
                     /locus_tag="KW011_002954"
     CDS             4388..4639
                     /locus_tag="KW011_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HBH7661240.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(4675..5724)
                     /gene="sohB"
                     /locus_tag="KW011_002955"
     CDS             complement(4675..5724)
                     /gene="sohB"
                     /locus_tag="KW011_002955"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HBH7661241.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            5944..6702
                     /locus_tag="KW011_002956"
     CDS             5944..6702
                     /locus_tag="KW011_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HBH7661242.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            6699..7289
                     /gene="cobO"
                     /locus_tag="KW011_002957"
     CDS             6699..7289
                     /gene="cobO"
                     /locus_tag="KW011_002957"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HBH7661243.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(7329..8201)
                     /gene="rluB"
                     /locus_tag="KW011_002958"
     CDS             complement(7329..8201)
                     /gene="rluB"
                     /locus_tag="KW011_002958"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HBH7661244.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(8414..9528)
                     /locus_tag="KW011_002959"
                     /pseudo
     CDS             complement(8414..9528)
                     /locus_tag="KW011_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(9556..10176)
                     /locus_tag="KW011_002960"
     CDS             complement(9556..10176)
                     /locus_tag="KW011_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBH7661245.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(10173..11054)
                     /gene="yciV"
                     /locus_tag="KW011_002961"
     CDS             complement(10173..11054)
                     /gene="yciV"
                     /locus_tag="KW011_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HBH7661246.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            11192..11236
                     /gene="trpL"
                     /locus_tag="KW011_002962"
     CDS             11192..11236
                     /gene="trpL"
                     /locus_tag="KW011_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HBH7661247.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    11213..11306
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            11328..12890
                     /gene="trpE"
                     /locus_tag="KW011_002963"
     CDS             11328..12890
                     /gene="trpE"
                     /locus_tag="KW011_002963"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HBH7661248.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12890..14485
                     /gene="trpD"
                     /locus_tag="KW011_002964"
     CDS             12890..14485
                     /gene="trpD"
                     /locus_tag="KW011_002964"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HBH7661249.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            14486..15847
                     /gene="trpCF"
                     /locus_tag="KW011_002965"
     CDS             14486..15847
                     /gene="trpCF"
                     /locus_tag="KW011_002965"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HBH7661250.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15859..17052
                     /gene="trpB"
                     /locus_tag="KW011_002966"
     CDS             15859..17052
                     /gene="trpB"
                     /locus_tag="KW011_002966"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HBH7661251.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            17052..17858
                     /gene="trpA"
                     /locus_tag="KW011_002967"
     CDS             17052..17858
                     /gene="trpA"
                     /locus_tag="KW011_002967"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HBH7661252.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKVATRS"
BASE COUNT         4317 a         4749 c         4488 g         4402 t
ORIGIN      
        1 cagcgaattt acgaatcgtc tccagtgaat aattcaatct tttgacctga aatagatatt
       61 tgaaaactgg agtttgcgag gcgggtaatt agacactact ttacaggtaa tttaccctat
      121 aaatatgagg gataaaaata ttgtgcacgg tatagaaata agtaacaaaa gagaatatat
      181 tccggcacct tcgctacata aaaggtgccg aaaataacgc aagaaattat ttttccggca
      241 gttttatatc tttaaacatg acctcaattt cttcattaga gcgcaatgcg acagccgcat
      301 caacgacatc acgcgttaaa tgcggtgcaa aacgctgaat gaaatcatac atataactgc
      361 gcaagaaagt actacggcga aaaccaattt tggttgtact gtggctgaag atatcgtgag
      421 catcaacacg cacaaggtcg ggatcggcga ccggatccac cgccatgctg gcaatgaccc
      481 ctacccccag ccctaaccgg acgtaagttt taatgacgtc agcatccgtt gccgtgaaga
      541 cgatacgcgg cgttaaccct gcgcggttaa aggcagtatc cagttctgaa cgtccggtaa
      601 agccgaaggt atatgtcacc aacggatatt gcgccagttc ttcaatggta atggcttttt
      661 tgcctgccag cgggtgatcc ggagtgacta caatagcccg attccagtgg tagcacggta
      721 acatcactaa atcttcatac agatgcagcg cttctgtggc gatagcgaaa tcagcattgc
      781 ctttagagac ggcatcagca atttgtgtcg gcgagccctg gtgcatatgc aaagaaacgc
      841 ggggataacg ctcaataaag cctttgataa cgtttggtaa cgcgtagcgt gcctgggtat
      901 gcgtggtggc gatatacagt gagcctttat ccggccaggt gtgctctccg gcaaccgatt
      961 ttatggcatc gactttcgac aggacttcgc gagcgatacg aattatttct tgccctgctg
     1021 gcgttacctg cgtcaggtgc ttgccgcttc gggaaaaaat ttgaatgcct agctcgtctt
     1081 ccagcattct gacttgttta ctgatcccgg gttgtgatgt gtaaagtcct tccgctgttg
     1141 atgagacatt caggttatga ttgaccacct caacaatata gcgaagttgt tgtaatttca
     1201 tgttaaacca tccacttaga cgccatcaga acatcgtttt ttcgtcacac ctggcgggtg
     1261 actaatacga tttaataata aaaaaggtgc taactataac cactatatca tttatagcct
     1321 tagtgtatag gtgtgaagaa aaaataatat gcaataaaaa agggccgctt tcgcgaccct
     1381 ttgtttataa aaacctgaca gaattaaagg ttattttttt ccttcaaccc atttgccatc
     1441 aacataaaat gctgaccagc cagtcgcctt tccgtctttt tccgaagaga catattgctg
     1501 tttggtttta cggctaaagc gaaccatggt cttattacct tccggatcct gctgtggcgc
     1561 atcggccaga taacgcagtt tttccggcag acggtcgcgg aagcgataaa gctcttccac
     1621 cagtggcgca cgcgtttcac gcgatttcgg gaaagtgttg gcagccagga aaacacccgc
     1681 agcgccgtca cgcagcacga aataagcatc tgatttttcg cacggcagct caggtaatgg
     1741 caccggatct tctttcggtg gtgccacttc gccgttacgt aaaatcttac gtgtgttttt
     1801 acactcttcg ttggtgcagg ccatgtattt accgaaacgc cccattttca ggtgcatttc
     1861 agagccacat ttttcacact caacgatcgg gccgtcataa cctttaatgc ggaattcgcc
     1921 ctcttcgatc tcgtaaccgt cgcaggttgg gttattacca cagacatgca acttacgttt
     1981 cggatcgatg agatagctgt ccatcgccgt gccgcatttc gggcaacgac gttttgcgcg
     2041 cagcgcgttg gtttcagcgt cttcgccttc cagtacgttc agcacttcgt tttccggcac
     2101 caggttaatg gtggttttgc aacgctcttt cggcggcagc gcataaccag aacagccaag
     2161 gaataccccg gtgctcgctg tgcgaatccc cattttgcga ccacaagtcg ggcagtcaat
     2221 gctggtcaga accatctggt tcgggcgcat accaccctct tccggatctt tttcagcttt
     2281 atctaactgc tgggtgaaat ccgagaagaa gtgatccagt acagctttcc actctgcttc
     2341 gtgatttgcc accaggtcga ggctgttttc catctgtgcg gtgaagtcgt agttcattaa
     2401 ctcgcggaaa ttttcttcaa ggcgatcggt gacgatttcg ccaatttttt ccgcatagaa
     2461 acgacgattt tctactcgca cgtagccacg atcctgaatg gtcgaaatga tcgacgcata
     2521 ggtagacgga cgaccgatac cgcgtttttc cagctcttta accagcgatg cttcactgaa
     2581 acgggctggc ggcttggtaa agtgctgggc tggtgtaagt tcaacgagcg tcagagcatc
     2641 gcctttatta actgctggta agatgcgatc ttcatcgcct ttacgcaacg caggcatcac
     2701 ttttgtccag ccatcaaaac gcaaaatacg accgcgtgct ttcaggcgga aatcgcccgc
     2761 accaatggtc agcgtcgtgg agtcatattt cgctggggtc atctggcagg ctacgaactg
     2821 acgccagatt aactggtaca gtttctgcgc atctgcttcc atatccttca gcgattccgc
     2881 catcacattg acgtcagaag gacgaatcgc ttcgtgcgct tcctgtgagt tttctttgct
     2941 ggcgtactga ttcggacttt ccggcagata tttcttacca aaattatcgc tgatataacc
     3001 acgaaccata tttaccgcgt cctgactcag gttagtggag tcggtacgca tgtaagtgat
     3061 atagcctgct tcatacaaac gctgcgccat catcatggtt tttttcacgc caaatccaag
     3121 acgggtgctg gcagcttgtt gcagcgtaga ggtaataaaa ggagcgccag gtttactggt
     3181 tgtcggtttg tcttcacgtt ccagcacgct gtagcgcgct ttttccagca gacttaccgc
     3241 agcctgagtt tgttctttgt tgaccggacg gaacggtttg tcgttctgat gagtcacctg
     3301 taacgccaac gcttcaccag atggcgtggt cgtgctggca tcgacttccc agaactcttc
     3361 cggcacgaac gctttaattt cacgctcacg ctcgaccacc aggcgaaccg ccaccgactg
     3421 cacacgaccg gcagacagac cacgagcgat ctttttccat agcagcggcg aaaccatata
     3481 ccccaccacg cggtccataa agcgacgcgc ctgctgggca ttaacacgat caatattcag
     3541 ctcacccggt ttgttaaatg cctggcggat cgcgttttta gtaatttcgt taaacaccac
     3601 tcggctatag cgcgcatcat cacccccaat cacttcccgc aggtgccatg caatggcttc
     3661 cccttcgcgg tcaaggtcgg ttgcgagata gatgtggtcg gctttttcag ccagttgttt
     3721 cagttcagag acgaccttct ctttaccagg caacacttca tagtgcgcct cccaattgtg
     3781 ccacgggtca acccccatac ggttgacgag agcgccacgt tcatcctttt taggcttttt
     3841 agccgtcttg gtggaggtag agtcggcact ctttttggca gctgagccac tggtcggcaa
     3901 atcgcggatg tgaccgacgc tggatttcac cacgtagtca ctacccagat acttgttgat
     3961 cgttttggct tttgccgggg actcaacgat gacaagagct ttacccatat tcaccttacc
     4021 taatttgatt cttccaggaa tgcgtcgcac gttgataaac cttccactgg caacgagtta
     4081 ccgatatgga ctcgttctct gcggatatca accccttgtc gtgactctgt tcacgaaaat
     4141 tcaggtgact gagttaacag gtcttggcct acaggcgcta tagcacgacg gaattttggt
     4201 cgaatgtcaa gcaaatctat tgccagattg acgaaagcgt cacactgtac ctgataaaat
     4261 ccgatacgca actttattag catgcaaaat ccaaagttgc cagtaaggcg ctcttgccac
     4321 cagcgtggta tatttgtctg caagccatgc ctggcgtaga ctaatgtcct tgtttaagga
     4381 gtaaataatg aacaaagaga cacaacccat cgaccgcgaa acgctgttga aagaggccaa
     4441 caaaatcatt cgtgagcatg aggatacgct cgcagggatt gaagccaccg gcgtcacgca
     4501 gcgtaatggc gtgctggtat ttatcggcga ctacttttta gatgaacaag gattgccaac
     4561 agccaaaagc accgcggtgt ttaatatgtt caaacacctg gcccatgtgc tttcagaaaa
     4621 atatcatctg gttgattaaa gcaaaacgcg aggtcttagc ctcgcgtttg tcttttacat
     4681 caatggcttt tgaccccgct gccaccagcg tagcaacaat cgatcggcgc tctctgccgc
     4741 gctgccggtg aatcggtcaa tgagtcgttt acgctgcata tagcgtacat taaccacttc
     4801 acggccttcc atcaggctaa gaataacttc atcactggtg ttgatttcat caaccaggcc
     4861 tttctctacc gcctgttgtc cgtaccagtg ttcacccgtt gccacctgtt caatatccag
     4921 agacggacgc atacgcttca caaaatcttt aaataactga tgcgtttcgt tcagctcttc
     4981 gcggaatttc tcccgccctt cttcggtatt ttcacccagc aaggtcagcg tgcgcttata
     5041 ctgcccggcg gtgtgcagtt cgatatcaat atctttgctt ttcaggaagc ggttaaagtt
     5101 gggcatttgc gccaccaccc caatggaacc cacaatagca aacggtgcgg aaacaatttt
     5161 gtccgccaca caggccatca tgtaaccgcc gctggcagcg actttgtcta ccgtaacagt
     5221 taaaggaatg tttttatcac gcagacgctg cagctgcgaa gccgccaacc cgtaaccatg
     5281 caccatgcca ccagggcttt ccagacggag cacaacctga tcctgcggtt tgaatgctgc
     5341 gagtaccgcc gttatctctt cacgtagcga gttcacttca tgggcgtcca tgctgccttt
     5401 aaaatccagc acccagacgc gaggtttact gtcagttgcc acctcgccca gtttggcttt
     5461 cgcttttgct gctttcgctt cttgcttgtg ctttttcttc tgcgctttgt gccactgttt
     5521 ttgctgatgt gagtccatca gcgccgcggc cagttcttct ttcatctcct tatactgttc
     5581 gctgagattg ttgacccgta actcgccacg ctggcgttta ttacgttgag caacattgac
     5641 aataatggcg gcaatcgccg caatcgctag cacaacggta acgattttcg ccaaaaacaa
     5701 accatattca gacaacaatt ccacgagtcc ccaccttggt taaacaacgc tttggctcac
     5761 agtttacaac aggcatgtta agccgtctcg cagagatgtt attgcagcga aagtatgaca
     5821 aagtatccat tttgcggtgt tatcgcccca ggctgattga aatgcccttc tgtttcaggc
     5881 ataaagcccc aaagtcataa agtacactgg cagcgcgaca gacaacgtcg ttgaggagta
     5941 gccatgcatt accagccaaa acaagattta ctcaatgatc gcattatcct ggtgacggga
     6001 gccagcgatg ggattggtcg tgaagccgcg atgacgtatg cacgctatgg tgcgacagtg
     6061 attctgttgg gccgtaatga agaaaaatta cgtcaggtag ccagccacat aaacgaagaa
     6121 actgggcgtc agccacagtg gtttattctc gatttgctga cctgcacgtc cgaaaattgc
     6181 caacaactgg cacagcgcat tgtcgttaat tatccgcgtc tggatggtgt tttgcataat
     6241 gccggattgc tcggcgatgt ttgcccaatg agcgaacaaa atccgcaggt ctggcaggac
     6301 gtcatgcagg tcaacgttaa tgccaccttt atgctcaccc aggcactgct tcctttatta
     6361 ctcaaatcgg acgccggttc actggtcttt acttcatcaa gcgttggacg tcagggacga
     6421 gccaactggg gtgcatatgc agcgtcgaaa tttgccaccg aagggatgat gcaggtactg
     6481 gccgatgaat atcagcagcg cctgcgcgtc aactgcatta acccaggcgg tacgcgcacc
     6541 gcaatgcgcg caagcgcctt cccgaccgaa gatccacaga aacttaaaac acccgctgat
     6601 atcatgccgc tctacctctg gctgatgggc gatgacagcc gccgtaaaac cggcatgacc
     6661 tttgacgccc aaccgggccg taaaccagga atttcccaat gagtgatgaa cgctaccaac
     6721 agcgtcagca gcgagtgaaa gaaaaagtag atgctcgtgt ggcccaggcc caggatgaac
     6781 gcggtattat catcgtcttt accggcaatg gaaaaggcaa aaccaccgcg gcatttggta
     6841 cggcaacacg cgcagttggt cacggaaaaa aagtgggcgt cgtgcagttt attaaaggca
     6901 cctggcctaa tggcgaacgc aatctgctgg agccacatgg cgttgagttt caggtgatgg
     6961 caacgggctt tacctgggat acacaaaacc gcgagtctga taccgccgcc tgccgcgaag
     7021 tctggcaaca tgcaaagcgg atgcttgctg attcctcact ggatatggtt ttgcttgatg
     7081 aactgacgta tatggtggcg tatgactatt tgccactgga agaagtggtg caggcgttaa
     7141 atgaacgtcc acatcaacag acggtgatta tcacgggtcg aggctgtcat cgggatattc
     7201 ttgaactggc ggatacggta agtgagctgc gccccatcaa acatgcgttt gatgccggtg
     7261 ttaaggcgca aatagggatt gattattaat ctgtaaaaat gccggatatg acgtccggca
     7321 tactttgatt aaccgttatt acgcccgcca gaacgacgac cgccgctcac ctgactgtga
     7381 cgtttcaccg cacgacgaat ctgattagcc ttcatgcgac gacggtcttt ttctacagcg
     7441 actttagagc tggtttctgg cggaagctcc accagttcgc gcagatagtt agtctgggcg
     7501 aggtccagct ccgtccagcc accgcgtggc agaccttttg gcagtgggat atcaccgtaa
     7561 cgaacacgga tcaggcggct cacctgcaca ccaaccgctt cccacagacg acgaacttca
     7621 cggttacggc cttcggtcag agtcacgttg taccactggt tgatcccttc gccgccgctg
     7681 aacttgatgg ttttaaaagc tgccggacca tcttccagtt gcacgccacg actcaaatca
     7741 cgcagtttcg cgtcgtcaac ctgaccaaat acacgcacgg cgtattcacg ttcaacttca
     7801 cggcttggat gcattaaacg gtttgccagt tcaccatcgg tggtgaacag cagcagacca
     7861 caggtgttaa cgtccagacg acctacggca atccagcgtg cgccacgcag ctttggcaga
     7921 cggtcaaaca ccgtcggacg cccttccgga tcgttacggg tacacaactc accttccggt
     7981 ttgtaatagg ccagaacgcg acaaatttgt tcagcggact cacgtaccga aatcagatga
     8041 ccatcgatac gaattttcag cccaggggta acttcaacgc gatcgccgag tttagcaatt
     8101 ttgccatcaa cactcacacg accggcttca ataatagatt cgatttcacg gcgggagccg
     8161 tggccagcgc gggccagcac tttctgtaat ttttcgctca tagagcttcc ttttggtgtc
     8221 gccttcccag gcgtctgata tgcttttgtg caacagtgag tcagaactta catctgactc
     8281 actgatagta aagtctgttt gaactcttca ggccttaagt aaacgactcg acgttttaac
     8341 tggcctggct ttgaggcgca acatactaca ctatctttat ggttaaagct tgtttcgatt
     8401 aacatcagct taattaccag ccaccaccgc ctccgccacc cgcgccgcca ccagcaccac
     8461 caccggaata acttcgcccc aacaaactgg ttttggcccc cagactcagg tgcatctgta
     8521 atgaaaacaa aacgccacta cgggcaattt ccggggcgct aacagccgct gtgagtgatg
     8581 gggtgatacg tgctacccat tctcttccaa ggtttgctga gactgcccaa cctagcaacg
     8641 cgtaatccag tttccgtgtt tcccctttcc gccgacgccc actgtgtgtt gctgcctctt
     8701 tacgcgcaag atagttaacg atagcttcac cgtgggcata acgttgctgc ccagtttgtg
     8761 taaactttgg cagaaaaata taccccatgc cagtgagata accggtcagg aaaacagcgc
     8821 taaaataacc cgctggcata taccagtaaa agaatacatc tccgacattg atgtagagag
     8881 aatagaaggc cacgcctgaa caaatcagtg gtaaacaatt cttccgtcct caaggatgcg
     8941 gccatttcga tatgtgtcgc cctcttctcc agtaaagaga tcgatttcgc taaatggatt
     9001 acccgcgata tcagggaact tgagtgaaaa tcgggtctta tagatttcaa acggccagtg
     9061 gttaccagtc acgttccaga ttaacagatc tgaatctccc tcacgcagga aagcattttt
     9121 aacctgataa tgaatttgat aatgatagtc gccttgaggc aagaaacgtt gtttatcgcc
     9181 cacgacaata ctcattaacc cgatatgcca gtcatgatgc cagggttcag gaataccatc
     9241 acgggtgacg ccaacaatat gatagttcag cagtgcagcg tccccatcag gctgcatcca
     9301 gagtcgtggt aaatcacgat aaaatccgtg tttaatctgc tgatttcgcg cctgaaaatg
     9361 aatattttct ttgatgtcga gcaccccatc cggttgcaga gtggcttcaa catcaaagag
     9421 cgaaatccct tcttcataag aagggatctc cgtgttttct gcataactta gagatgatac
     9481 aaatagccct gctatcaaca gcaaaatgca gcgaaacttc cctgccatag tcttccatcc
     9541 gtggaacgca atttgttata agaaaggctt cacatcacct acgccttcac gcaccacgac
     9601 cggcgtatca tcggtaagat caataaccgt tgtcggtttc tgcccgagat agccgccgtg
     9661 aataatcaaa tctacctgtt tttccagacg gtctttaatt tcttcgggat cggattcggt
     9721 aaattcgctg cctggcagca ttagcgaagt ggaaagcatc ggttcgccca gtgcttcaag
     9781 caacgcctgc gcaatcgggt tagaaggcac acgcataccg atggttttgc gtttttcctg
     9841 caacagacgg cgtggcactt ccttcgtccc cttcaggatg aaggtatagt tgcccggcgt
     9901 gttgtttttc attaaacgaa acgcaacgtt atcaacaaat gaataggtcg agagttcaga
     9961 aagatcgcga cacatcaggg taaagttgtg accgtccggc agctggcgaa tacgacaaat
    10021 acgctccatc gcgtttttat cttcaatttt acagccgagc gcatagccgg aatcagttgg
    10081 ataaacaatc accccgcctt tacgcacgat ctccaccgcc tggttgatca gacgttgctg
    10141 tgggttatca ggatgaatat aaaaaaactg gctcataatt ccctctctgt ggtgttctgc
    10201 ggctgttccc atagctgcca gacgccttca acgcctgcgg gcagccagag tttacgacct
    10261 agttcgatcc acgggcatgg ctgatgaaaa tcagatcctt gtgatgccca taaatgatgc
    10321 tgacgcgcaa gggccgccag ctgggtacgt tcattgggcg attgctggca ctgcgcgact
    10381 tccatcgcgt caccgtggtg ttcggcaaaa tgcgcaacca atcttttcag ccatttagca
    10441 ctaagattgt accgcccagg atgagctagc accgccttac cgccagaatg atgaatgaca
    10501 tcaatagctt gttctattgt acaccactgt ggcggaacgt atccggtttt cccgcgcgcc
    10561 agatattttt taaagacatc cgccattgaa ctggctttgc cgcaccccac aaggaaacgc
    10621 gcaaaatgac cgcgcgtcac cgcaccgccc tgcgccagtc gtcgcgcgcc ttccagtgcc
    10681 cctggtattt gcgctttttc aagtctttcg gcaatcagtt gcgcccgctg attgcgccgt
    10741 tctgtctgct gcgcgaggaa ctcacacatt agcggatgag taatatcaat attcagcccg
    10801 acaatatgaa tttcatgatt ttcccagacc gtggaaattt ccacgccggg aataagattc
    10861 agcgccagtc ccgaacgtga aatttcctct cttgctggca cgattgcagc tgtgttgtca
    10921 tggtcggtga tcgccagggt accgacgcgc atctcgactg cacggtgcac caatacttct
    10981 ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcgtaa
    11041 ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac
    11101 agttctggca aatattctga aatcagctgt tgacaattaa tcatcgaact agttaactag
    11161 tacgcaagtt cacgtaaaaa gggtatcgac aatgaaagca attttcgtac tgaaagcttg
    11221 gtggcgcact tcctgaaacg ggcagtgtat tcaccatgcg taaagcaatc agatacccag
    11281 cccgcctaat gagcgggctt ttttttgaac aaaattagag aataacaatg caaacacaaa
    11341 aaccgactct cgaactgcta acctgcgaag gcgcttatcg cgacaacccg actgcgcttt
    11401 ttcaccagtt gtgtgggaat cgtccggcaa cgctgctgct ggaatccgca gatatcgaca
    11461 gcaaagatga tttaaaaagc ctgctactgg tagacagtgc gctgcgcatt acagcattag
    11521 gtgacactgt cacaattcag gcactttccg gcaacggcga agccctgctg gcactactgg
    11581 ataacgccct gcctgcgggt gtggaaaatg aacaatcacc aaactgccgt gtgctgcgct
    11641 tcccccctgt cagtccactg ctggatgaag acgcccgctt atgctccctt tcggtttttg
    11701 acgctttccg tttattgcag aatctgttga atgtaccgaa ggaagaacga gaagcaatgt
    11761 tcttcggcgg cctgttctct tatgaccttg tggcgggatt tgaagattta ccgcaactgt
    11821 cagcggaaaa taactgccct gatttctgtt tttatctcgc tgaaacgctg atggtgattg
    11881 accatcagaa aaaaaacacc cgcattcagg ccagcctgtt tgctccgaat gaagaagaaa
    11941 aacaacgtct cactgctcgc ctgaacgatc ttcgccagca gctgaccgaa gccgcgccgc
    12001 cgctgccggt ggtttccgtg ccgcatatgc gttgtgaatg taatcagagc gatgaagagt
    12061 tcggtggcgt agtgcgtttg ttgcaaaaag cgattcgcgc tggagaaatt ttccaggtgg
    12121 tgccatctcg ccgtttctct ctgccctgcc cgtcaccgct ggcggcctat tacgtgctga
    12181 aaaagagtaa tcccagcccg tacatgtttt ttatgcagga taatgatttc accctgtttg
    12241 gcgcgtcgcc ggaaagttcg ctcaagtatg acgccaccag ccgccagatt gagatctacc
    12301 cgattgccgg aacacgtcca cgcggtcgtc gtgccgatgg ctcgctggac agagacctcg
    12361 acagccgcat cgaactggaa atgcgtaccg atcataaaga gctttctgaa catctgatgc
    12421 tggtggatct cgcccgtaat gatctggcac gcatttgcac ccccggcagc agctacgtcg
    12481 ccgatcttac caaagttgac cgttactctt acgtgatgca cctggtctcc cgcgtggtcg
    12541 gtgagctgcg ccacgatctc gacgccctgc acgcttaccg cgcctgtatg aatatgggga
    12601 cgttaagcgg tgcgccgaaa gtacgcgcca tgcagttaat tgccgaggcg gaaggtcgtc
    12661 gccgcggcag ctacggcggc gcggtaggtt atttcaccgc gcatggcgat ctcgacacct
    12721 gcattgtgat ccgctcggcg ctggtggaaa acggtatcgc caccgtgcaa gccggtgctg
    12781 gcgtagtcct tgattctatt ccgcagtcgg aagccgacga aacccgtaat aaagcccgcg
    12841 ctgtactgcg cgctattgcc accgcgcatc atgcacagga gactttctga tggctgacat
    12901 tctgctgctc gataatatcg actcttttac gtacaacctg gcagatcagt tgcgcagcaa
    12961 tgggcataat gtggtgattt accgcaacca tattccggcg cagaccttaa ttgaacgcct
    13021 ggcgacgatg agcaatccga tactgatgct ttctcctggc cccggtgtgc cgagcgaagc
    13081 cggttgtatg ccggaactcc tcacccgctt gcgtggcaag ctgcccatta ttggcatttg
    13141 cctcggacat caggcaattg tcgaagctta cgggggctat gtcggtcagg cgggcgaaat
    13201 tcttcacggt aaagcgtcga gcattgaaca tgacggtcag gcgatgtttg ccggattaac
    13261 aaacccgctg ccggtggcgc gttatcactc gctggttggc agtaacattc cggccggttt
    13321 aaccatcaac gcccatttta atggcatggt gatggcagta cgtcacgatg cggatcgcgt
    13381 ttgtggattc cagttccatc cggaatccat tctcaccacc cagggcgctc gcctgctgga
    13441 acaaacgctg gcctgggcgc agcagaaact agagccagcc aacacgctgc aaccgattct
    13501 ggaaaaactg tatcaggcgc agacgcttag ccaacaagaa agccaccagc tgttttcagc
    13561 ggtggtgcgt ggcgagctga agccggaaca actggcggcg gcgctggtga gcatgaaaat
    13621 tcgcggtgag cacccgaacg agatcgccgg ggcagcaacc gcgctactgg aaaacgcagc
    13681 gccgttcccg cgcccagatt atctgtttgc tgatatcgtc ggtactggcg gtgacggcag
    13741 caacagtatc aatatttcta ccgccagtgc gtttgtcgcc gcggcctgtg ggctgaaagt
    13801 ggcgaaacac ggcaaccgta gcgtctccag taaatctggt tcgtccgatc tgctggcggc
    13861 gttcggtatt aatcttgata tgaacgccga taaatcgcgc caggcgctgg atgagttagg
    13921 tgtttgtttc ctctttgcgc cgaagtatca caccggattc cgccacgcga tgccggttcg
    13981 ccagcaactg aaaacccgca ccctgttcaa tgtgctgggg ccattgatta acccggcgca
    14041 tccgccgctg gcgttaattg gtgtttatag tccggaactg gtgctgccga ttgccgaaac
    14101 cttgcgcgtg ctggggtatc aacgcgcggc ggtggtgcac agcggcggga tggatgaagt
    14161 ttcattacac gcgccgacaa tcgttgccga gctgcatgac ggcgaaatta agagctatca
    14221 attgaccgct gaagattttg gcctgactcc ctaccaccag gagcaactgg caggcggaac
    14281 accggaagaa aaccgtgaca ttttaacacg cttgttacaa ggtaaaggcg acgccgccca
    14341 tgaagcagcc gtcgctgcga acgtcgccat gttaatgcgc ctgcatggcc atgaagatct
    14401 gcaagccaat gcgcaaaccg ttcttgaggt actgcgcagt ggttccgctt acgacagagt
    14461 taccgcgctg gcggcacgag ggtaaatgat gcaaaccgtt ttagcgaaaa tcgtcgcaga
    14521 caaggcgatt tgggtagaaa cccgcaaaca gcagcaaccg ctggccagtt ttcagaatga
    14581 ggttcagccg agcacgcgac atttttatga tgcgctacag ggtgcacgca cggcgtttat
    14641 tctggagtgc aagaaagcgt cgccgtcaaa aggcgtgatc cgtgatgatt tcgatccggc
    14701 acgcattgcc tccatttata aacattacgc ttcggcaatt tcggtgctga ctgatgagaa
    14761 atattttcag gggagctttg atttcctccc catcgtcagc caaatcgccc cgcagccgat
    14821 tttatgtaaa gacttcatta tcgaccctta ccagatctat ctggcgcgct attaccaggc
    14881 cgatgcctgc ttattaatgc tttcagtact ggatgacgac caatatcgcc agcttgcagc
    14941 cgtcgctcac agtctggaga tgggggtgct gaccgaagtc agtaatgaag aggaactgga
    15001 gcgcgccatt gcattggggg caaaggtcgt tggcatcaac aaccgcgatc tgcgtgattt
    15061 gtcgattgat ctcaaccgta cccgcgagct tgcgccgaaa ctggggcaca acgtgacggt
    15121 aatcagcgaa tccggcatca atacttacgc tcaggtgcgc gagttaagcc acttcgctaa
    15181 cggttttctg attggttcgg cgttgatggc ccatgacgat ttgcacgccg ccgtgcgccg
    15241 ggtgttgctg ggtgagaata aagtatgtgg tctgacgcgt gggcaagatg ctaaagcagc
    15301 ttatgacgcg ggcgcgattt acggtgggtt gattttggtt gcgacatcac cgcgttgcgt
    15361 caacgttgaa caggcgcagg aagtgatggc tgcggcaccg ttgcagtatg ttggcgtgtt
    15421 ccgcaatcac gatattgccg atgtggtgga caaagctaag gtgttatcgc tggcggcagt
    15481 gcaactgcat ggtaatgaag atcagctgta tatcgatacg ctgcgtgaag ctctgccagc
    15541 acacgttgcc atctggaaag cattaagcgt cggtgaaacc ctgcccgccc gcgagcttca
    15601 gcacgttgat aaatatgttt tagacaacgg ccagggtgga agcgggcaac gtttcgactg
    15661 gtcactatta aatggtcaat cgcttggcaa cgttctgctg gcggggggct taggcgcaga
    15721 taactgcgtg gaagcggcac aaaccggctg cgccggactt gattttaatt ctgctgtaga
    15781 gtcgcaaccg ggcatcaaag acgcacgtct tttggcctcg gttttccaga cgctgcgcgc
    15841 atattaagga aaggaacaat gacaacatta cttaacccct attttggtga gtttggcggc
    15901 atgtacgtgc cacaaatcct gatgcctgct ctgcgccagc tggaagaagc ttttgtcagc
    15961 gcgcaaaaag atcctgaatt tcaggcccag ttcaacgacc tactgaaaaa ctatgccggg
    16021 cgtccaaccg cactgaccaa atgccagaac attacggctg gcacgaacac cacgctgtat
    16081 ctgaagcgcg aagatttgct gcacggcggc gcgcataaaa ctaaccaggt gctcggtcag
    16141 gctttactgg cgaagcggat gggtaaaacc gaaatcatcg ccgaaaccgg tgccggtcag
    16201 catggcgtgg cgtcggccct tgccagcgcc ctgctcggcc tgaaatgccg tatttatatg
    16261 ggtgccaaag acgttgaacg ccagtcgcct aacgtttttc gtatgcgctt aatgggtgcg
    16321 gaagtgatcc cggtgcatag cggttccgcg acgctgaaag atgcctgtaa cgaggcgctg
    16381 cgcgactggt ccggtagtta cgaaaccgcg cactatatgc tgggcaccgc agctggcccg
    16441 catccttatc cgaccattgt gcgtgagttt cagcggatga ttggcgaaga aaccaaagcg
    16501 cagattctgg aaagagaagg tcgcctgccg gatgccgtta tcgcctgtgt tggcggcggt
    16561 tcgaatgcca tcggcatgtt tgctgatttc atcaatgaaa ccaacgtcgg cctgattggt
    16621 gtggagccag gtggtcacgg tatcgaaact ggcgagcacg gcgcaccgct aaaacatggt
    16681 cgcgtgggta tctatttcgg tatgaaagcg ccgatgatgc aaaccgaaga cgggcagatt
    16741 gaagaatctt actccatctc cgccggattg gatttcccgt ctgtcggccc acaacacgcg
    16801 tatcttaaca gcactggacg cgctgattac gtgtctatta ccgatgatga agcccttgaa
    16861 gccttcaaga cgctgtgcct acacgaaggg atcatcccgg cgctggaatc ctcccacgcc
    16921 ctggcccatg cgctgaaaat gatgcgcgaa aacccggaaa aagagcagct actggtggtt
    16981 aacctttccg gtcgcggcga taaagacatc ttcaccgttc acgatatttt gaaagcacga
    17041 ggggaaatct gatggaacgc tacgaatctc tgtttgccca gttgaaggag cgcaaagaag
    17101 gcgcattcgt tcctttcgtc acgctcgggg atccgggcat tgagcagtca ttgaaaatta
    17161 tcgatacgct aattgaagcc ggtgctgacg cgctggagtt aggcatcccc ttctccgacc
    17221 cactggcaga tggcccgacg attcaaaacg ccactctgcg cgcctttgcg gcaggtgtga
    17281 ctccggcaca atgttttgaa atgctggcac tgattcgcca gaaacacccg accattccca
    17341 ttggcctgtt gatgtatgcc aatctggtgt ttaacaaagg cattgatgag ttttatgccc
    17401 agtgcgagaa agtcggcgtc gattcggtgc tggttgccga tgtgccaatt gaagagtccg
    17461 cgcccttccg ccaggccgcg ttgcgccata atgtcgcacc tatcttcatc tgcccgccaa
    17521 atgccgatga cgacctgctg cgccagatag cctcttacgg tcgtggttac acctatttgc
    17581 tgtcacgagc gggcgtgacc ggcgcagaaa accgcgccgc gttacccctc aatcatctgg
    17641 ttgcgaagct gaaagagtac aacgctgcac ctccattgca ggggtttggt atttccaccc
    17701 cggatcaggt aaaagcagcg attgatgcag gagctgcggg cgcgatttct ggttcagcca
    17761 ttgttaaaat catcgagcaa catattaatg agccagagaa aatgctggcg gcactgaaag
    17821 tttttgtaca accgatgaaa gtggcgacgc gcagttaatc ccccagccgc cagttccgct
    17881 ggcggcattt taactttctt taacgaagcc ggaaaaatcc taaattcatt taatatttat
    17941 ctttttaccg tttcgc
//
LOCUS       DAESHI010000085        17457 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.086, whole genome shotgun sequence.
ACCESSION   DAESHI010000085 DAESHI010000000
VERSION     DAESHI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.086"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(271..720)
                     /locus_tag="KW011_002968"
     CDS             complement(271..720)
                     /locus_tag="KW011_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HBH7661253.1"
                     /translation="MSDLKTRLKEYEGTKEYQANLKYFRNGKFYPYADSLGCSTIGYG
                     HLIQAGEDFSTGITESEADKLLSRDLAKTIMQVQTLGLNLPDDWNDFIIIMTFQLGIG
                     GVKKFRKMLAALKAHDYLEAIKQAKCSLWYRQTPNRVNSMIAVLKNK"
     gene            802..1191
                     /locus_tag="KW011_002969"
     CDS             802..1191
                     /locus_tag="KW011_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005139042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661254.1"
                     /translation="MLSGEYAGFVYIFQFEDGSNYVGAKQIYQRVKDARKLKPTSKEN
                     GWREYLSSSNVVKNKIENGEQYSKTILWCFATMREVMIVEAILILHQMLKPECLNLAM
                     MSKIRSPNAKDKKRLLGIVQELLEYLN"
     gene            1201..1780
                     /locus_tag="KW011_002970"
                     /pseudo
     CDS             1201..1780
                     /locus_tag="KW011_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1777..2088
                     /locus_tag="KW011_002971"
     CDS             1777..2088
                     /locus_tag="KW011_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661255.1"
                     /translation="MNFEQHYGDLISKIKLNGMTPDGNSMPINKCFLDKKFQKVVKKS
                     NYQIDSYMNVIYEKKKDFKVGEVLEWELQGEKMPVFLIESKKLFVKGKHFWVYAVGII
                     E"
     gene            2085..2378
                     /locus_tag="KW011_002972"
     CDS             2085..2378
                     /locus_tag="KW011_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661256.1"
                     /translation="MISILIEFLKSGLDFFIKKKTIEQEVKKTNVEGQIETNKEEIEK
                     VTFHWRNALGFVLTLIILYNWIIVPVLDAFGIIVIQVPLGQLLQVLLIMVGGS"
     gene            2439..3233
                     /locus_tag="KW011_002973"
     CDS             2439..3233
                     /locus_tag="KW011_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBH7661257.1"
                     /translation="MSDAITTAAIKKLYGLSAGQCNICRTSLFKEQVHIGQMAHVIAK
                     SPKGPRGDDLLANDNSYENLILLCANDHILVDRDPFTYTVEKLHQIKSEHEAYIQGAT
                     DNTLFSDKKRQSDVDFLNAYFHFTPFARVRSLVETLPNSFHINLLIFGDQFDSILRDF
                     PASYPLNDKLLHGHFQSFIDSYKNIDFIISGHLPISDNRNIQVFGGANHNLLCHFNYD
                     ELPSDKALIIEQDLISHKISLLNAYEGLINYLRAYYPEVEIYSPIL"
     gene            complement(3259..3714)
                     /locus_tag="KW011_002974"
     CDS             complement(3259..3714)
                     /locus_tag="KW011_002974"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661258.1"
                     /translation="MDKKGIFLIIALLIFILLMLLLFAKLNFNVNTALAILSLILTVL
                     SGTDLVKKYTTVKSTNKSPIDMWMVFGIAISAVSMYGLYIDPQLSPLEGLTICIVSLI
                     LFVSVLVSALLNYKKIKKPDVREIIKQSHSVFRENEETITITTTIKKAP"
     gene            3749..4474
                     /locus_tag="KW011_002975"
     CDS             3749..4474
                     /locus_tag="KW011_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBH7661259.1"
                     /translation="MARISKKESANHNKVMELIHSDKLLTQEDREFIFTNYRGDGIGA
                     TGAFFTPEILAWDFVLDADCTGDCIELCAGIGRLSYYQYLRNKPTHITCVELNPEYVM
                     IGKRVLPEAEWITGNALQYSPDRFYDVAYGNPPFGKISTSKAYTGRYKGSEFEYKVIE
                     HASTFASYGAWIVPQGSAGFVYSGVHCYERRESCKYQKFVKDTGYELHPGVGIDTSIY
                     RDEWNGTKVICESVLVDDDGTNL"
     gene            4967..5713
                     /gene="mipA"
                     /locus_tag="KW011_002976"
     CDS             4967..5713
                     /gene="mipA"
                     /locus_tag="KW011_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HBH7661260.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            5803..6657
                     /gene="yeaE"
                     /locus_tag="KW011_002977"
     CDS             5803..6657
                     /gene="yeaE"
                     /locus_tag="KW011_002977"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HBH7661261.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(6708..7592)
                     /locus_tag="KW011_002978"
     CDS             complement(6708..7592)
                     /locus_tag="KW011_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HBH7661262.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(7676..8671)
                     /gene="gapA"
                     /locus_tag="KW011_002979"
     CDS             complement(7676..8671)
                     /gene="gapA"
                     /locus_tag="KW011_002979"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBH7661263.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            9013..9426
                     /gene="msrB"
                     /locus_tag="KW011_002980"
     CDS             9013..9426
                     /gene="msrB"
                     /locus_tag="KW011_002980"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBH7661264.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            9468..9740
                     /locus_tag="KW011_002981"
     CDS             9468..9740
                     /locus_tag="KW011_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HBH7661265.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(9901..10542)
                     /gene="pncA"
                     /locus_tag="KW011_002982"
     CDS             complement(9901..10542)
                     /gene="pncA"
                     /locus_tag="KW011_002982"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HBH7661266.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(10553..11569)
                     /gene="ansA"
                     /locus_tag="KW011_002983"
     CDS             complement(10553..11569)
                     /gene="ansA"
                     /locus_tag="KW011_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBH7661267.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(11736..13592)
                     /gene="sppA"
                     /locus_tag="KW011_002984"
     CDS             complement(11736..13592)
                     /gene="sppA"
                     /locus_tag="KW011_002984"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBH7661268.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            13753..14304
                     /locus_tag="KW011_002985"
     CDS             13753..14304
                     /locus_tag="KW011_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HBH7661269.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            14421..15464
                     /gene="selD"
                     /locus_tag="KW011_002986"
     CDS             14421..15464
                     /gene="selD"
                     /locus_tag="KW011_002986"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBH7661270.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            15469..17430
                     /gene="topB"
                     /locus_tag="KW011_002987"
     CDS             15469..17430
                     /gene="topB"
                     /locus_tag="KW011_002987"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBH7661271.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         4751 a         3908 c         4310 g         4488 t
ORIGIN      
        1 cgtattcctt tatactatct ttagagataa tacaaaggat acgaatgatc tattaactcg
       61 cgttgctact cttgaaacta ccattgctgt actggaaagt agtatttccc gtattgagga
      121 tgatcaggat aagatgagag atacactaag taaactggaa acacaaatcc atgaacttga
      181 tgttaagatt gaaaggatta ttgcaattct tcagcagaaa caacgaccat aaaaaaaggc
      241 atagtatgtc attcgtacta tgccttttct ttatttgttt ttaagtactg caatcatgga
      301 attcacacgg tttggtgtct gcctgtacca tagagaacac ttagcctgtt taattgcttc
      361 aaggtaatca tgtgctttca gggctgctaa catcttacgg aatttcttca ccccgccaat
      421 acctaactgg aaggtcatga taataataaa atcattccag tcatcgggaa gatttaaccc
      481 taatgtctgt acctgcataa ttgttttggc taaatcgcga gatagtaact tgtctgcttc
      541 gctttcagta atgccagtac taaaatcttc tcccgcctga attaagtgac catatccaat
      601 agtactacat cctaaactat cagcatatgg atagaatttc ccatttctga agtacttcaa
      661 atttgcctga tactctttag taccttcata ttcttttaat cgtgttttta aatcgctcat
      721 ttactaaata cctataatga atttatagga atatttatga tgagtgaatg gaaatactcg
      781 gatgattgga gcgaggagga aatgttatcc ggtgaatatg ctggtttcgt ttatatcttt
      841 cagtttgagg atggttctaa ttatgtcggt gctaagcaaa tctatcagcg ggttaaggat
      901 gcgagaaagc taaagcccac ttcaaaggaa aatggctggc gtgagtacct cagtagttcc
      961 aatgtcgtta aaaacaaaat tgaaaatggt gaacagtact caaaaacgat tctatggtgc
     1021 ttcgcaacta tgcgagaggt aatgattgta gaggcaatac tgatccttca tcagatgctt
     1081 aaaccggaat gtttgaatct ggcaatgatg agtaagatta gatcaccaaa tgcaaaggat
     1141 aaaaaaagac tactcggaat agtacaggaa ctattggagt acttgaattg aggtaaatat
     1201 atggcaagtg gaataaatgg gattggtagt ataaggaact atatacaaac gcagggagtt
     1261 agattaaata atcaattcca gaatgaaata attaaacgtt ccaagctact atcccaaaga
     1321 atgcagggtg atttgaataa tagcgttgac cgtggggcag tgggctttac tcaacgttcc
     1381 atccttttct tctatagaaa ggttgggaga aattcagtta catcgaccat catggttaaa
     1441 gatattcagg ctaagtacct ttatcaagta ctggtacagc caaaggcaat tgataagttt
     1501 atacctactt catctacaag attaactaag cagggcaaca tttcaggact gaaaaagaat
     1561 cttttaagtg aacgatataa ggtagtgaag ggtaagaatg gtaaagaacg attaattgat
     1621 accgcaaaga aagataccaa gaagaaaacc aaacgtgtaa ttggcttacg tgagaagaaa
     1681 caacgtcgcc ttatctattg acttctataa ggaagccaat gatggtgtaa gggtaattat
     1741 gagcggtatt cagggaacat tcaggataag tagacaatga attttgagca acattatggt
     1801 gatttaataa gcaagattaa gctaaatggc atgacaccag atggtaatag tatgccaatt
     1861 aacaaatgtt ttcttgataa gaaatttcaa aaggtcgtta agaaaagtaa ttatcagatt
     1921 gatagctata tgaacgttat ttatgagaag aagaaagatt tcaaagttgg tgaagtactt
     1981 gaatgggaat tacagggtga aaagatgcct gtatttctaa ttgagagtaa aaagctcttt
     2041 gtgaagggta aacatttttg ggtatatgcc gtggggatta ttgaatgatt agtatattga
     2101 ttgagtttct gaagtcagga ttggattttt tcattaagaa gaaaactatt gaacaggaag
     2161 taaagaaaac gaatgtagaa gggcagattg aaacgaataa agaagagatt gaaaaggtaa
     2221 cattccactg gcgtaatgcc ctcggttttg tactaaccct tatcattctc tataactgga
     2281 tcatagtacc agtacttgat gcttttggaa ttattgtgat ccaagttcca ttaggacaac
     2341 ttttgcaagt gctattgatt atggttggtg gaagctgaaa gatttttcct ttaatatgtg
     2401 ttttgagtta ataaaatagc aatctaagga tttgatttat gagtgatgca attactacag
     2461 cagcaataaa aaaattgtat ggactatccg ctgggcaatg taatatttgc cgaacttctc
     2521 tttttaaaga gcaggttcac atcggacaaa tggctcatgt aatagccaaa agcccaaaag
     2581 gaccaagggg cgatgacctg ttagcaaatg acaattctta tgaaaatctt atcctgcttt
     2641 gtgcaaatga tcatattctt gttgatagag atccattcac ttatacagta gaaaaattgc
     2701 atcaaatcaa aagtgaacat gaggcttata tacagggtgc aaccgataat acattatttt
     2761 cagataaaaa aagacagtca gatgtggact ttttaaacgc ttacttccac tttacaccgt
     2821 ttgcgagggt acgttcatta gttgaaactt tacctaattc ttttcacatc aatcttctta
     2881 tttttggaga tcagtttgat agtattttaa gggattttcc agcgagctat cctttaaatg
     2941 ataaattact ccacggtcat ttccaaagtt ttattgattc gtataaaaat attgatttta
     3001 tcatatctgg acatttgccg atttccgata atagaaacat tcaggtgttt ggtggtgcga
     3061 atcataatct tctttgtcat ttcaattatg atgagctacc atccgataaa gcactgataa
     3121 tagaacaaga tttgatttca cataaaatat ctcttcttaa tgcttatgaa ggtcttataa
     3181 attaccttag agcttattac cctgaggtgg aaatttactc tcctattcta tgagtgttat
     3241 gaaatatata aaagcccctt atggggcttt ttttatggtt gttgtaatag taatggtttc
     3301 ttcgttttct ctaaaaaccg aatggctttg ttttattatt tccctaacat ctggtttctt
     3361 tatttttttg taatttaata aagccgaaac taaaacagaa acaaaaagta taagtgatac
     3421 tatacaaata gttaaacctt ctaaaggtga tagttggggg tctatatata atccatacat
     3481 agaaacagcg gagatagcaa taccgaaaac catccacata tctatagggc ttttatttgt
     3541 cgatttgacc gtagtgtact ttttgactaa gtccgtacct gatagcactg ttaaaattaa
     3601 tgataatata gcgagggcag tatttacatt aaaatttagc ttagcgaata aaagcaacat
     3661 tagtaaaata aaaataagta acgctatgat taaaaatatt cctttcttgt ccataaatac
     3721 cccgtcgtat ttttaaaaaa ggtatcttat ggcaaggata agtaaaaagg aatctgctaa
     3781 tcacaataaa gtgatggaat taatacattc agataagctc ctaacacaag aagataggga
     3841 attcatattc accaattata ggggggatgg gataggggct acaggtgcct tcttcacacc
     3901 tgaaatttta gcatgggatt ttgtacttga tgctgactgt actggtgact gtattgaact
     3961 atgtgctggt atcggtcgcc tcagctacta ccagtactta aggaataaac caacgcatat
     4021 tacatgcgtg gaactcaatc ctgagtacgt aatgatcggg aagagggtac taccagaggc
     4081 agaatggatc actggtaacg cactacagta ctcaccagac cgtttctatg atgttgccta
     4141 tggtaatcca ccctttggga agatcagtac ctcaaaagcg tatacaggcc gttataaggg
     4201 tagtgaattt gaatacaagg tgattgaaca tgcaagtacc tttgcgtcct atggtgcatg
     4261 gatagtaccg caaggttccg caggctttgt ctacagtggg gtacattgtt atgagcgtag
     4321 agaatcatgt aagtaccaga agtttgtgaa ggatactggc tatgagctac atccaggtgt
     4381 aggcatcgat acaagtatct acagggatga atggaatggt actaaggtga tatgcgaatc
     4441 ggtgttagta gatgatgacg gtacaaatct gtaactaaat tagttaagaa atcataaaga
     4501 tagtagtact tgcaaatgtt cccattgatg aagttatgcg tcctttcgtt ttttagcaca
     4561 ggttaagagc cgttcatatg aatagtagag gctcccggaa gaggtaaact gaacgacgtc
     4621 tcttgctaaa acacagcaac tctcatgcca ttttatcgac gatgagaaca acgtgcccgg
     4681 tgatacacct tcaaaattaa gcgtagatgg caaatggaaa acttgcctgc acggctttct
     4741 taatttcaat ggcatatcaa caactcatcc ataaattttg cataattaat gtaaagacaa
     4801 ggctcgccag taacgctaaa ttcatttggc tgtaagcgcg gtgtcatccg cgtcaggaaa
     4861 attaaacagt tactttaaaa aatgaaaacg taaaaaggtt gggtttcgat gtattgacgg
     4921 gtaaactttg tcgcccgcta aacatttgtt taaggaatga ttaattgtga ccaaactcaa
     4981 acttctggca cttggagtgc ttatcgcaac gtctgcaggc gtagcgcacg ctgaaggtaa
     5041 attttccctg ggcgcaggcg tcggtgtcgt tgaacaccca tataaagatt acgataccga
     5101 tgtttaccca gtaccggtaa tcaactatga aggcgataac ttctggttcc gtggcttagg
     5161 tggtggttac tacctgtgga atgacgcaac ggacaaactt tcaattaccg cttattggtc
     5221 gccgctttac ttcaaagcga aagacagtgg cgatcaccaa atgcgtcacc tggatgaccg
     5281 taagagcacc atgatggctg gtctgtctta tgctcacttt acccagtacg gttacctgcg
     5341 taccaccctg gctggcgata ccctggataa cagcaacggc attgtctggg atatggcctg
     5401 gttgtatcgt tacaccaacg gcggcctgac agtgaccccg ggtatcggtg tgcagtggaa
     5461 cagcgaaaac cagaacgaat actattatgg cgtatcgcgc aaagagtccg ctcgcagcgg
     5521 tctgcgtggc tataacccga acgacagctg gagcccttac ctggagctga gcgccagcta
     5581 caacttcctc ggcgactgga gtgtttacgg taccgcgcgc tatacccgtc tgtctgatga
     5641 agttactgac agcccgatgg tggataaatc ctggactggc ctgatttcta ccgggatcac
     5701 ctacaaattc tgataatgca tctttccagg gcattttgtg cataaaaaca gggcgtcacg
     5761 ccctgttttg cattacggtg gtgcaatacc tgggaggaaa caatgcaaca aaaaatgatt
     5821 caatttagtg gcgatgtctc actgccagcc atagggcagg gaacatggta tatgggcgaa
     5881 gatgccagtc agcgcaaaac agaagttgct gcactacgcg cgggcattga gctcggttta
     5941 accctcattg ataccgccga aatgtatgcc gatggcggtg ccgaaaaggt ggttggggaa
     6001 gcattaaccg gtctgcgtga taacgtcttt ctcgtctcta aagtctatcc gtggaatgct
     6061 ggcgggcaaa aagcgataaa tgcatgcgaa gccagtttac gccgtctcaa tactgattat
     6121 ctcgatctct acttattaca caggtctggc agtttcgctt ttgaagagac tgttgcagcg
     6181 atggaaaaat tgatcgccca gggaaaaatc cgccgctggg gcgtttctaa ccttgattat
     6241 gctgatatgc aggaactctg gcagctgccg gggggaaatc agtgtgccac taatcaggtg
     6301 ctttaccatc tcggttcacg agggattgag tacgatctac tcccctggtg ccagcaacag
     6361 cagatgccgg tgatggctta cagtccatta gcccaggccg ggcggttgcg caatggactg
     6421 ttaaaaaacg cggtagtcaa cgaaattgca catgctcaca atatcagctc ggcacaagta
     6481 ttgttggcgt gggtaatcag ccatcagggt gtgatggcga ttccaaaagc ggccacgatt
     6541 gcccatgttc aacagaatgc ggctgtgctt gaggtcgaac tttcgtcagc ggaattaacc
     6601 atgctggata aagcgtatcc ggcaccaaaa ggaaaaactg cgctggatat ggtgtgatgt
     6661 tgatacgtgc cggatgcatc gcacccggca cacaacatta acgcaaatta acgtttcgca
     6721 acgcgaatgg attgcgccag atgtgcaggt ttctctttgg tgactttttg cgtttctgaa
     6781 gcgtaagccg tttctacaca aacaaatgtt ttgtagccat catccggcat atcgcccatg
     6841 ctaattgaaa gcgccggtcc cgggttccag cctacaacgt tcagatgatg ctggtggcct
     6901 acggcgataa tacgattcag cgcttcatca ttaatcacgc tgcaatcttg cggattcaga
     6961 tacacgcgat cggtacggtc agggaaggtc tgaataccgt cggtcagtac atcttctttc
     7021 gcgtcattca ctttatcaat gaagcgatcg cccagcccac tgacgcttac cttagcgata
     7081 tcacccacgt taaagtaggt atgcagggca gaggtggttt caaattcgcc atgtgattca
     7141 agatcgattt cacaggtttt acccacgcgg aaatgcgcta acagcgtaaa gtcgtgcgga
     7201 cagaattttt tcgtctcttc gctttgcgtc aattcaaaag tcagcgctac gccatcagca
     7261 tcttcattat gtgatttcag cgtccacggc aggttgcggg caaaaccgtg cgcaggcaga
     7321 ccttgttgtg ccgccggacc aaaccacggc cagcaaaccg gtacgccacc gcgaatagcg
     7381 acgccatttt tgaacggtgt gttgttgctc aaccacagaa cttcttcttc acccgcaggt
     7441 ttccacgaga gaaggtgtgc gccctgtaat gcaaaagagg cttttacttg gggatgatcg
     7501 accacaatga ggtccagttc atccagttta cgacgggaga ggacagggga gatttgttcg
     7561 atgaccggaa gggcaaaaat tttcttaatc atgacgcagt cctttaactt cattttatca
     7621 ggtaaaaaaa agagcgaccg aagtcgctct ttttagatca cagtttcatc tcaacttatt
     7681 tggagatgtg agcgatcagg tccagaactt tgttggagta accggtttcg ttgtcgtacc
     7741 aggataccag tttcacgaag ttgtcgttca gagcgatacc agctttagca tcgaacacgg
     7801 aagtgcaaac ttcgccgttg aaatcggtag atactacgtc atcttcggtg tagcccagaa
     7861 cgcctttcat ttcgccttca gcagcggctt taacggcagc tttgatctgc tcgtaagttg
     7921 cagctttttc cagacgaacg gtcaggtcaa ctacggatac gttcggggtc ggaacgcgga
     7981 acgccatacc agtcagtttg ccattcagtt ctggcagtac tttacctaca gctttagcag
     8041 caccggtaga ggacgggatg atgttctggg aagcgccgcg gccgccgcgc cagtctttgt
     8101 gagacgggcc atcaacggtt ttctgagtag cggtagtagc gtgaacggtg gtcatcagac
     8161 cttcgatgat gccgaagtta tcgttgataa ctttagccag cggagccagg cagttggtgg
     8221 tgcaggaagc gttggaaacg atgtcctggc cagcatattt gtcgaagtta gcgcctttaa
     8281 cgaacatcgg agtgttgtct ttagacggac cagtcataac cactttcttc gcaccagcgg
     8341 tgatgtgttt acgagcagtt tcgtcagtca ggaacagacc agttgcttca gcgacaacgt
     8401 caacaccaac ttcgtcccat ttcaggttag ccggatcacg ttcagcggta acacggattt
     8461 ttttaccgtt aacgatcaga tgaccgtctt tcacttcaac ggtaccgtcg aaacggccgt
     8521 gagtggagtc atatttcagc atgtatgcca tgtaatcagc gtctaacagg tcgttgattg
     8581 caacgatctc gatgtcagaa cgtttctgag cagcacggaa aacaatgcga ccgatacggc
     8641 caaaaccgtt gatacctact ttgatagtca tatattccac cagctatttg ttagtgaata
     8701 aaaggttgcc tgtaaaatta caaaaacctt acgcagcgtc aagcggaatc gtgtcaatca
     8761 ttgcgacaaa tcaatcctgt gcctaagcat tacgcgactg actcgcctca ctcttccttt
     8821 gggcttgaaa ccacatgggg tcggcgcccc gaattttaaa gggcaattac gataaaaatg
     8881 tgattttcat cacgatttag gtgcagccat ttcgtcgaga tcaagtttag gacaaaattt
     8941 tgccagttgg tgttaatgtt ttgttagaat cagtcacgta atgtgagcac gattaaagtg
     9001 agatgtgagc aaatggctaa taaaccttcg gcagaagaac tgaaaaaaaa tttgtccgag
     9061 atgcagtttt acgtgacgca gaatcatggg acagagccgc catttacggg tcgtttactg
     9121 cataacaagc gtgacggcgt atatcactgt ttgatctgcg atgccccgct gtttcattcc
     9181 caaaccaagt atgattccgg ctgtggttgg cccagtttct acgaaccggt gagtgaagaa
     9241 tccattcgtt atatcaaaga cttgtcacat ggaatgcagc gcatagaaat tcgttgcggt
     9301 aactgtgatg cccatctggg gcatgtcttc cctgacgggc cgcagccaac gggcgaacgt
     9361 tattgtgtta actctgcttc tttacgcttc actgatggcg aaaacggcga agaaatcaac
     9421 ggttgaaaaa acgattcagc aacttattcc acaggagcgg aaaaattatg aatcttgatg
     9481 acattatcaa cagcatgacg cctgaggtat accagcgttt gtcgaccgcc gttgaactgg
     9541 ggaaatggcc cgatggcgtt gcgttgaccg aggaacaaaa agcgaactgc ctgcaactgg
     9601 tgatgctgtg gcaagctcgc cataataccg aagcacagca tatgacaatt gacacaaatg
     9661 gtcagatggt catgaagagc aaacagcagt taaaagaaga ttttggtatc tcagcaaagc
     9721 ctattgctat gtttaagtga atgagttatc tagtatttaa gcctgttatt aagggagggc
     9781 aaatatactt gttaggacgt cgtgttttag ctgcaagtag gttaaatgca acactgacaa
     9841 gtattatctg gcctacaatg tcattgagga aatgtaggcc agagatgaat tcaactaata
     9901 ttacccctgt gtctcttccc agtctgccag cgtatatagc gttgccccag ctgctgacat
     9961 ctccataaac gcgtgcgcac tgtcctgggg ctggatattc acgccacgac aaccatcggt
    10021 aatcacattt accttataac ctaactgtaa cgcgtccagc acggtaaact tcacgcaata
    10081 gtcagtagcc aggcccataa cgatcaattc atcgatttca tgatcgcgta accagtcatc
    10141 gagcgaggtt ttctgccgac ggccgttatc aaaaaaggca ctgtaactgt caactaaagg
    10201 attttcgcct ttatggaaca ccgctgcgat cgctttttgg ttcagtaacg gatgtaattg
    10261 tgcgccttca ctgttctgca cacagtgatc tggccagaag gtttgtggca aaccgtcgag
    10321 ttggcctggc gtataaggct ctacaccgtg ctgactggca aaactgccgt gattcgccgg
    10381 gtgccagtcc tgactggcga taaccgcttc accgcgcgac tggcaccagt caatcaggcg
    10441 gttagcgaca tccaccgtac tgtcaccttc cggcacggcg agcgcgccac cagcacagaa
    10501 atcattttgt aaatcgacca gtaacagggc gcgagggggc attacagtct ccttaatcat
    10561 ccggcgtcag ttcgccgcgc aggttttggc tcatggcctt gcgaatggtt tcagtatcca
    10621 gttcctggct cagcaggtaa tgcagtttgg ttagcgtggc ttctacagtc atatctgcac
    10681 cgccaataac gccggcatgg gcgagggcgt taccggtggc ataaccaccc atattcactt
    10741 taccggacat acattgtgtc aggttgacca ccacaatacc gcgatcgctg gcttcttgta
    10801 attcctgcag gaaggctttg ttttgtggcg cattacccac gccataggag cgcagaatca
    10861 atgctttcac cggttggcgc agaaaattgc gcacgacgtc agcagaaatc cctggataaa
    10921 tcgtcactac gccaattggt tgtggcgtga ttggatgaac gattaattcc ccttcaccgt
    10981 gcggggcggg tggcgtattc aaacgacgaa tatggatacc agcttccagt aacggaggaa
    11041 ggtttggaga ggcaaacgca tcaaaaccat cggcatgggc tttggtagtg cggttgccgc
    11101 gatacaatcg gttattgaaa aagagcgtta cttcgttgat cggataattc gccgcaacgt
    11161 acaacgcatt cagcagatta atttgtccgt cagagcgtaa ctcagccagc gggatttgtg
    11221 accctgtcac aatgaccggt ttaccgagat tctcgagcat gaacgacagc gcagaggcag
    11281 tatacgccat cgtgtcggtg ccgtgcagga tgacaaaacc atcatagtcg tcatagtgcg
    11341 ctttaatatc ttcagcaata tgctgccagt cttctggcgt catatcagaa gaatccatca
    11401 gcggcgtata ttcatgaatg gtgaaatctg gcatctccgg gcgatggaat tccggcatca
    11461 gcgccagttg gcgttgtaga tgacctgaca ccggaatata accctgctcg gaacgctgca
    11521 tcccgatggt cccgcccgtg taggcgacgt aaattgattt cttttgcatg atattgatat
    11581 gaatgatatc gaaagagcaa aagtatatac gtgaggcagg aggagcgcac ctgatactat
    11641 gcggataagg aaatatgggg cgagacagcg ggaagaggat tcgtcgacgt aaaaaataat
    11701 gtggccgacg atctgtcggc cactcagtac aagacttaac gcacgttggc gcaggtcagg
    11761 caaaacgcat aacggttttg tgggtcgtta aacgcggcca gcttgtcgct ttcactttta
    11821 acggtagagg ctaccgaggc aagcggtgca ggtaacatgg cctggaacgc atctggcaac
    11881 attgcccgga cagaaccaga catgttgtcc atcactttgt cgaagaaggt cggttcatca
    11941 acgtagtatt ccagatgcca ctgtttcact tttgccagct ctgctgcttt ggcgaccgca
    12001 tcatcgaaat ccccgagact atcgaccagc ccgttagctt ttgcatcctg accggtccag
    12061 acgtggccct gggcgatttt atcaatctgc tccggcgtcg aatgacgcgc atcagcaacc
    12121 agcgtgataa agcgtttata gccattctca atgcttaatt gcatcatctg ctgcgcttcc
    12181 ggcggcagtg ccctggtgat agaaacatcc gccagcggtg aagttgagac accatcagta
    12241 tgaacaccaa tcgaatccag actattttct acggtggtga tcacgccgaa gataccgata
    12301 gaaccggtca gggtgctggg gttagccaca atgtaattag ctggcgtgga aatccagtaa
    12361 ccaccagatg ccgccatgcc gcccatcgat acaaccacag gcttacccgc tgcccgggct
    12421 gctgccagtt cagcgcgaat cacttcagac gcggtaacgc tgccgcctgg gctattaaca
    12481 cgcaggacaa tcgctttcac tttcgggtca aggcgagcgt cgcggatttg tgccgcagtg
    12541 gtatcaccgc caacattccc ctgagtttcc tcgccatcca taattgcgcc attagcgaag
    12601 acgacaccga tgctgtcacc ggtatctgcc ggcgttttca atgcgtaatc gtaataactg
    12661 atggcgcgat aatttttatc agtcttactc cagccgaact ctttggttag agctttttcg
    12721 atttcggcac tcgatgccag tgcatcgacc agcttgtttt ccagtgcata tttcgcggta
    12781 tcgccaccgg ttttggttaa accctcaagc aacccttgcg cgccagggaa tacctgctga
    12841 gcagggatct gccggttagc ggcaacagta ttcagatagt tttgccacag ctcaccaatc
    12901 cagcggctgt cagcttcgcg ggctgccggt gacatgtcat cacgaataaa cggttcaacg
    12961 gcagatttat acgtacccac gcggaacaca tgggtggaaa ctttcagctt atccagcaac
    13021 gatttgtagt acagaccgtt ggtagcaaaa ccgtgcaaat ccaccacgcc ttgcggtgac
    13081 agccaaattt tattggcgaa actggcgaga taatattgcc cctggctgta gttctcgcca
    13141 acggcataaa ccggtttccc gctgtcacga aactctttca gggctttgcc gatgtactgc
    13201 atagacggct ggtcgccgcc tgcgaagttt ttcagatcca tcacgatacc ggtgatattg
    13261 cggtcgtcct tcgcctggcg aatggtgttg acgatatcaa acagtgagtt ttcctgcaga
    13321 cgatcggaac tggcaccaag cagctggcgg cttaatttac taaaccgctg agaactgtcg
    13381 ggtttatcga cgatcacacc agaaatgtcc agcagcagtg cgccacgact ggccgtttct
    13441 ttcgaatcac caccactgac ctgcatccag atccccacac caaccagtac gaggaaaata
    13501 aagaacaggt taagtaccat ttcacggacg aaattcagca gacgccacgt ccatttaaaa
    13561 aatccggcaa taaatcgcca aagggttcgc atgtattctc ccaacttaag gtcacacctg
    13621 tcacaggcgc aatagatgtg gttatcgtaa tgacccaacc ggcacttgtc agcaggaatt
    13681 gcggactgtg ctgtaacaaa atccactctc gtgttaattt tgtgaataaa tatcacgaca
    13741 ggagttaatc aaatggatgc actcgaacta ttgatcaatc gccgtagcgc ctcccgcctg
    13801 gctgaacccg cgccaacggg tgaacaactg caaaacatcc tgcgtgcggg tatgcgtgcg
    13861 ccggaccata agtccatgca accgtggcat ttttttgtga ttgaagggga agggcgcgag
    13921 cgtttcagcg ccgtactgga acagggggcg attgctgccg gtagtgatga caaagctatc
    13981 gacaaagccc gtaatgcgcc gttccgcgca ccgctcatca tcacggtggt ggcgaaatgc
    14041 gaagaaaatc ataaagtccc gcgatgggaa caggaaatgt ctgccggatg cgcggtcatg
    14101 gcgatgcaaa tggcagccgt tgcccaggga tttggcggca tctggcgcag tggcgcatta
    14161 actgaaagtc cggtagtgcg tgaagcattc ggttgccgtg agcaggataa aattgtcggt
    14221 tttctctacc tcggcacgcc gcagttgaaa gcatctacgt cgattaacgt cccggacccg
    14281 acgccgtttg taacttattt ctgataattg tcgcgaaact gtctggattg tgagcaaagg
    14341 cgcaggaatt cagacactct cacttatcac ttcacggaat gagggttacc atacccggat
    14401 tgcattgaca ggagatgtcc atgagcgaga actcgattcg tttgacccaa tacagccacg
    14461 gagctggttg cggctgtaaa atttccccaa aagtgttgga aaccatcctg cacagtgagc
    14521 aggcgaagtt tgttgatccg aatttgcttg tgggtaatga gacccgcgac gatgcggcgg
    14581 tgtacgatct gggcaatggc accagcgtta tcagtaccac cgacttcttt atgccgatcg
    14641 ttgataatcc tttcgatttt ggccgcattg cggcgactaa cgccatcagc gatatcttcg
    14701 cgatgggggg caaaccgatt atggcgattg cgatcctcgg ctggccgatt aacaaacttt
    14761 ccccggaaat tgcccgcgaa gtaaccgaag gtggacgcta tgcatgccgt caggcgggta
    14821 ttgcgctggc tggcggtcac tccatcgatg cgccggagcc gatttttggt ctggcggtaa
    14881 cggggatcgt accgaccgag cgggtgaaga aaaacagtac cgcacaagcc ggatgcaaac
    14941 tgttcctgac gaaaccgctg gggatcggcg ttcttaccac ggctgagaaa aaatcactgc
    15001 tgaaaccaga acatcaggga ctagcgacgg aagtgatgtg ccggatgaac atcgcaggcg
    15061 cgtcctttgc caacatcgaa ggcgtaaaag cgatgaccga cgttacgggt tttggtctgc
    15121 tgggccattt gagcgaaatg tgtcagggcg caggtgtgca ggcgcgcgtt gactatgacg
    15181 cgatcccaaa attgccgggg gttgaagagt acattaagtt gggcgcagtg cctggcggca
    15241 ccgaacgtaa ctttgccagc tacggtcatc tgatgggtga aatgccgcgt gaagtgcgcg
    15301 atttgctgtg cgatccgcaa acttctggcg gtttgctgct ggcggtcatg ccggaagcag
    15361 aaaatgaggt caaagctaca gccgccgagt ttggcattga actgacggca attggcgaac
    15421 tggtgccagc gcgcggcggt cgtgccatgg ttgagattcg ttaattcaat gcggttgttt
    15481 attgccgaaa aaccgagtct ggcgcgcgcc attgctgatg tcctgcccaa accgcaccgg
    15541 aaaggcgatg gctttatcga gtgcggtaat ggtcaggtgg tgacctggtg tatcggtcac
    15601 ctgcttgagc aggcgcagcc agacgcctac gacagccgct atgcgcgctg gaatcttgcg
    15661 gatttgccga ttgtcccgga aaagtggcaa ttacagcccc gaccctccgt gaccaaacaa
    15721 cttaacgtca tcaaacgctt cctgcatgaa gccagcgaaa tcgttcacgc cggggacccg
    15781 gatcgtgaag ggcaactgct ggtggatgaa gtgctggact atctgcaact ggcaccggaa
    15841 aagcgccagc aggtgcagcg ttgcttgata aacgacctga acccgcaggc ggttgagcgg
    15901 gcgctcgacc gtcttcggtc caacagtgag tttgtgccat tgtgcgtttc tgcgctggcg
    15961 cgagcgcgtg ccgactggct gtacggcatc aatatgaccc gtgcgtatac cattctcggt
    16021 cgcaatgccg gttatcaggg cgtactttcc gtgggacgcg tgcagacacc cgtgctcggg
    16081 ctggtggtgc gccgcgatga agagattgaa aacttcgtgg cgaaagactt ctttgaagtc
    16141 aaagcacata tcgtgacacc tgccgatgag cggtttaccg ctatctggca accgagcgaa
    16201 gcgtgtgaac cgtaccagga tgaagaaggg cgcttgttac atcgtccact ggcggagcat
    16261 gtggttaacc gcattagtgg tcaaccagca attgtcacca gctataacga taaacgggaa
    16321 tcagaatccg cgccgctgcc tttttcgctt tcggcgttgc agattgaagc ggcaaaacgc
    16381 tttggtctga gcgcgcagaa cgtgcttgat atctgccaga agctgtacga aacgcacaag
    16441 ctaatcactt atccgcgttc cgattgtcgc tatttgccag aagaacattt tgccggacgc
    16501 cacgcggtga tgaatgccat cagcgttcat gcaccagatc tgttgctgca gccagtggta
    16561 gatccagata tacgcaaccg ctgttgggat gacaaaaagg tcgatgcaca ccacgccatc
    16621 atcccgaccg cacggagttc agccatcaac ctgacggaga acgaagcgaa ggtctataac
    16681 ctgattgccc gtcagtactt gatgcagttc tgcccggatg tggtgttccg caagtgtgtt
    16741 atcgaactgg acattgccaa aggcaaattt gtcgctaaag cgcgttttct tgctgaagca
    16801 ggctggcgca cgctgttagg cagcaaagag cgcgatgaag aaaacgacgg tacgccattg
    16861 cctgtggtgg cgaaaggcga tgagttgctg tgtgaaaaag gtgaagtggt agagcggcaa
    16921 acccagccgc cgcgccattt taccgatgca acactgcttt cggcgatgac cgggatcgcg
    16981 cgctttgtgc aggacaaaga tctgaaaaag atccttcgtg cgaccgatgg tctggggaca
    17041 gaagcaaccc gtgccgggat tattgaactg ctgttcaagc gtggttttct gaccaaaaaa
    17101 gggcgctata tccactctac cgacgccgga aaagcgttat tccattcgct gccagaaatg
    17161 gcgacgcgac cggacatgac cgcgcactgg gaatcggtgc tgacgcaaat cagcgaaaag
    17221 cagtgtcgct atcaggactt tatgcagccg ctggtgggga cgctatatca gcttattgat
    17281 caagccaaac gtacgccggt gcggcagttt cgcggcattg tggctccggg cagtggtggc
    17341 agtgctgata agaaaaaggc tgcaccgcgt aaacgtagtg cgaaaaaaag tccgccagca
    17401 gatgaagtcg gaagcggggc gatagcgtaa gcgagtgaat ctttcgtgct attcgag
//
LOCUS       DAESHI010000086        17448 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.087, whole genome shotgun sequence.
ACCESSION   DAESHI010000086 DAESHI010000000
VERSION     DAESHI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17448
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.087"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..502)
                     /locus_tag="KW011_002988"
     CDS             complement(<1..502)
                     /locus_tag="KW011_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBH7661272.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEV
                     AARLQQR"
     gene            complement(688..772)
                     /locus_tag="KW011_002989"
     tRNA            complement(688..772)
                     /locus_tag="KW011_002989"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Leu,seq:caa)
     gene            968..1987
                     /gene="ahr"
                     /locus_tag="KW011_002990"
     CDS             968..1987
                     /gene="ahr"
                     /locus_tag="KW011_002990"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HBH7661273.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="KW011_002991"
     CDS             complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="KW011_002991"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBH7661274.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            2771..3802
                     /gene="idnD"
                     /locus_tag="KW011_002992"
     CDS             2771..3802
                     /gene="idnD"
                     /locus_tag="KW011_002992"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HBH7661275.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            3826..4590
                     /gene="idnO"
                     /locus_tag="KW011_002993"
     CDS             3826..4590
                     /gene="idnO"
                     /locus_tag="KW011_002993"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBH7661276.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            4655..5974
                     /gene="idnT"
                     /locus_tag="KW011_002994"
     CDS             4655..5974
                     /gene="idnT"
                     /locus_tag="KW011_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HBH7661277.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            6041..7039
                     /gene="idnR"
                     /locus_tag="KW011_002995"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             6041..7039
                     /gene="idnR"
                     /locus_tag="KW011_002995"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HBH7661278.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7117..8619
                     /locus_tag="KW011_002996"
     CDS             7117..8619
                     /locus_tag="KW011_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HBH7661279.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="KW011_002997"
     CDS             complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="KW011_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBH7661280.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="KW011_002998"
     CDS             complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="KW011_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBH7661281.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            11187..12698
                     /gene="pepA"
                     /locus_tag="KW011_002999"
     CDS             11187..12698
                     /gene="pepA"
                     /locus_tag="KW011_002999"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBH7661282.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            12832..13275
                     /gene="holC"
                     /locus_tag="KW011_003000"
     CDS             12832..13275
                     /gene="holC"
                     /locus_tag="KW011_003000"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HBH7661283.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            13275..16130
                     /gene="valS"
                     /locus_tag="KW011_003001"
     CDS             13275..16130
                     /gene="valS"
                     /locus_tag="KW011_003001"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBH7661284.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(16186..17382)
                     /locus_tag="KW011_003002"
     CDS             complement(16186..17382)
                     /locus_tag="KW011_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HBH7661285.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         4483 a         4336 c         4454 g         4175 t
ORIGIN      
        1 tgcgctgttg aagacgagca gccacttcaa gacgtccaga catttctacg gccttaatag
       61 gtgctaataa atctcgagtt tttaactcag cgatattacg ttcaccaagc gctgcaaaaa
      121 gattatcttc cagacttttt agcacacgat gggcatgatc ttcagaccac tttttattgg
      181 tgccatgcca ctcaatcgcg acttctttaa aggttcgtgc tttactctgt tcaaccttat
      241 cacttttctt tttgtctccc ggatcgacgc cattcgcaag cagcttacgt ggctcgtcac
      301 gacgtattct ggcatccgcg agtgtgattt caggataaac cccaagtgcc agcatctttt
      361 gctttccctc ataacggtac tgcaaacgcc agtacttaga accatttgga tggacaagca
      421 gatgcatacc gccaccatca gtcaacttat attgcttatc tgttggctta gctgttctga
      481 ctttgatgtc tgttaatgcc atgcgtattc cctccctgtt ggtacaagca ttatcgaacc
      541 ggaaatacca tcatctgtac caacatatgt tggtagaagt acgttgatat cggttgacct
      601 tgagagagtt aatatagcgg gaaaggtaga taaatactga atttcaggca taaaaaagac
      661 ctcagttgag gtctattgac atacttttgg tgccgaaggc cggactcgaa ccggcacgta
      721 tttctacggt tgattttgaa tcaactgcgt ctaccgattt cgccacttcg gcactgaaga
      781 ggtatgcgga aaacgttgtg gattatacct gtcgcacgcc accatgcaag cgccagcttg
      841 ctcgcacctc gctaagtgct gaaattttca gcattgcata cagcgatgtg taacctttgt
      901 cacactccag gcaccccgcc ctgccatgct ctacacttcc cagacaacac cagagaagga
      961 ccaaaaaatg tcgatgataa aaagctatgc cgcaaaagaa gcgggcggcg aactggaagt
     1021 ttatgagtac gagcccggtg aattgaagcc acaagatgtt gaagtgcagg tggattactg
     1081 cgggatctgc cattccgatc tgtcgatgat cgacaacgaa tggggatttt cacagtatcc
     1141 gctggttgcc gggcatgagg tgattgggcg cgtggtggcg ctcgggagcg ccgcgcagga
     1201 taaaggtttg caggtcggtc agcgtgtcgg gattggctgg acggcgcgta gctgcgggca
     1261 ttgcgacgcc tgtattagcg gtaatcagat caactgcgag caaggtgcgg tgccaacaat
     1321 tatgaatcgc ggcggttttg ccgagaagtt gcgtgcagac tggcaatggg tgatcccact
     1381 gccggaaaat atcgatatcg agtccgccgg gccgctgctg tgtggtggta tcacggtctt
     1441 taaaccactg ttgatgcacc atatcactgc taccagccgc gttggggtaa ttggtattgg
     1501 cgggctgggg catatcgcta taaaacttct gcacgcaatg ggatgcgagg tgacagcctt
     1561 tagttcgaat cctgcgaaag agcaggaagt actggcgatg ggtgccgata aagtggtgaa
     1621 tagccgcgat ccgcaggcac tgaaaacact tgcggggcag ttcgatctca ttatcaacac
     1681 cgtcaacgtc agcctcgact ggcagcctta ttttgaggcg ctgacgtatg gcggtaattt
     1741 ccacaccgtc ggtgcggtcc tcacgccgct gtctgttccg gcctttacgt taattgcggg
     1801 cgaccgcagc gtctctggtt cagcaaccgg cacaccttat gagcttcgta agctgatgcg
     1861 ctttgcggcc cgcagcaagg ttgcgccgac caccgaactg ttcccgatgt cgaaaataaa
     1921 cgacgccatc cagcatgtgc gcaacggtaa ggcgcgttac cgcgtggttc tgaaagccga
     1981 tttttgaaaa tcattcgcag cgctgatctg aggcgctacc ccctttcgca catattctgt
     2041 cttgtcgtat cgccagcacg gcctgccgac attgttcggt gacattggca atatcatggt
     2101 tgatatcaat gcgtacaata tcctgttcat ctgcttgtgg acgctccagc gcctcaaact
     2161 gactttttag taacgctacc ggcataaaat gcccagcccg ccgctgcatt cgcgcgagaa
     2221 tagtttcata gtcgccatct aaccagagga aatgaacatg ggggctaccc ttgcgtaaaa
     2281 tatcacgata ctgttttttt aatgatgaac agacaataaa tcctgtttca ttctttttat
     2341 aaagactgta tgaagcatca tttaagcgtt ccagccaggg aagtcgatct tcatcagata
     2401 atggaatacc ctgcgacatt ttatctatat ttttggctgg atgaagatcg tcaccatcaa
     2461 taaatttagc agataataac gcggcaacct tgctaccaat taatgtttta ccactccctg
     2521 aaacgcccat caaaataaag ctttcacccg ccatttaaaa tctcctgaat catcttacct
     2581 gctgcgatac tacgcctggt ggcataataa tttcacgttt ttttatttct gctgataaga
     2641 attacaaggc acatcacgtt atgcgtaaca tagtaatgta acaattttct gacgtgatct
     2701 tcatcacaaa taatgacaat taaaaccgct taaatgcttc cgaggtgtct acctgaccag
     2761 tgaggaattt atgcaagtga aaacacagtc ctgcgttgtt gcggacaaga aaactgttgc
     2821 cgttaccgag cagacgatag attggaataa taatggaaca ttagtacaaa taacccgagg
     2881 tggaatttgc ggttccgatt tacattatta tcaggaagga aaagtaggta atttcatgat
     2941 aaaggcaccg atggtgttag gtcatgaagt tatcggtaaa gttattcata gcgactcatc
     3001 agaattacat gaagggcaaa cggtagccat taatccgtct aaaccgtgcg gtcactgcaa
     3061 atactgcatt gaacataacg agaatcagtg tacagatatg cgtttttttg gcagtgccat
     3121 gtatttccct catgttgatg gtggttttac ccgttataaa atggtcgaaa cgtcgcaatg
     3181 tgtcccttat ccggccaaag ctgacgaaaa ggttatggct tttgccgaac ctttagccgt
     3241 cgcgattcat gccgcacatc aggccgacga gttacagggc aagcgagtat ttgtttccgg
     3301 tgttgggccc attggctgcc tgattgtcag tgctgtgaaa acactggggt ccgcggaaat
     3361 tgtctgtgcc gatgtgagtc cccgttccct ttcgctggcc aaagagatgg gtgcggatgt
     3421 gctcgtaaac ccacaaaacg acgacatgga tcactggaaa gcggaaaaag gccatttcga
     3481 tgtcagcttt gaagtgtccg gtcatccttc atcagtgaat acctgtctgg aggtcactcg
     3541 tgcacgcggc gtaatggtgc aggtagggat gggaggcgcg atggcagaat tcccaatgat
     3601 gacgttgatt ggtaaggaga tttcactcaa aggctctttc cgttttacca gcgaatttaa
     3661 taccgcagtg tcatggctgg cgaatggcgt tatcaatcca ctgcctttac tgagtgctga
     3721 atatctcttc actgacctgg aagaggcgct acgtttcgcc ggtgataaaa cccaggcagc
     3781 aaaagtccag cttgttttct aagagataaa taaaggaata atacaatgaa cgatctattt
     3841 tcactggcag gaaaaaatat cttgattacc ggttcagcac agggcattgg ctatttactg
     3901 gcaaccggcc tgggtaaata tggcgcacaa ataattatta atgatattac tgccgaacgc
     3961 gcagaacttg ctgtagaaaa actccaccag gagggtattc aggccgttgc cgcacctttt
     4021 aatgttactc ataaacatga aattgatgcc gccgttgaac atatcgaaaa ggacatcggc
     4081 cccattgatg tgctggtgaa taacgccggt atccagcgcc gtcatccttt tactgagttc
     4141 cctgaacaag agtggaatga tgtgatcgca gtaaaccaga ccgccgtgtt cctggtatcg
     4201 caagcggtaa ctcgtcacat ggttgaacgc aaggcaggta aagttattaa tatttgctcg
     4261 atgcaaagcg aactgggacg tgacaccatc accccttatg ccgcatcgaa aggggcggta
     4321 aaaatgctca cccgcggcat gtgcgtcgag ctggcgcgcc acaatattca ggtcaacggt
     4381 attgcgccgg gctatttcaa aacagaaatg actaaagcac tggttgagga cgaagccttc
     4441 accgcctggt tgtgcaaacg gacccccgcc gcacgctggg gagatccgca ggaactgatt
     4501 ggtgctgcgg tgttcctttc ttcaaaagcc tctgatttcg taaacggcca cctgttgttt
     4561 gttgatggcg gcatgttagt ggctgtttaa cagccaccac cctacgtgca gcgcgaaagc
     4621 gctgcttttc tggcactaat aagagataaa gactatgcca ttaatcatta ttgcggcagg
     4681 cgtcgcgctg cttcttatcc tgatgatcgg ctttaaagtt aacggcttta ttgccctcgt
     4741 tctggtagct gccgtcgtcg gatttgccga agggatggat gcacaggccg tcctgcactc
     4801 tatacaaaat ggtatcggca gcacgctcgg cgggctggca atgatcctcg gtttcggggc
     4861 catgttaggc aagctgattt ctgatacggg cgcggcacaa cgtatcgcca ctacgctgat
     4921 tgctactttt ggtaaaaaac gcgtgcaatg ggcgctagtg atcaccggtc tggttgtggg
     4981 cctcgccatg ttttttgaag tgggttttgt cctgctgttg ccgttggtat ttaccatcgt
     5041 ggcatcatct ggattacccc tgttgtatgt tggcgtaccg atggtagcag cgctctctgt
     5101 aacccactgt tttctgccgc cacatccagg gcctactgcc atcgcgacta tctttgaggc
     5161 taatctcgga acgactttac tgtatggatt tatcattacc attccgacag ttattgtcgc
     5221 aggaccgctg ttttctaaac tgctaactcg ctttgagaaa gcaccaccgg aaggcttatt
     5281 taatcctcat ctgtttagcg aagaggagat gccctccttc tggaacagta tttttgctgc
     5341 agtgatcccg gtcatcctga tggctatcgc cgccgtttgt gaaattacgt taccgaaaac
     5401 taacaccgtg cgcctcttct ttgaatttgt cggtaaccct gccgttgcgc tgtttattgc
     5461 cattgttatt gcgattttca cactgggccg acgtaatgga cgcaccatcg agcaaatcat
     5521 ggatatcatt ggggattcta taggcgctat cgcgatgatt gtgtttatta tcgctggcgg
     5581 cggcgcgttt aagcaggtat tagtagatag tggtgtcggg cagtatattt cacacttaat
     5641 gaccggaact acgctttcgc cgttattgat gtgctggact gtcgcggcgc tgttgcgtat
     5701 cgctctgggc tctgccaccg tcgcggccat taccaccgcg ggtgtggtgt tgccgattat
     5761 caacgttacc catgccgatc ccgctttaat ggtactggca actggtgcgg gcagcgtgat
     5821 cgcgtcacac gtaaacgacc ctggcttctg gctatttaaa gggtatttta atctgacggt
     5881 tggtgaaacg ttgcgtacct ggacagtgat ggaaaccctt atttctatta tgggtttgct
     5941 gggcgtgtta gccattaacg ccgtattgca ctgaaagaat aaaatattgt gatgtagttt
     6001 cgggggaaga ttgaagattc ccccgacgtt gggagaagtt atgcgcaatc acagaatttc
     6061 tttacaggat atcgctacgc tggctggcgt aacaaaaatg accgtgagtc gttatatccg
     6121 ctcgccgaaa aaggtggcaa aggaaacagg cgagcgcatc gccaaaatta tggaagagat
     6181 caattatatc cctaatcgcg ctcccggcat gttgttgaat gcacaaagtt ataccctcgg
     6241 tatcttgatt ccgtcatttc aaaatcaact cttcgctgac attctcgcgg gaatagaatc
     6301 cgtcacctct gtgcataatt atcagaccct tattgctaat tacaattacg atcgcgattc
     6361 cgaagaagag tcggtgatta acctgctctc ttataacatt gacgggatta ttctttcgga
     6421 aaaatatcac accatcagga cggtgaaatt tctgcgttca gccaccattc cggtcgtgga
     6481 attgatggat gtacagggag aacggctgga tatggaggtc ggttttgata atcggcaggc
     6541 tgcttttgac atggtgtgta ccatgctgga taagcgagtc aggcgcaaaa ttctgtatct
     6601 cggttccaaa gatgacaccc gcgatgaaca gcgttatcag gggtattgcg atgcgatgat
     6661 gctgcataat ctttccccat tacgcatgaa tccacgcgcc atctcatcca ttcatttagg
     6721 aatgcagtta atgcgtgatg cccttagcgc aaacccggat ttagacggtg tattttgcac
     6781 caatgacgat attgcgatgg gtgcgcttct gctgtgccgc gagcgaaacc tcgccgtgcc
     6841 agaacaaatt tccattgcgg gttttcatgg gctggagatt ggcaggcaaa tgatcccaag
     6901 cctcgccagc gtaattactc cccgttttga tattggtcgg atggccgccc aaatgttgct
     6961 gagcaagatt aagaataacg atcacaacca caacactgtt gacctcgggt atcagattta
     7021 tcacggtaac acgctttagg ttattttctc ttcattacac atattctctt tgccggacgt
     7081 tgcctatact ccaggcagga aactggagga aatctcatga gcgaacccct gttaattgcc
     7141 cgcacgccgg acacagaact gtttttactg ccgggaatgg ctaaccgtca cgggctgatt
     7201 actggcgcaa cggggacggg taaaactgtc acgttgcaaa aactggcaga gtcattgtcg
     7261 gaaatcggtg ttccggtgtt tatggccgat gtgaaaggcg atctgaccgg catcgcgcag
     7321 gcaggaacgg cgtcggaaaa actgctcgca agactgaaaa atatcggcgt caacgactgg
     7381 caaccgcatg ccaatccggt ggtggtgtgg gatatctttg gcgagaaagg ccatccggtg
     7441 cgggcgacgg tttcggatct ggggccgctg ttgctggcac gactgttgaa tctcaacgat
     7501 gtgcaatctg gcgtgctgaa tattatcttc cgcattgcgg acgatcaggg attgttgctg
     7561 ctcgacttta aagatttgcg ggcgattacc cagtacatcg gcgataacgc caaatccttc
     7621 cagaatcagt acggaaatat cagtagcgca tcggttggtg ccatccagcg cggattactg
     7681 tcgctggaac agcaaggcgc agaacacttc tttggtgagc cgatgctgga tatcaaagac
     7741 tggatgcgca ccgatgccaa cggtaaaggc gttatcaata tcctcagcgc cgagaaactt
     7801 tatcagatgc cgaaactgta cgccgccagc ctgctgtgga tgctttcaga gttatatgaa
     7861 caattgcctg aagcgggcga tctggagaaa ccaaaactgg tgtttttctt cgacgaagca
     7921 catctgctgt ttaacgatgc gccacaggta ctgctggata agattgagca ggtgataagg
     7981 cttattcgct caaaaggcgt gggcgtctgg ttcgtttcgc aaaacccgtc agatatcccg
     8041 gataacgtgc tagggcagct cggtaatcgc gttcaacacg ctttgcgtgc ttttacgcct
     8101 aaagatcaga aagcagtgaa ggcagcggcg caaaccatgc gggccaatcc ggcgtttgat
     8161 accgaaaagg caatccagga actggggacc ggcgaggcgt taatctcgtt tctcgatgcg
     8221 aaaggaagtc cttcagtggt ggaacgggcg atggtgatcg caccttgttc gcgaatgggg
     8281 ccggtgacgg aagatgagcg taatggcctg attaatcact ccccggtgta tggcaaatat
     8341 gaagatgagg tggaccgcga gtccgcctat gagatgctgc aaaaaggctt tcaggccagt
     8401 accgagcagc aaaataatcc ccccgtgaaa gggaaagagg tggcggtgga tgacggtatt
     8461 cttggtggat tgaaggatat tttgtttggc actaccggac cacgcggcgg gaagaaagat
     8521 ggtgtggtgc aaacaatggc gaaaagcgcc gctcgccagg tgacgaatca gattgtacgc
     8581 gggatgttgg ggagtttgct gggggggaga aaaaggtaag tcagacgtga tctgtgcccc
     8641 tcaccctaac cctctcccca aaggggcgag gggaccgttc gaactcgtat ttgactttgt
     8701 caacaataat aaagggagct ttcgctccct tgttagatta cgattttctc attaacagcc
     8761 acaggctgat taagaagaag ctggcgcttg gcaacagtgc gccgatgatc ggcgggatgc
     8821 cataaaccaa cgtcagcggg ccgaagatct ggtccagtac gtagaagaca aaaccgaagc
     8881 tgataccggt gaccacacgc acgcccatcg gtacgctacg cagtgggcca aagatgaacg
     8941 acagcgccat caacatcatc accgccacgg atagcggctg gaagattttg ctccacatgt
     9001 tgagctgata acgtccggca tcctgaccgc tcgacttcag atacttcaca tagttgtgca
     9061 aaccactgat ggagagtgca tccgggtcca gcgccaccac gcccagtttg tctggcgtga
     9121 ggttggtttt ccaggtgccg ctcaccgtct gcgaaccggt gatctgtttc ggattggtca
     9181 gatcagattc atcaacctgc gacagacgcc agactttatg ttccgggtca aacttcgcgg
     9241 tagcggcata acgtacggat tgcagacgac gattctcgtt aaaggcataa atactgatgc
     9301 cacctaactc ttcgtcacct ttaacccgct caatgtagac gaagttgttg ccatctttcg
     9361 cccataagcc ttgctgagtg gagagcaacg agccgccgta catcgcctgc gcacggtagt
     9421 tacgcgccat ctgctcgccc tgcgacgcga cccattcacc aatcgccatc gtcagcaaga
     9481 ccagcggaat ggcggttttc atcaccgaca gcgccacctg catacgggta aaaccagaag
     9541 cctgcatcac caccagttcg ctgcgctgcg ccagcatccc aagaccaagc aacgccccaa
     9601 gcagagccgc catcgggaag aagatttgca catctttcgg cacgctcagc aaggtataca
     9661 ttcctgcgcc taacgcgtcg taactcccct gcccggcttt tttcagctga tcgacaaact
     9721 tgataatgcc cgacagcgac accagcatga acagcgtcat catgatggtg gtgaaaatag
     9781 ttttaccgat atagcggtca agtacgccaa aaggttgcat cacaccgctc ctttacgcga
     9841 aaaactggcg cgcaggcggc ggaccggcac ggtgtcccaa aggttgagaa caatcgccaa
     9901 agccagataa atcaggttaa cggtccacat ccacagcgtc gggtccagct tacctttacc
     9961 gccgttcgat ttcagggagg tctggatcag gaagaaaagt agatacagca gcatggctgg
    10021 cagcatcgac agtacgcgtc cctgacgcgg gttaaccacg ctcaacggta caaccataag
    10081 tgccatcata aacacggtga ataccaacgt gatacgccag ttcagttctg cgcgggcacg
    10141 atcggtgtca gtgttccaca atgtgcgcat gtccatctgg tcggtatcgt tcgggtcgag
    10201 caccaccgcc tggtgaccaa tgatcgcctg ataatcctga aagtccgtaa tgcggaaatc
    10261 gcgtaacagt gcagtgcctt cgaagcgcgt tccctggttg agagtgacga cctgggagcc
    10321 gtcgcgcagc tgggttaaat gtccggaatc ggccaccacc acagaaggac gtgcattacc
    10381 ttttggtcga atttgtgcga ggaacacatc tttgaaatcg ctgccgtcaa cgctttcgat
    10441 gaacagcacc gagctgccat tagtcgcttg ctggaattgc ccttgcgcca gcgccgccat
    10501 gccagggttc gctttcgctt ctgctaacac ttcatcctga tgacgcgatg accatggtcc
    10561 cgcccacatc acgttaaccg ccgcgacgat tgccgtgaac accgcaagga tcattgccgc
    10621 tttcaccagc accgctttgc tcagaccgca ggcatgcatt accgtaattt cactttcggt
    10681 atacagtttg cccagcgtca tcagcagccc gaggaacagg cttaatggca ggataagctg
    10741 cgccatttcc ggcacgccca acccgagaag ggagagcacc agattcgccg gaatatcgcc
    10801 gtcaaccgct gcgccgagga tcctcactaa cttttgacag aagaagatca aaagcaagat
    10861 gaagagtatc gccagctggc ttttgagcgt ctcccgcacc agatatctta tgattatcac
    10921 tttaaatacg cccgtaaaaa ctcgtctttt gcaggatttt agcttgtttc atggcttaaa
    10981 cgtcatttat tctcttgagt cgtcgaaatc gtcgctaaga taattatact caacggattc
    11041 acctctcaga ttttgttctg acgtgccaat gccgtaataa cgttaagatt aacacgaagt
    11101 catcgcaaca gcggacatga gttacgaaag cttgcaattc tatctgtagc caccgccgtt
    11161 gtctttaaga ttcaggagcg tagtgcatgg agtttagtgt aaaaagcggt agcccggaga
    11221 aacagcggag tgcctgcatc gtcgtgggcg tcttcgaacc acgtcgcctt tctccgattg
    11281 cagaacagct cgataaaatc agcgatgggt acatcagcgc cctgctacgt cggggcgaac
    11341 tggaaggaaa accggggcag acactgttgc tgcaccatgt tccgaatgta ctttccgagc
    11401 gaattctcct tattggttgc ggcaaagaac gtgagctgga tgaacgtcag tacaagcagg
    11461 ttattcagaa aaccattaat acgctgaatg atactggctc aatggaagcg gtctgctttc
    11521 tgactgaact gcacgttaaa ggccgtaaca actactggaa agtgcgtcag gctgtcgaga
    11581 ctgcaaaaga gacgctctac agtttcgatc agctgaaaac gaacaagagc gaaccgcgtc
    11641 gtccgctgcg taaaatggtg ttcaacgtgc cgacccgccg tgaactgacc agcggtgagc
    11701 gcgcgatcca gcacggtctg gcgattgccg ccgggattaa agcagcaaaa gatctcggca
    11761 atatgccgcc gaatatctgt aacgccgctt acctcgcttc acaagcgcgc cagctggctg
    11821 acagctacag caagaatgtt atcacccgcg ttatcggcga acagcagatg aaagagctgg
    11881 ggatgcactc ttatctggcg gtcggtcagg gttcgcaaaa cgaatcgctg atgtcggtga
    11941 ttgagtacaa aggcaacgcg tcggaagatg cacgcccaat cgtgctggtg ggtaaaggtt
    12001 taaccttcga ctccggcggt atctccatca agccttcaga aggcatggat gagatgaagt
    12061 acgatatgtg cggtgcggca gcggtttacg gcgtgatgcg gatggtcgca gagctacaac
    12121 tgcctattaa cgttattggc gtgctggcag gctgtgaaaa catgcctggc gggcgtgcct
    12181 atcgtccggg cgatgtgtta accaccatgt ccggtcaaac cgttgaagtg ctgaacaccg
    12241 atgccgaagg ccgcctggta ctgtgcgacg tgttaactta cgttgaacgt tttgagccgg
    12301 aagcggtgat tgatgtggcg acgctgaccg gtgcctgcgt gatcgcgctg ggtcatcata
    12361 ttactggtct gatggcgaac cataatccgc tggcccatga actgattgcc gcgtctgaac
    12421 aatccggtga ccgcgcatgg cgcttaccgc tgggtgacga gtatcaggaa caactggagt
    12481 ccaattttgc cgatatggcg aacattggcg gtcgtcctgg tggggcgatt accgcaggtt
    12541 gcttcctgtc acgctttacc cgtaagtaca actgggcgca cctggatatt gcaggaaccg
    12601 cctggcgttc tggtaaagca aaaggcgcaa ccggtcgtcc ggtagcgttg ctggcacagt
    12661 tcctgttaaa ccgcgctggg tttaacggcg aagagtaagt ttgcgtttta ccctcaccct
    12721 aaccctctcc ctaagggaga gggaaccgat cgagcgcaat agaaattcat tgctcatctt
    12781 tcagggtgag gaatgcagca atatttaaat ccaccacaag aagccccata tatgaaaaac
    12841 gcaacgttct accttctgga caatgacacc accgtcgatg gcttaagcgc cgttgagcaa
    12901 ctggtgtgtg aaattgccgc agaacgttgg cgcagcggta agcgcgtgct catcgcctgt
    12961 gaagatgaaa agcaggctta ccggctggat gaagccctgt gggcgcgtcc ggcagaaagc
    13021 tttgtcccgc ataatttagc gggagaagga ccgcgcggcg gtgcgccggt ggagatcgcc
    13081 tggccgcaaa aacgcagcag cagcccgcgc gatatattga ttagtctgcg aacaagcttt
    13141 gcagattttg ccactgcttt cacagaagtg gtagacttcg ttccttatga agattctctg
    13201 aaacaactgg cgcgcgaacg ctataaagcc taccgcgtgg ctggtttcaa cctgaatacg
    13261 gcaacctgga aataatggaa aagacatata acccacaaga tatcgaacag ccgctttacg
    13321 agcactggga aaagcagggc tactttaagc ctaatggcga tgaaagccag gaaagtttct
    13381 gcatcatgat cccgccgccg aacgtcaccg gcagtttgca tatgggtcac gccttccagc
    13441 aaaccatcat ggataccatg atccgctatc agcgcatgca gggcaaaaac accctatggc
    13501 aggtcggtac tgaccacgcc gggatcgcta cccagatggt cgttgagcgc aagattgccg
    13561 cagaagaagg taaaacccgt cacgactacg gccgcgaagc tttcatcgac aaaatctggg
    13621 aatggaaagc ggagtccggc ggcaccatta cccgtcagat gcgccgtctc ggcaactctg
    13681 tcgactggga gcgtgaacgc ttcacgatgg acgaaggcct gtccaatgcg gtgaaagaag
    13741 ttttcgttcg tctgtataaa gaagacctga tttaccgtgg caaacgcctg gtaaactggg
    13801 atccgaaact gcgcaccgct atctctgacc tggaagtgga aaaccgcgaa tcgaaaggtt
    13861 cgatgtggca catccgctat ccgctggctg acggcgcgaa aaccgcagac ggtaaagatt
    13921 atctggtggt tgcgactacc cgtccggaaa ccctgttggg cgataccggc gtggccgtta
    13981 acccggaaga tccgcgttac aaagacctga ttggcaaata tgtcattcta ccgctggtta
    14041 accgtcgtat tccgatcgtt ggcgacgaac acgccgacat ggaaaaaggc accggctgcg
    14101 tgaaaatcac tccggcgcac gactttaacg actatgaagt gggtaaacgt cacgcgctgc
    14161 cgatgatcaa catcctgacc tttgacggcg atatccgtga aagcgcccag gtgttcgata
    14221 ccaaaggtaa cgaatctgac gtttattcca gcgaaatccc ggcagagttc cagaaactcg
    14281 agcgttttgc tgcacgtaaa gcagtcgttg ctgcggttga cgcactcggc ctgctggaag
    14341 aaattaaacc gcacgacctg accgttcctt acggcgaccg tggcggcgta gttatcgaac
    14401 caatgctgac cgaccagtgg tacgtgcgtg ccgatgtact ggcgaaaccg gcggttgaag
    14461 cggttgagaa cggcgacatt cagttcgtac cgaagcagta cgaaaacatg tacttctcct
    14521 ggatgcgcga tattcaggac tggtgtatct ctcgtcagct gtggtggggt caccgtatcc
    14581 ctgcatggta cgacgaagca ggtaacgttt atgttggccg caacgaagaa gaagtgcgca
    14641 aggaaaataa cctcggtgcc gacgttgccc tgcgtcagga cgaagacgtt ctcgacacct
    14701 ggttctcttc tgcgctgtgg accttctcta ccctaggctg gccggaaaac actgacgcac
    14761 tgcgccagtt ccacccaacc agcgtgatgg tatccggctt cgacatcatt ttcttctgga
    14821 ttgcccgcat gatcatgatg accatgcact tcatcaaaga tgaaaatggc aaaccgcagg
    14881 tgccgttcca caccgtttac atgaccggcc tgattcgtga cgacgaaggc cagaagatgt
    14941 ccaaatccaa gggtaacgtt attgacccgc tggatatggt tgacggtatt tcgctgccgg
    15001 aactgctgga gaaacgtacc ggcaatatga tgcagccgca gctggcggac aaaatccgta
    15061 agcgcaccga gaagcagttc ccgaacggta ttgagccgca cggcaccgat gccctgcgct
    15121 tcaccctggc ggcgctggcg tctaccggtc gtgacatcaa ctgggatatg aagcgcctgg
    15181 aaggttaccg taacttctgt aacaagctgt ggaacgccag ccgctttgtg ctgatgaaca
    15241 cggaaggtca ggattgcggc ttcaacggtg gtgaaatgac gctgtcgctg gcggaccgct
    15301 ggattctggc agagttcaac cagaccatca aagcgtaccg cgaagcgctg gacagcttcc
    15361 gcttcgatat cgctgcaggc attctgtatg agttcacctg gaaccagttc tgtgactggt
    15421 atctggagct gactaagccg gtaatgaacg gtggcaccga agccgaactg cgcggtactc
    15481 gccatacgct ggtgaccgta ctggaaggtc tgctgcgcct cgcgcatccg atcattccgt
    15541 tcatcaccga aaccatctgg cagcgtgtga aagtactttg cggtatcact gccgacacca
    15601 tcatgctgca gccgttcccg cagtacgatg catctcaggt tgatgaagcc gcactggccg
    15661 acaccgaatg gctgaaacag gcgatcgttg cggtacgtaa catccgtgca gaaatgaata
    15721 tcgcgccggg caaaccgctg gagctgctgc tgcgtggttg cagcgcggat gcagaacgtc
    15781 gcgtaaatga aaaccgtggc ttcctgcaaa ccctggcgcg tctggaaagc atcaccgtgc
    15841 tgcctgccga tgacaaaggt ccggtttccg ttacgaagat catcgacggc gcagagctgc
    15901 tgatcccgat ggctggcctc atcaacaaag aagatgagct ggcgcgtctg gcgaaagaag
    15961 tggcgaagat cgaaggcgaa atcagccgta tcgagaacaa gctggcgaat gaaggctttg
    16021 tcgcccgcgc accggaagcg gtcatcgcga aagagcgtga gaagctggaa ggctatgcgg
    16081 aagcgaaagc gaaactgatt gaacagcagg ctgttatcgc cgcgctgtaa tcaaaacaca
    16141 gtgatgaaaa cgaaggccgg agcatgctcc ggcctttttt atctcttaca gaaatggtaa
    16201 agaaggcata attccccgtg aaatactggc taagaatcct ttatctggtt gttcttcttt
    16261 attgattaaa ggaaggtcat ccaaatcgcc cagcaaatac gtattttttg cctgccagtc
    16321 aatcatccat attttcagca gtggataagc cagaccgccc gtaatcccgg atatcaccac
    16381 caacgcacac atgcgataaa gcataccgtg gtatgttaaa gttgagcgaa aacgaatacg
    16441 cccatcattc agtgacaggt tgctcataaa atgatttcgc aacgacaccg ttaaataact
    16501 ggtgctaaca gcaatcccaa aataatagat taactgcgcg attatcattt tacgttgcat
    16561 ttccataaat tgctgtaaat tctcaatatc atcatttgca tacccagaac tatcatactc
    16621 atttaatatt tgatcgaaga taatataacc agcaacagcg agaaaaggta ataaagccaa
    16681 aaatgcgaga atggcatatt ttatacaata tgtagtatct aatttcacct tgaaacgatg
    16741 tataccgaaa ctggtattac tccagagaaa actcattacc agactatata aagtgccatt
    16801 aaaaatacca atgaaaacaa tacaaactat tgtcatcaga accacagata taataacgct
    16861 acttgaacta ttggcatgta gcatctttgt cgagataaag aaaacagtcc ccatcccaag
    16921 ggccattaaa atcggcaaga aaaaggtcac ccaccagaac cctttcatag agcaattaaa
    16981 actaaatctt acgccgttga gactggtcat caaggcctga taacgcagtc cttttgctgc
    17041 cataaatata agcaaaacca acagtgacaa agtcaaaaca cagccaataa gcggcatatc
    17101 tgctgacact gtcattaaga ttgcgaaata gaaaaaaaca aaaacaagac aactaacaaa
    17161 aacattccca ccggtaatcc cataagaaaa tcgttgtccg ttaacttcca tattagcata
    17221 gagataacgc ttacatttca ttaatgccca tggtaaatag atacctagcg ttataatcgt
    17281 taacaacaca ttcaccacac aaataagaaa atatctctca cctgtaccat gaaaaacaaa
    17341 cgaatgggac ggaacatcca tatcattaat aacttgagcc ataaaagttc cctgaattaa
    17401 aaaatccatt gatataattt atatatacag acagtaaaat gttctgcc
//
LOCUS       DAESHI010000087        17338 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.088, whole genome shotgun sequence.
ACCESSION   DAESHI010000087 DAESHI010000000
VERSION     DAESHI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17338
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.088"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            92..1132
                     /locus_tag="KW011_003003"
     CDS             92..1132
                     /locus_tag="KW011_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HBH7661286.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="KW011_003004"
     CDS             complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="KW011_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HBH7661287.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2350..4509)
                     /gene="aas"
                     /locus_tag="KW011_003005"
     CDS             complement(2350..4509)
                     /gene="aas"
                     /locus_tag="KW011_003005"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HBH7661288.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5095..6126
                     /gene="galR"
                     /locus_tag="KW011_003006"
     CDS             5095..6126
                     /gene="galR"
                     /locus_tag="KW011_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HBH7661289.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="KW011_003007"
     CDS             complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="KW011_003007"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBH7661290.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7517..8452
                     /gene="lysR"
                     /locus_tag="KW011_003008"
     CDS             7517..8452
                     /gene="lysR"
                     /locus_tag="KW011_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HBH7661291.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8439..9131)
                     /gene="ygeA"
                     /locus_tag="KW011_003009"
     CDS             complement(8439..9131)
                     /gene="ygeA"
                     /locus_tag="KW011_003009"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBH7661292.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(9262..10680)
                     /gene="araE"
                     /locus_tag="KW011_003010"
     CDS             complement(9262..10680)
                     /gene="araE"
                     /locus_tag="KW011_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HBH7661293.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(10995..11756)
                     /gene="kduD"
                     /locus_tag="KW011_003011"
     CDS             complement(10995..11756)
                     /gene="kduD"
                     /locus_tag="KW011_003011"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HBH7661294.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11821..12657)
                     /gene="kduI"
                     /locus_tag="KW011_003012"
     CDS             complement(11821..12657)
                     /gene="kduI"
                     /locus_tag="KW011_003012"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBH7661295.1"
                     /translation="MDVRQGIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(12944..14125)
                     /locus_tag="KW011_003013"
     CDS             complement(12944..14125)
                     /locus_tag="KW011_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBH7661296.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            14380..15609
                     /locus_tag="KW011_003014"
     CDS             14380..15609
                     /locus_tag="KW011_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBH7661297.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            16069..16701
                     /locus_tag="KW011_003015"
     CDS             16069..16701
                     /locus_tag="KW011_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH7661298.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            17035..>17338
                     /locus_tag="KW011_003016"
     CDS             17035..>17338
                     /locus_tag="KW011_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH7661299.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVTQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPREDS"
BASE COUNT         4597 a         4523 c         3988 g         4230 t
ORIGIN      
        1 ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc
       61 cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc
      121 gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc
      181 cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc
      241 cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg
      301 caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg
      361 accgtcgcgc aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat
      421 ccgcgaagcg ctgcatgaca gcctcaagcg cctacacact gattacctcg atctttatca
      481 ggtgcactgg ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga
      541 ttctgcgcct gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgcgc
      601 gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct
      661 gcatctggcg gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct
      721 gttaaaccgc agttttgaag taggtctggc agaagtaagc cagtatgaag gggtcgaact
      781 gctggcctat tcgtgcctgg gtttcggcac gctgaccggg aaatatctca atggtgcaaa
      841 acccgctggc gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac
      901 gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catggcctag accctgctca
      961 gatggcgctc gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac
     1021 cacgatggat cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt
     1081 attagctgaa attgaagcgg tgcatcaggt ttatacttat ccggcaccat aagattaacg
     1141 ccggttaaaa ccggcgttaa atattaatga cggcgctgcc agatccacag cgccgttatt
     1201 gccagcgcaa acagcgcacc gaagccaatg ccaatgggca cgaccgggat gcctaccatt
     1261 accgccagcg agtaaatgcc cagcatcaac aacatggcgc tgttttcgcc aaggttttgt
     1321 actgcaatcg cattccccgc cccgacgctt tttttacccc gctcctgtag caacgcattg
     1381 agcggaacga caaaaaagcc ccccatcacg ccaatcagca tcaacaaggc ataggctggc
     1441 agcagctcgt gttgcaggga aaaaatcagt accaccacgc caatcaaaat cccggctggc
     1501 atacagcgtg acacggtttc cagcgtaact aacttcgctg ccgcacctgc gccaaccacg
     1561 ataccaatcg ctaccatcgc gttgagatag gtgggcgtag cgttatcggt aatgcccagc
     1621 gccaccggta cccacagcac caacaggaaa cgcagcgtga cacccgctcc ccagaataaa
     1681 ctggtgccca ccagcgaaaa acgcgtttca ccattgcgcc atagcgaggt gcaggcattc
     1741 aggaaactgc gggtcatgtt gatgagattc caggactgcc ccggacgcgc cgccgccagt
     1801 ttgggaatgt agatattggc aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg
     1861 gcgacgagga catgccagtc agccagcaca ccaccggcta cggaaccgag caaaatcgcc
     1921 gctatggtag aagcttccat taaaccgtta gctttcacta acttactacc cgtggttaat
     1981 tcgccgagaa taccgtattt cgccggtgaa taggctgcag caccaacacc caccagcgta
     2041 tagccgagaa acggattgat accaaagcag atactggctg cgcccagcag cttcaggccg
     2101 ttggcaaaca tcatcacccg gcctttggcg aagctatccg ccacctgccc gacaaacggc
     2161 gcaaaaagaa tgtaagcacc tacaaacacc atttgcagga tgggctggct ccactccgga
     2221 tagaactgcg ctttcagtaa cgccagagtg gcaaacagta gggcattatc gccaaacgca
     2281 gagagaaact gcgccacgat aaccgctttc atccccttcg accacaacga agtgttagtg
     2341 tgcactgact cactcatcgt gttgttccgc ttcgtctacc cagcttttca acgtgacaaa
     2401 gtcaggtttg ccgctgccaa gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac
     2461 agcaagctcc ggcacgccgt gctcgcgggc atactgttgc aacttatcgc gcgtcagttc
     2521 gttatctgtg gtgaaaagca ccagtgcctc gcctttgctg gcatcgctct taatcgcagt
     2581 ggcatggact ttatctggcg aaacaccaag tgccagttgt tccaccattt ccagcgacac
     2641 catttcgcct gcaattttgg caaagcgttt tgcgcggccc tgaatctgca caaagccctg
     2701 ctcgtcaaaa cgcacaatat cgccagtgtc ataccagccg cgctccattt cgccgcgaac
     2761 attctcggcg gtgggcactt ccagtacacc tggcttctcc acccgcagat agccgttcat
     2821 tatgttcggc cctttcagtt gcaggcgtcc gccctcttcg ataccaggga ccgacaacag
     2881 gcgcgcatcc attcctggta gaatacgccc taccgtaccg ggtttcgccg ccatcggtac
     2941 gttgatagaa acgacaggcg cgcattcggt cacgccgtag ccttcaagga tgcgcaggcc
     3001 aaatttatcc tgccaaagct gtttggtact ttcttgtaat ttttctgcgc ctgccaccac
     3061 atagcgtaga cgatagaagt catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt
     3121 cgaggtgccg aacaacacgg tgcaactgcg gtcatacacc aactccggca caatacggta
     3181 atgcagcggg cttggataaa gaaacacttc tgcacctgtg agcagtggcg taaacaggcc
     3241 taccgtcagc ccaaaggagt gaaacagcgg taacgccgac ataaagcgat cgttggtggt
     3301 gaagtcggca atcgttttaa tctgctcgac gttcgccaga atgcttttat ggctatggac
     3361 gacgcctttc ggatggcctt cagaaccgga ggtaaagagg atcagcgcct cttcttccgg
     3421 ctgctgtttt acctgtgcca gacgtggcat cagcagatgc gcgaagatcc atactttgtc
     3481 ggcagtggtg acatctgctt ttaaatcttc cagatagacc cagcgcacct gagtaagttg
     3541 ctccggcaga tgccagagtt tgcctttatc gagaaactgg cgggaagtga agatggtttt
     3601 gatttcagcc gccgtaatag cactggtcag cccttttacc ccggcagtgt agttcatcat
     3661 tgcgggaatg cggcgacggg cgatggcccc aaaaatcact gccgcactga tgcctgcatt
     3721 gggcagcatt aagccgatgc gttcgccttc aacactgtat ttttcaagga tgcgtccaac
     3781 aaacagcgtt ttcgtaagca atttgcgata ggagtctggg gtaaagttga cgtcttcgac
     3841 acatttcttc ccggctccga agcggtacat tgcactcagt aaagattcgt acagcgtttc
     3901 acgcgggcgc accgccattc gcgcttccat cattatttga tgcagcattt cgccagcgat
     3961 tttgcgacgg tcacgggcac gcggcgcatc cggcatctcc acctgcgttg gtggcaaaat
     4021 atgcagagta atttgcggga acaagcgacg tttaaccaga cctttcaggc ggctgaagtg
     4081 cgtaagttcc gccccttcaa tacgcacagg aataaccgtt gcaccagact tcgccgcgac
     4141 aaaacccgcg ccatcgtaga ttttcatcag cgagcctgtg gtggtgatgc gtccttcagg
     4201 gaaaatcacc actggtcggc cctgttccac cagacgtacc agatgtttaa tcgccatagg
     4261 ttgcgtcggg tcgagaggaa caaagtcgat aaatgatttc agccaacgca tataccactg
     4321 ttggcttatt gaggtgtaaa cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa
     4381 aatgccatca ataaaagaga cgtgattagg cgtaattaga acgcgctcgc ccttcagtgc
     4441 ctgggtgtca cccgtaacgc gaacgcgata caaaacacgg cacaaatttc gaaaaaagct
     4501 aaaaagcatt ccaactccct ttgctctgat tcagtaaaag cgaatggagg gagattacac
     4561 gagataaaga acgcgagcga cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca
     4621 ggttggtgca agagacaggg tacgaagagc gtaccgaata atctcaccaa tcaatacctc
     4681 tgggatcttg attgtggtct gcacgacgac tcttcgccag cgagaaaacg caaaggaatg
     4741 aagggaaatg caacgaggtg tgtaaattgt cggttactgt tacagattga tgaccggcaa
     4801 aaaaaaacct gcgcatctgc gcaggctggt gtaattcatg tgctcaaccc gaagttgact
     4861 tcacctatca atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagca
     4921 gtcaatcgca acagcatttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct
     4981 tacttatttg cgtttggctc acattcccac gatgaaaaca cgccgcccct tgaaccaacg
     5041 ggcgttttcc gtaacactga aacaatgtaa gcgtttaccc actaaggtat tttcatggcg
     5101 accataaagg atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt
     5161 aataattcac ccaaagccag cgaagcgtcc cggctggctg tgcatagtgc aatggagtct
     5221 cttagctatc acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc
     5281 ggtctggtcg ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa
     5341 caggtggctt atcacaccgg taatttttta ttgattggca atggttacca caacgaacaa
     5401 aaagagcgtc aggccattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat
     5461 gccaaaatga tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg
     5521 ctgatcaacc gtatcctgcc tggcttcgaa aaccgttgta ttgctctgga cgatcgttac
     5581 ggtgcctggc tggctacgcg tcatttaatt caacaaggtc atacccgcat tggttatctg
     5641 tgctctaacc actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt
     5701 gctgaaagtg gtattgcggc caatgaccgg ctggtgacat ttggtgaacc agacgaaagc
     5761 ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc
     5821 tgttataacg attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat
     5881 gtaccgggtg agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt
     5941 ccgcgcctga ccaccgtgcg ttacccaatc gtgacaatgg cgacccaggc tgccgaactg
     6001 gctttggcgc tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg
     6061 ctggtacgtc gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc
     6121 gactaaccgc aattaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg
     6181 aatcaaccgc gcctgaccat tatcaaacag aacttctggt aacagcggac ggctgttgta
     6241 gttggatgac attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacccgc
     6301 cttcacttcc ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc
     6361 gcccgattca cataatggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga
     6421 acgaccatca gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat
     6481 cagatcgttg aacccggcat caaccagcac aaagtggcgg ctacccattt gtttgacgct
     6541 ccgcacctga gtaattaaca cgccagactg cgctaccaga aagcgacccg gttcaatttc
     6601 cagtttcaca gggtggccca aatggcgagc gatttgctca cgcgcggcat tccacagacc
     6661 ataataatgt tcggtatcaa ccgcctcttc accctgttga taaggaatag aaagcccacc
     6721 gcccgcagaa atagcctgta aatcctgacc gaattcgatg acctgacgta ccatagcacc
     6781 acacacctgt tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac
     6841 cagctgcaga tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat
     6901 accgtgcttg ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc
     6961 cgggttaacg cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat
     7021 atcaacagaa cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg
     7081 atcgataaca tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc
     7141 cgccaacgca cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg
     7201 cattaatcgc aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa
     7261 ctgtttcagc gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca
     7321 gccaaattca gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa
     7381 cagtgaatgt ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata
     7441 aaaagaataa aaaatatcga tttatgtcga gtctatgcaa aaatgatata gattaccgga
     7501 ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt
     7561 aatgaccgcc ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt
     7621 cagccgcgaa ctggcgcgct ttgagaaggt gatcgggcta aaattgtttg agcgcgtacg
     7681 tggacgatta catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg
     7741 gtacggactg gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga
     7801 actgtctatt gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc
     7861 ctttctggca cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact
     7921 tgaagagtgg ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc
     7981 tgcgggaaca gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg
     8041 tcatccgctg gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat
     8101 cagcctttcc cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca
     8161 ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg
     8221 ggcgggggta ggtgtttcgg tggttaaccc actcaccgca ctggattatg cggcaagcgg
     8281 tttagtggta cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct
     8341 gcaccgcccg tcatcagcgc tggttcaggc gtttagtgag catttacaag cggggttacc
     8401 gaaactggtc acttctcttg acgcgatttt gtcgtcagct acgacagcat aaaagcgaca
     8461 gcatcctcgg catggatcgc cgcagtatca aacacaggca aaacactgcg ctcttctgga
     8521 accaataaac caatttctgt gcagccaaaa atgacaccct gcgccccctg ttctgcaagg
     8581 cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt
     8641 tcttcaaaaa taatctgatt aatcttcgcc cgttcatccg cttccggaat aagacaattg
     8701 atggaaaatt gttccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta
     8761 cccagcagcg ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgca
     8821 atgtgtaaga aaggcagagt gcaacgtgac tcaatggcat ccgccacttt atgcatcgta
     8881 ttggtacata acacaatacc ttctgcgccc gcccgctgta agccaagcgc cgcctcagcc
     8941 agaatgtccc cggttttatc ccattccccg cgacgctggc actcttctat ttcatgaaaa
     9001 tcgacgctat gtagcagcac ttgcgcagag tgaagcccac caagccgctg tttaatgcct
     9061 tcatttatca gacgatagta aggaatggtg gattcccagc tcattcctcc cagcaaacca
     9121 attgttttca ttcctctctc tcctgatgtg tgttaccgca acaaggctga ataaggaacg
     9181 agacaaacgc ctcaacggcc aggtgcccaa tctctattaa cgaaaaaagg gccggatgta
     9241 cagcacatcc ggcccgtgaa atcagacgcc gatatttctc aacttctcgc ctgccatcag
     9301 tttgcgttcg atatgttcca gcgtgacatt tttggtttcc ggaatgagcc agaaagtgat
     9361 gcccacaaac gcaatgttca gcgcagtgta gagccagaac gtaccggcag caccaatgct
     9421 gtcgagcagc gtcaggaagg tcgcgccgat aatcatattc gacacccagt tcgtggtggt
     9481 cgaacaggta ataccgaaat cgcggcattt cagcggctga atttcagagc acaggatcca
     9541 caccactggc gcggcgctca tcgcataacc ggcaatacac atcatcgtca tgccaacaga
     9601 gagccaggac aagccactgg aagccgtacc gttatcaaac tgcatcaggc aatagcccag
     9661 caccagagtg cctaacgcca tcacgctgaa accaattttc agagccggtt tacgccctgc
     9721 tttatctacc gtaaacaccg caataaaggt ggcgaacata aaggtcagcc ctacgaccag
     9781 agtcgcaatc atctgttgtt ctgtggtcgt aaagcccgcc attttgaaga tacgcggcgc
     9841 gtagtacatg atgatgttca taccggtaaa ctgctgcatc gcctgcaaca acataccgag
     9901 aaacacagca cgacggacgt tacggttgat cttaaacagt gcccaaccgc cctgttttaa
     9961 cttcaggctt tcacgaattt cgttgagttc ttctcgcgct ttttccgacg tatcgcgcag
    10021 catacgcaac acttcttccg cctcaatatg acgccccttt tccgccagcc agcgcgggct
    10081 atttggcagg aagaccacca gaataatcag caggactgct ggtaaagcaa gaacccccaa
    10141 cattgcgcgc cagttaccgc tataactgaa cgctgtatcg gataaaaacg ccagcacgat
    10201 gccgagtgtg accatcaact ggtacatact gatcatctta ccgcgaacgt tttcacttgc
    10261 catttcagaa agatacagag gagcggtgta agacgcgatc ccgacagcaa tgcccagcac
    10321 cacccgagcg gcgattaaca tctctacgct ggtcgcaaaa gcggacccta tagaaccgag
    10381 tacaaacagg atggcccccg ccatcaggct gtatttacgc cccaggcgga acgacagcca
    10441 accattaaac agcgcaccaa ttgctgcgcc gagcatcatg ctactaacca cccattcctg
    10501 caaacgactg gtcagcacaa agtgatcggt aatgaacggc aacgctccgg cgattacgcc
    10561 gatatcaaga ccaaataaca atcctgcgac cgcagcagct accgaaacaa acatattcat
    10621 acgccgcgta tcccgcaaag aacgtggcgt taaagcagat tccgtattga tagtaaccat
    10681 tttttcctgc cagcagagag taagacatag tgaaaaaata cgtgaacaac tcacacaggt
    10741 gtcaggtcgg aaacagcata aatatggatt aaattgctgc gacatgtcgt tatgtgatgg
    10801 atattccaat tttcaaatta agttgaatta ttgagattat tattaaccac ctaattttac
    10861 agcagataaa attcataaag ttcattaatt gataattgat atggattatt tcataaccat
    10921 gatatgaatt atgatgacct acaggtataa aaaaccctgc catgaggcag ggtcataaaa
    10981 gtaagaggaa tgaattaacg agccagccaa ccgccatcta cggcaatggt ataaccattc
    11041 acataatctg aagcgctgga ggcaaggaac actatcggcc ccatcaggtc actcggcagt
    11101 ccccaacgac cagctggaat gcggtcgaga atttccgcgc tacgttgttc atctgcccgt
    11161 agttgttgag tattgttggt cgccatgtaa cccggagcta tcgcattaac attaatgttg
    11221 tgtttagccc attcgttcgc catcaatcgc gtcacgccca tcacgccgct ttttgatgcg
    11281 gtataagaag gcacacggat cccgccctgg aaggagagca ttgacgtgat attgataatc
    11341 ttgccgccat tgcgttgcgc gataaagtgt ttcgccgctg cctgagacat gaagaatacg
    11401 ctcttgatat tcaggttcat gacatcgtcc cagtcttttt cgctgaactc gagagcatct
    11461 tcgcggcgaa tcaatccggc gttattcacc aggatatcaa tatgaccaaa ctcagctacc
    11521 gcgcgatcca gcagtgctgg aatgccatca atctttcgca gatcggcggt caggcttaaa
    11581 aaacgacgcc ccagcgccgt gacctgcttg atggtttcag tcggttcaac gatgttaatg
    11641 ccaacaatgt cacagcccgc ttgcgccagc cccaacgcca tcccctggcc cagtccagta
    11701 tcacaaccag tgacgaccgc aactttacct tcgagagaaa atgcacttaa aatcataaca
    11761 ataccttatt cgtttatgcc gacaactagc gcaaatcttt aacggccact atgccgacaa
    11821 ctagcgcaaa tctttaacgg ccacatggtc catatcatca aagacctggt tttcaccaac
    11881 catgccccag ataaaggtat aagctttggt tccgacgccg gaatggatcg accagctggg
    11941 ggagatcacc gcctgctcgt tatgcatcac aatgtgacgc gtttcttgcg gctgccccat
    12001 catgtggaaa acgcaggcgt catcatccat attgaaatag aaataaactt ccatccggcg
    12061 ctcgtgagtg tgacacggca tggtgttcca caagttacct ggagccagct ccgtcagccc
    12121 catactcaat tggcaggttt ccagcacatc cgggacaaaa tatttgttaa ttgtgcgacg
    12181 gttactggtg aggttatcgc ctaacgtgac tggagatact tcgtccggtg tgactttttt
    12241 ggtgggatac gtcgtatgcg cgggtgcgca attgtaataa aactttgccg gagtggcggt
    12301 atcaatactg gcaaagacaa cttcttttgc acctttacca acatacaggg cgtcgcggtg
    12361 accgatttca tagcattggc catcgacagt aatcgtaccg gcaccgccaa tattgataac
    12421 acctaactcg cgacgttcaa ggaaatagct tacgccgagt tgtttaccaa cttccccgcc
    12481 aacggaaacc gttttagtta tcggcataat gccgccaaca ataattcggt caatgtggct
    12541 gtaaaccatg gtgtactcat cggcgacaaa taccttttca accaaaaatt cattgcgcag
    12601 cccttgggta tccagcgttt ttgcgtgcgc actgtggatg ccctgtctta cgtccacatc
    12661 aacctccgat aatcagtgaa gcgtgaaaat agaaaacgat agtgaaacac tgtttcgttt
    12721 tctctataaa gggatgatat gcccatgggc tagggttttc aattaaaaat aaaacaatgt
    12781 ttcattttta agttaaggat taaaaaaagt gcagtgtcga tcacgaatat gtcatgagaa
    12841 tggaaaaatg agggagccca gaaaatgtat gtcgacttgc cagccatcat tattcaacag
    12901 attaatgacg gcagatattt atcggtaagg aggaaatgca gagctactcg tcacgttcaa
    12961 tggtcaatgc cacgccctgg cccccgccga tacaaagcgt tgccagccct ttgcgggcat
    13021 tacgtttcac catttcatga accagagaaa ccaggattcg gcaaccggaa gcgcctatcg
    13081 ggtgaccgag tgcgatcgcg ccaccattga cattgacccg acgctcatcc cactcaagca
    13141 tcttgccaac cgaaagcgcc tgtgcagcaa acgcttcatt agcctcgata agatcgacat
    13201 cagccaactg ccagcctaca cgctccaggc aacggcgggt cgcatacacc ggcgcaattc
    13261 ccatcaatgc cggatctaca ccaacgctgg caaatgcgcg gatgcgggcc agcacgggta
    13321 aattcaacgc tcgtgctttg gcttcgctca tcatcattac cgcagctgcg ccatcgttta
    13381 tggatgatgc attccccgcg gtcacagaac cgagactatc aaatgaagga tttaaacggg
    13441 ctaaaccttc tgcactggcg tcagtgcgtg gctgttcatc ggtatcaaca accaacgtct
    13501 gaccgttact ttggatcatt accgggacga tctcatcttt aaatcgtccg gcgtcaatcg
    13561 ccgctcgcgc tttttgttgc gagctaagtg cgtaagcatc ctgcaactga cggctgatgc
    13621 catattcgcg agccagattt tcggcggtga caccaatatg ataatcattg aaggcatccc
    13681 acaacccatc atgcaccaga ctgtcaacca actggctatt gccaagctgt gcaccggtgc
    13741 ggctatcagt cagaacatgt ggtgcgcggc tcatgttttc ctggccaccg gcgatgacaa
    13801 tatcagcctc gccacactgt atcgcctgag tagccagatg cagtgcttta agtccggaac
    13861 cgcaaacgtc attaatcgtg attgcagaaa cgctattagg aagaccacct ttaatagccg
    13921 attgccttgc cggattctgc cctgcccctg cagtcaacac ctgaccaaga attacttcat
    13981 ccaccgcata tgcaggaacg ccggtacgtt ctattaacgc tttcacgacc agactaccaa
    14041 gttccacggc ggaatgaccc gctaacgcac cacgaaagca gccgataggt gtccgtaacg
    14101 ccccgacaat cacaacgtct ttcatcacaa cctcgcacca attaacagtg caatagtaaa
    14161 tgattgttaa ttacagttat cttaattgtt taaaaaaagt gatttttatc acaaaggaaa
    14221 tatgcctgag cagtagtcag agtcataact ggcacgtaag gtttgcaacc actaacccac
    14281 caatagatgg gtagataagg cgttaatctc ccatacttaa cctggtttat ggtaaattgc
    14341 cctccatttt gtttaatttg tagatgatac gttcagataa tgtctaatat ttggtctaaa
    14401 gaagaaactc tgtggagttt cgcgctctac ggcacagccg ttggtgcagg cacgctcttc
    14461 cttcctattc agttaggttc ggcaggggct gtggtcctgt ttattactgc tctggtcgcc
    14521 tggcctttaa catattggcc acataaagcc ttatgccagt tcatcctctc atcaaaaaca
    14581 tcagcatgtg aagggataac gggcgcggta acacactact atggcaagaa gattggtaat
    14641 ctgattacca cgctgtactt catcgccttt tttgtcgtcg tgttgatata tgcagtggca
    14701 attaccaact cacttacgga acagctggca aagcatatgg ttattgatct tcgcatccgt
    14761 atgttggtga gtctgggtgt tgtattaatt ctgaatctca tttttctgat gggacgtcat
    14821 gccactattc gggtaatggg atttttggta ttcccattga ttgcctattt cttatttctt
    14881 tccatttacc ttgtcggtag ttggcaacct gatctattaa cgacccaggt agagttcaat
    14941 cagaataccc ttcaccagat atggatatcg attcccgtga tggttttcgc ctttagccat
    15001 acgcccatta tttctacgtt tgccatagac agacgtgaaa aatatggcga acacgctatg
    15061 gataaatgca aaaaaattat gaaagtcgct tatctcatca tctgcataag tgtactgttc
    15121 tttgtcttta gctgcctgct ttctattcca ccttcgtata ttgaagcggc taaagaagaa
    15181 ggggtcacca ttttatcggc gctttctatg ctgccgaacg ccccagcatg gttgtcaatt
    15241 tccgggatta ttgtcgcagt agttgcgatg tcgaaatcat tcctgggtac gtactttggc
    15301 gttattgaag gtgccacgga ggtcgtcaaa acaacactac agcaggttgg tgtaaagaaa
    15361 agtcgtgcat ttaaccgcgc actatcaatt atgttggtat cgctgattac cttcattgtt
    15421 tgttgcatta acccgaacgc gatttcgatg atttatgcga tcagcggccc gctcattgcc
    15481 atgatacttt tcatcatgcc tacgttgtca acgtatctga tcccggcgct taaaccctgg
    15541 cgctccatcg gaaatctgat tacgctgatc gtgggtatcc tgtgcgtatc ggtaatgttc
    15601 tttagctaat atccgttgtg gatggttcaa aattgctaca attttgaacc attccatttt
    15661 tcataatatt catttgttat tattccgccc tatcacttct ctcgtcatat cctagaatta
    15721 tcagttagtt aaacacacaa agagatctga acatcttaaa taagactatt taagatgcat
    15781 aacttagatt cgcaagatat cttctggtca ttcaaaaaca atttgcgata tagatattct
    15841 cataccatga gatagttatc tggaccacgt tcgaccatgc aagattaaat cagtgaaaca
    15901 taatcatatt taataacgag aaatgcattt ttaaatgtct taatcagaat tatagcaaat
    15961 acagattaat cgcaattttt cccagcaaaa ttacaaaatc aagatgtatt gacatcatca
    16021 atcaccaatg aaattatgca attatatacg gatagggagg ttcttaacat ggggctttgt
    16081 agtcgttata aaagtcttac atgcaatagt tgctcaatgc attgccaaat aatgccagaa
    16141 gagtcaccgc gtttgcagta ttgcgcaaac tcgtgttttt gtatgtggcc cgaagaaagt
    16201 tcatatttta atcgtggtgt ggtggaaggt atcttaacaa aaaaccacaa tgcgagatta
    16261 agcggatata tttttgtgga tttttcagta agttttttac gtctattcct tgaaaaagac
    16321 tggattgact atcttgcaag tactgacatg gggatagttt tagtcagcga ccgcaatatg
    16381 caatcgttag ctaactactg gcgaaaacac aacaccgcaa tatctgctgt catatataat
    16441 gatgatggtc ttgatgttgc caatgaaaag atcagacaac tatttattgg tcgttattta
    16501 tcatttacca gaggtaacac attaacacag atggaattca ccataatggg gtatatggtt
    16561 tctggttata atccatatca gattgctgaa gtgctggata tggatatccg tagcatctac
    16621 gcgtacaagc aacgaatcga aaagagaatg ggtggtaaaa taaacgaatt atttattcgt
    16681 tcacattcgg tccaacattg atactcaacg accagccaga atcatactct gttcatacgg
    16741 gcatctattg aaatgcccgt attttataaa taagcagtaa ccccaagagt aaacaacata
    16801 gtatttaaaa ccatcacacg aaaaattcag aagcattacg aaatatgaat ttacaaacaa
    16861 tagtggcata aatgttaacc atgttaattt acgtaaagtt ttacgttgca acattaaagc
    16921 ctcatttcaa tcatcatgat aaatataaaa ttaatatata tttatgccgt aaataccaat
    16981 atacttagca aactatgtga tctccatttc gattgattta gtgtttattg acgtatgtac
    17041 tggattatta acgataatat cgagttctgg cctgagcacc gaaaattaat atcggtacat
    17101 aacgccgatc ttaacgtcgt tctgacaacg ccagccagtc gatgtttatc acttctactt
    17161 gaagcttttc ctgatgtggt tacacaacaa gattttttca ccagagtctg ggaagaagaa
    17221 ggtatgcgtg tgcctactaa cacgttatat cagaacatat ccattatcag acgcggattt
    17281 cgcgctgttg gtgatactac ccactcgcta attgcaaccg tgccgagaga ggattcaa
//
LOCUS       DAESHI010000088        17139 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.089, whole genome shotgun sequence.
ACCESSION   DAESHI010000088 DAESHI010000000
VERSION     DAESHI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17139
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.089"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            190..2037
                     /gene="ldtD"
                     /locus_tag="KW011_003017"
     CDS             190..2037
                     /gene="ldtD"
                     /locus_tag="KW011_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBH7661300.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2218..2766
                     /locus_tag="KW011_003018"
     CDS             2218..2766
                     /locus_tag="KW011_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HBH7661301.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2793..3440
                     /gene="gloC"
                     /locus_tag="KW011_003019"
     CDS             2793..3440
                     /gene="gloC"
                     /locus_tag="KW011_003019"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HBH7661302.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3662..4852)
                     /gene="aspC"
                     /locus_tag="KW011_003020"
     CDS             complement(3662..4852)
                     /gene="aspC"
                     /locus_tag="KW011_003020"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HBH7661303.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5037..6125)
                     /gene="ompF"
                     /locus_tag="KW011_003021"
     CDS             complement(5037..6125)
                     /gene="ompF"
                     /locus_tag="KW011_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HBH7661304.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6726..8126)
                     /gene="asnS"
                     /locus_tag="KW011_003022"
     CDS             complement(6726..8126)
                     /gene="asnS"
                     /locus_tag="KW011_003022"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBH7661305.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8296..9498)
                     /gene="pncB"
                     /locus_tag="KW011_003023"
     CDS             complement(8296..9498)
                     /gene="pncB"
                     /locus_tag="KW011_003023"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBH7661306.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9764..12376
                     /gene="pepN"
                     /locus_tag="KW011_003024"
     CDS             9764..12376
                     /gene="pepN"
                     /locus_tag="KW011_003024"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HBH7661307.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12583..13350)
                     /gene="ssuB"
                     /locus_tag="KW011_003025"
     CDS             complement(12583..13350)
                     /gene="ssuB"
                     /locus_tag="KW011_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HBH7661308.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13347..14138)
                     /gene="ssuC"
                     /locus_tag="KW011_003026"
     CDS             complement(13347..14138)
                     /gene="ssuC"
                     /locus_tag="KW011_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HBH7661309.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(14150..15297)
                     /gene="ssuD"
                     /locus_tag="KW011_003027"
                     /pseudo
     CDS             complement(14150..15297)
                     /gene="ssuD"
                     /locus_tag="KW011_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            complement(15294..16253)
                     /gene="ssuA"
                     /locus_tag="KW011_003028"
     CDS             complement(15294..16253)
                     /gene="ssuA"
                     /locus_tag="KW011_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HBH7661310.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLIYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVSKKVDIRQRIWQPTHLEGKQL"
     gene            complement(16246..16821)
                     /gene="ssuE"
                     /locus_tag="KW011_003029"
     CDS             complement(16246..16821)
                     /gene="ssuE"
                     /locus_tag="KW011_003029"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HBH7661311.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQELLHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
BASE COUNT         4295 a         4421 c         4349 g         4074 t
ORIGIN      
        1 tacggcgcgc ttttctttaa acttctttac attaggttat gtaaaaacgt atcggcgttt
       61 atatactgaa gataagcctg atgagtaaca ggcttgctcg tcatactttc gtgagtattg
      121 gcgttgtaca ggcaagtcgt aaaataacag cctggctatt cagagtatga taaaaacagg
      181 gggcaaggga tgttgcttaa tatgatgtgt ggtcgtcggc tgtcggcaat cagtttgtgc
      241 ctggccgtaa cattcgctcc actgttcaat gcgcaggccg atgagcctga agtaatccct
      301 ggcgacagcc cggtggctgt cagtgaacag ggcgaggcac tgccgcaggc gcaagccacg
      361 gcaataatgg cggggatcca gccattgcct gaaggtgcgg cagaaaaagc ccgcacgcaa
      421 atcgaatctc aattacccgc aggttacaag ccggtttatc ttaaccagct tcaactgttg
      481 tatgccgcac gcgatatgca acccatgtgg gaaaaccgtg atgctgttaa agccttccag
      541 caacagctgg cagaggtggc gattgccgtt ttccagccgc agtttaataa atgggtagag
      601 ttactgaccg atcctggtgt taacgggatg gcacgcgacg tagtgctctc tgatgcgatg
      661 atgggctatc tccatttcat tgcaaatatt ccggtcaaag gcactcgctg gctatatagc
      721 agtaaacctt atgcacttgc aacgccgccg ctctcggtga ttaaccaatg gcagctggcg
      781 ctggataaag gtcaattgcc tacgtttgtt gcaggactgg caccgcagca tccgcaatat
      841 gcggcgatgc atgaatcgtt actggcctta ctcagtgaca ccaaaccgtg gccccaactg
      901 accggcaaag caacgttgcg cccagggcag tggagtaacg acgtaccggc gttgcgcgaa
      961 atattgcaac gcacaggcat gttggacggg gggccgaaaa ttactctacc tggcgatgac
     1021 acgccaactg acgcggtagt cagcccatcc gctgttactg ttgaaacagc agaaactaag
     1081 ccgatggaca agcaaacgac gtctcgtagt aaacctgcgc ctgccgttcg cgccgcctac
     1141 gataatgaac tggtggaagc cgttaaacgt tttcaggcat ggcaaggatt gggggcagat
     1201 ggtgctattg gcccggcaac acgtgactgg ttaaacgtaa cgcccgccca gcgtgctggc
     1261 gtgttggctc tcaacatcca gcgattgcgc ttgctgccaa cagagctttc taccgggatc
     1321 atggttaaca ttccggccta ttcgctggtc tactatcaga acggcaatca ggtgctggat
     1381 tcgcgagtca ttgtcggtcg ccccgatcgc aaaacgccga tgatgagcag tgcccttaac
     1441 aacgtagtgg taaacccgcc gtggaacgta cctccaactc tggcacgcaa agatattctg
     1501 ccaaaagtgc gcaacgatcc gggatatctc gaaagccatg gctatacggt gatgcgcggc
     1561 tggaacagca gagaagcgat tgacccatgg caggttgact ggtctacaat cacggcctcg
     1621 aatttaccgt tccgcttcca gcaggctcca ggcccacgga actcgctggg gcgctataaa
     1681 ttcaatatgc cgagttcaga ggccatttat ttgcatgaca cgccgaacca caatctgttc
     1741 aagcgtgata cacgcgcatt gagctcaggc tgtgtacgag tgaataaagc ttccgatctg
     1801 gcgaatatgc tgttgcagga tgcaggctgg aatgacaaac gtatttctga tgcgctgaag
     1861 cagggtgata cacgttacgt caatattcgg cagtcgattc cggtgaatct ctactacctg
     1921 acggcctttg ttggtgcaga tggtcgtacc cagtatcgta cagatattta caattatgat
     1981 ctgcctgcgc gatccagctc gcaaatcgta tcgaaagcgg aacaattaat caggtaaatg
     2041 aagtagttcg gggaaatcag ttgtcgtaat aattagcatg attgggggcg attctctgca
     2101 gcccccgtca ctgctggggt tgagtcatct tgacgtctgc tttacgggcg gttaaggtgc
     2161 ctcttgtgcg ccagaagtgc atataaacga taacattgac ctgtagactt gattatcatg
     2221 gacaaattcg acgctaatcg ccgcaaattg ctggcgcttg gtggcgttgc actcggtgcc
     2281 gccatcctgc cgacccctgc gtttgcaaca ctctctaccc cacgcccgcg cattttgaca
     2341 ctcaataatc ttcataccgg agagtcaatc aaagcggagt ttttcgatgg cagaggctat
     2401 attcaggaag aattggcaaa acttaaccat tttttccgcg attaccgcgc gaacaaaata
     2461 aagtccatcg acccaggatt attcgaccag ttgtatcgcc tgcaagggtt gttaggcacg
     2521 cgcaaaccgg tgcaactcat ttccggttat cgttctattg ataccaacaa tgaactacgc
     2581 gcccgcagcc gtggagtagc gaagaaaagc tatcacacta aaggccaggc gatggatttc
     2641 catattgaag gtatcgcgtt aagcaatatt cgcaaagccg cgttatctat gcgcgcaggt
     2701 ggtgtaggat attatccacg tagtaacttt gtgcatattg ataccgggcc agcacggcac
     2761 tggtagtaat tgcttaacga aacaggggca gtatgaacta tcgtattatt ccggtcaccg
     2821 cattctccca gaactgttca ttaatctggt gtgaacaaac ccgtctggcc gcactggtcg
     2881 atcctggcgg cgatgcggaa aaaatcaaac aggaagttga tgacagcggc ctgacactga
     2941 tgcagatcct gctgacgcat ggtcatctgg accacgttgg cgcagcggcg gaactggcgc
     3001 aacattacgg cgtgccggtt tttggcccgg aaaaagaaga tgagttctgg ctgcaaggct
     3061 tgcctgcgca aagtcgtatg tttggtctgg aagagtgcca gccgctgacg ccagatcgtt
     3121 ggctgaacga aggcgatacc atcagcatag ggaatgtgac tttacaggtg ttacattgcc
     3181 ctgggcatac gccgggtcat gtcgtgtttt ttgatgatcg ggcaaagctg ctgatttctg
     3241 gcgatgttat tttcaaaggc ggagtagggc gcagtgactt cccgcgtggc gatcataatc
     3301 aactgatttc ttcaatcaaa gataaattgc tgccactggg ggatgacgtg acatttattc
     3361 cgggtcacgg accattatcc acacttggtt atgaacgcct gcataatccc ttcctgcaag
     3421 acgaaatgcc cgtctggtaa ggcacataaa aaagcccgct tttaatgcgg gcctggattt
     3481 ctggcaaagt gcgctttgtt tatgccggat gcggcacgag cgccttatcc ggcctacaaa
     3541 atcgtgcaaa ttcaaaatat tgcaggggac gcgtaggcct gataagcgta gcgcatcagg
     3601 caatgttgcg tttgtcatca gtctcagccc gcttttcagc gggcttcatt gtttttaatg
     3661 cttacagcac tgccacaatc gcttcgcaca gcggagccat gttatctggt gtcatcccgg
     3721 ccacatttac gcgaccagaa gcaaccgcat atacgccaaa ctcttcgcgc agacgcagca
     3781 cttgttcttt tgtcaggcca ctgaaggaga acatgccgtt ctgtttgatg ataaagctga
     3841 agtcgcggtt tgcgcctttt tcctgcagcg tattgacgaa caactgacgc atacgctgaa
     3901 tacgctggcg catatcagtc agctcttgtt cccaaatcgc acgtaacgca tcgttgctca
     3961 ggatggtggc aacaacagaa gcgccgtgtg ctggtgggtt agagtagtta gcgcgaatcg
     4021 ccgctttcat ttggctgaat gcgcgatcaa cggtttcact gtcggcagca accagagtac
     4081 aagcgccaac acgctcgttg tacaggccaa agtttttaga gtaggaactg gcaacaatca
     4141 gctctttatg catagccgcg aaagcgcgca gtccttcagc atcttcttcc agaccacggg
     4201 caaaaccctg gtaagcgaag tcaaacagcg gtaaccagcc tttctcaacg gagagttgtg
     4261 ccagtgtctg ccattgttcc agcgtagggt cgataccggt tgggttatgg cagcagccat
     4321 ggaacagcac tacgtcgcca gcctgagctt cattcaggct gttaatcagt gcatcgaagt
     4381 caagagtgtg attttccgca tcataataag cgtattcacg aacttccaga cctgcagagt
     4441 taaagacgct cttatggttc ggccagcttg ggttgctcac ccacactcgc ttaacgctgg
     4501 tattttttgc caggaaatcg gcagctacgc gtagtgcgcc agtgcccccc ggagtctgtg
     4561 ccgtgcgagc acgtttgtca ttgatcaggg cgctaccttt accaaacagc agttcctgag
     4621 tgcagcgacc aaattcaggg atgccgtcaa tgccgaggta atttttggtg gtttcatttt
     4681 cgagcagata ctgttcagcc tttttcacgc tggtcagtac cggggttttg cccgtctcat
     4741 ctttatagac accaatcccg aggttaattt tgccgggacg ttcatcggca cgaaacagat
     4801 cggccaggcc cagaatcggg tcggcaggag cggcggtaat gttctcaaac atgacgaggt
     4861 tccattatgg ttacagaagg gaagtccgct atcagggtaa cgggagattt acaaaattcc
     4921 aactattact gatgaaaacg caggctgttt ttgcaagacg tgagattgct ctggaaggta
     4981 taaaaaaaac aggaccaaag tcctgttttt tcggcattta acaaagaggt gtgctattag
     5041 aactggtaaa cgatacccac agcaacggtg tcgtctgaac ctacgcccag tttgttgtca
     5101 gaatcgatct ggttgatgat gtagtcaaca taggtggaca tgtttttgtt gaagtagtag
     5161 gttgcgccca cttcaaagta gttcaccagc tcaacatcac cgataccttc tacgtctttc
     5221 gctttagatt tggtgtaagc gatggacgga cgcagaccga aatcgaactg gtattgcgca
     5281 actaacagaa cgtcttgcgt tttgttggcg aagccgctgg tgtttgtaaa tttattagtg
     5341 atcggcgtag cgttacgggt ttcaccgtag ttcgctgcca ggtagatgtt gttcgcgtcg
     5401 tacttcagac cagtagccca ctgttcagct tttttaccgt tgccaagagg ttgagcttct
     5461 tgcaggttgg tacggtcagc tgcaccataa gcaccaacga taccaaagcc ttcgtattcg
     5521 tagctgatag aaccgccaac accgtcgccg ttagaacggc gtgcagtgtc acgctcgttt
     5581 ttacccaggt actgaacagc gaagttcagg ccatcaacca gaccaaagaa gttggagtta
     5641 cgataggtag caacgccgcc aacacgacca acgaagaagt catcgctgta tgcagtatca
     5701 ccaccaaatt ctggcagcat atcggtgtaa cccagtgcat cataaaccac accgtagtta
     5761 cggccgtaat cgaaagaacc aacgtcagcg tatttaagac ccgcgaatgc cagacgcgtt
     5821 ttgttaccag tttgagcgtc agcgccttca gagttgttac cctggaagtt atattcccac
     5881 tgaccataac cggtcagatc ggaattgatt tgagtttccc ctttaaaacc aagacgggca
     5941 taggtcatgt cgccattgcc accgtaactg ttttcaccgt tacccttgga aaaataatgc
     6001 agaccaacag ctttaccgta cagatctact ttgttgccat ctttgttata gatttctgca
     6061 gcgtttgcag tacctgctac taacagagca gggacgatca ctgccagaat attgcgcttc
     6121 atcattattt attaccctca tggttttttt tatgacacct gccactgcgt caataagttc
     6181 tgtcaataaa aatttacgga actattgatg agagtttggt gtctttatgt gtctgcaggc
     6241 atctttccat tcaaactaac gtttcgctac cgtgaaagtg ctacaaagat aaagatttag
     6301 tttcaaaaag aaaaaatatg taaccaaaag taaaaattaa ggaactttgt gaacaccgtc
     6361 atatttccat agaaacgtga tgatatttac agcaattttt atctatttat atgatttact
     6421 tatatttaaa ttaactaaac ggaaattttg tttctgatgg aaactttatc gacctggcac
     6481 aaaatcctct ttcagacatc cagaatgcca aaaaatagta tgaaaattgt gctatcagct
     6541 aagaaaaaac taatccgcaa taagaatgcg ggttattttt tgtgcgtaaa tgttgcaatg
     6601 taatattccg taacaggatg atcgttatgg cggggttgca aatagattgc ttgacttaac
     6661 aaacagataa cttgacagaa aggataaaaa agagccagca ggcgctggct ctttgaggaa
     6721 gaaaatcaga agctggcgtt gcgcggagta cgtgggaacg gaatcacatc acgcacgttt
     6781 tgcacgccag ttacgtaagc aatcaggcgt tcaaaaccaa gaccgaaacc tgaatgcgga
     6841 acagtaccgt agcgacgcag atcgcgatac caccagtaat cttctttatt caggcccatt
     6901 tccagcatac gctcgtccag aacgtccaga cgttcttcac gctgggagcc accaatgatc
     6961 tcaccgatgc ccggagccag aacgtccata gccgcaacgg ttttaccgtc ttcgttgagg
     7021 cgcatataga acgctttaat atctttcgga tagtttttaa ccactaccgg tgctttaaag
     7081 tgttcttccg ccagataacg ctcatgctca gaagagagat cgacgcccca gtaaaccggg
     7141 ttttcaaact tcctgccgca gttttcgaga atggtcactg cgtcggtgta atccacctgc
     7201 gcgaaatcgg cctcaatgaa gcgttccaga cgtgaaacgg catctttatc tacgcgttca
     7261 gcgaagaatt tcatgtcgtc tgcgcgttct tcgagaaccg ctttgaagac atatttcagc
     7321 atggcttcag ccagacccgc gatatcgttc aggttagcaa acgccacttc cggctccagc
     7381 atccagaatt ccgccaggtg acggctggtg ttggagtttt cagcacggaa agtcgggccg
     7441 aaggtataaa ttttggataa tgcgcaagcg taggtttcgc cgttcaactg gccagatacg
     7501 gtcaggaaag actctttacc aaagaagtct ttgtcgaaat ccactttgcc ctgatcgtta
     7561 cgcggcaggt tttccagatc cagcgtagaa acgcggaaca tttcgcctgc accttcggta
     7621 tcagatgcgg taatcagtgg cgttgaaacc cagaagaatc cctgctcgtt aaagaagcga
     7681 tgcagcgcct gcgccagcgt atggcgaacg cgcgcgacgg caccaatcag gtttgtgcgc
     7741 ggacgcaggt gagcgacttc acgcagatac tcaatgctgt ggcgttttgc cgccatcggg
     7801 taagtgtctg gatcttcaac ccaaccagca acttcaacct tgctggcctg aatttcaaat
     7861 tgttgcccct ggcccggcga tgccacgact ttacccgtca caatgaccga gcagccggtg
     7921 gtcagacgca ggacgtcttc attgtaattg ggcagagaat tattgatgac agcctgtaca
     7981 ggatcaaagc aggaaccgtc ataaacggcg aggaaggaga tgccagcttt tgaatctcgg
     8041 cgggtacgta cccatccgcg tacggtgact tcgctgtcaa cggctacgcg gccctggagt
     8101 acgtcggcta caggcacaac gctcataata ttctctctgt taatagtcgg aaaaaataaa
     8161 cacttgtcca cccgtaaatg ggggggatac ctatgttacc tggcatctgc aatcagacaa
     8221 gcagaattcg caaatgcagc gaaagatttc ggaattaaag gtaaaaaaag ggagccgatt
     8281 agctccctga tgatattaac tggctttttt aatatgcgga aggtcgaacg ctttgcgcag
     8341 cgcccgaaca aacgctttat catggcagat agttttgcca gggctgtcag aaagtttcgc
     8401 caccggttta ccgttacact ctaccaactt aatcacaata ttcaggggtt ttacctgggg
     8461 gatatcgcag gtcaggcgag tcccaatacc aaaacttaat tgcacgcggg aagagaagtg
     8521 gcggtatagc tcaaccgctt tgcgtaaatc cagattgtca gagaaaacca gcgttttact
     8581 ctgtggatca attcccagct tttcataatg tgcaatggct ttttcacccc attcaaccgg
     8641 gtcgccagag tcatgacgca ggccctgata ccgactagcg aactcgacac cgaaatcacg
     8701 caggaaagca tccatagtga tgcagtcggt taatgcaatg ccaagttggt cgggatactc
     8761 ttccagccag gcagcaagtg cagctcgctg gctgttggct agatccgggc tgatttgctg
     8821 atgtgcctgg aaccattcgt gtgcctgtgt tcccatcggc gtgagggaaa gccgacgcgc
     8881 cagatcgtag ttgctggtgc ccacgaacca ggattcctgt tgcagacgct taacgatggt
     8941 ttcttgtact tcgcgagaaa aacggcgacg ggtgccaaaa tccatcagat ggaagcgcga
     9001 catatcaaga ccggcggtta acgccgagaa gtcgactaat ttgctttcca gcgtgtcgag
     9061 ggcttgcgca acgtcggcct gcggtgagcg atagcgatgt accatttcac tgataaccgc
     9121 cagcaaagga acttcccaga ggatgacttc acgccacgga ccgcttaagc gaatatccag
     9181 cttgccatta tcgttggaca cggtgacttg ttccgggtta aagcggaact cgcgtaacca
     9241 gttaagatag tcggccttaa agaaaggcag ggcagagagc cactgatatt catcatcctg
     9301 caggcgcagg tgctgcatcg cctgaatctg ttcacgaata gcatcggcat aaatacccag
     9361 cagatcgtca cctcggcaac gaaactccgc cgcgacatgc acatcgtaat agtgatgaaa
     9421 cacggcttgc tgcatatgca acttataagc atctgtatcc agcaacgagt gcagaacagg
     9481 agaagcgaat tgtgtcatag gtgcgcagta gcgtcctcgt acaggagcgt ttagtacaat
     9541 aaacatcttc aggaactgct ggagtatacc ttgtttcgca atttattgaa ccccgatcac
     9601 accatatgcc acctttctgg tcgatggcat tacgcggtgt atgttataaa aatgtagcaa
     9661 taaaggcgtt tgtacctgaa aagatgaaga ttctgcatag cgcgatttac gcaacaggaa
     9721 tagactgaac accagactct ataaaagatg ctaaaggtta tttatgactc aacagccaca
     9781 agccaaatac cgtcacgatt atcgtgcgcc ggattaccag attactgata ttgacttgac
     9841 ctttgacctc gacgcgcaaa agacggtcgt taccgcggtc agccaggctg tccgtcatgg
     9901 tgcatcagat gctccacttc gtctcaacgg cgaagacctc aaactggttt ctgttcatat
     9961 taatgatgag ccgtggaccg cctggaaaga agaagagggc gcactggtca tcagtaattt
    10021 gccggagcat tttacgctta agatcgttaa tgaaataagc ccggcggcga ataccgcgct
    10081 ggaagggctt tatcagtcag gcgatgcgct ttgcacccag tgtgaagccg aaggtttccg
    10141 ccatattacg tattatctcg accgcccgga cgtgctggcg cgttttacca ccaaaattat
    10201 tgccgataaa accaaatatc ccttcctgct ttccaacggt aaccgcgttg cgcaaggtga
    10261 actggaaaac ggacgccatt gggtacagtg gcaggacccg ttcccgaaac cgtgctacct
    10321 gtttgcgctg gtggcaggcg actttgatgt actgcgcgac acctttacca cgcgttctgg
    10381 tcgcgaagtg gcgctggagc tgtacgtcga tcgcggcaac cttgatcgcg cgccgtgggc
    10441 gatgacctcg ctgaaaaact caatgaaatg ggatgaagaa cgcttcggcc tggagtatga
    10501 cctcgacatc tatatgatcg tcgcggtgga tttcttcaat atgggcgcaa tggagaataa
    10561 gggtctgaat atctttaact ccaaatatgt gctggcccgc accgacaccg ccaccgacaa
    10621 agattacctc gatattgaac gcgttatcgg ccatgaatat ttccataact ggaccggtaa
    10681 ccgagtgacc tgccgcgact ggttccagct cagcctgaaa gaaggtttaa ccgtcttccg
    10741 cgatcaggaa ttcagctctg accttggttc ccgcgcagtt aaccgcatca ataatgtgcg
    10801 caccatgcgc ggattgcagt ttgcggaaga cgccagcccg atggcgcacc caatccgccc
    10861 ggacatggtc attgagatga acaacttcta caccctgacc gtttacgaga agggcgcgga
    10921 agtgatccgt atgatccaca ccctgctggg cgaagaaaac ttccagaaag ggatgcaact
    10981 ctatttcgag cgtcatgatg gtagcgcagc gacctgtgac gactttgtgc aggcgatgga
    11041 agatgcgtcg aatgttgatc tctcccattt ccgccgttgg tacagccagt ccggtacgcc
    11101 gattgtgacc gtcaaagacg actacaatcc ggaaactgag cagtacaccc tgaccatcag
    11161 ccagcgcacg ccagccacgc cggatcaggc agaaaaacag ccgctgcata ttccgtttgc
    11221 catcgaattg tatgacaacg aaggcaaagt gatcccgttg cagaaaggcg gtcatccggt
    11281 gaattccgtg ctgaacgtca cccaggcgga acagaccttt gtttttgata atgtctactt
    11341 ccagccggtg cctgcgctgc tgtgcgaatt ctctgcgcca gtgaaactgg aatataagtg
    11401 gagtgatcag caactgacct tcctgatgcg tcatgcgcgt aatgatttct cccgttggga
    11461 tgcggcgcaa agtttgctgg caacctacat caagctgaac gtcgcccgtc atcagcaagg
    11521 gcagccgctg tctctgccgg tacatgtggc tgacgctttc cgcgcggtgc tgctcgatga
    11581 gaagattgat ccggcgctgg cggcagaaat cctgacgctg ccttctgtca atgaaatggc
    11641 tgaactgttc gatatcatcg acccgattgc tattgccgaa gtacgcgaag cactcactcg
    11701 tactctggcg actgaactgg cggatgagct gctggctatt tacaacgcga attaccagag
    11761 cgagtaccgt gttgagcatg aagatattgc aaaacgcact ctgcgaaatg cctgcctgcg
    11821 cttccttgct tttggtgaaa cgcatctggc tgacgtgctg gtgagcaagc aataccatga
    11881 agcaaacaat atgaccgatg cgctggcagc gctttctgcg gcggttgctg cacagctgcc
    11941 ttgccgtgac gcgctgatgc aggagtacga cgataagtgg catcaggacg gtctggtgat
    12001 ggataaatgg tttatcctgc aagccaccag cccggcggcg aatgtgctgg agacggtgcg
    12061 tggtctgttg cagcatcgct catttaccat gagcaacccg aaccgtattc gttcgttgat
    12121 tggtgcgttt gcgggcagca acccggcagc gttccatgcc gaagatggca gcggttacca
    12181 gttcctggtg gaaatgctta ccgacctcaa cagccgtaac ccgcaggtag cttcacgtct
    12241 gattgaaccg ctgattcgcc tgaaacgtta tgatgccaaa cgtcaggaga aaatgcgcgc
    12301 ggcgctggaa cagttgaaag ggctggaaaa tctctctggc gatctgtacg agaagatcac
    12361 caaagcactg gcttgatact tttgagccaa aatgcctgat gcgacgcttg acgcgtctta
    12421 tcaggcctac aactccgttg aaattgctgg ttttgtaggc tggataaggc gttcacgccg
    12481 catccggcag ccgtgcccaa ctgcctgatg cgatgcttga cgtgtcttat caggcctaca
    12541 agtccgttga aaatgctggt tttgtgcgcc acatccggca tattaaccat gacgccgaat
    12601 cagttgttca gaatgcccac gccgcattac ccgttgcaat acttccgctt ccagctccgc
    12661 taatcgcacc gaccctaatc gacgcggacg ggggatatcc actgtcaaat ccagaccaat
    12721 ttttccctct tcaattaaca acacccggtc agccattgct accgcttcgc tcacatcatg
    12781 cgtcaccaac agtacggtaa agccgtgctg ctgccaaagt gacacaatca aatcctgcat
    12841 ctcgagtcgc gttaaggcgt ccagtgcccc gagcggttca tcaagcaaca ataaacccgg
    12901 tcgatgaatc aacgctcttg ccagcgccac acgctgtttc tgcccgccag aaagtgcggc
    12961 aggccattcc ccggcgcgat tctccagtcc tactgcagcc aacgcccgac gcgcggcatc
    13021 gcgccactgg cctttaaggc ctaacccaac gttatcaatc accgatttcc acggcagcag
    13081 acgcgcatcc tgaaacatca ttcgcgtgtc ttcctgaatc tcagccagcg gtgtggtgcc
    13141 cgctaacaca tcgcctgcgg ttggcgtttc cagacctgcc agcaggcgca gcagggtact
    13201 tttgccacca ccgctgcggc ccaccaccgc cacaaactga cctgccggaa tatgtaaatc
    13261 cagttggttc aggacgatat tttccgcgta atgtttgctt actgcattga gcaacaatgg
    13321 tgtgccctgg ttcagacgag cagtattcat accgtggcct ccttcaaatg ataagccggg
    13381 ttccagcgca accagaggcg ctctaacagc tgcgcgctga cgtcagcaag tttgccgagc
    13441 agggcgtaaa gaataatagc gactaccacc acgtccgttt gcaaaaactc ccgcgcattc
    13501 atcgccagat aaccaatgcc tgaattggca gaaatggttt cggcaacaat cagcgtcagc
    13561 cacatcaggc ccaacgcaaa acgcacgccg accataattg agggcagggc accaggcagg
    13621 atcacatgga taaacagcgg tatgccggat aatccatagc tacgcgccat ttccaccaga
    13681 ccgcgatcga tattacggat gccatgccag gtgttgatat aaatggggaa tagcgtgccg
    13741 agcgccacca gaaagatttt tgcggattca tcaatgccaa accacaaaat caccagcggg
    13801 atcagcgcca gatgcggtac gttgcgcaac atctgaattg aggtatccag cagccgttct
    13861 ccccagcgtg acagcccgct aatcagtccc agaatcaagc ccagcgatcc gccaattgaa
    13921 aagccaatca gcgcccgcca ggaactgatc gccagatgct gccacaattc gccgctggcg
    13981 gagagcgtcc agaacgccgt caccactcct tccggtgaag gcaaaatacg cgtcgacagc
    14041 cagccaaccg aggaggccag ttgccacacc gccacgatgc ccaccggtaa aaaccagggg
    14101 gcaacgcgca ataaccactt cttcactggc gttgccatca tcgtgttcct tagctttgcg
    14161 cgactttacg ggggataaaa tcattcgcca ccgcttcgcc ttgcggattc agcggctgcg
    14221 gctggggaat ttccggaatg gcgacatcca gatgtgggaa cagcaactcg ccaacccgat
    14281 acgcttcttc cagatgcggg tagcccgaaa gcacaaagct gtcgatgcca agcgcggcgt
    14341 attcgttgat tcgcgcggcg accgtaggac catcgcccac cagcgccgtt ccggcaccgc
    14401 cgcgcactaa gccaacgccc gcccataaat tggggctgat ctccagattg tcgcgcttgc
    14461 cgttatgtag cgccgccatt cgctgttgtc ctacggaatc cgtccgggcg aatgcggcct
    14521 gtgctttggc gatagtttca tcatcaagat gcgagattaa ccgctcggcg gcctgccacg
    14581 cttcgtcgtt agtttcacga acaatcacat gcagacgaat accgaaacga attttgcgtc
    14641 catgcgcggc agctttcgcc cgcacttgtt cgatcttctc ttttaccagc tctggcggtt
    14701 cgccccaggt gaggtagaga tcaacttgtt ctgccgccag ctcctgggcg acatctgacg
    14761 atccgccaaa gtaaagcgga ggatacggct gttgaatcgg cgggaagagc agttttgctc
    14821 cccgcacatg aatatgttta ccgttgaaat cgacgctttc ccccagcaat aagcgccccg
    14881 ccagacctgg gtaaattccg ccgaggcttc gtagcgctcg ctatgatcaa ggaacacgcc
    14941 gtcgcctgcc aactcttgtg gatcgctgcc tgtgaccaga ttaaacaacg cacgaccatt
    15001 tgagagacgg tcaagcgtgg cggcctggcg agcggcaacg gtaggtgagg ttacgctggg
    15061 acgcagggcg acaagaaact tcagccgctg cgtcaccggg atcatcgacg ccgccaccag
    15121 ccacgcatct tcacacgatc gccccgttgg gatcagcaca ccggtatagc ccagacgatc
    15181 cgccgcttgc gcaatttgtt gcagatagcc gtgatcaacc gggcgtgaac cttcttccgt
    15241 tccaagataa tgcccgtcac catgggtcgg taaaaaccag aacatattca gactcataat
    15301 tgttttcctt ccaggtgagt gggctgccag atgcgctggc gaatatcaac ttttttcgac
    15361 accagacggt tttcataaaa cagatctgcc gtttgctgct gtaaggcggc aacttcagcg
    15421 ctcaccggtt tgatggtggt gggaggacga tgatccagat acgaggcaat caccggtgcc
    15481 ggtaagccca tcgtttttgc cagtaaagcg atgctttgct cgcgctggct gcgggttaac
    15541 gcatcggcct cactaaaggt tgccagtacg ccctgaataa aagcgccgtt tttttctgca
    15601 taggggcgag ctgccagata aaacgatcca gtttgattga gatcggtgcc gtctttcagc
    15661 acccgcacgc cgccctgtaa taacgcagcg gagtagtagg gatcccagat agcccaggcg
    15721 tcaacgttac cttgctggaa cgcggcgcgg gcatcagctg gcgtcagata agtgggctgg
    15781 atgtcagtaa acttaagtcc ggcctgacgc agtgcacgca gtaaaaggtt gtgtgaactg
    15841 gaacctttct gaaaggcaac tttgtgacct ttaagatcgg ctacggtttt gatcgggctg
    15901 ttttctgcca ccagaatcac ttcggctttg ggcttcggtg gctcgacgcc cacatatatc
    15961 aaatcagccc cggcagcctg ggcaaagatt ggcggaatat ccccggtact gccgagatca
    16021 atactgccaa cgtttaacgc ttccaacatt tgcggacccg cggggaactc cacccaggag
    16081 atttttgatt gcggatagcg tttttccagt aactggtggc tttttgccaa taccatacca
    16141 atactgcctt tctgatagcc tatacgtaac gcttcaggcg aggattctgc agcaaccgca
    16201 aaagtagaga cgctaagcaa tcccgccagc gccagtttaa tgatgttacg catggacatt
    16261 acctcgcaga gacagaaggt caggaacctg aacatcgcgg cggtgcaatg cctgccagaa
    16321 agtttctagc gcggtatcaa gacgggtttg cagatttggc gtgaactggg gtttgtgatg
    16381 gtaatcaata acttgtgagt catcggcaaa cacgccgtgc aggagctcct gggctttcat
    16441 cgcacttaaa actggcttaa gggcataatc gaccgccagc agatgagcca ccgtaccgcc
    16501 cgtcgccagc ggtagcacca ctttgccttg caaagcgcgc tctggcagca ggtcgagcag
    16561 ggttttcagc gcgccggaat aggcggcttt atacacaggt gtagcgacta tcagtccatc
    16621 agcctgttgc agctgttcgg tgaaggtctt gagtgccgga ctatcgaaac gagcgtaaag
    16681 cagatcttcc ggggcgaagt tttgcagatt ccagtgataa acctctacat ccaggccatt
    16741 tagtttttcc cgcgcatatt ccagcaagga gctggagcga gaaggaaagc gaggactacc
    16801 cgccagggtg atgacacgca tactctctcc ttataaccaa ttgttctttt tttgttaaca
    16861 ttgataacaa ttcggtcagt ctgtcggaga gacaagaaaa tgccaaatat aaattttgtg
    16921 tatctttttc tgaaaataaa ctaaagaaag ggctttatat atcacgcata tttatttgtt
    16981 ggatagtcat tagatgttga atcaatgaat tactcgttca gttaatattt aaatattcat
    17041 gaaatctata aattaaagat ttgtcactta ttggatttag tatgcttgat ttaaatggcg
    17101 gtcaattatt gttattatta agtctaatgt catttaaag
//
LOCUS       DAESHI010000089        16400 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.090, whole genome shotgun sequence.
ACCESSION   DAESHI010000089 DAESHI010000000
VERSION     DAESHI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16400
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.090"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            78..2411
                     /gene="bisC"
                     /locus_tag="KW011_003030"
     CDS             78..2411
                     /gene="bisC"
                     /locus_tag="KW011_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HBH7661312.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2380..2820)
                     /locus_tag="KW011_003031"
     CDS             complement(2380..2820)
                     /locus_tag="KW011_003031"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBH7661313.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2817..3380)
                     /gene="tag"
                     /locus_tag="KW011_003032"
     CDS             complement(2817..3380)
                     /gene="tag"
                     /locus_tag="KW011_003032"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBH7661314.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3538..4236
                     /locus_tag="KW011_003033"
     CDS             3538..4236
                     /locus_tag="KW011_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBH7661315.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4465..5673
                     /locus_tag="KW011_003034"
     CDS             4465..5673
                     /locus_tag="KW011_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH7661316.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5997..7688
                     /gene="eptB"
                     /locus_tag="KW011_003035"
     CDS             5997..7688
                     /gene="eptB"
                     /locus_tag="KW011_003035"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HBH7661317.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7780..7856
                     /locus_tag="KW011_003036"
     tRNA            7780..7856
                     /locus_tag="KW011_003036"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7814..7816,aa:Pro,seq:cgg)
     gene            7891..8024
                     /locus_tag="KW011_003037"
     ncRNA           7891..8024
                     /ncRNA_class="other"
                     /locus_tag="KW011_003037"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8769..10376
                     /gene="dppA"
                     /locus_tag="KW011_003038"
     CDS             8769..10376
                     /gene="dppA"
                     /locus_tag="KW011_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HBH7661318.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10684..11703
                     /gene="dppB"
                     /locus_tag="KW011_003039"
     CDS             10684..11703
                     /gene="dppB"
                     /locus_tag="KW011_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HBH7661319.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11713..12615
                     /gene="dppC"
                     /locus_tag="KW011_003040"
     CDS             11713..12615
                     /gene="dppC"
                     /locus_tag="KW011_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HBH7661320.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12626..13609
                     /gene="dppD"
                     /locus_tag="KW011_003041"
     CDS             12626..13609
                     /gene="dppD"
                     /locus_tag="KW011_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBH7661321.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13606..14610
                     /gene="dppF"
                     /locus_tag="KW011_003042"
     CDS             13606..14610
                     /gene="dppF"
                     /locus_tag="KW011_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HBH7661322.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14640..15911)
                     /locus_tag="KW011_003043"
     CDS             complement(14640..15911)
                     /locus_tag="KW011_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBH7661323.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         3900 a         4094 c         4409 g         3997 t
ORIGIN      
        1 ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat acatctaacc
       61 tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg actgccgccc
      121 actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg cgtggcgcgt
      181 tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt cacagcaata
      241 cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa aacccgcaag
      301 gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg gatcttattc
      361 accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt gctggttcct
      421 acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa cgctatatgg
      481 cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg gcacaggcga
      541 tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg
      601 tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa
      661 ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg cgtgacagtg
      721 ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata
      781 aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc
      841 atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc accacaggtt
      901 atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa accgccgaat
      961 gggcagcaga gatttgtggt gttggcgcag cgaaaatccg ccagctggcg gctattttcc
     1021 accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag tttggtgagc
     1081 aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc acacccggcg
     1141 gcggttttgg tctttcttac cattttgcca atggtggtaa ccccacgcgg cgttctgcgg
     1201 tgctctcttc catgcagggc agcttgccgg gtggctgcga tgcggtggat aaaatccctg
     1261 ttgcccgcat tgttgaagca ctggaaaacc ctggtggcgc atatcaacac aacggtatga
     1321 accgacattt cccggatatt cgttttatct ggtgggcggg cggtgccaac tttactcatc
     1381 atcaggatac caatcgtctg atccgtgcct ggcaaaaacc ggagctggtg gtgatctctg
     1441 aatgcttctg gacggcggcg gcgaaacacg cggatatcgt tctgcctgcg actacctctt
     1501 ttgagcgtaa tgatctcacc atgaccggtg attacagtaa tcagcatctg gtgccgatga
     1561 agcaagtggt gccgccacgc tatgaagcgc gtaatgattt tgatgttttt gccgagttga
     1621 gtgaacgttg ggagaagggc ggctatgcgc ggtttacgga aggaaaaagt cagctgcaat
     1681 ggctggaaac gttttataac gttgcccggc agcgcggggc aagccagcag gttgaattgc
     1741 cgccatttgc tgagttctgg caagccaacc agttaattga gatgccggaa aacccggaca
     1801 gcgagcggtt tattcgcttc gctgattttt gccgcgatcc gctggcgcat ccgttaaaaa
     1861 ccgccagcgg caagattgaa atcttctcac agcgtattgc cgattacggt tacccggatt
     1921 gccctgggca tccaatgtgg ctggagccgg acgaatggca gggcaatgcc gaaccagaac
     1981 agttgcaggt actttctgcc catccggcgc accgcctgca cagccagctg aattacagtt
     2041 ctctgcgcga attgtacgcg gtagcaaacc gtgagcctgt caccattcat cctgacgatg
     2101 ctcaggcgcg cggcataacc gaaggggata tggtgcgggt gtggaactcc cgtgggcaga
     2161 tccttgccgg agcggtcatt agcgagggaa ttaaacctgg cgtgatttgc attcacgaag
     2221 gggcatggcc ggatctggat ttaaccgctg acggtatttg taaaaacggc gcagtgaacg
     2281 tgctgaccaa agatctcccc agctcgcggc tggggaatgg ctgtgcgggt aatacggcgc
     2341 tggcatggct ggaaaaatac aacggtccgg aactgacact tacagcgttt gaaccaccgg
     2401 ccagctcata atccaggtgg gtagttgggt ttcatcctgc catgcgcaat cgacaatgtg
     2461 aaaaccctgc gcacggtaaa aatctatcgc cggttgattt ttttgataaa cctccagcat
     2521 cagatgggga tagcgctgct gcacatactg catcagcgcc ttaccaatac cgcgtctgac
     2581 ggccttcggt gcgacaaaca ttgctgccag aaatcggcct tccataatgc tgacaaaacc
     2641 gagaagctta ccgtcctctt cccagaccca gttttgcgcg ttggcaagat aggcatcccg
     2701 caccagcgga atgcagtcac gccagtaatt cgcttttata aagggatgcc cccaggttgt
     2761 actttccagc cacagttcga ggatcgcggg gagttctgaa cgttgcgctt cccgaatcat
     2821 ggtttatttc ccggatagca acagcagcca accacatgat cattcaccag cccacatgcc
     2881 tgcataaagg agtaacagat tgtggtgccg acaaacttaa aaccacgttt tttcagtgct
     2941 ttagataggg cgtcggaggc ggacgtagat gtgggaattt cgctcaacgt tgtggcttgt
     3001 gtcacctgtg gctgatgatt tacaaacgac cagacaaagt cggcaaacgg ttcgccgttc
     3061 tgttccattt gcaggtacgc ccgcgcatta ccaataattg cctgaatttt ccctcgatgg
     3121 cggataatcc cggcgtcctg taccagtctt tcgacatcct cttcctgcat tgctgcgacc
     3181 ttcaccggat cgaactgatg aaagcaggcg cgatagtttt cgcgtttttt gaggacggtg
     3241 atccacgata acccagcctg ctgcccttca aggcagatca tttcgaacag ttttttactg
     3301 tcagtttcag gcacgcccca ttcattatca tggtaggcaa tataaagcgg gtcctgactc
     3361 acccagccgc aacgttccat actttccctc gctatgtacc cgatcgtaaa aatttcagcc
     3421 gcttttcttg acgcatcggt gaaaaagtca atatttaata cagggctact atgcccgata
     3481 tccttcatac aatgacaata atattgccga attcggctct tccctggagt cgtgttaatg
     3541 atcataaaaa aaagcggtgg tcgctggcag ctaagcctgc tggcgagcgt ggtaatcagt
     3601 gccttttttc tcaacacagc ttacgcctgg caacaagaat atatcgttga tacgcaaccc
     3661 ggacattcca cagagcgtta cacctgggat agtgatcatc aacctaatta caacgatatt
     3721 ttgtcgcaac gtattcaaag tagccaaagg gcgctgggac tggaagtcaa tctggcggaa
     3781 gaaactcctg tggatgtgac cagcagtatg agtatgggct ggaattttcc tttatatgaa
     3841 caggttacaa ccggcccggt cgcggcatta cattacgatg gcacaaccac ctcgatgtat
     3901 aacgagtttg gcgacagtac taccacgcag accgatccgt tatggcatgc cagcgtgagt
     3961 accttaggct ggcgtgttga ctcccggctt ggcgatctcc gaccctgggc gcaaatcagc
     4021 tataaccagc aatttggcga gaatatctgg aaggcgcaat caggcctgag ccggatgacg
     4081 gcgacaaacc agaacggcaa ctggctggat gtcaccgtag gcgctgatat gttgctcaat
     4141 caaaatattg ccgcctatgc cgcgctaact caggcagaaa ataccactaa taatagcgac
     4201 tatctgtata cgatgggggt tagcgccaga ttttaacgta acagtcacaa ttgaaaccat
     4261 taaataacaa taattgtggc gatagtgggt gctaacttac caaataataa atttggtgaa
     4321 taattgtcgc gtcattcatt cctgaactaa ggcatttcat tccgttctga tggcatttca
     4381 tgccgttttt ccccaggcat aaagtgcact tcgttatggt tgtcggcaga gatttttcct
     4441 ttttattact gcaggaatac tgccatgaca ccttcaaatt atcagcgtac ccgctggctg
     4501 acactcatcg gtactatcat tacccagttt gcgctggggt cggtttatac ctggagcctg
     4561 tttaatggcg cgctttccgc caagctggat gcgccggtaa gccaggtcgc tttctctttc
     4621 ggcttgttaa gtctggggct ggcaatttcg tcttctgttg cgggcaaatt acaggaacgt
     4681 tttggcgtta aacgcgtcac catggcttcc ggcattttgc tgggattagg cttcttcctg
     4741 accgcgcatt ccaacaacct gatgatgctg tggttaagcg ccggtgtgct ggtcggtctg
     4801 gcggatggcg cgggttacct gctgacgctc tctaactgcg tgaagtggtt cccggagcgt
     4861 aaaggtctga tctccgcgtt cgctatcggt tcttatggtc tgggtagcct gggtttcaaa
     4921 tttatcgaca cgcagctgct cgaaacggtg ggtctggaaa aaacatttgt gatttgggga
     4981 gcgattgcgc tggtgatgat tgttttcggc gcaacgttaa tgaaagacgc accaaaacag
     5041 gaagtgaaaa ccagcaatgg tgtggtggag aaagattaca cgctggcaga gtcgatgcgt
     5101 aaaccgcagt actggatgtt agcggtaatg ttcctgactg cctgcatgag cggcctgtac
     5161 gtgattgggg tagcgaaaga tatcgcccaa agtctggcac accttgatgt ggtttccgca
     5221 gccaatgcag taacggttat ttccatcgct aacctttcag gtcgtctggt gctgggcatt
     5281 ctgtctgaca aaattgcccg tatccgtgtt atcaccattg gtcaggtgat ttcgctggtg
     5341 ggtatggcgg ccctgctgtt tgcaccatta aatgcagtga cgttctttgc agcgattgcc
     5401 tgcgtggcat ttaactttgg cggcactatt accgtctttc catcactggt cagtgagttc
     5461 ttcggcctca ataacctggc gaaaaactac ggtgtgattt acctcggttt cggtatcggt
     5521 agcatttttg gttcgattat cgcctcactg tttggcggtt tctatgtgac cttctacgtg
     5581 attttcgccc tgctgattct gtcattggcg ctttctacga cgattcgtca gccagagcag
     5641 aaaatgttgc gtgaggcgca tggctccctt taatgctcag ccgcgagcta ctgggcttca
     5701 gcttccgtgt gcatcaaacg catcaacgaa atgtataaac aatgactcgt aaaatttggg
     5761 ccagttaact ggcccttttt actgcttttt gtaacgctaa agtctctttt caaacttgca
     5821 tttttgtaaa tttgtgcttc atgcacactc tttccccaca ctttttcgct ttgctgtggt
     5881 ctacttattc gcgcgtgtag attttactta tctgactacc tccgcacttt tcccctgccg
     5941 ggcctgaaaa gccgttaagc agggtgttat cacctgtttg tccagggttt gtttgcatga
     6001 gatacatcaa atcgattaca cagcagaagc tgagcttttt gcttgcaatc tatattggcc
     6061 tttttatgaa tggcgcggtt ttttatcgcc gcttcggcag ctatgcgcac gattttaccg
     6121 tctggaaagg cgtttctgct gttgttgaac tggccgccac cgtactggtg accttctttt
     6181 tactacgtct tctatcgctg tttggccgcc gcagctggcg tattctggcg tcgctggtgg
     6241 tgctcttttc cgcaggtgcc agctattaca tgaccttcct taatgtggtc attggttatg
     6301 gcatcatcgc ttccgtcatg accaccgata tcgacctgtc aaaagaagtt gttggtctga
     6361 actttattct ctggttaatc gccgttagtg cattgcctct tatccttatc tggaataacc
     6421 gctgtcgcta caccttgctc cgacaactgc gaaccccagg gcagcgtatt cgcagcctgg
     6481 cggtcgtcgt actggcgggt attatggttt gggcaccgat tcgtttgctg gatatccagc
     6541 agaagaaagt ggagagggcg accggcgttg atttgccgag ttatggcggt gtcgtagcga
     6601 actcttatct gccatcaaac tggctttctg cgttggggct gtatgcctgg gcgcgggtcg
     6661 atgaatcttc cgataataat tcattgctta atccggcgaa gaagtttaca taccaggctc
     6721 cacaaaacgt tgatgatact tatgtcgtgt ttatcatcgg tgaaaccacg cgttgggacc
     6781 atatgggtat tttcggctat gagcgtaata ccacgccgaa actggcccag gagaaaaatc
     6841 tggcggcgtt ccgtggttac tcctgtgata ccgcaaccaa actctcactg cgttgcatgt
     6901 ttgtacgtca ggggggcgcg gaagataatc cgcagcgcac attaaaagaa cagaacattt
     6961 tcgcggttct gaagcagtta ggattcagtt ctgacctcta cgctatgcag agcgaaatgt
     7021 ggttctacag caacacgatg gcggacaaca ttgcttatcg tgagcagatt ggtgcggagc
     7081 cacgtaatcg tggcaagccg gtagatgata tgttgctggt agacgaaatg cagcaatcgc
     7141 tagggcgcaa cccggatggt aagcatctga tcattctgca taccaaaggt tcgcatttta
     7201 actacaccca gcgttatccg cgtagcttcg cgcagtggaa gccggaatgt attggtgttg
     7261 atagcggctg taccaaagcg cagatgatca actcctatga caactcggtg acctatgtgg
     7321 atcactttat ctccagcgtg attgatcagg ttcgcgataa gaaagcgatt gtgttctacg
     7381 cagctgacca cggtgagtca attaatgaac gcgagcacct gcacggcacg ccgcgtgaac
     7441 tggcaccgcc ggagcagttc cgcgtaccga tgatggtctg gatgtcagat aaatatctgg
     7501 aaaatccggc caatgcgcag gcgtttgcgc agctgaaaaa agaagccgac atgaaagtgc
     7561 cacgccgtca cgtagagctg tacgacacca tcatgggttg tcttggctat acttcaccgg
     7621 atggtggaat taacgaaaac aacaactggt gtcacatccc gcagacaaaa gaggcagcgg
     7681 ctaactaaga ggcctgctga ctttctcgcc gatcaaaagg cattttgcta ttaagggatt
     7741 gacgagggcg tatctgcgca gtaagatgcg ccccgcattc ggtgattggc gcagcttggt
     7801 agcgcacttc gttcgggacg aaggggtcgg aggttcgaat cctctatcac cgaccaaatt
     7861 cgaaaagcct gctcagcgag caggcttttt tgcatttgaa gcccatgagg atgagaacct
     7921 ccgggggcgg ggaggttcga ctcgagcgaa gcgagagaac gttgcgccag caacggcccg
     7981 cagggcgagc cacgaagtgg cgagtaatcc tctatcaccg accaaattcg aaaagcctgc
     8041 tcaacgagca ggcttttttg catttgtagc tcatgaggat gagaacctcc gggggcaggg
     8101 aggttcgact cgacgtaggc ctgataagac gcgccagcgt cgcatcaggc aacggcaccg
     8161 catttattgt cggatgcggc gcgaccgcct tattcgacca acacaccaca cattttcctg
     8221 ccaactttgc gccccatatc tcactatcca ttaacggatt tgtgacagaa accattgtct
     8281 ttttttatat ttgcttaaat ctttttttgc gttacttatg gctggcgctg gcattttccg
     8341 ctgtgcgttt tagcgttcat ggtattaatc gctcagatat ccaccttttc ctcagatttt
     8401 tttcattaaa tgcataaaac ttaatcacca aatgttgcta aatattaagc tgattgttct
     8461 ggttatgttg gagtagcaca aatttctctg ctgcaaatag ctgtttgaag tttttttaat
     8521 ctttgtttgt ggattctcac cgttggtgta aatcccggtt ggctgtattg acgttttcac
     8581 attctgttga cagattgtag gtcacgaggg gcattttatg gaggatccgc actgttacac
     8641 tgatgttaat tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa
     8701 aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga
     8761 gcagaataat gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg
     8821 tggctatgac cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat
     8881 ctccggaagg gtttaacccg cagctgttta cctccggcac cacctatgac gcctcttccg
     8941 taccgcttta taaccgcctg gttgaattta aaatcggcac cactgaagtg atcccgggcc
     9001 tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta taccttccat ctgcgtaaag
     9061 gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg tgaactgaac gccgatgacg
     9121 tggtgttctc gttcgaccgt cagaaaaacg cgcaaaaccc gtaccataaa gtctctggcg
     9181 gcagctacga atacttcgaa ggcatgggct tgccggagct gatcagtgaa gtgaaaaagg
     9241 tggacgacaa cacagttcag ttcgtactga ctcgcccgga agcgccgttc ctcgctgatc
     9301 tggcaatgga cttcgcctct attctgtcaa aagaatatgc tgatgcgatg atgaaagccg
     9361 gtacaccgga aaaactggac ctcaacccaa tcggaaccgg tccgttccag ttacagcagt
     9421 accaaaaaga ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc
     9481 agatcgatac gctggttttc tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc
     9541 agaagaatga atgccaggtg atgccgtacc cgaacccggc agacatcgct cgcatgaagc
     9601 aggataaatc catcaacctg atggaaatgc cggggctgaa cgtcggctac ctctcctata
     9661 acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca ggcactgacc tacgcggtga
     9721 acaaagacgc gatcatcaaa gcggtttatc agggcgcggg tgtatcagcg aaaaacctga
     9781 tcccgccaac catgtggggc tataacgacg acgttcagga ttacacctac gatcctgaaa
     9841 aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg tttctccatc gacctgtggg
     9901 cgatgccggt acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg
     9961 cagactgggc gaaagtgggc gtgcaggcca aaatcgtcac ctacgaatgg ggtgagtacc
    10021 tcaagcgtgc gaaagatggc gaacatcaga cggtaatgat gggctggact ggcgataacg
    10081 gggacccgga taacttcttc gccaccctgt tcagctgcgc cgcctctgaa caaggttcta
    10141 actactcaaa atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg
    10201 acgaccataa caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcaga
    10261 ctccggcact gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagtcaaag
    10321 gctatgtggt tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa
    10381 aagccataca agactgatgg caaaggcaaa actgcctgat gtgctccgct tatcaggcct
    10441 acgaaaattc tgcaatgtat tgaatttgca cgattttgta ggccggataa ggcgttaacg
    10501 ccgcatccgg cataaacaaa gcgcactttg tcaacaatct gtatacccgg tggcgctgtg
    10561 cctctgcgtg cagcgccatc cggcagcaat acttcattcc ctgtccgata agggcgggaa
    10621 ttgatttgtg agcaatacag acacgcagtt ccaggctgcg ggtcactata gagaatccgg
    10681 gttatgttgc agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt
    10741 acccttctca catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggca
    10801 ggcgaacgcg ggatctcccc tgagcgtcac gcgcagctgc tggctgaact cggcttagat
    10861 aaaccgatgt ggcagcagta tctccattac atttggggcg taatgcacgg cgatctaggc
    10921 atttcaatga aaagccgaat tccagtatgg gaagagttcg tgccgcgctt tcaggccacg
    10981 ctggaacttg gcgtctgcgc gatgattttt gctacggcag tcggtattcc ggttggtgtg
    11041 ctggctgcgg ttaaacgcgg ttccattttc gatcacactg cagttggcct ggcgctgacc
    11101 ggctactcga tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac
    11161 tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat
    11221 ccgttaaccg gttttatgct gatcgacacc gccatctggg gtgaagatgg caactttatc
    11281 gacgccgttg cccatatgat cttaccggct attgtgctgg gtactatccc gctggcggtt
    11341 atcgttcgta tgacgcgctc ttcgatgctg gaagtgctgg gtgaagatta catccgcacc
    11401 gcgcgcgcca aagggttgac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg
    11461 atgctgccgg tagtgaccgt tatcggcctg caggtgggaa cattgctggc gggggcgatt
    11521 ctgaccgaaa ccatcttctc gtggcccggt ctgggacgct ggttgattga cgcactgcaa
    11581 cgccgcgact atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc
    11641 gtcaacctgc tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag
    11701 taaggggaca tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg
    11761 accccgttac aggagttctg gcactatttt aaacgcaaca aaggcgcggt tgtcgggctg
    11821 gtttacatcg tcatcgtgct gtttatcacg atctttgcca actggattgc gccttataac
    11881 ccggcggatc agttccgcga tgcgctgctc gccccgccag cctggcagga aggcggcagc
    11941 atggcgcact tgctgggcac cgatgacgta ggccgtgatg tgctgtcgcg gctgatgtac
    12001 ggcgcgcgct tgtcgctgct ggttggctgt ctggtggtgg tgttatcgct gattatgggc
    12061 gttattctcg gcctgatcgc cggttacttc ggcggcctgg tcgataacat catcatgcgc
    12121 gtggtcgata tcatgctggc gctgccaagt ctgctgttgg cactggtgct ggtggcgatt
    12181 ttcggcccgt cgattggtaa cgccgcgctg gcactgacct tcgttgcctt gccgcactat
    12241 gtgcgcttaa cccgcgccgc cgtgctggtg gaggtgaatc gcgattacgt taccgcgtct
    12301 cgcgtggcag gtgccggggc gatgcgtcag atgtttatta acatcttccc gaactgcctg
    12361 gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggccgcc
    12421 cttggcttcc tcggcatggg agcgcagccg ccaacaccag agtggggcac catgctctcc
    12481 gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga ccttcccggg tctggcgatc
    12541 ctgctgacgg tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc
    12601 aaactgaagc agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca
    12661 tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca
    12721 gggcgaagtg gtcgggattg tgggtgagtc tggctccggt aagtcggtca gttcactggc
    12781 gattatgggg ctgattgatt atccgggccg cgtaatggcg gaaaagctgg tgtttaacgg
    12841 tcaggatttg cagcgtatat cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc
    12901 gatgatcttc caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat
    12961 catggaagcg attaaggtgc atcagggcgg taataagagc actcgccgtc agcgggcgat
    13021 tgacctgctg aatcaggtcg gtattcccga tccggcatcg cgtctggatg tttacccgca
    13081 tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc
    13141 aaaactgctg attgccgatg agccgaccac tgcgctggac gtgaccattc aggcgcaaat
    13201 catcgaactg ttgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca
    13261 tgatctggcg ctggtggcgg aagctgcgca taaaatcatt gtgatgtatg caggccaggt
    13321 ggtggaaacg ggcgatgcgc acgccatctt ccatgcgccg cgtcacccgt atactcaggc
    13381 attgctgcgt gcgctgccag aatttgctca ggacaaagaa cgtctggcgt cgttgccagg
    13441 tgtcgttccc ggcaagtacg accgcccgaa cggctgcctg cttaacccgc gctgccccta
    13501 tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg acgggcgcca
    13561 gtccaaatgc cattacccac ttgatgatgc cgggaggccg acactatgag tacgcaagag
    13621 gccaccctgc aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag
    13681 aaaggcatgt tcgcgccgga acgtctggtt aaagcgctgg atggcgtttc gtttaacctt
    13741 gaacgtggca aaacgctggc agtagtgggc gaatctggct gcggtaaatc gaccctcggt
    13801 cggttgctga cgatgattga aacgcccacc ggtggcgagc tgtattacca ggggcaggat
    13861 ctgcttaagc acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc
    13921 cagaaccctt acggttcgct gaatccgcgt aaaaaagtcg ggcaaattct tgaagagccg
    13981 ctgctgatca acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg
    14041 gcgaaagtcg gcctgaaaac cgagcactat gaccgctatc cgcatatgtt ctccggcggt
    14101 cagcgtcagc gtatcgccat cgcccgtggt ctgatgctcg acccggatgt ggtgattgcc
    14161 gatgaaccgg tttccgcgct ggatgtttca gtgcgcgcgc aggtgctgaa tctgatgatg
    14221 gatttgcagc aggagttggg gctgtcttat gtctttatct cccacgacct gtcggtggtg
    14281 gagcacattg ctgatgaagt gatggtgatg tacctgggcc gctgcgtgga gaagggaacg
    14341 aaagaccaaa tcttcaataa cccgcgccat ccgtacactc aggcgctact ttccgcgacg
    14401 ccgcgcctga acccggacga tcgccgcgag cgcatcaagc tcagcggtga actaccaagc
    14461 ccactgaatc caccgccggg ttgcgccttc aacgcccgct gtcgtcggcg cttcggcccc
    14521 tgcacccagt tgcagccgca gctaaaagac tacggcggtc aactggtagc ttgttttgct
    14581 gttgatcagg atgaaaatcc gcagcgttaa ctcacaaaac cggggccatg cgctccggtt
    14641 tactcaatca gcttcaacag tggcgacaag catagcaaaa tgccgtacag cagaatcagc
    14701 caggctttaa atccgcgaag tttttccagt tgtgcgactt tatagatcag gaaaaacgga
    14761 atgaggcacg acacaatacc atataacggg cttcccaact gaaagaacac cagcaccgat
    14821 acacgaaacg aaacccaaat cgtcaacgta atgacgataa aagcgcagat cgccagagtc
    14881 agcacgcgtg agttaatttt cttggtatca ataattcggc ttaacagatt gagaataatg
    14941 cctttaatgg cctcgtggaa accgagataa atgccaaaga atgcggtcag tacggcaaag
    15001 atattaagca ccgtagaggt gatatgaatg atatgcccag ggatcacctg cgcggccagc
    15061 gccagtgctg agatattttg ttcaaaggca gaaacggctt cttcgtggct aattgagaag
    15121 gtaaacgaaa aggcaaaaaa caggatcacc gcgatgagcg tgatataact aattcggtgg
    15181 gtacgcagcg cgagccgggt tgccagtact ttatccgctt cccgtttacg ataggcaata
    15241 ttcattgggt taagtacctg aataaatact gcagaaaaga agcaaaatgg aatggtaagc
    15301 aagacatcgc ggaaaaagac ggaggcttgc gggaaggcgg ttatattggc gaaattccag
    15361 tgcgggatca tcgcaaaacc gaacacgaca ataatcccta ctttgaccac caccattggc
    15421 ccggaaatct taaacagtaa tcgttcacca ccagacgcaa tcgccaccag tacggcgaaa
    15481 atagcgactt tataaagtag agattgtgaa agatcggcat cggttaaacc gaaggttttc
    15541 aggtacgagg cgctgtcgaa aaccacggag agagagtaga taaaaatccc gtggataatc
    15601 atcaaaaagt agataacccc gaggaaaatt ccccagttct tccccaggta atgactgata
    15661 atatcggtgt agtcattaca ggaatcgctt tcagaaaggg tttttaaata aatgtcctgc
    15721 actacccagg tggcaggata agcaatgatc gctgcggtaa taaataccca aattcccttc
    15781 aagccaattt gtactggcat cagcacggtt ccggcaccaa tcgccatgcc tatgcataat
    15841 aaaacccagc cgaagtcgta gcgtgtaaac ggcaatttct gattgtgttt cgatagtgtg
    15901 ttgtgctgca taaagtcctg tccagaaaga agtcggcctc gcaaaaatga ataaacccag
    15961 tttctcttgc gcaacaggtg gagaagtcga atacgcagta tgtaaaaaat gaggcaacag
    16021 attcactggg gaggtcgcga ggaggcgcaa ttctacaaaa ttgcgcattt cactaccagt
    16081 ctggagtcag cccggatatg aactgcaact attgcaaata aagaaattaa gctggaaggc
    16141 atcgcgcaat gaggaagatt gccgggtgct gacagtaaaa agcaatcgta aaatcagcgg
    16201 caagccgaaa gggttccggt gaggcgcaat gttgcggggg ctttatccct ggtggcattg
    16261 gttgctggag agagaaaacc cccgcacgtt gcaggtatgt acctgacaac ataacggggg
    16321 ctaatcttga acctagacca ctcaagaata gccgcgaaac gttgtcatta caacacaggc
    16381 ggctatatga cgctcgcaga
//
LOCUS       DAESHI010000090        16270 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.091, whole genome shotgun sequence.
ACCESSION   DAESHI010000090 DAESHI010000000
VERSION     DAESHI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16270
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.091"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(164..979)
                     /locus_tag="KW011_003044"
     CDS             complement(164..979)
                     /locus_tag="KW011_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661324.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(1222..2271)
                     /gene="yahK"
                     /locus_tag="KW011_003045"
     CDS             complement(1222..2271)
                     /gene="yahK"
                     /locus_tag="KW011_003045"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HBH7661325.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMIGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAEKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIADSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(2648..4029)
                     /locus_tag="KW011_003046"
                     /pseudo
     CDS             complement(2648..4029)
                     /locus_tag="KW011_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            complement(4039..4989)
                     /locus_tag="KW011_003047"
     CDS             complement(4039..4989)
                     /locus_tag="KW011_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HBH7661326.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTDVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFSPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(5132..6550)
                     /locus_tag="KW011_003048"
     CDS             complement(5132..6550)
                     /locus_tag="KW011_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HBH7661327.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(6550..8097)
                     /gene="fdrA"
                     /locus_tag="KW011_003049"
     CDS             complement(6550..8097)
                     /gene="fdrA"
                     /locus_tag="KW011_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HBH7661328.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPTLEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(8087..8950)
                     /locus_tag="KW011_003050"
     CDS             complement(8087..8950)
                     /locus_tag="KW011_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HBH7661329.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(8990..9595)
                     /locus_tag="KW011_003051"
     CDS             complement(8990..9595)
                     /locus_tag="KW011_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HBH7661330.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            9853..10350
                     /locus_tag="KW011_003052"
     CDS             9853..10350
                     /locus_tag="KW011_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBH7661331.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALTAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            10442..11374
                     /locus_tag="KW011_003053"
     CDS             10442..11374
                     /locus_tag="KW011_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH7661332.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(11416..12504)
                     /locus_tag="KW011_003054"
     CDS             complement(11416..12504)
                     /locus_tag="KW011_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH7661333.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(12646..>16270)
                     /gene="ehaA"
                     /locus_tag="KW011_003055"
     CDS             complement(12646..>16270)
                     /gene="ehaA"
                     /locus_tag="KW011_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HBH7661334.1"
                     /translation="GFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIHVKGT
                     SRLTYLQGNVSGEGSQVNSKTFFMGVYGSYGGNQYLSVNNGGEVNASEKISLGYYDQN
                     SDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKTSEKK
                     ITLNHTDKNATISADIVSDSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGALGITQ
                     NIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYIDVASG
                     AVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFDEFAY
                     TDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSLVTVGKGAHSISTLGFSGGTVDF
                     GALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDDGNSA
                     IKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTSGVNN
                     DGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQTVSL
                     SNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNGSADS
                     LLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSANVLVS
                     HAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAGNVLV
                     VKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLNGIEL
                     IHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDPMPNP
                     EPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGNTYYT
                     DMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSDSWHV
                     GVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQYSWF
                     DNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTWMGVK
                     ADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLHNSKD
                     FSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAMVGIK
                     WQF"
BASE COUNT         3772 a         4392 c         3855 g         4251 t
ORIGIN      
        1 actgctggtg cttttttaag cgaagtgtcg ccgatcacaa caaaatatcg gcgaaaaggc
       61 ggtggaatgc catttggcat acggatatac agatgaaatg attacctgct ttgcgattca
      121 ctctctggtg gaataacaac ttgcaggtgg aaccctttct gaactaaaga gtgtagcgga
      181 ttttaacgtg cttatttaca ggataatatc tgtcacctaa ttcctgcact ctattagata
      241 tccattcatt taatcgtgca tcatctctgt atgtttttac gtccagaaca gagagcaata
      301 acatcaccag tgattcagga aaaacagggt agatattttt taagtgtaat aataatccat
      361 gcgcaagcgc ctgcttaatg ggcgcgtctg tctccggaat agacgacatt tcgcgaagcc
      421 atttttccac atgcatacac tctttccctt tagggaaaag tctcagcaaa ggcatatggt
      481 gttcaagaag aatacatttg caatctgttc gcactgaatt gataaactgc actaatttct
      541 taagcccggt aaagcgtcca gcctgcacat ttgctttaat atcaagaaac gcatcatcct
      601 ggtcatatac ccaaactgtc cctttttgat tttcaagtat gcgaaaaaca aacttaaccg
      661 ggtgatcagg gtaatccatc cagtcataaa atcctgggaa atcattaaac tgcacattta
      721 tggtgagtgc gttatcgtct gaccactcac agtctcgtac ggcagccatg agatgaatga
      781 gtttgtcgtc atcgactaaa tcatcacgac aatcataagg ataatcataa tattgtttaa
      841 tcaaaacctt gaatatatcg acacaggaaa ccagggagct tttgatatcc tctggtaact
      901 gattgttttt aaccgtatta agtatgcttt gttgcgtata tggcatcaaa ttattgtgcg
      961 gagcttttaa tgatatcatc gcgatctctc ttaaataaaa ataaacgtcg cagataataa
     1021 agcgctgatt aaaccaggca ctatcagaaa tcgctcataa ctaacgtgag aagtattgtg
     1081 tactggaggg agttaattta acaatttgaa tttacgttat aggttttact gctactttga
     1141 tattatttcg ctctccttaa ctaaaaagtt aagtggagag cgaagttaat atgttaaacc
     1201 acagcgtatt tattaatttt ttcaatctgt tagtgtgcga ttatcgataa caaaacgata
     1261 tttcacatca ccacgcagca ttcgctcata ggcttcatta atttgatcgg cccgaatcat
     1321 ctctatatca gccacgatgc catgttcggc gcaaaaatcg agcatctcct gagtttctgg
     1381 aatgccgcca atcatagaat cggctatcgc acggcgtttc atgatcaggt tgaaaacttc
     1441 cggcgattta tgcggtgtcg caggcgcacc aaccagcgtc atggtgccat cacgcttcag
     1501 caaggtggta aaatcgtcga gattatgtgg cgcagctact gtattcaaaa tgaaatcgaa
     1561 actcttcaga tgagccgcca tctcatcggc attgcgtgag ttaacaactt catcggcccc
     1621 cagggctttt tccgcttcgc gttttgcctc agaagtggta aatgccacca catgtgcccc
     1681 catcgcgtgg gccagcttaa tccccatatg tcccagaccg ccgatgccga ccacgcccac
     1741 ttttttaccc ggcccggcct gccagtgacg tagcggcgag tacgtggtga tccctgcaca
     1801 caacaaagga gccaccgccg ccagctgctc ttgcgggtga cgaatacgca gaacatatcg
     1861 ctcatgaacg acgatctgtt gtgagtagcc gcccagagta tggcccggtt cgtccggcgt
     1921 cggcgagtta taggtgccga tcatgtgatc acagtagttt tccagcccgt cctcacactc
     1981 ttcgcaatgt ttacaactgt cgacaatgca gccgacaccg accagatcgc ccggcgcatg
     2041 tttttctacc tgatcaccaa cggctaccac acgccccaca atttcatgac ccggtacgca
     2101 ggggtaaacc gtccccgccc actcggaacg gacctggtgg agatcggaat ggcagacgcc
     2161 acagtaagcg atttcgattt tgacatcatt cggtcccggt tcacgccgtg tgatatccat
     2221 cggttcaagt ggttgtttag cggaatatgc accaacagct ttgatcttca ttgtgtttac
     2281 tcctgattag ctatgtgtat ttggcgacgc ctgatatgtc atcgcggcaa aacgcgtcca
     2341 ttgaatatag ccaatattta aaccacccga tccagcccca acatgccgcg aacctgaatg
     2401 aaacagagca gcaacgttcc ccataaggcg agcgcctata agtgtgatat ttgctatccg
     2461 taaaagacta aaatcggcaa aactgatgat cttttcacgt agacaaatag ttatctacac
     2521 aacgtcaata agggcatatt cctcaaagat aatgtccatt tctgccgaca ctatcatatt
     2581 gaggtcgccc cactccctgg ggcttaaatc tcttacaatt caccgttgca ggcgtttcaa
     2641 atgcgcatca acctgccaca ctcccccaca ccagttgccc tttatggaac gttgcggttc
     2701 tcggcgagat acgcgccacc gcctcggcgg aacaggaggc gtccaccagc acaaagctgg
     2761 catcatcctg cgcttttggc catacacgct cgcctttttc attcagcggc aatacatcgc
     2821 cagtggctag aacagcgaac gggagaggtt ctgttcgtta ggacgaatat agagctgcgc
     2881 gtagagattg gctttttcca gcatgtcgcc cagaccataa ggcgaccagt ggtcgataac
     2941 gctgtcagtg ccagtcatca cttttacgcc tttgtcgtgc aactgtttga gcggcatatg
     3001 cagcgtgcca atcggcaccg tcgaggcgat agaaatttgc tgcgccgcca tccgattcgc
     3061 cagcgcatcg acctgttgtt cgttgagcgt agccagcgca aaggcgtggc tgatggtcag
     3121 cttacctttc agttgcggcg ttttctctac cgtttcaacc atataattga tggctgccac
     3181 gcccgacgga gtggtttcgt gcaggtgaat atcgacacct ttgtcgtagt ccagcgcaat
     3241 ctgaaacatg gtgtcgaggg atttttccat cgcgccatcg acactggtcg ggtccagccc
     3301 gccgacgtaa tgcgcacccg cctgcatcgc ttcgcgcatt aagggttccg atttcgacag
     3361 cagcaaaccg tgctgcggga aggcgacgat ttcacactcg aagcccgcct gacgccgcgc
     3421 cagcaccgcc tgcaaatttt gcagattttt taggccggaa accggttcga tattgcagtg
     3481 gctgcgggca atggtggtgc ctttcgactg caatagatcg atcagttttt ccgcccgctc
     3541 ctgcgtatac ggttgtaact ccggcagcat tttctgcttg agtttgatca tgtcctggat
     3601 agtggtgcct gccggacgat tgagcgagcg ccacggcccg ccgtagaagg ttttgtcgag
     3661 atgaatatgc atgtcgcgag tggtgggtag catcaactta ccgccagcgt catagtgcgg
     3721 cagcgtggcg tccggatgct gcttgttctc gcgcagggcg acaattttgc cgtcctgaat
     3781 ctccacggtc tggcgcgccg tgcgggtctg caccgccaca ccgttttcgt agtcaaaacc
     3841 ggtttccagc agcacgttat cgagatagta atgcgggtca gtgattttca tggtgttgtt
     3901 cgcttcgcag tttagggtgc cagctaccgc cgcatgggcg agcggcacat aggtaccaaa
     3961 cagcgcggcg gcggtgacca tcttaccgct ctggctcaga aattcacggc ggctattgct
     4021 ttctttcatc ctacgtcctt acgttttaat aatatgggtg cccgtttcac cgcgcagcgc
     4081 cgcaggcagg cattccggcg tggtgataat cacttctttg ccgccctgct ccaggaacgt
     4141 caggctggcg atgatttttg gcaacatgct gccgggagaa aaatgccctt cctgcatata
     4201 gcgagtcatg gtggcaatat ccacccgatc cagcgcctgc tgctgcggtt taccaaagtg
     4261 gatacagact ttttcgacgt cggtggtgat caccagaatg tcggcgtgaa tttcacgggc
     4321 cagcagcgcg gtagagagat ctttgtcaat aaccgcgtcc acgctttggt aatcccccgc
     4381 ttcagtacgc actaccggaa ttccaccgcc gcccgcgcca attacgacaa aaccttgttg
     4441 gatcagcgcc ttaatggcgg gtgcttcgac aatacgtttc ggttccggcg aggcgaccac
     4501 gcgacgatag ccccgtccgg catcttcaac aaaacaccag tcagggtttg ccttttgtag
     4561 tttgtcacgc tggctttcac tgaagaatgc accaatgggc ttggtgggat gggcaaaacc
     4621 tggatcgttt ttatccactt ccacctgagt taccacggtg acggcttttt tctcgccgtg
     4681 acgcgccagt cggttattca gcgcctgttg gatcagatag ccgatgccgc cctgcgtatc
     4741 tgccacacag ttcgccagcg gcgttaaggg cagcccttcg cgctcgtggg caatctccgc
     4801 acggcgtaaa tccagcccga cctgcggccc gttaccgtgg gtcagcacaa tgtcgtaatc
     4861 ggaagccagc atttccagca ccgtatcggc cacggctttc accgcctccg cctgatgctc
     4921 aatcgactgg ctggcgttat ctttgataat gctgttgcca ccaatggcaa cgaccacaag
     4981 ctctttcatg ttgttatgtc cgctacagag ggtgatgagg ttatgaccaa cgccagactg
     5041 cctgatgcgc tacgcttatc aggcctacaa ttctcgggcc atccgtaggc cggataaggc
     5101 gttcacgccg catccggcat aaacatgtaa gtcagacgcc gtaacgttcg caccagccga
     5161 gaatggcttt ctcgaagcaa gcgaatggtg gatgcacaat gcccgcgcca atcatgccga
     5221 cgcccgcatc tttatgggcg atggcggtgt tgatcaccgg cagaatgccg ctgctaccca
     5281 cgcgggtgat gtcgattgcc gacggcacgc ccataaaacc gagcagcgga atggtgacgt
     5341 tggggttttc accgagagtg atttcacgca tctgacggga gaaatcgata gcctcttcca
     5401 ccgtgccgcc caccagcgcg acgattgcag gcgcggtcgc catagcaaat ccaccaatac
     5461 cgtaggtttc ggtgatggcg ctgtcgccga tatccagccc cgaatcttcc ggcttataac
     5521 cggcaaacat cgggccgatc acctgctgcg ccgggccggt aaaccattgc cccggcaggc
     5581 cgctgacccg caggccgaac tcgacgccgt tacgcgccat ggtggtgacc acggtgctgt
     5641 attcgatgcc gtgcgccgca tccatcgccg ctttacacat cgccatccac gtcgggccgg
     5701 agaagtagtc gctgctggcg acaaactcga acacttcgcg ctgttgctcg acggaatagc
     5761 ctgcctgaat gatccccggc gtcagtgcct gaatcagcag cgtcgtcccg gcgttattgc
     5821 ggttatggca ctcatcgccc atatgcagcg cctgcgccag cattaagcgc agatcgatct
     5881 caccgataat tttcatcgcg tcgcgcagca ttggcccctg cacatcgcgc atccagttga
     5941 ggcggtcgat cacgctctga tcgttggcac ccatacgcaa aatcttcgcc atctgctcgc
     6001 tcatattggt gtaagcgatg ttgccgtagg ttttgttttt cacgatgtgc ataaacatcg
     6061 aggccgaggt aacgcccgcc atcgatccca cacagtcgtg ttcgtggcac ggcgagaagg
     6121 tgatctcccc ggaggccgcc agttcagccg cctcgtcgag atctttcgcc agtccttcga
     6181 acaccagcgc tccggtgacc gcgcctttca tcgcgccgca cattttttcc caggtgatcg
     6241 gcggcccggc gtgaagaatg gttttcggcg tcatgcccgg caccacgtta atcgcctgat
     6301 caaaaccaat cagcaccgga tgcgactgga taatgcgctc cagcgcctgc tggttagcgg
     6361 cggcgatttt gtctgccagc ggcgaatcgg caatgttatc cagcgcctgc accacctgca
     6421 tattgccctg ccccggcggc gtccagtcga gttgagtcac ttctacatgc tgctttttca
     6481 ggtcatcgct aaacatggcg atgccgacgt taataacgtt caatggttgg ctaaacagtg
     6541 actggctcat caggcttcct cccctttgca gataaattca cgcgccagca atccggtatt
     6601 ggtgctgctg ctcgccagaa tcactccggc atcaagcagc atctggcttt gttgttctaa
     6661 cgatggcgta tcaagatcgg tacccagcac ataggcgaga atgatcaact cgcgtccttc
     6721 ggcagcggcg atcgctttcg cttctttgat cgcctcgatg gtggagccga ccggatcttc
     6781 atgcgatcca aatccgagca caaaatccat cacgatcacc gccacttctg gatcgcgcgc
     6841 ctcttcgatc aagcgactga tgcggttggt ggggtcaatc attggatgcg gcttgccgtt
     6901 cgtgaagtcg tcatcgccaa agtcgaggaa ggtgtgtttg atgctgcggt tgatatcttg
     6961 caggcggaat tccggatcgg gctgaatgtt gctgtagaca tcgccatgtt tttccatcac
     7021 cgcgaacatg gtttcgtcgc acagcgtgcc gccgcagaac aggccacgaa tgtatttctg
     7081 ctgcggttgc agacgcgcac gcacatcagc aatcaacggc tggttaagcg tatgcaggtc
     7141 gagattttcc tgtttcacgc cggagagcat caccgctttt agcgccgcct ctttgctgcc
     7201 gcgggcaaac tgtagcccct gctcatccac cggcgtttca acgcgaccga ggaagcagac
     7261 caccaccggc ttgcggcagg cacgtgcgcg ttccagcact ttgcgggcca ccgcaggcgc
     7321 gggaggtttt gaaatcagtg cgataatttc agtttgcgga tcgttttcca gcatcccgat
     7381 ggcgtcgagc atcatcaggc cgccgatttt ctcgctcagg tcgcgcccgc cggtgccaat
     7441 cagttgtgaa acaccgccac caaattcatg aatgcggacg ctcagctcct ggctgccggt
     7501 gccggatgcg ccaacaatac cgatattgcc gcgacgcacg gcgttaccaa aacagagcgc
     7561 cgcgccgtta ataatcgccg tgccgcagtc cggccccatc atcagcagcc ctttttcgtg
     7621 ggccaactgc ttgagcgcca gttcatcttc aactgagacg ttatcggaaa acagcatcac
     7681 gttgagatcg ttttgcagcg cctgacgcgc ttcgcgagcg gcaaacagac cgttgaccga
     7741 aatcaccgcc aggttacttt ccgggatatg ctttttggcg ctggcaatag tggcgtaacg
     7801 cgcctcgtgc gagccgcttt gcgctttggt gttgaacagt tcttcaatct ccaccagcaa
     7861 ctgctcgttg tccgcacccg atttaccatt gatgacaatc atcaggtcgc cgtttttcgc
     7921 ctgttccagc gtcggcgtca gcagtcctaa atttttcagc acacctttgt tcatttcggt
     7981 cgccatcgcc acaaatgcct gctcgacgcc gtcgagttta tttgcacgcg tggagataga
     8041 catcagcgag acagaatcaa aataggtatt cggtttaatg actattttaa ctgacataat
     8101 tccctccgta ggtttggctc agcgcacaac catcggccat gccatacagc atgtcgcagc
     8161 cggaactgga gccaatatgt ttaatttttt ctattgcctg agttgaattc ccagggacgc
     8221 cataaataat gttgtgaata aagcgatgaa tattttcccg gcagcgttgt tgtaatgcgg
     8281 cttccagcgt tatggcactt aataacgtgg tgttatttct gccgcgtagc agaccgcgat
     8341 aaaattcctc tttgtatttt tctgccggat gcccgggaat aaataaaata agcgccagac
     8401 ctgttaaata atcatcggct gatggcgtaa ggccaatccc taacccaatc atactggcga
     8461 ccgctgcggc gatattttgc ttatcgttta ctgtctgaat aactgcccgt cgtttaatat
     8521 gtaattcttt atttaacgcc tgataaaacg gattatcgcc gcgataatta aacagggttt
     8581 cattttcccc caggtgctga tgaataatgt cgtgccactg tagccaggcg ataaggtgaa
     8641 aattcacagc ggtcatttgc aaggttgggg acagccaacg ccgacaacga ctcatctcta
     8701 tatgaagatg ttgaccaacc gtaatacctt gatcgtcaaa ctgaacctta tcaccatgcc
     8761 ggaacagatc atcaaagtga gtgagtgcca accgacaact gtttggcgca ttgtcgtact
     8821 cttcacaaag cagcgtcagc aactgctggc gagccgggag tgcgatattt accgctctgg
     8881 caaagacctg ttcaacctgt ccttgccccc gctgcgccag aaaatccgca tccgcagtta
     8941 gcgcccacac acaccgtttt tctggtgtgt tgatagccag cctcccggtt tatgcacctg
     9001 cggcaatcag taactgcgca atctcttcaa agccccgttc ccgcgccagt tccagtggcg
     9061 ttttgccata tttatcggtc agatgcgggc tggcaccgtg ttccagcaat aactgcacaa
     9121 tcgcctgctg tttaatacca ccatcattaa gcacaatcgc ttccagtagc ggcgtccagc
     9181 ctacatggtt ggtctggtta acgttaatct ccgtatgcgc caaaagctct tttacaatac
     9241 ttaaatggcc tttttcacag gcaggcgtca ggccgacacc gccaaaacgg gttacgcaat
     9301 taagatcggg tttagccggt aaaataattc gtagtagcgt taaatcatcg ttcagacagc
     9361 taattaaaaa aggatttaaa caggtatgat cttgcttatt aatatccgct ccggcatcaa
     9421 ttaatgcctg aacgcaagca tattgctgat ataaacttgc cagcgtaatt gccgttttcc
     9481 cctgacgatc gcaggtatta atatcgacac ccagcgcaag gcaggttttt actttatcga
     9541 tatcaccctg ttgtgcagcc aataaatagt cggcaggtag attttttata ctcattcaca
     9601 ttcccctggt ttttgtggct gttttcgatg aattgagaat agcaacgata atctgataaa
     9661 agaacccgct taaatttaat tcgccgtatc tcgccttttt gttgaataat attcaacaat
     9721 cttttcgcag tgtgcggtag tgcaatattt gtttctttga cccgataccc tttcactcaa
     9781 tcaggtttta tttctctata gtctgcacat gtaagagtag ctttcgtcca tgtctaacca
     9841 gaggggaaca agatgaacgg actcacggca acgggtgtca ccgtcggtat ttgtgcaggg
     9901 ctttggcagt tagtgtcttc acatgtcggg ctctctcaag gatgggaatt attaggaacc
     9961 attggcttcg tcgccttttg cagtttttat gccgcaggcg gcggtaaatc ggggtttatc
    10021 agaagtctgg cggttaatta ttccgggatg gtgtgggcgt ttttcgccgc gctgactgct
    10081 ggttggctgg cctctgtaag tggactgtcc gcgttctggg cgagcgtaat tactaccgta
    10141 cccttctcgg cggtagtggt ctggcaaggg cgtttctggt tactgtcctt tattcccggt
    10201 ggctttctcg gcatgacgct gttttttgcc agcgggatga actggacggt gacgttactg
    10261 ggttttctgg cgggtaactg cgtggggata atttcagaat atggtggaca aaaattaagc
    10321 gaagcgacga ctaaacgcga cgggtattaa aagttattca aaaatgatta tctatcttta
    10381 accgatacct ctacagtcat agctggagaa attcatagta gataatggcg agaatggttt
    10441 aatgaactca atttttaccg aagagaatct gttggcattt actaccgctg cgcgttttgg
    10501 cagcttcagt aaagccgcag aagagttggg tttaaccact tccgccatta gctacaccat
    10561 caagcgtatg gagacggggc tggatgtggt gctgttcact cgcagtaccc gcagcattga
    10621 gttaacggag tccgggcgtt attttttccg caaagctacc gacctgctga atgattttta
    10681 tgccatcaaa cgcagtatcg atactatttc gcagggcata gaagcgcgtg tgcgcatttg
    10741 tattaatcag ctgctttata cgcccaaaca caccgcacgc ttgttacagg tgctgaaaaa
    10801 gcagtttcct acctgccaga ttaccgtcac caccgaagtg tataacggcg tctgggatgc
    10861 gattattaat aatcaggcca atatcgccat cggcgcaccc gatacactac tggacggcgg
    10921 cggtattgat tacactgaaa ttggcgcgat tcgctgggct tttgctatcg ccccggatca
    10981 cccgttagcc tttgtgccgg aacccatcgc cgaaagccaa ctgcgtctct accctaatat
    11041 tatggtggag gacaccgcgc atacgattaa taaaaaagtg ggctggttgc tgcacgggca
    11101 ggagtcaatt ctggtgccag atttcaacac caaatgtcag tgccagattt taggtgaagg
    11161 aattggtttt ttgccggatt acatggtccg tgaggcgatg acgcaatccc tgctggttac
    11221 ccgacaaatc cataatccgc gccaggattc gcgcatgtta ctggcgactc agcattctgc
    11281 tacgggtcag gtgacgcagt ggattaaaaa acaatttgcg ccaaatggca tattaacggg
    11341 gatctatcag gatttactgc atcgggagaa ttaatcgctg ccaacctccc gatgcgctgt
    11401 gaatagcggt ttacatcaac cacctgcttt cattacccat tcagagataa atttattacc
    11461 cggtactggc ggagagtaca aatagccctg caaaaagtga acgcctttac cgatcattaa
    11521 atccgcctgc tcctgggttt ctaccccttc cgccacgata ctcagaccag gcttacgcgc
    11581 tagttcgaca atattgtcca caatatgacc ggagatttcg tcgacactcg ccatttgcac
    11641 aaatgactta tcgatcttaa taaaatcgac cgggaacgcc tgcaagtaac gataggtcgc
    11701 ataacccgta ccaaagtcat ccagcgcaaa ggtaatgttg tgctgatgaa ggctgtcaaa
    11761 tatcgctctg gcttctggcg ttaccggaat agggttacgt tccgttagct cgagaaccag
    11821 cttgatttta tcgttaccta atttcttaac caggttcaga cactcttttt caaatcccgc
    11881 tgccaaaaaa caacccgccg agacgttgat gccaatatgg aaattgtccg gcagcaaatg
    11941 ttttaccggc atcagaatat ccgcagtctg tttcatcagt tggcgggtca ttatgacaat
    12001 aagaccggat gactccgcca gaggaataaa ctgatccggt gggataattc ccgtttgtgg
    12061 atgttcccag cggacaagta cctcacagcc cgtcagtacc ccagtctgcg cgcagaaaac
    12121 cggttggatc cacggtttga attcgtggtt ttccaacgcc agactgatgg cttcaggcgt
    12181 gacgatatgg tgacaacggt tatcagggat ataaaaatta tttttatttc ccgtgccact
    12241 gatgacttgc ggatgatatt gaaaaaagat atcccgccag aaggtaatat cactctggat
    12301 gcctagtttt tcatacagct gcttcttttg atgtgaaatt gtctttgcac tgcgatttct
    12361 gtactgtgag atttctgtaa ccgacattcc ctccattagc agcttgagta aaagccgttc
    12421 tttttcggac acgataccag gcaacgtcaa attcaccgtt gccccgaact cttccaaaaa
    12481 aacactcaaa tcatgcgaag ccattgaaca caccttgatt aaatcttgtg cgccagctaa
    12541 atctgcgagt cttcactctg actgataaag ctggctgcaa gaaaatgaca cagagagccg
    12601 aattatctcc cgttcggctc tctctccttt tactcattat tcagatcaga attgccactt
    12661 aatgccaacc attgcagagg tgtcattata tcccctatcg gcaacctgaa cgcccacatt
    12721 cccccagaca ttcaggttgg catttagctg tccttccacc ccggttttta tctcagcaat
    12781 atttcgggct ccatcctgag tgacggacac gccatccata ctggtgctga aatccttact
    12841 gttatgcagc cagttcactt ctacaaacgg ctggaactcg cgggatttac cgtcatccat
    12901 tttatggtgg ctcttcagcc aggtttttac gccaagtcgg gtttgaacat tgccatcacc
    12961 gttgctatga acgagggttc cgttgctttc gcggtgctta tcggctttga ctcccatcca
    13021 ggtaacctgt gcttgcggct gaacatacca ttcattacgc gttccctggc tgccattaaa
    13081 ttcagctaat ttgtgtttgt atccagcttc cagtgaagcg gtaaatcctt ttgatttata
    13141 ggattcactt tgtaagtcat cccctttcac tgtgttatca aaccagctgt actgcgccca
    13201 actgtcgaga tacgcgccat tacgcgactc gtcatcggca taccaggtgg catagaggcc
    13261 ggtgctatat ccgttcacac tcgcttttgc acgataaccg gttcgcgagg aaatggtttt
    13321 gctgtcgctg ttgccatatc ccgccatgac cccaacatgc cagctgtcgc tgccgttttg
    13381 gctccactgc gcgacatcgc ctcccagttg cagaacatag cgattgcttt gggttttcag
    13441 ctggccgctg ccatcacgcc atttattatg accaccttca tggcgcatcc acatagtggt
    13501 ttgtttctgc tcacccgtca ccatgtcggt atagtacgta ttacccagac gctcatgcag
    13561 acgcgtggtg aacattgtat tcgctgctgc aaggttcgca atgtagctac ccgcctccgg
    13621 tcgcaggtca ttatccacat tcagatccgg acccggcgta ggtgtcgggt tagggttcgg
    13681 ctctggattc gggtttggct ccggattcgg catcgggtct ggctccggct gcagttcagg
    13741 agaatcactg ccgctggtca gataccagtt accactattt gccccttgtc cacgcgcgag
    13801 agtgtagtca tacgcccccg caacgatacg accagcctga acaaattcgc cctcagactt
    13861 accgtctaca tggataagtt cgataccgtt aagggttttc gcacctgtac cgccagcgtt
    13921 attcaccgta acggcagtcg tgccgctagt atcgccctcg acaaccaatt tatcggttac
    13981 tgagtcatcg ccattcagta ccgtattcat tactagttga ccgttattac cgtggtagtt
    14041 ccccttcacc actaacacat tgccagcttg ctgtcctgac attccggtca tcagcgtacc
    14101 gttgttggtc agattgccgc ccagggtgta attcacagac gcagccgctc ttttttcagc
    14161 gctattattc aacgccaggg taccgttgtt ctcaacgttt gcgctaccca atccctgcgc
    14221 atgacttacc aggacattag ccgaattagc aatgttcaca ttcgctgtca ggttgctgtt
    14281 atcgcccgcg atgtccagcg tgccgccctg aatattcagc tctccgctcc ccgttaacga
    14341 accggttgaa gtgcccccgt tggtaaccgt cagactgccg ccatttaagc tcagcagtga
    14401 atcggcgctg ccattgagcg tgcccacggt ctggctgtga ccattcatat ccagttcagt
    14461 ctctgccgcc agacgcagtt catgggtatt acccaacacg ttatcgttat tcaacaaaag
    14521 cgtcccactg cgcagatcgg taacaccggt atagtcgttg tctttgttag aaagcgatac
    14581 ggtctgaccc ttctggctgc tgaatgccag gtcaccactg ccggtaatct ttgcgctgag
    14641 atcggcggca ttctcgcttt taccgttaga gctcagcacc agagcatcgc tgtcggtagc
    14701 gtgtaaatca agctgggtca ggccgtaacc gatatacaga ccgtcattgt ttaccccact
    14761 cgtcagacga tagtcgtaag tgccgacggc cgctttttgc ccattctgct gaatatcacg
    14821 ttgggcactg ctggagagga tttgcccatt tttatcctgc aattgcagat tgcccgcatc
    14881 gcccagaact tccgcacctt gcgcatcaac cagcttaatg gcgctgttac catcatcgac
    14941 ttcagtgagc gataacgcag agtcaatatc acgagatact gagcggacaa cgtcactgtc
    15001 agaaacctga atcacaccct cgccgcgcaa atccagcgtt ttactaacgt tgaccgtccc
    15061 ttctgtcatc tgtgcacctg ctgtcagggc accaaaatca acggttccgc cggagaatcc
    15121 caaagtgcta atggagtgag cgcccttacc cactgttacc aggcttccct gacccgcagt
    15181 gatcccgcct ctctgcagaa actcagcatt cttttccagg ttaaatgtcg tgttctggaa
    15241 cgccagtgaa ccctggaacc agtctgtata agcaaactca tcgaaggaga attcgtgatt
    15301 ccccatgttt atttcaacaa tacctctacc ttgaagatcg ttatcaaata cccaatcttt
    15361 attggcattg atttgcagtt tgccatcaac atccaggtcc gcacggccga tgttcttgtc
    15421 ttcagaaata accgcgacag caccagaagc gacatcaata tatccgctga atgcatagtt
    15481 attgccggtc agcgccactg tcccactgtc gatacttatt gtaccgttgc cggaaatctt
    15541 attggcaaag gtcatgctat cgtcagcctt caggtgtaat ttcccgcggt tgttgatttc
    15601 tgcggtacct atattttggg tgatccctaa agcgccattt tgctcaattt tgactttacc
    15661 gctaaaggcg gagttatccc cggttaagta agtcgtacca ttgagcgcat tgatatagcc
    15721 caggccctcg ctgtcactga caatatccgc ggaaatagtc gcgtttttat ccgtgtggtt
    15781 taaggtgatt ttcttctcgg atgtctttgc ccacacaaac tcaatttttt cggcatcaat
    15841 aatccctgca gccttcgctg cgcttccttc ctgtgcacct aacgctaact cagagttggt
    15901 gcttaaacta atagtcggcg cagagatttt accaccttcc gaaacagcaa gtgttgtatc
    15961 ggagttttga tcataataac ccaggctaat tttctcactg gcattaactt caccgccgtt
    16021 attaactgac aggtactgat taccaccgta actgccgtaa acgcccataa agaaggtttt
    16081 agaatttacc tggctgcctt caccactgac atttccctgc aaataggtca gacggctggt
    16141 gcccttaaca tggattttgc cgccgttgga gacattaaaa tagccgcgat cgtgtctcat
    16201 gcctgagttt gactcataca aatcatcagc atataactcg ctgccagcat tggagacatt
    16261 caggaacccc
//
LOCUS       DAESHI010000091        16250 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.092, whole genome shotgun sequence.
ACCESSION   DAESHI010000091 DAESHI010000000
VERSION     DAESHI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16250
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.092"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..578
                     /locus_tag="KW011_003056"
     CDS             <1..578
                     /locus_tag="KW011_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HBH7661335.1"
                     /translation="GRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCGDSSDVDND
                     GDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADVPKNMTQQNCQSKYENCYYDNV
                     EQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKES
                     YSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            581..1744
                     /locus_tag="KW011_003057"
     CDS             581..1744
                     /locus_tag="KW011_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBH7661336.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1828..3453
                     /locus_tag="KW011_003058"
     CDS             1828..3453
                     /locus_tag="KW011_003058"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HBH7661337.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="KW011_003059"
     CDS             complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="KW011_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HBH7661338.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="KW011_003060"
     CDS             complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="KW011_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HBH7661339.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4505..5254
                     /gene="yjfP"
                     /locus_tag="KW011_003061"
     CDS             4505..5254
                     /gene="yjfP"
                     /locus_tag="KW011_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBH7661340.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="KW011_003062"
     CDS             complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="KW011_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HBH7661341.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="KW011_003063"
     CDS             complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="KW011_003063"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBH7661342.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7533..8931
                     /gene="ulaA"
                     /locus_tag="KW011_003064"
                     /pseudo
     CDS             7533..8931
                     /gene="ulaA"
                     /locus_tag="KW011_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            8947..9252
                     /gene="ulaB"
                     /locus_tag="KW011_003065"
     CDS             8947..9252
                     /gene="ulaB"
                     /locus_tag="KW011_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HBH7661343.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9262..9723
                     /gene="ulaC"
                     /locus_tag="KW011_003066"
     CDS             9262..9723
                     /gene="ulaC"
                     /locus_tag="KW011_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HBH7661344.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVADVVEPRYYQ
                     AILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDILI
                     TMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            9737..10387
                     /gene="ulaD"
                     /locus_tag="KW011_003067"
     CDS             9737..10387
                     /gene="ulaD"
                     /locus_tag="KW011_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBH7661345.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10397..11251
                     /gene="ulaE"
                     /locus_tag="KW011_003068"
     CDS             10397..11251
                     /gene="ulaE"
                     /locus_tag="KW011_003068"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HBH7661346.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            11251..11937
                     /gene="ulaF"
                     /locus_tag="KW011_003069"
     CDS             11251..11937
                     /gene="ulaF"
                     /locus_tag="KW011_003069"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HBH7661347.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            12034..12585
                     /locus_tag="KW011_003070"
     CDS             12034..12585
                     /locus_tag="KW011_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HBH7661348.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(12660..12935)
                     /locus_tag="KW011_003071"
     CDS             complement(12660..12935)
                     /locus_tag="KW011_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBH7661349.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            13262..13657
                     /gene="rpsF"
                     /locus_tag="KW011_003072"
     CDS             13262..13657
                     /gene="rpsF"
                     /locus_tag="KW011_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBH7661350.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13664..13978
                     /gene="priB"
                     /locus_tag="KW011_003073"
     CDS             13664..13978
                     /gene="priB"
                     /locus_tag="KW011_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HBH7661351.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13983..14210
                     /gene="rpsR"
                     /locus_tag="KW011_003074"
     CDS             13983..14210
                     /gene="rpsR"
                     /locus_tag="KW011_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBH7661352.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14252..14701
                     /gene="rplI"
                     /locus_tag="KW011_003075"
     CDS             14252..14701
                     /gene="rplI"
                     /locus_tag="KW011_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBH7661353.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14813..15784)
                     /locus_tag="KW011_003076"
     CDS             complement(14813..15784)
                     /locus_tag="KW011_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBH7661354.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(15837..>16250)
                     /locus_tag="KW011_003077"
     CDS             complement(15837..>16250)
                     /locus_tag="KW011_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBH7661355.1"
                     /translation="WAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIGTFMDMT
                     PAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVSKLPINK
                     IIKPMLPFYAVMVLVLAMVTYIPQISMALPKALGY"
BASE COUNT         3817 a         3968 c         4322 g         4143 t
ORIGIN      
        1 ttggtagacc gaataatcct tttgaaccgc gtcgcaatcg ctacgcacaa aaatacttaa
       61 cgttggcgct aatgggcggt gccgcttttt tcgtattgaa aggttgtggc gatagtagcg
      121 atgtcgataa cgacggcgac ggaacgtttt acgcgacagt gcaggattgt attgatgacg
      181 gtaataatgc cgacatttgc gcgcgtggct ggaacaacgc caaaacggca ttttatgccg
      241 atgttccgaa gaatatgact cagcagaact gtcagtctaa gtacgaaaat tgctactacg
      301 acaatgttga acagagttgg atcccggtcg tttctggatt tttgttaagc cgggttattc
      361 gtaaagatcg cgatgagccg ttcgtctata acagcggcgg atcctcattt gcttcccgcc
      421 ccgtctggcg caacacttcc ggtgattact cctggcgctc cggttctggc aaaaaagagt
      481 cttactcttc aggcggcttt accaccaaaa aagcgtcaac cgtttcgcgc ggcggctatg
      541 gtcgttcttc cagcgcccgt gggcattggg gaggctaatc atgctgagac acaacgttcc
      601 tgtgcggcgg gatctggacc aaattgccgc tgacaacggt ttcgactttc atatcatcga
      661 caatgaaatc tattgggacg agagtcgggc ttaccgtttt actctgcgcc agattgaaga
      721 gcagatcgaa aaaccaactg cggaactgca tcagatgtgc cttgaggtgg tggatcgcgc
      781 ggttaaagac gaagagatcc tgacgcaact ggcgatcccg ccgttgtact gggatgtgat
      841 cgccgaaagc tggcacgcca gcgatccttc gctgtatggc cggatggatt ttgcctggtg
      901 tggcaatgcg ccggtgaagc tgctggagta caacgccgat acgccgactt cgttgtatga
      961 gtcggcttat ttccagtggt tgtggctgga ggatgcccgg cgcagcggca ttattccgcg
     1021 tgatgccgat cagtacaatg ccattcagga acgcctgatt tcacgcttta gcgagcttta
     1081 tagccgggag ccgttttatt tctgctgctg tcaggacacc gatgaagaca ggactaccgt
     1141 gctgtacttg caggactgcg cccagcaggc agggcaggag tcgcggttta tctacattga
     1201 agatctcggt ttgggcgtcg gcggtgtact gaccgatctt gatgataatg tcatccagcg
     1261 tgcatttaag ctgtatccgc tggagtggat gatgcgtgac gataacggtc cgctgctgtg
     1321 caagcgtcgc gagcaatggg tggagccgtt atggaaaagt atcttgagta ataaagggct
     1381 aatgccgctg ctttggcgct tcttccctgg tcatcctaac cttcttgcat cctggtttga
     1441 gggtgaaaaa tcgcagattg ccgctggcga aagctatgtg cgtaaaccga tctactcgcg
     1501 cgaaggcgga aacgtcacca ttttcgacgg tcagaataac gtcgttgacc acgctgatgg
     1561 tgattacgcc gatgaaccga tgatctacca ggcgtttcaa cctctgccgc gatttggcga
     1621 tagctacaca ctcatcgata gctggattgt cgatgatgaa gcgtgcggaa tggggatccg
     1681 tgaagataac acactgatca ccaaagacac ctcacgtttc gttccgcatt acattgctgg
     1741 ataagaatgt tttagcaatc tctttctgtc atgaatccat ggcagtggcc atactaatgg
     1801 tgactgccat tgatggaggg agacacagtg cactggcaaa ctcacaccgt ttttaatcaa
     1861 cctataccat taaataacag caacttatac ctgtctgatg gcgcgctttg cgaagcggta
     1921 acgcgtgaag gtgctggctg ggatagcgat tttctagcca gtattggtca gcagttagga
     1981 acggctgaat cccttgaact ggggcggctg gcgaatgtaa acccgcctga actgttgcgc
     2041 tacgatgccc aggggcggcg tctggacgat gttcgtttcc atcccgcatg gcacctgctg
     2101 atgcaggtgt tatgtaccaa tcgggtgcac aatcttgcct gggaagaaga cgctcgctcc
     2161 ggcgcatttg tggcgcgcgc ggcgcgtttt atgttacatg cgcaggttga ggctgggtcg
     2221 ttatgtccaa taacgatgac ctttgccgcc acgccattgt tgttacagat gttacccacg
     2281 ccgtttcagg actggaccac gccgctgctg agcgatcgct acgattctca cttattgcca
     2341 ggtgggcaaa aacgcggttt gttgattggc atgggaatga cggaaaagca gggtggttcc
     2401 gatgtcatga gcaacaccac ccgtgcagag cgtttggaag atggctctta tcggctggtg
     2461 gggcataaat ggtttttctc ggttccgcaa agcgatgcgc atctggtgct ggcgcagacc
     2521 acgggcggtc tgtcctgttt ttttgtgccg cgctttttgc ctgacgggca acgcaacgcg
     2581 attcgcctcg aacggctgaa agataagctg ggtaatcgct ctaacgccag ttgtgaagtg
     2641 gagtttcagg atgccattgg ctggttattg gggcaggaag gggaaggaat tcgtctgatc
     2701 ctgaaaatgg gggggatgac gcgttttgat tgcgccctgg gtagccatgc catgatgcgc
     2761 cgtgcgtttt cgctggcgat ttatcatgca catcaacgcc atgtttttgg taatccattg
     2821 atccaacagc cccttatgcg tcatgtctta agtcgcatgg cacttcagct tgaagggcaa
     2881 acggcgttgc tgtttcgtct tgcgcgagcg tgggaccggc gcgccgatgc caaagaagcc
     2941 ctgtgggcgc gtttatttac gcctgcggca aaatttgtga tctgcaaacg aggtatgccg
     3001 tttgtggccg aagcgatgga ggtgctgggc ggcattggtt attgcgagga gagcgagctg
     3061 ccgcggcttt accgggagat gccggtaaac agtatttggg aaggttccgg caatattatg
     3121 tgcctggatg tgttgcgcgt tctcaataag caagcgggcg tatacgactt attgtcggaa
     3181 gcatttgtgg aagtgaaagg gcaggatcgc tattttgatc gcgcggttcg tcgtttacag
     3241 cagcagctgc gtaagccagc tgaagaactg gggcgagaga ttactcatca gctattcctg
     3301 ctgggctgcg gtgcgcaaat gttgaaatat gcttctccgc caatggcgca ggcgtggtgt
     3361 caggtgatgt tagatacgcg cggcggcgta cggttgtcag agcagatcca gaatgattta
     3421 ttgctgcggg cgacgggggg agtgtgtttg taaacgtata cgactgatgc gacgctggtt
     3481 tcgattaact caatgaaata tgtgaaaatt gtaggccgga caaggcgctc gcgccgcatc
     3541 cggcattgtt catttgctgt agatgacact catgcataca gtatcgcctg tacgcgccag
     3601 ttatcaggct gctggtcttc gtacatggta ataatgcggt accatgacgc accttgttca
     3661 tcggccttta acgccacgat acgttccacg tcctgcggat cccctgaaat attgctaact
     3721 gagatctgac ctatttcatt caggcccgtc acgcagtcag cactggcgaa ttctgcagac
     3781 tgtgccgtgg cgctcgcagg ataagtcact aattgcagcg agggtgaggc aagtaattgt
     3841 ttcatcgtca gctccatttt tcgtttcccc acatgttgca gggcattcca tcgcggtaat
     3901 aaacgcccag gcttccagcc ggtaatttat tgtgcacagg taaacgcata gggacgcaaa
     3961 gcaatgtaaa gctgactatg taagttgtct gatttattta cggtacaaaa tggcttgtga
     4021 ataccactgt cctgtcacga tggtttcgtc caccatgacg acaacgtaat aatcagcttt
     4081 tgcagcgaca gcttttgctc tgatttctgc taatgcgtca tccggagaac cccgaaccat
     4141 cgtgcttacg ctacctattc gctgtaaccc ttgcgtctgg tcgcggcgaa tctcttgcgg
     4201 atggtccgtt actggcggtg ctggctgtgg cgtaccttgc agtgcgctac aggcacttaa
     4261 catcaacacc aataataaac tggcaaaccg gtaaataacg ctattacgtt tcctgctaac
     4321 catagtgtag tgccttatta atttaacttt gggggaatgt tcccgaattg ttacctggta
     4381 cgcgattttc gaatgaaaac gtgacgaaag cgtaacccac atctcaacat tatgaactaa
     4441 taccgccagc agaaacttgt gctaaggatt tcttcatgtc ataccagaca aaaaggagag
     4501 acagatgatt gaaatagaat cacgcgagct ggcagatatt cccgttcttc atgcttatcc
     4561 tgtcgggcaa aaagataccc cgttaccgtg cgtaattttt tatcacggct ttacttcatc
     4621 cagcctggtg tatagctatt ttgccgttgc gctggcgcag gctggtttgc gggtgatcat
     4681 gccggatgcg cccgatcacg gtagccgttt tagtggcgac gaagcgcggc ggttaaatca
     4741 attctggcaa atcttgctac aaagtatgca ggaattcact actttacgtg cggcaatagc
     4801 cgaagaaaac tggctgcttg atgaccgtct ggcagtcggt ggcgcgtcga tgggcgcgat
     4861 gacggcactg ggaattaccg ctcgccaccc cacggtgaaa tgtaccgcca gcatgatggg
     4921 atcgggctat tttacatcgc tcgcccgttc actgtttcca ccgctgatac ctgaaacggc
     4981 agcacagcag aatgaattca ataacattat cgcgccgctg gcagagtggg aagcgacaaa
     5041 ccacctggaa caacttgggg acagacctct actgctgtgg catggcctcg acgatgacgt
     5101 tgtgcctgcc gacgaatcac tacgtttgca gcaggcctta agcgagacgg gacgggataa
     5161 actgctaacc tgttcatggc agccaggcgt gcgtcaccgc attacgcctg aggcgttaga
     5221 tgctgccgtg acatttttcc gccagcatct ttaaacacgc agaatgctga ccccttgcgc
     5281 ttccagttgt tgcaggattt ccgggttagc atttttgccg gtgataagca tatcgatttg
     5341 atcggcacgg ctaaaaagca ttcccgcgcg ttcgccaatc ttactgctat caaccagtac
     5401 caccagtttc cctaccacgc tcagcatctt ctgctctgcc attgctgtca gcatatcggt
     5461 tttatacaac ccttctgcgg tcagcccttt tccgctggta aacatccagt gcccggcata
     5521 gagactgttt tcgctgccct gcgggcttaa agtgatggac tgacttttgt tgtactgtcc
     5581 acccataatg atcacgctgt catgttcttg atcgatcagg taatttgcca gcggtagata
     5641 attagtgatg atttgcactg gcttgccaca catttcccgc ccaagcagaa acgcggtgga
     5701 gccgcagttg atgactacgc tttcgcccgg attaaccagc tgcgaggccg ctttagcaat
     5761 acgtactttt tcatcgtgat tctgcgcctg atgcagattc atcggcgtcc agcgcgggcg
     5821 ctgttgggta atagcttctg cgccattgcg cacctttttc agtttgccgc gttcgtcaag
     5881 tttattgata tcgcgtcgcg cagtggcagg cgaaattccc agacgctcaa cgactttctc
     5941 aacggtcaca aagcccaatt gtgcgagcat ttccaggagg atttgatgtc tttgtgcttc
     6001 agtcatgagc tattccgata aaatttgatt tttttagatg atatttgaaa tagctaggaa
     6061 atactacgcc ggatagcgcg aacttctcca cgctatccgg tataaacgac agattacagg
     6121 aatgacttga acggcagatc cggttcaata gtgaagcaat catcgaaacc gcgcggatag
     6181 tggtactcga agttgtcttt atccagcggc caggtaaatt tgccgccaac ctgccagata
     6241 aacggcttga agccatactt cagacgatct tttttcatct cccacagcac gcggatctct
     6301 tgcggatcgg cctggaagtt tgaccagatg tcgtggtgga acgggataac cactttcgca
     6361 ttcagggctt cacccatacg cagcatatcg gcgctggtca ttttgtcggt aataccgcgc
     6421 gggttctcac catacgatcc taacgccaca tcgatctgat gttcgttacc gtgcttcgca
     6481 taatagttag agtagtggga atcgccactg tgatataggg aaccgccagg ggttttgaac
     6541 aggtagttta ctgcgcgatc gtccatgcca tctggcagta cgccagccgc tttttgatcg
     6601 gcaggcaggg tgatcagtgc agtacggtcg aaagcgtcaa gcgcatgaat ttcaatgtct
     6661 ttcactttta caacatcgcc cggtttgacc acgatgcaac gctctttcgg tacgccccag
     6721 ccaatccaca aatcaacgca ggttttcggt ccaatgaatg gtacgtcatc agcacagttc
     6781 tgcatcacgg cagcagcgac gttaacgtcg atatgatcgt tgtgatcgtg agtcgccagt
     6841 accgcgtcga tctggcgaat cgcaaacgga tcaagaacaa acggggtggt gcgtaggttt
     6901 ggctgcagtt ttttcacgcc agccatgcgc tgcatctggt gaccctgttt cattaacggg
     6961 ttaccgtgac tttgtttgcc agtgccgcac cagaaatcaa cgcaaacgtt ggtgccacct
     7021 tctgatttca accagatccc ggtgcagcct agccaccaca tcgcaaatgt gccaggggcg
     7081 acctgctctt gttcaatttc ttcattcagc cagctacccc actccgggaa agtgctcagg
     7141 atccaggatt ctcgggtgat acttttcact ttactcatcg ccatttacct tcatgatagt
     7201 tcaattcgaa tcaatatgtg attggttttg attaatcctg acactatttt ttcaggaagg
     7261 caatgaccat tttttgactt tttgccaggg aagttgttgt tgattttgag tgtggaaaga
     7321 tttaatggaa tgtgtagtta aattaactaa atgaatttaa ataaataatt gtttcgttgt
     7381 gtgaatccca ctctatccgt gtggaattat ttgcgggtcg cgtcacattt aatcataaat
     7441 aatcttgttg tgattacttt tgaaaattag agtgagtgca caacattccg ggtgtgcgga
     7501 atacccggtt acctcttctt caggagatcg ttatggagat cctctacaac atctttaccg
     7561 tgttttttaa ccaggtcatg accaatgccc cgttgttgct gggtattgtg acctgtctgg
     7621 gctacatcct actgcgcaaa agtgtcagcg ttattattaa aggcacgatt aaaaccataa
     7681 ttggtttcat gttgttgcag gcagggtccg gcatcctcac cagcaccttc aaaccggtgg
     7741 tggcgaaaat gtccgaagtc tacggcatta aacggcgcaa tttccgatac ttacgcgtcg
     7801 atgatggcaa ccatcgaccg catgggcgat gcctatagct gggtaggtta cgcggtgttg
     7861 ttagcgctgg cgctgaacat ctgttacgtg ctgctgcgtc gcattaccgg tattcgtacc
     7921 atcatgctga ctggtcatat catgttccag caggcggggc tgattgccgt tacgttgttt
     7981 attttcggct actccatgtg gacaaccatt atctgcacgg cgattctggt ttcgctctac
     8041 tggggcatta cctccaacat gatgtacaag ccgactcagg aagtgacgga cggctgcggt
     8101 ttctccatcg gtcaccagca acagtttgca tcatggactg cctataaagt tgcgccgttc
     8161 ctcggcaaaa aagaggaaag cgttgaagac ctcaaactgc caggctggct gaatattttc
     8221 cacgacaaca tcgtctccac ggcgattgtc atgaccatct tctttggtgc cattctgctc
     8281 tccttcggta tcgacaccgt gcaggcgatg gcaggcaaag tgcactggac ggtctatatc
     8341 ctgcaaaccg gtttttcctt cgcggtggcg atcttcatca tcactcaggg tgtgcgcatg
     8401 tttgtggcgg aactctctga agcgtttaac ggtatctccc agcgcctgat ccctggtgcg
     8461 gttctggcga ttgactgtgc ggctatttat agcttcgcgc cgaacgccgt ggtctggggc
     8521 tttatgtggg gcaccatcgg tcagctgatt gcggttggca tcctggtcgc ctgcggttcc
     8581 tcgatcctga ttatcccagg ctttatcccg atgttcttct ccaacgccac catcggcgtg
     8641 ttcgctaacc acttcggcgg ctggcgtgcg gcgctgaaga tttgtctggt gatggggatg
     8701 atcgaaatct ttggttgcgt ctgggcggtg aaacttaccg gtatgagtgc ctggatgggg
     8761 atggcggact ggtcgattct ggcaccgccg atgatgcagg gcttcttctc catcggtatc
     8821 gcctttatgg ccgtcatcat tgtaattgca ctggcttata tgttcttcgc tggccgcgca
     8881 ctgcgcgcag aagaagatgc agaaaaacaa ctggcagaac agtctgctta ataaggagtt
     8941 ttgattatga ccgtacgtat tctggctgtg tgtggcaacg gacaaggcag ttccatgatc
     9001 atgaggatga aagtggacca gtttttaacc caatcaaaca ttgaccacac ggtaaatagc
     9061 tgcgcggttg gcgaatacaa aagcgagttg agcggcgcgg atatcatcat cgcttctacg
     9121 cacattgcgg gcgaaatcac cgtgaccggc aacaaatacg tggttggcgt gcgcaacatg
     9181 ctctctcctg ccgactttgg cccgaaactg ctggaagtga tcaaagcgca tttcccgcag
     9241 gatgtgaagt aaggacgcgc catgaaatta cgtgattcgc tggcggagaa taaatccatc
     9301 cgcctgcagg ctgaagctga gacatggcag gacgcagtaa aaatcggcgt tgacctgctg
     9361 gtggcggatg ttgtcgagcc gcgttactac caggcgattc tggatgcggt tgaacagcac
     9421 ggtccgtact ttgtgctcgc accaggcctg gcaatgccgc acggtcgccc ggaagagggc
     9481 gttaagaaaa ccggttttgc gctggtgacg ctgaaaaagc cgctggagtt caaccacgaa
     9541 gataacgacc cggtggatat cctcatcacc atggcggcgg tcgatgccaa cacgcaccag
     9601 gaagtgggca tcatgcagat cgtcaacctg tttgaagatg aagaaaattt cgaccgttta
     9661 cgtgcctgcc gtaccgagca ggaagtactg gatctcattg accgcaccaa cgcggcagct
     9721 taagaaggaa ttgaagatgt cattaccgat gttgcaagtc gcgctggaca accagactat
     9781 ggatagcgcc tacgaaacca ctcgcctgat tgccgaagaa gtcgacatta tcgaagtggg
     9841 taccattctg tgcgtgggcg aaggcgtgcg tgcggttcgc gatctgaaag cgctctaccc
     9901 gcacaaaatc gtgctggcag acgccaaaat tgccgatgcg ggcaaaattc tttctcgtat
     9961 gtgcttcgaa gccaacgctg actgggtgac cgtgatttgt tgtgcggata tcaacaccgc
    10021 caaaggtgcg ctggacgtag caaaagagtt taacggcgac gtgcagatcg aactgaccgg
    10081 ttactggacc tgggaacagg cgcaacagtg gcgcgacgca ggtattcagc aggtggttta
    10141 tcaccgcagc cgtgacgcgc aggccgctgg cgtggcgtgg ggtgaagccg atatcactgc
    10201 gatcaaacgt ctttccgata tgggcttcaa agtcaccgtc accggtggcc tggcgctgga
    10261 agatctgccg ctgttcaaag gcattccaat tcatgtgttt atcgccggtc gcagcatccg
    10321 tgatgccgct tctccggttg aagccgcgcg tcagttcaaa cgttccatcg ccgaactgtg
    10381 gggctaagga gcggatatgt tgtccaaaca aatcccgctt ggcatctatg aaaaagcgct
    10441 ccccgccggg gagtgctggc tggaacgcct gcgactggca aaaacgttgg gcttcgattt
    10501 tgtcgagatg tcggtagatg aaactgacga gcgcctgtcg cgcctcgact ggagccgcga
    10561 gcagcgtctg gcgctggtga atgcgattgt tgaaaccggc gtgcgtgtgc cgtccatgtg
    10621 cctttctgcc catcgtcgtt tcccgttggg cagcgaagat gacgcggtgc gggcgcaggg
    10681 gctggagatt atgcgtaaag ccatccagtt cgcccaggac gttggcattc gcgtgatcca
    10741 gctggcgggc tatgacgttt actatcagga agccaataac gaaacgcgtc gtcgtttccg
    10801 tgacggcctg aaagagagcg ttgagatggc gagccgcgcg caggtgacgc tggcgatgga
    10861 gatcatggat tatccgttga tgaactccat cagcaaggcg ctgggttacg cgcactatct
    10921 caacaacccg tggttccagc tctacccgga tatcggcaac ctgtcggcgt gggacaacga
    10981 tgtgcagatg gagttgcagg ccggaatcgg gcatatcgtc gcggtgcatg tgaaagacac
    11041 caaaccgggc gtcttcaaaa acgtgccatt tggcgaaggt gtggtggatt tcgaacgttg
    11101 cttcgaaacg ctcaaacaga gtggctattg cgggccgtac ctgattgaga tgtggagcga
    11161 aacggcggaa gacccggcgg cagaagtggt gaaagcgcgt gattgggtga aagcgcgcat
    11221 ggccaaagca ggcacggtgg aggcggcata atgcaaaagc taaaacagca ggtgtttgaa
    11281 gccaacatgg atctgccgcg ctatgggctg gtgaccttta cctggggcaa cgtcagcgct
    11341 atcgaccgcg aacgcgggct ggtggtgatc aagcccagcg gcgttgccta cgaaaccatg
    11401 aaagcagacg atatggtggt ggtcgatatg agcggcaagg tggtggaagg ggagtatcgc
    11461 ccgtcttccg acaccgcgac gcatctcgaa ctctaccgtc gttacccgtc gcttggcggc
    11521 attgtccata cccactccac tcatgctact gcatgggcgc aggcggggct ggcgatcccg
    11581 gcgttaggca ctacgcacgc cgactacttc tttggcgaca tcccgtgtac gcgcggatta
    11641 agcgaagaag aggtgcaggg cgagtatgaa ctgaacaccg gcaaagtgat tatcgaaacg
    11701 ctgggtgacg ccgagccgct gcatacgccg gggattgtgg tgtatcagca cggtccgttc
    11761 gcctggggga aagatgccca cgacgcggtg cataacgcgg tggtgatgga agaagtggcg
    11821 aaaatggcgt ggattgcgcg cagtattaac ccacaactga atcacatcga cagcttcctc
    11881 atgaataaac acttcatgcg taagcacggt cctaacgcct attacgggca gaagtaagca
    11941 gtgcgccccg gaattattac ctcctgcggg atgtaattgg tctgattccg gggccgcttc
    12001 agttaaaaaa tcgtataaac aacaggtgtt tttatggcta tcaatggatt gtttgtcaga
    12061 acggatccgc aacgtcgccg ctatggcgtt gcgttacttg tgggtgtact ttcaggaatt
    12121 atttcagcat ttgttaaatg gggagcggaa gtccccttgc caccgcgcac attttccggt
    12181 ggtcgcgatg aatttaaccc gccgtatatc tttctacggg attatttagg tatcgaccca
    12241 acccaaactg tgtatacatt ctcggaacat attatcaacc cggtgatggt gacgcatatc
    12301 atcttctcac tggtttttgc gatagggtat tgtattgtcg cggaaatttt cccgaaagtg
    12361 aaattatggc aggggatatt agcagggctt attgtgacag tggcagtcca cggtatattt
    12421 tgcccggcgc tgaatttaac gccgccgctc actcaattgc cttttgacga atatgcctct
    12481 gaaatcctgg ggcatatttt ctggttctgg ataatagaaa ttatgcgccg cgacttacgt
    12541 aatcgcatta ctcatgagcc agatccggaa gtcgcaatcc ggtgaataac cgcataaaaa
    12601 agccccctgc gaagggggca aagcaaacta tggcaatgtt tcgttggtta tacctggtgc
    12661 tagcgataaa tatccgcgct ggcgtgcata ttgccgttac tccccggttc ccgcatcaga
    12721 attacgtggt agtacgttgc gccctgcgca tctgtctctt catttaatgc ctgacgcgct
    12781 tcgctttcag tggcgaaatt atgattgata taaatcacgc ctaagctttg cacatcatcc
    12841 atgtttctgg cctggtggtt attaatttca atggctgccc atgtatttgc acttagcaaa
    12901 agcacagcca gaagggctaa aactcgactg aacatagata cctcctcgac ggctgacttt
    12961 gtgtgctctc ctctgtgatg atcttctgat ttaattttaa tcaatgataa agaagttgat
    13021 ggtgaccatt tctgatgcag ttgttcaaaa aaacaccatg atgaagtgtg atgaactcca
    13081 aatcagcggt ttagaggtta attgcgaaag gggagattta tttcggctct gcccttgagt
    13141 ttagcggggc atacaagtac tataacgcgt catttttcag ccgaccttta acacgttcct
    13201 tgcctccccg ggattcggct gacccagaca ggaggctgaa taatccgtaa ggagcaattc
    13261 gatgcgtcat tacgaaatcg tttttatggt ccatcctgac cagagcgaac aggttccggg
    13321 catgatcgag cgctacactg ctgccatcac tggtgcagaa ggcaagatcc accgtctgga
    13381 agactggggc cgccgtcagc tggcttaccc gatcaacaaa ctgcacaaag cacactacgt
    13441 tctgatgaac gttgaagctc cgcaggaagt gatcgatgag ctggaaacta ccttccgctt
    13501 caacgatgcc gttatccgca gcatggttat gcgtaccaag cacgctgtta ccgaagcatc
    13561 tccgatggtt aaagcgaaag acgagcgccg tgagcgtcgc gatgatttcg caaacgaaac
    13621 cgctgatgat gctgaagctg gggattctga agagtaattt ctgatgacca accgtctggt
    13681 gttgtccggc accgtgtgca ggactcccct tcgaaaggtc agtccatcag gaattcctca
    13741 ctgccagttc gtgcttgagc atcgttctgt gcaggaggaa gccggttttc accggcaggc
    13801 gtggtgccaa atgcccgtta ttgttagcgg acacgaaaac caggccatta ctcacagtat
    13861 aacggtcggc agtcgcataa ccgttcaggg gttcatttca tgccacaagg caaagaacgg
    13921 actgagcaaa atggttttgc atgccgagca gattgaattg atagattctg gagactagcc
    13981 atatggcacg ttatttccgt cgtcgcaagt tctgccgttt caccgcggaa ggcgttcaag
    14041 agatcgacta taaagatatc gctacgctga aaaactacat caccgaaagc ggtaagattg
    14101 tcccaagccg tatcaccggt acccgtgcaa aataccagcg tcagctggct cgcgctatca
    14161 aacgcgctcg ctacctgtcc ctgctgccgt acactgatcg ccatcagtaa tcggtcacgg
    14221 tccattaata cgactttgag aggataaggt aatgcaagtt attctgcttg ataaagtagc
    14281 aaacctgggt agcctgggtg atcaggtaaa cgttaaagcg ggctatgctc gtaacttcct
    14341 ggtaccgcag ggtaaagctg ttccagctac caagaaaaac attgaattct tcgaagcacg
    14401 tcgcgctgaa ctggaagcta aactggctga agttctggca gctgctaatg ctcgcgctga
    14461 gaaaatcaat gcactggaaa ctgttaccat cgcgtctaaa gctggcgacg aaggtaaact
    14521 gttcggttcc atcggtactc gcgacatcgc tgacgctgta actgcagctg gcgttgaagt
    14581 ggctaagagc gaagttcgtc tgccgaacgg cgttctgcgt accactggcg agcacgaagt
    14641 gagcttccag gttcacagcg aagtattcgc gaaagtgatc gtaaacgtag tagctgaata
    14701 attcgttatt caacgagacg taaaaacgcc gaccagtggt cggcgttttg cttttgtagc
    14761 gagcacttgc agatatcgat aagggccaca ataagcagcc ctacgaagat aattacttag
    14821 ctgcctgaat atcagccagc attggggcaa gggctgggtt gtccttagcg aagtttgcgt
    14881 acattggctg tactttctcg ataaacggag ctttatctac ctggtagact ttcacgccca
    14941 ttttatccag ttttgcaaat tcttgtttct cttcctcgct ccacaagtct ttcatgtagc
    15001 tcattgattc tttagctgct tttaatataa ctgcctgatt ttccgggcta agtttatcaa
    15061 acgcagcatt gctcatcaga accacatcgg gaaccatagt gtgttcgtca aggctgaagg
    15121 attttgccac ttcaccgtga cgcatggtgg tcagcgccat cacgctgttt tctgccatat
    15181 cgaccacacc ctgctgtaag gctgtataca actcgccgta atccagtggc gtcgggttac
    15241 cgcccatgac tttgatcatt tcaactgcgc cagggcttgg ctggacacgg actttcatgc
    15301 ctttcagatc gtctggtttc agtactggtt tattgccata gaaactgcgg gcgcctccat
    15361 cataaaaagt cagcccgaaa taacctttgc ttttcgttga gtcgaggatt tttctcccga
    15421 tatcgccctg catgacctga taataatgat cacgatcgcg gaacaaataa ggcaggctaa
    15481 ataatttata ggaattttca aatgcttcca tttctgccgc attggttttt accagtggaa
    15541 tagcgccaga acgaatcagc tccattgttt ctcgttgagt acccaatgtt ccatttggat
    15601 aaatacgaat agtaatatca ccgttagagt actctttgtt ctttttcgca aagaactcca
    15661 tcgctttatg aacaggatga gacttatcct gattatgact cagttttaat gtcactgccc
    15721 aggactggct tgcggataac ataagaacag caccgataat gccagcggat acttttattt
    15781 tcatagatgt tccttttcta ataaatatgg cggcaggtgc cgccagtgaa tgcgcatcag
    15841 tagcccagtg cttttggcaa tgccatactg atttgcggaa tataggtcac cattgccagc
    15901 accaatacca tcacggcata gaatggcagc ataggtttga taattttatt aatcggtaat
    15961 ttagatacac tacaaccgac aaataacaca ctgcctacag gcggagtaca aataccgata
    16021 cacatgttca gtaccatgac aataccaaag tgaatcgggt ccatgcccag ttcggtgaca
    16081 acaggtaaaa atatcggcgt aaaaatcagt accgccggtg tcatatccat aaaagtgccg
    16141 ataatcaaca ggataatatt gataataagc aggattatta ttgggttgtc agaaatagtg
    16201 ttaagcaaat cgagaatcag gaagggaacg tcagcgtttg acattgccca
//
LOCUS       DAESHI010000092        16216 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.093, whole genome shotgun sequence.
ACCESSION   DAESHI010000092 DAESHI010000000
VERSION     DAESHI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16216
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.093"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..173
                     /locus_tag="KW011_003078"
     CDS             <1..173
                     /locus_tag="KW011_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH7661356.1"
                     /translation="RNRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQRF
                     GMTPGEYRRKFR"
     gene            complement(345..674)
                     /locus_tag="KW011_003079"
     CDS             complement(345..674)
                     /locus_tag="KW011_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HBH7661357.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(679..864)
                     /locus_tag="KW011_003080"
     CDS             complement(679..864)
                     /locus_tag="KW011_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HBH7661358.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(861..3500)
                     /locus_tag="KW011_003081"
     CDS             complement(861..3500)
                     /locus_tag="KW011_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HBH7661359.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3708..4697
                     /gene="ldhA"
                     /locus_tag="KW011_003082"
     CDS             3708..4697
                     /gene="ldhA"
                     /locus_tag="KW011_003082"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBH7661360.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4808..5230
                     /gene="hslJ"
                     /locus_tag="KW011_003083"
     CDS             4808..5230
                     /gene="hslJ"
                     /locus_tag="KW011_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HBH7661361.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5227..5493)
                     /locus_tag="KW011_003084"
     CDS             complement(5227..5493)
                     /locus_tag="KW011_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HBH7661362.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5767..9291
                     /gene="nifJ"
                     /locus_tag="KW011_003085"
     CDS             5767..9291
                     /gene="nifJ"
                     /locus_tag="KW011_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBH7661363.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9657..10790
                     /gene="ompN"
                     /locus_tag="KW011_003086"
     CDS             9657..10790
                     /gene="ompN"
                     /locus_tag="KW011_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HBH7661364.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10931..11365
                     /gene="uspF"
                     /locus_tag="KW011_003087"
     CDS             10931..11365
                     /gene="uspF"
                     /locus_tag="KW011_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HBH7661365.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11951..12865
                     /gene="sitA"
                     /locus_tag="KW011_003088"
     CDS             11951..12865
                     /gene="sitA"
                     /locus_tag="KW011_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HBH7661366.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            12865..13692
                     /gene="sitB"
                     /locus_tag="KW011_003089"
     CDS             12865..13692
                     /gene="sitB"
                     /locus_tag="KW011_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HBH7661367.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            13689..14546
                     /gene="sitC"
                     /locus_tag="KW011_003090"
     CDS             13689..14546
                     /gene="sitC"
                     /locus_tag="KW011_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HBH7661368.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            14543..15400
                     /gene="sitD"
                     /locus_tag="KW011_003091"
     CDS             14543..15400
                     /gene="sitD"
                     /locus_tag="KW011_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HBH7661369.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(15601..>16216)
                     /locus_tag="KW011_003092"
     CDS             complement(15601..>16216)
                     /locus_tag="KW011_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HBH7661370.1"
                     /translation="SLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFDDRD
                     QAVPYTATADDVAPTGQRIWQELQSGKWGEIAPFTVTPEMLEAAREARRQGIEAWRTE
                     QEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIVRDVMTWGDADNQQVKLSMPEL
                     EELAAAMAQAQVERNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
BASE COUNT         4042 a         4104 c         3901 g         4169 t
ORIGIN      
        1 ttcgtaaccg tcgtctcgat ttttgtgcag atgcgattcg ccatgccgca gatgatgaaa
       61 aactggcagg catcggcttt cattggggat tttctgacca gagtcatttt tcaacggtat
      121 ttaagcaacg ctttgggatg acgccaggcg agtatcgacg taaattccgt taactaattc
      181 gcgttgtgtg tactgcaacg ccaattagtt aaaacagata tttgactatg catttttcag
      241 ggcaaaaggc aatttgacag gagtttttga atattcacct tgtttcagga aaatagatca
      301 atcgctttct cctgtagcgt tatcccgata ttgcggtttt gctattaaaa ttttcgcacc
      361 catttaccgt ttatcccttg tacatattcc cccggtttgg cccgagctac gagcttttgc
      421 ccggcgagtt tcgcgatatc atctaccgac acattatttt gctttgccag ttgttggtaa
      481 ctcgctttac gttcggcatt aatgtcggtt actaatttct ctgtttcagc atccgttttc
      541 agcgcaacca gataaccata aaatgtttca cctacccgcc cctgagttct ggcttcatcc
      601 agagtcaatg ccattacatt gctgcttacc agcccaacaa gaaacgcaca aagaagtaat
      661 gttttcttca tcatcgcctc agaaaagatc gctacgagtt tcaagcagct cttcgacatc
      721 tttgtctgcc ttgatgatga tctcatgctc aattttaacg ttcatattga tagtgatcgg
      781 ttccttgggc gcagcgactt caatacgcgg cgtacagcca accagcataa aagatgacgt
      841 caacgcagcc agtaaaattt tcattgtttt tcctcacact cttttccttg cggacagtca
      901 tttcccggca gacgtgcgtt ctgctccaac catgcctgga gattatcgcc aaagcgtaaa
      961 ctgcgccaca gcgtaaaaat attctcttca tgatggtaat taaggtttac cgtaccgctt
     1021 ttaccatcaa cgcgactggt acctgtaatg ttggcctgca tggttaataa accgagatta
     1081 tctaagttaa tttttgtcga tgaccggcta atttccatat agcgcaacca gttaattgct
     1141 gaacccgcag tcatattgtc tttcaccacc gcatctgcgg tgtctttatc cagccgcaag
     1201 gtcattggcc cgctatttgc cagccaacca tctttcacta tccatttttc gttgtttagc
     1261 cataacggca atgcaccact aaatgccccg gacatggcga attgtttcgg attgacggcg
     1321 ctaaccagtt cgctggatga gagattattc agccgcaaca gcgccgggtc atgttgcggc
     1381 atacgtaatt gtttcatcag tacgttaccg cctaacacat cgacgctaac atcagtcaac
     1441 agcaagggtt cttcttcagt ccacggataa cgcccttgca aatcagccgt aatatttttc
     1501 gctgtcacca gattaatcac ttcggcaatt cgcaacgtaa cggggccgcg agtccccaga
     1561 tgccaggctc catcggcaaa acggaaaggc aggacaaaat cgacaccgtt aacctgatta
     1621 tctggcatcc aggcactacc gcctttcaac acgccgtgtc ctcccgcgcg gaatccttgt
     1681 tcaggcgcag cagaaaatgc aacctgagca tacagttccc catcgcgtaa gttcatcttc
     1741 cagtcgggtg gcaccagcgg ctggaataca gtcagtgatt gttttggcca ccaggcgtta
     1801 ccgcgcagac gaataccgtc ccagcgacca tttacccgaa ccgggccaat ctcaccagca
     1861 tgtaaatcgc ctttaaagag gaaatacgtc ggatcgcgcc catcgacgct aaattttaag
     1921 gttgatggcg gtaacacact gccgccagtg aacagcgttt gcccggcgtc cagtgacagc
     1981 gcgccgctga aggagggatg ctgtgcgtca cgtacccaga cgacgggctt gtcgagaatt
     2041 aatcgcggct tttctaccgt catcgtaccg tattgtaact ggtcgaaacc ggtggagagg
     2101 tcggtcagcg taatcgtgct gtcatgccat tcaccttttc ctgcgacatc ccaggtggca
     2161 tttatcggtg taaaactgcc ttttccccag tagcgccact gccagcggcc cgcgtcaggg
     2221 agaaaatcat tcgccagccc atccatatgc agcacgaaat cgcccagttc atattcatgc
     2281 gcctgcaaaa tggcctgcaa acgtccgtca acaccacgtt gggtgacttt tacacccgct
     2341 aaaggccagc ggatttcatc gatgtccagc gaatcgatga ctcttccctt cgaacgtaat
     2401 aacgcgcctg gctcaaaggc cagcgttggg tcagtcagac ttccacttag ctgcgcgggt
     2461 aagcgggcat ataaaatgag gtccgcctgt ttcgcttcac cggtcagctg cagaggcagc
     2521 tggctgttat ccatgcttaa ttttcctggg ccaaaattta gcaccgcgtt gcctttaccc
     2581 gcttgcccct gggtcagcac actcagtcgt ccgctgatca gagcgttctc aagccctgcc
     2641 tgccagttgt cgactttgac acccagtcgg ccactcaaag gaaaacctgc atacggccaa
     2701 ctccagcgac catcgcttac ggtcaattgt tgacgagtaa tttgccacgg caaatcgagc
     2761 aacggatcgc cgttatcccg tgccagcaca atcaattgcc cgctattttc ctgccagtcc
     2821 agctctgcat ccaccagtga cggttcctgc ggcaagttta acgtcgcagt agcatgccca
     2881 cttacaggaa gtccatccgg cacgagcggc atagtaaatt cccccaccag tttaaccggc
     2941 ggctgatttt caaacgcgat gacatccagt tcgctgactg taagttgttg ccctttcagc
     3001 tggccttgaa atttaacttt ttcgccctga taacgcagtt gctgaatatc agaggttaat
     3061 gcgagagaga gttttccctg ccattcctgc cagggagaaa aaatcagttt atcgatattg
     3121 atccaggtgt taggcagcat ggactgccac tgcgcgaggg tttttggagc agctggcgat
     3181 tgctccgtct gcggcaattt cgccagacaa gcagaatcaa gttctaccat gccgacgttc
     3241 aataaccagc ggctgggatg tgaaaggctg gcgttggtga tatgcgcaag ctgacaatct
     3301 cccaccagat aacggagatc gggaatgatt aaacctttac gcgtaatgcg tgggctttca
     3361 tctaatgcaa tacgtgtccc gagcggtagc cagatgcccg ccagcgtggg aacccacagc
     3421 ccgagcgtca tcagcagcgt caacggcaca agaataatca gtaataacag cgcgagaacg
     3481 gctttatatt tacccagcat gggtagttaa tatcctgatt tagcgaaaaa ttaagcattc
     3541 aatacgggta ttgtggcatg tttaaccgtt cagttgaagg ttgcgcctac actaagcatt
     3601 gttgttgatg aatttttcaa tatcgccata gctttcaatt atatttgaaa ttttgtaaaa
     3661 tatttttagt agcttaaatg tgattcaaca tcactggaga aagtcttatg aaactcgccg
     3721 tttatagcac aaaacagtac gacaagaagt acctgcaaca ggtgaacgag tcctttggct
     3781 ttgagctgga attttttgac tttctgctga cggaaaaaac cgctaaaact gccaatggct
     3841 gcgaagcggt atgtattttc gtaaacgatg acggcagccg cccggtgctg gaagagctga
     3901 aaaagcacgg cgttaaatat atcgccctgc gctgtgccgg tttcaataac gtcgaccttg
     3961 acgcggcaaa agaactgggg ctgaaagtag tccgtgttcc agcctatgat ccagaggccg
     4021 ttgctgaaca cgccatcggt atgatgatga cgctgaaccg ccgtattcac cgcgcgtatc
     4081 agcgtacccg tgacgctaac ttctctctgg aaggtctgac cggctttact atgtatggca
     4141 aaacggcagg cgttatcggt accggtaaaa tcggtgtggc gatgctgcgc attctgaaag
     4201 gttttggtat gcgtctgctg gcgttcgatc cgtatccaag tgcagcggcg ctggaactcg
     4261 gtgtggagta tgtcgatctg ccaaccctgt tctctgaatc agacgttatc tctctgcact
     4321 gcccgctgac accggaaaac taccatctgt tgaacgaagc cgccttcgat cagatgaaaa
     4381 atggcgtgat gatcgtcaat accagtcgcg gtgcattgat tgattctcag gcagcaattg
     4441 aagcgctgaa aaatcagaaa attgggtcgt tgggtatgga cgtctatgag aacgaacgcg
     4501 atctattctt tgaagataaa tccaacgacg tgatccagga tgacgtattc cgtcgcctgt
     4561 ctgcctgcca caacgtgctg tttaccgggc accaggcatt cctgacagca gaagctctga
     4621 ccagtatttc tcagactacg ctgcaaaact taagcaatct ggaaaaaggc gaaacctgcc
     4681 cgaacgaact ggtttaatct tgccgctccc ctgcattcca ggggagctta ttcagataat
     4741 ccccaaagac ctttcatctt ctattcttaa aatagccctg agtcagaaac tgtaattgag
     4801 aaccacaatg aagaaagtag ccgcgtttgt tgcgctaagc ctgctgatgg cgggatgtgt
     4861 aagtaatgac aaaattgctg ttacgccaga acagctacag catcatcgct ttgtgctgga
     4921 aagcgtaaac ggtaagcccg tgaccagcga taaaaatccg ccagaaatca gctttggtga
     4981 aaaaatgatg atttccggca gcatgtgtaa ccgctttagc ggtgaaggca aactgtctaa
     5041 tggtgaactg acagccaaag ggctggcaat gacccgtatg atgtgcgcta acccgcagct
     5101 taatgaactc gataacacca ttagcgaaat gctgaaagaa ggtgcacaag tggatctgac
     5161 cgcgaaccag ttaacgctgg cgaccgcgaa acagacatta acttataagc tggcggattt
     5221 aatgaattaa tagctgccac agctcccggc ggcaagtgac tgttcgctac agcgtttgcc
     5281 gttgggtaat gcacacatcc caatcgccgt accatccagt tgacgggcaa cagaaagcga
     5341 accgccgatc attgcacaat ttgcttctcc actactggac atcgacgctt ttaaacctgg
     5401 cgctacgtgc gccgcagtcg cctgctgaac aggttcacta ctacacgccg acaacaataa
     5461 agcggcacac cctacccaaa acgctgctcg catctcttct tcctctgatc ttcaagccaa
     5521 acgacaccgc cataaataat aggcagcaca gagggcggcg tcgagagctg tcctgcgcgt
     5581 tgccccgcat ttttactttt ttatggctat ttttttgccc tctgtttgat caaaacattc
     5641 attacgctga tgtgggggac acaaaagcga aaatgcagaa gaaagccatt tgctaaaatt
     5701 gaaggattac tactgggcgc gcagcaattt cgtgcgcccc tcatttgcgc aatgtaaggg
     5761 tgtcatatga ttactattga tggtaatggc gcggttgctt cggtggcgtt tcgcaccagt
     5821 gaagttatcg ccatctaccc tattaccccc agttccacga tggcagaaca ggctgatgcc
     5881 tgggccggaa acggcttgaa gaacgtttgg ggagacacac catgcgtggt tgaaatgcag
     5941 tcggaagcgg gtgctatcgc taccgtgcat ggcgctttgc agacgggtgc cctttcaaca
     6001 tcgtttacgt catcgcaggg tttgctgctg atgatcccga cactgtacaa actggcaggc
     6061 gaactcacac cgtttgtcct gcatgtagcg gcacgtaccg ttgccacaca tgcactctct
     6121 atttttggcg atcattccga cgtcatggcg gtgcgccaga cgggttgcgc gatgttgtgt
     6181 gcggcaaacg tccaggaagc gcaagacttt gctctcattt cgcacatcgc gacgctgaaa
     6241 agccgcgtgc catttattca tttctttgat ggtttccgca cgtcccacga aatcaacaaa
     6301 attgtcccgc tggccgatga cacgattctt gatctcatgc cgcaggccga aattgatgct
     6361 catcgcgccc gggcgctcaa cccggaacat ccggtgatcc gtggtacgtc cgccaatcct
     6421 gacacttatt tccagtctcg cgaagccacc aacccctggt acaacgcagt ctatgaccat
     6481 gttgaacagg cgatgaatga tttctctgcc gcgacaggtc gtcagtatca gccgtttgaa
     6541 tattacgggc atccgcaagc tgaacgggtg attatcctga tgggctcagc cattggcacc
     6601 tgtgaagaag tggttgatga attgctaacc cgtggcgaaa aagtcggcgt gctgaaagtt
     6661 cgcctgtacc gccccttctc agccaaacac ttgctacaaa ctctaccggg atccgtacgc
     6721 agcgtggcgg tactggacag aaccaaagaa cccggtgccc aggcagaacc gctctatctg
     6781 gatgtgatga ccgcactggc agaagccttt aataatggca agcgcgaaac tctgccccgt
     6841 gtcattggtg ggcgctatgg tctttcatcc aaagaatttg gcccggactg tgtactggcg
     6901 gtatttgccg agctcaacgc ggctaaaccg aaagcgcgct ttacggttgg tatttacgat
     6961 gatgtaacca atctgtccct gccgttgccg gaaaacaccc agccaaactc ggcgaaactt
     7021 gaagccttgt tttatggcct tggtagtgat ggcagcgttt ccgcgaccaa aaacaatatc
     7081 aagattatcg gtaattccac gccgtggtac gcacagggct attttgttta cgactccaaa
     7141 aaagcgggcg gcctgacggt ttctcacctt cgcgtgagcg aacagccgat tcgttccgct
     7201 tatctcattt cccaggctga ttttgttggc tgccaccagt tgcagtttat cgataaatat
     7261 cagatggctg agcgtttaaa acctggcggc attttcctgc tcaacacgcc gtacagcgca
     7321 gatgaagtgt ggtcgcgctt gccgcaagaa gttcaggccg tgttaaacca gaaaaaagcg
     7381 cgcttctatg tgattaacgc ggcgaaaatc gcccgcaaat gtggcctggc ggcccgtatt
     7441 aataccgtca tgcagatggc ttttttccat ctgacgcaaa ttctgcctgg cgatagcgcc
     7501 ctcgcagaat tgcagggtgc gattgccaaa agttacagta gcaaaggcca ggatctggtg
     7561 gaacgcaact ggcaggctct ggcgctggcg cgtgaatccg tagaagaagt tccgttgcaa
     7621 ccggtaaatc cgcacagcgc caatcgaccg ccagtggttt ccgatgccgc ccctgatttc
     7681 gtgaaaaccg taaccgcagc gatgctcgca gggcttggtg atgccctccc cgtttcggcg
     7741 ctgccgccag acggcacctg gccgatgggc accactcgct gggaaaaacg caatatcgcc
     7801 gaagagatcc ccatctggaa agaggaactc tgtacccaat gtaaccactg cgtcgcagct
     7861 tgcccacact cagctattcg cgcaaaagtg gtaccacccg aagcgatgga aaacgcccct
     7921 gccagcctgc attcgctgga tgtgaaatcg cgtgatatgc gcgggcagaa atatgtcttg
     7981 caggtggctc cggaagattg caccggttgt aacctgtgcg tcgaagtttg cccggcgaaa
     8041 gaccgtcaga atccagagat taaagccatc aatatgatgt cgcgcctgga acatgtcgaa
     8101 gaagagaaaa tcaattacga tttcttcctc aacctgccag aaatcgaccg tagcaaactg
     8161 gaacgtattg atattcgtac atcgcagctg attacaccgc tgtttgaata ttccggtgct
     8221 tgctccggtt gtggcgagac gccgtatatt aaattgctga ctcagctcta tggcgaccgg
     8281 atgttgatcg ctaacgccac cggctgttct tcaatttatg gcggtaacct gccctctaca
     8341 ccgtatacca ccgatgccaa cggtcgtggg ccggcatggg cgaactctct atttgaagat
     8401 aatgccgaat ttggccttgg tttccgcctg acggtcgatc aacaccgtgt ccgcgtgctg
     8461 cgtctgctgg atcaatttgc cgataaaatc ccggcggaat tactgacggc gttgaaatca
     8521 gacgctacgc cagaggttcg tcgagaacag gtcgcagctt tacgccagca actcaacgat
     8581 gttgccgaag cacatgaact gctacgtgat gcagatgcac tggtggaaaa atcaatctgg
     8641 ctgattggtg gtgatggctg ggcttacgat atcggctttg gcggtctgga tcatgtattg
     8701 agtttgacgg aaaacgtcaa cattctggtg ctggatacgc aatgctattc caacaccggt
     8761 ggtcaggcgt cgaaagcgac accgctgggt gcagtaacta aatttggcga gcacggcaaa
     8821 cgtaaagcgc gtaaagatct tggcgtcagt atgatgatgt acggtcatgt ttatgtggcg
     8881 cagatttctc tcggcgcgca gctgaaccag acggtgaaag cgattcagga agcggaagcg
     8941 tatccgggac catcgctgat cattgcttat agcccgtgtg aagagcatgg ttacgatctg
     9001 gcactcagcc atgaccagat gcgccaactc acagctaccg gcttctggcc gctatatcgc
     9061 ttcgatcctc gtcgtgccga tgaaggcaaa ctgccgctgg cgctggattc acgcccgccg
     9121 tcagaagcac tggaagaaac gttacttcac gagcaacgtt tccgtcgtct gaattcgcag
     9181 cagccagaag tggcagaaca gttatggaaa gatgctgcag ctgatttgca aaaacgctat
     9241 gacttcctgg cacaaatggc cggaaaagcg gaaaaaagca acaccgatta aatgctctgg
     9301 ataaggatta cccaattcta aaaaaaagcc cggacgactg ttcgggcttg tctttttata
     9361 tgttggaaaa tcagtggcaa tgcaatggcc caacagaaaa taaaatctgt gacaataaag
     9421 gcatataacc cgcgcagaat aacgtataga aaattaaaag tataattttt attttgtatg
     9481 tattatcttc gatacattca ccagacttat tatcatttca gaagaattat ttgattaagg
     9541 ttttacttaa ggcgtaacaa atgatattta tcggctaact gaacttctcc tttaggatgt
     9601 tttcactccc cggaatgggg ataaaaggca aataaaataa ctaaaggatt tattcaatga
     9661 aaagcaaagt actggcactt ttaattcctg ccctgctcgc cgcaggtgct gcacatgcag
     9721 ccgaagttta taataaagac ggcaacaaat tagatctgta tggcaaagtt gatggcctgc
     9781 attatttttc tgataattca gcgaaagatg gcgatcagag ctatgcgcgt ctgggtttta
     9841 aaggcgaaac ccaaattaac gatcaactca ctggctacgg tcaatgggaa tacaatattc
     9901 aggcaaacaa cactgaatct tcaaaaaacc agtcatggac ccgtctggca tttgccgggc
     9961 tgaaatttgc agattacggt tctttcgatt acggacgtaa ttatggcgta atgtacgaca
    10021 tcgaaggctg gaccgatatg ctgcctgaat ttggcggtga ctcttatacc aatgcagaca
    10081 actttatgac tggtcgtgcc aacggcgtcg cgacttatcg taatactgat ttcttcggtc
    10141 tggtaaatgg tctgaacttc gcggtgcagt atcaaggtaa caacgaaggt gccagtaatg
    10201 gtcaggaagg caccaacaac ggacgtgatg ttcgccatga aaatggtgac ggctggggtc
    10261 tttccacaac atatgattta ggcatgggct ttagcgctgg tgcggcatac acctcatctg
    10321 accgcaccaa tgaccaggtt aaccatactg cggcgggtgg tgataaagca gacgcgtgga
    10381 ctgctgggct aaaatacgat gctaacaata tttacctggc aaccatgtat tcagaaacgc
    10441 gtaatatgac cccgtttggc gacagcgatt atgctgttgc aaacaaaacc cagaactttg
    10501 aagtcactgc acagtaccag tttgattttg gtctgcgtcc ggcagtctct ttcctgatgt
    10561 ctaaaggccg tgacctgcac gctgccgatg gtgcagacaa cccggcaggt gttgatgata
    10621 aagatcttgt taaatacgcc gatgttggcg cgacttacta tttcaataaa aacatgtcca
    10681 cttatgttga ctataaaatc aacctgttgg atgaagatga cagtttctac gctgccaacg
    10741 gcatctctac cgatgatatt gtcgctttag gtctggttta tcagttctaa atcctcctgc
    10801 ccgctgttat ggcgggcttt ttctgcttat tcttcctcct ttgatctaaa ttaaaaatgt
    10861 gaactccgtc attacacaaa aagtgtcatc tggcgttaca ctttatgcgg atactaaaac
    10921 aggaggtttt atgaacagaa cgattcttgt ccctatcgat atttccgatt cagaattaac
    10981 tcaacgcgtg attagccacg ttgaggcaga ggcaaagatt gacgatgcag aggttcattt
    11041 cctgacggta ataccttcac tgccttacta tgcctctctg ggtttagcgt attccgcaga
    11101 attaccggca atggatgacc tgaaagcgga agccaaatcg caactggaag agatcattaa
    11161 aaaatttaaa ctgccaaccg acagagtgca tgtccatgtt gaggaaggct cgcccaaaga
    11221 ccgcattctg gaattggcga agaagatccc cgctcatatg atcatcattg cttcccatcg
    11281 accggatatc accacttatc tgctcggttc caacgccgca gctgtagtgc gtcacgcaga
    11341 gtgctccgtg ctggttgtgc gctgacacta acgcccgcac attgctgcgg gctttttgat
    11401 tcatttcgca aatgtgctga cattttcccc tctaatccgt accatacacg ccacagtttt
    11461 atatcagatt tcttatgctg ggcgttccgg catgacgcat actcttctga tgccatataa
    11521 cgaattgagt cgcttttaaa tgtcgcaaaa tcaagaaatt agtaagaaag aacaatacaa
    11581 cctgaacaag taagggcaaa aatcacaact atctgaccca taagtatttt atttattttt
    11641 caatatgtta aacattatca gcgacaacaa taagctacga taacccactc tttttttgcc
    11701 ccttttttgc cccttctaca gcgttttgcc ccatttttgc catcaacaat tttcctcata
    11761 aagtcatttc aaagcctaat agctgctcta cttttttcct caatatcatg tcgccagcag
    11821 gaaacaacct aatccctgtt ttaggcctca aagctgttaa tgataatcat tttcattttg
    11881 tatgggttat gctataaaac atagccgttg ctatgtttgt tctaattttg ctcactatag
    11941 gtactaaatt atgcactcaa taaaaaaagt aaccatgctc ttggggtgcc tcgccctcac
    12001 ctgctcgatc gcatttcagg caagtgcaac tgaaaaattc aaggtcatta caacatttac
    12061 catcatcgcg gatatggcca aaaacgtggc tggagatgct gcagaagtct catccataac
    12121 caagcctggt gcagaaattc atgagtatca gcctacccct ggcgatatta aacgtgcgca
    12181 gggggcacaa ctgattctcg ccaatggtat gaatctggaa ttgtggttcc aacgctttta
    12241 ccagcatctc aatggggttc cagaagtaat tgtctcttcg ggtgtgacgc cagtagggat
    12301 caccgaagga ccctatgagg gcaaacctaa tccccatgca tggatgtcgc cagataatgc
    12361 tctgatttac gtcgataata ttcgtgatgc gttgataaaa tacgatccgg caaatgcaca
    12421 aacctaccaa cgtaatgccg atacttataa agccaagatt acccaaaccc ttgcccccct
    12481 gcgtaagcag attacggaac tccctgagaa tcagcgatgg atggtcacca gtgaaggggc
    12541 tttttcttat ctcgcacgcg atttggggct aaaagagctt tatctgtggc cgattaatgc
    12601 cgatcaacaa ggaacaccgc agcaggtacg taaggttgtt gatatagtta agaaaaatca
    12661 tatcccggca gtctttagcg agagtacgat ttccgataaa ccagcgcgtc aggttgcgcg
    12721 tgaaaccggc gcacactacg gcggtgtgct ctatgtcgat tccctgagca cagaaaacgg
    12781 cccggtacct acgtatatcg accttcttaa ggttactacc agtacgctgg tacagggaat
    12841 taaagccggg aaaagggaga aataatgatg caatctgcag gcattgtcgt taatgatgtt
    12901 actgtcacct ggcgtaacgg gcacacagca ctgcgcgatg catccttcac agtaccaagt
    12961 ggatctattg ccgctctggt tggggtaaac ggttccggaa aatcgacgtt gtttaaagcg
    13021 attatgggat ttgtgcgtct gaccagcgga aagatatctg ttctgggtat tcccacgcga
    13081 caggcgctac agaaaaacct ggttgcctac gtccctcagt cagaagaggt tgactggtca
    13141 tttcctgtac tggtggaaga tgtggtgatg atgggccgct atgggcatat gggtatgttg
    13201 cgtatagcca aaaagaggga tcgtcagatt gtcaccgatg cgctggaacg tgtcgatatg
    13261 gtggattttc gccatcgtca aatcggcgag ctttccggtg gacaaaagaa acgcgtcttt
    13321 ctggcgagag cgattgcaca gcagggtgat gtgatcctgc tggatgaacc ttttaccggc
    13381 gttgatgtga aaaccgaagc gaaaatcatc agcctgttga gggagttacg cgcggaaggc
    13441 aaaacgatgc ttgtctcaac gcacaatctc ggctcagtca cgacattttg cgattatacg
    13501 gtcatggtca aaggcaccgt cctggcaagc gggccgacag acaccacttt tacagccgag
    13561 aacctggagc tggcttttag cggcgtactg cgccatgtca cacttaacga ctcggaagaa
    13621 agcattatta ctgacgatga acgccctttt gtggcacatc gaccgtcagc ggtgcagagg
    13681 gaagcaagat gaacgtgcta ctggaaccct tcagctatga atatatgctc aatgcgatgt
    13741 gggtctcggc aatggtcggc ggtctctgcg ctttcctgtc atgctatctg atgctaaaag
    13801 gctggtcgct gattggcgat gcgctgtcgc actctattgt cccgggtgtt gcgggggcat
    13861 atatgctggg gcttccgttt tctctgggag ccttcttttc cggcggactg gcagcgggca
    13921 gcatgctgtt tcttaaccaa cgcacccgct taaaagaaga tgccatcatc ggcctgatct
    13981 tctcgtcctt ttttggcctg gggctgttta tggtgtcatt gaacccgaca tccgtgaaca
    14041 ttcagaccat cgtcctgggc aatattctgg caatcgatcc ggcggatatc ctgcaactca
    14101 ctatcattgg cattctctcg atcatcgtgc tttttttcaa atggaaagat cttatggtga
    14161 cctttttcga cgagaatcac gcccgtgcta ttggattaca tccgggcaga ttaaaaatcc
    14221 tcttcttcac cctgctgtcg gtttcgaccg ttgcggcgct gcaaacggtt ggtgcctttc
    14281 tggtgatctg tctggtggtg actcccggtg caacagcatg gttgttaacc gatcgttttc
    14341 cccgcctgct cataattgct gtaactattg gcagtgtaac cagtttcctc ggcgcgtggg
    14401 tcagttactt tctggacggc gcgacaggtg gaatcatcgt ggtggcgcag acactgctgt
    14461 ttcttttagc atttgtattt gcgcccaccc atggactttt ggcaaatcgt cggcgtgcgc
    14521 ataaagcgct ggaggatcgc tcatgatggc attattactg gagccactgc agttcacctt
    14581 tatgagtcac gcactgctga tttcactggt agtttccata ccctgtgcgc tgctgtcggt
    14641 ctttctggtc ctgaaaggtt gggcactgat gggtgacgcg atgagtcatg cggtttttcc
    14701 tggcatcgtg cttacctgga ttctggggtt acctctggct acaggggcat tcgtcgccgg
    14761 agtattctgc gcggtggcga cgggatacct gaaagacaac agccgaatca agcaagatac
    14821 agttatgggg attgtttttt ctggcatgtt tgccgcaggt ctgatcttgt atattgcaat
    14881 caaaccagac gtacatcttg accatattct tttcggcgac atgctcggga taaccatcgg
    14941 cgatataatc cagacggtga ttattgccgg gctggttaca cttgttatta gcgcaaaatg
    15001 gcgggatttt ttgctgttca gctttgatta tcaacaggcg caggtaagcg gtttgcatac
    15061 gagatggctg cattatgggc tgctgtgcat ggtttccctg accattgtgg cgacgctgaa
    15121 agcagtaggt atcattctct ctatttctct gctcattgcg cctggcgcca ttgcagtact
    15181 cctgacacaa cgctttcata ttgcgctcct actggcgact ggcatatcgg taatagtgtc
    15241 aatgactggt gtctggctgt ccttttttat cgacagcgca ccagctccga cgattgtcgt
    15301 cttattcgcg gtcgtgttca tcatgacgtt taccgttacc agcatcaacg cacgcacaaa
    15361 gggaaacgcc catacacagg atctgttatc acccaattag cacaacatcc gaggacagca
    15421 tcgtccgcca gcctacagaa gtccggaaag ccatggaagg tcgttgggag gaggtacgaa
    15481 ttctatcatg caaaaaatac gtaaaatcga taacgcctag aaatcattca atactcgcac
    15541 tatcgaaagt ttaccagcca accgcggcac gttcctgcat acgacgtgcc gcagcttctg
    15601 ttattccacg ataaaattcc taaccgaatt caaatccttc agactattca gctcttcctt
    15661 cagctcccgc tgacggcgat aaatctcgtc attgcgctca acctgtgcct gagccattgc
    15721 tgccgccagt tcttccagtt ccggcatcga cagtttcacc tgctgattat cggcatcacc
    15781 ccacgtcatc acgtcccgta caatgtcgga tttcgcagcc attaccaccg gataaaggcg
    15841 gcccagtgag ttggggccag cattccagat acgaccgttc cattcaaacg tgaacggctt
    15901 cgcctcctgt tctgtgcgcc atgcttcaat tccctgacgt ctggcctctc tggccgcttc
    15961 cagcatttct ggtgtcacag tgaatggggc tatctcaccc catttgccac tttgcagttc
    16021 ctgccagatt cgctgacccg tcggtgcgac atcatcagcg gtggctgtgt aggggactgc
    16081 ctggtccctg tcgtcaaaaa aaacgtcaca gtctactgcg ccactttcgg tataacgggg
    16141 attaatgatt tttttaattt ccacggtgca ttcctcacga tgtgcgaata aaaagcccgg
    16201 gcattgcgcc agagac
//
LOCUS       DAESHI010000093        16070 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.094, whole genome shotgun sequence.
ACCESSION   DAESHI010000093 DAESHI010000000
VERSION     DAESHI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16070
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.094"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(1..563)
                     /locus_tag="KW011_003093"
                     /pseudo
     CDS             complement(1..563)
                     /locus_tag="KW011_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(797..2217)
                     /gene="espX1"
                     /locus_tag="KW011_003094"
                     /pseudo
     CDS             complement(797..2217)
                     /gene="espX1"
                     /locus_tag="KW011_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
     gene            complement(2702..2965)
                     /gene="rpsT"
                     /locus_tag="KW011_003095"
     CDS             complement(2702..2965)
                     /gene="rpsT"
                     /locus_tag="KW011_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBH7661371.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            3068..3286
                     /locus_tag="KW011_003096"
     CDS             3068..3286
                     /locus_tag="KW011_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HBH7661372.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            3294..4235
                     /gene="ribF"
                     /locus_tag="KW011_003097"
     CDS             3294..4235
                     /gene="ribF"
                     /locus_tag="KW011_003097"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBH7661373.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            4278..7094
                     /gene="ileS"
                     /locus_tag="KW011_003098"
     CDS             4278..7094
                     /gene="ileS"
                     /locus_tag="KW011_003098"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBH7661374.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            7094..7588
                     /gene="lspA"
                     /locus_tag="KW011_003099"
     CDS             7094..7588
                     /gene="lspA"
                     /locus_tag="KW011_003099"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBH7661375.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            7713..8162
                     /gene="fkpB"
                     /locus_tag="KW011_003100"
     CDS             7713..8162
                     /gene="fkpB"
                     /locus_tag="KW011_003100"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBH7661376.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            8164..9114
                     /gene="ispH"
                     /locus_tag="KW011_003101"
                     /gene_synonym="lytB"
     CDS             8164..9114
                     /gene="ispH"
                     /locus_tag="KW011_003101"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBH7661377.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            9180..10094
                     /gene="rihC"
                     /locus_tag="KW011_003102"
     CDS             9180..10094
                     /gene="rihC"
                     /locus_tag="KW011_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HBH7661378.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            10261..11082
                     /gene="dapB"
                     /locus_tag="KW011_003103"
     CDS             10261..11082
                     /gene="dapB"
                     /locus_tag="KW011_003103"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBH7661379.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNNL"
     gene            11538..12686
                     /gene="carA"
                     /locus_tag="KW011_003104"
     CDS             11538..12686
                     /gene="carA"
                     /locus_tag="KW011_003104"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBH7661380.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            12704..15925
                     /gene="carB"
                     /locus_tag="KW011_003105"
     CDS             12704..15925
                     /gene="carB"
                     /locus_tag="KW011_003105"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBH7661381.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
BASE COUNT         3918 a         3783 c         4258 g         4111 t
ORIGIN      
        1 tccccccagt agaaaggaat ggagataaac agattaaaac gctcctcttc tttattattt
       61 tcatcataag attggctggc agagaaagta taactaatgt gttgccagtt attggagtaa
      121 ctgaattggt aatctttagc atttccactt cgcccccagt aattccgcca aagcgcactt
      181 aatgatacat ttcccagatt attggataaa ggttgcataa tattggcaga aaggctattt
      241 tttctgccaa tatcataaaa atcatcataa tcactctgat gatagtgttt atcattttcg
      301 taaagatggt cgctgaatgt cctgtaatcc tgcgaagcat aacgccaggc ggccacgcta
      361 aatcgagtgg ctgtttgtac tagatattta ttataggcga cctgatagct cgtaccttcg
      421 tgtctggtat cattatttaa cttactactc gatcgggcag cgtaaaaaga gatggcaccc
      481 agcggtgtat tccaaccatt tcccagagtt atagcattat agttatcgga taaaatcgta
      541 cccgattatg tgatcaggtt gttattattt agattaatgt ttaaattttt taatttcttg
      601 atgtgaagtt gatgaaagtt tgaaaattta atattcttaa ttgttccctt ctttataaga
      661 agagcaaggt gccatcatta gagtgacaga gtaggatgtt tcagagcatg cttcagattg
      721 ttttttattt ctctgcagtg tattaataat catcacagat aatttcattc taaaattacc
      781 tctttctttg agtgttttaa tcctgatgaa ctatattttt tataaggagc atgcactcgt
      841 tgatgctctg aaggtatttt ctcatttttg atgatgtttt gtcgtaatcg tgcataaact
      901 tattaagttc tttatacaca tcattattta gctttacatt atcgcgaact aaagtttgta
      961 ccaatggtat caaaatttcg aaacgggacg gtttgtcatc aatacttgcc agggttttga
     1021 atactcctgt gtatctgtaa cctaagtgct tattgacaag ttgagtggtc catcggccag
     1081 gcataatatc ttcacagcgc aaatttttta tcatggtatt agtgaacgat gttccaaata
     1141 tgttgctttt tttaatgaag aggttgttta tttctccatt tgaaaagatg gtgtttgata
     1201 agtcgctttc aaaacatgat atattggtta atacacaaca aaaataatac ccatatgaaa
     1261 ggttgctctt ttctattgtt atatttgata gcaacatttt atgtatatta atattaaggg
     1321 cagtcaaata ctggatcttt tgatcgttaa gttcatacat ctccagtgat ttattatagg
     1381 gtaataaaag tataaattcc tttatattat ctccgcagta agaaaagacg acattaaatt
     1441 tattagtggc catgttttca gttgagcagt aagctgattt ttctattaac cgactgggca
     1501 cgcttttaaa taaagataat tttccacata gttttgaata tgccgaattg ctagttttaa
     1561 agttctgcca cattattccc tggttatcat ctatcgatga ggaggggatt ttattgcatt
     1621 gctcattgta tagctcaggg aaaatatttt ttttaataag ctttttgatt tcctggttaa
     1681 gatgactttt tttcttgtct attgcactgt attttgcgtt ttgtgacgtt tcctgatgat
     1741 cataaaatga ctggagattt ctgcaggcct cataaaatat ttcaggagta tatagctgtg
     1801 ggtatatttc ttggttgaag tttgccttta aaaaattaac gaatttttca tggcattctt
     1861 ggtatatctt aatctcttca gaaagacatt cgcttgttct gtcatcaaaa acatccaatc
     1921 taagtaatgt gatacctttg tgtttaaaaa ataaaattga actgtatgtt ctatgagttt
     1981 acatctataa aaatatgcat ctctcagatg agtatattga aaaaaatgat gaaggactgg
     2041 agatggttta ggcaagttat ggcttctaaa aaagtcatca aacaaaatta agaactctgt
     2101 tttatactcg caaaatactt ttactaactc attttgtctt gttttattta ttattacatc
     2161 atctactata tttgatttat aaagacaatg tggcgaagtt gagataccat ttgtcattat
     2221 cagtccttga agttgatgaa tttttattaa tttaatttat gagtgaatgt gactactatc
     2281 aaaaatagct cattaatttt atgaagtaga atataaatag gtatttaacc tgtgttgatt
     2341 gttgttaatt atttacaaga tctaataagg agatatttat gaatggttaa agtgtatagg
     2401 aatttaattc aaaaatagag tggtcttaag tttaatacct caatgtgtat cacaatatcc
     2461 acattctttg taggggagtc tggagattga gtagatattc ttgttcagaa tgtatcagcc
     2521 gatggttcta cgattcttaa gccacgaaga gttcagaaag tgtaacggca tgtctgcaaa
     2581 agccagcatc ttttgactat ctggcaaccg gtagtgtgtt ctctcacgca tcataaaagc
     2641 agcaggcata aaaaaaaccc gcttgcgcgg gctttttcac aaagcttcag caaattggcg
     2701 attaagccag tttgttgatc tgtgcagtca ggttagcctt atgacgtgca gctttgtttt
     2761 tgtggatcag acctttagca gcctgacggt ccacgatcgg ttgcatttcg ttaaatgctt
     2821 tctgtgcagc agctttgtcg ccagcttcga tagctgcgta tactttcttg atgaaagtac
     2881 gcatcataga gcgacggctt gcgttgtgct tacgagcctt ttcagactga atggcgcgct
     2941 tcttagctga tttgatatta gccaaggtcc aactcccaaa tgtgttctat atggacaatt
     3001 caaaggccga ggaatatgcc cttttagcct tcttttgtca atggatttgt gcaaataagc
     3061 gccgttaatg tgccggcact cgttacgtag tgatggcgca ggattctacc agcttgcggg
     3121 gtgtgaatac agcttttccg cgataaaaat tgcagcaggc ggtcactttc ttcccgtgat
     3181 ttgcgccatg gcaatgaaaa gccacttctt tctgatttcg gtactcaatc gccggttaac
     3241 cttgaccgct gtacaaggta tactcggacg attttcactg ttttgagcca gacatgaagc
     3301 tgatacgcgg catacataat ctcagccagg ccccgcaaga agggtgtgtg ctgactattg
     3361 gtaatttcga cggcgtgcat cgcggtcatc gcgcgctgtt acagggcttg caggaagaag
     3421 ggcgcaagcg caacttaccg gtgatggtga tgctttttga acctcaacca ctggaactgt
     3481 ttgctaccga taaagccccg gcaagactga cccggctgcg ggaaaaactg cgttaccttg
     3541 cagagtgtgg cgttgattac gtgctgtgcg tgcgtttcga caggcgtttc gcggcgttaa
     3601 ccgcgcaaaa tttcatcagc gatcttctgg tgaagcattt gcgcgtaaaa tttcttgccg
     3661 taggggatga tttccgcttt ggcgctggtc gtgaaggcga tttcttgtta ttacagaaag
     3721 ctggcatgga atacggcttc gatatcacta gtacgcaaac tttttgcgaa ggtggcgtgc
     3781 gtattagcag caccgctgtg cgtcaggcgc tggcggatga caatctggct ctggcagaaa
     3841 gtttactggg gcacccgttt gccatctccg ggcgtgtagt ccacggtgat gaattagggc
     3901 gcactatagg tttcccgacg gcgaatgtac cgctacgccg tcaggtttcc ccggtgaaag
     3961 gggtttatgc ggtagaagtg ctgggcctcg gtgaaaagcc gttacccggc gtggcaaaca
     4021 tcggaacacg cccaacggtt gccggtattc gccagcagct ggaagtgcat ttgttagatg
     4081 ttgcaatgga cctttacggt cgccatatac aagtagtgct gcgtaaaaaa atacgcaatg
     4141 agcagcgatt tgcgtcgctg gacgaactga aagcgcagat tgcgcgtgat gaattaaccg
     4201 cccgcgaatt ttttgggcta acaaaaccgg cttaagcctg ttatgtaatc aaaccgaaac
     4261 acggaaccga gaatctgatg agtgactata aatcaaccct gaatttgccg gaaacagggt
     4321 tcccgatgcg tggcgatctc gccaagcgcg aaccgggaat gctggcgcgt tggactgatg
     4381 atgatctgta cggcatcatc cgtgcggcta aaaaaggcaa aaaaaccttc attctgcatg
     4441 atggccctcc ttatgcgaat ggcagcattc atattggtca ctcggttaac aagattctga
     4501 aagacattat cgtgaagtcc aaagggcttt ccggttatga ctcgccgtat gtgcctggct
     4561 gggactgcca cggtctgccg atcgagctga aagtagagca agaatacggt aagccgggtg
     4621 agaaattcac cgccgccgag ttccgcgcca agtgccgcga atacgcggcg acccaggttg
     4681 acggtcaacg caaagacttt atccgtctgg gcgtgctggg cgactggtcg cacccgtacc
     4741 tgaccatgga cttcaaaact gaagccaaca tcatccgcgc gctgggcaaa atcatcggca
     4801 acggtcacct gcacaaaggc gcgaagccag ttcactggtg cgttgactgc cgttctgcgc
     4861 tggcggaagc ggaagttgag tattacgaca aaacttctcc gtccatcgac gttgcttttc
     4921 aggcggtcga tcaggatgca ctgaaagcaa aatttgccgt aagcaacgtt aacggcccaa
     4981 tctcgctggt gatctggacc actacgccgt ggactctgcc tgcgaaccgc gcaatctcta
     5041 ttgcacctga tttcgactat gcgctggtgc agatcgacgg tcaggccgtg attctggcga
     5101 aagatctggt tgaaagcgta atgcagcgta tcggcgtgac cgattacacc attctcggca
     5161 cggtaaaagg tgcggagctt gagttgctgc gctttaccca tccgtttatg ggcttcgacg
     5221 ttccggcaat cctcggcgat cacgttaccc tagatgccgg taccggtgcc gttcacaccg
     5281 cgcctggcca cggcccggac gactatgtga tcggtcagaa atacggcctg gaaaccgcta
     5341 acccggttgg cccggacggc acttatctgc cgggcactta tccgacgctg gatggcgtga
     5401 acgtcttcaa agcgaacgac atcgtcgttg cgctgctgca ggaaaaaggc gcgctgctgc
     5461 acgttgagaa aatgcagcac agctatccgt gctgctggcg tcacaaaacg ccgatcatct
     5521 tccgcgcgac gccgcagtgg ttcgtcagca tggatcagaa aggtctgcgt gcgcagtcac
     5581 tgaaagagat caaaggcgtg cagtggatcc cggactgggg ccaggcgcgt atcgagtcga
     5641 tggttgctaa ccgtcctgac tggtgtatct cccgtcagcg cacctggggc gtaccgatgt
     5701 cactgttcgt gcacaaagac acggaagagc tgcatccgcg tactctcgaa ctaatggaag
     5761 aagtggcaaa acgcgttgaa gttgacggca tccaggcgtg gtgggatctt gatgcgaaag
     5821 agatcctcgg cgatgaagct gatcagtacg tgaaagtgcc ggacacattg gatgtatggt
     5881 ttgactccgg atctacccac tcttctgttg ttgacgtgcg tccggaattt gccggtcacg
     5941 cagcggacat gtatctggaa ggttctgacc agcaccgtgg ttggttcatg tcttccctaa
     6001 tgatctccac cgcgatgaaa ggcaaagcgc cgtatcgtca ggtactgacc cacggcttta
     6061 ccgtggatgg tcagggtcgc aagatgtcta aatccatcgg caataccgtt tcgccgcagg
     6121 atgtgatgaa caaactgggc gcggatattc tgcgtctgtg ggtggcatca accgactaca
     6181 ccggtgaaat ggccgtttct gacgagatcc tgaaacgtgc tgccgatagc tatcgtcgta
     6241 tccgtaacac cgcgcgcttc ctgctggcaa acctgaacgg ttttgatcca gcaaaagata
     6301 tggtgaaacc ggaagagatg gtggtactgg atcgctgggc cgtaggttgt gcgaaagcgg
     6361 cacaggaaga catcctcaag gcgtacgaag catacgattt ccacgaagtg gtacagcgtc
     6421 tgatgcgctt ctgctccgtt gagatgggtt ccttctacct cgacatcatc aaagaccgtc
     6481 agtacaccgc caaagcggac agtgtggcgc gtcgtagctg ccagactgcg ctgtatcaca
     6541 tcgcagaagc gctggtgcgc tggatggcac caatcctctc cttcaccgct gatgaagtgt
     6601 ggggctacct gccgggcgaa cgtgaaaaat acgtcttcac cggtgagtgg tacgaaggcc
     6661 tgtttggcct ggcagacagt gaagcgatga acgatgcgtt ctgggacgag ctgttgaaag
     6721 tgcgtggcga agtgaacaaa gtcattgagc aagcgcgtgc cgacaagaaa gtgggtggct
     6781 cgctggaagc ggcagtaacc ttgtatgcag aaccggaact gtcggcgaaa ctgaccgcgc
     6841 tgggcgatga attacgattt gtcctgttga cctccggcgc taccgttgca gactataacg
     6901 acgcacctgc tgatgctcag cagagcgaag tactcaaagg gctgaaagtc gcgttgagta
     6961 aagccgaagg tgagaagtgc ccacgctgct ggcactacac ccaggatgtc ggcaaggtgg
     7021 cggaacacgc agaaatctgc ggccgctgtg tcagcaacgt cgccggtgac ggtgaaaaac
     7081 gtaagtttgc ctgatgagtc aatcgatctg ttcaacaggg ctacgctggc tgtggctggt
     7141 ggtagtcgtg ctgattatcg atctgggcag caaatacctg atcctccaga actttgctct
     7201 gggggatacg gtcccgctgt tcccgtcgct taatctgcat tatgcgcgta actatggcgc
     7261 ggcgtttagt ttccttgccg atagcggcgg ctggcagcgt tggttctttg ccggtattgc
     7321 gattggtatt agcgtgatcc tggcagtgat gatgtatcgc tcgaaggcca cgcagaagct
     7381 aaacaatatc gcttacgcgc tgattattgg cggcgcgctg ggcaacctgt tcgaccgcct
     7441 gtggcacggc ttcgttgtcg atatgatcga cttctacgtc ggcgactggc acttcgccac
     7501 cttcaacctt gccgatactg ccatctgtgt cggtgcggca ctgattgtgc tggaaggttt
     7561 tttgccttct agagcgaaaa aacaataata aaccctgccg gatgcgatgc tgacgcatct
     7621 tatccggcct acagattgct gcgaaatcgt aggccggata aggcgtttac gccgcatccg
     7681 gcaaaaatcc tcaaatataa gagcaaacct gcatgtctga atctgtacag agcaatagcg
     7741 ccgtcctggt gcacttcacg ctaaaactcg acgatggcac caccgccgag tctacccgca
     7801 acaacggtaa accggcgctg ttccgcctgg gtgatgcttc tctttctgaa gggctggagc
     7861 aacacctgtt ggggctgaaa gtgggcgata aaaccacctt ctcgttggag ccagatgcgg
     7921 cgtttggcgt gccgtcaccg gacctgattc agtacttctc ccgccgtgaa tttatggatg
     7981 caggcgagcc agaaattggc gcaatcatgc tttttaccgc aatggatggc agtgagatgc
     8041 ctggcgtgat ccgcgaaatt aacggcgact ccattaccgt tgatttcaac catccgctgg
     8101 ccgggcagac cgttcatttt gatattgaag tgctggaaat cgatccggca ttggaggcgt
     8161 aacatgcaga tcctgttggc caacccgcgt ggtttttgtg ccggggtaga ccgcgctatc
     8221 agcattgttg aaaacgcgct ggccatttac ggcgcaccga tatatgtccg tcacgaagtg
     8281 gtacataacc gctatgtggt cgatagcttg cgtgagcgtg gggctatctt tattgagcag
     8341 attagcgaag taccggacgg cgcgatcctg attttctccg cacacggtgt ttctcaggcg
     8401 gtacgtaacg aagcaaaaag ccgcgatttg acggtgtttg atgccacctg tccgctggtg
     8461 accaaagtgc atatggaagt cgcccgcgcc agccgtcgtg gcgaagagtc gattctcatc
     8521 ggccacgccg ggcatccgga agtggaaggt acaatgggcc agtacagtaa cccggaaggg
     8581 ggaatgtatc tggtcgaatc gccggacgat gtgtggaaac tgacggtcaa aaacgaagag
     8641 aagctctcct ttatgaccca gaccacgctg tcggtggatg acacgtctga tgtgatcgac
     8701 gcgctgcgta aacgcttccc gaaaattgtc ggtccgcgca aagatgacat ctgctacgcc
     8761 acgactaacc gtcaggaagc ggtacgcgcc ctggcagaac aggcggaagt tgtgttggtg
     8821 gtcggttcga aaaactcctc caactccaac cgtctggcgg agctggccca gcgtatgggc
     8881 aaacgcgcgt ttttgattga cgatgcgaaa gatatccagg aagagtgggt gaaagaggtt
     8941 aaatgcgtcg gcgtgactgc gggcgcatcg gctccggata ttctggtgca aaatgtggtg
     9001 gcacgtttgc agcagctggg cggtggtgaa gccattccgc tggaaggccg tgaagaaaac
     9061 attgttttcg aagtgccgaa agagctgcgt gtcgatattc gtgaagtcga ttaagtcatt
     9121 agcagcctaa gttatgcgaa aatgccggtc ttgttaccgg cattttttat ggagaaaaca
     9181 tgcgtttacc tatcttcctc gatactgacc ccggcattga cgatgccgtc gccattgccg
     9241 ccgcgatttt tgcccccgaa ctcgacctgc aactgatgac caccgtcgcg ggtaatgtct
     9301 cggttgagaa aaccacccgc aacgccctgc aactgctgca tttctggaat gcggagattc
     9361 cgctcgccca gggagccgct gtgccgctgg tacgcgcacc gcgtgatgca gcatctgtgc
     9421 acggcgaatc gggaatggct ggctacgact ttgttgagca caaccgaaag ccaatcggga
     9481 taccagcgtt tctggcgatt cgtgatgccc tgatgcgtgc accagagcct gttaccctgg
     9541 tggccattgg cccgttaacc aatattgcgc tgttactttc acaatgcccg gaatgtaaac
     9601 cgtatattcg tcgtctggtg atcatgggcg gttcttctgg tcgcggcaac tgtacaccaa
     9661 atgccgagtt taatatcgct gccgatccag aagcggctgc ctgtgtcttc cgtagcggaa
     9721 tcgaaatcgt catgtgcggt ctggatgtca ccaatcaggc aatattaacc cctgactatc
     9781 tcgctacttt gccggagttg aatcgcaccg gaaaaatgct tcatgcactg tttagccact
     9841 accgtagcgg cagtatgcaa agcggtctgc gaatgcacga tctctgcgcc atcgcctggc
     9901 tggtgcgccc ggacttattc accctcaagc cgtgttttgt cgcggtagaa actcaaggcg
     9961 aatttacctc aggcacgacg gtggttgata tcgacggttg cctgggcaaa ccagccaatg
    10021 taaaggtggc attggatctg gatgtgaaag gcttccagca gtgggtggct gaggtgctgg
    10081 ctctggcgtc gtaacctgtc acatgttatc ggcatgcagt cattcatcga ctcatgcctt
    10141 tcactgatat ccctccctgt ttatcattaa tttctaatta tcagcgtttt tggctggcgg
    10201 cgtagcgatg cgctggttac tctgaaaacg gtctatgcaa attaacaaaa gagaatagct
    10261 atgcatgatg caaacatccg cgttgccatc gcgggagccg gggggcgtat gggccgccag
    10321 ttgattcagg cggcgctggc attagaaggc gtgcagctgg gcgcggcgct ggagcgcgaa
    10381 ggatcttctt tactgggcag cgacgccggt gagctggctg gtgcagggaa aacaggcgtt
    10441 accgtgcaaa gcagcctcga tgcggtaaaa gatgattttg atgtgtttat cgattttacc
    10501 cgtccggaag gtacgctgaa ccatctcgct ttttgtcgcc agcatggcaa agggatggtg
    10561 atcggcacta cggggtttga cgaagccggt aaacaggcga ttcgtgacgc cgccgccgat
    10621 attgcgattg tctttgccgc caattttagc gttggcgtta acgtcatgct taagctgctg
    10681 gagaaagcag ccaaagtgat gggtgactac accgatatcg aaattattga agcacatcat
    10741 agacataaag ttgatgcgcc gtcaggcacc gcactggcaa tgggagaggc gatcgcccac
    10801 gcccttgata aagatctgaa agattgcgcg gtctacagtc gtgaaggcca caccggtgaa
    10861 cgtgtgcctg gcaccattgg ttttgccacc gtgcgtgcag gtgacatcgt tggtgaacat
    10921 accgcgatgt ttgccgatat tggcgagcgt ctggagatca cccataaggc gtccagccgt
    10981 atgacatttg ctaacggcgc ggtaagatcg gctttgtggt tgagtggtaa ggaaaatggt
    11041 ctttttgata tgcgagatgt acttaatctc aataatttgt aaccacaaaa tatttgttat
    11101 ggtgcaaaaa taacacattt aatttattga ttataaaggg ctttaatttt tggccctttt
    11161 atttttagtg ttatgttttt aaatattcta taagtgccaa aaattacatg ttttgtcttc
    11221 tgtttttgtt gttttaatgt aaattttgac catttggtcc actttttcct gctcgttttt
    11281 atttcatgca atcttcttgc tgcgcaagcg ttttccagaa caggttatat gatctttttg
    11341 tcgcttaatg cctgtaaaac atgcatgagc cacaaaataa tataaaaaat accgccatta
    11401 agttgacttt tagcgcccat atctccagaa tgccgccgtt tgccagaaat tcgtcggtaa
    11461 gcagatttgc attgatttac gtcatcattg tgaattaata tgcaaataaa gtgagtgaat
    11521 attctctgga gggtgttttg attaagtcag cgctattggt tctggaagac ggaacccagt
    11581 ttcacggtcg ggccataggg gcaacaggtt cggcggttgg ggaagtcgtt ttcaatactt
    11641 caatgaccgg ttatcaagaa atcctcactg atccttccta ttctcgtcaa atcgttactc
    11701 ttacttatcc ccatattggc aatgtcggca ctaatgacgc cgatgaagaa tcttctcagg
    11761 tacatgcaca aggtctggtg attcgcgatc tgccgctgat tgccagcaac ttccgtaata
    11821 ccgaagacct ctcttcttac ctgaagcgcc ataacatcgt ggcgattgcc gatatcgata
    11881 cccgtaagct gacgcgttta ctgcgcgaga aaggcgcaca gaatggctgc attatcgcag
    11941 gcgataaccc ggatgcggcg ttggcgttag aaaaagcccg cgcgttccca ggtctgaatg
    12001 gcatggatct ggcaaaagaa gtgaccaccg cagaagccta tagctggaca caagggagct
    12061 ggacgttgac cggcggcctg ccagaagcga aaaaagaaga cgagctgccg ttccacgtcg
    12121 tggcttatga ttttggtgcc aagcgcaaca tcctgcggat gctggtggat agaggctgtc
    12181 gcctgaccat cgttccggcg caaacttctg cggaagatgt gctgaaaatg aatccagacg
    12241 gcatcttcct ctccaacggt cctggcgacc cggccccgtg cgattacgcc attaccgcca
    12301 tccagaaatt cctcgaaacc gatattccgg tattcggcat ctgcctcggt catcagctgc
    12361 tggcgctggc gagcggtgcg aagactgtca aaatgaaatt tggtcaccac ggcggcaacc
    12421 atccggttaa agatgtggag aaaaacgtgg tgatgatcac cgcccagaac cacggttttg
    12481 cggtggatga agcaacatta cctgcaaacc tgcgtgtcac gcataaatcc ctgttcgacg
    12541 gtacgttaca gggcattcat cgcactgata aaccggcgtt cagcttccag ggtcacccgg
    12601 aagccagccc tgggccacac gacgcagcgc cgttgttcga ccactttatc gagttaattg
    12661 agcagtaccg taaaaccgct aagtaatcag gagtaaaaga gccatgccaa aacgtacaga
    12721 tataaaaagt atcctgattc tgggtgcggg cccgattgtt atcggtcagg cgtgtgagtt
    12781 tgactactct ggcgcgcaag catgtaaagc cctgcgcgaa gagggttacc gcgtcattct
    12841 ggtgaactcc aacccggcga ccatcatgac cgacccggaa atggccgatg cgacctacat
    12901 cgagccgatt cactgggaag tagtacgcaa gattattgaa aaagagcgcc cggacgcggt
    12961 gctgccaacg atgggcggtc agacggcgct gaactgcgcg ctggagctgg agcgtcaggg
    13021 cgtgttggaa gagttcggcg tgactatgat tggtgcgacc gccgatgcga ttgataaagc
    13081 agaagaccgc cgtcgtttcg acgtagcgat gaagaaaatt ggtctggaaa ccgcgcgttc
    13141 cggtatcgca cacacgatgg aagaagcgct ggcggttgcc gctgacgtgg gcttcccgtg
    13201 cattattcgc ccatccttta ccatgggcgg tagcggcggc ggtatcgctt ataaccgcga
    13261 agagtttgaa gaaatttgcg cccgcggtct ggatctctcc ccaaccaaag agctgctgat
    13321 tgatgagtcg ctgatcggct ggaaagagta cgagatggaa gtggtgcgtg ataaaaacga
    13381 caactgcatc atcgtctgct ctatcgaaaa ctttgatgcg atgggcatcc ataccggtga
    13441 ctccatcact gtcgcgccag cccaaacgct gaccgacaaa gaatatcaaa tcatgcgtaa
    13501 cgcctcgatg gcggtgctgc gtgaaatcgg cgttgaaacc ggtggttcca atgtccagtt
    13561 tgcggtgaac ccgaaaaacg gtcgcctgat tgttatcgaa atgaacccac gcgtgtcccg
    13621 ttcttcggcg ctggcgtcga aagcgaccgg tttcccgatt gctaaagtgg cggcgaaact
    13681 ggcggtgggt tacaccctcg acgaactgat gaacgacatc actggcggac gtactccggc
    13741 ctccttcgag ccgtccatcg actatgtggt tactaaaatt cctcgcttca acttcgaaaa
    13801 attcgctggt gctaacgacc gtctgaccac tcagatgaaa tcggttggcg aagtgatggc
    13861 gattggtcgc acgcagcagg aatccctgca aaaagcgctg cgcggcctgg aagtcggtgc
    13921 gactggattc gacccgaaag tgagcctcga tgacccggaa gcgttaacca aaatccgtcg
    13981 cgaactgaaa gatgctggcg cagagcgtat ctggtacatc gccgatgcct tccgtgcggg
    14041 cctgtctgtg gacggcgtgt tcaacctgac caatattgac cgctggttcc tggtacagat
    14101 tgaagaactg gtgcgtctgg aagagaaagt agcggaagtg ggcatcactg gcctgaacgc
    14161 tgaattcctg cgccagctga aacgcaaagg ctttgccgat gcgcgtctgg caaaactggc
    14221 gggcgtgcgc gaagcggaaa tccgcaagct gcgcgaccag tatgacctgc acccggttta
    14281 taagcgcgtg gatacctgtg cggcagagtt cgccaccgac accgcttaca tgtactccac
    14341 ttatgaagaa gagtgcgaag cgaatccgtc taccgaccgt gaaaaaatca tggttctcgg
    14401 cggcggcccg aaccgtatcg gtcagggtat cgaattcgac tactgctgtg tacacgcctc
    14461 gctggcgctg cgcgaagacg gttacgaaac cattatggtt aactgtaacc cggaaaccgt
    14521 ctccaccgac tacgacactt ccgatcgcct ctacttcgag ccggtaactc tggaagatgt
    14581 gctggaaatc gtgcgtattg agaagccgaa aggcgttatc gttcagtacg gcggtcagac
    14641 cccgctgaaa ctggcgcgcg cgctggaagc tgctggcgta ccggttatcg gcaccagccc
    14701 ggatgctatc gaccgtgcag aagaccgtga acgcttccag catgcggttg agcgtctgaa
    14761 actgaaacaa ccggcgaacg ccaccgttac cgctattgaa atggcggtag agaaggcgaa
    14821 agagattggc tacccactgg tggtacgtcc gtcttacgtt ctcggcggtc gggcgatgga
    14881 aatcgtctat gacgaagctg acctgcgtcg ctacttccag acggcggtca gcgtgtctaa
    14941 cgatgcgcca gtgttgctgg atcacttcct tgatgacgcg gtagaagttg acgtggatgc
    15001 catctgcgac ggcgaaatgg tgctgattgg cggcatcatg gagcacatcg agcaggcggg
    15061 cgtgcactcc ggtgactccg catgttctct gccagcctac accttaagtc aggaaattca
    15121 ggatgtgatg cgccagcagg tgcagaaact ggccttcgaa ttgcaggtgc gcggtctgat
    15181 gaacgtgcag tttgcggtga aaaacaacga agtctacctg attgaagtta acccgcgtgc
    15241 ggcgcgtacc gttccgttcg tctccaaagc caccggcgta ccgctggcaa aagtggcggc
    15301 gcgtgtgatg gctggcaaat cgctggctga gcagggcgta accaaagaag ttatcccgcc
    15361 gtactactcg gtgaaagaag tggtgctgcc gttcaataaa ttcccgggtg ttgacccgct
    15421 gttagggcca gaaatgcgct ccaccgggga agtgatgggc gtgggccgca ccttcgctga
    15481 agcgtttgcc aaagcgcagc tgggcagcaa ctccaccatg aagaaacacg gtcgtgcgtt
    15541 gctttccgtg cgcgaaggcg ataaagaacg cgtggtggac ctggcggcaa aactgctgaa
    15601 acagggcttc gagctggatg cgacccacgg cacggcgatt gtgctgggcg aagcgggcat
    15661 taatccgcgt ctggtgaaca aggtgcatga aggccgtccg cacattcagg accgcatcaa
    15721 gaatggcgaa tatacctaca tcatcaacac cacttcaggc cgtcgcgcga ttgaagactc
    15781 ccgcgtgatc cgtcgcagtg cgctgcaata taaagtgcat tatgacacca ccctgaacgg
    15841 tggtttcgct accgcgatgg cgctgaatgc cgacgcgacc gaaaaagtaa tttcggtgca
    15901 ggaaatgcac gcgcagatca aataatagcg tgtcatggcg gatatttttc atccgctaat
    15961 ttgatcgaat aagtaatacg gttctctgat gaggaccgtt ttttttgccc attaagtaaa
    16021 tcttttgggg aatcgatatt tttgatgaca taagcaggat ttagctcaca
//
LOCUS       DAESHI010000094        16049 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.095, whole genome shotgun sequence.
ACCESSION   DAESHI010000094 DAESHI010000000
VERSION     DAESHI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16049
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.095"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(36..1859)
                     /gene="typA"
                     /locus_tag="KW011_003106"
     CDS             complement(36..1859)
                     /gene="typA"
                     /locus_tag="KW011_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HBH7661382.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            2232..3641
                     /gene="glnA"
                     /locus_tag="KW011_003107"
     CDS             2232..3641
                     /gene="glnA"
                     /locus_tag="KW011_003107"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HBH7661383.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            3815..4864
                     /gene="glnL"
                     /locus_tag="KW011_003108"
     CDS             3815..4864
                     /gene="glnL"
                     /locus_tag="KW011_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HBH7661384.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            4876..6285
                     /gene="glnG"
                     /locus_tag="KW011_003109"
     CDS             4876..6285
                     /gene="glnG"
                     /locus_tag="KW011_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HBH7661385.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            6397..6507
                     /locus_tag="KW011_003110"
     CDS             6397..6507
                     /locus_tag="KW011_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HBH7661386.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(6697..8070)
                     /gene="hemN"
                     /locus_tag="KW011_003111"
     CDS             complement(6697..8070)
                     /gene="hemN"
                     /locus_tag="KW011_003111"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBH7661387.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8259..8768)
                     /gene="yihI"
                     /locus_tag="KW011_003112"
     CDS             complement(8259..8768)
                     /gene="yihI"
                     /locus_tag="KW011_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HBH7661388.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            9350..9982
                     /locus_tag="KW011_003113"
     CDS             9350..9982
                     /locus_tag="KW011_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBH7661389.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(10364..13150)
                     /gene="polA"
                     /locus_tag="KW011_003114"
     CDS             complement(10364..13150)
                     /gene="polA"
                     /locus_tag="KW011_003114"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBH7661390.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            13514..14446
                     /locus_tag="KW011_003115"
     CDS             13514..14446
                     /locus_tag="KW011_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBH7661391.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(14487..15917)
                     /locus_tag="KW011_003116"
     CDS             complement(14487..15917)
                     /locus_tag="KW011_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBH7661392.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKLLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         3803 a         4097 c         3943 g         4206 t
ORIGIN      
        1 agcgatggca ggttttttat aactaaaaaa cgaaattaat cgtctttcgg tgcgcggttg
       61 gcgcgacgac gatcgttttc cgtcaggtga cgtttacgaa tacggataga ggtcggagtc
      121 acttctacca gttcgtcatc atcgatgaac tccagagctt gttccagagt catgcggata
      181 ggcggaacca gaacaacagc ttcgtcagta ccggaagcac gcatgttggt cagtttctta
      241 ccggtcaggc agtttacagt caggtcgtta gagcggctgt gaataccgat aatctgacct
      301 tcgtaaactt ctgcaccgtg accgaggaac agcttaccgc ggtcctgcag accgaacagt
      361 gcgaacgcga ccgctttacc ctgaccgtta gagatcagta cgccgttctg acgctgaccc
      421 acttcacccg gacgtacgtc gtcgtagtgg ctgaaggtgg agtacagcag accagtaccg
      481 gaagtcatgg tcatgaactc agaacggaag ccaatcagac cacggcttgg gatcacgtag
      541 tcgagacgta cgcggccttt accgtctgga ttcatgtttt tcaggtcgcc tttacgttcg
      601 cccagcgcct gcattacaga accctgatgc tgttcttcaa cgtccagagt cacgttttca
      661 tacggctctt gtttacgacc gtcgatttca cggaagatta cttttggacg ggataccgcc
      721 agttcgaaac cttcacgacg catgttttcg atcagaacag acaggtgcag ttcgccacga
      781 ccagaaacgc ggaacgcatc ggcgtcttcg gtttcttcta cgcgcagcgc aacgttgtgt
      841 accagttctt tgttcagacg atccaggatc tgacgagacg ttacgaactt accttcttta
      901 ccgcagaacg gcgaggtgtt aacgcagaag aacatagaaa cggtcggctc atcaacggag
      961 agtgccggca gcgcttcaac gttttgcgtg tcgcaaacgg tgtcagaaat gttcagttcg
     1021 ccaaggcccg tgatcgccac gatatcgcca gcttccgcca gatcggtttc gatacgttcc
     1081 agaccgaggt ggcccagcac tttaccgact ttcgcgttgc gggttttgcc ttcgctatcg
     1141 atgatagtga cctgctggtt cggcttcact ttaccgcgct tgatgcggcc aatgccgata
     1201 acgccaacat agctgttgta atcgagctga gaaatctgca tctggaacgg accgtcaagg
     1261 tcaacgtccg gcgcaggaac gtggtcaaca atcgcctggt acagcggggt catgtcttcc
     1321 gccatatctt cgtggtccag acccgcgata ccgttcagcg cagaagcgta aacgatcggg
     1381 aagtccagct gctcgtcggt cgcgtcgagg ttaacgaaca gatcgaatac ctgatccaca
     1441 acccaatcag gacgcgcgcc agggcggtca actttgttga taacaacaat cggcttcagg
     1501 ccgtaagcaa acgctttttt ggttacgaag cgcgtttgcg gcatcgggcc gtcaaatgcg
     1561 tcaaccacca gcagcactga gtctaccatg gacattacac gttcaacttc accaccgaag
     1621 tcggcgtgcc ccggggtatc aacgatgttg atacggtaat cattccattt gatagcggtg
     1681 tttttcgcga ggatggtaat cccacgctct ttctccaaat cgttggagtc catcacgcgc
     1741 tcttgggttt cggcacgaga gtcgaacgta ccggattgtt ggagcagctt gtctaccagg
     1801 gtggttttac catggtctac gtgcgcgatg atggcgatat tacgcaattt ttcgatcaca
     1861 actttgcctc aggcattaga aatagcgcgt tattgtacac ggattaatcg cactacaaaa
     1921 caggatcaca aacatcctcc gcaaacaagt attgcagagt ccctttgtga tcgctttcac
     1981 ggagcataaa aagggttatc caaaggtcat tgcaccaaca tggtgcttaa tgtttccatt
     2041 gaagcactat attggtgcaa cattcacatc gtggtgcagc ccttttgcac gatggtgcgc
     2101 atgataacgc cttttagggg caatttaaaa gttggcacag atttcgcttt atctttttta
     2161 cggcgacacg gccaaaataa ttgcagattt cgttaccacg acgaccatga ccaatccagg
     2221 agagttaaag tatgtccgct gaacacgtac tgacgatgct gaacgagcac gaagtgaagt
     2281 ttgttgattt gcgcttcacc gatactaaag gtaaagaaca gcacgtcact atccctgctc
     2341 atcaggtgaa tgctgaattc ttcgaagaag gcaaaatgtt tgacggctcc tcgattggcg
     2401 gctggaaagg cattaacgag tccgacatgg tgctgatgcc agacgcatcc accgcagtga
     2461 ttgacccgtt cttcgccgac tccaccctga ttatccgttg cgacatcctt gaacctggca
     2521 ccctgcaagg ctatgaccgt gacccgcgct ccattgcgaa gcgcgccgaa gattacctgc
     2581 gttccactgg cattgccgac accgtactgt tcgggccaga acctgaattc ttcctgttcg
     2641 atgacatccg tttcggatca tctatctccg gttcccacgt tgctatcgac gatatcgaag
     2701 gcgcatggaa ctcctccacc caatacgaag gtggtaacaa aggtcaccgt ccggcagtga
     2761 aaggcggtta cttcccggtt ccaccggtag actcggctca ggatattcgt tctgaaatgt
     2821 gtctggtgat ggaacagatg ggtctggtgg ttgaagccca tcaccacgaa gtagcgactg
     2881 ctggtcagaa cgaagtggct acccgcttca ataccatgac caaaaaagct gacgaaattc
     2941 agatctacaa atatgttgtg cacaacgtag cgcaccgctt cggtaaaacc gcgaccttta
     3001 tgccaaaacc gatgttcggt gataacggct ccggtatgca ctgccacatg tctctgtcta
     3061 aaaacggcgt taacctgttc gcaggcgaca aatacgcagg tctgtctgag caggcgctgt
     3121 actacattgg cggcgtaatc aaacacgcta aagcgattaa cgccctggca aacccgacca
     3181 ccaactctta taagcgtctg gtcccgggct atgaagcacc ggtaatgctg gcttactctg
     3241 cgcgtaaccg ttctgcgtct atccgtattc cggtggtttc ttctccgaaa gcacgtcgta
     3301 tcgaagtacg tttcccggat ccggcagcta acccgtacct gtgctttgct gccctgctga
     3361 tggccggtct tgatggtatc aagaacaaga tccatccggg cgaagccatg gacaaaaacc
     3421 tgtatgacct gccgccagaa gaagcgaaag agatcccaca ggttgcaggc tctctggaag
     3481 aagcactgaa cgaactggat ctggaccgcg agttcctgaa agccggtggc gtgttcactg
     3541 acgaagcaat tgatgcgtac atcgctctac gtcgcgaaga agatgaccgc gtgcgtatga
     3601 ctccgcatcc ggtagagttt gagctgtact acagcgtcta atagttgaag ttgtactacc
     3661 cggcgcaaca acgccgggat ttagttgccg tggaaacttt cagcccatct ctgcatgggc
     3721 ttttttctcc gtcaattctc tgatgcttcg cgctttttat ccgtaaaaag ctataatgca
     3781 ctaaaatggt gcaacctgtt caggagactg ctttatggca acaggcacgc agcccgatgc
     3841 tgggcagatc ctcaactcgc tgattaacag tattttgtta attgatgaca atctggcgat
     3901 ccattacgcc aaccccgccg cgcaacaact gctcgcccaa agctcccgca aattgtttgg
     3961 tacaccgtta ccggaactgt tgagctactt ctcattaaat atcgagctga tgcaagaaag
     4021 tctggaggcg gggcaaggtt ttaccgataa cgaagtgacg ctggtcatcg acgggcgctc
     4081 gcatatcctt tctgtgacgg cccagcgtat gccggacggc atgatcctgc tggagatggc
     4141 tccgatggat aaccagcgcc gcttaagtca ggaacagcta cagcacgccc agcaggttgc
     4201 tgcccgtgat ttagtgcgcg gcctggcaca tgagattaaa aatccgcttg gaggtttacg
     4261 tggcgcggcg cagctgctca gcaaagcgtt acctgaccca tcactactcg aatataccaa
     4321 agtgattatc gaacaggcgg accggctgcg aaatctggtc gaccgtctgt tggggccgca
     4381 gctgcccggt acgcgcgtta ccgaaagtat tcacaaagta gctgaacgcg tggtgacgct
     4441 ggtgtcgatg gaactgccgg acaacgtgcg gttgattcgt gattacgacc ccagcctgcc
     4501 ggaactggcg cacgacccgg atcaaattga acaggtcttg ctgaatattg tgcgcaatgc
     4561 gctacaggcg ctggggccgg aaggcggtga aatcattctg cgtacccgca ccgcgtttca
     4621 actgacctta cacggcgagc gctaccggct ggcggcgcgg attgatgtgg aagataacgg
     4681 gccgggcatt ccgcctcatt tgcaggatac gctgttttac ccgatggtca gcggccgcga
     4741 aggtggcacc gggcttggct tatccatcgc ccgtaatttg attgatcagc attcaggcaa
     4801 aattgaattt accagttggc caggtcatac cgagttctcg gtttacctgc ctatcaggaa
     4861 ataaaggtga cgtttatgca acgagggata gtctgggtag tcgatgacga tagttccatc
     4921 cgttgggtgc ttgaacgtgc gctcgctgga gcgggtttaa cctgtacgac atttgagaac
     4981 ggcgcggaag tactggaggc gctggcgagc aaaacgccgg atgtgctgct ttcagatatc
     5041 cgtatgccgg gaatggacgg gctggcgctg ctcaagcaga ttaaacagcg ccatccaatg
     5101 cttccggtca tcattatgac cgcacattcc gatctggatg ctgccgtcag cgcctatcaa
     5161 caaggggcgt ttgattatct gcccaaaccg tttgatatcg acgaagcagt ggcgctggtt
     5221 gagcgcgcta tcagtcatta ccaggaacag cagcagccgc gtaatattca gcttaacggc
     5281 ccaacgaccg atatcatcgg cgaagcgcca gccatgcagg acgtgttccg tattatcggt
     5341 cggctttcgc gttcttctat tagcgtgctg attaacggcg aatccggcac cggtaaagaa
     5401 ctggtcgctc atgccctgca tcgccacagt ccgcgcgcca aagcgccgtt tatcgcgctg
     5461 aatatggcag ctatcccaaa agatttgatc gaatcagaac tgtttggcca cgagaaaggc
     5521 gcgtttactg gcgcgaatac cattcgtcag gggcgttttg aacaggccga tggcggtaca
     5581 ttattcctcg acgaaattgg tgatatgccg ctggatgtgc agacgcgttt gctgcgcgtg
     5641 ctggcagacg gtcagtttta ccgcgttggc ggctatgcgc cggtgaaagt ggatgtgcgt
     5701 attatcgctg ccactcacca gaatctcgaa caacgagtgc aggaaggtaa gttccgtgag
     5761 gatctgttcc accgcctgaa cgttatccgc gttcatctgc cgccgctgcg cgaacgtcgg
     5821 gaagatattc cccgtctggc gcgccatttt ttacaggttg ccgcgcgcga actgggcgta
     5881 gaagcgaagt tactgcatcc ggaaaccgaa gctgctctga cgcgtctggc gtggccaggc
     5941 aacgtgcgcc agctggaaaa cacctgccgc tggctaacgg tgatggccgc cgggcaggaa
     6001 gtgttgattc aggatttgcc cggcgaactg tttgaatcaa cggttgcgga gagtacttcg
     6061 caaatgcaac cggacagctg ggcaacgctt cttgcgcagt gggcagacag agcgctgcgt
     6121 tccggtcatc aaaatctgct ttccgaagcg cagccagagc tggagcggac gttactgacg
     6181 accgcgttgc gacatacgca ggggcataaa caggaagcgg cgcggctact cggctggggc
     6241 cgcaacaccc tgacgcgtaa gttaaaagag ctggggatgg agtgattcac agcttgtgtg
     6301 taaagattga ttattgagcg caaattgctg gtattttacg ctttactgtt ccgataagtt
     6361 cagtatgatc ttgcccggaa aacggggaga gtcattatgc tggaatcaat aattaatctg
     6421 gtatcgagtg gcgcagttga cagccacaca ccgcaaactg ccgttgctgc cgtgctgtgt
     6481 gccgcaatga ttgggctgtt tagttgagtt tcagattgct gatgaagtgc tctttgttca
     6541 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag caatattttc aacaggtttt
     6601 gtaggcctga taagcgtagc gcatcaggca atcttgttct cagcacattg atggcgcttt
     6661 cgttttctac tttgtaaacg aagcgccatt cactatttaa atcacccgtg agaactgctg
     6721 catccgcgct ttctggcgca gataggtatc aaagcacatg caaatgttgc ggatcagcaa
     6781 gcgacctttc gccgtcacct gtattccctt ctcatccaca tccaccagcc catcttttgc
     6841 taacggggcg agcagcttga gatcttccgc aaagtaatca gcgaagtgca aatcccactg
     6901 tttctcaata ggggcgtaat ccagacggaa gttgcagatg agcgacttaa tcacatcgcg
     6961 gcgaatacag tcatcacgcg ttagcgcaat accacgccac agcgcattgc cttgttcatc
     7021 cacttgctga tagtactgct tcaactcttt ctggttctgc gcgtagcagt cgccaatcat
     7081 gctgatggcg gaaacgccca tccccagcag atcggtatcg ccctgagtgg tgtagccctg
     7141 gaagttacga tgcagcacgc cttcacgctg ggccaccgcc agctcgtcat ccggacgggc
     7201 aaagtgatcc ataccgataa actgatagcc cgattgcgtc aggaaggcga tggtttcctg
     7261 caggatatcg agtttttgct gcggactcgg caggtcagca tctttgattt tgcgctgagc
     7321 agcaaaaatg gtcggcagat gcgcgtagtt aaagacactc agacgatcgg ggttcagctc
     7381 cgccacacgt ttcagggtaa aggcgaaact ctccggcgtc tgtttcggca ggccgtaaat
     7441 caggtcgatg ttggtggagg taaaaccaat ctcacgcgca tggttaagca gtgcaaagat
     7501 gaactcttca tcctgctcgc ggttaaccag acgttgcact tctttgttga agtcctgcac
     7561 gcccatgctc aggcgattaa agccttcggc gcgtaaatga tcgagtacat ccagttcgat
     7621 ttcccgcgga tcgacttcga tcgaaatctc cgcatcggca ttgaactgga agttttcgcg
     7681 cagcagcttc atcaggcggc tgatttgcgc tttattcaga tacgtcggcg ttccgccgcc
     7741 ccagtgcaat tggctgacgt gacgcccggc gaacagcggt gcacgatgga cgatttcttg
     7801 ctccagcgcg tccagatact gatcggcctt gtgctgctgg cgagtaacaa tcttattgca
     7861 accgcagaag taacaaagct tatggcagaa cgggatatgt acgtagagag ataatggacg
     7921 ctcaggatag cgcgccacgg cttgtaaaaa cgcctgttcg ccgaagtctt ctgaaaactc
     7981 cagcgcggtc gggtacgagg tgtatcgtgg cccggaatag ttatatttct ggatcagggc
     8041 cagatcccag tcgatttgct gtacagacat gctcactcct tccgatggcg tctctggcgg
     8101 ctacggcgta ccggcggatg cccgtttcgg gtcatcagcc ggttgcgcag ccacttttgt
     8161 cgccgcgaca accgctgtag tttaacgaat aaccacacca gataacatat aaccggaagg
     8221 gttatcagca gaacgggaag gcccacggtg gcaatccgtt agttaccccg cagcagacgc
     8281 atcatgtctt cttgcttctc gtcttcttcc tcttcttcgt catcgtcata agagaggccg
     8341 agtttctgca tcaactcatc aatacgatcc agtttagcat ccacccagga ttgctcttcg
     8401 gcgctcaggg tttcgcctgc ttccagacgt tccagcagcg catccagacg ctcatccgtt
     8461 tccagtaatt ccaactccgc ctgcggtgaa agcataggtt tctcactctt cggtttgtgc
     8521 tgtttggtga ctttttcagc cacgcccaat ggaatagggg ttttactgcc aatacgtgga
     8581 tcttttggtg cgttctggcc tttgctgcct gacgtggtgt ttccgcccgc tgcgcggctg
     8641 cccggcgcat gaccacgacg ttttttctga cgcttgcgat cacgagcttc ctgatccagc
     8701 tcctcgcgtg tttttcgacg cgcttttgca tgacctttgc tgcgtgagtt tgaagatgat
     8761 ggtttcataa tggttcgtct tttagccgtt ttattcagta tagatttgcg gcggaatcta
     8821 acagaaagca agcaaagaaa aaaggcgaca gattactctg tcgccttttt tcctgactca
     8881 taacccttaa cgggtcttac aatccttgca ggccgccaac acaccctgtt tttcctttag
     8941 ccggaacaat ccgtgttgat tccatttccg tttaattacg tctccggacg tttgtcttcc
     9001 tgacaatcct ctgtcttcgc ctcctggcgc tcctgaccct catcctgagt cattgttcct
     9061 gttagcgtcc tcgctctatg ggtgctactt tacgcctttg ctttaaacaa acaatcaacc
     9121 gccatcagta tttgcacatt ttcagagttt tatataaaag caattcactg atttatatag
     9181 cttatttgac atgcatcgca gaattttctc tgctatttcg cataaagact atcggcaatg
     9241 tctcacaaag cgtatggctt ttacttacaa cgctcacgcc gaaaaatccg ccttttatta
     9301 tggttcaggt ataatcgcca caaagctttg actgacggag acgaccgctt tgactaattt
     9361 gaattatcaa cagacgcatt ttgtgatgag tgcgcctgat attcgccacc taccttccga
     9421 taccggaatt gaagtggctt ttgcaggccg ttccaacgca ggtaaatcca gcgcgctgaa
     9481 cacgctgact aaccagaaaa gcctggctcg tacctcaaaa accccagggc gcacccagct
     9541 tatcaacctg tttgaagtgg ctgacggcaa gcgtctggtt gacttgcctg ggtacggtta
     9601 tgcggaagtc ccggaagaga tgaagcgcaa atggcagcgt gcgctcggcg aatacctcga
     9661 aaaacgtcag agcctgcaag gtctggtggt gctaatggat attcgccatc cgctgaaaga
     9721 tttggatcag cagatgattg agtgggcggt agacagcaat atcgccgttc tggtgctgct
     9781 gaccaaagcg gacaaactgg caagcggcgc acgtaaagcg caattgaata tggtgcgtga
     9841 agctgtactg gcgtttaacg gtgatgtgca ggttgaaacg ttttcttcgt tgaagaaaca
     9901 aggcgtggac aagctgcggc agaaactgga tacctggttt agcgagatgc agcctgtaga
     9961 agaaacgcag gacggcgaat aattttcttg ccttaatgct tgtgccggat gtggcgtatc
    10021 cggcccgtaa attcaataga ttacaattct accgttggcc tgataaacat agcgcatcag
    10081 gcattacgga tcttttcttt cgcccaataa aaaacgcccc agtcattact gactggggcg
    10141 gctaaaatat tcagccaaat ccgattacgt gaagtaaaag gtctgaaaga tagaacatct
    10201 tacctctgta ccctacgcga ctaactttac tcttttttgt tcagttcaga aagcactttt
    10261 tgtaattttt tttcagttgt tgcataggaa tttctaatag ccatcacaaa acgtgacagc
    10321 ttatgttgct tacttacgaa aaaagggcat gttcaggcga atcttagtgc gcctgatccc
    10381 agttttcgcc actccccact tccaccagca acggcacatc cagacgggta cagttttcca
    10441 tcagttgatg aatctgcttc gcgacggcat caacatcatc tttatgaact tcaaatacca
    10501 gttcatcgtg tacctgcatg atcatacgta cacgcggttg ctcagcctgt aaccacgcat
    10561 caacggcaat catcgcccgt ttgataatgt cggcggcggt tccctgcatt ggcgcgttaa
    10621 tggctgcacg ttcagccgct gcacgacgag caccattgct ggatttgata tccggcagat
    10681 acagacggcg tccgtccagc gtttcaacgt agccctgctc tttcgcctga gcacgggtgc
    10741 gttccatata ctccagcacg ccagggtagc gttcgaagta aaggtccatg tacttctgcg
    10801 cttctttacg tggaatgttc aattgccgcg ccagaccgaa agcactcatg ccataaatca
    10861 gaccaaagtt gatcgctttc gcgctacggc gttgctcgct ggtgacggtt tccagtggca
    10921 aaccaaacac ttctgccgcc gttgcccggt ggatatcttt tccttccgcg aatgcggtca
    10981 gcaagccttt gtcacgcgaa agatgcgcca taatgcgcag ttcaatctgc gagtagtccg
    11041 ctgagacaat cacataatcc tctggcgcaa taaacgcctg gcggatacga cgaccttctt
    11101 cgttacgcac cggaatgttt tgcaggttag gatcggttga cgataaacgt cccgttgcag
    11161 ttactgcctg gtgataagag gtatgcacac gcccggtttt cgggttgatc atcagcggca
    11221 gcttgtcggt gtaggtcgat ttcagcttcg ccagaccacg atactccaga atcacttttg
    11281 gcaacggata gtccagcgcc agttcttcca gtacctcttc cgacgttgac ggcgcgccac
    11341 ccggcgtttt cttcagcggt ttaatgccct gtttttcaaa cagaatggtt tgtaactgct
    11401 tggtggaaga aaggttaaat tcctcacctg caatttcatg cgctttcttt tccagctcag
    11461 ccagacgaag ggtgagctct tcagaatgat tgtgcagcac tttcggatcg atcttcacac
    11521 cgttacgttc aatgcgtgaa agcaccggca ccagcggcat ttcgatattc tcgaagacgt
    11581 tcaacggccc tttgtgtttt tgcagatctg gccacatttt cagatgcaac tgcaaggtga
    11641 catccgcatc ttcggcggcg taacgtccgg cctcttcgag ggcaatctgg ttaaaggtca
    11701 gttgattttt gcctttaccc gcaatctctt caaaagtgat agttttgtgc ttcaaccaac
    11761 gttccgcgag gctgtccata tcgtgacgcc cggcaacgct attgagaatg taggactcca
    11821 gcatggtatc aaacgcaatc ccacgcagct caatgccgta attcgccaga ataccgcgat
    11881 cgtatttcag gttttgcccg accttcagcg ccttttcatc ttccagcagc ggttttagca
    11941 actcgagtgc acgctcgcga gagatttgat cgggcgcatc aagataatca tgagcaaccg
    12001 gaatatatgc cgctacgcct ggctcgatag caaaagaaag cccgaccagg ttagcagaga
    12061 tgttatcaag gctgtcggtt tcggtatcaa atgcaaatac cggcgctttt tccagcttcg
    12121 caatccacgc tttcagtgtt tcttcatcaa ggatggtgac gtagttgtca taagaaatca
    12181 ccgttgccgt cacttctggc gcttcgtctg caacactggt ttcctgcggc ttcgcggctg
    12241 gttttgcccc tttggcctgt aaccatttgc ccgcttcgac atcagcagtc cagcgtttga
    12301 actcatactt tttgaacagc cccaacaact cttccgctgc cggttgctgc acttccagtt
    12361 gttcacaggt cagctccagt tcaacgtcgg ttttaatcgt cgccagctgg tatgagagat
    12421 aagcaacttc tttgttttgc tcgagcttcg ctgccattgt tttcgcgcca cggaagctca
    12481 acccagcaat tttttctggc tcggcataca gcgtatccag cccgccaaga ccttgcagca
    12541 atgcctgcgc ggttttttca ccgacgcccg gtacgccagg aatgttatcg gaggagtcac
    12601 ccatcagcgc caggaaatcg atgatcagtt ctggcggcac gccgtactta ttcaccacct
    12661 cttccggtcc gaggatggta ttcgtcatgg tgttgataag cgtaatattt ggcgtcacca
    12721 gctgcgccat atctttatcg ccagtgctga tcagcaccgg acgcccggct ttttcggctt
    12781 cgcgcgccag agtaccgata acgtcgtccg cttctacgcc agaaaccgcc agcagcggca
    12841 gtcccatcgc tttaaccatc gcgtgcaggg gttcgatttg tgctcgcaga tcgtccggca
    12901 ttggcgggcg atgtgatttg taatgttcaa acagttcatc acgaaaggtt tttcccttgg
    12961 cgtcaaagac caccgctgca tgcgtcggtt tatattgcat gatcagactg cgcagcatgt
    13021 tgaggacacc atacatcgca ccggtcggct cgcctgcgct gttagtcagc gggggaaacg
    13081 cgtgatatgc gcgataaaga taagatgaac catctacaag gataagtgga ttttggggga
    13141 tctgaaccat aatgtccgtg cctgtttatc agattatggg taaaggatgc cacagacagg
    13201 atgaaaacat gagtttttca ccgcttttcg tgaaaatctt ttgtagatct tctggatcgc
    13261 tcgcaaatcg tcatgtggat aactttgtgc ataaattatt tacatcgctt ttaatttatt
    13321 atcatgcttt ttacagcatg atgtttgtct ttgtttttca tgcagttaaa aataaagcca
    13381 tccctaataa ttgagataat gggatttttt ctaatgtgga tagatatgga ttatttttct
    13441 ccttaaggat catccgttat ttgggtcgtt ttctgctaaa atctgcgcct tgcgcttctt
    13501 tagcgcactg tctatggcta accttttgaa taaattcatt atgacgagaa tactcgctgc
    13561 gataaccctt ttgctgagta tcgtattgac cattttggtc actatttttt gttccgtccc
    13621 gatcatcatt gccgggattg taaaactatt gctacctgtg ccagtcattt ggcgaaaggt
    13681 ttcgcgtttt tgtgatttta tgatgtattg ctggtgtgaa ggtctggcgg tattactgca
    13741 tctgaaccca cacctgcaat gggaagttca cgggctggaa gggttaagta agaagaactg
    13801 gtatctgctt atttgtaacc accgtagttg ggcagatatt gtcgtactgt gcgtgctgtt
    13861 tcgtaagcac attccgatga ataaatattt cctcaagcaa cagctggcct gggtgccatt
    13921 tcttggcctg gcgtgctggg cgctggatat gccgtttatg aagcgttatt cccgcgctta
    13981 tttattacgt catccggagc gacgtggtaa agatgttgaa accactcggc gttcttgtga
    14041 aaagtttcgc ctgcatccca ccaccattgt caatttcgtt gaaggctccc gtttcacgca
    14101 agaaaaacat cagcaaaccc actccacttt tcaaaacttg ttgccaccaa aggcggcagg
    14161 cattgcgatg gcgctaaatg tactgggtaa acaattcgat aaactgctta atgttacgct
    14221 gtgttatccg gacaataacc gtcagccgtt cttcgatatg ttaagcggta aactgacgcg
    14281 gattgtcgta catgtggatt tacagcctat tgccgatgag ttacatggcg actacatcaa
    14341 tgacaaaagc tttaagcgcc attttcagca atggctgaac tcattgtggc aggaaaaaga
    14401 ccggcttctg acgtctctca tgtcatcaca gcgtcagaat aagtaagttt tactcgcctc
    14461 tgaatgagca gaggcgagtg agtattttaa tgaacaaaac gtccggcacg agacataaat
    14521 tcttcttcgc tcatctcctg tccgttaaac acaactttac ccggtgtata acgcagctgc
    14581 agcgaggcgg tattgttgtc aatcgtgatt aactgaaaca tctgacccaa tgtcatcatc
    14641 ccgctgattt gtttatcagc ctgcttttgc gctttttccg catccattcc ttcagataaa
    14701 ttaagctgtt ttgcggtttc tgtgacgaca ttaagcggta acttcacatc aaaattgagc
    14761 aatttgatat ctttgtttgt ggatgatgaa gatttggctg ggtcggcaat actgatatcc
    14821 agattggcat ttagttcgcc gagtgcgttc ttccatctta ccggttgttt aatggtcggc
    14881 tcactttttt gtaataacgg taaatactct ttgaacaatg cagcattcac ttcttgcaga
    14941 gcaacttcat cgtttgctaa ctcagggtgt acagcaagct gcttctgcat agcaatgtta
    15001 tactggatga taaattggcg cactgctgag ggatcaatag attcagcaat caatgaccac
    15061 tcaccactac cgagttgctg attccctttt gttaacttat caagttcgta ggtcagattg
    15121 acattgatat aatctttgtc tttaacgcga tccagcatgg tttttgcaac gaaagcatcg
    15181 atttgggcaa cgtcttcccc gtgattgata atgttaatct gattaaattt cgactcgctt
    15241 tcaccaaccg gaaatttctt ttcttctaat cgcgccagag aatcaatggt aaaacttttt
    15301 gccgttactt tgttcattgg tgataattgc agaacaatat cttcgacttt gccttcaata
    15361 tccaggttag acaatgaact accatcacca ttaaaagtaa actggccctc accaaaagaa
    15421 aactttccat actctgcagg ggcaacatct aatgtcgtag agaatgagcc accaaacgcg
    15481 aatcgcaatg tcgcttccac gggcgattta tttttcgcgg cgataaatag cggttgtgtc
    15541 agttcattat tcactaagtt cacttttgcc gctgccagtg ctgggatgac attaccatgc
    15601 attaacattg tgatgggcaa cggaccgtgc tcaacatcca catcaaatac aactttctgg
    15661 cctggcttga tattgagatc gggtgcaccg ttatcgaatg tcatttgcat ttgaaaacga
    15721 gaattaaaaa aacctttgtc aaaattttta taacttaacg tcatatcgta ggcatgttca
    15781 cctttctttt tagcatcgtt aaaatcttta ataaactttt cgacaccagg ctgaatttgc
    15841 gtacctgtgt accatgtgcc accaccccag attacggcta acgcaacaat aacacctgta
    15901 gctgacttac gtatcatata aatattccct gataaaacag caaaacttta aatatccgct
    15961 tatgctgaaa taatgtccag cggcaggacg cattatcaga atgtatttat atttattaaa
    16021 taataaaaaa aaccgtgaat attcacggg
//
LOCUS       DAESHI010000095        15961 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.096, whole genome shotgun sequence.
ACCESSION   DAESHI010000095 DAESHI010000000
VERSION     DAESHI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.096"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..453)
                     /gene="spy"
                     /locus_tag="KW011_003117"
     CDS             complement(<1..453)
                     /gene="spy"
                     /locus_tag="KW011_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HBH7661393.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPA"
     gene            complement(783..1751)
                     /gene="astE"
                     /locus_tag="KW011_003118"
     CDS             complement(783..1751)
                     /gene="astE"
                     /locus_tag="KW011_003118"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HBH7661394.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1744..3087)
                     /gene="astB"
                     /locus_tag="KW011_003119"
     CDS             complement(1744..3087)
                     /gene="astB"
                     /locus_tag="KW011_003119"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HBH7661395.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(3084..4562)
                     /gene="astD"
                     /locus_tag="KW011_003120"
                     /pseudo
     CDS             complement(3084..4562)
                     /gene="astD"
                     /locus_tag="KW011_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(4559..5593)
                     /gene="astA"
                     /locus_tag="KW011_003121"
     CDS             complement(4559..5593)
                     /gene="astA"
                     /locus_tag="KW011_003121"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HBH7661396.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(5590..6810)
                     /gene="astC"
                     /locus_tag="KW011_003122"
     CDS             complement(5590..6810)
                     /gene="astC"
                     /locus_tag="KW011_003122"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HBH7661397.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            7256..8062
                     /gene="xthA"
                     /locus_tag="KW011_003123"
     CDS             7256..8062
                     /gene="xthA"
                     /locus_tag="KW011_003123"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBH7661398.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            8229..8939
                     /locus_tag="KW011_003124"
     CDS             8229..8939
                     /locus_tag="KW011_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBH7661399.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8944..9621
                     /gene="ydjY"
                     /locus_tag="KW011_003125"
     CDS             8944..9621
                     /gene="ydjY"
                     /locus_tag="KW011_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HBH7661400.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            9636..10343
                     /locus_tag="KW011_003126"
     CDS             9636..10343
                     /locus_tag="KW011_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBH7661401.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            10343..10891
                     /locus_tag="KW011_003127"
     CDS             10343..10891
                     /locus_tag="KW011_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBH7661402.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10901..12067
                     /locus_tag="KW011_003128"
     CDS             10901..12067
                     /locus_tag="KW011_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH7661403.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            12040..13576
                     /locus_tag="KW011_003129"
                     /pseudo
     CDS             12040..13576
                     /locus_tag="KW011_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            13576..14229
                     /locus_tag="KW011_003130"
     CDS             13576..14229
                     /locus_tag="KW011_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH7661404.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            14296..15603
                     /gene="ynjE"
                     /locus_tag="KW011_003131"
     CDS             14296..15603
                     /gene="ynjE"
                     /locus_tag="KW011_003131"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HBH7661405.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(15612..>15961)
                     /locus_tag="KW011_003132"
     CDS             complement(15612..>15961)
                     /locus_tag="KW011_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBH7661406.1"
                     /translation="FYALVPFGFILAAPEQNALAGGRLLFAFIGTGSSFLAFAALAAK
                     HQIDNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRV
                     WSGYLTLKSLQRQ"
BASE COUNT         3698 a         4344 c         4165 g         3754 t
ORIGIN      
        1 cgctggacgt tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg
       61 cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg
      121 ctgttcttcc atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc
      181 aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg
      241 gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa
      301 catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc
      361 cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt
      421 agaggcaaca aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt
      481 attacggctt tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt
      541 caattaatca gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat
      601 ggagaaaaaa agaaaatttg atggagagtg atgagagaat attacaacac aatgattttg
      661 cagagattat gaagaactat accggatgac tggtgataaa taaagcaaat aaccaggatt
      721 aatctgtatt aatttataag aaagcaactt aatacccgca gaatgatttc tgcgggtaag
      781 tattagctta ttttttcgag cattaatccc gcgcgtaatc ccaacgctac caacggatta
      841 gggaataaca catactctac atcatgggtt acggtaaaac gttcctctcc gtcctgcgcc
      901 agcaatgttc ctttctcaaa cggcataaaa ttcagcgtgt cacttgccat atgcatttcg
      961 aaggacggcg agtggcgagt aatttgcgaa accacccgat aacggagcgg cggtgttctc
     1021 acgataccga cactctcacc agatagcagc gcagcaattg cgctggcagt tactgcaaac
     1081 tggcgaagat cgttttgccc aaagggcaac gctttgccaa gttccagcgt acaggccagc
     1141 gcgccaaaat gtctggcgct gaaatgggta aacgtaccac caggctcctg atggaacacc
     1201 agcgcctcca gccccgccgc acccagccac gtcagaaatt tctcgtccca gggaatgtcg
     1261 cgttgcggta atacaccgaa ctgaggatgc aaggagccac gaattgcggt atgtagatca
     1321 aggtgccaac gcacagattc tttgccctgg tcataaaaat cttccaggca ctgttccagt
     1381 tcgcgcgccc gacaggtttc tccgctttca gcaaatagct gccaacgacc gccaaacatt
     1441 cggttcatat cgctatggca ataacgtttc ccttgcttca gcgcaggagg atccccgagg
     1501 atcaccagca accgccaacg taacgggatc tcgccgtgag atatcgcgcc aagcaacgcg
     1561 tccagcattt ccacaggtgc cgtctcatta ccgtggatcc ccgcagaaat caccagcgcg
     1621 ccttgcggtg gcgttaatgg cgtcagttcc agcacaccat cgcccagcca gcgccagcga
     1681 acgccgttga tttctcgctc ggtgataacc ggttttttac ccgttaaggt cagagcaaga
     1741 aaattatcca ttgccccctc cctcgcgctg gaacggataa accgaaccga gattcagtaa
     1801 ttggctcaat acatccagtg cttcccgccc ttcgcgcagc aattgcgggt cggccagatc
     1861 ggcagcagta aggcgatcgc ggtagtaacg atccacccag tcattgagcg cattaaacag
     1921 cgtatcgttc atcatcaccg ccggattcac cgcccggcgt tcttcttctg tcaatactac
     1981 ccgcaaccgc aggcacgccg ggccgccgcc attcgccatg ctttcacgta aatcaaagac
     2041 ttttagttcg ctaatcgggt tgtcagcggc aaggagttca ttgagataac cccatactcc
     2101 ggcgtgttcc cgacactcct gaggcagcac caacatcatg gaaccatcat cgcggctcaa
     2161 cagttggctg ttaaacagat aggtagacac cgcatccgac acggaaacct gagttgccgg
     2221 aacttctatc gccataaaac cgttcacccg cgcccgcagg tttgccagta actgtgactg
     2281 gcgagcgaac gcctgttggt ggcaaaacag cacctggcgg ttacttacgg caatcacgtc
     2341 attatgaaaa acaccctggt cgataacgtc cgggttttgc tgggcgaaaa tcacctgttg
     2401 gggattcacc tgattaagcc ttgccaccgc ctcgctggct tcgcgagtct gtcgcgccgg
     2461 ataacgggaa ggccgggtat cattgccttc ttctcgcccg tagacaaaaa gttgcatacc
     2521 cggctcaccg taatgaccgc cgagacgatt gtggtttgcc gccccctcat caccgagcaa
     2581 cgctacctgc ggcaacgccg aatggacgct aaatttctct tcgtcgttaa aaatagcttt
     2641 taacagcgat tcagtgacgg gcgcttccag cgaacggtga aatttattgt tcaggttggc
     2701 aacggtgaga tgcactttgc catccagcgt atcggcagat ggcgcgatcg ttgccgcatt
     2761 ggctacccac attggcgaag cggagctgac gctggaaagc cagtgcggtg cctggcgtgc
     2821 aactttttcc agtacctgct catcgctacc actgaatccc aactgacgca gcaccggaat
     2881 aaacggacgc tcgtgcggcg ggatcacggc ctgggggaat cccgcatcgg caagggtttt
     2941 cattttcagt aagccctgtt tcgccgccag tcgcgggtta gacacctgaa aacggtgacg
     3001 ggtagaggct tcattaccaa acgacaggcc agcgtaatga tgcgtcagcc ctaccagccc
     3061 gtcgaaatta acttcccagg cgttcatcgc accacctcat cggaaaaatc cagcccgggg
     3121 ttaagcgttg cgggcaaagt taacgagtcc gactccaggc tcgccatagg ccatgcgcag
     3181 taatctgcgg cataccaggc gctggggcga tggttaccgg atgcaccaat gccgccgaat
     3241 ggcgcggtac tggcagcacc agtaagcggt ttgttccagt taacaatccc cacccgcgcc
     3301 tccagcaaca gttgatcgaa tttttcccgc tcgggggaaa ccagaccgca agagagtccg
     3361 aagcgagtgt tattcgccat tagaatcgct tcctcgaaag aatcataacg ccagacgcgc
     3421 aataacggtc cgaacacctc ttcatctggt acgccagcaa cgcctgtcat ttcaatgatc
     3481 cccggcgtca gcaacgatgt ctctgattgt aataagcgcg gcgcaagcag ggttcgtccg
     3541 cccatcgctt ccagttgctg ctaggcagta accacctgct gtgcggcctg ttcagaaatc
     3601 agcccgccaa taaacggctg cggttcgtca tcccagttgc ccggcgttaa tcgctggctg
     3661 acggcaacca gacgagcaag aaacgcatcg ccctgtgctc cgcttttgag caataaacgg
     3721 cgggcgcagg tgcagcgttg cccggctgtg acaaacgccg actgaatggt cagatggaca
     3781 gccgagtcga tatccgccac ctcatcgata attagcggat tattaccgcc catctcaagg
     3841 gcgagaattt tctccggctg accggagagc tggcgatgca gctggtagcc ggtattggcg
     3901 ctaccggtaa acagcaaacc gtcgagatcc tccagcgcac tcagcgcctg acccgtttca
     3961 cgcccgccct gcaccaggtt cagcacgccc ggcggcaagc cagcctgctg ccataaacgc
     4021 attaccgctt cgccactcca cggtgtcagt tcgctgggtt taaagataat ggtgttacct
     4081 gccagcaatg ccggaacgat atgtccgttc ggcaaatgac cagggaaatt atacggccca
     4141 aacaccgcca gcacgccgtg cgggcgatgt cgcaggctcg ccgcaccgtc cggcatttca
     4201 ctacgctgct cgccggtacg aacgtgatac gccttaattg atatcgcgat tttattgatc
     4261 atcgccgtca cttcggttgc cgcttcccag cgcggcttac ccgtttctct ggcaataatc
     4321 gcggttaatt cggctttatt gctttccagc aatccggcaa agcgttcgac aacgacctga
     4381 cgttcagcca atgagagccg cgcccagcgc ggaaacgccg cacgggctgc ccgacaagcc
     4441 tgttcgacct gagcggcatc ggcatcattg ccttgccata acacctcgcc cgataccgga
     4501 ttacgcttca cacgcgatgc gccctggccc gttatccagt caccgttaat ccataaagtc
     4561 atgctgtttt ctcctctgcg cacaggcgca ccagacgaac gcgatcccct gcgtggcatt
     4621 tgagggcatc cagttgtgcg gcggttaaaa tcaaacgctc ggttgccgga tcggtacgcg
     4681 ccagcaccac gcggaaatgg tgataatttt cattggcgac caggcaggcc ggaaaatcgc
     4741 cctgcgcagg ctgcccttct gccacttcca ccagccgact tttacggatg gcgcgcacgc
     4801 ggtcgatgtc acactcaagc gttggcccgc cgtcgaagat gtcgatatag ttgcggtaac
     4861 gaaaaccttc tttctccagc accgcgcggg caggcgcggt ttgcggatgt acctgaccga
     4921 tgacgtcctg ggcttcctgg gataaaaagt gggtatagat cggatgtttc ggcatcagtt
     4981 ctgcaataaa tgccttttgc ccggtgccgc agagaaaatc gacgcggcta aaatccatcg
     5041 aaaagaagcg tttaccgagg ctttgccaga acggtgaata gccgtgttcg tcaatcaccc
     5101 cgcgcatttc agcaaccact ttgtcattaa acttgtcgcg aaaagccgcc ataaacataa
     5161 agcgcgattt cgacagcaaa tagccgttgc cctctttgcg ccagtccggg tcgagaaaca
     5221 gcgtgcacag ctcgctgctg ccggtgtgat cgttactaag aaacagcgtc ggcaatgcgt
     5281 tatagacatt cagctctttt gaggcgtgaa ccaacgtgcc gacccgatag ttgtaccagg
     5341 gatcgttcag cccaaccgcc acctcaatgg cgcaaatccc cgccacggtg cctgtctcgc
     5401 tatcttccag cacgaacaca tagccctgct cgcttttggg cagttcgcct tgccaggttt
     5461 tgattgccct ttcgatacgc gcggaaagcg tggcttcatt ggcgggaagc gacgtcaggc
     5521 cgccgcccgt tttgctggca agctgcatca gcgccgagac atctgagcgc tcaacgggac
     5581 ggatgaccat catgatgaac ctcggctaac aaagtgttcg caagcagctg caaagcgatc
     5641 cagtccggtc gtcacctctt cttcgctgac attgagcgca ggcgcaaaac gcaccacgtt
     5701 gccacccgca atcagtacca taacgcctgc tttcgccgcc tcctgagaga tctgtttcgc
     5761 ttgtccggcg taatcggcat tcagtacaca gccaatcaac aaacctaagc cgcgaacttc
     5821 actgaacaaa ccgtagcggt gattaatggt attaagacgc tcaacaaacc agtcgtgacg
     5881 ctgtttaacg ccattaagca tctctggtgt gttgatgagc tccagcactt tgcccgccac
     5941 cgccgaggcc agcggattgc cgccataggt ggtgccatga gtgccaacgg tcatcacgct
     6001 ggcgcactct tcggttgtta acaacgcacc gaccgggaaa ccgccgccca gcgctttggc
     6061 ggtagttaac agatcaggtg tcacgccgta gtgcatatag gcatacagtt ccccggtgcg
     6121 cccgacgccg gtttgtactt catcaaaaat caacagcgca ttgtggcggt cacacaattc
     6181 acgcagacct tgtaaaaacg cgttgctggc tggcaccaca ccgccttccc cctggatggg
     6241 ttcgacaatc accgcacagg tggcgtcgtc aatcagcgcg ctggcagagt taatatcgtt
     6301 atatgcagca tgacggatat ctggtggcag tggcgcaaaa tcctgtgaat aggctggctg
     6361 cccacccgca ctgacagtaa acagcgtgcg accatgaaac gcatttttaa acgccacgat
     6421 accgctctta tggctgccgt agcggtcgtg agcgaattta cgcgccagtt ttagcgccgc
     6481 ttcgttggct tccgcaccgg agttacaaaa gaagacgcga tcggcaaacg tggcgtcgat
     6541 caattttttc gccagtcgca gtaccggctc gttggtgtaa ccgttgccgg tatgccagaa
     6601 cttactcgcc tgttcgttca gcgcttcacg cagttccgga tgcgcatggc ccagcgcgtt
     6661 caccgcaatg ccacccgcga agtcgatata ctctttcccc tgctgatccc acaagcgcga
     6721 accttcgcca cgtaccggta taaagggtgc cggagcgtaa acaggtatca tccattcatc
     6781 aaagttttca cgcgtaattg gctgagacat agcgacctct acagtaaata attcgctatc
     6841 tatatgttaa caataagtaa tgtttgcgct gtaaatgtag attgcagggt tcgtgccagc
     6901 cagtgataaa agtgcataaa cggcggaggc taactggaaa tcaaggagtt ataaccaaac
     6961 catatgcatt taaagtgcat ataaagtgaa tacgtttgcg atgtgggtga ataaaaagaa
     7021 taaaaaacgc aatgttatgc agaagtaaaa tataattctg gaattgtgat cattgacgaa
     7081 atttactgga aattactgcg ccattctgac gcagcgcgca ccaaaagcgg gcattttttg
     7141 cgccatcgtt gacatcatta acaaccatcg atcaaatcac ttaacaacag gcggtaagca
     7201 acgcgaaatt ctgctaccat ccacgcactc tttatctgaa taaatggcag cgactatgaa
     7261 atttgtctct tttaatatca acggcctgcg cgccagacct caccagcttg aagccatcgt
     7321 cgaaaagcac caaccggatg tgattggcct gcaggagaca aaagttcatg acgatatgtt
     7381 tccgctcgaa gaggtggcga agctcggcta caacgtgttt tatcacgggc agaaaggcca
     7441 ttatggcgtg gcgctgctga ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg
     7501 tgacgacgaa gaggcgcagc ggcggattat tatggcggaa atcccctcac tgctgggtaa
     7561 tgtcaccgtg atcaacggtt acttcccgca gggtgaaagc cgcgaccatc cgataaaatt
     7621 cccggcaaaa gcgcagtttt atcagaatct gcaaaactac ctggaaaccg aactcaaacg
     7681 tgataatccg gtactgatta tgggcgatat gaatatcagc cctacagatc tggatatcgg
     7741 cattggcgaa gaaaaccgta agcgctggct gcgtaccggt aaatgctctt tcctgccgga
     7801 agagcgcgaa tggatggaca ggctgatgag ctgggggttg gtcgatacct tccgccatgc
     7861 taatccgcaa acagcagatc gtttctcatg gtttgattac cgctcaaaag gttttgacga
     7921 taaccgtggt ctgcgcatcg acctgctgct cgccagccaa ccgctggcag aatgttgcgt
     7981 agaaaccggc atcgactatg aaattcgcag catggaaaaa ccgtccgatc acgcccccgt
     8041 ctgggcgacc ttccgccgct aatttagcag ctctcctggc tcaaactggg tcaggagaat
     8101 taaccttgag aaaaatcaac aaactgtcag taatgatttg ttgcctgccg tcctttgtta
     8161 taccgtcact gcgtttttag ttgtctgacc acttctctat tatcaagttt gatataggaa
     8221 actccacgat gaatgctgag cgtaaatttc ttttcgcctg tcttattttt gcgctggtca
     8281 tttacgctat ccacgctttc ggtttattcg atctgctcac cgatttaccc cacttacaga
     8341 cactcattcg ccagagcgga ttgttcggtt atagcctcta tattctgtta ttcatcattg
     8401 ccaccctctt tgtgttacca ggaagcatac tggtgatcgc tggaggaata gtttttggcc
     8461 cgctcttagg gacactactc tcattaattg ccgccacgct ggcctcctcg tgctcattcc
     8521 tgctggcgcg gtggctggga cgtgatttac tgctgaaata cgtgggccat agccatacct
     8581 tccaggccat tgaaaaaggc attgcgcgta acggtattga ttttcttatt ctgacccgct
     8641 taatcccgtt gtttccttac aatattcaaa attacgctta cggattaacc accatcgcct
     8701 tctggcctta tacccttatt tcggcactca cgaccctgcc cggtattgtt atttataccg
     8761 tgatggcaag cgatctcgcc aatgaaggca ttacgctgcg ctttatttta caactctgtc
     8821 tggcgggcct ggcgctgttt attctcgtcc agctcgcaaa actctacgcc cgacacaaac
     8881 atgtggatct gtctgcttcg cgccgcagcc cgcttactca cccaaaaaat gaaggataga
     8941 acgatgtcgc aacattattc agtgtcatgg aaaaaaggac tggctgcact ctgcttactg
     9001 gctgttgccg ggcttagcgg ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac
     9061 gacggacttt cgaacagcca accgctgcgc gtcgatgcca ataaccatac ggtaaccatg
     9121 ttggtgcaaa ttaatggtcg tttcctcacc gacgacactc gtcacggtat tgtgtttaaa
     9181 gatggctcca acggacataa atcgctgttt atggcttatg cgaccccgaa agcattttat
     9241 gaagccctga aagaggcagg tggtacgccg ggcgaaaaca tgacgatgga taataaagaa
     9301 acgactcatg tcacaggcag caaactggat atttcggtca actggcaagg ggcggcaaaa
     9361 gcgtattcct tcgatgaagt gattgttgac agtaatggca agaaactgga catgcgcttt
     9421 ggcggtaatt taacggcagc agaagagaag aaaacgggtt gcctggtgtg tctggatagc
     9481 tgcccggtcg gcatcgtcag caatgcaaca tacacttatg gtgcggtgga aaaacgcggc
     9541 gaagtgaaat tcaaaggcaa tgcctcagtt ctcccggcgg ataacacgct ggcaacggtt
     9601 acctttaaaa tcaccgaata aagccaggat aaaggatgat gaagatgcaa tcgcgtaaaa
     9661 tctggtacta ccgtataacc ctcatcatcc tgttgttcgc tatgctgctc gcatgggcgc
     9721 tgcttcccgg cgtccatgag tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc
     9781 aacagggtat agaacgcttt attcagtctt acggcgcact ggcggcagtt gtctcgttct
     9841 tgttgatgat tttacaggcc attgctgcac cgctacctgc gtttttgatc acctttgcca
     9901 atgcgtcgct gtttggcgcg ttctggggcg gcttgctgtc gtggaccagt tcgatggccg
     9961 gcgcggcgct gtgctttttt atcgccagag tgatgggccg cgaagtggtg gaaaaattaa
    10021 ccggcaaaac cgtgcttgac agtatggacg gctttttcac tcgctacggc aaacacacca
    10081 tcctggtctg tcggttattg ccttttgtcc ctttcgatcc aatcagctat gctgccgatc
    10141 tgacttcaat acgttttcgc tcgtttttta tcgccaccgg gcttggtcaa ttaccgacga
    10201 ctattgttta ttcctgggcg ggcagcatgt taacaggcgg tactttctgg tttgtcaccg
    10261 gactgtttat tctgtttgcc ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg
    10321 agcgccagaa gaggaatgac tgatgggttt accgccgctt agcaaaattc ctttcatttt
    10381 acgtccgcag gcgtggctgc atcgtcgcca ttacggcgag gtgctaagcc cgattcgctg
    10441 gtgggggcgg atcccgttta tcttttattt ggtgtcaatg tttgttgggt ggctggagcg
    10501 caaacgctca ccgctcgatc cggtagtgcg atcgcttgtc agcgcgcgca ttgcgcaaat
    10561 gtgcctgtgt gagttttgtg tggatatcac cagtatgaaa gtcgccgagc gtaccggcag
    10621 cagcgataaa ctgctggcag tggctgactg gcggcaaagc ccgctcttta gcgatgaaga
    10681 acggctggcg ctggagtacg ccgaagccgc aagcgtcaca ccgccaacag tcgatgatgc
    10741 cctgcgtacc cgactggctg cgcattttga cgctcaggcg ctcaccgaac tgacggcatt
    10801 gatcggcctg caaaacctgt cggctcgttt taattctgcc atggacattc ccgctcaggg
    10861 gctgtgccgt attccggaaa aacgttctta aggagagaag atgcgccatt gtgggtggtt
    10921 gctgggattg ttatcgctgt tttctttggc aacacatgcc agtgactggc aggaaattaa
    10981 aaatgaggcc aaagggcaaa ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa
    11041 ccgctatctc gactgggtta gcggcgagat gaaaacccat tacgctataa acctgaagat
    11101 tgttcgtctc gcggatgccg cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg
    11161 tcgtaaaacg ggcggctcgg tggatctgct ctgggtgaac ggcgaaaact tccgcacctt
    11221 aaaagaggcc aatttattac aaacgggctg ggcggagact ctgcccaact ggcgctatgt
    11281 cgacacacag ctgccggtgc gggaagattt ttcagtgccg acacaaggtg cggaatcgcc
    11341 ctggggcggc gcacaactga cgtttatcgc ccgccgcgat gttacgccac agccaccaca
    11401 aacgccgcaa gccttactgg agtttgctaa agccaatccc ggcacggtta cttatccgcg
    11461 cccaccggac tttaccggca cggcgtttct tgaacagttg ctgattatgc tgacgccaga
    11521 tcccgccgca ttaaaagaag cgccggacga tgcgactttc gcccgtgtca ctgcgccctt
    11581 gtggcaatat cttgatgcac tgcatccgta tttgtggcgc gaaggaaagg atttcccgcc
    11641 ttcgcccgcg cggatggatg ctctgctgaa agccggcaca ttgcgcctgt cgctgacctt
    11701 taaccccgcg catgcgcagc aaaaaatcgc cagcggcgat ttgccggcaa gcagttacag
    11761 ttttggcttt cgcgagggga tgattggcaa cgtgcatttc gtcaccattc cagccaacgc
    11821 gaatgccagt gctgcggcga aggtagttgc caatttcctg ctctcacccg atgcgcaact
    11881 gcgtaaagca gatcccgttg tctggggcga tccttctgtt ctcgatccgc aaaaactgcc
    11941 tgacgggcag cgcgaaatat tgcaatcaag aatgccgcag gatctgccgc cggtactggc
    12001 tgaaccgcac gcaggatggg tgaatgcact ggaacaagaa tggttacgcc gttacggtac
    12061 gcattaatct ttttgctgtg ggcgatggtg gcggtgattt atgcaccgct gatcccggca
    12121 gctctcacgc tgatatcgcc tgcgttgtcg ttgacacact ggcaggcgtt atttgccgat
    12181 ccacagttac cgcatgcatt actggcaacg ctggtatcga caaccatcgc ggcggtcggg
    12241 gcattgttga ttgccctgct ggtgattgtg gcgctatggc ctgggccgaa atggcagcgt
    12301 atgtgcgccc gtctgccgtg gctgctcgcc attccccatg tggcttttgc caccagcgcc
    12361 ctgctgctct ttgctgacgg agggctgcta tatgactatt tcccgtattt cactccgcca
    12421 atggaccgat ttggcatcgg tctgggcctc acccttgcag tgaaagaaag cgcatttctg
    12481 ctgtggatct tagcggcagt attgagcgaa aaacggctgt tgcagcaggt cattgtgctg
    12541 gattcgctgg gctacagccg ctggcaatgc ctgaactggc tgctgttgcc ctccgtcgcg
    12601 ccttcgctgg caatggcgat gctggcgatt gttgcctggt cgctttcggt cgtggatgtg
    12661 gcgattattc ttgggccagg taatccaccg acgctggcag taattagctg gcagtggtta
    12721 actcagggcg acgcggatca acaaacgaaa ggcgcactcg ccagtctgct gctgatgctg
    12781 ttactcgccg cctacgtttt gctgagctat ctgctatggc gcagctggcg gcgcactatt
    12841 ccccgcgtag atggcattcg caatgcctgc cacaccttta ttaccaggca ctacgctggc
    12901 gagtttttta cccttaaccg gtgtgctgtg tgtggttctg ctggcgatcc tcgcggatca
    12961 gtcgacgatc aatagtgaag cactcatcaa cagtctgaca atggggctgg tagcaacatt
    13021 catcgctttg ctcctgttac tgctgtggct ggaatggggg ccacagcgtc gccagttgtg
    13081 gctatggttg cccattctat tgcccgatct gccactggtg gcgggccagt acacgctggc
    13141 gctatggctg aacctagatg gaagctggac ggcggtggtc tgggggcatc tgctgtgggt
    13201 aatgccgtgg atgctgttta tcctgcaacc cgcctggcag cgcattgatt cacggttaat
    13261 tttgattgcg caaacactgg gctggtcgcg ggccaaaatc ttcttttacg tgaaatgccc
    13321 actcatgttg cgccctgtgc tgattgcctt cgcggtggga ttttcagtca gtattgcgca
    13381 gtatatgccg acgctgtggc tgggcgcggg gcgttttccg acgctcacca ctgaggcggt
    13441 ggcattaagc agcggcggca gcaacggtat tctcgccgcc caggctttat ggcaactgtt
    13501 gttaccgctt attatttttg ccctgaccgc gttagtcgca aaatgggtag gttatgtcag
    13561 acaaggactc cgctaatgct ctgcgtgaaa aatgtttcgc tacgtttacc agaaagccgc
    13621 ttgctgacaa acgttaactt tacggttgat aaaggtgaca tcgtcacgtt aatggggccg
    13681 tctggctgtg gaaaatccac tctgttttca tggatgattg gtgcactggc tggacagttt
    13741 tcttgtacag gtgagctatg gctcaatgag caacggattg acatgctacc caccgcacag
    13801 cgtcagattg gcattctttt ccaggatgca ctgttatttg accagttcag cgtcgggcaa
    13861 aatttactgc tggcgctact gtcgacgctc aaggggactg ccaggcgcaa tgccgtgaaa
    13921 gacgcgcttg atcgagcggg actggctgaa acttatcacc aggaccctgc gacattatca
    13981 ggcggtcagc gagcgcgggt ggccctgctg cgcgcccttc tcgctcagcc aaaagcgtta
    14041 ctcctggatg agccattcag ccgtcttgat gtggctctgc gcgataattt tcgccagtgg
    14101 gtgttcagcg aagttcgcga actggcgatc cccgtcgttc aggtaacgca cgatctccag
    14161 gatgttcctg ctgatagttc tgttctggat atggcgcagt ggtcagaaaa ttacaacaaa
    14221 ctgcgataac gcaaagtttt tctcaatgcg tcagttcaga atggcgcact caaaactaca
    14281 atgtcgggat tttcgatgaa acgtgtttct caaatgaccg cgctggcaat ggctttaggg
    14341 ctggcttgcg cttcttcgtg ggccgctgaa ctggcgaagc ctcttacact tgaccagctt
    14401 caacaacaaa atggcaaagc gatagatact cgccccagcg cgttttataa cggctggcca
    14461 caaaccttaa atggcccttc tggtcatgaa cctgccgcct taaacctctc tgccagctgg
    14521 ctcgacaaaa tgagcaccga acagctcaac gcgtggatca agcaacataa cctgaaagcc
    14581 gatgccccgg tggcgctgta cggtaatgac aaagatgtcg acgccgtcaa aacgcgactg
    14641 caaaaagcag gttttacgca tatctccatt ctgagtgacg cgctaagcga accttcccgt
    14701 ctacaaaaac tgccgcactt tgagcagctg gtttatccgc aatggctgca cgacctgcaa
    14761 caaggtaaag aggttacggc gaaacctgcc ggtgactgga aagtcattga agcggcctgg
    14821 ggcgctccta agctttacct tatcagccat attcccggtg ctgactacat cgataccaac
    14881 gaagtggaaa gcgaaccgct gtggaacaaa gtttctgatg aacaactaaa agcgatgctg
    14941 gcaaaacacg gcattcgcca tgacaccacg gtcattctgt acgggcgtga cgtatacgct
    15001 ggagcgcgtg tggcgcaaat tatgctctac gcaggcgtga aagatgtgcg tctgctggat
    15061 ggcggctggc aaacctggtc cgacgcggga ctgcctgttg agcgaggaac gccaccgaaa
    15121 gtgaaagcgg aaccggattt cggcgtgaag atcccggcac aaccgcagtt gatgctcgat
    15181 atggaacagg cccgtggact gctgcatcgc caggatgcat cgctggtgag cattcgttcg
    15241 tggccagaat ttatcggtac gaccagcggt tacagctata ttaaaccaaa aggtgaaata
    15301 gccggagcac gttggggaca cgctggtagc gactcgacgc atatggaaga ttttcataac
    15361 ccggatggca ccatgcgcag cgccgatgat attaccgcta tgtggaaagc gtggaatatc
    15421 aaaccagagc agcaagtttc attctactgc ggcaccggct ggcgcgcgtc cgaaaccttt
    15481 atgtacgcac gcgccatggg ttggaagaat gtttccgtgt atgacggcgg ctggtacgaa
    15541 tggagcagcg atccaaaaaa tccggtagca accggtgaac gcggcccgga cagtagcaaa
    15601 taacattgca attactgacg ctggagcgac ttcagcgtca gatacccact ccacacccgc
    15661 gtaaaggtcg tcatccagca caacgcaccg aagatccacg caaaccacgc aaaccatgca
    15721 gggaataaac atcccagcac aaacagtaag atcgtttctg tgccttcggt taatcccccc
    15781 agatagtaaa acgacttatg tgcataaccg gggttatcaa tctgatgttt tgctgccagt
    15841 gcagcaaagg cgagaaaact gctgcccgtg ccgataaacg caaacaacaa ccggccgcct
    15901 gccagcgcat tttgctccgg tgcagcaaga ataaagccaa acggcaccag cgcgtaaaag
    15961 a
//
LOCUS       DAESHI010000096        15881 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.097, whole genome shotgun sequence.
ACCESSION   DAESHI010000096 DAESHI010000000
VERSION     DAESHI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15881
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.097"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            1..1453
                     /locus_tag="KW011_003133"
                     /pseudo
     CDS             1..1453
                     /locus_tag="KW011_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-mannosidase"
     gene            complement(1541..2512)
                     /gene="lpxM"
                     /locus_tag="KW011_003134"
                     /gene_synonym="msbB"
     CDS             complement(1541..2512)
                     /gene="lpxM"
                     /locus_tag="KW011_003134"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBH7661407.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(2632..3954)
                     /gene="mepM"
                     /locus_tag="KW011_003135"
     CDS             complement(2632..3954)
                     /gene="mepM"
                     /locus_tag="KW011_003135"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HBH7661408.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(3970..4902)
                     /gene="znuA"
                     /locus_tag="KW011_003136"
     CDS             complement(3970..4902)
                     /gene="znuA"
                     /locus_tag="KW011_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HBH7661409.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4981..5736
                     /gene="znuC"
                     /locus_tag="KW011_003137"
     CDS             4981..5736
                     /gene="znuC"
                     /locus_tag="KW011_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HBH7661410.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            5733..6518
                     /gene="znuB"
                     /locus_tag="KW011_003138"
     CDS             5733..6518
                     /gene="znuB"
                     /locus_tag="KW011_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HBH7661411.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(6665..7675)
                     /gene="ruvB"
                     /locus_tag="KW011_003139"
     CDS             complement(6665..7675)
                     /gene="ruvB"
                     /locus_tag="KW011_003139"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBH7661412.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(7684..8295)
                     /gene="ruvA"
                     /locus_tag="KW011_003140"
     CDS             complement(7684..8295)
                     /gene="ruvA"
                     /locus_tag="KW011_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBH7661413.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            complement(8434..8499)
                     /gene="yobI"
                     /locus_tag="KW011_003141"
     CDS             complement(8434..8499)
                     /gene="yobI"
                     /locus_tag="KW011_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HBH7661414.1"
                     /translation="MYIFITHLFTEYVILKYLLPI"
     gene            8572..9174
                     /locus_tag="KW011_003142"
     CDS             8572..9174
                     /locus_tag="KW011_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HBH7661415.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(9176..9697)
                     /gene="ruvC"
                     /locus_tag="KW011_003143"
     CDS             complement(9176..9697)
                     /gene="ruvC"
                     /locus_tag="KW011_003143"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBH7661416.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(9732..10472)
                     /locus_tag="KW011_003144"
     CDS             complement(9732..10472)
                     /locus_tag="KW011_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBH7661417.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(10501..10953)
                     /gene="nudB"
                     /locus_tag="KW011_003145"
     CDS             complement(10501..10953)
                     /gene="nudB"
                     /locus_tag="KW011_003145"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HBH7661418.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(11071..12843)
                     /gene="aspS"
                     /locus_tag="KW011_003146"
     CDS             complement(11071..12843)
                     /gene="aspS"
                     /locus_tag="KW011_003146"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBH7661419.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            13153..13719
                     /locus_tag="KW011_003147"
     CDS             13153..13719
                     /locus_tag="KW011_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBH7661420.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            14074..14322
                     /locus_tag="KW011_003148"
     CDS             14074..14322
                     /locus_tag="KW011_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBH7661421.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            14923..>15881
                     /locus_tag="KW011_003149"
     CDS             14923..>15881
                     /locus_tag="KW011_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBH7661422.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGPQI
                     YFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEE"
BASE COUNT         4036 a         4030 c         3643 g         4172 t
ORIGIN      
        1 attttttata actattacta ataatatgac atcaattctt cgcagtgagt ttgatgaaat
       61 atggaaagcg atgctgacct tgcaattcca tgacatcctt cccggctcat gtatttcaag
      121 agtttatcat gaaactgaaa aggaatatct taaactagaa gcaaaaaccg agaaaattat
      181 ttctgatgcg cagtctactt tattaagtaa aattgacact tcttcttata aagatccgca
      241 cattttgttt aataccacct gttttgctcg taacgaatgg atcaatataa acaacaactg
      301 gctcaaagca cgagtaaact cttatggtta tgctgtgatt gttccaaaga ataagatcgt
      361 aaatggtctg aaggcagaat ctcaaagtat tgaaaataat tacattaaac ttcttttttc
      421 agaaagcggt gatttaattt cgctatatga taaaagatat ggtaaagaat acattactga
      481 aaatatgcac agtgagatcc gtgcatacca cgaagatgca ggttttttcg cggcctggga
      541 ttttgcttca aattatcgtg atggggaatc atatgtttta ctcgctgaaa aaatgacaac
      601 tgtgatcagc gggcctaaaa ctacgatgac gctgatttat cactacaatt cctcttatct
      661 tcgtttagcc tttaccctga cacaggatag tccacgcgtt gatgtccaaa cgtttattga
      721 ttggcacgaa cctaatgttt ctctcaaagt aaaattcccg gtgtcagtac aaacatctct
      781 tgcacaatgt caaattcagt tcggtgtaat tgacagacca acgcatagcg atgattcgtt
      841 cgcttttgcc aaagatgaaa taccagcaca tcactgggtc gacctttctg atcgagaaac
      901 tggcatagct ttaatagcaa aaaacaaata tggctatcgt gtcaaagacc agacgcttga
      961 attaactttg ctgcgttcgc agcataaacc tggggaagtt gttaagacag acaattatga
     1021 taatttcctg attaataatt ttgcagatat tggtaaacaa aaattcatat tctccttatt
     1081 tcctcatcat ggagattacc gtgtgggtgg tgttattaat caagcctata taatgaatca
     1141 tcccttacag gtcgtccctg tcaaaccgca cccgggtgaa ttaccccctt cactatcatt
     1201 ttttgctatc gatactaaca gcgttatcat cgaaacaata aaattagctg aagacggcga
     1261 cggtataatc gtaagattat atgagagtca tggattagaa atttcagcca tgctttcgtg
     1321 agtatgtaat tatcactacg ttcaatatgt cgatcttcaa gaaaataagt tagatgacag
     1381 tttttattgc aatcaatatt gcaatcttga gttcaaacct tttgaaatta tcacgctacg
     1441 attaactcaa tagcgtacta acctaaaaac tcaaaatagt gggaacactc attgagattt
     1501 tttatcgagt acagcctctc aataatttga gaggcttttt ttatttgata ggataaagat
     1561 ctttacgctt atacggttgg atttcgcccg gtttgcgagt tttcagcaat tttaatatcc
     1621 aggtgtattg ttctggtcgc ggaccaacaa aaatctcgac ttcttcattc atccgccgcg
     1681 caatcgtatg atcatccgcc tctaacagat catccatcgg tgggcgcacc tgaatcgtca
     1741 gacggtgcgt cttgccatca taaatcggaa acagcggaac aacgcgcgca cggcacactt
     1801 tcatcaaacg accaatcgcg ggcaacgtcg ctttataggt ggcaaagaaa tccacaaatt
     1861 cgctgtgctc tgggccatga tcctgatcgg gtaaataata tccccagtac ccctgacgta
     1921 ccgactggat gaatggttta ataccgtcat ttctcgcatg cagacgaccg ccaaagcgac
     1981 gacgcaccgt gttccagaca taatcaaaaa ccgggttgcc ctgattatgg aacatcgctg
     2041 ccattttctg cccttgcgag gccatcagca tggcaggaat atcgacggcc caaccgtgcg
     2101 gcaccagaaa gataactttc tcgttattac gcctcatctc ttcgatgatc tccagccctt
     2161 gccagtcaac gcgcggctga attttctccg gtccgcgtat tgccaactca gccatcattg
     2221 ccatcgcttg cggcgcggtg gcaaacatct catcaacaat cgcttcgcgt tcagcttcac
     2281 tacgttctgg aaagcagagc gacagattga ttaacgcacg acggcgtgag ctttttccca
     2341 gtcgtccggc aaaacgtccc agccgtgcca gaatgggatc acggaacttt ggcggcgtta
     2401 aagcgatacc cgccatcgct gctacaccca gccatgctcc ccagtagcgc gggtggcgaa
     2461 aggatttatc aaactcagga atgtattcgc tattattttt tttcgtttcc atgcttttcc
     2521 agtttcggat aaggcaaaaa tcaatctggt gatagtgtag cggcgcaact tgccccgcac
     2581 caaataaaaa aagccgatac tgactgcgta ccggctgcga atggatgtta attaatcaaa
     2641 ccgtagctgc ggcacaatct ctttggcctg cgccaggaat tcgcgacgat cggagccggt
     2701 cagcccttcg gtacgcggca gttttgccgt cagcgggttt acggcctgct ggtttatcca
     2761 tacttcatag tgcagatgcg gcccggttga acgtccggta ttaccggaaa gcgcgatacg
     2821 gtcgccacgt ttcaccttct gtcccggttt caccagaatc ttgcgcaagt gcatataacg
     2881 cgtggtgtag ctgcgaccat gacgaatagc cacataataa cctgctgcgc cactgcgttt
     2941 ggcaaccacc acttcaccgt cacccactga aagcactggc gtaccctgcg gcatggcgaa
     3001 atcaacacct ctgtgtgctg caacgcgacc ggtcaccgga ttagtacgac gcgggttaaa
     3061 gttagacgag atacggaact gtttcgccgt cgggaatcgc aagaatcctt tcgccagacc
     3121 agtaccgtta cgatcgtaga atttgccatc ttcagcgcgg atagcgtaat aatctttacc
     3181 ttctgaacgc aaacgtacgc ccagcagctg gctttgctca cgtttaccat caagcatttc
     3241 tcgagacatt aacaccgcaa attcatcgcc ttttttcagt ttgcggaaat ccatttgcca
     3301 ctgcatggct ttaatcactg cgctcacttc ggcgctggtt aaaccggcgt ttctggcgct
     3361 ggcaacaaag ctccccccga cggtaccttt cagcagattg ttaacccact ctccttgctg
     3421 catttcgctg gtcattttaa aaccgttagc ggcagtacgg tcataggttc gggtttcacg
     3481 acgagacact tcccaggtga ggcgctgcag ttcgccgtcc gcggttaatg tccaggagag
     3541 ttgttgaccg attttcaggt tacgcaattc tttgtcggcc gcagccagtt gggtgatatc
     3601 acccatatca ataccatact gattgagaat gctgcttagc gtatcgccag tggaaacaac
     3661 atattcatgc acgcccgctt caccggcgat tttgtcatcc agttcgtcct ggggaatggc
     3721 ttcatcttct tgtgcagctt gatcaatcgg ctcactggct tcaggtaaga gcgaacgaat
     3781 ttcgttctgt tccagctcaa tggttttgac aattggcgtg gcatcgcggt gataaacata
     3841 gggccgccag acagcgacgg ccagagtaag aacggtgagc gaccccaaca taacgcggtg
     3901 tggtcgcggt aaattattaa acgccagggc gacagagcgg gctatctgtt gcacgtaatc
     3961 acttcctcat taatctcctt tcaggcagct cgcatactgg ttggctaatt gactcaggaa
     4021 ttctgaatag cttgttttac ccagtttgat attcgtcccc aggggatcca acgttcccat
     4081 acgaacggat gtccctcgtg cgacgctctc aacgaccgct ggcctgaact gtggctcagc
     4141 aaaaacgcag gttgcttttt gctcaaccaa ctgtgttctt atttcatgta aacgctgcgc
     4201 gccaggttga atctcagggt taacggtaaa atgaccaagc ggtgtcagtc cgaactgttt
     4261 ttcgaaatag ccgtaagcat cgtgaaaaac gaaataacct ttccccttga gcggcgcgag
     4321 ctcgttacca acctgcgttt cggttgaggc taattgtgcc tcaaaatcct tcaggttggc
     4381 gtcaagtttg gctcgacttt gcggcataag ttccactaat tttccatgga ttgcaaccgc
     4441 tgtagcccgc gctatctctg gggaaagcca aagatgcatg ttgaaatcgc cgtgatggtg
     4501 atcttcgtca cttttttccg cgtggtcgtg atcatcatca tcgccgtgaa tacttttcat
     4561 cagtagcggt ttcacatctt caagctgcgc aatcgttacc tgcttcgctc ctggtaattt
     4621 gcttaccggt ttttgcataa acgcttccat ctccgggcca acccaaacga ctaagtccgc
     4681 gttctgtaag cgttttacat ccgatgggcg cagtgaataa tcatgttctg aagcgccgtc
     4741 aggaagtaaa acctctgttt ctgttacccc atcagcaatg gcagaagcga tgaacccaac
     4801 gggtttaagc gaagcgacaa cggcggcatc tgcggcctgt gttgcacctc cccagagagc
     4861 ggcggataat gctgcgaaaa gaagcgtttt tttatgtaac ataatgcgac caatcatcgt
     4921 aatgaatatg agaagtgtga tattataaca tttcatgact actgcaagac taaaattaac
     4981 atgacaagtc tggtttcctt ggaaaatgtc tcggtttctt ttggccaacg ccgcgtcctc
     5041 tctgatgtgt cgctggaact taaacctgga aaaattttga ctttacttgg gccaaatggc
     5101 gcaggtaagt cgacactggt acgggtagtg ctcgggctgg taacacccga tgaaggggtt
     5161 atcaagcgca acggaaaact gcgcatcggc tatgtaccgc agaagctgta tctcgacacc
     5221 acgttgccac tgaccgtaaa ccgtttttta cgcttacgcc ctggtacaca taaagaagat
     5281 attttgcctg cactgaaacg tgtccaggcc gggcacctga ttaacgcacc gatgcaaaag
     5341 ctctctggtg gcgaaacgca gcgtgtactg ttagcgcgag cattgttaaa tcgcccgcaa
     5401 ttattagtgc tggatgaacc cactcaaggc gtggatgtaa atggccaggt ggcgttatat
     5461 gaccttattg accaactgcg tcgtgaactg gattgtggcg ttttaatggt ttctcacgat
     5521 ctgcatctgg tgatggcaaa aaccgatgaa gtgctgtgcc tgaatcacca catttgttgt
     5581 tccggcacac cggaagttgt ttccctgcat ccggagttta tttcaatgtt tggtcctcgt
     5641 ggtgctgaac aactgggtat ctatcgccat catcataacc atcgtcacga tttacaggga
     5701 cgaattgttt tgcgtcgggg aaatgatcgc tcatgattga attattattt cccggttggt
     5761 tagccgggat catgctcgcc tgtgccgcgg gtccgctggg ttcgtttgta gtctggcgtc
     5821 gtatgtctta tttcggtgat acgctggctc atgcctcatt acttggtgtc gcgtttggtt
     5881 tgttgctgga cgtgaatcca ttctatgcgg tgattgccgt tacgctgctg ctggcgggcg
     5941 gtctggtatg gctggagaag cgtccacagc tggcgatcga cacgttatta gggattatgg
     6001 cgcacagtgc cctgtcgctg ggcctggtgg tcgttagtct gatgtctaat attcgtgttg
     6061 atttgatggc ttacctgttc ggtgatttgc tggcagtgac gccagaagat ctcatctcta
     6121 ttgcgattgg cgtggtcatc gtggtggcta ttttgttctg gcaatggcgc aatttgctgt
     6181 cgatgacgat tagcccggat ctggcgtttg ttgatggtgt gaaattacag cgcgtgaaat
     6241 tgttgttgat gctggtgacg gcattgacga ttggtgtagc gatgaaattt gtcggtgcgt
     6301 tgattattac ttcgttgctg attattcctg cagctactgc gcgtcgcttt gcccgtacgc
     6361 cggaacagat ggctggtgtc gctgttttgg tggggatggt ggcagtaact ggcggtttaa
     6421 ccttttccgc gttttacgat acgccggcgg gtccgtcggt agtgctatgt gctgcactgt
     6481 tatttattat cagtatgatg aaaaagcagg ccagctaatc tgtcgctgaa cacatttgtc
     6541 ggatgcggcg cgagcgcctt atccgaccta cggttcgcta tctctggtag gccggataag
     6601 acgcgaatag cgtcgcatca ggcacactgc caatgtcgga tgcggtgcga gcgaccaatc
     6661 cgacttacgg catttctggc ggcgttatgc caaagtgatt ccacgcccgc gtcgtcgcca
     6721 tacgcccacg cggtgtacgc tgcaaaaagc cttgctgaat cagataaggc tccagcacat
     6781 cctcaatggt ttcacgttct tcgccaatgg ctgccgccag gttatccaga cctaccggcc
     6841 caccaaagaa cttatcgatt accgccagca acaatttgcg gtccatataa tcgaaacctt
     6901 cagcatcgac attcaacata tccagcgcct gagcagcgat atctgccgag atggtgccat
     6961 cgtgcttcac ttcggcgaaa tcacgcactc gacgcagcag acggttggca atacgtggcg
     7021 taccgcgagc gcgacgagca acttccagcg cgccgtcatc actcatctca agccccataa
     7081 agcgtgcgct gcgactgacg atatattgca gatccggcac ctgataaaac tccagacgtt
     7141 gcacaatacc aaaacggtcg cgcaacggtg atgtcagcga acctgcgcgc gtggttgcac
     7201 caatcagggt aaacggcggc aaatcaattt taatggagcg tgccgccgga ccttcaccaa
     7261 tcatgatatc cagttggtag tcttccattg ccgggtacag cacttcttca acaactggcg
     7321 atagacggtg gatctcatca ataaacagca cgtcatgcgg ttcaaggtta gtgagcatcg
     7381 ctgccagatc gcccgccttt tccagcaccg gaccagaagt cgtgcgtaaa ttaacgccca
     7441 tttcattggc gacaatgttg gcaagcgtag ttttacccaa ccccggagga ccaaaaatca
     7501 acagatgatc gagggcatcg ccgcgcagtt tcgctgcttt gatgaaaatc tccatctgtg
     7561 aacgaacctg cggctgacca acatactctt ccagtaattt agggcgaatg gcgcgatctg
     7621 ccccatcttc cggcaaagtg gtaccggcag aaatcagacg gtctgcttca atcatccttt
     7681 acctcataac gcggcgcgta gggcttcgcg aattaatgtt tcactgctgg cgtcagtgcg
     7741 agcgattttg ctcaccatgc ggcttgcttc ttgtggttta tagcccagcg ccaccagcgc
     7801 ggcaaccgct tcctgttcag catcgtcggt tgccgggctg gcaggcgacg tgagcaccag
     7861 atcggcggct ggcgtaaaga gatcgccgtg caaaccttta aatcggtctt tcatttcgac
     7921 aattaaacgc tcggcggttt ttttaccaat gcccggcagt ttcaccagtg ctcccacttc
     7981 ttcacgttca acggcattaa cgaactgctg cgctgacatt ccggagagga tcgccagcgc
     8041 caacttcggg ccgacgccgt tggttttgat taactcttta aacaacgtgc gctcttgttt
     8101 attgttaaaa ccgtacagca gttgcgcgtc ttcacgcacc acaaagtggg tgaaaacgat
     8161 cgcttcctga cccgcttcag ggagttcata aaaacaggtc atcggcatat gcacttcata
     8221 gcctacgccg cccacttcaa ttaacaccag cgggggttgt ttttcaatga tgatgcctct
     8281 gagtctgcct atcacatgac gctcctgcgt aatgaatcaa agataatgct gtatgataaa
     8341 aaaatgctgg atagatatcc agcgaaggat gaagaaaact tgcgaggtgt ctcgatgaac
     8401 tgaaaaatgg cacagtataa tttattctac aggttatatt ggaagcaaat attttaatat
     8461 tacatattca gtgaataaat gcgtaataaa aatatacatt gcgcctcctg aaaaaaaata
     8521 aattttttat gctattacgt atattcgtat ctatttcaat ggaatgacaa cgtgaatatt
     8581 aattaccctg ctgaatatga aattggagat atcgtcttta catgtatagg tgctgcctta
     8641 tttggtcaaa tatcagctgc atcaaattgc tggagtaatc acgtcgggat cattatcggt
     8701 cataacggtg aagacttcct ggttgcagaa agccgggttc ccctttcaac catcaccacg
     8761 ctatctcgtt ttattaaacg ttcgtctaat caacgctatg ctataaagcg attagacgct
     8821 gggctgacag aacaacaaaa acaacgaatt gttgaacagg ttccctcccg gctacgcaaa
     8881 ctttaccaca ccggttttaa atacgaatct tcgcgccagt tctgttcaaa atttgttttt
     8941 gatatttata aagaagcgct atgcattccg gtgggtgaaa tagaaacgtt tggagaactg
     9001 ttaaatagca atccgaatgc aaaactcact ttctggaaat tctggttctt aggttctatt
     9061 ccgtgggagc gtaaaaccgt cacgccagcc agtttgtggc atcatccggg tctggtgttg
     9121 attcacgcgg agggagttga aacgcctcag cctgaactga ccgaggcggt ataacttaac
     9181 gcagtcgccc tctcgccagg ttcagccgcg attcgctcat ctgcattgca ttctggctga
     9241 cgtgacaatg ggtaatcgcg atagctaacg catcggcggc atccgcctgt ggattagcgg
     9301 gcagtttcag caaggtgcgg accatatgct gcacctggct tttttcggca ctaccaatac
     9361 ctaccaccgt ttgctttacc tgacgtgccg cgtattcaaa taccggcaat tcctgattca
     9421 ccgccgccac aatcgccacg ccgcgcgcct gccccagttt cagagccgag tcggcatttt
     9481 tggccataaa gacttgttca atggcgaaat aatctggctg gaactgggtg atgatttccg
     9541 tcacgcccgc atagatgagc ttcagacgag acggtaaatc atccactttg gtgcgtatgc
     9601 atccgctacc caggtaggac agttgcctgc ctacctggcg gatgacacca tagccgatca
     9661 cgcgcgaacc cgggtcaatg ccgagaataa tagccatcac gcgtctccgt tttgctgttt
     9721 agcaggcccc atcagagagt tgctgcaacc tcatcagaga tttcaccgtt atggtagact
     9781 tcctgcacgt cgtcgcaatc ttccagcata tcgatcagac gcatcagttt cggtgcggtt
     9841 tctgcatcca tatcagcttt ggtggacggg atcatggaaa cttccgcgct gtctgctttc
     9901 agacctgccg cttccagagc gtcgcgcact ttacccattt cttcccatgc agtgtagacg
     9961 tcaatcgcgc cgtcatcata ggtcacaacg tcttcagcac ctgcttccag tgctgcttcc
    10021 ataatggtgt cttcatcgcc tttctcgaag gagatcacgc cttttttgct gaacagatag
    10081 gcaacggaac catcagtacc gaggttaccg ccacatttgc taaatgcatg acgcacttca
    10141 gcaacggtac ggttgcggtt gtcagacaga cattcaatca tgattgccgt gccgccagga
    10201 ccgtaacctt cgtagatgat ggtttccatg tttgcatcat catcaccgcc cacaccacgt
    10261 gcaattgcgc ggttcagtgt gtcacgggtc atgttgttag acagtgcttt atccaccgcc
    10321 gcacgcagac gcgggttagc gtccggatcg ccaccgccca gcttagccgc ggttaccagc
    10381 tcacgaatga ttttagtgaa gattttaccg cgcttagcat cctgcgcagc tttacgatgt
    10441 ctggtgttgg cccatttact atgacctgcc ataaaaatat ctccagatag ccctgcctgt
    10501 tcaggcagcg ttaattacaa actgttcaat cgcctgccgg ttgctccagg acttagtgag
    10561 cgccgcggca gcaggcgcat caagccactt gtaagccaga tgttcagtga agactacctg
    10621 gcgttcgtgc gggagcgcaa gacagaacca tgattccgta ttacgcgtca cgcccggcgc
    10681 atagcgatga cgtaaatgtg aaaaaatttc aaactctacc gtgcgctgac agtcaattaa
    10741 ggtcagttgt tcagcgacaa catcaatggt gacctcttcc tttacttcgc gcatggcagc
    10801 ttgcggcgcg gtttcaccct cttccacgct gccggttacc gactgccaga aatcgggatc
    10861 gtcacgccgc tgcaacatca gcacccgttt cgtatcttgt gcatagatga ccactaagat
    10921 cgaaacggga cgcttataca ctttatcctt cacgctgcct cttcgttgac tgccttcgct
    10981 catcccattc acatagttat ctatgctcat gggagttcac tcagttgccg ccttgatgca
    11041 acgcgaatga tttcgtgtat ttgagtcata tcagttattc tcagccttct tcacaacctg
    11101 aatgctcagc tcagccagta cagtcgggtt agcaaagctc ggtgcttcag tcatcagaca
    11161 cgctgccgcc gtggttttcg ggaaggcgat aacgtcacgg atattgtcgg tgccagtcag
    11221 cagcatggtc agacggtcaa gaccgaatgc cagacctgcg tgcggcggag taccgtattt
    11281 cagggcgtcg agcaggaagc cgaatttctc gcgctgttcc tcttcgttga tacccagaat
    11341 accaaacacc gtctgctgca tatcaccatt atggatacgt actgaaccac cgcccacttc
    11401 gtaaccattg atgaccatat cgtaagcgtt cgccaccgca ttttccggtg cagctttcag
    11461 ttctgcagca gtcatgtctt tcggtgaggt gaacggatgg tgcattgccg tcaggccgcc
    11521 ttcaccgtcg tcttcaaaca tcgggaagtc gataacccac agcggtgccc atttgctttc
    11581 gtcggtcaga ccaaggtctt tacccacttt caggcgcagt gcgcccatcg cgtcggcaac
    11641 aattttcttg ttgtcggcac cgaagaaaat catatcgcca tcttgcgcgg cagtacgatc
    11701 caggatggct tcgatgattt ctgcattaag gaacttcgct accgggctgt tgataccttc
    11761 cagacctttc gcgcgttcgt taactttgat gtaagccaga cctttcgcgc cgtagatttt
    11821 aacgaagtta ccgtattcgt cgatctgctt acgggtcagc gatgcgccac ccggaacacg
    11881 tagagcggca acacggcctt tcggatcgtt cgccggcccc gcaaatactg caaactcaac
    11941 agatttcagc agatcggcaa cgtcggtcag ttccatcggg ttacgcagat ccggtttatc
    12001 agaaccataa cggcgttctg cttccgcaaa ggtcattacc gggaaatcgc ccagatccac
    12061 gcccttcact tccagccaca aatgacgcac cagcgcttcc atcacttcac gcacttgcgg
    12121 cgcggtcatg aaagaagttt ccacatcgat ctgagtaaat tcaggctgac gatcagcgcg
    12181 caggtcttcg tcacggaagc atttaacgat ctgatagtaa cggtcaaaac cggacatcat
    12241 cagcagctgt ttgaacaact gtggggattg cggcagtgcg tagaatttac ctttgtgcac
    12301 acgagaaggc accaagtagt cacgcgcgcc ttccggcgtg gctttggtca gcatcggagt
    12361 ttcgatgtcg aggaagccgt ggtcatccat aaaacggcgc accaggctgg tgattttagc
    12421 gcgggttttc aggcgctgcg ccatttccgg gcgacgcagg tcgaggtagc ggtatttcag
    12481 acgcgcttct tcggtgttga cgtggttaga gtcaagcggc agaacatctg cgcggttgat
    12541 gatagtcagc gaggacgcca gcacttcaat ttcgccggtc gccatatcgc ggttaatatt
    12601 tttttcgtca cgcgcacgta cggtgcccgt aacctgaatg cagaactcat tacgcagttc
    12661 agaggccagc tttaacgcgt ccgcacgatc cggatcgaaa aatacctgca cgataccttc
    12721 gcggtcgcgc atatcgatga agatcaggct accaagatca cgacgacggt tgacccaacc
    12781 acacagagtc acctgctgcc ccacgtggga caaacggagc tgtccacaat attctgtacg
    12841 catgagatat cccttaactt agctgccggc ggatgccccc tgctgcgcag gtgaccaagt
    12901 cgcagcgtta gctgtatgtc acaactgaat gaaaaaaggc ggctattata ctggaaattc
    12961 tgccgcaccg taagagcctg gcccgcgctg taacgcctcg ttaccacttt atatcgggcc
    13021 tgaaatcaga ctctacgcca gtttgctata aaggtgttgc ccgaactcat aaaaattaac
    13081 aaaatttgtc gttccgccat cggctaatcg cattaaggtg tgaggcacga ttttgttttt
    13141 tcaggagtca gcatgcttga acttaatgct aaaaccactg cgttggtggt gatcgattta
    13201 caagaaggca tcttaccttt tgccggtggt ccacatactg ccgatgaggt agttaatcgc
    13261 gccgggaagc tggcggcgaa atttcgcgcc agcggtcagc ccgtgtttct ggtgcgagtt
    13321 ggctggtctg ccgattacgc cgaagcatta aaacagccgg ttgatgcccc ctcccccgct
    13381 aaagtgttgc ccgaaaactg gtggcaacat ccagctgcat taggtgcaac tgacagtgat
    13441 atcgaaatca tcaaacgtca gtggggtgcg ttttacggta cggatctgga gttgcaactg
    13501 cgccgccgag gtatcgatac aatagtgtta tgtgggatct cgaccaatat cggtgttgaa
    13561 tccaccgccc gcaatgcctg ggaacttggc tttaatctgg tgattgccga agacgcctgt
    13621 agtgccgcca gcgccgagca acacaataac agcattaatc atatctaccc gcgcatcgcc
    13681 cgtgtgcgta gcgtggaaga gatccttcac gcgttatgat ttacatcggt ctaccgcaat
    13741 ggtcgcatcc taaatgggtg cggttgggga tcaccagcct tgaagagtat gcccgccact
    13801 ttaactgcgt gatacgggat ttttaaaaat cactaaagaa cgcccaagag catgtgtttt
    13861 ctttagaata atcaaagcgt tggattgcca tgccatatga aaatcggcaa agttcatatg
    13921 taacaaagtg tcccaatcat gccccaaaat gccccacaac aaatattttt gccccatgca
    13981 tgccccaaaa aatcactttc cctcactctt gacactgttt atccatacag ttaaaaataa
    14041 tactgtataa aaacacagtg tagagggatt tttatgcgta ttgaaatctg catagccaaa
    14101 gagaaaatga ctaaaatgcc aaccggtgct gtggatgcgt taaaggaaga attaacccga
    14161 cgcatcagta aacgttatga cgatgtagag gtgatcgtaa aagccaccag caacgatggc
    14221 ctttctgtta cgcgcaccgc cgataaagat tcagctaaaa cttttgttca ggaaactctg
    14281 aaagatacct gggagtctgc tgacgagtgg tttgttcact aattagcacg taaaatcagt
    14341 aacggctgga aatcattcaa tactcgcact atcggaagtt caccagccag ccgcagcacg
    14401 ttcttgcata cgacgtgtct gcggtctcaa ctccgcccgc ccccgccaat catgattggg
    14461 cagtaccagt acagcactag caaagacagg aaattagcag tctcagcaaa acaccgacca
    14521 gacggtgaga agacaaaaag atacgcaaag atgccgcggt tccagaagaa taaaaaaccg
    14581 ctaatggaca gcctccttaa cacagttttt tatatacatg attcactgat accagtggca
    14641 ttaatagcta ttatatagct tcagctcctt ttttcatacc ttaaggaaaa attgccccct
    14701 cccccaaaaa gtatcgtttt tttacattaa accgatccag tttaggatag tgtaaagcag
    14761 aagttcttca atatataaca tatggagtta atatgcttac acaaactata tatccgcctc
    14821 ctccagaagt tcctgtctac tgctgaaggg aaccaataac attgcaaagc agataaaaaa
    14881 gaatccaaca cctctcaaca taaagaatac gccctgatat ttatgctccc gacaaataac
    14941 aatcacagat taatttcaaa ttcgttctcc acttattcaa tcgacactag ccgtgcatat
    15001 gaaaattatc taacccattg gactgaatgg aaaaataacc gcatacaaga agaacaacga
    15061 gacatcgctt ttcagcgact agtatcatgt ctacaaaacc aagagacgaa cctggacttg
    15121 tctgaattag gcctgacaac attacctgaa atccccccgg gaattaaatc aattaatata
    15181 agtaaaaata atttaagctt aatctcccca ttgcctgcgt cccttacaca gcttaatgtc
    15241 agctataaca gacttattga actgcctgct ttgcctcaag gacttaaatt attgaatgcg
    15301 tcccacaatc aactaatcac actacccaca ctccccatat ctttgaagga gcttcatgtc
    15361 tcaaataatc aattatgttc tcttcctgtt ttaccagaac tactggaaac attagatgta
    15421 tcatgtaatg ggctggcagt tttaccacct ttaccatttt ctttacaaga gattagcgca
    15481 atagggaatc ttcttagtga actcccccct ctacctcaca acattcactc catatgggca
    15541 atcgacaata tgttaaccga tattccatac ctgccggaaa atttaaggaa cggttatttt
    15601 gacataaatc agataagtca tatcccggaa agcattctta atctgaggaa tgaatgttca
    15661 atagatatta gtgataaccc attgtcatcc catgctctgc aatccctgca aagattaacc
    15721 tcttcgccgg actaccacgg cccgcagatt tacttctcca tgagtgacgg acaacagaat
    15781 acactccatc gccccctggc tgatgccgtg acagcatggt tcccggaaaa caaacaatct
    15841 gatgtatcac agatatggca tgcttttgaa catgaagagc a
//
LOCUS       DAESHI010000097        15543 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.098, whole genome shotgun sequence.
ACCESSION   DAESHI010000097 DAESHI010000000
VERSION     DAESHI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15543
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.098"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..357
                     /gene="mcbR"
                     /locus_tag="KW011_003150"
     CDS             <1..357
                     /gene="mcbR"
                     /locus_tag="KW011_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HBH7661423.1"
                     /translation="LTPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSN
                     MPILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLA
                     EIMQQNIAILYQQYNR"
     gene            complement(393..2495)
                     /locus_tag="KW011_003151"
     CDS             complement(393..2495)
                     /locus_tag="KW011_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBH7661424.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDIP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            2737..3798
                     /locus_tag="KW011_003152"
     CDS             2737..3798
                     /locus_tag="KW011_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBH7661425.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3913..5412)
                     /gene="ansP"
                     /locus_tag="KW011_003153"
     CDS             complement(3913..5412)
                     /gene="ansP"
                     /locus_tag="KW011_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HBH7661426.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGMPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5679..6296
                     /locus_tag="KW011_003154"
     CDS             5679..6296
                     /locus_tag="KW011_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HBH7661427.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGAGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
     gene            6372..6584
                     /locus_tag="KW011_003155"
     CDS             6372..6584
                     /locus_tag="KW011_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HBH7661428.1"
                     /translation="MLCFLIYITLPFSQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININANKNIRQVC"
     gene            7405..9513
                     /locus_tag="KW011_003156"
     CDS             7405..9513
                     /locus_tag="KW011_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBH7661429.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRI
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTLENHFAVIPADRTWRPQPLMKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIETVGVNQIETVGSNQIIKVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
     gene            9581..13123
                     /locus_tag="KW011_003157"
                     /pseudo
     CDS             9581..13123
                     /locus_tag="KW011_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            14104..15072
                     /locus_tag="KW011_003158"
                     /pseudo
     CDS             14104..15072
                     /locus_tag="KW011_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            15330..>15543
                     /locus_tag="KW011_003159"
     CDS             15330..>15543
                     /locus_tag="KW011_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661430.1"
                     /translation="MSGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPVLGAKVLPGETDLALPCPLP"
BASE COUNT         4083 a         3729 c         4197 g         3534 t
ORIGIN      
        1 ctcaccccgc aagacctgac ggaactccag gaattgctcg agaaattaca gcaggcgcaa
       61 gaaaagggcg acatggaaca aatcattaat gtaaacaggc tatttcgctt agcgatttat
      121 catcgctcaa atatgcccat cctgtgtgag atgattgagc aactgtgggt caggatgggg
      181 cctggtttac attatctcta tgaagcgatt aatccagcgg aattacggga gcatatagaa
      241 aactatcatc tgttactcgc tgcgttaaaa gcaaaagaca aagagggatg cagacattgt
      301 cttgctgaaa ttatgcaaca aaatattgct attttatacc agcaatacaa tcgttaaagt
      361 aaatgcccgg caaataccgg gcatcaaggt aattactcaa atctccacgc aatattcatg
      421 ccgacaccat aatttcgtcc gggcgaaggt tcgtaatatc gcccgtttga ctcattgaca
      481 atgacagaac caacgtattc tttatcgaat aaattatcga cacgaccaaa taaatccaca
      541 gttaaattgt ggtaattgta tttatacccg gtgaataagc cgacgagagt ataagacggc
      601 gcttttgccg tattttcatc atctgccata atatcgccca tataacgcgc ttccgtgcct
      661 gcataccaac catcttccgg tacataacct atcgacgcaa agcccatatt acgggcgatc
      721 cctggcatcc gattaccgtt acaatcctgt tcattgcaaa cattgctgcg ataggtcgca
      781 tcaagccagg tccaggacgc gtttacgcga aaatctcctg cgaaacgttg atcccatgcc
      841 agttcagcgc cttgacgacg ggtctttccg gcatttttgt aagtcgtacg cccaccgcta
      901 ctgctatcga caacaatttc atcatcagtg tcggtctgaa acaatgcgag actaagcagc
      961 ccatcaccaa tacgcgtttt actgccgatc tcaattgtat cgttggtgga tggttttaaa
     1021 ccaaagttca taccgctttg cccatcagca cgataagaca gctcattaat cgtcggcgtt
     1081 tcaaaacctc gcccggctgc cagatagata ttccaggcat cggtcattgc atattttaac
     1141 gaaccggcag gtagccattt atgataactg gcatcaccgc tgtcatcgcc gttacccgga
     1201 gtaacgtaat ggtcgttgga atcaaaccac acggagctgt agcgcacgcc agcatccagc
     1261 gacagttttt cgctcagctg ccactgcgtc tgtaaatagg gatcgatgtt ccacatcaga
     1321 ttgcgttcgt cgcgacgcaa ctcacctttt tgcccgtact ccggcatgcc gctattcagg
     1381 cggaagttat tgtagccctt gcggttttca ctcatgtttt cgtagttcag gccggtagtg
     1441 aacgtgaccg gaacgcccag ttcaccacgg tgtgtccagc ggctgtctat cccctggtaa
     1501 tggcgttgca gggtaatcac gccgcccgca tgtgacgggt taagttgtgg tgccatgggt
     1561 attgactggt actgggtcgt ttctcgctct ccggcataca tcatcacact catatcatct
     1621 tgcgcgctca ggctacgctc atagcgcaac ccagcctgag tttgcttgat ggtttttcgc
     1681 gtgtcgtact gttctgcacg aggcgcttgt tgtggattag ccttccattc tgctttggtt
     1741 agcccacccg ggtcatctgc tttgatatcc acactattga aaatcagact taatttgctg
     1801 gcttcatcaa tgcgtacgcc cagtttggca ttggctaaat ttttctgtgc gccactatgg
     1861 tcacgatagc cgtgggtcgt aaaacgcgtg gttgagacgg tgtaatcgac atcgccaggc
     1921 tgtgtgccgt ctcccgttgc gcccgttgct ttcagcccat agcgccagct gccaaaactg
     1981 ccgtagtaac tactggcttc aatggttggt ggctgttgtc cggtctgggt ggtgacattc
     2041 attaccccac cagacgcgtt gccatacagg gcagagaagg ggccacgcag cacttccaca
     2101 ttttgcacac tgcttaaatc gatgttggat gtttgccctt gcccgtcggg catggtggcg
     2161 ggaataccgt ccacatacag gcgaataccg cgaataccgt aagtggagcg ggagccaaat
     2221 ccgcgaatcg acagctgtaa atcttgcgca tagttctgcc ggttttgtac ctgcaaacca
     2281 ggcacgtcgg tcagtgattc ggacaagtta atgcgcggtg ttgccaggcg catctcctcg
     2341 ccatccacca cgcttactgc tgctgggata tccagttctg aaaccacctg cggtgcggca
     2401 ctgacaatca tggtctgttc atcagcggca aaaacaacgg gggaaagggc aagtagtgcg
     2461 ggcaaaacgg tctgtcggac ggaaaaaatc ttcatgaaaa aagccaggtt aagaatggga
     2521 aaacgccgtt atggtaataa aattgtaaat tcatggaaaa cgaaacggca cgatacgtta
     2581 agtaattgag aaaattgtag tcgtaacggc aagaaatgct ccacatttga gaaaataatg
     2641 attaccattc ccatttataa caagagcgta acgatgatta cgcttagcga agcattgtga
     2701 agcagcaaaa atatcggttc atcaaaggga gtcgtcatgc atttacgtca tctgttttca
     2761 tcgcgcctgc gtggttcatt actgttaggt tcattgcttg ttgcttcatc attcagtacg
     2821 caggccgcag aagaaatgct gcgtaaagcg gtaggtaaag gtgcctacga aatggcttat
     2881 agccagcaag aaaacgcgct gtggctcgcc acttcgcaaa gccgcaaact ggataaaggc
     2941 ggcgtggttt atcgtcttga tccggtcact ctggaagtga cgcaggcgat ccataacgat
     3001 ctcaagccgt ttggtgtcac catcaataac acgactcaga cactgtggtt tggtaacact
     3061 gtaaacagcg cggtcacggc gatagatgcc aaaacgggcg aggtgaaagg ccgtctggtg
     3121 ctggatgatc gtaagcgtac ggaagaggtg cgcccgctgc aaccgcgtga gctggtagct
     3181 gacgatgcca cgaacaccgt ttacatcagt ggtattggta aagagagcgt gatttgggtc
     3241 gttgatggcg agaatatcaa actgaaaacc gccatccaga acaccggtaa aatgagtacc
     3301 ggtctggcgc ttgatagcaa aggcaaacgt ctttacacca ctaacgctga cggcgaattg
     3361 attaccatcg acaccgccga caataaaatc ctcagccgta aaaagctgct ggatgacggc
     3421 aaagagcact tctttatcaa cattagcctt gataccgcca ggcagcgtgc atttatcacc
     3481 gattctaaag ccgcagaagt gttagtggtc gatacccgta atggcaatat tctggcgaag
     3541 gttgcagcac cggaatcact ggctgtgctg tttaacccag cgcgtaatga agcctacgta
     3601 acgcatcgtc aggcaggtaa agtcagtgtg attgacgcga aaagctataa agtagtgaaa
     3661 acgttcgata cgccgactca tccaaacagc ctggcgctgt ctgccgatgg caaaacgctg
     3721 tatgtcagtg tgaaacaaaa atccactaaa cagcaggaag ctacccagcc agacgatgtg
     3781 attcgtattg cgctgtaata gatatggcga gggtgaaaac ggagagactt gcctgacttt
     3841 tatcacgccg catgcggcac accttcgtca gtaatctaaa tttgcccggc acgtagccgg
     3901 gcaattttgc aattagctcg ccgtttcagg cttaaacaca atttcctgtt tttcttcatc
     3961 ttcctcgacg actggcgcag tgctgtgaat ttcagcaacg cgtttgcgca caccaaacca
     4021 gcctataacc agcagaatac cgataatcgg cagcgccgcg atagtataag tcccgttcgg
     4081 gtaatcgaac gccatcagca caaggacact cagtaaaaac agtaatgtca gccaggaagt
     4141 gaagggcgcg ccaggcagtt taaaactgac atccgctgct ttgccttctt taatcgcttt
     4201 acgcaggcgc atctggcaca cgatgataaa cgcccatgaa gcgatgattc ccagcgacgc
     4261 gaagttcaac acaatctcaa atacgcgcga cggcaccaga tagttgagga atacgccgac
     4321 gacatacaca accagtgtcg ccagaatccc ggcatacggc acatgctgac gactcatttt
     4381 cgccataaaa ctcggtgcgg aaccgcccat cgccatcgag cgcagaatac gcccggtgca
     4441 gtacagacct gagttcaggc tggagagggc agcggtcagc accacaatgt tcataatgct
     4501 gccgatatat ggcataccca gtttagagaa aaacgtcacg aacggacttt gccccgcctg
     4561 atacgcgctc cacggcaata acataaccag caacaccacg gagccgacgt aaaacaggcc
     4621 aatacgccaa atcacactgt taatggcttt aggcaccatg gtctgcggat ctttacattc
     4681 tccggcagct gtacccacca tttcaatgga ggcaaaagca aacactacgc cctgaatcaa
     4741 caccagcgca ggcagcaaac cgtgggggaa gaagccgcca ttatcggtga ttaaatgaaa
     4801 gccagtggtg ttgccatcca gcggctgacc actaccgagg aacactgtac ccacgaccaa
     4861 aaaggtcaca atggcgagca ctttaataag cgcaaaccag aactccatct ccgcaaacca
     4921 tttcacaccg atcatattca tggtgccaac gatggtaagt gcagcgagcg caaagaccca
     4981 ctgcggcacg ccgccaaacg caccccagta atgcatatac agagcgacgg cggtaatatc
     5041 aacaatcccc gtcatcgccc agttgatgaa gtacatccag ccagcaacat aagcggcttt
     5101 ctcacccaaa aactcacggg cataagagac aaaactgcca ctggaagggc ggtgtagcac
     5161 cagctcaccc aatgcacgca gaataaaaaa cgaaaacaag ccacaaatta aataaaccag
     5221 tgccagtgct ggacccgcca tttgcagtcg ggctcctgca cctaaaaaca agccggtgcc
     5281 aatcgcgccg ccaatggcga tcatctgtac ctggcgattg cccatcgctt tgtgataccc
     5341 ctcttcgtgg gcattaagcc agcggcgttt cgcggcgtgt tgatctgaag tgtcggtgtc
     5401 gtgtttactc attgctctcc ctgattgctt taatgaaaaa gtcatataag ttgccatgaa
     5461 caatgtttat tcatcctggc gatgatagtt atcaccatta ttctttatgg gtataaaact
     5521 atcggcgcag catcctaccc gttatcgata tacgatgcaa aacatcccct tacaatcctg
     5581 aaggggattc atacaactga cgaaaaaatg acaaatactt ttgctggtta acctgtgtac
     5641 tgtcctacac ttaatcttta aaagattgtg aggggcatat gattaaggtg tatggcgtac
     5701 ccggatgggg ctctacaatc agtgagctga tgctaacact ggctgatatt ccttatcagt
     5761 tcgttgatgt cagtggtttt gaccacgagg gaacttcacg cgagttattg aaaaccctga
     5821 acccgttgtg tcagattccg acactggcgc tggaaaatga cgagattatg acggagacag
     5881 cggcgattgc attgatggtt ctcgatcgtc gcccggacct tgcgccaccg gttgggcgcg
     5941 ccgaacgtca gcagtttcaa cggctattgg tctggctggt tgctaatgtc tatccaacat
     6001 tcactttcgc cgattaccct gagcgttggg ctcctgacgc acctgaacag ttaaagaaga
     6061 atgttattga atatcgtaaa tcgctttata tctggctgaa ttcacaattg accgctgaac
     6121 cttatgcgtt tggtgagcaa ctaacgctgg tggactgtta tctttgcact atgcgcacat
     6181 ggggggctgg gcatgaatgg tttcaggata atgccacgaa tatcagtgcg attgctgatg
     6241 ctgtatgtca gttaccgaaa ttacaagaag tattaaaaag gaatgaaatt atttgagtgg
     6301 gataccagag gattatgtta tttattattc agtaacttta tccacaatga tgggcgtaat
     6361 taattaaatt catgttatgt tttttgattt atatcactct ccccttcagt cagcttgtct
     6421 atttcatttc ctctgaaaag aaactaacta ttcacattgt gcagatgttt cacttgttat
     6481 cacaggtatt ctataatctc aaaaagtttt taatgatgga tatgctcgga gttggagatg
     6541 caattaatat taatgcaaat aaaaatatcc ggcaggtatg ctaaaaggat aaatggcgtt
     6601 ctctgtattt attcgcaatt atctatagga caaatatgga gagcttgatg agtcagagct
     6661 aaaacagcaa ttgataaata aatatgttga tttattcaag aaaatggatg tattttaaat
     6721 attgattctt aatataacct aatcaataga ttatcacgtg agagctgcac attattggcg
     6781 gggccgggga aaacatcatt ttgggaacaa tgaatcaggt actcttacca ctgaagcaag
     6841 atcatccggg caccaggtgg tggtgcagtg ctttggaaat ggtcctctcg gggggagttg
     6901 ccattatttt ctatgctatt tgcttattat attcatcagg tgaatttaat aaattcctaa
     6961 tttgaaatat ccactattaa gccagtgttt aacgacgtta agatggcaat gtgcagatca
     7021 tgttatcagg gtgggcggta gcgtatattc cttatacaaa ccatcattca tttcaatgat
     7081 tgttattgaa ttatttattg ttgtgaataa aaagacatta tgaatattgg tttgactttg
     7141 catatcaggt tttttattac tcgctgtaat gtacgagcct gtcgtgaata acgtcgagaa
     7201 cccatccgtt gcgtttgtga ttattttgtt gactaaacag acacccgttt ctctgaagta
     7261 aaatcccaga ctaaatcatc acataaccat gacatttttc tgatattccc cggtaacgcc
     7321 agatgtcgac tcgcttatct catccgcccg acatgttgta catgcaatat gtgcggatta
     7381 ttattcattt tcacggaggt tgctatgtca accggattac gtttcacact ggaagtggac
     7441 ggcctgccac cggatgcttt tgcggtggtt tcctttcatt tgaaccagtc actctcttcg
     7501 ctcttttccc tcgatctctc tctggtcagc cagcagtttc tctcccttga atttgcgcag
     7561 gtgctggaca aaatggccta cctgacggta tggcagggcg atgacgtaca gcgccgggtg
     7621 aaaggtgtgg tgacctggtt tgaactgggg gagaacgaca aaaaccagat gctgtacagc
     7681 atgaaggtat gcccaccgct gtggcgcata gggctgcgcc agaacttccg tatcttccag
     7741 aatgaggaca tcgaaagcat cctcgcgacg atcctgaagg aaaacggtgt gaccgagtgg
     7801 agtccgctgt tcagcgagcc gcatccttcc cgtgagtttt gtgtccagta cggcgaaact
     7861 gattacgatt tcctgtgccg gatggcggcg gaggaaggca tcttctttta tgaggagcac
     7921 gcgcaaaaaa gtaccgacca gagcctggtc ctgtgcgaca ccgtgcgtta tctgccggag
     7981 tcctttgaga tcccctggaa cccgaacacc cgtaccgagg taagtaccct ctgcatcagc
     8041 cagtttcgct acagcgcaca aatccgtcct tcttccgtag tgaccaaaga ctacaccttt
     8101 aaacgtcccg gctggccagg gcgttttgat caggaaggcc agtaccagga ttaccagcgc
     8161 acgcagtatg aagtgtatga ctaccccgga cgtttcaagg gtgcccacgg gcagaacttt
     8221 gcccgctggc agatggacgg ctggcgaaac aacgcagaag tggcgcgcgg aacaagccgt
     8281 tcgccggaga tatggccggg acggcgaatt gtgctgacgg ggcatccgca ggcgaacctg
     8341 aaccgggaat ggcaggtggt ggcgagcgat cttcacggcg aacagccgca ggcggtaccg
     8401 ggacgcaggg gttcaggcac cacgctggag aaccattttg cggtaatccc ggcagacaga
     8461 acatggcggc cacagccgtt gatgaaaccg ctggtggatg gcccgcagag cgccgtcgtg
     8521 acgggaccgg caggcgagga aatcttctgt gatgaacatg gccgcgtgcg ggtgaaattt
     8581 aactgggacc gctataaccc gtcaaaccag gacagttcat gctggatccg tgtggcacag
     8641 gcgtgggcag gcaccggctt tggcaacctg gcgataccgc gtgtgggtca ggaggtgatt
     8701 gtggacttcc tcaacggcga cccggaccag ccgattatta tggggcgtac ctaccaccag
     8761 gaaaaccgca cacccggcag cctgccgggg acgaagacgc agatgaccat tcgttcgaaa
     8821 acctataagg gcagcgggtt taatgaactg aagtttgacg atgcgacagg gaaagaacag
     8881 gtctacatcc acgcgcagaa gaacatgaac accgaggtgc tgaataaccg caccacggat
     8941 gtgataaaca accatgctga aaccattggc aacaatcaga tgattgcggt taccaacaat
     9001 cagatagaga cggtgggcgt taaccagata gagacggtgg gcagtaacca gataattaaa
     9061 gtgggttctg ttcaggtgga aacgattgga cttgttcgtg cgctgacggt gggcgtggcg
     9121 tatcagacga cggtaggtgg cattatgaac acctcggtgg cactgatgca gtcctcgcag
     9181 atcggtttgc ataaatcgtt gagggtcggg ctgggttatg acgtcaaagt cggaaataac
     9241 gttaccttca ccgttggtaa aacgaaaaag gatgataccg ggcagaccgc gatttactcc
     9301 gccggtgagc atctggagct ctgctgtggt aaggcaaggc tggtgctgac gaaggacgga
     9361 caaatttttc tcaacggcac aaaaattcat ttgcagggta aggagcaggt taatggtgac
     9421 tcactgttga ttaactggaa ctgtgcagcc tcgaaatccc caccgaagac ccctgatgaa
     9481 aagcaggata cgccggatat gagagagtac tgatcccgta cctgttcggt attcagtaac
     9541 aaaccagcat taaaaataaa cagaagaagg aatttgaatc atgagcggaa aaccagcggc
     9601 gcgtcaggga gatatgactc agtatggcgg tcccattgtc cagggttcgg caggtgtaag
     9661 aattggcgcg cccaccggcg tggcgtgctc ggtgtgtccg ggcgggatga cttcgggcaa
     9721 cccggtaaat ccgctgctgg gggcgaaggt gctgcccggc gagacggacc ttgcgctgcc
     9781 cggcccgctg ccgttcattc tctcccgcac ctacagcagc taccggacga agacgcctgc
     9841 accggtgggc gttttcggcc ccggctggaa agcgccttct gatatccgct tacagctacg
     9901 tgatgacgga ctgatactca acgacaacgg cgggcggagc attcactttg agccgctgct
     9961 gccgggggag gcggtgtaca gccgcagtga gtcaatgtgg ctggtgcgcg gtggtaaggc
    10021 agcacagccg gacggccata cgctggcgcg gctgtggggg gcgctgcgcc ggatatccgg
    10081 ttaagcccgc atctttacct ggcgaccaac agcgcacagg ggccgtggtg gatactgggg
    10141 ggggctgagc gggtgccggg tgctgaggac gtactgccag cgccgctgcc gccgtaccgg
    10201 gtgcttaccg ggatggcgga cgctgacgta ccggcgtgag gccgccggtg acctggccgg
    10261 ggaaatcacc gacgtgacgg acggtgccgg gcgggagttc cgtctggtgc tgaccacgca
    10321 ggcgcagcgt gcggaagagg cccgcacctc ttcgctatct tcttctgaca gttcccgccc
    10381 tctctcagcc tcagcgttcc ctgacacact gcctggtacc gaatacggcc ccgacagggg
    10441 tatccgcctt tcggcggtgt ggttgatgca cgacccggca tacccggaga gcctgcccgc
    10501 tgcgccactg gtgcggtaca cgtatacgga agccggtgaa ctgctggcgg tatatgaccg
    10561 cagcaatacg caggtgcgcg ctttcacgta tgacgcgcag cacccgggcc ggatggtggc
    10621 gcaccgttac gcgggaaggc cggagatgcg ctaccgctac gacgatacgg ggcgggtggt
    10681 ggagcaactg aacccggcag ggttaagcta ccgctatctt tatgagcagg accgcatcac
    10741 cgtcaccgac agcctgaacc ggcgtgaggt gctgcataca gaaggcgggg ccgggctgaa
    10801 acgggtggtg aaaaaagaac tggcggacgg cagcgtcacg cgcagcgggt atgacgcggc
    10861 aggaaggctc acggcgcaga cggacgcggc gggacggcgg acagagtacg gtctgaatgt
    10921 ggtgtccggc gatatcacgg acatcaccac accggacggg cgggagacga aattttacta
    10981 taacgacggg aaccagctga cggcggtggt gtacccggac gggctggaga gccgccggga
    11041 atatgatgaa ccgggcaggc tggtatcgga gacatcgcgc agcggggaga cagtacgcta
    11101 ccgctacgat gacgcgcaca gtgagttacc ggcgacgaca acggatgcga cgggcagcac
    11161 ccggcagatg acgtggagcc gctacgggca gttgctggcg ttcactgact gctcgggcta
    11221 ccagacccgc tatgaatacg accgcttcgg ccagatgacg gcggttcacc gcgaggaagg
    11281 tatcagcctt taccgccact atgacaaccg tggccggtta acctcggtga aagacgcaca
    11341 gggccgtgaa acgcagtatg agtacaacgc cgcaggcgac ctgactgccg ttatcacccc
    11401 ggacggcaac cggagcgaga cacagtacga tgcgtgggga aaggcggtca gcaccacgca
    11461 gggcgggctg acgcgcagta tggagtacga tgctgccgga cgtgtcatca gtctgactaa
    11521 cgagaacggc agtcacagcg acttcagcta cgatgcgctg gaccggctgg tacagcaggg
    11581 cggctttgac gggcggacgc aacgttatca ttatgacctg accggaaaac tcacgcagag
    11641 tgaggatgag gggcttgtca ccctctggca ctacgatgcg tcggaccgca tcacgcaccg
    11701 gacggtgaac ggcgacccgg cagagcagtg gcagtatgac ggccacggct ggctgagaga
    11761 aatcagccac ctgagcgaag gccaccgtgt cgctgtccac tacggctacg acgataaggg
    11821 ccgcctgacg ggcgaacggc agacggtgga gaacccggag acgggggaac tgctgtggca
    11881 gcatgagacg aaacacgcct acaacgaaca ggggctggca aaccgcgtca cgccggacag
    11941 cctgccgccg gtggagtggc tgacctacgg cagcggttac ctggcgggca tgaagctggg
    12001 cgggacgccg ctggtcgagt atacgcggga caggctgcac cgtgagacgg tgcgcaccct
    12061 cgcaccggac ctggacatcc gcatcccgta tgccaccgac ccggcgggaa accgcctgcc
    12121 ggacccggag ctacacccgg acagcacgct cacggcgtgg ccggataacc gcatcgcaga
    12181 ggatgcgcac tatgtttacc actatgatga atacggcaga ctgacggaga agacggaccg
    12241 catcccgacg ggtgtgatac ggacggacga cgagcggacc caccactacg acagccagca
    12301 ccgcctggtg ttccacacgc ggatacagca tggcgagcca ctggtcgaga gccgctacct
    12361 ctacgacccg ctgggacggc gaatggcgaa acgggtatgg cggcgggagc gtgacctgac
    12421 ggggtggatg tcgctgtcgc gtaaaccgga ggagacatgg tacggctggg acggcgacag
    12481 gctgacgacg gtacagaccg acaccacacg tatccagacg gtatacgagc cgggaagctt
    12541 cacgccgctc atccgggtcg agacagagaa cggcgagcgg gagaaagcgc agcgccgcag
    12601 cctggcggag aagctgcagc aggaagggag cgaggacggg cacggcgtgg tatttccggc
    12661 tgaactggtg cggctgctgg acagactgga ggaagaaatc cgggcagacc gcgtgagcag
    12721 tgaaagccgg gtgtggcttg cgcagtgcgg gctgacggtg gagcaactgg ccagacaggt
    12781 ggagccggaa tacacaccag tgcgaaacgt tcatctttac cactgcgacc atcggggcca
    12841 gccgctggcg ctcatcagcg aagacggcga tacggtgtgg agcggggcgt atgatgagtg
    12901 gggcaaccag ctaaatgagg agaacccgca tcatgtgtat cagccatacc gtctgccggg
    12961 gcagcagtat gatgaggagt cggggctgta ctacaatcgt caccggtact atgatccgtt
    13021 gcagagacgg tacatcaccc aggaccccat tgggctgaaa ggtgggtgga atctgtatca
    13081 gtatccgctg aatccggtga gcaggataga tccgttgggg ttattctcat gtgagagttg
    13141 tagtcatttt atagatgata taaataataa actggataac cttccgttta acccgacaaa
    13201 aatgtttaca gctagtttaa atactgctaa ttcagcaagg ctgtatgcaa gtggcatgac
    13261 aaaattcggt ataagtgttg ctgccgatgg aacgatcatt gctgccccag tcggtgcagc
    13321 ttctatggct ttaggggcgt ggaatataaa atctgcgaca gctgcatata acagagcaaa
    13381 tttacaatta tcagaatcac ttcaggaaaa tggtagagat aggtgttgga gaaatttatt
    13441 gggtatttta ccttatggtg agcagtttga tgaccctggt gaaccatcaa tattagacgt
    13501 ttatggtgaa aagattgaga atatcaaaga ttctccaatg aagttttttg aagagcttgg
    13561 gacgctgggg ttgtaatgtg tatttttaat gatattttta tgtttgtgtt tattgttagt
    13621 agtatacttg taaccgtgca ttttgtgtat tctttatttt ttaaaagtgg aaaggtaaaa
    13681 gataaggtaa acaaatggat taaagtaatg tgtgatttat tttgggggat aggatagtgg
    13741 tgtgatatgg ataaatataa attacagtgg ctggcatatt tcgacctgac gccggagtag
    13801 ataaaaaaac agacaggatt ggagtatagc aagcgtctga caatctctcg ggacagtagt
    13861 atgatgaaga ggcggggttg tactataacc gtaaccggta ctacgatccg ctaaagggca
    13921 agtatatgac tcagggaaag atcatcagaa gttcactttt tgtactaaat aattcgcatt
    13981 ttatgtttaa aaattgagat attcctcatt acctgaagct gtttttttat tgcttataca
    14041 taatcaaata ctccttacat aattaaggag aacaaaatgg aaattaaaaa attgatggaa
    14101 cattttttga agcaatatgg tgattttgaa aatggcattc ctgttcacga taccattgcc
    14161 agagttgtat cctgtatcag tcctgcaaaa tttcatgagt gctttattaa ctggatgcgt
    14221 gactgccatt cttcagatga taaagacgtc attgcaattg atggaaaaac gctccggcac
    14281 tcttatgata agagtcgtcg caggggagcg attcatgtca ttagtgcgtt ctcaacaatg
    14341 tacagtctgg tcctcgggca gatcaagacg gatgagaaat ccaatgagat tacagccatt
    14401 cctgaacttc ttaacatgct ggatattaaa ggaaaaatca tcacaactga tgcgatgggt
    14461 tgccagaaag atattgcaga gaagatacaa aaacagggag gtgattattt attcgctgta
    14521 aaaggaaacc aggggcggct aaataaagcc tttgaggaaa aatttccgct gaaagaatta
    14581 aataatccag agcatgacag ttacgcaatc agtgaaaaga gtcacggcag agaagaaatc
    14641 cgtcttcata ttgtttgcga tgtccctgat gaacttattg atttcacgtt tgaatggaaa
    14701 ggactgaaga aattatgcgt ggcagtctcc tttcggtcaa taatagcaga acaaaagaaa
    14761 gagccagaaa tgacggtcag atattatatc agttctgctg atttaactgc agaaaagttc
    14821 gccacagcaa tccgaaacca ctggcacgtg gagaataagc tgcactggcg tctggacgtg
    14881 gtaatgaatg aagacgactg caaaataaga agaggaaatg cagcagaatt attttcaggg
    14941 atacggcaca ttactattaa tattttgacg aatgataagg tattcaaggc agggttaaga
    15001 cgtaagatgc gaaaagcagc catggacaga aactatctgg cgtcagtcct tgcggggagc
    15061 gggctttcgt aatcttgccc tgctgaagag tttgaaaggg cacggatcct ttcaaaagaa
    15121 tacattgaga aatatataag ctaaagatga ttagctgtat ttcatgataa taatctaatg
    15181 agggattttt ctgaacatca aattgcatcg aagttagtta gagtgctgcg attgtcttgc
    15241 acgtaataaa ttcagagaga aaatatagtt gtgggtatga gaaaataacc aacctaatgt
    15301 aaatcaattc agatgaagag gaataaacca tgagcggaaa accggcggcg cggcagggcg
    15361 acatgacccg caagggactg gatattgtgc agggttcagc gggtgtgctg ataggtgcgc
    15421 cgacgggagt ggcctgttcg gtgtgtccgg gagggattac ctatgctaac ccggtgaacc
    15481 cggtgctggg tgcgaaggtg ctgccgggcg agacggacct tgcgctgccc tgcccgctgc
    15541 cgt
//
LOCUS       DAESHI010000098        15511 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.099, whole genome shotgun sequence.
ACCESSION   DAESHI010000098 DAESHI010000000
VERSION     DAESHI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15511
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.099"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            202..474
                     /locus_tag="KW011_003160"
     CDS             202..474
                     /locus_tag="KW011_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HBH7661431.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            567..650
                     /locus_tag="KW011_003161"
     CDS             567..650
                     /locus_tag="KW011_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661432.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            750..1571
                     /locus_tag="KW011_003162"
     CDS             750..1571
                     /locus_tag="KW011_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HBH7661433.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="KW011_003163"
     CDS             complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="KW011_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HBH7661434.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="KW011_003164"
     CDS             complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="KW011_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HBH7661435.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2703..3737
                     /gene="tqsA"
                     /locus_tag="KW011_003165"
     CDS             2703..3737
                     /gene="tqsA"
                     /locus_tag="KW011_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HBH7661436.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="KW011_003166"
     CDS             complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="KW011_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HBH7661437.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPAAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="KW011_003167"
     CDS             complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="KW011_003167"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HBH7661438.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7217..8161
                     /locus_tag="KW011_003168"
     CDS             7217..8161
                     /locus_tag="KW011_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBH7661439.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8347..9729
                     /locus_tag="KW011_003169"
     CDS             8347..9729
                     /locus_tag="KW011_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH7661440.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9766..10488
                     /gene="folM"
                     /locus_tag="KW011_003170"
     CDS             9766..10488
                     /gene="folM"
                     /locus_tag="KW011_003170"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HBH7661441.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10485..10820)
                     /locus_tag="KW011_003171"
     CDS             complement(10485..10820)
                     /locus_tag="KW011_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HBH7661442.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10949..11668
                     /gene="rstA"
                     /locus_tag="KW011_003172"
     CDS             10949..11668
                     /gene="rstA"
                     /locus_tag="KW011_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HBH7661443.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11672..12973
                     /gene="rstB"
                     /locus_tag="KW011_003173"
     CDS             11672..12973
                     /gene="rstB"
                     /locus_tag="KW011_003173"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HBH7661444.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            13049..13978
                     /gene="tus"
                     /locus_tag="KW011_003174"
     CDS             13049..13978
                     /gene="tus"
                     /locus_tag="KW011_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HBH7661445.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="KW011_003175"
     CDS             complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="KW011_003175"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HBH7661446.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
BASE COUNT         3811 a         4021 c         3794 g         3885 t
ORIGIN      
        1 ggttatcgct cgccacggga atatctgcgg cagcgggctt gtaatgggtt aagtgataac
       61 agatgtctgg aaatataggg gcaaatccac tgccattccc agggatatag ttatttcaac
      121 ggccccgcgg tggggttaaa tgaaaaaaca aattgagggt atgacaatga aaaagtatta
      181 gctctggttg ttgccgctgc tatgggtctg tcttctgccg cctttgctgc agagactgcg
      241 accacacctg ctccgactgc gacgaccacc aaagcagcgc cggcgaaaac gacacatcat
      301 aaaaaacagc ataaagcagc acctgcccag aaagcgcagg cggctaaaaa gcatcataaa
      361 aatacgaaag ctgaacagaa agcccctgaa caaaaagcgc aggcagcgaa gaaacacgcc
      421 gggaaacaca gccatcagca accggcaaaa cctgctgcac aacccgcagc gtaagttttc
      481 aacagtaatg ctggcgcgcc ccctcgcgcc tgaaaattac ggtgctaagc gggtaacgtt
      541 tagcaccgcc tttagcggag ggtagtatgg tgggccgtta tcgctttgag ttcattctta
      601 tcatccttat tttatgcgca ctgattgccg cccgttttta tctttcctga ctgtagttat
      661 ctgattttac tcccactttc atcccgtccc gtctatagta tttatgaggg tttgctttta
      721 ataatcataa ttacccacca gagtgtgata tgcgtacaac cattgctgta gtgttgggtg
      781 caattagttt gacgtctgct tttgtgtttg cagataaacc agacgttgcc aaatcggcaa
      841 acgatgaggt cagcaccctg ttttttggtc atgatgatcg tgtgccagtg aatgacacga
      901 cccaatcacc gtgggatgcg gttgggcaac tggaaacggc cagcggcaat ttatgtacgg
      961 cgacgctgat tgcacccaat ctggcattaa cggcaggaca ctgtttattg acacctccaa
     1021 agggtaaagc cgataaagca gtggcgctgc gttttgtgtc aaataaaggt ctttggcgct
     1081 atgagatcca cgacatagaa ggccgcgttg atccgacact gggaaagcgg ttaaaagcag
     1141 atggggatgg ttggattgta cctcccgcag ccgcgccgtg ggacttcgga ttgattgtgc
     1201 tacgtaatcc cccttctggc attacaccgt tgccgttatt tgagggagat aaagccgcgc
     1261 ttactgccgc attaaaatcg gctggccgta aagtaactca ggcgggatac cctgaagatc
     1321 atctcgatac gttgtacagt catcaaaact gtgaagtgac tggctgggcg caaacgtcgg
     1381 tgatgtcaca tcagtgcgat accttgccgg gtgacagcgg ttcgcctctg atgttgcata
     1441 ccaatgacgg ctggcaatta attggggtgc aaagttcggc tcctgccgcg aaagatcgct
     1501 ggcgcgccga taaccgggcc atttctgtta ccggttttcg cgacaagctg gatcaactgt
     1561 cgcaaaaata atgttcaaac gctgcccgac tgtgcgggca gcgtgttcat caggcaagtt
     1621 tcaccatgat cattccagcc aacagcaaga ccaggccaat ccagccttta cgatttaacc
     1681 gttgaccaaa caagatccaa cctgcggcta acgtggcggc aataccaaac ccgccccaca
     1741 atgcataagc gacagacaag tcgatccctt taacggcctg agaaagcgca ctaaaggcag
     1801 ccagcaccgc cgccagggag agcaagccaa atattttgcg acgaaagccg tcagaaaatt
     1861 tcaaaaagac gttagcaacg atttccagca cgattgccaa tgccagccag gcggcgtgaa
     1921 cccattcaaa ctgcgccatg gttcacctcc agttcaggtt tacgcgcttt acgggtacct
     1981 gattttatca acacaatccc ggcgaccagg gtggttaacc cggcaatttt catcagcgat
     2041 aaactttcgt cgaataacaa aacgctaaac aaggtaataa ataaaatacc gataccttcc
     2101 cacagcgcat aagctacgcc taaggcgatc tttttaacgg cgaaagagag aaatatatac
     2161 gacagagaaa tcatcaccag cattaaaata aagccgccat ttccctcact gacgctcgcc
     2221 catttcattg acagcgtacc ggtaatttct gtagcaatag ccaggcctaa taaaatccaa
     2281 taaatataca ttgtccttct cctgcaagag aattatttta attttcgctt aattcagcga
     2341 aaccaaagta aagtggtcaa gccccgcgca tagggcaagc ttaggcagaa gaaaggacta
     2401 aagcgcgttg cgccagtgct gctcacctac gagcaagata gtggatgagg tacgaagatg
     2461 ggtaaatgtt gaaaataacg tcctgaacaa attgtccata atattacaat tatccgcagt
     2521 gttgcttctc gtcatcgcgg atgataattg tcctcggtag ttgaacacgc ctgatttgta
     2581 tcatagctta agaattaact caaaatattt tcacttcttt acctgagcgg tttgattttc
     2641 gttatgatga cggagcgaaa aagacattat tattagcaaa ggaagaaaaa acggggacaa
     2701 gcatggcaaa gccgatcatc acgctcaatg gcctaaaaat cgtcattatg ttgggaatgc
     2761 tggtcattat tctctgcggt atccgttttg ccgccgagat catcgtgccg tttattctcg
     2821 cattatttat tgctgttatt cttaacccgc tggtgcaaca catggtccgc tggcgagtgc
     2881 cgcgtgtact ggcggtgtcg attttgatga ccatcatcgt gatggcgatg gtgttgctat
     2941 tagcttatct gggttccgcg ctcaacgagt tgacgcggac gttaccgcaa tatcgcaact
     3001 ctattatgac gccgctgcaa gctcttgagc cgttgttgca acgcgtaggg attgacgtct
     3061 cagttgacca gctggcgcat tacattgatc cgaacgcggc gatgacgttg ctcaccaact
     3121 tattgacgca gttatctaat gccatgtcat caatattttt attgctgctg acggtgctgt
     3181 ttatgctgct cgaagtgcca caattgcccg gaaaatttca gcaaatgatg gcgcgtccgg
     3241 ttgaagggat ggcggcgatt caacgtgcaa ttgacagtgt ttctcattat ctggtgctga
     3301 aaacagccat cagcatcatc accggcctgg tcgcctgggc gatgctcgcc gcactcgatg
     3361 ttcgcttcgc ttttgtctgg ggattgctgg cctttgcgct taattacatc cctaatattg
     3421 gttcagttct cgcggcaatc ccccctatcg ctcaggtact ggtgtttaat ggcttctacg
     3481 aagcgttgct ggtgctggcg ggatatctgc tgattaatct ggtcttcggc aatattctgg
     3541 agccgcgcat catgggacgt gggctggggc tttccacatt ggtggtattt ttgtcgttga
     3601 ttttttgggg atggttgtta ggaccggtgg gtatgctgct ttccgtgccg ttaacaatta
     3661 ttgtcaaaat tgcgcttgaa caaacagcgg gaggtcaaag catcgccgtt ctgttaagcg
     3721 atctcaataa agagtgacgg cctccgcaga ggccgtcagg gttacagagc tttcaggatt
     3781 gcatccacgc tggctttggc gtcaccaaac agcatgtggg tgttttcctt gaagaacagc
     3841 gggttttgca caccagcata gccagtgttc atcgaacgtt taaagacaat cacgttctgc
     3901 gctttccaca cttccagcac aggcatacca gcaataggac tcttcggatc atcctgcgcc
     3961 gccgggttaa ccgtatcgtt agcaccaatc accagtacgg tatcggtatc agcaaagtca
     4021 tcgttgatct cgtccatttc cagcacgatg tcatacggta cttttgcttc agccagcaat
     4081 acgttcatat gtccaggcaa acgccccgca accgggtgga taccgaaacg cacgttgata
     4141 ccacgagcgc gcagtttctc ggtaatttca gcggcaggat attgcgcctg cgcgactgcc
     4201 atgccgtacc ccggagtaat gatcactgaa tgggagtttt tcagcagttc cgctgtctct
     4261 tctgcggtga tttcgcggtg ctcgcccact tcctgatcat cgccagtaga agagccgtcg
     4321 gtgccgaaac cacccgcaat aacgctgata aaggaacggt tcatcgcctt acacataatg
     4381 taagaaagga tagcccccga agaaccgacc agcgcaccgg tcacaatcag caggtcgttg
     4441 ctgagcataa agcccgcagc cgcagccgcc cagccggagt acgagttcag catcgacacc
     4501 accactggca tatctgcacc accgatggag gcgactaaat gccagccgaa taccagcgca
     4561 atggcggtca ttattagcaa tgccagcact tgcaggccga cgctgtccgt gcgaacaaat
     4621 acaatcagca gcaggaagga aacgaccaga gccgccaggt tcattttgtg acggtttggc
     4681 agcatcaacg gtttagacga aatcttgcca cacagtttgc cgaacgccac caccgaaccc
     4741 gtgaacgtta ccgccccgat gaagataccg aggaacactt ccgtcaggtg aatattgacc
     4801 agaatcggtg ccattcccgc gtcatgatgc agatagctgt taaagccaac cagcactgcc
     4861 gccagaccca cgaagctgtg caggatcgcc accagttctg gcatttcggt catttcaact
     4921 ttcttcgcca gacggatacc aattgcccca ccaatgacca tcgccagcaa gatccagcca
     4981 acattacccg tatccggtcc aaaaatggtt gcgattaacg caatcgccat cccggcgata
     5041 ccgaagttgt taccctggcg agacgtttca tgtttcgaaa gaccggccag actgaagata
     5101 aacaggatcg cggcaacaat gtatgcagct gtaactaatc ctccagacat atgttacccc
     5161 ttaatttttg cggaacattt tcagcatgcg ctgagtcacg gtgaagccac cgaaaatatt
     5221 aatgctggct ataagcaccg cgataaaact aaggaagcta acccagccgc cctggccaat
     5281 ctgcaacagt gctccgacaa caataatccc tgaaatcgcg ttggtgaccg acatcaacgg
     5341 tgtatgcagc gcgtgcgata cattccacac cacgtaataa ccgacaacgc aggccagcgc
     5401 gaaaacggtg aagtgcccaa gaaactcttt cggcgcaacg cttgccagcc aaccgaaaag
     5461 gatgattgcc agcgccatca acgcgtattt acgccacggt gagcaggcac atttttcctc
     5521 agttttcact tccggtgccg ctttttgtgc cgcctgcggc tgagctgata cctgaatcgg
     5581 cggtgccggc caggtaattt cgcccgcacg gatcacggtc acgccgcgaa tcaccacatc
     5641 atcaaaatca acagtgatat tgccgtcttt ctctttgcac aacagtttca gcagattaac
     5701 gaggttagta ccgtaaagct gcgaggattg cgtcggcaga cggcccggaa gatcggtata
     5761 accaatcact ttgacaccat tttccgtagt gaagatttca cccggcacgg tgtattcaca
     5821 gttgccaccg ttttgggctg ccaggtcgac aatcacactg cccgccttca tggagtcaac
     5881 catttcacgg gtaattagct tcggcgctgg tttgcctgga ataagcgcgg tggtgacaat
     5941 gatatcgacc tcttttgcct gggcggcaaa gagttccatt tccgctttaa tgaacgcgtc
     6001 cgacatcact ttggcatagc catcgccgct gcccgcttcc tctttaaaat ccagctcgag
     6061 gaattccgcg cccatacttt gaacttgttc tttcacttcc ggacgggtgt cgaatgcacg
     6121 cacaatcgcg ccgagactgt ttgctgcgcc aatggcggcc agacctgcaa cacccgcacc
     6181 aatcaccatc acttttgccg gtggcacttt cccggccgca gtaatttgcc cggtaaagaa
     6241 gcgcccaaat tcatgtgccg cttcaacaat ggcgcgataa ccggcgatgt tcgccatcga
     6301 gcttagtgcg tccagcgatt gtgcgcgtga gatacgcggc acagaatcca tcgccatcac
     6361 ggtcacgtta cgttccgcaa gtttttgcat taattccgga ttctgcgcag gccagataaa
     6421 actcaccagc gttgtcccag gattcagtaa cgcaatttca tcatctaacg gcgcattgac
     6481 cttcagaatg atctctgact gccagacgct attcccttct acaatttcag cgcccgcttg
     6541 cacaaacgct ttatcgtcaa aacttgccag ttgacccgcg ccgctctcta ccgcgacggt
     6601 aaaacccagt ttcagcagct gttccactgt ttttggcgtt gctgcaacac gggtttcatt
     6661 ggttaaccgt tctcttggta tgccaattcg catgatattc ccttccatcg gttttattga
     6721 tgatggtttg cctgtgtcag gagccacaca agccgctcat gtacgagcta aatgttactc
     6781 cgttaaaata aattagtaac aaacgcccta taacgtactg aaaattatgc ctgtgatcta
     6841 gcgccaaaaa tcagtatttc ggcgtgaact cgcaaaatat taacgattca gccgtgatag
     6901 tgggataaac accttagaac gccggataaa gactgataat tgtcttcgac ggtcgggtaa
     6961 aacgagacat cgccccggca cgattcacta cttaacatta aattaactta tacaattcag
     7021 ttgcttcagt agtaatgatg ctgatacggc tgttttttaa gcatagacgg tcatttgagc
     7081 aggattaaaa ttggcttaag gaatgtgata tgaaaaatga cgcagacagt tacaccgttt
     7141 aaatgcaata atcagccacg tttctcgtta ataacaatac cagtacctgg tttgcgcaag
     7201 gcgaaggatt atttttatga agcttaagaa caccctcctg gcgtcggcac tgctttctgc
     7261 tacggcattc tccgttaacg cagcaacaga actgacaccg gagcaagcgg cagcggttaa
     7321 accttttgac cgtgtagtgg ttaccggtcg ttttaatgct attggcgaag cggtgaaagc
     7381 cgtttctcgt cgcgcagata aagaaggtgc cgcctctttt tatgttgtcg acacttctga
     7441 ttttggtaac agcggtaact ggcgtgtggt cgctgacctc tataaagccg atgctgaaaa
     7501 agcagaagaa acaagtaatc gcgtaattaa cggtgttgtc gaactgccga aagatcaggc
     7561 tgttctgatt gaaccgtttg acacggtcac cgtccagggc ttctatcgta gccagccaga
     7621 agtcaatgat gccatcacca aagcggcaaa agcgaaaggt gcctactctt tctacatcgt
     7681 tcgtcaaatc gatgccaacc aaggcggcaa ccagcgtatt actgcattca tctataaaaa
     7741 agatgctaag aaacgtatcg tccagagccc ggatgtgatc ccggcagatt ccgaagcagg
     7801 acgtgcagct ctggctgccg gtggcgaagc cgcgaagaaa gttgagatcc taggtgttgc
     7861 gactaccgca tcaccaagtt ctgaagtcgg tcgtttcttt gaaacccagt catcaaaagg
     7921 cgggcgttac accgtcatgc tcccggatgg cactaaagtc gaagaactga acaaagcgac
     7981 cgcagcgatg atggtcccgt tcgacagcat caaattctct ggcaactacg gcaacatgac
     8041 cgaagtctcc tatcaggttg cgaaacgtgc cgcgaagaaa ggtgctaagt attaccacat
     8101 cacccgccag tggcaggaac gtggtaataa cctgaccgtc agcgcagatc tgtataaata
     8161 actattcagt tactcctata aggcggcttg atgccgcctt tcgcattttt agtcacaaat
     8221 ttccccgcga ccattgcatg cagacacatc tcttcgtaaa atcccgcgct tcagtggcaa
     8281 actccatttt tatgcgtttt gccctaatat ttattcttct tatcacgttt taatcactgg
     8341 atatcgatgg aaaagaaact gggactgagc gcactcaccg cgctggtatt aagctcaatg
     8401 ctgggcgcgg gtgttttcag tctgccgcaa aatatggcgg cagttgccag cccggcagca
     8461 ctgctcatcg gctggggtat tactggcgct ggcattttat tgctggcctt tgccatgctg
     8521 atcctcacgc gcattcgtcc cgaacttgac ggcggtatct ttacctatgc ccgcgaaggg
     8581 tttggcgagc taatcggctt ttgttccgca tggggatact ggctgtgcgc agtcatcgcc
     8641 aacgtctcct atctggtgat cgttttttcc gcgttaagct tttttacgga cacgccggaa
     8701 ttgcgcctgt tcggcgatgg taatacctgg caatcgatcg tcggtgcatc agctttattg
     8761 tggattgttc actttttgat cctgcgcggg gtgcaaaccg ctgccagcat taacctggtg
     8821 gcgacactgg caaaattgtt gccgctgggt ctgtttgttg tgctggcgat gatgatgttc
     8881 aaactggata ccttcaagct cgacttcacc ggacttgcac ttggcgtacc cgtttgggaa
     8941 caggtgaaaa acaccatgct gatcaccctg tgggttttca ttggtgtgga aggtgccgtg
     9001 gttgtttctg cgcgtgcgcg taataaacgt gatgttggca aagcgacact gctggcggtt
     9061 ctctccgctc tgggcgttta cttgctggta acgctgcttt cgctgggtgt ggtggcacgt
     9121 cctgaactgg ctgaaattcg taacccgtca atggccggtc tgatggtgga aatgatggga
     9181 ccatggggcg aaatcatcat cgctgccggt ttgatcgttt ccgtttgcgg cgcgtactta
     9241 agctggacca tcatggcggc agaagttccg ttcctggctg ccactcataa agcattccca
     9301 cgcatttttg cgcgccagaa cgcacaagct gcgccatccg cctcactgtg gttgaccaac
     9361 atctgcgtgc aaatttgtct ggtactcatc tggctgaccg gttccgatta caacacgttg
     9421 ctgaccatcg cctcagaaat gattctggtg ccctatttcc tggtcggcgc gttcttgctg
     9481 aaaatcgcaa cccgtccact gcataaagcg gtaggtgtcg gtgcctgcat ttatggctta
     9541 tggttattgt acgcctctgg tccaatgcac ttgctgttat ccgttgttct gtatgcgccg
     9601 gggctgctgg ttttcctgta tgcgcgcaaa acgcatacac atgataatgt actgaaccgt
     9661 caggaaatgg tgctgattgg catgctgtta attgcctcag ttccggcgac ctggatgctg
     9721 gtgggataac gttcatccca tcgttggata gcaaaggaga taacgatggg taaagcccag
     9781 cccttgccaa tattaattac tggcggaggt cgtcgcatcg gcctcgccct cgcctggcat
     9841 ttcattaatc aaaagcaacc ggtgattgtc agctatcgga cgcactatcc agccattgat
     9901 ggactgatta aggcaggtgc gcagtgtatt caggctgatt tttcgaccaa cgacggtgtg
     9961 atggcgtttg ccgatgaagt actaaaaagc ccccatggtc tgcgtgctat tttgcataac
    10021 gccagtgcgt ggatggcgga aaaaccgggt gcgccactgg ccgacgtact ggcttgcatg
    10081 atgcagatcc acgttaatac cccatacctg ctcaaccatg cgctggaaag attactgcgt
    10141 gggcacggac acgccgccag cgatatcatt cactttaccg attatgtggt ggagcgcggt
    10201 agcgacaagc atattgcgta tgctgcaagc aaagcggcac tggataatat gacccgctcg
    10261 tttgcccgca agctggcacc ggaagtgaaa gtgaactcta ttgctccatc gctgatcctg
    10321 tttaatgaac atgatgatgc cgaatatcga caacaggcgc tgaataaatc actgatgaaa
    10381 accgcgcctg gcgagaaaga agtgatcgac ctggtcgatt acttacttac cagttgcttt
    10441 gtcaccggac gcagtttccc acttgatggc ggtcgtcatc tgcgttaatg cagctttatc
    10501 cagcatataa tcaacaccca tgcgcttatc ccccaacacg aaacagaacc aacaaaagcg
    10561 gcgggcaagc gcatcattcc ggtgaaatac cacagcgaca ccaggtaaac aaaataggga
    10621 ataatcgacc acatactaaa aatgatggtt gcgcgtaagg cttcaatgcc gcgttcgctg
    10681 gcaacaatat aatgcgcgat aagcgcaaag gtcgggaaaa gtggaatcag cccggcgata
    10741 taataatttt tcgtttttgc taaaacacca atcaacagca ctaccagcgc accgagcgcg
    10801 gctttgatta ccagccccat ttttttacct taacacttcc ataacaagtc atcagtagaa
    10861 tacctgatga aaacttgttt agaaacgatt gatagtaagt aaaaacagcg cggtgtattg
    10921 tgacgttttt atatctaccg tgaatgttat gaacactatc gtatttgtgg aagatgatgc
    10981 ggaagtcggt tcactgattg ccgcgtacct ggcaaaacat gatatgcagg ttaccgtaga
    11041 gccgcgcggc gaccaggccg aagaaaccat tttgcgagaa aatccggatt tggtgttact
    11101 cgacatcatg ctaccaggca aggacggcat gaccatttgt cgtgatttac gcgcaaagtg
    11161 gtctggaccg attgttcttc taacctctct cgatagcgat atgaaccaca tcctggcact
    11221 ggaaatgggt gcctgcgact atattctcaa aacgacgccc cctgctgttt tgctagcgcg
    11281 tttacgtttg catttgcgtc agaatgagca agccacactg accaaaggtc ttcaggaaac
    11341 gtctctgact ccctacaaag ccctgcattt cggcacgttg accatcgatc ccatcaaccg
    11401 cgtagtcacc ctggctaaca ctgaaatctc gctctcgaca gctgatttcg aattattgtg
    11461 ggaattagct acccatgccg ggcaaatcat ggaccgcgat gcattgctga aaaatttacg
    11521 cggcgtcagt tatgacggac tggatcgtag cgtggacgtg gctatttcgc ggttaagaaa
    11581 aaaactgctc gataacgccg cagaacctta tcgcattaaa actgtgcgta acaaaggcta
    11641 tctttttgcg cctcatgcat gggaataagc gatgaaaaaa ctgtttatcc agttttacct
    11701 gttattgttt gtctgcttcc ttgtgatgtc tctgctggtt gggctggtgt acaaatttac
    11761 cgccgaacgc gcgggcaaac agtcgctgga tgatttgatg aacagttcac tgtatctgat
    11821 gcgcagcgaa ttgcgtgaga tccccccaca cgactggggt aagacgctga aagagatgga
    11881 tttaaatctc tctttcgatc tgcgtgtcga gccactgagt aaataccatc ttgatgatat
    11941 ttccatgcac cgactgcgtg gcggcgaaat tgtcgccctg gacgatcagt acacgttttt
    12001 gcagcgtatc ccgcgcagcc actacgtgct ggcagttggt cctgttcctt atctttatta
    12061 tctccatcag atgcgattgc tggatatcgc cctgatcact tttattgcta tttccctcgc
    12121 ctttccggtg tttatctgga tgcgtccgca ctggcaggat atgttaaaac tggaagcagc
    12181 ggcgcaacga tttggcgatg ggcatctcaa tgaacgtatc cactttgatg agggttcgag
    12241 ctttgaacga cttggtatcg catttaacca gatggcggac aatatcaacg ccttgattgc
    12301 cagcaaaaaa cagcttattg acggtatcgc tcacgaactg cgaacaccgt tagtgcgcct
    12361 gcgttatcga ctggagatga gcgataacct gagcgccgcc gaatcccagg cgttgaatcg
    12421 tgatatcagt caacttgaag ctttaattga agagctgctg acttatgccc gactcgatcg
    12481 cccacaaaac gagcttcatc ttagcgaacc agacctgccg ttgtggctgt caacgcatct
    12541 ggcagacatt caggcagtaa cgcccgataa aacggtacgg attaaaacgc tcgcgcaagg
    12601 ccattatgcg gcgttggata tgcgcttaat ggagcgcgtg ctggataatt tgctcaataa
    12661 cgccctgcgg tactgccatt caacggttga aaccagcctg ctactgtcgg ggaatagagc
    12721 gacattaatt gttgaggatg atggcccagg gattgcccca gaaaaccgcg aacatatctt
    12781 tgaacctttt gttcgcctcg atcccagccg ggatcgctca accggcggct gcgggctggg
    12841 gctggcaatt gtccactcta tagcactggc aatgggcggt acggttaatt gtgacaccag
    12901 cgaactgggt ggtgctcgct tctcgtttag ctggccgtta tggcataaca tcccgcaatt
    12961 tacctctgcc tgacactacg cgcacgatgg tcaagtcacc accactgtgc tataaaataa
    13021 gtatgttgta actaaagtgg ttaatattat ggcgcgttac gatctcgtag accgactcaa
    13081 cactaccttt cgccagatgg aacaagagct ggctgcattt gccgctcatc ttgagcaaca
    13141 caagctattg gttgcccgcg tgttctcttt gccggaggta aaaaaagagg atgagcataa
    13201 tccgcttaat cgtattgagg taaaacaaca tctcggcaac gacgcgcagt cgctggcgtt
    13261 gcgtcatttc cgccatttat ttattcaaca acagtccgaa aatcgcagca gcaaggccgc
    13321 tgtccgtctg cctggcgtgt tgtgttacca ggtcgataac ctttcgcaag cagcgttggt
    13381 cagtcatatt cagcacatca ataaactcaa gaccacgttc gagcatatcg tcacggttga
    13441 atcagaactc cccaccgcgg cacgttttga atgggtgcat cgtcatttgc cggggctgat
    13501 cacccttaat gcttaccgca cgctcaccgt tctgcacgac cccgccactt tacgctttgg
    13561 ttgggctaat aaacatatca ttaagaattt gcatcgtgat gaagtcctgg cacagctgga
    13621 aaaaagcctg aaatcaccac gcagtgtcgc accgtggacg cgcgaggagt ggcaaagaaa
    13681 actggagcga gagtatcagg atatcgctgc cctgccacag aacgcgaagt taaaaatcaa
    13741 acgtccggtg aaggtgcagc cgattgcccg cgtctggtac aaaggagatc aaaaacaagt
    13801 ccaacacgcc tgccctacac cgctgattgc actgattaat cgggataatg gcgcgggcgt
    13861 gccggacgtt ggtgagttgt taaattacga tgccgacaat gtgcagcacc gttataaacc
    13921 acaggcgcag ccgcttcgtt tgatcattcc acggctgcac ctgtatgttg cggattaacg
    13981 cccggctttc atactgccga ccatctgttc tggccgtacc cagctgtcaa actcggcttc
    14041 gctaagatac cccagcgcaa gggccgcagc ttttaaggtc agcccttctt tatgcgcttt
    14101 tttggcgatc tccgcggctt tgtcataacc aatgtgggtg ttaagcgcag tcaccagcat
    14161 cagcgattca ttgagtaatt gattgattcg ctcacgattc ggttcaatac ccactgcgca
    14221 gtgtttgtta aaactttcca tgccatctgc cagcaagcgc accgattgca ggaaattgtg
    14281 gatcaccatt ggacggaaga cgttcagttc aaagttaccg gaagcgccac ccatgttgat
    14341 cgccacgtcg ttccccatta cctgacagca gagcatggtt aatgcttcac actgggttgg
    14401 gttcactttc cccggcatga ttgagctgcc cggctcattt tccgggattg agatttcacc
    14461 aattccgcag cgcgggccag aggccagcca gcggacatca ttggcgattt tcatcagtga
    14521 cgcagccaac cctttcaacg cgccgtgcgc ctgaaccagg gcatcacagg tcgcaagcgc
    14581 ttcaaatttg ttcggcgcgg taacaaacgg tgcacaggta atgactgcca gttcatctgc
    14641 tacgcgacgc gcatactccg gatgggtatt tagtccagta cccaccgctg taccgccgag
    14701 agccagttcc gctacgtgag gcaggctgta ttcgatatgt ttgagattat gctcgagcat
    14761 cgctacccag ccggaaatct cctgccccag tgttagcggc gtggcgtcct gcaagtgggt
    14821 tcgaccgatt ttgacgatat cggcaaatgc acgcgatttt tcactcagtg tctgtgtcag
    14881 ggttttaacc tgcggaatga gttgcttgcg cagcgccagc agcgccgcaa cgtgcatcgc
    14941 cgtcggaaag acatcgttgg aactttggct tttgttcacg tcgtcgttag ggtgaacttt
    15001 acgttccatc ccgcgcacgc cgccgagtaa ttcactggcc cggttagcca gcacttcgtt
    15061 catgttcatg ttactttgcg tgccggagcc ggtctgccag atagccagcg ggaattcgtc
    15121 gtcatgctgt cctgccagta cttcatccgc cgcctgccga atggcgctcg ctttctcttc
    15181 agacaacaag cctaaatctt cattaacttt tgccgctgcg cgcttggtta gcgccagcgc
    15241 atgaatcagt gaggtgggca ttttctccgt cgaaatgcgg aaatgctcca gcgagcgttg
    15301 agtttgtgcg ccccacagct tatctgccgg gacatcaatc gcccccatcg aatctttttc
    15361 gctgcgtact gtattcatgg cctgctcctc acctgattaa ttttttcttt ctgttttgcc
    15421 ttcgttaagc aacttttagc tcacttatta tttactattt gataacaaat gtttggtctt
    15481 tcgtgccatg taaaaaaacc gccccgaagg g
//
LOCUS       DAESHI010000099        15406 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.100, whole genome shotgun sequence.
ACCESSION   DAESHI010000099 DAESHI010000000
VERSION     DAESHI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.100"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            10..453
                     /locus_tag="KW011_003176"
     CDS             10..453
                     /locus_tag="KW011_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH7661447.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGGDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            669..758
                     /locus_tag="KW011_003177"
                     /pseudo
     CDS             669..758
                     /locus_tag="KW011_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(759..1817)
                     /gene="modC"
                     /locus_tag="KW011_003178"
     CDS             complement(759..1817)
                     /gene="modC"
                     /locus_tag="KW011_003178"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HBH7661448.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1820..2509)
                     /gene="modB"
                     /locus_tag="KW011_003179"
     CDS             complement(1820..2509)
                     /gene="modB"
                     /locus_tag="KW011_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBH7661449.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2509..3282)
                     /gene="modA"
                     /locus_tag="KW011_003180"
     CDS             complement(2509..3282)
                     /gene="modA"
                     /locus_tag="KW011_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH7661450.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3448..3597)
                     /gene="acrZ"
                     /locus_tag="KW011_003181"
     CDS             complement(3448..3597)
                     /gene="acrZ"
                     /locus_tag="KW011_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HBH7661451.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3726..4514
                     /gene="modE"
                     /locus_tag="KW011_003182"
     CDS             3726..4514
                     /gene="modE"
                     /locus_tag="KW011_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HBH7661452.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4582..6054
                     /gene="modF"
                     /locus_tag="KW011_003183"
     CDS             4582..6054
                     /gene="modF"
                     /locus_tag="KW011_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HBH7661453.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6315..7331
                     /gene="galE"
                     /locus_tag="KW011_003184"
     CDS             6315..7331
                     /gene="galE"
                     /locus_tag="KW011_003184"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBH7661454.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7341..8387
                     /gene="galT"
                     /locus_tag="KW011_003185"
     CDS             7341..8387
                     /gene="galT"
                     /locus_tag="KW011_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBH7661455.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8391..9539
                     /gene="galK"
                     /locus_tag="KW011_003186"
     CDS             8391..9539
                     /gene="galK"
                     /locus_tag="KW011_003186"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBH7661456.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9533..10573
                     /gene="galM"
                     /locus_tag="KW011_003187"
     CDS             9533..10573
                     /gene="galM"
                     /locus_tag="KW011_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HBH7661457.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10776..11528
                     /gene="gpmA"
                     /locus_tag="KW011_003188"
     CDS             10776..11528
                     /gene="gpmA"
                     /locus_tag="KW011_003188"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBH7661458.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11687..12739)
                     /gene="aroG"
                     /locus_tag="KW011_003189"
     CDS             complement(11687..12739)
                     /gene="aroG"
                     /locus_tag="KW011_003189"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HBH7661459.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13055..13435
                     /locus_tag="KW011_003190"
     CDS             13055..13435
                     /locus_tag="KW011_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HBH7661460.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            13549..14490
                     /gene="zitB"
                     /locus_tag="KW011_003191"
     CDS             13549..14490
                     /gene="zitB"
                     /locus_tag="KW011_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HBH7661461.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14487..15206)
                     /gene="pnuC"
                     /locus_tag="KW011_003192"
     CDS             complement(14487..15206)
                     /gene="pnuC"
                     /locus_tag="KW011_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HBH7661462.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15244..>15406)
                     /gene="nadA"
                     /locus_tag="KW011_003193"
     CDS             complement(15244..>15406)
                     /gene="nadA"
                     /locus_tag="KW011_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBH7661463.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         3917 a         4042 c         3851 g         3596 t
ORIGIN      
        1 ggcctttgca tggatcttgc actggtaaca ggacagcgcg aaggagatct ggcggctatg
       61 agatgggagg atataagaga tggcaggcta tacgtggaac aacaaaaaac aggggcaaaa
      121 attcgcattt ctctccccac cacgatttcc agattgaatt taacacttgc cgatgtactg
      181 gataacttga aaaaaataaa cggtaaaaac gaaaagttac tcggagggaa gacagccagg
      241 acaatcgcag cacagttcag gattgcccga gacacatccg gattgaaatg ggaaggtgac
      301 ccaccgccat ttcatgaaat ccgaagttta tccggtcgcc tgcactcagc agaaaaaggc
      361 ggtgatttca ctcaggcgct ccttggacat cgttcatcca gcatgacgga taaataccgt
      421 gacggacgag ggagggaatg gaaagacatt tagaattaat ttcgaatgat ttcgaaacac
      481 cacttgtaat taattgatta taaatgatta attcctaatg ggcatttgct tcatgaatgc
      541 tcctttgcat ttagcaactg actgtaacag ctaaaattag tcgcttttgg cggtgaatgc
      601 tgaattaaaa cgcagaattt tgtccgatag tggtatcggt gtaccattcg cgcagacgaa
      661 aattcttaaa tgacgcggta aaagcgcgcg ccaacattgt gattggtgat aacaccaccg
      721 acagcattgc ccagttcatt tatagccacc tgatttaatc aggcggttat cgacacactt
      781 ttaatttgcg cgtacagcca caggccaggt ttgatcgcca gttcatccct ggcccacggg
      841 ctgatacgcg cccacagcgt tttaccgccg acttccagct ccacttccac ctggccgttg
      901 tcgtcataac tattaacaac ttttgcccgc aatacgttac gaatgctggt ttgctgcggc
      961 ggttgtaaaa ccagagaaac atcggaagcc tgaatgcgga tacgtagcgc agcttgcagc
     1021 ggttcgtcca gcttattgac ccacaaatgc tgatcgccca gcgccagcgc ggtcatcgcg
     1081 taatgcggat gatgctcaag taccgtcact ttcagaatgc tactttgttg ctctttcggc
     1141 agccacggat tcatcacgct actgccccac acttcctcca gcgcgccaaa ggctttcacc
     1201 tgaccgtttt ccagtaccat cactctgtct gccagatgga ggatctcatc cagcgaatgg
     1261 ctgacataca acatcggaat gttgatttcc cgtgtcagcc gttgcagata aggcaacagt
     1321 tcgcgtttac gcggaatatc cagtgacgcc agcggttcat ccagcaacag caattccggt
     1381 gctgtcagca aagcccgacc aatcgccacg cgctgttttt cgcctccgga caggctgcct
     1441 ggtaaacggt caagcaacgg ttcaatgcct aaaagcgcca ccagcttatc gaactgatcg
     1501 accatacttt tcgacatgcc gtagcgcaga ttgccacgca ctttgtaatg cgggaacagc
     1561 cgcgcatcct gaaaaacata gccaacgcga cgcttttccg gcgtcaggca gatacctttt
     1621 tcggcatcat ttagtacccg cccattgagg acaatccgcc ctttttgcgg gcgcgtcagt
     1681 ccactgatgg cgttaatcag cgaagttttt ccggcaccgg agacgccaaa gatagcagtg
     1741 atgccattgg cgggcagtgt ttcattaatg gtcaggcaat ggttgcccaa cgtctgggaa
     1801 aaattcagct ccagcatgat tagcgccccg cccgttcacg gctgattctg gccagccatt
     1861 ctgaaatcaa cagggagatc atcgccagcg caatagaaat aatgcacagt ctcgccgcgc
     1921 cactttcccc gccgggggtc tggatcaggg tatacatggc agagggaatg gttcgcgttt
     1981 cgccaggaat gttcgacaca aaggtgatgg ttgcaccaaa ctcaccgaga gaacgagcaa
     2041 aagccagtac cgtaccaaca ataattcccg gtaaggtcag cggtaacgtg atagtaaaga
     2101 aaacgcgcca gcgcccggca cctagtgttc ttgcggcctg ttcgagtttg acatcaaccc
     2161 cttccagcgc cagacgaatt gcccgcacca tcagcggaaa cgacatgacg gcggcagcga
     2221 gaaccgcgcc gcgccagcta aaggcgaagg taataccaaa ccagtcatac agacgttcac
     2281 cgataaatcc gcgccgtccc atcgaaacta ataataagta accgacgacc acgggcggta
     2341 acaccagcgg tagatgcagt acgctgtcga gcagagcttt gcccggaaac gtgcaacgca
     2401 ccagtaacca ggcaaaaaag atcccaaacg gcaggctaaa cagcacagcc agggaagaaa
     2461 ctttcaggct taataaaact gcctgccatt ctggatcggt cagtatcatt acttggttgt
     2521 aaatccgtaa cgtttaaaga tttccgctgc ctgcggtccc ttcagataat cataaaatgc
     2581 tttcactgtc gcattgttat gcccttccac aacagcaacc ggatattcca cttttttatg
     2641 tgaatcttcc gggaaggtgg caaccacttt tacccctttg ctgacaactg cgtcagaacc
     2701 gtaaacaatg cccaacggcg cttcattgcg ttcgaccaac gccagcgcgc cacgaacatc
     2761 ttccgccgga gccagtttcg gggagagcgt atcccatgcg cccagttttt gcagtgcttc
     2821 ttttgcataa atgccagcag ggacatgttc cggatcgcca accgcgaggc gaccgccatt
     2881 cagcagcgaa gtccagttag ttgtgctgtc gatgatgaaa tctttctgct cgctggcttt
     2941 cggtgctaca acgaccaggc tattgccgag cagtgtctgg cgcgaagccg tatcgatcgc
     3001 ttttttatca accgcataat ccatccattt ctgatcggca gagataaaca gatccgcagg
     3061 cgctcccgct tcaatctgac gggccagagt agatgacgaa gcgaaagaag aaaccacatc
     3121 cacgcctttt tcttttttat actgcgtagc aatgtcctgc attgcgttag tcagtgatgc
     3181 ggcggcgaac accgtgattt tcccttcatc tgccagtgta ttgccagcaa cagcgaaaga
     3241 gagtgccgcc ccggcaaaca agttcaacca tttacgagcc attggtaacc ccttaatgta
     3301 acgttatata ggtgacaata taacggctag ctcagaattt tcccagcagt tattgctaac
     3361 ctttgtgagg tagataagaa aaaatatcgg ctggaaaagc aggaagttga gaaaaagaaa
     3421 tgcccgacta agcgggcatt cagggaatca atgattttgt ccgggctggt cttttttacc
     3481 aacaccagaa aagatgttga atacttcacc aagaccgtaa atcagaccca ggatgatggc
     3541 catcacgaca ggtaccatga ttacggcgaa taccagactt tttaataact ctaacatggt
     3601 caactccaga tatagtcatg agactattct aaccgctaag cacagaaaag cactcccctt
     3661 ttgtgcggtc agctttgcgt ggcgttcgtt ttccgtcaca ataagacttt tgccaggaca
     3721 ttgttatgca ggccgaaatc cttctcaccc ttaagctcca acaaaaatta ttcgccgacc
     3781 cgcgccggat ttcgctacta aaacacattg cgctttccgg ttccattagc cagggagcga
     3841 aagatgccgg tatcagctat aaaagcgcct gggatgccat taacgagatg aatcagttaa
     3901 gcgagcatat tctggttgag cgcgcaacag gcggtaaagg tggcggcggc gcagtactga
     3961 cccgctatgg tcagcgactg attcagctct atgacttact ggcgcaaatc cagcaaaaag
     4021 cctttgatgt gttaagtgac gatgacgccc tgccgctgaa cagcctgctg gccgcgatct
     4081 cacgtttttc actgcaaacc agcgcccgta accagtggtt cggtaccatc accgcccgcg
     4141 atcatgatga cattcaacag catgttgatg tcttactggc tgacggaaaa acacgcctga
     4201 aagtcgcaat taccgcacaa agcggcgcac gtctggggct ggatgaaggc aaagaagtgt
     4261 tgatattgct aaaagcgccg tgggtaggca ttactcagga cgaagcggtc gcacaaaacg
     4321 ccgacaacca attaccgggt attattagtc atattgagcg cggcgcagag cagtgcgaag
     4381 tattaatggc gctacccgac gggcaaacac tgtgcgccac agtgccggta aatgaagcga
     4441 cttctcttca gcaagggcag aatgtcacgg cctactttaa tgccgacagc gtgattatcg
     4501 ccacgctgtg ctaagcgtgt tgacaatttg ttatgaaaca cgtatccctg tcagtaatcg
     4561 ctgcacaaaa tgggatataa aatgtcatcg ttgcaaattt tgcaaggcac gtttcgtctt
     4621 agcgacacaa aaacgctgca attgcctcag ctaacgttaa acgcgggtga tagttgggcg
     4681 tttgtcggtt cgaatggaag cgggaaatcg gcactggccc gcgcgctggc gggggaactt
     4741 ccgcttttga aaggtgaacg gcaaagccag ttttcccaca tcactcgtct ctccttcgag
     4801 caattgcaaa aactcgtcag cgacgaatgg cagcggaata acaccgatat gctcggccct
     4861 ggcgaagatg acaccggacg cactacggct gaaatcattc aggatgaagt aaaggatgca
     4921 ccgcgttgca tgcaactggc gcagcagttc ggtattaccg ccctcctcga ccgacgcttt
     4981 aaataccttt ccactggcga gacgcgaaaa accctgctgt gtcaggcgct gatgtcggag
     5041 cctgacttgt tgattcttga tgagtcgttc gatggcctgg atgttgcctc acgtcagcag
     5101 ctggctgagc gactcgcctc gttacatcag tccggtatta ctctggtact ggtgctcaat
     5161 cgcttcgatg agatcccgga gtttgtccag tttgctggcg tgctggcgga ttgcacgtta
     5221 gcggaaactg gcgctaaaga ggaactgctc caacaagcac tcgtcgcgca actggcgcat
     5281 agtgaacagc ttgaaggtgt gcaactgccg gagccggatg aaccttcagc acgtcacgcc
     5341 ttacccgcca acgaaccgcg cattgtgctg aacaatggcg tggtttctta taacgatcgc
     5401 cccattctta ataaccttag ctggcaggtg aatccaggtg aacactggca aattgtcggg
     5461 ccaaatggtg caggaaaatc gacgttatta agcctggtta ctggcgatca tccgcaaggt
     5521 tacagcaacg atttgacgct tttcggacga cgtcgcggca gcggcgaaac catctgggat
     5581 atcaaaaagc atatcggtta cgtcagcagt agtttgcatc tggattaccg ggtcagcact
     5641 accgtgcgta atgtgattct ttctggctat tttgattcga ttggcattta tcaggccgtt
     5701 tcggatcgcc agcaaaaact ggtgcagcag tggctggata ttctcggcat tgataaacgt
     5761 acggctgacg ctccgttcca tagtctttcc tggggacagc agcgtctggc gctgatcgtc
     5821 cgcgcactgg tgaaacatcc gacgttgctt attctcgatg aaccactaca ggggcttgat
     5881 ccgctcaatc gccagcttat ccgccgtttt gttgatgtgc tgattagcga aggtgaaacg
     5941 caattgttat ttgtttcgca ccacgctgaa gatgcgcctg cctgtattac ccatcgcctt
     6001 gagttcgtgc cggacggtgg actctatcgc tatgtgctga caaaaatata ttgagtcggt
     6061 agtgctgacc ttgccggagg cggcctaagc gccctctccg gccaacggtt cgacgcatgc
     6121 aggcatgaaa ccgcttcttt tttcagataa aaagcgcaat cattcataaa ccctctgttt
     6181 tataatcact taatcgcgca taaaaaacgg ctaaattctt gtgtaaacga ttccactaat
     6241 ttattccatg tcacactttt cgcatctttg ttatgctatg gttatttcat accataagcc
     6301 taatggagcg aattatgaga gttctggtta ccggtggtag cggttacatt ggaagtcata
     6361 cctgcgtgca attactgcaa aacggtcatg atgtcatcat tcttgataac ctctgtaaca
     6421 gtaagcgcag cgtactgcct gttatcgagc gcttaggcgg caaacatcca acgtttgttg
     6481 aaggcgatat tcgtaacgaa gcgttgatga ccgagatact gcacgatcac gctatcgaca
     6541 ccgtgatcca cttcgccggg ctgaaagccg tcggcgaatc ggtacaaaaa ccgctggaat
     6601 attacgacaa caatgtcaac ggcactctgc gcctgattag cgccatgcgc gccgctaacg
     6661 tcaaaaactt tatttttagc tcctccgcca ccgtttatgg cgatcagccc aaaattccat
     6721 acgttgaaag cttcccgacc ggcacaccgc aaagccctta tggcaaaagc aaattgatgg
     6781 tggaacagat cctcaccgac ctgcaaaaag cccagccgga ctggagcatt gccctgctgc
     6841 gctacttcaa cccggttggc gcgcatccgt cgggcgatat gggcgaagat ccgcaaggca
     6901 ttccgaataa cctgatgcca tacatcgccc aggttgctgt aggccgtcgc gactcgctgg
     6961 cgatttttgg taacgattat ccgaccgaag atggtactgg cgtacgcgat tacatccacg
     7021 taatggatct ggcggacggt cacgtcgtgg cgatggaaaa actggcgaac aagccaggcg
     7081 tacacatcta caacctcggt gctggcgtag gcagcagcgt gctggacgtg gttaatgcct
     7141 tcagcaaagc ctgcggcaaa ccggttaact atcattttgc accgcgtcgc gaaggcgacc
     7201 ttccggccta ctgggcggac gccagcaaag ccgaccgtga actgaactgg cgcgtaacgc
     7261 gcacactcga tgaaatggcg caggacacct ggcactggca gtcacgccat ccacagggat
     7321 atcccgatta aggaacgacc atgacgcaat ttaatcccgt tgatcatcca catcgccgct
     7381 acaacccgct caccgggcaa tggattctgg tttcaccgca ccgcgctaag cgcccctggc
     7441 agggggcgca ggaaacgcca gccaaacagg tgttacctgc gcacgatcca gattgcttcc
     7501 tctgcgcagg taatgtgcgg gtgacaggcg ataaaaaccc cgattacacc gggacttacg
     7561 ttttcactaa tgactttgcg gccttgatgt ctgacacgcc agatgcgcca gaaagcaacg
     7621 atcctttgat gcgttgccag agcgcgcgcg gtaccagccg ggtgatctgc ttttcaccgg
     7681 atcacagtaa aacgttgcca gaactgagcg ttgcggcatt gacggaaatc gtcaaaacct
     7741 ggcaggagca aaccgcagag ctgggaaaaa cgtacccgtg ggtgcaggtc tttgaaaaca
     7801 aaggtgcggc gatgggctgc tctaacccgc atccgcacgg acagatttgg gcaaatagct
     7861 tcctgcctaa cgaagctgag cgcgaagacc gcctgcaaaa agaatatttt gccgaacaga
     7921 aatcaccaat gctggtggat tatgttcagc gcgagctggc agacggtagc cgtaccgttg
     7981 tcgaaaccga acactggtta gccgttgtgc cttactgggc tgcctggccg ttcgaaacgc
     8041 tactgctgcc caaagcccac gttttgcgga tcaccgattt gaccgacgcc cagcgcagcg
     8101 atttggcgct ggcgttgaaa aagctgacca gtcgttatga caacctcttc cagtgctcct
     8161 tcccctactc tatgggctgg cacggcgcac catttaatgg cgaagagaat caacactggc
     8221 agctgcacgc gcacttttat ccgcctctgc tgcgctccgc caccgtacgt aaatttatgg
     8281 ttggttatga aatgctggca gagacccagc gagacctgac cgcagaacag gcagcagagc
     8341 gtttgcgcgc agtcagcgat atccattttc gcgaatccgg agtgtaagaa atgagtctga
     8401 aagaaaaaac acaatctctg tttgccaacg catttggcta ccctgccact cacaccattc
     8461 aggcgcctgg ccgcgtgaat ttgattggtg aacacaccga ctacaacgac ggtttcgttc
     8521 tgccctgcgc gattgattat caaaccgtga tcagctgtgc accacgcgat gaccgtaaag
     8581 ttcgcgtgat ggcagccgat tatgaaaatc agctcgacga gttttccctc aatgcgccca
     8641 ttgtcgcgca tgaaaactat caatgggcga actacgttcg tggcgtggtg aaacatctgc
     8701 aactgcgtaa caacagcttc ggcggtgtgg acatggtgat cagcggcaat gtgccgcagg
     8761 gtgccgggtt aagttcttcc gcttcactgg aagtcgcggt cggaaccgta ttgcagcagc
     8821 tttatcatct gccgctggac ggcgcacaaa tcgcgcttaa cggtcaggaa gcagaaaacc
     8881 agtttgttgg ctgtaactgc gggatcatgg atcagctaat ttccgcactc ggcaagaaag
     8941 atcatgcctt gctgattgac tgccgctcac tggggactaa agcagtttcc atgccgaaag
     9001 gtgtggctgt cgtcatcatc aacagtaact tcaaacgtac cctggttggc agcgaataca
     9061 acacccgtcg tgaacagtgc gaaaccggtg cgcgtttctt ccagcagcca gctctgcgcg
     9121 atgtcaccat tgaagagttc aacgctgttg cacatgagct ggacccaatc gtggcgaaac
     9181 gcgtgcgtca tatcctgact gaaaacgccc gcaccgttga agctgccagc gcgctggagc
     9241 aaggcgatct gaaacgtatg ggcgagttga tggcggagtc tcatgcctct atgcgcgatg
     9301 atttcgaaat caccgtgccg caaattgaca ctctggtaga aatcgtcaaa gcagtgattg
     9361 gcgacaaagg tggcgtacgc atgaccggcg gcggatttgg cggctgtatc gtcgcgctga
     9421 tcccggaaga gctggtgcct gccgtacagc aagctgtcgc tgaacaatat gaagcaaaaa
     9481 caggtattaa agagactttt tacgtttgta aaccatcaca aggagcagga cagtgctgaa
     9541 cgaaactccc gcactggcac ccgatggtca gccgtaccga ctgttaactt tgcgtaacaa
     9601 cgcagggatg gtagtcacgc tgatggactg gggtgcgact ttactttcag ctcgtattcc
     9661 gctttccgat ggcagcgtcc gcgaggcgct gctcggctgt gccagcccgg aatgctatca
     9721 ggatcaggcc gcgtttctgg gggcctctat tggtcgttat gccaaccgca tcgccaatag
     9781 ccgttatacc tttgacggtg aaacggtgac gctttcgcca agtcagggcg ttaaccagct
     9841 gcacggcggc ccggaagggt tcgacaaacg tcgctggcag attgtgaacc agaacgatcg
     9901 tcaggtgctg tttgccctga gttcagatga tggcgatcag ggcttcccgg gtaatctcgg
     9961 cgcgacggtg caatatcgtc tgaccgacga taaccgtatc tccattactt atcgcgccac
    10021 agttgataaa tcttgcccgg tgaatatgac taatcacgtc tatttcaatc ttgacggcga
    10081 gcagtctgac gtgcgcaatc acaagttgca gattctggcg ggcgaatatc tgccggttga
    10141 tgaaggcggc attccgcacg acggcctgaa atctgtcgcc ggaacctctt ttgatttccg
    10201 cagcgccaaa atcatcgcca gtgagtttct tgccgacgac gatcagcgca aagtgaaagg
    10261 ttacgatcac gcattcttgt tacaggccaa aggcgatggc aagaaagtgg cggcgcatgt
    10321 ctggtcagca gatgaaaaat tgcagctgaa ggtctacacc accgctcccg ctcttcaatt
    10381 ctactccggc aacttcctcg gcggcacacc gtcgcgggga accgaacctt acgccgactg
    10441 gcaaggactg gcgttggaaa gcgagtttct gccggacagc ccgaaccacc ctgaatggcc
    10501 gcaaccggac tgcttcctgc gtcctggcga agagtattcc agcctgacgg aatatcagtt
    10561 tattgctgag taatgttaag ccctcgtcat gagggcttta tcttcatatt gttcaaatca
    10621 ccagcaaaca ccgacatatt tgcaactcaa tattcacaac aaccttacac tgcgccacta
    10681 ttttcgctat ggttatgcgt aagcattgct gttgcttcgt cacggcaata taatgagaat
    10741 tattatcata aaaagataat ttgaggagta agtatatggc tgtaactaag ctggttctgg
    10801 ttcgtcatgg cgaaagtcag tggaacaaag aaaaccgttt caccggttgg tacgacgtgg
    10861 atctgtctga gaaaggcgta agcgaagcaa aagcagcagg taagctgctg aaagaggaag
    10921 gttacagctt tgactttgct tacacttctg tgctgaaacg cgctatccat accctgtgga
    10981 atgtgctgga cgaactggat caggcatggc tgcccgttga gaaatcctgg aaactgaacg
    11041 aacgtcacta cggtgcgttg cagggtctga acaaagcgga aaccgccgaa aagtatggcg
    11101 acgagcaggt gaaacaatgg cgtcgtggtt ttgccgtgac tccgccggaa ctgactaaag
    11161 atgatgagcg ttatccgggt cacgatccgc gttacgcgaa actgagcgag aaagagctgc
    11221 cgctgactga aagcctggcg ctgaccattg accgcgtgat cccttactgg aatgaaacta
    11281 ttctaccgcg tatgaagagc ggtgagcgcg tgatcatcgc tgcacacggt aactctttac
    11341 gtgcgctggt gaaatatctt gataacatga gtgaagaaga gattcttgag ctgaatatcc
    11401 cgaccggcgt gccgctggtg tatgagttcg acgagaattt caaaccgctg aaacgctatt
    11461 atctgggtaa tgctgacgag atcgcagcga aagcagcggc ggttgctaac cagggtaaag
    11521 cgaagtaaac gtcattcgtt taaaaatgag aaagccgact gcaagtgagt cggctttttt
    11581 gttgctaaca atggagcaca ttgcctgatg cgacgctgcg cgtcttatca ggcctgtggt
    11641 gattcatcgg atacgccact ctgacggcgc atccgacaat taaaccttac ccgcgacgcg
    11701 cttttactgc attcgccagt tgacgtaaca gagcatcggt atcttcccag ccaatgcagg
    11761 catcggtgat gctcttaccg taggccagcg gctccccgct atcgaggctc tgattgcctt
    11821 ccaccagatg gctttccacc atcacgccaa taatggcctt ttcgccaccg gcaatctgct
    11881 ggcaaacgtc agcacaaaca tccatctgct ttttgaattg tttggacgag ttagcatggc
    11941 tgaaatcgat catcacctgt gctggcaggc ctgctttgtt cagcccttct ttcacttcag
    12001 caacgtgctt cgcgctgtag ttaggctctt taccgccgcg cagaatgata tggcaatcac
    12061 cgttaccgct ggtattcaca atcgccgaat gcccccattt agttacggac aggaagcagt
    12121 gcggcgcacc ggcggcatta atggcatcga tagccacttt aatcgtaccg tctgtgccat
    12181 ttttaaaacc taccggacaa gaaagaccag acgccagttc gcggtgcacc tgcgattccg
    12241 tggtacgtgc accaattgcg ccccagctca tcaggtcagc gagatactga ggagtgatca
    12301 tatcgagaaa ctcacccgcc gctggcagac cgctgtcgtt aatatcaagc agcaatttac
    12361 gggctatacg cagaccgtcg ttgatctgga agctgttatc catatgcgga tcgttaatca
    12421 accctttcca gcccaccgtg gtacgcggct tttcaaaata gacgcgcatt acgatttcca
    12481 gctcatcttt cagctcttca cgcagcgcca gcaagcgagt ggcatactct ttagccgcga
    12541 caggatcatg aattgagcac gggccaatca ccaccaacag gcgatcatca ttacctttca
    12601 ggatcttatg gatcgctttt cgggcatggg caaccgtatt cgcggcattt tcagttacgg
    12661 ggaatttttc cagcaatgcg acaggaggaa gtaactcttt gatttctttg atgcgtaaat
    12721 cgtcgttctg ataattcatg tctgttccag tgttgccata cttatcttag tgaatgcaat
    12781 acttccaatc tatatcttcc gtcagaatgt gtaaacgggg ttttacacta tgaacggatt
    12841 aatcctggaa ttagcaagaa aaacgccaga ttgtcgcgaa aaacgagatc tctcctacaa
    12901 tttctaactg taactccttt gtttgttaat tatttcaaga ttctctgctg cgtttcataa
    12961 actggctgaa atcttaaacc aatgccttat attcacctgc aaatgcactg ttggaagagg
    13021 ttatccgaca taacgaccat aacaggagca tcctatgaaa atgacaaaac tggccacatt
    13081 atttctgact gccactctaa gccttgccag cggtgccgca ctggctgccg atagcggagc
    13141 gcaaactaat aacggccagg caaacgccgc agctgatgcg ggccaggtag cccctgacgc
    13201 ccgtgaaaat gtcgcgccaa ataacgtcga caataacggg gtaaataccg gttctggcgg
    13261 cacaatgctg cattcggatg gttcttcaat gaacaatgac ggaatgacca aagatgaaga
    13321 gcacaagaac accatgtgca aagatggtcg atgtccggat atcaataaaa aggtacaaat
    13381 cggtgatggc atcaataatg atgtcgatac caaaaccgac ggtaccacgc agtaaccctg
    13441 caaaatagcc aataatggga gagcggagtc tctccctttt aatttttcac ttctaaaagc
    13501 agtatgatgt cataaagtta agtgagaata attaagggat gactcgtcat ggcgcactca
    13561 cactcacaca cgtcctcaca cctgccagaa gataataatg ctcgtcgctt gttgtatgct
    13621 ttcggcgtga ctgccgggtt tatgctggta gaagtcgttg gtggttttct ttctggttct
    13681 ctggcattgc tggccgatgc gggtcatatg ttgaccgata ctgccgccct gcttttcgcc
    13741 ctactcgccg tgcaattttc acgtcgtcct cccaccattc gccacacttt cggttggcta
    13801 agactgacca cgctcgcggc gtttgtgaat gccatcgccc tcgtggtaat taccattttg
    13861 attgtctggg aggcgataga acgcttccgc acgccgcgtc cggtcgaggg cggcatgatg
    13921 atggcaattg ccgtggctgg gttgctggca aatatacttt ctttctggtt acttcatcac
    13981 ggcagcgaag agaaaaacct caacgtacga gcagcggcat tgcatgtgct cggggacctg
    14041 ttgggttcgg ttggggctat tattgccgcg ttgattatta tctggaccgg ctggacacct
    14101 gctgacccca ttctctcgat agtggtgtcg cttctggttc tgcgtagcgc gtggcgactg
    14161 ttgaaagata gcgtgaatga attacttgaa ggtgcaccgg tatcgctgga tatcgctgaa
    14221 ctgaagcgtc gtatgtgccg ggaaatcccg gaagtccgca atgtgcacca tgtacatgta
    14281 tggatggtag gcgagaagcc ggtgataacg ctgcatgtgc aggtgatccc gccacacgat
    14341 cacgatgcct tgttggatca gatccaacac tacctgatgg atcactatca gattgagcac
    14401 gccaccattc agatggaata tcaaccttgt catgggccag actgccacct gaatgaaggg
    14461 gtgtctggcc attcacatca tcaccattaa tgggacagcg cgcgtgagcc tctttcacgt
    14521 gcgctgttga tccacatccg actgccgttg agcgcaataa aggtcaggat gatgtactcc
    14581 agagacatgg cgtaaacgcc ctgaagtgca aaaataacga cgctaatcac gttaataatc
    14641 acccacaaca gccagttttc cacatactta cgcgtcatca gaatcattgc cacgatagat
    14701 aacaccatca tgcatgaatc ccagaacggg aaagcgtccg gttgcagttc aggcatcaca
    14761 acctgtaatc ctaatgcttg catgatcatg actgccacgc gggtcaaaaa tgcaaacacc
    14821 ggattgataa ataccgtcat cagaccaatc gaaacaacgc aaaccgccaa ccagctgagc
    14881 gccttcggca atggtaacca gcgaattttc aactccgcct cgttctgact ggtttgtcgc
    14941 gaccacgcat accaaccgta aatattcgcg gcaaagaaaa acacctgtaa tagcaggctg
    15001 gcatacagct gaatctgaaa gaaaataatg ccaaacaagg tgacgttgat caggccaaag
    15061 aagtagttgc tgatcttctc cagactggca aggccaatac acagcaaccc ggcgatcgtg
    15121 cctaccgctt cgatccatga gagatcataa ccgcctgccc ctattggtat atgtaccagg
    15181 atattctgca cactaaaaaa atccatcttt cccccaaagc gtaacgttac gccttattat
    15241 tcgttatcca cgtagtgtag ccgcaaaatc cagcatacga ttgagcggca ccagcgccct
    15301 ctctcgcagc ctttcatcaa gaacctcgtg attgcttcct tcctgttcta atgcctctgc
    15361 gatggactga aggccattca tggccatcca cggacaatgc gcgcag
//
LOCUS       DAESHI010000100        15337 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.101, whole genome shotgun sequence.
ACCESSION   DAESHI010000100 DAESHI010000000
VERSION     DAESHI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.101"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            71..1015
                     /locus_tag="KW011_003194"
                     /pseudo
     CDS             71..1015
                     /locus_tag="KW011_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1051..1737
                     /gene="mlrA"
                     /locus_tag="KW011_003195"
     CDS             1051..1737
                     /gene="mlrA"
                     /locus_tag="KW011_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HBH7661464.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1797..1904
                     /locus_tag="KW011_003196"
     CDS             1797..1904
                     /locus_tag="KW011_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HBH7661465.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="KW011_003197"
     CDS             complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="KW011_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HBH7661466.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="KW011_003198"
     CDS             complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="KW011_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HBH7661467.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="KW011_003199"
     CDS             complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="KW011_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HBH7661468.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="KW011_003200"
     CDS             complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="KW011_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HBH7661469.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="KW011_003201"
     CDS             complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="KW011_003201"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HBH7661470.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8366..10081
                     /gene="dld"
                     /locus_tag="KW011_003202"
     CDS             8366..10081
                     /gene="dld"
                     /locus_tag="KW011_003202"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBH7661471.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="KW011_003203"
     CDS             complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="KW011_003203"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HBH7661472.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11226..11813)
                     /locus_tag="KW011_003204"
     CDS             complement(11226..11813)
                     /locus_tag="KW011_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HBH7661473.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11983..12561
                     /locus_tag="KW011_003205"
     CDS             11983..12561
                     /locus_tag="KW011_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBH7661474.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12691..13452)
                     /locus_tag="KW011_003206"
     CDS             complement(12691..13452)
                     /locus_tag="KW011_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBH7661475.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13505..14941)
                     /gene="mdtQ"
                     /locus_tag="KW011_003207"
     CDS             complement(13505..14941)
                     /gene="mdtQ"
                     /locus_tag="KW011_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HBH7661476.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            15165..15248
                     /gene="yohP"
                     /locus_tag="KW011_003208"
     CDS             15165..15248
                     /gene="yohP"
                     /locus_tag="KW011_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HBH7661477.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         3743 a         4176 c         3837 g         3581 t
ORIGIN      
        1 aaaaatatca tcagatggct gcttaaaaaa agaacaaatg gtagtgtccg ctattgccag
       61 tacacctcag ttgccaatgt taggtgaaaa aagactggtt tcatctgtaa caaaagagga
      121 tttgcttttc gtaaggagag atctgttgac cggttatcaa aagctttcta acggaaaaat
      181 ttcttccata aaaggacgct cagtggtcac agtaaactac tatatgacaa ccatagctgg
      241 aatgtttcaa tttgcaacag ataatggtta tacatcagga aatccattta acggtctgac
      301 accattaaaa aagtccaaga tagaaccaga tcccctcacc cgtgacgaat ttattcgttt
      361 tattgaggct tgccgtcatc aacaaacaaa aaacctgtgg attatcgctg tatacacggg
      421 tattcgtcac ggggagctgg tatcactggc ctgggaagat atagacctta aagcgaggac
      481 tataaccatc cgccggaatt atacaaaact aggcgaattc actccaccaa aaactgatgc
      541 tggtaccgga agaacgattc atctagttca accagctatt gatgctctta aaagccaagc
      601 ggaaatgacc atgcttggaa aacagcattc tgtagaggta aagcagaggg aatatgggag
      661 aagtactgtg cataaatgca cttttgtttt tagtcctcag gtaataaaac agcggcagtt
      721 ttccggaccg cactataagg ttgactccat cagggagtca tggacaagta tcttaaaacg
      781 cgcaggtctg agacacagaa aatcgtacca atccaggcat acttatgcat gctggtcact
      841 tgccgctgga gctaatccta gttttatcgc aagccagatg ggccacacaa acgcacaaat
      901 ggtattcaat gtttacggag catggatgaa agacaacaat cacgaacaga tagaactcct
      961 taacaaaaga ctatctgaaa gtgtcccatg tatgccccat aagaaagtgg ggtaaaataa
     1021 aaacttgtaa aatcagttag tttaccctta atccctgtca cgttacgcgc gtggcagagg
     1081 cgttacgggt tgctgaaacc gcaacggaca gacggcggtc atcgactgtt caacgatgcc
     1141 gatattgacc ggatccgcga gatcaaacgc tggatcgaca acggcgtgca ggtcagcaaa
     1201 gttaaaatgc tgctcagtaa tgaaaatgtt gatgtgcaga acggctggcg cgatcagcaa
     1261 gaaacattac tgacctacct gcaaagcggc aatctgcata gcctgcgaac gtggatcaaa
     1321 gagcgcggtc aggattaccc cgcccagaca ctcaccacac atctgtttat tcctctgcgc
     1381 cgacggcttc agtgccaaca accgactctc caggcgctgc tggcgatcct cgacggcgta
     1441 ctgatcaact acatcgccat ttgtctggct tcggcacgta aaaaacaggg taaagatgcg
     1501 ctggtggttg gctggaatat tcaggatacc acccgtctgt ggctggaggg ctggattgcc
     1561 agtcaacaag gatggcgcat tgatgtcctc gcccactcgc tcaatcaact acgccctgaa
     1621 ctattcgaag gccgtacatt gctggtgtgg tgcggtgaaa atcgaacctc cgcccaacag
     1681 cagcaactca ccagttggca agaacaaggc catgatattt tcccactcgg catttaatga
     1741 ttcgttaaca aatgcgcttt actgtacaat cctttcgtta acataaggag tgcaatatgc
     1801 gcatagctaa aattggggtc atcgccctgt tcctgtttat ggcgttaggc ggaattggtg
     1861 gcgtcatgct cgcaggttat acctttattt tgcgtgctgg ctaagcgcct gcaccagcct
     1921 ttcaaacagg cggtctgcga tgatcgccgc cagtgccacc agtaacgccc cctggatcac
     1981 atacgcggta ttaaatccgc taagcccgat gatgatgggc gtacccagcg tgctggcccc
     2041 taccgttgag gcgatcgtcg ccgtaccaat gttgataatc accgaagttc gcacgcccgc
     2101 cagaatcacc ggagccgcca gcggtagctc gaccttacgc agtcgctgac cacgactcat
     2161 tcccatacct ttcgcaactt ctgtcacgct ggcatcaatc gctcccagcc cggcaagtgt
     2221 cgcctgcagg acgggcagca caccgtaaag gatcaaggcg ataatcgctg gttgcagacc
     2281 aaagccgatc accggaacgg caatcgccag cactgcgaca ggcggaaaag tctgtccaac
     2341 ggcggcaata gtttccacca gtgggcgaaa ttccgcgccc cacgggcgag tgacagcaat
     2401 tccggcacca gtgccaatga tcaccgcaaa caaactcgaa attcccacca gccagaaatg
     2461 agccagtgcc agagctgcaa aactttcttg ctgataaacg ggtcgtggca gttgtgggaa
     2521 caaggcagca aacagcggct ggctgtaagg cagccagaaa atcagtgcca caaacaaagc
     2581 aatgagccag aacagcggat cgcgcaacat cttcatacgc ttacgcctcc tccagcagat
     2641 cctgaaaatg cagcgtgccg caaggctggc cctgcgtgtt caccaccggc agcacctcgc
     2701 atccccgcgc gacaaacagg gagagcgcat cgcgtagcgt catctcttct gccagtgcct
     2761 caccttctgc ccgttcttcg cgacgcacgt aatccgccac actacgtaac gaaagcaggc
     2821 gcacacccag ttcgctgcgc ccaaaaaact ggcggacaaa atcgtttgcc ggacgagtca
     2881 gcatcgtcag cggattgccc tgctgcacca cttcaccgtg atccatcaat accagatgtt
     2941 ctgccagccg tagcgcctca tcaatatcat gagtgaccag cacaatggta cgccccagca
     3001 aacggtgaat gcgcgtcatc tcttgttgca acgcgccgcg cgttaccggg tccagtgcgc
     3061 caaaaggttc atccatcagt aagacttgcg gatcggcagc cagtgcgcgc gccactccca
     3121 cacgttgctg ctgaccaccg gaaagctgat gcggataacg ctcacgtaaa tttgactcca
     3181 gccccagtag cgccattaat tcgtcgatac gatcgtcaat cctcgcccgc gaccattttt
     3241 gtaattgcgg cacggtagcg atgttttgcg ccacgctcca gtgggggaac aggccaatag
     3301 attgaatggc atagcccatc cggcggcgca actccagtac tggcagcgag cgaatttctt
     3361 ctccggcaaa gcggatcact ccgctgtcat gctccaccag gcggttaatc attttcaggg
     3421 tggtggattt gccggagcca gatgtgccaa tcagcaccga aaaactccct tcctgaaaat
     3481 tgagattgag atcgttaacg gctttttgtg cgccgaacag tttgctgaca tggccaaatt
     3541 caatcattac gtttcacctt cagcagtgcg ataagcaaat cgaacagcgc gtcggtaagc
     3601 accgccagaa caattaccgg gatcaccccc agcaacacta aatcaatggc gctgcttagc
     3661 agcccctgga aaaccagcgc accaaaaccg cctgcgccga ttaacgccgc aatcaccgcc
     3721 atacctacag tttgcaccat caccacccgc aggctgcgca gaaataccgg taacgccagt
     3781 ggtaactgaa catgcaggaa tcgctgcgcc ccgctcatgc ccatcgctct ggcgctctcc
     3841 agcacatcgc gcgggatctg gttcaaaccg actaccacgc cgcgcaccag cggcagcaag
     3901 gcatagagca ccagcgcaat cagtgcgggt gtcattccgg ttcctgctat gccgagcgtc
     3961 cccagccacg gaaaggccgt aaccagcgcg gcaagcggcg caatcaacag gccaaagagc
     4021 gccaccgaag gcacggtctg aatgacattg agcagagaaa aaattgcccc ctgccgagca
     4081 gtggaaaagt agcaccagat gcccaacggc acaccaatca ctaacgcagg cagcaccgca
     4141 ccaaacagca acgtcagatg ttgtgccagc gcgtcgtcaa acacatcctg acggttggcg
     4201 tattctttca ttagtgagag atcgttaagc gtgccggagt acagcaacca cagcggaata
     4261 atggcaatct gcatatgcaa caaccagcgc cacagcggat gcgtggagat tcggcggatg
     4321 gcatcactac aggccagcaa tgccagcgcc gcggccagcc agaaaccact gccgaggctg
     4381 gtacgcgcca gcgcactgcc attttgcgcc agttgggtcg ccgcctttcc tgctccccac
     4441 accagcaata cgaagacgaa ttgcgccaga atgagtgcaa aaatgctgcc ttttttagcg
     4501 ggaataaaac aggctgtcag ccaggcgcaa ccaacgccca ccagcatcca gatcgtttgc
     4561 ggccacagtt gccagagatg acgcccttca cccgagacta aacgattagg cgcgtaactg
     4621 ataaacggca gcgccgctgc gattgccgtc agcaacagca gcagcgccag aacaggatta
     4681 atacgtagat aagtcacggg aaattacttc gtccaccctt tttgtttcag gtagtcggca
     4741 gccacttttt tggcatccag tccttccact gcaatgctgg cattcagttg ctgcaatgtt
     4801 tttgcatcga ggctggcgaa gactggctgt agccactgtg ccatttgcgg atactctttc
     4861 agcaccgact cacgcaccac tggtgcaggc gcgtagatag gttgcacgcc tttcggatcg
     4921 cttaaggttt gcagccccag cgccgcgacc ggaccgtcag tgccgtaagc cattgcggca
     4981 ttaacgccag aagtttgctg ggcagcggct ttaatcgtta ccgccgtgtc gccgccagcc
     5041 agtgacagta actgatcctg atcgagttta aagtcgtagg ctttttcaaa cgcgggtaac
     5101 gcatcggcgc gttcgataaa ctctgccgag gctgccagtt tgaaggtgcc gccctctttc
     5161 agataacgac tcaggtcggc aagcgaagtg agtttgtttt tctctgccac atcctgacgc
     5221 acggcgatgg tccaggtgtt attcgctggt gcgggcgtca gccagattaa cttgttttgc
     5281 tctgcatcga gttttttgac tttctcgtaa ccttgctgcg cgtttttcca tgccgcatcg
     5341 ttttcatctt taaagaagaa agcgccatta ccggtatatt ccggatagat atccagttca
     5401 ccggaagtaa tcgccccgcg caccacagga gtcgttccga gttgcacttt attcaccgtc
     5461 ggtacgccgt ggctttccag cacctgcaag atgatgttgc cgagtagcgc gccttcggta
     5521 tcgatttttg aaccgacttt aacgggggaa gccgcctgta gcggcaggct cacggctgcc
     5581 aacataacca gtgaacctgc ccagaccttt gagagtggca tgatgctttc ctcattcttt
     5641 tactgttgtt ttcagcgaat taagagaaaa gcatagttga taatgacaga gttagcctga
     5701 gcggaaggat tcagttgcag aatcagataa atacttaaag ggcatatttt gcccggcgtt
     5761 tcttgccgga tgcggcgtaa acgccttatc cggcctacaa aggtcgaaaa ttcagcaagt
     5821 taagatatga acatcaggca aagggcgcaa acgtcgcgcc cttactaaag cattacagca
     5881 actcaaactc gcctttctta acgcgtgcgg aatcagtgcc gataaagaca ttgaacttgc
     5941 caggttcggc gtcatatttc atctgttgat tccagaactt cagcgcctcg atatcaatcg
     6001 ggaagctgac agtctgagtt tcgcccggtt tcagggtgat tttctcaaag cctttcagct
     6061 gcttcactgg acggctcatg gaagccgtca catcctgcaa gtacatctgt actaccgtgg
     6121 ccccttcgcg tttaccggtg ttcgtcacct gcacgctggc ggtcactttg ccgtcacgct
     6181 tcatggtcgg cgcagaaagt ttcacatcag agacggtgaa agtggtatag ctcagaccat
     6241 agccgaacgg ataaagcgcc ccgttagctt catcaaaata acgcgaagtg tatttgttcg
     6301 gcttgtcggc attatacgga cgaccggtat tcagatggct gtagtacacc gggatctgcc
     6361 cgacagaacg cgggaaggac attggcagct tgccggacgg gttgtaatcg ccaaacaata
     6421 catcggcaat tgcattaccg ccttcagtcc ccgcaaacca ggtttccaga atcgcatcag
     6481 cctgctgatc ttctttcacc agcgccagcg gacgcccgtt catcagcacc agcaccagcg
     6541 gtttaccggt ggctttcagc gccgcaatca agtcacgttg gctttgcgga atagtgatat
     6601 cggtacggct ggaggcctcg tgcgccatcc cctgtgcttc accgaccaca gccaccacca
     6661 catcagattg tttcgccgtc tgcaccgctt catcaatcat ctcttgcggc gagcgcggat
     6721 cgactttgac cgcttcttca tactgattca ggaaatcgat aatgcctttg tcactggtaa
     6781 cgttcgcccc tttggcatac agcactttac cgttttcacc gacggcattt ttaatcccgg
     6841 tcagtacggt cacggattga tcggcaacac cggctgcgga ccagctgccc atcacgtcac
     6901 gtttactgtc cgccagtggc ccaaccaccg caatggtggc cgattttttc agcggtaacg
     6961 tttcgagacg gtttttcagc aacaccaggc tttcgcgcgc cacttcacgc gcttctttac
     7021 ggtgcaggcg gctttcggca ttggtatcca ccgggtcaga ctctttcggc cccaaatggc
     7081 tgtatgggtc gttaaacaat cccatatcat atttaacgtt cagtacatgg cgggcagcat
     7141 cgtccagctc ttccatcgtc actttgccgg atttgatcaa cccaggcaga tacttcgagt
     7201 aatactcgtc gctcatactc atgttgattc cggatttcag cgccacgcgc accgcatctt
     7261 ccgggtctgc cgccgtgcca tgtttaatca gctctttgat tgcaccgtga tcggaaacgg
     7321 tgatgccttt aaagccccac tggtcgcgca gaacatcttt cagcagccag gaatcggagg
     7381 tggctggcgt gccgttcagc gagttcagcg ccaccatcac cgcgccgctg cctgcgtcca
     7441 gccccgcttt gtacggcggc atataatcat taaacaggcg ctgcggactc atatcgacgg
     7501 tgttgtactc tttaccgcct tctaccgcgc cgtatgcggc aaagtgtttg acgctggtca
     7561 tcaccgagta gcgatctgcc gggcttttac cctgcatcgc ttccaccatg gttttaccca
     7621 ttgttgaggt gagatacgta tcttcgccaa aaccttcgga agcacgtccc cagcgcggat
     7681 cgcgcgagac atcgaccatc ggtgcccagg tcatattcag gccatcatct gccgcttcat
     7741 aagcagagac acgtccgacc gttttcactg catcgaggtt aaaagacgag gccagaccga
     7801 ggctaatcgg gaacaccgtg cgctgaccgt gcagcacgtc gtaagcaaag aaaagaggaa
     7861 ttttcaggcg gcttaattcc atcacctgat cctgcatggc gcggatatcc tgacgggtta
     7921 cggtgttgaa aatcgcccca acctgaccgt ctttgatcat ctcgcggatc gcctctttcg
     7981 ggttatccgg accgacgctg attaagcgca gctgaccaat tttctcatca actgtcattt
     8041 tcttaagcag ttcggtgacg aacgcatccc gcgcttcggg cgttaatgga tggttgccga
     8101 ataaatcatc cgccagtgct ggctgcaggg ccagactcac cgcgattcct actgaacata
     8161 gccatttcat atggattttt cttcctctgt tgcccgacga ggcagcgaaa atggtgcaaa
     8221 aaccgtagtt tgccataagc atgatggaga gagaaaaaga atgctcagtt tattgtctga
     8281 attttcaaaa tattcactcg ctgaattgtc atacaaggcg ctattctagt ttgtgatatt
     8341 ttttcgccac cacaaggagt ggaaaatgtc ttccatgaca acaactgata ataaagcctt
     8401 tttgaatgaa cttgcccgtc tggtcggtca ttcacacctg ctcaccgatc ccgcaaaaac
     8461 ggcccgctat cgcaagggct tccgttctgg tcagggcgac gcgctggctg tcgttttccc
     8521 tggctcacta ctggaattgt ggcgggtgct gaaagcctgt gtcaccgccg acaaaattat
     8581 tctgatgcag gctgccaata caggcctgac cgaaggatcg acgccaaacg gtaacgacta
     8641 tgatcgcgat atcgtgatca tcagcaccct gcgtctcgac aagctgcacg ttcttggcaa
     8701 gggcgaacag gtgctggcct atccgggcac cacgctctat tcgctggaaa aagccctcaa
     8761 accgctggga cgcgaaccgc actcagtgat tggatcatcg tgtataggcg catcggtcat
     8821 cggcggtatt tgtaacaact ccggcggctc gctggtgcaa cgtggcccgg cgtataccga
     8881 aatgtcgtta ttcgcgcgta taaatgaaga cggcaaactg acgctggtga accatctggg
     8941 gattgatctg ggcgaaacgc cggagcagat ccttagcaag ctggatgatg atcgcatcaa
     9001 agatgacgat gtgcgtcacg atggtcgtca cgcccacgat tatgactatg tccaccgcgt
     9061 tcgtgatatt gaagccgaca cgcccgcacg ttataacgcc gatcctgatc ggttatttga
     9121 atcttctggt tgcgccggga agctggcggt ctttgctgta cgtctagata ccttcgaagc
     9181 ggaaaaaaat cagcaggtgt tttatatcgg caccaaccag ccggaagtgc tgaccgaaat
     9241 ccgccgtcat attctggcta acttcgaaaa tctgccggtt gccggggaat atatacaccg
     9301 ggatatctac gatattgcgg aaaaatacgg caaagacacc ttcctgatga ttgataagtt
     9361 aggcaccgac aagatgccgt tcttctttaa tctcaaggga cgcaccgatg cgatgctgga
     9421 gaaagtgaaa ttcttccgtc cgcattttac tgaccgtgcg atgcaaaaat tcggtcacct
     9481 gttccccagc catttaccgc cgcgcatgaa aaactggcgc gataaatacg agcatcatct
     9541 gctgttaaaa atggcgggcg atggcgtggg cgaagccaaa tcgtggctgg tggattattt
     9601 caaacaggcc gaaggcgatt tctttgtctg tacgccggag gaaggcagca aagcgttttt
     9661 acaccgtttc gccgctgcgg gcgcagcaat tcgttatcag gcggtgcatt ccgatgaagt
     9721 cgaagacatt ctggcgttgg atatcgctct gcggcgtaac gacaccgagt ggtatgagca
     9781 tttaccgccg gagatcgaca gccagctggt gcacaagctc tattacggcc attttatgtg
     9841 ctatgtcttc catcaggatt acatagtgaa aaaaggcgtg gatgtgcatg cgttgaaaga
     9901 acagatgctg gaactgctac agcagcgtgg cgcgcaatac cctgccgagc ataacgtcgg
     9961 tcatttgtat aaagcaccgg agacgttgca gaagttttat cgcgagaacg atccgaccaa
    10021 cagtatgaat ccggggatcg gtaaaaccag taagcggaaa aactggcagg aagtggagta
    10081 aaaattacgg atggcatagt atcgccatcc gtcattcact taatcgttct gtgccgtctg
    10141 ccccgccgcc gccatttggg cggctttttg ttttttatag ctcaacgctg ctgctggaac
    10201 aggcatcact ttaccggttt caatccaggt acgcaggcgg ctggcatcgg caaaatgggt
    10261 atatttgcca aacgcatcca tcactaccag cgccaccggt ttattattga taaccgtacg
    10321 catcaccaga caatggcccg ccgcattggt aaaaccggtt ttggttaact gaatattcca
    10381 gttatcgcga tacaccagat ggttagtatt gcggaacggc agcgtatacg tcgggttaga
    10441 gaaggttgcc atatcttccc gggtagtact taactgcccg atcaacggat attgtttgct
    10501 ggcaatgagc agtttggtta agtcacgggc agttgaaacg ttatgcaccg acaatccggt
    10561 aggttcaaca aagcgcgtgt tgttcattcc gagcgatttc gctttcgcat tcattgcctt
    10621 aataaaggct ttgtaaccac cgggataatg gtgtgcaagg cttgccgccg cgcggttttc
    10681 tgaagacatc agcgccagca acagcatatc tttacggctg atttcgctat tcagtcgtac
    10741 gcgcgaatag acccctttca tctccggcgt ctggctgata tccactttta gtttttcatc
    10801 cagcggcagt cgtgcatcca gcacaaccat cgcggtcatt aatttgctga tagacgcaat
    10861 cggacgcacc agatccgggt ggttcgagta gatcactttg ttggtattca gatcaacaat
    10921 catcgcgcta ccggaggcaa tttcaggttg tgaagcggtg gtagcggctg ccgttttcgc
    10981 aacggcctgc ggtgccaaag gcacagccag catcagggcc aggctaaata aagaaactcg
    11041 aaatttcggc atgatgagca ttcagatagt ggttcacgcg cacaggttgc gcaccgccgg
    11101 agtaaggatt tactgaggct agcgacgcca tcataacgag caaaaagtgc gatcgtcaaa
    11161 ggagaatcgt gaggaaatgc tgcattgctg acatttacgc cagcaatgca acgtcaaaga
    11221 actttctaga acaaacgata accgtagccc catagtataa cggttagggc gagcagcact
    11281 tccagtacca gcacgccaat cgccagcgtc gaactagaga agctaaggcc ttcctctttg
    11341 ttgatattca ggaagctcgg aataccaagg taaagcaggt agccggtgta aaacagcgcc
    11401 accgtgccga ccagcgcaca caaccagacc agtggataaa gcgccaccag accgcttaaa
    11461 aacagcggag ttgcaacgta gcccgcgaag accatgcagt gcgcaagtga cggacgctgc
    11521 ggataattac gcgccatcca ccagatgacc cgccccatta ccgcaacccc agccagcata
    11581 acgccataaa acaggacagc cagcgccagt ccggtaaacc aggataactt caggatagtg
    11641 ccatcgccaa aattccagcc aatctgtgta gtgccaatga aggcgcaaat caccggaatc
    11701 gccgccatca gcaaaacgtg gtgggtgtaa tggtgagaaa tcgtttcgtt ttcgcgatta
    11761 atcacctgca tttcacgatc gggatgggaa aacagtcccc agacatggct cataccgccc
    11821 ccttgttgtg agttcatgaa cctgacagtt caagtataag tcagcttgtg attatttttt
    11881 gttcgctcat gtaatttgca ccgtctgatg tacgtccttt ttcagcgtga atgatttcca
    11941 gggtgtatgc ttacaggcaa ccaaagggag acagactggc ctatggatct caatacactc
    12001 atctcacaat atggttatgc cgcgctggtg atcggtagcc tggcggaagg tgaaaccgtg
    12061 actttgctgg gaggcgttgc ggcacatcag gggctattaa agttcccgct ggtggtactt
    12121 tctgtggcgc ttggcggcat gattggcgac caggtgcttt atctgtgcgg gcggcggttt
    12181 ggcggcaagc tgttacgccg tttctcgaaa catcaggata aaattgagcg ggcgcagaaa
    12241 cttatccaac gccatccgta tctgtttgtc attggtacgc gctttatgta tggctttcgg
    12301 gtgattggcc cgacgctgat tggtgccagc cagctgccgc cgaaaatctt tctgccgctg
    12361 aatattctcg gcgcatttgc ctgggcgttg atttttacca ctattggtta cgctggtggt
    12421 caggtgattg cgccgtggtt gcacaatctc gaccagcatt tgaagcactg ggtctggttg
    12481 attctggtcg tggttctggt ggtgggcgtg cgctggtggc tgaaacggcg cgggaagaaa
    12541 aagccggatc atcaggcgta aaaccattgc cctgggtaag gcgttcgcgc cgcatccgac
    12601 atccggtgca ccatgcctga tgcgacgctt gtgcgtctta tcatgcctac caaaatttta
    12661 cccattgaaa taaccacgca cctcactgcg ctattctggg ttgaactgcg gattcgccaa
    12721 cataaagccg ccatccacta tcaacgactg cccggtggtg taatttgcgc cctctgagca
    12781 cagccacgct accaggctgg caatctcatg cgttgcgcca aaacgccgca gaggaatcga
    12841 aggctccgcg tcgggcttca cgtcgctgtc atccatgcca ttcattggcg tggcgatcgc
    12901 cccaggcgcg actgcgttca ccaaaatctt atgcctgacc agctccagcg ccatcgtttt
    12961 ggttaacccg ccgagcgcat gtttagcggc tgtgtaagcg ctggcatccg gcagcggcgt
    13021 atgttcatgt accgacgtaa tgttgatgat acgaccgccc tgcccttgtt tcaccatctg
    13081 gcgggccgca atttgtgagc ataaaaatgc accatcgaca tcaacggtaa aaatcttgcg
    13141 ccactcatcg aaagccatat caagaaacgg cgctttggtc attgcgcccg cattattcac
    13201 cagcacatca atacgcccca gccgttgaat gagtttctcc agcgcctgcg caccttctgg
    13261 cagtttaccg agctccagct gcacgatctc cgcacgtacg ccgtggctaa ctacctcacg
    13321 cgcggtatct tttgcccctt cttcatctga gtgccaggta atgccaatat caaacccctg
    13381 ctgcgccagt aataacgcgc actctttgcc gatccccgaa tcggaggcgg taataatcgc
    13441 aacctgtgcc atcaagttct ccacttaacg ctgaataaac gttaagtata gaaggcgcat
    13501 atcatcagcg tttgtaaccc ccgcccaacg caccagtgag ttgaatagaa gcatccagcc
    13561 actgcccttg caataacagg ccattggccc gctcacgcag cgcggggatt ctggcttcgc
    13621 tgacgcggga accagcaatg atgcccgcgt taaagcgcgc ctgcgcaaga cctaccacac
    13681 gcaaggcatc gcgctcaatt tgcgcctgat gctggttttt ctccgcaagt gtctgaacct
    13741 gactggctgc ccgcgccacg tcattcaccg cttcaaccac cgctttgttg tagctggcga
    13801 tagacaagtt gctttcggct tttgcgatat tgagattggc gttaagacga ccactatcga
    13861 aaatgggtag cgtcaggcct gccgtaacgc ccatttgctg cgcggaatga cggaacagat
    13921 cgcttaagtg caacgcatcc tgttgcagga aggccatcag gttgatgtca ggataaaatg
    13981 ccgcttttgc cgcatcaatg gtgcttagcg atgactcaac gtaccagtgc gccgcctgca
    14041 aatctgcccg ccgggccagt aaggagtacc ccagttcatc aggaagctgg cttgccactt
    14101 tcggcaacgc gaccggttta agcttcaatg actttgtctg gttatttgta agtgcgctta
    14161 accgtgcctc aataattttc attttccccg cgacatcgtt gagctgctgc cgggttttgc
    14221 tggcattaat atcggtttcc acaccttcaa ctgaagaagt aatcccgttc tgatatagct
    14281 ggcgatcagt cgcgataatg gtgttctgct ctttttctat ttgctgcaag accgtgttta
    14341 acgccgcctg ggtttgccac tcccagtaca ggcgggctac gctgccagcc agcaattggc
    14401 gggtttgctc gcgttccgcc gcccgtgctt taaccgtacc caggcgggca gtaacctccg
    14461 cccgattctt tccccagata tcgagatgcc agcccgccgt taagccaaaa gtaccgttgg
    14521 tgtaccacgg gccggtcgta cctgcggccg gatcgttcag agcaaacggc cccattaagc
    14581 cttctgccga cattttttgc cgctccatat ccgccgaaaa gtcgatctgc ggaccatcct
    14641 gagtggcaac tgccttcgcc tgggcttcag ctagctgaat gcgctgttca gccacctgca
    14701 tatccggtgc gttctgtagt gcattgttaa ttaatgaagt gagctgatta tcgtgatact
    14761 ccagccacca ttggctgtct ggccaaccat ttttcagcgc cgtgggtaat gcggtgtcaa
    14821 cttgtgcagc gggcgtttgc tggcttaacg cctggcgggt ttcatgcata ggcgcacacc
    14881 cggccagcat cagtaacagc ggaaaacagg cgatggctgg ataaaaggaa tcacgattca
    14941 tgggggaata atcaggtaag aaacggtgcg cggagattac cgtgtgttgc gatatatttt
    15001 ttagtttcgc gtggcaatac atcagtagca ataaaacgac atacccagaa aaatatagac
    15061 taagtaaatg atatcttccg atttatctta atcgtttatg gataacggca aagggcttcg
    15121 ttttttccta tacttattca gcactcacaa ataaaggaac gccaatgaaa attatactct
    15181 gggctgtatt gattattttc ctgattgggc tactggtggt gactggcgta tttaagatga
    15241 tattttaaaa ttaattaatg tcatcaggtc cgaaaataac gagaatattt cagtctctca
    15301 tcctgttgcg ctcctgtcat gtgcattgct tcatata
//
LOCUS       DAESHI010000101        15181 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.102, whole genome shotgun sequence.
ACCESSION   DAESHI010000101 DAESHI010000000
VERSION     DAESHI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15181
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.102"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            1..776
                     /locus_tag="KW011_003209"
                     /pseudo
     CDS             1..776
                     /locus_tag="KW011_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
     gene            1099..2487
                     /gene="narU"
                     /locus_tag="KW011_003210"
     CDS             1099..2487
                     /gene="narU"
                     /locus_tag="KW011_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HBH7661478.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIDDQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            2569..6309
                     /gene="narZ"
                     /locus_tag="KW011_003211"
     CDS             2569..6309
                     /gene="narZ"
                     /locus_tag="KW011_003211"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HBH7661479.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            6306..7850
                     /gene="narH"
                     /locus_tag="KW011_003212"
     CDS             6306..7850
                     /gene="narH"
                     /locus_tag="KW011_003212"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBH7661480.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7850..8545
                     /gene="narW"
                     /locus_tag="KW011_003213"
     CDS             7850..8545
                     /gene="narW"
                     /locus_tag="KW011_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBH7661481.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            8542..9222
                     /gene="narI"
                     /locus_tag="KW011_003214"
     CDS             8542..9222
                     /gene="narI"
                     /locus_tag="KW011_003214"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBH7661482.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            9301..10194
                     /locus_tag="KW011_003215"
     CDS             9301..10194
                     /locus_tag="KW011_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HBH7661483.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(10290..11135)
                     /gene="nhoA"
                     /locus_tag="KW011_003216"
     CDS             complement(10290..11135)
                     /gene="nhoA"
                     /locus_tag="KW011_003216"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HBH7661484.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            11308..11877
                     /locus_tag="KW011_003217"
     CDS             11308..11877
                     /locus_tag="KW011_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBH7661485.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11881..12108)
                     /gene="pptA"
                     /locus_tag="KW011_003218"
     CDS             complement(11881..12108)
                     /gene="pptA"
                     /locus_tag="KW011_003218"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HBH7661486.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(12208..13343)
                     /locus_tag="KW011_003219"
                     /pseudo
     CDS             complement(12208..13343)
                     /locus_tag="KW011_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(13483..14619)
                     /locus_tag="KW011_003220"
     CDS             complement(13483..14619)
                     /locus_tag="KW011_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HBH7661487.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSTDD
                     KDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEKKFPLTELNNP
                     AHDSSAMSEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKE
                     PEMMVRYYISSADLTAEKFATAIRNHWHVENNLHWRLDVVMNEDDCKIRRGNAAELFS
                     GIRHIAINVLTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            complement(14976..15152)
                     /locus_tag="KW011_003221"
     CDS             complement(14976..15152)
                     /locus_tag="KW011_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661488.1"
                     /translation="MSFIFNEETLYFTVFGGDFIMDDLKIVKNTEAWVWYKNCDSKNK
                     KVAVELLQIYELHQ"
BASE COUNT         3780 a         3783 c         3619 g         3999 t
ORIGIN      
        1 gtttgtgcaa ttagaatctt gcgaatgtct gcaatacctg aatctcagcc ataatcaatt
       61 atctgatatt gttgcaggta ataaaaatta actcttactg ctggatctat cccataataa
      121 actaacaagt ttacacaatg tcttatttcc caacttgaat acgttactta ttaacaaaaa
      181 cctgctttct gaaattaaaa tattctatag caacttctgc aatgttcaga cattaaacgc
      241 tgctaacaac cagttgaaaa atataaatct tgatttcctg acttgtcttc catctattaa
      301 aagtttaaga ctggacaaca ataaaataac ccatattgat actaataata catccgatat
      361 tggaacttta tttcccataa taaaacagag caaaaactta aattttttaa atgtttctgg
      421 gaagaacaat tgccctacta tgcagctcat gttatttaat ttgttttccc caacacttaa
      481 gcttaatact ggcctggcaa ttctttcgcc tggtgcattt gaagttcact ctgacggaat
      541 agatgtggat aacgaattgt ttcactatcc tattaaaaat agcatatacc ccatataata
      601 tacacactta caagacagag gaagttgtaa accagaggaa tataaaagtt aaaaacatga
      661 ccttagatga aataaacaat acttactgta ataacgatta ttacaatcag gcaataagag
      721 aggaaccgat agaccttctg gacagatcgt tttcctccag ttcatggcct ttttagtgaa
      781 taaatcatca ctaattctat aagtgtctgg tcttgttgat aatatagtta atatgtaacc
      841 ctttagattc acgatctgaa gggttgaaac atgacatacg ttcaaagatt aaccccgttt
      901 gaaggttagc ttgttagtta catttagtaa cacatatctg gctacttaat ccccattctg
      961 cccccgcggt tacgctttct tatctattct ttcaatcagc acttcaccca attcatcctt
     1021 aattctaata actggctatt gcgccttacc tcctgttttg tcttttttca ttaccaatgt
     1081 gtgcatgtga ggaacaatat ggcactgcaa aatgagaaaa atagtcgtta tcttttacgc
     1141 gactggaaac cagaaaatcc ggccttctgg gaaaataaag gaaaacatat tgctcgaaga
     1201 aacctctgga tatcagtcag ttgtctactt cttgccttct gtgtctggat gctatttagc
     1261 gcagttaccg ttaatctcaa taaaatcggt tttaatttta ctaccgatca actcttttta
     1321 ttaaccgcat taccctccgt ttctggcgca ttattgcgtg ttccctactc ctttatggtg
     1381 cctatattcg gtggacgccg atggacggtt tttagtactg caatcctgat tattccttgc
     1441 gtctggctcg gaattgccgt gcaaaatccg aatactcctt ttgggatatt tatcgttatc
     1501 gctttgctat gcggttttgc aggtgcaaac tttgcttcga gcatgggcaa tatcagtttc
     1561 ttctttccaa aagccaaaca agggagcgct cttgggatta atggcggatt aggaaactta
     1621 ggtgtaagtg taatgcagct ggttgcaccg ctggtcattt ttgtacctgt atttgccttt
     1681 ctcggcgtca atggcgtacc gcaggccgac ggttcggtga tgtcgctggc gaatgccgca
     1741 tggatttggg taccgctact ggcgattgcc acgatcgccg cctggtcagg gatgaatgat
     1801 atcgccagtt cacgcgcctc aattgacgac cagctccctg tcttacaacg cctgcatctc
     1861 tggctgctga gcctgcttta ccttgccacc ttcggttcgt ttatcggttt ttctgcgggt
     1921 tttgccatgc tggcaaaaac ccagttcccg gatgtgaata ttctgcgcct ggcgttcttt
     1981 ggcccattta tcggtgccat cgcgcggtcg gttggtggtg ctatttccga taaattcggc
     2041 ggcgtgcggg tgacgttgat caactttatt tttatggcga ttttcagtgc cctgctgttc
     2101 cttaccttac cgggcacagg ctccggtaat ttcatcgcct tttacgccgt atttatgggg
     2161 ctgtttctga ctgcgggtct gggaagtggt tctactttcc agatgatcgc cgtcatcttt
     2221 cgccagataa ccatttatcg ggtgaagatg aaaggcggta gtgatgagca agctcagaaa
     2281 gaagccatca ccgaaacggc ggcggctctg ggctttatct cagccattgg cgcagtgggc
     2341 ggctttttta ttccgcaggc gtttggcatg tcgctcaata tgaccggctc tccggtcggc
     2401 gcgatgaaag tgtttttaat cttctacatc gtttgtgtgc tgctgacctg gctggtttat
     2461 ggtcggcgga agttcagcca aaaataagag atcgatgcct gatgtacaac cgtgtcgcat
     2521 ccgacaaggc atcagatccc aattcattcc tggagcagga gtcatgtcat gagtaaactt
     2581 ttggatcgct ttcgctactt caaacaaaag ggcgaaacct ttgccgatgg tcacggacag
     2641 gtgatgcata gcaaccgcga ctgggaggac agctatcgcc agcgttggca gttcgacaaa
     2701 atcgtgcgtt ccacccacgg tgttaactgt acaggctcct gtagctggaa aatctacgtt
     2761 aaaaatggtc tggtgacctg ggaaatccaa cagaccgact acccgcgcac tcgccctgac
     2821 cagcccaatc atgaacctcg cggctgcccg cgtggcgcaa gttactcctg gtatctctac
     2881 agcgctaacc gcctgaaata cccgctcatt cgtaaacgac tgattgaact gtggcgtgaa
     2941 gctctcaagc aacacagcga tccggtactg gcgtgggcat cgattatgaa cgatccgcaa
     3001 aagtgcttaa gctacaaaca agtgcgtggg cgcggcgggt ttatccgctc caactggcag
     3061 gaacttaacc aactgattgc cgccgctaac gtctggacca tcaaaatcta tggtccggat
     3121 cgcgttgccg gtttctcgcc gatcccggcg atgtcgatgg tgtcatacgc tgccggaacg
     3181 cgttatctgt cgctgttagg cggcacctgt ttaagcttct acgactggta ttgcgacctg
     3241 ccgcccgcct cgccgatgac ctggggcgaa caaaccgacg tgccggaatc tgccgactgg
     3301 tataactcca gttacatcat cgcctggggg tctaacgtac cgcagacacg tacgccggac
     3361 gcccacttct ttaccgaagt acgttacaaa ggcaccaaaa ctatcgccat tacccctgac
     3421 tactctgaag tggccaaatt gtgcgaccag tggctggcac cgaaacaagg cactgatagc
     3481 gccctggcga tggcaatggg ccatgtgatt ttaaaagagt ttcatctcga taatcccagc
     3541 gactacttta tcaactactg ccgccgctat agcgacatgc cgatgctggt aatgctggaa
     3601 cctcgcgacg atggtagcta cgtacccggg cgcatgatcc gcgcgtctga cctggtagat
     3661 ggactaggcg aaagcaataa tccgcagtgg aaaaccgtgg cggttaatac cgcaggtgaa
     3721 ttggtagtgc cgaatggttc gattggtttc cgctggggag aaaaaggcaa atggaatctg
     3781 gaatccattg ccgccggtaa ggaaaccgaa ttgtcgttaa ccctgctcgg tcaacatgac
     3841 gctgttgcag gcgtggcctt cccctacttt ggcggcattg aaaatccgca ttttcgcagc
     3901 gtaaaacaca atccggtgct ggtgcgccaa ttgcccgtta aaaacctgac gttagtcgat
     3961 ggcaacacct gtccggtggt cagcgtttat gatttggtgc tggcgaatta cggcctcgat
     4021 cgcgggctgg aagatgaaaa cagcgcgaaa gattacgctg aaatcaaacc gtacacccca
     4081 gcctggggtg agcaaattac cggcgtaccg cgccagtata ttgaaaccat cgcccgcgaa
     4141 tttgccgata ccgcccataa aacgcatgga cgctcaatga ttatcctcgg cgctggcgtc
     4201 aaccactggt atcacatgga tatgaactac cgtgggatga tcaatatgct gatcttctgc
     4261 ggttgtgtcg ggcaaagcgg cggcggctgg gcgcactatg tcggtcagga aaaactgcgc
     4321 ccacaaaccg gctggttgcc gctggccttt gcgctcgact ggaaccgccc accgcgccag
     4381 atgaacagta cctcgttttt ctacaatcat tccagccaat ggcgctatga aaaagtctct
     4441 gctcaggagt tactttcacc gcttgccgat gccagtaagt acagcggtca tctgattgat
     4501 ttcaacgttc gcgccgaacg catgggctgg ctaccttctg cgccgcagct ggggcgtaac
     4561 ccgctcggga ttaaagctga agccgacaag gccggattat cccccacaga atttaccgcc
     4621 caggcgctga aatcgggcga tttacgtatg gcctgcgaac aaccagatag cggcagcaat
     4681 catccgcgta atttgtttgt ctggcgctct aacctgcttg gctcctccgg caaaggccac
     4741 gagtatatgc agaagtatct gctggggacc gaaagcggga ttcagggcga ggaactcggt
     4801 gccagcgacg ggatcaaacc ggaagaagtc gagtggcaaa ctgcggcgat tgaaggcaag
     4861 ctcgacctgc tggtgacgct cgacttccgc atgtccagta cctgcctgtt ctccgatatc
     4921 gttctgccca ccgccacctg gtacgaaaaa gacgatatga acacctcgga tatgcatccg
     4981 tttattcatc cgctttctgc ggcggtcgat ccggcgtggg aatcacgcag cgactgggaa
     5041 atctacaaag gtatcgccaa agcattttcg caagtgtgcg tgggtcatct tggcaaagaa
     5101 accgacgtgg tattacaacc cctactgcat gactctccgg cagagctctc acagccgtgt
     5161 gaagtgctcg actggcgcaa aggcgaatgc gatctgattc caggcaaaac cgcgccgaat
     5221 attgtggcgg tggagcgcga ctaccctgct acgtatgaac gctttacctc gctcgggcca
     5281 ttgatggaca aacttggcaa tggtggtaaa gggatttcgt ggaataccca ggatgaaatc
     5341 gatttcctcg gcaaactcaa ttacaccaag cgtgatggcc cggcacaggg gcgtccgctg
     5401 attgacaccg ccattgacgc ttcagaagtg attctggcac tggcaccgga aaccaacggt
     5461 catgttgcag tcaaagcgtg gcaggcgctg ggcgagatca ccgggcgcga acatacccat
     5521 ctggcgctgc acaaagagga cgagaagatt cgctttcgcg atattcaggc gcagccgcgt
     5581 aaaattatct ccagccccac atggtccggt ctggaaagcg atcacgtctc ctataatgcg
     5641 ggatacacca acgttcatga gttaattccg tggcgcacgc tgtcgggacg ccagcagctc
     5701 tatcaggatc atccgtggat gcgtgctttt ggtgaaagcc tggtggctta tcgcccgcct
     5761 atcgacaccc gtagcgtcag tgagatgcgc cagatcccgc caaacggctt cccggaaaaa
     5821 gcacttaact tcctgacgcc gcaccagaaa tggggcattc actcaaccta cagtgaaaac
     5881 ctgctaatgc tgacgctctc tcgcggtgga ccgattgtct ggatcagcga aaccgatgcc
     5941 cgagaactga ccattgtcga taacgactgg gtggaagtct ttaacgccaa cggcgcgctg
     6001 acggctcgtg cggtggtcag ccaacgtgta ccgccgggta tgaccatgat gtatcacgcc
     6061 caggaacgca ttatgaatat tcctggttcg gaagtaactg gcatgcgcgg cggcattcat
     6121 aactcggtca cccgtgtttg cccgaaaccg acgcatatga ttggcggtta cgcgcagctg
     6181 gcctggggct ttaactacta cggcaccgtc ggctcgaacc gcgacgaatt catcatgatc
     6241 cgcaagatga agaacgttaa ctggctggat gatgaaggtc gcgatcaggt acaggaggcg
     6301 aaaaaatgaa aatacgttcg caagtcggca tggtgcttaa cctcgacaaa tgtatcggtt
     6361 gccatacctg ttcggtgacc tgtaaaaacg tctggaccgg acgcgaaggc atggagtacg
     6421 catggtttaa caacgtcgaa accaaaccgg gcattggtta tccgaaaaac tgggaagatc
     6481 aggaagagtg gcaaggcggc tgggtccgcg atgtgaatgg caagatacgc ccgcgtctgg
     6541 gcagcaagat gggcgtaata accaaaatct tcgccaaccc ggtggtgccg cagattgatg
     6601 attactacga acctttcacc ttcgactacg aacatttgca tagcgcaccg gaaggcaaac
     6661 atatccctac tgctcgcccg cgttcgctga ttgacggtaa gcggatggac aaagtgatct
     6721 gggggccaaa ctgggaagaa ctgctgggcg gcgagtttga aaaacgtgcc cgcgaccgca
     6781 acttcgaggc catgcaaaag gagatgtacg ggcagtttaa aaacaccttc atgatgtacc
     6841 tgccgcgcct gtgcgaacac tgcctcaatc ccagttgcgt ggcgacctgc ccaagcggcg
     6901 ctatctacaa acgcgaagaa gacggcattg tgctgattga tcaggataaa tgccgtggct
     6961 ggcgtttgtg cattagcggt tgtccgtaca aaaaaatcta cttcaactgg aaaagcggca
     7021 agtcagaaaa atgtatcttc tgttatccgc gaattgagtc aggccaaccc accgtgtgct
     7081 cagaaacctg cgtgggtcgc atccggtatc tgggcgtgct gctttacgac gccgaccgca
     7141 ttgaggaagc ggcgagcacc gagcgcgaag ttgacctcta tgaacgtcag tgcgaagtgt
     7201 tcctcgatcc acacgatccc tcagtgatcg aggaagccct gaaacaaggt attccacaaa
     7261 acgtgattga agctgcccag cgttcgcctg tctacaaaat ggcgatggac tggaaactgg
     7321 cgctaccgct gcaccctgaa tatcgcaccc tgccaatggt ctggtacgtt cctccgctgt
     7381 cgccgattca gtcctacgca gatgcgggcg gtttgccgaa aagcgaaggc gtgctgcccg
     7441 ccatcgaaag cctgcgtatt ccggtgcaat atctcgccaa tatgttgagt gccggcgata
     7501 ccggtccggt actgcgggcg ctgaaacgga tgatggcgat gcgccactat atgcgttcac
     7561 aaaccgtgga aggcgttact gatactcgtg ccatcgacga agtaggcctg agcgtcgccc
     7621 aggtcgaaga aatgtatcgc tatctcgcca ttgccaacta tgaagatcgt tttgttatcc
     7681 cgaccagcca tcgggaaatg gcgggcgatg ccttcgcaga acgcaacggc tgcggtttta
     7741 cctttggcga cggttgccac ggttcggaca gtaaattcaa cctgttcaac agtagccgta
     7801 tcgatgccat caacatcacc gaagtgcgcg acaaagcgga gggcgaataa tgcagatcct
     7861 caaagtgatc ggcctgttga tggagtatcc ggatgagctg ttgtgggaat gcaaggagga
     7921 cgcgctggcg ttgatccgcc gcgacgcgcc gatgcttacg gatttcaccc acaacctgct
     7981 taacgcgccg ctgctggata aacaggccga atggtgcgaa gtgtttgacc gcgggcgcac
     8041 cacgtcgctg ctgctgttcg aacatgttca tgccgagtcc cgcgatcgcg gccaggcaat
     8101 ggtggacctg ctggcggagt atgaaaaggt cggcctgcag ctggattgtc gggaactgcc
     8161 cgattatcta ccgctgtatc tggagtattt aagcgtactg cctgacgatc aggcgaaaga
     8221 aggattgctc aatgttgcgc cgatcctcgc cctgcttggc ggtcgcttaa aacaacgcga
     8281 ggcaccgtgg tacgcgttgt ttgatgctct gctgcaactg gcgggaagta tactttcaag
     8341 tgacagtgtc acaaaacaag taaacagcga agagcgcgat gacacccgcc aggcgcttga
     8401 tgcagtgtgg gaagaggaac aggtgaagtt tattgaagat aacgccacgg cgtgtgacag
     8461 ctcgccgctt aatcaatatc agcgacgctt tagccaggat gtcgcgccgc agtatgtcga
     8521 catcagtgcg ggaggtggga aatgattcag tatctgaacg tcttttttta cgatatctac
     8581 ccgtacattt gtgcgacggt gtttttcctc ggcagctggc tgcgctacga ctacgggcag
     8641 tacacctggc gcgcctcctc aagtcaaatg ctcgataaac gcgggatggt gatatggtcg
     8701 aatctgttcc atatcggcat tttggggatt ttcttcgggc acttgttcgg catgttaacg
     8761 ccgcactgga tgtacgcgtg gtttttgcca gtggcagcga aacaactcat ggcgatggtg
     8821 ctcggcggta tttgcggtgt gttgacgttg attggcggcg caggactgct gtggcgcagt
     8881 ctgacaaatc agcgcgtgcg tgccacttcc actacgccgg atatcatcat catgagcatt
     8941 ttgctgatcc agtgtctgtt ggggctaagt accataccgt tttcagcaca gtatcctgat
     9001 ggtagcgaaa tgatgaagct ggtgggttgg gcgcaaagca ttgtcacttt ccgtggtgga
     9061 tcgtcagaga tgctcaacgg cgtagcgttc gtcttccgcc tgcatctggt gttgggaatg
     9121 accatcttcc tgctcttccc gttcacccga ctggtgcatg tgtggagcgc gccgtttgag
     9181 tactttactc gtcgatatca aattgtgcgt tcgcggcgat aatttcaccc tggccggata
     9241 gcccttcgct ctccggccac ttgcaggcta tgatagttct ccagtcacag gagaacgtta
     9301 atgaaaccgc aggtgtacca cgtcgatgct tttacctctc aaccatttcg cggcaattct
     9361 gccggtgttg ttttccccgc cgataatctt agcgaagcac aaatgcagct tatcgcccgc
     9421 gagttaggcc attcggaaac tgcttttctg ctgcacagcg acgacagcga tgtgcgcatc
     9481 cgctacttta cgccaacggt tgaagtgccg atttgcggtc acgcgacagt agctgcgcac
     9541 tatgtacgtg cgaaggtgtt aggtttagga aattgcacga tctggcaaac atcgctggca
     9601 ggaaaacatc gcgtgactat cgaaaagcac aacgatgatt atcgtatttc gctggaacaa
     9661 ggtacgccgg gctttgaact gccgctggag ggtgaagtac gcgcggcgat tatcaacgca
     9721 ttgcatctta ctgaagacga tattctgccg ggtttgccga tccaggtcgc gacgaccggg
     9781 cattctaaag tgatgatccc attgaagccg gaagtggata tcgacgctct ttcgcctgac
     9841 cttaatgcgc tgaccgctat cagtaaacag attggctgca atggtttctt cccgttccag
     9901 atccgtccag gcaaaaacga aaccgatggt cgtatgttct cgcctgcgat tggtattgtg
     9961 gaagatccgg tgactggcaa tgccaatggc ccgatgggtg catggttggt acatcacaac
    10021 gtattgcccc acgatggcaa cgtgttgtgt gttaaaggcc atcaggggcg cgcattgggg
    10081 cgtgacggca tgattgaagt gacggtgaca attcgtgata accaaccgga aaaagtcacc
    10141 atttcaggca cagcggtgat tttgtttcat gccgaatggg caattgaaat ctgaaatcgt
    10201 tgcctgatgc gcttcgcctg tcaggtctac aaccgggcta atgtatgccg gataaggcgt
    10261 ttacgccgca tccggcaacc tgacataaat tattttcccg cctccggatg cgtatcaaac
    10321 gccgccatca ccagcgctaa ctcatccaca gtaaagccat gtttcgcatc atccacgccc
    10381 agaccaaact gttcctgcat cacggcatat aacgacgcca catccggtag atttcgctgc
    10441 tccaccgcgt gcccattttc ataatgggta aaatgaaaat tggtcagtgt cagcttgccg
    10501 ccgtccggca aatggcggca catcagcaaa tgatggcgaa aatgcgactg cggccagtgc
    10561 gccgaccaga aattgcccat cacataatcg ctttgttgct gctcgcagag atcgaagcgg
    10621 tacatcggct gccaatgctg atgatgatta aactgcaaca cccaatcatc accctcctgc
    10681 aacaaccgat actctccgtg tggcgtggtc tgcacgagat cggaaactaa acgaatcggc
    10741 gcggttagcg tctgcccacc gaaaccgaca tcagcaatcc atttttcccc ttccagttcc
    10801 accaacagca aacgatgggt gcgcggcggt aatgctggcg gatttgataa cactacgcgc
    10861 cctaacaagc tgcgaacgtt aaaccccagc tcgcgtaaca cccgctcaaa cacgccattc
    10921 tgctcaaaac agtaaccgcc acgacgggct atcaccagtt tctcttccag cgattgatca
    10981 tcaagctgta tttccctcgg cagcaaaacg tcaaggtttt caaacggaat ggtgcaattg
    11041 tgtttcaggt gcaatgcacg aagcgtatca atattgaccg cagcagctcc cgaccagtta
    11101 atacgggcaa aatagtgatt cagaatgggc gtcatcaggc gtttccctta gtggttttct
    11161 caacttataa cctggaatag acgccgcacc ggctattttg tgcgatcgcg gacatgcatc
    11221 acgtttgcaa atcgccaatc ccgttaattt cgttacttat tgcgttacgg actacgctta
    11281 aaaaaacctt cttcgcgagg cgataccatg agccgattca tccccataga attacaccat
    11341 gccagccgtc tgttaaatca tggcccaact gtcttgatca ccagtttcga tgagcaatcc
    11401 cagcggcgta acattatggc tgcagcctgg tcaatgccgg tggagtttga acctccacgc
    11461 gtggcaattg tggtagataa atcgacatgg accagggagt tgattgaacg taacggtaag
    11521 tttggcatcg ttatcccggg cgttgcagca actaactgga cgtgggcggt gggaagtgtg
    11581 tcggtgcgtg aagaagataa atttaattgc tatggcattc cggtggtgaa aggtccggtg
    11641 cttggtttgc cacttgttga agagaaatgc ctggcgtgga tggagtgccg cctgctacct
    11701 gcgacttctg cgcaacaaaa atacgacacg ctgtttggcg aggtggtatc agcagcggca
    11761 gacgcacggg tatttgtcga aggccgctgg cagtttgatg atgataagct caatacgttg
    11821 catcatttag gtgctggcac gtttgttact agcggcaagc gtgttacggc aggttaagca
    11881 ttacatgctg taaccgggtt tctttatcaa agcctccatt tggggctcga tttcggcatc
    11941 ccagatagct tgccaagatt ctggttgaat ctgctgtaga gcaatgctta tcgagctatc
    12001 tttactgttc agatggcgaa taataacgtc ggtaatatct gcagcaagtg ctgctttttg
    12061 ttgttcgtcc agttcacgcg gaaagcattt aatgtcgatg tgcggcatga ttacgctcct
    12121 tttgaaagat gtcagtaaaa ttaacacaca ttgtgaatgt catgtgtccg gtaaagcgtt
    12181 tatacgacaa taatgcccag ggcaagatta cgaaagcccg ctccccgtaa ggactgacgc
    12241 caggtagttt ctgcccatgg ctgcttttcg catcttacgt cttaaccctg ccttgaatac
    12301 cttatcattc gtcaaaatat taatagcaat gtgccgtatc cctgaaaata attctgctgc
    12361 atttcctctt cttattttgc agtcgtcttc attcattacc acgtccagac gccaatgcag
    12421 cttattctcc acgtgccagt ggtttcggat tgctgtggcg aacttctctg cggttaaatc
    12481 agcagaactg atataatatc tgaccgtcat ttctggctct ttcttttgtt ctgctattat
    12541 ggaccgaaag gagactgcca cgcataattt cttcagccct ttccattcaa acgtgaaatc
    12601 aataagttca tcagggacat cgcaaacaat atgaagacgg atttcttctc tgccgtgact
    12661 cttttcacta attgcgtaac tgtcattctc tggattattt aattctttca gcggaaattt
    12721 ttcctcaaag gctttattta gccgcccctg gtttcctttt accgcgaata aataatcacc
    12781 tccctgtttt tgtatcttct ctgcaatatc tttctggcaa cccatcgcat cagttgtgat
    12841 gatttttcct ttaatatcca gcatgttaag aagttcagga atggctgtaa tctcattgga
    12901 tttctcatcc gtcttaatct gcccgagaat cagactgtgc attgttgaga acgcactaat
    12961 gacatgaatt gctcccctgc ggcactcttg tcataagagt gccggagcgt ttttccatca
    13021 attgcaatga cgtctttatc atctgaagaa tggcagtcac gcatccagtt aataaagcac
    13081 tcgtgaaatt ttgcaggact gatacaggat acaactctgg cgatggtatc gtgaacagga
    13141 ataccatttt caaaatcacc atattgtttt aaaaaatcga gatgtgtttc cccaaaatcc
    13201 tctatatctt cccagccttc tgcaccagaa ataacggcac aaatagtcaa cagtagaata
    13261 tccgataatt tatgttccac tttccaggct tgtttttaat cggggataat agaaatatgt
    13321 tccatcaatt ttttaagttc cattttgttc tccttaatta tgtaaggagt atttgatcat
    13381 gtataagcaa taaaaaacag cttcaggtaa taaggaatat ctcaattttt aaacataaaa
    13441 tgcgaattat ttagtacaaa aagtgaactc ctcagggcaa gattacgaaa gtccgctccc
    13501 cgcaaggact gacgcgagat agtttctgtc catggctgct tttcgcatct tacgtcttaa
    13561 ccctgccttg aataccttat cattcgtcaa aacattaata gcaatgtgcc gtatccctga
    13621 aaataattct gctgcatttc ctcttcttat tttgcagtcg tcttcattca ttaccacgtc
    13681 cagacgccag tgcagattat tctccacgtg ccagtgattt cgaatcgctg tcgcaaactt
    13741 ctcagcggtt aaatcagcag aactgatata atatctgacc atcatttcgg gctctttctt
    13801 ttgctctgct attattgagc gaaaggagac tgccacgcat aattttttca gccctttcca
    13861 ttcaaacgtg aaatcaataa gttcatcagg gacatcgcaa acaatatgaa gacggatttc
    13921 ttctctgccg tgactctttt cactcattgc ggaactgtca tgcgctggat tatttaattc
    13981 tgtcagcgga aattttttct caaaggcttt atttagccgc ccctggtttc cttttaccgc
    14041 gaataaataa tcacctccct gtttttgtat cttctctgca atatctttct ggcaacccat
    14101 cgcatcagtt gtgatgattt ttcctttaat atccagcatg ttaagaagtt ctgggatagc
    14161 tgtaatctca ttagatttct catccgtctt gatctgtccg atgaccagac tgtgcattgt
    14221 tgagaacgca ctaatgacat gaatcgctcc cctgcggcga ctcttgtcat aagagtgccg
    14281 gagcgttttt ccatcaattg caatgacgtc tttatcatct gtagaatggc agtcacgcat
    14341 ccagttaata aagcactcgt gaaattttgc aggactgata caggatacaa ctctggcaat
    14401 ggtatcgtga acaggaatgc cattttcaaa atcaccatat tgcttcaaaa aatcgagatg
    14461 tgtttcccca aaatcctcta tatcttccca accctctgca ccagaaataa cggcacaaat
    14521 agtcaacagt agaatatccg ataatttatg ttccactttc caggcttgtc tgtaatcggg
    14581 gataatagaa atatgttcca tcaatttttt aagttccatt ttgttctcct taattatgta
    14641 agaagtattt gatcatgtat aagcaataaa aaacagcttc aggtaataag gaatatctca
    14701 atttttaaac ataaaatgcg aattatttag tacaaaaagt gaacttctga tgatctttcc
    14761 ctggcctgcc accatctctt cacaagctta tgcttattac atgttacatg caaaacgtcc
    14821 cgttttgcat ctttgtgcct acggcacacc gtgatctttc cctgatgagg gatatattaa
    14881 ctattgaaca ttagcgcgaa ttatttagtg caaaaaatca gcatcacgtg atgcttttcc
    14941 tgctattaat aacctcctct atttaactat atttttcatt gatgcaattc gtagatttga
    15001 agaagttcaa ctgccacttt tttattttta ctatcgcaat ttttatacca tacccatgct
    15061 tccgtatttt ttactatttt taaatcatcc ataataaaat ccccaccaaa aacagtaaaa
    15121 tacaatgttt cctcattaaa aataaaactc actgcccccc gttactttat tcgtacccct
    15181 t
//
LOCUS       DAESHI010000102        15169 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.103, whole genome shotgun sequence.
ACCESSION   DAESHI010000102 DAESHI010000000
VERSION     DAESHI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15169
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.103"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="KW011_003222"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="KW011_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HBH7661489.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="KW011_003223"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="KW011_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HBH7661490.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="KW011_003224"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="KW011_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HBH7661491.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="KW011_003225"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="KW011_003225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HBH7661492.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="KW011_003226"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="KW011_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HBH7661493.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="KW011_003227"
     CDS             complement(8214..9107)
                     /locus_tag="KW011_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HBH7661494.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="KW011_003228"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="KW011_003228"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HBH7661495.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="KW011_003229"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="KW011_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HBH7661496.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /locus_tag="KW011_003230"
     CDS             complement(10238..11470)
                     /locus_tag="KW011_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HBH7661497.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="KW011_003231"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="KW011_003231"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HBH7661498.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="KW011_003232"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="KW011_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HBH7661499.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..>15169)
                     /locus_tag="KW011_003233"
     CDS             complement(14275..>15169)
                     /locus_tag="KW011_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HBH7661500.1"
                     /translation="FSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQV
                     YMLDVGQGLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEH
                     LDHRGGLDSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGN
                     NHSCVVKVDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVSHHGSNTSSSLPLI
                     QRVNGKVALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWRISS
                     LREQILPRWYHQWFGVPVDNG"
BASE COUNT         3560 a         4283 c         3739 g         3587 t
ORIGIN      
        1 aatgtgccac ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactct cctgagaagg cgcttcgtca cttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccagccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccag
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttca gacgtacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ttcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt
      841 ctgttcgcgg gaggttaact cctcggtcag tcggctaagt tcgatttcca tctgctcgat
      901 ggcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcgcgac ggtgtaagcg
     1141 ccgttcaacg acgttatctt tcaccatgcg catcactgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 attgtccatc tccgcttcgc agaaagtcag tgctttttcc agctggttgc ggcgggaccg
     1321 gttattactc agttgcgcat gcagctcgtc acggcgaatt cgcgcccgct cttctgcccc
     1381 gctatcggcg cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgacca cgcaacgctt cacgagcgcg agtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagc ttttcgttga gatcgctatt accgctcagc atttctgccg agtcagaata
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgctgagagt acgcgtaatc ttcttttagc tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ttctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 ggttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcaaat agagatcttc
     2341 cgggcaatcg gtcaagcctt ccaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacgccgcc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacag cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcaccg cactgctcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgttcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gcgcttccag ctcctgatgc agagcttcca gttcatcaat
     2881 atcaaaattc ttgccctgac gtttgcagaa gtctgccagc aggcgttcgg cttcttgctg
     2941 ctcgcgcaga cgctgttcca gttcacttaa gcgcatccgc aacggttgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcag cagttcgcgg gcgacatccc aagcctcgtt
     3061 acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttccag
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcgc gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gctgtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccgacat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgcg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgccc gcatccagca cacgcagttt ttgcggaccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaagag ttaaatccgc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaggcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagtgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacgcc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcaacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccaacg ccgaggtagc
    12721 ttcgtccaga atcagaatcg ggctatcacg caacaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcta tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcgaccgttt tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcggg cacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgattag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg
    13861 tgacaacagc gtacccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctccctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgtt atttgcccct gatgaggagt
    14401 atcaagccac ttataaccct gttgttgata gcgatgttta actttgcttg agggcaatcg
    14461 ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa
    14521 tggcaacgat gatgaggtat tactgccatg gtgagatacc tgaagcaatg ttgtctgcac
    14581 ttgctgccag taacggctta gcatcttttg ttcagcgggg acttcaatat caccggttag
    14641 aagaatgcta ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcctttatc
    14701 gttgctagct tgtaaaggcc agtgcacgct gaaacgcaat ccttgccatt gccacgcttc
    14761 gccacgcaca cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg
    14821 ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat gactcagaat
    14881 aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact
    14941 atccccttcg ggccaggcca gtcctgtgtc atagagaatc gctttgccgt ttctggctat
    15001 caccattgcc agcccttgcc cgacatcaag catgtacacc tgccactcgt caggtcgagg
    15061 tttttgccac agcggccagc acatcagcaa gcctacagcc acacacattg ctggcaacgt
    15121 tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaat
//
LOCUS       DAESHI010000103        15143 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.104, whole genome shotgun sequence.
ACCESSION   DAESHI010000103 DAESHI010000000
VERSION     DAESHI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15143
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.104"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..122
                     /locus_tag="KW011_003234"
     CDS             <1..122
                     /locus_tag="KW011_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBH7661501.1"
                     /translation="GTVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            456..2735
                     /gene="ydeP"
                     /locus_tag="KW011_003235"
     CDS             456..2735
                     /gene="ydeP"
                     /locus_tag="KW011_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HBH7661502.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2983..3180
                     /gene="safA"
                     /locus_tag="KW011_003236"
     CDS             2983..3180
                     /gene="safA"
                     /locus_tag="KW011_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HBH7661503.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            3255..4016
                     /gene="ydeO"
                     /locus_tag="KW011_003237"
     CDS             3255..4016
                     /gene="ydeO"
                     /locus_tag="KW011_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HBH7661504.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMSALAI"
     gene            4417..6099
                     /locus_tag="KW011_003238"
     CDS             4417..6099
                     /locus_tag="KW011_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HBH7661505.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            6151..7308
                     /locus_tag="KW011_003239"
     CDS             6151..7308
                     /locus_tag="KW011_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBH7661506.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(7274..7396)
                     /locus_tag="KW011_003240"
     CDS             complement(7274..7396)
                     /locus_tag="KW011_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661507.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESIINYSPFVLQHP"
     gene            7605..9290
                     /locus_tag="KW011_003241"
     CDS             7605..9290
                     /locus_tag="KW011_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HBH7661508.1"
                     /translation="MITIPITFYMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQTELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
     gene            9328..11700
                     /locus_tag="KW011_003242"
     CDS             9328..11700
                     /locus_tag="KW011_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBH7661509.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            11757..14540
                     /locus_tag="KW011_003243"
     CDS             11757..14540
                     /locus_tag="KW011_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBH7661510.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            14902..>15143
                     /locus_tag="KW011_003244"
     CDS             14902..>15143
                     /locus_tag="KW011_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBH7661511.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT         4446 a         3297 c         3289 g         4111 t
ORIGIN      
        1 tggggacagt aaataaaagc aaagtacctc tgggtttaag cgcaacctat ggacaaacag
       61 gaaacaaggt ttcagccgga accgtccagt cagtcattgg tgtcactttt atctatgaat
      121 aattgtgatt ttgaccttag tctatgcgct ccattcacga aaattgcgca gataattatt
      181 ttgtgaaggc tattatccta cacctgtaag aaaatccgcg cacaattgaa gccagagtct
      241 tattttttat attgaaaaat atgttaattt aaacactatg agagatgatg cttaacttat
      301 cgtatttaat ctattgttta acgataatag attaaaaata gaaagagagt agtcccctac
      361 tcttatatat ccatgttggc gataatccct ttgtatgtac tgtgcatcat cgctattaca
      421 aatcctaata attcatttcc acacaggata agtagatgaa gaaaaaaatt gaatcctacc
      481 agggtgctgc aggtggttgg ggtgctgtta aatccgtagc gaatgcagta cgtaagcaga
      541 tggatatacg ccatgatgtt attgccatgt ttgacatgaa taagccagag ggctttgact
      601 gtccgggttg tgcatggcca gatcctaagc acagtgcgtc attcgacatt tgtgaaaacg
      661 gcgcaaaagc aatcgcctgg gaagtcacgg ataagcaggt aaatgcctct ttctttgctg
      721 agaatacggt tcaatcatta cttacctggg gagatcacga gcttgaggct gcagggcgtc
      781 tcactcagcc tttgaaatat gatgccgtca gcgactgtta caagccatta agctggcaac
      841 aagctttcga cgaaattggc gcacgccttc aaagctatag tgatcccaat caggttgaat
      901 tctatacttc gggccgcact tccaatgaag ctgcctttct ttatcagctt tttgcccgtg
      961 aatacgggag caataacttt cccgactgct ccaacatgtg ccatgaaccg acaagcgtgg
     1021 gtttggcagc gagtatcggt gtaggtaaag ggaccgtgtt gctggaagac tttgagaagt
     1081 gcgatttagt catttgcatt gggcataacc ctggtacaaa ccaccctcgc atgctgactt
     1141 cgttgcgcgc tttagtgaaa cggggagcga aaatgatcgc catcaatcct ctacaggaac
     1201 gtggcctgga gcgatttacc gcaccgcaaa acccgtttga aatgctgacg aactctgaga
     1261 ctcagttggc cagtgcctac tacaacgtgc gcattggtgg cgatatggcg ttgctcaagg
     1321 ggatgatgcg cctgttaatt gagcgcgatg atgctgcaag tgccgcaggt cggccctcat
     1381 tgcttgatga cgaatttatt caaacgcata ccgtcggctt tgacgagcta cgccgtgacg
     1441 ttctcaattc cgagtggaaa gatatcgaac gtatttctgg actaagtcag acacaaatcg
     1501 ccgaactggc tgacgcatat gccgctgccg atcgcaccat tatctgttac ggaatgggga
     1561 tcactcagca cgaacatggt acccagaatg tacagcaact ggtcaatctg ctgttgatga
     1621 aaggtaacat tggcaagcct ggtgcgggta tctgcccact acgaggtcac tctaatgtac
     1681 agggcgaccg aaccgtcggt atcaccgaga aaccgtctgc agagtttctg gctcgcctgg
     1741 gtgagcgcta tggcttcacc ccacctcatg cacctggaca tgctgcaatt gccagcatgc
     1801 aagcaatatg cacggggcag gctcgagcat tgatctgcat ggggggcaac tttgcactgg
     1861 caatgccaga tcgggaagcg agcgctgtac cgttaacgca attagatttg gcggtacacg
     1921 tagccactaa gcttaaccgc tctcatctgc tgaccgcacg gcatagctat attctgccgg
     1981 ttctggggcg tagcgagatt gacatgcaaa aaagcggtgc gcaggcggta accgttgagg
     2041 attcaatgtc gatgattcat gcctcgcgtg gcgtgttaaa acccgccggt gtaatgctga
     2101 aatcagagtg tgcagtggtc gcgggaatcg cgcaggcagc actaccccac agcgtggtag
     2161 cctgggagta tctggtggaa gattatgatc gcattcgcaa tgacattgaa gctgtgttac
     2221 cagagttcgc cgactataac cagcgcatcc gacatcctgg aggttttcac ctgataaatg
     2281 cagctgctga aaggcgctgg atgacgccgt caggtaaggc taatttcatt accagcaaag
     2341 ggctgttaga agatccctct tcagcgttta acagtaagct ggtcatggcg acagtacgta
     2401 gccacgatca gtacaacacg acgatttatg gtatggatga tcgctatcga ggggtattcg
     2461 gtcaacgaga tgtggtcttt atgagtgcta aacaagctaa aatttgccgt gtaaaaaacg
     2521 gcgaaagagt taatcttatt gcgcttacgc cagacggtaa gcgcagctca cgccgcatgg
     2581 atagattaaa agtggtcatt taccctatgg ctgaccgctc actggtgacc tattttccag
     2641 aatcgaatca catgctaact cttgataacc acgatccatt aagtggcatt cctggttata
     2701 aaagtattcc ggttgaatta gaaccatcaa attaattcgc ctcctcattt cttctgctgt
     2761 catccgcaca gcagaagaat ttctcattga ctattatcgc gcaatttgct cacattgatt
     2821 aaattaaacc acacactata agatataaac ctctgcctac agctgtaaga aactccgctc
     2881 agtactgaag tgccagtcct atttcctctt ttctccagcc tgttatatta agcatactga
     2941 ttaacgattt ttaacgttat ccgctaaata aacatatttg aaatgtatgc gaccacagtg
     3001 aaaaacaaaa tcacgcaaag agacaactat aaagaaatca tgtctgtaat tgtggttgtc
     3061 ttattactga cccttacgtt gatagccatt ttttcggcaa ttgatcagct gggtatttca
     3121 gaaatgggtc gcattgcaag agatcttaca catttcatta tcaatagttt gcaagactga
     3181 aaataaacag caaattataa atatgaaatg ttaaaaaagt atcgataaaa actttattgt
     3241 tttaaggaga taaaatgtcg ctcgtttgtt ctgttatatt tattcatcat gccttcaacg
     3301 ctaacatttt agataaagat tacgccttct ctgacggcga gatcctgatg gtagataacg
     3361 ctgttcgtac gcattttgaa ccttatgagc ggcattttaa agagatcgga tttactgaaa
     3421 ataccattaa aaaatatcta caatgcacta acatccagac agtgacggtg cctgttcctg
     3481 cgaagttttt acgtgcttca aatgtaccga ctggattgct taatgaaatg attgcttatc
     3541 ttaactcgga agaacgcaat catcataatt tttcagaact tttgcttttt tcttgcctgt
     3601 ctatttttgc cgcatgcaaa ggtttcatta cactattaac taacggtgtg ctatccgttt
     3661 ccggaaaagt gagaaatatt gtcaacatga agctggcgca cccatggaag ctgaaagata
     3721 tttgtgactg cctgtacatc agtgaaagcc tgttgaagaa aaaactaaag caagagcaaa
     3781 cgacattctc acagattcta ttagatgcca gaatgcagca cgcaaaaaat ttgatacgcg
     3841 tagaaggttc agtcaataaa attgccgaac aatgtggtta tgccagtaca tcttatttta
     3901 tttatgcgtt ccgcaaacat ttcggcaaca gtccgaagag agtttctaag gagtaccgtt
     3961 gtcaaagtca catgggtatg aatacgggca acacgatgag tgctttagct atttgattat
     4021 ttgctaacga gtagtcaacc acgcacgctg cgtaagaatt aaatggggca gccattccct
     4081 gccccgcgtt gtttttaggc gatatattta ttgaaataaa taagtgacat ccatcacata
     4141 tttatgcact tgcataacct gttgcagatt atttatgatc tcaattcggc attttgtcag
     4201 taaaatgcaa taatttatta aatatcaata aattagttgt ttatcggcga gaaattactt
     4261 aatagaacag aaagtaatgt caacgcttta tggactgtta tttccctttt tttagctaaa
     4321 tctgctatct ctttatgtga ctaacttcac ttacatccac ttatttctct tcgtaaaatt
     4381 acttcggaat taagtacaat aagaagagga acatttatga agtctgcatt aaagaaaagt
     4441 gtcgtaagta cctcgatatc tttgatactg gcatctggta tggctgcatt tgctgctcat
     4501 gcggcagatg atgtaaagct gaaagcaacc aaaacaaacg ttgctttctc agattttacg
     4561 ccgactgaat acagtaccaa aggaaagcca aatattatcg tactaaccat ggatgatctt
     4621 ggttatggac aacttccttt tgataaggga tcttttgacc caaaaacaat ggaaaatcgt
     4681 gaagttgtcg atacctacaa aatagggata gataaagcca ttgaagctgc acaaaaatca
     4741 acgccgacgc tcctttcatt aatggatgaa ggcgtacgtt ttactaacgg ctatgtggca
     4801 cacggtgttt ccggcccctc ccgtgccgca ataatgaccg gtcgagctcc cgcccgcttt
     4861 ggtgtctatt ccaataccga tgctcaggat ggtattccgc taacagaaac tttcttgcct
     4921 gaattattcc agaatcatgg ttattacact gcagcagtag gtaaatggca cttgtcaaaa
     4981 atcagtaatg tgccggtacc ggaagataaa caaacgcgtg actatcatga caacttcacc
     5041 acattttctg cggaagaatg gcaacctcaa aaccgtggct ttgattactt tatgggattc
     5101 cacgctgcag gaacggcata ttacaactcc ccttcactgt tcaaaaatcg tgaacgtgtc
     5161 cccgcaaaag gttatatcag cgatcagtta accgatgagg caattggcgt tgttgatcgt
     5221 gccaaaacac ttgaccagcc ttttatgctt tacctggctt ataatgctcc gcacctacca
     5281 aatgataatc ctgcaccgga tcaatatcag aagcaattta ataccggtag tcaaacagca
     5341 gataactact acgcttccgt ttattctgtt gatcagggtg taaaacgcat tctcgaacaa
     5401 ctgaagaaaa acggacagta tgacaataca attattctct ttacctccga taatggtgcg
     5461 gttatcgatg gtcctctgcc gctgaacggg gcgcaaaaag gctataagag tcagacctat
     5521 cctggcggta ctcacacccc aatgtttatg tggtggaaag gaaaacttca acccggtaat
     5581 tatgacaagc tgatttccgc aatggatttc tacccaacag ctcttgatgc agccgatatc
     5641 agcattccaa aagaccttaa gctggatggt gtttccttgc tgccctggtt gcaagataag
     5701 aaacaaggcg cgccacataa aaatctgacc tggataacct cttattctca ctggtttgat
     5761 gaggaaaata ttccattctg ggataattac cacaaatttg ttcgccatca gtcagacgat
     5821 tacccgcata accccaacac tgaggactta agccaattct cttatacggt gagaaataac
     5881 gattattcgc ttgtctatac agtagaaaac aatcagttag gtctctacaa actgacggat
     5941 ctacagcaaa aagataacct tgccgccgcc aatccgcagg tcgttaaaga gatgcaaggc
     6001 gtggtaagag agtttatcga cagcagccag ccaccgctta gcgaggtaaa tcaggagaag
     6061 tttaacaata tcaagaaagc actaagcgaa gcgaaataac taaaccttca tgcggcggat
     6121 ttttccgccg ccttattgag cgagatagcg atgcacgtta cagccaagcc ctccagtttt
     6181 caatgtaatc tcaaatgtga ttactgtttt taccttgaaa aagagtcgca gtttactcat
     6241 gaaaaatgga tggatgacag cactctgaaa gagttcatca aacaatatat cgaagcgtct
     6301 ggcaatcagg tctattttac ctggcaaggc ggtgaaccca ctctggctgg cctggatttt
     6361 ttccgtaaag ttattcacta tcaacaacgc tatgcaggcc aaaaacgtat ttttaatgca
     6421 ttacaaacga atggcatttt attgaataat gaatggtgtg cctttctcaa agaacatgaa
     6481 tttctggttg gtatctcgat cgatggcccc caggagttac atgaccgtta cagacgcagt
     6541 aattcaggta acggtacttt tgcaaaagtg atagcagcca tcgagcgtct gaaatcatat
     6601 caagtagagt ttaatacgtt aaccgtcatt aataacgtta atgtccatta ccctcttgag
     6661 gtttatcatt ttttaaaatc tatcggcagt aaacatatgc aatttatcga attgctagaa
     6721 accgggacgc cgaatattga tttcagtggt catagtgaga acacattccg tatcattgat
     6781 ttttctgtgc ctcccacggc ttatggcaag tttatgtcaa ccatttttat gcaatgggtt
     6841 aaaaacgatg tgggtgaaat tttcattcgt cagtttgaaa gctttgtcag ccgttttttg
     6901 gggaatgggc atacaagttg tattttccag gagtcctgca aggataatct ggttgttgaa
     6961 agtaatggag acatttacga atgcgaccat tttgtctatc cacagtacaa aattggaaac
     7021 attaataaat ctgaactcaa aacgatgaac agtgtacaac tgacagcgca aaaaaaacgg
     7081 atttcagcga aatgtcagca atgtgcatat aaacctatct gcaatggcgg ttgtcctaag
     7141 catcgtatta ctaaagtaaa caatgagact gtttcttatt tttgcgaagg ttataaaatc
     7201 cttttttcaa ccatggtacc ttatatgaac gccatggttg agttagctaa gaacagagta
     7261 ccgctttacc acattatgga tgttgcaaaa caaatggaga ataattaata atgctctctt
     7321 ttaactccat tcttagcctc cttcttatga ccttaacaag gcacccgaca taaattcctg
     7381 tcgggtgttt cgtcatcatc atcttccctt gcgaaaatcc tggcttacgt ggtatgcgtc
     7441 agggcattag caataccctt gattatcaat agcataacca taaccataac cactgaaaat
     7501 taaaaatttc tatatatttc atattgttaa atgatactta aataaatttc atcagtaaat
     7561 cattgccata gataatcatt ggatggcaca atcaattttc gctaatgata accattccca
     7621 ttacgtttta tatgttgata gcaaagtatc tctgtctgct gaagcccttt tggctgcgta
     7681 agaataataa aacttcagtg ttgttaatca ttattatcct cgcaatgatc cttggcgtgg
     7741 ttaaaattca ggtctggtta aacgactgga ataatgattt tttcaatgcg ctaagccaga
     7801 aagaaaccga taagctttgg cagcttgttc tttggttccc tgccctgctg gggatctttg
     7861 tattaatatc cgtaaacaaa acatggttaa ttaaactact gactattcgc tggcgcgaat
     7921 ggttaacaga ttactacctt aatcgctggt ttgcagataa aaactactac ttcacgcaga
     7981 tctacggcga gcataaaaat acagataacc ctgaccaacg tatcgctgaa gacattctct
     8041 tactgatcag caaaacgctc agtctttcat ttggcttcat ccagtcactt agcatgctga
     8101 tcacctttac cgttattctt tggcaaagcg cgggtacgct ctcattcact gttggtggaa
     8161 cagaatggaa tatccagggg tatatggtct ataccgtcgt gctcattgtg atcggtggaa
     8221 ctttatttac ccataaggtg ggtaaacgta ttcgtccgct taatgtggaa aaacaacgta
     8281 gcgaagcgac atttcgaact aatcttgtgc agcataataa gcagacagaa ctgattgcgc
     8341 taagcaatgc tgagtctcta caacgccagg agttgcgcga caattttcat accattaaag
     8401 agaactggca tcgtttaatg aatcgtcagc ggtggcttga ttactggcaa aatatttatt
     8461 cgcgctcgct tagcgttctt ccctactttc tgttattgcc gcagtttatt agcgggcaga
     8521 tcaatctggg cggactgatg aaatcgcgcc aggcatttat gctagtatcg aacaatttaa
     8581 gctggtttat ttataaatat gacgaacttg ctgaactggc tgcggttatc gatcgcttgt
     8641 atgagttcca tcaactcact gaacagcgcc ctacgaataa gcctaaaaat tgccaacatg
     8701 cggtacaagt ggctaatgcg agtattcgta cgcctgataa taagatcata ttagagaact
     8761 tgaactttca tgtttcgcca ggcaaatggc tattactgaa aggctactct ggtgcgggaa
     8821 aaaccacact gcttaaaaca ttatcccact gctggccgtg gtttaaaggt gatatttctt
     8881 ctcctgctga cagttggtat gtgtcacaaa caccgttaat caaaagcggc ttactgaaag
     8941 agattatttg taaagcactt cccctgcccg tagacgataa atcgttgagc gaagtactgc
     9001 atcaggttgg tctggggaaa ttggctgcgc gtattcatga ccacgatcgc tggggagata
     9061 ttctttccag cggcgaaaaa caacgtatcg ccctggcacg attaatttta cgacgtccga
     9121 aatggatatt tcttgacgaa actacctctc atcttgagga acaagaggct atacgcttac
     9181 tgcgtttagt gcgtgaaaaa ctacccacaa gcggcgtcat tatggttaca catcaacccg
     9241 gtgtctggaa cctggctgat gatatttgtg acattagcac ggttttataa caacaataaa
     9301 agccctgctg tacggagata taaataaatg aagcgagttc ttattcctgg cgtcatttta
     9361 tgtggcgctg atgtggcgca ggccgtcgat gacaaaaaca tgtacatgca tttttttgaa
     9421 gagatgacgg tctatgctcc tgtccctgta cccgtaaacg gcaacacgca ttacaccagt
     9481 gaaagcatcg agcgtttacc gaccgggaat ggcaatatca gcgatctgct gagaaccaac
     9541 cctgcggtac gcatggattc aacgcaaagt acctcgttga accagggaga tattcgccct
     9601 gagaaaatct ctattcacgg tgcgtcgccc taccagaatg cctatttgat tgacggtatt
     9661 agtgcaacta ataacctgaa cccagcgaat gagtccgatg ccagtagtgc aaccaatatt
     9721 agcgggatgt cacaggggta ttatcttgat gtcagcttac tggacaatgt gacgctttat
     9781 gacagttttg tgccggttga atttggtcgc ttcaatggcg gggtaattga tgcaaagatc
     9841 aaacgcttca acgctgatga tagcaaggtg aaattgggtt atcgcactac gcgttcggac
     9901 tggttaacat cgcatatcga tgagaataac aagagcgcat ttaatcaagg ctcttcagga
     9961 agtacttatt actccccaga ttttaaaaag aacttttata ccttgtcgtt taatcaggaa
    10021 ctcgctgata acttcggcgt taccgccggt ttatcgcgcc gccagtctga tatcacccgc
    10081 gcggattatg tttcgaatga cggcattgtc accggtcggg cacagtataa aaacgttatc
    10141 gatactgcat tgagcaaatt tacctggttt gccagcgacc gctttaccca cgatttaacc
    10201 ttaaaatata ccggctccag ccgtgattat aataccagca ccttcccgca gtctgatcgc
    10261 gaaatgggta ataaatccta tggtctggca tgggatatgg atacgcaact cgcatgggcc
    10321 aaactacgta ccaccgttgg ttgggatcat attagtgatt atacccgtca cgatcatgac
    10381 atctggtaca ccgaactttc atgtacatat ggtgatatta cagggcgttg tacctgtggc
    10441 ggattaggac acatttccca ggctgtagat aattacacct tcaaaacacg cctggactgg
    10501 caaaaattcg ccgtgggtga tgtttcgcat caaccctact tcggcgcgga atacatctat
    10561 tccgatgcat ggactgaacg ccataaccag tctgaatcct atgtgattaa tgctgcagga
    10621 aagaaaacta accataccat ttaccataaa ggtaaaggca gcctgggaat tgacaactac
    10681 acgctgtata tggcggagca cattagctgg cggaatgtgt cgttaatgcc cggtgtgcgt
    10741 tatgactatg acaactatct gtcaaaccac aatatctccc cgcgctttat gacggaatgg
    10801 gatatttttg ctgatcaaac ctcaatgatt accgccggtt ataaccgtta ctatggcggg
    10861 aatattcttg atatgggatt acgtgatatc cgcaatagct ggacggaatc ggtatcaggt
    10921 aataaaactc tgacgcgtta tcaggatttg aaaacgcctt ataacgatga actggcaatg
    10981 ggattgcagc agaaaatcgg taagaacgtt attgcgcgtg caaactatgt ttaccgtgaa
    11041 gcgcatgatc aaatcagcaa aagcagtcgt accgacagcg cgactaaaac caccattact
    11101 gaatataaca acgatggcaa aaccaaaacg cattcgttca gcctcagttt tgaactggcc
    11161 gaacccctgc atatccgcca agtagatatt aacccacaaa ttgtctttag ctatatcaag
    11221 agcaagggca acttgtcgtt aaacaatggt tatgaggaga gcaataccgg tgataaccag
    11281 gtggtttata acggtaatct ggtctcttac gatagtgttc cagtggcaga ttttaataac
    11341 ccattaaaga tctccttaaa catggatttc acgcatcaac cgagcgggtt agtgtgggcg
    11401 aatacgctgg cctggcaaga agcgcgtaaa gctcgcatta tccttggtaa gacaaatgcg
    11461 caatacatca gcgaatattc agattacaag cagtatgttg acgaaaaact ggatagcagc
    11521 ctgacctggg acacccgctt gtcctggacg ccacaatttc tgaaacaaca aaacctgacg
    11581 atcagtgccg atattctcaa tgtactggat agcaaaaccg ctgttgatac aacgaacacc
    11641 ggtgtggcga cctacgccag tggccgtact ttctggcttg atgtcagcat gaaattttaa
    11701 tagttcggat tttacttaac cgggtaaatt cgcccggttt tcgcatggag ataattatga
    11761 gaaacctctg tttcttactg acgttagtgg caactctgtt gctccccggg cggctgattg
    11821 ccgccgcctt accgcaggat gaaaagttaa ttaccgggca actggacaat ggcttgcgat
    11881 atatgattta tccgcatgct caaccaaagg atcaggtaaa tttatggctg caaattcata
    11941 ccggttcatt gcaggaagaa gacaatgagc gcggcgtggc tcattttgta gaacatatga
    12001 tgtttaacgg cacaaaaaca tggccgggta ataaagtcat cgaaacattt gagtcaatgg
    12061 gcctgcgttt cggtcgcgat gttaatgcct ataccagcta tgacgaaacg ctgtatcagg
    12121 tgagtctgcc gactacgcag aaacaaaatc tgcaacaagt gatggcaatc ttcagtgaat
    12181 ggagtaatgc cgcaaccttt gaaatactcg aagtagacgc tgaacgcggc gtaattactg
    12241 aggaatggcg tgcccatcag gatgcgaaat ggcgcacctc tcaggcgcgc cgccctttcc
    12301 tgctggcaaa tacccgtaat ttagaccgtg aacctatcgg cctgatggat actgtcgcca
    12361 cggtcacacc ggcacaattg cgccaatttt atcaacgctg gtatcaacca aataatatga
    12421 cctttatcgt ggtcggcgat atcgacagta aagaagcgct ggcgctgata aaggataatt
    12481 taagtaagct tccggctaac aaagcagctg aaaatcgcgt ctggccgaca aaagccgaaa
    12541 accacctgcg ctttaatatc attaatgata aagaaaaccg agtgaacggc atcgcactct
    12601 attatcgcct gccaatggta caagtgaacg atgagcaaag ctttgtcgaa caagctgaat
    12661 ggagcatgtt agttcagctg ttcaatcaac gtctgcagga acgcatacag tcgggcgagt
    12721 tgaagactat ttctggcggc actgcgcgca gcgttaaaat tgcacccgat tatcagtcgc
    12781 tgtttttccg tgtaaatgca cgagacgata atatgcagga tgctgcaaat gcattaatgg
    12841 cagagttggc aaccattgat cagcatggtt tttctgctga agaactcgat gatgtcaaat
    12901 ctacccgcct cacctggctg aaaaatgcgg ttgatcagca agctgaacgt gatttacgta
    12961 tgctgaccag tcgcctggca tccagctcat taaataatac gccgttcttg tcgccggaag
    13021 agacatatca actttcgaaa cgtctgtggc agcaaattac cgtgcaaagt ctggcggaaa
    13081 aatggcagca gttaagaaag aaccaggacg cattttggga gcaaatggta aacaatgagc
    13141 ttgccgccaa aaaagcattg tctcttgcgg ctatcctggc gctggaaaaa gagtacgcca
    13201 acaaaaagct ggcggcttac atcttcccag gcagaaattt atcgttaaca gtagacgctg
    13261 acccacaggc ggaaattagc agcaaagaaa cgctggctga gaatctgaca tcattaacac
    13321 tttccaatgg tgccagggtt attctggcaa aatccgcggg tgaagagcaa aagctacaaa
    13381 ttactgccgt atctaataaa ggcgatttaa gtttccctgc gcagcaaaaa tcacttatcg
    13441 cgctggcaaa taaagcagta agcggaagcg gcgttggtga gctctcctct tccagcctga
    13501 aacgctggag tgcggaaaat tcggtaacca tgagcagtaa agtcagtggc atgaatacgt
    13561 tgctctccgt tagcgcgcgg actaataacc ctgaacctgg ttttcagttg attaaccagc
    13621 gaatcaccca cagcacgatt aacgacaata tttgggcatc gctacaaaat gctcaaattc
    13681 aggcgttgaa aacgctcgac cagcgtccag cggagaaatt cgcccagcag atgtatgaga
    13741 cgcgctatgc tgatgaccgc acgaaattac tgcaagaaaa tcagattgta cagtttactg
    13801 ccgcagatgc gctggctgcc gatcgccagt tgttttcatc tccagcggat atcacgtttg
    13861 tcattgtcgg taatgtctca gaagacaaac tcgtggcgtt aattacgcgt tacttaggat
    13921 caatcaaaca ctctgattcg ccattagccg caggtaaacc attaactcgc gcgacggaca
    13981 acgcatcggt tactgtaaaa gaacaaaatg aacctgtggc acaggtttca cagtggaagc
    14041 gttatgattc ccggacacct gttaatctgg cgacgcgtat ggcgctcgat gcttttaacg
    14101 tcgcactggc aaaagatcta cgcgttaata ttcgtgaaca ggcatctgga gcatacagcg
    14161 tttcttctcg cctctcggtt gatcctcagg ccaaagatat cagtcatttg ctggctttta
    14221 cttgtcaacc agaacgacat gatgaactgt taacgttagc gaatgaagtg atggttaagc
    14281 gtctggctaa agggatcagt gagcaagaac tgaatgaata ccagcaaaac gttcagcgca
    14341 gcctcgatat ccaacagcgt agcgttcaac aattagcgaa cactattgta aatagtctta
    14401 ttcaatatga cgatcctgca gcatggactg agcaggagca attgttgaaa caaatgacgg
    14461 tagagaatgt taacactgcc gttaaacaat atctttctca tccggtaaat acttatactg
    14521 gagtattatt gccaaaataa taacagcccc gtcaacacat cgttggcggg gattttagca
    14581 atattcgcca tttttatgta ataattttat aaatgcgttc aaaataataa ccaggtacta
    14641 atagtgatat tttaaggtct gatttttacg tgaaaattca ggaggcacag aatgcgcata
    14701 aaaataacag cataaaacac cttaccacca cccaagaatt tcatattata ttgtttttca
    14761 atgaaaaaat attattctcg taatatctca cgataaataa cattaggatt ttgttattta
    14821 aacacgagtc ctttgcactt gcttacttta tcgataaatc ctactttttt aatgcgatcc
    14881 aatcatttta aggagtttaa aatggataag aagcaagtaa cggatttaag gtcggaacta
    14941 ctcgattcac gttttggtgc gaagtctatt tccactatcg cagaatcaaa acgttttccg
    15001 ctgcacgaaa tgcgcgacga tgtcgcattc cagattatca atgacgaatt atatcttgat
    15061 ggcaacgctc gtcagaacct ggccactttc tgccagacct gggacgacga aaatgtccac
    15121 aaattgatgg atttatccat taa
//
LOCUS       DAESHI010000104        15030 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.105, whole genome shotgun sequence.
ACCESSION   DAESHI010000104 DAESHI010000000
VERSION     DAESHI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15030
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.105"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(54..2006)
                     /gene="betT"
                     /locus_tag="KW011_003245"
                     /pseudo
     CDS             complement(54..2006)
                     /gene="betT"
                     /locus_tag="KW011_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
     gene            2145..2732
                     /gene="betI"
                     /locus_tag="KW011_003246"
     CDS             2145..2732
                     /gene="betI"
                     /locus_tag="KW011_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HBH7661512.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVAGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2746..4218
                     /gene="betB"
                     /locus_tag="KW011_003247"
     CDS             2746..4218
                     /gene="betB"
                     /locus_tag="KW011_003247"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HBH7661513.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4232..5902
                     /gene="betA"
                     /locus_tag="KW011_003248"
     CDS             4232..5902
                     /gene="betA"
                     /locus_tag="KW011_003248"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HBH7661514.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPTLDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            6115..6783
                     /locus_tag="KW011_003249"
     CDS             6115..6783
                     /locus_tag="KW011_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661515.1"
                     /translation="MSEQIKQDIDLIEILFYLKKKIRVILFIIAICMAMVLLFLYVNK
                     DNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQIPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(7026..7721)
                     /locus_tag="KW011_003250"
     CDS             complement(7026..7721)
                     /locus_tag="KW011_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HBH7661516.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(7714..9141)
                     /locus_tag="KW011_003251"
     CDS             complement(7714..9141)
                     /locus_tag="KW011_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HBH7661517.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(9152..9871)
                     /locus_tag="KW011_003252"
     CDS             complement(9152..9871)
                     /locus_tag="KW011_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HBH7661518.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(10399..11253)
                     /gene="rclR"
                     /locus_tag="KW011_003253"
     CDS             complement(10399..11253)
                     /gene="rclR"
                     /locus_tag="KW011_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HBH7661519.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            11479..12804
                     /locus_tag="KW011_003254"
     CDS             11479..12804
                     /locus_tag="KW011_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HBH7661520.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            12913..13149
                     /locus_tag="KW011_003255"
     CDS             12913..13149
                     /locus_tag="KW011_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBH7661521.1"
                     /translation="MFKKSVLFATLLSGVMEFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            13161..13754
                     /gene="rclC"
                     /locus_tag="KW011_003256"
     CDS             13161..13754
                     /gene="rclC"
                     /locus_tag="KW011_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HBH7661522.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(13914..14783)
                     /locus_tag="KW011_003257"
     CDS             complement(13914..14783)
                     /locus_tag="KW011_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBH7661523.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
BASE COUNT         3938 a         3828 c         3594 g         3670 t
ORIGIN      
        1 gtcaatgatt cccgcgccat atgcagacat ggcgcggttt tatgcaaaaa caatcacgcg
       61 tccgggaaca tcacgctatg gcccggcgct tcacgatgga gatgaataaa gttaaggtgc
      121 cgctcgtact ggtcaagaat atcggtgatc acctgctctt tgctgtagtc catcaggtcg
      181 ttgccctggc tgccttctaa caggaaggtt tccagtcggt agtaggtcga tttaccgata
      241 cgtgcgcggt aggtaaagcc cggcaccgaa tattgctgcg gccaaatctg atagacaaag
      301 ttttgctctt cgcccatatg caccaacaaa tccagatgcc ccaactgttg tccctcttcc
      361 ggcggcaggc tttttagctc cacgtacgcg ccgcgcaacc gcagctcctg cgccacttct
      421 tccattgccg ggtaacagac cgtctccatc atctgtttag tgtaacgcgt gcccggataa
      481 ttcatcaggc gcgatagacg ttttttccag ctcaggcgat cctgaagccc cagcggtcgc
      541 ggcgcggtat cgcggttgac actttcacgg cggtaatctt ctaccttcag agatttatac
      601 aaccccgcca tcacgaagaa aatcacaaag ctgaacggca gccccatgat taccgtggtg
      661 ttttgcagcg cggatatccc gttggtcatc agcatgccga gcgtcagcag accaatcgcc
      721 accgaccaga agacgcgcag ccagccgggg gcgtcgctgt tgatatcttt aagctgcgag
      781 gtgaaattcc ccagcaccag cgcaccggag tccgccgagg tcacataaaa cagcaggcca
      841 gtaatggtgg cgacggaggc gctaaaggta aacgccggat actgcgccag caggctgtag
      901 aagccgcgct ccggatggac catcgcttcc tcggcaaatg ccgcgccgcc gtggatgatt
      961 tcatacagcg cgctattgcc gaataccgag agccataaca gcgtgaaggt aaacggaata
     1021 atcaacgtgc ccagcacgaa ctggcgaatg gtacgcccac gcgagatacg cgccaggaac
     1081 aagccgacaa acggcgacca tgccacccac catgcccaga agaagagcgt ccagttattc
     1141 atccactcaa ccggacggtc gaaggcaaaa ctgttgagcg tcatgcccat aaagcgattc
     1201 acatagtcgc caacgttcag caccagcgca ttaagcagga acgaggtgtc gcccataaac
     1261 aatacgaaca ggatcaatcc cagcgccagc gcaacgttaa gttccgataa cacacgaatg
     1321 cccttatcga caccggaggt gacagagatc gtggcgatta tcaccgacaa ggcgatcagt
     1381 gccgcttttg ccgccatcga atcgggaata tcaaacagta cgctcaagcc atagttaagc
     1441 tgcaccacac cgataccgag cgtagtggca ataccgaaga tagtgccgat caccgctgca
     1501 atatccactg agtgacctat cggcccgtta atccgtttac cgaagatcgg gtacagcgcc
     1561 gagcggatgg tgagcggcaa attataacga tagctaaagt atccgagcgc catgcccatc
     1621 agcgcataca tcgaccagcc ggttaagccg tagtgaaaca gcgtccagac catcgcctga
     1681 cgcgcggcct caatcgtctg tcccgcgcct tccggcggct gcatatactg cgttaccggt
     1741 tcggctacgg agaagaacat caggtcgata ccgatcccgc cagcaaacag catcgccgcc
     1801 caactcagca ggctgaattc cggtttggat tgttctggcc cgagcttcac cgaaccaaaa
     1861 cgcgaacaag cgatacagac cacaaagaca atatagagcg ttgccgccag tagatagtac
     1921 caaccgaagg ttttagaaac ccagtccagc gtgcggccaa tccacagggc cgagaagtcg
     1981 cgaaacagga tcgttgtcag ggaaaaggtc tgtcatccag tatccccact gttattgtta
     2041 ccatttaaaa tcaaattcgt aacaattacg acacatttta tattgaacgt ccaatcaata
     2101 accgctttaa tagataaaca acgctgatga atggagtggc gaaaatgccc aaattgggga
     2161 tgcagtcgat ccggcgcaga caactgatcg acgccacact ggaagcaata aatgaagtgg
     2221 gcatgcacga tgcaacgatc gcgcagatcg cccgccgtgc aggcgtttct acggggatca
     2281 tcagccacta tttcagggac aaaaatggtc tgctggaagc aaccatgcgc gatatcacca
     2341 gtcagctgcg tgacgcggtt ttgaatcgat tacatgcact tccgcagggc agtgcagagc
     2401 agcgattaca ggcgattgtt gccggaaact tcgatgaaac tcaggtgagc agtgcggcga
     2461 tgaaagcctg gctggcgttc tgggccagca gtatgcatca gccgatgctc tatcgtttac
     2521 agcaggtcag cagccgccgc ttgctgtcga atctggtgag cgagtttcgc cgcgaattgc
     2581 cgcgcgaaca ggcacaggaa gcgggctacg gccttgccgc gctgattgac ggattatggc
     2641 tgcgcgcggc actaagcggc aaaccgctgg ataaacctct cgcacactcg ctgacccgtc
     2701 actttattac tcagcattta cccaccgatt aaccgaggag acgtgatgtc ccgaatggca
     2761 gaacagcagc tttatataca tggtggttat acctccgcca ccagcggtcg caccttcgag
     2821 accattaacc cggccaacgg taacgtgctg gcgaccgtgc aggccgccgg gcgtgaggat
     2881 gtcgatcgcg ccgtgaagag tgcccaacag gggcaaaaaa tctgggcggc gatgaccgct
     2941 atggagcgct cgcgtattct gcgtcgggcc gttgatattc tgcgtgaacg caatgacgaa
     3001 ctcgcaaaac tggaaaccct cgataccgga aaagcatatt cggaaacctc aaccgtcgat
     3061 atcgttaccg gtgcggacgt gctggagtac tacgccgggc tgatcccggc gctggaaggc
     3121 agccagatcc cgttgcgtga gacgtcattt gtttataccc gccgcgaacc gctgggtgtg
     3181 gtggcgggga ttggcgcatg gaactacccg attcagattg ccctgtggaa atccgccccg
     3241 gcgctggcgg caggcaacgc aatgattttc aaaccgagcg aagtcacccc gcttaccgcg
     3301 ttaaagctgg ctgaaattta cagcgaagcg ggcctgccgg acggcgtatt taacgtgttg
     3361 ccgggggtgg gcgcggagac cgggcaatat ctgaccgagc atccgggcat tgccaaagtg
     3421 tcatttaccg gcggtgtcgc cagcggcaaa aaagtgatgg ctaactcggc ggcctcttcc
     3481 ctgaaagaag tgaccatgga actgggcggt aaatcaccgc tgatcgtttt cgatgatgcg
     3541 gatctcgatc tcgccgccga tatcgccatg atggcaaact tcttcagctc cggtcaggtg
     3601 tgtaccaatg gcacccgcgt cttcgttccg gcgaaatgca aagccgcatt tgagcagaaa
     3661 attctggcgc gcgttgagcg cattcgcgcg ggcgacgttt tcgatccgca aactaacttc
     3721 ggcccgctgg tcagcttccc gcatcgcgat aacgtgctgc gctatatcgc caaaggcaaa
     3781 gaggaaggcg cgcgcgtact gtgcggcggc gatgtactga aaggcgatgg cttcgataac
     3841 ggcgcatggg ttgcaccgac cgtgttcacc gattgccgcg acgaaatgac catcgtgcgc
     3901 gaagagatct tcggtccggt gatgtcgctt ctcacttatg aatctgagga cgaagtgatt
     3961 cgccgcgcca atgataccga ctacggcctg gcggcgggca tcgtgacggc ggacctgaac
     4021 cgcgcgcatc gcgtcattca tcagctggaa gcgggtattt gctggatcaa cacctggggc
     4081 gaatccccgg cagagatgcc cgttggcggc tacaaacact ccggcattgg tcgcgagaac
     4141 ggcgtgatga cgctccagag ttacacccag gtgaagtcca tccaggttga gatggctaaa
     4201 ttccagtcca tattctaacc aggaggttta tttgcaattt gactacatca ttattggtgc
     4261 cggctcagcc ggcaacgttc tcgctacccg tctgactgaa gatccgaata cctccgtgct
     4321 gctgcttgaa gcgggcggcc cggactatcg ctttgacttc cgcacccaga tgcccgcagc
     4381 gctggcgttc ccactacagg gcaaacgcta caactgggcc tatgaaacag agcctgaacc
     4441 gtttatgaat aaccgccgca tggagtgcgg acgcggtaaa ggcctgggtg gatcgtcgct
     4501 gatcaacggc atgtgctaca tccgtggcaa tgcgctggat ctcgataact gggcgcaaga
     4561 acccggtctg gagaactgga gttatctcga ctgcctgccc tactaccgca aggccgagac
     4621 tcgcgatgtg ggcgagaacg actaccacgg tggcgatggc ccggtgagcg tcactacctc
     4681 caaacccggc gtcaatccgc tgtttgaagc gatgattgaa gcgggcgtgc aggcgggcta
     4741 cccgcgcacg gacgatctca acggttatca gcaggaaggt tttggtccga tggatcgcac
     4801 cgtcacgccg cagggccgtc gcgccagtac cgcgcgcggt tatctcgatc aggccaaatc
     4861 gcgccctaac ctgaccattc gtactcacgc tatgaccgat cacatcattt ttgacggcaa
     4921 acgcgcggtg ggcgtcgaat ggctggaagg cgacagcacc atcccaaccc gcgcaacggc
     4981 caacaaagaa gtgctgttat gtgcaggcgc gattgcctca ccgcagatcc tgcaacgctc
     5041 cggcgtcggc aacgctgaac tgctggcgga gtttgatatt ccgctggtgc atgaattacc
     5101 cggcgtcggc gaaaatcttc aggatcatct ggagatgtat ctgcaatatg agtgcaaaga
     5161 accggtttcc ctctaccctg ccctgcagtg gtggaaccag ccgaaaatcg gtgcggagtg
     5221 gctgtttggc ggcactggcg ttggtgccag caaccacttt gaagcaggcg gatttattcg
     5281 cagccgagag gaatttgcgt ggccgaatat tcagtaccat ttcctgccag tagcgattaa
     5341 ctataacggc tcgaatgcgg tgaaggagca cggtttccag tgccacgtcg gctcaatgcg
     5401 ctcgccaagc cgtgggcatg tgcggattaa atcccgcgac ccgcaccagc atccggcaat
     5461 tctgtttaac tacatgtcac acgagcagga ctggcaggag ttccgcgacg caattcgcat
     5521 cacccgggag atcatgcatc aaccgacgct ggatcagtat cgtggccgcg aaatcagccc
     5581 cggcacggaa tgtcagacgg atgagcagct cgatgagttc gtgcgtaatc acgccgaaac
     5641 cgccttccat ccgtgcggta cctgcaaaat gggttacgac gagatgtccg tggttgacgg
     5701 cgaaggccgc gtgcatgggc tggaaggcct gcgcgtggtg gatgcgtcaa ttatgccgca
     5761 gattatcacc gggaatttga acgccacgac aattatgatt ggcgagaaaa tagcggatat
     5821 gattcgtgga caggaagcgc tgccgaggag cacggcggga tattttgtgg caaatgggat
     5881 gccggtgaga gcgaaaaaat gagtcgtgat gtgaactaac gcaggaaccg atcagattga
     5941 gagttaccgt tccagagagg gggaccgaat ccttatataa acactgaggt aactctcatg
     6001 cttcatatcc agtattcatg atgctggttt tttgctgata tttaccccga acattcctga
     6061 tacagactat taatacaacc ttatctgcgg cagattttat tttctggata tattatgagt
     6121 gaacaaatca aacaggatat cgatctgatt gagattttat tttatctgaa gaaaaagatt
     6181 cgtgttatcc tttttattat agctatctgt atggcaatgg tgcttttatt tctgtatgtc
     6241 aataaagata atataaaagt gagttacagc ctaaaaataa accagacaac accaggcata
     6301 cttgttaact gtgatagcaa taataacttt gcctgtcaga ccacaatgac tgaagatgtt
     6361 atacagcgaa ttactacatt ttttcacacc agcccagatg tcaagaacag agaaataaag
     6421 ctggaatggt caggagataa gagagattta ccaactgctg aagcggaaat atctcgcgtg
     6481 caggcgtcta ttatcaaatg gtatgcgtca gaataccata atggcaggca agttctcgat
     6541 gagatacaaa taccttcagc aattaacagt gagctttata caaaaatgat atacctgacc
     6601 aggaactggt cattgtatcc gaacggtgat ggctgtgtaa ctataagctc accagaaata
     6661 aaaaataaat accctgctgc catttgcctg gctctgggat tttttctaag cattgtaatt
     6721 tctgtaatgt tttgccttgt caaaaaaatg gtagatgaat accaacaaaa ctctgggcag
     6781 taataaacag cattctgata gagagtgcct gtataaagct gaagtgacaa tgaccgggaa
     6841 gggaacccgt tttcaggaat ggatgcctgt ctttttcact gtaaatatcc tggcattact
     6901 aatgccagga tatttacaga agagcagcat aaccatgacg gaacggggtt aagtcaggtt
     6961 gtccttaacg ctattttcaa cctcgtatgc cttcttcagg tttatgttca gatctcatat
     7021 ctctctcagc aatcctcaat aatcagatac accgctttca ccggaccatg aaccccgacg
     7081 actttgataa gctcaatatc cgccgttgag ctgggaccac tgatgatgtt aatacatgac
     7141 ggcattcgtt caccggcctg cgctttctga tgcaattttt ctgcgagttg cgctacacgc
     7201 ggcaggattg tgcttttacg caggataaaa agagaagatt ccgggagcag gctcaatgaa
     7261 cgcccgcgct cgacggcgga aaaaagaacc acgcctcccg attcggttaa accatattca
     7321 gcatacacga cacccacttt agcctgctct gcctgcgaga tattctcggc acctttcgcc
     7381 ggatcccaaa caacggcatt gcattcctgc tgcaaacgtt cactaatccc caattcctcc
     7441 agcctcgtgt caccgctaat cacgaccgac tgatctccca gctctttaca cagacgtatt
     7501 gcagcttctg ccgccttcgc ctcgctggtc agctcacagc gcgtcaacat aacatcgctg
     7561 gcaaactgaa taaacgcgtc acagcgctgc tgttggttaa gttgggtaag ccgctcgtta
     7621 gcatagttgt taagcggcgc atcttctgct tgcggttcaa gtcgcagcgg gcgacccagt
     7681 gcctgagcaa cgttattcaa aaattcgctc cgattatcca ttctttttct cctgcgcctg
     7741 atgtttctta aaccaactac ggaaactctc tccgtcagct tcaggaagat cgcgtgcttc
     7801 catccagtcg ctaatcgcgc caaatttgag tggtgttttg ccgccattga taaaccagct
     7861 tgccgcatga gcgccggcca tcatcccgac tttccacaat cctggatgac tattggcata
     7921 agcgaacatt tttatcgccc gttgctctgc ttttgcggtg atcccttttt cagccatcac
     7981 ccgacgatga cgcaaaatca gttttgacag cggaatacgc accggacaca cgctgtcaca
     8041 agcagtgcat aaagagcagg cgtagggtaa atctttaaaa tctttatagc cgccaagtag
     8101 cggagaaatc accgcaccaa ttggccctgg ataaatagag ccatatccat gaccgccaat
     8161 atggcgatat gccggacaag tattcataca agccccgcag cgaatacagc gcagcacatc
     8221 ccgaaattca gaggccagca cctcagaacg cccgttatcg acaataacca gatgaaactc
     8281 ttcaggacca tcaacgtgcc ccgcttcgcg cggtcctgtc agccaggtgt tgtatcccgt
     8341 caaacgtgca ccaacggcac tgcgcgccag catggtgatc aatacatcta cctcggcaaa
     8401 cgtgggggca atacgctcca ttcccatcac tgcaatatgc gttttaggca gcgtggtaca
     8461 cattcgcgca ttaccttcat tggtcactag gcataccgaa cctgtctctg ccaccgcgaa
     8521 attacagccg gtaataccta tttcagcact gaggaaatct tcgcggattt tttgccggat
     8581 gaataaggtc atcgcttcag gcgtttccgg cccctcatag cccagacgtt cgtgtagcac
     8641 tcgacggatc tgatggcgat ctttatgaat tgccgggacc acaacatgag atggcggatc
     8701 ttgatccagc tggagaatat attcacccag atcggtttca atcacctgaa tgccagcatc
     8761 ctgcaacaca tgattgacac caatctcttc ggtcaccatc gatttagatt tcaccacctt
     8821 ccgggcattt ttgcgttggg caacctgtaa aatgtagcgg gtagcgtctt ctttggtttt
     8881 tgcaaaccag acgtgaccgc cgttttgcgt cactttttct gagagctggt acagataagc
     8941 gtcgagatta ctcagaacat gatcacgtat ctgggcggcc cgatcgcgcc actcctccca
     9001 gtgccccaat tcatcgacca ttttttgccg atttgcccca atacgctgct gcgcgtttgc
     9061 caccgctttg cgcatgatcg gatcttcaat ttgctgacgg atgcgtgtct taaaatctgt
     9121 attactggtt ttgatcgaca tctttatatc ctcagcggct catcaacact tcagcaatat
     9181 gcatcacttt gactttctgc ccttcccgtt gtaatcgccc actgatgttc agcaggcaac
     9241 tcacgtcagc accaattaaa tactcagggc ggacttccat caggtgcaca accttttctt
     9301 tcaccatctc gccggatatt tcggccattt tgaccgagaa cgtgccgcca aatccgcagc
     9361 aggtatcctg ttcagcaaag gtcaacagct ccagtccacg cacatttttc agcagcgtaa
     9421 gtggctcgtc cttcactccc agcttacggg ccaggctaca agatgggtga tacacagctc
     9481 tcccttgcaa actggcacct acatcgacta cccctaattt attaacaata aaagaggtga
     9541 gatcctgcat acgcgcggca accttttcgg cacgtgatgc ccattcgggt tcatccgcca
     9601 gatacgtcgg gtaacttttt acggcatagg tgcaagagcc agccggtgaa ataatgggat
     9661 cgtcgttatc ctccagtgcg gcgatcagat ttttcatccc tggaatcgct tctttgatat
     9721 aaccgctatt gatcgcaggc tgaccgcagc atccctgttt ctccgggaaa tttacgcgac
     9781 agccgagttt ttccagtagc agcacggagt ctcgtgccat tcttgatttc agggcgtcac
     9841 caatacaggt gacaaagaaa ttgacattca caactaatac tccattactt catgcccatt
     9901 tatgcccgat ggttcccctc cttccggggg atcaatcatc aataaatatg gcgttctttt
     9961 ttaattggct agcaacatac cacccctcat ttgtgtgaca aatgataatc tttcatccaa
    10021 acttcaaatg caataactaa ttttatttct ttcggcatcg ctcacatttt atggggaaat
    10081 cgacgttcat ttttggcata agaaatccat ttaaaaaggc tacatccctt atacctattc
    10141 agagctactt attcaggggt tattgagata tacactgatg gtgtacaaaa aaatcaccga
    10201 caataatcac gtatcaagta atagtcgggg ttttatttaa ttgaagcaat ttagtttgca
    10261 tttttattta aaaactgagc tagagtccat cagaaaaaaa tatataaagc ccaaaccacc
    10321 gttggaaata gaaatagttg aaaggctcac aactcccgga atgcggatta tctttgccct
    10381 gatttctggc ggttttattc agggtgcaag ctgtctgacc ctttcccgat attctcccgg
    10441 agtacaacca aactcgcgga caaacgcctt gtgaaaagat gattcactgg catagcctac
    10501 tgactcagcg atcaccacaa cagggagcat ttcccgggaa aacatctggg ccgctatttg
    10561 tagacgcaac tttgttaata cagccagcgg cgtggttccg gaaacatcac ggaaaagctg
    10621 ggcaaaactt gcccgagaca tgtgggcgat gctggccagc gattcgacgg tccaggcgtg
    10681 tcctggcatt tccagcattt gctgtattac cgcaccaaga cgtggatgca gaagcaaact
    10741 gagaatgttt ttctctgtat taacctgtgc aatccactca cgcaccgcga gggtaaagaa
    10801 tgtagcgcag atctggctac acagtgcatc cactcccggc atcgccgatc tggattcctg
    10861 ttgtaaaaac gggatcgcct ccctcagcca gttatattcc acgctgtggt taaccggtgc
    10921 taaaaacagc gtttccggca aagacgttaa aaaataacgc gccgaatgtt gcaaccgaag
    10981 agtgccgcag acaatacagg tcgattcatt atcgacatga ctcaggcgat gagctgaatt
    11041 ttgtggtagc aggaccacat ttcccgggcg taatgtaaaa atctctcccg tcggcatttc
    11101 cagcttcgcc gctccttgcg ttaacgcatg ccaacgaata accgataatt ccccggcacc
    11161 atgttgaagc tgccagtcac ttcctaacac gcaattctta tcgatcgttc cttgtggagc
    11221 gttaagcatc aacaaacggc taagggcatc catatcagac tcctgagtgg tttttgatta
    11281 caaacaccaa caatcttagt aaataactaa tctaatacca tcaaatggtt aatcatgatt
    11341 tttgttttgt gattctgatt aagaaaaaga aaataccaga cgataagaca aaatatcaag
    11401 cgttgtgaag aaatggtatt tgctcttttg cgtctatagt catgatgtca aatgaacgtg
    11461 tttcgacagg aaatcatcat gaataaatat caggcagtta ttattggttt tggcaaggct
    11521 ggaaaaacat tagccgtcac gctggcaaaa gcaggttggc gagtggctct catcgaacaa
    11581 tcaaatgcaa tgtatggcgg gacctgtatt aatatcggct gcatcccaac caaaacattg
    11641 gttcatgacg cacagcagca cacagatttt gtccgtgcca tacagcgtaa aaatgaagtg
    11701 gttaattttt tacgtaataa gaattttcat aatcttgcgg atatgcccaa tatcgacgtg
    11761 atcgacggcc aggcggagtt tatcaataat catagcctgc gtgttcatcg gcctgaggga
    11821 aatctggaaa ttcatggcga gaaaattttt attaataccg gtgcacaaac cgtggttccg
    11881 ccaattcctg gaattaccac cacgcctggt gtatatgaca gcaccggatt acttaatcta
    11941 aaagaattgc ctgggcattt aggtattttg ggcggcggat atattggcgt tgagttcgcc
    12001 tctatgttcg ctaattttgg cagcaaggta accattttag aagcagcttc gctgtttttg
    12061 cctcgggaag atcgggatat tgctgataat atcgcgacga ttttacgcga tcagggcgtc
    12121 gatattatcc tcaatgccca tgtggagcga atcagtcacc atgaaaatca agtgcaagtg
    12181 catagcgagc acgcccaact ggcggtggat gcactgttaa tagcttccgg tcgtcaaccg
    12241 gctaccgctt cgttacatcc agaaaatgcc ggtatcgcag taaacgagcg cggggcaatt
    12301 gtcgttgaca agcaattaca taccaccgca gacaatattt gggcgatggg agatgttacc
    12361 ggcgggctgc aatttactta tatttcactg gatgattacc gcattgtgcg tgatgagttg
    12421 ctgggagaag gcagacgtag tactgatgat cggaaaaatg tgccttattc cgtattcatg
    12481 acaccgccac tgtccagggt tggtatgaca gaagaacagg ccagagagag tggtgctgat
    12541 attcaggtgg tgacattgcc tgtagctgca attccgcgtg ccagagtgat gaatgatact
    12601 cgtggggtat taaaagcgat tgttgataat aaaacccaac gtatattagg ggcatcactg
    12661 ctgtgtgttg actcccacga gatgatcaat atagtgaaaa tggtgatgga tgccgggctg
    12721 ccttatagca tattacgcga tcagatattt actcatccgt cgatgagcga atcactcaat
    12781 gatctatttt cattagtcaa ataaactcaa aatcagacgc cagaacaaat attctggcgt
    12841 ctcagagaaa agaatcttat taattctctg ttactctata acttcttaat cacttcattg
    12901 atggtatttt atatgtttaa aaaatctgtt ttatttgcaa cactattatc tggcgttatg
    12961 gaattttcca ccaatgcaga tgataaaata attctgaaac atatcagcgt ctcgtcagta
    13021 tcagcatcac cgacagttct ggaggatacc attgctgata tagccagaaa atataatgct
    13081 tcatcctgga aagtcacatc gatgcgaatt gataataatt caaccgcaac agcagtattg
    13141 tataaataag gatgttcaca atggaaaaat acctgcacct gttaagtcgg ggagataaaa
    13201 ttggcctgtc attgattcgt ctgagtattg cgattgtttt tatgtggatt gggttattaa
    13261 agtttgtccc ttacgaggca gacagcatta caccattcgt cgcaaacagt ccactaatgt
    13321 cgttctttta tgaacacccg gaagactata aacagtatct gactcacgaa ggcgaataca
    13381 aaccagaagc aagggcatgg caatcggcca ataataccta tggtttttcc aacggtcttg
    13441 gcgtcgtgga ggtgattatt gctctgctgg ttttggctaa tcctgtcaat cgctggttag
    13501 gtttattggg aggactgatg gcatatacca cgccgctggt aacactctca tttttaatca
    13561 ccaccccgga ggcatgggta cccgcattgg gtgacgctca tcatggtttc ccttatttat
    13621 ccggtgctgg tcgcttggta ttgaaagata ctctgatgct ggcaggtgct gtcatgataa
    13681 tggcagattc ggcgcgggaa attcttaaac aacgcagtaa tgaatccagt tcaacgttaa
    13741 aaactgaata ttgataatcc gaacactgtc tgttgttcac tttttcaggc gtcaggcccc
    13801 ctttctgatg atgaagcctg acgccatatt accggagata tgacagagtg aagatcaata
    13861 tcactcccct gttaaaagat tcttgtcgtc tcaggtaaaa tatatgatta atatcaatca
    13921 tgtattttta cagcaagaca accacgaaca aattcaggat taaaatgttt taccgactcc
    13981 ccaacataac ccaaatcaag cgaactaatt tgtgccattt ctttctctgt cagtgagaaa
    14041 tcccagatag caaaattttc ttcgatacgc tgttgatgtg tcgatttcgg aataacgacc
    14101 actccccgtt gaatattcca acgcagaatg acttgagaaa tcgatttttg atgggcatct
    14161 gcaatactct gaagcagatt attttcaaag ggtttatgtc gtccaccacc taacggagcc
    14221 catgcttcag gctgcacgtt ataatacttc atggttgcca gcgcctctgg ttgggcaaaa
    14281 tagggatgca actcgacctg gttgaccatc ggtttaactc ttaccgtttc acaaaagttc
    14341 gccagaacat gaggatagaa attggaaacc ccaattgctt ttaatttgcc ctcttcatag
    14401 gcatcttcga gtgcacgcca cacactgaaa taatcgcgca tagcctggtg caataaatag
    14461 aggtcgaagt actctagtcc cgattttttt aatgatgctt caatacctgc tgctgccgta
    14521 tcctgattca acatatcctg cccccacaat ttagacgtta taaataactc atctcgggta
    14581 cataaacctt cagatatagc ttcacgaacg gcttcaccga cggcatcttc attaccgtat
    14641 accgctgcag tgtcgatgag tcgataaccc gtgcggatag cgttcaacac tgactgtttg
    14701 cacacgtttt tatcggtaac ctgaaaaaca ccaaatccca tcatcggcat tttcagattg
    14761 ttacttaata cggaaaattc cactgtcagt ccctccatta ggcatgaaca atgagtctac
    14821 gttaaaacat aacctcaaat tagtatgtgg attttgatat cacttatgca aaaaattcat
    14881 taataatgta ggactgaaac ctctctattt tcggggacaa cgaagcagac gctaccagtg
    14941 cttttgcctt cgaccttgct gtttttgata cacttagggc cctgggcata acgaaaatgt
    15001 gcgatatcac gaagaattaa tggagaatgt
//
LOCUS       DAESHI010000105        14996 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.106, whole genome shotgun sequence.
ACCESSION   DAESHI010000105 DAESHI010000000
VERSION     DAESHI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14996
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.106"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..296)
                     /locus_tag="KW011_003258"
     CDS             complement(<1..296)
                     /locus_tag="KW011_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH7661524.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFADEAVSGSVK
                     ASLRNGFSSLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREG"
     gene            883..1230
                     /locus_tag="KW011_003259"
     CDS             883..1230
                     /locus_tag="KW011_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBH7661525.1"
                     /translation="MILDSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFI
                     GSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEI
                     GAVPAIPQQSSLF"
     gene            1283..1678
                     /locus_tag="KW011_003260"
     CDS             1283..1678
                     /locus_tag="KW011_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HBH7661526.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            1719..2462
                     /gene="cmoA"
                     /locus_tag="KW011_003261"
     CDS             1719..2462
                     /gene="cmoA"
                     /locus_tag="KW011_003261"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HBH7661527.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            2459..3430
                     /gene="cmoB"
                     /locus_tag="KW011_003262"
     CDS             2459..3430
                     /gene="cmoB"
                     /locus_tag="KW011_003262"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HBH7661528.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(3595..6024)
                     /gene="torZ"
                     /locus_tag="KW011_003263"
     CDS             complement(3595..6024)
                     /gene="torZ"
                     /locus_tag="KW011_003263"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HBH7661529.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKDGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTTRIQHPMVRKSYLDNPLQPT
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIDFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLRGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(6049..7149)
                     /locus_tag="KW011_003264"
     CDS             complement(6049..7149)
                     /locus_tag="KW011_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HBH7661530.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(7537..8283)
                     /gene="cutC"
                     /locus_tag="KW011_003265"
     CDS             complement(7537..8283)
                     /gene="cutC"
                     /locus_tag="KW011_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBH7661531.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(8297..8863)
                     /locus_tag="KW011_003266"
     CDS             complement(8297..8863)
                     /locus_tag="KW011_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH7661532.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            9079..10815
                     /gene="argS"
                     /locus_tag="KW011_003267"
     CDS             9079..10815
                     /gene="argS"
                     /locus_tag="KW011_003267"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBH7661533.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            10992..11480
                     /locus_tag="KW011_003268"
     CDS             10992..11480
                     /locus_tag="KW011_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HBH7661534.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIENDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(11600..11992)
                     /gene="flhe"
                     /locus_tag="KW011_003269"
     CDS             complement(11600..11992)
                     /gene="flhe"
                     /locus_tag="KW011_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HBH7661535.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(11992..14070)
                     /gene="flhA"
                     /locus_tag="KW011_003270"
     CDS             complement(11992..14070)
                     /gene="flhA"
                     /locus_tag="KW011_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBH7661536.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(14063..>14996)
                     /gene="flhB"
                     /locus_tag="KW011_003271"
     CDS             complement(14063..>14996)
                     /gene="flhB"
                     /locus_tag="KW011_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HBH7661537.1"
                     /translation="DPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFS
                     GKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFYLWHHWPQMMRLM
                     AESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQ
                     SEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVA
                     KGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQ
                     LKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
BASE COUNT         3693 a         3939 c         3563 g         3801 t
ORIGIN      
        1 ccttctctaa ggctgataac tgtcacacct ttagcctgta gcgctttaac cgttgataac
       61 acatcaattg tgtcacgtcc aagacggtct acagctacca caactacggt gtcaccttct
      121 cgtgcataag ctaacaaact gctgaaaccg tttcttaaag aagctttgac agaaccactg
      181 acagcttcat cagcgaacca tttagcaatg ttgtaccctg cctcttcaat ttgcatttgc
      241 tggttagtga tggattggtc atctgtactc accctgcaat atccaagtat cgccataatg
      301 ttccccttag aagtctcaaa agttagtctc ataagtttag ctgtctcata atccaaaaac
      361 aaccttttga gactacttga gggtgcattc taccgttacg ttgaaagtgt ctcaaaagga
      421 cttccttatg agactggtta atagagcgct atgaagattc aggagggtaa ctataaccat
      481 cactttctaa ctttcacgta ggaaatcgtt aagtttcttg atagttacac catgtgacca
      541 actatctata tatcgttata tagtcctgta tatagcacta tatgtagtgt ctggagaggg
      601 acgactaaca cgatacagtt taccctgaaa agagacttca cagattcttt atatatttca
      661 ataagttatg ctaaaaagag actataccga tactgcatag aatttgtgta ggattgcata
      721 gatacttgga aaaacaccgt taaattgcat agtcattcac ctgatattgt cctgttttta
      781 caggtagttg attcggatgt tctaggaatc atcctcatag ttgattcggt tagttgggct
      841 tgtaccaacc cctaatcaac ctcatagtgt atttagacct tgatgatttt agacagccgc
      901 cctgttcatg cagcacgtcc acacagtgaa gctattcgcg acgctcaacg aaaaaaacct
      961 aaagttccgg tacatgctgt actgacggcg acaaatccgc tgattcgttt tatcggtagt
     1021 gatgatatga cgcaaaaccg ggaattattt caggtctggt tacagaaatt agcgcagtgg
     1081 catcagacca ctacgcctta tcttttttta catacgccag acattgccca ggccccggaa
     1141 ctggtacata ccctgtggga agacttacgt aaaacgcttc cagagatcgg agcagttccg
     1201 gctattccac agcaatcttc tcttttctga atttgccacc tatcatagac aggtgccatc
     1261 ggccatttta aagggagttt gtatggtaag cgcgctatat gccgttttaa gtgcgttgtt
     1321 attaatgaag ttctcttttg atgtcgttcg cctgcgaatg cagtaccgcg ttgcctatgg
     1381 tgacggtggt tttagcgaac tgcaaagcgc tattcgcatt catggtaacg cggtggaata
     1441 tattcccatc gcgattgtgc tgatgctgtt tatggaaatg aatggcgcag aaacctggat
     1501 ggtgcatatt tgcggcatcg ttttgcttgc tggtcgtctg atgcattatt acggttttca
     1561 tcaccgtctg tttcgctggc gacgttccgg catgagcgcc acctggtgtg cgctgttgct
     1621 gatggtgctg gcgaatcttt ggtatatgcc ctgggagttg gttttctccc tgcgttagcg
     1681 cacaatacgc cactttcttt ttcccggatt tttacgttat gtctcaccgc gacacgctat
     1741 tttctgcccc tatcgccaga ctgggcgact ggacctttga tgaacgggta gctgaagtct
     1801 tcccggatat gatccagcgt tccgttcccg gctattccaa tattatttcc atgattggta
     1861 tgttagccga gcgcttcgtt caacctggta cgcaggttta cgatctgggt tgttctctgg
     1921 gcgcggcgac gctctcggtg cgtcgcaaca ttcatcatga taattgcaaa attattgcca
     1981 tcgacaactc cccggcgatg attgaacgct gcagtcgtca tattgacgcc tataaagccc
     2041 ctacgccagt agacgtcatt gaaggtgata ttcgcgatat cgccattgaa aacgcatcga
     2101 tggtggtgct gaattttacc ctgcaattcc tggaaccttc cgagcgccag gcattactgg
     2161 ataaaattta tcaaggactg aacccgggcg gcgcgctggt gctttcggaa aaattcagtt
     2221 tcgaagatgc caaagttggt gaactgttgt tcaacatgca ccacgacttt aaacgtgcca
     2281 acggttacag cgaactggag atcagccaga agcgcagcat gctggaaaac gtgatgctga
     2341 ccgattccgt ggaaacccat aaagcacgcc tgcataaagc cggttttgag catagcgagc
     2401 tgtggttcca gtgctttaac tttggttcac tggtggcatt aaaagcagag gacgctgcat
     2461 gatcgacttt ggtaactttt attctctgat tgccaaaaat catctttcac actggctcga
     2521 aacgctgccc gcgcagattg ctaactggca gcgcgagcag cagcacgggc tgtttaagca
     2581 gtggtccaac gcggtggaat ttctgcctga aattaaaccg tatcgtctgg atttattgca
     2641 tagcgtaacc gccgaaagcg aagagccact gagcaccggg caaattaaac gcattgaaac
     2701 gcttatgcgg aacctgatgc catggcgcaa agggccgttc tcactgtatg gcgtcaacat
     2761 cgataccgaa tggcgttccg actggaaatg ggatcgtgtt ctgccccatc tttctgattt
     2821 aaccggacgc accattctgg atgtcggctg cggcagcggt tatcacatgt ggcgcatgat
     2881 tggcgcaggc gcgcacctcg cggtggggat cgaccctacg caactgtttc tgtgccagtt
     2941 tgaagcggtg cgtaaactac tgggtaacga tcagcgcgca catttgttac cgttaggtat
     3001 tgaacagctt ccggcactga aagcctttga taccgtcttt tcgatgggcg tgctttacca
     3061 tcgccgttcg ccgctggagc atctctggca gttgaaagat caactggtga atgagggtga
     3121 actggtgctg gaaacgctgg ttattgatgg cgatgagaac acggtgctgg taccaggcga
     3181 tcgttacgcg caaatgcgta atgtctattt catcccttcc gcgctggcgc tgaaaaactg
     3241 gctgaagaag tgtggttttg tagatatccg cattgtggat gtgtgcgtta ccactactga
     3301 agagcagcga cgcactgaat ggatggttac cgaatctctg tccgatttcc tcgacccaca
     3361 tgatccgagt aagacagttg aaggttatcc tgcgcctaaa cgcgcggtgc tgattgcgcg
     3421 caagccgtaa aggtctggta ttactgccgg atgcggcgtg aatgccttat ccggcctaca
     3481 aacccttgct aattcaatct attgcaggga ctatgtaggc ctgataagca tagcgcatca
     3541 ggcagtttta cgtttgcata acctcagcgc ccgtttccgg gcgctattca cttcttacgc
     3601 ctgaacagca ggcttatcaa atgccgttaa cttcggtgcg ttacccgtat atttttcgat
     3661 atacaccagc gcagagttac cggcacaggc atttgccagc tgcgagctgg ggatatccgc
     3721 cgttaacacg ttcgcactgc cgtttttaca caagccgttt tccagatctg gccatgcacc
     3781 ttcatgcacg cataccacgc cttttttgat cccgtcagtc accaccgcgc ctgtcaaaat
     3841 ctgaccacgt ttgttccaga cacgcaccag atcgccattc gcaataccaa atcgagcagc
     3901 atcttcggtg tgaatagtga tcggttcacg atcggcaacc gcatattttt tacgcagttc
     3961 cgcatagtta agctgactgt gtaaacggtg tgccggatgt gcggtcagaa gctgcaactg
     4021 cttctcgtcg gcggtaccct tccactcatc aggcgcaagc caggttgggt gtgccgggca
     4081 atccttatag ccaaattttt ccagcgtttt ggagtaaatc tcaattttgc cacttggcgt
     4141 acccagcgca tttttcaccg gatcggcgcg gaaatcacca taacgaacgt actgttcgtt
     4201 cttctcgctg cggcgcattt cgatcagttt attttgctgc cagaaggcat taaacattgg
     4261 catagtgacg cgttgcgcac gggccccttt ctgagcggca tcatagaaaa atttcagcca
     4321 cgccatttca tctttacctt cggtatagat ctcttttccg ccaggtttga gtaattccgc
     4381 aagatcggca aacacatcaa aatcgtttcg cgcttcaaat tgcggagcaa cagcctgctt
     4441 catcggcaca atatgctggt tgctgtaatc accggtcatc gtcaagtcat tgcgctcaaa
     4501 cgatgtggtg atcggtaata cgatatctgc gtgtttagca gctgcggtcc agtagcattc
     4561 agaaacgacg atcatctccg gtttctgcca tgctttaatc agacgattag tatcctggtg
     4621 atgggtaaag ttaccgccac ccgcccacca gatcattttg atattcggat acgtttgctc
     4681 tttaccgtta tgctgatatt tgccgccagg attttccagc gcatcgacga tacgggcaac
     4741 aggaatcgcc ctcattccgc catcatcagc ggcctcgctg gcctgtccgg caatcgccgc
     4801 agacatttcc ggcagcacgc cgccaacacg tgtcgggttg ccaccattgg agtaatgata
     4861 agagaaacca aaaccgccgc ccggtgtacc aatctgccct aacatcgccg ccagcgtcac
     4921 cagcatccag tgtttctgtt caccatattg ctggcgctga attccccagc ctgccatcag
     4981 cattgtgcga ttcgccgcca tcagttcagc gagtttgact atctgcgctt ccggtacgcc
     5041 agtaatttcc gccgcccagg ccgcgctctt cggcgtatta tcgctcttac ctctcagata
     5101 ctcttcaaac tgcggatacc cggtagtgta tttttcaagg aacactttat cgtgtttgcc
     5161 ttgtgtcatc agggtatgcg caatccctaa catcagtgcc acgtcggtgc ccatattcgg
     5221 cgcgatccag gtggcattat cgccaaagaa gtcgatggtt tcggagcgga taggatcaat
     5281 ggcaatcact ggtttgccag attttttcag ctgatggaag tattccagcc cttgctcatc
     5341 ggtactgctc caggcaattt ttaaggtatt cagcgggttc atcccccaca gcaccacaac
     5401 ctggctgttt tccagaatca gcggccagga ggtctgctgt tcatacacct ctacagaacc
     5461 gaccacatgc ggcatgatca cctgtgccgc cccggttgaa taatcgccgc tatgcccgga
     5521 ataaccgccc gccaggttca tataacgttg cagtaaggtt tgcgctttat gcaacacgcc
     5581 agaagagcgc cagccgtatg atccggcaaa aatagccgat ggtccgttag ctttgcgaat
     5641 gcgatcgtgt tgctcatgaa tcagttttaa tgcttgttcc cagctcacct gtacataggt
     5701 atcttcgcca cgacctttcg tcggttgcag tggattatcg agatagcttt ttctcaccat
     5761 cggatgctga atacgcgtcg tggtgtgtac ctgatccgcc gccgtagact gtaaggaatt
     5821 cggtatggtt ttcgccagcg cgcctgttga agaaacaatc ttgccgtcct tcacttctac
     5881 gttcatcgct ccccaacgtc ccgcggtgag gattttaccg tccttctctt ctgcccatgc
     5941 ggggagcggt gctgccgatg tcaccaccag cgctccagcg gcaataccgc tgtgtttaat
     6001 aaattcacgt cttgttaatg tcataacttc ctccctgatc aacgaggatc actgtttctc
     6061 ggtaatatct ttggcgttgt actggaaata ccgcgttaaa atgtccagtt cgttttcgct
     6121 catgctggtt cgtgccccca ttcctttggc aatggacggc cacgcattga cggtgtaatg
     6181 gtcggcggca ataggggcat gacaaccagc gcaataggta tcgtcaagtt tttcagcgta
     6241 ttgccatagc ggtttacggt ccgctaatcc gggatcggta agcgcaccct gtaaagacgc
     6301 ctgacgccat tgattgccgt attcgtcagc ctgccattcc ccgtttaccg tgagcgcctt
     6361 gataccttct tcacttaatg tggctagcgc cagccgttga cctgccgcca ggtagagcgt
     6421 gttttcactg ccctgcattt gataaccctg caacagaacg atcggctgtt tgccactggc
     6481 atcaacgacg gtgagatcgg taccaggatt cacggtagcc agctcgccta tgcgagaagt
     6541 tttgaaagga taaatatgtg cgccattagt cactgaagta gcggcctgac tttccagctc
     6601 atgcgccgcg ttgtcatcca tttttatttc tggcggaaaa tgggcaatgc ctttatgaca
     6661 atcgatacag gtttcgccgc ccttttgtgc tttgttatgc attttctgcg cagattcact
     6721 ttgcgaggca atatccatgg catcaaaaga atggcaacta cggcacgttg cagagtcagt
     6781 tgcttttaat tctttccata ctgtttcggc catttcctgg cgatgagttt cgaacttatc
     6841 gtcactgtct attttgccgc taacaaattc atgataaata tctttagatg cttttaattt
     6901 agcaaataaa taatccatcc ctgactttgg aatatggcaa tctgcacatt ccgcacgtat
     6961 ccctttctgg ttcgaaaagt ggacagttcc ctgatattcc tcaaaaggtt tactcatcga
     7021 gtggcaggaa aggcaaaatg ctgtatccga cgttttatgt aaggcttttt gcgccagcaa
     7081 taacccgccg ccaccaacca caaccgctat cagcaaaaac aataacccaa tgcgtttttt
     7141 ccctcgcata gattcctcta aataaattcc agaacaccga aatcattgta taattaaata
     7201 cacaacgaat taaccgcttg cataactcgg cacaacactg cctgaaataa tcgataaaga
     7261 atatgattta ttacaatgta atcattaatt gctaaggaat aacccagttg ctatttagaa
     7321 tatatattta tcagttttag caatttaaat cccataatca atgtgaatat aaaaaatatt
     7381 attagcacaa cgctatgttc actgtcaccc gctcaacaga tcacggttat cgttcgtttt
     7441 ttatactgct cagggataaa aaaaggcccc tgttgaaatt gcaggggcct ggtacgagca
     7501 agcatcatat tgggcgacat gatgcaacgg taaaaatcat ttggcctgat ggcgttcaat
     7561 gattcctttc atttcagcaa ccgccgcccc gtctacgata taacgcgaat actcgtccgc
     7621 gtgttcatct gatgacatgg acaatccttg attacgataa cgcatcggtg aggcttgcca
     7681 cgctcccgcg gagctatgga cttccagcac tccggcatcg aggaagtggt gcaggttttc
     7741 tgcacggact cctgctccgg ccataatgat tggagcatca cgatgggcaa taagttccat
     7801 aatttttgat aaaccttgca gtgcgtctga tttttgccct gatgtcagta cccgggcaat
     7861 gcccaattcc gcaagattat tgagtgtata taaagggtta gcgcacatat cgaaggcgcg
     7921 atgaaaagtc actgccagcg gaccggcagc agccattatt ttttccattc gtggcatatc
     7981 gacattcccg tcaacatcga gaacgcccgt caccagtcca ggaaaaccta attcgcggac
     8041 cgtgcgcaca tcctcaagaa tggcggcaaa ctcaccgtcg ctgtagcaaa aatcaccacc
     8101 gcgtgggcga attatcggat gcacagggat cgtcacccgc tggcgcacgg atttcagtac
     8161 ccccaacgac ggcgttaagc ccccctcttt tggggctgcg cataattcaa ctctgtctgc
     8221 gccgttttgc tgcgccgtta gtgcacattc catgctgtaa cagcaaattt ccagtaatgc
     8281 catttttgct ccttaattac gctgactgct cgctggcaac gatctcttca atggaccacg
     8341 gatgaaactt aatagttgtt ttgccatcgg tgaccgccag cgtcggattc ggtaatcgct
     8401 catgctcacc ttttggtgaa ctggttttaa ctgagatccc cggtaggctg agtccttcat
     8461 cagaaagcaa tgccagcgca cgggcattca gcgtttctgg atcacccagc agaacgattt
     8521 caatatgttc ccaaccttcg tgtgggtaac gtttttcccc gggccacggt agctccacaa
     8581 tagaaaactg ccagtgcgca acctgtaccg gttcatgcaa tttaaacaga caaatcggtc
     8641 tgccattgat catattttct gacaaaagct cgccacactg ttcaaacccg cgacgccagc
     8701 gttcagcagt ggcgttttga tggcaacgca aagaaatgtg atcggcagtc agcggagtga
     8761 tattcaaccc cagacggagg gaaagttcat ctaatgcgtg ggtaaatcgc ggtaaatccg
     8821 atgcaatatc ctgcagctcg tcgatagatt gccagttcgc cataatcact cttcgtcttt
     8881 cagtaaaagc gttaatttac cctgttgccc tgcgccaacc aaccgctgat ttcacgccgc
     8941 ttctgatgca atagtgaaaa cggcaatacg ccacgcgcac gttgctgacg aaaacagcca
     9001 tttgcagtat actcccgccc taatttcttt aactggtgcg ggcaattttt gctcgcttca
     9061 tcaatgtaag gtattccggt gaatattcag gctcttctct cagaaaaagt ccgtcaggcc
     9121 atgattgcgg caggcgcgcc tgcggattgc gaaccgcagg ttcgtcattc agcaaaagtt
     9181 cagttcggcg actatcaggc taacggcatg atggcagttg ctaaaaaact gggtatgaca
     9241 ccgcgacaat tagcagagca ggtgctgact catctggatc ttaacggtat cgccagcaaa
     9301 gttgagatcg ccggtccagg ctttatcaac attttccttg atccggcatt cctggctgaa
     9361 catgttcagc aggcgctggc gtccgatcgt ctcggtgttt ctacgccaga aaaacagacc
     9421 attgtggttg actactctgc gccaaacgtg gcgaaagaga tgcatgtcgg tcacctgcgc
     9481 tctaccatta ttggtgacgc agcagtgcgt actctggagt tcctcggtca caaagtgatt
     9541 cgcgcaaacc acgtcggcga ctggggcact cagttcggta tgctgattgc atggctggaa
     9601 aagcagcagc aggaaaacgc cggtgatgaa atggagctgg ctgaccttga aggtttctac
     9661 cgcgatgcga aaaagcatta cgacgaagat gaagagtttg ccgagcgtgc acgtaactac
     9721 gtggtaaaac tgcaaagcgg tgacgaatat ttccgcgaga tgtggcgcaa actggtcgac
     9781 atcaccatga cgcagaacca gatcacctac gatcgtctca acgtgacgct gacccgtgat
     9841 gacgtgatgg gcgaaagcct ctacaaccca atgctgccag gaattgtggc ggatctcaaa
     9901 gccaaaggtc tggcagtaga aagcgaaggg gcgaccgtcg tattccttga tgagtttaaa
     9961 aacaaggaag gcgaaccgat gggcgtgatc attcagaaga aagatggcgg ctatctctac
    10021 accaccactg atatcgcctg tgcgaaatat cgttatgaaa cactgcatgc cgatcgcgtg
    10081 ctgtattaca tcgactcccg tcagcatcaa cacctgatgc aggcatgggc gatcgtccgt
    10141 aaagcaggct atgtaccgga atccgtaccg ctggaacacc acatgttcgg catgatgctg
    10201 ggtaaagacg gcaaaccgtt caaaacccgc gcgggtggta cagtgaaact ggccgatctg
    10261 ctggatgaag ccctggaacg tgcacgccgt ctggtggcag aaaagaaccc ggatatgcca
    10321 gctgacgagc tggaaaaact ggctaacgcg gttggtattg gtgcggtgaa atatgcggat
    10381 ctctccaaaa accgcaccac ggactacatc ttcgactggg acaacatgct ggcgtttgag
    10441 ggtaataccg cgccatacat gcagtatgct tacacgcgtg tattgtccgt gttccgtaag
    10501 gcagaaatta acgaagagca actggctgca gctccggtaa tcatccgtga agatcgtgaa
    10561 gcgcaactgg cagctcgcct gctgcagttt gaagaaaccc tcaccgtggt tgcccgtgaa
    10621 ggcacgccgc atgtgatgtg tgcttacctg tacgatctgg ctggtctgtt ctctggcttc
    10681 tacgagcact gcccgatcct cagcgcagaa aacgaagaag tgcgtaacag ccgtctgaaa
    10741 ctggcacaac tgacagcgaa gacgctaaag ctgggtctgg atacgctggg tattgagaca
    10801 gtagagcgta tgtaatcgat ttttcgtgag agtaaagcct gatcagggtt acctgatcag
    10861 gcttttttat tgccatctaa atgtattctg aaaatggaca tgccattgtt ttctgactgt
    10921 tggataagag gccagaagcg taatatccgg ccccagggaa acgataacgg ttgaatttaa
    10981 ggaataccgc agtgtttaaa tttcttgtat taacattagg cattatctct tgccaggctt
    11041 acgcagaaga tacggttata gaaaacgacc atgacatttc agccatcaaa gattgttggc
    11101 aaaaaaattc agatgatgat actgacatta acgtgatcaa atcatgcctg cgacaagaat
    11161 acaatctcgt cgatgcgcaa ttaaataaag cctatggtga agcttatcgt tatatagaac
    11221 aagtgccacg cacaggtgta aaaaaacctg ataccgaaca acttaacttg cttaaaaaat
    11281 cacagcgagc ctggctggat tttagggaca aagaatgtga attaatcctt tcaaatgagg
    11341 acgttcagga tttaagtgac ccttattctg aatcagaatg gctctcatgt atgatcatac
    11401 agaccaatac gcgaactcgc cagttgcagc tataccgtaa ctctgaagat ttttatccaa
    11461 gccctttgac aagaggataa ttcacatctt tttggcatgt tttgttgcaa gctattcctg
    11521 ataaataatt gcaacaagac atcgagcctt tttcactgag ttattaaaca tactcgcgag
    11581 cgcgtaattt ttttgtcctt cagcgataat tcacaatcac ttcattacgt tgtaccttta
    11641 gcggtggaat taaccgccca ccgcctggca cttcccagat aaatcgcaac ggttctgctg
    11701 ccgctatacc ggaaaaggcc acggtggttc cgctctgccc ctctaattcg acacaacgag
    11761 attgcgaaca caagcgaacc cgcagtcctg aaggtgtcgg gccgataagc tgataacgcc
    11821 acgctaccag cgtcatcaat cctgaagcag gttgtcgcgt agaaagaggc gcagacgaca
    11881 tcgactcacc gcgatgattt agcgtaattc ctacactact tgcctgccac atcccctccc
    11941 cggcggcttg caccagcagc ggaaacaata atattgctaa taaggttctc attatttgcc
    12001 gccaattgtc gccgtcatgc ggatatgtcg gttatcagac agttccagat tcgacaggac
    12061 cactaactgc ggcaagctgc ggcgcaggaa gcgagacaat aatggtcgca gcgcgtggtt
    12121 caccaacaat actggcggcg cgcccagcat ctcctgacgg gatagcgctt cctgagtttg
    12181 cgccagtaaa cgatccgcca gccctggctc cagtcctccc ccgccctgca gcgcctgtag
    12241 taacaaacgt tccagcggtg tatcgaggcc aataacatgg acttcatctt tgccaggaaa
    12301 ccactgctgg gtaatcgccc gtcccaacgc cacgcgcacg acggcggtta attcatgtgg
    12361 atcgctttgg atgggcgcat gttccgccag cgtttcgaga atggtgcgca tatcgcgaat
    12421 cggtactttt tcatcgagga gattttgcag cactttatgc agtgtggtga gcgtgacgac
    12481 gccaggaacg agatcttccg tcagctttgg catctcctgg gcgacgcgat ccaacagctg
    12541 ttgcgcctcc tgacgaccaa acagctctgc ggcatgctgg ctaatgaggt ggttaagatg
    12601 cgttgctacc accgtgctgg cctcaaccac tgtgtacccc tgaatctgcg cctgttcttt
    12661 tagcgcactt tcaatccaga tagcattcag gccaaatgcc ggatcgacgg tcgcctcacc
    12721 aggtaacgtc ccggcagcgg ttccagggtt aatcgccagc cagcgccccg gataagcatc
    12781 accactgcca atctccacgc ctttcatcaa aatgcgatag cgggcaggtt gcagatccat
    12841 attgtcgcga atgtgcacca ctggcggcag aaatcccatc tcctgggcaa atttcttgcg
    12901 gatactgcgt atacggccca acaactcacc atcctgctgg aaatcgacca tcgggatcag
    12961 tcgataaccc acttccattc ccagagaatc ttccagttgt acatcgttcc acgtcgcttc
    13021 gacaacggta ttattctctg ccatttttac cggtttgggt tcggcaggcg ctttttgttc
    13081 gcgtccgcgt atccaccagg ccagcccgag caatccggca gtgaacagca aaaataccag
    13141 gttcggcatt ccaggcacca ggccgagtaa accgagcacg gcggcgctta acaacataac
    13201 gcttgggtta ctgaaaagct gattcaccat ctgctcgcca acatcctgat cggtgctgac
    13261 acgcgtaacg atgacccccg cggcggtaga aatcaccagc gccggaattt gtgccaccag
    13321 accgtcgcca atggtcaata gcgtataact ttccgccgcg tgtcccatgc tcatgccatg
    13381 ttgcagcacg ccgaccagca acccgccgac aacgttaatg accatgatga ggatcccggc
    13441 gatggcatcg ccgcgaacaa acttacttgc cccgtccatt gagccgtaaa aatcggcttc
    13501 ctgagtcact tcggagcggc gttttttcgc ctcatcttca ccaatcaatc cagcgttaag
    13561 gtcggcgtca atcgccatct gcttacccgg cataccatcg agaacaaagc gcgcacccac
    13621 ttctgcgata cgcccggcac ctttggtaat gaccataaag ttgatgatca cgagaatgac
    13681 aaacaccacg ataccgatag cgaaattgcc accaacgagg aagtgaccga acgcttcgac
    13741 caccttccct gccgccgccg cgccggtatg cccttccatc aaaatgatac gggttgaagc
    13801 cacgttaagt gccagacgca acaacgtagt aaacaacaga atggtcggaa acgcagcaaa
    13861 ctcaagcgtg cgctgggtaa acatcgccac cagcaacacc atgatcgaca aggcaatatt
    13921 gaaggtaaac aacaggtcga gtatgaatgc gggcagtggc agcaccatca tcgacaagat
    13981 caacaggatc aaaatcggtc cggcaaggat ctgccattgt gtcgatttca ggtttgcggg
    14041 caggcgcagc atcgcggcca gattactcat gggtcggttt ctcgttaata aaatccaggg
    14101 cttccggcac cggaagatga gtaggttgta cagggcgctg tccaccagcc agacgccagc
    14161 gtttcagttg ccagacccag gccagcactt ccgccaccgc ggcgtacagt tgacccggga
    14221 tttgttgacc aatctccgca tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg
    14281 tcgggacgtt attttcagcg ccaatttcac gaatgcgcag cgcgaccagc cctgcacctt
    14341 tagcgaccac tttcggtgcg ctcattttgt tttcgtcata ctgcaacgct accgaatagt
    14401 gggtcgggtt attgacaatg acatcagctt tcggcacatc ggccatcatc cgacgccgtg
    14461 cagcagctcg ctgcatctga cggatccgcc ctttaacatg agggtcacct tcgctttgtt
    14521 tgaactcatc acgaatatcc tgccgcgaca tacgcagctt tttcaggtgg ctgaagattt
    14581 ggaaaaagac gtcaaatccc accattggaa tgacaccaag caccaccagc agtgcgcata
    14641 gcccaaccag atccatcgca ttacccatgg cggtagtcgg agactcggcc attaagcgca
    14701 tcatctgcgg ccagtgatgc cagagataaa accccgtcac gctgccaacc aggatggttt
    14761 tcaaaattgc cttaagcaac tccgcgccag tctgtgccga gaacatccgt ttaatgcccg
    14821 gtagcgggtt gagtttggaa aacttcggct gcaaggattt gccgctaaat accagccccc
    14881 ccagcatgac cggagaaatg agcgccacca gcaccacgcc gctaatcagc ggcagcagcg
    14941 ccagcatggc ttctctgatc agcagaataa tctgcccgag gatcagattc gggtca
//
LOCUS       DAESHI010000106        14979 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.107, whole genome shotgun sequence.
ACCESSION   DAESHI010000106 DAESHI010000000
VERSION     DAESHI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14979
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.107"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..266
                     /locus_tag="KW011_003272"
     CDS             <1..266
                     /locus_tag="KW011_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBH7661538.1"
                     /translation="TKAEAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVG
                     LKKAQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
     gene            360..758
                     /gene="fadM"
                     /locus_tag="KW011_003273"
     CDS             360..758
                     /gene="fadM"
                     /locus_tag="KW011_003273"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HBH7661539.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(810..1505)
                     /gene="queC"
                     /locus_tag="KW011_003274"
     CDS             complement(810..1505)
                     /gene="queC"
                     /locus_tag="KW011_003274"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HBH7661540.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1570..3270)
                     /locus_tag="KW011_003275"
     CDS             complement(1570..3270)
                     /locus_tag="KW011_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HBH7661541.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3370..4188
                     /gene="cof"
                     /locus_tag="KW011_003276"
     CDS             3370..4188
                     /gene="cof"
                     /locus_tag="KW011_003276"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HBH7661542.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4340..4798
                     /gene="decR"
                     /locus_tag="KW011_003277"
                     /pseudo
     CDS             4340..4798
                     /gene="decR"
                     /locus_tag="KW011_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
     gene            4828..6600
                     /locus_tag="KW011_003278"
     CDS             4828..6600
                     /locus_tag="KW011_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBH7661543.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6593..8374
                     /locus_tag="KW011_003279"
     CDS             6593..8374
                     /locus_tag="KW011_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBH7661544.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8555..8893
                     /gene="glnK"
                     /locus_tag="KW011_003280"
     CDS             8555..8893
                     /gene="glnK"
                     /locus_tag="KW011_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBH7661545.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8923..10209
                     /gene="amtB"
                     /locus_tag="KW011_003281"
     CDS             8923..10209
                     /gene="amtB"
                     /locus_tag="KW011_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HBH7661546.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="KW011_003282"
     CDS             complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="KW011_003282"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HBH7661547.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11336..11908
                     /locus_tag="KW011_003283"
     CDS             11336..11908
                     /locus_tag="KW011_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HBH7661548.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11939..12250)
                     /locus_tag="KW011_003284"
     CDS             complement(11939..12250)
                     /locus_tag="KW011_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HBH7661549.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12413..12509
                     /gene="ffs"
                     /locus_tag="KW011_003285"
     ncRNA           12413..12509
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KW011_003285"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12629..12982
                     /locus_tag="KW011_003286"
     CDS             12629..12982
                     /locus_tag="KW011_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HBH7661550.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="KW011_003287"
                     /pseudo
     CDS             complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="KW011_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14736..>14979)
                     /locus_tag="KW011_003288"
     CDS             complement(14736..>14979)
                     /locus_tag="KW011_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661551.1"
                     /translation="FFFDVYPRYRYEDIDVLDFRVCYNGEWYNTRFVPAALVEAILNS
                     PRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
BASE COUNT         3470 a         3683 c         4118 g         3708 t
ORIGIN      
        1 aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg
       61 acgaagaagg cacccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga
      121 tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc
      181 cgtttaaaac ggtggaggat ctaaagcagg tgccggggat gggcaattcg ctggtggaac
      241 gtaatctggc ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg
      301 aagaggtcat accagttatg acctctgtac ttataacaac aacgtaaggt tattgcgcta
      361 tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg
      421 cccgctacct tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt
      481 ttcagtggat gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc
      541 gtcgcccagc ggtattaagc gacctgttaa ctattaccag tcagttgcag caattaaacg
      601 gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg
      661 atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag
      721 gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct
      781 caagccgcat ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct
      841 gccatcaccg tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat
      901 gccgcacaat gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta
      961 cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc
     1021 cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt
     1081 agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg
     1141 ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac
     1201 aaaatattac gcccagggac aaacgtattc gggatgccat cggcttcagg ttcataatca
     1261 ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca
     1321 tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt
     1381 tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt
     1441 aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt
     1501 ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg
     1561 accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg
     1621 cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa
     1681 cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa accgctttca gggcattaaa
     1741 tcggttttcc tcatcaggca ttacttgcac cgcgtccagc gtcgattgca gatgtgcata
     1801 tgctggagcg tcgaaaacat gtggccatag tggatcgcaa cgtagccatt gctccagagt
     1861 atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag
     1921 ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc
     1981 tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac
     2041 cagcgtcaag gttttcggta gtttgacttc atccggtact tgccagtgcg gaatagtcca
     2101 gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa
     2161 taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg
     2221 acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac
     2281 ccgttgcagc tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc
     2341 caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg
     2401 acgcaaatgg taataatcat ggctctccag gcgcaccagc tccgctgtga attgtgttaa
     2461 gcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaatagct
     2521 cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg
     2581 atgggtgact tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa
     2641 cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg
     2701 tagggttgaa cgaagataaa agtcccagcg taacccgtca gtagaggttt ccctgtgatg
     2761 cgctaaatcg ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat
     2821 ctgcccggcg agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag
     2881 cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa
     2941 cggctgtaac agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg
     3001 ctgctttccg gtttccagtg cctgttccat catcgcattg cgtagcgatt ccggcgtgac
     3061 cagaaagcgt aattgtccgc gctttccgcg tcctgactgc gcctgccact ccagccatcc
     3121 cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc
     3181 ggccagttcg ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg
     3241 atactggtta agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt
     3301 cactattact tccgtatata tcaggtgata ctcaatctcc attaaccgtg tcacagagtg
     3361 gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac
     3421 catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc
     3481 ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg
     3541 ctggatgcat atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt
     3601 ttacatcgtg atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat
     3661 acccgagcca gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct
     3721 gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa
     3781 atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc
     3841 ttgcagatcc agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccaca
     3901 gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc
     3961 caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc
     4021 gaaatgttag gcagcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc
     4081 gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat
     4141 ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataacg agatcccttc
     4201 cagcaccggg caattgcccg gtttttttgc gttgaatttg tcattttgtg ccgtggtgtt
     4261 taaaccgcac agaataaatt gtcgtgattt cacctttaaa atagaattaa aagagaaaaa
     4321 aattctccgt ggaagggcta tgttagataa aattgaccgt aagctgctgg ccttactgca
     4381 gcaggattgc accctctctt tgtaggcact ggctgaagcc gttaatctga caaccacccc
     4441 ttgctggaag cgcctgaaac ggctggagga cgacggtatc cttatcggca aagtcgccct
     4501 gctggatccg gaaaaaatag gcctcggcct gaccgccttt gtgctgataa aaacgcaaca
     4561 tcacagcagc gaatggtatt gccgctttgt cacggtggtt accgaaatgc cagaagtgct
     4621 ggggttctgg cgcatggctg gtgaatacga ttatctgatg cgcgtccagg ttgccgacat
     4681 gaaacgctac gacgagtttt ataagcgtct ggtaaacagc gtgccggggc tgtcggacgt
     4741 cacttccagc ttcgcgatgg aacagattaa atacaccact tctttaccca tcgaataaat
     4801 atccagaatc aggtcaggac acaacgcgtg cgattatttg ctcaattaag ctggtatttc
     4861 cgtcgggaat ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat cgcgatgctg
     4921 caactggttc cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga acaacacttt
     4981 actaccgggc tgatcctgat gtggatcgcc accatggtgc tgattgccgt tgtggtttat
     5041 ctcctgcgtt atgtctggcg cgtattgctg tttggcgcgt cttaccaact ggctgttgaa
     5101 ctgcgtgaag attattaccg tcagctaagc cggcagcacc cggagtttta cctgcgtcat
     5161 cgcacgggtg atctcatggc tcgtgcgacc aatgacgtcg attgcgtcgt gtttgccgcc
     5221 ggagaagggg tgctgacgct ggtggattca ctggtaatgg gctgcgctgt gttgattatg
     5281 atgtctacgc aaattagctg gcagttgacc ttatttgccc tgttgccgat gccagtgatg
     5341 gcgatcatga ttaagcgcaa cggcgatgct ttgcatgaac gctttaagct ggcacaggcg
     5401 gcgttttcca gtcttaatga ccgcacccag gaaagcctca ccagtatccg catgatcaaa
     5461 gcctttggtc tggaagatcg ccagtcggcg ttatttgccg cggatgccga agataccggc
     5521 aaaaaaaaca tgcgggtggc gcgtattgat gctcgtttcg acccgaccat ctatatcgcg
     5581 attggtatgg cgaacttgct ggcgattggc ggcggtagtt ggatggtggt gcagggcagt
     5641 ttaacgctgg gccagctcac cagttttatg atgtatttag gtctgatgat ttggccaatg
     5701 ctggcgctgg catggatgtt taacattgtg gaacgtggta gtgctgcgta cagccgtatt
     5761 cgtgcgatgc tggcggaagc gccggtggtg aacgatggta gcgaacccgt gccggaaggg
     5821 cgtggcgaac ttgatgtaaa tattcaccag ttcacgtatc cgcagactga ccatcctgcg
     5881 ctggaaaacg tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg cgggccgact
     5941 ggttccggca aaagtaccct gttgtcgctc attcagcgtc atttcgacgt tagcgagggg
     6001 gatattcgct ttcatgatat tcctctgacg aagttacaac tcgatagctg gcgtagccgc
     6061 ctggcggtgg tcagccagac gccattcctt ttttctgaca ccgtggcaaa taacatcgcg
     6121 ctgggttgcc cgaatgccac ccagcaagag attgagcatg tcgcgcggtt agccagcgta
     6181 catgacgata ttttgcgttt accgcaaggt tacgatacag aggtgggcga gcgcggcgtg
     6241 atgctttccg gcgggcaaaa acagcgtatc tctattgctc gtgcgttatt agtcaacgcg
     6301 gaaatcctca tccttgatga tgcgctttcg gcggtggacg gacgcacaga gcaccagatc
     6361 ctgcataacc tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc ccatcgcctt
     6421 tcggcactga cggaagccag tgaaattatt gtgatgcagc acggacatat cgcccagcgt
     6481 ggcaatcatg atgtgctggc gcaacaaagc ggctggtatc gcgatatgta tcgctatcaa
     6541 caactggagg cggcgctcga cgacgctccg gaaaatcacg aggaggccgt cgatgcgtag
     6601 ttttagccaa ctgtggccga ctctcaagcg cctgttagcg tacggttcac cgtggcgtaa
     6661 accgctgggg attgcggtcc tgatgatgtg ggttgcggcg gcggcagaag tcagtgggcc
     6721 gctgcttatc agctatttta tcgacaatat ggtagcgaaa aataacctgc cgttgaaagt
     6781 ggttgcaggg ctggctgcgg cgtatgttgg gctacaactg tttgccgccg ggctacatta
     6841 cgcgcagtcg ctgctgttta atcgggcggc agtaggcgta gtgcaacagt tgcgtaccga
     6901 cgtgatggat gctgcgttac gccagccatt aagcgagttt gatacccaac ccgtcgggca
     6961 ggtgatttcc cgcgtcacta atgacactga agtgatccgc gatctctacg ttaccgtagt
     7021 ggcaactgtc ctgcgcagtg ccgcactggt gggcgcgatg ctggtggcga tgttcagcct
     7081 cgactggcga atggcgctgg tggcgataat gattttcccg gtagtgctgg tggtaatggt
     7141 gatatatcaa cgttacagca cgccgattgt ccgtcgtgtg cgcgcctatc tggcggatat
     7201 caacgacggc tttaacgaaa tcatcaatgg catgagcgtc atccagcagt tccgtcagca
     7261 ggcgcgattt ggcgaacgta tgggtgaggc cagtcgttca cactatatgg cgaggatgca
     7321 aaccctgcgc ctcgacggtt ttctgctgcg tccgctgctg agtctgtttt catcgctcat
     7381 tctttgtgga ttgttgatgc tgtttggctt ctccgccagc ggcaccattg aagtgggcgt
     7441 gctgtatgcc tttatcagct atcttgggcg acttaacgaa cccttaatcg aactgaccac
     7501 gcaacaggcg atgctgcaac aggctgttgt tgctggtgag cgcgtgtttg aactgatgga
     7561 cggaccgcgc cagcaatatg gcaatgatga ttacccgtta cagagtggca ccatcgaagt
     7621 cgataacgtg tcatttgctt atcgcgatga caatctggtg ctaaagaaca ttaatctctc
     7681 tgtgccttcg agtaactttg tggcgctggt cgggcatacc ggcagtggta aaagtaccct
     7741 cgccagttta ttgatgggct attacccgct aacggaaggt gagattcgcc ttgatggtcg
     7801 tccattaagt tcgctaagtc acagcgcgct gcgccagggc gtggcaatgg tgcagcaaga
     7861 tccggtggtg ctggcggata ccttcctcgc caacgtgacg ctggggcggg atatctccga
     7921 agaacgcgtc tggcaggcgc tggaaaccgt gcaactggcg gagctggcgc gtagcatgag
     7981 cgacggtatt tacacgccgc tgggcgagca ggggaataat ctctcagttg ggcaaaagca
     8041 actgctggca ctggcgcgcg tgctggtcga gacgccgcaa atcctgatcc ttgatgaggc
     8101 aaccgccagc attgactccg ggactgaaca ggcgattcaa catgctctgg cggcggtgcg
     8161 tgaacatacc acgctggtgg tgattgctca ccgcttatcg accattgttg atgccgacac
     8221 cattctggtg cttcatcgtg ggcaagccgt ggagcagggc actcaccagc aactgctggc
     8281 tgcccaggga cgctactggc agatgtatca actgcaactt gcgggcgaag agctggcagc
     8341 cagcgtgcgt gaagaggaat cattgagcgc ctgaatagcg caatatttca tcgttggtgc
     8401 aaaaatgtaa cgcactgtgc actgtcatag tgcgttttca ttttcaaact tcttaacttc
     8461 ctgctctctt tctcgttttt catttctggc acaccgcttg caataccttc ttcgtgtagc
     8521 agaaccatta ccgaattctg accggagggg atctatgaag ctggtgaccg tgataatcaa
     8581 accattcaag ctggaagacg ttcgtgaagc gttatcttcc attggtattc agggcctgac
     8641 cgtcaccgaa gtgaaaggtt tcgggcgtca gaaagggcat gccgagctgt accggggggc
     8701 ggaatacagc gtcaacttcc tgccaaaagt aaaaattgat gtggcgattg ctgatgacca
     8761 actcgatgaa gtgatcgata tcgtcagtaa ggcggcttac accggaaaaa ttggcgacgg
     8821 caaaatcttc gtcgctgaat tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga
     8881 agcggcgctg taatctctgg cacacagcaa caggaacgaa aaatgaagat agcgacgata
     8941 aaaactgggc ttgcttcact ggcgatgctg ccgggactgg taatggctgc acctgcggtg
     9001 gccgataaag ccgacaatgc gtttatgatg atttgtactg cgctggtgct gtttatgact
     9061 attccgggga ttgccctgtt ttacggtggg ttgattcgcg gcaaaaacgt gctgtcgatg
     9121 ctgacgcagg tgacggtgac gtttgcactg gtctgtattc tctgggtggt ttacggttac
     9181 tcgctggcgt ttggtgaggg caacaacttc ttcggcaaca ttaactggtt gatgctaaaa
     9241 aacatcgaac tgacggcggt gatgggcagc atttatcagt atatccacgt ggcgtttcag
     9301 ggctcgtttg cctgcattac cgtcggcttg atcgttgggg cgctggcgga acgaatccgc
     9361 ttctcagctg tgttgatttt cgtggtggta tggctgacgc tctcttacat tccgattgcg
     9421 catatggtgt ggggcggtgg tttgctggct tctcacggtg cgctggattt cgcgggtggc
     9481 acggtggtgc atattaacgc cgcaatcgcc ggtctggtgg gcgcgtatct gataggcaaa
     9541 cgcgtgggct tcggcaaaga ggcgtttaaa ccgcacaacc tgccaatggt cttcaccggt
     9601 actgccattc tctatattgg ttggtttggc tttaacgccg ggtcagcggg cacggcgaat
     9661 gaaatcgcgg cactggcatt tgtgaatact gtggtcgcaa cggcggcggc aattcttggc
     9721 tggatcttcg gtgaatgggc gctgcgtggt aagccttctc tgctgggagc gtgttctggc
     9781 gcgattgccg gtctggtcgg cgtgacgcca gcgtgtggct acattggggt tggcggcgcg
     9841 ttgattatcg gcgtggtagc tggtctggcg ggcttgtggg gcgttactat gctcaaacgc
     9901 ttgctgcggg tggatgatcc ctgcgatgtc ttcggtgtgc acggcgtttg tggcattgtc
     9961 ggctgtatca tgactgggat ttttgccgcc agttcgctgg gtggcgtggg cttcgctgaa
    10021 ggtgtgacga tgggccatca gttgctggta cagctggaaa gcatcgccat tacgatcgtc
    10081 tggtccggtg tggtggcatt tattggctac aaactggcgg atctgacggt tggtctgcgt
    10141 gtgccggaag aacaggagcg agaagggctg gacgtcaaca gccacggcga gaatgcctat
    10201 aacgcgtaac aagcactgca aaaaacagcc ggacggtttt cacctccggc tattttttta
    10261 attgtgatta cgcatcaccc cttcctgaac ggtcgaggca accagcacgc cgtcctgagt
    10321 ataaaactca ccgcgcacaa agccgcgtgc gctggacgcc gaggtgctct ccacgctata
    10381 cagcagccat tcattcaaat taaacgggcg atgaaaccac atggaatggt caatggtggc
    10441 aatctgaatc cccggttcga gaaaaccgat gccgtgcggc tgtagagcta ccggcaggaa
    10501 gttaagatca gaagcgtaac cgagcagata ctgatgaacg cgcaggtcat ccggcacgct
    10561 accatttgcg cggatccaca cctgacgatg tggttctgcg acgtgacctt tcagtgggtt
    10621 atgaaactcc accggacgga cttccagcgg acgatcgcag atgaatttat ctttcagcac
    10681 tggcggcagc aggtgcgcca gcgattgggc gatttgtgtt tccgaaggca ggccatcagg
    10741 cgctggcgcg gacggcattg ttttttgatg ttcgaaaccc gcttcaggtg cctggaaaga
    10801 ggccgtcata taaaaaatcg gtttgccgtt ttgaatagca gcaacccggc gggcgctgaa
    10861 gctgttaccg tcacgcagcg tttcgacatc ataaataatc ggcttcttac tatcgccagg
    10921 gcgaagaaag tagctgtgaa acgaatgtac cagccgctct tcagggacgg tctcttttgc
    10981 agcatacaag gcctgaccca cgacctggcc gccaaacacc tggcgtaaac ctaaatcttc
    11041 actctggccg cgaaagagtc cttcctcaat tttttccaga tttaacaatg tcagtaaatt
    11101 ttttagcgcc tgactcatat aactctccag taactaagct gccgcagcaa gccaaagtga
    11161 gttgagtata acgcaaattt gccactggtc cgatgggtgc aatagtctga attacgggct
    11221 aattacaggc agaaatgcgt gatgtgtgcc acacttgttg acgttactat tttgttaacc
    11281 actcttccgg cgaggaaagt tagcccgctg gtgcattgat aataaggaga agtgaatgaa
    11341 actcgtgcac atggccagtt gtttagcggt tgcgattgcg ttggcggctt gcgcagataa
    11401 aagcgcggat attcagacgc cagcaccggc ggcaaatacg tctatttcag caacacaaca
    11461 acctgctatc cagcaaccga atgtctccgg taccgtttgg atccgtcaga aagtcgcact
    11521 gccgcctgat gctgtgctga ccgtgacact ttctgacgcg tcgttagccg atgcgccgtc
    11581 aaaagtgctg gcgcagaaag cggtgcgtac cgaaggtaaa cagtcaccat tcagctttgt
    11641 tctgcctttt aacccggcag atgttcagcc gaacgcgcgt attctgttga gtgcggcgat
    11701 taccgtgaat gacaaactgg tatttattac cgataccgtt cagccggtga tcaacaaggg
    11761 cggaactaaa gccgacctga cattggtgcc gatgcagcaa accgccgtgc cggttcaggc
    11821 cagcggtggt gcaacgacta ccgtaccttc gacttcacca actcaggtga atccgtcttc
    11881 ggcagttcca gctcctacgc aatattaagc gacgttaacc ctctccgccc ggagagggtt
    11941 agtagttcca gcgataacgc tgcaagtcga tttgcccgct tcccgatacc atcacacctt
    12001 ctgccagtaa tgcctgtcgc tgacgctgta aatccggtcc ggttagcgaa attgtgccgt
    12061 ggcgattaac cacccggtgc cagggtaagg tgctgccttc agggagacgc tttaacacac
    12121 cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt cgccacatca ccgtaagtgg
    12181 tgacatagcc ttcgggaata gcggcgacga tttgccagac gcgttgggga aatgaatctt
    12241 ctttttccat cttttcttcc tgaggtaatt tttcagcata atctggaaaa acgcccgagt
    12301 gaagtcgcat tgcgcaagaa accagcatct ggcacgcgat gggttgcaat tagccggggc
    12361 agcagtgata atgcgcctgc gcgttggttc tcaacgctct caatgggggc tctgttggtt
    12421 ctcccgcaac gctactctgt ttaccaggtc aggtccggaa ggaagcagcc aaggcagatg
    12481 acgcgtgtgc cgggatgtag ctggcagggc ccccacccat ttctgcctcc caccgtttcg
    12541 tcaaaaaatc ccaacatagc taaactttaa ccacaactga cgtcgcaaga attgtctggc
    12601 tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt gatggttttg tggttgccgt
    12661 tcctgccgat aaaaaggatg cctatcggga aatggccgct aaggctgcgc cattgtttaa
    12721 agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc gatgtaccgg atggcaaagt
    12781 gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct ttagctggat
    12841 tgaataccct tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt cggacccacg
    12901 gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag cgaatgatct atggcggatt
    12961 cgagtcaatc atcgacgaat aggtagcgtg acgggctgtg cggcgtgccg tcagcccaaa
    13021 aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct ttcggtaatg ccttgctgta
    13081 gagccagccc tgaccgtagt gcacgccatg ctggcgtagc cactcttctt gtttactggt
    13141 ttcgattccc tccgctacca ttttcagttt cagtgttttt gccatttcga tgatatgcgg
    13201 cgtgacattt ttatattcca gcgcatcaac gaaagattta tcgatcttca gaatgtcgac
    13261 atccagatcc tgtaaataac ttaaacttga atatcccgta ccaaaatcat caagataaat
    13321 ttcatggccc gcctcccggt agcgagaaat tatcggggcg ctggttttcg gatcggcaaa
    13381 ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta acctgattga ttgatcattt
    13441 cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa
    13501 tatgctgctg tggatgctga cgcagccagt cgcccatatc ttcaaaggcg cttcttataa
    13561 tcagtagcgt caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg
    13621 gtgacaacca actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt
    13681 tgccattggc taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag
    13741 catcctgcag ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc acaaacatcg
    13801 ctgccagcag gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc
    13861 ctttctgcaa cattttcgtt gaagcccagg tgatgatcga aatattcatc tccgggaagg
    13921 gcaggatatc gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc
    13981 tggtaataat tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc
    14041 caataatggc ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg
    14101 ggtcgatcat gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta
    14161 aatcgttatg cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg
    14221 ggaaggtatc gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga
    14281 tataaaccac ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat
    14341 gggcttcgct acaggcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt
    14401 gtgtcgccac cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa
    14461 taaacgatgt ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata
    14521 ctgaaagaat cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat
    14581 ccttattaac acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata
    14641 aggaggtaga gctggtattt catgaagtga tgggtggaaa cgatcattgt catttgacca
    14701 agcgcagagc tttacgcctg gtatgtaacc ctaacttaag atgttgattc ggcgcgagcg
    14761 agggtaaaaa tatcgtaaaa agacagttca cctttacgga cgatcatttt ttgcagttgt
    14821 tccttatgaa catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt
    14881 acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata
    14941 tcttcatagc ggtagcgtgg gtagacatcg aaaaagaag
//
LOCUS       DAESHI010000107        14941 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.108, whole genome shotgun sequence.
ACCESSION   DAESHI010000107 DAESHI010000000
VERSION     DAESHI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14941
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.108"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            316..1614
                     /gene="kgtP"
                     /locus_tag="KW011_003289"
     CDS             316..1614
                     /gene="kgtP"
                     /locus_tag="KW011_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HBH7661552.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1611..1934)
                     /locus_tag="KW011_003290"
     CDS             complement(1611..1934)
                     /locus_tag="KW011_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HBH7661553.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1980..3335)
                     /gene="pssA"
                     /locus_tag="KW011_003291"
     CDS             complement(1980..3335)
                     /gene="pssA"
                     /locus_tag="KW011_003291"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HBH7661554.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3449..6109)
                     /gene="pat"
                     /locus_tag="KW011_003292"
     CDS             complement(3449..6109)
                     /gene="pat"
                     /locus_tag="KW011_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HBH7661555.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6141..6839)
                     /gene="tapT"
                     /locus_tag="KW011_003293"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6141..6839)
                     /gene="tapT"
                     /locus_tag="KW011_003293"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HBH7661556.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6908..7327)
                     /gene="trxC"
                     /locus_tag="KW011_003294"
     CDS             complement(6908..7327)
                     /gene="trxC"
                     /locus_tag="KW011_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HBH7661557.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7534..8571
                     /locus_tag="KW011_003295"
     CDS             7534..8571
                     /locus_tag="KW011_003295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBH7661558.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8619..9308)
                     /gene="ung"
                     /locus_tag="KW011_003296"
     CDS             complement(8619..9308)
                     /gene="ung"
                     /locus_tag="KW011_003296"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBH7661559.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9613..9996
                     /gene="grcA"
                     /locus_tag="KW011_003297"
     CDS             9613..9996
                     /gene="grcA"
                     /locus_tag="KW011_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HBH7661560.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10052..10639)
                     /gene="eamB"
                     /locus_tag="KW011_003298"
     CDS             complement(10052..10639)
                     /gene="eamB"
                     /locus_tag="KW011_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HBH7661561.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10742..11623
                     /locus_tag="KW011_003299"
     CDS             10742..11623
                     /locus_tag="KW011_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH7661562.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11832..13166)
                     /gene="srmB"
                     /locus_tag="KW011_003300"
     CDS             complement(11832..13166)
                     /gene="srmB"
                     /locus_tag="KW011_003300"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HBH7661563.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13298..14035
                     /gene="trmN"
                     /locus_tag="KW011_003301"
     CDS             13298..14035
                     /gene="trmN"
                     /locus_tag="KW011_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HBH7661564.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14020..>14941)
                     /locus_tag="KW011_003302"
     CDS             complement(14020..>14941)
                     /locus_tag="KW011_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBH7661565.1"
                     /translation="VANLEFNQFPPTALYHPQARNFLLTEALRGEGAYLKRPDGTRFM
                     PDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLGLG
                     IDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSLLE
                     CLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDYVG
                     IVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRKES
                     RGLHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3605 a         3780 c         3913 g         3643 t
ORIGIN      
        1 agcgaaaggc catcctgacg gatggccttt ttgcattggc gcagaaaaaa atgcctgatg
       61 cgacactgcg cgtcttatac tcccacatat gccagattca gcaacggata cggtttcccc
      121 aacttgccca cttccatacg tgtcctcctt accagaaatt tatccttaag ctcctcaata
      181 accattttcc tgctaactaa attcatggtt aaggttgcac aatgatatgc aacaaatgta
      241 taacatttcc tttaccaaaa aaataaacaa aagcgaccga caaaagcatc ggattacggc
      301 aggagacata atggcatggc tgaaagtact gtaacggcag acagcaaact gacaagtagt
      361 gatactcgtc gccgcatttg ggcaattgtg ggggcctctt caggtaatct ggtcgagtgg
      421 ttcgatttct atgtctactc gttctgttca ctctacttcg cccacatctt cttcccttcc
      481 gggaacacga cgactcaact actacaaaca gcaggtgttt ttgctgcggg attcctgatg
      541 cgcccaatag gcggttggct atttggccgc atagccgata aacatggtcg caaaaaatcg
      601 atgctgctat cggtgtgtat gatgtgtttc ggctcactgg taatcgcctg tttgcctggt
      661 tatgaaacaa tcggtacgtg ggctccggca ttattgcttc tcgcgcgttt atttcaggga
      721 ttatccgttg gcggagaata tggcaccagc gccacctata tgagtgaagt tgccgttgaa
      781 gggcgcaaag gtttttacgc atcatttcag tatgtgacgt tgatcggcgg acaactgcta
      841 gccctactgg ttgtcgtggt tttacaacac accatggaag acgctgcact cagagagtgg
      901 ggatggcgta ttcctttcgc gttaggagct gtgttagctg ttgtggcgtt gtggttacgt
      961 cgtcagttag atgaaacttc gcaacaagaa acgcgcgctt taaaagaagc tggatctctg
     1021 aaaggattat ggcgcaatcg ccgtgcattc atcatggttc tcggttttac cgctgcgggc
     1081 tccctttgtt tctatacctt cactacttat atgcagaagt atctggtaaa tactgcggga
     1141 atgcatgtca acgtggcgag tggcattatg actgccgcat tgtttgtatt catgcttatt
     1201 caaccactca ttggcgcgct gtcggataag attggtcgcc gtacctcaat gttatgtttc
     1261 ggttcgctgg cagccatttt taccgttcct attctctcag cattgcaaaa cgtttcctcg
     1321 ccttatgccg cttttggtct ggtgatgtgt gccctgctga tagtgagttt ttatacatca
     1381 atcagtggaa tactgaaggc tgagatgttc ccggcacagg ttcgcgcatt aggcgttggc
     1441 ctgtcatatg cggtcgctaa tgctatattt ggtggttcgg cggagtacgt agcgttgtcg
     1501 ctgaaatcaa taggaatgga aacagccttc ttctggtatg tgaccttgat ggccgtggtg
     1561 gcgtttctgg tttctttgat gctacatcgc aaagggaagg ggatgcgtct ttagtgacgg
     1621 gtcagttgcc agacggtata gccggtgctt gcaccggcga catcccaggc caaatccttc
     1681 cagctccagc cgctcccttc ggggcggcta tcccaaagct cttttgacgc ccccaaactg
     1741 acagagaaca tcaatccaaa catggcactg cgatcccggc tcatcccctg atgctgtgaa
     1801 tattcatttc cggcggcgga aagcatcgcc gaggcgataa agtgttgagc tttatcctgc
     1861 ccgctccagc tatcgttagc catatggctg catccagaaa gtaacaatag cgaacagaca
     1921 aaaagaatac gcaaaacgtg gcctccaaaa aacaaacccc gtacaggacg gggttgtgat
     1981 tacaggatgc ggctaattaa tcggtcgatg cggatacggc gcaaacggcg gatgagttta
     2041 cgaaccttca ccggataatc ggcaatactt tgcagatcgc gatagtgctt aacgatggtg
     2101 gtatgctcgc ggatcagctc cagttctttc tctcgctgcg gcgcaagctt cagttgtgga
     2161 tcgtggatca aaatggcgtt ttccagatcc agacgccagg cgcgcgggtt caggttatta
     2221 ccggtgatca acatccactt atcatcaacc cacatccctt tcaggtgata ggtgttgtcg
     2281 tcatctttcc ataaccgaac cactagctgg tcagtattga cgtaatactg caaacggctc
     2341 aggaaacgac gcagattgat ctcatagaga taaggcaatg cgccaattat cttgaaaggt
     2401 tcatcttccg gaatgtagaa gtcgttcgcg gttttatcac caacaataat ttcgaccttt
     2461 ttcccttcgc gcagcaactg gataatattg cgcacaagga ttgctggcag gttgaagtat
     2521 ggcgtacaga tggttagttt ctgctcggca caaggcataa gatggaaaat ggtcttgttc
     2581 aacagactcg atttccccag ccccactagc ggcgttacgg aaagctgatc gttgtcggca
     2641 tcgccctgga aatgataagc ggcatcacgc agctcctggc ggaacagacg aatatcgttc
     2701 ttgatttccg ggctttttgg ccgattaaca tcatccagac gattaacgcc gcggccattc
     2761 ataatattct gtgtaaccca ttcaaacata atgtctgaca tcttacggtt acggataaga
     2821 tgataacggt cgtagcgata tttatcgtgc tgatgcaggt aaacatcgtt caggctggca
     2881 ccgctataaa gtacgctatc gtcgatgata aagcctttaa agtgcagaac accaagggct
     2941 tcacgggtat tgattggaac gccataaacc ggaacatcta cgcccgaatt ttcctgcgcc
     3001 atgcggcagt accagtcagc gttagtgtta gatgccgcag cgccaatgcg tccacgttgt
     3061 gcacgatgcc agtcgaccag cacccgcaca tccagttccg gacgctgcct tttagcttca
     3121 tacaacgcgt tcagaatgcc tttgccaccg tcatcctgtt cgagatacag ggcgacaatg
     3181 caaatgcgct gcttcgcgct ggctattttt tccagcagcg tctcccggaa gtcggcggga
     3241 gcgtaaaaga aatcgacatc atcaactgat tgagaaatct tgggtagttg ggcaaggtgt
     3301 tgttgatgtt tattacgctt aaattttgac aacatcacag tgcatttctt ctctgttcat
     3361 tgaagggtcc tctgtgcaat gcagacgaca taagcgggca ataataacac cagtcccgat
     3421 taagtggtca acatttccag taccttactc atgattcctc gcgttgggca agatttagcg
     3481 taagcccaac gatcccctct tcgagctgga tatcaacgtt aaacccgagc ttgcgggcta
     3541 gcgccaccat gccacgattg tttggcatcg taataccatt cagacgttgt agtccgtgat
     3601 ctcgcgtata ggtaatcaac ttttccatta agcgtcgacc taagcctaac cctttgagat
     3661 ccgagcgaac cagcacagca aattcggcat cgatgttatc aggatcggaa atcgcacgcg
     3721 tgacgccgag gatctcttcc gtttgatcaa tacgtcgtac cgctacaaac gccatttccc
     3781 gatcgtagtc gatctgtgtc atgttggcta aatcttcatg ggtaaattcg ttgatctcgc
     3841 taaagtagcg gtaataaaga tcttctttgg tgactcgcga aatgaattgc tgaagttgtg
     3901 gctcatcttc tggcaaaatc gggcggaaca agcagcgttc accgtttttc aattctaccc
     3961 attcttccag ctgatgcgga taagggcgca ctgccagccg actctcgtta tcgccttcaa
     4021 acggcgagat atccagcgtg acatccagcg cggtaaattc actgccagaa gccagcaaag
     4081 gatgaatatc cagacgctga atttccgggc aatcgacaat caagttggaa acctgcacca
     4141 gaagctggct caagcctgca acatccaatg ggcgtagcgc actgcgcgca cgaatctttt
     4201 tacttttgat cccctgaata accagatagc gggccaggtt catgttcagc ggcggcagtg
     4261 cgacgacggc ttgatcttca ggacgccact ccacaccgcc ttcacccagc atgatcaacg
     4321 gcccgaaaac cggatcgtgc tcaaccacaa cccgcaactc ctgagcgcca gcacggttag
     4381 ccatactttg caccaacagg ccgtggaccc gcgcctgtgg ccaggccatt tttacgcgat
     4441 cgaaaatagc gttcgccgct tgctggactt cattggctgt acgcaggtaa agcatgacgc
     4501 cctgaacttc cgatttatgt ggaatatccg gcgaacgcaa tttcagcgcc accggataac
     4561 caatctgttc ggcaatatgc accgcttcgg tgctatcgct ggcaatccag gtagggagcg
     4621 tgttcatgcc atacgcttgc aggatgggct gaacttcatg ggtatcgagc gacgtagccc
     4681 cttcggcaat cgcctgttgc aacagaagat gcgcttctgc ggtattggaa gtcagattgc
     4741 tgggcaacgt cggcgtttcg cgtagttgct tctgattacg ccggtactcc accatatgca
     4801 taaaagcagt gatggttcct tccggggtac ggtaggtcgg cagcccggct tcgctgaata
     4861 aacgtcgtgc ctcttgcgag gagtgctcgc cgcaccagtt cgtcagcaaa gagacatatt
     4921 tgctgcgggg atgatgcttt accgcttcaa ttaatacttg cgcgctttct gttgcgggag
     4981 cagcggcgct gggcgaatga ataaccatca gcgcatcaaa atcctggctg tggagcagaa
     5041 tgtccagcgt tttgacataa tgctcgctgc tggcgtcatc gcgcagatcg agagggttag
     5101 agactgccac atgttctggc aatgcatcgc gcaatttctg gcaggtttct tcgcttagcg
     5161 ttgccagctt gccattgcgt gaccataagg catccagcgc cagcgcggca ggcgcagcac
     5221 cgttgctgat aatcatcaac cggtcgccac gcagcgggcg catatggcta agggtttcca
     5281 ccgccgaaaa cagttcgtgg gtatcctgta cccgcaacaa accggcacgc tgaatagccg
     5341 catcccatgc cgggtccatt cctgccgtcg tgttgagcag tcgctgtgcc gccgggctac
     5401 gtccgctttt aatcaccaga atcggtttat tacgcgaggc actacgggct gccgaaacaa
     5461 agcgtcgcgc gtcgcttaac tgttcgagat agagcaggat ggcgctggtt ttactgtcgc
     5521 gcgccagata gtcgagcaat tcatcaacgt cgatatccag gctgtcgccg agcgcaataa
     5581 agtaggaaaa gcccatctca cgctgttgcg cccagtcgag gatggtgttg gagacggctg
     5641 ccgattgtga aataaacgcc agcttgccgc gtttaatcgg cacaggcgaa aagctggcat
     5701 tcagaccttg ccagggagcc agtaaaccca gactgtttgg tccaagcagg cgcatgttat
     5761 ggcgcagggc gcaggcgcgg agatcttcgt gttgcgatgc cggggcggaa agaataatgc
     5821 aggttttaca gcctttctcg ccgagctctt ccagaagagc aagattacgg ctggcattgg
     5881 tacataaaac cgcaaggtcg ggtgtaaagg gcaagctggc aatatccggc caggccaaca
     5941 cacccaacac cgctttccag gctggcgtca ccgggagtac cggtccgtta aagcttcccg
     6001 ccagcaggtt acgcatcatc aggtaacctg cgcgattggg tttcatcgac gcgccaatta
     6061 ccgctatcga ttttggtcgc agtagtgctt ccagtcctcg ctgactcata ccggtctccc
     6121 gcagaagtga ccgaatgatt ttaaacgctt tctaactgtt ctgctgtgat gctacccaga
     6181 tgttgcgttt ttcctgccag atagcgtgtt ttaaagcggg taaaatgctc gcctaaccct
     6241 gctgccgccc cggtatcgcc ggccatatct aacagtgcga tggctacctc ggcagtacaa
     6301 tattggcctg cagcctgggc ttcacgcagg cgataggcag aaagccggga aagatcgacg
     6361 gaaatgacgg gaagattatc cagatacgga cttttacgaa acatcttgcg agcttccggc
     6421 caggtaccat cgagcatgat aaacagcggt ggcttaccgg caggtggtgt gaagatcact
     6481 tcccgttgct catcagcata cgaggcggga aagaccacca ttggctgata atccgggttt
     6541 tgtaccagat ccagcaaatc ctgcgagggt tcggtacgcg accattgaaa cgcaacggta
     6601 tcaggcaaaa tatcagcaat cagacgcccg gtattactgg gcttcattgg ctcggtgtcg
     6661 aacatcagca aacagaagcg actttttgct tgtgctgggg taattgtcga acagagacat
     6721 aatttctctg gcaaaagaca gcgttggcag cgatgaacgc gattaccgcg ggcaagaaaa
     6781 ggacgtgttg cgcgcgcaat acgctcggcg cgtaactgga gaacagcgtt ttcggtcata
     6841 agagagcgtc gaaaaaacgc cattgtcgca gaggagaaaa cggggcacaa gatgcacccc
     6901 ggtaagatta aagagattcg ttcagccagc tatcgaacgg cgctttcggt actgcgccat
     6961 taagcatgtc gacaacctga ccgtttttga aaatcatgat cgtcggtata ctacgaattc
     7021 caaagcgact gctcaattca cgttcagctt cggtattcac tttcacaaag cgcactttac
     7081 cgctacgctc ttgcgcgaca tcttcaaaaa ttggtgcgaa attacggcag gggccgcacc
     7141 acggtgccca gaagtcgatc accacaggta gatcatcctt cagcaatttg tcgagtgttt
     7201 caccggtcgc attaatcacc tctccgtcaa acaagtcgtg accgcagcgt ccgcattttg
     7261 ccgcatcttc gatccgatcg tcgggaatgc gattgatggc ctgacaatgg gtacaaacgg
     7321 tattcataac taacctcggg atgagtaagg agacttatcg gcaacgacgc cggtatgttt
     7381 ctaatatgtt acatattatc ggggacattg ttccagacaa caatctgttt tattcaatag
     7441 gtatgatagt cgcaggcttt ggtacgaaat catggcgaac cgcgcggact tcgggtaatc
     7501 tgcgcgcttc gcgcagcgct ggtggagaaa agcatgaacg atgaaatgaa aggtaaaagc
     7561 ggcaaggtca aagtgatgta tgtccgcagt gatgatgatt ctgataaacg tacccacaac
     7621 ccgcgtaccg ggaaaggggg cgggcgtcca ggaaaatctc gtgctgacgg tggccgtcgc
     7681 cccgcccgcg atgacaaaca gagtcagccc cgtgaccgca agtgggaaga ttcgccgtgg
     7741 cgcacggttt cccgcgcgcc gggtgatgag acgccggaaa aggccgatca cggtggcatc
     7801 agtggtaaaa gttttattga tccggaagtg ttgcgtcgtc agcgtgcgga agaaacccgc
     7861 gtctacggcg aaaatgcctg tcaggcactg ttccagagcc gtccggaagc aattgttcgc
     7921 gcctggttta tccagagcgt aacgccacgt tttaaagaag ccttgcgctg gatggcggca
     7981 aaccgcaaag cgtaccatgt ggtggatgaa gctgaattga caaaagcgtc aggcacggaa
     8041 catcacggcg gcgtttgctt cctgatcaaa aagcgtaacg gtacaaccgt gcagcagtgg
     8101 gtaagccagg caggcgcgca ggattgcgtt ctggcactgg aaaatgaatc taacccgcat
     8161 aacctgggtg gcatgatgcg cagctgcgcg cacttcggcg tgaaaggtgt cgtggtgcag
     8221 gatgcggcac tgctggagtc gggggcggct atccgtaccg cagaaggcgg cgcagagcac
     8281 gttcagccga ttactggcga caacattgtt aacgtgctgg atgatttccg tcaggcaggt
     8341 tacaccgtag tgaccacttc cagcgagcag ggtaaaccgc tgttcaaaac tagtctgcca
     8401 gcgaaaatgg tactggtgct gggtcaggaa tatgaagggt taccggatgc cgcacgcgat
     8461 ccgaacgatc tgcgcgtgaa gattgacggt acgggcaacg ttgccgggct gaatatctct
     8521 gtcgcaaccg gcgttttgct tggtgaatgg tggcgtcaga ataaagcctg ataaatcagc
     8581 cgggtggcaa ctctgccatc cggcatttcc ccgcaaattt actcactctc tgccggtaat
     8641 actggcatcc agtcaatcgg cgtctcgcca cgttgttcca gccactgatt tgccagcaca
     8701 aaatggttgc agccaaagaa tccacgatgc gccgaaagcg gcgacggatg cggtgctttc
     8761 agtacatgat ggcgttgctt atctataatc gcccctttct tttgcgcatg tgatccccac
     8821 aacaaaaaca ccacgccttc gcggtgctgg ttaatcaggc tgatgacttt atcagtaaac
     8881 gtctcccaac cgaggctggc gtgggaatgc gcctgacctg cgcgaaccgt caacacagta
     8941 ttgagcagca gaacgccctg acgcgcccag ctttcgagat agccatgatt agggcgggtg
     9001 aagcccggaa tagtattttc cagctcttta tacatattca ataatgacgg aggaatggca
     9061 atgccgggac gaacggaaaa tgccagacca tgcgcctgtc ccggtccgtg ataaggatcc
     9121 tggccgagaa tcaccacttt aacgtcaccc aactcggtaa agcggaacgc gttaaagaca
     9181 tctttttgtg gtggatagat agtgacgccg gactgccgct cgctggcgac ggtctgaagg
     9241 gtattaagaa aataaggttg ttgcttctct tcagccagca cgtcatgcca ggttaattcg
     9301 ttagccatct cgctctcctg cgaatcttca atccgcctag cttaactgct tctttctaca
     9361 gaacaaaatc atgctcattt tttagactta aacgagtgca aaaaaatgta aattggtaaa
     9421 atttcacacc cgcataagtt gatgtaaaac aataaaatcc atttacgacc acaatcgatg
     9481 tgtggttttt attgatttaa atcaaagaat caagggtggt tgaggagtat atatacactc
     9541 aagcaacaat ggttttacca attggccgcg acaggctgaa caaatcaaat aattttgccg
     9601 gggaggcatc acatgattac aggtatccag attactaaag ccgctaacga cgatctgctg
     9661 aactctttct ggctgctgga cagcgaaaaa ggcgaagcac gttgcatcgt tgcaaaagca
     9721 ggttatgcag aagatgaagt ggttgcagta agcaaactgg gtgacattga ataccgtgaa
     9781 gttccagtag aagtgaaacc agaagttcgc gttgaaggtg gtcaacacct gaacgttaac
     9841 gttctgcgtc gtgaaactct ggaagatgca gttaagcatc cggaaaaata tccgcagctg
     9901 accatccgtg tatccggtta tgcagttcgc tttaactctc tgactccgga acagcagcgc
     9961 gacgttatcg ctcgtacctt tactgaaagc ctgtaaggct taagtagcaa gttactaagc
    10021 ggaagagggc gacctcttcc gctttttttc gttaatagaa aatgcgtacc gcgcaataga
    10081 ccagtaacag ggcaagcacg atatttaact ggcgaccata ctggcgaaac aatcgctgaa
    10141 acagatgccc cgccagcgcc cagcacacat tgccaaacgt cccaatcatc gccagcaaaa
    10201 cgctgacgcc aactacccag cttagcgcct gtgtttgcgg cagaacaaac gtcgacagtg
    10261 ccgtaacacc gtacaaaatg attttgacgt tcacaaactg caaagcaaag ctggcccaaa
    10321 agctgattgg ttttgcctga agtccgtctt cctttgttgg gctggtggcg attttccacg
    10381 ccagccagac aatatatgcc gcccccgccc aactcaaaag gtgtaccgct gccgggtcaa
    10441 tcactgccag tgaaaatgaa atgcccgcac acagtaacat cacaatcaaa aatcccagac
    10501 tcatccctgc cagcacgcgg gtactttgac gaaatccatg cgacgtagca gagctaaggg
    10561 cgagaatatt gttcggtcct ggcgtcatag cggtaatcag ggtgtaagtc caaaaagcac
    10621 ttaaaagggt cggtgtcact tgttatctcc tctgttttgg tagacagtac ccgacacaga
    10681 acatttaaaa aaatgaatgt ttttatattc tagattcgat attttcgata ggtttggggt
    10741 tatggatctg cgccgtttta ttacgcttaa aaccgtggtg gaagagggtt cctttttgcg
    10801 agcttcgcaa aaattgtgct gtacacaatc gacggtgact tttcatattc agcagcttga
    10861 gcaggagttt tcagtccagt tatttgagaa aattggccga cggatgtgcc ttacccgcga
    10921 aggaaaaaag ttgctgccgc acatttatga acttacccga gtgatggata cgcttcgtga
    10981 ggctgccaaa aaggagtcag atccggacgg ggaactacgc gttgtttcgg gcgaaacgtt
    11041 gctctcttac cgaatgccgc aggtgttgca gcgttttcgg caacgcgcac caaaagtgcg
    11101 tttatcgttg cagtcgctga actgttacgt gatccgtgat gcgctactga atgatgaggc
    11161 cgatgtcggc gtcttttatc gcgtagggaa tgatgatgcc ctgaaccgac gagagttggg
    11221 tgaacaatcg ctggtgctgg tggcttcacc acaaattgta gatgtcgatt ttactgagcc
    11281 gggaagacat aacgcctgta gctttattat caatgaacca caatgtgtct tccggcagat
    11341 atttgagagc acgctgcgtc agcggcggat cacggtggaa aacaccattg agcttataag
    11401 tatcgaaagc atcaagcgtt gtgtcgcggc gaatatcgga gtcagctacc tgccgcgttt
    11461 tgcggtggca aaagagttgg agtgcggaga attaattgaa ttgccttttg gcgaacagtc
    11521 gcagaccatt acggccatgt gcgcacacca tgctggaaaa gcggttagcc cggcgatgca
    11581 cacttttatt cagtgtgttg aagagagttt tgtggctgga taaaaataat gtcgggatta
    11641 aacccggcat ttaacttgtc gacaaagtgc gctttattca tgccggatgc ggcgtgaacg
    11701 ccttatccgg cctacaagat cgtgcaaatt caatatattg catgagccac gtaggcctga
    11761 taagcgtagc gcatcaggca gttttgcgtt tgccagcact tttaatacca ggcttaaacc
    11821 cggcattgag attactcttc tgtcgtttgc ggtggcacgc ccgttccgct tggtttacgg
    11881 cgcttaccaa tatttttggt gtcgcgatgg cgttttttca cccgcggttt ctctttctct
    11941 ttagcttttt tcttctcggc gcgttttgcc agtactttct tcgatggctt gccggtctgc
    12001 ttctcgcttg gcgcacgcgt tttcgggcgt aactcatcaa taacgcgagc tttaatcggc
    12061 tcttcaatgt aacggccaac cttgcccagc agcaaatggt catgggcttc caccagcgaa
    12121 attgcggtgc ctttacgacc ggcgcgcgcg gtacgtccga tacggtgcaa ataagtatcg
    12181 ccactgcgcg gcatatcgaa gttaaagacg tggctgacgt caggaatgtc gataccgcgc
    12241 gcggcaacat cggttgcaac cagtacgttt acgcgacctt cggtcaaacg cttgatcgct
    12301 tcgttacgct tgccttgtac catctcacct tcgagatagc agttgttgat gcccgcttcg
    12361 cgcagccagt ttgccagctc atgcacacgt tcacgcttac gcacaaacac aattgagcgg
    12421 gtcgcttccg gctgttttaa cagatgcacc agcaatgcgg ttttatgttc caggtcatcg
    12481 gcgcggtaat accactgatg aatttttttg cgctcacggg tggacggatt ggcagaaacc
    12541 tccaccggat cttccagcag acgctcggca aagtcctgaa tcgcatcgcc ttccagcgtt
    12601 gccgaaaaga gcagggtctg tttacgccag cgcgtttcgc cagcaatatg ttcgatatcc
    12661 tgagcgaagc ccatatccag catacggtct gcttcgtcga ggatcagcgt ttcaaccgcg
    12721 cggcaatcga agttctcttc ttttatgtat tgcagcagac gtccggtcgt ggcgaccacg
    12781 atgtcctgat tttcgctgaa cacttccgcg tggttcatat aggctacgcc gccggtgatg
    12841 gtggcgatat ccagatgcgt atgtttcgcc agttcgcggg catgatcggc cacctgcatc
    12901 gccagctcgc gagttggggt gaggatcaaa atacgcggcg gaccggattt cttacgtggg
    12961 aaatcgagca ggtgctgcaa cgctggcagc agatacgccg ccgttttacc ggtgcctgtc
    13021 ggcgcagaac cgagtacatc acggccatcg agcgcaggcg gaatggcggc agcctgaatg
    13081 gcggtcgggc gagtgaaacc tttatcctgg agggcttcca gcaggctttc gtcgagttca
    13141 agttcggaaa aagtcgttac agtcatgttc tacctctgtg tggggcgctg attatagacg
    13201 ttacggctgc aatcttcatc tgtttgtatg gatatcgctt ctcgggtatt gttctcaccc
    13261 ggtgctatcg ccgtttttcc tgcaaggttt tattttcatg tcacagtcta catccgtgct
    13321 tcgtcgtaat ggatttactt ttaaacagtt ttttgttgct cacgatcgct gtgcgatgaa
    13381 agtgggaacg gatggcattt tgctgggcgc atgggcaccg gtggctgggg tgaaacgttg
    13441 ccttgatatc ggcgcgggta gcgggttgct ggcactgatg ctggcgcagc gaaccagtga
    13501 cagcgtgata attgatgccg ttgagctgga aagtgaagct gccgcgcagg cgcaggaaaa
    13561 tatcaaccag tccccgtggg cagagcggat taacgtccat acggcggata ttcagcagtg
    13621 gctcacacag cagacagtac gcttcgattt aatcatcagt aacccacctt actatcagca
    13681 gggagtagag tgcgcgacac ctcaacggga acaggctcgc tataccacta cgcttgatca
    13741 cccatcgctg ctgacctgtg cggcggagtg cattaccgaa gagggatttt tctgcgtggt
    13801 gctgccagag caggttggta atggttttac tgaactggca ttaagcatgg gctggcattt
    13861 acgtttgcgg acggatgtgg cggaaaacga agcgcgactg ccgcatcggg tgctgctggc
    13921 attctccccg caggcgggag aatgctttag cgatcgctta gtcattcgtg ggccagacca
    13981 gaactattcc gaagcgtata cggcgctgac ccaggctttt tatctgttta tgtaatgatt
    14041 gccgggggga aggatcgatg gaccggaatg cgtgagcagt tccgggtaat ccagcgtaaa
    14101 atgcaggcca cgactctcct tgcgcatcat cgcacagcga acaatcaact cggcaacctg
    14161 taccagatta cgcagctcca gcaaattatt cgacacgcgg aagtgggcgt aatattcgtc
    14221 tatttcctgt tgcaacatat tgatacgccg cagggcgcgc tcaagacgtt ttgttgtgcg
    14281 cacaatgccg acgtaatccc acataaacag ccgtagctcg tgccagttat gctgaattac
    14341 tacccgttcg tcagggttct caacgcggct ttcatcccac ggtggtaacg tactgacgcc
    14401 gtgggcatca ggcatacgtc tggtgatatc ttctgccgcc gaccagccat agaccagaca
    14461 ctccagcaat gaattcgagg ccatgcggtt agcgccgtgt aagccggtat aactcacctc
    14521 gccaatggca tacaagccct cgacgtccgt acgcccatga tcatcaacca ttacaccacc
    14581 gcaggtatag tgtgcagcag gcacaatcgg taccggttct tgtgtgagat caatccccag
    14641 cccgagcaat ttttcataaa tcatcgggaa atgctggcga ataaaatcgg cgggcttatg
    14701 gctgatgtcg aggaacatac aatctgcgcc gaggcgtttc atttcatggt caatggcgcg
    14761 ggcgacaata tcgcgcgggg ccagttcgcc gcgctcatca aaatcgggca taaaacgcgt
    14821 gccatccggg cgcttgagat aagcgccttc gccgcgcagt gcttccgtta acaggaaatt
    14881 gcgtgcctgt gggtgataca gtgcggtagg ggggaactga ttaaattcga gattggcaac
    14941 c
//
LOCUS       DAESHI010000108        14297 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.109, whole genome shotgun sequence.
ACCESSION   DAESHI010000108 DAESHI010000000
VERSION     DAESHI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14297
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.109"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            19..1236
                     /gene="rtcB"
                     /locus_tag="KW011_003303"
     CDS             19..1236
                     /gene="rtcB"
                     /locus_tag="KW011_003303"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HBH7661566.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRDLAY
                     FMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEEINC
                     HHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCSCSH
                     GAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSDLVE
                     VIYTLRQVVCVKG"
     gene            1240..2256
                     /gene="rtcA"
                     /locus_tag="KW011_003304"
     CDS             1240..2256
                     /gene="rtcA"
                     /locus_tag="KW011_003304"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HBH7661567.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(2299..5004)
                     /gene="malT"
                     /locus_tag="KW011_003305"
     CDS             complement(2299..5004)
                     /gene="malT"
                     /locus_tag="KW011_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HBH7661568.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            5615..8008
                     /gene="malP"
                     /locus_tag="KW011_003306"
     CDS             5615..8008
                     /gene="malP"
                     /locus_tag="KW011_003306"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HBH7661569.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLVGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            8018..10102
                     /gene="malQ"
                     /locus_tag="KW011_003307"
     CDS             8018..10102
                     /gene="malQ"
                     /locus_tag="KW011_003307"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HBH7661570.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10147..11463)
                     /gene="gntT"
                     /locus_tag="KW011_003308"
     CDS             complement(10147..11463)
                     /gene="gntT"
                     /locus_tag="KW011_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBH7661571.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(11824..12399)
                     /gene="nfuA"
                     /locus_tag="KW011_003309"
     CDS             complement(11824..12399)
                     /gene="nfuA"
                     /locus_tag="KW011_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HBH7661572.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(12458..13141)
                     /gene="gntX"
                     /locus_tag="KW011_003310"
     CDS             complement(12458..13141)
                     /gene="gntX"
                     /locus_tag="KW011_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HBH7661573.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            13179..13949
                     /gene="bioH"
                     /locus_tag="KW011_003311"
     CDS             13179..13949
                     /gene="bioH"
                     /locus_tag="KW011_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HBH7661574.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(13978..>14297)
                     /locus_tag="KW011_003312"
     CDS             complement(13978..>14297)
                     /locus_tag="KW011_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HBH7661575.1"
                     /translation="LMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDG
                     VAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAA
                     ASQ"
BASE COUNT         3440 a         3565 c         3992 g         3300 t
ORIGIN      
        1 cgaaaagaga aaaacaaaat gaattacgaa ttactgacca ctgaaaatgc cccggtaaaa
       61 atgtggacca aaggcgtgcc ggtagaagcc gatgcgcgtc agcaacttat taatacggcg
      121 aagatgccgt ttattttcaa acatattgcg gtaatgcctg atgtacacct gggtaaaggt
      181 tccaccattg gtagcgtgat cccgaccaaa ggggcgatta ttccggcggc ggtgggcgtg
      241 gatattggct gtggaatgaa cgcgctgcgt accgcgttaa cggcggcaga cctgcctgaa
      301 aacctggcgg agctgcgtca agcgattgaa acggccgtac cacacgggcg tactaccggg
      361 cgttgtaaac gtgataaagg cgcatgggaa aacccgcctt ttaacgtcga tgcgaaatgg
      421 gctgagctgg aggccggtta tcagtggtta acgcaaaaat atccgcgttt ccttaacact
      481 aacaactata aacacctggg aacgggtaac cactttattg aaatctgcct tgatgagtcg
      541 gagcaggtgt ggattatgct gcactccggt tcacgcggaa ttggtaacgc catcgggact
      601 tactttatcg atctggcgca aaaagagatg caggaccaac ttgagacgtt gccgtcgcgt
      661 gatctggcgt actttatgga aggtacggaa tactttgatg attacctaag agccgtggcc
      721 tgggcgcagc ttttcgccag ccttaaccgc gatgcgatga tggaaaacgt ggtaacggca
      781 ttgcagagca ttacgcagaa aacggtcaga cagccacaaa cgctggcgat ggaagagatc
      841 aactgtcacc acaactatgt gcaaaaagaa cagcactttg gtgaagagat ctacgtgacg
      901 cgtaaaggcg cggtgtttgc gcgtgctggt caatatggaa ttattccagg gtcgatggga
      961 gcaaaaagct ttatcgtccg tgggctggga aatgaagaat ctttctgttc gtgcagccac
     1021 ggtgccgggc gggtaatgag ccgtactaaa gcgaaaaaac tgttcagcgt ggaagatcaa
     1081 attcgtgcca ccgcgcatgt ggaatgccgt aaagatgccg aagtgatcga cgaaatcccg
     1141 atggcgtata aagatattga tgcggtgatg gcggcacaaa gcgatctggt ggaagttatc
     1201 tataccctgc gtcaggtggt gtgcgtaaaa ggataaatga tgaaaaggat gattgcgctg
     1261 gatggcgcac agggcgaagg tggcgggcag atcctgcgct cggcgctgag cctgtcgatg
     1321 ataaccggcc agccatttac catcaccagc attcgtgccg ggcgggcgaa accggggctg
     1381 ttgcgccagc atctgaccgc ggtaaaagcg gctgcggaaa tttgtagggc aacggtggaa
     1441 ggcgcggagc tggggtcgca gcgtctggtc ttccggcttg gcaccgtgcg cggcggcgag
     1501 taccgctttg ctatcggtag tgccggaagc tgtacgctgg tgctgcaaac ggtgctgccc
     1561 gcgctgtggt ttgccgatgg accttcgcgt gttgaagtga gcggaggcac cgataacccg
     1621 tcggccccgc ctgcggattt tatccgccgg gtgctggagc cgctgctggc gaaaatagga
     1681 attcatcagc aaaccacgct gttacgtcac ggtttttatc ctgccggagg cggcgtggtg
     1741 gcaacggaag tctcgcccgt ggcatcgttt aacaccttgc aacttggcga gcgcgggaac
     1801 attgtgcaga tgcgtggaga agttctatta gctggtgtgc cgcgccatgt tgctgagcgt
     1861 gaaatcgcta cactggcggg gagtttttcc ctgcatgaac agaatattca taacctgccg
     1921 cgcgaccagg ggccgggtaa taccgtctcg ctggaagtcg aaagtgaaaa tatcactgaa
     1981 cgctttttcg tcgtcggtga aaagcgcgtc agtgccgagg tggtagccgc gcagttagtg
     2041 aaagaggtga aacgctacct ggcaagcccg gcggtagtgg gggaatatct cgccgaccag
     2101 ctggtgctac cgatggcgct ggcgggcgcg ggagaattta cggtcgccca tccttcatgc
     2161 catctgctga ccaatatcgc cgtggtggag cgtttcttgc cggtgcggtt tggtttgata
     2221 gaaacagatg gcgtaacgcg ggtgagtatt gaatgatgta aagatgtaaa ccggatccaa
     2281 tgcattatcc ggctaaactc acaccccata tcccatcatc ttcagcagtt tctgcgcgtg
     2341 ttgtaccgcg gcctgacggt gggcgacgcc gagtttttgg tacagattgc ggatatgcgt
     2401 cttgatggtg gttgccgcca cttccagctc gcccgcaatt tgctcattgc tgtaaccgga
     2461 gtagatcagc cccaggacct gccattcacg ttgggtcaac gggctggtgc ggatcagttc
     2521 tggcacttcc ggatgattca gcagacgttc aacgaaattc tcatcgaaat gggcgaattt
     2581 atgccgatga tgttgattga tttctcgcag aatacgctgc gcgcgatgct gttccagttc
     2641 cggcagcgta ttgagttgaa tcagctggcg cagttgttgc gccatcgctt cgccttcgat
     2701 gacaaaatga ctgataaatc cggtgcgatt cgccagtttt aatgcgtcca gcaacacgcg
     2761 ctgggcgtca cttttacgtc cggcctgcca gtacagttga ttaagcagca acaggttacg
     2821 gttgagatcg ctcatcaacc gcagactgcg ggcattttca ttgagttctt cgagaacaat
     2881 ttccgcaggt tcaaactcgc ccagcaagat ttgtgcgcgg gcaatgttgc gccattgacc
     2941 ttgcaggaag tggttgttcg caaactccgg tttagccgta tgacgcaacc agttggcagc
     3001 ggcggcttta tcgccggtca tttgccagta aatcacccgg actttgttgg cgttagagat
     3061 ccagtcgctg tgatatttgc cattccccag caggttttcc agacggttca gctggctacg
     3121 ggcgttatct aaatcaccac gggccagcga gcattgaatc aacattgcca ggcactgaag
     3181 ctgttgctgt ggctgataag acgacaagac ttcaatcccg ctacgcgccg acgcttcggc
     3241 ttcatccagc cgtgcccagg cccataacag ttgtgctcgg atgcgcacca gaaactcatg
     3301 catcggcagc tgttccagat gctgctcgtt gatcagctgg aacgtttttt cctgtgtttc
     3361 ccacgcggtt tgcaggaacc cttgggcaaa cagaatttca ctttgctgga ttaaactcca
     3421 taaggcgtag tgccagacgt cgtgctggcg tgccatctgt tcggtttgct gcattagcgc
     3481 cagtgagcgg gtcaattcgc ctttgcagtg cagcacttca cccagcaccg aggttgctac
     3541 aatgcggcta tagaaccagc ccggtggcag ctcttccagt gccagttttg ccagccgttc
     3601 cgcttcatcc ggattaccat cgttaatcgc cacctgggcg cgcagagcgt taaattctgc
     3661 gtgcatggtg ccttctctga tgtccttgat ttcatgttca gcacgggcta gcagggtgtt
     3721 aacttcgcca tagcgatgtt ggctttgcat tagccacgcc tgcaataaca ctaactgcgg
     3781 attttccagc aaactgtccc acggcagggc cttaagcgac tcttccagca gcgacagttc
     3841 gctatggttg aacaaactcc aggcgtgatt aagcagaata tcgcgcagca tcagcgcatc
     3901 gcctgccgcc agcgcatgat gaatcgcttc gctgggaaat ccctgggcca tccagctttc
     3961 tgcggcggca cggtggattt ccggcagctc cgccgccagt tcccactggc agcgctggcg
     4021 caggaagttg ccaaacagcg ggtgatagca gaaccactcg ccggtatcat ccatccgctg
     4081 taaaaacagc ccctgacgct caatctcttc gagacgcatt tgcccgtttt cttcgccagt
     4141 cacacgggtg atgagggcat cgttcattga gcgcaaaatg gcgcttttca acagaaaatg
     4201 gcgcgttgcg agatcgacgt tatccaaaac ctcatcgacc agataatccg aaagatggct
     4261 ggcattgatt cccgccaggc ggcgtgccga cttatgggct gagtgggtat tctgccgggc
     4321 ggagagggcg attagctgta gtgccgtcgc ccaaccggaa acgtcatcgc aaatccaact
     4381 gctttctgcg gcttcaatcg gcgatgacag acggcaatca aaaaactgct tcgcttcctg
     4441 atgggtaaat gccagttgct gactgccaat ttccagcagt tggtcacgaa cacgcagatt
     4501 ggcaatgccc agttgcggaa ggttgcgtga caacaccacc agggtgagat tttctggttg
     4561 atggcgaata aagaagcgca ttgactcgtg gatcactgga ttagtgatca gatgatagtc
     4621 atcgatgacc agataaagtg ggctatgcca ttccgccagc tcaatgaaaa gctgggcgaa
     4681 gagtgacgtc aggctggcat attgccgttt ttgcgccatc gtctcacata tcgcacagtg
     4741 accgttggtt gcctgctgca cggcggcaat gagatagctg gcgaaacgct cttgctggtt
     4801 atcaccttca tccagcgagt accagccgat atcgtttttg cctgccgccc actgggaaat
     4861 gagggtggtc tttccgtagc ccgcaggact cgtgatcagc gccagccgga agttgttcgc
     4921 gccggaaagt ttagccagca ggcgctcacg aaccacggta tggtcgagtc gaaccggacg
     4981 acttagtttt gacggaatca gcatagttaa tcacttcact gtggaaaatg aggaaatatt
     5041 atttttttgc gcttcgtaat taatggttat aaggtcggcc agaaaccttt ctaatgcaag
     5101 cgatgacgtt tttttatgtg tctgaatttg cactgtgtca caattccaaa tctttattaa
     5161 caactcacct aaaacgacgc tgatccagcg tgaatactgg tttcccttat gttcatcaga
     5221 ttcatttaag caagggtttc ttcttcattc ctgatgaaag tgccatctaa aaagatgatc
     5281 ttaataaatc tattaagaat gagatggagc acattggata ttttacttgt gaaactgttt
     5341 cactccttta cttaatttat agagttacct tccgcttttt gaaaatacgc aacggccatt
     5401 ttttgcactt agatacagat tttctgcgct gtattgcatt gatttgctgc taatcctgtg
     5461 gtttgcacta gctttaagtg gttgagatca catttccttg ctcatccccg caactcctcc
     5521 ctgcctaatc ccccgcagga tgaggaaggt caacatcgag cctggcaaac tagcgataac
     5581 gttgtgttga aaatctaaga aaagtggaac tcctatgtca caacctattt ttaacgataa
     5641 gcaatttcag gaagcgcttt cacgtcagtg gcagcgttat ggcttaaatt ctgcggctga
     5701 aatgactcct cgccagtggt ggctagcagt gagtgaagca ctggccgaaa tgctgcgtgc
     5761 tcagccattc gccaagccgg tggcgaatca gcgacatgtt aactacatct caatggagtt
     5821 tttgattggt cgcctgacgg gcaacaacct gttgaatctc ggctggtatc aggatgtaca
     5881 ggattcgttg aaggcttatg acatcaatct gacggacctg ctggaagaag agatcgaccc
     5941 ggcgctgggt aacggtggtc tgggacgtct ggcggcgtgc ttcctcgact caatggcaac
     6001 tgtcggtcag tctgcgacgg gttacggtct gaactatcaa tatggtttgt tccgccagtc
     6061 ttttgtcgat ggcaaacagg ttgaagcgcc ggatgactgg catcgcagta actacccgtg
     6121 gttccgccac aacgaagcac tggatgtgca ggtagggatt ggcggtaaag tgacgaaaga
     6181 cggacgctgg gagccggagt ttaccattac cggtcaagcg tgggatctcc ccgttgtcgg
     6241 ctatcgtaat ggcgtggcgc agccgctgcg tctgtggcag gcgacgcacg cgcatccgtt
     6301 tgatctgact aaatttaacg acggtgattt cctgcgtgcc gaacagcagg gcatcaatgc
     6361 ggaaaaactg accaaagttc tctatccaaa cgacaaccat actgccggta aaaagctgcg
     6421 cttgatgcag cagtacttcc agtgtgcctg ttcggtagcg gatattttgc gtcgccatca
     6481 tctggtgggg cgtaaactgc acgaactggc ggattacgaa gttattcagc tgaacgatac
     6541 ccacccaact atcgcgattc cagaactgct gcgcgtgctg atcgatgagc accagatgag
     6601 ctgggatgac gcttgggcca ttaccagcaa aactttcgct tacaccaacc ataccctgat
     6661 gccagaagcg ctggaacgct gggatgtgaa actggtgaaa ggcttactgc cgcgccatat
     6721 gcagattatt aacgaaatta atactcgctt taaaacgctg gtggagaaaa cctggccggg
     6781 cgatgaaaaa gtgtgggcca aactggcggt ggtgcacgat aaacaagtgc atatggcgaa
     6841 cctgtgtgtg gttggcggtt tcgcggtgaa cggtgttgcg gcgctgcact cggatctggt
     6901 ggtgaaagat ctgttcccgg aatatcacca gctatggccg aacaaattcc ataacgtcac
     6961 caacggtatt accccacgtc gctggatcaa acagtgcaac cctgcactgg cggctctgtt
     7021 ggataaatca ctgcaaaaag agtgggctaa cgatctcgat cagctgatca atctggaaaa
     7081 attcgctgat gatgcgaaat tccgtcagca atatcgcgag atcaagcagg cgaataaagt
     7141 ccgtctggcg gagtttgtga aagttcgtac cggtattgag atcaatccac aggcgatttt
     7201 cgatattcag atcaaacgtc tgcatgagta caaacgccag cacctgaatc tgctgcatat
     7261 tctggcgttg tacaaagaaa ttcgtgaaaa cccgcaggct gatcgcgtac cgcgcgtctt
     7321 cctcttcggc gcgaaagcgg caccgggcta ctacctggcg aagaatatta tctttgcgat
     7381 caacaaagtg gctgacgtga tcaacaacga tccactggtt ggcgataagt tgaaggtggt
     7441 gttcctgccg gattattgcg tttcggcggc ggaaaaactg atcccggcgg cggatatctc
     7501 cgaacaaatt tcgactgcag gtaaagaagc ttccggtacc ggcaatatga aactggcgct
     7561 caatggtgcg cttactgtcg gtacgctgga tggtgcgaac gttgaaatcg ccgagaaagt
     7621 cggtgaagaa aatatcttta tttttggtca taccgtggaa caagtgaagg caattctggc
     7681 caaaggctac gacccggtga aatggcggaa gaaagataag gtgctggacg cagtattgaa
     7741 agagctggaa agcggtaaat acagcgacgg cgataagcat gccttcgacc agatgctgca
     7801 cagtatcggc aaacaaggcg gcgatccgta tctggtgatg gcggatttcg cagcctatgt
     7861 tgaggcacaa aagcaggtgg atgtgctgta ccgcgaccag gaggcctgga ctcgcgcggc
     7921 gatcctcaat accgcccgct gcggtatgtt tagctcggat cgctctattc gcgattatca
     7981 ggctcgtatc tggcaggcaa aacgctaagg aagctcgatg gaaagcaaac gtctggataa
     8041 tgccgcgctg gcggcgggga ttagccccaa ttacatcaat gcccacggta aaccgcagtc
     8101 gattagcgcc gaaaccaaac ggcgtttgct tgacgcgatg catcaacgta ccgccacgaa
     8161 agtggcggta acgccagtcc cgaatgtcat ggtttatacc agcggcaaaa aaatgccgat
     8221 ggtggtggag ggcagcggcg aatatagctg gctgctgacc accgaagaag gaacacagta
     8281 caaaggccat gtaacggggg gcaaagcgtt caatctaccg acgaagctgc cggaaggtta
     8341 tcacacgctg acactcaccc aggacgacca gcgcgcgcat tgccgggtga ttgtcgcccc
     8401 gaaacgctgt tacgaaccgc aggcgttgct gaataaacaa aagctgtggg gtgcctgcgt
     8461 tcagctttat acgctgcgat cggaaaaaaa ctggggtatt ggggattttg gcgatctcaa
     8521 agcgatgctg gtggatgtgg caaaacgtgg cgggtcgttc atcggcctga acccgattca
     8581 tgcgctctat ccggcaaatc cggagagtgc cagcccatac agcccgtctt ctcgccgttg
     8641 gctgaatgtg atttatatcg acgttaaagc cgtcgaagat ttccatctta gcgaagaggc
     8701 tcaggcctgg tggcagttgc cgaccacgca acagacgctg caacaagcgc gggatgccga
     8761 ctgggtcgat tactccacgg ttaccgccct aaaaatgaca gcattacgaa tggcgtggaa
     8821 aggtttcgcg caacgtgatg atgagcagat ggccgcgttt cgccagtttg ttgcagatca
     8881 aggcgacagc ctgttctggc aggcagcctt tgatgcgcta catgcccagc aagtgaaaga
     8941 ggacgaaatg cgctggggct ggcctgcatg gccagagatg tatcagaacg tggattcacc
     9001 agaagtgcgt cagttctgcg aagaacatcg taatgacgtc gatttttatc tctggttgca
     9061 gtggctggct tacagccagt ttgccgcctg ctgggagata agccagggct atgaaatgcc
     9121 gattggcttg tatcgtgatc tggcggttgg cgtagcggaa ggtggggcgg aaacctggtg
     9181 tgaccgtgaa ctatattgcc tgaaagcgtc ggttggcgcg ccgccggata tcctcggccc
     9241 gttggggcag aactggggat taccgccaat ggacccgcat atcatcaccg cgcgtgccta
     9301 tgaaccgttt atcgagctgt tgcgtgccaa tatgcaaaac tgcggcgcat tacgaattga
     9361 ccatgtgatg tcgatgctgc gtttgtggtg gataccgtat ggcgagacgg cagatcaggg
     9421 cgcgtatgtt cactatccgg tggatgatct gctctcgatt ctggcactcg aaagtaaacg
     9481 tcatcgctgt atggtgattg gtgaagatct cggtaccgta ccggtagaga ttgtcggtaa
     9541 gctgcgcagc agcggtgtgt actcctacaa agtgctctat ttcgaaaacg accacgagaa
     9601 gacgttccgt gcaccgaaag cgtatccgga gcagtcgatg gcggttgcgg cgacacatga
     9661 cctgcctacg ctacgcggtt actgggagag cggggatcta acgctgggca aaaccctggg
     9721 gctgtatccg gatgaagtgg tactgcgcgg tctgtatcag gatcgcgaac tggcgaagca
     9781 agggctgctg gatgcactgc ataaatatgg ttgtctgcca aaacgtgccg ggcataaggc
     9841 atcgttgatg tcgatgacgc cgacgctgaa ccgtggtttg cagcgctaca ttgccgacag
     9901 taacagtgct ctgttaggac tacagccgga agactggctg gatatggccg aaccggtgaa
     9961 tattcctggc accagttacc agtataaaaa ctggcgacgc aagctttccg caacgcttga
    10021 gtcgatgttt gccgatgatg gcgtgaacaa gttgctgaag gatttggaca gacggcgcag
    10081 agctgcagcg aagaagaagt agagtgcggt tgatgccgga tgcgatgcat ccggcagtca
    10141 gtgttatcaa atcaccatat tcagcagcag acagcctacc agaccgcaca ccgagataat
    10201 ggtttccagc atcgaccagg atttgatggt ctcgccgata gtcaggttaa agtactcttt
    10261 gaacagccag aagcccggat cgttcacatg agagaaaatc acactaccgg aacccaccgc
    10321 aataaccatc agctctgggc taacacccgt cgttgcaatc agcggtgccg cgataccacc
    10381 cgcagtgatt gccgcaacgg ttgcggaacc cagcgcgata cgcagtacgg cagcaatcga
    10441 ccaggccatc agcagcggag aaatgttggt ttcgtgcatc atggaagcaa tgtatttgtc
    10501 cacgccgctg tctaccagca cctgcttgaa cgcaccgcca ccaccgatga tcaacagcat
    10561 catcgcaatg attttgatgg aagaaaccag cgtgtcgtta atctgatcca ttgaacgacc
    10621 acggttcaga ccaaaggtga acatcgcaat cagcacggca atcagcgttg ccattaccgg
    10681 gtcaccgagg aactccgcta ccggcaggaa agcgtgacct ttcggcagga tcatttcggc
    10741 aatcgcacgc atcgccatca gcacaaccgg caccagagaa gtccagacgc tgacgccaaa
    10801 gctcggcatc tcttcttcgc tgaaggtttt cgcgctgtag agaccttccg ggattggctt
    10861 atcgatacct ttcaacacgc gagcgtaaac cggaccggcg agaatcacgg tcgggattgc
    10921 cagaatagta ccgtacagca gggttttacc catatcggca ttgaaaatgg tggcaatcgc
    10981 agtcgggccc ggatgcggtg gcaggaagcc gtgggtcaca gacagtgcag ccgccattgg
    11041 tacaccaaca tacagcagtg gaatattcgc ggaagccgcg atggtaaaca ccagcggcag
    11101 catcagcacg aagcccactt catagaacag ggcaaaacca acggtaaaac cggttaacac
    11161 caccgcccac tggatgtgct ttttaccaaa tttggcaatc agcgtggtgg cgatacgttg
    11221 tgcgccaccg cagtctgcca gcattttgcc cagcattgcg ccaaaaccca tgatcagggc
    11281 aaggctaccg agcgtcccgc cgacaccgac tttgatggag tcaataactt tatccagcgg
    11341 cattccttgc attaatccaa cagcaagcgc cacgaggacg agagcgatga agccgttcat
    11401 tttgaagcgg atcatcagga gcaacaacaa gattacaccg atagcaacaa tgactaatgg
    11461 catgatttac ctggcctttc atttgttatg ggtaacgtca attttctgac gacaaactct
    11521 aattatccca atcgggaaca gagatattgc ggcaccacga ctgataccca ctaaaactaa
    11581 ttattgtagt cagatgtcag gagtatgttt ggtacccatg tgaatgatac gggtaacatt
    11641 tggcgtttga gaatcaccag agcggggtaa atttaaatta tgagaggttg gtcatattat
    11701 cgcggggaaa cgaagggagg gatttgccaa agcaatgctg tgccaacgtc tggcacatgt
    11761 tcaacgtagg cccgaaatga cgctttagcg tcgcatcggg caatctacaa aagaggggat
    11821 aacttagtag taggagtgtt cgccgcgctg gtgttcggtg agatcgcgca cacctttcag
    11881 ctccgggaat tcgttcagca gctgcttctc gatcccttct ttcagcgtca catcgaccat
    11941 ggaacaaccg ttacagccgc cgccaaattg cagaatggcg taaccgtctt cggtgatttc
    12001 catcagcgaa acgcgaccac cgtgaccagc aagctgtggg ttgatctgcg actgcagcat
    12061 atactccacg cgctccatca gcggtgcatc gtctgccact ttacgcattt tggcgttcgg
    12121 ggctttcagc gttaactgag aacccaactg gtcggtaaca aagtcgatct ctgcatcttc
    12181 caggtatggt gcgcttaact catcaacata cgcggtcagc aggtcaaatt tcagggcagt
    12241 gtcggtggct tccacggcat ccggcggaca ataagaaaca ccacattcag cgttaggcgt
    12301 gccagggtta atcacaaata cgcggatttg tgtcccttct tcctgatttg ccagcagttt
    12361 ggcaaagtgc gcttgtgcag catcggaaat acggatcata gtaatggcct aatagttgac
    12421 tattttagtt ggttataata cgcccatcat cgaggctcta caaggttcga caaaggcacc
    12481 agacctggac agtcgccgca ccattgcgta aaagcaactg cgcaatctct gcgacggtac
    12541 ttccggtggt aacgacatca tccacaatca ccatatggcg accttgcacg ggcaattcaa
    12601 gacgaaaggc atttttcagg ttgcgcttgc gcagccgggc actgagaaaa tgctgggtcg
    12661 cagtggcccg tgtacgtgtg acggcttcgc tatcccattg gcagtgcaac cagcgtgata
    12721 acggctgaca cagcaaatcg ctctgattaa atccccgacg ccagtgacgc cgctgccata
    12781 acggaacgct gatgatgcga tccggcaatt gcaacccggt ggtgcgacga gcgtgtaaga
    12841 cttccaatag taacagacgt gacagggcgc tggcgatttc actgcgccgg gaaaatttaa
    12901 gctggtggat gagcagactt aacggcggcg catagtcggc aaccgtgacc agtctttgcc
    12961 agggcggcgg tttttgcagg cagcgaccgc agggaagatg ggagtgtgtg gcgggtaatc
    13021 cacattgtgg gcataacgtt ttatctgtgc gggtggcgcg tgaacagacc gaacaaatcc
    13081 cccaatgacc taacgccagt ggcattcggc atagccagca taatcccggt actgttagca
    13141 tatgttcatc cttgtaagtc aaaagagaac aatagcggat gaataacatt tggtggcaga
    13201 ccaaaggtca ggggaatgtt catcttgtgc tgctgcacgg atggggactg aatgccgaag
    13261 tgtggcgttg cattgacgag gaacttagct cgcattttac gctgcacctt gttgatctgc
    13321 ccggcttcgg gcgcagccag ggatttggtg cgctgtcact tgctgatatg gccgaagccg
    13381 tgctgcaaca ggcacctgat aaagccattt ggttaggctg gagtctgggc gggctggtgg
    13441 caagccagat tgcgttaacc catcccgagc gtgttcaggc gctggtcacc gtggcgtcgt
    13501 caccttgttt tagtgctcgt gacgattggc cggggataaa accggacgtg ctggcgggat
    13561 ttcagcagca actcagtgat gattttcagc gtacagtgga gcggttcctg gcgttacaaa
    13621 ccatggggac tgaaacggcg cgccaggatg cgcgggcgtt gaagaaaacc gttctggcgt
    13681 taccgatgcc ggaggttgac gtgcttaatg gcgggctgga aatcctgaaa acggttgatc
    13741 tccgtcagcc gctgcaaaac gtgcccatgc cgtttttgcg attgtatggc tatctcgatg
    13801 gtctggtgcc gcgcaaagtg gtgccgatgc tggataaact ctggcctcgc agcaaatcat
    13861 atatcttcgc caaagcggcc catgcgccgt ttatttcgca tccggtcgag ttttgtcacc
    13921 tgctggtggc gttgaagcag agggtgtagg caacttttga aatggcgaga cagaaattta
    13981 ctggctcgcc gcagccaact cttcttctga caccccggta aagcgcatga tgtccgcaag
    14041 agggactccg gattcaagca ttattttggc tatatgcagg gctttggatt gttccccctc
    14101 ctgccgcaat ctttccgcaa ttgtcattaa actctccttg tgtttcggtg aacgttcggc
    14161 aacgccgtcg ataaaatcgt taaaacgtac cgcgtcgccg gtttgcagta tgtaattaaa
    14221 cagccctttg atttgtctgt cattagcgta tccactactt aataagcagg ccatttgctc
    14281 taccagcccc atcaggt
//
LOCUS       DAESHI010000109        14124 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.110, whole genome shotgun sequence.
ACCESSION   DAESHI010000109 DAESHI010000000
VERSION     DAESHI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14124
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.110"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(270..821)
                     /locus_tag="KW011_003313"
     CDS             complement(270..821)
                     /locus_tag="KW011_003313"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661576.1"
                     /translation="MNIDNWAIPITSLGGQLLGVELVSHVEREGIRQIGLWEEEVLHG
                     QLMLIESKAAWFRDNGLYCVITTGRGEPYEYLPFLRQMTRDMKVHNQQWLDDLGVHGG
                     SAIPLVTGDFEVARLNRRYTEENIDRLIFPVLIKSIRQYCEKVIVPVRDKTYYPILRE
                     AGVWAVQGEFRPMRFEQCEKLIG"
     gene            1014..1325
                     /locus_tag="KW011_003314"
     CDS             1014..1325
                     /locus_tag="KW011_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012125135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661577.1"
                     /translation="MSKDGYDPSITRGVQKFSHNGRWATVCYWGSALTEFHYGDRVFF
                     QNQYREYWLGTIERDCFVLTCETPLISVIDGLAYLSAEYRMNQSSEEGDWFCDQGELP
                     F"
     gene            1458..2126
                     /locus_tag="KW011_003315"
     CDS             1458..2126
                     /locus_tag="KW011_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661578.1"
                     /translation="MDCRKLNIVLLSTVAVLLVVVILVLIKSLFVGSEGFEWGSVSDW
                     VSSIANLIMAGAALYAAWNAKNWFQTKIKENALNQVTKFWTNCDLFSLEMLKTYENIC
                     NLNNTEPDIEPKNFAHLVKTEREVIDLLNIKLASLKSELQSLTFWKVSPKDEAIFNNY
                     FFSNQIALGTLREAISFDINDIHDRYHYNVSYGKLLVDTFDSLIEAHATLKKDMNNLF
                     VFSN"
     gene            complement(2383..2856)
                     /locus_tag="KW011_003316"
     CDS             complement(2383..2856)
                     /locus_tag="KW011_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006320261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661579.1"
                     /translation="MSITTIAATYKDVLTIAFSALSFIIACTSLYFSGRAAWHDRSRL
                     KITADLVYESFDEEPYKIEVIVINVGRRDAVLEGMLCHYEKGIRSHSSPKESIVIKEK
                     QRKKFELVSSDLVTIDNECDICHLEDITILDVEGNEHKIPNSHRLIALLKQDKKT"
     gene            complement(2939..3682)
                     /locus_tag="KW011_003317"
     CDS             complement(2939..3682)
                     /locus_tag="KW011_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBH7661580.1"
                     /translation="MNRKQFIQSHGATCSNWTWSWSFVNHDKKMVIFGAWDVQKEQER
                     SVILRERWKTKVVEGKERKNLGYIQAIEHIQLIAEGYELYTFDMEQGRNDENRDVAVI
                     KRFTPKLEKRYLRKEGTVWYADFLPNPFPDEITSPENYAEGAKKQVTVNSYERDPKAR
                     QACIDHHGTSCGCCEFNFEKVYGEHAKGFIHVHHIKPLHTVGEDYEVNPITDMVPLCP
                     NCHAMVHRGNEVLSVESLKALLFKDSNKI"
     gene            complement(4146..4607)
                     /locus_tag="KW011_003318"
     CDS             complement(4146..4607)
                     /locus_tag="KW011_003318"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661581.1"
                     /translation="MAKTYSTFDNRNIKALISQYKLYKDIPCEDIKNIMKDSVAKLKV
                     RDKISVYDVFAVLSNEDELRNDLLFNTINAIKVQQNKKQVKERMIRYYGAAITDAAKR
                     IEQYMLAHPNALPAPVYGQRTLTYKEFGKLLELQKQGDFIDDMIEYLKGLR"
     gene            complement(4729..5283)
                     /locus_tag="KW011_003319"
     CDS             complement(4729..5283)
                     /locus_tag="KW011_003319"
                     /inference="COORDINATES: protein motif:HMM:NF024784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBH7661582.1"
                     /translation="MNPKITWRLIPNSKHYYVSENGDVYNDKTKQYIATKNPSNGYKK
                     VSINGKSLYVHVLVCTLFNGAKPIGGRYTVDHIDRDKMNNKAKNLRWVTQKVNNSNRK
                     HKGTRTQKLTLKQWLMIVGEYLKGQLSQYAITKWFLNQTGINICPQNIGKLFKGTTYS
                     KWYTTYLTNKQRQRIQSLTIQRKP"
     gene            complement(5338..5640)
                     /locus_tag="KW011_003320"
     CDS             complement(5338..5640)
                     /locus_tag="KW011_003320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661583.1"
                     /translation="MKNDFDLVQYFDDYTIVGDMDYHYFYCVKYQEGSLEAHYERIGD
                     PEVLGTITWNGNYLIDGLPMVMFVLAAEDEHKECLNRLLIVCEKARDEYRLNATGL"
     gene            complement(5630..5815)
                     /locus_tag="KW011_003321"
     CDS             complement(5630..5815)
                     /locus_tag="KW011_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661584.1"
                     /translation="MKKVTLKERKHHVVTARLNDDQVLVLNQLISSGKVQKDSLSACV
                     QYLINQYMILQGGGNEE"
     gene            complement(6254..6628)
                     /locus_tag="KW011_003322"
     CDS             complement(6254..6628)
                     /locus_tag="KW011_003322"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661585.1"
                     /translation="MRKKENKAKKEKFRQFFPENYDIGISLNEENQLRLFSKRNDSQD
                     ESLCKYEFSNKIKVQYIFLPYSSEIQVITDEFPLTEAGCQECTQAFKHPISMELIEEI
                     KEAKCSVTGLVIYSKPILKLIS"
     gene            complement(6951..8885)
                     /locus_tag="KW011_003323"
     CDS             complement(6951..8885)
                     /locus_tag="KW011_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH7661586.1"
                     /translation="MLRPICYERVSSIQQVDMGKGLDDQRSSLTAYLDKNADKFTDER
                     IFITDAGLSAFKNANISPESQLGIFLQDVGNRKYGVGDALVVLSLDRLSRRSSWAEST
                     IQYIVNSGIEIHDISTNLVLRVDDPMSKIHMELIQHRSHNESLMKSVRAKLAWDNKIT
                     RAVQNGEVISNRMPNWLRNVDNKYQVIPEQAELIVKCFMWYREGLSTGEIVKRIGRKW
                     QMVTVSRLIRDRRLLGEHERYNGEVVPNVYPKIIDEELFLTANRMMDRVMLEKKKPAE
                     DLLLEPEVVREIFKLYETGLGSGAIVKRLPKGWSTVNVLRVLRDKKVVTMKIIDNLTF
                     ERANEKLSRNGIANRIRKDITIAQDDYITNLFPKMLKCGHCGGNIAIHYNHVRTKYVI
                     CRNREEKKICDAKSIQYMRIEKNILQLVKNVDFNKLMMEGKSDSDSLLASLREELSTL
                     QKEAETYQVKINERKAAGKKASLPLMTGLTEVQDRIEEIESEILNSKNIKEIPVFDFD
                     INKVLDPLNVELRAKLRKEVRLVLKSVKYWVFEKRIFVQLEYFNDVLSHTLVIDNKRG
                     GGEVLHEIVISETSGERVYTVNEEGKTVFIASVTSGTDMWSLAISRTKILDDVGLYLN
                     LLKREKFEIIVNEDMIDWMD"
     gene            8965..10899
                     /gene="yeaG"
                     /locus_tag="KW011_003324"
     CDS             8965..10899
                     /gene="yeaG"
                     /locus_tag="KW011_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HBH7661587.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMKGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            11012..12295
                     /locus_tag="KW011_003325"
     CDS             11012..12295
                     /locus_tag="KW011_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HBH7661588.1"
                     /translation="MTGFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKQPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            12442..13917
                     /locus_tag="KW011_003326"
     CDS             12442..13917
                     /locus_tag="KW011_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH7661589.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQKLQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
BASE COUNT         4017 a         3034 c         2704 g         4369 t
ORIGIN      
        1 atagggatgt agttcagcaa tgcgggaatc tcgtatgagg catccagtac ttgtattgtc
       61 atcatgatag atcttgttat agatgcgacc gcaatcactc tggaactgag gacggtagta
      121 cttcacccca ttacgtgttc ctgcatcaaa cagtactacg gccttgtcat ctacatagcc
      181 aacgtaagct ttatccatga agctgtgcgt agtagtacat ccagtactaa gggcaatcag
      241 taacccaatt agtaattttt tcattattgt tatcctatta atttttcaca ttgctcaaaa
      301 cgcattggcc tgaactctcc ctgtaccgcc catacaccgg cctctctgag aattgggtaa
      361 tacgttttat ctctgacggg tacgatcacc ttctcgcagt actggcgaat gctttttatg
      421 agtactggaa agatcagacg gtcaatgttc tcttctgtgt accttctatt taatcgggca
      481 acctcgaaat cacctgtaac cagaggaata gcagaaccac catgtacacc gagatcatca
      541 agccactgct ggttatgtac tttcatatcc cttgtcatct gccgaaggaa tggtaagtac
      601 tcgtatggtt caccacggcc agtagtaatc acacaataca aaccgttatc cctgaaccat
      661 gccgccttgc tttcaataag cattagttgc ccatgaagta cttcttcttc ccataggccg
      721 atctgcctga ttccctcgcg ttctacatgg ctgaccaact caacccccag caactgacca
      781 cccaaacttg ttatcggtat tgcccaatta tcaatgttca taactttgct cgttacgctt
      841 taagttattg ataattgatc atttttaaaa cattggcaat taaatcgatc aattttattg
      901 atcattacaa tagaattaat cattcaaagt ggtaaacaaa ggggtgctgg atttttggga
      961 aactgactgc aatactgtac atatatacag taatacatag aggagggtaa caaatgagca
     1021 aagacggcta tgatccatcc attacgcgag gggttcagaa gttctcacat aacggtcgct
     1081 gggcaaccgt gtgttattgg ggatctgcac taacagaatt tcattatggg gatagggtat
     1141 tcttccagaa tcagtacagg gaatactggt taggtactat agagagagat tgttttgtac
     1201 ttacctgtga aacaccacta ataagtgtta ttgatgggtt agcatatcta tctgctgagt
     1261 accggatgaa tcaaagcagt gaagaaggtg attggttctg tgatcagggg gaactaccct
     1321 tctgaggata cacgtactgc tcaccccaac aaaataacaa acccaacatg tgagcagtac
     1381 gtgaacgggt agtgtacttg attgtgtttc ggatggtaaa gtatttgccc ctttaatgcg
     1441 ttcaggaact actttcgatg gattgtagaa aattaaatat cgttctttta tcgaccgtag
     1501 cagtattact ggttgttgta attttagtac taataaaatc actctttgtt ggttctgaag
     1561 gtttcgagtg gggatcggtt agtgactggg ttagttctat tgccaatctc attatggctg
     1621 gagcagccct atatgcagca tggaacgcta aaaattggtt tcaaactaaa ataaaggaaa
     1681 atgctttaaa tcaagttact aagttctgga ctaattgcga tcttttttcg ttggagatgt
     1741 tgaaaacata tgaaaatata tgcaacctta acaatactga accagatata gaaccaaaaa
     1801 atttcgcaca tttagttaaa actgaacgag aagttatcga tctattaaac attaagttag
     1861 cctcattaaa aagcgaattg caatcactta ccttttggaa agtatcgcca aaagatgaag
     1921 ctatattcaa taattatttc ttcagtaatc aaattgcttt aggaacattg agggaagcga
     1981 taagttttga tataaatgac attcatgata gatatcatta caatgtcagc tatggaaagt
     2041 tattagtaga tacatttgat agtttaatag aagcacatgc aaccttgaaa aaggacatga
     2101 ataacttatt cgttttctct aattagagtc cacaactccc aatagacggt ggggtatcct
     2161 caccgctata actaacatct caacattaag atttataagg tcttcattaa agtactcccc
     2221 ctttcgtacc tcctatataa atagaggaag taatatatat caccatcgta ggtgttaaat
     2281 tccattctta tcatctcgta aaccctatag tgggttagta gttctaaccc tcaatctata
     2341 gtggcatcta acatttacct tacattccaa aaaatattat tcttaagtct ttttatcttg
     2401 cttaagtaat gcaatgagtc tgtgagagtt agggatttta tgttcattcc cctccacatc
     2461 taaaatagtt atatcttcta aatgacaaat atcacattca ttatctatcg tgactaagtc
     2521 tgagcttacc aattcaaatt tttttctttg cttttcttta ataacaatgc tctcttttgg
     2581 agatgaatgg ctcctaatgc ctttttcata atggcaaagc atcccttcca aaactgcatc
     2641 acgcctacca acattgatca ctatcacctc gattttataa ggttcctcat caaaagactc
     2701 gtagacgagg tcagctgtta tttttagcct tgatctatca tgccatgctg ccctaccgga
     2761 gaagtataag gaagtacatg caataataaa tgacaacgcc gaaaaagcaa tagttaacac
     2821 atctttatat gtagctgcta ttgttgttat gctcattttt ctctcaactt acatgtctaa
     2881 tttaaagcat attgtattca taacttttca agtatgtcgt atcaagtcta aaataaattc
     2941 aaattttatt gctgtcttta aacaacagtg cttttaaact ttcgacactt aatacttcgt
     3001 taccgcgatg taccattgcg tgacagtttg gacaaagtgg aaccatatca gtaattggat
     3061 ttacctcgta atcctcgcct actgtgtgta gcggtttgat atgatgaacg tgtataaagc
     3121 ccttggcgtg ttcaccatag actttctcaa agttaaactc acaacacccg caggaagtac
     3181 catgatggtc aatacaagcc tgtcgtgctt ttggatcacg ctcgtaggag tttactgtaa
     3241 cttgcttttt tgcaccctca gcgtagtttt caggtgaggt aatttcatct ggaaatggat
     3301 taggtaaaaa gtctgcatac caaactgttc cttctttccg aaggtaacgt ttttcaagtt
     3361 ttggagtgaa ccgtttgata acagctacat ctctattctc atcgtttcgt ccctgctcca
     3421 tatcaaacgt gtaaagctca taaccttcag ctatcaattg aatgtgttca atagcttgaa
     3481 tatatccaag attttttctt tcctttccct ccacaacttt ggttttccac cgctccctca
     3541 gaatgactga tctttcttgc tctttttgta catcccatgc accaaagata accattttct
     3601 tgtcgtggtt cacaaatgac caactccaag tccagttact acaagtagca ccatgcgact
     3661 ggatgaattg ttttctgttc atggtatccc tctcttaatt ccctctttgt acacaaaaaa
     3721 tgcctacttg atccagtact ttatgtagct atccaatagc atcatagtag tggaaatgac
     3781 cagagcgaga gttgtggaac tggcggggca tgtcgtactt accaattcac tttaggtact
     3841 tcatttgctg tactaacatc tatcgagcaa agcgagtagt aacgccttgc gggttttcga
     3901 gtactggcga gaaaaacgca acgttactaa ccaatactac gaggagttca cgacgagtag
     3961 tattcgtgac gcaagtcaca gtttcttgga atactgatag agaaagaaat acatggaatg
     4021 aaatggaatg gatttcgtat ctctatactt agttgccgta ttgatggtaa atagcgggtt
     4081 tcaggggctt tttttgccgt attacggcat acatccatgc cgtaatatgc cgtaataagt
     4141 tacaattaac gcaagccctt taagtactcg atcatgtcat caataaagtc accttgtttt
     4201 tgtagttcca atagtttacc aaactcttta tatgttagtg ttctttgtcc atatactggt
     4261 gctggcagtg cgtttgggtg tgccaacata tactgctcta ttcgttttgc tgcatccgtg
     4321 atcgctgccc catagtacct gatcatacgt tcttttactt gtttcttatt ctgttgtact
     4381 ttaatagcat tgatagtatt gaatagtaaa tcattccgta attcatcttc attactcaat
     4441 actgcaaaca catcatatac actaatctta tctctcactt taagtttagc tactgagtcc
     4501 ttcatgatgt tcttgatgtc ttcgcatggg atatctttat atagcttgta ctggctaatt
     4561 agtgctttta tgtttctgtt atcaaaagtt gaataggttt ttgccatgat aaataatcct
     4621 gtataaatta attaatcgtg acccgtagtg attacaggcc atttaataag tttggcttca
     4681 ttgccattga gttttgaata gatgccagta ctggaatact ggcatttttt atggtttacg
     4741 ttgaatggtt aggctttgta ttctttgtct ttgcttattc gtaagatatg ttgtgtacca
     4801 ttttgaatag gtagtacctt tgaataactt acctatattc tgagggcaaa tattaatacc
     4861 agtttgattt aaaaaccatt tagtgattgc atattgagat aattgtcctt taaggtactc
     4921 tccaacaatc attaaccatt gctttagagt aagcttttga gtacgagtac ctttatgctt
     4981 tctattggaa ttattaacct tctgagttac ccaacgtaaa ttttttgctt tgttattcat
     5041 tttatcgcgg tcgatatgat ctacagtgta tcttcctcca atgggtttag caccattgaa
     5101 taaagtacaa accaaaacat gtacatatag tgatttgcca ttaatagaaa cttttttgta
     5161 tccattagat gggtttttcg tagctatata ctgttttgtt ttatcattgt aaacatcgcc
     5221 attttcagat acgtagtaat gttttgaatt tggaattaat ctccaagtga ttttagggtt
     5281 catatcaaac gtccttgttg ttatcgcgtc actccatgtg atcgcgtctg ctactactta
     5341 gaggccagta gcatttagcc tatattcatc cctcgccttc tcacatacga ttagcaaacg
     5401 gtttaaacat tccttatgtt catcttcagc agccagtaca aacattacca ttggtaatcc
     5461 gtcgattagg taatttccat tccatgtaat agtacctaat acttctggat caccaattcg
     5521 ttcataatgt gcttctaatg aaccttcctg atacttcaca caatagaaat agtgataatc
     5581 catatcccct actattgtgt aatcatcaaa atattgtact aaatcaaagt cattcttcat
     5641 taccaccgcc ctgtaaaatc atgtactgat taattaagta ctgaacacag gctgataatg
     5701 aatctttctg taccttccca ctactaatta gctggttaag tacaagtact tgatcgtcat
     5761 taagacgtgc ggttactaca tgatgtttgc gttcctttaa agttactttt ttcatttcta
     5821 tatccttata ggtgtatttg tatactgtat ttataaatcc aaattaaaaa gatacctctc
     5881 tcgcaagaga ggcaatctta attaaaaagg aaatttattg tcgctatatt atttatgttc
     5941 aaagctggag cggaaggcaa aacgcattat cgttctgata ctattagtat agcaccaaaa
     6001 cttttagcgt ttcatttttc ttactacgta tttatttatc gatttgattt ttaatgggga
     6061 aagcaatagt actaaaatca tagataaaaa catgagcact atatatattg ggattatcag
     6121 aaagatgtaa agtacaaagc ctactatctc gtaaagtaat ttagtaaacc attccatttt
     6181 gaagtacccc cattcacatt caactttata gtatttagtg agggagtact taaaacgtaa
     6241 caatttggat gtgttagcta ataagtttta gtataggttt cgaataaatg acaagtccag
     6301 taactgaaca ttttgcttct tttatctctt caattagttc cattgatatt ggatgtttga
     6361 aagcttgagt acactcctga cagcctgcct ccgttagcgg aaactcatct gttataactt
     6421 gaatttcaga agaatacggc aaaaaaatgt actgaacttt aattttgttg gaaaattcgt
     6481 acttgcataa gctctcatct tgagaatcat tccgctttga aaaaagcctt aattgattct
     6541 cttcattcaa tgagatccca atatcataat tctcagggaa aaattgacgg aatttttctt
     6601 tttttgcttt attttctttc ttacgcattt tggcaatcct ctctttagaa ttcatctcat
     6661 ggtttcatcg ctcctattcg ttagtgaatg acgctatggt actggtttaa cgttgaaaag
     6721 tcaaaaaagt taattttacc cttgactatt ctaacacacg ctgatataaa tgatttgctg
     6781 tattttgcac ggaaatctta aacccatcgg gtgcgtagtt atatccaaaa ttcataatcc
     6841 ctcttttatt gctctatcta caatgccgtt agatttcgac atcgtagata tattgttcaa
     6901 atattaactc gcataattca ttctacaaga ccataaattc tcattagctt ttaatccatc
     6961 caatcaatca tatcttcatt aacaatgatc tcaaattttt cacgcttgag aagattaaga
     7021 tacaatccaa catcatcaag tattttggta cggctaatgg caagcgacca catatcagta
     7081 ccagatgtaa cggaggcaat gaaaacggtt ttgccctctt cattgacagt gtagacacgc
     7141 tcgccgcttg tttcagagat tacgatctca tgtagtactt cacccccgcc ccgcttgtta
     7201 tcaatcacaa gcgtatggga taggacatca ttgaagtatt ccaactgtac aaatatacgc
     7261 ttttcaaaaa cccagtactt aacagatttc agtactaatc ttacttcttt ccgtaacttt
     7321 gcacgtaact cgacattcaa tggatcaagt actttattaa tatcgaaatc aaatacaggg
     7381 atttctttga tgttctttga atttaatatc tcagattcta tttcttcaat tctatcctgt
     7441 acctcagtta aacccgtcat taatggtaac gaggctttct tacctgctgc cttacgttca
     7501 ttaattttaa cctgataggt ttctgcctct ttctgtaaag tacttaactc ttctcgcaat
     7561 gatgcaagca atgaatctga atccgactta ccttccatca tcaatttatt gaagtctaca
     7621 tttttgacaa gctgtagaat gttcttttca attctcatgt actggataga tttagcatcg
     7681 cagatcttct tttcttccct gttacgacaa ataacatact tagttcgtac atgattatag
     7741 tgaattgcaa tattgccccc acagtgaccg catttcagca tcttcgggaa gaggttagta
     7801 atgtaatcat cctgagcaat ggtaatgtcc ttacggatac gattagctat accattccgc
     7861 gataacttct cattagcccg ttcaaaggtg aggttatcga taatcttcat ggtaactact
     7921 ttcttatcac gtagtaccct cagtacattg acggtactcc accctttcgg caagcgtttt
     7981 acaatcgccc ctgaaccaag gcctgtctca taaagtttaa aaatctctcg gacaacttct
     8041 ggttcaagta atagatcctc tgctggcttc ttcttctcca acataacgcg atccatcatt
     8101 ctattcgctg tcaggaacaa ctcttcgtcg atgattttcg ggtaaacgtt tggtactacc
     8161 tcaccgttgt aacgttcatg ctcgcccaac aaacggcgat cacgtatcag cctggacaca
     8221 gtgaccatct gccactttct accgatacgt ttaacaatct cgccagtact caacccttcc
     8281 ctataccaca tgaaacattt aacaatcagt tctgcctgtt ccggtataac ttggtacttg
     8341 ttatctacat ttctcaacca attaggcata cggttggaga ttacttcccc gttctgtact
     8401 gcacgtgtga ttttgttatc ccatgccaat tttgcacgta ccgatttcat caatgactca
     8461 ttgtgtgaac ggtgctgtat cagttccatg tgaatttttg acataggatc atcaacccgt
     8521 aacacaaggt ttgtagagat atcgtgaatc tcaatcccac tattcacgat gtactggata
     8581 gtactttcgg cccagcttga tcgacgcgat agacgatcca gcgataaaac cacaagtgcg
     8641 tcacctacgc cgtacttgcg gttgcccaca tcttgcaaaa atatcccaag ctgagactct
     8701 ggagagatat tggcattttt gaatgcggaa agccccgcat cagtaatgaa aatcctttcg
     8761 tcagtgaact tatcagcatt tttatctaag tatgcagtca atgatgatcg ttggtcgtcc
     8821 aaacctttac ccatatcaac ttgctgaatg cttgatactc gctcataaca aataggacgg
     8881 agcatatttt ttatcccgtt gaagaattac gctaaaagtg ctgattcaca aatgttccca
     8941 gagtatacaa aaggacggca taccatgaat atattcgatc actatcgcca gcgatatgaa
     9001 gctgccaagg acgaagagtt cacactgcag gagtttctta ccacttgtcg gcaagatcgc
     9061 agtgcttatg ccaacgcggc tgagcggcta ttgatggcta tcggtgagcc tgtcatggtc
     9121 gatacagccc aggaacccag actttctcga ctcttttcta accgggtcat tgcacgttat
     9181 ccggcatttg aagagtttta cggcatggaa gacgcgattg aacagattgt ctcttatctg
     9241 aaacacgcgg ctcaggggct ggaagagaag aaacaaatcc tgtatctgct ggggcctgtg
     9301 ggtgggggta aatcatcgct tgctgagcga ctgaaatcat taatgcagct cgtaccgatt
     9361 tatgtattga gcgccaacgg tgagcgtagc ccggtcaacg atcatccgtt ctgtcttttc
     9421 aatccgcagg aagatgcgca gattctggaa aaagagtatg gcattcctcg ccgctatctc
     9481 ggcaccatca tgtcgccgtg ggcggcaaaa cgcctgcatg aatttggtgg cgatatcact
     9541 aagttccggg tagtgaaggt ctggccgtca attctgcaac aaattgctat cgccaaaaca
     9601 gaacccggtg atgagaacaa ccaggacatc tccgcgctgg tagggaaagt cgatattcgt
     9661 aaactcgaac actacgcgca gaatgacccg gacgcctacg gctattccgg tgcgctgtgc
     9721 cgcgccaatc aggggatcat ggaatttgtc gagatgttta aagcaccgat taaagtgctg
     9781 catccgctgt taaccgctac tcaggaaggt aactacaacg ggacggaagg tatctccgcc
     9841 ctgccgttca acgggattat tctcgcccac tcgaacgaat ccgaatgggt cactttccgt
     9901 aataacaaaa acaacgaagc cttcctcgac cgtgtttaca tcgtgaaggt gccgtattgc
     9961 ttacgcattt ccgaagagat caaaatctac gagaaattgc ttaatcacag tgaattgact
    10021 cacgccccat gcgcccctgg cacgctggaa acactgtcac gtttttccat tctttcgcgc
    10081 ctgaaagagc cagaaaactc cagcatttat tcaaagatgc gggtttatga tggcgaaagt
    10141 ctgaaagaca ccgatcccaa agccaagtcg tatcaggaat atcgtgacta cgccggtgtc
    10201 gatgaaggga tgaaaggtct gtcgacgcgt tttgcgttta agatcctctc ccgcgtgttc
    10261 aacttcgatc atgtagaagt ggcggcaaac ccggtccatc tgttctacgt cctagaacag
    10321 cagatcgagc gcgagcagtt cccgcaagag caggcagaac gctatctgga gttcctgaaa
    10381 ggttatctga tcccgaaata tgccgagttt atcggcaaag agatccagac ggcctacctt
    10441 gaatcctatt ccgaatatgg gcaaaacatt ttcgaccgtt atgttaccta cgcggatttc
    10501 tggattcagg atcaggagta tcgcgatccg gataccgggc agctgtttga ccgcgagtct
    10561 cttaacgccg agctggagaa aatcgagaaa ccggcgggga tcagtaatcc aaaagatttc
    10621 cgcaacgaga ttgttaactt cgtactgcgc gccagagcga ataacagcgg acgcaatccg
    10681 aactggacca gctatgaaaa actgcgcacg gttatcgaga agaaaatgtt ctccaatacc
    10741 gaggagctgt tgccggttat ctcgtttaac gccaaaacgt caaccgacga gcagaagaaa
    10801 cacgacgact ttgtcgaccg tatgatggaa aaaggctaca cccgtaaaca ggtgcgttta
    10861 ctgtgcgaat ggtatttgcg cgtacgtaaa tcgtcttaac aaccctggcc cggtacacat
    10921 gctaccgggc ctataacgac agcgaaccgt gggccagaga aatggcaaca caggcgtagc
    10981 atacagttgg caaatgtagt acggggggca tatgaccggg tttattgacc ggcgtctgaa
    11041 cggcaaaaac aaaagcatgg tgaatcgcca gcgtttttta cgccgttata aagcgcaaat
    11101 taaacagtcg atctccgagg ccattaataa gcgttcggtg actgacgtcg acagcggcga
    11161 atccgtatcc attcccacgg aagatattag cgaaccgatg tttcatcagg ggcgtggcgg
    11221 cctgcgccac cgcgtacatc cgggcaatga ccatttcgtc cagaacgacc gaattgaacg
    11281 tccccagggt ggcggcggag gttccggcag tggtcagggc caggccagcc aggatggtga
    11341 aggtcaggat gaatttgtct ttcagatttc gaaagatgag tatcttgatc tgctctttga
    11401 agatttggcc ttaccgaatc tgaaacaaaa ccaacaacgc cagctgaccg aatataaaac
    11461 gcatcgggcg ggttataccg ctaacggcgt tccagccaat atcagcgttg tgcgttcctt
    11521 gcagaactca ctggcgcgac gcacagccat gacggcgggc aagcgacggg aacttcatgc
    11581 gctggaagag aatttggcca tcatcagcaa cagtgaacct gcgcaactgc tggaagagga
    11641 acgtctgcgc aaagaaattg cagaattacg tgccaaaatt gaacgcgtcc cttttattga
    11701 caccttcgat ttacgttaca agaactacga gaagcagccc gatccctcca gccaggcagt
    11761 gatgttttgc ctgatggacg tttccggttc aatggatcaa tccactaaag atatggctaa
    11821 gcgtttttat attctgctgt atctgttcct cagcagaacg tataagaacg tggaagtcgt
    11881 atacatccgc catcataccc aggcgaaaga agtcgatgaa catgagtttt tctactcgca
    11941 ggaaacaggc ggcaccattg tttccagcgc cctgaaactg atggatgagg tagtgaaaga
    12001 gcgttataac ccggcacagt ggaatattta cgctgcacaa gcatcggacg gcgataactg
    12061 ggccgatgac tctccgcttt gccatgaaat cctggcgaaa aaattattac ctgttgttcg
    12121 ttattacagc tatatcgaaa ttacccgtcg tgcacatcag acattgtggc gagaatatga
    12181 gcatctgcaa tctactttcg acaactttgc gatgcagcac atccgcgacc aggatgatat
    12241 ttatccggtg ttccgtgaac tgtttcataa acaaaatgca acagctaaag actaaaacta
    12301 tcagccaggt cattatcgcc tggctgattt ttagcttact gtaaattatc tcatttatta
    12361 catacattag cttacaatcg ctttaaatat gacagcataa cctttacata atttagttcc
    12421 agaaaacaat cattcggaaa aatgattcag tcaacacgta tttccatggg gttattcttt
    12481 aaatattttt tatcgttaac gaaaattgat cctggtcaaa actatatatc tctgccatca
    12541 ataaaatcca gcactcacat tgctctcctt tttatggttt ctatgggtac acaaaaatta
    12601 aaagctcaaa gcttttttat tttcagttta ttgctgacgt taattttatt ttgcattact
    12661 accttatata acgaaaacac aaatgtaaaa ctcatcccac agatgaatta cctgatggtt
    12721 gttgtggctt tgtttttcct taacgccgtc atttttcttt tcatgttaat gaaatatttc
    12781 actaacaaac aaattttacc aacactcatt ttaagccttg catttttaag tggccttatc
    12841 tatttagttg aaaccattgt aattatccat aaaccaatta acggcagtac actgatccag
    12901 acaaagtcga atgatgtttc tattttctat attttccgcc aactcagttt tatttgttta
    12961 acctcgctgg cactcttttg ttatggaaaa gacaacatcc ttgacaacaa taagaaaaaa
    13021 acgggaatcc tgttgctggc gctgattcct tttttagttt ttccccttct ggcacacaat
    13081 ctgagcagtt ataacgctga ctattctttg tatgtcgtcg actactgtcc ggacaaccat
    13141 actgcgacct ggggaatcaa ctatacaaaa atattggttt gtctgtgggc atttttactg
    13201 ttctttatta tcatgcgtac acgattagcc agcgaactat ggccgttaat agcattatta
    13261 tgtctggcat cgctatgctg caacttactt ctactgactc tggatgagta taattacacc
    13321 atctggtata tcagtcgcgg gattgaagtt tccagtaaac tgtttgttgt gtcttttctg
    13381 atttataaca tttttcagaa actgcaactc tccagcaaac tggcagttca tgatgtgctg
    13441 accaatattt ataatcggcg ctactttttc aacagcgtag agtcattatt gtcgagacct
    13501 gttgttaagg acttctgtgt catgctggtt gatattaatc agttcaaacg catcaatgcc
    13561 caatggggac atcgtgtggg tgataaagtg ctggtttcaa ttgtcgatat tatccagcaa
    13621 agcatccgcc ccgatgatat tttagcgcga ctggagggtg aggtgtttgg cttgctattt
    13681 accgaactca atagtgccca ggcaaaaatc attgcggaac gtatgcgtaa aaatgtcgaa
    13741 ctcctgaccg gctttagtaa cagatatgat gttcctgaac aaatgaccat cagtattggc
    13801 acggtttttt caacgggtga cacgcgtaat atctcgcttg tcatgacgga agcagataaa
    13861 gccttacgcg aagcgaaaag cgaggggggc aacaaagtga ttattcatca tatttaagtg
    13921 caaaaatatt cagagccatg ctttttgcgt ggcttttgca tacaatttat tactattacc
    13981 cctaaatttc ccctccccag cggcgcagag atgagtataa ttagcgcccc tgtgccaggc
    14041 cgcaatcgaa ctttatctgg ttttctcgtt tcactaaccg aaggagtgcc atttatcatg
    14101 aaattgcacc atagaatgct ccgg
//
LOCUS       DAESHI010000110        14047 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.111, whole genome shotgun sequence.
ACCESSION   DAESHI010000110 DAESHI010000000
VERSION     DAESHI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14047
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.111"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            255..2021
                     /gene="adeD"
                     /locus_tag="KW011_003327"
     CDS             255..2021
                     /gene="adeD"
                     /locus_tag="KW011_003327"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBH7661590.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDVEKFAFTTLEVTE"
     gene            complement(2066..3457)
                     /gene="uhpT"
                     /locus_tag="KW011_003328"
     CDS             complement(2066..3457)
                     /gene="uhpT"
                     /locus_tag="KW011_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HBH7661591.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3595..4914)
                     /gene="uhpC"
                     /locus_tag="KW011_003329"
     CDS             complement(3595..4914)
                     /gene="uhpC"
                     /locus_tag="KW011_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HBH7661592.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4924..6426)
                     /gene="uhpB"
                     /locus_tag="KW011_003330"
     CDS             complement(4924..6426)
                     /gene="uhpB"
                     /locus_tag="KW011_003330"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HBH7661593.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6426..7016)
                     /gene="uhpA"
                     /locus_tag="KW011_003331"
     CDS             complement(6426..7016)
                     /gene="uhpA"
                     /locus_tag="KW011_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HBH7661594.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(7091..7381)
                     /gene="ilvN"
                     /locus_tag="KW011_003332"
     CDS             complement(7091..7381)
                     /gene="ilvN"
                     /locus_tag="KW011_003332"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBH7661595.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7385..9073)
                     /gene="ilvB"
                     /locus_tag="KW011_003333"
     CDS             complement(7385..9073)
                     /gene="ilvB"
                     /locus_tag="KW011_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HBH7661596.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVEE"
     gene            complement(9179..9277)
                     /gene="ivbL"
                     /locus_tag="KW011_003334"
     CDS             complement(9179..9277)
                     /gene="ivbL"
                     /locus_tag="KW011_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HBH7661597.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9842..9931
                     /gene="tisB"
                     /locus_tag="KW011_003335"
     CDS             9842..9931
                     /gene="tisB"
                     /locus_tag="KW011_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HBH7661598.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            10211..11395
                     /gene="emrD"
                     /locus_tag="KW011_003336"
     CDS             10211..11395
                     /gene="emrD"
                     /locus_tag="KW011_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HBH7661599.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11403..11900)
                     /locus_tag="KW011_003337"
     CDS             complement(11403..11900)
                     /locus_tag="KW011_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH7661600.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11897..12259)
                     /locus_tag="KW011_003338"
     CDS             complement(11897..12259)
                     /locus_tag="KW011_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HBH7661601.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12249..12596)
                     /locus_tag="KW011_003339"
     CDS             complement(12249..12596)
                     /locus_tag="KW011_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HBH7661602.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12705..13154
                     /locus_tag="KW011_003340"
     CDS             12705..13154
                     /locus_tag="KW011_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661603.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13403..>14047)
                     /locus_tag="KW011_003341"
     CDS             complement(13403..>14047)
                     /locus_tag="KW011_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH7661604.1"
                     /translation="LATLSDGTVFEPVNSFQKNQKKLARLQRQLSRKVKFSNNWQKQK
                     RKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSLPGRNVR
                     AKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPAYTSQRCVCCGHTAKENRLSQ
                     SKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         3318 a         3880 c         3644 g         3205 t
ORIGIN      
        1 gtcccatgct gaggtagttt aaataatcgc gaaagcgtcc ctgattcatt actcacgtaa
       61 tcggtattgt cattattcat ttttttgtcc attggaggag atttaatccc ttgcttttgt
      121 aaaacaaatg acaacaacgg ttcagtgata attatcacat ttcaattgca cattaatgga
      181 tattctttaa taatctcgcg acgtttcttt atgataaata ataatcaaat tgataaaatc
      241 aaaatgagaa aaatatgaat aattctatta accataaatt tcatcacatt agccgggctg
      301 aataccagga attgttagcc gtttcccgtg gcgacgctgt tgccgattat attattgata
      361 atgtctctat tctcgacctg atcaatggcg gagaaatttc cggcccaatt gtgattaaag
      421 gacgttacat tgccggtgtt ggcgcagaat acgctgatgc tccggctttg cagcggattg
      481 atgctcgcgg cgcaacggcg gtgccagggt ttattgatgc tcacctgcat attgaatcca
      541 gcatgatgac gccggtcact tttgaaaccg ctaccctgcc gcgcggcctg acgaccgtta
      601 tttgcgaccc tcatgaaatc gtcaacgtga tgggagaagc cggattcgcc tggtttgccc
      661 gctgtgccga acaggcaagg caaaaccagt acttacaggt cagctcttgc gtacccgccc
      721 tggaaggctg cgatgttaac ggtgccagtt ttacccttga acagatgctc gcctggcggg
      781 accatccgca ggttaccggc cttgcagaaa tgatggacta ccctggcgta attagcgggc
      841 agaatgcgct gctcgataaa ctggatgcat ttcgccacct gacgctggac ggtcactgcc
      901 cgggtttggg tggtaaagaa cttaacgcct atattactgc gggtattgaa aactgccacg
      961 aaagttatca gctggaagaa ggacgccgga aattacaact cggcatgtcg ttgatgatcc
     1021 gcgaagggtc cgctgcccgc aatctcaacg cgctggcacc gttgatcaac gaatttaaca
     1081 gcccgcaatg catgctctgt accgatgacc gtaacccgtg ggagatcgcc catgaaggac
     1141 acatcgatgc cttaattcgc cgcctgatcg aacaacacaa tgtgccgctg catgtggcat
     1201 atcgcgtcgc cagctggtcg acggcgcgcc actttggtct gaatcacctc ggcttactgg
     1261 cacccggcaa gcaggccgat atcgtcctgt tgagcgatgc gcgtaaggtc acggtgcagc
     1321 aggtactggt gaaaggcgag ccgattgatg cgcaaacctt acaggcggaa gagtcggcga
     1381 gactggcaca atccgctccg ccatatggca acaccattgc ccgccagcca gtttccgcca
     1441 gcgactttgc cctgcaattt acgcccggaa aacgctatcg ggtcattgac gtcatccata
     1501 acgaattgat tacgcactcc cactccagcg tctacagcga aaatggtttt gatcgcgatg
     1561 atgtgtgctt tattgccgta cttgagcgtt acgggcaacg gctggctccg gcttgtggtt
     1621 tgcttggcgg ctttggactg aatgaaggtg cgctggctgc gacggtcagc catgacagcc
     1681 ataatattgt ggtgatcggt cgcagtgccg aagagatggc gctggcggtc aatcaggtga
     1741 ttcaggatgg cggcgggctg tgcgtggtac gtaacggcca ggtccaaagt catctgccgt
     1801 tgcccattgc cggcctgatg agcaccgaca cggcgcagtc gctggcggaa caaattgacg
     1861 ccttgaaagc cgccgcccgt gaatgcggtc cgttacccga tgagccgttt attcagatgg
     1921 cgtttctttc tctgccagtg atccccgcgc taaaactaac cagtcagggg ctatttgatg
     1981 tcgagaagtt tgccttcact acgctggaag tcacggaata ataaaaaagc ccggcgtcat
     2041 gccgggcaaa agtcaccagt tacgtttatg ccactgtcaa ctgctgaatt tttttctcgc
     2101 ggcggatttt gcgttcttcc atcaccgcca ctatcgccat caggcagata caaccaatcg
     2161 cggcgatatc aagtgcggcg aaggtgcctg cccagccggt aaggccgaat accggcgtcc
     2221 catcggcaat cattcccaga cctaacttgg caaagctgtc accaatcagg taagcaaagg
     2281 tgcctttaat accatcggca gcgccaatcg cttttttagg tacaaagcca acagcagcca
     2341 caccaatcaa caattgcggg ccaaagacca ggaaacccaa cgcaaagaga gaagccagat
     2401 agatatattc gttactggca tgttgataca caccgagcgt ggcgataatc agcgccagcg
     2461 cgatgcaggc caccaggcca cggcgaccgt tcgctaggtc agagagccag ccccacagca
     2521 gcgtaccgac cagcgcacca gcttcaaaca gcgtaaagcc ctgaatcgcc accgctttag
     2581 agagtttcag ttcctggaac gcgtatacgg ttgaccactg gtcgatacca atacgtacca
     2641 catagaggaa aatgttggcg aagcacagca gccagatcac tttgtttttc agcacatact
     2701 caacaaagat ctgccacttg gtcatatcgg tagattctgt ctctttgtcc tcttcgctga
     2761 tctcctcgcc gaacagttct tcagctttgc cgaggccata agattccggg gagtcgctgc
     2821 cgtaacgcag gccgataaaa ccgacaatca gcgcgataat cgacgggaag ataaacatgc
     2881 cgatgacatg gccatcgaac aggtaatttg ccccgaacag cgccacgcct gctgcgcctg
     2941 caccgccaag gttgtgagaa atattccaga aaccgaggaa tgtcccgcgt ttacgacgcg
     3001 gcgtccattt ggtgatggtg gagtaactgc acgaaccgcc ggtactctgg aaaaagccgc
     3061 ttaaggcgta gaaggcaatc atcaggaaca ggctaaccga gccgctgccc atactggcac
     3121 tgaagcccag catacaaatg gcagacagga tcagcatgaa cggcaggaat tgtttggtgt
     3181 ttttgccgtc ggcgtagtag gaaaccagcg ttttacccac gccataagtg atggagaaac
     3241 ccaggccgat catccccagc tgcgtcatgc tcaacccgta ggtcgagatc atgtcgttct
     3301 gcgcgatgtt aaagttcttg cgaatcaggt acatcgtcag gtagccgata aagaccacca
     3361 ggtaggattg catgaacggt ttgaaccaca ttttgcgccg cacttcgagc ggaaggtcca
     3421 gggtcggctt gcgaacctgg tttaagaaag ccagcatggg ttactcctga aatgaatacc
     3481 tgcgtgaggc atgcattgta aaaatcagca gcaaatcagg cctgagatag cgtccaggca
     3541 aaacctggaa aatttcttag ttttgccgga tatgaagtga aaaggtgaga tgcatcacgc
     3601 ttcgcgcggt gtctgggcgt tcaaaaaggg cagtaacagc agtgcggaaa tcccggcggc
     3661 gatagagata accacaaaaa atccgctcca gtgccaggta tcgagtactt tcgccagcgg
     3721 ccaaccagca agcgacgccc ccagataagc aaacaagccg acaaaccccg tcgccgcccc
     3781 tgccgcctct ttgtgggaac actctgccgc cgccataccg attaacatct gtgggccaaa
     3841 gacaaaaaaa ccaatggtga agaagcaggt tgcctgcatc acgtagctgg caaatggcat
     3901 cagccacagg gagccgactg aaagcaaaat tccggcggcg aaaatcaaat tcatcggccc
     3961 tcggttgccg ttaaacaatt tgtccgagcc ccaacccgct accagcgcac cgataaatcc
     4021 acccagttca aacatcgtca ctgccgtatt cgccgtgacc agatcgacgc ccagcgtctc
     4081 ggacatatac aaattgcccc agtcgttgat cgccgcccgg accacataga ccagcacata
     4141 gcaaaacgaa agcagccaga tatacggatt cagcaacaca tatttggtga ggatctcttt
     4201 acgcgtcagc cctgcccctt cttgttgttg agctatctcc agcgcgtcgt gtcgccattc
     4261 accgaccgcc ggtaaaccta acgcctgcgg gcgatcgcgc agccgccagc agagaaaaat
     4321 tcccacgacg atcgccatac aaccagcaat catcatcccg gcacgccagc cgtaatgtag
     4381 tgcagccgct gccatcacaa tgggaatgag tgcgccgccg acgttatgcg ccgtgttcca
     4441 taatgcccac caaccaccgc gctcggtacg tgaataccag gccgttaaca gacgcgcaca
     4501 caccggtgaa ccccagccct ggaaaaaggc gttcagcacc cagagtacgg caaacgccca
     4561 tagcgacgtc gagaagccaa acagaatgtt gataatgccc gtggcgataa gccctatccc
     4621 cataaaataa cgggcatttg agcgatcgct gacaatgccg gagacaaact tcgacacgcc
     4681 ataggtaatg taaaacaggg tcgctaacag gccgatatcg ctacggctga gcacgccgtt
     4741 agcaaggatt tctggtacgg cggcgttaaa acttttccgc gtgaagtaaa acagcgcgta
     4801 acccagccag atggtcagca gaatatgccg acgccagtag cgatagcggg catcaatttc
     4861 atatttatca gccattaatg gcgcatcggc aggcgctttc agaaacggca acatcatcaa
     4921 accttagaca tagcgttgag gtagagaaac gctgacacgc gtgccgtgca gacaggaaat
     4981 gtgtaatgtg ccacccagcg ccgttacgcg ctcgcgcatt ccggtgaggc caaaaccttg
     5041 ttgcccggaa cccggcggca aaccgctgcc atcgtcttca ataaccagca tcaaccgttc
     5101 atcctgctgc cagccttgca gggtgaccgc gctggcatca gcatgtttca caatgttgtt
     5161 cagcccttcc tggcagacac gaaacagcgt cacgcgctgg ttttcgctta acgctgattc
     5221 atcgattcgc cattcgagat ggctgacaat accgcgccct tccagctcca tttcccgcat
     5281 cagtgagcgg atggcctgct ccagggtgag atcatccaac tggcgcggac gtaaccgacc
     5341 caacaaacgg cgcaccgcgt cgtaaacgcc cagcgatagt tgttcgatga gttgcccgct
     5401 ctgcttcacg ctggcgttat ctgccgccag ccgctgaaca atgcccgcct gagtacgaat
     5461 agcagtgatg gtctggccga tatcatcatg cagctcacgc gccacatcac ggcgcacgct
     5521 ctcttcggtt tccagcaacc gttcagccag atgctgattg cgcgccagtt ccttttgcag
     5581 cgactggtta agttcacgca accgctggat gccagcgcca agcaacaacc ctgtcagact
     5641 ttgcaccagc agcgagagca ataaatccac cggatgatcg cgccaggttt gactggcgat
     5701 cagcgcgatg gcgttcatca acgtcgcaat cagcgcccct tgccaaccat agtgccaggc
     5761 cagcgcgata atcggcagca ccagacagaa tggcgtaaag cgcgacagtt cgtccggcaa
     5821 tcccaactgg agccagagac tgataacaaa cagcagcaag taccagacca gatgtcgccc
     5881 gcgccagttg attggctgag aaaccagtga cgggccgagc ggcagccagg tgttattggc
     5941 gagatagtgc cagaacacca gacatatcgg ggccagcgtc aggccgccag ttaaagtcag
     6001 caacagcgca ttccacgact ctttgccgtg ccaaagccag ggcagcgact gcaacaacgc
     6061 cgccgccgtt aacgccgccc cctgtaacag caaggtgcgc cagtcacgct gatggcgata
     6121 gcgcgagatc agcgctacgg gcagtaacgt cagtaaacta ccgatcatca ataacggaaa
     6181 atgggttaaa ccgaccgcct gcgttagcca gtaaatcagc agccactccg cacccagcaa
     6241 tacgggccag tatccgcgcg ggcattgcag cattagcccc agacgcagac caaacggaaa
     6301 taacagcacc gccatatcag ggcgctcaac cagatgcagg ctgatactcc acaggcaaaa
     6361 ccatgcggca gagaagataa aaaagcaggc aataacggta attaagcggg agaacaacgt
     6421 cttcatcacc agccatcaaa catgcggcgc gccagctcta cgtcgttact gacgcccagt
     6481 ttttccatca gattggcgcg atggacgtgt accgttttcg gtgacaagcc cagttcggcg
     6541 gcaatctctt tcaccgccat tccttgcgcc agtttttccg ccacctggcg ttcacgtttg
     6601 gttagcgggt cctgacgacc ggatgccagt ttaatggcaa tatccggcgt cagataacag
     6661 ccacccgtgg caaccgtatg caccgcagca atgagttcat ccgggctaca gcgtttggaa
     6721 agaaagccgc gtgcccccgc gttaagcgcc tgctcaacca gcgcaggact gtcgtgaacg
     6781 gagagcataa tcgtcgccat acctttcggc agctggctta gcagctccag accggagata
     6841 tcgggcatgg agatatcgca aatacacacc tgcacaccgc gccccggcag ccccgccagc
     6901 gcctcgcgcc ccgaaccaaa ctcggcaact acctgcaaat caggttccag ccccagcagc
     6961 tgcgcaaagc cggagcggac gatgaggtga tcgtctataa gggcaacggt gatcatggtc
     7021 ttgtcctggc gggtaaaaaa aaacgcgctt accttaacga taagcgcgat gttgttcaag
     7081 ccttgagcgg ttactgaaaa aacaccgcga tcttgttaaa catcgtcgga tcggactgat
     7141 tacgctgcac tttcacgaca tcttccagct tatcgatttg gcttatcatc tgctccagac
     7201 gctggtcgtc attgaccagt agccagatat ggcttttgtc gctgtcctga atcggcagac
     7261 aaagaatgcc ttcaacgtta aaagcgcggc gggcaaaaag gccacaaacg tgggtcatta
     7321 cgcccggatg gttgcgaacg gtgagctcca gaattacgtt gtcatgagtt gtgttttgca
     7381 tggcttattc ctccaccatt tcagtattcg ccgcacctgg cggcaccatc gggtaaactt
     7441 tttcttcggc atcaatgcgc acatggatca gcgccgggct agggcgattg atgatttcct
     7501 gcaatgcagc ctgcggatcg gcttcgttat tcaaatcaca ggtttcgagg ccgaatccgg
     7561 cggcaatctg cataaagttg attttgcccg gataggtggc ggcaaaaacg ccttgctcgt
     7621 agaacagact ctgttgctga tgcaccagcc ccagcgcttc gttgttcatc agaatgattt
     7681 tgacatccag ctgattttca ctggcggtcg ccatctcctg aatattcatc atcaggctgc
     7741 cgtcgccgga gaaacacaac actttgcgat ccgggttcgc cagcgccgcg ccaatcgccg
     7801 caggcaggcc aaaacccatc gtgcccagcc caccggaggt cagccactgg cgtgggcgat
     7861 tgagcggata agcttgcgcg gtccacatct gatgctgacc cacatcggtg gtgataatcg
     7921 cattgtcatc gacacaggcg gcaacggcgt tgatcaggcc gtaatggctt aatggatcgc
     7981 acgctttcgg gattggacac gggaattcgc gctgcaaatc cgctaccaac tggtgccact
     8041 ctgcacgcgg ttgcgcttcc acctgcggga tcaattgcgc cagcacgtca tcaacatccg
     8101 cctgaatcgc cacatgcggc tgcttgattt tacccagctc tgcgcgatcg atatcgacat
     8161 gaataatttt ggcattcgga cagaactgct cggttttgcc aatcgcccgg tcatcaaaac
     8221 gcgcaccgag cacaatcaac aaatccgcct cctgcaaaat atagttggta ctgcgcacgc
     8281 cgtgcatccc cagcataccc agcgacaacg gatgcgcttt tggcagcatg cccagcgcca
     8341 ttaaagtcat ggtggtaggc agttgcgctt tctccgccag ttcacgcacc cgcgcaggcg
     8401 cattgatcac accaccaccc agataaagca ccgggcgttt ggcagcgtta atcattgtag
     8461 ctacgtcacg aatgctttct tcgctaaagg cgggtgcagc ggctttttct gccaccgcgg
     8521 gctgagcttc aatctcaaaa accgccgttt gtacatcctt aggaatgtct atccacaccg
     8581 ggccagggcg gcctgattgc gcaatgcgga aggcatcgct catgacctgc gggagttctt
     8641 cgatatgtct gaccagatag ttgtgtttgg tgatggggat agagatgccg taggtgtcga
     8701 cttcctggaa ggcgtcggtg ccgatcatcg aggcgggaac ctgaccagtg atgcaaatca
     8761 gcgggatgga gtccagccgc gcatcggcaa tggcggtcac caggttagtc gcacccggtc
     8821 cgctacaggc catacagacc gccggtttac cgtcggtgcg cgccattccc tgagcgataa
     8881 agcccgcgcc ctgttcatga cgggccagaa tatggcggat ttgcgtgctt tggcttaagg
     8941 catcgtaaac aggcaggata gaaccgcccg gaatgcctgt cacaatctta atgccctgct
     9001 gttccaggaa atgaacgata aattctgcgc cggtaaagcg cttacgcgtc aatgttgtgc
     9061 ccgaacttgc catgctccag tccttttctt ctgggccgac tttccgggag gtgcttaaac
     9121 gaaaaacccc gcccggtttg cgccgacggg gttttggaat cgtgtgttgt tccagtccct
     9181 acggcgcatt gccgacgacc accaccacac gcacgacgac cactgcggcg gatggcgcag
     9241 ttggtagtag ttttgcgttg aacatggaag tagtcattgg ggaccttgtt ggttttgtgt
     9301 ttaacaatat ttatacaagc acagctttac aggggagaca atggaaaatt tttcagcaag
     9361 ggaaaattga ggggttgatc acgttttgta ctgaattgca gataacaaaa aaccccgccg
     9421 gagcgaggtt tcgtcagtcg cccgcggctg gtaaccgcaa agcacactgt attatgtcaa
     9481 cacagaaagt atacgtgttc cgcgcagaac gcgcaatttc ggcacgaatt ttgacgtatt
     9541 tagtgcatag ttgagtatcg atcacagttt gcgttttgtc caaatattac tgtttattta
     9601 tacagtaaac ttctataata tcactgtacg caatgtgtta tgcgggggcc gcatcgttac
     9661 ccggcgcact aagtcctggc tgaaacgggt ggtgccgtca gcgccttaac cccgcgtgag
     9721 cacactgtgt tatgtcaaca agcacaaagt ttctccttga gataccgcgt gcacaacagc
     9781 tggcaacagg cagcggaaag gtacgtcagc tggcagtgct cctgaaccac aggagacgcg
     9841 tatgagcctg gtggatatcg ccattcttat cctcaaactc attgttgcag cactgcaact
     9901 gcttgatgct gttctgaaat acctgaagta attcagattc aagtcgcacc aaaggggagc
     9961 gggtaaccgc tcccctttta tatttagcgt gcgggttggt gtcggatgcg atgctgacgt
    10021 atcttatccg ccctaccatc tctcccggca acatttattg ccgcttttgt ttacatattc
    10081 tgccgctaaa caactcccca ttcctggcgt atatctggct aacattcatc aatgtgatag
    10141 attcctctcc cgcatttatg ggaatgcgta gtgacttact ctaattattt ttataaaagc
    10201 atccgtgata atgaaaaggc atagaaacgt caatttgtta ttgatgttgg tattactcgt
    10261 ggccgtcggt cagatggcgc aaaccattta tattccagct attgccgata tggcgcgcga
    10321 tctcaacgtc cgtgaagggg cggtgcagag cgtaatgggc gcttatctgc tgacttacgg
    10381 tgtctcacag ctgttttatg gcccgatttc cgaccgtgtg ggtcgccgac cggtgatcct
    10441 cgtcggaatg tccattttta tgctggcaac gctggtcgcg gtcacgacct ccagtttgac
    10501 ggtattgatt gccgccagcg cgatgcaggg gatgggcacc ggcgttggcg gcgtaatggc
    10561 gcgtactttg ccgcgagatt tatatgaacg gacacagttg cgccatgcta acagcctgtt
    10621 aaacatgggg attctcgtca gtccgttgct cgcaccgcta atcggcggtc tgctggatac
    10681 gatgtggaac tggcgcgcct gttatctctt tttgttggtt ctttgtgctg gtgtgacctt
    10741 cagtatggcc cgctggatgc cggaaacgcg tccggtcgat gcaccgcgca cgcgcctgct
    10801 taccagttat aaaacgcttt tcggtaacag cggttttaac tgttatttgc tgatgctgat
    10861 tggcggtctg gccgggattg ccgcctttga agcctgctcc ggcgtgctga tgggcgcggt
    10921 gttagggctg agcagtatga cggtcagtat tttgtttatt ctgccgattc cggcagcgtt
    10981 ttttggcgca tggtttgccg gacgtcccaa taaacgcttc tccacgttaa tgtggcagtc
    11041 ggttatctgc tgcctgctgg ctggcttgct gatgtggatc cccgactggt ttggcgtgat
    11101 gaatgtctgg acgctgctcg ttcccgccgc gctgttcttt ttcggtgccg ggatgctgtt
    11161 tcctctggcg accagcggcg cgatggagcc gttccccttc ctggcgggca cggctggcgc
    11221 gctggtcggc ggtctgcaaa acattggttc cggcgtgctg gcgtcgctct ctgcgatgtt
    11281 gccgcaaacc ggtcagggca gcctggggtt gttgatgacc ttaatgggat tgttgatcgt
    11341 gctgtgctgg ctaccgctgg cgacgcggat gtcgcatcag gggcagcccg tttaagcgca
    11401 cgtcaccgca gcatcgtcat cagctccatg ggagaacgat gctgctttat cagatcacgc
    11461 atcacccgca tatgcggtgc ggagtaagaa taaaacgcct gatagcccgc acaaagcacg
    11521 ctgtctggcg tctcctgccg agcgtgcggg cacaggcgat gccagtcgca cgcctggcag
    11581 tcacagcgcg gttgcgggtt ttcgctgcca caccattttt gtaaggtggc gtcaaaaagc
    11641 tcaatcgaga tacgactgat atctgcccgc acccaacact cgaacagggc aattaaaaat
    11701 ttcccccaca gctctgctgt caccgaagcg gccgtcagat cgccccggtc atcaaattca
    11761 agatagggaa tgagctgtac ggagcggatg ccccgctgtt tgaaattgtg ataaaaatcg
    11821 gcaggttgca gagcatcctt gtactctaca accagtttat gcctgtcctc ttcagcatca
    11881 atgacctgac ttcctgtcat gcgactctct caataaatac gataagttga tgtatatgcg
    11941 ttacagcaaa cagtattgcg agagataaca ccgcgaggga gatcaaaaat ttgtcacgga
    12001 ctacgtgaaa ttgggaaaaa tcgctattcg tgacatccat taaattgcga ttacgggtgt
    12061 agtgccagag gatcagcgcc acgatggcga gaatgccaat cgaaatccag aataacatgc
    12121 ccgcctggtg ccagttgtgt ttgatagcca acgccatcaa cgcgccgtag cccagcatgg
    12181 tacgaaacca cgccagtgat gtgcgctccg gctgcagtcc tgggtcggca atgcgtctgg
    12241 ctttgcggct atccggcata caacaccagt cccataacga tcaccgccac gaccatcaat
    12301 attaagctaa tgattaataa gctgttggtg tagggcaggt cctctttcag acgcatcgct
    12361 ttttcattgc gtaaccagcg caggtagcca tacattgcta acccgccgga aaacagacac
    12421 aacaacaacg ccagcagttc acgaatgacg ggcgtggcga aatctggcgc aagttgatca
    12481 agcccaaccc ccgccgccag aaatcctagt gcggtacgga tccacgccag aaaagtacgc
    12541 tcattagcca gcgagaagcg gtaatcgggc gcttctccaa ggcgggaaat tttcattgtt
    12601 gctcctttgc aatttcacta actctctacc tttagcggtt ttttttaaga atttcgtccg
    12661 cccgtttcct gatatgcttt tgccatccgc tacaaggagg tcagatgggt atcatagcgc
    12721 aaaataaaat cagttcattg ggcatgctgt tcggtgccat cgctttaatg atggggatta
    12781 tccacttttc attcggccct ttttccgcac caccgccaac gcttgaaagc atcgtggcgg
    12841 acaaaacggc tgaaattaaa agaggtcttc tcgccggaat aaaaggcgaa aaaattacca
    12901 ccgtggagaa aaaagaggac gtagatgtcg ataaaatcct cgatcaaagc ggtatcgcac
    12961 tggccattgc tgcgctactt tgcgcatttg ttggcggtat gcgtaaggaa aatcgctggg
    13021 gcattcgcgg cgcgctggtt tttggcggcg gaacgctggc tttccatacc ttgctttttg
    13081 gtatcggcat cgtgtgcagc attttgctga tattccttat cttttccttc ctgaccggcg
    13141 gttcgctggt ttaagcagcc tgaataatgc ccgccgggtg atcatcagcg ggcattatcg
    13201 tcagaacttc tgttttttct cttccacctt caccccttgt gtcggtaggc cggtgtcata
    13261 gtcacgtacc accggcgaat agccatcttg tgggcgtgga cgaaatgccc ccatccagcg
    13321 cggccgtgca tctttacgcc acggacgcag gctccattgt tgacatcctc cacgccctga
    13381 aggacgtgga cccctgctac gttcaggctg tcgcctgaat catttcggtg ggttcctgct
    13441 tcaacgagcg gcctgactgc accatctctc cacaggcaag aacggcgtgc cccgccgcta
    13501 aaatgttacg agcgccattt acatcggcgt tcgctgtata tccacatacc tggcatctga
    13561 atttactttg tgacaggcga ttttctttcg ctgtatgacc acagcacacg caacgctggc
    13621 ttgtgtacgc tggcggaaca gcaagcacct gaccaccacg ccagagctgc ttgtacgcaa
    13681 gctggcggcg catttcatac cagccctgat ccagtatcga acggtttaaa cctgattttg
    13741 cccggacatt gcgccccggc aggctgaccg tacccgctgc tgactttgac atgttgctga
    13801 ccttcaaatc ctcaatgact atcattgcgt ggtttttgct gacggtcgtt gtgactttgt
    13861 gaaggtagtc cctgcggata tttgcgatac aggaatgcag tcgctgtatt ttgcgtttct
    13921 gcttctgcca gttgttgctg aatttgacct tgcggcttaa ctgtcgctga agtctcgcca
    13981 gcttcttctg gtttttctga aaactgttta caggctcaaa gactgtgcca tctgacagcg
    14041 tggcgag
//
LOCUS       DAESHI010000111        14001 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.112, whole genome shotgun sequence.
ACCESSION   DAESHI010000111 DAESHI010000000
VERSION     DAESHI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14001
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.112"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            75..2216
                     /gene="ldcC"
                     /locus_tag="KW011_003342"
     CDS             75..2216
                     /gene="ldcC"
                     /locus_tag="KW011_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HBH7661605.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2273..2662
                     /locus_tag="KW011_003343"
     CDS             2273..2662
                     /locus_tag="KW011_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH7661606.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2727..4025
                     /gene="tilS"
                     /locus_tag="KW011_003344"
     CDS             2727..4025
                     /gene="tilS"
                     /locus_tag="KW011_003344"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBH7661607.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4074..4334)
                     /gene="rof"
                     /locus_tag="KW011_003345"
     CDS             complement(4074..4334)
                     /gene="rof"
                     /locus_tag="KW011_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HBH7661608.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4321..4521)
                     /locus_tag="KW011_003346"
     CDS             complement(4321..4521)
                     /locus_tag="KW011_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HBH7661609.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4687..5232
                     /locus_tag="KW011_003347"
     CDS             4687..5232
                     /locus_tag="KW011_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HBH7661610.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5229..5590
                     /gene="arfB"
                     /locus_tag="KW011_003348"
                     /pseudo
     CDS             5229..5590
                     /gene="arfB"
                     /locus_tag="KW011_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
     gene            5664..6374
                     /gene="nlpE"
                     /locus_tag="KW011_003349"
                     /gene_synonym="cutF"
     CDS             5664..6374
                     /gene="nlpE"
                     /locus_tag="KW011_003349"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HBH7661611.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6529..7353)
                     /locus_tag="KW011_003350"
     CDS             complement(6529..7353)
                     /locus_tag="KW011_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HBH7661612.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7406..9124)
                     /gene="proS"
                     /locus_tag="KW011_003351"
     CDS             complement(7406..9124)
                     /gene="proS"
                     /locus_tag="KW011_003351"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBH7661613.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="KW011_003352"
     CDS             complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="KW011_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HBH7661614.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="KW011_003353"
     CDS             complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="KW011_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HBH7661615.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="KW011_003354"
     CDS             complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="KW011_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HBH7661616.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11316..11969)
                     /gene="metI"
                     /locus_tag="KW011_003355"
     CDS             complement(11316..11969)
                     /gene="metI"
                     /locus_tag="KW011_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HBH7661617.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11962..12993)
                     /gene="metN"
                     /locus_tag="KW011_003356"
     CDS             complement(11962..12993)
                     /gene="metN"
                     /locus_tag="KW011_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HBH7661618.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13181..13756
                     /gene="gmhB"
                     /locus_tag="KW011_003357"
     CDS             13181..13756
                     /gene="gmhB"
                     /locus_tag="KW011_003357"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HBH7661619.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3401 a         3545 c         3717 g         3338 t
ORIGIN      
        1 cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt
       61 tccaggagga acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag
      121 atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc
      181 cacaaaacag cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgtggcgtga
      241 tttttgactg ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat
      301 atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata
      361 tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc
      421 gtatgcgtca gtacaccaac gaatatcttg ataacattac gccgccgttc acgaaagcct
      481 tgtttaccta cgtcaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca
      541 ccgcatatca aaaaagcccg gttggctgcc tgttttatga ttttttcggc gggaataccc
      601 tcaaggctga tgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc
      661 cacacctgga agcggaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg
      721 ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca
      781 gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg
      841 atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc
      901 cgcgccgtga atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac
      961 aatggccggt tcatgcggtg attaccaact ccacctatga tggcttgctc tacaacaccg
     1021 actggatcaa acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt
     1081 acacccattt tcatccgatc taccagggga aaagtggtat gagcggcgag cgtgttgcgg
     1141 gaaaagtgat cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt
     1201 cgctgatcca cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc
     1261 ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagacggcg gcggcgatgc
     1321 tgcgtggtaa tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc
     1381 gcaaagaggt ccagcggttg cgggaagagt ctgacggttg gtttttcgat atctggcaac
     1441 cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc aggcgaacag tggcacggct
     1501 ttaacgatgc ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac
     1561 cggggatgga cgaacagggc aatatgagcg aggaggggat cccggcggcg ctggtggcaa
     1621 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
     1681 tctttagtat tggtatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
     1741 ttaaacgctc ttacgatctc aacctgcgga tcaaaaatat gctacccgat ctctatgctg
     1801 aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
     1861 tgattcgtaa acacgatctt cccggtttga tgttgcgggc attcgatact ttgccggaaa
     1921 tgatcatgac gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg
     1981 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg
     2041 taccgctgtt gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc
     2101 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
     2161 cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg
     2221 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg
     2281 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagcttt
     2341 ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
     2401 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt
     2461 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
     2521 tgatgatatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
     2581 ccgtgtcgat ccatacacgc aaaaacgctt cacgttcttt aacgatccgg acgggctgcc
     2641 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg
     2701 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct
     2761 cacgccagat tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt
     2821 tggtgcagtg gcggacggaa aatccgggcg tcactctacg cgctatccat gtgcatcatg
     2881 gtttaagtgc taatgccgat gcctgggtta ggcattgcga gaacatctgc caacagtggc
     2941 aggtgccgct ggtggtcgag cgcgtgcaac ttgcgcaaga aggactgggc attgaggccc
     3001 aggcgcggca ggcgcgttat caggcatttg ctcgtaccct gttacccggt gaagtgctgg
     3061 tcaccgcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta
     3121 gcggccctgc cgggctttcg gctatggggg aagtttcgga atttgccgga acacggctta
     3181 tccgcccgtt gcttgcccgc acgcgggggg aactggagca gtgggcattg gcgcatggtt
     3241 tacgctggat tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc
     3301 tgcgtgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca acggcccgca
     3361 gcgccgcact ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag
     3421 cacactgcca aacgtcgcag gggacgctgc agattgcgcc aatgctggcg atgagtgatg
     3481 cccgtcgcgc ggcgattatc cgccgctggc tggcagggca gaatgcaccg atgccttccc
     3541 gcgacgcgct ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
     3601 gtttacgttt tggcgcgttt gaaatacggc gctatcagtc gcaactgtgg tggattaaat
     3661 ccgtcactgg gcaaagcgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat
     3721 taccggcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc cctccgcgtg
     3781 cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt gtcgggcgta
     3841 acggcggacg taagctaaag aaaatctggc aagagctggg cgtgccgccg tggctacgtg
     3901 acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga
     3961 cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta
     4021 gttaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat
     4081 tcgcttacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgc
     4141 agctcgcggg tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact
     4201 aaatcactgg cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa
     4261 tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgca
     4321 tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct
     4381 gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc
     4441 cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat
     4501 taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca
     4561 agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg
     4621 caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt
     4681 tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac
     4741 cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag
     4801 cgtatgatgc tgcgcctgct ggcgtggctg aaatatgctg atgaacgtct gcaatttacc
     4861 cgtggtttgt gtgccgatta tgagccggaa gcgtggctgc gtaacgatca cctgggcatt
     4921 gatttgtgga ttgagctggg gctgccggat gagcggcgga ttaagaaagc ctgcacccag
     4981 gccgcaaaag tggcgctgtt tacctataat agccgggcgg cgcaaatctg gtggcagcaa
     5041 aatcagagca aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa
     5101 ctggcgaaag taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat
     5161 ggcgtgatct ggttatcgga tgataagaat aatctggaag tgaacttaac cgcctggcaa
     5221 caaccttcat gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca
     5281 ccgccattcg tgcgcagggc gcgggcgggc agcatgttaa taagacctca acggctattc
     5341 atctgcgttt tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctgctcg
     5401 ccgccagcca tcatttgatc agcatgatgg cgtaattgtc attaaggcac aggaataccg
     5461 cagtcaggaa ctgaaccgcg aagcggcgct ggcccggctg gtggcagtga ttaaagattt
     5521 aacaacagaa caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag
     5581 gctggcatag aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg
     5641 tcgggaataa aaagaaggaa tggatggtga aaaaagcgat agtgacagcg atggctgtaa
     5701 tcagcctctt tactctgatg ggatgtaata atcgggccga agtcgatacg ctttctccgg
     5761 cgcaggctgc cgaactaaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgccg
     5821 attgcgaagg gatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg
     5881 agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc
     5941 gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga
     6001 aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc aattgaatcg cagttcaact
     6061 atacgctgga accggcacaa tccagtttac ctatgacgcc gatgaccctg cgaggcatgt
     6121 atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg
     6181 tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac
     6241 cggtgttact gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc
     6301 caaccaaagt attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta
     6361 gtttggggca gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg atgcgctgcg
     6421 cttatcaggc ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga
     6481 taaggcgttc acgacgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag
     6541 cccaacaaag cgacccgcct tgatataaat ccctggcttc aggtgcccaa catactgtaa
     6601 ctgcgtttct ggcttaaacg cagagacatc gccagtacgc atatgcatca gatcggcagg
     6661 gctaatccag cctgattgcg ccagcgtaag cggatgcccg gctttggcaa atgcatcagt
     6721 gacaaattcc gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc
     6781 tttcgccagg ccgctgacgc actgttggcg aaaatcctcg gagaacggat tcagtgagca
     6841 catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataactgct
     6901 atctttgatt ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg
     6961 gactcgtaag acgaaaagct tatcactatg cttcatcgct tttttaaggg aaacgatctg
     7021 gacgccagcg cctgtcgctt ctgcaacgtt attatcaccc agatagattg caacgtgact
     7081 cacagaggaa gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa
     7141 cagcaaatcg ccgggtttga gatcaggtac cgttatttct tttattgatt gttcggtgaa
     7201 agagctttga tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc
     7261 tgatgggtct ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag
     7321 aagcaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta aaaaagggcc
     7381 ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga
     7441 tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt
     7501 cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat
     7561 cagcaaacat cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg
     7621 cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt
     7681 tcatcggcag aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgccgcgtt
     7741 cgtcgtagtt ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac
     7801 ccatcgtcag gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg
     7861 agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg
     7921 taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg
     7981 gggtagcgac atcgcgatcc cagttgatac cgaagtagtg tttaccatcg atgttagcac
     8041 cagcagcgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt
     8101 ttaccggacc cagtgaaccc ggaccggctt taaccacagc acgaatttct tcttcggtcg
     8161 cgaaagtcag cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt
     8221 gatcaccgcg caccagcagc gcaaccagcg ggaagctgct gccttcaacc gctttaacca
     8281 gcagagtctt aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg
     8341 ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt
     8401 tcggcgcgat agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga
     8461 ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga
     8521 tagaaccggt gtcggcttgc acggcgcgga aatccagccc catgcggctg aagattttgc
     8581 tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag
     8641 agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt
     8701 cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa
     8761 gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac
     8821 gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct
     8881 cttgccacaa atcggctggc tgaaccaccg gcatcgacac ctcgattgca ccggcgttgt
     8941 tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc
     9001 aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc
     9061 tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac
     9121 gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa
     9181 aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg
     9241 cggttccagc gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc
     9301 atgtagccag acggcatagg ttttgcccgt ttcctcacct ttacgatagg ccgggcgcgg
     9361 gtcctgcgcc agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt
     9421 caaaagctgc ttttcgacct ctgcggtaaa actcaccgcc atctctgcag ctggcgcact
     9481 ttgcgcatag ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat
     9541 atccactacc ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgtc
     9601 tttatggcaa acaacctctt tcagctctac cagcgacata ccaattgggt tagggcggaa
     9661 agtagagcgt gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac
     9721 agtcggacgc cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc ataaatggct
     9781 gaacgcttcc aggccgcgaa cggcgtcggc ctggttgtag ggagcaatga gatgcagttc
     9841 gccgttggcg ctttttacca gaccaggctg gcgcggaacg gcgaattttt ctttataggg
     9901 cgagcgaata acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc
     9961 gcagagccga tacatacagc ctgacgatag cagcctagcg taccgctggt gacttcgcag
    10021 ctatgcagta ataccgcatt ggctttcatt ttagaggcgt tgatttgcat ccgcttacgc
    10081 gcggttggaa tgctcggcgg agagtcctga ttagaggcct ggcaagagtc gccactgact
    10141 tcaccgagat cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc
    10201 ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt
    10261 tgaacgggtt cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact
    10321 aaacagatcg gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg
    10381 aatcaggagc ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa
    10441 ttgtggaaac agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt
    10501 gctgcttcgt aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg
    10561 tctttgttat cttcacgcgt tacgatcagg ttcacgtacg gggagtcttt atcttcgaca
    10621 aagataccgt ctttcgctgg agtcaggcca atctggctgg cataggtggt attgataact
    10681 gccagagcga tttgcgcgtc gtccagagat cgaggcagct gcggtgcttc cagctcaaca
    10741 attttcagat tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag gccaacgcca
    10801 tctttcagtt tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt
    10861 ggatcgtttg gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc
    10921 ttggagtaac cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca
    10981 cgatctttca gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg
    11041 cctttgctca atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc
    11101 aggccatatt tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca
    11161 acaatcacgc cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt
    11221 gccagtgatc caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct
    11281 tattccttct tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg
    11341 atgcggtcgc ctgcgaactg aattaaataa accagaatga ccagcaatac cagtaccgta
    11401 ttcatcaccg tcgcgttgta gccgatgtag ccatactgat agccaatctg acctaaacca
    11461 ccggcaccga ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt
    11521 gccgcattca ccagacccgg caacgcttcc ggtaacagca ctttacggac gatctgcatt
    11581 ggcgtggcac ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg
    11641 ttctcgacca tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aattgctgcc
    11701 tgcaaaccaa tcgatgtacc gacaataacg cgggtaaacg gaatcatcca taccagcaag
    11761 ataatgaacg ggatggaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc
    11821 gcgttagcaa taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca
    11881 atcacaaagc caaaaaatcc ggatacgaag gtcattgcca gcgtttccca tacgccacga
    11941 accagcagcc acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc
    12001 ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat
    12061 gatgccgaat ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac
    12121 gttgaaacga cgcgcggttt cagaaagcag tggggcatcg accgattgac cggtaaactc
    12181 cagacgcagc atcggcacgc agtcagtaaa tggctccgct tgcagacgtt cctggtaatc
    12241 ttccgggata tccagatgca gggtcgactg aataaacttc tgcgccagcg gcgttttcgg
    12301 atgcgagaac acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc
    12361 cacgcaatca caaatacgct tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa
    12421 acccagacgg cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc
    12481 cagcgcgctg gtggcttcat cacacagcaa tactttggga ttgctggcta atgcacgggc
    12541 aatcgccaca cgttgtttct gcccaccgga aagattcgac gggtagctat catgcttatc
    12601 gccaagacca accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt
    12661 gttgtccagc tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt
    12721 aaaatgctgg aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag
    12781 cgtggtcaat tcttggccat cgaccagcac gctaccctcg gttgggcgct ccagcaggtt
    12841 tacacaacgt ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg
    12901 tccagctggc acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccctggtg
    12961 gaacactttg gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta
    13021 gtcgtggcat ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca
    13081 tccagacgtc taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg
    13141 cgatactagc gtcccatgcc ttattaagga gctataaaag gtggcgaaga gcgtacccgc
    13201 aatttttctt gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga
    13261 caactttgaa tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt
    13321 tgcgctggtg gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca
    13381 gtttgaaacg ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga
    13441 tggtatctat tattgcccgc atcatccgca gggtagtgtt gaagagtttc gccaggtctg
    13501 cgattgccgc aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga
    13561 tatggccgct tcttatatgg tgggcgataa attagaagat atgcaggcag cggttgcggc
    13621 gaacgtggga acaaaagtgc tggtgcgtac gggtaaacct attacacctg aagcagaaaa
    13681 cgcggcagat tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca
    13741 aaaaccggcg caatgattaa aagatgagcg gttgaaataa aaatgcattt ttccgcttgt
    13801 cttcctgagc cgactcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca
    13861 cacaaacagc cggttcggtt gaagagaaaa aatcctgaaa ttcagggttg actctgaaag
    13921 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagctgcgtc gcaactgctc
    13981 tttaacaatt tatcagacaa t
//
LOCUS       DAESHI010000112        13905 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.113, whole genome shotgun sequence.
ACCESSION   DAESHI010000112 DAESHI010000000
VERSION     DAESHI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13905
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.113"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            244..1497
                     /gene="kch"
                     /locus_tag="KW011_003358"
     CDS             244..1497
                     /gene="kch"
                     /locus_tag="KW011_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HBH7661620.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(1552..1725)
                     /locus_tag="KW011_003359"
     CDS             complement(1552..1725)
                     /locus_tag="KW011_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HBH7661621.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1868..3328
                     /gene="cls"
                     /locus_tag="KW011_003360"
     CDS             1868..3328
                     /gene="cls"
                     /locus_tag="KW011_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBH7661622.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3363..3692
                     /locus_tag="KW011_003361"
     CDS             3363..3692
                     /locus_tag="KW011_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HBH7661623.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3745..4749)
                     /gene="oppF"
                     /locus_tag="KW011_003362"
     CDS             complement(3745..4749)
                     /gene="oppF"
                     /locus_tag="KW011_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HBH7661624.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4746..5759)
                     /gene="oppD"
                     /locus_tag="KW011_003363"
     CDS             complement(4746..5759)
                     /gene="oppD"
                     /locus_tag="KW011_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HBH7661625.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5771..6679)
                     /gene="oppC"
                     /locus_tag="KW011_003364"
     CDS             complement(5771..6679)
                     /gene="oppC"
                     /locus_tag="KW011_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HBH7661626.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6694..7614)
                     /gene="oppB"
                     /locus_tag="KW011_003365"
     CDS             complement(6694..7614)
                     /gene="oppB"
                     /locus_tag="KW011_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HBH7661627.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7700..9331)
                     /gene="oppA"
                     /locus_tag="KW011_003366"
     CDS             complement(7700..9331)
                     /gene="oppA"
                     /locus_tag="KW011_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HBH7661628.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10069..10716)
                     /gene="ychE"
                     /locus_tag="KW011_003367"
     CDS             complement(10069..10716)
                     /gene="ychE"
                     /locus_tag="KW011_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HBH7661629.1"
                     /translation="MIQTFLDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11194..13869
                     /gene="adhE"
                     /locus_tag="KW011_003368"
                     /gene_synonym="adhC"
     CDS             11194..13869
                     /gene="adhE"
                     /locus_tag="KW011_003368"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBH7661630.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3655 a         3438 c         3232 g         3580 t
ORIGIN      
        1 tccttatagc cggtcagtgc attcactacc acaagcagat aataacttta tctgtatttc
       61 tttatatgat gtgagcatat tcaggaaaaa tgacctttcc tgaggtacag ttgctcgctc
      121 aaatcctttg attactgcgg ataaagtcat ctactattgt ttaacacatt cacgcgctgc
      181 atgtatgttc agaaaatgtg cacgctaaga taattaaaca aaattgtggt tacagaggta
      241 tcagtgagtc actgggctac attcaaacaa acagcaacaa atttatgggt gacccttcgg
      301 cacgacattc tcgcgctggc cgtcttttta aatggattgc ttatttttaa aacaatctat
      361 ggtatgtcgg tcaatttgct tgatattttc catatcaaag cattttcaga gttggatctc
      421 tccttgctgg caaacgcccc actatttatg ctcggcgtct ttcttgtcct gaactccatt
      481 ggcttactgt tccgggcaaa gctcgcatgg gcaatcagta tcattttgtt gttgatagcg
      541 ctaatttaca ccctgcattt ttatccctgg ctgaaattta gtattggatt ttgcattttt
      601 acgctggtgt ttttgctgat actgcgcaaa gacttctccc acagtagcgc cgcagccggg
      661 acaatttttg catttattag tttcacgaca ttactgtttt actcaaccta cggtgcgctt
      721 tatttaagcg aaggttttaa tccgcgaata gaaagtttga tgaccgcgtt ctatttttcg
      781 atagaaacca tgtcaaccgt cggctatggc gatattgtcc ctgtttctga atcagcacga
      841 ttgttcacta tttcggtcat tatttccggc attaccgttt ttgccacatc tatgacctca
      901 atttttggtc cgcttatccg cgggggattc aacaaacttg taaaaggaaa caatcataca
      961 atgcatcgta aagatcattt tattgtttgc ggacattcga ttctcgccat caatacgatc
     1021 ctgcaactga atcaacgcgg acaaaacgta acggttatca gcaacttgcc tgaagatgat
     1081 atcaagcaac ttgagcaacg cttaggcgat aacgctgatg ttatccccgg tgacagtaat
     1141 gacagttcag tattaaagaa agcgggaatc gatcgttgcc gggccattct ggcgctgagt
     1201 gataacgatg cagataacgc gtttgttgta ctctcggcaa aagatatgag cagtgatgtc
     1261 aaaacagttc tcgccgtcag tgatagcaaa aacctgaata agattaagat ggtacatccg
     1321 gatatcattc tctcaccgca actgtttggc agcgaaattc tggcgcgagt tttaaatggt
     1381 gaagagatta ataatgatat gctcgtttca atgttgttga actccggtca tggcattttc
     1441 agcgataacg atgaacaaga aacgaaagct gacagtaaag aatcagtgca aaaatagagt
     1501 ttaaagacga aagagtaaac ttattgggca ccgcaatggt gcccagtata tttagatatt
     1561 gtagatcgca aacaataacg agttacgctg tttgtttaga atgcacttcc tgatgctgtg
     1621 gatacgcata tttcggcgcg attgcccctc aagccaacgc gatttacgtc ggctagcctg
     1681 acgctgcatg cgccagcgcc ccacttccgt tctactccgc ttcatgttaa ctactctatg
     1741 caataacaga atggtcatta tacccccacc cccgacgctg accagtgctt ttcccgcgtt
     1801 tttatttgcc agatgaatcc atatgcgtaa actcataaca atgcgctttc aaaaggattt
     1861 ctaatctatg acaaccgttt atacgttggt gagttggttg gccattctgg gatactggtt
     1921 gctcattgca ggcgtaactt tacgcattct aatgaaacga cgcgcagttc cctccgcgat
     1981 ggcctggctg ttgattattt acattctgcc gttagtcgga attattgcct atcttgccgt
     2041 tggcgagctc catttaggca aacgccgcgc tgagcgcgcc agagcgatgt ggccttccac
     2101 cgcaaaatgg cttaacgacc ttaaagcctg taagcatatc ttcgccgaag aaaatagcag
     2161 tgtcgctgcg ccattattca agctttgcga gcgtcgtcag gggatcgctg gggtcaaagg
     2221 gaatcagcta caactgatga ccgagtcaga tgatgtgatg caggcgttaa tccgcgacat
     2281 ccagctcgcg cgccataata ttgagatggt gttttatatc tggcagcccg gcggcatggc
     2341 ggaccaggtg gctgaatcat taatggcggc tgcacggcgt ggtattcatt gccgattgat
     2401 gctcgactcc gccgggagtg tggctttttt ccgcagcccg tggcccgagc taatgcgtaa
     2461 tgccggtatt gaagtggtcg aagccttaaa ggtcaatctg atgcgtgtgt ttttacgccg
     2521 tatggacctg cgccaacatc gcaagatgat catgatcgat aattacatcg cgtacaccgg
     2581 cagcatgaat atggtcgatc ctcgctactt caaacaagat gcgggcgtag ggcaatggat
     2641 tgatctgatg gcgcgtatgg aaggccccat cgccaccgcg atggggatta tttattcctg
     2701 cgactgggag attgaaaccg gaaaacgtat tctgccgcca ccaccagatg tcaatattat
     2761 gccgtttgaa caggccagcg gtcacaccat tcacacaatt gcttctggcc ccggctttcc
     2821 ggaagatctc attcaccagg cattattgac tgcggcttat tcggcgcgtg aatatttgat
     2881 catgacaacg ccctactttg tgccaagcga tgatttactt catgcgattt gcacggcggc
     2941 gcagcgcggg gtggatgtca gtattatcct tccgcgaaaa aatgactcga tgctggtcgg
     3001 ctgggccagt cgcgcattct ttacggaact gcttgctgct ggggttaaaa tttatcagtt
     3061 tgaaggcggg ttactgcata ccaagagcgt gctggtcgat ggcgaactaa gtctggttgg
     3121 caccgttaac cttgatatgc gtagtctgtg gctgaatttc gagattaccc tggcaatcga
     3181 cgataaaggt tttggtgcag accttgccgc cgttcaggac gattatattt cgcgttcacg
     3241 tctgctcgat gcccgtttat ggctaaaacg tccattatgg caacgtgtcg ccgagcgact
     3301 gttttacttc ttcagcccgt tgctgtaaaa cgtcgccaac agaggttaaa caggtagtga
     3361 ctatggatat ggatctaaac aatcgcctga ctgaagatga aacgcttgag caggcctacg
     3421 atatttttct cgaactggct gccgacaatc tcgacccagc tgatgttctg ctgttcaatc
     3481 ttcagtttga agagcgcggc ggcgcggagt tattcgatcc ggcggaagac tggcaggaac
     3541 atgttgattt cgatttgaac ccggactttt ttgccgaagt ggtgattggt ctggcggaca
     3601 gtgaggatgg tgaaatcaac gatgttttcg cccgcattct gttatgccgc gaaaaagatc
     3661 acaaactttg ccatatcatc tggcgggaat aaataatcgc tttcggcgtg aaaaagggct
     3721 gacaactgtc agcccttatt gtgtttaaag cggatcgact ttcaggcaag aaacggcgtg
     3781 tctgaaactc ccttccagca caggacgtgt tttggcgcac tccggaccgg caatcgggca
     3841 acaggtacgg aaaacacaac cggaaggcgg gttgatcggc gacggtaatt ccccttccag
     3901 taactggatg gttttgttct tctccagatc cggatcaggt atggggactg ccgacatcaa
     3961 tgccctggtg taaggatgta gtggattgtg gtagacctca tcataggtcc ccagttctac
     4021 cgcatggccg agatacatca ccaacacacg atcggaaatg tgttttacca cggccaggtc
     4081 atgagcgata aaaattaatg acaatcccat ctcacgttgc agctgctgga gcaggttgac
     4141 cacctgcgcc tgaattgaca cgtccagcgc cgaaatcggc tcatcgcaga taatcagctt
     4201 cggttcaaga ataagagcac gagcaatccc gatacgctgg cactgcccac cggagaactc
     4261 atgcggatag cggttaatca ggttaggcaa taacccgact ttcagcatca tcgccttcac
     4321 gcgctcgcga acttcctggc gtgacatttt cggatgataa gtacgcagtg gttcagcgat
     4381 gatctcgccg atggtcatac gcgggtttag cgatgccaac ggatcctgga aaatcatctg
     4441 aatatcactg cgaacggcac gccattcatc gggcttcatg cccagcaact ctttacctaa
     4501 ccaggcaaca tgaccgtcgg tcgctttaac caggccaatg atggcgcgag caaaggtgga
     4561 cttaccgcat cccgattccc ctaccacacc taatgtttcc ccttcataca ggcgaagcgt
     4621 gacaccatcg acggctttga gagttttcgg cggttgccag aaccactgtt tgccatcttt
     4681 gatttcaaag tgcactttca gatcggcgat ttcgaggagg acttttcttc cttcggttac
     4741 agcattcata acagttcctc caccggttta aagcaagcac gcagacggcc aggcgtaaac
     4801 tcttccagcg gcggtgcgct actacaaatt tccatcgcat gcggacaacg tggctggaac
     4861 gggcaacctt tcggtaatcg cagcaggttt ggcggattac cagggatggt caacattgtt
     4921 tcaccttccg catcgagacg cggcaccgcg ttgagcaaac cgatagaata aggatgaacg
     4981 ggttgataaa agacatcgcg cgcgttgcca tattccatcg tgcgcccggc gtacattacc
     5041 agcactttgt cgcagatccc cgccaccacg ccaagatcgt gggtaatcat aatgatggcg
     5101 gtattaaatt cccgcttcag ttcatttaat agcgtcatga tctgcgcctg tacggtgacg
     5161 tccagcgcag tagtgggttc atccgcaatc agcagcttag gtcgacatag caatgccatc
     5221 gcaatcataa ctcgctgacg catgccacca gaaaattcgt gcgggtacat tttcatgcgt
     5281 ttacgtgctt ccggcatttt taccgcatcg agcatccgca ccgactcttc aaacgcctca
     5341 gctttgctca tgttcttatg cagcatcagc acttccatca actgctcacc gacgcgcata
     5401 tagggattca acgaagtcat tgggtcctga aaaatcattg agatttgttc agcgcgcagt
     5461 ttattgagtt cacgctctgg caaattgagg atttcacgcc cattgaaggt tgccgatccg
     5521 ccaatacgtc cgttggcagc caacaggccc atcaacgcaa atgcagtttg cgatttaccc
     5581 gaaccagact cacctacaat gcccagcgtt tctccggcac gtagagaaaa attcaaatca
     5641 ttgaccgccg tgacatcgcc gtccggggta ctaaaggtga cacgcaaatc tttcacgttc
     5701 agcagtgcgt cagcctgttg ttgtgcgagc ggcacagttg cagtttcaat tacgctcatg
     5761 gctgcactcc ttaacgatct ttcgggtcga gagcatcacg caagccatcg ccgataaagt
     5821 tgaaacaaaa cagcgtcacc acgaggaatc ccgctgggaa caacaataac catggagaga
     5881 cttccatcga gttcgcgcca tcactcagca atgcacccca gctgcttaac ggctcttgcg
     5941 tacccaaccc caggaagcta aggaaagatt caaagaggat catgctgggc accagaagtg
     6001 atgcgtagac caccaccaca ccgagtacat tcggcacaat gtggcgaata acaatgcccg
     6061 atgtcgatac accgccaact tgtgccgcct caataaactc tttgcgcttc agactcaggg
     6121 tttgtccacg cacaatacga gccatatcca gccaggaaac catgccaatc gccacgaaaa
     6181 tcagcaggat gttttgaccg aaaaaggtca caagcaaaat gacgaagaac atgaatggga
     6241 aggagttgag gatttccagc agacgcatca ttaccgaatc cactttaccg cccagataac
     6301 cggaaagcga accgtaaagt gtccccacga ccactgccac cagtgccgca gcaacaccga
     6361 ccatgagtga gatacgcccg ccaatcgcaa cgcgcacaag caggtcgcga ccggatgagt
     6421 cagtaccaaa gtagtgaccg gactccatat ccggggcgct ggacatcatc gcccagtcag
     6481 tatcgtcata ggcaaactgc gaaagcatcg gtgccaggat tacaaataac gcgatcagca
     6541 ccagcactat cagactggca accgccgcac ggttatgcat aaaacgtcga cgtgcgtcct
     6601 gccacaagct gcgcccttcg acctccagct tttcactgaa attttccagc gtctcgctgt
     6661 ttttcttact taacatcatc gcgagctcca gtatcagtaa cggattttcg ggtcgataac
     6721 cgcatatagc acatcgacaa tggcattaaa caaaatggtt aaagcaccaa ccaggatggt
     6781 caggcttaac actaaggaat agtcacggtt caatgcgcca ttaacgaaca gttgtccaat
     6841 ccccggcaaa ccataaatgg tttcgataac catagaaccg gtaataatgc cgacaaatgc
     6901 ggggcccata taagagagca cgggtaacag agcaggtttt aatgcgtggc gtaaaatgat
     6961 ccgccgcata ggtaaccctt tcgcccgggc agtacgaata aagttggagt gtaatacttc
     7021 aatcatagag cctcgggtaa tacgcgcaat actggcgata taagcgagtg acaacgccac
     7081 catcggcaat atcatgaatt taagcgcccc accattccag ccaccgcccg gcagccaatg
     7141 caaaatgatc gcaaatatca tgactaataa tggcgcaacc acaaaactgg ggataacaac
     7201 cccggtcatt gccagcccca tcacggtata gtcccatttg gtgttttgtt ttaatgcgac
     7261 aataacgcca gcactaacac ccagtattac cgcaaggaaa aatgcggcgg ctcccagttt
     7321 ggcagaaacg ggaaaactgg atgcaaccag gtcattgacc gaataatctt tatatttaaa
     7381 cgatggaccg aaatcaccgt gcgccagttg tttcaggtag ctgaaatact gtgtcatgat
     7441 cggatcatta agatgatatt tcgcttcgat attggccatc acttccggcg gtaaagtacg
     7501 ttcgccggta aaagggcttc ccggcgcgag gcgcatcata aagaacgaaa tagtaataag
     7561 aataaatagc gtcggaatcg cttccagaca gcgacgtaga ataaatttta acattgccct
     7621 accttctggc ctgtgccttc tgtaatgcga taaaaatcag acaccgtgga gcaggacact
     7681 cctgccccac gtattgccat tagtgcttca caatgtacat attccgggtg taggtattat
     7741 ccagcggatc tttgccggta tagccaccaa cccacggttt caccagacgc gcattcacgt
     7801 agtaataaac aggaacaatg gccgaatcct tatccagctg ttgttctgct ttagtgtaca
     7861 gagctgtgcg ctgcgcctcg tcagtcactt tcagcgtttc cgccataatg ctgtcaaagg
     7921 ccgggctctt ataatgcgcg gtattcatcg agctgttcga cagcatggtg ttcaggaagg
     7981 aagttggttc gttgtagtct gcacaccagc ctgcacgggc cacatcaaaa gtgccctggt
     8041 gacgggtgtc gaggaacgtt ttccactcct ggttaaccag tttgacgttt acaccaatgt
     8101 ttttcttcca caatgaagag gcagcaatcg ccagcttttt atgcagatca gaggtgttat
     8161 acagcaggtt gatggtcaac ggtttgtctg cggtataacc cgcttcagcc agcagttttt
     8221 tcgcttcttc gttacgtttt tcctggctcc agccaaacca ttctggctga gtcaattttg
     8281 cgccatcagt atacggtgga gtgtaaccat aggcgggcat gtcgccctgc gctttcactt
     8341 tattaacaat gatatcgcgg tccataccta gtttcagtgc ggtacgcaca cgcacatcgt
     8401 tgaatggcgg tttctggttg ttaatttcgt aatagtaagt gcacaggtat ggatcaacgt
     8461 gaacttcgtc cgggatctct tttttcagct tctggaacaa ttcgatcggc atgttgttat
     8521 acgtcatgtc gatttcacca ctacggtagc ggttgacatc ggtaacttca gaagcaatag
     8581 gcaaataggt tacctggtta ataacggttt tcgcgttgtt ccagtaggtc gggctgcgtt
     8641 caagaacgat tcgttcgttt acgacccaat cttttaaggt ataggcaccg ttggtgacga
     8701 tattaccagg ctgggtccat ttttcgccga atttctcgat agcggctttt ggcaccggtg
     8761 aagttgatgg gtgaacaagt aatttataga agtacggaac gggttcactt aaggtgactt
     8821 ctaatgtgtg atcatcaata gctttcacgc cgagatcggt aatcggtttt ttcccttcaa
     8881 gaatttcatc aataccggcg atatgcccat attgcagata actggcatac ggagaagcag
     8941 tgttcggatc aacagaacgt tgccagctat acacaaagtc ttgtgctgtg actggcgtgc
     9001 tatcagacca tttcgcatct ttacgcaaat ggaaggtcca gactttcgcg tctttattat
     9061 cccaggattc agcgacgcca ggtgctggat gaccgtcaag atcgctgacc agtaagcctt
     9121 caaacaggtc tcggctgata ttagactccg gaacaccttc aattttgtgc ggatctaatg
     9181 actgaacttc tgaaccattg ttacgtacca gtgtttgttt ttccgccagt gtgacgcctg
     9241 cgggtacatc agctgccagc gcgacattcc ctgccattag cgcagccaga acgccagctg
     9301 ctactaaact tctcttggtg atgttggtca ttgttttttg gactccctca ttataattac
     9361 tggtattaac cagcatgtgt aatcccggat tgggaccctg cacaggctcg gagactttct
     9421 gctgtcaggt tactttatcg ttacgtatta ctatcaccga ctttatttat tctggtgatt
     9481 cagacgatca ccttatgtcg attcgtactg tgtctcccct gtcgagacac attattcttt
     9541 cgcgacgctc tttaattgtt aattattctc attaccaata aagaatcgtc tggcggttgc
     9601 ccggaaagta ccaaatgcgg ttcttactcg ccaatacatt ttgcaaaatt gttaagcaat
     9661 tctcttttat tgaccgtatc agaatctata gcttgcatcc atgccttaac aaacatcttt
     9721 caattcaatc aaatagagat tggctaagca tgtctatctt ttctgattac agttccagta
     9781 gtgaaatgca caataattta acaattgatt attatttagc actttcctct accaaaggca
     9841 gtggaattac taatatcatt tcaattattc ttcagcaagc ccaggattat gatgtggcga
     9901 aaataacatg atggtgagga gaagcagtta aggaaatcct tataactaac tgtaatttga
     9961 gaagaatatt tttacctatt ccttttgctt ttgataactg cgcgggaaca aagtaaaaat
    10021 aatgacgaag taaacggagc atggcagctc cgtttcattt gaaaggaatt aattaagcag
    10081 gccggggaaa atccccttaa taccagtaac gataaattca atccccaatg ccatcagcaa
    10141 tagccccata atacgcgtaa tcacgttgat gccggtctgg cgtaaaaccc gtaccagcca
    10201 cggtgccatg cggaacaatc cccagcaaca taaagcgaac aatgcaatag ccacaaagaa
    10261 accaaacaga tagctaatgc tgtgataacg cgtaccccag acgatggtag aactgatcgc
    10321 ccctggcccc gccatcaacg gcaacgccaa tggcaccaca ccaatgcttt cacgtaccgc
    10381 ggtttctgat ttttcttgct tgttctgttt atcctcgcca agcttgccgc tgatcatcga
    10441 catcgctatc gtcaccacca ggatgccccc tgcgatacgg aacgaatcaa ttgatatacc
    10501 aaaaagttgc agaatcgtgt cgccgagaaa aagcgagatc cacaagataa tggccacaga
    10561 caggttggct gtaaggttag ttttgtttcg cgctgccgct gtctgataac tggtcatgct
    10621 gataaagacg ggaataatcc ctaccgggtt gaccagcgca aataacccga tgaaaaattt
    10681 gaagtaaacg ggaaaatcca aaaaggtctg aatcacggtt agctccgaag caaaagccgg
    10741 ataatgttag ccataaatta aggttgaaaa gacgcgctga caatacgcct tttgacagca
    10801 tttttcacct cctaactact taaaattgct atcattcgtt attgttatct agttgtgcaa
    10861 aacatgctaa tgtagccacc aaatcatact acaatttatt aactgttagc tataatggcg
    10921 aaaagcgatg ctgaaaggtg tcagcttagc aaaaatttga tttggatcac gtaatcagta
    10981 cccagaagtg agtaatcttg cttacgccac ctagaagtga cgcattagag ataataactc
    11041 taatgtttaa actcttttag taaatcacag tgagtgtgag cgcgagtaag cttttgattt
    11101 tcataggtta agcaaatcat caccgcactg actatactct cgtattcgag cagatgattt
    11161 actaaaaaag tttaacatta tcaggagagc attatggctg ttactaatgt cgctgaactt
    11221 aacgcactcg tagagcgtgt aaaaaaagcc cagcgtgaat atgccagttt cactcaagag
    11281 caagtagaca aaatcttccg cgccgccgct ctggctgctg cagatgctcg aatcccactc
    11341 gcgaaaatgg ccgttgccga atccggcatg ggtatcgtcg aagataaagt gatcaaaaac
    11401 cactttgctt ctgaatatat ctacaacgcc tataaagatg aaaaaacctg tggtgttctg
    11461 tctgaagacg acacttttgg taccatcact atcgctgaac caatcggtat tatttgcggt
    11521 atcgttccga ccactaaccc gacttcaact gctatcttca aatcgctgat cagtctgaag
    11581 acccgtaacg ccattatctt ctccccgcac ccgcgtgcaa aagatgccac caacaaagcg
    11641 gctgatatcg ttctgcaggc tgctatcgct gccggtgctc cgaaagatct gatcggctgg
    11701 atcgatcaac cttctgttga actgtctaac gcactgatgc accacccaga catcaacctg
    11761 atcctcgcga ctggtggtcc gggcatggtt aaagccgcat acagctccgg taaaccagct
    11821 atcggtgtag gcgcgggcaa cactccagtt gttatcgatg aaactgctga tatcaaacgt
    11881 gcagttgcat ctgtactgat gtccaaaacc ttcgacaacg gcgtaatctg tgcttctgaa
    11941 cagtctgttg ttgttgttga ctctgtttat gacgctgtac gtgaacgttt tgcaacccac
    12001 ggcggctatc tgttgcaggg taaagagctg aaagctgttc aggatgttat cctgaaaaac
    12061 ggtgcgctga acgcggctat cgttggtcag ccagcctata aaattgctga actggcaggc
    12121 ttctctgtac cagaaaacac caagattctg atcggtgaag tgaccgttgt tgatgaaagc
    12181 gaaccgttcg cacatgaaaa actgtccccg actctggcaa tgtaccgcgc taaagatttc
    12241 gaagacgcgg tagaaaaagc agagaaactg gttgctatgg gcggtatcgg tcatacctct
    12301 tgcctgtaca ctgaccagga taaccaaccg gctcgcgttt cttacttcgg tcagaaaatg
    12361 aaaacggctc gtatcctgat taacacccca gcgtctcagg gtggtatcgg tgacctgtat
    12421 aacttcaaac tcgcaccttc cctgactctg ggttgtggtt cctggggggg taactccatc
    12481 tctgaaaacg ttggtccgaa acacctgatc aacaagaaaa ccgttgctaa gcgagctgaa
    12541 aacatgttgt ggcacaaact tccgaaatct atctacttcc gccgtggctc cctgccaatc
    12601 gcgctggatg aagtgattac tgatggccac aaacgtgcgc tcatcgtgac tgaccgcttc
    12661 ctgttcaaca atggttatgc tgatcagatc acttccgtac tgaaagcagc aggcgttgaa
    12721 actgaagtct tcttcgaagt agaagcggac ccgaccctga gcatcgttcg taaaggtgca
    12781 gaactggcaa actccttcaa accagacgtg attatcgcgc tgggtggtgg ttccccgatg
    12841 gacgccgcga agattatgtg ggttatgtac gaacatccgg aaactcactt cgaagagctg
    12901 gcgctgcgct ttatggatat ccgtaaacgt atctacaagt tcccgaaaat gggcgtgaaa
    12961 gcgaaaatga tcgctgtcac caccacttct ggtacaggtt ctgaagtcac tccgtttgcg
    13021 gttgtaactg acgacgctac tggtcagaaa tatccgctgg cagactatgc gctgactccg
    13081 gatatggcga ttgtcgacgc caacctggtt atggacatgc cgaagtccct gtgtgctttc
    13141 ggtggtctgg acgcagtaac tcacgccatg gaagcttatg tttctgtact ggcatctgag
    13201 ttctctgatg gtcaggctct gcaggcactg aaactgctga aagaatatct gccagcgtcc
    13261 taccacgaag ggtctaaaaa tccggtagcg cgtgaacgtg ttcacagtgc agcaactatc
    13321 gcgggtatcg catttgcgaa cgccttcctg ggtgtatgtc actcaatggc gcacaaactg
    13381 ggttcccagt tccatattcc gcacggtctg gcaaacgccc tgctgatttg taacgttatt
    13441 cgctacaatg cgaacgacaa cccgaccaag cagactgcat tcagccagta tgaccgtccg
    13501 caggctcgcc gtcgttatgc tgaaattgcc gaccacttgg gtctgagcgc accgggcgac
    13561 cgtactgctg ctaagatcga gaaactgctg gcatggctgg aaacgctgaa agctgaactg
    13621 ggtattccga aatctatccg tgaagctggc gttcaggaag cagacttcct ggcgaacgtg
    13681 gataaactgt ctgaagatgc attcgatgac cagtgcaccg gcgctaaccc gcgttacccg
    13741 ctgatctccg agctgaaaca gatcctgctg gatacctact acggtcgtga ttatgtagaa
    13801 ggtgaaactg cagcgaaaaa agaagccgct ccggctaaag ctgagaaaaa agcgaaaaaa
    13861 tccgcttaat cagtagcgct gtctggcaat ataaacggcc ccttc
//
LOCUS       DAESHI010000113        13853 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.114, whole genome shotgun sequence.
ACCESSION   DAESHI010000113 DAESHI010000000
VERSION     DAESHI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13853
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.114"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(1..736)
                     /gene="dsbG"
                     /locus_tag="KW011_003369"
                     /pseudo
     CDS             complement(1..736)
                     /gene="dsbG"
                     /locus_tag="KW011_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
     gene            1108..1671
                     /gene="ahpC"
                     /locus_tag="KW011_003370"
     CDS             1108..1671
                     /gene="ahpC"
                     /locus_tag="KW011_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HBH7661631.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1842..3407
                     /gene="ahpF"
                     /locus_tag="KW011_003371"
     CDS             1842..3407
                     /gene="ahpF"
                     /locus_tag="KW011_003371"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBH7661632.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3528..3956)
                     /gene="uspG"
                     /locus_tag="KW011_003372"
     CDS             complement(3528..3956)
                     /gene="uspG"
                     /locus_tag="KW011_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HBH7661633.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4177..5415
                     /locus_tag="KW011_003373"
     CDS             4177..5415
                     /locus_tag="KW011_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HBH7661634.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDILGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            complement(5646..6056)
                     /gene="rnk"
                     /locus_tag="KW011_003374"
     CDS             complement(5646..6056)
                     /gene="rnk"
                     /locus_tag="KW011_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HBH7661635.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6286..7092)
                     /gene="rna"
                     /locus_tag="KW011_003375"
     CDS             complement(6286..7092)
                     /gene="rna"
                     /locus_tag="KW011_003375"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HBH7661636.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            complement(7206..8669)
                     /gene="citT"
                     /locus_tag="KW011_003376"
     CDS             complement(7206..8669)
                     /gene="citT"
                     /locus_tag="KW011_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HBH7661637.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8720..9598)
                     /gene="citG"
                     /locus_tag="KW011_003377"
     CDS             complement(8720..9598)
                     /gene="citG"
                     /locus_tag="KW011_003377"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBH7661638.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9573..10124)
                     /gene="citX"
                     /locus_tag="KW011_003378"
     CDS             complement(9573..10124)
                     /gene="citX"
                     /locus_tag="KW011_003378"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBH7661639.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(10128..11660)
                     /gene="citF"
                     /locus_tag="KW011_003379"
                     /pseudo
     CDS             complement(10128..11660)
                     /gene="citF"
                     /locus_tag="KW011_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
     gene            complement(11671..12579)
                     /gene="citE"
                     /locus_tag="KW011_003380"
     CDS             complement(11671..12579)
                     /gene="citE"
                     /locus_tag="KW011_003380"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBH7661640.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12576..12872)
                     /gene="citD"
                     /locus_tag="KW011_003381"
     CDS             complement(12576..12872)
                     /gene="citD"
                     /locus_tag="KW011_003381"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBH7661641.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12887..>13853)
                     /gene="citC"
                     /locus_tag="KW011_003382"
     CDS             complement(12887..>13853)
                     /gene="citC"
                     /locus_tag="KW011_003382"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBH7661642.1"
                     /translation="VDTTVEVFITVTRDEKLITCGGIAGNIIKCVAISESVRGEGLAL
                     TLATELINLAYERHSTHLFIYTKTEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRY
                     AESLKKFRHPGNKIGCIVMNANPFTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYED
                     RLDLVLKGTADIPRLTVHRGSEYIISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLA
                     PALGVTHRFVGTEPFCRVTAQYNQDMRYWLETPTISAPPIELVEIERLRYQEMPISAS
                     RVRQLLAKNDLTAIAPLVPAVTLHYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3401 a         3507 c         3687 g         3258 t
ORIGIN      
        1 aatgatatta agcgttttct gatcgggcaa ccccacggcc tgttgcagcg tattttcctt
       61 actcatgtaa tagatagccg gcgtgacatt tgcccccaga tcgtccatca gtttctcatt
      121 gtcacttaac actttcattt gctctgtact tacgtttgca ggcacgttta gcttaagctt
      181 gccaccagag gcttcatatt gttgccaggt ttttgcggga tctttggagg caagaattgc
      241 cgctgctgtc gccgggcttt ctggcttgat aaccccaacc aacaatgttc ttaattgcac
      301 tttgccagaa tctacccacg ggcgcgcctg ctgccagaac tgtttacaat atgggcagaa
      361 cggatcggcg aagacgtaga caatgaccgg cgcatctttt ttaccgtcga ggatccagtg
      421 ggattgttcc atccgttgcc acatttcgcg tccggctggt gcgtaaattt ctttttcgat
      481 aagtgtgtta ctcaggtttt cacctttctc gttgtacatg taaccagaga tagcgtgctt
      541 accatcggga gtcaggtagg tagatggtga cgcccatatc ctgatacttt ccgagataac
      601 ctttcattcc tccgggggca tcgaacgttt tgatgattgt aatgccctgt ttttcaatcg
      661 cttttactgg ggaaggaagt tcctctgcga aggcgattgc aggaagcaga gccagtaaaa
      721 gtatcttttt taacattaat ttgtcctttt cagtcagtgc aaaagtcgag taaaaggcat
      781 aacctatcac tgtcataggt aagagcttag atcaggtgat tgccctttgt ttatgagggt
      841 gttgtaatcc atgtcgttgt tgcatttgta agggcaacac ctcagcctgc aggcaggcgc
      901 tgaagatacc aaagggtagt tcagattaca cggtcacctg gaaagggggc cattttactt
      961 tttatcgccg ctggcggtgc aaagttcaca aagttgtctt acgaaggttg taaggtaaaa
     1021 cttatcgatt tgataatgga aacgcattag ccgaatcggc aaaaattggt taccttacat
     1081 ctcatcgaaa acacggagga agtatagatg tccttgatta acaccaaaat taaacctttt
     1141 aaaaaccagg cattcaaaaa cggcgaattc atcgaaatca ccgaaaaaga taccgaaggc
     1201 cgctggagcg tcttcttctt ctacccggct gactttactt tcgtatgccc gaccgaactg
     1261 ggtgacgttg ctgaccacta cgaagaactg cagaaactgg gcgtagacgt atacgcagta
     1321 tctaccgata ctcacttcac ccacaaagca tggcacagca gctctgaaac catcgctaaa
     1381 atcaaatatg cgatgatcgg cgacccgact ggcgccctga cccgtaactt cgacaacatg
     1441 cgtgaagatg aaggtctggc tgaccgtgcg accttcgttg ttgacccgca gggtatcatc
     1501 caggcaatcg aagttaccgc tgaaggcatt ggccgtgacg cgtctgatct gctgcgtaaa
     1561 atcaaagcag ctcagtacgt agcttctcac ccaggtgaag tttgcccggc taaatggaaa
     1621 gaaggtgaag caactctggc tccgtctctg gacctggttg gtaaaatcta aatttccttc
     1681 gtctttcgcg ctaccaactc cggcgcgaaa tacttcggaa attcacctaa ttcttcgggt
     1741 gctgcggcac ccgattttct tccccgcacc atgatgcaag ctgcatccag gtagccgtag
     1801 aggccgcttg catgatgatg tttaaagccc aggagataaa catgctcgac acaaatatga
     1861 aaactcaact caaggcttac cttgagaaat tgaccaagcc tgttgagtta attgccacgc
     1921 tggatgacag cgctaaatcg gcagaaatca aggaactgtt ggctgaaatc gcagaactgt
     1981 cagacaaagt cacctttaaa gaagataaca gcttgccggt gcgtaagccg tctttcctga
     2041 tcaccaaccc aggttccaac caggggccac gttttgcagg ctccccgctg ggccacgagt
     2101 tcacctcgct ggtactggcg ttgctgtgga ccggtggtca tccgtcgaaa gaagcgcagt
     2161 ctctgctgga gcagattcgc catattgacg gtgattttga attcgaaacc tattactcgc
     2221 tctcttgcca taactgcccg gacgtggtgc aggcgctgaa cctgatgagc gtactgaacc
     2281 cgcgcatcaa gcacactgca attgacggcg gcaccttcca gaacgaaatc accgatcgca
     2341 acgtgatggg cgttccggca gtgttcgtaa acgggaaaga gtttggtcag ggccgcatga
     2401 cgttgactga aatcgttgcc aaaattgata cgggcgcgga aaaacgtgcg gcagaagagc
     2461 tgaacaagcg tgatgcttat gacgtattaa tcgtcggttc cggcccggcg ggtgcagcgg
     2521 cagcaattta ctccgcacgt aaaggcatcc gtaccggtct gatgggcgaa cgttttggtg
     2581 gtcagatcct cgataccgtt gatatcgaaa actacatttc tgtaccgaag actgaagggc
     2641 agaagctggc aggcgcactg aaagttcacg ttgatgaata cgacgttgat gtgatcgaca
     2701 gccagagcgc cagcaaactg atcccagcag cagttgaagg tggtctgcat cagattgaaa
     2761 cagcttctgg cgcggtactg aaagcacgca gcattatcgt ggcgaccggt gcaaaatggc
     2821 gcaacatgaa cgttccgggc gaagatcagt atcgcaccaa aggcgtgacc tactgcccgc
     2881 actgcgacgg cccgctgttt aaaggtaaac gcgtagcggt tatcggcggc ggtaactccg
     2941 gcgtggaagc ggcaattgac ctggcgggta tcgttgagca cgtaacgctg ctggaatttg
     3001 cgccagaaat gaaagccgac caggttctgc aggacaaact gcgcagcctg aaaaacgtcg
     3061 acattattct gaatgcgcaa accacggaag tgaaaggcga cggcagcaaa gtcgttggtc
     3121 tggaatatcg agatcgtgtc agcggcgata ttcacaacat cgaactggcc ggtattttcg
     3181 tgcagattgg tctgctgccg aacaccaact ggctcgaagg cgcagtcgaa cgtaaccgca
     3241 tgggtgagat tatcattgat gcgaaatgcg aaaccaacgt caaaggcgta ttcgcagcgg
     3301 gtgactgtac gacggttccg tacaagcaga tcatcatcgc cactggcgaa ggtgccaaag
     3361 cctctttgag tgcttttgac tacctgattc gcaccaaaac tgcataagaa gaagtaagat
     3421 tcacctgcaa ttgcttagcc gccggggtca aacctggcgg cttttttatg gcattaaaaa
     3481 gccctgccgg gatgttccag gcagggcggc ataaatactt ttatctgtta gcgaatgacc
     3541 agtacaggca gattggcgtg gcggatcacg ctcgaggcgt tagaacctaa cagatgggtc
     3601 gaaatcgatg ggttgcgaga accgataact acaacatcag cccccagttc ttctgccaac
     3661 tcattgactt catcccgcac gctaccaaaa cggacatgtt gtttaatgcg ggaaggatcg
     3721 atggtgaagt ggctgaccat cgtttgcaga cgttcttctg cttcatgttg cagatgctct
     3781 tcaaaacgac gcacatcagc ggcaaaacgg tgcaggctca ggctggctga cccgggtagt
     3841 acgtgaagta gatgaataac tccgtcatcc tgggcgagga attcagcgtg gcgaacagct
     3901 ttgtcgctca attccatttc aaatacatca actggcataa tgattgtctt atacataacc
     3961 ctttctccct gttaatcatg aacaaatcat tcgccatgat tataatattt atccctgata
     4021 tttgtctggt tctttttcct tacgaacggt ttctgtgatt aatatattct cactgaacac
     4081 caggaattct cccaaaacct gcggtaccgc ccgttttccc gctgtgatag ctacccttaa
     4141 agactgactc ttttttgaac tgtctctgga ggttgcatga aagcattgac ttatcacggc
     4201 ccacatcacg ttcaggtaga aaatgttccc gatccgggca ttgaacaggc tgatgatatt
     4261 attctgcgta ttacggcaac ggcgatctgt ggctctgacc tccatcttta tcgaggcaaa
     4321 ataccccagg ttaaacatgg cgatattttg ggtcatgaat ttatggggga agtcgttgaa
     4381 accggaaagg acgtaaaaaa tttgcaaaaa ggcgaccggg tggtaattcc gtttgtcatt
     4441 gcttgtggcg actgtttttt ctgtcgaatg cagcaatatg ccgcctgcga aaataccaat
     4501 gcgggtaaag gcgctgcgct caataaaaaa cagataccag ctcccgcggc attgtttggt
     4561 tatagtcacc tgtatggtgg cgttcctggt ggacaggcgg aatatgtccg cgtccctaaa
     4621 gggaatgtgg ggccgtttaa agtaccgcct ttgctttcag atgataaagc ccttttcctt
     4681 tctgatattc tgccaacggc atggcaggca gcaaaaaatg cgcagatcca acaaggttca
     4741 agcgttgcag tctatggtgc cggtccagtg ggattgttga caatcgcctg tgcacggttg
     4801 ctcggtgcgg aacagatttt tgttgttgat catcatccct accgcttgcg tttcgccgct
     4861 gaccgctacg gcgcgatccc gattaatttc gatgaagata gcgatccggc acagtcaatt
     4921 attgaacaaa cggcaggtca ccggggcgtg gatgcagtaa tagacgccgt cggttttgaa
     4981 gcgaaaggca gcaccacgga aacggtgctg actaacctga aactggaggg cagcagcggt
     5041 aaagcgttgc gccagtgtat tgcggcggtc aggcgtggcg gcattgttag cgtaccgggc
     5101 gtctacgctg gatttattca cggtttcctg tttggcgacg cctttgataa agggttgacg
     5161 tttaaaatgg gacagaccca cgttcacgca tggctgggtg aattattacc gttaattgag
     5221 aaaggattac tgaaaccaga agaaattgtt acccactata tgccgtttga agaggccgcc
     5281 cggggatatg agattttcga aaaacgtgaa gaggagtgcc gtaaggtgat tctggtaccc
     5341 ggtgcacaaa gcgcagaggc ggcgcagaag gcggtttcag gtctggtgaa tgcgatgccg
     5401 gggggaacaa tatgatcgtc aggagtggtt ttcgaggtaa aggacagcca tgacgataat
     5461 cgccgccata atcagaaatc ctatcaggat gtaaaatgct tctgccatgg ttattcccac
     5521 aaacgaaacg gaataatttt gcagcaaaag tgaacagtga gaaccaggaa aaattgctga
     5581 ttttgcgtaa agaggatgcg agtgcatcct ctgggcaaag cgagttatcg cttgtgcaga
     5641 tgggattaaa gcaggtagtc gccagcagct tctggctggt attcgagttc cagcacttca
     5701 aggtgggttg caacgccgcc cggaagttcc cagtgaatgg aatcgccaac gcgcagcccc
     5761 agcagtgcgg cacctaccgg agccataacg gaaagctgag tattgctatc ggtcattttt
     5821 gccggataca ccagcgtgcg cacacgcact tcgccatcgc taagattgcg gaatttaacc
     5881 cggctgttca ttgtcaccac gtcgtgtggc atctcttctg gcgaacacat ttgggcgcga
     5941 tccaactctg cgtttaacgc gtcggcgatt ggcaaaccag cataggcggg ttgctccagc
     6001 agaatatcga tgcgttcggc atccaggtcg ttaatgatga tagttggtct ggacattttt
     6061 actccatgtc gtcggtactg cgagtgtcgc agataaacat acccaaaaga aaaccctcac
     6121 cgtcaggcgg cgagggttta actcacatga tgatactgac tgttgctcac tctttgaagt
     6181 gatttgcgtc acattcaggg aattcctcaa tgcagcgcat tatgtataat tcttaatcgt
     6241 cacggtttat ggaagacgaa ttagcgtgtt ttgtaaatca gatgattaat aacccacttt
     6301 atcaatcaca aaggttttgc cacagttgcc tgggtgtggt tgtggcaaga atgagtttgc
     6361 agaaagcgga gcgttgatgg cgtcagcttt gatcgagatc tgaatttcag tcaaatacgc
     6421 agggttaccc tggcacgtta gcttaactgc tttcacgttc tcttttcccc agcttttggc
     6481 aaaggcggcg tcaaagtcac ggcggctcac tgttttaccg taattatccg caaggaattt
     6541 tccggcttcg ctctctttaa tttcttgatt caggcgtacc atcgtaccga agtatgcgtc
     6601 cggatcaaaa ccaaagcagg caccgtgttt ggcatattcg tagcgttcca ggcaggaacg
     6661 tccgccagct cctggcatga cttcacttag tttagcggcc gtttccagtg ataacccggt
     6721 ttccggcgat gaacacatcc ggctggcgtg cgcttctggt agattcggga ttgggcgagt
     6781 agcgcaaccg aagcgcatcc agcggcgttc atcaacacca cgggcagcaa ccgatttagg
     6841 caatcctggc cacagaccat gtacggtcag aaaatcagct ttgttggtcg tttcggtttg
     6901 taggcgacat tcatctcgtt cgttacgatt tcgatcgtgt tgactctggc aaaatccggt
     6961 ttgccaggag agggccagga catagcgatc aaaatcgcca tactgttttg cctgcaacgc
     7021 tacggcgttg gcagaagaga agggaagcag agaaaccgcg agcaacgcgg cgttacgcca
     7081 gaatgctttc ataatgggtg tggaactcat acatacactg aatactatct attaaatcat
     7141 aaaaaagccc gccaggtggg cttacctggc gggcgtgatg atttattcag cgtttggcga
     7201 acgtattagt tccacatggc gagaatcggc cagccaacca acagcaacat agagatgtaa
     7261 atcaccccga agattgcgcc aagacgccag taatcttttg atttcacata gccacagccg
     7321 taaataatca ccccaggacc ggttgcatac ggcgtcagac agcccatgat accgatagac
     7381 agcaccagca ggatacacag ttgttccatt ggtacgcccg gaataccttt accgacggcc
     7441 agaataaccg acagcatggt tgcggtgtgc gcagacaggc tggcaaacag gtagtgtgca
     7501 aagtagaaca ccagaaccag tacaatcacc gttgcgtttg gtgagaatcc ttccaggtgc
     7561 gtactcatgg taccggcgaa ccagtcaata aagccagaac gagtcaggcc gttagccatc
     7621 acaaccagag ttgccaggtt gaccagcgtg ttccatgcgc tgttatagcg ggtaatatct
     7681 ttccaaggca caacgtgcag ggccagcatt agcgaaactg ccagcagacc aactgcagta
     7741 gcattaatga cttcactgcc aaaaacccac aaacctaagc tgagcaatac aaggccaatc
     7801 agtgtccact ctctgcgtgt cagcgcaccc atggttttca gttcatcacc cgcccaggtt
     7861 gccacttctt cactgtgtgt gatttccggt ttgtacagca cgtaggaaag ccacggcgca
     7921 atgataagca agataacccc aaccggcagg aagcagagga accactgcaa ccagctaatc
     7981 tggataccgg caattttgct gacgaactcc agacccagca cgtttggtgc cgcaccggtg
     8041 acaaacatgg acgaactcag actggtacta atgaccatca tccacatcaa atagccgcca
     8101 atacgacgcg cggacggatc gttggggaat gatttaaaca gcggcggcag gtttttaatg
     8161 accggaaaaa ccgtaccccc ggtacgcgcg gtgttggacg gtgtaaacgg tgccagcaga
     8221 atgtcgataa tgacaatcgc ataacccaac gtcagcgtgc gtttgcccat gaatttcacc
     8281 aggaaaaggg caatgcgacg acctaacccg gaaacttcat accctaatgc aaaaataaat
     8341 gcgccaaata ccagccatac cgtggtgctg gaaaaaccag ccagacccca tttcagcgcc
     8401 tgtttttgcg cattaaacgc tgggtcagct aattctttgg catcaaagag caggtaatta
     8461 ctgccaataa cgcaaatagt aaccgcaata aaactgatcg ctgttgccgg aattggctcg
     8521 aggatcatgc cgacaatcat tgccacaaac acagcgaagt aatgccatgc ctgtggtggc
     8581 ataccgtcgg ggacagggat aagaaacatg acacccatca ccaccagtgg ggccaatagt
     8641 ttccatatat tatcttttgc taaagacata cgggttctcc gaaaattaat atttccaaat
     8701 ttatcaagtg cttaaataat taaatctgtg ctaaaaacca ggtaaggatc agcaggtcgg
     8761 cactgccgcc tggactgaga tttcgttcga tacactccct gtcgaactgc cggagataat
     8821 cgagatcggc gggggttcga atgccccctt tttgcaataa tgtttgcgcc tcgcgctgta
     8881 gccagcgcag gcccccctcg ccaccgcgcg atgcaacgtt ggtatcgccg ttgatcgcca
     8941 tcagtaggag caaggtatcg agcaatgcca gttcaggatc taacccctga tccagcagag
     9001 tgaggtaatg cggcaaggcg tgattgatca ccagtggata acccgcttcg gcttcaccgc
     9061 gtgcgccggt aaggccaagc tgctggtaca accgttgacc tgccgtcagt tgtgaattat
     9121 tggtacgcag ttctcgatcg gtcaggccac ggcagaaact tgccgccgta gaacaaacgg
     9181 ttgttggcgt taccggttgg ttgagttgaa gcaaacggcc aattgccgca catagtagcc
     9241 ctaaagaaaa aatgctgcct ttatgcgtgt ttacgcccgc agtggcgcgg aacatatcac
     9301 cttcgcaagc cataccaatt gggcgtaatc cgtggagtac cgcttctggt gctatttccg
     9361 cactacaggc accaaattca atgaaacggg gtagccagcc ctgaatcgcc agcgcgctgc
     9421 ggtggaaatc ttccagcgcc atatctttat gtgcaccgca gttaatccga tccacgaggc
     9481 ctggtttcgg tgacagattg acttcagtca gcatggcgcg ccagcccagc agggcgtact
     9541 catcgatcaa tgacgtcgca agctttgtgg ttttagttga cgttgcaggc atcgacatcg
     9601 ttcagcagtg cctccatgcg gttgagtaaa tcggtcagtt gatgggtttt tccacgcgcg
     9661 cagacggctg cgctttgctc gcacaacagg cagcggcgag gcggcagtga atagtcgcgg
     9721 cgggagagaa tttcgccttc gggcgtcagg acatcgatat cccataaccg cccgagagga
     9781 tgactatgtt caagctcaat ggtggcgagc ttgaggtcgc gagccggggc ggcaatgctc
     9841 aacatgccct ccggcccgct ggcggaaacc agtgcagcct gctcctgaat ttgccagccc
     9901 tgttttgcgg ctaaggcacg caaggctgtc acgccatgat taaaaattcg gcgtgtgacc
     9961 tcgctgtctt taatcggccc aggcgcaacc acggtaaagg agaccagtgg aacaggatgg
    10021 cgcttgagcc agacgtgttg ccgtgcttgc ctttcatccc ggctgacgag cagctcggga
    10081 attgacaccg catggtggct ggcgagttca ggaagcaggt gcatggctta ttccttcacc
    10141 tgatgcacaa catcgatcac cgagccatcg cggtaacgca caacggcaac gacgcggtcg
    10201 gtgaattcaa tcggctgtgg ttcaccggtc agcagacgcg cgcgttcgcg cagccactca
    10261 atggaaacca ctttaatgcc cgcttcctgc agacgttctg ccagttccgg acgtgccggg
    10321 ttaactgcga taccgtggtc tgtgaccaga atatcgacac tggagcctgg ggtgatgcag
    10381 gtcagcacgt tatccaccag agtcggaata cgaccgcgta ccagcggcgc gacgatgatg
    10441 gaaagcgcag aggcaatcgc ggtatcgcag tgaccaccgg aagcaccacg cagtacgccg
    10501 tcagagccgg tcagcacgtt aacgttgaac tgggtgtcaa tttccagcgc gctcagtacc
    10561 accacgtcga gacgatcaac cgatgcgcct ttcgaacccc agttagcgta ctggttggcg
    10621 ctgatttcga tgtgattggg gttacgggcc agcgattgcg cagcatggct gtcaaagctc
    10681 tgcacatcca gcagtttgcg gatcagacct ttttcgtgca gatcaaccat cgtcgcggta
    10741 ataccgccga gggcgaagtc ggcgcgaata tcgcggctac gcattttgtc ttccaggaaa
    10801 cgggttaccg ccagcgatgc gccgccggtg ccggtttgca tggagaaacc ttctttgaag
    10861 tagccagagt tgacaatcac atccgcagcg ctacgggcaa taagcagttc gcgcgggtta
    10921 gtggtcatac gggtcgcgcc agcgccgatt tttgcagcat cgccaacgcg gtcaactttg
    10981 acgatcaaat caacctgatc ttgctcaatg cttgccggat tatgcggata aggcagcagt
    11041 tcttcggtaa gcatcacaac ctgttttgcg ttgtcggcat caaccattgc atagccgagg
    11101 gagccgcagc aggctttacc ggtgtaaccg ttggcattac cgaattcatc acaggacggg
    11161 acgccgagga aagccacgtc gatattcagt tcgccgctct gtaccagatg cacacgtccg
    11221 ccgtgagagt ggatttgcac cggttccgca agcagaccac gggaaatttc ttctgccagt
    11281 ggaccgcgca ggccggaggt ataaatgcgg gtaaccacgc cctggcgaat gtgttctacc
    11341 agtggcgcat ggcaatcact cagggagctg gacgccaggg tcaggttttt aaagcccatc
    11401 ttagcgatga cgtccatcac catattgacg gtcaggtcac cgccacggaa agcgtgatgg
    11461 aaggaaaccg tcatgccgtc ctgtaaacca gagcgacgaa tcgcttcttc ctggttggcg
    11521 cacagtttgc gatcgcgcgc tttttcagcc tagtaggttt gctttggtga gttctggaaa
    11581 gcggcaagat cgcattcagc gcgacgattc caggccgcta cccgttcttg tcgttgagat
    11641 tgttcaattt tctgcgtcat tttgattgcc ttattcttcg cggatgccgg aaagttctgc
    11701 acgggagagc accagacggg cacgatcgat aaccggaccg tccaccatct tgccgttcag
    11761 ggaaaccacg ccgaggcctt cgcgagcggc ggcttcagcg gcttctacga cgcggcgggc
    11821 gtgatccact tctttctggg tcggtgcgta gaggttgtgc aacagatcaa tctgacgcgg
    11881 gttgatcagc gatttgccgt caaagcccag ctgtttgatg tgggcggctt cttgcaggaa
    11941 tccggcttcg ttgttagcgt cggaatagac ggtatcgaac gcctgaatac ccgcagagcg
    12001 cgcggcctgc aaaatggaac agcgtgcgaa cagcagttca gttccttccg gggagcgttc
    12061 tgtacgcagg ttgcgcacat agtcttctgc accgagggcg ataccgatca aacgctcgga
    12121 agcgtgagcg atttccactg cgcgggtaat ccccagcgga gattcaatcg ccgccagcag
    12181 gccggtgctg ccgggttcac gaccacaggc tttttcgata cgcaggatct ctttttcgat
    12241 atccagaaca tcctgagcgg tatcggtttt cggcagacgc acaacgtccg caccaccgcg
    12301 aacgacggct tccaggtcgt tgacacccca ttcggaatcc agcgcgttga cacgcacaat
    12361 ggtttcaata tcgcgataca gcggatgttg cagcgcgtgg taaaccatgc ggcgggcggt
    12421 gtctttttca cgcaatgcta cggagtcttc gaggtcaaac atcagggcat cagccgggta
    12481 gatgaaggag ttgctgacca tcgcggcatt ggcaccaggc acaaacaaca tgctgcggcg
    12541 ggtgcgagtt ttacgttgtt gcagcgaagc ggaaatcatt ggcaatcctc ccatggcaga
    12601 gccgggatac cgctggcgcg tgccagcagg gcttccagtc gtgcacgtaa aatgcagtcc
    12661 agtgcgcctt tgtcatcgac attcagctgt acgccgcgca cgttgtagcg ggcgagaacg
    12721 tccagaatgg tggtgcgaat tgcatcgcca aactgtttct caacgctgct attgatttgc
    12781 aggtcgatat cctgcgtatc gagtggggcg atgcgtatca tcacatcccc agactcaagg
    12841 gtgcctgcaa cggcgggctg gtttattttc atttttcacc tgtttctcat gcgggggtct
    12901 tttgacgagc tgccgcgtcc tggcgggagt gctcaagcag gttctgcaaa taatgcagcg
    12961 tgactgcagg gaccagcggc gcgatagccg tgagatcgtt tttcgccagc agttgacgta
    13021 cccgggaagc ggatatcggc atctcctggt aacgcagccg ctcaatttca accagttcga
    13081 tgggcggtgc ggagatagtc ggcgtttcca gccagtagcg catatcctgg ttgtactggg
    13141 cggtaacgcg acaaaagggt tcagtaccga caaagcggtg agttacaccc agcgcgggag
    13201 cgaggtactg acggaaaatc ttcagatcaa tttcggtgta acaatggtta atgacgctct
    13261 gttctttaat gaagtagcaa gggaacgtag cgcgggagat gatgtattcg gagccgcgat
    13321 gcacagtcag gcgtggaata tcggcggtgc cttttaacac cagatccaac cggtcttcat
    13381 aggggaagcg tgaagaatct tctttgacta aaaacagatg caaccagtcg cactgtgccg
    13441 cagcttgttg aatcagataa cggtgaccat tcgtgaaggg attggcgttc atcacaatgc
    13501 agccaatctt gttccctgga tgacgaaatt ttttcagcga ttcggcatag cgtttcagcc
    13561 gcgtggcgct gttttccatc agcaccatca cgccgggtac gctggtcagc gtggaaaaac
    13621 cgcactggcg gaacagcgcc tcgtattcgg ttttggtata aataaacaga tgcgtgctgt
    13681 gccgctcata ggcgaggttt atcaattcag tggctaatgt cagcgccagt ccttcaccgc
    13741 gaacggattc actgatagca acgcatttaa taatatttcc ggcaattcca ccgcacgtga
    13801 taagcttttc atcgcgggtt acggtaataa atacttcgac tgtggtgtca acg
//
LOCUS       DAESHI010000114        13612 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.115, whole genome shotgun sequence.
ACCESSION   DAESHI010000114 DAESHI010000000
VERSION     DAESHI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13612
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.115"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            196..351
                     /gene="rseD"
                     /locus_tag="KW011_003383"
     CDS             196..351
                     /gene="rseD"
                     /locus_tag="KW011_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HBH7661643.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            348..923
                     /gene="rpoE"
                     /locus_tag="KW011_003384"
     CDS             348..923
                     /gene="rpoE"
                     /locus_tag="KW011_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HBH7661644.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            956..1606
                     /gene="rseA"
                     /locus_tag="KW011_003385"
     CDS             956..1606
                     /gene="rseA"
                     /locus_tag="KW011_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HBH7661645.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            1606..2562
                     /gene="rseB"
                     /locus_tag="KW011_003386"
     CDS             1606..2562
                     /gene="rseB"
                     /locus_tag="KW011_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HBH7661646.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            2559..3038
                     /gene="rseC"
                     /locus_tag="KW011_003387"
     CDS             2559..3038
                     /gene="rseC"
                     /locus_tag="KW011_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HBH7661647.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3236..5035
                     /gene="lepA"
                     /locus_tag="KW011_003388"
     CDS             3236..5035
                     /gene="lepA"
                     /locus_tag="KW011_003388"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBH7661648.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5051..6025
                     /gene="lepB"
                     /locus_tag="KW011_003389"
     CDS             5051..6025
                     /gene="lepB"
                     /locus_tag="KW011_003389"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBH7661649.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            6298..6978
                     /gene="rnc"
                     /locus_tag="KW011_003390"
     CDS             6298..6978
                     /gene="rnc"
                     /locus_tag="KW011_003390"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBH7661650.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            6975..7880
                     /gene="era"
                     /locus_tag="KW011_003391"
     CDS             6975..7880
                     /gene="era"
                     /locus_tag="KW011_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBH7661651.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            7892..8620
                     /gene="recO"
                     /locus_tag="KW011_003392"
     CDS             7892..8620
                     /gene="recO"
                     /locus_tag="KW011_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBH7661652.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            8632..9363
                     /gene="pdxJ"
                     /locus_tag="KW011_003393"
     CDS             8632..9363
                     /gene="pdxJ"
                     /locus_tag="KW011_003393"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HBH7661653.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            9363..9743
                     /locus_tag="KW011_003394"
     CDS             9363..9743
                     /locus_tag="KW011_003394"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBH7661654.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            9965..10045
                     /gene="shoB"
                     /locus_tag="KW011_003395"
     CDS             9965..10045
                     /gene="shoB"
                     /locus_tag="KW011_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HBH7661655.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(10239..10499)
                     /gene="yfhL"
                     /locus_tag="KW011_003396"
     CDS             complement(10239..10499)
                     /gene="yfhL"
                     /locus_tag="KW011_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HBH7661656.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            10714..11904
                     /locus_tag="KW011_003397"
                     /pseudo
     CDS             10714..11904
                     /locus_tag="KW011_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(12089..13276)
                     /locus_tag="KW011_003398"
     CDS             complement(12089..13276)
                     /locus_tag="KW011_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBH7661657.1"
                     /translation="MLPRFADIFQQGNHWLNWLEKQPERAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLTEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNAPHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(13276..>13612)
                     /locus_tag="KW011_003399"
     CDS             complement(13276..>13612)
                     /locus_tag="KW011_003399"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661658.1"
                     /translation="KPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDSFDDAVECCAD
                     LNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGGMYCPECGTVH
                     WPDGVAPPF"
BASE COUNT         3443 a         3398 c         3527 g         3244 t
ORIGIN      
        1 acacctgcta ttttaatatt tgttacagtg gctaaacacg ttgacgcagg gcggcgagaa
       61 aaagagaagt tactggctgg tggaggatta ggtggtgaaa taaaaaggcc gttgggttac
      121 tcttcaggca gttaaatggg catttctaca cagataatgc gatgttcaga ttctgtagac
      181 ttataatgat agataatgat ccgtctacag catgacaaac aaaaacagat gcgttacgga
      241 actttacaaa aacgagacac tctaaccctt tgcttgctca aattgcagct aatggagtgg
      301 cgtttcgata gcgcgtggaa atttggtttg gggagacttt acctcggatg agcgagcagt
      361 taacggacca ggtcctggtt gaacgggtcc agaagggaga tcagaaagcc tttaacttac
      421 tggtagtgcg ctatcagcat aaagtggcga gtctggtttc ccgctatgtg ccgtcgggtg
      481 atgttcccga tgtggtacaa gaagctttta ttaaagccta tcgtgcgctg gattcgttcc
      541 ggggagatag cgctttttat acatggctgt atcggattgc tgtaaataca gcgaaaaatt
      601 acctggttgc tcaggggcgt cgtccacctt ccagtgatgt ggatgccatt gaagctgaaa
      661 acttcgaaag tggcggcgcg ttgaaagaaa tttcgaaccc tgagaactta atgttgtcag
      721 aagaactgag acagatagtt ttccgaacta ttgagtccct cccggaagat ttgcgcatgg
      781 caataacctt gcgggagctg gatggcctga gctatgaaga gatagccgct atcatggatt
      841 gtccggtagg tacggtgcgt tcacgtatct tccgagcgag ggaagctatt gataacaaag
      901 ttcaaccgct tatcaggcgt tgacgatagc gggatactgg ataagggtat taggcatgca
      961 gaaagaacaa ctttccgctt taatggatgg cgaaacgctg gatagtgagc tgcttaacga
     1021 actggctcat aacccagaaa tgcagaaaac ctgggaaagc tatcacttaa tccgtgattc
     1081 aatgcggggt gatactcccg aggtgctcca tttcgatatc tcttcacgcg tgatggccgc
     1141 cattgaagaa gagccagtac gtcaaccggc gacattgatc ccggaagccc agcctgcgcc
     1201 gcatcaatgg cacaaaatgc cattctggca gaaagtacgt ccgtgggcgg cacagcttac
     1261 ccaaatgggc gtagcagcat gcgtatcgct tgcagttatc gttggcgtcc agcactataa
     1321 tggacaatct gaaacgtccc agcagcccga aacgccggta tttaatacac tgccgatgat
     1381 gggtaaagcc agcccggtaa gcctgggagt accttctgaa gcgaccgcaa acaatggtca
     1441 acagcagcag gtacaggagc agcgtcgtcg cattaatgca atgttgcagg attacgaact
     1501 gcaacgccga ctccactctg aacagcttca gtttgagcag gcgcaaaccc agcaagccgc
     1561 tgtacaggtg ccaggacttc aaactttagg aacgcaatcg cagtaatgaa gcaactttgg
     1621 tttgccatgt cattagtgac aggtagcctg ttattctctg ctaacgcctc ggccactccc
     1681 gcgtccgggg cgttattaca gcagatgaac ctggccagtc agtcactgaa ttacgagctg
     1741 tcattcatca gcatcaataa acagggtgtt gagtctctgc gttatcgaca tgcacgcctc
     1801 gataaccgtc ctcttgcaca attgttgcaa atggatggcc cgcgccggga agtggtacag
     1861 cgcggcaatg aaatcagcta ttttgaaccg ggacttgaac cgttcacgct taatggcgat
     1921 tacattgttg attctctgcc atcgcttatc tataccgatt tcaaacgcct ttctccttac
     1981 tacgacttta tctccgtcgg gcgcacgcgt attgctgatc gtctttgcga agtcattcgc
     2041 gtggttgccc gagatggtac acgctacagc tacatcgtgt ggatggacac tgaatcgaaa
     2101 ttaccgatga gggttgatct ccttgatcgc gatggtgaaa cgctggaaca atttcgcgtg
     2161 atttctttta acgtcaatca ggatatcagc agcagtatgc agacgctggc gaaggcaaat
     2221 ttgccgccgt tgctttctgt tcctgtaggt gaaaaagcta aattcagctg gacgccaacc
     2281 tggttgccac agggttttag cgaagtttcc agtagtcgac gtccgctacc gacgatggac
     2341 aacatgccta tcgaatcacg tctctattcc gacggattat tcagcttctc ggtaaacgtt
     2401 aaccgcgcta cgccatcgag caccgatcag atgttgcgca ccggacgcag aaccgtcagt
     2461 acaagcgtac gtgataacgc cgaaatcacc attgtcggtg aactgccgcc gcaaacggcg
     2521 aaacgcattg ccgagaatat taagttcggg gcagcgcaat gatcaaagag tgggctaccg
     2581 tcgtctcctg gcaaaacggg caggcgctgg tcagttgtga tgttaaagcc tcatgcagca
     2641 gctgcgcttc acgcgccggt tgcggtagcc gcgtgttgaa taaacttggc ccgcaaacca
     2701 cgcataccat tgtcgtaccc tgtgatgaac cgttagtgcc ggggcaaaaa gtggaattgg
     2761 ggatcgccga aggcagcctg cttagctccg cattactggt ttatatgtcg ccgctggtgg
     2821 gattattcct tatcgcttcg ctatttcagc tactctttgc ttctgacgtt gcagcattat
     2881 gtggtgcgat ccttggcggt attggcgggt ttctgattgc gcgcggttac tcgcgtaagt
     2941 ttgctgcccg ggcagaatgg caaccgatca tattaagcgt ggcattgccg cctgaccttg
     3001 tgcgatttga aacatcttcg gaagacgcga gccagtgatt tcactggctt tgttgcattt
     3061 tgcataacaa atgcgaggac gtttccagat accacaatcc atacggtatg aacatttcct
     3121 cgcctcaatg ttgtagtgta gaatgcggcg tttctattaa tacagacgtt aagctcagaa
     3181 cagcgacttc taaaagcctg gttaaccggg cattaaggca caataatcat actttatgaa
     3241 gaatatacgt aacttttcga tcatagctca cattgaccac ggtaaatcga cgctgtctga
     3301 ccgtattatc cagatctgcg gtggcctgtc tgaccgtgaa atggaggcgc aggttctcga
     3361 ttccatggat cttgagcgtg agcgtggcat taccatcaaa gcgcaaagcg tgacgctgga
     3421 ctacaaagcg tctgacggcg aaacctatca gcttaacttt atcgacaccc cgggccacgt
     3481 agacttctcc tatgaagttt cccgttcgct ggctgcctgt gaaggtgcat tgctggtggt
     3541 cgacgccggg cagggcgtgg aagcgcaaac cctggcaaac tgctacaccg ccatggaaat
     3601 ggatctcgaa gtggtgccag tactgaacaa gattgacctg ccggcagccg atcctgaacg
     3661 cgtggcggaa gaaattgaag atatcgtcgg catcgacgcc actgacgcgg tgcgctgttc
     3721 agcgaaaacc ggcgttggcg tgcaggacgt tctcgaacgt ctggtgcgcg acattccgcc
     3781 gccggaaggc gatccggaag gcccgttgca ggcactaatt atcgactcat ggttcgacaa
     3841 ctacctgggc gttgtttcac ttatccgtat taaaaacggc accctgcgta agggcgacaa
     3901 agtgaaagtc atgagtaccg ggcagaccta taacgccgac cgtctgggca tcttcacgcc
     3961 gaaacaggtt gaccgcactg aactgaaatg tggcgaagta ggctggctgg tatgtgcgat
     4021 taaagatatc cacggcgctc cagtcggcga taccttaacg ctggcgcgta atccggcaga
     4081 aaaggcgctg cctggcttta agaaagtcaa accgcaggta tacgccggtc tgttcccggt
     4141 aagttccgac gactatgaag ccttccgtga cgcgctgggt aaactcagcc tgaatgatgc
     4201 ctcactgttc tatgagccgg aaagctccag cgcgctgggc tttggtttcc gctgcggctt
     4261 ccttggcctg ctgcacatgg agatcatcca ggaacgtctg gaacgtgaat acgatctgga
     4321 tctgatcacc actgcgccga ccgtagtgta tgaagttgaa accacgtcaa gggaagttat
     4381 ctacgtcgac agcccatcca agctgcctgc ggtaaataac atttacgaac tgcgcgagcc
     4441 gattgcagag tgtcacatgc tgctgccgca ggcatatctc ggcaacgtta ttacgctgtg
     4501 cgtagaaaaa cgcggcgtgc agaccaatat ggtttaccac ggtaatcagg tggcgctgac
     4561 gtacgagatc ccgatggcgg aagtggtgct cgacttcttc gatcgcctga aatctacctc
     4621 gcgtggttat gcgtctctgg attacaactt caaacgcttc caggcgtccg acatggtacg
     4681 tgtagacgta ttaatcaacg gtgaacgtgt tgatgcgttg gcgctgatca cccaccgtga
     4741 taattcgcaa aaccgcggtc gcgagttggt ggagaagatg aaagatctga tcccacgcca
     4801 gcagtttgat atcgccattc aggcagcgat tggtacgcac attattgcgc gatccaccgt
     4861 gaaacagctg cgtaaaaacg tactggctaa atgttatggc ggcgatatca gccgtaagaa
     4921 aaagctgctg cagaagcaga aagaaggtaa gaaacgcatg aagcagatcg gtaacgtcga
     4981 gctgccgcag gaagcgttcc tcgccattct gcacgtcggc aaagacaaca aataaccctt
     5041 aggagttggc atggcgaata tgtttgccct gattctggtg attgccacac tggtgacggg
     5101 cattttatgg tgcgtggata aattcttttt cgcacctaaa cggcgggaac gtcaggcagc
     5161 ggcgcaggcg gctgccggtg actcactgga taaagcaacg ttgaaaaagg ttgcaccgaa
     5221 gcctggctgg ctggaaaccg gagcttctgt ttttccggtg ctggctatcg tattgattgt
     5281 acgttcgttt atttatgaac cgttccagat cccgtcaggt tcgatgatgc cgactctgtt
     5341 aatcggtgat tttattctgg tagagaagtt tgcttatggc attaaagatc ctatctacca
     5401 gaaaacgctg atcgaaaccg gtcatccgaa acgcggcgat atcgtggtct ttaaatatcc
     5461 ggaagatcca aagcttgatt acatcaagcg cgcggtgggt ttaccgggcg ataaagtcac
     5521 ttacgatccg gtctcaaaag agctgacgat tcaaccggga tgcagttccg gccaggcgtg
     5581 tgaaaacgcg ctgccggtca cctactcaaa cgtggaaccg agcgatttcg ttcagacctt
     5641 ctcacgccgt aatggtgggg aagcgaccag cggattcttt gaagtgccga aaaacgaaac
     5701 caaagaaaat ggaattcgtc tttccgagcg taaagagaca ctgggtgatg tgacgcaccg
     5761 aattctgaca gtgccgattg cgcaggacca ggtggggatg tattaccagc agccagggca
     5821 acaactggca acctggattg ttccgccggg acaatacttc atgatgggcg acaaccgcga
     5881 caacagcgcg gacagccgtt actggggctt tgtgcctgaa gcgaatctgg tcggtcgggc
     5941 aacggctatc tggatgagtt tcgataagca agaaggcgaa tggccgacag gtgtgcgctt
     6001 aagtcgcatt ggcggcatcc attaatagcc catcttcgtt cacgttgtcg ccgttatggc
     6061 gacaacgtga attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt
     6121 gtatagaata ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa
     6181 cagcgttggt ccccatatac cggtaaactg aaactgcagc gaagcagtta gcagagccat
     6241 gtatatcagg tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg
     6301 aaccccatcg taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa
     6361 ctgttgcagc aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa
     6421 tttttaggcg actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct
     6481 cgtgtggatg aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg
     6541 gcggaactgg cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt
     6601 aaaagcggtg gatttcgtcg tgagtcaatt ctcgccgata ccgtcgaagc attaattggt
     6661 ggcgtattcc tcgacagtga tattcaaacc gtcgagaaat taatcctcaa ctggtatcaa
     6721 actcgtttgg acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa
     6781 gaatatttgc agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc
     6841 gaagcgcacg atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg
     6901 gttggcacag gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa
     6961 aaactggagc tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac
     7021 gtccgaacgt tggcaaatcc acattgttga acaaactgct ggggcagaag atctccatca
     7081 cctcccgcaa agcgcaaaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt
     7141 atcaggcgat ctacgttgat acccctggcc tgcatatgga agaaaaacgc gctattaacc
     7201 gcctgatgaa caaagcggcg agcagctcta ttggcgacgt tgagctggtg atttttgtcg
     7261 ttgaaggcac ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgatggca
     7321 aagcgccggt gatcctcgcg gtgaacaaag tggataacgt gcaggaaaaa gccgatctgc
     7381 tgccgcactt acagttcctg gcaagtcaga tgaacttcct cgatatcgtg ccgatctctg
     7441 ctgaaaccgg gctgaatgtc gacactatcg cagcaatcgt gcgtaagcat ctgcctgaag
     7501 cgacccatca cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg
     7561 aaatcatccg cgaaaaactg atgcgtttcc tcggcgctga actgccgtac tccgtgaccg
     7621 tggagatcga acgtttcgtc tctaacgaac gcggtggtta tgacatcaat ggtttgattc
     7681 tcgttgagcg tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa
     7741 ccatcgggat tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg
     7801 aactgtgggt aaaagtgaaa tccggttggg ccgacgacga acgcgcactg cgcagtctcg
     7861 gttacgttga cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct
     7921 gcatagtcgc ccgtggagcg aaaccagcct gatgctggac gtcttcacgg aggaatcggg
     7981 tcgcgtgcgt ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt
     8041 acagcctttc acccctctct tgctacgttt cggcgggcgt ggcgaagtca aaacgctgcg
     8101 cagtgctgaa gctgtctcgc tggcgctgcc attaagcggt atcacgcttt acagcggtct
     8161 gtacatcaac gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt
     8221 tttcgattac ttgcactgca ttcagtctct tgcaggtgcc actggtacgc cagaacccgc
     8281 gctgcgccgc tttgaactgg cacttctcgg gcatctgggc tatggcgtca attttaccca
     8341 ttgcgcgggt agcggcgagc cggtagatga caccatgacg tatcgttatc gcgaagaaaa
     8401 agggtttatc gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc
     8461 gttaaacgcg cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg
     8521 catggcgctt aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca
     8581 gtttatgcct aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa
     8641 ttactgttag gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct
     8701 tacccggatc cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc
     8761 gtgcatttac gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag
     8821 acgctggata cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc
     8881 gttgagacga agccacattt ttgctgcctg gtaccggaaa agcgtcagga agtaacaacc
     8941 gaaggcggcc tggacgttgc agggcagcgt gaaaaaatgc gcgatgcctg caaacgtctg
     9001 acagatgccg ggattcagat ttctctcttt atcgacgccg atgaagagca gatcaaagct
     9061 gcggcagagg ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa
     9121 actgacgccg aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct
     9181 agtctcggtc tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt
     9241 gccgccatcc ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg
     9301 atgaccggac tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc
     9361 taatggcaat attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga
     9421 tcgcccgatc cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct
     9481 ggaaaacgca ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg
     9541 cagcaaaagc gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat
     9601 tcaatgatga gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg
     9661 aaaagctcgg cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg
     9721 ccacggtaat tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa
     9781 ctggtaagga acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac
     9841 aacagcgatt atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa
     9901 aaactcaagc ctttacgaag aaagcaatac tgaacggctg cattccttgt ttaaggaaaa
     9961 acgaatgact gattgccgat acctgattaa acgggtcatc aaaatcatca ttgctgtttt
    10021 acagctgatc cttctgttct tataacacaa ggaaacgtac ttaaggtgcg tccggtgaac
    10081 caatcggacg cgcctttaat aactataaat aagtgtcttg gcagatacta tataaattaa
    10141 cttagtgaat gattatgcta atgtcatcaa ttaaataaat ataatggcgt taaggcttcc
    10201 cagtaatata attaatactc tacttccaga gtagaatatt aaattttatc cgcgtggtgc
    10261 atcagcacaa atttatccca caactgttct tctgtctcga catgcgccgg atctttcaca
    10321 atagtattgg ggatcgggca caccttctgg caggttggtg tctcgtagtg ccctacgcat
    10381 tcggtacact tatcgctgtt aatctcgtag atatgatctc ccattgaaat cgcctcattc
    10441 gggcattcgg gttcacacat atcacaatta atacagcgtt tagtaattag taaagacatt
    10501 tcaatggatt accgttaaat cattttaaaa tcagtaagtt gtatcgagtt tgtatgcttt
    10561 actgtcatta acttactgta tgttgatcca gtgtatttaa ccttgataaa ctcagtccag
    10621 caacacaaaa ccgcaacaca ttgcattttg tcccgtagaa aagacttgta tgtgtgagct
    10681 tgttttctgc gcctacgcag ataaggattg agaatgccgc gcactgtaac acataatccg
    10741 gatagcccca ataatgacga tgttttagcc gcatctgaaa aatgggacgc ctgtaaaccc
    10801 ccctatacca gcgcacacat gaaaatctgt gttgctgccg ccaaaatcat cctcgctgct
    10861 tccggcgtgg ctcgccgttc caaatacgaa aaagagaact atctccgtat cgatttcagc
    10921 aaagccggta aggttacatt ttacgccgag tttccaaaaa agatgggcct caaaggtaaa
    10981 aagctcggcg agtggccgga gctcgctatc cagctggcgc gcgaaaaagc actaggtatg
    11041 gctgacggtg gactgcgggc agagtccgta catgcagcgc tggaaatgta ccgggatgac
    11101 ctcaaagcca aagtagcccg gcagaagctg agcccggaca gtttcacaac ctacggggtg
    11161 cgtatcgacc ggattaaagc aacgttcggc gagcgtgagg tgttcagcga cgtaacatac
    11221 aatcggctgg tggaagtgct ggacgagtgg atcgccactc gctcgaacaa taacgccctg
    11281 gagttgtttg ccgagctccg tcggttctgg aagttctgcg cacctactct ttgcaacggc
    11341 cgcaatgttg ccgccagtct gccagatgat tatgtttcct cccgcgtaca gaaacctacc
    11401 cccacacggc tttttaccga tattgaatca atcgcccgac tctggctcaa tgttgctgcc
    11461 tgcacctctg tacaccagaa gaatgctgtt cgcttcatga tcatcactgg tgttcgtccg
    11521 attaatgtcc ataacctgcg ctgggactac gtttacgagg aggctggtga aattgtttat
    11581 ccggaagggg ttatcggcat gcgaggggct atgaaaacac aaaaggcttt ccgcctgccg
    11641 ataacgcctg agatccggcg gattatcgac gagcagaaag cctggcgtga ttcagttcct
    11701 gagtgcaaca gggattatgt atttttgcag ccacgtgatc caatgcagcc attttcaaaa
    11761 cgatcactgg ataagctggt gaaaacatac agcccggacg gggctgtaaa aggaataaaa
    11821 catgatggga ctgttaaagg gaaagacggt gcatttaata cgatgtgccg taaattcctt
    11881 aagagcaatg ttattgcctt gatcgatagg aattaaaacc ccaaaaagat taaaaaaaca
    11941 ccacaaaacg gatgtttctt caacaccact tttgctcata tgaacggaac cgacgattaa
    12001 actggatggc tctgattgat tcagggtatg aatggcggtt ttttgctccg tttccctcaa
    12061 aatggacgca actttccctc tgcggctctc agccgcacca ccgcatccgg gccagcggct
    12121 catgcatcag gacctgctct gccagacggt agccccgctt cagccccgta aaacgcatct
    12181 gactcccgca cagcacgcac ttcagcgggt caaccttcag taacctctga tacatccctc
    12241 tccaggtgat ttgcatcgcc gtttttctca ctgtctccgt tatgatgtac accacttctt
    12301 ccagtaaccg ccgtttcgcc ggactcaaaa aaccgtagta cctcaccata cggaacccct
    12361 tatccgccac atgccaggag aacctttcca tgaactcatc tccactcatc aacaggtatt
    12421 cttcccgttt tgttcggtga ctgttgtaac gcagaccgat ttcatcctga ccggcataat
    12481 gctccagacg actcatcggc accggtggct ttttcaggta agagccaaag tacaccgcca
    12541 catggggggc attatccatc acccgggata cgttgacatt ccagccacgg cggtaatgcg
    12601 tgtccaggaa gcgattccat tcccgtttac tgcttccttc tgctgccagc gcatccggca
    12661 tcaccaggtc agggtatttc cgtgacagca accgtgttat ccggtagcgc cacatgctca
    12721 tcaccttacg ggcgtaaaaa tgaagatttt tccaggtgtg gcccgacgtc acaccaccgg
    12781 cagttgtcga taaatggata tgcggatgcc actgctggtc acgcccccat gtgtggatca
    12841 ccgtgaatat ccccggctcc acatctgcct gatggcagat ttccagtatc acatccgctg
    12901 caatgcggct catctctgtc agtaaccacc ggttgtggaa caccagggac cagtactggc
    12961 agggaagtgt gaacacaata tgctgccacg ggcagtcggg gaccaggctc agcagatact
    13021 gtatccactg tgcgccagcc ttcaccccgc agtgcgggca ggagcggctt ttacaccgga
    13081 agcagacctt ttttgtatgg caacagtccg gtgatgaaca gcaccactgt gtatacccca
    13141 tcagtgtggt cccgcacgcc atgattttgg tcaccgactc aatcaccacc ggacgtactg
    13201 ccctttccgg ctgcttctcc agccagttaa gccagtggtt tccctgctga aagatatcgg
    13261 caaaacgggg aagcatcaga agggcggggc gactccgtcc ggccagtgaa ccgtgccaca
    13321 ctccgggcag tacataccgc cggcgctgat accggaaaga atggtcgcaa attcccgctc
    13381 cgtgcagcgg gcgatttccg gatacccttc gtcatcaaca cgtacaaacc agaagaccag
    13441 ctttttgttt cccgcatcca caaagaacgg aatattcagg tctgcgcagc attcaacggc
    13501 atcgtcaaaa ctatcaaagc gcagaacttc tgcgtcttct tcgtcaaaaa aatcatcttc
    13561 gtgaagcttc acgacatagc ggggaagttt gcttctttga gaggcgggtt ta
//
LOCUS       DAESHI010000115        13570 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.116, whole genome shotgun sequence.
ACCESSION   DAESHI010000115 DAESHI010000000
VERSION     DAESHI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.116"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..183)
                     /locus_tag="KW011_003400"
     CDS             complement(<1..183)
                     /locus_tag="KW011_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661659.1"
                     /translation="MTAFRAAFKAYRMHQVLILPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSA"
     gene            complement(225..762)
                     /locus_tag="KW011_003401"
                     /pseudo
     CDS             complement(225..762)
                     /locus_tag="KW011_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(1123..1224)
                     /locus_tag="KW011_003402"
                     /pseudo
     CDS             complement(1123..1224)
                     /locus_tag="KW011_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(1508..2479)
                     /gene="uxaB"
                     /locus_tag="KW011_003403"
                     /pseudo
     CDS             complement(1508..2479)
                     /gene="uxaB"
                     /locus_tag="KW011_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
     gene            complement(2706..3656)
                     /locus_tag="KW011_003404"
     CDS             complement(2706..3656)
                     /locus_tag="KW011_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH7661660.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3795..4154)
                     /locus_tag="KW011_003405"
     CDS             complement(3795..4154)
                     /locus_tag="KW011_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HBH7661661.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(4154..5080)
                     /gene="glsB"
                     /locus_tag="KW011_003406"
     CDS             complement(4154..5080)
                     /gene="glsB"
                     /locus_tag="KW011_003406"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HBH7661662.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(5144..6532)
                     /gene="sad"
                     /locus_tag="KW011_003407"
     CDS             complement(5144..6532)
                     /gene="sad"
                     /locus_tag="KW011_003407"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HBH7661663.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            6633..7514
                     /locus_tag="KW011_003408"
     CDS             6633..7514
                     /locus_tag="KW011_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH7661664.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            7591..8706
                     /gene="yneK"
                     /locus_tag="KW011_003409"
     CDS             7591..8706
                     /gene="yneK"
                     /locus_tag="KW011_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HBH7661665.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMFNPGVPIKIAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLFLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLELFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            8856..10046
                     /gene="ydeA"
                     /locus_tag="KW011_003410"
     CDS             8856..10046
                     /gene="ydeA"
                     /locus_tag="KW011_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HBH7661666.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(10071..10736)
                     /gene="marC"
                     /locus_tag="KW011_003411"
     CDS             complement(10071..10736)
                     /gene="marC"
                     /locus_tag="KW011_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HBH7661667.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            10948..11382
                     /gene="marR"
                     /locus_tag="KW011_003412"
     CDS             10948..11382
                     /gene="marR"
                     /locus_tag="KW011_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HBH7661668.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            11402..11785
                     /gene="marA"
                     /locus_tag="KW011_003413"
     CDS             11402..11785
                     /gene="marA"
                     /locus_tag="KW011_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HBH7661669.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            11817..12035
                     /gene="marB"
                     /locus_tag="KW011_003414"
     CDS             11817..12035
                     /gene="marB"
                     /locus_tag="KW011_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HBH7661670.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(12092..13531)
                     /locus_tag="KW011_003415"
     CDS             complement(12092..13531)
                     /locus_tag="KW011_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBH7661671.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         3588 a         3419 c         3047 g         3516 t
ORIGIN      
        1 ggcggataaa tcgacagatt cagcaagatc attgcttaaa aaccgtggat taaaataata
       61 ctctgcgtca acgggaaata ccgcagttgc taagccaagg gcaatggtaa gccgtgcaaa
      121 gcgaggcagt attaacacct gatgcatacg atatgcctta aatgcggcac gaaaggctgt
      181 cattattaat tccgattgag tttattatta attaggtcac tggcttactg catggttgcc
      241 ttaatacgcg gagttaatgc accgtaatca ttgatcgtct gaaaactgat atccccggtt
      301 gcagcatgcg ggatatccac cgatacttca ccttttggcg gcaccatggt gtttgggaga
      361 ttgctatttc ctgctttcat atttgttacg gtaatgaaat agggcgttgg attaataaga
      421 gtgagtttgc taccgctacg acgaaaccga agcattgcag gcgcttcttc aggtgccatt
      481 gccaggttcg tggggcgata aaacattttt atgcggctaa taattgccag ctgtaatgta
      541 ttctcattct tctggtcttt ttccatagca ggaatagctt taacattcac ccagaacagg
      601 ctttcacggt cgcctggtaa ctgatgatta gtagcgttga ttatccgcag cgtattccag
      661 tgcgacagct gcgttggcaa cggtgctatg gcaagcaaag agcaataaaa gtaacactgt
      721 cgccataaag gaaatagagt agggggtacg cgtagtttgc atctgatgca ttctccatgt
      781 gttaagagga taagcgggaa aaccgttaca tcagacaatc gattaatgtt gctaatacat
      841 tggcgagtcg ccgggtttcc agacttaatg cgtttgtaat aaatgttggt tctcttttaa
      901 taggaaaacc ttgctacaaa gtaaatattt taaataacag taagtttaac tactgttgtt
      961 tattgatagt ttagatatta tgtgattttt catagcgctc caaatacaaa ccactcctca
     1021 ttaataatga gattcacatt ggtgaattgc tcgggtgtgc gtagccatgc cacacacacc
     1081 ctgtttcaat ttcagatgac gtatagccac cttaagattg aattatagat attgaacttt
     1141 gaaggtcgca tcagcgtttg cagtaccagc tacggactgc ccgagagcaa tataacgagc
     1201 ctggaatggc agtatattcg tgccacgata acatctttaa atcacaagga cacctaatct
     1261 gaacgaataa cgactaccag aaaagcactt cgcatcactc agacatgatt taacataata
     1321 tacattatgc gcacaaacat aaaccaaggg agaatccagt cgttggccgg tcatgattgc
     1381 tcattaacaa cgaccaaacc ccatatctca cttactaccg tattctcggc ttaaccgtcc
     1441 atgcaacctc aacacattgg tttcaactgc cgtcaccacg ttctccggaa aatctgtcgg
     1501 taatgaagtc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
     1561 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
     1621 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
     1681 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
     1741 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
     1801 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
     1861 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1921 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc gatcaactca cacgggatga
     1981 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     2041 tcagttttgc cggatagctt atcgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     2101 tgatccccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     2161 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     2221 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtatacagac
     2281 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     2341 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     2401 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     2461 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc tgtttgaatt
     2521 ggtacgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     2581 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     2641 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     2701 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     2761 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     2821 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     2881 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2941 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     3001 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     3061 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     3121 atgaccgtca gaggcacgat caacacgccc gttgagaatt gctcactgaa ccagtccgca
     3181 agcaaaggcc agaaatccag actatcaata ctgaccgaac caatcgcccc gacaatagcg
     3241 cataataatg cggcaatcag acagtaatta aatagccgta aggcacttac tggctcatac
     3301 ttatttttcc ccagacgctt atcccgcacg accagtaagg cgacggtaac aataaacatc
     3361 atattggaga aattgataac cagtgaaacc agtccccatt cggtggttat ggcatcataa
     3421 accagcatcg ccacataact gatcgcatag taatgcagtc gattaagcca gacatagcgg
     3481 gcaaaccccc ccgccattac gccattcagc ggccagaaaa gagaaagttc gtgaaccagc
     3541 cgtaactctg caccaataaa atagaacagc gtggttagcg caaaaatagc gatgctatta
     3601 cgtagcagat gaccttcctg gaacaaacgg aaagttgaaa tggggtgtac atgcattaat
     3661 tatctgtcca tacaattgcg ggtggcaatt atctcaacgt aataagtaaa ttctcataaa
     3721 ggatagcatg ttcgctaaca gcggcggcaa ataaaggtac cctgggaatg ctattttatc
     3781 gttttccgca attctcatgg ctgattcatc tgtacaacca gccagtgata gattaccgcc
     3841 acgatataac gttgttgttc ttcgcttaac gaaacaccct gcggaatgtt atgccactta
     3901 gccagacaac cgcgacagca ggtcgcggtg gcgtgctgag cgataaacac cggatgcccg
     3961 cgcatggggg tttgcttacc gtcattagcc ggtaatgccg gagcaagacg tttagcgaca
     4021 aaatccgccg catgttgttc gatgactggc gcgcctttct ccaggcaata ctgacgctct
     4081 ttcatgccca gacgaaagcg agagcgaaat tttgaacggg ataaacgcgc gaagagtgga
     4141 tcgagagact gcattaataa accgaacgcc ctaactgttt cgtcaattgt tcaaggacgg
     4201 cgatacccgc aagcgagtta cctgcatcat ccagttccgg actccagact gcgatggcca
     4261 tttcatgcgg aacaatcgcc acaataccgc caccaacgcc agatttcgcc ggtagtccca
     4321 cccgccaggc aaactccccc gcgttctggt acataccact ggtcgccatc agcgcgttaa
     4381 tttgccgcgc ctgcattggc gtcactacag gttcatcaat atgaatagct ttcccctgat
     4441 tagccagaaa gacaaacgtc cgagccagct ctacacagct cattttcaga gcgcagtaat
     4501 gaaagtagtt ttgcagaacg gttgtcacgt catgatggaa attgccaaac gacttcatca
     4561 gccaggcgat agccgcattt cgcgcggaat gttcaaattc ggaacgcgct accaccgtat
     4621 cgtatgaaat atcagacaca ccgcttaagc cgcgcacgac ttccagcata cgttgccgtg
     4681 gtgcgcttaa tcgcccttgc aacatatcgc agaccaccag cgcaccagca ttaataaacg
     4741 gattacgcgg aataccctgt tccatttcca gttgcactaa agaattgaac ggtgatccag
     4801 acggatcttt gccgacgcgt tgccagattt cctcttcgga gtaatgacgc atggcgacaa
     4861 cgagactcag cactttggaa atagactgaa tggaaaaacg ttcttgcgcg tctccggcct
     4921 gaaaaagctg tccgtcaacg gtacagatag caatgcccaa tcgggaaccg tctactgtag
     4981 ccagcgccgg aatataatcc gcgactttac cctgaccaat gagcggccgc acttgccgca
     5041 agatgttttc taaaattgca ttatccatgg cgactgccac tttctactcc tggaccgcag
     5101 gtctgaaaag acctgcgagt atatcagagc tgaatatgtc gcgtcagatc cggtctttcc
     5161 acaccgtctg gatattacag aattcgtgta agccgaaatg ggaaagctca cgaccaaagc
     5221 cactcttttt cacgccacca aaggccactc gcgcgtcgct ggcacaataa ccattgataa
     5281 acaccccacc gcattccaga cgtgccgcca tctgtctggc ctgtgtttcg tcggtggtaa
     5341 aaatggtcgc tgaaaggccg aactcactat cattagccag ttccagcgca tgttctgcat
     5401 ctttcgcaac ggtgattgcc gcaacggggc caaacatttc ttcccgaaac gcggtcattt
     5461 ctggggtaac attcgccaga accgttggcg gatagtagtt acctgcccca gccatctttt
     5521 ccccgcccag taacaaacgc gcaccctgcg ccagggtttt ctctacctga tgatgcagct
     5581 catcacgtaa atcaaaacga gccattggtc cgagagtgtt ctcttcgtca cggggatcgc
     5641 ccattttcaa ggctgccgca gctgccacaa aacgttcggt aaatgcagaa gcaattccct
     5701 cttcgataat aaagcgtttt gccgctgcac atacctgtcc ggtattctga taacgtccgg
     5761 ctaccgccgc tttaaccgcc agttccagat cggcatcgtt aagcacaata aacggatccg
     5821 aaccgcccag ttccagtacg cattttttca gtgccgctcc agcctgtgcg ccaatagccg
     5881 ctccagcacg aacacttccg gtcaccgtga cagcagcaat gcgcgagtct ttaatcatct
     5941 gactgacacc gtcgttgtcg gcattcagcc agccatatac gccttgcggg attcccgcat
     6001 ctttaaacac ctgggcaatg agctgagcac atcccatcac attcggcgca tgtttaagta
     6061 agtagccgtt acctgcaaga atgatgggaa cagcgccacg catcacctgc cataacggaa
     6121 aattccacgg cataatcgcc agaatcgtcc ccaacggtcg atactcaata accgcctgct
     6181 gattttccac cagtgtaggt tccgccttca gcattgccgg accatgttct gcataccagt
     6241 cacacaaatt cgccgatttc gccacttcag cgcgcgcctg gttgattggt ttgcccattt
     6301 cgcgggtgat catttgcgcc atttcttcgc tacgggcgcg cagagcctta ccgatatcac
     6361 gcattttttc agcacgataa tctatatttg tctcgcgcca gtcgcgaaag cctgctgccg
     6421 ccagctcaag tgcgttttcg atatcgttag cgccagccca cggcagcaca gaaagttgtt
     6481 cacccgtggc aggatttatt gaaattgcat gagttgccgg agtaatggtc atcggggtat
     6541 ctcctttatg agtcatggta tgaagatacg cagatttact cttgctttaa aatgaataat
     6601 attaagccac ttattcacga atcgagaatg ttatggatct gacccaactg gagatgttca
     6661 acgcggttgc cgaagctggc agcattaccc aggctgcagc aaaagtgcat cgcgtaccgt
     6721 cgaatctcac tacccgtcta cgccagctgg aaacagaact gggggtagat ctgtttattc
     6781 gcgagaatca gcgtttacgt ctctctcctg ccgggcataa ctttttacgc tatagccaac
     6841 aaattctcgc gttagtggat gaagcgcgga gcgttgtcgc tggcgatgag ccgcaaggtt
     6901 tattttctct tggttcgctg gaaagcaccg cagcagtgcg cattccggcc acgctggcgg
     6961 aattcaacca tcgttatccc aaaattcagt tttcactttc caccggccct tccggcacta
     7021 tgctggaagg agtgctggag ggaaaactga atgcggcatt tattgatggg cccattaacc
     7081 atactgccat tgacgggata ccggtatacc gcgaggagct gatgatcgtc acgccacaag
     7141 gtcatgcgcc agtaatccgc gccagtcagg ttaacggcag taacatttat gccttccgca
     7201 ccaattgttc gtatcgtcgc cacttcgaga gctggtttca tgctgacggt gccgctccgg
     7261 gaactattca cgagatggag tcgtatcacg ggatgttggc ctgtgtggtc gcaggagcag
     7321 gcattgcgct tattccacgc tctatgctgg aaagtatgcc ggggcatcac caggttgaag
     7381 cgtggccgtt agctgagcaa tggcgttggt taacaacctg gctggtctgg cgtcgcggtg
     7441 cgaaaacgcg tccgcttgag gcatttattc aactactgga tgtgtctgac tcggcaaaac
     7501 agagttacca atgaactatt ttgatagttc ttgcggctaa tatgctctat atagtgatgt
     7561 tccgatgact tatgactata tggggcaaat atggttacgc cagtaagcat cagcaattac
     7621 atatctcttc ctgatgattt tcccgttcgt aatattgcac cacaggtaaa agaagtttta
     7681 aaggacttta ttgatgcgct aagtacaata atatgtgatg aagagtggcg cacgtcttta
     7741 aacatcaact cagccacaaa aaagatattt aataaccttg ataacttatc atatattcag
     7801 aggacttctt tccggggtaa cgacacgcta tacaatgaaa aggttcagtt taaactcact
     7861 tatcccgtca agaatggaag acacaaagaa aatattgaat ttcaggtagt aataaattta
     7921 agccctattt atttagataa ttttcgccat gatggagaaa ttaatatttt ttgcgctccc
     7981 aacccaaagc ctgtcactat ggggcgcgta tttcagaccg gcgtcgagcg tgtgctcttt
     8041 ctttttctga atgattttat tgaacaattt ccaatgttca accctggcgt tccgatcaaa
     8101 atagcgcata caccacatat tgaacccctg cctccggatc accataccgc tgcagattac
     8161 ttacgccagt ttgatttgct tgtcctgaat tttatctctc gcggtaattt tgtcatactc
     8221 ccccgattat ggaataactc tgaggttcac agatggttcg tcaataaaga tcctaacttg
     8281 atcaccgcca ttctcgacat aacggacagc gaattaaaag aggatttatt gcaaagcctg
     8341 atggattcat taggttctaa caaacatgta ctacccgaag tctgcatctg ctttttattc
     8401 cttttagcag aacaagaatc tcctcatttt caggacttgt ttttattttt cgccaatatg
     8461 ttattgcact atcaccaatt tatgaatccc aatgaaagtg atttgaatga cgtgttgatg
     8521 ccagcatcat taagtgatga taaaattatc aagcatatgg cgcgcaggac cctcgaactg
     8581 tttgtaaaaa atgaaacacc gccaaaagtt actcacgaag acctggtgaa aaacaggcct
     8641 cgctcccctg tcagaccacc tatacccgca acctccaaaa cgccagacct ccctgaacgt
     8701 cattaaaccg tgatgttacc gactctctga cgcggaacag aatcagcgtc agagaaacgg
     8761 aaaacgcgat ccagatcaca aatacattgt attcacatca ttaaccgttt taagatcatt
     8821 tcatcacttt ttcgcaactc acccgataat ctgttatgac aacaaacact gtttcccgca
     8881 aagtggcgtg gctacgggtc gttacgctgg cagtcgccgc cttcatcttc aacaccaccg
     8941 aatttgtccc tgttggcctg ctctctgaca ttgcgcaaag ttttcacatg caaaccgctc
     9001 aggtcggcat catgttgacc atttacgcat gggtagtagc gctaatgtca ttgcctttta
     9061 tgttaatgac cagtcaggtt gaacggcgca aattactgat ctgcctgttt gtggtgttta
     9121 ttgccagcca cgtactgtcg tttttatcgt ggagctttac cgttctggtg atcagtcgca
     9181 ttggtgtggc ttttgcacat gcgattttct ggtcgattac ggcgtctctg gcgatccgta
     9241 tggctccggc cgggaagcgg gcacaggcct tgagtttaat tgccaccggt acagcactgg
     9301 cgatggtctt aggtttacct ctcgggcgca ttgtggggca gtatttcggt tggcgaatga
     9361 ccttcttcgc gattggtatt ggggcgctta tcaccctttt gtgcctgatt aagttacttc
     9421 ccttactgcc cagtgagcat tccggttcat tgaaaagcct cccgctattg ttccgccgcc
     9481 cggcattgat gagcatttat ttgttaactg tggtggttgt caccgcccat tacacggcat
     9541 acagctatat cgagcctttt gtgcaaaaca ttgcgggatt cagcgccaac tttgccacgg
     9601 cattactgtt attactcggt ggtgcgggca ttattggcag cgtgattttc ggtaaactgg
     9661 gtaatcagta tgcgtctgcg ttggtgagta cggcgattgc gctgttgctg gtgtgtctgg
     9721 cactgctgct acctgcggcg aacagtgaaa tacacctcgg ggtgctgagt attttctggg
     9781 ggatcgcgat gatgatcatc gggcttggta tgcaggttaa agtgctggcg ctggcaccag
     9841 atgctaccga cgtcgcgatg gcgctattct ccggcatatt taatattgga atcggggcgg
     9901 gtgcgttggt aggtaatcag gtgagtttgc actggtcaat gtcgatgatt ggttatgtgg
     9961 gcgcggtgcc tgcttttgcc gcgttaatat ggtcaatcat tatatttcgc cgctggccag
    10021 tgacactcga agaacagacg caatagttga aaggcccatt cgggcctttt ttaatggtac
    10081 gttttaatga tttccaggat gccgttaata ataaactgca cacccataca taccagcagg
    10141 aatcccatca gacgggagat cgcttcaatg ccacccttgc ccaccagccg cataattgcg
    10201 ccggagctgc gtaggcttcc ccacaaaata accgccacca ggaaaaagat cagcggcggc
    10261 gcaaccatca gtacccaatc agcgaaggtt gaactctgac gcactgtgga cgccgagcta
    10321 ataatcatcg ctatggttcc cggaccagcg gtacttggca ttgctagcgg cacaaaggca
    10381 atattggcac tgggttcatc ttccagctct tccgacttgc ttttcgcctc cggtgaatca
    10441 atcgctttct gttgcggaaa gagcatccga aaaccgataa acgcgacgat taagccgcct
    10501 gcaattcgca gaccgggaat cgaaatgcca aatgtatcca tcaccagttg cccggcgtaa
    10561 tacgccacca tcatgatggc aaatacgtac accgaggcca tcaacgactg acgattacgt
    10621 tcggcactgt tcatgttgcc tgccaggcca agaaatagcg cgacagttgt taatgggtta
    10681 gctaacggca gcaacaccac cagccccagg ccaattgctt taaacaaatc taacattggt
    10741 ggttgttatc ctgtgtatct gggttatcag cgaaaagtat aaggggtaaa caaggataaa
    10801 gtgtcactct ttagctaacc ttgcatcgca ttgaccaaaa cttgaaccga tttagcaaaa
    10861 cgtggcatcg gtcaattcat tcatttgact tatacttgcc tgggcaatat tatcctctgc
    10921 aactaattac ttgccagggc aactaatgtg aaaagtacca gcgatctgtt caatgaaatt
    10981 attccattgg gtcgcttaat ccatatggtt aatcagaaga aagatcgtct gcttaacgag
    11041 tatctgtctc cgctggatat taccgcggca cagtttaagg tgctctgctc tatccgctgc
    11101 gcggcgtgta ttactccggt tgaactgaaa aaagtgttgt cggtcgacct gggagcactg
    11161 acccgtatgc tggatcgcct ggtctgtaaa ggctgggttg aaaggttgcc gaacccgaat
    11221 gataagcgcg gcgtactggt aaaacttacc accagcggcg cggcaatatg cgaacaatgc
    11281 catcaattag ttggccagga cctgcatcaa gaattaacaa aaaacctgac ggcggacgaa
    11341 gttgcaacac ttgagcattt gcttaagaaa gtcctgccgt aaacaaaaaa gaggtatgac
    11401 gatgtccaga cgcaatactg acgctattac cattcatagc attttggact ggatcgagga
    11461 caacctggaa tcgccactgt cactggagaa agtgtcagag cgttcgggtt actccaaatg
    11521 gcacctgcaa cggatgttta aaaaagaaac cggtcattca ttaggccaat acatccgcag
    11581 ccgtaagatg acggaaatcg cgcaaaagct gaaggaaagt aacgagccga tactctatct
    11641 ggcagaacga tatggcttcg agtcgcaaca aactctgacc cgaaccttca aaaattactt
    11701 tgatgttccg ccgcataaat accggatgac caatatgcag ggcgaatcgc gctttttaca
    11761 tccattaaat cattacaata gctagttgat aacgtgacaa cgtcactgag gcaatcatga
    11821 aaccactttc atccgcaata gcagctgcgc ttattctctt ttccgcgcag ggcgttgcgg
    11881 aacaaaccac gcagccagtt gttacttctt gtgccaatgt cgtggttgtt cccccatcgc
    11941 aggaacaacc accgtttgat ttaaatcaca tgggtactgg cagtgataag tcggatgcgc
    12001 tcggcgtgcc ctattataat caacacgcta tgtagttatt gtcaggctct gatcacagag
    12061 cctgacatct ccgtaatatc ttcctcaagt ctcaaatatt ttcgccattg ttggcgatga
    12121 gatcccgata ccagtaaaac gacgctttac gtatccgttc cagcgttccc ttcccttcgt
    12181 tgtctttatc gacataaatc attccgtagc gctttttcat ttcccctgtt ccggcagaaa
    12241 ccaggtcaat gcagccccac ggggtgtagc caattaagtc aacaccatct tcaactacgg
    12301 cttttttcat ttcccgaata tgggaagcaa agtaatcaat gcgatagtga tcgttcaccg
    12361 tgccgtcagc ttgtctctgg tcaaccgcac caaatccatt ttcgacaata aacagcggca
    12421 actggaaatg atcccagaac cagttgagtg aataacgtag ccctgccgga tcaatttgcc
    12481 atccccattc ggatgtatcg atataagggt tgctgaccaa atctcgtggt tcaacataat
    12541 ccagttgcgg gttatcggga gaaaattgtg tcgtaaaaga catgtagtag ctaaaaccga
    12601 taaagtcgac acatccactg gcaagaatcg cgttatcttc tggtgtgata tcgaggttga
    12661 atcctttcct ggcaaagtaa ttcagcatat gttgcggata atatccacga gcatgaacat
    12721 cagtaaacca gtaacgacga tgcatcgctt tcgtggccat catcatatcg ttgggtgcac
    12781 acgtcagagg atagatgggg cacatggcga tcatacagcc aatgttaaat tcaggattga
    12841 tcgattttcc agtctgtacc gccagggcac tggcaactaa ctcgtaatgt accgcctggt
    12901 acattatttg ttcgcgctca ttgatatctt cctctggcga atacaagata ccggaattag
    12961 taaatggaca caggctttcg ctgaaattca cctgattatt gatttcgtta aacgtcatcc
    13021 agtactttac tttttcttta tagcgcgtga agaccgttga tgcgaagcgg ataaaaaagt
    13081 cgatcagctt acggtttcgc cagccaccat attttgtcac cagatgataa ggcatctcaa
    13141 aatgcgaaag cgtcaccaca ggttccattc cttgcttcag gcattcatcg aacagatcat
    13201 cataaaattg taaaccctct tcattcggct cctgttcgtc accttgcgga aagattcgtg
    13261 tccaggcaat ggaagttcga aagcatttga atcccatctc ggcaaataac tgaatatctg
    13321 ttttatagcg atgataaaaa tcaattgctt catgattggg ataattaagc ccgtcgataa
    13381 cgccttctgt cacttcacgc ggcaccccgt gagcgccagc agtcatcaca tcagcgatac
    13441 tgatgccttt tcctccttca ttccagccac cttccaactg atgcgcggct accgcgccac
    13501 cccataaaaa acctttttta aatcctgaca tacacaactc cttaactaaa atagattacc
    13561 accagacttc
//
LOCUS       DAESHI010000116        13549 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.117, whole genome shotgun sequence.
ACCESSION   DAESHI010000116 DAESHI010000000
VERSION     DAESHI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13549
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.117"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..312)
                     /locus_tag="KW011_003416"
     CDS             complement(<1..312)
                     /locus_tag="KW011_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBH7661672.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGSTTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LA"
     gene            complement(331..2208)
                     /gene="bglF"
                     /locus_tag="KW011_003417"
     CDS             complement(331..2208)
                     /gene="bglF"
                     /locus_tag="KW011_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HBH7661673.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="KW011_003418"
     CDS             complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="KW011_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HBH7661674.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="KW011_003419"
     CDS             complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="KW011_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBH7661675.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="KW011_003420"
     CDS             complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="KW011_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HBH7661676.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="KW011_003421"
     CDS             complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="KW011_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBH7661677.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="KW011_003422"
     CDS             complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="KW011_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HBH7661678.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="KW011_003423"
     CDS             complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="KW011_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBH7661679.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8292..9365)
                     /locus_tag="KW011_003424"
     CDS             complement(8292..9365)
                     /locus_tag="KW011_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBH7661680.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGGKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(9376..11898)
                     /locus_tag="KW011_003425"
     CDS             complement(9376..11898)
                     /locus_tag="KW011_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBH7661681.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSCVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            complement(11923..12654)
                     /locus_tag="KW011_003426"
     CDS             complement(11923..12654)
                     /locus_tag="KW011_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBH7661682.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            complement(12702..13274)
                     /locus_tag="KW011_003427"
     CDS             complement(12702..13274)
                     /locus_tag="KW011_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBH7661683.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSLVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT         3599 a         3419 c         3035 g         3496 t
ORIGIN      
        1 cgctaacccc gcttcattcg gttcggcttc gtcgccctga gggaaaattc gcgcccaggc
       61 aatggaaata cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta
      121 acggtgataa aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc
      181 catttttccc ataacgccat gaggctgtaa atctgaggtc gtgctccctt tgccatcttc
      241 cagccaggca ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg
      301 aaatgctttc ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc
      361 tgcgcttatc tgcgccgtgc cgtggggtaa tacgtctgta aaatcatcgc tattactgat
      421 taataccggc gtcgtcacat caaatccggc ctcgcgaata gcagggatat caaaagaaat
      481 cagccgatcg cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc
      541 gtccagtttt acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc
      601 aatgccaatg gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg
      661 cacttcacca accgagggca gaatggcgat acctttaccc aacaggccac tggcaaacgt
      721 ggtatcagcg acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc
      781 ctgctcaggt ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg
      841 acgtttagcg gtgatgaaat gaagcatcac cgtaccgaca aatgcgcaac cgatggcaat
      901 gacaccgcca ataacgctgg cccagacggt gaaatcaatt cccgttgacg ggatggtttg
      961 catgaaggtg aaaatacttg gcaaaccaaa ggagtacact ttcgtttgcg cgtagccaat
     1021 gatggtggcc cccaaagccc cactgataca ggcgataaca aaggggtact tacgcggcag
     1081 attaacgcca tataccgctg gttcggtgat accaaacagg cccgtcaacg ccgctgatcc
     1141 cgccaccact tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac
     1201 ctgcgccata atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa
     1261 gttattgata cacagcggca ccaggcccca gtgcagtccg aacatgacga agatttgcca
     1321 gaagccgccc attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc
     1381 ggcggcaatc agttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg
     1441 tgtgataacc atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca
     1501 cgcattaagt cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac
     1561 cgatgacgag taattcaaca atgtgaccgg aatacccagg aaatccagcc ccagcgcatc
     1621 cgctttttgc ccgttctcga aagcagtcag aattaatgga tgcactaacg ctccaccaat
     1681 caccatggca gtaaatggat taccgccgaa gcgtttcccc gcggtgtatc ccaggattat
     1741 cgggaagaac caaaacaagg catcactggc gctgaataaa attaaataag taccactttg
     1801 ttcggtcgtc cactgaaaag tgagcgccag agccagcata cctttcagga tcccggttgc
     1861 cgccatcaaa ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag
     1921 cagattacct ttctcatcat tttccggtgc ctgttgcgct ttttcgccaa ggcctgccac
     1981 gctgttgacc gccaggaaga catcggccac atggttacct atgaccacct gaaactggcc
     2041 accgctttcc accaccataa taataccggg ggtgtttttc agtacctcag cttgcgcttt
     2101 gctttcatcc tttaacttaa aacgtaatcg cgttgcgcaa tgcatcagac taacaatgtt
     2161 atctgcgccc ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct
     2221 accgctttgc ggcaaaactc ccaaaaaaaa actgaaaaaa acggcctgac gtgaatcaag
     2281 caattttttt caggttttgc ccgcttagtg cggtaacaat cctttactca gtaataatat
     2341 ttcagtgttc tttgcgcacg cgctctatat ttatggctaa aaacataatc tctgcgggtg
     2401 aaattttacg ttgatactgc aaaccaataa aaatggcgat ccgttccgca cattgccatg
     2461 cttgcgggta attttgtttt actgcttgtt gtaatgattc atcactatcg ttaattgaag
     2521 catgttcaag aatacgccag gataaaaact tcagatgtgt aaccagtcgc tgataactca
     2581 agctttcttc ctggtaatta aggctgaact gaaattttat taattgcagc atttcgcgca
     2641 ttaactgcgt gacacctgca acatcctcca tatttccgct catttgggca ctgaccagat
     2701 gcatggcaat aaagcccact tcatctttcg gtaactgcac gcccaaccgt ttatcaataa
     2761 tggttaacgc ttcttcccct agctggaact ctttcgggta aagccgctgg atatcccaca
     2821 gcaacgggtt gggcagcaac acgttttgct gaaagcgttt aatcgcaaac tggcaatggt
     2881 cagttagcga gatataaata ctgtcctgta attttcccaa gcgctcctgc gctaaagaga
     2941 taatacgatc acaggttgcc atcacctcaa gaggaatatg acttaagagt tcgcttaatc
     3001 gcccgttcag ttcatgactg ctcaaggcat actctttttc tattccactt gagttaattc
     3061 tttcgccagc gcatttttga aagccaattc ctcgccccat gacgactttt tcccgctgtt
     3121 gatcatcaat aaccaccaca acattattgt tgagaatttt ggtgatttgc atgttcatag
     3181 caaggacctt ttttataaac aaaaaaaacc gacttcacca gtattctctg gttatgtcag
     3241 gttttgcctg cgaatgcagt aacaatccag tcatttatta atggttttta taacgaacat
     3301 ccaggttcgg aaattaattt aattgcgtgc agagaaaata gcaatgcgct attgataaaa
     3361 atatgaccat gctcgcagtt attaactttg tgtaatttta ggaatttata aagttatata
     3421 taacaaatcc caataattaa gttattggga tttgtctggt gaattatttg tcgctatctt
     3481 ttcccgccag cagtttatcc agctcatcac cgcctacatg acggaaatcc tgccccttca
     3541 cgtagtagaa gataaactca caaatattct ggcagcggtc gccaatacgt tcgatagaac
     3601 gcgcgcagaa cagcgcagta agtacgctcg gaatggtacg cgaatcttcc atcatgtagg
     3661 tcatcagttg acgaacaata ccttcgtatt cctgatcgac ttttttatct tcacgataaa
     3721 tacgtaccgc ttcgtcaatg tccatccgcg cgaacgcgtc cagcacgtcg tgcagcatct
     3781 ggatggtatg acggcccagc gactccagac ttaccagcaa cggctgatgc tgctgggaga
     3841 atttctccag cgcagtacgg cagattttgt ccgccacgtc gccaatacgc tccagctcgg
     3901 caatggtttt actgatcacc ataaccagtc gcaggtcgct cgccgtcggc tgacgtttgg
     3961 cgataatgcg cacgcaggct tcatcgatcg ccacttccat catgttgacg ttcttgtcgc
     4021 cttcgatcac gcgcttcgcc agatcgctgt cctggttatg catcgcggtg attgcatcag
     4081 aaagctgctg ctccaccatg ccgcccatgg tcatcacctg cgtgcggata ctttccagtt
     4141 cggcgttgaa ctggccggaa atatgtttat taagattgag actgtccata acgcactcct
     4201 gaatcaaccg taacgaccgg tgatgtagtc ttctgtttgt ttcttcgctg gcttggtgaa
     4261 cagatcgtcc gtgttgctga actcaatcaa ttcgcccagg tacataaacg ccgtgtggtc
     4321 ggaacaacgc gcagcctgct gcatgttgtg ggtaacgatc accacggtgt aatcctgctt
     4381 cagttcggtg atcagctctt caatacgccc ggtagagatg gggtcgagcg ccgaacacgg
     4441 ttcgtcgagc agcagcactt ccgggcgaat ggcgatacca cgcgcaatac acagacgctg
     4501 ttgctgacca ccagagagag agtaaccgct ctggtgcaat ttatctttgg tttcgttcca
     4561 caatgcggct ttggtcaatg cccactgcac gcgctcgtcc atgtcggcac gggagagctt
     4621 ctcaaacaga cgaacaccaa aagcgatgtt gtcgtagatg gacatcggga acggcgtcgg
     4681 tttctggaac accatgccca ctttcgcacg cagcagtgcg atatcctgag agttggtcag
     4741 gatgttgtcg ccatcaagca gaatttcacc ttctgcgcgc tgctccggat acagttcaaa
     4801 cattttgttg aaggtacgca gcagcgtcga tttaccgcag ccggacggcc cgataaacgc
     4861 cgttacctgg tttttagcga tatccaggtt gatgtttttc agggcatgga atttgccgta
     4921 gtagaagttc aaattacgaa cctgaatttt actcggggca gtttcaacca tactcattgc
     4981 aatcttttcc tcatcgtacc tgatgctttt cgctaatcag gcttaccgta ggccgggtaa
     5041 ggcgttcacg ccgcatccgg caaagtgtta accgtgttta ttcttcgcaa aaacaacgcg
     5101 cgccagaatg ttcagcagca ggacgcacag ggtaatgatc aataccccgg cccaggccag
     5161 ttgttgccat tccgcaaacg ggctcatcgc aaacttaaag atcgtcaccg gcaggttggc
     5221 gatcggctgc atcatgtccg tgctccagaa ctggttggag agcgcggtaa acagcagcgg
     5281 cgcggtttca ccggcaatac gggcaatcgc cagcaggata ccggtcataa tcccggacac
     5341 cgacgctttc agcgtaatcg cggagatcat cttccacttc ggtgtaccca gcgcataagc
     5401 cgcttcacgc aggctgtccg gcaccagttt cagcatgttc tcggtggtgc ggataacgat
     5461 cggcacctgc aacagcgcca gcgcaattac gcccgcccag ccggagaagt gctccatctg
     5521 tgccaccacg atggtgtaga caaacagacc aaccacaatc gacggcgcgg agagcagaat
     5581 gtcgttaatg aagcgaatca cttctgccag ccaggattta cgaccatatt ccgccagata
     5641 aatccccgcc ataatgccca gcggcgtacc gaataccgtg gcccacaaaa ttaacagccc
     5701 gctacccgcc agggcgttcg ccagaccacc accttccgta ttgggcggcg gcgtcatttc
     5761 agtgaacagc gccagcgaca taccgtcgat accgcgagtg atggtggaca ttaaaatcca
     5821 gatcagccag aacaggccga aggccatcgt cgccatcgag agcgtcagcg caatacggtt
     5881 tttgaggcgg cgacgcgcct gcattttgcg gcgagattca gccagcgccg cagtggtttg
     5941 catttcaacc atagccatta gcgtgccccc tcattcttag ccaggcgcat aatcataaac
     6001 ttcgatgcgg cgaggacgat gaaggtaatc acaaacagga tcaggcccag ttccatcagt
     6061 gcggcaacgt gcagaccgga ttccgcttcc gcaaattcgt tcgccaacgc agaggtgata
     6121 ctattgcccg gcatgtacag cgaggcgctg tcgagctggt aggtgttacc gatgataaag
     6181 gtcaccgcca tggtttcacc gagcgcgcgg cccagcccca gcatgatgcc gccgataaca
     6241 ccatttttgg tgaacggaag aacgatacgc cagataactt cccaggtggt gcagccaata
     6301 ccgtaggccg actctttcat catcaccggg gtttgttcga acacatcacg cattaccgcc
     6361 gcaatgtacg gaataatcat gatggcgagg atcacgcctg ccgcgaggat accgatacca
     6421 aatgcggggc cagagaacag cgcgccaaca atcgggatat tcgacatgat attgccgacc
     6481 ggctcctgaa agtaaacggc gaacagcggc gcaaagataa acaggcccca catgccgtaa
     6541 acgatacttg gaatggctgc cagcagctca atggcgatac ccagcgggcg tttcagccag
     6601 ccaggcgcaa gctcagtcag gaacagggcg ataccgaaac tcaccgggac ggcgatcagc
     6661 agcgcgataa acgaagtcac caacgtaccg tagatcggca ccagcgcccc gtagatatcg
     6721 ttcggtgcat cccactcttt ggtccatagg aaagccagac caaatttctg aatgctcggc
     6781 caggaggaga tgatcagaga gacaataatg ccacccaaca tcaatagcac aatcagcgcc
     6841 gccagtttta ccagcacgct gaaaattatg tcgccctttt tacccggtgg gttaaaagca
     6901 ggcttggttg cagccataag ttactcttca gttaaacgcg tttacaaagt cattttgtaa
     6961 tgccggatgc ggcgtaaaac accgtacccg gcctagagtt ttattagtac agcggcttac
     7021 cgctactgtc tttaatattg gtcttccacg cagcgcgaac ctgttcaact acactatccg
     7081 gcaggctggc gtaatccagg tcgttcgcct gtttagcccc ggttttgtac gcccagtcga
     7141 agaatttcag cacttctgtg ccttgttctg gtttcttctg atctttgtgg atcagaatga
     7201 acgtggtaga ggtaataggc catgcatctt cgcctttctg gttggtcaga tcctgagcga
     7261 aggttttgct ccagtctgca ccttttgctg cattagcgaa gttttcttcg gtcggactaa
     7321 ccggtttacc atcagcggag atcagtttgg tgtacgccag gttgttctgc ttcgcgtaag
     7381 catattcaac ataaccaatt gcacccggca gacgctgaac gaacgcggcg ataccgtcgt
     7441 tacctttacc gcccagaccg atcggccatt ttacggtaga gccagtacca acgttgtttt
     7501 tccactcttc gttcactttc gccaggtagc tggtgaagac gaaggaagtc ccggagccat
     7561 ctgcgcggcg tactacagca atgttttgtg aaggcagttt cagacccgga ttcagtttgg
     7621 cgatggtttc atcatcccac ttcttgattt tgcccaggta gatgtcgccg agggttttac
     7681 catccagcac cagttcgcca gacttcagcc ctggaatgtt aaccgccagc accacgccgc
     7741 caatcacggt cgggaactgg aacagacctt cctgagccag tttttcgtca gacagcggcg
     7801 cgtcagaggc accaaaatca acggtattag cgataatctg ttttacgcca ccggaagaac
     7861 cgataccctg gtagttaact ttattaccga tttctttctg gtaagtgtca gcccatttgg
     7921 catacaccgg cgcagggaag gttgcacctg cacctgtcag gcttgcttct gcaaacacag
     7981 agaaagcgct catcgataag gtcgcggcga caacagttgc gacggtggta cgcataactt
     8041 tcataatgtc tcctgggagg attcataaag cattgtttgt tggctacgag aagcaaaata
     8101 ggacaaacag gtgacagtta tatgtaagga atatgacatt tttatgacag aaaggaatta
     8161 acaaataact tactgatttt aaatgtgaag atgaaaatcc cacatgtcat ctttttgtca
     8221 tgatgcattt aaaagtgata aagaaatggg tgagatgaca acgatgtgat gaacaatcgt
     8281 tgtcagacct attagcgcac aatctcaaca gttatcgtcg ctgttgcagt aaattgcccc
     8341 ggggctgggc gagctccagt aaggctcgca ggagcagctg aaaaagttac actacctgat
     8401 ttttcaccat acactgttga tttacccatg tctgttggca actcaccacc gttcactttg
     8461 atttccttat tatttttgtc gtaaattttt atccccacgt tagagtttga agttacgacc
     8521 attgaattgt ccgtattggg ttcggtagca taaaaagacg tactgatatc atcggtattc
     8581 agtatcccca ggttttcaca ctcaatagtg actgttttag taattttatg tgttgttacc
     8641 gcacttccaa caacagatga aaaatctgca gctggtattt ctttaaaatc aaaataaact
     8701 ggctgtaaat tatttatttc gcaactttgc ggagctgtaa tatcgccccg aatataaaca
     8761 tcagccatcg gctgtgagta atccaccggg gtgtcacggc taattgttcc atacaatgtc
     8821 actatctttg tttcaggaat aatcaatttt cctgcaaatg tcttcttgat ataaaaatat
     8881 actatcgctt tacccccact ggatatactt aaaggttcaa tgcggttgca attgtacttt
     8941 gtattaccag acgggttgtt agcatgatat tcaaaaggtg cattcacata cccaacccca
     9001 agaatatcta tagcaatccc aacatcaagg ttcggattaa gatcataata attaatatta
     9061 gagtgaaccc cagcataagt taatgcctgg tttatttttg acgtataata gatatagggg
     9121 aattctccta cattaccagt atcacaatga cagcgtccag gatatgagga tccattagtt
     9181 acagtggtat taaatgtcgt tccaacctta ttttcgttcg cggtaataag atatcccgag
     9241 aaatcaagcg tacggtgaaa tacgccatta tcaggggtac agataccttt aggagctgca
     9301 agtgctacac tacttaacga gagcataaac acagcaaagc accactgttt gatatacttg
     9361 ttcataaaaa tccttttaat gacatgtcac accctgttca attaatccgc tctcactttc
     9421 tggtgtcgaa agtgaataat tgaagatgca ttttgttgag gcgttttctc cccaggataa
     9481 cgtaacctgt cctttttctg gtagaccaga aagataaagt tcgccacggt caccgacaat
     9541 tcctgaatct gccaaacttg catcttgtat cgcagcaatg gctccaaaag gtaccggttt
     9601 accatttgca tttaacactc tgaagagaac gcgataaccc acatgagtca caaactccgc
     9661 tttaaccact gcaccacgcg tgggaaccat cgtaacgacg gtattatcaa gttctatatt
     9721 cgacttcgag aaatagttat cgctgattgc tacccgattt tcatcataag ggtttagctg
     9781 tgtcttgacc gtatagccgc gccagtccgt cgcagcaccg cgcatattat ctatttctaa
     9841 cccggcggcc ccaggtgctt taacaagagc aatagtttca cctaactcct gacttaacgt
     9901 taccccttcg ctgtgtgcca gaattccccc tctgatacca taattcagcc tctgggaatt
     9961 ttggctgtag ttatagctgc cgttcaccgt gccataagag ccatcatagg tcagggacat
    10021 attaccgcca acctcatcat catgactgtt ataggactgc attaaattcc atgaaaggtt
    10081 atttctttcc agcgccgtac cgctgagtcc gagttgatgt gagctaccgg aattatcgtt
    10141 ttgcgaaaca gaataggtgg cgtaactatt accccaccag cccaataaag gaacagataa
    10201 agagagcatg tagatctgct cactggtgtt atcactggca tggttgttcc ccgtcccctg
    10261 tctggcagta ttggaataac tccaggagac gttccacgag agacgcttcc agctactgct
    10321 gtagccaaat tgcattaatc gttcgacatc atcggtatgc cagtattgtt gccggcttaa
    10381 tgtcaggtat aaattgccat aatctctccc caacgactgc gttagcgtcc cctccacgca
    10441 actacgtcgg ttacctgttt cccaaaagtc ttcagggctg ttgcgccgcg atgcccactc
    10501 attgagggta tagtagccct cggtggagta acgatagcca accaagcgca agctagtgtc
    10561 tgtgtcgtca aatcgttttg aatagagaaa gcgatatgac tgacccgttt ctgtatcatc
    10621 atggctaaaa ttcgcacgag catgtgtcac atcgaaagaa attgcgccca aagcgccaag
    10681 atctttaccg acacccagag ctgcagaacc ataattttca gcagcctgca tacctccata
    10741 taaagtgatc ccgtgggaaa aaccatgaag tatttgcgcc tgaaggacgt ccggtgtaaa
    10801 tccgtcttca tcgcgcaatt cacccacgct gacatcaaca tctgtctgac cttcacgttt
    10861 aagaatcgct aatgaagcat aaggttgggt gaatcgttgt tcgctaccgt cttcttcttt
    10921 gatggttacg tccaaatcgc cacctgtact ggcggtgttg agatcggtga tctcaaaagc
    10981 accttgtgga acggcagact gataaatcac gtagccattc tgccgaatga tgacggtggc
    11041 gttggacttt gcaatcccac gaaccacagg agcaaagcca ttctggctgg cgggtagcat
    11101 atcgttatca gtatacaaac gcgcgccgcg aatttgcgta ctgtcgaaaa tatcgctcgc
    11161 cgtccacgta tcaccaatca taatctggct tcttaacgcg gcaatatcgc gctgtatcca
    11221 ggtactaatg gagttgtatt ccgcacggcc atcgctggtg tttaaagtac tgtaattacg
    11281 tagccgccac ggtccaatat tcagtccatt gcgcagattg agataactgg tatctgtctc
    11341 ttgtgaacca taatctgaac gtagtgtatg tgaaccagaa aaatcgtagt tgatggtgag
    11401 cgcgttaatc ccctcatccc aacgcgtggg gctgatgtaa tcataagcca tcgcgtccat
    11461 gtagatttgg ggaatggata tattcagctg caatttatca ctgtcaaact gccaggatga
    11521 gccttcaata cgtggagcag gatcgaggca aaccgtctca tccacatcat tgaacgattt
    11581 gagcgcatca acttttatgc caaaggagga cagtgttttt ttaatggcaa taaagcaagc
    11641 gtgcagatca gcatctggat tatcctgaac aaatgtcaca tcctgacgtt caaacatttt
    11701 accgttgata aaaatatcaa cgctataaac acctgggggc atcaggtttg cccgattaaa
    11761 ctggcgaata tcgactccgg attgatggtc gatttccaga agcgccggat tgaatacact
    11821 gacctcggcc atcaggtttt gcgagaaaat cagacacgtc cctgccgtga ggccaaccac
    11881 tattctggtc gtcatcataa tacgcctatc cttatccctt gcttacagcg tcatttttat
    11941 cgggtcactg actgcgccgt aatcattaat aaatcgccag gtcatttggc ctcccgcaga
    12001 attagcaggt aaaggtaacg tgagcgtttc aaaaggacgg atcatattga ggggcacatc
    12061 atggccatca acgatgacat ccatcacact gatatgaatt gggttgggat tctctgctat
    12121 cagatttcgc ccttcccggc gccatttcac tttcggcgca gcatcttcgg gaataaggcg
    12181 gatatttgct ggtcgccaga acagcttgat acgcgttttt actgctattt ccagcgagtt
    12241 cgtcgctttg ttgctcgctg atggcggaat atttttaata ttcagccaga agactgactc
    12301 gcgatcatca ggcaatgaca cacctttggt gtgaataatg cgtaatattt tttcttgcga
    12361 aggttctaat cgtgatactg gcggaataac ggtaaacggc gctttgcttt tatcctcttt
    12421 attaaatgga tctacccaag cctggatgag ataaggtatt ttttcttctt tgctaaatac
    12481 agaaatagat ttatcagcat tattacttaa gtaaaccacc cgcgtaccac caatgacaac
    12541 gcctgcataa gttaatgctg gttggcctaa aaaaacgctg agtataatcg cggtaaataa
    12601 cttacatgaa actgttgttt taaagagcct gattaaacaa ctttttctgg acataacgac
    12661 tccctggcca aagccaactg aataaaaagg cccttgataa attatttctt atattcgaca
    12721 gtaaattgtg aatcagcgtt cgccgtacca gccgttacgg tcgcattggt tgccacatag
    12781 cgtgcaataa acttcatttc agccttgcct tcagcatcga tagcggtata agcagagtcg
    12841 ttataaagtt taaccagcga attatcggac tggttaaaaa tacgaatacc aacgccggtt
    12901 gccgcaatcg cttgtcctgt accggtaaaa tcaccagggt tgcctgctgt aagcggcgta
    12961 cccactttca ggtcgccgtc gccgccaggc gcttctgtac catcaaaacg aacggctgct
    13021 tgagtgtacg ttgccgggca attttcaagt ttaatactga acttctgtgg cgacgccaga
    13081 ctacctacac ctgaaaatgc atttttactg actttaccca gtgggacaat ttgatcggct
    13141 gtatcggagg taacttcaca agtagtgtcg acaatttcac cgacgaagtg aacaacacca
    13201 tcttctgcca gtgcatgccc ggccagcatt agagatgcca gagtgaatgc accgcctata
    13261 atattacgct tcataaaaac tccattgata taatatatcc tttaccatga atcatcactt
    13321 atattaataa gtgtattttt gattcaaagg aatagtacac caaagagacg gctttatcta
    13381 aaaataatat taattacgca aaaataaaat ataaatttat tgtttaaata ttatttaaac
    13441 agtaaaaaat caagtttgtt atcagacatt aatgataatt cagaatatat ttatatagaa
    13501 agatagatga atttattaat gtcagttata aaaaagggcc accggtcgg
//
LOCUS       DAESHI010000117        13540 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.118, whole genome shotgun sequence.
ACCESSION   DAESHI010000117 DAESHI010000000
VERSION     DAESHI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13540
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.118"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..1137
                     /locus_tag="KW011_003428"
     CDS             <1..1137
                     /locus_tag="KW011_003428"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBH7661684.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     PKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGG
                     FLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYL
                     GGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYE
                     FICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMR
                     IMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            1507..2331
                     /gene="gadX"
                     /locus_tag="KW011_003429"
     CDS             1507..2331
                     /gene="gadX"
                     /locus_tag="KW011_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HBH7661685.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            2699..3427
                     /gene="gadW"
                     /locus_tag="KW011_003430"
     CDS             2699..3427
                     /gene="gadW"
                     /locus_tag="KW011_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HBH7661686.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(3790..6903)
                     /locus_tag="KW011_003431"
     CDS             complement(3790..6903)
                     /locus_tag="KW011_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HBH7661687.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(6928..8085)
                     /gene="mdtE"
                     /locus_tag="KW011_003432"
     CDS             complement(6928..8085)
                     /gene="mdtE"
                     /locus_tag="KW011_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HBH7661688.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            8145..8423
                     /locus_tag="KW011_003433"
     CDS             8145..8423
                     /locus_tag="KW011_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661689.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(8424..8951)
                     /gene="gadE"
                     /locus_tag="KW011_003434"
     CDS             complement(8424..8951)
                     /gene="gadE"
                     /locus_tag="KW011_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HBH7661690.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(9750..10322)
                     /gene="hdeD"
                     /locus_tag="KW011_003435"
     CDS             complement(9750..10322)
                     /gene="hdeD"
                     /locus_tag="KW011_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HBH7661691.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            10577..10909
                     /gene="hdeA"
                     /locus_tag="KW011_003436"
     CDS             10577..10909
                     /gene="hdeA"
                     /locus_tag="KW011_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HBH7661692.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            11025..11351
                     /gene="hdeB"
                     /locus_tag="KW011_003437"
     CDS             11025..11351
                     /gene="hdeB"
                     /locus_tag="KW011_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HBH7661693.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            11415..12062
                     /locus_tag="KW011_003438"
     CDS             11415..12062
                     /locus_tag="KW011_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBH7661694.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(12104..12634)
                     /locus_tag="KW011_003439"
     CDS             complement(12104..12634)
                     /locus_tag="KW011_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH7661695.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(12790..13356)
                     /gene="slp"
                     /locus_tag="KW011_003440"
     CDS             complement(12790..13356)
                     /gene="slp"
                     /locus_tag="KW011_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HBH7661696.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3673 a         3069 c         3128 g         3670 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggacccga aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgccgact
      361 ttcggcgtga cctacaccgg taactatgag ttcccacaac cgctgcacga tgcgctggat
      421 aaattccagg ccgacaccgg tatcgacatc gacatgcaca tcgacgctgc cagcggtggc
      481 ttcctggcac cgttcgtcgc cccggatatc gtctgggact tccgcctgcc gcgtgtgaaa
      541 tcgatcagtg cttcaggcca taaattcggt ctggctccgc tgggctgcgg ctgggttatc
      601 tggcgtgacg aagaagcgct gccgcaggaa ctggtgttca acgttgacta cctgggtggt
      661 caaattggta cttttgccat caacttctcc cgcccggcgg gtcaggtaat tgcacagtac
      721 tatgaattcc tgcgcctcgg tcgtgaaggc tataccaaag tacagaacgc ctcttaccag
      781 gttgccgctt atctggcgga tgaaatcgcc aaactggggc cgtatgagtt catctgtacg
      841 ggtcgcccgg acgaaggcat cccggcggtt tgcttcaaac tgaaagatgg tgaagatccg
      901 ggatacaccc tgtacgacct ctctgaacgt ctgcgtctgc gcggctggca ggttccggcc
      961 ttcactctcg gcggtgaagc caccgacatc gtggtgatgc gcattatgtg tcgtcgcggc
     1021 ttcgaaatgg actttgctga actgttgctg gaagactaca aagcctccct gaaatatctc
     1081 agcgatcacc cgaaactgca gggtattgcc cagcagaaca gctttaaaca cacctgataa
     1141 cataacgttg taaaaaccga atgcccagcc tttaaaaaaa cagctgggca ttcggttgct
     1201 tattaatcgc aataatatat tggctgtcta ttcatcgtgt tgataagata tatgttatat
     1261 gaatgtttat taaatgtagc gattgccttt aatgtcacga aaagggatta tttgcttgct
     1321 attaatttca ctgcgttctt aaacacattt ttgtattata aatccagtca tcctgcccgt
     1381 agaacatcac acattatcat cctgttctcc cgcttcgttt aaatttattt atcaatcaat
     1441 ttgacttaag agggcggcgt gctacattaa ttaacagtaa tatgtttatg taatattaag
     1501 tcaactatgc aatcattaca tgggaattgt ctaattgcgt acgcaagaca taaatatatt
     1561 ctcaccatgg ttaatggtga atatcgctat tttaatggcg gtgacctggt ttttgcggat
     1621 gcaagccaaa ttcgagtaga taagtgtgtt gaaaattttg tattagtatc aagggatacg
     1681 ctttcattat ttctgccgat gctcaaggag gaggcattaa atcttcatgc acataaaaaa
     1741 atttcttcat tactcgttca tcactgtagc agagatattc ctgtttttca ggaagttgcg
     1801 caactatcgc agaataagaa tcttcgctat gcagaaatgc tacgtaaaag agcattaatc
     1861 tttgcgttgt tatctgtttt tcttgaggat gagcacttta taccgctgct tttgaacgtt
     1921 ttgcaaccga acatgcgaac acgagtttgt acggttatca ataataatat cgcccatgag
     1981 tggacactag cccgaatcgc cagcgagctg ttgatgagtc caagcctgtt aaagaaaaaa
     2041 ttgcgcgaag aagagacatc atattcacag ttgcttactg agtgtagaat gcaacgtgct
     2101 ttgcaactta ttgttataca tggcttttca attaagcgag tcgcagtgtc ctgtggatat
     2161 cacagcgtgt cgtatttcat ttacgtcttt cgaaattatt atgggatgac gcccacagag
     2221 tatcaggagc gatcggcgca gggattgccg aaccgtgact cggcggcaag tattgttgcg
     2281 caaggaaatt tttacggcac tgaccgttct gcggaaggaa taagattata gaattttact
     2341 cagacataaa aaaaacccgg cataggggac cgggaagagg atagtctgcc gtctccagac
     2401 taataaaccg ttataacact ccctgttggc acgggaaact ttgtgctctc agtaagttaa
     2461 atataacttt tactggaaat aagatcagcc atttttttat aaacataagc tatacgctgt
     2521 gcgaaaatat attcgtgctg catttactta ttatcaatta actgttatgc aaaactactt
     2581 tgtggataaa ttttggtcct accaaatctg gcagtttttt cgctaagaaa cagtctggca
     2641 acatttcatt agtatactga aattgaaata atcgcagtat gaaatataag ggataatcat
     2701 gactcatgtc tgctcggtga tcctcattcg tcgttcattc gatatttatc atgaacagca
     2761 taaaatatcg ctgcataacg agagtatcgt gctgctggag aaaaatttgg cagacgattt
     2821 tgcgttttgt tcaccggata cgcgacgact ggatatcgat gagctgacag tttgccatta
     2881 cttacaaaat attcgtcagc taccacgcaa tttagggtta catagcaaag accgtctatt
     2941 aattaaccag tcacccccca tgccgctggt gacggcgatt tttgatagct tcaatgaatc
     3001 cggggtaaat tcaccgatac tgagcaatat gctctacctt tcctgtttat cgatgttttc
     3061 tcataagaaa gaactgatcc ccttactttt caatagcatc agcactgttt caggaaaagt
     3121 tgaacgcctt attagctttg atatcgccaa acgttggtat ctgcgcgata tcgcggaaag
     3181 aatgtatacc agcgagagtc tcataaaaaa aaagttgcag gatgaaaata cctgtttcag
     3241 taaaatatta ctcgcttcca ggatgtcgat ggccagacga ttactcgagt tacgtcaaat
     3301 tcctctgcat actattgcag aaaaatgtgg ctatagcagt acgtcgtact ttataaacac
     3361 atttcgacaa tattatggtg taacgccaca tcagtttgcg caacattcgc caggtacctt
     3421 ttcctgacat attttgcatt tgaatattgg tcaggatctc acacctgctt catgtgaaac
     3481 tcttccctga tgatttctgc cgggctaccg gctagttctc tttcgcagtt tgccaggcgg
     3541 tataggtatt ttcaggagga aaagactcgg catgtttggg attattaagc tgacaattca
     3601 tacgattacg ggaatgtggg tcagtattgt gctgttcaaa ttgatgacga atggctggag
     3661 tggtttctac tttcagtgct gtgtactaag cctggtattc ctgacggtta gctggttgtt
     3721 gagtggtgaa tggttagcag gaaagagtaa ggctgaacct tcacgttcaa ccttactctc
     3781 atttacacgt tacgcttttt taaagcgggc aaagagatgt tccactacaa cgaaaaagac
     3841 cggaacgaag taaattgcca gcactgttgc ggcaaacatc ccgcccatca cgccggtacc
     3901 taccgcgttt tgcgcgccag aaccggcacc atgactgata accagcggca gcacgccgag
     3961 aataaaggcg agagaggtca tcaggattgg gcgtaaacgc atccgcgccg cttcgatgat
     4021 tgcctctatc ggcgttttcc cttctttctg catcatctca acggcaaatt cgacaatcag
     4081 gatggcgttc ttggcggaaa gcccgatggt ggtcagcaaa ccaacctgga agtagacgtc
     4141 attacttaag ccgcgcagat cggtggccag taatgcgcca acgacgccta acggaacaac
     4201 caacatcacc gagaacggaa ttgaccagct ctcatagagt gcggcgaggg cgaggaacac
     4261 cacgaccagt gaaatggcat acagtgcagg agcctggttt gaggataacg cttcctgata
     4321 cgacagtccg gtccatgagt agccgacgcc tgccggaagt ttagcgacca ggtctgccat
     4381 aaatttcata gcgtcaccgg tacttttccc ggccgccgct tcacctaaaa tctccattga
     4441 cgggatgccg ttgtagcgtt ccaatcgcgg tgaaccatag gtccattcgg tagacgagta
     4501 ggcagaaagc ggtgccatcg tgccagaggc gttgcgtaca taccattggt tgatgttatc
     4561 cggcaacata cggaacggcg tgcctgcctg gacatacact tttttcaccc gcccctggtt
     4621 gaggaagtcg ttcacgtagc tgctgccgaa ggcggtggaa attgtctggt tgatatcaga
     4681 cagcgccaca cccattgcct cagctttcgc ggcgttgacg ttcactttga acatcggcgt
     4741 atcttccagg ccgttcgggc gtaccccggt gacctggtca ggcgattgcg ctgccagtga
     4801 taacagctcg tttcgtgcct gggttagttt ttcgtgcccc aggttaccgt tgtccagcag
     4861 ttccatatca aaacctgacg cggtacccag ttcagccacc gcgggtaagt tgaacgggaa
     4921 gacgacggct ttattgatac tgcttaacgc aatcattgcc cgctgaatga tcgcggtaac
     4981 cgagttttcc tcaccgacac gttcagacca cggcttgaga ctgataaacg ccaggccgtt
     5041 gttttgcccc tggccgctga agccaaagcc gccaacggta aacaccgact gaacattatc
     5101 tttctctttt gtaagataat aatccgtcac ctgttgcagc actttcgtgg tgttaaccat
     5161 ggtggcaccg gaaggtaact gcgcggtggt cataaatacc ccctgatcct cttctggtaa
     5221 gaaagaggtc ggtgtacgca ggaacagcac cgccatcccg gcgcaaatca gcaggtagac
     5281 caccatgtag cgaccggtac aacgcaacag cgagcgggtg ctatcggtat agtgctgagt
     5341 tgatttttca aacagcgtgt tgaagcgtgc gaacagggcg ttaggtttgt gaccgccttc
     5401 cggcgcggct ttcagaatgg tggcgcacag ggcaggggtc aggctcattg ccacaaatac
     5461 tgaaagcagc atggaggaga tcagcgtgat ggagaactgg cggtagatct ccccggttgc
     5521 gccgctcata aaggccatcg gcataaacac tgcggaaaga acaacagcaa taccgaccag
     5581 cgcacgttgg atctgcccca tcgatttatg cgtcgcttcc ttcggtggta gcttatcttc
     5641 cgcaatgaca cgctcgacgt tctccaccac cacgatggcg tcatccacca gtaaccctat
     5701 cgccagcacc atcccgaaca tagttaacgt gttgatggtg aaaccgaccg ccgacaagat
     5761 cgcaaacgtc ccgagaataa ccaccggtac ggcaatcgtc gggatgattg tggcacggaa
     5821 attctgcaaa aacagataca tgaccaggaa gacgaggatg atagcctcaa ccagtgtttt
     5881 aaaaacttcc tgaatagaaa tttcgataaa cggcgtggtg tcgtaaggat aaaccgtctt
     5941 cagacttgcg gggaaatagg ctgataagcg gttcagctcc tctttgactg cccgcgaggt
     6001 atccagggcg tttgctccgg tagccagttt gatggcgatc ccggcagcag gtttgccgtt
     6061 atagcgcgcc acggtggaat aatcttccgc tccaagttcg acgcgggcga catcacgcag
     6121 cagcacttgc gaaccatctt gctgaacttt caacaggatt ttgccaaatt cttccggcgt
     6181 ttgcagacgc gtctgcacaa tgatcgaggc gtttagttgc tggtctgccg cctgtggcat
     6241 gccacccagt tgaccaccgg aaatctggtt gttttgcacc ttaatctggg aaataacatc
     6301 ggaaggtacc agattgtatt tattgagttt ttgcgggtcc agccagatac gcatggcgta
     6361 ctcggaacca aagagctgta cgctaccaac gcccgcggta cggcttagcg ggtctttgat
     6421 attagacgct acatagtccg cgatatcgta ctggttgagg ctgccgttat cagaaataaa
     6481 cgccgctacc atcagaatat tactgctcga cttatcaacg ctaatcccct gctgctgcac
     6541 cgcttcaggt aatgaaggca tagcgagttg cagtttattt tgcacctgaa cctgtgcgat
     6601 atcaggagat gtcccagtct cgaaggtcag agtgatagag gcattacccg ccgcatcact
     6661 ggttgaagac atgtacatca ggccatcaag cccattcata ttttgctcaa tcacctgagt
     6721 gaccgagtct tctaccgttt gcgcatcggc acctggatag gtagcgctga cggtaatggt
     6781 cggtggcgca atctgcggat actgcgcaac cggtaagttc atgatcgcca gaccacctgc
     6841 aagcatcata ataatggcaa gtacccaggc aaaaaccggg cgatcaataa aatagttagc
     6901 catgaaagtc cccttaagcc tgcaacgtta ttgtttcgat tcggtgctgg cgttttcctg
     6961 gctggaggaa attgctcgtg ctttgatacc cggacgaatg cgttgcaaac cggaaacgat
     7021 cacccgatcg ccagcctgca agccagaggt gacaacccac tggtcgccga tggctttgct
     7081 ggcttcaatt tcgcgtagct gcacaacatc gtctttatcc agaatgagcg ccgttgcttt
     7141 accctgggcg ttgtgagtga cgccttcctg cggcaccagt aatacattct ggcggctacc
     7201 ttcatccact aatgccgtga cgtacatgcc aggcagcaag tcaccatttg ggttggggaa
     7261 aatcgcccgt aacgtcacgg agcccgtggt ttcatccacc gtcgggtcgg agaatttcag
     7321 tgtgccggtc tggctgtagc gtttaccatt ttccagattg agctgtactg gcgtactgcc
     7381 ctgaacctgt ttgatttgcc cactggcgac ctcttctttc atgcgcaaga aatcctgcac
     7441 cgactgcgtg agatcgacat aaatcgggtc cagacgttgt acggtaacca gcgaatctgc
     7501 ctgattagcg gtaacgagtg cgccgacggt caccgacgat ttcccgctga cgcccgtaat
     7561 cggcgaggtg acattcgcgt attgcagatt gatcgtcgcc tgttcaacag ccgctttggc
     7621 gacggtgaca ttggcttctg cttcattcag ctgggtgcgc gcggtgtcgt aatcctgacg
     7681 gctaacgtag ttggtcttca gcaacgatgc ctggcggtta aaggtgatgc gggcattgct
     7741 ggcggtagag agcgctttcg ccagcgagcc tttggcggag tttagctcgg cctgtaaagg
     7801 tgcaggatca atctgataca gcgaatcgcc ctggttcact ttatcgcctt cgataaagtt
     7861 gcgtttaatg ataataccgc ccacctgggg acgtatctcg gcaacttcat aaggaacggt
     7921 tctaccgggc aattcgctca acacattgac cgaaccgggg gagagtgtga cgacaccgac
     7981 ctcaggcgtc atggcggcgg cgttttccgc cgatttgtca tcgcaggcgg tgagcatcgc
     8041 gccgcagaat aacaacggta ttaacagctt tcttcttctg ttcattttag tccctgaaaa
     8101 ttcttgagaa ataacggttc tttaaattgc gcgcgaggag agatatgagg gatttacaga
     8161 ccagcggcat agtggggttg tcagccagta aggtgggata caggcacagt gatcgacatg
     8221 gtgaggtcaa cgacaaaaaa aatgttgccg ttctgccaac agttcctgcc agcattcggg
     8281 caacgaaggg ttctactggt ggatacacat acctggggaa taaagtcgtt ttagcctctc
     8341 ctttagtaat caccgggggt aatgaaatca gcatctgcat ccctcgtcat gccagccatc
     8401 aatttcagtt gcttatgtcc tgactaaaaa taagatgtga tacccagggt gacgatgtcg
     8461 ctcatacgtc ttagtttcag cttgtacata atgttgaatt tctgaatctt taatgtttgt
     8521 tgcgaaatat tgagcgtcga cgtgatattg ctttcatttt tatttttcag aatcagttgc
     8581 ataattttga actgattatg tgaaaaacat aattgtgaat tcttatgggg caagtgttta
     8641 ccataagtaa tctcaagaag atttttgata ctttctttgc ggctaacaaa ataaataggc
     8701 gctttagctt ttagtttact gatgtgataa ggtgccaaaa caataattct ctcggcatct
     8761 aatttctcca gaaatttaat cgcttcttca tcaaggatat gattgtgata ggtatcaatg
     8821 ataaccttga agggtttatt gcctactttt ttgatctctg acagggaatt gatttttatc
     8881 atttcgtgat tatctttcaa ctgccaaaag ccctgtaaaa gaaaagaatc tttcgtcatg
     8941 agaaaaatca taacttgctc cttagccgtt atcgtttcga acatgtcatc cttttcatta
     9001 tttacatcct tgtccgaatc gttgttcaat atagtaaacg cctataagaa ctttaccctt
     9061 cgtgaactaa tacgtctatt aacactaatc aatacttcag atttatccat cacgcactcc
     9121 atatattgat cctaagtaag attcctggtt gttatcagct tgtaaatagt aaacaaaaag
     9181 ttaacgtttg cggattactg cccaagaata agttatcaat aaccagtttg tgatctctga
     9241 agaatattac taaagttaaa atgtaatccg atttaaatat cgagtctcct tgtttcgact
     9301 taagctggca attggattgc cagctttctt ttaacgcatt gaaattacct gagtaaataa
     9361 cgcattcatg tcttcaggtt tttatatagc gtctggagac acggatattt atgcaatgta
     9421 atcaacgcca gttgcaaaaa aatgaacccg gattacttat ttaaccgccc atagcatcaa
     9481 cgcttacgga actctttgtt cactttccca cagcactttt aaaacattaa acctgactac
     9541 ggaaaatatc agccatgata atgtttgaaa tggctaattt gccatggagt gaaaaaaatt
     9601 agatgaaatt cagtaggttg aaataatcac tagcaggtaa ttatttcaat gatagtgcgc
     9661 aattgatcta caacactccg tagcggagag agtattaatc ggatcatagt catatcaagt
     9721 gactatgatc cgggtgacaa ccgggataat tattgctgct taacgaacaa actggcgaag
     9781 ctgaacaggc tggcggcgct aaatatcagt tcaattccca ccagtgtgga aaccagcgtt
     9841 acagacacca tcggcgttgc accaaggaat atccaggcaa tgacgatatc cagcacacca
     9901 ataacgagct gtagccagct gcctttcatt gaacgctgac gataccaact catcaggcga
     9961 ataacccctg caacacagaa caaaccggca ataaatgccg caatggcaaa aatgcccagc
    10021 tccggtgcgc ggatgaagaa atagccgatc aataaatagg cgactgcgac gaggaaaccg
    10081 gataataccg gccagaaatt atgactgcgg ttgctgaata acccgacaat aagcgcaatg
    10141 cccgagcaga ttaataatgc acccactact gtgcttaaaa tatcgccaga gacgaacggg
    10201 aaactgatac acagcaaccc gacgataaac agtagcacgg caataaactg gattgctctg
    10261 cgatgttttt taagcatctc cagatcaaac ttcaaaattg ttgccttatc tatatataac
    10321 atagaaccac cctataaaat taagaagaaa atcccctgct atcaatctat gccaaaaacg
    10381 cgtctaagaa tgtagtcgat ttaataaaaa tttcctaatt acagtatctg atgcatctgt
    10441 aactcattgt attgaaataa aaatatctga ttttgatatt ttatatcaac atgacatata
    10501 cagaaaacca ggttataacc tcagtgtcga aattgattcg tgacggctct ttcactttat
    10561 agttgaggat attacgatga aaaaagtatt aggcgttatt cttggtggtc tgcttcttct
    10621 gccagttgtg agcaatgcag cggatgcgca aaaagcagct gataacaaaa aaccggtcaa
    10681 ctcctggacc tgtgaagatt tcctggctgt ggacgaatcc ttccagccaa ctgcagttgg
    10741 ttttgctgaa gcgctgaaca acaaagataa accagaagat gcggttttag atgttcaggg
    10801 tattgcaacc gtaaccccag ctatcgttca ggcttgtact caggataaac aagccaactt
    10861 taaagataaa gttaaaggcg aatgggacaa aattaagaaa gatatgtaat tccgggaatg
    10921 cgttacatcg tacttccttg catattgaac aggccggaat atcttcttta aaagcagcta
    10981 ttcctcctgt tcatatataa tctctatatt gaatgggtta caaaatgaat atttcatctc
    11041 tccgtaaagc gtttattttt atgggcgctg tagcggcttt gtcactggtg aacgcacaat
    11101 ctgcgctggc agccaatgaa tccgctaaag atatgacctg ccaggaattt attgatctga
    11161 atccaaaagc aatgaccccg gttgcatggt ggatgctgca tgaagaaaca gtatataaag
    11221 gtggcgatac cgttacttta aatgaaaccg atctcactca aattcctaaa gtgatcgaat
    11281 actgtaagaa aaacccgcag aaaaatttgt ataccttcaa aaatcaagca tctaatgact
    11341 tgccgaatta atgaggtaca agtaaaaagg agtagcaagt tgagccatct tgctgctcct
    11401 ttttgcattt ttatatgaca gcagaattta ttatacgtct tatactcgcg gcaattgcct
    11461 gtggcgctat tggcatggaa aggcaaatgc gcggcaaagg agcagggtta cgcacacatg
    11521 tattaattgg catgggaagc gccctgttta tgattgtttc gaaatatggt tttgctgacg
    11581 tgctgtcttt agatcacgtc ggactcgacc ccagccgtat tgctgctcag gtggtgacgg
    11641 gcgtcgggtt tatcggtgca ggtaacattc tcgttcgtaa ccaaaatatt gtaggtctga
    11701 cgacggcagc ggatatctgg gtgaccgccg ccataggtat ggttattggc agcggtatgt
    11761 acgaactggg tatttatggt tcagtgatga ccttgttggt gctggaagtc ttccatcaat
    11821 taaccttccg cctgatgaac aaaaattacc atttacagct gacattagtg aacggtaata
    11881 tggtatcaat gctcgactgg tttaagcagc aaaaaataaa aactgattta gtttcgttgc
    11941 aggaaaatga agatcatgaa gtggtcgcca ttgatatcca gcttcatgcc acaacgtcga
    12001 tagaagacct gttgcgatta ttaaaaggta tggcaggggt gaaaggcgtt tcaattagct
    12061 aatctgtgtg gcagcgtagc cagactcacc gtaagcctga aattcacacc agataatcaa
    12121 tatgctgatg gcgtaccagc tcattgatcc gcttaagatt gaggatcatc atgatattgt
    12181 gtttatggca gtagaacgtt tttagcgata ttttcagttt ctccagaata tcttcagttg
    12241 tcatttgatt cgcaatgcag ctcagaacct gatgttgctt gcgcgaaagt gacacattcg
    12301 cttcaggcac agagcagtaa ctgccattga tgagtgcgac aaaatcaatg atagtgatag
    12361 tccgcggaac aaaggtcacc ggtactttcc ccagacagcg tttaatatta aagggggaga
    12421 aaacaatgac atggacaggt tttaatcgtt caatttgagt gagaaaatta gaatgaaata
    12481 cattattcat taatgtatca ataatgacga aggcattctg atgcaacatt acgtcgatct
    12541 cttcttcgtt ctgtatatga acgacattac ctttactcag aatgtttttc atcgcggtga
    12601 agaacatcgt atccctggta attataagaa acataaccga ctcctggtgc ggacattaac
    12661 tttataatca atttgccaaa ggtaattaac aggacagggc ttataataat aatagttagc
    12721 ttactgtata ttaattttat gtaatacatg atgcagatgc tgagaaagca tctgcatctt
    12781 tcggtggtgt tatttgacca gctcaggtgt tacctgactc accgcattgg tgtagtaagg
    12841 cgcaccccag cccggttccg gccagaatgc accatagccg taatcccaca ggttataggt
    12901 ggtattaacc acttctctca aatgccacac ctggatgccc tgcatattca cttccaggaa
    12961 gttatacggg actttattga taaagccagg ttgttcaccc tgaatggtac cgaggatggt
    13021 aacaaagtga ttacgatagt tcactgggtc aaggaagccg ctttgtctgg cgagcagtcg
    13081 gccctgatag ttggcttcaa tatcaggttt cgcatagcta tccaacggta atacagcgat
    13141 ttctaacaac gtatccgttt tgccattgat aacgttgata accttcccac caaagcgcgc
    13201 ttgttgacca acatataacc ccggctggtt atgaacagca acaaaacttt tttgaatatc
    13261 aggttggtta ttgcctttga tattttgcgg aattgaacta catgcggcaa gcaaaaatga
    13321 aaggctgagg atgagtgcac cttttgtcat gttcatgtta ctatccttat caacaaatat
    13381 taaaaccata atagatattt ctttatagtt ttcatctgat tctgaggcga tatgcctgat
    13441 agtaacgtaa acctgtctga tgtttatatc ccttttgttt agttcaattc cattgatttt
    13501 ggggcgtggc atgagtatcc atcgccccat aatataatcc
//
LOCUS       DAESHI010000118        13265 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.119, whole genome shotgun sequence.
ACCESSION   DAESHI010000118 DAESHI010000000
VERSION     DAESHI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13265
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.119"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(897..1988)
                     /gene="ychF"
                     /locus_tag="KW011_003441"
     CDS             complement(897..1988)
                     /gene="ychF"
                     /locus_tag="KW011_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBH7661697.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(2105..2689)
                     /gene="pth"
                     /locus_tag="KW011_003442"
     CDS             complement(2105..2689)
                     /gene="pth"
                     /locus_tag="KW011_003442"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBH7661698.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2966..3244
                     /gene="ychH"
                     /locus_tag="KW011_003443"
     CDS             2966..3244
                     /gene="ychH"
                     /locus_tag="KW011_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HBH7661699.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(3299..4978)
                     /gene="dauA"
                     /locus_tag="KW011_003444"
     CDS             complement(3299..4978)
                     /gene="dauA"
                     /locus_tag="KW011_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HBH7661700.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(5102..6049)
                     /gene="prs"
                     /locus_tag="KW011_003445"
     CDS             complement(5102..6049)
                     /gene="prs"
                     /locus_tag="KW011_003445"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBH7661701.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(6200..7051)
                     /gene="ispE"
                     /locus_tag="KW011_003446"
     CDS             complement(6200..7051)
                     /gene="ispE"
                     /locus_tag="KW011_003446"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBH7661702.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(7051..7674)
                     /gene="lolB"
                     /locus_tag="KW011_003447"
     CDS             complement(7051..7674)
                     /gene="lolB"
                     /locus_tag="KW011_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HBH7661703.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7888..9144
                     /gene="hemA"
                     /locus_tag="KW011_003448"
     CDS             7888..9144
                     /gene="hemA"
                     /locus_tag="KW011_003448"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HBH7661704.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            9186..10268
                     /gene="prfA"
                     /locus_tag="KW011_003449"
     CDS             9186..10268
                     /gene="prfA"
                     /locus_tag="KW011_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBH7661705.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            10268..11101
                     /gene="prmC"
                     /locus_tag="KW011_003450"
     CDS             10268..11101
                     /gene="prmC"
                     /locus_tag="KW011_003450"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBH7661706.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            11098..11490
                     /locus_tag="KW011_003451"
     CDS             11098..11490
                     /locus_tag="KW011_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HBH7661707.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11494..12303
                     /locus_tag="KW011_003452"
     CDS             11494..12303
                     /locus_tag="KW011_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HBH7661708.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            12339..13193
                     /gene="kdsA"
                     /locus_tag="KW011_003453"
     CDS             12339..13193
                     /gene="kdsA"
                     /locus_tag="KW011_003453"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HBH7661709.1"
                     /translation="MKQKVVSIGDINVANDLSFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3347 a         3283 c         3395 g         3240 t
ORIGIN      
        1 ttaattcagc gagacgatct gctttggtat tcccattgtg ttgtttgatg cccataccac
       61 tcctatatag tacccagact atgggtctat ggattttgtc attttgttta aatcaatgaa
      121 taatatcctc ttatcatcat gatatttgta tggtgcaatc atgatgagct tctcagagaa
      181 taattctcta ttaccgggca aaatcaggaa cacaggaatt aatatatatt taacatcccc
      241 cttacaaagg tgagtttata tcgagttatt ccctaagttg tcgttactta ttccaattcg
      301 ttactataaa tatggcaaaa atattacaac aaacagcggt taaaaaattt ggtgaagatt
      361 ttttgtaata atcctaatta gttatgggaa tttcgcttga tgtatctgca taacaaaaac
      421 aatatgcgcc atttttgcat tactaataag aaaaagcaaa aaacatcgat attcattttt
      481 ggtcaatggg ttttctggcc tatcgttgtt attgtgctac tggttatcat tttcatcttg
      541 ccaggatcgc acgtaatcac gttattcggg gactttatct gcgggatatt acccttcagg
      601 taagtccttt tctcgccagc atattggagc gtgatcaata ttgatcagct gtgaaggggg
      661 tgatgtgtag ttgaacaaaa acttacgtta tatcactact aatgagatat ccgtcatctg
      721 acactgatgt ctttaattat tctcgtggtt cactgtaggc aataagcaca aaagtgtagg
      781 atgttacaag aatgattagg actcggtgaa atgaaaaatc cacgcaattg cgtggatttt
      841 atatactttt gcgctcttca tgagatttag cgaaacctca tgagacaata aattaattag
      901 acgttgaaaa ggaagttcat cacatcgcca tctttaacga tgtaatcttt accttctgca
      961 cgcattttgc ctgcttcttt cgcgccttgt tcacctttgt aagtgatgaa atcttcaaac
     1021 gagatggttt gtgcacggat aaagcctttt tcaaaatcag tatggatttt gcccgctgcc
     1081 tgcggcgcgg ttgctccaac cggaatggtc catgcacgca cttctttcac cccagcggtg
     1141 aagtaagttt gcaggttcag cagcttataa ccgacacgga tcacacggtt caggcccggt
     1201 tcttccagcc caagctcctg cataaactcg tcacgttctt cgtcgtccag ttcggcaatg
     1261 tctgcttcaa cagcagcaca aaccggaacc acaacagaac cttctttcgc cgcgatttca
     1321 cgcacctggt caagatatgg gttgttttca aaaccgtctt cgttgacgtt ggcgatgtac
     1381 attgttggtt tcagcgtcag gaagctcagg taacgaatag ccgctttctc ttcagcgctt
     1441 aaatccagcg cgcgcagcat acctgcgttt tccagctggg gcaggcattt ttctaggacc
     1501 gccagctcag ctttcgcgtc tttatcgcca cctttggctt tcttctgtac gcgatgaatc
     1561 gcacgttcgc aggtgtcgag gtctgccagc gccagttcgg tgttgataac ttcaatatcg
     1621 tcagccgggt taactttgcc ggaaacgtga atgatgttgt cattttcaaa gcagcgaaca
     1681 acgtgaccga tcgcttcggt ttcacggatg ttggtcagga actggttacc cagaccttcg
     1741 cctttcgatg cgccttttac cagaccggcg atatcgacaa attccatggt cgtgggaagc
     1801 gtacgttgcg gttttacgat ttcagccagt tgatccaggc gagggtcagg cattggtacg
     1861 acgcctgtgt tcggctcaat ggtgcagaat ggaaagttgg ccgcttcaat accggctttg
     1921 gtcagcgcgt tgaacagggt agatttcccg acgttgggca aaccgacgat accgcatttg
     1981 aatcccatga tttaaatcac cttaatatct taataatcaa tctgttattg ctaacagatt
     2041 gcagaaatgg aaataacttt gcctattata cacggcactc ggcaaaaatg ccgcagacaa
     2101 cgacttattg cgctttaaag gcgtgcaatc ggttcgttgc tttggtcaag ccatctgtaa
     2161 accacatttc agtacaacgc gccgcttcgt cgatggcttc atcaattaac ttctgttcac
     2221 taacaggcgg tttgcctaac acaaaaccga caactttatt tttatcgcca ggatgaccga
     2281 ttccgatgcg taaacggtga aagttagggt tattacccaa tttactgatg atgtctttca
     2341 gtccattgtg accaccatgg ccaccgccca atttaaattt ggcgacgcca ggaggcagat
     2401 ccagttcgtc gtgggccacc agaatttcgt ccggattaat gcggaaaaaa ctggccatcg
     2461 ccgcaacggc tttgccgctg agattcataa atgtagtcgg gactaacagg cggacatctt
     2521 cgcctccaag agtgactcgc gaagtataac caaagaattt agcctcttcg cgcagcggag
     2581 cgcgcaaacg ctctgccagt aagtcaacga accaggcacc agcattatgt cgcgttgcgg
     2641 cgtattcagc accggggttc gccaggccga caatcaattt aatcgtcacg ttttttgtcc
     2701 tgagtgtgta cataactggc gcgtagttta ctggttgcgg ccccgcttga caaaaaactg
     2761 cgtatcaaat gcagataacg taataattgc ctgagtggac tattagaaag tcaaggtgtt
     2821 caggcgttta tttgtaaagt tttgttgaaa taagggttgt aattgtgatc acgcccgcac
     2881 ataacccaca gggtgttgtc tatactttac acataaggaa gaggggtatt ccctgttaca
     2941 acccagaaag ttccggaggt gacatatgaa acgcaaaaac gcttcgttac tcggtaacgt
     3001 gctcatgggg ttggggctgg tggtaatggt ggtcggcgtg gggtattcaa tcctcaacca
     3061 gttaccacag tttaatatgc cccagtattt cgcacatggt gcagtgctaa gtattttcgt
     3121 cggtgccatt ctctggctgg cgggtgcccg tgttggcggg catgaacagg tgtgcgaccg
     3181 ttactggtgg gttcgtcact atgacaaacg ttgccgccgt agcgataatc gccgtcatag
     3241 ctaacaaata atgcagtttg ctgaccagtc agtttgcgct gactggtcaa tctcgtactt
     3301 ataaatccgc catcgccgcg cgacgattcg ggaagaacgc gaggcgtccc gggatcggtt
     3361 gaatgcctgc gcgagccata gtgcgcagtg gctggaattc cacgttgcac acgcgcagtt
     3421 cacatccttc gggcagacgc ttcacaaaac gctggaacgc atcaagacca ccagcatcaa
     3481 gtaccggaac ggcatcccac ttcagaatca caatccgttt gccttcaaga cgtgactcca
     3541 ggtccgtgaa caagccttca gcagcagcaa aaaacagcgg gccaataacg cgcagtacca
     3601 gaacatcgtc tggaacatct acgactaccg gtgccaggcg agtcatacgt gcgatacgac
     3661 gcataaacag cagcgatgcc agcacgatcc ccacgctgat ggcaataacc atatcaaaca
     3721 gcacggtcag cgacatgcac agcagcatga caatgatgtc atctttcggt gcatgacgca
     3781 gcaagtcgac cactttatgc gcttcactca tgttccacgc caccatcaac agcagggctg
     3841 ccatagcgga aagcggcagc caggagagca gcggtgccag taccagcagg gcaagaataa
     3901 ccagaataga gtggatcacc gccgagatag gggaagttgc cccggcacgg acgttagcgg
     3961 cagaacgcgc gatggcagct gtagcggtaa taccaccaaa gaacggagcg ataatattcc
     4021 ccagtccctg tccaaccagt tcgctgttcg ccttgtgttt cgtcccggtc ataccatcca
     4081 gtaccacggc gcagagcaga gattcgattg cgccgagcat tgccattgag aatgccgcag
     4141 gcagcagtgt gcgaatagaa tcccaggtta gcgtgaattc tgaattaggc agatcccacg
     4201 gcagcaccag ttgcggcagc agttgcggaa taccgttacc ctgagaacca tcggccagga
     4261 cgtagtggaa ttgcgaaccg atggtagcaa catgtccgcc gagcaggtta acaatcccca
     4321 tcaccgcgca accagccagc aaggccggaa ggtgaccggg taaacgaatg cccagacgcg
     4381 gccaaaaaac aagaatacct agcgtcacaa tgccaatggc agcatcaccc acattaatgg
     4441 tcggcagcgc cataaataat gcgccgactt tttgtagata atgttccggg acatgggcca
     4501 tttgcagacc gagaaaatct ttaatctgca tggtaccgat ggtgatcccg atacccgagg
     4561 tgaaacctaa ggtgacggaa accggaatat actcaatcag gcgaccaaag cgtgccagac
     4621 ccatcagaat caaaaagatc cccgacagca aggtcgcaac cagcagtcct gccagtccaa
     4681 actgttgcga cacgggatag agaattacca caaatgccgc agtcggaccg gaaacgctaa
     4741 agcgtgaccc acccgtcaga gcaatgacaa tccccgcaac agctgcggta tataaaccgt
     4801 actggggtgc cacaccacta ccaatagcca acgccatcgc cagcgggata gcaataatcc
     4861 cgacggttat cccggcaatc aggtcacggg taaaccgtgc ggcagtatat ttttctttcc
     4921 agcaagcgtc gatcagagcg cggaaaggca tcacatgtga ggaaaatatt ttgttcacaa
     4981 taatgtttca tccgtgagcg catcatctgt caactaaatg gcaggtgaag gaggcatagg
     5041 tcatacaaat ggatattaca gacaaaaaaa cccgccgcag cgggtctttg agcgggttcg
     5101 attagtgttc gaacatggca gagatcgatt cttcgttgct gatacgacga atcgcttcgg
     5161 ccagcatacc tgacagggtc agagtacgca cgttcggcag tgatttgatt tcatcgctca
     5221 gcggaatggt atcgcagaca acgacttcat caattacaga gttacgcagg ttgttcgccg
     5281 cgttgccaga gaagatcggg tgagtcgcgt acgcaaatac acgtttagca ccacgttctt
     5341 tcagagcttc agcagcttta cacagcgtac cgccagtgtc gatcatatca tcgaccagta
     5401 cgcagtcacg acctgcaacg tcaccgatga tatgcatcac ctgggaaacg ttcgcacgcg
     5461 gacgacgttt gtcgatgatt gccatatcgg tatcgttcag cagcttagcg atagcgcggg
     5521 cacgcacaac gccgccgatg tccggagaaa ccacaattgg gttatccaga ttcagctgca
     5581 gcatgtcttc cagcaggatc gggctaccaa atacgttatc aaccggaacg tcgaagaaac
     5641 cctgaatctg ttcagcgtgc agatccactg tcagcacacg gtcaacaccg acgctggaga
     5701 ggaagtctgc aaccactttc gcagtgattg gtacacgagc ggaacggacg cgacggtcct
     5761 ggcgcgcata gccaaagtag gggataacag cggtgatacg acctgcggaa gcacgacgca
     5821 gggcatcaac cataacgact aattccatca ggttgtcgtt agtaggggca caagtggact
     5881 ggatgatgaa aatatcacca ccgcgtacat tttcgttgat ttgtacactg acttcgccgt
     5941 cgctaaagcg acctacagcg gcgtcgccga gtgaagtgta caggcggttg gcaatacgtt
     6001 gtgctagttc cggggtggcg ttaccagcaa aaagcttcat atcaggcacg agaagaacct
     6061 caggcatgcg tccattggtg gaaagaatct gccgaaaact gtgcgggcca ggcatgatcc
     6121 tttccaggcg gtgtattaaa gagcgcgatg caacgtctgg aacaaggtga cgttgtcacc
     6181 gaaactcagc ttgcccggct taaagcatgg ctctgtgcaa tggggaaaga ttaacacctt
     6241 tcgccacaaa gccattgagc cattccgggg cttgctctag cacctggcgg gcttcagact
     6301 ctgtatcaaa ttcagcaaag acacaggccc ctgtcccagt caggcgcgac ggggcgtatt
     6361 ctaacagcca ggaaagcacc gcatcaacct cgcgaaaacg ttttcttgcg ataacctcgc
     6421 aatcattgct gaattcacat tttagcaacg tttctattga cctttttggc gtattgcgcg
     6481 ggagttcagg atctttaaaa atcactggtg tcggaatact tacaccaggg tgcgccacca
     6541 gataccactt ctctggcgga tccaccggcg ttagtatttc accaacgcct tcggcaaacg
     6601 cggcatgccc ccgaacaaag acaggaacat ctgcgcccag cgtcagcccc atttccgcca
     6661 gctcatccat gcttagcccg cattgccaga gatgatttaa tgccaccagg accgtcgcgg
     6721 cattggatga accaccgccg agaccgccgc ccatcggcaa acgcttgtca atgctgatat
     6781 tcgcaccgct tcccgtcgga agacgcccgc tgtctgccgc agttttcatc aacaaccgcg
     6841 ctgcgcgaac gatcaggtta tcttcatgtt ccacgccttc aacgggcgtt aacagacgaa
     6901 tatccccatc gtcacgaagc tcaatgctga tggtgtcgcc gtaatcaaga aactgaaaca
     6961 gcgtttgcag cgtgtggtaa ccatccgcac gctgaccggt aatgtataaa aacagattaa
     7021 gttttgccgg agagggccac tgtgtccgca ttatttcact atccagttat ccatttttaa
     7081 cttgatgcgt tgaccaccgt cggtgagttc catattggct ggcatcgcag gttgcgtttt
     7141 ggtgtcataa ccaccataaa caaccttcca gtttttgcca ttctggctgt aggtaatttc
     7201 gctcaggcgg tactggtcgt ccagtttgta gtcggttgca tcacccggta aacctaaaat
     7261 ccattggcgc aagctgttga gcggaattgg cattccggtc aatttgccaa tcatctcttc
     7321 ggcgtcatcg gcggtataac gctgaccttt attgtcgact aactgcacgt tacccggttg
     7381 agcattcagc tccagttccg tgctgcccaa tgggttagtg agcagcagac ggtagcgatc
     7441 ctggccggtt tgctgccaga aaaagcgggc gtacactttt tgttggtcag aaatataagc
     7501 gaacgcgccg cgagtctgat actgattaag attgcgcacg tcttgctgat gctgacgcca
     7561 ttgtggcgaa tccgggcttt tgccaggacc tttgggcgtg gtaacggaac aggcagtgag
     7621 cacaagagca gccagcggta gcaggcggat aagacgaaaa tcgggcaggg gcatagtgat
     7681 gacaagtcct tgagatacgt tgcagttata acccttaatg ctagcgttac cgtccgctat
     7741 cgtctatgtt caagttgtct taattgccag aatctaacgg ctttcggcaa ttactccaaa
     7801 agggggcgct ctcttttatt gatcttacgc atcctgtatg atgcaagcag actaaccata
     7861 tcaacgttgg tattatttcc cgcagacatg acccttttag cactcggtat caaccataaa
     7921 acggcacctg tatcgctgcg agaacgtgta tcgttttcgc cggataagct cgatcaggcg
     7981 cttgacagcc tgcttgcgca gccgatggtg cagggcggcg tggtgctgtc gacgtgcaac
     8041 cgcacggaac tttatcttag cgttgaagag caggataacc tgcaagaggc gttaatccgc
     8101 tggctttgcg attatcacaa tcttaatgaa gaagatctgc gtaaaagcct ctactggcat
     8161 caggataacg acgcggttag ccatttaatg cgtgttgcca gcggcctgga ttcactggtt
     8221 cttggggagc cgcagatcct cggtcaggtt aaaaaagcgt ttgccgattc gcaaaaaggt
     8281 catatgaagg ccagcgaact ggaacgcatg ttccagaaat ctttctctgt agcgaaacgc
     8341 gttcgcactg aaacagatat cggtgccagt gctgtgtctg tcgcttttgc ggcttgtacg
     8401 ctggcgcggc agatctttga atcgctctct acggtcacag tgttgctggt aggcgcgggc
     8461 gaaaccatcg agctggtagc gcgtcatctg cgcgaacata aagtacagaa gatgattatc
     8521 gccaaccgca ctcgcgaacg tgcccaaatt ctggcagatg aagttggcgc ggaagtgatt
     8581 gccctgagtg agatcgacga acgtctgcgc gaagccgata tcatcatcag ttccaccgcc
     8641 agcccgttac cgattatcgg gaaaggcatg gtggagcgcg cattaaaaag tcgtcgtaac
     8701 caaccaatgc tgttggtgga tattgccgtt ccgcgcgatg ttgagccgga agttggcaaa
     8761 ctggcgaatg cttatcttta tagtgtggac gatctgcaaa gcatcatttc gcacaacctg
     8821 gcgcagcgta aagccgcagc ggttgaggcg gaaactattg tcgctcagga aaccagcgaa
     8881 tttatggcgt ggctgcgagc acaaagcgcc agcgaaacca ttcgcgagta tcgcagccag
     8941 gcagagcacg ttcgcgatga gttaaccgcc aaagcgttag cggcccttga gcagggcggc
     9001 gacgcgcaag ccattatgca ggatctggca tggaaactga ctaaccgctt gatccatgcg
     9061 ccaacgaaat cacttcaaca ggccgcccgt gacggggata acgaacgcct gaatattctg
     9121 cgcgacagcc tcgggctgga gtagcagtac atcattttct ttttttacag ggtgcattta
     9181 cgcctatgaa gccttctatc gttgccaaac tggaagccct gcatgaacgc catgaagaag
     9241 ttcaggcgtt gctgggtgac gcgcaaacta tcgccgacca ggaacgtttt cgcgcattat
     9301 cacgcgaata tgcgcagtta agtgatgttt cgcgctgttt taccgactgg caacaggttc
     9361 aggaagatat cgaaaccgca cagatgatgc tcgatgatcc tgaaatgcgt gagatggcgc
     9421 aggatgaact gcgcgaagct aaagaaaaaa gcgagcaact ggaacagcaa ttacaggttc
     9481 tgttactgcc aaaagatcct gatgacgaac gtaacgcctt cctcgaagtc cgagccggaa
     9541 ccggcggcga cgaagcggcg ctgttcgcgg gcgatctgtt ccgtatgtac agccgttatg
     9601 ccgaagcccg ccgctggcgg gtagaaatca tgagcgccag cgagggtgaa catggtggtt
     9661 ataaagagat catcgccaaa attagcggtg atggtgtgta tggtcgtctg aaatttgaat
     9721 ccggcggtca tcgcgtgcag cgtgttcctg ctacggaatc gcagggtcgt attcatactt
     9781 ctgcttgtac cgttgcggta atgccagaac tgcctgacgc agaactgccg gacatcaacc
     9841 cagcagattt acgcattgat actttccgct cgtcaggggc gggtggtcag cacgttaaca
     9901 ccaccgattc ggcaattcgt attactcact tgccgaccgg gattgttgtt gaatgtcagg
     9961 acgaacgttc acaacataaa aacaaagcta aagcactttc tgtacttggt gctcgtatcc
    10021 acgctgcaga aatggcaaaa cggcaacagg ccgaagcgtc tacccgtcgt aacctgctgg
    10081 ggagtggcga tcgcagcgac cgtaaccgta cttacaactt cccgcagggg cgcgttaccg
    10141 atcaccgcat caacctgacg ctctaccgtc tggatgaagt gatggaaggt aagctggata
    10201 tgctgattga accgattatc caggaacatc aggccgacca actggcggcg ttgtccgagc
    10261 aggaataatg gaatatcaat actggttacg tgaagcaata agccaacttc aggcgagcga
    10321 aagcccacgc cgtgacgctg aaatcttatt ggagtatgtc actggcaggg ggcggacttt
    10381 tattctggcc tttggtgaaa cgcagctgac tgacgaacaa tgtcagcaac ttgatgcgct
    10441 actgacacgt cgtcgcgatg gtgaacccat tgctcattta accggggtgc gagaattctg
    10501 gtcgttgccg ttatttgttt cgccagcgac cttaattccg cgcccggata cggagtgtct
    10561 ggtggagcag gcactggcgc ggttgcctga acaaccttgc cgtattctcg atctcgggac
    10621 gggtaccggg gcgattgcgc ttgcgctggc tagcgagcgc ccggactgcg aaattatcgc
    10681 tgtagatcgt atgcctgatg ctgtctccct ggcacaacgt aatgcccagc atctggcgat
    10741 caaaaatatc cacattctgc aaagcgactg gtttagcgcg ctagccgggc agcagtttgc
    10801 gatgattgtc agcaatccgc cgtatattga cgagcaggac ccacatcttc aacaaggcga
    10861 tgtccgcttt gagccgctca ctgcgctggt tgcggcagac agtggaatgg cagacatcgt
    10921 gcatatcatc gaacagtcgc gtaacgcgct ggtatccggc ggctttctgc ttctggaaca
    10981 tggctggcag cagggcgaag cggtgcgaca agcatttatc ctcgcggggt atcatgacgt
    11041 cgaaacctgc cgtgactatg gtgataacga gcgcgtaacg ctcggccgct attatcaatg
    11101 acaagttttt ctacactgct tagtgttcat cttattagta tcgcgctttc tgttgggcta
    11161 ttaaccttac gtttctggct acgttatcag aagcatccac aggcatttgc tcgctggacg
    11221 cgcattgtgc cgccggttgt cgatacggtg ttattgttaa gcggcattgc gttgatggct
    11281 aaagcgcaca tcctgccatt ttccgggcag gcacagtggc tgactgaaaa gctgtttgga
    11341 gttatcattt atatcgtttt gggttttatt gcactcgatt atcgtcgtat gcacagtcag
    11401 caggcgcgca ttattgcctt cccgctggcg ttggtggtgc tgtacatcat cattaaactc
    11461 gccaccacaa aagtaccgtt actggggtaa gtcatgagat cgttagctga tttcgaattt
    11521 aataaagcgc cattgtgcga aggcatgatc ctggcttgcg aagcaatccg ccgcgatttt
    11581 ccctcgcaag atgtttacga cgaactggag cgtctcgtta gtctggcgaa ggaagaaatc
    11641 agccagcttc tgcctttaga agagcagctg gaaaaactga tcgcgctgtt ttacggcgac
    11701 tggggattta aagcctcacg cggcgtttat cgtctttccg atgcattatg gctggaccag
    11761 gtgttaaaga atcgacaggg cagtgcggta tcattaggtg cggttttatt atgggtcgcg
    11821 aatcgtctcg atttgccgct gctgccggtg attttcccta cgcagctgat attgcgcatt
    11881 gaatgtccgg atggcgaaat ttggctgatt aatcctttta acggtgaatc gttaagcgaa
    11941 catatgctgg acgtatggtt aaagggaaat atcagcccgt cggcggaact gttttatgaa
    12001 gaccttgatg aagctgataa cattgaggta atccgcaaat tgttggatac actcaaagcc
    12061 tcgttgatgg aagaaaatca gatggagctg gcgttacgca ccagcgaagc tttattacaa
    12121 ttcaacccgg aagatcccta tgaaattcgc gatcgcgggt tgatttatgc gcaactggat
    12181 tgcgaacacg ttgcgttgaa cgatttaagt tatttcgttg aacagtgtcc ggaagacccg
    12241 atcagcgaaa tgatccgtgc gcaaataaat aacatcgcgc ataaacatat tgtgctgcat
    12301 taattaatcg acattttact caagattaag gcgatcctat gaaacaaaaa gtggttagca
    12361 ttggcgacat caacgtagca aatgacctgt cgttcgtact gtttggtggt atgaacgtgc
    12421 tggaatctcg cgatctggcg atgcgcattt gcgagcacta cgtaaccgtg acccagaaac
    12481 tgggtatccc ttacgtgttc aaagcctctt ttgacaaagc caaccgctcc tccatccact
    12541 cttatcgtgg accgggcctg gaagaaggga tgaaaatctt ccaggagttg aagcagactt
    12601 ttggcgtgaa aattatcacc gacgttcacg aaccaagtca ggcacagccc gttgctgatg
    12661 tcgtggatgt gattcagttg ccggcgtttc ttgctcgcca gactgacctg gttgaagcca
    12721 tggcgaaaac cggtgcggta attaacgtca agaaaccaca gtttgtcagc ccgggacaga
    12781 tgggtaatat cgttgataaa ttcaaagaag gcggcaacga aaaagtgatt ctttgcgatc
    12841 gcggtgctaa cttcggctat gacaacctgg ttgtcgatat gctgggcttc agcattatga
    12901 agaaagtgtc tggtaactcg ccggtgattt tcgacgtgac ccacgcactg caatgccgcg
    12961 atccgtttgg cgcagcttcc ggtggtcgtc gtgctcaggt ggctgagctg gcacgagccg
    13021 gtatggcggt aggtctggcg gggctgttta ttgaagcgca tccggatccg gaacatgcga
    13081 aatgtgatgg tccatccgcg ctgccgctgg ctaaactgga accgttcctc aagcagatga
    13141 aagcgattga tgatctggtg aaaggtttcg aagaactgga taccagcaag taatcttttt
    13201 tgcttgaaaa ataaagtctt agcgttctgc gttaagactt ttttcatggg tgccagatac
    13261 aaaaa
//
LOCUS       DAESHI010000119        13111 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.120, whole genome shotgun sequence.
ACCESSION   DAESHI010000119 DAESHI010000000
VERSION     DAESHI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13111
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.120"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            138..437
                     /locus_tag="KW011_003454"
     CDS             138..437
                     /locus_tag="KW011_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661710.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(484..1371)
                     /gene="rarD"
                     /locus_tag="KW011_003455"
     CDS             complement(484..1371)
                     /gene="rarD"
                     /locus_tag="KW011_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HBH7661711.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1423..1890)
                     /locus_tag="KW011_003456"
     CDS             complement(1423..1890)
                     /locus_tag="KW011_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HBH7661712.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            2055..2924
                     /gene="pldA"
                     /locus_tag="KW011_003457"
     CDS             2055..2924
                     /gene="pldA"
                     /locus_tag="KW011_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HBH7661713.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3057..4886
                     /gene="recQ"
                     /locus_tag="KW011_003458"
     CDS             3057..4886
                     /gene="recQ"
                     /locus_tag="KW011_003458"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBH7661714.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4950..5567
                     /gene="rhtC"
                     /locus_tag="KW011_003459"
     CDS             4950..5567
                     /gene="rhtC"
                     /locus_tag="KW011_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HBH7661715.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5629..6249)
                     /gene="rhtB"
                     /locus_tag="KW011_003460"
     CDS             complement(5629..6249)
                     /gene="rhtB"
                     /locus_tag="KW011_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HBH7661716.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6360..7382
                     /gene="pldB"
                     /locus_tag="KW011_003461"
     CDS             6360..7382
                     /gene="pldB"
                     /locus_tag="KW011_003461"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HBH7661717.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7390..8190
                     /gene="yigL"
                     /locus_tag="KW011_003462"
     CDS             7390..8190
                     /gene="yigL"
                     /locus_tag="KW011_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HBH7661718.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8266..9165
                     /gene="bioP"
                     /locus_tag="KW011_003463"
     CDS             8266..9165
                     /gene="bioP"
                     /locus_tag="KW011_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HBH7661719.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(9053..10006)
                     /gene="metR"
                     /locus_tag="KW011_003464"
     CDS             complement(9053..10006)
                     /gene="metR"
                     /locus_tag="KW011_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HBH7661720.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10242..12503
                     /gene="metE"
                     /locus_tag="KW011_003465"
     CDS             10242..12503
                     /gene="metE"
                     /locus_tag="KW011_003465"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBH7661721.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12788..13033)
                     /locus_tag="KW011_003466"
     CDS             complement(12788..13033)
                     /locus_tag="KW011_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH7661722.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         3104 a         3256 c         3492 g         3259 t
ORIGIN      
        1 atagcagtct ttgcattgat aactatacag tagtaattta gagtgtgtaa gtttttattg
       61 atggggtata ttttaattaa atataattga aatgtatttt tattctataa acaatatctt
      121 gggtgaatgg attctatatg ggaaatggta acttttggca gttgttcttg ggtaaagaac
      181 cttacagcaa aaaatctgat tttgttaagg catggttatt gtggtgggct attaccatcc
      241 ttgttctatt tattttaagc ctgactacga cattaaattt aagaagtata ggtatagggt
      301 taagttttgg gttttgtcaa atccttggta gatatacgat gtctctagga tggagtaagt
      361 actggggagt tttgggggta tttcctgtaa caaatattgt tctttttctt atcttaattt
      421 ttttcaaaaa aagataaata agtcagtggg taggctcttt aaacggccta cccatgttaa
      481 ttcttaactt tttctacgct gcgtataaat cgcatccatc acaaaaattg ccagcgccac
      541 ccaaataaag gcgaaagtca ccatcttatc ggcacccggt ttttcaccat aaaacgtcac
      601 agccagcagg aacatcagcg tcgggccaat gtactggaaa aagcctaacg ttgagagacg
      661 caagcgcgtg gcagcggcgg taaaacacaa cagcggtacg gtagtgacaa taccggcggc
      721 gatcagcagt aaattcagcg acatcgggtt ttgccccata tggctggttg agctgtcggc
      781 aatagcaaac aggtaaattg ccgccacggg cagcagccac atggtttcga ttaacatgcc
      841 ggtttgcgct tcaacggcaa tcttcttgcg taccagaccg tagaaggcaa aactaaatgc
      901 cagtcccagc gcgataatag gtagcgaacc aaaagtccac agctggacta acacgccaca
      961 tatcgccaga atcaccgcca gccattgcat ccggcggaat cgctcgccga ggaaaatcat
     1021 ccccagcaca atgttcacca gcgggttaat aaagtaacca aggctcgctt ccagcatatg
     1081 gtgattgttc accgcccaga taaacagtag ccagttgcca ccaatcagca cggcagagac
     1141 tgccagcata aaaattttct gtggcgtctg aatcagcgtt tttaaatagg accactggcg
     1201 gcaaatgctc atcagcacca ccataaagaa aaacgaccag atcacgcgat gcgtcaggat
     1261 ttcatcggcg ggcacgtagt aaatcaactt gaagtacgct ggcgctatac cccaaataaa
     1321 ataagcggca agagcgagta atacgccctg ccgcgtttgt tttgcatcca tcgggaatac
     1381 tcatttttaa ttggtaacag cagtttacct gcttttatgt cttcaaccta ccatataggt
     1441 ggcggtggca ctggcaatat aaagctgttc ttcattgtgt aattcaacgc gggcgacggc
     1501 gactttattg cctgcacgca acaggctact ggtagcagta aaacgctcgc ccctgcctgg
     1561 gcgcagataa tcaacgcgaa gatcaatggt ccccatccgc gatagccgct ggcgtagttc
     1621 atcttcactg atggtttcgt ggcgggttaa ggtacttccc acgcacacca gaccggcggc
     1681 gacatccagc gccgacgcaa tgaccccgcc gtgcaaaatg ctttgcgccc agttgcccac
     1741 catcattggc tgatttttaa aggccagctg tgcgaactct ttttcgtaac gctccagttc
     1801 catccccaat gcgcggttaa atggcatgtg ataaacaaac atctcaccca ctaatttcag
     1861 ggcttgttca gcggtcagta cggcagacat atcatcctta cacttcattg gttaatgaaa
     1921 tgttgatttt atgcttcttt gttgttggtt tctactttag gaagggataa ctaacggcta
     1981 tggagttaag tatgtaaaat agcccgcaga aaaatattca ccttatcaat aattcgttac
     2041 ggagaacacg accgatgcgg actctgcagg gctggttgtt gccggtgttt atgttgccta
     2101 tggcagtata tgcacaagag gcaacggtga aagaggtgca tgacgcgcca gcggtgcgtg
     2161 gcagtattat cgccaatatg ctgcaggagc atgacaatcc gttcacgctc tatccttatg
     2221 acaccaacta cctcatttac acccaaacca gcgatctgaa taaagaagcg attgccagtt
     2281 acgactgggc ggaaaatgcg cgtaaggatg aagtaaagtt tcagttgagc ctggcatttc
     2341 cgctgtggcg tgggatttta ggcccgaact cggtgttggg tgcgtcttat acgcaaaaat
     2401 cctggtggca actgtccaat agcgaagagt cttcaccgtt tcgtgaaacc aactacgaac
     2461 cgcaattgtt cctcggtttt gccaccgatt actgttttgc aggttggacg ctgcgcgatg
     2521 tggagatggg gtataaccac gactctaacg ggcgttccga cccgacctcc cgcagctgga
     2581 accgccttta tactcgcctg atggcagaaa acggtaactg gctggtagaa gtgaagccgt
     2641 ggtatgtggt gggtaatact gacgataacc cggatatcac caaatatatg ggttactacc
     2701 agcttaaaat cggctatcac ctcggtgatg cggtgttgag tgcgaaagga cagtacaact
     2761 ggaataccgg ttacggcggc gcggagttag gcttaagtta cccgatcacc aaacatgtgc
     2821 gcctttatac tcaggtttac agcggctatg gcgaatcgct catcgactat aacttcaacc
     2881 agacccgtgt cggtgtagga gttatgctaa acgacttgtt ttgatgaacg gttgagtggt
     2941 tggcaaatct ggaacccagc atccaggatt accctctcag agactaaaag cattgcagtt
     3001 tctcgcgcag gcgctgaaaa tagcgcctgt ttttatttca ggcaatcggg gtgaatgtgg
     3061 cgcaggcgga agtgttgaat ctggagtccg gagctaaaca ggttttacaa gaaacctttg
     3121 gctaccaaca gtttcgcccc ggccaggaag aaattatcga cactgtgctt tccggtcgcg
     3181 attgcctggt cgtcatgccc accggtggcg gaaaatccct ttgctatcaa attcctgcct
     3241 tattgctaaa cggccttacc gtggttgttt caccgctgat ttcgttgatg aaagatcagg
     3301 tggatcaact gcaagccaac ggcgtggcgg cggcgtgcct taactcgacg caaacccgcg
     3361 agcagcaact tgaagtgatg acaggctgcc gcaccgggca aattcgtctg ctttatatcg
     3421 ccccggaacg cctgatgctg gataactttc ttgagcatct ggcgcactgg aatccggtgt
     3481 tattagccgt tgatgaagcg cactgtatct cccaatgggg ccacgatttc cgcccggaat
     3541 atgccgcgct tggtcagtta cgccagcgtt tcccgaccct gccgtttatg gcgctgactg
     3601 ccacagccga cgacaccacg cgccaggata tcgtgcgctt gctggggctg aacgatccgc
     3661 tgattcaaat cagcagtttt gaccgtccga atattcgcta catgctgatg gagaaattca
     3721 aaccgctcga tcagttgatg cgctacgtgc aggaacagcg cggtaagtcc ggcattatct
     3781 actgcaacag ccgggcgaaa gtagaagaca ccgctgcgcg cctgcaaagc aagggtatta
     3841 gcgcggcggc ctatcatgcc gggctggaaa ataatgtccg cgccgacgtg caggagaaat
     3901 tccagcgcga tgacctgcaa attgtggtgg cgacggtggc gttcggcatg ggcatcaata
     3961 aaccaaacgt tcgcttcgtg gtccactttg atattccgcg caatatcgaa tcctattatc
     4021 aggaaaccgg tcgcgccggg cgtgatggcc tgcctgcgga agcgatgctg ttttacgatc
     4081 cggctgatat ggcgtggctg cgccgttgtc tggaagagaa gccgcagggg cagttgcagg
     4141 atattgagcg ccacaaactc aatgcgatgg gcgcgtttgc cgaagcgcaa acttgccgtc
     4201 gtctggtatt gctgaactat tttggcgaag ggcgtcagga gccgtgcggg aactgcgata
     4261 tctgcctcga tccgccgaaa cagtacgacg gttcaaccga tgctcagatt gccctttcca
     4321 ccattggtcg tgtgaatcag cggtttggga tgggttatgt ggtggaagtg attcgtggtg
     4381 ctaataacca gcgtatccgc gactatggtc atgacaaact gaaagtctat ggcatgggcc
     4441 gtgataaaag ccatgaacat tgggtgagcg tgatccgcca gctgattcac ctcggcctgg
     4501 tgacgcaaaa tattgcccag cattctgccc tacaactgac agaggccgcg cgcccggtgc
     4561 tgcgcggcga atcctctttg caacttgccg tgccgcgtat cgtggcgctc aaaccgaaag
     4621 cgatgcagaa atcgttcggc ggcaactatg atcgcaaact gttcgccaaa ttacgcaaac
     4681 tgcgtaaatc gattgccgat gaaagtaatg tcccgccgta cgtggtgttt aacgacgcaa
     4741 ccttgattga gatggctgaa cagatgccga tcaccgccag cgaaatgctc agcgttaacg
     4801 gcgttgggat gcgcaagctg gaacgctttg gtaaaccgtt tatggcgctg attcgtgcgc
     4861 atgtcgatgg cgacgacgaa gagtagtcag cagcataaaa aagtgccagg atgaagactc
     4921 cgtaaacgtt tcccccgcga gtcaaatgta tgttgatgtt atttctcacc gtcgccatgg
     4981 tgcacattgt ggcgcttatg agccccggtc ccgatttctt ttttgtctct cagaccgctg
     5041 tcagtcgttc ccgtaaagaa gcgatgatgg gcgtgctggg cattacctgc ggtgtaatgg
     5101 tttgggctgg gattgcgttg cttggcctgc atttgattat cgaaaaaatg gcctggctgc
     5161 atacgcttat ggttggcggt ggcctgtatc tctgctggat gggttaccag atgctgcgcg
     5221 gcgcgctgaa aaaagaggcg gtttctgcac ctgcgccaca ggtcgagctg gcgaaaagtg
     5281 ggcgcagttt cctgaaaggt ttactgacca atctcgctaa tccgaaagcg attatctact
     5341 ttggctcggt gttctcattg tttgtcggtg ataacgttgg cactaccgcg cgctggggca
     5401 tttttgcgct gatcattgtc gaaacgctgg cgtggtttat cgtcgttgcc agcctgtttg
     5461 ccctgccgca aatgcgccgt ggttatcaac gtctggcgaa gtggattgat ggttttgccg
     5521 gggcgttatt tgccggattt ggcattcatt tgattatttc gcggtgatgc cagacgcgtc
     5581 ttcagagtaa gtcggataag gcgtttacgc cgcatccgac attatttttc acgcatgcct
     5641 cgccgatgct aacagcgctc ccaccagcat aaacaacgag ccgaaaatct tattcagcgc
     5701 cttcatctgc tttggtcctt taatccatag agcaatccgt tgagcaaggg tggcgtaacc
     5761 gatcatcaca ataatatcga ccacaatagt ggtgacgccg agcacgatat actgcatcag
     5821 ttgcggctgt tgcggcatga tgaattgcgg aaatagcgcc gccagaaaca caatactttt
     5881 gggattggtg agattcacaa aaactgcgcg ctggaacaaa tgtcgacgcg attgagtaga
     5941 ggccagcgat ttaaggtcaa ttgcaccagc ggcgcgccac tgctggattc ccagccaaat
     6001 caagtaagcc gcgcctgccc acttcaacac ttcaaacgca atcactgagc gggaaaatag
     6061 cgtccccaac cccacgccaa ccagcacaat atgaatcgcc agtccggtct gaagcccagc
     6121 aatagacgcc accgcgccgc gataaccgtg gttgagcgag gtggtcatag tgttgattgc
     6181 accagagcct ggcgacaggc ttaaaatgat cgatgtcagc aggtaggcaa accaccattc
     6241 taaggtcatg atgaactccc ggtgtgtcta tttttgtgcc acaatacgct actgtcgcag
     6301 cgttgtgtca ggcacgctaa aaaaaacgat tttacgtggt ttaagaggca gattacccga
     6361 tgtttcagca gcaaaaagac tgggaaacaa gagaaaacgc gtttgctgct tttaccatgg
     6421 gaccgctgac tgatttctgg cgtcagcgtg atgaagcaga gtttactggt gtggatgaca
     6481 ttccggtgcg ctttgtccgt tttcgcgcac agcaccatga ccgggtggta gtcatctgcc
     6541 cggggcgtat tgagagctac gtaaaatatg cggaactggc ctatgacctg ttccatttgg
     6601 ggtttgatgt cttaatcatc gaccatcgcg ggcagggacg ttccggtcgc ctgttagccg
     6661 atccgcatct cgggcatgtt aatcgcttta atgattatgt tgatgatctg gcggcattct
     6721 ggcagcagga ggttcagccc ggtccgtggc gtaaacgcta tatactggca cattcgatgg
     6781 gcggtgcgat ctccacatta tttctgcaac gccatccagg tgtatgtgac gccattgcgc
     6841 taactgcgcc aatgtttggg atcgtgattc gtatgccgtc atttatggca cggcagatcc
     6901 tcaactgggc cgaagcgcat ccacgtttcc gtgatggcta tgcaataggc accgggcgct
     6961 ggcgcgcgtt gccgtttgct atcaacgtac tgacccacag cagacagcga tatcgacgta
     7021 acttacgctt ctatgctgat gacccaacga ttcgcgtcgg tgggccgacc taccattggg
     7081 tacgcgaaag tattctggct ggcgaacagg tgttagccgg tgcgggtgat gacgccacgc
     7141 caacgcttct cttgcaggct gaagaggaac gcgtggtgga taaccgcatg catgaccgtt
     7201 tttgtgaact ccgcaccgcc gcgggccatc ctgtcgaagg aggacgaccg ttggtaatta
     7261 aaggtgctta ccatgagatc ctttttgaaa aggacgcaat gcgctcagtc gcgctccacg
     7321 ccattgttga ttttttcaac aggcataact cacccagcgg aaaccgctct acagaggttt
     7381 aaatttctta tgtaccaggt tgttgcgtct gatttagatg gcacgttact ttctcccgac
     7441 catacgttat ccccttacgc caaagaaacc ctgaagctgc tcaccgcgcg cggcatcaac
     7501 tttgtgtttg cgaccggtcg tcaccacgtt gatgtggggc aaattcgcga taatctggag
     7561 atcaagtcgt acatgatcac ctccaacggc gcgcgcgttc acgatctgga tggcaatctg
     7621 atttttgctc ataacctgga tcgcgacatt gccagcgatc tgtttggcgt agtcaacgac
     7681 aatctggaca tcattactaa cgtttatcgc gacgacgaat ggtttatgaa tcgccatcgc
     7741 ccggaagaga tgcgcttttt taaagaagcg gtgttcaaat atgcgctgta tgagcctgga
     7801 ttactggagc cggaaggcgt cagcaaagtg ttcttcacct gcgattccca tgaacaactg
     7861 ctgccgctgg agcaggcgat taacgctcgt tggggcgatc gcgtcaacgt tagtttctct
     7921 accttaacct gtctggaagt gatggcgggc ggcgtttcaa aaggccatgc gctggaagcg
     7981 gtggcgaaga aactgggcta cagcctgaag gattgtattg cgtttggtga cgggatgaac
     8041 gatgccgaaa tgctgtcgat ggcggggaaa ggctgcatta tgggcagtgc gcaccagcgt
     8101 ctgaaagacc ttcatcccga gctggaagtg attggtacta atgccgacga cgcggtgccg
     8161 cattatctgc gtaaactcta tttatcgtaa tcgttcttta tttggtcagt tgtcaacctg
     8221 atacttcgct acaatggata ctcgttaatc aaagagtttt ccattgtggc gctacttatc
     8281 atcaccacga ttctgtgggc cttctccttt agcttttatg gcgagtacct tgcggggcac
     8341 gtcgatagct attttgcggt gctggtgcgc gttggcctgg cggcactcgt ttttctgccg
     8401 tttctgcgta cccgtggcaa tagcctgaaa acggtcggcc tgtatatgct ggtgggcgcg
     8461 atgcagctcg gcgtgatgta tatgctgagt ttccgcgctt atctctacct gacggtctcc
     8521 gaattgttgc tgttcaccgt actgacgccg ctctacatca cgctgattta tgacatcatg
     8581 agtaagcgcc gtctgcgctg gggctatgct tttagcgcct tgctggcggt gattggtgcc
     8641 gggattattc gctatgatca ggttaccgac catttctgga ctggcttgtt gctggtgcaa
     8701 ctctccaata tcacttttgc catcggcatg gtgggttaca aacgcctgat ggaaactcgc
     8761 ccgatgccgc agcataacgc ctttgcgtgg ttctatcttg gcgcgttcct ggtggcggta
     8821 attgcatggt tcttgctggg aaatgcgcag aaaatgccgc aaaccacgtt gcaatggggc
     8881 attctggtgt ttcttggcgt ggtggcttcc gggattggct actttatgtg gaactacggc
     8941 gcgacgcagg tggacgccgg aacgctgggc attatgaata atatgcacgt tccggcaggg
     9001 ctgctggtaa acctggctat ctggcaccaa cagccgcact ggccaacgtt cattacaggc
     9061 gcgctggtga tcctggcctc actgtgggtg catcgtaagt gggtcgctcc gcgctcttca
     9121 caaacggcag atgatcgcag gcgtgattgc gcgctgagcg aataaatgct tctgtaatcg
     9181 gctgacgctg ctcgccatcg cgcacggcgg cgtacagtcg gctccacaag ccttcgccca
     9241 gggttttggt caccaccaga ccctggcgct caaaactctc tactacccaa tgcggtagcg
     9301 cggcaatacc catccgcgcg gcaaccatct gaatcaacaa taaggtgtta tcgacgcttt
     9361 tcagtgacgg gctgacgcct gccggctgaa gaaaatgccg ccagacatcc agtcgactac
     9421 gctgcaccgg ataaatcaac agcgtctcgc tggcgagatc ttccggtgta attcgcgttt
     9481 tcgccgccag tggatggtca ggtgctaaca ccagacgcac ttcatagtcg aacatcggcg
     9541 aataatgcag gccactgcgc ggcaggatat cggaggtcat caccagatcc agctcgccct
     9601 gttgcaacgc aggctgcggg tcaaacgtca cgcccgattt aaaatccatc tctacctgcg
     9661 gccagttctt atggaaattt tctaacgcgg gcgtcagcca ctgaatacag ctatggcact
     9721 caatggcaat gcgcagacgc gtctgctgcg gttcattgca ggcttgcagt gcctggctga
     9781 tttgcggcag cacctggttt gccagttgca acaggatttc tccctgcggt gtaaagcgta
     9841 gcggctggct cttacgcaca aatagccgga agccaaggcg ttgttccaga tcgctaaact
     9901 ggtgagacag ggcggattgc gtctgatgca acgtcgccgc agcggctgcg agagagccgc
     9961 agttccgcaa cgcttgtagc gttttcaggt gttttacttc gatcatgaaa gtccttcact
    10021 tcggcatgaa taatttgcgc ttgaggaata tacagtaacc gccaattatg gatgtgtaaa
    10081 catctggacg gctaaaatcc ttcgtctttt aaatttatgg tgcgttggct gcgtttctcc
    10141 accccggtca cttacttcag taagctcccg gggatgaata aacttgccgc cttccctaaa
    10201 ttcaaaatcc ataggattta catataatta gaggaagaaa aatgacaata ttgaatcaca
    10261 ccctcggttt ccctcgcgtt ggcctgcgtc gcgagctgaa aaaagcgcaa gaaagttatt
    10321 gggcggggaa ctccacgcgt gaagaactgc tgacagtagg gcgtgaactg cgtgctcgtc
    10381 actgggatca acaaaagcaa gcgggtatcg acctgctgcc ggtgggcgat tttgcctggt
    10441 acgatcatgt actgaccacc agtttgctgc tgggtaatgt tccgccacgt catcagaaca
    10501 aagacggttc ggtagatatc gacaccctgt tccgtattgg tcgtggacgt gcgccgactg
    10561 gcgaacctgc ggcggcagcg gaaatgacca aatggtttaa caccaactat cactacatgg
    10621 tgccggagtt cgttaaaggc caacagttca aactgacctg gacgcagctg ctggaggaag
    10681 tggacgaggc gctggcgctg ggccacaacg ttaagcctgt gctgctgggg ccggttactt
    10741 acctgtggct ggggaaagtg aaaggtgaac agtttgaccg ccttagcctg ctgaacgaca
    10801 ttctgccggt ttatcagcaa gtgctggcag aactggcgaa acgcggcatc gagtgggtac
    10861 agattgatga acccgcgctg gtactggaac taccacaggc gtggctggac gcatacaaac
    10921 ccgcttacga cgcgctccag ggacaggtga aactgctgct gaccacctat tttgaaggcg
    10981 taacgccaaa tctcgacacg attactgcgc tgcctgttca gggtctgcat gttgatctcg
    11041 tacatggtaa agatgacgtt gctgaactgc acaagcgcct gccttctgac tggttgctgt
    11101 ctgcgggtct gatcaatggt cgtaacgtct ggcgcgccga tcttaccgag aaatatgcgc
    11161 aaattaagga cattgtcggc aaacgtgatt tgtgggtggc atcttcctgc tcgttgctgc
    11221 acagccccat cgacctgagc gtggaaacgc gtcttgatgc agaagtgaaa agctggtttg
    11281 ccttcgccct acaaaaatgc catgaactag cactgctgcg cgatgcgctg aacagtggtg
    11341 acacggcagc tctggcagag tggagcgccc caattcaggc gcgtcgtcac tctactcgtg
    11401 tacataatcc ggcggtagaa aagcgtctgg cggcgatcac cgcccaggac agccagcgtg
    11461 cgaatgtcta tgaagtgcgt gctgaagccc agcgtgcacg ttttaaactg ccagcgtggc
    11521 cgaccaccac gattggttcc ttcccgcaaa ccacggaaat tcgtaccctg cgtctggatt
    11581 tcaaaaaggg caatctcgac gccaataact accgcacggg cattgcggaa catatcaggc
    11641 aggccattgt tgaacaggaa cgtttgggac tggatgtgct ggtacatggc gaggccgagc
    11701 gtaatgacat ggtggaatac tttggcgagc acctcgacgg gtttgtcttt acgcaaaacg
    11761 gttgggtaca gagctacggt tcccgctgcg tgaagccacc gattgttatt ggtgacgtta
    11821 gtcgcccggc accgattacc gtggagtggg cgaagtatgc gcaatctctg actgataaac
    11881 cggtgaaagg gatgctgact ggcccggtga ctattctctg ctggtcgttc ccgcgtgaag
    11941 atgtcagccg tgaaaccatc gccaaacaga ttgcgctggc gctgcgtgat gaagtggccg
    12001 atctggaagc cgctggaatt ggcatcatcc agattgacga accggctctg cgcgaaggtt
    12061 taccgctgcg tcgtagcgac tgggatgcgt atctccagtg gggcgtagag gccttccgta
    12121 tcaacgccgc cgtggcgaaa gatgacacac aaatccacac tcacatgtgt tattgcgagt
    12181 tcaacgacat catggattcg attgcggcac tggacgcaga cgtcatcacc atcgaaacct
    12241 cgcgttctga catggagttg ctggagtcgt tcgaagagtt tgattatccg aatgaaatcg
    12301 gtccaggcgt ctatgacatt cactcgccaa acgtaccgag cgtggaatgg attgaagcct
    12361 tgctgaagaa agcggcaaaa cgcattccgg cagagcgcct gtgggttaac ccggactgtg
    12421 gcctgaaaac gcgcggctgg ccagaaaccc gcgcggcact ggcgaacatg gtgcaggcgg
    12481 cgcagaattt gcgtcgggga tgatgaatat taacgagtca ctgtaaattg cagtacgatg
    12541 aaagaggcaa aactgcctga tactaggcct acataattta tgcaatttat tgaatttttg
    12601 ggttttgttg gccggacaag gcgttcacgc cgcatccggc atgaacaacg cgcactttgc
    12661 caataatctg atggcttcca tcaccggaag ccattttgct atctgcgccc gttcagatac
    12721 agaaataacc gaatcatttt tacgcaaaat ataatcctga gcgatttgtt cagtaatcaa
    12781 cgctacgtta tttattctca cttttcacga aattgtatcc cttttcagta acgtggtagt
    12841 gaggcataga cttatgtccg ctatcctcaa taataaccaa accctcgtta gctaatttgt
    12901 taacaatgtt aacaacatta ccgaatcctg gaatttgcct tatggatagt gtgcgatcga
    12961 gattcatcca gttccagttt atatctttct cagccataat tttcagtacg ttaatttcag
    13021 tttcagttat catagtcaag tgcatcctgg tggtataagt cattaagttc tcccttaagt
    13081 ttataatctt ctagagtagc agtatgcgta t
//
LOCUS       DAESHI010000120        12998 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.121, whole genome shotgun sequence.
ACCESSION   DAESHI010000120 DAESHI010000000
VERSION     DAESHI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.121"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            98..1393
                     /locus_tag="KW011_003467"
     CDS             98..1393
                     /locus_tag="KW011_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBH7661723.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLVLAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="KW011_003468"
     CDS             complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="KW011_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HBH7661724.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2224..3327
                     /gene="asd"
                     /locus_tag="KW011_003469"
     CDS             2224..3327
                     /gene="asd"
                     /locus_tag="KW011_003469"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBH7661725.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3600..5786
                     /gene="glgB"
                     /locus_tag="KW011_003470"
     CDS             3600..5786
                     /gene="glgB"
                     /locus_tag="KW011_003470"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HBH7661726.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5783..7756
                     /gene="glgX"
                     /locus_tag="KW011_003471"
     CDS             5783..7756
                     /gene="glgX"
                     /locus_tag="KW011_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HBH7661727.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7774..9069
                     /gene="glgC"
                     /locus_tag="KW011_003472"
     CDS             7774..9069
                     /gene="glgC"
                     /locus_tag="KW011_003472"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBH7661728.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9069..10502
                     /gene="glgA"
                     /locus_tag="KW011_003473"
     CDS             9069..10502
                     /gene="glgA"
                     /locus_tag="KW011_003473"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBH7661729.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10521..12968
                     /gene="glgP"
                     /locus_tag="KW011_003474"
     CDS             10521..12968
                     /gene="glgP"
                     /locus_tag="KW011_003474"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HBH7661730.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3039 a         3226 c         3576 g         3157 t
ORIGIN      
        1 gtttccacca gtgtcaaaat ctcgacgggg cgtttgttat tacccctcct ttgatgcctg
       61 gtccggcgtt gctggttgac gatatcgtgg attctgcatg gacgctgaca gttctgacag
      121 cactgttacg ccaggcaggt tgcccgacgg tttatcctct tgcccttgct tctacctcgg
      181 taaaaaattg atgaatcttt cagccaatgc acaagcaact ctcctgctaa ccagcgattt
      241 ttctcgcgcg gcggcgagtg agtataaacc tcttagtaat agtgaatggg ggaagtttgc
      301 attatggctg aagcaccaac gtatcagtcc cgccgagctt ctggtgccgc aaccgcaaga
      361 gaaacttaca ggctggagcg atccgcgtat ttctcaggag cgtattcttg gcttgctggc
      421 gcgtggtcat agtctggtgt tggcggtaga taagtggcaa cgcgccggtt tatggatctt
      481 aacccgcgga gatgctgatt atcccgttcg cttgaaaaac cgattgcgaa cggatgcacc
      541 tcccgtttta tttggctgcg ggaataaagc attactgcaa gcggaaggta tggcgattgt
      601 tggctcgcga gatgctccga ctgacgattt gcgctatacc caacaactgg ccgcgaaact
      661 ggcccaacag gggatttgcg ttatctctgg tggtgcgcga ggtattgatg aatgtgcaat
      721 ggcgtcggca ctggaggccg ggggaactgc cgttggcgta ttagctgata gcttgttaaa
      781 aacgagtacg ttagtgaaat ggcgtgaagg gcttatagca ggcaacctgg tgttgatttc
      841 gccgttttac ccagaggtac gtttcaccgt cggcaatgcg atggcgcgaa ataaatatat
      901 ttattgcctt gctgaaagcg caatggttgt acgtgcggga atgaccggtg gaacgataac
      961 cggggcgatg gaggcattaa aacatcagtg gctgcctgtg caggttaaac caaatcagga
     1021 tatgcaatca gccaattcac gattagtaga aaatggggcg tcatggagtg ctgaacaggc
     1081 tgagaatgtg acgatcagac tgccagacgt tcctgggctg atgtatgaca gagcactccg
     1141 taacgctcaa ccagaactgt tttcgctgca tgaagatgac gcaaattacg cagtaatgcc
     1201 cgcgtatacg cctgtcgatt tttatcaact ctttgtggcg gaactggcga tccttgcaaa
     1261 ggaatcgata agtattgaaa ggctggcgtc ttgtactggt ttaaccatcg aacaaattag
     1321 tgtgtggctg aaccgcgcag aagaagaggg aagggttatc cgattgggcg aaggtcatta
     1381 tcagttcagg taataaaaca ggtgccgcgt ctggttgtgt gcagcacctg tttgaacctt
     1441 accccttcat ccacattcga atgccgtcga ggaacatctg ggttgccatc atcaccagaa
     1501 tcaatcccat caggcgttca agtgcgttca cccctttctc gcccagcaga cgtaaaaata
     1561 gcgaagactg tagcaggatg acaaaggtgc cgccccaggc cagcagcaga gcaatcacca
     1621 gatgtcccat ctgattcggg tactgatgag acaacaacat cagcgtggcg agaatagtcg
     1681 gcccggcgac taacggaatt gccaacggca cgataaatgg ctcttcacct gccggaagcc
     1741 cgctgctatt tcctgaagcg ctggggaaaa tcatcttaat ggcgattaga aacagaatga
     1801 tgccgccaga aatggagacg gtttctgctc gcaggctgag aaatgccaga attttctcac
     1861 ccgcaaacag gaacaccagc attaccagga gagcaataag caactctcgc accatgattg
     1921 cccgccgtct tttcggttca gtatgtttca gtacggacat gaaaataggc aggtttccga
     1981 gcggatccat aatcaggatc aataaaactg ctgcagaaat gatttcattc ataactcaaa
     2041 ttccctgata attgccgcgg actttctgcg tgctaacaaa gcaggataag tcgcattact
     2101 gatggcttcg ctatcattga ttaatttcac ttgcgacttt ggctgctttt tgtatggtga
     2161 aagatgtgcc aagaggagac cggcacattt atacagcaca catctttgca ggaaaaaacg
     2221 cttatgaaaa atgttggttt tatcggctgg cgcggtatgg tcggctccgt tctcatgcaa
     2281 cgcatggttg aagagcgcga cttcgacgcc attcgccctg tcttcttttc tacttctcag
     2341 cttggccagg ctgcgccgtc ttttggcgga accactggca cacttcagga tgcctttgat
     2401 ctggaggcgc taaaggccct cgatatcatt gtgacctgtc agggcggcga ttataccaac
     2461 gaaatctatc caaagcttcg tgaaagcgga tggcaaggtt actggattga cgcagcatcg
     2521 tctctgcgca tgaaagatga cgccatcatc attcttgacc ccgtcaatca ggacgtcatt
     2581 accgacggat taaataatgg catcaggact tttgttggcg gtaactgtac cgtaagcctg
     2641 atgttgatgt cgttgggtgg tttattcgcc aatgatcttg ttgattgggt gtccgttgca
     2701 acctaccagg ccgcttccgg cggtggtgcg cgacatatgc gtgagttatt aacccagatg
     2761 ggccatctgt atggccatgt ggcagatgaa ctcgcgaccc cgtcctctgc tattctcgat
     2821 atcgaacgca aagtcacaac cttaacccgt agcggtgagc tgccggtgga taactttggc
     2881 gtgccgctgg cgggtagcct gattccgtgg atcgacaaac agctcgataa cggtcagagc
     2941 cgcgaagagt ggaaagggca ggcggaaacc aacaagatcc tcaacacatc ttccgtaatt
     3001 ccggtagatg gtttatgtgt gcgtgtcggg gcattgcgct gccacagcca ggcattcact
     3061 attaaattga aaaaagatgt gtctattccg accgtcgaag aactgctggc tgcgcacaat
     3121 ccgtgggcga aagtcgttcc gaacgatcgg gaaatcacta tgcgtgagct aaccccagct
     3181 gccgttaccg gcacgctgac cacgccggta ggccgcctgc gtaagctgaa tatgggacca
     3241 gagttcctgt cagcctttac cgtgggcgac cagctgctgt ggggggccgc ggagccgctg
     3301 cgtcggatgc ttcgtcaact ggcgtaatct ttattcatta aatctggggc gcgatgccgc
     3361 ccctgttagt gcgtaataca ggagtaagcg cagatgtttc atgatttacc gggagttaaa
     3421 tagagcattg gctattcttt aagggtggct gaatacatga gtattcacag ccttacctga
     3481 agtgaggacg acgcagagag gatgcacaga gtgctgcgcc gttcaggtca aaaaaatgtc
     3541 acaaccagaa gtcaaaaatc caattggatg gggtgacaca ataaaacagg aagacaagca
     3601 tgtccgatcg tatcgataga gacgtgatta acgcgctaat tgcaggccat tttgcggatc
     3661 ctttttccgt actgggaatg cataaaacca ccgcgggact ggaagtccgt gcccttttac
     3721 ccgacgctac cgatgtgtgg gtgattgaac cgaaaaccgg gcgcaaactc gcaaaactgg
     3781 agtgtctcga ctcacgggga ttctttagcg gcgtcattcc gcgacgtaag aattttttcc
     3841 gctatcagtt ggctgttgtc tggcatggtc agcaaaacct gattgatgat ccttaccgtt
     3901 ttggtccgct aatccaggaa atggatgcct ggctattatc tgaaggtact cacctgcgcc
     3961 cgtatgaaac cttaggcgcg catgcagata ctatggatgg cgtcacaggt acgcgttttt
     4021 ctgtctgggc tccaaacgcc cgtcgggtct cggtggttgg gcaattcaac tactgggacg
     4081 gtcgccgtca cccgatgcgc ctgcgtaaag agagcggcat ttgggaactg tttatccctg
     4141 gggcgcataa cggtcagctc tataaatacg agatgattga tgccaatggc aacttgcgtc
     4201 tgaagtccga cccttatgcc ttcgaagcgc aaatgcgccc ggaaaccgcg tctcttattt
     4261 gcgggctgcc ggaaaaggtt gtacagactg aagagcgcaa aaaagcgaat cagtttgatg
     4321 cgccaatctc tatttatgaa gttcacctgg gctcctggcg tcgccacacc gataacaatt
     4381 tctggttgag ctaccgcgag ctggccgatc aactggtgcc ttatgctaaa tggatgggct
     4441 ttacccacct cgaactactg cccattaacg agcatccctt cgatggcagt tggggttatc
     4501 agccaaccgg cctgtatgca ccaacccgcc gttttggtac tcgcgacgac ttccgttatt
     4561 tcattgatgc cgcacacgca gcgggtctga acgtgattct cgactgggtg ccaggccact
     4621 tcccgaccga tgactttgcg cttgccgaat ttgatggcac gaacttgtat gaacacagcg
     4681 atccgcgcga aggctatcat caggactgga atacgctgat ctacaactat ggtcgccgtg
     4741 aagtcagtaa cttcctcgtc ggtaacgcgc tttactggat tgaacgtttt ggtattgatg
     4801 cgctgcgcgt cgatgcggtg gcgtcaatga tttatcgcga ctacagccgt aaagaggggg
     4861 agtggatccc gaacgaattt ggcgggcgcg agaatcttga agcgattgaa ttcttgcgta
     4921 ataccaaccg tattcttggt gagcaggttt ccggtgcggt gacaatggct gaggagtcta
     4981 ccgatttccc tggcgtttct cgtccgcagg atatgggcgg tctgggcttc tggtacaagt
     5041 ggaacctcgg ctggatgcat gacaccctgg actacatgaa gctcgacccg gtttatcgtc
     5101 agtatcatca cgataaactc accttcggga ttctctacaa ctacactgaa aacttcgtcc
     5161 tgccgttgtc gcatgatgaa gtggtccacg gtaaaaaatc gattctcgac cgcatgccgg
     5221 gcgacgcatg gcagaaattc gctaacctgc gcgcctacta tggctggatg tgggcattcc
     5281 cgggcaagaa actgctgttc atggggaacg aatttgccca aggccgtgag tggaaccatg
     5341 acgccagcct cgactggcat ctgttggaag gcggcgataa ctggcaccac ggtgtccagc
     5401 gtctggtgcg cgatctgaac ctcacctacc gccaccataa agcaatgcat gaactggatt
     5461 ttgacccgta cggctttgaa tggctggtgg tggatgacaa agaacgctcg gtgctgatct
     5521 ttgtgcgtcg cgataaagag ggtaacgaaa ttatcgttgc cagtaacttt acgccggtgc
     5581 cgcgtcatga ttatcgcttc ggcattaatc agccaggcaa atggcgtgaa atcctcaata
     5641 ccgattccat gcactatcac ggcagtaatg caggcaatgg cggcacggta cacagcgatg
     5701 agattgccag ccacggtcgt cagcattcac taagcctgac actaccaccg ctggccacta
     5761 tctggctggt tcgggaggca gaatgacacg actcgccatt ggcaaacccg ctcccctcgg
     5821 cgcgcattac gacggtcagg gcgtcaactt cacacttttc tccgctcatg ccgagcgggt
     5881 agagctgtgt gtctttgacg ccaatggcca ggaacatcgc tatgacttgc cagggcacag
     5941 tggcgacatt tggcacggtt atctgccgga tgcgcgcccg ggtttgcgtt atggttatcg
     6001 cgttcatggc ccctggcaac ccgccgaggg gcatcgcttt aacccggcga agttgttgat
     6061 tgatccttgc gcgcggcaaa ttgaagggga attcaaagat aacccgctgc tgcacgccgg
     6121 tcataatgaa cctgactatc gcgacaacgc cgccattgcg ccgaaatgcg tagtggtggt
     6181 tgatcactat gactgggaag atgatgcccc gccgcgcacg ccgtggggca gcaccatcat
     6241 ttatgaagcc catgttaaag gattaacgta cctgcacccg gagatcccgg tcgagatccg
     6301 tggcacttat aaagccctcg ggcatccggt gatgatcaac tatttgaaac agttgggcat
     6361 taccgcgctg gaactgctgc cggtggcgca gtttgccagt gaaccacgtc tgcaacgcat
     6421 ggggctaagt aactactggg gttacaaccc ggtggcgatg tttgcgctgc atccggcgta
     6481 tgcctgctcg ccagaaacgg cgctgcatga gtttcgcgat gcaatcaaag cactgcataa
     6541 agcgggtatc gaagtcattc ttgatatcgt gctcaaccat agtgcggaac tggacctcga
     6601 cggcccgtta ttctcgctgc gtgggatcga caaccgtagc tattattgga taagagaaga
     6661 cggcgattat cacaactgga ccggttgtgg caacacgctc aatttgagtc atccggcggt
     6721 ggtggattat gcctgcgcct gcctgcgtta ttgggtagaa acctgtcacg tcgatggttt
     6781 ccgctttgat ctggcggcag tcatgggccg tacgccagag ttccgtcagg atgcgccgtt
     6841 gtttaccgct atccagaact gcccggtgct ctcgcaggtg aagttaattg ctgaaccgtg
     6901 ggatatcgcc cttggtggtt atcaggtggg aaatttcccg ccgctgtttg ccgagtggaa
     6961 cgatcatttc cgcgatgctg cccgtcgttt ctggctacat tatgatttgc ctctgggggc
     7021 gtttgccggg cgttttgctg cctccagcga tgtttttaaa cgtaatggtc gtctgccgag
     7081 tgccgcgatt aatctcgtca ctgcgcatga cggttttacg cttcgcgact gcgtttgctt
     7141 caaccataaa cacaatgaag caaacggaga agaaaatcgc gacgggacca acaacaatta
     7201 cagtaacaat catggtaaag aagggttagg cggttctctt gacctggttg aacggcggcg
     7261 cgacagtatt cacgccctgt taacaacgtt gttgctctcc cagggtacgc cgatgttact
     7321 ggccggtgac gaacacggtc acagccagca tggcaataac aatgcctact gtcaggataa
     7381 ccaattaacc tggttggact ggtcgcaggc aagcagtggt ttaaccgcat ttaccgccgc
     7441 gttaatccat ctgcgcaagc gcattcccgc tttggtggag aatcgctggt gggaagaagg
     7501 cgacggcaat gtccgttggc taaatcgata tgctcaacct ttaagcacgg atgagtggca
     7561 aaacgggccg aaacagctgc aaattctgct ctcggatcgc tttttgatcg caattaacgc
     7621 cacgcttgag gtaacagaga ttgttttacc tgctggggag tggcacgcca ttcccccatt
     7681 cgctggagag gataacccag tgattacggc tgtctggcag ggacctgcac acggattgtg
     7741 tgtgttccag agatgataaa aaaggagtta gtcatggtta gtttagagaa gaacgatcac
     7801 ttaatgttgg cgcgccagct gccattgaaa tctgttgccc tgatactggc gggaggacgt
     7861 ggtacccgcc tgaaggattt aaccaataag cgagcaaaac cggccgtaca cttcggcggt
     7921 aagttccgca ttattgactt tgcgctgtct aactgcatca actctgggat ccgtcgtatg
     7981 ggcgtgatca cccagtacca gtcccacact ctggtgcagc acattcagcg cggctggtca
     8041 ttcttcaatg aagaaatgaa cgagtttgtc gatctgctgc cagcacagca gagaatgaaa
     8101 ggggaaaact ggtatcgcgg caccgcagat gcggtcaccc aaaacctcga cattatccgc
     8161 cgttataaag cggaatacgt ggtgatcctg gcgggcgacc atatctacaa gcaagactac
     8221 tcgcgtatgc ttatcgatca cgtcgaaaaa ggcgcacgtt gcaccgttgc ttgtatgcca
     8281 gtaccgattg aagaagcctc cgcatttggc gttatggcgg ttgatgagaa cgataaaatt
     8341 atcgaattcg ttgaaaaacc tgctaaccca ccgtcaatgc cgaacgatcc gagcaaatct
     8401 ctggcgagta tgggtatcta cgtctttgac gccgactatc tgtatgaact gctggaagaa
     8461 gacgatcgtg atgagaactc cagccacgac tttggcaaag atttgattcc caagatcacc
     8521 gaagccggtc tggcctatgc gcacccgttc ccgctctctt gcgtacaatc cgacccggat
     8581 gccgagccgt actggcgcga tgtgggtacg ctggaagctt actggaaagc gaacctcgat
     8641 ctggcctctg tggtgccgga actggatatg tacgatcgca attggccaat tcgcacctac
     8701 aatgaatcat taccgccagc gaaattcgtg caggatcgct ccggtagcca cgggatgacc
     8761 cttaactcac tggtatccgg cggctgtgtg atctccggtt cggtggtggt gcagtccgtt
     8821 ctgttctcgc gcgttcgcgt gaattcattc tgcaacattg attccgccgt attgttaccg
     8881 gaagtatggg taggtcgctc gtgccgtctg cgccgctgcg tcatcgatcg tgcttgtgtt
     8941 attccggaag gcatggtgat tggtgaaaac gcagaggaag atgcacgtcg tttctatcgt
     9001 tcagaagaag gcatcgtgct ggtaacgcgc gaaatgctac ggaagttagg gcataaacag
     9061 gagcgataat gcaggtttta catgtatgtt cagagatgtt cccgctgctt aaaaccggcg
     9121 gtctggctga tgttattggg gcattacccg cagcacaaat cgcagacggc gttgacgctc
     9181 gcgtactgtt gcctgcattt cccgatattc gccgtggcgt gaccgatgcg caggtagtat
     9241 cccgtcgtga taccttcgcc ggacatatca cgctgttgtt cggtcattac aacggggttg
     9301 gcatatacct gattgacgcg ccgcatctct atgatcgtcc gggaagcccg tatcacgata
     9361 ccaacttatt tgcctatacc gacaacgtat tgcgttttgc gcttctgggg tgggttgggg
     9421 cagaaatggc cagcgggctt gacccattct ggcgtcctga tgtggtgcat gcgcacgact
     9481 ggcatgcagg ccttgcgcct gcgtatctgg cggcgcgcgg gcgtcctgcg aagtcggtgt
     9541 ttactgtgca caacctggcc tatcaaggca tgttttatgc acatcacatg aatgacatcc
     9601 aattgccatg gtcattcttt aatattcatg ggctggaatt caacggacaa atctctttcc
     9661 tgaaggccgg tctgtactat gccgatcaca ttacggcggt cagtccaacc tacgctcgcg
     9721 agatcaccga accgcagttt gcctacggta tggaaggtct gttgcaacag cgtcatcgtg
     9781 aagggcgtct ttccggcgta ctgaacggcg tggacgagaa aatctggagt ccagagacgg
     9841 acttactgtt ggcctcgcgc tacacccgcg atacgttgga agataaagcg gaaaataagc
     9901 gccagttaca aatcgcaatg gggcttaagg ttgacgataa agtgccgctt tttgcagtgg
     9961 tgagccgtct gaccagccag aaaggtctcg acctggtgct ggaagcctta ccgggtcttc
    10021 tggagcaggg cgggcagctg gcgctactcg gcgcgggcga tccggtgctg caggaaggtt
    10081 tccttgcggc ggcagcggaa taccccggtc aggtgggcgt tcagattggc tatcacgaag
    10141 cattttcgca tcgcattatg ggcggcgcgg acgtcattct ggtgcccagc cgttttgaac
    10201 cgtgcggctt aacgcaactt tatggattga agtacggtac gctgccgtta gtgcggcgca
    10261 ccggtgggct tgctgatacg gtttctgact gttctcttga gaaccttgca gatggcgtcg
    10321 ccagtgggtt tgtctttgaa gataataatg cctggtcgct gttacgggct attcggcgtg
    10381 cttttgtact gtggtcccgt ccttcactgt ggcggtttgt gcaacgtcag gctatggcaa
    10441 tggattttag ctggcaggtc gcggcgaagt cgtaccgtga gctttactat cgcttgaaat
    10501 agttttcagg aaacgcctat atgaatgctc cgtttacata ttcatcgccc acgcttagcg
    10561 tagaagctct taagcactct atcgcttaca agctgatgtt tacgattgga aaggacccgg
    10621 tcgtcgccaa taaacatgaa tggctgaacg caacgttatt tgctgtgcgc gatcgtctcg
    10681 tggagcgctg gttacgttca aaccgtgccc agttgtcgca agaaacacgt caggtttact
    10741 acctgtcgat ggagtttttg attggccgta cgctctccaa cgccatgttg tcgctaggaa
    10801 tttacgaaga tgtacagggc gcactggaag cgatggggtt aaatctcgaa gagctgattg
    10861 atgaagaaaa tgacccaggc ctcggtaacg gtggcctggg acgtctggcg gcttgcttcc
    10921 ttgattctct ggcgacgtta gggttgccgg ggcgcggtta cggcatccgc tatgactacg
    10981 gtatgttcaa gcagaacatc gttaacggta gccagaaaga gtcgccagac tactggctgg
    11041 aatacggtaa cccgtgggaa ttcaaacgcc acaacacgcg ctataaagtc cgttttggcg
    11101 gtcgcattca gcaggaaggt aaaaaaacgc gctggattga aaccgaagag attctgggag
    11161 tcgcttacga tcagataatc cctggttacg acaccgacgc gaccaacacg ctgcgtttgt
    11221 ggagtgcgca agccagtagc gaaattaacc tcggtaaatt caaccagggt gactacttcg
    11281 cggcagtgga agataaaaac cactccgaga acgtatctcg cgtactgtat ccggatgact
    11341 ccacctactc cgggcgtgag ctgcgcctgc gtcaggaata cttcctggtt tcctcgacca
    11401 ttcaggacat tttaagccgc cattatcagt tgcataaaac ctacgataac ctggcggata
    11461 aaatcgcgat tcatctcaat gatacccatc cggtactgtc gattcctgag atgatgcgtc
    11521 tgctgatcga tgagcaccaa tttagctggg acgacgcgtt tgaggtgtgt tgtcaggtct
    11581 tctcctacac taaccacacg ctgatgagcg aggcgctgga aacctggccg gttgatatgc
    11641 tgggtaaaat tctgccgcgt cacctgcaga tcatctttga aatcaacgac tatttcctga
    11701 aaaccttgca ggaacagtat ccgaacgata ccgatctgct gggacgggcg tcgatcattg
    11761 atgaatccaa cggtcgtcgt gtgcgtatgg cctggctggc ggttgttgtg agccacaaag
    11821 ttaacggtgt atcggaactg cactctaatc tgatggtgca atcgttgttt gccgactttg
    11881 cgaaaatctt cccgggtcgt ttcaccaacg tcaccaacgg tgtgacgccg cgtcgctggc
    11941 tggcggtagc gaacccatcg ctttcagccg tgctggacga acacctgggc cgtaactggc
    12001 gcaccgacct tagcctgctt aatgagctgc aacaacactg tgatttccca atggttaatc
    12061 acgctgtgca tcaggcgaag ctggagaaca aaaagcgtct ggcagagtat atcgcccagc
    12121 agctgaatgt ggtggtgaat ccaaaggcgt tgttcgatgt acaaatcaaa cgtattcacg
    12181 aatacaaacg tcaattgatg aatgtgttgc atgtgattac ccgctataac cgcatcaagg
    12241 ccgacccgga tgcgaagtgg gtaccgcgcg tgaatatttt tggcggtaag gcggcttcgg
    12301 cctattacat ggcgaagcac attattcatt tgatcaatga cgtagcgaaa gtgatcaaca
    12361 acgatccgca gattggcgat aagctgaaag tcgtgttcat cccgaactac agcgttagcc
    12421 tggcgcagtt gatcattccg gcggcagatc tgtctgaaca gatttcgctg gcagggacgg
    12481 aagcttccgg caccagtaac atgaagtttg cgcttaacgg tgcgctgact atcggtacgt
    12541 tggacggtgc gaatgtcgag atgctggatc atgtcggtgc tgacaatatc tttatttttg
    12601 gtaacacagc ggaagaagtg gaagaactgc gtcgtcaggg ctacaaaccg cgtgaatact
    12661 acgagaaaga tgaggagctg catcaggtgc tgacgcaaat cggcagcggt gtattcagtc
    12721 cggaagatcc gggtcgctat cgcgatctgg ttgattcgct gatcaacttc ggcgatcact
    12781 atcaggttct ggcggattat cgcagctatg tcgattgtca ggataaagtc gacgaactct
    12841 acgagcttca ggaagagtgg accgcaaaag cgatgctgaa cattgccaat atgggctact
    12901 tctcttctga ccgtactatc aaagagtacg ccgatcatat ctggcatatc gatccggtga
    12961 gattgtaagt tcaccaataa atagaacggg gccaaagg
//
LOCUS       DAESHI010000121        12563 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.122, whole genome shotgun sequence.
ACCESSION   DAESHI010000121 DAESHI010000000
VERSION     DAESHI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12563
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.122"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..833)
                     /gene="gap"
                     /locus_tag="KW011_003475"
     CDS             complement(<1..833)
                     /gene="gap"
                     /locus_tag="KW011_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBH7661731.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IV"
     gene            1022..1552
                     /gene="cybB"
                     /locus_tag="KW011_003476"
     CDS             1022..1552
                     /gene="cybB"
                     /locus_tag="KW011_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HBH7661732.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1797..1970
                     /locus_tag="KW011_003477"
     CDS             1797..1970
                     /locus_tag="KW011_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661733.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(2042..2191)
                     /gene="hokB"
                     /locus_tag="KW011_003478"
     CDS             complement(2042..2191)
                     /gene="hokB"
                     /locus_tag="KW011_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HBH7661734.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            2590..4230
                     /gene="trg"
                     /locus_tag="KW011_003479"
     CDS             2590..4230
                     /gene="trg"
                     /locus_tag="KW011_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HBH7661735.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(4268..5191)
                     /locus_tag="KW011_003480"
     CDS             complement(4268..5191)
                     /locus_tag="KW011_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH7661736.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            5408..6709
                     /locus_tag="KW011_003481"
     CDS             5408..6709
                     /locus_tag="KW011_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH7661737.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLY"
     gene            6976..8631
                     /gene="opgD"
                     /locus_tag="KW011_003482"
                     /gene_synonym="mdoD"
     CDS             6976..8631
                     /gene="opgD"
                     /locus_tag="KW011_003482"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HBH7661738.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            8771..8995
                     /locus_tag="KW011_003483"
     CDS             8771..8995
                     /locus_tag="KW011_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HBH7661739.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            9058..9594
                     /gene="rimL"
                     /locus_tag="KW011_003484"
     CDS             9058..9594
                     /gene="rimL"
                     /locus_tag="KW011_003484"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HBH7661740.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(9589..10569)
                     /locus_tag="KW011_003485"
     CDS             complement(9589..10569)
                     /locus_tag="KW011_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HBH7661741.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            10693..11685
                     /gene="tehA"
                     /locus_tag="KW011_003486"
     CDS             10693..11685
                     /gene="tehA"
                     /locus_tag="KW011_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HBH7661742.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            11682..12275
                     /gene="tehB"
                     /locus_tag="KW011_003487"
     CDS             11682..12275
                     /gene="tehB"
                     /locus_tag="KW011_003487"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HBH7661743.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         3208 a         2927 c         3146 g         3282 t
ORIGIN      
        1 actatttctt catcggtata accaaatgac tcgttattgg tggttgcttg tttaagtgcg
       61 ttattcacct cttcggcagt cacttttttt ccgagaatcg ataccagttc agtgaccgaa
      121 cctgttttca ccggcacgcg ttgcgcatga cctttcagtt tgccgctcag ttccgggatc
      181 accagaccaa tggcttttgc cgcccccgta gtgtggggaa tgatattttc tgccgctgcg
      241 cgtgaagcac gtaaatcttt accacgtggg ccatccacca gtgactgggt gccagtatag
      301 gcatgaatgg tcgtcatcgt gccgacttct atcccgaaac tgtcatgcaa ggctttggcc
      361 atcggcgcaa gacagttagt ggtgcatgac gccacggaaa caatggtgtc gttgccatcc
      421 agagtgtcgt cattgacgtt ataaacgata gttttcattt caccggcagg ggcggaaatc
      481 aacaccttct tcgcaccagc atcaagatgt gcctgcgatt tctcggcgga ggtataaaag
      541 ccagtacatt cgacaatgat ttctgcacct ttcgctttcc acggaatatt tttagcctct
      601 ttttcggcgt aaaccgcgat acttttccca tcaacgataa gtgaatcttc cgtaaaatca
      661 acgctccagg ggaatggtcc gtagtttgaa tcatgtttca gcaggtaggc gagaattttt
      721 ggggaagtga gatcattaat agcgacaacg tctatgttgc ttttgacttc aagtaatcga
      781 cgcaacacca gtcgaccgat acgaccaaaa ccgttaatac caactttact catggttttc
      841 tcctgtcagg aacattcgga tgaaaattga tcctttccag gcttagacca ggattgtggg
      901 atgggcaatc tccattctta cagtgaaacg taacgtactg aaaacgggtg aacaatattt
      961 aatgaaattt tgagaaaagc ccgttatgtt aacggaaaat tatgttaaag caggaaatgt
     1021 tatggaaaat aaatactcaa ggttacaaat cagcattcac tggctggtct ttttgctggt
     1081 tatcgcagcg tattgcgcaa tggagtttcg tggtttcttc ccacgtagcg atcggccact
     1141 catcaacatg attcatgttt cctgcggcat ctcaattctc gtgctgatgg tcgttcgtct
     1201 tttgttaagg ctgaaatacc caaccccgcc gattatacct aagccaaaac cgatgatgac
     1261 gggactggcg catttgggac atttggtgat gtatctcctg tttattgcgc tgccagtgat
     1321 tggtttggtg atgatgtata accggggcaa cccgtggttt gcgtttggtt tgacgatgcc
     1381 ttacgcttca gaggccaatt tcgaacgggt tgatagctta aagtcgtggc atgagacgct
     1441 ggcgaatctg ggatattttg tcatcgggtt gcacgctgcg gctgcactgg cacaccacta
     1501 tttctggaag gacaacacac ttctacgcat gatgccgcgt aaacgttcct gaaggatatt
     1561 tcaagaaaat gcctgtacta aaaccgaccc gtggtacagg cgaagaatac gggtctacat
     1621 cggaagcgcc tattatattt atttgtatga caaataaaac cataatcctt gcccatacgt
     1681 ccatctggct tatttttaat caattcaccc gatctttgat ctcatcaacg gtatcaaaat
     1741 aaaccagcgt ataacgttca aatcgaacat aagattcgat aaaccatgga ggttatatga
     1801 aaaaactggc acttattttg tttatgggaa cgcttgtttc cttttatgcc gatgccgggc
     1861 gcaaaccctg ttctggttcg aaagggggga tctcacactg tacggcaggc ggcaaatttg
     1921 tctgtaatga tggttctatt agtgcatcga aaaaaacatg cactaactga agtgtaaaag
     1981 gggtgccatg agaaaatgaa tctgctagtc agatgcgcgg ggaaaatccc cgcgcttgcc
     2041 cttacctgga cgtgcaggcc atgagcgcag caacctcctt atcaccgtcc cggaaccgca
     2101 gttcgtagag cgtttgtcgg gtcaggagtg tgaatgtcag aatcgtaatg cagataatga
     2161 gcagacacac taccagaggg ttgtgcttca tagcctttct ccttgccgga tggcgggtaa
     2221 gaggctaaga tctgaattgc taggttcatt cgttggcctc ggttgataga aatatcggtc
     2281 ggggccttcg tctttctgat tcccggttag cctgaaaaca gaaagtctca ggcacccgca
     2341 ggcatcctat gaggtttcct tagggacgaa aataatcact tcacgaaatt gcgtgctgtt
     2401 ttccagaatt tttcgtcatt cgggttagcc agtttagcca ttcgttactc tcttcattcc
     2461 aatagcatta attttctctg caataattgt tgtaaaaatg tgacgcaaag aggtttttgg
     2521 tcataagtaa ttaccgtcaa gtgccgatga ctttctatca ggagtaaacc tggacgagag
     2581 acaacggtaa tgaatacaac tccctcacag cgattaggtt ttttgcatca catcaggctg
     2641 gttccgttat ttgcctgcat tcttggcggt atcttagttc tattcgcatt aagttctgct
     2701 ctggctggct atttcctctg gcaggccgat cgcgatcagc gtgatgttac tgcggagatt
     2761 gagattcgga ccgggttagc gaacagttca gattttttgc gttcagcccg gatcaatatg
     2821 attcaggccg gggctgcgag ttgtattgcg gaaatggaag caatgaagcg aaatattgcg
     2881 caagccgaat cggagattaa acagtcgcag caagggtatc gtgcttatca gaatcggccg
     2941 gtgaaaacac ctgctgatga agccctcgac actgaattaa atcaacgctt tcaggcttat
     3001 atcacgggta tgcaacctat gttgaaatat gccaaaaatg gcatgtttga agcgattatc
     3061 aatcatgaaa gtgagcagat ccgaccgctg gataatgctt ataccgatat tttgaacaaa
     3121 gccgttaaga tacgtagcac cagagccaac caactggcgg aactggccca tcagcgcacc
     3181 cgcttgggtg ggatgttcat gattggcgcg tttgtgcttg ccctggtcat gacgctgata
     3241 acatttatgg tgctacgtcg gatcgtcatt cgtccactgc aacatgccgc acaacggatt
     3301 gaaaaaatcg ctagtggcga tctgacgatg aaggatgaac cggcgggtcg taatgaaatc
     3361 ggtcgcttaa atcgtcattt acagcaaatg cagcattcac tggggatgac agtagggact
     3421 gttcgacagg gtgcggaaga gatttatcgt ggcaccagcg aaatttcagc tggcaatgcg
     3481 gacctgtcat ctcgcaccga agaacaagcg gcggctatcg aacaaactgc cgccagcatg
     3541 gagcaactca ctgcgacggt gaaacagaat gcggataacg cgcatcatgc cagcaaactg
     3601 gcgcaagagg cttctattaa agccagcgat ggcgggcaga cggtttccgg tgtagtaaaa
     3661 acgatgggcg ctatctccac gagttcgaag aaaatttctg agatcaccgc cgtcatcaac
     3721 agtattgctt tccagacgaa tattctggca ctgaatgctg ccgttgaagc cgcgcgagcg
     3781 ggtgagcaag ggcgtggatt tgccgttgtc gccagcgaag tacggacact cgcaagtcgc
     3841 agcgctcagg cggcgaaaga gattgaaggc ttgatcagtg aatcagtcag gttaattgac
     3901 ctggggtcgg atgaggtggc aacggccggg aaaaccatga gcactattgt tgatgccgtc
     3961 gcgagtgtca cacatatcat gcaggaaatc gccgccgcct cggatgaaca aagtagaggc
     4021 ataacgcagg ttagccaggc gatttctgaa atggataagg tgacgcaaca gaatgcttct
     4081 ctggtagaag aggcctcagc ggcggcggtg tcccttgaag aacaggcggc acgattaact
     4141 gaggcggtgg atgtattccg tctgcacaaa cattctgtgt cggcagaacc tcgcggagcg
     4201 ggtgaaccag ttagtttcgc tacggtgtga aaatgttcaa ggagggatcg acagatccct
     4261 tcacctttca gaacggcatt gattttcgaa tagcgttaat catcaactgg caaccagaag
     4321 agaacgtcgc atctacgcgg gtcagtattc caatcggttc gcctgcacca tgtcccggaa
     4381 caggcagggc caccagcgtg gcatgacgca ggtcgtcttt tacagcgcca gaagggacaa
     4441 accacacgta atcgtattca accgtaagtt gacgagatag cgaagcagac agcgtttcga
     4501 tacaacccga aggaatttta cagccctggc tctgcactaa tgcatctgaa tgctggcgtg
     4561 gcgcagtgcc ttctggtgat acaacgaccg gccattccag cacccggctt agcgttacgt
     4621 tctcctgaag tagcgggtga ttagggcgga caaccagctt cagcgattca agaaacagca
     4681 gttcgtaatt aagcccggtc atcagttcag gatctgacat ccgaccaatg ccgatatcga
     4741 tttccccggt ttttaaaccc gccagaatca tagggttact cattgtcgca acttgcaagg
     4801 tcgtctcttt ttgttgctga tgaaactgac ctataaccga aggtaatatc cccagtgccg
     4861 cagtaggtag tgcaccaacc ctgacgacat cattattaag accttcttta cgatgaagcg
     4921 actgtccggc agtgttgatg gcgtcaagaa ctctgactgc atgcgttaaa aattgttcgc
     4981 cgggtaaggt aagttgcgcc ccctgacgac cacgctcaaa caagcgagcg cccgtcagct
     5041 gctccagttc attcaatgtc ttagagagcg caggttgact caaattaagg gtttcagccg
     5101 cgcgccccaa agttccttgt tgtgcgacag ctacgaatgt atgaaggtgg cgcaaacgga
     5161 tgcgctgact aaacagacta tttttttcca taagcgatgt taaaaacgaa gcggtgtcgc
     5221 tgacaagtga agttgtttga ttatgataac ttgattgcaa aatattatta acaattaaag
     5281 caattatgtt acagcaaaat ggataatatt gatgttttcg cggcgagatc acagtttgta
     5341 aattcttccc gccagagtga atgcggttac ctacactcca gattactgac cactggaggc
     5401 agacactatg gcgaacagca tcacggcgga tgagattcgg gaacagtttt cgcaggcaat
     5461 gtcagccatg taccagcaag aagttccgca atatggcacg ctgctggaac tggtagctga
     5521 tgtgaatctg gctgtgctgg aaaacaatcc tcaactgcac gaaaaaatgg taaatgcaga
     5581 cgagctggcg cgactgaatg ttgaacgtca tggggcgatt cgcgttggga ctgcacaaga
     5641 gcttgctact cttcggcgga tgtttgccat tatggggatg tacccggtga gctattacga
     5701 tctctcgcag gcaggggtgc cggtacattc gacagcattt cggcccattg atgatgcttc
     5761 tctggcgcgt aatcccttcc gcgtttttac ctccttactc cgccttgagc ttatcgagaa
     5821 cgaaattttg cgccagaaag cggcggagat tctgcgtcag cgcgatatct tcaccccacg
     5881 ttgtcgacaa ctgttagagg aatatgagca gcagggcggt tttaacgaaa cacaggcaca
     5941 ggagtttgtg caggaagccc tggaaacgtt tcgctggcac cagtcagcaa cggtagatga
     6001 agaaacctat cgcgccttgc acaacgaaca tcggttgatt gctgatgtgg tctgttttcc
     6061 tggatgccat atcaaccacc tgacgccacg tacgctggat attgaccggg tgcagtcgat
     6121 gatgcctgaa tgcggaattg aacccaaaat tctcatcgag gggccgccgc gccgcgaggt
     6181 accgatttta ctacgccaga ccagctttaa agcactggaa gagacggtgt tgtttgcggg
     6241 gcagaaacag ggcacgcata ccgcgcgctt tggtgaaatt gagcagcgtg gcgtggcatt
     6301 aacgccgaaa gggcgacaac tgtatgatga tcttctgcgt aacgctggaa ccgggcagga
     6361 taatctcact caccaaatgc atttacagga aaccttccgc acttttcctg acagtgagtt
     6421 tttaatgcgt cagcaagggt tggcatggtt ccggtaccgt ctgacgcctt cgggtgaggc
     6481 gcatcgtcag gcgattcatc ctggagacga tccacagccc ttaattgaac gtggttgggt
     6541 agtggcgcaa cccatcacct atgaagattt cttgcccgtt agcgcggcgg ggatcttcca
     6601 gtcaaatctg ggtaatgaaa cgcagacacg cagtcacggt aatgccagtc gcgaagcatt
     6661 tgagcaggcg ttgggttgtc cggttttgga tgagttccag ctttactagg aagcggaaga
     6721 acgcagtaaa cgtcgctgtg gtttgcttta aattctgacc atccgccttt gcaaaaattt
     6781 gcctgatttt acaaacgaat caggctcatc ccatcgacat aaaaaaaatg ccgatttatg
     6841 catattctct cagttcaaca attggattat taataaatat tgtctagagt gagcggtcat
     6901 aaataagcac tttcttgccg ctgaaaacga ccagcgcggg accattcaca acaccagaag
     6961 gactcacttt caggtatgga tcgtagacga tttattaaag gttcaatggc tatggccgcc
     7021 gtgtgcggta ccagcggcat tgcttctctt ttttctcagg cggcattcgc ggcagattct
     7081 gatattgccg acgggcaaac ccagcgtttt gacttctcca ttctacagtc aatggcgcac
     7141 gacttagcgc aaacagcgtg gcgtggtgcg cctcgtccgt tacctgacac gctggcgaca
     7201 atgacgccgc aggcttataa cagtattcaa tacgacgccg aaaaatcgct ctggcataac
     7261 gttgagaacc gtcaactgga cgctcagttc ttccatatgg gaatgggatt ccgtcgccgc
     7321 gttcgtatgt tttctgtaga tccagcaaca catctggcgc gtgaaattca ctttcgcccg
     7381 gagttgttca aatacaacga tgcaggtgtt gataccaaac aattagaagg gcaaagcgat
     7441 ctcggctttg ccggttttcg cgtgtttaaa gcccccgaac tggcgcgccg tgatgtagta
     7501 tcatttctcg gcgcgagtta tttccgcgcc gttgatgata catatcaata cggtttgtcg
     7561 gcccgcggcc tggcgatcga cacttacacc gacagtaaag aagagttccc cgactttacc
     7621 gccttctggt ttgatacggt aaaaccgggg gcaactacct ttaccgttta tgcgttgctc
     7681 gatagcgcca gtattactgg tgcctataag ttcactatcc attgtgagaa aagtcaggtg
     7741 attatggatg tggaaaatca cctgtatgcg cgcaaagaca ttaaacagct gggcattgcg
     7801 ccgatgacca gtatgttcag ctgcggtact aatgaacgtc ggatgtgcga caccattcat
     7861 ccgcaaattc atgactctga tcgtctgtcc atgtggcggg gcaacggcga gtggatttgc
     7921 cgtccgctga acaatccgca aaaattgcag ttcaatgctt acaccgacaa caacccgaaa
     7981 gggtttggtt tattgcaact ggatcgtgac ttctcccatt atcaggacat tatgggctgg
     8041 tataacaaac gcccaagttt gtgggtggaa ccgcgtaaca agtggggtaa gggcaccatc
     8101 ggcctgatgg aaatcccaac aacgggcgaa acgctggata acattgtctg cttctggcag
     8161 ccagaaaaag ctgtaaaagc aggtgatgag tttgcattcc agtatcgtct gtactggagt
     8221 gcgcaaccgc ctgttcattg cccattagcg cgcgttatgg cgacgcgtac cggcatgggc
     8281 ggtttcccgg aaggttgggc gccaggtgaa cactatcccg aaaaatgggc gcgtcgtttt
     8341 gccgtcgatt tcgttggtgg tgatctgaaa gctgccgcgc caaaaggcat tgagccggtg
     8401 attacgcttt ccagtgggga agcgaagcaa atcgaaattc tctatattga acccatcgat
     8461 ggttatcgta ttcagtttga ctggtatccg acttcggact ctactgatcc ggtcgatatg
     8521 cggatgtatc tacgttgtca gggggacgct atcagtgaaa catggctgta tcagtatttc
     8581 ccgccagcgc cggataaacg tcagtatgtt gacgaccgcg tgatgagtta atcgtttttt
     8641 cttcggcacc ttcttcggga ggtgccgtct ggttaaacac gatcccgctc gcatttttcc
     8701 ctaagttaaa tgagtaatct gatggtgtgt atttcagata caccttgtca gccactaaca
     8761 gggagtgcgt atgtttccag aataccgaga tttaatatcc cgtctgaaaa acgaaaatcc
     8821 tcgctttatg tccttgttcg ataaacacaa taaacttgat catgaaattg ccagaaagga
     8881 aggttccgac ggtcgagggt acaatgcgga agtggtccgc atgaaaaaac aaaagctaca
     8941 gttaaaagat gagatgctca aaatcctgca gcaggagagc gtcaaagagg tgtaaacttt
     9001 cctaagccgc cagccaggcg gcttttttaa caacagcatg gattgactgg agataagatg
     9061 actgaaacga taaaagtaag cgaatcactt gaattacatg ctgttgcgga aagtcacgtc
     9121 acacctcttt atcagttaat ctgtaaaaat aaaacctggt tacagcagtc gctaaactgg
     9181 ccgcagttcg ttcagagtga agaggacacg cgaaaaacgg tgcagggtaa tgtgatgttg
     9241 catcaacgcg gctatgccaa aatgttcatg attttcaaag aagatgaact tatcggcgtt
     9301 atctcgttta atcgtattga accactgaat aaaactgctg aaatcggcta ctggctggac
     9361 gaatctcatc aggggcaggg gatcatttct caggcgctgc aggcattgat tcatcattac
     9421 gctcagtctg gtgaacttag acgcttcgtg atcaaatgtc gggtggacaa tccgcaaagc
     9481 aaccaggtcg ctttgcgcaa tggttttatc cttgaaggtt gcctgaaaca ggctgagttc
     9541 ctgaatgatg cctatgatga tgtgaactta tacgcgcgta ttatcgattc ataataaccc
     9601 tgccagcggc gttcgcgtaa tgcgctcctc gccgttaatg acttttggac cacggacatg
     9661 aaccgtgtcg ccatcaaagg cttcaacgac ggcgtggtcg gttaactcca catggttttc
     9721 aattatcacc gcacctgaaa tacggctctc accttgtatt acaacgtgct cgtcgagcag
     9781 aatcggtcca ccacgtacca cggcatttcc gccaatcaag acatgatgtt ttaacacaca
     9841 attaccttcc acaatggcat attccgccac ctgcgaactg taatgaatcg tcggaatggc
     9901 atcttcttct atgccagctt tcacctgcgc gtgaccgtag actttagcgc aatcgcatag
     9961 ccagacgttg ttctcttcat taccttcaac actggcaaaa tcaaaaactt cggcgcgatg
    10021 ttcaataaaa gcataccgga cgacggcatc gccataaatt tgtgcctggt ggacaatacg
    10081 cgaggcgcta acccttgcgc gatcataaat ttggagcagg agttgatggt cgggcgttaa
    10141 gccttgtgcg gcgacgatca tagaatgttg atcaattaag gcgtgaccaa atattctaca
    10201 ttgcccgtaa actaatgagt cacgaatggt gacactgtca cttatatatg caccctggct
    10261 gatttcacta ttatcgatcc agacgttatc cgttgcgtaa acctctcccc acagcacgct
    10321 ggtgccggtg atacgcgtat taccagaaat caccgttccg ccgaacgcaa tagcattctg
    10381 gtcgtaaatc cagcaatttc cttcctgtgc taacaccgtt tcgcgatcga tccagccgcc
    10441 agcggttcct gctatcacat cgttaaaatc gctaatggcg ataatctgcc gtaataacac
    10501 acttttttta gtgccatctt cctgataact aaaggctcgc tgttcttcac ttagacgata
    10561 tttgcgcata gcgttttctc acaggtgact tactataacc gtagcaaatt ctgcggctct
    10621 ggctatgctc gagaaaattc cataaaatgc atttcaaata tactttataa attaaacaaa
    10681 atgagtaaga agatgcagag cgataaagtg ctcaatttgc cggcaggcta ctttggtatt
    10741 gtgttgggga cgatagggat gggatttgcc tggcgctatg ccagccaggt ttggcaggtc
    10801 agccactggt taggggatgg gctggtgatt ctggcgatga tcatctgggg attattaact
    10861 agcgcattta ttacccgact catacgcttt ccgcatagcg tgctggcgga agttcgccat
    10921 ccagtgctga gcagttttgt gagtttgttt ccggcaacga cgatgctggt ggcgattggt
    10981 tttgttccgt ggtttcgccc actggcggtg tgcctgttca gttttggtgt cgtggttcag
    11041 ttggcttatg ccgcctggca aactgcggga ttatggcgcg gatctcaccc tgaagaagct
    11101 accacgcctg gactgtatct gccgacagtt gccaacaact ttatcagcgc aatggcctgt
    11161 ggtgcgttgg gctacaccga cgccggtctg gtgtttttag gcgcaggcgt tttctcatgg
    11221 ctaagtcttg aaccggtgat cttgcagcgt ctgcgcagtt cgggagaatt acccacggca
    11281 ctgcggacat cactcggcat tcagctcgct cctgcgctgg tggcctgtag tgcctggttg
    11341 agcgtcaacg gcggcgaggg tgatacgctg gcgaaaatgc tttttggtta tggactgctg
    11401 caactgctgt ttatgctacg tctgatgcca tggtatctct cccagccatt taatgcttca
    11461 ttctggagtt tctcgttcgg cgtatctgca ctggcaacca ccggtttgca tctggggagt
    11521 ggcagcgaaa atggattttt ccatacgctg gcggtaccgc tgtttatctt taccaatttt
    11581 attattgcaa tactgctcat ccgtactttt gcgcttctga tgcagggaaa attgttagtc
    11641 agaaccgagc gcgccgtttt aatgaaagca gaggacaaag aatgatcatt cgtgacgaaa
    11701 actattttac tgataaatat gaattaaccc gcacacactc tgaagtactg gaagcggtga
    11761 aagtggttaa accgggtaaa acgctggatc tgggctgtgg caatggtcgt aacagtcttt
    11821 acctggcagc caatggttat gatgttgacg catgggataa aaatgccatg agtatcgcca
    11881 atgtagagcg cattaaatcc attgaaaatc tggataattt acacacccga gtcgttgatc
    11941 tgaataacct cacatttgat agacagtacg attttattct ttcgactgtg gtactgatgt
    12001 tccttgaggc taaaaccatc ccagggctga ttgccaatat gcaacgttgc actaaacctg
    12061 gtggttacaa cctgattgtg gcggcgatgg ataccgctga ttatccatgt accgtcggct
    12121 tcccgtttgc cttcaaagag ggagaattac gtcgatatta cgaaggctgg gagagggtga
    12181 aatacaatga agacgtcggc gagctgcacc gcaccgacgc caacggtaat cgtattaaac
    12241 tgcgtttcgc cacgatgctg gcacgtaaaa aatgacccgc taagaataag gcgcgtgaaa
    12301 attccccacg ctgagatgat ttactgttct tcttttcggt aagcatattt tttatcgaag
    12361 ggatgtgaaa ttaatcacag tagtcgaagt tttttagcag cttaacttac tgaaatttaa
    12421 ctactgatga ttgacttagc ccctttttcg gcattgacta tgtcgtctga aaaggggctg
    12481 aaaaatttat tttcaccaac actttttttg ccacaacacg aagcggcgct ttttgctata
    12541 acttaaaaag taatataatc atc
//
LOCUS       DAESHI010000122        12221 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.123, whole genome shotgun sequence.
ACCESSION   DAESHI010000122 DAESHI010000000
VERSION     DAESHI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12221
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.123"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..510
                     /gene="csgD"
                     /locus_tag="KW011_003488"
     CDS             <1..510
                     /gene="csgD"
                     /locus_tag="KW011_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HBH7661744.1"
                     /translation="DDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPE
                     DYPYRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYN
                     STESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAV
                     SWANDNLRR"
     gene            515..904
                     /gene="csgE"
                     /locus_tag="KW011_003489"
     CDS             515..904
                     /gene="csgE"
                     /locus_tag="KW011_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HBH7661745.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            929..1345
                     /gene="csgF"
                     /locus_tag="KW011_003490"
     CDS             929..1345
                     /gene="csgF"
                     /locus_tag="KW011_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HBH7661746.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1372..2205
                     /gene="csgG"
                     /locus_tag="KW011_003491"
     CDS             1372..2205
                     /gene="csgG"
                     /locus_tag="KW011_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HBH7661747.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2270..2761)
                     /locus_tag="KW011_003492"
     CDS             complement(2270..2761)
                     /locus_tag="KW011_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBH7661748.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(2863..3417)
                     /gene="ycdY"
                     /locus_tag="KW011_003493"
     CDS             complement(2863..3417)
                     /gene="ycdY"
                     /locus_tag="KW011_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HBH7661749.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3441..4178)
                     /gene="ycdX"
                     /locus_tag="KW011_003494"
     CDS             complement(3441..4178)
                     /gene="ycdX"
                     /locus_tag="KW011_003494"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HBH7661750.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4233..5171)
                     /gene="ghrA"
                     /locus_tag="KW011_003495"
     CDS             complement(4233..5171)
                     /gene="ghrA"
                     /locus_tag="KW011_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HBH7661751.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5405..5492
                     /locus_tag="KW011_003496"
     tRNA            5405..5492
                     /locus_tag="KW011_003496"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5439..5441,aa:Ser,seq:gga)
     gene            5537..5673
                     /locus_tag="KW011_003497"
     ncRNA           5537..5673
                     /ncRNA_class="other"
                     /locus_tag="KW011_003497"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5764..6450)
                     /locus_tag="KW011_003498"
     CDS             complement(5764..6450)
                     /locus_tag="KW011_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HBH7661752.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(7031..7819)
                     /gene="phoH"
                     /locus_tag="KW011_003499"
     CDS             complement(7031..7819)
                     /gene="phoH"
                     /locus_tag="KW011_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HBH7661753.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(8440..9711)
                     /gene="efeB"
                     /locus_tag="KW011_003500"
     CDS             complement(8440..9711)
                     /gene="efeB"
                     /locus_tag="KW011_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HBH7661754.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(9717..10844)
                     /gene="efeO"
                     /locus_tag="KW011_003501"
     CDS             complement(9717..10844)
                     /gene="efeO"
                     /locus_tag="KW011_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HBH7661755.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10902..11732)
                     /locus_tag="KW011_003502"
     CDS             complement(10902..11732)
                     /locus_tag="KW011_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HBH7661756.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         3224 a         3167 c         2827 g         3003 t
ORIGIN      
        1 gacgatatat cttcaggctc tattattctt ctggatatga tggaagcgga taaaaagctt
       61 atccattatt ggcaggatac tttgagcagg aaaaacaaca atatcaaaat attgttgcta
      121 aatacgcctg aagattaccc gtaccgcgac attgaaaact ggcctcatat caacggcgtt
      181 ttttatgcca tggaggatca agaacgtgtt gtcaatgggt tgcaaggcgt cctgcgcggc
      241 gaatgctact ttacgcaaaa gcttgccagc tacttgatta cgcattcagg taactatcgt
      301 tataacagca cggaatcagc cctccttact catcgggaaa aagagatcct gaataaactg
      361 cgtatcggcg cgtctaataa cgagatcgct cgttcgttgt tcatcagcga aaatacggtt
      421 aaaacgcatc tttataatct tttcaagaag atagccgtaa aaaaccggac acaagcggtt
      481 tcctgggcaa acgataacct caggcgataa agccatgaaa cgttatttac gctggattgt
      541 ggcggcagaa tttctgttcg ccgcagggaa tcttcacgcc gttgaggtag aagtcccggg
      601 attgctaact gaccatactg tttcatctat tggccatgat ttttaccgag cctttagtga
      661 taaatgggaa agtgactata cgggtaactt aacgattaat gaaaggccca gtgcacgatg
      721 gggaagctgg atcactataa cggtcaatca ggacgttatt ttccagactt ttttatttcc
      781 gttgaaaaga gacttcgaga aaactgtcgt ctttgcactg attcaaactg aagaagcact
      841 aaatcgtcgc cagataaatc aggcgttatt aagtacgggc gatttggcgc atgatgaatt
      901 ctaaataaaa aattgttcgg aggctgcaat gcgtgtcaaa catgcagtag ttctactcat
      961 gcttatttcg ccattaagtt gggctggaac catgactttc cagttccgta atccaaactt
     1021 tggtggtaac ccaaataatg gcgctttttt attaaatagc gctcaggccc aaaactctta
     1081 taaagatccg agctataacg atgactttgg tattgaaaca ccctcagcgt tagataactt
     1141 tactcaggcc atccagtcac aaattttagg tgggctactg tcgaatatta ataccggtaa
     1201 accgggccgc atggtgacca acgattatat tgttgatatt gctaaccgcg atggtcaatt
     1261 gcagttgaac gtgacagatc gtaaaaccgg acaaacctcg accatccagg tttcgggttt
     1321 acaaaataac tcaaccgatt tttaagcccc agcttcataa ggaaaataat catgcagcgc
     1381 ttatttcttt tggttgccgt catgttactg agcggatgct taaccgcccc gcctaaagaa
     1441 gccgccagac cgacattaat gcctcgagct cagagctaca aagatttgac ccatctgcca
     1501 gcgccgacgg gtaagatctt tgtttcggta tacaacattc aggacgaaac cgggcaattt
     1561 aaaccctacc cggcaagtaa cttctccact gctgttccgc aaagcgccac ggcaatgctg
     1621 gtcacggcat tgaaagattc tcgctggttt ataccgctgg agcgtcagag tttacaaaac
     1681 ctgcttaacg agcgcaagat tattcgtgcg gcacaagaaa acggcacagt tgccattaat
     1741 aaccgaatcc cgctgcaatc tttaacggcg gcaaatatca tggttgaagg ttcgattatc
     1801 ggttatgaaa gcaacgttaa atcgggcggt gttggggcaa gatattttgg gatcggtgca
     1861 gacacgcaat accagctcga tcagattgcc gtgaacctgc gtgtcgtcaa tgtgagtacc
     1921 ggtgagatcc tctcctcggt gaacaccagt aagacgatac tttcctatga agttcaggcc
     1981 ggggttttcc gctttattga ctaccagcgc ttgctggaag gggaagtggg ttacacctca
     2041 aacgaacccg ttatgctgtg tctgatgtcg gctatcgaaa caggggtcat tttcctgatt
     2101 aatgatggta tcgaccgtgg tctgtgggat ttgcagaaca aagcagaacg gcagaatgac
     2161 attctggtta aataccgcca tatgtcggtt ccaccagaat cctgacagat aaaaatgccg
     2221 gaattaataa tccggcattt tactgtttaa gcgaaagcgt gagtgcccct cacgctttat
     2281 ttttgatttc ctgctcacgg tgagccgtct ttgcactacg cgccgccagc cacaggccgc
     2341 tgtttttcat tgcgtaacca aataccagcc caagcgccag cgaaggtaac accagtttcc
     2401 agtcaccctg cccggcaaac gtcgcgcatg cgcctataaa cgtccccgga acaaatgaaa
     2461 gcagcagctg tttggcctga atacacatca gaaacgccac aataccggtt atgacataac
     2521 cgagaatttc cagatgtggt gccagtgcac taccgtaaat aatgaccatc gcccacacca
     2581 cgccactaag cagggttgca gcggagatcg ccagcccttt cagcccacct tgcgggcagg
     2641 caaagtaggc cgtacagccc aagaagcccg cccagcttag taagcccagg gaaacagcca
     2701 cccatcccca gataccggag agaatgcctg ttgtgattgc aatagagaga agtatgttca
     2761 tgccgcgcat gatagcagaa aacgcggcca tgaatgagat cgagcacaca ttttatgcaa
     2821 caacaaatta tctgttgcag ttatgagtga cgatgatcac atttactctt cagaatcttc
     2881 ttccagctcg tcccacattg cactaatggc atcacgggtt agcggtgcca tggtgcgcca
     2941 gaaaggcgtg gttgcatggg cctccacttt gccgaggaac gcaccacacc agggtaacag
     3001 atactcactg aacagtgttt ccagtgcttc gctctcatct tccgttgact gatcttccag
     3061 ccaggaagcc gcaagcagca atgtgccgat gtgatcggct ggcgtatccg ccagtggcat
     3121 ccctcgttcg gaaagaaaag cgcgcacttc cgcttccgtt acgtcctcaa cccatgcgct
     3181 acgatatggc ggcactgcac attcatcgcc gataaacaac gcattgtaat cggcagagac
     3241 ttgcgccata tcacagcttt tctgtaaacg cgtcagtaac tcatcctgct ccagtggcca
     3301 gttcgcagcc agtttcccct cacgaatcag ggtaaacagc ggcaccagta aaggatcttg
     3361 cggttggcgg taatagagcg aacccagcac acgacagagg atagaaaact cgttcattaa
     3421 tatatttcca ttactagtaa ttaaaggtct gcaaattccg caatcggtgc cataccgcga
     3481 gattcaagga agttcagtaa gcggcgcgga gaaacattca aaatgcgctc tggcggaaaa
     3541 tctaccgcgt cgaggatttt aagacactct tcaaattccc ccatggtaaa cgcggtgtga
     3601 gaatccgagc ctaatgccac ccaacctcca gcatcacgta ccgctgcggc taccgcacga
     3661 cagttgtctt cactgccctt acgtgagtgt aaaaatgaag aattattgat ttccagcgcc
     3721 acctgatgtt tcgcggctgc ttcagcaacg gctttcacat ctatttcata tttgggattt
     3781 ccgggatggc ttattatatg cacattgccg ctggcgatag tggcgatcat cgcttgtgta
     3841 ttggtcgctt tgtcatgtgg tgcaaaaacc ggctcatgaa aaccggcaat aatcagatcc
     3901 agcgagtcaa acattttacc gctgcaatca atttcaccat caacattttt aatgttagct
     3961 tcgatgccgc gcaggatccc taccccatca accactcgcg gccagatacg catgttaatg
     4021 aagtgccagt gatgcggagc atcttccata tccgggccat gatcggtgat cgcaaaaagt
     4081 ttaatgccct tttgtttggc ctgggcaatg taatcactca atgtgctata tgcatgtgtg
     4141 ctggcaacgg tatgcatatg aaggtcgacg ggatacataa ctctctcctg tattcaattc
     4201 ccgccaagga tagcaggaat cctgatgctt tattagtagc cgcgtgcgcg gtcgacttgc
     4261 ccgcagaccc tctccccttt ttcgagctgg gcaatggtgc gagaaatgta ctccacagct
     4321 tcagcgggac gggtaatcgc ggcgacatgt ggtgttatcg tcacgcgtgg atgttgccag
     4381 agcggacttt caggcggtaa gggttcacga ttaaaaacat ccaacattgc gcctttaact
     4441 ttgccgctat ccagcgccgc gagcaggtca tcttccacaa catgaacacc acgcgccagg
     4501 ttgaggagat acgcgccatc cggtaatttt tcgagtaatt gttgattaat aatgccgacg
     4561 gtttcagggg tattcggtaa caaattaatc aatacccgac attggctcag aaatgcagac
     4621 agttcttccc gtccggcaaa gctttgcacg ccaggccacg atttacgggt tcgactccag
     4681 caacgcagcg gaaagcgcca ggtttgcaga ctctgagcaa ctttactgcc cagtacgcct
     4741 gcgcccaaaa tgccgatggt aaaatcttcc cgatgatatt caggcagcgg ttgccaatgc
     4801 gaactatttt gctggatgcg ataatcgtca aaacgtcgaa accaatgcag cacctgactg
     4861 acagcatatt cctgcatttg ctcgcccata ccggtatctt ccaggcgaaa aagtggaaca
     4921 gaagggttca gcatttcagg gtgtgcctgt agcttgctca aaatagaatc aacaccggcc
     4981 ccgagtgcga acaccgcttt aagatcgcgc cctgccagca tttcaacagg aggatgccag
     5041 actaaagcat aatcagcaga gtcattatct ccgcttttcc atgctctgac tcttgcctga
     5101 ggaatagctt tgcgcagtgc ctcaatccac cattgggtat cgaacgttgg gtgataaaag
     5161 atgatatcca tactgactcc cgaaaagcgt tgtgcgaaat ttattcgcac ttatcgttat
     5221 gatctacaaa ggccaccagc ataacaaatc cgtggtcggt ggcaaaaaaa gcagatttcg
     5281 cttattaaaa ccacacattg attgaagttt gaataaacgc gcgatttttt caaaaagttt
     5341 gttgacctca ggtcatgatt tccctaaatt agcgcccgtt ccagcaagac aggaacgaca
     5401 atttggtgag gtgtccgagt ggctgaagga gcacgcctgg aaagtgtgta tacggcaacg
     5461 tatcgggggt tcgaatcccc ccctcaccgc catatttaaa gaagagctcg tacgaaagta
     5521 cgggcttttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccggggttcg
     5581 acaactggcg acagccagtt ggacagaccg tgaacgcagt gagcgggctg cccgcagggc
     5641 gagcgaagcg agtcaatccc cccctcaccg ccatctttaa agaagagctc gtacgaaggt
     5701 acgggctttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccgggatact
     5761 tagttaatgc accggttcac tctttgtcgc cgcgactaat gggcgaattt tgtcacgagt
     5821 ctcccagacc ggaggaattt cctccaattt agtgacagaa gctcgtacaa aaggcacccg
     5881 tgcaagagtc ataattgtat ccaaccaacg gtcacgaata atgcgatccg ccctttcatg
     5941 actcgggtca tccagctcaa taacacagac tattttattg ttcttctttg aaaccagaac
     6001 ataatccatt gccatgccat tataaattgc aaagaaacgc tttctttctt cttcgctaaa
     6061 ctcaggttgt tctggaaagt tgatcaagcg ccccagagag acctggcaat ggatatgaca
     6121 ataatgtcca aaccattttt ccagattatc gaaatacatt ttttcacaac gcgtgattaa
     6181 ccactctttt ctttccaata aaataccttc ggcaaccatt ccctgaaata actcatctcg
     6241 ctcttgccaa cgagagtcac gataggtttt ctgcggtata tagctccttg tcgacgcctt
     6301 agcggcaggg gaagttgcag atgaagggac gggttgcgat ttagcgatga ttaccgatga
     6361 ggcaactttt cggagtgtca attttctggc aacagcaaaa ccggcgataa atgcaattgt
     6421 caaacaagtt acaaaagcca taaagctcat aattaatttt cacctatcca tgttgattat
     6481 atttcattac caatgttact cgctgcagcg tgttacatgc caacaatgca acgatcaata
     6541 tatagttagt atcatgtttt gattattaca accatcccta tttagccatt gcggcttgcc
     6601 ttccacaaaa aaataacaac acacaatatt agcaaagaat taattaagat taaccttaca
     6661 tttataatat tatccctaaa gaataaatca gaggaatata ttcggtgtta aacagtcagt
     6721 caaagtatag cccattgttg attaaacctc ataagcaaaa ttggcaccag atctcctgta
     6781 caggttgaat ggccgaaaaa agataggcag atgctcattt ttttggcttc tggaagaaaa
     6841 actctccgcg ctggcagtat tacactggtc tgatgctatg acgaaaaatg cctggtaaga
     6901 gtatctaaac cagactccag ttcagcttat caacacgctc aaagtcgccc ccctgaacgc
     6961 taacctgaag tgaaaatctg ttgaaataaa acaaagcccg gttcgcccgg gctctgcacc
     7021 gataacacac ttaactgtag gcatgcagcg tacgttggca aagtgccgaa cgtacgcagt
     7081 cctctttacc gaaccggacg atcccaacca tttcatcttc ttcgaaacgt tccagcgcgt
     7141 cacttaatcc ggagcacacg ccgcgaggca aatcgcattg cgtgatatca ccgttgacga
     7201 taaccgtcac gttctccccg aggcgggtta aaaacatttt catttgcgcg gcagtcacat
     7261 tctgcgcctc gtcaagaatg acgactgcat tttcaaaggt acgtccacgc atataggcga
     7321 acggcgcaat ttccaccttc cctatttccg gtcgcaggca gtactgcata aaggaagccc
     7381 ctaagcgccg gaccagcacg tcgtagaccg ggcgaaaata gggagcaaac ttttctgcga
     7441 tatctccagg taagaagcca agatcttcat cggcttgcag aactggacgg gtgacgataa
     7501 tcctgtcgac atccttatgt atcagggcct ctgccgcttt tgctgcgctg atccaggttt
     7561 ttccgccccc ggcttcgccc gtggcgaata tcagctgctt actctcaata gccttcagat
     7621 agtgcaattg cgcttcattt cgcgcgagga tgggcgaagt atcgcgactg tcgcgggcca
     7681 taccaatggc ttctacgccg cccatctgca caagcgaggt gaccgattct tcttcacgct
     7741 gcttatggct gcgcgaatcc cgtctcagca cacgttttgc ctcgcgacga gctttgatca
     7801 ctgctttttg tcttcccata gagagcacct tgagttgttt gtattcatca cacgcgccgt
     7861 tggcagcgcg attatgcgca cgaacatcag agggttggct tccttgtaag ccatagtttg
     7921 cttttggata aaatgccgaa aaacggctac gcgcaccgtt tacggcgtcg gtaacacatg
     7981 aaaagaaagg atgaggttga aaatgcaaag tgacgagatg actaccggag gagaaaactc
     8041 cgcgagtggt ggcgcgttga ttatctaaaa catgtccagt acaggacgtt accatccgcg
     8101 atctccatag tgactgacta tcactgccgg gaacttccgc tgctacttaa taagtacaac
     8161 agatctcgca tttattgcaa caatatattt acttatattt aactataaaa cactatttca
     8221 gtgacattag tttctactgg aaagatgaca gagtgatgac agtgatgaaa aaagctgtgt
     8281 gctttcagca ggatttgcat caggcatcag tgcacgatcg ccggatgcgg cgtgaacgcc
     8341 ttatccagcc taccaaaccc gcactgattg taggcatgat aagacgcgca agcgtcgcat
     8401 caggcatctg cgcacaaatg ccttatccgc ctaaaaacat taaacccgca ataacgcgct
     8461 tccgagataa tcgttcgcgt ccttcactcc cggcagcgaa aaaaaataac cgccgccgat
     8521 aggtttaacg tattcctcca gcgcttcgcc attgagcctt ttttgtactg tcaggaagcc
     8581 tttttccaga tcgtgttggt agcagacaaa cagcaacccc atatccagtt gtccggagtt
     8641 ggtgaccccc aacgaataac tgtagccgcg acgcagcatc aggctggact cactctccgc
     8701 cgtgcgggga ttcgccagcc ggatatggct gtccagcgca atcaccttcc cttccgggtc
     8761 gctggcgtaa tcaggcacat catgctcatg ctgcattccc agcggcgcac cggtttgctt
     8821 atcacggcca aaaatcgtct gctgttcttt cagcggcgtt ctgtcccaaa attccactcg
     8881 aaactgaatc aagcgtactg cctgatagct gccaccgatt gtccacgcag gctcctgctg
     8941 atctgccgtt acccacacca ctttttgcat caacttatca ttctggctat cgggattggc
     9001 agtgccgtct ttgaaaccca gcaaattaat cggcgtctct ttgcctttac tacgcgacac
     9061 gtgatcggaa ataaaccctt cccgcttcca gcgcacactg agcaaatccg gcgtgtgttt
     9121 gatgatatcg cgcagcgcat ggataaccgt gtcctgggtg ttggcgcaaa tctgtagcaa
     9181 cacatcacca tgacataacg ccgcatccag cgagtcgttg gggaaacgcg tcatcttctg
     9241 cagctttttt ggcatctgtg gcgcaaggcc aaagcgctca tcaaacaatg agtgacccac
     9301 cgataacgtg atggtgagat tatcgggcgc aatgtagccg ccaagaatgc cggaatcgag
     9361 tggtggcagg cgcggatttg gcgtttctgg tgctgctccg ccctgagtca gaaaagcaaa
     9421 acgctgagtc aacaagcgaa acaaccgctc aagatcggct ttatcgctgg caagcacatc
     9481 aaacgccacc agcatcattg cggcctgttg tggcgtcagg atccctgcct gatgctcacc
     9541 ataaaacggc tgtttctcat tgcgtgcatc cggtgaaagc gtgcccggtg cactttgcgt
     9601 tttttgtgca tgagcgaccg gacaacttcc cgccagcgcc agcgcaccaa tccctttcag
     9661 taaacgtcgg cgtgacggtt cattcacgcc gttttcatct ttatactgca taacgcttaa
     9721 tccagtccca gcacaccgcg aagttgcgcc agatcttccg ccagcgcagt aatcggtcct
     9781 ttcagtgcat tccggtcggc atcggtcaat ttgtcgtagg tttcaaaacc gtctttagta
     9841 cggtatttcg ccagaatggt atcgaccttt ttaaagttgg catcgacttt tgccagcagt
     9901 tccgggttgg ctttttgcag ttgtggacgc agcaaatcga caattttctg cgagccttca
     9961 acgttagcct ggaaatccca cagatcggtg tggctgtagc gatcttcttc accgctaatt
    10021 ttgctggctg ccacttcctc aatcagtccg gctgcgccgc cgaccacttt tgaaggtggg
    10081 aaagccagtt cactgatgcg tttttgcaaa tcgaccacat ctgtataaag ctgctcagcg
    10141 tactgatcca tccctttggt ggtgttgtcg ccaaacaatg ctttttccag acggtggaaa
    10201 ccagtgaatt ttgggtcggc ggctttttgc tcgtaatcat cttcacgggc gtcaatgctg
    10261 ccatccagat cggagaacag ttcagcaatc ggttcaatgc gctcatagtg ctggcgcgtc
    10321 ggcgcataca gtgctttcgc tttttcgata tcgcctgctt taatcgcgtc ggtaaaggct
    10381 ttggtgtcgg tcaccagctg cgtggtttcc gccatgacat acgctttata tgcagtaatt
    10441 gcaccaccaa gacttaacag cgcatcactt tgcgccgcat ccgccgttgc ctcacctttg
    10501 acgatcaact tccctttcgg gttagtcagc agaccgcagg tcatatcgta ttcgccaggc
    10561 tgtaaattcg ccgtcatttt ctggctaaaa ccaggggcga tattttcccg ctcttccacc
    10621 accatcacgc ctttgaggat ctcccactcc agcgccttct ggctatggtt ctgaataatg
    10681 aactgtgttt tcccggcgtt aaccgtaatg gtcatcggtt cgcactgctt atccgtcacg
    10741 gtcactttga cctgcggcac atcagcggcg ttagccataa aagcagaaga aaacagcgca
    10801 gccacgctca actgcaatgc gttacggcgg aagttaatgg tcatgacgag actatccctt
    10861 taaagtatgt tgtaactaag caagagtttg cgtcgtattt gttacgcgga gcgagacgct
    10921 gtcgcccctg cgcgtggtgg cagagcaaat gccaccagcg ccgggatgag ataaataaac
    10981 cagacggcga cttcgctgac gctcggtgct tcctgatagc caaaaatccc ttccatcagc
    11041 gtgccaaaca gcgagtgagt tgagagcact gcgctcatat cgaaggcgat ttcctgaaag
    11101 tggttccaca atccggcttc atgaaatgcg cgaatggcac cagcagccag ccctgcggcg
    11161 acgaagagaa taaacaggct ggtccattta aaaaatgcac caagattgag gcgaataccg
    11221 ccccagtaga gcaggaagcc tagcaccacg gcagtagcaa gaccgagcat tgcacccagc
    11281 ggcggccaga tcccgacatc ttgttgaaat gccgccagca ggaaaaagac cgactccagc
    11341 ccttcccttg caacggcaaa aaagaccatc atcaccagcg cccagccatg atgatttcca
    11401 cgctgcaatg cgctatcgac tgcctgctcc agttgtactt tgacgttgcg cgacactttg
    11461 cgcatccaga aaaccatcca ggtaaggatc accacggcga tcaccgccac gataccttca
    11521 aacagttcct gttctttttg cggaaattcg ccggtggttt cgttaatgaa gatacccaag
    11581 cccaggcaca acgcagcggc aagcaacacg ccaatccaca tcacgccaat ccatcggcct
    11641 cgctgggtac gcttaagata gctggcaatc aaactgacaa tcagcgcggc ttcaagtcct
    11701 tcgcgcaaca taatgagaaa cggaacaaac atgccaccca cccttaaacg tttttctgag
    11761 tcaatccatg caaagaaaag taaatcgcag tgatagcgat tatcattacg gcaaagaaaa
    11821 atacaagcgg aatgtttata aaatgatgtt ggaatgtaaa caaagagagg taataccagg
    11881 ggatataaac gattattcca ggtacaaaga tgggattgtt tacaacgagc catgtgttct
    11941 tgccagatgc cgcgtaaacg ccttatccag ccgacaaaac tgtccagact caatgcatca
    12001 cagcttccat gtaaaactga taagcgggtg tatcaagcaa tttcgtccat ccgcgattat
    12061 caaatctaac tttttgcccg gaacataata acagcgcggg tgttggtggc gtcaaaatcg
    12121 ctgaaacgtt ccccgaaggc cggggcagcc cacatggatg tgggctgagg gcgcgtctta
    12181 caggacgtta cctcgcgccc gacccggtag ccgaagggga t
//
LOCUS       DAESHI010000123        12079 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.124, whole genome shotgun sequence.
ACCESSION   DAESHI010000123 DAESHI010000000
VERSION     DAESHI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12079
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.124"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..952)
                     /gene="ydcO"
                     /locus_tag="KW011_003503"
     CDS             complement(<1..952)
                     /gene="ydcO"
                     /locus_tag="KW011_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HBH7661757.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLF"
     gene            1005..1541
                     /gene="sutR"
                     /locus_tag="KW011_003504"
     CDS             1005..1541
                     /gene="sutR"
                     /locus_tag="KW011_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HBH7661758.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1614..3575
                     /gene="rlhA"
                     /locus_tag="KW011_003505"
     CDS             1614..3575
                     /gene="rlhA"
                     /locus_tag="KW011_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HBH7661759.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3667..3897)
                     /locus_tag="KW011_003506"
     CDS             complement(3667..3897)
                     /locus_tag="KW011_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HBH7661760.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4119..4273
                     /locus_tag="KW011_003507"
                     /pseudo
     CDS             4119..4273
                     /locus_tag="KW011_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            4319..4735
                     /gene="hicB"
                     /locus_tag="KW011_003508"
     CDS             4319..4735
                     /gene="hicB"
                     /locus_tag="KW011_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HBH7661761.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            4814..6220
                     /locus_tag="KW011_003509"
                     /pseudo
     CDS             4814..6220
                     /locus_tag="KW011_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            6465..7610
                     /locus_tag="KW011_003510"
     CDS             6465..7610
                     /locus_tag="KW011_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH7661762.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            7628..8641
                     /locus_tag="KW011_003511"
     CDS             7628..8641
                     /locus_tag="KW011_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH7661763.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKI
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            8642..9542
                     /locus_tag="KW011_003512"
                     /pseudo
     CDS             8642..9542
                     /locus_tag="KW011_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            9532..10326
                     /locus_tag="KW011_003513"
     CDS             9532..10326
                     /locus_tag="KW011_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH7661764.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     LLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10348..11772
                     /gene="patD"
                     /locus_tag="KW011_003514"
     CDS             10348..11772
                     /gene="patD"
                     /locus_tag="KW011_003514"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HBH7661765.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            11982..>12079
                     /locus_tag="KW011_003515"
     CDS             11982..>12079
                     /locus_tag="KW011_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HBH7661766.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNK"
BASE COUNT         2947 a         2912 c         3223 g         2997 t
ORIGIN      
        1 caaacagacc tgcgagcaaa tagaaaatgc ctgcaacggc agctgccagc caacgttgat
       61 ctttatccgg atgcgcttcc gggctttggc aaatagccgc ggtgattgcc gcaataccga
      121 cggaataaac gccgaaaggg gaaaaaacca gtgccagcaa tccagtaaat acaattaatg
      181 gcgaaacagg agccgaatat ccagctgctt tcattgctgc gatacccggt gcgttttgcg
      241 atgccatcgt caccagaaaa agggggagtg caacgctcag gctgtgagca aacgaaaaat
      301 caggggtaat ataagtgggg agaacgggtt taaagacaac atcagttgtg acaacgtcac
      361 cttgcgcgat aacgatcacg atcccaataa tcatcgcggc aattaccgca tagcgcggcg
      421 caacggcctt ggttgccagc cataccagca acatacttcc acacaacgta aattgaccgt
      481 ccagactggc aaacgcctgt aaaccaaagc gtaataaaat cccggcaagc attgccgccg
      541 caagcgagtg cggaataatg cgcatcagac gagcaaagag tcccgttatg ccgcagagga
      601 ctattaacgc gttggtgaca ataaaaacgc cgatggcttc gttaagtgtt agtccctgca
      661 atccggtgac caacaaagcc gcgccaggcg ttgaccatgc ggtgagaaca ggtacgcgat
      721 accataatgt cagagtcagc gtactgacgc ccattgccag ccccagcgcc gtcatccagc
      781 cagagatttg tgcagtggtg gctccggcga caatcgctgc ttgccagatt attgccgctg
      841 aactggcgta gccaattaat accgccagaa accccgccag tagcgtgggt ggaggaatag
      901 aaaacagacg catagtcacc tcgtgcgtta tagcgtacag gcaccgtagc atttgtccgt
      961 tataacgcac aagtgataaa cttccgtttt gccggaggag tcgcatggaa aatctcgctc
     1021 gctttttatc cactacactt aaacaactac gccagcagcg cggctggagt ctttcgcgac
     1081 tggcagaagc gacgggcgtt tctaaagcaa tgttagggca gattgagcgt aatgagtcca
     1141 gtccgacggt cgcgacatta tggaaaattg ccaccgggtt gaacgtacca ttttcaacat
     1201 ttatttctcc gccgcagtcc gctatgcctt cagtttatga tccacaacag caggcgatgg
     1261 tgatcacttc gctgtttcct tatgatccac aactctgctt tgaacacttc tcgattcaga
     1321 tggcatctgg tgcaataagc gaatcgacgc cccatgagaa gggggtaatc gaacatgtcg
     1381 tcgtcattga tggacaactt gatctgtgcg ttgatgggga atggcagacc cttaattgtg
     1441 gggaaggcgt tcgatttgct gcagacgtca cgcatatcta tcgtaatggt ggggagcaaa
     1501 ccgtacattt tcattccctc atccattacc cgcgcagtta agcgggaaaa ctatttcgca
     1561 acgtcgcgct tgtggctaaa atagccgcca tttttcagct actggataag aatgtgaccg
     1621 tatcttctca tcgacttgaa ctgttaagcc cggcacgcga tgccgccatt gcccgcgaag
     1681 ctattttgca cggtgccgat gctgtttata tcggcggccc tggttttggt gcccgtcata
     1741 atgccagtaa tagcctgaaa gatattgccg agctggtgcc gtttgcccat cgttatggtg
     1801 caaaaatttt cgtcacgctt aacaccattt tgcatgatga tgagctggaa cccgcgcaac
     1861 ggctgattac tgacctctac cagaccggtg tcgatgcgct gattgttcag gatatgggga
     1921 ttctggaact tgatattccg ccgattgaac tgcacgccag tacgcagtgc gacattcgta
     1981 cagttgaaaa agcgaagttc ctctctgatg ttggcttcac gcagattgtg ctggcgcgag
     2041 agctgaatct tgatcagatc cgcgcgattc accaggctac ggacgcgacc attgaattct
     2101 ttattcatgg ggcactgtgc gtggcctatt cgggtcagtg ctacatttct catgcgcaaa
     2161 cagggcgtag cgccaaccgt ggcgattgct cgcaggcgtg ccgtttgcca tacacattga
     2221 aagacgatca ggggcgggtg gtttcctatg aaaaacatct gctgtcgatg aaagataacg
     2281 atcagactgc taacctcggc gcgctgattg atgctggcgt acgctccttc aagattgaag
     2341 ggcgttacaa agatatgagc tacgtgaaga atatcaccgc ccattatcgc cagatgctgg
     2401 atgccattat tgaagaacgt ggcgatctga cgcgtgcttc atcaggtcgt actgaacatt
     2461 tctttgttcc atcgacggaa aagactttcc accgtggtag cacagattat tttgtgaatg
     2521 cccgtaaagg cgatattggc gcgttcgatt cgccgaaatt tatcggcctg ccggtgggtg
     2581 aagtattgaa agtggcgaaa gatcatctca atgttgccgt caccgagcca ctggcaaatg
     2641 gtgatggcct gaacgtgatg attaaacgtg aagtcatcgg ttttcgtgcc aatacggtcg
     2701 agaaaaccgg agaaaatcag taccgcgtct ggcccaatga aatgccagca gatttgcaca
     2761 aaattcgtcc acatcaccca ctaaaccgta atcttgatca taactggcag caggcactga
     2821 caaaaacctc tagcgaacgt cgggtggcgg tagacattga actgggcggc tggcaggaac
     2881 aactgattct gaccctcacc agtgaagagg gtgtcagcat cacgcatacg ctggacgggc
     2941 agttcgacga agccaataac gccgaaaaag caatgaacaa tctgaaggat ggtctggcaa
     3001 aactggggca aaccctctat tacgcccgcg atgtgcaaat taatttgccg ggggcgctgt
     3061 ttgtaccaaa cagtctgtta aaccagttcc gccgtgaagc tgctgacatg ctggatgctg
     3121 cgcgtcttgc cagttaccag cgcggcagcc gtaaaccggt tgctgatcct gcgccggttt
     3181 ttccgcaaac gcatctgagt ttcctcgcga acgtatacaa ccagaaagcg cgtgaatttt
     3241 atcatcgcta tggtgtgcag ctgattgacg cggcgtatga ggcacatgaa gagaagggcg
     3301 aagtcccggt gatgatcacc aagcattgtc tgcgctttgc ctttaatctg tgcccgaaac
     3361 aggcgaaagg caatatcaaa agctggaagg cgacgccaat gcaactggtt aacggcgatg
     3421 aagtattaac gctaaagttt gattgccgcc catgcgagat gcacgtcatt ggcaaaatca
     3481 aaaatcacat actgaaaatg ccgttaccgg gaagcgtagt ggcatccgta agtccggatg
     3541 agctgctgaa aacattgcct aagcgaaaag ggtaaaacgc cagttttctg gttactcaca
     3601 actcattgaa tctgcatgat attgcctgcc gggtaaggcg tcacgccgca tccggcatca
     3661 aacgactcaa cgttgtcccg cttctggttt gcgcgatttt tgccagtaaa aatgctcgcg
     3721 caaaccttcc gccgactctt ccgccacagc acgcaattca tcgctgtccg cttcatgacg
     3781 cagctgatga tccacattct ttacccacac aaattcatgt cctttgtgcc ctgccatgag
     3841 ttgtcctgag aacagcgcac acgttaataa gacaaccgat aacgccttcg taaacatcct
     3901 gccacctttt tcttaccttt tgccgctatg atgccgatcg tttcttgagg ttattattca
     3961 gttttgcaaa ttagcgcaaa gaaattctgg aatcttcctt cctgattttg cattgcattc
     4021 tgccgttgcg gcgatttagt gctattttcg agcaaattac acataggggt aaacacaaat
     4081 gtttacttta ttgatatact cagcggcagg gaggcgatgt gaaacaaagc gagttcagac
     4141 gttggctcga atctcagggc gtcgatgtag cgaatggccg caaccatttg aaactcaggt
     4201 ttcatgggag gcgcagtgtc atgccgcgtc actcctgcgt aaagcaatcc tgaaacaact
     4261 cggtttgagt taatcgccaa ttaaaaaggt taatgacatg cgagagacag tcgaaattat
     4321 gcgttatccc gtcactctta cacccgcgcc ggaaggcggt tatatggttt cttttgtgga
     4381 tatccctgaa gcgttgacgc agggcgaaac tgtcgctgaa gcgatggaag cggcaaaaga
     4441 tgctttactg accgcatttg atttttattt tgaagataac gagcttatcc cgttaccttc
     4501 gccattaaat agtcatgatc actttattga agtacctttg agcgtcgcct ctaaagtatt
     4561 gctgttaaat gcttttttac agtcagaaat cactcagcaa gagttagcca ggcgaattgg
     4621 caaacctaaa caggagatta ctcgcctatt taacttgcac catgcgacaa aaatcgacgc
     4681 cgtccagctt gcggcaaagg cgcttggcaa agagttatcg ctggtgatgg tttaattaca
     4741 gttaacgaaa agttgtcatt tttaacaact gatatagact gccgaatcat ctgcacataa
     4801 ttacgattcg ataatgaaaa aataccagca gcttgcagaa caattacgcg agcagattgc
     4861 gtcgggtatc tggcaacccg gcgatcgttt gccttcgttg cgtgaccagg tggcgctttc
     4921 aggcatgagc tttatgactg tcagccatgc ctatcagttg ctcgaaagtc agggatatat
     4981 tatcgcacga ccgcagtcgg gttattacgt tgcgccacag gcaataaaaa tgccgaaagc
     5041 gccagtcatt ccagtcactt gagatgaagc agtcgatatc aacacttata tttttgatat
     5101 gttgcaggcc agtcgcgaac cgtcggtcgt tccgtttgcc tcggcctttc ccgacccgcg
     5161 acttttcccc ctccaacaac taaaccgctc gctggcgcag gtaagcaaaa ccgccacagc
     5221 gatgagcgtg attgaaaact taccgccagg aaacgcagaa ctgcgtcagg ctattgctcg
     5281 tcgctatgcc ttacagggca tcaccatttc tcctgatgaa attgtcatta ctgccggggc
     5341 gttagaggca ttaaacctca gtttgcaagc ggtaactgaa ccgggcgatt gggtgatagt
     5401 agagaatcct tgtttctacg gtgcgttgca ggcgctggag cggctacggc tgaaggcgtt
     5461 atcggtggcg acggatgtta aagaagggat cgatcttcag gcgctggaac tggcgttgca
     5521 ggattatccg gtgaaagcgt gctggctgat gactaatagc cagaacccac tcggatttac
     5581 cttaacgccg caaaaaaaag cacaactggt ggcgttactc aatcagtaca acgtaacgct
     5641 gattgaagat gacgtttaca gcgaacttta ttttggacgg gaaaaaccgc tgcctgcgaa
     5701 agcgtgggat cgccacgatg gcgttttgca ttgctcttcg ttttcgaaat gtctggtgcc
     5761 tggttttcgt attggttggg tcgccgccgg aaaacatgca cgtaaaattc aacgcttgca
     5821 gttgatgagt acgctttcca ccagctcacc gatgcaactt gcgctggtgg attacctttc
     5881 cacgcgccga tacgacgccc atcttcgtcg cctgcgtcgc cagcttgcgg aacgtaaaca
     5941 acgtgcctgg caggcactgc tgcgttatct gcctgcggaa gttaaaattc atcatagtga
     6001 cagcggttat tttctctggc tggagctccc cgagccgtta gatgccggcg aattaagcct
     6061 ggcggcactg acgcatcata tcagtattgc gccgggtaaa atgttttcta ccggtgaaaa
     6121 ctggtcacgt ttttttcgtt ttaataccgc gtggcagtgg ggagaacgtg aagaacaggc
     6181 ggtaaaacaa ttaggcaaac ttattcaaga acggctgtaa tagcgtttaa tttaattcct
     6241 cttagattgg gtaatatgaa tttcgaatag cagtcatatt tcctaacccc ttgtctatac
     6301 tccagaagat aaccttacag acggcataat gcgcggtagc tcacaacctg attaaatttt
     6361 ctcagggacg aaggtgtgcc tgcaagccgc cgtctatggt taaacaagga gatattttta
     6421 cggcacggcg gctgaacaat taattacgac aggagtaaga ccttatgagc aagacatttg
     6481 cccgcagcag cctgtgtgcg ctcaccatga caataatgac cgctcacgcc gccgaaccac
     6541 ctaccaattt agataaaccg gaagggcgac tggatattat cgcctggccg ggatacatcg
     6601 aacgcggaca aactgataaa caatacgact gggtaacgca atttgaaaaa gagacaggct
     6661 gcgcggtgaa tgtgaaaacc gccgcgactt ccgatgaaat ggtcagtctg atgaccaaag
     6721 ggggttacga tctggttacg gcatccggcg atgcctcgct gcgtttgatt atgggcaaac
     6781 gcgtgcagcc gattaatacc gccttgattc ccaactggaa aacgctcgat ccgcgcgtgg
     6841 ttaaaggcga ctggtttaat gttggcggca aagtttacgg cacaccttac caatgggggc
     6901 cgaacctgct gatgtacaac actaaaacct tcccgacgcc gccggatagc tggcaagtgg
     6961 tttttgttga gcaaaatctg ccggacggca agagcaataa aggccgcgtt caggcttatg
     7021 atggccctat ctacattgcg gacgctgcgt tgttcgttaa agccactcag ccgcagttgg
     7081 gcatcagcga tccgtatcaa ctaaccgaag aacagtacca ggcggtgctg aaagtgctgc
     7141 gcgctcaaca cagtttgatc catcgctact ggcatgacac taccgtgcaa atgagcgatt
     7201 tcaaaaacga gggtgtggtt gcttccagtg cctggcccta tcaggccaac gccctgaaag
     7261 ccgaaggcca gcctgttgct accgttttcc cgaaggaggg tgttaccggt tgggctgata
     7321 ccaccatgct gcatagcgaa gcgaaacatc cggtttgcgc ctacaaatgg atgaactggt
     7381 cattaacgcc aaaagtgcag ggcgatgtgg cggcctggtt tggctcgtta ccggtagtgc
     7441 cggaagggtg taaagccagt ccgttattag gcgaaaaagg ttgtgaaacc aacggtttta
     7501 actatttcga caaaatcgcc ttctggaaaa cgcctatagc agaagggggc aagtttgttc
     7561 cctacagtcg ctggacgcag gattacattg ccattatggg cggtcgctaa cttcgctggg
     7621 gtgctttatg acgtacgcag tggagtttga caacgtctcg cggttgtacg gtgacgtgcg
     7681 cgcagtagat ggcgtcagta ttgcgataaa agatggtgag ttcttctcta tgctggggcc
     7741 gtccggctcc ggcaaaatca cctgcctgcg cctgattgct ggcttcgaac agctttccgg
     7801 cggggctatc tctatctttg gtaaacccgc cagcaatctg ccaccgtggg agcgggacgt
     7861 gaatactgtc tttcaggact acgcgctatt tccgcatatg tcgattcttg acaatgtcgc
     7921 ctatgggctg atggtcaaag gcgtgaataa aaagcagcgg cacgcaatgg cgcaagaggc
     7981 gctggagaaa gtggcgttgg ggtttgtaca gcaacgtaaa ccgtcacaac tttctggtgg
     8041 tcagcgccag cgggttgcta tcgccagagc attggtgaat gaaccgcgcg tattgctgtt
     8101 ggatgaaccg ctcggcgcac tggatctcaa attgcgtgag cagatgcagc tggaactgaa
     8161 aaaactgcaa cagtctctcg gtatcacttt tatcttcgtt acccacgatc agggcgaagc
     8221 gttatcgatg tccgatcgtg tggcggtttt caataatgga cgcattgagc aggtcgattc
     8281 cccgcgcgat ctctatatgc gcccgcgcac gccgtttgtt gccgggttcg ttggtacatc
     8341 gaatgttttt gatggactga tggcagagaa actttgtggc atgacgggaa gcttcgccct
     8401 gcgaccggaa catatccgcc tcaacacccc tggtgaactg caggccaatg gcacgatcca
     8461 ggcggtgcaa tatcagggcg cggcaactcg ttttgaactg aaattgaacg gcggtgaaaa
     8521 actgcttgtg agtcaggcca atatgacagg cgaagaactg cctgccacgc tcacgcccgg
     8581 acaacaggtg atggtttcct ggtcgcgtga tgtgatggtg ccgctggttg aggagaggtg
     8641 aatggcgatg aatgtattgc aatcaccttc acgtccaggt ctggggctgt ttttactgct
     8701 gcttggcccg ctaatgtggt ttggcattgt ctatttcggc tcgctgctga cactgttatg
     8761 gcagggattt tatacttttg acgatttcac catgtcggta acgccggaac tgacgctggc
     8821 gaatatccgt gcgctgttta atccggcgaa ttacgacatc attctccgca cgctgactat
     8881 ggctgtggca gtcactatcg ccagcgccat tctggctttt ccaatggcgt ggtatatggc
     8941 gcgctatacc agcgggaaaa tgaaagcgtt tttatattgc ggtaatgttg ccgatgtggg
     9001 cgagctacat tgttaaagcc tatgcctgga cgttattgct ggcaaaagat ggcgtggctc
     9061 agtggttttt acaacatctt gggctggaac cactgctgac tgcgttcctt acattacctg
     9121 cggtgggcgg aaatacgctg tcaacttccg ggctggggcg ctttctggtg tttctctata
     9181 tctggttgcc gttcatgatc ctgcccgttc aggcggcgct tgagcgtttg ccgccgtcat
     9241 tgttgcaggc gtcggctgat ctcggcgcac gtccacgaca aacctttcgc tatgtggtgc
     9301 tgccgctggc aatcccgggt attgccgctg gctctatctt taccttctca ctcacactgg
     9361 gcgattttat cgtcccgcag ctggttggtc ctccaggata ttttatcggc aatatggttt
     9421 attcccagca gggggcgatt ggcaatatgc cgatggcggc ggcattcacc ctggtgccga
     9481 ttattctcat cgcactgtac ctggcgttcg tgaaacgtct gggagcgttc gatgcactct
     9541 gaacgcgcac cgtttttcct caaactggcg gcctggggcg gcgttgtttt cctacatttt
     9601 cccatcctga taatcgccgc ctatgcgttt aacactgaag atgcggcgtt tagttttcca
     9661 ccgcagggcc tgacgctgcg ctggtttagc gtggcagcac agcgtagtga tattcttgat
     9721 gccgtgacac tgtcacttaa agtggcggcg ctggcgacat taattgcgct ggtgttaggg
     9781 acgctggcag ctgccgcgct gtggcgacga gactttttcg gcaaaaacgc catttcgctg
     9841 ttactgctgc tgcccattgc gctgccgggc agcgtcactg gtctggcgtt attaaccgcc
     9901 tttaaaacca tcaatctgga gccgggattt ttcaccatcg tggtcggtca tgcgactttt
     9961 tgtgtagttg tggtgtttaa caatgtcatc gcccgttttc gccgcacctc ctggagtctg
    10021 gttgaggcgt caatggatct tggggccaat ggctggcaaa ccttccgcta cgtagtgttg
    10081 ccgaatctca gttcggcgtt actggcagga ggaatgctgg cgtttgcctt gtcgttcgat
    10141 gaaatcatcg ttacgacctt tacggcaggt catgaacgaa cgttactgtt gtggttgctc
    10201 aatcagcttg ggcgaccgcg tgatgtaccg gtaactaacg tggtggcact gctggttatg
    10261 ttggtaacaa ccttgccgat cctgggggcc tggtggctaa cccgcgaagg cgacaatggt
    10321 caataaccac tgatacagga atatgctatg caacataagt tactgattaa cggagaactg
    10381 gttagcggcg aaggggaaaa acagcctgtc tataatccgg caacggggga cgttttactg
    10441 gaaattgccg aggcatccgc agagcaggtc gatgctgctg tgcgcgcggc agatgcagca
    10501 tttgccgaat gggggcaaac cacgccgaaa gtgcgtgcgg aatgtctgct gaaactggct
    10561 gatgttatcg aagaaaatgg tcaggttttt gccgaactgg agtcccgtaa ttgtggcaaa
    10621 ccgctgcata gtgcgttcaa tgatgaaatc ccggcgattg tcgatgtttt tcgctttttc
    10681 gcgggtgcgg cgcgctgtct gaatggtctg gcggcaggtg aatatcttga aggtcatact
    10741 tcgatgatcc gtcgcgatcc gttgggggtc gtggcttcta tcgcaccgtg gaattatccg
    10801 ctgatgatgg ccgcgtggaa acttgctccg gcgctggcgg cagggaactg cgtagtgctt
    10861 aaaccatcag aaattacccc gctgaccgcg ttgaagttgg cagagctggc gaaagatatc
    10921 ttcccggcag gcgtgattaa catactgttt ggcagaggca aaacggtggg tgatccgctg
    10981 accggtcatc ccaaagtgcg gatggtgtcg ctgacgggct ctatcgccac cggcgagcac
    11041 atcatcagcc ataccgcgtc gtccattaag cgtactcata tggaacttgg tggcaaagcg
    11101 ccagtgattg tttttgatga tgcggatatt gaagcagtgg tcgaaggtgt acgtacattt
    11161 ggctattaca atgctggaca ggattgtact gcggcttgtc ggatctacgc gcaaaaaggc
    11221 atttacgata cgctggtgga aaaactgggt gctgcggtgg caacgttaaa atctggtgcg
    11281 ccggatgacg agtctacgga gcttggacct ttaagctcgc tggcgcatct cgaacgcgtc
    11341 agcaaggcag tagaagaggc gaaagcgaca gggcacatca aagtgatcac tggcggtgaa
    11401 aagcgcaagg gtaatggcta ttactatgcg ccgacgctgc tggctggcgc attacaggac
    11461 gatgccatcg tgcaaaaaga ggtatttggt ccagtagtga gtgttacgcc cttcgacaac
    11521 gaagaacagg tggtgaactg ggcgaatgac agccagtacg gacttgcatc ttcggtatgg
    11581 acgaaagatg tgggcagggc gcatcgcgtc agcgcacggc tgcaatatgg ttgtacctgg
    11641 gtcaataccc atttcatgct ggtaagtgaa atgccgcacg gtgggcagaa actttctggt
    11701 tacggcaagg atatgtcact ttatgggctg gaggattaca ccgtcgtccg ccacgtcatg
    11761 gttaaacatt aagttagatg ttagtggctg ttataggacg gccactatcg ttttaggttc
    11821 tgagtttaat ggcggttaag tattagaatg attacattaa taactagcgt aaaaattagg
    11881 ctaaatgttt gatggcgatt tttcatcctg tcacagttga ttagcataag gctttagagt
    11941 atcgttatta aactaataat ttataagtag aagtattaac aatgaaagtt caattgtaca
    12001 aattcacgga agataaaaat aaaaccctta ccttcagatg gacgaaaaaa cactttgagt
    12061 tttgtatgga taataaaat
//
LOCUS       DAESHI010000124        11948 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.125, whole genome shotgun sequence.
ACCESSION   DAESHI010000124 DAESHI010000000
VERSION     DAESHI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11948
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.125"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="KW011_003516"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="KW011_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HBH7661767.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="KW011_003517"
     CDS             complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="KW011_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HBH7661768.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="KW011_003518"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="KW011_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HBH7661769.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="KW011_003519"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="KW011_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HBH7661770.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="KW011_003520"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="KW011_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HBH7661771.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="KW011_003521"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="KW011_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HBH7661772.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="KW011_003522"
     CDS             complement(6574..7338)
                     /locus_tag="KW011_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBH7661773.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="KW011_003523"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="KW011_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HBH7661774.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8641..10341
                     /gene="araB"
                     /locus_tag="KW011_003524"
     CDS             8641..10341
                     /gene="araB"
                     /locus_tag="KW011_003524"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HBH7661775.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10352..11854
                     /gene="araA"
                     /locus_tag="KW011_003525"
     CDS             10352..11854
                     /gene="araA"
                     /locus_tag="KW011_003525"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBH7661776.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
BASE COUNT         2797 a         3348 c         3167 g         2636 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatcaccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggcg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caggagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     8521 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     8581 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
     8641 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggaa
     8701 tgcgctaccg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg
     8761 caattttgtg atgccccgaa taaccagttc cgccatcatc cgcgtgacta cattgagtca
     8821 atggaagcgg cgctgaaaat cgtgcttgca gagcttagcg ccgaacagcg cgcagctgtg
     8881 gtcgggattg gcgttgacac aaccggctcg acgcccgcac cgattgacgc cgacggtaac
     8941 gtgctggcgc tgcgcccaga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
     9001 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
     9061 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
     9121 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
     9181 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
     9241 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
     9301 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
     9361 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
     9421 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
     9481 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
     9541 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
     9601 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
     9661 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
     9721 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
     9781 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
     9841 cgtcgcacgc caaacgctaa ccaacgtctg aaaggggtga ttaccgatct taacctcgct
     9901 accaacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
     9961 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    10021 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    10081 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    10141 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    10201 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    10261 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    10321 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    10381 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    10441 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    10501 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    10561 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    10621 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    10681 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    10741 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    10801 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa
    10861 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    10921 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    10981 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    11041 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    11101 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    11161 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    11221 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    11281 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    11341 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    11401 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    11461 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    11521 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    11581 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    11641 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    11701 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    11761 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    11821 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaacgcct
    11881 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    11941 aaagcgca
//
LOCUS       DAESHI010000125        11808 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.126, whole genome shotgun sequence.
ACCESSION   DAESHI010000125 DAESHI010000000
VERSION     DAESHI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11808
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.126"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..488
                     /locus_tag="KW011_003526"
     CDS             <1..488
                     /locus_tag="KW011_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH7661777.1"
                     /translation="NVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHH
                     RKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRR
                     NPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARL
                     S"
     gene            540..1475
                     /gene="ttcA"
                     /locus_tag="KW011_003527"
     CDS             540..1475
                     /gene="ttcA"
                     /locus_tag="KW011_003527"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBH7661778.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1604..2977)
                     /gene="dbpA"
                     /locus_tag="KW011_003528"
     CDS             complement(1604..2977)
                     /gene="dbpA"
                     /locus_tag="KW011_003528"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HBH7661779.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(3455..4438)
                     /gene="zntB"
                     /locus_tag="KW011_003529"
     CDS             complement(3455..4438)
                     /gene="zntB"
                     /locus_tag="KW011_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HBH7661780.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            4693..5925
                     /gene="dgcM"
                     /locus_tag="KW011_003530"
     CDS             4693..5925
                     /gene="dgcM"
                     /locus_tag="KW011_003530"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HBH7661781.1"
                     /translation="MIAHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5946..6509)
                     /gene="smrA"
                     /locus_tag="KW011_003531"
     CDS             complement(5946..6509)
                     /gene="smrA"
                     /locus_tag="KW011_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HBH7661782.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6839..7747)
                     /locus_tag="KW011_003532"
     CDS             complement(6839..7747)
                     /locus_tag="KW011_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH7661783.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            7923..9233
                     /gene="abgA"
                     /locus_tag="KW011_003533"
     CDS             7923..9233
                     /gene="abgA"
                     /locus_tag="KW011_003533"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HBH7661784.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            9233..10678
                     /gene="abgB"
                     /locus_tag="KW011_003534"
     CDS             9233..10678
                     /gene="abgB"
                     /locus_tag="KW011_003534"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HBH7661785.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            10715..>11808
                     /locus_tag="KW011_003535"
     CDS             10715..>11808
                     /locus_tag="KW011_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBH7661786.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSN"
BASE COUNT         3004 a         3001 c         2928 g         2875 t
ORIGIN      
        1 tgaatgtcag aagaaacctg acgatacttg atatgttcgg tcccccaaaa acaaatgccg
       61 ggatccggac ggtaacatta ctgcagccgg ctcttgaagc actgaaggag caatacaaac
      121 tgaccgggca tcatcgcaaa agcgaaatca ctttttatca tcgggagtac ggcagaactg
      181 aaaagcaaaa actgcatttt gttttcatgc ccagggtgtg taacgaaaaa cagaaacctt
      241 attactcggt aagcagtttg ggtgcaagat ggaatgcagc agtaaaacgt gctggtattc
      301 gccgccgtaa tccgtaccat acgcgacata cttttgcctg ctggctgttg acggcaggag
      361 cgaacccggc atttatagcc agccagatgg ggcatgaaac tgcgcaaatg gtgtatgaaa
      421 tttacggtat gtggattgat gacatgaacg acgaacagat agccatgttg aatgcgcggt
      481 tatcgtagtt gcaaagtttg cccccaattt gccccattta gtaccagaga actgaaataa
      541 tgcaagaaaa tcaacaaatt acaaagaaag aacaatacaa cctgaacaaa ttacaaaaac
      601 gtctgcgtcg taacgtgggc gaagccattg ctgacttcaa tatgattgaa gaaggcgatc
      661 gcatcatggt ttgcctctcc gggggtaaag acagctatac catgctggag attctgcgca
      721 atttgcagca aagcgcgcca atcaattttt cgctggtggc tgttaacctc gatcaaaagc
      781 aaccgggctt cccggaacac gttctgcccg agtatcttga aaagctgggc gttgagtaca
      841 agattgtcga agagaacact tacggtatcg tgaaagagaa gattccagag ggcaaaacca
      901 cttgctcact gtgttctcgc cttcgtcgcg gtatccttta tcgtaccgca acggaactgg
      961 gggcgacgaa gatcgcgctg ggtcaccatc gtgacgatat cctgcagacg ctgttcttaa
     1021 atatgttcta cggcggcaag atgaaaggta tgcctccgaa actgatgagc gatgatggca
     1081 aacatatcgt tattcgtccg ctggcctact gccgcgagaa agatattcag cgatttgccg
     1141 atgcaaaagc gttcccgatt attccgtgca acctgtgcgg ttcacagcct aacctgcaac
     1201 gtcaggtgat tgctgacatg ttgcgtgact gggataaacg ttatccaggg cgtatcgaga
     1261 cgatgttcag cgcgatgcag aatgtggtgc cgtcgcatct gtgcgatacc aacctgttcg
     1321 atttcaaagg catcacccac ggttctgaag tggttaacgg gggtgatctg gcgtttgatc
     1381 gcgaagagat cccactacaa ccggcgggct ggcagccaga agaagatgaa aatcagttgg
     1441 atgagttacg gctgaatgtg gttgaagtga aataaccagg atggcgcccg atgcgcaagc
     1501 gtatcgggct actcttatgg aggccggata agacgcggcc agcgtcgcat ccggcaatcc
     1561 cgaataagat gtttactctt gcacccggca attcaacatt tcattatttt aataaccgca
     1621 cccggctcgt ttttccttta atcttcccgc cctgtaactg tttccatgct ttatgagcaa
     1681 cagcctgacg gaccgcgaca tagacatgcg ccggatgcac ggcgattttg ccaatatctg
     1741 cgccatcaag cccgatatct cctgtcagtg cacctaatac atcacccggg cgcattttgg
     1801 cttttttccc accatcgata cacaacgttg ccatttctgc ttccagcggc acaatggaac
     1861 tattagctgg cggcgtttgc cagttaagtt ttatctgcaa catgtcagaa atgatattgg
     1921 cccgctgtgc ttcttccgga gcacagaaac tgatcgccag accgctattt cctgcacgag
     1981 ctgtacggcc gatgcgatgt acatgaactt cagggtccca cgccagctca aagttcacca
     2041 ccagctcaag cgatttaata tccagaccac gcgcagcaac atcagtcgca accagtacac
     2101 gggcgctacc gttagcaaaa cgtaccaggg tctgatcgcg atcgcgttgc tccaaatcgc
     2161 cgtgtaatga caatgcactt tgccctactt cattcagcgc gtcgcagaca gcctggcaat
     2221 cttttttggt attgcaaaac accacgcaag aggatggctg atgcaagctt aataaccgtt
     2281 gcaacagagg aattttgcct ttgctggatg tctcataaaa ttgttgttca atgggtggca
     2341 aagcatctgt tgagtcaatt tcaatcgcca aaggatcgcg ttgcactcgt ccgctgatta
     2401 cggcaatggc ttccggccag gttgccgaaa acagaagcgt ctgtcgagat gcaggcgcaa
     2461 aacggatgac atcatcaatg gcatcgctaa atcccatatc cagcatgcgg tcggcctcat
     2521 ccatcaccaa agtattcagc gcatccagtg ataccgtgcc tttttgcaag tgatccagca
     2581 aacgacccgg cgttgccacg ataatatgcg gcgcatgttg caacgaatca cgctgcatac
     2641 cgaacggttg accaccgcac aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc
     2701 gacgcaattc acctgccacc tgatccgcca gttcacgcgt aggacacagc actaaagcct
     2761 gggtttgaaa tagcgacgca tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt
     2821 tgccgctgcc ggttttcgcc tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg
     2881 cggcggcctg caccggcgtc atagttaaat aacccaactc attaaggttc gtgagttggg
     2941 cgggaggcaa aacattcagg gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac
     3001 gcaaagttag caggcgcgta tcctcgcaga tctacgctca cgatgcgaca atttaatcgg
     3061 ttcttcatcg ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggcactgg
     3121 aacaggatcg ccaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat
     3181 atttccctcc ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa
     3241 aaaaagccga ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa
     3301 aaaaggaagt aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca
     3361 agggagatat tgccctgaat gggtagagag tttattgact tcgctcaaac tttgcggcgt
     3421 ttttgtatac agacagccgg aaaaattgct tttgttacaa ccatttacta cggtgcaacc
     3481 ataaagcaac accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt
     3541 gccacccgcc gccagggatc ccaccaaggt tgacgccaaa taacccggtc agaaaggtac
     3601 tgggtaaaaa gaccattgcc atcaacgaca ttgtataggt acgacgagct aaattttcct
     3661 gcatcacctg agcgatttca tccgccatca cgccagtccg tgctatacag gcgtcgattt
     3721 cgtcaaggcc gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc
     3781 tcatccacgg caaacgttca ctggcaagac gagcataaac atcacgttgc ggtgccatat
     3841 agcgacgcat cacaattaat tgtttgcgca gcagagccag gaatccacgc ggtggaattt
     3901 gctgatcaag gagattatct tcaaggtcga taattttatc gtgcagctgc tcgataaatt
     3961 cgctggaatg atcggtcaac gcatcgcaca catccaccag ccaccctccg caatcggtcg
     4021 gacccgtgcc ctcttccaga tcgctcacca catcgtccag cgccagcact ttgcgttgtc
     4081 gggtcgaaac aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc
     4141 gttcatcggt gctgccgttt atacagcgca atgtaatcag cgtgccttca ccgagacggc
     4201 tgactcgggg ccgcgtgctc tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg
     4261 gtgttgtcgc cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg
     4321 gatgcgcttc atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca
     4381 gcatccaggc aaatactgca tccgggacat taacgtccga tcccttaatc gcttccacag
     4441 tgcctccatc atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc
     4501 tcatcacccc attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc
     4561 aaaatgcttt tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa
     4621 ttatctgatc atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat
     4681 aacgggatct caatgattgc gcacaacttc aataccctgg acttactcac cagtcctgtc
     4741 tggatcgttt cgccctttga ggaacagtta atttatgcca atagcgcggc gcgactgttg
     4801 atgcaagacc tcacgtttag tcagctacga accggtccct attccgtctc ctcacaaaaa
     4861 gaactgccga aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcact
     4921 gttcagcgta aagaagagga aacagcatta aactgtcggc ttgttttgcg aaagctgaca
     4981 gaagcagaac cggtgattat tttcgaaggt atcgaagcgt cggcaacgct gggtttaaaa
     5041 gccagtcgct cggcaaatta tcagcgcaaa aaacaaggtt tttatgcgcg cttttttctg
     5101 actaactctg caccaatgtt gttgattgac ccctcacgag atggccaaat cgtcgatgct
     5161 aacctcgccg cgctcaattt ctatggttat aaccatgaaa cgatgtgcca gaaacatacc
     5221 tgggaaataa atatgctcgg gcgtcgcgtc atgcctatca tgcatgaaat ttcgcattta
     5281 cccggtggtc ataaacctct gaattttatt cataagctgg cggatggttc gactcgtcat
     5341 gtgcagacct acgccgggcc gattgaaatt tatggcgaca agctcatgtt atgtattgta
     5401 catgatatta ctgagcaaaa acggctggag gagcagctgg aacatgctgc ccaccatgac
     5461 gcgatgaccg gattactgaa tcggcgacag ttttatcaca ttaccgagcc aggccaaatg
     5521 cagcatctcg ccatcgccca ggattacagc ttgttgctca tcgacaccga tcgttttaaa
     5581 cacattaacg atctctatgg gcattctaaa ggtgatgagg tgttatgcgc cctcgcccgc
     5641 accctcgaaa gttgcgctcg caaaggcgat ttggtgtttc gttggggagg cgaagagttt
     5701 gtcttattgc taccaagaac cccactggat accgcgcttt cgctggctga aactatccgc
     5761 gtaagcgtgg caaaagtgag tatttcgggc ttaccacgct ttaccgtcag cattggtgtg
     5821 gcgcatcacg aaggaaatga aagcatcgat gaactgttta aacgcgttga tgatgctttg
     5881 tatcgggcga aaaatgatgg acgcaaccgc gtgctggcgg cataagccgc ggatgcgtct
     5941 cgagatcaac gactgcgctt agcgtggcgc tcccagtttt cttgcttcgc ctgcgccgtt
     6001 ttacgtagtg cgacgtaaca cgccccgctg ccgccatgat gcggtagcgc agtgcaatat
     6061 gcctgaacat catcaaattc ggtcagccag cgcgccacat agctgcggac aatattggca
     6121 tgcgatttat catcccgtcc tttgccatga ataatcagca cgttacgcaa accatcagcc
     6181 atggcttgtt gaatgaaact gaacatcatt ttgcggcact cttccaccgg ctggcgcaaa
     6241 agattcaggc tcgcctgttg cggatattta ccactgcgca gcttatccag caccccatgt
     6301 tgcaaccctt cccgccgaaa ctccagcggc tgacttagtg ggatgatgtc gagaaatccg
     6361 gtggtgagga aattatcaag ctgcagcgtg tcgatacgct gcggcgcacg ttggttacgc
     6421 gttggatgcc agtggacatc ggtagcacgt ttcagcggct ggacatcttc catggcgtca
     6481 agaaacagcg atttgtcgtc aaggttcatg ttacatcctc ccgcaattaa gagcgcgata
     6541 tgataaccag accgggtcgg tccaacaacg tattacccaa aattccagta ataagttcca
     6601 aatattgccg atattttaag caaaatgctt atgcatgatt attcattcac gatattaata
     6661 atgtaactta tattttcgtg aaatctgtca ctgaagaaaa ttggcaacta aaggttaaaa
     6721 ccgttataac acagtcaccg gcgcagagga gacaatgccg gatttaagac gcggatgcac
     6781 tgctgtgtgt actgtagagt ctggcggatg tcgacagact ctattttttt atgcagtttt
     6841 aactttgcag atagccgcat tctcgccgga attgcgtgat taatgacgcg gtcagtggtg
     6901 tctggcgact atcacgccgc tgaatcaaat aataggccgc tttcggtaaa atttcgctaa
     6961 ccggcaacat caccagcccc tgtccgtgca agggatcgca gcccatttct tcaggcagta
     7021 tgctgagaaa atcgcttttt gccaccagac tgatacaggc tgagaacgtc tcgcagacta
     7081 caccgacctg tggcgtttgc gcctgatcgt caagcaattc actcaactgt ttgtagtaac
     7141 tgccgtgtgg cgtcggcatt gtccagctgt aatccagtaa ctgtttgatc gaacgggcac
     7201 caatggcggg gtgtcccggg cggcaaaaga tcgcgaattg cttttccagt aatttctcaa
     7261 aagtaaattc gtggtcgtac ggtccctgat aataggtatt gatggtgaaa tccaattctc
     7321 cctgacgcaa ttcattaatc atcgacacca gttgcccttc cataatgcgt acttttacct
     7381 gcggatgctg ctgatgaaaa cgagatatga cagctggcat cagactgcgg gaaatactgg
     7441 cccccatgcc gatattaatc tgccctgcca gttgcccttg tcgttggcga atatcctctt
     7501 gggctgcgcg cagctcttca agaataagac tggcgtgctg ataaaaactt tcaccggcat
     7561 cagttaacgt cacgccttta ctacggcgaa aaaagagttg cgccgctaat ccttcttcca
     7621 gctcctgaat agatttactc agtgccggtt gcgacatatt caacattcgg ctcgctccgc
     7681 gaatgctgcc ctgacgagcc acttcaacaa aagcccgaat ttgatgaatt tttacctgaa
     7741 aagccatgac gccaccgata accgttattt atcagaccaa agaaactggc atctacttta
     7801 atgcagatga ttgtgtcagg gtaatttatg aacggttaaa actgtgaaaa atcagttagt
     7861 gataagtaaa aactatcgct acgtgaaccg ggtcacactt tttactgatg acgggaaagg
     7921 ttatggagtc tttgaatcaa tttgttaatt cgcttgcccc aaaattatcg cactggcgac
     7981 gtgattttca tcactatgca gagtctggct gggtggaatt ccgcactgcc acccttgttg
     8041 cggaagaatt gcagcagctc ggctattcac tggcgctggg ccgcgaagta gttaatgaaa
     8101 gtagccggat gggactacct gatgaactca ctctacaacg cgaattcgag cgcgctcgtc
     8161 aacagggtgc gctagcacaa tggattgcgg tttttgaagg tggtttcact ggtatcgtcg
     8221 ccaccctgga taccggtcgc cccggtccgg tgatggcttt ccgtgtcgat atggacgcgc
     8281 tggatctcag tgaagagcag gatgtcagcc atcgccccta ccgcgacggt tttgcgtcat
     8341 gtaacgccgg aatgatgcat gcctgtggtc atgatggaca taccgccatt gggcttgggc
     8401 tggcgcatac ccttaaacaa ttcgagtccg gactacatgg cgtcatcaaa ctgatttttc
     8461 agcctgcaga ggaaggtacg cgtggcgcgc gggcgatggt cgatgcaggt gtcgtagatg
     8521 atgttgatta ttttactgcc gtgcacattg gcactggcgt acctgcgggc accgtggtgt
     8581 gcggcagtga taattttatg gcaaccacca aatttgacgc gcacttcacc ggtaccgccg
     8641 ctcacgcagg cgcaaaacca gaagacggtc acaatgcctt gttggcggca gcacaagcca
     8701 ctcttgcact gcatgcaatc gccccgcaca gcgaaggagc ttccagagta aacgtgggcg
     8761 ttatgcaggc aggaagcggt cgtaacgttg ttcctgcctc ggcgttgctg aaagtggaaa
     8821 cacgcggggc cagcgacgtc attaatcaat atgtttttga ccgtgcacaa caagcgattc
     8881 agggcgcagc aaccatgtat ggtgtcggcg ttgaaactcg tctgatgggt gcagctaccg
     8941 ccagttctcc ttcgccgcaa tgggtcgcat ggttgcaaag tcaggcggct caggtcgcgg
     9001 gggtcaatca ggccattgaa cgtgttgaag cgcctgcggg ttccgaagat gccacattaa
     9061 tgatggcccg cgtgcagcaa catcaagggc aagcctccta cgtggtgttt ggcacacagc
     9121 tggcggcagg tcatcacaac gaaaaattcg attttgacga gcaggttctc gctattgccg
     9181 tcgaaacgct ggcgcgcacc gcgctcaatt ttccctggac gcgaggtatc tgatgcagga
     9241 aatctatcgt tttatcgacg atgcgattga agccgatcgc caacgttata ccgatattgc
     9301 cgatcaaatc tgggatcatc cagaaacacg ttttgaagag ttctggtcag cggagcatct
     9361 ggcttcggct ctggaatctg caggcttcac cgttacccgc aacgtaggca atatcccaaa
     9421 tgcctttatt gcttcgtttg gtcaaggcaa accggttatc gccctgctgg gagaatatga
     9481 cgccctggca ggtttaagtc agcaagctgg ttgcgcgcaa cctacatccg cgacgcccgg
     9541 tgaaaatggt cacggttgcg gacacaattt gcggggaacc gccgcctttg ccgctgcaat
     9601 agccgtcaag aaatggctgg aacaatatgg gcaaggcggc acagtgcgct tttatggttg
     9661 tcctggcgaa gaaggcggct cgggtaaaac gttcatggtc cgcgaggggg tatttgatga
     9721 tgtggatgcg gcactcacct ggcacccgga agcctttgcc ggtatgttca atacccgcac
     9781 gctggcaaac attcaggcat catggcgctt taaagggatc gcagcacatg ccgcgaattc
     9841 ccctcatttg ggacgcagcg cccttgatgc cgtaacgttg atgaccactg gcaccaactt
     9901 cctcaacgaa catattattg aaaaagcgcg cgtacactat gccatcacaa atagcggcgg
     9961 gatctcgccc aacgtggtcc aggcgcaggc agaagtgctt tatcttatcc gcgcccccga
    10021 aatgaccgac gtgcagcata tttatgatcg ggtcgccaaa atcgccgaag gtgcggcatt
    10081 gatgaccgaa accacggttg aatgccgctt cgacaaagcc tgttccagtt atctcccgaa
    10141 tcgcacctta gaaaatgcca tgtaccatgc cctatcccat tttggtaccc cggaatggaa
    10201 ctccgaagaa ctggcttttg cgaaacaaat tcaggctacg ctcaccccca acgatcggca
    10261 aaacagtctg aataatatcg ccgcaaccgg tggcgaaaac ggcaaggctt ttgcactacg
    10321 tcatcgtgaa acggtactgg cgaatgaggt cgctccatat gccgccaccg ataacgtgct
    10381 tgcggcatcg actgatgtcg gcgacgttag ttggaaactg cctgttgccc agtgtttcag
    10441 cccctgtttt gccgtcggta caccgctaca tacgtggcaa ctggttagcc aggggcgaac
    10501 atctattgct cataaaggaa tgctgctggc ggcgaaaact atggcagcaa ccacagtcaa
    10561 tctcttcatt gattcagggc tattgcaaga atgccaacaa gagcatcagc aagtaacgga
    10621 cacgcaaccg tatcactgcc ctatcccgaa aaacgtgaca ccgtcacctt taaaataaca
    10681 acaacaacgc aaacacaaca accgaggaat gcccatgagt atgtcatcca taccgtcgtc
    10741 ctcccaatcc gggaagctct atggctgggt cgaaagaatt ggtaacaagg ttctccatcc
    10801 ttttttgctc tttatctatt tgattatcgt actcatggtg acgacggcaa ttttgtcggc
    10861 ctttggcgtc agtgcgaaaa acccgaccga tggaacgccg gtggtggtga aaaacctgct
    10921 cagtgtggaa ggattacact ggtctttacc caatgttatt aaaaacttta gcggttttgc
    10981 tccacttggt gcgatcctgg cgctggtttt aggtgccggt ctggcggagc gcgtcggctt
    11041 actgccagcg ctaatggtta aaatggcatc gcatgttaat gcccgctacg ccagttatat
    11101 ggtgctgttt attgcttttt tcagccacat ttcttccgat gcgacgttag tgatcatgcc
    11161 accgatgggt gcgctgattt ttctggcggt gggcaggcat ccagttgcag gtttactggc
    11221 tgccattgca ggcgtaggtt gcggctttac ggctaattta ctgattgtca caaccgacgt
    11281 gttgctgtcg gggatcagca cggaagcggc ggctgcgttc aatccgcaaa tgcacgtcag
    11341 tgtaattgat aactggtatt ttatggccag ctccgtagtc gtactgacga ttgttggcgg
    11401 cctgataacc gacaaaatca tcgagccacg gttaggtcaa tggcagggaa acagcgatga
    11461 gaaactgcag acattgaccg aaagtcagcg ttttggttta cgcatagcag gtgtcgtatc
    11521 gctacttttt attgctgcga ttgcgctgat ggtgatcccg gaaaacggga tattgcgcga
    11581 tccgattaat cacaccgtga tgccatcacc ctttattaaa ggtatcgtgc cactgatcat
    11641 tctttttttc tttgttgtct cgctggctta tggcatcgct acccgcacaa ttcgacgtca
    11701 ggcggattta ccgcagttaa tgattgaacc gatgaaagag atggcaggat ttatcgtgat
    11761 ggtttttccc ctcgcccagt ttgtcgccat gtttaactgg agcaacat
//
LOCUS       DAESHI010000126        11779 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.127, whole genome shotgun sequence.
ACCESSION   DAESHI010000126 DAESHI010000000
VERSION     DAESHI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.127"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..611)
                     /locus_tag="KW011_003536"
     CDS             complement(<1..611)
                     /locus_tag="KW011_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HBH7661787.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRR"
     gene            complement(734..2380)
                     /gene="fumB"
                     /locus_tag="KW011_003537"
     CDS             complement(734..2380)
                     /gene="fumB"
                     /locus_tag="KW011_003537"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBH7661788.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="KW011_003538"
     CDS             complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="KW011_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HBH7661789.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="KW011_003539"
     CDS             complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="KW011_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HBH7661790.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5085..6716)
                     /gene="dcuS"
                     /locus_tag="KW011_003540"
     CDS             complement(5085..6716)
                     /gene="dcuS"
                     /locus_tag="KW011_003540"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HBH7661791.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            6897..7127
                     /locus_tag="KW011_003541"
     CDS             6897..7127
                     /locus_tag="KW011_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBH7661792.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            7139..7411
                     /locus_tag="KW011_003542"
     CDS             7139..7411
                     /locus_tag="KW011_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBH7661793.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            7638..7934
                     /gene="ghoS"
                     /locus_tag="KW011_003543"
     CDS             7638..7934
                     /gene="ghoS"
                     /locus_tag="KW011_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBH7661794.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            7962..8135
                     /gene="ghoT"
                     /locus_tag="KW011_003544"
     CDS             7962..8135
                     /gene="ghoT"
                     /locus_tag="KW011_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HBH7661795.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="KW011_003545"
     CDS             complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="KW011_003545"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBH7661796.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(10008..11465)
                     /gene="dtpC"
                     /locus_tag="KW011_003546"
     CDS             complement(10008..11465)
                     /gene="dtpC"
                     /locus_tag="KW011_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HBH7661797.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(11524..>11779)
                     /locus_tag="KW011_003547"
     CDS             complement(11524..>11779)
                     /locus_tag="KW011_003547"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HBH7661798.1"
                     /translation="TEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEF
                     LQMLCEIGAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
BASE COUNT         3044 a         2858 c         2892 g         2985 t
ORIGIN      
        1 aagcgccgta gctggcggca atgaaagcga gcgaggaata tcaccgtcgt taatgcgcca
       61 agcagcgcgc agaacatatc ggattgcgtg tcccactggt cgccctgggt gccgagaaaa
      121 tcatccgctc cctggcccat tgccagcgcc gcccaccact cgattaattc atacatcgcg
      181 cttatcgcca gcgccacgca gcagactagg aacgccacca ttttacgtcc gcgcacgtac
      241 atcccgcgca cgagaatttc tcgtgccacc agtgcaggca ccagcccctg gaaaaaatgc
      301 cccagcttgt catacggatt acggctcaac cccagccatt cctgcacctc aaaaccgatg
      361 gggactttcg cgtaggtgta ttgcccgccg accatcagga tgatggcgtg aaagaaaatg
      421 agcgtataga gcagcggcgt taacggataa cgtttagcgg tggcaagcag tagcggcacg
      481 acaataatca ccggtgtcac ttccatcagc caagtgagtt tgtcatgggc cgaaatgccg
      541 gtataaatca ggattaacgt tagcgccagt gcgccggtgt taagaattaa cggtttgagt
      601 gtgcgggtca tggtgagttc gcaagtcagg gaaaatactg actattcacc agcaagcatg
      661 aaattacaat cgcaaaagag gtggcccagg gggatcacct ggcagcatgc tgccaggcgc
      721 tgggccgaag aggttactta gtgcagttcg cgcactgttt gttgacgatt tgctggaaga
      781 agtcgttacc tttgtcatcg accaggataa acgccgggaa atcttctact tcgattttcc
      841 agatagcttc catacccagc tccggataag cgacgcattc cagatgcttg atgctctgct
      901 gcgccagtac cgccgccgga ccgccgatgc taccgaggta gaagccgccg tgtttatgac
      961 acgcgtcggt aacctgctgg ctgcggttgc ctttcgccag catgatcatg ctgccgccgt
     1021 gggattgcag cagatccaca taggagtcca tacgacctgc ggtggttggg ccaagtgaac
     1081 ctgatggata accggcaggg gttttcgccg gacccgcgta gtagatcggg tgatctttga
     1141 tgtactgcgg aagttcttta ccggcgtcaa tcagctcttt cagcttggcg tgtgcaatat
     1201 ctcgacccac aataatggtg ccagtgagcg acaaacgagt ggaaaccgga tattgcgaaa
     1261 gctgggcgag gatctctttc atcgggcggt taaggtcgac cttcaccgct tcgccttcac
     1321 cggcctgacg cagttcttgt ggaatgtact gacctggatt gtgttccagt ttttcgatcc
     1381 agataccttc gcggttgatt ttcgctttaa tgttacggtc agcggagcag gagacgccca
     1441 tgcctaccgg gcaggatgcg ccgtgacgtg gcagacggat aacgcgaatg tcgtgcgcga
     1501 agtatttacc gccaaactgc gcgccaagac cgagtttctg ggcctcttcc agcagttcct
     1561 gttccagctg gacatcgcgg aacgcctgac catgttcgtt cccttccgtc ggcagttcat
     1621 cgtaatagtg agcgcttgct aatttgacgg ttttcaggtt ggtttccgca gacgtaccgc
     1681 caatcacaaa cgcgatatgg tacggcgggc aggctgcagt accgagggta cgcattttct
     1741 cgacgaggaa gtttttcagt ttgccgggag tcagcagggc tttggtttcc tggtagagat
     1801 acgttttgtt ggcagagccg ccgcctttcg caacgcaaag gaatttgtac tcatcgccat
     1861 ctaccgcgta caggtcgatt tgcgcaggca ggttagtgcc ggtgttgacc tctttgtaca
     1921 tgtccagcgc cgcgttttgc gagtagcgca ggttatcttc gatataggtg ttatagacgc
     1981 ctttcgacag cgcttcttca tcaccgccac cggtccacac gcgctggcct tttttaccga
     2041 cgatgatcgc ggtgccggta tcctggcagg tcggcagcac gcctttggcg gcgatttcgg
     2101 agtttcttaa gaattgcagt gccacgtact tgtcgttttc gctggcttct ggatcgtgaa
     2161 gaatagccgc aacctgtttc tggtgtgccg gacggagcat gaaagaagcg tcgtgaaagg
     2221 cttgctgcgc cagcagggtc agggcttctg gttccacttt caggatggtt tcgccgtcga
     2281 agtcggcaac gctaacgtaa tcggaagtga gtagatagta ttcggtattg tctttcccca
     2341 tcgggaaagg tgcctggtag ataaagggtt tgtttgacat agcttccagc ctgtaactct
     2401 gtatttgtta ttcgaaaatg gcgtgccgcg tagtcacggg cggcacgcaa agtgcattta
     2461 taagaacccg tacatcgcgg cgaagatcca gccgaagacg cacgatacgc tcacaccaat
     2521 caaccccggc agaataaagc tgtggttgat gacgaagcga ccgatgtggg tggtgccgga
     2581 acggtcaaac tgaatcgctg ccagatcgct cggataagtc ggcaggatgt aataaccgta
     2641 gcaagccggt gctgaagcca cgatgtatgc cggatcaacg ccgatcgcca gcgcgaccgg
     2701 aacaatcgcc gccagcgccg cagcctgaga gtttacaaac ttggaaacca gcagcagaac
     2761 aatggcatag gcccacggat actctttcac catttcaccc agtacgccct gaatttcaga
     2821 catatgcgca ccgaacatgg tttctgccat ccatgcgata ccgtacaccg ccacgatggc
     2881 gatcataccg gaacggaaga cttcgttttt tgagatagac gcgggattgg ttttggtcag
     2941 gataataatc agcgccccgg tcagcagcat aaacatctga ataaccagta ccatcgacag
     3001 cggtttgccg ccgaaggatg gacgcaggtc cgaatcagca ccaagaaggg cgactacagc
     3061 gattgccccg aggaaaatcc acattgccag ccagttgctt ttcggcagtt ttttgtccag
     3121 cagcgtcgcg gtatcaccgt aaacatactc acggttttcc ggtacggaga tgaatttctg
     3181 gaactcttcg tctttatcca gatctttacc acggaaccag ctgaagatac cgatcgccag
     3241 gataccgatt aacgtcgatg gaatggtgat tgccagcagg tcgaggaact caagatggcg
     3301 accatcaaag gtgacattac ccagcattgc aaccagagac acgaccgcaa ccgacaccgg
     3361 actggcgatg atccccatct gtgcaccgat agaacttgcc gccatcggac gttccggacg
     3421 gatgttgttc ttaatggcga cgtcgtagat gatcggcaga atggtgtaaa ccacatgacc
     3481 cgtaccgcaa agaatggtca gcgtacaggt cacaaacggc gcgacaattg agacatattt
     3541 cgggttgcgg cgcagcagct tctcggcaat ttgcagcatg acatcaagac cgcccgaagc
     3601 ttgcaaggtc gccgatgccg ccaccaccgc aatgataacc agcatgacat caaccggtgg
     3661 tttacctggc tgaaggtgga agacgaagac cagaatgacc agaccgatac cgcctaataa
     3721 acccagcgcg ataccaccct ttctggcacc ataaaacaga catatcagta ttatgataag
     3781 ttggatagta aataacatgt gtgaaccctc gcgataatcc tatttaaatt tttgctgaat
     3841 agatcacagt cacgttctgt tttgtatgaa ctgttttcag agcctgacta aatatccgtt
     3901 tggtcgttac cggctttagc aaatacctca cagtgaatat tggctgatta aagcagcggc
     3961 aaatttatgc actgtgataa gcggcttttt cagagagaaa gactctctgg cgatgggttt
     4021 atagtttgta ctgcgcagat gttaatgttt tgtttgctgt tatcttattg atatttatcg
     4081 gtctattttt tgttctcagg gcagatgtca ttaggtttat agattaatct gatctaccca
     4141 tttgtgggta aaaatacaca taatgcgggt gacataatag ttaattaact tttgttagcg
     4201 ttttgaaatt aaaaacaccg ttcacctgaa gagatattaa tttttagcga tgatgcaggg
     4261 ataattatat ttgatctggc acaagtttta ctgatgaagg atgtaacttg tgccaggggt
     4321 attttgcatt acggtaataa ttattactta cagataacca gcgatccgtt attggcaata
     4381 ttgtttcagt agtgagtagt gttctgcctg aatacggtaa cggtaaactg gacgccccgt
     4441 gacgccataa tggatactgg tgaacaagat gtggcagttg accagccaga tgaggtattt
     4501 acggcaggaa acacgcgaaa tgttaacctc gttggctagc tcgtcggttg aaaattcata
     4561 gtcctgatgc gcgtcaatcc actggcacag tgtgcgtaac gtctgcggcg ttaagccttt
     4621 tggcaagcga cgaggatcct gttcgttgga gctgctgccg tggattagct gatcaagctc
     4681 ggcctggtca taatactgat gtttttccag cgccattttc ttttgccgcc agtcggtgag
     4741 cgcctcttca aagcgggaag cctggaaggg tttgatcagg taatccacga caccgtaatg
     4801 cagcgaatct ttaatggttg ccgcatcggc tgcggaggag atgacaatca catcactttt
     4861 gcaacgcgcg ttatgcagga caggcagtaa atcgagcccg ttctcttttt gcatatagat
     4921 atcgagcaat atcaggtcga taggcgtatc gctattgaag ataatctctt tggctttctc
     4981 cagcgtcgag gctgttccac agcattgaaa gcctgggatt tgtgctacgt atcggcgatt
     5041 cagctccgcg accattgcgt cgtcatcgat aattaataca ttgatcatct gttcgacctc
     5101 tccccgtccc agggtatctg gacaaaaaat tgtgtgaaaa tcccgggttc cgattccacg
     5161 gcgatgctgc cgccgagatt ttctacctgt tgtttgacaa gtgctaaacc gacgcctcgc
     5221 tcgcttcctt ttgtcgagac acctttgtca aaaatgtgat cgattttgtc gggtgcgatc
     5281 cccggtccat catcgttaac ttcgcagtgc agccagccgt gacggtagtg caatgttacg
     5341 ctaatttcgc caccgggttc cggccctaat gcttccagcg cgttttctat cagatttccc
     5401 aatgtggtaa tcagcgtcgc gacctggtcc tcactgccgc tgtctggcag ctggctttca
     5461 ctgtttaaaa tcagcgtatg gcctaaatcg gtcgcgcgat taatcttgct gattaaaaaa
     5521 ccagcgataa ccggagattt gatcttaccc agcagagagc caatctcttc ctgatagtta
     5581 ttggctgttt ttagaatgta atcttccaac tgcttataac tcttcagatg caataatccg
     5641 agaatcacgt gcaatttatt cataaattcg tgggatcgtt cacgaagtgc gtcggcatag
     5701 ttgaccagac cgtcgagtcg ctgcatcagt ttacgtactt cggttttgtc cctgaaggtt
     5761 gaaatggcac cgatgataac gccattactg cgcaccggaa cggtgttgat cagtaatagc
     5821 cggtctttaa tcgtaatctc ttcgtcgcgg cgcggcgtac cgtcgcgtaa cacttccgag
     5881 acatctacca cctgtgacca tgagtggctg agcgtcgaca gtttctcatc gtcctgcgac
     5941 ttacggtaat tcagcaattc ttgtgcggca tcgttgatca gcgtgacctc gccgcgatcg
     6001 tccacggcaa cgacgccttc tttgatagac tgcaacatgg cctggcgttg ctcaaacagc
     6061 gtggagattt cgtagggttc caggccgaaa aggatttttt tcagtacctt aaccagaatg
     6121 caggtgccaa tcagtccgac cagcatgcca aataataccg accagataat gctccagcga
     6181 ctgtcattga tttgttgggt cacgcggctt aactcaaggc cgatcgccac cacgccaatt
     6241 tgtttatgat tttcatcgta gatgggggta aatacgcgta aagcctgcgc cagaaaaccg
     6301 cgattgatag cgacattttc tttgccattc agcgctttaa ggatgtcatc acctttaaat
     6361 ggctgaccaa tacgctgggc ttcaggatgc gagtagcgaa gactttgcat atcggtaacg
     6421 acaataaaca gcagatcgtt gcgtttgcgc acggcttccg cgatggcctg gatgccgctc
     6481 tcctgcggtt ttttctgcaa gccctgacgg atttccggcg agttggcgag ggtacgcgcc
     6541 actgccagtg ccttgttggc tagcccgtct cgcgtcatat cactgatttg cgagaagtaa
     6601 atcagatgca ccaccaatag caccgagaac agtaccgcac tgaccattaa gatcactgtg
     6661 gtactcaatt tcatcggacg tttgcgtaac atgcggtagg gcaatgaatg tctcatcagc
     6721 ttccttgtgt gacaaatttc ttaaacatta tctctgatga ggcgggtaat tcaaagggag
     6781 taagaatgat tggctatata ggggaagaga ttctggcaac ggaaactgcc agtgctgtat
     6841 gaagattcca gggctatgct tatagcgata atcatactga tgagagaggg aaagtcatgg
     6901 atcaggcgct actggacgag ggttatcgct gttataccgg cgaaaagatc gatgtctatt
     6961 tcaacactgc gatatgtcag cattctggca attgcgtacg tggcaacggc aagttattta
     7021 atctcaaacg aaagccgtgg atcatgccgg atgaagtcga cgtcgccact gtggttaaag
     7081 tgattgatac gtgcccgagc ggcgcgctga aataccgtca taaataagcg agggtaaaat
     7141 ggaaatacgc gaaggccaca ataaatttta cattaatgac gaacaaggca agcaaatcgc
     7201 tgaaattgtc tttgtgccga ctggagagaa tttagcgatt atcgaacata ccgatgtcga
     7261 tgaaagcctg aaagggcagg ggattggtaa acagctggtt gcaaaagtcg tggaaaaaat
     7321 gcgtcgggaa aaacgaaaaa ttatcccact atgcccattt gcgaaacacg aatttgataa
     7381 aacgcgggag tatgatgata ttcgcagttg atgggagagt acagagtcac gatatttttc
     7441 atgctctccg cggagtgatg caggagagca tctgaagggt agggggatgc acaaagaatg
     7501 ggcagagagc gcgttttttt gtcccaagtc atccccttta ctgagcaaaa aaaagaatat
     7561 ctcctatatg agaatcatca atcggggtta tcaagttttg cgtccccaga gcattcaata
     7621 ttgataggag tcatattatg gaaggtaaaa acaagttcaa tacttatgtt gtttcttttg
     7681 attatccatc atcttattcc tcagtgttct taaggttaag atcattgatg tatgatatga
     7741 atttctcttc tatcgtggct gatgaatatg ggataccacg acaattgaat gaaaattcct
     7801 tcgcaataac gacatcgtta gccgcaagtg aaatcgaaga tttaatcagg cttaaatgct
     7861 tagacttacc ggatattgat tttgacctca acattatgac agttgatgac tatttccgtc
     7921 agttttacaa gtagccagga aggataagaa gaacataaaa aatggcacta ttctctaaaa
     7981 tattaatttt ttatgtgatt ggtgtgaaca tatcctttgt cattatctgg tttatctcac
     8041 atgagaaaac acatattcgt ttacttagtg cattcctggt cggaataacc tggccaatga
     8101 gtctgcctgt ggcattactt ttttctctct tttaggagct gtagttgagt ggacccgctg
     8161 gttatcgtga aacgaagcgt ctctcctcaa aactggattt gcggaacgac gaacgaagca
     8221 gtcgcctgac tgcttcgttc attaaagtga aatttatttc tgtgggcgca tcgccgggaa
     8281 gagaataacg tcgcgaatag tatgactgtt tgtaaacagc ataatcattc ggtcgatacc
     8341 aatacccaga ccagcggttg gcggcagacc atattccagc gcagttacgt aatcttcgtc
     8401 atagaacatg gcttcgtcgt cacctgcagc tttagcgtta acctgttcct ggaaacgttc
     8461 agcctgatct tctgcgtcgt ttaattcgct aaaaccatta ccgatttcac gaccaccgat
     8521 gaagaattca aaacggtcgg tgatttccgg gttaacatca ttacggcgcg ccagcggtga
     8581 cacttctgcc ggatattccg taataaaggt tggctgaatc agatgtgctt ctgccacttc
     8641 atcaaagatc tccgtgataa tacgtcccag cccccagctt ttctctaccg taattccgat
     8701 agattcagct aatgctttag cagcatcaaa attatccagg tcggccatat cggtttctgg
     8761 acgatatttt ttgattgctt cgcgcatggt gagtttttca aacggtttgc cgaaatcaaa
     8821 cacatgctcg ccataagtga ctttagtggt acccagaacc tcttgtgcca gggtgcggaa
     8881 cagtgactct gtcagttcaa tcaaatcgtg gtaatccgca tacgccatgt agagttccat
     8941 cattgtgaac tcaggattat ggcgaacaga aataccttca ttacggaagt tacggttgat
     9001 ttcgaatacc cgttcaaaac cgcctacaac cagacgtttc agatacagct ccggcgcgat
     9061 acgcagatac atatctaaat ccagagcatt atgatgggta ataaacgggc gagcagatgc
     9121 cccacctgga attacctgca tcatcggggt ttctacttcc ataaagccgc gcgcgaccat
     9181 gaactgacgg atagcggcca gaatttttga acggacaaca aacgtttgac gggatttatc
     9241 gttagcgatg aggtccagat aacgttgacg ataacggact tcctgatcct gcagaccatg
     9301 gaatttatct ggtaaaggac gtagtgcttt agtcagcagg cgcaactcag tacagtgaat
     9361 ggaaagctca cccgtttgcg tcttaaacag cgtaccgcgg gcaccgataa tgtcacccag
     9421 atcccatttt ttaaactgat cgttataaac accttctggc aggctatctc ttgcaacgta
     9481 cagttgaata cggccaccga catcctgcaa cgttacaaag gaggctttcc ccatgatacg
     9541 acgggtcatc attcggccag caaccgagac ttcaatgttt aaggattcca gttcctggtt
     9601 attcttcgca tcaaactctt cgtgcaactg gtcagaggta tggtcgcggc gaaaatcatt
     9661 gggaaacgcc acaccttgct gacgtagtgc cgccagtttt tcgcggcgat ttctcagttc
     9721 atcgttaaaa tcaatagcct cattggctcc ccgtgtttct tgttcagaca ttttggttcc
     9781 tctaaatcca gctttcaatt tagcgtagat aaagagacag atcggtacac tgtattatct
     9841 gcctcgacta ttaataactc agtttatctt atcgaaacta acgcttacct caagttgata
     9901 tcactaataa ataaccatca aaatggttta aatgacttat taatctcgat ttactagtgt
     9961 gattaactga aagttaattt attcataacc aattgttttt acgacagtta atcgttgctc
    10021 tcctgtatca tattcgtcgg cgtgctaaac agaaaacggg tagcaaaggc caatacgacg
    10081 atgatcgcga cacaagccaa cgtccactct cccatctgag aaaagaatcg ctggtaagct
    10141 gcaattacca taccgctaat ttgcgactct gtcgtctgct gtgccacgac gcctgccagc
    10201 cagttggcga ccgcgcctgt tgccagcata taaataccgg ttaatacgcc agacattttc
    10261 agacgcgtga tttgcgcaat cgccaccggg tcaataaaga gttctgcaaa gcccattagc
    10321 gccaacccgg atatcatcac gcccattgac gcttgaccgt cagccgctgc atgtcgggca
    10381 tcaaatgcca acaacataaa gccacaagcc atcagcagta agccaaaggc aaacttcagc
    10441 cagacgcgca atgttgagtt gccgcggctt tctggcgacg ccagccaggc cagtacaacc
    10501 ccagcgagca tcactgcaat tgcattcacc gactggaata gtgctgtagg tacttcaata
    10561 ttgaatgcct gacgattcac aaagcgatcg ataaacaagc tgatggtact gccgccctgt
    10621 tgtgccagta cccagaacaa cgtcccgaca aacatcaaca atacaatttg ccaaagagca
    10681 cggcggtgtt cggggaattt gatcatcatg cgggcaatga tttgtgcggc aatgaggcaa
    10741 acgatcgcca gcaaatatcc ggaccagtcg ttctccagca gcagggtaaa aaatactggg
    10801 gctaaacaga gcatcaccac taaccagctc catactggta aggcaaattt gacgctagtg
    10861 agcgcttttt tatccatact acgcgtggac tggaaatgac gatgaccgct taagaaaatc
    10921 aacaaaccga taaacatgcc gccacccgca agggcaaagc caacatgcca tccataccac
    10981 tgagcagcca ggccgcaggc gattggggcg gcgatagaac cgatattgcc cgcagcatac
    11041 agcagcgaaa aaccgccatc acgtcgatga tcgttctcgt cgtagagctc gccaagcaaa
    11101 cagctgatgt ttgatttgaa taaaccgtag ccacaaataa tgattgccag cgccagatac
    11161 aggctaaagg ttgaatttgt atcaatgccc agcaccacat ggccaagggt cattaacagc
    11221 gcgccggcaa tcactgcagt gcggttgccg agcaggcggt cggcaagcca gccgccgaga
    11281 ataggggtaa cgtaaaccag agaagcatat gcgctgaaca ggctgatggc atggttatca
    11341 tcaaaaccaa gctgatgggt gagatagaga atgagtaagg cacgcatgcc gtaaaaactg
    11401 aagtactccc agatttggat cgccacgata tagtatatcg cgcgcggctg tgagggtgtt
    11461 ttcatgtgtt ctccttatga gcaaaaaagg gaagtggcaa gccacttccc ttgtacgagc
    11521 taattatttt ttgctttctt ctttcaatac cttaacggta tagcggccat cagcctgacg
    11581 gtatgcaccg tgaatatcgg tttcaaatcc cggatagtga gcgccgattt cacacagcat
    11641 ctgcaggaac tccagaaccg gacggctttc ttcggtgatc atttcacccg gcattaccag
    11701 aggaactccc ggcggatacg gaaggatcat attggcgtta atacgaccta ccatttcgtc
    11761 gaggtaaact tcttcggtc
//
LOCUS       DAESHI010000127        11719 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.128, whole genome shotgun sequence.
ACCESSION   DAESHI010000127 DAESHI010000000
VERSION     DAESHI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11719
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.128"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(270..1043)
                     /locus_tag="KW011_003548"
     CDS             complement(270..1043)
                     /locus_tag="KW011_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBH7661799.1"
                     /translation="MHDQPLLRMSLPSIPSFVLSGLLLLCLPFSSFACATTSHISFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            1229..1489
                     /gene="dinJ"
                     /locus_tag="KW011_003549"
     CDS             1229..1489
                     /gene="dinJ"
                     /locus_tag="KW011_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HBH7661800.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            1492..1770
                     /gene="yafQ"
                     /locus_tag="KW011_003550"
     CDS             1492..1770
                     /gene="yafQ"
                     /locus_tag="KW011_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HBH7661801.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            1926..2666
                     /locus_tag="KW011_003551"
     CDS             1926..2666
                     /locus_tag="KW011_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HBH7661802.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(2637..3404)
                     /locus_tag="KW011_003552"
     CDS             complement(2637..3404)
                     /locus_tag="KW011_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HBH7661803.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(3610..4188)
                     /gene="lpcA"
                     /locus_tag="KW011_003553"
     CDS             complement(3610..4188)
                     /gene="lpcA"
                     /locus_tag="KW011_003553"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HBH7661804.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            4428..6872
                     /gene="fadE"
                     /locus_tag="KW011_003554"
     CDS             4428..6872
                     /gene="fadE"
                     /locus_tag="KW011_003554"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HBH7661805.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(6915..7361)
                     /gene="ivy"
                     /locus_tag="KW011_003555"
     CDS             complement(6915..7361)
                     /gene="ivy"
                     /locus_tag="KW011_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HBH7661806.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            7542..8312
                     /gene="yafV"
                     /locus_tag="KW011_003556"
     CDS             7542..8312
                     /gene="yafV"
                     /locus_tag="KW011_003556"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HBH7661807.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            complement(8354..9490)
                     /locus_tag="KW011_003557"
     CDS             complement(8354..9490)
                     /locus_tag="KW011_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HBH7661808.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVLGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKE
                     PEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFS
                     GIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAESGLS"
     gene            complement(9959..10336)
                     /locus_tag="KW011_003558"
     CDS             complement(9959..10336)
                     /locus_tag="KW011_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661809.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
     gene            complement(10336..10614)
                     /locus_tag="KW011_003559"
     CDS             complement(10336..10614)
                     /locus_tag="KW011_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HBH7661810.1"
                     /translation="MGKPDVTDSNLKNIIDDLYRPNAKVGSGSTADAVRYELATGEKV
                     GGRGHVLKAQDYTRALQDWLDKNPTASPGDRAAAENVLQDLQNALGGK"
     gene            complement(10784..11719)
                     /locus_tag="KW011_003560"
                     /pseudo
     CDS             complement(10784..11719)
                     /locus_tag="KW011_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
BASE COUNT         2911 a         2893 c         2762 g         3153 t
ORIGIN      
        1 tttgttctcc ttaattatgt aaggagtatt tgatcatgca taagcattaa aaaacagttt
       61 caagtaatga ggaatatctc aaattttaaa cataaaatgc gaattattta gtacaaaaag
      121 tgtacttctg atgatctttc cctgggtgat ggaataacag gaaagcggct cgcaaaaacg
      181 cacagatttg taggcatgat aagacgcgtc agcgtcgcat caggcatctg cgcaccaccg
      241 ccggatgcgg cgtaaacgcc ttatccgtcc tacaaaatcg tctcttccgt caatatccgc
      301 cgcgcgccca aaaaatgatc ctgccagaaa ggttcagcta accgacttac ccgaatggtt
      361 tccccggtgc gcggtgactc aataaactga ccgtcgccca gatacacccc catatggtcg
      421 gcaatctcgc ggctgtggat atggaaaaac agcaaatctc cccggcgcag gtcgttattc
      481 gccacaatgg ttgcccgacg gtagtgatac atctcattgg ccgtacgcgg gagcttcgcc
      541 tcgaggatct tgttgtaggc ataaaaaact aacccactac aatcgaagcc ttgatcgggc
      601 cgcgtgccgc cccagacgta aggcttgcca agctgctgct ccagacggtg aatcgccact
      661 tcggtgatgt tatgcaggtg atcgctgctt aaaaagtggt tgttttccgc cagcgcctgc
      721 cagcgtctgt cactcgcctt gagcggcgca ggaaaccatt ctggatgctg ttttatctgc
      781 tcccggttgt gctggcgcag cgcccttctg ctttgggcat tgccttccac aatgtagctg
      841 gcctgttttt tcagatgggt ttggtactgt tttaataaac gcgcacggtt ttgcatccgc
      901 tggcgggcgg cgtagctgaa agagatatgt gatgtggtgg cgcaggcaaa tgaagaaaac
      961 ggcaagcaaa gcaacagtaa tcccgacaac acaaaagagg gaatcgacgg caaagacatt
     1021 ctcaataatg gctggtcatg cattgatgtt gcagtgtatc ctggcccgta cctgaacaaa
     1081 cttaataaac cattattata aaattaataa atatatttca gtttattatg taaaaacatc
     1141 cgtttattaa attactcatg gattgtcgct gttgctcatt tgagctacaa ttcaagctga
     1201 ataaatatac agcacaggag atcccccaat ggctgctaac gcgtttgttc gcgcccgaat
     1261 cgatgaagat ctgaagaatc aggcggcgga cgtactggcc gggatggggc tgaccatctc
     1321 tgacctggtt cgcataaccc tcacaaaggt cgcgcgtgaa aaggcattgc cgtttgattt
     1381 acgcgagcct aatcaattaa ccattcaatc aatcaaaaac agcgaagctg gcgttgatgt
     1441 tcataaggcc aaagacgctg atgatttatt tgataaatta ggaatttaaa tatgattcaa
     1501 agggatattg aatactcggg acaattttca aaggatgtaa aacttgcaca aaagcgtcat
     1561 aaggatatga ataaattgaa atatcttatg acgcttctta tcaataatgc tttaccgctt
     1621 ccagctgttt ataaagacca cccgctgcaa ggttcatgga aaggttatcg cgatgctcat
     1681 gtcgaaccgg actggatcct gatttacaaa cttaccgata aacttttacg atttgagaga
     1741 actggaactc acgcggcgct ctttgggtaa agcacgctgc cgatgaaatt tgtaaaccct
     1801 gcgccgacag gcttgcgtaa aactcttttg gtgataatgt gagccttcca aatccagact
     1861 attccgattt gggatctatt ttgccgtcct ggcgtgtgta accgttttat caaggaataa
     1921 gcagtatgcg taaaatcgca ttaattcttg cgatgttgtt gatcccgtgc gtttcgtttg
     1981 ccggtttgct gggcagcagt agctcgacca cgccagtgag caaagagtat aagcagcagt
     2041 tgatgggatc ccctgtctac atccagatct tcaaggaaga acgtacgctc gatctctacg
     2101 tcaaaatggg cgagcaatat cagctgctcg acagttataa aatctgtaaa tattccggcg
     2161 gcttagggcc aaaacagcgt cagggcgatt tcaaaagccc ggaagggttt tatagcgtcc
     2221 agcgtaatca gttaaaacca gacagccgtt actacaaagc gattaatatt ggtttcccca
     2281 atgcctatga ccgtgcgcat ggttatgaag ggaaatacct gatgatccac ggcgattgtg
     2341 tttccatcgg ctgctacgca atgaccaatc agggtattga tgagatcttc cagttcgtta
     2401 ctggtgcgct ggtgtttggt cagccgagcg tgcaagtgag tatttacccg ttccgcatga
     2461 ccgacgccaa tatgaagcgc cataaatatt ccaactttaa ggacttctgg gagcaactga
     2521 agccgggtta cgactacttt gagcaaaccc gtaagccacc gaccgtttct gtggtcaatg
     2581 gtcgttacgt ggtcagcaag ccgttaagcc acgaagttgt gcagccacaa ctggcatcaa
     2641 actacacgct ccccgaggca aaataagcgc cattcgcctg gcataatctt ttgccaggtt
     2701 tcattgcccg tcagcggctg tgttgcaatc accgtgacca catcattcgg tgtggtctgc
     2761 gagctgaagt cgatttccac atcctgatcc agcagcgttg ccacgccaaa cggcgcgcgg
     2821 cgggtgatcc agtgtaaatt agtcgagcaa tacgccatca catagcgccc gtcagaaagc
     2881 agcatgttga aaacgccctt ctgccgcagt tcatccgcca gtgaggcgat atatttaaac
     2941 actgccgcca tgttgcccgg tgtgcgcggg taacgctgcg ttaatttatg caggagccag
     3001 cagaaggctt tttcgctatc ggtttcaccg accgggcgga agttgccggt ttccagtgat
     3061 ttgtagcccg tcagttgtcc gttatgggca taagtccagt tacgccccca taactcacgg
     3121 gtaaacgggt gagtattttc cagcgccacc tcgccccgat tggcctggcg aatatgagcc
     3181 accaccgaac aggattttat cgggtagtcc tggacaagtt tggcgatggg ggaattaaag
     3241 ctgggttgtg gatctttaaa tgtgcgacag cctttacctt cgtaaaaggt aatgccccag
     3301 ccatctttat gtggcccggt tccaccacca cgctgcacca gaccggtgaa actaaagcag
     3361 atatcggtag ggacgttggc gctcatcccg agcaattcgc acatgggtaa cctccacaac
     3421 agcgggagcg catggccccc ggcgaagatt gatttaagaa ttttcaggcc ggatgcggcg
     3481 taaacgtctt attcggccta cggttcgcgt acagatttgt aggcatgata agacgcatca
     3541 gcgtcgcatc aggcatcagc gcacaagtgc cggatgcggc gtaaacgcct tatccggcct
     3601 acgccagact tacttaacca tctctttttc aattaactgg atcaggatat ggatcacttt
     3661 aatgtgaatc tcctgaatgc ggtcggcata accaaagtgc ggaacgcgaa tttcgatatc
     3721 cgccgtgcca gccattttac cgccgtcttt accggtcagg gtgatcactt tcatcccctt
     3781 ctcacgcgcc gctgcgatcg ctttgatcac gtttgcagag ttaccggagg tggagatccc
     3841 cagcagtaca tcgccttcgc gacctaccgc ttcaacgtag cgggagaaaa tatcattgaa
     3901 accgaaatca ttaccgacgc aggaaatatg actaacgtca gaaatagcaa tcgccgggta
     3961 gcccggacgg ttttcacggt agcgaccggt caactcttcg gcaaagtgca tagcgtcgca
     4021 atgggaaccg ccgttgccgc aggaaagcac tttgccaccg gctttaaagc tgtctgctaa
     4081 caggaccgcc gcgcgctgaa tggcgtgaat attggcgtca tcttttaaaa agttagccag
     4141 cgtttccgcc gcttcgttca gttcgttacg aataagatcc tggtacatga ggatatcctt
     4201 cagcataaat gtaatagaca aaatgcagtg taccggatac cgccaaaagc gagaagtacg
     4261 ggcaggtgct atgaccagga ctttttgacc tgaagtgcgg ataaaaacag caacaatgtg
     4321 agctttgttg taattatatt gtaaacacat tgctaaatgt ttttacatcc actacaacca
     4381 tatcatcaca agtggtcaga cctcctacaa gtaaggggct tttcgttatg atgattttga
     4441 gtattctcgc tacggttgtc ctgctcggcg cgttgttcta tcaccgcgtg agcttattta
     4501 tcagcagtct gattttgctc gcctggacag ccgccctcgg cgttgctggt ctgtggtcag
     4561 cgtgggtact ggtgcctctg gccattatcc tcgtgccatt taactttgcg cctatgcgta
     4621 agtcgatgat ttccgcgccg gtatttcgcg gtttccgtaa ggtgatgccg ccgatgtcgc
     4681 gcactgagaa agaagcgatt gatgcgggca ccacctggtg ggagggcgac ttgttccagg
     4741 gcaagccgga ctggaaaaag ctgcataact atccgcagcc gcgcctgacc gccgaagagc
     4801 aagcgtttct cgacggcccg gtagaagaag cctgccggat ggcgaatgat ttccagatta
     4861 ctcatgagct ggcggatctg ccgccggagt tgtgggcgta ccttaaagag catcgcttct
     4921 tcgcgatgat tatcaaaaaa gagtacggcg gactggagtt ctcagcttat gcccagtctc
     4981 gcgtgctgca aaaactctcc ggcgtgagcg ggatcctggc gattaccgtc ggcgtgccga
     5041 actcattagg cccgggcgag ctgctgcaac attacggcac tgacgagcag aaagatcact
     5101 atctgccgcg tctggcgcgt ggtcaggaga tcccttgctt tgcactgaca agcccggaag
     5161 cgggttccga tgcgggcgcg attccggaca ccgggattgt ctgcatgggc gaatggcagg
     5221 gccagcaggt gctggggatg cgtctgacct ggaacaaacg ctatattacg ctggcaccga
     5281 ttgcgactgt gcttgggctg gcgtttaaac tctccgaccc agaaaagctg ctgggtggcg
     5341 cagaagattt aggtattacc tgtgcgctga tcccgaccac cacgccgggc gtggaaattg
     5401 gtcgtcgcca cttcccgctg aacgtaccgt tccagaacgg accgacgcgc ggtaaagatg
     5461 tcttcgtacc gatcgattac atcatcggtg ggccgaaaat ggccgggcaa ggctggcgga
     5521 tgctggtgga gtgcctctcg gtaggccgcg gcatcaccct gccttccaac tcaaccggcg
     5581 gcgtgaaatc ggtagcgctg gcaacgggcg cgtatgctca cattcgccgt cagttcaaaa
     5641 tctctatcgg taagatggaa gggattgaag agccgctggc gcgtattgcc ggtaatgcct
     5701 acgtgatgga tgctgcggca tcgctgatta cctacggcat tatgctcggc gaaaaacctg
     5761 ccgtgctgtc ggctatcgtt aagtatcact gtacccaccg cgggcagcag tcgattattg
     5821 atgcgatgga tatcactggt ggcaaaggca ttatgctcgg gcaaagcaac ttcctggcgc
     5881 gtgcttacca gggcgcaccg attgccatca ccgttgaagg ggctaacatt ctgacccgca
     5941 gcatgatgat cttcggacaa ggagcgattc gttgccatcc gtacgtgctg gaagagatgg
     6001 aagcggcgaa gaacaatgac gtcaacgcgt tcgataaact gttgttcaaa catatcggtc
     6061 acgtcggtag caacaaagtt cgcagcttct ggctgggcct gacgcgcggt ttaaccagca
     6121 gcacgccaac cggcgatgcc actaaacgct actatcagca cctgaaccgc ctgagcgcca
     6181 acctcgccct gctttctgat gtctcgatgg cagtgctgga cggcagcctg aaacgtcgcg
     6241 agcgcatctc ggcccgtctg ggggatattt taagccagct ctacctcgcc tctgccgtgc
     6301 tgaagcgtta tgacgacgaa ggccgtaatg aagccgacct gccgctggtg cactggggcg
     6361 tacaagatgc gctgtatcag gctgaacagg cgatggatga tttactgcaa aacttcccga
     6421 accgcgtggt tgccgggctg ctgaatgtgg tgatcttccc gaccggacgt cattatctgg
     6481 caccttctga caagctggat cataaagtgg cgaagatttt acaagtgccg aacgccaccc
     6541 gttcccgcat tggtcgcggt cagtacctga cgccgagcga gcataatccg gttggcttgc
     6601 tggaagaggc gctggtggat gtgattgccg ccgacccaat tcatcagcgg atctgtaaag
     6661 agctgggtaa aaacctgccg tttacccgtc tggatgaact ggcgcacaac gcgctggcga
     6721 aggggctgat tgataaagat gaagccgcta ttctggtgaa agctgaagaa agccgtctgc
     6781 gcagtattaa cgttgatgac tttgatccgg aagagctggc gacgaagccg gtaaagttgc
     6841 cggagaaagt gcggaaagtt gaagccgcgt aattgaataa cggagccgaa aggctccgtt
     6901 tctttatccg ctaattattt aaaattaaag ccatccggat ggttttccag gctgccggtc
     6961 aacgccgcga acagcaccgt tttaccatca atcgaaagcg catcgttcac attcagccag
     7021 gtgagtttct cttgcgacgt tttctcatca atagtcgaga acagccccgt catctgatta
     7081 gatttctcgg accacatcac agcgatacgt tgcgagccac agtcatgcgg tttgcacgcg
     7141 ctcatcacct gatacgtatc atctcccaac gttacggttt gtgcgggagt ataagtaccg
     7201 cctttcatca cccaggcagg cagcttatgc ccttgtacca tctgattaaa tgcagctttg
     7261 gtggtttcgc cctttgcaag gctgctaatg gttaaatcat cctgcgccat tgcactggtg
     7321 gcgatgacca gagcggcgac tgtcgttatt gtcttaaaca tcattcctcc cgagcttatc
     7381 ctgcccatgt tattaacctt ctaaagcctg gcgataacac gataatttgc aaatcagata
     7441 tttccaatat cagcatttgt tatcaattta ttctgcgcta aggcaactgc ctgataaggt
     7501 gttaaagtta taaaaccctt cgcataaagg agttgtacac cgtgcctggt ttgaagatta
     7561 cgcttttgca gcaaccactg gtgtggatgg atggccctgc caacctgcgt cattttgatc
     7621 gtcaactgga aggtattacc gggcgcgatg tgatcgttct accgaagatg tttaccagcg
     7681 gctttgccat ggaagcggca gcttcgtcgc tagcacaaga tgacgtagtg gactggatga
     7741 cagctaaggc gcagcagtgc aatgcgctga ttgcaggcag tgttgcatta caaacggagt
     7801 ctggttcggt taaccgcttt ttgctggttg agccgggcgg cacggtacat ttttatgata
     7861 agcgtcatct gttccgcatg gcagatgagc atctacatta taaagcgggc aatgcgcgag
     7921 tgattgtgga atggcgcggc tggcgtattt tgccgttggt gtgctacgac ttacgttttc
     7981 cggtgtggtc gcgcaatctc aacgattatg acctcgccat ctacgttgcc aactggcctg
     8041 ctccgcgctc tctgcactgg caggcattgc tgacggcccg cgcgattgag aatcaggcgt
     8101 atgtggcggg atgcaatcgc gtcggcagcg atggcaacgg ctgccattat cgcggtgaca
     8161 gccgggtgat taatccacaa ggcgagatta tcgctactgc cgacgcgcat caggcaacgc
     8221 gcattgatgc ggagctgtcg atgatggcat tgcgggaata tagagaaaag tttccggcat
     8281 ggcaggatgc ggatgagttt aggttgcggt gagtgggggt tgtgtttaag gctgggggag
     8341 agtcggggta agactacgaa agcccgctct ccgcaaggac tgacgccaga tagtttctgt
     8401 ccatagctgc ttttcgcatc ttacgtctta accctgcctt gaatacctta tcattcgtca
     8461 aaatattaat agcgatgtgc cgtatccctg aaaataattc tgctgcattt cctcttctta
     8521 ttttgcagtc gtcttcattc attaccacgt ccagacgcca gtgcagctta ttctccacgt
     8581 gccagtggtt tcggattgct gtggcgaact tctctgcggt taaatcagca gaactgatat
     8641 aatatctgac cgtcatttct ggctctttct tttgttctgc tattatggac cgaaaggaga
     8701 ctgccacgca taatttcttc agccctttcc attcaaacgt gaaatcaata agttcatcag
     8761 ggacatcgca aacaatatga agacggattt cttctctgcc gtgactcttt tcactgattg
     8821 cgtaactgtc atgctctgga ttatttaatt ctttcagcgg aaatttttcc tcaaaggctt
     8881 tattaagccg cccctggttt ccttttaccg cgaataaata atcaccgccc tgtttttgta
     8941 tcttctctgc aatatctttc tggcatccca tcgcatcagt tgtgatgatt tttcctttaa
     9001 tatccagcat gttaagaagt tcaggaatgg ctgtaatctc attggatttc tcatccgtct
     9061 taatctgccc gagaaccaga ctgtgcattg ttgagaacgc actaatgaca tgaatcgctc
     9121 ccttgcggcg actcttgtca taagagtgcc ggagcgtttt tccatcaatt gcaatgacgt
     9181 ctttatcatc tgaagaatgg cagtcacgca tccagttaat aaagcactcg tgaaattttg
     9241 caggactgat acaggataca actctggcaa tagtatcgtg aacaggaata ccattttcaa
     9301 aatcaccata ttgcttcaaa aaatcgagat gtgtttcacc aaaatcctct atatcttccc
     9361 aaccctctgc accagaaatg acggcacaaa tagtcaacag tagaatatct gacaatttat
     9421 gttctacttt ccaggcttgt ctgtaatcgg ggataataga aatatgttcc atcagttttt
     9481 taagttccat tttgttctcc ttatttatgc aaggagtatt tgatcatata taagctatga
     9541 aaaatagttt taggtaataa ggaatatctc aattattgaa catttagcgc gaattattta
     9601 gtacaaaaag tgtacttccg atgatctttc cctgctccaa agtcttctaa aatcaacaaa
     9661 gtagactctt aattatattt atcaacacaa tagtctatcc tattactatc ttgacaagag
     9721 agaaccgttt tttatttaaa tatatgatac acatcattaa atatataacc atactttttc
     9781 ataaggcaat ctaacttagc tcttaaatca ccaaagccca tcacaccata acatctaata
     9841 gccattgcca cacgtcacta tggatttatt tccacctgca attacttcgt cctaacttta
     9901 atttaatctc aagaagcatt aagatctcaa tagcgagctt aatacttctt gaataatatc
     9961 actcctgacc aaaatttttc cacactaatg catgagactt tgatttgtca cccagaaatt
    10021 tacttaaatc ttgccaaagt aaagaattgc tatcggtcgc agtgactatt gcctctataa
    10081 gccctgtagc tacaactgtg gccaattcat catcaagcga gaccatacca tcttcgacat
    10141 gtttaaatat tttttctctt tcaatatccc ccatttcata aaaatttttt acaatcgttt
    10201 ttccaacctg agagaacaac ataaccaccg gtggcgtttc tggatgccag tattcattag
    10261 ctaatgagac tgtctcagac aaaacagtgg aaaatgatgc cattttttct atataactag
    10321 caatatattg gttcattact tgcctcccaa agcattctgt aaatcctgaa gaacgttttc
    10381 tgctgctgct cgatcccctg gagatgctgt tggattctta tccaaccaat cctgtaaagc
    10441 ccttgtataa tcctgtgctt tcaatacatg tcctctacca ccaacttttt cacctgtggc
    10501 caattcataa cgaacagcat ctgctgtgct tccacttcct acttttgcat ttggccgata
    10561 aagatcgtct ataatatttt tcaaatttga atctgtaacg tcgggttttc ccattttctg
    10621 tcgcaattca tctttatacc gttcatgctg catacgatcc cttgcgctat tctcatcttc
    10681 tggtccccat cctcccgggc ttcccgtact gccgccggcc aagcagttct tctcttcatc
    10741 cgtcaaatcc ttcccaacat tcggctgact ccctagggaa agattacgaa agcccgctcc
    10801 ccgcaaggac tgacgcgaga tagtttctat ccatggctgc ttttcgcatc ttacgtctta
    10861 accctgcctt gaatacctta tcattcgtta aaatattaat agcgatgtgc cgtatccctg
    10921 aaaataattc tgcggcgttt cctcttctta ttttgcagtc gtcttcattc attaccacgt
    10981 ccagacgcca gtgcagctta ttctccacgt gccagtggtt tcggattgct gtggcgaact
    11041 tttctgcggt taaatcagca gaactgatat aatatctgac cgtcatttct ggctctttct
    11101 tttgttctgc tattattgac cgaaaggaga ctgccacgca taatttcttc agtcctttcc
    11161 attcaaacgt gaaatcaata agttcatcag ggacatcgca aacaatatga agacggattt
    11221 cttctctgcc gtgactcttt tcactcattg cgtaactgtc atgctctgga ttatttaatt
    11281 ctttcagcgg aaatttttcc tcaaaggctt tatttagccg cccctggttt ccttttaccg
    11341 cgaataaata atcacctccc tgtttttgta tcttctctgc aatatctttc tggcaaccca
    11401 tcgcatcagt tgtgatgatt tttcctttaa tatctagcat gttaagaagt tctgggatag
    11461 ctgtaatctc attagatttc tcatccgtct tgatctgtcc gatgaccaga ctgtgcattg
    11521 ttgagaacgc actaatgaca tgaatcgctc ccctgcggcg actcttgtca taagagtgcc
    11581 ggagcgtttt tccatcaatt gcaatgacgt ctttatcatc tgaagaatgg cagtcacgca
    11641 ttcagttaat aaagcactcg tgaaattttg caggactgat acaggataca actctggcaa
    11701 tggtatcgtg aacaggaat
//
LOCUS       DAESHI010000128        11697 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.129, whole genome shotgun sequence.
ACCESSION   DAESHI010000128 DAESHI010000000
VERSION     DAESHI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11697
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.129"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(350..1603)
                     /locus_tag="KW011_003561"
     CDS             complement(350..1603)
                     /locus_tag="KW011_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH7661811.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1710..2603
                     /locus_tag="KW011_003562"
     CDS             1710..2603
                     /locus_tag="KW011_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH7661812.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            2738..3958
                     /gene="mlc"
                     /locus_tag="KW011_003563"
                     /gene_synonym="dgsA"
     CDS             2738..3958
                     /gene="mlc"
                     /locus_tag="KW011_003563"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HBH7661813.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            4083..4778
                     /gene="bioD"
                     /locus_tag="KW011_003564"
     CDS             4083..4778
                     /gene="bioD"
                     /locus_tag="KW011_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBH7661814.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="KW011_003565"
                     /pseudo
     CDS             complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="KW011_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
     gene            complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="KW011_003566"
     CDS             complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="KW011_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HBH7661815.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6839..7693)
                     /locus_tag="KW011_003567"
     CDS             complement(6839..7693)
                     /locus_tag="KW011_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBH7661816.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7695..8312)
                     /gene="dmsB"
                     /locus_tag="KW011_003568"
     CDS             complement(7695..8312)
                     /gene="dmsB"
                     /locus_tag="KW011_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBH7661817.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(8323..10746)
                     /locus_tag="KW011_003569"
     CDS             complement(8323..10746)
                     /locus_tag="KW011_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HBH7661818.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVEYDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITNIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPAYTPDFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(10807..>11697)
                     /locus_tag="KW011_003570"
     CDS             complement(10807..>11697)
                     /locus_tag="KW011_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH7661819.1"
                     /translation="ADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPI
                     YWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRK
                     DPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYAS
                     TFEGWDSPERSTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIAT
                     GDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLR
                     PSPLAKGNPQHTNLVEIEKI"
BASE COUNT         2958 a         2963 c         3020 g         2756 t
ORIGIN      
        1 cagacttccg tgagtggttg gtttcagcgt gtaacgatat gtacaaacgc tgaataaatt
       61 acagcattga taataacgta ttgtgactta agggaaattt agctaccaat aatagtagtc
      121 ttgatcgggt taactatttt actacttaca aagcggtaga ataactgcgc atcagtaata
      181 aatgacacac agcaaaatga atccgtttat ttgggtactt ataatcctga tgacgctaga
      241 cgcgctgcgg gaattggctg gcgcttcgtc tattttagga tggctattaa cgttggtttg
      301 agcaagcaat aagtccggac gtgtagttac cgcagtccgg gcttattttt caggcgtgca
      361 gacgacgatg caaacgcgtc ccgaccagca gagcaatgac cagcatcaga gcaataaatg
      421 cgccgacgcc gttccagcca tagttatgcc agaaaacacc acccagcgtc ccggcaatac
      481 tcgaccccag atagtaactg aacagataca gcgaggaggc ctggccttta gcgcgttttg
      541 cgcgggggcc aatccagctg ctggcaactg agtgggctgc gaagaatcct gctgagaaga
      601 gtaacattcc ggcaaagatc agccacagcg agctgaataa ggtcatcagt aaaccaaaca
      661 gcataacccc cgtcgaaaac aacatcaccg gaccgcgccc gtagcgggtg gtcatggttc
      721 cggctttggg tgagctccat gtaccggtca aataagccag cgataataag ccaaccacgg
      781 cctgactgac atgccagggg gaaagcatca accgatagcc gatgtaatta aacagcgtga
      841 cgaacgaccc catcagcaaa aagccttctg cgaacaataa cggtaatccc cggtcacgcc
      901 agtgcagacg aaagttgata aataacgtct tagggcgcag cgaagtcggg cgaaaatggc
      961 gtgattccgg aaggattttc cagaacatca acgccgaggc tagcgcgaaa caaccgattg
     1021 ccgccagagc aattcgccag ttgaaaaagt ccgtgaagac accactaatt aagcgtccgc
     1081 tcatgccgcc aattgagttg ccgctgatat acaaccccat cgaaaaggcc acgaaactgg
     1141 ggtggatttc ctcgctaaga taagtcatgc caacagctgc cacgccactt aacgaaagcc
     1201 caatcaaggc gcgcataatc aaaatgccgt gccagctggt catcattgtc gaaagtaacg
     1261 tacaaatgga ggccaacagt agcgccgtga ccatcactgg tttgcgacca atggcatcgg
     1321 atagggggcc agtaaacagc aaaccaatag ccaacatcgc cgtggaaatg gacagtgaaa
     1381 tactactgtt cgcgggggtt aagccaaact cctgcgaaag caccggaagg ataggctgca
     1441 cacaatagag gagtgcaaag gttgccagtc cggcagagaa cagcgccagg gtgacgcgca
     1501 taaattgcgg cgtaccgcgt ttaataaatt gatttggctg agaaatgctt tgcttgtcag
     1561 tgtcgcttgc cggagcgcca tcaacagttg tagtacggct cacttgaaat ccttgctaaa
     1621 tatgcctgtg gatcaggctt atacataggt taggaaaatc gaattgttct gtctaatata
     1681 ttaataatct caaataagat gttttaaata tgaatattga acttcgtcat ctgcgttact
     1741 ttgttgctgt tgcggaagag ctgcatttcg ggcgcgccgc tgcccgcctg aatatttcgc
     1801 aaccaccgct aagtcagcag attcaggcac tggagcaaca aattggtgcc cgactgctgg
     1861 cacgaactaa tcgcagtgta ttgctgacgg cagcaggaaa acagtttctt gcagatagtc
     1921 ggcaaatcct gtctatggta gatgacgctg ccgctcgcgc tgaaagactg catcagggtg
     1981 aagcggggga gttgcgcatt ggttttactt cgtccgctcc ttttattcgg gcggtgtccg
     2041 atacgttatc gctgtttcgc cgtgattatc ccgatgtcca tttacaaacc cgcgaaatga
     2101 acactcgtga gcaaatcgct ccgctcattg aaggaacgct ggatatggga ttgctgcgta
     2161 acacagcgtt accggagacg cttgaacacg cagtcatcgt ccatgaaccg cttatggcga
     2221 tgatcccgca cgatcatccc ctggcaaata acccgaatgt aacgctggct gaactggcga
     2281 aagaaccctt cgtctttttt gatccgcacg tcgggacagg gctgtatgac gatattctcg
     2341 ggctgatgcg acgttaccat ttgacgcccg tcatcactca ggaggtgggc gaggcaatga
     2401 ccatcatcgg tctggtttcc gccggtctgg gtgtttcaat tttgcctgct tcatttaaac
     2461 gtgttcagct caacgaaatg cgctgggtgc cgattgctga agaggatgcg gtttctgaaa
     2521 tgtggttggt ctggccgaaa catcatgaac aaagtccagc ttcgcgtaac tttcgtattc
     2581 atctgctgaa tgctctcagg tgagggaaat ttcagcgaaa aagcccgaaa aatgtgctgt
     2641 taatcacatg cctaagtaaa aatttgacga cacgtattga agtgcttcac catagcctac
     2701 agattatttc ggagcgcgaa aatataggga gtatgcggtg gttgctgaaa accagcttgg
     2761 gcacattgat caaataaagc agaccaacgc gggcgcggtt tatcgcctga ttgatcagct
     2821 tggtccagtc tcgcgtatcg atctttcccg tctggcgcag ctggctcctg ccagtatcac
     2881 taaaattgtc cgtgagatgc tcgaagcaca cctggtgcaa gagctggaaa tcaaagaagc
     2941 ggggaaccgt ggccgtccgg cggtggggct ggtggttgaa actgaagcct ggcactatct
     3001 ttctctgcgc attagtcgcg gggagatttt ccttgctctg cgcgatctga gcagcaaact
     3061 ggtggtggaa gagtcgcagg aactggcgtt aaaagatgac ttgccattgc tggatcgtat
     3121 catttcccat atcgatcagt tttttatccg ccaccagaaa aaacttgagc gtctaacttc
     3181 gattgccata accctgccgg gaattattga tacggaaaat ggtattgtac atcgcatgcc
     3241 gttctacgag gatgtaaaag agatgccgct cggcgaggcg ctggagcagc ataccggcgt
     3301 accggtttat attcagcatg atatcagcgc atggacgatg gcagaggcct tgtttggtgc
     3361 ctcacgcggg gcgcgcgatg tgattcaggt ggttatcgat cacaacgtgg gggcgggcgt
     3421 cattaccgat ggtcatctgc tacacgcagg cagcagtagt ctcgtggaaa taggccacac
     3481 acaggtcgac ccatatggta aacgctgtta ttgcgggaac cacggctgcc tcgaaaccat
     3541 cgccagtgtg gacagtattc ttgagctggc acagctgcgt ctcaatcaat ccatgagctc
     3601 gatgttacat gggcaaccgt taaccgtgga ctcattgtgt caggcggcat tgcgcggcga
     3661 tctactggca aaagacatca ttaccggggt gggcgcgcat gtcgggcgca ttcttgccat
     3721 catggtgaat ttatttaacc cacaaaaaat actgattggc tcaccgttaa gtaaagcggc
     3781 agatatcctc ttccctgtca tctcggacag catacgtcag caggcccttc ctgcgtatag
     3841 tcagcacatt agcgttgaga gtactcagtt ttctaaccag ggcacgatgg caggcgctgc
     3901 gctggtaaaa gacgcgatgt ataacggttc tttgttgatt cgtctgttgc agggttaaca
     3961 ttttttaact gttctaccaa aatttgcgct atctcaattt gggccaggaa agcataactt
     4021 agactttcaa ggttaattat tttcctagtt tatatttgtg aagcataacg gtggagttag
     4081 tgatgctgga gcgtttcttt attaccggta cagacacttc tgtagggaaa acggtggttt
     4141 cccgcgcatt gctacaagcg ttagcctccc agggaaaaac agttgcggga tataaacccg
     4201 tagcgaaggg gagcaaagag acacccgaag ggctgcgtaa taaagatgcc ctggtgttgc
     4261 agagtgtttc aaccatcgaa ctgccttatg aagcagttaa tcctatcgcg ttaagcgaag
     4321 aagaaagtag cgtggcgcac agttgcccaa tcaattacac cctcatttca aacggcctgg
     4381 caaacctgac cgataaagtc gatcatgtcg tggtagaagg gactggcggc tggcgcagtc
     4441 tgatgaatga tttgcgtcca ctctctgaat gggtggtgca ggaacagttg ccggtgttga
     4501 tggttgtcgg tattcaggaa ggttgcatta accatgcact gctaacagct caggcgattg
     4561 ccaacgacgg gctgccgctc attggctggg tggctaaccg aatcaaccca ggactggcgc
     4621 attatgcgga agtcattgat gtgctgggta aaaaacttcc ggcaccgctc attggtgaac
     4681 tgccttatct gccgcgcgcc gaacagcgag aactggggca atacatccgc ttagctatgc
     4741 tgcgcagtgt gctggcggta gatagagtca cggtgtaacg tccgcgaaat taccgacgca
     4801 atgacgcagg caatcaataa accggggagt agctgatact ccccggtcat ttcacatatc
     4861 atcaacgtcg acataatcgg cgcgtgcgtg gttgccgcca acagtgtcgc catcccggtc
     4921 aatccgagta aaagcgtaat ttcttcgcca tcagggaacc ataatcccag gctacgacca
     4981 tacaacatgc caatggcaag accgataaat agcgtcggtg taaagacacc accgggtgcg
     5041 ccggaaccgc tactcgccag cacggcacac agtttacaaa ggaagatccc ggcaatgatc
     5101 attaacagtg gtggggcggt taaaaaggat tgtacggtgc tatagccgtt gccccacact
     5161 gcaggtgtga acagggaaag cagacccacg atcaacccgc ctagtgccag ttgctagggc
     5221 ggcgcaagtt tgagactcac aaatccacga tgacaggcgt tcattaacgt taacaacagt
     5281 ggtccacaca gacctgccag cacacctgta ctgataatca gcgcatagtc actagcctga
     5341 accgttactg agagttgtac gctgtagagt aacgcgtcgc tatgattaat aagattgcta
     5401 accagcaacg ccacgacggc ggaaataatc accgggccga gagaggccaa catcatagtg
     5461 ccaaacagca cttcggctat aaataaactg ccagcaagcg gggcgcgata ggccgcagcc
     5521 attcccgccg cggccccaca ggcgatccat aatttccatt cctggcgtgg cgtaaaacgt
     5581 tgggcaaaac aggaggcggc aagggcagct aaaagaatca tcgcaccttc gcgaccaatt
     5641 gcactgccgc tggttactac cagcagcgag gcaagcgatt taaccaggct tgctgcgtaa
     5701 tcgaactgtc catcggtttg caacgcttcc atgtaatcgg tcggcgcatg agggcgttgt
     5761 tgggtaaatt tctgccagcc catcagcaac aaacccgccg ccagtccgcc gagcgccgga
     5821 gttagcagcc gtcgccaggg ggaaaggttt gtcgctgcat tgaccagact gccggagtca
     5881 ttattgagga acaaccactc cagtagcagc atcgcatgac gaaacccggc aacggcaaag
     5941 gccgcgagaa taccgacgac tgttgcgata agcagacggc ggaacatagt gcgcagatcc
     6001 ggatagatgt gtagatgatg catgagcaga aatgggcaat gaaacacggg cgactatttt
     6061 gcggcaaaag aggccaaccg caaaagcagt gcgtgcttta agagataaaa aaacgtgaca
     6121 ttgtaactta ttattgccga atcgcgcgta atcgtcacca tccggcaata ttacggtgat
     6181 cctatcgaaa gagtggttta accgcgacag gaatcaacag ttgtgattgc cattgcgcca
     6241 gcgttaagcg agccagttca cccagtgcac ggtagaaagg gtgttctgct ttttcgataa
     6301 aaacatcaag aaaacgtgtt gaccacggaa aaaggtgcca tgccagcaat tcttcgcatt
     6361 ctgtctggcg accattctcc gccaaccacg cagccatcaa cagcagtgaa ccaaaatgat
     6421 cttccggttc gttttgcttc atttcaaact gaatgccttt ctcgcgcatc cactgacgaa
     6481 gtgccaatgt tgaatcgcca aacagcacag attcgcgatc cagccagacc gatccccacg
     6541 gcggagacgg cagtgcccac gggccgacaa acaaacgctg ccaggcctgg gcgtgagtct
     6601 cttcacactg tgtctgaaaa gccgtcacca gcggcgctaa tgacgcttct ggtagaggcc
     6661 actgagtttc ccagccgtca ctggtgagta ccgcaaccag aggtgcggct tccgcactct
     6721 ctggagcgta ataaaacagc gcgcccaaca cgcgcgccgc gacagaaaaa ttatcttgct
     6781 gtgaaaaatg ggtcattcct aacatcctga aatgtgcggg ttgccccgca ccaatttgtt
     6841 aacctgcaat agccattcct actgtcatgt gcaggccata aaagagcaca cgcccgatca
     6901 tctcaccaac aagtatgagg attagcccca gaacaagtcc ggcaacgtga ggttcacggc
     6961 gacgaatgag cggacaaagc cagcatccaa gacctgcgca taacaatacc acacgccaga
     7021 cctgtaagga ggcgtaatct ggtaccagtg cgctggcttg ttgcacggat gagtgaatag
     7081 acgccagaga caaaccttgc agcacaatca ccccggcaca agcgatcagc gcgagaacgc
     7141 taatgatggc aaatggtgtg gtattaaatg taacgcgggc tgcccgcaga attgcggcag
     7201 caagtattgg accgctcaac aataccgtca ggaagaaagc cagcgtagtg taaccgttat
     7261 gccaggttgg cacagtgtcg atttgataca cgcaggtcat catccagacg aaaatgacgc
     7321 cgagcgccat gctgacaagt aaccagagtt tccccaacgc ttgcggcatt ttaccgatga
     7381 ccgccaccag ccaccatagt ccgccaacgg caaaaaatat ggaaccggca gcaatctcgt
     7441 tactcagacc agaagctccg attcgattca gcgagttaaa cgcccgcagt ggcgatccca
     7501 ggtgcatgac agaggccata aacccgatgc ccatcaacag ccagaggaaa aacatgccac
     7561 ggacaatacg ctgacggtct gcgtcatttt tggcggcgaa ccagccaatt ccgctaacaa
     7621 tcagcgcgcc tactacgcat tgacccaata cggtaaagat aaccagtggc cactcatgcc
     7681 atccatttcc cattttacac ttcctccgga ttagccagat aaccagtggt atcgctggtc
     7741 gggcggctgt tggcgttagg tttgataacg atattgggtt ttgtgaaatg cgcgcgcggc
     7801 agtggcgcga cggcagccag agtgccgtgc ttctggcgca gctcttcaat tggaccgaac
     7861 tcaagtgcac gcagcgggca ggattcgaca catatgggtt gtttcccctc ggcgacgcgc
     7921 gaataacaac catcgcactt cgtcatgtgc cctttttcag cattgtactg tggcgcgccg
     7981 tacggacaag ccatgtggca gtagcggcag ccgatacaaa catcttcatc aaccaccaca
     8041 aagccatctt cacgcttatg cattgcgccg ctggggcaga cttttgtaca tgcggggtca
     8101 tcgcaatggt tacaggagat ggagagataa taagcgaaca cgttctggtg ccagacgcca
     8161 ttatcttcct gccagtcgcc gccagcatat tcataaatac ggcggaaact gacttccggg
     8221 ccaagatctt taaaatcttt gcacgccagt tcgcaggttt tacagccagt acagcggctg
     8281 gaatcgataa aaaatccata ttgtgtggtc acgggctact ccttaaacct tttcgatctg
     8341 gacaagattg ctgtgcgacg ggtttccctt tgccagcggt gaagggcggt gagaggtcag
     8401 aatattgata ctgccgccgt gatcgacccg gtcaccaaac atatccgctt taagccaagc
     8461 accttgcccg atggcggtaa cgccaggcag aatacgcgga gtcacttttg cggcaatcag
     8521 catttctcca ttattgttaa atacccgcac ggtatcgcca tgacggatac cgcgtgcctg
     8581 agcatcaatg gggttgatcc acacctcttg tgggcaggcc tgctgtaaca catcaatatt
     8641 gccgtagctg gagtgggtac gcgctttgta atggaagccc gttaactgca gtggataggt
     8701 tttccgcagg ggatcgtccc agccatcaaa atctggggta tacgcaggaa ggggatgaat
     8761 aacttcatct tttttcaatt cccaggtatc cgcaatcttc gccagtcgtt cagaataaat
     8821 ttcgattttc cccgaaggtg ttttcaacgg gtttgcctgt ggatcttcac ggaatgcgcg
     8881 gaaagcgacg tagtgttctt ccgggcattt tttcttaaag atcccggtcg ttttcatctc
     8941 ctcgtagtcg ggcatctcag ggttacgttc cttcgttttc gcatggagat atttgatcca
     9001 ttcatgctga ctgcgacctt cagtaaaggt ttgataaacg tctggtccta agcgtttggc
     9061 gacttcactc agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg
     9121 ggcgaggatc acatagccca tattccctgc agattcatga gagataaggt cttcttgctc
     9181 tgttggcatc aggtcgggca acaggatatc gcaatactta gccgaggccg tcatgaagtg
     9241 gtcaatgcca acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt
     9301 gatgtcgcca tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg
     9361 gacatccagt ttttcttttc cacgaacacc atcacgggtc gcggtcattt ccgtaccatg
     9421 gtcgatggca tcagtccatg taaagacgga aatctgcgtt ttaacaggat tctcgagcat
     9481 cgggaaccat tctaccccca gatcccagct accttcgcgt acgcctgagt tgccgccgtt
     9541 tatgccgacg ttaccggtga gaacggaaag catggcaata gcgcgggatg tttgttcgcc
     9601 gttggagtgt cgttgtggcc cccaaccctg acaaatataa gcaggttttg ctgaaccgat
     9661 ctctcgtgcc aactggataa ttttttctgc cgggatgttg gtgatttttg ctgcccattc
     9721 cggcgtttta gctatgccgt caggcccttc gcccagaata taggctttat aatgcgcgtt
     9781 acgtggtgcg ttggcgggca gcgttttttc atcgtaacca acacaatatt tgtcgagaaa
     9841 tggctgatcg accatgtttt cagtaatcag tacccaggca atcgcacagg ccagtgcgcc
     9901 atcggtgcca gggcgaatgg gcagccattc atcttcacgc ccggcagctg tgtcgttata
     9961 acgtggatcg atgacgatca tgcgtgcgtt tgaacgttcg cgggcttgct cgacgtagta
    10021 agtgacacca ccaccgctca tccgcgtttc tgccgggtta tttccgaaca taacgaccag
    10081 tttcgtattg gcgatatcat ccgggctgtt gccatcattg gcaccgaaca tataactcat
    10141 tgcggcactg atctgtgcgg tactgtagct gccatagcga ctgagaaaac caccgcaaga
    10201 gttcatcaga cggtacggga cgtttgagtt ggtgatgttt ccgccatcta cacctgttcc
    10261 gtacaggaca tgtacagctt cattgccgta atcttttagg atccgccgaa gattatcact
    10321 gatggtatcc agggcttcgt cccaacttat ccgttcaaat ttaccttcac cgcgcttgcc
    10381 gacgcgcttc atgggatatt tcaacctatc aggatgattc atccgtcggc ggatagagcg
    10441 cccgcgtaaa cacgcgcgaa cctgatgatt accgtagacg tcgtcacctg tcgtatcata
    10501 ctccacccag tacacggtgt catctttcac atgcaaacgt aacagacagc ggctcccgca
    10561 gttaacggtg caggaactcc agaccgcttt ctcttctacc ggagcctctg ccgcccgaac
    10621 catttgggaa aatggcagag tgaaagcact gcttgccagc gcaaggctgc caagtgcgga
    10681 ggttttcatc agacttctac ggctgatttc agccttcatg agcgcctctg tggtatggat
    10741 tttcatcatt actcacttat tgcttttcaa acaaaatgtc atgccagaat ttatggttgt
    10801 agtgggttat attttttcga tctcgaccag attagtgtgc tgcgggtttc ccttcgccag
    10861 tggtgaaggg cgcagagtgg ttagcgtatt cacacagccg ccatggtcga ttttatcgcc
    10921 agacatattg gcctcgtgcc aggctccctg gcccatagcg ctaaccccag ggagaatgcg
    10981 tggtgttact ttggctggta gccgaacttc gccacgatgg ttaaacaccc gcaccatatc
    11041 gccggtggca atcccacgtt tctgtgcatc tatagggttg atccacactt cctgacggca
    11101 ggctgccttc aggacatcaa tgttgccgta ggtcgagtga gtacgggatt tgtaatggaa
    11161 accaaacagt tgcagtggga aggtactacg ttcaggggag tcccagcctt caaaggttga
    11221 ggcataaact ggcagtgggc ttatcacttc atctttttcc agttcccagg tacgggcaat
    11281 ttccgccagc ttgctggaat aaatttcaat cttaccggaa ggcgttttaa gtggatttgc
    11341 ctcggggtcg tcacgaaatg ctttgtaggc gacaaaatga ccattgggat ctttacgctt
    11401 atagataccc atttttttca gttcgtcgta agacggtaac gccggatctt tggcaagcat
    11461 tttggcgtac agatgttgta accattgttc ctgcgtgcga ccttcagtga acttttgata
    11521 gacgtcaggt ccaagacgtt tcgcgacttc actcaggatc cagtaaatcg gtttgcgttc
    11581 gaatttttcg ctggtgacag gctggaggaa aatgagatat cccatgttac cggcgtagtc
    11641 gttaggaata atatcttcct gctcaacggt catcaggtct ggcagcagaa tgtcggc
//
LOCUS       DAESHI010000129        11542 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.131, whole genome shotgun sequence.
ACCESSION   DAESHI010000129 DAESHI010000000
VERSION     DAESHI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.131"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            460..1386
                     /locus_tag="KW011_003571"
     CDS             460..1386
                     /locus_tag="KW011_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH7661820.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFERAEQTPGIKADWLV
                     RMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQARTLPDMLSSDAG
                     PLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(1336..2529)
                     /locus_tag="KW011_003572"
     CDS             complement(1336..2529)
                     /locus_tag="KW011_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH7661821.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2665..4389
                     /gene="iucA"
                     /locus_tag="KW011_003573"
     CDS             2665..4389
                     /gene="iucA"
                     /locus_tag="KW011_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HBH7661822.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            4390..5337
                     /gene="iucB"
                     /locus_tag="KW011_003574"
     CDS             4390..5337
                     /gene="iucB"
                     /locus_tag="KW011_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HBH7661823.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            5337..7079
                     /gene="iucC"
                     /locus_tag="KW011_003575"
     CDS             5337..7079
                     /gene="iucC"
                     /locus_tag="KW011_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HBH7661824.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            7076..8413
                     /gene="iucD"
                     /locus_tag="KW011_003576"
     CDS             7076..8413
                     /gene="iucD"
                     /locus_tag="KW011_003576"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HBH7661825.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            8419..10614
                     /gene="iutA"
                     /locus_tag="KW011_003577"
     CDS             8419..10614
                     /gene="iutA"
                     /locus_tag="KW011_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HBH7661826.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(11165..11308)
                     /locus_tag="KW011_003578"
     CDS             complement(11165..11308)
                     /locus_tag="KW011_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661827.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
BASE COUNT         2926 a         2896 c         2950 g         2770 t
ORIGIN      
        1 ctcaacgaag gggccgacaa acacgttgtc cctgagttca cattcataaa tgttgaccgg
       61 tttgacaata cggacattga tcccataaat cacatcccgg acagcacatt ccagaaattt
      121 catactcaca cgtcctttaa atacagatga catttgccgg aattataccg cccagattca
      181 cttgccatgt ttcagaatag cttataagcc gcacttttac cgatactcag caacaaatca
      241 ggtctgtatc cacttcagcc ggaaaccgga tctgcgcggc ggggaacggg caagaaatat
      301 gataattaag caacacacat aaaatcatat tgcataatcg catctgaatc gaacacttat
      361 tcgcatcact atcgatcatc acttcgcatc tcataccctt acccacaagt tgaaataata
      421 gtattatatt gaaaagacag agattatttc agaggtgtta tggaaaactg ggttattcag
      481 gagctaaaat cactggatat aggggactca agacttgata aaagagtaaa acatatcctc
      541 aatagcctca gtcgttcacc agaagaaagt attcctgttt cctgccgtac ctggagtgaa
      601 acaaaggcag cttatcgttg tttttcaaac gataaagtct ctgctaataa aattatggct
      661 cctcacaaag aaaatatcac tgaaagagct cagcagttta aaagagtttt agttttacag
      721 gatacaacag aacttaatta ttctggtcag aaagaaaaac aaggagttgg tcacaaaaaa
      781 cataaagatg aaagaaactt atttcttcat cctcaactcg tcatcagtga gtcaggtgtt
      841 tgcctcggtg tttatgatga ctatcagtgg ttcagggatg aattaaaaac cagtaaaaat
      901 acccgacaag aaatctgcaa tgatttactt cataaaaaac atgtcagtga aaaagaaacc
      961 tggcgttggg ttgaaggata taataaagca acagaactgg ctcgccaatg ccctgatacc
     1021 catgtattgt caatttctga tcgtgaaggc gatttttatg acctgtttga acgcgcagag
     1081 cagactcccg gaataaaagc tgactggctt gtcagaatga aatttaataa cagagcaacg
     1141 ctgaatatta atggtaaacg ggatcatagg ttgttgcacg aaagaatcat ggagattaca
     1201 ccgcagcaac tggttgaatt cactatacct gatgggcggg ggcagcaagc cagaacactg
     1261 cctgacatgc tttcatcaga cgcaggtcct ctctcatatc ctctgattta ctccagtaag
     1321 gtgatgatca tatctttatt tgatagtacg gtaatgccag ataaatacag acacaatggt
     1381 aaataatata gttgcaaata atgctgccat aatcagcgcg attgcgacac ctgttttcga
     1441 taccagcggc accagcaaag cgcacagtcc atagccaagt gtgtgactgg tagcgagcca
     1501 gcccgcgcct gcgccatcag ccagcttatc attcagtaga agttgataag cgggtgttgc
     1561 cagagctgct ccaaatgaca gcactgcaca gcccgggtaa aacaaccata gctgttcgga
     1621 gagcatgata gccagaccac cactcatcaa cactccggca ctcaggagca gggccaccgg
     1681 agtcaggcgc tgcggacgga gtaccccgaa ctgcgcgata agcgcagcta ctgcggacag
     1741 acccaacagc caggccacct gttggctaat ggcggtggta tccgtgacga actggcgagt
     1801 aagggcaggc gaaagtccaa gctgcatcat gctcaccgcc gcagccagta atattgcgca
     1861 aagcagataa ggcagacaat cccgcttcag actgacgctc ttgcactccg gtgtgggctg
     1921 tggtggtgtg ccgggcaacc gcagcagcat cagcagcgcc agcactggtg ctgccatcag
     1981 tagccccagt ggcgccagag gatgaatggc cagcattgct gccgcgcaca gcggcccgaa
     2041 tagcctgccg caactcaggc cggagctgat ggttgccaga gcggccatgc gattcccttc
     2101 tccagctctc tgcaatgccc agacctgaca ggctggcacc attgccgaca cggtcagccc
     2161 gtaggcgatc cgggcgatga ttaatattcc cagtcccacc gctgttgtta tggcgctggt
     2221 agccatcagc acgcttccca gccctagcag ggtaaagctt atgccatatc ctcccagagc
     2281 ccacaacacc acaggtttac tgccagtacg ggaaatttgc tttccccacc acggggacga
     2341 tggcagaaac agcattgagc caagcatcag cagggcagcc catacagaca gactcagatt
     2401 tgtctgtatg accagaacag ggagcacaac cagcagaccg ttctgtccga taccgagaag
     2461 cccggcactg aacgcaagtg gccagcagga cagtgttttt tggggcgtat cttcgatccc
     2521 tggtgacatt ataatgtttc aactccatgt attaattgtg tttatttgta aaattaattt
     2581 atctgacaat aacatttatt gttgataatg agaatcatta tcgacataat tgttattatt
     2641 ttactgtgtg ggagctgttt gactatgacc ctgccctctg aaaaaccagc cacagatgtg
     2701 gctgcgcagt gcttcctgaa tgcactgatt cgtgaaacca cagactggaa actgacagaa
     2761 tacccgccag acgaattgct catcccgctg gatgagcaga aatcgctcca tttcagagtg
     2821 gcttatttct ccccaaccca gcatcaccgc tttgcatttc ctgcacgtct ggtcacggca
     2881 tcaggcagtt atcctgtcga ctttaccact ctctcccggc ttattattga taagctacgc
     2941 catcaactgt ttctgcccgt tcccctctgc gaaactttcc accagcgcgt gctggaaagc
     3001 catgtccata cgcaacaggc aattgatgcc cgtcatgact gggccgctct gcgtgaaaaa
     3061 gcgttgaatt ttggcgaggc tgagcaggcg ctgctgacag gacacgcttt ccaccctgcg
     3121 cctaagtctc atgaaccgtt taaccgacgg gaggctgaac gctacctgcc tgatatggca
     3181 ccccacttcc cactgcggtg gttttcggtg gataaaacgc aaatcgccgg tgaaagtttg
     3241 catctcaacc ttcaacagcg gctgacgcga tttgccgcag agaatgcgcc tcagttactc
     3301 aacgaattaa gtgacaacca atggctgttc ccgttgcacc cgtggcaggg ggaatatctt
     3361 ttgcagcagg ggtggtgcca ggcacttgtt gctaaagggc tgattaaaga cttaggtgag
     3421 gccggcacgt cgtggctgcc gaccacctct tcccgttccc tctactgtgc caccagccgc
     3481 gatatgatca agttctccct gagcgtacgg ctgaccaact ccatccgtac tctgtccgtg
     3541 aaagaagtga agcgtgggat gcgcctggca cgcctggctc aaaccgacgg ctggcagatg
     3601 ctacaggtcc gcttcccgac tttccgggta atgcaggagg atggctgggc cgggctgctc
     3661 gatcttaacg gcaacatcat gcaggaaagt ctgtttgccc tgcgtgaaaa tctgctggtg
     3721 gatcagccga aaagccagac taacgtactg gtctccctga ctcaggccgc acctgatggc
     3781 ggtgattcgc tgctggtttc ggcggtaaaa cgcctgagcg atcgcctcgg tatcactgtg
     3841 caacaggccg cccatgcatg ggtcgatgca tactgtcagc aagtgctaaa gccgctgttt
     3901 acggctgaag cggattacgg cctggtgctg ctggcgcatc agcaaaatat tcttgtccag
     3961 atgcttgggg atctgccggt cggatttatt taccgtgact gtcagggcag cgcttttatg
     4021 cctcacgcga cagactggct cgattctatt ggcgaggcgc aggcggaaaa tattttcacc
     4081 catgagcagt tgctgcgcta tttcccttat tacctgctgg ttaactccac ttttgctgtg
     4141 accgctgcgc tgggggctgc cgggctggac agcgaatcga atctgatggc tcgtgtacga
     4201 gcatcgctgg ctgaagtgcg tgatcaggtg actcataaaa cctgtctcaa ctacgtgctg
     4261 gaaagtccgt actggaacgt aaaaggtaac tttttctgtt atctgaacga tcataacgag
     4321 aacaccattg ttgacccttc ggtgatctat ttcgatttcg ctaacccgct gcaggctcag
     4381 gaggtctgaa tgtctgaggc aaacattatt cacagcagat atggactgcg ctgtgaaaaa
     4441 ctcgacaagc ccctgaatct tggctgggga ctggacaata gcgcggtgtt gcactgtccc
     4501 ggggagctgc cgacagggtg gctgtgcgat gcgctggatc agatatttat cgccgcacca
     4561 caactgtcag cagtggctct cccctgggcc gaatggcgtg aggagccaca ggcgctgacg
     4621 ctttttgggc aggtaaaaag cgacattatc catcgtactg ccttctggca gttaccgtta
     4681 tggttgagtt caccggcaaa ccgggcctcc ggtgaaatgg tttttgatgc agagcgtgag
     4741 atttatttcc cgcagcgccc accccgtcca cagggtgagg tttaccgccg ttacgatcca
     4801 cgtattcgca ggatgctgag tttccgcatt gccgatcccg tttctgatgc agaacgtttc
     4861 acccgctgga tgaatgatcc gcgcgttgag tatttctggg agcaaagtgg ctcactggag
     4921 gtacagaccg cctatctgga gcgccagctc accggtaaac atgcattccc gctgattggg
     4981 tgcttcgacg atcggccatt cagctatttc gaaatctact gggcggcgga agaccgcatt
     5041 ggtcgtcact attcgtggca gccctttgac cgtggcctgc atctgctggt tggtgaacag
     5101 caatggcgcg gggcccacta tgtgcaaagc tggctgcgcg ggctgacaca ttacctgctg
     5161 ctggatgagc cccgtacgca gcgcacagta ctggagccac gcaccgataa ccagcgcctg
     5221 ttccgccatc ttgagcctgc gggataccgg acaattaaag agttcgactt cccacataag
     5281 cgctcgcgca tggtgatggc ggatcgtcat cacttcttca cggaggtcgg tctgtaatga
     5341 atcacaagga ctgggatttt gttaaccgtc ggctggtggc aaaaatgttg tctgagatgg
     5401 agtatgagca ggttttccac gcggaatctc agggcgatga ccactactgc attaacctgc
     5461 cgggagcaca atggcgcttc atcgctgaac gtggtatctg gggctggctg tggattgatg
     5521 cccaaacgtt gcgctgcacg gacgaaccag tactggcgca gacgctgctg atgcagctaa
     5581 agccggtgct gtcaatgagt gatgccactg ttgcagagca tatgcaggat ttgtatgcca
     5641 cgctactggg cgacctgcaa ctgctgaagg cccgtcgcgg actgagtgcc agtgacctga
     5701 ttaatcttga tgccgatcgt ctgcaatgcc tgctgagcgg tcatcctaaa ttcgttttta
     5761 ataaaggtcg ccgtggctgg ggcaaagagg cgctggaacg atatgcgcca gagtatacca
     5821 acaccttcag actgcactgg ctggcggtaa aacgtgaaca tatgatctgg cgctgcgata
     5881 acgatctgga tattcagcag ttgttgactg ccgcaatgga tccgcaggag tttacacgct
     5941 tcagtcaggt ctggcaggaa aacgggctgg atcacaactg gctaccgctg ccggtacatc
     6001 cgtggcagtg gcagcaaaaa atcgccacag acttcatcgc tgattttgtc gaaggcagga
     6061 tggtgtctct cggcgagttt ggcgaccagt ggctcgccca gcagtcgctg cgtaccctga
     6121 ccaacgccag ccgccgggga gggctggata tcaagctgcc gctgaccatc tacaacacct
     6181 catgctaccg gggaatccct ggcagatata tcgctgccgg accactggct tcacgctggc
     6241 tgcaacaggt ttttgcgacc gacgccaccc tggtgcaaag cggcgcagtg atccttggtg
     6301 aaccggctgc aggctatgtg tcccatgaag gctatgccgc gcttgcccgg gctccctatc
     6361 gctaccagga aatgcttggt gttatctggc gggagaatcc gtgccgctgg ctgaaaccgg
     6421 atgaaagtcc tgttctgatg gcaacactga tggagtgcga cgaaaacaat cagccgctgg
     6481 caggcgcata tatcgaccgc tccggactgg acgctgaaac ctggcttacg caattgttcc
     6541 gggtggttgt ggttccgctg tatcacctgc tttgccgcta cggtgtcgcg cttattgcac
     6601 acggacaaaa tataacgctc gccatgaaag agggggtacc acagcgtgtt ctgctgaaag
     6661 acttccaggg cgatatgcgg ctggtgaaag aagcgtttcc cgaaatggac tctttgcctc
     6721 aggaggtgcg tgatgccaca tcccgtctga gtgcggacta cttaatccat gatttgcaga
     6781 cgggtcactt cgtgacagta ctgcgtttta tttcgccact gatggttcgt cttggcgtac
     6841 ctgaaaggcg attttatcaa ctgctggcag cagtgttgag tgattacatg aacaaacatc
     6901 cacaaatggc agagcgtttt gcgcttttct cactcttcag gccacaaatc attcgcgtgg
     6961 tgctgaaccc ggtaaaactg acctggccgg acctggatgg cggcagtcgc atgctgccga
     7021 attaccttga gaatctgcaa aatccgctgt ggctggtaac tcaggaatat gaatcatgaa
     7081 aaaaagtgtc gattttattg gtgtcgggac agggccattt aatctcagca ttgctgcatt
     7141 gtcacatcag atcgaagaac tgaactgtct cttctttgac gaacatcctc atttttcctg
     7201 gcatccgggt atgctggtac cggattgtca tatgcagacc gtctttctga aagatctggt
     7261 cagtgcagtt gcacctacaa atccctacag ctttgttaac tatctggtga agcacaaaaa
     7321 gttctatcgc ttccttacaa gcaggctacg tacagtatcc cgtgaagagt tttctgacta
     7381 tctccgctgg gctgctgaag atatgaataa cctgtatttc agtcataccg ttgaaaatat
     7441 tgatttcgac aaaaaaagtc gattgtttct ggttcagacc agtcggggag aatattttgc
     7501 ccgcaatatc tgccttggta caggaaaaca accttattta ccaccctgtg tgaagcatgt
     7561 gacacaatcc tgtttccatg ccagtgaaat gaatcttcgt cggccggacc tgagtggaaa
     7621 acggataacc gtggttggtg gaggacagag tggtgcagac ctgttcctta atgcattacg
     7681 cggggaatgg ggagaagcgg cggaaataaa ctgggtctca cggcgtaata attttaacgc
     7741 actggatgag gctgcttttg ctgatgagta ttttacacct gaatatattt caggcttctc
     7801 cggactgaag gaagatattc gccatcagtt actggatgag cagaaaatga catcggatgg
     7861 catcactgcc gattctttac tgaccattta tcgtgagttg taccaccgtt ttgaagttct
     7921 gagaaaacca agaaatatcc gtctgctacc cagtcgctcg gtaacaaccc tggaaagtag
     7981 tggtccgggc tggaggttat tgatggaaca tcatctggat cagggcagag agagcctgga
     8041 aagtgatgtg gtgattttcg ccacaggtta ccgttctgca ttgccacaaa tacttccctc
     8101 actgatgccc ttgatcacca tgcacgataa gaacaccttt aaagtgcgtg atgacttcac
     8161 tctggaatgg agtggcccga aagaaaataa catctttgcg gtcaacgcca gcatgcaaac
     8221 tcatggcatc gccgaacccc agctcagcct gatggcctgg cgatctgcac gtattcttaa
     8281 tcgcgtactg gggcgtgatt tattcgatct cagtatgccg cccgcactga ttcagtggcg
     8341 cagcggcagc cggaaaaaac cgcagccgga ggctgcttct ttaactcact atacagcaaa
     8401 tattcaggaa taacgatgat gcgcaaaaag tatatgcccc gggctcttgg tccgctgctt
     8461 cttgtcgtgc tgtcaccagc tgtcgcccag caaaacgatg ataatgagat catagtgtct
     8521 gccagccgca gcaatcgaac tgtagcggag atggcgcaaa ccacctgggt tatcgaaaat
     8581 gccgaactgg agcagcagat tcagggcggt aaagagctga aagacgcact ggctcagtta
     8641 atccccggcc ttgatgtcag cagccagagt cgaaccaact acggtatgaa catgcgtggc
     8701 cgcccgctgg ttgtcctgat tgacggtgtg cgcctcaact cttcacgttc cgacagccga
     8761 caactggact ctgtcgatcc ttttaatatc gaccatattg aagtgatctc cggcgcgacg
     8821 gccctgtacg gtggcgggag taccggaggg ttgatcaaca tcgtgaccaa aaaaggtcag
     8881 ccggaaacca tgatggagtt tgaggctggc acaaaaagtg gctttaacag cagtaaagat
     8941 cacgatgagc gcattgccgg tgctgtctcc ggcggaaatg accatatctc cggacgtctt
     9001 tccgtggcat atcagaaatt tggcggctgg tttgacggta acggcgatgc caccctgctt
     9061 gataacaccc agaccggcct gcagcactcc aatcggctgg acatcatggg aaccggtacg
     9121 ctgaacatcg atgaatcccg gcagcttcaa ctgataacgc agtactataa aagtcaggga
     9181 gacgacaatt acgggcttaa tctcgggaaa ggcttttccg ccatcagcgg gagcagcaca
     9241 ccatacgtca gtaaggggct gaattctgac cgcattcccg gcactgagcg gcatttgatc
     9301 agcctgcagt actctgacag tgatttcctg ggacaggaac tggtcggtca ggtttactac
     9361 cgcgatgagt cgttgcggta ctacccgttc ccgacggtaa atgcgaataa acaggcgaca
     9421 gctttctcct cgtcacagca ggataccgac cagtacggca tgaaactgac tctgaacagc
     9481 caactgatgg acggctggca aatcacctgg gggctggatg ctgagcatga gcgctttacc
     9541 tccaaccaga tgttcttcga tctggctcag gcaagtgctt ccggagggct gaacaaccat
     9601 aagatttaca ccaccgggcg ctatccgtca tatgacatca ccaatctggc ggccttcctg
     9661 caatccagct atgacattaa tgatattttt accgttagcg gtggcgtacg ctatcagtat
     9721 actgagaaca gggtagatga tttcatcgac tacacgcagc aacagaagat tgctgccggg
     9781 aaggcgatat ctgccgacgc cattcctggt ggttcggtag attacgataa ctttctgttc
     9841 aatgctggtc tgctgatgca catcaccgaa cgtcagcagg catggttcaa tttttcccag
     9901 ggggtggcat tgccggatcc ggggaaatat tatggtcgcg gcatctatgg tgcagcagtg
     9961 aacggccatc tttccctgac aaagagcgtg aacgtcagcg acagtaagct ggaaggcgtg
    10021 aaagtcgatt cttatgaact gggctggcgc tttaccggtg acaacctgcg gactcaaatc
    10081 gcggcatatt actcgctttc caataagagc gtggaaagga ataaagatct gaccatcagt
    10141 gtgaaggacg acaggcgccg tatttacggc gtggaaggtg cggtggacta cctgatcccg
    10201 gatactgact ggagtaccgg tgtgaacttc aatgtgctga aaaccgagtc gaaagtgaac
    10261 ggtcaatggc aaaaatatga cgtgaaggaa tcaagtccat cgaaagcgac agcttacatt
    10321 aactgggcgc cggaaccgtg gagtctgcgt gtacagagca ccacttcttt cgacgtaagc
    10381 gatgcagagg gtaacgatat taatggttac actaccgtcg attttatcag tagttggcag
    10441 cttccggtgg gaacactcag cttcagcgtt gagaacctct tcgaccgtga ctataccact
    10501 gtctggggac agcgtgcacc tctgtactac tgcccgggtt acggccctgc ttcactgtac
    10561 gactacaaag gccggggccg aacctttggt ctgaactact cagtgctgtt ctgacgggga
    10621 tgcttttgca acaagggtat gctgtaaaca acatggtcct tggtggccat gttgtttaca
    10681 gccagtataa tatacagcct taacaagata ttccgtctcc ggaatggact gttcaccact
    10741 gaataacatt atttctgttt tatggatctt tgactgaaat ttatcgcata tcctcatttc
    10801 attgtccgtc tgaaaactaa ccttacaagt aagcgtcgtc tcagcaccgt ctggcagatc
    10861 ctgaaattcc tgagaggatg atctgcgccc aatatcctga gccggaactt tgctgcaatg
    10921 aaggccaacg aaaaatgggg gagtatgtta tagagtactt gctacagggt aaaaagaggt
    10981 acctgtcgct ggttctccgt tcagtttaac cgggagattt tctcctgcgc ctgtcataaa
    11041 gtccggtaat ggagattgtt aatacgatgg tgcacgatgc attgtcggtg cccggcccgg
    11101 aggatgcccc gtaactacat acggatcagg gttggcaata tcgaatggca ggctataggc
    11161 aaagttaaag ccccatggag tagcacaaaa tatgccacac aaaggaaacg gtctgaataa
    11221 cgcagtgatg gagaacttct tcggcacact gcaaaatcgc atcgtggtcg atcgctaatt
    11281 cgccgtgaat cactgaacga tgttccatgc gaacagatat tcgatcatat cgaaatatta
    11341 aacattaccc ctccccgcat tgggcaaaaa aaaaacaaaa aacgatcaat tagtattgat
    11401 tcacataatc tctcatcatt aatgagaaca ttgtcacata tttacaatat agaactgtta
    11461 cttttagtaa gctgttagta accaactgtt actaataatt ttaattctta atactttgtt
    11521 cattcaataa atgagttgag ag
//
LOCUS       DAESHI010000130        11471 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.132, whole genome shotgun sequence.
ACCESSION   DAESHI010000130 DAESHI010000000
VERSION     DAESHI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11471
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.132"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            1..613
                     /locus_tag="KW011_003579"
                     /pseudo
     CDS             1..613
                     /locus_tag="KW011_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            610..1224
                     /locus_tag="KW011_003580"
     CDS             610..1224
                     /locus_tag="KW011_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HBH7661828.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1281..2738)
                     /gene="pepD"
                     /locus_tag="KW011_003581"
     CDS             complement(1281..2738)
                     /gene="pepD"
                     /locus_tag="KW011_003581"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBH7661829.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2999..3457
                     /gene="gpt"
                     /locus_tag="KW011_003582"
     CDS             2999..3457
                     /gene="gpt"
                     /locus_tag="KW011_003582"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBH7661830.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3549..4793
                     /gene="frsA"
                     /locus_tag="KW011_003583"
     CDS             3549..4793
                     /gene="frsA"
                     /locus_tag="KW011_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBH7661831.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4851..5252
                     /gene="crl"
                     /locus_tag="KW011_003584"
     CDS             4851..5252
                     /gene="crl"
                     /locus_tag="KW011_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBH7661832.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5291..6346)
                     /gene="phoE"
                     /locus_tag="KW011_003585"
     CDS             complement(5291..6346)
                     /gene="phoE"
                     /locus_tag="KW011_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBH7661833.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6634..7737
                     /gene="proB"
                     /locus_tag="KW011_003586"
     CDS             6634..7737
                     /gene="proB"
                     /locus_tag="KW011_003586"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBH7661834.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7749..9002
                     /gene="proA"
                     /locus_tag="KW011_003587"
     CDS             7749..9002
                     /gene="proA"
                     /locus_tag="KW011_003587"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBH7661835.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9117..9192
                     /locus_tag="KW011_003588"
     tRNA            9117..9192
                     /locus_tag="KW011_003588"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9150..9152,aa:Thr,seq:cgt)
     gene            9798..9956
                     /locus_tag="KW011_003589"
     CDS             9798..9956
                     /locus_tag="KW011_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661836.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         2972 a         2669 c         2893 g         2937 t
ORIGIN      
        1 gtctatttta cagcggcata ttgcctcgtt ttcgcatcat ggtttgcctg aaggcagtga
       61 cgacgcgcta cggtatattg cggaacatga cgatgcgctg gcgtttgcgc gtattaatcg
      121 ccagctgatc tctttacgca taatgcaaca ggttaaggcc accggaagtc cggttctgga
      181 tgtggcgcat aactttgtta gcgcgtgtca aatcggcgat caacagggct ggttgcatcg
      241 taaaggtgcc acgccggatg acaacggtct ggtgattatt cccggttcac tcggtgatta
      301 ctcctagctt gtacagcctg ttgcgaacga aaaaacgctc cactcgctgg cgcatggggc
      361 tgggcgtaaa tgggggcgca ccgagtgtaa agggcgtctg gcagcgaaat acacagcgac
      421 gcagctctca cgtactgaac ttggcagtcg ggtaatttgt cgcgataaac aactcatctt
      481 tgaagaagcg ccacaagcct ataaatcggc tgaaagcgtg gtgcaatgtc tggtgcaggc
      541 tggtttaatt attcctgtcg cgcggctgcg tccggtgcta acgctcaaaa acagtggagg
      601 gaaaaaagga tgatcttgct acaactctcc tcagcgcagg ggccggaaga atgttgtctc
      661 gcagtgaaaa aggcgctgga gcgattaatt aaagaagctg cccgacaaga cgtcgcggta
      721 acggtgttgg aaacagaaac gggccgctac tctgacacgc tgcgttcagc tctggtttct
      781 ctggatggcg ataacgcatg ggcattaagc gaaagctggt gcggcactat tcagtggatt
      841 tgtccgagtc cgtatcggcc tcatcatggg cgcaaaaact ggtttctggg cattgggcgt
      901 tttaccgctg atgagcagga acaatcggat gcaatccgtt atgagacgtt gcgttcgtcg
      961 gggccgggcg gtcaacatgt caataaaacc gactcggcgg tacgcgccac gcatttggca
     1021 tccggtatta gcgtgaaggt tcagtcagag cgtagtcagc atgctaacaa gcggctggca
     1081 cgattgctga ttgcctggaa gctggagcag cagcaacagg aaaatagcgc ggtgctgaaa
     1141 tcgcagcggc gaatgttcca tcaccagatt gaacgtggca acccgagacg gacatttaca
     1201 gggatggctt ttatcgaagg ataatgaagg atgaaactgc cggaaggcga ttaaacgcca
     1261 tccggcagca aatcaaataa ttacttcgcc ggaatttctt tcagcagttc agtcagcagt
     1321 gtccagtaat gacctacgct ttcgatgtga acttgctcat ccggagagtg tggaccggtg
     1381 atagttggcc cgatagaaac catgtccatt tccggatacg gttttttgaa cagaccacat
     1441 tccaggcccg cgtggataat ctggatgttc ggcgttttgt tgaacagacg ctgataggtt
     1501 tcacgtacca gatgcatcac cggagaatta gcgtccggct gccagccagg atatgcgcct
     1561 ttcgcttcgg ttttcgcgcc agccagttta cccagcgaat ccagcatgct caccacgtag
     1621 tctttaccgc tgtcgatcag agaacggatc agacagtgaa tttctacgtt attatcagtc
     1681 atggtcacca caccgacgtt cagggaggtt tcgaccacac cttttgccac gtctgagttg
     1741 cggatcacac cgttcggggt ggcgttcagc agacgaataa aggtatcgcg agatttcgca
     1801 atcagggcag ctttatcgtt cgctacagag tccagcaaca aggccagatt tttctctttc
     1861 tctgccagct cgtttttcag gatctcctga taggtattca ccagagattt caggacgtcg
     1921 actttatcag ctgcgacagc aatggtcgca aaggcttcac gcgggatggc gttacgcagt
     1981 gtgccgccgt tgaaatcgat aaggcgcaga tccagttctt ctgcatgacc cgctaggaag
     2041 cgcaccagca gtttgttggc attacccagg ccaacgtgga tttccccgcc ggagtgaccc
     2101 cctttcagac cttttaaggt taacttgaag gtttcaaaac cagctggaac cgcttcacga
     2161 tctaaatgca ggttggaggt gaagtcgata ccccccgcac aacccatgta gatttcacct
     2221 tcttcttcgg agtcggtgtt aatcagaata tcagcctgca accagttgct ctgtaagccg
     2281 aacgcaccgt ccataccggc ttcttcggtc atggtcagca gcacttccag cgggccgtga
     2341 accacgtttt cgtcagccag aaccgccagc gcagacgcca taccaatgcc gttatctgca
     2401 cccagcgtgg tgccgcgcgc tttaacccat tcgccatcaa tataaggctg gataggatct
     2461 ttcgtgaagt catgcacggt gtcgttattt ttctgcggca ccatatcgag gtgggcctgt
     2521 aagacgaccg atttacgatt ttccatacct gcggtagcag gtttacgaat caggatatta
     2581 cctacctgat cgcgttcgac atggaaacct ttctcttttg cccaaccaac aatgtattca
     2641 gcgagttgct cttcatgata ggacgggtga ggaatagaac agattttggc aaaaatatcc
     2701 cacagcggct gtggagataa ttgagacagt tcagacacgt taagtctcct tgtcgatcac
     2761 ccgcaaaaca gtattgcagg tcacagggtt agcagaaaat gttgtcaaca caagacaggc
     2821 ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa
     2881 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca
     2941 acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat
     3001 gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag
     3061 ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctggt
     3121 accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc
     3181 cagctacgat cacgacaacc agcgcgagct taaagtgcta aaacgcgcag aaggcgatgg
     3241 cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg
     3301 tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct
     3361 ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat
     3421 gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc
     3481 gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg
     3541 ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc
     3601 ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg
     3661 cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct
     3721 ggcgcggcat tgacccacgt gaaatcctcg acgtccaggc gcgtattgtg atgagcgatg
     3781 ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggt ggcaactgga
     3841 tttatgagtg ggccatccag gcgatggtgt ggcaacaaaa agcctgtgcg gaagaagatc
     3901 cgcaactcag tggtcgtcac tggctgcatg cggctacgtt gtacaacatt gctgcctatc
     3961 ctcatctgaa aggagatgac ctggccgagc aagcgcaggc tttgtcaaac cgtgcctatg
     4021 aagaagccgc ccagcgtctg ccgggcacga tgcggcagat ggagtttacc gtacccggcg
     4081 gtgcgcccat caccggcttt ttgcatatgc cgaaaggcga tggcccgttc ccgacagtat
     4141 taatgtgtgg tggtctggat gcgatgcaga cggactatta cagcctgtat gaacgttatt
     4201 ttgcgccgcg cggcattgcg atgctgacta ttgatatgcc gtcggtgggc ttttcatcaa
     4261 aatggaagct cacccaggac tccagcctgt tgcatcagca cgttttaaag gctctgccta
     4321 acgtaccgtg ggtggatcac actcgcgtcg cggcctttgg tttccgtttc ggcgctaacg
     4381 ttgccgtgcg tctggcatat cttgaatcgc cgcgtctgaa agcggttgcc tgtcttggtc
     4441 cggtagttca taccctgttg agtgatttta agtgccagca acaggtgccg gaaatgtatc
     4501 ttgacgttct ggcgagtcgt ttggggatgc atgatgcttc cgacgaagcg ttacgcgtgg
     4561 agctgaatcg ctattcatta aaagtgcaag gattgctggg gcgtcgctgc ccaacgccaa
     4621 tgttatcagg ctactggaag aacgatccgt tcagcccgga agaggactca cgcttaatca
     4681 cctcatcatc tgctgacggt aaattattag agatcccatt taacccggtg tatcggaatt
     4741 ttgacaaagg tcttcaggaa atcaccggct ggatcgaaaa acgcttgtgt taaaaatttg
     4801 ctaaaatttg ccaatttggt aaaacagttg catcacaaca ggagattgca atgacgttac
     4861 cgagtggaca cccgaagagc agattgatca aaaaatttac cgcactaggc ccgtatattc
     4921 gtgaaggtaa gtgcgaagat aatcgattct ttttcgattg tctggctgta tgcgtcaacg
     4981 tgaaaccggc accggaagtg cgtgagttct ggggctggtg gatggagctt gaagcgcagg
     5041 aatcccgttt tacctacagt taccagtttg gtctgttcga taaagcaggc gactggaaga
     5101 gtgttccggt aaaagacact gaagtggttg aacgactgga gcacacccta cgtgagtttc
     5161 acgagaagct gcgtgaactg ctgacgacgc tgaatctgaa gctggaaccg gcggatgatt
     5221 ttcgtgatga accggtgaag ttaacggcgt gagtgaaatg tgccggatgt atcacatccg
     5281 gcaatatcca ttaaaactga taggtcatgc caaccgcgac aatatcatca ttattaatat
     5341 tcaatttgtt atcgctatcc agttggttga ttttataatc aacaaacgct gacatatttt
     5401 tgttgaaata atacgtagca ccgacgtcga tataattgac cagatcttca tcaccgatac
     5461 cttcaatatc tttccctttc gataagacat aacccagcga tggacgcaga ccaaagtcaa
     5521 actggtattg agcgaccgct tcaaagttct gtgtcttatt ggcaaagccg ccagttattg
     5581 gcgtcatttt gcgtgtttca gaatagaaag ttgccagata aatattattg gcatcgtatt
     5641 tcagacctgt tgcccatgct tctgcacgct tgcctgtgcc acggctttgc aggttctgct
     5701 cgttggtgcg atctgagttg gtataggccc cactaatggc gaaatcgctg ccgccaaagt
     5761 catatgtcaa tgacgtgccg aagccatcgc cgttttgctt tttaacgtcg cggttttcgt
     5821 ttttcccttg atattgcagg gttaagttca ggccatcgat aacgccgaag aagtcggtgt
     5881 tccgatacgt cgccagaccg ctggcgcgtt tggtcataaa gttgtcggtc tgcgccgagg
     5941 aatcgccacc aaattccggg aacatatcgg tccaggcttc cacgtcatac aacgcgccca
     6001 ggttacgacc ataatcgaaa gaacccaaat ctttatattt caacccggca aaagcgagac
     6061 gcgttttctg ctgtgcagta tcactctccg ctttatttcc ggcaaactcc gcttcccacc
     6121 gaccataacc agtcagttga tcgttaattt gtgtttcacc tttaaaacca aaacggatat
     6181 aactctggtc gccatcttta ctggcgttat cactcatata atgcatggct ttaactttgc
     6241 catagacatc cagtttatta ccgtctttat tatatatttc tgcagcctgt acagatgcag
     6301 atgccacaat gcccatcacc actaatgcca gagtgctctt tttcattttc attcctgatt
     6361 ttaattaacg cgcgaatatt cagcgggaga gccccgttga aaacaggaaa gtttttaacc
     6421 tgagattgtt aaagatatat tacagattaa taatattctt aaaatgtggt aatttattaa
     6481 atctgtaata aaagcgtaaa caactgccgc taggcttgct gatcccgcgc aacaaaacgc
     6541 catgctttgc tcacagatgg ttggcaaccg acgacagtcc tgctaaaacg ttcgtttgat
     6601 atcatttttc ctaaaattga atggcagaga atcatgagtg acagccagac gctggtggta
     6661 aaactcggca ccagtgtgct aacaggcgga tcgcgccgtc tgaaccgtgc ccatattgtt
     6721 gaacttgttc gccagtgcgc gcagttacat gccgccgggc atcggattgt tattgtgacg
     6781 tcgggcgcga tcgccgccgg acgtgagcac ctgggttacc cggaactgcc agcgactatc
     6841 gcctcgaaac aactgctggc agcggtaggg cagagtcgac tgattcaact gtgggaacag
     6901 ctgttttcga tttatggcat tcacgtcggg caaatgctgc tgacccgtgc tgatatggaa
     6961 gaccgtgaac gcttcctgaa cgcccgcgac accctgcgtg cgttgctcga taacaatatc
     7021 gttccggtaa tcaatgagaa cgatgctgtc gctacggcag agattaaagt cggcgataac
     7081 gataaccttt ctgcgctggc ggcgattctg gcgggtgccg ataaactgtt gctgctgacc
     7141 gatcaaaaag gtttgtatac cgctgacccg cgcagcaatc cgcaggcaga actgattaaa
     7201 gatgtttacg gcattgatga cgcactgcgc gcgattgccg gtgacagcgt ttcaggcctc
     7261 ggaactggcg gcatgagtac caaattgcag gccgctgacg tggcttgccg tgcgggtatc
     7321 gacaccatta ttgccgcggg cagcaagccg ggcgttattg gtgatgtgat ggaaggcatt
     7381 tccgtcggta cgctgttcca tgcccaggcg actccgcttg aaaaccgtaa acgctggatt
     7441 ttcggtgcgc cgccggcggg tgaaatcacg gtagatgaag gggcaactgc cgccattctg
     7501 gaacgcggca gctccctgtt gccgaaaggc attaaaagcg tgactggcaa tttctcgcgt
     7561 ggtgaagtca tccgcatttg caacctcgaa ggccgcgata tcgcccacgg cgtcagtcgt
     7621 tacaacagcg atgcattacg ccgtattgcc ggacaccact cgcaagaaat tgatgcaata
     7681 ctgggatatg aatacggccc ggttgccgtt caccgtgatg acatgattac ccgttaagga
     7741 gctggccgat gctggaacaa atgggcattg ctgcgaagca agcctcgtat aaattagcgc
     7801 aactctccag ccgcgaaaaa aatcgcgtgc tggaaaaaat cgccgatgaa ctggaagcac
     7861 aaagcgaaat catcctcaac gctaacgccc aggatgttgc tgacgcgcga gccaatggcc
     7921 ttagcgaagc gatgcttgac cgtctggcac tgacgcccgc acggctgaaa ggcattgccg
     7981 acgatgtacg tcaggtgtgc aacctcgccg atccggtggg gcaggtaatc gatggcggcg
     8041 tactggacag cggcctgcgt cttgagcgtc gtcgcgtacc gctgggggtt attggcgtga
     8101 tttatgaagc gcgcccgaac gtgacggttg atgtcgcttc gctgtgcctg aaaaccggta
     8161 atgcggtgat cctgcgcggt ggcaaagaaa cgtgtcgcac taacgctgca acggtggcgg
     8221 tgattcagga cgccctgaaa tcctgcggct taccggcggg tgccgtgcag gcgattgata
     8281 atcctgaccg tgcgctggtc agtgaaatgc tgcgtatgga taaatacatc gacatgctga
     8341 tcccgcgtgg tggcgctggt ttgcataaac tgtgccgtga acagtcgaca atcccggtga
     8401 tcacaggtgg tataggcgta tgccatattt acgttgatga aagtgtagag atcgctgaag
     8461 cattaaaagt gatcgtcaac gcgaaaactc agcgtccgag cacatgtaat acggttgaaa
     8521 cgttgctggt gaataaaaac atcgccgata gcttcctgcc cgcattaagc aaacaaatgg
     8581 cggaaagcgg cgtgacatta cacgcagatg cagctgcact ggcgcagttg caggcaggcc
     8641 ctgcgaaggt ggttgctgtt aaagccgaag agtatgacga tgagtttctg tcattagatt
     8701 tgaacgtcaa aatcgtcagc gatcttgacg atgccatcgc ccatattcgt gaacacggca
     8761 cacaacactc cgatgcgatc ctgacccgcg atatgcgcaa cgcccagcgt tttgttaacg
     8821 aagtggattc gtccgctgtt tacgttaacg cctctacgcg ttttaccgac ggcggccagt
     8881 ttggtctggg tgcggaagtg gcggtaagca cacaaaaact ccacgcgcgt ggcccaatgg
     8941 ggctggaagc actgaccact tacaagtgga tcggcattgg tgattacacc attcgtgcgt
     9001 aaataaaacc gggtgatgca aaagtagcca tttgattcac aaggccattg acgcatcgcc
     9061 cggttagttt taaccttgtc caccgtgatt cacgttcgtg aacatgtcct ttcagggccg
     9121 atatagctca gttggtagag cagcgcattc gtaatgcgaa ggtcgtaggt tcgactccta
     9181 ttatcggcac cattaagaaa attaataact tagcttaact tctctaagaa attgtatctt
     9241 ctgttatcac ttacaaacgc ctatacgttg ttatgggctt tggtagtgta acacgtatgg
     9301 gcgagtagtt cccaggctca acctcgtaac aagttccttt gaagctggaa gacgtgccac
     9361 cgtctctaaa ccttctacca aaactcttag caagatgttc cacacctggc gcggtgtcca
     9421 atgatgctac acgtcgtgag acgttgcaga ggataaacag gctaaatgat gggaagagtt
     9481 ttaagtgcaa tagtggatta aagcccacct cttcaaaacc taacttgatc gggaaagaat
     9541 caagctctag cacggtttcg tccaccggag tacaacgggt gcctgataag agaagaactg
     9601 ctaagacttc atagcttttg tgaaaaagcc ctttttagtg gatacgccta cggcgcttat
     9661 tttttggtat aaccatcaaa ggcgctttat ggctcctatt gtcttgaaaa tgtatcttta
     9721 cttgtattgt gttttatgcg tagtctccct gtgtagtatg taacttgtta attttcatat
     9781 gaatacagag gcacacgatg aaaaacattg cagcaatcaa acgtaataac cgcaagattc
     9841 acgctcgtaa gttcctgtct acgccagaag gaaaagcctg gctagagcgt aaacagagag
     9901 agaacgaaga aagaaaactc cttagtgagt tgaaatggct tagagaggat ttctgaaaat
     9961 ctccagaacg caacaggacg cattctaacg ctgttttcat tactcggtat agggataggt
    10021 gatgtgtgtt taatgcgtct gtgcgcttgt tttgtgcctt attttgcatt ttcataatgc
    10081 ggaagagaac acggaaaacc ttaaaattaa ttattggggt atttaaggct attttcaggt
    10141 gatttaaggg tattaaactt gattaaaata taaacagata attatgtatc ttattgattt
    10201 ataaggttat ttttaagtaa aggtaaataa aaaataaata gccataaata gctataaata
    10261 catcttaata cctatatccc ttcccttatg gctagattgc gagaggtgct taacgcgatt
    10321 ttcagcgccc cccgatctgg aaaatccaga atccccaaat atcagcaagg aattttcttg
    10381 cgtaatcaac atcgttttta cctattaaat cattacacag aaagcctttc tgtaatgtct
    10441 ctcactaaac tttcactaaa cacgagaatg atcggtttta tgtcggatca gatgctagaa
    10501 acagcaccac gtctcacaag ggctgtaagc gatgaaacca gtgtttatgc gggtgcaggt
    10561 caaaatatag gccaaaatcc acttaacata ataaatgtta tatgtgctaa ggaacacata
    10621 taacgccttg aattgatgta tcaggggaaa agtgatggat tttttacgta ggagcacgaa
    10681 agggcttcta ttgacttttg tatgctatga aatataatta aagttttttg tttttattgt
    10741 gtgatgtggt gggttacaca ggggaataga ttaaatggtg ttctgtttat acaggtttat
    10801 ttgttagatc aacaatttgg catttctaaa cagatgtttg aacgttgcca aatgtcttga
    10861 caacacaaga aaagggcttg ctatgccagg cattacacga aataaggcta taaaaacaaa
    10921 ataaatttta aaatttgcac aaacgagaaa ctcgttcaga aacaagcaga acaattttta
    10981 gccaacaatt tctgatggtg ctctgtaagc cgcattacac aaggctttgc gaaagtgatg
    11041 taaaagtgaa gaaagtaaga attgaacttt taagctactt ttcattggat tttttagtag
    11101 ggaattacac agggaataga gagcattatt ctatgattta cacagggaat atagggggat
    11161 tatggcacac acatagaaag acattacaca gagtgctatg tgacaaatgg atgtaacgag
    11221 aggttacatt catttatctg aaatgctctc tgtaagaaag ttggtagtaa cgatatgtta
    11281 catagactaa ttattgaatg taacgatatt acacatcggt agtaacgata cgttacatcg
    11341 gaagatgatt ggatgtaacg gtagctcaca gtgaactagg ggtaggtcat agtgaactac
    11401 ccccacagaa ttctgttacc cctatcaagt ctgatacccc tatcaaaact gatacccgat
    11461 aagtataaat a
//
LOCUS       DAESHI010000131        11428 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.133, whole genome shotgun sequence.
ACCESSION   DAESHI010000131 DAESHI010000000
VERSION     DAESHI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.133"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(42..383)
                     /gene="bamE"
                     /locus_tag="KW011_003590"
     CDS             complement(42..383)
                     /gene="bamE"
                     /locus_tag="KW011_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HBH7661837.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(532..2193)
                     /gene="recN"
                     /locus_tag="KW011_003591"
     CDS             complement(532..2193)
                     /gene="recN"
                     /locus_tag="KW011_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBH7661838.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="KW011_003592"
     CDS             complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="KW011_003592"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HBH7661839.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3280..3873
                     /gene="grpE"
                     /locus_tag="KW011_003593"
     CDS             3280..3873
                     /gene="grpE"
                     /locus_tag="KW011_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBH7661840.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3928..5214)
                     /locus_tag="KW011_003594"
     CDS             complement(3928..5214)
                     /locus_tag="KW011_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBH7661841.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5235..6026)
                     /locus_tag="KW011_003595"
     CDS             complement(5235..6026)
                     /locus_tag="KW011_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HBH7661842.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6193..7554
                     /gene="ffh"
                     /locus_tag="KW011_003596"
     CDS             6193..7554
                     /gene="ffh"
                     /locus_tag="KW011_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBH7661843.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7803..8051
                     /gene="rpsP"
                     /locus_tag="KW011_003597"
     CDS             7803..8051
                     /gene="rpsP"
                     /locus_tag="KW011_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBH7661844.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8070..8618
                     /gene="rimM"
                     /locus_tag="KW011_003598"
     CDS             8070..8618
                     /gene="rimM"
                     /locus_tag="KW011_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBH7661845.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8649..9416
                     /gene="trmD"
                     /locus_tag="KW011_003599"
     CDS             8649..9416
                     /gene="trmD"
                     /locus_tag="KW011_003599"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBH7661846.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9458..9805
                     /gene="rplS"
                     /locus_tag="KW011_003600"
     CDS             9458..9805
                     /gene="rplS"
                     /locus_tag="KW011_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBH7661847.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9882..10364)
                     /locus_tag="KW011_003601"
     CDS             complement(9882..10364)
                     /locus_tag="KW011_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBH7661848.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="KW011_003602"
     CDS             complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="KW011_003602"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HBH7661849.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
BASE COUNT         2804 a         2748 c         3060 g         2816 t
ORIGIN      
        1 attgagcacc ttttttaacg tctttgagag caactttatt attagttacc actcagcgca
       61 ggtttgttat caatattggt caacacaccg ctactgttaa aggtcagcgt cagcgtttgc
      121 tgcgttacac cttcatgacc tggttgctgg cggaagacat agaaccaggt attcgtacca
      181 aatggatcgg acatcagcgg tgtacccaat gcgtacgcaa cttgttgttg cgtcatgcca
      241 acacgtattt tggatacgtc gttagcggtc agatagttcc cctggttgat gtcaggacgg
      301 taaaccactc gctccagagt ggaacagcct gcggtcaaca tcaatagtac tgctgctgca
      361 gcagtcagcg ttttacagcg catagtgatt tgattccttt tcgggcccga gcagtacgtg
      421 gctcatctgt aatatgccga tgataataga cctttcatca ctttaaaacc ttttgctttc
      481 cggtcttacg gcgttttgct gtttactcta accgtgaagc aggaaaaaag tttacgctgc
      541 aagcagttct ttcgcattcg ctagtgtatt acgtgtgact tcactgccac caagcaggcg
      601 cgccagctct tgtaaccgcg cttttttatt cagggattgc atatgcgttt ctgtcatcgc
      661 accatcggtt tctttgctga caaaatagtg ttgatgacca catcccgcga cttgtggcag
      721 gtgggtaaca cacatcacct gagttgattc gccgagttga cgcagcagtt tgccgacaac
      781 tgccgctgtt ggaccgctaa tccctacatc cacttcatca aaaatcagtg ccggggtttc
      841 cattttacgc gccgtgatga cctggattgc cagtgcgatg cgggacaatt caccaccgga
      901 tgcgactttg gcaataggct gcattggctg acctgggttg gtggttaccc gaaactcaat
      961 acgatcggca ccgtcagcgc ccaggtgatg ctcgtcaaat ttaacatcga tcgtaaactg
     1021 cccatgcggc attgagagcg catgcatact gtcggtgatc agctgtgcaa gttcttctgc
     1081 atattgctgg cgttgttggt gtaatgcgcg cgcgatttcc agtgcctgct gatgatgttt
     1141 cgttaccgcc agcgcaagcg tttcttgtga gtcggcctga tcgtccagtt gctgctgttc
     1201 ttccagtagc gactggtaat actgtggcaa tgcctcaggg ctgacgtgat gtttacgtgc
     1261 cagcgaaatc tgttttgaga tgcgctgttc aagttcaaat agtcggttgg gatcgagatc
     1321 cagacgatcg cagtagtggc gcagttcatc gctggcttca gcaatctgga tggtagcttc
     1381 ttccagcata tcaagtacgc cggacagttt gctgtccatg ccaatcaatt cgctcaccag
     1441 ttgtttagcc gtgtaaagct gactttgcag gtttgcgtct tcaccgtcgg ccattaatgc
     1501 caatgcattc tggctggtgg tcagcaattg accgctgttc gccagacgtt tgtactcttc
     1561 gtcgatttgt tcaaactctc cgggctgcgg attaaattcg ttaagttctt ttaattggta
     1621 ttgcagcagt tccgcacggg cggcgcgttc ctgacttaac tgttgatgat gcgcgaggtc
     1681 acggcagctt tgatgccaca actgataacg tgcggtcatt tcctgcagta gagaggtttc
     1741 attggcatag ccatcaagca ggaatttttg gtgctcaggt ttggtgagta attgatgagc
     1801 gtgctgacca tggatctgaa tcagcaactg acccagttcg cgcagttgtg acagaggaac
     1861 agctgtaccg ttgatgaaac cacgggagcg accatcgctg ctgatcacgc gacgaagcaa
     1921 acattcatgc ccgtcttcaa gctggttttc ttccagccag cgcagagccg ctggcgtatc
     1981 tttcagagaa aaacgggcgc acaggtcagc gcgagcagcg ccggtacgca ccatgtcggc
     2041 ttcagcgcga ccaccgagac aaagaccgag ggcatctatt gcaatagatt tacccgcgcc
     2101 ggtctcgcca gttattacgg tcatgccgct atgaaaatca atctcaagct cacgaacgat
     2161 agcaaagttg ctgatggtca gttgtgccaa catagtcgtt ttcctgtatg aaaaaccatt
     2221 actgttattg tgtacagtat aaactggttt tatatacagt aaagaggctg gcgtaaaatt
     2281 agaataattt ttttgaccag ccgagcttgg tgcttaatgt gttgaaataa ctgtaatctt
     2341 tcggatgaat cagattcaga tggtaatcac agcgacgaat caggacatct tcaccttcct
     2401 gaatcggcag tgctatctgg ctgtcgcaac tgatttccag gtcgttacgg cgatgcgaaa
     2461 aacgcagacg gatcgtgctg ctgctgttta tgaccagtgg tcgtgctgac aacgtatgcg
     2521 ggaacatggg caccagggta atcgcatcca gagagggggt cagaatagga ccgcctgcag
     2581 agagggaata ggcggtggag cctgttggcg tcgaaataat tagtccatca gatcgctgag
     2641 aaaacgcaaa gatctcgtcg atatacactt cgaactcaat catatgcgcc actttgcctg
     2701 gatgaagcac cacttcattt atcgcggtgc tgatgcgttt ctggcaatct tgctgacaga
     2761 cttgcgcttc cagcaaaaaa cgtttctcgc tgatgtagtg gccttccagc acatcggcta
     2821 actgttgctg ggcgttatcg gggtcaaggt cagtcaggaa acccaggttg ccacggttga
     2881 ttccaataac tttaatatcg taacgggcga gtgtgcgtgc cgcgcccagc atattaccgt
     2941 cgccaccaac gactaccgcg agatcagcta gttgcccaat ctccgcgagc gtgccagttt
     3001 tcacattctt cagttgcagt tcgtgagcga tttgttgctc aacgatgacc tcgtaacctt
     3061 ttgtgcacag ccagcggtag agcatttcat gtgttgtcag tgcagtgggg tgccgtgggt
     3121 gtcccacaat gccaatacac ttgaaatgat tattcatttt tccgaggtcc ttgttgcgaa
     3181 gattgatgac aatgtgagtg cttcccttga aaccctgaaa ctgatcccca taataagcga
     3241 agttagcgag atgaatgcga aaaaaacgcg gagaaattca tgagtagtaa agaacagaaa
     3301 acgcctgagg ggcaagcccc ggaagaaatt atcatggatc agcacgaaga gattgaggca
     3361 gttgagccag aagcttctgc tgagcaggtg gatccgcgcg atgaaaaaat tgcgaatctc
     3421 gaagctcagc tggctgaagc ccagacccgt gaacgtgacg gcattttgcg tgtaaaagcc
     3481 gaaatggaaa acctgcgtcg acgtactgaa ctggatattg aaaaagccca caaattcgcg
     3541 ctggagaaat tcatcaacga attgctgccg gtgattgata gcctggatcg tgcgctggaa
     3601 gtggctgata aagctaaccc ggatatgtct gcgatggttg aaggcattga gctgacgctg
     3661 aagtcgatgc tggatgttgt gcgtaagttt ggtgttgaag tgatcgccga aactaacgtg
     3721 ccgctggacc cgaatgtgca tcaggctatc gcaatggtgg aatctgatga cgttgcgcca
     3781 ggtaacgtac tgggcattat gcagaagggt tatacgctga atggtcgtac gattcgtgcg
     3841 gcgatggtaa ctgtagcgaa agcaaaagcg taatttctgt tttcgtaata attcacggcc
     3901 ctgcatgcga atgcagggcc gttttcgtta ctccgccaca ctctcgcgca gcggtttcac
     3961 aggaaaaact tttacctgct taatcatatt gtcctgtacg tcgagaatat ctatatcgta
     4021 ctcgccaata cgcacgcggg tgcctgcgac agggatctct tccagtgcct caagaatgac
     4081 gccattaacc gtgcgggcat catcttccgg tagatgccaa ttaaaggctt tgttgatttc
     4141 ccgcacgttg gcggtgccat cgataatcac cgaaccgtcg ttttgcggcg tgacctcttc
     4201 ggcaagtgtt ggcgacatcg acgtagtgaa atcgccgaca atctcttcca gaatatcttc
     4261 aaccgtcacc agcccctgaa tgtctccata ctcgttgacg accaggccga ctttcttttt
     4321 gttgcgctga aactttacca actgcgtgct gagcggcgta ccttccggca caaaatagat
     4381 ctcgtccgcg gcgcgcagca tggtttcttt ggtgaactct tttttctccg acatcaaccg
     4441 ccaggcttca cgtactcgca gcatactgat ggcgtcgtcc agcgaatcac ggtagagcac
     4501 gatgcgcccg tgaggtgagt gggagagttg gcgcagaatc gatttccagt catcgttgat
     4561 atcaataccg ataatttcac tgcgcggcac catgatgtca tcaacggtca ttttttccag
     4621 atcgagcacc gacagcagca tatcctgatt gcgacgggaa atttgtgagc gtgattcgtg
     4681 cacgatagtg cgcaactctt ctttgctcaa agagccgcta accacgatat cggttttgat
     4741 acccatcatg cgcatcagca tacgggtgat agcattcagc aaccagacca gcggcatcat
     4801 caaaatttgc agcggagcca gcagaaaact actcggataa gcgacttttt ccgggtacag
     4861 cgcggcaatg gttttcggca atacctcagc aaataccagt acgacaaaag tcagcacacc
     4921 agtcgcaatt gccacgcccg catcgccgta caaacgcatc ccaacaatag tgccgagcgc
     4981 ggaggcaaga atattgacca ggttattgcc gattaacacc aggcttatca ggcggtctgg
     5041 cttacgcagc aatttttcga cgcgtttggc cgagcgatta ccctgtttcg ccatatgtcg
     5101 cagacgatag cggttgaggg tcatcattcc ggtttcggac ccggaaaaat aggctgaaat
     5161 gaccaccatg atgatcagaa taatgatcaa cgtagtagta gaaatgtgtt ccaggggaaa
     5221 ctccttttct gggtttagct gattaactgc tggacaattc ggctgccgaa gtaggccagt
     5281 gtcagaatta ccgcgcccgc aacgttaaac cagacgacgc ggcgtccacg ccatccttca
     5341 tggtaatgtc cccacagcag cacaatatag acaaaccacg ccacgataga gagcacagcc
     5401 ttgtcgatat tttccatgct aaataggttg tgcatgtaga acaggccagt gcaaagcgtg
     5461 agcgttagca gcaccacgcc aatctgcgtg atgtggaaca ttttacgctc gatactcatc
     5521 aatggcggca tttcctggtt aaatgccagc ttcttgttct tcagttggta atcaatccac
     5581 gccagttgca gcgcgtacag ggcggcgata attagcgtgg cataggaaaa gagcgataag
     5641 ccaatgtgca ccagcatccc aggcgttgct tccagatggg tgatgtattc attgggcatg
     5701 aaggtcgcca gcgccaggtt gataagcgca aaggcataga caatgggtag cagcaaccag
     5761 ccacgattgc gagaagccac aatggtcatt accgtacaga tcatcaaact gaccaatgaa
     5821 ccaacgttca gcaggctgag gttttgtccg ctatcaccgt cgggcaggat gcgggcttcc
     5881 agagcgattg cgtggcagac cagcgcaatg accgcagaaa taatagccat gcgccgccag
     5941 ccgccgtttt tttgcagcag accgggaaca atcagcgcaa gactgacgga gtaggcgaca
     6001 agcgcgagca gagcaaaaac gggcatagtg atgtcgacag tttgagcaat aagaaagaga
     6061 agcagtatag cgtcaggtga acgctgctcc aaccgttgca taacaacaaa gacgccttca
     6121 tgttatactg cggcaaaata ctgatgatgt gtcgcgattg cggccaaccg tttccacccc
     6181 aggcgagaga caatgtttga taatttaacc gatcgtttgt cgcgcacgct gcgcaatatc
     6241 agtggccgtg gacgcctcac tgaagacaac gtaaaagata cgctgcgcga agtgcgcatg
     6301 gcgctgctgg aggcggatgt cgctctgccg gtagtgcgtg agtttatcaa tcgcgtaaaa
     6361 gagaaagcgg ttggtcatga agttaataag agcctgacgc cggggcagga gttcgtcaaa
     6421 atcgtccgta acgaactggt tgcggcgatg ggcgaagaga accagaccct gaacctggcg
     6481 gcgcaaccgc ctgcggtcgt gctgatggcg ggcctgcaag gtgccggtaa aacaaccagc
     6541 gttggtaaac tcggtaagtt cctgcgcgag aagcacaaga agaaagtgct ggtggtttct
     6601 gccgacgttt atcgcccggc ggcaatcaaa cagcttgaga cgctggcaga gcaggtgggc
     6661 gttgatttct tcccttctga tgttggtcag aagccggtag atatcgttaa cgcggcgctg
     6721 aaagaagcca aactgaaatt ctacgacgtg ctgctggtgg ataccgctgg tcgtctgcac
     6781 gttgacgaag cgatgatgga cgagatcaaa caagtccatg cgtcgattaa cccggttgaa
     6841 accctgtttg tggttgacgc catgaccggt caggatgcgg ccaatacggc aaaagcattc
     6901 aatgaagcgt taccgcttac cggcgtagtg ttgaccaaag tggacggcga tgcccgcggc
     6961 ggtgcggcgc tctctattcg tcacatcact ggcaaaccga tcaagtttct cggtgttggc
     7021 gagaaaactg aggcgctgga gccgttccat ccggaccgca tcgcgtcgcg tattctcggc
     7081 atgggcgacg tactgtcgct gatcgaagat atcgaaagca aagttgaccg cgcgcaggca
     7141 gagaaattag ccagcaagct gaaaaaaggc gacggcttcg atctcaacga ttttcttgag
     7201 cagctgcgcc agatgaaaaa tatgggcggc atggctagcc tgatgggcaa gctgccgggc
     7261 atggggcaga tcccggataa tgttaagtcg cagatggacg ataaagtgct ggtgcgtatg
     7321 gaagccatca tcaactcgat gacgatgaaa gagcgcgcta agccagaaat tatcaaaggt
     7381 tcgcgtaagc gtcgtatcgc tgccggttgc ggtatgcagg tgcaggacgt taaccgcctt
     7441 ctgaagcagt tcgacgacat gcagcgcatg atgaagaaaa tgaagaaggg cggaatggcg
     7501 aagatgatga gaagcatgaa gggtatgatg cccccaggct tccctggtcg ctaatcggcc
     7561 tggcttgttt gccattttgg caccgggatg tgctcgtcat cctcacgtac tgcgtgtacg
     7621 ctccggtgac tgcgcgcact ccggcaccaa actggctgca ccgccgacgg ccttttacag
     7681 cagagttagc aactgttgat tgcaattccc gcaaaaatga gtaaaatttt cgggctttta
     7741 atatgacacc ggactccgtt cctcgatggg gtccggttgt tttattcaca caagaggatg
     7801 ttatggtaac tattcgttta gcacgtcacg gcgctaaaaa gcgtccgttc taccaggttg
     7861 ttgtcgctga cagccgtaat gcacgcaacg gtcgcttcat cgagcgcgtt ggtttcttca
     7921 acccaatcgc tagcgaaaaa gaagaaggca ctcgcctgga tctggatcgc atcgctcact
     7981 gggttggcca gggcgcaact atttctgatc gcgttgctgc gctgatcaaa gaagtaaaca
     8041 aagcagctta atctgtcacg gtggtcatga tgagcaaaca actcaccgcg caagcacctg
     8101 ttgatcccat cgttttggga aaaatgggtt cgtcttacgg tattcgtggg tggctcagag
     8161 tgttttcttc caccgaagac gccgaaagca tttttgacta tcagccctgg tttatccaga
     8221 aggcgggtca gtggcagcaa gtccagctgg aaagctggaa gcaccacaat caggacatga
     8281 tcatcaagct gaaaggcgtt gacgatcgtg atgcggcgaa cctgctgacg aattgtgaaa
     8341 ttgtcgtgga ttcatcgcag ctgcctcagc ttgaagaggg ggactactac tggaaagacc
     8401 tgatgggctg ccaggtagta accactgaag gctacgatct cggtaaagtc gtcgatatga
     8461 tggaaaccgg atctaatgac gttctcgtca ttaaggcaaa cctgaaagat gcgtttggta
     8521 tcaaggaacg tctcgtaccg ttcctcgatg ggcaggttat caagaaagtc gatctcacta
     8581 ctcgttcaat cgaagtagat tgggatcctg gtttttaaac caccggataa acggtaaaag
     8641 acggcgctat gtggattggc ataattagcc tgtttcctga aatgttccgc gcaattaccg
     8701 attacggggt aactggccgg gcagttaaaa atggcctgct gagcatccag agttggagtc
     8761 ctcgcgactt cacgcatgac cggcaccgta ccgtggacga tcgtccttac ggcggcggac
     8821 cggggatgtt aatgatggtg caacccttgc gggacgccat tcatgcagca aaagccgcgg
     8881 cgggtgaagg cgcaaaggtg atttatctgt caccacaggg acgcaagctt gatcaagcgg
     8941 gcgtcagcga actggcaacg aatcaaaaat tgattctggt gtgcggtcgc tacgaaggta
     9001 tagatgagcg cgtgatccaa accgaaattg acgaagaatg gtcaatcggc gattacgttc
     9061 tcagtggtgg tgagttacca gcaatgacgc tgattgactc cgtttcccgg tttattccgg
     9121 gagtactggg acatgaagcc tcggcaacgg aagattcctt tgctgaagga ttgctggatt
     9181 gcccgcacta tacgcggcct gaggtgttag aagggatgga agttccgcca gtgttactgt
     9241 cgggcaacca tgccgagata cgtcgctggc gtttgaaaca gtcgctgggc cgtacctggc
     9301 ttagaagacc tgaacttctg gaaaacctgg ctctgactga agagcaagca aggttgctgg
     9361 cggagttcaa aacggaacac gcacaacagc aacataaaca tgatgggatg gcgtaagccc
     9421 ccgagatatc agtttaccca ggataagaga ttaaattatg agcaatatta ttaagcaact
     9481 tgaacaagag cagatgaagc aggacgtacc ttccttccgt ccgggtgata ccgtggaagt
     9541 gaaagtatgg gttgttgaag gttccaaaaa acgtctgcag gcattcgagg gcgtggttat
     9601 cgctattcgt aaccgcggtc tgcactctgc attcactgtt cgtaaaattt ccaacggcga
     9661 aggcgttgag cgtgtcttcc agactcactc tccggtagtt gacagcattt ctgtcaaacg
     9721 tcgtggtgct gttcgtaaag ctaaactgta ctacctgcgt gagcgtactg gtaaggctgc
     9781 tcgtatcaaa gagcgtctta actaagattt cgcttaagcg acatcctgtt aagaagggct
     9841 ggccaattgg ctggcccttt ttttgtctgt ttgctcgtgt tttaaggggt tgtaatcacc
     9901 actgcgacgc ggcggttctc ggcgcggccc tgggcggtct tgttactggc tatgggatat
     9961 ttttttccta aaccctgtgt ggtgagattg ctgcgtggaa tttgtccacc aatagcccat
    10021 gcatcggcca cgacattcgc ccgtttcaat gataagcctt cgttgtaact gtcttcacca
    10081 tagttatcgg tgtgtccatc catacgggca tgtgttagcc ctgtcgaggc caatttggct
    10141 gccatggttt ggatctgttg ctggctttcc gggagcaatt tgtagtcatt ttttgcgaac
    10201 agaatggcat ctgataagcc cagcgaccag tcgccggcgg attcagtaaa tccataagat
    10261 tgcatagcgg cgacttgctc aggagtaaac tttccctgag gggactggca gccagtcagt
    10321 attagtgagg tgaaaatcag gggtgctacc aggtgcttta tcatatcgat atatccttgt
    10381 tatctcacca gcttttcggc acgctggtgt ttggcctgat acatattgtg atcggcaagc
    10441 tcttgtaatt tttccgcaga ggcgtgctca atggtcatcg cgtaaccaat gcttaatgtc
    10501 atggtggtct gatggccatt atgaagatca aacgggagat taaagatttg tgtcagtgct
    10561 gagcatatct gctgcacttc agattctgac tgtacatcat agagcaccat agcgaattca
    10621 tcgccgccca ggcggtatgc tttatgtcgc agcccgccaa cttcagctaa ccgttttgcg
    10681 atttcaatca agactctatc gcccgtcgca tgaccccagg tatcattgat gtatttgaaa
    10741 ttatcgccat caagaaatag taacgccgac gtttttcggg catcggaatt gttcatcaac
    10801 gtgttgatgc cgctacgaaa cgctgcgcgg ttagccagcc cggttaatgg gtcatgtagc
    10861 gcggtacgta gaagctgcgc atttttagcc tgtaaacgaa gctgccactc ttccatttca
    10921 tccagcagac tgttgaagtc gagagcgaag cggtgaaact cagcgatacg ttcttccgaa
    10981 actcgtcggg aaaaattgcg gttggaacgc acatcatgta cgacatcggt gatatttttc
    11041 agtgcttcca ccaggccatt gtgcaaatgg cgggtgaggg taattgcgat gcctgatgcc
    11101 agcagaatac aaccggtcag tacggcgagc gaaaaccaga taaaatggct gattgaactg
    11161 tcgcgagcgg ttaagcgtac ttcgccaatg gtttcaccat tgtgacgaat cggctgaatg
    11221 atgggggcgg ggaagagcca gtggcttatg aaattgctga aagtatcgcc tggatcctta
    11281 cgggtgtaat gccaggacgc cagaatattt tgctgcttat cacgtacttc tgcagttgaa
    11341 aattgccctt gctggcccag cgctgccagt gtttcagttg ctgcagggcc atcggcaaaa
    11401 acgacagctg cttccagact gtaagtca
//
LOCUS       DAESHI010000132        11295 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.134, whole genome shotgun sequence.
ACCESSION   DAESHI010000132 DAESHI010000000
VERSION     DAESHI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11295
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.134"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..741
                     /gene="hdhA"
                     /locus_tag="KW011_003603"
     CDS             <1..741
                     /gene="hdhA"
                     /locus_tag="KW011_003603"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HBH7661850.1"
                     /translation="DGKCAIITGAGAGIGKEIAITFATAGASVVVSDINADAANHVVD
                     EIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPKPFDMPMADF
                     RRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYASSKAAASHL
                     VRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRLGQPQDIANA
                     ALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            962..1552
                     /gene="uidR"
                     /locus_tag="KW011_003604"
     CDS             962..1552
                     /gene="uidR"
                     /locus_tag="KW011_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HBH7661851.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1941..3752
                     /gene="uidA"
                     /locus_tag="KW011_003605"
     CDS             1941..3752
                     /gene="uidA"
                     /locus_tag="KW011_003605"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBH7661852.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            3749..5122
                     /gene="uidB"
                     /locus_tag="KW011_003606"
     CDS             3749..5122
                     /gene="uidB"
                     /locus_tag="KW011_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HBH7661853.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            5161..6471
                     /gene="uidC"
                     /locus_tag="KW011_003607"
     CDS             5161..6471
                     /gene="uidC"
                     /locus_tag="KW011_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HBH7661854.1"
                     /translation="MRKIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
     gene            complement(6652..8160)
                     /locus_tag="KW011_003608"
     CDS             complement(6652..8160)
                     /locus_tag="KW011_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBH7661855.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8261..9436)
                     /gene="manA"
                     /locus_tag="KW011_003609"
     CDS             complement(8261..9436)
                     /gene="manA"
                     /locus_tag="KW011_003609"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HBH7661856.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            9635..11281
                     /gene="fumB"
                     /locus_tag="KW011_003610"
     CDS             9635..11281
                     /gene="fumB"
                     /locus_tag="KW011_003610"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBH7661857.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
BASE COUNT         2812 a         2650 c         2916 g         2917 t
ORIGIN      
        1 gacggaaaat gcgccatcat cacaggtgcg ggtgcaggta ttggtaaaga aatcgccatt
       61 acattcgcga cagctggcgc atctgtggtg gtcagtgata ttaacgccga cgcagctaac
      121 catgttgtag acgaaattca acaactgggt ggtcaggcat ttgcctgccg ttgtgatatt
      181 acttccgaac aggaactctc tgcactggca gactttgcta tcagtaagct gggtaaagtt
      241 gatattctgg ttaacaacgc cggtggcggt ggacctaaac cgtttgatat gccaatggcg
      301 gattttcgcc gtgcttatga actgaatgtg ttttcttttt tccatctgtc acaacttgtt
      361 gcgccagaaa tggaaaaaaa tggcggtggc gttattctga ccatcacttc tatggcggca
      421 gaaaataaaa atataaacat gacttcctat gcatcatcta aagctgcagc cagtcatctg
      481 gtcagaaata tggcgtttga cctgggtgaa aaaaatattc gggtaaatgg cattgcgccg
      541 ggggcaatat taaccgatgc cctgaaatcc gttattacac cagaaattga acaaaaaatg
      601 ttacagcaca cgccgatcag acgtctgggc caaccgcaag atattgctaa cgcagcgctg
      661 ttcctttgct cgcctgctgc gagctgggta agcggacaaa ttctcaccgt ctccggtggt
      721 ggggtacagg agctcaatta atacactaac ggaccggtaa acaaccgtgc gtgttgttta
      781 ccgggataaa ctcatcaacg tctctgctaa ataactggca gccaaatcac ggctattggt
      841 taaccaattt cagagtgaaa agtatacgaa tagagtgtgc cttcgcacta ttcaacagca
      901 atgataggcg ctcacctgac aacgcggtaa actagttatt cacgctaact ataatggttt
      961 aatgatggat aacatgcaga ctgaagcaca accgacacgg acccggatcc tcaatgctgc
     1021 cagagagatt ttttcagaaa atggatttca cagtgcctcg atgaaagcca tctgtaaatc
     1081 ttgcgccatt agtcccggga cgctctatca ccatttcatc tccaaagaag ccttgattca
     1141 ggcgattatc ttacaggacc aggagagggc gctggctcgt ttccgggaac cgattaaagg
     1201 gattcatttt gttgactata tggtcgagtc tattgtctct ctcacccatg aagcctttgg
     1261 gcaacgggcg ctggtggtcg aaattatggc ggaagggatg cgtaacccac aggtcgccgc
     1321 catgcttaaa aataagcata tgacgatcac ggaatttgtt gcccagcgga tgcgtgatgc
     1381 ccagcaaaaa ggcgagataa gcccagacat caacacgtca atgacttcac gtttactgct
     1441 ggatctgacc tacggtgtac tggccgatat cgaagcggaa gatctggcgc gtgaagcgtc
     1501 gtttgctcag ggattacgcg cgatgattgg cggtatctta accgcatcct gatcctctca
     1561 tttcgcgggc tggtgataac tgtgcccgcg tttcatactt taatttccct gtacaaaata
     1621 caatctagcc gggatcggag aattcccccc caaaaatatt caccgtagcc atatgtcatg
     1681 agagtttatc gttcccaata tgctcgaacg aacgttcggt tgcttatttt atggcttctg
     1741 tcaacgccgt tttaaagatt aatgcgatca atatcacgct gtgggtattg cagtttttgg
     1801 ttttttgatc gcggtgtcag ttctttttat ttccatttct cttccatggg tttctcacag
     1861 ataactgtgt gcaacacaga attggttaac taatcagatt aaaggttgac cagtattttt
     1921 atcttaatga ggagtccctt atgttacgtc ctgtagaaac cccaacccgt gaaatcaaaa
     1981 aactcgacgg cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt
     2041 ggtgggaaag cgcgttacaa gaaagccggg caattgctgt gccaggcagt tttaacgatc
     2101 agttcgccga tgcagatatt cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct
     2161 ttataccgaa aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt
     2221 acggcaaagt gtgggtcaat aatcaggaag tgatggagca tcagggcggc tatacgccat
     2281 ttgaagccga tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc accgtttgtg
     2341 tgaacaacga actgaactgg cagactatcc cgccgggaat ggtgattacc gacgaaaacg
     2401 gcaagaaaaa gcagtcttac ttccatgatt tctttaacta tgccgggatc catcgcagcg
     2461 taatgctcta caccacgccg aacacctggg tggacgatat caccgtggtg acgcatgtcg
     2521 cgcaagactg taaccacgcg tctgttgact ggcaggtggt ggccaatggt gatgtcagcg
     2581 ttgaactgcg tgatgcggat caacaggtgg ttgcaactgg acaaggcact agcgggactt
     2641 tgcaagtggt gaatccgcac ctctggcaac cgggtgaagg ttatctctat gaactgtgcg
     2701 tcacagccaa aagccagaca gagtgtgata tctacccgct tcgcgtcggc atccggtcag
     2761 tggcagtgaa gggcgaacag ttcctgatta accacaaacc gttctacttt actggctttg
     2821 gtcgtcatga agatgcggac ttgcgtggca aaggattcga taacgtgctg atggtgcacg
     2881 accacgcatt aatggactgg attggggcca actcctaccg tacctcgcat tacccttacg
     2941 ctgaagagat gctcgactgg gcagatgaac atggcatcgt ggtgattgat gaaactgctg
     3001 ctgtcggctt taacctctct ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac
     3061 tgtacagcga agaggcagtc aacggggaaa ctcagcaagc gcacttacag gcgattaaag
     3121 agctgatagc gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt gccaacgaac
     3181 cggatacccg tccgcaaggt gcacgggaat atttcgcgcc actggcggaa gcaacgcgta
     3241 aactcgaccc gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac gctcacaccg
     3301 ataccatcag cgatctcttt gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc
     3361 aaagcggcga tttggaaacg gcagagaagg tactggaaaa agaacttctg gcctggcagg
     3421 agaaactgca tcagccgatt atcatcaccg aatacggcgt ggatacgtta gccgggctgc
     3481 actcaatgta caccgacatg tggagtgaag agtatcagtg tgcatggctg gatatgtatc
     3541 accgcgtctt tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt
     3601 ttgcgacctc gcaaggcata ttgcgcgttg gcggtaacaa gaaggggatc ttcacccgcg
     3661 accgcaaacc gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc atgaacttcg
     3721 gtgaaaaacc gcagcaggga ggcaaacaat gaatcaacaa ctctcctggc gcaccatcgt
     3781 cggctacagc ctcggtgacg tcgccaataa cttcgccttc gcaatggggg tgctcttcct
     3841 gttgagttac tacaccgacg tcgctggcgt cggtgccgct gcggcgggca ccatgctgtt
     3901 actggtgcgg gtattcgatg ccttcgccga cgtctttgcc ggacgagtgg tggacagtgt
     3961 gaatacccgc tggggaaaat tccgcccgtt tttactcttc ggtactgcgc cgttaatgat
     4021 cttcagcgtg ctggtattct gggtaccgac cgactggagc catagccgca aagtagtgta
     4081 tgcatatttg acctacatgg gcctcgggct ttgctacagc ctggtgaata ttccttatgg
     4141 ttcacttgct accgcgatga cccaacaacc acaatcccgc gcccgtctgg gcgcggctcg
     4201 tgggattgcc gcttcattga cctttgtctg cctggcattt ctgatagggc cgagcattaa
     4261 gaactccagc ccggaagaga tggtgtcggt ataccatttc tggacgattg tgctggcgat
     4321 tgccggaatg gtgctttact tcatctgctt caaatcgacg cgtgagaatg tggtacgtat
     4381 cgttgcgcag ccgtcattga agatcagttt gcaaaccctg aaacggaatc gcccgctgtt
     4441 tatgttgtgc atcggtgcgc tgtgtgtgct gatttcgacc tttgcggtca gcgcctcgtc
     4501 gttgttctac gtgcgctatg tgttaaatga taccgggctg ttcactgtgc tggtactggt
     4561 gcaaaacctg gttggtactg tggcatcggc accgcttgtg ccggggatgg tcgcgaggat
     4621 cggtaaaaag aataccttcc tgattggcgc tttgctggga acctgcggtt atctgctatt
     4681 cttctgggtt tccgtctggt cactgccggt ggcgttggtt gcgttggcca tcgcttcaat
     4741 tggtcagggc gttactatga cagtgatgtg ggcactggaa gctgataccg tagaatacgg
     4801 tgaatacctg accggcgtgc gaattgaagg gctcacctat tcactattct catttacccg
     4861 taaatgcggt caggcaatcg gaggttcaat tcctgccttt attttggggt taagcggata
     4921 tattgccaat caggtgcaaa cgccggaagt tattatgggc atccgcacat caattgcctt
     4981 agtaccttgc ggatttatgc tactggcatt cgttattatc tggttttatc cgcttacgga
     5041 taaaaaattc aaagaaatcg tggttgaaat tgataatcgt aaaaaagtgc agcagcaatt
     5101 aatcagcgat atcactaatt aataatcaat aaaaataatc agaacatcaa aggtgcaact
     5161 atgagaaaaa tagtggccat ggccgttatt tgcctgacgg ctgcctctgg ccttatctct
     5221 gcttatgcgg cgcaactggc tgacgatgag gcgggactac gcatcagact gaaaaacgaa
     5281 ttgcgcaggg cggataagcc cagtgctggc gcgggaagag atatttacgc atgggtacag
     5341 ggaggattgc tcgatttcaa tagtggttat tattccaata ttattggcgt tgaaggcggg
     5401 gcatattatg tttataaatt aggtgctcgt gctgatatga gtacccggtg gtatcttgat
     5461 ggtgataaaa gttttggctt tgccctaggg gcagtaaaaa taaaacccag tgaaaatagc
     5521 ctgcttaaat caggtcgctt cgggacggat tatagttatg gtagcttacc ttatcgtatt
     5581 ccgttaatgg ctggcagttc gcaacgtaca ttaccgacag tttctgaagg agcattaggt
     5641 tattgggctt taacaccaaa tattgatctg tggggaatgt ggcgttcacg agtattttta
     5701 tggactgatt caacaaccgg tattcgtgat gaaggggtgt ataacagcca gacgggaaaa
     5761 tacgataaac atcgcgcacg ttctttttta gccgccagtt ggcatgatga taccagtcgc
     5821 tattctctgg gggcatcggt acagaaagat gtttccaatc agatacaaag tattctcgag
     5881 aaaagcatac cgctcgaccc gaattatacg ttgaaagggg agttgctcgg cttttacgcg
     5941 cagctcgaag gtttaagtcg taataccagc cagcccaatg aaacggcgtt ggttagtgga
     6001 caattgacct ggaatgcgcc gtggggaagc gtatttggca gtggtggtta tttgcgccat
     6061 gcaatgaatg gtgccgtggt ggataccgac attggctatc ccttttcatt aagtcttgat
     6121 cgtaaccgtg aaggaatgca gtcctggcaa ttgggcgtca actatcgttt aacgccgcaa
     6181 tttacactga catttgcaca gattgtgact cgcggctatg aatccagtaa acgagatgtg
     6241 cggattgaag gcgcgggtat cttaggtggt atgaactatc gggtcagcga agggccgtta
     6301 caagggatga atttctttct tgctgccgat aaagggcggg aaaagcgcga tggcagtacg
     6361 ctgggcgatc gcctgaatta ctgggatgtg aaaatgagta ttcagtatga ctttatgctg
     6421 aagtcagaaa taacgccgga gagaaaaacc tccggcgttt cagattgttg acaaagtgcg
     6481 cgttttttat gccggatgcg gcgtaaacgc cttatccagc ctacaaaaac tcataaattc
     6541 aaagtgttgc aggaaaaggt aggcctgata agcgtagcgc atcaggcaat ctctggtttg
     6601 ttttcagatg aaaacgccga agtgaaaatt ctccggcgtt ttggccgtga attactgctg
     6661 cggaattgcc ggtacagccg gaacgttaag agctggcatc gcaaacatac caacgaaatc
     6721 ttccagtggc attttctgcc cgtttaacgt tatctgaccg ttagtatatt gcaggctggt
     6781 ggtgatggta ttgtcctgca aggtggtcag acggaacatc tgccccattg ctgatgcacc
     6841 ttcaacttgc tgtttcgcca gttttttcgc ttgatcttcc tgataacctt ccagcttcgc
     6901 tacctgagtc ataaactcag ttgccatatc caccggaatg gtcagtttcg catccagaga
     6961 tttaaccgaa cgatctactt cctgcgccag cgtttgcggc gcttctttag tcgttgccgg
     7021 atctttcagg aacagcgaca gattcagcgc actttcaccc tgactgtttt tccagcttag
     7081 cggcgcgata gtaatcaccg gatcgccttt caacatcagc ggcagggcgc taaagaaggc
     7141 ttccgtcact ttctcctgat aaagttcggg attgttggca atttctggct gtgccagcag
     7201 cgcctgagtt tgcgcgttat attgctggct aaactgatgc caggcttcac catcaatctg
     7261 gccgactttt aaagtcagct tgccgctgcc cagatcctga ttctgtacct tcaggctgtt
     7321 tagcgagtaa tccagttggc tattgatcgt tttaccgtca ttgaccagat ccgatttacc
     7381 gctgatctcc atgccttcca gcagtgccag ttctttgcct tccactgaaa tggtcatttt
     7441 ttccagtgac agtttttggt ttcctacgcg ctcaccaaaa cttgccagcg tgctggaacc
     7501 gtcggttttc agattattaa aggtcaactg cactttctgg ttgtattcgt taactgcgtc
     7561 tatccgacca ctttgcgcct ccccggaaag ggagatggct ttgccgtctc tgtcagcatt
     7621 taactggaac tcgccgccgc taaaggcgac tttttcatcc ttttgctcgt aattcagtgg
     7681 cttgagcgaa atatcggaac tggaatcacc gctgtaacca atgcgcgagt taatctcaaa
     7741 aggcgtttca ccttttgcca tatcaaacag tggtttgctt acttcgttat taaccagcgt
     7801 ggtttgaatt gatgccatcg acgggatcag gttcagtttt ttaagctggg caagcgggaa
     7861 gggaccatga tcaaccgatt cgttgaagat gacgctctga ccgcttttaa tccacggatt
     7921 ttctttcccg gcaatgggtt tcaccaacag ttgcaactgg ctgctgaata cgccgcgatg
     7981 atagttttga taactcactt ccaggttgga ttcaggagct gtcagtttga gttgcgcgtt
     8041 cgcctgcgcg accatgtctt cgagatgggt ttcaatcttc ttgcctgtat accatgcgcc
     8101 gcctgtccag actacgccta gcgcaacaat gacgcctacc gctaccagcg atttattcat
     8161 aatgattatc cataaaatga aatcaggcgg actggccgcc tgaaggtgtt ataagccttt
     8221 aataagctta gcaagagatg ttaatttttt cagtaagctc ttacagcttg ttgtaaacac
     8281 gcgctaaacg gccgtggcct ttgacagtca ccggtgattc gttggcggca ataaacgctg
     8341 attcacccgg tttaagctgt aactgctgag aacctttcca caacgttgca tcgccttcga
     8401 cgcagaacaa aatggcggca ctctgctggc taatggtggt ttctttatca ctaaggtcat
     8461 gcagcgagaa ggcaaaatca tccactggaa tcgggaagtc cagttctgca ccttgtttca
     8521 ccggctgggt caacaactgg ttagccggtt tggcttcgaa tttcacattg gcaaccagtt
     8581 ccggaatatc aatgtactta ggcgtcagac ccgcacgcag cacgttatcg gagttcgcca
     8641 tcacttccag cgccacgcct tgcaggtaag cgtgcggtgt ttcagcgaac aggaacatcg
     8701 cttcgccagg attcaatttc accacattca gtaacagcgg ggagaacagg ccactgtctt
     8761 ccgggtaaaa ttcagaaatt aaacgaatcg tttgccacgg ttcaccctgc tgactgtcga
     8821 gcgccgattt taaaatcgcc agcgcgcggg atttttcttc accctgcata ttcaacaggc
     8881 tggcgaacag ttcgcttaaa cgttcggcat taggctgttg taaaaagtga gcaattgccg
     8941 gatgtgcacc tgcgactggc tggagtaggg agacaatctc ggaaaactca cgaaacgcgt
     9001 tcatcgcaag gaaaggcgtc agcgcaaaaa ccagctccgg cttgtggtta ggatctttat
     9061 agttacgctc ggcggcatcc atcgggatac ctgcggcatt ttctttggca aaaccgattt
     9121 cagaattgcg tttgtttgga tgaacctgaa tggagagcgg ctgtgctgcg cataatactt
     9181 tgaacaggaa aggcagttcg ccaaagcgtt tggcaacggc ctctccgagc agagtcgatt
     9241 tatcactctc aatcacatca cgcagtgaaa cgatatctcc ggcggcattc tgcactcgtg
     9301 aactgctttt cggatgtgcg cccatccaca gctcggccat cggctggctg gacggatttt
     9361 ccataccata aagttcagtc aacgccgttt tgctgcccca ggcatagttt tgcactgagt
     9421 taatgagttt ttgcatgatc aatccctgtt ttaatgtgga aattaatccc actattaaag
     9481 caagaatcct acgggaagta acctggagcc gcaaaaagtc gtactagtct cagtttttgt
     9541 taaaaaagtg tgtaggatat tgttactcgc ttttaacagg gcaacggaac acccgcccag
     9601 agcataacca aaccaggcag taagtgagag aacaatgtca aacaaaccct ttcattatca
     9661 ggctcctttt ccactcaaaa aagatgatac tgagtattac ctgctaacca gcgaacacgt
     9721 tagcgtatct gaatttgaag ggcaggagat tttgaaagtc gcacccgaag cgttaactct
     9781 gttggcgcgt caggcgtttc atgatgcgtc atttatgctg cgtccggctc accaacaaca
     9841 ggtggccgac attctgcgtg acccggaagc cagcgaaaat gataaatatg tggcgctgca
     9901 attcctgcgc aactccgaca tcgcggcgaa aggtgttctg ccaacctgtc aggataccgg
     9961 cactgcgatt attgttggta aaaaagggca gcgtgtatgg accggtggct gtgatgaagc
    10021 ggcgctggcg cgcggtgtct ataacactta tatcgaagat aatttgcgct actcgcaaaa
    10081 cgcgccgctg gatatgtata aagaggtgaa taccggcacc aatctgccag cgcagatcga
    10141 tctttatgcc gttgatggcg acgagtacaa attcctctgt atcgccaaag gtggtggttc
    10201 ggcaaacaag acgtatctct atcaggaaac caaagcgtta ctaacgccgg ggaaactgaa
    10261 aaattacctg gttgagaaga tgcgcacgct gggtacggcg gcctgtcctc cgtatcatat
    10321 tgcgttcgta attggtggaa cttctgcaga aactaacctt aaaacagtga aactggcttc
    10381 cgctaaatac tatgacgaac tgccaacgga agggaatgag cacggtcagg cattccgcga
    10441 tgtggaactg gaaaaagaat tgctgatcga agcgcaaaat ctcggtctgg gtgcgcagtt
    10501 tggtggtaaa tacttcgctc acgacatacg cgtgattcgc ctgccacgtc acggcgcatc
    10561 ctgtccggtt ggtatgggtg tttcctgctc tgctgaccgt aatatcaaag cgaagatcaa
    10621 ccgtcagggg atctggatcg aaaaactgga acataatcca ggtaaatata tccctgaaga
    10681 gctgcgtaaa gcgggagaag gcgaagcggt gcgcgttgac cttaaccgtc cgatgaaaga
    10741 gatcctcgca cagttgtcgc agtatcccgt ttctacacgc ttatcgctta acggcacgat
    10801 tatcgtcggt cgtgatattg ctcacgccaa actgaaagag cggatggata acggtgaagg
    10861 gctgccgcag tacattaaag atcatccgat ttactacgcg ggtccggcca aaacgccgga
    10921 aggttatgcc tccggttctc ttggcccaac gaccgccgga cggatggatt cttatgtcga
    10981 tcaactgcaa gcgcagggcg gaagtatgat catgctggcg aaaggcaacc gcagccagca
    11041 ggtgacggat gcctgtaaaa aacacggcgg cttctacctt ggcagtatcg gtggtccggc
    11101 cgctgtattg gcgcagggaa gtattaagag cctggaatgt gttgaatatc cggaactggg
    11161 aatggaagcc atctggaaaa ttgaagtgga agatttcccg gcgtttatcc ttgtggatga
    11221 taaaggaaat gacttcttcc agcagataca actcacacag tgcactcgct gtgtgaaata
    11281 aacagagccg ccctt
//
LOCUS       DAESHI010000133        11246 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.135, whole genome shotgun sequence.
ACCESSION   DAESHI010000133 DAESHI010000000
VERSION     DAESHI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11246
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.135"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..1760
                     /locus_tag="KW011_003611"
     CDS             <1..1760
                     /locus_tag="KW011_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HBH7661858.1"
                     /translation="VYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEI
                     ITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGY
                     WFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDY
                     PSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKD
                     NISKDEWENLHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHS
                     KHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYE
                     RDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGN
                     LFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWL
                     NKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVI
                     INKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIP
                     GRSALLLGKTGEPPNYLYL"
     gene            2096..3073
                     /gene="csiD"
                     /locus_tag="KW011_003612"
     CDS             2096..3073
                     /gene="csiD"
                     /locus_tag="KW011_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HBH7661859.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3093..4361
                     /gene="lhgO"
                     /locus_tag="KW011_003613"
     CDS             3093..4361
                     /gene="lhgO"
                     /locus_tag="KW011_003613"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HBH7661860.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4384..5832
                     /gene="gabD"
                     /locus_tag="KW011_003614"
     CDS             4384..5832
                     /gene="gabD"
                     /locus_tag="KW011_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HBH7661861.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            5846..7126
                     /gene="gabT"
                     /locus_tag="KW011_003615"
     CDS             5846..7126
                     /gene="gabT"
                     /locus_tag="KW011_003615"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HBH7661862.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            7437..8837
                     /gene="gabP"
                     /locus_tag="KW011_003616"
     CDS             7437..8837
                     /gene="gabP"
                     /locus_tag="KW011_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HBH7661863.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            8858..9520
                     /gene="csiR"
                     /locus_tag="KW011_003617"
     CDS             8858..9520
                     /gene="csiR"
                     /locus_tag="KW011_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HBH7661864.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9521..9970)
                     /gene="kbp"
                     /locus_tag="KW011_003618"
     CDS             complement(9521..9970)
                     /gene="kbp"
                     /locus_tag="KW011_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HBH7661865.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10054..10212)
                     /locus_tag="KW011_003619"
     CDS             complement(10054..10212)
                     /locus_tag="KW011_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HBH7661866.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10395..10694
                     /locus_tag="KW011_003620"
     CDS             10395..10694
                     /locus_tag="KW011_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH7661867.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10704..11228
                     /gene="ygaP"
                     /locus_tag="KW011_003621"
     CDS             10704..11228
                     /gene="ygaP"
                     /locus_tag="KW011_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HBH7661868.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
BASE COUNT         2786 a         2785 c         2939 g         2736 t
ORIGIN      
        1 atgtatataa tattaacgtt acgtcacctt ccggacagca agaaacattt aaaatactaa
       61 tcaatccaga acatctacgg cagacacttg agcgtaaatc tcttactgct gttcagaaat
      121 cacaatgtga aatcatcacc cctaaaaaac ccggcgaagc gattcttcat gcttttaatg
      181 ccacctacca gcaaatcagg gaaaatatgt ctgagtttgc acgttgccat tatgggtata
      241 tacaaatccc tccagtgaca actttccgcg ccgacggacc agaaactccc gaagaagaaa
      301 agggttactg gtttcatgct tatcaacccg aagatctttg taccatccat aatccaatgg
      361 gagatttgca ggattttatt gcattggtta aagatgctaa aaaatttggt atcgatatca
      421 ttcctgatta tacctttaac tttatgggaa ttgggggtag tggtaaaaat gacctggatt
      481 atccctctgc tgatatacga gcgaagatca gtaaagatat agaaggtggt atccctggct
      541 attggcaagg tcaggttttg attccattca ttaaagatcc agtaacaaaa gaacgtaaac
      601 aaatccatcc agaagatata catctcactg caaaagactt cgaagcaagt aaagataaca
      661 tctctaagga tgaatgggaa aacctccatg cattaaaaga aaagcgttta aatggaattc
      721 ctaaaacaac acccaaaagt gaccaggtta ttatgttgca aaatcaatac gttcgtgaaa
      781 tgcgaaaata tggcgtacga ggtttacgtt atgatgcggc aaaacactca aaacatgaac
      841 aaatagaaag atcaataacc ccaccgctta aaaattataa tgagcggtta cacaatacta
      901 acttatttaa cccaaaatat cataaaaaag ccgttatgaa ttacatggaa tatctggtaa
      961 cttgtcagtt ggatgaacaa caaatgtcat cactgcttta tgaaagagat gatttaagcg
     1021 ccattgattt ttcattgctc atgaagacga taaaagcctt ttcattcggg ggagatctcc
     1081 taacccttgc atcaaaaccg ggttccacaa tctcaagcat cccatcagaa agacggatat
     1141 tgattaacat taaccacgat tttcctaaca atggcaatct tttcaatgac tttctattta
     1201 accatcaaca agatgaacaa ttagcaatgg catatatggc cgctctcccg ttcagcagac
     1261 ctttagttta ctgggatggc caggtattaa aatcaacgac tgaaattaaa aattatgatg
     1321 ggtccacgcg tgtcggtggt gaggcgtggc ttaataaagg ttgctctacc tatcagcagc
     1381 tctacaatga attccacgca ttatatatag ataaagcagg aatatggagc gcatttgagg
     1441 gtgtatttgc aactaaaaac gttctggcct ttagtcgtgg ggattctgtg aacattaatc
     1501 actctcctca tgatggacta gttataataa ataaaggaaa cgaagaagtt gaaggtacct
     1561 ggcctaacaa attgcaacct ggaatatata aaaacatggg gagtaatagc gttaacatta
     1621 ttattaataa tacccgaaaa attatccccc ctggtaaagt atttacgctt agaggcggaa
     1681 ctctaaatat caatattcct gggcgtagcg ctcttctttt agggaaaaca ggagaaccgc
     1741 cgaactatct ctatttataa tttaatgtta tatctgcccc gataaaacgg ggcagataat
     1801 atgtttagtt tactaacggt cattttgcag tgaagccatt tactgttttt tatcgaccag
     1861 ataatctgtt ctctaatgtt aactccccct aacctgttgc tttagttatt catttcctgt
     1921 ctcactttgc cttaatactc tacgttaaat gttactaatt tgttgctttt gatcacaata
     1981 agaaaacaat atgtcgcttt tgtgcgcatt tttcagaaat gtagatattt ttagattatg
     2041 gctacgaaat gagcatcgcc atgtcaccct acatctcata agaggatcgc ttctgatgaa
     2101 tgcactgacc gccgtacaaa ataacgctgt cgattcaggc caggactata gcggattcac
     2161 tctcatcccg tcggcgcaat ccccgcgtct gctggaactc accttcaccg aacagacgac
     2221 caaacagttt ctcgagcagg ttgccgaatg gcccgtacag gcgctggagt acaaatcgtt
     2281 tctgcgtttt cgggtaggca aaattcttga cgatctgtgt gcgaatcagc tgcaaccatt
     2341 gctgttgaag accctgctaa accgtgctga aggtgcgctg ttgatcaatg cggtgggtat
     2401 cgatgatgtg gcgcaggcag atgagatggt gaagctggca acggcggtag cgcatctgat
     2461 tggtcgctca aatttcgatg cgatgagcgg tcagtattac gcgcgtttcg tggtgaaaaa
     2521 tgtcgataac tcagacagct atctgcgtca gccgcaccgc gtgatggaac tgcacaacga
     2581 cggcacgtat gtcgaagaga tcaccgatta cgtgctgatg atgaaaatcg atgagcaaaa
     2641 catgcagggt ggaaattcgt tgctgctgca tctcgatgac tgggaacatc tggaccacta
     2701 tttccgccat ccactggcgc gtcgcccgat gcgctttgcc gcgccgccga gcaaaaacgt
     2761 cagcaaagat gttttccatc cagtgttcga cgttgatcaa cagggtcgcc cggtgatgcg
     2821 ctatatcgac cagttcgtcc agccaaaaga ttttgaagaa ggcgtgtggt tgagcgagct
     2881 ttccgatgcc attgaaacca gcaaaggcat tctttctgta cccgttcccg ttggcaaatt
     2941 cctgttgata aacaacctgt tctggctgca cggtcgcgac cgctttactc cgcacccgga
     3001 tctgcgccgt gaactgatgc gtcagcgtgg ctatttcgct tacgccactc accactacca
     3061 gacgcatcag taagcgcaaa ggaattgagc ggatgtatga ttttgtgatt attggcggcg
     3121 gcatcatcgg catgtcgacc gccatgcaac tgattgatgt ttatccggat gcccgcattg
     3181 cgttgctgga aaaagagtcc ggcccggcct gtcaccagac gggccacaac agcggcgtga
     3241 tccatgccgg ggtctattac acgcccggca gcctgaaggc gcagttttgc ctggcgggga
     3301 accgcgccac caaagccttt tgcgatcaaa acggcattcg ctatgacaac tgcggcaaga
     3361 tgctggtcgc cacgtctcag ctcgaaatgg aacggatgcg cgccttatgg gaacgcacag
     3421 cggcgaacgg tatcgagcgc gagtggataa acgccgtgga actgcgcgag cgcgaaccga
     3481 atatcaccgg gcttggtggc atttttgtgc cgtccagcgg cattgtcagc taccgcgaag
     3541 tcacagcggc aatggcaaaa atcttccagg ccagaggcgg cgagattatt tataacgccg
     3601 aagtcagcgc cctcagtgag cataaaaacg gcgtggtgat acgcacccgt cagggcggtg
     3661 aatatgaagc atcgacgctg attagctgtt ccgggctgat ggctgaccgg ctggtgaaaa
     3721 tgctcggcct cgaaccgggc tttatcatct gcccgttccg tggtgagtat ttccgccttg
     3781 cgccggagca taaccagatt gttaaccacc tgatttaccc cattcccgac cccgcgatgc
     3841 catttttggg cgtgcatctc acccgcatga tcgacggcag cgtgaccgtc gggccaaacg
     3901 cggtgctggc tttcaaacgc gaaggctatc gcaagcgcga cttctcattt agtgacacgc
     3961 tggagatttt gagctcgtcg gggattcgcc gggtgctgca aaaccatcta cgctcaggac
     4021 tgggcgagat gaaaaactcg ctgtgcaaaa gcggctacct gcggctggtg caaaagtatt
     4081 gtccccggct ttcgttaagc gatctccagc cctggcccgc cggtgtgcgg gcgcaggcag
     4141 tatcgccgga cggcaagctg attgacgatt ttctgtttgt caccacccca cgcacgatcc
     4201 acacctgcaa tgcgccctcc ccggcagcga catcagcaat tcctattggt gcgcatattg
     4261 tcagcaaggt acaaacgctg ttggcaagcc agagtaaccc cggacgcacg ctgcgagcgg
     4321 cacgtagtgt ggatgcctta cacgccgcat ttaatcaata acctttgaaa acaggacgta
     4381 acaatgaaac ttaacgacag taacttattc cgccagcagg cgttgattaa cggggaatgg
     4441 ctggacgcca acaatggcgg gatcatcgac gtcaccaatc cggcgaacgg cgacaagctg
     4501 ggtagcgtgc cgaaaatggg cgctgatgaa acccgcgccg ctatcgacgc cgccaaccgc
     4561 gccctgcccg cctggcgtgc gctcaccgcc aaagaacgcg ccaacattct gcgcaactgg
     4621 ttcaatttga tgatggagca tcaggacgat ttagcgcgcc tgatgaccct cgaacagggc
     4681 aaaccactgg ccgaagcgaa aggcgaaatc agctacgctg cctcttttat tgagtggttt
     4741 gccgaagaag gcaaacgtat ttatggcgat accattcccg gtcatcaggc cgataagcgc
     4801 ctgattgtta tcaagcagcc gattggcgtt accgcagcta tcacgccgtg gaacttcccg
     4861 gcggcgatga ttacccgtaa agccggtccg gcgctggcgg caggctgcac catggtgctg
     4921 aagcccgcca gtcagacacc gttctctgcg ctggcgctgg cggagctggc gattcgcgcg
     4981 ggcattccgg ctggcgtgtt taacgtagtc accggttcgg caggcgcggt cggtaatgaa
     5041 ctgaccagca acccgctggt acgcaaactg tcgtttaccg gttcgaccga aattggccgc
     5101 cagttaatgg aacagtgcgc gaaagacatc aaaaaagtgt cgctggagct gggcggcaac
     5161 gcgccgttta tcgtctttga cgatgccgat ctcgataaag ccgtggaagg cgcgctggcc
     5221 tcgaaattcc gcaacgccgg gcaaacctgc gtctgcgcca accgtttata cgtgcaggac
     5281 ggcgtgtatg accgctttgc cgaaaaattg cagcaggcgg tgagcaaact gcacatcggc
     5341 gacgggctgg ataaaggcgt caccatcggg ccgctgatcg atgaaaaagc ggtagcaaaa
     5401 gtggaagagc atattgccga tgcgctggag aaaggtgcgc gtgtggtttg cggcggtaaa
     5461 gcggatgaac gcggcggcaa cttcttccag ccgaccattc tggtggacgt gccggccaac
     5521 gccaaagtgt cgaaagaaga gacgttcggc cccctcgccc cgctgttccg ctttaaagat
     5581 gaagccgatg tgattgcgca agccaatgac accgagtttg gccttgccgc ctatttctac
     5641 gcccgtgatt taagccgcgt cttccgcgtg ggcgaagcgc tggagtacgg catcgtcggc
     5701 atcaataccg gcattatttc caatgaagtg gccccgttcg gcggcatcaa agcctcgggt
     5761 ctgggtcgtg aaggttcgaa gtatggcatc gaagattact tagaaatcaa atatatgtgc
     5821 atcggtcttt aactggagaa tgcgaatgaa cagcaataaa gagttaatgc agcgccgcag
     5881 tcaggcgatt ccccgtggcg ttgggcaaat tcacccgatt ttcgctgacc gcgcggaaaa
     5941 ctgccgggtg tgggacgttg aaggccgtga gtatcttgat ttcgcgggcg ggattgcggt
     6001 gctcaatacc gggcacctgc atccgaaggt ggtggccgca gtggaagcgc agttgaaaaa
     6061 actgtcgcac acctgcttcc aggtgctggc ttacgagccg tatctggagc tgtgcgagat
     6121 tatgaatcag aaggtgccgg gcgatttcgc caagaaaacg ctgctggtta cgaccggttc
     6181 cgaagcggtg gaaaacgcgg taaaaatcgc ccgcgccgcc accaaacgta gcggcaccat
     6241 cgcttttagc ggcgcgtatc acgggcgcac gcattacacg ctggcgctga ccggcaaggt
     6301 gaatccgtac tctgcgggca tggggctgat gccgggtcat gtttatcgcg cgctttatcc
     6361 ttgcccgctg cacggcataa gcgaggatga cgctatcgcc agcatccacc ggatcttcaa
     6421 aaatgatgcc gcgccggaag atatcgccgc catcgtgatt gagccggttc agggcgaagg
     6481 cggtttctac gccgcgtcgc cagcctttat gcagcgttta cgcgctctgt gtgacgagca
     6541 cgggatcatg ctgattgccg atgaagtgca gagcggcgcg gggcgtaccg gcacgctgtt
     6601 tgcgatggag cagatgggcg tggcgccgga tcttaccacc tttgcgaaat cgatcgcagg
     6661 cggtttcccg ctggcgggcg tcaccgggcg cgcggaagtg atggacgctg ttgcaccggg
     6721 cggtctgggc ggcacctatg ccggtaaccc gattgcctgc gtggctgcgc tggaagtgtt
     6781 gaaggtgttc gagcatgaaa atctgctgca aaaagccaac gatctggggc agaagctaaa
     6841 agacggattg ctggcgatag ccgaaaaaca cccggagatc ggcgacgtac gcgggctggg
     6901 agcgatgatt gccatcgagc tgtttgaaga tggcgatcac agcaaaccgg acgcaaaact
     6961 caccgccgag atcgtggcac gcgcccgcga taagggcctg attcttctct cctgcggccc
     7021 gtattacaac gtgctgcgca tccttgtacc gctcaccatt gaagacgctc agatccgtca
     7081 gggtctggag atcatcagcc agtgttttgc tgaggcgaag cagtagctat gtccgtttgt
     7141 aagagttaac cgttgctaag ggaatgataa cgatgcagta aaaaataata acgatgaggc
     7201 gaacgacaaa gttaaaaacg actgcggaca gtcccctcgc ccctctggag agagggttag
     7261 ggtgaggggt aaaacacgcg gctaaaccct cccctggccc tctccctaag ggagagggag
     7321 aaaaccctcc cgctttcgtt tttaagattt tgttgaccgt ctcaattaaa cgcgtgaccc
     7381 ggcgcgggaa atgtcggggc gctctcccaa gtgacacact ttcgagagga ttcaggatgg
     7441 ggcaatcatc gcaaccacat gagttaggcg gcgggctgaa atcacgccac gtcaccatgt
     7501 tgtctattgc cggtgttatc ggcgcaagtc tgtttgtcgg ttccagcgtc gccatcgccg
     7561 aagcgggccc ggcggtatta ctggcctatc tgttcgccgg gctactggtg gttatgatta
     7621 tgcggatgtt ggcggaaatg gcggttgcca cgcccgatac cggttcgttt tccacctatg
     7681 ccgataaagc cattggtcgc tgggcgggct ataccatcgg ctggttgtac tggtggtttt
     7741 gggtactggt tatcccgctg gaagccaata tcgccgctat gatcctgcac tcgtgggttc
     7801 caggcattcc catctggtta ttttccctcg tcattaccct tgccttaact ggcagtaatt
     7861 tattaagcgt taaaaactac ggcgaatttg agttctggct ggcgctgtgc aaagtcatcg
     7921 ctatcctggc ctttattttc cttggtgcag tcgcaattag cggtttttac ccttatgccg
     7981 aagtgagcgg gatctcaaga ttgtgggata gcggcggctt tatgcccaac ggtttcggtg
     8041 cggtattaag cgcgatgttg atcaccatgt tctcgtttat gggcgcagaa attgtcacca
     8101 ttgccgccgc ggaatccgac acgccggaaa aacatattgt ccgcgccacc aactcggtta
     8161 tctggcgtat ttctatcttc tatttgtgct ctatttttgt cgtcgtggca ttaataccgt
     8221 ggaatatgcc ggggctgaaa gccgttggat cttatcgctc ggttctggaa ttgctcaata
     8281 ttccccatgc gaaattaatc atggactgtg tgatattact ttccgtaacc agttgcctga
     8341 actcggccct gtataccgcc tcaaggatgc tctactcctt aagccgtcgc ggtgatgctc
     8401 ccgcggtaat gggtaaaatc aaccgcagta aaaccccgta cgtggcggtg ttactctcca
     8461 ccggcgcggc attcttaacg gtggtggtga actattacgc gcctgcgaag gtatttaaat
     8521 ttctgatcga cagctccggc gctatcgccc tgctggttta tttagtcatc gccgtttcac
     8581 agttgcggat gcgcaaaatt ctgcgagcgg aaggaagcga aattcgcttg cgaatgtggc
     8641 tttatccctg gctcacctgg ctggtcatcg gctttattac ctttgtgttg gtagtgatgc
     8701 tattccgtcc ggcgcaacag ttagaagtga tctccaccgg cttattagcg atagggatta
     8761 tctgtaccgt gccaattatg gctcgctgga aaaagctggt attgtggcaa aaaacacccg
     8821 ttcataatac gcgctgataa tgatcaggag tcacaccatg accattacgt ctctggatgg
     8881 ctatcgctgg ctgaagaacg atattattcg cggtaatttt caaccggatg aaaaattacg
     8941 gatgagtttg ctgacatcgc gttatgcact tggcgttggg ccgttgcggg aagcactttc
     9001 tcaactggtg gcggaacggc tggtcacggt ggtgaatcaa aaagggtatc gggtggcgtc
     9061 tatgtcagag caggagctgc tcgatatttt cgatgcccgc gccaatatgg aagcgatgtt
     9121 agtgagtctg gcgattgccc gcggtggcga tgagtgggag gcagacgttc tcgcaaaagc
     9181 gcatctgctc agtaagctgg aagcctgtga cgccagcgag aaaatgcttg atgagtggga
     9241 tctgcgtcat caggcgtttc atacagcaat tgtggcgggc tgtggttctc actatttgct
     9301 gcaaatgcgt gaacggttgt ttgatctggc ggcgcgctat cgatttatct ggctgcggcg
     9361 aacggtgctt tcggtagaaa tgctggagga taaacacgat cagcaccaga ccctgactgc
     9421 ggcggtactg gcgcgagata ccgcgcgcgc cagtgagtta atgcgccagc atttactgac
     9481 gccaattccc attatccagc aggcgatggc tggcaattaa ctactcttcc ggaatacgca
     9541 acacttgccc cggataaatt ttatccgggc tttttagcat cggtttattc gcttcgaaga
     9601 ttttattgta cagattagcg ttaccgtaga cctgtttgga aatggcactc agagtgtcgc
     9661 cagacttaac ggtataaaac tggctggcag tggctggtgt cgccgttttc acctgatcat
     9721 caacactggc aataccggaa atattcccca ccgcaacaag gattttctcc ttcgcctcct
     9781 gactcaggcc gtcaccagtg accgtcgctt tgccgtcggc aatttgaatg ttcactttat
     9841 cagcatcagg tataccggtt ttgctcagat gctcctgcac cttcttcgcc tgatcgtctt
     9901 tatcgtgctg acctgtaacc gcgtcccaga gtttttctcc ggcatctttc acaaaattga
     9961 acagacccat agctacctca ttcgttaacg gaaacactca gaaagtgtag cagaggaacg
    10021 cggcgcgtta acgccgcggg ggtgagcaca ggtttaatcg cgggtttgca cccagaacgc
    10081 gtgaatcaaa ccggggatat acccaagcag tgtcagcaga atattaataa tgaacgccca
    10141 accgaaccct ttaccgagca gcacgccgag cggcggtaga atgatggtga tgacgattct
    10201 ccagaaaccc atatgtactc cctataagaa aattactcat tgtttaaaaa gagattttat
    10261 ctcttaagcg tagtaatttt agcggagcct gccagtttca gcttacgctt ttactctcat
    10321 ttacgctgct gaggctggta tatgttgcat ttttagttta gagcttactt aatttagaaa
    10381 atacttaaat aaatatgact gaactcgcgc aattacaggc cagtgccgaa caggcagcgg
    10441 ccttattgaa agcaatgagc catcctaaac gattgctgat tctgtgcatg cttagcggtt
    10501 cccccggcac cagcgcggga gagctgacgc gcattaccgg actgagtgcc tctgcgacat
    10561 cacagcatct cgctcgtatg cgggacgaag ggcttatcga cagccaacgg gatgcccaac
    10621 gcattctata ttccattaaa aatgaggcgg taaatgccat tatcgccacc ctgaaaaatg
    10681 tctattgtcc gtaaggagtc actatggctt tgacaaccat ttcgccgcat gatgcacaag
    10741 aattaatcgc acgcggcgca aagttaatcg atattcgtga cgctgatgaa tatcttcgtg
    10801 aacatattcc tgaagcagat ctggctccat tatccgtgct ggaacagtca ggtcttccac
    10861 ctaaattacg tcgcgagcaa attatattcc actgccaggc aggtaaacgc accagtaata
    10921 acgccgataa attagcagcg attgccgccc ccgcagaaat atttttactc gaagatggga
    10981 ttgatggctg gaaaagagct ggattgccag tagcggtaaa taaatctcaa cccttgccgt
    11041 taatgcgcca ggtgcagatc gctgcgggtg gtttaatatt aatcggcgtt gtactgggtt
    11101 ataccgtaaa tagcggtttc ttcttattaa gtggctttgt tggtgccggg ttactgtttg
    11161 caggaatcag cggtttttgt ggaatggcaa ggttgttaga taagatgccg tggaaccaac
    11221 gagcttgaga agcgacgccg gacgcg
//
LOCUS       DAESHI010000134        11244 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.136, whole genome shotgun sequence.
ACCESSION   DAESHI010000134 DAESHI010000000
VERSION     DAESHI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11244
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.136"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="KW011_003622"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="KW011_003622"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBH7661869.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="KW011_003623"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="KW011_003623"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HBH7661870.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3390)
                     /locus_tag="KW011_003624"
     CDS             complement(2698..3390)
                     /locus_tag="KW011_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBH7661871.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3520..5280
                     /locus_tag="KW011_003625"
     CDS             3520..5280
                     /locus_tag="KW011_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HBH7661872.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5686..6543
                     /gene="focA"
                     /locus_tag="KW011_003626"
     CDS             5686..6543
                     /gene="focA"
                     /locus_tag="KW011_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HBH7661873.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6598..8880
                     /gene="pflB"
                     /locus_tag="KW011_003627"
     CDS             6598..8880
                     /gene="pflB"
                     /locus_tag="KW011_003627"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBH7661874.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9072..9812
                     /gene="pflA"
                     /locus_tag="KW011_003628"
     CDS             9072..9812
                     /gene="pflA"
                     /locus_tag="KW011_003628"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HBH7661875.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(9894..10484)
                     /locus_tag="KW011_003629"
     CDS             complement(9894..10484)
                     /locus_tag="KW011_003629"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HBH7661876.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPVWWYSFPAMLKGYI
                     DRVWNNGLAYGDGQKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            10584..>11244
                     /locus_tag="KW011_003630"
     CDS             10584..>11244
                     /locus_tag="KW011_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH7661877.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKEQEIAVAGNI
                     IL"
BASE COUNT         2906 a         2822 c         2629 g         2887 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc caactgctcg aaataatccg gaaatgtttt ggccgtacat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg tggagtgata
      241 cgaatgtaat cgtgcccctc ttctacttcc gcaccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcgggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc cagaaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gttctttcat acgcggctca ccggtcagca caatatcatt gctacccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttacccc taacgctgtt aatgcattca gcatatggcg cacgtcatcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgccag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggcg ttataaatag aagcgcgcat
     1501 tccgccgacc acacggtgac ctttcagtgc atgaaggcca gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaatcacg ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtttcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc agcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt gcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgctttca tatgaatgat ttcactacaa cgaatacagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatctt caataataac
     3061 aaccggctta ccttccagta gatcttccag tttttcactg tgcgccatca accacattac
     3121 caggcggtat aacaacgcca gagtgataaa gacgataagt accggcacca tcggcacatc
     3181 atcataaaac gccacatctc ccgccgctga acccagcgtg agaatgatta acacttcaaa
     3241 cagcgacatc tgtcgcacac cgcgtcttcc ggtcattttg aggaacaaaa aaaccagtac
     3301 aaaggtataa agactacgta gtgcaacttc gccaaggaaa tcaaaaggca ctttatcaaa
     3361 tgccatacgg tggagatcga acgccttcat ttttatgctc ttatcgtggt ttatccgcta
     3421 aaagcatagt caacagttca tcggcatggc ggcggtgata gcgcctttga gataaaaccc
     3481 gtattattgc gcgctttccg tacgactaaa gtgattttca tgacgcaaac atttatcccc
     3541 ggcaaagatg ccgctctgga agattccatc gctcgcttcc agcaaaaact ttcagacctc
     3601 ggctttcaga ttgaagaggc ctcctggctg aatcctgtgc ctaacgtctg gtctgtacat
     3661 attcgcgaca aagagtgcgc actgtgtttt accaacggta aaggcgcaac caaaaaagcg
     3721 gcgctggctt ctgcacttgg tgaatatttc gagcgtctct caaccaacta cttttttgct
     3781 gatttctggc tgggcgaaac catcgccaac ggtccgttcg tgcattatcc caacgaaaaa
     3841 tggttcccac tgaccgaaaa tgacgatgtg ccagaaggac tactcgatga ccgtctgcgc
     3901 gcgttttatg atccggagaa tgaactgacc ggcagcatgc tgattgacct acaatccggt
     3961 aacgaagatc gtggtatttg cggcctgccg tttacgcgtc agtccgacaa tcagaccgtt
     4021 tatattccga tgaatatcat tggtaacctg tacgtctcca acggtatgtc cgcaggtaat
     4081 acccgcaacg aagcacgcgt tcagggattg tctgaagttt tcgaacgcta cgtgaaaaac
     4141 cgcattattg ctgaaagcat cagcctgcca gagatcccgg cagacgtgct ggcgcgttac
     4201 ccagcagtag ttgaagccat cgaaacgctg gaagcggagg gtttcccaat cttcgcttat
     4261 gacggttcgc ttggcggcca gtatccggtg atttgcgtgg tactgttcaa tcctgctaac
     4321 ggtacctgct ttgcctcttt cggtgcgcat cctgattttg gcgtagcact ggaacgtacc
     4381 gtgaccgagc tgctgcaagg tcgtggcctg aaagatttgg atgtgtttac tccgccaacc
     4441 ttcgatgatg aagaagtcgc tgaacatacc aacctcgaaa cgcactttat cgattccagc
     4501 ggtttaatct cctgggacct gttcaagcag gatgccgatt atccgtttgt ggactggaat
     4561 ttctccggca ccacggaaga agagtttgct acgctgatgg ctatcttcaa caaagaagat
     4621 aaagaagttt atattgccga ttacgagcat ctgggcgttt atgcttgccg tattatcgtg
     4681 cctggcatgt ccgatattta tccggctgaa gatctgtggc tcgcgaataa cagtatgggc
     4741 agccatttac gtgaaacgat tctttcgcta ccaggcagcg agtgggaaaa agaagattac
     4801 ctgaacctca tcgagcaact ggatgaagaa ggttttgatg actttacccg cgtgcgtgag
     4861 ctgttgggtc tggcgaccgg gtcggataac ggttggtaca ccctgcgtat cggtgaatta
     4921 aaagccatgc tggcgctggc tggtggcgat ctggaacagg ctctggtctg gaccgaatgg
     4981 acgatggagt ttaactcatc agtatttagt ccggaacgcg ccaactatta tcgctgcctg
     5041 caaacgttgt tattactggc acaggaagaa gatcgccagc cgctgcaata tctgaatgcg
     5101 tttgttcgca tgtacggcgc agatgccgta gaagccgcca gtgcggcaat gagcggcgaa
     5161 gcggcgtttt acggcctgca accggtagat agcgatctgc acgcgtttgc tgcacatcag
     5221 tcgttgttga aggcctacga aaagctgcag cgcgccaaag cagcattctg ggcaaaataa
     5281 aatcaaatag cctacgcaat gtaggcttaa tgattagtct gagttatatt acggggcgtt
     5341 tttttaatgc cccgctttac atatatttgc attaataaaa taattgtaat tataaggtta
     5401 aatatcggta atttgtattt aataaatacg atcgatattg ttactttatt cgcctgatgc
     5461 tcccttttaa ttaactgttt tagcggagga tgcggaaaaa attcaactca tttgttaatt
     5521 tttaaaattt atttttattt ggataatcaa atatttactc cgtatctgca taaaaaccat
     5581 gcgagttacg ggcctataag ccaggcgaga tatgatctat atcaatttct catctataat
     5641 gctttgttag tatctcgtcg ccgacttaat aaagagagag ttagtgtgaa agctgacaac
     5701 ccttttgatc ttttacttcc tgctgcaatg gccaaagtgg ccgaagaggc gggtgtctat
     5761 aaagcaacga aacatccgct taagactttc tatctggcga ttaccgccgg tgttttcatc
     5821 tcaatcgcat tcgtcttcta tatcacagca accactggca caggcacaat gcccttcggc
     5881 atggcaaaac tggttggcgg catttgcttc tctctggggc tgattctttg tgttgtctgc
     5941 ggagccgatc tctttacttc caccgtgttg attgttgttg ctaaggcgag tgggcgcatc
     6001 acctggggtc agttggcgaa aaactggcta aatgtctatt ttggcaacct ggtcggcgca
     6061 ctgctgtttg tacttttaat gtggctttcc ggcgagtata tgaccgcaaa tggtcaatgg
     6121 ggactaaacg tcctacaaac cgccgaccac aaagtgcacc atacttttat tgaggccgtc
     6181 tgtcttggta tcctggcaaa cctgatggta tgtctggcag tatggatgag ttattctggc
     6241 cgcagcctga tggacaaagc gttcattatg gtgctgccgg tcgcgatgtt tgttgccagc
     6301 ggttttgagc acagtatcgc aaacatgttt atgatcccga tgggtattgt aatccgcgac
     6361 ttcgcatccc cggaattttg gaccgcagtc ggttctgcac cggaaaattt ttctcacctg
     6421 accgtgatga atttcatcac tgataacctg attccggtta cgatcggcaa cattatcggt
     6481 ggtggtttgt tggttgggtt gacatactgg gtcatttacc tgcgtgaaaa cgaccaccat
     6541 taatggttgt cgaagtacgc agtaaataaa aaatccactt aagaaggtag gtgttacatg
     6601 tccgagctta atgaaaagtt agccacagcc tgggaaggtt ttaccaaagg tgactggcag
     6661 aatgaagtaa acgtccgtga cttcattcag aaaaactaca ctccgtacga gggtgacgag
     6721 tccttcctgg ctggcgctac tgaagcgacc accaccctgt gggacaaagt aatggaaggc
     6781 gttaaactgg aaaaccgcac tcacgcgcca gttgactttg acaccgctgt tgcttccacc
     6841 atcacctctc acgacgctgg ctacatcaac aagcagcttg agaaaatcgt tggtctgcag
     6901 actgaagctc cgctgaaacg tgctcttatc ccgttcggtg gtatcaaaat gatcgaaggt
     6961 tcctgcaaag cgtacaaccg cgaactggat ccgatgatca aaaaaatctt cactgaatac
     7021 cgtaaaactc acaaccaggg cgtgttcgac gtttacactc cggacatcct gcgttgccgt
     7081 aaatctggtg ttctgaccgg tctgccagat gcatatggcc gtggccgtat catcggtgac
     7141 taccgtcgcg ttgcgctgta cggtatcgac tacctgatga aagacaaact ggcacagttc
     7201 acttctctgc aggctgatct ggaaaacggc gtaaacttgg aacagactat ccgtctgcgc
     7261 gaagaaatcg ctgaacagca ccgcgctctg ggtcagatga aagaaatggc tgcgaaatac
     7321 ggctacgaca tctctggtcc ggctaccaac gctcaggaag ctatccagtg gacttacttc
     7381 ggctacctgg ctgctgttaa gtctcagaac ggtgctgcaa tgtccttcgg tcgtacctcc
     7441 accttcctgg atgtgtacat cgaacgtgac ctgaaagctg gcaagatcac cgaacaagaa
     7501 gcgcaggaaa tggttgacca cctggtcatg aaactgcgta tggttcgctt cctgcgtact
     7561 ccggaatacg atgaactgtt ctctggcgac ccgatctggg caaccgaatc tatcggtggt
     7621 atgggcctcg acggtcgtac cctggttacc aaaaacagct tccgtttcct gaacaccctg
     7681 tacaccatgg gtccgtctcc ggaaccgaac atgaccattc tgtggtctga aaaactgccg
     7741 ctgaacttca agaaattcgc cgctaaagtg tccatcgaca cctcttctct gcagtatgag
     7801 aacgatgacc tgatgcgtcc ggacttcaac aacgatgact acgctattgc ttgctgcgta
     7861 agcccgatga tcgttggtaa acaaatgcag ttcttcggtg cgcgtgcaaa cctggcgaaa
     7921 accatgctgt acgcaatcaa cggcggcgtt gacgaaaaac tgaaaatgca ggttggtccg
     7981 aagtctgaac cgatcaaagg cgatgtcctg aactatgatg aagtgatgga gcgcatggat
     8041 cacttcatgg actggctggc taaacagtac atcactgcac taaacatcat ccactacatg
     8101 cacgacaagt acagctacga agcctctctg atggcgctgc acgaccgtga cgttatccgc
     8161 accatggcgt gtggtatcgc tggtctgtcc gttgctgctg actccctgtc tgcaatcaaa
     8221 tatgcgaaag ttaaaccgat tcgtgacgaa gacggtctgg ctatcgactt cgaaatcgaa
     8281 ggcgaatacc cgcagtttgg taacaacgat ccgcgtgtag atgacctggc tgttgacctg
     8341 gtagaacgtt tcatgaagaa aattcagaaa ctgcacacct accgtgacgc tatcccgact
     8401 cagtctgttc tgaccatcac ttctaacgtt gtgtatggta agaaaacggg taacacccca
     8461 gacggtcgtc gtgctggcgc gccgttcgga ccgggtgcta acccgatgca cggtcgtgac
     8521 cagaaaggtg cagtagcctc tctgacttcc gttgctaaac tgccgtttgc ttacgctaaa
     8581 gatggtatct cctacacctt ctctatcgtt ccgaacgcac tgggtaaaga cgacgaagtt
     8641 cgtaagacca acctggctgg tctgatggat ggttacttcc accacgaagc atccatcgaa
     8701 ggtggtcagc acctgaacgt taacgtgatg aaccgtgaaa tgctgctcga cgcgatggaa
     8761 aacccggaaa aatatccgca gctgaccatc cgtgtatctg gctacgcagt acgtttcaac
     8821 tcgctgacta aagaacagca gcaggacgtt attactcgta ccttcactca atctatgtaa
     8881 ttagatttga ctgaaatcgt acagtaaaaa gcgtacaata aaggctccac gaaagtgggg
     8941 ccttttttag cacgagagcc ttttttgtca gctatctata ctctaaggtg actgccaaaa
     9001 cagactcgac gtagccttcg agctgcgcac caacacggcc tcagatgggc cacatctgga
     9061 gaaacaccgc aatgtcagtt attggtcgca ttcactcctt tgaatcctgt ggaaccgtag
     9121 acggcccggg tattcgcttt atcacctttt tccagggctg cctgatgcgc tgcctgtatt
     9181 gtcataaccg cgacacctgg gatacgcatg gcggtaaaga agttaccgtt gaagatttaa
     9241 tgaaagaggt ggtgacctat cgccacttta tgaacgcttc cggcggcggc gttaccgcat
     9301 ccggcggtga ggcaatccta caagctgagt ttgttcgtga ctggttccgc gcctgcaaaa
     9361 aagaaggcat tcatacctgt ctggacacca acggttttgt tcgtcgttac gatccggtga
     9421 ttgatgaact gctggaagta accgacctgg taatgctcga tctcaaacag atgaacgacg
     9481 agatccacca aaatctggtt ggagtttcca atcaccgcac gctggagttc gccaaatatc
     9541 tggcgaacaa aaatgtgaag gtgtggatcc gctacgttgt tgtcccaggc tggtctgacg
     9601 atgacgattc agcgcatcgc ctcggtgaat ttacccgtga tatgggcaac gttgagaaaa
     9661 tcgagcttct cccctaccac gagctgggca aacacaaatg ggtggcaatg ggtgaagagt
     9721 acaaactcga cggtgttaaa ccaccgaaga aagagaccat ggaacgcgtg aaaggcattc
     9781 ttgagcagta cggtcataag gtaatgttct aatttccccg taaagcggca actttattga
     9841 gttgccgctt ttttattccg cacttcattc tgctacagcg tttttaacac atcttatagt
     9901 gcatctacca tatcccgtac ctgagataat aacgactgat aatagctcgc gtgaagttct
     9961 tccccatcga ataccactgt attacacaag aaagtgacat cggcatcttc aataccaaga
    10021 taactgcaca tattttttaa ataatcgctt atattttttt cccagcccat ctggacaaat
    10081 gattctttgt ctcctccaac cagcgccacc caacgaactt tattgaatgg taatttctgc
    10141 ccatctccat aagccagccc attattccat actctgtcaa tatatccttt tagcattgcc
    10201 gggaagctgt accaccagac aggaaaaacc accactaacg tgtcatgttc aagcagctct
    10261 gaataaagct gatgaacctc tggagaataa cgtttatcca tattcttcca gtctggttca
    10321 tcttccggcg tcatcaccgg gttgaaatta cgccgatata aatcaagttc cgtcacctgt
    10381 attttccgct ccattgcccg ctgatggatc gcatcagcaa tatgtgcggt caatgaatca
    10441 tgacgaggat gggcccatac caaataaata cgttcagact gcataatttt acctccacgt
    10501 taagttctgg agcgcacttt acgcaggcgg cataatcatg taaacgtgat gattcaggat
    10561 gagttaatga aggattttca ctaatgcgga tgaatatgtc tgactttgcc actttctttg
    10621 ctgtggcccg taatcaaagc tttcgtgcag cgggcgatga gttaggctta tcctcgtccg
    10681 ccattagcca tagtattaaa acactggaac aacgtcttaa aattcgctta ttcaatcgga
    10741 caacccgaag cgtatcgtta acggaagcgg gttctaatct ctatgaacga ttgcgcccag
    10801 ctttcgacga aattcagatc atgttagatg agatgaatga ttttcgtctg acaccaacag
    10861 gaacgctcaa aatcaatgcc gcccgggtag ctgcacgtat atttttaatg cctttactgg
    10921 ttggtttcac acgtgaatat cctgatatta aagtcgagct cacaactgac gactctcttg
    10981 tcgatatcgt ccaacagggc tttgacgctg gcgttcgact tagtggcatc gtcgaaaaag
    11041 atatgatcag cgtggcgatc ggcccaccag tgaaattatg tgtagcagca acgccagagt
    11101 actttgcaag atatggcaaa ccccgccatc ctcatgatct gcttaatcat caatgtgttg
    11161 tatttcgtta tcccagcggc aaacctttcc actggcaatt tgcaaaggag caggaaattg
    11221 ctgtcgcagg gaatatcatt ctgg
//
LOCUS       DAESHI010000135        11242 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.137, whole genome shotgun sequence.
ACCESSION   DAESHI010000135 DAESHI010000000
VERSION     DAESHI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11242
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.137"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..309
                     /locus_tag="KW011_003631"
     CDS             <1..309
                     /locus_tag="KW011_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBH7661878.1"
                     /translation="VVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQF
                     FDQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN
                     "
     gene            508..789
                     /gene="ppiC"
                     /locus_tag="KW011_003632"
     CDS             508..789
                     /gene="ppiC"
                     /locus_tag="KW011_003632"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HBH7661879.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="KW011_003633"
     CDS             complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="KW011_003633"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBH7661880.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2501..3394
                     /gene="ilvY"
                     /locus_tag="KW011_003634"
     CDS             2501..3394
                     /gene="ilvY"
                     /locus_tag="KW011_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HBH7661881.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3446..4990)
                     /gene="ilvA"
                     /locus_tag="KW011_003635"
     CDS             complement(3446..4990)
                     /gene="ilvA"
                     /locus_tag="KW011_003635"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HBH7661882.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4993..6843)
                     /gene="ilvD"
                     /locus_tag="KW011_003636"
     CDS             complement(4993..6843)
                     /gene="ilvD"
                     /locus_tag="KW011_003636"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBH7661883.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6908..7837)
                     /gene="ilvE"
                     /locus_tag="KW011_003637"
     CDS             complement(6908..7837)
                     /gene="ilvE"
                     /locus_tag="KW011_003637"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HBH7661884.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7857..8120)
                     /gene="ilvM"
                     /locus_tag="KW011_003638"
     CDS             complement(7857..8120)
                     /gene="ilvM"
                     /locus_tag="KW011_003638"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HBH7661885.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8117..9763)
                     /locus_tag="KW011_003639"
     CDS             complement(8117..9763)
                     /locus_tag="KW011_003639"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HBH7661886.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9766..9816)
                     /locus_tag="KW011_003640"
     CDS             complement(9766..9816)
                     /locus_tag="KW011_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661887.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9903..10001)
                     /gene="ilvL"
                     /locus_tag="KW011_003641"
     CDS             complement(9903..10001)
                     /gene="ilvL"
                     /locus_tag="KW011_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HBH7661888.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10354..>11242
                     /locus_tag="KW011_003642"
     CDS             10354..>11242
                     /locus_tag="KW011_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBH7661889.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGRHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNG"
BASE COUNT         2554 a         3090 c         2951 g         2647 t
ORIGIN      
        1 gtcgtgctca acggaaaaat taatggcaac gcatgtatcg tcgttccatt atcaaccact
       61 cgcgatcatg acaagttaaa tcgtggaatg catgttgaaa tagccagtaa tgttatcaat
      121 gacctgcaat tctttgatca gcagattcgt tgggccaaag ccgacctagt acaacaagtc
      181 agtcgaaacc gattaaacag agcaaggact tacagagggt atcttaatca atgtttgcca
      241 catgagttgg ttgcagacat acagagggcg gtcatcaagt caatcaatgc catatcgctc
      301 ataaattgac aataaaataa ccatccccga taatgacttt gtagcccata gaggccctaa
      361 gttttctccc cttgagggac ctgcgaagcc cggattttcc gggcttttgc catatatgta
      421 ccagcccctc cctgactaac aattacgtta gcatctgcgg atcatgctaa aatcgccgcc
      481 tgacaattat tcactcaagg cacgatcatg gcaaaaacag cagcagcact gcatatcctt
      541 gtaaaagaag agaaactggc tctggatctt ctcgagcaga ttaagaacgg tgccgatttc
      601 ggcaagctgg cgaagaaaca ctccatttgc ccatcaggca aacgcggcgg tgatttaggt
      661 gaattccgcc agggtcagat ggttccggcg tttgataaag ttgtgttctc ttgtccggta
      721 ctggagccaa ccggcccgct gcacacccag ttcggttacc acatcattaa ggtgctgtac
      781 cgtaactaat agcaaggcct tctccaggag aaggccttga gtgttttctc cctctccctg
      841 tgggagaggg tcggggtgag ggcatcagcg cgcacttaac ccgcaactgc aatacgtttc
      901 atatccgtca tatagccgcg cagtttctta cctacctgct caatcgcatg gcagcgaatc
      961 gcttcgttca catcacgcag ttgcgcgtta tctaccgcac cttccggaat agctttaccc
     1021 agatcgcccg gttgcagctc tgccataaac ggtttcagca gcggcacaca agcgtaagag
     1081 aacagatagt taccgtactc ggcggtatca gagataacca cgttcatttc gtacagacgc
     1141 ttacgggcga tggtgttggc aatcagcggc agttcgtgca gtgattcata gtaagcagat
     1201 tcttcgatga tgccggaatc aaccatggtt tcaaacgcca gctcaacgcc cgctttcacc
     1261 atcgcgatca tcagtacgcc tttatcgaag tactcctgct cgccgatttt gccttcatac
     1321 tgcggcgcgg tttcgaaggc ggttttgccg gtctcttcac gccaggtcag cagtttctta
     1381 tcatcgttgg cccagtccgc catcataccg gaggagaatt cgccggagat gatgtcgtcc
     1441 atatgtttct ggaacagggg tgccatgatc tctttcagct gttcagacag cgcgtaagca
     1501 cgcagtttcg ccgggttaga gagacggtcc atcatcaggg tgatgccgcc ctgcttcagc
     1561 gcttcggtga tggtttccca accgaactga attagttttt ctgcgtatgc cggatcggtg
     1621 ccctcttcca ccagcttgtc gaagcacagc agagagccag cctgcaacat accgcacagg
     1681 atggtttgct cacccatcag gtcagatttc acttccgcaa cgaaggacga ttccagcacg
     1741 cccgcacggt gaccaccggt tgctgccgcc catgctttag cgatcgccat gccttcgcct
     1801 ttcggatcgt tttccgggtg aacggcaatc agcgtcggta cgccgaatcc acgtttgtac
     1861 tcttcacgta cttcggtgcc agggcatttc ggcgcaacca tgactacggt aatgtcttta
     1921 cggatctgct cgcccacttc gacgatgttg aaaccgtgcg agtaacccag cgccgcgccg
     1981 tctttcatca gtggctgtac ggtgcgcact acatcagagt gctgcttgtc tggcgtcagg
     2041 ttaaccacca gatccgcctg cgggatcagt tcttcgtaag taccaacttt aaaaccgttt
     2101 tcggtcgctt tacgccagga cgcgcgcttc tcggcaatcg cttctttacg cagagcgtag
     2161 gagatatcga gaccagaatc acgcatgttc aggccctggt tcagcccctg tgcgccacag
     2221 ccgacgataa ccactttttt accctgaagg tagctcgcgc cgtcggcgaa ttcgtcgcgc
     2281 cccataaagc gacatttgcc cagctgtgcc agctgctggc gcaggttcag tgtattgaag
     2341 tagttagcca tggtgattcc tcgtgatgtt gtgcttctta ttgttcggtt cgcgtttgcg
     2401 agattgaatt cactatatga caggaaattt attgcggaaa ttgatatatt cacaacgtca
     2461 cattgcaatt tctgcaacgt caacatcgag ggctgcgcca ttggatttac gcgatctgaa
     2521 aaccttcctg catctggcgg aaagccgcca ttttggccgc agcgcgcggg cgatgcacgt
     2581 tagcccatcc acgctctcac ggcagattca gcgcctggaa gaagatcttg gtcagccgct
     2641 atttgtgcgc gataaccgca cggtaacgct gacggaagcg ggcgaagagc tgcgcgtttt
     2701 cgcccagcaa acgctgttgc agtatcagca attgcgccac accatcgatc agcaagggcc
     2761 gtcgctctct ggcgaattac atatcttctg ctcggtgacc gctgcctaca gccatctgcc
     2821 gccgattctg gatcgcttcc gcgcggaaca cccgtcggtg gagatcaaac tcaccactgg
     2881 tgatgcggca gatgcaatgg aaaaagtggt cacgggagaa gcggatctgg cgattgccgg
     2941 gaaaccggag acgctacccg gcgctgtggc gttttcgatg ctggagaatc tggcagtagt
     3001 gctgattgcc cccgcgctgc cctgcccggt gcgtaatcag gtatctgtcg aaaagcccga
     3061 ctggtcaacg gtgccgttta ttatggccga tcaggggccg gtacgccgcc gcattgaact
     3121 gtggtttcga cgcaataaaa tcagcaaccc gatgatttac gccacggttg gcgggcatga
     3181 agcgatggta tcgatggtgg cgctcggctg tggtgtggcg ctgttgccgg aagtggtgct
     3241 ggaaaacagc ccggagcctg tacgcaaccg ggtaatgatt ttagatcgca gcgatgagaa
     3301 aacgccgttt gaactgggcg tgtgtgcaca aaaaaagcgg ctgcatgagc cgctgattga
     3361 ggcgttctgg aggattttgc cgaaccacaa atgacgttgt cgcgcgggta ggcctgataa
     3421 gcgaagcgct atcaggcatt tttccctaac ccgccaaaaa gaacctgaac gccggattat
     3481 tggtttcgtc gtggcaatcg tagcccagct catttagccg ggtttcgaaa tccggttcat
     3541 ggtcgccgag ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt
     3601 ggaacaaaga aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg
     3661 gtgattccgg gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc
     3721 cgaccatata gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt
     3781 agccgccgtc gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc
     3841 gcacgccgac aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga
     3901 ccgatcgccc gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg
     3961 tcaccgccaa caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca
     4021 ggccgtggaa gttcacgttg gcaccggaaa gaatatgcgc caggcgttcg ccgcgaatgt
     4081 tgtgctgagc gatatatttt ttcattcccg ccagcgccaa cgcgccagag ggttccgcca
     4141 ctgcgcgcac atcttcgaat aaatccttca tcgccgcaca gatcgcatca ctatcgacgg
     4201 tgatgatgtc gtcgagatac tcctggcata aacggaaggt ttcgtcgccg atgcgtttta
     4261 ccgcgacacc ttcggcgaac agtcccacgc gcggcagatc aaccggatga cccgcatcca
     4321 gcgctgcttt caggcaggcg gagtcttccg cttctacggc gatcactttg atttgtggca
     4381 tcagttgttt gatcagcacc gcaacgcccg ccgccagacc gccgccgccg actggcacaa
     4441 atacgcggtc gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg
     4501 caatcaccat cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt
     4561 cgatcgcttt agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga
     4621 atccacgtac cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct
     4681 tcacgcctaa ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag
     4741 tgatcacgcc gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc
     4801 gcagcttaaa gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa
     4861 gacgcgacga cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg
     4921 gcgcgcgcag cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg
     4981 agtcagccat tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct
     5041 ggttgccagg ctggcgtagg cgcgcagggc aaaggaaacc tgacgttcac ggtttttcgg
     5101 cgtccaggct ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc
     5161 gcttacctgt aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc
     5221 aatcagacca atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc
     5281 agaggtgccg ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat
     5341 tgatttcagg aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc
     5401 ttcatagcgg atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac
     5461 cgcgtcgtcc tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc
     5521 gacgcccgct gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag
     5581 gccgccgtct ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc
     5641 atcgagagaa tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg
     5701 gcctgcgcgg aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc
     5761 cagagtttgc ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc
     5821 gcgatccagt tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg
     5881 gtatttttgg gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc
     5941 gatatcactc atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac
     6001 cgtgttggta gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt
     6061 gctagcaata ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa
     6121 ttcaacaatg cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag
     6181 cagcgagccg ttgcctggct gcgacaaacc cagcgcttcg gtcaggcagt tcattgagtt
     6241 agcggtaaac atcccggagc aggaaccgca ggttgggcag gcggaacgtt caacctgatc
     6301 gctctgggag tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag
     6361 cttgatgatc tgctcggaca gtttggtttt cccggcttcc atcgggccgc cggaaacaaa
     6421 gatcaccgga atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca
     6481 gttagagatg cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc
     6541 agcgatcagt tcgcgagatg gcagtgaata aagcatcccc ccgtggccca tagcaatccc
     6601 atcatccacc gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc
     6661 ggcgaccagt ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga
     6721 gttcacaacc gcgataatcg gcttgccaaa atcggcatcg gtcattccgg tggcgcgcca
     6781 cagcgcacga gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg
     6841 catactttat ttactcccag tgtctgtctc gtaaatggga cggtaagtgc cgtcccattt
     6901 tttgtattta ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga
     6961 agaggccgaa gaaggcttgc tgaatgcatt tggtaaccgg gccgcaacgg ccttcaccca
     7021 cctgaatacc gtctacactg cgcactggcg tgatttctgc cgccgtaccg gacataaaca
     7081 cttcatccgc caggtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca
     7141 gctctttcgc cagtttgatg atggcatcac gggtaatacc cggcagcgcg gatgaggtaa
     7201 acggtggggt gaacagcacg ccgtctttca cttcaaacag gttttcacct gcgccttcag
     7261 agatgtaacc gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc
     7321 tacccaccag cagggaagag aggtagttac caccggcttt tgccgccgtc gggatggtgt
     7381 ttggtgctgc gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg
     7441 cgcccagata cgctccccac gggaaagcag cgataatcac gtcggttgag tatcccgctg
     7501 gcgggtttac tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg
     7561 tgagattatt tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgcg
     7621 aaaccgggaa gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc
     7681 ggaatacaac cggtcctttg tgcgagtcgt agcaacggat gccttcaaaa accgaagtgc
     7741 catagtgcag cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc
     7801 cattgaacca aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag
     7861 gcgcggattt gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc
     7921 agtttattta actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg
     7981 atatttatat tttgtgcatc gctggcggcg gccatattca ttgagcagac gtggaaacca
     8041 cgatgacgca ccacgcgtaa aacacgttct aaggtttctg gattgaagcg agccgataca
     8101 ttgacctgat gttgcatcat gataatttct ccaacatttc tgaattactg gcgccaggcg
     8161 gcaccagcgg ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat
     8221 cactgttcag catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc
     8281 cagggatgcc gaaggcgctg gctaacatga ggaaatcggg gttatcagta agggtggttt
     8341 cgctgtatcg ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt
     8401 tatcgagtaa gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca
     8461 cattcatcat gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga
     8521 cttgtgcgcc aaccgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga
     8581 tgaaattttc cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca
     8641 catctgtggt cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg
     8701 gcgcgtagat agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg
     8761 cgcagtattg ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat
     8821 ttaaatcacc ctgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga
     8881 tatccatatg gataacactg gcgtgtggtg cgaaagtatt cagtttgccg gtcacccggt
     8941 catcaaaacg tgcgcccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg
     9001 ctttgatgcc gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc
     9061 ccagcccttt cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag
     9121 ccggaactgc ctgcgccata cccacgccac cgccaacgta cagcatcggt ttttgcgctt
     9181 ttgccagcat ctggcgcgct tgctcaactt cggcatgtgg gaaagtcact tcgttttcaa
     9241 cggtggtgaa ccacggttcc aggtcaccgc tggctaactg gatatctttt gggatatcga
     9301 ccagaaccgg accaggacga cctgagcagg caacgtcgaa tgcttcagcc atgatgcgcg
     9361 gcaactcttc cagcgactgc accagaaagc tgtgcttggt acaggctaac gacaatccta
     9421 ggacatccac ttcctgaaat gcgtcagtgc cgataaacgg tgcggacact tgaccggtga
     9481 tggcaacaac agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg
     9541 cgcccggacc agacgtggcg atacatacgc cagttttgcc ggtagcacgg gcataaccga
     9601 tagccgccat tgccgcaccc tgctcatgtc ggcatagcaa gtgctccacg ccgccgtcat
     9661 acaatgcatc gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac
     9721 cctgtgcccg caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct
     9781 gaatcttgag aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt
     9841 aaggtcaaaa aaaacccccg gacctttcgg tgcgggggtc ttagttcgtt aaggcttgat
     9901 ctctaagcct ttcctcgtcc aagtgcagcc ccgcacggtg ggataataat caccaccacg
     9961 ctaatcacga ctaggctaat cactcgtaga agggctgtca ttttgtcttt tcttgcatct
    10021 tgttcgaagg aatgcctaaa gagttaccat agattttgcg aatagcacaa gatatttttg
    10081 ggcctatttt tatgatagct ttaataattt caatagaagt tgttgttttt taaaaagaaa
    10141 atagtgagtg aaaaaaattc aatttcagta taaatgttgt tcccttcctc gtagttcatc
    10201 ctttgtccgc tgcatttttg cgagcatcat tccaccgcca gaacaactca acttcacttg
    10261 cacaaataaa caggaatcag ccactaaaat tcacgaatta gcgggctaca gcagcgatat
    10321 cttatgcaca taatggtgac tcaaggaggg cttatgtcac tgtcaattgt tcatacccgc
    10381 gcagccctgg gagtaaatgc gcccccgatc actgttgagg tacatatcag taaaggtcta
    10441 cccggcttaa cgatggtggg cttaccagaa acaactgtaa aagaagctcg cgatcgcgtg
    10501 cgcagcgcca ttatcaatag cggatatgaa tatccggcga aaaaaatcac catcaacctt
    10561 gctccagccg atctgccaaa agaaggggga cgatatgatt tacctatcgc cattgcgttg
    10621 ctggcggcct cagaacagct tacagccaat aagttagatg aatatgaatt agtcggagaa
    10681 ctggctctta caggcgctct gcgtggcgtt cccggtgcaa tctccagtgc aactgaagct
    10741 attaagtcgg gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt ggggctaatt
    10801 aacggtgaag gatgcctgat agccgatcat ctgcaagctg tctgtgcgtt tctggaaggt
    10861 aagcacgctc tcgaacgccc gaaaccaact gatgcagtat cccgggcgct acaacatgat
    10921 ctcagtgatg ttgtcggtca ggaacaagga aagcgaggac tggaaattac cgccgctggc
    10981 aggcacaacc ttttactgat tgggccgccg ggaacaggta aaacaatgct cgccagccgt
    11041 attaatggcc tgttgccaga tttaagcaat gaagaggcac tggagagcgc tgcgatatta
    11101 agtctggtaa atgctgaatc agtacaaaaa caatggcggc agcgcccgtt ccgctcacct
    11161 catcacagtg cgtcgttaac tgcgatggtg ggcggtggtg caattccagg gccaggtgaa
    11221 atttcgttgg cgcataacgg cg
//
LOCUS       DAESHI010000136        10939 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.138, whole genome shotgun sequence.
ACCESSION   DAESHI010000136 DAESHI010000000
VERSION     DAESHI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.138"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            108..605
                     /gene="ftnA"
                     /locus_tag="KW011_003643"
     CDS             108..605
                     /gene="ftnA"
                     /locus_tag="KW011_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HBH7661890.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(643..882)
                     /locus_tag="KW011_003644"
     CDS             complement(643..882)
                     /locus_tag="KW011_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HBH7661891.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            1073..2284
                     /gene="tyrP"
                     /locus_tag="KW011_003645"
     CDS             1073..2284
                     /gene="tyrP"
                     /locus_tag="KW011_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HBH7661892.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2346..3011)
                     /gene="yecA"
                     /locus_tag="KW011_003646"
     CDS             complement(2346..3011)
                     /gene="yecA"
                     /locus_tag="KW011_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HBH7661893.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3207..3293)
                     /locus_tag="KW011_003647"
     tRNA            complement(3207..3293)
                     /locus_tag="KW011_003647"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3257..3259),aa:Leu,seq:taa)
     gene            complement(3306..3379)
                     /locus_tag="KW011_003648"
     tRNA            complement(3306..3379)
                     /locus_tag="KW011_003648"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3345..3347),aa:Cys,seq:gca)
     gene            complement(3434..3509)
                     /locus_tag="KW011_003649"
     tRNA            complement(3434..3509)
                     /locus_tag="KW011_003649"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3474..3476),aa:Gly,seq:gcc)
     gene            complement(3661..4209)
                     /gene="pgsA"
                     /locus_tag="KW011_003650"
     CDS             complement(3661..4209)
                     /gene="pgsA"
                     /locus_tag="KW011_003650"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBH7661894.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4266..6098)
                     /gene="uvrC"
                     /locus_tag="KW011_003651"
     CDS             complement(4266..6098)
                     /gene="uvrC"
                     /locus_tag="KW011_003651"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBH7661895.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6095..6751)
                     /gene="uvrY"
                     /locus_tag="KW011_003652"
     CDS             complement(6095..6751)
                     /gene="uvrY"
                     /locus_tag="KW011_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HBH7661896.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            7047..7223
                     /locus_tag="KW011_003653"
     CDS             7047..7223
                     /locus_tag="KW011_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661897.1"
                     /translation="MIKIFIGHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            7210..7434
                     /locus_tag="KW011_003654"
     CDS             7210..7434
                     /locus_tag="KW011_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HBH7661898.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7501..8223)
                     /gene="sdiA"
                     /locus_tag="KW011_003655"
     CDS             complement(7501..8223)
                     /gene="sdiA"
                     /locus_tag="KW011_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HBH7661899.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(8453..9205)
                     /gene="yecC"
                     /locus_tag="KW011_003656"
     CDS             complement(8453..9205)
                     /gene="yecC"
                     /locus_tag="KW011_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HBH7661900.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(9202..9870)
                     /gene="tcyL"
                     /locus_tag="KW011_003657"
     CDS             complement(9202..9870)
                     /gene="tcyL"
                     /locus_tag="KW011_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HBH7661901.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(9885..10871)
                     /gene="dcyD"
                     /locus_tag="KW011_003658"
     CDS             complement(9885..10871)
                     /gene="dcyD"
                     /locus_tag="KW011_003658"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HBH7661902.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
BASE COUNT         2713 a         2838 c         2578 g         2810 t
ORIGIN      
        1 attcgtattt ttaataattg aagtttatat tttacctttt gcaaataata aaataacaaa
       61 ttatagtgac gccacagcaa caaatataac ctttgtggag cactatcatg ctgaaaccag
      121 aaatgattga aaaacttaat gagcagatga acctggaact gtactcttca ctgctttatc
      181 agcaaatgag cgcctggtgc agctatcata ccttcgaagg tgctgccgcg ttcctgcgcc
      241 gtcacgccca ggaagagatg acgcatatgc agcgtctgtt tgattacctg actgataccg
      301 gcaatttacc gcgtattaat accgttgaat ctccgtttgc tgaatattcc tcacttgatg
      361 aattattcca ggaaacctat aaacacgaac aattaatcac ccagaaaatt aacgaactgg
      421 ctcatgctgc aatgaccaat caggactacc caacatttaa tttcctgcag tggtatgttt
      481 ctgagcagca tgaagaagag aaactgttca aatcgattat tgataaatta agcttggcag
      541 gcaaaagcgg cgaaggtctg tattttatcg acaaagaact ctctaccctc gacacacaaa
      601 actaatgctc tccggcggca ggtttactgc cgccgtttca aatcagtgac ggcaaatctt
      661 actctgatcg gtcgaaaaac cgtctttcga aggtataaat ttgccttttg ctgccagaaa
      721 cgctaccagc tctcccgctg tcatcccttc tgccgagcag gtgtgaaaac gtgcctgttc
      781 accaaaacgc gctttaatcg cagcttccag actggcatgc gtatattgct cgcctgattc
      841 aatcatcata tttaacactt catgaccgtg aatagagtcc atcgttcctc ctcaaaaaaa
      901 gcctagcgta gcgattgccg cttatgaaga ctttgcgcca gcgcaggact gaatgctttt
      961 tattgtacat ttatatttac actatatgta acgtcggttt gacgaagcag ccgttatgcc
     1021 ttaacctgcg ccgcagatat cactcataaa gatcgtcagg acagaagaaa gcgtgaaaaa
     1081 cagaaccctg ggaagtgttt ttatcgtggc gggaaccaca attggcgcag gcatgctggc
     1141 aatgccgctg gctgcggccg gtgttggttt tagcgttacg ttaatcttgt tgattgggct
     1201 ttgggcgttg atgtgctaca cggcgctatt actgctggag gtgtaccagc atgttccggc
     1261 agataccggt ctgggcacgc tggcaaaacg ctatctggga cgctacggtc aatggctgac
     1321 gggcttcagt atgatgttct taatgtatgc tctgactgcg gcatacatca gcggtgtcgg
     1381 tgaattgttg gcctccagca tcagcgactg gacaggtatt tcaatgtcgg caaccgctgg
     1441 cgtgctgttg ttcacttttg ttgccggtgg cgtggtttgt gttggaacat cgctggtcga
     1501 tttatttaac cgttttctgt tcagcgccaa aattattttt ctggtggtga tgctggtatt
     1561 gctgctaccg catattcaca aagtgaatct tttaaccctg ccgttgcaac aggggctggc
     1621 tctgtcagca atcccggtga tttttacctc atttggtttt cacggtagcg tgccgagtat
     1681 tgtcagctat atggatggca acgttcgtaa gctacgctgg gtgtttataa ccggtagtgc
     1741 gatccccctg gtggcatata ttttctggca ggtggcaacg cttggcagca ttgattcaac
     1801 aacctttatg ggattgctgg ctaatcatgc tggattaaac gggctgttac aggcgttacg
     1861 cgaaatggtg gcctctccgc atgttgagct ggcagtgcat ttatttgctg atttagccct
     1921 cgccacgtca tttctcggcg ttgcgttagg cttatttgat tatctggctg atttatttca
     1981 gcgttcaaat accgttggtg gacggttgca aactggtgca attacctttc tgccgccgtt
     2041 ggcgtttgca ctgttttatc cacgaggatt tgtgatggcg ctgggttacg ccggtgtggc
     2101 gctggcggta ctggcattga ttatcccttc gctgttgacc tggcaaagca gaaagcacaa
     2161 tcctcaggcg ggttaccggg tcaaaggtgg tcgtccggcg ctggtggtgg tgtttctctg
     2221 tggtattgct gtgattggcg tgcaattttt gattgcggca gggttgttac cagaagtggg
     2281 gtgatcagat agcctcaaat tccttattgg gtgccagaat taacgctgac acccaatttg
     2341 gcctcttaat gcaggcagca ctgcttaaat ttcttaccac taccgcacgg gcaaggatcg
     2401 ttacgccccg gtttctcttc tgctttgatc ggttgctgaa cagctttttc ctgcggatgc
     2461 gccatccagt acgcatgtag atcaagcgcc gccagtcgaa tggcatctac gctctcttca
     2521 aacgcttctg gcgacatctt ttctacccgc tcgaagtttt cctcagtacc gtgcagcgca
     2581 atcgcctcca gcgctggttt taacgaatcg ggcaacgttg accagtcaga aagtgccacg
     2641 ccccgcatat agccaaagca ccactcctca acaatcgtca gctcgctgcc atcaacttct
     2701 cgcaagccga ataacggctc aaactgctcc gggaattcgt tcagacgctc tgcggtatcg
     2761 gccatatgtt gaaaagccag gttcataaag cgcgtcatct ctttctctga cgcccagcgc
     2821 ggcacatagt cagccccacc ccacacggca accagccact gttccggttc aatctcttgc
     2881 ggagaactca acaccgccgt caataaaccg tccagctccg ccacatcaag gatggcgtgg
     2941 ttagtgttgt atttggtcag aatatcgtcc agccattcca actcactttc gtttaacggt
     3001 cccgttttca tacgcttttc cttgtggatc tcaactcgcc agcacctatc ttacatgccg
     3061 gtccgtatca gagatacttt ttgagtggct ttgccggtga ttaaaaatta aggagggtgt
     3121 aacgacaagt tgcaggcaca aaaaaaccac ccgaaggtgg tttcacgaca ctgcttattg
     3181 ctttgatttt attcttatct ttcccatggt acccggagcg ggacttgaac ccgcacagcg
     3241 cgaacgccga gggattttaa atcccttgtg tctaccgatt ccaccatccg ggctcgggaa
     3301 gaaagtggag gcgcgttccg gagtcgaacc ggactagacg gatttgcaat ccgctacata
     3361 accgctttgt taacgcgcca aattcttcag gcctttcagc cagacatccg cttgacgccg
     3421 atgtctttta aactggagcg ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg
     3481 tcgtgctcta ccaactgagc tattcccgca ttcatcaagc aatcagttaa tcacttgatt
     3541 ttattatcgt ctggcaatca gtgccgccgt tcgatgcgtt gcattctact tacctggcgc
     3601 gatgagtcaa cgatattttt caccactttt gatcgtttgc tgaaaattac gccgaaacga
     3661 tcactgatca agcaaatctg cacgcgcagc gctcaaatat tgcaacattg accacagagt
     3721 cagtaccgca gccacaaaga aaagtgcaat accggcgtac tcaacccaaa tgttcggacg
     3781 ccacagcagc catgccaacg ccaccatctg ggcagtggtt ttcactttcc caatccagga
     3841 gacagccacg ctactgcgtt tacccaactc cgccatccat tcgcgtagcg cagaaataat
     3901 aatttcacgg gcgatcatcg ttgctgccgg taaggtcacc caccagctgt ggtaatgctc
     3961 ggttaccagc accatggcga tagccacgag aactttatct gctacagggt caaggaaagc
     4021 accaaaccgg gtactctggt tccagcggcg tgccagaaaa ccatcgaacc agtcagtcac
     4081 cgccgcgacg cagaaaatga gcgcggcggc aaacggcgac caggtgacag gcagataaaa
     4141 gaccaataca aagaatggga taaggatgac acggaacagt gtaagcaacg tagggatatt
     4201 aaattgcata atgacgggta actatctgtt gtcagtaaga ttacccctat gttgctacag
     4261 agacatcaat gtttcaacga ccagaagatc ttttctgcca gaccttgcga aatacccggc
     4321 acttttgcaa tttcctcgac gctggcgtta cgtaaacctt gcaaaccgcc catatatttc
     4381 aacaacattt gccgacgttt tggcccgacg ccttcaatgg tttccaggga actggtattt
     4441 ttgaccttcg cccgtttttt acggtgcccg ccaatcgcgt gatcatgtga ttcatcgcga
     4501 atatgctgga taacatgcag cgcgggagaa tctggcggca aactgaatcc ctcaccttcc
     4561 ggctcaaaga acagcgtttc cagcccagcc ttacgatctg ctcctttggc aacgccaagt
     4621 agcagcggat gatttttatc ccatgagaca tccagttcgg cgaagacatt tttcgcctgc
     4681 gcaagctggc ctttgccgcc gtcgataagg atcacatccg ggatcttact gtcgtcaatg
     4741 gctttaccat aacgccgacg cagcacctga ttcatcgccg cataatcatc gcccggcgtg
     4801 atgcctgtaa tgttatagcg ccgatactcc gcacgcagcg ggccgttagc atcaaacacc
     4861 acacaggaag cgacggtttg ttcgcccatg gtatggctga tgtcaaagca ctccatccgc
     4921 ttcacttccg gcaatttcaa cacgctggca agcgcggtca gtcgctggtg aacggtagat
     4981 tgctgcgaaa gtttgctggt taaggccgtc gccgcattgg tgcgcgcgag tttcagataa
     5041 cgcgccctat cgccgcgagg tttggtttga acattaatct tgcgtcccgc cagttctgaa
     5101 agggaatcgg cgagcagcgt tttatcgcta agattaaaat cgagcaggat ctcacccggt
     5161 aaggtgcgca tctggctgcc ttgtaaatag aactggccta cgaaggtttc taccacctcg
     5221 ctcagttccg taccgccagg cactttcggg aaatagctgc ggctgccgag cactttgccc
     5281 tgacgaatga acaatacgtg gacacaagcc atgcccgcat cgaacgccac accaataacg
     5341 tcgaggtcgt cgccggtatt ggaaacgaat tgtttttcgg tgacgcgtcg caccgcctga
     5401 atttggtcgc gaatacgtgc agcttcttca aactccagat tctggctggc agtttccata
     5461 cgactaatga gttgcgtaag cacctgatca tctttgccag acaaaaacag gcgcacatac
     5521 tcaacctgct gagcgtattc ttcttcactc accagtcctt caacgcacgg tcccagacag
     5581 cgccctatct gatattgcag acacggacgc gagcgattgc gataaacact attttcgcac
     5641 tggcgaatgg ggaaaatctt ttgcagtagc gccagtgttt cacgtacggc atagccattc
     5701 gggaacgggc cgaaatattc acctttggca tgcttcgcac cacgatgcat cgccagacgc
     5761 gggtgggtat cgccactcag gaagataaaa ggataagatt tatcatcgcg tagcaaaacg
     5821 ttgtaacgcg gctgatagag tttgatgtag ttgtgttcca gcaacagcgc ttcggtttct
     5881 gtatgcgtaa ccgttacatc aatttgctgg atctgggcga ccagcgcttc ggttttgcgc
     5941 gaagcgaggt tgctgcggaa atagctggaa agccgttttt tcaggtcttt cgctttgccg
     6001 acatagataa ccgtaccacc agcatcgtac atgcgataaa cgcctggctg gctggttacg
     6061 gtttttaaaa acgcttttgc gtcaaactga tcactcactg acttgataat gtctccgcat
     6121 tacacagacc atggcgaatt gccaggtgag tcagctcaac atcgccatga atgtttagtt
     6181 tactgaacat acgatagcgg tagctgttca ccgttttcgg actgagattg agctgttctg
     6241 agatctcatt gaccttctgg cccttggtga tcatcagcat aatctgcaat tcacgttcag
     6301 acaaactggc aaatgggctt tctgtttttt ctggttcgat ctggcttaac gccatttgtt
     6361 gagcgatatc agaagcaatg taacgctgcc ctgaatagac agaacgaatc gcactcacga
     6421 cttcctgcgg agccgcgcct ttgctgaggt agcccgcagc accggcctgc atgactttcg
     6481 ctggtaaagg gttttctgta tggacggtaa gcatgatgat tttgacatca gctgtggaac
     6541 gcgcgatttt acgcgtcgcc tcaagaccgc caatgcccgg catactcatg tccattagca
     6601 ccacgtcaac ggcatttgtc cggcaccact taacggcgtc ttcaccgcac gatgcctcac
     6661 cgacgacttt tatacccttt atatcttcca gaatgcgtcg tatccctgcg cgcaccagtt
     6721 cgtggtcatc aacaagtaga acgttgatca aaggaatatc tccagaaata gggataacgc
     6781 tactgatagt tagtcattcg tatattagcg gtttttattg caactttgaa acgttaaaaa
     6841 tggtgggtat tcgatttttc tctcgttttt gtaaattcga ttgtccacag ttatggtcac
     6901 gcccgtcacc aacggcttcc gggatcgttt cacagtaagc ccggcgatga aatgtgacaa
     6961 aaattgactt tattcagcaa aaatgaaaat cagcccgctg aatatgtaac attaattaac
     7021 ccgaacaata cgggcaaaaa acattcatga taaaaatatt tattggtcat tatattaacg
     7081 catttaatag cattgttgac gtcacgctca aaaaacaacc actgctattt ttagcaaagc
     7141 ttatggtata ctccgccgcc ttaacatttt tcaccgcaaa ttttcattgc aacatgacga
     7201 ggaaaataaa tgagtacgcc tgatttttct actgccgaga ataatcaaga actggcaaat
     7261 gaagtctcct gcctgaaagc gatgctgacg ctgatgctac aggcgatggg acaagctgac
     7321 gcgggccgcg tgatgttaaa gatggaaaaa cagcttgcgc tgatcgaaga cgaaacccag
     7381 gctgcagtat tttccaaaac ggttaagcaa attaaacaag cctaccgtca gtaatataaa
     7441 accggctgag agcgtgcctt tcagccgatt tttgcatctg gcacgcagaa agaaaagcga
     7501 tcaaattaag ccagtagcgg ccgcgtaaca ggccacctgg gtcttatttg gtgcattgat
     7561 ttttttctgc atgtttttct gatggaaatt gaccgtattc tcagagattg acaaaatcat
     7621 cgctatctct gctgatgtct tcccttccgc cgtccatttc agaatttctt tttcgcgctt
     7681 gctgaaattc atctctggcg tcatcactat ttcatcattt aaacgcatca gagccatcag
     7741 actttcgcgc accagtaact gcatttttaa ttgcagttca tcactaagaa tgggtatttc
     7801 gcgcgtgctg caacgggaaa aggacaaaaa gcccagcgcc cgatttggca gcattaaata
     7861 ctgagtgaca ccgcggcgta aaccatgtgc gtgcgcggct tcccataatg gctgtgcttc
     7921 gctgaataag tcatcattcc acattaaatg gccctgacta aagttttcag ggttcagcac
     7981 aggatcaatt gcgagaaaat tttttgcctg ataataacta acccacgact cagggtaatt
     8041 ggtgtagaaa gccactttag gtcgcgtgaa tggtaccggg tggcgaacac ataacgaata
     8101 gtaatcgtac tccagctgct gagcctgaag ctctatttca tggtagacct cttctgcggc
     8161 ctccatcctc tgaaaacgca acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg
     8221 catagtaaac cgcaacgccc ctgagagtga gtatcattta taatgataat tcaaactatc
     8281 acataaataa gatttatata taatttatat tattcaggca atgaattact tttgcaagcc
     8341 atcgcattct cttatgttat taatgagtta tgctgatttg ttaagcagtt ttatcacgct
     8401 tgaaatggcg tcccgccccg acaggtgaat cgtcggggct gattttttct tattattgca
     8461 gcagaaactt ctcgaggaac tggcgggtgc gaggctgctg gggatcagca aataacgctt
     8521 ttgctgcccc ctgctcgact atccgcccct ggtccataaa aatcgcccgg tccgcaacat
     8581 cccgggcaaa gctcatttcg tgcgtcacaa tcaccatcgt gcgcttttcc tgcgccagct
     8641 ggcggattgt gttcaggact tcgcccacca gctctggatc cagcgctgac gttggctcgt
     8701 caaacaaaat cacctcagga cgcattgcca gcgcacgcgc aatcgctaca cgctgctgtt
     8761 gaccgccaga caaacgacgt ggatagctgg tttctttacc ggccagccca actttcgcca
     8821 gcagctcgcg agcgcgcgcc gtggcctctt ctttcggttc gcctttgacg atcaccggcc
     8881 cttcaataat gttttccagc accgtacgat gcggaaacaa attaaagttc tggaagacaa
     8941 acccgacgtg ctgacgcaac tggcggatca gcgatttttg ctgacttaat gaacgtgcag
     9001 tatcaatagt gatatcgcca acggtgatcg ttcccgcttc cggttgttcc agcagattta
     9061 tgctgcgtag caacgtggtt ttgccggaac cactcggacc gataattgcc accacttcgc
     9121 caggctttac ctcgaggtcg ataccgtgca gcaccgtctg accgtgaaat tttttcacca
     9181 ggttcttaac ttcaatggca ctcattttgg ttctctctcc tggcgattaa gttgattctc
     9241 aaaatggttc tgcaacgtcg ataatactgt cgccatgatc cagtagatga gcgaagccgc
     9301 caggtacatg gtgaacacct ccagcgtgcg cgaggtaatc aactgcgcct gacggaacag
     9361 ctccggcacc tggattgtcg cggccagcga cgtatccttt accaggctga taaagctgtt
     9421 cgacagcggc ggcaacgcca cccgcgccgc ctgtggcaaa atcgcgcgac gcatagtctg
     9481 ccatggcgtc ataccaatac tggcagctgc ttcccactga cctttatcaa tagaagaaat
     9541 ggcggcgcgc agtgtttcgg cggcataggc ggcagtgttc agcgacagac caatcattgc
     9601 tgacggaatg ggatctaatt cgataccaaa ctgcggcagg ccgtaataga tcataaacag
     9661 ttgtgcgatg agcggtgtac cacgaaaaat agagatataa aaacgcgcca gccagcgcac
     9721 gggccagatt ggcgacaggc gcatcagcgc gagaataaac ccgagcagta agccaaaaaa
     9781 catgccgccg atactgagtt gcagcgtata cccagcccct ttcagcagga acggcaagga
     9841 atcaataacc agttgtatac tttcttgcat gagcgttccc ggagctaaac gtggggatga
     9901 taggcgaaca gcgcaggcgc accgccggta tgaataaaca gaatcggccc ttcatctttg
     9961 aagcgtttct gactgataca gccaatcagc cccgccatcg cttttccggt atacacagga
    10021 tcaagcagaa tgccttccag ccgcgccagc aatttcactg cttccatgcc ttcgtcgttc
    10081 ggcacgccgt agccaggtgc aaaatagtca tcccagagta taatttccgc tgatgcggtc
    10141 agctccagtt ctttcgcaat cgcctgttgt aggttaacca ctttcggcaa ctgatcggca
    10201 acggaacgcg acacggtcac gccaatcagt tcgctttcag gcatcaggtg ttccagccca
    10261 acagccagtc cggcgtgagt tccggcactg cccgatgcga ctaccaccga cgaaatatta
    10321 accgcccctt cacactgttg cgcgatttcc agcgcactct ccacataacc tagcgcgcca
    10381 agagcattag aaccgccaac cggaatgaca tacgggcgaa agccttgtgc ttcgactcgc
    10441 gtcgccagct cttccagttg ggcattggga tcggtcagtg cttcgcacat ttcaatctgg
    10501 gtattgaaca gatccagcaa caagcgattg ccgttggtta aatagttttc tgcggttgtg
    10561 ccaataggat tttccagcag cgccacacag tgcagaccga gtttcgccgc aactgcggca
    10621 gtctgccgca catggttaga ctggatcacc ccggcagtaa tcagcgtatc ggcaccttcg
    10681 cgcagagcat ctgccgcgag aaattctagc ttacgtaatt tattgccgcc cattgccatg
    10741 ggggtgacgt catcccgttt gatgaaaatt tcccgtccta gataatcaga aaagcgcggc
    10801 agatattcga gcggcgttgg cgcgccgata aactccaacc gtggaaaacg ggttaaatta
    10861 tgcagtggca taacagcctc cgatgtatgt tgttgtgatt ttcttattat gcacgctgaa
    10921 aacgcgtaaa taaaaaagg
//
LOCUS       DAESHI010000137        10833 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.139, whole genome shotgun sequence.
ACCESSION   DAESHI010000137 DAESHI010000000
VERSION     DAESHI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10833
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.139"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..277)
                     /locus_tag="KW011_003659"
     CDS             complement(<1..277)
                     /locus_tag="KW011_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH7661903.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRS"
     gene            complement(444..1184)
                     /locus_tag="KW011_003660"
     CDS             complement(444..1184)
                     /locus_tag="KW011_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HBH7661904.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1277..1912)
                     /gene="argO"
                     /locus_tag="KW011_003661"
     CDS             complement(1277..1912)
                     /gene="argO"
                     /locus_tag="KW011_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HBH7661905.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(2051..2911)
                     /gene="mscS"
                     /locus_tag="KW011_003662"
     CDS             complement(2051..2911)
                     /gene="mscS"
                     /locus_tag="KW011_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HBH7661906.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(3102..4181)
                     /gene="fbaA"
                     /locus_tag="KW011_003663"
     CDS             complement(3102..4181)
                     /gene="fbaA"
                     /locus_tag="KW011_003663"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HBH7661907.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4396..5559)
                     /gene="pgk"
                     /locus_tag="KW011_003664"
     CDS             complement(4396..5559)
                     /gene="pgk"
                     /locus_tag="KW011_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBH7661908.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5609..6628)
                     /gene="epd"
                     /locus_tag="KW011_003665"
     CDS             complement(5609..6628)
                     /gene="epd"
                     /locus_tag="KW011_003665"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HBH7661909.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7000..7431
                     /locus_tag="KW011_003666"
     CDS             7000..7431
                     /locus_tag="KW011_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBH7661910.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7454..8032
                     /locus_tag="KW011_003667"
     CDS             7454..8032
                     /locus_tag="KW011_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBH7661911.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8033..8740
                     /locus_tag="KW011_003668"
     CDS             8033..8740
                     /locus_tag="KW011_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBH7661912.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8728..9405
                     /locus_tag="KW011_003669"
     CDS             8728..9405
                     /locus_tag="KW011_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBH7661913.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9399..10076
                     /locus_tag="KW011_003670"
     CDS             9399..10076
                     /locus_tag="KW011_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBH7661914.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(10048..10761)
                     /locus_tag="KW011_003671"
     CDS             complement(10048..10761)
                     /locus_tag="KW011_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HBH7661915.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10758..>10833)
                     /locus_tag="KW011_003672"
     CDS             complement(10758..>10833)
                     /locus_tag="KW011_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH7661916.1"
                     /translation="ARFSGMVKTALTLAVTTTLKELTP"
BASE COUNT         2580 a         2879 c         2699 g         2675 t
ORIGIN      
        1 gtgaacgacg ccctgagtta tggatttcat tatcaatcgc agaaagtcgt tgataaaccg
       61 ggaacagttt ttgatacagt tcctgaccgg ctttattgag gctgatatta ttatctttgc
      121 gggtaaatag cgtatagcca atttgttctt ccagcgcggc aatgctttta ccaaatggcg
      181 aagccgtcat atggattttt tctgccgccc gggcaatatt attggtttgc gctaataaaa
      241 tgaaattgcg catctttttc gaaataaaaa tgtccatccc ccctccccgc atccttgtcc
      301 gcttaaccgt ttcagtcagc ctatccttga tgaaaccgcg agcaaagata ggtgattacg
      361 tcatggtttt acagaaaatt acagaaaaag gaggcaatat cgggtaaagg cattagcccg
      421 acgaatacgt cgggctacaa gtattattgt gctgcaggtg ttttagcggg ttgttgatcc
      481 acaggttcta actggaagac cacatcgacc tgatcatcaa actgaatagc ggcctgctcg
      541 taagtttcct gggcagacac cggcgcggca tcggctttca tcatccgcac cattgggctg
      601 ggctgatagt tggaaacatg gtagcgcacg ctatataccg gccccagttt acgatgaaag
      661 ccgttcgcca gttcctgcgc ctgatgaatc gcgttatcaa tcgctgcctt acgcgctttg
      721 tctttatagg catccggctg cgccacgccc agcgacacag aacgaatttc gttaagaccc
      781 gccttcagcg cgccatccag caaggaattc agtttgtcta actgacggag cgtgacttcc
      841 accgttctca cagcgcggta gcctttcagg atacttttac catcctgata atcataatct
      901 ggctgggtgc gtaagttcgc tgagctgata tcttttttcg cgatctgatt gagttcaagg
      961 aaggaaatgt attgtgcgac gcgctcatct gcctgtttct tggctgtagc ggcatccttc
     1021 gcggccacgt taacttcaat cgcaagagtg gcaatgtctg gcaccgcatc cacacttgcc
     1081 gtaccggagg tgacaatatg cggtccatcc ggcaattcgt tagcctgcgc tgccatcccg
     1141 ctaataccca ttaatgccgc cagggcgata actttgaact tcacgttagt ctctccgtgt
     1201 taattgactt gcccaaaatt caggacctct ggaggcaagc ttagcgcctc tgttttattt
     1261 ttccatcaga tagcgcctaa ctgaacaagg cttgtgcatg agcaataccg tctctcgcca
     1321 gctgcaaggc tataaaccac atgacacatc ccactaccag attgataatg cgctgtgatt
     1381 ttgccgtgcg caggcgcggt gccagccagg ctgcgagaat agccagacca aagaaccaca
     1441 ggaaagaggc gctaattgtc ccgagcgcaa accaacgttt tggttcgacg tcaagctgcc
     1501 cgccaaggct gcccagtaca acaaaagtat ccaggtaaac atgcggattc agccaggtca
     1561 ctgccagcat ggtggcgata attttccatc tgccttgctt caggacttcg gcactagcta
     1621 actcaatatt actactcatc gctgttttaa aagcgccaaa accataccac agcaagaagg
     1681 ctacgccgcc ccaggtgacc agcgccagca accacggcga ctgcatcaat aacgcgctgc
     1741 caccaaaaat cccggcgcaa atcaggacca aatcgctgat agcacaaagt aaggcaatca
     1801 taatgtggta ctgacgacga atgccctgat tcatcacaaa agcattttgt ggaccgagcg
     1861 gcagaatcat agctgcccca agagcaatac cttgaaagta ataagaaaac acgttggata
     1921 ttccgaatta agtatctgat gcagagacta tatcgcggga ttgttattag cggaaattga
     1981 taatattaat tggcaatcag aataactaat aagagagagg ccagagagta ttatctggcc
     2041 tcagcgttga ttacgcagct ttgtcttctt tcacccgctt aaagttcaca tccatttgcg
     2101 ggtacgggaa gctgataccg gcggcatcaa attcacgttt aatacgctcc agcacatccc
     2161 agtacacgtt ttgcagatcg ccgctgttgc tccagacgcg gaccacgaaa ttaatcgacg
     2221 atgcaccaag ttcgttcaga cgcacagtca tttcgcgatc tttcaaaatg cgatcttcag
     2281 actggataat attggtcagg atctgcttaa cctgatcgat atcggaatca tacgccacgc
     2341 caataataaa ttcgttacgg cgaactggct cgcgagagaa gttaataata tttccggcaa
     2401 taattttacc gttcggaata acgataattt taccgtctgc agtacgcatg gtggtggaga
     2461 aaatctgcac actcagcaca gtaccggcta cgccgccgag atcaacatat tctccggcac
     2521 ggaacgggcg gaacatgaca agtaacacgc cagcggccag gttagaaagt gacccctgca
     2581 aagccagacc aacagctaag cctgcggcac cgagtacagc aatgactgac gctgtttgta
     2641 cacccacgcg tcccagggca gcgattagcg taaaggcgat aataccgtaa cggactaatg
     2701 cagaaagaaa atcagcaaca gtggcatcga ttttacggga gatcatcagg cgattcaccg
     2761 cgttggaaat catccgcgcg ataatcaaac caacgatgat gatcgcgagt gccgccacga
     2821 tgtttactgc ataacttagc agcagcgcct ggttagctac cagccagctt cccgcgccgt
     2881 ttatgctatc gacaacattc aaatcttcca ttcaatattc cttttcaact gactcaaaat
     2941 ggagaaatac actccgccgc cttatgacgg gcagtctgac aagggtaaac aataaagcat
     3001 aattttgcca aatagatcac agatttctca tctgaatcac cttgcttttg gcgaaaaaaa
     3061 gacccgcaga gcgggccttg agataagcag aaaggaatat cttacagaac gtcgatcgcg
     3121 ttcagttcct ggaatgcttt ttccagacga gcgatcatcg aagtctgacc ggcacgcagc
     3181 catacgcgcg gatcgtagta tttcttgttc ggctgatctt cgcctttcgg gttacccaac
     3241 tgcccctgca gataagcttc gttcgctttg tagtagttca gaacgccttc ccaggttgcc
     3301 cattgggtat cggtatcgat gttcattttt actacgccgt agcttacgga gtctttgatt
     3361 tcctgagcag tagaaccgga accaccgtgg aatacgaagt tcaggctgtt gtgcggcagg
     3421 ttgtgtttct tggaaacata ttcctgagaa tcacgcagga tggtcggagt cagaaccacg
     3481 ttacccggct tgtaaacacc gtgtacgtta ccgaaggacg cagcgatggt gaaacgcggg
     3541 ctgattttgc tcagttcggt gtatgcgtaa tcaacgtctt ccggctgggt gtacagtgca
     3601 gaagcgtcca tgtggctgtt gtccacgccg tcttcttcac caccggtgca acccagttcg
     3661 atttccagag tcatgccgat tttggacatg cgctccaggt atttagagca gatttcgatg
     3721 ttctcttgca gagattcttc agacaggtcg atcatgtgag aagagaacag cggcttaccg
     3781 gtagctgcga agtgtttttc acccgcgtcc agcagaccgt cgatccacgg cagcagtttc
     3841 ttcgcgcagt ggtcagtgtg caggataacc ggaacaccat aatgttcagc catctggtga
     3901 acgtgatgcg caccagagat cgcgcccagg atagcagcac cctgcggaac gtcagatttc
     3961 acgcctttac cagcgataaa ggaagcacca ccgttggaga actgaacgat aaccggcgct
     4021 ttaactttag cagcggtttc cagtacggcg ttgatggagt cagtaccgac gcagtttact
     4081 gctggcagtg cgaagttgtt ttcttttgct acctggaaaa ctttctgtac gtcatcacca
     4141 gtgattacgc caggttttac gaaatcaaaa atcttagaca tgtctcttgt cctgtatcgt
     4201 cgggccttat actcatcata cttcaagttg catgtgctgc gtctgcgttc gctcacccca
     4261 gtcacttact tatgtaagct cctggggatt cactctcttg tcgccttcct gcaactcgaa
     4321 ttatttagag tatgaaaaaa tgcgtgctct aaaagcgcgc tgaaacaagg gcaggtttcc
     4381 ctgccctgtg attttttact tcttagcgcg ctcttcgagc atcgctactg caggcagtac
     4441 tttaccttcc acgaattcga ggaatgcgcc gccgccagtg gagatgtagg agattttgtc
     4501 agcaatgccg aacaggtcga ttgctgccag agtgtcgccg ccgccagcga tggagaacgc
     4561 ttcgctgtct gcgatagcgt tagccacgat ttcagtacct ttgcggaagt tcgggaattc
     4621 gaacacgcca accggaccgt tccacagaat ggttttcgca ttcttcagga tttcagccag
     4681 ttcctgagcg gaagcatcac cgatatccag gatctgctcg tcagctttca catcgttaac
     4741 agatttcagg gtagccgatg cagtttcaga gaactcggtt gctacgcgaa catcagacgg
     4801 aaccgggatg ttgcaggtgg tcagcagacg tttagcttcg tcaaccaggt cagcttcgta
     4861 cagggattta cccacatcgt ggccttgtgc cgcgataaag gtgttagcga taccaccacc
     4921 aacaatcagc tggtcagcga ttttagacag ggagtccaga acggtcagtt tggtagatac
     4981 tttagaacca ccaacgatag ccaccatcgg gcgagcaggt tctttcagtg ctttacccag
     5041 cgcgtccagt tcagctgcca acagcgggcc tgcgcacgca acatcagcga atttaccgat
     5101 accgtgagta gaagcctgcg cgcggtgagc agtaccgaat gcgtccatta cgaacacgtc
     5161 acacagtgca gcgtattttt tggacagggt ttcgtcgtct ttcttctcgc ctttgttgaa
     5221 gcgaacgttt tccagaacaa ccagttcacc ttcagcaacg tcaacgccgt cgaggtaatc
     5281 tttaaccaga cgaaccgggt tagacagttt gtctttcagg tagttaacaa ccggcagcag
     5341 agagaattct tcgttgtact cgccttcggt aggacgaccc aggtgggaag ttaccatcac
     5401 tttcgcgcct tgtttcaggg ccagttcgat ggtcggcaga gaagcacgga tacgcgcgtc
     5461 gctggttact ttcccgtctt ttactggtac gttcagatcc gcacggataa atacacgttt
     5521 cccagcaaga tccagatcgg tcatcttaat tacagacatg gtgaatcctc tcgttgattc
     5581 taaaagtttt gcagacgctg cttgcgtctt acctgaaagc aacagtagcc atagctaacg
     5641 tcgtgtcgag cattcggtta gcaaagcccc attcgttatc gcaccagacc aacgttttga
     5701 tcaggtgtgc gccactgacc cgggtttggg tgccatcgac aatggcactg tgcggatcgt
     5761 ggttaaaatc tacagagacc aacggcaatt ccgtatagtc aactatacca tgaaatgcac
     5821 cttgtgctgc tttttgcagc aacaggttga cttcattggc ttttacaggt ttcttcaccg
     5881 tcacgcttaa atcgattgcc gtcacattta tggttggcac acgtaccgca atcgcttcaa
     5941 agcgatcgtt aaattgcgga aaaaatcgtg tgataccggc ggccagttta gtatcgaccg
     6001 gaatgatcga ctggctggct gcccgggtgc gacgcaggtc aggatggtat gcatcaataa
     6061 cctgttgatc gtgcatggcg gagtgaattg tggtcacagt gccggactca ataccgtacg
     6121 catcatctaa caatttgatg acgggaatta tgcaattcgt ggtacaggaa gcgttagaaa
     6181 cgatgcggtg ttccgcacga agttgatcct gattgacgcc gtaaacaacg gtcgcgtcga
     6241 gatcgttact gccaggatgt gaaaagagca cttttttggc cccggcggca atatgcgctt
     6301 cgccatgctc gcgggagcca tatacgccgg tgcagtcgag gactacatca acgccaagtt
     6361 cacgccaggg gagcgattgc agtgaacgtt catgcaatac gcggatggcg tcatcaccaa
     6421 caaaaagttg atcgcgttcc tgtcgtactt cccatgcaaa acggccatgg ctggtgtcat
     6481 atttcaacaa atgcgccatg cccgcagcat ccgccagttc gttgattgcc accacggtaa
     6541 tttccgcccg gcgtccggat tcatacaaag cacgaaccac attacgcccg atgcgaccga
     6601 agccatttat cgctacgcgt acggtcatag atctcctgca aggttttccc tgagcaaatt
     6661 tgccagacag agtaatccag caaatcgtcc ggggaaacct tacctgtcgc aaactgcgac
     6721 tgattggtta attgtcgaac atttaatcga ctgaaacgct tcagctagga taagcgaaac
     6781 gtggaataaa aggaatgatt gtccagtcaa agaaaacaat tatctgactt acgtcacgtt
     6841 ttcagccccg cccctgtgga atttattcca ctttgcagaa tcacgaataa gcgtagcaaa
     6901 atcttttcgt tgtgccgtca caaaccctga cttatatcag aatatgactg atgcagcgtc
     6961 gatattatcc gcaatacgtc cgagtttcgg agtactccaa tgatgacatt gccctaccca
     7021 tattcttccc ccgcacgctt ttatgcactg cgcctcctcc ccgggcagga agttttttcc
     7081 cagctacacg cttttgcaca gcagcatcaa cttcatgccg cctggattgc aggctgtacc
     7141 ggtagcctga ctgacgtcgc cttgcgctat gccgggcaag aaggcaccac gctccttaac
     7201 ggtacattcg aagtgatctc actaaacggc acgctggaac agagtggtga gcatcttcat
     7261 ctctgcgttt ccgatccaca cggcacgatg cttggcggtc atatgatgcc cggctgtacc
     7321 gtgcggacca cgcttgaact ggtgatcggt agccttgagg aactggcgtt tagccgccaa
     7381 ccttgcgcgc tttctggtta cgacgagttg catatttccc ccgttaaata actactttca
     7441 aagaggtttt cttatggcgc gtagccactt ttccagtcag gcactggtgt tgatcgttat
     7501 atctatcgcc atcaatatga tcggcgggca actggccagc atggtcaaat tacctatatt
     7561 tctcgactcc atcggcacac ttattagcgc ggtactgctt ggtccggtga tcggaatgct
     7621 gaccggctta ctgaccaatt tactctgggg attgctgacc gacccgatcg ccgccgcatt
     7681 tgcacccgtc gcgatggtca tcggcctggt cgccggttgg ctggcgcgag cgggttggtt
     7741 tcgcaccctg ccaaaagtcg ttgtcagcgg cgtgattatt acgcttgcgg taacggtcgt
     7801 cgctgtaccg ctgcgtactg cgctgttcgg cggtgtcacc ggtagcggag ccgatttgtt
     7861 cgttgcctgg atgcactcga tggggcaaaa cctggtggag tcggtggcga ttacggtgat
     7921 aggcgcgaac ctggtcgata aaattcttac agcggtgatt gtctggcttt tactgcgcca
     7981 gctgccgatt cgcaccacgc gccattttcc ggcaatggct gccgtgcgct aaatgcatcc
     8041 atttacctca ttaacactgt gggcattggc ggcctgcacc acgcttatcc tgcccgcgca
     8101 gaccattttg ccgatctata gcgcggcgac ctttttttgt cttattgcgc taaaggccac
     8161 gcggcggcgg gcaaaatatg tcgcctggct gatgttctcg ctgggagccg ggctgtggct
     8221 ggtacacggt ggctggctaa cagaatggct aagcggcacg ccgcgttcgc cggagcgttg
     8281 gacgcatgct attacgctct ggttgcggat tctggcgatt gtttcaacct cacagttgtg
     8341 gatgcagtac gtccccgtgc aatgttttat ccgcgctctg tttgcttctc gcctgccgcc
     8401 aggcgtcgct tatctgtttg ccggaccgtt attggtcgtt gagcagttaa aacggcaatt
     8461 agcgattatt catgaagcgc agcgtgcccg cggcgtgccg ctggatgagg gctggtatca
     8521 gcgtttacgt gcgatgcccg cgttaattat tctgctaaca cacaatgcgc ttaacgatct
     8581 ggctgtccgt ggtgcggcgc tggatatgcg ggcatttcgg atccacaacc gacgaacaac
     8641 gttgtgggca ccagccgata gcaccctaca acgcgtagcg cgttacacca tgatcttgct
     8701 gatgctaaca gaattcggag cgtggatatg gttacgttag aacagttccg ttattgcccg
     8761 acacactcaa cgcatcctcc gttctgttat gactttcact atgtcaaacc ggggatggtc
     8821 gccatctttg gtgacaacgg cagtggcaaa agtacgctgg cacagctgat ggcgggctgg
     8881 tatccggact ttctgccagg agagataaca ggcacgggga cgctcctcgg tacgcccatt
     8941 gggcatctcc cgcttaatga acaatccgcc accatccagc tcgtccagca atcgccctat
     9001 ttgcagctct ccggctgtac ctttagcgtg gaagaagaag ttgcctttgg gccagaaaat
     9061 ctgtgtcttg ctgaaacaga gattatggcg cggattgatg ccgccctgac tctgaccgag
     9121 tgccagccgt tgcgccatcg tcacccggca acactctccg gtggagagac acaacgcgtg
     9181 gtcatcgcct gcgccatcgc gatgcagcca aagctcttaa ttcttgatga agcctttagt
     9241 cgcctgacgc cgcaggccag cgaaatgctg ttgcagcgct tacaacactg ggcgcttgaa
     9301 cgtggttcgc tgattattct ttttgaacgt catcgcacac ctttcctgaa ctactgccag
     9361 caagcgtggc aattgcaaaa cggagcgctc cagccactat gttaacgtta aatcagatat
     9421 cttatcgctg gcctagcgca gcaacggatt gtctttgcga catttctctg caactcaaac
     9481 aaggtgaatg gttggcgctg accggcgaca atggcgcggg aaaatcgacg ctgttacgag
     9541 taatggcagg tcttcttacg cccacagccg gtacggttat gctgcaacaa caggcgatga
     9601 aaaacctgaa aaaccgccag cgcgcggcaa aggttggcgt actgtttcag gaagcggaaa
     9661 accaactttt tcacagcacc gtcgctgacg agatcgcatt tggtttgaaa ctgcaaaaat
     9721 gcccggcgga tgaaatcact caacgtaccc atgctgccct gcaatgttgt cagttagcag
     9781 acactgccaa ctcgcacccg ttggatctgc atagtgcgca gcgacggatg gtcgccgtcg
     9841 cctgcctgga agcactctct ccgccactat tactgctgga cgagccgagt cgggattttg
     9901 acgaaaattg gttgagcgtg tttgaaagct ggctggagaa gtgccgccag cgaggcacga
     9961 gcgtggtagc aatcagtcat gacgccgcat ttacccggcg tcatttctct cgtgtggtgc
    10021 gactggaaga tggtgtaatc aggaacgtta atccaccaga cgatattcac ccgtagcggt
    10081 catcattaag gtcaaatttg caggcagact ctcttccagc acccggcgaa cattcggccc
    10141 gtcggtacgg tcataaaagg cttctgcatc tgccagcgat acccctcccg ccagcttgcg
    10201 ccccaccaga cgctcccgca acgcagtagc tggcgcgttg ataaagatcg agaaatcaca
    10261 aaattgcgca agctggcacc acttctcgtc gtccagcaac aaccagtttc cttcgacgat
    10321 gacgagtggt gcggtaacgt gcaacgcatc ttcaacagga tcatgctttt gtcgatcgta
    10381 ctgcggccac gtacaatccc cttccaccac ctggcgcaga ttttccgcca gtttcgcaac
    10441 gtcaaatgtc tctggtgcgc ctttgaaggg gcgcaattga tgcgcgtcca gccagctatt
    10501 gtaatggtga aaaccatcca tcgggagcgt ctgaatagcg ggcagctccg ggtcttgttg
    10561 cacgagatat tcccagaagg tggttagggt agatttaccc gtccccggcg gagcgcaaag
    10621 aaaaaccacc gtacgccgct gcggatttac cgtctgtaag gccgccagca tacgcagcaa
    10681 cggtttatgc acgcgttcta tctcttcatc atggtactgc gcttgcacgt tcagcccatt
    10741 caccgttaat tcaattttca cggcgttaat tcctttaagg tggtcgttac tgccagcgtc
    10801 agcgccgttt tgaccattcc gctaaaacgc gcc
//
LOCUS       DAESHI010000138        10773 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.140, whole genome shotgun sequence.
ACCESSION   DAESHI010000138 DAESHI010000000
VERSION     DAESHI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10773
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.140"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..656
                     /locus_tag="KW011_003673"
     CDS             <1..656
                     /locus_tag="KW011_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH7661917.1"
                     /translation="VMRTHFPEYEIISSASAEDLTLLQLRRSGLVIADLAGESEDPRS
                     VCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGN
                     THAERISAMLTSPAMNETHDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKSNKT
                     ISAQKNSAMRRLAIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSHS"
     gene            614..1291
                     /gene="bglJ"
                     /locus_tag="KW011_003674"
     CDS             614..1291
                     /gene="bglJ"
                     /locus_tag="KW011_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HBH7661918.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1329..2117)
                     /gene="fhuF"
                     /locus_tag="KW011_003675"
     CDS             complement(1329..2117)
                     /gene="fhuF"
                     /locus_tag="KW011_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HBH7661919.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2258..2494
                     /locus_tag="KW011_003676"
     CDS             2258..2494
                     /locus_tag="KW011_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HBH7661920.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            complement(2533..2619)
                     /locus_tag="KW011_003677"
     tRNA            complement(2533..2619)
                     /locus_tag="KW011_003677"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2583..2585),aa:Leu,seq:cag)
     gene            complement(2654..2740)
                     /locus_tag="KW011_003678"
     tRNA            complement(2654..2740)
                     /locus_tag="KW011_003678"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2704..2706),aa:Leu,seq:cag)
     gene            complement(2769..2855)
                     /locus_tag="KW011_003679"
     tRNA            complement(2769..2855)
                     /locus_tag="KW011_003679"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2819..2821),aa:Leu,seq:cag)
     gene            complement(3123..4154)
                     /gene="rsmC"
                     /locus_tag="KW011_003680"
     CDS             complement(3123..4154)
                     /gene="rsmC"
                     /locus_tag="KW011_003680"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HBH7661921.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4257..4670
                     /gene="holD"
                     /locus_tag="KW011_003681"
     CDS             4257..4670
                     /gene="holD"
                     /locus_tag="KW011_003681"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HBH7661922.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            4639..5085
                     /gene="rimI"
                     /locus_tag="KW011_003682"
     CDS             4639..5085
                     /gene="rimI"
                     /locus_tag="KW011_003682"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBH7661923.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            5100..5777
                     /gene="yjjG"
                     /locus_tag="KW011_003683"
     CDS             5100..5777
                     /gene="yjjG"
                     /locus_tag="KW011_003683"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HBH7661924.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            5868..7457
                     /gene="prfC"
                     /locus_tag="KW011_003684"
     CDS             5868..7457
                     /gene="prfC"
                     /locus_tag="KW011_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBH7661925.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            7850..8455
                     /gene="osmY"
                     /locus_tag="KW011_003685"
     CDS             7850..8455
                     /gene="osmY"
                     /locus_tag="KW011_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HBH7661926.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            8582..8743
                     /locus_tag="KW011_003686"
     CDS             8582..8743
                     /locus_tag="KW011_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HBH7661927.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            8865..9938
                     /locus_tag="KW011_003687"
     CDS             8865..9938
                     /locus_tag="KW011_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HBH7661928.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            9935..10717
                     /locus_tag="KW011_003688"
     CDS             9935..10717
                     /locus_tag="KW011_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBH7661929.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
BASE COUNT         2619 a         2700 c         2876 g         2578 t
ORIGIN      
        1 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
       61 ttacattatt acaattacgt cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      121 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      181 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaattg ctcatgtgcc
      241 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      301 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctctccggca atgaatgaaa
      361 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      421 tattaggtaa aggatggggc atcaaccaga tagcttcgtt gcttaagaaa agtaataaaa
      481 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc aattcacagc aacgctgaaa
      541 tgtatgcatg gataaatagc gcgcagggtg caagagaact taacttgcct tctgtttatg
      601 gagatgccgc agaatggaac acagccgaat taagaagaga aatgtcgcac tcatagaaaa
      661 atgcgtcatg agcagtatcg gtattgagag tttattcaga aagtttgcgg gtaaccctta
      721 taagctccat acctatacca gtcaggagtc atttcaggat gccatgtcgc ggatctcatt
      781 tgcggcggtc attttttctt tttctgccat gagaagtgag cgcagagagg gattatcttg
      841 cctgactgaa ctggcgatta agtttccgcg tacccggcgt ttagttattg cggatgatga
      901 tattgaagct cggctgattg gttcattgtc gccatcaccg ctggacggtg tattaagtaa
      961 agcgtcaacg ctggagattt ttcatcagga actctttttg tcattaaatg gtgtacgtca
     1021 ggcgaccgac cgactgaaca atcagtggta cattaaccaa agccggacgc taagcccgac
     1081 ggagagagaa atattgcgct ttatgtcgcg tggctactca atgacacaaa ttgccgagca
     1141 gcttaaacgc aatatcaaaa cgatccgtgc acataaattt aatgtgatgt cgaaactggg
     1201 cgtcagttct gatgcagggt tgttggaggc cgcagatatt ctgttatgta tgcggcattg
     1261 cgaagcaagt aatgtgttgc atccctatta atccgcatga tgccgggttt acttccccgg
     1321 cagtgctttc atttcagcgt acaatcgcca cattgctgca catccggtaa gcgataacgc
     1381 tggcagcagg ttcggcgcac cagcaggcca tcgcgcagca cgacggtacg ccagagtgga
     1441 ttatcttcac cgttcgtgag cgttttctca aaaaagaggg catggcgcag cgattcaacc
     1501 gtcgcctcgc cgagcagttg cttcatctca gtgagatacc agttgatcaa ataaccggta
     1561 ttactccaga taagtttgcc gttgatctct ccggtcgctt ctagtgcctg cacaaccgga
     1621 accagcgcct ggctgattaa cgtttccatt cgctgctgcg gcgaatgtgg tgttgcgttt
     1681 ttatcttcac acacatcgac ccagaaacag gcgacgcgtc cggtttcgtg aaactcagca
     1741 tggaaatgtt ccggcgacac atctaatgcc ttttcctgcg tcagtagcgc cagcattaat
     1801 ggtggcacca tcaggccgat atacaattgt gcccatagtg agatcagcgg tttgttctcg
     1861 cggatcatcg ttggttggtt gcgatagata tgatcggaat agaccgcgag tagagaactt
     1921 agcgcattcg gtgatgacca ttgcgccagc gtcatggcgt taagcggagc gggttcatcc
     1981 aggcggataa actcgagcaa atgttcacga tgtttggcga tcgtcgcacg tacggcttgt
     2041 gcaagcccag catcctgcgg ctggagatgc gttcgccaga tgatatcttc atagagcggt
     2101 gcggaacgat aggccataat cgggatagta atctaaatga taatgattgc taatcctagc
     2161 gataggttta cccgatagca agggatttct ctggcttgca aatgataaaa attatcatat
     2221 gatattggtt atcattatca atgaaagaga tgaaaccatg ttgcaacgta cgctgggcag
     2281 tggctgggga gtgttgctgc cgggattgct gattgcaggg ctgatgtatg cggatttatc
     2341 gccggatcag tggcggattg tcattctgat gggattagta ttgacgccgg tgatgctgta
     2401 tcacaaacag ttacggcatt acgttttgct accatcgtgc ctggcgctta ttgctgccat
     2461 catgctgatg ataatgaatt tgaatcaggg atgaaaaatc aaggaagaaa caagaaagga
     2521 agtaaagata attggtgcga ggggggggac ttgaaccccc acgtccgtaa ggacactaac
     2581 acctgaagct agcgcgtcta ccaattccgc caccttcgca cagtcatctt actttttttg
     2641 atatcgcctc gtttggtgcg aggggggaga cttgaacccc cacgtccgta agaacactaa
     2701 cacctgaagc tagcgcgtct accaattccg ccaccttcgc ccagtgcgag caatatcaac
     2761 gtggtttttg gtgcgagggg ggggacttga acccccacgt ccgtaaggac actaacacct
     2821 gaagctagcg cgtctaccaa ttccgccacc ttcgcatacc atcaattctt aaaaagaatt
     2881 gctaccacgg aggcgcattc tagtggtttt cagcttttcg tcaatagtta attatcgccg
     2941 gaggtgtaat tgctggaaaa atgtccatca ggaaactagc gtgcaggttt ggtaggcatg
     3001 cgggggcaga tgccagatgc gacgctggcg cgtcttatct ggcctacgaa gggctaacgt
     3061 gcaggttttg taggtcggat aaggcgttca cgccgcatcc gacacggtat tcggcgagat
     3121 aattaacctt tcttcgcctg gcgggtcata atggcgcgat acaccttgaa acgcccggtt
     3181 tgcgcaatca cttcatggaa gccaaatgtc tcatccagca cgtccgggta aggcaggaag
     3241 gcgttcgcta caattcgcag ctcgccgccg ctattaagat gacgcaccgc gccgcgaatc
     3301 agcgtttgcg ccgcatccag gctggtttgc atcccatcgt ggaacggcgg gttggagatg
     3361 atcatatcaa aacgaccttt cacctcggaa aagacgttgc tggcaaagac ttcaccttca
     3421 acacagttgg ccgcaagtgt tgcgcggctg gcttctaccg ctggcgcaga gacatcgcac
     3481 aaggtgagac gaattttcgg cgaatggcgc gcaaaggcaa ctgaaagcac ccccgcgcca
     3541 cagccgacat ccagcacttt acctttcgtg tgcggagtta acgtcgagag cagcaactgg
     3601 ctaccgacat ccagaccgtc gcggctaaac acgccaggca gcgttttgac cgtcaggcca
     3661 tcgacgctgt attcgcccca gaatttatcg gcatcaaata ccggctgttt ttccagacga
     3721 ccaaaataga ggccacagcg acgagcgctg tcgactttat tcaacggcgc ataatctgcc
     3781 agcatctgct cggcgctgcg cacgccgctg cggttctcgc caacgacaaa aatatctgtc
     3841 cccactggca gcagagaaag taaattcatc aactggaact gggcttccgg tttgttcttc
     3901 ggccagtagt aaatcagcgt atcgcaatct gcgacgtcat ccaccgtggc gaccagactg
     3961 aaacgggcgt tatcccccat ctggcggctt aatacctgcc agtggtggaa ttgctgggta
     4021 tgagcacggc tggccgcggt atctaaacgc gcgggcaggt catcctgtaa gtctccggca
     4081 aacagaatac ggctttgttc gaaatcatca ctgtgacgca gcaagacttc acttgccggg
     4141 gtaaatgcag acatggaatg ctcctcaatt gatactggcg gcgattatag ccatatgttg
     4201 gcgcggtatc gacgaatttg ctatatttgc gcccctgaca acaggagcga ttcgctatga
     4261 catcccgacg agactggcag ttacagcaac tgggcattac ccagtggtcg ctgcgtcgcc
     4321 ctggcgcgtt gcagggggag attgccattg cgatcccggc acacgtccgt ctggtgatgg
     4381 tggcaaacga tcttcccgcc ctgaccgatc ctttagtgag cgatgttctg cgcgcattaa
     4441 ccgtcagccc cgatcaagtg ctgcaactga cgccagaaaa aatcgcgatg ctgccgcaag
     4501 gcagtcgctg caacagctgg cggttgggta ctgacgaacc gctatcactg gaaggcgctc
     4561 aggtggcatc accggcgctc accgaattac gggcaaaccc aacggcacgc gccgcgttat
     4621 ggcaacagat ttgcacatat gaacacgatt tcttccctcg aaacgactga tttaccagcg
     4681 gcttaccaca ttgaacaacg cgcccacgcc tttccgtgga gtgaaaaaac ctttgccagc
     4741 aaccagggcg agcgttatct caactttcag ttaacgcaaa acggcaaaat ggcggcgttt
     4801 gcgattacgc aagtggtgct ggatgaagct acattgttca atattgcggt cgatcctgac
     4861 tatcagcgtc agggattggg aagggtgctg ctggaacatc tgatcgacga actggaaaaa
     4921 cgcggcgtgg cgacactatg gctggaagtc cgtgcctcaa atgctgccgc cattgccctg
     4981 tacgaaagtt taggctttaa cgaggcgacg attcgccgca attactaccc caccacggac
     5041 ggtcgcgaag acgccatcat catggcgttg ccaatcagta tgtaagacaa ggtggaataa
     5101 tgaagtggga ctggattttc tttgatgccg atgaaacgct gtttaccttt gactcattca
     5161 ccggcctgca gcggatgttt cttgattaca gcgtcacctt taccgctgaa gattttcagg
     5221 actatcaggc cgttaacaag ccactgtggg tggattatca aaacggcgcg atcacttcat
     5281 tacagcttca gcacgggcgt tttgagagct gggccgaacg gctgaaagtt gaagcaggct
     5341 tgcttaacga tgcctttatt aatgcgatgg cggaaatctg cacgccgctg ccgggcgcgg
     5401 tttctctgct taacgccatt cgtggcaacg ccaaaatcgg catcatcacc aacggcttta
     5461 gcgccttgca gcaagtgcgt ctggaacgca cgggcctgcg tgattacttc gatttgctgg
     5521 tgatttccga agaagttggc gttgccaaac cgaataagaa aattttcgat tatgcgctgg
     5581 aacaggcggg caatcctgac cgttcacgcg tgctgatggt tggcgacact gccgagtccg
     5641 atattctcgg tggcatcaac gccgggcttg cgacctgctg gctgaatgcg cacaatcgcg
     5701 agcaaccaga aggcatcgcg cccacctgga ccgtttcttc gttgcacgaa ctggagcagc
     5761 tcctgtgtaa acactgattg cctccccccc gttgatgggt aaaatagccg caatttttcg
     5821 ttttcaacaa gcgcggcgcg atgccgctta ctcaagaaga aagaattatg acgttgtctc
     5881 cttatttgca agaggtggcg aagcgccgca cttttgccat tatttctcac ccggatgccg
     5941 gtaagactac catcaccgag aaggtgctgc tgttcggaca ggccattcag accgccggta
     6001 cagtaaaagg ccgtggttcc aaccagcacg ctaagtcgga ctggatggag atggaaaagc
     6061 agcgtgggat ctccattact acgtctgtga tgcagtttcc gtatcacgat tgcctggtta
     6121 acctgctcga caccccgggg cacgaagact tctcggaaga tacctatcgt accctgacgg
     6181 cggtggactg ctgcctgatg gttatcgacg ctgcaaaagg tgttgaagat cgtacccgta
     6241 agctgatgga agttacccgt ctgcgcgaca cgccgatcct cacctttatg aacaaacttg
     6301 accgtgatat ccgcgacccg atggaactgc tcgatgaagt tgagaacgag ctgaaaatcg
     6361 gctgcgcacc gatcacctgg ccgattggct gcggcaagct gtttaaaggc gtttaccacc
     6421 tttataaaga cgaaacctat ctctatcaga gcggtaaagg ccacacgatt caggaagtcc
     6481 gtattgttaa agggctgaat aacccggatc tcgacgctgc ggttggtgaa gatctggcac
     6541 agcagctacg tgacgaactg gaactggtga aaggcgcgtc taacgagttc gacaaagaat
     6601 tgttccttgc gggcgaaatc actccggtat tcttcggtac tgcgctgggt aacttcggcg
     6661 ttgatcatat gctggatggc ctggtggagt gggctcctgc gccgatgccg cgtcagactg
     6721 atacccgtac cgtagaggcg agcgaagaca aatttaccgg cttcgtattt aaaattcagg
     6781 ccaacatgga cccgaaacac cgcgaccgcg tggcgtttat gcgcgtagta tccggtaaat
     6841 atgaaaaagg catgaagctg cgccaggtgc gtactgcgaa agatgtggtt atctccgacg
     6901 cgctgacctt tatggcgggc gaccgttcgc acgttgaaga agcgtatccg ggcgatatcc
     6961 tcggcctgca caaccacggc actattcaga tcggcgacac ctttacccag ggtgagatga
     7021 tgaagttcac cggtattccg aacttcgcac cagaactgtt ccgtcgtatc cgcctgaaag
     7081 atccgctgaa gcaaaaacag ctgctcaaag ggctggtaca gctttccgaa gagggcgcgg
     7141 tgcaggtgtt ccgtccgatc tccaacaacg atttgatcgt tggtgctgtt ggtgtgctgc
     7201 agtttgatgt ggtggtatcg cgcctgaaga gcgaatacag cgttgaagca gtatatgaat
     7261 cagtcaacgt tgccactgcc cgctgggtag aatgtgcgga cgcgaagaaa ttcgaagagt
     7321 tcaagcgtaa gaacgaaagc caactggcgc ttgatggcgg cgataacctc gcttacatcg
     7381 ctaccagcat ggtcaacctg cgcctggcgc aggaacgtta tcccgacgtt cagttccacc
     7441 agacccgcga gcattaattc ttgcctccag ggcgcggtag ccgctgcgcc ctgtcaattt
     7501 cccttcctta ttagccgctt acggaatgtt cttaaaacat tcacttttgc ttatgttttc
     7561 gctgatatcc cgagcggttt caaaattgtg atctatattt aacaaagtaa tgacatttct
     7621 gacggcgtta aataccgttc aatgcgtaga tatcagtatc taaagccgtc gattgtcatt
     7681 ctaccgatat taataactga ttcagaggct gtaatggtcg ttattcatca cccatcgctt
     7741 ttgtgatggc gaccattgac ttctgtagag ggtgaagtct ctccctattc agcaatgcaa
     7801 cctcgtgttg ccaggctcaa attacgagca aacatacagg aataaatcga tgactatgac
     7861 aagactgaag atttcgaaaa ctctgctggc tgtaatgttg acctctgccg tcgcgaccgg
     7921 ctctgcctac gcggaaaaca acgcacagac taccaatgaa agcgcagggc aaaaagtcga
     7981 tagctctatg aataaagtcg gtaatttcat ggatgacagc gccatcaccg cgaaagtgaa
     8041 ggcggcgctg gtggatcatg acaacatcaa gagcaccgat atctctgtca aaaccgatca
     8101 aaaagtcgtg accctgagcg gtttcgttga aagccaggct caggccgaag aggcagtgaa
     8161 agtggcgaaa ggcgttgaag gggtgacctc tgtcagcgac aaactgcacg ttcgcgacgc
     8221 taaagaaggc tctgtgaagg gctacgcggg tgacaccgcc accaccagtg aaatcaaagc
     8281 caaactgctg gcggacgata tcgtcccttc ccgtcatgtg aaagttgaaa ccaccgacgg
     8341 cgtggttcag ctctccggta ccgtcgattc ccaggcacag agcgaccgtg ctgaaagtat
     8401 cgccaaagcg gtagatggtg tgaaaagcgt taaaaatgat ctgaaaacta agtaattcgt
     8461 cgtaattcgt cctcccgaac tttgtcggga ggcgcgatgt gcaccacact aaaaatatcg
     8521 cattgagtga ttttcacgct catatcaagc ggtgacatta actatggtaa aggagacgct
     8581 tatgtttcgt tggggcatca tatttctggt tatcgcgtta atcgccgccg cacttgggtt
     8641 tggtggtctg gccggtaccg ctgcaggcgc agctaaaatt gtctttgtcg tcgggattat
     8701 tctgttcctg gtgagtttgt tcatgggccg aaaacgaccc tagatttcag accattaaga
     8761 aaattttatc caaagccagt ccagcggact ggcttttgcg gttttagcga ataataaatt
     8821 gcgttacttt gtcattcttt cacaacaaaa acaggaaggc agaggtgggg cagcgaatac
     8881 ctgtaacgct tggtaatatt gcgccgttgt cgctaaggcc gttccagcct ggacgaatag
     8941 ctctggtgtg cgaaggcggc ggacagcgtg gaattttcac ggctggcgtg ctggatgagt
     9001 ttatgcgcgc gcagtttaat cctttcgatc tttatctcgg cacatctgcc ggggcgcaga
     9061 acctctcggc gtttatctgc aatcagcccg gttacgcgcg caaagtcatc atgcgctata
     9121 ccacaaaacg cgaatttttc gatccattgc gctttgtccg tggaggaaat cttatcgatc
     9181 tcgactggct ggtggaggcc accgcaagcc agatgccgtt gcaaatggat accgccgcgc
     9241 ggttgtttga cagcggcaaa tcgttttata tgtgcgcctg tcgtcaggat gactacgcgc
     9301 ctaattactt tttaccaacc aaacaaaact ggctggatgt gattcgcgcc tccagtgcga
     9361 tacctggctt ttatcgtagc ggagtgtcgc tggaaggcat taactacctg gatggcggga
     9421 tcagtgatgc gattccggtt aaagaggcgg caaggcaggg cgctaaaacg ttggtcgtca
     9481 ttcgcactgt gccgtcacaa atgtactaca cgccgcagtg gttcaaacgc atggaacgct
     9541 ggctgggtga cagtagcctg cagccgctgg tcaatctggt gcagcatcat gaaaccagct
     9601 atcgtgacat tcagcaattt attgagaaac caccgggcaa gctgcggata ttcgaaattt
     9661 atccgccgaa gccattacat agtatcgcgc ttggcagtcg gattccggcg ctgcgtgaag
     9721 actataaact tgggcgttta tgcggtcgtt atttcctcgc cacggttggc aagctattaa
     9781 ctgaaaaagc gccgcttacc cgccatctgg tgccagtggt gacgccggaa tcgatcgtta
     9841 ttccgcctgc gccagtcgcc aacgatacgc tggttgccga agtgagcgat gctccgcagg
     9901 cgaacgaccc gacatttaac aatgaggatc tggcttgatt tgccgtttta tcgataccca
     9961 ctgccatttt gatttcccgc cgtttagtgg cgatgaagag gccagcctgc aacgcgcggc
    10021 acaagcgggc gtaggcaaga tcattgttcc ggcgacagag gcggaaaatt ttgcccgcgt
    10081 gcaggcgtta gcggaaaaat atcaaccgct gtatgccgca ttggggttgc atccggggat
    10141 gttggaaaaa catagcgatg tgtctcttga tcagctacag caggcgctgg aaaggcgtcc
    10201 ggcgaaggtg gtggcggtgg gggagatcgg tctggatctc ttcggcgacg atccgcaatt
    10261 tgagaggcag cagtggttac tcgacgaaca gctgaaactg gcgaaacgct acgatctgcc
    10321 ggtgatcctt cattcccggc gtatacacga caaactggcg atgcatctta aacgtcacga
    10381 tttaccgtgc actggcgtgg ttcacggttt ttccggcagc ctgcaacagg ccgagcggtt
    10441 tgtacagctg ggctacaaaa tcggcgtagg cggtactatc acctatccac gcgccagtaa
    10501 aacccgcgat gtcatcgcaa aattaccgct ggcatcgttg ttgctggaaa ccgacgcgcc
    10561 ggatatgccg ctcaacggct ttcaggggca gcctaaccga ccggaacagg ctgtgcgggt
    10621 gtttgacgtg ctttgcgagt tgcgcccgga accagaagat gagattgccg aagtgttgct
    10681 taataatacg tatgcgttgt ttagcgttag tgggtagcct acccttcgta ggcagcatcc
    10741 ggcagtcggc gcataatgcc tgatgcgacg ctt
//
LOCUS       DAESHI010000139        10685 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.141, whole genome shotgun sequence.
ACCESSION   DAESHI010000139 DAESHI010000000
VERSION     DAESHI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10685
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.141"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..784)
                     /locus_tag="KW011_003689"
     CDS             complement(<1..784)
                     /locus_tag="KW011_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661930.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            957..1835
                     /gene="yihU"
                     /locus_tag="KW011_003690"
     CDS             957..1835
                     /gene="yihU"
                     /locus_tag="KW011_003690"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HBH7661931.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1860..2747
                     /locus_tag="KW011_003691"
     CDS             1860..2747
                     /locus_tag="KW011_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBH7661932.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASVVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2780..3790
                     /locus_tag="KW011_003692"
     CDS             2780..3790
                     /locus_tag="KW011_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBH7661933.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDVVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3860..5293
                     /locus_tag="KW011_003693"
     CDS             3860..5293
                     /locus_tag="KW011_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH7661934.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5392..6741
                     /locus_tag="KW011_003694"
     CDS             5392..6741
                     /locus_tag="KW011_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HBH7661935.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6731..7618
                     /locus_tag="KW011_003695"
     CDS             6731..7618
                     /locus_tag="KW011_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HBH7661936.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7758..8357
                     /gene="yihX"
                     /locus_tag="KW011_003696"
     CDS             7758..8357
                     /gene="yihX"
                     /locus_tag="KW011_003696"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HBH7661937.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8351..9223
                     /locus_tag="KW011_003697"
     CDS             8351..9223
                     /locus_tag="KW011_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HBH7661938.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9220..9657
                     /gene="dtd"
                     /locus_tag="KW011_003698"
     CDS             9220..9657
                     /gene="dtd"
                     /locus_tag="KW011_003698"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBH7661939.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9702..10643
                     /gene="fabY"
                     /locus_tag="KW011_003699"
                     /gene_synonym="yiiD"
     CDS             9702..10643
                     /gene="fabY"
                     /locus_tag="KW011_003699"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HBH7661940.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2461 a         2530 c         2855 g         2839 t
ORIGIN      
        1 cttttgttgg cattttttct gccaacgcta ccgcgagtgc gccgtgaaaa acatcgcctg
       61 ctccagtggt atcaaccaca ttgacagaaa atgcctcctg ttgacacaaa tgaccatctt
      121 caatccacaa cgatccttca gaacccagcg taacatacac tttacctgct gtttgtgtcg
      181 ttgcctggaa caaaccttct tcaggtgact gcaggccagt catgcgtttc agccctggtg
      241 ttgagaacac ggcgtgatcg gcaagcgcga caagtggaga aatatcctgc ggtgtcatat
      301 cggcgtctaa aagcgttgtc acacccgcaa ggcgcgctag cgagaatgct ttttcagtac
      361 cgctatgcca gcgaacatcg gccagaatca gatcataacg agagaaatcg atcgcttcta
      421 accattcggc atcagtaccg agatcaggac ttggataatt aacgataatc cgttcgccat
      481 gctgatcaac gaggatcgct gattgcgacg aacgggcgtt aggatatcgg cggcaaaatg
      541 cggtattgac gccccaaccc tcaagttctg caagtaaggt atttccacaa ctgtcatcgc
      601 cgacgcgacc gatgaaatca acctccacgc caagctttgc gattgcaaca gcggctgttg
      661 ctgccgggcc gccaccaatc tcaagataat gatttgcctg atatttcccg ccgccctccg
      721 gcagagtggg caaagaataa attctgtcct gaactgtgat acccacgcat gcaattctgg
      781 tcatatttgt cttgtcctgt ttaattttgt cgtctttggt caatttaaat gacgcaaatg
      841 atcaaattag tgatgcgtgt cgtgtttttg aacataaatt acaaatatgc tcaaaaacag
      901 acgaaaacga attttcaatt gtcattattt tgtcaatgtc agacaggaga gtattcatgg
      961 caaccattgc atttctcggg ctggggcaga tgggtagccc aatggctaac aatctattgc
     1021 aaaaagggca ttcattgcag gtatttgatg tcaatgcaca ggccgtcgat gcgctagtta
     1081 cgcagggggc cactgcggca caaaccccag cggaagcagc agcaggcgct gaatttatca
     1141 tcactatgct tcccaatggt gacattgttc gacaggttct gttgggggaa aaaggcgtct
     1201 gtgaggctgt ttcatctgac gcgttattga ttgatatgtc gactattcat cccttacaga
     1261 ccgatgcatt aatacgggag ttacaagaaa aaggcatcag tatgatggac gctcccgtgg
     1321 gcagaacgtc cgtgaatgcg attgatggca cattgctgat tttagcgggt ggcactgatg
     1381 agcagattgc acgtgcacgc ccgattctca tgtgcatggg taatgagctg gtcgaggctg
     1441 gcggcccagg aatggggatc cgcgtgaagc taattaacaa ctatatgagc attgcgctta
     1501 atgcgctgtc ctccgaggcc gctgtgttat gtgagtcact gggattaaat cttgatgtcg
     1561 caattaaggt gatgagtggt acggcagcag ggaaaggaca ttttacgacc acctggcctg
     1621 gcaaagtgct gaaaggcgat ctttcacccg ccttcatggt ggatctggcg ttgaaagatc
     1681 tgcgtattgc agttgacgtc gctcgggaga cgggggcacc gctcaatatg gggattgcag
     1741 cagaaagtta ttacgctgca gcaagtcaag acggcaaagg gcggcaggac tggagtgccc
     1801 tgctaaatca ggttcgccag caggctggtc tttaatttta tctattaaca ggagtttcta
     1861 tgtcatccca cttcacccca tttacgctga aagatatctc ccgcccaggt ggtggatttg
     1921 ccatgttagc tgtcgatcag cgtgaggcca tgcgcttgat gtttgcggca gcagggcagc
     1981 caaaacccat cgctgacagt gtactcaccg attttaaagt tgccgcgacc cgaatccttt
     2041 ctccttatgc ttctgtcgtt ctggcggata aacaattttg tcttgagcaa atagtcgagc
     2101 agggcgctgt tgctaattct tgcggcctga ttgtcgctgc cgatcttttt atcccaggta
     2161 atggcatccc ggttgatagc gttgaaatag atatgtccgt cgacccgcat aaagcccgtg
     2221 aaatgggggc taaggcaatg aaattacttg tactctggcg tgaagatgaa cccgcagagg
     2281 aacgtctggc aatggttgat aaatttgttc gtcgctgccg tagtgccggg ctggtgagca
     2341 ttattgaacc tgtggtgcgt ccacctcgtc gcggttggga ctttgatcgt gaaagcacca
     2401 ttgttgccgc cgccgcagag cttggtggaa cagaggctga tctttacaaa gccgagatgc
     2461 cattaggggg caaaggcgat gaaaaaacct tacttgccgc ctgtcagcaa cttaacgatc
     2521 agatgaagat gccgtgggta attctttctt ctggcgttga tgccgatatt tttggccgtg
     2581 cagtaagtat tgccatgaaa ggtggtgcgt cgggcttcct ggctggacga gcggtttggg
     2641 cgtcggttgt tggtgcacaa gatccacaaa ctatgctgcg cgatgtctct gttcctcgtt
     2701 tacaacggtt ggcagaaatt gtcgatgaag gtatcgcgca acgctaatcc cctatggata
     2761 tcgattctgg agaataacaa tggataaaat tacccaggtt ttattttcag atattgggaa
     2821 agtcaccact caatatgttg aagtaccaca ccaggaactt aaaccgcacg aagtgcggat
     2881 tgcgcctgtg ttctatggga tatgcggttc ggatctgcat gttctgaaag gcggtcatcc
     2941 gtttgccaaa ccacctgtcg tccccggtca tgaaattgca gcgcgcgtta cggaagttgg
     3001 cagcgacgtt aaaaatgtac agccgggcga tcatgttgtg gtcgatccca tcatggcttg
     3061 catggaatgc cgagcctgca aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc
     3121 tggttttcgc gcaccgggct ttgctcgctc acaacacatt gttcctgcgc gtaattgcca
     3181 tgtcgcacca gcctctttac cgctaaaaat gttggccttt gccgaaccgg cggcttgtgc
     3241 ccgtcactgc gttaaccgaa tgccgaaagc ttctctggaa agcgtactgg taattggtgc
     3301 cggaacgata ggcttatcca tcgtgcaggc actgcgcatt atgggggcag gtaagattac
     3361 cgtgattgaa cctgacgctg ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt
     3421 ttgggcacca ggtgagctgg ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc
     3481 agcgcaggcc acgcttaacg atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg
     3541 catgggcgta ccaagtgggc cgcgtgaaat accattaccg atgatgcaac gtttcgagcg
     3601 tgacttgctc aactctggca tgtacatccc tgaagatttc gatgttgtta tcgaatggct
     3661 ggcggatggg cggtttgata ccagtgaact ggttaccgat ttatttgcca ttgaggatgc
     3721 agcggcggca tttgaacgcg cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc
     3781 gccggaatga ttttctaaat ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac
     3841 aattaaaaag gacactgcga tgagtcaaat caccagtcct gcaacttatt caattagtcg
     3901 gccacaggac gtaattgata ttgttaataa gaactctgca atcaacacca gtatcggcgt
     3961 tatttttatt gctttgggcg gcatattgat tgatgcctac caggcggcga tggtggggtt
     4021 tggtaataaa tacattgccg ctcaattcgg catttcgccg ggtcttgctg caaccgtcaa
     4081 tgcatcagta ttaatcgccg cgttaattgg cggtttatta gcgaaccgag taataaaccg
     4141 ctttgggcaa aagcgggcat ttattattgg catggggctg tgcaccatcg gtgctgcagc
     4201 ggtagctatt gctcccagta tctggtgggt gctggtgtgc cgcgtcatca tgggctttgg
     4261 tttaggcatt gacttccctt tggcaaccaa tgccgtggca gagcttcgtg gttcaacgtc
     4321 gaagaaaacc ggaacgtcgg tcaacctctg gcaaatggcc tggtatgttt cgacaaccgt
     4381 tgtttatttg gtacttttgc cgctgcttct gtcgggtatc gctgaagaac aattgtggcg
     4441 ttacggaata ttcatcggag ctatttttgc agtcatcttc atgattttgc gttacttctt
     4501 tattggtgaa tccgcaatgt gggccgcacg cgtcgggcgt taccaggaag cgtgcgacat
     4561 tctgggaaaa cgttatggtg ttcaggctcg cgttgcggca tcgggtacaa cagaagcgaa
     4621 attctcggaa aaagcagaga ataaatacag tggtggatat ggcatcttat ttaatgatcg
     4681 ttaccgcaaa cgcaccattc ttggctgtgt cgtggcaacc atgcaggcgt ggcaatataa
     4741 cgccgtaggt gtttatcttc ctcttacgtt ggcgggaata ataagtggcg ggcttactgg
     4801 cgcgttgacc ggttctgccg tcgtgaatgc cctttgtggg gtgacagggg ggatgatcgg
     4861 ctcgtttatt ctccaacgac tgggtactcg ccgacagtcg atgtatggat ttgctgttgt
     4921 gaccttagca ttgctgtcgt taggcgcact ggcaacgact aatccctggc tgtctttagg
     4981 gttattggga tcaattattt tcttccattc agcgggtcct ggtgggctgg gcatgaccat
     5041 tgccacactc tcttatcctc ccgctattcg ccctactggg gtcggatttg cccgcgctat
     5101 tatgcgcaca ggggcaattg caggactcat tttctggccg atgctgtggg gtgcgttgaa
     5161 aactgaagcg ttttactggt tggcaatcgt gccattcctg gggttcctga cctgcgtatt
     5221 gattaattgg gaaccactgg gtgcaaatgt tgatgctgag gatgcagagg ttctggctga
     5281 attgaagaaa taaattaatg cgcatcggct taaaccgatg cgcattaatt caaactgccc
     5341 acgtttagtg gttcgtatcc cagatttctc gcctaccagg agaggaaaat tatgtctgaa
     5401 aagttacccg caccgcgtga agggctttcc ggtaaagcca tgagacgtgt cgttatgggc
     5461 agtttcgcag gcgcgttaat ggaatggtat gacttcttta tttttggcac ggcagcagga
     5521 ctggtatttg caccgctgtt ttatccagac agcgatcctt ttataggtct tatagccgcc
     5581 tttgcgacct ttggtgtcgg ttttctgact cgtcctttag gtggcatcgt ctttggtcat
     5641 tttggcgata aaatcgggcg aaaaataaca cttatctgga cgctggctat tgtcggttgc
     5701 tccacatttt tgattggttt tattccgacg tatcaggaaa ttggcatttg ggcaccgctt
     5761 gtattaatgg tgctgcgttt gattcagggc tttggtcttg gtggcgagta cggcggagcg
     5821 gcattgatga ccatagaatc agcgcccgaa tcccgacgcg gatttttagg ttcactcccg
     5881 caaacggctg cgtctgtggg tattatgctg gcaaccggta tttttgctct ctgtaaccat
     5941 tttcttactt cggagcagtt tctctcctgg ggatggcgca ttccgttttg gctttctgcg
     6001 gtgatgttga tcgttgggct gtttatccgc ctgcataccg aagaaacact ggattttcaa
     6061 aagcaaaaaa cgacgaataa taaagaaaag tccgttcctc cgttgattga attattcaaa
     6121 aaacatccac gaaatatttt attggcactg ggtgcgaggc tggcggaaag tgtctcctct
     6181 aatattatta acgcctttgg tattgtctat atttccagcc aattagcatt gtcgcgagat
     6241 attcccctga cgggtatgtt gattgcctcg gcgatcggta ttttcagttg tccattggtt
     6301 ggatggttat cagatcgtat aggtcaaaaa agtctgtatt tgtcgggagc aggattttgt
     6361 gtcctgttcg cgtttccttt cttcttatta ctggatagca aaagtacgct catcatctgg
     6421 tgcagtatga tcctcggcta caacttaggt ccgacgatga tgtttgccgt gcagccaaca
     6481 cttttcaccc gcatgttcgg taccaaagta cgatataccg gcctttcttt tgcttatcaa
     6541 ttttccgcga ttcttggcgg actaagcccc cttatcgcct caagtttgct ggcgctaggg
     6601 ggaggaaaac cgtggtatgt cgctctgttt ttatttgctg tctcagttct ttcctttgtt
     6661 tgcgtctggc tgattgagcc gaccgatgaa caagaaaccg cttcttaccg ctacatcagg
     6721 gaacaatctc atgaaaactg aacgaaaaaa cgagcattac ctggcgctac aacaggcgtt
     6781 tgacgcgcca tggcctgggc ctgtaggaga gttagtgacg ctggaaaaag gcaatattca
     6841 cctgcagatt tatccgcatg atggtgccag gataacctcg ttaaaggcat ttggttctga
     6901 agtattacgc caatggcagc cgcagcgcag agcttttcag tatggctgtt tcccaatggt
     6961 gccgtgggca gggcgactgg ggaatgcgac gctgaatgct ggtgggcaat gttattcttt
     7021 acccgccaat aaaccaccac atgcgttgca tggtatggct tgttactcaa cgtgggaaat
     7081 catcgacaaa acagcagatt ccctgacttt acgtatgccc ctggcttcac cgtggccctg
     7141 gcaaggggaa gtgattcaga cttttttgct ggagaacgat gcgctggttt tgcaactgga
     7201 agtccattca tatgccgata cattcccggc ctccgcaggc tggcatccgt ggtttgcgaa
     7261 aaagctgact ccgcaaaata cagaatcgtt gcaggtgctt tttgatgcgg actggcaaga
     7321 agaagcgggt agtgatgaac tgccaactgg aaatcgtatc tctcctcaag ccggtccctg
     7381 ggatgactgt tttggttttt ataacggggt gaaggttaag ttactgtggc ctggtaaact
     7441 cgcgatgacc atgatctctt ctgcaaacag tctggtggta tttgataaac agcccgatgc
     7501 cacctgtatt aacccgctga ctcaggcacc taacgcaata aatctaacgc cggaattggt
     7561 gacaccagat aggccgctgg tcatcgaaac tcgttggcaa tttacacctg aatcttaacc
     7621 attcatctat tgcgaaaaag ccagtgaaat tactctgctt tttcgcaatc agcatcacaa
     7681 gattaccctt attgccacat agccaaaccc atcctttccc gctacagtta atttcttgtg
     7741 gcgcgaaagg aggcaaaatg ctctatatct ttgatttagg taatgtgatt gtcgatatcg
     7801 actttaaccg tgtgctggga gcctggagcg atttaacgcg tattccgctg gcatcgctta
     7861 agaagagttt tcatatgggg gaggcgtttc atcagcatga gcgtggggaa attagcgacg
     7921 aagcgttcgc agaggcgctg tgtcatgaga tggctctacc gctaagctac gagcagttct
     7981 ctcacggctg gcaggcggtg tttgttgcgc tgcgcccgga agtgatcgcc atcatgcata
     8041 aactgcgtga gcaggggcat cgcgtggtgg tgctttccaa taccaaccgc ctgcatacca
     8101 ccttctggcc ggaagaatac ccggaaattc gtgatgctgc tgaccatatc tatctgtcgc
     8161 aagatctggg gatgcgcaaa cctgaagcac gaatttacca gcatgttttg caggcggaag
     8221 gtttttcacc cagcgatacg gtctttttcg acgataacgc cgataatata gaaggagcca
     8281 atcagctggg cattaccagc attctggtga aagataaaac caccatcccg gactatttcg
     8341 cgaaggtgtt atgctaaaaa ccattcagga caaagccagg caccgtaccc gtccactatg
     8401 ggcctggcta aaactactct ggcaacgtat tgatgaggac aacatgacaa ccctggcagg
     8461 taaccttgcc tatgtgtctt tgctctcatt agtgccgctg gttgccgttg tttttgcgct
     8521 tttcgccgct tttcccatgt tttccgacgt cagcattcag ttacgtcact ttatttttgc
     8581 caactttctg cctgctactg gcgatgttat tcagcggtat atcgaacaat ttgttgccaa
     8641 ttccaacaag atgaccgccg ttggggcgtg cgggctgatc gtcaccgcgt tattgttgat
     8701 gtactccatc gatagcgcgt tgaataccat ctggcgcagt aaacgagcgc gaccaaaaat
     8761 ttactcgttt gccgtgtact ggatgatctt aacgctgggg ccgctgctgg caggggccag
     8821 tctggcgatc agttcctatt tgttctccct gcgctgggcg agcgatctca atactgtcat
     8881 cgataacgtg ctgcgtattt ttccgctgct gttgtcgtgg atctccttct ggttgctgta
     8941 cagcattgtt cctaccatcc gcgtacctaa ccgcgacgcg attgtcggcg cgtttgtcgc
     9001 tgcactcctg ttcgaagcag gaaagaaagg tttcgcgctt tatatcacca tgttcccgtc
     9061 atatcagctc atttacggcg tgctggcggt gatccccatt ctcttcgttt gggtctactg
     9121 gacatggtgt atcgtcttgc ttggcgcgga aattactgtc actctcgggg aataccgcaa
     9181 actaaaacaa gcagctgaac aagaagaaga cgacgaacca tgattgcatt aattcaacgc
     9241 gtaacccgtg ccagcgtcac cgtggaggga gaagtgacgg gcgaaattgg cgcgggactt
     9301 ttggtgttat tgggtgtcga aaaggatgac gacgaacaga aagcaaaccg tctgtgcgag
     9361 cgtgtgctcg gctaccgcat ctttagcgat gccgaaggca agatgaatct caacgtgcaa
     9421 caggcgggcg gcagtgtact ggtggtttcc cagtttaccc tcgccgcaga taccgaacgg
     9481 gggatgcgcc caagtttctc caaaggcgca tcaccggatc gcgcagaggc gttatatgac
     9541 tatttcgtcg aacgctgccg ccagcaagag atgaacacgc aaacaggacg cttcgctgcc
     9601 gatatgcagg tatcgctggt caatgatggc cccgtgacat tctggttgca ggtatgagcc
     9661 tgcttccagg gttgtcacgg gaaacaagag agagtatcgc tatgtatcac cttcgggttc
     9721 cacaaacaga agaagagtta gagcgttact atcagtttcg ctgggaaatg ttgcgtaagc
     9781 ccctgcatca accaaaaggt tcggaacgcg acgcgtggga tgcgatggcg catcaccaga
     9841 tggtcgtcga cgagcagggt aatctggtgg cggtaggccg attgtatatt aatgccgaca
     9901 atgaagcgtc cattcgcttt atggccgttc acccggacgt gcaggacaaa gggttaggaa
     9961 cgctgatggc gatgaccctg gagtcggtgg cgcgtcagga aggcgttaag cgcgtgacct
    10021 gtagcgcccg tgaagacgcg gtggagtttt tcgccaagct gggattcatt aatcaggggg
    10081 agatcaccac cccaactacc acgccgattc gccatttttt gatgattaaa cccgttgcca
    10141 ctctggatga cattctgcat cgcggtgact ggtgcgcgca gctgcaacag gcgtggtacg
    10201 aacatatccc gttaagcgaa aaaatgggcg tgcgcattca gcaatatacc gggcaaaaat
    10261 ttatcaccac catgccagaa accggtaatc agaatccgca ccatacgttg tttgccggga
    10321 gtttattctc gctggcgacg ctcaccggtt ggggacttat ctggctgatg ctgcgtgaac
    10381 gccacctcgg cggaacgatt attctggcgg atgcgcatat ccgctacagc aagccgatta
    10441 gcggtaaacc tcatgcggta gccgaccttg gtgccttaag cggcgatctc gaccgtctgg
    10501 cgcgcggacg aaaagcacgg gtgcagatgc aagttgaaat ctttggcgac gagacgccgg
    10561 gtgcagtgtt tgaaggcacg tatatcgttc tgcccgcgaa gccatttggc ccgtatgaag
    10621 agggcgggaa cgaagaagag tagcttatgg agaggggcgg tgctgcctct ctcattcatt
    10681 ccggt
//
LOCUS       DAESHI010000140        10526 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.142, whole genome shotgun sequence.
ACCESSION   DAESHI010000140 DAESHI010000000
VERSION     DAESHI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10526
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.142"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(71..1099)
                     /gene="malI"
                     /locus_tag="KW011_003700"
     CDS             complement(71..1099)
                     /gene="malI"
                     /locus_tag="KW011_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HBH7661941.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1274..2866
                     /gene="malX"
                     /locus_tag="KW011_003701"
     CDS             1274..2866
                     /gene="malX"
                     /locus_tag="KW011_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HBH7661942.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2876..4048
                     /gene="malY"
                     /locus_tag="KW011_003702"
     CDS             2876..4048
                     /gene="malY"
                     /locus_tag="KW011_003702"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HBH7661943.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4152..5153
                     /gene="add"
                     /locus_tag="KW011_003703"
     CDS             4152..5153
                     /gene="add"
                     /locus_tag="KW011_003703"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBH7661944.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5264..6229)
                     /locus_tag="KW011_003704"
     CDS             complement(5264..6229)
                     /locus_tag="KW011_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBH7661945.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPAS"
     gene            6473..6598
                     /gene="blr"
                     /locus_tag="KW011_003705"
     CDS             6473..6598
                     /gene="blr"
                     /locus_tag="KW011_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HBH7661946.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6863..7078
                     /gene="ydgT"
                     /locus_tag="KW011_003706"
     CDS             6863..7078
                     /gene="ydgT"
                     /locus_tag="KW011_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HBH7661947.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7164..7604
                     /locus_tag="KW011_003707"
     CDS             7164..7604
                     /locus_tag="KW011_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HBH7661948.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7681..8262
                     /gene="rsxA"
                     /locus_tag="KW011_003708"
     CDS             7681..8262
                     /gene="rsxA"
                     /locus_tag="KW011_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBH7661949.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8262..8840
                     /gene="rsxB"
                     /locus_tag="KW011_003709"
     CDS             8262..8840
                     /gene="rsxB"
                     /locus_tag="KW011_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HBH7661950.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8833..>10526
                     /gene="rsxC"
                     /locus_tag="KW011_003710"
     CDS             8833..>10526
                     /gene="rsxC"
                     /locus_tag="KW011_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBH7661951.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIAR"
BASE COUNT         2405 a         2720 c         2692 g         2709 t
ORIGIN      
        1 ctgtgtgcca cattttttat cattaacctt gaggaaagga cactaccaag tatagcagat
       61 aaagagagga ttatttcgct gcaatgagcc gggcgggaat aataagattg cgtgaatgcg
      121 tctcttcatg ggtgattttt tgcatcatgc gatccgcaag tgtggtacca agttcccgcg
      181 caggcgtgct ggcccaggta acgggtatat catcaagtgt ggttggtgtc gcatcggtaa
      241 atgccgccag cgaaacttgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc
      301 gccctgcttt cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca
      361 cagcactgat ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct
      421 ggctggatgt gcactccagc acccaatcgc tgtgaaacgg caggccaaat tttagtagag
      481 ttgcacaata gcctcccacc cgttctgccc gggttaatga ggaactttgc cctcccagcc
      541 aggcgatccg ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca
      601 tgttgtccgg gcgaaccgta tcaacatcat caagataact ggcacgggaa gcgaaaatca
      661 ccgggatagc tttttcttct gccatccgtc gcaggtcatc gctgcttcct gcagccccgg
      721 caattaccac accatcgaca ccctgattca gtaacagtga aaaccgctgt gccagctgct
      781 cgccgtcttt accgccgtga agcaaaaaaa ccatccgtcc ctgcgcttcc agagcttccg
      841 tcaatccggc cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa
      901 tgacgccgct ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca aatcccagct
      961 cttcaatggc ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccactaa
     1021 gcaccagcga aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta
     1081 tttttttggc ggtagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt
     1141 ttacctttta tttatcttat acccgctatt atcgttgcgt aatgtgattt atgcttcact
     1201 aaaatttgat aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac
     1261 gaggagtcgt tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca
     1321 gcagttaggc aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct
     1381 cggcattggt agttctctta gtagccacga tgtcatcacc ctgatcccgg tcctgggcaa
     1441 ccccgtgttg caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt
     1501 cctgccagtc atgttctgta tcgccatccc gctgggtctg gcacgcgaaa acaaaggcgt
     1561 agcggcattc gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt
     1621 gaccaataaa ggcattctgc caaccacgga cgccgcggtt ctgaaagcca ataacatcca
     1681 gagcattctt gggatccagt cgatcgacac cgggatcctc ggtgcggtga tcgccggtat
     1741 tatcgtctgg atgctgcatg agcgattcca taatatccgc ctgccggatg cgcttgcatt
     1801 cttcggcggt acgcgcttcg taccaatcat ctcctcgctg gtgatgggcc ttgtcggcct
     1861 ggtgattcca ttagtctggc cgattttcgc gatgggtatt agcggcttgg gccatatgat
     1921 aaacagcgcg ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttgcc
     1981 gtttggtctg catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca
     2041 ggaagtctgc ggtcaaaccg tcagcggcgc attgaccatc ttccaggcgc aattgagttg
     2101 cccgaccact cacggttttt ctgaaagcgc cacgcgtttc ctttcgcaag gcaaaatgcc
     2161 tgcgtttctc ggcggtctgc caggtgcagc gttagcgatg tatcactgcg cgcgcccgga
     2221 aaatcgccat aaaattaaag ggctgctgat ttctggcctg atcgcctgtg tcgttggcgg
     2281 cacgaccgaa ccgctggaat tcctgttcct gttcgtggcg ccagttctgt atgtcatcca
     2341 cgcgctgtta accggcctcg gcttcactgt catgtctgtg ctcggcgtca ccatcggtaa
     2401 taccgacggc aatatcatcg acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa
     2461 gtggtacatg gtgccagtgg tggcggcaat ctggtttgtc gtttactacg tcatcttccg
     2521 tttcgctatc acccgcttca atctgaaaac cccggggcgc gatagcgaag tggccagttc
     2581 aatcgaaaaa gccgttgccg gtgcgccggg taaatcaggt tacaacgttc cggcaatcct
     2641 cgaagcctta ggcggtgccg acaatattgt tagcctcgat aactgcatta cccgtctgcg
     2701 tttgtctgtg aaagatatgt cgcttgttaa tgtgcaggca ctgaaggaca atcgggcaat
     2761 tggcgtagta caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc
     2821 agtaaaagat gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt
     2881 cgatttttca aaggtcgtgg atcgtcatgg cacatggtgt acacagtggg attatgtcgc
     2941 tgaccgtttc ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac
     3001 tgccccctgc attatcgagg cgctgaatca gcgcctgatg cacggcgtat ttggctacag
     3061 ccgctggaaa aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta
     3121 caccgcaatt gatacgcaga cggtggtgta tggcccttct gtcatctata tggtttcaga
     3181 actgattcgt cagtggtctg aaacaggtga aggcgtggtg atccacacgc ccgcctatga
     3241 cgcattttac aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa
     3301 gcaggctgac ggctggtttt gcgatatggg caagctggaa gccgttttgg cgaaaccaga
     3361 atgtaaaatt atgctcctgt gtagcccaca gaatcctacc gggaaagtgt ggacgtgcga
     3421 tgagctggag atcatggctg acctgtgcga gcgtcatgat gtgcgggtta tttccgatga
     3481 aatccatatg gatatggtct ggggcgagca gccgcatatt ccctggagta atgtggcgcg
     3541 gggagactgg gcgttgctga cgtcgggatc gaaaagtttc aatattcccg cgctgaccgg
     3601 tgcttacggg attatagaaa atagcagcag ccgtgatgcc tatttatcgg cactgaaagg
     3661 acgtgatggg ctttcttccc cttcggtact ggcgttaact gcccatatcg ccgcctatca
     3721 gcaaggtgca ccgtggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat
     3781 ctcagataaa atgaacgccg cgtttcctga actcaactgg cagatcccgc aatccactta
     3841 tctggcctgg cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct
     3901 tatcgagcaa gaaaaagtcg cgatcatgcc ggggtatacc tatggtgaag aaggtcgtgg
     3961 ttttgtccgt ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt
     4021 aataaacgcc atccgcgctg ttcgctaacc ccaattgcgc aacgtaaaaa atcgttgcgc
     4081 aatcgtggat ttttaccctg ctttgttttt ataatggtgc gcacttttat atccagaaaa
     4141 agagtgcgac catgattgat accaccctgc cattaactga tatccatcgc caccttgatg
     4201 gcaacattcg tccccagacc attcttgaac ttggccgcca gtataatatc tcgcttcctg
     4261 cacaatccct ggaaacactg attccccacg ttcaggtcat tgccaacgaa cccgatctgg
     4321 tgagctttct gaccaaactt gactggggcg ttaaagttct cgcctctctt gatgcctgcc
     4381 gccgcgtggc atttgaaaac attgaagatg cagcccgtaa cggcctgcac tatgtcgagc
     4441 tgcgtttttc accaggctac atggcaatgg cacatcagct gcctgtagcg ggtgttgtcg
     4501 aagcggtgat cgatggcgta cgtgaaggtt gccgcacctt tggtgtgcag gcgaagctta
     4561 tcggcattat gagccgaacc ttcggcgaag ccgcctgtca gcaagagctg gaggcctttt
     4621 tagcccaccg tgaccagatt accgcacttg atttagctgg tgatgaactt ggattcccgg
     4681 gaagtctgtt cctttctcac ttcaaccgcg cgcgtgatgc gggctggcat attaccgtcc
     4741 atgcaggcga agctgccggg ccggaaagca tctggcaggc gattcgtgaa ctgggtgcgg
     4801 agcgtattgg acatggcgta aaagccattg aagatcgggc gctgatggat tttctcgccg
     4861 agcaacaaat tggtattgaa tcctgtctga cctccaatat tcagaccagc accgtagcag
     4921 agctggctgc acatccgctg aaaacgttcc ttgagcatgg cattcgtgcc agcattaaca
     4981 ctgacgatcc cggcgtacag ggagtggata tcattcacga atataccgtt gccgcgccag
     5041 ctgctgggtt atcccgtgag caaatccgcc aggcacagat taatggtctg gaaatggctt
     5101 tcctcagcgc agaggaaaaa cgcgcactgc gagaaaaagt cgccgcgaag taacaaaaat
     5161 ggatggtgca atttgcacca tccatttttc acgcaaggca caacgtcgcg cgatgtttgg
     5221 cggattcgat gcccagctca atcaactcca ttacctggat tgcctagctt gccggaaccg
     5281 gattttcacc atcgccattt aacgcatcac gaatcgccgc atagtaagcc ggataattcc
     5341 caggcaccgt caacagcgtt tcttcgacac gttcctcacc ttccacgcgg gtaagtacgc
     5401 catcacgcat atcgtagccc cagtcttcct gcggcagacg ctcgccattt ttcagacgtt
     5461 cttcctgtgg atcgaggcca tatttcacat aactgcctcg ggatccatgc acgatatacc
     5521 gtgctgactc agcggctgcc agcatggtac cgtgtaaaat gactcgccgc tgtggatagg
     5581 acaagatggc gtggaaataa tcggtcgact gcgctccggg ccgtaactgt gccaaatcta
     5641 ccgtcatgct gaccggtaaa ccaaatagcg taatggcctg atcaagaaga tgcggtgcta
     5701 aatcgtacca gataccgctg cctgggccgc cctgttcacg ccaacgatcg cgcacctgcg
     5761 gacggaagcg gtcaaaatga gactcaaagt aagcaacttc acccagcacg ccttccgcga
     5821 gcaaaccttt tagcgtcaag aaatcgctat cccaacgacg gttatggaat acagacagca
     5881 cacgccccag gctttttgcc agcgcatcca gctctcgcgc ttgtgacagt gtcacggtaa
     5941 agggtttatc aacgaccaca tgtttacccg cctcaagcgc cgctttggct aacgggaaat
     6001 gggtatcgtt gggtgtagga atgacaatca ggtctatgtt gggatcgtta aacagatgct
     6061 tcggctcaga gacaaccgta accgttggcc agtcggcttt tacttttgtt tcatcactgc
     6121 tggagattac cgccagttcc agcccgggcg tgcccgcaat caggggcgca tggaaggttt
     6181 tgctcgcata accataccca atcaacccaa cacggatgtt gtcgctcatg tcattgcctc
     6241 tcattgaagt atgatggcta tttgacacta tcctttaccc acgctcaaca gtttaataac
     6301 ctgccagcaa taagggatgt tgtttaactt aagtcaaaaa aaatagcgaa ttttccaacg
     6361 acaaaagcta aatatcgcaa aaacctcagt aaaaatcttg ctggagctat tattgctaag
     6421 taacatttac cccctgaagt taatggatca atcaagagag atgtgggctg taatgaatcg
     6481 tcttattgaa ttaacaggtt ggatcgttct tgtcgtttca gtcattcttc ttggcgtggc
     6541 gagtcacatt gacaactatc agccacctga acagagtgct tcggtacaac acaagtaagc
     6601 tctgcacttg tggagcgaca tgctgcccgt ccgggtgcat gttttcactt gtcggatatt
     6661 aaaccaggaa tttattatct tgttcgatgt tgttggtgat tgtcagggat agtaaagcgc
     6721 gttactcttt tcagagcagt ttttcatccc tgaatatttg ccatcagcgc attcttatta
     6781 ttgtaataac acaagcgttc acttttggtg aaattttatc atcactctac ccctttggta
     6841 ttaattattt tatggacctt ttatgactgt tcaggactac ttattaaaat ttcgcaaaat
     6901 cagttcactc gaaagtctgg aaaaactcta cgaccatctt aattacaccc tgacggacga
     6961 tcaggaactg atcaatatgt atcgtgctgc cgatcaccgt cgcgcagagc tggtttctgg
     7021 cgggcgtttg tttgacctcg gccaggtacc gaagtccgtc tggcactatg tccaataaag
     7081 aaagtagcga tcggttataa aagccttata ataacgcccc taaaattaga tttgcctgtt
     7141 tgcgtcgcta ttctggagaa cctatgacca ccacaacgcc acaacgtatt ggaggctggt
     7201 tgcttggccc gttggcttgg cttttagtcg ctctgttgag tacgacgctg gcgttgttgc
     7261 tgtataccgc tgcgttatct tctccgcaaa catttcaaac gctcggcgga caagcactga
     7321 cgacacaaat cttatggggc gtttctttta ttaccgctat cgccatgtgg tattacacac
     7381 tgtggttgac catcgctttc tttaaacgtc ggcgttgtgt tcctaaacac tatattatct
     7441 ggctgcttat ttccgtattg ctggcagtaa aagcattcgc tttttcaccc gtggaagatg
     7501 gcatagccgt tcgccagttg ctgtttactt tgttggcaac cgcattgatc gtcccctatt
     7561 tcaaacgctc gtcgcgggta aaagcgacgt ttgtgaatcc gtaataacct tacagttaac
     7621 ctgttgtcgc ctgctctgga ttaacggata ataggcggct tttttatttc aggccgaaaa
     7681 atgactgact acctgttact gtttgtcgga actgtactgg tcaataactt tgtactggtc
     7741 aagtttctcg gtctctgtcc gtttatgggg gtttccaaaa aactggaaac cgcgatgggc
     7801 atggggctgg caacaacgtt tgtgatgacg ctggcgtcta tttgcgcctg gcttatcgat
     7861 acgtggattt tgatcccact taatctgatt tacctgcgca ccctggcatt tattctggtg
     7921 attgctgtgg tcgtgcagtt caccgagatg gtggtgcgca aaaccagccc ggtgctttac
     7981 cgtctgctgg ggattttttt gccgcttatc accaccaact gtgcagtgct cggcgtggcg
     8041 ttgctgaata tcaatctcgg gcacaatttc ttgcagtcgg cgctgtacgg tttttccgcc
     8101 gctgtcggtt tctcgctggt gatggtgctc ttcgccgcca tccgcgaacg ccttgctgtg
     8161 gctgatgtcc cggcaccttt tcgcggtaat gccattgcgt taattaccgc aggtcttatg
     8221 tctctggcct ttatgggctt taatggtttg gtgaagttgt aatgaatgct atctggattg
     8281 ccgttgccgc cgtgagcctg ctgggcctgg cgtttggcgc cattctgggt tatgcctccc
     8341 gccgttttgc ggtggaagac gatccggtcg ttgagaaaat tgacgaaatc ttaccgcaga
     8401 gccagtgtgg tcagtgcggt tatcccggct gtcgccccta cgcggaagcc atcagctgta
     8461 acggtgaaaa aatcaaccgt tgcgccccag gtggcgaagc tgtgatgcta aaaattgccg
     8521 agttgcttaa tgtcgagccg cagccgctgg atggcgaagc gcaagagcta acgcctgcgc
     8581 ggatggtggc ggttattgat gaaaataact gtattggctg cactaaatgt attcaggctt
     8641 gtccggtaga cgccatcgtt ggcgctaccc gtgccatgca tacggtaatg agtgatctct
     8701 gtacgggctg caatttatgt gttgatccgt gcccgacgca ctgcatctcg ttgcaaccgg
     8761 tcgcagaaac acctgactcc tggaaatggg atctgaacac cattcccgtg cgtatcattc
     8821 ccgtggaaca ccatgcttaa gttattctct gcattcagaa aaaataaaat ctgggatttc
     8881 aacggcggca tccatccacc ggagatgaaa acccagtcca acggtacacc cctgcgccag
     8941 gtaccactgg cgcagcgttt tgttattcca ctgaaacagc atattggcgc tgaaggtgag
     9001 ttgtgcgtta gcgtcggcga taaagtattg cgcggccagc cgcttacccg tggtcgcggc
     9061 aaaatgctgc ctgttcacgc gcccacctcg ggtaccgtta cggctattgc gccccactct
     9121 acggctcatc cttcagcttt agctgaatta agcgtgatta ttgatgccga tggtgaagac
     9181 tgctggatcc cgcgcgacgg ctgggccgat tatcgcactc gcagtcgcga agagttaatc
     9241 gagcgcatac atcagtttgg tgttgccggg ctgggcggtg caggattccc gacaggcgtt
     9301 aaattgcagg gtggcggaga taagattgaa acgttgatta tcaacgcggc tgagtgcgag
     9361 ccgtacatta ccgccgatga ccgtttgatg caggattgcg cggctcaggt cgtagagggt
     9421 attcgcattc ttgcgcatat tctgcagcca cgcgaaattc ttatcggcat tgaagataac
     9481 aaaccgcagg cgatttccat gctgcgcgcg gtgctggcgg actctaacga tatttctctg
     9541 cgggtgattc caaccaaata tccttctggc ggtgctaaac aattaaccta cattctgacc
     9601 gggaagcagg ttccacatgg cgggcgttca tccgatatcg gcgtattaat gcaaaacgtc
     9661 ggcactgctt atgcagtgaa acgtgccgtt attgatggcg agccgattac cgagcgtgtt
     9721 gtaaccctga ctggcgaagc aatcgctcgc ccgggcaacg tctgggcacg gctggggacg
     9781 ccagtgcgtc atttattgaa tgatgccgga ttctgcccct ctgccgatca aatggtgatt
     9841 atgggtggcc cgctaatggg ctttaccttg ccatggctgg atgtcccggt cgtaaagatt
     9901 accaactgtc tgttggctcc ctctgccaat gaacttggcg aaccacagga agaacaaagc
     9961 tgcatccggt gtagcgcctg tgctgacgcc tgccctgcgg atcttttgcc gcaacagttg
    10021 tactggttca gcaaaggtca gcaacacgat aaagctacca cgcataacat tgctgattgc
    10081 attgaatgtg gggcttgcgc gtgggtttgc ccgagcaata ttcccctggt gcaatatttc
    10141 cgtcaggaaa aagctgaaat tgcggctatt cgtcaggaag aaaagcgcgc cgcagaagcc
    10201 aaagcgcgtt tcgaagcgcg ccaggctcgt ctggagcgcg aaaaagcggc tcgccttgaa
    10261 cgacataaga gcgcagccgt tcaacctgca gccaaagata aagatgcgat tgctgccgct
    10321 ctggcgcggg tgaaagagaa acaggcccag gctacacagc ctattgtgat taaagcgggc
    10381 gaacgcccgg ataacagtgc aattattgca gcacgggaag cccgtaaagc gcaagccaga
    10441 gcgaaacagg cagaactgca gcaaactaac gacgcagcaa ccgttgctga tccacgtaaa
    10501 actgccgttg aagcagctat cgcccg
//
LOCUS       DAESHI010000141        10455 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.143, whole genome shotgun sequence.
ACCESSION   DAESHI010000141 DAESHI010000000
VERSION     DAESHI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.143"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="KW011_003711"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="KW011_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HBH7661952.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="KW011_003712"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="KW011_003712"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBH7661953.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="KW011_003713"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="KW011_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HBH7661954.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="KW011_003714"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="KW011_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBH7661955.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="KW011_003715"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="KW011_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="KW011_003716"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="KW011_003716"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBH7661956.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="KW011_003717"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="KW011_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HBH7661957.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="KW011_003718"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="KW011_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HBH7661958.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="KW011_003719"
     CDS             complement(9457..10149)
                     /locus_tag="KW011_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBH7661959.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2556 a         2522 c         2763 g         2614 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtgggtcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    10321 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg
    10381 ttctcctgag aatctcaaca gagaaagcaa aaataaatgc ttgactctgt ggcgggaaag
    10441 cgtattatgc acacc
//
LOCUS       DAESHI010000142        10343 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.144, whole genome shotgun sequence.
ACCESSION   DAESHI010000142 DAESHI010000000
VERSION     DAESHI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10343
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.144"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(42..1046)
                     /gene="cra"
                     /locus_tag="KW011_003720"
     CDS             complement(42..1046)
                     /gene="cra"
                     /locus_tag="KW011_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HBH7661960.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1128..1214)
                     /locus_tag="KW011_003721"
     CDS             complement(1128..1214)
                     /locus_tag="KW011_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HBH7661961.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1226..1717)
                     /gene="ilvN"
                     /locus_tag="KW011_003722"
     CDS             complement(1226..1717)
                     /gene="ilvN"
                     /locus_tag="KW011_003722"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBH7661962.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1720..3444)
                     /gene="ilvI"
                     /locus_tag="KW011_003723"
     CDS             complement(1720..3444)
                     /gene="ilvI"
                     /locus_tag="KW011_003723"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HBH7661963.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3563..3607
                     /locus_tag="KW011_003724"
     CDS             3563..3607
                     /locus_tag="KW011_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661964.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3762..4706)
                     /gene="leuO"
                     /locus_tag="KW011_003725"
     CDS             complement(3762..4706)
                     /gene="leuO"
                     /locus_tag="KW011_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HBH7661965.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5366..5452
                     /gene="leuL"
                     /locus_tag="KW011_003726"
     CDS             5366..5452
                     /gene="leuL"
                     /locus_tag="KW011_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HBH7661966.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5545..7116
                     /gene="leuA"
                     /locus_tag="KW011_003727"
     CDS             5545..7116
                     /gene="leuA"
                     /locus_tag="KW011_003727"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBH7661967.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPLEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            7116..8207
                     /gene="leuB"
                     /locus_tag="KW011_003728"
     CDS             7116..8207
                     /gene="leuB"
                     /locus_tag="KW011_003728"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBH7661968.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8210..9610
                     /gene="leuC"
                     /locus_tag="KW011_003729"
     CDS             8210..9610
                     /gene="leuC"
                     /locus_tag="KW011_003729"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBH7661969.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9621..10226
                     /gene="leuD"
                     /locus_tag="KW011_003730"
     CDS             9621..10226
                     /gene="leuD"
                     /locus_tag="KW011_003730"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBH7661970.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
BASE COUNT         2710 a         2719 c         2526 g         2388 t
ORIGIN      
        1 gccagaggga aattcacctg gcgcgtattt ttgttcgcgg cttagctacg gctgagcaca
       61 ccgcggcgat agagattacg tttaatgcgc gttaaaccag gttttggctt acgcggttcg
      121 tccaggcttg ccaggacaat ctccagcaca cgctctgcga catcgcggtg acgttgagcc
      181 actgccagca ccgggcactg taagaagtcg agcagttcgt tatcgccaaa ggtggcaatt
      241 gccaggtcag aaggcagttt accatcgcga cgtagcgtga catccatcac cccttgcaac
      301 aacgcaaacg acgttgtgaa cagcgcctgc ggcatcggat gcgtttccag ccatttttcg
      361 aataactggg cagccacctc ccgctcatag ctgttggcat acaggaaatg cacttcgcgc
      421 ggatcatctt tccaggcagt acggaaacct tgttcacgca ggaagctgac agaaagctcc
      481 ggcagcgcgc caagataaag caccgtctcg gcgggaaact tacgtaactc ttccgccagc
      541 atttcggcat catcctgatc ggcaccaacc acgctggtga agtgttcacg atcgagggcg
      601 cggtccagcg cgacaatcgg gaacgggtcg ttagcccagc gttgataaaa aggatgctca
      661 ggaggcaacg acgtcgaaac aataatggca tcgacctgac gctgtaaaag gtgctcaata
      721 cagcgcattt cgttgtctgg ctgatcttct gagcaggcaa ttagcagttg ataaccccgt
      781 tgccgcgcct ggcgttcaag atagttagcg atgcgggtat agctggtgtt ctccagatcg
      841 gggatcacaa gaccaataga acgtgtgcgt ccagcacgaa gcccagctgc cacggcgttc
      901 gggtggtaat tgtgctcacg caccacagcc atgacttttt caacggtttt gtcgctcaca
      961 cggtattgct tcgctttgcc gttaataaca tagcttgcag tggtccgcga cactcccgcc
     1021 agccgagcga tttcatccag tttcacaatt gccccttgcg taaaatgtaa aaaccatagc
     1081 cattgtactg gtatgggtta aattcattaa catctaagcg cataaaatta actgcggcaa
     1141 ctgcttttat tccaggttcc cactgatttc gcaaaaaaag cccaacgtga catgttgggc
     1201 tgcaaattgc gcattcagat cattctcaac gcattatttt atcgccgcgc gaaagtccga
     1261 ccacaccaga acgagcaact tccacaattt tcgccacctc gcgaatcgat gctaaaaatg
     1321 catcaagctt atcgctggtg cctgctaatt gaacggtata aagcgagggt gtgacatcga
     1381 taatttgccc acggaatatt tccgtattac gtttcacttc gtcacgcccg taaccgctgg
     1441 cctgaatttt caccagcatg atttcccgct caacatgcgc gccctgcccc aactcactca
     1501 cgcgcaagac atcgaccagc ttgtgtaatt gcttttcgat ctgctcaagt actttttcat
     1561 cgcccacggt ctggatggtc atacgcgata atgtcggatc atcggttgac gcaacggtca
     1621 ggctttcaat gttgtagcca cgctgggaaa aaaggccaat cacgcgggat aacgcgcctg
     1681 attcattttc gagtaagact gataatatcc ggcgcataat caggttctct ccgttttgct
     1741 taaccacatt tcatccattc cgcccccgcg aatctgcatc gggtagacgt gctcgctgcc
     1801 atcgacggta acatcaacaa acaccaggcg attattgtgc acctgttcca gcgcctcgct
     1861 aagtttgctt tccagctcat gcggatgaga aatctggatc ccgacgtgac cataggcttc
     1921 cgccagacgg acgaaatcgg gtagcgattg catataagat tgtgaatgac ggccggaata
     1981 gatcatgtcc tgccactgct tcaccatccc cagatagcgg ttattgagat tcaccaccag
     2041 tacgggcaac tcgtattgca acgcggtaga cagttcctgg atgttcatct gaatactgcc
     2101 gtcgccagtg acgcaaacca cggtttcttc tggcaacgcc attttgacgc ccagtgccgc
     2161 aggtaaacca aaacccatcg tgccgaggcc accggaattg atccagcgac gcggtttgtc
     2221 gaatggataa taaagtgcag caaacatctg gtgctgcccg acatcggacg tcacgtaagc
     2281 gtctcccttc gtcaaccgcc aaagagtctc gatcaccgcc tgcggtttaa tcttttcact
     2341 gtgagtgtca tatttcaggc actgacgagc gcgccactgt tcaatttgct gccaccagtc
     2401 gcggatctca tccagtggtt gatgggcgga ttcttgcgac aagagttcaa gcatttgttc
     2461 gaggacctgg cgagcatccc ccacaatcgg gatatccgca gtcacggttt tagaaatgga
     2521 agtaggatca atatcgatat gcagaacagt ggcatttggg cagtactttg ccagattgtt
     2581 cgtcgttcgg tcatcaaatc gtaccccgac ggcgaaaatc acatccgcgt tatgcatcgt
     2641 catattggct tcgtaggtac cgtgcattcc cagcatgccc agtgcctgac gatgcgttgc
     2701 cggaaacgct cccagcccca tcaatgagga aacaacgggc agattcaacg cctccaccgt
     2761 ttctttcaac tgctgatggc agcccgccgt gattgcccca ccgcctacgt agacaaccgg
     2821 tttttttgcc gctaccagcg tttgcagagc gcgcttaatt tgccctttat gtccggtagt
     2881 agtgggattg taagaacgca tactgaccga ctccggccag acatagggta atttgttcgc
     2941 cggattaaga atatctttcg gtaaatcaac gactactggt ccaggacgac cacttgccgc
     3001 cagccagaaa gcctttttca gcacctgcgg aatgtcttcc gtttgcttaa ccagaaaact
     3061 gtgtttaacc actggtcgcg aaatccccac catgtcgcac tcctgaaagg catcgtaacc
     3121 tatcaacgag gtcgctacct gcccggaaag gacaactaat ggaatggaat ccatataagc
     3181 ggtggcgatg ccagtaatcg cattggtcgc ccctggaccc gacgttacca gcacgacgcc
     3241 gacttccccg gtcgcgcgcg ccagaccatc ggccatatgc actgccgcct gctcatgacg
     3301 aactaaaaca tgatcgatac cacccacggt atgcaatgca tcgtaaatat caaggactgc
     3361 gcctccggga taaccgaata cttgtttaac gccctgatcg ataagcgatc ggacgaccat
     3421 ctcggctcca gacaacatct ccatggcctg cctcactgtt tgacggaaaa aatgtgtaaa
     3481 acacattaac cgtccgatct tgcttaagca attggcagcc agagacataa acgcagggta
     3541 ataagaataa acaggaggaa aggtgaaaag aaacgataaa atcctccatt acgaaggatt
     3601 aaattgataa taaatcggct gaatcccaca acttacagca atttctctat ttttttaatt
     3661 aatcctaaaa tagagagccc acacattcag aataaaattc tacattcaat aaaaaataca
     3721 gaataaacca gacattcatg tctgacctat tctgcattca gttagcgttt gcaaattgag
     3781 actaattgct cttccatcca ctgatggcct ttatcgcgcc cggcagcttc atgccaggag
     3841 agataacagg ttctgctatt ttgttttaac ggcagcggta atacctgtaa ttctaaggat
     3901 tcagcgaact cttcagccag ccaacgcggc gcaatagcga ccaaatgcgt ttgcgacacc
     3961 acgctaagta cgctcatcat tgccatgccc tgatacgcga tactggcttg cttatctacc
     4021 gtgtcatacc aaggttgact aaatgacgcg aaacgatcga gcgaaaccgc cgcatgttgt
     4081 tcgttataaa catcatgttt cagtaacggg cccttaattg ttggatgatt tttgctggct
     4141 accagcacca tttcatcttt aaataatggc acgctggtaa attcaggacg atggaagtct
     4201 tcataactaa tcacaaactc cgtttcctga taacgcagct gatgttcagt gttctgattt
     4261 aatgaagact tgaacataac atgtatattt ggcgcaatct gctcaatgtg attataaatc
     4321 tgcgaggtca gaatgctgtc taacgggctg caaacacaaa gatgaaatac acgttcactg
     4381 ctcgcgggtt caaaacctga accaggcaat tcattttgta ctagttgcaa tgcctgacga
     4441 actgagccaa aaagttgaaa tgcgcgagca gtcggttgaa taccacggcc ataacgaaca
     4501 aaaagctcgt cattaaacat caccttcagg cgtgcaacag cgttactgac cgcaggttgc
     4561 gacataccca gaacatgagc ggcacgggta atgttttgct cctgcatcac ggcatcgaaa
     4621 acggttaata agttgagatc gaccatgcgt agctgtggct tgcttaactc cgccgtctct
     4681 gaatgatctg tttgtacctc tggcatactt aactccactg tcacacttaa ctccctttcc
     4741 cttattggaa tgcagagaaa taatcctgaa gaatatgatt tatcatgcat ataaaataag
     4801 aaaaagcaaa atgagtaaaa ttcggaaaac ataaagatac gggttctgat aaaaacagaa
     4861 tccattgccg tggaatgagt catttaccac agcataagaa tccataatgt ttttgttgcg
     4921 aaaacaatct aatcataact accgcgaata ctcaatcatc tacaaaatgg attaaatgtg
     4981 cttatttaat aattaattta tatatttaat gcattaattc ttaacattaa ttgatcaata
     5041 atattcacca aatcaatatc aaaaaaaatc gcaaaacata taattcaata caaatcatca
     5101 ggataggttt tgcaacgcgt gcattttgtc ccctttttcc tcgttgatta gatgcaaaaa
     5161 tttatgctga aatatgtcaa ccgatgaaaa gcgtcggtag ttaagcagaa attaatatcg
     5221 cttactttaa ccaccgcagc acaattagct aattttacgg atgcagaact cacgctggcg
     5281 ggacgttttt attgcgtcag ggttgacatc cgtttttgta tccagtaact ctaaaagcat
     5341 atcgcattca tctggagctg atttaatgac tcacatcgtt cgctttatcg gtctactact
     5401 actaaacgca tcttctttgc gcggtagacg agtgagcggc atccagcatt aagccagcac
     5461 gcagtcaaac aaaaaacccg cgccattgcg cgggtttttt tatgcccgaa gcgaggcgct
     5521 ctaaaagaga caaggaccta aaccatgagc cagcaagtca ttattttcga taccacattg
     5581 cgcgacggtg aacaggcgtt acaggcaagc ttgagtgtga aagaaaaact gcaaattgcg
     5641 ctggcccttg agcgtatggg tgttgacgtg atggaagtcg gtttccccgt ctcttcgccg
     5701 ggcgattttg aatcggtgca aaccatcgcc cgccaggtta aaaacagccg cgtatgtgcg
     5761 ttagctcgct gcgtggaaaa agatatcgac gtggcggccg aatccctgaa agtcgccgaa
     5821 gccttccgta ttcatacctt tattgctacc tccccaatgc acatcgccac caagctgcgc
     5881 agcaccctgg acgaggtgat cgaacgcgct atctatatgg tgaaacgcgc ccgtaattac
     5941 accgatgatg ttgaattttc ttgcgaagat gccgggcgta cacccattgc cgatctggcg
     6001 cgagtggtcg aagcggcgat taatgccggt gccaccacca tcaacattcc ggacaccgtg
     6061 ggctacacca tgccgcttga gttcgccgga atcatcagcg gcctgtatga acgcgtacct
     6121 aacatcgaca aagccattat ttccgtacat acccacgacg atttaggcct ggcggtcggc
     6181 aactcactgg cggcggtaca tgccggtgca cgccaggtgg aaggtgcaat gaacgggatc
     6241 ggcgagcgtg ccggtaactg ttcgctggaa gaagtcatca tggcgattaa agttcgtaag
     6301 gatattctca acgtccacac cgccattaat caccaggaga tatggcgcac cagccagtta
     6361 gttagccaga tttgtaatat gccgatcccg gcaaacaaag ccattgttgg cagcagcgca
     6421 ttcgctcact cctccggtat ccaccaggat ggcgtgctga aaaaccgcga aaactacgaa
     6481 atcatgacac cagaatctat tggtctgaac caaatccagc tgaatctgac ctctcgttcg
     6541 gggcgtgcgg cggtgaaaca tcgcatggat gagatggggt ataaagaaag tgaatataat
     6601 ttagacaatt tgtacgatgc tttcctgaag ctggcggaca aaaaaggtca ggtgtttgat
     6661 tacgatctgg aggcgctggc cttcatcggt aagcagcaag aagagccgga gcatttccgt
     6721 ctggattact tcagcgtgca gtctggctct aacgatatcg ccaccgccgc cgtcaaactg
     6781 gcctgtggcg aagaagtcaa agcagaagcc gccaacggta acggtccggt cgatgccgtc
     6841 tatcaggcaa ttaaccgcat cactgactat aacgtcgaac tggtgaaata cagcctgacc
     6901 gccaaaggtc acggtaaaga tgcgctgggt caggtggata ttgtcgccaa ctacaacggt
     6961 cgccgcttcc acggcgtcgg cctggccacc gatattgtcg agtcctccgc caaagccatg
     7021 gtgcacgtac tgaacaatat ctggcgtgcc gcagaagtcg aaaaagagtt gcaacgcaaa
     7081 gctcaacaca acgaaaacaa caaggaaacc gtgtgatgtc gaagaattac catattgccg
     7141 tattgccggg ggacggtatt ggtccggaag tgatgaccca ggcgctgaaa gtgctgaatg
     7201 ccgtgcgcaa ccgctttgcg atgcgcatca ccaccagcca ttacgatgta ggcggcgcag
     7261 ccattgataa ccacgggcaa ccactgccgc ctgcgacggt tgaaggttgt gagcaagccg
     7321 atgccgtgct gtttggctcg gtaggcggcc cgaagtggga acatttacca ccagaccagc
     7381 aaccagaacg cggcgcgctg ctgcctctgc gtaagcgctt caaattattc agcaacctgc
     7441 gcccggcaaa actgtatcag gggctggaag cattctgtcc gctgcgtgca gacattgccg
     7501 ctaacggctt cgacatcctg tgcgtgcgcg aactgaccgg cggcatctat ttcggtcagc
     7561 caaaaggccg cgaaggtagc ggacaatatg aaaaagcgtt tgataccgag gtgtatcacc
     7621 gttttgagat cgagcgtatt gcccgcatcg cgtttgaatc tgctcgtaaa cgtcgccaca
     7681 aagtcacctc aatcgacaaa gctaacgtgc tgcaatcctc tattttatgg cgtgaaatcg
     7741 tcaacgagat cgccacggaa tacccggatg tcgaactggc gcatatgtac atcgacaacg
     7801 ccaccatgca gctgattaaa gatccatcac agtttgacgt tctgctgtgc tccaacctgt
     7861 ttggcgacat tctgtctgac gagtgcgcaa tgatcactgg ctcgatgggg atgttgcctt
     7921 ccgccagcct gaacgagcaa ggttttggtc tgtatgaacc ggcaggcggc tcagcaccag
     7981 atatcgcagg caaaaacatc gccaacccga ttgcgcaaat tctgtcgctg gcactgctgc
     8041 tgcgctacag cctggatgcc gatgatgcgg cttgcgccat tgaacgcgcc attaaccgcg
     8101 cattagaaga aggcattcgc accggggatt tagcccgtgg cgctgccgcc gttagtaccg
     8161 atgaaatggg cgatatcatt gcccgctatg tggcagaagg ggtgtaatca tggctaaaac
     8221 gttatacgaa aaattattcg acgctcacgt agtgtacgaa gccgaaaacg aaaccccgct
     8281 gttatatatc gaccgccacc tggtgcatga agtgacctca ccgcaggcgt tcgatggtct
     8341 gcgcgcccac ggtcgcccgg tacgtcagcc gggcaaaacc ttcgccacta tggatcacaa
     8401 cgtctctacc cagacgaaag acattaatgc ctgtggtgaa atggcgcgca tccagatgca
     8461 ggagctgatt aaaaactgca aagaatttgg cgtcgaactg tatgacctga atcacccgta
     8521 tcaggggatc gtccacgtaa tggggccgga acagggcgtc accttgccgg ggatgaccat
     8581 tgtctgcggc gactcgcata ccgccaccca cggcgcgttt ggcgcactgg cctttggtat
     8641 cggcacttcc gaagttgaac acgtactggc aacgcaaacc ctgaaacagg gccgcgcaaa
     8701 aaccatgaaa attgaagtcc agggcaaagc cgcgccgggc attaccgcaa aagatatcgt
     8761 gctggcaatt atcggtaaaa ccggtagcgc aggcggcacc gggcatgtgg tggagttttg
     8821 cggcgaagca atccgtgatt taagcatgga aggtcgtatg accctgtgca atatggcaat
     8881 cgaaatgggc gcaaaagccg gtctggttgc accggacgaa accaccttta actatgtcaa
     8941 aggccgtttg catgcgccga aaggcaaaga tttcgacgac gccgttgcct actggaaaac
     9001 cctgcaaacc gacgaaggcg caactttcga taccgttgtc actctgcaag cagaagaaat
     9061 ttcaccgcag gtcacctggg gcaccaatcc aggccaggtg atttccgtga acgacaatat
     9121 tcccgatccg gcttcgtttg ccgatccggt taaacgcgcg tcggcagaaa aagcgctggc
     9181 ctatatgggg ctgaaaccgg gcattccgct gaccgaagtg gctatcgaca aagtgtttat
     9241 cggttcctgt accaactcgc gcattgaaga tttacgcgcg gcagcggaga tcgccaaagg
     9301 gcgaaaagtc gcgccaggcg tgcaggcact ggtggttccc ggctctggcc cggtaaaagc
     9361 ccaggcggaa gcggaaggtc tggataaaat ctttattgaa gccggttttg aatggcgctt
     9421 gcctggctgc tcaatgtgtc tggcgatgaa caacgaccgt ctgaatccgg gcgaacgttg
     9481 tgcatccacc agcaaccgta actttgaagg ccgccagggg cgcggcgggc gcacgcatct
     9541 ggtcagcccg gcaatggctg ccgctgctgc tgtgaccgga catttcgccg acattcgcaa
     9601 cattaaataa ggagcacacc atggcagaga aatttatcaa acacacaggc ctggtggttc
     9661 cgctggatgc cgccaatgtc gataccgatg caatcatccc gaaacagttt ttgcagaaag
     9721 tgacccgtac gggttttggc gcgcatctgt ttaacgactg gcgttttctc gatgaaaaag
     9781 gccaacagcc aaacccggac ttcgtgctga acttcccgca gtatcagggc gcttccattt
     9841 tgctggcacg agaaaacttc ggctgtggct cctcgcgtga gcacgcgccc tgggcattga
     9901 ccgactacgg ctttaaagtg gtgattgcgc cgagttttgc tgacatcttc tacggcaata
     9961 gctttaacaa ccagctgctg ccggtgaaat taagcgatgc agaagtggac gaactgtttg
    10021 cgctggtgaa agccaatccg gggatccatt tcgacgtgga tctggaagcg caagaggtga
    10081 aagcgggaga gaaaacctat cgctttacca tcgatgcctt ccgccgccac tgcatgatga
    10141 acggtctgga cagtattggg cttaccttgc agcacgacga cgccattgcc gcttatgaag
    10201 caaaacaacc tgcgtttatg aattaatccc cttgcccggt caaatgaccg ggctttccgc
    10261 tatcgtccac gtcatcaaac gtctttaacc tttgcggtta aaaataatgc gacaacagaa
    10321 ataaccgcaa ttacccagta gac
//
LOCUS       DAESHI010000143        10141 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.145, whole genome shotgun sequence.
ACCESSION   DAESHI010000143 DAESHI010000000
VERSION     DAESHI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10141
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.145"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(1..1222)
                     /locus_tag="KW011_003731"
                     /pseudo
     CDS             complement(1..1222)
                     /locus_tag="KW011_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304967.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            complement(1985..2188)
                     /locus_tag="KW011_003732"
     CDS             complement(1985..2188)
                     /locus_tag="KW011_003732"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661971.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGLIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAV"
     gene            complement(2532..3212)
                     /locus_tag="KW011_003733"
     CDS             complement(2532..3212)
                     /locus_tag="KW011_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBH7661972.1"
                     /translation="MATTNAFSLASPPLARICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SMQMPGFRRQMNEGWYQIRIAGYDTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGL
                     QIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKA
                     KTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGK
                     VVVIGRQG"
     gene            complement(3110..3853)
                     /locus_tag="KW011_003734"
     CDS             complement(3110..3853)
                     /locus_tag="KW011_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH7661973.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLAAASACM"
     gene            complement(3864..4562)
                     /locus_tag="KW011_003735"
     CDS             complement(3864..4562)
                     /locus_tag="KW011_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBH7661974.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            complement(4562..4891)
                     /locus_tag="KW011_003736"
     CDS             complement(4562..4891)
                     /locus_tag="KW011_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH7661975.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(4888..7461)
                     /locus_tag="KW011_003737"
     CDS             complement(4888..7461)
                     /locus_tag="KW011_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBH7661976.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLADAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTSSFRANLQGAWDDRENARLGLAAATLQSDMEKAGELAARD
                     RAERESSQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILQADYNTLMASAK
                     KDYESTQKKPSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANEKISQQRRDLWK
                     AENQYVVLKEAATKRQLSEQEKSLLAHEKETLEYKRQLAELGDKIEHQKRLNELAQQA
                     ARFEQQQSAKQAAISAKARGLTDRQAQRESEEQRLREVYGDNPAALAKATSALKNTWS
                     AEEQLRGSWMAGMKSGWGEWAESATDSFSQVKNAATQTFDGIAQNMAAMLIGSEQNWR
                     GFTRSVLSMLTEIFLKQAMVGIVGSIGSAIGGAFGGGASASTGTAIQAAAANFHFATG
                     GFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMR
                     RAEGINFNQNNHVVIQNDGPNGRAGPQLMKAVYEMARKGAQDELRLQLRDGGMLSGSG
                     G"
     gene            complement(7442..7855)
                     /locus_tag="KW011_003738"
     CDS             complement(7442..7855)
                     /locus_tag="KW011_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBH7661977.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            complement(7882..8313)
                     /locus_tag="KW011_003739"
     CDS             complement(7882..8313)
                     /locus_tag="KW011_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBH7661978.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            complement(8327..9079)
                     /locus_tag="KW011_003740"
     CDS             complement(8327..9079)
                     /locus_tag="KW011_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH7661979.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKADAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWSATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VSAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            complement(9087..9482)
                     /locus_tag="KW011_003741"
     CDS             complement(9087..9482)
                     /locus_tag="KW011_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH7661980.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMTAIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(9479..10054)
                     /locus_tag="KW011_003742"
     CDS             complement(9479..10054)
                     /locus_tag="KW011_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH7661981.1"
                     /translation="MAIKGLDQAIDNLNRVRKNAIPAASAMTINRVATTAINQSSSQV
                     ARETRVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPQARYEEGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVDRIRRERLPKELAYALKQQLRIAIKR"
     gene            complement(10069..>10141)
                     /locus_tag="KW011_003743"
     CDS             complement(10069..>10141)
                     /locus_tag="KW011_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH7661982.1"
                     /translation="PDDGGSCYLWLNRGQPPAVNRRR"
BASE COUNT         2031 a         3278 c         2512 g         2320 t
ORIGIN      
        1 tcataaacgg caagatgcgg cgttgcggtt atctgaaaat agcccggcgt cagctcaatc
       61 cgcgacggtg ctgccggtgc ggcaatccgg aaggtggtgg tggcaggttc accctgctgg
      121 ccatagctgt ttatcgcccg caccgtcagg gtgtattccc cgagcggcag gccgctgaaa
      181 cggtgctccg tgtctgcggt gatggcggtg gtcaccagtc tggcatccgt tcccttacca
      241 ctggtcaggc gcagactgaa gcgcacaccc ttcaccaccc gcggcgtgtc ccatttcgcc
      301 tgcgccagat actggccgtc aggtgctgca ctgccggtgg gatgacgctg ttcagggaac
      361 ctgactgcgg ctcaaagcgg gcaccgttat ccacgatggc ttctttttcc agtacgtgct
      421 gcaccgccgt gatggcaaag gtgccgtccg tgttttcccg gacggagaca cagcggaaca
      481 ggagacggcg cagtgacggc agggagagtc cccacacccc gtatgtctcc acaccatcag
      541 gcagggtact gacctgtatc cggtccggcg cggggtgttc ggtgatgtcc acactcaccg
      601 gcttaccgct gccgttaatc aggttcaccg tggcggcacc ggtctccgga agtgtcactt
      661 cacggtccag cgtcagagtg cgggtggcag catcaatgga caggacacgt ccgccggtca
      721 gggtcccggc atagtcgtta tcacagattt caatgatgtc accgggtgtg tgccgcagcc
      781 cctgagaccc gagcgtgaaa tccaccgtct gcgtttccag cagttcggtc tttatcaccc
      841 acagtccggc acggtgggcc tgaccgcggc tggtacagcc gaacgcgtcc atcttcagca
      901 ggttgcgtcc gtagcgcagt atggcttccg ggtcttccac cagttccgtg gaggtctgcc
      961 agccgttctg cgggtcggta taattcacct ccaccgccgt gtgccggtcc ttcagggcac
     1021 tgaagctgta gcggaaaccc acgccgttat catccaccac cacatcgctg ttggtgtacg
     1081 gccacaccac atccgacggg cggtcctgaa caaacgtcag cgtctggccg ttccataccg
     1141 gcatacagcg catcgccgag cagaaatcac tgagcacgtc ccatgcctta cgctgttgtg
     1201 ccaggtacgc attgaaggtc atccgcggct cggtctcccc gaaactatcc gggaccgtct
     1261 ggtcgcagta ctgcccgatg gcatacagcg cccacttgtc cacatccgcc gcccccagac
     1321 gttttcccat gccgtagcgc gggtgagtca gcatgtccca cagacaccag gccggattgt
     1381 tgctgtatgc cggtttcagg ctaccgtccc agataccact gtacgtgcgt ttttccgggt
     1441 catagtttga cggcacctgg atgatgcgac cgcggatatg gtagttcacc gtcatctgct
     1501 ggccgccgaa ctgctccgca tccacctgca gccccacaat ggccgtgttc gggtagcact
     1561 gtttcacatc gatgatttcg gtgtatgacg accacagcgt cttattctgc agctggtccg
     1621 tggtgctgtc cgctgtctcc cggaccatcc ggatgttaaa aggacgggga ggcagattat
     1681 ccagaatcac cgacgccaga aactgcgagg tggtcttgcc gttaatggtg acatcctttt
     1741 ctgtcaccca gttaccgtta cgctgtaact gaatcagcag gcggacggat gtcgggtttc
     1801 ggtcaccctt tgacgtggtc tgcaccagtg actgcacccc gaaggtaacc cgcaggcggt
     1861 caatgttcgc ggacgtaatg gtgcgcgtca ccggttttgc cttcgtcact tccacgccca
     1921 gtccggtttc agcaccggag gactcaaagc cttccggtgg tgtctgctcc tgctccccgg
     1981 cgcgtcagac cgccgtcaca ccgtgtatca cgggattacc gtccgtgtcc gtcagcgggg
     2041 ttttgttcac caggatactc tgcagtccct tcaccggacc ttctatcagt ccctcaccaa
     2101 tcgcatcaat cacgctcatc atctgcgtgg atttgagatt atccttcgcc tcacgaggcg
     2161 tgtgtgcctt accgccacct tttcccatac agccttcccc tgaataaatt aaccgccact
     2221 tgccattccg tacagaagtc ggatatcctt cgcccgaaaa gcatgaaaca catttctgcc
     2281 atgctaaaga gaaaccccgg tatcagcaga taccggggtt ttctttcatg cccaccgata
     2341 atcctgttgg ttaaaaccgg taatggcata aaaattctga atatcttcac attttcacaa
     2401 actgactgtg gcgcgtataa tttctctgcg ttaatttttt tgtcgtgata taagaataat
     2461 tccttacact taatcttcgt aactctcccg cagttcctgt ccgcgatcac tgcgggattt
     2521 ttttattctt tttacccctg ccgcccgata accacgacct ttccgccccc gccttcatca
     2581 cgggtgctga tgtcctggga tatacggcgg gagccaacca gcatttcccc gtaaggcacc
     2641 ggcatcgggt tcccctgggc aatcatgtta tccagtgagg aaaagtacgt gttctgtctg
     2701 ccgttatccg ttgcgcggta atccggtgtt tttgccttcg gggccagcat ctgggccaca
     2761 ccgcccagaa tcatgctggc tccaagtgaa aacagcatcg tggtggcaga aaaaccaccg
     2821 gctgccaggg ctgaacccca taacgccatt gatgccccgg cagtgaagaa agagcccacg
     2881 atggctgccg cccccagcac aatctgcagt ccaccctttc cggccccggc cagtcgcggc
     2941 acaatgtgga tgaccgttcc ctcacccagc tgttcgtgaa gacgggcgta caccgcctcc
     3001 ggtgccgtgt cataaccggc aatacgtatc tggtaccagc cttcgttcat ctgacggcga
     3061 aagcccggca tctgcatcga cagggcgcga atggcttccg ctgccgtgtt cacatacagg
     3121 ctgaggcggc ggccaaatcg ttgtaaatcc ccgtgaaggc agatgcgtgc cagtggcggt
     3181 gacgccagac tgaatgcgtt cgtcgttgcc atttttcgga atacctctcc cgtttactca
     3241 gttgttcagg cagatggtga agcagctcac cgttgccgca gtaaatggcg gcatggttcg
     3301 gtaccgaagc accaaagcag cacagcagaa tatcgcccgc ctgtgcagag gacaggggca
     3361 cccggtaaaa gccggtgacc gccatattgt ccaggtaaag gttctgaccg ttgcgccacc
     3421 agtcatcctc gcgatgaaaa tccggcattt cagtccccgc cagatgataa gcatcccgga
     3481 acagcgtgta acagtccgtc accccgtgct caaagcgccg tcctgtcaga tgtggcacac
     3541 agcggaattt gtgaatgtca ccccggcaga ccaaccacca gggcagtgcg ctttttatct
     3601 gcaaccgccg gtcagcctcg ctcagccagg gcagcccacc gggatgactg tggaccagtg
     3661 ccacaatctc cccctgcatc tctgcccgca gccagtcttc cggtgcgata cgaaaatacg
     3721 cctccggctc tgcggaaata ttcacacaag ggatataccg ctccccctcc ggcgtgctta
     3781 tcacgaagcc gcacgactcc gcaggcgcac accgccgggc atgtgccaga atcgctgatt
     3841 cagtctgtgt cataaaccgg gatttactgc gaaagtttat taatggaaag gaaaccgcca
     3901 aaattgccga cattcctgcg cagttcacac ccgcgcatgc acttgctgca tctgtcctta
     3961 cggatatccg tggtggggtt gtcgaactca tccgccaccg caggaccgtt atacccgcat
     4021 tcatctcccc ggtaatccca catacaggtg ttcgccagca tgatgcgacc gggaaacagc
     4081 gccccgtccg tctcggtcgg tgtagccagc acaaacgagg ccgtcatggc tgtcagctgc
     4141 gacatctgct ccaccaccca gcggtcactc agctcctgct ccgggtccgc ctccggattg
     4201 cccgcaacga aattcaccgc atccagaaaa cgggcataca cccggcggcg gaccaccgtg
     4261 gcccccacca gactctgcag gtcttccgcc atcccggtga ccagaccgaa cagattggac
     4321 accgtcagcg acggtctggc actgctgccc cggccgttca tctcaaagcc gctgccgtca
     4381 atcgggtatg cctcatactt acgcccctgc caggtgaccg gctccccttt ttcattcagc
     4441 tcattacaga aaaaataccg ctcaccaccc tgtaccgtca ggtcgatttc ccagagtacc
     4501 acccgcggtg actgctctga cttaaccgac tcgttcagac tttcttcgcg aatatcctgc
     4561 atcagttcac cacctgctta aactccgcgc tgaactcaac gcgcaacatc ccgacccgcg
     4621 cagaccaccc ggcacaggtc acctttatct gccggtatgc atagggtggc ttccacaaaa
     4681 atgccttcca gccaccgtgc tctgccagga acgcttccag atgccgggcc tcctcccggg
     4741 tcacggaaag cgtcaccctg tatgttttca ggtcagcatt cagccctgcc gccatacgct
     4801 gtgagtaccc gtcaccaaaa cgcacttcac gcaccgatgg ctgcgagttc acctccatat
     4861 ccggcttcac tttccagcga aaggttttca tccaccgctc cctgataaca taccgccatc
     4921 acgcaactgc agccggagtt catcctgtgc ccccttgcgg gccatctcat acaccgcttt
     4981 catcagctgc ggccctgccc gcccgttggg gccgtcgttc tgaatcacca cgtgattgtt
     5041 ctgattaaaa ttaatgcctt ccgcccgccg catctgcgcc ggacttccgg caccgccgac
     5101 ataaccacct tccgcatagc cccgcatcag gcggtacaga ttgccgacac caatccggct
     5161 ggtcgcctcc ttcgtgaaga caaactcccc gcgatgaaca atccccgcag gttcatattt
     5221 accccccgtc cccgtaaatc ccccggtcgc gaaatggaag ttcgccgccg cagcctgaat
     5281 ggccgtcccc gtggaggcag acgcaccacc accgaaagca ccgccaatgg cgctgccgat
     5341 actcccgact atccccacca tcgcctgctt cagaaaaatc tctgtcagca tggacagcac
     5401 agaacgggtg aaaccacgcc agttctgttc gctgccgatc agcatcgctg ccatattctg
     5461 tgcaataccg tcaaaggtct gcgtggccgc gtttttaacc tgcgaaaaac tgtccgtcgc
     5521 actttccgcc cactcgcccc agccggactt catcccggcc atccagcttc cacgaagctg
     5581 ctcctccgca gaccaggtgt tcttcagtgc agatgtggcc ttcgccagcg cagccggatt
     5641 atcaccgtac acctcacgaa ggcgctgctc ttccgactcc cgctgcgcct gacggtcggt
     5701 gagtccgcgg gcttttgcgc tgattgccgc ctgcttcgcg ctctgctgct gttcaaaccg
     5761 cgccgcctgc tgtgccagct cattcagccg tttctggtgt tcaatcttgt cgcccagctc
     5821 agccagctgg cgtttgtact ccagcgtttc tttctcatgg gccagcaggg atttttcctg
     5881 ctcagataac tgccgtttcg tggcggcctc tttcaggacc acatactgat tttccgcttt
     5941 ccataaatca cggcgctgct ggctgatttt ttcattcgca ccgctgtgct tctccagcgt
     6001 cctgagctca gtttcaagcg ccagcagggc tgcatgcgcc tggtcttcct ggcgctcacc
     6061 ggcagacacc ttcacaccgg acggcttttt ctgcgtcgac tcataatcct tttttgccga
     6121 cgccatcagc gtgttgtaat ccgcctgcag gattttcccg tctttcaggg ccttattcag
     6181 ctcttcctgc cgggcggtat atttctccag tggcgtcagc aggcgctcat acgccttctg
     6241 cgcctctccg gtatacttca gctgtgacga ctcacgctca gccctgtccc ttgccgccag
     6301 ttcaccggct ttttccatat ccgactgcag cgttgccgct gccagaccca gacgggcatt
     6361 ttcccggtca tcccatgcgc cctgaaggtt ggcccggaaa gaggaggttt taccgcggcg
     6421 ctggctccga ctctggtacc actgccattt tttatccgcc tcatcaaatg ccttctgcgc
     6481 actggcgagc atatccgctg aggattccgg acgaccgata tccagaatgg catcccacat
     6541 cgatttgaat gccttccctg ttttatccgc ccaggtctcc agtgttccca tgttttcttt
     6601 caggcgacgg gtctgctcat caaagccttt cgtggcgata tcgttcgccg cctgcaatgc
     6661 cccggcctcg tctccggaac gctgcagctg tgcaacatac gcaatctgct ctgccgtcac
     6721 gttacggaac tggcgcgcca tcgccatcag tcccgacgtc gggtcagtgg tcagcttccc
     6781 gaaggcttca gcgactttat ccacctccac accggatgca gacgcaaaac gcgcgacact
     6841 ctggttgatg gcatcaaact gttcaccacc acgcacaccg gcattcacca gggctgccag
     6901 tgactctctc gcctggttaa acgtcagccc tgctgcctgc ccggctcttg agagagtcag
     6961 catacgatcg gcagtcagtc cggactgatt accggaaaga accagggttt tattaaacgc
     7021 tgaaagcgtg gaatctccct ggtaccaggc gtacaccagt gcacctgtcg ccaccgccag
     7081 cgaggtgacc ccgaccatcg gcagggtgat cgcatcggca agccccctga acatggggat
     7141 catcccgccg aaggagtcct tcacctgacc gccctgttgc agcaggatca gccagggatt
     7201 ctgaccaccg gcaagctgcg tggcgatatc cgtaaactgt gcgggcaggg ttcgcatggc
     7261 cgctttatac tgcccgacgg aaatcccggc tttttgtgca gccagcgcct ggcggctcag
     7321 gccctgttca acagcactgg cggtttttct ggcgtcggta tccagacctg aaaaatgacg
     7381 ccttacccgg ctcatctgct catcgaaacg gacagcatcc agactcaggt caataacaag
     7441 atcaccaacc ggctgggaca tatctcacac ctcccggaat ccccgctgaa gccatcatta
     7501 atgcggcatc atccaccatg acatccgcca catccgcaga cgataaaata tcgcgccctc
     7561 cgtccccacc gaaccggacg cctccggcaa gtcctgccgc tttctgcatc agcattttgt
     7621 cctcatccgg cctctccacc tgctcttcct catgccgggg gacaagcaga ctgaaatcag
     7681 agggatgcat atccggatcg caaaaaaaca ggctgagtac agcgtacgtc agcccggaaa
     7741 aatgcatatc cagctgggta tcctgaaaat aatgcgtgcg gtaaaaacgg cgccagtcgg
     7801 catattcggt ggatgtcatc ccggcaagca tggcgcgcca gtcgggtctc cccatctcac
     7861 gcgccagtct gagggcaaag ttcagctcgc cgtcgaagac tttcccgcag aaaaatcatc
     7921 atcagtcagc gtgttatttt tcgccacttc agtaatatca gtatccggac gaacagcttc
     7981 gatcatcccg gacaggcaca acaccacgtc ttccgcccgg gcaatggcat cggcaggcca
     8041 ggtggtgagc acttcctgct ctatcttcat cacggcctca ttcattgacg gtgactgcgt
     8101 tttctgtgga tggttatgcc acagggacat cgccaccaga aacgcgccgg ttctgacgag
     8161 atcttccacg cttacctgca ggttgccgct ggattctgcc tgttctgcac gccgtttcag
     8221 gagggcaaga tgctcgatac gctgcagcgc agacaattcg gaaagcgtga cagacacacc
     8281 gttatattca aattgttctg ttttcagaaa catgtattac ctccgtttac cctgcagcgc
     8341 ccgcttcagt aacggtgact tcagccactg cggcgaactg accatttccg ctcaccacag
     8401 ggatctgcac cttacctgtc gccacgccgt ttaccgtaat tgtcatatct ttcacactaa
     8461 tggtggcttt cgacggatcg gcggaaaccg ctctgaacgt cttgtcggtt gcactttccg
     8521 gctcaaaaga aaccgtcagg gtggttgttt tcccttttgc caccgtaccg gatgtcggcg
     8581 tcaccttaat cgcactgacc ggcgtaattt tgctgcgttc ttccgctaca gaaggtttac
     8641 ccacgttagt gactttcacc gtgcgggtga tcacttcttt cgccgtcacg gccttaccga
     8701 tactgctgac ccagccacga aacacatcca ccgtgccatt cggaaaacgg attttatagg
     8761 cccggacatc gccgctttca aaccagccta taagcccttt ctggccttcc tctcccggtt
     8821 tccaggccag cgtaaaactg gtatcacctg cagatttctg cccctgcccg gtcgcgctcc
     8881 agtccgcgtc ttcatcatcc aggtagttat catcatagga ttcagccgtc atctcgcccg
     8941 gcgtcagatc cttcacctta gccagtcgct gccagtcatc gtctgacaac gggtttgcat
     9001 aagcatcagc cttgccgttg taaacccaca gagtggtacc ggcacctttt accggctcca
     9061 ggggatttgg tgttgccata tcgtcctcac atctcgtatg taatggaata agtcagatcc
     9121 gcagagctcc ataacgccat atcgtcatca cgacgatact catagccctg cgtaaccatc
     9181 gtggtaatca gtcctgccag tgcagggatc gcagtcatcg ccggataaat ccggctttcc
     9241 atccactgat cgagctccga atcaggtacc tgtgccggta aaaacacctc aatatgcagt
     9301 gtggcccgcc aggtatctgc atccagctct tcaccggtat actctgcatc cgtcagataa
     9361 accgcgatcg caggaaaatc ctcttcgtca aaaacaacgg ggcgaccatc aaacagcgtc
     9421 gccccgtgtt catgctgctc gagtgcatcc agcactgcgg cacgaatgtc agtgtgtttc
     9481 atcgttttat cgcaatcctc agttgttgtt tcagcgcgta tgccagttct ttaggcaggc
     9541 gttcacgccg gatacggtca acattctcat caaatgcctg tttcagtggg gccgccatcg
     9601 ggattttcac cacctgaatg ggaaggcgat tacgcttttt ccttcccttg tcgtcattgc
     9661 cctcctcata tctggcctgg ggaagacgtt gcataacatg ccagcgccca ttatttaatc
     9721 gctggataaa tgcccgctga taacgatgct gaccggcttt gagtatgctg ttcggacgac
     9781 gccccagcat tctgatcccc agcttaatca cagggagatc accgcggtta acgataattc
     9841 tggcattcgg atttctgacc gtcgcccgtt tcagtctgga ccgttccttt accagtttcc
     9901 gtctcaccct ggtttcccgg gcaacctgtg acgaagactg attaatcgcc gttgtggcca
     9961 cgcggttaat ggtcattgca gaagcagccg gaatggcgtt tttacgaacc cggttcagat
    10021 tgtcaatcgc ctgatcaagc ccttttatcg ccataattca ccctgcgttt atcgtcgccg
    10081 gttaactgcg ggtggttgcc cacggttgag ccagagataa cagctgcccc cgtcatccgg
    10141 a
//
LOCUS       DAESHI010000144         9785 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.146, whole genome shotgun sequence.
ACCESSION   DAESHI010000144 DAESHI010000000
VERSION     DAESHI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9785
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.146"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(104..418)
                     /locus_tag="KW011_003744"
     CDS             complement(104..418)
                     /locus_tag="KW011_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HBH7661983.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
     gene            complement(423..638)
                     /locus_tag="KW011_003745"
     CDS             complement(423..638)
                     /locus_tag="KW011_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBH7661984.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(789..2642)
                     /locus_tag="KW011_003746"
     CDS             complement(789..2642)
                     /locus_tag="KW011_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HBH7661985.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCCGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
     gene            complement(3618..3694)
                     /locus_tag="KW011_003747"
     tRNA            complement(3618..3694)
                     /locus_tag="KW011_003747"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3658..3660),aa:Arg,seq:tct)
     gene            complement(3708..3784)
                     /locus_tag="KW011_003748"
     tRNA            complement(3708..3784)
                     /locus_tag="KW011_003748"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3748..3750),aa:Arg,seq:tcg)
     gene            complement(3792..3867)
                     /locus_tag="KW011_003749"
     tRNA            complement(3792..3867)
                     /locus_tag="KW011_003749"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3832..3834),aa:Ile,seq:cat)
     gene            complement(3908..4966)
                     /locus_tag="KW011_003750"
     CDS             complement(3908..4966)
                     /locus_tag="KW011_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HBH7661986.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(5074..5314)
                     /locus_tag="KW011_003751"
                     /pseudo
     CDS             complement(5074..5314)
                     /locus_tag="KW011_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5539..6360)
                     /locus_tag="KW011_003752"
     CDS             complement(5539..6360)
                     /locus_tag="KW011_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBH7661987.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(6357..6737)
                     /locus_tag="KW011_003753"
     CDS             complement(6357..6737)
                     /locus_tag="KW011_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBH7661988.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            complement(6738..7724)
                     /locus_tag="KW011_003754"
     CDS             complement(6738..7724)
                     /locus_tag="KW011_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBH7661989.1"
                     /translation="MSAFHNGRILVEPEPKSMRNLPSGVVPAARQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSVYSESDIIPGEQTATSILKQRTKNIALLPHVHQQQNPPQEKTVVS
                     IAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTK
                     ADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            complement(7798..8076)
                     /locus_tag="KW011_003755"
     CDS             complement(7798..8076)
                     /locus_tag="KW011_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661990.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            complement(8143..8403)
                     /locus_tag="KW011_003756"
     CDS             complement(8143..8403)
                     /locus_tag="KW011_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661991.1"
                     /translation="MKIEELREIFSEDGLYTVRVENGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFISRANEFVTSLPSLKEAGIKAGFSAFEDE"
     gene            complement(8624..8779)
                     /gene="hokD"
                     /locus_tag="KW011_003757"
     CDS             complement(8624..8779)
                     /gene="hokD"
                     /locus_tag="KW011_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBH7661992.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(9071..9304)
                     /locus_tag="KW011_003758"
     CDS             complement(9071..9304)
                     /locus_tag="KW011_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661993.1"
                     /translation="MDIAVLEIALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLT
                     WQLRKAKPDCKLPDKAMSYLERNGLISVEDILR"
     gene            complement(9412..9579)
                     /locus_tag="KW011_003759"
     CDS             complement(9412..9579)
                     /locus_tag="KW011_003759"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661994.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            complement(9590..>9785)
                     /locus_tag="KW011_003760"
     CDS             complement(9590..>9785)
                     /locus_tag="KW011_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7661995.1"
                     /translation="SPKKIGFYTIGDKSIYLYDLRRMDEIMEALDNRSSMDWCVAVHD
                     MNAGFDEKILFPSSVESTAG"
BASE COUNT         2312 a         2739 c         2235 g         2499 t
ORIGIN      
        1 cagcgccaga acagcagctg acaggccgta tttgattttt gcgctcatgg atatttatca
       61 ggatgctacc aatgaaagat actggaaagc caactgcaaa aagctaacaa cccgtaatcg
      121 agttatcaga actgttaatt tttatggtat accgcgcctc tgaacagggg cgcgtttctg
      181 gcaacagctc gtccccttca cataacccgg cagcaacatc caagaagacc tgtctgatgc
      241 tccttctggc tgctgcctca taaaactcca gcgcggcacc ttcaacacgg tccagcgaga
      301 tgtccaggtc aaaaatttca ccgtcaaagc gttttttgtc ccgtaacgct aaagttaccg
      361 taactttatt ctcaaaattg cggatccctt tcacaatcag ttcatagttt tgagtcattg
      421 aattactctc cccgtgcagc cttacgacgg tcctctctga ttttgaaata caggttagtc
      481 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
      541 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      601 tatgacacac ctgttgtgat tttttccatc tggtacatac cccgtctccc gttatccgga
      661 agctgacaac aataaaaaaa gccaccagtt aagtactgat ggctctgata actcatgcag
      721 gcatctcaga cgacccactg acactaccgg tgagtttaac gataccttcc atttggctgg
      781 ctcacttttt atgatgatgc cggtgcattt atctccagca ccagactttc tatctcaacg
      841 ccatacgctg catttttggt aatatccgtc agcgtcagcg cattcagccc cagtgtcaga
      901 ctgtctttta tgacctggaa tgccgggcca gccactccat tcagtttcgg agtaaccgtg
      961 gcactgccgg cggtgaacac cagctccagc gtcagccagt cgttactgta attcccgaac
     1021 tcgcccaact ttgtgtttcc tgctttcttg tgatgcatca gattcagttt gccgtctgtg
     1081 gtctgggtga agaacgacat caggaacggg ttaccagtcc cggtcatcgc cacgacgtca
     1141 ggtaacgcta catcggtata cagataaatt cccagaccga actggttgtt ggtcagtgcg
     1201 cctgacagtc gaaacttaca gctcagtctg ccaccccgtg tcagcaggga gactgcgtca
     1261 tccaccgggc gcgtcaggga ccaggcttta ttgctctgct tggtgatctt aaatacacca
     1321 tctgacaact gaactccgcc attcttaatg ctccagccct gcgcagcagc gtctccggct
     1381 gtcggcaaca gggagattgt gcgtatggat gcatcttcag acggccccga tggcgtgtcg
     1441 ccgccgggcg agggtttgat ttccggtgcc ttaccactaa tgaaggctaa ggtgcgaccg
     1501 gctacgttca gaatagcagt tgccatacga tcgggaataa tgccacgacg cgcccatgag
     1561 ctgaaatgcg tcgggcgatt tgatgatacc cagtttttgt tcgttcggga tgccgaaccg
     1621 taataaccag acccggcaat atcaggatct tctgacgggt tgtttgtcgg tgtattaact
     1681 ccgctaccat cggtcataaa gggaacaaaa taaatctgct gggattcttt acctttatat
     1741 gcaccatata ccacttcata ttgcgtaccg tgttcttgtt tccacgcgta tgtcgtgtcg
     1801 ccacaaatcc aggggactga tgccggactt ccaccgtgac actgcgccgc cagcccggca
     1861 aggtcagcac ggaactgctg taccattgca agaaatgctg ctggctgctg ggcgtaactg
     1921 gcattcgtca tatcgaattc cccctgcatc cagcatatcg ccagcaaaac gtttttcggg
     1981 tttttctgca atgctgcctt cgtgcggaaa agcagatcct gatataacgg cttacccact
     2041 ccccagcgag ccgaatcctg actggccccc gtggactcgc tgaatgtccc ctccgtgccc
     2101 tgggtgaatg ccgaaccacc acaacagcat ggtaccagca ggatccccgc attattaggg
     2161 atatacggaa gcagtttttt ggcaatatgt aagccctgtc cgacacagcc gtactgccct
     2221 ttgctcaggt cagcccgggg atggttaatc gtactcatat cctgaacatc atgcagacaa
     2281 tggtcagcag gaatgatgtc gttaaatacg catacttcac caccgggagt cactgtgtta
     2341 cgacgggcca gttgcttaat gcgcggatgg ggcgcatcgt atgaatccgg aagcggaaga
     2401 ccttcaccgt aagccatggc attggattgc ccggccagta cgatgacgta gtaccactcc
     2461 ggctcagttg caccactgac gaccacatca ccttctgctg caatcgcctg catcagggta
     2521 taaggggtta tggccaccgg actaccaaac ggctgccagc cctccttcag tttttgtgtc
     2581 agtcgtttcg caaggtctga cggcgatgcc gccctgacca cgtcatagtg tttaaatgcc
     2641 atgaatcctc ccggccggga taatattgtg agtaaaataa ggagcgggct gaagtccgga
     2701 agttacagga caatggcaga agagagacga cagcccgcaa ttcgaaaaag accgcgcagt
     2761 tgcgcagagt gattactatg gggtattatt cgccagctga aatattactt cacgttttat
     2821 tgtttattcc ttgccgcccg cgtctcccag cgcgggcttt ttttgcccac aagaaaaccc
     2881 ctccggagag gggctaaagc cgcgtatctg tatcatcatg cacatggtgc cgggtgcctc
     2941 ccggtgagtt cagcccggtg ccactaaacc cgcgtcattc tcgttttgat aatcagagat
     3001 tataccgtca ccagtcgccc ctccgctcag ggggattcac catgcgaaat ttttttaaca
     3061 aatgcccagt ctgacaggca actgtcaact tactgaattg tgagcaacat agcatttaac
     3121 ggggaacctg ttttctgcag taaaaaggcc caccggagcg gatgggcctg gaaggatagc
     3181 ggtcatgtga tgccggtttc ccggtaactc agcaccggta tctgagtcaa cgttttctct
     3241 actgggtcat ttccgatacg ccctgcctgc tgacaggctt tcatcacatc tgaaaatata
     3301 gcaccctgac tgatactgta gtacctaagg ttccagaaac tgtgatgtat ccggcacaga
     3361 aaagcccctc cggagagggc tggagagtgg cgctatgtgc cattgcatga tgccgggtgc
     3421 ctcccggtga attcagtacc agcacctgaa tccgcgatta tcccatatac ctactcgctg
     3481 attgcccctc cgcacagggg gattcaccat gccagtttct tttaacaaac tccccgcaaa
     3541 acagacaact gtcaaccgtc tgaattgtga gacatttaaa aaaaaggccc gcaaaagcga
     3601 gccagggaaa ataagtgtgg cgcgttgtac tggattcgaa ccagtgaccg attgcttaga
     3661 aggcaattgc tctgtccggc tgagctaaca acgcatgatg cagataatgg accgccatcg
     3721 gggacttgaa ccccgcgcag ccagcttcga aggctggcgc tctttcctga tgagctaatg
     3781 gcggtatgtg atggtggccc ttgctggatt tgaaccagcg acctggcgat tatgagtcgc
     3841 tcgctctcac cactgagcta aagggccggg agccgcataa taacgacgcg taattaattc
     3901 ttcaatatca tccgttctgg ctgactaaat cctgtacttc ccgaaccgtc tgctcaaaac
     3961 gttcagtctc cagctcaacg ccaattgcac gacgcccgag cgccagtgcc gctttcactg
     4021 tcgaacccaa ccccatgaaa aaatctgcaa tcaggtcacc cggacgactg ctagcgctga
     4081 ttatctgctg cagcatttct gccggttttt cgcacggatg tttcccggga tagaactgca
     4141 ccggtttatg cgtccacaca tcggtatacg gcacctgcac cgtcacgcca aaataccgcc
     4201 gcagatgttt atattcactc agcagctccg catactgccg gttcagtgag gtatacgtat
     4261 ccaccagctg gtggtggagc ttttccagtt caccacgccg atgcttctct tctgccaccc
     4321 gggcaaacag cgcctgtaat ttcagataat cgctttcatt cggcaactgc cactgaccgg
     4381 cactgaacca gtgcgacacc atgtttttct ttcctgtggc atctgcaatc tgttttgccg
     4441 ttatccccag ggcagctcgc gcatcacgaa agtaagaaat cagcggggcc atcacatgct
     4501 gtttcagtgc actgcccttc gccgcatacc cggcatcttt cggacgatac ggcccctgat
     4561 aatgttccgc gaacagaatg cgctctgtgg cggggaaata cgcccgcagg ctttccttgt
     4621 tgcatccgtt ccagcgtccg gacggcttcg cccagataat atggttcagc acactgaagc
     4681 gttcacgcat catgatttcg atatcagatg ccaggcgatg accacagaac aggtaaagac
     4741 ttccggcagg tttcagcacc cgccagaact gcgccagaca ctggtccagc cacttcaggt
     4801 aatcatcgtc gcccttccac tggttatccc agccctcagg cttcacttta aagtacggcg
     4861 ggtccgtgac tatcaggtca acagaatttt cgggtaacga ccggataaat tccaggcagt
     4921 cggcgttgat taactcacaa ctggatattt ttacagtatt aagcatggat cattaagcct
     4981 gtctctgata ggctcattct gcttttgcgc aaagcagtgg gcctgaggtt tgcttgtgaa
     5041 cccaacgcat gagcagatgg ctggtgggtg cccctaacac ccaccagccg cccatttacc
     5101 acaaataaaa aagccttcac tgcggaaggc gtctgtaaca accgaactga tagtctgcca
     5161 gacccgccat aaccagctgg gtcagtatta actggcagcg ttcgcgtgaa aggtaagtat
     5221 tctgcgctat ctccccgact gtcgccggtt cggtaacgct taattcatta aacaccactc
     5281 tggcggtttc tgtcatatcc tgctgtttta gcatgtcttt tccttttccg gttaacgtga
     5341 cacaccaata actcttgtcg aaaaagccag caagctgaaa gacaggtatt caccgccacc
     5401 agcgcgttta ctgtactgac gcgatttcag tcataaaaaa cccgctcggc ggcgggttgt
     5461 agaaactctt ctaacgtcag gcataaaacg cccatcgtta tgacgaattt accacagatt
     5521 ccggaaaaat caaccttgtt acctagttac cttttttaac tgccgctcag cccatgcttc
     5581 ttcaatatca aaccgggtca ccagcgcatc ataaaatttc ttaactgttt tttcccatga
     5641 cgcgcgtgtt atctggtttg tcacctcgca tatagcatta aatgcctccg ttgatggtag
     5701 tctttcatag ccacgaccac cacaacgctg gcagtctctg ataacaggca taccacgttt
     5761 taccgactct tcacgatgaa tggcgacacc acgcccacgg caatccttac aggcggtgga
     5821 aacctcaccc tttccgccac actccggaca ggcaactttt accacctccc tgactttttt
     5881 ccattcttcc cagtaagacg gatacacacc tttcgtacac tttgcccata ccggcggctt
     5941 accatccgga tactggatct tgtttgtaaa aacctcgctt tcaataaatt tttttccgtg
     6001 acagcagggg cactgttttt tgctcgccgc gctacgggca taatcttcaa acgcatacga
     6061 agccataata cgcatcactg ccggttttat ttctgccggg agttttctta acgccgccac
     6121 gcgatcacac cgactgagtg catattctgt cagcaattct gttgcccgct ctctgtcatt
     6181 catactaatg cccattttcc caaggaacgc agaaaccccc atctcagccc gattctgtgt
     6241 catgccctgc gcggccatca catcagtgat actcagcgca tctttcgacg ttgaggccga
     6301 tgcatcagtc aggccggggg attttgggga gtagtatttc ggtaaatctt ccagtttcat
     6361 tttttgacct gcccttcaag cattatggga taaatcttca cccccagacg tccaccagat
     6421 actggctgag cacgaacgat attgatttca tcaaactgct catcgtccat tagcaacccc
     6481 gcatgcgtca gcgcatccag cggcgctttc agaatattgt ccaggtcacg gcggcgctta
     6541 tccggtggtt ctgcaataat ttttattgcc aaccttccgg acaggcttaa tttcagccgc
     6601 tgctggcgaa caataagcgc cactgcccgg cgataacgct ccccggcttt tgatacaaaa
     6661 tatgtgctgc cacgacgacg ccagtaagtg ttcaccgtcg gcgggtaagg taaaacaaat
     6721 tctatgcgct cggtcattta tgctttccac ttcagaacac ccgaatttct cgcgtgcatt
     6781 aaaaaacgaa tcagcaacaa cagctgactg ccgtgtttct cttcaaaatc ttttactccg
     6841 gcatgtagtt cgttacggca tttacggcat aacggaataa caaacaaatc atcagccttt
     6901 gttcccatcc ctcccagtcc atgaccaatg atgtgatgcg gatcatctgc ctgattgcca
     6961 cacgtcatgc atttctgcgt ttttacccag cgcgtgtata caggcatctc ttcccgttgt
     7021 gatttctggc gctggagata ctgagccggt gactccggat caacggcaat gctgaccacc
     7081 gtcttttcct gtggtgggtt ctgttgctgg tggacgtgag gcagtaacgc aatatttttt
     7141 gtgcgctgct tcagtatgct ggtggctgtc tgctctcccg gtatgatgtc gctttcactg
     7201 tacacagagc gaattttttc cgcacgcaac cccagcgaac gacgtaatac cgcttccggt
     7261 agcgcgtccg ccacctgatt gcggaccgcc caccaggata attcagccag agataattca
     7321 cgctcctgcg taccgcttat tgcgtgaccg atgacgtcaa tcatccatgc tgacaggttt
     7381 tgatgagcaa gttgctcgag tgattcggat gtctggtcac gcagctggtt gtcgcagtgc
     7441 cagcacaaca ccattgcgcc ggtaccataa cggtgaatga cggtttcgct gtgatgataa
     7501 tcgccgtgtg gccactggca ggatttaata tggcgcaaca gccagtcaga caatgcacca
     7561 gcaccaccag cagcacgaat cacccgtgcg ttactgaaaa acggcagcaa tgttttgtct
     7621 tccaccagcg gctggcgagc ggcaggaacg accccggacg gcagattacg catgcttttt
     7681 ggttccggct ccaccagtat tctgccgtta tggaatgctg acattgattc acggcctggc
     7741 ttaacgataa ccagaccgag ttccggtacc agaacaggtc gaagtaatac ccgcacatta
     7801 cctccagatc cgttgctgga atgtgcggga cggacgcgtt ggccgttcgg agtaagggag
     7861 cctgacggag attatccagt gacggtagtc gaggctaagg gcttttttaa cctcgcatcc
     7921 gcgcctgcgg taacactgaa tgagccattc ggcctgttct tcagtgcatg ggggatgctg
     7981 gtaccaatca gatttaaatg cgtgagagcg ccgcccgtgc ctgctggcaa agacggctga
     8041 attatcagaa ttgtgtagtc tggaattttg cgccatcggc tttctccggt ggcacagtgt
     8101 tactcaacag gggttcagcc ctgcgctgaa ttgtagatga attcactcat cttcaaaagc
     8161 agaaaaacca gccttaatcc cagcttcttt cagagacggc aacgatgtga caaattcatt
     8221 tgcacgcgaa ataaaaccat ccgtcacaag cccatccaca aaatgaatta acgcagctcc
     8281 actcttcctt tgttgagact gtaaacattt aatacggcag tggctgacaa tagcgccatt
     8341 ctcaacgcgc acagtataga ggccatcttc actaaaaatt tcacgtaatt cttcgatttt
     8401 catcaacaga atccttccag ataaatagca ctcccctgtt cggggtccat ccctcttctc
     8461 cctgcgcgct acttaagtat ttttgattct attccggcac catctaaaac ttcaaacgcg
     8521 ttgaaaataa aaacaaaaac ccgccgaagc gggttaagtg tgggtgcatt gaggatgcct
     8581 gacccatcag aggtggcgag ggatttctcc cccgcctggt ctcttactcc tcaggttcgt
     8641 aagctgtgaa gacagcgacc tccgtctggc cggttcgaat tcgtacctcg cagaggtctt
     8701 tcctcgttac cagtgccgtc actatgacgg ttaaacagat gacgatcagg gcgattaaca
     8761 tcgccttttg ctgcttcata gcctgcttct ccttgccttt cggcacgtaa gaggctaacc
     8821 tagatttgcc gttcatagat tgagcctcag attaatgtta agcgtcttgc cggacgcgta
     8881 atgttaactg gggcttttct ctatctgcct tttggtgttc atgcctgaga cagatagcct
     8941 caagcacccg ctgcaattct acttaactct ccttttcccg caaaccgttt ttatccccag
     9001 cggcaaatcg aatacaccac cagcgccacc gccatcgcaa ttcctaccgt tgttaatgct
     9061 tcaggccagg tcatcgtaaa atatcctcca cgcttatcag tccgttccgc tccagataac
     9121 tcatcgcctt atccggtaat ttgcagtctg gcttcgcttt tctcagttgc caggttaact
     9181 gctttaccag catggttaac tcatcgacca gacgctgata tcccactggt ttgtattcat
     9241 gcaatttacc ggctggctct gctgccagcg ataccagtgc gatttccaga acagcaatat
     9301 ccatcttata tgtgcggatg atgtcatggt cgattgtgcc cggtatgcac agtctctgtg
     9361 cttcaatagt ctcctctgcg tgagctatta actgctctct ggtaaaagtc gtcatgccgt
     9421 agccccttct tgatattttt caaaccagaa cacaaccggc tctgcttcca gcgatgccag
     9481 cgcaatccgt gccagttcca tttgttcacc acgggtaagc ccgttttcaa gcgggttttt
     9541 aatgaacaat tcaatacgtt ctttggtaat agtggtcatg tgttactcct taacccgcag
     9601 tgctttcaac tgatgagggg aacaaaatct tttcatcaaa ccctgcattc atatcatgaa
     9661 cagcaacaca ccaatccatc gacgaacgat tatcaagagc ctccatgatt tcatccatgc
     9721 ggcgtaggtc atacaggtaa atgcttttat cgccaatggt gtaaaagcca atttttttcg
     9781 gtgat
//
LOCUS       DAESHI010000145         9674 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.147, whole genome shotgun sequence.
ACCESSION   DAESHI010000145 DAESHI010000000
VERSION     DAESHI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9674
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.147"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..1710
                     /locus_tag="KW011_003761"
     CDS             <1..1710
                     /locus_tag="KW011_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HBH7661996.1"
                     /translation="EMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTM
                     TLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQ
                     STIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGD
                     ELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDAL
                     YLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNE
                     MGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGM
                     QVK"
     gene            1711..3360
                     /locus_tag="KW011_003762"
     CDS             1711..3360
                     /locus_tag="KW011_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HBH7661997.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPMQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3365..4141
                     /locus_tag="KW011_003763"
     CDS             3365..4141
                     /locus_tag="KW011_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HBH7661998.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="KW011_003764"
     CDS             complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="KW011_003764"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HBH7661999.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5430..6752)
                     /locus_tag="KW011_003765"
     CDS             complement(5430..6752)
                     /locus_tag="KW011_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HBH7662000.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="KW011_003766"
     CDS             complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="KW011_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HBH7662001.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="KW011_003767"
     CDS             complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="KW011_003767"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HBH7662002.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="KW011_003768"
     CDS             complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="KW011_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HBH7662003.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
BASE COUNT         2354 a         2484 c         2605 g         2231 t
ORIGIN      
        1 gagatgcgcc agacgctgac gctgcacaaa ggggcgaatt atatttcgct ggcgcagaac
       61 attcagcaat ctggcttgtt aagcgcagaa ctgcaacaaa atgggcaagt gcaggacagc
      121 attagcacaa aactgtcttt tgtggataac agctggcccg ttgaacagca gaaaaatgtc
      181 atgctcggcg gtggcgagaa cgcgctgatg ttgcccgagc aggctagcaa tatccggcta
      241 caaagtagcg aaacgccgca ggagattttc cgcaacaatc ttgatgcgtt agtcgatgga
      301 ccatggggtg gcgtgatcaa caccggtagc cgtctgatcc cgctcagtct cgcctggcgt
      361 tcgcttgccg atcatcaaag tgccgccgct aacgacattc gtcagatgat tcaggataac
      421 cgtctgcggc tgatgcaact ggcggggccc ggagcgcgct ttacctggtg gggtgaagat
      481 ggcaatggtg acgccttcct tacggcatgg gcatggtacg ccgactggca ggccagccag
      541 gcgctcggcg taacgcaaca accggaatac tggcagcata tgctcgacag ttatgccgag
      601 caggcggata acatgccgtt attgcatcgg gcgctggtgc tggcgtgggc acaggagatg
      661 aatctgccgt gcaaaacgtt gttgaaaggg ttggatgaag ctatcgcccg gcgcggaact
      721 aaagatgaag atttctctga ggaagacatc cgcgatatca atgacagcct gatcctcgat
      781 acaccggaat ctccactggc agatgcggtg gcaaacgtct taaccatgac gttgctgaaa
      841 aaagcgcagt tgaagtccac ggtgatgcca caggttcagc aatatgcgtg ggataaagcg
      901 gcaaacagca atcagccgct ggcgcacacg gttgtgctgc tcaatagcgg tggtgacgct
      961 acccagacgg ccgctatttt aagtggtttg accgctgagc aatccactat tgagcgcgcg
     1021 ctggccatga actggctggc gaaatatatg gcgacaatgc ctccagttgt tttgcctgcg
     1081 cctgcgggcg catgggctaa acataagtta actggagggg gcgaagactg gcgttgggtt
     1141 ggtcagggtg tgccggacat tctctctttt ggtgacgaat tatcgccgca aaatgtgcag
     1201 gtccgctggc gtgaaccggc aaaaacggct caacaaagta acattccggt gaccgttgaa
     1261 cgccagttgt atcggcttat ccccggtgaa gaagagatga gctttactct gcagccggtg
     1321 accagcaatg agattgacag cgatgcgctg tatctcgatg aaattacgct taccagcgag
     1381 caggatgcag ttctgcgcta tggtcaggtg gaagtaccgc tcccgccggg agccgacgtt
     1441 gagcgcacaa catggggcat ttcagtcaat aaacccaacg ccgcgaaaca gcaggggcaa
     1501 ttgctggaaa aagcgcgtaa tgaaatgggc gaactggcct atatggtgcc ggtgaaagaa
     1561 ctgacgggaa cggtcacttt ccgccatttg ctgcgcttct cgcaaaaagg gcaattcgtt
     1621 ctgcctcctg ctcgttatgt gcgttcctat gcacctgcgc agcaaagtgt tgcggcaggg
     1681 agtgaatgga ccgggatgca ggtgaaataa gtgaactggc gcagaatcgt ctggctattg
     1741 gcgttggtta ctctgccaac gcttgcggag gagacgccgt tacaactggt gctgcgcggt
     1801 gcgcagcacg atcaactgta tcagctgtcg tcatcaggcg tgaccaaagt ctcagcatta
     1861 cctgactcgc tcaccacgcc gttagggagt ttgtggaaac tgtatgttta cgcctggctg
     1921 gaggataccc atcaaccgga gcagccctac cagtgtcgcg gaaactcccc ggaagaggtt
     1981 tattgttgtc aggctgggga gagcataacg cgcgataccg cgctggtacg ttcctgtggg
     2041 ctctattttg cgccgcagcg gttgcatatt ggcgcagatg tgtgggggca atactggcaa
     2101 cagcgtcagg cacctgcgtg gttagcgtct cttacaacgc tgaagccaga aacctcggta
     2161 acggttaaat cgctgctcga ttcattagcg acgttacctg cgcaaaacaa agctcaggaa
     2221 gtcttactgg atgtggtgct ggacgaggcg aaaatcggtg tcgcatccat gctcggtagt
     2281 cgggtacggg tgaaaacctg gagctggttt gccgatgata aacaggaaat ccgccagggt
     2341 ggttttgctg gctggttaac cgatggtaca ccgctgtggg ttactggcag tggcaccagc
     2401 aaaacggtac taacccgtta tgcgacggtg cttaatcggg ttttgccagt gccgatgcag
     2461 gttgccagtg ggcaatgtgt agaggtggag ctgtttgccc gctatccgct gaagaaaatc
     2521 acagcggaaa agagtacgac ggcagtaaaa cccggcgtac ttaatgggcg ctatcgtgtg
     2581 acgtttacta atggtaacca cattacgttt gtcagccacg gtgagacgac cttactgagc
     2641 gaaaagggca aactgaaatt gcagtcgcat ctcgatcgtg aagagtacgt tgccagagtt
     2701 ctggatcgcg aggcgaaaag tacgccacct gaggcagcga aagccatgac cgtggcgata
     2761 cgtacttttt tacagcaaaa cgccaatcgg gaaggtgatt gcctgaccat ccccgacagc
     2821 agcgccacgc agcgtgtttc tgcttcgcca gcgaccacgg gggcgcgaac gatgaccgct
     2881 tggacgcagg atctcatcta tgcaggcgat ccggttcatt atcatggcag tcgcgctacc
     2941 gaagggacac tttcctggcg gcaagccacg gcccaggcgg ggcaggggga gcgttacgac
     3001 cagatactcg cgtttgctta tcccgacaac agccttagcc gctggggagc tccacgctca
     3061 acctgccagt tattacccaa agcgaaagcc tggctggcga aaaaaatgcc gcagtggcgg
     3121 cgtatattac aaggcgagac ggggtacaac gaaccagacg tgtttgcggt ctgtcgttta
     3181 gtctcaggtt tcccctatac cgatcggcag cagaaacggc tgtttattcg taacttcttt
     3241 acgcttcagg atcggctgga tttaacccat gagtatctgc acctggcctt tgatggttat
     3301 cccaccgggc ttgatgagaa ctatatcgaa acgctgaccc gacaattatt aatggactaa
     3361 tgctatgcga aagatttttc ttccgttgtt actggtggca ctttcgcctg tcgctcacag
     3421 tgaaggtgtg caggaagtcg agattgacgc accgctctct ggctggcatc cggcagaggg
     3481 cgaagatgcc agtttttcac aatcgatcaa ttacccggcg tcgtccgtca acatggcaga
     3541 tgatcaaaat atctcagcgc agatccgcgg caaaataaag aattatgctg cggcgggtaa
     3601 agttcagcag ggccggctgg tggtcaacgg tgccagtatg ccgcagagaa ttgaatccga
     3661 tggttcattt gcacgccctt atattttcac tgaaggcagc aacagcgtgc aggtcatcag
     3721 cccggatggg caaagccggc aaaaaatgca gttttactca acgtcgggta taggaacgat
     3781 tcgtgcacgt ttacggctgg ttctctcgtg ggatacggac aataccgatc tcgatctcca
     3841 tgttgtcacg cccgatggcg aacacgcctg gtacggtaac accgtgctga aaaacagtgg
     3901 tgcactggat atggatgtca cgacggggta cggacccgag attttcgcca tgccagcacc
     3961 agttcacggg cgctatcagg tgtatatcaa ctactatggt gggcgcagcg aaacggaatt
     4021 gacgaccgcc caactgacgc tgatcaccga tgaaggatcg gtcaatgaga aacaggaaac
     4081 atttattgta ccgatgcgta atgctggcga gctgacgctg gtgaaaagtt ttgactggtg
     4141 attaatgggg ttaccttcgc cgtcgatgtt caagaaaacg cccgataact ttcgctatcg
     4201 ggtgttttta ttgattaatt caaccgttca atcaccatcg caattccctg gccgccacca
     4261 atgcacagtg ttgccagccc cagcgtttta tcgcgtgcct gcatggcatg taatagtgtg
     4321 accagaatac gagcaccact ggcaccgata ggatgcccga gcgcgatggc cccgccgttg
     4381 acattcactt tctcagaatc aaagcccagg gttttcccaa cggcgaggaa ctgtgcagca
     4441 aatgcttcat tagcttcgat gagatcaatg tctgccagtt gcagccccgc cagttgtaac
     4501 gctttttgcg tggcaggtac tggccccata cccatcaatg cgggaggcac gccaccgctg
     4561 gcataacttt taatgcgagc caggggggta aggcctgctg ccagcgccgc agattcttcc
     4621 ataatcacca gagcggcagc accgtcgtta ataccagacg cgttcccagc ggtgactgtt
     4681 cctgctttat cgaaggccgg gcgcaatgca cctaacgctt cagccgttga attcgctttc
     4741 gggaattcgt cttgactgaa gacgaaggtt ttcttccgag tgacaacatt taccgggacg
     4801 atttcggctg taaaagcacc ggactcaatt gcggctgccg ctttacgctg tgaatgtagc
     4861 gccagttcat cctgcatttc acgggtaatt ccgtactctt tagccacgtt ttcggcggta
     4921 atccccatat gataaccatg ggtggcgcac atcaggccat cgcgcaggat tacgtcataa
     4981 acctgtccgt ctccaagacg ataaccagag cgtgcttttg catcgagtaa gtagggcgct
     5041 aaactcatat tttccatacc ccccgccaca atgctcttcg cctgacctgc ctgaattgcc
     5101 tgggcggcaa gcgccacact tttaagaccc gaaccacaca ctttattgac cgtgaatccg
     5161 cacaccgttt ctgccagccc gctttttaac agtgcctgac gtgccggatt ttgccccaac
     5221 ccggcttgca acacgttacc cataatcact tcatcaatgt gtagtgaatc gatttttgca
     5281 cgttcaatgg cggctttaat tactgtcgcc cccaggtcga tggcgctggt ggaagcgagt
     5341 gaaccgttaa aactaccgat agcagtacgt accgcactga cgatgacaca atttttcatt
     5401 ttatattcct tctgtctgta gggttgccgt cagaacagcg ttaaaccaat gacgaaaatg
     5461 acgccggaga agagcagggc agtgatgcaa tagcccatga tgtcgcggac accgagtccg
     5521 gcgattgcca gtgctggcag cgcccagaat ggttgttcca tgttcatcca ttgctcgccg
     5581 taggcgatcg ccattaccga tttaccgaga tcagcgccca gcgcctgggc tgcgggtatc
     5641 acgaaaggtc cctgaataac ccagtgaccg ccgccagacg gaacggcgaa gttaatcagt
     5701 gcagaactaa aaaaggtcat taccgggaag gtgtctttgt tcgcaacatt gatgaagaat
     5761 tcggtaatga gtccacccag accagaatgc tccatcatca gttggatccc agcgtagaag
     5821 gggaattgca ccagaatacc ggcagtactg cgtgctgccg cgctgatagc acgcatataa
     5881 gccattggcg ttttatgtag caacagaccc gcaatcataa acatcaggtt gacggtattg
     5941 atggtgatgt tgaagccatg ttcgctgaag tacatcgcca ggtaagcgat accgagtgcg
     6001 ccgatgatca acgtcagaat gcggctttct tccagtcgct cggatggtgg ggcatctttc
     6061 ggtagctgct tttgaaaatc agcctcttcc atgagtagtt ccggatcgat actcaccacg
     6121 tcagacggtt ttggcatcat catgcgggtg ataaatggca tcaccacaat caacgtcaca
     6181 gtgatgaaaa tgttaaaacc actgaacaga gtatcgccca ccgggatcag cccggcgata
     6241 tgctcaaccg ggttgcccgg tgttgcagcc aacagaggca ttgatccaga gaagccgcca
     6301 ccccaggtga gaaaaccaat gtaggcgcag gcaatgagca acggataatc agaaccgggg
     6361 actcgccggg cgacttcacg ggcaaacatt gcgccgacaa ccaaaccaaa tccccagttg
     6421 atgacacaag cgactgaacc gaagaaagtg accagcatga cgccctgtac gggcgtcttt
     6481 gcggcggagg cggcagtacg cagcaaactt ttcaccggag cagagctggc aagggcatga
     6541 ccggtaacga tgataagcgc catctgcata ccaaacgcca gcaagttcca gaaaccgtca
     6601 ccccacattt tcaccatgct gatcggcgtt tgtggtgtta accaaagcgc gatcacgaat
     6661 gttagcaatg tcagcaacat ggcaaagatc agtggatcgg gaagccatcg gctgacaaaa
     6721 cgcgtcataa aacgcgatat gcgaccaatc ataaatcacc ccgttgcgta ttcagatcgg
     6781 cggcgacttc aaaccgagct tctgttttgg cacgcacggt ggctaaatca cacccgtcgg
     6841 caatttcggt gagccacatt ttgccgtcaa taaaacgaaa gacagccagt tcagtaacca
     6901 gcatatgcac cgcatgttgc gcagtgagtg gcatggtgca gctgcgcaaa atttttgctg
     6961 aaccatcttt ggcgcaatgt tccatggcga tgatcacttt gcgcgacccg gtcaccagat
     7021 ccatcgcgcc acccataccg ggcaccattt tcccaggcac tacccagttc gcgaggtttg
     7081 cttcttcgtc tacttgcaaa ccgccgagca cgcaggcatc aatatgaccg ccacggatta
     7141 gcgcaaatga catggcgcta tcaaacatgg ctgcaccggg taaaacaccg cacggttgcc
     7201 cgccagcgtt caccagatct ggatgcgctg tcgtgaccgg gcctaaaccg aggaagccgt
     7261 tttccgattg cagagtgata tgaataccct ccggtaaata attggcgacc attgtgggta
     7321 aaccgatccc taagttaacg atgtcaccat cacgaagctc ttgcgccaca cggcgcgcaa
     7381 tacgttgttt cgcatccatt atttgctctc ctgtgaaacg atgatgtggt cgataacggc
     7441 accaggggtg acaatatggt caggttgcag ctcgccggtt tcgaccagtt catctggctc
     7501 taccagcgtg atatcagccg caagggctat cagggggtta aagttgcggg cgctaagttg
     7561 ataggtcagg ttgccaagtg tgtcgcaacg atgagcgcga attagcgcca ggtcggcgcg
     7621 cagtgggcgt tcgagcagcc aggttttacc gtcgagtgtc agtgtctgtt tgccttcctc
     7681 tacgacggtg ccgacacccg ttggggtgag aaaaccacca agtccagctc caccacagcg
     7741 aatttgctcg attagcgtac cttgcggcac cagaacgacg tccatctcac cagatatcat
     7801 gcgccgacct gtttccgggt tggtgccgat atgtgaagca atcactttgc ggactcgacc
     7861 attgacgatg agcggaccga tgccggtatc aacaaacgcg gtatcattgg ctatcaatgt
     7921 caggtcgcga acaccagatt ccagtaatgc ttcaaccagg cgggatggag tgccaatccc
     7981 cataaatccg cccaccatga tggtcatgcc gtcacgaaag aagccggtgg cgtcttgtaa
     8041 tgtcatcaat tttgttttca ttttttatcc ctcttgcata caccgttgtg ggtacagtca
     8101 ggcaatagca aggggagtgc cagaatgaga ttattggatt tagttattta actaattgaa
     8161 tatgaaggga taaattggat ggggatttat aaggagaatg agcggctatg cagaaaattg
     8221 cgcactgtgc aaatttctgc atagcaagtt ttggtgttat acatccgccg gatcgatacc
     8281 gtattcctgg agtttataca tcaatgcacg gcgactgatg cccagcatta aagcagtgcg
     8341 ggttcggttt ccttcttgtt gttccagcac ttccataatg atgcgttttt cgacgcgttt
     8401 aatttcctct tttaaattac gctcaccgac agaggctgtt tttacctcgc cagcattaca
     8461 gactggctga cgaatctgtg gcggaagatc ctcagaaaaa atgatcgggc ctgaattcat
     8521 cacgacggcg cgttcaataa cgttagaaag ctctcgaata tttcccggcc atgaccaggc
     8581 ggtaagcagt gacattgcca tcggatcgat gtcgataata tcgcgctgat tctcactact
     8641 gaatttttgc aaaaagtgat tagctaacag ggaaatatct tcccggcgat cgcgcagagg
     8701 cggcagtatt aaatgaataa cgttaaggcg ataaaagaga tcttcacgga aggtgccttc
     8761 ttttaccatt gcctgcaagt cacggttggt ggcagcaatg atgcggatat caacttttat
     8821 ggtctgatgg ccgccaatcc gttcaaattc ccgttcctgt agaatgcgta gtaatttggc
     8881 ttgtagtacc agcggcattt cgccaatttc gtcgaggagc agagtacctt cgttggctcg
     8941 ttcaaataat ccctgacgca aggtttgtgc accagtaaat gcaccttttt catgaccaaa
     9001 cagttcactt tcgagcaacg attccggcag cgccgcgcag ttgactttaa tgaacggccc
     9061 ctttgcccgc cgcgaattgt agtgaatcgc tctggcaatc aactctttcc cggtgccgct
     9121 ttcaccgcta atcaagacgc tggcctgaga aagggcaatt ttggcggtgt ctttgcagat
     9181 gtccatcatc gccgggctgt tggtgagaat gtgcccccat tgccagctgg cgctcagtgc
     9241 ctggtgcaga tgacggatct cttttttcat tgactggagt tgtaaagcgc gctgaacgat
     9301 taaattcaac tcatcgagat caaacggttt aataacatag tcgaaggctc cgcagcgtag
     9361 cgcttcgacg gcggtttcca cttccgcata ggccgtcatc agaataacgg gtgtccgggt
     9421 ctcatggctg cgcatctcct ttagtgcctt gatgccgtcc atctctggca tgcggatatc
     9481 catcaacacc acatcagggt gaatatcggc aaacaggtgt aatgctgtgc gtccgttgtt
     9541 cgcacaatgt gtttcgaatc cttgtagtgc aaaagcggtg ctcagcatac ggcgaacatt
     9601 atcttcatca tccacaataa ggatgcgatt aatagcagtc atacagtctg atttccctgc
     9661 gggttgatcg gtaa
//
LOCUS       DAESHI010000146         9645 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.148, whole genome shotgun sequence.
ACCESSION   DAESHI010000146 DAESHI010000000
VERSION     DAESHI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9645
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.148"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..667)
                     /locus_tag="KW011_003769"
     CDS             complement(<1..667)
                     /locus_tag="KW011_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBH7662004.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIQ
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDD"
     gene            complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="KW011_003770"
     CDS             complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="KW011_003770"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HBH7662005.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            5042..5647
                     /gene="azoR"
                     /locus_tag="KW011_003771"
     CDS             5042..5647
                     /gene="azoR"
                     /locus_tag="KW011_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBH7662006.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5698..7014)
                     /locus_tag="KW011_003772"
     CDS             complement(5698..7014)
                     /locus_tag="KW011_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HBH7662007.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(7004..8761)
                     /locus_tag="KW011_003773"
     CDS             complement(7004..8761)
                     /locus_tag="KW011_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HBH7662008.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKRRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHVKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8777..>9645)
                     /locus_tag="KW011_003774"
     CDS             complement(8777..>9645)
                     /locus_tag="KW011_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBH7662009.1"
                     /translation="ALLILATLINRFLLWRLPERKGGEVTLRIRTWWGIVICFSLVIS
                     GPRWMTLAFFALISFLALKEYCMLISVHFPRWLYWVIPLNYLLIGFNCFELFLLFIPL
                     AGFLILATWRVFVGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPSMNIQGGALLV
                     LFLLALTASNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMIASMIIGPLLT
                     PLNTLQAILAGLLIGISGFCGDVVMSAIKRDVGVKDSGKLLPGHGGILDRIDSLIFTA
                     PVFFYFIRYCCY"
BASE COUNT         2448 a         2628 c         2273 g         2296 t
ORIGIN      
        1 tatcatcgcg taaacgcggc agctcgccag tgagtagtga gagataacgc tcaaccggcc
       61 ataatccttg taactgatta ataaagatta cactgtctgc gttatttcct aactgaggaa
      121 cgaatccggg ataacttaac cagcgtggtg catcgggatt gtccccagtc atacggcgga
      181 acgtcgccat cgtgcgccgc tgcatggtgg ggtcctgaac aacgatagcc gtatgaactc
      241 gttctacggc ctgattcaat agcgcgatgc taaagcgtgc gttttcaccg cagtttgttg
      301 actggtcttc aatccagatt ttttcatgcg gaatgtgcca gaactgatga gcgatatccg
      361 cctggatggt cgcttctgct ctgccagtgg tgcggatagt gttgtagtgc ggatgctgtg
      421 cgatggcgct atacaaaaaa gttgtcgagt gaccgatacc accactaatc agtaaaggaa
      481 tttgctgatc gcgagcaatc ttacatgccg cgtcgatagt cggcataacc gcattgcctg
      541 caaggatcac gcaatcggcc tgatacggca cctcaccgga gaaatcatcc tgcgccagcc
      601 actgtccgat aacatttatg gcatctatag ttgccggcga aagcgtcgga aacggggtaa
      661 tgttcatggt tcctccttat cgttccctta agaatactgc gtgagataat agtgagaagc
      721 ggagcactct gaaaggagga agtcgtattt ccttaaccgc cactccgcaa gcctgacata
      781 tcttttaagg aaatgctgta gataaacatt attcatcttg cagcagagaa ttgtattttt
      841 ctgaattcct gaatttttac ccttaccgtt ttagaattaa taaaaaaccc ggcgctaaat
      901 gcttaccggg ttttttcttt atcaggcaaa tagcagggtt aaccgctaat ctgctccatc
      961 gcctgcaaaa tacgcttatc tgaaatcgga taaggcgtac caagttgttg agcgaagtaa
     1021 ctaacacgca actcttctat catccaacgg atctctttca cgtcttcatc ctcacgacgt
     1081 gcaggcggca gtttgttgat ccattgctgc cacgcctgct ggacgttttc gactttcagc
     1141 atctgagcac ggtcgcgatg tggatcaacc gccagttttt ccagccgttt ttcaatcgcc
     1201 tgcaaatatc gcagcgtgtc gcccagccgt ttgaagccgt taccagtgac aaaaccgcga
     1261 tataccagcc cgcccatctg cgctttaatg tcagaaagcc ccagcgccat cgtcatatcc
     1321 acccgcccct tcagacgttt gttgatattg aacactgccg taaggatttg ctcgacctgc
     1381 ttcgcaatat ccaccaccgt gtcgttcagt tcggcacgca ctttttcatg cagcgcagca
     1441 aagccttctt ccgtccagac cgggccacca ttggcgtcga tcaatttatc cacaccgcag
     1501 gagatacaat cgtcgatcag ctccagcact ttgccatacg ggttaaagta cagccccagc
     1561 ttggctttgt ttggtaactt ctcatgtaaa tacttgatcg gcgagggaat attcagcagc
     1621 agtagacggc gaagaccgtt ccacattacc tgtttttgct ccagcggatt atcaaacagt
     1681 ttgatcgcca cactgtcgcg ttcatccact agcgctggcc acgccttcac tttgtagttg
     1741 ccacgcttct gttcgtagct ttccggcagc tgaccaaaac tccagatatg cagtccgctc
     1801 tgctcgatac cgtcatccgc caccgcagac agcgtttcct gcactttgcc tttcagcgca
     1861 tctttcagat cttgtagcga gcgcccttct tttagcttct tgtttttgtc atccaccacg
     1921 cggaaggtga ttttcaggtg atcgggcacc tgatcccagt gccagtcttc gcggtcaacg
     1981 gtaacgccgg tcatccgccg taactcgcgc tcaaggctgt cgagcaacgg taactccagc
     2041 ggtgtgacgc ggcctaaaaa cgcttcggcg tagtttggtg caggcacaaa attacggcgt
     2101 accggtttcg gcaacgattt aatcagagca atcaccaatt cgcggcgcag accggggatc
     2161 tgccattcaa acccgctttc ctcaacctgg ttaagtaacg gcagcggaat atgtacggtc
     2221 acaccgtccg cgtccgcgcc tggctcaaac tgatagctca aacgcagctt gagattgccc
     2281 tgatgccaga agttcgggta atccagcttt ctgatttttt ccgccccttc tttgatcaac
     2341 atgctctttt caaagttgag caaatcaggc gtttcgcggc tgactttttt ccaccagcta
     2401 tcgaagtgac gagcggagat tacatcgtgg ctgatgcgct ggtcgtagaa ctcaaacagc
     2461 gtttcgtcat caaccagaat atcgcggcga cgtgatttgt gttccagctc ttccacttcg
     2521 gcccgcagtt tcaggttttc acggaagaat gcgtgacgcg tctgccagtc accttccacc
     2581 agagcgtggc gaataaagag ttcacgacat aacgccgaat cgatctggct gtagttgacc
     2641 ttgcgcgcag caacaatcgg caaaccataa acagtgactt tttccgttgc catcaccgcg
     2701 ccctgcgccc gttcccagtg cggttcgctg taggtgcgtt taatcaaatg ctgagcaacg
     2761 ggttctaccc attccgggtc gatacgcgca gcaatgcgcc cccacaggcg gctggtttct
     2821 accagttccg ccaccattac ccatttcggc ggctttttga ataaaccaga accggggaag
     2881 atggagaaac gcgcgttacg tgcaccggta tattcctgtt tatcggcatc tttcatgccg
     2941 atatgggaaa gcaaaccggt cagtaaggcg atgtgaattt cacgatactc cgccggttcg
     3001 ctgttgaccg gaatgccaag ttctttcacc acctggcgca actgggtgta gatatcctgc
     3061 cattcgcgca cgcgcagata gttgaggtaa tcggtacgac acaggcgacg gaaggcgttg
     3121 gaagaaagcg ccttttgctg ctcgccaaga taattccaca gatttacaaa cgcgaggaag
     3181 tcggattctt tgtcgtggaa gcgacgatgt ttttcgtccg atgcctgctg tttgtccatc
     3241 ggacgttcgc gcggatcctg aatggagagc gcggacgtga taatcatcgc ctcacgcacg
     3301 cagccatgtt tttgcgcttc cagcaccata cgcgccagac gtgggtcgac aggcaactgc
     3361 gagagctggc gaccgagcgg cgtcagttta taggcgctgg cctgttcatc cgtggtgatc
     3421 gcgcccagtt cttcgagcag acgcacgcca tcctggatat tgcgtttatc cggtgcttcg
     3481 acaaacggga acgcagcgat atcgcccagc cccagcgcgg tcatctgcaa aataaccgag
     3541 gccaggttgg tacgcagaat ctccggatcg gtaaactccg ggcgcgagag gaaatcgtct
     3601 tcggaataga gacgaataca gatcccttcg gacacacgac cacagcggcc tttacgctga
     3661 ttggcagacg cctgcgaaat gggttcaatc ggcaaacgct gcactttggt gcgatagctg
     3721 tagcggctga tacgcgctgt accggggtcg atgacgtatt taatccccgg tacggtcagc
     3781 gaggtttccg cgacgttggt cgccagcaca atgcgccgtc cgctgtgcga ctggaacacg
     3841 cggttctgtt cgctgttcga aagccgcgca taaagcggca agatttcggt atggcgtaag
     3901 ttcagcttgt tcagcgcatc ggcggtatcg cgaatttccc gctcaccact cataaagatc
     3961 agaatgtcgc cagggctttc ctgactcagt tcgtctacgg cgtcaaaaat cgcctgcaac
     4021 tgatcgcgct cggtgtcatc ggcttcttca acaatcggtc gatagcgcac ttccaccgga
     4081 taggtccgac cggagacttc gataatcggc gcattattaa agtggcgcga aaagcgttcc
     4141 gggtcgatag tcgcggaagt gataatgatt tttaggtcag gacgccgcgg cagcaactct
     4201 ttcaaatagc cgagcagaaa atcgatattc aggctgcgtt cgtgcgcttc gtcaataatg
     4261 atggtgtcgt actgcatcag caggcggtct tgctggatct ccgccagcag gataccgtcg
     4321 gtcatcagct tgaccatcgt gttatcactt acgtgatcgc tgaaacgcac tttgtaaccg
     4381 atgcaaccgc ccggctccgt tttcagctct tccgcaatac ggttcgccac cgttcttgcc
     4441 gccaggcgac gcggctgggt atggccgatc agccctttaa tcccgcgccc cagctccata
     4501 cagattttcg gtaactgagt cgttttacca gaacccgttt ccccggcgac gatcaccacc
     4561 tggtgatcac gaatcgcttc gagaatgtcc tgttttttct gactaaccgg taaattgtca
     4621 ggataagtaa tttccggtcg tgccgcttca cgcagcagga ctttccctgc cgcctggtca
     4681 atctctttcg ccatctcctg gaaaatggcc tgttgtgcat caggattttt aaccttcttc
     4741 acgccgtgca gacggcgaga aaaacgcagt ctgtcacgca gcatcagcga atccagccgc
     4801 tgctgcaagg ccgtaaaggt caatttttgt tgttctgtca taacgttagt gggcagtagt
     4861 ctgcctgatt aattttcttt gaaatgatag atatagagta ccacatcacg gctttctatg
     4921 cgttgttcaa taaattcgaa cataggcttc gatttattgc gctatctctg tggcaggatt
     4981 gtgaataaag tgtcaacaag caacggggca tcgcccattc aaacatctat aaggaaacac
     5041 catgagcaag gtattagttc ttaaatccag catcctggca gggtactctc agtctaatca
     5101 gttgtccgat tattttgttg aacaatggcg cgaaaagcac tccgctgatg aaatcaccgt
     5161 tcgcgacctg gctgcaaatc cgattccggt actggatggc gaactggttg gtgcactgcg
     5221 tccgagcgat gcgccgctga ctccgcgtca gcaggaagct ctggcgcttt ccgatgagct
     5281 gattgccgag ctgaaagccc acgacgttat cgttattgcg gcaccgatgt ataacttcaa
     5341 catctcaact cagttgaaaa actattttga cctggttgca cgcgcaggcg ttactttccg
     5401 ctataccgag aacggtccgg aaggtctggt aacgggtaaa aaagccatcg ttattaccag
     5461 ccgcggtggg atccacaaag atggaccaac ggacctggtg acgccgtatc tgtccacgtt
     5521 cctcggcttt atcggcatta ctgatgtgaa atttgtcttc gccgaaggga tcgcatacgg
     5581 tccggaaatg gcagcgaaag cgcagtctga cgcgaaagca gccatcgaca gcattgttgc
     5641 tgcataaccg gacacgatct tgatgtggac tgtagtccag tccacatccc aatccactta
     5701 cctgttttcc cataatttca gcatcgcttt gtgatcttcc ttaagcacaa tatgcgagcg
     5761 tctggctcga ataaagctaa tcgcttcatc aacggtttta cagtatccgt aacataacag
     5821 ccatgccgcc actaccagcg cactgcgcga taatcccagt gcgcaatgga ccagaacgct
     5881 gccttgctct tcgcgtaatg tttccagcat cgccacggcc tgtcggagct ccccctcttc
     5941 cggaaccacc agatctagca tcggtacaca gaaatagagt cgatcttttg tcgctcgtcc
     6001 acgagggaat tcaaaggtga cgtccagaac cgcattctgt gccggaatat gtcgtggaaa
     6061 cgcccctaaa taaacaccag cagtcatttt gctcaccggc tccaggcgac gacaaaacca
     6121 acgcatagat agccacatcc cgatgcgcca gggcaatgtc agccagtaaa cggcgggcgg
     6181 gagtttcccc tgactatctt tccctgttgt tatcgcgcca agcccaccgt acccacggcc
     6241 aataatgagt agcgataata ctggccaaca taaccagact gaccaccata actgaaccat
     6301 catcattagc tccatcaaca caatgcacgc gcacgcgcct acgacatatg gcagtgctat
     6361 tttgattcga cgttgatcag gtttctgata attccaccga cggtcgacgg gcaccatcca
     6421 gtcaatcaac atacctaccg ccagccctgt gatgacatca ataaaatgat gctgccatgt
     6481 cgtcagcgtc gaaatggcga tgagtaaaaa ccatccgccg cagactttac gccacctcac
     6541 agccagatgc tgacgaaagt gacgccaaag tagccagcag agaataatat gcagcgaagg
     6601 agactggtta taaggcagat caaacagttc aagttgcgaa aatagccatc ccgtcacccc
     6661 actcacttca ggacggataa aactaaactt cagcggatag agcaaaaaac cgcagcaggc
     6721 cattaccgtt gccagaataa gccggtggac aagtcggcgc tgttcgaatg tcgtgctaca
     6781 aacgaacagc gaaaatccat ataaaagatc cagactccag taaggaacaa tagtccaggg
     6841 aagaaaaggg atcgccgttt cccaaccgaa tacctgactg ggaatatcat ggctattact
     6901 gtcctgaacc gcggtgaact gattaagaga tccataggtg aagaagaaaa acggggccag
     6961 caacaataac cagccagctc cttgtagcaa tacgttccgg cgttcagtta tcacgacgca
     7021 ccgccatcga aaccgtaaaa atgccccact catcaatccg ttgtgtgcat ttatcaaatc
     7081 cagcatcatg cacgagcaaa tccatctccc cttgcgtacg cacgcgcatt acccacagtt
     7141 taccatcttt atgactggtt aacaccccgg caatcagctc cagttgtggg tgccacggct
     7201 gcccggtgta gatgagaatg ccacccggtt cgatggcatt ggcaagacct gcgagtgagt
     7261 tttttacctg ctcattttcg ggaaacagct catacagtcc agagacaatc gccagcgtag
     7321 gccgcggagt taacgcgctg agttcctccg ggttaaacgc atcgccctgt tcaaaacgca
     7381 cccgcccaga cattccccgt tgggcaatca tctcttgccc ctgtgcaaca tttaactcac
     7441 tgtaatcacg taataaaata tcgcttacgg caggctcgtt tgccagtgca tccagtacat
     7501 agcgcccatg ccctgcggca atgtcaacca cgcggacggc taaacctttg acgtggagat
     7561 cggcaacggc ttgtttaatc agtatttgta aatgagtttt gcgctggcga ataccgcgcc
     7621 agccaacact gttcaggtag attttgtcga ttaagcgccc gaatgcgtta ctgccttgcg
     7681 gttgattttg atagacataa tccagcgaac tgcctgaatc aaagccagtg ctcattccga
     7741 gatgcaggcc cgcagagtgc gtcccgaaca atttcatcgc tttacgcata aagcgatacg
     7801 ccaaatcaac cggcgataat ggcacgggtc cacctgaaag gaggcgccag cgatccgctg
     7861 atggtccagt gcggtcttca tgctgataat caaatttttg tgacttgtta gcatataaac
     7921 gacgaataaa gctttgcatt ttttcaaatg cctgcgccct gttctcttca cccaacgtgt
     7981 cgtgataaaa gcctggcagc agatgcagct ctttcagagg gctacgtaat ctctgataaa
     8041 aatcaatctg cggttggcga tgcaccacat agtcatcgcc tgatatcaga agttgcgtgg
     8101 ggagcgtaat cgccgccgca tctctaataa tacgttcaga cgttttatag agatcgagca
     8161 agatattgac ggcaatcgcc cgtgtgatca gcggatcatt attgaaactc gccacccgtt
     8221 gccgatcgtg ggtcaaatag cgtcttttca cataggaatt aataaaaaac agaccacgca
     8281 gacgatgcca tagcgtcagc gcaggacgtg ccagcggcac atacaattta accttaaagg
     8341 ccggagaagc cagcaccagc ccgcgaattg caggcgcata atcatgaacc catgtggcaa
     8401 ccagcactgc gccgacgctt tgcgcgatca caaccacctc ttccagtcca acctggctgt
     8461 cgctggcagc aaaacggaca aattcatcga catcctgcac tgaacgcgca agagatgggc
     8521 tataaccacg cggccccgaa gtttttccat gcccccgggc atcccatgca taaaaagcag
     8581 tatctggcat cgccagttca tcaacgatat gttgtagacg accagaatgt tcatggccac
     8641 gatgaaataa gacgatgacc tttttcgccc ctggctgtaa agcgggccag tgccgataaa
     8701 acaacgctgt attatcactg gtggtaaaaa aatgttcccc agagatgcgt gaattttcca
     8761 ttttctctat tttccttcag taacagcagt aacgaataaa ataaaaaaat accggagcgg
     8821 tgaaaattaa tgagtcaatc ctgtcaagga ttccgccatg tccaggtaat agtttcccgc
     8881 tatctttaac accaacatct cgtttgatgg ctgacatcac tacatcgccg caaaaaccac
     8941 taataccaat taacaaaccc gctaatattg cctgtaatgt atttagcggt gtcagtaacg
     9001 gtccaatgat catcgacgct atcatggtgg tgattacgcc gccgaccaga ccttccagcg
     9061 tttttcccgg gctgactttg gggaccactt ttcttctgcc gcaggatttc ccccataaat
     9121 actgtgcaat atcgtttgac gcggttaatg ccaaaagaaa taacaccagt aaggccccgc
     9181 cctggatatt catggatggc aacattaata accaggcggc atgactcaag gcgaaaacgg
     9241 tcattatcca gccccaaaaa atggcgctca cggtatgcag aaaaccggag gggtcaccca
     9301 caaaaactcg ccaggtggct aatatcagaa acccagccaa aggtataaat aacagaaaca
     9361 gctcaaagca attaaaaccg ataagcaaat agttaagagg aataacccaa tataaccacc
     9421 gcggaaaatg tacggatata agcatacaat attctttcaa tgccagaaaa cttatcagcg
     9481 caaaaaacgc caacgtcatc cagcgtggac ctgaaatcac cagtgaaaaa caaatgacga
     9541 tgccccacca ggtacgaata cgtaatgtaa cctcaccccc ttttctctcc ggtaaccgcc
     9601 atagcagaaa acggtttatc agcgttgcta aaattaacaa cgcga
//
LOCUS       DAESHI010000147         9621 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.149, whole genome shotgun sequence.
ACCESSION   DAESHI010000147 DAESHI010000000
VERSION     DAESHI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9621
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.149"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            223..2199
                     /locus_tag="KW011_003775"
                     /pseudo
     CDS             223..2199
                     /locus_tag="KW011_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311934.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2192..3328
                     /locus_tag="KW011_003776"
     CDS             2192..3328
                     /locus_tag="KW011_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBH7662010.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            3325..5325
                     /locus_tag="KW011_003777"
     CDS             3325..5325
                     /locus_tag="KW011_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HBH7662011.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEVARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5450..5911
                     /locus_tag="KW011_003778"
     CDS             5450..5911
                     /locus_tag="KW011_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HBH7662012.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5952..6422)
                     /locus_tag="KW011_003779"
     CDS             complement(5952..6422)
                     /locus_tag="KW011_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBH7662013.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(6469..7188)
                     /gene="yehT"
                     /locus_tag="KW011_003780"
     CDS             complement(6469..7188)
                     /gene="yehT"
                     /locus_tag="KW011_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HBH7662014.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(7185..8870)
                     /gene="btsS"
                     /locus_tag="KW011_003781"
     CDS             complement(7185..8870)
                     /gene="btsS"
                     /locus_tag="KW011_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HBH7662015.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            9092..9157
                     /locus_tag="KW011_003782"
                     /pseudo
     CDS             9092..9157
                     /locus_tag="KW011_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            9385..>9621
                     /locus_tag="KW011_003783"
     CDS             9385..>9621
                     /locus_tag="KW011_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBH7662016.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEW"
BASE COUNT         2353 a         2531 c         2469 g         2268 t
ORIGIN      
        1 cggccacgat acgtattgat tgaaggcccg gctgatttta atgaccgggt agacgaactg
       61 tttttagccc accagcttcc ggtagctatt tacagttatt gccagtatca ggacggtgca
      121 gcccccgggc gtggtgcctg gacgccattt gctgaatttt cgccggagtg gcaggcgcta
      181 caagccgcac gtcgtattca ggcacaaact tacttcatcg atttgccttg ctgggcgcag
      241 agtgaagaag tggacgattc gcctgatacg caagaggaaa gccaggcctt gctgctgcgt
      301 gccacccgca tggataacag cgacaccctg tgggatcact tgttcgaaga tgaaagccag
      361 caaactgcat taccctctgc actggcgcac tattttgccc aactgcgggg cgattccccc
      421 ggcgatgcac tcaatcgtca gcgcgaagcc tttatggccc gctggattgg atgggcgatg
      481 cagcaaaaca atggcgacgt gttagtcgtc tgcggtggct ggcacgctcc tgcactggca
      541 aaaatgtgac gcgaatgccc gcaggacatt aacacgccgg aattgccctc gctggcagat
      601 gccgttacag gttgttatct caccccctac agtgaaaagc gccttgatgt gctggcagga
      661 tacctttccg gtatgcctgc cccggtctgg caaagctggt actggcagtg gggcttacag
      721 caggcgggag aacaactgct aaaaacggtt cttacccgtt tgcgccagca caaattgcct
      781 gcttcgaccg cggatatggc tgccgctcat ctgcatgcga tggcgctggc acagttgcgc
      841 ggacataaac taccgttacg cactgactgg ctggatgcca tagcaggctc gctgattaaa
      901 gaagccctga acgcgccgtt gccgtggagc tatcgcggag ttattcatcc cgacaccgat
      961 ccgattctgc taacgttgat agacacatta gcgggtgacg gattcggtaa acttgcccct
     1021 tctacgccac aaccgcctct gccaaaagat gtcacctgcg aactggaacg taccgcaatc
     1081 tctcttccgg cggagcttac cttaaatcgc tttaacccca atgggctagc gcaaagtcag
     1141 gtgttacatc ggctggcaat actggagatc ccagggattg tacgccagca gggaagcaca
     1201 ctgacacttg caggcaacgg tgaagaacgc tggaaattaa cccgcccgct tagccagcat
     1261 gcggcattga ttgaggccgc ctgctttggt gccacactcc aggaagccgc acgccataaa
     1321 ttagaagccg atatgctgga cgcgggtgga atcggcagta tcaccacatg tcttagccag
     1381 gcggcgttag cgggtctggc gtccttcagt caacaattac tggagcaact cacattatta
     1441 atcgcccagg aaaatcaatt tgccgaaatg ggccaggcgc tggaagtgct atatgcctta
     1501 tggcggctgg atgaaattag cggtatgcaa ggcgcgcaga tattacaaac gacgttatgc
     1561 gcggctatcg atcgcacgct gtggctgtgt gaatctaacg gcagaccaga tgaaaaggag
     1621 tttcacgctc acctgcatag ctggcaggcg ctttgccata tcctgcgcga tctacatagc
     1681 ggcgttaatt tacccggcgt ttttctttct gcggcggtag ccttactgga gcgccgcagt
     1741 caggcgattc atgtcccggc gctggatcgc ggcgcggctc ttggcgcact gatgcgtctg
     1801 gaacatccca acgccagtgc cgaagcggcg ctgacgatgc tggcgcagtt atccccggca
     1861 caatccggcg aggcgctgca cggtttgctg gcgctcgccc gtcatcaact ggcctgccaa
     1921 ccagcattta tcgccggttt cagtagtcat ttaaatcaac tgagtgacga cgattttatt
     1981 aacgccctgc ccgatttgcg cgcggcgatg gcctggctac cgccacgaga acgcgggaca
     2041 ctggcgcatc aggtgcttga gcattaccag cttgcgcagc ttcctgtttc agcgctgcaa
     2101 atgccgttgc attgtcctcc gcaagccatt gcacatcatc aacaactcga acagcaggca
     2161 ctggcatcgc tgcaaaactg gggagttttc catgtctgaa ctgaacgatc ttctgaccac
     2221 ccgtgagcta caacgctggc gattaattct tggcgaagcg gcagaaacga cgctttgtgg
     2281 gctggatgac aacgcccggc agatagacca cgcgctggaa tggctgtatg ggcgcgatcc
     2341 tgaacggctc cagcgtggtg aacgctccgg tggattaggt ggctcaaatc tcaccactcc
     2401 tgagtggatc aacagtattc acacgctgtt tccgcaacag gtgattgagc ggctggaaag
     2461 cgatgccgta ctgcgctacg gcattgaaga tgtggtgacg aatctcgaca tgctggaacg
     2521 tatgcagcct tctgaaagcc tgctacgcgc cgttttgcac accaaacatc tgatgaatcc
     2581 cgaagtactg gctgccgccc gccggatagt gcgccaggtt gttgaagaaa ttatggctcg
     2641 actggcaaag gaagttcgtc aggctttttc tggtgtccgc gatcgccgtc gccgctcatt
     2701 tattccactg gcgcgaaact ttgatttcaa aagtactctg cgcgctaacc tgcaacactg
     2761 gcacccgcaa cacggcaagt tgtatatcga atccccccgc tttaacagcc gcattaaacg
     2821 ccaaagcgaa caatggcaac tggtcttact ggttgatcaa agcggatcga tggtggactc
     2881 ggtgatccac tctgcggtga tggcggcctg tttgtggcag ttacccggca ttcgtaccca
     2941 tctggtggcg tttgacacaa gcgtggttga tctcacggca gacgttgccg atccggtaga
     3001 gttattaatg aaagtacagt tgggcggcgg gaccaatatc gccagtgccg tggagtatgg
     3061 tcggcaactt attgaacaac cagcaaaaag cgtcattatc ctcgtgagcg atttttacga
     3121 agggggttca tcatcattgc tgacgcatca ggtgaaaaag tgtgtccaga gcggcatcaa
     3181 agtgctggga ctggctgcgc tcgatagcac cgcaacacct tgctatgacc gcgatacggc
     3241 ccaggcgctg gttaatgtcg gcgcacaaat agccgccatg acgccgggcg agctggcatc
     3301 atggcttgcg gagaatcttc agtcatgaat tcactacgtc cggaattatt agaactgaca
     3361 ccgcaggccc tgacggcgtt aagcaatgcc ggttttgtta agcgcagtct taaggaactg
     3421 gaaaatggca acgtcccgga gatcagccat gagaacgacg ctttaatcgc caccttcagt
     3481 gacggtgtcc gtacccagct ggcgaacggc caggcactga aagaggctca gtgcagttgc
     3541 ggggccaacg gtatgtgccg tcatcgcgtg atgctggtgt taagttatca acgactttgt
     3601 gccaccactc agtctacgga aaaagaagaa gagtgggatc cggcaatctg gctggaagaa
     3661 ctggctaccc ttcccgatgc tacccgcaaa cgcgcacagg cgctggtcgc taaaggcatc
     3721 accattgagt tgttctgtgc gccgggtgaa attccctctg cccgcttacc gatgagcgat
     3781 atgcgttttt attcccgcag cagtattcgt ttcgcccgtt gtgattgtat tgaaagcaca
     3841 ctttgcgaac atgtcgtact ggcagtacag gcctttgtcc aggcaaaagc gcagcaagcg
     3901 gaattgactc atttaatctg gcagatgcgc agcgaacacg tcacatcatc tgacgatccg
     3961 tttgccagcg aagaaggcaa aacgtgtcgt caatatgttc aacaactaag ccaggcatta
     4021 tggctgagcg gcatcagcca gccgcttatt cactacgagg ccgctttcag ccgcgcgcag
     4081 caagcagcgg aacgctgcag ctggcgatgg gtgagtgaat cgctacggca actgcgcgcc
     4141 agcgttgatg ccttccacgc ccgcgccagc cactatcatg ccggagaatg cttacgtcag
     4201 cttgcggcat taaacagtcg attaaattgc gcacaagagg tggcccggcg cgacagtatt
     4261 ggtgaagttc ctcctgtgcc gtggcgcacg gtcgttggct ctggcattgc cggagaagca
     4321 aagcttgatc atctgcggct ggtgtcttta ggtatgcgtt gctggcagga tattgagcat
     4381 tatggtttac gcatctggtt taccgatccc gacaccggca gtattttgca cctttcgcgc
     4441 agttggccgc gaagtgaaca ggaaaactca ccggcagcta cgcgtcggct gtttagtttt
     4501 caggctggcg cactagcggg cgggcaaatt gtttcacaag cagcaaaacg cagtgccgat
     4561 ggcgagctgc tgttagctac ccgcaaccgc ttaagcagcg ttgtgccgct gtcgcctgat
     4621 gcctggcaaa tgttgagcgc gccgttacgc cagccgggca ttgtggcttt gcgggaatat
     4681 ttacgccagc gtccccccgc ctgcatacgg cctcttaatc aggtcgataa cttatttatt
     4741 ctgccggtcg ctgagtgtat ttcgctcggt tgggatagca gccgccagac gctggatgcg
     4801 caggtaatca gcggcgaagg tgaagataat ctcctgacgt tatcccttcc ggcatctgcc
     4861 tgttctcctt ttgccgttga acgcatggcg gcgcttttgc aacaaacaga cgaccccgtg
     4921 agtctggttt ctggctttgt cagctttgta gaggggcaat tgacactgga accacgggtg
     4981 atgatgacaa aaacccgcgc ctgggcgctg gacgcagaaa ctgcgcctgt ggcaccgcta
     5041 ccttctgcca gcgttttgcc tgtgccgtct accgctcatc agttgctgat gcgctgccag
     5101 gcgttactta ttcaactgct ccataacggc tggcgctatc aggaacagag cgctattagt
     5161 caggcagagt tgctggcgaa tgacctctcc gcggtcggtt tttatcggct ggcacatgtg
     5221 ttgggacaat ttcgtaatac agaaagcgag gcacgggtag aagcaatgaa taacggtgtt
     5281 ttactttgcg aacaattatt ccccatgctt cagcaacaag gatgaaatag tgctttttac
     5341 taagagttct actccagttc cggactgctc acgccacggt attaggcata tcctatatag
     5401 cccctggtga gagtcaccag ttccttgatt aaataaaatg gagttttaca tgaaggcttt
     5461 caataagctg ttttccctcg ttgttgcatc tgttctggtt ttctctcttg ctggctgcgg
     5521 tgacaaagaa gaatcgaaga aattcagcgc caatctgaac ggcactgaaa ttgccattac
     5581 ctatgtctac aaaggtgaca aggtgcttaa gcaatcttct gaaaccaaaa ttcaatttgc
     5641 ctccattggt gcaaccacca aagaagatgc tgccaagaca cttgagccgt taagcgccaa
     5701 atacaaaaac atcgcgggtg ttgaagaaaa attaacctat accgatacct acgcgcagga
     5761 aaacgtgact atcgatatgg aaaaagtgga ttttaaagcc ctgcagggta tttcaggaat
     5821 caacgtttct gctgaagatg ccaaaaaagg tatcactatg gcgcaaatgg aactggtgat
     5881 gaaagccgct ggttttaaag aagtgaaata atctgtcggc ggccatgttt cgcatggccg
     5941 ccatacccgt cttagctttt cttcacatgc tggcgcgctg ccagtccacg cagaaaataa
     6001 cgtaaaaatt gatcgccgca ttcgcggaag tttttatgat ccggtgcgcg catcatcgct
     6061 gtaatttccg gcatcgaaac gcggaactgc tgttcggtga ggatcgccag aatgtcatcg
     6121 gttttcagcg aaaacgcgat gcgtaatttt ttcagcacga tgttgttatt aatgcgacgt
     6181 tccggctcca gtgccggagc agactcatcc ttgccgcgtt tttcataaat caggccattg
     6241 aggaatgacg acaaaacaat gtccggacaa cgctgaaaac cctcttcgtc ttctttacgt
     6301 agccagacgg cgatctgttc cgcggtggct tcgacattac ccagcgccag aatacgcacc
     6361 aggtcattat tattggcttt caaaatgtag cgcacgctgc gcagaatatc gttacttagc
     6421 atgaggcctt caggtgttga tgaggcaaaa agccatttta gcagtctttt acaggccaat
     6481 cgcctctttt aagcttttca gataacggcg gctgaccggc acggttaagc cattacgcaa
     6541 aatcaactcg gcctggccgt tatcttccag acgaatctcc tgtaaatgcg cgaggttaac
     6601 cagatactga cgatggcagc gcagtagtgg tgtacgactt tccagggtac gtaatgtcaa
     6661 ttcggtaaag ccctctttcc cttcgtggct ggtaacgtag acaccgctca tccgactgct
     6721 gacaaatgcc acatctttca tttgcagcaa ataaatccga ctatgccccg tacaagggat
     6781 aaatttcagc gcctgttgat tttccggtaa cagcgaaaca tcctgcttgc tgcgctcctg
     6841 acgcaatcgc gccagcgttt tctccagtcg cgcttcatca attggcttca gcagataatc
     6901 aaaggcatgt tcttcaaagg ctttaatagc gtattcgtca aacgcagtga gaaaaacaat
     6961 atacgggcga tgttccgggt caagcatccc caccatttcc agaccactga tgcgcggcat
     7021 ctggatatcg agaaacagca catccgggcg cagtttatgc accgcgccga tcccttccac
     7081 ggcgtttgaa cactctccaa cgatttcaat atcgctctgc tcctgcaaaa atacacgcag
     7141 gttctcccgt gctaacggtt catcatcgac aattaagact ttaatcatgc ctcgtccctc
     7201 catggtagtc gtaacgttat tcgggtgtaa ctatcaggct cacaggcgac gcttattcca
     7261 tagtcatcgc caaaccgttc acgtaaacgc ttatccacca gattcatccc cagcccactg
     7321 gcattggtta ccggttgata caaaccggca ttgtcttcga tctccagcat caaatgttgc
     7381 ccctcacgtc gggcgctgat tgccactcgc cctgtatcca gcagttgtga tgtcccatgt
     7441 ttaatggcgt tttccactat cggttgcagg gtaaacgcgg gcaattgctg ctgggataat
     7501 tcttgcggaa tagcaatgtt gacctgcaac cgcgactgga agcgcgcctt ttcaatttgc
     7561 agataagcgt tcacatgttc aatttcgtcg gcgagagtaa caaactccga aggccgcttt
     7621 aagtttttgc ggaaaaaagt ggaaagatac tgcaccagct ggctggcctg ttcgctgtcg
     7681 cggcggatca ccgctttaat ggtgttaagc gcattaaaca aaaaatgagg attcacctgg
     7741 gcgtgaagca gtttgatttc tgactgggtg agcatcgctt tttgccgctc atattgcccg
     7801 gcaagaatct gcgccgaaag cagttgcgca atcccctcgc ccagcgtgcg gtttattgaa
     7861 ctgaataaac ggtttttggc ttcatacaat ttgatggtgc ccatcacgcg ctgattttcg
     7921 ccacgcaacg gaattaccag cgtcgacccc agtttgcatt gcggatgcaa agagcaacga
     7981 taaggtactt cgttgccatc agcgtagacc acttcaccgg tttcaatcgc ttttaaggtg
     8041 taaggcgaag aaatcggttt gccaggtaaa tggtggtcgt caccaattcc ggtaaaggcc
     8101 agcaatttct ctcgatcggt aatcgcgact gcaccaatat ccagctcctg atacagcacc
     8161 tgagccactt tcatgctgtt cacttcgtta aacccctgtc gcaaaatgcc ttccgtcgag
     8221 gctgccactt tcagcgcagt ggcagaaaaa gccgaagtgt atttttcaaa catcgcgcgt
     8281 ttatcgagca atatacgcat aaacagcgcc gcgccgacgg tattggtgac catcattggc
     8341 gcagcaatat tactcaccag acgcaccgca tcttcataag gtcgggcgat cgccaggatg
     8401 atcagcattt gcaccatttc agcgacgaac gtgacggcac cggcggtaat ggggttaaag
     8461 actttatcag tgcgcccgcg acggatcagg atgctgtgta ccaggccgcc gagcaatcct
     8521 tcaacgatgg ttgagatcat gcaacttagc gcggtcatgc cccccatcga atatcgatgt
     8581 aagccgccgg tcagaccaac cagcccaccg acgaccggac cgccgagtaa gccgcccatt
     8641 accgcgccta tcgcacgggt attggcaata gaatcgtcaa tgtgcaaccc aaaccaggtg
     8701 cccatgatgc agaagatgga aaagacgatg tagcagagaa atttatgcgg cagacgaacc
     8761 gtgacctgca ttaacggtat gaataatggc gttttactca ttaaccacgc aatgactaaa
     8821 aaaacgcaca tctgctgaag cagcagcaac accagattaa aatcgtacat acccgcaaac
     8881 cacacttccc tttaaaacgc gtaacataca ttgcctgcgt ttaactttct ttgaactctt
     8941 gcagaaaaat gagaattcgt gagtacgatc actcaaaatc gcctggcaaa aataaaatca
     9001 ccctatagat gcacaaaaaa cgggcaaaac tacctggttc gcaaaactgc gtctaaagtt
     9061 aaaccgggac ctcgcgagca agggtgagac gatggcgctt tacacaattg gtgaagtggc
     9121 gttgctttgt gatattaacc ctgtcacgtt acgcgcgtga cgcaacattt ttaaaaatcg
     9181 acgaagatcg tccaggagcg ccgtttttca agggttggat agatgattcg tttaccatgt
     9241 cgacgaactt cgacgaattt cgccgataaa aagtgtccca tacatgcccc attgatgccc
     9301 cataaacaat atcaggaatt gacactggtt atccatacag ataaaaataa cacctgtaca
     9361 taaccacagt tacaggaggg tgttatgcgt gttgagatct gtatcgctaa agaaaaaatc
     9421 actaaaatgc caaacggtgc tgtggatgcg ttaaaggaag aattaacccg acgcatcagt
     9481 aaacgttatg acgatgtaga ggtgatcgta aaagccacca gcaacgatgg cctttctgtt
     9541 acacgcaccg cagataagga ttctgcaaaa acatttgttc aggagactct gaaagatacc
     9601 tgggagtctg ctgacgagtg g
//
LOCUS       DAESHI010000148         9501 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.150, whole genome shotgun sequence.
ACCESSION   DAESHI010000148 DAESHI010000000
VERSION     DAESHI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9501
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.150"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(149..1231)
                     /locus_tag="KW011_003784"
     CDS             complement(149..1231)
                     /locus_tag="KW011_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HBH7662017.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
     gene            complement(1228..3024)
                     /locus_tag="KW011_003785"
     CDS             complement(1228..3024)
                     /locus_tag="KW011_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBH7662018.1"
                     /translation="MNVQHFDFFTDNEFSNLERYQTCKAMAKGYAGNYKTGQSRLMHA
                     RSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAERTGELIAQFPAE
                     DAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDFSHASVIDPACGG
                     GAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLVLLESVLMPLCVK
                     VKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKYSRSLFGHANLYG
                     LFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYALDFVTDRDGVFD
                     DVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKGGATWLLPRVKDD
                     ACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPLVWAESITSAGFR
                     FSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAILPQGFIDETGGV
                     VVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVSAYELNSIPLPSL
                     EQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(3275..3631)
                     /locus_tag="KW011_003786"
                     /pseudo
     CDS             complement(3275..3631)
                     /locus_tag="KW011_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3689..4111)
                     /locus_tag="KW011_003787"
     CDS             complement(3689..4111)
                     /locus_tag="KW011_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBH7662019.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(4127..4852)
                     /locus_tag="KW011_003788"
     CDS             complement(4127..4852)
                     /locus_tag="KW011_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HBH7662020.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            complement(4874..5620)
                     /locus_tag="KW011_003789"
     CDS             complement(4874..5620)
                     /locus_tag="KW011_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH7662021.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(5627..6691)
                     /locus_tag="KW011_003790"
     CDS             complement(5627..6691)
                     /locus_tag="KW011_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HBH7662022.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            complement(6763..7188)
                     /locus_tag="KW011_003791"
     CDS             complement(6763..7188)
                     /locus_tag="KW011_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBH7662023.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(7185..7448)
                     /locus_tag="KW011_003792"
     CDS             complement(7185..7448)
                     /locus_tag="KW011_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH7662024.1"
                     /translation="MNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAKLTGEKIL
                     RWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
     gene            7560..8060
                     /locus_tag="KW011_003793"
     CDS             7560..8060
                     /locus_tag="KW011_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH7662025.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            8080..8274
                     /locus_tag="KW011_003794"
     CDS             8080..8274
                     /locus_tag="KW011_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBH7662026.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            8274..8564
                     /locus_tag="KW011_003795"
     CDS             8274..8564
                     /locus_tag="KW011_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBH7662027.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            8844..8996
                     /locus_tag="KW011_003796"
     CDS             8844..8996
                     /locus_tag="KW011_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HBH7662028.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            8996..9373
                     /locus_tag="KW011_003797"
     CDS             8996..9373
                     /locus_tag="KW011_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662029.1"
                     /translation="MRLTMEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARK
                     IKTAFVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRR
                     GLDGSYDHDVLQVLRTMVTSLGI"
BASE COUNT         2444 a         2491 c         2016 g         2550 t
ORIGIN      
        1 cacagaacaa ttattgcttt tgccgttgat gacaataaag tttttatcgc aggcgtatac
       61 cacggtgggc aaatctatga aaccgatttt ttatccagtg caccgtaaaa cccccatcct
      121 tcagggcgtg gcggatatca aactttggtc attttctctg gctgatcttt accggataac
      181 catcccgaag atcaattaag tgctccggtt cagaagcaaa ccatgcataa gatccccacg
      241 ccaattctgg tattgccttt ttaaaaggca gagcacttct gtccataaat gccgtaagga
      301 atgcaagatt tttctcgctg aacccagcgt caatagccaa atgagtgaga gtaacttttc
      361 gttccctgtt tataggccca tctgtcgcaa ctacttcagt gaaaactaca agcatgtccg
      421 aacccgttcg gtcctctccc aaatcaatca aaataatatc tggaagtgct tttgaggcat
      481 caatattcag ccccaatgct tttgccaata catcatcctg agcaacaact ttattcccgg
      541 attcagacaa ccagagcaca gttggattct taagaaaatt aggagcaaaa acttcgatga
      601 cggccttagc tattgtactt gacgggcccg gagctaaagt tctcttctct ccatttggga
      661 aagtgacgac tacggcatca gcagcaatca ctgcccctga tttcaataaa cgcaacctag
      721 caagagcggc tttgttcaaa tgattttctt gccaggaact tattgcactc agcagcgaat
      781 ctccttcaag cccggcatca aagagtgctg caaaagagcg attcaaactg taacgaggac
      841 gagaggatgt ggtgggtata cctttacgct caacaattgc attgcaaggt attaggccat
      901 tttttatagt ttcatcacgt attggctcac gcgtattttc cgcataccat gcatcagcag
      961 gccttatctt ttgtttagag aaagtcatct ttacccagct ttctctggac gcatcatcag
     1021 taagagcagc ctgcgaatct cccatactta caacctggct gggccttatc catcgcccca
     1081 gcccctcaac cgcaccagca taaaacatga caaaaattgt ttttgcagct atttcccgaa
     1141 ttaaatatcc acgattctca gtcccttcag ggaaaattaa ttgtaatctt tcccggataa
     1201 cattcaatgg gggaacaaca ggcagattca tgcaatcatc actccataga aaccagcgat
     1261 agttctctca ataatccgtc tgtggacgcc agcatcaatt aacgattgaa tctccatcac
     1321 ctgctccaga gaaggcaacg ggattgaatt caattcatag gccgaaacag caacacttcc
     1381 gcttatacac ctgaacgccc gatcgacaac atgcgaatta agtagcatat caataacatc
     1441 agggcgaacc gcagagaaca atccgtttga atatacaata ttaatatggt tttcaactac
     1501 tacgccacca gtttcatcta taaatccctg aggtaaaatt gccgctaaaa tccttctgtc
     1561 ctgttccttc gatgttgtcc gctgaacaag cacacattca gattttgtca caaggaaacc
     1621 ctgttgtggg gttatatcga tatagggaac atgatttttt ctgtctgcac taaaacgaaa
     1681 accagctgaa gttattgatt ccgcccagac taatgggtag ctattttttc ctttcgtcgt
     1741 acgcaactgc gacttaaatc gattccagac taactgacct gtagagactg agtatcccag
     1801 atcagctaaa cgcgtagaca tctgttttaa tacattaaga aaacaggcat catctttaac
     1861 gcgtggcaaa agccacgttg ctccaccttt ttctatatca acattaccta ttttctcaat
     1921 ttttgctttg ttcaacccct tagggacaag tgaggatacc tgggcccgaa ctttatgttc
     1981 tcccgccttg aatgctgtca acattgtttc ctggagaaca tcgtcaaaga ccccatccct
     2041 gtcagtgacg aaatcaaggg catacggagt agttttttcg gtcaaaaggg ttcgcagcgc
     2101 ggtgaaatac tgcccaccaa gaaaagaagt tggcgtaaga aatgcaataa ctcctgtttt
     2161 ctctttaacc atgcgaacag ctaagtcggt gaacaatcca tatagatttg catgaccaaa
     2221 taacgaacga gaatattttt ctctcgtttt aatatcgaga gtgacacgtc cgtaaggcgg
     2281 attccctacc acaagatcat aaccagaaaa tttatcttgc tgaagcgcat cagcaacaat
     2341 aattgtatct tcaggcaatc tacgttttac ctttacacat agtggcatta gtacagattc
     2401 gagcaatacc aaactcatcc aagccgcaaa tggatcgatt tctatcccct ttaaacgacg
     2461 actgattctc ctcaacatcc attcagggga agaccccttg tcttttttta acattcgaat
     2521 ggctactggt gccaaaaatg caccgccacc acacgcagga tcaataaccg atgcatgaga
     2581 aaaatcaaca ccagactttt cagccaggtc caagagacgc gctaccaaag gtgggggggt
     2641 atagtaagcc cccaattccg aacggtaagc ggatggcagc attaccgtat aaattgaccc
     2701 aatcagatat ccagcgtcct ccgccggaaa ttgagcaata agctcacctg ttcgttcagc
     2761 gagctcgcgt gcatcacatg gaatgtcttc tatgtaaaca gtgtgcggta ctgccctcat
     2821 tttaatgctg cttttttgct tttttgcaag ggtatcccaa tatgcagcta cgatgctgaa
     2881 gcagtatgag cgggcatgca tcaaccttga ctgcccggtt ttgtaattgc ccgcataacc
     2941 tttggccatg gctttacaag tttgatatct ttccagatta gaaaactcat tatctgtaaa
     3001 aaaatcaaaa tgttgcacat tcataatgta aaatcctaat gtatcaaaca tgcgggccat
     3061 aacatctcat ggtggcgcat agcaaactgg cccgaagaat atcacgcacc aagtcgatct
     3121 cataacccgt tatgacaata aatctttgta gctgaactca cttggtgatt tgtcaatcat
     3181 tcctggcctc cagttcgtcc tgaatctctt tatcgagttc accgtatttg ttagtcctca
     3241 tcactcacga cttgacttcc agaaataacc ttgattattc ggggctatat tttgtcaccc
     3301 tgcgtatccg cgctttcgca ttacgctcaa tctggattag cttttctata ttttttcgcc
     3361 tttcccgctc ctcctagcgc aagagcctta catcatctgc cagtctggtt tctcttttcg
     3421 ccacagagag catccagtca aatggctcca caactgcacc gcaaatttta cagcggacct
     3481 gacgctcttt ttcgtcaacc cggacagaag cgtgatggca atatggtctt tccgatggct
     3541 cataaagaaa attaacctga ttacgagggt tatcctcttt taccggaaat aaaacaatat
     3601 tgcttaactc atcttctggt tttatttcca tgctcctctc ctttgatgcg aatgccagcg
     3661 gtaattgaag cctgatagct aatttcactc acagtaccgc ctcctgaaaa tcaccctgat
     3721 agaaagccgg tacacgctgc ataacttcac tcttccggca ctcgcgacag attatgttct
     3781 gatgtctgtc gtagcggcgt atttctccgt ctggtaatga ccagataagg tccggatcaa
     3841 ccacagatgg tttcttcagc tttgccctcg agagtttttt gcgggcgttt tgccagtcct
     3901 tacgagcctg ttcagacggg aataacccgt agccagagtt gtatacatcc ccactggcaa
     3961 ccagctctct ggcgagaacg cttattaaat atcttgtcgc cccggtttta gcttccagtt
     4021 gtcgtaacgt ctcgcgccca ctctggcgta cgagttcaac aacctgcccc ttaatttttt
     4081 cccgctcttc ctgtgtaaat acttttgcca taagcgcccc cggcaatcac ttttccgaca
     4141 caatacgacc ggaggaatcg acaatctgtc gaacaatatc ccggtgcttg ttcagctccc
     4201 gcaacgcggc gcagacacgc tcccacttct ggacatgact tttcgcccga cgcagttcgc
     4261 ggtttgccat atgcagcgat ggtaaaacca ggtcatccgc ttgcgtttcg gtgaacgatg
     4321 gcaacgactg cacaatgtcc gccacagttt ctgttttaat ttcttcctgt gttgcagctt
     4381 cctgtaccgg taacgcaaca cctgctggct gaggaaagac tttaccatcc gtttccgcta
     4441 tggatgcggc tttcggctct gccggtaaat cagcgcccgg tatgcagtac cgaaatttac
     4501 cgccctgatt cacgcgaatc agacgccctt tgctgattgc catggccagc gatgaattcg
     4561 cccggcggga ggtaatcccg aacatcagtg ccagctcatc cgccgtttgt gggccatgtt
     4621 gttcaatcgc ctcagtcagc atttgcgctg tcactttcgg taccggtgac actggttcac
     4681 tttcaccagc ctgaatcagc caccacatcg aacccttgtt atccgcttca ccacggcgct
     4741 tcagtttcca cagttcgttg accgcatctt cacggctgat tccaaggcgg gacgccacca
     4801 catgtgaaga ggctcttttc agtgctttca gtgcgtcaaa tacagtttcc attaaaacat
     4861 cctccaacaa aaattacttc acaactcccg gaaagctgac atttgaacgc cagctatccc
     4921 agttaaacgt cacccaccga caaccgttca tggtcatgcg gtccataatc ctctcaccaa
     4981 gaagcgtgct cattgcggca tgattcaggt ttgttaacat cccgacactg cacagtgatg
     5041 ctgtccggcg atcaattatc tggtgcaata ccacctgctc gtttttcgtc tcccgctgaa
     5101 tgcctatttc atccaggacc agcaaatcaa ccccgcaaag ctcctgtaaa aatttttcac
     5161 cggatttgcc gttgtcgtag ctgtcatgca acacgctcat gacatcagac tcggtgacga
     5221 taatcacgct gcgccccttc gccatcagcc ggttacccat cgccgctgca aggtgatttt
     5281 tcccggtgcc ggttttaccg ctgaacacaa aattcgtgca cccggtcatc agttcgtcag
     5341 ctatggattt ggcctggctc agcgcgtatt tttgcccgtc gttctgcacc tgataattcg
     5401 caaacgagca tttgctgtgc agaggctgga tgcccgaacg attcaggatt ttttccaccc
     5461 gcaactggcg attctggcgg ttaatctcct cgctgcgttt tcgtccttca gcaagttgcc
     5521 attcccgcca ctcctccacc gtccggtacg gtggaaccgc cccctgtggt gcaagtctgt
     5581 gaatacgttc aagaaccccg gctgccgcaa tgtttttcat gccacatcac cccctgaatc
     5641 ccggcggaat ttcggtatcc ggttcagaaa tatgattcac acaacgctgg ttgttcgtgc
     5701 cgcttaccgg gagcaaccag gggttttcaa aattccggtc cggcccaaaa aacgtcgtcg
     5761 ctcgctgaac aaattccgtt cccgctttcc cggtcgccgc caggtatctc gcgtaacgcc
     5821 tcacaccatc cagcatggtc tctggtggca ccccctcgcg caatctggcc ttccaggcac
     5881 tgaatgcgga tttcttcggg tttgccccgg cacgcaacgg gtattcccgc cagacctgtt
     5941 cgaacacatc cggataatcc actcgtccca caggctgccc ggtgttttcc ggggctaccc
     6001 gatcggcttc ccgctgaatg gcggaatcgg cttcaggctg ctgcagttgg agtgattgct
     6061 ccggccttgc ggtcatcacc tgctgcacag cgcccgaatc ggctttcagc gcatacgctg
     6121 aatcggcttc cggtgtcgag cctgctggct gaccaggatt gacggtctga acatcccctg
     6181 cctggttcgt ggcgtttttt acgccatgga ccatagtgtt ttgatcttct ttatctgtat
     6241 ctttatctgt atctgtcgtg actcgtcgtg acatgtgcgt gacatttcgt gacgcgccgt
     6301 gacaatcgcc attttgttcc cgctttcttt ccctctctcg ctgcgccctc ttgcgctctg
     6361 caggagattt tgcggtttgc gaaatattgc cgttgtcctc tttaagcacc tggcgttttt
     6421 cccatccagt gattaaatca ccatcaagta cccgcccctg catcgtctgc aaaattgaat
     6481 caattacctc ttctgtcacg tcgagcgcac ttgccaaatc ttctgtcgtg acatcaatgt
     6541 gacctcgcgt gacatttcgt gacgcgctca ccaggaggtg gatatacact gccatcactg
     6601 ttgcaattgg ctgccctgac accctggcaa ttgttcgcca cttagggtca tttggcatgt
     6661 catgccataa tctgagccag gcgttagcca tactcacctc ttctgatacc gaatcttttt
     6721 actcacgagt tgccgggagc gattcgatat ggctattgtc agtcaatgta ctgccacagc
     6781 atttcctgcc gggccaccac ggttcatctg attgaaaccg gcgattgcca ctgcgacaaa
     6841 atcatcagcg tctctcacca gtcgttcatg cgtctccacc agttcccgaa aataagctga
     6901 actgtggctg cgcattcgag ccaccagcgg aggtggcatt gccctttcga tcgcaggtaa
     6961 caacgcctga attttttcaa cagcgtcagg ggtgtcttta tccagccaac ggaaaatctt
     7021 ttgggtgttg agataaagcg tgttgtgctt gctgtcgtta tgcagaaccg gaaacgtcat
     7081 tcccagttcg aaataaaccc tggttatttc agctgccgga actttttcac cgtccggatg
     7141 cgcccaggct ttcatcgcca tgcggatgtg ttcatgcttg attttcatga atcaaccccc
     7201 gccgatggtt gtgtgttagc ctgaaactca gcaggcaagc cgtcagttgg gttggggtag
     7261 gttttactgt caatctcatg tggggtcacc acccatcccg ttgcttcaca ccaacgtaat
     7321 attttttctc ctgtaagttt tgcacgcccc gttataacgt ggctgaccat cccttgggat
     7381 gcgccaacaa tctcagcaaa tgaactctgg gttatatgaa aatgatgcaa atattctcca
     7441 agattcataa tccgccctat gtgatgtgtt accaatgcat aattaatagc actgttattt
     7501 ttaaaagtaa atagtgatgc tatttcaaag tgattagcaa tcttattaga attggatgta
     7561 tgaaaagaaa atcattgtca gacaccgacc tcaacgccgc cagaagactg aaagaaatct
     7621 ggatggcaaa gaaatctcag ttgggattga ctcaggaacg tgcggcggaa attatggggt
     7681 tttcaacaca aggggcggta agtcactacc taaatggtca aaccccacta aaccttgagg
     7741 ctgttctaaa gtttgctggc ttactgaaag ttccccctga gtcaatcagg ccagacatgg
     7801 tagatttgct gcaaattgca aggatgtatt gtcgagaacc acagcaagat aacatagtcg
     7861 cattaccagc agacacagaa caaacagagg atgaactacc gtttgccgta gagccgatgg
     7921 aacgagattt aatccatacg ttcagagctt ttccgaaaga agatcaagaa cacatgcttc
     7981 aggagatgaa agataaaaaa gaatcaatgg atcgtacggt tgcgcggtgg ttagctgcac
     8041 aaaaaggccg tcgcgcctaa atcagaggag atatgtaaaa tgagtacagt cctttcccca
     8101 atggtttcag aatttgagac agttgagcaa gaaaatagtt acaacgaatg gctgcgggcc
     8161 aaagtagcag caagcctcgc ggatccccgc ccggcgattc cgcatgatga ggtaatggcg
     8221 gaaatggaac aaattattgc ccaaattgct gctgcaactc acagaggtga ataatgttac
     8281 ccgttttatg gctaccatct gctcgtgatg atttgcgcca gatcgtagcc tacatagcta
     8341 aagaaaatcc cgcggcagca cgcagaatga aaattcgaat tgaaacatcc gttttgccgc
     8401 tcacagagca tccttacctg tatcctccaa gcgagcgagt tcttggtctt cgtgaaattg
     8461 taactcatcc taactacatc atcctttacc gagtaacagc atcaagcata gaagtagtaa
     8521 atattgtgca ttctcgaaga cagtatccag gcaaaaacag ttaattattc ccgccgacag
     8581 aaccacctta gggtggtttt ttcttgcccc aacaaaaaac aatagcgatg ctatttacat
     8641 caaaaaaaat aatgatgcta ttgtttcagg cgtcaaccca ccccgcccca cagaatgcag
     8701 ggcaatactt cgagttacca gacagtggtc aggggttaag tagccagccc gaggcgtaag
     8761 aacatgacgg cagggttcaa tctttaacta tgcagcaggt tttttgttcc gccaccccgg
     8821 cgttaagggg aaatgaggtc agcatggata ctatcgatct tggcaacaac gaatctctgg
     8881 tgtgcggcgt gttccctaac caggatggaa cgtttaccgc catgacgtat accaaaagca
     8941 aaacgtttaa aaccgaatct ggcgcgcgtt gctggttagc cagaaacact gactgatgag
     9001 gctgacgatg gaatttaaag atttaccgtc tgatgtacag aaaacagcag ctcatacatt
     9061 gcattctgtg ctgcgagaaa tcgggaaaga tattgcaagc gagccagcaa aagatctggc
     9121 ccggaaaatc aagaccgctt tcgttgagct ttataatgtt ggcactgact ctgaaactgt
     9181 cgagaccaag accgtaagtt caccaatatt ctcacttgag ccaagcgtat tatcaggtga
     9241 aatatgcacc gagatttcca gcgaactgct tccggtaata cgagaagcaa ttcgccgtcg
     9301 cggtttggat ggaagttatg atcatgacgt tctgcaagtt ctcaggacaa tggtgacttc
     9361 actggggatt tgatccctgc gtcctctaag cttttgatat agcggtcctc caagaagcgg
     9421 taaattttgt cgaaatcatc ttttccctct ggtttattca gaggattaca ggacccgtaa
     9481 gtagcggcat acatttcaac c
//
LOCUS       DAESHI010000149         9496 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.151, whole genome shotgun sequence.
ACCESSION   DAESHI010000149 DAESHI010000000
VERSION     DAESHI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9496
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.151"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            122..952
                     /locus_tag="KW011_003798"
     CDS             122..952
                     /locus_tag="KW011_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HBH7662030.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIY
                     LPPLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="KW011_003799"
     CDS             complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="KW011_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HBH7662031.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2081..3541)
                     /locus_tag="KW011_003800"
     CDS             complement(2081..3541)
                     /locus_tag="KW011_003800"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HBH7662032.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="KW011_003801"
     CDS             complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="KW011_003801"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBH7662033.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5146..6489
                     /gene="gntP"
                     /locus_tag="KW011_003802"
     CDS             5146..6489
                     /gene="gntP"
                     /locus_tag="KW011_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HBH7662034.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDTATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGQLELVNSVVGLIIVLIISMVA"
     gene            complement(6653..7555)
                     /gene="fimH"
                     /locus_tag="KW011_003803"
     CDS             complement(6653..7555)
                     /gene="fimH"
                     /locus_tag="KW011_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HBH7662035.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7575..8078)
                     /gene="fimG"
                     /locus_tag="KW011_003804"
     CDS             complement(7575..8078)
                     /gene="fimG"
                     /locus_tag="KW011_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HBH7662036.1"
                     /translation="MKWRKRGYLLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNSGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8091..8621)
                     /gene="fimF"
                     /locus_tag="KW011_003805"
     CDS             complement(8091..8621)
                     /gene="fimF"
                     /locus_tag="KW011_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HBH7662037.1"
                     /translation="MRNKPFYLLCAFLWLAVSRALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8631..>9496)
                     /gene="fimD"
                     /locus_tag="KW011_003806"
     CDS             complement(8631..>9496)
                     /gene="fimD"
                     /locus_tag="KW011_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HBH7662038.1"
                     /translation="KSYCLNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLA
                     GVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQL
                     YYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYAT
                     EYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPL
                     PFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQ
                     QLLTQLSAECR"
BASE COUNT         2259 a         2484 c         2376 g         2377 t
ORIGIN      
        1 gtgtagagtt gatatcacta caacagaaac atgcatgtca tttgtttact tctcttttta
       61 ctacagaaaa atttatctat ttttctaaac atcagtgaaa tactcataaa aggtaactat
      121 tatgtctatg ccattaagca atgcccttca gagtcaaatt attacagata atcacttcct
      181 tcatcaccca atagtcgaca gtgagtttac acataaatta aaatatgcac gcatggattt
      241 ggaaaacatc tatcttccgc ctcttactcg tggcaataac cataattacg atggaaaaag
      301 cgctgtcgaa attagaaaac ttgatattag taaagagtcc tggccattta attatgtgac
      361 aggagcatgc agagaatccg atggtattac aacaacagga cgtatgctct atcggaactt
      421 gaaaatcacc tcagctttag acgaaatata tggtggcata tgtaaaaagg cgcatgctgc
      481 cacagagtta gcagaaggtt tacggctaaa tttatttatg aagagccctt tcgatcctgt
      541 tgaggactat acagttcatg aaataacatt aggccctggt tgcaatgttc ctggttatgc
      601 cggaaccacg ataggatata tttcaaccct tccagcttct caggcaaaaa gatggaccaa
      661 tgagcagcca aggattgata tctatattga tcagataatg acggtaacag gggtagcaaa
      721 ttcttccgga ttcgcccttg ccgcattact taatgctaat attgagttgg gtaatgaccc
      781 aataattggt atagaagcct atcccggtac cgcggagatc catgcaaaaa tgggatataa
      841 agttatcccg ggtgatgaaa atgcaccact caaacgtatg actctgcaac cgtcatctct
      901 tccagaactg tttgaattaa aaaatggtga gtggaattat attggtaaat aactaccagg
      961 aagaatgagt actaacattc aggttataca gatagctaaa caataccctg caatttacaa
     1021 agtaatactc attgcgactt cttaatttaa gaaagcgcaa ggaacattta cccttgcgct
     1081 tattataata agtcaggcgt caactttatc cagcggccat gaatcaaaca gatagccatc
     1141 aaaataaata tcgtcaacgt tcgagaattc cagcagacgt tgcttaacgt tttccagatg
     1201 ccgccacatt gccagcttcg ccgctcgggc atcttttttg attaacgcgg cgaggatctg
     1261 tttgtgatcg cccaaccact ctttgcgata caggctgtca tccagatggc tgtgcaactg
     1321 gatccacatt ggattgtttt cccgccactg ccaggactga cggaacagct ccaccagcat
     1381 gctgttatgc gttgcttcgg caatggcgag atggaactgc atatcaccac tttcgctgct
     1441 gcccggcgcg ctggaagcca gctcacgctc ttccagttgc aatgcctgac gcattttgac
     1501 gatatcttcg cgggtagcct gcaaagcggc aaattccgcg atgttacttt ccagtaactg
     1561 acgcgcctgt aacagctcaa aaggaccggc atcattgcag acgttagcat ccggactgtc
     1621 tgtgttgtgg ctgcctgagc tatcaagaac atagataccg gcaccccggc gcacttccac
     1681 cagccctttg atctccagca tgatcagcgc ttcacgtacc accgtcctcg tgacatcaag
     1741 catttctgca atttcacgct ccggcggcag ccgttcgcca ggattgtatg gcgtctttat
     1801 gatcagatcg cggatcatcg ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga
     1861 tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt tttgccggat gcggcgtgaa
     1921 cgccttatcc ggcctacaac gggcaatttt acggcacccg ttaatctcaa actataccat
     1981 tagcactgga agttggcacg tacaggtggg caaaaaaacg aaaactggtc aaccaaatat
     2041 cagtttgatt gaccagtaag tctgtagggt aatcagttag ttacagcgca gccacacact
     2101 cgcgcgcacc gcgttcgcac agctgctgat atgccgccgt caccgcgcca acaaagtcgg
     2161 cattctgcgg cagatcatcg gcaaaaatgc cgctcaggcc cagcagcgct ttcacgcggt
     2221 ctgcgccctg atactgcgcg ttaatcttct ggaactcggc cagcatcggg tcaaccacgt
     2281 caatggcatt accctgttca tccacgccct gggtgtagcg catccagcca gccacgccca
     2341 gtgccaggtg acgccagctg ccgccgtttt gcaggtgcag acgcaccgga tccagaagac
     2401 gctgcggtaa cttctggctg ccgtccattg caatctgcca ggtacggtga cgcagagacg
     2461 ggttgctgaa acgctcgatc agcagcgtcg cataggcgtt caggtctgta ccttccggca
     2521 tcgacagcgt tggcgcttgt tcctgcatca tcagggcaaa ggccgctttg cgataatccg
     2581 gattagtcat ggtgtcggca atggtttcat agccgccgag gtaaccgagg tacgccagga
     2641 aagagtggct gccgttcagc atacgcagtt tcatcatttc gaacggcaca acgtctgcaa
     2701 cgaactgtgc gcccacttta tcccagtccg gacgaccatt aacgaagtta tcttcaatca
     2761 cccactgacg gaacggttcg caggcaatgg cgcacgggtc gtaaacaccc agctggtcag
     2821 caatttcctg taaggtttct ggcgtcgccg ccggaacgat gcggtcaacc atggtgcacg
     2881 ggaaggtgac gttctcttca atccatgccg ccagctgcgg atcacgtgcc tgagccagcc
     2941 ccagaaccgc gaccttcgcc acatgaccgt tttcacgcac gttgtcgcag gacatcaccg
     3001 taaacgcttt cagccctttt tcacgacgca gacgcaaggc ttcgacgatg taaccaatcg
     3061 cggacttcgg cgcagtcggg ttttccagat cgtgcttgat cagcgggtta ttgagatcca
     3121 gctgaccgct tgccgcatcg gcgcaataac ctttctctgt gaccgtcagg gagacaatcg
     3181 ccgtctgcgg acgcgccatc gcgttgagaa taccttcaca accgtcgatt tccggatgca
     3241 gggcttcttt catcgaaccg ataattttca gctcggtgct ttctgcgcct ttttccgcca
     3301 cggtgtacag cagttgctgt tttttcaggt tttcaatcag cacacggtcg ttgcctggca
     3361 tcaggttaac ttcgcagata ccccagtcgc tgtcggtact ttccagcaga tgatgggtgt
     3421 acagcgcctg gtgcgcgcgg tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca
     3481 gacgagaatg atcccatgac gggcgggcaa ccggcagatt gctgtcaaca atagtagtca
     3541 tcgtaaactc cagaatccgt cttcagagag tgacggcaag gaagaaggaa ccggcacggc
     3601 gcgcagccat gccggtgggt atcaacggct aaagaaagcg cgctggatcg ccagttcgac
     3661 accgcgaact tcagccaggc ctttcagacg accaattgcg gagtaacctg ggttggtttt
     3721 cttcttcagg tcgtccagca tctgatgacc gtggtccgga cgcatcggga tcaggtcttc
     3781 tttgccttcc gctttacgac ggtgttcttc ttcaacaatc gctttcacca cttcgtacat
     3841 atcaacgtca ccgttcaggt gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat
     3901 ggtggagcgc agatgggtga agtaaatacg cggaccgaac tgcttgatca tatcaaccag
     3961 atcgttgtca gcacgcacgc cgtaggaacc tgtgcacatg gtgaaaccgt tcgccatgct
     4021 gtttacggta tcaaccatcc actgcatatc ttcaatggtg gaaacaatgc gcggcaggcc
     4081 gaggatcggg cgcggcggat cgtccgggtg aacagccata cggacgccaa cttcttcggc
     4141 aaccggaata atcgctttca ggaagacggc aaagttttcg cgcagtttgg ctttgtcgat
     4201 atctttatac agctccaggt gcttacggaa ctggtcgagg gtataccctt cttccgcgcc
     4261 cggcagaccg gcaatgatgt tacgggtcag acgcgctttg tcttcgtcgc tcatagtggc
     4321 gaagcgttca gcggcctgag caatttcttc ttcggtgtaa tccgcttccg cgcctggacg
     4381 cttcaggata tgcatttcga atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga
     4441 gccgtctggc agcacgtatt cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt
     4501 gtagcacacg gtgcgaatgc cgcactgcgc caggttgcgc agagtctgct gatagttagc
     4561 gatccactgt tcatagttgc cagtgtgggt tttgatatct tcgtgaattg gcacgctttc
     4621 tacgacagac cataccaggc ctgcgtcttc aacgatcgcc ttgcgtttga ggatctcttc
     4681 tacggaccat acttcgccgt tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc
     4741 cgcctgacgg acatcagcta gagaaaccgg atcgtttggg ccgtaccagc gccaggtctg
     4801 ttccatgatt tttcctcttt cacatagtgg gttccacagc aaagagacgt ggagccccgg
     4861 agttgtttag gtatccggat gaacaaccag accgcagccg tggcgttgca tcggatgaga
     4921 tggcgtataa gttctacctt ttggtaagac atgtcaaccc gaaatctgaa attggttaac
     4981 cacatcgcaa gaatttcact ctcacggttg ttgctgcaaa ttgcagcaaa gggtaatcga
     5041 tggttattga tcagaggatg tgacattcat cgcaacaatg gttgaccaat ttacataaca
     5101 tgtcgcccaa aataacaacg gttcaaccag gtaaggtaaa acgttatgca tgtgcttaac
     5161 attctctggg tggtattcgg cattggcctg atgctggtac tgaatttgaa gttcaaaatc
     5221 aattcaatgg tggctttgct agtggcggcg ctgtccgtcg ggatgctggc gggcatggat
     5281 ttgatgtcgc tgctgcacac aatgaaagcg ggcttcggca acacgctggg ggaactggct
     5341 atcatcgtgg tgttcggtgc ggtcatcggt aaattgatgg tcgactccgg cgcggcgcac
     5401 cagatagcgc atacgctgct ggcgcgtctc ggtctgcgct atgtacagct gtcggtaatt
     5461 atcatcggcc tgattttcgg tctggcgatg ttctatgaag tggcctttat catgttagcg
     5521 ccgctggtta ttgtcattgc cgccgaagct aaaatcccgt ttctgaaact ggcgatcccg
     5581 gcagtagcag ctgccactac cgcacattca ctgttcccac cgcagccggg tccggtggcg
     5641 ctggtgaatg cttatggcgc ggatatgggg atggtttata tctatggcgt actggtgacg
     5701 atcccaagtg tcatctgcgc aggtctgatc ctgccgaagt tcctcggcaa tcttgagcgc
     5761 ccaacgccat cattcctgaa agcagatcaa ccggtagata tgaataatct gccctctttc
     5821 ggcgtttcga ttctggtgcc gctgatcccg gcgatcatta tgatctctac caccatcgcc
     5881 aatatctggc tggtaaaaga tacccctgcc tgggaagtgg ttaactttat cggttcctcg
     5941 ccgattgcaa tgtttattgc gatggtggtt gcattcgtac tcttcggcac cgcgcgcggg
     6001 cacgatatgc agtgggtgat gaacgctttt gaaagcgcgg tgaagagcat tgcaatggtg
     6061 attctgatca tcggtgcggg tggcgtgctg aagcagacca tcatcgacac cggcattggt
     6121 gacaccatcg gcatgttgat gtcccacggc aatatctcgc cctacatcat ggcatggctg
     6181 atcaccgtac tgattcgtct ggcgacgggt cagggtgtgg tttcagcgat gaccgccgcc
     6241 gggattatca gtgctgcaat ccttgataca gcaactggtc agctggttgg cgtgaatccg
     6301 gcgctgctgg tgctagcgac ggctgcgggt tccaacaccc tcacccacat taatgatgcc
     6361 tcattctggc tgttcaaagg ttactttgac ctgtcagtaa aagacacgtt gaaaacctgg
     6421 ggtcagctgg agctggtcaa ctccgtggtt gggctgatta ttgtgttgat tatcagcatg
     6481 gtagcgtaaa agagcaccag agcctgccat tggcaggctc tggtcggatt gtcggcaaag
     6541 tgcgctttgt ttattcttat ctggcctaca aagggctaac gtgcgggttt gtagatagcg
     6601 tagcgcatca ggcaatattg cgtacctgca ttagcaatgt cctgtgattt ctttattgat
     6661 aaacaaaagt cacgccaata atcgattgca cattccctgc agtcacctgc cctccagtac
     6721 gtgcgtaatt tgccgttaat cccagactta ccgccgaagt ccctactgct cctaacgata
     6781 ccgtgttatt cgctggaata atcgtaccgt tgcgcgtcaa ctgtacgccg acgccctgcg
     6841 ctggtgaaaa cgaggcggta ttggtgaaaa tcgaattgcc cgcatctgcg gttgtgccgg
     6901 agaggtaata tcccaggttt tggcttttcg cacaataaac ggtaagagga attggcactg
     6961 aaccagggta gtccggcaga gtaacggtga catcacgagc agaaacatcg cagccgccag
     7021 tgggcaccac cacatcatta ttggcgtaaa tattccacac aaactgaaaa tcatcgctgt
     7081 tatagttgtt ggtctgtcgc aaaataagca cggcaattaa tgaaccagct ttaatcgcca
     7141 ccccgccagc actgctcaca ggcgtcaaat aaagcgccac cggccacggc ttatccgttc
     7201 tcgaattata aacaacccgc ggcgtttcgc tggtggtagg aaatggatag ctactgccac
     7261 tatattttac ggtcccggaa aaattagata acacgccgcc ataagccgag cctcgttgca
     7321 gtgtgacata gtctgtaatg gtttccggat aatcgttatg gcaaaagatt tgcgtcgaaa
     7381 gatccacgac caggttttgc cccacattca cggcaggcgc aaggtttaca taaacattag
     7441 cgctgccacc gccaataggg atagcggtgc cattggcggt tttacaggcg aatgaccagg
     7501 catttaccga ccagcccatc agcagtacag caaacagggt aataactcgt ttcattacaa
     7561 tcatctcttc gggttcagct gtaggtatag gtgatgctaa tcactgcctg aatggttccc
     7621 tgagtggctc cgccatttac cgtcaatgct ctgacctgta acgggaagtg cgctgattgt
     7681 gaggaatcat ccacctgaac tgttttggtt gcgccactat tcaatgtgtt gccactgtca
     7741 tcctgtagct ctaactggat gttttgcgcg gtcccctggt ttttataata tccggtactg
     7801 tcggctgccc cgctgaagct ggcagtgacc ctcgacgttc ccaccggaca attagtcaac
     7861 tcaagcgcaa catcatgcca ggccgatgcc gccccggcag acataagact gaaagaataa
     7921 agatcgccga gatctaccgt ggcattggtg gtggaaaccg tgcacggttt ggcgacgacc
     7981 ttaccgttca ccgtgatggt gacatcggct gcctgtatcg tcgcacttgc gagcgccaac
     8041 attgccgcca ataaataccc acgtttgcgc catttcatga gcatctccag ttactgatat
     8101 tcaagagtga aggtagccgt ggcattgata tgccccgcag tgacaggcac ctgtgtcgcc
     8161 attagccggg cgtaaaaatt cagcgtattt ggtttacccg gcgtcagggt cgtccacgaa
     8221 agcgcggacg atggggcatt aaggggtatc tgattttgtt gctcattcag aagctgtatt
     8281 cccagtcccg cagccgctga caccgtattt tcaagtgcaa gcaggttggc attgtggcta
     8341 tctgcaacac cggtaaaccc aacttttacg gcagaaacgg cattaccaca gggtgacagc
     8401 aaaatacgaa atggaacgac aggagtcgtc gcgccaatgt tgttaaattg cttcgccgcg
     8461 ttttccatca gatcaacagt aaaattggtt gattcagcgg ccacactaca gccgttatct
     8521 ctgacatagc cgcggatagt aatcgtgcta tccgcagcca aagcgcgact caccgccaac
     8581 cacaaaaaag cgcacagaag ataaaaaggt ttgtttctca tcacgccccc ttaacgacat
     8641 tcagctgata gctgggttaa taactgctgc tgactctctg gtggcagttg ataattggcg
     8701 acacagtgag cattttcctc ttctccccat ttcacctgaa cttttcccgc taaaggcatt
     8761 ccgctgaggt aaacctgacc attatccgca acaatgccgc tactctggct actctctgat
     8821 gtcaccatcg ccccaaacgg cagcggctta ttattgtggg ttagcgtcat gagcagtttt
     8881 atcccaacgc gcgctttaaa ctctgctcgc acgatcgccc cacgagtggg aacaacgtta
     8941 gcgaccgcgt tatctaaatc gacgttatca gccagggtat tggtatccag cgctactctg
     9001 ttttcccgat attcagtggc ataaggcagc acggcataac cgcgccagtc ggtacgcacc
     9061 cccgtctggt tttcgacttt tgcatctttt gcgccaggcg ctttaacaag caccaccgta
     9121 tcgtttaacg gctgccccag cgttacgcca ttggcatgag ccagtacccc accgctgact
     9181 ccgtaataga gctgcttaat atcatcgcta tggctgtaac cgatgttggc attgccgtaa
     9241 ccaccgcgat aattcagcgt ggcgtagcct gtacttccgc tattaccatc acctcccccg
     9301 gcgtaaccgg tttgcacgct atagctgagg ttgttgtctt ccagcaacgt accgtataca
     9361 ccggccagat tggtcatccg accgttgaga tcgtgtgaca tgctgtaact ggcactggca
     9421 tgtcgccact gagatttact gtcagaacgc agccagtggc tgaaaggaat attgacgtta
     9481 aggcagtatg actttc
//
LOCUS       DAESHI010000150         9401 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.152, whole genome shotgun sequence.
ACCESSION   DAESHI010000150 DAESHI010000000
VERSION     DAESHI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.152"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            205..1071
                     /gene="folD"
                     /locus_tag="KW011_003807"
     CDS             205..1071
                     /gene="folD"
                     /locus_tag="KW011_003807"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HBH7662039.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1073..1285
                     /gene="ybcJ"
                     /locus_tag="KW011_003808"
     CDS             1073..1285
                     /gene="ybcJ"
                     /locus_tag="KW011_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HBH7662040.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1393..1914
                     /locus_tag="KW011_003809"
     CDS             1393..1914
                     /locus_tag="KW011_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBH7662041.1"
                     /translation="MPTVITHAAVPLCIGLGLSSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSM
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSCYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="KW011_003810"
     CDS             complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="KW011_003810"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBH7662042.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3509..4003
                     /gene="ppiB"
                     /locus_tag="KW011_003811"
     CDS             3509..4003
                     /gene="ppiB"
                     /locus_tag="KW011_003811"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HBH7662043.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4006..4728
                     /gene="lpxH"
                     /locus_tag="KW011_003812"
     CDS             4006..4728
                     /gene="lpxH"
                     /locus_tag="KW011_003812"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HBH7662044.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4846..5355
                     /gene="purE"
                     /locus_tag="KW011_003813"
     CDS             4846..5355
                     /gene="purE"
                     /locus_tag="KW011_003813"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBH7662045.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5352..6419
                     /gene="purK"
                     /locus_tag="KW011_003814"
     CDS             5352..6419
                     /gene="purK"
                     /locus_tag="KW011_003814"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBH7662046.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6556..7449)
                     /locus_tag="KW011_003815"
     CDS             complement(6556..7449)
                     /locus_tag="KW011_003815"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBH7662047.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7446..8099)
                     /locus_tag="KW011_003816"
     CDS             complement(7446..8099)
                     /locus_tag="KW011_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HBH7662048.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8110..9369)
                     /locus_tag="KW011_003817"
     CDS             complement(8110..9369)
                     /locus_tag="KW011_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HBH7662049.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2092 a         2667 c         2493 g         2149 t
ORIGIN      
        1 tagacaagga aataagtcag ttccagattt acaacgccat catggacgaa aaatgaagct
       61 ttcaatctca gcgacggtgc gccccacctt cgcaagaggt cgcttcacgc gataaatctg
      121 aaacgaaacc tgacagcgcg ccccgcttct gacaaaatag gcgcatcccc ttcgacctac
      181 gtaacagatg gaatcctctc tctgatggca gcaaagatta ttgacggtaa aacgattgcg
      241 cagcaggtgc gctctgaagt tgctcaaaaa gttcaggcgc gtattgcagc cggactgcgg
      301 gcaccaggac tggccgttgt gctggtgggt agtaaccctg catcgcaaat ttatgtcgca
      361 agcaaacgca aggcttgtga agaagtcggg ttcgtctccc gctcttatga cctcccggaa
      421 accaccagcg aagcggagct gttggagctt atcgatgcgc tgaatgccga caacaccatc
      481 gatggcattc tggttcaact gccgttaccg gcgggtattg ataacgtcaa agtgctggaa
      541 cgtattcacc cggacaaaga cgtagacggt ttccatcctt ataacgtcgg tcgcctgtgc
      601 cagcgcgcgc cgcgtctgcg cccctgcacc cctcgcggca tcgtcacgct gcttgagcgt
      661 tacaacattg ataccttcgg cctcaacgcc gtggtgattg gcgcatcgaa tatcgtcggt
      721 cgtccaatga gcatggaact gttgctcgca ggttgcacca ctaccgtgac tcaccgcttc
      781 actaaaaatc tgcgtcacca cgtagaaaat gccgatctac tgatcgttgc cgtcggcaag
      841 ccgggcttta ttcctggtga ctggatcaaa gaaggcgcaa ttgtgattga tgtcggcatc
      901 aaccgtctgg aaaatggcaa agttgtgggc gacgtcgtgt ttgaagacgc ggctaaacgc
      961 gcctcataca ttacgcctgt tccgggcggc gttggcccga tgacggttgc cacgctgatt
     1021 gaaaacacgc tacaggcgtg cgttgaatat catgatccac agggtgagta acatggcgac
     1081 attttcttta ggtaaacatc cgcacgttga gctgtgcgac ttgctgaaac tggaaggctg
     1141 gagcgaaagc ggcgcgcagg cgaaaatcgc gattgccgaa ggccaggtga aagccgacgg
     1201 cgcggttgaa acgcgcaaac gctgcaaaat cgtcgccggt cagacagtga gttttgcagg
     1261 tcacagcgta caggttgttg cctgatccct ttcccggcct gtatcaacag gccggttctc
     1321 ttttcagact tttgccctct acgccccgtt catccatgct gatagcatcg acattttgcc
     1381 gaagcgacca tcatgccaac cgttattacg cacgccgccg ttcccctatg cattggttta
     1441 gggttgagtt cgaaagtcat ccctccgcgt ctgttatttg ccggaatcat cctggcgatg
     1501 ctgcccgacg ccgacgtatt gtcgtttaaa tttggcgttg cttacggcaa tgtttttggt
     1561 catcgcgggt ttacccattc gctggtgttt gcgtttgttg tcccgctgtt atgtgtgctg
     1621 attggacgcc gatggttcag ggctgggctg attcgctgct ggctgttttt aaccgtctcg
     1681 ttgctatcgc acagcatgct ggattcggta accactggcg gtaagggtgt tggctggctg
     1741 tggccgtggt cagatgaacg ctttttcgct ccctggcagg tgattaaagt cgcgccgttt
     1801 gcattgtctt gttacaccac gccgtacggg catcaggtga ttatttctga attgatgtgg
     1861 gtgtggttgc cggggatgct actgatggga atgttgtggt ggcgcagacg ataaccggat
     1921 gcgaaaactc gcatccggca atagcgcaat tacttacgac gccaggtggt cccttgcggg
     1981 ccatcttcca gaacgatccc catctcgtta agacggtcac gcgccgcatc tgccgccgcc
     2041 cagtctttcg ctttacgggc atccagacgc tgttgaatta acgcttcaat ctcagccact
     2101 tcgctgtcgt ctgcctgcgc gccgctttgc aggaacgctt ccggttcttg ctccagcagg
     2161 cctaatacag cggaaagttt acgcaggtga gatgccattg cattcgccgc tgccatatct
     2221 tctgctttca gacggtttac ttcacgcgcc atatcaaaca gtacggaata ggcttccggg
     2281 gtgttgaaat cgtcgtccat cgcttcaata aagcgcgctt caaacgcttc gccaccggca
     2341 ggagcaacgg ttttttctgt gccgcgcagg gcagtgtaga gacgctccag cgccgcacgc
     2401 gcctgcttca ggttctcttc gctgtagttc agctggctgc gatagtggcc cgacatcagg
     2461 aagtaacgca cggtttcagc atcgtagtat ttcagcacat cgcgcacggt aaagaagtta
     2521 cccagcgatt tggacatctt ctcgcggtca accatcacca ttccggagtg catccagtag
     2581 ttaacatact gaccatcatg ggcacaggtg gactgcgcga tttcattctc gtggtgcggg
     2641 aacatcaggt cagaaccgcc gccgtggata tcaaagtggt tacccagctg cttgcagttc
     2701 attgccgaac attcaatgtg ccagcctgga cggcccgcgc cccacggaga cggccagctc
     2761 ggttcgccct ctttcgacat cttccacaga acgaagtcca tcgggttgcg tttgtcgtcg
     2821 accacgtcaa cgcgcgcgcc tgcctgcagc tggtcgagat cctgacgcga cagcacacca
     2881 taagtcggat cggtcgggac gtcgaacatc acgtcgccgt tgtccgccgc ataagcgtga
     2941 cctttggcga tcagttgttc agtgagttca ataatttctg cgatatggtg cgtcgcgcgc
     3001 ggctccatat ccgggcgcag aatgttcaaa gcatcaaaat ctttgtgcat ttcggcgatc
     3061 atgcgatcca ccagcgccac aaagctttcg ccattttcat tggcacgttt gatgattttg
     3121 tcgtcgatat cggtaatgtt gcgcacatac ttcagtttat agccgaggaa acgcagatag
     3181 cgcgcaacca cgtcaaaagc aacaaaggta cgcccgtgac cgatatgaca gagatcgtaa
     3241 acggtgattc cacacacata catgccgact tccccggcgt gaataggctt aaattcctct
     3301 ttttggcgtg tcagagtatt gaagattttt agcatcgaag attccgttta gacatgtgtg
     3361 ggtaattgag ttgcgtataa tacccatatt tcccgcgcga atcagcatac attgcgtgat
     3421 gatccgatcg tgcggttatg ctataacacc accctatata tgacccgaac tgggttgaag
     3481 caccaatcaa acggaacagg atgcaaaaat ggttactttc cacaccaatc acggcgatat
     3541 tgtcatcaaa acttttgacg ataaagcacc tgaaacagtt aaaaacttcc tggactactg
     3601 ccgcgaaggt ttttacaaca acaccatttt ccaccgtgtt atcaacggct ttatgattca
     3661 gggcggcggt tttgaaccgg gcatgaaaca aaaagccacc aaagaaccga tcaaaaacga
     3721 agccaacaac ggtctgaaaa atacccgtgg tacgctggca atggcccgta ctcaggctcc
     3781 gcactctgca actgcacagt tcttcatcaa cgtggttgat aacgacttcc tgaacttctc
     3841 tggcgaaagc ctgcaaggtt ggggctactg cgtgtttgct gaagtggttg aaggcatgga
     3901 cgtggtagac aaaatcaaag gtgttgcaac cggtcgtagc ggtatgcacc aggacgtgcc
     3961 aaaagaagac gttatcattg aaagcgtgac cgttagcgag taatcgtggc gacactcttt
     4021 attgcagatc ttcatctctg cgtggaagaa ccggcgatca ccgccggttt tctgcgtttt
     4081 ttagcggggg aagctcgcaa ggccgacgcg ctgtatattc ttggcgatct gtttgaagcg
     4141 tggattggcg acgacgagcc caatccactc catcgccaga tggcggcagc gatcaaagcg
     4201 gtgtccgatt ctggcgttcc ctgttatttc attcatggca accgtgattt tctgctcggc
     4261 aaacgctttg cccgtgaaag tggcatgacg ttattgccgg aagaaaaggt gctcgaactt
     4321 tatggccgcc gggtgttgat tatgcatggc gacacgctgt gcaccgatga cgcgggttat
     4381 caggcttttc gcgccaaagt ccacaaaccc tggctgcaaa tgctattcct cgccctgccg
     4441 ttgtttgtgc gcaaacgcat tgccgcgcga atgcgagcga acagtaaaga agccaacagc
     4501 agtaaatcgc tggcgatcat ggacgttaac caaaacgcgg tagtcagtgc gatggaaaaa
     4561 catcaggtgc aatggctgat ccacggacat acccatcgcc cggcggtaca tgaacttatc
     4621 gccaaccagc aacctgcttt tcgcgtggta ctcggtgcct ggcatacgga aggttcgatg
     4681 gtgaaagtca cggcggatga cgttgagctg attcattttc cgttttaaaa aaaccgcaac
     4741 tttgctgatt tcacagccac gcaaccgttt tccttgctct ctttccgtgc tattctctgt
     4801 gccctctaaa gccgagagtt gtgcaccaca ggagttttaa gacgcatgtc ttcccgcaat
     4861 aatccggcgc gtgtcgccat cgtgatgggg tccaaaagcg actgggctac catgcagttc
     4921 gccgccgaaa tcttcgaaat cctgaatgtc ccgcaccacg ttgaagtggt ttctgctcac
     4981 cgcacccccg ataaactgtt cagcttcgcc gaaagcgccg aagagaacgg ttatcaggtg
     5041 attattgcgg gcgcaggtgg cgcagcgcac ctgccaggca tgattgccgc caaaacgctg
     5101 gtgccggtgc tgggcgtgcc agtacagagc gccgcactga gcggtgtcga tagcctctac
     5161 tccatcgtac aaatgccgcg cggcattccg gtgggtacgc tggcgattgg taaagctggc
     5221 gcggcaaacg cggcattact ggcagcacaa attctcgcga ctcacgataa agagctacac
     5281 cagcgtctga atgactggcg caaagcacaa actgatgaag tgctggaaaa cccggacccg
     5341 cgaggtacgg catgaaacag gtttgcgtcc tcggtaacgg gcagttaggc cgtatgctgc
     5401 gtcaggcagg cgaaccgtta ggcattgctg tctggccagt cgggctggac gctgaaccgg
     5461 cggcggtgcc ttttcaacaa agcgtgatta ccgctgagat cgaacgctgg ccggaaaccg
     5521 cattaacccg cgagctggcg cgccatccgg cctttgtgaa ccgcgatgtg ttcccgatta
     5581 ttgctgaccg tctgactcag aagcagcttt tcgataagct ccacctgccg accgcgccgt
     5641 ggcagttact tgccgatcgc agcgagtggc ctgcggtgtt tgatcgttta ggtgaactgg
     5701 cgattgttaa gcgtcgcact ggtggctatg acggtcgcgg tcaatggcgt ttacgcgcca
     5761 atgaaaccga acagttaccg gcagagtgtt acggcgaatg tattgtcgag cagggcatta
     5821 acttctctgg tgaagtgtcg ctggttggcg cgcgcggctt tgatggcagc accgtgtttt
     5881 atacgctgac gcataacctg catcaggacg gtattttgcg caccagcgtc gcttttccgc
     5941 aggccaacgc acagcagcag gcgcaagccg aagagatgct gtcggcgatt atgcaggagc
     6001 tgggctatgt gggcgtgatg gcgatggagt gttttgtcac cccgcaaggt ctgctgatca
     6061 acgaactggc tccgcgtgtg cataacagcg gtcactggac acaaaatggt gccagcatca
     6121 gccagtttga gctgcatctg cgggcgatta ccgatctgcc gttaccgcaa ccagtagtga
     6181 ataatccgtc ggtgatgatc aatctgattg gtagcgatgt gaattatgac tggctgaaat
     6241 tgccgctggt gcatctgcac tggtacgaca aagaagtccg tccggggcgt aaagtggggc
     6301 atctgaattt gaccgacagc gacacatcgc gtctgactgc gacgctggaa gccttaatcc
     6361 cgctgctgcc gccagagtat gccagcggcg tgatttgggc gcagagtaag ttcagttaag
     6421 caggtgctca tttgattgcc ggatgcggcg taaacgcctt atccggctta ccgctccggt
     6481 acccattgta ggcctgataa gacgcgtcaa gcgtcgcatc aggcatggtg caccgattgc
     6541 cggatgctct catcactaca gcgaaataca ggtccccgct tcgcccgcca gcgtctcttc
     6601 aattcgcgat aacgccccaa tccacgcggg tttaccacgg cttctgacat aaccactcac
     6661 cgccgttacc ttcggcccca tcgaaccatc ggctttggca aatggcgcta actcatccgg
     6721 tgtggcatgg cgaatggcac gttgctgcgg cgttccccag ttttcatata ccgcatcagc
     6781 atcggtgagg atcaccagtc catcggcatt aatctgctcg gcgagcaacg cagcggcgag
     6841 atctttatca atcactgctt cactccctgc cccatcttcc gtcacaggca cacctccgcc
     6901 gccactgcaa atcaccacat gcccctcttt gagcaacaac tcgatggctt cgctgtcgag
     6961 aattttacgc ggttgcggag acgccaccac ccggcgcaaa tatttaccat cacgtttcat
     7021 ctgccagccg taagccgctt ccagtgcctc ttgttcttct ggctgataaa ccggaccaat
     7081 aaatttctct ggctgcaaaa acgccggatc atcaggcgaa acctcaatgc gcgtcagcac
     7141 cgtcgtaaca ggcggcatct gcggctgtgc gctcaagctc tgcgccagca tatagccaat
     7201 catcccctgg ctttccgcca ccagcacatc cagcggatac ggttctacct ctttccacgc
     7261 cagattctgt aacgccagca gccccacctg cggcccgttg ccgtgaacaa tcgccagccg
     7321 ataagaacgg gccaggcgtg ccagcgcggg tacagcactg gcgatattgc gatattgatt
     7381 ttccgccgtc agcgcctcac cgcgctggag taaggcgttg cccccaagag ctacaaccag
     7441 tgttttcatg gttttccttg taataattgt tggccaagcc agaaccccag cagcgtatct
     7501 gcccctgacg tatgccccag cgccagcagc gaatcaatcg caacagcggt acgtttcggg
     7561 caactcagag catggacaaa gtgcaggagt ggcgaggcga aatatccttg cgcggcataa
     7621 cgtaaataac tgacgctcac cgctgtggta acgagttgaa gattgtcgga ataggcaaaa
     7681 aactggcgta actcagcagg cagcggatcg cctgccgcca acgccagtgg cccgaaaagc
     7741 ccggtttcct ccgcgcgttg catccatgca gctgcaagcg gttgaggatg cgcaggcggc
     7801 gtaatacgca gcaaacaata acgctgtggc tgtttaaccg tgaaacgccc gaggcgaatc
     7861 ccttgagcca caacctgtgg tcgttcattg ccgcataatc caccgcataa cgcatcgaat
     7921 tgcgcgcggc gaagcaccca tcctccgggg ccgaaaccac taccctgacg atgcagcgtt
     7981 aacagttcgc cgctttccgt catcaggtta atcgcccgtc gccacactcc cgctaaccgc
     8041 caggactggc gataattcgg tgcactacgg ctggcaagca gaggatggat gatcgtcatt
     8101 gcgcgttcct caaccaatcc ccatgctttc agccagtgct tccagcgcct gttcaaaaca
     8161 cgccagcggt gcccgcacag tgcctgcgcc aatctgcccg atccccgcct ctttatgggc
     8221 gataccggta ttgatgagtg gcgtaatgtc ggtttctacc acgcgacgaa tgtccagtcc
     8281 caggcacgcg ccctgaaaat cccagcctgg gatctgcaac tgcatattgc gctcaaggta
     8341 aatttccgcc atctcttcag ataccgctct ggccgcttcc atgccacccg caccgacaaa
     8401 gcgcgttacg ccaggcgctg cgatcattgc cgcacctccg ataccaaagg tttcggtaat
     8461 cgcgctatcg cccatatccg ggttcgcctg ctcctgcgag aagccggtga aaaacagacc
     8521 ttgcggagtg tttacaggcg cagtaaacca gcgttcgccc agcccgctta cccgaatccc
     8581 gaacatattg ccgttgcggg tcattgccgt gacgatgctg cctgcgcgga tcatcgcgcc
     8641 agcatccatc gccgccttgc agtaagccat cgcgaggttg aggaagaact gatcggtcac
     8701 gctgaggaaa tccatcactt cggcgatgtg ctgtttatca tgatcgaggc gagcaatttg
     8761 tggggccagc gcacgcatta acagtgcaga ggaagcaata ttgcgttgat ggaactcatc
     8821 gcccatcgta atgccctgcg ccatcatcgc cgtgagatcg ataccgcgct ccatgcgccc
     8881 cagcgccgcg cttaatactg gcattaacac atcgcgcatc cagcgatggc gagtcaggac
     8941 atcttcgccg taagcgccaa aacgcatcac tttgccgata ccttcgttga ggttgcagta
     9001 cgcccggttg ccgtcggtca cgttctcgac caccagcatc ggcatactgg cagaagtaat
     9061 accgcccatt ggaccgacgg cattcacatg gtgacaagga atgaagttca cttccccctg
     9121 ctccagtatt gccagcgcct gcgcttcatc tttcgcccag ccttcgaaca gacatgcgcc
     9181 cacgcacgcc cctttcatgg gtccggtcat ctcctgccag cgcattggcg gcccggcgtg
     9241 aagcagtgtt ttgccctcgt ttagttcgct gataagtgaa gaagccggtt gcacatccag
     9301 ccagtgtgga cgagcgcgac gaatttgttc gataaccgca gcattggctt gcgccactga
     9361 tgtaaacatg ggaacccctt attgcaaacg ttctaataaa c
//
LOCUS       DAESHI010000151         9345 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.153, whole genome shotgun sequence.
ACCESSION   DAESHI010000151 DAESHI010000000
VERSION     DAESHI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9345
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.153"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..96
                     /locus_tag="KW011_003818"
     CDS             <1..96
                     /locus_tag="KW011_003818"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBH7662050.1"
                     /translation="PAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(152..487)
                     /locus_tag="KW011_003819"
     CDS             complement(152..487)
                     /locus_tag="KW011_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HBH7662051.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(491..835)
                     /locus_tag="KW011_003820"
     CDS             complement(491..835)
                     /locus_tag="KW011_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HBH7662052.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(837..1010)
                     /locus_tag="KW011_003821"
     CDS             complement(837..1010)
                     /locus_tag="KW011_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662053.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            1111..1296
                     /locus_tag="KW011_003822"
     CDS             1111..1296
                     /locus_tag="KW011_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662054.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            1257..1376
                     /locus_tag="KW011_003823"
     CDS             1257..1376
                     /locus_tag="KW011_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBH7662055.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(1855..2121)
                     /gene="minE"
                     /locus_tag="KW011_003824"
     CDS             complement(1855..2121)
                     /gene="minE"
                     /locus_tag="KW011_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBH7662056.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2125..2937)
                     /gene="minD"
                     /locus_tag="KW011_003825"
     CDS             complement(2125..2937)
                     /gene="minD"
                     /locus_tag="KW011_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBH7662057.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2961..3656)
                     /gene="minC"
                     /locus_tag="KW011_003826"
     CDS             complement(2961..3656)
                     /gene="minC"
                     /locus_tag="KW011_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBH7662058.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4176..4544
                     /locus_tag="KW011_003827"
     CDS             4176..4544
                     /locus_tag="KW011_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HBH7662059.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4652..5053)
                     /locus_tag="KW011_003828"
     CDS             complement(4652..5053)
                     /locus_tag="KW011_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HBH7662060.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            5295..5588
                     /locus_tag="KW011_003829"
     CDS             5295..5588
                     /locus_tag="KW011_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HBH7662061.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5660..6319
                     /locus_tag="KW011_003830"
     CDS             5660..6319
                     /locus_tag="KW011_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBH7662062.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            6411..6857
                     /locus_tag="KW011_003831"
     CDS             6411..6857
                     /locus_tag="KW011_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HBH7662063.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(7064..7975)
                     /gene="hlyE"
                     /locus_tag="KW011_003832"
     CDS             complement(7064..7975)
                     /gene="hlyE"
                     /locus_tag="KW011_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HBH7662064.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKKHGKKTLFEVPEV"
     gene            8348..8767
                     /locus_tag="KW011_003833"
     CDS             8348..8767
                     /locus_tag="KW011_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBH7662065.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            8767..9252
                     /gene="umuC"
                     /locus_tag="KW011_003834"
                     /pseudo
     CDS             8767..9252
                     /gene="umuC"
                     /locus_tag="KW011_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
BASE COUNT         2400 a         2077 c         2176 g         2692 t
ORIGIN      
        1 ccggcggcca gtgtctgggg taatgtgggc gtgcagctgg gtgataatgg ctacaatgac
       61 accgcagtga tggtgggcct gaaatataag ttctgatccc cgttagctaa aataccgcgt
      121 cgaattcatc gacgcggtaa catattaaat attatttttt gccgatagca cgcatggtgt
      181 catcaattgc cgtgatcaac agcatttgcg ggtctttagc gcaaacctga ttcagttttt
      241 ctaccacttt ggcgctcagt tccggagatt gctcaatttt taaatcacgg ctggcaacgc
      301 tggcattacc catcaccgca acaatttctg caacctgtgc gctgtcagtt tttgccattt
      361 cgttggcttc tgcgcaagta atataggttt ctgacggcaa accgttttta atattgtagt
      421 cctgcgccca ggtcattggt gcgaaaacaa acaggcccgc cagtaaagca aattttttca
      481 tcatcattcc ttatttcatt ttacccagaa ttgcaccacc cgtaccgcca atcacggcac
      541 ctttaatcgc cccttcgagg ccattgccgg tcagaacgcc agtgacagca ccaacggcgg
      601 cacccacttt tgcaccttta cgtgcatttt taccgtcgcg gcctttttct gttactgcac
      661 caacaccagc gccaacagct gcacctttca gtacgccatt aacaccattg ccagtaagta
      721 atccaacgcc tgcgcctagc aatgcacctt tcgtggtgcg gttcatatcc gccatcgctg
      781 gcgtggagca gaacaatgct gagataagcc cgaaggcaag tatttttttc ttcaacttag
      841 atgtccggta ttaagtaagt tgcacacaca ataatttcgt cttcaattaa gatctgctta
      901 actaaagaac gctcgctatt attcagataa ttcaaaatga gcgtggctgt gatgatagga
      961 attatgtttt ttacgtgaat gagaataatc ttaaatgagg aataactcat tgattggcaa
     1021 tatttttatt caagaagtat cattgactgt taacgcaatg ttgtaaaggt aagataatct
     1081 gatttatcaa tattattgtg tgatttttat gtgagcagaa gatattcatc agcaacgatt
     1141 acattagtca ttttattttg ccgacggcct cattgtcgaa agataagcgt acgacagtat
     1201 tatctgaaaa gagtgatttt ttatccaact acacttcagc gcactgcgtt taaaaaatgc
     1261 ctctttctta tgcgggatat catcatttca tcatgatgtc tttggtgagc ggtgaacaca
     1321 atacacctgc gctgtctctt caggatgaat cccctggtct ggtgcctgcg ggctgatgtt
     1381 gcagcagagc ttaggtcact tagacgctac tatcatttat ccaatggcat ggaatcgaaa
     1441 tcagtcgata cccgcagtat atatcgtgaa ctggtcgttc tgaccatcaa cgattaaact
     1501 gcgcttcggc gcagttttcg tttacagggg gctaaaaggg aaaattctgg ggcaaaaaaa
     1561 gcccgccagt tacggcggga aacctcatcc tatgggagaa caatgaataa tgaaattgcg
     1621 gggttatcat cccccagtgt atccacacta acaataaggt tatttactca gccaggcata
     1681 aacattatgt tctgtgcgtg ggaacagcct taaggtgtaa agggggaggt ggaaatagca
     1741 atgaggagta tcagcaagaa tactcgccgc tttaccacaa cgtggatgag agggatgaaa
     1801 aactcaaggc agaaatgact ctgccttgaa gataaatgcg tttttacagc gggcttattt
     1861 cagctcttct gcttccggta aggtcacgtt cagctcaaga atagaaatat cgccatcttt
     1921 ttgctcaagc tgtacggtta ccatctcagg atcaatttgt acgtatttac aaatgacctc
     1981 aagaatatct ttacgcaact gcggcagata atgcggttct gcatcgctgc gacggcgttc
     2041 agcaacaata atctgcagcc gttcttttgc aatgttggct gtgtttttct tccgcgagag
     2101 aaagaaatcg agtaatgcca taacttatcc tccgaacaag cgtttgagga agcctttctt
     2161 ctcttcttca atgaagcgga aaggacgttc ttctcccaac agacgttcta cggtatctgc
     2221 gtaggcttta cccgcatcgg cgttaatgtc gagaatgacc ggttcacctt ggttagaggc
     2281 gcgcaatact gattgatcct ctgggatcac gccgacgagt ttgatgcgca ggatctccag
     2341 cacatcttcc atgctcagca tgtcacctct gcttacgcgg cctgggttat agcgcgttaa
     2401 cagcaggtgc tctttaatag gctcttcgcc attttctgcg cggcgtgatt tcgacgccag
     2461 aatgcctaaa atacggtcag agtcgcgtac tgaggagact tccgggttgg tggtaataat
     2521 ggcttcgtct gcaaaataga gtgccattaa cgcaccggtt tcaatccctg ccggggagtc
     2581 acaaacgata aattcaaaat ccatcgcttt cagatcatca agaactttgg cgaccccttc
     2641 acgggtgagg gcatctttat cgcgtgtttg cgatgccgga agaatataga gattttcagt
     2701 acgcttatct ttaattaacg cctgatttag cgttgcatcg ccctgaatga cgttgacgaa
     2761 atcgtaaacg acccggcgtt cgcaacccat aatcaggtcg agattacgca ggccgatatc
     2821 aaaatctatc acgacagttt tctttccctt ctgggccaaa ccagtggcga tggccgcgct
     2881 ggaggttgtc ttaccaaccc cccctttgcc cgaagtaaca acaataatgc gtgccataga
     2941 aattccttgt taaaaaggga tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac
     3001 tgcagtcgcg ccgctttgcc ataaaattct gctgggattt gatcactcag ccagtattca
     3061 cctgcgatgg acaccaattc cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca
     3121 ccacttgccc ctgccagcgc acgaccgcgc atcatgccat agacatgaat gttcccatcg
     3181 gcaatcaatt cggccccagc gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca
     3241 taaatacgct gaccggaacg caccggggta tctattaaac gcgtttttgt gaccggcgtg
     3301 gtattttgcg ctggagcctg tggtgcggga gctggacgtg gcgctttttc ctttccttcc
     3361 gtcaggatag gcagccccat tttttcaatt tcggctttaa gttgcgcgtc tttgcagcca
     3421 cttacgccaa taacccgcaa accggttgcc gaaaccgcct tatgcatcgc tgaccagttt
     3481 accgggtctt ccagtgcact gacgttgagt acaacggggg catgttttaa aaatgcgggg
     3541 gcctgagcga ttttgtcttc cagcgcctga tggataacct taggttctgc ctcatgcaga
     3601 tgaaccacag ataaagtgaa gctactgcct ttaagctcga ttggcgtgtt tgacatcctg
     3661 gccttactca attagctatt aatcatcgcc agcgcgcgat gatgttccga agactataag
     3721 gcatgttata gtctggatta tattgaggca agtcaccctc ccatttattc agagtaaaag
     3781 tctattctgt gataaatggc gctgattcat agcttaaaaa atacccttgt caatcaaccc
     3841 attgccgccg tacttttgat tcttcttatt tacgcttcct ttttccgcac cctaatcgtg
     3901 acagcatctc cagacctgtt taaatatcca gcattgaggc aagtgtattt tttcaccacg
     3961 ccaggctttc ttaactttcc attttttgca gcatcattaa agaacagata cccgttatct
     4021 taatatccgc cacagaattg ctctatcata gaaaccgcag aagacgtctg atacttctgt
     4081 caggtcatcg taactcgtcc aggtcagcgg ttttgattac ggattgatga cgaaatgagc
     4141 ataagtgttg tacggttgga ttatcaggat gtgctatgaa tatgatgcgt attatttata
     4201 tcggattgtc aggtgtgggg atgatgttct catcaatggc gtctggacat gacgctggcg
     4261 gacttcaatc tccggcgtgc ggcgttgttt gcgacccgta tatttgtgtg aactcagatg
     4321 gcatttcccc agagttaaca aggaaatatc tcggcgaaaa agccgctgaa aacttacaat
     4381 cattacaagg ctacgatccc agcgaattta cattcgctaa cggtgtattt tgcgatgtta
     4441 aagaaaaatt atgtcgtgat gatcgttatt ttggtgtaga tggtaagcgg agtggaaaaa
     4501 tcaatcaaac caccacaaag atgttattta tgtgtcgtga gtgatgcctt gtttacatta
     4561 tgttctctgc tattcgtcat taacctcttg agcgacagag aggagtgggg cgttaacgtc
     4621 tatgatgggg atagtccccg tcggttgaca tttacttaat ttgaatatcg acgttatatt
     4681 ttttagtttt gtttttacgg gcgtcgtttc gagtttgcag cactcgcagc tcatatttgc
     4741 cagaagcagg gagtgagtat tgtccgtgac tatcgagttc tggggaatat ctggataaat
     4801 caacagaatc atcgattcct ggcccgaaga gataagtatc agcgccttca ttagagatac
     4861 tcacatgcac tttctgacct tttttggcat agaaggtata tgtatcgtaa tcgtatccct
     4921 ttatttcgcc ggagtactga gcgctgctat ggcctttgct gaactcaaca ttgacatttt
     4981 tacccgcagc aaagctcgtg gaggttagca gtgcaagcag caatacagcc ttactgatgc
     5041 tcttgatttt cattggtagt ctcctgttca gaatgagtac gatgaactgt tataatataa
     5101 caatccctta cgggagaatt ccagtggctt ttctgcgttc ggtaaaatcc cggcgcggca
     5161 ttttttgccg gggggattac ttataaaggg gcatggactc aacaaaccgc agaaatctac
     5221 agctggttgg cttgtggcgc gggtttcatt gatgttagct tatgccaaaa cctggcatct
     5281 taaagagtaa aagtatgttt tgtgtgattt atcgaagcag caagcgtgac cagacctatt
     5341 tatatgtcga aaaaaaagac gatttttcgc gtgttcctga ggaactgatg aaaggttttg
     5401 gtcagcctca gttagcgatg attctgccgt tggatgggcg gaagaaactg gtcaatgctg
     5461 atattgaaaa ggttaaactg gcattaaccg agcaaggtta ctatttgcag ttaccgccac
     5521 cacctgaaga tttgctgaag caacatcttt ccgtcatggg gcagaaaaca gacgacacta
     5581 acaaataacc gatatccggc ggtggcatta tctttgtcgg cgcgggtttt catatccacg
     5641 ataaggtgag gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc tgctggatta
     5701 tccggtgagt aaagtagtct gtgttggcag taactatgcc aaacatatta aagagatggg
     5761 cagcgcagtg cccgaagagc cagtgctgtt tattaaacca gaaacggcac tgtgcgatct
     5821 gcggcagcca ttggcgatcc catccgattt cggttcagtt catcatgaag tcgaactggc
     5881 ggtgttgatt ggcgcgacgc tgcgtcaggc aacggaagag catgtccgca aagccattgc
     5941 cggttacggc gtggcgcttg atctgacatt gcgtgatgtt cagggaaaaa tgaagaaagc
     6001 cgggcagccg tgggaaaagg ctaaagcgtt tgataactct tgtccgcttt ccgggtttat
     6061 ccccgcggcg gaatttaccg gcgatccaca aaatacaacg ctgggcctga gcgtaaacgg
     6121 cgaacaacgc cagcaaggta cgactgcgga catgatccat aaaatcgttc cgttgatcgc
     6181 ttatatgagc aagtttttta ccctcaaggc cggtgacgtt gtgttgacag gcacgcctga
     6241 tggcgtcggc ccgttgcaaa gcggtgatga gctgacagtc actttcgatg ggcattcttt
     6301 gacaactcgc gttttgtaat actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc
     6361 tgtgccagac tggttataag gtgcgtttta acgtaatggc ggaacacctg atgagcgatg
     6421 tacctttctg gcaaagtaaa accctggacg aaatgagcga tgcggaatgg gagtcgttgt
     6481 gtgatggttg cggtcagtgt tgcctgcata aactgatgga tgaagacacc gacgaaatct
     6541 acttcactaa cgtcgcctgt cgccagctca atattaaaac ttgtcaatgt cggaactacg
     6601 aacgtcgttt cgagtttgaa cccgactgca ttaaattaac ccgtgaaaat ctgccaacat
     6661 tcgaatggct gccaatgatc tgcgcttatc gtttgctggc ggaaggtaaa gatttacctg
     6721 cgtggcatcc gctacttact ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc
     6781 gtcatatcgc agtgaaagaa tcagaagtga ttgactggca ggatcatatc ttaaataaac
     6841 ctgactgggc gcagtgataa atttaataga tgttgatggg ggctttctta atatacgtct
     6901 ggatgctcgc catttcgtag ccctgttttt gaaagcgctc tgtcataatg agagttaccc
     6961 gataccttat tatcaacagg taactctcaa tctttaaaaa acgtctgtgt ttttaaatgt
     7021 gataaacctt ataccttgag tacatggaga gaataatcgc tgatcagact tcaggtacct
     7081 caaagagtgt ctttttaccg tgttttttct gatactcatt acaggtgtta atcatttttt
     7141 tggccgcttc ttttagcaaa gaaagcatta aatcatcata atcaacgtag aatctggtgg
     7201 tttcagtttc cgtttttatc tctccgatgg cggctatttc ggtggttaat ttcaatttgg
     7261 cggcatcgat atctttattc gcttgtttaa ccgtgttaga cagggtggta aagaaactct
     7321 gcacagattt taacttgttc ttcaattctg ggatcagttt cccttcaact acgcccgcag
     7381 caatagaata ggaaatgatt aaaccaaatg gaccggcgac gacaccggct gcggcaccgg
     7441 catacgcttc cttcctgatt ttatctacct gtgactggaa atagctgctt ttttctgaaa
     7501 aatcattggt taactggcta tctaacgcca gcagtttccc ggaagcgttg ttgaaacttt
     7561 gtgagcttac cagcagggat ttttgcgctt cattcagctt cgtgatgccg tcatccagta
     7621 ccttaatgag aatgtctttc tgggcggatg ctttcttctc attgtactca tcaaatagca
     7681 aaatatacgc agcgagcaat tgcgtcgcaa caccacacca ttcatacact gtttgggttg
     7741 cttcaaaata cttatcctgg ctatccataa gtaaggtttt aatatcgccg actaaaacgg
     7801 aggctgcctg tgaatactcc tgtttaaagc gacttaactc ttttatggtt tcatcaaagg
     7861 tctgccaggg gatgacctga tcgagatatt tattataaag atctaatgct ccatctgcgg
     7921 tttcgattgc gtttttaact acttctaccg ttttatctgc aacgatttca gtcataatca
     7981 ttcgcctctt taaatatata aattgtaatg aaactcctgt tttacaacta ttaataaatt
     8041 ttacttcatc taattcatag ttagccgggc gggatgcgtc aatgtcttta tttctattaa
     8101 tatgataaat atcaaacaat gtttaatgtc attatggcga atgcttctat tctatttttt
     8161 tgccgggtga tatttttcat ttctgctgga tgagcgtcgt cgccagaagg ccacgtgagc
     8221 acaggataag agaacgaaaa atcagcagtc tatgcagcga caaatattga tagcctgaat
     8281 cagtattgat ctgctggcaa gaacagatta ctgtatataa aaacagtata acttcaggca
     8341 gattattatg ttgtttatca agcctgcgga tctccgcgaa attgtgactt ttccgctatt
     8401 tagcgatctt gttcagtgtg gctttccttc accggcagca gattacgtcg aacagcgcat
     8461 cgatctgaat caactgttga tccagcatcc cagcgcgact tacttcgtca aagcaagtgg
     8521 tgattctatg attgatggtg gaattagtga cggtgattta ctgattgtcg atagcgctat
     8581 taccgccagc catggtgata ttgtcatcgc tgctgttgac ggcgagttta cggtgaaaaa
     8641 attgcaacta cgcccgacgg tacagcttat tcccatgaac agtgcgtact cgcccattac
     8701 catcagtagc gaagatacgc tggatgtctt tggtgtggtg atccacgtcg ttaaggcgat
     8761 gcgctgatgt ttgctctctg tgatgtaaac gcgttttatg ccagctgtga gacggtgttt
     8821 cgccctgatt tatggggtaa accggtggtt gtgctatcga ataatgacgg ttgcgttatc
     8881 gcccgaaacg ctgaggcaaa ggcgctgggt gttaaaatgg gcgatccctg gttcaaacaa
     8941 aaagatctgt ttcgtcgctg tggtgtggtt tgctttagca gcaattatga gctttacgca
     9001 gacatgagca atcgggtgat gtcgacgctg gaagaactat cgccccgcgt cgagatttac
     9061 agtattgatg aggcattctg cgatctgaca ggtgtgcgta attgtcgcga tctgactgat
     9121 tttggcagag aaattcgcgc aacggtgcta caacgtaccc atcttactgt tggtatgggg
     9181 atcgcccaga ccaaaacgct ggctaagctt gccaatcatg cggcaaaaaa atggcagcgg
     9241 cagacggggg gtgcccctat taagttgtca agcatgttat gacccctgcg gggtataaaa
     9301 agtcccgtcg cgcatcatgg cgaacagaac gtcgcagcgt cgtct
//
LOCUS       DAESHI010000152         9217 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.154, whole genome shotgun sequence.
ACCESSION   DAESHI010000152 DAESHI010000000
VERSION     DAESHI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9217
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.154"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            73..732
                     /locus_tag="KW011_003835"
     CDS             73..732
                     /locus_tag="KW011_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HBH7662066.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            836..1810
                     /gene="ghrB"
                     /locus_tag="KW011_003836"
     CDS             836..1810
                     /gene="ghrB"
                     /locus_tag="KW011_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HBH7662067.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(1860..2570)
                     /locus_tag="KW011_003837"
     CDS             complement(1860..2570)
                     /locus_tag="KW011_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HBH7662068.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            3004..3294
                     /locus_tag="KW011_003838"
     CDS             3004..3294
                     /locus_tag="KW011_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HBH7662069.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            3575..3787
                     /gene="cspA"
                     /locus_tag="KW011_003839"
     CDS             3575..3787
                     /gene="cspA"
                     /locus_tag="KW011_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HBH7662070.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="KW011_003840"
     CDS             complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="KW011_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HBH7662071.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
     gene            complement(4453..6522)
                     /gene="glyS"
                     /locus_tag="KW011_003841"
     CDS             complement(4453..6522)
                     /gene="glyS"
                     /locus_tag="KW011_003841"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBH7662072.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(6532..7443)
                     /gene="glyQ"
                     /locus_tag="KW011_003842"
     CDS             complement(6532..7443)
                     /gene="glyQ"
                     /locus_tag="KW011_003842"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBH7662073.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(7538..7837)
                     /locus_tag="KW011_003843"
     CDS             complement(7538..7837)
                     /locus_tag="KW011_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HBH7662074.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            8012..9007
                     /gene="wecH"
                     /locus_tag="KW011_003844"
     CDS             8012..9007
                     /gene="wecH"
                     /locus_tag="KW011_003844"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HBH7662075.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(9049..>9217)
                     /locus_tag="KW011_003845"
     CDS             complement(9049..>9217)
                     /locus_tag="KW011_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662076.1"
                     /translation="MVGLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPPK
                     ETQVTQEEYSE"
BASE COUNT         2287 a         2331 c         2268 g         2331 t
ORIGIN      
        1 tcatgttcac gcggcataat ctcccgccac ggaacccgtg gcaaagaata aaaaggttat
       61 taaggattaa caatgaagaa acgtgtttat cttattgccg ccgtagtgag tggcgctctg
      121 gcggtatctg gctgcacaac taacccttac accggcgaac gcgaagcagg caaatctgct
      181 atcggcgcag gtctgggctc tctcgtgggc gcgggtattg gtgcgctctc ttcttcgaag
      241 aaagatcgcg gtaaaggcgc gctgattggc gcagcagcag gcgcagctct gggcggcggc
      301 gtgggttatt acatggatgt gcaggaagcg aagctgcgtg acaaaatgcg cggcactggt
      361 gttagcgtaa cccgcagcgg ggataacatt atcctcaata tgccgaacaa tgtgaccttc
      421 gacagcagca gcgcgaccct gaaaccggca ggcgctaaca ccctgaccgg cgtggcaatg
      481 gtgctgacag agtatccgaa aacggcggtt aacgtgattg gttataccga cagcacgggg
      541 ggtcacgacc tgaacatgcg tctctcccag cagcgtgcgg attccgttgc cagcgcgttg
      601 atcacccagg gcgtggacgc cagccgcatc cgtactcagg gccttggccc ggctaaccca
      661 atcgccagca acagcaccgc agaaggtaag gcgcaaaacc gccgtgtaga aattacctta
      721 agcccgctgt aatccctttc atgccaggtg atgcagaaat cacctggcat tcttcagttt
      781 tccttcatca tatttcaggc taaggtgatc gccttatcag tgaatggaga gaagcatgaa
      841 gccgtccgtt atcctctaca aagccttacc tgatgattta ctgcaacgcc tgcaagagca
      901 tttcaccgtt caccaggtgg caaacctcag cccacaaacc gtcgaacaaa atgcagcaat
      961 ttttgccgaa gctgaaggtt tactgggttc aaacgagaat gttgatgccg cattgctgga
     1021 aaaaatgccg aaactgcatg ccacatcaac gatctccgtc ggctatgaca attttgatgt
     1081 cgatgcgctt accgcccgaa aaattctgct gatgcacacg ccaaccgtat taacagaaac
     1141 cgtcgccgat acgctgatgg cgctggtgtt gtctaccgct cgtcgggttg tggaggtagc
     1201 agaacgggta aaagcaggcg aatggaccgc gagcataggc ccggactggt acggcactga
     1261 cgttcaccat aaaacactgg gcattgtcgg gatgggacgg atcggtatgg cgctggcaca
     1321 acgtgcgcac tttggcttca acatgcccat cctctataac gcgcgccgcc accataaaga
     1381 agcagaagaa cgcttcaacg cccgctactg cgatttggat acactgttac aagagtcaga
     1441 tttcgtttgc ctgatcctgc cgttaactga tgagacgcat catctgtttg gcgcagaaca
     1501 attcgccaaa atgaaatcct ccgccatttt cattaatgcc ggacgtggcc cggtggttga
     1561 cgaaaatgca ctgatcgcag cattacagaa aggggaaatt cacgccgccg ggcttgatgt
     1621 cttcgaacaa gagccactgt ccgtagattc gccgttgctc tcaatggcca acgtcgtcgc
     1681 agtaccgcat attggatctg ccacccatga gacgcgttat ggcatggccg cctgtgccgt
     1741 ggataattta attgatgcgt tacaaggaaa ggttgagaag aactgtgtga atcctcacgt
     1801 cgcggactaa gccgcgactg cgtgaagtaa agcccgataa tcgctcgggc ttttactctt
     1861 tattgggttg cagtaactgc tgtagtccag gcctgattaa acgcctgatg ttgtgccggt
     1921 aatggcgcaa tcagtttgtt atattcactt gcctgctgtg aagtcgggaa ctggatgcca
     1981 tttgcgacaa agctcacctg agtaccttgc tgagcaatgt agtctccaac catcaccagt
     2041 tgttgcgtga aagtttgtgc cgccggaatc aacggttgca atgcatcagc tggcgtcgtt
     2101 accactttgg tgaatgcctg atcaaatacc ggtttcaggt catcgctctg ttttagcgca
     2161 gagtgcgcag catcggcctg caacttcgca ttttgcagtt gttgcgccag cacacccagc
     2221 gaaccattca tttcacgcag cggaccgctt tgcgtaacgt aatcctgcgg tacgcgaatt
     2281 gcgttaacgc tgtctacgac cgggcgcaga ccagaatcca tcgcctgatt cacctgctga
     2341 gaataaccat acagaatcgc gtaatcagag acaaaaggac caaactgttt tttctgatcg
     2401 gcagtcaggg ttggtagacg ttcaccgcta cgcatcactg tattctgcag gaagtcgata
     2461 aacgctttac gctgatcgcc ttctttatca aaacacccac tcaggctaac taccattaat
     2521 aacgccgcaa gaggcgcaaa ccagcgagag caggactttc ctgtcgccat tttcttactc
     2581 ctttcaccca aaaaaagcgc acaacgacac acgcgtgtct gacgtgacaa ggatagtcca
     2641 ggacaggctt gcaggatacc ctttctacgt aatcttcctg gtaaaatcat ggagaaaatg
     2701 aaacaaggtt aatttgttgt ttcattgtta aaaaatgata gtgccgttgc cttttttgta
     2761 gccagagatt tacgccttcg cagaaggtta gattttcgaa atctttacaa aacaggagtg
     2821 gtaagagata tatcggcatt tatcagatcg ttaggtggct ataagtcacg ctaaatgaca
     2881 ggctgaatcg aatcatagcc agagcatgcc ctgacttcac cccgctgtgt ctgcttttcc
     2941 cgactattct taatgagctt cgatgcaatt cacgatcccg cagtgtgatt tgaggagttt
     3001 tcaatggaat ataaagatcc aatgcatgag ctgttgagca gcctggaaca gattgttttt
     3061 aaagatgaaa cgcagaaaat taccctgacg cacagaacaa cgtcctgtac cgaaattgag
     3121 cagttacgaa aagggacagg gttaaaaatc gatgatttcg cccgggtttt gggcgtatca
     3181 gtcgccatgg taaaggaatg ggaatccaga cgcgtgaagc cttcaagtgc cgaactaaaa
     3241 ttgatgcgtt tgattcaagc caacccggca ttaagtaagc agttgatgga atagactttt
     3301 atccacttta ttgctgttta cggtcctgat gacaggaccg ttttccaacc gattaatcat
     3361 aaatatgaaa aataattgtt gcatcacccg ccaatgcgtg gcttaatgca catcaacggt
     3421 ttgacgtaca gaccattaaa gcagtgtagt aaggcaagtc ccttcaagag ttatcgttga
     3481 tacccctcgt agtgcacatt cctttaacgc ttcaaaatct gtaaagcacg ccatatcgcc
     3541 gaaaggcaca cttaattatt aaaggtaata cactatgtcc ggtaaaatga ctggtatcgt
     3601 aaaatggttc aacgctgaca aaggcttcgg cttcatcact cctgacgatg gctctaaaga
     3661 tgtgttcgta cacttctctg ctatccagaa cgatggttac aaatctctgg acgaaggtca
     3721 gaaagtgtcc ttcaccatcg aaagcggcgc taaaggcccg gcagctggta acgtaaccag
     3781 cctgtaatct ctgcttaaaa gcacagaatc taagatccct gccatttggc ggggattttt
     3841 ttatttgttt ttcaggaaat aaataatcga tcgcgtaata aaatttagta ttatttttgt
     3901 gaagaataaa tttgggtgca atgagaatgc gcaacgccgt aagaaaggcg ggaataactt
     3961 cccgccaaag actcttactc tttcaaattg caggctaaaa atgccgccag ctcataactc
     4021 tcctgtttaa tatgcaattc acacagtgaa tctcttatca tccaggtgaa aaataaaagc
     4081 gtgaaacaaa tcactattaa agaaagtaat ctatatttct gcggcattcc cagcctcctg
     4141 tgttgatttc caacgagtaa gaggctaacc ttatggtgtt caggcatagg gtagcctcac
     4201 tttgatttat agtcaggtgg ggcttttctc tgtctgcctt tcggtgaata cctgagacaa
     4261 acagtctcaa gcacccgtgg ctattctagc ttaataagtt tgtttcttct ccttgatata
     4321 atcctaaaaa aacctcataa aattaatata tgagataatc tttattcaac agaagattat
     4381 taaaggttgc tgtattattt agcgataata aaaaaagcct gccagatggc aggcttttta
     4441 ataacggcgt tattactgca acagcgaaat atccgcaacg cgcaggaaca gttcgcgcag
     4501 tttctccagc atggtcagac ggttgagacg caattctttg tcatcaacca tgaccatcac
     4561 tttatcgaag aaagcatcaa ccggctcacg cagctctgcc agttcgacca gcgcatcctg
     4621 gtaacgacct tccgcaaagt acggctccag cttgtcacgt agcaccacaa cctgcatcgc
     4681 cagtttaatt tcttccggct ctttcagggt agaggcattc acgcggtcgc tcagcacttc
     4741 gtcagatttc gccagaatgt tagatacacg tttgttcgcc gccgccagtg cagcagctgc
     4801 atccagggta cggaaatgcg ataccgcttt catacgggca tcgaaatcag ccggacgagt
     4861 cggacgacgc gccagtaccg cctggatggt gtcaacggtg taaccttcgt cctgatacca
     4921 ggcgcggaag cgaccgagca taaagtcgat aacatcatca actacgttgg cgttagtcag
     4981 cttatcgcca tacagacgca ctgcttcttc ggtcagcgtt tgcagatcaa ggttgaggtt
     5041 cttctcaacg ataattcgca gcacgccaag tgcggcacga cgcagcgcaa acgggtcttt
     5101 gtcgcctttc ggatgctgac cgataccgaa gatacccgcc agagtatcca tcttatcagc
     5161 aatcgccagc gcacaagcta ccgggttgga cggcaggtca tcaccggcaa agcgcggctg
     5221 atactgctca ttcagcgcga cggctacgtc ttccgcttcg ccatcgtgac gcgcgtagtg
     5281 catccccata acgccttggg tgtcggtgaa ctcgaagacc atgttggtca tcaggtcaca
     5341 cttagacagc aggcccgcac gggttgcgtg gttaacgtca gcgccaatct gttcagcaat
     5401 ccagccagcc agcgcctgga tgcggtcagt tttgtcacgc agcgtaccca gctgttgctg
     5461 gaacagcacg gtttgcaggc gcggcaggtt atcttccaga cgttttttac ggtcggtatt
     5521 gaagaagaac tcggcatccg ccagacgtgg acgaacgact ttctcgttac cagagataat
     5581 ttgctgcgga tctttcgatt cgatgttggc aacgaagatg aagttcggca gcagtttgcc
     5641 atcgttcgca tacaccggga agtatttctg gtcacctttc atggtgtaaa ccagggcttc
     5701 agccggcacc gcgaggaatt tctcttcgaa ttttgcggtc agaacgaccg gccactccac
     5761 cagcgaagcc acttcttcca gcaggctttc gcttaagtca gcgttaccgc caatcttacg
     5821 cgctgcttct tcggcatcgg ctttaatctt cgctttacgt tcttcgtaat cggcgatgac
     5881 tttcccgcgc tcacgcagaa tttccggata ctgatcggcg ttgtcgatgg tgaattccgg
     5941 ttcgcccata aagcggtggc cgcgaatcac gcgatcggac tgaatgccca gaatggttgc
     6001 cggaatgact ttgtcgccca gcagcagggt cacggtgtgc accggacgca cgaagtgcac
     6061 gtcgcttgcg ccccaacgca tcagtttcgg gatcggcagt ttcgccagcg aagtcgcaac
     6121 catattcggc agtagtgctt cagtgctttc gcccttcaca tgggcgcgat acagcagcca
     6181 ttcgccttta tcggtagtca gacgctcagc ctggtcaacg gtaataccgc aaccacgcgc
     6241 ccaaccttct gccgctttgc tcggtttgcc ttcagcgtcg aacgcctggg caatcgccgg
     6301 gccgcgtttt tcgatttcac gatctggttg cgcttccgcc aggttagcta ctttcaacgc
     6361 cagacgacgc ggagcagcaa accattgaac ggtgccgtgt gcgaggccag cgttatccag
     6421 ctccgcagta aagttcgcag caaaggactc agccaggctg cgcagtgctt ttggtggcag
     6481 ctcttcagtg ccgatttcca ccagaaaagt tttctcagac atagccgcct cttacttatc
     6541 tttgttgcac atcgggaagc cgagggcttc acgggaagcg tagtatgctt ctgccactgc
     6601 tttggtcagg gtgcgaatgc gcagaatgta gcgctgacgc tcagtgacgg agatggcttt
     6661 acgcgcatcc agcaggttga agctgtgggc ggctttcaga atacgctcgt aggctggcag
     6721 cggcagcgga ttttccagcg ccagcagctg ctgcgcttct ttctcgtact gctcgaagca
     6781 ggtgaacagg aaatccacat ccgcgtattc gaagttgtaa gtggactgct ccacttcgtt
     6841 ctgatggaac acgtcgccgt aggtggtttt acccagcggg ccgtcgctcc agaccaggtc
     6901 gtaaacgctg tctacgccct gaatgtacat ggccagacgt tccagaccgt aggtgatctc
     6961 gccggtaacc ggtttacact ccagaccacc aacctgctgg aagtaagtga actgcgtcac
     7021 ttccatgccg tttagccaca cttcccagcc cagtccccag gcacccagag tcgggttttc
     7081 ccagttatct tccacgaaac ggatgtcgtg aatagtcggg tccatgccca gctctttcag
     7141 agaaccgagg tacagctcct gaatattgtc cggcgatggc ttaatgacca cctggaactg
     7201 atagtagtgc tgtaaacggt tggggttttc gccgtagcga ccatcggtcg ggcgacgaga
     7261 aggctgaaca taagcagccg ccatcggttc tggccccagc gcgcgcagac aggtcattgg
     7321 gtgagaggtt cccgcgccga cttccatgtc caatggttga acaatggtgc agccctggcg
     7381 agcccagtaa tcctgtaagg tcaggatcaa gccctggaag gtcctggtat caaacttttg
     7441 catattattt cgtgctggat acgtgtggat ttaaaggaag ggatcagtat acccgctgga
     7501 tggaagatat acagtacgaa acgggaaaaa gcagggctta acgcatggaa agatgcaaaa
     7561 aatggccatc tgcgtcaaaa gaacagacaa actgctcttt acggaaggta taaccgcgca
     7621 tttcatagct tccctggaac tgttcgaagg cggtgacgtc gattttttgc tcgccggtgt
     7681 tataacgttt tgccgcctga tccttgcata acgcttccat attcaacgaa cgcagaggat
     7741 caacctttac ccgttgtgct ttctgcacgg gtgacggtgt agaccaaccc actagcacca
     7801 taagcgccat tgccggaaag aaagcgttca tcatcattaa attaccgtct ggtttgcagg
     7861 tgagtcttat tatttatatc ggtatagtgg ctgatatctt acgaatttcc ttaagcctct
     7921 ataagctgta gtgataaaac tcagattatt ccagtgcgcc aaccaaatga tttatcggtc
     7981 agactttttt taaccagata cagagacaca gatgcagccc aaaatttact ggattgataa
     8041 cctgcgaggg atagcgtgtt taatggtggt gatgattcac accactacct ggtatgtgac
     8101 caatgctcat agtgttagcc ccgtcacatg ggatatcgcc aatgttctga attctgcctc
     8161 tcgtgtcagc gtgccgctat ttttcatgat ttccggctat ctcttttttg gcgaacgcag
     8221 cgcccagccg cgccatttct tgcgtatcgg cttatgtctg attttttata gcgcaatcgc
     8281 actgctctac attgcgctgt ttacctccat caatatggag ttagcgctga aaaacctgct
     8341 gcaaaagcca gtgttttacc acttgtggtt tttcttcgcg attgcggtga tttatctggt
     8401 ttcaccgctg attcaggtga agaacgtcgg cggaaaaatg ttgctggtgc taatggcggt
     8461 gattggcatt atcgctaacc caaacacagt gccgcagaaa attgacggtt ttgaatggct
     8521 gccaattaac ttatatatca atggcgatac tttttactac attctgtatg gcatgttggg
     8581 ccgcgctata gggatgatgg acacacagca taaagcactg tcgtgggtga gcgccgcgct
     8641 gtttgcgacg ggggttttta ttatctctcg cgggacatta tatgaattgc agtggcgcgg
     8701 aaattttgcc gatacctggt atctttactg tgggccgatg gtttttatct gcgcaatcgc
     8761 gctattgact ctggttaaaa acacgctgga tacgcgtacc attcgcggac ttggcttaat
     8821 ctcccgccat tcattgggta tatacggatt ccacgccttg attatccatg cgctgcgcac
     8881 ccggggaatt gagcttaaaa attggccaat actggatatt atttggatct tttgcgcgac
     8941 gttggcagcg agtttgttac tttctatgct ggtacaacga atcgacagaa acagattagt
     9001 gagttaagta aaagcccgtt cacattggac tgaccgggct tacgtgaatt attcgctgta
     9061 ttcttcctgg gtaacctgtg tttcttttgg cgggtttatt ccggcatcac gaatattctt
     9121 ctgcaccgct actgcaccaa acaagcttaa gaagaaagcc agtccataaa aacctttttc
     9181 actgagtaat aatgtcgcat tccacagacc taccatc
//
LOCUS       DAESHI010000153         9073 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.155, whole genome shotgun sequence.
ACCESSION   DAESHI010000153 DAESHI010000000
VERSION     DAESHI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9073
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.155"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(507..1274)
                     /gene="fecE"
                     /locus_tag="KW011_003846"
     CDS             complement(507..1274)
                     /gene="fecE"
                     /locus_tag="KW011_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HBH7662077.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(1275..2231)
                     /gene="fecD"
                     /locus_tag="KW011_003847"
     CDS             complement(1275..2231)
                     /gene="fecD"
                     /locus_tag="KW011_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HBH7662078.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(2228..3226)
                     /gene="fecC"
                     /locus_tag="KW011_003848"
     CDS             complement(2228..3226)
                     /gene="fecC"
                     /locus_tag="KW011_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HBH7662079.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(3223..4125)
                     /gene="fecB"
                     /locus_tag="KW011_003849"
     CDS             complement(3223..4125)
                     /gene="fecB"
                     /locus_tag="KW011_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HBH7662080.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4170..6494)
                     /gene="fecA"
                     /locus_tag="KW011_003850"
     CDS             complement(4170..6494)
                     /gene="fecA"
                     /locus_tag="KW011_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HBH7662081.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            complement(6581..7534)
                     /gene="fecR"
                     /locus_tag="KW011_003851"
     CDS             complement(6581..7534)
                     /gene="fecR"
                     /locus_tag="KW011_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HBH7662082.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(7531..8052)
                     /gene="fecI"
                     /locus_tag="KW011_003852"
     CDS             complement(7531..8052)
                     /gene="fecI"
                     /locus_tag="KW011_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HBH7662083.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIARD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            8344..9041
                     /locus_tag="KW011_003853"
                     /pseudo
     CDS             8344..9041
                     /locus_tag="KW011_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1988 a         2511 c         2585 g         1989 t
ORIGIN      
        1 acacttttta gaaaaatcga tggtcagaaa ctggattagc aattccgttc cagggttgct
       61 tttgatttac gttggcgtct gatcattgat ttatcctcaa aagcccaacc tcattggtaa
      121 tgaaccagct ccgtgaatgt ccgctctggc acagagcgaa attttttgat ctcccccccc
      181 tgaaatctaa acttagtcat gtcacgtttt tgggtttcta aaattttaac ttcgcgtttt
      241 tcgttgccgt aagggttata cagaaatgtc cgttaagcag agttcaaaat tgattgccgt
      301 gatcacgact ggtttgaaag ccgcgcccaa gcctgtacag ctctggtttg cgttgattat
      361 gaacctgtca gcctaaagca agcggatgga cgatgagtat tggtaatctt tcagagtccg
      421 gaaaagttca gccccagtct gaacaggctt gctggcgcca gtccagtttc attcagtcgt
      481 ggtttggttc ttacggcctg tgcaatctac ctcattaggc acatcggcct gccagatacc
      541 ggctcggggt gtatttccgc ttccacgctg aatactgttc tcagcaatcc tggggtcatc
      601 acctcttctg gtgtgccttg cgccataaca tgtccgtttg ccattaccac cagttgatcg
      661 cagtaccggc tagcctgatt aaggtcgtgc agcacagcga ccaccgtttt cccctgagtc
      721 cggagttcgc ccatcaaccg catcaggtcc acctggtgat tgatatcaag ataggtggtt
      781 ggctcatcaa gtaatacaac gggcgtattc tgggccagga ccattgccag aaatgcgcgc
      841 tggcgctgac cgccggaaag ctcggttaac cgacgaacgg caagatgatt gatccgggtc
      901 tggttcatgg cgacattaac tcgtgcattg tcttcagcgg agagacgccc ccagagtgac
      961 agccagggat tacgaccata cgaaaccagc tcctggactg tgatcccctc tggcgttaaa
     1021 tggtgctgag gcagcagcga aagcctgcgg gccaactggc gcgatgagag catatttatg
     1081 ggattatcgc cgagaaatac ggtgccagac tgcggcatta aaagccgcga aaaacagttt
     1141 aacagcgtcg atttcccgca accgttagga ccgatcaggg cggtgatctt ccccgttggc
     1201 agtgagagtg aaacgtcgtt aagtaccttg tctgtcccgt aattgaccgt cagattttca
     1261 gttcgtaaag tcatttatcg cattctcaca agcaaccaga caaaccacgg cgcaccgata
     1321 atggcggtca gcacgccaac cgggagctcc agtgggggat gaataattct cgccagcaga
     1381 tcggcaacca ccaacagcaa cgcacctgtc agggccgaaa caggcagcag tctgcggtga
     1441 cgtccaccgg tgatgctacg catcatatgc ggcaccacga gaccaataaa gctaatcggg
     1501 ccgcaggcgg ccacgccggt agatgtcatg gcgacagcta gtaacaaagc ccagaatcgg
     1561 gtatagggca ccgacacacc gagcgtggtg gcgcgcgcat cgccgagtgc aaggaggtcg
     1621 agatcgcggc aaaaactcag gctcagcggc agaaataaaa tcatcagcgg gatggcaatc
     1681 ttcacaaagc tccagtcacg gccccataag ctgccggtca gccacagcag ggcgttgttc
     1741 acatcctgcg ggcgcgagag catcagataa tccgtcaggc tggcccagca tgcagaaagc
     1801 gccacgccgg tgagtgccag cttcatcggc tggtgggtct ttgccagcat cttcagcaat
     1861 atcaaccccg ccatgccgcc cgcaaaggcc agcagcggca gcaccatcac gggcagtgac
     1921 ggcataagaa gtagagcccc cacagaggcc aggctggcgg catggttaac gccgagaata
     1981 tccggtgatg ccagagggtt gcgcacaatc ccctgtatca gcacgcccgc cacggcgagg
     2041 gctgcaccga caaacagtgc cagcagcaag cgcggcagtc ggtactccat caatacataa
     2101 taatgctcgc gtccggcctg ccagtcggtc agcagcgcgc gccacggcac ggggatcact
     2161 cccatatgga gtgataacag cgcacagccc gccagggcaa gggtgatgaa aataaccagc
     2221 gcaattttca tcctcgcctc ctcacaagcc agacaaagca agggctgcca atcagcgcca
     2281 gcactgcgcc tgcgggcaga tctccgggga aggccagcgc gcgtgcgagt acatctgcca
     2341 gcagcatcag cgtggccccc agcagcatgc tcactggcag tacgttgcgc tgatcgaagc
     2401 ctgcccagaa gcgcgccaga tgtggcacca gcagaccgat aaacgccacc ggacctgcca
     2461 cgctgacgca cgcaccaacc agaagcagca ctaacatatt gatgaccaaa cgtagcctcg
     2521 tcaggttcac tcccagcgta tgggcggtgc tgtcgctgag gttgagcagg ttcagttgat
     2581 tcgccagcag caacacgaca gggactgcag tgaccaccac cggcaagagc tgccagacat
     2641 cctgccagcg ggcgtgggac actccgccag ccagccagta aaagatgccg taagcatgat
     2701 cttcggccag cagcagggtg atgcgggtaa ggcccataca aaaggccgac agcgcgatac
     2761 ccgcgaggat cagtttgttt ctgtcatggg tatgacgaaa tccgcctcct gcggtcatga
     2821 ccagcagcca gctcacgccg cccccgcatg ccgcgatgaa cgacagagaa tagcctgcaa
     2881 tcggcgtcgg actcagcgcg ctggtaagcg ccatagccag cgccgcgccg ctgttaatgc
     2941 cgagcagtga aggagaggcc attgggttgt gggtcagggt ttgcagcagc gtgcccgcga
     3001 gcgccaggct tgcgccgatc agaacggcga ccaggcttcg tggcaaacga aggttttgca
     3061 ccagcgcttc tggtagcgtt ggcgtgtgtc caggcagcag ggcgcgggtt gcatctgctc
     3121 cggaaacagg aatggccgag tagcaaaaca gactcagcca gaaaataata ataagtgctg
     3181 caacgggaag cccccacagc agcaccgggt gttttatcgc ggtcatttca caacggtaag
     3241 cggctgatgg tggaagattt ttaccgtgtc agcggcaata cgctctgcag caaaaatacc
     3301 gcgcatccgc gcccaggtgt tactgtcgac cgaagcaacc tgctgcttct gcgcggcggt
     3361 taacatctgc cagagcggat cttgttgcca gcgtttaaca atgctctctt cgcgatagtg
     3421 ggcaaccagc agccaggcag gattgaccgc cagcagttgc tccaggccga tggacggcat
     3481 ggacgcaccc gccatcgcag cgggaacgtt cagccccaga gaggccagca cgctgccggt
     3541 ccaggtctcc tgagtatgca ggttgaattg ctgttcgcgt gatgtgccaa aggccacgcg
     3601 tgtccctttg ggaagctggc tggcccactg cgccatcctc tctttatgtt gttccagacg
     3661 tgcctgcatc tctcgctttt tacccaccat ttcgccgatg atagccgcag attgcaaatt
     3721 ttcagcgtag gtttcgttgc gggacttaag cagcagtacc gacgcgattt gctgcaaggc
     3781 gatgtaaacc cccgcatggc gactgctgtc ggcaatgatc aggtctggtt tcagagcggc
     3841 aatggcttcc aggctcggct gcgcgcgcgt tccgacggac tgccacggtt tcaggtgcgc
     3901 acgcacttcg ggcaggatgc gttttgcatc gttatcgtcg gcaataccga tcgggctgac
     3961 gtccacggcg gccagcgcat cggcgaacga gagttccagc accacaatcc gttgtggcgt
     4021 tttttcgagt gtaaacgtgc cgtgttcgtc ctgaaccgtg gcggcaaagg cgtggctgat
     4081 caccagcagc aggcctgcaa aaagaaaacg gataaatgcc aacataatca cattccagct
     4141 aaaagcccgg caagccgggc gttaacacat cagaacttca acgccccctg catatacagc
     4201 gtgcgcggct ggcctgcata gatgcctttg ttgttgtcgt cataagagcg gatgaagtag
     4261 tcctggtcga agatgttttt cacaccgaat gccaggttca gatctgccat ctgcgggcca
     4321 aagtcataca ccacgcgtgc gccccagagc atgaagccgg gaatgcggcc ggtactgccg
     4381 tcggcgctct ctttcaccgt attggcgtta tccgcaaact ggctggactg gaaatcgctg
     4441 ttcagattga acatccagtt tcctggcttg tagtccacgc ccagcgtgcc tttatgtttc
     4501 ggggagaatg gtaccagatt gccgtaggtg tcgcctttct cgcggatttc cgcgttcaca
     4561 tacgcatagc tggcgtagat ggaaacgtta tcaagcgttg gcgttagcgt acccagatcg
     4621 taacgtgcct gcgtttccag cccggtatgg cgcgttttgc cacgtgcagt gacggtgtcg
     4681 ttggtctggt tggagtcgta ctgattgtta aagttaatca ggaacagccc catttccgcc
     4741 gtcagcgcgc cgtcgtcgta gcgggtaccg agttcccagg ttcgcgcttt ttccggttcc
     4801 acattgccgc tttgcacagc cttgccaatc tggctgtact gtacggtgcc gaacgagcct
     4861 tcagtgtttg cataaagatt ccagctgtca gtcaggtgat agagcacgtt caacgccgga
     4921 agcggtgcgt tatagctcac ttcttcgtgc gtgcctgtga tggcgttgtt ctggtatgac
     4981 tcgatatgtt cgaaacgcat acccggcgtg atggtccagt tgccgatgtc gattttgtca
     5041 tccagatacc aggcgtgcgc ctcggtgccg gaacgcgtat cgcggtcgta agggcttgag
     5101 ccggacggca actgcccgct gctggtggcg gtgtagtaac gcatttcatg cgttgattca
     5161 ttcacatagc gatagcccac gcccacttcg tgcgcggaag ggccgatcat aaagatctgg
     5221 ctgtagcgtg gctcaatacc gcgcacccag tagttacgcg gcgagagggt gatgcgtttg
     5281 ccttgctcca ggtagccgct gcgcagggtt tgggtgtaga acccctgaat gttgaattta
     5341 tgctggctgt ctggctggaa ctggtagccc aggctcgcca gcttgcgacg accccagaag
     5401 cggtcatacg ggcgggtgga ttgccagcga tcggcgtcgt aatccgcgcg agacaggcca
     5461 ccgggcatgt cggcttcacc gtcgtaatat tgcagcaggc tgttgaaggt gtgcacctca
     5521 tccggcgcat atttgctttt cagcatcagg tcgtcgatgc gggtggcgct gtgctcgcgc
     5581 cagtcactgc cgcgcgtgcc ggagtagagc agcgcggtgc caaaaccgtt gtccgctgtg
     5641 ccgcccacca tcaggttgtg cgtctctttc gggttgtttt gtgaagaggt tgggctgagc
     5701 tgaccttcca cgcccgcctc gataccaaag tcctgcggaa tggcacgggt aacaaagttc
     5761 accacgccgc ccacgctctg cggtccgtaa cgcaccgcac caccaccgcg taccacgtca
     5821 atggcatcca tgttgccgag cgaaacggga gccagtgaaa gctgcggctg accgtaaggg
     5881 gcgaagggga cggggatgcc gtccatcagg acggtcgagc ggctggcgag gcgcgggttc
     5941 aggccccgga tgccaaagtt catcgccagg tcgtggctgc cggtgccgtt gttttccggc
     6001 gcgctgacgc cagggatgcg gttaagtacc tcacgcatgg tggttgcgcc ggttttggcg
     6061 aaatcctcac ggcggatcac gtcacgcgcg ccagcatgtt caaatacgtc gttttcacgc
     6121 gcatcaccca gccagtcgcc gaccacggtc agggcatctt cttttggtgc gggcgcgggc
     6181 tccagcgtcc agctgttatt tcccagcggt tttacctgca gtccgctgcc gtccagcagt
     6241 tgttgcaggc cgctctcgac gtcgtaatcg ccgtgcaggc cgttgctctg cttgccgcgc
     6301 gtcaggctgg cgtcaaccga gagggtaaat ccgctgtgtg cggcatactg attgagcgct
     6361 ttatcgagcg atcccggtgc gatattaacc tgtgcagcaa aagcggaaaa cgagagaccg
     6421 gccagcggca gcaggctcag gcgaatggcg ttaaccaaag gtgttgtttt acgaaaaacg
     6481 cgtaacggcg tcataccttc cccatcatca tttttgttgt gttcagctat gagtcgaacg
     6541 agaagggtaa aaaggacaat cgaaataaga attattttcc ttacagtggt gaaatgttta
     6601 tccagtaccg cgtaatagac tgaattttaa cgggaagcgt ttgcgcgata acgttcagga
     6661 tcgcatcggt atttttcagc gggaacgtcc cgctcaggcg cagcccggca acggcgggat
     6721 cgcagcgcag cacgccgttg cggtaacggg ttagcgtggc tatcacctca cccagcggtt
     6781 tatcgctgaa gctcaggatg tccttcgtcc agcttgtact ctcgtcatcc agcggtttca
     6841 ctgcgccaaa ctcagaggcg ctgaactgca ggctttcacc agcgttcacg atgcgttttt
     6901 gcgcgggggc actggcgaga agcacttcca cggcgtgctg ctgcacgtca agctgcgtga
     6961 aattatcctg ctggcggacg gtaaattctg tccctaaagc agtgagctgg ccctgacggg
     7021 tcaggacacg gaaggggcgt tgcagggcat ctttcgcggt agtaatggcg atttcaccgt
     7081 accagagccg gacggtgcgc tgatgcgcat caaaacgcat atccgccgcg ctttgggtat
     7141 tcagcgtgag cagggagcca tcttccagtt gctgacggct gactgtgcct ttggcggtgc
     7201 ggtaatctgc ccgcagacct tcgccggttt ccgactgcca gagctgccag cctccgccag
     7261 cgccgagcaa cagcagtaat cctttcatca cgtgacggcg ggtgaggcgg gtatcgtgca
     7321 acgcccggct ggcaacgtcg ccaggcacac cgccaagctg gttgcgcagg ttttcaacct
     7381 gctgccaggc ccactggtta tcctgatcct gttcatacca ctgttgccag cgcgcttcct
     7441 gttgtgggct gacgcgctcg ccgcttagca cggcatacca gtgggaagct gaacgcagcg
     7501 cctgacggcg ggaatcggtt aacaaaggat tcataaccca tactccagac ggaacagcag
     7561 gcagtgctcg acggctttcg ccacgtattt tttcacggag ctgatggaaa caccgagttt
     7621 gtgcgcaatc tcgctgtatg tcagaccatc cagttgcgaa agcagaaacg cttcacgtgt
     7681 tttgccgttt agcccgtcca gcatgctgtc gaggagttgt agggtctcga gttggctttc
     7741 gcgttcctca ggtgaaggcg ctcccccctc cggcataagc gccagcatct ccagatacgc
     7801 tttttccagc gcgtttcggc gaaacaggtc caccatcacg cgtttggcga tagtgcagag
     7861 gaaggagcga ggatcgcgga tcgtcgagag cgtttcgctg accattaccc gcaaaaaagt
     7921 gtcccgggca atgtcatctg catcaaaagc agactggagt ttgcgcgtca gccagctttt
     7981 caaccagccg tgatgtgtgc cataaagcga ctcgaatgtt aaggaagctg tggtagtggc
     8041 gcggtcagac atgcggagtg catcaaaagt taattatcac gtagccatat taatatgaga
     8101 atagttatca ttacaattgg aaataaaatt gtttccaata gacattttta acatgttgtt
     8161 tttctaagtg ttataaggta ggtataaaat gggatggagc ctctgcttct ggcatgtgtc
     8221 ggtcagaatg actcatgatg tggtctgcta ttattgacat cctcactgcc ctaaaggata
     8281 gggatttcgg taatgctgtc aacttactga tttagtgtat gatggtgatt ttaaggtgct
     8341 tgcgtggctt ccatttccat cagatgtcct tcctgctccg ctactgaagg cgtggtgcgt
     8401 aacggcaaaa gcactgccgg acatcagcgc tatctctgct ctcattgccg taaaacatgg
     8461 caactacagt tcacttacac cgcctctcag cccggtaagc accagaaaat cattgatatg
     8521 gccatgaatg gcgtcggatg tcgcgccagt gcacgcatta tgggcgttgg cctcaacacg
     8581 gttttacgtc acttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata caaccgggca
     8641 gtgatgtgat tgtctgcgct gaaatggacg aacagtgggg ctacgtcggt gctaaatcac
     8701 gtcagcgctg gctgttttac gcgtatgaca ggatacggag gacggttgtg gcgcacgtct
     8761 tcggtgaacg cactctggcc acactggagc gtcttctgag cctgctgtcg gcctttgagg
     8821 tcgtggtata gatgacggat ggctggccgc tgtatgaatc acgcctgaag ggaaagctgc
     8881 acgttatcag caagcgttac actcagcgca ttgagcgaca taatctgaat ctgagacaac
     8941 atctggcaag gctgggacgg aagtcactgt cgttctcaaa atcggtggag ctgcatgaca
     9001 aggtcatcgg gcattatctg aacataaaac actatcagta agttggagtc attacctcct
     9061 cccgaactca aaa
//
LOCUS       DAESHI010000154         8975 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.156, whole genome shotgun sequence.
ACCESSION   DAESHI010000154 DAESHI010000000
VERSION     DAESHI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8975
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.156"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            1..839
                     /locus_tag="KW011_003854"
                     /pseudo
     CDS             1..839
                     /locus_tag="KW011_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1453..1829)
                     /gene="rnpB"
                     /locus_tag="KW011_003855"
     ncRNA           complement(1453..1829)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KW011_003855"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1862..3007)
                     /gene="garK"
                     /locus_tag="KW011_003856"
     CDS             complement(1862..3007)
                     /gene="garK"
                     /locus_tag="KW011_003856"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HBH7662084.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(3104..3994)
                     /gene="garR"
                     /locus_tag="KW011_003857"
     CDS             complement(3104..3994)
                     /gene="garR"
                     /locus_tag="KW011_003857"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBH7662085.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(4024..4794)
                     /gene="garL"
                     /locus_tag="KW011_003858"
     CDS             complement(4024..4794)
                     /gene="garL"
                     /locus_tag="KW011_003858"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HBH7662086.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(4810..6144)
                     /gene="garP"
                     /locus_tag="KW011_003859"
     CDS             complement(4810..6144)
                     /gene="garP"
                     /locus_tag="KW011_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HBH7662087.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            6519..8090
                     /gene="garD"
                     /locus_tag="KW011_003860"
     CDS             6519..8090
                     /gene="garD"
                     /locus_tag="KW011_003860"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HBH7662088.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            8239..8644
                     /locus_tag="KW011_003861"
                     /pseudo
     CDS             8239..8644
                     /locus_tag="KW011_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
     gene            8644..>8975
                     /gene="yhaV"
                     /locus_tag="KW011_003862"
     CDS             8644..>8975
                     /gene="yhaV"
                     /locus_tag="KW011_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HBH7662089.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGW"
BASE COUNT         2403 a         2291 c         2085 g         2196 t
ORIGIN      
        1 aatatctaca ggcaatatca atgttaattg tccattaaaa aatatttaca atgaaatcag
       61 gaggttgaac gtaattttta gttgtgggac tggagatata gttgatctat cctctctgga
      121 cttacgtaat gtcgatttag attattacga ttttacagat aaacatatgg ctaatactat
      181 tttaaatcct tttaaattgg attcaacaaa ttttactaat gccaacatgt tccaggttaa
      241 ttttgttagt tcaacacaaa acgccacaat ctcctgggat tatttactaa aaataacgcc
      301 tgttttaata agcattagcg atatgtattc tgaagaaaaa atcaagtttg tcgaaagttg
      361 tttaaatgag cttggagaca ttaccgaaga acaattaaaa attatgagat ttgcaattat
      421 aaaatctata ccaagggcaa ctcttacaga taaattagaa aatgaattaa caaaagaaat
      481 atataaaagc tcatcgaaaa tcaacaattg cttgaacaga attaaattac cagagatgaa
      541 agaattctca tcagaaaaaa atatatgatt acatcgatat aatcattgaa gattatgaaa
      601 atatcaaaga aaatgcttat ctggtcgatc caaaaattaa ttacactata gatttaaaca
      661 tagaagactc tagctcagag gaattccttt ttgataatag cctcgagaaa gacgaaaatt
      721 cttcggacaa ttgctttgag gtcgaaaaat ataacgcata tgaagcatat aactcagaga
      781 atctatattt taccagagag gaatatacgt atgattacga ccttttaaat gcaatataga
      841 cataataact tcttacaaaa tctataatta ataccgtaga tattcattca tcttaccctg
      901 tgcaccaaaa gcaaaaactc actgacgcgg gtttgaaact gatggcaacc gcaaaaatgc
      961 ctgatgcgct acgcttatca ggcctacgcc atctctacaa tatattgaat ttgcgtgctt
     1021 ttgtaggcag gataaggcgt tcacgcagca tccggcatga ataaagcgca ctcaacaatc
     1081 tgaaacccgc cggaacggtt tattacgtac atcaggtgaa actgaccgat aagccgcttt
     1141 cttttgggta tagtgtcgtg gacagtcatt catctttctg cccctccaaa agcaaaaacc
     1201 cgccgaagcg ggtttttacg taaatcaggt gaaactgacc gataagccgc tttcttttgg
     1261 gtatagcgtc gtggacagtc attcatcttt ctgcccctcc aaaagcaaaa acccgccgaa
     1321 gcggtttttt acgtaaacca ggtgaaactg accgataagc cgctttcttt tgggtatagc
     1381 gtcgtggaca gtcattcatc tttccgctcc tccaaaagca aaaacccgcc gaagcgggtt
     1441 tttacgtaaa tcaggtgaaa ctgaccgata agccgggttc tgtcgtggac agtcattcat
     1501 ctaggccagc aatcgctcac tggctcaagc agcctacccg ggttcagtac gggccgtacc
     1561 ttatgaaccc ctatttggcc ttgctccggg tggagtttac cgtgccacgg actgttacca
     1621 gccgcgcggt gcgctcttac cgcacccttt cacccttacc tgatcccgct tgcgcgggcc
     1681 atcggcggtt tgctctctgt tgcactggtc gtgggtttcc cccccaggcg ttacctggca
     1741 ccctgcccta tggagcccgg actttcctcc cctccgcccg tctcccccga agaggacgac
     1801 gacgaagcgg cgactgtctg gtcagcttcg gcgcgcagta tagagggttt gcgcgccctt
     1861 gtcaccccgc gttgcgcatt ccaatcgcca gtgtcgcggc gatattacgt gaagcacggc
     1921 agatattgtc ataagccccg cggaatgctt cgtccaacgt acctatgctg gtcaatacgc
     1981 tgaagaccgc atcgatgcca tgctgatgta caacgccaac atcatcggtc aggctacccg
     2041 caatgccaat caccggttta tggtacttct tcgccacgtt tgcaacgcca atcggtacct
     2101 tgccgtgaat actctggctg tcaatacgcc cttcaccggt gatcaccagc gtacaatcgt
     2161 gaatatgttc ttccagattc agcgccgtag tgacgatttc aataccactt ttcagttccg
     2221 caccaagaaa cgccatcagc gccgcgccca taccgcccgc agctcctgcg ccggggacat
     2281 ctttcacatc aacatgcagc gcttttttaa tgacatcggc atagtgagag aggttattgt
     2341 ccagctcaac aatcatcgct tcactggctc ccttttgtgg gccaaagatg cgcgatgcgc
     2401 cgttatcgcc caccagcgga ttggtgacat cacaagcgac gcgaatgacg caatctttta
     2461 agcgcggatc gaggccggaa atatcaatat cattcagagt attaagacta ccgccgccaa
     2521 aaccaatttc attgccgttg gcgtcgcata atttcgcccc cagcgcctgt accatgcctg
     2581 cgccgccatc atttgtagcg ctgccgccaa tgccgataat aatgtttgtt gcactgctct
     2641 ccagcgcctg caggattaac tcgcctgtgc cgcgtgaagt ggttacgagt ggatcgcgtt
     2701 tttccgcagg taccagctcc agcccactgg ccgccgccat ttcaataaac gcggttttgc
     2761 catcgccgga gatcccccaa ctggcattca ctttctcgcc cagcggccct gtaacccagg
     2821 cgtgacgttc agccccctgg gtggctgcaa tcatcgcttc caccgttcct tcaccaccgt
     2881 cggcaaccgg aacagaaaca tactgtgcat caggaaaaat ttcccgaaat cctttttcta
     2941 tcgcctgcgc aacctcgctg gcagataaac tttctttata agagtctggg gcgattacga
     3001 ttttcatacc tatgcctgtt accacatgac gccggagggc gtttctctta ttcggcctag
     3061 attccaggcc cggattgcaa tacaccatcc gggcacgacg tcattaacga gtaacttcga
     3121 ctttcgccag tttttcgtag tagcacgcca gggcgctatg atccgccgtt cctaaaccat
     3181 ctgcacgcag tgcctgcatc atctccataa ccgcagctgt gagcagcagt tgtgcgccga
     3241 cgccgtgaga agtatccagc gcattcgcca gatccttaat atgcagatca atgcggaagc
     3301 ccggcttgaa gttgcggtcc atcaccatcg gcgctttggc atccagcacg gtactgcccg
     3361 ccagcccacc gcgaattgcc tgataaacca ggtccgggtt aacgcccgct ttggttgcca
     3421 gcgttaacgc ttctgacatc gcagcaatat tcagcgccac aatgacctga tttgccagtt
     3481 tggtgacgtt acctgcaccg atttccccgg tatgcaccac ggaacccgcc atcgctttca
     3541 tcaaatcata gtatttgtcg aaaatagcct tgtcgccgcc caccatcact gacagcgtac
     3601 cgtcgatggc tttcggttca ccgccgctca ccggagcatc cagcatatca atgcctttcg
     3661 ctttcagcgc ttcgctgatt tcacggcttg ccagcggtgc gatagaactc atatcgatca
     3721 ataccgtacc tggcttcgcg ccttcaataa tgccattctc acccagcgcc acctctttca
     3781 catgagggga gtttggcagc atggttatga tgacgtcgca ctgttcagcg atcgctttag
     3841 ccgtagacgc tgtttctgca cctgcagcaa tcacgtcagc aatagcttct gggttacggt
     3901 cagcaaccac cagcgagtaa cctgctttca gaaggttttt actcattggt ttacccataa
     3961 tccccaggcc aataaaacca actttcatag tcatatcaac catctctctt gttgcggtgg
     4021 tggttatttt ttaaaggtat cagccagttt ctgagtggca gagcggaaga cgccaagatc
     4081 gctgccgaca gccacaaacg tcgcgcccca ttccagataa cgacgcgcat cggcttcgac
     4141 cggcgcgaga ataccgctgg gtttgccgtg cgcgctggca cggttaaaaa tatgctgaat
     4201 tgctttttgt acatccggat gcgaggcatt gccgagatgg cccaatgccg cagccagatc
     4261 gctggggccg acgaagatac cgtctacacc ttcggtagcg gcaatagcat cgacgttatc
     4321 tacgccctgc tgactttcta tctggaccag aatagtaatg ttcttgttcg actgagcgaa
     4381 ataatccgcc acggtgccaa acatattggc gcggtgagaa acggagacgc cgcgaatgcc
     4441 ttccggcggg taacgagttg atgccaccgc ctgctctgct tcctcttttg tttctacaaa
     4501 aggaatcagg aagttataga aaccgatatc cagaagacgc ttaataatta ccggctcgtt
     4561 ggtcggcact cgcactactg gcgcgctggc gctgcctttc agggccatta actgcggaat
     4621 aaacgtggaa atatcgtttg gcgcatgttc gccatccagc accagccagt caaacccagc
     4681 caaaccaaga acttcagtgc taatcgggtt agagagtgct gaccagcaac caatttgtac
     4741 ctgtttcgca gccagtgcgg ctttgaattt attcgggaaa acatcgttat tcatcgctta
     4801 tacctttgct tatttctgca attccatacg tttaatgtcg ccaactacga agaggtagca
     4861 gaccatcgcc atcagcgctg aacatcccac gaaaaccagt gctgcattga aggagtgcag
     4921 ttcacttacc aggtagccaa tcaccagtgg agtgacaatg gaggcaacat tgccaaagac
     4981 gttaaagacg ccgccacaga ggccaacaat ctctttcggc gcggtgtcag aaatcaccgg
     5041 ccagcccaga gcaccaaatc ctttgccaaa aaaagccagc gccatcagca tgaccaccag
     5101 cgtggtgttg ttggtgtagt tacataagat gatggtggaa accagcaaca ttcccagcac
     5161 aatcggtagc ttacgtgcca gggtcaggga taaaccgcgt ttgatcagat aatccgagaa
     5221 gacacctccc agcacgccgc ccgcaaaacc acacagtgcc ggaatcgagg cgaccagacc
     5281 cactttcaga atcgacatgc ctttttcctg caccagataa atcgggaacc aggtgaggaa
     5341 gaaccaggtg atggtgttga taaaatattg tccgaaaaat acgcccaaca tcatgcggtt
     5401 agagagcaat tgcttgatgt aatgcagttt gggaccgctt gctgccgcgc tgcccggctt
     5461 tttgtggtcc atatcgacca ccgcgccatt ttcagagata aacttcagct cttccgcaga
     5521 catacgtggg tgatctgtcg ggttatgaat caacttgatc cacagcgccg tcagcacaaa
     5581 accaatcccc cccataacgg tgaagacgtg ctcccagccc caagcgaaag tcagccagcc
     5641 aagcagcggc gaaaagagcg ccagcgagaa atattgcgcc gagttaaaga tggcggaggc
     5701 agtaccacgt tctttcgtcg ggaaccaggc ggcgacaata cgggcgttcg ccgggaatga
     5761 tggcgcttcc gagaacccca gcataaagcg cataaagaac atggagatcc ctgcccaggc
     5821 cagcgggaac atatcaacaa agccttgcag gaaggtgaac agcgaccaga aaaagaggct
     5881 gtaggtgtaa acttttttcg agccaaactt atcaagcaac cagccgccgg ggatttgcat
     5941 cagcaagtag gcccagccaa aagcagagaa gatgtaaccc atcgaaaccg cacttaactg
     6001 caactctttt gccacttcgg taccagcaat agacagcgtt gcacgatcgg cgtagttaac
     6061 ggcggtaaca ataaaaataa tcagtaatat taaatagctg gtatgcacgc ctttcttttt
     6121 tacgtcaacg gtgtccagaa tcattttatt tacctcgggt acttatgctg atttttatta
     6181 ttatggggaa ggtgttattt atgagtttta tttatgccgt tacgacaatg aactcgggaa
     6241 ttagtataag cagcgggaga ataataatca ttgtgcaaat gctaatttaa tcagtactat
     6301 ttaaatatta ttttgagcat atgcacataa ggttgcgcgc taaagcacag atttgcgctt
     6361 taccttaccg ggcggcactg caatccctga aatgattgac attgatcaca tttctgcgtt
     6421 taaactcctg acattcttat ttcacccaat gaagtcattt atttttaaat gagaccagat
     6481 cctcatttta ataacccctg gctggagaat attgcacaat ggccaacatc gaaatcagac
     6541 aagaaacgcc aactgcgttt tatataaaag ttcacgacac agataatgtg gcaattattg
     6601 ttaatgataa tggcctgaaa gcaggaacgc gttttccgga tgggctggaa ttaattgaac
     6661 atattcccca ggggcataaa gtcgcattgc tggacattcc agctaatggt gaaattattc
     6721 gttatggcga agtgattggt tacgccgtgc gcgcaatccc acgcggaagc tggatcgacg
     6781 aatcaatggt tgtactaccg gaagcgccgc cgttacacac gctgccactg gcaaccaaag
     6841 tcccggcccc cttaccgccg ctggaaggat acacctttga gggctatcgc aatgccgatg
     6901 gcagcgtggg caccaaaaac ctgctcggca tcaccaccag cgtccactgt gtggcaggcg
     6961 tggtggacta tgtagtaaaa atcattgaac gcgatctgct accgaaatac ccgaacgtcg
     7021 atggcgtggt ggggctgaat catttgtacg gctgtggcgt ggcgattaac gcaccggcgg
     7081 cagttgtgcc tattcgtacc atccacaata tttcgctgaa ccctaacttt ggcggcgaag
     7141 taatggtgat tggtctgggt tgtgaaaagt tgcagcctga gcgcctgctg gtcggcacgg
     7201 atgatgtgca agctattcca gtagaaagcg ccagcattgt cagtttgcag gatgaaaagc
     7261 atgtcggctt tcagtccatg gtcgaggata ttttgcaggt cgccgaacgc catctacaaa
     7321 aactgaatca acggcagcga gaaacctgcc cggcttcaga actggtcgtt ggtgtgcagt
     7381 gcggtggcag cgatgcgttt tctggtgtaa cggcaaaccc ggcggttggc tatgcgtctg
     7441 atctactggt gcgctgcggc gcaacggtga tgttttcaga agtaacggaa gtgcgtgacg
     7501 cgatccatct gctgacacca cgcgcagtga acgaagaggt cggcaaacgg ctgctggagg
     7561 agatggagtg gtacgataac tatctcaata tgggaaaaac cgaccgcagc gccaaccctt
     7621 cgccgggcaa caagaaaggc ggtctggcaa atgtggtgga gaaggcactc ggctccattg
     7681 ctaaatcggg taaaagtgca attgttgaag tgctgtcgcc cggtcaacgc ccgactaaac
     7741 gcggattaat ttacgccgcg acgccagcca gcgattttgt ctgtggcacg caacaggtgg
     7801 cttcgggtat caccgtacaa gtgtttacga ccggccgtgg tacgccgtac ggcctgatgg
     7861 cggtacccgt aattaaaatg gcaacccgca ccgagctggc gaaccgctgg tttgatttaa
     7921 tggatattaa cgcgggcact atcgctaccg gcgaagagag cattgaagag gtgggctgga
     7981 agttgttcca ctttattctc gacgtcgcca gcgggaagaa gaaaaccttc tcggatcaat
     8041 gggggctgca taaccagctg gcggtgttta acccggcacc ggtgacctga tttcttttgg
     8101 aacgtccctc gcaaaacatg gccttagtgc catgttttta ttgtttaaag cccccacgtc
     8161 cattaataat gcatttgcat taccttagtt caagcttata atttgagcag aaaacaggct
     8221 gtaaaaggac aatgaatcat gcccgctaat gctcgctctc acgctgtact gaccactgaa
     8281 tcaaaggtca cgatacgcgg acaaacaact atccccgcgc cagtgcgtga ggccttaaaa
     8341 ctgaagccag gccaggacag cattcattac gaaattctgc ctggtgggca agtatttatg
     8401 tgccgactgg gagatgaaca ggaggatcat actatgaatg catttttgcg ttttctggat
     8461 acagatatcc agaacaaccc gcaaaaaact cgtccattca acattcaaca ttcaacattc
     8521 aacattcaac attcaacatt caacattcaa cattcaacat tcaacattca acattcaaca
     8581 aggaaagaaa cttgtcgctg gcatggacgt caacattgat gatgagattg gcgacgacga
     8641 ataatggatt ttccacaaag ggttaatggt tgggcgctat atgctcatcc ctgttttcag
     8701 gaaacctacg acgctttagt tgccgaagtc gagacattaa agggaaaaga tcctgaaaat
     8761 tatcagagaa aagccgccac aaagttattg gcggtagtcc ataaagtgat tgaggagcat
     8821 atcacggtca atccatcatc accggcattc cgtcatggca agtcgttagg ctctgggaaa
     8881 aataaagact ggtcacgggt aaaatttggt gcaggtcgtt atcgtctctt ctttcgttat
     8941 agtgaaaaag agaaagtcat cattctggga tggat
//
LOCUS       DAESHI010000155         8828 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.157, whole genome shotgun sequence.
ACCESSION   DAESHI010000155 DAESHI010000000
VERSION     DAESHI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8828
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.157"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(1..883)
                     /locus_tag="KW011_003863"
                     /pseudo
     CDS             complement(1..883)
                     /locus_tag="KW011_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    complement(487..603)
                     /locus_tag="KW011_003863"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            872..1693
                     /locus_tag="KW011_003864"
     CDS             872..1693
                     /locus_tag="KW011_003864"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBH7662090.1"
                     /translation="MFSHPVYLFLREFSLQDSRGGSLSKKAESLSSGKITIGTKEKVI
                     IINELRQCHPLSQLLVIADLPRSTFYYHVKRLNAPDPYQLVKQVILRIYHQHKGRYGY
                     RRIRLACRNEGILLNGKTIRKLMKELGISSLIRRKKYRAYRGEQGRTCNNLLKRQFYA
                     DRPNQKWVTDVTEFKVDGRKLYLSPIMDLYNGEIVSYNLTERPLASMVKSMLLDAVEQ
                     LNKDDKPLLHSDQGWQYQMPRWQRWLSDNGITQSMSRRGNCLDNAAMESFFSTLK"
     gene            1697..1840
                     /locus_tag="KW011_003865"
                     /pseudo
     CDS             1697..1840
                     /locus_tag="KW011_003865"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2230..2811)
                     /locus_tag="KW011_003866"
     CDS             complement(2230..2811)
                     /locus_tag="KW011_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662091.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGECFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(2858..6715)
                     /gene="sigA"
                     /locus_tag="KW011_003867"
     CDS             complement(2858..6715)
                     /gene="sigA"
                     /locus_tag="KW011_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HBH7662092.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFLSSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASSGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(7077..8081)
                     /locus_tag="KW011_003868"
                     /pseudo
     CDS             complement(7077..8081)
                     /locus_tag="KW011_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            8066..8443
                     /locus_tag="KW011_003869"
                     /pseudo
     CDS             8066..8443
                     /locus_tag="KW011_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8482..>8828)
                     /locus_tag="KW011_003870"
     CDS             complement(8482..>8828)
                     /locus_tag="KW011_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH7662093.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         2271 a         2001 c         1984 g         2572 t
ORIGIN      
        1 acggacgacg ggcccacaac gcctgctcca gtgcatccag cacgaatgtc gtttccatag
       61 acgatgagac tcgtcaccac acgatgtatc cggcaaacac atcaatgatg aacgccacat
      121 agacgaagcc ctgccatgtg ctgacgtaag taaaatcagc cacccacagc tggtcaggac
      181 gttctgccac gaactgacgg tttacgcggt cacctgcggc aacggttttc cggctgacgg
      241 tagtgcggac ctttttaccc cggagaacac cgacaagtcc cataaccgcc atgaggcgcg
      301 ccactgtaca tctggccacc ctgattcctt cccgtaacaa ctggcgccag actttacgca
      361 caccgtacac ctgatgattt tcatcgtata cgcgctgtat ctctctcttc agccagtcat
      421 cgtgctgcgc acgggcactg cgtttatcag gatgatgtcc tgttgctaac agtggtaata
      481 cgttgacggg gcaatatgca gttcactgca taccggtccg accccgtact gcccacgcag
      541 cttatccagc agtggcatta ttttttccag aggcggtcga actccgcctt cgcaaaataa
      601 gcggaagcct ggcgaaggat atcgttactg cagcgcagtt cacgattctc acgctccagc
      661 tctttcagac gctgacgttc agcggtggtg agccctccat caccgcccct ggtatcccgc
      721 tcatgctggc gaacccagac acgcagagtc tccggtgtac agccaatctt tggagtaatg
      781 gaacaaatta ccgcccattg tgagtcatat tcgccctgac tttccagaac catacggact
      841 gcccgttgac ggacttcggg ggaaaacgag tatttttagt catcctgttt acctctttct
      901 cagggagttt agtctccagg attcccgggg cggttcatta tctaaaaaag ctgaaagcct
      961 tagctcagga aaaattacga ttggaacaaa agaaaaagta atcattatta atgagttaag
     1021 gcaatgtcat ccattatcac aattactggt tatcgcggat ttgccacgta gtacatttta
     1081 ctatcacgtg aaacgcctta atgcgccaga tccatatcaa cttgttaaac aagtaatatt
     1141 gagaatatac catcagcata aagggcgtta tggttatcga cgcatcagac ttgcctgccg
     1201 taacgagggc attttactta atggtaaaac catcagaaag ttaatgaagg aactgggtat
     1261 cagctcactt atcaggcgca agaagtaccg tgcctatcgt ggagaacagg ggcgaacttg
     1321 taataattta ctgaagagac aattttatgc tgaccgccca aatcaaaagt gggtaacaga
     1381 tgtcacagag tttaaggtcg atggcaggaa gctctattta tcaccaatca tggacctgta
     1441 taatggagaa atagtctcat ataaccttac tgagcgccca ttagcatcaa tggtaaagag
     1501 catgctatta gatgcggtag aacaactcaa taaagacgat aagcccttgt tgcatagtga
     1561 tcaggggtgg caatatcaga tgcctcgttg gcaacgttgg ttgagtgata atggcataac
     1621 acaaagcatg tcccgacgtg gtaactgctt agataatgca gcaatggaaa gcttttttag
     1681 tactttaaaa taagaatgtt actacctaaa taaatacaat aagttacatc atctaaagca
     1741 agatataatt gattacattg aatactacaa tcaccatcgc attaaagaga aactcgatgg
     1801 catgagtccg gttcaatatc gacttaaaca tttttcatag gatgtccaag atttggggcg
     1861 cagatcattt gaaaaggtgg gcacctacgt taccaatact ggcttaatgg ctacatacgg
     1921 cagtcggttt acgcttacct ccgttaacgc ttacctccgt taacgcttac ctccgttaac
     1981 gcttacctcc gttaacgctt acctttaatc tgctgaattt cgaattgttc aggagtaata
     2041 gtccaaagta tcgggaattt tccagacaat cctgataata cgcttatgaa cattacaaca
     2101 cgctcatgcg gaacctggat gggggaagga acggtgaagc tgttagcgaa tgaccggcgt
     2161 ttgtataaac gccggtcatg atgaatgata tttcagaata tacctcgggc taaaataccg
     2221 ggacagagat caggcgtgat taatctgcag catttttaat aacagaagta ctgagcttac
     2281 tttacctttg atgatgtaat agttatttcc gtcggtactc atttttccct gaaataatgt
     2341 cacggatgtt tcttcctgct gatcctgcag gcgcagtcgt aaggtcatat ttttaatgag
     2401 ctgagatccg ggtggcaagg ggtatttgct gtcagatact gggcgactga gtctgatgtg
     2461 gcgcgtatac agcccgtttt cgtacagggt ggcctctgtt atggtgtcag tgaccagcat
     2521 gtgggagaca atgctgtatt cttttttaaa gcactcacca gaaacggtga ttgtaatgat
     2581 aatggtcttc tcgatcgaga tgtaaatacc cgtttcgtta tactgtccgt atcggaacca
     2641 tgagaactca gttgcattcc agccatgctc cagaatatgt gcattttttt gcctgatagt
     2701 ttcttttttt attcgctcat gtttaaggca taacaggcag ataaataatg tcagaaaaag
     2761 aaagctaata atgagagcaa gagcgatata gtctgtcaca gggtaataca tattctctac
     2821 ggtagaagaa gggccgcaaa cgcggcccgg gctgttatca gaaagagtaa cggaagttgg
     2881 cgttgatcgc gttatccacg ttgtatttac caaatgccga tttctcaaac tcaagaccga
     2941 agcgaagatt atcgcgaatt tcggcgttga gaccaacatt catgagaata cgaccgtctt
     3001 tttcaccttt gatacgtttc tcaccggacg catcacgcag tacggtttca ccgttggcaa
     3061 acaggtcaaa ctggtagcca aggccggcgc gggctgtgac tttccagtcc ttaccggaga
     3121 aggatttacc cacatcaaca ccggtacgcc caatcagcgg attaaagtcc ttatccttca
     3181 tggtcacgct cattccgcga tcctgccagt cgaaccgttt acctgatacg gccccataaa
     3241 ccagttctgc ctgcggctca atccaggagt cttctgttac atgatagcgg tagcctgctt
     3301 cagcacccac atacaaggaa tgagaactgt agtcttttgt tccgagtcca gcaaaggtcg
     3361 ccgagtactc attatcatgg tgaacatact tactaatcag gtcgatatag gcaccagagt
     3421 caaatatagc ggaagcatac agacctgccc cgacggactt cgtcttgcca ctgaatgcat
     3481 tactgctggc atgactgtcg gtatacgtca tagtcagacc agtgaaaagg tcaagtccat
     3541 ccagctcatg ttttttatcc acaccaatct gcacatgggt gtagttgtca ctgtatccac
     3601 tagaagctga acctgctccg ctcatgatgc gggcccatgc accggcctcg ccattggtgt
     3661 cacgcagatc ccccattcgt ttattcaggt tgttgacttc tgtcaggaag gctttgtaat
     3721 taccggacat atacgatttt gctgcaccgg aagatgctgt attttcagcc accttatatc
     3781 cggtcagggt ccacgtggta tgttcctgat cctctttcgt gctgatcaca ggagatatgt
     3841 tgctgaaacc tatggcctga gtactggctt taaatatctc tttattgctt ccttttgggg
     3901 ctgttatcag gtcaacgttt aatcctgagt tattatttcc cactttctgt atatcgaccg
     3961 acagactgtt attgcttccg gttaactgat ttttaacaat gagtttatcc gattgattta
     4021 cagactggct cagatcagcc cgcataatca cagcagtatt agtggcatcc agtgtgttta
     4081 ctgtcacagt atggaaaccc gaattctcta tgttactaaa caacaaaaga ctgtcgcggg
     4141 tttttaatga ttgcagggct gactgtccgt ttactctcca taaggtgtta tacatgctgg
     4201 ctgaggcagc aggagcatca atacctccgg tataagaagt atcaaagcca cccagcaaac
     4261 ctgtagccag cggtgtgtgt gtggttttgt ccaagttatc ttctttatac tggtcaacac
     4321 caaacagaat agatgctttt ttgtctgatt ttatattacc attgacccag gcataatttt
     4381 gagcctttaa cgtggcacca tcttctgtca tattatatcc atctgacata tagattgccg
     4441 ggctgtattt attattttct gtaggggtgc ctgttaatgt cagggttccg gccagttcaa
     4501 tagctttatc tacatacatt tttccctggg cggtaacagt agctcctttg ttgataatca
     4561 ggtctgtatt aattaaggat aatgcaccag tcatagtaac agttgattta tcaccaacag
     4621 ttattttgct gttttgagca ctcagactgc cgttgaaact actttcgcct gctgaatccc
     4681 cggagcgaag ctgttgtctg aatccaaaac cttcgccagt aatgtttctt cctgagaaca
     4741 tgtcaatgta tgcagtttta tttcccagaa caatttgaga gtttttagcc tctatgtgtc
     4801 cttttacatt agcatcccgg cctatactca gggttgcgtt ttctaaattt aatttccgga
     4861 aattaaattt acgactttcc cagtcgggct gggaaaaaga ggctatctga ttgttggact
     4921 tatattccgt attgtaacgt ttatttacag tgctttcctg gtcctttatg gcagcagaat
     4981 agtctttttg acataaaaaa ggaattggac agttgttatt gccttcttta aatatggcat
     5041 gttctgtcgc atgtccttgt aatgttaact gtgcattccg gacagagata tcgtttttga
     5101 tatcgactga gccatcaaag atcagattgg cattattgtt atgttgctgg gtagtattga
     5161 taatatgatt tatgcttata ttacctgata cattcccatg gtacatatat gcatcagtat
     5221 tagtaagtga taatgttgat tgcttcacgt ttgagttcgt tacggttgtt cccgcatctg
     5281 ttgctgcgat tttctgaaat gactggtcat agccgtttag atccagtgtt ccgccatttc
     5341 tggtaaaata gatttcgcca ttaccgcttt cgcttaaagc gtctttggca tttattttta
     5401 ccgtaccttt acctccggcc atgtaaattt tattgaaggc tttttcagca ctaaggatga
     5461 cagtcccatt acctatttta aggttatttc cctgtgctat ttttacatcc agagtaccac
     5521 tacctatttt atgcagatta tctccggcaa cgcccttaac attccattca acggtggtat
     5581 tattatcaat atctatcccg gcacctttat aagacttatc ttttccgtac actttatatg
     5641 tctggttctt atcgaatata agcccgccaa tgccaagatc ggtatcctgc ttgagcgtta
     5701 attcccctcc tcctgagaca gacaggtcct tatcttttat aatcgatgat aactcttgtt
     5761 ttttaccatc cagctgcata gtgtttgtga aattcttttt taaattgttt accagaatat
     5821 tattaaataa cgagtagttt gtccaggcta tattggtact actgagaaaa ccaactgtac
     5881 tggtaacacc atggaaaacc catttttgtt gttctttatc atatagataa gaaccagaac
     5941 cactatcgcc aaaaattggt gagtttttaa aaggggttct gttggtataa atgtaactct
     6001 tgtttttcca gtccagatta aaaagacttg cgcttaacaa gtcgggatta tagttttgtc
     6061 caaacagata ttgtttgccg tttaatatcg ttgaggttgt tccggcacct gctctgaagc
     6121 caattatttg ttccttaccg ttgtagttta cgccatatct tttcatcgca tcttcgggag
     6181 aaagtgagaa gtcggcactc tccgtaaccc ctgttgtttc cacgacgaat ttattcagac
     6241 gatgaaccga aaagtcgcca gatgatactc tgttacttgc tttatatgct gtctgatccc
     6301 atgactgtgt tgttattgca tgatgctgtg taccgttatg agtcgcagta acactgtacg
     6361 cacctccaat tgatgttgtt gcacctttgt tggaaacagc agaaaaatca ggtacaggta
     6421 gttctggaaa atgaaatatc tttccatctt tcatcattaa ttgaacattt gttgcacccg
     6481 gtctgaactc tcctctgttt tgagcaaggt caagataatc gcgggtccat atattattta
     6541 tatccagcat tgctgcaaat gctgtttcgc cataacttcc atatattgag gataaaataa
     6601 taccgaggat aactttcttt cttgatgtac cgacactaac tttccgggtc agttcagaaa
     6661 cagcaactaa tccacctgta atatgactat atttcagtga ataaatttta ttcatacgtg
     6721 aactccgata tttctgagtt gtggttatgg gatagcattt aattacaacg gggaactatc
     6781 agtcatggta cgtaactgat aagggtataa tgataatcac tgtcagttat atttgtatga
     6841 cagatgtaac aaaatgaaaa aaaaagataa gcacaagtga ttgattggtc agatattgat
     6901 cacttggtgt ttgtattttg aactttgcga tcaaaatttg atctttgttg tttccggtca
     6961 atcctttaca ggttttctgt attatccggg ggagttaagt ctgtcgatgt ccgacagact
     7021 tttgatgatg gaaacaacat gcagcgataa tgtgcatgct gttttgcagg tggaactcag
     7081 aaggtgaact ggaactgtgc gaccgcaccg taagtgttgt ccgtgccgat caggcctgca
     7141 acatcaaggt gaaccacatc aaacggtgag atccccaccc cggccgtgaa tgcactgccg
     7201 tcgttaccgg ccagattctg acggtaaccg gcgcgcagct gtgcccattt ccaggcatta
     7261 aattctgcgc caatggcggc aaactgacgg ttgctgtcgg aggtgaaacc gctggccggg
     7321 gtcagatcca catcaaatgc ggtggtgaac atcgcgttgt gccaggagac accggccgtc
     7381 acctgcgggc ggatcctgaa ggtttccgtg ataccgttca cttcttttgt ctcaatacta
     7441 cgggggatca ggttctgtgc gaccagtccc agcgtccagt tgtcatccag gtccgtactg
     7501 gcaccgatgt cggcgttgat gccatttttc gtgttgtggt agcggtcgcc tttgaaggcg
     7561 ctgctgtcat aatttctgac cagcacgttg tagttgaaca ggtcgacgcg ctggaatttg
     7621 ggggggaagc ccagagacca tttctgcccg gccgtttcca gttccgtagc cagagcaata
     7681 cccacatcag tgatcagtgc cgcgcgggca aaggcctgtg atgtcaggcg gcttttgtcc
     7741 acctcctgcc cggtgctgtt tgccacctct tccaggtaag tgaggtcagc atcagagact
     7801 ttaccgtcga cactgacggt gccgtgtgat ttaaccatca gtgcggcaga gaggcgatca
     7861 ccgggaaggg ccgccacagc agaaacgccc agctgtgcgg cggcatgtgt gttacggaag
     7921 tcccggaggt gttctttcag gcgtgcggca gcctcaggaa caccgtggtt actttcgatg
     7981 gcgcggtcga aggccttcca ctcatcactg atccggtcgg cgttgtcggt gatattatcc
     8041 ggattggaga gctgcgcgcc gacggacgga agcaggatgc tgggctcatg cgcgacgcaa
     8101 aatccacgat gtatatatca gtactaaaag cgcgacggcg gaagaagctc tgaaactaat
     8161 cggtgaactg tacgccatag agcacgatat acgcgggtta ccggtgtctg aacgcctggc
     8221 ggtcaggcaa atgcagagta gaccgctcct gacttccctg tataagctga tgcaggagaa
     8281 agaacacacg ttatcgaaga aatgtcgtct gggagatgcg ttccggtata tcaggaagca
     8341 ctgggctgcg ttgtgcaact tctgtgatga tggcctggct gaggtagata ataatgcagc
     8401 agaaagagcg cttcgtgcag tctgtctcgg gaagaaaact ttttcgagga tcactcattc
     8461 cgtgtctgac tgcttcaggg tttaattttg cttctgctga ctgaatgatg attgggcagc
     8521 aagtgcaata tcccacataa atccagttaa ctcacgagcc accgccgtaa tggctacttg
     8581 tgattttttc ccttttgctg tcagagttcg gtaacgagaa cacaatcgtt gttgagcttt
     8641 ccatgcaata tctttggaat attgtgttac agagtccggt cggtaatata taagccatgc
     8701 tccaactttt gcaggtgtac gataagacca agcggcttcg taaagcagac gtctcagttc
     8761 gctgtttcca acttttgtta ttcctctggg acgaatactg tttccactgg aatattcacc
     8821 tggcacca
//
LOCUS       DAESHI010000156         8773 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.158, whole genome shotgun sequence.
ACCESSION   DAESHI010000156 DAESHI010000000
VERSION     DAESHI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8773
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.158"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(406..612)
                     /locus_tag="KW011_003871"
     CDS             complement(406..612)
                     /locus_tag="KW011_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HBH7662094.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            925..1014
                     /gene="kdpF"
                     /locus_tag="KW011_003872"
     CDS             925..1014
                     /gene="kdpF"
                     /locus_tag="KW011_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBH7662095.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1014..2687
                     /gene="kdpA"
                     /locus_tag="KW011_003873"
     CDS             1014..2687
                     /gene="kdpA"
                     /locus_tag="KW011_003873"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBH7662096.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLIALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2710..4758
                     /gene="kdpB"
                     /locus_tag="KW011_003874"
     CDS             2710..4758
                     /gene="kdpB"
                     /locus_tag="KW011_003874"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBH7662097.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4767..5339
                     /gene="kdpC"
                     /locus_tag="KW011_003875"
     CDS             4767..5339
                     /gene="kdpC"
                     /locus_tag="KW011_003875"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBH7662098.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5332..8016
                     /gene="kdpD"
                     /locus_tag="KW011_003876"
     CDS             5332..8016
                     /gene="kdpD"
                     /locus_tag="KW011_003876"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HBH7662099.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            8013..8690
                     /gene="kdpE"
                     /locus_tag="KW011_003877"
     CDS             8013..8690
                     /gene="kdpE"
                     /locus_tag="KW011_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HBH7662100.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
BASE COUNT         1840 a         2286 c         2529 g         2118 t
ORIGIN      
        1 gcaccgagca ggggattgac cggatggccg gacgtcactc cgccggggca caccgaacag
       61 gccacaccgg tgggggcacc gatgcgcacc ccggctgaac cctggacaat gctaccgcca
      121 tactgcgtca tgtcgccctg acgcgccgcc ggttttccgc tcatttctca tccttgtttt
      181 atcgggttat ttgtccgctg acaggcaaga ccctacagat ttataagaaa gccttaagtg
      241 agatatccgt cagatagaaa caattaggaa aatatttata tatatttcat gaatatatgt
      301 ttcgattggt ataacggtgt ctaaatattc ctctgaacat cttcctgata caaaaaaccg
      361 ccagcgtttc cgctggcggt tttcaatgta ttcagaactc agcaatcagt aaaaatcacc
      421 ggttgccttt tcggcctgat ccatccacac cggtttatcg ctggttttcg accagacgcg
      481 gtgcaggtag ctgtaaaagc gggcgcggtc tttccagaaa agcatgaaag gcagcgccag
      541 aacgcccgct gcaaccgcat atgtacggcg taaaaagata agccatgcag gatattctct
      601 gtagagttcc atacttttct cccccttttg tatctacccg gtgaatagca ccggaaaaat
      661 gaatttgttt atctgatgaa aatagtaccg ccttttgtgt aattttacta ctcatccgac
      721 cacttatttt tgcttattga tggtttattt acattcactc tgtaattaag ttacacaaaa
      781 gttaaattaa tactaaacat tagttaaatc atggcttttg ccatttttat acttttttta
      841 caccccgccc gcagattttt gcgaaatctt tgcagccaga attctactct tccggtatca
      901 cttttaggcc actggaggtg cactgtgagt gcaggcgtga tagccggcgt gttgctggtg
      961 tttttattac tgggttattt ggtttatgcc ctgatcaatg cggaggcgtt ctgatggctg
     1021 cgcaagggtt cttactgatt gccacgtttt tactggtatt aatggtgctg gcgcgtcctt
     1081 taggcagcgg gctggcgcgg ctgattaatg acattcctct tccaggtaca gcgggcgttg
     1141 aacgtatact ctttcgttta cctggcgtct ctgaccatga gatgaactgg aagcaatatc
     1201 tttgtgccat tctcggcctg aacatgctgg ggctggcggt gctgtttttt atgttgctcg
     1261 gtcagcacta tctgccgctt aatccacagc agttgccggg gctgtcgtgg gatctggcgc
     1321 tgaataccgc cgtcagcttt gtcacgaata ccaactggca atcttatagc ggtgaaacca
     1381 cgttgagcta tttcagccag atggcaggct taacggtaca aaactttctt tctgccgcca
     1441 gcgggattgc ggtgattttt gccttcatcc gtgcgtttac tcgccagagc atgagcacac
     1501 tcgggaatgc ctgggtcgat ctgctacgca tcacgttatg ggtgctagtc cctgtggcgt
     1561 tgttgatcgc actgtttttt attcaacaag gtgcgctgca aaactttctg ccctatcagg
     1621 cagtgaatac cgttgaagga gcgcaacagc tgttacccat gggaccagta gcttctcagg
     1681 aagcgatcaa gatgctcggt actaacggcg gtggcttctt taatgccaac tcgtcgcatc
     1741 cgtttgaaaa cccaaccgca ctgaccaact tcgtgcagat gctggcgatc ttcttgatcc
     1801 caacagcgct gtgctttgcc tttggtgaag tgacgggcga tcgccgccag gggcgcatgt
     1861 tgctgtgggc gatgtcagtg atttttgtca tctgcgtagg cgtggttatg tgggcagaag
     1921 ttcagggtaa tcctcatctg ctggcgctcg gcgcagacag cagcatcaat atggaaggta
     1981 aagagagccg tttcggcgtg ctggtcagta gcctgtttgc ggtcgtgact acggcggcgt
     2041 cctgtggcgc ggtgattgcg atgcatgatt cgtttaccgc tctcggcggc atggtgccga
     2101 tgtggctaat gcaaattggt gaagtggtgt tcggcggtgt cggttctggt ctttacggca
     2161 tgatgctgtt tgtcctgctg gcggtgttta ttgccgggct gatgattggt cgtacaccgg
     2221 aatatctggg taaaaaaatc gacgtacgcg agatgaaact gattgcactg gcgattctgg
     2281 ttactccaac actggtactg atgggcgcgg cgttggcgat gatgaccgac gccggacgta
     2341 gcgccatgct taaccctggc ccgcatggtt ttagcgaagt gctgtacgcc gtgtcgtccg
     2401 ccgctaacaa caacggcagc gcctttgccg gattaagcgc caactctccg ttctggaact
     2461 gtttactggc gttctgcatg tttgtcggtc gcttcggggt gattatcccg gtgatggcaa
     2521 ttgccggttc gctggtgagt aaaaagagcc aacccgccag ctccggcacg ctgccaacgc
     2581 acggcccgct gtttgttggc ctgttaatcg gcaccgtgtt gctggttggc gcactgacct
     2641 ttatccctgc cctggcgctt ggtccggtgg cggaatatct ctcctgatga tattgagcga
     2701 gcactgaata tgagtcgtaa acaactggcg ctattcgaac caacacttgt cgttcaggcg
     2761 ctgaaagaag cggtgaaaaa attaaacccg caggcgcaat ggcgcaatcc ggtgatgttt
     2821 atcgtctgga tcggcagtct gctgaccacc tgtattagca tcgcgatggc aagcggtgcg
     2881 atgcctggca atgcgctgtt tagcgcggca attagcggct ggctgtgggt caccgtactg
     2941 ttcgctaatt tcgccgaggc gctggcagaa ggccgcagta aagcgcaggc caacagtctt
     3001 aaaggggtga aaaaaactgc ctttgcccgc aagctgcgtg agccgaaata tggcgctgcg
     3061 gcggacaaag ttcctgccga tcaactgcgt aaaggtgaca tcgtgctggt agaagctggc
     3121 gatattatcc cctgcgatgg tgaagtcatt gaaggcggcg catcggtcga tgaaagcgcc
     3181 atcaccgggg aatcggcacc ggtgatccgt gaatccggcg gcgattttgc ctccgtcacc
     3241 ggcggcacgc gtattctttc tgactggctg gtgattgagt gtagcgttaa ccccggtgag
     3301 acatttctgg atcggatgat cgcgatggtg gaaggcgcac agcgacgcaa aacaccgaac
     3361 gagattgccc tgactatcct gctgattgct ctgaccatcg tctttttact ggcaaccgcc
     3421 acgctgtggc cgttttccgc gtggggcggt aatgccgtga gtgtaacggt actggtggcg
     3481 ctgctggtct gtctgatccc aaccactatt ggcggcctgt tgtcagcgat cggcgtcgcc
     3541 gggatgagtc ggatgctggg cgcgaatgtg attgccacca gcggacgtgc agttgaagcg
     3601 gcaggtgacg ttgacgttct gctactggat aaaaccggca ccatcacact cggtaaccgt
     3661 caggcgtcgg agtttatccc cgcgcagggc gtggatgaaa aaacgctggc tgacgccgca
     3721 caactggctt cgctggctga tgaaacgccg gaaggtcgca gtattgtgat cctcgccaag
     3781 caacgtttta acctgcgcga gcgcgatgtg cagtcgctcc atgccacctt tgtaccgttt
     3841 actgcgcaaa gccggatgag cgggatcaac atcgacaacc gcatgatccg taaaggttct
     3901 gtcgatgcca ttcgccgcca tattgaggct aacggcggtc acttccctgc cgatgttgat
     3961 caaaaagtcg atcaggttgc gcgtcaggga gccacgccgc tggtggtggt ggaaggttct
     4021 cgtgtgctgg gcgttattgc gctgaaagat atcgtcaaag gcggtattaa agagcgcttc
     4081 gcccagctgc gcaaaatggg cattaaaacg gtgatgatta ccggcgataa ccgtctgact
     4141 gccgccgcga tagctgcgga agcgggtgtc gatgattttc tcgccgaagc aacaccggag
     4201 gccaagctgg cattgatccg tcagtatcag gcggaaggtc gtttggtagc gatgaccggc
     4261 gacggcacca acgatgctcc ggcgctggcg caggcagatg tcgcggtggc gatgaactcc
     4321 ggcacccagg cggcgaaaga ggcgggcaat atggtggatc tcgactctaa cccgaccaag
     4381 ttgatcgagg tggtgcacat tggcaaacag atgctgatga cccgtggctc gctgaccacc
     4441 ttcagcattg ccaacgatgt ggcgaaatac ttcgccatta ttccggcggc gttcgcggca
     4501 acatatccgc agttaaatgc gctgaacatc atgcgcctgc attcgcccga ctccgccatt
     4561 ctcagtgcgg tcattttcaa cgccttgatt atcgtctttt tgattcctct ggcgttaaaa
     4621 ggcgtgagtt ataaaccgct taccgcttct gccatgttgc gccgtaactt atggatttac
     4681 ggtctgggtg ggctgctggt gccgtttatt ggtatcaaag tcattgattt actgctgacc
     4741 gtttgcggtc tggtgtgagg tttaccatga gtggattacg tccggcatta tcgacatttc
     4801 tctttctgtt attgattact ggcggcgttt acccgctgct gaccaccgca ctggggcaat
     4861 ggtggtttcc ctggcaggcc aacggttcgt tgatccgtga aggtgatacg gtgcgtggct
     4921 cggcattaat cgggcagaat tttaccggca acggctattt tcatggtcgc ccgtcagcaa
     4981 cggctgaaat gccctataat ccacaggctt ctggcgggag caatctggcg gtcagtaacc
     5041 ctgagctgga taaactaata gccgcacgcg ttgctgcatt acgggccgct aacccggatg
     5101 ccagcgcgag cattccggtt gaactggtga cggcatcggc aagcgggctg gacaataata
     5161 tcaccccgca agcggcggcc tggcagatcc cacgcatcgc gaaagcgcgt aatctcagcg
     5221 ttgaacagct cacgcagctg atcgcaaaat acagccaaca accgctggtg aaatatatcg
     5281 gccagccggt cgtcaacatt gtcgaactca atctggcgct ggataagctt gatgaataac
     5341 gaacccttac gtcccgaccc cgatcgtctg ctggaacaaa ctgccgcgcc gcatcggggg
     5401 aagctgaaag ttttcttcgg tgcctgtgca ggcgtcggga agacctgggc gatgctggca
     5461 gaagcccagc gactgcgggc gcaagggctg gatattgtgg ttggcgtggt agaaacccac
     5521 gggcgaaaag ataccgccgc aatgctggaa gggctggctg ttctgccgcc aaaacgcctg
     5581 gcgtaccgtg ggcggcacat ctgcgagttt gacctcgatg ccgccctcgc ccgccgtccg
     5641 gcgctgatct taatggacga actggcgcac agtaatgcgc caggttctcg tcaccctaaa
     5701 cgctggcagg acatcgaaga gctgctggaa gcaggcattg atgttttcac taccgtcaac
     5761 gttcagcatc tggaaagtct gaatgatgtg gtcagcggcg tcaccggaat tcaggtacgg
     5821 gaaaccgtgc ccgatccttt tttcgatgcc gccgacgacg tggttctggt ggacttgccc
     5881 ccggacgatc tgcgccagcg gctgaaagaa ggcaaagtct atattgccgg gcaggcggag
     5941 cgcgccattg aacatttttt ccgcaaaggc aatctgatcg ccctgcgcga actggcactg
     6001 cgccgtactg ccgatcgcgt tgatgagcaa atgcgcgcct ggcgggggca tcctagcgaa
     6061 gagaaagtct ggcacacgcg cgacgcgatc cttttatgca tcggccataa caccggtagt
     6121 gagaagctgg tccgcgcggc ggcgcggctg gcgtcacggc tgggtagcgt ctggcacgcg
     6181 gtgtatgttg aaacccctgc cctgcatcgc ttaccggaga aaaaacgtcg tgcaattctc
     6241 agcgccttac gtctggcgca ggaactgggc gcggagacgg caacactttc tgatccagcg
     6301 gaagagaaag cggtagtgcg ttatgcccgt gaacataatc tcggcaagat tattctcggt
     6361 cgcccggcct cgcgccgctg gtggcgtcgg gaaacgtttg ctgaccgact ggcgcgcatc
     6421 gcccccgatc tcgatcaggt gctggtcgcg cttgatgaac cacccgcccg cacgattaac
     6481 aacgcgccgg atagccgctc ttttaaagac aagtggcggg tacagattca gggatgcgtg
     6541 gttgccgccg cgttatgcgc cgttatcacc ttaattgcca tgcagtggct gatggcgttt
     6601 gatgccgcca acctggtgat gctgtatctg cttggcgtgg tggtggtggc gctattttat
     6661 ggacgctggc cttcggtggt tgccaccgtc attaatgtgg tgagtttcga tctctttttt
     6721 atcgccccac gcggcacgct cgccgtctct gatgtgcaat atctgctgac cttcgcggtg
     6781 atgttaaccg tcgggctggt gatcgggaac cttactgctg gcgtgcgtta tcaggcgcgg
     6841 gtagcccgtt accgcgagca acgcacacgg cacttatatg aaatgtcgaa agctctggcg
     6901 gtgggccgca gtccgcagga catcgccgcc accagcgaac aatttattgc ctccacgttt
     6961 catgcccgca gtcaggtgtt gttgcccgat gacaacggta aattgcagcc gttaacacat
     7021 ccgcaaggaa tgacgccatg ggacgatgcc atcgcgcagt ggagttttga taaaggcctg
     7081 cctgcgggcg cgggcaccga cacgttaccc ggtgtaccgt accagatttt gccgctaaaa
     7141 agcggcgaga aaacctacgg gctggtggtg gtggagccgg ggaatctgcg ccagttgatg
     7201 atcccggaac agcagcgcct gctggagacg tttacgctgt tagtcgccaa tgcccttgag
     7261 cggctgacgc taaccgccag cgaagaacag gcgcggatgg caagcgaacg tgaacagatc
     7321 cgcaacgccc tgctggcagc gctttcgcat gatttacgca cgccgcttac ggtgctgttt
     7381 ggtcaggcag aaatcttaac gctcgatctg gcaagcgaag gatcacccca cgcccgccag
     7441 gccagcgaga ttcgtcagca tgtgctgaac actacccgac tggtgaataa tctactggat
     7501 atggcgcgaa ttcagtccgg cggctttaat ttgaagaaag agtggttaac gctggaagaa
     7561 gtagtcggca gcgcgctgca aatgctggaa ccgggtttat cgtcgcccat caatctttct
     7621 ctgccagaac cgctgacctt aatccacgtt gacgggccac tctttgaacg ggtgctgatt
     7681 aatctgctgg agaacgcggt gaaatatgcg ggtgcgcagg ccgaaattgg tatcgatgcc
     7741 cactttgagg gcgaaaatct acaactggat gtctgggata acggccccgg tcttccgcca
     7801 ggccaggagc agacgatatt tgataagttt gctcgcggga ataaagagtc ggcggtgccg
     7861 ggtgtcgggc ttgggttggc aatttgtcgg gcgattgtgg atgtacacgg cggcaccatt
     7921 accgcgttca accgaccgga aggtggtgcc tgttttcgtg ttacacttcc ccagcaaacc
     7981 gctcctgaac ttgaagactt tcatgaggat atgtgacaaa cgttctgatt gttgaagatg
     8041 aacaggctat tcgtcgcttt ctgcgcacgg cgctggaggg cgacgggatg cgcgtctttg
     8101 aggccgaaac tctgcaacgc ggcttgctgg aagcggcaac ccgtaagcca gatttgatta
     8161 ttctcgatct cggcctgccc gatggtgatg ggattgagtt tatccgcgac ctgcgccagt
     8221 ggagcccggt gccggtgatt gtgctttccg cacgcagcga agagagcgac aagatcgccg
     8281 cgctggatgc cggagcggat gattatctga gtaagccgtt tggcattggc gaattgcagg
     8341 cccgtctgcg cgtcgcttta cgccgccact ccgccaccgc cgcgcccgat ccgctggtga
     8401 aattttccga tgttaccgtc gatttagccg cccgcgtgat tcaccggggt gaggaagagg
     8461 tgcatctcac accaattgag ttccgcctgc tggcggtgct gctcaacaat gccggaaaag
     8521 tactcaccca gcgccagctc cttaaccagg tgtgggggcc aaacgcggtc gaacacagtc
     8581 actatttgcg tatttatatg ggacatctgc gacaaaaact ggaacaggat ccggcccggc
     8641 ctcgccactt cattactgaa accggtattg gatatcggtt tatgctttga attgttattc
     8701 aataaattcg cttaaatagt gattaattta ttattaaacc tgcgccgaat attattattt
     8761 ggcaggttta att
//
LOCUS       DAESHI010000157         8725 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.159, whole genome shotgun sequence.
ACCESSION   DAESHI010000157 DAESHI010000000
VERSION     DAESHI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8725
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.159"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..1781)
                     /gene="atoS"
                     /locus_tag="KW011_003878"
     CDS             complement(<1..1781)
                     /gene="atoS"
                     /locus_tag="KW011_003878"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HBH7662101.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGAT"
     gene            1948..4797
                     /gene="rcsC"
                     /locus_tag="KW011_003879"
     CDS             1948..4797
                     /gene="rcsC"
                     /locus_tag="KW011_003879"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HBH7662102.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(4883..5533)
                     /gene="rcsB"
                     /locus_tag="KW011_003880"
     CDS             complement(4883..5533)
                     /gene="rcsB"
                     /locus_tag="KW011_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HBH7662103.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(5550..8222)
                     /gene="rcsD"
                     /locus_tag="KW011_003881"
     CDS             complement(5550..8222)
                     /gene="rcsD"
                     /locus_tag="KW011_003881"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HBH7662104.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         2136 a         2026 c         2231 g         2332 t
ORIGIN      
        1 aaggttgcgc cgtagcccgg caaactggcg acgcgaatat caccctgatg ggcattaatg
       61 atgcgttgac ttaacgccag accaagcccg gttcctgagg ctttggtggt gaaaaaggga
      121 tcgaagatct ttttttgcag cgagagatca atgccacagc cgttgtcctc tatcgaaatg
      181 gcctgttgtg agtcgctgta ttgccaggtt tgaatgcgaa ttttccctcg tgcgctgata
      241 gcctggacgg cattgatcag gatattcagt agtacctgtt tgagcagttc acgatcggcg
      301 ttaatcgggc ttaattcatt atccagttcg cttatgaagt cgacccgcgc ttgtacgccg
      361 gcggtttgta ccagtaccag agtttcttca accaatgcat tgaggctgac ttgttgccat
      421 tgactgtggc gtggacgtga aaattcgagc aattgctgaa taactttgtt aattgaatcg
      481 atttctttga gtactacgga cagatattcc tgatgtattg ggtcactggt ttgttggcgc
      541 aagatctgta cataaccacg aatagccgtt aacggattac gtacttcatg cgcgacgcca
      601 gccatcagct cacccagtgt ggcgaggcgt tctgcttgcg ccatgcggcg ctgggtttct
      661 ttgcgggcag ttaaatcaga gaaaatcacc aaagcaccta tcatttcacc gtgcgtgtta
      721 tgaatacgac tggtagtgac actgagttca atggtgcggt cacgacctgg aaaactgatc
      781 tccagcgcca catgttcggt gccatgttcc agcgtatcca gtactggact gtagaactga
      841 gtattgtcga acaacatgga gtaaggctgc cctaccagtt catggcgttg atagccagtg
      901 ataacttctg ctgctgggtt catggtggtt acatcaccct ggcggtcaat ggcaatgacg
      961 ccatcggcag cgttttcaat aatcagatcg ttaagtgtcc gcgtttcacg cagtgcctgg
     1021 gcgaggttat taacactctg actgatttgc cccatttcac cgggcaattg tggtaatcga
     1081 gtgggaatat tttgtgccag agtcgagagg ccatcggtga tgatatcaat attggcgctc
     1141 aggcgacggg agaaaaggac aatcaatagc aggcttatca gcaaatcggc ggtgagcaca
     1201 atgataatcc tcacatccat tttccaggcc tggcggcgaa tatcttcggt taattcattg
     1261 gcccagatat agccgaggat ttcaccatta cgctcaatgg ggagcattga attcaaaata
     1321 tcgccgcgca cctgcctgcc tgaataaacc aaaggggtat ttgtacgcat gacttcgcga
     1381 ccagggtgat ctgcggcaat ggtaacgccg acattattct gatatagcgc tgaaggcgcg
     1441 taggtgatta tcgcatccag cattttgttg taataaccag cacctatgcc agggaaggcg
     1501 tgagtgatat tttcggtaat gggggcaagt tctgcattta atgcgcggat acgctcctca
     1561 cgtggtaagt cgatgtagag atcatagcga tcgcctagtg cctgattaag caggttgacc
     1621 acggcagaaa gttttttctc tttttcagat aagactgctg aacgtccttc cgtttctacg
     1681 atataaccaa tagtaagcgt tgggacaatg accatcagga ttgccatcag gatcatttga
     1741 ttgcgtaagc ggcgtggata aatccacttc atataatgca tgctgaaatc cactagtctt
     1801 gtccggtata tgacgattat cagaggttaa ggtgatgatt tctcggcggt gtatcatatt
     1861 ccagagaaga gagaacattg cggtaacacg cttttaccgc taccttaacc acactccatc
     1921 ggtcacctga ggcggagctt cgcccctttg aaataccttg cttcttttcg tacaaccctg
     1981 aaagcctcgc gctacatgtt cagagcattg gcgttagtgc tctggctgtt gattgctttt
     2041 tcatccgttt tttacatcgt taatgcgtta catcagcgag aatcggaaat tcgtcaggaa
     2101 tttaatctga gttccgatca ggctcagcgc tttattcaac gcacctctga tgtgatgaaa
     2161 gagctgaagt acatcgccga aaatcgctta tcggcagaaa acggtgtgct ttccccgcgt
     2221 ggacgagaaa cgcaggcgga tgtgcctgcg tttgaaccgc tgtttgccga ctccgattgt
     2281 tccgcaatga gtaacacctg gcgaggttct ctggagtcat tggcgtggtt tatgcgctac
     2341 tggcgcgata atttttctgc ggcttacgat ctaaaccggg tatttttaat cggcagcgat
     2401 aacctctgca tggccaattt cggtctgcgt gatatgccag tggaacgcga taccgcgttg
     2461 aaagctttgc atgaacgcat caataaatat cgaaatgcac cacaagatga tagcggcagt
     2521 aacctctact ggatcagcga aggtccgcgc cctggcgtcg ggtattttta cgcgttgacg
     2581 ccagtttatc tggcgaaccg gttgcaggcg cttttgggtg tcgagcagac catccggatg
     2641 gagaactttt tcttaccggg tacgttgccg atgggggtta ccattcttga tgaaaatggt
     2701 cataccctga tttcgcttac cggaccagaa agtaaaatta agggcgatcc tcgctggatg
     2761 caggaacgct cctggtttgg ctatacggaa gggttccggg agctggtgct gaagaaaaat
     2821 ctgccaccct catcgctaag catcgtgtat tcggtgccgg ttgataaggt gctggaacgc
     2881 attcgcatgt tgatccttaa cgcaattttg ctgaatgtgc ttgccggagc tgcattgttt
     2941 actctcgcac ggatgtacga gcgacgtatt ttcattccgg cggaaagcga cgccctgcga
     3001 ctggaagaac atgagcagtt caatcgcaag attgtcgcct ccgcgccagt gggtatctgc
     3061 attttgcgta ccgctgatgg cgtcaatatt ttaagtaacg aactggcgca tacctatctc
     3121 aatatgctta cgcatgagga ccgccaacga ctgacgcaaa ttatctgtgg gcagcaggtc
     3181 aattttgttg atgtcctgac cagcaacaat accaatctgc aaatcagctt cgtccattcg
     3241 cgctatcgta atgaaaacgt ggccatttgt gtgctggtgg atgtttcttc gcgcgtgaag
     3301 atggaagagt cgttgcagga gatggcacaa gcagcggaac aggcgagcca gtcaaaatcg
     3361 atgttccttg ccaccgtcag tcatgagctg cgaacgccgc tgtatggcat tatcggtaac
     3421 ctggatctgt tgcaaaccaa agagttaccg aaaggcgtcg atcggctggt gacggcaatg
     3481 aacaactctt ccagcctgtt gttgaaaatt atcagcgata ttctcgattt ctcgaagatt
     3541 gaatcggaac agttgaagat cgaaccgcgt gagttttcac cgcgtgaagt gatgaaccac
     3601 atcaccgcca actatttacc gctggtggta cgcaagcagt taggcttgta ctgctttatt
     3661 gaaccggatg tgccagtggc cttaaatggc gacccgatgc gtttacagca ggtcatctcc
     3721 aacctgttga gtaacgccat aaaattcacc gataccggct gtatagtttt gcatgtccgc
     3781 gctgatggcg attatctctc tatccgtgtt cgcgataccg gcgtggggat tccggcgaaa
     3841 gaagtggtgc gcttgtttga tcccttcttc caggtcggaa cgggcgtaca gcgaaacttc
     3901 caggggaccg gtctgggtct ggcgatttgt gaaaaactga tcagcatgat ggacggcgat
     3961 atctcggtag attcagaacc gggaatgggc agccagttta ccgtgcgtat tccgttgtac
     4021 ggcgctcagt acccgcagaa aaaaggcgtg gaagggttga gtggtaaacg ctgctggctg
     4081 gcggtccgca atgcgtcgct ctgtcagttc ctggaaacca gtttgcagcg cagcggcatc
     4141 gtcgttacaa catacgaagg gcaggaaccg actcccgaag atgtgttaat tactgacgag
     4201 gtagtgagta aaaaatggca gggtagagcg gtagtgacct tctgtcgtcg ccatattggt
     4261 attccgctgg agaaagcgcc aggggagtgg gtacacagtg tggctgcccc gcatgagcta
     4321 ccggcattgt tggcgcgtat ttatttgatc gagatggaga gcgacgatcc tgctaacgct
     4381 ctgccgtcga cggacaaagc ggtcagcgat aatgacgata tgatgattct ggtcgtggat
     4441 gatcatccga ttaaccgacg tctgctggca gatcagttgg gatcgttggg ctatcaatgt
     4501 aaaaccgcga atgatggtgt cgatgcgctt aatgtactta gcaagaatca tattgatatc
     4561 gtgcttagcg acgtcaacat gccaaatatg gatggttacc gcttgacgca acgcattcgt
     4621 cagttgggac tgacgttgcc ggtaatcgga gtaactgcta atgcgttggc tgaagagaag
     4681 cagcggtgtc tggagtccgg tatggacagc tgcctgtcga agccggtaac gctggatgtg
     4741 ataaaacaga cgctgacggt atatgccgag agggtcagga aatcgcggga atcgtaggac
     4801 ggataaggtg tttatgccgc atctggcatt cagtgcaaat gccagatgcg acgctgacgc
     4861 gtcttatctg gcctacaggt gattagtctt tatctgccgg acttaacgtc actgaagaga
     4921 gataattcag cagggcgata tcgttctcga cacccagctt catcatcgca gatttcttct
     4981 ggctactgat ggttttaata ctgcggttca gctttttagc gatctcggtc accaggaagc
     5041 cttccgcaaa caggcgcaga acttcactct cttttggcga gagacgcttg tcaccgtaac
     5101 caccagcact gattttttcc aacaggcgag aaacgctttc cggggtaaat ttcttccctt
     5161 tctgcagcgc ggcgagagct ttcggcagat cggtcggtgc accttgtttc agcacgatcc
     5221 cttcgatatc cagatccaat accgcactaa gaatcgccgg gttgttgttc atagtcagaa
     5281 caatgatcga caggcttggg aaatggcgct tgatgtactt gattaaggta atgccatcgc
     5341 cgtacttatc gccaggcatg gagagatcgg taatcaacac atgcgcatcc agtttcggca
     5401 ggttgttgat cagtgctgta gagtcttcaa attcgccgac aacattcacc cactcaattt
     5461 gctcaagtga tttgcgaata ccgaacaaga ctatcggatg gtcatcggca ataattacgt
     5521 tcatattgtt catgtaatag gctaccttgc tacagcaagc tcttgacata actgtcaatg
     5581 tcgctgatgt atttttctat tcctggaaca tccttctcac gaatcagatg ttccagcgtt
     5641 tcacataact gcttgccggg taccagattt agcatggcaa atacgccttt aagacgatga
     5701 gccgtttggg ctaacgcagc aaagtcactg gttgctgctt cagtatacag cctcttaaca
     5761 tcatccggta ctgtgtctac aaagagcgca taatagccgc tggcatggag ttgcgcattt
     5821 tcatctccgc ccagaggcga ttctgtcacc tcttcctgcg ccagttgcac ttcaattaat
     5881 tgtaagaccg cttcctgcat agcgttgctc atattgaagt tgacgcacaa ttgaccaggc
     5941 ccaatttccc gtacgccaga ctcatcatcg cttaaaagca agccagaggc agtaagatta
     6001 gacggattat ccgttaaaaa gatatcataa tcttgactaa ttaatctttc atcgggtgtg
     6061 atacaggttg caccccaatt ttctaactgg cgagtgacaa tattccgaat ttctgccgaa
     6121 gtaacatcca ccattacgca gacatcatcc agtaaacgct cttcttcctc ttcaacttcc
     6181 gggccagcgg cgagcatttt gatatgcaca gagtagcgtg taccaagccc atcccgcgtt
     6241 ttgatgttta aatgaccgcc cagtttacgt gccagttgat cgctcagcca gaatgccagc
     6301 gggtccgcct ttccataacg atcgttttgg gtctggttga taaacgggaa gtgcaaatta
     6361 tccatttcat gaatacttac gccttctccg gtgtccagaa tgcggaatgt caggcggtct
     6421 tcagaggact catcctgatc aacctcaagg gtgatttttc ccaattgcgt tgaggtcacg
     6481 gcatattgca tcagtagcag caaaatacgt cgtaaggcat cgcgatcgcc gcggcgcata
     6541 tcgtgtgctt tcagatgatt gttaatcagc agttgcagac ctttacgctt gatggcaggc
     6601 aacactgaag gcacaacttc atcaattaaa tcctgcacgg agaacagcac cgtctcactt
     6661 ttccagctat cgtccgcaag catgttcgct aactgtattt catcgaccag ccgcaccagc
     6721 acatctgcct gattcgccag ttgtttgctt tccggggcgt tgagtttagc cgcgctctcc
     6781 gccagggact gtgcaggttc tttcagcgca tcgccaatgt ttttcataaa ggtcatccgg
     6841 ccctgctggt ttttctcata cagacgctgc gcctgcttga gtttcttgtt taccagcact
     6901 tcgcgatcct gatcgcgaat aatgaaaatt tgtgtgcgcg gcgcgacctg gctgcggaac
     6961 atgcggatct catacagctc gttattgatc gtcgcctgaa taatcccctg atgctgttcc
     7021 gccatggtgg tgatgttttg cagattcaaa tgcggcagca aatgatcggc aattttgtta
     7081 cttatgacag tgcggttcga ttcctgatcg tgaaccagca ggccgagcgg cagcagtgag
     7141 actatctctt cattgattgc ccgtaaaatg cgcaattcgt tattgaccgc cgtgttgggt
     7201 agactttctg tactgcgact ggagaaatgg cggaatgtgg tatagccaaa taacgccagc
     7261 gccagcaaac cgatgttcag cagcagtggc agcagaatgt tttgcaacgt atccagcaat
     7321 aaggtgccat aaggaacctg ccagaccagg cgcatatcgg tagagttgag tgccgaggag
     7381 atttcaatct tcgtactgtt aaagtggata ctgacgctat ccgttccttc tttctcatta
     7441 tcattgtctc ccgttgccgt cgtgtctggc tcaaggcgga aactgtccag cggcataccc
     7501 ggtgggatca aatcattaat cggcagatca aaagccacga ccgttgccag atgtcccggc
     7561 tggttgaacg tggtacgcaa ggtaaagtaa tgaccgttct gccaggccag gcggcgcatg
     7621 ttagaaaaac tttcgcgttc atcgagggcg ttggcctgtt gcaacatttc tgcacgacgt
     7681 gaatcaacaa tgtcactgac ggtcgattct ttaaatccgg aggtgagatc tttgaggggc
     7741 agggttgaga tcagtaccag actgttatcc tgaccattca ggtaatacat cgaccacggt
     7801 acattttctg caccccacaa tgtatccaga taagtggaca tccgctgagt catctcaagc
     7861 gttgagttgt cgtgagagcc aaagattaac gcttccgttt tgcggcgtgg tttctccaga
     7921 tagtagacat cctgtttcag gcgcgtctct tgtaaacctt cgccggagga gggggaggtc
     7981 gtcgcggcaa tgttgtcgta gatctgccag gtcacgtaac gccaggtatc gacgcgcttt
     8041 tggattgcgt gggtaatgtc gacaatctgg taacttttat ctttcagcca ggcgttaacg
     8101 gcgctttgta ccattacccc catcgtcacc agtaacacaa tgatcaacag taaaaagaag
     8161 cgggtaatgc tccccggtag gagtgaaaag cgggtcgtgg ccgttgtctc tttctgacgc
     8221 attcgtgttt atgacctgtt aaaacttcgc gaagtgatag ggcagtataa agggtacagt
     8281 gtgaaattcc agactcttac ctgattgatg attctctatt ctcccgtgat atttatcatc
     8341 ctgcattggg ggaaatagat gtacaagcgc cattttgctg tacaaataac ctacaaaaag
     8401 cccaacgaaa ttaattgcaa ataaacgaag attgctggaa attatgctga tgttaattat
     8461 ttgtgaaata gttgacaaga gttatagttt ttctgtggta gcacagaata atgaaagtgt
     8521 gtaaagaagg gtaaaaaaaa ccggatgcga ggcatccggt tgaaataggg gtaaacagac
     8581 attcagaaat gaatgacggt aataaataaa gttaatgatg atagcgggag atattctagt
     8641 tgcgagtgaa ggttttgttt tgacattcag tgctgtcaaa tacttaagaa taagttattg
     8701 attttaacct tgaattatta ttgct
//
LOCUS       DAESHI010000158         8696 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.160, whole genome shotgun sequence.
ACCESSION   DAESHI010000158 DAESHI010000000
VERSION     DAESHI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8696
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.160"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..134
                     /gene="yhaV"
                     /locus_tag="KW011_003882"
     CDS             <1..134
                     /gene="yhaV"
                     /locus_tag="KW011_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HBH7662105.1"
                     /translation="NDENTLRTYGKKTNAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(189..998)
                     /gene="agaR"
                     /locus_tag="KW011_003883"
     CDS             complement(189..998)
                     /gene="agaR"
                     /locus_tag="KW011_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HBH7662106.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            1247..2527
                     /gene="kbaZ"
                     /locus_tag="KW011_003884"
     CDS             1247..2527
                     /gene="kbaZ"
                     /locus_tag="KW011_003884"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HBH7662107.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCNMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGDAHETLSELAVTTPDAARATLEAHRHAFKKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            2550..3023
                     /gene="agaV"
                     /locus_tag="KW011_003885"
     CDS             2550..3023
                     /gene="agaV"
                     /locus_tag="KW011_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HBH7662108.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            3034..3813
                     /locus_tag="KW011_003886"
     CDS             3034..3813
                     /locus_tag="KW011_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HBH7662109.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPSAQKEEFEDGI"
     gene            3803..4681
                     /locus_tag="KW011_003887"
     CDS             3803..4681
                     /locus_tag="KW011_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBH7662110.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            4699..5133
                     /locus_tag="KW011_003888"
     CDS             4699..5133
                     /locus_tag="KW011_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH7662111.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTCLVDELGRCHEECPVEEGI"
     gene            5130..6263
                     /gene="nagA"
                     /locus_tag="KW011_003889"
     CDS             5130..6263
                     /gene="nagA"
                     /locus_tag="KW011_003889"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBH7662112.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLCITDGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVFDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAAAWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            6614..7768
                     /locus_tag="KW011_003890"
     CDS             6614..7768
                     /locus_tag="KW011_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HBH7662113.1"
                     /translation="MPENYTPAAAATDTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYTPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDVIAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            7781..8641
                     /gene="kbaY"
                     /locus_tag="KW011_003891"
     CDS             7781..8641
                     /gene="kbaY"
                     /locus_tag="KW011_003891"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HBH7662114.1"
                     /translation="MSIISTKYLLQDAQANDYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KCFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         2005 a         2325 c         2314 g         2052 t
ORIGIN      
        1 tgaacgatga aaacactctg cgcacctacg gtaaaaaaac aaatgcctat accgtattca
       61 gcaaaatgtt aaaaagagga catcctcctg ccgactggga aaccctcacc cgagagacag
      121 aagaaaccca ttgatggtgt tcacaatgcc cgccgaacgc attcgtgttg accagcgggc
      181 accctttctc actccccgac cagaatcact tcaaccccag cctttcgcag tccttccaga
      241 ctatccgcag gaatgccttc atcaacaatg atcatgtcga tacgttgagt atcaatgatc
      301 ttatgtaaac tggagcggtt gaacttactg gaatcggtga ccacgatgat ccgttccgca
      361 acttcgcaca tccggcggtt taaacgggct tcatcttcat tatgtgtgct gacgccgcgc
      421 tccagatcga tcgcatctac accaagaaac agcatatcga agtggtaatt ttgcagcgat
      481 tgctcagcct gatcgccgta aaaagattgc gactgacggc gcaaatgccc gccggtcatc
      541 agcagctcaa cgccttcggc ttccagcaac gcattagcca cgttcatacc gttggtcatc
      601 gcaattacgt cagtgtgctt gcgcatcaga cgagcaatct caaaagtggt ggtcccggaa
      661 tcgaggatca cccgatgacc tggctgaatc aactcaacgg cagctttcgc aacgctgcgt
      721 ttcatcgcgg tgttcagtgc gcttttatct tccactgatg gctcgactga cggcgtcgtg
      781 ctatcgcaga tcaacgcgcc accataggca cgcacagcga tcccctgctt ttccagaaac
      841 gccagatcgt tgcggatcgt cacagtagat acgccataca atgccgacag atcgttaacc
      901 tgcacactcc cttgctgtcg caggcgctga atgatctgtt ctcgtcgctc gctggtgcct
      961 gtcactcgct tctcacctga agcgtcggta ttactcatag taagtccttt cgtaaaactt
     1021 tcgtttcatt tcgttttgcc tattaacgcc tttctattaa gcaaatgcaa gcccaccttg
     1081 cccattgacg caagctactc tcgtttcagt aactttcatt atgtttcttt tgtgaatcag
     1141 atcagaaaac tattatcttt cgttttattt ttatctcacc atggcgcagt atcaactgaa
     1201 acaaaacgaa agattaatat cgcagtaatc tgaactggag aggaaagtga aacatctgac
     1261 agaaatggtg agacagcaca aagcgggcaa aacaaatgga atttatgccg tttgttccgc
     1321 acatccgctg gtgctggagg ctgcaatccg ctacgccagt gcaaaccaaa cgccgttact
     1381 gattgaagca acctccaatc aggtagacca gttcggcggt tataccggaa tgacgcccgc
     1441 cgattttcgc ggctttgttt gtcagctcgc cgactcgttg aatttcccgc aggatgcgtt
     1501 gattctgggt ggtgaccatc tggggccaaa ccgctggcaa aacctgccag ccgctcaggc
     1561 aatggccaat gccgatgatt tgattaaaag ctacgttgcg gcaggattca aaaaaatcca
     1621 ccttgattgc aacatgtcct gtcaggacga tccaattccc ttaactgatg acatcgtggc
     1681 tgaacgcgcc gcccgtctgg cgaaagtggc ggaagaaacc tgtcgcgaac actttggcga
     1741 agccgatctg gagtatgtca ttggtaccga agtgccggta cctggcgacg cgcatgaaac
     1801 cttaagcgag ctggcggtca ccacgccgga tgccgcccgc gccacgctgg aagcccatcg
     1861 tcacgccttt aaaaagcagg gtctgaatgc catctggcca cgcatcattg ccctggtggt
     1921 tcaacccggc gtcgaattcg atcacaccaa cgttattgat tatcagcccg ccaaagcgac
     1981 cgccttaagc cagatggtcg aaaactacga aacgctgata ttcgaagcgc actctacaga
     2041 ttatcaaacg ccgcaatcgc tgcgccagct ggtgattgat cactttgcca ttctgaaagt
     2101 tggcccggcg ctgaccttcg ccctgcgtga agccctgttt tctctggcgg cgattgaaga
     2161 agaactggtg ccagcaaaag cctgttccgg tctgcgtcag gtgctggaaa acgtgatgct
     2221 cgaccggccg gaatactggc aaagccacta ccacggtgac ggcaacgcgc gtcgtctggc
     2281 gcgtggttat agctactcgg atcgcgtgcg ctattactgg ccggacagcc agattgatga
     2341 cgctttcgct catctggttc gtaatctggc ggattcacca attccgctac cgctcatcag
     2401 ccagtatctg ccgctgcaat acgtgaaagt tcgctccggc gagctgcaac cgacgccacg
     2461 ggaactcatt atcaaccata ttcaggacat cctggcgcag taccacacag cctgtgaagg
     2521 ccaataagca aaacaaagag gaacacgcta tgccaaatat tgttttaagc cggattgatg
     2581 aacgcttgat tcacggtcag gtcggcgttc agtgggtcgg atttgcgggg gcaaatctgg
     2641 tgctggtagc caacgatgag gttgccgaag atccggtaca acaaaacctg atggaaatgg
     2701 tactggcaga agggatcgcc gtacgtttct ggacgctgca aaaagttatc gacaacattc
     2761 atcgcgccgc cgatcgacag aaaatcctgc tggtttgtaa aacacccgcc gatttcctga
     2821 cgctggtgaa aggtggcgtt ccggtgaatc gcattaacgt tggcaatatg cactacgcca
     2881 atggcaaaca acaaatcgcc aaaacggttt ctgtggatgc gggcgatatc gcagcattta
     2941 acgacctgaa agccgctggg gtggaatgct tcgttcaggg cgtcccgaca gagcctgctg
     3001 tggacctctt taaattactt tgagggattc atcatggaaa tcagtctgtt gcaggcattc
     3061 gcgttgggca ttatcgcctt tatcgctggc ctggatatgt ttaacggcct aacccatatg
     3121 caccgcccgg tggtcctcgg cccgttggtc gggctggtac ttggcgatct gcataccgga
     3181 attttaaccg gcggtacgct ggaactggtg tggatggggc tggccccgct ggcgggcgca
     3241 cagccgccta acgtgattat cggtactatc gtcggcacgg cgtttgccat tactactggc
     3301 gtgaaacccg atgtcgcagt cggtgtcgcc gtacctttcg ctgtcgcagt acagatgggg
     3361 attaccttcc tgttctcggt gatgtctggc gtgatgtctc gctgcgaccg gatggcggag
     3421 aacgccgata ctcgcggcat tgaacgcgtg aactatctgg cactgctggc actcggcact
     3481 ttctattttc tctgcgcttt cctgcctatc tacttcggcg cggaacatgc aaaaaccatc
     3541 attgatgtcc tgccgcaacg attaatcgac ggcctcggcg tcgcgggcgg catcatgcca
     3601 gcaatcggtt ttgccgtgct gctgaaaatc atgatgaaaa acgtctacat cccctacttc
     3661 atcctgggct ttgttgccgc agcctggctc aagttaccgg tgctggctat tgctgccgct
     3721 gcgctggcga tggcgctgat cgacctgctg cgtaaatctc ctgaaccgac tcaaccttcg
     3781 gcacagaaag aggaatttga agatggcatc taatcaaacc accctgccga acgtcagtga
     3841 aaacgaagaa acattgctga ctggcgtcaa tgaaaacgtg tatgaagatc agagcattgg
     3901 cgcggagctg acgaaaaaag atatcaaccg cgtcgcctgg cgttccatgt tgttacaagc
     3961 gtcttttaac tacgaacgta tgcaggcttc tggctggttg tacggtctgc tgcctgctct
     4021 gaaaaagatc cacactaata aacgcgacct ggcgcgcgcc atgaaggggc atatgggttt
     4081 cttcaatacc catccgtttc tggtgacgtt tgttatcggc attatccttg cgatggagcg
     4141 ttctaagcag gacgttaaca gtattcagag caccaaaatt gccgtcggtg cgccgcttgg
     4201 cgggattggc gatgcaatgt tctggctgac gctactgccg atttgtggcg ggataggcgc
     4261 aagcctcgcg ctacaaggct ctattcttgg cgcagtcgtc tttattgtgc tgttcaacgt
     4321 ggtacacctg gggctgcgtt ttggtctggc gcattatgct taccgcatgg gcgtggcggc
     4381 gattccactc attaaagcga ataccaaaaa agtcggccat gctgcgtcaa tcgttggaat
     4441 gacggtaatc ggcgcactgg tggcaaccta tgttcgttta agcaccacgc tggaaatcac
     4501 agcgggcgac gcagtggtta agttacagac tgatgttatt gacaaactga tgcctgcctt
     4561 cttaccgctg gtctacaccc tgaccatgtt ctggctggta cgccgcggct ggagtccgct
     4621 gcgcctgatt gcagtgaccg tggttctcgg catcgtcggt aaattctgcc atttccttta
     4681 aatacaatga ggtattagat gttaagtatt attttgacag ggcatggcgg gtttgccagt
     4741 ggcatggaaa aagcaatgaa gcaaatcctc ggcgatcaat cgcagtttat tgccatcgat
     4801 tttcccgaaa cctcaagcac cgcgctgctt acatcgcagc ttgaagaggc catcgcccaa
     4861 ctggattgtg aagacggcat tgtttttctg acggatttgc tgggcggcac gccgtttcgc
     4921 gtagcttcaa cgctggcgat gcaaaaaccg ggctgtgaag tgattaccgg caccaatttg
     4981 caactgctac tggagatggt gctggagcgc gaagggttaa gcggtgaaga gttccgtgtg
     5041 caggcgctgg agtgtggaca ccgtgggctg acctgtctgg tggacgagtt aggccgctgc
     5101 catgaggaat gtccggtcga ggaaggaata tgacacacgt tctgcgcgcc agaaggctgc
     5161 tgactgaaga gggatggctc gatgaccatc agttgtgtat tactgacggt gtcatcgcag
     5221 caatcgaacc gattccagtg ggcgtgactg aacgcgatgc ggaactgctc tgccctgctt
     5281 acatcgacac ccatgtacac ggtggtgcgg gcgttgatgt aatggatgac gcgccggatg
     5341 tgttcgacaa gctggcaatg cacaaggcac gcgaaggtgt cggcagttgg ttaccgacca
     5401 ccgtaaccgc gccgcttaat accattcatg cggcgctgaa acgtattgct caacgttgcc
     5461 aacgcggcgg acctggtgcg caagtgctgg ggagttatct cgaaggaccg tacttcacgc
     5521 cgcagaataa aggcgcgcat ccgccggagt tgtttcgcga gcttgaaatt gccgagctgg
     5581 atcagttgat tgccgtttct cagcacacct tacgtgtggt agcgctggca ccggaaaaag
     5641 aaggggcatt gcaggccatc cgccatctta aacagcaaaa cgtaagggtg atgctggggc
     5701 atagcgcggc ggcctggcaa caaacccgcg ccgcatttga tgctggtgcc gacggcctgg
     5761 tgcattgcta taacgggatg acaggtttac atcaccgcga accgggaatg gttggcgcgg
     5821 gattaacgga caagcgcgcc tggctggaac tgatagccga tggtcatcat gtgcatccgg
     5881 cggcaatgtc gctgtgttgt tgctgtgcga aagagagaat cgtactgatc accgacgcga
     5941 tgcaggccgc tgggatgccg gatggtcgct atacgttatg tggtgaagaa gtgcagatgc
     6001 acggtggcgt tgtccgtacc gcgtccggtg ggctggcggg cagtacgcta tctgtcgatg
     6061 cagcagtgcg caacatggtc gagttaacgg gcgtaacgcc tgcggaagcc atccatatgg
     6121 cgtcgctgca tccggcgcga atgctgggtg ttgatggtgt tctgggatcg cttaaaccgg
     6181 gcaaacgcgc cagcgtcgtt gcgctggata gcgggctgca tgtgcaacaa atctggattc
     6241 agggtcaatt agcttcgttt tgatggtttg ctccattatt gggccttcac ttcccccgta
     6301 aggcctttct ttttctttcg ttttgatctg tgcagcggtg ccggatgcga cgctaacgcg
     6361 tctgatccga cctacggtac gccgcatccg gcaatcggtg aacatagcga cgtcattacg
     6421 caattcttta atcttccctt gtcttttctt tcaacgatca cagatttcgt tttatttccc
     6481 ttccctccat tgaactttct gtttcttttc tatagatttt aatcaacgaa agacatcacc
     6541 aagtgaaatg aaacgaaagg caagtgaaag cgacaacgcc cgacgtcaag ttcatcagac
     6601 taaggattga gttatgccag aaaattacac ccctgctgcc gccgcaaccg atacatggac
     6661 tgaagaagag atccgccatc agcctcgcgc atggatccgt tcactcacca acatcgacgc
     6721 gctacgttcc gcgctcaata acttccttga accgttactg cgcaaagaga atctgcggat
     6781 catcctgacc ggagccggaa cgtcggcatt tatcggtgac atcatcgcgc cgtggctcgc
     6841 cagccatacc ggtaaaaact tcagcgccgt accgaccacc gatctggtca ccaatccgat
     6901 ggactacctg aatccagctc atccgctgct gttgatctcc ttcggtcgat ccggcaacag
     6961 cccggaaagc gtcgccgccg tggaactggc aaatcaattt gtacccgagt gctatcacct
     7021 gccgatcacc tgcaacgaag cgggcgctct ttaccaaaac gcgatcaaca gcgataacgc
     7081 gtttgccctg ctgatgcccg cagaaacgca cgatcgcggc tttgcgatga ccagcagcat
     7141 taccaccatg atggccagct gcctcgcggt tttcgcacct gagacgatca acagccaaac
     7201 cttccgcgat gtggcggatc gttgccaggc gatcctgacc tcactgggtg atttcagcga
     7261 aggtgtgttt ggttatacac cgtggaaacg gatcgtttat ctcggcagcg gtggcttaca
     7321 gggcgcagca cgcgagtcgg cgctgaaagt gctggaactg actgcgggta aactggcggc
     7381 cttctatgat tccccaaccg gattccgtca tggcccgaaa tcgctggtcg ataacgaaac
     7441 gctggtggtg gtatttgtct caagccaccc ttacacccgt cagtatgatc ttgatctgct
     7501 ggctgaactt cgccgtgaca accaggcaat gcgcgtaatc gccatcgccg cggaaagcac
     7561 cgacgttatc gccgctggcc cgcatatcat cctgccgcca tcccgtcact ttatcgacgt
     7621 tgagcaggca ttttgcttcc tgatgtacgc ccagacgttt gcactgatgc agtcgctgca
     7681 catgggcaat acgccggata ccccatcagc cagtggcacc gttaaccgcg tggtgcaagg
     7741 cgtaatcatt catccgtggc aggcataaga ggatcgcatt atgagcatta tctccaccaa
     7801 atatctgtta caggacgccc aggccaatga ctacgcggtg cctgccttta acattcataa
     7861 cgccgagacg atccaggcga tcctcgaagt gtgcagtgaa atgcgatcgc cggtgatcct
     7921 cgccggaacg ccggggacct ttaaacatat cgcgctggaa gagatctacg ccctgtgtag
     7981 cgcctattcc acaacctaca acatgccact ggcgctgcat ctcgaccacc acgaatcgct
     8041 ggatgatatt cgccgtaaag tccacgcagg cgtgcgcagt gcgatgatcg acggcagcca
     8101 cttcccgttt gccgagaacg taaagctggt gaaatcggtt gtcgacttct gccactccca
     8161 ggattgcagc gtggaagcag aactgggccg cctgggcggt gttgaagatg acatgagcgt
     8221 tgacgccgaa agtgcattcc tgaccgatcc acaagaagct aaatgctttg tcgaactgac
     8281 tggcgtcgac agcctggcgg tagcgattgg tacggcgcac ggcttataca gcaaaacgcc
     8341 gaagattgat ttccagcggc tggcggaaat tcgtgaagtg gtggatgttc ctctggtgct
     8401 gcatggtgcc agcgatgttc cggatgaatt tgtccgtcgc actattgaac ttggcgtcac
     8461 aaaagtgaac gttgccacag aattaaaaat agccttcgct ggcgcggtta aagcctggtt
     8521 tgcggaaaat ccgcagggta atgatcctcg ttattatatg cgcgtcggaa tggatgcgat
     8581 gaaagaagtt gtcagaaata aaattaatgt ctgtggttca gcgaatcgaa tttcagcata
     8641 atcattagtt ttttatgatt tatcccaatg tacttcccga ttaatatcgg ggagtg
//
LOCUS       DAESHI010000159         8632 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.161, whole genome shotgun sequence.
ACCESSION   DAESHI010000159 DAESHI010000000
VERSION     DAESHI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8632
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.161"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(143..2686)
                     /gene="mdoH"
                     /locus_tag="KW011_003892"
     CDS             complement(143..2686)
                     /gene="mdoH"
                     /locus_tag="KW011_003892"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HBH7662115.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2679..4214)
                     /gene="mdoG"
                     /locus_tag="KW011_003893"
     CDS             complement(2679..4214)
                     /gene="mdoG"
                     /locus_tag="KW011_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HBH7662116.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4608..5765
                     /gene="mdoC"
                     /locus_tag="KW011_003894"
     CDS             4608..5765
                     /gene="mdoC"
                     /locus_tag="KW011_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HBH7662117.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5773..7254)
                     /gene="clsC"
                     /locus_tag="KW011_003895"
     CDS             complement(5773..7254)
                     /gene="clsC"
                     /locus_tag="KW011_003895"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HBH7662118.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7196..7729)
                     /gene="ymdB"
                     /locus_tag="KW011_003896"
     CDS             complement(7196..7729)
                     /gene="ymdB"
                     /locus_tag="KW011_003896"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HBH7662119.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7824..8135)
                     /locus_tag="KW011_003897"
     CDS             complement(7824..8135)
                     /locus_tag="KW011_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBH7662120.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8256..8588)
                     /gene="csgC"
                     /locus_tag="KW011_003898"
     CDS             complement(8256..8588)
                     /gene="csgC"
                     /locus_tag="KW011_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HBH7662121.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
BASE COUNT         2155 a         2246 c         2051 g         2180 t
ORIGIN      
        1 ataacgccgg tatttttatt ggctcgttgt ttttgccgga tgcgacgtga acgctttacc
       61 taacccacaa aagcttgcaa aatcaataaa ttgcatgaac gatgtaggcc tgataagcgt
      121 agcgcatcag gcaactacat ttttattgcg aagccgcatc cggtttacgc aatgccagtg
      181 gattgagctt tatcccttcg taataactca cccatgaaga atatctctca ggggaattcc
      241 agacgcggaa atgcagacgg gccatcgtca ccggatcgct tagcagcaca aggcgacgat
      301 cgcgattcag cttctctggc gtctcgttca gcgcctgttc aacgtggcgg tcacgggcga
      361 tttccagcac cttactggcg cggtgacgcg cggtcgccat tgcggttgcc agagcgttaa
      421 atgacgggtt aaacactgcg tgcataaagc catcgtcaag ggagcgttgg cgattcatct
      481 caaggaaccg atcggtatca accagcacct gcggcggtga atactcttcc gggatcagga
      541 acagtttcca gcgtttggtg cgcagaccaa cggtggcacg gctggaaatc accgaaacaa
      601 acggtgacag gatcaacgag aagacaatcg gtgccagcca gaacaggaaa cgcagatcca
      661 gccacgccat cccaacagcc cacactaacc ctaacagcag ctgtgagccg tggcgtttga
      721 acgcttcacc ccaggaagtg gagtcatcat cacgctgcgg tgaattccac accacttccc
      781 agccaaggaa cgcgctgacg acgaagaccg tatggaacag catgcgtacc ggagccagca
      841 gcacggaaaa cagcacttcc agcagcaacg ataatgtaac gcgccagaag ccgccgtatt
      901 ctttcgttcc tttgcaccag ataagcaaaa tgctcaataa cttcggcagg aacaacagca
      961 ccatggtcga agcaaaaagt gcaatcgcca gctcaggacg ccactgcggc cacaccggga
     1021 acaactgccg tggttgcagg aagtattgcg gttcggtcaa cgcatgtact acctgcaatg
     1081 cagtagagag cgcgaggaac ataaaccaca gcggagcgga gagataagac atcacgcccg
     1141 tcaggaacac cgcacggtga accgggtgca tacccttcac caggaacaga cggaagttca
     1201 tcaggttacc gtggcaccat cggcggtcac gttttagctc atcaagcaag ttaggcggca
     1261 attcttcata agaacccggg agatcgtaag caatccagac cccccaacct gcacggcgca
     1321 tcaacgccgc ttccacgaag tcatgtgaca ggattgaacc ggcaaaggaa ccttcgcccg
     1381 gcagcggagc cagtgcgcag tgctcgataa acggtttcac gcggataatc gcgttatgtc
     1441 cccagtagtg cgactcgcca agttgccaga agtgcaaacc ggctgtaaat agtggcccat
     1501 acacgcgggt cgcgaactgc tgacagcgcg catacagcgt atccataccg gacgctttcg
     1561 gcgacgactg aatgatcccg gcgttcgggt tggcttccat caggcgcacc agcccgcaca
     1621 aacaatcacc ggtcattacc gagtcagcat ccagcaccac catgtagctg tactggctgc
     1681 cccagcgacg gcagaagtca tcgatattac cgcttttacg cttcacgcgg cgacggcggc
     1741 ggcgatagaa aatctgacct tcgccaccga cttcagcgat aagctccatc caggcttttt
     1801 gctctgcgac gcagatatcc gggttataac tgtcactaag aatgtagaca tcaaagtgtt
     1861 tggcattccc ggtggctttt actgattccc acgttgcacg caggccagca aaaacacggt
     1921 tcacgtcttc gttacagata ggcatgatca acgccgtgcg atgctccggg tttaatggtt
     1981 catcgccaac tgttgacgca gatatactgt atttatcgcg accaataagc agttgcagga
     2041 agcccattaa cgccgtccag aatccggcgg acacccaaca gaacagtacc gcaaagagga
     2101 tcaggatacc ggtttgcagc atataaggca gaagctgcat aaaggaaacc cacaaatcct
     2161 gaccaaccat atccatagga ttaatcagcg cccaaccctg ataaggaaga atggtcttca
     2221 tataccaggt cgcgacgaca gtttgcgcga gcgtcaggat caacagaatg taacggcgga
     2281 tggtaccgac ggtacgccac ttttgctcgc tctcctgctc ttctttggtc aaacgagcca
     2341 gatagcgcgg cgtgacatcg cgtccacgca ggcgatccca gaaacggcct accgggttgg
     2401 tacgccacgg gtcgggaaac atcgaggagc gttttgcttc tggcatcgcc ttcagctgat
     2461 cgcgcccttc gtcgtcttta attaactgtc catcagcaag tgaatctggc caggcttgtt
     2521 ccagacgcgc ctttaccgag ccttgcgggg aatcatcctc ccgcgcccag gtgcggtgtt
     2581 cggcatccag cgcctgatga acggcgcgga tatcagtctt cggcaatgcc gctttctcgc
     2641 ttgcggcgat gggcattgcg tcaatgtact cagttgtctt attcattggc aggtaactgg
     2701 tagctccagg tttcactcaa cgtctgatcg gcattcacca gcgcagcacg catttcagtg
     2761 gttttcttgg catctttcac tttcacacgc atcaccagac gccagccttt ggtaaccggg
     2821 ttatagcgca ccgtgctttc aactatctca ccattatcac caatgctggt ttgcgctgtg
     2881 accggggtat cctctggcag ttttttcatc tctgcgccgg taaaatcgac cacaaaggcg
     2941 atagtaccgt caggctggcg aatcaggttc gactgcttca catcccccgt tgaacgacgc
     3001 gtttgttgca cccatgcgtt atctggcgca tgcagtttgt cttcatcacg gctgaaggtg
     3061 atggtgtatt taaagttcat ctctttaccc ggctccggca gctgatccgg cgtccagtaa
     3121 gcgacgatgt tatcgttggt ttcatcgttg gttggaattt ccaccagctc aacgctgcct
     3181 ttaccccact cccctttcgg agtcacccat gcgcttggac gaagatcgta acgatcatcg
     3241 agatcttcaa agcgggagaa atcacgaccg cgctgcaaca gaccgaagcc ttgcgggttt
     3301 tccatcgaga agctgctaac cgctaaatgt ttcgggttat tcaacggacg ccagatccat
     3361 tcgccattac cggcatggat agagagaccg ttagagtcgt gcaactccgg acgatagtta
     3421 tttgcaggcg acggttggtt cggcccaaac aggaacatac tggttaacgg tgcaaccccc
     3481 agtttgccga ctttatcgcg cagatagatt ttcgactgca catccacaac cgtgtcacgc
     3541 cctggcataa ccacgaattt gtaagcacct gtcgcgcgcg gcgagtcaag caatgcataa
     3601 atggttaaac gtttatcagt cggttttgga cgctcgatcc agaactcttt gaagcgtgga
     3661 aattcttccc ccgatggcaa ggcggtatca attgccaggc cgcgggcaga aaggccataa
     3721 acctgacctg caccaatcac gcggaaatag ctggccccga gcatgctgac gatttcatcg
     3781 tttttatctt tgctgttgat cgggtaaagc actttaaaac cggcaaaacc aaggtctttt
     3841 accgtgtctt tgtcatgctg aacatcgccg aaagtgaaat aatccgggct gtatttgatt
     3901 cgtttgactg cggtggcagt cacttcattt attttgaccg gggtatcgaa gtacataccc
     3961 tgatggtaga actcgagttt gaatggggtc ttcagattgt tccagtacgc tttgtcatga
     4021 ttaaactgga tctgctgata gtccgcgtat ttcatatcgc ggaaaacgga gggcaagttg
     4081 cttttgggcg cctcatagcc tttcccggct aaggattgag cttgctttgc gacatcatca
     4141 atactgaaag cccagcttga agatgtatac agggttaaca ttactgcagc actcaaccaa
     4201 cgcattttca tcatttgtag tttatgtttc ataattagta agcacttccc cctttgtgtg
     4261 cttatatcga tccgatctat tttaatggat aaccagccat tccgacaaca gaatcccggt
     4321 atcgttcagc acgctggctc atggattaag cacctgattg caaccgtttt cactttccgt
     4381 gcttctccta aagacagaag catcgacttc gtgtagggtt tgccagcaat tttatttatc
     4441 aaccgtctta cagacgcttc cattctggtt agtggtagcg atgatgctat gcataaaaac
     4501 cttgaagcct gcgcctggcg cggtcaggtc ggtgtcgcta tggcaacaag cctaaaggca
     4561 taacccgaca gggataagac gaaaaatccg agattagtta accatatatg aatccagtac
     4621 ccgcgcaacg tgaatatttc ctcgactcca tccgcgcctg gctgatgttg ttagggatcc
     4681 cttttcatat ttctttaatc tattcgagcc atacatggca tgtgaatagc gccgaaccgt
     4741 cattgtggct gacccttttt aatgacttca tccactcgtt ccgcatgcag gtatttttcg
     4801 ttatatccgg ctacttttcc tacatgcttt ttttacgcta tcccttgaaa aaatggtgga
     4861 aagtacgtgt cgaacgtgta ggtatcccga tgttaacagc catcccctta ctgacattgc
     4921 cgcaatttat tatgctgcaa tacgtcaaag ggaaagcgga aagttggcct gggctgtcat
     4981 tgtatgacaa atataatacg ttggcctggg aattaatatc acacctgtgg tttttactgg
     5041 tgttagtagt catgacgacg ctgtgcgtat ggatatttaa acgcatcaga aataatttag
     5101 aaaattctga taaaacgaat aaaaaattct cgatggtaaa actatcggtg atttttttat
     5161 gcctcggcat cggctatgcg gtaataagaa gaacgatttt tattgtgtat ccgcccattc
     5221 tgagtaatgg catgttcaat tttattgtca tgcaaacgct attttatttg ccgttcttta
     5281 tcctcggtgc actggctttc attttccctc atcttaaagc cttgtttacc acgccgtctc
     5341 gtggctgtac ccttgcagca gcattggcat ttgtcgctta tttactcaac cagcgctatg
     5401 gcagtggcga tgcctggatg tacgaaaccg agtcggtgat caccatggtc ctcggtctgt
     5461 ggatggtgaa tgtggtcttc tcctttggcc accgtttgct taacttccag tcagcgcggg
     5521 tgacttactt tgttaacgca tcgctattta tctatctggt tcaccacccg ttaacgctgt
     5581 ttttcggcgc atacattaca ccgcacatca cctccaactg gcttggtttt ctctgtggcc
     5641 tgatattcgt agtagggatt gcgataattc tgtatgaaat tcatttacgc atcccgttac
     5701 tgaagttttt gttttctggt aaaccggttg ttaagcgtga gaacgataaa gcaccagccc
     5761 gttaagccac atttacaata accattccac gggcaatatc gacgccagtc tgaccataac
     5821 ccgcttccag aaactggtgg cgggttcttt tttgagaata atctctttct ttgcatgacg
     5881 atcaacccag ttgatccgtc cccacctgtc cagacggagc tgccaggccg catcatactg
     5941 gctctgaata aagcgtttat caattaactg tgccagcgtt tcgctctcta tcacgaagcc
     6001 catttcagta ttgagcaatg ttgaacgcgg atcgaaattg aaagagccga taaacaccgt
     6061 tttaccatcg atgctaaaag ttttagcatg caggctggct ccggaattac cggttatgcc
     6121 gcgatcgtgt aacgtactac tttgttcacg cgtcggcttg agttcatata attccacgcc
     6181 atagcggagc aattttttgc gccagcgcgc gtatccggca tggacgacag caacatcgtt
     6241 agcggcaaga gaattggtta ggatcgcaat ctttacccct tttctcacca tccgtaagag
     6301 ttgcgccaca cctgcgcgtg tcggtacaaa ataggaagag ataatatcga tgcgttcact
     6361 gggtgagccc atgatatcgg acaggcgctg cggtaacagt gaatgccgtt ttgccttgcc
     6421 ctcccctttc gccggatcat cacttaataa acgtgttttc gcccagataa gcggcaacgt
     6481 tccatcaacc agatgattta taaacggact ggattccatt tggcgtaagt aacgatgcgt
     6541 catggcatcg ttatgccagg aggcgggtaa ctcgatgcga tccgccattt caccttccgg
     6601 gacatccagc acctgctgta agggtgaaac cgatttgcaa taccagtagc gggcgaaatc
     6661 atcggcaacg tcctctacca cgggtcctat tgccataaca tctaaatccg aaaaaagtgg
     6721 ctcttcccct gccccaaaat aggcatcacc aatatttcgt cctcccacca gggtaaccac
     6781 gccatcgaca gtgaaacttt tattgtgcat acggcgattg agacgggaaa agtcggtgat
     6841 ataaccaagc ggacgcagca agcgaaacga gaaaggatta aaaagccgga cttcaatgcg
     6901 gggatggctg tcaagcaagc gtaaaatatc gtcaagtccg ggcgtattgt tgtcgtccag
     6961 caacaaacgg acgcgaacgc cacgctttgc tgcggctaac agggcggaaa acagtaaccg
     7021 acccgacata tcgtcctgcc agatgtaata ctgaacatcc agcgtatgct ctgccatttc
     7081 ggccagacga taccgggcgg caaacgcatc cagacttttc tccagaggga aaagaccaca
     7141 ctgaccggga tgttgcgaac acagtggcag caccgcgctc gccagccggg gcaaatcatt
     7201 catctccttg ttgggtaagg agtctttcgt agaggtgggc gttttcttca tcataacaga
     7261 caaagtatac ctgttcgggt aaagcgtgac gggtaataaa ttctgaaacg gttttaactg
     7321 cgatttcagc cgctgccgca cgagggtaac cataaacccc agtactgatt gcaggaaaag
     7381 ccactgacgt atagctgttt gccgccacca gtcgcaggct attcagatag gcatcctgca
     7441 aaagctggtc ttcgttttgt tcaccaccac gccagactgg cccgacggtg tgtactacgg
     7501 ctttagcggg aagatcgcct gcaagcgtaa taacggcatg ccccgtaggg caatcgccct
     7561 gctgttgcct gacttttaaa caggcatcca gcagggccgg acccgctgcg cgatgaatgg
     7621 ccccatcgac gccgccgcct cccattaatg acggattagc cgcattcaca atcacatcaa
     7681 cggccagttt ggtaatatca ccctgcacaa catgaatacg cgttttcata acgcctcctt
     7741 acaccttctt gtttcttaag tgtacagcag gctttttggt taaatcgcat ttttctgctc
     7801 agcaacgtgc gttgttcaga aacttagtta tattcgatgt tcattacagc cagctttttc
     7861 tgtttatcaa gatactgcac ttttactgag gctatttgcg cgtttttaac attaccgctc
     7921 attaacgctt tgctggaata ggttttgccc ggaacagaac cattctgtgg gcagttcatt
     7981 tctacgggtg atgactgggt gctgacgtca catgccggtt ccacaatttg gccataaaaa
     8041 tggatcacac ccccttgcgc ggtgcttcct gcgatggcaa taaaaggaaa aaacaaactg
     8101 ccgagcataa gagagtttaa aaaaggtctg aacatttatt ctatttcctc aatgattagt
     8161 catccttgag ggttgtgtta tccatacttt caggttggct gattcgcagc agaccattct
     8221 ctccagattc atcttatgct cgatatttca acaaattaag acttttctga agagggcggc
     8281 cattgttgtg ataaatgaag tgcctgtcca tcagaaacag taacaactat tttcacccga
     8341 tcttccgggg aaatatttaa actcaacttc gtcaaagcaa taggttgatt agcaggcaat
     8401 gagagggtct tttcttgctt cgtctgactt tgccctgaac tgccttcgcg cagggacaat
     8461 atttgtactc tgcacagaca agattgagta agagtgactt caggaataat ggtatacaca
     8521 tccccttgct gggtcgtatt aaaggttatc tggctggaaa gtgccgcaag gagtaataac
     8581 gcattcatat tcttctcccg aaaaaaacag ggcttgcgcc ctgtttctgt aa
//
LOCUS       DAESHI010000160         8452 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.162, whole genome shotgun sequence.
ACCESSION   DAESHI010000160 DAESHI010000000
VERSION     DAESHI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8452
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.162"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="KW011_003899"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="KW011_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HBH7662122.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="KW011_003900"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="KW011_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HBH7662123.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="KW011_003901"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="KW011_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HBH7662124.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="KW011_003902"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="KW011_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HBH7662125.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="KW011_003903"
     CDS             complement(4036..5568)
                     /locus_tag="KW011_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HBH7662126.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /gene="dadA"
                     /locus_tag="KW011_003904"
     CDS             5898..7196
                     /gene="dadA"
                     /locus_tag="KW011_003904"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HBH7662127.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7206..8276
                     /gene="dadX"
                     /locus_tag="KW011_003905"
     CDS             7206..8276
                     /gene="dadX"
                     /locus_tag="KW011_003905"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HBH7662128.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
BASE COUNT         2115 a         2129 c         2268 g         1940 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 accgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgagg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatccggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatagcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc cgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aattagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggtacgc
     8161 cggttgagct gtggggcaag gagatcaaaa ttgatgatgt cgccgccgct gccggaacgg
     8221 tgggctatga gttgatgtgc gcgctggtgc tacgcgtccc ggttgtgacg gtgtaacttg
     8281 ttgtaagccg gatcggaggc aacgtcttct gggtgcaaaa aaaatcatcc atccggctgg
     8341 tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaagg
     8401 atggacacta tttccccgga acctgaactc accgcacagg cgttctacat aa
//
LOCUS       DAESHI010000161         8214 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.163, whole genome shotgun sequence.
ACCESSION   DAESHI010000161 DAESHI010000000
VERSION     DAESHI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8214
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.163"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="KW011_003906"
     CDS             complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="KW011_003906"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HBH7662129.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1360..1752)
                     /gene="ydeI"
                     /locus_tag="KW011_003907"
     CDS             complement(1360..1752)
                     /gene="ydeI"
                     /locus_tag="KW011_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HBH7662130.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            2028..2546
                     /locus_tag="KW011_003908"
     CDS             2028..2546
                     /locus_tag="KW011_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HBH7662131.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(2591..4636)
                     /gene="dcp"
                     /locus_tag="KW011_003909"
     CDS             complement(2591..4636)
                     /gene="dcp"
                     /locus_tag="KW011_003909"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HBH7662132.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            4773..5519
                     /gene="ydfG"
                     /locus_tag="KW011_003910"
     CDS             4773..5519
                     /gene="ydfG"
                     /locus_tag="KW011_003910"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HBH7662133.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5608..6294
                     /gene="ydfH"
                     /locus_tag="KW011_003911"
     CDS             5608..6294
                     /gene="ydfH"
                     /locus_tag="KW011_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HBH7662134.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            6471..6674
                     /gene="ydfZ"
                     /locus_tag="KW011_003912"
     CDS             6471..6674
                     /gene="ydfZ"
                     /locus_tag="KW011_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HBH7662135.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(6710..8170)
                     /locus_tag="KW011_003913"
     CDS             complement(6710..8170)
                     /locus_tag="KW011_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBH7662136.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
BASE COUNT         2171 a         2055 c         1870 g         2118 t
ORIGIN      
        1 cggagataat ccctcaccta accggcccct tgttacagtt gtgtacaagg ggcctgattt
       61 ttatgacggc gaaaaaaaac cgccagtaaa ccggcggtga atgcttgcat ggatagattt
      121 gtgttttgct tttacgctaa caggcatttt cctgcactga taacggatcg ttgacacagt
      181 agcatcagtt ttctcaatga atgttaaacg gagcttaaac tcggttaatc acattttgtt
      241 cgtcaataaa catgcagcga tttcttccgg tttgcttacc ctcatacatt gcccggtctg
      301 ctcttccaat gaccacatcc agaggctctt caggaaatgc gcgactcaca cctgctgtca
      361 cggtaatgtt gatatgccct tcagaatgtg tgatggcatg gttatcgact aactggcaaa
      421 ttctgacacc tgcacgacat gcttcttcat cattagccgc tttaacaata atgataaatt
      481 cttcgccccc gtagcgataa accgtttcgt aatcacgcgt ccaactggct aagtaagttg
      541 ccagggtgcg taatactaca tcgccgatta aatgcccgta ggtatcatta accaatttaa
      601 atcggtcaat atccaacaac attaaataaa gattcagagg atcagtgttg cgtaactgat
      661 gatcaaagga ttcatcaaga acccgacgcc ccggcaatcc cgtcaaaaca tccatattgc
      721 tacggatcgt cagcaaataa attttgtaat cggttaatgc cgcagtaaaa gaaagcaacc
      781 cctcctgaaa ggcgtcgaaa tgcgcgtcct gccagtgatt ttcaacaata gccagcatta
      841 attcccgacc acagttatgc atatgttgat gggcagaatc cattagccga acgtaaggta
      901 attcatcgtt atcgagtggc cccagatgat caatccaccg accaaactgg cacagtccat
      961 aagaatggtt atccgttatt tctggcttac tggcatctct cgcgaccacg ctgtgaaaca
     1021 tactcaccag ccactggtag tgggcatcga tagccttatt gagatttaac aagatggcat
     1081 caatttccgt tgtcttcttg atcattgcca ctcctttttc acagttcctt gtgcgcgcta
     1141 ttctaacgag agaaaagcaa aattacgtca atattttcat agaaatccga agttatgagt
     1201 catctctgag ataacattgt gatttaaaac aaaatcagcg gataaaaaag tgtttaattc
     1261 tgtaaatcac ctctgcatta tcgtaaataa aaggatgaca aatagcataa cccaataccc
     1321 taatggccca gtagttcagg ccatcaggct aatttatttt tatttctgca aatgagtgac
     1381 ccgaacgacg gccggcgcgc ttttcttatc cagactgccg ctaatgttga tcatctggtc
     1441 cggctgaact tctcgtccat caaagacggc cgcaggaata acaacattaa tttcaccgct
     1501 cttatcgcga aaaacgtaac ggtcctctcc tttgtgagaa atcaaattac cgcgtagtga
     1561 aaccgaagcg ccatcgtgca tgttttttgc gaaatcaacg gtcatttttt ttgcatcatc
     1621 ggttccgcga tagccatctt ctattgcatg aggcggcggt ggcgctgcat cctgttttaa
     1681 accgccctgg tcatctgcca acgcataagg catgacaaga aaacttgcta atacaatgga
     1741 ctgaaatttc atactaactc cttaattgcg tttggtttga cttattaagt ctggttgcta
     1801 tttttataat tgccaaataa gaatactgcc aattgttata aggcatttaa aatcagccca
     1861 ctagctgtca aatattcaga gaatttgact cactaaagtt aagaagattg aagagtctta
     1921 aacatgtttt cagaataatc ggatttatat gtttgaaaat tattatattg gacgagcata
     1981 cagaaaaagc aaatcacctt tacatataat agcgtggaca aaaaacagtg aacattaata
     2041 aagataaaat tgtacaactc gcagataccg acactattga aaacctgaca tccgcgttga
     2101 gtcaaagact tatcgcggat caattacgct taactaccgc cgaatcatgc accggcggta
     2161 agttggctag cgccctgtgt gcagctgaag atacacccaa attttacggt gcaggctttg
     2221 ttactttcac cgatcaggca aagatgaaaa tcctcagcgt aagccagcag tctcttgaac
     2281 gatattctgc ggtgagtgag aaagtggcag cagaaatggc aacaggtgcc atagagcgtg
     2341 cggatgctga tgtcagtatt gccattaccg gctacggcgg accggagggc ggtgaagatg
     2401 gtacgccagc gggtaccgtc tggttcgcgt ggcatattaa aggccagaac tacactgcgg
     2461 ttatgcattt tgctggcgac tgcgaaacgg tattagcttt agcggtgagg tttgccctcg
     2521 cccagctgct gcaattactg ctataaccag gctggcctgg cgatatcccc aggccagcca
     2581 ttggtggtgt ttatatgttc aagccacgat gttgcagcat cggcataatc tgaggagcct
     2641 taccgcgcca ttgtcgatgc aggcgttcca gatcttcgct gttacctctg gaaaggatcg
     2701 cctcgcgaaa acgctgccca ttttcacgcg ttaatccgcc ctgctcaaca aaccactggt
     2761 aaccatcatc ggccaacatt tgcgtccaca gataagcgta ataacctgca gcatatccgc
     2821 caccaaaaat atgggcgaaa taactgctgc gatagcgtgg cggtatagca ggaagatcca
     2881 tattttccgc caccagcgcc cgcaattcaa aatcatcgac atcctgcatt gcttcgtttt
     2941 cttccaggca atgccagcgc atatcgagaa gtgcggcgct aagcagttcg ctcatctcat
     3001 accctttgtt gaacaggctg gcattacgca ttttctgttg cagttcgtca ggcattgctg
     3061 ccccgctctg ataatgccgg gcgtagcgag cgaatacctg cggatgcgtt gcccagtgtt
     3121 cgttgatttg cgacggaaat tcgacaaaat cacgcggcgt gttggtgccg gaaagcgtgg
     3181 cataacgctg gcgggcaaaa aggccgtgca gcgtatgacc aaattcatgg aataaggtta
     3241 ttacatcatc ccagagtaac aacgcaggct caccggcagc gggtttctga taattgcaga
     3301 cgttataaat taccggatgc gtttcattaa gcgttgattg ctcaacaaaa ttgcccatcc
     3361 atgcaccgcc gctttttgaa tcacgggcga agaaatcacc gtaaaataac gccagcccca
     3421 cgccattatg atcaaaaatt tcccacaccc gaacgtcagg atggtagaca ggaatatcaa
     3481 aacgttcgac aaacttaata ccgaagagct gattcgcggt ccagaataca ccttcattca
     3541 acaccgtgtt taattcaaaa tatggcttga gctgcgcttc atcaagatca aatttctccc
     3601 gccgtacctg ttccgcataa aatgcccagt cccacggctg cgcgctaaac ccgccctgct
     3661 gcttatcgat aaccgcctgt atggaggcta attcatcgct cgcacgttga cgcgccgctg
     3721 gaacaatttc ccgcataaag ttgagtgctg cttctggcgt ttttgccatc tgatcggcga
     3781 ttttccatgc ggcataatga ggaaaaccaa gcagtttcgc ctgctgtgcg cgaatctcta
     3841 ccagacgttg aatgatagcg cgggtatcat tgccatcatt tttttccgct cgcgtccagc
     3901 ccgcaataaa cagtttttca cgcgtcgcac gatcgcgcat ttcggcaagc gccggttgtt
     3961 gggtggtatt cagcagcgga atcagccatt tgttatccag acctttctcg cgagccgcct
     4021 ctgccgccag cgcaatctct tgctcactca ttcctgccag ctgcgcgata tcgttcacaa
     4081 ccagaccgcc ggatttattt gctgccagta atcgctggtt aaactggctg gtcagggtcg
     4141 cagcttctgt attcagtact tttaattttg ctttatcagc ttgcgcaagt ttggctccgg
     4201 caaggacaaa acgttgatga atcacctcca ccaggcggat ggattcacta tcaagcccca
     4261 gggattcacg gcgctgccag acagcatcta cccgcgcgaa taattcaccg ttcagataga
     4321 tatcattagc cagttccgcc agttcagcgg aaaactgttc atcaagacgc tgtaattcat
     4381 cattggtatg cgccgcagtc atcgcaaaaa agacgctggt aacgcgggta agtaattctc
     4441 cgctttgttc cagtgccaga atagtattgt tgaaatcagg cgtttgcggg ttaagcgcga
     4501 tggcagcaat ttctgcccgc ttttgctgca ttccctcatc gaatgccggg cgatagtgat
     4561 gattggcaat ttgatcaaaa tggggagcca gatacggtag tgtgctttgc acaaggaaag
     4621 gattcattgt tgtcattttc ttctcctgaa cgcgagatgt tccatagcgt aggcttactg
     4681 ataacggagt gcaatcttgc aaaccagtat tacccgctct taagcatcac gtgctatgtt
     4741 attgacacac aaaagcgttg aggaacagtg agatgatcgt tttagtaact ggagcaacgg
     4801 caggttttgg tgaatgcatt actcgtcgtt ttattcaaca agggcataaa gttatcgcca
     4861 ctggccgtcg ccaggagcgg ttgcaggagt taaaagacga actgggagat aatctgtata
     4921 tcgcccaact ggacgttcgc aaccgcgccg ctattgaaga gatgctggca tcgcttcctg
     4981 ccgagtggtg caatattgat atcctagtaa ataatgccgg cctggcgttg ggcatggagc
     5041 ctgcgcataa agccagcgtt gaagactggg aaacgatgat tgataccaac aacaaaggcc
     5101 tggtatatat gacgcgcgcc gtcttaccgg gtatggttga acgtaatcat ggtcatatta
     5161 ttaacattgg ctcaacggca ggtagctggc cgtatgccgg tggtaacgtt tacggtgcga
     5221 cgaaagcgtt tgttcgtcag tttagcctga atctgcgtac ggacctgcat ggtacggcgg
     5281 tgcgcgtcac cgacatcgaa ccgggtctgg tgggtggcac cgagttttcc aatgtccgct
     5341 ttaaaggcga tgacggtaaa gcggaaaaaa cctatcaaaa taccgttgca ttgacgccag
     5401 aagatgtcag cgaagccgtc tggtgggtgt caacgctgcc tgctcacgtc aatatcaata
     5461 ccctggaaat gatgccggtt acccaaagct atgccggact gaatgtccac cgtcagtaat
     5521 ttttataccc ggcgtaactg ccgggttatt gcttgtcaca aaaaagtggt agactcatgc
     5581 agttaactca ctcacaagca agaacgaatg accgtcgaaa cgcaacttaa tcccacacag
     5641 cctgtcaatc agcaaattta tcgtattctt cgtcgcgaca ttgtccattg cctgattgca
     5701 ccaggcacac cgttgtcgga aaaagaagtt tctgttcgtt tcaatgtgtc acgccagccg
     5761 gttcgtgaag cctttattaa actggcggaa aacggcctga ttcaaattcg tccgcagcgt
     5821 ggcagctacg tcaacaaaat ttccatggcc caggtgcgca atggcagttt tatccgtcag
     5881 gccattgagt gcgcggtggc gcgtcgggcg gcgagcatga ttaccgaaag ccagtgctac
     5941 caactggaac aaaatcttca ccagcaacgc attgccattg agcgcaagca actggatgat
     6001 ttttttgaac ttgatgacaa cttccatcaa ctcctgacgc agattgccga ctgtcaactg
     6061 gcgtgggata ccattgagaa cctgaaagcg accgttgatc gcgtgcgcta tatgagtttc
     6121 gaccacgttt ctccaccaga aatgctgtta cgccagcatc ttgatatttt ctctgccctg
     6181 caaaaacgtg atggcgatgc ggtagaacgt gcaatgacgc aacatttgca ggaaatcagc
     6241 gaatccgtgc gccagatccg ccaggaaaat agcgactggt ttagcgaaga gtaattcatt
     6301 tcctctcatc ccatccgggg tgagagtctt ttcccccgcc ttatggctca tgcatgtatc
     6361 aaaaaagatg tgagcttgat caaaaacaaa aaatatttca ctcgacagga gtatttatat
     6421 tgcgcccgtt acgtgggctt cgactgtaaa tcagaaagga gaaaatacct atgacgacct
     6481 acgatcgtaa ccgtaacgca atcaccactg gcagccgtgt tatggttagc ggcaccggtc
     6541 acactggcaa gatcctgtcg attgatactg aaggtctgac cgctgagcaa atccgccgcg
     6601 gaaaaaccgt agttgttgaa ggttgtgaag agaaactggc acctctggac ctgattcgtc
     6661 tcggcatgaa ctaagcgtgt gaatgccgcc gatggcggca ttgctttttt tacttcacgg
     6721 aatatttcgc cacggtcgct ttcgcgccat gcgctaataa agacaagtac gcttccgtca
     6781 cttttgcagt aaacaaacta ttgtctggca aatcatcacc aaagatcgcc ttaatcgcca
     6841 gcaatgactg gacgcgcgct ttcccttcag cactactttg tacagccttc tgaataacag
     6901 gtaacagtgg gtcactgatt tcaatcggat ttccctgttc atcaacacca ccgacataac
     6961 gcatccaacc cgcgacgccc agtgccagca gatcgaactt gctgtcatgc gccagatgcc
     7021 agcgaacaga atccaacatc cgctgtggca atttctggct gccatccatc gcaatctgcc
     7081 aggttcgatg acgtaacgcc gggttgctat agcgttcaat taatcggtta gcgtaatctt
     7141 gcaaatcaac gccctgcact ttcagcgtcg gcgcttgttc ctgcaacatc aagccatacg
     7201 ccgcataacg ataatgttca tcttccatgc agtcattaat gtgctgatat ccagcaagat
     7261 aacccaaata cgccaggaat gaatgactgc cgttgagcat gcgcaacttc atctcttcat
     7321 aaggcagcac atcactaacc agttcggctc ccgctttttc ccattctgga cgtccggcaa
     7381 caaagttatc ttctattacc cactggcgga aaggttcaca ggcaacgccc gcaggatcgc
     7441 ccacaccggt aagttgttcg attttcgcca gcgtatcctc tgtcactgcg ggcacaatac
     7501 ggtccaccat tgttgatggg aaagtcacgt tatcttcgat ccattgtgcc agttttacat
     7561 caacagcttt cgcgtatgaa gtgacaacgt cacgcataac atgaccgttt tctggcatgt
     7621 tgtcacatga catgacggta aatgcgggaa gtcctgccgc tttacggcgg gccagcgcct
     7681 caacaatcac ccctgttgct gttttcggct ggtggggatt ttgcacgtcg gcagcgacca
     7741 tcgggtgatc gagcattaac tgtccggtcg ccggagagtg gaaataccct ttttcggtga
     7801 ttgtcagaga gacaatcgcg atttgcggtt cacacatcgc agccaacacg gtttctaagc
     7861 catccatctg tacgtgcaag gctttcttaa cgacgccaac gacgcgagcc gtccacgcat
     7921 cggccgacat ttccgcaacg gtataaagat tatcttgctg ttgtaaatcg gcaatttgct
     7981 gttcgccgcc gattaagttg acctcataat atccccagtc actgaaatgt tctgtagcaa
     8041 gaatatcggc atacacgccc tgatgcgcac ggtgaaatgc accaaagcct aaatgaataa
     8101 ttcttggggc caggttatta cgatcataaa cagggagtgt cgcttttgct gataacaaat
     8161 tatttcccat aacaattcct taaatataaa tatggcaagc tatatgtttt gtta
//
LOCUS       DAESHI010000162         8182 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.164, whole genome shotgun sequence.
ACCESSION   DAESHI010000162 DAESHI010000000
VERSION     DAESHI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.164"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..2847
                     /gene="yfaL"
                     /locus_tag="KW011_003914"
     CDS             <1..2847
                     /gene="yfaL"
                     /locus_tag="KW011_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HBH7662137.1"
                     /translation="ADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVIDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNP
                     NPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDG
                     QTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQ
                     GDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2975..3697)
                     /locus_tag="KW011_003915"
     CDS             complement(2975..3697)
                     /locus_tag="KW011_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HBH7662138.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3844..6471
                     /gene="gyrA"
                     /locus_tag="KW011_003916"
     CDS             3844..6471
                     /gene="gyrA"
                     /locus_tag="KW011_003916"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HBH7662139.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6679..>8182
                     /locus_tag="KW011_003917"
     CDS             6679..>8182
                     /locus_tag="KW011_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HBH7662140.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLTSLPKNLEPVFYNA"
BASE COUNT         1980 a         2031 c         2320 g         1851 t
ORIGIN      
        1 gccgacggaa aaacgctggt tattggcaat acagagaatg acggagctgt tgactctatt
       61 gctggtaccg ggttgatcac caaaacaggt tccggcgatc tggtacttaa tgcagataac
      121 aatgacttta ctggtgagat gcagattgaa aacggtgaag ttaccctggg ccgcagcaac
      181 tccctgatga atgtcggcga tacgcattgc caggacgatc cgcaagactg ctacggtctg
      241 acgataggga gtattgataa gtaccagaat caggcagagc taaacgttgg ctccacccaa
      301 caaacctttg tgcactcatt gacgggcttt cagaatggca ctttaaatat cgatgctggt
      361 ggcaatgtta ctgttaatca aggcagtttt gctggcacca tcgaaggtgc tggtcagctc
      421 accattgcgc aaaacggcag ctacgtgctg tcgggggcgc agtcgatggc gctaaccggc
      481 gatatagtcg ttgatgatgg tgcggtgctt tcgctggaag gcgacgcggc agatctcgcc
      541 gctctccagg acgatccgca gtcgatcgtg ttaaacggcg gtgtgctcga tctctctgat
      601 ttctccacct ggcagagcgg cacatcatac aatgatggcc ttgaagtcag tggcagcagc
      661 ggaacggtta tcggcagtca ggatgtggta gatcttgcag gtggcgacaa tttgcatatc
      721 ggcggcgacg ggaaagatgg cgtctacgtg gtggtcgatg cgagcgacgg gcaggtaagt
      781 ctggcaaaca ataatagtta tttgggcaca acacaaatcg cctccggtac gctgatggtg
      841 agcgacaact cgcagcttgg agatacccac tataaccgcc aggttatctt taccgataag
      901 caacaagaaa gcgtgatgga gattactgcc aatgtcgata ctcgctctac gacgactgag
      961 catgggcgtg atattgaaat gcgcgccgac ggtgaagtgg cagttgatgc gggggtagac
     1021 acgcagtggg gcgcactgat ggctgacagc agcgggcagc atcaggatga gggtagcaca
     1081 tttactaaaa cgggggcggg tacgctggag ctgaccgcca gcggtacaac gcagtcggca
     1141 gtacgtgtcg aagaaggcac cctgaaaggc gatgttgcgg atatctttcc ttatgcttcg
     1201 tcactgtggg tcggtgatgg ggcggcgttc gttactggcg cggatcagga tattcagtct
     1261 atcgatgcta cttccagcgg cactatcgat atcagcgatg gtacggtttt gcgtctgacc
     1321 gggcaggata cttccgtcgc ccttaatgcc tcattgttta acggcgatgg gacgctggtg
     1381 aatgctaccg atggcgtgac gttgacaggt gagcttaata ccaaccttga aactgacagc
     1441 ctgacctatc ttgccgacgt gacggttaat ggtgatctga ccaatacgtc cggtgcggtt
     1501 agcctgcaaa atggcgtcgc tggcgatacg ctgacggtaa acggtgatta taccggcggc
     1561 ggtacgctac tgctcgatag cgaattaaac ggcgatgact cggtaagcga tcaattggtg
     1621 atgaacggta atactgctgg caataccacg gtggtggtta actctattac agggattggt
     1681 gagccgacat cgacaggcat taaagtgatt gatttcgcag ctgatcccac gcagtttcaa
     1741 aacaatgcgc agttcagtct ggcaggcagc ggctacgtca atatgggagc gtatgactac
     1801 acgctggtgg aagataacaa cgactggtat ctgcgatcgc aagaagtaac gccgccatcg
     1861 ccacctaatc ccaacccgac tcccgatcct gatcccacac cggatcctga cccaaccccc
     1921 gacccggccc ctacgcctgc ttaccagccg gtgctgaatg ccaaagttgg cggttatctc
     1981 aataacctgc gggcggcaaa tcaggcgttt atgatggagc gacgcgatca cgctggtggc
     2041 gatggtcaga cgctgaattt acgtgttatc ggcggacgtt atcattacac ggcggcaggg
     2101 caactggctc agcaggaaga cacttctacg gtgcagctta gcggcggctt gtttagcggg
     2161 cgctggggca cggatggcga gtggatgctt ggggcggttg gtagctacag tgataatcag
     2221 ggcgacagcc gctcgaatat gaccggaact cgcgccgata accagaacca cggttatgcc
     2281 gttgggctga cctcaagctg gtatcagcac ggtaatcaga agcagggggc ctggctggat
     2341 aactggttgc agtacgcgtg gtttagcaat gatgtttctg aacatgaaga tggcacagat
     2401 cattaccatt cgtcggggat tatcgcctcg ctggaagccg ggtatcagtg gttaccgggg
     2461 cgtggtgtgg tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
     2521 tttaccgccg ctaaccatgc gcgcgtgtca caatcgcagg gggatgatat tcagacgcgg
     2581 ctgggtttac acagcgaatg gcgtaccgcg gttggtgtca caccaacatt agatctgaat
     2641 tattatcacg atccccatgc gacggaaatt gaagaagatg gcagcactat cagtgacgat
     2701 gcggtgaagc gacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtg
     2761 tccctgcgcg gcagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
     2821 tttttgtcga tgacggtgaa atggtaatcg ctgtctgacc atcacaattg ccggatgcgg
     2881 cgtaaacgcc ttatctggcc tacggagagc atttgtaggc ctgataagac gctgcaagcg
     2941 tcgcatcagg cattggcgcg gccaaacatc aacctcactt attctgcgtg tgcagcatat
     3001 agttcacatc cacgccgggg ccgagcttaa aggaattagt gatcgggttg taatgtagcc
     3061 cggttatatg tcgctctttt aaactggtct gatccaccca gccaagcagc tctgccggtt
     3121 taataaactt cttcacatca tgcgtacctt tgggcaccat gcgcaaaata tattccgcgc
     3181 caaccaccgc catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc
     3241 cgcctggttt caccagttgc gcacaggctc tgaccactga ctgcggatcg ggtacgtgct
     3301 ccagcatctc catgcaggtc accacatcat actgcccggc atgttttgcc gcgtgctctt
     3361 ccacggtttc ctgcacgtaa tccacctgaa tgccgctttc cagcgcgtgc agctttgcca
     3421 cctgcaatgg ctcaaagccc atatccagac cggtcaccgt cgcgccttcg cgcgccatac
     3481 tctcggccag aatgccgccg ccacaaccga catcgagcac ctttttgcca aataaaccgc
     3541 cagcacgctc ggcaatatag cccagacgca gcgggttaat gcggtgcaac ggtttgaact
     3601 caccttcaag atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt
     3661 ctacgttatg gttttccggc gatttttcgg cattcattgg cacttctact ccgtaattgg
     3721 caagacaaac gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaaac
     3781 tgcgcggctg tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt
     3841 tagatgagcg accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc
     3901 tcctatctgg attatgcgat gtcggtcatt gttggccgtg cgctgccgga tgtccgagat
     3961 ggcctgaagc cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg
     4021 aacaaagcct ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc
     4081 catggtgact cggcggttta tgacacgatc gtccgtatgg cgcagccatt ctcgctgcgt
     4141 tacatgctgg tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca
     4201 atgcgttata cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa
     4261 aaagagacgg tcgatttcgt tgataactat gacggcacgg aaaaaattcc ggacgtcatg
     4321 ccaaccaaaa ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca
     4381 accaacatcc cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat
     4441 gatgaagaca tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg
     4501 gcggcaatca ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgtggcaag
     4561 gtgtacatcc gcgcccgtgc tgaagtggaa gtcgacgcca aaaccgggcg tgaaaccatt
     4621 atcgtccacg aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa
     4681 ctggtaaaag aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac
     4741 ggtatgcgca tcgtgattga agttaaacgc gatgcggtcg gtgaagttgt gctcaacaac
     4801 ctctactccc agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat
     4861 ggtcagccga agatcatgaa cctgaaagat atcatcgcgg cgtttgttcg tcaccgccgt
     4921 gaagtggtga cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc
     4981 cttgaagcac tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat
     5041 gcgccgacgc ctgcagaagc gaaaactgcg ctggtggcta atccgtggca gctgggcaac
     5101 gttgccgcga tgctggaacg tgctggcgac gatgctgcgc gtccggaatg gctggagcca
     5161 gagttcggcg tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg
     5221 gatctgcgtt tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa
     5281 gagctgctgg atcagatcgc ggaactgttg cgtattcttg gtagcgccga tcgtctgatg
     5341 gaagtgatcc gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact
     5401 gaaatcaccg ccaacagcgc agacatcaac ctggaagatc tgatcaccca ggaagatgtg
     5461 gtcgtgacgc tctctcacca gggctatgtt aaatatcagc cgctttctga atacgaagcg
     5521 cagcgtcgtg gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac
     5581 cgactgctgg tggcaaacac gcacgaccat attctgtgct tctccagccg tggtcgcgtc
     5641 tattcgatga aagtttacca gttgccggag gccactcgtg gcgcgcgcgg tcgtccgatc
     5701 gtcaacctgc tgccgctgga gcaggacgaa cgtatcaccg cgatcctgcc agtgaccgag
     5761 tttgaagaag gcgtgaaagt cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc
     5821 ctcaccgaat tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc
     5881 gatgagctga tcggcgttga cctgaccagc ggcgaagacg aagtaatgct gttctccgcc
     5941 gaaggtaaag tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc
     6001 ggtgttcgcg gtattcgctt aggtgaaggc gataaagtcg tctctctgat cgtgcctcgt
     6061 ggcgatggcg caatcctcac cgcaacgcaa aacggttacg gtaaacgtac cgcagtggcg
     6121 gaatacccaa ccaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt
     6181 aacggtttag tggttggcgc ggtacaggta gatgactgcg accagatcat gatgatcacc
     6241 gatgccggta cgctggtacg tactcgcgtt tcagaaatca gcatcgtggg ccgtaacacc
     6301 cagggcgtga tcctcatccg tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt
     6361 gctgaaccgg ttgacgagga agatctcgat accatcgacg gcagtgccgc ggaaggggac
     6421 gatgaaatcg ctccggaagt ggacgttgac gacgagccag aagaagaata attttacttc
     6481 ttcatgccaa aagggagcta tctcccttgt ttgaattgaa aagtccaggc tgcaaagtct
     6541 gggcttttgt cgtattaggg cacggtaaag tttggctgtg cccgtaaaaa atggctggct
     6601 atacacaagg aatgtggcaa tgagtggtga aaaaaagcga aaggctggcg gttctatggt
     6661 cttgtaggtt ttggcgcaat agtactgctt tccgctggtg tctgggcgtt gcaatatgct
     6721 ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct gcaaatcgat
     6781 ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc caaagatctc
     6841 ctcaccattc cgtttctcca cgatgttctg agtgaagatt tcgttttcta ttatcagaat
     6901 catgccgatc gtctgggcat tgaaggcagc attcgtcgca ttgtctatga acacgatctc
     6961 acgctgaaag ataaactctt ttcgtcactc ttagatcagc ccgcgcaggc tgcgctatgg
     7021 cacgataaac aaggccatct ttcacattac atggtgctga tccagcgcag tggtttaagc
     7081 aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag caaaacggaa
     7141 atcagtagca tcaagataaa tagtgaaact attcctgttt atcagttgcg ctataacggc
     7201 aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc cagcacggat
     7261 atgttgttta aagatgatca gcaggatacc gaagccacgg cgatcgcagg tgatttgttg
     7321 agcggcaaaa aacgctggca agcaagcttt ggcctggaag agcgtgctgc tgaaaaaacg
     7381 ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg gttttggcta ccagcggtta
     7441 atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca cagttttgtg
     7501 gcgttaaatg atgaatccgc cagcgtagat accagtttcg attttacgcc ggtctggaac
     7561 agtatgcctg ccggggccag cttctgtgtg gtggtgccgt attcacacgg tattgccgaa
     7621 gagatgcttt cgcacatcag ccaggaaaac gacaagctga atggggcgtt agacggtgcc
     7681 gcggggctgt gctggtatga agactcaaaa ttgcaaaccc cgctgtttgt cggtcagttt
     7741 gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa tattggtgcg
     7801 cacgaaagca aagcgccaga aggtgttttg ccgataagcc agactcagca gggcgaagcg
     7861 caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc gcaggcggcg
     7921 caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat gcaaaacaaa
     7981 acgctgcttt tctccctcga tgacacgctg gttaataacg ccctgcaaac actgaataaa
     8041 aaccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct ctatatcaat
     8101 ccacaaggcg tggcgaaact gctgcgtaac gaaacgttga ccagtctgcc gaagaatctc
     8161 gaaccggttt tttataacgc cg
//
LOCUS       DAESHI010000163         8157 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.165, whole genome shotgun sequence.
ACCESSION   DAESHI010000163 DAESHI010000000
VERSION     DAESHI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.165"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..254
                     /locus_tag="KW011_003918"
     CDS             <1..254
                     /locus_tag="KW011_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HBH7662141.1"
                     /translation="RGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRY
                     FLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(303..1559)
                     /gene="nupG"
                     /locus_tag="KW011_003919"
     CDS             complement(303..1559)
                     /gene="nupG"
                     /locus_tag="KW011_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HBH7662142.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(1761..2840)
                     /gene="mltC"
                     /locus_tag="KW011_003920"
     CDS             complement(1761..2840)
                     /gene="mltC"
                     /locus_tag="KW011_003920"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HBH7662143.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(2905..3180)
                     /locus_tag="KW011_003921"
     CDS             complement(2905..3180)
                     /locus_tag="KW011_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HBH7662144.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(3208..4260)
                     /gene="mutY"
                     /locus_tag="KW011_003922"
     CDS             complement(3208..4260)
                     /gene="mutY"
                     /locus_tag="KW011_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBH7662145.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4421..5140
                     /gene="trmB"
                     /locus_tag="KW011_003923"
     CDS             4421..5140
                     /gene="trmB"
                     /locus_tag="KW011_003923"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBH7662146.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            5140..5466
                     /locus_tag="KW011_003924"
     CDS             5140..5466
                     /locus_tag="KW011_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HBH7662147.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            5650..6369
                     /locus_tag="KW011_003925"
     CDS             5650..6369
                     /locus_tag="KW011_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HBH7662148.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            6545..7591
                     /gene="ansB"
                     /locus_tag="KW011_003926"
     CDS             6545..7591
                     /gene="ansB"
                     /locus_tag="KW011_003926"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HBH7662149.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            7708..>8157
                     /locus_tag="KW011_003927"
     CDS             7708..>8157
                     /locus_tag="KW011_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HBH7662150.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNY"
BASE COUNT         2087 a         1988 c         2161 g         1921 t
ORIGIN      
        1 ttcgcggtga agtggagttg gtgcggattc gtgatgccga agggcgaatt gcggcagaag
       61 gggcgttgcc ttatccacct ggcgtgcttt gcgtggttcc cggggaagtc tggggtgggg
      121 cggttcaacg ttatttcctt gcgctggaag aaggcgtgaa tttgttgccg ggattttcgc
      181 cggagttgca aggtgtctat agcgaaaccg atgcggatgg catgaaacga ttgtacggtt
      241 atgtgttgaa gtaagaataa aaaaacgggt caccttctgg cgacccgttt ttctttgcgt
      301 aattagtggc taaccgtctg tgtgcctgtc gggacacgaa cgtgtttata tttgaacatc
      361 gccatgaacg cgaaggccag aaccacggaa taaccagcga aaatcaacca tacggtctgc
      421 cagtcggtaa tgccgttttg ggtgtacatc tcaacaactt taccgctcac gatgccgccg
      481 aggatacagc cgaagccgtt agtcatcatc aggaacatcc cttgtgcact ggcgcgaatt
      541 gccgggctaa cttctttttc gacaaacacc gaaccagaga tgttgaagaa gtcgaatgcg
      601 caaccgtaaa cgatcatcga cagtaccagc agtacagtac cgaacggagt cgggtcgccg
      661 taagcaaaca gcgcaaaacg caggatccac gccacaatac tgatcatcat tacgttctta
      721 ataccgtagc ggcttaagaa gaacgggatg gtcagaatga acagggtttc agagatctgc
      781 gaaatcgaca tgatgattga cgcatgctgc acgatgaagc tgctggcaaa catcggatct
      841 ttgtcgaagc tgtgcaggaa ggtattaccg aacatgttgg taatctgcag ttccgcgccc
      901 agcagcattg agaagatgaa gaagattgcc atacgcttgt ttttaaacag cgcgaatgca
      961 tcgaggccca gcagggttgt ccagctctga ttcgcttgct gtttagcaac cggaatatgc
     1021 ggcagagtca gggtaaacag aaccagaatg gcggaaagtg ctgcgccaat atacagctgc
     1081 atgtggctta attcgaagcc agacaggctc accacccaca ttgccatgat aaagccgatg
     1141 gtgccccaga tacggattgg cgggaagtca gtaacgatat ccatcccggc attttgcagg
     1201 cgatagtaag agatggtgtt gattaaccca agcgttggca tataagcaaa cgagttaatc
     1261 aatatcacaa ggaacatcgc ttccggcgtc gtgacctgtg ccgccatgaa cagcgtgata
     1321 gcgccaatgg tgtggcaaat ggcatatacc catttcgcac ttaaccattt gtcggccaca
     1381 atccccagca gcgcaggcat aaataccgcg gcgataccca gtgagctata aactgcgcca
     1441 atagaagcac cgtcaaactt cagggtaaca aacatatagg agccgagggt cgtcagccaa
     1501 cttccccaca gacagaactg cagaaaagag aggattttca gctgcagctt aagattcatg
     1561 ttaatttcct cacattgtga tgcggatgag tgtttgaaaa agcacatttg accgtgcgag
     1621 atgatgggat tctatcaact tcgcggactg gttttttgtt acctgtggca aataattgca
     1681 aacattccca atttgcaaaa cgaaattgtg atgtggataa catttttgcc cctgagcatc
     1741 gtcaggggcg gttaatggaa ttatcggcgg cggtaggatt tttgcgcggt attcacttta
     1801 taaagataac ggcgagattc cgcagaggga tggcgggtcg tcagcgtctg ataaacatcg
     1861 cccggcgtca tggtgttaat aatattggca gcctgaatct tatcattcga aaagactcgc
     1921 agcacgctgc ctgcgccgcc gttataggcg gtgatgacgg cataacgccg cgatgttggg
     1981 ttatcaattc cgccgagata aacattgttc agcatcgcca gatacgcggt gccggtatca
     2041 atattgctgg caggatcaaa caagaaactg cggctcggcg tgccggattt cccctgcgag
     2101 cggaacacat ctttcccggc agtatgttgt accacctgca ttaatcccag cgcatcggaa
     2161 cggctgaccg catacgggtt aaaggaagat tcggtctgca taattgccag aatcagcgac
     2221 tcatcaacgc catatttccg tgacgcctgg cggaccatgc cgagatattt gtgcgcacgt
     2281 ttatcaaggt ggttcggtac catgttaatg gtgacgctgt agatgatacg cagcccgtta
     2341 ctgcggcttt gcagacggtt tttcagcaga taatccgcga agttgcttgc gcgaccttcc
     2401 cagcgaatcg gctgcccggt gttgtccacc acctgaccgt aaaggaaagg ttctttcgaa
     2461 atcgtaatat catcaacgtc ggaatagaga tcgaccgaac tcggatcgtc acccatcagc
     2521 aacgttttga taattgcccg gcgcaaatgc gcggcaggtt ctgtcccggc gatagtttcg
     2581 atagtaatcg taccgtcatc gaagttgatg tggctgcggg tctgatattg atcggtgtat
     2641 ttcacgtagt ccttaggacc ggcgatcacc acctctttga atccccagat gttctcaata
     2701 ttgtgggcaa attgccccat cagaatatca aaaccgttgg tatctttgac ccaggcttcg
     2761 ttataggtat cgcctttttt ggtcgtcgaa caggagatga gcaacggcgc aatcaaagcc
     2821 agcgcgagat attttttcat cattccgggt gcgtgttgtg tttgttttta tgcaagttgc
     2881 cggaggcgtg ctccggcact gtttttattt tttatcttcc ggcgtatagc cctcgatatg
     2941 cacctcttta ccctcgaaca ggaagttgac catctcctgc tcaagcagct tgcggtgctc
     3001 ggcattcatc atgttgagtt tcttttcatt aatcagcatg gtttgcttgt gctgccactg
     3061 cgcccaggct tctttggaga tctcgttata gatgcgtttt cccagctcgc cggggtacag
     3121 ctgaaaatcc tgaccttctg cttcacgttg caggaaagta caaaaaatcg ttctgctcat
     3181 aaatcatcct ctttatcgac tcacgcgcta aaccggcgcg ccagtgcgta actgctgtaa
     3241 caaacgctcc acgggagccg ccaggccaac tgacggcggt tgcgctaagt tataccagag
     3301 cgcattgcct tcatccatgc agccggtgaa tgacgacacg ggaagccaca taggcacaat
     3361 atctaagtgg aaatggctga aggtatgccg aaacgcggtc agttgcgtca ggttatcggc
     3421 agcaatctgc cgttgcgcca gccactgccg caaactttct tcgtcggcaa actgcgggaa
     3481 acagtataaa ccgccccaca atccgctcgg cggacgctgc gccagcaata cttcatcttc
     3541 gtgctgtaac agcaaaaagt agccggtgcg ctccggcagc gtctgtttcg gttttttgcc
     3601 cggataaagc gaccagctat tattggcggc ggcaatacat ccgttttgta gcggacagag
     3661 cgaacatttc ggcttcgagc gcgtacaaat catcgcgccc aaatccatca tcgcctgatt
     3721 aaaccgttcc acgccgaccg cgggcgtcac ctgctcgctc aaactccata atttattctc
     3781 gacctctttt ttcccaggcc agccgcttac agcatagcag cgcgccagca cgcgtttgac
     3841 gttaccgtcg agaatcggaa agtgcttacc cagagaaagc gagagaatcg cgcctgcggt
     3901 ggaacgcccg acgcccggta acgccgcgac ttcttcaaag gtttccggga atttaccgcc
     3961 gtgtaaggtc gccacttgtt gtgccgcttt atgcagattg cgcgcgcggg cgtaatagcc
     4021 aagcccggtc cacaagtgga gaacttcgtc gagcggcgca ttggcgagat cggtcaccgt
     4081 cgggaagcgc gccataaagc gttcaaaata ggggataacg gtcgcaacct gagtttgttg
     4141 caacatcact tctgagagcc atactttgta gggcgtcttg tcaatttgcc agggcagcgt
     4201 ttttcgcccg tatttatcgt accagtccag aacctgggct gaaaattgcg acgcttgcat
     4261 ggtcaccgaa ttcactgttg ttgggggcaa gattgcagca cagcgacggc agggtgtaaa
     4321 ccggaacttt ccgcagccac ggccttatca tgcttgcatc cggcaactaa ctttggataa
     4381 tgcccgtttt cagaacactt tcacaagcga ctaaatcttt atgaaaaacg acgtcatttc
     4441 accggaattt gatgaaaacg gccgcccact gcgccgtatc cgtagttttg tgcgccgcca
     4501 ggggcgactg accaaaggcc aggaacatgc gctggaaaac tactggccgg tgatgggcgt
     4561 tgagttcagc gaagatatgc tggatttccc cgcgcttttt ggccgtgaag cgccggtgac
     4621 gcttgagatt ggttttggca tgggggcgtc gctggtggca atggctaaag atcgccctga
     4681 gcaggacttc ctcggcattg aagtgcattc accgggcgtt ggtgcgtgcc tggcttctgc
     4741 gcatgaagaa ggtttaagca acctgcgcgt gatgtgtcac gatgcggttg aagtgctgca
     4801 taaaatgatt cctgacaatt cattgcgcat ggtgcagctc tttttccctg acccgtggca
     4861 caaagcgcgc cataataaac gccgtatcgt acaggtgccg tttgccgaac tggtaaaaag
     4921 caaactgcag ctggggggtg tattccatat ggcgaccgac tgggaacctt atgcggaaca
     4981 tatgcttgaa gtgatgtctt ctattgacgg ttataaaaac ctgtcagaga gcaatgatta
     5041 cgtgccgcgt ccggcatcac gtccggtgac gaaatttgaa caacgtggtc atcgtcttgg
     5101 tcacggagta tgggacttaa tgttcgagag ggtgaaataa tggcaaagaa ccgtagccgt
     5161 cgtctgcgta aaaaaatgca catcgacgaa ttccaggaat taggattttc ggtggcatgg
     5221 cgattcccgg aaggtacatc ggaagaacag attgataaaa ccgttgatga ttttattaac
     5281 gaggttatcg aaccgaacaa actggccttt gacggcagcg gttatctggc ctgggaaggt
     5341 ctgatctgca tgcaggaaat cggcaaatgc accgaagaac atcaggcgat tgtgcgtaag
     5401 tggctggaag agcgcaaact ggatgaggta cgcaccagcg aacttttcga cgtttggtgg
     5461 gactaagaaa gcatacgggc gatgacaaat gcaaaactgc ctgatgcgct acgcttatca
     5521 ggcctggaaa gatgcacgat cgagtaggcg ggataaggtg tttacgccgc atccggcatg
     5581 gaaaacgcgt actttgttat caatctgggg ccagcaaatg ctggcctgat tttttcttga
     5641 gggaagacta tgatgcgcaa aatgctgctg gcggcagcac tttcagtgac ggcaatgacc
     5701 gctcacgccg actaccagtg cagcgtcacg ccgcgtgacg atgtgattgt cagcccgcaa
     5761 accgtgcagg tgaagggcga aaacggcaat ctggtgatca cgccagacgg caacgtgatg
     5821 tataacggta agcaatattc cctgaatgcc gcccagcgcg agcaggcgaa ggattatcag
     5881 gctgaactac gcagcacgct gccgtggatt gatgaaggcg cgaaaagccg cgtcgaaaaa
     5941 gcccgtattg cgctggataa aattatcgtt caggagatgg gcgaaagcag caaaatgcgc
     6001 agccgtctga ccaaacttga tgcgcagctg aaagagcaga tgaaccgcat tatcgaaacg
     6061 cgcagcgatg gcctgacgtt tcactataaa gccattgatc aggttcgcgc cgaaggccag
     6121 caattagtga atcaggcaat gggtggaatt ttacaggaca gcattaatga aatgggcgcg
     6181 aaagcggtgc tgaaaagcgg cggtaaccca ttacagaatg tgctgggaag cctggggggg
     6241 ctgcaatcct caatccaaac agagtggaaa aagcaggaaa aagatttcca gcagtttggc
     6301 aaagatgttt gtagccgcgt tgtgactctg gaagatagcc gcaaagccct ggtcgggaat
     6361 ttaaaataat cctctatttt aagacggcat aatacttttt tatgccgttt aattcttcgt
     6421 tttgttacct gcctctaact ttgtagatct ccaaaatata ttcacgttgt aaattgttta
     6481 acgtcaaatt tcccatacag agctaaggga taatgcgtag cgttcacgta actggaggaa
     6541 tgaaatggag tttttcaaaa agacggcact tgccgcactg gttatgggtt ttagtggtgc
     6601 agcattggca ttacccaata tcaccatttt agcaaccggc gggaccattg ccggtggtgg
     6661 tgactccgca accaaatcta actacacagc gggtaaagtt ggcgtagaaa atctggttaa
     6721 tgcggtgccg caactaaaag acattgcgaa cgttaaaggc gagcaggtag tgaatatcgg
     6781 ctcccaggac atgaacgata atgtctggct gacactggcg aaaaaaatta acaccgactg
     6841 cgataaaacc gacggcttcg tcattaccca cggtaccgac acgatggaag aaaccgctta
     6901 cttcctcgac ctgacggtga aatgcgacaa accggtggtg atggtcggcg caatgcgccc
     6961 gtccacgtcc atgagcgcag acggtccatt caacctgtat aacgcggtag tgaccgcagc
     7021 tgataaagcc tccgctaatc gtggcgtgct ggtggtgatg aacgacaccg tactggacgg
     7081 tcgcgatgtc accaaaacca acaccaccga cgtagcgacc ttcaagtctg ttaactacgg
     7141 tcctctggga tacattcaca acggtaagat tgactaccaa cgtaccccgg cacgtaagca
     7201 caccagcgat acgccattcg atgtctctaa gctgaatgag ctgccgaaag tcggcatcgt
     7261 ttataactac gctaacgcat ccgatcttcc ggctaaagca ctggtagatg cgggctatga
     7321 tggcatcgtt agcgctggtg tgggtaatgg taacctgtat aaatccgtgt tcgacaccct
     7381 ggcaaccgcc gcgaaaaacg gcactgcagt agtgcgttct tcccgcgtac cgacgggtgc
     7441 taccactcag gatgctgaag tggatgatgc gaaatacggc ttcgtcgcct ctggcacgct
     7501 gaacccgcaa aaagcgcgcg tcctgctgca gctggctctg acgcaaacca aagatccgca
     7561 gcagatccag cagatcttca atcagtacta atcacctcgc cccggtatcg tgccggggct
     7621 ttttcacttc agacttacgt ccattgccaa ttttcattac cctaatgata atcaccggaa
     7681 taaattattc cgcgtgaggt ttttcgggtg aaaaagcaat ggattgttgg cacggcactg
     7741 cttatgttga tgactggtaa tgcctgggca gatggcgaac cgccaactga aaatatctta
     7801 aaagatcaat tcaaaaagca gtatcacggc attctcaagc ttgatgctat caccttaaaa
     7861 aatcttgatg ctaagggtaa tcaggccacc tggtcagcgg aaggcgatgt ctcttccagt
     7921 gacgatctct atacctgggt cggtcagttg gcagattacg aactgctcga acagacctgg
     7981 acgaaagata aaccggtaaa attctcggcg atgttaacca gtaaaggaac gcccgcgtct
     8041 ggctggtcgg tgaactttta ctcttttcag gcggcagcca gcgatcgtgg gcgggtggtc
     8101 gacgatatca aaacgaataa taaatatctg atcgtgaata gcgaagattt caattat
//
LOCUS       DAESHI010000164         8054 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.166, whole genome shotgun sequence.
ACCESSION   DAESHI010000164 DAESHI010000000
VERSION     DAESHI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8054
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.166"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            103..468
                     /locus_tag="KW011_003928"
                     /pseudo
     CDS             103..468
                     /locus_tag="KW011_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
     gene            complement(525..2903)
                     /gene="ppsA"
                     /locus_tag="KW011_003929"
     CDS             complement(525..2903)
                     /gene="ppsA"
                     /locus_tag="KW011_003929"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HBH7662151.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            3236..4069
                     /gene="ppsR"
                     /locus_tag="KW011_003930"
     CDS             3236..4069
                     /gene="ppsR"
                     /locus_tag="KW011_003930"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HBH7662152.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            4226..5272
                     /gene="aroH"
                     /locus_tag="KW011_003931"
     CDS             4226..5272
                     /gene="aroH"
                     /locus_tag="KW011_003931"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HBH7662153.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            5404..5595
                     /gene="hemP"
                     /locus_tag="KW011_003932"
     CDS             5404..5595
                     /gene="hemP"
                     /locus_tag="KW011_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HBH7662154.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(5599..7035)
                     /locus_tag="KW011_003933"
     CDS             complement(5599..7035)
                     /locus_tag="KW011_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HBH7662155.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(7098..7811)
                     /gene="ydiV"
                     /locus_tag="KW011_003934"
     CDS             complement(7098..7811)
                     /gene="ydiV"
                     /locus_tag="KW011_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HBH7662156.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT         2073 a         2112 c         1910 g         1959 t
ORIGIN      
        1 ccgtcagtag ctgaacagga gggacagctg atagaaacag aagccactgg agcacctcaa
       61 aaacaccatc atacactaaa tcagtaagtt ggcagcatca ccaaaattac cggacgcaaa
      121 aaagatatta ttgtccgcgg cggcgaaaat attagcagcc gtgaagtaga agatatttta
      181 ttgcagcatc ctaaaattca cgatgcctgt gtggttgcaa tgcccgatga acgtttaggt
      241 gaacgatcat gcgcttatgt cgtgctgaaa gcgccgcatc attcattatc gctggaagag
      301 gtggtggctt tttttagccg taaacgggtc gcaaaatata aatatcctga acatatcgtg
      361 gtaatcgaaa aactaccgcg tactgcctca ggtaaaatac aaaagttttt gttaagaaaa
      421 gatattatgc ggcggttaac tcaggatgtc tgtgaagaga ttgaataagt ttcatcttcg
      481 gggatcacat aaccccggcg actgaacgcc gccggggatt tattttattt cttcagttca
      541 gccaagctta accaggtttg caccacggtg tccgggttca gagacaggct atcgatcccc
      601 tcttccatca accatgcggc aaagtcttcg tggtcggacg gaccctgacc gcaaatcccg
      661 acatatttgc cctgtttctt cgcggcacgg atagccatcg acagcagtgc tttcaccgca
      721 tcgttgcgct catcgaacaa ttcagacacc acgccggagt cacggtccag accgagcgcc
      781 agctgcgtca tatcgtttga gccaattgag aagccgtcga aatattcgag gaactgctcg
      841 gccagcaagg cgttggacgg gatttcacac atcatgatga ttttcagccc gttctcacca
      901 cgctttagcc cctgacgcgc cagttcctca accaccgctt tcgcctgatc tacggtacgc
      961 acgaacggga tcatgatctc aacgttggtc agccccatgt cgttgcgcac acgtttcact
     1021 gcttcgcact ccagcgcgaa gcagtcgcgg aagctgtcgg aaacatagcg tcccgcgcca
     1081 cggaagccga gcatcgggtt ctcttcatct ggctcgtaac gctcaccacc gaccaggttg
     1141 gcatattcgt tcgatttaaa atcagagaga cggacaatga cgcgcttcgg ataaaacgcg
     1201 gcacccagcg tcgcgatccc ttcagtcaga cgaccaacgt aaaattcacg cggagaatca
     1261 aaacctttca tcatctcgcg gatttcattt tgcaactgcg gttcctgatc gtcaaactca
     1321 agcagtgcgc gtgggtggac gccaatcata cggttgatga taaattccag acgcgcaagg
     1381 cccacgcctt cgttcggcag gcaggcgaag tcgaaagcac ggtccgggtt accgacgttc
     1441 atcatcactt tcaacggcag atccggcatc atttctacgc tggagctttt cacgctaaat
     1501 tccagcaact ccgcatagac gtaaccggta tcaccttcgg cacaagaaac agtgacgttc
     1561 tcaccgtctt tcatccgttc tgttgcatca ccacagccca ctaccgccgg aatgcccagt
     1621 tcacgagcga tgatcgccgc gtgacaggta cgaccgccac ggttggtgac gatggcagat
     1681 gctttcttca tgatcggttc ccagtccggg tcggtcatgt cagtgaccag cacgtcacca
     1741 ggttcgatgc ggttcatttc gctgatctca tggatgactt tcaccggacc cgcgccgatg
     1801 cgatgaccga tagcacggcc ttcggcgata atcttaccct gtgaatgcag cgtataacgc
     1861 tccatgacct gaccgcgtga gcgcacggtt tccggacgcg cctgcacaat gaacagtttg
     1921 ccagtgtggc catctttcgc ccactcaata tccatcgggc gaccgtagtg tttctcaatt
     1981 tgtacggcct gttttgccag ttcctgcact tcttcgttgg tcagcgagaa gatgtcacgc
     2041 tgttcctgcg gtacgtcttc gattttaacc tgcttgccgt gctcctgggt cggcgcgtaa
     2101 accatgcgga tttttttcga ccccatggtg cgacgcacga tagccgggcg attcgccgcc
     2161 agtgtcggtt tatggacgta aaactcatcc gggttaaccg caccctgcac gaccatttcc
     2221 ccaaggcccc atgcggaagt gataaacacc acctggtcaa agccggattc ggtatcaatg
     2281 gagaacatca cgccagaaga tgcgaggtcg gagcgcacca tccgctgaac accggcagag
     2341 agcgccacgc cacggtgatc gtaaccctgg tgcacacgat aagagatggc gcgatcgtta
     2401 aacagagaag caaatacatg cttcactgcc acgagaacgg cgtcaaaacc ctgaacgttg
     2461 aggaaggttt cctgctgacc ggcaaaagaa gcgtccggca tatcttctgc ggtggcggag
     2521 gagcgcaccg caaaagaggc gttttcgtca tcggcggaaa gctgtgcata ggcttcgcgg
     2581 atggcgtttt ccagctcagg ctggaaggga gtgtcgataa tccactggcg gatttgcgcg
     2641 cccgctttcg caagctgagt aacatcgtca atatccgttt tatccagcag ttcataaatg
     2701 cgctggttta cgccgctttg gtccagaaac tggttaaacg cgtcggcggt tgtggcgaaa
     2761 ccattcggaa cggaaacacc cattccggaa aggttagtaa tcatttcacc cagggaggca
     2821 tttttgcccc caaccctgtc tacatcattc atgccgagtt ggttatacca aagcaccagc
     2881 ggtgacgagc cattgttgga catcgaacaa tccttttgtg ataaatgaac ggtttgagaa
     2941 acacatttct gcgcatttat ctttgcatat ttaaccggat gaaaaaaacg gtgaatcgtt
     3001 caagcaaata tattttttta ctttttaaga ctgatcccag cgttgcgcaa atctgctctc
     3061 ttcgacaatt tccacataaa ccatcggtat aaacgaaacc ataaaaatga aatgctgttt
     3121 tcataaaaaa taaaattgaa ggttcatttt ataaaccaga cataacgtcc tacgcttctg
     3181 tgcgttttta atttatgctt tcatagaatt atgtctgcat cacgggaaga acaaaatgga
     3241 taatgctgtt gatcgccacg ttttttatat ttctgatggt acggcaataa ctgcggaggt
     3301 attaggacac gcagtaatgt cacaatttcc cgtcactatc agcagcatta cgctgccgtt
     3361 tgtcgaaaat gagagccgtg cacgggcagt gaaggatcag attgacgcaa tttatcacca
     3421 gacaggtgtg cgcccgctgg tcttctactc catcgtgttg ccggagattc gcgccatcat
     3481 cttgcaaagt gaaggctttt gccaggatat cgttcaggcg ctggttgccc cgctacaaca
     3541 agagatgaaa ctggatccaa cgccgattgc tcatcgtacc catggcctta accctaataa
     3601 tctcaataaa tatgatgcgc gcattgcggc gattgattac accctcgccc acgatgacgg
     3661 catttcgttg cgcaatctgg atcaggctca ggtgatcctg ctcggtgttt ctcgctgtgg
     3721 taaaaccccc accagtcttt atctggcaat gcaatttggt atccgcgcgg caaactaccc
     3781 ctttattgcc gacgatatgg ataatctggt gctacccgcg tcgctcaaac cgcttcagca
     3841 taaattgttc ggcctgacta tcgacccgga acgtctggcg gcgattcgcg aggaacgtcg
     3901 ggagaacagt cgctatgcct cgcttcgtca gtgcaggatg gaagtcgcgg aagtggaagc
     3961 gttgtaccgt aaaaatcaga tcccgtggat taacagtacc aattattcgg tagaagagat
     4021 tgccaccaag atcctcgata tcatgggcct tagtcgccga atgtactaga gaactagtgc
     4081 attagcttat ttttttgtta tgatgctaac cacccggcga ggtgtgacac acctcgcact
     4141 tgaaatcagc agcgattggt ttatcgtgat gcgcatcact tcccggcagt cctgccgtag
     4201 aagcaacaaa tttctgagac ttgtaatgaa cagaactgac gaactccgta ctgcgcgtat
     4261 tgagagcctg gtaacgcccg ccgaactcgc gctacggtat cccgtaacgc ctggcgccgc
     4321 cacccatgtc accgactccc gccgcagaat tgaaaaaata ctcaatggtg aagataagcg
     4381 actgttggtc attattggcc cctgctcgat ccacgatctc accgctgcaa tggagtacgc
     4441 cccccgtctg cagtcgctgc gcaaccagta ccagtcacgg ctggaaatcg taatgcgcac
     4501 ctattttgaa aaaccacgaa ctgttgtcgg ctggaaagga ctaatctccg atccagattt
     4561 aaacggcagc tatcgggtaa atcacggtct ggagctggcg cgcaaattac ttttacaggt
     4621 aaatgagctg ggcgtcccaa ccgcgactga gttcctcgat atggtgaccg gtcagtttat
     4681 tgctgattta atcagttggg gcgcgattgg cgcacgtact accgaaagtc agatccaccg
     4741 tgaaatggct tcggcactct cctgtccggt aggttttaaa aatggtaccg atggcaatac
     4801 gcggattgct gtggatgcta tccgcgcagc ccgcgccagc catatgttcc tctcgccaga
     4861 caaaaatggt cagatgacca tctatcagac cagcggcaac ccgtatggcc acattattat
     4921 gcgtggcggc aaaaaaccga attatcatgc cgatgatatc gccgcagcct gcgatacgct
     4981 gcacgagttt gatttacctg aacatctggt ggtggatttc agccacggta actgccagaa
     5041 gcagcaccgt cgccagttag aagtttgtga ggatatttgt cagcaaatcc gcaatggctc
     5101 tacggcgatt gctggaatta tggcggaaag tttcctgctc gaaggaacgc aaaaaatcgt
     5161 cggcagtcag ccgctcactt acggtcaatc cattaccgac ccgtgtctgg gctgggaaga
     5221 taccgaacgc ctggtcgaaa aactcgcctc tgcggtagat acccgcttct gaatgcgtgc
     5281 ccattcctga cggaatgggc atttctgcgc aacttgttgt cttctcaaca aattactgct
     5341 tgctctggcc tgccataata ttgataataa gaatcattgt tatatcaatt attattaatc
     5401 tttatgcgtt atacggatag cagaaaactc acgcctgaaa cggacgccaa tcacaaggcc
     5461 gcttccccgc agcccatccg gcgaattacc agccagacac tgttaggtcc ggatggcaaa
     5521 ctgattatcg atcatgacgg gcaagaatat ctgctccgta aaactcaggc tggcaagctg
     5581 ctgttaacta agtagccttt aactcgagca gctgacttcc agccgtttac cccagtcagg
     5641 tggacgactg acatagtcat catctctatc gctgaaagga tttcgcaacg ccccatgcag
     5701 gcggtgcaat tccgtcatat cacccttttc tgccgcttca atcgcccgtt gcgccaacca
     5761 gttgcgcaat accagagcgg ggttaacgct ttgcatcaac tgctgacgct cactatcgct
     5821 aacctcgtct tgttgcaaac gcccccgata acgggcaaac cagccatcaa atgccgcacg
     5881 atcaataaac tcatcacgta gcggtgacgc cgtgctgtgc tgctcggtca gactcagcat
     5941 gcggaatgtg cgggtataat cgctgcgctc tcgcgccatc agactgaata attcattcag
     6001 tagcgcgtta tcctcttttt gctccgtgat gaagcccagt ttctgccgca tccgttgtcc
     6061 ataatgcgtc aacaaaacct gctgatagct gtccagggcc tcattcaggg catctacggc
     6121 aacaaatggt gacaatgtct gcgccagacg ctgtaaattc cacaacgcga cagcaggttg
     6181 attatcaaaa ctataacgcc cttgatgatc cgagtgatta caaataaaac cgggttcgta
     6241 atcatcaaga aaaccaaacg gcccgtaatc aagcgtcagt cccagcagcg acatgttgtc
     6301 ggtattcatc accccatgtg caaagccgac cgtctgccat tgggcaatta acgacgcggt
     6361 acgtgcgaca acatcgctaa accagagacg gtatttgtcc tcatcatctt caagatgtga
     6421 ccagtaatga cgaatagcaa agtcagctaa ctgacgaacc ttttccgact cgcggcggta
     6481 gtaaaaatgt tcgaaatgac caaagcgcag atgacttggt gccacacgca tcagcatcgc
     6541 gcctggctcc gccgtttccc gatacactgg cgaatcgctg gtgacgatac ttaacgcgcg
     6601 ggtcgtcgga atgcccagat aatgcatcgc ctcactggcg agactttctc gtatcgtcga
     6661 acgtaaaacc gcccgtccat cacccattcg cgaataaggc gtcaggccag cacctttcag
     6721 atgccagtcc attgtagtgc catcagcaag cagttgttcg ccgagtaaaa tgccgcgccc
     6781 atcacccagt tggcccgccc agacgccgaa ctgatgacca ctgtaaacct gcgccagtgg
     6841 tgacatacca ggcagtaggg cttcgccgcc ccagacacct gcgccatttt taaacagcga
     6901 cgatggaata ctcagcgtgt tagccagttc ggtattatgc caaatcagcc gggcattatt
     6961 taaaggcgta ggggaaagtg ctgtataggt ttctggcaat tcatcgcgcc agcgggtaac
     7021 aaaagacagg gtcatagatc ctcctgtttg atagtgtaga cggttactct cgtcttaaac
     7081 accagcaaac agaagggtta tcgctgaacc aacgtcgtta tctgatttat gggaacggca
     7141 ggccacaggc atccctggag agcatggaaa tcgaaggggg taatctgtgc caatatttct
     7201 gccgtatcaa tgcctcccgc aatgatgcaa ttgcaacaag gggaaatttg cgcctggatc
     7261 gcacgaataa acggttcgaa ggagcggtgc gtgatttgtt gctgaataaa acttttatcc
     7321 agcatgacgc gggtgaaaag accatcaaaa accgctttca ttgtgctatt gcctgctccc
     7381 aaattaccta acacaagggg atacacttgc gataaggata aaagaccgcg attatctttt
     7441 ccttcattga gatgcgggta attctcatta atcaacagtt caataaacgg atattttaat
     7501 agttcgcctg cgtaattatc cctctctaat aataatgtag ccacttgtgg cgttaaatta
     7561 agccaggcaa agagtttatg ttgaataaaa aaatgttggc atgattttag taattccaac
     7621 tgctctgaaa ataattgcca atgttgctcc tcggtaagtt gcgctataac ccgactcgtc
     7681 gggatacgca ccgtgccatc ttcactggag aaatgggtaa ttaactcaac gccgacaagg
     7741 acttgctggt tatctcttat cggcaggaaa tagcaatcag aatgataaag attctccaaa
     7801 aaaatcttca tggtaaccgt ccctctcgaa ggatgttttt cagtatccgg ctaaaaggga
     7861 tgaggccata agacataaca attacagaag gagtaacttt catttgttcc atgttaacca
     7921 ctttttcagg ggtccttttt ttagattatc ctgattataa acgaataatc catttacgga
     7981 atttttgtct gcaaaatact actgtattca ggagtaaatg gacggcgatg tatcatcgcc
     8041 gtccattttt cgac
//
LOCUS       DAESHI010000165         7799 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.167, whole genome shotgun sequence.
ACCESSION   DAESHI010000165 DAESHI010000000
VERSION     DAESHI010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.167"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..644
                     /locus_tag="KW011_003935"
     CDS             <1..644
                     /locus_tag="KW011_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH7662157.1"
                     /translation="HAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICLS
                     LADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASGM
                     RGELKLFQRFNMLIESHFHQHWTVPDYANELHITESRLTDICRRFANRPPKRLIFDRQ
                     LREAKRLLLFSDNAVNNIAWQLGFKDPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            895..2457
                     /gene="hpaB"
                     /locus_tag="KW011_003936"
     CDS             895..2457
                     /gene="hpaB"
                     /locus_tag="KW011_003936"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HBH7662158.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2475..2987
                     /locus_tag="KW011_003937"
     CDS             2475..2987
                     /locus_tag="KW011_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HBH7662159.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3380..5530
                     /gene="btsT"
                     /locus_tag="KW011_003938"
     CDS             3380..5530
                     /gene="btsT"
                     /locus_tag="KW011_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HBH7662160.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5580..5783
                     /locus_tag="KW011_003939"
     CDS             5580..5783
                     /locus_tag="KW011_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBH7662161.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5794..6750
                     /gene="yjiA"
                     /locus_tag="KW011_003940"
     CDS             5794..6750
                     /gene="yjiA"
                     /locus_tag="KW011_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HBH7662162.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6828..7724)
                     /locus_tag="KW011_003941"
                     /pseudo
     CDS             complement(6828..7724)
                     /locus_tag="KW011_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
BASE COUNT         1797 a         2133 c         2039 g         1830 t
ORIGIN      
        1 ctcatgcgtt tattacggag tctgacgccg acggtcatgt gttgacggta cgggaagatc
       61 tgatctggcc cctgctggaa gttctttatc caggcactcg ggaaaccttc ggcctgccgg
      121 ggatttgcct gtcactggca gataaacccg acgaactggc ggcgctggaa cactattggc
      181 aactgataga gcgggaatcg gtggaacaac tgcctggacg ggaacacacc ctgacgctac
      241 tggcacaggc agtgttcacc ctactgctgc gtaatgcaaa actcgacgac catgccgcca
      301 gcgggatgcg cggagaatta aaactgttcc agcgttttaa tatgctgatt gaaagccatt
      361 ttcatcagca ctggacagta ccggattacg ctaacgaact gcatatcacc gaatcacgcc
      421 tcacggacat ctgccgccgc tttgccaacc gtccgccaaa acggttgatt ttcgacaggc
      481 agctgcgaga agccaagcgg ctgctgctgt tttctgataa cgccgtgaac aatattgcct
      541 ggcaactcgg ttttaaggat ccagcttatt ttgcgcgctt ttttaatcgc ttagtcggtt
      601 gctcgcccag tgcttatcgt gccaaaaaag tacctgtgac gtgaaaatcc cttagcctta
      661 acgggaaacc aggcaccacc tgctattccc ctttctccgc ctggagcaag gggatttagt
      721 aacgtcaaaa atcgctaaaa gcgaccgcga tcacaaaacc gtacccgacc tgaaaagtac
      781 cagccattgt cccaaaagtg tcttcccgta atcgcccctg caagcttcaa ttgaataaca
      841 aaaacaacac acaaaattaa caatcgtatt ccgattaata ctgtagaggt cgacatgaaa
      901 ccagaagatt tccgcgccag tacccaacgt ccgttcaccg gggaagagta tctgaaaagc
      961 ctgcaggatg gtcgcgagat ctatatctat ggcgagcgag tgaaagacgt cactactcat
     1021 ccggcatttc gtaatgcggc agcgtctgtt gcccaactgt acgacgcgct gcacaaaccg
     1081 gagatgcagg actctctgtg ctggaacacc gacaccggca gcggcggcta tacccataaa
     1141 ttcttccgcg tggcgaaaag tgccgacgac ctgcgccagc aacgtgacgc catcgctgag
     1201 tggtcacgcc tgagctatgg ctggatgggc cgtaccccag actacaaagc tgctttcggt
     1261 tgcgcactgg gcgcgaatcc gggcttttac ggtcagttcg agcagaacgc ccgtaactgg
     1321 tacacccgta ttcaggaaac tggcctctac tttaaccacg cgattgttaa cccaccgatc
     1381 gatcgtcatt tgccgaccga taaagtgaaa gacgtttaca tcaagctgga aaaagagact
     1441 gacgccggga ttatcgttag cggtgcgaaa gtggttgcca ccaactcggc gctgactcac
     1501 tacaacatga ttggcttcgg ctcggcacaa gtaatgggcg aaaacccgga cttcgcactg
     1561 atgttcgttg cgccaatgga tgccaatggc gtcaaattaa tctcccgcgc ctcttatgag
     1621 atggtcgcgg gtgctaccgg ctcgccgtac gactacccgc tctccagccg cttcgatgag
     1681 aacgatgcga ttctggtgat ggataacgtg ctgatcccat gggaaaacgt actgatctac
     1741 cgcgatttcg atcgctgccg tcgctggaca atggaaggcg gttttgcccg tatgtatccg
     1801 ctgcaagcct gtgtgcgcct ggcagtgaaa ctcgacttca ttacagcact gctgaaaaaa
     1861 tcactcgaat gtaccggcac cctggagttc cgtggtgtac aggccgatct cggtgaagtg
     1921 gtggcgtggc gcaacacctt ctgggcattg agtgactcga tgtgttctga agcgacgccg
     1981 tgggtcaacg gggcttattt accggatcat gccgcactgc aaacctatcg cgtactggca
     2041 ccaatggcct acgcgaagat caaaaacatt atcgaacgca acgttaccag tggcctgatc
     2101 tatctccctt ccagtgcccg tgacctgaac aatccgcaga tcgaccagta tctggcgaag
     2161 tatgtgcgcg gttcgaatgg tatggatcac gtccagcgca tcaagatcct caaactgatg
     2221 tgggacgcca ttggcagcga gtttggtggt cgccacgaac tgtatgaaat caactactcc
     2281 ggtagccagg atgagattcg cctacagtgt ctgcgccagg cacaaagctc cggcaatatg
     2341 gacaagatga tggcgatggt tgatcgctgc ctgtcggaat acgaccagaa cggctggact
     2401 gtgccgcact tgcacaacaa cgacgatatc aacatgctgg ataagctgct gaaataacgc
     2461 agcaggaggt taagatgcaa ttagatgaac agcgcctgcg ctttcgtgac gcgatggcca
     2521 gcctgtcggc agcggtaaat attatcacca ccgagggcga cgccggacaa tgcgggatta
     2581 cggcaacggc cgtctgctcg gtcacggata caccaccatc gctgatggtg tgcattaacg
     2641 ccaacagtgc gatgaacccg gtttttcagg gcaacggtaa gttgtgcgtc aacgtcctca
     2701 accatgagca ggaactgatg gcacgccact tcgcgggcat gacaggcatg gcgatggaag
     2761 agcgttttag cctctcatgc tggcaaaaag gtccgctggc gcagccggtg ctgaaaggtt
     2821 cgctggccag tcttgaaggt gagatccgcg atgtgcaggc aattggcaca catctggtgt
     2881 atctggtgga gattaaaaac atcatcctca gtgcagaagg tcacggactt atctacttta
     2941 aacgccgttt ccatccggtg atgctggaaa tggaagctgc gatttaagct ccagcccctt
     3001 cacgcattcg tcgaaggggc atttttattg tgatctcaat cacatatcca cccgatgaat
     3061 aattcttgcc caaccgctca ccgccattcc tgaccgctta cgtccctaaa ataaccactc
     3121 agttatttac cttactttac gcgcgcgtaa ctctggcaac atcactgcag gatagcggcc
     3181 aatttacctc ctcaaacgca acgcaaacct agaacggctt cggcctatga ttaacaaatt
     3241 actctatgaa ttttggtttg caggaaggca gcaagtgagt gaatccccgg gagcttacaa
     3301 cagtaagtga caggggtgaa cgaacgcaac tgccgcacct gtaagccaaa agacgacgag
     3361 taaaatgcca ggttttacta tggatacgaa aaaactattc aagcacatac cctgggtgat
     3421 tctcggaatc atcggtgcat tctgcctcgc ggtagttgca ttacgtcggg gggagcacgt
     3481 cagcgccctg tggatcgtgg tcgcctctgt gtcggtgtat ctggtggcgt atcgctacta
     3541 cagtctgtac atcgcccaga aggtgatgaa actcgacccc acgcgcgcga cgcctgcggt
     3601 tattaacaac gacggtctga actacgttcc gaccaaccgt tacgtgttgt ttggtcacca
     3661 cttcgccgct atcgccggtg ctggtccgct ggtcggtccg gttctcgccg cacagatggg
     3721 ccacctgcct ggcacgctgt ggctgctggc gggggtagta ctggccggtg cggttcagga
     3781 ctttatggtg ctgtttatct cctctcgccg taacggcgca tctctcggtg agatgataaa
     3841 agaagagatg ggaccagtac cggggactat cgcgctgttt ggctgtttct taatcatgat
     3901 catcatcctt gccgtcctgg cgctgattgt ggttaaagcc ctggccgaaa gtccgtgggg
     3961 tgtcttcacc gtttgctcaa ccgtaccgat tgcgctgttt atgggtatct acatgcgctt
     4021 tatccgtccg gggcgtgtgg gtgaagtctc tgtcattggt atcgtgctgc tggttgcctc
     4081 tatctacttc ggtggcgtga ttgctcacga tccgtactgg ggtccggcac tgacctttaa
     4141 agacaccacc attaccttcg cgctgattgg ctatgcgttt gtttccgcat tgctgccggt
     4201 gtggctgatc ctcgcaccgc gcgactatct ggcaaccttc ctgaaaatcg gcgttatcgt
     4261 cggcctggcg ctgggtatcg tggtgctgaa cccggaactg aaaatgcctg cgatgaccca
     4321 gtacattgac ggtactggcc cgctgtggaa aggtgctctg ttcccgttct tgttcattac
     4381 catcgcctgt ggtgcggtat ctggcttcca cgcactgatc tcttccggta cgacgccgaa
     4441 actgctggct aatgaaaccg acgcgcgttt cattggctac ggcgcaatgc tgatggagtc
     4501 cttcgtggcg attatggcgc tggttgctgc gtccatcatc gaaccgggtc tttacttcgc
     4561 gatgaacacc ccgcctgctg gccttggcat caccatgcct aacctgcatg aaatgggtgg
     4621 cgagaacgcg ccgatcatca tggcgcagct gaaagacgtt accgcacacg cggcagcgac
     4681 cgtcagctct tggggcttcg tgatttcgcc agagcagatc ctgcaaaccg cgaaagacat
     4741 tggtgagcct tctgtcctga accgtgcagg tggtgcgcca acgctggcgg taggtatcgc
     4801 tcacgtgttc cacaaagtgc tgccgatggc tgacatgggc ttctggtatc acttcggtat
     4861 tctgttcgaa gccctgttca tcctgaccgc gctggatgcg ggtacccgtt ctggccgttt
     4921 tatgctgcaa gacctgctgg gtaacttcat cccgttcctg aaaaaaaccg actctctggt
     4981 tgccggtatc atcggtactg cgggctgtgt gggtctgtgg ggctacctgc tgtatcaggg
     5041 cgtggtcgat ccgctgggcg gcgttaagag cctgtggccg ctgttcggta tctccaacca
     5101 gatgctggca gccgtagcgc tggtactggg caccgttgtg ctgattaaga tgaagcgcac
     5161 ccaatacatc tgggtaactg ttgttccggc tgtatggctg cttatctgca ccacctgggc
     5221 gctgggtctg aaactgttca gcaccaaccc gcagatggaa ggcttcttct acatggcaag
     5281 ccagtacaaa gagaagattg ctaacggtac tgacctgacg gcgcagcaga ttgccaacat
     5341 gaaccacatc gttgtgaaca actacaccaa cgcaggcctg agtattctgt tcctgattgt
     5401 ggtgtacagc atcatcttct acggtttcaa aacctggctt gcggtgcgta acagcgacaa
     5461 acgtactgac aaagaaactc cgtacgttcc aatcccggaa ggcggcgtga agatctcttc
     5521 gcaccactaa ccgtgtttag ccccgcttcg gcggggcttt gttcttatca gagtgaacta
     5581 tgtttggtaa cttaggacag gcaaaaaaat atctcggcca ggcggcgaag atgttgattg
     5641 gcattccaga ctacgacaac tacgtcgagc atatgaagac caaccatccc gacaagccgt
     5701 acatgagcta tgaagaattc ttccgcgaac gccagaacgc gcgctacggc ggcgatggta
     5761 aaggcggtat gcgctgttgt taatggagag agcatgaacc cgattgcagt taccctactc
     5821 accggttttt taggcgcggg caaaaccacc ctgctgcgcc atattcttaa cgaacaacac
     5881 ggctacgaga ttgccgtgat tgaaaacgaa ttcggcgaag tctccgttga tgatcaactg
     5941 attggcgacc gcgccacgca gatcaaaacg ctgaccaacg gctgcatctg ctgttcgcgc
     6001 tccaacgagc tggaagacgc actactcgac ctgctggata atctcgacaa gggcaatatt
     6061 cagttcgacc gtctggtcat tgaatgcacc ggcatggccg atcccggccc gattattcag
     6121 accttcttct cccatgagat tttatgccag cgctacctgc tggacggggt gattgcgctg
     6181 gtggatgcgg tacatgccga tgagcagatg aaccagttca ccatcgccca gtcccaggtt
     6241 ggctacgccg accgtattct gctgacgaaa accgacgtcg caggcgaagc agagaaactg
     6301 cgtgaacgcc tggcgcgcat caacgcccgc gcaccggtct acaccgttac ccacggcgat
     6361 atcgacctgg gccttctgtt caacaccaac ggttttatgc tggaagaaaa cgtcgtcagc
     6421 accaaaccgc gtttccactt tatcgcggac aaacaaaacg atatttcgtc gattgtggtg
     6481 gaactggatt acccggtaga tatcagcgaa gtttcccggg tgatggaaaa cctgctgctg
     6541 gaatcggcgg ataaactgct gcgttacaaa gggatgctgt ggattgacgg cgaacctaac
     6601 cgcctgctgt tccagggcgt ccagcgcctc tacagcgccg actgggaccg cccgtggggc
     6661 gatgaaaaac cgcatagcac gatggtgttt atcgggattc agttgccgga ggatgagatt
     6721 cgggcggcgt ttgcgggact caaaaagtaa ccagccctcc aacatcacaa tacctgagac
     6781 tttatcaccc tcatattcct ccagaatatg agggcattcg ggcttaatta tctctgaacg
     6841 tgcaacactt cctgcgactc cttatgcgcc tccacatcct gcttcgttaa ccagagcgac
     6901 ttacgaccaa aattttcgta cattttcagc tgatcttcat agtgctgatc aactgttcca
     6961 tcgggagcaa taaacccact ctgaccgggt gcgaccacat cccaggcaag caccggacga
     7021 tcgcttgtcg ttggtgagaa aacaatcata tcgttttctg ttccacggtt ttgatactcc
     7081 gcctgatgac gcgtttcttc cgctgcggcc tgcggtacac caaagaaatt atttgcacgg
     7141 aacgttaagg ccattgcagg tgttttccag ttactcacat tattgccata gcgtttggaa
     7201 agagtctccc aggtatcttc cagcgcagcc aacacaacct cctgctgtgg tttcccagca
     7261 aacagatcaa ccgcctgtgg gattggtgat ttgtctccct gcaccgcctc atacaaaatt
     7321 tttgctccaa cacttatatt cagcgaacca gttgggccgt cctgggttgt ttcgtagcca
     7381 ctggcgctgt accacttatc aaatggcata ggtacggcag ccactacggt acgcttcaac
     7441 atactggtca gccaaacgtt caggatggca gagcctggct gctgccaggt tttaccatca
     7501 tcattaagca aattgatgcc atcccaacgt gttaatgtat ccaccaactg acggcgcgga
     7561 tcgctctgtg tcaaaccaga tgtcgctgct tgcagagtag gtaaaaaaag cctcaggtta
     7621 agatcctgac gactggtttg gcgaataaca tcccatgcct gatcagcagt taagcgtggc
     7681 ttttgctcaa gcagtcggtc gatctccgta acgcgatctg ccccgttact ttattcgtac
     7741 cccttataat ggggtgttag ccagccagac ccggcatgat tactgccccc agtcgtcca
//
LOCUS       DAESHI010000166         7774 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.168, whole genome shotgun sequence.
ACCESSION   DAESHI010000166 DAESHI010000000
VERSION     DAESHI010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7774
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.168"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..1780)
                     /locus_tag="KW011_003942"
     CDS             complement(<1..1780)
                     /locus_tag="KW011_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBH7662163.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPERLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     ERINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDS
                     GWGKQVAQEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARR
                     LAAKLGLPFIDAIEKVVDNPPQKMQQN"
     gene            complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="KW011_003943"
     CDS             complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="KW011_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBH7662164.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="KW011_003944"
     CDS             complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="KW011_003944"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBH7662165.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="KW011_003945"
     CDS             complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="KW011_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HBH7662166.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="KW011_003946"
     CDS             complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="KW011_003946"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HBH7662167.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5968..7005)
                     /locus_tag="KW011_003947"
     CDS             complement(5968..7005)
                     /locus_tag="KW011_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBH7662168.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            7339..>7774
                     /gene="yhhY"
                     /locus_tag="KW011_003948"
     CDS             7339..>7774
                     /gene="yhhY"
                     /locus_tag="KW011_003948"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBH7662169.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKY"
BASE COUNT         1976 a         2102 c         1872 g         1824 t
ORIGIN      
        1 ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca tcaataaaag
       61 gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg aggtgcttta
      121 atgaaggaac gcagcatacc caggttggtt caggatgcgg attccagcgt tggcgaacca
      181 tttccgcaca tgcctccacc agctcatcac tgaatcgccc cgtttttttc tcctgtgcta
      241 cctgctttcc ccacccggaa tctccccaac gagaaaggac tcgccccgta gatccttgta
      301 aacccgcagg caagttccct ttaaatccgt attgcgtaaa agctccagat gctacctgtt
      361 tattgagatt taatggcagg tcagcgtgtt taataaataa atttgccgca tggagtaagc
      421 cgctatcaat atcgggcgac aacaacggat gctgaagaca actgctacat ttaccacaac
      481 gttcggcact tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg
      541 tgatatagct ttcaacatct gccagctcat tttttctctg attcatcaaa tggttaattc
      601 gttcatggtc aggagagaaa ctgacgatag ttctgcgcca taatttctcg gtatacacca
      661 caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgga
      721 gattcgtccg ttgttcaatg cctcgtaggg taagaccgtc attctcgcta agtacgttga
      781 gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat ttatgaatag
      841 cgcggtcttc accaccacaa agcaatatgc caactgcgga atctatagca cgcccggcac
      901 gccctacctg ttggtagtaa ccgacaatag accccggcat ttgataatga atgacgaaac
      961 ctaaatcagg tttatcaaac cccataccta atgccgtcgt cgcgaccagc actttgattt
     1021 tatttgccag cagcgcctgc tccagatatt ccctggcagt attagaatct tccgcgcctt
     1081 cacaagtcac gccgctgtag taagcaaatg cactgatacc atttgtcctt aaccactcgg
     1141 cgacaagttc ggcatcgcga gtagtcaggg tataaacaat tcccgatttg gaaaactgag
     1201 ggataaccgt tgctaaccat gccagacggg atgactgttc acctaagact aaggcatcaa
     1261 gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac
     1321 ggatatcctc aacgacacga ttattcgctg tcgcggttgt acccagaaca ggggtattcg
     1381 cagtttcaag aaaatcctga ttcgccaacc gttcaggtga aatcagcaga caatctacac
     1441 caccccgcag caacttatcg ctaatgcgct gccattcttc cctgtttgta gaattcaacg
     1501 tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc agggcaagca
     1561 aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta ctggcgatga
     1621 agtacacggc acttttcccc cagccagtgc gttggacaac cagcagtttt cgccgctgat
     1681 taaccaccgc atctatagct tcccattgcc cttccctgaa agtagcgctg gtgttactta
     1741 acatccgctg taacagcaat tcagctccgt gtttctccat catcttctcc ctcgtccgtt
     1801 aacgcagtga ttagaacgga taatgttccg gtctttttgc gagagagatc aaaaagatga
     1861 agacaggaga taagagaaat gaattactgc gagcaagctc gcagtaattt gtcgcagtaa
     1921 ttaactcaac agctggaacg caatcatccc aacaatggca ccaacagtgc cgaggatggt
     1981 ttccatcatg gtccaggttt tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa
     2041 caaccagaaa ccagcgtcgt taacgtggct gacaacaatc gaaccaccgg cgatacaaat
     2101 cgacagcgcc gccatttgcg caccggaatt gttcagttgt tcaatgaccg gcatcaccag
     2161 ccccaccgcc gttaaacagg caacggtggc agacccctga atgatgcgca ctgcagctgc
     2221 cagcacgaag caggtgatag caatcggcag gcccatgccg gttaacgctt cgcccagtgc
     2281 cggacctacg ccagagtcaa ccagcacctg tttgaacacg ccgcctgcac caatcaccag
     2341 cagaatgatc cccgccggtt gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg
     2401 catgccctga cgcattgcca ggccataaat cgctaccaga caagcaacca gaatcgcggt
     2461 aaacggatga ccaataaact cgaaccattc gtaagcggta gagccttctg gcacaaaacg
     2521 cgcggcaatg gttttcagcc ccaccagcac cagcggcaac aggatcagcg acaggctgaa
     2581 tccgaaagat ggcattttac cttcgccgag atgcggctcg ctgatgtcgt caggaatatg
     2641 cagctcaacg taacggctga tgaagttacc ccacagcggc ccggcaataa tcattcccgg
     2701 aattgccgca cacaggccaa tcaggatcat ccagccaaaa tcggcgttca tctgcgatgc
     2761 cagcagcatt ggcgctggcc ctggcaccag gaacgccgcc gctgccgcca cgcctgcgaa
     2821 taatgggatt atcagcttca ccaggttcgt atcggtgtgg cgcgccattg agaaagcaac
     2881 gctaatcagc agaacaatcg ccacttcaaa gaacagcggt agcgcacaga ccagccccgc
     2941 aaggccgatg gcataatgcg cgcggctgtg accgaaggat ttgagcattt tgacggcaat
     3001 ctgatcgact gcgccggttt catgtaagat cttgccaaac atagctccca gggcgacaac
     3061 caccgccagg aagccgaggg tgcctcccat ccctttttcc atcgtcgctg cgattttatc
     3121 gagcggcata ccggaaaaaa ggccagcccc catggacacc accatcaaag ccaggaaagc
     3181 gtgcatacgc gccttcatga ctaaaaacag cagcagtaaa acagacccta ctgctgttaa
     3241 aacaagcgtt aatgtagtca ctacttattt gcctttttta ataacctcaa tggtgcttgc
     3301 cacaacacct tccagcggtt gatcgatatc caccaccagt acatcggttt cgtccgcacc
     3361 cggctcctgc agcgtttcaa actgcgtcac caacatttgg gttttaaaga aatggccttt
     3421 gcgcgctttc aggcggcttt caatcacgtc aaaatcgcct ttcaggtaga tgaaagagag
     3481 attcggatta ccttcacgca gcaagtcgcg atagtgtttt ttcaatgcag aacagacgat
     3541 cagtgacact ttattagtgc gctgcatagc aaacgcggcg tcgttcagcg cctgcaacca
     3601 gggtttgcga tcgtcgtcat tcagcggttc gccagacgcc attttttcga tattgcgccg
     3661 tggatggagg aaatcgccat caagaaacgc ggcatgaagt tgatgcgcca cttcactggc
     3721 gaccgcagat ttgccgctgc ccgatacgcc catcaagacg taaatgtggt gatcatggtt
     3781 agtcgtgctc aaagtggtgc ccccacaata caagaattaa aaatgttacg ggtaactgtt
     3841 atcggtaaca ttgtccagcc ggacaatatc agaagcaata tccatccgtg ccttaagtgt
     3901 ataagtgtga gctacttcaa atttgtaggc ttaaatagat ccgcccggtg acaaggtgaa
     3961 acctaaatct aacattttcg gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc
     4021 agcgccaata ctgcccatcc gctcacgcgg cgtcagcacg ctcgctaacc gcggctccat
     4081 cacctggcca atgtcatgac cgtggaaacc ggcaatcgcc atatcgtcag gaaccttcaa
     4141 tcccagacgc tgacattcaa acgccgcgcc gaccgccagg tcatcgttgg tacagaacac
     4201 gccatccagc tgcggatatt cccgccgcgc ctggcgaatc agttcaatac cggaagagta
     4261 agaagaagat tgctcaacca tcacgctata cggcaccaga cctgcatcca gcatcgcctg
     4321 ttcgtatccc ttctgtttga tgatagtacg ttcgtcgaga cgcgcgccga gataggcaat
     4381 gtggcaatgc ccgcgagcaa taatggcggt ggtcatctgg cgtgccgctt caaagttatc
     4441 aaaaccgacg gcgatatcga ggcacggcga ctggctgtcc atgagttcca ccaccgggat
     4501 ccccgccact tcaatcatct ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag
     4561 tccgtcgata ttccaggaga gcatggattc gagacgttct tgctccattt ccggtttata
     4621 accgtagtga gccagcatgg tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg
     4681 taatacttcc gcgaaaacct ggttggtgag agaaggtaac aggacgccaa tcgcccggct
     4741 ggtggcgtta gagaggatat cgggcgcacg attgggaata tagcccagtt catcaagagc
     4801 ggcggcaatc ttgccgcgta gagcgacgga aacctgctcc gggttgcgta aaaaacggct
     4861 gaccgtcatt ttggtcacgc ctacacggtc agccacatcc tgaagtacgg gtcttttctt
     4921 tttcatcgtc ctgaaggtac aaaagagata gattctctta gtttaacacg gacgcatcag
     4981 aaccttcccg gggaaaacag tgcttgttta caattattag atatacacta aatcaattgc
     5041 atcgcattgt gctaacgacg cggtaaaacc ccggaagcgt gcatcagcac gcgaccggag
     5101 cgagcgagga agcctgcaaa aaggcaggct tcaaagatgc cgttgagttt taaaccggcg
     5161 gcagatcgaa cagtaacact tcgctgtcgc tatccgcatg gatggagatt gcctgctcat
     5221 cccagattgc cagaccatcg ctggtcgagg ctttcacgcc gttaatggtg acattgcctt
     5281 tcaccacctg gatccagacg cggcgttcag cggcaatctg atgcaccgac tgctcatctt
     5341 tcagcaacgc ccagcggtac agttccatat cctgatgcac tttcaatgaa ccatctcgcg
     5401 catccggcga gagcaccagc tgtttgccct gtacggcatc gaagcgacgc tgttcataac
     5461 gcggcgtaat accgttttct tcaggcatga tccagatctg atacagatgc agacgctcgg
     5521 tgctgcttgg gttgtactct gagtgacgaa tacccgtacc cgcactcata atctggaact
     5581 cacccgccgg aacctgctct ttattgccca tgctgtcctg atgctcaaca gtaccttcca
     5641 gcacgtaggt caaaatttcc atatctttat gcgggtgagt gccgaagccc tgccctgctt
     5701 caatcacgtc gtcgttaatc acgcgcagcg cggagaagcc cataaagttc ggatcgtaat
     5761 agttggcaaa agagaaagta tgccaggagt ccagccagcc atgatttgca tgaccacgtt
     5821 catttgcttt gcgtaagtag atcatttgta ttcgccccat gaatgatttc gatgggctta
     5881 gtgtggaccc gatctgcctg ggatgataga gggtgaaaat tgacccctct gttcaaaaaa
     5941 attgaactat tcgaggggcc atcaaactta cttcgcgaga gtcactgtgg agggagaggc
     6001 ttgctcaaat ccgcgttcaa ggatttccag attggtaaga acttcagatt ccttgacgta
     6061 atttggcgca ccgtgggtga tggtttgata caacgcatca taaacgcgcc cgtaatcgcc
     6121 catctccggc ttcatctctt ctctgaccgt cacgccctcg tcattgacat actccagcac
     6181 accgaccgaa tcatccgctg cgaatcccgg ttcgcccggc ataatattag ccttcaggct
     6241 ggtttcctgc tggtcgatac cgtatttaat aaacgaacct ttcttaccgt gaacgataaa
     6301 tttcggataa tcgattttca ccagatggct ggttttgacg atggctttca ggtcgccata
     6361 aaacagttgc gcttcaaagg tgtcgtcagg attggcttta ttacgcaggc tgcagatgtc
     6421 ataagcgacg tgatccgggc gaccgaacag agaaataatc tggtccatcg tatgcacacc
     6481 aaggccatag aacgcgccat cctgcggcaa cccaggtttg gtttctgcca ccgggcggta
     6541 atagtcaaaa tggctttcca cttcaacaat ctctcccagc ttaccgcttt caatcgcttt
     6601 tttcgccgtc aggaagcagg aatcaaagcg acgattctga tatggcgtga cggtcagccc
     6661 tttgcttttc gccagtgcaa acagctcttt cgcctgcgca agtgtcggag tgaacggttt
     6721 ttcgaccagc acatttttcc cggcttccag cgcgcgcttc gcgtactcga agtggctgtc
     6781 cgcatgggtg cagacaacaa ccaacttaac atcgggatcg tttagcactt cgtcgagatc
     6841 gctggtgaaa tggatatgag agtaaatggg agcctgttct tccggcttcg catggcgacg
     6901 aaaaatatgc gcgacatgcc agctatcctt gcggttaagt acatacggca gatggtaacg
     6961 ggtggtgctt ttgccgaagc caataaaggc gcagttgatg accatgattt cgtccttttt
     7021 aaggtggtta tttacacctt agcgcaaagc ggacgtggtt cctactggag tgcgcataac
     7081 gaacacaagc actcccgtgg ataaattgag aacgaaagat caaaaaaaaa gccagcaccc
     7141 ggctggctaa gtaatactgg aagcaatgtg agcaatgtcg tgctttcagg ttctccgcga
     7201 gggtcttcct gatcgcgaga caataataat cattctcatt cgcacttgtc caacactttt
     7261 tgcaaaaaaa atgcatttga ctcgcatttg aaagtcaatg atgttgaaag ggacatttac
     7321 cccaaagagg acaaaggaat gagtgagata gtaatacgcc acgcagaaac acgggattac
     7381 gaggccatca ggcagattca cgcccagccg gaggtgtatt gcaacacact acaggtgcct
     7441 catccttccg atcatatgtg gcaggagcga ctcgccgatc gtcccggcat caagcaactc
     7501 gtcgcctgta ttgatggaga cgtcgtgggc catctcacca ttgacgtgca acagcaccca
     7561 cgccgcagtc atgttgccga ttttggtatc tgtgtcgact ctcgttggaa gaaccgcggc
     7621 gtcgccagcg ccctgatgcg agagatgatt gaaatgtgcg acaactggtt gcgggtagat
     7681 cgcattgaac taaccgtgtt tgtcgataac gccccggcaa ttaaggtcta taaaaaatac
     7741 ggctttgaaa ttgaagggac tggtaagaag tacg
//
LOCUS       DAESHI010000167         7627 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.169, whole genome shotgun sequence.
ACCESSION   DAESHI010000167 DAESHI010000000
VERSION     DAESHI010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.169"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            143..829
                     /locus_tag="KW011_003949"
                     /pseudo
     CDS             143..829
                     /locus_tag="KW011_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1162..2037
                     /locus_tag="KW011_003950"
     CDS             1162..2037
                     /locus_tag="KW011_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HBH7662170.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            2045..2821
                     /locus_tag="KW011_003951"
     CDS             2045..2821
                     /locus_tag="KW011_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HBH7662171.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            2990..5251
                     /locus_tag="KW011_003952"
     CDS             2990..5251
                     /locus_tag="KW011_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBH7662172.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMPEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
     gene            5320..6495
                     /gene="senB"
                     /locus_tag="KW011_003953"
     CDS             5320..6495
                     /gene="senB"
                     /locus_tag="KW011_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HBH7662173.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(7021..7482)
                     /locus_tag="KW011_003954"
                     /pseudo
     CDS             complement(7021..7482)
                     /locus_tag="KW011_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(7520..>7627)
                     /locus_tag="KW011_003955"
     CDS             complement(7520..>7627)
                     /locus_tag="KW011_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HBH7662174.1"
                     /translation="VEPESYLRYVLDVIADWPINRVGELLPWRVALPTE"
BASE COUNT         2114 a         1781 c         1810 g         1922 t
ORIGIN      
        1 ttttgcgtca gagagcgtct gagagggcac gtacaacggg agttatggta aatggatcgg
       61 gttttcggga aggttcgaca ggatttgccg ttgggtgtag tgtaagcgac tgaaaaacaa
      121 acgcccctga aatcatgtcc agatccggca gaaccagcct catgcgcagt tctggctggt
      181 ttttgacgtt gatcgcgagg gggcagcgat tgactggagc gaccggaacg ctcccgcgcc
      241 gaacatcacc gtaaaaaatc ctgtgaacgg tcatgctcac ctgctctacg cgctcaatat
      301 cgccgtgaga accgcgccgg acagctcggt taaggcgctg aaatacgccg ctgcgattga
      361 acgttcgctg tgtgagaaac tttgtgctga tgtgaactac agcggactga tttgtaaaaa
      421 tccgttccac ctggaatggc aggtgatgga gtggcgagag gaagcctata ccctcgatga
      481 actggctgat tatctcgatt tgagcgcctc agcgcgccgt agcatcgata aacattacgg
      541 gatggggcga aactgccacc tgttcgaaat gacgcgcaaa tgggcttaca gggcgattcg
      601 tcagggctgg cccgaattct cacagtggct tgatgccgtg attcagcgtg tcgaaatgta
      661 caacgcatcg cttcccgttc cgctttcacc gcctgaatgt cgggctattg gcaagagcat
      721 tgcgaaatac acgcacagga acttcacgcc ggaaactttc gcacagtatg tggctgatac
      781 gcacacatta acgtacgttt tcgttccatt ggccctcacc ccccgataac gacccaggta
      841 gcgcgccgta ttccccccgc cggccgtttt cttcgacatg tacatcaggc gcggaactca
      901 tcggggcgcg ggaatgaaat cccattcaga ctccggatag attcaagcaa gcccacttat
      961 cgtcaaaatc ggtgttgcaa aaacgggagt gaccatagat ccgttttcca agcggacccc
     1021 ataatatcgg ctggtaaaca tcactcgcta gtgcattctt tttgttggta atgataatca
     1081 tttacattca ttctttgatg ttttatcata attaatgttt tgtcaataaa tatatggaaa
     1141 catattgttt cagatgggaa tatgagaaag ttcatcctta tttctatgat aacgttagct
     1201 agtgttagtc tgggagcctg cagacagaat gtcaccataa agcaggatgc gccagggcag
     1261 aaagctttcc tgactgacgg gcagatttac gatttgcaca gtggcaaaat tatttctagc
     1321 agcgagcttt tggcggatct tgctaccgct cgacatctta ttattggtga aaaacacgat
     1381 aatgccgagc atcatcagat tgagctttgg cttattcaaa atttgctcat ccagcgacca
     1441 caggggagtg tgttgcttga gatgttgacg tcagagcaac agccccgagt taaccaagtt
     1501 aaatgttggc tgaaagataa tcctgtggtt cgagatagcc gtgttcagga actacttaac
     1561 tggcaaaaag ggtggtcctg ggagatgtat ggcgacatcg tcatgcagct attacgtggg
     1621 ccttacccgc tgctcaatgc caacattggc cgggagcaga tgttggcact gtacaaaaaa
     1681 aacgaatttc ctaaaggaaa aaaatccact gcacctgtgg tacaagaagc tctgcgtgaa
     1741 accatcatat ctatgcatga aggcaacctt gaaagccagc aactgacgtc aatgctctct
     1801 attcagcagc agcgggatcg ctacatggcc cgccagcttc tgagtgcccc ggtaccgtcg
     1861 ttgttgattg caggtggtta tcacgcgtca aaaagcatgg gcgtaccgtt acacatggaa
     1921 gatcttgcca ccggcaccca tccagtggtg ttgatgcttg cagaaaaagg aatgaacatc
     1981 acagtagatc atgctgacta cgtgtggttt gtagcgccag atacaacaaa aaggtaaacc
     2041 atggatgatg aatattctcc acttctcaca gtcggtaaaa tggagcagct ggtttatctg
     2101 ttctctactg cttcatggac tgattttcct tgcgtttatc tggcgttttt ccgaagttca
     2161 gcccgctatg tctcctgcac cggcgatcat gctgcaatgg gcagaaatta tagaggctcc
     2221 ttcttctcca ctttcgcttc cggtgggaat cgctcaacag gagtccgcag tgacagaaga
     2281 aaagcaacaa actgaggaca gacaacaacg tcctgtcact gaagacagtg atgcaaccat
     2341 tgaaataact aggaagaaaa agtcatccga tggagaaaag aaaaagacac gtccacctcg
     2401 taagataaag gctcaaacca gcgacagcaa ccccacagcc gtttccagta atgctgcacc
     2461 tcaggctctg gtcgaatcgt cccgtattgc tgcgcctttt aacagtgact caacaaagcg
     2521 agataattct gaggcatctt gggaaagcag agtaaaaggc cacctaaacc gttataaacg
     2581 ttaccctggc gatgccagaa aacgtgcacg aaccggcaca gccgtcgtga cttttaccgt
     2641 aaacacggaa ggcactattg tcagtagttt cttggaaatc tcttcaggaa ccctttctct
     2701 tgaccgcgaa gctattgctg ttttagaacg cgctcaacca cttcccaaac caccgcctga
     2761 aatattggag ggaggattat ttaaagtaaa aatgccgatt acctttaagt tgaaggagta
     2821 aagggataat tggccgttca agttcattta actgaaattt aattttcatt tttatgctga
     2881 acattttatc aaacttttcc actgtcgaaa aaatcaaaaa gacagtagaa cataattaat
     2941 aaacaaatag ttatacttat ctttattaat attataccca aggaaattta tgaacgttat
     3001 aaaactggct atcggctcag gcatattatt gctcagctgc ggtgcttact cacaatccat
     3061 cagtgaaaaa actaattccg acaaaaaagg agcggcagaa ttcagtccgc tcagcgtttc
     3121 tgtcgggaag acgaccagtg agcaggaagc tctcgagaaa acaggcgcga ccagttcccg
     3181 gacaacggac aaaaacctgc aatcacttga cgcaacagtg cgtagtatgc ctggtactta
     3241 tactcaaata gatcctggtc agggagcaat cagtgtgaat attcgaggca tgagcggatt
     3301 tggtcgtgta aacactatgg tcgatggtat tacccagagt ttttacggaa cctctacctc
     3361 cggaacaacg acgcatggtt caactaacaa tatggctggc gtacttatag atcctaactt
     3421 actggtagca gttgatgtta cacgcggtga cagcagtggc tctgaaggga tcaacgccct
     3481 tgccggtagt gcaaatatgc gtactattgg cgttgacgat gtaatattta acggtaatac
     3541 atatggcctt cgttcacgtt tctctgtcgg tagtaatggg ctgggacgca gcggaatgat
     3601 cgcccttggt ggaaaaagcg acgcttttac ggatacggga agcattggcg ttatggctgc
     3661 tgtgagcggc agttctgtgt actctaattt ctcaaatggt tctggaatta acagcaaaga
     3721 gtttggttat gataaatata tgaagcagaa ccccaaatcc caactgtata aaatggatat
     3781 cagaccagac gaatttaaca gcttcgaact ttccgctcga acctatgaaa ataaatttac
     3841 acgtcgtgat ataaccagtg acgactatta cattaaatat cattacaccc ctttttctga
     3901 attaattgac tttaacgtaa cggccagtac cagtcgcggt aatcaaaagt atcgtgatgg
     3961 ctcgctgtat actttctaca aaacctcagc gcaaaatcgt tctgacgcgc tggatatcaa
     4021 caataccagc cggttcactg tcgcggacaa tgacctggag tttatgctgg gcagcaaact
     4081 gatgcgtacc cgctatgacc ggaccattca ctcagcggcg ggcgacccga aagcgaatca
     4141 ggaatcgatc gagaacaatc cgttcgcacc ctccggccag caggatattt cagcgctgta
     4201 taccgggctg aaggttacgc gcggcatctg ggaggcagat ttcaatctca actacacacg
     4261 taacaggatc acagggtaca agcccgcctg cgattcacgc gttatctgcg tgccacaggg
     4321 tagctacgat attgacgata aagagggtgg cttcaaccct tcagttcagc tttctgctca
     4381 ggtaacacca tggcttcagc cgttcattgg ctacagcaaa tccatgcgcg ccccgaacat
     4441 ccaggagatg ttcttctcta attcaggagg cgcatccatg aacccattcc tgaagcctga
     4501 acgtgcagaa acctggcagg cgggttttaa cattgatacc agagatttac tggtcgaaca
     4561 ggatgccctg cgctttaagg ctctggcgta ccgcagcagg atccagaact acatctacag
     4621 cgagtcttat ctggtttgtt ctggaggtcg taaatgcagt atgcctgagg tgattggcaa
     4681 tgactgggag ggcattagcg atgaatacag cgacaatatg tacatctacg ttaactcggc
     4741 aagcgacgtt atcgcaaagg gcttcgaact ggagatggat tatgatgcag gttttgcttt
     4801 tggccgactc tctttcagcc agcagcaaac agaccagcca acctccatcg ccagcaccta
     4861 ctttggcgca ggggatatga ccgaactgcc cagaaaatac atgacgctgg atactggtgt
     4921 tcgcttcttc gataacgcgt tgaccctggg cactatcata aaatacacag gcaaggctcg
     4981 tcgcctgtcg cctgattttg agcaggacga acataccggc gcaataatca aacaggattt
     5041 gccgcagatc ccaacgatta tcgatctcta tggtacttac gagtacaacc gcaacctaac
     5101 actgaaactt tcggtacaaa acctgatgaa cagagattat tcggaggcgc tgaataagct
     5161 caacatgatg ccaggtcttg gtgacgagac ccacccagcc aattccgcgc gtggcagaac
     5221 atggatattt ggcggggaca ttcgtttctg atgacaattt ccgctaggtt cagacaatac
     5281 gttttctctc ttatgtcaat tttattgcag gaacgaaaaa tgaatatttt cactttatcc
     5341 aaagcaccgc tatacctgtt aatttcacta tttttaccca cgatggccat ggctatcgat
     5401 ccacctgaac gcgaactttc gcgatttgcc ctgaaaacga attaccttca gtcccctgat
     5461 gaaggcgtct atgaactggc gtttgataat gccagtaaaa aggtgtttgc agcagtcacc
     5521 gatcgtgtaa atcgtgaagc caataaaggc tatctgtatt cgtttaattc agattcgctg
     5581 aaagtcgaaa ataaatacac gatgccatac cgggcatttt cgctggcgat aaatcaggat
     5641 aaacatcagc tctatatcgg acacacccag tcagcgtccc tgcgtatcag tatgtttgac
     5701 accccaaccg gcaaactggt aagaaccagc gacaggttaa gttttaaagc ggcaaacgct
     5761 gcagattcgc gttttgagca ttttcgccat atggtttaca gccaggattc cgataccctg
     5821 tttgtgagtt atagcaatat gctgaaaacg gccgagggca tgaagcctct gcataagctg
     5881 ttaatgctcg acgggacgac gcttgcctta aaaggcgagg ttaaggatgc ttacaaaggt
     5941 acagcgtatg gtctgacgat ggatgaaaaa acacagaaaa tctacgttgg cggaagagat
     6001 tacatcaacg aaattgatgc gaaaaatcag acgctgctgc gtaccatccc gttgaaagat
     6061 ccgagaccac aaatcacaag tgtgcagaat ctggcggtgg actccgcttc tgaccgtgcc
     6121 tttgtggtgg tattcgacca tgacgatcgt tccggtacaa aagatggact ctatattttt
     6181 gacttacgcg acggtaaaca gcttggctat gtgcacacag gagccggagc taacgcggtg
     6241 aaatacaatc cgaaatataa cgaactgtat gtcaccaact tcactagcgg caccatcagc
     6301 gtagtggatg ccaccaaata cagcatcacc cgtgaattta acatgccggt ctacccaaac
     6361 cagatggtgt tgtcggacga tatggatacc ctttacattg gcatcaaaga aggctttaac
     6421 cgcgattggg atcctgatgt gtttgtggaa ggagctaaag aacgtattct gagcattgat
     6481 ttgaaaaagt cgtgatcggg gggaaaaaac atgggataaa aactccgtta ttccactaaa
     6541 ctggccggac taggaaaagc aaacatctct gtcctgccgg tacgcctgtt acgccgctcc
     6601 atgaggctga ggaatctcca gaaaacgaga caggcacagc attgtacgat caactgattt
     6661 gtgccaataa ttaaatgaaa tcacagctat gcctgttcat ggggttctag gccggaaccc
     6721 cagatttttc cgattgtttg ttttctaact gattattcag tagttttaga gggctgacgc
     6781 tcagtggaac gaaaactcac gttaagaggt tttgactatg ataaaagtca aagtaggggt
     6841 gagtttggat atggaaatta ttgtactata aggagagcat gaaaacccct ggggcagtca
     6901 ctgaatgagg gaccagcagc ggtaaaataa ccagcataat ttctgcatgg tcgattttta
     6961 taccgtgaca ggctgttttt tatacttggg gtcagtttgg atatcgaaaa ttattgtacg
     7021 ttaaggttgt tttttgacct tgtcgatctt aactgatctc aaacgacgaa acttacctga
     7081 agtcggtatc cggctgtttt tccagatata atcgcctgtc agattgatat gttcccaacc
     7141 taatggtgat aaatgcgata atagttgagc atttattttc atgcccttac gcttcagagc
     7201 atcaaccgct ctttccatgt aaactgtgtt ccataaggaa atagccgccg tcaataaagt
     7261 caatccactg gcgcgataac tttgattttc cggtttccga tctctaatct cccccaatcg
     7321 atgcatgaat acagcacggg ccagagcatt acgggcttct cctttgttca gcccggcctg
     7381 gacacgtcgc cgcaatgccg gatcccgaaa ccagtccagc ataaacagtg ttcgttctat
     7441 acggccaatc tctctcagag ctttagccaa cccattttgt ttgtaagcgt gcagcaagaa
     7501 ccgtattgac gggaatgtgt tattcagtcg gcagtgctac gcgccagggg agcagttcgc
     7561 cgacccggtt tatcggccag tcggctatga cgtcaaggac atagcggagg tagctttctg
     7621 gctccac
//
LOCUS       DAESHI010000168         7611 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.170, whole genome shotgun sequence.
ACCESSION   DAESHI010000168 DAESHI010000000
VERSION     DAESHI010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.170"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            171..701
                     /locus_tag="KW011_003956"
     CDS             171..701
                     /locus_tag="KW011_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HBH7662175.1"
                     /translation="MNDKELIKEIKERISSLDVRDNVERRAYEIALASLTAEPVAWKV
                     TFTQIDREYNTFTGMYSDKAEVERWVRLHKACNFRADITPLYTAQPVPVTPDAWISCS
                     ERMPEMGERQCYVLAADFKNNYPPNIPNTQVGVYGDWFNDGNPTWDDGDGEDLYLKEV
                     THWMQLPEPPQEVNRG"
     gene            780..1502
                     /locus_tag="KW011_003957"
     CDS             780..1502
                     /locus_tag="KW011_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662176.1"
                     /translation="MVNNIANHSSFPAVTIDSQMLLKMVNEARKLYGEPSVRNNKFIE
                     KIEDELEGETYTKSVGRKNGADIDVISMTIKQALRVAARESKAVRRTLVDKLESMQEA
                     HIKSGKSASGLVEYRQARTLKMTVEAVTNLFDLMPNLAPEAKQTAAASIINPLVGFNA
                     IPLPAIEEHYYSAGEVAEQLGVTANKIGRIANANNLKTEQYGKFFLDKSAHSSKQVET
                     FRYNEAGVHKIEELIEGERKAA"
     gene            1824..2447
                     /locus_tag="KW011_003958"
     CDS             1824..2447
                     /locus_tag="KW011_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HBH7662177.1"
                     /translation="MASQLIPVFNGTISNETTLLVNARDLHEFLEVRRDFSTWIKNRI
                     TEYGFAVDVDYILVHQNRGIKGRGGDRRSKDYHLTLDTAKETAMVERNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMENAQDICKRLYPILKELNSSYTSKLYD
                     IAFETFYTVTKNRDVLLREATRLDQASALFERAKPMLKSLRARQFEF"
     gene            2532..2750
                     /locus_tag="KW011_003959"
     CDS             2532..2750
                     /locus_tag="KW011_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBH7662178.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            2728..3798
                     /locus_tag="KW011_003960"
     CDS             2728..3798
                     /locus_tag="KW011_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH7662179.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTKPVAATRAAKSEVRRSRLTADEYLKIYQTAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            3933..5216
                     /locus_tag="KW011_003961"
     CDS             3933..5216
                     /locus_tag="KW011_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HBH7662180.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(5450..>7611)
                     /locus_tag="KW011_003962"
     CDS             complement(5450..>7611)
                     /locus_tag="KW011_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HBH7662181.1"
                     /translation="GTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKAS
                     GMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMRE
                     MMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKP
                     APYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSS
                     VWQTDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYE
                     IDTLNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENV
                     IAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFG
                     AGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSASE
                     LDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVC
                     SKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAG
                     NVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLAN
                     IAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIH
                     EDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
BASE COUNT         2093 a         1863 c         1896 g         1759 t
ORIGIN      
        1 cgctacgcga taagcaggcg ttacttgaag cctcagaaaa gcgcatagca gaactggagg
       61 cacagacagt taccgttaaa gaggttggag atgcttgata gttttgcgct cgttgcactg
      121 gttggcattg tagcgttgat ttgggtgaca tccgggaatc ggaggcataa gtgaacgaca
      181 aagaactgat taaagaaatc aaagagcgta tcagcagttt ggatgtgcga gacaatgttg
      241 aacgccgtgc ttatgaaatt gctctggcat cgctgacagc agagccggtg gcgtggaagg
      301 taaccttcac gcaaattgac cgtgaatata acacgttcac tggtatgtat tctgacaaag
      361 cagaagtcga acggtgggtg cggctgcata aagcatgtaa ttttcgggca gatataacac
      421 cgctttatac cgcccagcca gtgccggtaa ctccggatgc ctggataagc tgtagtgagc
      481 gaatgccaga aatgggagag cgacaatgct atgtgttagc agctgacttt aaaaacaact
      541 acccaccaaa catccccaac actcaggtcg gcgtatatgg cgactggttt aatgatggca
      601 atcccacttg ggatgacggt gatggcgaag acctgtatct caaagaggta acccactgga
      661 tgcagctacc agaaccgccg caggaggtta accgtggcta acctgcaact tgccgtcaaa
      721 ggtgaataac aatcctcgca ctcgcgggga tttcttttat acgatatagg ggaaaaggtg
      781 tggttaacaa tattgcaaat cactcttcgt ttccggctgt aacaatcgac agccagatgc
      841 tgttgaaaat ggtcaatgaa gctcgcaagt tatatggaga gccatcagta cgtaacaaca
      901 aattcatcga gaaaattgag gatgaattgg aaggcgagac ctacacaaaa agtgtaggtc
      961 ggaaaaacgg ggctgacatt gatgttatct ccatgactat caagcaggcg cttcgtgttg
     1021 ctgctcgcga atctaaagca gttcgccgaa cacttgtaga caaacttgaa agtatgcagg
     1081 aagcgcacat taaaagcggt aaatcagcga gtggacttgt tgagtatcgt caagcgcgaa
     1141 cactgaaaat gacggttgaa gctgttacca atctgttcga tttgatgcca aatcttgcgc
     1201 cggaagcaaa gcagactgct gcggcaagca taatcaaccc gctcgttggt tttaatgcaa
     1261 tacctcttcc ggcaatagaa gagcattact actcagcagg ggaggtcgca gagcagctcg
     1321 gagtaacggc aaacaagatt ggtcgcattg ctaacgcaaa caacctcaaa actgagcagt
     1381 acgggaagtt cttcctggat aaatctgcgc attccagcaa gcaggttgaa acatttcggt
     1441 acaacgaggc tggagtgcac aaaatagagg aactcatcga aggagagcga aaggctgcat
     1501 gattttgaca agatagtttt ccccaaatgt ggggaaaagc ccgaatggcg cggcttacag
     1561 caagataagg cctacatgat ttgacaacac cgcattaacg gggctatatt ccgcttcatg
     1621 gtgctgaaca caccttgcaa agcggaaacc gcacccgtca gtcatgcgga ttttttatgt
     1681 ccatttttca gatatggtcg ggtagcgcgt ataccgaaaa acagccgaaa ggttaaggat
     1741 acgggccgac tttgcacggt gttcaagtac ctgaccgccc tgctgaacac agggctatct
     1801 gaacaaatgc aaaggacata aaaatggcca gtcaactcat ccccgtattc aacggcacta
     1861 tatccaacga aacaactctt ctcgttaatg cccgtgattt acacgaattt ttggaagtac
     1921 gtcgtgactt ctctacatgg attaaaaatc gcataacaga atacggcttt gccgttgatg
     1981 tcgattacat tttggttcac caaaacaggg ggatcaaagg acgtggaggc gatcgccgta
     2041 gcaaagacta tcacctcacc ctcgatacag ccaaagaaac ggcgatggtc gagcgtaacg
     2101 aaaaaggccg ccagatacgc cgatacttca tcgagtgcga aaagaaactt cgcagcatgc
     2161 aaccagcgca gcaattcaca gacgaagaaa tcatcctcct ttgctatatg caggtacaga
     2221 tggagaatgc gcaggacatc tgcaaacgtc tgtacccgat attgaaggaa ctgaactcat
     2281 catacacgag caagctgtat gacatcgcgt ttgaaacttt ttacacggtg acgaaaaaca
     2341 gagatgtact gctaagggag gcgacacgac ttgaccaagc aagcgccctt ttcgaacggg
     2401 caaaaccaat gctgaaaagc cttcgggcga gacaattcga attttaatca tcaaaggagc
     2461 ttcggctcct tttttgttgg agaaaataaa ccaatactcg ctcccttgcg agtaattgcg
     2521 gagactttgc gatgtacttg acacttcagg agtggaacgc acgccagcga cgcccaagga
     2581 gccttgaaac agttcgtcga tgggtacgcg agtgcaggat attccctcct ccggttaagg
     2641 atggaagaga gtatttgttc cacgaatcag cggtaaaggt tgacttaaat cgaccagtaa
     2701 caggtagcct tttgaagagg atcagaaatg ggaagaaggc gaagtcatga gcgccgggat
     2761 ttacccccta acctttatat aagaaacaat ggatattact gctacaggga cccaaggacg
     2821 ggtaaagagt ttggattagg ccgagacagg cgaatcgcaa tcactgaagc tatacaggcc
     2881 aacattgagt tattttcagg acacaaacac aagcctctga cagcgagaat caacagtgat
     2941 aattctgtta cgttacattc atggcttgat cgctacgaaa aaatcctcgc cagcagagga
     3001 atcaagcaga agacactcat aaattacatg agcaaaatta aagcaataag gagggggctg
     3061 cctgatgctc cacttgaaga catcaccaca aaagaaattg cggcaatgct caatggatac
     3121 atagacgagg gcaaggcggc atcagccaag ttaatcagat caacactgag cgatgcattc
     3181 cgagaggcaa tagctgaagg ccatataaca acaaaaccgg tcgctgccac tcgcgcagca
     3241 aaatcagagg taaggagatc aagacttacg gctgacgaat acctgaaaat ttatcaaaca
     3301 gcagaatcat caccatgttg gctcagactt gcaatggaac tggctgttgt taccgggcaa
     3361 cgagttggtg atttatgcga aatgaagtgg tctgatatcg tagatggata tctttatgtc
     3421 gagcaaagca aaacaggcgt aaaaattgcc atccctacaa cattgcatgt tgatgctctc
     3481 gggatatcaa tgaaggaaac acttgataaa tgcaaagaga ttcttggcgg agaaaccata
     3541 attgcatcta ctcgtcgtga accgctttca tccggcacag tatcaaggta ttttatgcgc
     3601 gcacgaaaag catcaggtct ttccttcgaa ggggatccac ctacctttca cgagttgcgc
     3661 agtttgtctg caagactcta tgagaagcag ataagcgata agtttgctca acatcttctc
     3721 gggcacaagt cggacaccat ggcatcacag tatcgtgatg acagaggcag ggagtgggac
     3781 aaaattgaaa tcaaataatg attttatttt gactgataat gacctgttcg ttgcaacaaa
     3841 ttgataagcg atgctttttt ataatgccaa cttagtataa aaaagcaggc ttcaacggat
     3901 tcatttttct atttcatagc ccggagcaac ctgtgaacac attttcagtt tcccgtctgg
     3961 cgctggcatt ggcttttggc gtgacgctga ccgcctgtag ctcaaccccg cccgatcaac
     4021 gtccttctga tcaaaccgcg cctggtacct cttctcgccc gattctgtcg gcaaaagaag
     4081 cgcagaattt cgatgctcaa cactattttg catccctgac accaggtgct gcagcgtgga
     4141 atccttcccc gattaccctg cctgcgcaac ctgactttgt tgtcggcccg gcgggcactc
     4201 aaggtgtaac gcataccacg attcaggcgg cggtagatgc ggcaattatc aagcgtacca
     4261 acaagcgcca gtatattgcc gtgatgcctg gtgagtatca gggaacggta tatgtccctg
     4321 ccgctccggg tggaattact ctgtacggta caggtgaaaa accgattgat gtgaagattg
     4381 ggctttccct tgatggtggc atgagccctg ccgactggcg tcacgacgtc aacccgcgcg
     4441 gcaaatatat gccaggtaaa ccagcgtggt atatgtacga tagctgccag agcaaacgca
     4501 gcgacagtat cggtgttctc tgctctgcgg tcttctggtc acaaaacaat ggcctgcaac
     4561 tgcaaaatct gaccatcgaa aacacgctgg gcgatagcgt agatgcaggt aaccatccgg
     4621 cggtggcact gcgtactgat ggtgaccagg tacagattaa caacgttaac attctcggtc
     4681 gtcagaacac cttctttgtc accaacagcg gtgtgcagaa ccgtctggaa acgaatcgtc
     4741 agccgcgtac gctggtgacc aacagctaca ttgaagggga tgtggatatc gtttctggtc
     4801 gcggcgcagt ggtgttcgat aacaccgaat tccgcgtggt gaactcacgt actcagcaag
     4861 aagcgtatgt gtttgcaccg gctacgctgt ccaacatgta ctacggtttc ctcgccgtaa
     4921 acagccgttt caatgctttc ggtgatggtg tggcgcaact gggccgctcg ctggatgttg
     4981 atgccaatac caacggtcag gtggtgatcc gtgatagcgc catcaacgaa ggttttaaca
     5041 cggctaaacc gtgggccgat gcggtgatct ctaatcgtcc gtttgcgggt aataccggca
     5101 gcgtagatga taacgacgaa atacagcgca atctgaatga cactaactac aaccgcatgt
     5161 gggaatacaa taaccgcggc gtgggtagta aagtggttgc agaggcgaag aagtaagagc
     5221 aattaactat ttgccggatg cggcgtaaac gccttatccg gcctacggtt cgatgcgatt
     5281 tgtaggtcgg ataagatgcg caagcatcgc atccgacaat aagtgccgga tgctgcgtaa
     5341 atgccttatc tggcctacag attcgatgcg attcgtaggt cggataagat gcgcaagcat
     5401 cgcatccgac aataagtgcc gaatgcgacc tacattcaca tggcgctttt tacatctgac
     5461 ggtttttatt gaagttaatc aaactacccg ccttgataat ctcgcgctct tcagcagtca
     5521 gactttccat atagagcgta atttccgtta ccggcgcatc ttcatggatc acataacctt
     5581 taaacgtcgt acccggatta tccagcgccg ctttaatgcc agggatgtaa atgtaatccc
     5641 ccacttcaaa ggttggcact tccgccatct gcagaggtaa catcccccag ttgatgacgt
     5701 tagaacggta gcgtttagtc gcgtactcct cggcaatatt cgccagaccg ccaatcacac
     5761 gctggcagct cgccgcctgt tcacgcgcag aaccatcgcc tggtttcacc gcatagacca
     5821 tgctgccaat ttcagtttgc agcggatcaa tatgctcctg accagcaatc tgcttaatgc
     5881 gcgcaaacac ctctgtcagc tcgctgacat tccccgccag acgctgattt tccagctcag
     5941 cagtcgcttt acttctgcca acataaccgg gatcacggcg agacagggta aactccgcca
     6001 gaccaatcgg atttgaacga taagaagagg tttcaccgga aggaatcagt tcgtcggtgg
     6061 tggtcacttc gtcgaggatc ttcgagcaca ctttcaggac gatattgtca gtcagcgcac
     6121 ccaattccgg ccagtcttta atgttcggcc cgtaaatcag cggttgctga gttgcccctt
     6181 tcacaaagcc ctgataaaca cggtttttat acggcgttac atcgaaggcg tactccggca
     6241 cgttgtccca gcaatcaagt tcgctggcag aggttaaata gccaccgttt gccgcagtcg
     6301 cagcgataga acgagcgtcc atcaacgcca ccgctgacat ctgcccatta gctggcttag
     6361 agccttcgcg gttcgggaag ttacgcgtgg tgtggcgaat actcaaaccg ttgttgattg
     6421 gcgtatcgcc cgcgccaaag catgggccgc agaacgcggt tctgatgatt gcgcctgcgc
     6481 caatcaaatc tgctaccaca ccttttttgg cgagatccat aaacaccggc tgtgatgacg
     6541 ggtaaactgc cagcgagaag gtgtcattgc cacaggattg accgcgcagt gcattcgccg
     6601 ccgcgatgac gttttcgtag ttaccgccag aacagcccgc gataatcccc tgctgcactt
     6661 tcaggcgacc attttccact ttatccagca gcgagagttt ggctttaccg tgcgccacgc
     6721 gttcggactc aatttcaatc tcacgcagaa tgtcggtcag gttctggttc agtgtgtcga
     6781 tttcatacac gttgctcggg tggaacggca gcgcaatcat tggtttgatg gcgcttaaat
     6841 caacgctgat gcagccatcg tagtacgcca tcggttgagg gttaagctgg cagtaatcct
     6901 ggccgcgacc gtgcagcgcc agccagttat gcacttcttc atcagtttgc cagacggagc
     6961 ttaaacaggt ggtttccgtg gtcatcacgt caacgctgtt acggaaatcg gtagagagcg
     7021 cgctaacgcc cggtccaacg aactccatga ctttgttttt gacgtaaccg tttttgaaca
     7081 ccgcgccaat gatagccagc gccacatcct gtggccccac atacggcgca ggttttccgg
     7141 tcagatgcac cgcaaccacg cccggatagt cgatatccca ggtgtcatta agcagctgtt
     7201 ttaccaactc accgccaccc tcaccgactg ccattgtccc taatgcaccg taacgggtgt
     7261 ggctgtctga cccgaggatc attttgcccc cgcctgccat catctcacgc atatattgat
     7321 ggatgaccgc aatatgcgga ggcacaaaaa taccgccata acgctgagct gccgataaac
     7381 caaaaacatg gtcatcaccg ttaatggtgc cgccgacggc gcagagtgag ttatggcagt
     7441 tggtcagcac atacggcagc gggaaacgtt ccattccgga cgctttagcg gtctgtacaa
     7501 taccaacaaa ggtaatatcg tgagaggcta atgaatcaaa cttaatttta agtttatcca
     7561 tatttccgga cgtattatga gaagagagaa tagaccaggc aatagtgcct t
//
LOCUS       DAESHI010000169         7491 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.171, whole genome shotgun sequence.
ACCESSION   DAESHI010000169 DAESHI010000000
VERSION     DAESHI010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.171"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(16..522)
                     /gene="mug"
                     /locus_tag="KW011_003963"
     CDS             complement(16..522)
                     /gene="mug"
                     /locus_tag="KW011_003963"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HBH7662182.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            647..722
                     /locus_tag="KW011_003964"
     tRNA            647..722
                     /locus_tag="KW011_003964"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:680..682,aa:Ile,seq:cat)
     gene            complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="KW011_003965"
     CDS             complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="KW011_003965"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HBH7662183.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1828..2451
                     /gene="nfeR"
                     /locus_tag="KW011_003966"
                     /gene_synonym="yqjI"
     CDS             1828..2451
                     /gene="nfeR"
                     /locus_tag="KW011_003966"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HBH7662184.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(2558..4078)
                     /gene="aer"
                     /locus_tag="KW011_003967"
     CDS             complement(2558..4078)
                     /gene="aer"
                     /locus_tag="KW011_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HBH7662185.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4496..5875
                     /gene="ygjG"
                     /locus_tag="KW011_003968"
     CDS             4496..5875
                     /gene="ygjG"
                     /locus_tag="KW011_003968"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HBH7662186.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5917..6249)
                     /locus_tag="KW011_003969"
     CDS             complement(5917..6249)
                     /locus_tag="KW011_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HBH7662187.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6468..7451
                     /gene="ebgR"
                     /locus_tag="KW011_003970"
     CDS             6468..7451
                     /gene="ebgR"
                     /locus_tag="KW011_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HBH7662188.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1823 a         2031 c         1869 g         1768 t
ORIGIN      
        1 tttcggtggc gttttttatc gcccacgcac taccagcgcc tggtccagct cgcgatacgc
       61 ttcaaccagt ttctctagtg aaacgcgact taaaccgctg ggatttggca gcacccaaat
      121 ctgcgtcgaa ccaatggtga gcgtttgttt cccccactgt gcaccgcgct ggctgaatcc
      181 ctgttcatat gcttgtttgc ccagaatcgc caacgcctgc ggctgataat cttcaatttt
      241 ttcaatcagc ttacgcccac ctgcgtgcag ctcctgcttt gaaacttcat tggcttgcac
      301 cgttggacgg tctaccagtt tggtgacgcc acaacgataa tccagcagat gctgtgcctc
      361 ctgcggcttc aactgacggt cggtaaaccc ggcctgatat atcaccttcc agaagcgatt
      421 tgctggatga gcaaagggaa aaccagtccc ggcggatgaa agcccagggt tgataccgca
      481 aaacacgacc cgtaaccctg gagccaaaat atcctcaacc atatttactc ctttctaaca
      541 tcaccaggaa aagtataacg gattgaaaat acattgttta taaaaacagc aggcgcacgg
      601 taatggctgg attgcgacac ggagttactt tataatccgc taccatggcc ccttagctca
      661 gtggttagag caggcgactc ataatcgctt ggtcgctggt tcaagtccag caggggccac
      721 cagatatagc aaaggctgac gagaaatcgt cagccttttt ctttttatat atcagttact
      781 ttgcgtgcca gtaagccgct gcacgtaccc gctgtgggtc atactgttcc gcttcaaagc
      841 ggcggcttaa attcttaacg actttacctt cgccggttat ccagatgaag taatcatcgg
      901 cagggatttg catctgcgcc agacgcgcat ctaccgcctg ctcatcatgt gccagccatt
      961 cgatattaaa accatcaagg tgcgcgagat aatcctgaca ggcgttatcc cgcacgctaa
     1021 ccagcgcact aacttgcggt ttaacggcaa gtttgctcaa cgtttccaag cggcggcgca
     1081 gtgcgggcat tccggattca tcgcagacat acagctgata cgcgtaatct tccggcacca
     1141 ccagcgaacc gcgcggacct gcaaccgtaa gtttatcgcc cggttgcgcc tgcatcgccc
     1201 agccgctggc gaccccaccg tcgtgaataa agaaatcgat cgccagttca tggcgtagtt
     1261 cgtcatacag cggcgtatag tcacgcgacg gtgggcgtgg tccttccggc cagacgatgc
     1321 cctcttccgt taccgttggc ggcacaaagt gagcgtcagg ttgaggaaag aagagtttgc
     1381 tgtgatcgtc aaagccgcgc gatgtaaaac cgtccagcgc ctcgccgccg aggacaatgc
     1441 gctgaaaacc ggcgctgatg cgctcaacgc gtaacacagt cagttcacgg aagcgcagat
     1501 cattgcgaac gcgctgcggg tagcgggggg agttattcat tgttatcgcc ttcgtgatgg
     1561 taatcagata tatctaaata attttcgtaa atgataatga ttgttaatca tgataaatgc
     1621 aagcgatttg tagaactgat atgtctatag tctgataaga cgaaccgcct cttctcaggc
     1681 atcattactc aacgccggat gcggcgtgaa cgccttatcc ggcctacgtg tgagatgagt
     1741 ccacatttaa aaatcatttt tattcttgca agagtgctca tatcagatat aaattagata
     1801 tatctaatca agcaaaagga ggctgatatg agccatcatc acgaagggtg ttgtaaacat
     1861 gaaggccagc cacgccatga gggctgctgc aaaggtgaga agtcagaaca cgagcactgc
     1921 ggacacggtc accagcatga acacggtcaa tgctgcggtg gtcgccacgg tcgcggcggc
     1981 ggtcgtcggc aacgtttctt tggtcacggt gaattgcgtc tggtgattct ggatattctc
     2041 tcgcgcgatg acagccacgg ttacgaattg attaaagcga ttgagaatct aacccagggg
     2101 aattacaccc caagcccggg cgtcatctac ccaacgctgg attttctgca ggagcagtcg
     2161 ctgattacca tccgcgaaga ggaaggtggt aagaagcaga ttgcgctgac cgaacaaggc
     2221 gcgcagtggc tggaagaaaa ccgcgaacag gtggagatga ttgaagaacg catcaaagcg
     2281 cgttgcgttg gtgctgcgct gcgccagaac ccgcaaatga agcgggcgct ggataatttt
     2341 aaagcggtgc tggatttacg cgtcaaccag agcgatatca ctgatgcaca aataaaaaag
     2401 atcattgcgg tgatcgacca cgccgctttt gatattaccc aactggatta atcaccgatt
     2461 gccggatgcg gcgtacccta cacaccccgc aggcctgata agatgcgcca gcatcgcatc
     2521 aggcattgtg ctccaaccgc cggatccggc ataccgatta atgcagcacc gtcaccgcgt
     2581 cttccagtcg gctggcgcgg tgtttcacca tcgccgacac ctgcgcactc tcttccacca
     2641 gctcagcatt tttctgggtg atcaggttaa gctcatccac tgcacgggtc aggctggaaa
     2701 gcccatcggc ctgttccagc gttgaatggc taatctgggc gatcaactgg gtgacgtttt
     2761 tcacctgtgc cacaatatct tccatcgtcc gtccggcggc gtgtacctgc tgcgaaccgg
     2821 attgcacctt atcagcactg gcatcaatca gcttgcgaat atcgttggca gcattagcgc
     2881 tgcggctggc taaatgacgc acttcccctg ccaccactgc aaaacctttg ccctgttcgc
     2941 cggcacgcgc cgcttccacc gccgcattca gggccagaat attggtctga aacgcaatat
     3001 cgttaatcag cgaagtaatg gtgccaatgc gctgggtact gtcggcgata tcgtccattg
     3061 tcttgatcac cgtggtcatc gcctccccac cctgcactgc cgcattactg gcggtgatag
     3121 aaagtttatc ggcagccgac gccgtggcag agttctgttt caccgacgcc gccatttggt
     3181 tcatggtcgc caccgtttgc tgaacgttat caactgtctg ctgggtatgt tcgttcagtt
     3241 catcggtgcc tttcgccagc gtctcactgc catttctgac actggacacc tggcttgaga
     3301 catcgttaat tagccaacgg cacatcaggc caagctgccc taccgcgcgt aatgtcagcc
     3361 ccagctcatc gctgcgattc aggtgctcaa cactattacg ctctccggtc gccaccttca
     3421 gtgcctgacg ggcgacattt tctatcgggc gcacaatctg ccattcaaaa caagcgcttg
     3481 ccaacaatac cactaacaca cagaggaaat acgtcaccac cggggcagca acaaaccaaa
     3541 gcatggccgc cagcaagata aacatcaggg tcatcactcc acgcgcccgc cagcgaagcg
     3601 gtaatgaagg cagtttaccc agccagcctt tacgcaccac caggccttta tgaatacgct
     3661 tactggtacg tccggcgttc agcgctttgt acagcggctc caccgccgca atctcttcat
     3721 ccgtcgcccg ggtacgaatc gacatatagc cactgatttt tccctcgcgc accatcggta
     3781 ccgcattggc ccgcacccaa taatggtcgc catttttgcg gcgatttttc acgatgccgc
     3841 tccagggctc cccttttttc agggtgaacc acatatccgc aaacgccgct tttggcatat
     3901 ccgggtgacg caccatgttg tgcggctgcc cttgtaactc ttgcaaggta aagccgctca
     3961 cctgcacaaa agtgtcatta gcatgagtga tatagctttg cagatcggta gtggacatca
     4021 gagtggtatc gtccgccagc ggggtatttt gctgggtgac atacggatga gaagacatgt
     4081 tcgcgtcctg tgcaggttat atggttgtta acttcttgtc agagtttatg tcggccccgc
     4141 tgcggttatc tttaaccgat taatttgatt tagatcgcaa tttgcgatta aagcacaaat
     4201 ctaattcttt gatttaaaat actttcactc tgttactata cgaaaacgtt aattatcttg
     4261 cccaaaaatc aggcaattat tgccctgaaa acgtgcattt gcgcagcaat catcaaatcc
     4321 atacccgaca aaaaccgtgc aaaataacaa caaatgttaa cagatagcat taaatattgc
     4381 acaaatgata accgaatttg tgtttatccc gattttcgcg atcgcagccg gagtggcgca
     4441 atccctgcaa tacttaaatc ggtatcatgt gatacgcgag cctccggagc atattttgaa
     4501 caggttacct tcgagcgcat cggctttagc gtgcagcgcc cacgccctga atctcattga
     4561 gaagcgaacg ctggatcatg aggagatgaa agcacttaac cgagaggtga ttgaatactt
     4621 caaagagcat gtcaatccgg ggtttttaga gtatcgcaaa tctgttaccg ccggcgggga
     4681 ttacggagcc gtagagtggc aagcgggaag tttaaatacg cttgtcgaca cccagggaca
     4741 ggagtttatc gactgcctgg gaggttttgg aattttcaac gtggggcacc gtaatccagt
     4801 tgtggtttcc gccgtacaga atcaacttgc gaaacaaccg ctgcacagcc aggagctgct
     4861 cgatccgtta cgggcgatgt tggcgaaaac ccttgctgcg ctaacgcccg gtaaactgaa
     4921 atacagcttc ttctgtaata gcggcaccga gtccgttgaa gcagcgctga agctggcgaa
     4981 agcttaccag tcaccgcgcg gcaagtttac ttttattgcc accagcggcg cgttccacgg
     5041 taaatcactt ggcgcgctgt cggccacggc gaaatcgacc ttccgcaaac cgtttatgcc
     5101 gttactgccg ggcttccgtc atgtgccgtt tggcaatatc gaagccatgc gcacggctct
     5161 taacgagtgc aaaaaaaccg gtgatgatgt ggctgcggtg atcctcgaac cgattcaggg
     5221 tgaaggtggc gtaattctgc cgccgccggg ctatctcacc gccgtacgta agctatgcga
     5281 tgagttcggc gcactgatga tcctcgatga agtacaaacg ggcatggggc gcacgggcaa
     5341 gatgttcgcc tgcgagcatg agaacgtaca gccggatatc ctctgccttg ccaaagcgct
     5401 cggcggcggc gtgatgccga ttggcgcgac catcgccact gaagaggtgt tttcagttct
     5461 gttcgacaac ccattcctgc ataccaccac ctttggcggc aacccgctgg cctgtgcggc
     5521 ggcgctggcg accatcaatg tgttgctgga gcagaactta ccggctcagg ctgatcaaaa
     5581 aggcgatatg ttgctggacg gtttccgtca actggcgcgg gaatatcccg atctggtaca
     5641 ggaagcgcgt ggtaaaggga tgttgatggc gattgagttt gttgataacg aaatcggcta
     5701 taactttgcc agcgagatgt tccgccagcg cgtactggtg gccggaacgc tcaataacgc
     5761 caaaacgatc cgcattgaac cgccactgac actgaccatt gaacagtgtg aactggtgat
     5821 caaagcggcg cgtaaggcgc tggcggccat gcgagtaagt gtcgaagaag cgtaatatca
     5881 aatcggatgg cgatacgacg tcgccatccg atctttttat acgacgcgta cgcccgcagg
     5941 catcatccgc tccggcgtta acagcacgct ttcactgccg tcatcggttt ccgcacacaa
     6001 cagcatgcat tccgacgttt caccacgcat tttcgctttt tgcagattgc ataagaccac
     6061 caccgttttt cccatcagct cctcttcgct gtagtacggc accaggctgg tcacggtttg
     6121 cagtgttttt tcccccacat caacctgtac gatgtacagc ttgtcggcgt tttcatggcg
     6181 tttcacttcc acaatctttc cgacgcgcat ttccagacgt gcaaaatcag cgtaagccac
     6241 ggtttccatt gcttcctccc ttcaagtaaa attttactaa actatagcaa aattttttct
     6301 caatcctgta ggctaaaaat ggagaatgca ggcgtgatca cattcgtaag ccgctgtgtt
     6361 accgttacag cgtcaaagaa acgcgcttta tttactgaaa acaggtgacc cgataagcac
     6421 ttcctctaca atgggggcgc acatcaggga aagtaaaaaa ggtaaacatg gcaacattaa
     6481 aagacatcgc aatcgaagct ggcgtatccc tggcgacagt atccagggtc ttaaatgacg
     6541 atccgacatt gaatgtgaaa gaagagacga aacatcgcat tctcgagatc gccgaaaagc
     6601 tggagtacaa gaccagtagc gcccgtaaac tacagacagg cgcagtcaac caacaccata
     6661 ttctggctat ctacagctac cagcaggagc tggagatcaa cgatccttac tatctggcga
     6721 tccgccacgg cattgaaact cagtgcgaaa agctggcgat cgaactgacc aactgttatg
     6781 aacacaacgg attaccagac attaaaaacg tcaccggtat tttaattgtc ggcaaaccca
     6841 cgcccgccct gcgcgccgct gccagcgcgt tgaccgacaa tatctgtttt atcgactttc
     6901 acgaacccgg cagcggttac gatgcggtgg atatcgatct ggcacgcatc agtaaagaaa
     6961 tcatcgactt ctatatcaac cagggcgtta atcgtattgg ttttattggc ggtgaagatg
     7021 agcctggcaa ggcggatatt cgtgaggtcg cctttgcgga atatggccga ctgaaacaag
     7081 tggtacgcga agaggatatc tggcgcggcg gtttttccag ttcgtcgggt tatgaactgg
     7141 caaaacaaat gctggcgcgg gaagactatc cgaaggcact gtttgttgct tccgattcca
     7201 ttgctatcgg cgtactgcgg gcaattcatg aacgtggcct gaacatccca caggatattt
     7261 cgcttttcag cgttaacgat atccccaccg cgcgatttac ctttccgccg ctctccaccg
     7321 tgcgcatcca ttccgagatg atgggaagtc agggcgttaa cctggtgtat gaaaaagccc
     7381 gcgatggtcg cgcgctgccg ctgctagtct tcgttcccag caaattaaaa ctgcgcggca
     7441 cgacccgtta aatcccctta cacactgtcc ggcaatcatt tttgccggac a
//
LOCUS       DAESHI010000170         7440 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.172, whole genome shotgun sequence.
ACCESSION   DAESHI010000170 DAESHI010000000
VERSION     DAESHI010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7440
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.172"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(99..1607)
                     /gene="putP"
                     /locus_tag="KW011_003971"
     CDS             complement(99..1607)
                     /gene="putP"
                     /locus_tag="KW011_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HBH7662189.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            2029..5991
                     /gene="putA"
                     /locus_tag="KW011_003972"
     CDS             2029..5991
                     /gene="putA"
                     /locus_tag="KW011_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HBH7662190.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(6031..6669)
                     /gene="rutR"
                     /locus_tag="KW011_003973"
     CDS             complement(6031..6669)
                     /gene="rutR"
                     /locus_tag="KW011_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HBH7662191.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            6957..>7440
                     /locus_tag="KW011_003974"
     CDS             6957..>7440
                     /locus_tag="KW011_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HBH7662192.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPVYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     D"
BASE COUNT         1776 a         2063 c         1946 g         1655 t
ORIGIN      
        1 ctgcgcgctc cctgcaccat tcaatttata tggcgaggca cattaaggga gcgcaacccg
       61 ctgctgaagc caaacaaaac cgccgcaggc taagtccctt agttttcctg caaccgtgac
      121 ggcggagccg aatgatagtg cgcatcggcc tcggcaaagc gtttttgcat cgccgctgac
      181 ggcgctttac ccagcaaact aaacactaca atcccgatac tgccgaagat gaagcccgga
      241 ataatttcgt acagtcccag ccagccgaac tgcttccaga cgataactgt cagcgcaccg
      301 atgatcatcc ccgccagcgc accgttgcgc gtcatgcgtg accacatcac cgagaacagc
      361 accaccggac caaacgccgc gccaaagcct gcccacgcgt agctcactaa gcccagcacg
      421 cggttttccg ggtttgccgc cagcgcaatc gccaccagcg ccaccaccag caccatcaca
      481 cgtcctaccc acaccagctc tttctggctg gcctgtttac gcagaaacgc tttgtataaa
      541 tcttcggtaa tcgcactgga gcacaccagc agctggcaac ttaaggttga cattaccgcc
      601 gccagaatcg ccgacagcag aatcccggca atccacgggt taaacagaat ttgcgccagt
      661 tcgataaaca cgcgttcagc gttctggttt accgcacctg ctaccgccgg atgctcgtta
      721 aagtaagcga taccaaagaa gccaacagcc accgcccctg ccaggcaaag gatcatccag
      781 gtcatactga tacgacgcgc atggacaatg ctgtggtgag aatctgccgc cataaaacgc
      841 gccaggatgt gcggctgccc gaagtaaccc agcccccaac ccatcaatga gataatggca
      901 acaaagttca gccctttgag catatccacg ttttcgatgc tcttttgttt gatcacttcc
      961 agcgagtcac caaagccacc gacactgata atgacgataa ccggcgtcag gatcagggca
     1021 aaaatcatca ggctggcctg tacagtgtca gtccagctca ccgcgaggaa accgccaata
     1081 aaggtgtaaa ggatcgtcga cgcagctccc gccaacagag ccgtttcgta actcatgcca
     1141 aaggtacttt caaacagacg cgcgcctgcc acaatgcccg aagcgcaata aatggtgaag
     1201 aacagcaaaa taaccagcgc ggagataatg cgcaaaatgc ggcttttatc ttcaaaacgc
     1261 ccggtgaaat aatccggcaa tgttaaggcg ttattattgt attcggtatg cacacgcaac
     1321 cgcccggcca ccagcttcca gttgatccac gcgcctaatg tcaggccaat ggcgatccag
     1381 ctttcggaaa tcccggaaag aaaaaccgcg ccaggcaacc ccattaacag ccagccgctc
     1441 atatccgacg cacccgccga taatgccgtc acgaatggcc caagactacg accgcccaga
     1501 atatagtcgt caaagttttt cgttgatcgc caggcgataa acccaatcaa tatcatgcca
     1561 aagatataga cacaaaatgt caccaacatc ggtgtgctaa tagccatcta aagtctccaa
     1621 aaaattatta tcggcaatgt cgaaacttgc cgttatatct gccaccggaa cggggtaaca
     1681 gagtttatgt tttaccaggg cgaccgtatc ctgccggaag cgctggttat tcacaatcga
     1741 tttaacacac catttacatt aaattttagt gctcagcgac actatttttc atcaggttgc
     1801 actctctcac attttttgcg gttgcacctt tcaaaaaggt taactgccgc agagaaaaag
     1861 tctgagttat tttttaatca ctgtcatatc gatttctttt attaacattt cattcatttt
     1921 taagcttgct acgcaagtca catttaacat ggttgcacaa agttgcaaca ttatggatat
     1981 tttacgataa cgttaagttg cacctttcag aacaacagga gtaatggcat gggaaccacc
     2041 accatggggg ttaagctgga cgacgcgacg cgtgagcgta ttaagtctgc cgcgacacgt
     2101 atcgatcgca caccacactg gttaattaag caggcgattt tttcttatct cgaacaactg
     2161 gaaaacagcg atactctgcc ggagctacct gcgctgcttt ctggcgcggc caatgagagc
     2221 gatgaagcac cgactccggc agaggaacca caccagccat tcctcgactt tgccgagcaa
     2281 atattgcccc agtcggtttc ccgcgccgcg atcaccgcgg cctatcgccg cccggaaacc
     2341 gaagcggttt ctatgctgct ggaacaagcc cgcctgccgc agccagttgc tgaacaggcg
     2401 cacaaactgg cgtatcaact ggccgataaa ctgcgtaatc aaaaaaatgc cagtggtcgt
     2461 gcaggaatgg tccaagggtt attgcaggag ttttcgctgt catcgcagga aggcgtggcg
     2521 ctgatgtgtc tggcagaagc gttgttgcgt attcccgaca aagccacccg cgacgcgtta
     2581 attcgcgaca aaatcagcaa cggtaactgg cagtcacaca ttggtcgtag cccgtcactg
     2641 tttgttaatg ccgctacctg ggggctgctg tttactggca aactggtttc cacccataac
     2701 gaagccagcc tctcccgctc gctgaaccgc attatcggta aaagcggtga accgctgatc
     2761 cgcaaaggtg tggatatggc gatgcgcctg atgggcgagc agttcgtcac gggtgaaacc
     2821 atcgcggaag cgttagccaa tgcccgcaag ctggaagaga aaggtttccg ttactcttac
     2881 gatatgctgg gcgaagctgc actgactgcc gcagacgcac aggcgtatat ggtttcctat
     2941 cagcaggcga ttcacgccat tggtaaagcg tctaacggtc gtggcatcta tgaaggtccg
     3001 ggcatttcaa tcaaactgtc ggcgctgcat ccgcgctaca gccgcgccca gtatgaccgg
     3061 gtaatggaag agctttaccc gcgtctgaaa tcactcaccc tgctggcgcg tcagtatgat
     3121 attggtatta acatcgatgc cgaagaggcc gatcgtctgg agatctccct cgatctgctg
     3181 gaaaatctct gtttcgagcc ggaactggca ggctggaacg gcatcggttt tgttattcag
     3241 gcttatcaaa aacgctgccc gttggtgatc gattacctgg ttgatctcgc cacccgcagc
     3301 cgtcgccgtc tgatgattcg cctggtgaaa ggcgcgtact gggatagtga aatcaagcgt
     3361 gcgcagatgg acggccttga aggctatccg gtttataccc gcaaggtgta taccgacgtt
     3421 tcttatctcg cctgtgcgaa aaagctgctg gcggtgccga atctaatcta cccgcagttc
     3481 gcgacgcaca acgcccatac gctggcagcg atttatcaac tggcaggtca gaactactat
     3541 ccgggtcagt acgagttcca gtgcctgcat ggtatgggcg agccactgta cgagcaggtc
     3601 accgggaaag ttgccgacgg taaacttaac cgaccgtgtc gtatttatgc cccggttggc
     3661 acacatgaaa cgctgctggc gtatctggtg cgtcgcctgc tggaaaacgg tgctaacacc
     3721 tcgtttgtta accgtattgc cgacacctct ttgccactgg atgaactggt cgccgatccg
     3781 gtcactgctg tagaaaaact ggcgcaacag gaagggcaaa ctggattacc gcatccgaaa
     3841 attcccctgc cgcgcgatct ttacggtcac gggcgcgaca actcggcagg gctggatctc
     3901 gctaacgaac accgtctggc ctcgctctcc tctgccctgc tcaatagtgc actgcaaaaa
     3961 tggcaggcct tgccaatgct ggaacaaccg gtagcggcag gtgagatgtc gcccgttatt
     4021 aaccctgcgg aaccgaaaga tattgtgggc tttgtgcgtg aagccacgcc acgtgaagta
     4081 gaacaggcgc tggaaagtgc ggttaataac gcgccaatct ggtttgccac gcctccggct
     4141 gaacgcgcgg cgattttgca tcgcgctgcc gtgctaatgg aaagccagat gcagcaactg
     4201 attggtattc tggtgcgtga agccggaaaa accttcagta acgccattgc cgaagtgcgc
     4261 gaagcggtcg attttctcca ctactacgcc ggacaggtgc gggatgattt cgctaacgaa
     4321 acccaccgtc cattagggcc tgtggtgtgt atcagtccgt ggaacttccc gctggctatt
     4381 ttcaccgggc agatcgctgc cgcactggcg gcaggtaaca gcgtgctggc aaaaccggca
     4441 gaacaaacgc cgctgattgc cgcgcaaggg atcgccattt tgctggaagc gggtgtaccc
     4501 cctggcgtgg tgcaattgct gccaggtcag ggggaaaccg tgggcgcgca actgacgggt
     4561 gatgatcgcg tgcgcggggt gatgtttacc ggttcaaccg aagtcgctac gttactgcag
     4621 cgcaatatcg ccagccgcct ggacgctcag ggtcgcccta ttccgctcat cgctgaaacc
     4681 ggcggcatga acgcgatgat tgtcgattct tcagcactga ccgaacaagt cgtcgtggat
     4741 gtgctggcct cggcgttcga cagtgcgggt cagcgttgtt cggcactgcg cgtgctgtgc
     4801 ctgcaagatg agattgccga ccacacgttg aaaatgctgc gcggtgcaat ggccgaatgc
     4861 cggatgggta atccgggtcg cctgaccacc gatatcggtc cggtgattga tagcgaagcg
     4921 aaagccaata ttgagcgcca tattcagacc atgcgtagca aaggccgtcc ggtgttccag
     4981 gcggtgcggg aaaacagcga agatgcccgt gaatggcaaa gcggcacctt tgtcgccccg
     5041 acgctgatcg aactggatga ctttgccgaa ttgcacaaag aggtctttgg tccggtgctg
     5101 catgtggtgc gttacaaccg taaccagcta ccagagctga tcgagcagat taacgcttcc
     5161 ggctatggtc tgacgcttgg tgtccatacg cgtattgatg aaaccatcgc ccaggtcact
     5221 ggctcggccc atgttggtaa cctgtatgtt aaccgtaata tggtgggcgc agtggttggt
     5281 gtgcagccgt tcggcggcga agggttgtcc ggtaccgggc cgaaagcagg cggtccgctc
     5341 tatctctacc gtctgctggc gaatcgcccg gaaagtgcgc tggcagtgac gctcgcgcgt
     5401 caggatgcag agtatccggt cgatgcacag ttgaaagccg cattgactca gccgctaaat
     5461 gcactgcggg aatgggcagc aaatcgtcca gaattgcagg cgttatgtac gcaatatggc
     5521 gagctggcgc aggcaggaac acaacgattg ctgccggggc cgacgggtga acgcaacacc
     5581 tggacgctgc tgccgcgtga gcgcgtgttg tgtattgccg atgatgagca ggatgcgctg
     5641 actcagctcg ccgccgtgct ggcggtgggc agccaggtac tgtggccgga tgacgcgctg
     5701 catcgtcagc tagtaaaggc attgccatcg gcagtcagcg aacgtattca actggcgaaa
     5761 gcggaaaata taaccgctca accctttgat gcggtgatct tccacggtga ttcggatcag
     5821 cttcgcgcat tgtgtgaagc cgttgctgcg cgggatggcg caattgtttc ggtgcagggt
     5881 tttgcccgtg gcgaaagcaa tatccttctg gaacggctgt atatcgaacg ttcgctgagt
     5941 gtgaataccg ctgccgctgg cggtaacgcc agtttaatga cgatcggtta aacaggtagc
     6001 cggaggatgt tacaacctcc tccggcatct ttaacgtggt cgaatcccct caataataat
     6061 ccgctgcacg ttttcaaccg tttgattgaa aaatatctca tcgcgcaacg tcgcgcctgt
     6121 caccgcctcc acctgagggg cgaaatcggc gtaatgttga gtggaagccc aaatcatgaa
     6181 aatcaaatgc tgcggatcaa tcggcgcgag tttgccgctt ttgacccaac cggcaatcag
     6241 cgcagatttc tcatcaatta atgccttcaa atcgcccgtc agttcatcca ttaacagcgg
     6301 cgcgcctgcc agcatctcca tacagaacag gcgcgaagcc tgcggataat cgcgggagac
     6361 ttccagcttc agacggatgt actcttttat cgccgccagc ggagcgaaat cttcacgaaa
     6421 cgcttttaac ggtgccagcc agatatcgag aatctgccgc agcacggcaa tatacagcgc
     6481 ctcttttgac ggaaagtaat acagcagatt ggtttttgaa acacccgcca actctgcgat
     6541 ctgctccagc cttgtgccgt gaaaaccgaa ttgtgaaaaa gtgtccagtg ctgcgctaag
     6601 aatcgctttt ttcttcgcgc ttactgcgcg cgaacgttta cccgttgttt tcactgcgcc
     6661 ttgcgtcatg cgctctcccc tcttgtagat tggcaacagg atagcaaaac ccccgcccgg
     6721 cctcgacctt ctgcactctc atcgcgcaca gatgcatgtt ttatgtgcaa ctgttttgac
     6781 cgtttagtcc actttttatc agatatttta gataacgtaa tattaacata ttaataacat
     6841 taacttttta aaaactggca tccgctttgc aaacaagcca atacgcggct gaaaaggata
     6901 tgcaggatgc agcgccccgt ctgactttca ctttacgaga cgaagagagg ttgatgatga
     6961 aaattggcgt attcgtacct attggcaaca acggctggct catttcgacc cacgcgccgc
     7021 agtacatgcc gacctttgaa ctgaataaag ccattgtgca gaaagcggag cattaccatt
     7081 tcgatttcgc cctgtcgatg atcaaactgc gtggctttgg cggcaaaact gagttctggg
     7141 atcacaacct tgagtcgttc accttgatgg cgggtctggc agccgtgacc tcgcgcattc
     7201 agatttacgc cactgctgcc accttaacgt tacctccggc aatcgtcgcc cgtatggccg
     7261 caaccatcga ctccatctct ggcgggcgtt ttggcgtcaa cctcgtgact ggctggcaaa
     7321 agcccgtgta tgagcagatg ggtatctggc ctggcgatga ctatttctcc cgtcgttacg
     7381 actatctcac cgaatatgtt caggtgctgc gcgacctgtg gggcacgggg aaaagcgatt
//
LOCUS       DAESHI010000171         7274 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.173, whole genome shotgun sequence.
ACCESSION   DAESHI010000171 DAESHI010000000
VERSION     DAESHI010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7274
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.173"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(10..165)
                     /locus_tag="KW011_003975"
     CDS             complement(10..165)
                     /locus_tag="KW011_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HBH7662193.1"
                     /translation="MNTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(368..775)
                     /locus_tag="KW011_003976"
     CDS             complement(368..775)
                     /locus_tag="KW011_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH7662194.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            852..1079
                     /locus_tag="KW011_003977"
     CDS             852..1079
                     /locus_tag="KW011_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH7662195.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPWKVIHENQA"
     gene            1063..1484
                     /locus_tag="KW011_003978"
                     /pseudo
     CDS             1063..1484
                     /locus_tag="KW011_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1562..2349
                     /locus_tag="KW011_003979"
                     /pseudo
     CDS             1562..2349
                     /locus_tag="KW011_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2356..3102
                     /locus_tag="KW011_003980"
     CDS             2356..3102
                     /locus_tag="KW011_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH7662196.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKLGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEVMKTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            3117..3539
                     /locus_tag="KW011_003981"
     CDS             3117..3539
                     /locus_tag="KW011_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBH7662197.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            3597..3953
                     /locus_tag="KW011_003982"
     CDS             3597..3953
                     /locus_tag="KW011_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662198.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            4122..4787
                     /locus_tag="KW011_003983"
     CDS             4122..4787
                     /locus_tag="KW011_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBH7662199.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            5015..5170
                     /gene="hokD"
                     /locus_tag="KW011_003984"
     CDS             5015..5170
                     /gene="hokD"
                     /locus_tag="KW011_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBH7662200.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            5420..5707
                     /locus_tag="KW011_003985"
     CDS             5420..5707
                     /locus_tag="KW011_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662201.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            5746..6345
                     /locus_tag="KW011_003986"
     CDS             5746..6345
                     /locus_tag="KW011_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HBH7662202.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVCN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTSDTALLKSFEAFREWVAIQAGFYTEHFYPDGSRGCRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            6345..6635
                     /locus_tag="KW011_003987"
     CDS             6345..6635
                     /locus_tag="KW011_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBH7662203.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            6632..7186
                     /locus_tag="KW011_003988"
     CDS             6632..7186
                     /locus_tag="KW011_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HBH7662204.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT         1944 a         1554 c         1988 g         1788 t
ORIGIN      
        1 tcgttcattt tactcacctg aatgtcttcc caaccaacga cgtgcgccag cttcggtttt
       61 aaacgttttg cttttggtat acgtcatggc ggtgaatgtg ccgtcctggt tggggaacac
      121 gccgtacacc agagattcgt tgttgccaag atcgatagta ttcatgttga ccccatttcc
      181 ccttaacgcc ggggtagcgg aactgtttgc tgagaacacc gtgcggtgtc ttgatgaatg
      241 aaatttagaa taacctaagg tgggcggtca agatttttgt gtagaaaaac ctaagttttt
      301 tgatgtaaaa aacacaagtg tttgaaagtt tgtgcttttt attacagggt gtggagaaaa
      361 aaggggatta tttgtttgcg cttcttttgc gagctttgag tagttcttca aaaagtttgt
      421 tgaaattttc aactcgagca cgcatctctg acaacagggc cttttgctct gactcaggca
      481 gtgcgtcgaa cagttgaagt aactcttttt gatcttctgt cagaatggct ggctgattat
      541 ccgggatcgg ttcgcctggt tgtttatctt catccccaaa aagaagccaa gtcggtgagc
      601 actgaagcgc ttggctcagt gcgaataatc ttttccccgc cggctgtgtt tcatctcttt
      661 cccattgaga aattgttacg tgagccactt tgaccagctt acctaatgcg gcctgagaca
      721 gttttaattt tttacgccta tgtaagaggc gagcaccgaa ggtttcgttt ttcatattag
      781 ggaattctaa tttttcttga cttaggtttc tctacgatct agtttcctta ggaaaatcta
      841 aggagctcga tatgttgaaa attgatgcta tagcgttttt tggcagcaaa acaaagcttg
      901 ccaatgccgc aggagttagg ctggcaagca ttgctgcatg gggggaactg gttcctgaag
      961 gtcgcgcgat gcgcctgcaa gaggcatctg gcggggaact tcagtacgac cccaaagttt
     1021 atgacgaata tcgtaaggca aaacgacctt ggaaggtgat tcatgaaaat caggcatgag
     1081 cacatcgaat cagtgctgtt gccctggcag ccgaaaaagg gcaggcgtgg gtcgctaacg
     1141 caattactga agaatatctg cggcaggggg gcggcgaatt gtctctggta ccaggcaagg
     1201 actggaacaa tcagcagaat atctatcacc gttggttgaa aggtgaaacg aaagcgcaaa
     1261 gggaaaaaat tcagaaactg atccctgcgg ttctggcaat tcttccgcgt gagctgcgtc
     1321 accgactctg tatcttcgat accctggaac gccgtgcatt actggcggcg caggaagcgt
     1381 tgagtacggc aattgatgcg catgatgatg ctgtccaggc cgtttaccgg aaagcacatt
     1441 tcagcggtgg tgggtcgccc ggcgattctg tcgtagtgca ttgattgaaa ttaatcgtgc
     1501 cggattgttt tgttcggtat cagttaaatg taacgctgcg agcgttacaa ggtgaaaaca
     1561 aatggcttca aactggataa agctcgaggt tattacgccg gataagccgg aaatattcag
     1621 gcttgctgag attctgaata ttgatccaga tgccgcatta gggaaggtta ttcgcttctg
     1681 ggcatgggcg gatcaacaaa tgatagacgg taatgcagat tgtaacgctc gcggcgttac
     1741 aaaaagtgca atagatcgca tcacttttat gtctggtttt gctgatgcgt taattcaggt
     1801 tggatggctg gtcgaaaatg acggtgggct ttctctacct aactttgaac gtcataacgg
     1861 aaaaagctct aaaaaaaaac gggcggttac aaacgagcga gtaacaaaaa tacgcgaact
     1921 gaaacgaaaa ggtaacgctg ccagcgttac acaaacggat caaaaagcgt taccagagga
     1981 agaggaagag gaagaggaag atctaaatac tgatctcccc ctcgccaaaa acgagcgtct
     2041 aaaaaattcg agccggaggc tattgagctg cccgattggt tgccggaaac actctggcat
     2101 gagtgggtcc ggttcagaca ggcattgcga aaaccgattc gaacggagca gggcgctaac
     2161 ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga gcaggtgatt
     2221 cgacacagca tcgccaatga ataccagggc ctgttcgcgc cgaaaggtgt tcggcctgag
     2281 acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc gccaggcttc
     2341 agggggtaac ggaccatgaa aaatattgcg acaggcggcg ttctggaacg catccgccga
     2401 ctgaccccgc cacatgtaac cgccccattc agaacggtag cggagtggcg cgagtggcaa
     2461 cttgctgaag gccagaaacg tagcgaggag atcaaccgcc tgaatcgcca gttgcgggtg
     2521 gaaaaaattc tgaatcgctc aggcatccag ccgttgcacc gtaaatgctc gtttgcgaat
     2581 taccaggtgc agaacgacgg ccagcgatac gcgttaagcc aggcgaaatc catcgccgat
     2641 gaactgatga ccgggtgtac aaattttgcg ttcagcggaa aacttggtac cgggaagaat
     2701 cacttagcgg cagctatcgg gaatcgcctg ctgaaagacg gtcagacagt gattgtggtt
     2761 accgtggctg atgttatgag cgccctgcac gccagctatg acgacgggca gtcaggcgaa
     2821 aaatttttgc gggagctgtg cgaagtggat ctgctggttc ttgatgaaat tggcattcag
     2881 cgcgagacga aaaacgagca ggtggtgctg caccagattg ttgatcgccg gacagcgtcg
     2941 atgcgcagcg tggggatgct gacaaacctg aactatgagg tgatgaaaac attgctcggc
     3001 gagcgggtga tggatcgcat ggtcatgaac ggcgggcgct gggtgaattt taactgggag
     3061 agctggcgtc cgaatgttag ccattcgagg gttgttaagt agtttcagga ggatttatgg
     3121 cgaaaccttt tactcccgaa cagcgggaag aactgaagac gcgaattgtg gaactcgtgc
     3181 atcaggacgg tcgggtcacg attcggcagt tgtcagatga aacaggtatc agtcgtgcgt
     3241 ctgtcggtcg cttatgcata gaactggtcg caagtggtga tgtatataat tctggctacg
     3301 gcttattccc gtctgaacag gctcgcaagg actggcaaag cgcccgcaaa aaactctcga
     3361 gagtaaaggt gaggaaaccg gttgttgttg atccggacct tatctggtca ttacctgacg
     3421 gagaaatacg ccgctacgac aggcgcctga atataatctg tcgcgagtgc cggaagagcg
     3481 aagctatgca gcgtgtactg gctttctatc agggtaattt tcaggaggcg atactgtgaa
     3541 tgaaattagc tatcaggctt caattaccgc tggcattcgc atcaaaggag aggagcatgg
     3601 aaataaaacc agaagatgag ttaagcaata tcgttttatt tccggtaaaa gaggatgacc
     3661 ctcgtaatca ggttaatttt ctttatgagc catcggaaag accatattgc catcacgcct
     3721 ctgtacgggt tgacgaaaaa gagcgtcagg tccgctgtaa aatctgcggt gcggttgtgg
     3781 agccgtttga ctggatgctc tctgtggcaa aaagagaaac cagactggca gatgatgtaa
     3841 ggctattgcg ccaggaggaa caggaaaggc ggagaaatat agaaaagctg atacagattg
     3901 agcgtaacgc gaaagcgcgg atacgcaggg cgacaaaatc cagaactgaa taattaaatt
     3961 tagcactgtt aaaaatttaa tccttaaccg gagggatttc tgcaccctca aatcatcagg
     4021 agaccacccg aaagggcggg gagcagtcac acatctgttt ccgatagccc cgttctaatg
     4081 ctacactctt tgatattttt atgaccccaa taaacatatt tatgacagtt gctgatttca
     4141 aacggcccaa attggagctc ccaaacgggg caaacaaact actactgcac tcttgctgtg
     4201 ctccatgttc cggtgaggtg atggaggcgc ttcaggcctc gggaatcgac tacaccatct
     4261 ttttctacaa cccgaacatt catcctcaga aagagtattt aattcgtaag gatgagaata
     4321 ttcgctttgc tgaacaacac ggcgtgccgt ttattgatgc tgattacgac acagacaact
     4381 ggtttgaacg agccaaagga atggaatggg agcccgaacg agggatccgt tgcaccatgt
     4441 gttttgacat gcgttttgag cggacagcgc tgtacgccgc tgaaaatggt ttcagtgtga
     4501 tcagcagttc actgggcatt tcacgctgga aaaatatgca gcaggttaac gactgtgggc
     4561 ggcgagccgt cgcgcattat ccgggcatgg tgtactggga ttataactgg cgcaagcagg
     4621 gcggctcgtc ccgtatgatt gaaatcagca agcgcgaaaa attctatcag caggaatatt
     4681 gtggctgtgt gtattctctg cgcgatacca atctacaccg caaatctcag ggacgccctc
     4741 ttatcaaaat tggtcaactc cactacggta aagaagagaa ggagtgattt tatggggcac
     4801 ctttctgatt gatttcatat tggcgaggta agtagaatga ctgcgggtgc ttgaggctat
     4861 ctgcttcagg catgaacacc aaaaggcaga tagagaaaag ccccagttaa cattacgcgt
     4921 ccggcaagac gcttaacatt aatctgaggc caatttcatg ctttgcacat gtaggttagc
     4981 ctcttacatg ccgaaaggca aggagaagca ggctatgaag cagcaaaagg cgatgttaat
     5041 cgccctgatc gtcatctgtt taaccgtcat agtgacggca ctggtaacga ggaaagacct
     5101 ctgtgaggta cgaatccgaa ccggccagac ggaggtcgct gtcttcgtag actacgaatc
     5161 caggaagtaa gagtgaccgg gcggggagct gatcccatcc ccgcccacct ctgatgtgtc
     5221 aggcatcctc aacgcacccg cacttaaccc gcccatcgct gtgatctctc agcgtttcgg
     5281 cgggtttttt gttgtttatt tccggtgaat ttgattcgcg caccttcgca gatagaatcg
     5341 actcacttaa gtagcgcgca gggagaagag ggatggaccc cgaacagggg agagctattt
     5401 atctggaagg attctgaaga tgaaaatcga agaattgcgt gaaattttta gtgaaaatgg
     5461 cctctatgct gtgcgcgttg agaatggagc gattgtcagc cattgccgca ttagatgttt
     5521 gcaatctcaa caaaggaaga gcggtgctgt gttattttat ttttgtaatg gacttctgac
     5581 ggacggtttt attttgcgtg aggacgaatt tgtcacatca ttacgggttt tgaaagagat
     5641 tggttttaag gctggttttc tgcttttgct gaagaataaa ctcatctaca atcttgagca
     5701 ggattgaact cctgctgtgt aacaccgtgc caccggagaa agccgatggc acatatacaa
     5761 ctggtcaaac aaacctcttc cggattactt ctcccggcga cgccggagag ttgcgatttt
     5821 ctgcatcaaa tcaaaatagg tgagtggata cacgcagact ttaagcgtgt gtgtaactac
     5881 gcattccaca agcgtttttt caaactcctg caactgggtt tcgattactg gactccggtc
     5941 ggtggggcga tcacgcctcg cgaacgaaaa ctggtgtccg gtttcgttga ttacctgtgt
     6001 gaatcagtag gccgggaaca tacgccagct ctgagcgaag ccgcagagca atatctgaat
     6061 acagttgcga cacgcagaac cagtgatacg gcattgctaa agtcgtttga ggctttccgc
     6121 gagtgggtag ccattcaggc cggattttac accgagcatt tttatccgga cggtagccgt
     6181 gggtgtcggg cgaaatctat cgcgtttgcg aatatggacg aaaccgagtt tcagcaggtt
     6241 tataaatctg ttctgaatgt gctgtggaac tggattctgt tccgtaaatt ttcctctccg
     6301 gaacaagtcg aaaatgtggc cgcgcagctg ctggagtttg cgtaatggtg gatttacgta
     6361 aagcggcgcg ggggcagatg tgcaccgtca gaattcctgg ctactgcaat cacgatccgg
     6421 aaacgtctgt gctggcgcat taccgactgg cgggaacgtg cggaacagcg ataaagccac
     6481 acgatatgca ggcagcgatt gcctgtagct cgtgccacga tttaatcgac gggcgggtaa
     6541 aaaccagcga ttacaccaaa gaagaattac gcctgatgca tgcagaaggt gtttttcgca
     6601 cacaagaaat ctggagaaag gagggatatt tatgatttac ccaacgaata caggaaaaag
     6661 cggagaacac cttcgtctca ccacgctgga aagtgtctgg attcagggaa aactacgtat
     6721 gtgggggcgc tggtcgtata ttggtggcgg taagacgggg aatatgttta acctgatgtt
     6781 gacctctaaa aagctgacaa aaacggcaat taacgaggcg ctccggagga tgaaaaaagc
     6841 aggtctgaac aagtctgaac ttgaggcttt tttgcgggat atgattaacg gtaagcaaaa
     6901 gagctggctg gcgcattgta ctgatgcaga ggcgttatgt attgatcggg tcataagtga
     6961 ggtgctggca gagcatccag gattgattag cgtccttcgg caacggtatg aggggcgggg
     7021 gatgaccaaa cgcaaaatgg ctgaactgct gaatgatgca cacccgaaat ggagtttaag
     7081 aacctgtgaa agacgcattg agcattggct aaaggtggca gaatttattt tgtacaaacc
     7141 aatggttatg gcttttggta tagagaaaaa agttattgct ttttgacgta aaaactgctt
     7201 caattcttgt acgcttcgca aagctgtacc gcgaggcgaa tagcagacat ggacatttga
     7261 aagagcccgc ttta
//
LOCUS       DAESHI010000172         6967 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.174, whole genome shotgun sequence.
ACCESSION   DAESHI010000172 DAESHI010000000
VERSION     DAESHI010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6967
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.174"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(416..901)
                     /gene="sixA"
                     /locus_tag="KW011_003989"
     CDS             complement(416..901)
                     /gene="sixA"
                     /locus_tag="KW011_003989"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HBH7662205.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(1104..3248)
                     /gene="fadJ"
                     /locus_tag="KW011_003990"
     CDS             complement(1104..3248)
                     /gene="fadJ"
                     /locus_tag="KW011_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HBH7662206.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3248..4558)
                     /gene="fadI"
                     /locus_tag="KW011_003991"
     CDS             complement(3248..4558)
                     /gene="fadI"
                     /locus_tag="KW011_003991"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HBH7662207.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4738..5022)
                     /locus_tag="KW011_003992"
     CDS             complement(4738..5022)
                     /locus_tag="KW011_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HBH7662208.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5394..6734
                     /gene="fadL"
                     /locus_tag="KW011_003993"
     CDS             5394..6734
                     /gene="fadL"
                     /locus_tag="KW011_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HBH7662209.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYISWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1767 a         1845 c         1637 g         1718 t
ORIGIN      
        1 taagtattta gaaaaaatag aaagtatatc aataatgaat atcaacagct taaaaaacaa
       61 acaatgaaca tataatgcga ttggcattta tccacttttg acaatataat atataaacac
      121 tatagccaga atttaatcaa gtttcaatta catcctgcct gattattcaa ctctattttc
      181 tctattatct gtattaataa atgacaatga gtgtcaaaca gacattattc atttcaacat
      241 gtaactttgg gcaaaatcaa gatacgcgca gaagaataat ttccgaatga aagtagaaag
      301 tgttttattt atagatgggg agagtcttcg atagtagtga gattgacaga tatccgctgc
      361 ctgatgagca attatgccca atgaatctac ctcattgggc ataatttgga actcatcaga
      421 tagcttttgc catcttcaga ttacacgggc tcatctgcca gttgaatgtg ccgtttccgc
      481 tttcatcaag cgttacgctg gcaatggccg aggtggtaaa cataggcggc gtttcgcccg
      541 gacataactc ggcaaccaga tacccgacta acggtaagtg agagatcacc agtaccgaag
      601 caacaccttc attggtcaac gcctgcaaat aggcactgac cagaccaaca tcgccgcagg
      661 gcgttaactc cggcagaact tctgcactgg aaggcaggtt cagacaatcc cctacttctt
      721 ccagtgtttg ctcggctcgc aggaacgggc tcaccagaac gcgttcgatt tccacttttt
      781 gacctttcag ccagttcgcc atcaggcgag attcgtcaca accattagtg gtcagaggac
      841 gaaccgaatc actggcggca tcaagggctg cgtcgccgtg acgcatgata aaaacttgca
      901 tattgcaccg cttttgttaa ccagattcgt ccgttttctt tcatctgaaa ccagagaaag
      961 aaaacggtgg ccggcattgt gccttatcca ttcaccgaat gaaacgctgt tttttacctc
     1021 aatggcgtaa gtatagtcaa tatgcgttta cattttgacc aacactccgc cattcagcgc
     1081 ggattcatat agctttgacc ttcttattga aggtcagttg cagttgtttt ccaaaaactt
     1141 tccccacgcg cgcccatctc gaccaaacgc tcgcaagggg taaaacggga accatactgc
     1201 gtggccagtc gttgcattat tgcaaccact tcgcccgcgc cgagagaatc gatatagcgg
     1261 aacggtccac cgagaaatgg cggaaaacca atgccaaata ccgcgccaat atccccgtca
     1321 cgcacgctac ggataacctg ctcatcaaca caacgtactg cttcattcag catcaacatc
     1381 acacaccgtt cagcaacctg cggtgcggag agtcgcccct gcccttgtgc gccaatgagc
     1441 gggtaaatgg cgggatcgac ctgttttttg cttttacgcc ctttctgacc ataaagatag
     1501 aaaccccgac cattttttct gcctttgcga tcgtcgttca aaattgaaga aacaacattt
     1561 gcaggcgcgc taaaacgttc tccataagcg gcttccagta caggaataat tttagtcccg
     1621 gtgtcgattc ctacctcatc caaaagttgg attgggccta ccggaaaacc aaatttcact
     1681 agcgcggcat caatgtgctc tacccgttca ccttcggtca gcatgcggat agcttcatta
     1741 atgtaaggcg ctaagatgcg attgacgtaa aacccggctt tgtcacgcac gacaatgggc
     1801 gttttgccct gttttttcgc cagttttacc gtagtggcga tggtttgcgc cgatgtaccc
     1861 gcatgaggaa taatctctac cagcggcatt ttttccacag gactgaagaa atgcaggcca
     1921 ataacttgct caggtcgcgc ggcgtgagcg gcgatatcac caatcggtaa agatgacgtg
     1981 ttcgaagcaa aaatggtatg agcggcgcaa ttttgctcca cttccgccac catctgttgt
     2041 tttaattcga gattttcaaa caccgcttca ataatcagat cgcgatgggc aaagccgcga
     2101 tagtccgtcg ttccggagat taatgccaac tgtttgtcac gttcgctggc tttgagatga
     2161 cgacggcgaa ctttgccctc cagctgatcc caactgtact tcagcgcatg atttatgccc
     2221 tgcgggttga tatctttaat tctgaccgga agccccgctt tacaagcagt gacataagca
     2281 ataccgccgc ccatcaagcc accacctaaa attcccacgc tgtttaatgg cgcaggcggt
     2341 gcatcactac cgggatcttt cttcacgtcc gtactggcaa aaaagatact acgcaaagct
     2401 tgcgattgcg gcgtcatcgc cagttcgcca aaggcgcgag cttcagcgtc atagccgctg
     2461 ctggtgccct gcgctaatcc cgtttcaaca acctccagga tgcgttctgt cgccggataa
     2521 ttgccttgag ttttgtgttc tgttttcttg ccgaccattt tgaacagcag cgcacgacct
     2581 aacggccccg ccagaatacg ctcgcgtaca ggtagagggc gggaagatgg gcgatccttc
     2641 ttcgccagct caacagcggc ttccagcaaa atggagtgcg gaacaacgtc atccaccagc
     2701 cccagcttta atgcctgttt cgcccgaagt tgttttccgg tgagaatcat ctctaatgct
     2761 gtactgacgc ctatcagacg cggtaaacgc tgggtgccgc ctgaaccggg taacaatcca
     2821 agttgtactt caggcaaacc gagcaccgtt ttaggatcgt cagtacaaac gcgaccgtgg
     2881 cacgccagcg ccagctccag cccaccgccc aggcaagcgc catgaatagc cgcgataacc
     2941 ggaatgggca aagcatgaat ctccgccatc aactgttggc cctgccgcgc cagtgcttcc
     3001 gcttcttgcg ccgttttgca gttgccaatc atgttgatgt ctgctccggc gataaaattg
     3061 tccggtttcg cggagataaa caccacgccg cgcaactctt tgttttcacg gagttgctta
     3121 ataatggcgc gcacctgcga ggcaaattcc gccttcaggg tattcatttt ctcatccggt
     3181 acatcgatgg tgatgatagc aatgttgtcc agacgaacat taagagtaaa cgctgatgcc
     3241 atttccatta ttccgcctcc agaaccattg ccgcgccaag cccaccggca gcacaggcgg
     3301 taactaaacc aaatccaccg ccgcgacggc gaagttcatg caatgtctgg gtaatcatcc
     3361 gcgcgccggt cgccgcgaag ggatgcccgt aagcaatcga accgccaagc acgttaaatt
     3421 tgctatcgtc cacttcgcca gtggcatgtg cacgccccag tacttcacga gcaaaacgtt
     3481 cactacccag caactgaata ttcgccagtg tctgagctgc aaaggcttcg tgcatatcga
     3541 tcaatgtcag ttcgctcatc gtcaaaccgg cacgttccag cgccagcggt gttgaccagg
     3601 ctggaccgag caacatgtcc tgccagacat caatcgcagt aaatgcgtag ctgcgcagat
     3661 accccagcgg caccagccct aattctttcg cccgggattc agtcatcagg atcaccgctg
     3721 ccgcgccatc ggtcagcggc gtactgtttg ccgccgttac cgttccgtgt ttgcgatcaa
     3781 acgccgggcg cagctttgcg taatcggcaa gcgaggaatt accgcgaata ttgttgtctt
     3841 cgacaagcgg ttgtttataa ggagggataa aggcagtcat cacctcttct ttgagttttc
     3901 cgtctgacca tgcctgagcg gcacgctgat gcgaacggtg cgctaatgca tcttgctgtt
     3961 ctcgggtgat gccgtaggtt ttcgccattt gttcggcggt gtcgcccatc cgcaagccag
     4021 tagaatattc tgctaccgca ggcggtacgg gcattaagtc gcgcaaacgc aggcgagaga
     4081 agagtttcag gcgctggctc atggttcgag ctttgttgac atcaaccagc acgcgcgcca
     4141 gttttttact gacgccaatt ggcaatacgg aagaggaatc tgccccaccg gcaatccccg
     4201 ctcggatagt tcccgccatc aggctttctg cgacgtttgc aaccgcctgg aaactggtag
     4261 cgcaagcgcg gctgacgctg taagcatcgg tatgcacatt cattccggta ccgagaacaa
     4321 tttcacgcgc aatgttgggg gcttcaggca tttgtacgac ctgaccaaag accagctgtt
     4381 caatcacttc agcggggatc tcgctgcgtg ccagcagttc gcctaccacc atcttcccta
     4441 aatcaaccgc gggaatgcca tgaaaagccg tcgcctggcg ggcaaaaggc gtacgtaaac
     4501 cgctaacaat ggcgatacga tcgccctggc gggtaaccag cggtaaaacc tgacccataa
     4561 cactcccctg taaaaaaata aataaagtgg tctgacctga tcatagtctt aactattttt
     4621 ttacatttag ccaagcggag aaaaaggaaa gtgggagcta tgacacagtg aagacaggag
     4681 aaagaaaaga aaacgcccct gccagcaact gacaggggcg aacgtacccg agaggaatta
     4741 acgcagaccc agctggaaga tcagcatttc agcttcgcag gcaaaagtga aatcgatatc
     4801 caggcgcaca ccgtcagact cttcagtgaa agtcggggag attttgcacg gttcggattc
     4861 cacgctacgg gctttttcag tcagcgccgc cagcgtttgt tctgcttctg cgcggtttgc
     4921 aaacacgcgg ctgtaagacg cggtgcagtc ggagttgtcc ataatggtgc caacatccat
     4981 acagcagcaa accggggttt catcagcact acatttactc atcgttgatt tcctctgtat
     5041 gtgcacccaa ggtgccagat aaacgttgtg gatattttac gcttccggaa agtgctgctc
     5101 cagttgttaa ttctgcaaaa tcggataagt gaccgaaatc acacttaaaa atgatctaaa
     5161 acaaaattca cccgaatccg cgagtgcgca acctccaaat tttgccagct ggatcgcgtt
     5221 tcttagatca tatttgaaaa aagatggaaa catacttgca gcattccagc tggtccgacc
     5281 tatactctcg ccactggtct gatttctaag atgtacctca gaccctacac ttcgcgctcc
     5341 tgttacagca cgtaacatag tttgtataaa aataaatcat tgaggttatg gtcatgagcc
     5401 agaaaaccct gtttacaaag tctgctctcg cagtcgcagt ggcacttatc tccacccagg
     5461 cctggtcggc aggctttcag ttaaacgaat tttcttcctc tggcctgggc cgggcttatt
     5521 caggggaagg cgcaattgcc gatgatgcag gtaacgttag ccgtaacccc gcattgatta
     5581 ccatgtttga ccgcccgaca ttttctgcgg gtgcggttta tattgacccg gatgtgaata
     5641 tcagcggaac gtctccgtct ggtcgtagcc tgaaagccga taacatcgcg cctacggcat
     5701 gggttccgaa catgcacttt gttgcaccga ttaacgacca atttggttgg ggcgcttcta
     5761 ttacctctaa ctatggtctg gcaacagagt ttaatgatac ttatgcaggc ggctctgtcg
     5821 ggggtacaac tgaccttgaa accatgaacc tgaacttaag cggtgcgtat cgcttaaata
     5881 atgcatggag ctttggtctt ggtttcaacg ccgtctacgc tcgcgcgaaa attgaacgtt
     5941 tcgcaggcga tctggggcag ttggttgctg gtcagattat gcaatctcct gccgggcaaa
     6001 ctcctcaagg gcaagcattg gcagctaccg ccaacggtat cgacagtaat accaaaatcg
     6061 ctcatctgaa cggtaaccag tggggctttg gctggaacgc cggaatcctg tatgaactgg
     6121 ataaaaataa ccgctatgca ctgacctacc gttctgaagt gaaaattgac ttcaaaggta
     6181 actacagcag cgatcttaat cgtgcgttta ataactacgg tttgccaatt cctaccgcga
     6241 caggtggcgc aacgcaatcg ggttatctga cgctgaacct gcctgaaatg tgggaagtgt
     6301 caggttataa ccgtgttgat ccacagtggg cgattcacta tagcctggct tacatcagct
     6361 ggagtcagtt ccagcagctg aaagcgacct caaccagtgg cgacacgctg ttccagaaac
     6421 atgaaggctt taaagatgct taccgcatcg cgttgggtac cacttattac tacgatgata
     6481 actggacctt ccgtaccggt atcgcctttg atgacagccc agttccggct cagaatcgtt
     6541 ctatctccat tccggaccag gaccgtttct ggctgagtgc aggtacgact tacgcattta
     6601 ataaagatgc ttcagtcgac gttggtgttt cttatatgca cggtcagagc gtgaaaatta
     6661 acgaaggccc ataccagttc gagtctgaag gtaaagcctg gctgttcggt actaacttta
     6721 actacgcgtt ctgataacgc gttcgcctgg ataaagtcac ctgcatagca ggtgacttta
     6781 actcccccac ttcaccgaaa gtagtgtcct cattgcttac ctcctgagtt ttgcaaacag
     6841 cctgttggca gcttgccctt tcaaatcaat aagcggtatc cacagacaaa cctgaaggga
     6901 aaggcatatt ttcaggcgtt ctgctcgtcc ttctcaaaga gtttactttt ctgcatttcc
     6961 aggatac
//
LOCUS       DAESHI010000173         6826 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.175, whole genome shotgun sequence.
ACCESSION   DAESHI010000173 DAESHI010000000
VERSION     DAESHI010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.175"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(155..667)
                     /locus_tag="KW011_003994"
     CDS             complement(155..667)
                     /locus_tag="KW011_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HBH7662210.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            862..1626
                     /locus_tag="KW011_003995"
     CDS             862..1626
                     /locus_tag="KW011_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662211.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1577..2980
                     /locus_tag="KW011_003996"
     CDS             1577..2980
                     /locus_tag="KW011_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBH7662212.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            2980..4446
                     /locus_tag="KW011_003997"
                     /pseudo
     CDS             2980..4446
                     /locus_tag="KW011_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            4337..5080
                     /locus_tag="KW011_003998"
     CDS             4337..5080
                     /locus_tag="KW011_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBH7662213.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5084..6304
                     /locus_tag="KW011_003999"
     CDS             5084..6304
                     /locus_tag="KW011_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HBH7662214.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            6308..6814
                     /locus_tag="KW011_004000"
     CDS             6308..6814
                     /locus_tag="KW011_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662215.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
BASE COUNT         1817 a         1621 c         1868 g         1520 t
ORIGIN      
        1 atcagcgacc agacagcatt gagaatgctt caggagtaca tcaggatcca atgccttggg
       61 gggtagcggt aattttactc attatccttc aaatcagatt ctgttgtcag aggaatgggg
      121 gaagctggat tcgcaagagc tttttatggg cagtttagct aaaaaaaggc aacacgaagc
      181 taaaactacc aacataaagt gctgaccagc tagcagctga aatcagactc actgtataca
      241 ctttcactgg atgaagttgc aacattcctg ccaccaaagg agcaatataa cgcagcacgg
      301 cgataaagcg cgaagtgaaa agtattgaca cagagttatt ttgcaactga aggcgaaccc
      361 gttctattgt attggaacgg cttgatatta tacatgcaag ttgagggata tgcccaatta
      421 ttataccaag atggtaatta acgatagtac cgcaccatgc gccactcatg accgtgattc
      481 cagcttccca tggtgaaaga gtcgtcttgc tgactgtaat tacagtggcc atcataattg
      541 atgctggtgg caatatcgcc gaaatgagta aagtggattt tgtgaacgct attataaaaa
      601 gaagccccca taggcgatat ggatgaagta caaaatagtt catcaaagca tttatccact
      661 ccatcaatgt ctttccttct gtgaattttt ctttgctatt taaccgtgat acgacttaat
      721 ttaggcttaa acaccttaca tttacaaggt actcctgtgg ggggccctgc cacggagcgt
      781 cgcaggagtg agtgatgagg aagaattaaa gcaacctatt gtgacaggca gcgataacag
      841 taaatcacag gaagcataat catggcaaaa ccggactggg aggccatcga atcggcatac
      901 cgggccggag tccttagcct ccgtgatata ggcgagaaat acggcgttac agaaggggct
      961 atcaggaaga gggccaaaaa gcttggttgg gcacgcagtg gcggtacgca ggtttgcaaa
     1021 aatggtacgc aaaaaaggaa agtgcgtacc agcagaaagc ctgccattac tggccttaca
     1081 caaaaaagta cgcaactaaa aacagaatct acaccggata cgaaaccgat acgcggaatg
     1141 cgtaccgatc ccccgactaa cccattccaa cccggtaacc agcaggcatt aaaacacggt
     1201 ggttatgccc gtcgccttct gctcaaagat gaggtgatag aggacgctaa agcgttgacg
     1261 ctcgaggacg aattatttcg ccttcgtgct aacaaccttg tagctgcaga gaatattggt
     1321 cggtggttgg tgtcgctgga agatgctaat ggggaccagg aaaggaagat gctgattgaa
     1381 aatatcagcg ccgccgagaa agcaatgatg cgcaatacag ttcgtattga gtccatcgtt
     1441 ggcacgcttg cgacggtagg aaaaatattt gctgatacag cctaccgcaa ggccgccact
     1501 gataaggtgt ctctggaggc tgatcgtctt cgccgtgatg caggtattga tgatggcaat
     1561 ggagagcgtg acctcaatga cttctactct gacatccaaa ccgacgctga atccggcttt
     1621 acgtagtttc tggactatgc gggcacgtaa caaagtgctt tatggtggtc ggtcatcgtc
     1681 aaaatcatgg gatgccgctg gcattgccat atttctgtcg aataaataca ccctgcgttt
     1741 ttgttgtgcc cgtcagatcc agaataaaat cgaagagtcg gtgtataccc tgctcaaaat
     1801 tcagatagac aggtttggtc tgcggcaccg tttccgtatt ctgaacaaca aaatcattaa
     1861 ccgggttact ggctcggaat ttgtttttta tggattatgg cgcaacatcg aagaaattaa
     1921 gtcactggag gggatcgatg tgttgtggct ggaagaagcc cacgcactga cggaatacca
     1981 gtggaaaatt ctggagccaa cgatccgtaa agagggttcg gaatgctggt tcatattcaa
     2041 ccccggactt gttactgatt tcgtctggcg caacttcgtt gttgatccgc ccgaaggcac
     2101 tctcatccgc aaaattaact atgacgaaaa tccgtttctg tctgacacca tgcttaaggt
     2161 tatcgacgcg gcgcgacgcc gtgatccgga tggttttaaa catgtgtatg agggcgttcc
     2221 ggagtctgat gatgatgcgg caatcatcaa actgtcttgg atagaagccg cagtggatgc
     2281 gcacaaaacg ttaaatttcg aacccagtgg aagaaagcgt attggctttg acgtggctga
     2341 cagtggtaca gataagtgcg ctaacgttta ccgtcacgga tccgttgttt tctgggccga
     2401 cgaatggaag gccaaagaag atgaattact gaagagctgc cagcgtactt atcaggcggc
     2461 gctggagcgt gaagcagata ttgtttacga ctctatcggt gttggtgcgt ctgccggtgc
     2521 taaattctct gaaattaacg ctgaccggaa gagcgagaac gcatacgcgc gacgtgtgaa
     2581 ttaccagagg tttaacgccg gtgctggtgt gcatgagcca gatgacgagt acaacggcat
     2641 ccccaacaaa gactttttcg caaatcttaa ggctcaggca tggtggctgg tggctgaccg
     2701 tttcagaaat acgtttaacg ccattaacaa cggagaacag tatcctgtgg atgagctgat
     2761 cagcatagat tctcgttgtc cgttgcttga aaagctgaaa ctggaactga caacacctca
     2821 tcgtgatttc gaccgtaacg gacgtgtgat ggtcgaaagt aaaaaagacc tcgcaaaacg
     2881 cgagataccg tcaccaaacg ttgctgatgc attcattatg gccttcgcgc caatcgatac
     2941 atcgctggat atctgggaac agctggggag acaggcctga tggcacgaaa caaacaagcc
     3001 ctgcggcgaa ctgtgcaggc cacagctgat ggttatgaga attttattgc ccgcgtaggg
     3061 atgcagacac ctaaccagca ctcagcatcc acctaccggg ctaatttcac cagtcgtaac
     3121 cgcatgctgg tggaatggtc ctatcgttca tcctagatca tcggcgaagc agtcgatgct
     3181 atcccggatg atatgacccg caaaggcatt cgcatcactt cggaaattga tgcaaaagat
     3241 cgtggcattc tcgaatcaca actggatgag ttgcaaatct gggatgcgct gaatgacgtg
     3301 ctgaaatggt cgcgcctcta cggcggcgcg gtgggtttca tcatgattga ggggtaggca
     3361 ccaatgaccc cgctgcgacc cgaaaccatc ggtaagggca agtttaaggg gattctcccg
     3421 ctcgaccgct ggatggtcga cccggcactg acccgccgca ttaaagatat ggggccggac
     3481 ctgggtaaac ctgagtttta cgatgtggtg accacagcaa cgggaattcc tgcctggcgc
     3541 attcatcaca gtcgcctgat tcgctttgat ggcgtcacgt tgccatttca gcagaagatg
     3601 accgagaacg aatggggaat gtcggttgta gagcgtatct gggatcgtct taccgcgttc
     3661 gacagcgcta ctgtcggcgc ggcgcagctg gtctacaagg cgcatttgcg tacctacagc
     3721 gtggagaagc tacgcgagct tatcgcactt ggtggtcctg cgtatgaagc gttgctgaag
     3781 aatatcgacc tgattcgaca gttccagagt aatgaaggca tgacgctcat ggactcgcgg
     3841 gataagtttg aaacccatca gtacagcttc agtggtctgg atgacatcct ttcgcagttt
     3901 gtagaacaga ttagtggcgc tgttggtatc ccactggtgc ggttgttcgg acagtccccg
     3961 aaaggatttt ctaccggtga tgcagacctt gccaactatt acgatcgcat cagttcgttg
     4021 caggagaggc gtttacgtct tccggtgcgg cggatactgg acatcatgca tcgttcggaa
     4081 cttggcaagc cgctgccgga cgatttcacg tttgagttta acccgctctg gcaaatgtct
     4141 gatgtcgatc gctcaacggt ggcgttaaac accaccaacg caatcagtac ggcgctgggt
     4201 gatggtctga tgacactgaa agccgctatg actgatttgc gagaaaattc tgacgtaacc
     4261 ggcatcgggg catccattac cgacgaggac atcgagaatg ccgaagatga agcgccgccc
     4321 ggcatcggcg aacctgatga cgaaccgcag gagccgtcag gcggaaatcc ggtatcgaac
     4381 cagcctacgc aggatagcga gggcggtcgg agacatcgta aatggtcgct acgatggttc
     4441 aaatgacagt atcacggaaa ttattgcggc gctggagcgt tacagtgaaa tcatcacccc
     4501 ctgggcgaca aaggtcgcgg aaaactttac tgcggaccta acccggcaga acgagaaagt
     4561 ttggcggcaa cacagcaaga acatcagtcg cgagctccgc aatcttgtgg aaagcgctcc
     4621 tgtgggccag gtgatgcaat ccatcatcgc cgaacaggtc aagtacatca aatctctgcc
     4681 tctcgaggcc gcagacaggg tgtacgacat ccagaacaaa gcgatagagg ctgttgtcac
     4741 cggtggtcgg gcggagcagt ttgctaaaga gattgcatct accggcgatg ttgctaagtc
     4801 tagggccgat ctgattgccc gaacggaact gggaagagca acgggcgcgc tggatatggc
     4861 ccgagcgata gctattggct cggatggtta tatttggcgt acagccgatg atggcgatgt
     4921 ccgagattcc cacgatcaca tgaaaggtaa attcgtccgc tgggattcac ctccaacttt
     4981 ggacggcatg accggccacg cgggcgagct gccaaactgc cgctgctata aagagatcgt
     5041 gtttgttcgc gttccattcg caatgaaaag ggcagcataa cccatgaaat acttttttga
     5101 gaccaggctc ggggaaaccc gataccgcct ggctgacggc tcgttgctgt gcaaagacgt
     5161 gccgatagga cgaacaggta agcagctcta tggtgctgat gacctgccaa aactgaaacc
     5221 cgataagttc ggtgaaatag tcgtcacgcg ttctcctgag caggtattcc atccggccac
     5281 gcttgcctca ttcgaaggga tgagcatcac gatcctgcat cctgaagatg aaaacgggaa
     5341 tgtgcggctg gtaaatcccg agaactggaa agagcttgct gtcgggcacc tccagaatgt
     5401 ccggcgcggg acgggtgagc agtctgattt gatgctggct gaccttatcg tcaaagacga
     5461 aagcgccatt cagcttatcg aagatggcct gcgcgaagtg tcgtgcggct atgacgcgga
     5521 gtacgagcag accgagccag gtaaagccga gcaggtcgat attaccggaa accatgtggc
     5581 tcttgtccct aaaggcagag ccggaaatcg ttgtgcaatt ggagacagag acacaatggc
     5641 aaatcaaaag aaaaactggt ggaaccgcat gcgtgcggcc atcaagacag gagatgccga
     5701 caccatgaac gaactggtgg agtcggctcc cgcatcggtt acaggagatg agggggattt
     5761 gccgcagggc gttaatctca acatcaacct gtccccgcag caaccactac cggacaaagc
     5821 accagagatg ggcggagatc caaccggcga cagtgatgat gacctcaaaa cattactgaa
     5881 agccctgctg gctaagctgg aaagaaatgc cacgggcgat aacgataata agcctgacga
     5941 taatccgacc ggtgacggcg aggacgatga agaggaaacc acgattactg gtgactcagc
     6001 ctggcgtgcc gaagttatcg ttccgggtat cgatctgagc cgtaagatga aaccgaccgc
     6061 gttcaaacgc gaggttctgg cttctgctga caaaacgctg gttcgccaga tagtcggtga
     6121 tgcggatatc cgcaaattac cgaaacaatc ggtcgacatg gcgtttaatg ccgtgtctga
     6181 ggttgccaaa gggcgaaaca cccgcgccac caccggcgat gcacagcgcc taaacatggg
     6241 catgaccagt atcgcttccc tgaacaaaca aaacgctgaa ttctgggcaa accgtaaagg
     6301 gtaaaaaatg aataatgtat ttctgtaccg gatgcctgtt ggcattgccg gggctgtctc
     6361 tcgcccgcag gacttaaccg tcgaaccggt ggtccttaaa tccgataacg ccttcgctgc
     6421 ctatggcctg gctggtaaat acgatgatga cggttttttc gtgccgctgg cagatggtga
     6481 taccgcagac aaggtgaagg ggatctacgt gcgcccttat ccgaccacgt cgcagccgga
     6541 catggttcgc caggtgggaa caggcaagaa cttcccgggc gacgccatga agcgtggcta
     6601 cgtgaccgtt aatctcggtt ctgattttga tgccagcact atcaaaaaag gcgatccggt
     6661 atacgttgtc gtctccactg atgaatccat caaagtgccg ctgggtggat tcatgtccac
     6721 gtcagtcagt ggcaaaaacg tggtgctgac caacgctgaa ttcacaggtg ccggtgatgc
     6781 tgacggcaat gcagaaattt cctggaagat ttaaggaaca gacgaa
//
LOCUS       DAESHI010000174         6779 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.177, whole genome shotgun sequence.
ACCESSION   DAESHI010000174 DAESHI010000000
VERSION     DAESHI010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.177"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="KW011_004001"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="KW011_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HBH7662216.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="KW011_004002"
     CDS             2089..2238
                     /locus_tag="KW011_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662217.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(2994..3434)
                     /locus_tag="KW011_004003"
     CDS             complement(2994..3434)
                     /locus_tag="KW011_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HBH7662218.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3652..4041
                     /locus_tag="KW011_004004"
     CDS             3652..4041
                     /locus_tag="KW011_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662219.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4085..4489
                     /locus_tag="KW011_004005"
                     /pseudo
     CDS             4085..4489
                     /locus_tag="KW011_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4596..5432)
                     /locus_tag="KW011_004006"
     CDS             complement(4596..5432)
                     /locus_tag="KW011_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HBH7662220.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5432..5707)
                     /locus_tag="KW011_004007"
                     /pseudo
     CDS             complement(5432..5707)
                     /locus_tag="KW011_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            complement(5782..6255)
                     /locus_tag="KW011_004008"
     CDS             complement(5782..6255)
                     /locus_tag="KW011_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HBH7662221.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            complement(6276..6779)
                     /locus_tag="KW011_004009"
                     /pseudo
     CDS             complement(6276..6779)
                     /locus_tag="KW011_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
BASE COUNT         1581 a         1755 c         1771 g         1672 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt ttacatcact tatacatcac ttttacatca ttccttcagc tcatacgtca
     2641 ctttcatcct ctgtgccatt tgtacatcac ccccttcctg aacgtgctga cagcgggttt
     2701 tccggctgcg ccggcaacgc ttcacggcca cgcaataaat tgcgtccccg ttcaccgttt
     2761 tcggatggga agaacgccgg aaacaggact taccccggct gaatggtgtg aatttccgcg
     2821 ctatgcactt gtgcgcatac tcatgcatgc cgtaaaaaca gagcctgcgc gtttctggcg
     2881 ggttttcggg tggtttgttg cctgttttac cggtttcccg tcagaaacgc cctgagggcc
     2941 tctcaggcgg tgctgataac agatgttatg gtaaatcaga gtcacctgca ctgttactct
     3001 gaaccggagc gaaacagccg cgaccagaat ccccgctttt tttcctgtgc cagcgcgtca
     3061 gccagacggc taatttcggc ctgtagttgt tccctctcct catttcgcag gcggttttct
     3121 gcttcccgcg ccatcttgtc ttcaagcatt agggtaacag tctgctgcag agcgtccagt
     3181 ttctgctgaa tgacgtctag ctgagcattt tccgcctggc taacgacaga tttggagtgt
     3241 ccctccttgt gcctttttgg tgtctcagaa gtgcgcagct cgccataggc ccgaatcagt
     3301 tcactggtct cgaactcccg gcgatcattt tttcctgtgc gatagctgac aaggccttta
     3361 gccatgtcac gatataattg cgagcgcgac tttcctgtta atttcagtgt ctcttgaact
     3421 gtgtgccatg ccataaaagt gacagtgtct cctgtgtgta ccgtattgta acagcagaac
     3481 atattactgt tttaacacaa aaggtctgag gctgcccgtg aggcgtgaaa aatacacaaa
     3541 aaacctgttg acggtgtggt tttgacgggc gtagtatttc gaatactgta tataaacaca
     3601 ttgttataaa tacagttttt gctgttttta aaagtcaaag aggataattt tatgagtaca
     3661 gagttaaata gcggtactgt cggtggtttt ggtcacatgg gaccaaatga cccctacaat
     3721 gatggtcata gcggctctgg aagttcggga ggtagtttta acggtggtgg taatggtggc
     3781 catgataata gtagtggttc atcttatact gcatcctttg gtctcatcac tggccgacac
     3841 ccgataactg tcccaggtac gaagaatatt aattctgtcg atctttctgc tgtcattaat
     3901 aacattatac atcatgaagg tgcacgcaat aacatgtacc tagatactgc gaacaatgta
     3961 agagtgggag tgacgggcac tggctggcaa tgtctagcaa cggcaggcat ttcggctgag
     4021 ggtaaaagaa ctttccgcta agcgatagac tgtatgtaaa cacagtattg caaggacgcg
     4081 gaacatgcct catgtggcgg ccaggacggc cagccgggat cgggatactg gtcgttacca
     4141 gagccaccga cccgagcaaa cccttctcta tcagatcgtt gacgagtatt acccggcatt
     4201 cgctgcgctt atggcagagc agggaaagga attgccgggc tatgtgcaac gggaatttga
     4261 agaatttctc caatgcgggc ggctggagca tggctttcta cgggttcgct gcgagtcttg
     4321 ccacgccgag cacctggtcg ctttcagctg taagcgtcgc ggtttctgcc cgagctgtgg
     4381 ggcgcggcgg atggccgaaa gtgccgcctt gctggttgat gaagtactgc ctgaacaacc
     4441 catgcgtcag tgggtgttga gcttcccgtt tcagctgcgt ttcctgtttg gggtcgtttg
     4501 cgggaagggg cggaatccta cgctaaggct ttggccagcg atattctccg gtgagattga
     4561 tgtgttccca ggggatagga gaagtcgctt gatatctagt atgacgtctg tcgcacctgc
     4621 ttgatcgcgg ccgcgatagc tagatcgcgt tgctcctctt ctccatccgc gttccaagct
     4681 gcggaaaggc acccataagc gtacgcctgg tcgagcaggc gacgcggatc gacgtccagc
     4741 gcacgagaga atgcgtccgc catctgtgca atgcgtctag gatcgagaca aaggtcgtct
     4801 ctgtcagccg gatcgtagaa catattggcg gcgccaaagc ccacttcacc gaccagaccg
     4861 acgggatcta tcaccagcca gccgcgactg gagaacatga tgttttcatg atgcagatcg
     4921 ccatgtagcc cacgcagttc cgaggcattg ctcatcattt gatcggctat aatcgccgcg
     4981 tggacgtagt cagtttgaca acctgcgttt tgatcatcgc gcgcccgctg aaacaaagct
     5041 gcaaagcgat cccggatcgg gagaagggca gaaggcaggg gttcctcaga tgcggcatac
     5101 agcttcgcca ttagttccgc tgcaatttcg gtcgcctggt agtcgccgtg ctcggcaacg
     5161 atgtgagaga gcattcgctc cccggcatat tcgagcaaca tcagattgtt ctcacgaccg
     5221 agcaaccgga ctgctcccct cccattgcgc cataccagat agtcggcccc gcgcagttca
     5281 tcagcaatgt cttctatagg tttcaatccc ttgacgattg caggagtccc gtctggcaat
     5341 gaaactttcc aaacgaggct ggaaaaggtg tccgcaatga gaacaggttg cgaaacgtgc
     5401 caatgagcag gaaaaacagg cggcatgaac atcaacccca agtcagaggg tccaatcgca
     5461 gatagaaggc aaggcgttcg cggtcggggg cttcgatccc caatacattg aataggacag
     5521 cgaaggcgcg ctctgcttca tctggcgctg cccagttctc ttcggcgtta gcaatcatga
     5581 gtgccaaatc ggcatagcga tctgctgttc cgagccgccc aaggtcgatc agacccgtgc
     5641 attgaagagt tttagggtcc accatgaagt tcggcatgca gggatcacca tggcaaacaa
     5701 ccatatcgtc gctatcaatc gcggaaaaag gcgtagtgcg cgaagcgaac gaagcttttt
     5761 gccgtcgatt gcatagcttt gttaaccctt tttccaaatt tgatagcaat agttaatgtt
     5821 tgaagtaaag tgttgctcaa aaacaacttc gaaggtattt ggaatactcg ggaagaaaac
     5881 atccccctct ggctcgatgt cgatcgtcga taagtggagc gtagaggcca tgggtaatgt
     5941 ttctcggtaa atttctccgc caccagacac tataacgtga ccggtgaatt cagctagcct
     6001 gtccatggcc tcttcgattg actgaaatac aactacattg tcatcatttg atgtccaacc
     6061 tgagcgggta acgaccgcgt atttcctatt ggggagtgcg cccatagatt caaacgtctt
     6121 gcgacccacc agaagccact gattgtaggt caatgcttta aaaagtagct gctccccttt
     6181 cgcggaccag ggtatgtctg gaccgcaacc aatcacgccg tttttcgctt tcgcagctat
     6241 caatgatact ttcaaggttc tcatcctggg ttaacggtgc gctcttggtc gagccgcacc
     6301 ggtagctctc gttcgacacg agccaaaaga tcgagctgcg gcgtactctt gtcctcgtcc
     6361 ggtaagaagt cgggattgac ggcattgcgg gacaccacat caacggcgcg tccgaacatt
     6421 cgcgacagcc tgcgctcaaa cggacattga tcaaccgata ggctgtgaac agcgccaagt
     6481 tgctgcccca ttgacggcca cgctttgagc aaatccgctc cagacagatc agccgccggt
     6541 actcccggaa ttgccgttat caccaagcat gcaccctcct gttcctcctg ccagttgatg
     6601 acctcggggc aagccacatc tcgacctttg agccaaatga ggcggtcacg ctctccagcg
     6661 agctcaccgc ggcgggaagc aggtgcgatt ttcgcgaagg catgcccgtc accacgtcga
     6721 aaaacaaaat caccagattc tccgcctctg acaggcaacc agtcagaatg cgattcacc
//
LOCUS       DAESHI010000175         6758 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.178, whole genome shotgun sequence.
ACCESSION   DAESHI010000175 DAESHI010000000
VERSION     DAESHI010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.178"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(62..205)
                     /locus_tag="KW011_004010"
     CDS             complement(62..205)
                     /locus_tag="KW011_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HBH7662222.1"
                     /translation="MKKITGIILLLLAVIILSACQANYIRDVQGGTVSPSSTAEVTGL
                     ATQ"
     gene            complement(267..527)
                     /locus_tag="KW011_004011"
     CDS             complement(267..527)
                     /locus_tag="KW011_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBH7662223.1"
                     /translation="MELKHSISDYTEAEFLEFVKKICRAEGATEEDDNKLVREFERLT
                     EHPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKSGFKQG"
     gene            complement(530..2275)
                     /locus_tag="KW011_004012"
     CDS             complement(530..2275)
                     /locus_tag="KW011_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="HBH7662224.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENN
                     PWGGGSGSGIHWGGGSGHGNGGGNSNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG
                     LAVSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLP
                     ADDITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPG
                     VFTASIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPK
                     DSGHNAVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQAN
                     EDVARNQERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFDRFAHDPMAGGHRMWQM
                     AGLKAQRAQTDVNNKQAAFDAAAKEMSDADAALSAAQERRKQKENKEKDAKDKLDKES
                     KRNKPGKATGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRKKFWEEV
                     SKDPDLSKQFKGSNKTNIQKGKAPFARKKDQVGGRERFELHHDKPISQDGGVYDMNNI
                     RVTTPKRHIDIHRGK"
     gene            complement(2508..4727)
                     /locus_tag="KW011_004013"
     CDS             complement(2508..4727)
                     /locus_tag="KW011_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HBH7662225.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTR
                     KRGVDDAVILTPANAPSWCSDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAR
                     EAVLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNERAL
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETE
                     RGEQNRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASA
                     DTLREFIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKA
                     RPVASLLSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFET
                     QWNTWIAPGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETAL
                     VGVITELGRAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGA
                     VMVSDIREGMPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNF
                     YFSDDRRPVASPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVG
                     FVRREGGFCPVLVLPSPDYSHLIVSTITENGVCLAGYGEAINRDEDTPYPPEPHLMQF
                     RLKGHHDRLLAAVHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
     gene            4906..5181
                     /locus_tag="KW011_004014"
     CDS             4906..5181
                     /locus_tag="KW011_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HBH7662226.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRR
                     PEEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEMLPRPPAKPQNH"
     gene            complement(5397..6290)
                     /locus_tag="KW011_004015"
     CDS             complement(5397..6290)
                     /locus_tag="KW011_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBH7662227.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFAYGVRILPKNIAILARFIQQNQ
                     PHALYWLPFDVDRMGASIDWSDRNCPAPNITVKNPRNGHAHLLYALALPVRTAPDASA
                     SALRYAAAIERALCEKLGADVNYSGLICKNPCHPEWQEVEWREEPYTLDELADYLDLS
                     ASARRSVDKNYGLGRNYHLFEKVRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPV
                     PLSPAECRAIGKSIAKYTHRKFSPEGFSAVQAARGRKGGTKSKRAAVPTSARSLKPWE
                     ALGISRATYYRKLKCDPDLAK"
BASE COUNT         1554 a         1901 c         1571 g         1732 t
ORIGIN      
        1 aaaaaaaaag cgcatcagaa tcagcctgac acgctttgtt tttgtcaaag aggatttcgg
       61 gttactgcgt tgctaatccg gtcacttcag ctgttgatga cggagatacg gtcccgccct
      121 gaacatcccg gatatagttt gcctgacatg cagacagaat aatgactgca agaagcaata
      181 aaataatccc tgttattttt ttcataacga ctccttgttg tgaaagaatg ccagccattc
      241 atccataaac aaccggcatt catatttcag ccctgtttaa atcctgactt accgttagca
      301 gctcgccatt ctttaatttc cttgacaatc ccttcaggac tatcttccct gtcatcgcga
      361 ggataataaa tcagatctga accatctggg tgctcagtta atcgctcaaa ctctctcact
      421 aatttattgt catcctcttc agtagcacct tcagctctac atattttttt tacaaactcc
      481 agaaattcag cctcggtata atcactaata ctatgtttca gttccatttt tacttacccc
      541 gatgaatatc aatatgtcgc ttaggtgtgg tcactctgat attattcata tcatagacac
      601 caccatcctg actgattggt ttatcatgat gtaattcaaa gcgttcccta ccacctactt
      661 ggtctttctt ccttgcaaaa ggtgcttttc ccttttgaat gttcgtctta ttactgcctt
      721 taaattgctt actaagatcg ggatcttttg acacttcttc ccagaatttc ttccggaaat
      781 cgtcaaagtt tttaaattct ttatcacgca acttatcagc aatgcgatct ggaattggcg
      841 ctcctgaatc tttacctgca tcatccagcc atttatcacc aactggttta cctttacctg
      901 tcgccttccc tggcttattc cgtttactct ccttatctaa tttatcctta gcgtcctttt
      961 ctttattttc cttctgtttg cggcgctcct gcgcggcact taatgcagca tcagcatctg
     1021 acatctcttt tgcagcagca tcaaatgcag cctgcttatt atttacatcc gtctgcgccc
     1081 gctgggcttt aagcccggcc atttgccaca ttctgtgacc gccagccatt gggtcatggg
     1141 caaatcgatc aaattgtttt atttcagcta ttgcatcagc aagagtttta ttcgctgcat
     1201 caagttcgct tttacgcgaa ttataaacct gaacagcttt agcctgtcgc tcctgatttc
     1261 tggcaacatc ttcatttgcc tgattcagct ctgcacgcgc gcgttcataa tttcgctcag
     1321 ccgcttcaac cggatgcgta gcatcccatt cctgctgacg gcgattttct tcatcctgac
     1381 gttgttttac ctggtcaggg ctaagaacat cactcactga aacatatacg gcattatgac
     1441 cgctgtcctt cgggaatcga ataactgcat ccctggtatt accaccctga gtaaatcctg
     1501 ccgggcgaac atccttatca gtattatttg taacacctgg gcttaatgtc tgtactgctg
     1561 gcgtactgtt attaactgaa atattcagaa caggtgcacc tggaattgat gccgtaaaaa
     1621 cacctggacg ttcggtaggt tttgcatcaa ccaccggaac actcatcgga acacctgaaa
     1681 caaccgaaat attctgtcgt tcgtctttta catcatcaac aacacgaaca tttacgttta
     1741 ctgttgcctt atcgagaggt aatgaactga caggtgattc agtaatatca tctgcgggta
     1801 atgacgtcac aatctttgac atcatattgg ggtcatcttt cgctatttgt gatggcaata
     1861 caccatataa agccaccccc caaagaccaa atttaaacgg tcctttcagg gcagccataa
     1921 tatcagcaat agctgccgat aatgctcccg ctgaaatact gaccgccaga ccgccagctc
     1981 ctggagtgga aagtgccgga aaaccaaatg ccactggcgc agctactgct gacagattac
     2041 cgcctgttcc cgagccacca ccggaattac tattcccccc gccattacca tgaccggaac
     2101 caccacccca gtgaatgccg ctaccggaac caccacccca cgggttattt tccgaactcc
     2161 acccggaacc atcagaagca ccaccaccta caccaagccc ggtcgggcca ccattaatgt
     2221 taccacttgt gctatgcgcg cccgtgttat ggccgcgtcc atcgccaccg ctcataaaaa
     2281 ctccctcttt taagcgttaa aacaattaat tacatgatca atactgtaca taaaaccact
     2341 ggttttatgt acagatcaaa aagtacccgt catcgttttt tctgtcaagt tcaggtggaa
     2401 aattttttag aaatcaggag ggataacact tagaaaacgc agagcgggcc acaaaaaatt
     2461 ttgtggcccg tgatgtgttg gcggggtccg gggggcagcc ccggatatca catactcatg
     2521 ccccggaagt gcccggggcg gcgttgttgt tgcctgtgct gttcgtcccg cttccactca
     2581 tgccaggtct gattaaaacc gagttgacgg aagagataat ccggcatctc ttccggtttg
     2641 tggacagcag ccaaaagcct gtcatgatgg cctttgagcc ggaactgcat caggtgcggt
     2701 tccggtgggt acggagtatc ctcatcacgg tttatagctt ctccgtaacc tgccaggcat
     2761 accccgttct ccgtgatggt gctgactatc agatgactgt aatccggcga cggaagcaca
     2821 agaacggggc aaaaccctcc ttcccggcga acaaacccga caagcatttc ctccggccct
     2881 ttgcataaca acagattcag cgtcctgctg tttccctctc tcgaaaatac aacaggcatg
     2941 agttgttccg gtacagcatc agcggtgata tccacccggt gcgctgacgg cagcggagag
     3001 gctacgggcc tgcggtcgtc gctgaaataa aaattatccg gccagtgatg cggcagcgcc
     3061 ggaaaaccgt aagagggcca cccctggcgc atctgttcca gtacatgccg gaccgtcgca
     3121 ccggaataca ttctcacacg ctcaggccgc gccatcgtct catgagattc tggcattccc
     3181 tcgcgtatat ccgagaccat cacagccccg cctggcgaca ggtaaacatt caccgggtca
     3241 tgaacactct gggggtaatg cgccagctca tcaccccata ccgtgaattc tccggctctg
     3301 ttactcaaca tcatgtagcg ggttatcctg cccgttcccg gctctctggc acgccccagc
     3361 tccgtaataa ccccgactaa tgccgtttca cggctcagac ggtggttatc atgacgtttc
     3421 agccgcgcct catgctcctg ttccttccgg cgcgcttccg cttcggcttc ctccctgcgc
     3481 cgctccgctt cccgtgctga cagtttttct ttcagctctg ccattcccgg cgcaatccac
     3541 gtattccact gcgtttcaaa ggcctgacga cgggcgggaa tgtcccgttc tttcacggcc
     3601 ctgtaggtgc ggcgaagaga ttcaatctgt ttcccgagtt tccagaaacg gagttgtctt
     3661 ttgcggtatt ccggttcagc ccatgtcatc aggggaatct ctgacagaag ggacgcgaca
     3721 gggcgtgctt ttttgagtcc ggtcatctcc ttttcgagaa tggcctgctc tttcagcagc
     3781 gcattcccct cacgttcgat gtcgttcacc ttcccgctca tcgccaggaa ctccggggtc
     3841 atctcccacg cgttcccgca ttcctgcggc agcgtggcaa tgaattcccg cagggtatcc
     3901 gctgaggcat tcgcagcggg aatggtcagg ttaagggctt ccgctgcacg acgtgcggca
     3961 tcgctgagtt cccgtttccg cacgttctcc tgtacctgga tgactgccag ccttgtgcgg
     4021 gaaacctgta tttccaggtt aaggctgtta atcaggcggt tctgctcacc gcgttctgtt
     4081 tccatgcccc gtttttccat cgcacaggca gcctttccga ggtgaaggcc cggcgctttc
     4141 tccagtccct gacgctcgta tgaacgatgg tctatctctt cctggtagcc ggcgttcgca
     4201 agcgctctgt tcgcatggtc agcccatgat gcgcgccacg tctcggcgag tgcccgctcg
     4261 ttccagtccc tgacctttcc tgcaaatcct gtttccccga cagctctcgt ggtcagcatg
     4321 atgtgcgcat ggggattggt ccggtccatg tgatgaaacg ccacatcggc aatcatgccc
     4381 cgactgacaa agttttcccg gacaaaagcg agaacggcct cccgtgcggc ctcgcgggaa
     4441 atctcacggg gaatggcgag ttcaatctcc cttgccagct gggagttccg gcgctgttcg
     4501 gctttctcga ccgcattcca gagaacggaa cggtcactac accaggacgg tgcattcgca
     4561 ggggtgagaa tgaccgcatc atcaacgccg cgtttacggg tgtaatcgtg ggttaatccg
     4621 gtacggtcat cgggtatgac agagccggaa cggtaggcag cagaagcaac ggcactgtag
     4681 ccgttttttc gcgaaatgat tttcatgctg agatgataaa tcgccatttc tgccgtaata
     4741 cctttttgcc gttttctttt agcgtcgcag accgcacaca atcgaaacgg agtagagatt
     4801 gtataagtgc gcacttcgtg atgtgccgtg cattatgcca gttcagtttt gcaactacca
     4861 gtgacgcgct aaaatgcgat ttttcaccgc gcacggagtc acgaaatgag cagaacactc
     4921 gaacagaaga ttgccgaagc tgaggccaga ttacagcgcc tgaaggcgaa gagccggagc
     4981 ctggacacgg cgcaaaaagt cattgtgggg gctgccatgc tggcgagggt acggaggccg
     5041 gaagaagcgc aactgcgtgc gttcctgctc cagtttctgc gaaaggaagt cacaagacag
     5101 gcggatgtga accgactaca accgctgatt aacgagctgg agatgctacc cagaccaccg
     5161 gcgaaaccgc agaaccacta agccccggcg cgtaagtgcc catgcaccac cggacgcctt
     5221 tgcgcttcct cgctcattcg ctcgctacgc tcggtcatga gactgcggcg agcgctacca
     5281 cctcaatcat gcggaaagcc acgccattac ggggcagaac ggagaaaatc gcaagaaagg
     5341 tcactgctca aaaagacgcc aaaagggcgc tgttatctga taaggcttat ctggtctcat
     5401 tttgcgagat ctggatcaca ttttaatttt cggtagtacg tcgctcgact gatccctaat
     5461 gcctcccacg gcttcagcga acgtgctgat gtaggaactg ctgcgcgctt agatttagtt
     5521 ccgcccttgc gacctcgagc ggcctgtaca gcggaaaatc cctctggtga gaatttcctg
     5581 tgtgtatatt tcgcaatgct cttgccaata gcccgacatt cagccggaga aagcggaacg
     5641 ggaagcgatg cgttgtacat ttcgacacgc tggatcacgg catcaagcca ttgtgagaat
     5701 acaggccaac cctgacgaat cgctctgtag gcccatttac ggaccttttc gaacagatgg
     5761 tagtttcgcc ccagcccgta atttttatcg acgctacggc gcgctgaggc gctcaaatcg
     5821 agataatcag ccagttcgtc gagagtgtag ggttcctcgc gccattccac ttcctgccat
     5881 tcagggtggc acggattttt gcagatcagg ccgctgtaat tcacatccgc gcccagtttt
     5941 tcacacaacg cacgctcaat agcggcagcg tatctgagcg ccgaagccga tgcatccggc
     6001 gcagttctca caggaagggc gagcgcgtag agcagatgcg cgtgcccgtt acggggattt
     6061 tttacggtga tgttcggggc cggacaattc cggtcgctcc agtcgattga tgcccccatc
     6121 cggtccacgt caaagggaag ccagtacagt gcatgtggct ggttctgctg gatgaaacgg
     6181 gcaagaatgg caatgttttt cggcagaatg cgaacgccgt acgcgaaatc gttcgtacag
     6241 tacggtttgt gcggtaattt ttccctgaag cgttgaagta cggcgctcat gtgatacgat
     6301 ttctctcggt acacagttta actaaccgcg ctttcccatc gggaaacgcg gaacccggcg
     6361 ccctgtaatt tcgtgatcaa tcttggcggt gagagcacga tttacggggc gtttgttttt
     6421 cagtcgctta cactacaccc aacggcaaat cctgtcgatc cttcccgaaa acccgatcca
     6481 tttaccataa ctcccgttgt acgcccccct taaaacgccc tcaatcgctt tctgacgcaa
     6541 aaaacgcaaa aagacaagga aacaccaggg taaacataaa aaatgcgtca gaatcgtttt
     6601 tagcgcgttg cataactatg cacgaaagcg atgcgcgatc ggtttcgtgc gttctgaaca
     6661 ccgaacggct caaaacgaca agttttctcc ccctggatgg aaagttctgc acggggattt
     6721 tagatcccat ctcccacatg gaatttttgt gacatttc
//
LOCUS       DAESHI010000176         6730 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.179, whole genome shotgun sequence.
ACCESSION   DAESHI010000176 DAESHI010000000
VERSION     DAESHI010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.179"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            38..250
                     /locus_tag="KW011_004016"
     CDS             38..250
                     /locus_tag="KW011_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662228.1"
                     /translation="MSNTQKIINTEKYNEWVKKFSEQIFKITGDENVAKNELEPWTPE
                     GNAPNYCWWEVDPVDAANEAMSYHND"
     gene            283..501
                     /locus_tag="KW011_004017"
     CDS             283..501
                     /locus_tag="KW011_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HBH7662229.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            656..889
                     /locus_tag="KW011_004018"
     CDS             656..889
                     /locus_tag="KW011_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001726830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBH7662230.1"
                     /translation="MVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQRSLSERLFRE
                     QGVDTKILLGHSNQKMTDIYNDVRGKEWKKLVI"
     gene            complement(922..1560)
                     /gene="ompW"
                     /locus_tag="KW011_004019"
     CDS             complement(922..1560)
                     /gene="ompW"
                     /locus_tag="KW011_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HBH7662231.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1800..1889
                     /locus_tag="KW011_004020"
                     /pseudo
     CDS             1800..1889
                     /locus_tag="KW011_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1917..2660
                     /locus_tag="KW011_004021"
     CDS             1917..2660
                     /locus_tag="KW011_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HBH7662232.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2690..3229
                     /locus_tag="KW011_004022"
     CDS             2690..3229
                     /locus_tag="KW011_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HBH7662233.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3334..3732
                     /gene="yciA"
                     /locus_tag="KW011_004023"
     CDS             3334..3732
                     /gene="yciA"
                     /locus_tag="KW011_004023"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HBH7662234.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="KW011_004024"
     CDS             complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="KW011_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HBH7662235.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4582..6102
                     /locus_tag="KW011_004025"
     CDS             4582..6102
                     /locus_tag="KW011_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH7662236.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            complement(6135..>6730)
                     /locus_tag="KW011_004026"
     CDS             complement(6135..>6730)
                     /locus_tag="KW011_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662237.1"
                     /translation="RAPKTISISGVVVVDSTGSVLLTRQGQLVETFISTVEQWRDQKQ
                     IITIVCRDGIAITDSIITQFKASKEHTIANGLRIQMTFQGVEFRSIVGQTDVSAATGK
                     TATTNDGGATSKKNTGNTTTSLGSPMLTCKELFGYSASELSDEALKARVTCSKSVSVK
                     NGESTFSDAANEQASKVLKPGNALRKYSVNPNKKGTY"
BASE COUNT         1774 a         1538 c         1589 g         1829 t
ORIGIN      
        1 tgtaaataaa atttaatcct taactggagg tatatctatg tcaaatacac agaaaattat
       61 taacactgaa aaatataacg agtgggtgaa aaaattctct gagcagattt ttaaaattac
      121 tggcgacgag aatgtggcaa aaaatgaatt agaaccgtgg acacctgaag gaaacgcacc
      181 aaattattgc tggtgggagg ttgatccggt tgatgctgca aatgaagcca tgagttacca
      241 caacgattaa tgtcgggagg ccgcccgaaa gggcggtaag aaatgactac attattcaga
      301 aaagaatatc cgcaaaaaag tagggcgaca gaatttttgt ttctcattct gtttatcgta
      361 ttgatgatac cgatatcccc tctaattttt gtctgggcaa tcgggaaaat aattgagcca
      421 gttactgaat tgtataccga cgttgtatgg gcgtcgttca acacactgca caataaaatt
      481 aatccgtata aggaaaactg atatggcaac tttgacaaaa aaagaacggg catggttgaa
      541 cgaattacag gaagttcttg atcgctgtcc tgtggtgtca tcctgccgcg attgcgttct
      601 tagtccgtgg ctattgcacc accatcacgc gaaagggaca gctaagcgcg gcgggatggt
      661 taagccagca acattaaccg ttgcatttaa aaaagcccgg gattctgtgg attacaactg
      721 gcgtgctaat ggcaccccac cctctttcca tgagcagaga tctttatcag agcgattgtt
      781 cagagagcag ggggttgata ccaaaatttt gctaggccat tcgaatcaaa aaatgaccga
      841 tatttacaac gatgtacgcg gtaaggagtg gaaaaaactg gtcatttgat aaccagtttt
      901 gcagaggttt tgcagagaaa attaaaagcg gtacccggca gagaacataa acacccacgg
      961 atccagtcgt accgagtctt ttacggtagt aacaccagat ttgtacttag ccgtggtatc
     1021 gatatccatg taccacactg acatgttaac caaccagtca cggttaatca gataatcaac
     1081 ccccacctgc ccggcagctc cccagtaatc tttcagactg agatcggaaa gccccgcctc
     1141 tttgccatga tcgttaaatc cattatcaaa gaaggtggtg tagttaatac ctgccccaac
     1201 ataaggacgg aatttgctgc cggcatcacc aaaataccac tgcgccatca gtgttggcgg
     1261 cagatgatga acggttgcaa tatcgccggt cgcccgggtg ccgattttat ggcggaacgg
     1321 cgtcgctgcc agtaattcca caccaatgtt gtcggtcgcc atataagtaa acgtcaggcc
     1381 cagttgcgtg ttattggtca cgctgaatcc acccagactt cctaacgtac caccagcacc
     1441 ttctgttgga cgtacggttg cagaacctgc acgcataaaa aattcgcctg cttcatgcgc
     1501 aaaggcactg ccagagagaa gagttgttac tgccaaagcc gccactgtta actttttcat
     1561 atccgctccg tcgttatggt tataaaaacg tgacgaatat acctacattg gagtaacaaa
     1621 tgatcctaca tagatcacat tacatatgat aattcaccct ttattgatct ggattaattt
     1681 agaagttgca ttgagaatcc tggttaacgt gatttaaatc aatttttaga tattttcgct
     1741 acgcaaatat taactcgttc gccacttgcc gcggtttgcc cacgcctgaa ggccagcaat
     1801 tacctccgct ttacaaagat atgcttttcc atacaagacc tgatgctgcc acccgaggct
     1861 taaccagggt acaattgccc gctaattaac acctgcataa aactcaagga gagtgcatgt
     1921 ctatcacggc gcagtccgta taccgtgaca ccggaaattt cttccgtaat caatttatga
     1981 ccattctgtt ggtatcgttg ctatgtgcgt ttatcacagt ggtgttaggg catgttttct
     2041 cacccagtga tgcacagctt gcgcagctca atgacggcgt gcccgttagc ggcagtagtg
     2101 ggttgttcga cctggttcag aatatgtcac cggaacaaca acaaattttg ctgcaggctt
     2161 cagcggcgtc cactttttca ggattaatcg gtaacgccat tctcgccgga ggcgtaatat
     2221 taattatcca gctggtgtct gcgggtcaga gagtcagtgc gctgcgagct attggtgcca
     2281 gtgcgccgat attgcccaag ttatttattc tgatttttct gactaccctt ttagtacaga
     2341 ttggcatcat gctggtggtc gttccgggaa ttatcatggc cattttactg gccctggcac
     2401 cggtgatgct ggtacaggac aaaatgggca tttttgcctc gatgcgtagc agtatgcggc
     2461 tgacttgggc gaatatgcgt ctggtggcac ccgcagtact gagctggttg ctggcaaaaa
     2521 cactgttgct gctttttgcc tcttcttttg ccgcattaac cccggaaatt ggtgccgtac
     2581 tggcgaacac cttgagcaac ctgatttcag ccatattgct catctatctg ttccgcctgt
     2641 atatgttgat tcgccaataa ccttaagcat ttgattacgg aatcgtaaaa tgaagcagtt
     2701 tcttgatttt ttaccgctgg ttgtcttttt cgcgttttac aagatttatg acatctatgc
     2761 ggctactgcg gcgctgatcg tcgccacggc gattgtgctt atctatagct gggttcgctt
     2821 tcgtaaggtt gagaagatgg ccctgatcac ttttgttctg gtggtcgtct tcggtggctt
     2881 gacgctgttc ttccacaatg atgagtttat taaatggaag gttacagtca tttatgctct
     2941 gtttgcggga gccctgttag tcagccaatg ggtgatgaaa aagccgctaa ttcagcggat
     3001 gctgggtaaa gaactcacgc tgccgcaacc ggtatggtcg aagctaaatc tggcctgggc
     3061 tgttttcttt atcctttgcg gtctggcaaa catctacatc gcattctggc tgccgcaaaa
     3121 tatttgggtc aactttaaag tctttggcct gaccgccctt accttaatct tcacattgtt
     3181 aagcggtatc tatatctacc gccacatgcc gcaggaagat aaatcctaac cagactgcca
     3241 gtccttcggg gctggcgctg tatctcctct gttctcctca ggaaaatcat agtagcatcg
     3301 cgcctgtgat tttcctttta agtcggtttt accatgtcta caacacataa cgtccctcag
     3361 ggcgatcttg ttttacgtac tttagccatg cccgccgata ccaatgccaa tggtgacatc
     3421 tttggtggtt ggttaatgtc acaaatggat attggcggcg ctattctggc aaaagaaatt
     3481 gcccacggtc gcgtagtgac tgtgcgggtt gaaggaatga ctttcttacg gccggttgcg
     3541 gtcggcgatg tggtgtgctg ctatgcacgc tgtgtccaga aagggacgac atcggtcagc
     3601 attaatattg aagtgtgggt gaaaaaagta gcgtctgaac caatcgggca acgctataaa
     3661 gcgacagaag cattatttaa gtatgtcgcg gttgatcctg aaggaaaacc tcgcgcctta
     3721 cctgttgagt aatagtcaaa agcctccggt cggaggcttt tgactttcta cttactgaat
     3781 ttcggtggtg ccgttaattt taaacaggat attcaccaca atcccactgc ctggcttacc
     3841 cggctcataa cgccatctgc gcatcgcatt tttcacctca cgctcaaaca tgttcgcagg
     3901 cttggctgag aggatttgta cgttatccac gcgaccatcc ggcgtgacgt caaatttaac
     3961 tttaacctgc ccttcaatgc gcaatgcctg tgctcgtgcc ggatactgcg gctgattacg
     4021 gcttaatgcg cgtggtcctg aagccacact ggtaaccggc ttgctggttg cagccgttgc
     4081 cgtacttgat gtggggcgcg cgggtgccgt attttcaaac ggtgatgccg gacgcgactc
     4141 tacgggtttg acatcgcgtt ttggctgctc ctgtaccttt ttcaccggct tcggttttgg
     4201 cttaggtttc ggcttcggct tttcaatgac caccggtgct tcttttggcg gttcggggat
     4261 cggctcaggt tccggttctg gctctaccac cggctccggt ggcggctgaa cggcttgtgg
     4321 cggttcgaga tcagcaggcg caaccatcgt gacagaaatt ggctgcgcag gcgcaggtag
     4381 ttcaataacc tgatgtaccg aggtatagag cagacccgcc acaacagcac catgaatgca
     4441 gaccgaaagt aacgtcggcc aggggaagcg gcgaggtaaa tcaagggtca ttgtattgta
     4501 gctattcggc attgtagcat tagccataac gtttataagt tcatctttaa atgataatca
     4561 attgcatggg agtttatcac cgtgagaacc gtaataacat acttgagatt ctcatcagcc
     4621 atacaaggcg ctgaaggtgc agattcaacc agacgacaaa acgacctgtt caagcagtgg
     4681 ctgaagaaga atggcgatgc tcaaatagta gcgtctttca gtgatgaagg gttgagtggt
     4741 tacaaaggta aacaccttac tggtcagttt ggtgacatgt tagcccgtat tgaggctggg
     4801 gagtttccag aaggtacaat tctgctagtt gagagtatcg accgtatagg ccgattagag
     4861 catcttgaaa cagaagcctt gatgaaccgc atcttaggca acgggataga gattcacaca
     4921 ttgcaagatg gccttatcta cacaaaggat gctttagccg atgaccttgg cataagcatc
     4981 atccagcgtg tcaaagccta catagcgcat cagaaatcta agcagaagtc tttccgtgtt
     5041 tctcagaaat gggggcaacg tgcaaaactt gcccttgctg gtgaacaacg tcttacaaag
     5101 atggttcctg gatggattga ccctgagact tttaaactca atgaacacgc tgagactgtg
     5161 agattgatat tcaaactgct gctagatggt gaaagtctgc ataacattgc acgtcacctt
     5221 caaagcaacg gtataaagtc gtttagtcgc cgtaaagatg ctaatgggtt ctctgttcac
     5281 tctgtacgca ccattctaag gtcagagaca acaataggca cgttaccagc atcacaacgt
     5341 aatgaccgcc ccgctatacc gaactactac gaaggtgttg tagatatacc aacgttcaat
     5401 aaagctcaag agattctcga caagaatcgt aaaggccgta cacctgcaag tgacaaccca
     5461 ctaacgatta acatcttcaa aggtctgttt aggtgtcagt gtggggctag tgtccatcct
     5521 accggaacaa agaataagta tgctggggtc tacaggtgca ataaccactt agacggtcgc
     5581 tgtgatgttc caccgttgaa gcgtaaaccg tttgaccgat ggatgattga taattttctg
     5641 gggatgattg acgtggggaa tgatggagaa tcagagagaa agattgcagc gttacagcat
     5701 gaggttgaaa ttgtcacagc cagaatcaag aaagctaccg ccctacttct tgagatggat
     5761 gatattgatg aactaaaaat tcagcttaag gaactgaacc agaagcgcac agaactacag
     5821 accacgattg ataacatgag gcgtaaggct tcactcactg ataaggaact accccagctt
     5881 aaagatattg accttatgac taaagcgggt cgtgtcgagt gtcagttgat tttgtccaag
     5941 catctaaaag ggcttacatt gggtaaggat tcggtaactg taacgctaca gaacgacact
     6001 gaaatcacca tacctacaaa cccgttaccg ctaaatgatg ggtcaccaat ctttgaaatt
     6061 gctgataaag agctactgga tattgacgct tatcaactgt aattttcagg ggctatgaag
     6121 ccccttttta tgtctcaata agtgcccttc ttgttagggt tcacagagta ctttcgcaga
     6181 gcattacccg gcttaagcac ctttgaagct tgctcattag cggcatctga gaaggtgctc
     6241 tcaccatttt taacgctaac gctcttagag cacgttacac gggcttttag cgcttcatct
     6301 gataactcac ttgccgaata gccaaaaagc tctttacagg tcagcatagg gcttccaagg
     6361 ctcgttgtag tgtttccggt gttcttttta ctggtagcac cgccatcatt cgtggtagcg
     6421 gtctttccag tagcagccga aacatcagtc tgacctacga ttgaacgaaa ctctacacct
     6481 tggaaggtca tctgaatacg aagaccgtta gcaatggtat gctctttact cgctttaaac
     6541 tgagtaatga tactatcagt gatagctatt ccatctctac aaacaatagt gatgatttgc
     6601 ttctggtctc gccattgttc aaccgtagaa ataaaggttt ctacaagttg cccctgtctg
     6661 gttaatagaa ctgaacccgt cgaatcaacc actacaacac cgctaataga gatggtctta
     6721 ggtgctctct
//
LOCUS       DAESHI010000177         6696 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.180, whole genome shotgun sequence.
ACCESSION   DAESHI010000177 DAESHI010000000
VERSION     DAESHI010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6696
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.180"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..737)
                     /locus_tag="KW011_004027"
     CDS             complement(<1..737)
                     /locus_tag="KW011_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HBH7662238.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQID"
     gene            complement(878..2911)
                     /gene="metG"
                     /locus_tag="KW011_004028"
     CDS             complement(878..2911)
                     /gene="metG"
                     /locus_tag="KW011_004028"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBH7662239.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3043..4152
                     /gene="apbC"
                     /locus_tag="KW011_004029"
     CDS             3043..4152
                     /gene="apbC"
                     /locus_tag="KW011_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HBH7662240.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4397..4678
                     /locus_tag="KW011_004030"
     CDS             4397..4678
                     /locus_tag="KW011_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HBH7662241.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4970..5512
                     /locus_tag="KW011_004031"
     CDS             4970..5512
                     /locus_tag="KW011_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBH7662242.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            5592..6266
                     /locus_tag="KW011_004032"
     CDS             5592..6266
                     /locus_tag="KW011_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBH7662243.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            6282..>6696
                     /locus_tag="KW011_004033"
     CDS             6282..>6696
                     /locus_tag="KW011_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBH7662244.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELE"
BASE COUNT         1771 a         1513 c         1601 g         1811 t
ORIGIN      
        1 atatcaatct gttgaaggtg tattattaca tcagtagcat attccagacc gccttcctgg
       61 acgatctgat ccataacatc ctcacgcact ttatatcttg tgcatttcat ttcaagtgcc
      121 gtgagtacag ccattactgg tgatggtaac tgtgttgttt tcaggccgtc gatctgactg
      181 atcgcctggg cgacggtgtt ttgccattct aaagaacagg cgctgaagtc aaatgtggct
      241 gttttattat cccgttgcga cacactacag gctaagcgac gtaattgggt caatttgtct
      301 gcttgctgaa caggaactcc gggaaagcgg cgatgactta acgtggtgtg tacaacttcg
      361 ggcggaaaaa cgacagcaat atctttcgcc agccacggta aaacagcatc tgtatttttg
      421 tcaggcgcac gggtttctgc tacgaaagag ggttgttcct gaataatggt ggataagtcc
      481 gctaccttga gagagcccgt taccgtttgg gaaggttgca aagaattatc cttcgcttgc
      541 tccacatacc ccttcttcac cttctccgca atcagcttaa gctccgcttt tgccgctgcc
      601 gcagcatctg aaaaactttt tatctggctt tgccctttgg tgccaacttt tccccaactg
      661 atatgcaact cgtttccctg ttgctcaact gcccagaatt tatgtgattt ttcgtcctga
      721 tagataaagt gtctcattct gcatccctgt aattttatcc agttgaatgc agatgctacc
      781 agtatttatg cgggttagag agagacaaaa tcgcaagaaa acaactttag gaatttataa
      841 agaaaaggcg ctgtcgatgc agcgccttga agggggatta tttcacctga tggcctggtt
      901 tagcaccagc atccgggctt aacaggaaga tatctttccc gccaggacca gcagccatta
      961 ccatgccttc ggagatacca aagcgcattt tacgcggtgc caggttggcc accataatgg
     1021 tgtgacgccc gatcagtgcc tgcggatccg ggtaggcaga acgaataccg gagaagacat
     1081 tgcgtttttc accgccgaga tccagtgtca ggcgcagcag tttgtcagaa ccttcaacaa
     1141 actctgcgtt ttcgatcagc gccacgcgca ggtcaacttt tgcgaagtcg tcaaaggtga
     1201 tggtttcctg aatcggatca tctgccagtg ggccagtaac tggcgcggca gctgctttca
     1261 cttcttcttt agacgcttcc accagtgctt caacctgctt catatcgatg cggttataca
     1321 gcgctttgaa cggattcact ttgtggccca gcagcggttg ctggatacca tcccaggtca
     1381 gttctgtatt gaggaatgct tctgcgcgct cggtcagttt cggcagtacc ggcttcaggt
     1441 aagtcatcag cacgcggaac aggtttatgc ccattgagca aattgcctgc aggtcggcat
     1501 cgcggccttc ctgttttgcc accacccacg gagcctgctc gtcgacatag cggttagcca
     1561 gatcagccag cgccatgatt tcgcgcactg ctttaccaaa ttcgcggctt tcccacgctt
     1621 caccaatcac ttcagcggca tcagtgaagg ttttgtacaa ctgcgggtca gccagttcgc
     1681 ttgccagcac gccgtcaaaa cgcttgttga taaagcccgc attacgagag gccaggttaa
     1741 ccactttgtt cacgatatcg gcattcacac gctgaacgaa atcctccagg ttgagatcga
     1801 tatcatcaat gcgcgaagag agtttcgcag tgtagtagta acgcaggctg tcagcgtcaa
     1861 aatgattcag ccaggtgctg gctttaataa aggtgccgcg agacttggac atctttgcgc
     1921 cgttcaccgt cacatagcca tgaacaaaca ggttggtcgg cttgcggaag ttgctgcctt
     1981 ccagcatggc aggccagaac aggctgtgga agtaaacaat atctttaccg atgaagtggt
     2041 acagctcggc ggtggagtct ttcttccagt attcatcgaa gcttacgctg tcgctgcgct
     2101 tgtcgcacag attcttgaaa gaacccatgt agccaatcgg tgcgtccagc cagacgtaga
     2161 aatatttgcc cggcgcgttc ggaatttcaa aaccaaagta tggcgcgtcg cgggagatat
     2221 cccactgttg caggccggat tcaaaccact cctgcatttt atttgccacc tgctcctgca
     2281 acgcaccgct gcgggtccat gcctgcaaca tttcgctgaa agagggcaga tcaaagaaga
     2341 agtgttcaga atcacgcatt accggcgtag cgccagaaac caccgatttc ggctcgatca
     2401 attcagtcgg gctgtaggtc gcgccgcaga cttcgcagtt atcgccgtat tgatccgggg
     2461 atttacattt cgggcaggtg cctttcacaa aacggtccgg caggaacatg cctttttccg
     2521 gatcgtacag ctgagagatg gtgcggtttt taataaaacc gttctctttc aggcgagagt
     2581 agataagttc tgacaactgg cggttctctt cgctgtgcgt cgagtgatag ttgtcatagc
     2641 tgatgttaaa gcctgcgaaa tcagtctgat gctcctgact catttcgcca atcatctgct
     2701 ccggggtgat accaagctgc tgagctttca gcatgatcgg tgtaccgtgg gcatcgtcgg
     2761 cgcagatgaa gttgacctcg tggccgcgca ttcgctggta acggacccag acatcagcct
     2821 ggatgtgctc cagcatatgg ccgaggtgga ttgagccgtt agcgtacggc agtgcgcacg
     2881 tcaccagaat tttcttcgcg acttgagtca tagtaggcat tacttcttaa tagtgaaaag
     2941 gggttagata gtaccaaatg ggaaaatgtt aagtaagtac ttctctatat tcttcaggta
     3001 aactatcagc tgatggttta tctatacaaa aaaggagtcg ggatgaacga acaatcccag
     3061 gccaaatcac cagaagcctt gcgcgcaatg gtcgccggga cactggccaa ttttcagcac
     3121 ccaaccctga agcataacct caccacgctg aacgcgttgc accatgtcgc ctggatggac
     3181 gacacgttgc atgtggaact ggttatgcca ttcgtctggc atagcgcgtt tgaagagtta
     3241 aaagagcaat gtagcgccga actgctgcgt atcaccgggg cgaaagctat cgactggaaa
     3301 ctgtcgcaca atatcgccac cttaaaacgc gtgaagaatc agccgggtat taatggtgtg
     3361 aagaacatca ttgcggtcag ttccggtaaa ggcggcgtgg gtaaatcctc cacggcggta
     3421 aacctggcgc tggcactggc ggcggaaggg gcgaaagtcg gcattctgga cgccgatatc
     3481 tacggcccat caattcccac catgctgggc gcagaaaatc agcgtccaac ttcgccggac
     3541 ggtacccata tggcaccgat tatgtctcat ggcctggcaa ccaattcaat tggttatctg
     3601 gtcactgacg acaatgcgat ggtgtggcgt ggaccgatgg cgagcaaggc gttaatgcag
     3661 atgttgcagg aaaccttgtg gccggatctc gactatctgg tgcttgatat gccgccaggc
     3721 accggtgaca ttcaattgac gctggcgcaa aacattccag taactggtgc agtcgtggtc
     3781 accaccccgc aggacatcgc tttgatcgat gcgaaaaaag gcattgtgat gttcgagaaa
     3841 gtcgaggttc cggtgcttgg tattgtcgag aacatgagtg tgcatatttg cagtaactgt
     3901 ggtcaccatg aaccgatttt cggtaccggt ggtgcagaga aactggctga aaaataccac
     3961 acacagctgc tggggcagat gccgttacac atctccttac gtgaggatct cgataaaggc
     4021 acgccaacgg ttatcagccg tccggagagc gaatttaccg ctatctaccg ccagctggct
     4081 gaccgcgttg cagctcagct ctactggcag ggtgaagtca ttccaggcga gatttccttc
     4141 cgcgcggtct aacgcctatt aaccattctg gttattcttc atacaccgca gagatgttac
     4201 attgatgcgg tgtattagtt tttgccgcat taatgaacac ttatttaata ctgcgaatat
     4261 tctgcggacc ttcgtccgaa actctacaaa gtcgattgat catacaggca atgcttcatt
     4321 atcatccttc gtggctgtgt agtggatgta cagctatagt ttgttatgca ctcattctat
     4381 ccaggcataa ccttcaatga ataagtattg gctgtcagga attatttttc ttgcctatgg
     4441 attggcttct cccgcattct cttcagagac tgccacatta gcgattaatg gcaggatcag
     4501 tcccccaaca tgcagtatgg ctatggttaa tggtcaacct caaccgcatt gtggtcagtt
     4561 aacgtataat gttgacaccc gtcatctgtt ttcttcgccc gtcaaaggtg taacgacaga
     4621 agtggttgtt gcaggtagcg atagcaaacg tcgaatcgtg ttaaatcgct acgattaatt
     4681 tggcaacctg attttaattg catttagaaa gccgaaatca tttatataaa tgatttcggc
     4741 ttttttattg atatcaacaa taccatttac ataaatctac attaattgat agatgcgata
     4801 aatagttaat actcttaaat atttgttgta tttatattgc aatggttaat ttgtttcaaa
     4861 taaataaaac aatgtgttta cattgcggta acttttttga tgtgagaaat ttgtttccaa
     4921 gagatacaga ctcttaacaa ccaaaaaaaa ttttattaag gaacatatta tgaaacgttc
     4981 aattattgct gccgctgtct tttcttcttt ttttatgagc gctggagtat ttgctgcaga
     5041 cgttgatacc ggaacattaa ctattaaggg gaatattgca gaatctccgt gtaaattcga
     5101 agcgggtggt gattcagtaa gtattaatat gccgactgta ccaaccactg tctttgaagg
     5161 taaagctaaa tattctacct atgatgatgc agtcggtgta accagcagca tgttaaaaat
     5221 tagctgcccg aaagaagttg ctggtgtaaa actctcgttg attaccaacg ataaaataac
     5281 cggtaacgat aaggcgatag ccagtagcaa cgataccgtg ggttactatc tctatttagg
     5341 tgataacagc gatgtcctgg atgtttctgc accttttaac attgagagtt ataaaacagc
     5401 ggaaggtcaa tatgctattc cgtttaaagc aaaatacctg aaactgacag ataactcagt
     5461 gcaatcaggt gatgtgttat cttctctggt tatgcgtgtg gcgcaggatt aataaataaa
     5521 attgtaagtt tctccaggta ataaacatgg ccgctatccc atggcggcct tttaatttaa
     5581 gaggcattaa aatgaaagga ttattatctt tactcatttt ttctatggtc cttcctgcac
     5641 atgccggaat tgttatctac gggacgcgca ttatttaccc ggcagaaaat aaagaagtga
     5701 tggtgcagtt gatgaaccag ggaaaccgtt cttcgctgct gcaggcgtgg attgatgatg
     5761 gcgatacgtc attaccacca gaaaaaattc aggttccttt catgttaacg ccaccagtgg
     5821 caaaaatagg ggcaaattcc gggcagcaag taaaaatcaa aattatgccg aataaactgc
     5881 ccactaataa agaaagcatt ttttatctga atgttctgga tattccacca aatagtccag
     5941 agcaagaagg taagaatgca ctgaagtttg cgatgcaaaa cagaattaag ttgttttacc
     6001 gaccagcggg tattgctccg gtaaataaag cgacatttaa aaaattgctg gtaaatcgca
     6061 gtggcaatgg tttggtgata aaaaatgact cagctaattg ggtgacgatt tcggatgtca
     6121 aagctaataa tgtcaaagtc aattatgaaa ctattatgat tgccccctta gaaagtcaga
     6181 gtgttaatgt caaaagtaat aatgcaaata actggcatct gaccattatc gatgaccatg
     6241 gcaactatat tagtgacaaa atttaattgc aggagctgcc tatgttgaga atgaccccac
     6301 ttgcatcagc aatcgtagcg ttattgctcg gcattgaagc ttatgcagct gaagaaacct
     6361 ttgataccca ttttatgata ggtggaatga aagaccagca ggttgcaaat attcgtcttg
     6421 atgataatca acccttaccg gggcagtatg acatcgatat ttatgtcaat aagcaatggc
     6481 gcgggaaata tgagattatt gttaaagaca acccgcaaga aacatgttta tcaagagaag
     6541 ttatcaagcg gttaggcatt aatagcgata acttcgccag cggtaagcaa tgtttaacat
     6601 ttgagcaact tgttcagggt gggagctata cctgggatat cggggttttt cgtctcgatt
     6661 tcagtgtccc gcaggcctgg gtggaagaac tggaaa
//
LOCUS       DAESHI010000178         6553 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.181, whole genome shotgun sequence.
ACCESSION   DAESHI010000178 DAESHI010000000
VERSION     DAESHI010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.181"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            142..1317
                     /gene="mdtL"
                     /locus_tag="KW011_004034"
     CDS             142..1317
                     /gene="mdtL"
                     /locus_tag="KW011_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HBH7662245.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1292..2251
                     /gene="yidZ"
                     /locus_tag="KW011_004035"
     CDS             1292..2251
                     /gene="yidZ"
                     /locus_tag="KW011_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HBH7662246.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2408..3157
                     /locus_tag="KW011_004036"
     CDS             2408..3157
                     /locus_tag="KW011_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HBH7662247.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MGLQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3179..3745
                     /gene="yieF"
                     /locus_tag="KW011_004037"
                     /gene_synonym="chrR"
     CDS             3179..3745
                     /gene="yieF"
                     /locus_tag="KW011_004037"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HBH7662248.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDRQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(3799..5136)
                     /gene="adeP"
                     /locus_tag="KW011_004038"
     CDS             complement(3799..5136)
                     /gene="adeP"
                     /locus_tag="KW011_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HBH7662249.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5302..5967
                     /gene="yieH"
                     /locus_tag="KW011_004039"
     CDS             5302..5967
                     /gene="yieH"
                     /locus_tag="KW011_004039"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HBH7662250.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            6034..6507
                     /locus_tag="KW011_004040"
     CDS             6034..6507
                     /locus_tag="KW011_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HBH7662251.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1608 a         1655 c         1659 g         1631 t
ORIGIN      
        1 atttggctaa atccttcaag aagccagcca ttcgctggct tcttgcctct caggaaatca
       61 cttatgtcca aatggcaact cgcctgatcc tccttcacca cgtatgcttt gcgtcacctt
      121 actatcagga cgctttagcc catgtcccgc tttttgattt gtagttttgc cctggtttta
      181 ctttatcccg ccgggattga tatgtacctc gttggtttac cgcgcatcgc cgccgatctc
      241 aatgccagcg aagcgcagtt gcatattgcg ttctccgtat atctggcggg gatggcagct
      301 gcgatgttat ttgccggtaa agtggctgat cgttcaggga gaaagccggt cgccataccc
      361 ggcgcggcgc tatttattat tgcctcggtg ttctgttcac tggctgaaac cagcacgtta
      421 tttcttgcag gccgatttct acaggggttg ggcgcaggct gttgttacgt agtggcgttc
      481 gccattttgc gcgacacgct ggatgatcga cgtcgggcta aagtgctgtc attactcaac
      541 ggtattacct gcatcattcc ggtgttagcg ccagtgctcg gacatctgat tatgcttaaa
      601 ttcccgtggc agagtctgtt ctgggcgatg gcaatgatgg gcatcgcggt actgatgttg
      661 tctttgttta ttttaaaaga aacgcgccca gcgtcccccg ctgcttcgga caaaccacga
      721 gaaaatagcg agtcgctgct taatcggttt ttcctcagcc gtgttgttat caccaccctc
      781 agcgtttcgg tgatcctcac tttcgtcaat acatcgccgg tattgctgat ggaaatcatg
      841 ggttttgagc gcggagaata cgccaccatt atggcgttga ccgctggcgt cagcatgacc
      901 gtttcattct ccacgccatt tgcgctggga atttttaagc cacgtacgtt gatgatcacc
      961 tcgcaggtgt tattccttgc ggcggggatc acccttgccg tttcaccttc ccatgcggtt
     1021 tctctgtttg gtatcacgct gatttgcgcc ggtttctcgg taggttttgg cgtagcgatg
     1081 agtcaggcgt taggaccatt ttcattacgc gcgggcgtag ccagctcgac cttaggtatt
     1141 gcgcaggttt gcggttcgtc actgtggatt tggctggcag cggtggttgg tattggcgca
     1201 tggaatatgc tgatcgggat tctgattgcc tgtagtatag tgagcctgtt gctgattatg
     1261 ttcgtcgcgc ctggacgccc cgttgccgct catgaagaaa tccatcacca cgcttgatct
     1321 caatctgctg ctctgtctgc aactgctgat gcaggagcgc agcgtgacca aagcggcgaa
     1381 gcggatgaac gtgacccctt cagcggtgag taagtcgctg gcaaagttaa gagcgtggtt
     1441 tgacgacccg ctctttgtga actcaccgct gggcctgtcg cccacaccgc taatggtcag
     1501 catggagcaa aatctggcgg aatggatgca aatgagcaat ctgctgctgg ataaaccgca
     1561 ccaccagaca ccgcgcggcc tgaagtttga gttggcggcg gaatcgccgc tgatgatgat
     1621 catgcttaat gcgctgtcga aacggatcta ccaacgttac ccgcaggcga ccatcaaact
     1681 acgtaactgg gattacgatt ccttagatgc cattactcgt ggtgaagtgg atattggttt
     1741 ttccggtcgc gaaagccatc ctcgctcgcg ggagctgtta agctcgctac cgttagccat
     1801 tgattatgaa gtgctgttta gtgatgtacc ctgcgtctgg ttacgcaaag atcatccggc
     1861 actgcatgaa acgtggaatc tggacacctt tttacgttat ccgcatatca gcatttgctg
     1921 ggaacagagc gacacctggg cgctggacaa tgtgttacag gagctggggc gcgaacgcac
     1981 gattgctatg agtctgccgg aattcgagca gtcactgttt atggcagcgc aacccgacaa
     2041 tctgctactg gcgaccgcgc cgcgctactg tcagtactac aatcaactcc atcaactgcc
     2101 gttggttgct cttcctctcc cgtttgacga aagccagcaa aaaaagctgg aagttccttt
     2161 taccctgctg tggcataaac ggaacagcca taatccgaag atcgtctggt tacgggaaac
     2221 cattaaaaac ctttacgcgt cgatggcata accgaatcgt atgaaacgcg acccaatttc
     2281 acaataaaat gtaaaaaagt tgtaacaagc ttgtctgaat cgaactttta gccgctttag
     2341 tctgtccatc attccagtaa atgattactc ttgtattcat aatggaccat taagcatgga
     2401 gcgaaaaatg gcgactcact ttgcccgagg gattttaacg gaaggacatc tgatttctgt
     2461 tcgtctcccc tcccagtgtc atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg
     2521 ttttctggcg tccagaggtt tactcgcaga actgatgttc atgctgtatg gcattggcga
     2581 attgccggaa atcgtcaccc tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc
     2641 ttcgttttct atttcctatg ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga
     2701 atgtggcctc gatatgggac tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc
     2761 cgataaccac accttttcca gcaatgaatc gctatggatc agtaaacaaa acgatcctaa
     2821 cgaagcgcgg gcgcagctca tcacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt
     2881 tttgaatgac gatccgcgcg atctgcagct gctgcccatt gccggacgcc tgaaatgtgc
     2941 tcatgtaaat catgtagaag cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc
     3001 ggtcacgccc gcgattgaaa agctcagtgt ctgggagtta gatggcaaac acagctggaa
     3061 aagcctgccg gatattcaca gccgcgccaa caatcctacc agccggatga tgcgttttgc
     3121 ccagctctct accgtgaagt ctttttcgcc aaattgatag acaaccacag gagtcattat
     3181 gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg cgcaaaggct catttaatgg
     3241 catggttgca cgtaccctgc cgaaaattgc tccggcgagc atggaagtca atgcgttacc
     3301 ttccattgcc gacattcccc tgtatgacgc tgacgtacag caggaagaag gttttccagc
     3361 aacggttgaa gctctggcgg aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc
     3421 ggaatataac tactcggtac cgggtgggct gaaaaatgcc atcgactggc tttcccgcct
     3481 gccggatcaa ccgctggccg gtaaaccggt gttgattcag accagctcaa tgggcgtgat
     3541 tggcggcgcg cgctgtcagt atcacctgcg ccagattctg gttttcctcg atgcaatggt
     3601 gatgaacaag ccggaattta tgggcggcgt gattcagaac aaagttgatc ggcaaaccgg
     3661 agaagtgatt gatcagggta cgctggacca cctgaccggg caattgaccg catttggtga
     3721 gtttattcag cgagttaaga tctaaataaa aaacccgcca gtaatcatgc atggcgggtt
     3781 tttaatgcgc tatcgatttt aatgagcgtc gataaataca atcttcagga taaacagcag
     3841 cgcaacgatg attacgcacg ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac
     3901 gcagtaggag ataaagccca gcgcaatacc ttcggtaatc gaaaagctga acggcatcat
     3961 cacggcggta ataaacgccg gaacagattc agtaagatcc tgccagttca cgcgagcaag
     4021 acttgaggtc atcaacacgc caacgtagat caacgcgcca gctgcagcgt agcctggcac
     4081 cattcccgcc agcggcgaca gaaagataac cagcaggaac agcagaccaa caaccactgc
     4141 cgtcagaccg gtacgaccgc caaccgatac gccggaagag gactcaatat aagccgtaac
     4201 ggaagaagta ccgataaacg aaccggtcac ggaagagata ctgtcgacat acagcgcctg
     4261 cttcatgcgc gggaatttcc ccttctcatc cgccagacct gctttatcgg tcacgccaat
     4321 cagcgtaccg gaggagtcaa acaagttgac caacatgaaa gagaaaatca cccctgccag
     4381 cccgaggtta aacgacccgg ctaaatctac atgacccaca actgtcatta cgctcggcgg
     4441 cgcagaaacg atgccattgt agtgcacatc acccagcatc cagcccagca gcgtcgtcac
     4501 cacgatagaa accagcaccg ctgcgtgaat gttgcgcgag gccagaatgg cgatgatgaa
     4561 gaaaccgagg atccccagca gcacgctgtg agaagtcaga ttaccgatgc tcaccagcgt
     4621 ttccgggtta gcgacaatca cacctgcgtt tttcagcccc atcatgccaa tgaacagacc
     4681 gataccgctg gtaataccca cacgcagact taccggaatg ttagcaatca tccagtaacg
     4741 aacgcggaaa atggtcaaaa gaagcagtcc gacagcgccc cagaagattg cgcccatacc
     4801 gatctgccac ggcagcccca ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc
     4861 catcgcagga gccagcgcca ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa
     4921 agcagcaatc agacaggttg ttacgaagac ggcactggtg tccatgccag caacgcccag
     4981 aatttgtggg ttaacaaata cgatgtagac catcgtcagg aaggtggtga acccggcaat
     5041 cacttcagtt cgtgccgtag taccatgttc gcgcagttta aaaactcgtt ccagcatccc
     5101 ctgaccagaa gtctgggtgg tatgttgatg actcattatc tatttccgaa caaggaggga
     5161 aaatccgtcg ctatcgtata ccaaaatgcg acaataggcg cgtttgtgag agactttttt
     5221 tattggattt acttatacgg caacgattgc gttgcgcaaa tcggctctac gaaaacgtta
     5281 aactgattaa aaaggaaagg catgtcccgg atagaagcgg tatttttcga ctgcgacggt
     5341 acgctggtcg acagtgaagt catttgctcc cgcgcatatg taacgatgtt tcaggaattt
     5401 ggtattacgc tcgatcctga agaggtattc aaacgtttca aaggtgtaaa actgtacgaa
     5461 attatcgata ttgtttccct tgaacatggt gttacgttag cgaaaacaga atctgaacac
     5521 gtttaccgtg cagaagtcgc tcggctgttc gattcagaac tggaagccat cgaaggggct
     5581 gaagcgctcc tgtcagcgat cactgcgcca atgtgtgtgg tatctaacgg cccaaataac
     5641 aaaatgcagc attctatggg caagctgaat atgttgcact acttcccgga taaactgttc
     5701 agcggctacg atattcagcg ctggaagcca gacccggcgt taatgttcca tgcggcaaaa
     5761 gcgatgaatg taaatgtaga aaactgcatt ctggttgatg actcagttgc cggtgcgcaa
     5821 tctggtatcg acgcaggtat ggaagtgttc tacttctgcg ccgacccgca caataagccg
     5881 atcgttcacc cgaaagtcac cacctttacc catctttcgc agttaccgga actgtggaaa
     5941 gcgcgtggtt gggatattac ggcatagttc ttcacactcc cttcacttac cccgcttaaa
     6001 ttggcgctca aaggtaagta aagggagttt gatatgtctg tttcacgtcg ggtaatacat
     6061 cacggacttt attttgcggt tttaggaccg ttaattggtg ttctgtttct tgtcctctac
     6121 atattcttcg caaaagaacc gctggttctt ttggtaataa tacaggtact tccattgttt
     6181 ctcttattgt cgattactac gggagctatt cctgcattgt taaccggcgt gatggtcgcc
     6241 tgtctgccgg aaaagatcgg gtcacagaaa aattatcgtt gtctggctgg tggcataggt
     6301 ggcgtcgtta tcaccgagat ctattgtgca gttattgtac atattaaggg catggcttcc
     6361 tcggagttgt ttgaaaacat tctttctggt gacagtctcg ttgtccgcat cattcctgca
     6421 ttgctggcag gtgtggtgat gagcagaatc attacccgtc tgcccggatt ggatatttca
     6481 tgtcctgaaa cagactcttt aagttaagcg ggatacttta tctttgggct actcaaaagc
     6541 agacaggatg tta
//
LOCUS       DAESHI010000179         6499 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.182, whole genome shotgun sequence.
ACCESSION   DAESHI010000179 DAESHI010000000
VERSION     DAESHI010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.182"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            171..1769
                     /gene="rtcR"
                     /locus_tag="KW011_004041"
                     /pseudo
     CDS             171..1769
                     /gene="rtcR"
                     /locus_tag="KW011_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1751..2509)
                     /locus_tag="KW011_004042"
     CDS             complement(1751..2509)
                     /locus_tag="KW011_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HBH7662252.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="KW011_004043"
     CDS             complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="KW011_004043"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HBH7662253.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="KW011_004044"
     CDS             complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="KW011_004044"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HBH7662254.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3917..5422
                     /gene="glpD"
                     /locus_tag="KW011_004045"
     CDS             3917..5422
                     /gene="glpD"
                     /locus_tag="KW011_004045"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HBH7662255.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5474..6079)
                     /locus_tag="KW011_004046"
     CDS             complement(5474..6079)
                     /locus_tag="KW011_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662256.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(6082..>6499)
                     /locus_tag="KW011_004047"
     CDS             complement(6082..>6499)
                     /locus_tag="KW011_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662257.1"
                     /translation="QRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINSV
                     PGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIENP
                     LKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
BASE COUNT         1638 a         1695 c         1663 g         1503 t
ORIGIN      
        1 ttgtttgctg tcgtgataat tgcaaccgtc gtgccagaaa attaaataag cagattaatt
       61 ttttaattca ttgtttttaa ataggttaaa tctttacgtc cgtaaacgga gattttccac
      121 aaaaccaatt caccgataat gaaatatcgt cttttataag gatatctaag atgcgtaaaa
      181 cagtggcttt tggctttgtc ggtaccgtgc tggattatgc cggacgcggc agtcagcgct
      241 ggtcgaaatg gcgtccgacg ctttgtttat gccagcaaga atcgttagtc atcgatcggc
      301 tggaattgtt gcacgacact cgctcgcgct cgctattcga aacgcttaaa cgcgatatcg
      361 ccagcgtttc gccagaaaca gaagtcgtgg gcgttgagat tgaactgcat aacccgtggg
      421 atttcgaaga ggtttacgcc tgcctgcatg atttcgcccg tggttacgag tttcagccag
      481 aaaaagaaga ctatttaatt cacatcacca ccggcaccca cgtcgcgcag atttgctggt
      541 ttctgctggc ggaagcgcgt tacctgcccg cccggctgat acaatcttca cctccgcgca
      601 aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat cgatctcgat ttaagccgtt
      661 ataacgccat cgccagccgc tttgccgagg aacgccagca aacgcttgat tttcttaagt
      721 ccggcattgc cacgcgtaac ccccacttca accgcatgat tgagcagatc gaaaaagtgg
      781 cgatcaaatc ccgcgcgccg attctgctta acggtccaac cggcgcgggc aagtcatttc
      841 tggcgcgacg catcttcgag ttaaaacagg cgcggcatca gtttagcggc gcgtttgtgg
      901 aagtgaactg cgccaccctg cgcggcgata ccgccatgtc gacgctgttt ggtcatgtaa
      961 aaggcgcgtt taccggggcg cggtaatctc gtgaaggttt attacgcagc gccaacggcg
     1021 gaatgttgtt tcttgatgag attggcgaac tgggcgcaga cgaacaggca atgctgctaa
     1081 aagccattga agagaaaacc ttttacccgt ttggcagcga tcgccaggtg agcagtgatt
     1141 ttcagcttat cgccggaacg gtgcgcgatt tgcgccagct ggttgccgaa ggcaaatttc
     1201 gcgaagatct gtacgcgcgg atcaatctct ggaccttcac cctgccgggt ctacgccagc
     1261 gccaggaaga tattgaaccg aaccttgatt atgaagtgga gcgccacgcc acactcactg
     1321 gcgacagcgt gcgttttaac accgaagcgc ggcgcgcctg gttggctttt gcgacctctc
     1381 cccaggcaac atggcgcggt aactttcgcg aactttctgc cagcgtcacg cggatggcca
     1441 cctttgccac tagcggacgc atcactctgg acgtggttga agatgagata aaccgtctgc
     1501 gctataactg gcaggagagc cgcccctccg cgcttacggc gttgctgggc gcagaggcag
     1561 aaaacatcaa tctcttcgac cgtatgcaac tggaacacgt tatcgctatc tgccgccagg
     1621 caaagtcgct ttctgcagcc ggacgacagc tttttgatgt ttcgcgccag ggcaaagcca
     1681 gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acgtgggaag
     1741 ccgtgcagga tcagcacagc tccagttgaa tatgatggtc cgtcagcacc tgcattacgc
     1801 ttactggcgg cggggcgtcg gtgtagacgg catctaccat gctgatgctg cccatattga
     1861 ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag cataacgtgg cgcgagttct
     1921 caataatggc gcgcttggtg cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc
     1981 tatcgatgcc gcttatcccc agaatgccga aatcaaggcg gaactgggag ataaaatcga
     2041 gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg gcgagaataa
     2101 tgcggaaatc ttcttttacc atcaacgtgt tagcaacatt gagattgttg gtgacaatac
     2161 gcaaattgct gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga
     2221 taaacagtgt cgagccgttg gggatttgct ccgccacttt gcgggcaatc ctctcttttt
     2281 cttcggtctg ggtggctttg cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg
     2341 ccgcaccgcc atggtgacgc aggatcaggt tttgctccgc cagctcattg aggtcgcggc
     2401 gaatagtctg cgggctgacg gagaaatgct ctaccagctc ttcagtactg acataaccct
     2461 gctgtttaac cagttcgata ataccgttgt gacgttgtgt ttgtttcatt tataaatccc
     2521 tggaattatt ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg
     2581 gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg
     2641 gcgacaatcc agatcagcgc aaagataatt aacccacgtt gcaggtaaat gccactttgc
     2701 ggatcgcgtt caccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc
     2761 ccgccaaacc acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg
     2821 agagtaataa caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac
     2881 caccaccaga gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg
     2941 aagtaacgcc agaactcaaa tttcagtgct ggatcgaatg gccaggccag ccataacatc
     3001 acttcctgat cgccgagaat ttgcatggca ataaacacca ccacgcaggc gatcatcatc
     3061 acccaggtca ccggacctgc gcgttcacgc aaggcggcaa agaaaggata acggcgataa
     3121 tgcaggccac tgccggtatg gcctgcctgc cagctggccg ccagataacg cggatctgcc
     3181 gggttttcga gaaaacgcgc cagctccgcc cgtacgcgct cggcctggga ctcatccgcc
     3241 agccagacat cgctttggtt atgttgttga atcgtgagga taacaccctg cgtcgccatg
     3301 taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat caacatcgtt
     3361 gctgtcgctt attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct
     3421 ctgcgggaaa ctgacgttgc caggcttcaa agccgccgtc aatgctatag accacatcgt
     3481 agccctgttg cagcagatac tgcgccgcgc ctttgctgct attgccgtga taacacatca
     3541 ccatcaccgg agtgtcaaag tcgttatcac gcataaacgc gcccagcgtg tcgttggtta
     3601 aatggaaagc ctgcaccgca tgccccatag cgaaactctg tggatcgcga atatcgacca
     3661 gcaccgcctc tttttcctgc aacttctggt gcgcgtcggc aacgttaata cattcgaact
     3721 gatccatgcg tctctctttc tttacaaaca agtgggcaaa tttaccgcac agtttacgtc
     3781 gaagcggcag ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt
     3841 acctaaagcg cgattctttg ctaatatgtt cgataacgaa catttatgag ctttaacgaa
     3901 agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg
     3961 tgctggtatc gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca
     4021 ggatctcgct tgcgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta
     4081 ccttgagcac tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct
     4141 gaaaatggct ccgcatatcg ccttcccgat gcgttttcgc ctgccacatc gtccgcatct
     4201 gcgcccggcg tggatgattc gcattggtct gtttatgtac gatcatctgg gtaaacgcac
     4261 cagcctgccg ggatcaactg gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat
     4321 taagcgcgga ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa
     4381 cgcccagatg gtggtgcgta aaggcggcga agtgcttact cggactcgcg ccacctctgc
     4441 tcgccgcgaa aacggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata
     4501 tagctggcaa gcgcgcggct tggttaacgc caccggcccg tgggtgaaac agttcttcga
     4561 cgacaggatg catctgcctt cgccttatgg cattcgcctg atcaaaggca gccatattgt
     4621 ggtgccgcgc gtgcataccc aaaagcaggc ctacattctg caaaacgaag ataaacgcat
     4681 tgtgttcgtg atcccgtgga tggacgagtt ttccatcatc ggcaccaccg atgtggaata
     4741 caaaggcgat ccgaaagcgg tgaagattga agagagtgaa atcaattacc tgctgaaagt
     4801 gtataacacg cactttaaaa agcagttaag ccgtgacgat atcgtctgga cctactccgg
     4861 tgtgcgtccg ctgtgtgatg atgagtccga ctcgccgcag gctattaccc gtgattacac
     4921 ccttgatatc catgatgaaa acggcaaagc accgctgctg tcggtattcg gcggtaagtt
     4981 gaccacctac cgcaaactgg cggaacatgc gctggaaaaa ctgacgccgt attatcaggg
     5041 cattggcccg gcatggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg
     5101 cgacgattac gcagctcgtc tgcgccgtcg ctatccattc ctgactgaat cgctggcgcg
     5161 tcattacgca cgcacttacg gcagcaacag cgagctgctg ctcggcaatg cgggggcgat
     5221 aagcgacctc ggggaggatt tcggtcatga gttctacgaa gcggaactga aatacctggt
     5281 ggatcacgaa tgggtccgcc gcaccgacga tgccctttgg cgtcgcacaa aacaaggcat
     5341 gtggctgaat gcggatcaac aatctcgtgt gagtcagtgg ctggtggaat atacgcagca
     5401 gaagttgtcg ctggcgtcgt aaggatgatg cccggttgaa gtgcaaccgg gctatttgga
     5461 ttccttagac tattcattgt aaataattga ataacggccc ctgagctaaa atcgccaaac
     5521 ttaagcacgt tacccaatcg ccccacttct tcctctggtt tctatttttt ctgcaccaat
     5581 ggcaataata tcccaatgca aaaccaagct gtgcactaat cgggccgaca aaatcagtcc
     5641 aaggctgcgg ccaaaaaggg gaaaaattca ggggaggcca gacaaacgtc agaaagaaat
     5701 ttcggatatt ttccagcaac gctgatttct ctggagaaat ataaagaatg aaattcgaga
     5761 gaattaacca gaaatagacc ggcgaaaaga ccagccagca atgcagtctg ttttttaatt
     5821 caggaggcgt tctgcgggag aattgccaac cgatgccagc cattacactg aaaaaaagtg
     5881 catattcaac cattaactca agttgtaatt cctgaagaga aatacgcaca accaggagcg
     5941 gaaaggagaa tattatttct gccagaaaag ttaacgtgaa tataagcacg gctaagacaa
     6001 taaccgtcgg ccataccaat aatggcacga taacaaaacc gagaattaca gggggaatat
     6061 aaagccggaa tttaatcatt tttattttcc tggtgattcg gatgatgcgt catacatcgc
     6121 gcataaacac tcatcaatta tccagtcatc ttcccagacc tgatattctg tcattggttc
     6181 gggctgtttt agcgggttct caatcgcttg ccgcgctctt tctttcagta aaggatgatt
     6241 atttctagcc caggcattga tttgggtggt aatggcgtta tcgatatcgt cctggttatc
     6301 tctgatataa tcctcacaat atctgttcaa taaacaggca agagaaacgg tcgaccaaaa
     6361 gccgggaact gaattaatgg catccagcaa tggcgcaaga gcgatatcgg cacggaaaaa
     6421 catttccagc atatactgta attcgacagc ttcatcgccg gatacccaat cagtcatata
     6481 ctggcaggca aatcgctgt
//
LOCUS       DAESHI010000180         6371 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.183, whole genome shotgun sequence.
ACCESSION   DAESHI010000180 DAESHI010000000
VERSION     DAESHI010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6371
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.183"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..314
                     /locus_tag="KW011_004048"
     CDS             <1..314
                     /locus_tag="KW011_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662258.1"
                     /translation="ALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYS
                     GAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDSQR
                     A"
     gene            416..1870
                     /gene="amn"
                     /locus_tag="KW011_004049"
     CDS             416..1870
                     /gene="amn"
                     /locus_tag="KW011_004049"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HBH7662259.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIDIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2201..2917
                     /locus_tag="KW011_004050"
     CDS             2201..2917
                     /locus_tag="KW011_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBH7662260.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3370..3445)
                     /locus_tag="KW011_004051"
     tRNA            complement(3370..3445)
                     /locus_tag="KW011_004051"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3410..3412),aa:Asn,seq:gtt)
     gene            complement(3546..5189)
                     /gene="yeeO"
                     /locus_tag="KW011_004052"
     CDS             complement(3546..5189)
                     /gene="yeeO"
                     /locus_tag="KW011_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HBH7662261.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            5194..5269
                     /locus_tag="KW011_004053"
     tRNA            5194..5269
                     /locus_tag="KW011_004053"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5227..5229,aa:Asn,seq:gtt)
     gene            complement(5307..6257)
                     /gene="cbl"
                     /locus_tag="KW011_004054"
     CDS             complement(5307..6257)
                     /gene="cbl"
                     /locus_tag="KW011_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HBH7662262.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT         1811 a         1539 c         1369 g         1652 t
ORIGIN      
        1 tggcgcttga agcacaatct attttctgga tagttttctt ctccataatg ctggcaaaca
       61 ttgcgcatga catggtggtg tgtgtgcaac aaccgatgtt taccgaaatg tttggtgcca
      121 gttatcgcta tagtggcgct ggagtcggtt atcaggttgc cagtgtggtt ggcggtggat
      181 ttacaccttt tattgccgct gcactcatca cttactttgc cgggaactgg catagcgtcg
      241 ccatttattt gctggctgga tgcctgattt ccgcaatgac cgctttgttg atgaaagaca
      301 gtcaacgcgc ttgatagcct ggcgaagatc atccgatctt cgccttacac ttttgtttca
      361 catttctgtg acatactatc ggatgtgcgg taattgtatg gaacaggaga cacacatgaa
      421 taataagggc tccggtctga ccccagctca ggcactggat aaactcgacg cgctgtatga
      481 gcaatctgta gtcgcattac gcaacgccat tggcaactat attacaagtg gcgaattacc
      541 tgatgaaaac gcccgcaaac aaggtctttt tgtctatcca tcactgaccg taacctggga
      601 cggtagcaca accaatcccc ccaaaacgcg cgcatttggt cgttttactc acgcaggcag
      661 ctacaccacc acgattactc gccctactct ctttcgttcg tatcttaatg aacaacttac
      721 gttgctgtat caggattatg gtgcgcatat ctctgtgcaa ccctcgcagc atgaaatccc
      781 ttatccttat gtcatcgatg gctctgaatt gacacttgat cgctcaatga gcgctgggtt
      841 aactcgctac ttcccgacaa cagaactggc gcaaattggc gatgaaactg cagacggcat
      901 ttatcatcca acggaattct ccccgctatc gcattttgat gcgcgccgcg tcgatttttc
      961 cctcgcacgg ttgcgccatt ataccggtac gccagttgaa cattttcagc cgttcgtctt
     1021 gtttaccaac tacacacgtt atgtggatga attcgttcgt tggggatgca gccagatcct
     1081 cgatcctgat agtccttaca ttgccctttc ttgtgctggc gggaactgga tcaccgccga
     1141 aaccgaagcg ccagaagaag ccatttccga ccttgcatgg aaaaaacacc agatgccagc
     1201 atggaattta attaccgccg atgctcaggg tattactctg gtgaatattg gcgtgggacc
     1261 atcaaatgct aaaaccatct gcgatcatct ggcagtgcta cgcccggatg tctggttgat
     1321 gattggtcac tgtggcggat tacgtgaaag tcaggccatt ggcgattatg tacttgcaca
     1381 cgcttattta cgcgatgacc acgttcttga tgcggttctg ccgcccgata ttcctattcc
     1441 gagcattgct gaagtgcaac gtgcgcttta tgacgccacc aagctggtga gcggcaggcc
     1501 cggtgaggaa gtcaaacagc ggctacgtac tggtactgtg gtaaccacag atgacaggaa
     1561 ctgggaatta cgttactcag cttctgcact tcgttttaac ttaagccggg ccgtagcaat
     1621 tgatatggaa agtgcaacca ttgccgcgca aggatatcgt ttccgcgtgc catacgggac
     1681 actactgtgt gtttcagata aaccattaca tggtgagatt aaacttcccg gtcaggctaa
     1741 ccgtttttat gaaggcgcta tttccgaaca tctgcaaatt gacattcggg cgatcgattt
     1801 gctgcgcgca gaaggcgacc gactgcattc gcgtaaatta cgaaccttta atgagccgcc
     1861 gttccgataa taagttctga accttcttat cagaaataga tgtgaaggag taagtaagac
     1921 acctggcaaa tagcctgcaa taatcgtggg ctgtttgctt ccttgggcgg atacgagttt
     1981 tattatcgtc ttaatgattt ccacatatta aaagcaagta tgctttcaaa acacaattat
     2041 aaaaaatccc gccaacaata taagttttta taaaattatg gctttagaat atttttattt
     2101 ctaatagacg agatttttcc tgttatgata taatatgccg aattaacaca tgttaacgat
     2161 ttaccagtaa tgtaaataaa ttttcgagga gatcattcca gtgggacgta aatgggccaa
     2221 tattgttgct aaaaaaacgg ctaaagacgg tgcaacgtct aaaatttatg caaaattcgg
     2281 tgtagaaatc tatgctgctg ctaaacaagg tgaacccgat ccagaattaa acacatcttt
     2341 aaaattcgtt attgaacgtg caaagcaggc acaagttcca aagcacgtta ttgataaagc
     2401 aattgataaa gccaaaggcg gcggagatga aacgttcgtg cagggacgtt atgaaggctt
     2461 tggtcctaat ggctcaatga ttatcgccga gacattgact tcaaatgtta accgtacgat
     2521 tgctaacgtt cgcacaattt tcaataaaaa aggcggcaat atcggagcgg caggttctgt
     2581 cagctatatg tttgacaatg ctggtgtgat tgtatttaaa ggatcagacc ctgaccatat
     2641 ttttgaaatt ttacttgaag ctgaagttga tgttcgtgat gtgactgaag aagaaggtaa
     2701 cattgttatt tataccgaac ctactgacct tcataaagga atcgcggctc taaaagcagc
     2761 tggaatcact gagttctcaa caacagaatt agaaatgatt gctcaatctg aagttgagct
     2821 ttccccagaa gatttagaaa tctttgaagg gcttgttgat gcccttgaag atgacgacga
     2881 tgtacaaaaa gtttatcata acgtcgcaaa tctctaatta tcttttaaag aaatctgtct
     2941 ttacggcaga tttctttaat ctcatataat tcttataaaa aatataagat tcaactcgtc
     3001 atattgatta tacccccccg ttcccagaga aataatattt attaaaattc cagttcttct
     3061 ttttctgatt acagaaggca aagtggcaat tacgcatagt ttcccgataa agacgcgata
     3121 gcgacatccc gcataaggca tttttctctt tatctttgta cggtacttca tggaacagag
     3181 tttttgacct tgcgaatcgt gatgtctgtt ggggaggggc aatttgctca ctgaagcgtg
     3241 agactcgatt aagcgcacga aacacagaaa tcaaaaaacc cggtcacttt tttacaaggt
     3301 aaccgggtaa aaataatttt tattttttaa ctgttttgag actcatagag atgtctcaaa
     3361 actaaaattt ggctcctctg actggactcg aaccagtgac atacggatta acagtccgcc
     3421 gttctaccga ctgaactaca gaggaatcgt gagaacgagg cgaatattag cgatgcccac
     3481 ccacaatgtc aaagcctgtt ttttaaattt gaaatcgttt gctgaaataa tctgcatttt
     3541 gtcgtttatt ccgacacaac tggctttttt tcacactttt gcggttcgga tcgagggtat
     3601 ttccacagcc aacgtccagt aaccattcgc cagtaaaaca gcacggcccg cacagcccag
     3661 tcggcaaaca ttcccatcca gacaccaacc acaccccagc caagcatgat gcccagcaca
     3721 taaccgacga caacccggca accccacatg ctcaacatag aaacccacat ggcgtaacgg
     3781 gcatcgcgag cacctttaaa tccagcgggt agcacccatg aggcggacca aataggcata
     3841 aataaagcat ttagccaaat cagaatcaca acgacatgtt taacctgtgg atcctgggtg
     3901 taaaacgatg ccataacccc ggcaaaggga gccgttagcc aggcgatggc cgttaatcca
     3961 agagtggaaa gccagaacac atgccgcaac tgaatctctg cttgcgctat ctgccctacc
     4021 cccaaccttc ggcctgtaat gatcgtagaa gcagagccga gcgcacttcc gggtaagttg
     4081 ataagagccg caattgaaaa cgcgataaaa tttccggcaa taacactggt ccccatcccg
     4141 gcaacgaaca tttgggttaa taaccgacca ctggtaaata acactgattc gacactcgcg
     4201 ggaataccaa tccccatgac ttcccagata atgctaaaat tcagcggttt aaaatagctc
     4261 tttaacgaaa tccttagcgc aggattaaaa ccaatcgcca gcacccacaa aattgcaact
     4321 gcgccaatat aacgagaaat ggttaaaccc agccctgccc cgacaaatcc cagtcccggc
     4381 caggagaaaa ggccgtaaat caatatgccg ctaataataa tattaagaat attcaggcta
     4441 ccgttaatca atagcggtat tttcgtattc cctgcaccac gaagtgcccc gctaccaata
     4501 agagtgatgg cagctgctgg ataactgagt accgtcagct ccagataagt caacgccagt
     4561 gctttaactt ctgtcgtggc atcacccgcg acgaaatcaa taatttgttc gccaaaatga
     4621 tgaataagcg ttgccaacag tacggcaaac aacgtcatga tcaccaatga ctgccgcgtc
     4681 gccaccctcg ctcgtcgtcg atcccgctta ccgagactaa atgccacaac gacagtagta
     4741 ccaagatcga tagcagcaaa aaaagccata atgaccatat tgaagctgtc cgccaatccc
     4801 acgccggcca tcgcatcttt tcccagccag ctgaccagaa aagtgctcag aacccccatc
     4861 aacaggacac aggcattctc catgaagata ggaacagcaa gcggggttat ctcgcgccag
     4921 aacaacactt tgtagctctt gcgtttagcg tgccagcgag tgccgtgaac aacctggcgt
     4981 aaagcagagg agatattcaa agccgacctt aattgcagaa agtgaaacca catttcaaat
     5041 aatgagggag aatcagcaaa gctgcaaaga ttttcgccaa caaattgtct gcaaatgcaa
     5101 caaactgttg atagaaacgg caaacagttg gggaatttaa aaatcgggtt tgacaaaaga
     5161 tttttcgccg ttaagatgtg cctcaacagc gattcctctg tagttcagtc ggtagaacgg
     5221 cggactgtta atccgtatgt cactggttcg agtccagtca gaggagccaa atttctgaaa
     5281 agcccgcttt tatagcgggc tttttgctat atctgataat caatttcctc ttcactgttt
     5341 tccatcacct gccgcttgat atcctcaact gacagtcctg cattacaaag ttccagaaag
     5401 cgccagacat agttacgctg aagttgtcct cgcttcagtc ccaaccagac agtattagca
     5461 tcgaaaagat gccgcgtatc cagacggatt aaattctctt cctcttgttc gccgctggat
     5521 tgttcggcaa ctaatccgat cccaagccca agagcaacat aggttttaat gacatcagaa
     5581 tcctgcgcac ttaatacaat atctgccagc aaacctttgc gggcaaatgc gtcatcaata
     5641 cgtgagcgcc ccgtaatccc ctgtcggtaa gtgattaacg gccacttcgc tattgattcc
     5701 agcgtcaatg gtgtaatttg cgttaaggga tgatcgagtg gaacaagcaa actatggtgc
     5761 caacgaaacc acgggaaggc gacgagctgc gggtcattac tcaaacgctc gctggcgata
     5821 ccaatatcag cttcgccatt ttgcaacaac gtcgcaattt cctgtggcgt cccctggatt
     5881 agctcgagcc gaacctccgg gaaaagttcg cgaaaagctt taatgacctc tggcaagcta
     5941 taacgtgcct gagtatgcgt cgttgcaata gtgagaacgc cagacgtatc gttggtaaac
     6001 aggtctgcaa gccgacgaac attactggct tcattcagaa tacgttctgc aatgaccagt
     6061 aatgctttgc ccggttcagt catgcccagc agtcgcttac ctcgtcgaac aaatatttcg
     6121 atgccaagtt catcctccag ttcccgaata tgacggctga cgccggactg tgaggtaaaa
     6181 agcatattcg caacctctgt caggttgtaa tcctgacgtg cagcctcgcg gattatcttt
     6241 agttgttgga aattcacggt aaactccggg cagttcagat ttcccgttat tgttaaagtc
     6301 taatgcccgg cataacaaat aataaaaacc cgcatcttat tccatcccga tataacgctt
     6361 agctcaccaa t
//
LOCUS       DAESHI010000181         6319 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.184, whole genome shotgun sequence.
ACCESSION   DAESHI010000181 DAESHI010000000
VERSION     DAESHI010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6319
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.184"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..411
                     /locus_tag="KW011_004055"
     CDS             <1..411
                     /locus_tag="KW011_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBH7662263.1"
                     /translation="VGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLA
                     YTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVD
                     WYNNRRLLERLGHTPPAEAEKAYYASIGNDDLAA"
     gene            457..807
                     /gene="traA"
                     /locus_tag="KW011_004056"
                     /pseudo
     CDS             457..807
                     /gene="traA"
                     /locus_tag="KW011_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
     gene            822..1133
                     /gene="traL"
                     /locus_tag="KW011_004057"
     CDS             822..1133
                     /gene="traL"
                     /locus_tag="KW011_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HBH7662264.1"
                     /translation="MLGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            1155..1337
                     /locus_tag="KW011_004058"
                     /pseudo
     CDS             1155..1337
                     /locus_tag="KW011_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1339..2589
                     /locus_tag="KW011_004059"
                     /pseudo
     CDS             1339..2589
                     /locus_tag="KW011_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            2586..2972
                     /gene="trbI"
                     /locus_tag="KW011_004060"
     CDS             2586..2972
                     /gene="trbI"
                     /locus_tag="KW011_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HBH7662265.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PMTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            2969..4273
                     /locus_tag="KW011_004061"
     CDS             2969..4273
                     /locus_tag="KW011_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HBH7662266.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     RVAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVFEPKPDREVMNAE
                     RLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
                     IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDN
                     PDSGVVVSIAGEGRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEA
                     IRRETERRADEIVRKMAENKPDLPDGKTELAVREIAGQERDRAAITEREAALPESVLR
                     ESQREREAVREVARENLLQERLQQMERDMVRDLQKEKNPGGD"
     gene            4293..5040
                     /gene="traX"
                     /locus_tag="KW011_004062"
                     /pseudo
     CDS             4293..5040
                     /gene="traX"
                     /locus_tag="KW011_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            5095..5655
                     /locus_tag="KW011_004063"
     CDS             5095..5655
                     /locus_tag="KW011_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HBH7662267.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            5785..5997
                     /locus_tag="KW011_004064"
     CDS             5785..5997
                     /locus_tag="KW011_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HBH7662268.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT         1503 a         1530 c         1928 g         1358 t
ORIGIN      
        1 gtggggtggc gggtctcatc gtccatggag acgacattcg tgctggatgc actggagcag
       61 gcgttatggg cccgtcgacc gtccggcacg gtccatcaca gtgataaagg ttctcagtat
      121 gtatcgctgg cctacacaca gcggcttaag gaagccggat tactggcatc aacaggaagt
      181 acaggcgact cgtatgacaa cgcgatggcg gagagcatca atggtcttta caaagcggag
      241 gtaatacacc gtaagagctg gaaaaaccgt gcagaagtgg aactggccac actcacgtgg
      301 gtggactggt ataacaatcg acgattgctg gaaaggctgg gccatactcc tccggcagaa
      361 gcagaaaaag cttattatgc ttccatcgga aacgatgatc tggcagcctg agttcacaga
      421 taaaacactc tccaggaaac ccggggcggt tcagcgttaa gtgttcaggg tacttctgcg
      481 cccgtcaaaa agaaatcgtt tttttctaaa ttcactcgtc tgaatatgct tcgcctggtt
      541 cgtgcagtga tcccggttgc tgttctgatg atgttgttcc ctgagttagc tatggctgct
      601 ggtaaaggca cggacctgat ggctaaaggt caggaaacgg ttaaggcgac cttcggtaag
      661 gactccagta tcgttaaatg ggttgttctg gctgaagttc tggtcggtgc agtcatgtac
      721 atgatgacca aaaacgtcaa gttcctggtc ggctttgcca tactctctgt atttattgca
      781 gtaggtatgt ctgttgccgg attctgacag gaaataaaac gatgttggga gacgagaata
      841 aacttaagaa atatcgtttc ccggaaacac tgaccaacca gagccgctgg tttggcctgc
      901 cactggatga actgatcccc gcagcaatct gtgttggctg gggtatcaca acatcgaaat
      961 atctgttcgg tattggtgca gcggttctgg tttatttcgg gattaaaaaa ctgaaaaaag
     1021 ggcggggcag ttcctggtta cgtgacctga tttactggta tatgccaaca gccctgctgc
     1081 gcggtatttt tcataatgtt cccgattcgt gtttccggca gtggattaaa tagaactgat
     1141 accaggattg ttatatggaa cacggtgccc gtttaagtac cagtcgtgta atggccatcg
     1201 cctttatatt tatgtcagtg cttattgttc tcagcctctc tgttaacctc attcaggggg
     1261 tgaataacta ccgtcttcag aatgagcaac gcactgccgt gaccccaatg ggatttaatg
     1321 tgccctttgc cgtgtcacac ctaccgtaac cagctggcgt ttatcgatat tttcttccgg
     1381 ggaatgaata acaccaacta caacatggcg gtctgtggca cctccggggc cggtaaaacc
     1441 gggctgatac agccacttat ccgcagcgtg ctggactccg ggggctttgc cgtggtgttc
     1501 gacatggggg atggctacaa gtccctgtgt gagaacatgg ggggcgtata tctggacggt
     1561 gaaaccctgc gttttaaccc gtttgcgaac atcaccgata tcgaccagtc agcggagcgt
     1621 gtccgtgacc agttgtcagt gatggccagc cccaacggta acctggatga agtgcatgaa
     1681 ggtctgctgc tgcaggcggt cagggcctcc tggctggcca aagagaacag ggcacgtatt
     1741 gatgacgtgg tggatttcct gaaaaacgcc agtgacagcg agcagtatgc cgggtcaccg
     1801 actatccgca gccgtctgga cgaaatgatt gtgctgcttg accagtacac tgccaacggc
     1861 acttacggcc agtattttaa ctctgatgag ccgtccctgc gggatgacgc aaaaatggtg
     1921 gtgctggagc tgggcggact ggaagaccgt ccgtcactgc tggttgcggt gatgttctcc
     1981 ctgattatct acatcgagaa caggatgtac cgcacgccgc gtaacctcaa gaaactgaac
     2041 gtcattgatg aaggctggcg tctgctggac ttcaaaaatc acaaggtcgg tgagtttatt
     2101 gagaaaggtt accgtaccgc ccgtcgtcat accggtgctt atatcaccat cacacagaac
     2161 atcgtcgact ttgactctga caaggcctcc agtgcggccc gtgcggcatg gggtaactcc
     2221 tcctacaaaa ttatcctcaa acagagtgcg aaggagttcg caaaatacaa ccagctgtat
     2281 ccggaccagt tccagccact gcagcgcgac atgattggta agtttggtgc ggccaaagac
     2341 cagtggttca gttccttcct gctgcaggtg gaaaaccatt cctcctggca ccgcctgttt
     2401 gtggacccgt taagccgcgc catgtacagc tctgacggcc cggattttga gtttgtgcag
     2461 cagaaacgta aagaggggct gagtattcat gaggcggtgt ggcagctggc gtggaagaag
     2521 tcagggccgg agatggcttc gctggaagcc tggctggaag aacatgagaa atacaggagt
     2581 gttgcatgac cacaacgcaa aaaacgactg atgttacagc cccccgacgc agccactggt
     2641 ggtggacggt gccgggatgc ctggcaatgg tgttactgaa cgcagccgtc agttacggca
     2701 ttgtcagact gaacgcaccg atgactgccg ccttcaacat gaaacagact gtggatgcat
     2761 tttttgacag cgccagtcag aaacaactgt cagaagcaca gtcaaaagcc ctttcagcgc
     2821 gttttaacac ggcactggaa gccagcctgc aggcgtggca gcagaaacac cacgcggtca
     2881 ttctggtgtc gcctgccgtg gtacaggggg ctccggatat cacccgggaa atccagcagg
     2941 atattgcccg gcgaatgaga gcggaaccat gagatgccgg gggctgattg ccctgctgat
     3001 atgggggcag agtgtggccg ccgccgatct tggcacctgg ggtgatctgt ggccggtaaa
     3061 ggagccggac atgctgacgg tgatcatgca gcgcctgacg gcactggagc agtccggtga
     3121 gatgggccgg aaaatggatg cctttaagga gcgggtgatc cgcaacagtc tgcggccccc
     3181 tgccgtgccc ggtatcggac gcacggagaa atacagcagc cggttgtttg acccgtccgt
     3241 cagactggct gcggatatcc gggataacga agggcgggtg gccggctttg agtcagccta
     3301 cgtggccctg tcgcgtatga agcagcatgt gcaggtgtac accgataacc gtcagggctg
     3361 gacggatgcc atcaacaatg ccgtacagaa aggaactgcc cacgatgtgt ttgagccgaa
     3421 accggaccgg gaggtcatga atgcagagcg gctgttcagt acggcgcggg agctgcggga
     3481 cgtggcggca ggccgtgccg ttctccgtca ggcggggctg gccgggggag acagtcctgc
     3541 acggtttatt gctccgggac gtaaatatcc gcaaccgtat gtggcactgc cggcgtttga
     3601 ccgtaacggc aagtccgccg gtatctggct gaacccgctg accacggatg acggaaacgg
     3661 gctgcgggga ttcagcggtg aaggtcgggt aaaaggcagc ggggatgcgc agttcgtggc
     3721 cctgcagggc agccgtaacg gagaaagcct gctggctgat aatatgcagg atggcgtccg
     3781 gattgcccgt gataatcctg acagtggtgt ggtggtaagc attgccggtg aaggccgtcc
     3841 gtggaatccc ggtgccatca ccggtggccg cgtgtggggg gatatcccgg acaacagcgt
     3901 ccagccggga gccggaaatg gcgaaccggt cacggcagag gtactggcac agcggcaggc
     3961 tgaagaggcc atccgccgtg aaacggaacg tcgcgcagat gaaattgtcc gtaaaatggc
     4021 agagaacaaa cctgacctgc cggacggcaa aacagagctg gctgtcaggg aaattgccgg
     4081 gcaggagcgt gaccgggctg ccataactga acgggaagcc gcgctgccgg agagtgtgct
     4141 gcgtgaatca caacgggagc gggaagcggt ccgggaggtt gcccgggaaa atctgctgca
     4201 ggagcgtctg cagcagatgg agcgggatat ggttcgtgac ctgcagaaag agaaaaaccc
     4261 gggcggagac tgatacagga agacaaacac tgatgacaac ggataacacg aacacgacac
     4321 gtaacgattc actggctgca cggaccgata cctggttgca gtcatttctg gtctggtcac
     4381 ccggacagcg ggatatcatc aaaacggtgg cactggtgct gatggtactg gaccacatca
     4441 atctgatatt ccagctgaag caggaatgga tgtttctggc cgggcgagga gcctttccgc
     4501 tttttgccct ggtgtggggg ctgaatctgt cccgtcatgc gcatatccgg caaccggcca
     4561 ttaaccggct gtggggatgg ggaattattg cccagttcgc atattatctg gcaggctttc
     4621 cctggtatga ggggaatatc ctgtttgcct ttgcagtggc ggcacaggtg ctgacgtggt
     4681 gtgaaacgcg cagtgggtgg cgtacagcag cggccattct tctgatggcc ctgttggggg
     4741 cctttgtccg gcaccagtta cggcattgcc gggctgctga tgctggcagt cagccaccga
     4801 ctgtaccggg cggaagacag agcggaacgt ctggcgctgg tcgcctgcct gctggctgtt
     4861 attccggcgc ttaaccttgc caccagtgat gcagcggcgg tggccggtct tgtgatgacg
     4921 gtgctgaccg ttggtctggt gtcgtgtgca ggaaaatcat taccccgttt ctggcccggg
     4981 gattttttcc cggtattcta cgcctgtcat ctggctgtgc tgggcgttct ggcgctgtga
     5041 cgggtgtggt atctttgacc gtaacaggat gattcgtcag aggcagaaca cagcatgaca
     5101 gagcagaagc gaccggtact gacactgaag cggaaaacgg aaggggagac gcctgtccgc
     5161 agccggaaaa ccatcatcaa tgtcaccacg ccaccaaagt ggaaggtgaa aaagcagaag
     5221 ctggccgaga aagcagcccg ggaagcagag ctggcggcaa aaaaagcgca ggccagacag
     5281 gcactgtcca tttatctgaa cctgcccacg ctggatgagg ccgtgaatac cctgaaaccc
     5341 tggtggccgg gattatttga cggtgacacc ccccggcttc tggcctgcgg tatccgggac
     5401 gtgttactgg aagacgtggc gcagcggaat atcccgctct cgcataaaaa actgcgcagg
     5461 gcgctgaagg ccatcacccg ttcagaaagc tatctgtgtg ccatgaaagc cggtgcctgc
     5521 cggtatgaca cggaagggta tgtgacggag catatttctc aggaggagga agcgtatgcg
     5581 gcagcgcgtc tggataaaat ccgccgccag aaccggataa aggcagaact tcaggccgtg
     5641 cttgatgaga aataaaaaaa agcctccatc agagaggagg cagggaaata aggcttaaaa
     5701 ggaaataact tccgcttccg aaaggaagcc agtccactga attatttatc acaataatac
     5761 tgacaactaa agggggacaa gaaaatgaat ggattcagaa acagttcacg gaacggccag
     5821 gtctggcgtt accagcgtgc cggcgggcgg gccgttattc tggaagtcag tggacgctgg
     5881 atggaagcgg cagaagcatg gcgacgggct gcctgtgttg cgcccaggac agactggcag
     5941 cagtttgccc gaaaaagggc tgaacactgc caccggcgct gccggggaag ggtgtaacga
     6001 aaaaagcagc cttcattact ccaggagtca gaaggctgaa aggagtttat gagatttgct
     6061 ttttttaata tccggtcggg cagtatgacc ggacagttct gagtagcaca gagttttttc
     6121 cgtattaagt cgtgatttta tattttgtga ttaatttcac aaaataaggt gttattcagt
     6181 gtgtgctgca atattcagga tgcctgaaat acagatgcta tttatctgat atggagaata
     6241 acatgaggaa atagtaaggc agtatgactt tccgtgagac agatgctggc caacgtggga
     6301 tatttaacgc gggttaaca
//
LOCUS       DAESHI010000182         6203 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.185, whole genome shotgun sequence.
ACCESSION   DAESHI010000182 DAESHI010000000
VERSION     DAESHI010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.185"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            12..754
                     /locus_tag="KW011_004065"
                     /pseudo
     CDS             12..754
                     /locus_tag="KW011_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(796..1248)
                     /locus_tag="KW011_004066"
                     /pseudo
     CDS             complement(796..1248)
                     /locus_tag="KW011_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1312..1458
                     /locus_tag="KW011_004067"
                     /pseudo
     CDS             1312..1458
                     /locus_tag="KW011_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1458..2615)
                     /gene="intS"
                     /locus_tag="KW011_004068"
     CDS             complement(1458..2615)
                     /gene="intS"
                     /locus_tag="KW011_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HBH7662269.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2777..2851)
                     /locus_tag="KW011_004069"
     tRNA            complement(2777..2851)
                     /locus_tag="KW011_004069"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2816..2818),aa:Arg,seq:cct)
     gene            complement(2927..3859)
                     /locus_tag="KW011_004070"
     CDS             complement(2927..3859)
                     /locus_tag="KW011_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBH7662270.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            4153..4908
                     /gene="mlaA"
                     /locus_tag="KW011_004071"
     CDS             4153..4908
                     /gene="mlaA"
                     /locus_tag="KW011_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HBH7662271.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(5090..5425)
                     /locus_tag="KW011_004072"
     CDS             complement(5090..5425)
                     /locus_tag="KW011_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662272.1"
                     /translation="MAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDD
                     AKFRRDNVKEIISNLFANKVDVDHPENKYKDFKDLEDNVEKRLQNRQAKYQNEINQLS
                     APGVNFDDI"
     gene            complement(5383..5775)
                     /locus_tag="KW011_004073"
     CDS             complement(5383..5775)
                     /locus_tag="KW011_004073"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662273.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLF
                     TWYGKDKKGNSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSEKARN
                     ASAMLQRGLKAVKLSWQNHLQQVPIIRL"
     gene            complement(5782..6158)
                     /locus_tag="KW011_004074"
                     /pseudo
     CDS             complement(5782..6158)
                     /locus_tag="KW011_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937824.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1546 a         1369 c         1374 g         1914 t
ORIGIN      
        1 gggtggatga tatggcaact acatgttcag ttatattgat tttggagtcc tttgatgttt
       61 atttcggaaa agagagtgtg tttctggaga gaggttcatc tgtacttgtc gactctagct
      121 ctagagattt tttcctgaca tatcctgaaa gagtgatagt ggcggatttt ggcgctgagt
      181 ttattagtcg ctatttgaaa gctaataact taagggatat ttctgattgt agggaatatc
      241 catcttattt aaaaataaac tttgctgact tcagtttaat taaaggatta attagttggg
      301 ctaatcactg tgctgaatac atagaaattt ttgatgagtc tattgctttt acatgtctct
      361 ctgcattttc ttctgaaaaa caatttggag tatttctgtt tggatgtttg aaaagcacag
      421 ggctaaagtt aaaacgatta ttcatacgga tttatctgca ccatggcgtc ttaaggatat
      481 atcatcaaga ttatatctca gcgaaagttt actaaagatg aaattgaaag aagagggggt
      541 atcattcagt aagatcatac ttgatgagag gatgcaaatg gctgaatatt tactcagcac
      601 tcgttgttat cctattagta aagtagctaa ggtctgtggt tatgccagtg tctcatactt
      661 tacttatgta tttagacgtt attttggtgt ttctccaagt caatactctc agaggagttc
      721 agaaagtaaa attcttactc accagggaat ctgatcattg ttcttgcccc cttatttcca
      781 gacagggggt gtatcttaag ttaacgttac ccgctgacgt cgatattctc gcggagagcg
      841 ataacccagc gcactgtgcg gatggttttc attgtaatgt tcgatcgcca ctgcaagatt
      901 atgcaatgcc gttcttacat tcggtttcgg catgaacgcg atgtagtctt ccttcatcgt
      961 tttcacgaac ctttctgcca ttccattact ctgaggactg ctgattgccg ttgtgcaggg
     1021 ctccagattc aactctctgg cgaactgccg tgtttcatgc gctctatacg ctgaaccgtt
     1081 atctgtcagc cactggatgg actgttccgg tactttatcg ccaaagcgtt tttctatcgc
     1141 tcctaacatg acatcctgca ctgtcgcttt atcgtaacct cccgtgcttg cggcccagtc
     1201 tatggcttca cgatcgcaac agtccagggc gaacgtaaca cgcagttttg aatcgccacg
     1261 ggtttaacag acacctcaga gtcatttaag atggcttaaa gagaggtgcc catgagcggt
     1321 aagcgttatc ccgaagagtt taaaactgaa gcagtcaaac aggttgttga tcgcggttat
     1381 tctgttgcca gcgttgcaac acgtcttgat atcactaccc acagccttta tgcctggata
     1441 aagaagtacg gtccggatta ctccactttt tcatcaagcc aatccgccca ccactgcatc
     1501 atttctctgc gcttatcgag atactgagca tggttgtaaa taccgcgcac agatccgccg
     1561 ttggcatgtg ccagctgcac ttcaatagcg tcagcaggcc attcgtgctc gttcataatc
     1621 gtgctgaatt catgcctgaa tccgtgaccg ctttccagac cttcatagcc gatttgtttg
     1681 atcacaagca ataccgcgtt ctcgcagatt ggcttcttct tatcgttgcg cccggcaaaa
     1741 acaaactctg agactggttt ggtgattgag cttagcgtag tgagaagttc aaccacctgg
     1801 tctgacatcg ggaccacatg aattttgcgt cccttcatca cactggcgtc gatggtgata
     1861 atcctgtttt caaaatcgac gttcttccat agcatggaac gaagctcttt tgttcttagg
     1921 gctgtgtagc gtaaaacttt ggtcgcaatg agcgatacga tacttcctga aaatgttgcc
     1981 agtgctttgt tgaatgcagg catctggtct gctggaagga acgggaagtt cttcttgcgg
     2041 tatcctttca tggcgtctgc aaggtcaggt gccgggttat atttagccct tccggtgaca
     2101 atagcgtaac gaaaaacctc gccgcatctt ctgcgggctt tgttggctcg ctccattgca
     2161 ccgcgatctt caaatctgcg gattacttcc agcagttgca tcggctcaat atcctgaatc
     2221 tcaagaccgc cgatgatggg taaaatgtcg tcatcaaaca ttttggcaag ttcagttgca
     2281 tagcctactg accagacttg cttcttgtgc tcgtaccatt ccttgtaaat cgcactaaat
     2341 gaattgttgt tagacgaagc ctttttcgcc tttaccggaa cgatgccaac cgagatgtct
     2401 ttcctcgcag tccatgcctt atcccttgct tcctgcaaag tcataagcgg atattttccg
     2461 acggtcagga ttttctcctt accgtcaatc ttgtagcgaa gctgccatac ctttttcccg
     2521 gatacaggaa cataaaggta caggccatta ccatcgagaa ggcggtatgg tttttctttc
     2581 ggctttgctg cttcaatctg cttaacggtg agcatgggta aaaatccggt gggtaaaatt
     2641 attttatcca ctttttaccc gtcatggagt gcggctgtca acgatctgac gcgaaccatg
     2701 acgaaccgtg aatatacgga aggcttgata ttcaggggat tttgcggact ggtacggatg
     2761 ggagcgaact gataaatggt gtcccctgca ggaatcgaac ctgcaattag cccttaggag
     2821 gggctcgtta tatccattta actaagagga caatgcggca tgagtatacc cgctaatgga
     2881 gtgcggggta agtacgctgc cgcttgattg cttaaaccct cgccatttat gccgggtttt
     2941 tatcattttt cttaatgttt tccgcacgtt ctgctttttg gcgtgcttct gctttacgct
     3001 tgttgctcat gtcgttacga atctgtgcat gactcattaa cgcgaagata aaggtgccgc
     3061 cgcagatgtt ccccgctaaa gtaggtagtg cgaagggcca gatgaaatcg ctccagtgca
     3121 gcgtaccgtt aaacaccaga tagaggattt caacagaacc gaccacgata tgggtggtgt
     3181 cacctagggc aataagccag gtcatcaata taatcaccac aatctttgcc gcacctgctg
     3241 caggaaacat ccataccata gtggcgatca gccagccgga aatgatcgcg ttggcaaaca
     3301 tctcgctggg ggtgttcttc atcacatcca tgccgatttt gacaaatgca tcgcgagttt
     3361 cttcattgaa gataggcata tattcaaatg cccacgcagc aataccagtc ccgagaatat
     3421 tacccagcag cacgactccc cataaccgca taagtaagcc aacgttgctc attgtcggtt
     3481 tttgcatgac gggtagtacc gcagtcacgg tgttttcggt aaataattgc tggcgggcca
     3541 ttatgacgat aataaaacca aaggtataac cgagattctc cagtaagaag ctgcccggca
     3601 caccttccag ttcgacatga aatatccctt ttgccagtaa cgaagcgccc atcgacagac
     3661 ccgccgcaat ggctgaccac agtagcgcca ttgcgtcgcg ttccagctct ttttcaccat
     3721 cctggcggat atgctcatga attgccatcg cccgggaggg gagtcggtct tcatctattt
     3781 ctattttttt gccgcgctct ttttcttcgc tctcaacttc aatttcgtcg ctgtgttgat
     3841 caattttgtc gttgtccatg gtctcttctc ttgaattagc acgtatagct aaagcgtagc
     3901 ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat gtagaaaagg ctgcgtttgc
     3961 ctttttctgt ttctatagaa tcaagtagcc tacagggcgg cgattaccag gctatgatca
     4021 aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct gttacaatcg cccacaccta
     4081 aacaggcgga tacggtatcg ttccgtcatg gatggcaaac tgcataagcc ataaaaaaac
     4141 agggagacat ttatgaagct tcgcctgtcg gcgcttgctc tgggaactac gcttctggtg
     4201 gggtgtgcga gttccggtac agatcagcaa gggcgttctg acccgttaga agggttcaac
     4261 cgcaccatgt acaacttcaa cttcaacgta ttagacccgt atattgttcg accggtcgct
     4321 gtcgcctggc gtgattatgt tccgcaaccg gcgcgtaatg gtttgagcaa ctttactggc
     4381 aaccttgaag aacctgcggt gatggttaac tacttcttgc agggcgaccc ttatcagggg
     4441 atggtccact ttacccgctt tttcctgaac accattttgg ggatgggcgg ctttattgat
     4501 gttgcaggga tggcgaaccc gaaactgcaa cggactgaac ctcaccgctt cggtagtacg
     4561 cttggtcatt atggcgtggg ttatgggcct tacgttcagt taccgttcta cggtagcttc
     4621 acgctgcgtg atgacggtgg tgatatggcg gatggtcttt acccggttct ttcctggctg
     4681 acctggccga tgtctgtggg taaatggacg cttgaaggga tcgaaacccg cgctcagctg
     4741 ctggattccg atggtctgct gcgtcagtcg tccgatcctt atattatggt gcgcgaagcg
     4801 tacttccagc gtcatgattt catcgctaat ggcggcgaac tcaaaccgca ggaaaatccg
     4861 aacgcacaag cgattcagga tgatttaaaa gatattgatt ctgaataaga aacaaataaa
     4921 aaaggtgagt cgcaaaactc accttttttg ttgttatccc tcaattacca caagtaaaag
     4981 gagataaacc gacaatgacc cggacgtaat gatgtaaaaa tactgatgtt cgtcactgac
     5041 tatttccgat attcaggcgt tgaaaaatca aaaaactgga aaaataattt taaatatcat
     5101 caaaattaac acctggcgca gatagttgat taatttcatt ttgatatttc gcctggcgat
     5161 tttgaagtct tttttcaacg ttatcttcga gatccttaaa gtctttgtat ttattttcag
     5221 gatggtcaac gtcaactttg ttagcgaata aattagaaat tatttctttt acattgtcgc
     5281 gtctaaattt tgcatcgtcc acttgaaaat tatatcttat tgctcgacct aaacgtttaa
     5341 aaacttcgtt aggttctctc agtctttccc cgttgagctc gtttacaacc tgatgatcgg
     5401 aacctgttgc aaatgattct gccatgatag ctttactgct ttcaaaccac gctgaagcat
     5461 tgctgaagca ttcctggctt tttcactaca atattgggtg agactatcac cattaatacg
     5521 aattcctctt tgaatgtggt agtcgtcctg atcgtagtaa ccgagagata agtaatcatt
     5581 tccgttttta tttttaatta caatagcgag agagttgcct tttttatctt taccgtacca
     5641 ggtaaataag tccgttggtt cgctattccc gctctcatca aattccgtta ccgtatgtct
     5701 gagtttgtgt gatatcttat cgaagataaa accaggatct ccatcatata tttcttttaa
     5761 tgcgctgaag atcatttcct tgttattgat atcatgagtc ttctcaacta tgttgtcatt
     5821 aatgatagta cggacgagtt ctttgtcttt atagttcgca ggtaattcta ttggttcttg
     5881 gttgtcgata ccaatagtaa tatggtctgt atcattggag ccaatatcaa tataagtaac
     5941 tgaggaatta ttaatattaa atcttaagga tcgtcgttta ctcgtatggc gttttttata
     6001 agctcaagta gatgtttaat acaggtgctt attccttcct taccattaaa aaggtttttg
     6061 agctcttgta ctaatccatt aacttcatca ttgttatttt cattaatgga ttctgggtaa
     6121 gttgcgctgg tattgttgat tgaaatagat ggtagcatga ttttattcca ttttgattga
     6181 tttgttacaa atatatatta cct
//
LOCUS       DAESHI010000183         6135 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.186, whole genome shotgun sequence.
ACCESSION   DAESHI010000183 DAESHI010000000
VERSION     DAESHI010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.186"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..1255)
                     /locus_tag="KW011_004075"
     CDS             complement(<1..1255)
                     /locus_tag="KW011_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBH7662274.1"
                     /translation="MAQFKVDDFLIELSFNSQKVLKGLEKAEKQTMQVASRIEKRLNK
                     SFRVNPTPLNDSLKLMERNVDKTVSKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQPRI
                     SGSRAITAAYSANMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNETFNRELAKLNQSL
                     RETLSKFNKTTSKNNHSENASNSLDVLATSAIKAGTAIYSFQTALEAYRRIMEVGLKK
                     EASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETLSSFAKFSAGAGDMNADQKES
                     LFSNVIGTSRLMGLSTDEINGILKAFEQMASKGKIQAEELRGQLGDRMAGAFQLFARS
                     LGMTTEELDKAMKDGKILSKDVLPKVSAEMGRMIDKAGGWEKIINSTQTQLGRLSNSW
                     NNNLALMFDGSQEGLTDFTRSLTNLL"
     gene            complement(1412..1621)
                     /locus_tag="KW011_004076"
     CDS             complement(1412..1621)
                     /locus_tag="KW011_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662275.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(1644..2354)
                     /locus_tag="KW011_004077"
     CDS             complement(1644..2354)
                     /locus_tag="KW011_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH7662276.1"
                     /translation="MTNDLYINGKKVDSFGDWSPQGEHPLHTIQRQEHDKRIELEQEL
                     KMSPKVISFVSPEPQEMPDICKTEALFELEREYYPALKSQRLRLDVAYDAVKNFEETS
                     KPSEYDIAYEIKSNPFIYYEGNFNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIAL
                     MADIEQLTKISQKYSKAV"
     gene            complement(2870..3193)
                     /locus_tag="KW011_004078"
     CDS             complement(2870..3193)
                     /locus_tag="KW011_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662277.1"
                     /translation="MNEYELQIFIDSDTAMMVQAFADSGVSIDLDKLLKLMAENSEEI
                     EDFIQEIETGEPRFMFPVTDSNMKRLIIEETNKYSVSPEKYLKAAIAILYSDNILVTD
                     SKVVH"
     gene            complement(3372..3572)
                     /locus_tag="KW011_004079"
     CDS             complement(3372..3572)
                     /locus_tag="KW011_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662278.1"
                     /translation="MLCIKFEYLTDKMIKHVSDLLIKEGGFGDACNPKDIFIHATSPN
                     ATLKTAVTAEWFERNKAELGYW"
     gene            complement(3572..3844)
                     /locus_tag="KW011_004080"
     CDS             complement(3572..3844)
                     /locus_tag="KW011_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBH7662279.1"
                     /translation="MKQWRDDIKRFFATYFMINYETGMLEWIDGGEVKTRDDAYGNPM
                     IRYGTRDYYLKYVIWFLHHEVQATKKIIFRDGNKRNCHPNNLLLEI"
     gene            complement(3847..4125)
                     /locus_tag="KW011_004081"
     CDS             complement(3847..4125)
                     /locus_tag="KW011_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662280.1"
                     /translation="MNKHTLTRVTKTAFDELNRIKNITGLPHSAVMKLAINRDINEQD
                     LLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLSQEV"
     gene            complement(4126..4683)
                     /locus_tag="KW011_004082"
     CDS             complement(4126..4683)
                     /locus_tag="KW011_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662281.1"
                     /translation="MQYSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPSKLDDVFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASTVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEDF"
BASE COUNT         1746 a         1276 c         1070 g         2043 t
ORIGIN      
        1 taagtaaatt agtcagtgac cttgtaaagt ctgttaaacc ttcctgtgaa ccatcgaaca
       61 ttaaggccag attattattc catgaattac ttaagcggcc taactgcgtc tgagtagagt
      121 taataatctt ttcccatcca ccagctttat caatcatcct tcccatttca gcggatactt
      181 taggaagaac atctttagaa agaatcttac cgtctttcat ggccttgtct aattcttccg
      241 tagtcatccc aagacttctg gcaaataact gaaaagcacc tgccattctg tcacctaatt
      301 gaccgcgtag ctcttcagcc tgaatctttc ctttagatgc catctgctcg aaagctttta
      361 aaatcccgtt aatttcatcg gtgcttagcc ccattaatct ggatgtacca atcacattag
      421 aaaataagga ttctttctgg tctgcattca tatcaccagc accagcggaa aacttagcaa
      481 aactgcttaa tgtttcaatc tggtctacac cactactatc agcaagattc ttaacaaatt
      541 ctgtagctcg tttagcacct tcatcaccta aaacgaattg tgccgctcgt tgtgatgctt
      601 cttttttcag accaacctcc ataatccgcc tgtaagcttc taaggctgtc tgaaaactat
      661 aaatggctgt acctgctttg attgcactcg tagctaatac atccagacta tttgaagcat
      721 tttcactgtg attatttttg ctggtggtct tgttaaactt agaaagtgtt tcacgaagag
      781 actgatttaa ctttgctaac tcacgattaa aagtttcgtt tccaagtgtc ttggatttac
      841 tgcttaagct gtaatactgg cttttaaaat acttctcaag cataggatta aagtcacgaa
      901 gcttgctcat attcgctgag taagctgctg tgattgctct gcttccgcta attcgtggct
      961 gtcttggttg tctaagtggc tttaaagtat cctcaatttc agttttaatc ttaaacgctc
     1021 tagtgttttt aagacgctgc tcaatcttgg aaacagtttt atcgacattt ctttccatta
     1081 gtttgagaga atcatttaaa ggcgtaggat taactctaaa cgacttattc aatcgcttct
     1141 caattcgtga tgctacctgc atcgtttgtt tctcggcttt ttccaagcct tttaaaactt
     1201 tctgagagtt aaaacttaac tcaatcagaa aatcatcaac tttaaattgt gccattcata
     1261 tttttcacct ctttaagcgt gttgtacgta aattctattt ttcagtgtgg gtattagctt
     1321 tacagatttc acgtagcaca catacaaaaa aagggagagc taccgatgtc actcgataat
     1381 ctctccccgt atttacccaa ccaattcttt tttaatgtcg gacaagataa tgtaattccg
     1441 tttcacctat gatttgagca tcagaaggaa tacggtcaat acaccacttt ggaatcttgc
     1501 gtgtttttgg tttgtgtgag gtgataactt gacgtttgtt tttaattctt aaggctggct
     1561 tagtttgtaa ctttaagttt tcacaaacag accgaatagt ctctaaacgt tcttcaaaca
     1621 ttaatcacct ctttaattca taatcaaaca gccttgctat acttctgaga aatcttcgtt
     1681 aactgctcaa tatcagccat caaagcaatc ttcttctgcg actcatattc actaacaagt
     1741 tctttcatag tatcaagttc ttttttaagc ttttgcttta acttatccag tttaccggaa
     1801 taatgcttag tcaaattagc ttcagctaac tgaagaatct cttcatctgt tttatcagtc
     1861 atcagaataa attgaccaga accccttaat gcttgtctga catcaattaa ccggaaccct
     1921 tcagggatac tttgaagaac tttaggaacg tcttcaatcg ctgtaccaaa accatcatta
     1981 aagtttcctt cataatagat aaacgggttt gacttaatct cgtaagcaat atcatattct
     2041 gaaggtttcg aggtttcttc gaagttctta acagcatcgt aagcgacatc aagtctaaga
     2101 cgctgtgatt ttaaagctgg gtaatattca cgctcaagct caaatagtgc ttcagttttg
     2161 cagatatcag gcatttcttg aggctcagga gacacaaagg aaattacctt tggtgacatc
     2221 tttagctctt gctcaagctc aatgcgcttg tcatgctctt gacgctgaat agtatgtagc
     2281 ggatgttcac cctgtggtga ccagtctccg aagctatcaa cctttttacc gttaatatat
     2341 aaatcgtttg tcattataat ttcctctaag tgtggttatt tcttaataga gcaccttcaa
     2401 cgccaataca cttagaagat gacttaacga tttattaaag atgctctatt aagaaaagcc
     2461 ctaaacataa tctgtgaagg gctttggagg tttgcttata acgttaacga agtttcagga
     2521 aaactttctt taaaagctct gtttaaaaac tcttaaagaa agccctaact catgattagg
     2581 gcaaagctgt aaaggattca ataatgaatg aacaaagact ttttgagata gcctctcaga
     2641 ccaacctgaa aggctaggac gaaaagcatt gcacctttcc aactcagaat atccatccat
     2701 cggattattg aaagaaaaaa taagagagaa ttgctctccc ccatatatac aattatgtca
     2761 tgctcaaggt tgtttgtcaa cagcttttaa cagattggta acatttatac tttaatagat
     2821 gcacctgcaa tcataccaat caatacaaaa cagataatca ccaattccat taatgcacca
     2881 ccttcgaatc cgtcaccaga atattatcgc tatacaaaat tgctatagcc gccttgagat
     2941 atttttcagg gctaacgctg tatttgttcg tttcttcgat aataagacgc ttcatgtttg
     3001 agtcggtaac aggaaacata aaacgcggtt caccagtttc aatttcctga ataaagtctt
     3061 caatttcttc tgagttttca gccattagct ttagtaattt atcaaggtca attgatacac
     3121 cggaatcagc gaaagcctgc accatcatag cagtgtcgct atcaatgaaa atctgtaatt
     3181 catattcgtt catttttaaa atctcctttt taccagacct cctcaatcgc tctgtaacga
     3241 gctaaacagt ctttttcagg ggtagcctgt acagtggtat tacccaccct aaaataagat
     3301 agttttttgc gttagaatgc gattgagagc gtctgataag ctgtttttct aatttctctt
     3361 aaagtctcgg actaccaata acctaattcc gctttatttc gttcaaacca ttccgctgta
     3421 actgctgtct ttagtgttgc gtttgggctg gtggcatgaa tgaagatatc ttttggattg
     3481 caagcatcac cgaaaccacc ttctttgatg agcaggtctg atacatgctt aatcatttta
     3541 tctgtcaaat attcgaattt aatacataac attaaatctc caggagtaaa ttgtttgggt
     3601 ggcaatttct tttattacca tccctgaaga taatcttttt agttgcctga acttcatgat
     3661 gcaaaaacca gatgacatac ttcagataat aatcacgagt gccataacga atcattggat
     3721 taccgtaagc atcatctctt gtcttaacct ctccaccatc aatccattca agcattcctg
     3781 tctcataatt aatcatgaag taagttgcaa agaaccgttt aatatcatct cgccattgct
     3841 tcataatcag acctcctgag acagtttgtg aattttgata cttagtaacc ttgctaaatc
     3901 cagtccaaaa gcatttaagg atttcagact atcaaggtca tcttgcttaa tcctgatatg
     3961 gtgtcttgca gcctctaagc gactattaag caaatcctgt tcgttaatat cacggttaat
     4021 tgcaagcttc atcactgctg agtgtggcag acctgtaatg tttttgattc ggttcagttc
     4081 atcaaaggct gtctttgtaa ctctggttaa tgtgtgttta ttcatttaaa aatcctctgt
     4141 aactaaatag gggtcaaact cgaaatctgg ttgctcgttt tccattggct caatattccc
     4201 aacggcaata tgatgttcaa caatgccaat taatttcgcg cacatcacag caagcaaagc
     4261 gcaatattta tcgcttggta attcgtaact aagtctggtt gcctgtcgct gaagatgatt
     4321 tgtcacggtg gatgcgttaa tttcgtcaag attctttagc agataaaata gttttttgtt
     4381 gtatttcggg tggccttcac ttctacagaa cccaataaaa acatcatcaa gcttgcttgg
     4441 taactgctcg taactgtcaa agttagccag ataattttta atagtattct ttgttgtttt
     4501 gcgtactggc ataaatctgt ctcgtggcgt aatgctacga atgaccgctt caatgttctt
     4561 acgttcggat ttgctaaagg ttgggacttc gtagctggtg gggttgtttg tgtaagcgta
     4621 ggagttgtgc ttgttgcgtt ctcttgatgc ctgaagcatc gctctaaatt ctgaatactg
     4681 cattcatgtt ctgactctca taagatgtta ttattctgtt aacctatata tacaatatta
     4741 gcattttcaa agactaatgc caagtatttt taacattttc gtaacatctg tggtataatg
     4801 ggtttatggg gagagtaaaa tatgaggcgt ggctatgtat ttggtcgcat attttgcatt
     4861 gcacacagac ctcttcaatc gccctgtaac gagttaaacg acctcttcag gtcaactcat
     4921 aaggtcaggt actttttgtg tcagaatgcg attgagagcg tcttacaagg aaaactaatg
     4981 gtaaatctcc ctcctgagct ggggtcacct gatgtatgga ctggtggagg tattgggctg
     5041 attgcaggcc tgctacttcg ctacgtcctc gtcagagtgc ttgttgtctt taaactgttc
     5101 ggtcgtacat ggacgcttga ccttaaggat aacgaccaca cagacccccg ttaaactcct
     5161 tcaaaacccc tgcacgagat gacagataga agtctatggt acaggggtgt ctttagcatt
     5221 ttgtgctatc tattaaaaaa tagcttgaca gaaccggaaa gctgtatcta tcaagcattc
     5281 gcctctctga cagtaatttc taaaagttct tatttttcaa taagttgtaa ttagccatat
     5341 atagagaact gacaagtctc ttcacagaca actctgcaac cttaacagta acttctaaaa
     5401 atgcttattt ttcaacgagt tgtaatcacc caatatataa ggctgtccag taccttcaca
     5461 gataacttca agccctctta accgccccac taatcgggag aagagcgata accccagaga
     5521 gggaagctcc tttcttaacc gacctcttaa acgaccttta cagaccttgt tcaaggtaat
     5581 cacaatagtt tttcttccct cggtttcctg tgttcctcgg ctggctattg agatttgaaa
     5641 gacctctgta agcgctctct tagcgagaga aaaatcattc atagtgaccc caagcgaagc
     5701 gcccctctaa aaaaagattt tttagctatt ttgtttaaaa gcatatcaaa cgatacattt
     5761 tattgattta ggtcaaagac aaaagcattg acaaaatggt aagattgttc acatgacctt
     5821 acttatttaa gagactttga aacagcctgt ttaacaggtc actacctctg gtcttatcgt
     5881 tcccttcttt ctaggtggtt atctgttaag agtttttcag tttttattct taagataatt
     5941 gttaagatga tactctccct tcgggttata tcgtatctct tctcccgtta aatatccctt
     6001 cacagtcaga ctaaataaca cctgtgtagt tcttggtaga atctttcaag gtaacttagc
     6061 tctgagttta tgaagttgac ctatgagata gactctaccg aatctgttag tcaattgttg
     6121 cttaggtgct gctaa
//
LOCUS       DAESHI010000184         6121 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.187, whole genome shotgun sequence.
ACCESSION   DAESHI010000184 DAESHI010000000
VERSION     DAESHI010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.187"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..358
                     /locus_tag="KW011_004083"
     CDS             <1..358
                     /locus_tag="KW011_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH7662282.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPA
                     YTSQRCACCGHTAKENRLSQSKFRCQVCGYTVNADVNGARNILAAGHAVLACGEMVQS
                     GRPLKQEPTEMIQATA"
     gene            complement(398..1069)
                     /locus_tag="KW011_004084"
     CDS             complement(398..1069)
                     /locus_tag="KW011_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HBH7662283.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="KW011_004085"
     CDS             complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="KW011_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBH7662284.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="KW011_004086"
     CDS             complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="KW011_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HBH7662285.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2798..3211
                     /locus_tag="KW011_004087"
     CDS             2798..3211
                     /locus_tag="KW011_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBH7662286.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="KW011_004088"
     CDS             complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="KW011_004088"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBH7662287.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="KW011_004089"
     CDS             complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="KW011_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HBH7662288.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="KW011_004090"
     CDS             complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="KW011_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBH7662289.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(5600..>6121)
                     /gene="fliD"
                     /locus_tag="KW011_004091"
     CDS             complement(5600..>6121)
                     /gene="fliD"
                     /locus_tag="KW011_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBH7662290.1"
                     /translation="YKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAF
                     KTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKG
                     YLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTS
                     TYLSQQFTAMSNS"
BASE COUNT         1497 a         1609 c         1527 g         1488 t
ORIGIN      
        1 gccggggcgc aatgtccggg caaaatcagg tttaaaccgt tcgatactgg atcagggctg
       61 gtatgaaatg cgccgccagc ttgagtacaa gcagctctgg cgtggcggtc aggtgcttgc
      121 tgttccgcca gcgtacacaa gccagcgttg cgcgtgctgt ggtcatacag cgaaagaaaa
      181 ccgcctgtca caaagtaaat tcagatgcca ggtatgtgga tatacagtga acgccgatgt
      241 aaatggcgct cgtaacattt tagcggcggg gcacgccgtt cttgcctgtg gagagatggt
      301 gcagtcaggc cgcccgttga agcaggaacc caccgaaatg attcaggcga cagcctgaac
      361 gtagcaggga tcctcgtcct tcagggcgtg gaggatgtca acaaacaggt tgaattaact
      421 ccgcgccctg gtttttgaca ttacctaccg cgcgcgatac cgggtgccag gtgaactggt
      481 ttgctggcac acagccgttg gtagcaattt ctgatgcttc tttaccgcca atctcttgcc
      541 gcatccattc ctgtgcagct tctggcgaca gaaccagcgg gcggcggtca tgaatgtcta
      601 ccagaccctg gtctgctgca gcagtgacaa ttaaaaatcc ttcggcttcg tcaccacgct
      661 caaatggtgt gctacctatc gcggccataa aaataggttg tccatcagca cgatagataa
      721 aaaaaggctg ttttttgtcg ccttcttttt tccactcaaa ccagccatcg gcaaaacaga
      781 ttgcccgacc atgttgccag agcggtttaa acatgcgact ggttgcggca gtttctacgc
      841 gggcgttaat cagcggcggt ttatcccacc atccgggagc atatccccag aaaaccggat
      901 ccagatgaag gtgttcatca cgttcactga gcagaagaac tttggttccc ggcgcgacgt
      961 tgtatctgcc aatgggttcg ggatcgtagg gaatatcgcg ttcaatatct tccgcgagaa
     1021 gcgcaaggta atcttcacgc gtttgggatt gggcaaagcg tccacacata gaaacctcct
     1081 gagatcatac tgaaaagaat aggagagggg gcgggtaaaa tctatgtgtc agaggcaaca
     1141 atgttcccgc tgtgggccac agcgggaatg agggattact tctgaattaa ataacgaatg
     1201 gtcggcccgt cttgctgaat atccagcacc gtatagccgt gattacgcgc atccagtgga
     1261 atattattga tcgactgtgg acagtcgctc accacttcca ggatttcccc tttttttaac
     1321 tgcggcatcg cctcaagggt tgcgactgcc ggataagggc agggttcacc caccatatca
     1381 aggcggtaat caggaacgat atttttcatg cgatctcctt agcagtctgc ggtgccgcac
     1441 ggcggaagaa gcgtttttcc cagccgataa tcaacatcag cgcagtaaac aacagcaaat
     1501 atgtcaccag caggccaccc atcgggccaa aggttttcag caggttgatt ttgtcccagt
     1561 cggtggccag cgccggagcg aaatcatccc agtaatacgc cagaatcgtc gagccgatca
     1621 cattgcccag accgacccac cagtagtgca cctggccttc taccgcgcgg tacatccagc
     1681 cggtttcgca gccgcctgcc agcacgatgc caaaaccaaa cagtaaacca ccaattaccg
     1741 cgtttggtcc cgcccacatg attttgggtt caacgcctaa ctgtacgtaa ctgaagatcc
     1801 cgatggcact caccgccata ccgataatga ttgctttcgc catatgggta cgtccggtga
     1861 tccacatatc gcggaacgcc gaagtaaagc agatttgcgc acgttcaatc agtaaaccaa
     1921 agccgacgcc aaacagcatt gccagcccca gtttgggttg gttcatcgcc gtgagcagcg
     1981 cccagcccag catgccgaaa aagaccagca tcccgagacg aaaacgacgc cgcgcctgat
     2041 ccggtttttg cgtcagcggt gaggcggcag aaactttctg cattttcacg ggaatacgga
     2101 agatgggcag cagggtaaag cgcgcgccaa accatgaacc aatggcagtg gcgatggcaa
     2161 agaaccaggc atgcagcgag aactgaggaa tgccggtaaa gaacgccgcc aggttacagc
     2221 ccattgccag acgcgcgcca aaaccggcga taatgccgcc aataatggcc tgcataatgc
     2281 ggatacggct gcgcggcatg cgcagtttga cattgttggc ccacagcgct gcggcaaagc
     2341 agccgccaaa catgccgagg atcatcatcc cgtcgatgcg ggttaatggc gatccttcca
     2401 gatggataat tttaaaataa ccccactctt cagcatggac gccaaacagc tgcaggagct
     2461 gaccgcccca acgggtaaat tcacccgtga cagcccaaaa ggtgccagta atgccaaaat
     2521 agtaagtgga gagaataccc gccgcgatga ccgcagggat gggtgcccag aatttaatca
     2581 accaggcgtg tttgaattgc tgccatgaca tgaatgtagc ctctgaactc agaaggtttg
     2641 aaacaagaat cgcgaatcat acatcatgca aatttatttt cggggtaata attcacaaaa
     2701 ataatagtta gatcaaattt ctctctgaac ctggaaaggc tggcgacatc agggctttaa
     2761 tgccacaatc ctcttttctt ctcatgcagg acgaatgatg aaaaaactcg caattgcagg
     2821 tgcgctcatg ttgctggcgg gctgtgccga agtcgaaaac tataacaatg tcgtgaaaac
     2881 ccccgcgccg gactggctgg cgggctactg gcagaccaaa gggccgcaac gtgcgctggt
     2941 cagtccggaa gcgataggca gcctgattgt cactaaagag ggcgataccc tggattgccg
     3001 tcagtggcag cgggtgattg ccgtgccggg caagttgaca ttgatgtctg atgatctcac
     3061 taacgttacc gtaaaacgtg agctgtacga ggttgagcgt gacggtaaca ccattgagta
     3121 cgatggtatg acgatggagc gtgtggatcg cccgacggca gagtgtgccg ccgcgctgga
     3181 taaagcgccg ttaccgacgc cactgccgta atgaaatctc cccggcttgc cgccggggat
     3241 gaatttaaat cacctcttcg ataacccaca cgctgacgct gccgccgttg caaaagaagg
     3301 tcgcttcgcc gttttcgtcg gtcactacgc tctcttgccg attccccaga aaatcacgcc
     3361 aggttttatt gccgtaattc tctcccagat gaatggtttt ttcgccatca tccccgttcg
     3421 acatgaccac cacgcagccg ggatattcgt cagtgccact gcggctaaag gcaatgcagt
     3481 tcggatggtc gaaaaataac gtctgtacac cgtgggcgaa acgctgacgg gcgagaatta
     3541 actcatcaag ctgttcgatt attggcatat ctatcggata ggtttgcccg tcaccaccga
     3601 catcttcgta atgcgcaccg tagaggtccg gatagaacac cgaaggaacg ccattttccc
     3661 gcaacaaaat tagagcatac gccagcggtt taaaccacgg ttcgaccggc gcttcgaggg
     3721 cttgcaacgg ttgggtgtcg tgattggcta cgagggttac ggcgtggaaa ggatcggctt
     3781 ccactaatgt acccgtgaaa atctgcgtca tgtcgtagtc gcgtcccatg cgcgatgctt
     3841 catggaattt catctgcagc ggcgcatcga acagcatggt tttgccttcc acctgatcaa
     3901 tatacgtttg cagtttatcg acttcatgcg accagtactc tgccacaata aacagcggct
     3961 ttggcgcaac ttcctgtacg tgttcgatcc actctttata aaaccacgcc ggaatatgtt
     4021 taaccgcatc aagacgaaaa ccgtcgcatt gtgtttgttc catcacccag cgcgcccagt
     4081 atttaatctc ttccgtcacg gcatgattgc ggaaatcgat attctcaccc atcagataat
     4141 cgaaattacc gagttcatca tcaacctgat cgttccagcc ttcgccggtg tagtcgttaa
     4201 caattttaaa tatgccatct tcatcaggat tttcgatatg gtcgataccg ctaaaacatt
     4261 tgaagtccca gataaactgc gagtattgcc cggcacgggc ggggaaggtg taacgcgtcc
     4321 agccttcgca ctcaatgatt tcttcatcaa tttgcgtacg gtcatcagca tttacacgct
     4381 gcacgcgaat agcttctttt tcatccgccc ccattttgtg gttgactacc acgtccagta
     4441 gcaccgcaat gtcattacgt ttcagagcgt caatggcggc cagcagttgt gctttatcgc
     4501 catatttagt agggatgctg cctttctgat caaactcacc taaatcaaat aaatcatagg
     4561 agtcgtagcc gaccgaatac ccgcccgatg cgcctttata ggcgggcggc aaccagacca
     4621 tattgatacc aatatcatta aaaccgtcgg cgcgctcggc cagttcaggc cagagcttac
     4681 cgccttccgg gtaataccag tgaaaacatt gtaacagcgt gggattacgc attcttcgac
     4741 tccattcaag gggaacatta gaagcgtagc cgtaatcgga ttattcgcga gccatcggct
     4801 cgttaagatt caaaagaggt tatcctgcgg agccagcaca aagccgccct gatctccata
     4861 ggcacttaac actgattttt gcaccgatga ctgaccgacc agtttcgcca gttcatccat
     4921 acgaatctgt aataactgct ttaccttgct ttcgttgtcg agaatcaggc gcagcatcgg
     4981 gcggagctgc tcctgcatcg tggtggacgg ggcaacctct tcagtcagat gtgcaatctc
     5041 ctgcaccgca ttcacatacg ccatttcgct ggcgatgagt tcgtcccatt gttcttccgt
     5101 tgccaggcgt aacatgagct ggcttttatc gacgagttgt tgccaggcga aatacaaatg
     5161 cggtgcatgg ttcatcagac tgggtcctga atcaaagaag gggagagtaa agactctttc
     5221 caggcatcgg caatattgcg cattaatgct tccacttctt cgactgcgga gacatcgttg
     5281 cgtaaattgg cttgcagcaa gcgcctgacc atatagctat aaagagcaat caagttttgg
     5341 gttagttcgt ctttgctctc ttcatcaaga ctcacccgca gcccgttctc aatgatgttg
     5401 atcgcttttg acaaagagac gcctttgcct tgctgattgt tgtcctgcat aaacaggcgc
     5461 gctctaacca gtgcgctcag cactccatca aatagcatgg tgaccagctg ttgctggctg
     5521 gcgctcatta cggcgctttc gacgccaatt tgtgcatagg cttttgagcc gctcgcggta
     5581 tacatgctga cctccgtgat caggaattac tcattgcagt aaattgttgg ctgagatagg
     5641 tactggtgtt attcagtttg ctcatcatgg tatctaattg cgtaaactgt gcagtgtaac
     5701 gcgccacggt gtcatcaatg ctggcactga cggatagata ctgtttggtc agttttttca
     5761 gtgtggcgtt aatactgtcc tgtgcgctat caataatgcc atcatcggca aggtaacctt
     5821 ttacctcggt ggcaattttg gtggtaattc ctgtttcttt accatcgcct accagcaatt
     5881 cacgtacgga tgctgtattt tcattcagga cttttttgag tttatcatca tcaattttca
     5941 gcttacccgt cgtgccgtcc tgcttaatcc cgatctcgtt taatgttttg aaggcactgt
     6001 cgctggcacc gtttgcaaat tgtgcgcgga tgccgctttg aattgtacgt acaacgctgt
     6061 ctccgagaag tgcaccatta tttttatctt gtgcttcagc acctgggtct acttctttat
     6121 a
//
LOCUS       DAESHI010000185         5934 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.188, whole genome shotgun sequence.
ACCESSION   DAESHI010000185 DAESHI010000000
VERSION     DAESHI010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.188"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            295..371
                     /locus_tag="KW011_004092"
     tRNA            295..371
                     /locus_tag="KW011_004092"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:329..331,aa:Arg,seq:tct)
     gene            complement(416..1177)
                     /gene="envY"
                     /locus_tag="KW011_004093"
     CDS             complement(416..1177)
                     /gene="envY"
                     /locus_tag="KW011_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HBH7662291.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1360..2250)
                     /locus_tag="KW011_004094"
     CDS             complement(1360..2250)
                     /locus_tag="KW011_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HBH7662292.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYIRAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2251..5223)
                     /gene="nfrA"
                     /locus_tag="KW011_004095"
     CDS             complement(2251..5223)
                     /gene="nfrA"
                     /locus_tag="KW011_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HBH7662293.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5210..>5934)
                     /gene="nfrB"
                     /locus_tag="KW011_004096"
     CDS             complement(5210..>5934)
                     /gene="nfrB"
                     /locus_tag="KW011_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HBH7662294.1"
                     /translation="DTALRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESID
                     AWQIPSSLIAEMPASVALHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRY
                     FIVLRGQIVTGLRHWYARRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAE
                     ILTTLGHINRSAINVLLLRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQS
                     LLLKAGLNTEQVAQLESENEGE"
BASE COUNT         1348 a         1562 c         1549 g         1475 t
ORIGIN      
        1 acattctgat gataggatga gtatccataa tgatcaccga cgttggtttc atagttacca
       61 gtctcatagg agcggacaat tttccgttag ggcggtaaat tgtactttga tacatgaaaa
      121 tacgggtttt cttgattcaa acgcgcagcg gtgtgcattt gtttgccgct atagcgtaat
      181 aaatcagaaa atcagacgcg gtcgttcact tgttcagcaa ccagatcaaa agccattgac
      241 tcagcaaggg ttgaccgtat aattcacgcg attacaccgc attgcggtat caacgcgccc
      301 ttagctcagt tggatagagc aacgaccttc taagtcgtgg gccgcaggtt cgaatcctgc
      361 agggcgcgcc atttcttctt catttaaccc cgtcttatcc gttccgcttt gctcttcacc
      421 acatcacttt ttgtcgctgt ttggcgagat aattcaacgg tgtcaggccg taaaacgcct
      481 taaaaacaga gataaagtac gacgtgctgc tatagccaca taattgcgcc acctgagtga
      541 tatttttgtt atccatcaat aacatctgta cggcgtaacg catacgacac tctgtgacaa
      601 tctggctata gctgatattt tcgtttttta atttcttttt gagcaggctg gggcttaaac
      661 atagcgaact ggcgacaatt cgcagattcc agtaatgctg aatatcgctt tgaataatgc
      721 ggcagacgct gtcgcggacg ctgctgcgta agatatacat cagtagggca ataaaccgcg
      781 attgctcaag aaagttagac aatacggtaa aaagcaatgc gcgcgtcaac tccgcttcgc
      841 agggtttgct gggttgctgc ttgctgtggt tggcggctag ccggaacact tccggtgtac
      901 ggctatgaca agcgataacc ggggttgcca gccgcgacca gggctttaca caggttaagt
      961 tcttatttaa aaacaacaga taatcgttga tgatgttacg gttgatgtgg gtgattaata
     1021 cgtgatttaa cgttgagagt tcaatgacgt tattgttgca cgcgaaggcg gccaggtagt
     1081 ttttcggaag ggtaaacgta gcatggttat tgacgcttac ctctacctct ttttcggtca
     1141 ggatcaccac gcaaggttca ctgctgctca attgcatttc gcactcctca gatatcagaa
     1201 actccgctca aaggatctat gcttcctgca tgagtgatcg gcccgttcgc cgataacgat
     1261 cttctttctt tagcacgctt tttagcaatt aatcttgatg gaattctgac gagagcgaaa
     1321 gaggtaagcc aggtcgtacc cgacttacct ggaggagatt taatactcga gaatgccgtg
     1381 cgcgacgggc aaatagcgca gagagaaata gagagtgtct ttaccgcaag aggaacgttt
     1441 cgctaacagc gaggcgatct ctgcatccgg tttcggtttc gctgtaaagg ttttgccttt
     1501 gccggcgaca aacagttcat aaacaatgcc gcttgctggg gcggagcttt ggcaatcggc
     1561 gctggcctgt agataacgtt cacgctgttc agcggtaagt ttatccacgc cgctgccatc
     1621 ctgtacccag acattaactc cggttgcttt aacttgttcc agcagttggc gatagccatc
     1681 tggcgacatg tttccggcga aaaaactact gatataaacc ggttttgctg aaacatcgct
     1741 aatcagccgc tgcgcgttgt ttaaccatgt tagcaaaggc tgacgggcag cttcgctgcg
     1801 ccagttcagg tcgtcaattt ccgccctgat gtaccagcca tccggcgtta tgccaggcgc
     1861 ggcgctccat aatctggctt gctggagatc ggcagccagc aggcgattaa gatagctttc
     1921 cagcgctgcg gacgactgtt tctggtgcat aaaaaattcc ggatcggcgt tcagcccgac
     1981 aatcagtttc aggccagcct gttgcgcagc tgcggcacgc ttaaacaata acgtgcgctg
     2041 ttctggctgg gtaaatgcat cgccgtaacg ggtccattgc aaaaccaggg tatcgaagcc
     2101 ttgcaaacgt aactgactca tcagcccctg ccactgggta tcggtaacct gactatctcg
     2161 gttttgtggt tgccagataa tacctttcat cgcaaaggaa aaagggctga ccaaaagcag
     2221 tgtcagcaat acgaaaatga acttacgcat ttaccagtgc actccaatgg tgagaaacgc
     2281 gttgttgcgc tctccgttac gttgattaat cgccttaaag gtatgttgat actcgacgcc
     2341 gagactgact ttgtgcggcc aggcgtcgta gtgcgtctcg ccggtccaga tattccagcg
     2401 gaccccgact ccgccaagct gcgcgccctg agtgccttta tcacgatagc cgttgtcctg
     2461 aacgtgagcg taaggctcaa tagtctgtcc gttagctacc ttctgatgcc agctgacgcg
     2521 ataatctgcc gtccacgcct gaatatcctg gcggatatat tgcgccgcat cgaggtacag
     2581 gttttgggca aaccagcctg aaccgttcgg gtgccattcg tcgctgtatt tgccgccatt
     2641 aaagaatgag gcgctggcgc gcagcatggt atcggatgcg ccattttgcc cgttcagcgg
     2701 caactgctgt tcgacggcga ggaaaaagat ctgatcgcgc agcggcttcc agcgcagacc
     2761 ggtgccggac atcggatttt tcaccggcat catcaccccg ttttctccgg tatcggcaaa
     2821 gacgcggcta taaactgaga gcaggtcgcc ttccagcagc atattgcgtc cgatgcggta
     2881 ctcggcttcc agttgcccgt agctacgata gcttttccct ggcgctgcgc cgccaacatt
     2941 attgttagcg gtactcattg ccccggaacg caagccgata gaagaatcga aactgaacgt
     3001 ccagcggcga ccgacctcct catgcaaacg gcggaaattg aagcgttgtt gattttgctc
     3061 tggggtcagt ggggttatca gcgcctgatt atcaatgtca tcaatcacca gccgggcgta
     3121 gtgctgcgtc gcaggcatgt catccagacg ctggttcacg taggccagtt gtcggatcag
     3181 tgccggatcg tccggaagcc ttttatgcgc ctgttcgagc atttcccgcg actgtgcgat
     3241 atcaccgcta tcccacaagg cgtaaccaag cgctgcctgg atgttgctat tattcggttc
     3301 cagttccagc gcggcgcgca aatcactcac cgcggccggg acattatgac gttggcgata
     3361 aattgtcgcc cgcgcaacgt aagcgttggc agaaggcgca atattgattg agcgcgtgag
     3421 atcgttcagt gcgagttccg gctgaccagg aatgtaacgt tgtgcatgca gccaccagta
     3481 gagggcattg cttcccagtc cacgttgttc tgcctgttgt agccagcgat cgcgagccgc
     3541 accatttcct gccgcctggg cggtattggc agcagcaagc agatcctcat tgctcatgtc
     3601 gtgaagactg attttctgcc aggccgccag tgcggtggcg tagtcctcaa cctgatacgc
     3661 ctgataggct accgcacgat gttgccaggc gctcggttgt cgttgttcgg cctgaagcca
     3721 tgcatacaac gccacaccgg gtagcgtgtc ccgataacac tttgccagac ggttccaggc
     3781 ggcagcatcg taggaaggcg acatatcgcc cagcaagcga actattgccg ggcaattatc
     3841 tgcaataccc ggcaactgac tttgccactg acgttgctcc gccagcggta agggtttcga
     3901 taaaatcgcc accttcgccg gcgttgccag gtaaggatga ctttccagca gagacgccag
     3961 tcgcgccatt aaagtctggc tgacacgcgc atcgccctgg aaaggatagc gttgcagcaa
     4021 taaatcggca gcttcgcgtg actgctcgtt ctgcatcagt tgccaggtca gttgatccag
     4081 gcgggtaaga tttgccggtt cttgctgata cagcaatcgt gccagacgca gagattcagc
     4141 cttgttacgg gtcgccacgc tgacagcata acgctcctca agcatttcat tggcggggag
     4201 ggtggcgagc agtttttgcg ctgcgtcgta ctgaccttct tttaacagca ccggtagcgt
     4261 cgcgccaaca acatactggc ggttatcggc aaactgcacc gtataattcg ccaatgcctg
     4321 aacggggttg gcgctgtatt tagataacag atagagccaa cttttctctt gtgcgtccgt
     4381 ggtaaacagc ggcttatttt cattgagata atgctggagg cgtgcttttt cgccacgata
     4441 agccagcgcg gtcgcgtaag taatatatga ctgaggatcg gtgaagatcc cctgtgattg
     4501 cagtgctagg atccgatcgt ccagctgccc ggcaagaagc acgtcaaacc actgacggcg
     4561 ttctgccgcg cttaatgtgt tctgctggcg tgcttcattg tatagcgtat ctgcctggga
     4621 ccattgtttc aggtagattg cccgttgcag cagatcggtt cgcagcgttt ttccttccgg
     4681 cgatgcagca aacgtcgcat cgttcagttg cgctctggcg acaggtaact gtgcaagccg
     4741 cagggcattc tgcccgactt cactgcgaca acgcagggtc ggcgcagcat cgcacgcttt
     4801 ttgctgggca agcagttctt caacagttgt aacgcttttc acttcaaccg gaatagccgc
     4861 cagactgcgc tcaagtcggg catctcctgg gtgacgtttc agttgatcct caagcaacag
     4921 ccgcgcccgg tcatcatgac caaaatggcg ataggcttcc gcaaggtata aagtcagcgg
     4981 aatattatcc ggcacctgct ggtgtatata ttcaaattcg cggatggcgg ttgcttcgtc
     5041 gttatttttc tgtgccttca gcgccttatc gagacgggga taaataacaa aatggcgata
     5101 atcgctcagc cccagctctt ctgcgctggt gccgatattg tctgcgagtg cgctggtact
     5161 caataaagac gtcagcagta aaccagacca tccgatgacg cgattaaggt tattctcctt
     5221 cattttcgga ctccagttgc gcaacctgtt ctgtgtttaa acctgctttg agtaatagtg
     5281 attgcatcga aacttgtaat tcgcgttgaa ttgtcaggac gcgatccaac gtttcctggc
     5341 tgataacgcc ttcggtgacc aaaaacttgc cgagcggcag agaactgcgt tcatggcgca
     5401 ataacaacac gttaattgct gaacgattaa tatgaccgag cgtggtcagt atttcggcga
     5461 acaggaactg atgcggcaca tattgccgcc agatttcacc ggcctgctgt tccgtgagcc
     5521 actgatgctg aaccgcattg tacaacattg cccgcggatc gtgaccgcgt cggcgtgcat
     5581 accagtgacg taaccccgtg acaatttgtc cccgcagaac aatgaagtaa cgcactttgc
     5641 gtccgacttt acgcgtcagg gccgccagcg aaaccgggtc aataccatct tcactgccga
     5701 caattaactc atcattttcc agacgcagcg gcagtaccgc ataatgcagc gccacggagg
     5761 ccggcatttc ggcaatcagc gaggaaggga tctgccaggc atcgatggat tcccacgcca
     5821 cgccgttttg ctctgccagc gcctgtgcca gctgctcggc gctaatcagc ccctgcatca
     5881 gcattgaacc gcccaagcgt agaccttcga cgcgattacg cagtgctgta tcga
//
LOCUS       DAESHI010000186         5865 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.189, whole genome shotgun sequence.
ACCESSION   DAESHI010000186 DAESHI010000000
VERSION     DAESHI010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.189"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..560)
                     /locus_tag="KW011_004097"
     CDS             complement(<1..560)
                     /locus_tag="KW011_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662295.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIR"
     gene            complement(704..1462)
                     /gene="aroD"
                     /locus_tag="KW011_004098"
     CDS             complement(704..1462)
                     /gene="aroD"
                     /locus_tag="KW011_004098"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBH7662296.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(1493..2359)
                     /gene="ydiB"
                     /locus_tag="KW011_004099"
     CDS             complement(1493..2359)
                     /gene="ydiB"
                     /locus_tag="KW011_004099"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HBH7662297.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(2371..3636)
                     /locus_tag="KW011_004100"
     CDS             complement(2371..3636)
                     /locus_tag="KW011_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH7662298.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3863..5077)
                     /locus_tag="KW011_004101"
     CDS             complement(3863..5077)
                     /locus_tag="KW011_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH7662299.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(5177..5533)
                     /locus_tag="KW011_004102"
     CDS             complement(5177..5533)
                     /locus_tag="KW011_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HBH7662300.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(5669..5776)
                     /gene="rprA"
                     /locus_tag="KW011_004103"
     ncRNA           complement(5669..5776)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="KW011_004103"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT         1690 a         1339 c         1330 g         1506 t
ORIGIN      
        1 cgaatgaagg cgatatctgg cgcaatcgct ttgtaataga gatattcttt gttatcaaac
       61 tcgaccagtt taatcaggtc ttctttcgtg acttcattca gtttgccgcc ttgctggcgt
      121 ggatcgacaa atgtcccgat gccaatatca ctaataatac caggctggtg ggctgcggcg
      181 gcgcgtaagg tttgtgtaag tacaccttgt ggatagttat aagcaataat tttattttgt
      241 tctgcgagtt cagaaatacg cggcgattgt ccccagtgac cacataatgc ccatttcacc
      301 agaccttctt gcgccagagg actaatacca cggtcggcgc gatcgccaag ccctgtcgga
      361 ctaataatcg ataaattacg tggcgtttga gtctgtttat atttatcagc aagagctgta
      421 attaacgtgg tggcttccag aataccgccg ccagcgccta acacacaaag tgttgcttcg
      481 tcgggaatat aattcaccgc ttcctgtgcc gacaggaccg gcactcgtcc attaatacga
      541 ggtggtttta caggtttcat tgttaattcc tgattcagat gtgattttcc ctacgccaga
      601 tggagaatta gcgcacagag actcacgaaa tatttttatt aaatacggtt aaatatattt
      661 tttaagttcg gcggggaggg tgttcccgcc gaaatattat tgcttatgcc tggtgtaaaa
      721 tagttaatac cgtgcgtaaa tcatttaccg agatttgccc tggtgcagag gcttttttta
      781 ccgcaccaaa agttgccgcc gagccaaata cttcaccagc cagacgagaa attacgccag
      841 tttttgccat cgacatcgtg atgattggac gatcggcata ctgctcctgc atctccaggg
      901 tcgcggtaag caacgtcagc acatcgctgg tactttgcgg catcagcgca atcttaggaa
      961 tatcggcgtc gaaggactgc attttgcgca gacgggcaat gatttcttcg gcttccggcg
     1021 ttttatggaa gtcatggttg gacatgacaa ctttcacatc atgcgcgtgg gcgtaggcga
     1081 cggtttcttt aacctgatca tcgccggtaa ataactccag atcgatcata tcaaccaggc
     1141 cgctgtcgat ggctgcacga ttgagagcaa tataagcctc ggtggaaatc gcctgctcgc
     1201 cgccttcttt ggcactgcgg aaggtaaaca gcagcggttt ttctggcatg gtttcacgga
     1261 gaatttttgc cgccgccatg acagactcca cattggagag gtcggcaaag tggtccacac
     1321 gccattccag aatatcaaag tccgcttcac gataggcgag agcttcggat ttcacgcggg
     1381 cgatatcttt cgccatcagc gagacgatga ttttaggtgc gcccgcacca atgacgagat
     1441 cttttacagt tacggttttc attttttacc ctttctgcac gcggtcagcc tgtcaggcac
     1501 cgaaccccat gacctgttta acatattcca gagggaaatc tttgccagtc cataacgtga
     1561 actgttcagc cccttgccac aacaacatgc cgtatccatc aatcgttttg caacctgctt
     1621 gttgtgcctg ctgcaataac ttcgtcatat gcgggttata cacgcattca gtgaccagca
     1681 gtcccggatg taacagactg atatcattaa ccaatgattc attctcaagg gggttcatac
     1741 ctacttttgt gccattggtt aaaatgtcgg cggaagccag gacttcagca aaggcttgct
     1801 gatcggcgag atcggtaacc gtgacgacac aatcggtgtt ttcgttaacc cgctgcgcga
     1861 aggcgagggc tttatcgaag aactcatccc gacggttaaa gagtttaatt tcttttaaac
     1921 cttcaattgc cccctgcgcg ccaatcgctg ttgaggcacc tccggcccct aacagcacca
     1981 tcgttttgcc tttgatatca aaaccgctct ctttaatggc gcgaatatgg cccgtgccgt
     2041 cggtgttata gccacgcaga tagccatcat cattaacgat ggtgttgatg gcacccacca
     2101 gtttggctgc tggcgttaat tcatcaacat attcacacgc cagttgtttg ttcggcatcg
     2161 acacaccagt tccgcgcatt ttgagggctt ttaatccttc aattgctccg ggaaagctat
     2221 cgttatccac ttcgaaggcc atataggtaa atggcaatcc cgctttttct aaggctttat
     2281 tctgcatttc aggcgataaa ctgtggcgga taggataagc catcaaccca atcaattcgt
     2341 attttgcggt aacatccata attaactcct ttaacctcta tgcttaattg tgtttaaccg
     2401 ggtgctaaat ttgcgttcgc caaaccgcac atcattctca ggaataataa aaacgcggta
     2461 atagcggcta aaaacaataa ttgcggtaat cagggccagc agcgcgaaag taaaatcgag
     2521 aacaatgata tattgcaggc cgatgttcga cagataaccg gtaatcagcg gaataacaaa
     2581 gttagccagt ccacccatca tcatataaat actggtgact ttggctttgc ttttggggaa
     2641 aaactctgac atgaccgaaa caccgagctg taaaatgccg ccagctgctg agaaaccgat
     2701 aacaaaggcg ccggcattac acaccagtgg agaagggtac aggtagataa tggcagctgt
     2761 tattgttgcc agcgcagagt taaatacatt agcccagatg ggccggacca tttttttcag
     2821 tagtgcggca aaaataaaga cacagaccaa cgagcccata ctgtaataag agatggtttt
     2881 taatgcctca gcttctgaca taccagcaaa agccatcgca tatttgggca tccacaccac
     2941 aatcacataa aaggtcgaga atgcggctac accgaacagt accgatgaaa caccttccag
     3001 ccagactaac ggtttgctgt tcatttgcgg taattcatta gctacgctgg cgtccaccaa
     3061 ctggctgggg aatttgcttt tcaacagcat cagcgtgatc agtacaaaca gaatacccgg
     3121 aataatcagc ccatagccgt accagatatt attgagcaac atatagctca ccagcattgg
     3181 gtagaacatt tgcccaaatg acaccatcgc tttaaccagt atgaccgccg aaccagaggc
     3241 tttcggaaag cattccatga gcgcggggta gccacccgta tccagcgctg agttagcgat
     3301 acctacgcac actgccagac cgtaggcgag agttaaattc gggcaagcgg gaataccaaa
     3361 gaagaatagc agatacatta ttactgccat taatatcacc gcccgacgac caaacttatc
     3421 ggagatcaca ccgaagaata aaatactgat caatcgcccc aaaccgatac cggaaattaa
     3481 ataggcaatg cccgcgttgt cagtggaaaa cttttctgcc agagatgtca tattttgggc
     3541 aagcgtaata acactaatgc cgtgcaggaa atagctgaag taaatacaaa gaacagccag
     3601 gataaatggc gtgctgaaag ccttattttg agacattttc acagctcctt gctgaaagat
     3661 aatttcgtta ccgtacgtat tgttctccga cggtttacat aattactata ctgaagatga
     3721 ttttccctgg gtacatggca taaagtttat gtttccagga atattgcatg tcatagtttg
     3781 tttttgatcg cgattttcct tagttagcgc agaaagagtt atttatccgt aagatcttgc
     3841 atgacctgaa aagaatattg cattacccac ccggagcgac attttccttt agcgagtgat
     3901 gccgcgtttt tttgcggctg cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag
     3961 cgatggcggt atcgaaccac ataatatcgg caatacttct ttgggacaga tgagccgtaa
     4021 tcaacggaat agtaaaggtc gcaatactgc ccgcactgta atagatccct gtagctttac
     4081 ctttagcgta agggaaacgt tcagccatta acgtcaggcc aatttgcaca acacctccag
     4141 cagaggtaaa accaatgaca aaagcaaata ttatcaccac ataaaatgtg ggatgcaggc
     4201 agacggtaaa cagagcaata aatgagataa aggtgtacag catcagtaat gttgttgggc
     4261 gaacggtatt acgaatgagt ggagcggtaa taaatacaca aagcagcgaa cccacggtgt
     4321 agatactgag tagtttgatc gacatagtgt atgacatgcc tgcaacaaat tgtccgtact
     4381 gtgccagcca ctggctaacc agataaaacg ttgccattga gatatagcca tataaggtat
     4441 aactggctaa atcgataatt gaacagcgat gttccgtaga gctggtggtt ttctttatga
     4501 caggtaagcg acgacccgga tggggtggga acgtacaacg gtataaaaac agagcgttaa
     4561 taaacataat gcctgcagca atcataaagg accaaccgaa ccacagttca gcccacacta
     4621 acaggctaat gattagcggt aataaaaatt gtccgctgga aacaaatgct ttaattaaaa
     4681 tattggctgt cccaggtgag cgtggaaaag cttccatcaa actgggataa gtgcctgcat
     4741 cgagaaaact gtttgccatt cccgccagaa agccaaaaac ataagcgata atgatgttat
     4801 tggtctgcag gatgccaaaa aagaaggcca tatagcagca catcccgagc atgataaaag
     4861 ggcggcgacc aaagcgatcg gataataatc ctgcaaaaag caagacactt aatcgaccaa
     4921 tgcccagcga tgagataact atcgagacac ccgcggcatt agtctgccaa agtgtctcca
     4981 gcgaggccat attcaggctc atcaaaatga cgcccatacc atgcaccagg taattaaaat
     5041 acaacccaag tgcggtaggg taatagggat ttttcatagg ttctacttag caaaggtgct
     5101 ttaaaaagtg tagcgatgtt gtgtcagtat ccattcatta ttgttgtaac aacgacaatt
     5161 cctttctgtg gaaaaattaa caaagccgct ccagatcgtg tgcatataat tcagcggcta
     5221 ccgattgata gatcttccag tcaataaaat tgccatcagg gtagacttgt tgaaacgcgg
     5281 ttaagtcggg gaaaaaacgc atggtgttgt tgccgatgcg gttatttatt ttctctatga
     5341 tggcatgaag atgttttttt cgttgctgac gcatagccaa cagttgctcg actacacaat
     5401 caggaatggc gcatttgcca ttttcccact gacgccagct ttcactgtca ccggtctggg
     5461 caatccaggt cgcgcattca tcaatagtca tggcaaaaat atggcggagt gcctggagtt
     5521 cataagcgtt catcgtgtaa tggctgcatg aaataaacaa taaaatataa ataacattat
     5581 cagcctgctg acggcttgaa gagagtcaca gtatcttgtg caacattatt ggaaagagtg
     5641 aggggcgagg tagcgaagcg gaaaaatgtt aaaaaaaagc ccatcgtggg agatgggcaa
     5701 agactacaca cagcaattcg ttgtttcact caggggattt ccatgcttat aaatcaatat
     5761 gttgatttat aaccgtgagc taatagtagg catacggact ttttgcgtcg atcagattcg
     5821 tctcattagt ttaaaaactg taaagaattt gcgacaggta ctacc
//
LOCUS       DAESHI010000187         5805 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.190, whole genome shotgun sequence.
ACCESSION   DAESHI010000187 DAESHI010000000
VERSION     DAESHI010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.190"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            353..664
                     /locus_tag="KW011_004104"
     CDS             353..664
                     /locus_tag="KW011_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH7662301.1"
                     /translation="MSIEISKKLKAIRESEGLSQAKFADSIGIAVGTVKQYETGIRGV
                     GTEVLLKITMHPEFKKYTTWLMSNETNEAAGQISPSLSPDGPENTSSSQKSRKTGTQP
                     G"
     gene            753..1691
                     /locus_tag="KW011_004105"
     CDS             753..1691
                     /locus_tag="KW011_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH7662302.1"
                     /translation="MRPEGRKGKRIRKKFKTKSDAVLYERWVLAQQHNNEWKGNSIDR
                     RPLSVLIDLWWKYHGQLMKSGHNTRLKLLRLSEAMDDPCVHKLNTTMLTELRVSRIEQ
                     GIQPSTINREIGALSAMFTALISSGHFLNDNPVQGLKGMKVNEREMGYLSKSECVQLL
                     DALAENPDERLAVEILLSTGARWGEVAALEQRRVLHCRITFSKTKNSKNRTVPISESL
                     FEKIKKRGGKLVFPTLDYSLVRDVIKTVAPDVPDGQAVHALRHTFASHFMMNGGNILT
                     LQKILGHAKIQTTMIYAHLAPDYLQDAVRFNPLGGM"
     gene            1694..2194
                     /locus_tag="KW011_004106"
     CDS             1694..2194
                     /locus_tag="KW011_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662303.1"
                     /translation="MEIKKPTKKELYDYLLSKYIEDKCKEEADEINKKSMSRVKKHKE
                     RLMEITPEIFFRFLSEKGVSSVCPSCGSSRLSVPESMDLCWDKNKKPENFNNLPLEEQ
                     SELIKENIKHYVSYAFLGDVKSIPDMRKTYYTLHCLNCGYLSLYRTSAVLKWLEKDKA
                     QDGDNG"
     gene            2295..2732
                     /locus_tag="KW011_004107"
     CDS             2295..2732
                     /locus_tag="KW011_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1640 domain-containing protein"
                     /protein_id="HBH7662304.1"
                     /translation="MSDKLERRIERLEGDLSLTRNDLATLAERTTNLSTKADVGEVKG
                     ELKADIAHLKGDLECDIANLKGELKSDTANLKEQLKSDINSLKGELTEAMDKRFDKIM
                     DEMNRRFDKVDDNTKWRWSGIIVPVCTTIFTAAVAVFVAKFVG"
     gene            2925..3905
                     /gene="btuC"
                     /locus_tag="KW011_004108"
     CDS             2925..3905
                     /gene="btuC"
                     /locus_tag="KW011_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HBH7662305.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            3968..4519
                     /gene="btuE"
                     /locus_tag="KW011_004109"
     CDS             3968..4519
                     /gene="btuE"
                     /locus_tag="KW011_004109"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HBH7662306.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            4519..5268
                     /gene="btuD"
                     /locus_tag="KW011_004110"
     CDS             4519..5268
                     /gene="btuD"
                     /locus_tag="KW011_004110"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HBH7662307.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            5346..>5805
                     /locus_tag="KW011_004111"
     CDS             5346..>5805
                     /locus_tag="KW011_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBH7662308.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
BASE COUNT         1465 a         1261 c         1564 g         1515 t
ORIGIN      
        1 ttctctgcgt ttctgatggt tttcaggaaa attgcgaaga gatttacttt gcgttttgtg
       61 tttcttcctt cttgagtaac gggaattacc gatctatcag cctgccttct tacagcgaga
      121 atgctttgat ttgtgcgttt cgcataatcc tctaggcttt cttcaagtac cggtagccca
      181 tgttcattac ggtatgggta gaacgccgcc aaacgctcaa aatccgcttg ttcgtatgtg
      241 ttaaggactt ttgccatggt gtgataacct attcaatctg gtgctatttg tggctctttg
      301 tagcgtcaag tggtactcaa ctgataacca atatagtatt caggtgcaca ccatgtcaat
      361 agagatatca aagaagctaa aagcaattcg agaatctgag gggcttagtc aggcaaagtt
      421 cgcggattca ataggtattg cggttggtac ggttaagcaa tacgagactg gtattcgagg
      481 tgtgggaacg gaggttttac tgaaaatcac aatgcacccg gaatttaaaa aatacactac
      541 gtggttgatg agtaacgaaa caaatgaggc tgctgggcag atcagtcctt ctctctcccc
      601 tgatgggcca gaaaacacat cgtcttctca aaaatcccgc aagactggca cacagcccgg
      661 ctaatcatgg aacgctgggg gcatggtggt cttgtaacgc tggggcttca cgaatgagca
      721 taaaatcaat tccgggaggg tatcttcttg acatgcgtcc tgaggggcgt aaaggcaaac
      781 gcattcgcaa aaaatttaaa acgaaatcgg atgcagtttt atatgagcgg tgggtgctgg
      841 cgcaacagca taacaatgag tggaaaggaa actccattga tcgccgtccg ctgtcagtgc
      901 ttattgactt gtggtggaaa taccacggcc agctaatgaa gtcagggcat aacacgcgcc
      961 ttaaattgct gcgcttgagt gaggcaatgg atgacccgtg cgtgcataaa cttaatacaa
     1021 cgatgctcac cgagctacgt gtgtccagga tagagcaggg gatacagccc agcaccataa
     1081 atcgagagat tggggcgtta agcgcgatgt ttaccgcact catctcatcc ggccattttc
     1141 ttaacgataa ccccgttcaa ggccttaaag gaatgaaggt taacgagcgt gaaatgggat
     1201 atctgagtaa gtctgaatgt gttcagttgc tggatgcact ggctgaaaat cccgatgaac
     1261 ggctggccgt cgaaatcctt ctgtcgaccg gggcgcgatg gggcgaggta gcggcactgg
     1321 agcagcgccg tgttcttcat tgtcgaatca ctttttcaaa aacgaagaac agcaaaaacc
     1381 gtaccgttcc tatttctgaa agcctgtttg aaaagatcaa aaaacggggc gggaaactgg
     1441 tgtttccgac gctggattat tcattggttc gcgatgtcat caaaacggtc gcacctgatg
     1501 ttcctgacgg ccaggctgtt catgcgctgc gccacacctt cgccagtcat ttcatgatga
     1561 acggcggcaa tattctgacg ctccagaaaa ttctggggca cgcaaagatt cagacaacga
     1621 tgatttatgc ccatcttgcg ccggattact tgcaggatgc ggtgaggttt aatcccttgg
     1681 gaggaatgta actatggaaa taaaaaaacc tacaaaaaaa gagttatatg actatttact
     1741 atcgaaatat atagaagata aatgcaaaga agaagctgat gaaatcaata agaagtcgat
     1801 gagtcgtgtc aaaaaacata aagagcggct gatggagatt acgccagaaa tcttcttccg
     1861 tttcttgtct gagaaggggg tctccagtgt ctgcccttca tgtggatcgt cgcgattatc
     1921 tgttccagaa agtatggatc tttgctggga taaaaataag aaaccagaaa attttaacaa
     1981 tctacccctg gaggaacaga gtgagttaat taaagagaac ataaagcatt atgtatctta
     2041 tgcttttttg ggtgatgtta agagcatacc tgatatgcgc aaaacttatt acacgctgca
     2101 ttgcctgaat tgtggttacc tgagccttta tcgtacgtct gcggtgctga agtggttgga
     2161 gaaagacaaa gcgcaggatg gtgataatgg gtaatgtagc aagaaatctt ttcggtaatt
     2221 cagcaggtag tgtgtcgcat tctgagcgtg atgtgcttta tcatggtggc gatggcggca
     2281 gtagtggagg cggaatgtca gataaacttg aaaggcgaat tgagcggctc gaaggtgatt
     2341 tatcgctaac aagaaacgac cttgcgacgc ttgctgaacg cactacaaac ctctcaacta
     2401 aagccgatgt tggtgaggtg aaaggtgaac tcaaagcaga catagcacat ctgaaaggtg
     2461 atcttgaatg cgatattgcg aatctgaaag gtgagcttaa atctgataca gctaacctga
     2521 aagagcagct caaatcagac attaacagcc tgaagggtga gcttaccgaa gcgatggata
     2581 aacgctttga caagattatg gatgagatga atcggcggtt tgacaaggtt gatgataata
     2641 cgaagtggcg ttggagtggc attattgtgc cagtgtgcac aaccattttc acagcggcgg
     2701 ttgctgtatt tgttgctaaa tttgttggct gatgatccac aaattgacca catctctgtt
     2761 atttgttgtg gttggctgtg tttttgtgtg tctgtaagtc tttgataatt acctaactta
     2821 ttgatttttg tttgtgttta tggccgctct gcggcctttt ttcttttcac tgtcgaagag
     2881 tcaccgtaaa atcaacgcca tgacacttca gcagaacgga taccatgctg acacttgccc
     2941 gccaacaaca gcgacaaaat attcgctggt tattatgcct gtcagttttg atgctgctgg
     3001 cgcttctctt aagcctttgc gccggtgaac aatggatttc gccaggtgac tggtttactc
     3061 ctcgtggcga actgttcgtc tggcaaattc gcctgccacg tacgctggct gtattgctgg
     3121 ttggtgcggc gctggctata tccggcgctg taatgcaggc gttgtttgaa aatcctctgg
     3181 cagaacctgg actacttggc gtctctaacg gcgcaggcgt ggggcttatc gccgcggtat
     3241 tgcttgggca agggcaactc cccaactggg cgctagggct gtgtgcgatt gctggcgcgc
     3301 ttatcatcac tttaatactc ttacgtttcg cccgtcgtca tctttcgacc agtcggttat
     3361 tgctggctgg cgttgcatta gggattatct gtagcgcact aatgacgtgg gctatctact
     3421 tttccacctc tgttgattta cgtcagctga tgtactggat gatgggcggt tttggcggcg
     3481 tagactggcg gcaaagctgg ctgatgctgg cattgatccc cgtgttgttg tggatctgtt
     3541 gtcagtccag gccgatgaat atgttagcac ttggcgagat ctcggcgcgg caactgggtt
     3601 tacccctgtg gttctggcgc aatgtgctgg tggcagcgac cggctggatg gttggcgtca
     3661 gtgtggcgct ggcgggtgct atcggcttta ttggtctggt gatcccacat attcttcggt
     3721 tgtgtggttt aaccgatcat cgcgtattac ttcccggctg cgcgctggca ggggcgagcg
     3781 cagtgctgct ggccgatatt gtagcgcgcc tggcattagc tgccgcagag ctgcctattg
     3841 gcgtggtcac cgcaacgttg ggtgcgccgg tgtttatctg gttattgtta aaagcaggac
     3901 gttagccgca aaaagacggt ctatgattaa aagctaagat ttttactgac cacacccagg
     3961 agaaacgatg caagattcca ttctgacgac cgtagtgaaa gatatcgacg gtgaagtgac
     4021 cacgctggag aagtacgccg gtaatgtgct gttgattgtc aatgtcgcct caaagtgtgg
     4081 cttaacgccg caatatgagc agttggagaa tattcagaaa gcctgggccg atcgaggttt
     4141 tgtggtgctg ggattcccgt gcaaccagtt tctggaacaa gaaccgggca gcgatgaaga
     4201 gattaaaact tactgtacca ccacatgggg ggtgacgttc ccgatgttca gtaagattga
     4261 agttaacggc gaaggacgcc atccgctgta tcaaaaattg attgccgcag cgccgaccgc
     4321 agtcgcgccg gaagagagcg gattctatgc ccgtatggtc agcaaaggtc gtgcaccgct
     4381 gtaccctgat gatattttat ggaattttga gaaattttta gttggcaggg acggattggt
     4441 catccagcgt ttttccccgg atatgacgcc ggaagacccc attgtgatgg aaagcattaa
     4501 actggcgttg gcaaaataat gtctattgtg atgcagttac aggatgttgc ggaatctacc
     4561 cgcctggggc cactttctgg cgaggttcgg gctggggaga tcctgcacct ggtggggccg
     4621 aatggcgcgg gtaagagtac cttactggca cgaatggccg gaatgaccag cggtaaggga
     4681 agtattcagt tcgcggggca accactggaa gcatggtccg caacaaaact cgcgctgcat
     4741 cgcgcctatc tttcacaaca gcagacgccg ccgtttgcaa tgccggtctg gcactatctg
     4801 acactgcatc agcacgataa aacgcgtacc gaactactga atgatgtcgc aggggcgctg
     4861 gctcttgatg acaaactcgg acgtagcacc aatcaacttt ccggcggtga atggcaacgc
     4921 gtacgtcttg ctgcggtggt gctgcaaatc acaccacaag ccaatcccgc aggccaattg
     4981 ctgcttcttg atgagccgat gaacagtctt gatgttgcgc aacaaagtgc gttagacaaa
     5041 attctgagcg ctctgtgtca gcaaggactg gcgattgtga tgagcagtca cgatctcaac
     5101 cacacattgc gtcatgcgca tcgggcgtgg ttgctaaaag gtggaaaaat gctggccagt
     5161 ggacgcagag aagaggtgct cacgccgcca aatctggcgc aggcctatgg gatgaatttt
     5221 cgccgtctgg atatcgaagg tcacagaatg ctgatttcga ccatctgatt tcattagtat
     5281 cttgaaaatc gggtaattac gacgctaaat taatgcaaga ataaaaaaca gaggaaccgt
     5341 acggaatgcg tttctgcctt attttgatca cagcactgtt tctggccggg tgtagccacc
     5401 ataaagcacc gccgccaaat gccagacttt ctgattcgat taccgttatt gccggtttga
     5461 acgaccagct acaaagctgg catggcacgc cgtatcgtta tggtggcatg acgcggcgcg
     5521 gtgtggactg ttcgggattt gtggttgtaa cgatgcgcga tcgtttcgat ttgcagctgc
     5581 cccgagaaac caaagaacaa gcctctatcg gcacgcaaat tgataaagac gagttgctgc
     5641 ctggtgacct ggtctttttc aaaacgggtt ccggacaaaa tggtttgcat gtaggtattt
     5701 acgataccaa caaccaattt atccacgcct ctaccagcaa gggagtgatg cgttcctcac
     5761 ttgataatgt ctattggcag aaaaatttct ggcaggcgag acgaa
//
LOCUS       DAESHI010000188         5640 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.191, whole genome shotgun sequence.
ACCESSION   DAESHI010000188 DAESHI010000000
VERSION     DAESHI010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.191"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="KW011_004112"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="KW011_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HBH7662309.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="KW011_004113"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="KW011_004113"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HBH7662310.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="KW011_004114"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="KW011_004114"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HBH7662311.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="KW011_004115"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="KW011_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBH7662312.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1401 a         1352 c         1529 g         1358 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcttggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcggcggggt tctcctgaga
     5521 attccggcag agaaagcaaa aataaatgct tgactctgta gcgggaaagc gtattatgca
     5581 caccccgcgc cgctgagaaa aagcgaagcg gcactgctct ttaacaattt atcagacaat
//
LOCUS       DAESHI010000189         5617 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.192, whole genome shotgun sequence.
ACCESSION   DAESHI010000189 DAESHI010000000
VERSION     DAESHI010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.192"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..513)
                     /locus_tag="KW011_004116"
     CDS             complement(<1..513)
                     /locus_tag="KW011_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBH7662313.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(759..1211)
                     /locus_tag="KW011_004117"
     CDS             complement(759..1211)
                     /locus_tag="KW011_004117"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH7662314.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="KW011_004118"
     CDS             complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="KW011_004118"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBH7662315.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="KW011_004119"
     CDS             complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="KW011_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HBH7662316.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3091..4803
                     /locus_tag="KW011_004120"
                     /pseudo
     CDS             3091..4803
                     /locus_tag="KW011_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="KW011_004121"
     CDS             complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="KW011_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HBH7662317.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(5494..>5617)
                     /locus_tag="KW011_004122"
     CDS             complement(5494..>5617)
                     /locus_tag="KW011_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH7662318.1"
                     /translation="IAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLEGSGLS"
BASE COUNT         1339 a         1467 c         1459 g         1352 t
ORIGIN      
        1 cgagccgcta tgaaccaaca gttgcagatg ttgtgcatcc agacctgcca gtgcaaacag
       61 ttcagcgtcg atgatttgat caacttgttg cagttcagcg aaatgattgc cgccaccgat
      121 agagccaagc gagctgcgcc acggatgctg tgcaaatgct gacggcaggt tttcctccag
      181 ccagctttct tcagcaacgt catccaaagc tgataatcgc ttttcaaact tatcggcgtt
      241 gtatttgcga gcgagaatat ctgtttgcca tagcgccatg ccgcagccga tatcattgcc
      301 gaccagtgct gggtaaaaac ggccaacaga gaagaacgct gcgccaatcg ggtagccgcg
      361 tccgggatgt aaatctggca tccccactac gcgctgcatg ttgggtaaat ttgccgtggt
      421 gtgtaattgt tggatcgcta aactctcgat ccacaggtca tcagatgcga tggtaaatac
      481 cgcatcggat aagggacgaa tatatttgcc catgtaccat tccgtaaata gactcgtcat
      541 acttcaagtt gcatgtgctg cgactcgaat tattgagagt aacaattaaa aaatcaggaa
      601 atggcagacg aatccagggc aagaaaaaaa gaattaatcc tggaaaggtc tgcaaagagt
      661 cattgataaa ggatttgtca tgatttacct ccttgcagtt tgtggaataa gaaaacgcgc
      721 acatactagg cgggcgttat tttcattgca agctggattt aatgttgcgg tttatattgc
      781 atataaaaat tagtaaacca cgctccccgg cattcaacgt gctgctgctt aactgtctga
      841 aaaccataac gttcaaaaaa gggttttgcg gttatgctgg catctaccgt aagttcggat
      901 tcagacttaa taaatggttt taacaaggcg ctggcaaccc cgcggcgggt gtattcaggg
      961 tcaacaaata acatatcgat ataatgttca atgcgggaaa taaaaccaac cggttgtgca
     1021 ttaatgaccg caacccacac ttgtgatttc gcgagtttct ccttccagcg agattcgtca
     1081 atctgcgccc aggcggcaat ttgttgtgga gaataatgct gactggcggt catcgtaacc
     1141 gctctaatga aaatagcgca tagttgctga aaatcgccag gctgatagtt tcttatttgt
     1201 atgttattca taatcccagc accagttgtc tttccatttg cggatcaagc aacgtcacat
     1261 gcagccccac cagtcgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     1321 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgagtg gtttgctgaa
     1381 aatcgtcgaa ctttaatttc accccctggc gggcaatcag taaatcaggt tttacctttg
     1441 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     1501 ggtggatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgcaac cgttcgctgt
     1561 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     1621 agcgtttaag cagcatcacc agatcacact tttgtacatc accgcaggtt cgtagcccca
     1681 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gatttttgcc agtggtaagg
     1741 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctggccgttg ggtttattca
     1801 tgtcggaggc gattttggcg agaaacttta cgggtgccac gcccgcagac gccgtcagtt
     1861 gcagctcgtt gaaaatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     1921 aatggacgct atcggtgacg tcgagataag cctcatccag tgacaacggt tcaatacgcg
     1981 aggtgtagcg cgagaagatt tcgcggatat gatttgaggc ttctttgtag gcgtcaaagc
     2041 gccccggaag caaggtgaga tgcgggcata atttgagcgc catccctgtc ggcatagcgc
     2101 tacgtacgcc aaatttacgc gcgggataat tggcggtact gatcaccccc cgacgttcgc
     2161 ggctgccgcc aatagcaata gggatatcgc gcagggcggg attgtcgcgc atctccaccg
     2221 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     2281 tggtaaagta tacagtgatt tcagagtttg agaaatgcgt aaagattcag catttaacgc
     2341 gttcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     2401 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     2461 atctcaatgc gccggttgcc cgcgctttgc ggatttttgg aatccagcag catctggtcc
     2521 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     2581 cgagccgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtaa
     2641 gccatcgcat cggtatgccc ggtaataata attttgttat cgagcgagtc gaaaactggc
     2701 gcgagctcca ccagcagcgt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     2761 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
     2821 tccagattcg cttccatatg cgcatcacgg gcaatggtgg tgatactggt tgccagctcg
     2881 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattgac gtcgcgcgct
     2941 tttttctccg tttcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
     3001 attttattta acggcgacag cccgccaccg ttaaaaatcg actgcccgtg cagcgcggcg
     3061 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat tgtcgtcacc
     3121 aaaggggccg agcgtatctc cgaggtttct gcccgcttta ccctggacgc gatgcccggt
     3181 aagcagatgg cgattgacgc cgatcttaat gccgggttga tcaatcaggc gcaggcacaa
     3241 acccgacgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
     3301 aaatttgtgc gcggggatgc tatcgccggg atgatgattc tggcgatcaa cctgatcggc
     3361 ggcgtctgta tcgggatctt caaatacaac ctgagcgccg acgccgcctt ccagcagtat
     3421 gtgctgatga ccatcggcga cgggctggtg gcgcaaatcc cttccctgct actctccacc
     3481 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgt
     3541 aaccagttgc tggcaagccc atcggtgctc tacaccgcta ccggaattat gttcgtactg
     3601 gcggtagtgc cgggaatgcc acatctgccg tttttgctgt tcagcgccct gcttggcttt
     3661 accggctggc ggatgagcaa acgcccgcag gcggcggaag cggaagagaa aagtctcgaa
     3721 acgctgaccc gcaccatcac cgaaaccagc gagcaacagg tcagttggga aaccattccg
     3781 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcc
     3841 cagggcaacc cgctcaccca gcgtattcgc ggcgtgcgac aggtgatatc cgacggcaac
     3901 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gccttaagcc cagccagtac
     3961 gctattttca tcaacggcat taaggctgat gaagcggata ttccggcgga taaactgatg
     4021 gcgctgccct ccagcgaaac ctacggcgag atagacggcg tgctggggaa cgacccggcg
     4081 tacgggatgc cggtgacctg gatccagcct gcgcagaaag cgaaggcgct gaatatgggg
     4141 tatcaggtga tcgacagcgc cagcgtgatt gctacccatg taaataagat tgtgcgcagc
     4201 tatattcctg atttatttaa ctatgacgat attacgcagt tgcataaccg tttgtcgtcg
     4261 atggcaccgc gcctggcgga ggatttaagc gcggtgctca attacagcca gttgctgaaa
     4321 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
     4381 gtgctggtcg ccagtagcgc ggtgaccaaa gatcatattc tgctggcagc cgatgtgcgc
     4441 ctggcgctgc ggcgcagcat tacccatccg ttcgttcgca agcaggagct gacggtgtat
     4501 accctgaata atgagctgga aaatctgctg actaacctag tgaatcaggc gcaacagggc
     4561 ggtaaagtga tgctcgacag cgtgccagtg gacccgaata tgctcaacca gttccagagc
     4621 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagacccggt gctgctggtg
     4681 ccaccgcagc tgcgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
     4741 gtgctgtcgt ataacgaagt gccggacgag ctggagttga agattatggg ggcgttgatg
     4801 taagtatccg gcattggtgc acaactgccg gatgcggcgt aaacgcctta tccggcctac
     4861 cgttcaggca caagtttgta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
     4921 cataattata aaatgatacg ctcgccagca tttatatccg ttacgtcccc cgcccaatcg
     4981 atgggatata accctcgcgc gacatcgcga tggaacgttg agaatggcca atcactcact
     5041 tgctttaccc aaccatgctt tactggattt atataaatat aatcaacatg atgcctataa
     5101 tctttggtat tgcggatggc gtgctcccaa aaacgtggtt gccagatatt tttcaatcca
     5161 caagcatggg taaattgctt tttaatttcc cgccagcgag aggaaaaatc atcatcgcct
     5221 tcaggtaatg tccatatgca atgcatatgc tctggcaaaa cgacccaggc gttgatttca
     5281 aaaggcctgt ctcgcttaac tttaataatg gcgttacgga gcatctggta ctgggtggtc
     5341 aagagttggc ttcgacgatt gcgtaaattc accgtgaaaa accaggttcc ccccttgatg
     5401 taataacgac gatattcaga catggcgact cctttttctc aacggtaaga agaaaaggaa
     5461 aggtcggcaa tgaggtgatg gaacagggca agattacgaa agcccgctcc cctcaaggac
     5521 tgacgccaga tagtttctgt ccatggctgc ttttcgcatc ttacgtctta accctgcctt
     5581 gaatacctta tcattcgtca aaatattaat agcgatg
//
LOCUS       DAESHI010000190         5614 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.193, whole genome shotgun sequence.
ACCESSION   DAESHI010000190 DAESHI010000000
VERSION     DAESHI010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.193"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            12..428
                     /gene="soxR"
                     /locus_tag="KW011_004123"
     CDS             12..428
                     /gene="soxR"
                     /locus_tag="KW011_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HBH7662319.1"
                     /translation="MAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRYVAII
                     KIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRDELDG
                     CIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            974..2323
                     /gene="ghxP"
                     /locus_tag="KW011_004124"
                     /gene_synonym="yjcD"
     CDS             974..2323
                     /gene="ghxP"
                     /locus_tag="KW011_004124"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HBH7662320.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2475..4124
                     /locus_tag="KW011_004125"
     CDS             2475..4124
                     /locus_tag="KW011_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HBH7662321.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4278..5569)
                     /locus_tag="KW011_004126"
                     /pseudo
     CDS             complement(4278..5569)
                     /locus_tag="KW011_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT         1255 a         1271 c         1457 g         1631 t
ORIGIN      
        1 cccccggcga agtggcgaaa cgcagcggtg tggcggtatc ggcgctgcat ttctatgaaa
       61 gtaaagggtt gattaccagt atccgtaaca gcggcaatca gcggcgatat aaacgtgatg
      121 tgttgcgata tgttgcaatt atcaaaattg ctcagcgtat tggcattccg ctggcgacca
      181 ttggtgaagc gtttggcgtg ttgcccgaag ggcatacgtt aagtgcgaaa gagtggaaac
      241 agctttcgtc ccaatggcga gaagagttgg atcggcgcat tcatacctta gtggcgctgc
      301 gtgacgaact ggacggatgt attggttgtg gctgcctttc gcgcagtgat tgcccgttgc
      361 gtaacccggg cgaccgctta ggagaagaag gtaccggcgc acgcttgctg gaagatgaac
      421 aaaactaaag cgccacaagg gcgctttagt ttgttttccg gtctttgtct ttctctctat
      481 cccgctggta cacaggaggg tttcccccga cgtcaacaca ccccattcga gcacgtggtg
      541 gaggttccgg ttggtgttga tgctttaatt gtatgtcacc ggcatttctt cgccagtgta
      601 aaagtatact ttttatccgc aatatttttg tcatctcaga cgatttttta tcgcaatcct
      661 gaacggtata cggctcgata acgctgcaat cttgcgcacc gacgataacg tttgcgcatc
      721 aattgcctgg tttttcatcg tcaagacaat aaaagagaaa aaagcagcaa acttcggttg
      781 aaaaagccgc tatgatcgcc ggataatcgt ttgctttttt taccacccgt tttgtatgcg
      841 cggagctaaa cgtttgcttt tttgcgacgc agtaaattgt cgcaaacctg gagcaggaag
      901 ataacgtttc gctggcaggg gattgtccgc cacgcatctt gacgaaaatt aaactctcag
      961 gggatgtttt cttatgtcta cgccatcagc gcgtaccggc ggttcactcg acgcctggtt
     1021 taaaatttca caacgtggaa gcactgtccg tcaggaagtg gttgccgggt taacaacgtt
     1081 tctggcgatg gtctactcgg tcatcgtcgt tccaggtatg ttgggtaaag caggcttccc
     1141 gcctgcggca gttttcgttg caacctgtct ggttgccgga ctcggttcta tcgtgatggg
     1201 tctgtgggct aatctgccgt tggcgattgg ttgcgccatc tccctgacgg cgtttaccgc
     1261 attcagcctg gtgctggggc aacatattag cgtacctgtc gcgctgggtg ccgtgttcct
     1321 gatgggtgtg ctgtttacgg taatttctgc cacgggtatc cgtagctgga ttttgcgcaa
     1381 cttgccgcac ggtgtggcgc acggcacggg gatcggtatc ggtctgttcc tgctgctcat
     1441 tgccgctaat ggtgtcggtc tggtgattaa aaacccgctt gatggtctgc ccgttgcgct
     1501 gggtgatttc gcgaccttcc cggtgattat gtcactggta ggtctggcgg tgatcatcgg
     1561 cctggaaaaa ctgaaagtcc ctggtggcat tctgctgacc attatcggta tctcaattgt
     1621 cggtttgatc ttcgatccta acgtccattt ctccggcgtt ttcgccatgc cttcattgag
     1681 cgatgaaaac ggcaattcac tgattggcag cctggacatt atgggcgcgc tgaatcctgt
     1741 agtcctgcca agcgttctgg cgctggtgat gacggcagta tttgatgcca ccggaactat
     1801 ccgtgccgtc gccggccagg cgaacctgct ggataaagat gggcagatca tcgacggtgg
     1861 gaaagcactg accactgact ccatgagcag cgttttctct ggcctggtgg gtgctgctcc
     1921 ggcagcggta tacatcgagt ctgcggcggg tacggcggcg ggcggtaaaa ccggtttgac
     1981 ggctatcacc gttggcgtgc tgttcctgct gattctgttc ctctctccgc tctcttacct
     2041 cgttccgggg tatgcaacgg ctccggcgct gatgtacgtt ggcctgctga tgctgagcaa
     2101 cgtggcgaaa atcgactttg ctgattttgt tgatgcgatg gcgggtctgg ttacggcggt
     2161 attcatcgtg ctgacctgta acatcgtaac aggcatcatg atcggcttcg cgactctggt
     2221 gattggtcgt ctggtttccg gcgaatggcg caagttgaac atcggtacgg tcgttatcgc
     2281 cgtggcgctg gtgaccttct atgcgggtgg ctgggctatc taatctttca tcggattctg
     2341 aaaacgggtg gcaatggctg cccgttttta ttttctccgc acattgtgtg agcttttgcg
     2401 atattctgaa aaaatgagaa ttcaggcata acgcctttct cagatcataa aaaaacatcg
     2461 caaacaggga acgcatggaa atcttcttca ccatactgat aatgaccctc gtggtctcac
     2521 tgtccggggt ggtcactcgt gtcatgccct ttcagatccc gcttccgctt atgcaaatcg
     2581 ccatcggtgc gctactggcg tggccgacgt ttggtttgca tgtggagttt gatcctgaac
     2641 tctttttagt cttgtttatc ccgccgttgc tgttcgctga tggctggaaa acgccgaccc
     2701 gtgaatttct cgaacatggt cgagagattt tcggcctcgc gctggcgctg gtggtggtca
     2761 ccgtggtcgg cattggcttc cttatttact gggtggtgcc gggcattccg ctgatccccg
     2821 cctttgcgct ggcggcggta ctttctccga ccgatgctgt ggcgctctcc gggattgttg
     2881 gcgaagggcg catcccgaaa aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg
     2941 acgcctccgg cctggtgtcg ttgaagtttg ccgtggcagt ggcgatgggg acgatgatct
     3001 tcaccgtcgg cggtgcgacg gtcgaattta tgaaagtagc cattggtggt attcttgccg
     3061 gttttgtggt gagctggctg tatggtcgct cgctgcgatt cctcagccgc tggggcggtg
     3121 atgaacccgc gacgcagatc gttctgctgt tcttgctgcc attcgcttct tatctgattg
     3181 ccgaacatat tggcgtttcc ggcatcctcg ctgcggttgc cgccgggatg accatcaccc
     3241 gctccggtgt gatgcgccgt gcgccgctgg caatgcgcct gcgcgcaaac agcacctggg
     3301 cgatgctgga atttgtcttt aacggcatgg tgttcctgct gttaggtctg cagctgccgg
     3361 gtattctgga gacgtcgctg atggcggcag aaatcgaccc taacgtcgaa atctggatgc
     3421 tgtttaccga tattattctg atatatgcgg cgctgatgct ggtccgtttc ggctggctgt
     3481 ggacgatgaa aaagttcagc aaccgcttcc tgaagaagaa gccgatggag tttggttcgt
     3541 ggaccacacg agaaatcctg atcgcgtctt tcgccggggt gcgtggggcg atcactctgg
     3601 ccggtgtgct ctctatcccg ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg
     3661 agctggtgtt cctggcggct ggcgtcattc tcttctcgct gtttgtcggc gtggtgatgt
     3721 tgcctattct gctacaacac attgaagtcg cggaccattc gcaacaattg aaagaagaac
     3781 gtattgcgcg agcggcaacg gcagaagtgg cgattgtggc gatccagaaa atggaggagc
     3841 gtctggcggc ggataccgaa gagaatatcg ataaccagct gcttacggag gtcagttctc
     3901 gcgtcattgg taacctgcgt cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc
     3961 aggaagagaa ccttgagcgt cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac
     4021 tgtaccacct gcgcgccacg cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg
     4081 atctcgattt gcttgaagcg ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa
     4141 ccaggcccga ggtaatctga aaaaggtgca atcttttgca cctttaacag gttagctcgg
     4201 gctaacattc atgattctaa aacaaataag ttatctctct tgtaaagtca attcattggc
     4261 tgttcttcga cttaacctca atctgcgtgt tgcataatat ctgcagtaat cttcatagcg
     4321 actgcattaa tattatcctc agtttgtgac atgccagtat gagaggtaca atcttcttca
     4381 aagaatttct gtagtgttac cagggtatca ctatcaatgg ggatattttc ctccccacgg
     4441 agtatggaaa tgcccattcc ctggacgcta gcctcatcaa aattaacccg atagagacgg
     4501 gtattaaaca tacaggcttt tgttaaaata gaacagttga aatggcagtt gcttaaattg
     4561 gcattattaa aattaacatt agccatatta gagtcggtac atataaccat ttcgaggaat
     4621 gtatttgtta gccacgtaca aaagagtttg ctatcaatta agtttatatt ctggaggatt
     4681 gctttgttca gattgacgcg agacagatca gcatgttcaa agttaattaa atcgagttta
     4741 gagaaactaa ggtcggcttt aaataaatta acatatggcg ctattacttt ataaagattt
     4801 gcctcagcca tgtacgccat ataaaaattt gaatagtcaa gaaaggtatt tttaatattg
     4861 gctctattca ttgctgcatt gtagaattga acatgcataa cggagcatgc atttagagtt
     4921 ccgtcggcca tttttatctg agcgaaattt gagtcatcga gaatacaatt atcaaaaagt
     4981 atttttccat tatggcgtaa taaaaattcg ttttatacat attacagtcc tggaaaatag
     5041 cgttttgtag tctgcaacct gcaaaattcg aattggataa atcttcatct ttaatcacga
     5101 tacctttcag gctaaggtgt gaaaaatcgt aaccaccatt tactgtctgt ggaatttcga
     5161 tagtattatt atggacggcg acattattta taaaatatac caccttaatg gatacttcct
     5221 tttctttact aaggtaaact cttctcgtaa ttctatcgtc acatttatta tttgcatata
     5281 aagctatatc tataaaatgt ttatgtattt tcctgtccgt ttccttcgtg atttttcctg
     5341 atggtaaact atcataaatt cttttcattt tctgaatcaa acctatcact ttttcatgat
     5401 catccatctt gaaaagagag agggctttat tgtcaattaa cgtgttttta tgatgtttgc
     5461 tgtaattata tgattttgtt ttctttgtat gtgatgaact tgtgaaagag tggttatcgt
     5521 taatctggca aagagagaaa aacatattat ttaaaccatt atagcgcata aaatatcctt
     5581 ataattaaca agaaagaaaa ggcatattct ctgc
//
LOCUS       DAESHI010000191         5609 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.194, whole genome shotgun sequence.
ACCESSION   DAESHI010000191 DAESHI010000000
VERSION     DAESHI010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.194"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(1..1133)
                     /locus_tag="KW011_004127"
                     /pseudo
     CDS             complement(1..1133)
                     /locus_tag="KW011_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1133..1315)
                     /locus_tag="KW011_004128"
     CDS             complement(1133..1315)
                     /locus_tag="KW011_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662322.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            complement(1327..3060)
                     /locus_tag="KW011_004129"
     CDS             complement(1327..3060)
                     /locus_tag="KW011_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBH7662323.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(3057..3551)
                     /locus_tag="KW011_004130"
     CDS             complement(3057..3551)
                     /locus_tag="KW011_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBH7662324.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3673..4023)
                     /locus_tag="KW011_004131"
     CDS             complement(3673..4023)
                     /locus_tag="KW011_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBH7662325.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4066..4530)
                     /locus_tag="KW011_004132"
     CDS             complement(4066..4530)
                     /locus_tag="KW011_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBH7662326.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(4527..5024)
                     /gene="rrrD"
                     /locus_tag="KW011_004133"
     CDS             complement(4527..5024)
                     /gene="rrrD"
                     /locus_tag="KW011_004133"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HBH7662327.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(5024..5239)
                     /locus_tag="KW011_004134"
     CDS             complement(5024..5239)
                     /locus_tag="KW011_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBH7662328.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT         1255 a         1623 c         1369 g         1362 t
ORIGIN      
        1 gggacgcgga ttcatatctt ccaggtcgcg gcagtcattg gaagagtaaa ttccccagtt
       61 gatcccggtg gcgtaggctt caaaacggga cttcatatcc ccgcgcagta acgccccggc
      121 gttaaatttg gcgtaataaa cgccctgctt actttttcgt accagtccgg tgttgatccg
      181 ctgttcgatg cgggtcagat acggcaccag tgaatagttg ataaatccca gccccagctc
      241 ttcgatattg ttgaaggtgg cgcgatcggt gttctgcacc atgtgcaacg gcacccggaa
      301 cagacgacag atttcttcca gctgaaactt gcgggtttcc aggaactgac tgtcctcggc
      361 gttcagcgcc atcgacttcc agtccagccc catttccaga atcatcgggc ggtgagcatt
      421 gccaagcccc gtgtgacact cctcaaaatc tttcttcaga cgctcgtaag catccggcgt
      481 gagcttttgt tccgtacgca acacaccgga tgtcaccgca ccattactaa acaacctggc
      541 accgtgctcc tcggttgccg ctgccagtga aatggcctca cgcgcatacg caatgggatt
      601 cagcccgaca agtccatcca gcgtcagggt gcgcacatgc cagatttcat tctgggtcaa
      661 cacatccacg gaaccatccg gaaacgtcac ctgataaacc ggctgccact ggctgttcag
      721 cttcggttcc acacagcccg gatctatcgg aagaagctcc accacttctc ccagtgcctt
      781 taccttgtag gcgtaaaaat tcccccgtag acacaggcag acaataacca gctcccagaa
      841 ttcctgcggt gtcatgtagc cattaggttt tgccgaaacc aacttatgca gtcgttcatc
      901 caccgcccgt gttttcaggg taccgctgat tttgtagaga ctgcagggca gcataccaac
      961 agactctgcc agcaccctga cgcaagaata gaccgccgtc aggcgcatgg cccgctggct
     1021 actgatccgc tttccggtat aggtgtcgta tgacagcccg atagcatcag cagttctgct
     1081 ggcgtggtca ccggcacatc gctttttcgt tgaaataatc ccgaaaagaa cactatttac
     1141 ctccgccgac agactgccgt gtacggtcga gatatcgcgc caccagccac gaccagaaca
     1201 ggcacagcgc cccggcaaca acaaaacccg ccgggggata aatcagccag gcaccatacg
     1261 ccagcaaaag cgcacccagc acgcccacca gaggcgcgag aatcagcatg atcataatta
     1321 cctcagttaa agcgagcgga tcccgtagga ctcaatgtgg tcagacagcg tgtcttcttt
     1381 ctcgtacagc atggctctgc caaccgccat aatcagcgca actgcaccat cgattttgtt
     1441 ttccgcctgc tctttgacgg gtttcaccac atcatcgtta cccggcatgt ttttgccgac
     1501 cacgttgccg atacaccagg tcatgatggg attgccgtca tgatgaaaac gtcccgattc
     1561 aatcgctgct tccagctctt tcatcggatc ggacatattg gtgaagttct ggacgatagt
     1621 gacgggattc aggtcttcat cagcaaggtc atgtgacaac ccggtcgccc cgaaggggtc
     1681 gatgggtgac tcactgaccg ggctgatttt gttcgccgct ttggcctcct cgaggatgta
     1741 gcgataatcc acctccgcac catcggtaac ggtcagaacg cccatttcca cccatttctg
     1801 aaagcgttcg gctgtccgtc gatcttcatt tttctcgacg ctgtacaccg tgtcatacgg
     1861 tacccagaaa cgcggggcca cactgtagta atgcgtttta ccgtcaatct cgcgggtata
     1921 aagtcgcgcc atgctgttca tatccagctt acgcgccagg tcaaaggcca gaatgcacgg
     1981 ctgcccctcg aactgctcaa gggtcagtga tttatcctcg cagctctgcc agctcaccaa
     2041 gttgaaatac gccgaacgcg ccgacaccca gatattgagg tgttttgttt taaagacgtt
     2101 tgccagacgg gcgttatttt tcgcacgctg ctgctgactt aacaaaaatt cgcgataaac
     2161 cgacacgcca atatttggat tggctttttc cagcacctgc gggccggtcc agtcgtcacc
     2221 ttcatcaacg gtatagatga tcccgaacag ttcatcgtta ggcaccgagc cgttgagcat
     2281 ctcgatgact tcccgccgct tgtcgtagca cggcccctca atgttgtacc cggcggtggt
     2341 gatggcccac atcagtggct gacgtcgcgc ccccatcccg gtaagcattg tggtataaag
     2401 cgcatcggtg gcatgctcgt gatattcatc aaccacggca cagtggggtg atgaaccatc
     2461 acctgggttg ccgatcagcg gttcaaaccg cgcgccatcc tccggacggt tcatgtttga
     2521 ggcgttaacc tcaatcccga acgcttccgt cagcatgggt gtgcgtttac acatcagtcg
     2581 cgccgggcga aagacttccc acgcctgttt ctctgtcgtg gcaccggaat acacttccgc
     2641 gccaaactcg ttatcacagg caaaacaata cagggcaaca ccggcagaga ttgctgattt
     2701 gccgttctta cgggggattt cggtgtacac ctcccggaag cggcgcaacc gggtgccttt
     2761 attgacccag ccaaacgcac agcagatcac aaatagctgc cacggctcca gcgtgatggg
     2821 catccgtttg aatgcccact cccccttggt gtgcggcaac agctgaataa atttcgcggc
     2881 ccgttcagcc aggtccttgt cgaagcggta acgaaacgac ttactttttt ccgccatcag
     2941 gtcatcaaga tggcgctggc aggcctgaat cacaaactgg caggcaacaa tctttccgcg
     3001 cacgacatcc cgggcatact gatttgcagc atttacgttg gggtaagatt tccggctcat
     3061 gattcgataa ttttcagaaa cgggttagtg gctttcttct gccccgccag gccaatcaga
     3121 cgctggcggc tgctggggtc gagtccgagc attgcccccg tgctgctcat ctcggactcc
     3181 tgttcttttt tggcggtcag ctccggattt ttgaccctgc cgcccattgc accggtgatg
     3241 gtgttgccct gtatggcaat atttttcacg gcacgtcgcc agaactcata ggccacgcac
     3301 caccgctcaa gtaccgccag gtcagtcacg cacagcaggc cctgaccgca gagttctttg
     3361 gttgtcagtt gccacatgat cgtggcgaga gggagatctt cttcagcgaa ccactctggt
     3421 ggctcaacac ctttgatggg cgtaaaaaca ggttcatctt tgttcagggc tcgcttgccg
     3481 gggtttccgg ccagcgcctt gcgcgccgtt ggcttggggc gacgcccgga acgccccgcc
     3541 gttccagcca tatgcggcac tcctggttaa atttcatttt tcgcgggtat aaaaaaacga
     3601 tggggcgggc agtccggaag acgtcaggtc acagggattt gccccgcccc tccctacaag
     3661 tgagaataat tatcaccgga ttcgttcgcg cgctgttttc gctttatggc agggccagca
     3721 cagactctgc aggttgctgt ctgcgtctgt tccgccatgc gctttcggga tgatgtggtc
     3781 gacggttttc gcctcgctca ccacaccgac acgcagacac aactgacaca gacctttatc
     3841 gcgcttcaga atacgggcac gaatcaccgt ccattttgag ccatagccac gctggtggcg
     3901 gctcagtccg cgctggtgct gcacccagcc ttcaccgcga tgtttatcgc agtaacccga
     3961 actgtctgtg gttgtacctg cacatccacg cttacggcag gcacgtggga ttagtgatgg
     4021 cataaatacc tcataccctg cgaaatgttt accacgataa aaaggctact taatgcactg
     4081 agtgcggata tactcctgtg ccccttccag ttgcatctgc atcgtcatca gccgctctct
     4141 gagggtgaaa taatcccgtt cagcggtgtc tgccagtcgg gggctggttg cattatccac
     4201 gccggaggcg gtggtggctt cacgcactga ctgacagact gctttgatgt gcaaccgacg
     4261 acgaccagcg gcaacatcag cgcgcagagt ttcattttca gctttcgcat cagctaactc
     4321 cttcgtgtat ttagcatcga gtgcattaac atcacgctgt ctctgctgca tgtcagtaat
     4381 ggttgcgttc gccagcttca gttctcttgc gtttttgtcg cgctgctctt tgtaggtaat
     4441 ggcgttatca cggtaatgat taacagccca tgacaggcag acgatgatgc agataaccag
     4501 agcggagata atcgcggtga ctctgctcat tgctgccccc acaaacagac ttcacgctca
     4561 atctcacgac gggtcatcag ccctttccat tgcttaccgc cagcatatgt ccagcgacgt
     4621 agctggtcac atgcgccttt gatatcgccc tggtttattt tgcgaagaag cgtcgatgtt
     4681 ctgaaattgc cagcgcccac gttgtaaacg aacgagtaaa gagcgccgcg cgttgtttcc
     4741 ggtatatcga ctttgatgta cgggttaatt tgtctggcga cagtggcaag gtctttattc
     4801 aggagggctt tgcattctgc ttcggtatac gttttaccga gcatgatgtc ttttccggtg
     4861 tgtccgtgac atacagtcca tacaccaatg atatctttgt atggtatgta gctgacacct
     4921 tccagaccat cgttaccacc tgggccagtg attaacacag atgctatagc aacagcccca
     4981 ccaccaatag cagctgcaac agccttgcgt aatgatggcg acattattca cctctcgcag
     5041 ccttacgctt gtcttctctt attttgaaat acagatttgt cagataagtc agaagcccca
     5101 gaagcagact tcccagcaca ccaatcgcag cccactgtga tggactgacc tgatccaacc
     5161 actgcaaaaa ccagtagccg gcactgccgg cggaggtgcc gtaggcaatg cctgttgata
     5221 ttttttccat ctgatacata tcccgccccc cgacagacct gtgctatcgg aaagaaaaaa
     5281 ggccatcagc aaaactctga tggcctgaat cacctttacc aatattgtat gaaaaaacac
     5341 gcacgactaa ttgacaataa ttttcatttc cattaaaaaa gatcgcgtag cattcttaat
     5401 tcatgaggaa ctttacccgc cagcaatctg agtagcgaaa gctgttcatc cccaatgttt
     5461 ttgctggcgg gtcctttttt ctacggtcct cctccccgga ggggggcatt tcattatttt
     5521 tctaatcgtt actggtgaga aggcggaaga aaaagccagt tcttcgcccc taaataacag
     5581 catgattcca gtccatgcaa aatctgatt
//
LOCUS       DAESHI010000192         5605 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.195, whole genome shotgun sequence.
ACCESSION   DAESHI010000192 DAESHI010000000
VERSION     DAESHI010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5605
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.195"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..609
                     /locus_tag="KW011_004135"
     CDS             <1..609
                     /locus_tag="KW011_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein"
                     /protein_id="HBH7662329.1"
                     /translation="PTITSGGNPSAFSLTPDGKLTAKNADISGSVNANAGTLNNVTVN
                     ENCTIKGMLEATQVRGDFVKAVSKSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQII
                     IPPIIFNGIAYDDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDM
                     PSGGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISIR"
     gene            680..1196
                     /locus_tag="KW011_004136"
                     /pseudo
     CDS             680..1196
                     /locus_tag="KW011_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            1261..2665
                     /locus_tag="KW011_004137"
                     /pseudo
     CDS             1261..2665
                     /locus_tag="KW011_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            2614..2766
                     /locus_tag="KW011_004138"
                     /pseudo
     CDS             2614..2766
                     /locus_tag="KW011_004138"
                     /inference="COORDINATES: protein motif:HMM:NF015371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            2743..2922
                     /locus_tag="KW011_004139"
                     /pseudo
     CDS             2743..2922
                     /locus_tag="KW011_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            3637..4344
                     /locus_tag="KW011_004140"
                     /pseudo
     CDS             3637..4344
                     /locus_tag="KW011_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            4344..4860
                     /locus_tag="KW011_004141"
                     /pseudo
     CDS             4344..4860
                     /locus_tag="KW011_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT         1572 a         1333 c         1504 g         1196 t
ORIGIN      
        1 cccaccatta ccagcggtgg aaatccatcg gcattttccc tgactccgga cggaaagctg
       61 accgctaaaa atgcggatat cagtggcagt gtgaatgcga acgccgggac gctcaacaat
      121 gtcacggtaa atgaaaactg tacgattaag ggcatgctgg aggcgactca ggtcagaggt
      181 gacttcgtta aagctgtatc caaatcattt ccgaaacagg ctggtacgtg gggtaacacg
      241 gaaacaccaa acgggacggt tacagtcacc ataagcgatg atcataactt tgaccgccag
      301 attattattc cgcccattat ttttaacggt atagcgtatg acgatccggg gagcggaaat
      361 aacccaggag gcacgcgata cacgggttat ggttttgaag ttcgcaaaaa cggcgtatta
      421 atcgcatcca gagaaactaa aggtgccatt cccggtagtt acagtgcagt tattgatatg
      481 cctagtggtg gtggtagcgt cactctggag tttaagattt tccagaaagg caatcagggg
      541 gcaggcaata tcaccgactg tacggtgatt gtgaccaaaa aagcggcttc cggcatcagt
      601 attcgttgaa atatttataa ccccaataaa tggcgtcagg aatgacgcct tttttattgc
      661 agaaaagcga gaggtaatta tgcgtaaagt ttgtgcagcc attttgtccg cagccatttg
      721 cctggccgta tccggtgcgc ctgcatgggc gtctgaacat cagtccacgc tgagcgcggg
      781 gtatcttcat gcccggacca acgttcccgg cagtgatgat ctgaacggga ttaacgtgaa
      841 ataccgttat gagtttacgg atacgctggg gctggtgacg tcattcagct atgcaggaga
      901 caagaatcgc cagctgaccc gttacagcga tacccgctgg catgaagatt ccgtgcgtaa
      961 ccgctggttc agcgtgatgg cggggccgtc tgtgcgcgtg aatgaatggt tcagcgcgta
     1021 tgcgatggcg ggtgtggctt acagccgtgt gtcgactttt tccggggatt atatccgcgt
     1081 aactgacaat aaggggaaaa cgcacgatgt gctgaccgga agtgatgacg gtcgccacag
     1141 caacacgtct ctggcgcact gacggtttca tcgtgggtgt cggttataaa ttctgattag
     1201 ccaggtaaca cagtgttatg acagcccgcc ggttcaggcg ggcttttttg tggggtgaat
     1261 atggcagtaa agatttcagg tgtactgaaa gacggcacag gaaaaccggt acagaactgc
     1321 acaatccagc tgaaagcaaa acgtaacagc accacggtgg tggtgaacac gctggcctca
     1381 gaaaatccgg atgaagccgg gcgttacagc atggacgttg agtacggtca gtacagcgtt
     1441 attctgttgg tggaaggatt cccgccgtca catgccggga ccatcaccgt gtatgaagat
     1501 tcccgacccg gtacgctgaa tgattttctc ggtgccatga cggaggatga tgcccgtccg
     1561 gaggcactgc gccgttttga gctgatggtg gaagaggtgg cgcgtaacgc gtccgcggtg
     1621 gcacagaaca cggcagccgc gaagaaatca gccagcgatg ccagcacatc agcccgtgag
     1681 gcggcaaccc atgcgactga tgctgcagat tcagcacgtg cagccagcac gtcagccgga
     1741 caggccgctt cgtcggctca gtcagcgtct tccagcgcag gaacggcatc aacaaaggcc
     1801 actgaagcct caaaaagtgc tgccgctgca gagtcctcaa aaagcgcggc ggctaccagt
     1861 gccggtgcgg cgaaaacgtt agaaacgaat gcggcagcgt cacaacaatc agcagccact
     1921 tctgcatcca ccgcgaccac gaaagcgtca gaagctgcca cctcagcccg ggatgcgtcg
     1981 gcttcaaaag aggcggcaaa atcatcagaa acgaacgcat catcaagcgc cagtagcgca
     2041 gcttcctcgg cgacggcggc tgcaaattct gcgaaggcgg ccaaaacgtc cgagacgaac
     2101 gccaggtctt ctgaaacggc agcgggacag agtgcctcag ctgcggcagg ctcaaaaaca
     2161 gcggctgcgt cgtctgccag cgccgcgtca acaagtgccg ggcaggcctc agccagtgcc
     2221 accgccgccg gaaaatcggc agaaagcgcc gcatcatccg cttcaacagc cacaacgaag
     2281 gctggcgaag ccactgagca ggccacggca gcagcgaggt cagcttccgc agcgaagaca
     2341 tccgaaacga acgcgaaagc gtcggaaacc cgtgcagaat cctcaaaaac ggctgccgca
     2401 tcgtccgcca gttcggcggc gtcatcggca tcatcggcgt ctgcttcaaa agatgaggcg
     2461 accagacagg cgtcagcagc gaagggcagc gccacgacgg catccacgaa ggcgacagag
     2521 gctgctggta gtgcgacggc agcagctcag agcaaaagta cggcggaatc tgcagcaacg
     2581 cgcgctgaga cagcggcaaa acgggcagag gatattgcat ccgccgtggc gcttgaggat
     2641 gcgagcacga cgaaaaaggg gatagtacag ctcagtagtg cgactaacag cacttccgag
     2701 tcactggcgg caacaccaaa agccgttaag gccgcgtatg acctggctaa cgggaaatac
     2761 accgcacagg atgcaacgac agcacagaaa gggataatcc agctaagcag cgcgaccaac
     2821 agcacgtctg aaacgctggc ggcaacgcca aaggcagtaa aagcagccaa tgacaatgct
     2881 gagaaacgtc tgcagaaaga tcagaacggt gcggatatcc ctggcaaaga cacctttacg
     2941 aaaaatattg gtgcctgccg tgccttcggt gggtcagtaa gcacaacaac aggaaactgg
     3001 acgactgcac agtttatcga gtggctggat tctcagggag catttaacca tccatactgg
     3061 atgtgcaagg gttcctggtc ttatggcaat aataaaatca ttactgatac tggctgcggt
     3121 aatattcatc tcgccggagc tgtcattgaa gtaatgggga taaagtcagc gatgacgatc
     3181 cgcattacca ctccgaccac atccagcggt ggcggaacaa ccaacgccca gtttacctat
     3241 attaatcacg gaacagatta ttcacctggc tggcgaaggg actataactc cagaaataag
     3301 ccaacggcat cagagatcgg ggcgctaccg tcaggtggaa cagcagtatc atcagttaat
     3361 ctggcttcaa aaggtcgggt agccgcgctg acagacaata cgcagggggc agcaggtctt
     3421 gagttatacg aggtgtataa caacggatat ccaacagcgt atggaaatat cattcacctg
     3481 aaagggatga cagccgttgg cgaaggtgag ttactcatcg gctggagtgg tacaagcggt
     3541 gctcatgctc cggcatttat tcgttcacga cgggatacga ccgacgcaaa ctggtcgccg
     3601 tgggcgcagc tttacacctc ggctcatcct cctgcagagt tttatccagt cggtgcacca
     3661 atcccgtggc catcagatac cgttccgtct ggttatgccc tgatgcaggg gcagactttt
     3721 gacaaatctg cttacccgaa acttgcagcc gcttatccgt caggcgtgat ccctgatatg
     3781 cgtggctgga cgattaaggg caagcccgcc agtggtcgtg ccgtattgtc tcaggaacag
     3841 gatggcatta aatcgcacac ccacagcgcc agcgcatcca gtacggattt ggggacgaaa
     3901 accacatcgt cgtttgatta cggcactaaa tccacgaata acaccggggc gcatacgcac
     3961 agtctgagcg gctctacaag ttcagctggg gcccaccaac atagtcagac tgggcccaga
     4021 acgaatagcg ggagtcaacc tactggaatg tttccggcag ggagtactca agttagcggg
     4081 actaatcagg ttggtatcag tggctcatta accagtggca cctcccaatg ggtaggtaaa
     4141 tcatcttcag aaggaaacca tacacactca ctgtccggca cagcagcatc tgcgggtgca
     4201 cacgcgcata ctgtcggtat tggtgctcat acgcactctg ttgcgattgg ttcacatgga
     4261 cacaccatca ccgttaacgc tgctggtaac gcggaaaaca ccgtcaaaaa catcgcattt
     4321 aactatattg tgaggctcgc ataatggcat tcagaatgag tgaacaagca cggaccataa
     4381 aaatttataa cctgctggcc ggaactaatg aatttattgg tgaaggtgac gcatatattc
     4441 cgcctcatac aggtctgcct gcaaacatcg tggcatctcg ttgaagatca tcggggtaaa
     4501 acggtttatg acgtggcttc cggcgacgcg ttatttattt ctgaactcgg ctcattaccg
     4561 gaaaatgtca cctggttatc cccggaaggg gagtttcaga agtggaacgg cacagcctgg
     4621 gtgaaggata cggaagcaga aaaactgttc cggatccggg aggcggaaga aacaaaaaac
     4681 agcctgatgc aggtagccag tgagcatata gcgccgcttc aggatgctgt ggatctggag
     4741 atcgcaacgg agaaagaaac cttgttgctg gaagcctgga aaaagtatcg ggtgttgctg
     4801 aaccgtgttg atacatcaac tgcaccggat attgaatggc cagtagcacc tatagggtaa
     4861 attcgtaatg attacctaaa tacgttattc ttttgttaaa aaagtgattt tgctataggt
     4921 agggagtatg ttcttggatt aagaataaaa aatcgccatg aaagagcttc atggcgagta
     4981 cataaacgta caaaatgtga tgaaagtaga gtggcgtaag ccactatatt aagatatgac
     5041 aatagcgaag aaaagtaaat aacattttat ggacgtttaa ttgttaatta taaaaactag
     5101 agtgcccgat attgtttatc ttgctttaaa ataatgtact caaaccaaat ttttgagcta
     5161 aaagctcaaa taatataaat gcaataataa tgtttaataa gacaattagc ttgctttgta
     5221 ttttcatttc ggaatactgt taagtaactt tgatgaccct aacaatagat cggaattttc
     5281 ttagagtcaa tggtcactgt ttttgcttgc aaagcaaatg ttttggtggt tataagttgt
     5341 cttaatatag tcacgcatgc aacttgttcc atgcggagat taaagattgg aatataacgc
     5401 gaagatggaa ttaaattaca tttgcaataa tcaatttatt attactcaat aattaccgat
     5461 attattattg taaattaatc attagagtgc aaaaagaaat gtaacggcaa aataagagga
     5521 cgatagctga gaaaaaagcg tgctttccca atctggggca aatcacgtct gaatgacacc
     5581 tgaaaacagg tggtagcctc aagaa
//
LOCUS       DAESHI010000193         5541 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.196, whole genome shotgun sequence.
ACCESSION   DAESHI010000193 DAESHI010000000
VERSION     DAESHI010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.196"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..1369)
                     /locus_tag="KW011_004142"
     CDS             complement(<1..1369)
                     /locus_tag="KW011_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HBH7662330.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWL"
     gene            complement(1576..1860)
                     /gene="ihfB"
                     /locus_tag="KW011_004143"
     CDS             complement(1576..1860)
                     /gene="ihfB"
                     /locus_tag="KW011_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HBH7662331.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(2020..3693)
                     /gene="rpsA"
                     /locus_tag="KW011_004144"
     CDS             complement(2020..3693)
                     /gene="rpsA"
                     /locus_tag="KW011_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBH7662332.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(3804..4487)
                     /gene="cmk"
                     /locus_tag="KW011_004145"
     CDS             complement(3804..4487)
                     /gene="cmk"
                     /locus_tag="KW011_004145"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBH7662333.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(4660..5424)
                     /gene="ycaL"
                     /locus_tag="KW011_004146"
     CDS             complement(4660..5424)
                     /gene="ycaL"
                     /locus_tag="KW011_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HBH7662334.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         1410 a         1450 c         1304 g         1377 t
ORIGIN      
        1 atagccattg ccaacgttca gcaatgttga tccacccttc tggcaaactc tttaaccccc
       61 agaaaagtaa agccaaagac cggtcggcaa gaaaccataa cccttgctcc aggattaacg
      121 gcccgcttaa atgcacaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca
      181 agggaattgc taatagattt gcaataaacg aggtcagact aatgccatga aatatgacga
      241 tttgcacggg cataagcaga agtgtgatac ccagttgcag atggatgagg gaaacaactg
      301 cacgcaatac cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca
      361 gtgccgcgac cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca
      421 tcagcaaaat tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac
      481 ttaacttaag cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca
      541 ttcccgtcaa ccaggcataa aaagcagcac agcagatccc accaattaat ggtatttgcc
      601 agtggatcca gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca
      661 acaccgcaaa agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc
      721 gcatgatatt tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa
      781 taaccgcatt ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac
      841 gcaggctaca attcggttca ataacacttg cctgcagaaa gcggccggtg agcggctgat
      901 gctgggcaat ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaacgg
      961 cacgaacttt gagtttcatt gaccacagtt gtccggcaca aaccgcttgc ggaagatatt
     1021 cgccatacag cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt
     1081 gaccgtaatg agtggtcatg ccgtcagtag cagtgatctc aacaattgca tcctgcgtcg
     1141 cgccagttaa ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga
     1201 aaaacagcaa cgtcagcgcg atataacgga cggttttaac aggtataaat gccagtacgc
     1261 aggcgaagag agtcagaaac gcaagggcta atgtcccagg caattggggc aaaatcagca
     1321 acggaaaaat tccgcaaatt atgcatacgt cgactgtcgt tattttcatg cactcctccc
     1381 tgtttatgga aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc
     1441 gaggcacatt cagaagaatt taacgagagg gtgaaaactg ctcaaaactc tgcgaggcag
     1501 cttccagttc ctgattagag aaatgaaaaa agcacccgac aggtgctttt ctcgcgttca
     1561 agtttgagta aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt
     1621 aaagtgagga acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc
     1681 ggtacgtggt gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc
     1741 gccctgcgca agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt
     1801 cttggcggga atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat
     1861 gattcctccg gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc
     1921 ggccgtagcc gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact
     1981 caacaaactt cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt
     2041 gaaagcttca gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc
     2101 gattgcatct ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt
     2161 tttacgatca acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccaaggt
     2221 agcgtcttca acgcggtcac gagatgcttc agaagcacgc aggtaacctt caacgccgtc
     2281 agccagttct acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc
     2341 gcctttcttg ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc
     2401 cagggagata cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc
     2461 ttttttgtat tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg
     2521 aaccaggccg tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt
     2581 gattttacct tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt
     2641 agctttgcac tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac
     2701 cataacttcc actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt
     2761 ccaatccatt tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca
     2821 gccgtagtca gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt
     2881 agcgatagct acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg
     2941 ttcgcggtcg aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct
     3001 cggatgctta acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc
     3061 cagatcaacg aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc
     3121 ttcctgcagg ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc
     3181 acgacgagaa acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag
     3241 ctctttgcct tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg
     3301 caggaacgca cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat
     3361 aacaccggta acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg
     3421 acgtttagct ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag
     3481 agcaacgtca acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg
     3541 ctcagccggg atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc
     3601 gatagcaaca acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga
     3661 ctcttcaaag agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg
     3721 tccacctggc tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac
     3781 gggggtactg caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc
     3841 gctttttcaa tcacttgctc aatgcttaag gtggtggaat ccaacactaa agcatcggct
     3901 gccggaacca gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg
     3961 gccaaaaggc gctcaaagtt aacactaaag cccttctcct gcaactgtag catgcggcga
     4021 tgcgcacgtt cttccgagga ggcgtcaagg aaaattttca ctggcgcatc agggaatacc
     4081 accgttccca tgtcgcggcc atcggcaatc aggcctggta attcgcgaaa cgcgcgttgg
     4141 cgacgcaata atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc tgcattcgcc
     4201 acttcctgag tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg
     4261 ccattggttg acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttct
     4321 gacgcaacat caacatggtg atgtaatgcc gccaacgcca gtacgcgata aattgcaccc
     4381 gaatccagca gatgccattg caatgcttcc gccatagcct tacacaaggt gcctttccct
     4441 gcaccgcttg ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta
     4501 aaacaggcgt accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta
     4561 acgttgtgtg caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata
     4621 gcgggctggc agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg
     4681 atcacggatg tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt
     4741 gcgaccgcca tccagactag ccagtttttc aaagctgcca accagcccct gggtgctgat
     4801 gccacgtttc ttcaacagat caaaggagaa atcatctgct tcggactctt tatcacggga
     4861 gtatttagca ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga
     4921 agccacaccg ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt
     4981 catttcagcc agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc
     5041 aatttcgttg tcattcatca tgtccatcag gccactgtag acacggacac agccgttcgc
     5101 catcgcccat gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt
     5161 aatattgtta cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt
     5221 acttgcgact tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc
     5281 atcggcatcg cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt
     5341 ggtatcaata ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc
     5401 cagcagtaat ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta
     5461 tgtatcaaca acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc
     5521 cgaaaaaaaa cgccgacaat g
//
LOCUS       DAESHI010000194         5527 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.197, whole genome shotgun sequence.
ACCESSION   DAESHI010000194 DAESHI010000000
VERSION     DAESHI010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.197"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            241..582
                     /gene="raiA"
                     /locus_tag="KW011_004147"
     CDS             241..582
                     /gene="raiA"
                     /locus_tag="KW011_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBH7662335.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    685..810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            686..733
                     /gene="pheL"
                     /locus_tag="KW011_004148"
     CDS             686..733
                     /gene="pheL"
                     /locus_tag="KW011_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HBH7662336.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            833..1993
                     /gene="pheA"
                     /locus_tag="KW011_004149"
     CDS             833..1993
                     /gene="pheA"
                     /locus_tag="KW011_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HBH7662337.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="KW011_004150"
     CDS             complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="KW011_004150"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HBH7662338.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="KW011_004151"
     CDS             complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="KW011_004151"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HBH7662339.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4447..4812
                     /locus_tag="KW011_004152"
     CDS             4447..4812
                     /locus_tag="KW011_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HBH7662340.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            4962..5480
                     /locus_tag="KW011_004153"
     CDS             4962..5480
                     /locus_tag="KW011_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HBH7662341.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
BASE COUNT         1519 a         1391 c         1241 g         1376 t
ORIGIN      
        1 tgagctgccg tttttttatt ctgttagttg tgaaactgaa gcgatttagt cactatcgat
       61 ctcatcaaat atggctcgct ttgagatatt cctcaagtaa aaaaacacct cttcctgcga
      121 tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat tatgagattc ccatcacaca
      181 ttttgacatc aggaacggta tgctgaattc accaagacgg gaagacaaga ggtaaaattt
      241 atgacaatga acattaccag caaacaaatg gaaattactc cggccatccg ccaacatgtc
      301 gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc tgattaatcc acatatcatt
      361 ctgtccaaag agccacaagg gtttgttgct gacgccacaa tcaatacacc taacggcgtt
      421 ctggttgcca gtggtaaaca tgaagatatg tacactgcaa ttaacgaatt gatcaacaag
      481 ctggaacggc agctcaataa actgcagcac aaaggcgaag cacgtcgtgc cgcaacatcg
      541 gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt agtcctttat attgagtgga
      601 tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt gaaaacagta cgggtactgt
      661 actaaagtca cttaaggaaa caaacatgaa acacacaccg tttttcttcg cattcttttt
      721 taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca gaatgcgaag acgaacaata
      781 aggcctccca aaccgggggg ccttttttat tgataacaaa aaaggcaaca ctatgacatc
      841 ggaaaatcca ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa aattattagc
      901 attactggca gagcggcgcg aactggccgt cgaggtggga aaagccaaac tgctctcgca
      961 tcgcccggta cgagatattg atcgtgaacg cgatttactg gaaagattaa ttacgctcgg
     1021 taaagcgcac catctggacg cccattacat tactcgcctg ttccagctca tcattgaaga
     1081 ttccgtatta actcagcagg ctttgctcca gcaacatctc aataaaatta atccgcactc
     1141 agcacgcatc gcttttctcg gccccaaagg ttcttattcc catcttgcgg cgcgccagta
     1201 tgctgcccgt cactttgagc aattcattga aagtggctgc gccaaatttg ccgatatttt
     1261 taatcaggtg gaaaccggcc aggccgacta tgccgtcgta ccgattgaaa ataccagctc
     1321 cggtgccata aacgacgttt acgatctgct gcaacatacc agcttgtcga ttgttggcga
     1381 gatgacgtta actatcgacc attgtttgtt ggtctccggc acgactgatt tatccaccat
     1441 taatacggtc tacagccatc cgcagccatt ccagcaatgc agcaaattcc ttaatcgtta
     1501 tcctcactgg aagattgaat ataccgaaag tacgtctgcg gcaatggaaa aggttgcaca
     1561 ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct ggcggcactt tgtacggttt
     1621 gcaggtactg gagcgtattg aagcgaatca gcaacaaaac ttcacccgat ttgtggtgtt
     1681 ggcacgtaaa gccattaaca tgtctgacca ggttccggcg aaaacgacgt tgttaatggc
     1741 gaccgggcaa caagccggtg cgctggttga agcgttgctg gtgctgcgca accacaatct
     1801 gattatgacc cgtctggaat cacgcccgat tcacggtaat ccatgggaag agatgtttta
     1861 tctggatatt caggccaatc ttgaatcagc ggaaatgcaa aaagcattga aagagttagg
     1921 ggaaattacc cgttcaatga aggtattggg ctgttaccca agtgagaacg tagtgcctgt
     1981 tgatccaacc tgatgaaaag gtgccggatg atgtgaatca tccggcactg gattattact
     2041 ggcggttgtc attcgcctga cgcaataaca cgcggctttc actctgaaaa cgctgtgcgt
     2101 aatcgccgaa ccagtgctcc accttgcgga aactgtcaat aaacgcctgc ttatcgccct
     2161 gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta acgtttgatt aacgccagat
     2221 tacgctctga cgacataata atgtcggcat aaagctgcgg atcctgagca aacagtcgcc
     2281 cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag cgccagaagt tgctcaagct
     2341 gaacattttc ttctgccaga tgcagcccat aagcaaaagt agcaaagtgg cgcagagcct
     2401 gaataaacgc catattctga tcgtgctcga cagcgctaat acgatgcagc cgagcgcccc
     2461 agacctgaat ttgctccaga aaccattggt atgcttccgg cttacgtcca tcacaccaga
     2521 ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa catcgggtgt aaccccagta
     2581 ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc atttttcact gatgccagat
     2641 caaccagaat acaatctttc ggtaaaggcg gtaatttgcc gataacttgc tcagtaacgt
     2701 ggattggcac actaacaatc accattccgg catcggcaac aatatcagcc gctcgatccc
     2761 agtcatgttg ctccagaatc cgcacctgat aacccgagag tgtcagcatc ttctcgaaca
     2821 ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg acgcagtgac ggacaaagtg
     2881 ttttaaatcc tttgtcgttt tcactggagt aagattcacg catcacccga cgcaaaacat
     2941 cctcaatcag atctggcggt acacccagag cttccgcctc tgcacgacgc gaggccaaca
     3001 tagatgcctc gcgctccgga acataaatag gcagtccaaa gcggcttttc acctcgccca
     3061 cttcagcaac cagttccaga cgcttcgcta ataaattcag cagcgcttta tcgacttcat
     3121 caatttgatc gcgtaatgcg gtcaattcag caaccataat aaacctctta agccacgcga
     3181 gccgtcagct gcccgttcag atcctgatga atttcacgca gcaaggcatc ggtcatttcc
     3241 cagctaatgc aggcatcggt tacggataca ccgtatttca tttcactgcg cggttgctca
     3301 gaagactgat tgccctcgtg gatattactt tcgatcatca gaccaataat tgagcgattg
     3361 ccatctttga tttgagcaac cacggattct gccaccgcag gctgacggcg ataatcttta
     3421 ttggaattac cgtggctgca atctaccatc agagacgggc gcagtcccgc ctgttccatc
     3481 tctttttcac attgcgcaac atccgcaggg ctatagttcg gcgctttacc accgcgcagg
     3541 atgacatgcc cgtccggatt cccctgagtt tgtagcaacg caacctgccc tgcctggtta
     3601 atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag cgttaattgc tgttgccaga
     3661 ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa gcccggaggc catttcacgg
     3721 tgagtttgcg attccgttgt acgagcacca attgctgacc agctaaacag atcgcccagg
     3781 tattgcgggc tattcggatc taacgcttcc gtcgccagtg gcagtcccat attcaccagc
     3841 tcaagcagca atttacgcgc gatctgcagc ccggcttcta catcaaaaga gccatccata
     3901 tggggatcgt taattaaccc tttccagccg acagtggtac ggggtttttc aaaatagacg
     3961 cgcattacca gatagaggct atcgctgacc tctgcggcaa gggctttaaa tcgacgagca
     4021 tattccagag cagtttccgg atcatgaatg gaacaaggac cacaaactac cagcagacga
     4081 ggatcgcgcc cggcgataat atctgaaatg gttttacgcg agtcagcaat ctgggcttct
     4141 tgttgcaggc tcaatggaaa agcggccttc agttgttccg gagtcattaa aacctgttcg
     4201 tcggtaatat gtacgttatt cagcgcgtct ttttgcatga tggcgatcct gtttatgctc
     4261 gtttgcgata gttgatcctc agcgaggatg acgtaacgat aacacataaa gtaaagtttt
     4321 caatccatat ttcgtacatt tttctttaca cgaacaattt gctcgcgctt ttgatgctta
     4381 tttcgtacaa ataaatttac acctccttta aaaacaatcc gctatgcttt gaaaaaggag
     4441 aaagaaatga tgaaaaagtt tatcgccccc ttattggctt tactggttag cggatgtcag
     4501 attgatcctt atactcacgc gccaaccttg accagcaccg actggtatga tgtcggtatg
     4561 gaagatgcga tatcgggcag cgccataaaa gatgacgatg catttagcga ttcacaggcg
     4621 gatcgcggtc tataccttaa aggatatgcc gaaggacaaa agaaaacttg ccagaccgat
     4681 tttacttatg cccggggact ttccggtaaa agctttcctg ccagctgtaa caatgttgaa
     4741 agtgccagcc aactgcatga agtatggcaa aaaggggctg atgaaaatgc cagcacaata
     4801 cgattaaatt aattaaaacc ccacaaataa attaaacata agattttact cattgtgttt
     4861 tatttctcac attgatgacg atcgcatgaa aaaatgatat tttaatgagc attctgaaaa
     4921 aatatcgatg catttcgagc gaagatggtg gggatccctg aatgcgtttt tctcaccgac
     4981 tgttcttact tctgattctc ctgctgacgg gagcaccaat actcgctcag gagccttctg
     5041 acgttgcgaa aaacgtgcgt atgatggttt ccggtattgt cagttacacc cgctggcctg
     5101 cgctatcagg gccaccaaag ttatgtatct tttcatcttc acgttttagt accgccctac
     5161 aagagaatgc cgcgacatct ttgccttatc tgcctgtcat cattcacact caacaagagg
     5221 cgatgatttc aggctgcaat ggtttttatt ttggcaacga gtcaccgaca ttccagatgg
     5281 aattaaccga acaatatccg tcaaaggcgt tattattaat tgccgaacaa aataccgagt
     5341 gcattattgg tagcgccttt tgtctgatca tccacaataa tgacgtcaga tttgccgtaa
     5401 acctggatgc cttatcgcgt agcggtgtaa aggtcaaccc ggatgtctta atgctcgcac
     5461 ggaagaaaaa tgatggataa cgataattct cttaataagc gccccacgtt taaaagagca
     5521 ttacgca
//
LOCUS       DAESHI010000195         5504 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.198, whole genome shotgun sequence.
ACCESSION   DAESHI010000195 DAESHI010000000
VERSION     DAESHI010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.198"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(78..197)
                     /locus_tag="KW011_004154"
                     /pseudo
     CDS             complement(78..197)
                     /locus_tag="KW011_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
     gene            complement(302..1804)
                     /gene="dtpA"
                     /locus_tag="KW011_004155"
     CDS             complement(302..1804)
                     /gene="dtpA"
                     /locus_tag="KW011_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HBH7662342.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(2416..3051)
                     /gene="nth"
                     /locus_tag="KW011_004156"
     CDS             complement(2416..3051)
                     /gene="nth"
                     /locus_tag="KW011_004156"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBH7662343.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(3051..3746)
                     /gene="rsxE"
                     /locus_tag="KW011_004157"
     CDS             complement(3051..3746)
                     /gene="rsxE"
                     /locus_tag="KW011_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HBH7662344.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(3750..4370)
                     /gene="rsxG"
                     /locus_tag="KW011_004158"
     CDS             complement(3750..4370)
                     /gene="rsxG"
                     /locus_tag="KW011_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HBH7662345.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(4374..5432)
                     /gene="rsxD"
                     /locus_tag="KW011_004159"
     CDS             complement(4374..5432)
                     /gene="rsxD"
                     /locus_tag="KW011_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HBH7662346.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         1460 a         1396 c         1351 g         1297 t
ORIGIN      
        1 ccggaaacaa gtgagcgttt ccggattctt acacagccac tggatcggtc aactgatcct
       61 taactgatcg gcattacacc gttttcgaga cgttttttca ttaaatccac actgacgagg
      121 gtaaaatcct ttccgctctc acgcagggtg atatgggaag cgagagagca ggcacccggt
      181 ttgtagaaca atttcatcgg taactccttt tgtctgtggt ttaaagtatg gtagtgcgtt
      241 gctgaacaaa aaaaagccgc taacgaaaag ttagcggctt gtaaaagagt ttccctgaat
      301 tttacgctac agcagctttc gccgctttgt ctgcagcgtc atcctgcgtc atacggtgca
      361 gtttaggcgc ggtcagcagc atcagtactg caataatggc agtagcgaca ccaatctgca
      421 agaatacgcg accataaact tccagtgaca tcagcgggtc ggtaacgtta tccggcacag
      481 ccatcatacc cgcaacataa ccaccaatca ggtttgcacc ggcagtggtc aggaaccagc
      541 tacccataat gaagcccatc agacgctgcg gaacgagttg tgcaaccatt gccagaccca
      601 gaccagagat catcagttcc ccgatgctct gcaggccata gcttgcgacc agccagctta
      661 cagacacgat accagcgtca gacgcgaatt tcgcacccag cggcagaatc aggaacgcac
      721 cagaacacat caccatgccg attgcaaact tggttggcat cggcagggta tcgcccatct
      781 tgttatagat agcggccaga atcggactac cgatgatgat ccagaacggg ttcagtgcct
      841 gatactgctc aggttctacg gccagaccca gaatggagtg ctcaacgtta cgaatcgcaa
      901 agaagttcag tgacgttggc atctggctgt acagcacgaa gaagataatg gcttcgagca
      961 tcaggatgaa ggcaacgatc attttacgac gcgcagcacc tttcatcgcg aaggcttctt
     1021 taccgaagat aaccacgata ccgaaggcaa caacgcccag cgccatacgc gcaacttcct
     1081 gattgtgcag cagccaggtg gcgatagcga tcagtgccac aacaccaata atggtcagca
     1141 gcaggttacg gtagttgata ggctcgaagt ctggttttga accgtactgt ttaacccagc
     1201 gttggcagaa ggcgaagtta acgatagtga tcagcaggcc tacaacgctc aacgcaaacg
     1261 caacactcca gccgtatttc gcggccagcc acggcgtagc aatcatagag aagaaagagc
     1321 cgatgttgac ggacatgtag tacatggtga atgcaccgtc cagacgcggg tcgtttttct
     1381 catagcatgt agaaagcaga gaagatgggt tagctttaaa caggccgtta ccgaccgcaa
     1441 tagccgccat acccatataa acgataccgg cgtcgtgacc agaccaggca accagagcat
     1501 aaccaatcgc cagcacaata gcgccgagca taattacgcg tttagtaccc agtaccttgt
     1561 cacctaacca gccgccgata gcgaccagac cataaaccag ggcactaaag gaagagaaaa
     1621 gggtgattga atccgcttca gacataccca gttgtttaac caggtaaaca gccataattc
     1681 cttgtaggcc gtaataacca aaacgttccc ataactcaat cgagaagatg agatagaacg
     1741 ccttcggttg tttgaaagcg ttcaaactga cgctttcagt tggtttttgg tttgcagtgg
     1801 acacttttac ctcttttttt acatcccata ttaacggggg ggctttctcg cgttatgtct
     1861 aaaaaacata cgcgcaattg ttatagtggg aggggaaacg gcaggtaatg ttcactatct
     1921 tgcactttca gacaagtcct ttgtaataat ctgttacata taactgaggc ggggaattca
     1981 accattccgg gaaatgttat ccagcgttaa atttttcaaa aaacgtaatg gcagattttt
     2041 tcactgcact gagagggggg gtgatggctt aacggcgata tgttctgcta tttggcgttt
     2101 ttactagtga gatagtccag tttctgaaaa atagccagtg taatgttttg taggtcaata
     2161 tttgttgcgt gtgtaaattt aaattatttt aacctgattc ttgcaatgtt tttacattgc
     2221 gacagcatcg tgatcttgat cacgaatgaa tagaaaattt cgctattaaa aaaacaatta
     2281 acctgcattt ttggttatcc gcaaacggat tacctgatga taacgctaag caatggcatc
     2341 attcacgtta aaaagatgtt ttaaaagagg ataaagaaag gttatcaatg gggtaatcgg
     2401 tgttacccct tttcttcaga tgtcaacttt ctctttgtat tcacaaagat cttcaataat
     2461 acaagagcca cagcggggct tgcgggcaat gcaggtataa cgcccgtgca ggatcaacca
     2521 atggtggcag tcgactttaa actctgccgg aaccactttc agtagctttt cttctacctg
     2581 ttcgacgttt ttccccggcg caaattgagt acgattacaa acgcggaaaa tgtgcgtgtc
     2641 gacagcaata gtcggccagc cgaatgcagt gtttaatacg acgttggctg ttttacgacc
     2701 tacgccgggc agggcttcaa gcgcagcacg atcttccgga acctcgccat tatgctgctc
     2761 cagtaagata cggcaggttt tgatgatatt ttctgctttg ctgttataaa gcccaatcgt
     2821 tttgatatag gttttaaccc cttcaacgcc cagttcaagc atcgctgcag gcgtattcgc
     2881 caccgggtaa agtttcgccg tcgccttatt aacactgaca tcggtcgcct gagcggaaag
     2941 cagtacggca atcagcaatt caaaaggcga actgaaatta agctcggtgg tgggatgagg
     3001 attgttctca cgcaggcgag tgaggatctc caggcgtttt gctttgttca tcagacattc
     3061 cctgtttcac cgtttggcaa tgcacgttcg gcagctgctt cagtacggcg ctttttcatt
     3121 ctttcatcaa tcaggtattt tcctgccagc atcagtccca ggccaataaa tgcacctggt
     3181 ggcagcatcg ccagcaggaa aggggagtcg gtgtggaaaa tctccacgcg taatactttt
     3241 gcccagctac ctaacagcgc atctgcaccg tcaaacaatg tgccattgcc gataatttcg
     3301 cgtagtgaac ccagcacgaa catggcgcag gttgcgccca taccaattga aaagccgtcc
     3361 agtgccgaaa gcgccggacc ttttttggcg gcgaaggctt cagcgcggcc cacaacgata
     3421 cagttagtga caatcagcgg aataaaaatc cctaatgatt gatacaggcc aaaggcgtag
     3481 gcgttgatca gcatctgtac agcgctgacc accgaggcga tgatcatcac gtaaatggga
     3541 atgcggatct cggctggcgt ccagtgacgc agcgtcgaaa tggtcaggtt ggtcagcgtc
     3601 agtaccagcg tagtcgcaag tcctaaaccc agagcgttag tggcagtgga cgtgaccgcc
     3661 aacagaggac aaaggccgag caactggacc agcgcagagt tgtttttcca caacccctga
     3721 acaataacgt ctttaatttc gctcacgctt tattctccac aggcaggaag ttgagaaagt
     3781 tgtgccggta acgtctgagc gtacaatccg gcgcgtttta ccgcattaac caccgcgcgg
     3841 ggagtaatcg tcgcgccggt gaactggtcg aaatcaccac catctttctt caccgcccag
     3901 tgcgcatcat ccgcaccact gattttttta cccgcaaaat gggtgatcca gtcagaaagg
     3961 cgcagttcga ttttatcgcc aagccctggc gtttcgtggt gctctgtcac gcgcgtgcca
     4021 agtaccgtgc cgttaaaatc ggctcccacc agcagctgaa tcgcaccgga atagccatct
     4081 ggcgcggttg cttccagaac ggcggctacc ggtttgtcat cctgtttggc gatgtaaacc
     4141 cgatgctcac ctttacctaa ctctggcgca gttaccagat agcaactctg tgccagcgta
     4201 ttgttatagc gttcggctgg cagcacctga tcaaataatg ccttttgttg cagactggcc
     4261 tgttcagcaa tcgtcgtttt ggtcatctgg ttgatggccg cagttaaccc tgttgaaccc
     4321 gctgcaaata gcgctaacgt aatgccgtgt tttcggatag ttttcagcat ggtttaccct
     4381 ttgcgatggc cgtagacgcg cggacgcgtg tagtaatcga tcagaggaac cgtgatgttc
     4441 gccagcagga cggcaaaagc cacgccgtca ggatagccgc cgaaactgcg gatcaaccag
     4501 actaataagc ccgcaagcgc gccgaaaata agacgaccac gattggtcgt agaagcggta
     4561 accgggtcag tcaaaataaa gaatgcgccg agcatggtcg caccagacag cagatgaatt
     4621 tgcggtgctg ccagtgtttc tggtgagaac aaccagccca acgttgcgca taacgccagc
     4681 gttactaaga agctgagggg aatatgccag cgaatcgctt tctgccatag caaccacagg
     4741 ccgccagcca gccaggcgag atttacccat tgccaaccag cgcccgccag aataccgctg
     4801 tagatcggat attgcataat ctgttcaacc gaatgaccgg cacggacaga ggttttaaat
     4861 gtatccagcg gtgtcgcctg actaatgcca tcaataccta agcgtagtgt gttcatatca
     4921 ccaccactgg cggtatgccc gctgaaaata acctgtatgg cgtcgataaa accagggatg
     4981 ttgaccgcaa tttcatgtgg cggtaaccag ctggtcatct gcaccgggaa ggagatcagt
     5041 aagaccacat aaccaatcat tgccggatta aacgggtttt gccccagacc gccatacaac
     5101 tgtttagcga tgatcaccgc aaacaccgta cccagcacga ccatccacca tggcgcgagg
     5161 gggggaatac ttaccgccag caataagcct gtcagcaatg ctgagttatc tttcaacgtt
     5221 gcggctaccg actgcttgcg tagtttgagt acgagagctt cggctaacag agtactaacc
     5281 gatgccaaca ggatctgaac gagagtaccc caaccaaaaa accacagttg cgctgcgatt
     5341 cctggcacgg ctgcgagcaa caccaacagc ataatgcgcg atgtctggcg ctggttatgg
     5401 gtataagggg agctagctat tctgaatacc atttagtcct cgtttacaac cttctgctgg
     5461 gcggcttttt tggcctgagc gcgggcaata gccgcggcaa ctgc
//
LOCUS       DAESHI010000196         5235 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.199, whole genome shotgun sequence.
ACCESSION   DAESHI010000196 DAESHI010000000
VERSION     DAESHI010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.199"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..120
                     /locus_tag="KW011_004160"
     CDS             <1..120
                     /locus_tag="KW011_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH7662347.1"
                     /translation="RPVKASSEAVQMLISQGKTRLQIQEELGISRATYYRLAK"
     gene            489..1151
                     /locus_tag="KW011_004161"
     CDS             489..1151
                     /locus_tag="KW011_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBH7662348.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPFLLFKNLHREFIFSLDGAATEHD
                     TLLPRFTDDISRQSWVSEKVFCNPPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNRLTIRSPFPSAVVVFKKEPRKHEITQMVCCAD
                     TLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKYIADALRMPLSTSYRIAQR
                     LS"
     gene            1515..2401
                     /locus_tag="KW011_004162"
                     /pseudo
     CDS             1515..2401
                     /locus_tag="KW011_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049841.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            2401..2690
                     /locus_tag="KW011_004163"
                     /pseudo
     CDS             2401..2690
                     /locus_tag="KW011_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3011..3301
                     /locus_tag="KW011_004164"
     CDS             3011..3301
                     /locus_tag="KW011_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662349.1"
                     /translation="MRHTVTRLLPEAYSRLAAIKDKYPAMSHTALVGEILKEHTALTP
                     FDAIKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
     gene            3301..3720
                     /locus_tag="KW011_004165"
     CDS             3301..3720
                     /locus_tag="KW011_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662350.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYVKSLINKDN"
     gene            3888..4211
                     /locus_tag="KW011_004166"
     CDS             3888..4211
                     /locus_tag="KW011_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662351.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNAENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
     gene            4641..>5235
                     /locus_tag="KW011_004167"
     CDS             4641..>5235
                     /locus_tag="KW011_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662352.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYACFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKL"
BASE COUNT         1726 a          947 c         1074 g         1488 t
ORIGIN      
        1 agacctgtaa aagcttcttc agaggctgta cagatgctta tttcacaagg taaaacgagg
       61 ttgcagattc aggaagagct aggaatctct agagcaactt attacagact tgctaaataa
      121 gttactgaat agttacatgg atgtagctga tatttggctt ttttgccaag ggggtcacat
      181 agctggtgtg acgggtacta aacacgtata catcatgagg tagattctac cgaatcaact
      241 agacagatat tttcgaagat tcggggaaga tgagattcaa agagggctag tcatttttta
      301 taaaaatttt cagactctga aaagttactg tacagaatat atacagatat ctttaaagta
      361 tctgtaaaaa cttttaagat aaaagataga tatcccagaa aggatatccg tttagaaaac
      421 ctgttaagta tctcttaaaa gagagtaaac cttgtcaaga gtgattgcaa taaaaatttt
      481 tatttcttat gtcaattgag catattttaa tcgggaagca tcatgggggt tctgattact
      541 ggagaactcc atttttgtta tttaagaact tacaccgtga atttattttc agtctagatg
      601 gtgctgcaac tgagcatgac acgctgttac cacgattcac cgatgatata agccgtcaat
      661 catgggttag tgaaaaggtc ttctgtaacc cgccgtacag cgatattccg tcgttcctct
      721 taaaagcgtc tgaagcggat ttagtagctt tcctgatacc gcatagagcc aacaccagct
      781 actggcttag gcacatttac agcaataacc actgtcatga aatccgtatt cttcatcgtg
      841 cagttaaata ccttccacca gcagggcata acaggcttac tattaggtca ccgtttccaa
      901 gtgcagtcgt tgtttttaaa aaagaaccac gtaagcatga aattacacag atggtttgct
      961 gtgctgatac attgctaccg ttaaccatca ttaaccgtgg tggactaaga ggaagaccca
     1021 ccatttaccc acctgagacg ctcgacagct tcataaaact atacaggcaa ggaaaaccca
     1081 ttaagtacat tgcagatgct ctcagaatgc ctctgagcac gtcatacagg attgctcaaa
     1141 ggttgagcta aaattctcat aaatatggtc atttacaaga caggtgattc agatgttaag
     1201 aagtatccct gtgaacaata aatgaccaat cagtaatcat tctgtgaaat aattgttgca
     1261 atttataatc acatctgtta tagtttctat atgggctgat aaaaaagttc atgtcttgaa
     1321 gtggtaacac ttggggaggt gtcagatggt tggcgtatat taacctctca ttcagggtat
     1381 acctcagcgt tttaatcatc gtactgtttc aggcatggac ttctttatca gtccatcaat
     1441 cccccagata ttttaatcac acaggctaat cggttttatc cggttggcct tttcacgtat
     1501 gaggtaattt atgaatgtct aaatataacc caaatcagtt ttataaattt gatgtgcgaa
     1561 tctctcaact ttcagaagaa attaacgcag cagcaagatt gatttatatg gtcatggtcc
     1621 atagctgtga cttctatgaa gagaaataca aatcatattc accaagtatt gaccatctcg
     1681 cttatatggc tggtgtatca aagagaacag caatcagagc cattgagaca cttgagaaac
     1741 acggtttaat tcaggtgcaa aagaatcagg ggcgtcaaag cacttatcgt gtattcacac
     1801 tggatgataa gccggaatta cttgtaagac ctacatcaca ggaagttgca gcctcagtaa
     1861 gacaaaatga caccactacc agtgacaaaa tgtcatgggg gtagtgacaa aatgtcaccc
     1921 gtgttagaga gataagttat tagataaaac attagataat aatattacag ataagaataa
     1981 agagactgga cttaatgatg atattaaaga agatgacttt ctttctttgg atgaattaaa
     2041 tgattttctc tctatgaatg aatctgacct tgaagtaagc tctaaaaaga cttctaaaca
     2101 agacagagaa atcactgatg tgagtgttga tagtgaccat aaaaacacac agtgccccgc
     2161 cactgatgat gagttggacc ccttcgatga tgaagaggat gaacgtgaaa aagccattaa
     2221 gagggcagag aagaaaaaga gattcgagct tgcaagacaa aagaacaaag tatcgggttt
     2281 agacccattc gatgacagtg aagatgatta ttatctttca ccgaaaaaga aacaaactca
     2341 ggatgacaag aagcaaaaaa ttaaaccgaa gatgtatttt gccggtggag gaattaagta
     2401 atgaaagaat atcacggtga aaagagatac aaagattacc tgttaaagcg ttacagcatt
     2461 tctcgtgaag gtcatcttat gaaggatact cacgggaagt ttaccggatt agaccaaaga
     2521 aagaagggag gggttatttc ttttacgatg gtgtgacaga tttaaagatt gacgctttga
     2581 ggtttgcagt gctgtatcat tttgatatct ggaattcaat ccaccagcta cgattaaaag
     2641 acggtgaccc cagcaactta aaggccacca atattatcac aaaacgttag tttgttttac
     2701 catcatcaat cgtaattgtg atttcatatt tgaaaagctt aatgctgaaa ttgaatcgtg
     2761 tacttctggc gataatcatt tttaaaagct gatacacaag aagcaccaga aacacaaagc
     2821 aacctaagct tgcgaatctg tgagattcag caattgtttc aagaaagccc attaattccc
     2881 cttatttatc aaagggaatg acctgtatca gccccctaca accatattaa catgtaaatg
     2941 agttgttgac aatatgttat aaatatgtta aagttagtta taagtcaaag attttaagaa
     3001 ggaaaattta atgagacaca cagttacacg ccttttgcca gaggcatatt ccagactggc
     3061 agcaatcaaa gacaaatacc cagccatgag ccacacagca cttgttggtg agattctgaa
     3121 ggaacacaca gcccttacac cgtttgacgc tatcaagggc gttgcagagg gttctaaggc
     3181 catcagaatc acaacgggcc aactggaact actcaaagca cagtccgcag tggcaggttg
     3241 ctcaatgatt caggtgttga gtgccaaggt tcatcaactg gcggataagc tgggggttta
     3301 atgctatgca catttgatta tcatgcgttt tatcgccata gaggtcacct tgtccgtgag
     3361 aataacggaa catggaaaga tttaactcgt aatgctgaac ccttcacaca ccagcaatta
     3421 aaatcccgct tctccagatg gaagaatgca ccagcagata ctggtgattt tgagattatc
     3481 cataccttcg attatgcctc agaagatgac cttcacgatg caattaagct gattgaagac
     3541 ttcaatacct cagaagatgt ggatggtgac aaatataaat cccgtggtaa ccgctataac
     3601 cgtgatgata tcactcaggc cataaatgct ggtcaatctc agcgatacat tacagaaaaa
     3661 tatggtattt cacggagaca gtatttctac gttaagtcat tgattaataa agacaattaa
     3721 aaataaccat ctaagaaacg gtagaatgca ctttacaggc agagttgcac cttaatttga
     3781 taaattacct taccagccag tgcaactccc tctgtaaccc gcatggttac tggcctcaga
     3841 atggctattt taagaggtct gaggtgtgtt tcataggaga tttaaaaatg aataattacc
     3901 caatgcaaat tatggttgat aacgacactg ctatgatggt gcaaagcttc attgatgcag
     3961 gtatcgcaat taattttgat aaattactaa agctgatggc tgataatgca gaaaacatct
     4021 ctgactttat tcagtcggtg gagtttaacg agcctcgaat gatgctacca attacagaca
     4081 gcaacatgaa aagattggtt atcgagcaaa cgaacatata cagtatttcc ccagagcaat
     4141 ttttgaaagg tgctgtgaca atcctgtaca gtgataatat tctggtgacg gattctgtga
     4201 gggttcacta atgggtttaa taattgcttg gacgttgctt ggcttatttg ttggttgcat
     4261 tattaacaat gcggtaaata agaattgatg ttatcaacct gttaaaaagc tgttgacaaa
     4321 taaccttaag catggcataa ttttaattat gggagagcaa ttctctctta tttttctttc
     4381 aataatccga tggatggata ttctgagttg gaaaggtgca atgcttttcg tcatggcctt
     4441 tcaggttagt ctgagaggct atctcaaaaa gtactcttgt tcattcatta ttgaatcctt
     4501 tgcccttcac agaaatgttt agggcttttt cagtttttaa aagcacactt ggcaggttct
     4561 tgttcagtca gactcttcac ctctgattgc tgagtgtgct tctataaatt gaaataacaa
     4621 ataaactaag gggtgaacga atgattagaa atgaacatga aatgcttatc gaacacagac
     4681 caagtgcact tgatattcac gaattaactt tgcgtgaatc ttttgataga gcttggcggg
     4741 aatcacacga agtaaataaa ggtccatata ttccaccaga accactcgag attccaagag
     4801 ttgaaattga tttttctatg aacgaacggt gtgaacttga tttgaagttg aagctacaac
     4861 gccgatattt ccaagctatt aaagactctc aagaagcaat gagaagtgtt tacgagaaaa
     4921 ttcagtccaa gcttgaagac gaaattgacg aaatggttat ttcgatggag cttcaaacca
     4981 attcttatgc ttgtttctct cgactagacc gtgacggttg ggggtatgac cctgtagaga
     5041 ttggtaaagt gattgcagat ttaccagaag gtcaaagagt tgttcgtaca gaaagagctg
     5101 tcagagcacc acatcgactg tctctaattg ttgctgataa atcacaagct gagcttgaag
     5161 aaattgccaa agagaaactg atgaaagcaa gagaaaatga gattaatttg ctgaaatcaa
     5221 gactggctaa gctga
//
LOCUS       DAESHI010000197         4844 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.200, whole genome shotgun sequence.
ACCESSION   DAESHI010000197 DAESHI010000000
VERSION     DAESHI010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.200"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(34..954)
                     /locus_tag="KW011_004168"
     CDS             complement(34..954)
                     /locus_tag="KW011_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH7662353.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1092..1991
                     /gene="pgrR"
                     /locus_tag="KW011_004169"
     CDS             1092..1991
                     /gene="pgrR"
                     /locus_tag="KW011_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HBH7662354.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            2328..3941
                     /gene="mppA"
                     /locus_tag="KW011_004170"
     CDS             2328..3941
                     /gene="mppA"
                     /locus_tag="KW011_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HBH7662355.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(3992..>4844)
                     /gene="ynaI"
                     /locus_tag="KW011_004171"
     CDS             complement(3992..>4844)
                     /gene="ynaI"
                     /locus_tag="KW011_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HBH7662356.1"
                     /translation="INYVIENYKLKFITPDVIDFICTSLIAVILTIKLFLLINQFEKQ
                     QIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKD
                     ILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSL
                     FSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYF
                     NQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNI
                     TPPEQGR"
BASE COUNT         1307 a         1081 c         1140 g         1316 t
ORIGIN      
        1 ccatatcact attgcggatt tgaatatgtt tcattacaga gtttcctcaa agaacggagc
       61 taatactgaa attgcttcag ccacataggc tttgccgtca taaagatcca tatggtttgc
      121 gccttcaaca atgtgatagc gtttatcttg gcttgatgct cgatcgtaca ggtcgtcact
      181 catccatttg ctccctgcct ggctgcccgc cacaatctgc atcggctgag tcaggtacac
      241 ttccgccata tggtaagcat caaaggtaat aatctggtta aggctgcgca aagtagcgta
      301 acccggtgct gttggatact gcgcgcgagg ggtgtggtaa tactcccagg cctgacgcag
      361 ttcttcgttc ggcgcatcgg actccttcat tggtgccagt ggcataacgg catattctcc
      421 gctgctaata tcgctggttc gggcgtttga acccgcttca acgtatggca gggcatcaat
      481 agatttcaca ttgttttccc aaccattacg gaacatcgaa ccaatattga ccgcactaac
      541 ggtaccgatg gccttgatgc ggcgatcctg aattgcagca ttggctgtat atcctgcacc
      601 ggcacaaatt cccatcgcac caattcgggt attgtcgaca taagaaagcg ttgtcaggta
      661 atcaatcacg gcactgatgt cttcagtacg aatgtatggg ttttctaact gacgcggctc
      721 gccgccactt tcaccttgat aagatgcgtc ataagcaata gtgacaaatc ccttttccgc
      781 cagttttttg gcataggttc cggccgtttg ttctttaacg cccccacctg ggtgagatac
      841 cacaattgcc tgatactgac gggtttcatc aaattttgga gggaaataga tcactgcaga
      901 caaagagatg gttggattat tgctgttagt gaagctgact ttattattca tcattccgtt
      961 cctcatggag ttgtcggttc gttttaacgg ttggtgatat cactatagat attgatcatt
     1021 aagttgatta gaccctaaat tatgattaga cctatcattt aaatgattaa tagattgcat
     1081 ggagatactg aatgaagcgt gaagaaatcg ctgatctgat ggcgtttgtc gtcgttgcag
     1141 aggagcgtag cttcactcgt gcagcagccc gcctgagcat ggcgcagtca gctttaagcc
     1201 agatagtgcg tcgtatagaa gaacgattgg gattgcggct tctgacgcga accacgcgca
     1261 gcgttgttcc aactgaagcg ggcgagcatc ttttgtctgt tcttggccct atgttgcatg
     1321 acatagattc agccctggca tccctgagcg agctgcagaa ccgcccatcc gggacaatac
     1381 gtattactac tgtagaacat gcagccaaaa cgatattgtt accagcaatg cgcacattcc
     1441 tgaaatcgca tcctgaaatt gatattcagc tcaccattga ttatggtttg accgatgtcg
     1501 tttctgaacg ttttgatgca ggcgtccgtc tgggtgggga gatggataaa gatatgatcg
     1561 ccattcgaat cgggccagat ataccaatgg ctattgttgg ctcaccggat tatttttctc
     1621 gccgaagtgt tccaacgtca gtgtcacaat taatagatca tcaggcaatt aatttgtatc
     1681 ttcccacatc gggtacagca aatcgctgga gattaatacg cggtggacgt gaagttcgtg
     1741 ttcgcatgga aggtcagctt ttactgaata cgatagacct gatcattgat gctgcaattg
     1801 atgggcatgg attggcgtat ctaccttatg atcaggttga gcgggctatt aaagaaaaaa
     1861 aactgatacg tgttttggat aaattcacac cagatttacc cggttatcac ctgtactatc
     1921 cacaccgtcg acatgctggc tcggcattct cattatttat agataggctg aagtataaag
     1981 gtgctgttta gcactacttg ctgatacatt aatttaattt ttctcttaac gtattctcag
     2041 ttcctttcaa cgttttggtt attttttatt cttcgtacaa tggcgacaga tgctgattat
     2101 gataccgaaa actggtttga acgtgcgaag cctgaacgta gtgttcggtg ttctatgtgc
     2161 tttaccgcat tttgtcgtct attatttaca ctaaatctga tttaatatat tgatacataa
     2221 aacattttat gctccctgtg tcactttttt gatggaagtt gtttgcattt ctttaaggcg
     2281 aaacaaataa ttacgcatca attttaatgt cggttagagg gaaacttatg aagcactctg
     2341 tttcagtcac gtgttgtgcg ctgttggtca gcagcatttc tctttcgtat gctgcagaag
     2401 ttccgagcgg cacagtactg gcagagaagc aggagctggt gcgccacatt aaagatgagc
     2461 ctgcgtcgct ggatcccgct aaagccgtgg gcctgccaga gattcaggtc attcgcgatc
     2521 tgtttgaagg actggtgaat cagaacgaga aaggggagat tgtccccggc gttgcgactc
     2581 agtggaaaag taatgacaac cgtatctgga cttttaccct gcgcgataac gcaaaatggg
     2641 cggatggcac accggtaacg gcgcaagatt ttgtctacag ctggcagcgt ctggtggacc
     2701 cgaaaacgct atcgccattt gcatggtttg ccgcgctggc gggaatcaac aacgcacagg
     2761 cgattattga tggtaaagct acgccagacc agcttggcgt caccgcagtt gatgcccata
     2821 ctttgaaaat tcagcttgat aaaccgttgc cgtggtttgt gaatttaacc gctaactttg
     2881 ccttcttccc ggtgcaaaaa gccaacgtag aaagcggtaa agagtggacg aaacccggaa
     2941 atctgatcgg caatggcgct tatgttctta aagatcgcgt agtcaatgaa aaactggtcg
     3001 tggtaccgaa tacccattat tgggataacg ccaaaacggt actgcaaaaa gtgaccttcc
     3061 tgccaattaa tcaggaatcc gcagccacta agcgttacct cgcgggggat attgatatca
     3121 ccgaatcctt cccgaaaaat atgtatcaga agctgttgaa ggatattccg gggcaggttt
     3181 atacgccgcc gcagctcggg acctattatt atgcgtttaa cacgcaaaaa gggccgacgg
     3241 cagatcagcg cgttcgtctg gcattaagta tgacgataga tcgccgcctg atgaccgaaa
     3301 aagtattagg gacgggcgaa aagccagcgt ggcattttac accagatgtt accgccggat
     3361 ttacgccgga accttcgccg tttgaacaaa tgagtcagga agaactgaat gcgcaggcaa
     3421 aaactttgtt gagcgcagct ggttatggtc cgcaaaaacc gctgaagctg acgcttttgt
     3481 ataacacttc agaaaaccat caaaaaattg cgattgctgt agcatcgatg tggaaaaaga
     3541 accttggcgt agatgttaaa ttgcaaaatc aggaatggaa aacctatatc gatagccgta
     3601 acaccggcaa ttttgatgtt atccgcgcct catgggtggg ggattataat gaaccctcca
     3661 ctttcctgac attattaacg tcaacgcatt caggaaatat ttcacgcttt aacaatccgg
     3721 catacgacaa agttctggcc caggcatcga cggaaaatac cgttaaagcg cgtaatgccg
     3781 attacaacgc ggcagaaaaa atcctcatgg agcaagcacc gattgcacca atttatcaat
     3841 ataccaatgg acgattaatc aagccgtggc tgaaaggtta tcccattaat aacccggaag
     3901 atgtggcgta cagccggact atgtatattg tgaagcattg atgtgatggg aactggcgtt
     3961 acccttgtgg ataacgccag tgatgtctga tttagcgccc ctgttccggc ggtgtaatgt
     4021 tatccatata cagcgtctgg ctggggaagg caaagtccgc gccgtgtgac tgcacaatat
     4081 cgataatctt caaataaacg tcttgctgtg cagcaagcca ttcagcccat accgtggttt
     4141 tggtaaagca ataaaccata atattcaatg acgagtcggc aaactggttg aaataaacca
     4201 gtaaggtttg tcgctggtcg atggccgggt gatttttcag catctcacgt acggcttcga
     4261 caataacgcc cacttttgcc gcatcctcat aacgtaaacc aatggtcgtg gtaatgcggc
     4321 ggttggtcat tcgtcctggg ttttctacgc tgatcgacga aaacagcgag ttcggtacgt
     4381 acaatggacg attatcaaag gtcgtaattt tggtaattcg ccagccaatt tccgctactg
     4441 taccttcgat atttctgtcc ggtgaacgga tccagtcgcc aatactgaaa ggacggtcga
     4501 aatagagcat gatcccggaa aagaaattac tcagaatatc tttaccggcc ataccgacag
     4561 ccagaccacc aataccacca aaggtcagca agccagaaag gctcatgccg aaatgttcgc
     4621 cataaagcag aacaagcacc acaataatgg tgattttgat aatgcgcgac ataatccgcg
     4681 cactggtgat atcgcgacct tttttaatct gctgtttttc aaactgatta atcagcaaaa
     4741 atagcttaat cgtcagaata accgcaatca gggacgtaca gataaaatcg ataacgtctg
     4801 gggtgataaa tttgagttta tagttttcta taacataatt aata
//
LOCUS       DAESHI010000198         4832 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.201, whole genome shotgun sequence.
ACCESSION   DAESHI010000198 DAESHI010000000
VERSION     DAESHI010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.201"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(108..551)
                     /locus_tag="KW011_004172"
     CDS             complement(108..551)
                     /locus_tag="KW011_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBH7662357.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            708..1637
                     /gene="metA"
                     /locus_tag="KW011_004173"
     CDS             708..1637
                     /gene="metA"
                     /locus_tag="KW011_004173"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBH7662358.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            1906..3507
                     /gene="aceB"
                     /locus_tag="KW011_004174"
                     /pseudo
     CDS             1906..3507
                     /gene="aceB"
                     /locus_tag="KW011_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            3537..4828
                     /gene="aceA"
                     /locus_tag="KW011_004175"
                     /pseudo
     CDS             3537..4828
                     /gene="aceA"
                     /locus_tag="KW011_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT         1205 a         1269 c         1225 g         1133 t
ORIGIN      
        1 gcagaaggcc atcctgacgg atggcctttt tgcattggcg cagaaaaaaa tgccgggtgc
       61 gacgctggcg cgtcttctcc agcctgggca ggcacggcag gggcagctta ttcctccaca
      121 tacgccagat tcagcaacgg atacggtttc cccaaatcgt ccacctcaga gcgtcccgta
      181 accttaaaac ccaccttctt atagaaccca accgcctgct cattttgctc attaacgttg
      241 gttgtcagtt ccggtgccat cgagagcgca tgctccacca gcatccgacc cacgccgcag
      301 ccgcacacat caggatcgat aaacagcgca tccatatgct gcccacttaa caacataaac
      361 ccaaccggct gatcccgttc attaaccgcg acccacaacg gcgcttccgg caggaaggaa
      421 cgaactagca cctccagctc ggcccgatac tctgctgata aaaaatcgtg tgtggcatcg
      481 acagaacgac accaaatcgc aacgagttcc tccccttcct catgccgtga gcggcgaata
      541 ctaataacca ttttctctcc ttttcgtcat tcttatattc taacgtagcc ttttccttga
      601 aactttctca ccttcaacat gcaggctcga cattggcaaa ttttctggtt atcttcagct
      661 atctggatgt ctaaacgtat aagcgtatgt agtgaggtaa tcaggttatg ccgattcgtg
      721 tgccggacga gctacccgcc gtcaatttct tgcgtgaaga aaacgtcttt gtgatgacaa
      781 cttctcgtgc gtctggtcag gaaattcgtc cacttaaggt tctgatcctt aacctgatgc
      841 cgaagaagat tgaaactgaa aatcagtttc tgcgcctgct ttcaaactca cctttgcagg
      901 tcgatattca gctgttgcgc atcgattccc gtgaatcgcg caacacgccc gcagagcatc
      961 tgaacaactt ctactgtaac tttgaagata ttcaggaaca aaactttgac ggtctgatag
     1021 ttaccggcgc gccgctgggc ctagtggagt ttaatgatgt cgcttactgg ccgcagatca
     1081 aacaggtgct ggagtggtcg aaagatcacg tcacctcgac gctgtttgtc tgctgggcgg
     1141 tacaggccgc gctcaatatc ctctacggca ttcctaagca aactcgcacc gacaaactct
     1201 ctggcgttta cgagcatcat attctccatc ctcatgcgct tctgacgcgt ggctttgatg
     1261 attcattcct ggcaccgcat tcgcgctatg ctgactttcc ggcagcgttg attcgtgatt
     1321 acaccgatct ggaaattctg gcagagacgg aagaagggga tgcatatctg tttgccagta
     1381 aagataagcg cattgccttt gtgacgggcc atcccgaata tgatgcgcaa acgctggcgc
     1441 aggaatattt ccgcgatgtg gaagccggac tagacccgga tgtaccgtat aactatttcc
     1501 cgcacaatga tccgcaaaac aaaccgcgcg cgagctggcg tagtcacggt aatttgctgt
     1561 ttaccaactg gctcaactat tacgtctacc agatcacgcc atacgatcta cggcacatga
     1621 atccaacgct ggattaatct tctgtgatag tcgatcgtta agcgattcag caccttacct
     1681 caggcacctt cgggtgcctt ttttatttcc gaaacgcacc tcagcaagtg aataaatttt
     1741 attcatattg ttatcaataa gttatcaagt atttttaatt aaaatggaaa ttgtttttga
     1801 ttttgcattt taaatgagta gtcttagttg tgctgaacga aaagcgcaca acgatccttc
     1861 gttcacagtg gggaagtttt cggatccatg acgaggagct gcacgatgac tgaacaggca
     1921 acaacaaccg atgaactggc tttcacaagg ccgtatggcg agcaggagaa gcaaattctt
     1981 actgccgaag cggtagaatt tctgactgag ctggtgacgc attttacgcc acaacgcaat
     2041 aaacttctgg cagcgcgcat tcagcagcag caagatattg ataacggaac gttgcctgat
     2101 tttatttcgg aaacagcttc cattcgcgat gctgattgga aaattcgcgg gattcctgcg
     2161 gacttagaag accgccgcgt agagataact ggcccggtag agcgcaagat ggtgatcaac
     2221 gcgctcaacg ccaatgtgaa agtctttatg gccgatttcg aagattcact ggccccggac
     2281 tggaacaaag tgatcgacgg gcaaattaac ctgcgtgatg cagttaacgg caccatcagc
     2341 tataccaatg aagcaggcaa aatttatcag ctcaagccca atccagcggt tttgatttgt
     2401 cgggtacgcg gtctacactt gccggaaaaa catgtcacct ggcgtggtga ggcaatcccc
     2461 ggtagcctgt ttgattttgc gctctatttc ttccacaact atcaggcact gttggcaaag
     2521 ggcagcggtc cctatttcta tctgccgaaa acccagtcct ggcaggaagc ggcctggtgg
     2581 agcgaagtct tcagctatgc agaagatcgc tttaatctgc cgcgcggcgc catcaaggcg
     2641 acgttgctga ttgaaacgct gcccgccgtg ttccagatgg acgaaatcct tcacgcgctg
     2701 cgcgaccata ttgttggtct gaactgcggt cgctgggatt acatcttcag ctatatcaaa
     2761 acgttgaaaa actatcccga tcgtgtcctg ccagacagac aggccgtgac gatggataaa
     2821 ccattcctga atgcttactc acgcctgctg attaaaacct gccataaacg cggtgctttt
     2881 gcgatgggcg gcatggcagc gtttattccg agcaaagatg aagagcgtaa taaccaggtg
     2941 ctcaacaaag taaaagcgga taaagcgctg gaagtcaata acggtcacga tggcacatgg
     3001 atcgctcacc caggccttgc ggacacggca atggcggtat tcaacgacat tctcggctcc
     3061 cgtaaaaatc agcttgaagt attgcgcgaa caagacgcgc cgattactgc cgatcagctg
     3121 ctggcacctt gtgacggtga acgcaccgaa gaaggtatgc gcgccaacat tcgcgtggct
     3181 gtgcagtaca tcgaagcgtg gatctccggc aacggctgcg tgccgattta tggcctgatg
     3241 gaagatgcgg cgacggctga aatttcccgt acctcgatct ggcagtggat tcatcatcaa
     3301 aaaacgttga gcaatggcaa accggtgacc aaagccttgt tccgctagat gctgggcgaa
     3361 gagatgaaag tcattgccag cgaactgggc gaagaacgtt tctcccaggg ccgttttgac
     3421 gatgccgcac gcttgatgga acagatcacc acttccgatg agttaattga tttcctgacc
     3481 ctgccaggct accgcctgtt agcgtaaacc accacataac tatggagcat ctgcacatga
     3541 aaacccgtac acaacaaatt gaagaattac agaaagagtg gactcaaccg cgttgggaag
     3601 gcattactcg cccatacagt gcggaagatg tggtgaaatt acgcggttca gtcaatcctg
     3661 aatgcacgct ggcgcaactg ggcgcagtga aaatgtggcg tctgctgcac ggtgagtcga
     3721 aaaaaggcta catcaacagc ctcggtgcac tgactggcgg tcaggcgctg caacaggcga
     3781 aagcgggtat tgaagcagtc tatctgtcgg gatggcaggt agcggcggac gctaacctgg
     3841 cggccagcat gtatccggat cagtcgctct atccggcaaa ctcggtgcca gctgtggtgg
     3901 agcggatcaa caacaccttc cgtcgtgccg atcagatcca atggtccgcg ggcattgagc
     3961 cgggcgatcc gcgctatgtc gattacttcc tgccgatcgt tgccgatgcg gaagccggtt
     4021 ttggcggtgt cctgaatgcc tttgaactga tgaaagcgat gattgaagcc ggtgcagcgg
     4081 cagttcactt cgaagatcag ctggcgtcag tgaagaaatg cggtcacatg ggcggcaaag
     4141 ttttggtgcc aactcaagaa gctattcaga aactggtcgc ggcgcgtctg gcagctgacg
     4201 tgacgggcgt tccaacccta ctggttgctc gtaccgatgc cgatgcagcg gatctgatca
     4261 cctccgattg cgacccgtat gacagcgaat ttattaccgg cgagcgtacc agtgaaggct
     4321 tcttccgtac tcatgcgggc attgagcaag cgatcagccg tggcctggcg tatgcgccat
     4381 atgctgacct ggtctggtgt gaaacctcca cgccggatct ggaactggcg cgtcgctttg
     4441 cacaagctat ccacgcgaaa tatccgggca aactgctggc ttataactgc tcgccgtcgt
     4501 tcaactggca gaaaaacctc gacgacaaaa ctattgccag cttccagcag cagctgtcgg
     4561 atatgggcta caagttccag ttcatcaccc tggcaggtat ccacagcatg tggttcaaca
     4621 tgtttgacct ggcaaacgcc tatgcccagg gcgagggtat gaagcactac gttgagaaag
     4681 tgcagcagcc ggaatttgcc gctatacctt cgtatctcac cagcaggaag tgggtacagg
     4741 ttacttcgat aaagtgacga ctattattca gggcggcacg tcttcagtca ccgcgctgac
     4801 cggctccact gaagaatcgc agttctaagc aa
//
LOCUS       DAESHI010000199         4799 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.202, whole genome shotgun sequence.
ACCESSION   DAESHI010000199 DAESHI010000000
VERSION     DAESHI010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.202"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..205)
                     /locus_tag="KW011_004176"
     CDS             complement(<1..205)
                     /locus_tag="KW011_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBH7662359.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAV"
     gene            406..1140
                     /locus_tag="KW011_004177"
     CDS             406..1140
                     /locus_tag="KW011_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HBH7662360.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(1142..1753)
                     /gene="emtA"
                     /locus_tag="KW011_004178"
     CDS             complement(1142..1753)
                     /gene="emtA"
                     /locus_tag="KW011_004178"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HBH7662361.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            1853..2767
                     /gene="ldcA"
                     /locus_tag="KW011_004179"
     CDS             1853..2767
                     /gene="ldcA"
                     /locus_tag="KW011_004179"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HBH7662362.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            2862..4598
                     /locus_tag="KW011_004180"
     CDS             2862..4598
                     /locus_tag="KW011_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBH7662363.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
BASE COUNT         1092 a         1218 c         1240 g         1249 t
ORIGIN      
        1 ccaccgccac caggaagctg tgcaaattaa aaccgctgat ggagccgcca atgccaaaca
       61 tggtcgccag ccagccgccg accaccgcac cgactatccc gagaatacag gtcaggaaaa
      121 atccaccacc atcacgcccc ggcatgatta gcttggcgat aatgccggct atcaggccaa
      181 aaataatcca agcaataatt cccatgttca ggccctcgtt tcgatgaatc ggacgcaaga
      241 aatgcttacg tcaacgctct aagtataggc aagggcggga tcgatgctgg gcgtatgtcg
      301 gctgatttaa gaacaacatg taaaaaatat tcacgcattt gtattaagtt ttgttaactg
      361 tgaccgataa accaaagaca gtttgtcagt caggagtttt tccgcgtgag tcattaccat
      421 gagcagttcc tgaaacaaaa tccgttagcc gtcctgggcg tgttacgcga tttgcacaaa
      481 gccgcaattc ctttgcgtct cagttggaat ggcgggcagc tgatcagcaa aatactggca
      541 ataaccccgg ataaactggt gctggatttc ggcagtcaag ccgaagacaa catcgctgtg
      601 ctaaaggcac agcacattac cattaccgcc gaaactcagg gtgcgaaagt cgagtttact
      661 gttgaacaac tacagcagag tgaatacttg cagcttccgg catttatcac cgtaccgcct
      721 cccaccttat ggtttgtaca acgacgccga tatttccgca tctccgcccc actccatccg
      781 ccttattttt gccagaccaa actggcggat aacagtacgt tacgtttccg cctgtatgat
      841 ttgtcgttag gcggcatggg cgcattactg gaaacagcaa agcctgccga attacaagaa
      901 ggcatgcgct tcgctcagat tgaagtcaac atggggcaat ggggtgtttt tcactttgac
      961 gcccagttaa tctctatcag cgagcgcaaa gtgattgatg gcaagaatga aaccatcacc
     1021 actccccgtc tgagcttccg ttttcttaac gtcagcccga cggtggagcg gcaattacag
     1081 cggattattt tctctctcga gcgagaagcc cgggaaaaag cggacaaagt gcgcgactga
     1141 tttacatcgc gtccagtgcc tgctcaagtt tgtagatata gcgcggagcc tgcggcgcag
     1201 ggtgatttcg cgctacgtgt tcgaggaact cgtcagcatc caaatcgttg attttgctga
     1261 tcgccttttt ccgatctgac gagaaagtcc gtagcagcgc acctgccccg ttagcgtatg
     1321 acaccaccag cgcatattgc agtaccttcg gatcttcaat gcctgccagc gggccggttt
     1381 ccagaatatt caggtaagcc gcccccattg aaatattacg ctccggattt ttcagctcgc
     1441 tggtcgtcgg ctcaccactc cagcccatac ggcgataaac atcacgtccg gaggttgaag
     1501 cttttaactg catcaaacca atggcattcg atttactcac cgcattcgga ttaccacccg
     1561 attcgatagc gataatcgcc gtgatcaatt gtggatcgac gccccaggct gcaccggctt
     1621 tctggcttat tggcatccac tgcatcgcac gttgcaccgg aactttcgcg ttccacggcg
     1681 ggttcgtata gtcatgcttt gatgaacaac ccgctaataa cacaatcaaa aaggcaaacc
     1741 atctcaattt cacttcttct atccttatag cctggaatcg ccgttgaggt gacaacggca
     1801 cacctaagcg gcatgatata catttcgttt ctgttgtcag caaggaattg ccatgtctct
     1861 gtttcactta attgccccat cgggttactg cattaaacag cacgccgcgc tgcgtggtat
     1921 ccagcgcctt accgacgagg ggcatcaggt caataacgtc gaggttattg cccgtcgttg
     1981 tgagcgtttt gcgggtacgg aaacggagcg cctggaagat ctcaattccc ttgccagact
     2041 gaccaccccc aacaccatcg tgctggctgt acgcggcggt tacggtgcca gtcgtttact
     2101 ggcagatatt gactggcagg cactggtggc tcgccaacaa catgacccgt tgctcatttg
     2161 cgggcatagc gattttaccg ccattcagtg cggtcttctg gcgcatggca atgtcatcac
     2221 ctttagcggc cccatgctgg tagcaaactt tggcgcggat gaactgaacg cctttaccga
     2281 gcaccatttc tggctggcgt tacgcaatga aaccttcacc attgaatggc aaggggaagg
     2341 cccgacatgt cgggccgaag gcacgttgtg gggaggcaat cttgcgatgc tgatttcact
     2401 gatcggtaca ccgtggatgc caaaaattga gaacggtatt ctggtgctgg aagatattaa
     2461 cgagcaccct ttccgggacg aacgtatgct gttgcagctc tatcatgcgg gaattttgcc
     2521 acgtcagaag gcgatcattc tcggtagctt tagcggcagc acgcccaatg attatgacgc
     2581 aggttacaac ctggagtcag tgtacgcgtt tttgcgttcc cgcctgtcga ttccactcat
     2641 taccggcctc gattttggtc atgaacaacg cacggttacg ctgccgttgg gcgcacatgc
     2701 cattctgaat aacacccggg aaggcactca gttgacgatt agcggtcatc ctgttcttaa
     2761 aatgtaaaaa aatcacgctt cagggctaag taaaacgctg tctctgccgc taatatggta
     2821 gggtttatat aagagacagc gtaatcagga gtaaccgacc gttggatgcc acaacaataa
     2881 ttagcctttt catcttagga tccatccttg tcaccagcag tatattactt agttcatttt
     2941 cttcccgtct tggcattcct attctggtta tttttctggc gatcggcatg ctggcaggag
     3001 tcgatggcgt cggcggtatc ccgtttgata attacccctt cgcctacatg gtaagtaacc
     3061 tggcactggc gattattttg ctcgacggcg ggatgcgcac tcaggccagt tcctttcgtg
     3121 tggcgttagg accggcactg tcgctggcga cgctgggcgt gcttatcacc tctggtttaa
     3181 ccggcatgat ggcggcgtgg ctgtttaatc ttgatttgat tgaaggctta ttaatcggcg
     3241 ctatcgtcgg ctctaccgat gctgcagcgg tcttttctct gctgggtggt aaggggctta
     3301 acgaacgtgt tggctcgacg ctggaaattg aatccggcag taatgatcca atggcggtct
     3361 ttctgacgat taccctgatt gcgatgatcc agcaacatga aagcagtgtc agttggatgt
     3421 tcgtggtcga tattctgcag caatttggcc tcgggattgt catcggactt ggcggtggtt
     3481 atttactgct gcaaatgatt aatcgcatcg ccctgcctgc cggattatat ccattgctgg
     3541 cattaagcgg cggtatttta atttttgctt taaccacggc gctggaaggc agcggtattc
     3601 tggctgttta tctgtgcggt tttctgctgg gtaatcggcc gattcgcaac cgctacggca
     3661 tcctgcaaaa tttcgacggc ctcgcctggc tggcgcaaat cgccatgttc ctggtgctgg
     3721 ggctattggt taacccaagc gatctgctgc ctattgccat tccggcgctc attttgtcgg
     3781 catggatgat attcttcgcc cgtccccttt cggtatttgc tggattgctc cccttccgcg
     3841 gtttcaatct gcgtgagcgc gtgtttatca gctgggtagg attacgcggc gcggtgccaa
     3901 tcatcctggc cgtgttcccg atgatggcgg ggctggagaa tgcacgtctg ttctttaatg
     3961 tcgccttctt tgtggttctg gtttcactgc tcttgcaggg aacatcactc tcgtgggcgg
     4021 cgaaaaaagc caaagtggtg gttccgcccg tcggacgtcc agtgtcacgc gttggtctgg
     4081 atattcatcc ggaaaatccg tgggagcagt ttgtttatca attgagtgcc gataaatggt
     4141 gtgtaggtgc ggcactgcgc gatctgcata tgccaaaaga gacgcgtatt gcggcactat
     4201 ttcgtgataa ccagttgctt catcctaccg gcagcacccg actgcgcgaa ggcgatgtgt
     4261 tgtgtgtaat tggtcgggaa cgcgatctcc cggcgctcgg taaactgttc agccagtcgc
     4321 caccggtcgc gctggatcaa cgcttctttg gtgacttcat tctcgaagcc agcgccaaat
     4381 atgctgatgt tgcgctgata tatggcctgg aagacgggcg agaatatcgt gataagcagc
     4441 aaacgctggg tgaaattgtc cagcagttgt taggcgcggc accggttgtc ggtgaccagg
     4501 tagagtttgc cgggatgatc tggacggtgg ccgagaaaga agataatgaa gtgttgaaga
     4561 ttggcgtacg ggtggctgaa gaggaagctg aatcttaaat ttaatgaagc atctatcgcc
     4621 ggttgcgatg ctttgctgaa cgcagcctga gcctgcaatt gtttgtcgtc cataatttaa
     4681 ccttcatttg atgttgaatt gaacatatca aaatcaggca attacacaaa tctatgtaca
     4741 ggctccatca agggtaaagc gtgatttatc tgaagtcgag ttcgagtcaa caatgaagc
//
LOCUS       DAESHI010000200         4798 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.203, whole genome shotgun sequence.
ACCESSION   DAESHI010000200 DAESHI010000000
VERSION     DAESHI010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.203"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            1..76
                     /locus_tag="KW011_004181"
     tRNA            1..76
                     /locus_tag="KW011_004181"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34..36,aa:Thr,seq:ggt)
     gene            116..231
                     /gene="rrf"
                     /locus_tag="KW011_004182"
     rRNA            116..231
                     /gene="rrf"
                     /locus_tag="KW011_004182"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(294..1052)
                     /locus_tag="KW011_004183"
     CDS             complement(294..1052)
                     /locus_tag="KW011_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBH7662364.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1060..2163)
                     /locus_tag="KW011_004184"
     CDS             complement(1060..2163)
                     /locus_tag="KW011_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBH7662365.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2173..3354)
                     /locus_tag="KW011_004185"
                     /pseudo
     CDS             complement(2173..3354)
                     /locus_tag="KW011_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3422..4454)
                     /locus_tag="KW011_004186"
                     /pseudo
     CDS             complement(3422..4454)
                     /locus_tag="KW011_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
BASE COUNT         1316 a         1240 c         1145 g         1097 t
ORIGIN      
        1 gctgatatgg ctcagttggt agagcgcacc cttggtaagg gtgaggtccc cagttcgact
       61 ctgggtatca gcaccacttt ttaggttaaa gttcggcaga ttagaaaaga atttgtctgg
      121 cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag
      181 cgccgatggt agtgtagggt ctccccatgc gagagtaggg aactgccaga catcaaatca
      241 agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt caattaatgg
      301 attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa aaattcatca
      361 ggtgcagctt gctccactat ttccccacga tccataaaaa tcacccggtc agcgacggtt
      421 cgcgcaaacc ccatctcatg cgttacacac aacattgtca tacccgattg cgccagccca
      481 atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt tggctcatca
      541 aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg ttgctgctga
      601 ccaccagaaa tttgtccggg aaacttatgc gcatgttcgg caattctcac ccgctcaagg
      661 taatgcatcg ccagagcttc agcctctttc tttggcatct tgcgtaccca aatcggtgcc
      721 agggtacagt tctgtagaac ggtcagatga gggaagagat tgaaatgctg aaagaccatt
      781 cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc gatgccatct
      841 actacgatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat ggttgtcgat
      901 ttaccggaac cagaagggcc acacagaacg atccgttctc ccagttgcac ggttaaattt
      961 atatttttca aaacatggaa ttgtccatac catttattga cgttttccag cgtaatcatc
     1021 gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg tacgcccggt
     1081 gttaaaacgt ttttccagat gctggctata gcgcgacatg ctgaaacaga agatccagta
     1141 gatcagtgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg gatcaacggt
     1201 tgcctgctga acgctactga aaagatcgaa caacccgata atgatcacca ggctggtatc
     1261 tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca acgcctgtgg
     1321 cagaataacc agcccctgag ttttccagta acccaatgcc agtgactctg ccgcttcata
     1381 ttgcccttta ggcagcgcct gtaatccgcc tcgcacgact tccgcaacat atgctgactg
     1441 aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atattggtgc cttctgccat
     1501 aaacaaaggc agcatgaccg aagacataaa caatacggta atcagcggta cgccgcgcca
     1561 gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc ggcgacctaa
     1621 cgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa taattaaagt
     1681 tagcgtcagc ccgccccatt gccgggtttc aacccgctcc agaccaaaaa aaccgccata
     1741 catcagccac cagacaatca gtgggtaaat caccgcccag accgcaatat agcgaccgcg
     1801 atgcgggagt attttccaga acattggtgc gattgacaca aggccaatca gtaatgccag
     1861 gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac caaatcgttc
     1921 gtggatgaag acccaacagg caccggcttt tgtgcagtcg gcacgcgttg agccaaccca
     1981 attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca tcaaccatat
     2041 gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc gcacccacac
     2101 catcgcgcgg cttgagttat ggctcgctgg gcgcgagggc tgagacagca atacttttgt
     2161 catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat tcatcagcag
     2221 cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg tctcgatggc
     2281 ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg gatagccaat
     2341 agcggcggca agagaggagt ttttgacgat gttgagatac tggctggtta acggcggaat
     2401 aatcactcgc agtgcctggg gaataatgac ctagcgtagc gtaaccgggt tgggtaatcc
     2461 cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag cgcggataat
     2521 ctcggcgata aatgcagatg tataaaccga aagtgccagc gttaaggctg ccagttcagg
     2581 aattaaaacc atcccgccgc ggaagttaaa gcctcgtagg gctgggacat cccagtgtag
     2641 tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca ctgcggcgat
     2701 cggccaggtg cggcgcagtt gtccggtctt tatctggtat gttttattaa agcggaatag
     2761 cccaacagaa aggactatag ccataacaac agccaggata aacgcaataa atccgtctcc
     2821 cagctgcggt gacggaatat aaagcccacg attgctcaaa aaggccagat caaacgcgct
     2881 cacagcctgg cgtggtccgg gcaaattgcg caacacggca aagtaccaga agaagatttg
     2941 cagcagcggg ggaatattac ggaagatctc aatataaatt gtggaaagct ttcgtagcag
     3001 ccagttatcc gaaagtctcg ccagaccgat aaagaagccc aaaacagaag cgaacacaat
     3061 acacaatgca gaaaccagta gcgtattgag taagccgaca ataaaaacgc gtccgtaggt
     3121 gtcgccctgc tggtaatcga tcaaatgctg gacaataccg aagccagcgc cgcgatccag
     3181 aaaggcaaaa cctgaagtaa tgccacgatt attgagattc gttacagtgt tgtgaaataa
     3241 ccaaccaaca atgccgacaa cagcaacaac ggcaaggatc tggaataacc aggcacgaac
     3301 cgtagggttg gcaaaagaga gtgagccttt aacggttgag cggcgatgag acatagaaac
     3361 ctcggaaagc ataacgctga agagagcgcc gccgtagcga cgctcgttac ctcacaccga
     3421 cttaacgcac gggcggcgcg tactgaatac cgccgttatt ccagagatta ttttgcccac
     3481 gtttaatttt cagcgggctt tctgaaccta cgttacgctc gaaaatttcc gagtagttac
     3541 ccacctgttt gatgatgtta taggcccatt tattatccag cttcagatcc ttgccgtaat
     3601 cgccttcttt acccagcaga tgtgccatat caggcgttgc tggattagcc gctttttcat
     3661 cgacgttctg ggaattgatg cccatctctt cagcattcag cattcagcat ggcgaaaagc
     3721 gtccagcgta caatcgagaa ccattcatca tcgccacgac gaactaccgg cccaagcggt
     3781 tctttagaga taacctccgg taagacaatc cattcagctg ggttgcttaa tttgatgcgc
     3841 agggcataca gttgcgactg atccgaggcc agcgtatcgc agcgaccaga ttccagtgcc
     3901 ttcgctgatt cgtcagagcg atcgaaagtc accggtgtgt acttcatatt gtttgccttg
     3961 aaatagtcgg cgacgttgag ttcggtatct gtacccgcct gaatacagac ggtagcgcca
     4021 tccagttctt tcgcgctttt tagccccgct ttatcgtgcg tcaggaagcc aatgccgtcg
     4081 taatcagtga cgccagtaaa tgccattccc atcccggcat cgcgagatga agtccaggtc
     4141 gtattacggg agagcaaatc cacctcccct gactgtaaag cggtgaagcg ttcttttgca
     4201 gtgagcgggg tatatttcac tttcgtgtcg tcaccaaata cagcagcggc aacaccacga
     4261 caaacatcta catcaatacc tgaaaactta ccgtctgcat cggcataaga gaacccaggt
     4321 aatccatcac tgatcccgca ttgcacaaaa ccttttttct gtacggcatc aagcgtcgcg
     4381 ccagcatgcg cctgatttgc aacggcaagc agcacgctgg cggcagccag tgtggctatc
     4441 atcatctttt tcataatgct tcctgtgagg cgaaaattat cgttatagga cgtttgggaa
     4501 acgtctttta cctgtctgtg gccatcctct tataagcaaa agtagtgcca tcttttgcgc
     4561 acgatttcgc gtcgcaataa gctgagtgta gacaggaaat aataaaaaca gcgccccgca
     4621 atggtgcgaa aatgaaacat gcgccacatt ttggcgcaaa atatttacgc gtgttttact
     4681 aagcagaata atgattgtta gcgtgagtat atttctggtg ggaggtgtga aagttaaatt
     4741 gatgcgttat ttcgaaagca gcgaaataac gcgatataac atgctaaggt aatgactc
//
LOCUS       DAESHI010000201         4701 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.204, whole genome shotgun sequence.
ACCESSION   DAESHI010000201 DAESHI010000000
VERSION     DAESHI010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.204"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            387..1469
                     /locus_tag="KW011_004187"
     CDS             387..1469
                     /locus_tag="KW011_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HBH7662366.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
     gene            complement(1658..2041)
                     /gene="quuD"
                     /locus_tag="KW011_004188"
     CDS             complement(1658..2041)
                     /gene="quuD"
                     /locus_tag="KW011_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HBH7662367.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(2127..2267)
                     /locus_tag="KW011_004189"
     CDS             complement(2127..2267)
                     /locus_tag="KW011_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HBH7662368.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(2264..2626)
                     /gene="rusA"
                     /locus_tag="KW011_004190"
     CDS             complement(2264..2626)
                     /gene="rusA"
                     /locus_tag="KW011_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HBH7662369.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2623..2913)
                     /locus_tag="KW011_004191"
     CDS             complement(2623..2913)
                     /locus_tag="KW011_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBH7662370.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2906..3076)
                     /locus_tag="KW011_004192"
     CDS             complement(2906..3076)
                     /locus_tag="KW011_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662371.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(3076..3531)
                     /locus_tag="KW011_004193"
     CDS             complement(3076..3531)
                     /locus_tag="KW011_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HBH7662372.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            3796..4173
                     /locus_tag="KW011_004194"
     CDS             3796..4173
                     /locus_tag="KW011_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662373.1"
                     /translation="MTNKENKDKYINKFKKELSKYIQPTAGVDIQLFNSKDGGGVIKA
                     TLNRSGKRKSSIAGNFTKLGEAITSSGQRVFGGDLSNVNFYGTNTIFDGDTIFLIKSP
                     DSEEWSSQKASKDVEGIVFGGKK"
     gene            4170..4598
                     /locus_tag="KW011_004195"
     CDS             4170..4598
                     /locus_tag="KW011_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662374.1"
                     /translation="MINITTKDGTQVSGEVRDLVILLETDGKTVSDYLQPIPPKKDTH
                     WSMIFIPVAIYILLNLVYIIAHPLFPEKYLKTSTSIFLFISFILSAIVAYFVFHKHKS
                     ATLSICSLVFLVLIVGANVGFISYSDLTQKATQKLDSLSK"
BASE COUNT         1350 a         1049 c          980 g         1322 t
ORIGIN      
        1 ttttttacgt acgtaaacca ctaaatgata tttgcgagaa tgctaccgag tattgaaaac
       61 accactacaa atacataagc aaatcccaac aaataaccaa caaataattt ccagtgttat
      121 ttttagccgg tttaaattga accttcaaat tatagagcac ttataaataa cagccgttaa
      181 tataaattgg ctaatagatt tatttttatt cagccaagag ccatgaatag gattcgatag
      241 aaaaaagttc agataaaaat agagatctac ttcacaaatc aaacgagaaa ccaaaactta
      301 catcttgaaa taatcacatt gattagatga atatttatcg cgcagtgaca tcatttttta
      361 ataatagttc aaaaaaaggg ctcacgatga aaaaattaac agtggcaatt tctgctgtag
      421 ctgcatcagt actgatggcg atgtctgctc aggcagctga aatttataat aaagacagta
      481 acaagctgga tctgtacggg aaagttaatg ctaagcacta cttctcctct aatgatgcag
      541 atgatggtga tactacttat gcccgtcttg gcttcaaagg tgaaacccaa atcaacgatc
      601 aactgactgg tttcggtcag tgggaatatg aattcaaagg caaccgcgct gaatctcaag
      661 gttcctccaa agataaaacc cgtcttgcct tcgctggcct gaaattcggt gactacggct
      721 ccatcgatta cggccgtaac tacggtgtag catacgacat cggtgcgtgg actgacgtcc
      781 tgccagaatt cggtggtgac acttggactc aaacagatgt gttcatgact ggtcgcacca
      841 ctggtgttgc aacttatcgt aacaacgact tctttggtct ggttgatggt ctgaactttg
      901 ctgctcagta tcagggtaaa aatgaccgca ctgacgtaac tgaagctaat ggtgatggtt
      961 tcggtttctc cactacttat gagtatgaag gattcggtgt aggtgcaacc tatgctaaat
     1021 ctgaccgcac taataatcag gttatctacg gtaacaacag cctgaatgca tctggtcaaa
     1081 atgctgaagt atgggcagct ggtctgaaat atgatgcgaa caacatctat ctggctacca
     1141 cctattctga aacccagaac atgactgttt ttggtaataa ccatattgcc aacaaagcac
     1201 aaaacttcga agtagttgca caatatcagt tcgacttcgg tctgcgtccg tccgttgctt
     1261 acctgcaatc taaaggaaaa gacttgggtg cgtggggtga tcaggacctg gttgaatata
     1321 ttgatgtagg tgcaacctat tacttcaaca aaaatatgtc cacttttgtt gattacaaaa
     1381 tcaacctgat tgataagagc gatttcacga aagcatctgg cgttgctacc gatgatatcg
     1441 ttgctgtagg tatggtttac cagttctaat ttgattacta aaagatatgt tgcgggaggc
     1501 tttgcctccc caacatataa gtggctccct caagccactt cctttaggag cacaaccttg
     1561 cttctaacta tataaacctt ctgttatata ttacccttta tttttggggg cgttgcaacg
     1621 ccccattttt aataattttt agtaaacaat tggcatatta attagagtta ttaacaacga
     1681 tatccatctc taaccggata tctaatgcca ttaacatccc ttcaattatg ccctcagcct
     1741 tctgtaacct tttcccgata taaccatcag agcagcaatg cttacctgcc agtgacatga
     1801 atgtcatacc gactacataa taatctacta ataaatcgtg caaatcgctg ttgttctttt
     1861 tcagacgggc catgcacccg caaataatca tcgcgtcatc gtcacaacat tgcgggcgag
     1921 attttacttt tgaagtaatt aatcccttaa aaccggcggc aatggacgac caggtcacat
     1981 cttcatgatt attagccgcc cacgctcccc aacgctcaag aaccatctga atatcacgca
     2041 tcaacttact ccacaaaaat cagaccagaa cgccaattac aagcaaaaat caacaaaaca
     2101 gtattagttg attgttatct ctgacttcat actcctgctc ctgtcagggt tttggcgtaa
     2161 ttcttcagta ttcggtaatc ggtcaaaaca gaaccgggga aacgatataa gcgcagatgc
     2221 ccccagcggt ggcgaagaag ttctgccata taaaactcaa acatcattca ttccccattt
     2281 cggtgatggt cagttccagc ctcccacctt tggtaacagg catcttcaca acgcggtaat
     2341 caacgacctg agcatcatcc agccagaaac ctgctttggt gagtgcgtca aaagcggctt
     2401 tttgcagatt atccaggtca cggcgacggc gatccggcat gtggcactca atacggattt
     2461 tcactggcat agccaggccg atatccagca ttgcgttttt aatgattcgg gtgacgttat
     2521 cgcggtatgc ctgcccttct gcgctgatgt gtgtgcgccc gcgattatgg cggtaatagc
     2581 ggttattgct cggaggccag ggtaatgtga tgctgtaggt attcacgcct taataacccc
     2641 ctctttcagc cagataacct gcgttctcgc cataccttcc agcgcgcatt cttttgcata
     2701 ctcagcatcg acaaaatatg tgcggcggtc gatttcgtcg tgacaggcag aacatgcaat
     2761 ggtggcaatc aggtctggcg gtttgatacc ggtaccgcac aatccagcca gccggatatg
     2821 tgccagtact gacgtttcag gattgccatt acatacgcca gggattctta cctggcattc
     2881 ccgaccacgc gctgcttttc tcaaatcagc catgattcct ccttgctgcc agtcgcaacc
     2941 attttttatc aaccaggcta gcggtatatc cgagcagtgt tggtatttcg gatggcttca
     3001 gctcaggctt acgcttacga cgatttgata ctctgtagat gtgcccgttc atgacacgaa
     3061 taagcggtgt agccattacg cctcctgctt atcgcgcagc tgctggtatt cacagctctg
     3121 cggaattgtc aggtggcagc cgatattcat cgcccaggct tcgactttgc acaggaagat
     3181 gtacatctcg ccggtttcca gctctgacgt atggcggagg gattggacgg tggtgacctc
     3241 gccggacacg acatctacac ggtctttgct ttcgtagccg agataggtgt gcttcatcgc
     3301 gtctttgacc cactcaggcg tagcgaaggt cttgccgcgg gcgatgagat attcgctgat
     3361 ctccgtgtac cacatgtggc tgagcgcgtt ctgcgacagg ctgcgcttct cgcgccacgg
     3421 cttaacctgc aggcgtaagc attgcccggc atccagcaat ggctgaatct gctggccgat
     3481 ggccgcgaag ttaccgcgat ggagtttgat gccgtctact ggcagagtca tacggcctcc
     3541 ctaacggaaa ccgcagaatg cagaaaatcg caggtgcatt tctgcatctg tgacaaggtg
     3601 atgagttcag attgtggtcg catttaagtc cccttaaatg cgcagaagtc accggagttg
     3661 ttcaggctcc gatgagagga ttatggcgga ttgattacga taaatcaaaa ctatcttgaa
     3721 gccaaaacag tgatcttcgc tttaaaaacc ttgtatactt tcacctaatc acagccagaa
     3781 ctatggatct aaccaatgac caacaaagaa aacaaagata aatatattaa caagttcaag
     3841 aaagagcttt caaaatatat acaaccaact gctggagtgg acattcaatt atttaactct
     3901 aaggatggtg gtggtgtaat taaagccact ttgaatagaa gtggaaaaag aaaatcatct
     3961 attgctggta attttactaa attaggtgag gcaattacct cgagtgggca gagggttttt
     4021 ggtggtgatc tatcaaatgt gaatttttac ggaacaaata cgatttttga tggagataca
     4081 atatttttaa ttaaatcacc tgattcagag gagtggtcat cacaaaaagc ttccaaagac
     4141 gtagagggca tcgtatttgg agggaaaaaa tgattaacat tactactaag gatggcactc
     4201 aagtcagtgg tgaagtaaga gatctagtaa ttcttcttga aactgatgga aaaacggtgt
     4261 ctgattattt gcagccaatc cctcccaaaa aagatacaca ctggagcatg attttcattc
     4321 cggtagcaat ttatatacta cttaatcttg tatacattat tgctcaccca ttatttcctg
     4381 aaaaatatct aaaaacatca acatctatat ttctattcat ttccttcata ttaagtgcaa
     4441 tagtagcata ttttgttttc cataagcata aatctgccac tctatccata tgcagcttag
     4501 tgttcttagt gttaattgta ggtgctaacg tagggtttat cagttacagc gacctaacac
     4561 aaaaagcgac gcaaaaatta gatagtttgt ctaaataatt atgttgccat gcgaagtatg
     4621 catggctgca tgtctgcctt ccatttaaaa tgggctaaga cctataaccc taaatattat
     4681 tctttattat cttctttacc a
//
LOCUS       DAESHI010000202         4519 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.205, whole genome shotgun sequence.
ACCESSION   DAESHI010000202 DAESHI010000000
VERSION     DAESHI010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.205"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..264)
                     /locus_tag="KW011_004196"
     CDS             complement(<1..264)
                     /locus_tag="KW011_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662375.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAP"
     gene            493..1101
                     /locus_tag="KW011_004197"
     CDS             493..1101
                     /locus_tag="KW011_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HBH7662376.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1199..2590
                     /gene="selA"
                     /locus_tag="KW011_004198"
     CDS             1199..2590
                     /gene="selA"
                     /locus_tag="KW011_004198"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBH7662377.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEIIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2587..4431
                     /gene="selB"
                     /locus_tag="KW011_004199"
     CDS             2587..4431
                     /gene="selB"
                     /locus_tag="KW011_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBH7662378.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT         1017 a         1143 c         1330 g         1029 t
ORIGIN      
        1 cggcgcgggc gtttttgtcc ggtaactgct gtaggtgcgg gagaggatga acggcagcgg
       61 ggcgggcagg gcgatgtcgg tttcaccggg aaggaccttt gcaccgagca ggggattgac
      121 cggatggccg gacgtcactc cgccggggca caccgaacag gccacaccgg tgggggcacc
      181 gatgcgcacc ccggctgaac cctgaacaat gctaccgcca tactgcgtca tgtcgccctg
      241 acgcgctgcc ggttttccgc tcatctgctc atccttgttt tatcgggtta tttgtctgct
      301 tccaggcaaa accctacaga tttataagaa agccttaagt gagatatcca tcagatagaa
      361 acaattagga aaatatttat atcaccatga taaatatgtg atttatttgg aagtaacaaa
      421 ccatcaacat tttctgccct ttttcccatc accaggccat actgatattt ttcctcgcac
      481 aaaggactcg gcatgaaact cgtcggtagc tacaccagcc cgtttgtacg caaactttct
      541 attctgttgt tagaaaaggg cataactttc gaatttatta atgaactgcc ctataacgcg
      601 gacaacggcg tggcgcaatt taacccgtta ggaaaagtgc cggtgctggt gaccgaagag
      661 ggcgaatgct ggtttgattc gccgatcatc gctgaatata ttgagttaat gaatgtcgct
      721 ccggcgatgt tgccgcgcga tccgctggag tcgttgcggg tgcgcaaaat tgaggcgctg
      781 gcggatggca ttatggatgc cgggctggta tcggtgcgtg aacaggcgcg tccggcggcg
      841 cagcagtctg aagatgaatt gttacgccag cgggagaaaa tcaaccgcag tctggatgtg
      901 ctggaaggat atctggtcga tggcacactc aaaaccgata cggtcaatct ggcgactatc
      961 gccattgcct gtgctgtcgg atatctcaat ttccgccgcg ttgcgccagg ttggtgtgtc
     1021 gaccgcccac acttagtcaa actggtcgaa aacctgttta gccgcgaaag ttttgctcgc
     1081 accgaaccgc caaaggcttg atgcgcgata tgtcctcctg acccatctca cgttacaatc
     1141 cgtggttatg ttaaacgccc ttctccgtgt gagagggcct tgatcagcca ggtttcctat
     1201 gacaaccgaa acgcgttccc tctatagtca acttccggct attgatcgct tattgcgcga
     1261 tagctccttc ctttctttgc gtgatactta tggtcacacc cgcgtggtgg aattgttgcg
     1321 tcagatgctc gacgaagcgc gagaagtgat tcgtgacagc cagacgcttc ctgcgtggtg
     1381 tgaaaactgg gcgcaagaag tcgatgcccg gttgacgaaa gaggcgcaga gcgcgctgcg
     1441 tccggtgatc aacctgacgg gaaccgtgct gcataccaac cttggacgag ctttacaggc
     1501 ggaagccgcg gtggaagccg ttgcgcaggc tatgcgttcg ccagtgaccc tcgagtacga
     1561 tctggacgac gccggacgcg gacatcgcga tcgggcgctg gcgcaactgc tgtgccgtat
     1621 tacgggggcg gaagatgcct gtatcgtcaa taacaatgcg gcggcggtgt tattgatgtt
     1681 ggcggccact gccagcggga aagaggtggt agtatctcgc ggcgaattgg tggagattgg
     1741 cggcgcgttt cgtattcccg atgttatgcg tcaggcaggc tgcaccctac acgaagtagg
     1801 gaccaccaac cgcacgcacg cgaatgatta tcgtcaggcg gtgaatgaaa ataccgcact
     1861 gttgatgaaa gtacatacca gtaactacag cattcagggg ttcaccaaag cgatagatga
     1921 agcggaactg gtggcgctcg gcaaagagct ggatgttccc gtagtgactg atttaggcag
     1981 tggctcgctg gtcgatctta gccagtacgg tttgccgaaa gagccaatgc cgcaggagtt
     2041 gattgcggcg ggcgtcagtc tggtgagctt ctccggcgac aagttgttag gcgggccgca
     2101 ggcagggatt atcgttggta aaaaagagat aatcgcccgc ctgcaaagcc acccgctgaa
     2161 gcgtgcatta cgcgcggata aaatgaccct cgcggcgctg gaagccacgt tgcgtcttta
     2221 tttacaccct gaagctctga gtgaaaaatt accgaccctg cgcctgctta cccgcagcgc
     2281 agaggtcatt caaatccagg cacaacgttt acaggctccc cttgccgcac attacggcgc
     2341 ggagtttgcg gtacaggtta tgccatgtct ttcgcagatt ggcagtggtt cgctgccggt
     2401 tgatcgcctg ccgagcgcgg cattaacgtt tacaccccat gatggacgcg gtagccacct
     2461 tgagtcatta gccgcccgct ggcgtgaatt gccagtgccg atgattggtc gtatttatga
     2521 cggacgattg tggctggatt tacgctgcct tgaagatgag caacggtttt tggagatgtt
     2581 gttgaaatga ttattgcgac tgccggacac gttgaccacg gcaaaacaac cttattgcag
     2641 gtgattactg gcgtaaatgc tgaccgtctg ccggaagaaa aaaagcgcgg catgaccatc
     2701 gatctcggct atgcctactg gccgcagccg gatggtcgcg tgcctagttt tatcgacgta
     2761 cccggtcatg aaaagtttct ttccaacatg ctggcgggcg ttggtggtat cgatcacgcg
     2821 ctgttggtgg tggcgtgcga tgacggcgtg atggcgcaga cccgtgagca tctggcgatt
     2881 ttgcagctga ccggtaaccc gatgctgaca gtggcgctga ccaaagccga tcgcgtggac
     2941 gaagcgcgtg ttgatgaggt tgaacgccag gtaaaggagg tgctgcggga atacggtttt
     3001 gctgaggcaa aactgtttat caccgcagca accgaaggtc ggggaattga tgccctgcgc
     3061 gagcatctgc ttcagttacc aggacgtgaa cacgccagcc aacatagtgt ccgcctcgcg
     3121 atcgaccgcg catttaccgt aaaaggtgcc gggctggtcg tcaccggtac ggcgttaagc
     3181 ggggaagtga aggtaggcga ttcactctgg ctgactggtg taaataaacc gatgcgtgta
     3241 cgtgcgctgc atgcacaaaa ccagccaaca gaaaccgccc atgccgggca gcgtatcgcg
     3301 cttaacatcg cgggtgatgc ggaaaaagag cagattaacc gtggcgactg gctgcttgcc
     3361 gatgcgccgc cagagccgtt cacacgggtg attgtcgagc ttcaaaccca tacgccgctg
     3421 acccagtggc agccgctgca tattcaccac gccgccagcc acgttacggg acgcgtttcg
     3481 ctgctggaag ataaccttgc cgagctggtg ttcgacacgc cgctgtggct ggcggataac
     3541 gatcgcttag tgctgcgcga tatctcggcc cgcaacacgc tggcgggggc gcgtgtggtg
     3601 atgcttaacc cgccgcgtcg cggtaaacgt aagccggaat atctgcaatg gctggcgtct
     3661 cttgcacggg cgcagagcga tgccgatgcg ttatctgttc atctggaacg cggcgcggtt
     3721 aaccttgcgg atttcgcctg ggcgcgccag ctcaacggcg aagggatgcg cgaattgctg
     3781 caacagcctg gctatattca ggctggttat agcttgttga atgcgccggt tgccgcccgc
     3841 tggcagcgga aaattctcga cacattagcg acttatcatg agcaacatcg cgatgaacct
     3901 ggtcctgggc gcgaacgtct gcgacgtatg gcgttgccaa tggaagatga agcgctggta
     3961 ctgttgctga ttgaaaagat gcgcgaaagc ggcgacatcc tcagtcatca cggttggctg
     4021 catctgccgg atcacaaagc gggcttcagc gaagagcagc aggccatctg gcaaaaagca
     4081 gagccgctgt ttggtgacga gccgtggtgg gtgcgtgatc tggcaaaaga gacgggtacc
     4141 gacgagcagg caatgcgcct gacgctacgc caggcggcgc agcaaggaat aattaccgcg
     4201 atcgtcaaag atcgttatta ccgtaacgat cggattgtcg aatttgccaa tatgatccgc
     4261 gatctcgatc aggagtgtgg ttcaacctgc gcggcggatt tccgcgatcg cttaggcgta
     4321 ggccgaaagc tggcaattca gattctggaa tattttgacc gcattggctt tacgcgtcgt
     4381 cgtggaaatg atcatttatt gcgcgacgca ttattatttc cggaaaaata aggaaatgat
     4441 taattaattt ttaaaataaa ttaatacaaa tttctggtaa tgactccaac ttattgatag
     4501 tgttttatgt tcagataat
//
LOCUS       DAESHI010000203         4482 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.206, whole genome shotgun sequence.
ACCESSION   DAESHI010000203 DAESHI010000000
VERSION     DAESHI010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.206"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(7..1068)
                     /locus_tag="KW011_004200"
     CDS             complement(7..1068)
                     /locus_tag="KW011_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HBH7662379.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(1065..2462)
                     /locus_tag="KW011_004201"
     CDS             complement(1065..2462)
                     /locus_tag="KW011_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HBH7662380.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQLGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2617..3615
                     /locus_tag="KW011_004202"
     CDS             2617..3615
                     /locus_tag="KW011_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBH7662381.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3726..>4482)
                     /gene="ompG"
                     /locus_tag="KW011_004203"
     CDS             complement(3726..>4482)
                     /gene="ompG"
                     /locus_tag="KW011_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HBH7662382.1"
                     /translation="AEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEV
                     HYQFLENDDFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGW
                     LSMYKFANDLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFS
                     TQEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNG
                     LSVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         1093 a         1229 c         1099 g         1061 t
ORIGIN      
        1 attgcattat ttttcgctgg gtgttttgcc tttttgcagt gtttctttca cctgaccgat
       61 aagctgacga cggaaatccc cgaggcgagg tttgtcatcg tcaatccacg gcagcgggcg
      121 gcaaagctcc atagctttaa tcccgagtcg tgccgtcaga agtcctgcac caatcccctg
      181 agctgcgcgg gtagacaaac gagcagcgag atcttgcgac atccagtcca tccccacttc
      241 gcgcaccagt tcgctggctc cggcaaaagc gatattcagc aataccagct taaacagacg
      301 caaacggctg taatacccca gttcaatgcc atacagcgtg gcgatgcgat taattaaacg
      361 cagattgcgc caggcgataa acgccatatc gaccaacgcc agcgggctga ccgcaatcat
      421 caacgttgat tccgccgccg aacggctgat ttcgcgccgc gcctgggcat ctaaaactgg
      481 ctggaccaaa tgcgcataca ggctgaccac ttcacggtcg ttttgcgttt catggattga
      541 ggcataccag cgttgcagcg ccggatgcga ctgatcaata cccgcctgct gcgccagttt
      601 ttcgcaaaat gcgcggcctt tgcccgtgcc gtggctatgc aataaatcac gcgcttcgtc
      661 gcgttcatgg gcgcgctgtc gcaagcacca taagcactgc cactctgtta ccacagaacc
      721 tacgccagcg ccgataatca atgccccagc ggcacatcca cccagcgcta cccagtcctg
      781 ggtttgccag gcattcattg tccactgtac accctgcccg acaacacttg cgccaaacag
      841 agccagcccg cccatcacca ttttgcgcca caggctgcgt ttcggacgta acgctgcatc
      901 cattaccgct tcaacttgcc cctcttcttc aggcgcttca tcgagcgtgg ccggggcaaa
      961 attttgcgcc tgattttcgt caaaggtctg ctgcgccctg aattttggat tctgatcgac
     1021 ctccagcgga ccgtcgaaat caatacgtgg ttttaacggt tcggtcatcg caatttatct
     1081 cctattaaaa attccagcgc ggcatcaaga cgaatatgcg gcagcggttt gtcgacatcc
     1141 atcacctgcg ggcgaaacgc ttcaaactgg aacccttgct tgtcccagaa cgcctgaccg
     1201 ggcaaacgcg caggaacttc accggggtaa acggtgagag gcgcaccatc gctgagccga
     1261 ttaccgcgca atgccggaat tttctcgccg ttgacatcaa taatgccgct ggtggtcgcc
     1321 tgcaccgagg ccagccccag gcaatccata ctaatccctt caaacgccgc attttgccag
     1381 gcatcctgaa tcagttgttg cagtaatgaa accatattgg cgtgctgatc gaccgttaca
     1441 tggtccgctt tcgttgcggc aaacagcagt ttgtcgataa ccggcgagaa taagcggcga
     1501 aagagcgtgc gctgcccgta gtggaaactt tgcatcagct gcgtcagtgc cagacgcata
     1561 tcattaaatg cctgtggccc actgttgaga ggttgcaggc aatccaccag cacaatctgg
     1621 cggtcaaagc gcagaaaatg attcttatag aaccccttca ccaccttctc gcagtaataa
     1681 ttaaaccgct cgcgcagcat tccggcattg gtgtgcttat cggcctgcgc cagtttggac
     1741 tcgccccagg tatcgacatc cggccacggg aagaattgca gcgcgggcgc acctgccata
     1801 tctcctggca agacaaagcg ccctggctga ataaagtgca gccccagctg tttacagtgg
     1861 tggagataat cggtccacgc ggcggcaatg tctgccagcc ggttttcgtc ggcaggtgct
     1921 agcgggtcca gcccttcgca catcattcgc catttgaccg accattcgcc gcgctgacca
     1981 ttgagtaagc ccgtcatctg gcgtgaccag cttaaatagt cctgcgccag catcggcagg
     2041 tcgagcaacc attcgccggg ataatccaca atctccagat acagcgtgga ggtgtctttg
     2101 aagtggcgta gcagcgaatc gttcgattta aaacgtagcg ccaggcggat ttcactgacg
     2161 ccgcgcgtag gcgtcggcca ggcgggagga tcgccatata actgcgccag tccttcatca
     2221 taggtgaagc gcggaatgcc aaagtcacgc tgaggaatgc gttttacgcc cagcaggcgc
     2281 tcttcacgca ccgcgcttaa cagcggcaag cgtgctccgg cgtgaatatt gagcaactga
     2341 ttgaccatcg cagtgataaa cgctgttttg ccgctgcggc ttagtccggt tacggcgagg
     2401 cgcagatgtc tgtcgacacc ccgattcacc agcgcattaa gttcattttt aagtcgcttc
     2461 attgctgtcc tgtgctgctc tgaagaccag aaaaaaatca ttcgtaagga tatgaatgtg
     2521 ggggccaggc cattgattat caactgcccg tgttgtcatc gacgggcgaa ctccataaga
     2581 tgtgcagcat tcacgttcgg acggagttcc tgacctatgt cccctactat ttatgatatt
     2641 gccagggttg caggcgtatc aaaatccacc gtatcacgcg tgctgaataa gcaaactaat
     2701 atctccccgg aagcgcgcga aaaagtgtta cgggccattg aagaattaca gtatcagcca
     2761 aacaagctag cccgcgcgct gacctcttcg ggttttgatg ccattatggt gatttctacc
     2821 cgttcgacca aaactacggc gggtaatccg tttttctctg aagttttgca tgccatcacc
     2881 gccaaagctg aagaagaagg tttcgacgtg atattgcaga cgtcgcacaa cccggcagaa
     2941 gacttacaaa aatgtgaaag caaaattaag cagaaaatga ttaaaggcat tattatgcta
     3001 agttcgccag cggatgagtc attttttgcc caactcgata aatacgatat tccggtggtg
     3061 gtgattggca aagttgaagg tcaatatgcc catgtttatt ctgtcgatac cgataatttt
     3121 ggcgacagca ttgcgttgac cgatgcatta attgaaagtg ggcatcaaaa tattgcctgc
     3181 ctgcatgctc cgcttgatgt ccatgtttca gtggatcggg taaatggtta taagcagagc
     3241 ctggctgcgc ataatattgc agtgcgtgat gaatggattg ttgacggtgg ttatacccat
     3301 gaaacagcac tgcaagccgc gagagaatta ttaagccagt caccgttgcc tgaggcggtg
     3361 tttgccactg acagtctgaa attaatgagt atttatcgtg cggcagcaga gaaaaatatt
     3421 gctattccgc agcagttagc ggtggtgggt tatagtaatg aaacgctgtc gtttatatta
     3481 acgcctgcac cgggcggcat cgatgttccg acgcaggagt taggtcaaca aagctgcgag
     3541 ttattattcc gcttaatttc tggaaaaccg tcaccacaga atattaccgt tgccacgcat
     3601 atgacgctga aataaatacc agctaccttg cctgaatgcc ggatgcgatg ctagcgcatc
     3661 ttatccggcc tacatttata gatcggataa agtactcgcg ccgcatccgg caatttagcc
     3721 cattatcaga acgagtaatt tacgccgaca cctgcataat ggaatttatc actgtctcct
     3781 tcgtcgtgat cctgccactc aaacgcgtat tccagcgaaa cggaaagtcc gttctggaaa
     3841 tcataaccgt aaaataaacc tacacggtta aaatcatggc cttcacgttc aatatcatcc
     3901 tgccagtccc agttactcca gcgatccagc ccaatgcgcg tatacggcgt caccgagtgg
     3961 ttgccgagcg tcagcggcaa ataggcgcga atttcttgcg tggaaaactc accgttattg
     4021 cggctgtcgt ccatattgaa gccgcgctcg agataatagt tcactcgcaa ggcaaccgtt
     4081 tcgttgaagg tatattgcag acctgtttcc gtttcgacac gggtatcagc gtaaccggta
     4141 gtgttcagat cgttggcaaa tttatacatc gacaaccaac cgttgaaacg taaatcgtca
     4201 gtcagtttca catcccagtc tggcgcgatt ttccagcgct gcatattcgc cgtgtcttta
     4261 cccggttcat caacgtagtg ataaccataa ttacggaaac cgccggtcag gccgaaactg
     4321 aaatcatcgt tttcgaggaa ctgataatgc acctccagct ccgggcgatc aaaccacgtt
     4381 ccacgtttac ccgcgctata atctaccggc ccttcctgat aataggccag agcaattctc
     4441 cacggcccgt tggcggcatt aaaatagact gaaggctccg cc
//
LOCUS       DAESHI010000204         4409 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.207, whole genome shotgun sequence.
ACCESSION   DAESHI010000204 DAESHI010000000
VERSION     DAESHI010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.207"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(173..2122)
                     /locus_tag="KW011_004204"
     CDS             complement(173..2122)
                     /locus_tag="KW011_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBH7662383.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2207..2542)
                     /locus_tag="KW011_004205"
     CDS             complement(2207..2542)
                     /locus_tag="KW011_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HBH7662384.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2542..2898)
                     /locus_tag="KW011_004206"
     CDS             complement(2542..2898)
                     /locus_tag="KW011_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBH7662385.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2898..4394)
                     /locus_tag="KW011_004207"
     CDS             complement(2898..4394)
                     /locus_tag="KW011_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBH7662386.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT         1021 a         1313 c          970 g         1105 t
ORIGIN      
        1 aacgttacta ataaatcgtg aaacaatctc ctcatttgtt agtattttcg cctgagcaca
       61 gcaagaggtt aatattgcaa ccgtcattaa aaatggcttt atcacaaaac aaactccatt
      121 gttagcatgt tacgcgataa ttttgcctca tctttcacca caaaaaaaga actcaccttt
      181 taaatggaga atacccaaca tcatgggtga ttttcataaa gggatcgcct gttttcggca
      241 aatcgataac gcgcattcca ggcggcgcgt tgtcaaacgt caccttaagt tcgctgcggg
      301 ttgccggtga agatgcaagg cttaacagct gattcctttc cagtgataca ttcggggtgt
      361 agcgtttatc gggagataaa tacccatcac cacgtggcat ctgccatccg gtcatgtcat
      421 agacaaaatc atggaactgt ttgccccact cgtcgaactt atcattcaga ccaaatccac
      481 tgttcagtgc atcagccaca aaatttttaa caagccaggg atgtgctttt tcaaactcct
      541 gcgccatcat gcccagttca agcaatccac caatcagact gatctttccg aggcccttta
      601 atttcagccc tgtttttcct gccgccgttg tccattgatt ctgggcgact gttgccgcac
      661 gaatacttgc caccgccttc acaccaatat acagggcaga aaccgttgct attgttttta
      721 ctgcctgttc ccatcccccc attgctctga caacctgatc aacctcctgc cagacttttt
      781 tcacaaccgg accgaccgtt tcccagttat caattatgag atatgcacca ccgacgagaa
      841 gcgcaattaa tccctttgcc ggcgtcatat tcatcacgct acccatgatt tttgttatgc
      901 gtgtcaatgt gccaatcgcc acgcccatcg tcagtaatgc cgcaccagtt ttcgcgatgg
      961 tttttacgac ctcaggattt tctctgacaa acgtgcgcac ttcttccaga aaaggcttca
     1021 tctcttttat gccttcattg agtgaaggca ggaaggtttc ccccagcgtg gaagaaatcg
     1081 cattgatctg gttctgcagc agcaacaact gattttctgt cgtcgcggca cgggcggcat
     1141 attctttctg cattgagccg ccatactgct gcgcatccgc cacacgatta aagttggttc
     1201 gcaacaaatc cagattcgtg agcagcggcg caatggcccc cagggattcc ttcccgaaca
     1261 gcgctttcag cacagcagcc tgtttttctt tcggcacttt tgccagagaa tccagtacgt
     1321 gcagcatggc cccgcgggcg tctttctgca tatccgccgc cagttttttc gggctaatgc
     1381 gcaacgagcg gagcactttt ttctgcgatt tggtggcaga atccctcgct gtcagcgaca
     1441 acataaaatt ctttatcccc gtcgcagcaa tttctgactc cacccccatt ccggcaatgg
     1501 ttgcccccat tgcggcaatc tctccggagg ccacacccgc aacactgcct aaagggccaa
     1561 tacgggtcac aacatcagaa atctttttcg cacttgcagg accggtatta ccaagatagt
     1621 taatcttatc cgcaagccct gccacctctc cctgtgtcag tttaaaggca gtgcgccact
     1681 gcgccatcat ctgaccggat tcttctgccg tggtgtcaaa ggccacgccc atcttcacgg
     1741 catcgtcagt aaactgcatc agctcgtcac gggcgatacc agcctgacca cccgccgcca
     1801 caatttcggc gataccttcc gccgacatgg gcagttctgt tgacaggtcg cgcacctgct
     1861 ccgtcattgc cttaaacgct tccggcgtat ccagaccatc caccactttc cggacatccg
     1921 ccatttttga ttcaagggca atggcagatt tgaccgggag cgccagcgcc cccagtactg
     1981 cggttccggc acccgctgcg cccagtaaca ggctggcaaa ctccttttta aaacctttca
     2041 gctggcgctg cattcccttc agcggtgccg ataccttatc cacggcagtg atgatcgcct
     2101 tcagctgaaa actgtcagcc atgcttcatc tcctcgttaa tgcggacggc ctcggcttcc
     2161 agttctgtaa actgcgaaac agccccccgt cgcagctcca gtggattcag tttccagaac
     2221 caggcaacat tgtagagtcg tttccggagg tgcttcccgt ctccgactgg gtaaaaaaac
     2281 gcaggatctg catgctggtc ttaaaaatat ccagttttgc catctgcgcc gccgatgaac
     2341 gcgggatccc cgccagcaac gggatatatt tcagtgccac ctggctgtcc agtttaatac
     2401 tgccttcacc ggaaataatg aaaggaaaac ccagcgcctc gatttcgtca tacgtgggct
     2461 cgcgcaactc cagcacatgc agcatttcgt tatgcgctgt caccggtttt tttaaaacta
     2521 tttctgtaac gttcattgcc attactgata ccctccctct tcgccgtgga attcaagatc
     2581 tgccgtgcct tcttcggcat tatgattagc ctctccgtgc agccatgccg ccgaaagcac
     2641 atacaccatg ccgtttgcca gctcagcggt aatcgtcatc tggtctgagg tggtaatttt
     2701 gtcgaccgga aagtttttgg gcactttaaa cgtgccctta acgtaaggtg accgccaggt
     2761 ctccttgtaa tccacatccc ctgccatgcc gacgatgtca tcccggacat tggtgttcat
     2821 cggcacttca atgccacctg tcagcgataa ctgctgacca tccactttaa aaaaacaggt
     2881 tccggcgatc tttgccatta tgctgactcc tctgaatact gaagacggaa ctggttaacc
     2941 accgcaaaga cacgtaactg attaacataa tccggcggga acagcgtatt cagtcggttc
     3001 gggttatctg catcacgctc aactatcaga tactgtttaa acagatcgta attttccaca
     3061 ataccggcac gctccatctg acgatatgtc gccagaagtt cccctttgat aacggcagga
     3121 gtgacaatcg cctgccccgg accaaaacgg gtaccatcat ttgccagctt gtgacgtccg
     3181 tacttgcttg tgatgaccga tttcagtttg cgaagaacat atgcactggt atgcagagtt
     3241 tcactgtcca gatagctgtt gtctgccacg ccatacgcat tctttttgta ggtggttacg
     3301 gaacgctgga tctgcaacgt gccgccttcc acataagccg tcgccacgcc gtgagataaa
     3361 agggtctgct gctctgtcat gatgaatcgc ttacctttcg gtgccggaag catccccacc
     3421 agctcccccg tctgtgtcgg acgggcagga tcattccgga taaataccgc ttcacgggca
     3481 aggcgactgg caaccagttc atcgacaggc gactgggttt ctttttcgta accggcaagc
     3541 gtgatatgtt gctgattatg catatctccg gcaccaacca gctctgacag cgttcccagt
     3601 tttgcggtat agacatgccc gtataactgg cgcgcataac tccagcgacc gctgctgtca
     3661 ttcatttcgg tcatcatcat attgatggag gcggcatcgt tgaacggcag accgataaag
     3721 tcgaatgcct catcgcccat agcagcaaca gcggcggtaa gatcaggcgc tccgctaccg
     3781 gcagcaccga cttccgtcac cacccgaagc cctgctggca gaatctcacc accaccagaa
     3841 ccataataat tcagacagac cggtaactca ttaccataca gccccttatg acgggcagtc
     3901 agcgtcacca cacctgcatc agatgacgcc gtaaacggca gggtgataac cccgtttacc
     3961 gcttccttaa tcgcggtggc aaccgcagtg gcatcatcgc cattcaccac aggcacctgt
     4021 acacgggagc gcccgacata cagactcagg gtgccgcttt cctctgcttc tccggcaacc
     4081 gtcaccctga ccgtcgccgc cgcccctctg gcttccggta ctgcaataac atacagttca
     4141 ccgaaaggat ctgtctgacg gtagacttcc accatacgcg ccagctggct ccccgcccca
     4201 caaatctggc gggcataatc tgccgatggc atcagcacca ggctgttaac ctcaatggct
     4261 gcatcgttgc tggcatgccc gatcagtaat gcaggcgcgc tggtcaccgc tgtatttgcc
     4321 gcagaattgt tcatctcggc gtagaacaac ggcaccagcg tattcgacgg aacagtatta
     4381 aaacttatcg tcatgatttt ttagcctta
//
LOCUS       DAESHI010000205         4395 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.208, whole genome shotgun sequence.
ACCESSION   DAESHI010000205 DAESHI010000000
VERSION     DAESHI010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.208"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..590)
                     /locus_tag="KW011_004208"
     CDS             complement(<1..590)
                     /locus_tag="KW011_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBH7662387.1"
                     /translation="MADSQFYRPGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQ
                     AAAVHTLYGYIDYLARNLLPDVCDEDWLYRHARIKRCPRKEAVAARGFVRWDGVEGTP
                     VLPAGTQIQRDNQVTFTTTATVTAADGLLRVPVVADEPGSAGNTDDGIAMQLGTPVSG
                     LPSTGYADTITDGEDIENLEIWRARVMERYYYIPQGG"
     gene            complement(583..993)
                     /locus_tag="KW011_004209"
     CDS             complement(583..993)
                     /locus_tag="KW011_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HBH7662388.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHNIIFDDIWSELNG"
     gene            complement(999..1532)
                     /locus_tag="KW011_004210"
     CDS             complement(999..1532)
                     /locus_tag="KW011_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HBH7662389.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            complement(1510..2586)
                     /locus_tag="KW011_004211"
     CDS             complement(1510..2586)
                     /locus_tag="KW011_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HBH7662390.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLGENILSCDTERSIRERFSEYQVSGQRPGNDDD
                     FGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALTRETTYT
                     VQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRVGPADAY
                     LPEPVTARKKKMLRRISDERVFSS"
     gene            complement(2583..3974)
                     /locus_tag="KW011_004212"
     CDS             complement(2583..3974)
                     /locus_tag="KW011_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HBH7662391.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVF
                     LDELIEDMFGDFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDL
                     SVILMPPSVASDFVHTLQDTWRAGTRLVDNTQDLVQSITTMSGITLDPGLAPRAVWPT
                     DSASVIRQKQQTNLVAAVIRTTAISEATRAVTSLPQPGSLVKNQQAVVAVGGSTERQS
                     DIIHVSHPALDSVAASTGQDERAQPPTRENLTIIRESLNAAIEQELRRTADDRLFFQL
                     TSLRTELNRDIQARLVQTEETAERTPAEVLPALVLAASWYDDASRETDILDRNAISHP
                     GFVPVRALRVPVR"
     gene            complement(4021..4227)
                     /locus_tag="KW011_004213"
     CDS             complement(4021..4227)
                     /locus_tag="KW011_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662392.1"
                     /translation="MTFLIPIYKDDDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAG
                     VNVNGESGVTYENCKLNMKDINDD"
BASE COUNT         1052 a         1281 c         1039 g         1023 t
ORIGIN      
        1 cccccctgtg gaatgtagta ataacgttcc ataacgcggg cacgccatat ttccagattt
       61 tcaatatctt caccgtctgt aatggtgtca gcgtaccctg tggacggcag accactgacg
      121 ggtgttccca gttgcatggc aataccatca tccgtattcc ccgccgatcc cggttcgtct
      181 gccacaacag gcacccggag aagaccatcg gctgcggtca ccgtcgccgt cgtggtaaag
      241 gtcacctgat tatcacgctg gatctgcgtt cccgctggca ataccggcgt cccctctacg
      301 ccatcccagc gcacaaatcc ccgggctgcc accgcttctt ttcgcgggca gcgtttgatt
      361 ctggcgtgcc ggtacagcca gtcttcatca cacacgtccg gtaacagatt tctggcaaga
      421 taatcgatat acccgtacaa cgtgtgtacg gctgcagcct gtacacgggc atacacttcc
      481 gcatccatac gacgaagcag cgtatcctgc tcaaagcggg ttaataaatc gctccggatc
      541 atagaaataa gttgcgggag gccggggcga taaaactgac tgtcagccat tcagttcact
      601 ccagatatca tcaaaaataa tgttgtgaat attgccgtca cgctggtaaa tggtaatggc
      661 aagtgccagc gagtctgtcc cggtccggac agcgttaata tcaagacggg aagcaacacc
      721 atcctccacc atccacgcca gcgcctcacg gacataatct ctggcaagct gcggggtttt
      781 atttgtcagc ttgctgcgtc gcaacagata caaacgcgac cccgtgcgat cattctgaac
      841 cgcaggccag gtatcccccc accatccaaa tatccgtggt gcatcatcac gcccggcacg
      901 ccgccatgta aaaagcgaaa taatcacagc acgcgtcaga aggtcgatcg aagccccggt
      961 cgacacgggt cgcccgttaa cattaatcat catgattttc agcccatcgg ttgatccggc
     1021 gtgtcagtga tcccgccacc atcaccattt tcggtatgtt tgtgggcatt gtaggcctgc
     1081 cgcatttgct gcatgctgag tccgccactg tcgcaattgt cagtaatatc ggcggtggat
     1141 tcgactggca tttcaaagcg cgctttaggc gcgtttttaa aaattatggc cttcccggcc
     1201 ccgtccacca caataccgga acgggtcagg acaacggact gtcccagatc gtcataaagc
     1261 gccacttccc cacgtttcag tcctttcagc cggtaacgtc tgtcagccac gaccacaacc
     1321 acaccatgag aacgatcgcc cgccggaaat aaagcaacgc cctcagcacc gttctgtgcg
     1381 gcagatgtaa aaccataagg ttcaaggtgc tcaacgtgtt gtttctgatc tccggctatc
     1441 attttcaacc ctacagactg acactttctg gcggaatcca ccgccgtgat gacagcccgg
     1501 gaaatcagat tacgaagaga aaacccgttc atcagaaatc ctcctcaaca tttttttctt
     1561 cctggcggta acaggctcgg ggagataagc atctgccggg ccaacccgta attcagtcgt
     1621 cgtgccccgg tcgtcctggt tataggtaac ttctgcgatc accagttcat cattatcaaa
     1681 attattcagc gggtcgaaaa cgatgacaga taaccccgga cgccataacg cgccactgcc
     1741 ctgccgccag ccctgaaccg tatatgttgt ctcacgggta agcgcagccc gttggcgcgc
     1801 ttcaaattca caacgggcct tacaggttgc tgtcgttgct gtgcctgact gctgaatcaa
     1861 caaagggcga taacgggtca cgccactgtc ctgaatggtc tgacgtattg cggcaatggt
     1921 ggcctcacca aaatcatcgt cgttgcccgg gcgctgccca ctgacctgat attcagaaaa
     1981 ccgctctctg atgcttcttt ccgtgtcaca ggaaagaata ttctctccca gcaccagcgc
     2041 cgtcgccgct ttacctgttc cgggttttcc cagaaccagt cgcccgcatt catcgtcata
     2101 agccagcgtc tgaacctgcc ccagtaaccg gttaagacaa tcggcaacgg tttcgccgtg
     2161 ttccggctgg gcatcaatca ccgctgtctg cggcacgcca gcatcaacaa cggtgatgcc
     2221 aaatggcgca gccagttcac tgactattct gagcaggttt tttccgctct gctggagtgg
     2281 caaagcagag cagtcaacca gatcggctgt tttgcttcgc ccgacaattc ccatgctgac
     2341 gctgctggcg tcataacgaa gcggtagtgc ctccacatat ccggtgagca caggctcatc
     2401 cccgataagc acttcaacca gctcaccatt ttttatccgg ggttgataat cccggcttcc
     2461 gggccagcgg gtggtaatgg caacattaaa atcccgggca atacggttaa tgcccgcact
     2521 gatacggacg gatgtccagc cgccccattc gcgaccggaa acccgaagta aaacggtatc
     2581 attcatctga cgggtaccct taatgccctg accggaacaa agcccggatg agagatggca
     2641 tttcgatcca ggatatcagt ttcacgggac gcatcgtcgt accatgacgc agccagaaca
     2701 agcgcaggca gaacttccgc tggcgttcgc tctgcggttt cctccgtctg aaccagacgc
     2761 gcctgaatat cccggttcag ttctgtacgt aatgacgtca gctgaaaaaa cagcctgtca
     2821 tccgccgtac gcctgagctc ctgttcaatc gccgcgttca gcgattcgcg tatgatggtg
     2881 agattttccc gcgtgggtgg ttgcgctctc tcatcctgtc ctgtgctggc tgccacgctg
     2941 tcaagtgccg gatgagaaac gtgaataata tcggactgac gttcagtgga accaccaaca
     3001 gccacaaccg cctgctgatt tttcaccagg cttccgggtt gtggcagtga agtgaccgcc
     3061 cttgtggctt cgctgattgc cgtcgtccgg atgacagcag ccacaaggtt tgtctgctgt
     3121 ttttgcctga taaccgatgc ggaatctgtg ggccacaccg cacgcggtgc cagtcctggg
     3181 tccagcgtaa tgccggacat tgtcgttatg gactgcacca gatcctgtgt gttatccacc
     3241 agccgggttc cggcccgcca ggtatcctgc aacgtatgca caaaatcact ggcaaccgac
     3301 ggcggcatca gaatgacgga taaatcgccc tgcagtagtc tcgctccggc agaaacagcc
     3361 gaattaacca ttttgaaagc tgtctgaacg gttcccagca tatcggtcgt ccgggcaata
     3421 acatcgttct gaataaagtc cggtattccc gcgagatcaa aatcgccgaa catgtcttca
     3481 atcaactcat ccaggaatac ggacgactcc tccagttttc tggcggttgc agctccggcg
     3541 accggaaatg acagttcccc gctctcaaca aactgaaatg aaacccggca catacggcct
     3601 tcagtgccgg agtgagaaac ggtcacctgc ccgtcaatac agccctgcat ttcaccgaac
     3661 tgcggatgga tcagcgtccc cggcccggcg gtttcaatcg ctgtaatcag cctgtcgcgc
     3721 tgctctgcgt aatcatcacc aacgagatac gcattaatcg tcagccgtcg cgtggcacgc
     3781 ccgagatctt ccgtgtacgg tttgtcacgg tttgggtatt catggacctg aacgcggcgt
     3841 ccgaacgaac cctcgtcgct ctccaccgaa aacggaacgc cacgaaatga agcgtcgtaa
     3901 agattatcgc gccaattgat acctgaagat gcagaaaaga gtgaagaaag agaaggtaaa
     3961 gaaggtaagt ccatactgtc tccactataa aaaaaccgcc aatttctggc ggcttatata
     4021 ttaatcatca ttaatatctt tcatattcaa tttgcaattt tcgtaggtca ctcccgactc
     4081 gccattaaca ttaactccag ctctgatatt cccgttttct tttacatcta caaaaaactg
     4141 gcctcttggc atcttgaacg caaatgtaaa accaacagta tctgaatcaa aatcatcatc
     4201 tttatatatt gggatcaaaa atgtcatcgt cgctggtgat gcattgtttg caaaatcaaa
     4261 tacacctata cttttgccga attcataaga agcttttctc aatataatcg caccgctggc
     4321 agaaggggat gggcacgata tcctcagttg tctgaatcca tctgtatcca agtcaacgtc
     4381 attatctgca gatgt
//
LOCUS       DAESHI010000206         4364 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.209, whole genome shotgun sequence.
ACCESSION   DAESHI010000206 DAESHI010000000
VERSION     DAESHI010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.209"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..215)
                     /gene="tuf"
                     /locus_tag="KW011_004214"
     CDS             complement(<1..215)
                     /gene="tuf"
                     /locus_tag="KW011_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBH7662393.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(330..405)
                     /locus_tag="KW011_004215"
     tRNA            complement(330..405)
                     /locus_tag="KW011_004215"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Thr,seq:ggt)
     gene            complement(412..486)
                     /locus_tag="KW011_004216"
     tRNA            complement(412..486)
                     /locus_tag="KW011_004216"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Gly,seq:tcc)
     gene            complement(603..687)
                     /locus_tag="KW011_004217"
     tRNA            complement(603..687)
                     /locus_tag="KW011_004217"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Tyr,seq:gta)
     gene            complement(696..771)
                     /locus_tag="KW011_004218"
     tRNA            complement(696..771)
                     /locus_tag="KW011_004218"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Thr,seq:tgt)
     gene            1133..2083
                     /gene="coaA"
                     /locus_tag="KW011_004219"
                     /gene_synonym="panK"
     CDS             1133..2083
                     /gene="coaA"
                     /locus_tag="KW011_004219"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBH7662394.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2112..3077)
                     /gene="birA"
                     /locus_tag="KW011_004220"
     CDS             complement(2112..3077)
                     /gene="birA"
                     /locus_tag="KW011_004220"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HBH7662395.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3074..4102)
                     /gene="murB"
                     /locus_tag="KW011_004221"
     CDS             complement(3074..4102)
                     /gene="murB"
                     /locus_tag="KW011_004221"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBH7662396.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            complement(4237..4352)
                     /gene="rrf"
                     /locus_tag="KW011_004222"
     rRNA            complement(4237..4352)
                     /gene="rrf"
                     /locus_tag="KW011_004222"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1112 a         1095 c          983 g         1174 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      241 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      301 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      361 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      421 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      481 gcccgcatcc tggaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      541 ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      601 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
      661 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
      721 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
      781 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
      841 tggtcacatt ttatgcgaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
      901 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
      961 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1021 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa ctgtgacgcg
     1081 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1141 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1201 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1261 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1321 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1381 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1441 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1501 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1561 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1621 caagtccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     1681 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     1741 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ctgattttgt
     1801 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     1861 ttttctgaaa ttccgcgaag gggcttttac cgacccggat tcctattttc ataactacgc
     1921 gaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     1981 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2041 aagtgctaat catgcggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2101 actccccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2161 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2221 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2281 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2341 aattcacgta ttagcatggc cgccaacgta ttacgatcga gattgatccc cgcttcctgc
     2401 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2461 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2521 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2581 gctcccagct tgcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     2641 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     2701 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     2761 gcatcgcccg atttaagctc tccgatacga tcaagaaggt actgattcgt ggagtcaata
     2821 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     2881 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     2941 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3001 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3061 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3121 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3181 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3241 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3301 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3361 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3421 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3481 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3541 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3601 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     3661 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     3721 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     3781 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     3841 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     3901 tgatccggtt gatgatcacc gtgccacgat agtcttccag aaaaagtaca ttacttcctt
     3961 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4021 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4081 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacaaggc tagtttaccg
     4141 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aacgcaaaaa
     4201 ggccatccgt caggatggcc ttctgcttaa tttgatgcct ggcagttccc tactctcgca
     4261 tggggagacc ccacactacc atcggcgcta cggcgtttca cttctgagtt cggcatgggg
     4321 tcaggtggga ccaccgcgct acggccgcca ggcaaattct gttt
//
LOCUS       DAESHI010000207         4330 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.210, whole genome shotgun sequence.
ACCESSION   DAESHI010000207 DAESHI010000000
VERSION     DAESHI010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.210"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..583)
                     /locus_tag="KW011_004223"
     CDS             complement(<1..583)
                     /locus_tag="KW011_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH7662397.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPNF"
     gene            complement(646..1095)
                     /locus_tag="KW011_004224"
     CDS             complement(646..1095)
                     /locus_tag="KW011_004224"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HBH7662398.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(1092..4184)
                     /gene="ebgA"
                     /locus_tag="KW011_004225"
                     /pseudo
     CDS             complement(1092..4184)
                     /gene="ebgA"
                     /locus_tag="KW011_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
BASE COUNT         1028 a         1090 c         1168 g         1044 t
ORIGIN      
        1 tgaagttcgg gatcatcgcg tcaacggtga tggcgtcagc aggctgtacg ccgccaacca
       61 gcgccgtacc tgccagtaaa atgtaggaga gcgttaacag cagcatcagc gttgaagtga
      121 cggaggtaat tggccccaac attttcgccc cgttggtgga aacccaggtg gagaaggcga
      181 acagcaccat actgataatg gtggtggcaa ccggcgtcat aatatattcg tatccgagga
      241 aggcatacga agcataagca ataacgcgcg gcaacagtga ggtgaaaaag aacaggttta
      301 cgaaccagta ggtataggca gtaataaatg cccaacgtcc gcccagcgaa ctttttaccc
      361 atgcgtagac accggcttct gagtttttat ttaacgaaac aaattctgcg atgatcagac
      421 agaagggaat aaaataaaaa atcgtcgcga ggaaaaacat cggtgccgag gccagtccaa
      481 gatcaatatt attattgata acgttgttaa agctgtaaac ggcggcaaaa gtcagcgaga
      541 gcaagccgaa tttgccgatt gtattacgtt tggtatcaga cataacacat ctccgaaaat
      601 gagtagggta gcggtggcgg caaactcctt ttgccgccgt agttgttatt tgttatggaa
      661 ataaccatct tcgatggtga ctttgagaac cacttttttg accgcgttat tgcagataaa
      721 ccgatacgct tcatggatat cgcaaataac gatttgccct tcgtggacct caacggtttc
      781 tccgcagcct tttaaatatt cacggtcagt ttcatcacga taatattcca ctacctgtaa
      841 tgtctctttc ggcgcatatt caattttttg ctgcccttgc aggtagtaat gcacttcaaa
      901 atagcgtcga tgcccggtaa atagcgcatc ggtcgcggag tctgtctcca cgcggtaagt
      961 caatgagtca ccgatggagt gggcgacgcc aggctgaatg ttgtcgatat tttcaatcgc
     1021 ttcaacgcag cgttgccact tcttgccaga ggcgtaaatc tggcggaact gttctaagtt
     1081 atcgatgatc ctcattgctt attctcgctg tgcaaattcg tggaaaagaa ccctgcgccg
     1141 aactcatacg acgccaggct ttgcgcggta gcttctccgc cagaaaccgg cagcaacgta
     1201 aagccgtagc tgaagtcacg gaaccagacg cgccaggagt ccagcacctc gctgccccag
     1261 gagttggagc cgaggccaag cagctggtga tcgaggttca gggtgatgtc atcactgcgc
     1321 tgcagctcgt tacagtgctg ggcagcgtgg atgttttcct aggtatagtg ccaggcgctg
     1381 aagttaattg ggcgctgcgg aaccaccagc agaccgttac cgtggcggtt agtcagtgcc
     1441 gtccagcgga catgctgacg gttaccgttg ttctgcggga agggatagtt ctcgaacatg
     1501 gcatcgacgg tgctgcgcca gatatcgatg atgttagcct gctggctgtc ggcgtagttt
     1561 tcgcccggtc cacgaccgta atacgccacc tgatcgtatt cgccgttaat tcccatggtg
     1621 aaaccgatgc acggaatgat gtgcggatag tcgccgtaac gctcgccgga aagcgccacg
     1681 ttaacctggc catcggcagc gatgcgccag atgtaggtgc agcgcattcc gaaatcaaac
     1741 accggcgggg caataaccgt gcggctgatg atcaacactt caccctcgct ctgttctacg
     1801 gcaaagtcgc gcagatgttc ctgcatgatc tgcaaatgat tcggctgcca caacccttcg
     1861 tactcctgct tgtggttgtc gatcatcggc ttgaagaagt tgatctttgg ctcgcgggtc
     1921 agcagcgact cgccatttac ctgccaggat gtcggtttgc cactcatttt tgagaaggtg
     1981 atcgcgaagt tgtagccgcg aacggtgcag ctcaaacgat cgtcttccag cgtcagcgga
     2041 cgcgcattat ttggtgcgaa aggcactggc tgcgcggtgt tttccttcag cgggaactga
     2101 taagtggcga tagaatgccc ggcttcgctg tagcgggtgc gggaatcttt ggtcaccgta
     2161 atgttgagga acgcttcgcg ggcgtccagc tgcggcagcg tgatctgcaa gggggcttcg
     2221 ctgttcggcg caacgtcgcg cagtttaatc tgctgcgtcg cgagcgtttc accttcggcg
     2281 cgcacctctg cgtgcagggt gtagtcatca agcgtggtaa accacagttt attttcgact
     2341 ttcaactcgc cgcgagtcag atcccgcgcg tggattttta ccggcgcgat aacctgtttg
     2401 tactctttca ggcccggtcc cggcgtctga tcggaataga tcaaaccatc aagacagaag
     2461 ttatagttgt tgggatagtc gccgtagtcg ccgccgaatt tataccagac attgccgtgg
     2521 tcgtcctgtg cctggatccc gtggtcgcac cactcccaga cataatgacc ctgaatgcaa
     2581 tcgtgcttat agaagacgtt ctggtactcc gtcagcccgc ccggtccgtt ccccatcgca
     2641 tgagcatatt cacagatgat gcgcggcttc ggatgcgggt attcaccaaa ctcattcatc
     2701 agcggcacgc gggtgtacat ggtggaaata atatcgacca cttcagcatc gcgatcttct
     2761 tcgtaatgca ccagtcgcgt gtcatccagc gccttcgctg cgtggtacat cgcgcggatg
     2821 ttacagccat agccggattc attgcccagc gaccagatga tgatcgacgg atggtttttc
     2881 tgcgcgtgaa tatggcgaac aatgcgctcg acgtagactt tttcccactg cggatcgtcg
     2941 gtaatacggc tgatatcgcc gacattagca aagccgtgcg attcgacgtc ggtttccgcc
     3001 atcacaaaca ggccgtagat atcacacagt tcgtaaaaac gcggatcgtt cgggtagtga
     3061 gcggtacgca cggagttgat gttgtgctgc ttcatcaact ggagatcttt ctcgacgcga
     3121 tccattccaa cggcgcggcc tttgcgatga tcgttgtcgt gacggttgac gccgtgcagc
     3181 atcacataac ggttattgat ccagaacaga ccgtcgcgca ctttgatatc acggaagcca
     3241 acgcgttgtg gtaccacttc cagaacgttg ccgttggcgt ctttcagcgt catgaccaga
     3301 tggtaaagat aaggggattc tgctgaccat tgctgcggct gttcgacagt aaaagcaaag
     3361 ctggcgctgg tcagtttttc aattgccaaa tgatcaatgg cgctgctgtg caccacgcgt
     3421 tcgccatcaa acagggtata ttccagcgtc gtgacgacag gggaggcggc gagattttcc
     3481 agcaccactt cgcaggaaag cgtggcatcg caataggctt cgtcaaagtc ggtacgcacg
     3541 gtgaaatcgt taatatgtgt tagttgtttt ccgatcagat aaacatcgcg gaagatcccc
     3601 gctgaccacc acatatcctg gtcttccacg taggtagagt ccgcccattg catcacgcgc
     3661 acacacaaca ggttgtcgcc ggttttaacc atcgcgctga tgtcaaactc tgcggtcagg
     3721 cgactgccct tgctgaaacc cacatactga ccgttaacgt agacttcaaa ataggtttcg
     3781 acgccgtcaa atttaatcag cgtctgttta ccctgccagc cgtcgctgag ggtgaaaata
     3841 cgttgatagg caccggttgg gttatcgctg ggaacaaacg gcacatcgat ggggaacgga
     3901 aaaccttcgt cggtatattg cagtttgccg tgaccttcca tttgccacat ggcggggacg
     3961 gtaatatgcc cccagtcagc cattaactca gaggtgaagg cttcaggcac ttgcagcggg
     4021 tggtcaaaaa agtggaaatt ccactgaccg cttaagggca gaaacaggct gctggtttcg
     4081 cgggcaaagg tacgcgcttg cgcaacagaa tcatatgaaa aaaagtacgc acgtggcgca
     4141 agtcggtttt cgtgggtgag ctgaatgttt tcccagcgat tcatagggcc tcccggtaga
     4201 gagaaatcgg aaccattcat ggtgatggta gtttgtgtaa ttgctgggga gtttagtaaa
     4261 atttttacta aaaagtggca ggtaaatgca tttactttaa ctggatcacg aaaataaacg
     4321 gcagcactgt
//
LOCUS       DAESHI010000208         4309 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.211, whole genome shotgun sequence.
ACCESSION   DAESHI010000208 DAESHI010000000
VERSION     DAESHI010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.211"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..1870)
                     /gene="speC"
                     /locus_tag="KW011_004226"
     CDS             complement(<1..1870)
                     /gene="speC"
                     /locus_tag="KW011_004226"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HBH7662399.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDA"
     gene            2269..2976
                     /locus_tag="KW011_004227"
     CDS             2269..2976
                     /locus_tag="KW011_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBH7662400.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3082..3157
                     /locus_tag="KW011_004228"
     tRNA            3082..3157
                     /locus_tag="KW011_004228"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3115..3117,aa:Phe,seq:gaa)
     gene            3355..>4309
                     /locus_tag="KW011_004229"
     CDS             3355..>4309
                     /locus_tag="KW011_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBH7662401.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSNKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         1114 a         1141 c         1037 g         1017 t
ORIGIN      
        1 gcgcatcctg ggggttcatc accactgacg ggaaactctg ctggcggaac atcgcttttt
       61 gtaggtcttt gacgtcgaaa ctgacataca gatcgtgcat ctcctgacac aactggcgca
      121 gggtgtagtc gcgatagcgc accggatatt tgttataaac gctcggcaac acctcagcca
      181 gcggcgagtc atcctcaata tgctgttcaa attgcgccag catcgccacc agttgtgcca
      241 gcttctcgtg gctttccgcc ggagttaata agaacagaat ggagttgaga tcgcacttct
      301 ccggcacaat gccgttctca cgcagatagt gcgccagaat cgtcgccgga acgccaaagt
      361 cgctatattc gccggtttcg gcattgatac ctggtgtagt gagtaacagc ttgcacggat
      421 caacaaaata ctgatccgcg gcatatcctt caaagccgtg ccacttcgcc cccggctcaa
      481 aactgaaaaa acggcggtcg ctggctaaca cagatgtcgg ataatcctgc cacaatttgc
      541 catcaacaac gggcgggata aacgggcgga acagcttaca gcgcgcaaga atagccttgc
      601 gcgactcaat acccaactca acgcactcag cccacagccg acgcccactc tccccttcat
      661 gaattttggc gttaacatcc agtgcagcaa acagcggata gaaagggctg gtagaagcat
      721 ggagcataaa ggcgttattc aaccgcttat gcgggcaaaa acgcgcctgt ccgcggatat
      781 ggttatcttt tttatggatc tgcgacgtct gtgagaatcc cgcctgctgt ttgtgcaccg
      841 actgagtcac aaagatcccc ggatcgtttt cgttaagttc taatagcagc ggcgagctat
      901 ccgccatcat cgggataaac tgttcgtaac cgacccacgc ggaatcaaac agaatgtaat
      961 cacacagatg cccaacggta tcgatcacct gacgggcgtt atagacagtt ccgtcatagg
     1021 ttcccagctg aataatcgcc aggcgaaacg ggcgcggcag ctcggctttt tctggcgcaa
     1081 cgtcgcgaat ttgctggcgc agatactctt cattaaaaca gtgcgcatca ataccgccaa
     1141 tgaaaccaaa cgggttgcgt gaagcttcca gatagaccga cgtcgccccc gcctgaatca
     1201 gcgcgccgtg atgattcgac ttatggttgt tacggtcgaa gagcaccaga tcgccacgcg
     1261 ttaacagcgc attcgtcacc actttattcg ctgccgatgt gccgttcagc acaaaatagg
     1321 ttttatcggc atgaaagact ttggctgcga atttctgcgc atccttcgcc gatccttcat
     1381 gaataagcag atcgcccaat tttacgtcag cgttacacat atcggcgcga aagacgttct
     1441 caccaaagaa atcgtaaaaa tggcgtccgg caggatgctt tttaaaaaac gcaccatgtt
     1501 gatgtccagg gcaagcaaag gtgctgttgc ccatctcaac gtactgcgtc agcgtgtcat
     1561 aaaacggtgg cagcaaattc tcttcatact gacaggctgc ggattccagc tccagccact
     1621 gctgctcgtt gccgttgatt accgccgtaa cgcccgcagg taattcaaca gcatgttcgg
     1681 aatacaaaaa caccggtaga tgaaaaccgg tgcgcttaag caacgcaaga atgccactgc
     1741 gactatccgc agcggtaatg acgactgccg cgacatccgt aaaatcagta tctcccaacg
     1801 ccaccacgcg gcgatgagta gaaagtcggg ataccagttc actactggcg gcaatattca
     1861 ttgatttcat aagcgcaaaa cccgtttcgg ggaagtaaga ataccggaca aagtgtaaaa
     1921 ccctgcccca tgagatatgg gtcaaactgg tcaccagctc cgaccgccag acatcagtaa
     1981 aagcagaaac gctctgattt tactgttgtc ctgcagtgaa cgtgcgttaa cttcaccgca
     2041 tgagcagtaa catagaaagg gaaacgtttc gcgcgaaacg gcgataagcg agagaatgta
     2101 aggagatggc gtgcatcggg caaactccgt acagagagga gaaaattcgc gcaatcatgg
     2161 cacctttcgc taaggcgtgc aagccaggac tttgcgacca gacaagccat caacaaggca
     2221 aaaagttcat aataagaaaa tcaaacaata cagctaacag gaactcttgt ggtcatcggc
     2281 ccttttatca acgctagtgc agtcttactg ggtggcgttc tcggcgcact gctcagccaa
     2341 cgcttaccgg aacgtatccg cgtctccatg acatctattt ttggtctggc atcgctgggg
     2401 attggtattt tactggttgt gaaatgcgcc aaccttccag cgatggtttt agctacgcta
     2461 cttggcgctc taattggcga aatttgtttg ctggagaaag gtgtcaatac agcagtcgcc
     2521 aaagcacaaa atctgtttcg tcactcacgt aagaagccag cgcatgaatc ttttattcag
     2581 aattatgtcg cgattattgt cctgttttgc gccagcggca ccgggatctt cggggcgatg
     2641 aacgaaggga tgaccggcga tccgagtatt ttaatcgcca agtcatttct tgatttcttt
     2701 acggcgatga tcttcgcctg ctcgctgggt attgcggtgt cggtaattag tatcccatta
     2761 ctgatcatcc agctaacgct agcgtgggct gccgcgctga tattaccgct gaccacaccg
     2821 tcgatgatgg cagacttcag cgccgtaggc ggtttattgc tgctggcaac cggattacgc
     2881 atctgtggca ttaaaatgtt cccggtggtc aacatgcttc cagcactcct tctggcaatg
     2941 ccgctttccg ccgcctggac cgcctggttt gcctgacaat gcgtgcaata gcggcaaagt
     3001 gatgatagat tgtgcagtct gcagtaaatt gaagaaattt gattgacgag acgaggcgaa
     3061 tcaggtttaa tgcgccccgt tgcccggata gctcagtcgg tagagcaggg gattgaaaat
     3121 ccccgtgtcc ttggttcgat tccgagtccg ggcaccaaat tcatatcaac ggacctccac
     3181 ggaggtccgt ttttcgtttc agaacgccac gatttaagcg ttctgcctac aaatcaattc
     3241 taccgaactc aaccagattc cccccacatc aaccccattg tgtgggtata attgcgggca
     3301 taccccagtt cgacagaatt tgtaccctct ttaacgcatt cagaaggctg aactatggca
     3361 ctgactgacg caaaaatccg ggctgcaaag cccactgaca aggcttataa actcactgac
     3421 ggggctggca tgttcctgct ggtacatcct aatggctccc gttactggcg tctccgttat
     3481 cgtattctgg gtaaggagaa gactctggca cttggtgtgt atccagaagt ttctctctcc
     3541 gaagctcgta caaaacggga tgaggcccga aaactgattt cggagggggt tgacccttgc
     3601 gaacagaaaa gagctaaaaa agtagtccct gatttacagc tctcttttga acatattgcg
     3661 cgacgctggc atgccagtaa taaacaatgg gcacaatcac acagcaataa agtactcaaa
     3721 agccttgaga ctcacgtttt cccctttatc ggcaaccggg atatcacaac actcaatacc
     3781 ccggatctgc ttatccctgt tcgtgctgca gaagctaaac aaatttatga aatcgccagt
     3841 cgtctgcagc aaagaatatc tgccgtaatg cgttatgccg tacagtctgg catcatcaga
     3901 tataatcctg ctctggatat ggctggcgca ttgactacgg taaaacgcca gcatcgcccc
     3961 gctcttgatc tttctcgcct gcctgaactt ttgtcgcgta ttggcagtta caaagggcaa
     4021 cctgtcaccc agcttgccgt tatgctgaat ttactggttt ttattcgttc cagtgaactc
     4081 agatacgccc gctggtctga aattgatatt gacaatgcca tgtggactat tccagccgaa
     4141 cgcgaacccc tgccaggcgt aaaattctca caccggggct ccaagatgcg aacaccacat
     4201 cttgtgccac tcagcaaaca ggctgtagcc atactgacag aactacagac atgggctggt
     4261 gaaaatggtc tgatctttac gggagcacat gacccgcgta aaccaatca
//
LOCUS       DAESHI010000209         4261 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.212, whole genome shotgun sequence.
ACCESSION   DAESHI010000209 DAESHI010000000
VERSION     DAESHI010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.212"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..3511)
                     /locus_tag="KW011_004230"
     CDS             complement(<1..3511)
                     /locus_tag="KW011_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH7662402.1"
                     /translation="MDENALGFTSYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQ"
     gene            complement(3963..>4261)
                     /locus_tag="KW011_004231"
     CDS             complement(3963..>4261)
                     /locus_tag="KW011_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBH7662403.1"
                     /translation="VNKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQM
                     SHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRDKGISPFEYAKINNPLK"
BASE COUNT         1098 a         1095 c          878 g         1190 t
ORIGIN      
        1 cctgacgcct ctcaagacta ttataaatag cctcaatctc acccttcagg ttatcaccgc
       61 aataaaccat tgatgtatcg tatcgttcat atcgcgcttc ccgtgtcgca ctaaaccagt
      121 tgaacgatcc cacacacagc aaaccatcat caccaataac aattttgcta tgaacacggt
      181 ttaccagctt tgtagcaata cccagcgcat tcagtttctc cagcgccgct ttaaagttct
      241 gctgcttctc ttttcgcttc tcaaaatcat tatgttcagt gttgtagctt ctgtcagtga
      301 ctatcgtgac gttaattcca cgtgaacacg ccgcaatcat ggaatcaaga aaaccggttt
      361 gctccagcct ttgccaggtc agccatggag aaattatcgt gatgtgttta ctggtatttt
      421 caaatgtctg attcaggaaa ttatcatgtt gctccacacc atgaagtgtg tagattttgg
      481 tcccggcggt ttttaaatcc ttacgctctt tataatcaaa agagagcgca ttcttctctg
      541 actcaaagag gtattttgcc agtaatcccc gtggcgatga ggctggctgg acctcaaaca
      601 ggtccatatc gccgaagacc agaaaactgt ccttcgcacg ggagactgca acattcagca
      661 tgctgttatc gctatcaata aacccgccgt cttcatgttt tgaatagact ggcgagaata
      721 tcacaatcgc tctttccgct ccctgaagag agtgcacggt gcccactgtg agcgactttt
      781 cattcgtgcc tgtactgata ccttgtttgc ccagcacctg tttgatagtg gatacctgag
      841 cgctaaaagg cgtcacaata ccgacaactt catgaagcga tttaccgtaa tgcgcttcaa
      901 tattttgctg gttctctgcc aaccagaccg ctatcgtttc agcctcaagc aaattatatc
      961 gacttccact acttgccagc tctcctttac catcaatatg gagatacccc attgcgggca
     1021 ttaaattgct ctcttcacgc cctcttttag gcaacaactt accgtgatag cagagcgtat
     1081 tacagtatcc aataatattg tcgtagcacc ggcggtgttc atataggtac ataccacgag
     1141 ccagttcggg atcatattga tagcgcgaag caaactgcgc tattttcata acgctgccag
     1201 atgcggcact tttaccaagg tctgcgattg ccgtatattt ctcggtaatc tcttcttgcg
     1261 tactgccaga cagaattttt tccgccagca tgttaccgac atcaatcgca ggagcaatac
     1321 tccatattgg cgggatctgc tccgtatcgc caatcactaa tgccttctta gctaatgcaa
     1381 acgaggcagc agccacttca ggaagcacct gcccggcttc atcgacaatg agtaaatcgg
     1441 caaaatcata caaataactt ttctcgaatt tacgttgtcc tttgtgctca cttatctgca
     1501 tattaccggg cagcatatag catgtcatta ccacacatgg cgtgagtttc attcgacgtt
     1561 gccaacgggc ggttacccct ttagcacctt ttttcttctt ctcgtcctgc agatcatcaa
     1621 tgctggccat atccatcagc caacgacctt cccagtagtg agtcgtcagt aaaaatgcag
     1681 ggaagcgaat ctgcgtatca gccagttcat cggcctgtga gaagctcagt tcctcgtcgc
     1741 cctcataccc taaatcaaat gccagcctct gccactcctg aaccgcctgc tgttctttaa
     1801 gaacgatttc atgggcggag tcaatctgct gccggtatgt tgtttgctcg cgctcagcgg
     1861 aattgagcag cccatcaata ttacgttcga tagtttcagg atcagaccac tgatttcctg
     1921 caatcagcgc gcctaattta tcttcgagaa acagctgtat ttggtactgt cgcttattgc
     1981 gaaccgccgg gagccaggaa aataatgaat agatcagtga ttcaccggcg cgatattttt
     2041 tccattccgt tttagcactc ttcagtagag tgactttttg ttcttgtccg gaaagtaatt
     2101 tatttaaatt atcgagatat tgctcaatat cattagcaat aagctcacgc gcagcccgaa
     2161 tctggcttaa cgtttgccat gttgcgttca gtcttatcag ttgctcagat tttgctgcca
     2221 actgaccatg caggagttca atgacctttt caggggatga acactctttt ccaggaaagg
     2281 ctgccttagc cttttccaga taaaacagca gtgcatcctc tacatactct tttgactcaa
     2341 cctggttgaa gaaatcttca gtttgatatt ttttggctgc ctcagcttta cgactgcttg
     2401 agggaaaata agcaccgaag cttttcagct ctggcaacca tcgcccggcc atcgcacctg
     2461 aaccttgcga aaagtctttc ccgaatgcct caataatgtt cgttacagcc tggttattcg
     2521 ttgaagtcgc gataataacc ggaggctcag atttttcgag agccgctctg gcccactgcg
     2581 tggcgatgat agaaagcacc agcgtggttt ttccggttcc cggagggcca ttaacagcaa
     2641 ggatatcgcc atgtcttgca tccagaaaat ggcttaaggc atcgcgctga gcctttgcca
     2701 gcggaaactt atctccggag tgtccaagcc tgtcgctgaa ttttgctcct ggggccagca
     2761 aagactctgc agcatgaacc tctcgcgagg cgaagcgatt gaagagcggc acatccttat
     2821 tgcaaacaat caggtgatca taaagagaaa ggatatggga actggcaccg ccagattgag
     2881 ccgttttaac aatataaccg tgctcagcga gttcatattg ctcaggtttt tcaatccagt
     2941 cgccggcaac gctcttcagt agcctctctg agtcatacag atattgacgc cactcctgct
     3001 gcaaggcggc atatcgtgct tcccgttctt catccgtttc ggcagtctta tcaacgctgt
     3061 catcaaagtt gatagagaac gtggtatggg tcgtcttgta tttgtcatac tgcccaatct
     3121 caccaatcga aaatgctcct tttggcaaag gttcaagcag gtctctggga atggaggtcg
     3181 ctggcgtcgg atataaaaaa ccctcacggc ttagcaaggc tggcgtcact atcggggtaa
     3241 caatatcagg cgcacctgca gaacggtcct taccatgctg cagtaaccgg aaataaactt
     3301 ttgggcgcaa gatgacatcg accgtttcga catcgtcttt ttctccctta aaaaatttac
     3361 tgacaatcgc ttcgtcaaga cgtcccgccg ctatcccatg ccagtgagtg aaatttttgg
     3421 cgtctttccg ttcaaaactg ccctttcctg actcagcatc cgcaagcgag ttgcgccagt
     3481 atgaggtaaa ccctaaagca ttttcatcca ttaccagtcc tgtgactcaa tgaaatccat
     3541 tgatttatcg aatataaaac caccatatgg acaactattg tccatattga aaaagttgga
     3601 tgcaatgttt tgtttataat tcggtatcaa atgtactggt tgtacaaggt tcagaacaca
     3661 agcacattca acagtacgtc aggactattg atttcataac agtgaataca ttttttaccg
     3721 tttataatga ctgcctgtgt tggacaagga taccagaaca gccgaaaaca ccgggggtta
     3781 ttacctgctc tcatggaaac cagatattcc tggtgctaat cacagtcata aatagcctga
     3841 agaacatgac gaagggactt taatggaagc cggagtaact tgccagaaca tcaatcttcg
     3901 tcatcattta caaagaaaaa taatcagtta aagcagtccg ggcaatcagc ccggactgct
     3961 tactatttta atggattgtt aatctttgca tattcaaacg ggctgatacc cttatctctg
     4021 ttcgcatcca gaaaatctgc ccaccactgt agcatcagtc gacgttcttc cagatgttct
     4081 gctttatgga tatacgcagc acgtacacca ttacgctcct gatggctcat ctgacgttcc
     4141 acagcatcgc gggaccacaa acctgattca atcaatgcac tgcacgccat cgcccggaat
     4201 ccatggccac agacatcctg ggttgtgtca taccccatca ccctcagggc tttatttaca
     4261 g
//
LOCUS       DAESHI010000210         4198 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.213, whole genome shotgun sequence.
ACCESSION   DAESHI010000210 DAESHI010000000
VERSION     DAESHI010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.213"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..1361)
                     /locus_tag="KW011_004232"
     CDS             complement(<1..1361)
                     /locus_tag="KW011_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HBH7662404.1"
                     /translation="MNRTSPHYCRRSVLSLLISALIYAPPVMAAFTTNVIGVVNDETV
                     DGNQRVDERGTTNNTHIINHGQQSVYGGVSNGSLIESGGYQDVGRNNNYMGQSNNTTI
                     NGGRQTIHDGGISTGTIIDSGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGGQ
                     TVTTQGHVDGTTINKSGYQDITQGSMATNTIINGGRQYVEQSTVGTTTIKNGGEQRVY
                     ESHALDTTIEGGTQSLNNKSTAKNTQIYSGGTQIVDYTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVTRNATNTIINGGTQNINNHGIATGTNIN
                     SGTQNIKSGGKADTTNISTGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALV"
     gene            complement(1426..1545)
                     /locus_tag="KW011_004233"
     CDS             complement(1426..1545)
                     /locus_tag="KW011_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBH7662405.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1700..1918)
                     /locus_tag="KW011_004234"
     CDS             complement(1700..1918)
                     /locus_tag="KW011_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH7662406.1"
                     /translation="MKKLLRTLSAVDARVVCKPHRLGRSMWHLVVLLEELCKRGINFR
                     ALAQSIFAQQWGDECCKSKRICDLKVIV"
     gene            complement(2272..3750)
                     /locus_tag="KW011_004235"
     CDS             complement(2272..3750)
                     /locus_tag="KW011_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HBH7662407.1"
                     /translation="MLVSKSNEFNTSSFIDSGNCNERKSSESMELLAYSIINLICKEA
                     ASGTYRGALETLQKMMSECIYHEGNAFVIMGSGEQLKRIKYDVDENNLKVFNVYFDNN
                     EELVTDGEPDVVCLSKQVWEDLLIKLKLENKENAVSETDLSSNKNNVDQFIECAKRNE
                     QTLFDNIRKSDFHVDSFKPGSTRSVILETQPNVCMEPHNLYDNQIDKVSPVTKNSQQV
                     KGHYGDKFKEMQMFLNQMSNALQQTPSLSESKEHTIDIQKITDAFVKEFRGVLIDKNG
                     NCSERLFNFYECCYKFLPRAQPQDKIESYNSALQAFSIFRSSTLNNNDVGFNFKLFPE
                     VKLSGENLETVFKYKKGSFVREIARINITLQKEEDGLYNLGGLDFKGCFFSGQNFSNY
                     DIQYVNWGTSLFDLDTPCIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGV
                     APFDDILFMDDDFDDSSSEDDPVENSPVVTSPVVSSSKSSFQ"
BASE COUNT         1185 a          920 c          738 g         1355 t
ORIGIN      
        1 actaaagcac tgcccgtctc ctggttaacc ccatgtgcta taccgccggt atagacaatc
       61 agcgagcctc cggcgctaat attgctgcca gttgccgtac catctttctc aacaacctgc
      121 cggctcccgg tggaaatatt tgtcgtgtca gctttcccgc cgctcttgat attttgtgtt
      181 ccgctgttga tattggtacc cgtggcaata ccatgattat taatattctg tgtaccacca
      241 ttgattatgg tattggtcgc gtttctggta acatccatta ccccgccgtt atctattcgg
      301 gtcgcatcag ctttagcatt cgttaaaact gacattgttc ctttatcttt aataatcgtc
      361 ttatttgccg aaccatatgc gtttatgtct aaatgaccac cgttttccag caacacattg
      421 tctgccacgt gattgtggat ggagaatgca ccttcactat tcgtaccgct caccgtcgta
      481 ccgttagtgt ttgtttttaa aattgcacca tcgtgctggg taacatttgt tgccgtacca
      541 ccactaacat caagcacgcc accggaataa acttcaataa catccgagga gctggtgtaa
      601 tcaacaattt gcgtaccacc agaatagatc tgagtatttt ttgccgttga cttattattc
      661 agagactgag ttccaccttc aatcgtcgtg tccagcgcat ggctttcata tactctttgc
      721 tcaccgccat ttttaatggt ggttgttcct actgtgctct gttcaacata ctgtcgtcca
      781 ccatttatga ttgtgttcgt tgccatactt ccttgcgtga tgtcctgata accggactta
      841 tttatcgttg taccatcaac atgcccctga gttgttactg tctgccctcc accatcaatg
      901 atggttccat tcgcagtccc cccacttatg tgtgaattac cacccttaat tgtcgttcca
      961 ttgctgatac cacctgtata aacgtcctga ttgccactgt cgattatcgt accggtggaa
     1021 atgcccccgt catgaattgt ctgtcttcct ccgttaatgg ttgtattatt agactgcccc
     1081 atataattgt tattccttcc aacatcttga tatccaccag attcaataag acttccatta
     1141 gatactccgc cataaacact ctgctggcca tggttgataa tatgagtgtt atttgttgta
     1201 cctcgttcat ctactcgttg gttgccatct acagtctcat cgtttaccac accaataaca
     1261 ttagtagtaa aggccgccat cacgggcggg gcatatatca aggcagatat caataaggaa
     1321 agtactgagc ggcgacaata atggggactg gtcctgttca taaatttcat cctctgaaaa
     1381 gtgaatactg agtagcgttt aagcgacttt agctctgctg caacatcagc ccgcaggcac
     1441 cagaccgggg ggttcatcct gaagagacag cgcaagtgta ttgtgttcac cgctcatcaa
     1501 agacatcatg atgaaatgat gatgttccac ataagaaaga ggcatttttt aaacacagtg
     1561 cgctgaagtg tgattggata aaaaagtcaa tctattcagg aaatacgggc gtattctttt
     1621 ctttcgacaa tcaggccgtc ggcaaaataa aatgatttac ataatcgttt ctgatgaata
     1681 tcttctgctc acataaaaat cacacaataa ctttgagatc gcagattctt ttacttttac
     1741 agcattcgtc cccccattgt tgggcaaata tagattgggc cagagcacga aagttaatac
     1801 cacgtttgca cagctcctcc aacagcacga caagatgcca catactgcgc cccagtcggt
     1861 gcggtttaca gactacccgt gcgtccaccg ccgataatgt cctgagcagt tttttcagtc
     1921 cggacctcct gccacggttt tacgttgaag aggtaaccct gagcacgcag ttcttcagtc
     1981 aggcgtggtg caccgtaacg ctgttattgc tgggtaagat caaaaaactt tcaggcagct
     2041 aaggaaagtt gaaccagaca ttagatgaaa tatttcaacc aattacagca ccaatttaaa
     2101 ctcagtaaat cacagcacag atcaaggggt tacgtgaaag cgtagcccct ttttctttgg
     2161 tagtggcagc aaaatggttg tagtgtaaaa aataatcccg tttaatcaat caataataca
     2221 tattgtttca atctacgtta ttatctcttt gtaaaaatag ccatttatta atcattgaaa
     2281 actgctttta gaacttgata caacgggact agtcacaaca ggactattct caacgggatc
     2341 atcctcagag gaactatcat caaagtcatc atccataaat aaaatatcat cgaatggtgc
     2401 cacgcccgtg atgagtttta ttttattatt acgatcagtc aagactccac ttaaaccgtt
     2461 ttcgctcaca ggttttaatg atttttcatt actcttgttg taagcaggcg cattaaaaat
     2521 acacggagta tcaagatcaa acaatgacgt tccccagttc acatattgaa tatcatagtt
     2581 actgaagttc tgtccagaaa agaagcatcc cttaaaatcc aatccaccta aattatataa
     2641 accatcctct tctttttgga gagtaatgtt aattctggct atctcccgga caaaacttcc
     2701 ttttttgtat ttgaataccg tttcaagatt ttcaccagac agcttgactt ctgggaataa
     2761 tttaaaatta aaccctacat cattattatt caaagtagat gaacgaaaga tggaaaaagc
     2821 ttgtagtgct gaattatagc tttcgatttt atcttgaggc tgcgctcttg gtaaaaactt
     2881 atagcagcac tcataaaaat tgaataaacg ttctgaacaa ttcccatttt tatcaattaa
     2941 taccccccgg aattctttca caaaggcatc cgttattttt tgaatatcta tggtgtgttc
     3001 ctttgactct gacaaagatg gagtctgttg aagtgcattg ctcatctggt taaggaacat
     3061 ctgcatttct ttaaatttat ctccatagtg gcctttaact tgctgagagt ttttagttac
     3121 aggtgaaacc ttatctattt ggttgtcata taaattatgt ggttccatac agacatttgg
     3181 ttgtgtttct aaaataacac tacgtgtact acctggctta aatgaatcaa catgaaaatc
     3241 actttttctt atattatcga atagggtctg ttcatttctc ttagcgcatt caataaactg
     3301 atcaacatta tttttattcg atgacaggtc agtttcagaa accgcatttt ctttgttctc
     3361 cagctttagt ttaatgagaa gatcttccca gacctgcttg cttaaacata ctacgtcagg
     3421 ctcaccatca gtaactaact cttcattatt atcaaagtat acgttgaata cttttaagtt
     3481 attttcatca acatcatatt taatacgttt taattgctct ccagatccca taatgacaaa
     3541 ggcgttgcct tcatgatata tacattcaga catcattttt tgtaaagttt caagagcacc
     3601 gcgatacgtc cctgaggcag cttccttaca aattaaattt ataatactat aagctagtag
     3661 ctccatggat tcagaagatt ttctttcatt acaatttcca ctatctataa atgagctagt
     3721 gttaaattcg ttgcttttac taactaacat tgtctattcc tcagttaatg tctacatggc
     3781 tatttttaat tttattattg tttgtcacta taaaaaatcg ctcatttgag acaattgctg
     3841 gcattaacag cttcatatgc tatacatggt acttttaatt aaattagcac aagaatgtta
     3901 aatttaataa acaaaaagtt atttcgctgt atgataaaaa ccacccgtta taatttatta
     3961 gtgaaattcg tttttcgagt gttagaaatt tatatctaaa tagcgttgat taatgagcaa
     4021 agccagactc ctgtaacgct cacaagactc atctacctgc gacgggtatt ctggtcagta
     4081 gtagatgttt aaggcgtggc agagacattt catccttact ctacggcatt ggcccgcata
     4141 caaatcgtgg ttgtgacact cacttatcat cagtgagcga acagagaata gttcagtg
//
LOCUS       DAESHI010000211         4179 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.214, whole genome shotgun sequence.
ACCESSION   DAESHI010000211 DAESHI010000000
VERSION     DAESHI010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.214"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..1358
                     /gene="dcuC"
                     /locus_tag="KW011_004236"
     CDS             <1..1358
                     /gene="dcuC"
                     /locus_tag="KW011_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBH7662408.1"
                     /translation="VVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIMGHKVLPSSQ
                     ASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQY
                     INSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGAAAAICASPA
                     AIILAPTSGDVVLAAQASEVSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEHI
                     SHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVICMLIASILEF
                     LRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSFG
                     SASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQASN
                     LGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVTG
                     K"
     gene            complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="KW011_004237"
                     /gene_synonym="citB"
     CDS             complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="KW011_004237"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HBH7662409.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2048..3706)
                     /gene="dpiB"
                     /locus_tag="KW011_004238"
     CDS             complement(2048..3706)
                     /gene="dpiB"
                     /locus_tag="KW011_004238"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HBH7662410.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHLNPEKIGYPMQFTKQGALEKGESYFITGKGAMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLNVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT         1030 a         1062 c          965 g         1122 t
ORIGIN      
        1 gggttgtggt tattgtgggt gtagctcgct acatcattaa agggtattcc gccactggtg
       61 tgttatttgt cggtggcctg ttattgctga ttatcagtgc cattatgggg cacaaagtgt
      121 taccgtccag ccaggcttca acaggctaca gcgccacgga tatcgttgaa tacgttaaaa
      181 tattactaat gagccgcggc ggcgacctcg gcatgatgat tatgatgctg tgtggatttg
      241 ccgcttacat gacccatatc ggcgcgaatg atatggtggt caagctggcg tcaaaaccat
      301 tgcagtatat taactcccct tacctgctga tgattgccgc ctattttgtc gcctgtctga
      361 tgtctctggc cgtctcttcc gcaaccggtc tgggtgtttt gctgatggca accctatttc
      421 cggtgatggt aaacgttggt atcagtcgtg gcgcagctgc tgccatttgt gcctccccgg
      481 cggcgattat tctcgcaccg acttcagggg atgtggtgct ggcggcgcaa gcttccgaag
      541 tgtcgctgat tgacttcgcc ttcaaaacga cgctgcctat ctcaattgct gcaattatcg
      601 gcatggcgat cgcccacttc ttctggcaac gttatctgga taaaaaagag cacatctctc
      661 atgaaatgtt agatgtcagt gaaatcacca ccactgctcc tgcgttttat gccattttgc
      721 cgttcacgcc gatcatcggt gtactgattt ttgacggtaa atggggtccg caattacaca
      781 tcatcactat tctggtgatt tgtatgctga ttgcctccat tctggagttc ctccgcagct
      841 ttaataccca gaaagttttc tctggtctgg aagtggctta tcgcgggatg gcagatgcgt
      901 ttgctaacgt ggtgatgctg ctggttgccg ctggggtatt cgctcagggg cttagcacca
      961 tcggctttat tcaaagtctg atttctatcg ctacctcgtt tggttcggcg agtatcatcc
     1021 tgatgctggt attggtgatt ctgacaatgc tggcggcagt cacgaccggt tcaggcaatg
     1081 cgccgtttta tgcgtttgtt gagatgatcc cgaaactggc gcactcttcc ggcattaacc
     1141 cggcgtattt gactatcccg atgctgcagg cgtcaaacct gggtcgtacc ctatcacccg
     1201 tttctggcgt agtcgttgcg gttgccggga tggcgaaaat ctcaccattt gaagtcgtaa
     1261 aacgcacctc ggtgccggtg cttgttggtt tggtgattgt tatcgttgct acagagctga
     1321 tggtgccagg aacggcagca gcggtcacag gcaagtaaat agtaatgccg gaggcaagtt
     1381 tcctccggct ttatcatgtc acccactgtg gtatatgcgt tgtggtctgc caactttgcc
     1441 gtgaacaatt tcagcaataa tcagatggcg gctggcgcaa tattcaagat aacgcctggc
     1501 agtggtgcag ctgatggtca gtgcctgcgc caccgtttct gccgtatgtt gcacaccagg
     1561 ctctttaaac agttttcgca ccgcgtttag cgtcaagggg tcaatgccgg tcggtagctc
     1621 gtccttaggt tcaccgcgag cataagcatt aaacatctca tcaatttgct tctggctggc
     1681 gctatcaata ctttccagca tatgtttacg ctggcggaaa cgggttagcg tttgccccag
     1741 ccgttcatag gcaatgggct taatgagata atcaaataca ccacaacgta cggcttcaga
     1801 caccgtttcc atatcgctgg ctgcagtggt aaacaccacg tcgccgggat aatgcgcctg
     1861 caccagttca tgcagtaaat taatccctct accgtcagga agatagttat cgagcaagat
     1921 tagccccggc ttaaaacgct cgatcatcat tcgggcctgc gccagatttc ccgccagtaa
     1981 tatctgactg aatccgggaa tgtgacgaat atattccgca tgcatctctg ccagcggcgt
     2041 ttcgtcctca acgatcaata gggttaatgg agctgtcatt aggtttcact ttcggaatat
     2101 agattgaaaa taaggtaccg cagggatcat tatcttcgag agtgataaca ccaccgcagc
     2161 gcgttacgta gctggcaatc aagtacaacc caatgccatg ttcaccgggc tcgtcagcac
     2221 gcgtactgac cccctgctca aatattttgt ctcttagaga ctctggaacg ccgcagccct
     2281 gatcggcgac ttcaatcacc acatcatcgc cttcatcgct gaggaataat tcaacgatct
     2341 tgtttccttc atcgctacgc aggctggctt cgaaggcgtt atcaagtaaa ttgccaacaa
     2401 tggcggcaaa ctcggtgcta tccagtcctg gcggcagttg cgaaagctgg ctaccgggga
     2461 cgatgatcat ttttagcccc agttcgcggg cgcgctgcac tttaccaaaa agcagccccg
     2521 ccacctggcg atcggcaaac gcctcacgca ggctgtcaat aagctgttgc tgggcctgag
     2581 actccccctg caccatggcc agcacgcgat catactcttt catctgcaac agaccattga
     2641 gcgtcgacat ccaattgaga tgctcgtgtc gcaatgtacg aaggctctcg acgtattgtt
     2701 ttatttgcgt cagttgcgca ttgagggtgg aaatttcgtc tttactacga aagctgataa
     2761 tggcccccag caaatcatca cctgaacgaa tagcttcccg gttggcaata acgttcagac
     2821 cattaaagtt cgccaccaca tcctgacgtt tttcatcaat ctgttcggta aagaaatcgg
     2881 cgggcctgac cacttcagca atgggtttac ccaaccattg ccgtccggga gagctcagcc
     2941 ccagcatctt tcttgcgtta cgattgatgg cggtaatgta accatgcgga tccaccgcaa
     3001 tcagcccttc ataaaccgaa ctaaacagcg cctcttgctg acggaccacg cgtgcgattt
     3061 gctttggctc catgcccatc atttgtcgac ggatatgcgc ggccaggaac cacgacagca
     3121 acatcagaat ccctaacagc acgacaaaca cgcctgccat cggtaataaa aactcagccc
     3181 gccagctatc gattttactc accaggtagc caatcgacac cacgccgatg acttttccat
     3241 cgtcatcaaa gattggcgtt ttggcgcgca tcgccatccc cattgcccct ttcccggtaa
     3301 tgaagtagct ctcccctttc tccagcgcgc cctgtttggt gaactgcata ggataaccaa
     3361 ttttctccgg attaagatgg taaaggcgga tcgagtgccg gtcgccaatc accacataat
     3421 caaaatcggt atctctttgt aatttgttag cgatggtcgc cagccgtttg tagtcacgcg
     3481 ttttcaccgc agagatgaca ctgtcattgg aggcaataat tttcgcctga ttcattgcca
     3541 tgtcgcgtac atgaagcgtt aaatagtcct caaaactggc cgtaaaatat tgcgccaggg
     3601 ctgcaatgac aaatattgag aacaccagaa tcagcaaaaa gatacgcagc ggaaatgcca
     3661 gtctttggaa aaatgcaaac tgtttattct cgttaagctg caacattgtt ttccttgcgg
     3721 gttgtcccga gcctctttga cggatgtaat taaaatgttg aatcaaaata catagatatt
     3781 aattatgatc gtggcaaatt ttattcatta aatcaattaa atcacttaaa cctgcaggtc
     3841 atttcgtaat tacattaatt aaaaaactta aaaccattaa ataaataaaa aaccactaac
     3901 tctatgtgaa ataaatcaaa atttcacgct gcaatactcc ttagaatgta tagcgaaagg
     3961 agaaaaagat atacctcgat cacccccctt tctcccaagt gaaaataaaa ggttatcaac
     4021 ttgcaacatt gaacaacatt cgttgcaaat cgataacaac atgcaccttc aggatactat
     4081 ttattatgtt cggcaatgat attttcaccc gcgtaaaacg ttcagaaaat aaaaaaatgg
     4141 cggaaatcgc ccaattcctg catgaaaatg atttgagcg
//
LOCUS       DAESHI010000212         4169 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.215, whole genome shotgun sequence.
ACCESSION   DAESHI010000212 DAESHI010000000
VERSION     DAESHI010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.215"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            172..891
                     /locus_tag="KW011_004239"
     CDS             172..891
                     /locus_tag="KW011_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vimentin yjdA"
                     /protein_id="HBH7662411.1"
                     /translation="MKFSDRREALKLMTQIESTVTSLHREAEAQFRPELEKIVRGIET
                     GFRGTALYATENIAGRINARLEDEGFTVKITFPAVSQLQTRIAVKTNLSALMEERTET
                     VTRRRRQSGFWGKICGAFGTSDWGWENYKENVSRSVININSVRKEVMSLTRAYFGELQ
                     ASIEQNINQPVRQEIDDFFCTFREKVEQLRNTLIQSSEDHKRDQQAQEQLTERLQALN
                     ERVPELITDSKALREELETLL"
     gene            888..1793
                     /locus_tag="KW011_004240"
     CDS             888..1793
                     /locus_tag="KW011_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HBH7662412.1"
                     /translation="MTSPFIQQIADNRVCQVLSCLPEKFVVDFANGIDVAQEHNRTAG
                     GRTFFRRLKEGLTGKGAVRQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLAEQVHQKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     NGGSATVRLNTLDWLGGQSREQADNEWHEAVNWLGDWCSEERHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGEA"
     gene            1790..2860
                     /locus_tag="KW011_004241"
     CDS             1790..2860
                     /locus_tag="KW011_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBH7662413.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGAQISMVSGASIG
                     ALNGAIITASPDLSEAALRLEALWDHLGNNQVLSVNRSVYFSLLKKLVQAMNLCQIPG
                     RAGALLTTLFRHISTINGFDNPMIQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMYGDGGMGGWRNRQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRRAYPDTTILEIRPR
                     KRLKQIGDEGKSGGLLSFTSAHTDAWRQQGYEDTMLTMEHIRKPLAARQALTRSETVL
                     QKSLEITEGADSALRNAMARIK"
     gene            2992..3450
                     /locus_tag="KW011_004242"
                     /pseudo
     CDS             2992..3450
                     /locus_tag="KW011_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771527.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3576..4115
                     /locus_tag="KW011_004243"
     CDS             3576..4115
                     /locus_tag="KW011_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HBH7662414.1"
                     /translation="MNWHYEKNGVRYDNVTEEDIIERIRRGELNASTLVWQQGMLEWQ
                     PVSATSLAEILKQSEVPPALPGHRIPGGVVWTLAFAPLIGYILEMWTAGLNGMSFERA
                     YDAVSEGQYWFISLILNIALGYLDERKLRKSGVDTATFGWLAWLIPVYLWRRAKILGQ
                     KPAYFWVWLVMLILVLFTV"
BASE COUNT         1100 a          924 c         1123 g         1022 t
ORIGIN      
        1 gctttgaact ctttagagta acgttgggtt tttctgctca ttattagctc cttctgatgc
       61 cattctattt caggaaggag tgtccgttaa actcaggcta cctcacagtt tagtgtcagt
      121 gccccgggcc atcattcatg gaaacgagac tttgatccgg acagccccga gataaaattc
      181 agtgatcgca gggaagccct taaactgatg acgcaaatcg aatcgaccgt gaccagcctg
      241 caccgtgagg ctgaagcaca gttccggcct gaactggaaa aaatcgtcag gggtatcgaa
      301 acaggttttc gtggtacggc cctgtatgcc acagaaaata ttgccggacg tatcaatgcc
      361 cgcctggagg acgagggctt cactgtaaaa atcactttcc cggcagtcag ccagttacag
      421 accaggatcg cggtaaaaac aaatctgagt gcgcttatgg aggaaagaac tgagacagtc
      481 acccgtcgcc gtcggcagag tggtttctgg ggaaagattt gtggagcgtt tggcaccagt
      541 gactggggct gggaaaacta taaggagaat gtgagccgca gtgtgatcaa tatcaactcg
      601 gtcaggaagg aagtgatgtc actgacccgg gcatatttcg gggagttgca ggcatccatt
      661 gagcagaata ttaaccagcc cgtccgccag gagatcgatg attttttctg tacattcagg
      721 gaaaaagtcg aacagctacg taacacactc attcagagct ctgaagatca taaacgcgat
      781 cagcaggcac aggagcagct tacggagcgc cttcaggcat taaatgaaag ggttcccgag
      841 ctgattactg acagtaaggc gttaagggaa gaactggaga cactgctgtg acctcaccat
      901 ttattcaaca aattgctgat aaccgggtat gtcaggtact cagttgcctt cctgaaaaat
      961 ttgtggttga ttttgcaaac ggtattgatg ttgcacagga gcataaccgc acggccgggg
     1021 ggcgcacgtt tttccggcgt ttaaaagaag gtctgactgg caaaggcgct gtacgccaga
     1081 atgccatcaa tgcttcgctt gcacagggcg ttgaggcgtc cctgcgctgg ctgacggagc
     1141 tgacaacgtc gcttgccacc acaaattacg cgattacccg ggtaaacgac agggtcagtt
     1201 cactggtcag tgatactgcc aggctggcgc attattcggc agacacgcgg gagcagttac
     1261 tcaccctggc agaacaggtt catcagaaac tgaatcatct tgaagaaaaa ctccaccgtg
     1321 ttgaccaggt tcagcgggca cagttacatc ttgagcagat attctcatgg tggagcgccg
     1381 ggcgatacgc gtcattttcc cctgccggac gttgttatgt ggcgcttgaa gagcttcgct
     1441 ggggcgcgtt tggtgatgtg atacgtcagg gtgaaacagg ccaggttaac cagttactgg
     1501 atatcctcag acataaagca ttaacgcaaa tggcacagga gaatggcggt agcgcaacag
     1561 tacgtctgaa cacactggac tggcttggtg gtcagagccg ggaacaggct gataacgaat
     1621 ggcatgaagc ggttaactgg ctgggggact ggtgcagtga agagcggcat cctgtgatct
     1681 ggtcaaccac acaggctgca gaacacttac cggttcgtat gccccgtctc tgctctgcag
     1741 aacgcctctc tgaaagtatg gttgatgaaa tatttcagaa gggggaggca tgagtacaga
     1801 aatgaaaacg gggctggtgc tgtccggcgg cggggcggtg ggcgcttatc aggcgggagt
     1861 ggttaaggca ctggcagagt gcggcgcaca gatcagtatg gtctcaggag ccagtatcgg
     1921 ggctctcaat ggtgccatta tcacggcctc tcccgatctg tcagaagccg ccttacgcct
     1981 ggaggcgctc tgggatcatc tggggaataa tcaggttctg tcggtaaaca gatcggttta
     2041 cttttcattg ctgaaaaaac tggttcaggc catgaacctc tgccagatcc ccggacgtgc
     2101 gggcgcactg cttacgacgc tttttcgcca tatatcgaca atcaacgggt tcgacaatcc
     2161 gatgattcag ccgctgttgt cagatgagcc cctgacagcg ctgatggatc attatcttga
     2221 tacagatgct ctggccgacg ggctaccgct gtacgtgtcg ctgtacccca cagaaggagg
     2281 catgcaggat attattgact gcattcgtgc tgaactgggt gccggaacca cgaaaaacgc
     2341 tgtttttcag catatccaga gtctgccccg cgggcagcag aaagaagcct tactggcgtc
     2401 agccgcgctg cctctgctgt tccgtccccg cgaggttcag ggaacaatgt acggtgatgg
     2461 tgggatgggc ggatggcgaa acaggcaggg gaatactccc gtgacgccac tggtggatgc
     2521 cggatgcaat atggtgattg tgacgcatct gagtgacggt tctttatggg atcgtcgggc
     2581 ttatccggac accacaatcc ttgaaatccg tccccggaaa aggttgaaac aaatcgggga
     2641 cgaaggcaaa agtggcggtc tgctcagttt tacatcggca cataccgacg cctggcgtca
     2701 gcagggctac gaggacacga tgctgacgat ggagcatatc cggaaaccgc tggcagcacg
     2761 tcaggcactg acccggtcag agacggtatt gcagaaaagc ctggagataa ctgaaggtgc
     2821 agattcggca ctgagaaacg cgatggcccg gattaaataa agacgctccg gagaaacgcc
     2881 accgccactg tggtggtgtt tttcctgtct ggtattattg aaaatatttt cagatatata
     2941 ttacctggat gatggttaag attaatttat ttaattttgg ggagaaaact catgaaactg
     3001 ttaaacattc ttttagctgt tgttttaact gtaagtgcaa ccgtagcaca aacagaatcg
     3061 gcattgtaga cgcgtaacag tattgctggc actgatatgt tcagtatcgg taatgtttct
     3121 attaactgca cgattaatcc agaagacaac aatttgctac cacactatgt gtggatttac
     3181 ggtcatacct ccacccagct aaatcagtac gattctgata ttatttttat catcaacgat
     3241 aaaaagtatc cggccccatc tgtcgacgga acacgaatga ataagaatgc atgggtatac
     3301 tttatcgatg ctatcagtga agccacgcaa ttcgacgttt tagtaaacgg taagaaagtc
     3361 gacagctata ccgcaaatat caaaaatgtg aaaaagactt tgggtaacaa attctacgac
     3421 agctgctgga acacctggtt ccaggaataa ttgataacgt ggtgtaaaac acctcagttt
     3481 aatttaatac aatgagtgaa aatgaatatt ataaaaatat tttttgtttc agatgtcata
     3541 tattttatta actgaatgat taacaggaga atcagatgaa ctggcattat gaaaaaaatg
     3601 gtgtacgtta tgataacgta acagaggaag atattatcga acgtatccgg cgtggtgaac
     3661 tgaatgcatc aacactggtg tggcaacagg gcatgctgga atggcagcca gtttcagcca
     3721 catcactggc ggaaatactg aaacaaagtg aagttccccc agcccttccg ggtcaccgca
     3781 ttcccggagg cgttgtctgg acgctggcat ttgccccttt gattgggtac atacttgaga
     3841 tgtggactgc agggctgaac ggtatgtcat ttgaacgggc ttatgatgca gtcagcgaag
     3901 gccagtactg gtttatctct ctgattctga atattgctct gggatatctg gatgaacgaa
     3961 aactccgtaa atctggtgtt gatactgcaa catttggctg gctggcatgg ttgatcccgg
     4021 tttatctgtg gcgtcgggca aaaatcctcg gacaaaaacc tgcgtatttc tgggtctggt
     4081 tggttatgct gatactggtg ttatttacgg tctgatattc tttatcgcag agtttactaa
     4141 ataatcaggt tattagtgtt cttaacagg
//
LOCUS       DAESHI010000213         4087 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.216, whole genome shotgun sequence.
ACCESSION   DAESHI010000213 DAESHI010000000
VERSION     DAESHI010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.216"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..877
                     /gene="adeQ"
                     /locus_tag="KW011_004244"
                     /gene_synonym="yicO"
     CDS             <1..877
                     /gene="adeQ"
                     /locus_tag="KW011_004244"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HBH7662415.1"
                     /translation="GLFIALMGLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIIT
                     VLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELA
                     GIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDSVSSVAGAFIGTSS
                     VTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMT
                     SSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCV
                     VVVASLFALKIILVD"
     gene            930..1382
                     /locus_tag="KW011_004245"
     CDS             930..1382
                     /locus_tag="KW011_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HBH7662416.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1593..2773
                     /gene="nepI"
                     /locus_tag="KW011_004246"
                     /pseudo
     CDS             1593..2773
                     /gene="nepI"
                     /locus_tag="KW011_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
     gene            complement(2814..3107)
                     /locus_tag="KW011_004247"
     CDS             complement(2814..3107)
                     /locus_tag="KW011_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662417.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3329..>4087
                     /gene="nlpA"
                     /locus_tag="KW011_004248"
     CDS             3329..>4087
                     /gene="nlpA"
                     /locus_tag="KW011_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HBH7662418.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFLHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQS"
BASE COUNT          899 a          906 c         1077 g         1205 t
ORIGIN      
        1 tggattattt atcgccttaa tgggattaaa aaatactggc gttattgtcg ccaataaaga
       61 cacgctggtg atgattggcg atttaagttc tcacggcgtg ttgttaggta ttttagggtt
      121 ttttattata accgtgttgt catcacgtca ttttcatgcc gcggtgctgg tttctattgt
      181 ggtgacgtct tgctgtggat tatttttcgg tgatgttcat tttagcggcg tctattccat
      241 tccgcctgat attagcggcg tcattggtga agtagatttg agcggcgcgt taacacttga
      301 actcgccggt atcattttct cctttatgct gatcaaccta tttgattcat caggaacatt
      361 aattggtgta actgataaag cgggcttaat agatggtaac ggtaaattcc ccaatatgaa
      421 taaggcgctg tatgttgata gcgtcagttc ggtggcgggt gcgtttatcg gcacctcgtc
      481 tgttactgcc tatattgaaa gtacttctgg tgtggcagtc ggtggccgca cggggctgac
      541 tgcggttgtg gttggcgtta tgttcctgtt ggttatgttc ttctccccgc tggtggcgat
      601 ggttcctcct tacgcaaccg ccggagcgtt aatctttgtt ggcgtgctga tgacttcgag
      661 cctggcgcgc gttaactggg atgattttac cgaatcggtg cctgcgttta ttaccacggt
      721 gatgatgccc tttactttct cgatcaccga agggattgca ctcggcttta tgtcgtactg
      781 catcatgaaa gtgtgcaccg ggcgctggcg cgatctgaac ctgtgtgtgg tggtggtcgc
      841 atctctgttt gcactgaaga ttattctggt ggattagtaa ctggcgaggg tttccagata
      901 tcatgagttc tgataacgca ggagaactca tgatctggat aatgctcgcc acgctggcgg
      961 tagtgtttgt ggttggtttt cgggtgctga catccggggc cagaaaagcg attcgccgtc
     1021 tcagtgatcg gctgaatatc gatgtcgtac ccgtggagtc gatggtcgat caaatgggaa
     1081 agtcagccgg tgacgaattt ttacgttatt tgcatcgccc ggatgagtcg cacctgcaaa
     1141 acgccgcgca ggtgttgctc atctggcaaa ttgtcattgt cgatggtagc gaacagaacc
     1201 tgctgcaatg gcatcggatt ttacaaaaag cccgcctttc cgcgccgatt accgatgctc
     1261 aggtcaggct ggcgctgggt tttctgcgcg aaaccgaacc tgaaatgcag gatattaatg
     1321 cttttcagat gcgctataac gcgttctttc agcctgccga gggcgttcac tggctgcatt
     1381 aaaaccgctt cgtcttatca gcctacagac cgcgcctgac ctgtcgggtg gataagacgt
     1441 tcacgccgca tccggtaatc tttgccctgt tccgtttcga tcttaactcc agctcaagca
     1501 acaccgtaaa agttgcaaaa acgttaaatg cgtcacacat ttcaatgtta aagtggtcgg
     1561 cttttcccct gaaacatgcc acgggtaaca ccatgagtga atttattgcc gaaaaccgcg
     1621 gcgcggatgc catcacccga ccgaactggt cagccgtttt ctcggtggcg ttttgtgtcg
     1681 cctgtctgat tatcgttgag tttttgcccg tcagtttgtt gacgccaatg gcccaggatt
     1741 taggcatttc ggaaggggtt gccgggcaat cggtgaccgt gaccgccttt gtggcaatgt
     1801 ttgccagttt gtttattacc cagacaattc aggctactga ccgccgcaac gttgttattt
     1861 tgtttgccgt tttgctgacg ctctcctgct tgctggtttc ctttgctaac tcattcagtt
     1921 tgcttttaat cggtcgtgcc tgtctggggc tggcgctggg cgggttctgg gcgatgtcgg
     1981 cgtcgctgac catgcgtctg gtgccgccgc gtacggtgcc gaaggcgctg tcggtgatct
     2041 tcggcgcggt ttctattgcg ctggtgattg ccgcgccgtt gggcagtttt ttaggcgagc
     2101 ttatcggttg gcgcaatgtc tttaatgcgg cggcggtgat gggcgtgctg tgtattttct
     2161 ggattatcaa atcattgcct tcactgccag gcgaaccctc gcatcagaaa caaaatactt
     2221 tccgcttatt acaacgtccg ggtgtgatgg cagggatgat cgccatcttc atgtctttcg
     2281 ccgggcagtt tgctttcttc acatatattc gcccggtgta tatgaacctg gcgggattca
     2341 gcgtggatgg cttaacgctg gtgctgttga gttttggcat tgccagcttt atcggtacgt
     2401 cgctttcgtc attcattctt aaacgttcgg taaaactggc cttagcaggc gcgccgttaa
     2461 tactggctgt gagtgcgttg gtgctgacgt tgtggggaag cgataaaatc gttgctaccg
     2521 gcgtggcgat tatctggggg ctaacttttg cattggttcc cgtcggctgg tcaacgtgga
     2581 tcacccgctc actggccgat caggcagaaa aagccgggtc tattcaggtg gcggttattc
     2641 agcttgtggc gcggcaatcg gcggttatgc gctggataat attggtctga cttcgccgtt
     2701 gatgttttct ggcacattga tgttgctgac tgcattgttg gttactgcaa aggtgaaaat
     2761 gaagaaatcc tgatatcagg taaagaagcg ttggtcagta gttgttgctg ttcttacata
     2821 tccgggcatt ctaccttacc caacgcttcc caataattgc tctgattatt acgttccatc
     2881 gccacgatgg cctcgcgtat atgttgttta ttgttttcat ctgacaacat taacttttcc
     2941 cacgcttcat ctgataatga cgcctgtggc gtgcaaattt tacgtaaatc ttgtaatacc
     3001 gtggttttct cttttgcgga ctgcagtgag ctaaaaggcg actcggcgta tacgatgcct
     3061 ggcatagcga aaaaagtgaa aataagcagt agtgtcgttg gtttcatgga ttcaaccttt
     3121 tacaaatgtg tatcgaatga agcataagtg tagtttgctt tcatcgctct catcatgctg
     3181 tacgaaacgc gcttgcgtgg tcagtaagaa gtgccagact ttatattcca cttttattcc
     3241 tttttattct tatcgatagc gtttcgtttt ttaaaccgca gcgaccttac cgctatagtc
     3301 aggtaatcat taataaaagg ataaaaaaat gaaactgaca acacatcatc tacggacagg
     3361 ggccgcatta ttgctggccg gaattctgct ggcaggttgc gaccagagta gcagcgatgc
     3421 aaaacacatt aaagttggcg ttataaatgg cgcagaacaa gatgtcgcgg aagtcgctaa
     3481 aaaggtggca aaagagaaat atggcctcga tgttgaactg gtaggattta gcggttcgtt
     3541 gctaccgaac gatgcgacaa atcatggcga acttgatgcg aatgtcttcc tgcatcgacc
     3601 ctttcttgaa caagataatc aggcgcatgg ttataaactg gtagcggtgg gaaatacgtt
     3661 tgtcttccct atggcgggtt attccaaaaa aatcaaaacc gtggcgcaaa taaaagaagg
     3721 cgcgacagtg gcgatcccca acgacccgac caaccttggt cgcgcacttt tactgttgca
     3781 aaaagagaag ctaattactc tgaaagaggg aaaagggtta ttacctaccg cgctggatat
     3841 aaccgataat cctcgtcatt tgcagattat ggaactcgag ggggcgcagc tcccgcgcgt
     3901 actggatgat cccaaagttg atgtagcgat tatcagcacc acttacattc agcagacagg
     3961 gctttctccg gtgcacgaca gcgtatttat tgaagataag aattcgccgt atgtgaatat
     4021 tttggtggca cgggaagata ataagaatgc agaaaacgtg aaggaatttc tgcaatctta
     4081 tcaatca
//
LOCUS       DAESHI010000214         4079 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.217, whole genome shotgun sequence.
ACCESSION   DAESHI010000214 DAESHI010000000
VERSION     DAESHI010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.217"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            171..506
                     /locus_tag="KW011_004249"
     CDS             171..506
                     /locus_tag="KW011_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH7662419.1"
                     /translation="MLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTED
                     TIDGLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLT
                     LSYREVNRD"
     gene            499..981
                     /locus_tag="KW011_004250"
     CDS             499..981
                     /locus_tag="KW011_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662420.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(1015..1095)
                     /locus_tag="KW011_004251"
     CDS             complement(1015..1095)
                     /locus_tag="KW011_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662421.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(1131..1555)
                     /locus_tag="KW011_004252"
                     /pseudo
     CDS             complement(1131..1555)
                     /locus_tag="KW011_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1985..2475
                     /locus_tag="KW011_004253"
                     /pseudo
     CDS             1985..2475
                     /locus_tag="KW011_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2700..3191
                     /locus_tag="KW011_004254"
     CDS             2700..3191
                     /locus_tag="KW011_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBH7662422.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
     gene            3526..3984
                     /locus_tag="KW011_004255"
     CDS             3526..3984
                     /locus_tag="KW011_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBH7662423.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDG"
BASE COUNT         1522 a          654 c          661 g         1242 t
ORIGIN      
        1 agatccataa tgacattaac atacaaaatc atgtaataaa ctcgtcaaca gacgcacata
       61 cacacaatgc cccacctgcc ataaaagtta atgcgggata caaagagagc attggtggcg
      121 caaagaattt caataacaaa atcctcaaac atataaaaag tcatctaatt atgttgagcg
      181 catttgtcat aggcgcatat tctgcatatt ggctatggaa taataatcaa ccgaagccat
      241 ttttcaagga ttacaagact gttgcggaaa ttaatggctg ccattttaat gtaacagaag
      301 atacaattga tgggttgaaa gagttcgata aatataaaac acggatactg gattcgggga
      361 ttaactgtaa aaaatatccg tggttatact tccctcttgc aaaatcctca ccggggatga
      421 ttgttatggc atgcaataaa aactataacc aacatgaagt ggcagactgt ttaaccctgt
      481 cttaccgtga ggttaatcgt gattgattat aaaaaaaact tattatttat tttagttttt
      541 atcagtgggt ttattctctt cactgtctat tcttacactg cagaaaaaat gatatataac
      601 gaaacatgca ctgcaaattg ggtaattttt aatgaccagg gacgagcaaa tcttaccatc
      661 gactttatgt ataacaaaaa aaataaaaca ggaactgttg cgttgagtgg aacctggcaa
      721 caaggtaaca gagaaagtaa atcaatacgg cgaaatattg aatacacatg gattgaaaac
      781 tatgacacag cccatttaac atcaaagaaa gttaataagt ttgagattat ggatcaagtt
      841 gatgatgata gacttgcaca acttattcct gatttttatg tctttccaga aaaaagtgta
      901 agctataata tactaaagca aggtaagcat gcttttattt tgagcattgg taacagagca
      961 ataatgcatt gtgcaaggta aactaaaata gataacaaaa aagatggaac ctcgttaatc
     1021 ataaaacaat aaaattaaat attctcgcag tatatggcag tctaaagcat caaagttttg
     1081 atcaacatct ttcattttag acatctcctt gcaatgtaaa atatcatgta tcaacaacat
     1141 ctggtgcaaa tccatgagtc gaactcgaca ttcatcttag ctcgattatt attccttcgt
     1201 acagtcgatg taaacaatac agaaagagga ttattaagaa ccagtttact caggaatcaa
     1261 tagaggaaag gactaacgtt tctttaaaag aattgatttc atcatctgtt aaactaaact
     1321 catcattgac agatcgtgag atataactgt ttttaacttt actctttacg ttgactttat
     1381 tgacagaatt aacattcaca tatcttgaat ttaatgtcca tgatgttgtt tgcgtgagaa
     1441 cattctcagc attaaggaat ttttttaacg gaatccttgg tttctttttc gagtccgaat
     1501 ttacaatatc atgcatatag accatttcat gaatctgcga aaactcaatg tccattgtat
     1561 acctcacatt tttaccgtga ctcgatgtta ctgttcaata atcaccttcc atcaatacta
     1621 aaattaatac ccctaatgtg ccgataacaa atatagtcat tctacgtaac gtctccataa
     1681 ggtgataacc ttgatcaaat gaatttcgaa tataataaag gaaatctttt gagatgttta
     1741 tcacgaataa agctatttat gttaccaata tcatttcgag tgcgcacact atctatctgt
     1801 tttttcgaga tataaaaaac aacgtgtaag ttatgtaaat tgccgatgct actgagtaac
     1861 attatggtaa aaaaagtaaa aatgagcgat ttttgataga ataaagcatg agaaaaggtg
     1921 atatttgaca ttatcagaag ctgcgaattc ggattttgct ctaatcaaat gagagaaatg
     1981 taaaatgaag ccacgaaata ttaataatag cctaccactg caaccattag ttcctgatca
     2041 ggagaacaaa aataagaaaa atgaagagaa atccgttaat ccagttaaaa tcacaatggg
     2101 gtctggttta aattatattg aacaagaatc tcttggagga aaatatctaa cacatgattt
     2161 gtcaataaag atagcggata tttctgaaga gataattcag caagcaatat tatctgctat
     2221 gagcatatat aaattttcga taacagatga tttaatgagt atggctgtaa atgaactcat
     2281 aaaactgacc aaaatagaga ataatgtaga cctgaataaa ttcactacta tatgcacaga
     2341 cgttctatcc cccctcgtca ccagacataa taaagaaaaa aaacaaacga cattctaccc
     2401 ttctcaaaaa tccccttttt aattttcatt gaaaaaatgg caattgatac tatgaatcca
     2461 ggtcatacac attaaggcat ttatgtaatg tcgtaattaa gataactaat aaggtgaata
     2521 ttagtaaaac aactaatgat gttattagtt tgtagtgaac agtactttta ccaataatga
     2581 aaaatatacc ataagcaatg caagcttaaa actaataatt taaaaaagat aaatgtaaaa
     2641 atattaatga gtcaatcaat aataatttag catcacgaga cacatcacag aggaatatta
     2701 tgagcacaga aacaattgaa atattcaata atagtgatga atgggcaaat caactaaaac
     2761 acgcattatc gaaaggagaa aatctggcat tactacatgg tttaactcct gatatccttg
     2821 atagaatata tgcatatgca ttcgactacc atgaaaaggg taatataacg gacgcagaaa
     2881 tttattataa atttttgtgc atttatgcgt tcgaaaatca tgagtatcta aaagattttg
     2941 catcagtatg tcagccaaaa aagaaatatc aacaagcata tgacctttac aaactaagtt
     3001 acaattactc cccgtatgat gactattcag ttatttatcg tatgggtcaa tgtcagattg
     3061 gggctaaaaa tatcgataac gcaatgcaat gtttctatca cattattaac aattgtgagg
     3121 atgatagtgt taagagtaaa gcgcaggcat atattgaact cttaaacgat aattcagaag
     3181 ataatggcta agcattacaa tcatttggtt aaatttgctg gttatagtgc agttaactac
     3241 tataaccagc acctcttatg ttttcttaaa tcagcatctt aaaacagtta agagcatact
     3301 ataaatcatt atttttcaat gcgttagaga acaatctact ttataaaaat tgaataaggc
     3361 tatttcagat tacatcactg aattcctaag ataaattagt gaacacaacc atagatatgt
     3421 cgaatgtatt ttcctgtagc attaatgaca cataaatatg aatagccata atttctattg
     3481 cttaagcagg atgcaagaaa ccaatttttt catagaggtt aactaatgga cttagaaaat
     3541 aaattctcat atcattttct tgagggatta acgctcacgg aagatggaat tcttactcaa
     3601 ggaaatgagc aagtttatat tccacagaaa gagttaggtg tattaatagt attacttgaa
     3661 tccgctggtc atgttgtact gaaagatatg atcatcgaat cagtatggaa aaatattatt
     3721 gttagtgacg agtccctgac aagatgtatc tattctttgc gctgcatttt tgaaaaaatt
     3781 ggctatgatc gttgcataga aacaatctac cggaaaggtt atcgtttcag cgggcaggtt
     3841 ttcaaaacta aaattaatga agataatact tcagactatt ccatagctat attccctttc
     3901 actacttcat tgaatacact ggatccatta atacttaatc aggaattagt gcaaatcatt
     3961 tcaaataaaa aaatcgatgg ttagactggc cccctgaatc tccagacaac cagtatcact
     4021 taaataagtg atagtcttaa tactagtttt tagactagtc attggagagc agatgattg
//
LOCUS       DAESHI010000215         4075 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.218, whole genome shotgun sequence.
ACCESSION   DAESHI010000215 DAESHI010000000
VERSION     DAESHI010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.218"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..784
                     /locus_tag="KW011_004256"
     CDS             <1..784
                     /locus_tag="KW011_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBH7662424.1"
                     /translation="AKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVN
                     QQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQLS
                     DTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQG
                     NRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGS
                     RLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            797..1756
                     /locus_tag="KW011_004257"
     CDS             797..1756
                     /locus_tag="KW011_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HBH7662425.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGRSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNYNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(1794..2693)
                     /gene="nhaR"
                     /locus_tag="KW011_004258"
     CDS             complement(1794..2693)
                     /gene="nhaR"
                     /locus_tag="KW011_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HBH7662426.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2759..3925)
                     /gene="nhaA"
                     /locus_tag="KW011_004259"
     CDS             complement(2759..3925)
                     /gene="nhaA"
                     /locus_tag="KW011_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HBH7662427.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1224 a          952 c          861 g         1038 t
ORIGIN      
        1 tgccaaaaca cgtcaccaaa ttaacttatc gaattcgacc tcattttcca aagatggcta
       61 ttcctccaac aatactggaa ttactggcat agccggtgaa catgatcagt taaattatgg
      121 tatatatgtt aatcagcaac aacaaaataa tgatacctcg cttggtacga atttaagctg
      181 gagaactccc atcgccataa tagatggcag ctatagtcat tctaaaaacg cctggcaaag
      241 tggtggaagt attagtagtg gattagttgt ctggcccggt ggtattaata tcactaacca
      301 gttatccgat acatttgcaa ttctggatgc gcctgggtta gaaggtgcac atattaatgg
      361 acaaaaatac aatcgaacaa acagcaaagg ccaggttgtt tacgacctga taatacctca
      421 tcgtgaaaac catctggtac ttgatatagc aaacagtgaa agtgaaacag aattgcaggg
      481 caatcgtcaa attattgcac cttaccgtgg agcagtttct tatgtgcagt ttacaactga
      541 ccaacgtaag ccctggtata tacaggcact gcgtcccgat ggttcgccat taacctttgg
      601 ctatgacgta ctggatctcc aggaaaataa tattggagtc gttggccagg gtagtcgcct
      661 ttttattcgc gtagatgaaa ttcccactgg aataaaagtt gctctcaatg atgaacagaa
      721 cttattctgt actattactt ttcaacacgt tatcgatgaa aacaaaacat atatatgcca
      781 gtaaaaggag tttactatga agtggctttt attaatcacc ttgagtctat attcttttat
      841 tgtgcaaagt gctccctgtg cattgaccaa tgtcggggaa caacgtggaa catatatact
      901 caagccactg agtatgaaag gtaatctcac cgccgaaaaa attgaaaaca ccggaaatat
      961 cattgagtgt gcagacttac aagagacgac caacgccatt aaagttcatt ttttaacaca
     1021 gaacgcttta aggtttaaag tcggaagtcg tcattatatt attaatttcc cctttgaatc
     1081 tgacggtaaa aaaatagagc ttccaggaag gtcatcttat aatttagaag aaatgctatc
     1141 tgcgataaat ttcaccattc catactctat cgcatctgta tcagatagtt ccaacaccgc
     1201 agatattttt ccaggaactc cttttccttt gttgttcgaa attgaagtga gttcatgtac
     1261 agggaataat actaattgta atagcgtccg ctttaattat aatttaaata ctattttaca
     1321 ggtaaattta atgacctgtg gttttgaaga tcaatccatc gacactggac gatttgtact
     1381 gtctcgtatc aataatgaaa attatcagtt tcatcatata agatttgact gcatccaggg
     1441 cgagcaaggt gaattagttt ttgaacccag tgatgttgaa tattactttg aaccagtgac
     1501 gatacaggag tcaggaacca gcatattaaa aaatgagtta gaaaacagtg aggacggtgc
     1561 gggtcaagta ggctttcaac tctccaatga tggtacgaat gaaatccagt atggtaaaag
     1621 caattactac tcttttcagc atccccatga aggaagtaat cagattcctc tatttattcg
     1681 cccgcgtaca tatggaaata atgtctcaag cgggcaaata atgagccgag tcaaaatcgt
     1741 ggttatgtac aactaatcta tctcccccac cttaactttg ggggaggtgc cgattaacgc
     1801 gccgctggac taaaaagcgc agaataatcc gtattgcaga ttcgctgtac cgccgggtgc
     1861 tgaatcatcc gctcagcaaa aatagcatgg tactcttcca tcacattctc gacgcgacca
     1921 atttctacga cagttttatc ggcataaaag tcatatgcat aaagcgttgg ggcaacgaag
     1981 attgcattgt gcatcgcacc aaaagctttc atcaacgcgg catcatcaaa ctcgccgagg
     2041 atttctacgt ttaatccctg ggagttaaac cagttaagca atttgcgccc taacattgaa
     2101 cgtcgcccag gaatcaaaag tcgccgttct tccagacaag ccgggaacga tttttctggt
     2161 ggtggatttg tacaccagaa actcacgcca cattcgccaa ttctcacgga gaacaggcct
     2221 tcctgctgcg tagagtctat cggacagtca gaaatgatca tatccagttt atgctgactt
     2281 aattgctcca gcagcatttc gtgggtggat tcgaagcagc gaagatgaat gggctcgcct
     2341 tctaccactg cggcgttaag tacgctactg accaggcgtt tggaaagtgc atcagccacg
     2401 ccaacgtcaa acaataaatt ggattctttg cgatagttca caatatccag catttcctgg
     2461 cttaaggtga acattttatc ggcatagcga tagaccagtt ctcccagctc gctgggttcg
     2521 agaccacgtc ccttgcgttt aaataatttg ccttgcaggc gctcttccag cgctcgaatc
     2581 tgtccggtaa tggtttgtgg agttaaataa agcgcctccg ctgcgccaac cacggaacct
     2641 tctttataga catgccagaa gtaatacaag tggttgtaat tgatatgaga catgctcatt
     2701 tctctccctg ataacaatga aaagggagcc gtttatggct ccccggtaaa ccgtcctgtc
     2761 aaactgatgg acgcaaacga acgcgtaacc agctgtatcc aattaccgcc gaagagatag
     2821 aaccgaccag gataccgagt ttcgcccagt taatcagttc tggatctacg ctaccaaagg
     2881 ccaggctggc aataaagata gacatagtaa aaccgatacc gcacaggatc cccaccgcca
     2941 taatttgctg ataagtcgtt ccctcaggca gatgcgccag tttcaaacgc agcgccaacc
     3001 agcagaacag actaatcccc agcggtttgc caatcagcaa gccagcgatg atccccaatg
     3061 gcagaatgga ggtcaagcca tccagcgtga cgccttgcag tgaaacgcca gcattagcaa
     3121 atgcaaacag cggtaaaatc agatacgcca cccacgggtg caatacatgc tccagtcgct
     3181 tcgctggaga acgcccatgc ttctctttca aaggaataaa gaagccgaca attacccccg
     3241 ccagggttgc gtgaaccccc gatttcaaca ccgcagtcca caacaccacg ccaacaagaa
     3301 tatagacgcc cgtgcggcgt acaccacaca gattcaatac cgcgagtacc gcaattgcta
     3361 cagccgcgac gccaagagag gccatcgata agtcattagt gtagaacaat gcgatgataa
     3421 tgatggcccc aagatcgtcg ataatagcca gagccatcaa aaagatcttc agtgctaacg
     3481 gaacacgact tcccaacagc gccagcacac caagtgcaaa ggcaatgtca gtagccgccg
     3541 ggatcgccca cccttcgcgg gtaatcggat cggcatagtt aaaggccaga tagagtaatg
     3601 ccggaacaat catcccacca atagcggcga taaccggaaa tgcggcctgg cgtaagctgg
     3661 ctagcgatcc ttgcatcagt tcacgtttaa cttccagacc gactaacagg aaaaataccg
     3721 ccatcagcgc gtcatttatc cataacagca tgtttttgtt gatttcgagt gaaccaaccc
     3781 ggagctgaac cggcgtctcc agaaagtcgt gataccatcc actggttgcg ccgctgttgg
     3841 ccatcatcat cgccaggata gcggcaatga taagaataat gcctcccgag gcatcactgc
     3901 taaagaatcg atgcagatgt ttcacttttt atttctcttt caggtgaata gatcgttttt
     3961 actattttac cccgcacaaa tcatcgttaa aatcagatta tagtgtagga ataactcgtt
     4021 ttttacgagc aattaaagga tttgcgccat cagcggtgag atgagttttt gacgg
//
LOCUS       DAESHI010000216         4049 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.219, whole genome shotgun sequence.
ACCESSION   DAESHI010000216 DAESHI010000000
VERSION     DAESHI010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.219"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            31..759
                     /gene="mpaA"
                     /locus_tag="KW011_004260"
     CDS             31..759
                     /gene="mpaA"
                     /locus_tag="KW011_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HBH7662428.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(734..1699)
                     /gene="ycjG"
                     /locus_tag="KW011_004261"
     CDS             complement(734..1699)
                     /gene="ycjG"
                     /locus_tag="KW011_004261"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HBH7662429.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1818..2324
                     /gene="tpx"
                     /locus_tag="KW011_004262"
     CDS             1818..2324
                     /gene="tpx"
                     /locus_tag="KW011_004262"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HBH7662430.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2368..3909)
                     /gene="tyrR"
                     /locus_tag="KW011_004263"
     CDS             complement(2368..3909)
                     /gene="tyrR"
                     /locus_tag="KW011_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HBH7662431.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICVTQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVQRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
BASE COUNT          970 a         1143 c          972 g          964 t
ORIGIN      
        1 cctgtaatac ttcctgaacc agagacgatc atgaccgtaa cccgcccacg cgccgaacgc
       61 ggcgcatttc cgcccggaac agaacattac ggacgttcat tattgggcgc gccgttaatc
      121 tggtttcctg cccctgcagc cagccgcgaa agtggtttga ttctggctgg cactcacggt
      181 gatgaaaact cttcggtcgt cacgctctcc tgtgctctgc ggacgttgac accttctttg
      241 cgccgccatc atgtggtgct gtgtgtgaat cctgacggct gccagctggg gttacgggcc
      301 aatgctaatg gtgtggattt aaacagaaac tttccggcgg cgaactggaa ggaaggtgaa
      361 acggtttatc gctggaacag cgccgctgaa gaacgtgatg tcgtattact gacaggtgac
      421 aaacctggct cagaacctga aacccaggcg ctgtgccagc ttatacaccg cattcaacct
      481 gcctgggtgg tctcattcca cgatccactg gcctgtattg aagatcccag acacagcgaa
      541 ttaggcgaat ggctggccca ggcgtttgaa ttgcctctgg taaccagcgt cggttatgaa
      601 acgcctggct ctttcggcag ttggtgtgca gatctcaact tgcattgcat caccgcagag
      661 tttccgccca tctcctccga cgaagccagc gaaaaatatc tctttgcgat ggcaaacttg
      721 ctgcgctggc atcctaaaga tgcaattcgc ccgtcgtgaa ctgaagcgcc ggttccacat
      781 ctaccgccag ccaggtcggt ccgtcaagat cggcgaaact gacctgcggc accagcggta
      841 aagcggcgct aatggcacga gaggtacaca acatgcagcc cagcatcaga ctgaaacctt
      901 gtgcacgcgc ttcagtcgcc agcgccagcg cttccgtcag accaccgatt ttatcgagct
      961 taatgttaac catctcatag cgccctttca gcgccttcag attgctacgt gtatgacaac
     1021 tctcatcagc acaaatcggc aacggatgaa taaaattctc cagcgccgca tcgtcctgcg
     1081 caggaagggg ttgttcaagc atcgcaacgc ctaaatccgc caatagctgg caacgcgccg
     1141 ccaacccttc tgcacgccag gattcatttg catcaacgat cagcgtcgca tcgggcacag
     1201 ctgtgcgaat tgccaccatc cgctcactaa taagatggtt atccagcttc actttcagta
     1261 atttcgcgcc tgcctgccag agtgttgatg cactattggc catctgatca ggcgtaccga
     1321 tgactaccgt ctgtgcagtg ataactgtct cgggaagcgt tatgccgatc aaatcagcca
     1381 gcgattgctg ctgttttcgc gcggccagat cccacaatgc acaatccagc gcattacgtg
     1441 ctgcgccggc agggagaatt ttttgcaact cctcccgtgt cagccctttc tctaattgcg
     1501 acacgacgct cataatttgc gccattaccg aggcatcact ttctccataa cgcggatacg
     1561 gcgtgcattc gccggtgcct ttaataccct cttcttccag ttcaaccacc accacgcgcg
     1621 cttcactgcg acttccccgg gcaatcacaa acggggtatg taagggccag gcttcctcaa
     1681 ataccttaac ggttctcatc tttactcctt gttgtcgcgg tggtctgcgg attaagcgca
     1741 aatagcgttt gctgtgttat tgacagttag cataaactag gtgtgacgtt aactatatgt
     1801 aaacaggaaa gataatcatg tcacaaaccg ttcatttcca gggcaacccg gttacagtcg
     1861 ccaattccat cccgcaggcg ggtagcaaag cgcagacttt tactctcgtg gcaaaagatc
     1921 tgtctgacgt caccctcggt cagtttgcgg gtaaacgcaa agtgctgaac attttcccga
     1981 gtattgatac cgatgtttgc gccgcatcag tacgcaagtt taaccaactg gcaactgaga
     2041 tcgacaacac cgttgtgctg tgtatctctg ccgatctgcc gttcgcccag tctcgtttct
     2101 gcggcgcaga aggtctgaac aacgttatca ccctctccac tttccgtaac gctgaatttc
     2161 tgcaagctta cggtgtggca attgctgatg gcccactgaa aggtctggca gcgcgtgccg
     2221 ttgtggttat tgacgaaaat gacaatgtga ttttcagcca gctggtggat gaaatcacca
     2281 ccgagccgga ttacgaagca gctctggctg tactgaaagc ataagttaat atgcctgatg
     2341 gtgttgcacc atcaggcata ttcgcgctta ctcttcgttc ttcttctgac tcagaccata
     2401 ttcccgcaac ttattggcaa tcgcggtatg tgaaacgccg agacgttttg ccagtttgcg
     2461 cgtgctggga taattgcgat aaagctgggt taataccgag cgttcaaaac ggctggtgat
     2521 ttcgtccagc gaaccttcca tcgcatcttc gcccacggct accgttgcgg cgtcataatc
     2581 cggcaacaaa atatcctgtg gacgcagctc ataaccgtcc agttgtgtca gtgcgcgata
     2641 gatagcgttc tttaactgcc gcacatttcc cggccacgca taacgcgtaa gtacagtatt
     2701 caggtcagcg gccagtttcg gacgctgcac gccctgctcg tcggcaaagc gggcgacgaa
     2761 cagctcagtt aacggcatga tgtcctgcgg acagtcacgt agcggcggca gattgagcgt
     2821 caacacgttc agacgataat agagatcttc acggaacatg cctttttgca ccagttcgac
     2881 cagattcttc tgcgtaacgc aaatcacccg cacatcgaca tgcacctcat ggtcttcgcc
     2941 aacccgacgg aaagtgccat cattaaggaa acgcagtaat ttcgcctgca tccgtggtga
     3001 catttcccct atttcatcca acagcaccga accaccgttc gcctgctcaa agaatccttt
     3061 cttcccttcc ggagcatgac caaacagttc actctcgacc gcatcttccg gtatagacgc
     3121 acagttcagc gccaggtaag gtttgcccgc tctggggctt gcctgatggc aggcgtaggc
     3181 aaagagatct ttacctgtac ctgtgtcacc cgtaatcagc agcggcgcgc ttagcatcgc
     3241 cagtttctgc gcctgttcga caacatgctt cattttcggg ctgacggcga caatttgact
     3301 gaaggcgctg acgtcctggg cggcgacatt ttgcaactgg cggcccatac gaatcgttga
     3361 tcgcaacatc accaccgcac cggtcaggac gtgttgatca ttttcatcct gaagataaac
     3421 aggcgtaatc tccatcagga aattctgccc attaataacg acatgctcgt tatgcgaatc
     3481 ttgcggttcg ctttccagcc aacgtaaaaa attaaagccg ttaatcaatt gtgcggcggt
     3541 atggttgcgc aggcgatcca atttttgccc aaaaagctga cagctcgccg ggttcgccat
     3601 atccactttg cttttcatat cgacagagag cacaggttca ggcaacgcct ccagtaacgc
     3661 gctcaacgcc agatgctcac gttcggaagg catccacggg acagtacgca catcggtaac
     3721 acccgcaata cggcgtattt cggccatcag actgctgaaa ctctcaaact ccagttcagc
     3781 aaaattgagg tagattcgcc caatgggatc aatctcaata ccgcgtaaat caatgcctct
     3841 tagcacgagt agatcgagta attcgcgggt cagaccgagt cggtcttcac aaaagacttc
     3901 cagacgcatg ggaaccttca cctgaaaaaa gaacaattaa tatgatgata tgacactatt
     3961 tggatagcag gaaggtttct gtcaacaatc attgacacaa agacggaaaa acgggcactt
     4021 cggcgtaaag atatccggtc agcacccga
//
LOCUS       DAESHI010000217         4041 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.220, whole genome shotgun sequence.
ACCESSION   DAESHI010000217 DAESHI010000000
VERSION     DAESHI010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.220"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            924..1640
                     /gene="nanC"
                     /locus_tag="KW011_004264"
     CDS             924..1640
                     /gene="nanC"
                     /locus_tag="KW011_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HBH7662432.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1660..2766
                     /gene="nanM"
                     /locus_tag="KW011_004265"
     CDS             1660..2766
                     /gene="nanM"
                     /locus_tag="KW011_004265"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HBH7662433.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2831..3811
                     /locus_tag="KW011_004266"
     CDS             2831..3811
                     /locus_tag="KW011_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HBH7662434.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         1219 a          845 c          913 g         1064 t
ORIGIN      
        1 agataagggg gaacgcagga gacgcttttt catctacatt cgtgggtaaa tatggctttg
       61 gtcactgcga cttaaacctt cctcaatcac agctattcgc cgggcgatac ggcaaacaaa
      121 ctaagtgaaa catcatcctg aaaacaccaa catcaacaag cctctctaga tcgacttcag
      181 aagcgaccag ttacaagcca caaatcagga aacatatttc atggtgagaa ttatgatcag
      241 cctgatggcg ggtaaacact tgttttcacc acgctcactg agttagcttg caaagcttcc
      301 cttgtttaga ccctaaataa attttttata aaacaaacag ttaaaagaaa tcctttagtt
      361 tttgaagacc acctcaagtg ttcgtctggc ttcacatggc atcttcctct tagaaaaaga
      421 tcgacatatt ttgtgacacg aattgcaaat ctcgttttgt tgtatggctt gcgtgatttt
      481 tgatctggta taacaggtat aaaggtgcac cgcgatagtc aatgagacag ggcatctcgc
      541 aatctatggc aaacatcact tcagttcttt ctcatcgggt gatgaaaacg cacttcagtc
      601 tgaaaggaat atgaaaatga gatcaacaga cactctattt tatgactctg ggtaaaatgg
      661 attgagtaag tgatatagct tacgaacatt caaatcaatt aaacatcaga agagatttta
      721 tactcaggta tttaatctga ttctctgttt atttaaataa tgtggaaaga gaattttcac
      781 aggagacctt atacaaaaat ataaaataag ctacccgttg ccaaagacac tataagcctg
      841 gcaaaaaata ttacacaaca taaatgctaa ttgtttatgc gggctttgta ttgctttctg
      901 tatcctacaa atgagtgaaa tttatgaaaa aggctaaaat actttctggc gtattattac
      961 tgtgcttttc gtcaccatta atttctcagg ctgcgacact ggacgtacgt ggtggatatc
     1021 gtagcggaag ccatgcctat gagactcgac tcaaagtcag tgagggatgg caaaatggat
     1081 ggtgggcaag catggaaagt aatacctgga ataccattca tgataataaa aaggaaaatg
     1141 ccgcactcaa tgatgttcag gttgaagtta attacgcgat taaacttgat gatcaatgga
     1201 cggtgcgccc gggaatgtta acgcatttta gcagcaacgg cacacgctac ggaccctacg
     1261 taaaactgtc ctgggacgcg acaaaagatc ttaattttgg cattcgctat cgttacgact
     1321 ggaaagctta ccgacaacaa gacttatccg gtgatatgtc tcgtgataac gttcatcgtt
     1381 gggatggata tgtcacttac catattaata gtgatttcac cttcgcatgg caaacgacgc
     1441 tatacagcaa acagaatgat tatcgctatg caaaccacaa gaaatgggcg acggaaaatg
     1501 cctttgttct acaataccat atgacgcccg atattacgcc atacatagaa tatgactacc
     1561 ttgaccgcca gggtgtttac aacggcagag ataatttatc ggaaaacagt tatcgcattg
     1621 gtgtgtcatt taaactgtag tagacaggag acagtcacaa tgaataaaac tataatggcg
     1681 cttgctatca tgatggcttc atttgccgca aacgcgtctg tattaccgga aactcctgtg
     1741 ccatttaaaa gtggtaccgg agcaattgat aacgacactg tctacattgg tttaggtagc
     1801 gcaggtacag catggtacaa gctagataca caggccaaag ataaaaaatg gactgcgtta
     1861 gctgcattcc ctggtggacc gagagatcaa gcaacctctg catttattga tggcaatcta
     1921 tatgtgtttg gcggcattgg caaaaacagc gagggattga ctcaggtatt taatgacgta
     1981 cacaaataca accccaaaac caatagctgg gttaaattga tgtcgctcgc gccgatgggc
     2041 atggcgggtc atgtgacttt tgtacacaac ggcaaggctt atgttactgg cggtgttaac
     2101 cagaatatct tcaatggcta ttttgaagat ctcaacgagg ctggaaaaga ttcaaccgct
     2161 atagataaaa tcaacgccca ctattttgac aaaaaagcag aagattattt cttcaataag
     2221 tttctgttgt cttttgatcc ctcaacacag caatggagtt acgctggcga atcgccctgg
     2281 tacggaacgg ctggtgcggc ggttgtgaat aaaggtgata aaacctggct tattaatggc
     2341 gaagccaaac caggattgcg aacggatgcc gtatttgaac ttgatttcac cggtaataat
     2401 ttaaaatgga ataagcttga tcccgtctca tcaccagatg gcgtcgctgg cggttttgcg
     2461 gggataagca atgattctct tatatttgcc ggaggggccg gattcaaagg ttcacgagaa
     2521 aattaccaga acggtaagaa ctatgcgcat gaaggcctga aaaaatcata tagcactgat
     2581 attcatcttt ggcataacgg gaaatgggat aaatcgggtg aattatcgca aggtcgggcc
     2641 tacggagtat cattgccctg gaataatagt ttattaatta ttggcggtga aactgcaggc
     2701 ggcaaagcgg tgacggattc agttttaatt tctgtgaagg ataataaagt cacagtacaa
     2761 aactaacgct tcagggcccc ggtaaagggg ccctgctatc aatttgtcat ttgtattacg
     2821 cgaaaattat atgaacgcaa taatatcgcc cgattattac tatgttctta ccgttgctgg
     2881 tcagtctaat gccatggcgt atggcgaagg actgccatta ccggacaggg aagatgcgcc
     2941 tcattccaga attaaacaat tagcgagatt tgcgcatacg catcccggag gcccgccatg
     3001 tcactttaac gacattattc cactgactca ctgcccacac gatgttcagg atatgcaggg
     3061 ttatcaccat cctctggcaa cgaatcatca aacacagtac ggcaccgttg gccaggcact
     3121 gcatattgca cggaaattac tgcccttcat tcctaataat gcagggattc ttatcgttcc
     3181 gtgttgccgt ggcggatcgg cttttaccgc gggcagcgaa gggacatatt cagaacggca
     3241 cggagccagc catgatgctt gtcgttgggg aacggatact ccgctgtacc aggatttagt
     3301 cagcagaacg cgagccgcac tggcaaaaaa tccgcagaac aaattcctcg gcgtatgctg
     3361 gatgcaaggc gaatttgact taatgaccag tgactacgcg tcacaccctc aacactttaa
     3421 tcatatggtt gaagcctttc gtagggatct aaaacaatac cattctcagc ttaataatat
     3481 tactgacgcg ccatggtttt gcggcgatac cacctggtac tggaaagaaa atttcccgca
     3541 ttcgtatgaa gctatttatg gcaattatca aaataatgtt ttagccaata ttattttcgt
     3601 cgacttccag caacaaggtg aaagaggact gacgaacgcg cctgatgaag acccggacga
     3661 tttaagcacg ggatattacg gttcagcgta ccggtcaccg gagaactgga cgacggcact
     3721 gcgaagcagt catttcagca cggcagcccg tcgggggatt atttctgaca gatttgtaga
     3781 agcaattttg cagttttggc gcgaaaggtg agcggggagt ttattcatgg gggatgtcct
     3841 tgttcgttct cagagtagac aacaaaaaac aaataagatc aaggtaatat ctcttcactt
     3901 gcgggctcga acagcaggca attgccaata taataatctg gcacaagggt gaaaaacgcg
     3961 caccgcataa accgttgtta ttgcttacag attagccgga tgccttaata gacatccgcg
     4021 ccttttcgat tatggctcgg a
//
LOCUS       DAESHI010000218         4022 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.221, whole genome shotgun sequence.
ACCESSION   DAESHI010000218 DAESHI010000000
VERSION     DAESHI010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.221"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..366)
                     /locus_tag="KW011_004267"
     CDS             complement(<1..366)
                     /locus_tag="KW011_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBH7662435.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCMELVASGDVYNSGYGVFPSEQAHKDWQNARKKLSRVKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSE"
     gene            complement(381..1127)
                     /locus_tag="KW011_004268"
     CDS             complement(381..1127)
                     /locus_tag="KW011_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH7662436.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLADAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            complement(1134..2333)
                     /locus_tag="KW011_004269"
     CDS             complement(1134..2333)
                     /locus_tag="KW011_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HBH7662437.1"
                     /translation="MLFVLILSNRAASYGAIMAALPYMQLYIADYLADTMHLSAEEHG
                     AYLLLMFNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEED
                     LASVREKLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVMFEHDANTKAT
                     NKDTDKDLKTDLTHPKPFPSGREFRDFVAGVLEGRLSGGTAAEFCNSAVVALQAAGLD
                     VCREYPVPERGDGCGGRIDIVVTDRNGVRCGSELDRNSPRQKSLLKIGAVETGICVLR
                     RSDIARHTEQGILVIGGAVRQKKFDPLSVDLPDWLPETLWHEWVQFRQALRKPIRTEQ
                     GANGAIRELEKFRQQGFTPEQVIRHSIANEYQGLFAPKGVRPETLLRQVNTVSLPDSA
                     IPPGFRG"
     gene            complement(2346..2768)
                     /locus_tag="KW011_004270"
     CDS             complement(2346..2768)
                     /locus_tag="KW011_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662438.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSDDSVIVH"
     gene            complement(2765..3007)
                     /locus_tag="KW011_004271"
     CDS             complement(2765..3007)
                     /locus_tag="KW011_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH7662439.1"
                     /translation="MNKISTYRKQLGLSQRQLAVQLGWIQSRLANYEANFRTPGLEEC
                     RKIVSTLNRLGAHCGLDDVFPPDGKHSENSIGAVDS"
     gene            3104..3523
                     /locus_tag="KW011_004272"
     CDS             3104..3523
                     /locus_tag="KW011_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH7662440.1"
                     /translation="MEKENEKIAASRLNDKIAMRLKERRQKLGLSQGKLAEICGWTQS
                     RIGNYEAGSRNVGVHDAVVLGKALGISPPELLFGEQESSELWLNESQRKLLELFNQLP
                     GSEQQRMIELFEVRLKEIDEYVEKYLRGRIKDNPPPE"
     gene            3829..3984
                     /locus_tag="KW011_004273"
     CDS             3829..3984
                     /locus_tag="KW011_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HBH7662441.1"
                     /translation="MNTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
BASE COUNT         1007 a         1080 c          894 g         1041 t
ORIGIN      
        1 ttcgctcttc cggcactcgc gacagattat attcaggcgc ctgtcgtagc ggcgtatttc
       61 tccgtctggt aatgaccaga taaggtcagg atcaaccaca accggtttct tcacctttac
      121 cctcgatagc tttttgcggg cgttttgcca gtctttatga gcctgttcag agggaaatac
      181 cccgtagcca gaattatata catcaccact tgcgaccagt tccatgcata agcgaccgac
      241 agacgcacga ctgatacctg tttcatctga caactgccga atcgtgaccc gaccgtcctg
      301 atgcacgagt tccacaattc gcgtcttcag ttcttcccgc tgttcgggag taaaaggttt
      361 cgccataaat cctcctgaaa ctacttaaca accctcgaat ggctaacatt cggacgccag
      421 ctctcccagt taaaattcac ccagcgcccg ccgttcatga ccatgcgatc catcacccgc
      481 tcgccgagaa gtgtattcat cgctgcatgg ttaatatttg tcagcatccc cacaccgcgt
      541 aacgatgccg tccggcgatc aacaatctgg ttcagtacca cctgctcgtt tttcgtatcc
      601 cgctgcatgc caatttcatc caggaccagc aggtcaacac cacaaagctc ctgtaaaaat
      661 ttttcaccgg atttgccgtt gtcgtagctc tcatgcaaca cgctcatgac atcggacacg
      721 gtgacgataa tcacgctacg ccccttcgcc atcagccggt tgccaatcgc gtctgcaaga
      781 tgattttttc cggtgccggg cttaccgctg aacacaaaat tcgtacagcc tgtcatcagt
      841 tcgtcagcta tggatttcgc ctgactcagt gcatgtcgct gaccgtcgtt ctgcgcccgg
      901 taattcgcaa acgaacactt ccggtgcaac ggctggatgc cggagcggtt caggattttt
      961 tccacccgca actgacgatt caggcggttg atctcctcgc aacgtttctg gccttcagca
     1021 agttgccact cgcgccactc cgcaaccgtt ctgaatgggg cggttacatg tggtggggtc
     1081 agtctgcgga tacgctccag aacgcctcct gtcgcaatat ttttcatggt ctgttacccc
     1141 ctgaagcctg gcgggatcgc actgtccggc aacgagacgg tgttaacctg tcggagcaac
     1201 gtctcaggcc gaacaccttt cggcgcgaac agaccctggt attcattggc gatgctgtgt
     1261 cgaatcacct gctcaggtgt aaaaccctgc tgacggaatt tttccagttc ccgtatcgcc
     1321 ccgttagcgc cctgctccgt tcgaatcggt tttcgcaatg cctgcctgaa ctggacccac
     1381 tcatgccaga gtgtttctgg caaccagtcg ggcagatcaa ctgacaacgg gtcaaatttt
     1441 ttctggcgaa cagccccacc gataaccaga attccctgct cggtgtgcct tgcgatatca
     1501 ctgcgccgca agacacatat cccggtttca acagcaccga ttttgagcag tgatttctgt
     1561 cgcggagaat ttcggtcaag ctcgctccca caccggacac cgttcctgtc agtcacgacg
     1621 atatcaatcc gccctccgca accgtcacca cgctctggca ccggatactc acgacagaca
     1681 tccaggccag cagcctgcaa cgcaaccacc gcagaattac aaaattccgc tgcagtaccg
     1741 ccagataatc tcccctcaag cactccagcc acaaaatccc gaaactccct tccggaaggg
     1801 aagggtttgg gatgggttag atctgttttt agatctttat ctgtatcttt attagttgcc
     1861 tttgtgttgg catcatgttc gaacatcact ccaacatctg tttgaacacc tgttaaattt
     1921 ctctcttgtt ttgtttgaac atctgcttcc tttctgcttc ttctggcctg aacagatgct
     1981 tttcctgcgg ctgatttttt ggttaatttt tctctgacag atgccagatc ttcctcaatc
     2041 cgaagatgca tccattcgtc gccgttatca caaaaaaact cccgcaagga tggttcaaca
     2101 tcaacccatc gctcgttagt cagacgggca atttttgcca acctgttttt gggtattggc
     2161 tttcctgttt gccagtaatt gaacatcagc aacaaatacg cgccgtgctc ctctgctgac
     2221 aaatgcatgg tgtcagccag gtaatcagct atgtacagtt gcatgtatgg taatgcggcc
     2281 ataattgccc cgtatgatgc tgcccggttg cttagaataa gcacaaacag catggaaact
     2341 tttgcttaat gaacaatgac agaatcgtcg gaagacccgc cgccgctgaa atgcgctttc
     2401 cggtaaacgg cctggactgc atcatcatgc gcatcaattg ccgtactcaa cgcttcctgc
     2461 gccgccagta atgcacggcg ttccagggta tcgaagatgc agagtcggtg acgcagctcg
     2521 cgcggaagaa ttgccagaac cgcagggatc agtttctgaa ttttttccct ttgcgctttc
     2581 gtttcacctt tcaaccaacg gtgatagata ttctgctgat tgttccagtc cttgcctggt
     2641 acaaggggca attcgccgcc cccctggcgc agatattctt cagtaattgc gttagcgacc
     2701 cacgcctgcc ctttttcggc tgccagggct aacaacactg attcgatgtg ctcatgcctg
     2761 attttcatga atcaaccgct cctatgctgt tttcgctatg cttaccgtct ggggggaata
     2821 catcgtcaag tccacaatga gcgccaagcc gattaagggt agaaacaatt tttctgcact
     2881 cctctagtcc aggggtacga aaatttgctt cgtaatttgc cagtcggctt tgtatccacc
     2941 ctaactgaac agcaagttgt ctttgagaca gcccaagctg ttttcgatat gttgaaattt
     3001 tgttcattga aaacctccga tgacaatttt aaacacacct tgtgttatat agccaagctg
     3061 ttttgtgttt tatgtaaatc acgattcgtg atacaaggat gcaatggaaa aagaaaacga
     3121 aaaaattgcc gctagtaggc tcaatgacaa aattgcaatg cgtcttaaag agcgcaggca
     3181 gaagcttggt ttatctcaag gaaaacttgc tgaaatctgc ggatggacgc aatcgcgtat
     3241 aggtaactat gaggcgggca gcagaaatgt tggagtacat gacgctgtcg tattgggaaa
     3301 ggcacttggc atatctcctc ctgagctcct ctttggagaa caggaatctt ctgaattgtg
     3361 gttaaatgaa tcccaacgaa aacttcttga gttgtttaac cagctaccgg gctcagaaca
     3421 acaacgaatg attgagctat ttgaagtccg gctaaaagaa atcgatgagt atgtagaaaa
     3481 atatttgaga ggcaggatta aagataatcc cccaccggaa taatgatctt gctatcacag
     3541 taatatgcca atcagcccgc tatcagcggg cttttttgta gcatcatcat atgacactca
     3601 ccataaaaca cattgcgtgt tgacacaaga aaacaaattg tgtttaataa gcatatccaa
     3661 acaacgcccc accagagaac ggcaggacaa tacctcgagt tatccagtca ctgaacaggg
     3721 ctaagtagcc agcctgaggc atacgaacat gacggcagtt gttgattgat acaaagcgca
     3781 gtagataaaa cgttccgcca cccggcgtta aggggaaatg gggtcaacat gaatactatc
     3841 gatcttggca acaacgaatc tctggtatgt ggcgtgtttc ccaatcaaga cggcacattc
     3901 accgccatga cgtataccaa aagcaaaacg tttaaaaccg aagctggcgc acgtcgttgg
     3961 ttgggaagac attcaggtga gtaaaatgaa cgagacagaa ttaaaacacg ttatcgctct
     4021 ac
//
LOCUS       DAESHI010000219         3961 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.222, whole genome shotgun sequence.
ACCESSION   DAESHI010000219 DAESHI010000000
VERSION     DAESHI010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.222"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..242)
                     /locus_tag="KW011_004274"
     CDS             complement(<1..242)
                     /locus_tag="KW011_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBH7662442.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(453..1850)
                     /gene="ccp"
                     /locus_tag="KW011_004275"
     CDS             complement(453..1850)
                     /gene="ccp"
                     /locus_tag="KW011_004275"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HBH7662443.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2255..3904
                     /locus_tag="KW011_004276"
     CDS             2255..3904
                     /locus_tag="KW011_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBH7662444.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1032 a         1001 c          928 g         1000 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggctta atttaaataa caaaatccta agcagaaaaa cgacttttta
      361 taatataaac atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg
      421 cccaaacatt cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      481 tacaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      541 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      601 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
      661 aatttatcac gctcttcttt agtaacattc atacgaccaa tatccgccgc cgtaatttcc
      721 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccacc gagaataata
      781 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
      841 gccgtcagcg cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
      901 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
      961 tagacgccga ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg
     1021 tcccaagatt tcgacgccat ttcaatcggg ttcaacggcg gtccaccagc ctgatcctgc
     1081 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
     1141 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
     1201 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
     1261 ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc
     1321 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
     1381 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1441 cccgcccagt gaagcgcggt ataacgcgtt ggcggcatag tttcatactg catcacccat
     1501 tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg
     1561 gcctcgaggt taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg
     1621 ccaggaatat aataataggc gggtaattct gccgaaggcg tgtggcaata gtcgcatcct
     1681 ttttcgcgga gaaaacctaa aaccttatta ttttcactga cagcggatgc ctgaacatcg
     1741 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     1801 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     1861 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     1921 agacaaagcc atcataaaaa acagtgagat acgctttttg ataggaatca atcaattaca
     1981 tgaatataaa ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc
     2041 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta
     2101 tttttataaa ttccatggat attagtgcgt tatatttttt gacaggttaa taaacgtgat
     2161 ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg
     2221 ataattagcg acgaatttcg gaggttggat ccttatgctc aatcagaaaa ttcaaaaccc
     2281 taatccagac gaactgatga tcgaagtcga tctctgctat gagctggacc cgtatgaatt
     2341 aaaactggat gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc
     2401 ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc
     2461 gaaaattttc ccggacagta aaacctttcc cgactgcgca cctaaaatgg acccgctgga
     2521 tattttaatc cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt
     2581 tgaaaatcac ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa
     2641 ttccctgaaa gagcatatcg accagctgtg gccggtgcta acccgcgaac cacaggatca
     2701 cattccgtgg tcttctctac tggcgctgcc gcagtcatat attgtcccag gcggccgttt
     2761 tagcgaaacc tactattggg actcctattt caccatgctg gggctggcgg aaagtggtcg
     2821 ggaagatttg ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actatggtca
     2881 catccccaac ggcaaccgca cctattattt gagccgatcg caaccaccgg tttttgcgct
     2941 gatggtggag ttgtttgaag aagatggtgt acgcggtgcg cgccgctatc tcgaccacct
     3001 taaaatggaa tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta
     3061 tcgccatgtt gtgcggatgc cggacggatc gctgctcaac cgttactggg acgatcgcga
     3121 cacgccgcgt gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc
     3181 gcccaacgag gtgtaccgcg atttacgcgc gggggcggcc tccggttggg attactcttc
     3241 ccgttggctg cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat
     3301 cgatctgaat gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa
     3361 aggcgagaaa gagacagaag cgctgttccg ccagaaggcc agtgcccgtc gcgatgcggt
     3421 aaaccgttac ctctgggatg atgaaaacgg catctaccgc gattacgact ggcgacgcga
     3481 acaactggcg ctgttttccg ctgccgccat tgtgccgctc tatgtcggca tggcgaacca
     3541 tgaacaggcc gatcgtctgg caaacgccgt acgcagccgg ttactgacac ctggcgggat
     3601 tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa cccaacggct gggcaccgtt
     3661 acaatggatg gcaattcagg gatttaaaat gtatggcgat gaccttctgg gtgatgaaat
     3721 cgcgcgcagc tggctgaaaa cggtgaatca gttctatctg gaacagcaca aaatgatcga
     3781 gaaataccat attgccgatg gtgttccccg cgaaggcggc ggtggcgagt atccgttgca
     3841 ggatgggttt ggctggacta acggtgtggt acgccgttta attggtttgt acggcgaacc
     3901 ataatatttt tacagccagc cgctaacttc ctgctggctg taaaattatc ctcttcagga
     3961 g
//
LOCUS       DAESHI010000220         3945 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.223, whole genome shotgun sequence.
ACCESSION   DAESHI010000220 DAESHI010000000
VERSION     DAESHI010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.223"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            30..968
                     /locus_tag="KW011_004277"
     CDS             30..968
                     /locus_tag="KW011_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HBH7662445.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDNPPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            1024..2313
                     /locus_tag="KW011_004278"
                     /pseudo
     CDS             1024..2313
                     /locus_tag="KW011_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            2310..2603
                     /locus_tag="KW011_004279"
     CDS             2310..2603
                     /locus_tag="KW011_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBH7662446.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            2660..3945
                     /gene="fadK"
                     /locus_tag="KW011_004280"
                     /pseudo
     CDS             2660..3945
                     /gene="fadK"
                     /locus_tag="KW011_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
BASE COUNT          930 a         1003 c         1087 g          925 t
ORIGIN      
        1 cctgaactga agcctacgga gaagaaacga tgagtcaatt aaacagcgtc tgggtcttta
       61 gcgataatcc tgaacgttat gctgaactgt ttggcggcgc gcagcaatgg ggccaacagg
      121 tgtatgccat tgtacaaaat accgaccagg cgcaggcagt tatgccttat ggtccaaaat
      181 gtatttatgt tcttgagcaa aatgatgcgc tgcaacgtac tgaaaattac gccgaaagca
      241 ttgctgccct gctgaaagat aacccccccg ctatgttgct gctggccgcg acgaaacgtg
      301 gtaaagcgct ggcagcacgg ttaagtgtgc aactgaatgc ggcgctggtg aacgatgcca
      361 cggcagtgga tattgtcgat ggtcacattt gcgccgaaca ccggatgtat ggcgggttag
      421 cgttcgctca ggaaaagatc aacagcccgc tggcgatcat tacccttgca cccggtgttc
      481 aggaaccgtg caccagtgat acctctcatc agtgcccgac agaaacggta ccttatgttg
      541 ccccgcgtca tgaaattctc tgtcgcgaac gccgtgcgaa agcggcaagc agcgtggacc
      601 tgagcaaagc aaaacgtgtg gttggcgtcg gtcgtggtct ggcagcgcag gatgacctga
      661 aaatggtcca cgaactggcg gcggtgctga atgctgaagt cggctgttca cgtccaattg
      721 ccgaaggcga gaactggatg gagcgtgaac gttatatcgg tgtctccggc gtgttgctga
      781 aatccgatct ctacctgacg ctggggatct ccgggcagat ccagcatatg gttggcggca
      841 acggcgcaaa agtgattgtc gccatcaata aagataaaaa tgcgccaatc ttcaattatg
      901 ccgactacgg tctggtgggc gatatctaca aagtcgtccc tgccctgatt agccagttga
      961 gccgctaatt cccctttcaa ccacatctcc cgctgtaaac gcagcgggaa gggagcataa
     1021 cgcatgtcgg atgacaaatt tgatgccatt gtggtcggtg cgggcgttgc tggtagcgtt
     1081 gccgcactgg tcatggcgcg agccgggctg gatgtcctgg tgatagaacg cggcgacagt
     1141 gccggatgta aaaacatgac cggcgggcgt ctttatgccc acacacttga agcaatcatt
     1201 ccaggctttg cagtatcagc gccggtagaa cgcaaggtca cacgcgagaa aatctccttc
     1261 ttaaccgaag aaagcgccgt taccctcgat tttcaccgcg agcagccaga tgttccgcaa
     1321 cacgcatctt ataccgtatt gcgtaatcgt ctggacccgt ggttgatgga acaagccgag
     1381 caggcgggcg cgcagtttat cccaggcgtt cgcgtcgatg cgctggttcg tgaaggaaac
     1441 aaggtaactg gcgtgcaggc tggggatgat attctcgaag cgaatgtggt gattctggct
     1501 gatggcgtta actcaatgct tggccgctcg ctgggaatgg ttcccgcttc cgatccgcat
     1561 cattacgctg ttggtgttaa agaggttatt ggcctcacac cagaacagat caacgatcgc
     1621 tttaatatta cgggcgagga aggtgccgcc tagctgtttg ccggttcccc ttctgacggc
     1681 ctgatgggtg gcggattcct ctataccaac aacgattccg tatcgttggg gctggtttgt
     1741 ggattgggtg atatcgccca tgcgcaaaaa agcgtgccgc aaatgctgga agattttaaa
     1801 caacaccccg ccattcgccc actaattagc ggcggcaaac tgcttgaata ttccgcgcat
     1861 atggtgccgg aaggcggtct ggcgatggtg ccgcaactgg ttaacgaggg cgtgatgatc
     1921 gttggtgacg ccgcaggctt ctgtctgaat ttgggtttta cggtccgcgg catggattta
     1981 gccattgcat cggctcaggc tgccgccaca acggtgatcg ccgccaaaga acgcgaggat
     2041 ttctccgcca gcagtctggc gcaatacaag cgtgagctgg aacaaagctg cgtcatgcgt
     2101 gatatgcagc attttcgcaa gatcccggcg ctgatggaaa acccgcgcct gtttagccaa
     2161 tacccacgaa tggtagccga catcatgaac gagatgttca ccatcgacgg caaaccaaac
     2221 cagccggtac gaaaaatgat catgggacat gcgaagaaaa ttgggctgat caacttgctg
     2281 aaagatggca ttaagggagc aaccgcgcta tgagccagaa cgctacggtt aacgtcgaca
     2341 tcaaattagg cgtcaataaa ttccatgttg atgagggcca cccgcatatc attctggcgg
     2401 caaatcccga catcaatgaa ttccgtaaat taatgaaagc ctgccctgcc ggactttata
     2461 agcaggatga cgccggcaac attcattttg attctgccgg ttgtctggag tgcggcacct
     2521 gtcgggtgct gtgcggtaac actattctcg aacagtggca atatcccgca ggcaccttcg
     2581 gtattgattt tcgctacggc taatcatgca tcccacaggc ccgcatctcg ggcctgatgt
     2641 tctgtttcga gagtccaaca tgaaagtgac attaacgttc aacgaacaac gtcgtgcggc
     2701 gtatcgtcag caagggctat ggggcgatgc ttcgctggcc gattactggc agcagaccgc
     2761 tcgtgcgatg ccagacaaaa ttgccgtggt cgataatcat ggtgcatcgt acacctatag
     2821 cgcgctcgat cacgccgcga gctgtctggc aaactggatg ttagcgaagg gtattgaatc
     2881 aggcgatcgc atcgcatttc aactgccagg ctggtgtgaa tttaccgtta tctatcttgc
     2941 ctgcctgaaa accggtgcgg tttccgtgcc gctgttgcct tcctggcggg aagcagaact
     3001 ggtgtgggtg ctcaataagt gtcaggcaaa aatattcttt gcaccgacgt tgtttaaaca
     3061 aacgcgtccg gtagatttaa tcctgccgct gcaaaatcag cttccacaac tacaacaaat
     3121 tgtcggcgtg gacaaactgg ctcccgccac ctcttccctc tcattaagtc agattatcgc
     3181 cgacaatatc ccactgacca cggcgataac gacccacggc gatgaattag ctgcggtgct
     3241 gtttacctcc ggaaccgagg gtctgccaaa gggcgtaatg ctaacgcata acaatattct
     3301 cgccagtgag cgggcttatt gcgcgcggct gaatctgacc tggcaggatg tctttatgat
     3361 gcctgcgcca cttggtcacg caacgggatt tctgcatggc gtaacagcac catttttaat
     3421 tggtgctcgc agcgtgttgt tagatatttt cactcctgat gcatgtctcg cgctgcttga
     3481 gcagcagcgt tgcacctgta tgctcggcgc aacgccgttt gtctatgatc ttttgaattt
     3541 actagagaaa caacccgcag acctttcagc gctgcgcttc tttctttgcg gcggtaccac
     3601 aatccccaaa aaagtggcgc gcgaatgcca gcagcgctgg cattaaatta ttaagtgttt
     3661 atggttccac agaaagttcg ccgcatgcgg tggtgaatct cgatgatcct ttgtcgcgct
     3721 ttatgcacac cgatggttac gctgccgcag gtgtagagat taaagtggtc gatgacgcac
     3781 gcaagacctt accgccaggt tgcgaaggtg aagaagcctc gcgtggcccc aatgtgttta
     3841 tggggtattt tgatgaacct gaattaaccg cccgtgcgct ggatgaagaa ggctggtatt
     3901 acagcggcga tctctgccgt atggatgagg ctggctatat aaaaa
//
LOCUS       DAESHI010000221         3936 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.224, whole genome shotgun sequence.
ACCESSION   DAESHI010000221 DAESHI010000000
VERSION     DAESHI010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.224"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..585
                     /gene="fimZ"
                     /locus_tag="KW011_004281"
     CDS             <1..585
                     /gene="fimZ"
                     /locus_tag="KW011_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HBH7662447.1"
                     /translation="MSIEVLLQKNSELQIVLKTDDYRITIDYLRTRPVDLIIMDIDLP
                     GTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANGFVSKCNDQNDIFHPVQ
                     MILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRYLVSGLSNKEIADKLLL
                     SNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(588..1103)
                     /gene="sfmF"
                     /locus_tag="KW011_004282"
     CDS             complement(588..1103)
                     /gene="sfmF"
                     /locus_tag="KW011_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HBH7662448.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVE
                     KADAIFMINYN"
     gene            complement(1114..2121)
                     /gene="fimH"
                     /locus_tag="KW011_004283"
     CDS             complement(1114..2121)
                     /gene="fimH"
                     /locus_tag="KW011_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HBH7662449.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(2134..3936)
                     /gene="fimD"
                     /locus_tag="KW011_004284"
                     /pseudo
     CDS             complement(2134..3936)
                     /gene="fimD"
                     /locus_tag="KW011_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
BASE COUNT         1094 a          971 c          861 g         1010 t
ORIGIN      
        1 atgtctattg aagttctgtt gcaaaaaaac agtgaattgc agattgtcct gaaaacggat
       61 gattaccgca taaccatcga ttatctccga acccgtcctg ttgatttaat cattatggat
      121 atagacttgc ccggaacaga cggttttacc ttcctgaaaa ggatcaaaca aatccagagc
      181 acagtgaaag tgttattttt atcatcgaaa tcagaatgct tttatgctgg cagagcgata
      241 caagctggtg ctaacggttt tgtcagtaaa tgcaatgatc agaatgatat ttttcatccc
      301 gttcagatga tcctctccgg atacacgttt tttcctagcg aaacgctcaa ctatataaaa
      361 agcaataaat gtagtacgaa tagttcaacg gtcactgtgc tatctaatcg tgaagtgacc
      421 atattacgtt atctggttag cggattatct aataaagaaa ttgccgataa gttattactt
      481 agcaataaaa cagttagtgc gcataaatct aatatttatg gcaaactagg tttgcattca
      541 attgtagagc ttatcgacta cgccaaatta tacgaattaa tataatatta attataattg
      601 atcataaata tcgcatccgc tttctccaca cctggctgaa caccgctggc taacgctcga
      661 taattggcaa aaaagtttag tgtgacattg ccatttgcat ctacttcctc agtcgggctc
      721 gcctccccca gtgcgagccg ggagcgatcg ctattacgta attcgatggc gacggtttgt
      781 gccattgcgg gatcatccag agccagcagg ttggtatcgg atgccggcgt tcccgtaaat
      841 aaaatcgcaa ctgaacccgg aggacatccc tccagccgca ggctaaaagg gacgagtgcc
      901 gtggtatcgc cagcgttcag taattgtgtc gtaggccatc tgcctaaatc taccgtctta
      961 tcaatatccg cagtgtttac ggtacaggag aaatcaacaa cgttaccgtg caaattgata
     1021 ttaatcgttc ccaaagggtc agctgcccat ccactggaac tccacagtaa cccgcagaaa
     1081 cagctaaaga gtactcttct cattctccgt acctcattga taatcgacgc gtaaataccc
     1141 cagcgcgcta aacggccctt cggtcggttt ttgaccggta atactgatag gccaggcgcg
     1201 aagtgtgaca ttggctgccg cagctgcatc cagacggaaa ggaataacgc tattgagatc
     1261 gttaggcgtg atcggcgtat cgttctgatc ggcgacaata aaacctaaat cctgattgtc
     1321 cgacaccatc gcctgaccag aaacggcact ggcttccaga cgcattgtta aataagcctg
     1381 cgcagcaaca ttcgtacatt tgatcgcaat gctcttggtt tgcggcatga cgccagctgg
     1441 tcgattcccc ggccctgccg cactaaataa cgatgcgccg atatcaccaa aatcaaattc
     1501 aacaatctgc ccggcattta attcgcagtt ttgcggcact tcaacccggc caccaaaact
     1561 aatggtataa acaggtgtaa ccaacggatc gtaaggcgtt gatgtcacat acacggtaaa
     1621 catcacctgt ctggggatct ccaccatgtt aataaaggga cgaataacct ttaaacgaaa
     1681 tatcaaacga gaatccatca ccccgaacgg aaggttttgc gaaacgttag gatctgttcc
     1741 catccgaata taattttgcg gggggaactg aatatccatc acactatcaa ccagactcat
     1801 cgcacctaat agataatcat ttaattgcat atatttataa ttatcgatag tttcctgaac
     1861 aataaagtta gtgacataac tacggtatgt ataattcacc agcgtgccgg gtggacaaat
     1921 tgctgagaca cctacccagc cggatttttc cggtaaaaca acaatctgcc ctggctggtt
     1981 attggtgcta ttaaagacat ttgagagatc atagtgttca tcttctgcta cgccagtact
     2041 atttgcacaa acagtagccc aggatatgtt tgccagacac aaacatgcca ttgccaataa
     2101 taatctgcag attatcttca ttgataattt cctttatgag ccaggatgtg tgcaaactgc
     2161 cgaaataaca gtgacggctt gctgtaagct ttgctttggc aatacatagt gcgcaatgca
     2221 gcgtgagttc gcgccgtctc cccactgaac cagtaattca ccagacaatg gcgcaccact
     2281 taaataaact tgcccgtcat cacccaccat actggttatt ccggtactgt tttcccgtac
     2341 cagtgatcca aacggcaccg gttttccgcc ctgggtaacg gtaatgagcg cccgcacgcc
     2401 tatgcgggta tcaaaattgg cacgaaccaa cgcgccttgc gtcggcacaa cgctgctaat
     2461 atttttttca acatcgatgg aattccccat cgtattggta tcaagcgcga tacggttata
     2521 ccgataaacc gtggcatacg gcatcaccgc atagccgcgc cagtcggtta aaatgccagt
     2581 ttgattttca atgcgtacac cgcctgcgcc aggcgcttta atcaaaacat tggtatcccc
     2641 taaaggctgg ctcagtgtta tgccattttc atgcccgacc acaccgccag acagctgcca
     2701 gttaacgtca tgttgatcgc ggtcgtagtt atacccgacc cccagcgtac cgtaagtggc
     2761 ctgccagttt gccgtggcgc tgcccgtgta cccattattc gaggtatcgc cctggcttac
     2821 gtgataactc aggttgtggc cttccagtaa ggtcccacca acgcctgcca gccagctatt
     2881 ttgcccgttg ctgttacggt tagcgttcaa ggaggcataa gcgcggtcga gcgcattttc
     2941 ccgggtgtag cgacgtttgg tcaaaacatt gaaaggcact gaaacattaa gtccgacaat
     3001 atgttcgtta tcggggatcc ctacagattc attccacgaa aatgagagtg aataactgat
     3061 gccaacccag ctgctggtat accccacctg ataccaggta tctgaatccg aagtattcca
     3121 gtatttttga tgggtaccag agatataaag cgagccaaag tcattaagtg attgtgaaat
     3181 atttaactgc aaacggtctt tgcggctaaa gcgtaaattg tggtaattca cgattatcgg
     3241 ttcatcgcga tgctcaccgt cgtaatcgta atcatattcg tacccctcca ttcgacgata
     3301 cgcaacatca tcaagggtat aaaaaccttg tgtcgaatag cggtaaccca ttaactgaaa
     3361 attggtgccg aaggtgtaca tcgatttcgc atagaggaag cgaatggaat ccccctcatg
     3421 acgactgtcg tcggctaact ggctgcgcgc atgggtcaca tccagcgaca ctgcgcccca
     3481 gttcccgagg ttgcgcccca gccctaataa aaaggcggtg taattggcag ataattgcgt
     3541 cccgccgtag gcagtaaatt cctgtggtaa accaccgagt gccgtgccct gaaaaaagaa
     3601 aggtgatgat tgctgactat taccgctacg aaaatcgccc gccgtcaggt caaatttgaa
     3661 acgcccttcg cgttgtaaaa tcggcactgt tgaatacgga attgtgtaat tctgccatcg
     3721 cgctcgtcga tggtgacatc cagatcgcca tttgaagatg tcgggtgcaa atcagtaatt
     3781 tcaaaagcac cgggggaaac atagctttga tagataataa aaccattttg ccgaatcgtt
     3841 agctgggccg ccgtacgggc aatcccacgt accgttgggg caaacccttg ctggctatct
     3901 ggatacatat tatcggaaga ataaagccgt acacca
//
LOCUS       DAESHI010000222         3923 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.225, whole genome shotgun sequence.
ACCESSION   DAESHI010000222 DAESHI010000000
VERSION     DAESHI010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.225"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..301
                     /locus_tag="KW011_004285"
     CDS             <1..301
                     /locus_tag="KW011_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662450.1"
                     /translation="RVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSN
                     NRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
     gene            390..2258
                     /locus_tag="KW011_004286"
     CDS             390..2258
                     /locus_tag="KW011_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HBH7662451.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDI
                     KFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKNLLNGKYFDSGYIPCSRADES
                     IQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQTILRKVTFSNIES"
     gene            2295..3923
                     /locus_tag="KW011_004287"
                     /pseudo
     CDS             2295..3923
                     /locus_tag="KW011_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
BASE COUNT         1232 a          721 c          769 g         1201 t
ORIGIN      
        1 acgtgtagaa ctgtattacc gctttaacca tgaagaagaa tattaccctt ctcaccgttt
       61 tatgcttgtt gttgatatga agacagtaat ggttttttca caacgagctg aatcactggg
      121 gttatactcg aataatcgct tgcttatcaa tataattgcc gaacatatcc atagtgatat
      181 atatctaacc aagtatcagc aacaatatcc agatgccaat gcattaataa atactttgca
      241 tgaatctggc aatgatatgg tgcaggatga aaaacatttg agtgatagca aaaaccgtta
      301 atattttcct ttcattgtga tcgtctgtag gcgatcacaa tctatttgtc ttttataacg
      361 tctaaacttt tttatgcaaa ggtgtattta tgattttttt taacgatata aagcccactg
      421 gttggttaaa gaataagatg gaatggtata tgaacggtca tataggagaa ctggataaat
      481 tagttcccca tcttattacg gagcataaaa tatatggaag agatcgtata actccacgca
      541 ctgtatctgc tgaagatggc gttattaaaa aacagaataa tcccgatgat aacatgatgc
      601 aatattattg gtgggatagt gaaactcaat cgaattggaa ggatggatat tgccgcagtg
      661 cctacttgtt agataataaa gaatggcagg aaaaagcgag cgatttatgt ttggaactta
      721 tcgatacgca agatgatgat ggctacattg gaatttatga ttcatcatta aaatttaact
      781 gtaaagcaga aaacggagag ttctgggcac aagctactgc cttgcgtttt gtgcttggat
      841 gttatgagtc cacccgtaat gagagtttat taacttctct tacccaagcc gcacaatgca
      901 ttatggctgg ctaccctaaa gaaacgtcac atcctttcgt tgtggaacag ggttttgctg
      961 gacatagcca cggattaaca ataacagatg ttttctaccg cctttatgaa ataaccaata
     1021 acattgaata tctctcatat tgcttgtggc tgtatgaaga ttattccgca ggatgcgtgt
     1081 cggagcaaga tctgcaatat aaaaatgtca tagaccctgg ttaccttctt aaaggtcatg
     1141 gcgtacatac gtatgagcat attagggcat tagccatcgc agcgagccaa cggccgcagt
     1201 atcaaactgc attagatatt tttttatccc agttgaaata ttatcttaca ccatctggcg
     1261 ctcccattgg tgatgagtgg attacaggac gcacagctaa tgccacttat acaggctatg
     1321 aatattgttc tgttcatgaa ctatttagca cttatgttat gttaataaaa ctgaccacta
     1381 atattggtct ggccgatgat gctgagtggc tattctataa tgctagtttt ggcatgcagc
     1441 atcccatact tcacgcgata atgtactgta agactgataa ttgttatgaa gcaaatgaaa
     1501 ggaaacatcc tgacagtaat cagtttaata ccagatataa atactcccct actcatgagg
     1561 aggctgctgt ctgctgcgta ccgaatacag caagaaccat accggctttt gtcgaaaata
     1621 tgtttcaaga aaataacaat ggttttactg cattatttta tggcccgtgt gagttttatg
     1681 gcacatttaa taatgttgca attaaaatta cacaattaac tgaataccct catgatttaa
     1741 gcgtaaaatg tctcattgag cctgagtcat ctgttcgttt tgctctcagt ttccgttacc
     1801 ccggctgggc aaaaatgatg attattaatg gtgttacttt tactaaaaat gatactcaaa
     1861 actcacttat tattttagat agaacatggc aacatcatga tgtgattgac attaaattta
     1921 tagctgatat acagtttaat actgacttat gtggtgacac ttatatttcc agaggtcccg
     1981 ttttatatgc aattgagctt gagagcgata ttttaatcaa aaaaaacctg ctgaacggga
     2041 aatattttga ctcaggatac ataccctgta gtagagctga tgaatctatc cagttctctt
     2101 atgctgatat ggaatctttt tcatattcaa cctcaccaga gggcaaacaa tacattgaag
     2161 gctgttttta tttaaacaag actaaaatag tcagaaaact tataccattt ggtcaaacca
     2221 ttcttcgcaa agtaactttc tccaatatag agagttaata ttttcgagtg ttataattaa
     2281 ttaaagggat ttctatgtac cttactgata atattatcaa actgtcgaat aaaataggtc
     2341 agcgtgttta tactgttgtc tctgaaatgt cgattgaagc atggataaca aaagaaccgc
     2401 agccttttgt ttatcgccgt agcggcgctt accagaaact caccattggc tcagactggg
     2461 gtcaactgtt tgactgtgcc tggatgcgag tttatggtaa acgtccagca gataaattta
     2521 gacacaaact cgtcgctctc gttgatatcg gtggcgaagg tcttattgtg gataagaatg
     2581 gttcacccat ctgcggcatc acgaataaag catcatctta tggcgtaccg ccagataaac
     2641 caggtaaatg ggtggttgac ctttctctcg ttagtgaaaa tgacgaggtt gagttttgga
     2701 ttgatgctgc ctgcaacgat ttatttggct atgttactaa tggagggatt attactgatg
     2761 ttcatatcgc gacacgtaat caactactga aatcccttta ttatgatatt gaggtgcttt
     2821 tcgactggat taatgatggt cagaagtttg aatccataca tcctaaaggt atcaccccag
     2881 aacaatttat tgccaaaaga agtgagcgta ctgacgaaat cataagtact ctcgaatata
     2941 ttgataatac gttaattacc tttagcaatg aagaaataat taaatgtcaa attgcaatcc
     3001 aatccataat caataaaaac aataatcctt cagagttcag gattatggct actggtcatg
     3061 cccatttaga tatagcgtgg atgtggcctt taagagaagg acgcagaaaa gcaataagaa
     3121 catttgccac cgcccttgca aacattgaaa aatatcctga ttacattttt ggcgctagtc
     3181 agtaccaact ttttcactgg ataaaaaaag attatcccta tttcttcgaa aagttaaaaa
     3241 aacaaatcgc cgcaggccgc tttgagttac aaggttgctt ctgggtcgaa tgcgatttaa
     3301 atctggtaag tggtgaatcg ctgataaggc aaatcatgca tgggacacga ttcacactgc
     3361 agaatttttc aaaaaagata aattatgtct ggcaaccgga tgtctttgga ttccccgcaa
     3421 cgcttccaca aattctcaaa aaatcaggca ttaactatat tgcatcgcaa aagctctcac
     3481 aaaacaagat taataaattt aataattacc ttttccgctg gcagggcctg gatggctcag
     3541 aaattctgat gcataacttt cctgaagaca cttatgatag ccgagcacgt gctcgctctt
     3601 tagaatatat tgaacaaaac tataatgaaa aagagatctg tccctatgcg cttatggtgt
     3661 atggtgtatg gtgtcggtga tggcggcgct ggccccggtg aggaacatat agaacgatta
     3721 acacgtatac gcaatattga tggcttacct catgttgatt tcagccgtgt tgataagttc
     3781 ttcacttacg ccgacgcctt tcgcgaaagc ttgcccatta tttcaggtga actttatttc
     3841 gaggctcatc aagggtgctt tacatcagag tcagcaacta aagctcataa tcgaaatatg
     3901 gaaaataaat tacatgatgc tga
//
LOCUS       DAESHI010000223         3908 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.226, whole genome shotgun sequence.
ACCESSION   DAESHI010000223 DAESHI010000000
VERSION     DAESHI010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.226"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            24..590
                     /locus_tag="KW011_004288"
     CDS             24..590
                     /locus_tag="KW011_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HBH7662452.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            594..1169
                     /locus_tag="KW011_004289"
     CDS             594..1169
                     /locus_tag="KW011_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HBH7662453.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1211..2263)
                     /locus_tag="KW011_004290"
     CDS             complement(1211..2263)
                     /locus_tag="KW011_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBH7662454.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRCRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2488..3408
                     /gene="lpxL"
                     /locus_tag="KW011_004291"
     CDS             2488..3408
                     /gene="lpxL"
                     /locus_tag="KW011_004291"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HBH7662455.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3580..3777
                     /locus_tag="KW011_004292"
     CDS             3580..3777
                     /locus_tag="KW011_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBH7662456.1"
                     /translation="MSPCGNDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIIPS"
BASE COUNT          972 a          987 c          951 g          998 t
ORIGIN      
        1 gcaaattcgt attggatgca cttatgtcat tcacaaatac ccctgaacgc tatggtgtta
       61 tatctgcggc cttccattgg ctaagcgcaa ttattgtcta cggcatgttt gctttaggtc
      121 tgtggatggt cacgctcagt tattacgatg gctggtatca caaagcaccc gaactgcata
      181 aaagcatcgg tattttgtta atgatggggc tggttattcg cgttctgtgg cgtgtcatat
      241 ctccaccgcc gggtccgctg ccgagttatt cgcccatgac tcgtcttgcc gcaaaggctg
      301 gacatctcgc attgtaccta ttgctgttcg ccatcggcat cagcggctat ctgatctcaa
      361 ctgccgatgg taaaccgatc agcgtttttg gctggtttga cgtccccgcg acccttgccg
      421 acgctggcgc acaggcagac tttgccggtg ccctgcattt ttggctcgcg tggagcgtcg
      481 tagtactgtc cgttatgcac ggatttatgg ccctgaagca tcatttcatc gataaagacg
      541 acactctgaa gcgcatgctg ggaaagtcgt catctgacta tggagtataa aaaatgaaaa
      601 aaagcctgct tggtttaacc ttcgcgtccc tgatgttctc tgccggttca gcggttgccg
      661 ccgattacaa aattgacaaa gaaggtcagc acgcctttgt taatttccgc atccagcacc
      721 ttggctatag ctggttatac ggcaccttta aagatttcga cggtactttt acctttgacg
      781 aaaaaaatcc ggctgccgat aaagtgaatg tgacaattaa caccaccagc gtcgatacta
      841 atcacgccga acgcgataaa catcttcgca gtgcagattt cctcaatacc gcaaaatatc
      901 cacaggcaac attcacctcc accagcgtga agaaagacgg tgacgaactg gatattaccg
      961 gcgatctgac gctgaatggc gtaaccaaac ccgtcacgct ggaagcgaaa ttaattggtc
     1021 agggcgacga cccatggggt ggtaaacgtg caggcttcga ggccgaaggc aaaattaagc
     1081 tcaaggactt caatatcaag acagatttag gtccagcttc tcaggaagtg gatctgatta
     1141 tttcagtgga aggcgtacag cagaagtaag cgtgaaatgt tgccggatgg cacgcatccg
     1201 gcaatgatat ttattctgtt ggatcaggaa tgcccagtgt tgtattcaga cgtccacgag
     1261 acttattaaa gatcttattg ccattttcac gtcctgcccg acggcatcgc tgttcttctg
     1321 gcggtaacgc gctttcttcg cagcaaatct cactacaaca acctttgtat ttttccgcgc
     1381 atactggaca ctgaataaac agcaggtggc agccatcatt tttacagttg gtatggctgt
     1441 cgcacggcgc gccgcactgg tggcaatgcg cgataatctc atccgatata cgttcgccca
     1501 tccgctcgtc aaaaacaaaa tttttgccaa taaaacgcac cggcaagccc tgctcgcgcg
     1561 ccttacgggc gtattcaata attccgccct cgatatgcca gactttgttg aatccgttat
     1621 gtttcatcca ggcactggcc ttttcacaac gaatgccgcc ggtgcagtac atgacgattt
     1681 ttttatcttt atgtgcctgc atcatctcga ctgcttttgg cagctgctca cggaaggtat
     1741 ctgccggaat ttcgagcgcg ttttcaaagt gccccacttc atactcatag tggttacgca
     1801 tgtcgataaa tagtgcatcg ggatcgtcaa gcatggcgtt cacttccgcc gcttgcagat
     1861 actcaccaac attgctggca tcaaagtgag gatcgtcaat accgtcggca acaatgcgat
     1921 cgcgtacctt catgcgcagt acccagaagg atttcccgtc atcatccaac gcgatattca
     1981 ggcgtaaacc ctctaaagcc gggtcgaagg catagagctg cgcgcgaaat gtttcaacat
     2041 tgctcgcagg tacgctgatt tgcgcgttaa tgccctcatg cgccagatac actcgcccaa
     2101 aaacattcag cgcggtaaac agctgatata aagcgtcacg ggtcgcctta ggatcggcga
     2161 tgtggaaata cttgtaaaac gaaatggtgg ttcgcggttc gctctcaacc aacattttgg
     2221 cttttaacgc gtcgttggaa atgcggttgt gtaacactgg catggtgtac ggttcctgcg
     2281 agatgggaaa gtaaaaatcc gcggcatgat atagcaatta tcgataatta acatccacac
     2341 attttacgct acatttgcgc attaaaaatt atttgttatt tacaagcgcg gcaatttcgc
     2401 ccagtcttca gccacaaatt ttggttgcgg gcgaaaaaat gcgacaatac atacaattgc
     2461 ccgaataggt tgaaaaacag gattgatatg acgaatctac ccaagttctc caccgcactg
     2521 cttcatccgc gttattggtt aacctggttg ggtattggcg tactttggtt agtcgtgcaa
     2581 ttgccctacc cggttatcta ccgcctcggt tgtggattag gaaaactggc gttacgtttt
     2641 atgaaacgac gcgcaaaaat tgtgcatcgc aacctggaac tgtgcttccc ggaaatgagc
     2701 gaacaagaac gccgtaaaat ggtggtgaag aatttcgaat ccgttggcat gggactgatg
     2761 gaaaccggta tggcgtggtt ctggccggac cgccgaatcg cccgctggac ggaagtgatc
     2821 ggcatggaac acattcgtga cgtgcaggcg caaaaacgcg gcatcctgtt agttggcatc
     2881 cattttctga cactggaact gggcgcgcgg cagtttggta tgcaggaacc ggggattggc
     2941 gtttaccgcc cgaacgataa tccactgatt gactggctac aaacctgggg ccgtttgcgc
     3001 tcaaataaat cgatgctcga ccgcaaagat ttaaaaggca tgattaaagc cctgaaaaaa
     3061 ggcgaagtgg tctggtacgc accggatcat gattacggcc cgcgctcaag cgttttcgtc
     3121 ccgttatttg ccgttgagca ggctgcgacc acgaccggaa cctggatgct ggcacggatg
     3181 tccggcgcat gtctggtgcc cttcgttcca cgccgtaagc cagatggcaa agggtatcaa
     3241 ttgattatgc tgccgccaga gtgttctccg ccactggatg atgccgaaac taccgccgcg
     3301 tggatgaaca aagtggtcga aaaatgcatc atgatggcac cagagcagta tatgtggtta
     3361 caccgtcgct ttaaaacacg cccggaaggc gttccttcac gctattaaat ctcccctgcc
     3421 ggatgcttca gaatggcatc cggcattacc acagcaaatc cccctgattt agcgataaaa
     3481 gctctctgga ttgcgccccc tggaagtcgg gcgcataatt agtgtgctta tcttttcttc
     3541 ttatgttcac cgcgcctggc gcaccaacag cggattgcta tgtcaccctg tggaaatgac
     3601 acccctataa actggaaacg aaacctgatc gtcgcctggc taggctgttt tcttaccggt
     3661 gccgccttca gtctggtaat gcccttctta cccctctacg ttgagcagct tggcgttacc
     3721 ggtcactccg ccctgaatat gtggtccggt attgtcttca gtataatacc ttcctaaata
     3781 cactacctga gagaagagat gaaaagagct atcagctaca tgaggttttc aactgagaag
     3841 cagataaaag gggactctat acgaagacag tctaagctgg tcactgactg gttatcaaag
     3901 aaccctga
//
LOCUS       DAESHI010000224         3852 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.227, whole genome shotgun sequence.
ACCESSION   DAESHI010000224 DAESHI010000000
VERSION     DAESHI010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.227"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            187..294
                     /locus_tag="KW011_004293"
     CDS             187..294
                     /locus_tag="KW011_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBH7662457.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(381..2060)
                     /gene="bcsG"
                     /locus_tag="KW011_004294"
     CDS             complement(381..2060)
                     /gene="bcsG"
                     /locus_tag="KW011_004294"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HBH7662458.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2057..2248)
                     /gene="bcsF"
                     /locus_tag="KW011_004295"
     CDS             complement(2057..2248)
                     /gene="bcsF"
                     /locus_tag="KW011_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HBH7662459.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2245..3816)
                     /gene="bcsE"
                     /locus_tag="KW011_004296"
     CDS             complement(2245..3816)
                     /gene="bcsE"
                     /locus_tag="KW011_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HBH7662460.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT          982 a          967 c          993 g          910 t
ORIGIN      
        1 caagccggaa aggttccggt gaggcgcaat gttgcggggg cttaatccct ggtggcattg
       61 gttgctggag agagaaaacc cccgcacgtt gcaggtatgc acctgacaac atcacggggg
      121 ctaatcttga acccaaacaa ctcaagaata gccgcgaaac gttggcatta caacacaggc
      181 ggctatatga cgctcgcaga actgggcatg gccttctggc atgatttagc ggctccggtc
      241 attgctggca ttcttgccag tatgatcgtg aactggctga ataagcggaa gtaacgtgtc
      301 atgcgggcgt caggctgccg taatggcaat ttgcgcccgg atcaggccgc agaggttaaa
      361 ctctgcggcc tttttcgtgc ttactgcggg taaggcaccc agtcgccgcc gttcaggcga
      421 acgtacggtt tatcctggta ttgaataact actgcatttg agttctcgga gaccggtgct
      481 gtttgtggca acccactggt gagttttttc cagtcaacat tgtcttcggt gaaaatcttg
      541 ccatcaagaa cgcgaaccac cagatcggag atagctagga agctgctcgg ttggtcaatg
      601 acaatcggtg ccccctgatg tggtgccttc atgccgaaga atttcacccc aacggggacg
      661 tctgtgatag acgggctagg gatatcacgt aggccagata cctgcattct gtcgcccttc
      721 agcgcgccgc cgtgttccgg caccacgacc accatcactt tacgacccga tttctccagt
      781 tcagtaaaga aggcgtccag ttcatcaaag aatttctgcg cccgcgcttt gtaatccgct
      841 gttttgctga cccccggata atggttgccg tcatgcagtg gaagcgtgtt gtagaacgtg
      901 gcactacggc tgtttttatc tttttcggta acgtccagcc agcggttaag cacggcggta
      961 tcgtcataaa ccggcgaacc atcaaagccc agcaaaataa ccggcagatt tgtttgatcc
     1021 atcaattcag tctgcatgcc gccattttcg cgaacttctt tcaaaaaacc gccgaactgg
     1081 ccgttatgtc ccatcatcag gtgctgggta aagcccagtt tcgaaaggtt atcaaacaga
     1141 tagcagtcgt tatttgccgg ttgatacaga ttagtgtgcg aagtctgccc gcagctggcg
     1201 cgcagtaaac ggatcgccgc cgggccactg taggaggtgg cggagttaaa gttcttgaac
     1261 tcaatatcga aatgcgacca cagtggatgc gacatcaacc cggcggcttc tatatccgac
     1321 caggaaagcg aacagatgtt aatcaccagt agttcaaatg gctgagcatc agcgggcagc
     1381 gaagacggga aggtcgattt acgtttcgcc tccgcgttat agaaattatt cagccaggcg
     1441 ttaaggttcg ccgttgttgg cggtgcagtt tgtgcgggca tatcacccac taccgatgcg
     1501 ccacccgtcg ccgcaacggt tgccgctgcg ttaccacccg tcgttgttac agtggtcgtc
     1561 ggttgtccgg ctggccacaa ggagaaactt ggtcccgcca gggtaagtac gttcagccat
     1621 agcagtatgg caaccacaaa aacggtaatg cgaatccatt gtgacaggaa taaccaggcc
     1681 actaataaaa caaaaatggc cccaatcatc tgccagttaa taaagcgtgt gacaaggtcg
     1741 attaaataat cggtactgaa ccccgccacc tgcgaaccct ggctcattat gctttccggg
     1801 ccaggcaacc aggtgtcatg ccagaacaaa gcaaagccga tcggcagggc aatccagtgg
     1861 cgcaagcgat gcaggctgta gcgcggaatg ggcatcagca gaaacgtggc aaacaccaaa
     1921 ttgaggagcg gatggaagtt aagatatccc gcccacaaca ggccgaactt aaccagaaaa
     1981 tagaagttcc agccggaaag gccgcgccag tattgccaga gggaagaagg catggcggta
     2041 ttttgcgtaa attgagtcat tttttggttg ccctggcttt ttccgtgcgg cgtaacgtcc
     2101 cggccggttt aacataacga ggtttagcaa agaataaacg taaggtgtcg cgaatgcgtc
     2161 gcaaagagtg ccgcgccaga tagcccagcg ggaaaaatat cagtgcgcaa acgacaataa
     2221 tttcaatgat atcgctgatg gtcatcatga tgagcgctcc acagcatcat ctaacagtcg
     2281 catgggttct ggtattcgtc gccagtggcg accatcgtgc tcggcattga tgaccggttt
     2341 agaactttgc gttaaaggca gcggcatgcc ccattgttct ggggcaagca agcgcatctg
     2401 caccagctcg gcactgattt gatcatcttc aaaccagacc atacggtttg agaaaatgtc
     2461 gccagtaggc aatgggaaaa tatgattcaa cgcggtatcc agatcgttaa tccgacagaa
     2521 tgagagaaac agcaccagcc gattaccgcc aatggtcatg atatcgccgg tacggttagg
     2581 gcgacacagc gtcagggctt gttcaacgcg gatacccggt accggacgta gggcaaccag
     2641 aacgccttta ccgtgggcag gtaatagagg gttattcatc atgttgttga cggcattaca
     2701 gaacacatcc cacttctgga aaccacgcag tttgagcggc tgggtcattg acagcaaggt
     2761 agtgatatct tccggcacat agcgactaaa cttctgccct tgcacgcttt cgatcatcgt
     2821 cagacaacgg gagagtggcg cattccacgg aataaccata tttgcaccgc aggccaataa
     2881 caaacgttca tcggtggcgc gcaggctagc ggtattttcc cgcacgagga ttttcatcgc
     2941 actaccgcgc tggcgacgca gggtatgaat gctgcgggcc agtggctcga tttgcgcatt
     3001 ttgctgtaaa gaaaagacca ccgtcgccgc ctgagcggta cgggcttcat tgaacaggac
     3061 ttcgttattg ttgaacagtt gccagtgttc cgatagcggc ggcgcacctt ccagtacagc
     3121 aacattactc agaatgcgtt tttcgtcgct gcgtggttgg atctccgcct cttcgctttg
     3181 aactaatgtc cagataccat tttgttgctg aacgctaagc tgctgacggg cgctgacccc
     3241 tttttcgttg caccagaagg caatatccag caaatgttgg tcaccctgaa aacgcaaact
     3301 ggcaagacca aaaagtgaac ggtattcctc aagcaacaat gaaaattgtt tatcgttatt
     3361 atttccggga ttaattacca aaagcgaaca atggtttaac ctgctccatt tattcatttt
     3421 atccaaccat gagcgaagcc gctcggcagg aatgttttgc catgcgttat ttgcgcaaac
     3481 aagaataaaa aggtaattat ggggatcaat agaacactgc aaatcacggg ccaaatagta
     3541 tagacctttt tcatgattta gcattgaaaa taattttatt ttttccggcc cttgagaatc
     3601 atctaattga aagattttcg ccggatcgct gtccatgcta atgaccgcga cgtgtgcggt
     3661 ttcagcctgg gatgcaattg tttgattcgc cagactgata gcatcttcat ggcgatcgac
     3721 gttaaaccac cagacgccgc ctgctggcat atgtcgcagc tcatcccata atgatgagat
     3781 accgatagag aatacagggt ccacaatgtc cctcatgtca ccattttgtc tgattatcag
     3841 tttactagcg aa
//
LOCUS       DAESHI010000225         3834 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.228, whole genome shotgun sequence.
ACCESSION   DAESHI010000225 DAESHI010000000
VERSION     DAESHI010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.228"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..266)
                     /locus_tag="KW011_004297"
     CDS             complement(<1..266)
                     /locus_tag="KW011_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBH7662461.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISL"
     gene            353..760
                     /gene="nudG"
                     /locus_tag="KW011_004298"
     CDS             353..760
                     /gene="nudG"
                     /locus_tag="KW011_004298"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HBH7662462.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(726..998)
                     /locus_tag="KW011_004299"
                     /pseudo
     CDS             complement(726..998)
                     /locus_tag="KW011_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
     gene            1234..2577
                     /gene="gdhA"
                     /locus_tag="KW011_004300"
     CDS             1234..2577
                     /gene="gdhA"
                     /locus_tag="KW011_004300"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBH7662463.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(2694..3734)
                     /locus_tag="KW011_004301"
     CDS             complement(2694..3734)
                     /locus_tag="KW011_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBH7662464.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMTSISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
BASE COUNT         1000 a          932 c          916 g          986 t
ORIGIN      
        1 tcaagagaaa tatcaagaaa gccgcccgcg tcggtaagct ctctgcgccg cgccagcgca
       61 ccgtccagac catcaagcag cctgttcaac aaaatgacga ctagcgccgc cagataccag
      121 cccagcgcca gaaacggcag agccagcacg ccgatggcaa atcccactaa tgttaaacca
      181 tctggcgtaa tgcccggttt atcaagaacc cgcacgcact gatgcaacaa cggtttaatc
      241 cggggatgaa gatggcggtc tagcacaggc actccttaaa tataaagcct ttctgattga
      301 gcaacagtgc ggatattatg gcatttttcg cttatctgcc cgtgtgtaat ttatgaaaat
      361 gattgaagtt gttgccgcca tcattgaacg tgatggcaaa attttactcg cgcaacgccc
      421 cgcccagagc gatcaggcgg gattatggga gtttgccgat ggtaaagtcg agctggatga
      481 aagccagcag caggcactgg tgcgtgagtt aaacgaagaa ttgggcatcg aagcaactgt
      541 gggtgagtat gttgccagcc atcagcgaga agtttcgggc cggattatcc atcttcatgc
      601 ctggcacgta cccgacttcc acgggacgtt acaggcacat gaacatcagg cgctggtctg
      661 gtgctcacct gaagaggcgc tgcaatatcc gctggcccct gctgacattc cactattaga
      721 ggcgtttatg gctttacgcg ccgccagagc agcggattag tgctaagggt tttgtcatcc
      781 cgctggcatt acagcagtat tccttcggct ttaattaccg ccccttcaga ataattttga
      841 tcctgataaa cgcagcactg agtacagggc tgcgctgact gcccgcctga actgaacact
      901 tctggcggta cgtttacctc cacgtccgga cgataatgcg ggttagccag tgcaattaat
      961 ggaaatgcta atactacggc gaacaatgct cgactcacag ggaactcctt aacgttattg
     1021 tctctgcaac tgataacggt agccgggtgg caaaacttta gcgtctgagt tatcgcattt
     1081 ggttatgaga ttactctcgt tattaatttg ctttcccggg tcattttttt cttgcttacc
     1141 gtcacattct tgatggtata gtcgaaaact gcaaaagcac atgacataaa caacataagc
     1201 acaatcgtat taatatataa gggttttata tctatggatc agacatattc tctggagtca
     1261 ttcctcaacc atgtccaaaa gcgcgacccg aatcaaaccg agttcgcgca agccgttcgt
     1321 gaagtaatga ccacactctg gccttttctt gaacaaaatc caaaatatcg ccagatgtca
     1381 ttactggagc gtctggttga accggagcgc gtgatccagt ttcgcgtggt atgggttgat
     1441 gatcgcaacc agatacaggt caaccgtgca tggcgtgtgc agttcagctc tgccatcggc
     1501 ccgtacaaag gcggtatgcg cttccatccg tcagttaacc tttccattct caaattcctc
     1561 ggctttgaac aaaccttcaa aaatgccctg actaccctgc cgatgggcgg tggtaaaggc
     1621 ggcagcgatt tcgatccgaa aggaaaaagc gaaggtgaag tgatgcgttt ttgccaggcg
     1681 ctgatgaccg agctttatcg tcatctgggc gcggataccg acgttccggc aggtgatatc
     1741 ggcgttggtg gtcgtgaagt cggctttatg gcggggatga tgaaaaagct ctccaacaat
     1801 accgcctgcg tcttcaccgg taagggcctt tcatttggcg gtagtcttat tcgcccggaa
     1861 gctaccggct acggtctggt ttatttcaca gaagcaatgt taaaacgcca cggtatgggt
     1921 tttgaaggga tgcgcgtttc cgtttctggc tccggcaacg tcgcccagta cgctatcgaa
     1981 aaagcgatgg aatttggtgc tcgtgtgatc actgcgtcag actccagcgg cactgtagtt
     2041 gatgaaagcg gattcacgaa agagaaactg gcacgtctta tcgaaatcaa agccagccgc
     2101 gatggtcgag tggcagatta cgccaaagaa tttggtctgg tctatctcga aggccaacag
     2161 ccgtggtctg taccggttga tatcgccctg ccttgcgcca cccagaacga actggatgtt
     2221 gacgccgcgc atcagcttat cgccaatggc gttaaagccg tcgctgaagg ggcaaatatg
     2281 ccgaccacca tcgaagcgac tgaactgttc cagcaggcag gcgtactgtt tgcaccgggt
     2341 aaagcggcta atgctggcgg tgtagcaacg tcgggcctgg aaatggcaca aaacgctgcg
     2401 cgcctgggct ggaaagccga gaaagttgac gcacgtttgc atcacatcat gctggatatc
     2461 caccatgcct gtgttgagca tggtggtgaa ggtgagcaaa ccaactacgt gcagggcgcg
     2521 aacattgccg gttttgtgaa ggttgccgat gcgatgctgg cgcagggtgt gatttaagtt
     2581 gtaaatgcct gatggcgcta cgcttatcag gcctacaaat gggcacaatt cattgcagtt
     2641 acgctctaat gtaggccggg caagcgcagc gcccccggca aaatttcagg cgtttatgag
     2701 tatttaacgg atgatgctcc ccagggaaca tttcttatgg gccaacggca tttcttactg
     2761 tagtgctccc aaaattgctt gtcgtaacga taacacgctt caagttcagc atccgttaac
     2821 tttctgcgat agcagcagat atgccagtaa agaaacccca ttagactatt tttttgataa
     2881 tcttcttcgc tttcgaacaa ctcgtgcgcc tttcgagaag caagcattat ataatgccag
     2941 gccagttctt cttcaattgt cccgttttga aaagctgtgc ttgatatcga ggtcatccat
     3001 gataattccg ccgcccatat tagcttcgcc gaggatttac cggagcgatt attagcgcaa
     3061 tcagagatat agtctgaggg aaaaacagca aatttattca acaaggcgat aacctgctct
     3121 ggggcttcct ccatgtttgc tttaaaggta ttggctccat ggtcgccaga aagaaaatgc
     3181 tccattaagg cacaataact ttcgctatct tcgatacccc attgattctc taaagactcg
     3241 cgtcttttac tgatgatatc gatcgagtca aaaggaagca catgatattg gaaggtatct
     3301 ttaccaggtt caggctttcg cggccagaac tctagcgttt cagaccattg cttatgatag
     3361 aatcgataag gtgcgatcaa ttgtagcgcc tgtaacttct cgatactgag cggctcaata
     3421 cctttagcct gataataatg cagttgttct ttttttgctt taaaaccggc ccgaacaata
     3481 agccccatca taattaatag ataaagaaaa gagcatcccg cggtaatcag gcctctttca
     3541 ttcaaaccgt tggatgttat cgctgcgaac acaaacatta cagcgacaac acatgttaaa
     3601 taaaaccccc acttacaaag cagcatggcc ttattttctt taatcatccg ttcaaaatta
     3661 ctattaaata tttcccagcc attaaaagaa tacttctcgc tcccaggatg gttttgtaat
     3721 aaaacttttt tcatcacatt ccctgttatt acatacttta gaggcgataa ttttatccat
     3781 gcaaaaaaaa tatccaacga attttcttga tccgctggat atttcagaat atga
//
LOCUS       DAESHI010000226         3798 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.229, whole genome shotgun sequence.
ACCESSION   DAESHI010000226 DAESHI010000000
VERSION     DAESHI010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.229"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(99..908)
                     /gene="proC"
                     /locus_tag="KW011_004302"
     CDS             complement(99..908)
                     /gene="proC"
                     /locus_tag="KW011_004302"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBH7662465.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            1028..1486
                     /locus_tag="KW011_004303"
     CDS             1028..1486
                     /locus_tag="KW011_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBH7662466.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1669..2193
                     /gene="aroL"
                     /locus_tag="KW011_004304"
     CDS             1669..2193
                     /gene="aroL"
                     /locus_tag="KW011_004304"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HBH7662467.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            2243..2434
                     /gene="yaiA"
                     /locus_tag="KW011_004305"
     CDS             2243..2434
                     /gene="yaiA"
                     /locus_tag="KW011_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HBH7662468.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            2692..3369
                     /locus_tag="KW011_004306"
     CDS             2692..3369
                     /locus_tag="KW011_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HBH7662469.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            3441..3725
                     /gene="ppnP"
                     /locus_tag="KW011_004307"
     CDS             3441..3725
                     /gene="ppnP"
                     /locus_tag="KW011_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBH7662470.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          952 a          903 c          989 g          954 t
ORIGIN      
        1 tcgtgagtgg ttgaaatcgg cagatttggc gctttacaaa gcaaagaaag ccggacgtaa
       61 ccgcaccgaa gtggcggcct gacgtccggc gaaagtcatc aggatttgct gagtttttct
      121 gatttttcca tacacttcgt catcgcttcg atcactgcgg cacggaagcc tttctcttcc
      181 agtacgcgta ccgcttcaat ggtggtgcct cccggtgagc agaccatatc tttcagtgcc
      241 cccggatgtt ctcccgtttc cagcaccatt tttgcggaac ccattaccgc ctgagcggca
      301 aatttatacg cctgggcgcg tggcatcccg cccagcacgg cggcgtcggc catcgcttcg
      361 ataaacataa atacgtaggc tggcgaagaa ccgctcacac cgaccaccgg gtggatcatc
      421 ggctcagcaa ttacttccgc ttcgccaaag cagcggaaaa tattcagcac atcagcggta
      481 tcttcggggg ttaccagcgc gtttggcgtt acggaggtca tcccggcatt aaccagcgcg
      541 ggagtgttcg gcatggcgcg gataattttc cggtcatggc ccagcgcgcg ggcaagctgg
      601 tcgagcgtga cacctgcagc aatagaaacg accagagagt ctttattcag gctggaggtg
      661 atttcgctaa gcactttaat catgatgcca ggtttaacgg cggcaaaaat gatgtcggcg
      721 atttgcgcca cttcttgcgc cgattctgcg gcgttgatgc cgaactggtc atgcagggcg
      781 gcgactttat ccggggaggg ggtgtatacc cagatttgcc ctggaagcac ctgaccgctg
      841 gcaatcagac cgccgagaat ggcttttccc atattgccgc agccaataaa accgattttc
      901 ttttccattg cctcactcct gccgtgaaat tcattgtttt gataatcgct ggcagaagca
      961 taaacagaac tatgccggaa ggcaaaagcg cgacacaata gaggattacc caacaaagga
     1021 tgactttatg acaatttggg tggatgccga cgcgtgtccc aatgtaatta aagagatttt
     1081 gtatcgcgcg gcggaacgta tgcagatgcc gctggtactg gtagcaaacc agagtttacg
     1141 cgtgccgcca tcgcgattta ttcgtacgct gcgcgtcgcg gcaggtttcg acgttgccga
     1201 taacgaaatt gtccggcagt gtgaagcggg cgatttggtg atcaccgcag atataccttt
     1261 ggctgctgaa gccatcgaga aaggcgcagc ggcgcttaat ccgcgcggcg aacgttacac
     1321 gccagcgacc attcgtgagc gcctgacgat gcgcgatttt atggatacct tacgtgccag
     1381 tgggatccag accggcggac cagatagcct ttcacaacgt gaccgccaag cctttgccgc
     1441 ggagctggag aagtggtggc tggaagtgca acgtagtcgt ggctaaatgt aatttattat
     1501 ttacacttca ttcttgaata tttattggta tagtaaggga tgtattgaga ttttcacttt
     1561 aagtggaatt ttttctttac aatcgaaatt gtactagttt gatggtatga tcgctattct
     1621 catgacaccg gctttcgccg cattgcgacc tattggggaa aacccacgat gacacaacct
     1681 ctttttctga tcgggcctcg gggctgtggt aaaacaacgg tcggaatggc ccttgccgat
     1741 tcgcttaacc gtcggtttgt cgataccgat cagtggttgc aatcacagct caatatgacg
     1801 gtcgcggaga tcgtcgaaag ggaagagtgg gcgggatttc gcgccagaga aacggcggcg
     1861 ctggaagcgg taactgcgcc atccaccgtt atcgctacag gcggcggcat tattctgacg
     1921 gaatttaatc gtcacttcat gcaaaataac gggatcgtgg tttatttgtg tgcgccagta
     1981 tcagtcctgg ttaaccgact gcaagctgca ccggaagaag atttacggcc aaccttaacg
     2041 ggaaaaccgc tgagcgaaga agttcaggaa gtgctggaag aacgcgatgc gctatatcgc
     2101 gaagttgcgc atattatcat cgacgcaaca aacgaaccca gccaggtgat ttctgaaatt
     2161 cgtagcgccc tggcacagac gatcaattgt tgattttaga gcgcctatac ttaacgttca
     2221 tcccgtgaaa taaggaagaa cgatgccaac gaaaccgcct tatcctcgtg aagcatatat
     2281 agtgacgatt gaaaaaggaa agccaggaca gacggtaacc tggtaccaac tcagagccga
     2341 tcatcctaaa ccagactcgt tgatcagtga acatccgacg gctcaggaag cgatggatgc
     2401 gaaaaaacgc tatgaggacc ctgacaaaga gtgaccgcat cagactgctc ggaagggatt
     2461 ctgagtgcca ctacaaggga tctgcgtcac atttttcata attcatgttt ttctaataat
     2521 tagaatatta aacaataaca atccattact ggaatcattt ggaatcttta cattatgccg
     2581 tgcacgtctg ctgctacgct ttttgtcatt tgtagcacaa gtaagtgtca gcagtggtgc
     2641 ttcacacttg cccggtaatt aacgacgaaa gaaaagtaag gtggatgaac aatgagtgcg
     2701 tcgttggcga tcctgaccat cggcattgta cctatgcagg aggttttgcc gctcctgacg
     2761 gaatacattg acgaagataa tatttcccat catagcctgc tggggaagtt aagtcgtgaa
     2821 gaagtgatgg cggagtacgc gccagaagca ggcgaagaca ccattctcac attattaaat
     2881 gacaaccagc tggcccatgt ttcgcgtcgt aaagtggagc gagacctgca aggtgtggtt
     2941 gaagtgctcg ataatcaggg ttatgacgtc attttattaa tgagtacagc aaacattagt
     3001 agtatgactg cgcgtaatac gatctttctt gagccgtcgc gaatattgcc tccactggtt
     3061 tcctctattg ttgaagatca tcaggtgggg gttatcgttc cggttgagga gatgctgacc
     3121 gttcaggcgc aaaaatggca aattttgcag aaatcgccgg tattttcatt gggtaacccc
     3181 attcatgatt cagaacaaaa aatcattgat gccgggaaag aattactggc aaaaggggct
     3241 gatgtcatca tgctggattg tttaggattt caccaacgtc atcgcgattt actgcaaaaa
     3301 cagctcgatg ttcctgtctt gctgtctaac gtattgattg cacggctggc tgcggaatta
     3361 ctggtgtaat tttgcgtgac agccagcgcc tctggcccct atagtgaagt agatgttcaa
     3421 ctaccagaca gggccagttt atgcttcaaa gtaatgagta cttttccggc aaagtgaaat
     3481 caatcggctt ttccagcagc agcactggtc gcgccagcgt gggtgttatg gttgaaggcg
     3541 aatacacctt cagcaccgct gagccggaag agatgacggt aatcagtggc gcgctgaatg
     3601 tgttactgcc tgacgcgacc gactggcagg tgtatgaagc cggttcggtg tttaatgttc
     3661 ccggtcacag tgagtttcat ctgcaagttg ccgaacccac ctcttatctg tgccgctatc
     3721 tgtaattcct cgccttcccc ttgaacggga gggcattttt ctgaaatatc ctctctttaa
     3781 cccataataa tatttcct
//
LOCUS       DAESHI010000227         3734 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.230, whole genome shotgun sequence.
ACCESSION   DAESHI010000227 DAESHI010000000
VERSION     DAESHI010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.230"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..182)
                     /locus_tag="KW011_004308"
     CDS             complement(<1..182)
                     /locus_tag="KW011_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HBH7662471.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSI"
     gene            426..683
                     /locus_tag="KW011_004309"
     CDS             426..683
                     /locus_tag="KW011_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HBH7662472.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(733..1683)
                     /gene="uspE"
                     /locus_tag="KW011_004310"
     CDS             complement(733..1683)
                     /gene="uspE"
                     /locus_tag="KW011_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HBH7662473.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1835..2587)
                     /gene="fnr"
                     /locus_tag="KW011_004311"
     CDS             complement(1835..2587)
                     /gene="fnr"
                     /locus_tag="KW011_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HBH7662474.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2782..3297)
                     /gene="ogt"
                     /locus_tag="KW011_004312"
     CDS             complement(2782..3297)
                     /gene="ogt"
                     /locus_tag="KW011_004312"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBH7662475.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3308..>3734)
                     /locus_tag="KW011_004313"
     CDS             complement(3308..>3734)
                     /locus_tag="KW011_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBH7662476.1"
                     /translation="FIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWSI
                     LAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLGT
                     YYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT         1003 a          886 c          893 g          952 t
ORIGIN      
        1 ataatgctac caacagcact gataattatg gtgtagatta aaaattgcac cgcatggaat
       61 aaaaaacctt ttcttttacg atttccgcgg cgaaaccaaa agctcatcag aatcaatgct
      121 gcgcagctac cgaaaataat gaccagatta agcgcattat ttgtaaacag ttcagcgatc
      181 attgttttat caggctcctc cagataattg tcgtcatgcc ggaaacccct ggcggggcta
      241 ttttaccgcg acaattcatt cagatcatca atagtcaggg aaggaagtag caacattagc
      301 taactaatca atgcgttgat tgtaaatcca gctaagaggt gaggttttca gagcagacaa
      361 cggtgaaatg tcatggtatt gttacgttta ggtaacaaga aatttgtctg cacaaggatt
      421 acatcatgat tatggcgaaa ctgaagtcag cgaaagggaa aaaatttctc tttggtttgt
      481 tggcggtttt cattattgcg gcgtcggttg tgactcgcgc gactatcggc ggcgttatag
      541 aacagtacaa tattccgctg tctgagtgga cgacatcaat gtatgtgatt cagtcatcga
      601 tgatttttgt ttatagcctg gtctttactg tgttgctggc aatcccgttg ggaatttatt
      661 tccttggcgg cgaagagcag taagtaaaaa ataggcccga taactcgggc cttgtcagtt
      721 attgaagagt cgttaatcgt cttcttcgtc atccagttca acgggtgtct gatactggtc
      781 aggtttaatg accaacagat cgcagcgaag atgatcaatc acctgttccg ccgtgttgcc
      841 gaggaatgct gctgaaatac cggtgcgtcc taccgtgccc agaaccacaa tccccgcctg
      901 taagtgctcc gccaaatcag gaataacctc ttccggcaga cctttttcta cgtgcgtcat
      961 gttttcatta atgccgaatt tctgccgcag agctttcatt gccagcaaat gttgcccacg
     1021 aatggcatcg ttataaacgc tcggatcaaa ttccggcagt tcaatcgcga tattaattgg
     1081 cgttactggg taagcgccaa ccagatgaac ttcggtatgg ttgacttgtt ctgccagttc
     1141 gatcgtctct ttgaccagtt tttcattgag cgcattatga tacggctctt cactggcgag
     1201 attcaccgcc accagcgcct tacctccttc cggccacggc tggtctttca ccatccacac
     1261 cgggcttggg catttgcgta acagatgcca gtccgttggc gtaaaaatca ccgcttccag
     1321 acggtcatgt tggtgcgcca tttttagcac caaatcgtgc ccgccgctga tcacttcctg
     1381 aatgatggct tcgaagggac ggttatgcca gaccacttta atttcaatgg gaacgccagc
     1441 attgagataa tattttgcct gctcgtggat ccaggctgta cgctggctga tgacgccctg
     1501 acgcatagcg gtacgttcgt ccggggagag cagggtggtc atttcgtatg agaagtcata
     1561 gatcggcaaa aaggctttaa ttttgccacc aatccgttga tgtaaataaa cagctcgccg
     1621 caatgctggt tggtcgtcct ggttaggatc gataacaacg agcatgttct gatacatagc
     1681 catacagggt ctccttacaa caactgtcaa cgcagtttgt aattaaaaga ttaacccata
     1741 tctggtgaat gaaacagtga tgaaccttct gccagatcaa taaatcagaa aaatttaatg
     1801 atatgacaga aggatagtga gttatgcgga aaaatcaggc aacattacgc gtatgaccag
     1861 caagctgggc cagcgcatcg ttattttcga tggtgatgta tttacctttg actgccagca
     1921 taccgctttt ctggaagcga cctagcagac ggctgatggt ttctaccgtc aggcccagat
     1981 agttaccgat atcgccacga gtcatcgtca ggcggaattc acgaggggag aagccgcgtt
     2041 gggcaaaacg acgggacagg ttgtagatga atgcagccag acgttcctcg gcatttttct
     2101 tcgacaacag caagatcatg tcctgatcgc ctttgatttc accgctcatc agacgcatca
     2161 tctgctgacg cagattcggc attttaccgg acaaatcgtc cagcgtttcg aacgggattt
     2221 cacataccat cgaggtttcc agcgcctgag cgaagctcgg gtgatggccg ctgccgatgg
     2281 cgtcaaatcc caccaggtcg ccagctaaat ggaaaccagt gatttgctcg tcgccttgct
     2341 cagtgatggt ataactttta atcgtaccgg agcggatggc ataaagcgat ttaagttcat
     2401 caccagcctt aaacagcgtc tggcctttct gaataggctt cttccgctca atgatattat
     2461 caagctgatc aagctcatgt tcgttgagtg tgaacgggat gcaaagctgg ctgatgctgc
     2521 aatcctggca atggatagca caaccgccag actgaatgcg ccgtataatt cgcttttccg
     2581 ggatcatagg tctgctcaag ccgtaattga tatttgtcaa ttttaacatc tttttaggga
     2641 gcaagtaagt ctaagcaaac cttaacagca gagaattccg atattagatg taaatatatg
     2701 tctatctatt tgaaaaccct taagttgtta agggtaactt tacataaaag tgtgaacaag
     2761 ctggcacaaa ttgtttaatg tttacagcaa aagataacct tcgtggcgca ataaccactc
     2821 ttttcgctga actccgcctg catatccggt catggtgccg tttcggccaa taacccgatg
     2881 gcaaggtacg acgatgctga tgggattcga tccgtttgcc gcaccaacgg cacgcgccgc
     2941 gccagggcgg cccaattgtt cagccagttg gccgtaatgc attacctgtc cgcaggggat
     3001 agtgcgtaga gttttccaga cttcgcgctg aaatggcgtc ccccctgtgg cagtgggaag
     3061 cgtatcaata atgctaagat taccggcaaa ataatcacga agcttgtcgc ttaaaccgcc
     3121 tgggttggtg gcagaaatgc gctcatagcc ttctttgcga taatggatgt ccagcagctg
     3181 caccatgcgt tcgctgtact cttcccattc aaccgcccgc aggcgaaatt gctcatcgca
     3241 aatcacccac agtggaccca gtggcgtggc aattttttct tcaagtaatc tcagcatccg
     3301 ttctctctta agacaaacgt gggtaaatcc ccggacctat cggcagacca acaagatacc
     3361 acgccagcaa catcagcagc cataccacca aaaagataag cggatagggc aagactaacg
     3421 aatagtaagt acccagtttc gcgtctggtt tgtagcgttg caggaatcca agaaacagtg
     3481 gaacaaaagg agataccggc gctaaaggca atacggatga gtcggcaata cgaaagagga
     3541 tttgcgcaaa tgccgggtga aagccaagta gcataaacat tggtacgaaa atgggggcca
     3601 gaatcgacca gattgcggaa ccgctggcaa taaacatgca taagaaagag gaaagcaacg
     3661 ccagaccgac aaacgccggg atgccgctaa gccctgaact ttccagtata tcggtcagcc
     3721 ccacggcgat gaat
//
LOCUS       DAESHI010000228         3674 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.231, whole genome shotgun sequence.
ACCESSION   DAESHI010000228 DAESHI010000000
VERSION     DAESHI010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.231"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..95
                     /locus_tag="KW011_004314"
     CDS             <1..95
                     /locus_tag="KW011_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBH7662477.1"
                     /translation="RLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            244..1491
                     /locus_tag="KW011_004315"
     CDS             244..1491
                     /locus_tag="KW011_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HBH7662478.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(2020..2220)
                     /gene="torI"
                     /locus_tag="KW011_004316"
     CDS             complement(2020..2220)
                     /gene="torI"
                     /locus_tag="KW011_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HBH7662479.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2343..2687)
                     /locus_tag="KW011_004317"
     CDS             complement(2343..2687)
                     /locus_tag="KW011_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662480.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2929..3531
                     /locus_tag="KW011_004318"
     CDS             2929..3531
                     /locus_tag="KW011_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662481.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
BASE COUNT          920 a          740 c          844 g         1170 t
ORIGIN      
        1 cacggctggg acggaagtcg ctgtcgttct caaaatcggt ggagctgcat gacaaagtca
       61 tcgggcatta tctgaacata aaacactatc aataagttgg agtcattacc cgcttgcagt
      121 aacggcgaaa ggggcaatga cagcgctgcc atgtcgacaa gaactggaat agtgagaagt
      181 aaacggcgaa gtcgctctta tctctaaata ggacgtgaat tttttaacga caggtaggta
      241 attatggcac tgaatattcc attcagaaat gcgtactatc gttttgcatc cagttactca
      301 tttctctttt ttatttcctg gtcgctgtgg tggtcgttat acgctatttg gctgaaagga
      361 catctaggat tgacagggac ggaattaggt acactttatt cggtcaacca gtttaccagc
      421 attctattta tgatgttcta cggcatcgtt caggataaac tcggtctgaa gaaaccgctc
      481 atctggtgta tgagtttcat cctggtcttg accggaccgt ttatgattta cgtttatgaa
      541 ccgttactgc aaagcaattt ttctgtaggt ctaattctgg gagcgctctt ttttggcctg
      601 gggtatctgg cgggatgcgg tttgcttgac agcttcaccg aaaaaatggc gcgaaatttt
      661 catttcgaat atggaacagc gcgcgcctgg ggatcttttg gctatgctat tggcgcgttc
      721 tttgccggca tattttttag tatcagtccc catatcaact tctggttggt ctcgctattt
      781 ggcgctgtat ttatgatgat caacatgtgt tttaaagata aggatcacca gtgcgtagcg
      841 gcggatgcgg gaggggtaaa aaaagaggat tttatcgcag ttttcaagga tcgaaacttc
      901 tgggttttcg tcatatttat tgtggggacg tggtctttct ataacatttt tgatcaacaa
      961 ctttttcctg tcttttatgc aggtttattc gaatcacacg atgtaggaac gcgcctgtat
     1021 ggttatctca actcattcca ggtggtactc gaagcgctat gcatggcgat tattcctttc
     1081 tttgtgaatc gggtagggcc aaaaaatgca ttacttatcg gtgttgtgat tatggcgttg
     1141 cgtatccttt cctgcgcgct gttcgttaac ccctggatta tttcattagt gaagctgtta
     1201 catgccattg aggttccact ttgtgtcata tccgtcttca aatacagcgt ggcaaatttt
     1261 gataagcgcc tgtcgtcgac gatctttctg attggttttc aaattgccag ttcgcttggg
     1321 attgtgctgc tttcaacgcc gactgggata ctctttgacc acgcaggcta ccagacagtt
     1381 ttcttcgcaa tttcgggtat tgtctgccta atgttgctat ttggcatttt cttcctgagt
     1441 aaaaaacgcg agcaaatagt tatggaaacg cctgtacctt cagcaatata gacgtaaact
     1501 tttccggttg ttgtcgatat ctccctatcc ctcaaccgga aaataataat actaaagtgc
     1561 ttagcccggc taataatcac ctaatccaaa cgcctcattc atgttctggt acagtcgctc
     1621 aaatgtactt cagatgcgcg gttcgctgat ttccaggaca ttgtcgtcat tcagcgacct
     1681 gtcccgtgta tcacggtcct gcgaattcat caaggaatgc attgcggagt gaagtatcga
     1741 gtcacgccat atttcgctat caggattctg tgtgatggtt acatcgcccg gcccagggct
     1801 gtttagtcat cagcgctttc tgacagtgct gagatttcaa cctgttgcag taaaaatgag
     1861 tagatataag gcaagtgtgc tgccaaaccc atcttttacg gggtgaaggt agatttcgtt
     1921 tgaagggtat ctggtgtccc ctgcagacat ctacttgagg cggcagggga ttgattggaa
     1981 tgctgttttt tagatttgaa aaatatttta cccgctattt tacccattgg cgcggcttaa
     2041 gagcttattt ttaaattcac aatggtcacg atataaccat cttgctcgtc cgtggataac
     2101 tttggcttta ggcaggtcgc cggacttaat ccggtcatag atgaaggtct taccgaagcc
     2161 agtatcagcc atgatgaatt tcaaatcaac cagtgaatca ggttgtagtt cgtgttgcat
     2221 gagtgctatc tccgaatatg gaatcgaacc tgcaaatcag gcaataaaaa gccgcattga
     2281 tgcagcaatg gtaggtctgg atatcttgag aaatgaacag gcctcatcga gtgtgaggcg
     2341 ggttagtcct tgcgtagctc gctgattctt ctgtaagtct ctggtgcttt gttcccgtac
     2401 gtcttcattt cagacttcaa cagagcaacg agtgaatccc attcgttgag gattcctttg
     2461 aatgccggaa cgcgctttgc aaccttgtcg aatgaatctc tgatttctgg aatctgctca
     2521 acaagtgcaa cgcatcgccg aaagtctgct gcgtcatggg gagcgccgaa gtgatgacca
     2581 tagatattct ttttcaggcc acatgcgatt gaggcaagag ttgcgctact gatgccgaca
     2641 tcgccagttg attgccattt caaaaccttc atagccaaat ctgacatttc ttgtctccat
     2701 aaaacaaaac tcgccgtagc gagctcagat aaaagaaatc cccgtcagtg cgaggatgct
     2761 gttcattgct gctatacact tttttgctct caacgtaagc ggtagcccat tctgttgggt
     2821 tggtcagttg cttttaggaa atgctcttta ccccttaaac gtcggctgaa agagctaaaa
     2881 tccatgcaaa aaatttacgc aattttgtgt attattgtgc agtaagtaat gagctatttt
     2941 ctgtgcaaaa aatggatgat aaatttgtcc ggggcaggaa aaattttatg ggcgctaaac
     3001 atgaaaaaag attcgtatcc ttatttgatt tgcatgacag tttcagggct gatctttatt
     3061 ttccttttct tctggtggcg ggcagatatc tacagggtca cgtttcttaa tcagagtata
     3121 tcccactatt acattctgtt tagcatggga atagcttttc tgttatctct gttttgggtt
     3181 aagaagggga tagtaaaaca aagcggctgg aagagtctgt cagcatacct taaggtttat
     3241 gcagggatat gcatattttc tggatttttt ctgattatac cccttacaac actaacttat
     3301 tttttgcctg gagagacatc gtcttatgtt gcaccgtatc ggtatacttc cggtagttca
     3361 aaaagttgtt ctggagctga ggtggatgac cccgatctac atgagaatat tcgcatttgc
     3421 tatccgtatg gcaattatga gtacgataat attatctatg ttgaaaagaa aattaataca
     3481 ttaggtgcgg tagtgacata tgcacagacc gcgcgtgatg atactgaatg agatagtata
     3541 tagcgggcaa gttttagtta atttatcgag gtaatataat ttacctcgac tcgtttattc
     3601 tggtatgaat atttcgcttt acgatcgatt tttatctgat gatatcatgc ggttttcata
     3661 tactgactta ctgt
//
LOCUS       DAESHI010000229         3627 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.232, whole genome shotgun sequence.
ACCESSION   DAESHI010000229 DAESHI010000000
VERSION     DAESHI010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.232"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(89..652)
                     /locus_tag="KW011_004319"
     CDS             complement(89..652)
                     /locus_tag="KW011_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBH7662482.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(756..974)
                     /locus_tag="KW011_004320"
     CDS             complement(756..974)
                     /locus_tag="KW011_004320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662483.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(1133..1519)
                     /locus_tag="KW011_004321"
     CDS             complement(1133..1519)
                     /locus_tag="KW011_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBH7662484.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(1509..1880)
                     /locus_tag="KW011_004322"
     CDS             complement(1509..1880)
                     /locus_tag="KW011_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBH7662485.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(1880..2137)
                     /locus_tag="KW011_004323"
     CDS             complement(1880..2137)
                     /locus_tag="KW011_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662486.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            complement(2134..3525)
                     /locus_tag="KW011_004324"
     CDS             complement(2134..3525)
                     /locus_tag="KW011_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBH7662487.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTG
                     RNIWMVDQANLTIDEICATTKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQATGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYS
                     NTRTDSSKMERFF"
     gene            complement(3522..>3627)
                     /locus_tag="KW011_004325"
     CDS             complement(3522..>3627)
                     /locus_tag="KW011_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH7662488.1"
                     /translation="FISPRIADRITDGGRNWLSFNWPSYRSCIRGVAA"
BASE COUNT          850 a         1013 c          811 g          953 t
ORIGIN      
        1 catcggcggg tttaagctgt gtggcgtagt aaccactctt aacaggatat tcaacttttt
       61 acgatcgtaa agttttcgga aaaaattttt aaaaccgcat caaccgctcc accagttgct
      121 ctttacgggc aacgatccac ccgtgttgct ccagataaaa tttaaaccgt tccagagtac
      181 ataccatcgc atcggcgggt accttttccg tgaactcaac ctgaccgtgt ttatcgaagt
      241 ggctcagtaa tgcgcatcca tcattttcga tagatgtgtt ttgtgccgct ggtggctgtt
      301 tctggttgaa ataacagtct tccagttttt cgaacacttc ccacgcctga tcggtttcga
      361 gcattttggc gtgacgggct gcgccacgtt ctgtccagag gatgagggag cgaacgttac
      421 gggcaatttt cacagaaact cttaaagagt tttcgttctt caactctcgt aacgcatcac
      481 cttctatcag gaaaaaatgt ttaccttcta taaaccgttc tttgtttcga ttgaaattgt
      541 ttgtcaaacg ctggcgctct gttccgtaca aatcagccag cagctctgtt gtcatgacag
      601 gaagatggtt atgcgtaagt gacgggagtt tttcaataga cgtgcgattc atagacatgt
      661 cctttcgaat attttgattt accccttttg agagggtgac cgggcgctca aaaccgtcga
      721 aagtcggcgg gcgtatttcc attgctggta ttgtattagc cgcacacccg gtcataaacc
      781 aagaattctg gacacaaaaa aaccacatgg ctatcgggtg tggataccgc tttcgaggtg
      841 ttttgagcac cgcggggaaa tgtaaactgc aaacctgaaa ctgtcaacac cgtcaggttc
      901 caatcatcgg gtggtgagac gcacagggtt ggaactaccg ggaaaccgac cggcgagctt
      961 ttcagctccc ccatgcgccc caccataatt cagatgtgcg cgtgcatacg acaataaaaa
     1021 acacgctcgc ggcgtgtgtc tgtcgcggtc tctatccggg gttccaatcc cgacgccaga
     1081 ttttgctggc gcgtgaggaa tatagccccg gacaatgtgt ctggtcaagc acctacataa
     1141 ttcgttctac gtatctgtcc atctccagcc ggatatcaag catcatcaac atgccatcaa
     1201 taaccccttc cgctttctgc aggcgcttgc caatacaggt atccgaacac ccatgtttcc
     1261 gtgccagact cataaaagtc attccaccta cgtaataatc caccagcaaa tcgtgcaaat
     1321 cctgattttt cttgttcaac cgggccatac agccacaaat tatcattgca tcatcatcag
     1381 aacactgagg gcgtgatttc actttcgacg gaattaaccc tttaaaacca gcagcgatcg
     1441 acgcccagga tacatcttca tgagtgtttg cagcccaagc cccccaccgt tccataacct
     1501 gctgaatatc acgcgccatc gttatcacct gtgatttcat aaatcttcac gcccagtcgc
     1561 ccaccaggaa cgagctgacc gcgcacaata ttgatttcat caaactgctc gtcgtctatg
     1621 agtagtccgg catgcgtcag cgcatccagt ggtgctttca ggatattgtc caggtcacga
     1681 cggcgcttat ccggtggctc tgcaataatc tttatcgcca gccttccgga caggtttaat
     1741 ttcagccgct gctggcgaac aattagcgtc acatcacggc gataacgctc accaactttt
     1801 gatacaaaat atgtgctgcc acgacgtcgc cagtaagtat tcaccgtcgg cggataaggt
     1861 aaaataaact catggcgcat cagcgcagca cctcctgcac cattttttca aactttccaa
     1921 ctttggtttc cagctctgcc acacaatcaa cgagctcatc caccgctttt tgtgcgcgat
     1981 gtttcgcctg catcagttcc ctgagcgctg gcaccatatc ccgacgaatg gcatcttttg
     2041 ttacacctgt tttttccagt tgttccgcct gtcgcaacat ttcctgcgcg tgtttacgca
     2101 attgttcaga ggtaaaagtc attgtctggt tgttcaaaag aaacgctcca tcttactgct
     2161 gtcggtacgt gtattgctgt atctgcgcgg ctggggctgc cgcattgggg tggaaagaac
     2221 ctgtgcgctt tcctggtcca caggcagaaa atgtccgtta taaaaacgcc ggtaaatcgt
     2281 ccccagagaa ccgttacgtt gtttcgtgat attaatttct gctatgcctg tagcctgtgt
     2341 atccgggttg tacacttcat ccctgtaaag catcagaatg atgtctgcat ccgcctctat
     2401 ttctccggaa tttttcaggt ctgagttcat gggacgttta ttgggtctgg actccacacc
     2461 gcgggagagc tggctcagcg caatcaacgg aaaaccaccg gattttgcca ggcctttaag
     2521 cccctttgag atttcaccca cggcaaggtc atgacgcccc gtggttcggg tttttatcag
     2581 cccgagataa tcaaccacca ccagcgccgt ttccggatgt ttaatcagat ggtgtttcgt
     2641 tgttgcgcat atctcatcaa tggtcaggtt cgcctggtcc accatccaga tattgcgccc
     2701 ggtcatccgc cccacccctt gtgagaaacg cgcccagtct tcatcttcaa agtgagccac
     2761 agatttcagg cgtgatactg gcatccctcc agccgcagac accatacgtt caccaatctg
     2821 gatgttcgcc atctccatgg tgaacagaag cacaccatgc ccctgctcag tcaccttgtc
     2881 gatgatgtcc agcgcaagtt cggttttccc catcgaagga cgggcggcaa tgaataccag
     2941 gtctccgggc tccataccgc ctgtttttgc gtccagttca tcaataccgg tcatcaacgt
     3001 cctggatttc tccagcccct gattccggca ttcaacacgc tcaaccactt ccggaagcac
     3061 atcatcaata tgtaccggct gaatgacgcc ctttcctgtc gacagtgtga ccatcatgtt
     3121 ctgcgcatcc ttcagggcat cttcagctgc ttcacaggta tgcgcatcac gtaatttctg
     3181 cagcgcctca ttcagtgttt tttctgcatc gcgcagtgcg gcattgcgcc gcaacgctgc
     3241 aacatagtgc tccagtgaag acttcaccca ggttttacgc ccggtatcag taatcaccgg
     3301 ggcaagttcc ggcatctcat tacacaacag cacggggtca atcactcctg aaacacgggc
     3361 ctgtctgcag atgcctgtgt agatatcccg atacgctcgt acagaaaaaa tgtccgccgg
     3421 tagtgtgatc agaatatcca tcacttcatg atctgcccca cgcagaaaga atgcgccaat
     3481 gacagcgcct tccaggtcat cattacgcca gactggtgtt gtcatgcagc cacacctctg
     3541 atacaagaac ggtagctggg ccagttgaac gacaaccagt tgcgcccccc gtctgtgatc
     3601 ctgtcggcaa tgcgggggct gatgaac
//
LOCUS       DAESHI010000230         3586 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.233, whole genome shotgun sequence.
ACCESSION   DAESHI010000230 DAESHI010000000
VERSION     DAESHI010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.233"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..123)
                     /locus_tag="KW011_004326"
     CDS             complement(<1..123)
                     /locus_tag="KW011_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HBH7662489.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPY"
     gene            384..719
                     /gene="iraM"
                     /locus_tag="KW011_004327"
     CDS             384..719
                     /gene="iraM"
                     /locus_tag="KW011_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HBH7662490.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNSIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1000..1131)
                     /locus_tag="KW011_004328"
     CDS             complement(1000..1131)
                     /locus_tag="KW011_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HBH7662491.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(1631..1707)
                     /locus_tag="KW011_004329"
     tRNA            complement(1631..1707)
                     /locus_tag="KW011_004329"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1671..1673),aa:Arg,seq:tct)
     gene            complement(1808..1883)
                     /locus_tag="KW011_004330"
     tRNA            complement(1808..1883)
                     /locus_tag="KW011_004330"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1848..1850),aa:Ile,seq:cat)
     gene            complement(1986..2840)
                     /locus_tag="KW011_004331"
     CDS             complement(1986..2840)
                     /locus_tag="KW011_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBH7662492.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTRLIF
                     PESVVNSS"
     gene            complement(2837..3211)
                     /locus_tag="KW011_004332"
     CDS             complement(2837..3211)
                     /locus_tag="KW011_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBH7662493.1"
                     /translation="MRHEFILPYPPTVNTYWRRRDNTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            complement(3211..3468)
                     /locus_tag="KW011_004333"
     CDS             complement(3211..3468)
                     /locus_tag="KW011_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662494.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
BASE COUNT         1010 a          950 c          684 g          942 t
ORIGIN      
        1 ataaggggtt atggccaccg gactaccaaa cggctgccag ccctctttca gtttatgtgt
       61 cagcttttcc gcaagatctg acggcggcgc cgccctgaca acatcatagt gtttaaatgc
      121 catggttctt tccaccatct gaaaaataat tctttaaaat acctgacatg taatacagaa
      181 aaaacacaaa accatacctt aaataaaaac ctgattatca agcagatatg catggataaa
      241 ctacaagacg agatataaac caccctgcat ttaaataaac aataaacaac atcagaaaaa
      301 taattctgct ctatggttta cattcaaaaa tatcatttat acttttcaga acatcaccag
      361 caaggcataa acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat
      421 catgcggaat atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat
      481 cggatgcttt cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca
      541 ataacaaagt gctacctgta accatttaca acgtagtacc attcaataaa acattctgga
      601 attcaatcaa aaatagccag gaatgcccta caaatacaga taacgtattg aatgaatgct
      661 ttaataaccg ttgcactctg caaatatgtc cttatggact aaaacaacaa agtccataag
      721 gagtttactc acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag
      781 tggcgctatg tgccattgca tggtgccggg tgcctcccgg tgaattcagt accagcacct
      841 gaatccgcga ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac
      901 catgccagtt tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg
      961 tgaagtattt aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt
     1021 tttcagcaac gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccagcac
     1081 tgacattatc cggctgctgc aatgccattc acaaaaacag taagcaatca ctttttaccg
     1141 taacaggtga taatccagat atgtatctac cccagatgag taatccgaag ttcatccata
     1201 ccacaggtcc tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca
     1261 accatgaact catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt
     1321 cccgaaagac gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt
     1381 gctgcattga ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata
     1441 ttcactctac ccacaccata tgaatgataa atatttaatg ctgaaaaaac cattagaccg
     1501 tataacaaac actcaatcaa tacttaacag aacttttatt tttgacaaac ataaaatatt
     1561 ttcaacaata tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga
     1621 agaatgaata tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat
     1681 tgctctgtcc ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc
     1741 gcccccgcac caacaaccct gttatcatgt cgtctgctct tcctgataag ctaatggcgg
     1801 tttgtgatgg tggcccttgc tggatttgaa ccagcgacct ggcgattatg agtcgctcgc
     1861 tctcaccact gagctaaagg gccgggagca gaataataac ggtccgtaat taattccgca
     1921 ataaaaaacc cgctcggcgg cgggttgtag aaactcttct aacgtcaggc ataaaacgcc
     1981 catagttatg acgaatttac cacagattcc ggaaaaatca acctagttac cttttttaac
     2041 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc atagaatttc
     2101 ttaactgttt tttcccatga cgcgcgtgtt atctggtttg tcacctcgca tatagcatta
     2161 aatgcctccg ttgatggtag tctttcatag ccacgaccac cacaacgctg gcagtctctg
     2221 ataacaggca taccacgttt taccgactct tcacgatgaa tggcgacacc acgcccacgg
     2281 caatccttac aggcggtgga aacctcaccc tttccgccac actccggaca ggcaactttt
     2341 accacctccc tgactttttt ccattcttcc cagtaagacg gatacacacc tttcgtacac
     2401 tttgcccata ccggcggctt accatccgga tactggacct tgtttgtaaa aactacgctt
     2461 tcaataaatt tttccccata gcaacaaggg cactgctttt tactcgctgc gctgcgggca
     2521 taatcctcaa aagcgtacga agccataatg cgcatcacta ccggttttat ttctgccgga
     2581 agttttctca acgccgccac acgatcgcac cgactgagtg cataatctgc cagtaattct
     2641 gttgcccgcg ccctgtcatt catactgatg cccattttcc ccaggaacgc agaaaacccc
     2701 atctcagccc gattctgtgt catgccctgc gcggccatca catcagtgat actcagcgca
     2761 tcttttgacg ttgaggccga tgcatcggtc aggcaagggg attttgggga gtagtatttc
     2821 ggtaaatctt ccagtttcat tttttgacct gctcttcatg cattatgggg taaatcttca
     2881 cccccagacg tccaccagat actggctgac cacgaacgat attgatttca tcaaactgct
     2941 catcgtccat taacactccc gcatgtgtca gtgcatccag tggtgctttc aggatattgt
     3001 ccaggtcacg acggcgctta tccggtggct ctgcaataat ctttatcgcc agtcttccgg
     3061 acaggcttaa tttcagtcgc tgctgacgga caataagcgc cacatcccgg cgataacgct
     3121 caccggcttt tgatacaaaa tatgtgttgt cacgacgtcg ccagtaagta ttcaccgtcg
     3181 gcggataagg taaaataaac tcatggcgca tcagcgcagc acctcctgca ccagtttttc
     3241 aaactttccg actctggttt ccagctctgc cacacagtcc accagctcat ctactgcttt
     3301 ctgtgcgcga tgcttcgcct gcatcagttc ccgaagcgcg ggtaccatat ctttacggat
     3361 agcgtctttt gttatgcccg ttttttcgag ttgttcagca tgacgcagca tttcctgcgc
     3421 gtgtttacgc aattgttcag gggtaaaagt cattgtctgg ttgttcaaaa gaaacgctcc
     3481 atcttactgc tgtcagttcg tttattactg tatctgcgcg gattgccggg cttcatggga
     3541 gtggaaagca cccgtgcact ttcctggtcc acaggcagaa aatgtc
//
LOCUS       DAESHI010000231         3546 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.234, whole genome shotgun sequence.
ACCESSION   DAESHI010000231 DAESHI010000000
VERSION     DAESHI010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.234"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..140)
                     /locus_tag="KW011_004334"
     CDS             complement(<1..140)
                     /locus_tag="KW011_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662495.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAV"
     gene            complement(186..527)
                     /locus_tag="KW011_004335"
     CDS             complement(186..527)
                     /locus_tag="KW011_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662496.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(696..2150)
                     /gene="xylB"
                     /locus_tag="KW011_004336"
     CDS             complement(696..2150)
                     /gene="xylB"
                     /locus_tag="KW011_004336"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBH7662497.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVDSQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESVEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(2222..3544)
                     /gene="xylA"
                     /locus_tag="KW011_004337"
     CDS             complement(2222..3544)
                     /gene="xylA"
                     /locus_tag="KW011_004337"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBH7662498.1"
                     /translation="MQAYFDQLDRIRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
BASE COUNT          926 a          981 c          783 g          856 t
ORIGIN      
        1 acggcaaaat aatacccttt ttcatttaac tgcatctctg cattccatag ccctaacaga
       61 taggtaacga taccaccaac gagagctatc catgacacaa tactaaaggc cggtgaatag
      121 gttgatattt tgttgtccat cacagtattc ctttcattcc tgaatgtgta agagctttat
      181 gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa aaaggcgatg
      241 gggtagatca ccatttcata agcattgata ggtgcattta aaacaccgat aaacaagaga
      301 ccgatagtga ttaacgcaac taactggcag atatgggtaa aaaaattatc cagttgcccg
      361 agtttttctg cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa cacgccaaga
      421 aaatagcctt ttccacttag caatgggcat gagatccata gcccaaccag ataaaccagc
      481 gccccgacaa caaataatag ttttgccaca gtttttgagg ttttcataac gatctccata
      541 tctaccagcg atacattacg agtaaccaac gaaagacaaa actgaaaaat gccattaaca
      601 aatgattttc agaataaatt catactaaat attaattaat tagtgagata tatagatgtg
      661 aattatcccc cacccggtca ggcaggggat aacgtttacg ccattaatgg cagaagttgc
      721 tgatagaggc gacggaacgt ttcacgtcgt ggctgataag cggcataacg ctgcgcatct
      781 ggtagatgcg actgttctaa cggtagctgc ggcaacaact caatgagcga tttctctgga
      841 ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg gccccacatc cccccccgta
      901 cggtaatcga gctgctgacc gctgatatcc gccagcatct gacgccagta ctcactacgc
      961 gccccgccac caatcaacgt aacactttgc ggtttaatgc cgcaggcatg cacgacatcc
     1021 atgccatctg ccagcgcata acccacgcct tccagcactg ctcgcgccag ttcattgggg
     1081 ccatgttgat gagtcaaacc aaagaaaacc cccttcgcct ggggattatt gtgtggtgta
     1141 cgctcgccgg aaagataagg cagaaaccaa actggctcga cactttcatc agcctgttga
     1201 gctgcagcga ttaaagctgg gacattgcac aggccggtta atttcgcggc ccaatccaga
     1261 cacgatgctg cactcagcat tacagacatt aaatgccaac gctgcggtag cgcatggcaa
     1321 aagctgtgta cagcgctttc tggcttgctt aagaaccctt cgctgacagc aaaatagacc
     1381 cccgacgtcc ccagcgataa cattgcctga ttagcatcaa ccattcccac accaactgca
     1441 ccagctgcat tgtcgccacc gcctgcgaca actggcaccg tcgccatacc ccacgctttc
     1501 gcaacttcag gtaacaaagc accagtaatt tcgctgcctt cgtataatgc gggcatctgg
     1561 tcacgagata agtcgcaagc ctgcagcatg acgtcactcc agtcacgctt tgcgacatcc
     1621 agccacatgg tgccagctgc gtcagacata tcgctggcaa actcccccgt catacgcaga
     1681 cgcaagtaat ctttcggtaa taatactttg tcgatttgac ggaatatctc cggctcatgc
     1741 cgctgaaccc atagcaattt aggcgcagta aatccgggca tcatcaggtt gccggtaatc
     1801 actcgtgatt gcggaactct cgcttccagc aaagtgcact cttgcgcaca gcgcccgtcg
     1861 ttccacaaaa tggcagggcg caatacccgt tgttgagcat ccagtaaggt tgctccatgc
     1921 atctggccgg caatacccaa tgctttaacg tcctgcagag aatgctgatc gcccagagct
     1981 ttcattgcgc gatcagttgc ctgccaccac tgttccgggt cttgttccga ccagagtgga
     2041 tgcgggcgcg aaacggtcag cttttccgtt tgcgaatcaa ccacctcacc ctgctcgttg
     2101 agcaaaataa cttttacgcc cgaggtgcca agatctatcc cgatatacat atcgatcgtt
     2161 ccttaaaaaa atgcccggta tcgctaccga taaccgggcc aacggactgc acagttagcc
     2221 gttatttgtc gaacagataa tgatttacca gattttccag ttgctcctgg cgaccactct
     2281 gatgcaccgg agacaaatta tgttcctgag catatttggc taaatctgcc agtgacattt
     2341 ggcctttcag gatttgctgg cccaattcgc tattccagcc ggaataacgc tgcgcgatgc
     2401 gtttatccag ctcgccatct tcaatcatgc gcgctgcaat tttcagcgcc agtgccatcg
     2461 tatccatcgc gccgatatga ccgtaaaaca gatcatattt atcagtactt tgacgacgta
     2521 ctttggcatc gaagttcaga ccaccggtgg tgaaaccgcc tgctttgaga atttcataca
     2581 tcaccagcgc attctcttcc acactgttcg ggaactggtc ggtgtcccag cccagttgcg
     2641 catcgccacg gttggcgtcg acagaaccga acaggccaag cgcaatggcg gtggctattt
     2701 catgatggaa agagtgacct gccagcgtcg cgtggttagc ttcaatgttc agtttaatct
     2761 ctttttccag accaaactgt ttcaggaagc catagaccgt cgcggcatcg taatcatatt
     2821 gatgtttagt cggttcttgc ggtttcggtt cgataagcaa cgtgccctgg aagccgattt
     2881 tatgtttatg ctcaaccacc atctgcataa agcggcccag ttgttcacgc tcctgacgca
     2941 agtcggtatt taacagcgtt tcgtaacctt cacgaccacc ccacaggaca tagttttcac
     3001 cgcccaattt atgggttgct tccatcgctg taacaacttg cgttgccgcc cagctgaaga
     3061 cttcaggatc tgggttcgtc gccgcacccg cgccatagcg agggtttgta aagcagttag
     3121 cggttcccca cagcagcttc acgccgctct cttcttgctt ggctgccagg acatcaacca
     3181 tttgcgcaaa attattgatg tactctttta acgacgcgcc ctcaggggaa acatccacat
     3241 cgtggaagca ataaaatggc acatgtaact tgtggaaaaa ctcaaatgcg acatctgctt
     3301 tacgcttcgc caacgccagt gcctcaccag gctgctgcca cggacgatta aacgccccca
     3361 caccaaacat gtccgccccg ttccagcaga aggtgtgcca gtagcaggcg gcaaaacgca
     3421 agtgctcttc catacgctta cccaacacca gttcgtcggg attgtagtga cggaatgcta
     3481 acgggtttga ggattttgag ccttcataac gaatgcgatc gagctggtca aaataggctt
     3541 gcatat
//
LOCUS       DAESHI010000232         3322 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.235, whole genome shotgun sequence.
ACCESSION   DAESHI010000232 DAESHI010000000
VERSION     DAESHI010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.235"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..320)
                     /gene="fliZ"
                     /locus_tag="KW011_004338"
     CDS             complement(<1..320)
                     /gene="fliZ"
                     /locus_tag="KW011_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HBH7662499.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTV"
     gene            complement(366..1085)
                     /gene="fliA"
                     /locus_tag="KW011_004339"
     CDS             complement(366..1085)
                     /gene="fliA"
                     /locus_tag="KW011_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HBH7662500.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(1250..2824)
                     /locus_tag="KW011_004340"
     CDS             complement(1250..2824)
                     /locus_tag="KW011_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HBH7662501.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALSEINNNLQRIRELSVQATNG
                     TNSDSDLNSIQDEITQRLSEIDRVSNQTQFNGVKVLASDQTMKIQVGANDGETIEIAL
                     DKIDAKTLGLDNFSVALGKVPMSSAVALKSEAAPDLTKVNATDGSVGGAKAFGSNYKN
                     ADVETYFGTGNVQDTKDTTDATGTAGTKVYQVQVEGQTYFVGQDNNTNTNGFTLLKQN
                     STGYEKVQVGGKDVQLANFGGRVTAFVEDNGSATSVDLAAGKMGKALAYNDAPMSVYF
                     GGKNLDVHQVQDTQGNPVPNSFAAKTSDGTYIAVNVDAATGNTSVITDPNGKAVEWAV
                     KNDGSAQAIMREDDKVYTANITNKTATKGAELSASDLKALATTNPLSKLDEALAKVDK
                     LRSSLGAVQNRFDSAITNLGNTVNDLSSARSRIEDADYATEVSNMSRAQILQQAGTSV
                     LAQANQTTQNVLSLLR"
     gene            3079..3322
                     /locus_tag="KW011_004341"
                     /pseudo
     CDS             3079..3322
                     /locus_tag="KW011_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
BASE COUNT          852 a          789 c          713 g          968 t
ORIGIN      
        1 accgtacctg ggctcatttc agtttgctca aaaagaaatt gcttaaagcc gataatatcg
       61 aaaaaatcac tctgcgtttt gcagtgtaaa tcaccacaaa atcggcacaa tgccgcccat
      121 gatttttgtt ccacttgcca gccattttca tcaagcagca tgtccaggcg ggaaatctcg
      181 attttattca ctattttgcc gtcgcgaacg agggtaatgc ggtcgagtaa tttacggcaa
      241 tgcgcgcaat gagtctggct gtgtttaaag tctttaagat agcggcttaa tggccgtctt
      301 ttcaggtgct gcaccatcat taagaactcc tggtagtcaa aattaaagtg cggcatttac
      361 tgacgttata acttacccag tttagtgcgt aaccgtttaa tggcctggct gtgtaactga
      421 ctgacccgcg attccccgac ctccagcacc gcgccaatct ctttgagatt cagctcttcc
      481 tggtaataga gggttaatac cagtttttcg cgctccggca acgtttcgat ggcttccatc
      541 acccgctggc gcagattact gtccagcagt tgttgtagcg ggttttctcg ctgatgatca
      601 tcagtaacca gttcgatgct atcgccgtgc tcttcgcgcc actcatcgta ggagaagagc
      661 tggctgttat tggtgtcgag caacatttgg cgataatcgg caatatcgat ccctaaacgt
      721 tccgctacct cagtttccgt ggcgttgcgg ccaagttcct gctccagttg ccctattgcc
      781 tgtgccactt cacgcgcgtt gcgtcgcacg ctgcgcggca cccagtcacg gctgcgaagt
      841 tcatccagca tagcgccacg gatacgctgc actgcgtaag ttgtaaatgc cgttccttgt
      901 agggcgtcat agcgttcgac ggcattaagt aacccaatgc cgcccgcctg tagcagatcg
      961 tcaagttcca cgctcgcggg cagtcgaacc tgcaggcgca atgcttcgtg acgcaccagc
     1021 gggacataac gctgccacag cgagtgttta tccattacac cttcagcggt atagagtgaa
     1081 ttcacgataa acagccctgc gttatatgag ttatcggcat gattatccgt ttctgcaggt
     1141 tttttaatcg gacgattagt gggtgaaatg aggggttatt taggggttac cggtaaattg
     1201 cggacagaaa aaaccccgcc ggtggcgggg tttgagcgat aagtgtaaat taacgcagca
     1261 gagacagtac gttctgcgtg gtctggttag cctgtgccag aacagaggta cccgcttgtt
     1321 gcaggatctg cgcacgagac atgttagaca cttcggttgc gtagtcagca tcttctatac
     1381 ggctacgggc agaagacagg tcgtttacgg tgttgccaag gttggtgatg gcagagtcga
     1441 aacggttttg tactgcaccc aaagaactgc gcaacttatc aacttttgcc aaagcttcgt
     1501 ctaatttgga taatggattt gtggttgcta aggctttcaa atctgaggca ctgagttcag
     1561 cacctttggt tgccgtctta ttcgtgatat tggctgtata aaccttatca tcttcacgca
     1621 taattgcctg tgcagaacca tcatttttta ctgcccattc aactgcctta ccattaggat
     1681 cagtaataac agacgtgtta cctgtagcgg catctacatt tactgcaatg taggtgccgt
     1741 ctgatgtttt agcagcaaat gaattaggta caggattccc ttgggtatct tgtacttggt
     1801 ggacatctag gttttttccc ccaaaataaa cagacattgg tgcatcatta taagctaatg
     1861 ctttacccat tttacccgca gctaaatcaa ctgatgtggc agaaccatta tcttcaacaa
     1921 atgcagttac acgaccacca aagtttgcta actgaacatc cttaccaccc acctgaactt
     1981 tttcataacc tgtagagttt tgtttcaata atgtaaaacc gttcgtgttg gtattattat
     2041 cttgaccaac aaaataagtc tgcccttcca cctgtacttg ataaactttt gttcctgcag
     2101 taccggtcgc atcagttgta tcctttgtat cttgtacatt accggtacca aaataagttt
     2161 caacatcagc atttttataa ttgctaccga atgctttagc acctcccaca ctaccatcag
     2221 ttgcatttac cttagttaag tcaggagcgg cttcgctctt aagtgcaacc gcagaggaca
     2281 ttggaacttt tcctagtgct acgctaaagt tatcaagccc caaggtttta gcatcaattt
     2341 tatcaagggc aatctcaatg gtttcaccat cgttcgcacc tacttgaatt ttcatagtct
     2401 gatcagaagc cagaactttt acaccattaa attgtgtctg gttagaaaca cgatcaattt
     2461 cactaaggcg ttgtgtaatt tcatcctgaa ttgaattcag gtcggagtca gagtttgtac
     2521 cattagtggc ctgtactgac aattcacgaa tacgctgtaa gttattgttg atttcagaaa
     2581 gcgcaccttc agtggtctgc gcaacagaaa taccatcatt cgcgttacgg gaagcctgag
     2641 tcagaccttt aatatttgct gtaaaacggt tagcaatcgc ctgacctgct gcgtcatctt
     2701 tagcactgtt aatacgcagg ccagaagaga gacgttcaat ggcggagctc agagaagact
     2761 gagatttgtt caggttattc tgggtcaaca gcgacaggct gtttgtatta atgacttgtg
     2821 ccatgatctt ttccttatca attacaactt gatgttattg ggctgttgcc cacggtttct
     2881 caccgtaccc cttgtatcgg cacctgaatt tcgaacttta gaaaatttta gtttctctcc
     2941 tgttttttct taacggctga aatatccgta atatacaact ctactctgcg catcatcttc
     3001 gtgaatcaac tattaaactt tgcgcacaaa ttgccgataa cccgcttatc tactgttttc
     3061 aatcaaaagg aattaggtat ggcttcattt acatctcttg gcgttggttc gaatctgccg
     3121 ctggatacat tgctaaacaa cctgaccatc gctgaaaaaa aacgcttaaa cccaattact
     3181 cagcaacaga gtgacaatac cgcacgtctg acagcctatg gcacattaaa aatgtgcatt
     3241 agaaaaattc cagactgcaa atactgcgtt aaataaagcc gatctgttca aaagcactac
     3301 cgttaccagt agcaatgaag ac
//
LOCUS       DAESHI010000233         3236 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.236, whole genome shotgun sequence.
ACCESSION   DAESHI010000233 DAESHI010000000
VERSION     DAESHI010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.236"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(90..617)
                     /gene="mobB"
                     /locus_tag="KW011_004342"
     CDS             complement(90..617)
                     /gene="mobB"
                     /locus_tag="KW011_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBH7662502.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(599..1183)
                     /gene="mobA"
                     /locus_tag="KW011_004343"
     CDS             complement(599..1183)
                     /gene="mobA"
                     /locus_tag="KW011_004343"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HBH7662503.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1253..1522
                     /locus_tag="KW011_004344"
     CDS             1253..1522
                     /locus_tag="KW011_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HBH7662504.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1599..2585
                     /gene="srkA"
                     /locus_tag="KW011_004345"
     CDS             1599..2585
                     /gene="srkA"
                     /locus_tag="KW011_004345"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HBH7662505.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2602..3228
                     /gene="dsbA"
                     /locus_tag="KW011_004346"
     CDS             2602..3228
                     /gene="dsbA"
                     /locus_tag="KW011_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HBH7662506.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          826 a          781 c          786 g          843 t
ORIGIN      
        1 aaggccatcc tgacggatgg cctttttgca ttggcgcaga aaaaaatgcc ggatgcgaca
       61 ctggcgcgtc ttatccagcc tactcttgct tatccgttct gcttttgcat ccactccacc
      121 acaaaatcag ccagcccctc aacatcatta atatccagta atgcgacatc aagattaagc
      181 ggcacatcac tggctacagc aataacatgc ctgtctatca ctaattcttc aggtcgatgt
      241 ccggctccat cgcgaaacag cacaatcttt gcgatctctt catgcttaaa cccttcgacc
      301 agaatcaaat ccagctttga ggtatccatt cgacttgcga gaaactgtag atccagctct
      361 tcttcgtctg gtttttccgt catcaaggcc catcgttgct ggctggcaac gatggtttgt
      421 gccgcgccag ccttgcgcag ctcatagcta tctttgcctg gcttatcaac atccatatca
      481 tggtgcgtat gcttaatcag ccctggacgg atccctctgg cgcataatgc cgggatcagt
      541 tttttcaaaa gcgtagtttt tccggtacca ctccacgcgg caaaggcgag taacggtatc
      601 atcgtttttc ctgccatcgg gcaagctcct ctggcgtatt cacgttaaca aatgcatctt
      661 tatgatcgct gaaatcaacc gcatggccgc cagccagacg cataaatgcc attacccggc
      721 gttctcctgc ttgcagatat tctagtaata aaggctcaat agcacggttt accagagcaa
      781 tagtcgggtg atcgcgttca ccgtcatgga cccacacgac aggcgcatct ttgcgctgat
      841 gattaagccg ggaagctaaa acatggggaa tgtaaggcgt atcgcacgga caaaacaaaa
      901 accactcacc agcttcctgc tgcattactg aaagcattcc tgccagaggg cctgggtaat
      961 ccgccagtga atcttcaatc actttcagac cgcttgcctg atagatttcc tgatgacgat
     1021 tggcattaac cacgacgtga gagagctgcg tcataagcgc gtcagcgaca tgttgccata
     1081 atggtttgcc gtttaattca agcaatcctt tatctacgcc gcccattcgt ctggctttac
     1141 cgcctgccag cacaacgcct gttatcgtcg tcatcagatt caccgatatc gcctctttta
     1201 ttgtgggatt gaccctgcta acgtgtctgt ctcaagagta aggagcatca ctatgaaatg
     1261 taaacgtctg aatgaagtta ttgaactcct ccagccagcc tggcaaaaag agccagacct
     1321 taacctgttg caatttttgc agaaactggc gaaagagtca ggttttgacg gcgaactggc
     1381 ggatttgacg gatgacattc tgatctatca cctgaaaatg cgtgattccg cgaaagatgc
     1441 ggtgatcccg ggtttgcaga aagattatga agaagacttc aaaacggcgc tgttacgcgc
     1501 tcgcggcgta attaaagagt aaaagcttgt aagcggcgcc accaaaatca tcgtgaaatg
     1561 atatccttca tcattcgtaa tgttttccgg atgatgggat gaacaacagc gcttttactt
     1621 tccagacact acacccggat accatcatgg acgctctgtt tgagcaaggg atccgggtgg
     1681 attccggtct taccccgctt aacagctatg aaaaccgtgt ctatcaattt caggacgaag
     1741 agcgtcgacg ttttgtcgtc aaattttatc gccctgaacg ttggacagcc gatcaaatcc
     1801 tcgaagaaca tcaatttgcg ttgcagctgg taaatgatga agttccggtc gcagcacctg
     1861 tggcctttaa cggtcagact ttattgaatc atcagggatt ttatttcgct gtttttccaa
     1921 gcgtcggtgg tcgccagttc gaagctgata atatcgatca gacggaagcg gttgggcgtt
     1981 atttagggcg tatgcaccag acggggcaca aacagctttt tatccatcgc ccgaccatcg
     2041 gtctgaatga atatctcatt gagccacgca agctgtttga ggacgctaca ctgatacctt
     2101 ccgggttgaa agcggcattc ctgaaagcga cagatgagct gatcgccgcc gttacagcac
     2161 actggcggga agatttcacc gttctgcggc tacatggaga ctgccacgcc gggaatattc
     2221 tctggcgcga tggtccaatg tttgttgatc tggatgatgc acgtaatggt ccagctattc
     2281 aggatttgtg gatgttgctc aatggcgata aagccgagca gcggatgcaa ctggaaacta
     2341 ttattgaagc ttatgaagaa tttggcgagt tcgacaccgc tgaaatcgga ctgattgaac
     2401 ctttacgcgc catgcgtttg gtttattatc ttgcctggct aatgcggcgt tgggctgatc
     2461 ccgcgttccc gaaaaatttc ccgtggttaa ccggggaaga ttactggctg cgacaaacgg
     2521 cgacttttat agaacaggca aaagttctac aagaaccccc tttacaatta acacctatgt
     2581 attaatcgga gagagtagat catgaaaaag atttggctgg cgctggctgg tttagtttta
     2641 gcgtttagcg catcggcggc gcagtatgaa gatggtaaac agtacactac cctggaaaaa
     2701 ccggtagctg gcgcgccgca agtgctggag tttttctctt tcttctgtcc gcactgctat
     2761 cagtttgaag aagttctgca tatttctgat aatgtgaaga aaaaactgcc ggaaggcgtg
     2821 aagatgacta aataccacgt caacttcatg ggtggtgacc tgggcaaaga tctgactcag
     2881 gcatgggctg tggcgatggc gctgggcgtg gaagacaaag tcactgttcc gctgtttgaa
     2941 ggcgtacaga aaacccagac cattcgttca gcatctgata tccgcgatgt atttatcaac
     3001 gcaggtatta aaggtgaaga gtacgacgcg gcgtggaaca gcttcgtggt caaatctctg
     3061 gtcgctcagc aggaaaaagc tgcagctgac gtgcaattgc gtggcgttcc ggcgatgttt
     3121 gttaacggta aatatcagct gaatccgcag ggtatggata ccagcaatat ggatgttttt
     3181 gttcagcagt atgctgatac agtgaaatat ctgtccgaga aaaaataatt caatgt
//
LOCUS       DAESHI010000234         3221 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.237, whole genome shotgun sequence.
ACCESSION   DAESHI010000234 DAESHI010000000
VERSION     DAESHI010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.237"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            8..511
                     /locus_tag="KW011_004347"
     CDS             8..511
                     /locus_tag="KW011_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH7662507.1"
                     /translation="MNNIAPQSPVMRRLTLLDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(557..973)
                     /gene="rraB"
                     /locus_tag="KW011_004348"
     CDS             complement(557..973)
                     /gene="rraB"
                     /locus_tag="KW011_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HBH7662508.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1135..2139
                     /gene="argF"
                     /locus_tag="KW011_004349"
     CDS             1135..2139
                     /gene="argF"
                     /locus_tag="KW011_004349"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBH7662509.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(2211..>3221)
                     /locus_tag="KW011_004350"
     CDS             complement(2211..>3221)
                     /locus_tag="KW011_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBH7662510.1"
                     /translation="DLLEKPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEID
                     DLNAVNNYLLNNKKATDNEINDIKVNLSHILIDSLDDAKVNLTPVIDSILETFLKSPY
                     INDVRILDWCFNKRMQYFGDSEKIKYACSVINHIDFSCDQSKDFSCDQSKIKIAETLF
                     FNLDKEPYKNSRKLQELIWDKLVAYVNDFSLSNQEKSRLILRLFDDVKLLFNEVPVSI
                     LVNDIFLKDFFMKQPDFAKWYFYQLLKCYEGEQLYLNELGYVYGDEEKTKEIVNKLPG
                     YVVKIFEEKMGNELKIRTRMMKILRNGKINIYGYINEEQLGKLNPPEDLRIAIKKLGW
                     NN"
BASE COUNT          893 a          720 c          675 g          933 t
ORIGIN      
        1 ttaaactatg aataacattg cgccgcaatc acctgtaatg cgtcgcctga cgctgctgga
       61 taatcctgct atcgcccgcg tcattcgtca ggtatccgcc gaatacggtc ttaccgctga
      121 taaaggctac accgtcgccg atccgaatct tgacgagctg tatcaagtgt atagtcagcc
      181 tggccatgca tattgggtgg ttgagtacga gggtgaagtg gtcggcggcg gtgggattgc
      241 gccattaacc gggagtgagt cggacatttg cgaactgcaa aagatgtatt ttcttccggc
      301 tattcgcggt aaagggctag cgaaaaaact ggccttaatg gcgatggagc aggcacgaga
      361 gatgggcttc aaacgctgct atctggaaac gaccgctttt ttaaaggaag ccattgcgct
      421 ttatgagcat ttgggctttg agcatatcga ctatgcgctt ggctgcacgg gccatgtcga
      481 ttgtgaagtg cggatgctac gcgaactcta aaaaaatgcc cgctagtatg tgctatcggg
      541 cattttaatc aattaattag tggcgaaccc cgtcatcgtc ttcatcgaca aaatcttcat
      601 cgtcgccatc ttcgccgtta ggatcttcaa agtaagtgcc ccaaccgtcg tactcaacgt
      661 caaatttctc cgccagcgtc atcagttgtt caacctgggc atcgatcaga tcggcattca
      721 acgcgcactc gctgaggatg tcgcagcaaa tcacgatatc accatcttca acttccagct
      781 cttctggatc ggtcacttcg taaccgagtt taaacgcttc aactgccgct ttttccagag
      841 tttccagatc gtctgcggaa agatggtgtt cgatggtgta caacgcgtcc ggatcgctgc
      901 catcttccag taattcttca ataatcaaac gtgtttcttc acgctgttct tccagttgtt
      961 ccgggtttgc catggctcat tcctcttaat gtcctgccga tactcttatt gtcacacacc
     1021 actgacattg cctccacctt tgtgataaag atttatgctt tagacttgca aatgaataat
     1081 catccatata aattgaattt taattcatta aggcgttagc cacaggaggg acctatgtcc
     1141 gggttttatc ataagcattt cctgaaatta ctcgatttca cgccagctga actcaacagc
     1201 ctgctgcagt tagccgcgaa gctgaaagcc gataagaaaa gcggtaaaga agaagccaaa
     1261 ctcactggta aaaacatcgc gctcatcttc gaaaaagact cgacccgtac ccgatgctct
     1321 ttcgaagttg ccgcatatga ccagggggct cgtgttactt atctcggccc aagcggcagc
     1381 caaattggtc ataaagagtc gattaaagac actgcccgcg tgcttggtcg catgtatgac
     1441 ggtattcagt atcgcggcta tggtcaggag attgtcgaaa cactggcgga atacgctggc
     1501 gtgccggtat ggaacggcct gaccaatgag tttcacccca cgcagctgct ggcggatctc
     1561 ctcaccatgc aggagcattt gcccggcaaa acgttcaacg aaatgacgct agtctatgca
     1621 ggtgacgcac gtaacaacat gggcaattcg atgctcgaag ctgcagcgct taccggtctg
     1681 gatttgcgtc tggtcgcgcc acaggcatgc tggccggaag ctgcgctggt tacggaatgc
     1741 cgcgccctgg cacaacaaaa tggcgggaat attacgctga cggaagatgt agctaaagga
     1801 gttgaaggtg ctgactttat ctataccgat gtatgggtgt cgatgggtga agcaaaagag
     1861 aaatgggctg aacgcattgc attgctgcgt gattatcaag tgaacagcaa gatgatgcag
     1921 ttgaccggta acccggaggt caaattcctc cactgcctgc ccgcgtttca tgacgaccaa
     1981 acgacgcttg gcaaaaaaat ggcggaagag tttggcctac atggcggaat ggaagtgact
     2041 gatgaggtct tcgaatctgc cgccagcatt gtatttgatc aggcggaaaa ccgcatgcat
     2101 accatcaaag cggtgatggt cgcgacgctc agtaaataat tgatatcgct ataaagatgc
     2161 cgggaaatac aaaaacatcc cggcgtcaca ttattttatg cagtgatagt tcaattattc
     2221 caccccaatt tttttattgc tatacgtaaa tcttccggag gatttaattt tcctaactgt
     2281 tcttcattaa tatatccata tatattgatt ttgccattcc ggaggatttt catcattctt
     2341 gttctaattt ttaattcatt acccatcttt tcttcaaaaa tcttaacgac ataacccgga
     2401 agtttattta caatttcttt tgttttttct tcatcaccat aaacatatcc taactcatta
     2461 agatacagct gttcgccctc ataacactta agtaattgat agaagtacca cttagcaaaa
     2521 tcaggttgct tcatgaaaaa atccttcaaa aaaatatcat ttactaaaat gctgacaggc
     2581 acttcattaa atagtaactt aacatcatca aataacctta gtattaagcg tgatttttct
     2641 tgattgctta agctaaaatc attgacatat gcaaccaact tatcccaaat caattcctgt
     2701 aatttacggc tatttttata gggttcttta tcgagattga aaaataaggt ctcagctatt
     2761 ttaattttag attgatcgca gctaaaatct ttagattgat cgcagctaaa atcaatatga
     2821 tttatcacgg agcatgcata ctttatcttt tcagaatcac caaaatattg cattctttta
     2881 ttaaaacacc aatccagtat tcttacatca ttaatatatg gggattttaa aaaagtctcc
     2941 agaatcgaat cgatgaccgg tgtaaggtta acttttgcat catctaagga atcaataaga
     3001 atatgagaca ggttaacttt gatgtcatta atttcattgt cagtagcttt cttattatta
     3061 agaagataat tatttactgc gttaaggtca tctatttctt tcagtatccc tttttcacca
     3121 agcaagtatg aaatcgattc attacataca taaggcatta atagcagatg ttctttcctt
     3181 attgctaata atgcattgtt caatggtttt tctaataaat c
//
LOCUS       DAESHI010000235         3216 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.238, whole genome shotgun sequence.
ACCESSION   DAESHI010000235 DAESHI010000000
VERSION     DAESHI010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.238"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..495
                     /locus_tag="KW011_004351"
     CDS             <1..495
                     /locus_tag="KW011_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBH7662511.1"
                     /translation="SRAPFIITPPLFRIEAGDDSSLRIIKTADNLPENKESLFYINVR
                     AIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRSDHSLNIYNP
                     TEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAINDYGGSSGTET
                     RPLQ"
     gene            525..3041
                     /locus_tag="KW011_004352"
     CDS             525..3041
                     /locus_tag="KW011_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBH7662512.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3052..>3216
                     /locus_tag="KW011_004353"
     CDS             3052..>3216
                     /locus_tag="KW011_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBH7662513.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGRE"
BASE COUNT          938 a          771 c          731 g          776 t
ORIGIN      
        1 agccgtgccc catttattat aaccccaccg ctatttcgta ttgaagctgg cgatgactca
       61 tcactgcgaa ttattaaaac agctgataac ctgcctgaaa ataaagagtc gctgttctac
      121 attaatgttc gtgccattcc agcaaagaaa aaatcagatg atgttaatgc taacgagttg
      181 acgctggtat ttaaaacacg gatcaaaatg ttttatcgcc ccgcacacct gaagggacgg
      241 gtaaacgatg cgtggacatc actggaattt aaacgtagtg accattcact caatatatat
      301 aacccaactg aatattacgt cgtatttgcc ggactggcag tcgataaaac cgatctcaca
      361 agcaaaattg aatatatcgc gcccggagaa cataaacagt taccacttcc tgcatctggc
      421 ggaaagaacg tgaaatgggc tgcaatcaat gattatggcg gcagttccgg gacagaaact
      481 cgtccactgc aataacaaat ataaaaaaca caggtcatca gggaatgcca caacgacacc
      541 accagggaca taaacgcaca ccgaaacagt tggcgctcat catcaaacgt tgtttgccga
      601 tggtgctcac tggcagcggc atgctttgca ctaccgctaa cgccgaagag tattatttcg
      661 accccattat gctggaaact acaaaaagtg gtatgcaaac aaccgatctg tcacgttttt
      721 caaaaaaata cgcacaacta ccaggaactt atcaggttga tatctggctg aataaaaaga
      781 aggtttcaca gaaaaaaatt acatttaccg ccaatgcaga gcaacttctg cagccacagt
      841 ttacggtaga acaactacgt gagctgggta ttaaggtgga tgaaatcccg gcgctggctg
      901 aaaaagatga cgatagcgtg atcaactcgc ttgaacaaat cattcccggt acagcagctg
      961 aatttgattt caatcatcag cgacttaatt tgagcattcc ccaaattgca ctgtaccgtg
     1021 atgcaagagg ttacgtctcc ccttctcgtt gggacgatgg tataccaacg ctgtttacca
     1081 actactcgtt tacaggttct gataaccgtt accgccaggg caatcgtagc caacgacagt
     1141 acctgaatat gcaaaatggt gctaattttg gcccctggcg attacgcaac tattccacat
     1201 ggacacgcaa cgatcagaca tcaagctgga ataccatcag tagttattta caacgtgata
     1261 tcaaggcatt gaagtctcag ttgcttctgg gagaaagcgc caccagcggc agtatttttt
     1321 ccagctacac ctttactggc gttcaactcg cttccgacga taatatgttg ccaaacagcc
     1381 agcgcggatt tgccccaacg gtacgcggta tcgcaaacag tagtgcaatc gtgactatca
     1441 ggcaaaatgg ttatgtgatc tatcaaagca acgtgccagc gggtgccttt gaaattaacg
     1501 atctctaccc ctcttccaac agcggcgatt tagaagtcac gattgaagaa agtgacggta
     1561 cgcaacgtcg ctttatccag ccttattctt cattacccat gatgcagcga cctgggcatc
     1621 taaaatatag cgcgaccgct ggacgctatc gcgctgatgc aaacagtgat agcaaagaac
     1681 ccgaatttgc tgaagccacg gcaatatatg gtttgaataa tacttttacg ctgtatggtg
     1741 gcctgctcgg ttctgaagat tattatgcgc tggggatcgg tatcggcggc acacttggcg
     1801 cactgggcgc gttgtcgatg gatatcaaca gagctgacac ccaattcgat aaccagcact
     1861 cttttcatgg ctatcaatgg cgtacgcagt acatcaaaga tatcccggaa accaacacca
     1921 atatcgctgt cagctactat cgctatacca acgatggcta ttttagtttt gatgaagcca
     1981 atacccgtaa ttggaactat aacagtcgcc aaaaaagtga aattcaattc aacatcagcc
     2041 agacaatatt tgatggggta agtctgtatg cctccggttc gcagcaagac tattggggca
     2101 ataacgataa aaacaggaat atctcagttg gggtttccgg tcagcaatgg ggaattggtt
     2161 acagcctgaa ttatcaatac agtcgctaca cggatcaaaa taatgaccgc gcactctctt
     2221 tgaatctcag tattccgtta gaacgctggt taccgcgtag ccgggtttcc tatcagatga
     2281 ccagccagaa agatcgccca acccaacatg aaatgcgtct taatggctca ctgctggatg
     2341 atggtcgcct gagctatagc ctggaacaaa gtctggatga cgataacaac cataacagta
     2401 gcctgaacgc cagttaccgt tcaccttatg gcaccttcag tgccggatac agctacggta
     2461 atgacagcag ccaatacaat tacggcgtta ccggcggcgt ggttatccat actcatggcg
     2521 tgacgctctc gcaatatctg ggcaacgctt ttgcgcttat cgatgctaat ggggcttctg
     2581 gcgtgaggat acaaaactat ccggggattg ctactgatcc ctttggctat gcagtggttc
     2641 cttatctcac gacttaccag gaaaaccgtc tctcggtaga tactacgcag ctgcccgata
     2701 acgtcgatct tgagcaaaca acacagtttg tggtgcccaa cagaggtgca atggtagcgg
     2761 cgcgtttcaa cgccaatatc ggttatcgcg tacttgttac agtcagcgat cgcaacggta
     2821 aaccgttgcc ctttggcgct cttgccagca acgatgagac ggggcaacaa agtatcgtcg
     2881 atgagggcgg catactatat ctctctggga tatcgagtaa atcacaaagc tggactgtac
     2941 gctggggaaa tcaggcagat caacaatgtc agtttgcttt tagtacaccg gattcagaac
     3001 caacaacctc tgtattacaa ggcacggcgc agtgccatta aggataaaaa aatgaaaaga
     3061 gcgcctctta taacaggact tttgttgata tccacatcct gcgcttatgc ctcctcagga
     3121 gggtgtggag ctgacagcac tagcggtgcg acaaattaca gcagtgtggt tgatgatgtt
     3181 acggtgaacc agacagataa cgtgacagga cgggag
//
LOCUS       DAESHI010000236         3172 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.239, whole genome shotgun sequence.
ACCESSION   DAESHI010000236 DAESHI010000000
VERSION     DAESHI010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.239"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(318..1763)
                     /locus_tag="KW011_004354"
                     /pseudo
     CDS             complement(318..1763)
                     /locus_tag="KW011_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            complement(1915..2514)
                     /locus_tag="KW011_004355"
     CDS             complement(1915..2514)
                     /locus_tag="KW011_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HBH7662514.1"
                     /translation="MRKVCAAILSAAICLSVSGAPAWASEHQSTLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDALGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMVGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVTIDLAYEGSGSGDWRTDAFIVGIGYRI"
     gene            complement(2582..>3172)
                     /locus_tag="KW011_004356"
     CDS             complement(2582..>3172)
                     /locus_tag="KW011_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH7662515.1"
                     /translation="PTITSGGNPPAFSLTPDGKLTAKNADISGSVNANAGTLNNVTIN
                     ENCQIKGKLSANQIEGDIVKTVGKAFPRDSRAPERWPSGTITVRIYDDQPFDRQIVIP
                     AVAFSGAKHERENNDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMPAGRGHMTL
                     EFSVSAWLVNNWYPTASISDLLVVVMKKATAGISIS"
BASE COUNT          620 a          899 c          829 g          824 t
ORIGIN      
        1 aaatacggaa acttgcctgc cgcagtagta agtccggcca gcgccgtcag ggtggcatct
       61 ttcggttgct tacccgcaag cgcgttagtc atggtggtcg caaaattcgg gtcgttgccc
      121 agcgccgcag ccagctcgtt cagcgtgttc agtgcatcag gtgacgaatc tacaagggcg
      181 gcaatcgcag ccataacgaa agccgtgctt gcgatttggg tattattcgt tccctgtcgc
      241 gcagttggtg ttgttggcgt tccggtcagt gcaggactat ttaagggcgc tttcttgttc
      301 gtttcaccca ttaccgcctt aaccgctttt ggcgttgccg ccagtgactc ggaagtgctg
      361 ttggtcgcac tgctgagctg tactatcccc tttttcgtcg tgctcgcatc ctccagcgcc
      421 acggcggatg caatatcctc tgcccgtttt gctgctgtct cggcgcgcgt tgccgcggat
      481 tcagcagcaa ctttgctctg agatgcagcc gtcgcactgc ctgccgcctc tgatgctttc
      541 gttgttgctg tcgtggcact acctttcgct gctgacgctt gtctggtcgc ctcatctttt
      601 gaagcagacg cagatgatgc cgatgacgcc gctgaactgg ctgacgatgc ggctgctgcc
      661 ttagaggaag cagcattgtc tgctgaagtc tttgcatttg tttcagaggt ttttgctgca
      721 gaagcagacc tcgctgctgc agtggcttgc tcagtggctt tgccagcctt cgttgtggct
      781 gttgaagcgg atgatgcggc gctttctgcc gattttccgg cggcggtggc actggctgag
      841 gcctgcccgg cacttgttga cgcggcactg gcagataatg cagccgctgt ttttgaacct
      901 gccgcagctg aggcgctctg tcccgctgct gtttcagaag acttagcgtt cgtctcggac
      961 gtttttgccg ccttcgcgga atttcctgcc gccgttgccg aggaagctgc actactggca
     1021 cttgatgatg cattcgtttc tgaagatttc gctgcctctt ttgaggccgc cgcaccccgt
     1081 gccgaggtgg cagcttctga cgccttcgtg gtcgctgtgg atgcagaagt ggctgccgat
     1141 ttttgtgatg ctgcggcatt cgtttctgac gttttcgcgg cactggcgct ggtagctgcc
     1201 gcgctttttg atgactctgc agcagcactt tttgctgctt cacgggcctt tgtcgatgcc
     1261 gttcctgcgc tggaagacgc tgactgagcc gacgacgcgg cctgtccggc tgacgtgctg
     1321 gcggcacgtg ctgaggctgc agcatcggtt gcatgagttg ccgcctcgct ggctgatttt
     1381 ttcgcggctg ccgtattctg tgcaaccgcg gaggcgttac gtgacacctc ttccaccatc
     1441 tgctcaaagc ggcgcagtgc ctccggtcgg acatcatcct ccgtcatggc accgagaaaa
     1501 tcattcagcg tacctggtct ggaaccttca tagacggtaa tggtcccggc atgtgaaggc
     1561 ggaaaacctt caaccagcag ggtgacgctg tactgaccat gctcaacatc catgctgtaa
     1621 cgtccggctt catccggatt ttcagaggcc accgtgttca ccaccatcgt gctgctggtt
     1681 cgtctggcct tcagcacaat ggtgcagttc tgtactggtt ttcctgtgcc atctttaagc
     1741 acgccagaaa tttttactgt catacttttc caccaataaa aaaagcccgc agcagtgacg
     1801 ccacgggctt caggacagtg taactttacg tttcctcaaa cgcagttcac cccataaggt
     1861 ggatgaacct gcgtatcata acaatattta cagaagataa atcggcgtct gttgtcagat
     1921 acggtatccg ataccaacaa taaatgcatc cgttcgccag tcaccactac cggaaccttc
     1981 ataagcaagg tcaatggtca cggattcggt cgggttaaac tgcacgccag ccccccacgc
     2041 cagagacgtg ttgctgtggc gaccgtcatc acttccggtc agcacatcgt gcgttttccc
     2101 cttgttgtca gttacgcgga gataatcccc ggagaaagtc gacacacggc tgtaagccac
     2161 acccaccatc gcatacgcgc tgaaccattc attcacgcgt acagacggcc ccgccatcac
     2221 gctgaaccag cggttacgca cggaatcttc atgccagcgg gtatcgctgt agtgcgtttt
     2281 ttgctcatcc tcagcattgg cataactgaa ggacgtaatc agccccagcg cgtccgtaaa
     2341 ctcataacgg tatttcacgt taatcccgtt cagattatcg ctgccgggag cgttcgtacg
     2401 ggcatgaaga taccccgcgc tcagtgtgga ctgatgttca gacgcccatg caggcgcacc
     2461 ggatacggac agacagatgg ctgcggacaa aatggctgca caaactttac gcataattac
     2521 ctctcgcttt tctgcaataa aaaaggcgcc atttctggcg cccgtatatg ggttataaaa
     2581 ttcagctgat actgatgcct gcggtggctt tcttcatcac cacaaccagc aaatcgctga
     2641 tacttgctgt gggataccag ttattcacca gccatgctga caccgaaaac tccagcgtca
     2701 tgtggccgcg accagctggc atatcaataa caccactgta aatcagcgta ttatccagcg
     2761 cggtacggtt ataaatttca gcaccgtttt tccgtactat caggcggcat gacgaataaa
     2821 tatcgttatt ctcccgctca tgtttagcgc cgctgaatgc caccgccgga ataacaattt
     2881 gcctgtcaaa aggctgatcg tcataaatcc tgacggtaat ggtccctgat ggccaccgct
     2941 ccggtgcccg ggagtcccgc ggaaaagcct tacccactgt tttgactata tcgccttcaa
     3001 tctggttggc tgacagtttc cccttaatct gacagttctc attaattgtg acattgttga
     3061 gcgtcccggc gttcgcattc acactgccac tgatatctgc atttttagcg gtcagttttc
     3121 cgtccggtgt cagggaaaat gccggaggat taccgccgct ggtaatggtg gg
//
LOCUS       DAESHI010000237         3047 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.240, whole genome shotgun sequence.
ACCESSION   DAESHI010000237 DAESHI010000000
VERSION     DAESHI010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.240"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..400)
                     /locus_tag="KW011_004357"
     CDS             complement(<1..400)
                     /locus_tag="KW011_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HBH7662516.1"
                     /translation="METNKKKLTRILKKVLPTIQPPKIQKTSEWISNGVVKFVDGPNM
                     GLDWVPFSFQKEPMDIAQERSTKKIVLQSCSQLLKTTVLQSIAFNLMANDPCNFAFGS
                     SSESEVKKFKDGKFLPAIETSSVLKPLVTDK"
     gene            complement(315..722)
                     /locus_tag="KW011_004358"
     CDS             complement(315..722)
                     /locus_tag="KW011_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBH7662517.1"
                     /translation="MRDTNTKEQIEQERLKKLSAERQLAELELAEKNGLVVSTEYIEQ
                     VLTEYLFQVKTAVRAIPSKTYLELFAQSDAKDLRDVLRTHIDKTLYQLGSMEFELPDD
                     MEVIEDGNKQEEINEDTEESTTDDTTAEDTENE"
     gene            896..1459
                     /locus_tag="KW011_004359"
     CDS             896..1459
                     /locus_tag="KW011_004359"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662518.1"
                     /translation="MSDRNNDFSLLVKLLNGFLKKDEIKKRLYSFVKKETNDWEKWLQ
                     IEFAHYLTSKRKYELHREVTIYLDPIDYPDSKLAKVDLILREKSTPSDDYIFIEFKCT
                     KGVVPLVRGLNADKNKISAIRQCNYKMRNYLGVGFHLFCEPDDIEFMEKYVTEELKGS
                     YAVFKLCECPVRYNCQCEFNEIGVVLF"
     gene            complement(1605..1907)
                     /locus_tag="KW011_004360"
     CDS             complement(1605..1907)
                     /locus_tag="KW011_004360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662519.1"
                     /translation="MEKLILLLLLPLTTSAAIPDFKCWDIHNDSFSTDMSFYVTRYLG
                     KMPPPYGAFHYETAPDEKFYRELVITNEAFDDGYNYGSRYLNKEGTLYDDIKCKRL"
     gene            complement(2127..2567)
                     /locus_tag="KW011_004361"
     CDS             complement(2127..2567)
                     /locus_tag="KW011_004361"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662520.1"
                     /translation="MKITVDNISEMFAGTKELLIGGLITGIVGVLILTRVSGYEYVKV
                     PLMLVMIFLLTVVPGFIAMLFSPLTKVFAKIFDIKSSNILCRIFSFLYFILYMAITFL
                     VTNFIFTTAMSLHGGDGGLRACFAIGCFVVAVEQTRKFYSLLIK"
BASE COUNT          938 a          605 c          499 g         1005 t
ORIGIN      
        1 tcttatcggt tactaacggc tttagtactg aactggtttc aatagctgga agaaatttac
       61 catccttgaa tttcttcact tctgattcag aactactacc aaaagcaaag ttacagggat
      121 cattagccat taggttaaat gcaattgatt gtagtactgt agttttcaaa agctgggaac
      181 aagattgaag tacaatcttt ttagtacttc gctcttgagc tatatccatt ggttcttttt
      241 ggaaactaaa cggaacccaa tcaaggccca tattcggccc gtccacaaat ttaactacac
      301 cattactaat ccactcactc gttttctgta tcttcggcgg ttgtatcgtc ggtagtactt
      361 tcttcagtat cctcgttaat ttcttcttgt ttgtttccat cctctattac ttccatgtca
      421 tcgggtaact caaattccat tgagcctaat tggtataatg ttttatcaat atgcgttctt
      481 aatacatcgc gtaaatcctt tgcatctgat tgtgcaaata attcaagata agtttttgat
      541 ggtattgcac gcactgctgt tttgacctgg aatagatatt ccgttagtac ctgttctata
      601 tattcagtac tgaccaccag cccattcttt tctgctaatt ctaattctgc taactgccgt
      661 tctgctgaaa gcttcttcaa acgctcttgt tcaatttgtt cttttgtatt agtatcacgc
      721 aacggcttta gtacattatc tacaatccaa cttcttgcgt ttgaatcagt gtctgtcggc
      781 attccctttt ctacccaagt tcgaactgta gattcatcgt atccgtactg tctcgctaat
      841 gaccttaatg agatatccat atttgttcct ttttatttta tttatagggt ttacaatgtc
      901 agaccgtaac aatgattttt ctctgttagt aaaattgctg aacggatttt tgaagaaaga
      961 tgagataaaa aaaagactat attctttcgt gaaaaaagaa actaatgatt gggaaaagtg
     1021 gctgcaaata gaatttgctc attatctaac ttcaaaaagg aagtacgagc tacatcgtga
     1081 agttacgata tacctcgatc caatagatta tcctgatagt aaacttgcta aagtagattt
     1141 gatactgcgt gaaaaatcca caccctctga tgattatatt tttattgagt ttaaatgtac
     1201 taaaggtgta gtaccattag ttaggggctt aaatgctgac aaaaataaaa ttagtgctat
     1261 aagacagtgt aactacaaaa tgcgtaatta tcttggtgtc ggatttcatc ttttttgtga
     1321 accagatgat atcgagttta tggaaaagta cgtaactgaa gaacttaaag gctcttacgc
     1381 cgtgtttaaa ttatgcgagt gtccagtaag atataattgc caatgtgaat ttaatgaaat
     1441 tggtgtggta cttttttaaa tgcggtgcgg gtatgatcat tttgttcaca cacaattgat
     1501 taaacccgct gccgaaaacc ctcgatagta ccgccagtat ggagggaccc attgaatgca
     1561 atggttaata tactttgtaa gctatataca atgaatcatg agtatcaaag ccgcttacat
     1621 ttaatatcat cgtacaaagt tccttctttg tttagatatc tcgaaccgta attatatcca
     1681 tcatcaaatg cttcattggt tattaccagt tccctgtaaa atttctcatc aggtgctgtt
     1741 tcataatgaa atgcaccata aggcggcggc attttaccga gatatcttgt aacgtagaaa
     1801 ctcatgtcag tactaaatga atcgttatga atatcccagc atttaaagtc tggaatagct
     1861 gcacttgttg ttaaaggtaa taataatagc aatatcaatt tttccataag agataccaat
     1921 gcaaatgatt taattgcatg ataacaccta caaagtctca ataccaatta acaagcgata
     1981 tggctgattt tgtttcaatt acgtaaaata atttaaaata tcgttaatta aaacaattgt
     2041 gatgaaatgc tcaccttgta atttgttgcg tatttgatga aagtacttac ccttcatcaa
     2101 atacacattt ctttagtact tgccctttac ttgataagca aactatagaa ttttcttgtc
     2161 tgctcaactg caacgacaaa acagccaatt gcaaaacatg ctctcaaccc accatccccg
     2221 ccgtgtaatg acattgcggt agtgaatata aaattagtca ccaagaaagt aatagccata
     2281 tacaagatga aatatagaaa tgagaaaatg cggcaaagta tattactgct tttaatatca
     2341 aatatctttg caaatacttt cgttagtggt gaaaaaagca tagcaataaa accaggaact
     2401 acagttagta agaatatcat tactaacatt aaaggtactt taacgtattc ataaccacta
     2461 acacgagtta aaataagtac tccgacgata cctgttatta agccaccgat tagtagttct
     2521 ttagttcctg caaacatttc agagatatta tcgactgtaa ttttcattct actaactatt
     2581 ccttcataaa aagttcactc cgatcttatc accgcataaa gaaaaagccc tgtagattct
     2641 caccatacag cctaagcact aaacttttcg gattacttcg atactacctg ccattgtcca
     2701 tcatgccgga tgatctcata ctggtactta ccatcacacc gtacatcaat acgtcctgta
     2761 ccattctcag tactatccat agtcaccata tcgcatgggt acttaccctt gattagtgac
     2821 tgtgcgaaat ccatagcgtt ctggttctca ttgtccgtgt actgtggctg gggtgcatcc
     2881 tcacctgcaa tgttgaagag taccccagcc agtactaaca aaatcaccat tacctttccc
     2941 atacgtaccc tatagattgt cgaggatatg ggatggtgaa ttgacccctt ctaaccaatc
     3001 gtgaacacta tcggtctgat gtggtactac cttacttaca tgctctc
//
LOCUS       DAESHI010000238         3045 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.241, whole genome shotgun sequence.
ACCESSION   DAESHI010000238 DAESHI010000000
VERSION     DAESHI010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.241"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..172
                     /locus_tag="KW011_004362"
     CDS             <1..172
                     /locus_tag="KW011_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662521.1"
                     /translation="AKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAV
                     DAPTETCFMHYE"
     gene            185..1036
                     /gene="hyi"
                     /locus_tag="KW011_004363"
     CDS             185..1036
                     /gene="hyi"
                     /locus_tag="KW011_004363"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HBH7662522.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYFLNRVVATGKVMKPVLAQR
                     QKVVSPP"
     gene            955..2133
                     /gene="allB"
                     /locus_tag="KW011_004364"
                     /pseudo
     CDS             955..2133
                     /gene="allB"
                     /locus_tag="KW011_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            2190..>3045
                     /locus_tag="KW011_004365"
     CDS             2190..>3045
                     /locus_tag="KW011_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HBH7662523.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATG"
BASE COUNT          695 a          710 c          836 g          804 t
ORIGIN      
        1 ggcgaaagcc ttaatggcgc aatatcgggt accggtagtc gtggaagtta ttcttgagcg
       61 tgtgaccaat atttcgatgg gcagcgaact ggataacgtc atggaatttg aagatatcgc
      121 cgataacgca gtggacgcac cgactgaaac ctgcttcatg cactatgaat aagggagata
      181 aataatgtta cgtttctctg ctaatttatc gatgttattt ggtgaatatg attttctcgc
      241 ccgttttgag aaagctgcgc agtgtggttt tcgcggcgtt gaatttatgt ttccttatga
      301 ctacgacatt gaagaattaa aacaggtgct ggcgagtaat aaactcgaac atacgctgca
      361 caatctaccg gcgggtgact gggcggcggg ggagcgcggt attgcctgta ttcctggccg
      421 tgaagaagag tttcgggatg gcgtagcagc agcgattcgt tatgcccgtg cgctggggaa
      481 taaaaaaatt aactgtctgg tcggtaaaac gccggctggt ttcagcagtg aacagattca
      541 cgcaacgctt gtagaaaacc tgcgttatgc cgcgaatatg ctgatgaaag aagatatttt
      601 attactgatt gaacctatta accattttga tattcctggt ttccatctca ccggaactcg
      661 gcaggcgctg aaattgattg atgatgttgg ttgctgcaat ttgaaaattc agtatgacat
      721 ttatcacatg cagcggatgg aaggtgaatt aaccaacacc atgactcagt gggccgataa
      781 aattggtcac ctgcaaattg ctgataatcc gcatcgcggc gaaccgggga ccggagaaat
      841 taattatgat tatctcttta aggtaatcga aaattctgac tacaacggtt gggttgggtg
      901 tgaatataaa ccccaaacca ccacggaagc cggtttacgc tggatggatc cgtactttct
      961 gaaccgggtc gtagccactg ggaaggttat gaaaccggta ctcgcgcagc ggcaaaaggt
     1021 ggtatcacca ccatgatcga aatgccgctc aaccagctgc ctgcaacggt tgaccgcgct
     1081 tcaattgaac tgaagttcga tgccgctaaa ggcaagctga ctatcgatgc ggcacaactc
     1141 ggtggcctgg tgtcttacaa catcgaccgt ctgcatgagc tggatgaagt gggcgttgtc
     1201 ggcttcaaat gcttcgttgc gacctgtggc gatcgcggta tcgacaacga cttccgtgat
     1261 gtaaacgact ggcagttctt caaaggtgcg cagaagctgg gcgaactggg gcagccggtg
     1321 ttggtgcact gcgaaaacgc gctgatctgt gacgcactgg gcgaagaagc gaaacgtgaa
     1381 ggtcgcgtaa ctgcccatga ctatgtggct tcgcgtccgg tatttaccga agtggaagcg
     1441 attcgccgcg tgctgtatct ggcgaaagtt gccggttgcc gtctgcacgt ttgccatatc
     1501 agcagcccgg aaagtgttga agaagtgact cgtgcacgtc aggaaggtca ggatgttacc
     1561 tgtgaatcct gcccgcatta ctttgtactg gataccgatc agttcgaaga aatcggtact
     1621 ctggcgaagt gttcaccgcc gatccgcgat ctggaaaacc agaaaggcat gtgggaaaaa
     1681 ctgtttaacg gtgaaatcga ctgcctggtt tccgaccact caccatgccc tccggaaatg
     1741 aaagccggca acatcatgga agcatggggc ggtattgccg gtctgcaaaa ctgtatggac
     1801 gtgatgttcg atgaagcggt acagaaacgc ggaatgtctc tgccaatgtt cggcaaatta
     1861 atggcgacta acgcagcaga tattttcggt ctgcagcaaa aaggccgtat cgccccagga
     1921 aaagatgccg acttcgtctt cattcagccg aatagcagct atgttcttac caatgacgat
     1981 ctggaatatc gccacaaagt cagcccgtat gttggccgta ccattggcgc gcgtatcacg
     2041 aaaaccatct tacgtggtga tgtgatttac gacattgaac agggcttccc tgttgcgccg
     2101 aaaggtcaat ttatccttaa acatcagcag taatctggcc cctgcaatgc ccgtccttgc
     2161 ggcgggcatt ctccggttaa ggtgtgttta tgttcaattt tgcagtcagc cgcgaaagcc
     2221 tgttatcagg atttcagtgg tttttcttta ttttttgcaa cacggttgtg gttcctccta
     2281 cgctactttc tgcttttcag ttgccgcaaa gtagcctgct tacgctcacg caatatgctt
     2341 ttcttgctac cgcactggcc tgcttcgctc aggcgttctg cggtcatcgt cgcgctatta
     2401 tggaagggcc gggtggcctg tggtggggaa ccatccttac tatcaccctt ggcgaagcat
     2461 cgcgcgggac accgatcaac gatatcgcta ccagtctggc ggtggggatt gcactctccg
     2521 gcgtgctgac gatgttgatt ggttttagcg gattaggcca tcgcctggca cggttattta
     2581 cgccgtcggt gatggtcttg tttatgttga tgctgggcgc acagctgacc actatctttt
     2641 tcaaaggtat gctcgggctg ccgtttggca tagccgaccc atattttaaa attcagttac
     2701 caccgttcgc gctctcggtg gcggtgatgt gcctggtact ggcgatgatt atctttctgc
     2761 cgcaacgttt tgcccgttat ggcctgctgg tcggcaccat aaccggctgg ttgttgtggt
     2821 acttttgctt tccttcttcg cattcgctct ccggtgagtt gcactggcag tggttcccgc
     2881 tcggcagtgg cggtgctttg tcgccgggaa ttattctgac ggcggtgatt acaggtctgg
     2941 taaatatcag caatacctac ggtgcgattc ggggcacgga tgttttttat ccgcagcagg
     3001 gcgcggggaa tacgcgttat cgtcgcagct ttgtggcgac cggat
//
LOCUS       DAESHI010000239         2992 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.242, whole genome shotgun sequence.
ACCESSION   DAESHI010000239 DAESHI010000000
VERSION     DAESHI010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.242"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            18..1076
                     /locus_tag="KW011_004366"
     CDS             18..1076
                     /locus_tag="KW011_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBH7662524.1"
                     /translation="MAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTV
                     FTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACH
                     SLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFR
                     DHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKS
                     SEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDH
                     LVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKK
                     SFGWYAEVIKTRGLSLKK"
     gene            1162..2778
                     /gene="bglH"
                     /locus_tag="KW011_004367"
     CDS             1162..2778
                     /gene="bglH"
                     /locus_tag="KW011_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HBH7662525.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2805..>2992
                     /locus_tag="KW011_004368"
     CDS             2805..>2992
                     /locus_tag="KW011_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662526.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVS"
BASE COUNT          838 a          632 c          744 g          778 t
ORIGIN      
        1 atcggctgtt tgatgaaatg gcgcaggcgg ggatcaggcc gctggtaacg ttatcccatt
       61 acgaaatgcc atatgggctg gtgaaaaact acggcggttg ggctaatcgg gcggtcatcg
      121 atcacttcga gcattacgcc cgcacggtct ttacccgcta ccaacataaa gtagcgttat
      181 ggctgacgtt taatgaaatc aatatgtcgt tacacgcgcc attcacgggc gtggggctgg
      241 cagaagagag tggcgaggcg gaagtttatc aggcgatcca tcatcaactg gttgccagtg
      301 cgcgggcagt taaagcctgt catagcctga tccccgaagc gaaaatcggc aatatgcttc
      361 tcggtgggct ggtttacccc ctcacctgcc agccacagga tatgttgcag gccatggaag
      421 agaaccggcg ctggatgttc tttggtgatg ttcaggcgcg tggccagtat cccggctata
      481 tgcagcgttt cttccgcgac cacaatatca ccattgagat gactgaaagt gacgcagaag
      541 atttaaaaca tactgtagat ttcatctctt ttagttatta catgactggt tgtgtttccc
      601 acgacgaaag cattaataaa aatgcgcagg gcaacatact gaatatgatc cccaatccgc
      661 atctgaaaag ttcagagtgg gggtggcaaa ttgatccggt tggattacgg gttctgttaa
      721 atacgctttg ggatcgttat caaaaaccgt tatttattgt cgagaacgga ttaggcgcaa
      781 aagacagcgt tgaagcggat ggttcgatac aggacgatta tcgaattgcc tatttaaacg
      841 atcacctggt acaggtaaat gaagcgattg ccgatggtgt ggatattatg gagtacacca
      901 gttgggggcc aattgattta gtcagtgcat ctcattcaca aatgtctaag cgctacggct
      961 ttatttatgt ggatcgtgat gataatggcg aaggaagcct cacaagaacg cgtaagaaaa
     1021 gcttcggatg gtatgcagaa gtgatcaaaa cgcgggggct gtcattaaaa aaataaccaa
     1081 taaagcacct taattatcgt cgcattcaga acagtctgga tgcgatacgt taattctttc
     1141 tttgcaccat aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta
     1201 atggcaccgt tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc
     1261 cttgagttat tagaaaaagc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat
     1321 gaagagaaga aaaaatatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa
     1381 gggtatgccg ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc
     1441 aaaaatgaag agaaaaatgc cagtgagaca ggttcgattt attcttccat gactctgaaa
     1501 gatttcagta agtttgtgaa agatgaaatt ggctttagtt acaacggcta ttaccgttct
     1561 ggttggggga ccgcctctca tggttcacct aaatcatggg cgattggttc tctgggccgc
     1621 tttggtaacg aatactccgg ctggtttgat ttgcagttaa aacaacgtgt ctacaacgaa
     1681 aacggcaaac gggttgatgc cgttgtgatg atggatggta acgttggtca gcagtactct
     1741 accggctggt ttggcgataa cgccggtggc gagaacttta tgcagttctc cgatatgtac
     1801 gttaccacca aaggtttcct gccctttgcg ccagaggctg atttctgggt gggtaaacac
     1861 ggtgcgccga aaattgaaat ccagatgctg gactggaaaa cgcagcgtac tgatgccgca
     1921 gcgggtgtag gtctggaaaa ctggaaagtc ggtccgggta aaattgatat cgcgctggtt
     1981 cgcgaagatg tcgatgatta cgatcgcagc ctgcaaaaca aacagcagat taataccaat
     2041 actattgatt tacgctataa agatatcccg ttatgggata aagcgacctt aatggtgagt
     2101 ggtcgttatg tcacggcaaa cgaaagcgca tcggaaaaag ataatcagga taataacggg
     2161 tattatgact ggaaagatac ctggatgttc ggcacatctt taacgcagaa atttgataaa
     2221 ggtggcttca acgaattctc cttcctggtt gcgaataact ctatcgccag taactttggc
     2281 cgttatgctg gcgcaagtcc atttaccacc tttaatggtc gttattatgg tgatcacacc
     2341 ggcggaacag cagttcgtct gacctcgcag ggcgaagcct atatcggcga tcattttatt
     2401 gtggctaacg cgattgttta ctccttcggt aacgatattt atagctacga aacaggcgca
     2461 cactctgatt tcgaatctat tcgtgcggtt gttcgcccgg cctatatttg ggaccaatat
     2521 aaccagacag gtgttgaact gggctatttc acccagcaaa acaaagatgc gaatagtaat
     2581 aaatttaatg agtctggtta taaaaccacg ctcttccata cctttaaagt caataccagt
     2641 atgttgacct cgcgtccgga aattcgtttc tacgccacgt atatcaaagc tctggaaaac
     2701 gaactggatg gcttcacctt tgaagacaat aaagacgacc agtttgctgt cggtgcgcag
     2761 gctgaaatct ggtggtaatg gaacttaatg aaaggaatat tacgatgaat ataaaaattg
     2821 ctgctttaac gctggctatt gccagcggta tttctgctca gtgggccata gcagcggata
     2881 tgccagccag cccggcaccc actattccgg ttaaacagta tgtgactcag gtcaatgccg
     2941 ataacagcgt gacctttcgc tactttgccc ctggggcaaa aaacgtctcc tt
//
LOCUS       DAESHI010000240         2938 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.243, whole genome shotgun sequence.
ACCESSION   DAESHI010000240 DAESHI010000000
VERSION     DAESHI010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.243"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            37..264
                     /locus_tag="KW011_004369"
     CDS             37..264
                     /locus_tag="KW011_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBH7662527.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(265..639)
                     /locus_tag="KW011_004370"
     CDS             complement(265..639)
                     /locus_tag="KW011_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HBH7662528.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="KW011_004371"
                     /pseudo
     CDS             complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="KW011_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2099..2674)
                     /locus_tag="KW011_004372"
     CDS             complement(2099..2674)
                     /locus_tag="KW011_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH7662529.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLVNHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          761 a          695 c          689 g          793 t
ORIGIN      
        1 cgttatgccg gtattttttt acggaagagg tatcaaatgc gtttaattgt ggtgagcatc
       61 atggtgacct tgctgagtgg ctgtggcagc atcattagcc gcactatacc ggggcagggg
      121 cacggcaacc aatattatcc cggtgtgcaa tgggatgtgc gtgactccgc ttggcgttat
      181 gtcacaatcc ttgatctgcc attctccctg gtttttgata ctttactgct gccgatcgac
      241 actcatcatg gcccgtatga gtgattaacg ttcatcccac tcatcagctg cagcgtgtcc
      301 ttcctcggta tccagcggtg gctcaagctg aaattccccc tcgtcccatt catgtaatgt
      361 attctcttcc tgccactcct ggcggatctc tatctcatca tagtcgccat caaaaacact
      421 ttgcgcggct tcaccgctaa gcataggtaa acattcacct tcttcccctt cgtcggcaaa
      481 aaactcaact tgccacatga tgtcgccgtc ctgcaaaacg tatttttggg cattgaactg
      541 ttgcacattc gcatcttcgg cgtcgatgcc ggggttgtct gcaagaaatt cttcgcgtgc
      601 agcgtcaatg gcttcttcaa gcgttgcgta catggtcatc acgattctcc ctttgagttg
      661 atgaggtttc agggaaaagg atagctgatt ctccgctttt gcaagtatga aaggcgaaaa
      721 atcagttcga tacctggggt attcgacgac gacgtagact gttccatgaa tagactgcgt
      781 tgaataacac tacgccagcg gtgacgagaa ataccgctct gaaaccgtag ttcgctgaaa
      841 tcgctgctcc catcaatggt ccggtaacgt tgccaatatc acgaaacgat tggttatagc
      901 tgaagatacg tccggcgatt tggttgctcg agttataaac taacagtgtc tgtacggcgg
      961 ggagtagtgc accatcggcg gcaccgagca aaaaacgtaa aatcccaagt tgcaatggcg
     1021 tctgaacgta agacattggg atcaacagca gtacagaaaa gatcagcgcc gtaatcagga
     1081 tcttttcggg tccgattcga tcgccaagtt tgccgagtcg tggtgcactt agcagagccg
     1141 ccacgcctgg caccgaggcg atcatgccac tgataaaggc gacgttactg acgttacccg
     1201 ccagttcgcg gacatacagc gtcagaatgg gggcaattga gcccgtcgcc acctggatga
     1261 ttaacgtagt gacaaacagg ctgagtacca gtttcgggtt tttaagtgat gtcaccactt
     1321 cccgcatgtg cagcatctct tttttgctga tcggctggaa tttttctctg atgcaaaaca
     1381 gggtgacgaa aaagcagagt atgagcacac tggcggtaat aaagaatacc ggacgtaagc
     1441 cgtagctatc ggcgagcagg ccgccagcca ttgggccgag caacgcacca ctaacgccgc
     1501 ctgtggagag cgtacccagc gcccagccgc ttttattacg cggtacttgt gtggcgataa
     1561 gagcattagc gttggggaca aatccgccaa gtaacccaag aagcgcccgc aggatcaaaa
     1621 actgccagat attttgtgcc agccccatca acaccatcac gatgcccatg ccgagggcag
     1681 agcgtaatag catgagtttt cggcctttac ggtcggcgag tccaccccaa aacggtgagg
     1741 cgatggccga aaataaaaat gtaatgctca tatatgctat cttaatgcac tacctgataa
     1801 gtgagcctat acaggttatt actgtatgtt tatacagtat tattggtaga agactgtgta
     1861 gatttggtat agagatgaat agatactatt aaggtaactt attgaaatca ataaagattc
     1921 ttgtgcagtc tgaatagata acttagcgtt atgtctagtc gtttccctct ccattcctaa
     1981 aaagtgctgt atactggcct atacaacgct ataaagacct ctacatagcg ccttgatagc
     2041 tatgtcacag gtacaaaaaa gccccctata aaggaggctg tgtagtctat taagggtctt
     2101 acagggtctt ggtccagtgt ctgctaacag tccttacaga gaggttaaga accttagcta
     2161 cctgagactg agatagtccc ctttccttgt actctaagac cgctctagtt gtgtcactcg
     2221 ctataggtct tccaagtacc ttaccttgaa ctctagcacg ctctagaccc tgtttagtgc
     2281 gctctctgat acggtttctt tcaaactctg caaaggtact aaatagctgt agaaccaact
     2341 taccctctga gcttgaaagg tctttaacgg gtaaatctaa acagatgagc ttcacaccct
     2401 tatcattaag aagggtcact gtattctgta ggtcgatgtt atcccttccc agacggtcaa
     2461 gtttaagaac aacaagggtg tcaccttctt ctagcttatg gttgaccaga ttcgcaaatc
     2521 cgtccctatc catagctgac actgaaccag agacaacctc ctctataact cgtgacttag
     2581 gaatatcata gccagccttc ttaatggctt gaatctggtt ctctgtggtg gaagtctcta
     2641 ctgatacacg gcagtaagca aaggttcgtg acatagatat ccctctgggt agtcattaaa
     2701 cggttagttt gtcttgtcta tagacattaa agcaaatggt aggtttgttg tctatagcta
     2761 tagtgactct tctatgtaga tattaaacgg acgtttcttg tcgtgggtca catagatact
     2821 tttagggctt ctgtcaagtg atattcgtag attttgcaag ggggaggtgt aaggctgaaa
     2881 cagggctact aagaacacat acatctaagg ctggaaagta cctgagcaga tattcttc
//
LOCUS       DAESHI010000241         2919 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.244, whole genome shotgun sequence.
ACCESSION   DAESHI010000241 DAESHI010000000
VERSION     DAESHI010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.244"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..495
                     /locus_tag="KW011_004373"
     CDS             <1..495
                     /locus_tag="KW011_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBH7662530.1"
                     /translation="GADPDYVIWAKEVQGINRAWTFRHWKGTGTVGVMVATNDPERPA
                     PDESVINAVRDHILPLAPVAGSGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNAL
                     MLRDGVPEGRMYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQAYDP
                     ARSK"
     gene            495..1085
                     /locus_tag="KW011_004374"
     CDS             495..1085
                     /locus_tag="KW011_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBH7662531.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1072..2112
                     /locus_tag="KW011_004375"
     CDS             1072..2112
                     /locus_tag="KW011_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH7662532.1"
                     /translation="MHRIDTLTAVKDKFGPGKNGFTDGNLRTGRLATWLNSAMWNAIQ
                     EEICGVIEKAGIELNKEEHDQLYKAILLLVGGAINEEALLIKNNLSDVEDRDEAVENL
                     GLKPTVDKAKNAVQRDGDTMTGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEG
                     QGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGDN
                     DTGFKQNGDGLLDVYANSVHVLRFQSGSIQSNKAVNVTGRVTPSDYGNFDARYQQRNG
                     GVQDVRYGYEMYYTPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYRPLQK
                     LINGTWYNVASI"
     gene            2115..2660
                     /locus_tag="KW011_004376"
                     /pseudo
     CDS             2115..2660
                     /locus_tag="KW011_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            2684..>2919
                     /locus_tag="KW011_004377"
     CDS             2684..>2919
                     /locus_tag="KW011_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HBH7662533.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVY"
BASE COUNT          758 a          636 c          833 g          692 t
ORIGIN      
        1 ggcgcagacc ctgattatgt tatctgggcg aaagaagttc agggtattaa ccgtgcatgg
       61 actttcagac actggaaagg cactggaacg gttggcgtga tggtggcgac aaacgatccg
      121 gaacgcccgg ccccggatga aagcgtgatt aacgcagtca gggaccacat ccttcctctg
      181 gcacctgttg ccggaagcgg attgtatgta ttcggtgcca cagaaaaagt catcccgatg
      241 acgattgcat tatcgaaaga cacaccgcag atcaggactg caataaaagc agagctgaat
      301 gcactgatgt tacgggatgg tgtgccagag gggcgtatgt atctgtccag aatcagtgag
      361 gccatcagtt tgtctgctgg tgaagtggcc caccggttaa tcgtcccttc atcggatatt
      421 gacctggggg agaccgagct tccggtgctc ggcgagatca cctggcaggc ttatgacccg
      481 gcaaggagta aataatggat tcgttacagg atgattatac aaagctgctg tacggactga
      541 tgccgccggg gcctgcatgg tcagataccg acggtgtact tgacggtctg gcaccatcgc
      601 ttgtgcgtgt tcaccagcgg gctgatgaac tggtgattga aatcgatccc ggtcagtcca
      661 cagagctgat tgaacgttat gaagaattgt atggtttacc tgattcctgt tcccctgtcg
      721 gaacccagac attacgccag cgtcagcaac gtcttgaagc aaaagccaat gtggctggtg
      781 gcataaatga gcagttcttc ctggatcagc ttgaggcgct gggatatacc ggcgtaacga
      841 tcgaacagtt ccagcacttg gatgcaagcc ccgatccgga atggggagat cgctggcgtt
      901 atttctggcg tgtgacgttg ccgatggatg ccggtgctca gtggcagaca tgcacggacg
      961 cctgcaacac accgatccgg acgtgggggg atacggttgc ggagtgcgtg attaataaat
     1021 tatgtccgtc acacaccgtc gttttatttt cctatccaga tgaggatgaa gatgcacagg
     1081 attgatacgc tgactgcggt aaaagataag tttggtccgg gtaagaacgg atttactgac
     1141 ggaaatcttc gcacaggacg tcttgctacc tggctgaaca gtgccatgtg gaatgccatt
     1201 caggaggaaa tctgtggtgt cattgagaag gccgggatag aactgaataa agaagaacac
     1261 gaccagctat ataaggctat attattgctg gtgggcggtg caattaacga agaggcattg
     1321 ctgataaaaa ataacctttc ggatgtggaa gacagggatg aggctgttga aaacctcgga
     1381 ttaaaaccca cggtggataa ggcaaaaaat gccgttcagc gtgatggcga caccatgacc
     1441 ggagagctga aaatccgtgg tgttaatgcg ctgaggattt tcaacgaagc ttttggcctg
     1501 atttttcgtc gttcggaaga gtgcctgcac cttattccca ccagtgaagg tcagggggaa
     1561 aatggcgata ttggtcccct gcgcccgttc accattaatc tgcggacggg tgaaatatcc
     1621 atgtcgcata aagtgtctgt tggcggtggt tcgcaggtca atggtgcgct gggtatcggc
     1681 gttcagaacg ccctgggggg gaattcaatt gttcttggtg ataatgacac cggattcaaa
     1741 cagaatggtg atggcctgct ggatgtttat gccaatagcg tgcatgtgtt gcgttttcag
     1801 agtggcagta tccagagtaa taaagctgta aacgttacag gacgggtaac accgtcagac
     1861 tacggaaact ttgatgcccg ttaccagcag cgaaatggtg gcgtgcagga tgtgcggtat
     1921 ggttatgaaa tgtattacac ccccggcagt aacaccgttt cgtggacatt tcgctcacct
     1981 tcgggacacg ggctgtcagg gatatcgata tcggataccg gccgtaactc agcggataac
     2041 gtcaacggcg tgtattaccg accgctgcaa aaactgatta atggcacctg gtataacgta
     2101 gcgagtattt aacaatgttg catttaaaaa atattacggc aggtaatccg aaaaccgcgg
     2161 aataatatca gctgacaaaa cagtatgatg tcacctggct tttttcggaa gacggcaaaa
     2221 actggtatga agaacagaag aactttgcca gtgacaccat aaaaatggtt tacaccggag
     2281 acgggcgcgt ggtgtgggtc ggtaaggatg tgacaggcat tgaaccccgt aacgccagtg
     2341 ttattgaagt tcctgatatt accgccaacc gccgcattac cgcgccgggt tactggtttt
     2401 accgcaatga tgaatttgtc tttgactaca gactcaaagc ggaagatgag cgtgatgccc
     2461 ttctggctca ggtcagtgcc cggacagggg aatgggaaga agacctgctg ctggggctaa
     2521 tcagcgacga agaccgggaa aaactgaaag cgtaccgtat ttacgcgaaa tcgctgcagg
     2581 cgatggattt cagcaccatc actgataaat cctcatataa cgccattgaa tggtccgtct
     2641 ctccggaagg ttcttcctga tttaatttat cgcgagaaaa actatgtctg tagtgatatc
     2701 aggtgcgctg actgatggcg caggcatccc catgtccgga tgccagatta ttctgaaatc
     2761 ccgtgtaaac acctcagaag tggtgatgcg taccagggct gatgtagtga ccggaaataa
     2821 cggtgaatat tcgtttgagg cacaggtcgg aaaatattgc gtgtatctga aacgggactg
     2881 gcgcgacgag tactgtgttg gcgacattgc tgtatacga
//
LOCUS       DAESHI010000242         2902 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.245, whole genome shotgun sequence.
ACCESSION   DAESHI010000242 DAESHI010000000
VERSION     DAESHI010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.245"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..978)
                     /gene="curA"
                     /locus_tag="KW011_004378"
     CDS             complement(<1..978)
                     /gene="curA"
                     /locus_tag="KW011_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HBH7662534.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLEGNDSNLL"
     gene            1159..1677
                     /gene="mddA"
                     /locus_tag="KW011_004379"
     CDS             1159..1677
                     /gene="mddA"
                     /locus_tag="KW011_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HBH7662535.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1674..2123
                     /locus_tag="KW011_004380"
     CDS             1674..2123
                     /locus_tag="KW011_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBH7662536.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2124..2357)
                     /locus_tag="KW011_004381"
     CDS             complement(2124..2357)
                     /locus_tag="KW011_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HBH7662537.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2443..2616)
                     /gene="ortT"
                     /locus_tag="KW011_004382"
     CDS             complement(2443..2616)
                     /gene="ortT"
                     /locus_tag="KW011_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HBH7662538.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2811..>2902
                     /gene="yncL"
                     /locus_tag="KW011_004383"
     CDS             2811..>2902
                     /gene="yncL"
                     /locus_tag="KW011_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HBH7662539.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWF"
BASE COUNT          698 a          744 c          696 g          764 t
ORIGIN      
        1 caataagttg gagtcattac cctctaaacc atcagtaata tcttcgtggt agtggatttt
       61 atcctctttc acccattgcc ccatctcctt ctgaaactca tggatgcggt gaccataatc
      121 ctgagcgata ataaaccctt gcaagcgaat acgttttttc agcaccgttg ccatcaacag
      181 gggtaaacga tccggaccgg gtggtagctc tgtagcgtta tagctgctca ctaagccaca
      241 aacgggaatg cgcgcagatg tattaagtaa tggtagcact gcatcaaata ccttaccgcc
      301 cacgttttca taatagatat caataccttt tgggcacgct ttcaccagtt gttcggcaaa
      361 atcatccgcg tggtgatcaa gacaaacatc gaagcccagc acctcgatag catggcggca
      421 tttttccgcg ccaccggcaa cccccaccac cctgcaacct ttaagtttgc cgatttgccc
      481 caccgttgct cccacaggac ctgtcgccgc agctaccacc aacgtttcgc cctctttagg
      541 ctgaccgata tccagcagcc ccatataagc ggtaaagcct ggcatcccca gcacacccag
      601 cgaccacgat ggattttgcg gatgatcgcc aagtttcacc agatcatcac cactggatat
      661 gtcatagtcc tgccatccac tgtagcccag cacccagtcg ccaggctgat aatcaggatg
      721 attcgactcc acaacacggc tcaccgtgcc gccgaccatc acgccgccaa tatcaacagg
      781 cggtgaataa gatggctcat cgctcatacg tccacgcata tacgggtcca gggacaaata
      841 aactgtgcgc agtaacacct gtccttcacc cggtgtggcg acatcatctt cttcaagacg
      901 gaaattctcc ggaaccggtg cgccatgtgg acgcgaggcc agaacccaac gtcgattacg
      961 ctgcttttgt tgccccataa tgctctcctg tgcttttggc atgaatccat caatagtagc
     1021 aggctcacgg gattggtttt gtttgcagcc gtcacatccc ctctgcaaca cgcaataatt
     1081 tcatcgagga atccgttgct tcgggctgtt ttcgatagta ttgataacat catcatcatt
     1141 attttatcga ggtcagacat gtccatccgt tttgcccgca aagccgattg tgctgccatt
     1201 gcggaaattt ataaccacgc cgtgttgtat acggcggcta tctggaatga ccaaacggtt
     1261 gatgcagata accgcattgc ctggtttgaa gcgcggacca tagcaggtta tccggtgctg
     1321 gtaagtgaag aagatggcgt agtgacggga tatgcctcgt ttggcgactg gcgtagtttc
     1381 gatggttttc gccataccgt ggaacattcg gtttatgtcc atcccgatca tcagggcaaa
     1441 ggtctggggc gtaaattgtt aagccgattg attgatgaag cgcgggattg cgggaagcat
     1501 gtcatggtcg ccgggatcga atcgcaaaat caggcctcgc tgcatctgca ccattcgctg
     1561 ggatttgtcg tcaccgcaca aatgccgcag gtgggcacta aatttgggcg ctggctggat
     1621 ctgacattta tgcagttgca actcgacgaa cgcactgaac cggacgcgat tggatgaatc
     1681 agtcgctcac ccttgctttt ttgattgccg ccggaattgg tctggtagtc cagaacacgc
     1741 tgatggtgcg catcacccag acctcctcta ccattctcat cgccatgttg ctgaactcac
     1801 tggtggggat tgtgctcttt gtttctattt tatggtttaa acaaggcatg gcggggtttg
     1861 gcgaactggt gtccagcgtg cgctggtgga cacttatacc cggcttgctg ggatcgtttt
     1921 ttgtcttcgc cagtatcagc ggatatcaga atgtgggagc ggcgacaact attgcagtac
     1981 tggtcgccag ccagctaatt ggcggtctga tgctggatat cttccgcagt catggcgtac
     2041 cactacgggc gttgttcggc ccaatttgtg gcgcaatttt acttgtggtt ggcgcctggt
     2101 tggtagccag acgctcattt taatcagagg atggtgccgc ctttggttaa ctgttcgtgg
     2161 cgcgcttcca tatcttcttt atgcttgcga ccgtgatgag caatagcggt gcgcaaacgc
     2221 tgctgctggg tgtagcgatc ttcccgactt aactccgcgt catcgctcag ggcgattaat
     2281 aattcgttca tatgggcaat gacgctctct tcaatcgcgg catccacgcg cgcgatgact
     2341 tcatccagat gcgacatact ttctccttaa ccacttgtga aatttatcga ttcatccaaa
     2401 tgatataagt ctgcgtggtg ccgtcaagcc agtgagtaag atttaaaaca gtgaaaacaa
     2461 cagcgccacc gggaaactca tcggccatgt tgcccccacc agaaaggcgc ttaagaaacg
     2521 gatgcgtttc ttatcgtgag aaagaaacca ggtgataaga aatgcgattg ctgccataac
     2581 cgcataaaaa acaagcatat gttgatagag agacatgtac gggcgtccag ccagatgaat
     2641 aaaacgcgcg caaatatgcg tcattttttg acgtgcatca agttttttca ccagtttgag
     2701 aaaaaatcat caaacagcaa cttgccctta tccatcttca gaatgagtat tattgcggat
     2761 tttcttaacc cgtactatac acataggggc aatgataaaa ggtggcaaaa atgaatgttt
     2821 ccagtagaac tgtagtactg ataaatgtct ttgctgctgt tggtttgttt actcttatct
     2881 ctatgagatt tggctggttt at
//
LOCUS       DAESHI010000243         2900 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.246, whole genome shotgun sequence.
ACCESSION   DAESHI010000243 DAESHI010000000
VERSION     DAESHI010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.246"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..1199)
                     /locus_tag="KW011_004384"
     CDS             complement(<1..1199)
                     /locus_tag="KW011_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HBH7662540.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
     gene            1492..1586
                     /locus_tag="KW011_004385"
     tRNA            1492..1586
                     /locus_tag="KW011_004385"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1526..1528,aa:Sec,seq:tca)
     gene            1886..>2900
                     /locus_tag="KW011_004386"
     CDS             1886..>2900
                     /locus_tag="KW011_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBH7662541.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMST"
BASE COUNT          845 a          691 c          665 g          699 t
ORIGIN      
        1 gcgctgttct gcgctttttc tgccgcatca tatccgccat aagccagcat ccagccgata
       61 agagcgccgc cgaaggccaa ccccagtttg agcacaaaca gcgtgccagc aaaactgatc
      121 ccggtcaggc gtttaccatt gcaccactcg ccgtagtcga cggtatcgga catcattacc
      181 cactggatag gtgtcaccag ttgatgcaac acaccaatca cgaagatgaa gacaaacata
      241 gtgatgctgg cctgcatggg aacaaagaac atcgcgaggc taatcactgc cagcagggcg
      301 ttcgtccacc agaagatagt gactttacat ttccagtcgg tcagaggttt tgccagtgcg
      361 gaaccaatca ggttacccac gcaataagtg gtgagaaaag cgacaaacac ttccggcgtg
      421 cccaaaatcc atgtgacgta atacatcatc gccccaccgc gtacgcacac cgccaggata
      481 ttgaaaatgg ttagtaaacc gacaatccgc cactggtcgt tttgccagat atcacgtaaa
      541 tcttcccgca tagacgttgt tgtaggtggt gcttcaacgc gttctttagt ggtgaagaaa
      601 caaaatgcca gcatcatgaa tgccaccacg gaaaggaccg cgataccgcc ctggaaaccg
      661 agtggtttat tatcaccgcc aattaaatta accagtggca tcatcagaac agtagaaagc
      721 atgcctcccg cggtcgccag cacaaaacgc caggattgca gcgagatacg ctgagtcggg
      781 tcattggtga ttacaccacc caatgcgcag taagggatat tgacgacggt ataaagtaag
      841 gtaagtaggg tgtaagtaat tgctgcatag atcattttgc cgttcatact gagatctggc
      901 gtgctatagg ccagtacaca gacgatcccg aatggcagtg cgccaaacag tacccacgga
      961 cgaaatttac cccagcgaga gcgcgttcga tcggccaaca accccatgca aggatcggaa
     1021 atcgcatcca gtgcacgagc gaccaaaaac atggttccga caaatccggc aggaatgcca
     1081 aaaatatcgg tataaaagaa catcatatat aacattacgt tatcgaaaat aatgtggctg
     1141 gcggcgtctc ccatgccata accaattttc tctttaacgg acaacacttc actcttcatc
     1201 tgcttttcct tgatgctata ggggtgctga gaccgataac gcattttgta aaccatccct
     1261 gtgagagcga atattccgat atctggttat taatttatga ttcttgtttt atgtgatcgt
     1321 ggtagcgtta attccgctca tatatcattg taaaatatgg gttttatatg agttataatg
     1381 ctttcgtgat aatacgctgc gtgtattagg cggaaaaaac tgatctgggg gatgtagaaa
     1441 ctcaaggaag tagctataat gcgccccgcc tccatgtagc aatcgaggcg cggaagatcg
     1501 tcgtctccgg tgaggcggct ggacttcaaa tccagttggg gccgccagcg gtcccgggca
     1561 ggttcgactc ctgtgatctt ccgccagttt acctcttctg aactctccca atgtcaataa
     1621 atctcttcta atcccttgta tatcagtatt tatttgtatc ctgaggtcaa cctacttcac
     1681 cccatatcaa gtttcttttg ggggtacttt agggggtgtt atttcctatt gaaatacaag
     1741 cacccccata ataggaaaaa tgtgatacat aacaacggct tttaacaata tctttgttat
     1801 acaaggggtg tattgggggt atcaggttag ttactggcag aaatccccct caggaacatg
     1861 aattcatcaa aagccggagg atgtcatggc attaaccgat atcaaagtga aaaccgcaaa
     1921 acccaaagac aaaccctata agcttgctga tggcggcggt atgtatctac tgattaacac
     1981 taatggttct aaatactggc gcatgaagta ccgctttgct ggtaaagaaa agatgctttc
     2041 tattggcgta taccccgatg taacgctagc cgatgctcgc gagaaacgca gtgaagccag
     2101 gaaactcctc gctgctggag gcgatccagg ggaggcaaaa aaggaagaaa aaatcgcgca
     2161 gcaaatgagt ctgaaaaata cgttcgaggc tattgctcgc gaatggcacc agtcaaaagc
     2221 tgatcggtgg tcattacgct atcgtgatga aatcattgat acctttgaaa aagacatttt
     2281 tccctacatt ggcaagcgcc ctattgccga aatcaaacca atggagttac tggaagcact
     2341 gcgtaaaatg gaaaaacgtg gagctctgga gaaaatgcgc aaggtccgcc agcgctgtgg
     2401 tgaggtgttc cgctatgcaa ttgttactgg ccgggcagac tataacccag cccccgatct
     2461 tgccagtgct ttagctacac cgaaaaaagt acattttccc tttcttactg ctaatgaact
     2521 tccccacttc ctcaacgatc tggcgggtta taccggaagt ataatcacta agacagctac
     2581 tcagatcatt atgttaaccg gcgtacgaac acaagaactg cgttttgcac gctgggaaga
     2641 tatcgatttt gagacaaaat tgtgggagat cccggcagaa gtaatgaaga tgaagcgacc
     2701 tcatattgtg ccgctctctg agcaggtcat tatgctattt aagcagcttg aaccaatctc
     2761 aaaacatcat cccctagtct ttatcggtag aaatgatccc cgcaaaccaa ttagtaagga
     2821 aagcattaac caagtaattg aattacttgg atataaggga agactcacag ggcacggttt
     2881 cagacacacc atgagtacaa
//
LOCUS       DAESHI010000244         2890 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.247, whole genome shotgun sequence.
ACCESSION   DAESHI010000244 DAESHI010000000
VERSION     DAESHI010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.247"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            1..905
                     /locus_tag="KW011_004387"
                     /pseudo
     CDS             1..905
                     /locus_tag="KW011_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533802.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
     gene            908..1441
                     /locus_tag="KW011_004388"
     CDS             908..1441
                     /locus_tag="KW011_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBH7662542.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1470..1997)
                     /locus_tag="KW011_004389"
     CDS             complement(1470..1997)
                     /locus_tag="KW011_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBH7662543.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(1999..2841)
                     /locus_tag="KW011_004390"
     CDS             complement(1999..2841)
                     /locus_tag="KW011_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH7662544.1"
                     /translation="MTGELKIGTVNALRIFNDTFGLIFRRSEDFLHFIPTAEGQGENG
                     NIGPLRPFAINLRTGAISVSHGAKIKGGLAIGATDNALGENSIVLGDNDTGFRQDGDG
                     IISFYSNGSRIGHIDELGLHLYKDIESNGSNFRLKSNYRHHITFANEDGRIRMFLWKD
                     NGGDGVHINNGSDGGGDFIFKTDGGFALGSGAQVASSGDIYGSVWENNWLSTWLHNHV
                     VRDIRLGSIEYKNVWRDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYN
                     VGSV"
BASE COUNT          812 a          637 c          621 g          820 t
ORIGIN      
        1 gcacaagcgg ggagaaaata ccgttactca gtacagcgaa tacctggact aatcgacaaa
       61 cattcagcgg tggcctttct ggtgaactat ccggcaatgc ttctacagct gcaaaattaa
      121 aaactgccag gaaaataagc aatgtggctt ttgatggttc ctccgatatc acattaaaag
      181 caagtcatgt tggtgcgttt gccttaggga aaacaggaag caccgttgcg aatgataaag
      241 cagttggatg gaactggagt agcggagcct ataacgcaac tattagtggt gcatcaacgt
      301 taattattca tttttatatg ggagaaggaa gttgtcctgc agctcagttt cggattaatt
      361 ataaaaatgg cggtattttt tatcgttcag cccgtgatgg ttatggtttt gaagccgact
      421 ggtccgaatt ttacaccacc agaaaacctt cagcaggaga tgttggtgca ctgccgttat
      481 ctggtggtca actgaatggt gcactgggta tcggaacatc cagtgctctt ggcggtaatt
      541 cgattgtatt gggtgataat gacacgggct ttaaacaaaa aggcgatggt aatctggatg
      601 tttatgctaa taacgtccat gttatgcgct ttgtctccgg aagcattcaa agtaataaaa
      661 ccataaatat tacgggacgt gttaatccct cggattacgg taactttgat tcccgctatg
      721 tgagagatgt cagacttggc acacgagttg tttagaccat gaagaaaggc gtgatgtatg
      781 aaaaatcagg ccatgtaatc acggggcttg gtattatcgg tgaagtcgat ggtgatgatc
      841 cggcagtatt cagaccaata caaaagttaa ttaacggaac atggtataac gtatcgcagg
      901 tataatcatg cagcatttaa aaaacattgt cgcaggtaat ccaaaaaccg ttgaacaata
      961 ccagctaaca aagaattttg atgttatctg gttatggtcc gaagatggaa aaaattggca
     1021 tgaggaatta aaaaactttc aggaggatac aataaaactg gcttacacag ttgagggaat
     1081 aattattgct atagacaagg atgtatcagc aattaatcca gaaggtttaa gtgtcgttga
     1141 gttgcctgat attacagcaa atcgccgggc tgatatttcg ggcaactgga tgttcaaaga
     1201 tggtgcggtg ataaagcgaa cttataccga ggaagagcaa aggcaacaag cggaaaatga
     1261 aaagcagagc ctgctgcagc tcgtcaggga taaaacccag ctgtgggact ctcagctaca
     1321 gctgggcatc atttccgatg agaataaaca aaaattaacc gagtggatgc tctatgcgca
     1381 gaaggttgaa tccacagaca cctccagcct gccagtaacg tttcccgaac aaccagaatg
     1441 agagaaggcc cgctatcggg ccttaatttt tattcaggct tttgtggcca ttcaggattt
     1501 gccgtatcca cacggctgac cagaacactg tagcgttccc atgcttccag tcgtgtgcgt
     1561 tcctcgtctg ttgccatatt cagcctgaca gcgcgttcca gcggctggat gactgattca
     1621 gcttcggaaa gcaatgcggc cttttgtgat tcggcctgtt gttgcagttc ctgttctgta
     1681 tattccctct ttactacttt accatccaga aacatccatt ttccgttatt gtctgcgtga
     1741 cgatttgccg taatatcagg aacttcaact atgcttagcc cttcaggatt aatagttgaa
     1801 acgtctttac tgacagcaac gatgatgttg ttctcatcgt aagcaatttt tatggtgtct
     1861 ggctgaaaat tcttttgttc ctcataccag tttttgccat cttcgcaaaa gagccatacc
     1921 acgcccgctt ttttagttag ctggtattgt tccggtgttt tcggattccc ggccttaata
     1981 tttttcaggt gcatcatctt aaacactccc cacgttgtac catattcccc caatcaattt
     2041 ctgaattggc cttctggcaa cagtgtcgac aatatcatcc gcattgctgt taattgcggc
     2101 tgttaaaaca taacctgacg catcgccaaa gccgtagtcg cgccatacgt ttttatattc
     2161 aatgctgcca agacgaatat cccgaacgac atgattatgc agccatgtgc ttaaccagtt
     2221 gttttcccac actgaaccat aaatatcgcc actagacgca acttgcgcac cacttcccag
     2281 cgcaaatccc ccatctgttt taaaaatgaa atcaccacca ccatctgaac cgttattaat
     2341 atgaacacca tcaccaccgt tatctttcca caaaaacata cgaattcttc cgtcttcgtt
     2401 ggcgaatgta atgtggtggc ggtaattact ttttaatcta aaattgctgc cattagattc
     2461 aatatcttta taaagatgta atcctaactc atcaatatgt ccgatgcgcg aaccatttga
     2521 atagaagcta ataataccat ctccatcttg cctaaatccg gtgtcgttat ctcccagaac
     2581 aatggagttt tcacccagtg cgttatcagt agctccaata gctagacctc ctttaatttt
     2641 ggccccgtgg ctgacagata tagcacctgt tctcagattt atagcgaatg gccttaatgg
     2701 gccgatatta ccgttttcgc cttgtccttc agccgtcgga atgaaatgaa gaaaatcttc
     2761 tgaacgacgg aaaattagac cgaaggtatc attgaaaatt cgcagcgcat tcaccgtgcc
     2821 aattttcagt tctccggtca ttttatcgcc ggaacgctga acggcgttac cagccttgtt
     2881 taccgtttcc
//
LOCUS       DAESHI010000245         2797 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.248, whole genome shotgun sequence.
ACCESSION   DAESHI010000245 DAESHI010000000
VERSION     DAESHI010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.248"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            1..617
                     /locus_tag="KW011_004391"
                     /pseudo
     CDS             1..617
                     /locus_tag="KW011_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
     gene            614..1093
                     /locus_tag="KW011_004392"
     CDS             614..1093
                     /locus_tag="KW011_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HBH7662545.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(1147..2097)
                     /gene="corA"
                     /locus_tag="KW011_004393"
     CDS             complement(1147..2097)
                     /gene="corA"
                     /locus_tag="KW011_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HBH7662546.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            2467..>2797
                     /locus_tag="KW011_004394"
     CDS             2467..>2797
                     /locus_tag="KW011_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662547.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          842 a          620 c          591 g          744 t
ORIGIN      
        1 tccggaacaa aaatatcaca acgtgaatgc actgatattt tatttgcaga aatgaaagaa
       61 ctagcaaaaa tgttttcctt ttttggacaa tataaaacac ttatcgaaga tctgattgaa
      121 cattttagat atggaaatgg cagcaatttt catagccaac aattaaattt atcttttcat
      181 gaaaaaataa atatggttat aatagtccaa tacgaataat caaagaatgt atcgaaaatg
      241 gcattaattc aactccctcg acaggatacc aacctcttat cctgcaaagc attaaaacca
      301 aattactttc atcaagacta aataaattta atgacttcga ggacagtttt aatggcttag
      361 gaatcagtgt tcatgatatt tcagctcaaa aaatatctct tttaagtttt caaaaatacg
      421 ctataggatg gagtgcaaca attcattttg tagcacagga ccacttcggt cttgatgtaa
      481 ctgatatcaa aaacaagact tacagcaaat atagattttt tcgcatatgg ttctttttac
      541 aacggcataa agactttgct ttcaaacctt ttttcaccaa ttttaacact atcgaaagaa
      601 tcgaaaatta tctatgaaaa gcaaactcca gaaaattata ttatgtttat ttcttctatg
      661 ctgcatctat aatttatgga cattgcgtcc tgtacagatt ttgtatacat actctgatgc
      721 tggcaattca gtctttctgg tcgttgatca tctgccatgg actgacagcg ataaaataaa
      781 ttggtatctg aagcatcaga atgaaattaa aaatcaacat cccctaccag aaggtagttg
      841 gcatacctgg tatgtcattg atatcggtaa tggcttcact gactataaaa aatatatcga
      901 agggccgtat gaagatctct actgtttccc gacaattaag agcaatgata attgcatcgt
      961 gaaaaattac ttaatggtta ttaatgaata cccttatcgt aacactcata ttggtattaa
     1021 cgattttact gagtatcaat taacacaaga aaataaaata gagcgagttt tcaatccgca
     1081 tgactttaaa taagacaatt tttagaaata agccggataa aaaaatagct ttatccggca
     1141 ctcatattac agccagttct tgcgcttaaa gtacagatac ggtgccaggc ccgcgaggat
     1201 cataaagata atcgcgcctg ggtagccgaa actccacttc agttctggca taaactcaaa
     1261 gttcatccca tagctggaag caacgagcgt cggcggcagg aataccacgg ataccaccga
     1321 gaagattttg atgatgcggt tctgctcgat gttgataaaa cccatcgccg cctgcatcag
     1381 gaagttcacc ttctggaaca gggattcgtt atgcggcagc agggattcga tatctcgcag
     1441 gatttcacgc gcctgctcca gttgcccacc cggtaaacgc gctttacgca ccaggaagtt
     1501 gagcgcgcgc tgggtatcca tcagacacag gcgaactttc cagccgatat cttccagttc
     1561 cgccagagtg gagagcgcct cgtcgtactc atcgccctga tgcccttcca taatcacccg
     1621 gctcaactgc tccaggtcgc tatagatatt ttcaatttca tctgccaact gttcgatttt
     1681 ggtttcgaac agatccagca gcagctcgta ggcgttaccg tctaccatcg actggctacg
     1741 ggcacgcata cgatacagac gaaaagcggg cagttcacgc tcacgcagag taaacagacg
     1801 accatcacgg atggtaaatg ccacagtgga gttaccggcg tgatcttccg catcttcaaa
     1861 gaagaagaag gagtgaatat gcaggccgtc gtcgtcttca aagaaacgtg ccgatgcttc
     1921 gatgtcttcc agttccgggc gggttgccag gctctggcca agttcagatt gtacgcgcag
     1981 tcgctcgtcg tcgtccggtt cgacaagatc aatccatact gcatttacaa ggggttgtga
     2041 ctcttcgact tccagccggg tcagtcggtt attttccagt tgaaatgcgc tcagcatgac
     2101 cgggactccc aatgcttaaa atatcggaca gttcggtggg cacacagaaa caaattgggt
     2161 ttcagaccat taaacagcct gactcagcgc gacgggaaaa attgaggtcg ctgacaaccg
     2221 ctaaggctat cagcaaaaag ggatagcctt aggagttgat cctggatgac aggatagtga
     2281 gccagtatct actgggtgtg tccaaggcga atgtcctctt agagtgatcg tgcgcgcatg
     2341 ttacgccagc aaaattttgc cgtcaacacg caacagaaca ccacagaaca atagttgaac
     2401 ttcgcgcttt ttggtactcg tcaaggtcgc taacaggata aaaaaatcca acatccagga
     2461 cctataatgg cgcatcggct actcattggt aaaggaatga tcaccttgaa tctcaaacgg
     2521 atttttctcg ccctcacctt acttcccttg tttgctgttg ccgctgatga ttgcgcactc
     2581 tcagatccga tgctgaccgt acaggcgtat accgttaatc ctcaaacaga gcgggtgaaa
     2641 atgtactggc aaaaagccaa tggcgaagcg tggggaactt tacatgctct gctggtggat
     2701 atgaatagtc agggtcaggt gcagatggcg atgaacggcg gcatctatga tgaaagctat
     2761 gcgccgctcg gtttgtacat cgaaaacggt cagcaga
//
LOCUS       DAESHI010000246         2729 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.249, whole genome shotgun sequence.
ACCESSION   DAESHI010000246 DAESHI010000000
VERSION     DAESHI010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.249"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(423..680)
                     /locus_tag="KW011_004395"
     CDS             complement(423..680)
                     /locus_tag="KW011_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662548.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(677..1174)
                     /locus_tag="KW011_004396"
     CDS             complement(677..1174)
                     /locus_tag="KW011_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HBH7662549.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1376..1834)
                     /locus_tag="KW011_004397"
     CDS             complement(1376..1834)
                     /locus_tag="KW011_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBH7662550.1"
                     /translation="MSRVTAIISALVICIIVCLSLAVNHYRDNAIAYKEQRDNKASEL
                     EKANATIADMRKRQRDVAELDARYIKELADANATIESLRADVSAGRKRLQVAATCAKS
                     TTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1831..2328)
                     /locus_tag="KW011_004398"
     CDS             complement(1831..2328)
                     /locus_tag="KW011_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBH7662551.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKD
                     IVGVWTVCYGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRCAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGNQWKGLMTRREIEREVC
                     LWGKQ"
     gene            complement(2328..2543)
                     /gene="essD"
                     /locus_tag="KW011_004399"
     CDS             complement(2328..2543)
                     /gene="essD"
                     /locus_tag="KW011_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HBH7662552.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          649 a          708 c          621 g          751 t
ORIGIN      
        1 atacagcgca tgatgtgtca ggcttgaata cctttatcct ttaaaaggga tatcagttaa
       61 gttatcccgt gtagggtata agccattatc aaagccactc tgtagggaat ggcttttgtg
      121 atggcatcac ttactcttta cgctgctatc ccactcatcc cggaattttg atgggttatt
      181 gaaaccttct gctgacataa caactccttc aatgtttggc tgaaattggg atgtctttcc
      241 atcagtccgc caccacaaag aatctttttt gccataaggc aggaggttca tctttcagtg
      301 gctgccggtg ttatttcccc acttactggc ttgggttgtt tcgtggtact gccgttaact
      361 ggtggcccag aataaattcc ggtttcatta tcaagcccac ccgtaaatgg gctttgtaat
      421 ggctacttcg cttttgcttc cgctcgctta cgccggcgct cttctttcct ctcggctttt
      481 gccatgtcca tgaatgcctg catgatcgag ttccgcatca tgtagctaac aaagtgatga
      541 ttgacacagc cgttgaggcg cagctgctcg ccaaactcat ccaccgaggc caatgcttcc
      601 atcatgccct tctcgccttt catgaactct gagaagtcgc gccccgctct ggaggcgcat
      661 tcaataacat gatcactcat cccggaagcc cggggatcgt aatctgcagc tggttagcca
      721 gggagttaat ctcagcgacc aacactggct tcgtatagcg ccatgctgcc agcccttgtc
      781 cgcagaagct cgccatgtct ttcttctggt caaattcatg acatttcatg ttgagctgcg
      841 cacttaagct gttgcgatgc tgaagttctc cggtgaagta gtcatcgagg actttatagg
      901 ccgcgtactt gaacccgggg tttaaccaag ccgcataatc gtaagcaaca aacttcccgc
      961 catatgttcc accgtgtaca ccgcgctcag taaaaaccac agattcgtgg tttttctcca
     1021 gctcggctaa gaactctttg gtctgcttgt ttcgcaggta gtggtaaggc gattcagatt
     1081 cacttttacc actggctttc cacatatcag tgaggcagat catgccatct tcaccgatac
     1141 gaattggttg attgaagagg gttaatgatt tcatagcgtg tacctactct ttgaaatgaa
     1201 cctttgccgc acaggaaacc agcccaccga ggctcgccag cactaactgg tatcctcaaa
     1261 ggcccattcc aaaggggcag gttcggtgta aaaaacatgc gttgcggtac gcatttattg
     1321 caaaaagccc cgcatcgcga ggctcattaa atggactttg tgatttgcaa aaaaattatt
     1381 tcaggcattg cgtcctgatg tattcctgca ggtagttaac ctgcgcggtt atcttgtcga
     1441 ttccacttcg gagacggtaa taattgagtt cagcatctgc tgtaagtcct gggctttctc
     1501 catcgcccat gccgctggct ccggtcgttg actttgcaca ggtggcggcg acttgcaggc
     1561 gcttacgccc agcagaaaca tcagcacgga gactttcgat agtcgcgtta gcatcagcaa
     1621 gctcctttat gtatcttgcg tcgagttctg ctacatcacg ttgacgcttc cgcatgtcag
     1681 cgatggtggc gttcgccttc tccagttcac tggccttgtt atcgcgctgc tctttgtagg
     1741 cgattgcgtt atcacggtaa tgattaacag ccaatgacag gcagacgatg atgcagataa
     1801 ccagagcgga gataatcgcg gttactctgc tcattgtttc ccccacaaac agacttcacg
     1861 ctcaatctca cggcgagtca tcagtccttt ccattgatta ccgccagcgt atgtccagcg
     1921 ccgtagctga tcacatgcgc ctttaatatc accctggttt attttgcgaa gaagcgtcga
     1981 tgttctgaaa ttgccagcac ccacgttgta gacgaacgag taaagagcgc agcgcgttgt
     2041 ttccggtata tcgactttga tgtacgggtt aatttgtctg gcgacagtgg caaggtcttt
     2101 attcaggagg gctttgcact ctgcttcggt atacgtttta ccgggaatga tgtcttttcc
     2161 tgtatgcccg taacatacag tccatacacc aactatgtct ttgtaaggat tatgtctcac
     2221 accttccaga ccatcgttac cacctgggcc agtgattaac acagatgcta tagcaatagc
     2281 cccgccacca atagcagcag caacagcttt tcgtaatgat ggaggcatta tccacctctc
     2341 gcagccttgc gcttatcttc tttaatcttg aaataaaggt ttgtcaggta cgtcagcagg
     2401 ccaaatacca ggctacccag cactccaatt gccgcccact gtgagggcgt gactttatct
     2461 agcagctgta aaaaccagta cccggcacta cctgctgagg tgccataggc gacacccgtt
     2521 gttaacttat ccatggattt cataacccca cctcgcagac aaagcgggtg taaattgagg
     2581 gaatactacg aaacgtaaca gactcggagt cagtgaataa ctcaggtatt gggttatcag
     2641 ctaatatcga gactcaaaaa atggaaaaac ccgctcgacg gcgggtttaa gctgtgtgac
     2701 gaagtaacca ctcttaacag cataaccaa
//
LOCUS       DAESHI010000247         2717 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.250, whole genome shotgun sequence.
ACCESSION   DAESHI010000247 DAESHI010000000
VERSION     DAESHI010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.250"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..253
                     /locus_tag="KW011_004400"
     CDS             <1..253
                     /locus_tag="KW011_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HBH7662553.1"
                     /translation="AALPVSWIQMLAGLALLSTIGGSLYQALHNERERERERDAAVVA
                     FLVTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNRY"
     gene            complement(293..1501)
                     /locus_tag="KW011_004401"
     CDS             complement(293..1501)
                     /locus_tag="KW011_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH7662554.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMHRQLEYKQLWRGGQVLAVPPAYTSQRC
                     AYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            1509..2010
                     /gene="tnpA"
                     /locus_tag="KW011_004402"
                     /pseudo
     CDS             1509..2010
                     /gene="tnpA"
                     /locus_tag="KW011_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(2028..2696)
                     /locus_tag="KW011_004403"
                     /pseudo
     CDS             complement(2028..2696)
                     /locus_tag="KW011_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
BASE COUNT          628 a          666 c          673 g          750 t
ORIGIN      
        1 ggctgccctg cccgtaagtt ggatccagat gctggcaggt ctggcgctgt tgagtaccat
       61 tggcggcagt ttgtatcagg cgctgcataa tgagcgtgag cgtgagcgtg agcgcgacgc
      121 ggcggtggtg gcatttctgg taacggcaag tggattgacg ctggtcggga ttggttctgc
      181 gttctgggga ttaattgccg gaggcgtttg ttacgtggtg ttgaatttaa tcgctgacag
      241 aaaccgatat tgacatcctc cacgccctga aggacgtgga tccctgctac gttcaggctg
      301 tcgcctgaat catttcggtg ggttcctgct tcaacgggcg gcctgactgc accatctctc
      361 cacaggcaag aacggcgtgc cccgccgcta aaatgttacg agcaccattt acatcggcgt
      421 tcgctgtata tccacatacc tggcatctga atttactttg tgacaggcgg ttctctttcg
      481 ctgtatgacc acagtacgcg caacgctggc ttgtatacgc tggcggaaca gcaagcacct
      541 gaccgccacg ccagagctgc ttgtactcaa gctggcggtg catttcatac cagccctgat
      601 ccagtatcga acggtttaaa cctgattttg cccggacatt gcgccccggc tggctgaccg
      661 tacccgctgc tgactttgac atgttgctga ccttcaaatc ctcaatgact atcattgcgt
      721 ggtttttgct gacggccgtt gtgactttgt gaaggtagtc cctgcggata tttgcgatac
      781 aggaatgcag tcgctgtatt ttgcgtttct gcttctgcca gttgttgctg aatttgacct
      841 tgcggcttaa ctgtcgctga agtctcgcca gcgtcttctg gtttttctga aaactgttta
      901 caggctcaaa gactgtgcca tctgacagcg tggcgagttt agccacgcca gcatccagcc
      961 cgaccattga tgctgaaggg tgagccggag tggatacttc actttctgtc tgaatactga
     1021 tgtaccacgt accgcaggac tggctgacag tgacattttt cacaacaccc gtgacctgac
     1081 ggctattccg gtagcgcatc cagcccagtt tcggcagaaa aatacggctg ttttcctgat
     1141 cgagcttaac gccctgcggg tagcggaatg catcattctg tccccgcttt ttgaatcggg
     1201 gaaaagcagc ccgcttccgg aagaagtttt tgtaagcccg ctcaaggtct ttcagtgact
     1261 gttgcaatgg ctgtgaggga gaatctttaa gccattgcgt ttcagtggca tttttccact
     1321 caaccagcca ggaagccatt ttcccgtaag ggatgtattt attaccggcc tcatgattct
     1381 cattctgacg tgccagagca cgattgaaaa cgaaacgaca tgcgcctgcg aagcgcctca
     1441 tttcacactc ctgttgacca cctggtctta actggaattt aaatgcctgt aatcgtttca
     1501 caggtcaaat gatactttgg tctgtgaaaa aagaaaccga tatccggcgt ggaaggcatt
     1561 gtgttttcct gatgcatgtt cacctggtct ttgtcactag ataccgacgc cagatttttt
     1621 gacgcgacag aaaaactacg cacttacttt tcaaatgtat gtgcttattt tgaagctgaa
     1681 ctggttgaaa tggatggcga accagatcac gtccatttgt taattaacta acctcccaaa
     1741 ctggcgatat ccagtctggt aaacagcctc aaaggcgtat cgggtaggtt actgcgacga
     1801 gatcgaccag atattgcagt caggtattac tacaaaggcg ttttgtggag tcctggctat
     1861 tttgccagta gctgcggagg tgtgccaata tccgtcatcc gccaatacat tgaacaacag
     1921 caaacacctg gtcaggtgga aaaccgcgcc ttatatcccc gccctgaagg atggggtttt
     1981 acggcgcacc ggataaaaag cgccatctaa cgatggcgcg tctggaacta ctttttgtta
     2041 acgtcaaaca tggtggcatc ggtcgccatg tcatcaataa cttgtttaat attttcgaaa
     2101 gccattggcg tactttcgtt attcaggtct ttaccttcgc cctgacgaac gactttgata
     2161 accggtttat tagtcgctgc atcaatcagc tcaccttcaa aatagaggcg agtatccatg
     2221 gtacggtggc ctgtagccat ttgcgtcccc gcaaccacta atgcaacagg aaccacttca
     2281 tagaattgca gcccttcttt gctggtatct acaccggtaa tggccccacg gaaaatcaga
     2341 ctacgcggcc cagcggtggt aaccagtggt ttacgctgcg ctatcgcttc tttcatttcg
     2401 gtgttggtat agttcaaaat tttatccaga actttctgcc ctacctaggt agaaggtttt
     2461 ggcaccggat aataagtgat tgggttccag acgatgctgt catatttgct ttgatcaaaa
     2521 ctcgggtcta cccaacgtaa aacaggttta cctgttgctg aggttgtttc ttttaaatca
     2581 gagtaattgt ttaaaaaacc agaatattta tctggctggg tgattttaga ggcacaacca
     2641 gacagagcca acaagccgca taaagctgca actttcgcaa atgatgtggt acgcatgaat
     2701 agtttcctga gatgggt
//
LOCUS       DAESHI010000248         2651 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.251, whole genome shotgun sequence.
ACCESSION   DAESHI010000248 DAESHI010000000
VERSION     DAESHI010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.251"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(25..315)
                     /locus_tag="KW011_004404"
     CDS             complement(25..315)
                     /locus_tag="KW011_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HBH7662555.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(305..742)
                     /gene="ratA"
                     /locus_tag="KW011_004405"
     CDS             complement(305..742)
                     /gene="ratA"
                     /locus_tag="KW011_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HBH7662556.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            913..1395
                     /gene="smpB"
                     /locus_tag="KW011_004406"
     CDS             913..1395
                     /gene="smpB"
                     /locus_tag="KW011_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBH7662557.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1610..1972
                     /gene="ssrA"
                     /locus_tag="KW011_004407"
     tmRNA           1610..1972
                     /gene="ssrA"
                     /locus_tag="KW011_004407"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2171..>2651)
                     /locus_tag="KW011_004408"
     CDS             complement(2171..>2651)
                     /locus_tag="KW011_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HBH7662558.1"
                     /translation="AVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAR
                     EARRQEIEAWRTEQEAKPFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWGD
                     ADNQQVKLSMPELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLEDLGTIRAFDVT"
BASE COUNT          698 a          718 c          600 g          635 t
ORIGIN      
        1 caatgagcac cttttttctg tctgttattt attcgctgat ttttctgctc gttgcctgcg
       61 aagctctttc ggatcggcaa tgagagggcg ataaatctcc acccgatcgc catcatgcac
      121 actatcgctt agttttgccg gacggctgta aatgccgact ttgtttttag ttaaatcgat
      181 atcggtacgc aattccagca agccactggc gcgaatagct tcttcaaccg tcgcgccctc
      241 ctgcagcgtc acgcgctgca ggtactgctt ctcaggtagc gcataagcca cctcaacggc
      301 aattttacct agcactgtaa acctctttcg cacgaaccgt aaaagcctgg accatattag
      361 acgccagctc tttaaacacg cgaccaaagg cgagttcaat caacttattg gtaaactcaa
      421 agtcgagatg aaactcgata cgacacgcct cctggctcag cggcgtaaac ttccatccac
      481 caatcaattt cttgaacggc ccatccacca gattcataag aatactttgg ttactggtca
      541 actggttgcg ggtagtaaac gttttgctga tcccagcctt agagacatct accgccgcag
      601 tcatctgccc aggagtggac tccagaatcc gacttccggt acaacccggc aaaaactgag
      661 gataagactg aacgtcattc actaactgat acatttgctc cgcgctgtag ggtaccagtg
      721 cggtccggct aatctgaggc ataactattt ccatcaacaa aaatccaaca aataatatca
      781 tttatcctgc tgtaaaaaaa acgctatccc ggcgctgggt aacatcgggt tcatgctaag
      841 atagagcctt gtcccccgca ggattgatat ggggtgtttt cgatttcaga ttaccgatga
      901 ttcacgacgc ttatgacgaa gaaaaaagca cataaacctg gttcagcgac catcgcgctt
      961 aacaagcgcg cccgtcacga atactttatc gaagaagagt tcgaagcggg acttgccctg
     1021 caaggctggg aagttaaatc cctgcgcgca ggaaaagcca atatcagcga cagctacgtc
     1081 cttctgcgtg acggagaggc atttctgttt ggcgctaaca tcacgccaat ggccgtggcc
     1141 tccacgcatg tggtgtgcga tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa
     1201 ctggactcat tgtacggtcg cgtcaatcga gaaggctata ccgtagtggc gctctccctg
     1261 tactggaaaa atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac
     1321 gataaacgtt cagatatcaa agaacgcgaa tggcaggtgg ataaagcacg tatcatgaaa
     1381 aacgcccacc gttaaacctg cactccaatt attgaccagt tcctcaccgc gcctccctct
     1441 ccggcggcgc gaatgaacat cttattggct atcacatccg acacaaatgt tgccatccca
     1501 ttgcttaatc gaataaaaat caggctacat gggtgctaaa tctttaacga taacgccatt
     1561 gaggctggtc atggcgctca taaatctggt atacttacct ttacacattg gggctgattc
     1621 tggattcgac gggatttgcg aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa
     1681 aagccgcaaa aaatagtcgc aaacgacgaa aactacgctt tagcagctta ataacctgct
     1741 tagagccctc tctccctagc ctccgctctt aggacgggga tcaagagagg tcaaacccaa
     1801 aagagatcgc gtggaagccc tgcctggggt tgaagcgtta aaacttaatc aggctagttt
     1861 gttagtggcg tgtccgtccg cagctggcaa gcgaatgtaa agactgacta agcatgtagt
     1921 accgaggatg taggaatttc ggacgcgggt tcaactcccg ccagctccac cacttttgac
     1981 aggactgcaa ccggacagag actataaaaa cagtcactta cggacactga ccagacagta
     2041 agcggaccag aaaaagacaa aaatatacac gcaaaatgca cgtgcatttc aaaaaagcct
     2101 ctgattatga agtcagaggc ttttctagtt gtaaataagg gtaacaattt aacacgatgc
     2161 agcttaccaa ttacgtaaca tcaaacgctc taattgttcc caaatcctcc agaccactca
     2221 gctcctcttt catttcacgc tgacggcgat aaatctcatc gttgcgatcg acctgcgcct
     2281 gcaccattgc tgccgccagt tcttccagtt ccggcatcga cagtttcacc tgctgattat
     2341 cggcatcacc ccacgccata tgcgtttgtg ctgtgacaga ttttgccagc atgactaccg
     2401 gggacaggcg gcccagtgag tcggggccag cattccagat acgaccattc cattcaaacg
     2461 tgaacggctt cgcctcctgt tctgtgcgcc atgcttcaat ttcctgacgt ctggcctctc
     2521 tggccgcttc cagcatttct ggtgtcacag tgaatggggc tatctcaccc catttgccac
     2581 tttgcagttc ctgccagatt tgctgacccg tcggtgcgac atcatcagcg gtggctgtgt
     2641 aggggactgc c
//
LOCUS       DAESHI010000249         2644 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.252, whole genome shotgun sequence.
ACCESSION   DAESHI010000249 DAESHI010000000
VERSION     DAESHI010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.252"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            22..1104
                     /locus_tag="KW011_004409"
     CDS             22..1104
                     /locus_tag="KW011_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005099906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBH7662559.1"
                     /translation="MFRIPVNQWTLATAALGGVFLVSGLILLMNYNHPYTFTAQKAVI
                     AIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKTL
                     RAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDALV
                     KGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLIR
                     PGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGKL
                     TSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYSD
                     HFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1685..1996)
                     /locus_tag="KW011_004410"
     CDS             complement(1685..1996)
                     /locus_tag="KW011_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662560.1"
                     /translation="MADKAILWALISASTKEGRKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVNK
                     R"
     gene            complement(2085..2351)
                     /locus_tag="KW011_004411"
     CDS             complement(2085..2351)
                     /locus_tag="KW011_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HBH7662561.1"
                     /translation="MPQGVWSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLTQSC
                     LNAFGPSLGPRFCSEGCITGSSNDMQKLNELIFSEPDSTLTVTD"
     gene            complement(2367..2492)
                     /locus_tag="KW011_004412"
                     /pseudo
     CDS             complement(2367..2492)
                     /locus_tag="KW011_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
BASE COUNT          713 a          555 c          575 g          801 t
ORIGIN      
        1 ctggcgtggg cggtctttaa aatctttcgt attccggtga atcagtggac gctggcgacg
       61 gcggcgctgg gaggcgtgtt tctggtgagt ggtttgattt tgttgatgaa ctacaaccac
      121 ccttacactt ttaccgcgca aaaggcagtg atagcgatcc ctatcacgcc acaggtgacg
      181 ggaattgtta ctgaagtcac tgacaagaat aatcagctta ttcaaaaggg cgaggtgctt
      241 tttaagctcg acccggttcg ttaccaggcg cgagttgaca gacttcaggc tgacctgatg
      301 acggcgacgc ataatataaa gacgctgcgt gcgcagctca ctgaagcgca ggccaacacc
      361 acccaggttt cagcggagcg cgaccgtctg tttaaaaatt atcaacgtta cctgaaaggc
      421 agccaggcgg cggtgaatcc gttctcggaa cgtgacatcg acgatgcgcg gcaaaatttc
      481 ctcgcgcagg atgcgctggt gaaaggctcg gtggcggagc aggcgcagat ccagagccag
      541 ctcgacagta tggttaacgg cgagcaatcg cagattgtga gcttaagagc gcaacttact
      601 gaagcaaaat ataaccttga gcagactgtc attcgcgcgc cgagcaatgg ctacgttact
      661 caggtactga tccgcccagg tacatacgca gctgccttgc cgctgcgtcc ggtgatggtc
      721 ttcatccccg agcaaaaacg gcaaattgtc gcccaatttc ggcaaaactc gctgttacgt
      781 ctgaaacctg gcgatgatgc ggaagtggtg tttaacgcgc tacctgggca ggtgttccac
      841 ggcaaattga ctagtatttt acctgtcgtg ccaggcggtt cttatcaggc gcagggggta
      901 ttgcaatcat taacggtcgt tcccggcacg gacggcgtgc tgggaaccat tgaactggac
      961 cctaacgatg atatcgatgc cttacccgac ggcatttacg cccaggtagc agtttactcc
     1021 gaccatttca gccatgtttc ggtgatgcgg aaggtgctgc taagaatgac cagttggatg
     1081 cattatttgt atttggatca ttgaggggga agggggagat tatggaactt tgctccctgc
     1141 aagggctaat tatttttgca tattacttca ttggctgcga tgatttttct ttaatattca
     1201 ttatgttaat aagttctgga tgagtgtgct ctcattggat tttttatccg tcttgttctg
     1261 ccgcaggatg agactataca tcgtcgagat cgcaatgatg atatgagttg ttcctcctgc
     1321 gacggctcta atcaaaaaag agcaggagtg cttttccatc tattgcgatg atggctttat
     1381 tatctgaagg atgacagtca catttaaaaa atatttaatc tatagatcac aatgtagggt
     1441 catggaatat atgtatacaa ctattttctt cttcactttt aattatcgta ttcattataa
     1501 aactaagtta aaaaaactcc tagagagtga gttattaata tatttttaaa taaaattaag
     1561 ttatcattca tcgatgcatt ttatagaatc atcaatggcc ataatagtac tattaactct
     1621 caattttata tttttatcat cactacaaat attaactgaa tttatatcaa tgattttttt
     1681 attcttatct tttatttact gtttcaatgc aatcagcggc aagttggagt ggatttagat
     1741 ttttcagata tggtcttatg atctttccct tacttaataa atagcatgta taggattcgg
     1801 aaagtccagc atctattttg tacctcatta tattagataa ggatgtgaga aattccttgt
     1861 tatcattcgc agccatatat gccagtccga gttcagcctc tgcggcctta caggcaaaat
     1921 agcttagtga acacgctttt cgtccctctt tagtactggc tgatattagt gcccataata
     1981 ttgctttgtc tgccaaatct aactctctct gaaattgtct tgcagtaact gtactcgcag
     2041 aaatagagaa aaaaatacaa ttgttaataa ctttgccata ttttttagtc cgtaactgtt
     2101 aatgtgctat ctggctctga aaatattaac tcatttaatt tttgcatgtc attagatgaa
     2161 ccagttatac aaccctcgga acagaatcga ggtcctaaag acggaccgaa agcgtttaaa
     2221 caactttgtg ttaaaaatcc atcacgatta tatgtttctg tatttgctga cggtactaaa
     2281 cgtataccat taggttttct attggttgct ccaggaccat taacattcca gctccatacc
     2341 ccttgaggta ttggacctct attttgtaag tgtgtacaac caggattatt tttacattgc
     2401 atattattac cattattacc agaggcgaca ggaatagtaa gcacatcata agagttatca
     2461 tcattttgcg tatctgaaac gcatgaaagc atacttaaag ttatgctgta taaacaggtc
     2521 gctaatccca acgggtcaat atcagttatc ggattcaacg gatactgata aaaattccat
     2581 ccccccttca gtccaatctt ttcgcgccgg cgtgtacacc gggtccatct ggttttgcat
     2641 ctgc
//
LOCUS       DAESHI010000250         2586 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.253, whole genome shotgun sequence.
ACCESSION   DAESHI010000250 DAESHI010000000
VERSION     DAESHI010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.253"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            196..1149
                     /locus_tag="KW011_004413"
     CDS             196..1149
                     /locus_tag="KW011_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH7662562.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1190..2185)
                     /gene="pgl"
                     /locus_tag="KW011_004414"
     CDS             complement(1190..2185)
                     /gene="pgl"
                     /locus_tag="KW011_004414"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HBH7662563.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2445..2576)
                     /locus_tag="KW011_004415"
     CDS             complement(2445..2576)
                     /locus_tag="KW011_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HBH7662564.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVERMGSWPEVVMA"
BASE COUNT          714 a          635 c          578 g          659 t
ORIGIN      
        1 tatttttttc ataataatgc cctcaattta aaaacataac ttattgcgat atggttacat
       61 taagggcaaa gcatctctaa tgactttagc tcaacgatta ataacatttt ttcattaatt
      121 taagttggct aaatgcagtt aaaattttta acggccagcc acccaaaatc gtcgcttcca
      181 ttttccggag taataatgaa gcatgaatta tcaagtatga aggcatttgt catactggca
      241 gagtccagtt cgtttaacaa tgccgctaaa ttactcaata ttacgcaacc tgcattaacg
      301 cgaagaataa aaaagatgga ggaagattta catattcaac tatttgagcg tacaactcgc
      361 aaggttacgt taacaaaagc aggaaaaagg ttgctcccgg aggcgcggga attaataaag
      421 aaattcgatg aaacgctttt taatattcgt gatatgaatg cttatcatcg tggtatggtg
      481 acgttagcgt gtattccgac cgcagtattt tattttttac cgctggcaat cggtaaattt
      541 aatgagctgt atcccaatat taaagtgcgg attctggaac aaggcacgaa taattgcatg
      601 gaatcggtgc tgtgtaacga atcagacttt ggcataaaca tgaacaacgt cacaaattca
      661 tccattgatt ttaccccact ggtcaacgag ccgttcgtgc tggcctgtcg gcgtgaccat
      721 ccattggcca aaaagcagct tgtagaatgg caggagttag tgggttacaa aatgattggc
      781 gtacgctcct ccagcggcaa ccgactgttg atagaacaac agctggccga caagccctgg
      841 aagctggact ggttttacga agtgcgtcat ctttcgacgt cattaggact ggtagaggcg
      901 ggactgggga tttcggcgct tcctggtctg gcaatgcccc atgcgcccta ttcttcgatt
      961 attggtattc cactggtgga acccgttata cgtcggacat tggggattat tcgccgcaaa
     1021 gatgccgtgc tttctccggc agcagaacgc ttttttgcct tgctgattaa tttgtggact
     1081 gacgataaag acaatttgtg gaccaatatc gtcgaacgcc agagacatgc gttacaggag
     1141 ataggttaat acaccggcgc aggagagact gcgccgggta aatcagcggt tagtgtgcgt
     1201 taaccaccac ccacattggt ccctgcccga ccgcatagcg gcctttttca tgcagtagcc
     1261 cctgctcgcc aacaatttcg tataccgaga tgtggtgaga tttttgcccg gcggcaatca
     1321 gatacttgcc gctgtgatca acattgaagc cgcgcggctg ggtttccgtt ggctggaagc
     1381 cttctttact caacacgctg ccatcttccg aaacgctgaa aacggtaatc agactggcgg
     1441 tacggtcgca ggcgtataaa tgacgaccat ccggggtgat atgaatatca gccgcccaac
     1501 aggtgtcgga gaagttttcc ggcatcatat ccagcgtctg gacacattca atattgccgt
     1561 gtggatcttt cagttcccag acatccactg agctgtttaa ctcattgacg caatacgcat
     1621 attgttcgtt tggatggaat accatatgac gcgggccggc cccttcaacg gtagtcactt
     1681 ccgcagggtc ctgcgccacc agatgaccat catcgctgac cgtaaacagg caaatgcgat
     1741 cctgctttaa tgccggaacc cacagcgtac ggttgtccgg tgagatattg gcggaatggc
     1801 aaccgtccag cccctcgacc acatcgacga cgcccactgg caggccatct tccagacgcg
     1861 ttacgctcac gttacccgca ttgtaagaac ctacaaagac aaactgcccc tggtgatcgg
     1921 tggaaatatg cgtcggacta cccggcagcg cagactctgc ggcaaaggtc agtgcgccat
     1981 cgtccggggc aatacgatac gccaggacgc gaaactcagg gcgaacacca acatagagat
     2041 aacgtttgtc cgggctgacc accatcggct gcacctgccc cggcacatcg acaacctgtg
     2101 tcagcgtcag tgcgccttca tgattcaggt tccagacgtg aatttgctgg ctctcagggc
     2161 tggcgatata aactgtttgc ttcatgaaca cacctcaaaa atgataaatc ccttatcttt
     2221 catacacata acaacactta ctggataaaa aacagacaac aaaacacgat aaaatacaat
     2281 atcgcacatc aattaaatca ataagttagc actccatttc gaatggcata ttactaaaat
     2341 attagtcacc gacacgaatt tgacaatgcc acagatattt tcgcgactat tcagggagta
     2401 tgactctccc cgcttttgtt tacctgtatc aacgaccgta aacactatgc cataacaact
     2461 tctggccaac ttcccattcg ctcaacaatc aactcacttc cggcacaaat aaacgcatta
     2521 cgttgtagat cggtattctg gtcatttgtt gatacgcgta catagccaat aagcatggta
     2581 aatccc
//
LOCUS       DAESHI010000251         2572 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.254, whole genome shotgun sequence.
ACCESSION   DAESHI010000251 DAESHI010000000
VERSION     DAESHI010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.254"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            262..480
                     /locus_tag="KW011_004416"
     CDS             262..480
                     /locus_tag="KW011_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HBH7662565.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            482..847
                     /locus_tag="KW011_004417"
     CDS             482..847
                     /locus_tag="KW011_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBH7662566.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            844..1509
                     /locus_tag="KW011_004418"
     CDS             844..1509
                     /locus_tag="KW011_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662567.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVECWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1509..1874
                     /locus_tag="KW011_004419"
     CDS             1509..1874
                     /locus_tag="KW011_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HBH7662568.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVAPAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2329..2484
                     /locus_tag="KW011_004420"
     CDS             2329..2484
                     /locus_tag="KW011_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBH7662569.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRIGQTEVAVF
                     VDYESRE"
BASE COUNT          713 a          540 c          691 g          628 t
ORIGIN      
        1 gtttgactgg atgctctctg tggcaaaaag agaaaccaga ctggcagatg atgtaaggct
       61 attgcgccag gaggaacagg aaaggcggag aaatatagaa aagctgatac agattgagcg
      121 taacgcgaaa gcgcggatac gcagggcgac aaaatccaga actgaataat taaatttagc
      181 actgttaaaa atttaatcct taaccggagg gatttctgca ccctcaaatc atcaggagac
      241 cgctcgaaag ggcggtaatg aatggttaca ttatttagaa aaaaatatcc gcgaaagagt
      301 agaacaacag aattcctgtt tctcattctg tttatcgtgt tgatgacacc gatatccccg
      361 ctaatttttg tctggataat cggaaaaata attgagccag ttattgaatt gtataacgac
      421 gtggtatggg cgtcattcaa cacactgcac aataaaatta atccgtataa ggaaagctga
      481 tatggcactg acgaaaaaac aacgtgcaga actgcgcatg aagttcggtg gtcgctgcgc
      541 ttattgcggc tgcgaacttg gcgaaaagtg gcatgcagac catgtaaaac cggtcattcg
      601 ttttgatgga aatatgcttc accaggaacg tgacgatata tccaacatgg taccagcatg
      661 ccacccatgc aatctgcaca agcattgcag tagcctggaa gattatcgac gaattatcag
      721 tgatggtcgt cgtgaattcc ttgcgtccgg gaaaggcaaa gcgctggttc gtatgggatt
      781 ggttgaaatg aaatctgacc cggttgtgtt ctggtttgaa aaatatcaag aaggggctac
      841 ggcatgacca ctattaccaa agagcgactg caatggctgg ctaacatttc tggccgcgat
      901 gatattgacg atatagacgg cggtgaaatt cgtgaactgg cgcttatcgc tctggcatca
      961 ctggaagcag agcctgtagc ggaatgcatt gttgaagatg ggggcatgtg tattgacggg
     1021 ttcggtgagt atgtgggtca ctcgctgcct gatggaatgc atgagcttta tgctgccccg
     1081 cgaatgcgac aacctgatgg atggaaagcc gtagctgtag cgtggaaggt gacgtttact
     1141 caagttgacc aggaatctaa tacgttcact gctatatatt ttgacaaagc ggaagttgag
     1201 tgttgggtac gactgcataa agcatgtgat tttcgggcag atataacacc gctttacgca
     1261 gcgccggcag tgccggttgc agtaaacgac gacatggctt acgcattcca tcacgcactg
     1321 tcagattcat cgcttggctc tgatgaaatc gaagaaatta aaaccggttt gcgtgctgcc
     1381 tttgccaacg tcactatcca accagagccg atagtgccgg atgaaatcgg gccaaacgat
     1441 agtaatacgt ttgattatgt tgatggctgg aacgcctgcc gcgctgccat gcttaaggga
     1501 gataaataat gattaatcga accaaactgg agcacattct cgaatatgcc aggcagcaga
     1561 aatgtattgg gcaactttgt aaaattccac caggagatat ggttgaaatc gtggaaatgg
     1621 ccatgcgtaa ggctggcaac tctccggtag ctccggctgg ctggataagc tgtagtgatg
     1681 cagttcctgc tgaatattgc gatgtgattc ttctcgatga tctcgggaat gtattccccg
     1741 gttcctggga taaggttttt tgccccactc gtggcgggaa taagatggct tttgtggaca
     1801 aagacggcgt tgaagtagag agctcaactc actggatgcc gctaccggaa ccaccgcagg
     1861 aggtgaatca atgacctggc ctgaggcatt caccacggta ggaattgcga tggcggtggc
     1921 gctggtggtg tattcgattt gccgttgggg ttaacaaaca aaaaccccgg attgatggtc
     1981 cggggttttt gaaggaaaca aaacagaaac agcaattgcc gttacctgtt gttaccatgg
     2041 caagtaaacg tatctcaggc gagcgcattg cgccgttctg acgcagataa actagcctgg
     2101 atagatggtg ctggcaataa aaaatagcgt tttcttatcg gtgtcggtaa gattgctgcg
     2161 ggtgcttgag gctgtctgcc tcgggtatgt cactgtaagg cagacagaga aaagccccag
     2221 ttaacattac gcgtcctgca agacgcttaa cattaatctg aggcccaatc tatgtctcac
     2281 aaatgtaggt tagcctctta cgtgccgaaa ggcaaggaga agcaggctat gaagcagcaa
     2341 aaggcgatgt taatcgccct gatcgtcatc tgtttaaccg tcattgtgac ggcactggta
     2401 acgaggaaag acctctgcga ggtacgaatc cgaatcggcc agacggaggt cgctgttttc
     2461 gtagactacg aatctagaga gtaagagtga ccaggcggga gagtaatctc ccgccacctt
     2521 tgatgtgtca ggcatcctca acgcacccgc acttaacccg tttcggcggt tt
//
LOCUS       DAESHI010000252         2562 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.255, whole genome shotgun sequence.
ACCESSION   DAESHI010000252 DAESHI010000000
VERSION     DAESHI010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.255"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..796)
                     /locus_tag="KW011_004421"
     CDS             complement(<1..796)
                     /locus_tag="KW011_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HBH7662570.1"
                     /translation="MLQTFFLILCLSCYTASAGTDDNVGYIVGNSYGVGPSDQKWRET
                     GPNGDATVIFRYATSTNNLVFYKPTQLGPTGVKLQWSQLDTASGGGFLYCNRSDSTSG
                     SAMRIENAMVDSGKMYGSHKLFNTSVPGLYYTLLISNMWSAYGTVTNVSSPGIYIGDS
                     AEQYFSWYNPSEDVLYWSCNNANSTRKYWAVGGIYQTLTIEFYTDTNFDPTVTQQIKL
                     SSSSNYLYSFKAYGAGQGINEHSYFIKIDFDLLNVKLTNPTCFTAML"
     gene            complement(857..1453)
                     /locus_tag="KW011_004422"
     CDS             complement(857..1453)
                     /locus_tag="KW011_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBH7662571.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(1480..2082)
                     /gene="yadL"
                     /locus_tag="KW011_004423"
     CDS             complement(1480..2082)
                     /gene="yadL"
                     /locus_tag="KW011_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HBH7662572.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            complement(2097..>2562)
                     /locus_tag="KW011_004424"
     CDS             complement(2097..>2562)
                     /locus_tag="KW011_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBH7662573.1"
                     /translation="ATTCNITLTGNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADF
                     KLVASGCSSGISWIDTTLTGNASSSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATF
                     LKPNSAEKIRWSTDEMQPDKGLEMTVALRETDAGQGVPGNFRALATFNFIYQ"
BASE COUNT          712 a          552 c          513 g          785 t
ORIGIN      
        1 taagcatagc ggtaaaacaa gtgggattag tcaacttgac attgagcaga tcgaaatcga
       61 ttttgataaa ataactatgc tcatttatac cttggcctgc gccataggct ttaaatgaat
      121 acagataatt tgatgagctg gataatttga tctgttgagt tactgttgga tcaaagtttg
      181 tatctgtata gaattcaatt gtaagggtct gataaatacc acctacagcc cagtatttac
      241 gggtgctatt cgcattattg caactccaat ataacacgtc ttcgcttgga ttataccacg
      301 aaaaatattg ttctgcagag tcaccaatgt atatcccagg tgaactaacg ttagttacgg
      361 taccgtaagc tgaccacatg tttgaaatta ataatgtgta atacaaacca ggaactgatg
      421 tattaaataa tttatgggag ccatacattt tacctgagtc aaccattgca ttttcaatac
      481 gcattgcgct accacttgtg ctatcacttc tgttgcaata aagaaaacca ccaccagaag
      541 cggtatctaa ctgactccac tgtaatttta cacctgtagg tccaagctgc gtcggtttgt
      601 agaaaactaa attatttgta cttgttgcat atctgaatat gacagtagca tcgccattcg
      661 gccccgtttc tctccatttt tgatctgacg gaccaacgcc gtaactattc ccaacaatat
      721 atcctacgtt atcatcagtg ccagcactcg cggtataaca actcaagcac agaattaaaa
      781 aaaatgtctg aagaatttct tcattatcac tccaccatct actgctgtaa cagtcgcagt
      841 tatttgtttt gatctattat tcgtagtaca catcaactaa tatatgactc attacttggc
      901 cagatatgac atccagcgcg ggatcgattt tttgcattct ggcataaaat ttccatgaag
      961 aattatgcag ggcagaagtc attactggat acacggaacg agaatttcct gctgaactaa
     1021 taacattgaa tatattggtc gggtcctgaa tggaaaagag aacgacaccg acatttttag
     1081 ccccggttgc gacttcattg gcaaatattt gccttgaacc tgcagccatg acaccgctaa
     1141 gggctctaaa atcaatatgt aactgattaa gcacatcgcc agttccctgt aatgcacagt
     1201 ctgatactgt gacagtgaac tcttttccac ctggttgata gctttcagga gtaacattat
     1261 ctgaaaaata tcccacccca acgacgccaa gatcaatatt accctggtta ctgattccgc
     1321 ttttgcaagt gctgtttttt acattggcaa caaggtcaat atcctgccca gcgatggctg
     1381 gataggtaat gggaaactgt agtgacagaa acagaattat tctcttcatc agcttacgtt
     1441 gagtgggatg catatatagt tcctggatct gtcagtctgt tagttatagg ttactgtaaa
     1501 tgtggcaggt gcagaaaatt tacccgcagt gacattgctt acggttttat ttttttcgac
     1561 gaccagtcga gcactcatcg gataataaac aaccgcgttg gctgcattgg agatagtgac
     1621 ctcttgagat gctggtgtca cacaactaaa ttgtgttgcg ctccctgttg ccggagtttc
     1681 ggtgctccag acttcaacgg cgacggcact tgctttatct gcggctgtgg atccatttgc
     1741 gagccccgca ccgtcattag cagtaccttc gcatgtggct cgcttggtta attttatttg
     1801 tgctttttta ttggggatac ccgcacagtc tttgaatttg agtttgaatg ttttaacttt
     1861 ggtcttggca ttgatttcag aaatatatac atcgccaaag tcaacaacag aaatgtcctg
     1921 atcagagtca tttactaatg tagcggtaca agtacctatt tctacggtag taatcacggt
     1981 cagcccaatg ctgtctgttg ccgcataaga gggtacgctg aacaatgagg cagccaaaac
     2041 gacactgctg gataatccat aacgtaaatt tttaaatgtc atcttaatta tccttcttat
     2101 tgatagatga aattaaacgt tgccagtgca cgaaaattcc ccggtacgcc ttgccctgca
     2161 tctgtttcac gtagcgcaac ggtcatttca agacccttat cgggctgcat ctcgtctgtg
     2221 ctccagcgta tcttttccgc actgttaggt ttaaggaaag tggcatcatc agtagtccgt
     2281 tttttgaaac ccataccgat attacttgtc gtcgaagatg aatcaccaga ctgcggtata
     2341 ataagcttag gtgagcttga tgatgcattt ccggtcagag tggtatcaat ccaactgatg
     2401 ccactgctgc acccactagc aaccagttta aaatcagcct gagattctgt cgttttattc
     2461 gcgatcttat ccagacccat cttagggatt ctcaatgtgt agttattatt gccatcattc
     2521 gtgacgttat taccagtaag tgtgatgtta caggttgtcg ct
//
LOCUS       DAESHI010000253         2556 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.256, whole genome shotgun sequence.
ACCESSION   DAESHI010000253 DAESHI010000000
VERSION     DAESHI010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.256"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(58..261)
                     /gene="tatE"
                     /locus_tag="KW011_004425"
     CDS             complement(58..261)
                     /gene="tatE"
                     /locus_tag="KW011_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HBH7662574.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(390..1178)
                     /locus_tag="KW011_004426"
     CDS             complement(390..1178)
                     /locus_tag="KW011_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HBH7662575.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSWIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            1271..1654
                     /gene="crcB"
                     /locus_tag="KW011_004427"
     CDS             1271..1654
                     /gene="crcB"
                     /locus_tag="KW011_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HBH7662576.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="KW011_004428"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="KW011_004428"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBH7662577.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(2092..>2556)
                     /gene="pagP"
                     /locus_tag="KW011_004429"
     CDS             complement(2092..>2556)
                     /gene="pagP"
                     /locus_tag="KW011_004429"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HBH7662578.1"
                     /translation="TFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPW
                     GGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTA
                     GVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          653 a          659 c          580 g          664 t
ORIGIN      
        1 tttgaagcgg tttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca
       61 ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc
      121 atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag
      181 cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt
      241 ggtaatacta atctccccca tagatacctt cttgacatat aaccggcaac agaacgcgcc
      301 ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca
      361 aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg
      421 ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc
      481 ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc
      541 cagctttgcc cgatattttt gttcccgcac tcccccgccg ccaccatata acaggtggta
      601 tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcgg cccgcgaacc
      661 caggcggcag gaagtaccag gatttcagct ccctgtaatg cctgtgccag cgccagctct
      721 ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccctccac ctcgagtaac
      781 ggagcgattt cattaccagc atcaacacgg cgtgattcct gaatggcaaa tgcatcatag
      841 agatgcaatt tggcataacg ggcgacgatg tttcctgcct gtagcgccac cagcatattc
      901 catgcgcgcc ccggcgttga aggaacatga atcgtcagaa ttgtcgtcat catgttacgt
      961 ttactttctc gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc tgatttaacc
     1021 gatagatctg catcatgatc atcgcgcgcc agcaatgctt ccggcaggac gaacagcgat
     1081 acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt cttttcccac
     1141 acagatgtaa cagcaaactg tcctgcggca accagcattg ccacctcctg tcagagtcgt
     1201 aaaattcgcc aacgacgatt attcggcgtt acacttatca ctcatacaaa tcaaatagca
     1261 ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta cgggaagcgt
     1321 ggcgagatgg ctgttaagta tgcgatttaa cccgctgcat caggcgattc cgttggggac
     1381 gctggcagca aacctgattg gggcattcat cataggaatg ggattcgcct ggttcagcag
     1441 gatgacgaac attgatccag tgtggaaagt attaatcacc accggatttt gtggcggtct
     1501 aacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg gccgctttgg
     1561 ctgggcgtta ctgaacgttt tcgtcaacct tctggggtct tttgccatga ccgcactggc
     1621 tttctggctg ttttcggcct caaccgcaca ctaaagacca aaaaaaaccc gctgattaag
     1681 cgggttttga attcttgctg acgtatctta cagagcgatt acgtttgcag cagaagggcc
     1741 tttggcaccg ttagtgattt cgaactctac gcgctgacct tcagcaagag ttttaaaacc
     1801 attagtctgg attgcagaga agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat
     1861 gaaaccgaat cctttggact cattaaacca cttaacgtta cctttaatct tagacatcaa
     1921 aattaccttt acatgaaaaa tagacacaaa tgctgtgtcg gttaccagta caccaattgt
     1981 ggtacgcttt gtccagtgca actttactaa aaagtgataa aagtcgcgtt atttttgatg
     2041 ttacttattt tagttattga ttttaaagaa gttactaaaa cttcatttgt ctcaaaactg
     2101 aaagcgcatc caggcaaagt acacattgcc attgttgtag gtacccggaa tgtaggtcat
     2161 ctgaaaagtc actgggccat aacccacgga ggccaatggc agtagaaccg ggagagggat
     2221 gtaattccag ttatcgcgtg ccgttacgcc agcggtgaat cccagaccta aatgaaaatt
     2281 ttcatccgcc agcggtcgcc aggtactttc ccatccgtat ccggcaatcg gttcccattt
     2341 gttccacgag tccttaaatg ccatggcata caggccatgc cagtttcctt tttcatccca
     2401 tcgcgactga ccaaaaccgc caccccacgg tcgctcgtta tagcgatcgg ttttttcttt
     2461 gtcgtaagcg aaacgtgcat gccaggtgat ggcaggaata tataaatcat aatgttcagg
     2521 ctgttgccag gtttgtgcaa tattttctct aaacgt
//
LOCUS       DAESHI010000254         2545 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.257, whole genome shotgun sequence.
ACCESSION   DAESHI010000254 DAESHI010000000
VERSION     DAESHI010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.257"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..159
                     /locus_tag="KW011_004430"
     CDS             <1..159
                     /locus_tag="KW011_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005087616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662579.1"
                     /translation="DAKRLQQLEPNAGTGARIWLAKEALESGDYDSEEAFYKAEGRTG
                     YSPGLGGV"
     gene            828..1673
                     /locus_tag="KW011_004431"
     CDS             828..1673
                     /locus_tag="KW011_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBH7662580.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPKSFTAHNRAQAVFLC
                     VMHSHIQIMVGRAGQSQDWPGSVMTGCSNPVRLTTHEIATSGGELIKFIAEAAIMATT
                     LSHPCVTVENGRAVTTSVAVAEFFHKRHDNVLRAIANIECSDKFTALNFEASEYTDSI
                     GRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMIPG
                     DGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVEQL
                     LKIHC"
     gene            1684..1872
                     /gene="dicB"
                     /locus_tag="KW011_004432"
                     /gene_synonym="ftsT"
     CDS             1684..1872
                     /gene="dicB"
                     /locus_tag="KW011_004432"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HBH7662581.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            1869..2060
                     /locus_tag="KW011_004433"
     CDS             1869..2060
                     /locus_tag="KW011_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HBH7662582.1"
                     /translation="MNTVFALVLTVFLVSGEPVDMVTGVYSSMKECMTAAAKQKIPGD
                     CYPVDKVIHHDNNEIPAGL"
     gene            2145..2420
                     /locus_tag="KW011_004434"
     CDS             2145..2420
                     /locus_tag="KW011_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662583.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
BASE COUNT          743 a          556 c          599 g          647 t
ORIGIN      
        1 gatgcaaaac gcctccagca actggagcca aatgcaggca ctggggcacg catctggctg
       61 gctaaagaag cgctggaatc tggcgattat gatagcgaag aagccttcta caaagcagaa
      121 ggccgtacag gatattcacc gggtcttggc ggggtataaa taccatgcgc attgactgaa
      181 ttcgcaaaca aaaacagacg cgcgatacct ggatagtcgg tctgcatagt cagatatctg
      241 gctgcgaatt tgataaatat caccgccctt ttcggaaaca taagttccgt ccgggagttg
      301 attatacgaa ccatctccat gggggatcgt tctcctgaat cctagtgaga gcatatattt
      361 atgaagccct tcgtaatcct ctggctcagc attatatagt tctactctgg cgagatacgt
      421 tggcatattc atttccttac tggttgtgtg agaacttcag taaagatacc accaaagccc
      481 ggaggtggtg aaataaaacc gggcacaaca cgaaggcgca tttccggtat ccataaagag
      541 tcggtcttgt ctgttaaatt taaatggtgg gagtgcgcct ccggttgtaa ataacgacat
      601 tgctgtgtga agtaccagtt ggcggcatcg gtttaattgc tggctgatgt ccgccctttt
      661 taaagtgaat tttgtgatgc ggtgaatgcg gctcagcgca cgcggaacag ttaaaacagt
      721 aaggaaaatc ccttattccg gcgttaattg ttaactggtt aacgtcacct ggaggcacca
      781 ggcaccgcat caacaaagtt cacttcggtg atgaaaggaa agagaaaatg ttgaatgtag
      841 ctattgaaaa ccagaacggg tggaattata gtgcacctgc acctcataaa acgggtgccg
      901 ggattgctac cccgaagtca ttcacggcgc ataaccgcgc tcaggcggtt tttttatgcg
      961 taatgcacag ccacattcaa attatggtgg ggcgtgcggg gcagtcgcaa gactggccgg
     1021 gttccgtgat gaccggttgt agcaaccctg tacgtctcac cacccatgag attgctacct
     1081 ccggtggtga gttaatcaaa ttcatcgcgg aggctgccat catggctact accctttctc
     1141 acccttgtgt aaccgtagaa aacggtcgcg ctgtcactac gtctgttgcg gttgcagagt
     1201 tcttccacaa acgacacgac aatgtgttac gtgccatcgc aaatattgaa tgctcagata
     1261 aatttactgc cctcaatttt gaagcgagcg aatacaccga ctcaatcggg cgcaaactcc
     1321 caatgtacca aatcaccaaa aacggcttcg ttttcctggt tatgggcttc actggcaaaa
     1381 aagccgctgc attcaaagaa gcctacattg ctgagttcga ccgcatggag gccgaactgc
     1441 gccagaataa caccccacct gctgacaaga tgattccggg tgatggtcgc actctggttg
     1501 ttcacttcga caaattcggc aatgtcgaat tcaccgaaac cgttcctgat ggcgcactgg
     1561 tctgtaccct ggagactttc cggttttatc tggaaaaaca gggatggact cttgttaacc
     1621 ggggcgcaat taaaaatatg actgtggaac aattactcaa aattcattgt tgaggacgca
     1681 ataatggaga cgttattacc aaacgttaat acgtctgaag gttgttttga aattggtgtc
     1741 actatcagta accctgtatt tactgaagat gccattaaca agagaaaaca cgaacgggag
     1801 ctattaaata aaatatgcat tgtttcaatg ctggctcgtt tacgtctgat gccaaaagga
     1861 tgtgcacaat gaatacagta tttgcactcg ttctgacggt ttttcttgtt tccggtgaac
     1921 cggttgacat ggttactggc gtatacagct caatgaaaga atgtatgact gccgcagcga
     1981 aacagaaaat tcccggtgac tgttatccgg tcgataaagt tattcaccat gataataacg
     2041 aaatcccggc aggtctttaa aacagttccg taataaatat ccggtttcat tcttatatgc
     2101 cagcaatggc aggggttcgc tcaaccttaa ttaaggagaa aaacatgatt accagttatg
     2161 aagccactgt tgtaactact gatgacattg ttcacgaggt taatctggaa ggaaagcgta
     2221 ttggctacgt gattaagaca gaaaataaag aaaccccatt cactgtggtt gatatcgacg
     2281 gtccatcagg caacgttaaa acacttaacg atggtgtaaa aaaaatgtgt ctggtgcaca
     2341 taggaaagaa tctgcccgca gaaaaaaaag ccgaatttct ggcaactctg attgcaatga
     2401 aattaaaagg tgaaatctga aaaaaagaaa gcctgcacac tgtgcaggcc tgagtgaaga
     2461 acctgggaca tttattcatc actcgcagta attttaatct gagttgaggt taaaaaacaa
     2521 tgagcaccga taaacaagtt taccc
//
LOCUS       DAESHI010000255         2540 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.258, whole genome shotgun sequence.
ACCESSION   DAESHI010000255 DAESHI010000000
VERSION     DAESHI010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.258"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(107..1525)
                     /gene="phrB"
                     /locus_tag="KW011_004435"
     CDS             complement(107..1525)
                     /gene="phrB"
                     /locus_tag="KW011_004435"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HBH7662584.1"
                     /translation="MTTHLVWFSQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGVGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPIIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDLEGEFIRQWLPELRNVPGKSVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(1522..2031)
                     /locus_tag="KW011_004436"
     CDS             complement(1522..2031)
                     /locus_tag="KW011_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HBH7662585.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
BASE COUNT          642 a          659 c          579 g          660 t
ORIGIN      
        1 caggcctacg tatttcctgc aatttattga atttgcacaa atttgtaggc tggataaggc
       61 gttcacgccg catccggcat ctgggctcga ttgcctgacg cgtctgttat ttccccttcc
      121 gcgccgcctc atacgctgcc aacgtttgta ctctcgcctc tttgtgctcg actatcggtt
      181 gcggataatc cagcttcaca cctgctttct gcgcccactt ccacggctca tgcaccgatt
      241 tccccggtac attgcgtagt tccggtagcc actggcggat aaactcacct tcgagatcaa
      301 atttctcgcc ctgggttgtc gggttgaaaa tacgaaaata cggcgctgca tcggttccgg
      361 ttgaagcggc ccactgccag ccaccgttat tggctgccaa atcaccatca atcagctgcg
      421 acatgaaata tcgctcgcct tcgcgccagt cgatcaataa atctttcacc agaaaactgg
      481 ctgtaatcat ccgtagcctg ttatgcatcc agccagtgct gttaagctga cgcatagcgg
      541 catcaacaat cgggtatccc gttttgcctt cctgccaggc ctgtaaatgt gcgggattgc
      601 tctgccactg tacacgatcc gtccaggcaa taattggacg atgtttacac aacgaggggt
      661 gatacgttat caggtgacgg taaaactcgc gccagatcag ctcattaagc cagacactac
      721 cgaccccacc gtccagcgcc tgcggctgtt cagccaacaa gcgatgcaag cactggcgag
      781 gcgataaccc gcccgttgcc aggctggccg acaaacggct ggtgccttcc actgccggaa
      841 aatctcgttg ttgctcatat tctccggcac cgttctggca aaactggcgt aattgcgcaa
      901 tcgccgcttt ttcttccacc ggaaaatgcg cagtatcgaa agactgacga ggataattca
      961 gcgtaatgga tggcgagggc tctatcgatc cgctactacg aacttttggc gcagcgacgc
     1021 actccggcat cccttcccgc agccgtttca gccaggcatt cttaaaaggc gtaaagactt
     1081 tgtacatctc gtgattaccg gtcatcaccg cgccaggcgg caggatcacg ctgtcatcaa
     1141 atccttcaca caccacgtta cgcagcgctc tttcaacttc cacatcccgc gcccgctcat
     1201 tcacttcata ctgatagtta taaaacaggt gggtaacgct gttttccgcg cacacctgtt
     1261 taactatttc gacactggcg acaaagtcat ccacttcacg gaacaataaa ggaatacctt
     1321 tttccgcaag cgctatttgt agcccattca gttgagcatt gataagttca gcctgacgcg
     1381 gcgacatgtt atgcgtcgcc cactggcgtg gtgtagcgat atacaacgcc agcacgcgtg
     1441 cagacgaatt gcggcaggcg gcagccagtg cgagattatc gtgcagacgt aaatcttggc
     1501 taaaccagac cagatgggta gtcataaaac tcctgaatgg cgcaagttaa tcacacgcgg
     1561 ccaaagttcg aaataacgct gccctgaaag ccaggcgtca tgataaagcg ccatatagtg
     1621 tttgatacgc atcagcggcg caagaagtgg ttgctcgcca cggcgataac tgtcgataag
     1681 cgcagccagc tgctggcgtt ctgtggaatc aatatgcggg cgaaaataac cctgaacgtg
     1741 catcaaaaca ttggtgtgat cgcgcgggtt ggcggggtga gaaagcaaaa caactacgcg
     1801 ttggcggtac tggttgtaat agtcatccag attttgccac tggtgaatat cagccacgaa
     1861 cgggccaagt ttgcggtact ccggctgaga atgcgccaga aagacgagtt tatagcggct
     1921 gtgataatcg agaagcgccc cgcgcgttaa gccatgttct ttcagtcgat gtaactcatg
     1981 aagggcaaag atacggatta gggtgctgtc atcaaatcgt tgtagattca ttactgctcc
     2041 tgaacggtat aaaggagtaa gtgtagacga tggtaaatga attttatggg gcagagtttt
     2101 tgcggggaag tattttgttg gtaaaaatat tatcaacttc atctgagtga gaattattct
     2161 actatttccc gagctgcatt cattttttgt attttttatt tttagaaaat aacagttctc
     2221 aaagcttaat tacctcacta ttaattgtat ttcaaactaa caatcgcact ccattttctc
     2281 tttttaaaaa acataaacac tgttaaaaca aatacttatt tcgttatctt aaatgctatt
     2341 tctacatcac tcgtccttat aatcaaatta ttcttattca tagctatttt tgcaggttta
     2401 aaatagcggc tttcaccaaa gtcagccatg aaaattagac tacaagtcag ggcaagatta
     2461 cgaaagcccg ctccccgcaa ggactgacgc cagatagttt ctgtccatgg ctgcttttcg
     2521 catcttacgt cttaaccctg
//
LOCUS       DAESHI010000256         2089 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.259, whole genome shotgun sequence.
ACCESSION   DAESHI010000256 DAESHI010000000
VERSION     DAESHI010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.259"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(60..1025)
                     /locus_tag="KW011_004437"
     CDS             complement(60..1025)
                     /locus_tag="KW011_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBH7662586.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="KW011_004438"
     CDS             complement(1879..>2089)
                     /locus_tag="KW011_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662587.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          577 a          550 c          436 g          526 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcaccccc gaacggctta ccccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattttc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aatcaccgct tcacttctt
//
LOCUS       DAESHI010000257         2088 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.260, whole genome shotgun sequence.
ACCESSION   DAESHI010000257 DAESHI010000000
VERSION     DAESHI010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.260"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            81..1253
                     /gene="istA"
                     /locus_tag="KW011_004439"
     CDS             81..1253
                     /gene="istA"
                     /locus_tag="KW011_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HBH7662588.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1253..2050
                     /gene="istB"
                     /locus_tag="KW011_004440"
     CDS             1253..2050
                     /gene="istB"
                     /locus_tag="KW011_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HBH7662589.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVESGQHQ"
BASE COUNT          552 a          505 c          590 g          441 t
ORIGIN      
        1 acggtaattc gccatttgga ttgtccgctc cacccaacat gttgtttcct taaggttctc
       61 acaccagaaa ggacatcaac atgctgagca gagaggactt ttacatgata aagcaaatgc
      121 gccagcaggg cgcgtacatc gtcgatattg cgactcagat tggttgctct gaacggacgg
      181 tcagacgcta cctcaaatac cctgaaccgc cagccagaaa gacccgccac aaaatggtta
      241 agctgaaacc gtttatggat tacatcgaca tgcgcctggc agagaatgtc tggaatagcg
      301 aggttatctt cgcggagatt aaggcaatgg gttatacggg cggacgttcc atgctgcgtt
      361 actacatcca gcccaaacgt aaaatgcgtc cgtcaaaaag aacagttcgc ttcgaaactc
      421 agcctggata ccagctccag catgactggg gcgaagttga ggtggaggtt gccgggcaac
      481 ggtgcaaagt taactttgcg gttaatacgc tggggttctc ccgcagcttc catgtcttcg
      541 ccgcaccaaa acaggatgct gagcatacct acgaatcact ggttcgcgcc ttccgctact
      601 tcggtggttg tgtgaaaacg gtgctggttg ataaccagaa ggctgcggtg ctgaagaata
      661 acaacgggaa agtcgtgttc aactccggat tcctgttgct ggctgaccac tataacttcc
      721 tgccacgggc atgccgtcca cgcagggcca gaacaaaagg taaggttgag cggatggtga
      781 aatacctcaa ggagaacttc ttcgtccggt accgcaggtt cgacagcttc actcatgtca
      841 atcaacaact ggagcaatgg atagccgatg tggctgacaa acgggagctt cgccagttca
      901 aagaaacgcc ggaacagcgc ttcgcgctgg agcaggaaca tctgcagccg ttaccggata
      961 cagacttcga taccagttac ttcgatatcc gccatgtgtc ctgggacagc tatatcgagg
     1021 ttggtggtaa tcgttacagc gttcccgaag cgctgtgtgg tcagccggta tcgatacgaa
     1081 tatcgctgga tgacgagttg cggatctaca gtaatgagaa actggtggcc tcacatcgcc
     1141 tctgttcagc atcgtctggc tggcagacag tgccggagca tcacgccccg ctctggcagc
     1201 aggtcagtca ggtggaacat cgaccactga gtgcctatga ggagctgttg tgatgcatga
     1261 actggaagtc ctgctgagtc gcctgaaaat ggagcatctg agttatcacg ttgaaagcct
     1321 gctggaacag gcagctaaaa aagagctgaa ctaccgggag ttcctgtgca tggcgctaca
     1381 gcaggaatgg aacggcaggc atcagcgcgg tatggagtcc aggctgaagc aggctcgtct
     1441 gccgtgggtc aaaacgctgg agcagttcga ctttaccttc cagccgggca tcgaccgtaa
     1501 ggttgtccgg gaactggctg gtctggcgtt cgtggagcgc agcgaaaacg tgatcctgct
     1561 gggccctcct ggtgtcggaa aaactcatct ggccatagct cttggcgtga aagcggtgga
     1621 tgcgggacat cgggtactgt ttatgccact ggacagactg atcgcgacac tgatgaaagc
     1681 gaaacaggaa aaccggctgg agcgtcagct acagcaactg agttatgccc gggtgttgat
     1741 cctggatgaa ataggctatc tgccgatgaa cagagaggaa gccagtctgt tcttccggct
     1801 actgaaccgt cgatatgaaa aagcgagcat catactgacg tcaaacaaag ggttcgcaga
     1861 ctggggagaa atgttcggag atcacgtact ggcaacagcg atcctggatc ggctgctaca
     1921 tcactcaacc acgctgaata tcaaaggaga gagttaccgg ttaaaagaga aacgtaaagc
     1981 tggagtgctg accaaaaaca caacgccaat cagtgatgat gaaatggtgg aaagcggaca
     2041 gcatcagtaa cgaaagtatt tagcgggcat gaaaatggca aataacgg
//
LOCUS       DAESHI010000258         2005 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.261, whole genome shotgun sequence.
ACCESSION   DAESHI010000258 DAESHI010000000
VERSION     DAESHI010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.261"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..389
                     /locus_tag="KW011_004441"
     CDS             <1..389
                     /locus_tag="KW011_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662590.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(414..752)
                     /gene="maoP"
                     /locus_tag="KW011_004442"
     CDS             complement(414..752)
                     /gene="maoP"
                     /locus_tag="KW011_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HBH7662591.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            871..1710
                     /gene="hdfR"
                     /locus_tag="KW011_004443"
     CDS             871..1710
                     /gene="hdfR"
                     /locus_tag="KW011_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HBH7662592.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1806..1881)
                     /locus_tag="KW011_004444"
     tRNA            complement(1806..1881)
                     /locus_tag="KW011_004444"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Trp,seq:cca)
     gene            complement(1890..1966)
                     /locus_tag="KW011_004445"
     tRNA            complement(1890..1966)
                     /locus_tag="KW011_004445"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1930..1932),aa:Asp,seq:gtc)
BASE COUNT          529 a          483 c          487 g          506 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc
      361 tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatca
      421 tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga
      481 aaacgttttg gacgcttaat acgcgtcata tacttggacc acacgcgttc tgcttctgtt
      541 actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc
      601 tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc
      661 tctttgatgg tgaaatcacc atgacgagag aatccacgtg gataatgttt attgtcgaaa
      721 tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag
      781 ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc
      841 ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg
      901 gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca
      961 gcagtgagct ttcgaatcag gcaactggaa aatcaactgg gtgtgaacct tttcacccgc
     1021 cacagaaaca atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg
     1081 ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg acataacgag
     1141 ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg gttgggacgc
     1201 ttgtatcaaa atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg
     1261 cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg
     1321 cccaaaatgg acgaatttag tagtcagttg ctggggtatt tcactttagc gctttatacc
     1381 agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat
     1441 tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct gacaaccagt
     1501 tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtt
     1561 agctgggcgc gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg
     1621 ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg cgatcttttg
     1681 aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa
     1741 ggatttactt cggagaggat tatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1801 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
     1861 taccaattga actacgcccc taattagggt ggcggaacgg acgggactcg aacccgcgac
     1921 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta
     1981 caaccaccgg ttttatgacc ggctt
//
LOCUS       DAESHI010000259         1982 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.262, whole genome shotgun sequence.
ACCESSION   DAESHI010000259 DAESHI010000000
VERSION     DAESHI010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.262"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(110..1099)
                     /locus_tag="KW011_004446"
     CDS             complement(110..1099)
                     /locus_tag="KW011_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBH7662593.1"
                     /translation="MKNPKLQYLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGA
                     RMASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPR
                     IVADYPDARWMFLTLTVRNCAIGELGEMLNRMNAAFQRMKVRKEFLPVQGWIRTTEVT
                     RGSDGSAHPHFHTLMMVPPSMLSGDGYVKHARWVGLWRECLRVSYNPNVDVRAVKPRK
                     PKDGESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGAL
                     KDVLKLDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESG"
BASE COUNT          411 a          597 c          537 g          437 t
ORIGIN      
        1 aaaaaaccga gaaacggcgg gaattttcgg gacaggatgt gggaatgtcg gaccgcgcac
       61 gctcgctcag acattcccca cctggtcagg gcttcgcccc gacaccccgc tagcctgatt
      121 cagttttatc tttcgtcggt gagcgacggt actttctgac ctcggttttc cactcaaacg
      181 cgattcgtga accgtcatcg tcgccctcgg aaatatcatc cccgatcacc atgtcggcgt
      241 cggtttcctg atcgagtttc agcacgtctt tgagtgcgcc gccggtcgcc acaaagcggc
      301 gcttgtgcgt ctggcgggtc agttcgagga accattcagg atcggccacc atgtcagccg
      361 gcttcgtgga atatttcagg gtttcggcca ctgcgccgcg caccagttca gcggtcgcgc
      421 aggctaagga ctcgccatcc tttggcttac ggggttttac tgcccggaca tcaacattcg
      481 ggttgtaact gacccgcagg cactcccgcc agagtccaac ccagcgagca tgttttacgt
      541 acccgtcacc agatagcatt gagggcggca ccatcatcag ggtgtggaag tggggatgcg
      601 cgctgccgtc gctgccgcgg gtaacttccg ttgtgcggat ccagccctga acaggcagga
      661 actccttgcg aaccttcata cgctgaaaag ctgcattcat ccggttcagc atctcgccca
      721 gttccccaat cgcacagttg cgcacggtca gggtcaggaa catccagcgg gcatcagggt
      781 aatccgccac gatacgcggc agggactggt agaaacgggc ttgccacatc agggagcgcc
      841 tccactggca gacaggacag tgccggacac ggcagaaatg cgcctcacgc agcctcagac
      901 gcgtttctcc ggtttccttg agggtagacc acccgaaacg cagtaaaccg ccacaggagg
      961 ccatacgagc cccgtagcgc tcatattcgg ccgcaagtag gtaaatccct cccacgtcgt
     1021 cagaaacgct tctgtgtgcg tcccagggcg cgtcagaggg ggaataatca gtgaggtact
     1081 ggagtttagg gtttttcatg cgcaccaccc gattgtgggc gatggctcat ctgacgtgtt
     1141 atcattgcca tatcacctgt gttgtcgtgt cactaattcg aatttctaca ggtggtcacg
     1201 cccccgaacg attgccctcg ttgcgggggt cgcttttaac gcgaccactt ccgacagaat
     1261 acttctaaac ccatttcgga gcaaatgtgc cctcaagcct tgcgggacaa gggctggagg
     1321 ccaatttgaa tcctagagtt atcccttaag tataaataca aggatcgcgc gcgagatttg
     1381 cttgaatttt gagctggatt ttcagtaacc caaaacctcc caaccccacc cacttacacc
     1441 catctcatcc acagactttc accccgattt tgtgaacaac aaaaaaccgt cttgcaggtg
     1501 gttggcctca ctccgtgaca ccaaccacca tccccagcgt tttccccgtg ccgtcgtcaa
     1561 tcacaaaatc ggaacccacg ccggaggcgc ggggccccgc gattttctgt ttgacgaatt
     1621 cgggaacggt gagcggtacg cggaattttt tcgcgtgccg tgaggcgttg gcggggtgtc
     1681 ggggcgaagc cctgaccagg tggggaatgt ctgagcgagc gtgcgcggtc cgacattccc
     1741 acatcctgtc ccgatttctg cgggtttgtg tcaccggaac actggcgcaa tgtcggcagt
     1801 ttttgcgccg cgccgccctc cacgtaagcg cacagttctg gcaaccggac agcaaatcat
     1861 tctgccgttt tcgtgccaca gctgttccgc gcgatcgaca aactggcagt cggggaatct
     1921 cacgaagtgc gcactcatac aaactacact tcgtttcgtt tgtcgtgcgg tctgcgacgc
     1981 tc
//
LOCUS       DAESHI010000260         1979 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.263, whole genome shotgun sequence.
ACCESSION   DAESHI010000260 DAESHI010000000
VERSION     DAESHI010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.263"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(358..1401)
                     /locus_tag="KW011_004447"
     CDS             complement(358..1401)
                     /locus_tag="KW011_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBH7662594.1"
                     /translation="MNDRLCFEVHDNKGYFVFSDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            complement(1812..>1979)
                     /locus_tag="KW011_004448"
     CDS             complement(1812..>1979)
                     /locus_tag="KW011_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBH7662595.1"
                     /translation="LHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
BASE COUNT          575 a          404 c          425 g          575 t
ORIGIN      
        1 ccacgggaat atctgcggca gcgggcttgt aatgggttaa gtgataacag atgtctggaa
       61 atataggggc aaatccacgg ggataccagt tcaaccgaaa acgccagagg aggggattac
      121 ccgctggcag ggtaaatctg tggtttttga tgccagctat aacgtgtttg acgataaagt
      181 attacgaaag aactttaatg agttcatgga gtaattcgtt aaagtaattt acaacgctat
      241 ccttttgcaa aggaaacttt cagaaaataa ccccagggta atcacctggg gaaggaacac
      301 agtgttatta ttggataata aattattact tgttttaaag ttacactaat gcaatatcta
      361 ctgatactgg atgcttgaat gtttcatccc tgctgcggaa aagggcaata tccagggcca
      421 gatatattca ccgttcatat ttctgcattt ctgtacaatt tcctcagaaa gtgtcttgtc
      481 aattcttttc cacaggtgct cctgctgtct taaaatactt tcgcatattt cgaaatcatc
      541 ttcctgaatc agcatttcgg cgcatttaat gatttctgat cgttcatgca gcacggagga
      601 atggttatag agccagttta caaacagcaa tgcttccgga tattgtcccc ataatggtga
      661 gtatgttgca tagtaatctt ctgccgtatc cagagagccg gagaagtcat gccacacagc
      721 aagtggaaat ggatgcagat ttccgcacag catttctgcg atgtaagtgt ttgtggcaaa
      781 gccatggcga aaagctgtgg cggccttaac atgttccccg ttcagaaaat gcaggagacc
      841 gagttcatac cagtaaggtg aaaattcatc agcatgctct ttcagtacac tgaaggctcg
      901 ctcatgatcg ccggtgcgta acagctctga acccaacaac cagcgagccc cctgattatc
      961 ttcaggattg tatgccagta ttttatcagt gagcataacc gcatcctgat ggcgctgtaa
     1021 gtgcacattt gcgagaattg ctgcataaag cgcgcgcaga taaggccggt tttcgggatg
     1081 catccagatt atttcaccac agaaactttc cgggatgata cgattacctg ccgccagtcc
     1141 tttaagagag gcgttcagtg ccttacgcgg ggcattctgt tccagaaatg catatgccag
     1201 atgagcgtgt atatcaataa agtccggctc ccgttgagca aggcgtcgga gtttgttgat
     1261 gtaactcgtc tcgctgattt catcagcgtc gtatgcatca agcacttctt caaactcccc
     1321 cagcaatggg ccaaaccagg tatccgaaaa tacgaaataa cccttgttat cgtgcacttc
     1381 aaagcataat ctatcgttca tttctgagcc tgtaaaacat gtggtgatat agtctgagag
     1441 cataaaagtt gagattctgt gtatcaagtc ctgtcttggc accgaatgaa tattgcttac
     1501 gaagcccctt catgctttcc ttgagaacaa taattttatc tttatgtgaa tctcattttt
     1561 tataatgatg tcagatgatt gaaatgctaa attatatctg aatcaacttt tcctttgcta
     1621 aaggtatcga aataactgca aggctgtatt ttgtaattgc caatttaaaa atgtcaattt
     1681 gtttttaatt atgtttttgt aataagtttg aagacgcaaa aaaataatat aataacaaaa
     1741 atcctccagc cttgtaggcc agaggataaa atacttaatt gaggaaaata tcagggaaaa
     1801 gtatttctga cttaactttc ccggctggag agtgaatcaa tgtaatcagc gtaccattgc
     1861 atcatttcac ggcggccatc gagatacagg gcatgattat aggtacccct gatggaattt
     1921 ttatccacat gagctaactg catttcaatc caggcggaat taaagccttg ttcatgcag
//
LOCUS       DAESHI010000261         1851 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.264, whole genome shotgun sequence.
ACCESSION   DAESHI010000261 DAESHI010000000
VERSION     DAESHI010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.264"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..139)
                     /locus_tag="KW011_004449"
     CDS             complement(<1..139)
                     /locus_tag="KW011_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="activates the csgBA and csgDEFG operons involved in
                     biofilm formation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HBH7662596.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RS"
     gene            892..1347
                     /gene="csgB"
                     /locus_tag="KW011_004450"
     CDS             892..1347
                     /gene="csgB"
                     /locus_tag="KW011_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HBH7662597.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSTQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            1388..1840
                     /gene="csgA"
                     /locus_tag="KW011_004451"
     CDS             1388..1840
                     /gene="csgA"
                     /locus_tag="KW011_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HBH7662598.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT          581 a          341 c          406 g          523 t
ORIGIN      
        1 gagaacgttg aatattatgt aattttcctg taattgccag cgattgttta aggtgctgca
       61 agagagccgt cgcctgcaaa gaaggtttag tgatcaacaa taatgtatga ccatgaatac
      121 tatggacttc attaaacatg atgaaacccc gcttttttta ttgatcgcac acctgacagc
      181 tgcctctaaa atagaagcac cagaagtact gacagatgtt gcactgctgt gtgtagtaat
      241 aaatcagccc taaatgggta aaatataaaa ctaatggatt acatctgatt tcaatctagc
      301 cattacaaat cttaaatcaa gtgttaaaca tgtaactaaa tgcaactcgt tatattaaaa
      361 tgttaatctt aaggttttat taagtttaga aatgatagaa aagttgtaca tttggttttt
      421 attgcacaat tttaaaaaat catacaaatg gtgataactt actaataatg catataaaaa
      481 atatttcggt gtagtccttt cgtcatgtaa aaagttgttg ttttttctcc acacctccgt
      541 ggacaaattt ttactccaaa aagacgaggt ttgtcacggc ttgtgcgcaa gacatatcgc
      601 agcaatcagc gatgggcaag aagaatgact gtctggtgat ttttgatagc ggaaacggag
      661 atttaaaaga aaacaaaata ttttttgcgt agataacagc gtatttacgt ggcttttaat
      721 actttggtat gaacaaaaaa agaaaaatac aacgcgcggg tgagttatta aaaatatttc
      781 cgcagacata ctttccatcg taacgcagcg ttaacaaaat acaggttgcg ttaacaacca
      841 agttgaaatg atttaatttc ttaaatgtac gaccaggtcc agggtgacaa catgaaaaac
      901 aaattgttat ttatgatgtt aacaatactg ggtgcgcctg ggattgcagc cgcagcaggt
      961 tatgatttag ctaattcaga atataacttc gcggtaaatg aattgagtaa gtcttcattt
     1021 aatcaggcag ccataattgg tcaagctggg actaataata gtactcagtt acggcaggga
     1081 ggctcaaaac ttttggcggt tgttgcgcaa gaaggtagta gcaaccgggc aaagattgac
     1141 cagacaggag attataacct tgcatatatt gatcaggcgg gcagtgccaa cgatgccagt
     1201 atttcgcaag gtgcttatgg taatactgcg atgattatcc agaaaggttc tggtaataaa
     1261 gcaaatatta cacagtatgg tactcaaaaa acggcaattg tagtgcagag acagtcgcaa
     1321 atggctattc gcgtgacaca acgttaattt ccattcgact tttaaatcaa tccgatgggg
     1381 gttttacatg aaacttttaa aagtagcagc aattgcagca atcgtattct ccggtagcgc
     1441 tctggcaggt gttgttcctc agtacggcgg cggtaaccat ggtggtggcg gtaataatag
     1501 cggcccaaat tctgagctga acatttacca gtacggtggc ggtaactctg cacttgctct
     1561 gcaaactgat gcccgtaact ctgacttgac tattacccag catggcggcg gtaatggtgc
     1621 agatgttggt cagggctcgg atgacagctc aatcgatctg acccaacgtg gcttcggtaa
     1681 cagcgctact cttgatcagt ggaacggcaa aaattctgaa atgacggtta aacagttcgg
     1741 tggtggcaac ggtgctgcag ttgaccagac tgcatctaac tcctccgtca acgtgactca
     1801 ggttggcttt ggtaacaacg cgaccgctca tcagtactaa tacatcattt g
//
LOCUS       DAESHI010000262         1799 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.265, whole genome shotgun sequence.
ACCESSION   DAESHI010000262 DAESHI010000000
VERSION     DAESHI010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.265"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..1138
                     /locus_tag="KW011_004452"
     CDS             <1..1138
                     /locus_tag="KW011_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBH7662599.1"
                     /translation="TWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAA
                     QLEAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSL
                     RLPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGL
                     KFHVNGDPSDFNQAVEYLKQDSQMKETIDFLSSSEETINIEYIEGTNVRFNSNNMTIY
                     WNSRASLFCSTELNSKSQSPALGLGHEFAHAQYCLLDKENFMALLSRTDKKYENKEEA
                     RVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
     gene            1150..1599
                     /locus_tag="KW011_004453"
     CDS             1150..1599
                     /locus_tag="KW011_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662600.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEIRDLYAGKIIDLLQMKYFRKEKYNNIRWGGSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
BASE COUNT          543 a          297 c          504 g          455 t
ORIGIN      
        1 gacatggtac gggtgggacg gtgacaggct gacgacgata cagaccggca ccacgcgtat
       61 ccagacggta taccagccgg ggagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggtgt
      181 gacgctaccg gcggagctgt cggtgatgct ggggaggctg gagcgggaac tgcgcgcggg
      241 agcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg cggagcagat
      301 ggccgcgcag ctggaggcgg aatacatccc ggagaggaaa cttcatcttt accactgcga
      361 ccaccgggga ctgccgctgg cgctcatcag cccggaaggg gaaacggcgt ggcaggggga
      421 gtatgacgaa tggggaaacc tgctgggcga aaccagcgcg cagcacctcc agcagtcact
      481 ccgtctgccg gggcagcagt atgatgagga gtcggggctg tactacaacc gcaaccggta
      541 ctatgacccg ttgcagggaa ggtatatcac ccaggatccg atagggctga gaggtgaatg
      601 gaatctgtat aaatatccat taaaccctgt tcggtttata gattctttgg gtttaaaatt
      661 ccatgttaac ggtgatccaa gtgattttaa tcaggctgtt gagtatttaa aacaagattc
      721 acaaatgaaa gaaactatag attttttatc aagctctgaa gaaactataa atatagagta
      781 tattgaagga accaatgtta gatttaattc gaataatatg acgatatatt ggaatagtag
      841 agcatcttta ttttgttcaa ctgaattaaa ctcgaaatca caatcgcctg cattaggttt
      901 gggacatgag tttgctcatg cgcagtattg ccttcttgat aaggaaaatt tcatggctct
      961 tttaagccga acagataaga aatatgagaa taaagaagag gcaagggtta ttacaataat
     1021 tgaatcgcgt gcggcgaaaa ctttaggtga gtgtaccaga ggagctcata gtggactgcc
     1081 tttttatcgg gtggatggac cgttgcagac gatgaaaata acgggtacgc cagaatagtg
     1141 aggtttttta tgatgaaata tttgatggtt ttattgtctc tattttctgg ttcggtattg
     1201 gggatggggc gagtgaatga attgtgtgga atagatagcg tgaaaacgat agagataatt
     1261 aatcttccat cgtatgttac cacgttagtt ccgcttagca aggaaggtct taatgaaata
     1321 tacagatata aagtggtggt gaatgaaatt agagatcttt atgctggtaa gataattgat
     1381 ttattgcaaa tgaagtattt cagaaaagaa aaatataaca atataagatg ggggggtagt
     1441 atcatttcga aaggtaataa taaatgtgaa atttattttg atgcttttgg tgaatgtgga
     1501 agtgtgaatg gcattaacgt gtgttttgaa aaaaatgaaa tgataggttg gattaaaaaa
     1561 gaaatccctt tgctgtcgca aaaaataggt gggctttaaa tggtagggca atattcaaag
     1621 acagtatagg taactatttg tatggccatg taaggtaaaa gaaaaggaat aaaccatgag
     1681 cggaaaaccg gcggcgcggc agggcgacat gacccgcagg gaaagatcat cagaagttca
     1741 ctttttgtac taaataattc gcattttatg tttaaaaatt gagatattcc ttattacct
//
LOCUS       DAESHI010000263         1773 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.266, whole genome shotgun sequence.
ACCESSION   DAESHI010000263 DAESHI010000000
VERSION     DAESHI010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.266"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
BASE COUNT          609 a          340 c          272 g          552 t
ORIGIN      
        1 ccgcgatcgg ttttgtgtgt tctgaatacc gaacgggtca aaatgacacg ttttctctcc
       61 ctcaatggag cgttctatac agagattttt gttcccctat ccattattct gattaccggg
      121 tttccaagct gaaaacagga cttttctgcg accagttttt gcgaatatag tcacataaac
      181 caccgattga gtgacagtta ctgaaacaac tatttcgaca caaaatctcg tctattcaac
      241 atcatgcaaa tttgtcagtg actgaattta gtgacagaac cctctggaat tctgccgtgt
      301 aaccacttat atgcagacct gccccgttgc tctgggcttt caggctgcga tgagtgatga
      361 gcgatgattt ccatacagat gatatatcga ctggaattga ttgcctgcaa ttagatttag
      421 ttttattgtg tggatcgttt tagttagtgg ggatgaattt ccgataaatt aaccaatgga
      481 aaggagatga tatgaataaa aagataactg gattattaat aattagtctg gcgtcaattt
      541 tcttaattga ttgcacccag tgccattaac atctatctac acaaaaatag aggaaaacaa
      601 ttttgatata tcaaataatt acaagcagct ccattctttt tattgtttgg gggattacat
      661 ctataataat tagaacaaag aattattttt ctaagaagaa aacagacctt aatataagct
      721 tttttatagc ttccctattt ctataacaat ccttttattt tgccgaggta tattataaat
      781 ataacataat acatctatat ggggtgtaca tatccccaca taaaaacaca tactctcatt
      841 aacacgggaa gaaactatgt tttccaattc tcactacatg tacataatcg ttcttgtact
      901 atggtttagt attctcaagt acttacagag aaagaatagc atattacatc ctttattttc
      961 actaatcctg acaccactaa ccattttaat cattagactt atgatagatt taacatacaa
     1021 ccaataaatt ataaacaaaa taaaaatagg gaacattata tgctttataa aattttcaca
     1081 ttaaacacaa agataaaaac aactatcatt acaatatgtg cattatcata taatcttaca
     1141 gcgcaagctt ccccgtcata cagacctcgt gatcgagtta ataagactat gatagaacta
     1201 caaactaacg gagtagaacg gcaactttta gataattctt gtggcataag ttctggggta
     1261 tttaaataaa atataagaca ggctgtctat cttacagaca gcctttttat aataacagaa
     1321 agaatatata tcatacttaa cggaagagat aatatgaaaa cagtacccgt ataaacagca
     1381 agcaatccca tttttccagg tgtatcagca aaaaaaccgc tgttctagaa atcaggccgg
     1441 gtaaatttca gagctgtatc ttcaatatac cattttgcaa ccggatacaa aaccattccg
     1501 cagagagaaa tacaccagaa cagtaatctg tatttaaaat cataatccca tgacatatac
     1561 agcatatatc ctcccgtcac ccatccccac cacatattat taaaataata ttttctgttc
     1621 atctgccagt tttcctttct tttgcacttt tgtcagtgta ctgatgcatg acaacataac
     1681 cacagcatga taataataat caataacaat aagctgtgtc acgtttacat catggaacgg
     1741 aggtgctcct gtgacttttg agcagaaaaa agc
//
LOCUS       DAESHI010000264         1726 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.267, whole genome shotgun sequence.
ACCESSION   DAESHI010000264 DAESHI010000000
VERSION     DAESHI010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.267"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..354)
                     /locus_tag="KW011_004454"
     CDS             complement(<1..354)
                     /locus_tag="KW011_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HBH7662601.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLA"
     gene            complement(354..848)
                     /locus_tag="KW011_004455"
     CDS             complement(354..848)
                     /locus_tag="KW011_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HBH7662602.1"
                     /translation="MDRELKNLTLNISQLAALSGVHRQTAAARLQNLPVAGGHESNLK
                     LYRVVDIVSAFLALPPPVAEGEMDAHERKAWYQSERERLKFEQETAQLIPASDVRREF
                     AIWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEEL
                     QQEE"
     gene            1206..1382
                     /locus_tag="KW011_004456"
     CDS             1206..1382
                     /locus_tag="KW011_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662603.1"
                     /translation="MRDKPANHKRGKNPVCIVFDYSRTLAQKEFPVGLRSWLIREYGD
                     DTAHDVSGLNTFIR"
     gene            complement(1524..1676)
                     /locus_tag="KW011_004457"
     CDS             complement(1524..1676)
                     /locus_tag="KW011_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662604.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGAHFWSESRLNISLLMLDIEHFA
                     RGKGLR"
     gene            complement(1664..>1726)
                     /locus_tag="KW011_004458"
     CDS             complement(1664..>1726)
                     /locus_tag="KW011_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBH7662605.1"
                     /translation="IRDQTALRTLQEYIRTQCLR"
BASE COUNT          441 a          475 c          399 g          411 t
ORIGIN      
        1 ggcgagtcgt tttttggagt gttcgcgggc tttttcctcc gtcatctgaa taatcagcat
       61 atcagcagga tcgcaaatca cgttgtaaat cacccagccg tcaatcaggc cgatagtctt
      121 gccggttcgt gccgggccaa caaatatcac tgcgtcgtat tcacgcgagg ccaggcagtt
      181 catcggctca ataacatacg gtgccaccag cggatcccac gggactgagt tccctgcccc
      241 catgggcacc cgcatatact gagcaacggc atcagcaacc cgcattcgtc tcggtgcgcg
      301 aaggatataa cctgaatcgg ttcgtgctgc ctttgcggtt tcctgattca gcattactcc
      361 tcctgctgta attcctcctc atcatccgca cctgcttcag tcacccgcag ggctatctga
      421 tcgcgcagat catcaataat ggactgaaca cggctcacag cggcaggctg cagaccgcag
      481 tcacgttcaa gaatatccgg taatgtctcc agcacctgca cgaccgcttt tgcccagatg
      541 gcaaactccc gtctgacatc actggccgga atgagttgtg ccgtttcctg ttcgaactta
      601 agacgctcac gttcagactg ataccaggct ttgcgctcat gcgcgtccat ttcgccttct
      661 gcaaccggcg gtggtaatgc cagaaatgcc gacacaatat caaccacccg ataaagcttg
      721 aggttgcttt catgcccccc tgcaacgggt agattttgca gccttgccgc agcagtctgg
      781 cgatgtacac ctgacagtgc cgccagttga ctgatattca gcgtcagatt ttttaactct
      841 cgatccatac ccgctccaga atgttttaaa catgcatctt gcgaacaact ttaggcaaac
      901 ggtgttagtg atgaacaaaa aacaatcaaa atcgacacca taaaaataaa accactgtaa
      961 tatcaatcca ttacagtagt ggtgatgacg aatgaaattt caaaaactag cctttttccg
     1021 cgccgctccc gccccgtggc aggccacccc accgggagga cccgtcagcc tgacagccat
     1081 gacgaacgtc tgatacagcg ccctgcatga atgacatcgg gataatccag aaaggaatag
     1141 catcgtaccc acaagaatca gtgtaagtgt cctgtttctt ccacccccgc acaggactgg
     1201 cgagcatgag ggacaaaccc gcgaaccata aacgcggtaa aaacccggtg tgcatcgttt
     1261 ttgattattc ccgcacactc gcgcagaagg agttccccgt cgggctacgg tcatggttaa
     1321 tacgggaata cggcgacgat acagcgcatg atgtgtcagg cttgaatacc tttatccgtt
     1381 aaaagggata tcagttaagt tatcccgtgt agggtataag ccattatcaa agccactctg
     1441 taggaagtgg cttttgtaat ggcaataaaa agccccgcga atgcgaggct aaatcctggt
     1501 atttgtaatg actggctctt atctcaacgc agccccttac cgcgcgcaaa atgctcaata
     1561 tcaagcatca gcaatgagat gtttaatctg gattcactcc agaagtgagc accaccctgt
     1621 ctacagagcc agatgtgaag gatgatgagt aaaattatcg ctatcatcga aggcattgcg
     1681 tcctgatgta ttcctgaagc gttctcagtg ctgtttggtc gcggat
//
LOCUS       DAESHI010000265         1687 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.268, whole genome shotgun sequence.
ACCESSION   DAESHI010000265 DAESHI010000000
VERSION     DAESHI010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.268"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(784..1455)
                     /gene="queE"
                     /locus_tag="KW011_004459"
     CDS             complement(784..1455)
                     /gene="queE"
                     /locus_tag="KW011_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HBH7662606.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            1594..>1687
                     /locus_tag="KW011_004460"
     CDS             1594..>1687
                     /locus_tag="KW011_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662607.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDN"
BASE COUNT          551 a          343 c          302 g          491 t
ORIGIN      
        1 atcgcactct atcaattttc acttaaagta tgaactggat atctttattc ataagaacta
       61 ttcatcaaca gcgtatagag gcggttatgt aaaaccactc gttagcctca aaacaaagac
      121 aaatttccag atttattact taatcaatga ataacaagct taaaaaacaa actattaacc
      181 taaagaaaaa attaacagac attaattcaa tattaaaaaa ataatagatt aaaatttctt
      241 aacgatttaa gaatcataca aataacactt tgattaaatt taattttttg tatcgataat
      301 tgtgaacctc tctggcatgg agaactattt tgaacatgag gtgttacgtg gatattttgc
      361 ttattacaag tactgctaat ataaaaactt gagaaagaga taacgggtta tatggtggtt
      421 tatccccgct ggcgcgggga acactctaaa cataacctat tattaattaa tgatttttta
      481 agccagtcac aatctaccaa ctttacagta tcacacaaac aacacatcca ttatgttaaa
      541 gagtacttaa ttcattgatt aaaaaggtaa atatttaaaa taactctata caaattaaaa
      601 tctaccaaac tttaccgcaa taattttcac tccagcgaaa aattaatgcc acagaatttg
      661 tagagaatgc taatgattag ccctggatgg gtaaaatgga ggagttttca gaggcgacaa
      721 acaatataaa tgagtagaag tctttacagg ttccacccat ttataacgct tataaatgtt
      781 taatcaggca atatttagat atttatgtgt ttgcatcgac aaacgccaat tacgcgcaat
      841 gcaggtttca atgcacaaac gtgtggcatc atccttttgg ctaatcggct gcagtgcaat
      901 gactcgcggt ttatcatcgg tcagcgttgc caatagttca tccagtgctt caatatcgcg
      961 tacgcgcccc actggatgct tgatttcgtt ggctcgctcc agtgcctgtg acaacacgtc
     1021 atagccgccg cgcatgttca gctttggcga tacggtaacc caggtattcg gtgtgcagcg
     1081 tacctcatga gtaccactgg tttcgatctg gcagctaaaa ccgttctttt cgagcagatc
     1141 agtcagtggt agcaaatcat gaatgcaagg ctcaccaccc gtaatcacca catgccgcgc
     1201 ggtgtatccc tggcgactaa tgacagccaa caagtcttca ctgctcgccg ccccccactt
     1261 atcactctct ttggtcttcg ccagaatgct gaaaagggag acttcccgat cctcaagctt
     1321 ttcccaggtg tgtttggtgt cgcaccaggc acagccaacc gggcatccct gtaaacgaat
     1381 aaaaatggcg gggacgccgg taaagtaacc ctcaccttgc agggtctgga acatctcgtt
     1441 aatcgggtac tgcatagcat tctctgtgaa gtggataatt gttaattatt gcagatcctg
     1501 ccacaacaat catgtcttat taacattctg ttacaggcag atttaaagaa aaatgcgaaa
     1561 catatcgtta ataattaaag ggagtaacgt attatgtcag aagaaaataa agaaaatgga
     1621 tttaatcatg tcaaaacatt caccaaaatt atatttattt tttctgtatt agtttttaat
     1681 gataacg
//
LOCUS       DAESHI010000266         1629 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.269, whole genome shotgun sequence.
ACCESSION   DAESHI010000266 DAESHI010000000
VERSION     DAESHI010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.269"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            104..292
                     /locus_tag="KW011_004461"
     CDS             104..292
                     /locus_tag="KW011_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HBH7662608.1"
                     /translation="METLLPNVNTTEGCFEIGVTISNTVFTEDAINKRKHERESLNKI
                     CIVSMLARLRLMQKGCWR"
     gene            289..480
                     /locus_tag="KW011_004462"
     CDS             289..480
                     /locus_tag="KW011_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HBH7662609.1"
                     /translation="MNTAFALVLTVILNTGEPAELVTGVYNSMKECVAAAAEQKIPGN
                     CYPVDKIIHQDNKEVPAGL"
     gene            574..>1629
                     /locus_tag="KW011_004463"
     CDS             574..>1629
                     /locus_tag="KW011_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBH7662610.1"
                     /translation="MSKVFICAAIPDEKATKEEGAKPVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESEPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMCPEIKLHAIGWIKHKCKPGAKWPEIQAEMRIWKKCREGERKETGKYTSVVDLARA
                     RANQQHTENSTGKISPAIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGTNQSATD"
BASE COUNT          498 a          361 c          391 g          379 t
ORIGIN      
        1 cggttttatc tggaagaaca gggatggact cttgttaacc ggggcgcaat taaaaatatg
       61 actgtagagc aattactcaa aattcattgt tgaggacgcg ataatggaaa cgttattacc
      121 aaacgttaat acgactgaag gttgttttga aattggtgtc actatcagta acacggtatt
      181 tactgaagat gccattaaca agagaaaaca cgaacgggag tcattaaata aaatatgcat
      241 tgtttcaatg ctggctcgtt tgcgtctgat gcaaaaagga tgctggcgat gaatacagca
      301 tttgcactcg ttctgaccgt tattcttaat acgggcgaac cagctgaact ggttaccgga
      361 gtatacaact ccatgaaaga atgcgtggct gctgcagctg aacagaaaat tcccggtaac
      421 tgttatccgg ttgataaaat cattcaccag gataataaag aagtcccggc aggtctttaa
      481 agcagccacg taataaatat ccggtttcat ttttatatgc cagcaatggc agggatttgt
      541 tcacccttaa atctgtaatg aggttaaaac aaaatgagta aagtctttat ttgcgccgcc
      601 attccggacg aaaaggcaac aaaagaagaa ggcgctaaac ctgtagccac tgccattgaa
      661 gccggcgacg aacgccgcgc ccgcgcaaaa tttcactggc aattcctgga acattatccg
      721 gctgctcagg actgcgctta taaatttatt gtctgcgagg ataaacctgg tataccccgc
      781 cctgccctcg attcctggga tgctgaatat atgcaggaaa accgctggga tgaggagtct
      841 gcttcctttg tcccggttga gactgaatcc gaaccgatga acgtcacttt tgacaagctg
      901 gcccctgaag tacagaacgc tgtcatggtt aagttcgaca catgtgaaaa catcaccgtt
      961 gatatggtga ttagcgcgca ggaactgttg caggaagaca tggcaacatt cgacggacat
     1021 atcgttgaag cgttgatgaa aatgccagaa gttaacgcca tgtgtccgga gattaaactg
     1081 catgccatcg ggtggattaa gcataaatgt aagcctggtg caaaatggcc agaaattcag
     1141 gcagagatgc gcatctggaa aaaatgtcgc gaaggtgaac gcaaggaaac cggaaaatac
     1201 acgtctgttg ttgatctcgc ccgtgccaga gccaatcaac agcacactga aaattcaaca
     1261 ggaaaaatca gcccggccat tgctgccatt catcgcgaat acaagcagac atggaaaaca
     1321 ctggatgacg aactggccta cgctctctgg cctggtgatg tggatgccgg aaacattgac
     1381 ggcagcatcc atcgctgggc aaaaaatgaa gttatcgaca acgaccgcga agactggaag
     1441 cgtatctcgg catcaatgcg caaacagcct gatgcccttc gctacgaccg ccagactatt
     1501 tttggccttg tccgtgaacg tccgatcgac attcacaaag accctgtggc actgaacaaa
     1561 tacattactg aatacctgac tacaaagggc gtgtttgaag atgaaggaac aaatcagagc
     1621 gcaactgat
//
LOCUS       DAESHI010000267         1575 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.270, whole genome shotgun sequence.
ACCESSION   DAESHI010000267 DAESHI010000000
VERSION     DAESHI010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.270"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            289..1404
                     /locus_tag="KW011_004464"
                     /pseudo
     CDS             289..1404
                     /locus_tag="KW011_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          623 a          286 c          221 g          445 t
ORIGIN      
        1 cacatctaaa acatcacaaa aaaaatcact tcacacacta tcagagaaca taataacttt
       61 ttaaaatgag cgcattttga tagttgttaa cgatttcctt tacgatacta tcactaccct
      121 ttttttatac acaaatatta ccgggtggag ataaaaggga aatcaaaggt aattatatca
      181 ggaagatata acgcattaca tttattgtgt gtagaagaaa atccattcct gttctaatta
      241 tccaatttaa acatcttagc ataaaacaaa tgatgaataa ggaattttat gtcaattgac
      301 tttaccccag gtataataaa tacatatcac ggcgatattt ataactgcac aacaaatacc
      361 gataatgcta aaacgccaga cacaccgaag tggccttgcg ataattggga agaacaacaa
      421 cccattaatt ccacattctc tggagaagga tatatctctg atcaatatga tttagtgcaa
      481 caccaacttc aacaaattaa tgcgtgccac acgaacacta catatacaaa tgcagactac
      541 tcaaaagttg tggctcaact tgtaagtctt attactaaca ttgaaacgat aagttcgaca
      601 caacttacgc aacagacaca atcaatactt aatcagatta ataacattag atatgaaaaa
      661 aacaaaagtg ctgaatgtcg aatcattgtt gtcgctaatc ctaaacccga caaggcaatc
      721 ataactaaaa ttagcgtaga agagggaata cccataacat tcagtgtcca aaccatgttt
      781 tcagatacta attttattgc tgaacaacga gctgacttac caactaatat aaaagatatt
      841 caaagcctgt atcaaaaaat gacgaagtta tatattgagc acagtgaaaa taaaaacagg
      901 atgaaagtct ttgccggagc caactttatt gatttcaata tgacaggaca gaacctgtcc
      961 gggttcgttc taaccttgtc aaggttttat ttcgaagatc tacttaatat aaatttcact
     1021 gacgcaaatt taggtgacac tattttttac ataaagaaca ccctaccccc aaattatata
     1081 aagatggaca atatcttgac aaacaaatcg aaggtttatt ttcaacatta ttaaccatta
     1141 atgacaactt tttacgagca aaagcggaaa ttgcttcgac aataattaaa tttttagagg
     1201 ccaggataac caatctttca tacaatgaca tattaaaata tcagcaagaa ttccaaaaac
     1261 aatgttataa gcaagttaaa gcctttacga cactctctcg atacaataaa attcaaacat
     1321 gggcagaaat gtctgaatat cagttcgaag tatttcaata cgagacgctt aaccccaaaa
     1381 aaatgtcaca cacgccttat ttaaagaggc cacttcctaa tgaaaaagat ataaattatg
     1441 gggtggaaat agaaatacca tctggcaaaa gaattcgctt atcaaatcat tatcagaata
     1501 taataccata atattgatta ttagcacttt ataattattg aatgaaaata gattttatgt
     1561 aattttaaaa caaag
//
LOCUS       DAESHI010000268         1534 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.271, whole genome shotgun sequence.
ACCESSION   DAESHI010000268 DAESHI010000000
VERSION     DAESHI010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.271"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            1..1436
                     /locus_tag="KW011_004465"
                     /pseudo
     CDS             1..1436
                     /locus_tag="KW011_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
BASE COUNT          439 a          400 c          372 g          323 t
ORIGIN      
        1 acgaagcaaa aaatgacaaa gtaagaaaac gcctcggtat taaaggcggt ttttactggg
       61 ctgaagcgaa aaaattatcc attgccatct cccgtggtgc tgttgcgatt gacgatgctg
      121 gctacgatga agatgacttt aaaaaacctg ttcgcgtcaa tttgcccgtt gttggtgacc
      181 tcccaccaga aggcgtgttt gatacggaat tctgcaaccg ttacgaaaaa ggcggggaag
      241 atggcatcac aatggtattt atcgcatctt ctccctctgt tcaggacaaa ccagccagca
      301 ctgacaatac caacgtcaac ggcgaagaca tgacagaaat tgaggagaac atgctcctgc
      361 cagtttccgg tcaggaactg cccattcgct ggcttgctca acacggcagc gaaaaaccgg
      421 taacgcacgt ttcacgcgac ggactccagg cattacacat tgctcgggct gaagaactac
      481 cggctgttac tgccctggct gtttcccaca aaaccagcct gctcgacccg ctggaaattc
      541 gcgatctcca caaactggtt cgtgacactg acaaagtttt ccctaatcct ggtaattcaa
      601 gcctgggact gataactgct tttttcgaag catacctgaa cgctgactac accgatcgag
      661 gactgctgac aaaagagtgg atgaagggta atcgtgtttc acacatcact cgcacggctt
      721 ccggtgctaa tgctggcggc ggaaacctca ccgatcgcgg cgaaggtttc gtacacgatc
      781 tgacgtcact ggcgcgcgac gtagccactg gcgtactggc ccgttcaatg gatctggaca
      841 tctataacct tcatccggca cacgctaaac gcattgagga aattatcgct gaaaataaac
      901 cgcccttttc tgttttccgc gacaaattca tcaccatgcc tggcgggctg gattattccc
      961 gcgccatcgt ggttgcgtcc gtaaaagaag caccaattgg gatcgaggtc atccccgcgc
     1021 acgtcactga atatctgaac aaggtactga ctgaaaccga tcatgccaac cctgatccgg
     1081 aaatcgtgga tattgcctgc ggtcgctcct ctgccccgat gccgcagcgt gtaacagaag
     1141 aaggaaaaca ggatgatgaa gaaaaaccgc aaccatcttg cgcaatggca gatgaacagg
     1201 caacggctga aacagtggaa ccggatgcaa ctgaacatca tcaggacacg cagccgctgg
     1261 atgctcagtc acaggtaaat gctgttgatg cgaaatatca gaaactgcgg acagaactcc
     1321 atgaagcccg gaaaaacatt ccgcccaaaa atcctgtcga tgcagacaaa ttactggctg
     1381 cctctcgcgg agaatttgtt gaagggatta gcgacccgaa tgattcgaaa tggattcccg
     1441 gtcatcatat ctcctcaaat gaagtcaaaa aaacggaaaa taaagtgcga cagactgaag
     1501 ataaacagca ccagaacagc gaaccggagg agcg
//
LOCUS       DAESHI010000269         1525 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.272, whole genome shotgun sequence.
ACCESSION   DAESHI010000269 DAESHI010000000
VERSION     DAESHI010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.272"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            100..270
                     /locus_tag="KW011_004466"
     CDS             100..270
                     /locus_tag="KW011_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662611.1"
                     /translation="MVRLPPVVMSAKSATARTTIAREDAEDHQVKLSAQKLEELLASM
                     VKDEVDRNDGIY"
     gene            complement(397..954)
                     /locus_tag="KW011_004467"
     CDS             complement(397..954)
                     /locus_tag="KW011_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBH7662612.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(951..1226)
                     /locus_tag="KW011_004468"
     CDS             complement(951..1226)
                     /locus_tag="KW011_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HBH7662613.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            1412..1525
                     /locus_tag="KW011_004469"
                     /pseudo
     CDS             1412..1525
                     /locus_tag="KW011_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="receptor for colicin S4; incomplete; partial in the
                     middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
BASE COUNT          424 a          324 c          367 g          410 t
ORIGIN      
        1 cgcagacacg tcgtatgcag gaacgtgctg tggttagttt gtgagctttc gatagtggtt
       61 gttatttttg cccttatttg ttccggaggc catggttcaa tggtccgtct gccccctgtg
      121 gtgatgtcag caaaatcagc cactgcgcga accacaatag cccgggaaga tgctgaagat
      181 caccaggtaa agctgtcagc gcagaaactg gaagaactgc tcgcatcaat ggttaaggat
      241 gaggttgatc gcaatgatgg gatttattga cgtcagcgag agcagaagga agaactgaat
      301 aacctgaatg atttacgctc aatcagagcg atggcgatta gcggcaatca cgctcgataa
      361 aattaacctc ggtgatcatc cggggttttg cattcattaa aaccgcatca acctttccac
      421 caattgttct ttacgggcaa cgatccaccc atgttgctcc agataaaatt taaaccgttc
      481 cagagtgcat accatcgcat cggcggggac tttttctgtg aactcgacct gaccgtgttt
      541 atcgaagtgg atcagtaatg cgcatccatt atttggggcg ggggagtttt gtgctgcagg
      601 aggacgtttg tggagttctt cttccattgc gttaaatttt tcaatgtaag cttcttttag
      661 tctggctgct tttttgccag taaatcccat tacgaggaac atgaagccgt cttttgtgat
      721 caggtagcat cgtgattcac gttctgcgcc gttgccaatt tcgacagtct gaacatcggc
      781 cgaaaagttg gctgatgtaa attcgggaga gcagtcgagt gtgtcaatct tagctaaaac
      841 gtgtttatgt tgtttatcga agtagtcggc aacagcttga gaggtggtaa ccaggcgacc
      901 atctttgaaa aagacttttg gtgactcgtt agaaaaagtt atagctgtat tcatatgtga
      961 tgactccaat taattggatg atcaccacct ctgacgccaa tcagggaggt ggtgagacgt
     1021 acagggttgg cgtaaccggt catcacaaaa cccggcgcgg atttctccgc ccctgcacgc
     1081 cccaccataa tgcgaatgtg gcaatgctta acgcaaaaaa aaccgcaaac gcggctatgc
     1141 gttgtgatgt tatccgagac gccaatcccg acaaccgatt ttgcggttgt gtgaggaata
     1201 tagccccgga gaacaaaaac tgtcaacggg caaaaaaatt tccgttatca ggaatgtata
     1261 aaattaaagc gatcgagcaa aaaatccttt ctgcaaaact aaaaaagatt tcaataaaat
     1321 caggcagtta aaaacacgga aaaacggtat aaaaaagcgc ctcaaaatcc tgttttttct
     1381 ttcagataca atgtcttatt gcatttttta aaacatgtca gtgtggtaca tggatatcga
     1441 taccatcgcc aattataaac tgggcggtgc acagcaacat gatagcgtac gcctcgatcc
     1501 gtgggtgttt atgttctcag cagga
//
LOCUS       DAESHI010000270         1482 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.273, whole genome shotgun sequence.
ACCESSION   DAESHI010000270 DAESHI010000000
VERSION     DAESHI010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.273"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..1012)
                     /gene="cadA"
                     /locus_tag="KW011_004470"
     CDS             complement(<1..1012)
                     /gene="cadA"
                     /locus_tag="KW011_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HBH7662614.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYT"
     gene            complement(1092..1439)
                     /locus_tag="KW011_004471"
     CDS             complement(1092..1439)
                     /locus_tag="KW011_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HBH7662615.1"
                     /translation="MTALMILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDL
                     IRFEGVNIRNFVSLICSVLGCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQ
                     SHSMDNHTASNAH"
BASE COUNT          413 a          324 c          371 g          374 t
ORIGIN      
        1 tggtgtaagg cacccacgcg gagtcaaagt ggatggattt cacatccagt gttttcttga
       61 tgaagtcggt gttgtacagc agaccatcat aggtagagtt ggtaattaca gcatgtaccg
      121 gccaggttgc gtttggtgtt tctttcacgc gcttagcaat ggtagcgtgc tggaattcac
      181 tctgtgggat accaccaaga ataccgtaag cgttacgggt cgggcggaaa tagattggcg
      241 taacatcgct catcatcatc aggtgggtca gcgatttgtg gcagttacgg tcaatcagaa
      301 tggtgctgcc tgccggagca gagtacatgc caacaatttt gttcgcagtg gaagtaccgt
      361 tggtcaccat gtagctgcgg tctgcgttaa agacgcgagc gatatactgt tctgcttctt
      421 tgtgtggacc actatgatcc agcagagaac ccagttcaga tactgaaatg gaaatatcag
      481 atttcatggt attcggacca aagaaatcat agaacaggct acctaccggg cttttctgga
      541 atgcagtacc gcccatgtga ccaggagtac agaaagtata tttaccttca cgaacatatt
      601 taaacagtgc tttagtcagc ggaggcagaa tagtgttgat atattcgtca gtggtctgct
      661 tgatcttatt agcaatatct tcagcagcac ccagcgcata ttcaaagaag ctaatctgta
      721 aacgcaggtc attcaggctt acatcgagag tggaatacgt attagcgaac gcgtacaacg
      781 gcaggttctc gttcattttg ctaatttctt cgcacagctc gagattatat ttatcccagt
      841 caaaaataac gccgcacaga cgcgcattgt tttcgatcag ttttaataag tcgtcacggt
      901 cgttcgggta aacaatctgg aagttcagac gttcaagcgc gcgatgaagt tcacggatgg
      961 gttcttcttt aaaataaacc cccatgtgat tcaatattgc aataacgttc atagtcatat
     1021 ctccaggtaa aaaaggcccc tcccaacaca tgggacaaaa tgaaaggagg agcctcggaa
     1081 aatactttta attaatgtgc gttagacgcg gtgtggttat ccattgagtg gctctggcgc
     1141 tcgtgcattt tgcgagcgta gaacatcagg ataatcaggc tgacgatgaa ggtacctgcc
     1201 agctcgaagg agcttgcgcc catcagcgcg atgaagcaga acacacaacc cagtacagag
     1261 cagatcaggc tgacaaagtt gcggatgtta acgccttcaa aacgaatcag gtcaacgcaa
     1321 gagtagaagt acggcagcat agtcagcagt actgcgatac cggtcagttc accgaacagg
     1381 tcagatgctt taccaccggc agagttcatc agagtgatga ggatcatcag ggcagtcatt
     1441 ttcactgcag ccagcagcag taatgccgat cagttaagga tc
//
LOCUS       DAESHI010000271         1476 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.274, whole genome shotgun sequence.
ACCESSION   DAESHI010000271 DAESHI010000000
VERSION     DAESHI010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.274"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..>1476
                     /gene="rhsC"
                     /locus_tag="KW011_004472"
     CDS             <1..>1476
                     /gene="rhsC"
                     /locus_tag="KW011_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HBH7662616.1"
                     /translation="LVLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIR
                     LSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVA
                     HRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAG
                     LKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRAS
                     AFYYNHHSQLTSATGPDGLEMRRKYDESGRLIQETAPDGDIIRYRYDNPHSDLPCATE
                     DATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQ
                     LIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYD
                     AAGRVIRLTSENGSHTTFRYDVLDLLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTH
                     WHYDEADRLTHRTVNGETAERWQYDERGWLTDISHLSEGHRV"
BASE COUNT          351 a          438 c          455 g          232 t
ORIGIN      
        1 ctggtactga ccacgcaggc gcagcgggca gaagaagccc ggcagcaggc catttccggc
       61 gggacggaac cgtccgcttt tcctgatacc ctgccgggtt acaccgaata tggccgggac
      121 aacggcatcc gtctgtctgc cgtgtggctg acgcacgacc cggaataccc ggagaattta
      181 cctgccgcgc cgctggtgcg ctatggctgg acgccgcgcg gcgaactggc ggcagtgtat
      241 gaccgcagca atacacaggt gcgcagcttt acttacgatg ataaataccg gggccggatg
      301 gtggcgcacc gtcacacggg ccggccggaa atccgttacc gttacgacag cgacgggcgg
      361 gtgacagaac agctaaaccc ggcaggctta agctacacgt atcagtatga gaaggaccgc
      421 atcaccatca ccgacagcct gaaccgccgt gaagtgctgc acacgcaggg tgaagccggg
      481 ctgaagcggg tggtgaaaaa ggaacacgcg gacggcagcg tcacgcagag tcagtttgat
      541 gcggtgggca ggctcagggc acagacggat gccgcaggcc ggacgactga gtacagcccg
      601 gatgtggtga cgggactcat cacgcgcatc accacgccgg atggcagggc atcggcgttt
      661 tactataacc accacagcca gttaacgtca gccaccgggc ctgacgggct ggaaatgcgc
      721 cgcaaatatg atgaatccgg ccgtctgatt caggaaactg cccctgacgg cgatatcatc
      781 cgctaccgtt atgataatcc acacagtgac ttaccctgcg caacggaaga tgccaccggc
      841 agccggaaaa ccatgacgtg gagccgttac ggtcagttgc tgagcttcac cgactgttcc
      901 ggttatgtaa cccgttatga ccatgaccgc ttcgggcaga tgacggcggt gcaccgcgag
      961 gaagggctga gtcagtaccg cgcatacgac agccgtggac agttaattgc cgtgaaagac
     1021 acgcagggcc atgaaacgcg gtatgaatac aacgccgccg gtgacctgac caccgtcatt
     1081 gccccggacg gcagcagaaa cgggacacag tacgatgcgt ggggaaaagc tatctgtacc
     1141 acgcagggcg gtctgacgcg cagtatggaa tacgatgctg ccggacgggt catccgcctg
     1201 accagtgaaa acggcagcca caccaccttc cgttacgatg tactcgacct gctgatacag
     1261 gaaaccggct ttgacggccg cacacagcgt tatcaccacg acctgaccgg caaacttatc
     1321 cgcagcgagg atgaggggct ggtcacccac tggcactatg acgaagcaga ccgcctcacg
     1381 caccgcaccg tgaatggcga aaccgcagag cggtggcagt atgacgaacg cggctggctg
     1441 acagacatca gccatctcag cgaagggcac cgggtg
//
LOCUS       DAESHI010000272         1443 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.275, whole genome shotgun sequence.
ACCESSION   DAESHI010000272 DAESHI010000000
VERSION     DAESHI010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.275"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..1117
                     /locus_tag="KW011_004473"
     CDS             <1..1117
                     /locus_tag="KW011_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBH7662617.1"
                     /translation="TWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELAVMLGRLERELRAGAVSAESEAWLAQCGLTVEQMES
                     QMEAEYIPERRLHLYHCDHRGLPQALISPEGETAWRGEYDEWGNQLNEENPHHLYQPY
                     RLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGINLYTYPLAPIRYTDPLGL
                     ERVISVYGPPAPDRAGAETPLVLTDMTGGVTIYYDPETGDSMTFDSSNRIDRRSQRGA
                     GDPYTGEVVGCETNGSGISAAYGTTKIYTTDTRARWLHGGGSSLRDPYAPRQGWKPTM
                     GCTRAQNEDVDELCKKVTSWMYSHPGERIRYERFKTR"
     gene            1095..1334
                     /locus_tag="KW011_004474"
     CDS             1095..1334
                     /locus_tag="KW011_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662618.1"
                     /translation="MNVLRHVNAINMGVCILILVIGCANAKVLKRCILPAPDGGVEKL
                     SKPNLHGVIKKVDLNTNEAEIKIKEGAPIKLSSML"
BASE COUNT          386 a          302 c          439 g          316 t
ORIGIN      
        1 gacctggtac gggtgggacg gtgacaggct gaccactgta cagacccaac agacaagaat
       61 ccagacggta taccagccgg gaagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggggt
      181 gacgcttccg gcggagctgg cggtgatgct ggggaggctg gagcgggaac tgcgtgcggg
      241 tgcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg tggagcagat
      301 ggaaagccag atggaggcgg aatacatccc ggagcggagg ctgcatcttt accactgtga
      361 ccaccgggga ctgccgcagg cgctcatcag cccggaaggg gaaacggcgt ggcgcgggga
      421 gtatgacgag tgggggaacc agcttaatga ggagaacccg catcacctgt accagccgta
      481 ccgtttgccg gggcagcagt atgatgagga gtcgggactg tattacaacc gtcaccggta
      541 ctacgatccg ttgcaggggc ggtatatcac ccaggatccg atagggttga aggggggaat
      601 aaatctatac acgtatccac tggcaccaat acgttacact gatccactgg ggttagaacg
      661 agttataagt gtatatgggc ctcccgctcc agaccgggca ggtgctgaaa ctcctcttgt
      721 tcttactgat atgactggag gagtgactat ttattatgat cctgaaacag gtgattcaat
      781 gacttttgat agtagcaata gaattgatcg tcgttctcaa cgtggtgctg gtgatccata
      841 tacaggtgaa gtcgttggtt gtgagacaaa tggatcagga atatcggcag catatggcac
      901 aacgaagatc tatactacag atactagagc aagatggcta catggtgggg gatcatcgtt
      961 gcgcgatcct tatgctcctc gacaaggctg gaaaccaact atgggatgta cccgtgcaca
     1021 gaatgaagat gtagatgagt tgtgtaagaa agtgacaagt tggatgtatt cgcatcctgg
     1081 agagagaatt agatatgaac gttttaagac acgttaatgc gattaacatg ggagtgtgca
     1141 ttctaatttt ggttattgga tgtgcaaatg ctaaagttct taaacgatgt atattacccg
     1201 ctcctgatgg tggcgtagaa aaactatcaa aacccaactt acatggagta attaaaaaag
     1261 ttgatttgaa cacaaatgaa gcggaaatta aaataaaaga gggtgcccct attaagttgt
     1321 caagcatgtt atgacccctg cggggtataa aaagtcccgt cgcgcatcat ggcgaacaga
     1381 acgtcgcagc gtcgtctcgc cagggcgata agcgcctgat tgtgtcgttt tccctgactc
     1441 att
//
LOCUS       DAESHI010000273         1428 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.276, whole genome shotgun sequence.
ACCESSION   DAESHI010000273 DAESHI010000000
VERSION     DAESHI010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.276"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..118)
                     /locus_tag="KW011_004475"
     CDS             complement(<1..118)
                     /locus_tag="KW011_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HBH7662619.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQH"
     gene            complement(130..822)
                     /gene="rutB"
                     /locus_tag="KW011_004476"
     CDS             complement(130..822)
                     /gene="rutB"
                     /locus_tag="KW011_004476"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HBH7662620.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(822..>1428)
                     /locus_tag="KW011_004477"
     CDS             complement(822..>1428)
                     /locus_tag="KW011_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HBH7662621.1"
                     /translation="KGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFC
                     FGKGVNTPTAFAPTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGAD
                     EEALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVP
                     GAEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA"
BASE COUNT          324 a          397 c          396 g          311 t
ORIGIN      
        1 tatgttgatc aaaagccagc gtaccggaga catacaccac gccatcagcc agcgtgccgg
       61 gaacgaaggg ggccagcggt gcgctgctgc cagcgggaat aattacggat tttggcatcg
      121 ttaaactcct taagcgatac gagcaaagga cgtgggagaa agcgcgtcgc agaacgtttc
      181 gacgtcgctg acccagccaa aaaaggtttc gatattgaac aacgcggctt tctgtgcaaa
      241 ttcaggcccc gcctggtgtg ttgcgtcttc cagcaccacg ccgaaatact ccagaaaaaa
      301 gccatcgcgt agcgtcgatt cgacgcagac gttggtagcg ataccggtga aaaccagatg
      361 gcgtattcca cggctgcgca aaatgctgtc cagcggcgta ttgaagaaac cgctgtagcg
      421 cggcttcggc agcacaatat cgccaggctg cggcaccagt tcatccacca gttgataatc
      481 ccaggagcct ttcgccagca atttcccctg cagctgcggc tgcttacgca tggttttcag
      541 ggcgttcgat ttatgaaaat tcggtgagcc gggtccgcca gcttcgacat actgttcatc
      601 ccagccattt tgaaaccaga tgatcagcat ccctgccgct cgcgctgcgg tcacggcggt
      661 ttgaatgttg gcaatgaccg ggcgagtggt tgagacatca aacccggcga gatctaagta
      721 gccgcctggc gtggcataag cgttttgcat atccaccacg atcagcgcac tttgctgcgg
      781 atcgaaggta atggcttccg gtcgagcggt taaggtcgtc atcatgccac ctcctgagtc
      841 agcgcaggga gatgggcgcg gcactgcatc agtggttgaa tgcgctcgcc gaaggtttcg
      901 attcccgaca gaaaatcatc gaaggtcaac agcacgcctt cggcaccagg cacgcttgcg
      961 acttcatcta acatgcgcgc gacactggcg taagaaccga ctaatgtccc catattgatg
     1021 tttaccgccg aagtgggatc ggccatctga cggacgttgg tgtcagtacc ggagcgggta
     1081 tctttctgac tttgttcggt tagccagctt aacgcctctt catccgcgcc cgctttgtag
     1141 tgttcccatt tggcgcgagc ggcatcgtcg gtttcatcgg caatcaccat aaacaacaca
     1201 taagagccaa cgtcgcgccc ggtttgctct gcggcctgtt tcatccgcac agcggtcggg
     1261 gcgaaagccg tgggtgtatt tacgcctttg ccgaaacaga agttgaaatc ggcatactga
     1321 gcggagaacg ccatgccagc gtcgctttgc ccggcgcaga tcactttcat ggggacactc
     1381 ggttgcggac tgacgcgaca atcattcatg gtgaaaaaat cgccttta
//
LOCUS       DAESHI010000274         1394 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.277, whole genome shotgun sequence.
ACCESSION   DAESHI010000274 DAESHI010000000
VERSION     DAESHI010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.277"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..459
                     /locus_tag="KW011_004478"
     CDS             <1..459
                     /locus_tag="KW011_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH7662622.1"
                     /translation="LNAMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEAT
                     DYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRD
                     GLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            477..1115
                     /locus_tag="KW011_004479"
     CDS             477..1115
                     /locus_tag="KW011_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBH7662623.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
BASE COUNT          444 a          282 c          308 g          360 t
ORIGIN      
        1 ttgaatgcaa tggcgtcgca acgaaccaaa gaggctcgtg gtggagaatt acagatcaac
       61 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      121 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      181 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      241 ttactgactc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      301 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttca
      361 ttacccgatt tctcacatta tgaactgtat gacaaaccgg aagcagttca ggaggcgctg
      421 gaaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      481 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      541 cagataaaca aaccattaaa caaactttat ttgcccgtca gccagcaaaa atccacaaac
      601 cctatccaac taatagcaag attattatgg gagatgtact gaatcatgca gcactgaagc
      661 aggccatgca gggacaggac gttgtgtatg ccaaccttac gggggaagat ttagatattc
      721 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      781 tttccctcgg tatttatgat gaagttccag gaaaatttgg cgaatggaat aatgcagtta
      841 ttggtgagcc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
      901 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
      961 cctcacgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1021 tcacagatat catcgataaa cctgagaaac acattggtga gaatataggt atcaatcaac
     1081 ccgtgactga tggtgacaag ccattattca tgtaaaccaa ttaaatagtt accatcgtaa
     1141 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tccgatgcga
     1201 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacttaca
     1261 tctaagtcag cataattcta atatctgcac atcaataaaa atgcaaacaa cttccggcaa
     1321 aaaaagtgat acaagatcga tgacatagcg ttgaagaaaa ggatttgata atccgcaaaa
     1381 tttgcacaac actg
//
LOCUS       DAESHI010000275         1384 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.278, whole genome shotgun sequence.
ACCESSION   DAESHI010000275 DAESHI010000000
VERSION     DAESHI010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.278"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..1339)
                     /locus_tag="KW011_004480"
     CDS             complement(<1..1339)
                     /locus_tag="KW011_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBH7662624.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNAWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGN
                     EVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSR
                     LTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLM
                     TLQGASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          281 a          391 c          357 g          355 t
ORIGIN      
        1 ctgactggct ctttttcggg gctgtggggt atttccaggg cctctccttt accactctcg
       61 ggaaggccct ttcccttctc gtcggtaatt tcacaagttg tcccatactt gcaagatcgc
      121 gcatcagctc cggtatacgt cccggtgaag cgccctgcaa tgtcatcagc attctcatca
      181 ccattccgca tgattctgag aaactcagtt gattcggcca gtaacctttc agatgttccg
      241 ccattttaat catctgatat ctcaccagat tataagccag taagacaccc cacagctctt
      301 gctccacaag ctccggcttt ttacttctca gcgtcagcct gctcagttgc atcgtctgtt
      361 ttatctccct gtatcccagt tcgatttccc agcgatgact gtacagatcc gccatttctc
      421 ctccggggaa gcgcatggcg tccgtcatcg acgtcagcag atggcagact tttcctttgc
      481 gcgtcacggt cagcaggcgg gctgtcactt catttcccag tcccggccac ttttttcgtg
      541 cctgcgggct ggttttcagc ttcaccagat gatcgccttt acccagtttt ctgagctctt
      601 catattgcgc tccctttctg agaggtatca tccagtggcg gtgttctccc gccaggctcc
      661 aggcatttaa cagtcccagt gagtaataac ctttatccat taacgtcaga gtgttatcgc
      721 cggtttgttc tataagttgc tcagcaagct cattttcgct gttcttcatc gtgccgaagg
      781 ctgcagccgt cagcagatgg ctggtcagtt ccatctggca gaccattttg acctgcgggt
      841 agagcgccgg gttcccggca tgtgtctggc gggggaaggc tgcatcgttc tctggtgtat
      901 ccggtgtgcg ccagaacaca ccatcgatgg ccagcagggt caggccgcac cagtgcggat
      961 gcggcgtggc gttatgccag agctgcgctg ttttcgtgaa cacgcggcgg acagcctcac
     1021 ttcccaggcg ctggcgggcc tgaataacgg cactgggggc aacgaagggg cgattgcccg
     1081 gcagcatgat gtccaggcga ttcacaatct ggtgaagagg ttctttacgc tcaagcgcca
     1141 tgccaacaat acaccagacc atcatttcga ggggaagacg gcgcttgcgt agcgttacag
     1201 tacctgattc ggcaaggcaa cgagagatga gttcggggtc gaggtaatcc cccagagaag
     1261 tcagtgggtt acgcagagaa tcgtaacggg ataccagatc aagagcctgt ccaatgtgca
     1321 taaaaaaatc cggaaacaag tgagcgtttc cggattctta cacagccact ggatcggtca
     1381 actg
//
LOCUS       DAESHI010000276         1383 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.279, whole genome shotgun sequence.
ACCESSION   DAESHI010000276 DAESHI010000000
VERSION     DAESHI010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.279"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..181
                     /locus_tag="KW011_004481"
     CDS             <1..181
                     /locus_tag="KW011_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HBH7662625.1"
                     /translation="PQHLFESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYM
                     AETIAHHQVDIDLED"
     gene            187..1284
                     /locus_tag="KW011_004482"
     CDS             187..1284
                     /locus_tag="KW011_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH7662626.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSILIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIEFLNLGGEHLGIDTSRDNSELFANNGTPTWLKEKRA
                     KEIALDEIIELFNKHHGGTSNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNAL
                     WIELEGVEYAFPDPQTQNETQQAGYDENIPV"
     gene            1323..>1383
                     /locus_tag="KW011_004483"
     CDS             1323..>1383
                     /locus_tag="KW011_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HBH7662627.1"
                     /translation="MEVFMYKITATIEKEGGSPA"
BASE COUNT          431 a          307 c          332 g          313 t
ORIGIN      
        1 gcctcagcac ctgtttgaat cgctctgcat cgaccatttg caacgccacg gtgccaataa
       61 acaggcaatc agccacgcat ttgatgatga tgttgagttt caggagcgca tggcagaaca
      121 catccggtac atggctgaaa ccattgccca ccaccaggtt gatattgatt tggaggacta
      181 aaatctatgg cgctttttca acgagcaata aacacacagg cctatctaaa agcaggaatt
      241 atggggtttg ctggcgacgg caaaacctac acagcaagcg aactggctat tggtctggta
      301 atgcttatgc ggcaacgggg aattgaagct ggtaaccgcc cggtaatgtt tctggacaca
      361 gaaacaggct ctgactgggt taaaccccgc tttgatgcgg aaaatatcga attatttacg
      421 gcaaaaaccc gctcgtttgt tgatctgctt gaagccatta atgaagcgga aagcagcggt
      481 tccgtaatga tcatcgactc catcagtcac ttctggaccg gattatgcga cgagtatgcc
      541 agacgccgaa accgtaaacg aggactggaa ttttcagact gggcgtggct caaacaggag
      601 tggcgacgct ttaccgatcg atttgttaac agtcaggcgc acattattat gtgcgggcgt
      661 gcaggatatg agtatgactt tttcgaaggt gatgatggca aacgccagtt ggcaaaaacc
      721 ggtataaaaa tgaaagcaga aacagaaacc ggttatgaac cctccatcct gatccagatg
      781 gagaagcaaa tgaacataga aaccggtcag gtgtggcgca ccgccagaat actaaaagat
      841 cgctccaccc gtattgatgg acaggtattt tcaaacccaa catttaaaaa ctttcttcct
      901 catatcgagt ttcttaacct gggaggtgaa cacctcggca tcgatacctc acgggataac
      961 agtgaattat ttgccaacaa cggtacaccg acatggctaa aagaaaagcg tgcaaaagaa
     1021 atcgcacttg atgaaattat cgagttgttc aacaagcacc atgggggcac cagtaacgac
     1081 gccaaacgcg caaaagctga tttgctggaa aaaacattca cctcccgctc atgggaaaga
     1141 atcaaaggta tggactggcc gacaatcaaa gctggtcgta atgcactatg gattgaactg
     1201 gaaggcgttg aatatgcatt cccggatcca caaacacaaa acgaaacgca acaagcagga
     1261 tacgatgaga atattccggt gtaaaaccaa gtgcattttg gacacggttt ttgaaaatat
     1321 taatggaggt gttcatgtat aaaattacag ccactattga aaaggaaggt ggttctcctg
     1381 cca
//
LOCUS       DAESHI010000277         1377 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.280, whole genome shotgun sequence.
ACCESSION   DAESHI010000277 DAESHI010000000
VERSION     DAESHI010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.280"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            4..984
                     /locus_tag="KW011_004484"
     CDS             4..984
                     /locus_tag="KW011_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBH7662628.1"
                     /translation="MVGVPVPDNIHPMIKDEAGVWSWRTPVLKGNLYEYFFNVDGVRS
                     IDIGTAMTKPQRQVNSSMVLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTPP
                     GYTGMGEPLPVLYFYHSFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETDA
                     KGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQG
                     GYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTVVVGDK
                     DVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            999..>1377
                     /locus_tag="KW011_004485"
     CDS             999..>1377
                     /locus_tag="KW011_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBH7662629.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGL"
BASE COUNT          360 a          315 c          362 g          340 t
ORIGIN      
        1 ttagtggtgg gtgttccggt tccggacaat attcacccga tgatcaaaga cgaagcagga
       61 gtctggtcgt ggcgcacgcc tgtcctgaaa ggcaacctgt atgagtattt tttcaatgtt
      121 gatggtgtac gcagcattga tataggcacc gcaatgacta agcctcagcg ccaggttaac
      181 tccagtatgg ttctggtgcc aggcagttat ctggatacgc gttctgttgc gcatggtgat
      241 ttgatcgcca taacttacca ctccaacgca ttgcaatctg aacgtcagat gtatgtctgg
      301 accccgccag gatacaccgg catgggcgag cctttgccag tgctctattt ctatcacagt
      361 tttggtgata ccggacgttc cgctatcgat caggggcgta tcccgcaaat catggataac
      421 ctgctggctg aagggaaaat taaaccgatg ctggtggtga tcccggatac cgaaaccgat
      481 gcgaagggca ttattcccga agatttcgtg cctcaggaaa gacgtaaagt cttttatccg
      541 ctgaatgcta aagcggcaga tcgcgaactg atgaacgata ttatcccgct gattagcaag
      601 cgttttaatg tccgtaaaga tgccgatggt cgcgcgctgg cagggctttc acaaggcggg
      661 taccaggcgc tggtttccgg aatgaatcat ctggaaagct ttggctggct ggccacattc
      721 agtggtgtta ccacgacaac cgtaccggat gaaggtgtcg cggcccggct gaacgatccg
      781 gcagctatta accagcaact acgtaatttt actgtggtcg ttggtgataa agatgtcgta
      841 accggcaagg atatcgccgg gctgaaaact gagcttgagc agaaaaaaat taagtttgat
      901 taccaggaat atcccggcct gaaccatgaa atggatgtct ggcggcctgc ctatgcagcc
      961 ttcgtacaga aattatttaa ataaaataag gcattaagat gaaattaatc attaccgaag
     1021 attaccagga aatgagccgt gtcgcggcac accatctgct tggttatatg tcgaagacgc
     1081 gtcgtgttaa cctggcaatt accgccggta gcacgcccaa aggcatgtat gaatacctca
     1141 ccaccctggt taaaggtaag ccctggtacg ataactgcta tttctataat ttcgatgaaa
     1201 ttccctttcg cggcaaagag ggagaaggcg taacgattac caatctgcgt aatctgtttt
     1261 tcacccctgc ggggatcaaa gaagagaata tccagaagct cactattgat aactaccgcg
     1321 agcatgatca gaaactggcg cgtgaaggcg gcctggattt ggtggtgctg ggattag
//
LOCUS       DAESHI010000278         1364 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.281, whole genome shotgun sequence.
ACCESSION   DAESHI010000278 DAESHI010000000
VERSION     DAESHI010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.281"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..1259)
                     /locus_tag="KW011_004486"
     CDS             complement(<1..1259)
                     /locus_tag="KW011_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH7662630.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYT"
BASE COUNT          379 a          348 c          305 g          332 t
ORIGIN      
        1 gtgtaaccta ttgcgttgtt tgtattacgg tattcggcaa cgactttaat cattccctcc
       61 atcatacttg ccacttctgt ttcctgcggc gagatcatgc ggacattttt catgacctgg
      121 gattgcatca ccgtctggct accagagtct tccgggcgtt gccaggtctg gatctcctga
      181 tcgttaccgc caacagtacg ccagttggta attgcaccac tgaagatgtc acgcacctgt
      241 tgttcagtca gggaattaac cggattatcc gcattgacga tgaaaacaaa cgcttcacgg
      301 gcaaaaggcg tgtacagcaa agtaacgccc gattcctcag cgcgtttttt ctgcccaccg
      361 gaaggttgcg ccacaaaaat aatatcagca tcacctttaa caatcctgtt atacgcatcg
      421 ggggtacgag agctctccag atattccctg gtgtgaaaat cttccggtat aacgcttaaa
      481 gcataaaatg cagaggcata aattgggtac gccgccgtag caccatcgag tcgcggccag
      541 ttttgcgtaa actgaatttg cggtttacca cgcagtggtg tcagttgatt attgagttta
      601 tcgggtcgcc aggcccaggt atcaatattt tcgcgcacac cgacaccggg attgagctgt
      661 ttatataaat aaccctgata accagcgaca aaaccaagca tgagaaggat aaacaggagg
      721 gaattacggc aacgtagccc ggatgtaccg gttaaaggat gtctggttgg ccggccgtac
      781 cccagcgaaa aactgatatg cgcgccaacg ggtataatga ctatccacca gtactcacct
      841 ccgactactg agactaacaa aacgcccagc caggggagca taagatcagc caaccaaccc
      901 gatcccatct ggtgaaattg ataatctgcc agatgcagcg cgattatcca gccaaccagg
      961 attaataaac cgggtactaa aaaaagacat tgtcgccaga cggctatttc tcttttttct
     1021 ggtgcactat cgcctgccca tactcgcccc gagaaaaata gcattaatgc gcccagcgcc
     1081 agcataaaaa tgttgagcag aaggtaactt gttggcgtga taaggatatt acaaacttca
     1141 ataataccgg caagtccgac acccagcata gcaacaaccg ccgccaaaaa ttgcgtcagt
     1201 atggggatgc cgaaaaaagt cattaccagc gaggacaaaa tccattttct gttttgcatt
     1261 attctttcca ttctttttga atggtgaaat tatactcccc gagtcccctt gccccttctg
     1321 gacacttttc cgaaatgatg gcggaaaaaa acgggaccct ttgg
//
LOCUS       DAESHI010000279         1341 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.282, whole genome shotgun sequence.
ACCESSION   DAESHI010000279 DAESHI010000000
VERSION     DAESHI010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.282"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..184
                     /locus_tag="KW011_004487"
     CDS             <1..184
                     /locus_tag="KW011_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662631.1"
                     /translation="TISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQ
                     GKRRCQQQFTSQEVQN"
     gene            194..>1341
                     /gene="yicI"
                     /locus_tag="KW011_004488"
     CDS             194..>1341
                     /gene="yicI"
                     /locus_tag="KW011_004488"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HBH7662632.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPG"
BASE COUNT          335 a          318 c          351 g          337 t
ORIGIN      
        1 cacgattagc atcattattg cgctattcac gattgttccg gcgatctgtt atttgctgag
       61 cgcgattatc gctaaacgct actactcact cacgacgcac aatctgaaaa ccgttatgga
      121 acagctggct cagggtaaac gccgttgcca gcaacaattc acctctcaag aagtgcagaa
      181 ctaaggaacg gcaatgaaaa ttagcgatgg aaactggttg attcaacctg gcctcaattt
      241 gattcacccg cttcaggtgt tcgaggttga acagcaggat aatgaaatgg tggtctatgc
      301 tgccccccgt gatgtgcgtg aacgtacctg gcagcttgat acgcctttat ttacgctgcg
      361 ctttttctcc ccacaggaag gtattgtcgg tgtgcggatt gagcattttc agggggcgct
      421 gaataacggt cctcattatc cgctcaatat tttgcaggac gtgaaggtca caatcgaaaa
      481 cacagaacgt tatgctgagt ttaaaagtgg caacttaagc gcgcgtgtca gcaaaggtga
      541 gttctggtca ctggattttc tgcgcaacgg cgaacgtatt accggtagtc aggtgaaaaa
      601 taatggctac gtgcaggaca cgaataatca acgcaattat atgtttgagc ggcttgatct
      661 tggcgttggc gaaacagttt acggtctggg agagcgcttt actgccctgg tgcgcaatgg
      721 ccagacggta gagacctgga accgggacgg cggcacaagt actgaacagg cgtataaaaa
      781 tatcccgttc tacatgacta accgtggtta tggggtactg gtcaatcatc cccagtgtgt
      841 ctcttttgaa gtgggatcgg agaaagtctc caaagtgcag ttcagcgttg agagtgaata
      901 tctcgaatac tttgttatcg acggcccgac gccgaaagcg gtacttgatc gttatacccg
      961 ctttactggt cgtccggcgc tgccgcccgc gtggtccttc ggcctgtggc taaccacttc
     1021 atttaccacc aactacgacg aagcgacggt aaacagcttt atcgatggta tggcggaacg
     1081 caatctgccg ctgcatgttt tccactttga ctgtttctgg atgaaagcct tccagtggtg
     1141 cgattttgag tgggacccgc tgactttccc tgacccggaa gggatgatcc gccgcctgaa
     1201 agcgaaaggg ctgaaaatct gcgtctggat taacccctat atcggtcaaa aatcccccgt
     1261 ctttaaagag ttacaagaga aaggctattt actcaaacgc ccggacggtt cgctatggca
     1321 gtgggataaa tggcagccag g
//
LOCUS       DAESHI010000280         1331 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.283, whole genome shotgun sequence.
ACCESSION   DAESHI010000280 DAESHI010000000
VERSION     DAESHI010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.283"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..182)
                     /locus_tag="KW011_004489"
     CDS             complement(<1..182)
                     /locus_tag="KW011_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH7662633.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFTDEAVSGSVK
                     ASLRNGFSRLLAYAREG"
     gene            981..1277
                     /locus_tag="KW011_004490"
     CDS             981..1277
                     /locus_tag="KW011_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HBH7662634.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT          389 a          268 c          275 g          399 t
ORIGIN      
        1 ccttctcgtg cataagctaa tagtctgctg aaaccgtttc ttaaagaagc tttgacagaa
       61 ccactgacag cttcatcagt gaaccattta gcaatgttgt accctgcctc ttcaatttgc
      121 atttgctggt tagtgatgga ttggtcatct gtactcaccc tgcaatatcc aagtatcgcc
      181 ataatgttcc ccttagaagt ctcaaaagtt agtctcataa gtttagctgt ctcataatcc
      241 aaaaacaacc ttttgagact acttgagggt gcattctacc gttacgttga aagtgtctca
      301 aaaggaagtc cttttaagac tggttatgaa ggagctatga ggttgattag gggttggtac
      361 aagcccaact aaccgaatca actatgaaga ttttaggagg gtaactgtaa ccatcacttt
      421 ctaactttca cgtaggaaat tgttaagtgg tttgatagtt acaccatgtg accaagcatc
      481 tatatatcgt tatatcgccc tgtatatagc actatatgca gtgtcgagag aggaacgact
      541 aacacgatac agtttaccct gaaaagagac ttcacagatt atttatatat ttcaataagt
      601 tagggtaaaa agggactata ccgagactgt atagaatctg tgtagaattg catagatact
      661 tggaaatcta ctattaaatt gcatagggac tcacctgata ttgtcccgtt tttaacaggt
      721 ctcctagaaa accgaataga cttcattcaa gggtcattga agtcataatc atttcagtag
      781 aaaaaccaag tctcgatttt aaatgcaaat agcaatcata ttcaataagg caagacgatc
      841 aggatcagtg aaaatgtcat ttttggcgta attgacctta ctttatgcat ggtcttaaca
      901 aagacatcat ctttccgttg cagatcttcg ccctttcaaa taacgtactt tacaactttc
      961 ccgaacaagg agttgtgccc gtgttgtatg ttatttacgc tcaagacaaa gctgatagcc
     1021 tcgaaaaacg cctttccgtt cgtccggcac atttagcacg tttacagtta ctgcatgatg
     1081 aaggtcgttt gttgacagct ggtccaatgc cagcggtaga cagtaacgat ccaggggctg
     1141 cgggatttac tggctcaacc gttattgctg aatttgaatc gctggaagca gctcaggcct
     1201 gggccgatgc tgacccgtac gtagcggcag gtgtctatga acacgtttca gtgaaaccat
     1261 ttaagaaagt gttctgaata gagattcaat atttaagggc acggtttttg caaccgtgtt
     1321 cttgtttggc c
//
LOCUS       DAESHI010000281         1282 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.284, whole genome shotgun sequence.
ACCESSION   DAESHI010000281 DAESHI010000000
VERSION     DAESHI010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.284"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            132..1151
                     /locus_tag="KW011_004491"
     CDS             132..1151
                     /locus_tag="KW011_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HBH7662635.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
BASE COUNT          368 a          285 c          357 g          272 t
ORIGIN      
        1 cacacaatta aacacggagc agtatgatgg ttaaaagggt gaaactggca ggtgtggagc
       61 ctcgcagatt tccggcatca tagtgcccaa tacgggatgc ataaagctcc ctcagactgg
      121 agactccaca aatgaaatat acaccggttg gcgttgatat cgcaaaacat gtcattcaga
      181 ttcacttcat caatgagcac acaggtgaag tggttgataa acagttgcgt agacaggatt
      241 ttctgacgtt cttcggcaac cgtgagccat gcctgattgg tatggaggcc tgtggaggtt
      301 ctcagcactg ggcacgggaa ctgacaaaac ttggtcataa agtccggttg ttgcaggccc
      361 gcttcgttaa ggcattcgtc atgggcaata agaatgatgt gatggatgcc cgggctatct
      421 ggatggcggt tcagcagccg ggtaaagaaa tcgccgtaaa aacagaagaa cagcagtcgg
      481 tactggttct gcaccgtacc cgcatgcaac tggtgaagtt ccggaccgca caaattaatg
      541 ccctgcacgg gacgttactg gagtttggtg aaaccatcca caaaggccgg gcagcgatgg
      601 agcgggagtt ccccgaagca ctggaacgga tgaaagagag actgccaccg tatctcatta
      661 tggttctgga aaaccagtac aaccgactga atgagctgga ctcactgata gaggatattg
      721 aaaaacagct taccagcgtg gcgaggcaga atgaaacctg taagcggttg ctggatattc
      781 ctggcgttgg accacttatt gcgacggcag cggtggccac catgggggaa gcatcagcgt
      841 ttaaatcggg gcgagagttc gccgcatatg ttggtctggt tccaaaacaa actggctccg
      901 gagggaaagt acgtctgctg gggataagca aacgtggtga cacttatctc aggacattat
      961 ttatccacgg tgcaagagcg gtggcattag tagctaaaga gcctggcccg tggataaccg
     1021 aactgaaaaa acgtcgtcca gccagtgtgg caatcgtcgc catggcaaac aagctggcac
     1081 gaacagtatg ggcgataacc gcccatgacc gtaagtatga caggaaccac gtcagtatca
     1141 gaccatatta atcgctgata ccattaaaca atgaactctt aacaaaaggg tgaatgctga
     1201 aaggttgcta tggcggccag agtgatgaca aagacaggta agaccgtgac tcactaaacc
     1261 tgaacagtat tttgggcttg aa
//
LOCUS       DAESHI010000282         1234 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.285, whole genome shotgun sequence.
ACCESSION   DAESHI010000282 DAESHI010000000
VERSION     DAESHI010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.285"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..761)
                     /locus_tag="KW011_004492"
     CDS             complement(<1..761)
                     /locus_tag="KW011_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBH7662636.1"
                     /translation="MLNVAIENQNGWNYSAPAPYKTGAGRGNPNVTRAHSRAEAVFLC
                     VMHSSIQIMVGCAGQSQDWPGSRVTGISTPVRLTTLMVVENLGGELINLSLEDAIMAT
                     IPALSHPDVTIENGRAVTTSIAIAEFFGKRHDNVLRAIANTECSPEFNALNFEDVTYT
                     DAKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPTDKI
                     IPGDGRTLVVHFDERGNIRFTETVPDGARVCTMET"
BASE COUNT          307 a          299 c          310 g          318 t
ORIGIN      
        1 aaagtctcca tggtacagac ccgtgcgcca tcaggaacgg tttcggtgaa tctgatgttg
       61 ccgcgttcgt cgaagtgaac aaccagggtg cggccatcgc ctggaattat tttgtcagtg
      121 ggtggggtgt tattctggcg cagttcggcc tccatgcgat cgaactcagc aatgtaggct
      181 tctttaaatg cagcggcttt tttgccagtg aatcccatca ccaggaaaac gaagccgttt
      241 ttggtgattt ggtacatcgg gcgtttttcg cctttggcgt cagtgtaggt gacgtcctca
      301 aaattgaggg cgttaaattc aggtgagcat tcagtatttg cgatggcacg taacacattg
      361 tcgtgacgct tgccaaagaa ctctgcgatc gcaatagacg tagtgacagc gcgaccattt
      421 tcgatggtta cgtcagggtg agaaagggca gggatagtag ccatgatggc atcctcgagt
      481 gataagttaa ttaactcacc accgaggttt tccacgacca taagggtggt gagacgtaca
      541 ggggtggaaa taccggtcac tcgagaaccc ggccagtctt gcgactgccc tgcacatccc
      601 accataattt gaatggagct gtgcattacg cataaaaaaa ccgcttcagc gcggctatgc
      661 gctcgagtaa cgttcgggtt tccacgcccg gcacccgttt tataaggtgc aggtgcacta
      721 taattccacc cgttctggtt ttcaatagct acattcaaca ttttctcttt cctttcatca
      781 ccgaagtgaa ctttgttgat gcggtgcctg gtgcctccag gtgacgttaa ccagttaaca
      841 attaacgccg gatacaggga aacccataac cacccgatac gggggaacta atcgcttttt
      901 aactgtgccg cgtgcgctga gccgcattca ccgcatcaca aaattcactt taaaaagggc
      961 ggacatcagc cagcaatgaa actgatgccg ccaaaggtac caatcaacat ggagtgttgt
     1021 ggcgtggttg tcacttaagc gtatggtcaa cctgacaacc cggtgtcctc aacggggaag
     1081 gaataactcc gccatactta ccgccgcgtc atttcgcgtt gtgtgcctgc ttttaaccac
     1141 gtcaggcgag gtggtatcct tcttatcccg aataaccaag aaggaaatct atatgactaa
     1201 agaagaattt gtctcttata tttttgataa aacg
//
LOCUS       DAESHI010000283         1229 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.286, whole genome shotgun sequence.
ACCESSION   DAESHI010000283 DAESHI010000000
VERSION     DAESHI010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.286"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..>1229)
                     /locus_tag="KW011_004493"
     CDS             complement(<1..>1229)
                     /locus_tag="KW011_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBH7662637.1"
                     /translation="RNGNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFTD
                     CSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGDL
                     TAIVAPDGSRSEIQYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYDP
                     VDRLTEQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPAEQW
                     QYDDHGWLTEISHLSEGHRVAVHYGYDDKGRLTGERQTVETPETGEMLWEHETGHAYS
                     EQGLATRQEPDGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGEAYE
                     LATAWNTSGQLRSRHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHT
                     TAANLDIDIPYATDPAG"
BASE COUNT          183 a          421 c          346 g          279 t
ORIGIN      
        1 tttcctgccg ggtctgttgc atacgggata tcgatatcca ggttcgctgc cgtggtgtgg
       61 acgcccgtca gcctccccgt gtcgctgtaa cggtactccc ggctctcctg cgggccactg
      121 atgcggatta gctgtccgtt gtcgttccag tcgtagtcac ggtcaagctg cggaaggttc
      181 aggtgcctgc tccggagctg gccgctggta ttccaggcgg tggcaagttc atatgcctcc
      241 ccgccgaagc tgcgggccgt ctcacggtgc agccggtccc gcgtgtactc gaccagcggt
      301 gttccgccca gcttcatccc cgcaagataa ccgctgccat acgtcagcca ctccaccggc
      361 ggcaggccgt ccggctcctg acgggtcgcc agcccctgtt cgctgtacgc gtgccccgtc
      421 tcatgctccc acagcatctc ccccgtctcc ggggtctcca ccgtctgccg ttcgcccgtc
      481 agacggcctt tatcgtcata gccgtagtgg accgccacac ggtggccttc actcaggtgg
      541 ctgatttctg tcagccagcc gtggtcgtca tactgccact gctccgccgg ttcaccgttc
      601 accgtgcgcc gggtgatacg gtccgacgca tcgtaatgcc agagggtgat aagcccctca
      661 tcttcactct gcgtgagttt tccggtcagg tcatactggt aacgttgcgt ccggccgtca
      721 aagccgcgct gctccgtcag cctgtcgacc gggtcatacc ggaacgtgga ctggctgccg
      781 ttctcgttgg tcagcacagt gatgcgcccg gcggcgtcat accccatgct tcgcgtcaga
      841 ccgccctgcg tggtgctgac ggcctttccc cacgcatcat actgtatctc actgcggctg
      901 ccgtccgggg caacgatagc ggtgaggtct cctgcggcgc tgtactcata acgggtttca
      961 cgcccctgcg catccctctg actgaccagt tgaccgcgcg ggttataact gctgtaagtg
     1021 ctgatgcctt cttcccggtg aacggcgatt tgttgaccgt accggtcata ctcataccgc
     1081 gtcgtgtacc ccgagcagtc cgtaaacgcc tgaagctgac cgtaccggtt tcgcgttatt
     1141 ctccgcctca ccccggttcc gtcttccacc gcacagggca ggccactgcg ggaagaatca
     1201 taaaaccagc gcgtaatgtt accgttgcg
//
LOCUS       DAESHI010000284         1211 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.287, whole genome shotgun sequence.
ACCESSION   DAESHI010000284 DAESHI010000000
VERSION     DAESHI010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.287"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..680)
                     /locus_tag="KW011_004494"
     CDS             complement(<1..680)
                     /locus_tag="KW011_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBH7662638.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
     gene            complement(996..>1211)
                     /locus_tag="KW011_004495"
     CDS             complement(996..>1211)
                     /locus_tag="KW011_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBH7662639.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTKVQGTVVMFSASGKK"
BASE COUNT          355 a          211 c          252 g          393 t
ORIGIN      
        1 aacaaagttt gcattattct gggagatagt tgattttctc taagattgac aacgctatca
       61 gaactgaggc caagtatgct ttccggaatt gccgtgatcc ggttctgatt aagccagaat
      121 attcttataa tatgatcatc tctttctgga aaatctggta ttacttccaa agcatttctg
      181 gctgcatcaa gtaattccaa tgacattggt aacctcggaa gtacagacag gtgattgtca
      241 cttacattaa tatattctag tgacgcaggt aattcaggaa gtgcgaacaa atggttatca
      301 ctcacattta tatattccag cgacactggt aattcaggaa gtgcagataa ttgattactg
      361 cttgcattca gctctttcaa tgcccttggt agctcgggga gcattgatag ttggttattg
      421 cttacattaa tttcatcaag attgtcaggc aatcgtggta gagatctgag acctaaacaa
      481 gataagtcca acgatgtttc actgttctcc agacataatt ggagccgggt aaaagcagtt
      541 tccctgtttt ctccggaaat gctgttttta gtccattcaa cccattcgga gagataatta
      601 ttgtgaacat tgtcgattga tgtagttctg taaaaagaga cgtttccagt ggataggggg
      661 ggattattta caggaagcat aataacctcg cagaaggata ttctgataaa atgtgatact
      721 gggaaataat aagctgaaat aaaatgtttc aggattgaaa tttattgttt tcattttaag
      781 gaagagatgt tgatttaact gtaaaaaaac gatactattt tcttagatgg taaatgtctc
      841 gcctgtgcta tattttgttt ttcggttagt taaatatcgt acgttcatat ttgaacgttc
      901 tgccggaatg cattatcaat agaggtaaag tcgcaacccc aaatcgtaaa ggaaaccgta
      961 gcacgtctta agcaagaacg tgctgcggtt ggatgctatt ttttccctga agcggaaaac
     1021 attactacag taccttgaac cttggtttta acattctcga aatgctctga gagtatatgt
     1081 gttaagcctt cttcggaatc ttttgtgttt gaaaagatgc ctttctgatt gtaaatgcgc
     1141 atcagttttt gaccgaagct attgtgcaca actccatcgc caagaattgt ggctccgtat
     1201 agagttccat c
//
LOCUS       DAESHI010000285         1171 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.288, whole genome shotgun sequence.
ACCESSION   DAESHI010000285 DAESHI010000000
VERSION     DAESHI010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.288"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..>1171
                     /gene="fimD"
                     /locus_tag="KW011_004496"
     CDS             <1..>1171
                     /gene="fimD"
                     /locus_tag="KW011_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HBH7662640.1"
                     /translation="SHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHIN
                     TWLERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIA
                     RGTAQVTIKQNGYDIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYS
                     SVPLLQREGHTRYSITAGEYRSGNAQQEKTRFFQSTLLHGLPAGWTIYGGTQLADRYR
                     AFNFGIGKNMGALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGY
                     RYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQL
                     GRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQM"
BASE COUNT          331 a          261 c          283 g          296 t
ORIGIN      
        1 agccattatg catatttaaa cctacagagt gggctaaata ttggtgcgtg gcgtttacgc
       61 gacaatacca cctggagtta taacagtagc gacagttcat caggtagcaa aaataaatgg
      121 cagcatatca atacctggct tgagcgagac ataattccgt tacgttcccg gctgacgctg
      181 ggtgatggtt atacccaggg cgatattttc gatggtatta actttcgcgg cgcacaattg
      241 gcctcagatg acaatatgtt acccgatagc caaagaggat ttgccccggt gatccacggt
      301 attgctcgtg gtactgcaca ggtcactatt aaacaaaatg ggtatgacat ttataatagt
      361 acggtgccgc cggggccttt taccatcaac gatatctatg ccgcaggtaa tagtggtgac
      421 ttgcaggtaa cgattaaaga ggctgacggc agcacgcaga tctttaccgt accctattcg
      481 tcagtcccgc ttttgcaacg tgaagggcat actcgttatt ccattacggc aggagaatac
      541 cgtagtggaa atgcgcagca ggaaaaaacc cgctttttcc agagtacatt actccacggc
      601 cttccggctg gctggacaat atatggtgga acgcaactgg cggatcgtta tcgtgcgttt
      661 aatttcggta tcgggaaaaa catgggggca ctgggcgctc tgtctgtgga tatgacgcag
      721 gctaattcca cacttcccga tgacagtcag catgacggac aatcggtgcg ttttctctat
      781 aacaaatcgc tcaatgagtc aggcacgaat attcagttag tgggttaccg ttattcgacc
      841 agtggatatt ttaatttcgc tgatacaaca tacagtcgaa tgaatggcta caacatcgaa
      901 acacaggatg gtgttattca ggttaagccg aaattcaccg actattacaa tctcgcttat
      961 aacaaacgcg ggaaattaca actcaccgtt actcagcaac tcgggcgcac atcaacactg
     1021 tatttgagtg gtagccatca aacttattgg ggaacgagta atgtcgatga gcaattccag
     1081 gctggtttaa atactgcatt cgaagatatc aactggacgc tcagctatag cctgacgaaa
     1141 aacgcctggc aaaaaggacg ggatcagatg t
//
LOCUS       DAESHI010000286         1171 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.289, whole genome shotgun sequence.
ACCESSION   DAESHI010000286 DAESHI010000000
VERSION     DAESHI010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.289"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            12..1139
                     /locus_tag="KW011_004497"
     CDS             12..1139
                     /locus_tag="KW011_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSso6 family transposase"
                     /protein_id="HBH7662641.1"
                     /translation="MNNNNTLYVGLDVHKESITVAYAINSEPVELMGKIGTSPTDIQN
                     LCKRLRSKSSQVSIVYEAGPCGYGLYRRLVKSGFDCMVCAPSLIPKKPGERVKTDRRD
                     AIRLVRSLRAGDLSAVYVPGIEDEAFRDLARAWASARDDLRHARQRLKSFLLVHGVHY
                     VGRADWGPAHRRWLSKYSFESPWRQLAFDEHRRTIEDRQAQCERLESALKEAVTEWRL
                     YPVVEALQAMRGIQFITAVGLISELGDLTRFEHPRQLMSWFGITPSEYSSGGSRHQGS
                     ITKAGNSYARKLLVEAAWSYRHPARISPAIQKRQENLPRPVIDRAWDAQLRLCKRYRK
                     LQAKGKNVNITIVAVARELAGFIWDMGRIAMSVAQQPQCHK"
BASE COUNT          311 a          272 c          309 g          279 t
ORIGIN      
        1 cggaggccag catgaacaac aataacacat tgtacgtcgg gttagatgtt cacaaagaat
       61 cgattactgt cgcttatgct atcaattcag aacctgttga actgatgggt aaaattggca
      121 catcacctac tgatattcag aatctttgta aacgtctcag gtcaaagtca tcgcaggtca
      181 gtatcgttta tgaagcgggg ccctgtggtt atggacttta tcgtcggctg gtgaaatccg
      241 gttttgactg catggtctgc gccccctcac ttatcccgaa aaaaccgggg gagcgggtta
      301 aaaccgatcg ccgtgatgcc atcagacttg tgcgttcact gcgtgcagga gatctttctg
      361 ctgtctacgt acccggcatt gaagatgagg cattccggga tttggcccgg gcatgggcat
      421 ctgcccgcga tgatttacgg catgcaaggc aacgtctgaa atcgtttctt ctggttcatg
      481 gggtccatta tgtcgggcga gcagattggg gccctgcaca ccgacgctgg ctcagcaaat
      541 actcattcga aagtccctgg cgacaattag cttttgacga acaccgtcgt acaatagaag
      601 accggcaggc acaatgtgaa cggctggaat ccgccctgaa ggaggccgtt accgaatgga
      661 ggctgtatcc ggtagtagag gcgctgcaag ccatgcgagg gattcagttt attactgcgg
      721 ttggattaat ttctgaactg ggtgatctga cccgttttga acatccacga caactgatgt
      781 catggtttgg tatcacgcca tcagaatatt catcgggtgg aagcagacat cagggcagca
      841 ttaccaaagc cggaaacagc tatgccagaa agctattggt cgaggcagcg tggagttacc
      901 gtcatccggc acgcatcagt ccggcaatac aaaaaaggca ggaaaattta ccccgccccg
      961 tcattgacag agcatgggat gctcaactca ggctttgtaa gaggtatcga aaacttcagg
     1021 ccaaaggaaa gaatgtcaat attacaattg ttgctgttgc acgtgagctg gcgggtttta
     1081 tctgggatat gggccgaata gcaatgtctg tcgcacaaca accacaatgt cacaaataaa
     1141 aaatgagaat aaccagttcc ctgaaggcga c
//
LOCUS       DAESHI010000287         1166 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.290, whole genome shotgun sequence.
ACCESSION   DAESHI010000287 DAESHI010000000
VERSION     DAESHI010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.290"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..>1166
                     /gene="istA"
                     /locus_tag="KW011_004498"
     CDS             <1..>1166
                     /gene="istA"
                     /locus_tag="KW011_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HBH7662642.1"
                     /translation="WMEYKAQAGDKAMGYSHFCRCYRKWKKTRRLSMRQEHRAGEKLF
                     IDFCGPTVPVINPDTGEIRRVAIFVAVMGASNYTYVEACEGQDMMSWLNAHSRCLTFL
                     GGVPKLLIPDNLRSAVKKADRYEPVINDSYQALAEHYGTVIIPARPRKPKDKPKAENG
                     VLIVERWLLARIRNETFHTLRALNARLRELLTDMNNRPMKGYGNQTRAERFRMLDAPA
                     LSPLPLEPYEYTEYKAVKVGPDYHVEYARHWYSVPHELVGQRLSLKVGQSVVQLWHKG
                     QCVAQHPRSTHEYKHTTNPLHMPERHRRHGTWTPERLIEQGNRTGPSTGRVVESMLKA
                     KPHPELAYRAVLGLLALQKKYGPERLEKACYVALHYNAPDRRFIDNLLRHHRDN"
BASE COUNT          292 a          334 c          314 g          226 t
ORIGIN      
        1 gtggatggaa tacaaggcac aggcgggtga caaagcgatg ggatactcac acttctgccg
       61 ttgctaccgg aagtggaaaa aaacgcgacg cctctccatg cgacaggagc atcgtgctgg
      121 cgaaaagctc tttatcgact tctgtggtcc tacggtccct gttattaatc cggacaccgg
      181 agagatacgc cgcgtggcaa tcttcgtggc tgtaatgggg gcgtccaact atacctatgt
      241 tgaagcgtgc gaaggtcagg acatgatgtc atggttaaat gcccacagcc gttgcctgac
      301 cttcctgggc ggcgtaccga agctgctcat ccccgataac ctcagaagcg cagtgaaaaa
      361 agccgatcgc tatgagcctg tcatcaacga cagttatcag gcgctggctg agcactatgg
      421 cacggttatt atcccggccc gcccgcgcaa acctaaagat aaaccaaaag cagaaaacgg
      481 cgtcctcatc gtcgagcgat ggcttcttgc ccgcatccgt aatgaaacct tccacacgct
      541 cagggcgctg aatgcacgcc ttcgggagtt gctgacggac atgaataacc gaccaatgaa
      601 gggatacggt aatcaaaccc gcgcagaacg cttccgcatg ctggatgcac cagcactctc
      661 tccgctgcct ctggagcctt atgaatacac ggaatacaaa gccgtgaagg ttggccctga
      721 ctaccacgtt gaatatgccc gtcactggta ctccgttcca cacgaactgg tcggtcagcg
      781 tctgtcactg aaggtggggc agtcggtggt acagctctgg cacaaggggc agtgtgtggc
      841 gcaacatccg cgcagcacac atgaatataa acacacaacc aatccgcttc acatgccaga
      901 acgccaccgg cggcatggga catggacacc ggaacgcctt atcgagcagg gaaaccgtac
      961 aggtccgtcc acaggccggg tggttgaatc catgctgaaa gccaaacccc atccggagct
     1021 ggcttaccgt gcagtcctgg gtctgctggc acttcagaaa aaatacggcc cggaacggct
     1081 ggaaaaggcc tgttacgttg ccctgcacta taacgccccg gacagacgct ttatcgacaa
     1141 tctgctgcgt catcatcggg ataacg
//
LOCUS       DAESHI010000288         1161 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.291, whole genome shotgun sequence.
ACCESSION   DAESHI010000288 DAESHI010000000
VERSION     DAESHI010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.291"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            248..493
                     /locus_tag="KW011_004499"
     CDS             248..493
                     /locus_tag="KW011_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662643.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPIVGIRQIRLIT"
     gene            complement(510..1142)
                     /locus_tag="KW011_004500"
     CDS             complement(510..1142)
                     /locus_tag="KW011_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HBH7662644.1"
                     /translation="MDPLHAVYLTVGLFVITFFNPGANLFVVVQTSLASGRRAGVLTG
                     LGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAWCSMRRQSTPQMST
                     LQQPISASWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTWARLMAWAGIVLAS
                     VIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYEGVTQR"
BASE COUNT          351 a          277 c          275 g          258 t
ORIGIN      
        1 gtttcgcagc catgcggtgt ataaaaaatg atctcatgca gatgttttgt gaatgtgtgg
       61 gttgacattc atatgaaaaa aatcataatt ccatcatgtt tgtgtggggt cttttctgta
      121 ccttacgcat cgcactcaag cctgacagaa aatatgctgt aaggctcata tcaaaaaccg
      181 ccattagctc atcaggaaga gcagacgaca accaggattg ttgtatggta cggggttcga
      241 ggcctcgatg gcggtccaac ttttcaaaac tcttctgaat cagatcccct tattatcatc
      301 cctgcaaagt ggaacattgc cattgtttgg ctattcagga cgggtcagac caatgaaaaa
      361 agcccacaca ggggagagtg ggctgaaatg ggaagctaaa gactcaagta aacttatcgg
      421 aaataaggac cacgcattac gggggctatc atcgccgata gtcgggattc ggcaaatcag
      481 gttgattacg tagagagtaa attatctgct caccgctgcg tcaccccttc gtaaatcagg
      541 cgtagcgcga atacaccaat aattgcacca ataacccgac tggcaacgcg ttgcatacgc
      601 ccataagcac gacgcacagc gggcaaagaa aacgcctgac taagaaagac tcgccagata
      661 actgatgcga gcacaatccc cgcccaggcc attaaacgtg cccatgttgg tgtttcggca
      721 tttaatgtta ctgagaaaat actgataaaa aataaaacgg tttgcgggtt agagagatcg
      781 gtaattaatc cgcggcgaaa aaagacatac caggaggcgc taatcggttg ttgtagtgtg
      841 ctcatttgcg gtgttgactg gcggcgcatg ctgcaccacg caaaccataa gagataagcg
      901 ccgccgacga ttctgataag cgaaaaaatc tcctcacact gcgttattag cgttgcaaga
      961 ccaaacaaac ccaacccgga ataaaatgca tcgcccagcg ccacgcccag tccggtcagc
     1021 acccctgcgc gtcgaccgga agccaggctg gtttgtacta ccacaaagag attggctccc
     1081 ggattaaaaa aagtaatcac gaacagtcct acggtcaggt aaacggcgtg caaaggatcc
     1141 atagtgattt catccataaa t
//
LOCUS       DAESHI010000289         1157 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.292, whole genome shotgun sequence.
ACCESSION   DAESHI010000289 DAESHI010000000
VERSION     DAESHI010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.292"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            6..407
                     /locus_tag="KW011_004501"
                     /pseudo
     CDS             6..407
                     /locus_tag="KW011_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            572..>1157
                     /locus_tag="KW011_004502"
     CDS             572..>1157
                     /locus_tag="KW011_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBH7662645.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRV"
BASE COUNT          322 a          260 c          276 g          299 t
ORIGIN      
        1 ggtgacgagt ctcatcgtct atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tgtgggcccg tcgcccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt
      121 cactggccta taaggcgcga ctaaaagaag caaaactgct ggcatcaaca gggagtactg
      181 gtgactcgta tgacaacgcg atggcagaga tcatcaaggg cctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccggacag aagtggagct ggccacactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tatcccaccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaatg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcag ttcacaaaaa cacattaatg cagtaactat
      481 attttccttt ctggtgataa cgtcgggtta tcattagctt cttcagctat tttactttta
      541 tatcccttgt aaacatcatg gagaatccat agtgaataaa gtttattctc ttaaatattg
      601 ccccgtcacc ggggggctta ttgctgtctc tgaacttgcc cgcagggtaa taaaaaagac
      661 atgccgaaga ttaacgcata ttcttctggc tggcattcca gcaatctgtc tgtgttactc
      721 tcagatatcc caggcgggta ttgtccgttc cgatattgcc tatcagattt atcgtgattt
      781 cgccgaaaac aaagggcttt ttgtacctgg tgccaatgat attccggtat atgataagga
      841 cggaaaactt gtgggaagac tgggtaaagc cccaatggcc gatttcagca gtgtgagctc
      901 aaatggcgtt gctacgcttg tatcgcctca gtatatcgtc agcgtaaagc ataacggagg
      961 atatcggagt gtgagctttg gtaatgggaa aaatacatat tcccttgttg accgtaataa
     1021 ccacccttct attgacttcc atgctccacg tctgaataaa ctggttacag aagttattcc
     1081 ctcagcggta acatcagaag gaaccaaagc caatgcttat aaatacactg aacgttacac
     1141 cgctttttat cgggtgg
//
LOCUS       DAESHI010000290         1145 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.293, whole genome shotgun sequence.
ACCESSION   DAESHI010000290 DAESHI010000000
VERSION     DAESHI010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.293"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..187
                     /locus_tag="KW011_004503"
     CDS             <1..187
                     /locus_tag="KW011_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBH7662646.1"
                     /translation="REYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNN
                     YRIALRKYQHYQRQTCT"
     gene            320..1120
                     /gene="tcyJ"
                     /locus_tag="KW011_004504"
     CDS             320..1120
                     /gene="tcyJ"
                     /locus_tag="KW011_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH7662647.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
BASE COUNT          318 a          248 c          306 g          273 t
ORIGIN      
        1 gcgtgaatat gtcgttcgtc tgcgccgttt gggtaatcat ctacacgagc aaaatatttc
       61 cctcgatcag ctgcaggacg gtttccttga tgaaatcctc gccccgtggc tgcccaccac
      121 cagcaccaac aattaccgca ttgcgttacg gaagtatcaa cactatcagc gtcaaacgtg
      181 tacctgactt gtgcagaaat cctccagtct gcctgcttct gatatatatt aaataagaat
      241 aagatgtagc ggagttgttt ttgtgtttac aaacaatggc tctacactgc aaacagacat
      301 aacaacattc ggggtgaata tgaaattagc acatctggga cgtcaggcat tgatgggtgt
      361 gatggccgtg gcgctggttg cgggtatgag cgttaaaagt tttgcagatg aaggtctgct
      421 taataaagtc aaagagcgcg gcacgctgct ggtagggctg gaaggaactt atccgccgtt
      481 cagttttcag ggggatgacg gcaaattaac cggttttgaa gtggaatttg cccaacagct
      541 ggcaaaacat cttggcgttg aggcgtcact aaaaccgacc aaatgggacg gtatgctggc
      601 gtcgctggac tctaaacgca ttgatgtggt gattaaccag gtcaccattt ctgacgagcg
      661 caagaaaaaa tacgattttt ctacccctta caccatttcc ggtattcagg cgctggtgaa
      721 aaaaggtaac gaaggcacca ttaaaacagc cgatgatctg aaaggcaaaa aagtgggtgt
      781 cggtctgggc accaactatg aagagtggct gcggcagaat gttcagggtg tcgatgtgcg
      841 tacctatgat gatgacccga ccaaatatca ggatctgcgc gtaggccgta tcgatgcgat
      901 cctcgttgac cgtctggcgg cgctggatct ggtgaagaaa accaacgata cgctggcagt
      961 aaccggtgaa gcattttccc gtcaggagtc tggcgtggcg ctgcgtaaag gaaatgaaga
     1021 tctgctgaaa gcagtgaatg atgcaattgc ggaaatgcaa aaagatggca ctctgcaagc
     1081 cctttccgaa aaatggtttg gtgctgatgt gaccaaataa tcagcataat gacaaaaaag
     1141 ggcgc
//
LOCUS       DAESHI010000291         1141 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.294, whole genome shotgun sequence.
ACCESSION   DAESHI010000291 DAESHI010000000
VERSION     DAESHI010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.294"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            35..190
                     /locus_tag="KW011_004505"
                     /pseudo
     CDS             35..190
                     /locus_tag="KW011_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            294..1109
                     /locus_tag="KW011_004506"
                     /pseudo
     CDS             294..1109
                     /locus_tag="KW011_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          325 a          234 c          272 g          310 t
ORIGIN      
        1 ataacagatg tctggaaata taggggcaaa tccaggtact gccgaaaata ttcagataga
       61 gctgagggat gaccaggatg ctgcgttaaa aaatggcgat agcaaaacgg ttattgttga
      121 tgagatcact cgtaatgcac agtttccact taaggcaaga gctatcacgg tgaatggaaa
      181 cgcaagctag ggaacgatcg aggcgctaat caatgtgatc tacacctggc aataacgtcg
      241 attcgatttt cttagccttc tttttaaatc aatttttaac cttaggatat atcatgggta
      301 aaacaatcag tataaaggtc ctgttcggta tttatcttct attaatggca ggcaaagttt
      361 ttgccttcag ttgtaatgtc gatggcgggt caagtattgg agcaggtaca acctctgttt
      421 atgttaatct cgaccctgta atacagccgg gccagaatct ggttgtagac ttgtctcagc
      481 atataagttg ctggaatgat tacggcggct ggtacgacac tgatcatata aacctggtac
      541 aaggctcagc tttcgcaggc tccctacagt catataaagg atcgctgtac tggaataatg
      601 tgacctaccc cttcccgttg acgacaaata ccaacgtatt ggatattggt gataagacac
      661 cgatgccatt accgttaaaa ctctatatta ccccagttgg ggcggccggt ggagttgtca
      721 taaaagcggg ggaagtcatt gcccgtattc atatgtataa aattgcaaca ttaggaagcg
      781 gaaatccgcg taattttacg tggaatatta tttcaaataa cagtgttgtt atgccaacag
      841 gcggctgcac cgttgatagt cgtaatgtca ctgtggatct cccggacttt cctggaagcg
      901 cagaaatacc ccttggcgta tattgctcaa gcgaacaaaa gctctcattt tatctttcgg
      961 gtgccaccac tgacagtagc cgacaagtgt ttgctaacac agcacctgat gcaacaaaag
     1021 catcgggagt gggtgtcact ctaatgcgta atggaaaaat actggctacg ggagaaaatg
     1081 tctccctggg gactaatgcc agtcagttaa gcaactgact ggctcttttt cggggctgtg
     1141 g
//
LOCUS       DAESHI010000292         1140 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.295, whole genome shotgun sequence.
ACCESSION   DAESHI010000292 DAESHI010000000
VERSION     DAESHI010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.295"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..>1140
                     /locus_tag="KW011_004507"
     CDS             <1..>1140
                     /locus_tag="KW011_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBH7662648.1"
                     /translation="EREIDHLQAQLDKLRRMNFGSRSEKVSRRIAQMEADLNRLQKES
                     DTLTGRVYDPAVQRPLRQTRTRKPFPESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTA
                     EQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERGIAGPGLLARVLTSKYAE
                     HTPLYRQSEIYGRQGVELSRSLLSGWVDACCRLLSPMEEALHGYVLTDGKLHVDDTPV
                     PVLLPGNKKTKTGRLWAYVRDDRNAGSTLAPAVWFAYSPDRKGIHPQTHLAGFSGVLQ
                     ADAYAGFNELYRDGRITEAACWAHARRKIHDVHVRTPSALTEEALKRIGELYAIEAEI
                     RGMTAEQRLAERQLKTKPLLKSLESWLREKMKTLSRHSELAKAFA"
BASE COUNT          250 a          323 c          361 g          206 t
ORIGIN      
        1 ggagcgcgaa atagaccatc tgcaggcgca gctggataaa ctccgccgga tgaacttcgg
       61 cagtcgttcc gaaaaagtct cccgccgtat cgcacaaatg gaagccgatc tgaaccggct
      121 tcagaaagag agcgatacgc tgactggtag ggtgtatgac ccggcagtac agcgtccgtt
      181 gcgtcagacc cgcacccgta agccgttccc tgaatcacta ccccgtgacg aaaagcgact
      241 gttgcctgcg gcgccgtgct gcccgaactg cggcggttca ctgagctatc tgggcgagga
      301 taccgccgaa cagctggagt tgatgcgtag cgccttccgg gttatccgga cggtacggga
      361 aaaacatgcc tgtactcagt gcgatgccat cgtgcaggca cctgcacctt cgcggcccat
      421 cgagcggggt atcgccggac cggggctgct ggcccgcgtg ctgacctcga agtatgcaga
      481 gcacaccccg ctgtatcgcc agtcagaaat atacggccgg caaggtgtgg agctgagccg
      541 ttcactgctg tcgggctggg tggatgcatg ctgccggctg ctgtctccga tggaagaggc
      601 gcttcatggc tatgtgctga ctgacggtaa gctccatgtt gatgacacgc ctgtcccggt
      661 gctgttgcca ggcaataaga aaacgaagac cgggcggttg tgggcgtatg ttcgtgacga
      721 ccgtaacgcc gggtcaacgc tggcgccggc ggtgtggttc gcttacagcc cggacagaaa
      781 aggcatccat ccgcagaccc atcttgcggg gttcagtggt gtactgcagg cggatgcata
      841 cgccgggttc aacgagctgt accgggatgg ccggataacg gaagccgcct gttgggctca
      901 cgcccgccgt aaaatccacg atgtgcacgt tcgcaccccg tcagccctga cggaggaagc
      961 gctgaaacgg atcggcgaac tgtacgccat cgaggcagag ataaggggaa tgacggcgga
     1021 acagcgcctt gccgaacgtc agttgaaaac gaaaccgctg ctgaaatccc tggaaagctg
     1081 gctgcgtgaa aagatgaaaa ccctgtcgcg acactcagaa ctggcgaaag cgttcgcata
//
LOCUS       DAESHI010000293         1117 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.296, whole genome shotgun sequence.
ACCESSION   DAESHI010000293 DAESHI010000000
VERSION     DAESHI010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.296"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            84..1115
                     /locus_tag="KW011_004508"
     CDS             84..1115
                     /locus_tag="KW011_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HBH7662649.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT          303 a          285 c          301 g          228 t
ORIGIN      
        1 tagatcactt tgagggaact tagcccggat tgtgcgatct gatcaatcgc caaatcaaaa
       61 caaatcacca accggactga gcaatgccga tcatagcacc aatttcccgt gacgaacgac
      121 gcctgatgca gaaagccatc cataaaacac acgataaaaa ttatgcccgc agactgactg
      181 ccatgctgat gctgcaccgg ggcgaccgtg tcagcgacgt tgccagaacg ctctgctgcg
      241 cccgttcctc tgttggacgc tggattaact ggttcacgca gtcgggtgtt gagggactga
      301 aatcattacc tgccgggcgt gcccgtcgct ggccgtttga gcatatctgc acactgttac
      361 gtgagctggt aaaacattct cccggcgact ttggctacca gcgttcacgc tggagtacag
      421 aactgctggc aataaaaatc aatgagataa ccggttgcca gttaaatgcc ggaaccgttc
      481 gccgctggtt gccgtctgcg gggattgtgt ggcgaagggc tgcgccaact ctgcgtatcc
      541 gtgacccgca taaagatgaa aagatggcag caatccataa agcactggac gaatgcagcg
      601 cagagcatcc ggtcttttat gaagatgaag tggatatcca tcttaatccc aaaatcggtg
      661 cggactggca actgcgcgga cagcaaaaac gggtggtcac gccgggacag aatgaaaaat
      721 attatctggc cggagcgctg cacagcggga caggtaaagt cagctgtgtg ggcggcaaca
      781 gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct taaagcgaca taccgtcggg
      841 cgaaaaccat cacgctgatc gtggacaact acattatcca caaaagccgg gaaacacaga
      901 gctggctgaa ggagaacccg aagttcaggg tcatttatca gccggtttac tcgccatggg
      961 tgaatcatgt tgaacggcta tggcaggcac ttcacgacac aataacgcgt aatcatcagt
     1021 gcagctcaat gtggcaactg ttgaaaaaag ttcgccattt tatggaaacc gtcagcccat
     1081 tccccggagg caaacatggg ctggcaaaag tgtagcg
//
LOCUS       DAESHI010000294         1116 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.297, whole genome shotgun sequence.
ACCESSION   DAESHI010000294 DAESHI010000000
VERSION     DAESHI010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.297"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            6..1116
                     /locus_tag="KW011_004509"
                     /pseudo
     CDS             6..1116
                     /locus_tag="KW011_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          275 a          323 c          347 g          171 t
ORIGIN      
        1 cgtagggtga agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca
       61 cgcagagtca gtttgacgcc gtgggcaggc tcaggacaca gacggatgcc gcaggccgga
      121 caacagaata cagcccggat gtggtgacgg gcctcatcac gcgcatcacc acgccggatg
      181 gcagggcatc ggcgttttac tataaccacc acagccagtt aacgtcagcc accgggcctg
      241 acgggctgga aatgcgccgc aaatatgatg aatccggccg tctgattcag gaaaccgccc
      301 ctgacggcga tatcacccgc taccgttatg ataatccaca cagtgactta ccctgcgcaa
      361 cggaagatgc caccggcagc cggaaaacca tgacatggag ccgttacggt cagttgctga
      421 ccgtcactga ctgttccggt tatgtaaccc gttatgacca tgaccgtttt ggtcaggtga
      481 cggcggtgca ccgcgaggaa gggctgagcc agtaccgcgc atacgacagc cgtggacagt
      541 taattgcggt gaaagacacg cagggccatg aaatgcggta tgaatacaac gccgccggtg
      601 acctgaccgc cgtcattgcc ccggacggca gcagaaacgg gacacagtac gatgcgtggg
      661 gaaaagccat ctgtaccacg cagggcgggc tgacgcgcag tatggaatac gatgctgccg
      721 gacgggtcat ccgtctgacc agtgaaaacg gcagccacac caccttccgt tacgatgtac
      781 tcgaccggct gatacaggaa accggctttg acggccgcac acagcgttat caccacgacc
      841 tgaccggcaa acttatccgc agcgaggatg aggggctggt cacccactgg cactatgacg
      901 aagcagaccg cctcacgcac cgcaccgtga agggtgaaac cgcagagcgg tggcggtacg
      961 acgaacgcgg ctggctgaca gacatcagcc atctcagcga agggcaccgg gtgacggtgc
     1021 attacgggta tgatgagaaa ggccggctga ccggtgagcg tcagacggtg catcacccgc
     1081 agacggaagc actgctctgg cagcatgaga ccagac
//
LOCUS       DAESHI010000295         1115 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.298, whole genome shotgun sequence.
ACCESSION   DAESHI010000295 DAESHI010000000
VERSION     DAESHI010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.298"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..643)
                     /locus_tag="KW011_004510"
     CDS             complement(<1..643)
                     /locus_tag="KW011_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBH7662650.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSAFTTMGTRQGHTAIIFQQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTIT"
     gene            complement(766..>1115)
                     /gene="tabA"
                     /locus_tag="KW011_004511"
     CDS             complement(766..>1115)
                     /gene="tabA"
                     /locus_tag="KW011_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HBH7662651.1"
                     /translation="LSISWSHYLFYLISEDMTEPHEARRTEYHARYLDIQIVLKGQEG
                     MTFSTQPAGTPDTDWLADKDIAFLPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAP
                     AQVRKAVVKMLMA"
BASE COUNT          322 a          252 c          197 g          344 t
ORIGIN      
        1 ccgtaatcgt agaatcaact gaatctttac aactcacctg cgagttattc aagtcctgag
       61 aattatttaa ctcctgcgga atatttaata gacgagctat ttccttgtta gcttcgcgtt
      121 ggtaacatgc cagaatcttg ttagtaatca ggtgtaatga actctcatct tttctttcaa
      181 aggactttcc ttcataacga ttagtcgaac catcaggttg ttgaaatata atagccgtat
      241 gtccttgacg agtccccata gtagtgaaag cggaaagtgt gatttctatt tccaccttag
      301 ataaatccgc tcgttcccct tcaggattat tcataacaaa cttcattatc tcactgccat
      361 cattattttt gatataagat gaggactcaa tatgagccat catatttctt cccgcttcgt
      421 tggagcgttt gctaaaatcc acataaccta gagtaaatat ttttaccaaa aattccaaaa
      481 atgtcttagg atcaaggata tttcttgtgt tatcctgccc ctcggcatta cgggcaaaat
      541 tattcattcc aacacgccag gtatttaaaa cttcactttt ttgtttaaaa ttgtcggcta
      601 atgtaatgtg ttgagaataa ttcaaatctg atattttgct catgacgaac tccttgagtt
      661 aataagaacg tctaagcatt acaaatttta ctcacaaaaa aatatcaaaa aacgctcttt
      721 tcacaaactc gccgtaaacc tggagttatt tacggcgaaa agtcatcacg ccatcagcat
      781 cttcactact gctttgcgaa cctgggctgg tgcacccact gcgcacagcg gtttatgcac
      841 ttcccccgga taaaacacaa caaaatcacc ttcattaagg ataactgttt tctcatcaac
      901 gccttccggc aaaaatgcga tgtctttatc agctaaccag tcggtatccg gcgtgcctgc
      961 aggttgcgtg ctgaaggtca tgccttcctg accttttaac acaatctgaa tgtcgagata
     1021 gcgggcatgg tactccgtac ggcgagcttc gtgcggctcg gtcatatctt ccgagataag
     1081 ataaaacagg taatgactcc aacttattga tagtg
//
LOCUS       DAESHI010000296         1112 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.299, whole genome shotgun sequence.
ACCESSION   DAESHI010000296 DAESHI010000000
VERSION     DAESHI010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.299"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            212..1087
                     /gene="nadA"
                     /locus_tag="KW011_004512"
                     /pseudo
     CDS             212..1087
                     /gene="nadA"
                     /locus_tag="KW011_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
BASE COUNT          301 a          263 c          273 g          275 t
ORIGIN      
        1 caggcatcct caatttcact ttgtaaacct gatgacatcg tcagagctta ctgtgcaagc
       61 aactctacgt cggtggaatt aggcgtaatt cgagtgactt ttccccacca ttcgactatc
      121 ttgtttagca tataaaacaa attacgccga tagtagcgaa tattacgcta atgtcggttt
      181 taacgttaag cctgtaaaac gagatggtaa gatgagcgtg atgtttgatc cagacacggc
      241 gatttatcct ttccccccga agccgacgcc gttaagcatt gatgaaaaag cgtattaccg
      301 cgagaagata aaacgtctgc taaaagaacg taatgcggtg atggttgccc actactatac
      361 cgaccccgaa attcaacaac tggcagaaga aaccggtggc tgtatttctg attctctgga
      421 aatggcgcgc ttcggtgcaa agcatcccgc ttctacgttg ttagtcgctg gggtgagatt
      481 tatgggagaa accgccaaaa ttctcagtcc ggaaaaaaca attctgatgc tgacacttca
      541 ggctgaatgt tcactggatc tcggctgccc tgttgaagaa tttaacgcat tttgcgatgc
      601 ccatcccgat catactgtcg tcgtctacgc caacacttct gctgcggtaa aagcgcgcgc
      661 agattgggtg gtaacttcaa gcattgccgt cgaacttatt gatcatctgg atagttttgg
      721 tgaaaaaatc atctgggcac ccgacaaaca tctggggcgt tacgtgcaaa aacagacggg
      781 cgcagacatt ttatgctggc agggtgcctg tattgtgcat gatgaattta agactcaggc
      841 gttaacccgc ttgcaagaag aatacccgga tgctgccata ctggtgcatc cagaatcacc
      901 acaagctatt gtcgatatgg cggatgcggt cggttccacc agtcaactga tcgctgctgc
      961 gaaaacattg ccacatcagc ggcttatcgt ggcaaccgat cggggcattt tctacaaaat
     1021 gcagcaggcg gtgccagata aagagttact ggaagcacca accgcaggtg agggtgcaac
     1081 ctgccgcagg tgtcagcgcc agtgatataa ga
//
LOCUS       DAESHI010000297         1111 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.300, whole genome shotgun sequence.
ACCESSION   DAESHI010000297 DAESHI010000000
VERSION     DAESHI010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.300"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..>1111
                     /locus_tag="KW011_004513"
     rRNA            <1..>1111
                     /locus_tag="KW011_004513"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          295 a          234 c          363 g          219 t
ORIGIN      
        1 tcctgactga ccgatagtga accagtaccg tgagggaaag gcgaaaagaa ccccggcgag
       61 gggagtgaaa aagaacctga aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg
      121 tgactgcgta ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga
      181 ataggggagc cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc
      241 gaaacccggt gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg
      301 aaccgactaa tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa
      361 accgggagat agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc
      421 cgggggtaga gcactgtttc ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact
      481 gcgaataccg gagaatgtta tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa
      541 gagggaaaca acccagaccg ccagctaagg tcccaaagtc atggttaagt gggaaacgat
      601 gtgggaaggc ccagacagcc aggatgttgg cttagaagca gccatcattt aaagaaagcg
      661 taatagctca ctggtcgagt cggcctgcgc ggaagatgta acggggctaa accatgcacc
      721 gaagctgcgg cagcgacgct tatgcgttgt tgggtagggg agcgttctgt aagcctgcga
      781 aggtgtgctg tgaggcatgc tggaggtatc agaagtgcga atgctgacat aagtaacgat
      841 aaagcgggtg aaaagcccgc tcgccggaag accaagggtt cctgtccaac gttaatcggg
      901 gcagggtgag tcgaccccta aggcgaggcc gaaaggcgta gtcgatggga aacaggttaa
      961 tattcctgta cttggtgtta ctgtgaaggg gggacggaga aggctatgtt ggccgggcga
     1021 cggttgtccc ggtttaagcg tgtaggctgg ttttccaggc aaatccggaa aatcaaggct
     1081 gaggcgtgat gacgaggcac tacggtgctg a
//
LOCUS       DAESHI010000298         1087 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.301, whole genome shotgun sequence.
ACCESSION   DAESHI010000298 DAESHI010000000
VERSION     DAESHI010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.301"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            528..>1087
                     /gene="fimB"
                     /locus_tag="KW011_004514"
     CDS             528..>1087
                     /gene="fimB"
                     /locus_tag="KW011_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HBH7662652.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTTSNAGRFYG"
BASE COUNT          333 a          189 c          233 g          332 t
ORIGIN      
        1 ccaggataaa aaggaatgta acaatctcat ggcgtaagct gacgaaccag gcaggaataa
       61 tcgctaggga cctaagcatt agcatgataa tagccactaa gaaattactg tgttccatga
      121 aatagccatt ttgtggcaaa tggagttgac taataatgtc atatgtgaga cggctagttg
      181 aacgaatatt aaattttgct gaatttttta tgttgatttt acttgttaca gaacatatca
      241 catgatatat agataagatt agttgcatta atgatgaggg ttattattag attcgtatca
      301 ggttgataaa tatataaagg tacatagcat gcaagagcat ggcgtttgta tggcaaagtt
      361 attataagta actatcgcta ctccatttaa gttcgctcag aagaactggt ccacttacgt
      421 tagttattaa gcaaacgttc gcttttataa acataatcag gattaaaatg ttggattatt
      481 gctaacccag cacagctagt gcgcgtctgt aattataagg gaaaacgatg aagaataagg
      541 ctgataacaa aaaaaggaac ttccttaccc atagtgaaat cgagtcactc cttaaagcag
      601 caaataccgg gcctcatgca gcacgtaatt attgtctgac tttgctttgt tttattcatg
      661 gtttccgggc gagtgaaatt tgtcgattga ggatttcgga tattgatctt aaggcaaagt
      721 gtatatatat ccatcgatta aaaaaaggct tttcaacaac gcacccgcta ttgaacaaag
      781 aagttcaggc tttaaaaaac tggttgagta tccgtacttc ttatccgcat gctgagagcg
      841 agtgggtatt tttatcacgt aaggggaatc cgctttctcg gcaacagttt taccatatta
      901 tctcgacttc cggtggtaat gccgggttgt cactggagat tcatccgcac atgttacgcc
      961 attcgtgtgg ttttgctttg gcgaatatgg gaatagatac gcgacttatc caggattatc
     1021 ttgggcatcg caatattcgt catactgtct ggtataccac cagcaatgca gggcgttttt
     1081 acggcat
//
LOCUS       DAESHI010000299         1086 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.302, whole genome shotgun sequence.
ACCESSION   DAESHI010000299 DAESHI010000000
VERSION     DAESHI010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.302"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(506..>1086)
                     /locus_tag="KW011_004515"
     CDS             complement(506..>1086)
                     /locus_tag="KW011_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH7662653.1"
                     /translation="RLYLAEMLYYPIDLIEKFQNLYAITDQIRNTTGFCLPQNSELIY
                     CWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSKQSLISRCYNLMLSEP
                     GTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNALSAIQTTVKPISEIA
                     RENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT          343 a          207 c          190 g          346 t
ORIGIN      
        1 atctacttca caatttaaat gagaaactaa aacttacatc ttgaaataat ctcattgatt
       61 ggatgaatat ttgtagtgca gtgacatcat ttttaaataa tagttcaaat aaaagggctc
      121 acgatgaaaa aattaacagt ggcaatttct gctgtagctg catcttaacg tataaactag
      181 atttaataat aacgaactag atttaataac aacgatatcc gtacctatcc ggatatctaa
      241 tgtcataagc atccctttaa ttatcccctc aacctcttgt aactttccca ctcttgaact
      301 ccacatagtc caaacgccgg atgcaaaaaa atcacacgtg catttctgaa attgtgatgg
      361 aggaagatag tatcaattat atgtgcttat aaacgtcccc gattagcgca gaagtcaccg
      421 gattgttcag gctccaatag gttgagtatg ccgtatttat tttccaaaat aaaaatcgat
      481 gctgttgctc cttcataaaa tacttttact ctctggacgc ttttcttagc tctcttggtg
      541 ttatcttaaa acgattatgg aacctttcag taaaacgaga aggacacttg taaccatttt
      601 ccctggcaat ctcgctgatg ggttttactg tcgtttgtat agcagacaac gcattattta
      661 acctcacgtc gtccaatata ctttgaaaac ttactccctc gcttgccaga cggcgatgca
      721 atgtggaaac agaaatgtag agatatctcg ctaccttgtt tgctgtccat tttgttccag
      781 gttcggatag cattaggtta taacaacgac ttattaatga ttgtttacta tctgataaaa
      841 gtaaacaatt aacatgatgt gctcctaatg aaagtagaac gcccattgct aagtgttcct
      901 gaatttgcgt tgagaagcct cgggaaatag atgtttttag ttgctcccaa caatatatta
      961 actcggaatt ctgaggtaag cagaaacctg tcgtgttacg aatttgatcg gttatcgcat
     1021 aaagattttg gaacttttca attagatcaa tgggataata aagcatttct gcaagataaa
     1081 gacggt
//
LOCUS       DAESHI010000300         1083 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.303, whole genome shotgun sequence.
ACCESSION   DAESHI010000300 DAESHI010000000
VERSION     DAESHI010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.303"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..>1083
                     /gene="tssI"
                     /locus_tag="KW011_004516"
     CDS             <1..>1083
                     /gene="tssI"
                     /locus_tag="KW011_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBH7662654.1"
                     /translation="AQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTFCISQFRY
                     SAQIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFAR
                     WQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVP
                     GRSGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRV
                     KFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGR
                     TYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHVQKNMNTEVL
                     NNRTTDVINNHAEKIGNNQAITVTNNQ"
BASE COUNT          276 a          296 c          326 g          185 t
ORIGIN      
        1 gcgcaaaaaa gtaccgacca gagcctggtc ctgtgcgaca ccgtgcgtta tctgccggag
       61 tcctttgaga tcccctggaa cccgaacacc cgtaccgagg taagtacctt ctgcatcagc
      121 cagttccgct acagcgcaca aatccgtcct tcttccgtgg tgaccaaaga ctacaccttt
      181 aaacgacccg gctgggcagg gcgttttgat caggaaggcc agtaccagga ttaccagcgc
      241 acacagtatg aagtgtatga ctaccccgga cgtttcaagg gtgctcacgg gcagaacttt
      301 gcccgctggc agatggatgg ctggcgcaac aacgcagaag tggcgcgcgg aacaagccgt
      361 tctccggaga tatggccggg acggcgaatt gtgctgacgg ggcatccgca ggcgaacctg
      421 aaccgggaat ggcaggtggt ggcaagtgaa ctgcacggcg agcagccgca ggcggtgccg
      481 ggacgcagtg gttcaggaac cacgctgaat aaccactttg cggtaatacc ggcagaccgg
      541 acatggcggc cacagccgtt gctgaaaccg ctggtggatg gcccgcagag cgccgtcgtg
      601 acgggaccgg caggcgagga aatcttctgt gatgaacatg gccgcgtgcg ggtgaaattt
      661 aactgggacc gctataaccc gtcaaaccag gacagttcat gctggatccg tgtggcacag
      721 gcgtgggcag gcaccggatt cggtaacctt gcgataccgc gcgtgggtca ggaggtcatt
      781 gtggacttcc tcaacggcga tccggaccag ccgattatta tggggcgtac ctaccaccag
      841 gaaaaccgca cacccggtag cctgccgggg acgaagacgc agatgaccat tcgttcgaaa
      901 acctataagg gcagcgggtt taatgaactg aagtttgacg atgcgacagg gaaagaacag
      961 gtctacatcc acgtgcagaa gaacatgaac accgaggtgc tgaataaccg caccacggat
     1021 gtgataaaca accatgccga aaaaataggt aacaaccagg cgatcaccgt taccaataac
     1081 cag
//
LOCUS       DAESHI010000301         1054 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.304, whole genome shotgun sequence.
ACCESSION   DAESHI010000301 DAESHI010000000
VERSION     DAESHI010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.304"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..216)
                     /locus_tag="KW011_004517"
     CDS             complement(<1..216)
                     /locus_tag="KW011_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH7662655.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     VGNKYIPYGKMASWLVEWKNATETQWLK"
     gene            224..655
                     /gene="tnpA"
                     /locus_tag="KW011_004518"
                     /pseudo
     CDS             224..655
                     /gene="tnpA"
                     /locus_tag="KW011_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          317 a          240 c          200 g          297 t
ORIGIN      
        1 tttaagccat tgcgtttcag tggcattttt ccactcaacc agccaggaag ccattttccc
       61 gtaagggatg tatttattac cgacctcatg attctcattc tgacgtgcca gagcacgatt
      121 gaaaacgaaa cgacatgcgc ctgcgaagcg cctcatttca cgctcctgtt gaccaccggg
      181 tcttaactgg aatttaaatg cttgtagtcg tttcacaggc caaatgatac tttggtctgt
      241 gaaaaaagaa accgatatcc ggcgtggaag gcattgtgtt ttcctgatgc atgttcacct
      301 ggtctttgtc accaaatacc gacgtcagat ttttgactat gacgcgacag aaaaactacg
      361 cacttacttt tcaaatgtat gtgctgattt tgaagctgaa ctggttgaaa tggatggcga
      421 accagatcac gtccatttgt taattaacta tcctcccaaa ctggcgatat ccagtctggt
      481 aaacagcctc aaaggcgtat cgggtaggtg actgcgacga gatcgaccag atattgcagt
      541 caggtattac tacaaaggcg ttttgtggag tgttggctat tttgccagta gctgcggagg
      601 tgcgccaata tccgtcatcc gccaatacat tgaacaacag caaacacctg gttaggtgga
      661 aaaccacgcc ttatatccct gccctgaagg acggggtttt acggcgcacc ggataagcct
      721 ggagattatc tacaccagca tggacatcat tgtactatgc cccgtacatg ccccatcaca
      781 tcctcaacat caaagtatta gagccttgca aaccattatg cgtctcagtt tgtctcatct
      841 atcttacgca aaaaaaatag aactaatatc aagcggcgct atattgcgta ttaaataaaa
      901 ccacctcaat tacattaata aatctaaaca aacatcatgc aaataatttc catggttatt
      961 tctaggctat ttaaattaaa taaataaatt atgagtcaat cataaataac aacctttaat
     1021 ataatttaac taccaaattt atattcgttc acaa
//
LOCUS       DAESHI010000302         1035 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.305, whole genome shotgun sequence.
ACCESSION   DAESHI010000302 DAESHI010000000
VERSION     DAESHI010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.305"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            74..289
                     /gene="xisR"
                     /locus_tag="KW011_004519"
     CDS             74..289
                     /gene="xisR"
                     /locus_tag="KW011_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HBH7662656.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            291..>1035
                     /locus_tag="KW011_004520"
     CDS             291..>1035
                     /locus_tag="KW011_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HBH7662657.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEISSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKG"
BASE COUNT          348 a          191 c          243 g          253 t
ORIGIN      
        1 catagctgat tattaataat caacatcggg cgtcaatttc agcctaacat tggcgcctgc
       61 cagaggtgat gcgatggcac aagtaatctt taatgaagag tggatggttg aatacggcct
      121 gatgcttcgc actggtctgg gtgccagaca aattgaagca taccgccaga actgttgggt
      181 ggaaggcttc cacttcaaac gagtatctcc tttagggaag ccagacagta agcgagggat
      241 tatctggtac aactatccga agataaatca gtttatcaaa gactcatgat atgtctaaat
      301 taccaacagg tgtcgagatt cgaggtaaat acattcgcat ctggttcatg tttcgaggaa
      361 aacgatgtcg ggaaacattg aaaggctggg agattactaa cagtaacatt aaaaaggccg
      421 ggaatttaag agcgttgata gttcatgaaa tcagttccgg tgaatttgag tatttaagac
      481 gttttcccca gtccagcact ggggcaaaaa tggtgacaac gagggtcata aaaacattcg
      541 gggagctttg tgatatctgg acaaaaatta aagagacaga gttaacaaca aacacaatga
      601 agaaaacgaa atcacaatta aaaacactca ggataataat ttgtgagagt accccgatat
      661 cgcatattcg ttatagcgat atcttaaact accggaatga actgctgcat ggagaaacgc
      721 tttacctaga taatccaaga tccaacaaaa aaggaagaac cgtgcgcaca gttgataact
      781 atatcgccct gctctgttcg ttgttacgtt ttgcgtatca gtcgggattt atatcaacca
      841 aaccatttga aggagtaaaa aaattacagc gaaacagaat aaagcctgac ccgttatcta
      901 aaacagaatt caatgcatta atggaaagtg aaaaaggaca gagccaaaac ttgtggaaat
      961 ttgccgttta ctccgggctt cgtcacgggg aactggcagc tctggcgtgg gaggatgtgg
     1021 atttcgagaa gggaa
//
LOCUS       DAESHI010000303         1023 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.306, whole genome shotgun sequence.
ACCESSION   DAESHI010000303 DAESHI010000000
VERSION     DAESHI010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.306"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(77..865)
                     /locus_tag="KW011_004521"
     CDS             complement(77..865)
                     /locus_tag="KW011_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBH7662658.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(905..>1023)
                     /locus_tag="KW011_004522"
     CDS             complement(905..>1023)
                     /locus_tag="KW011_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBH7662659.1"
                     /translation="KEAIRFASAVAAMKCTQPGGRAGIPNREQTESFLSLYA"
BASE COUNT          255 a          263 c          239 g          266 t
ORIGIN      
        1 atcgctggca ggtttttttt gcttatggaa aacaaaatcc gctgaaattc ccagcggatt
       61 ttccgaaatt tcaatttcag gatacttctt gctcggcaac caccagttca acattctctt
      121 cacttaacaa ctggcagaaa gaattgggag gcggggcatc agtaaaaaaa gccctgacat
      181 tccgtgcatt gccaattgaa acggcagcag acgcggcaaa cttcgtatga tcggccacta
      241 acaatgtatt ccgcgcatgt ttaatcatcg ttctcgcgac taacgcttca tttacatcaa
      301 attccagtag ggtgccgtca tgttcaatgg cacctatact ggtgattaaa taatctgcgc
      361 ggaagccttc aataaaatcc acggctcctg ggccgataat cccgccgtta tgagcgcgta
      421 aagtgcctcc cggcaccatc acttcaatat cctgattctt ataaagaatc tgtgccacac
      481 gtaggctgtt agtgataatg cgtaaatcac gccggttgag taatgccctg gcaacggctt
      541 ctacggttgt accgatggtg ataaagacgg tacagcgttc gggaaggtaa tcggcgactg
      601 cctcagcgat cgcccgtttc tcggcggtga gcgaaagttc ccgttgctca aaagcggtat
      661 tcatgacgct ggagacgcgc ccggcccctc catgatgacg ggtgatcaag ccctgctcac
      721 tgagttttcg gatatcccgg cgcacggttt gcgttgaaac atccagcagt tgtgccagtt
      781 cttcaatatt catataaccg cgttctgcga ttagatgaac gagttggtca tggcgtggat
      841 tacctgtcac ttcgatgata ctcatgaata ctcccttgcc cctcctcggt agatgtttac
      901 cattttacgc ataaagtgac aaaaatgatt cggtttgctc acgattagga atgccggccc
      961 gaccgccagg ctgggtacat ttcatcgcgg caaccgcact ggcaaaacga atagcctctt
     1021 ttg
//
LOCUS       DAESHI010000304          996 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.307, whole genome shotgun sequence.
ACCESSION   DAESHI010000304 DAESHI010000000
VERSION     DAESHI010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.307"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..228)
                     /locus_tag="KW011_004523"
     CDS             complement(<1..228)
                     /locus_tag="KW011_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HBH7662660.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASS"
     gene            385..561
                     /locus_tag="KW011_004524"
     CDS             385..561
                     /locus_tag="KW011_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HBH7662661.1"
                     /translation="MLNLPIRKLFPTRIASSYGMNFEFMPELKWSFGYPGAIIFMILA
                     GLTPYLYFKRKNWL"
BASE COUNT          326 a          167 c          189 g          314 t
ORIGIN      
        1 acttgaggca ctaaaagcaa cattaataga acctgggcca gggaaatggt atttaataag
       61 tctgtaagga tcaacttttg ctgagatatc gcttaggccg agtgagagaa agtcttgttc
      121 taccatatca ccatattgca tatcatcagc agaaaaatca tcgaataaac taatagtttc
      181 gaaaagtgta cagggaatat taattggcgg aagtaatatg ttattcattt gcatttgcct
      241 tggttatatt taagctcaag atcaattcga gaacaaatgc tagggtaaaa aaatatttaa
      301 aaaagaagta cacttcacat tttttcatta catctctgaa gagaacctcg ttaatgattg
      361 gtcttgctca ttgttaataa caaaatgttg aatttaccga ttaggaaact ttttcctacg
      421 cggattgcct ccagctatgg catgaacttt gagtttatgc cagaactgaa gtggagcttt
      481 ggctaccctg gcgcgattat ctttatgatc cttgcgggcc tgacaccgta tctgtacttt
      541 aagcgtaaga actggctgta actatacgat gtcgctgtga gtaagttagt gtgctgacag
      601 cgacatttta gccgagggaa tcgcagataa cataattgtt gtgaataata tccatgccac
      661 gttgagttgg acgcttaatg actcacatct caccccttca attgctgcag agataataga
      721 aaaaagtgtc aaacccaatg aaatcaatac aaagtagatt gccttacatc cagtatctct
      781 tattcttttt gctccaatat tgatatttac ataggtatta ttaataacca gaataaaact
      841 caaagccagg ttgatatcat tcaaagttat taatattgac catgatgtta atgtatagat
      901 tatgaactga aagcgtgata aacgctcaag cctggatggg tagaatatat ttctgatgga
      961 tgatttgaac atttttacat tgttttatat atataa
//
LOCUS       DAESHI010000305          985 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.308, whole genome shotgun sequence.
ACCESSION   DAESHI010000305 DAESHI010000000
VERSION     DAESHI010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.308"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            229..>985
                     /gene="gspA"
                     /locus_tag="KW011_004525"
     CDS             229..>985
                     /gene="gspA"
                     /locus_tag="KW011_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HBH7662662.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTSRVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIAITC"
BASE COUNT          280 a          199 c          237 g          269 t
ORIGIN      
        1 catctttatt atgattcgcc agatggaagg ggaaacgtag tgtgggcacg atgtatgttc
       61 taataaaata gattgggtgg gttgtatatt gacgttagta taaatagtat taatgccgct
      121 tgcatgaatc aagcaaaatt tatttatatt agtaatatgt atttaattaa taaatacata
      181 cattattttt atgattaagg ataatcaata attaaaacag gaagttctat gtctacgaga
      241 agagaagtta ttctttcctg gttgcgtgag aaacgtcaga cctggcgtct gtgctatttg
      301 ttgggtgagg ctggaagtgg aaaaacctgg ctggcgcagc aactgcaaaa agataaacat
      361 cgccgtgtga ttactttaag cctcgttgtt tcctggcaag gtaaggccgc atggatcgtt
      421 accgacgata acgcggctga acagggctgc cgtgacagcg cctggacgcg agatgagatg
      481 gcggggcaat tactgcatgc gcttcatcga actgatagtc gctgccctct tattattatt
      541 gaaaatgctc acctgaatca ccgccgcata cttgatgatc tccaacgcgc gatatctctt
      601 attcctgacg gtcaattttt gctgatcggc agacccgatc gaaaagtcga acgtgatttt
      661 aaaaaacagg gcattgaact tgtctcgaca ggacgtctga cggagcacga acttaaggca
      721 agcatccttg aaggtcagaa tattgaccaa cccgatctcc ttttaacctc cagagttctg
      781 aaacggatag ctttattatg tcggggcgat cgcagaaagc tggcgcttgc cggcgaaaca
      841 attcgcttat tacagcaagc cgagcagacc agcgttttca ctgcaaaaca atggcgaatg
      901 atttaccgca tattgggtga taaccgcccc cggaaaatgc agcttgccgt ggtaatgagt
      961 ggaaccatta tcgctattac ctgtg
//
LOCUS       DAESHI010000306          960 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.309, whole genome shotgun sequence.
ACCESSION   DAESHI010000306 DAESHI010000000
VERSION     DAESHI010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.309"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..>960
                     /gene="htrE"
                     /locus_tag="KW011_004526"
     CDS             <1..>960
                     /gene="htrE"
                     /locus_tag="KW011_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HBH7662663.1"
                     /translation="WASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDDSVYLS
                     FTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARVSYSVNTGYTM
                     NKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFVLHSGGLTFSNDS
                     FSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRIALDINDLENDVE
                     LKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADIYDEQGNVIGNVG
                     QGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQ"
BASE COUNT          278 a          194 c          238 g          250 t
ORIGIN      
        1 tgggcttccg gacaaaatcg tagcaattac tctattggct acagtaacag tgcatcctgg
       61 ggcagctaca gtgtcagtgc ccagcgttca tggaatgaag acggcgacac tgacgatagc
      121 gtttatctca gtttcaccat tcctattgaa aaattacttg gcactgaaca acggacttca
      181 ggtttccaga gtattgatac tcaaatgagc agtgacttta agggtaataa ccaactcaac
      241 gtcagtagca gtggctatag tgataacgct cgcgtcagtt atagcgtaaa tactggctat
      301 acgatgaata aagccagcaa agatttgagt tatgttgggg gttatgccag ctatgaatca
      361 ccgtggggaa ccctggcagg ttcagtttct gcaaatagcg ataacagtcg tcaagtgtct
      421 ctcagcaccg acggtggttt tgtattgcat agcggcggcc tgaccttcag taatgacagt
      481 tttagcgact ccgatacact ggcggtagtt caggctccag gtgctcaagg agcgcgaata
      541 aattatggca acagtactat cgatcgatgg ggttatggtg tcaccagcgc tctttctcct
      601 tatcatgaaa accgtatcgc gctggatatc aacgatcttg agaacgatgt tgaattaaaa
      661 agtaccagtg cagtagctgt accgcgtcag ggttcagtcg tctttgctga ttttgaaacc
      721 gtgcaagggc aatcagccat tatgaacatc acacgaagtg atggtaaaaa tattccattt
      781 gctgcagata tttatgatga gcaaggcaat gtcattggta atgttggaca gggtggacaa
      841 gcatttgttc gtggcattga gcagcaggga aatatcagca ttaaatggct cgaagaaagt
      901 aaacccgtaa gttgtcttgc gcattatcaa caaagttcag aagcagaaaa aatagcgcaa
//
LOCUS       DAESHI010000307          959 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.310, whole genome shotgun sequence.
ACCESSION   DAESHI010000307 DAESHI010000000
VERSION     DAESHI010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.310"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            1..959
                     /locus_tag="KW011_004527"
                     /pseudo
     CDS             1..959
                     /locus_tag="KW011_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          194 a          319 c          251 g          195 t
ORIGIN      
        1 attacccaac gacaaaaaca aactcaggtc gctaatatct gacctgaaac aacatggtca
       61 gatactgctg gttgttgatc agccagctac catcggtgcg ttacctgtcg ccgttgcccg
      121 ctcagaagga gtccttgtcg gatacctccc tggactggcc atgcgccgca tagccgactt
      181 acacgccggt gaagctaaaa ctgatgctcg tgacgctgcc atcattgccg aagctgcccg
      241 taccctgcct cacgcgctac gcacgctgaa actggctgac gagcaaatcg ccgaactctc
      301 catgctctgc ggcttcgatg atgatcttgc cgcacagaca acgcaggcca gcaaccgtat
      361 ccgcggcctt ctgacccaga tacatccggc actggagcgc gttctcggtc cgagacttga
      421 gcacccggcg gtactcgatc ttctccagcg atatccctca ccagaaaaac tcgcttcgct
      481 gggcttcgct gggtgagaag aagctggcag cccagctctg caaacttgcg cctcgtctgg
      541 gtaaacgcct tgcagcagac atagctcagg cactggccga acaaaccgtc gtcgttcccg
      601 gcacgaatgc cgctgccgta gtactgccac gtctggcact ccagctcatc acgctgcgta
      661 agcaaagaga cgaggtggcg cttgaggtag aacagcgagt tcttgctcac cctctttacc
      721 cggtcctgac cagtatgccc ggagtcggtg tcaggaccgc agccagactc ctcaccgagg
      781 tcgcctgccg cgccttcgcc tctgccgcac atctcgctgc ttatgctggc cttgcgccgg
      841 taactcggcg atccggctcg tcaatacgcg gtgagcatcc ctcgcgacgg ggtaataaag
      901 ctctcaaacg ggcgttgttc ctgtcggcct tcgccgcgct cagggatccg ctctccagg
//
LOCUS       DAESHI010000308          934 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.311, whole genome shotgun sequence.
ACCESSION   DAESHI010000308 DAESHI010000000
VERSION     DAESHI010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.311"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(196..>934)
                     /locus_tag="KW011_004528"
     CDS             complement(196..>934)
                     /locus_tag="KW011_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBH7662664.1"
                     /translation="GPTLTFLFVSPLLNPIIVGLMWMTFGWKVTLLYAIIAAGVSVLA
                     SIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIENPVVTCC
                     STKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPF
                     AIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVILLKSMF
                     RMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          279 a          198 c          199 g          258 t
ORIGIN      
        1 tgcttctccg ataaaatgtc agagaagcat gattacgtgg ttattgtctg ttgatgactt
       61 gttgaccgag ccgtcatcat tctcaatgca ttagttaaat catttgtgat ttttaaggat
      121 tatcctgtct atgattttct acttagaagg atatggcact aactatgcat cttatagttt
      181 actgctggca tctatttaaa atagcaattg agtcaaatag cccatcaaga tagccatacc
      241 taatataacc gtcaggaatg cagctatcat cggcattctg aacattgatt taagtaatat
      301 cacttccgtc aggcttgcgc cggcactgcc gatgattaat gccattaatg ctcccagacc
      361 cattcctttt gtcatcagaa cagatgccag agggataacc gcctcagccc ggatatacag
      421 cggaataccg acaacggcgc ttaatgggat ggcgaaggga ttatctgctc ctgcatgagc
      481 ggcaatccac gctgagggaa taaagccata aataaaagat cctattaata ctcctaacaa
      541 aagatatggc agtacatctt taaactgttg caacgcatct ttggtggcta gttttatggg
      601 attaatactt actacagctt tggtactgca acaggtaact acggggttct caatggcttt
      661 agccgctgga ctacaacaac tcacttttat gggcgtatat gtcgttcccg gcgaagtttt
      721 tgctggagca caacaatttg ctgatgagct tttactggca atgatatgac gttcaaaccc
      781 cagagaatcc aggataatac tggcaagaac ggagacgccg gctgcgataa tcgcgtacaa
      841 caaggtgact ttccaaccaa aggtcatcca cattagtccg acgataatgg gattaagtaa
      901 tggtgaaaca aataaaaaag tgagagtcgg acca
//
LOCUS       DAESHI010000309          930 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.312, whole genome shotgun sequence.
ACCESSION   DAESHI010000309 DAESHI010000000
VERSION     DAESHI010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.312"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..>930)
                     /locus_tag="KW011_004529"
     CDS             complement(<1..>930)
                     /locus_tag="KW011_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBH7662665.1"
                     /translation="VPGADEVLPPEPPAYRVLTGVVDGFGRTLAFHRAAEGDVAGAVT
                     GVMDGAGRRFHLVLTTQAQRAEEARKPHTASLSSPDSPCPLSAPSFPDTLPAGTEYGA
                     DNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGELRAVYDRSGMQVRGFTYDAEHA
                     GRMVAHHYAGRPESCYRYDDTGRVTEQVNPEGLDYRFEYGESRVIITDSLNRREVLYT
                     EGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRRTEYRLHMASGKLTSVILP
                     DGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKG"
BASE COUNT          141 a          364 c          254 g          171 t
ORIGIN      
        1 cccttctcat catactcccg gctgctgcga agcccgtccg ggtacgtcac cgacgtcacc
       61 tgccgctggc tgttatagcc ataccgtacc gtcctgccgt ccggcagtat cactgaagtc
      121 agctttccag acgccatatg cagtcggtac tccgtccgcc gtcccgccgc atccgtctgc
      181 gccttcagcc tccccgcctc atcatactcg ctgcgggtga tgctcccgtc ggcatgctcc
      241 ttcttcacca cccgcttcag gccaccctcg ccttccgtgt acagcacctc ccgccggttc
      301 aggctgtccg tgatgattac ccggctctct ccgtactcaa agcggtagtc cagcccctcc
      361 gggttgacct gctccgtcac ccgaccggta tcatcatacc ggtagcagct ctccggcctc
      421 cccgcataat ggtgcgccac catccgcccg gcgtgctccg catcataagt aaacccgcgc
      481 acctgcatcc cgctgcggtc atacaccgcc cgcagttcac caccggccgt gtacgtgtag
      541 cgcgccagcg gcgcagtcgg ctgttcatcc gggtatgccg ggtcgtgtgt cagccatacc
      601 gcctccagcc ggataccgtt atcggcaccg tactctgtac cggcgggcag cgtgtcgggg
      661 aaggaaggag ctgagagagg gcagggactg tcaggggaag ataacgaagc ggtgtgcggt
      721 ttacgggcct cttccgcccg ctgcgcctgt gtggtcagca ccaggtgaaa acggcggccc
      781 gcgccgtcca tcacccccgt caccgcgccc gccacgtcac cctccgctgc ccggtgaaag
      841 gccagcgtcc gcccgaagcc gtccaccacg cccgtcagca cccggtatgc gggcggctcc
      901 ggcggcagca cctcgtccgc ccccggcacc
//
LOCUS       DAESHI010000310          906 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.313, whole genome shotgun sequence.
ACCESSION   DAESHI010000310 DAESHI010000000
VERSION     DAESHI010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.313"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="KW011_004530"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="KW011_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HBH7662666.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITYIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          267 a          144 c          222 g          273 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tatgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 tgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtga
//
LOCUS       DAESHI010000311          905 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.314, whole genome shotgun sequence.
ACCESSION   DAESHI010000311 DAESHI010000000
VERSION     DAESHI010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.314"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..796
                     /locus_tag="KW011_004531"
     CDS             <1..796
                     /locus_tag="KW011_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH7662667.1"
                     /translation="HDNETELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVAS
                     DVNDPATTCTCALYGFQFQGAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQS
                     KNSQTSSLWDAFAYTLAILYYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITL
                     EQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGY
                     ENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPVSLAG"
BASE COUNT          259 a          182 c          197 g          267 t
ORIGIN      
        1 tcatgacaat gaaaccgagt tgatttacgt caagaaaggg gtagcaaggt ttaccatcga
       61 ttcttcgtta tatgtcgcgc acgccgatga cattgtggtg atagaacgtg gcaggctgca
      121 tgcggtggcc tctgacgtta acgatccagc aacgacgtgt acctgtgcgc tgtacggctt
      181 tcagtttcag ggggctgagg aaaatcagct actgcaaccg cattcttgcc cggtaattgc
      241 cgcagggcag ggaaaagaag tcattaaaac cttatttaat gagctaagtg tgattttgcc
      301 gcaaagtaaa aatagccaaa catcttcgtt atgggatgca tttgcctata cattagcaat
      361 tctttactac gaaaacttta aaaatgctta tcgttcggag caggggtata ttaaaaaaga
      421 tgttctgata aaagatattc ttttctatct gaataataat tatcgcgaaa aaatcacttt
      481 agaacagtta tcgaaaaaat ttcgtgccag cgtcagttat atttgccatg aatttaccaa
      541 agagtatcgt atttccccta ttaactatgt tattcaacgg cgtatgacgg aagcaaaatg
      601 gtcactcact aatactgaat tatcacaggc agagatctcc tggcgtgtgg gttatgaaaa
      661 tgtcgatcac tttgccaaac tgtttttgcg ccatgtcggt tgttcgccca gcgattaccg
      721 taggcaattt aaaaattgtt ttgcggaaca agaaatctta tctgaatttc ctcaaccggt
      781 aagtcttgcc ggataaatct ggcggcgcac tttgttaaaa agctgcgccg cctgcagatt
      841 atttgttctg atagtctttc aggatctgcc gacctgctac gtaaatcata atttcgtctg
      901 taccg
//
LOCUS       DAESHI010000312          897 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.315, whole genome shotgun sequence.
ACCESSION   DAESHI010000312 DAESHI010000000
VERSION     DAESHI010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.315"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..>897)
                     /locus_tag="KW011_004532"
     CDS             complement(<1..>897)
                     /locus_tag="KW011_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HBH7662668.1"
                     /translation="PAYRVLTVVVDGFGRSLTFHRAAEGDVAGAVTGVTDGAGRRFHM
                     ALSTQAQRAEASRKQRASSLSSPASPRSVSSSQVFPDTLPAGTEYGADNGIRLEAVWL
                     THDPAYPDEQPTAPLARYTYTAGGELRAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGR
                     PESRYRYDDTGRVTEQVNPEGLDYRFEYGESRVIITDSLNRREVLYTEGEGGLKRVVK
                     KEHADGSITRSEYDEAGRLKAQTDAAGRRTEYRLHMASGKLTSVVLPDGRTVRYGYNN
                     QLQLTSVTYPDGLRSSRKYDRQG"
BASE COUNT          149 a          337 c          243 g          168 t
ORIGIN      
        1 ccctgacggt catatttccg gctgctgcgt aaaccatccg gatatgtcac tgacgtcagt
       61 tgcaactggt tgttgtaacc ataccgtacc gtcctgccgt ccggcagtac cactgaagtc
      121 agctttccag acgccatatg cagccggtac tccgtccgcc gccccgccgc atccgtctgc
      181 gccttcagcc tccccgcctc atcatactcg ctgcgggtga tgctcccgtc ggcatgctcc
      241 ttcttcacca cccgcttcag gccaccctcg ccttccgtgt acagcacctc ccgccggttc
      301 aggctgtccg tgatgattac ccggctctct ccgtactcaa agcggtagtc cagcccctcc
      361 gggttgacct gctccgtcac ccgaccggta tcatcatacc ggtagcggct ctccggcctc
      421 cccgcataat ggtgcgccac catccgcccg gcgtgctccg catcataagt aaacccgcga
      481 acctgcgtcc cgctgcggtc atacaccgcc cgcagttcac caccggccgt gtacgtgtag
      541 cgcgccagcg gcgcagtcgg ctgttcatcc gggtatgccg ggtcgtgtgt cagccatacc
      601 gcctccagcc ggataccgtt atcggcaccg tactctgtac cggcgggcag cgtgtcgggg
      661 aaaacctgag aagaggaaac agaacgggga ctggccgggg aagataagga agaggcgcgc
      721 tgtttacggg acgcttccgc ccgctgcgcc tgcgtgctca gcgccatgtg aaaacggcgg
      781 cccgcgccgt ccgtcacccc cgtcaccgcg cccgccacgt caccctccgc cgcccggtgg
      841 aaagtcagtg agcgcccgaa gccatccacc acgaccgtca gtacccggta tgcgggc
//
LOCUS       DAESHI010000313          877 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.316, whole genome shotgun sequence.
ACCESSION   DAESHI010000313 DAESHI010000000
VERSION     DAESHI010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.316"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..>877
                     /gene="cadA"
                     /locus_tag="KW011_004533"
     CDS             <1..>877
                     /gene="cadA"
                     /locus_tag="KW011_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HBH7662669.1"
                     /translation="NFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVK
                     GDVNEETFNEAYMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEI
                     KRLRTESDGWFFDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPG
                     MEKDGTMSNFGIPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTD
                     FKRAFDLNLRVKNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVL
                     PTMVMTPYAAFQKELH"
BASE COUNT          238 a          221 c          212 g          206 t
ORIGIN      
        1 caacttctca ccgatttacg aaggtaaatg cggtatgagc ggtggccgtg tagaagggaa
       61 agtgatttac gaaacccagt ccactcacaa actgctggcg gcgttctctc aggcttccat
      121 gatccacgtt aaaggtgacg taaacgaaga aacctttaac gaagcctaca tgatgcacac
      181 caccacttct cctcactacg gtatcgtggc gtccactgaa accgctgcgg cgatgatgaa
      241 aggcaatgca ggtaaacgtt tgatcaacgg ttccattgaa cgtgcgatca aattccgtaa
      301 agagatcaaa cgtctgagaa cggaatctga tggctggttc tttgatgtat ggcagccgga
      361 tcatatcgat acgactgaat gctggccgct gcgttctgac agcacctggc acggcttcaa
      421 aaacatcgat aacgagcaca tgtatcttga cccgatcaaa gtcaccctgc tgactccggg
      481 gatggaaaaa gacggcacca tgagcaactt tggtattccg gccagcatcg tggcgaaata
      541 cctcgacgaa catggcatcg ttgttgagaa aaccggtccg tataacctgc tgttcctgtt
      601 cagcatcggt atcgataaga ccaaagcact gagcctgctg cgtgctctga ctgacttcaa
      661 acgtgcgttc gacctgaacc tgcgtgtgaa aaacatgctg ccgtctctgt atcgtgaaga
      721 tcctgaattc tatgaaaaca tgcgtattca ggaactggct cagaatatcc acaaactgat
      781 tgttcaccac aatctgccgg atctgatgta tcgcgcattt gaagtgctgc cgactatggt
      841 aatgactccg tatgctgcgt tccagaaaga gctgcac
//
LOCUS       DAESHI010000314          846 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.317, whole genome shotgun sequence.
ACCESSION   DAESHI010000314 DAESHI010000000
VERSION     DAESHI010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.317"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..545
                     /locus_tag="KW011_004534"
     CDS             <1..545
                     /locus_tag="KW011_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662670.1"
                     /translation="IQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQVEKVGIIRALTV
                     GVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFSVGKTMKENTGQ
                     TAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAPVINWNCGATQP
                     VPDAPVPKDLPPGMPDMRQF"
     gene            621..>846
                     /locus_tag="KW011_004535"
     CDS             621..>846
                     /locus_tag="KW011_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662671.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGI
                     TYANPVNPVNPVLGAKVLPGETDLALPCPLP"
BASE COUNT          209 a          184 c          279 g          174 t
ORIGIN      
        1 agatccagaa cattggcgtt aatcagatac agacggttgg tgtcaaccag gtggaaacgg
       61 tgggcagtaa ccagattatc aaagtgggat caaaccaggt tgaaaaggtg gggatcattc
      121 gtgcgctgac ggtgggtgtg gcttaccaga cgacggtagg cggcattatg aatacctcgg
      181 tggcgttgtt gcagtcctca caggtagggc tgcataaatc actgatggtg ggaatgggct
      241 acagcgtcaa tgtagggaat aacgtcacct tctcggtggg caagacgatg aaggaaaaca
      301 ccggacaaac agcagtttat tctgccggtg agcatcttga actctgctgt ggtaaggcaa
      361 ggctggtgct gacgaaggac ggaagcatat ttctcaacgg tacgcacatt catctggaag
      421 gggagtcgga tgtgaacggt gatgcgccag tgattaactg gaactgtggt gccacacaac
      481 ctgtaccgga tgcgcctgtg ccgaaagatt taccccccgg aatgccggat atgcggcaat
      541 tttgagtcag gtgcaaaaaa ttaaaacgga actggtgcag tgaccagatg aaccatatta
      601 aaagcagaag gaattgattc atgggcggcg gaaaaccggc ggcgcggcag ggcgacatga
      661 cccgcaaggg actggatatt gtgcagggtt cagcgggtgt gctgataggt gcgccgacgg
      721 gagtggcctg ttcggtgtgt ccgggaggga ttacctatgc taacccggtg aacccggtga
      781 acccggtgct gggtgcgaag gtgcttccgg gcgagacgga ccttgcgctg ccctgcccgc
      841 tgccgt
//
LOCUS       DAESHI010000315          815 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.318, whole genome shotgun sequence.
ACCESSION   DAESHI010000315 DAESHI010000000
VERSION     DAESHI010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.318"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(246..>815)
                     /locus_tag="KW011_004536"
     CDS             complement(246..>815)
                     /locus_tag="KW011_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH7662672.1"
                     /translation="FFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIV
                     IAGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFF
                     IAVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQ
                     IHLLDKYRKVPPEPVHADIPVGAVSDVKA"
BASE COUNT          259 a          203 c          174 g          179 t
ORIGIN      
        1 agggacagct gatagaaaca gaagccactg gagcacctca aaaacaccat catacactaa
       61 atcagtaagt tggcagcatc acccgctttg gcttgataac cggataacaa cgtgcgcttt
      121 gttgatgccg gatgcggcgt gaattcctta tccggtctac aaaatttgca aatccaaagc
      181 attacattaa gacgtggcct gataaacata tcgcatcagg ccattttcgt tagctattgt
      241 ccacgtcagg ctttcacatc actcactgca ccgaccggaa tatcagcatg aacaggctcg
      301 ggcggtactt tgcgatattt atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca
      361 ttgaggcgat agaaacggaa gtagagaatc agcgttacca tgaaaaacag agttggtaga
      421 gcaatcataa taaactgcat acctaatagg gcttgcgtag actgttcaac attcggtaca
      481 tagccaatca tccctaacac aaccgcaatg aaaaaagccg caaaggctga accgcccttc
      541 accaccatag tctgcacgga gtaagcgata ctttcacagc gtacgtgcag tttatattca
      601 ccgtaatcaa cggtatctgc caccatgatg acctgtaata cccagaaaag cgccgttccc
      661 acattcagca aaatacccgc aatcacaatg aggacgacgt tgtgatagct cattaatgcc
      721 attaacagga gaacaccaca gcttaacacc ggaagaatag atgctccggc ccataaaatg
      781 cgtcgggata atgatttaac taagcgtggg aagaa
//
LOCUS       DAESHI010000316          803 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.319, whole genome shotgun sequence.
ACCESSION   DAESHI010000316 DAESHI010000000
VERSION     DAESHI010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.319"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..494)
                     /locus_tag="KW011_004537"
     CDS             complement(<1..494)
                     /locus_tag="KW011_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662673.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDGK
                     IYENAWWGFSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDA"
BASE COUNT          265 a          169 c          145 g          224 t
ORIGIN      
        1 ttcgcatcac ccggacagtt agaagaggct acccaccaag catttttata taccgcacca
       61 ttaaagacga cataccaggt ctgaccgccc tgctgtttat tccaggcaac tacagaagaa
      121 ttagacggaa cagtgccctg tgctggtgta gccgaaccgc cattagccgg cgtattggat
      181 gcaggcgtat ttgaatttga agaagatgag ctgccgctct tttcgcagga aagtgtattg
      241 ccaaactgac taatttcagc agctgttgcg gtacgcttta aacgccacgg gttagttgca
      301 tcatttgctt tcgcttttcc cgggcaattt gtagaaaaac cccaccaggc attttcataa
      361 attttgccat cgaaaataac ctgatattta ttaccacctt gttggttatt ccatgcttcc
      421 attgccaatg ctggcagagc ggaacacacc agccccatac caatcataga tttagtaaat
      481 atatttaatt tcattataat cccttgtgac gtaaaaactg caaaacaaaa gtcctacgca
      541 ggaattattt acgttttacg agaacccttc aatatgaatt aaagtgaggc attaaaaata
      601 agaaaaaagc ctgacaaatg aagaatttta aaaacagaaa cattcatatt taaaatatta
      661 aattgaattg atattttaaa tatgaataat ttattcgttc tgacagtacg aataagatat
      721 gcagtcaaca aatgcaaaaa gggcgccgaa gcgccctttt caattcaaaa ctaattaacg
      781 tgtaattagc ccagaacttt agc
//
LOCUS       DAESHI010000317          800 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.320, whole genome shotgun sequence.
ACCESSION   DAESHI010000317 DAESHI010000000
VERSION     DAESHI010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.320"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            2..757
                     /gene="istB"
                     /locus_tag="KW011_004538"
     CDS             2..757
                     /gene="istB"
                     /locus_tag="KW011_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039272515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HBH7662674.1"
                     /translation="MSDNLLNKLTQLKLPAMAGSLIRQRETPQTYDELSFEERLTLLV
                     DDELLSRENSRVARLRKNACLKYQATPEGLRYPASRGLRAEQMRELLNGHYIIHRKNL
                     LITGPTGCGKSWIANALGEQACRQKYSVRYCRTGRLLEQLAQGRVDGSWLKYLKQLQK
                     IQVLILDDLGLEQLSNAQCNDLLEITEDRYGQSSTIVVSQFPVDKWHGLMENPTTADA
                     ILDRLVHNSHRVVLQGESLRKNPPTVESSEKTS"
BASE COUNT          247 a          175 c          227 g          151 t
ORIGIN      
        1 tatgagtgat aatctgttaa acaaactgac ccaactgaaa ctcccggcga tggccgggag
       61 cctgatacgg caacgggaaa cgcctcagac ttacgacgaa ctgtcgttcg aggaaaggct
      121 gacactgctg gttgatgacg agcttctgag cagagaaaac agtcgtgtgg cacggttgag
      181 aaaaaatgcc tgcctgaaat atcaggcaac accggaaggg ctgcgttatc cggcctcaag
      241 aggtctgcgg gctgaacaga tgagggaact gctgaacggc cactacataa tccacaggaa
      301 aaacctactg ataaccggtc cgacaggatg cggtaagagc tggatagcca atgcgctggg
      361 cgaacaggca tgtcgacaga aatacagcgt ccggtactgt agaaccgggc gattactgga
      421 acagttggct cagggacgtg ttgatggcag ttggctgaaa tatctgaaac agttgcaaaa
      481 aatacaggtt ctaatcctgg acgatctggg gctggaacag ttaagtaacg cccagtgtaa
      541 tgaccttctg gagatcacag aggatcgtta cggacaaagc agcacgatag tggtaagcca
      601 gttcccggta gataaatggc atggtctgat ggagaaccca acaacagcag atgccatcct
      661 ggacagactg gtacataact cacacagagt ggtccttcag ggagagtccc tgaggaaaaa
      721 tccccctaca gtggaaagca gcgaaaaaac gagttaaaaa gcggtagaaa aagttaagtt
      781 cccggttgat cgaatggatg
//
LOCUS       DAESHI010000318          799 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.321, whole genome shotgun sequence.
ACCESSION   DAESHI010000318 DAESHI010000000
VERSION     DAESHI010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.321"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..741)
                     /locus_tag="KW011_004539"
     CDS             complement(<1..741)
                     /locus_tag="KW011_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HBH7662675.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLC"
BASE COUNT          268 a          137 c          161 g          233 t
ORIGIN      
        1 acataaacag gtaaagacca taaatatggt gaaagtaaaa aagaaggaaa atacagcaat
       61 ggaaaaaatt ttatcggaga acaggaataa atctaaaaga tagattgctg aaatacagac
      121 tccactttta catgcgcgac aaaaattact gccttgatga aaaaaccaga aagggaagaa
      181 catcactgca aatacccaaa aaatagcaaa gtaataatga tcactggaag acgctgattc
      241 actttctgaa ggattagtcg gatattggtg ttgtgaagtg agttggttat tcaaagcgtt
      301 tatagctagc tctaatccct gagctaattt ttgttgttta aatgcgggta tcatgttgct
      361 acggatgata tctccagcca gagcatcggt taccttttct tccagcccat aacctacctg
      421 gatgcggaca gtacgatctg accaggcaac aatgatcagt atcccatcat tacgtttggc
      481 atctcctaaa cgccaattgt caaaaactct tgtagcatat tgttcaattg tctcgtcttt
      541 ggttgtggga acaattaata cagcaatttg cacttgggta ttattgaaaa tagattctaa
      601 cttgtgcgtc agttctttct tttcttgtga gtttaagcta ctcgtaagat ccgtaacttg
      661 ttgctgtaat tgtgggacaa tagttggttg ggctgcaaca aaggaacaca cgaaagtgaa
      721 cattagtatg aaatagcgca ttataaattt ctcattaaat ctggataaat aaattgacgg
      781 cggcatcggt aattttaga
//
LOCUS       DAESHI010000319          790 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.322, whole genome shotgun sequence.
ACCESSION   DAESHI010000319 DAESHI010000000
VERSION     DAESHI010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.322"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..>790
                     /locus_tag="KW011_004540"
     CDS             <1..>790
                     /locus_tag="KW011_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HBH7662676.1"
                     /translation="ECQIRNSNFDNCYLYASHFTRAEFLSAKEISFIKSNLTAVMFDH
                     VRMSTGNFKDCITEQLELTIDYSDIFGNEDLDGYINNIIKMIDTLPDNAMILKSVLAV
                     KLVMQLKILNIVNKNFIENMKKTFSHCPYIKDPIIRSYIHSGEDDKFDNFMRQHRFSK
                     VNFDTQQMIHFINRFNMNKGLIDKNNNFFIQLIDQALRSTDDMIKANAWYLYKEWIRS
                     DDVSPIFIETEEKLRTFNTNKLTRNDNIFILFSSVDDGPVMVVSS"
BASE COUNT          281 a          115 c          134 g          260 t
ORIGIN      
        1 cgaatgtcag attagaaatt caaacttcga taattgttat ctttacgctt cgcacttcac
       61 cagagcagag tttctgtctg ccaaagaaat atcatttatt aaatcgaatt tgacagctgt
      121 tatgtttgat catgtgcgaa tgtcgacagg gaattttaaa gattgcatta cagaacaatt
      181 ggaattaact attgattatt cagatatatt tgggaatgaa gatctcgatg gttatatcaa
      241 taacattata aaaatgattg atacattgcc agataatgca atgatattga aatccgttct
      301 ggcagtaaaa ctggtgatgc aattaaaaat tctcaatatt gttaataaaa actttattga
      361 gaatatgaaa aaaacattta gccattgtcc ttatataaaa gatccaatta tacgtagtta
      421 tatacattct ggggaagatg acaagttcga taattttatg cgtcaacatc gattcagtaa
      481 ggtgaatttc gatacccaac agatgatcca ttttattaac agatttaata tgaataaagg
      541 gctgattgat aaaaataaca atttttttat ccaacttatc gatcaggcct tacgatcaac
      601 ggatgatatg atcaaagcaa atgcctggta tctttataaa gagtggattc gtagtgatga
      661 tgtttcacct atatttatag aaactgaaga aaagttaaga acctttaaca ctaataaatt
      721 aacacgaaac gataatatat ttatcctgtt ctcctctgtc gatgatgggc ctgttatggt
      781 ggtaagctcc
//
LOCUS       DAESHI010000320          789 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.323, whole genome shotgun sequence.
ACCESSION   DAESHI010000320 DAESHI010000000
VERSION     DAESHI010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.323"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..458)
                     /locus_tag="KW011_004541"
     CDS             complement(<1..458)
                     /locus_tag="KW011_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HBH7662677.1"
                     /translation="MAHIQLIKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHFYPDGSRGRRAKSIA"
     gene            complement(530..742)
                     /locus_tag="KW011_004542"
     CDS             complement(530..742)
                     /locus_tag="KW011_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662678.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETKKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          203 a          193 c          173 g          220 t
ORIGIN      
        1 gcgatggatt ttgcccgacg cccgcggcta ccgtccggat aaaaatgttc ggtgtaaaat
       61 ccagcctgaa tggttaccca ctcgcgaaaa gcctcaaacg actttagcaa tgccgtatcc
      121 cgggttctgc gtgtcgcaac tgtattcaga tattgctctg cggcttcgct cagagctggc
      181 gtatgttccc gacctactga ttcgcacagg taatcaacga aaccagacag cagttctcgt
      241 tcgcgaggcg tgatcgcccc accgaccgga gtccagtaat cgaatcccag ttgcaggagt
      301 ttgaaaaaac gcttgtggaa tgcgtagtta cgcacacgct taaagtctgc gtgtatccac
      361 tcacctgttt tgatttgatg cagaaaatcg caactctccg gcgtcgccgg gagaagtaat
      421 ccggaagagg tttgtttgat cagttgtata tgcgccatcg tagttctccg ctggcgcagt
      481 agaatgggtg ttcagcccgt tatgtagtat accagaatta atgccaatac taacaggatg
      541 ctctgactcg caattcatcc agcagtttat catttcccat aatgtcactt accctcatcg
      601 gtaaaaaaat tgcctttcga ccattacgat acatcattga ttttttggtt tcagggaagt
      661 aatccatttc gactataact gacaggtcat cacgacgtat gactgcgtat ttactactaa
      721 atagtttctt tattttttcc acgatgcctc caggtttata agtacaaacg gttatatcaa
      781 catagagac
//
LOCUS       DAESHI010000321          779 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.324, whole genome shotgun sequence.
ACCESSION   DAESHI010000321 DAESHI010000000
VERSION     DAESHI010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.324"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            43..303
                     /locus_tag="KW011_004543"
     CDS             43..303
                     /locus_tag="KW011_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662679.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
     gene            300..>779
                     /locus_tag="KW011_004544"
     CDS             300..>779
                     /locus_tag="KW011_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662680.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     "
BASE COUNT          174 a          217 c          244 g          144 t
ORIGIN      
        1 tgatttttac ctgtcagaac attatcagat ggaacgtgca ggatggatca ggaaatgacg
       61 ttttcactca gctatgaaca actgacccgg tttgcggaaa aacgcatccg ggaatgcaat
      121 ctggacagcc acggcgtaac ttatctctgc gagtcagcca aagccggagc cgtgctgatc
      181 ttctggcatg aactggccat aaatggctat acctcaatga atgccataaa gagacaggag
      241 atcattgacg ccgaccatca gcgcctcaga aagctcatct ggccggagga tgactggaaa
      301 tgactcagag ccgccgaccg tcgccactgc agcggagagt gctgattgtg ctggctgccc
      361 ttgatgaaaa acggccagga cccgtgctga cgcgggacat agaacgggtg ctggagcaaa
      421 gcggggaagc cccggtctac gggccaaacc tgcgcgcctc ctgccgtcgt ctggaggatg
      481 ccggctggct gcgtaccctg cgcgctccga acctgcagct ggcagtggag ctgacggacg
      541 ccggccgggc cgtggcacag cctctgctgc tggcagaaca ggaccgcatc cgggcagaac
      601 agcgtgcggc agaagtcgtc gtactgccgc tggtcccggc agcaggtctg cccgcaggcg
      661 gcacttctgc cactgatctg gcggtggagc tgaacggcat aacgtatcag gcctgccgtg
      721 gggattttgt ggtgcgtctc gacggcagca cctgtctgca gttatggaat aaagaaggc
//
LOCUS       DAESHI010000322          775 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.325, whole genome shotgun sequence.
ACCESSION   DAESHI010000322 DAESHI010000000
VERSION     DAESHI010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.325"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..343
                     /locus_tag="KW011_004545"
     CDS             <1..343
                     /locus_tag="KW011_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HBH7662681.1"
                     /translation="ADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVAHGELGGDFSLV
                     PDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPSLIVIA
                     DKAAAAELALG"
     gene            complement(405..>775)
                     /locus_tag="KW011_004546"
     CDS             complement(405..>775)
                     /locus_tag="KW011_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HBH7662682.1"
                     /translation="QDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELVERTPGYHGW
                     QQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLAKCLQKGGDC
                     QGYLFRCLHCGKLRLWGDFS"
BASE COUNT          210 a          190 c          175 g          200 t
ORIGIN      
        1 tgcagatggt catttttgtg gcaatttgcc gaatacgacc catttccatg agcaaaccgt
       61 ggagttcccg atccagggag agatggttga tattgtggcg catggtgagt tgggcggcga
      121 cttttcgtta gtgcctgaca gctacgtgac gatggggccg aaaagcatca tggcggcgaa
      181 aaacttgctg ataattgtca gtggcgcagg taaagcgcag gcactgaaaa atgtgctgca
      241 aggtccggtt accgaagatg ttccagcttc cgtgttgcag ctgcatcctt cgctgatagt
      301 aatcgctgat aaagccgctg ccgcagaatt agcgctcggt taagctgaag aaataaggcc
      361 ggatgtatga attacgctcc ggcctgttct cattatttaa ataactacga aaaatcaccc
      421 cacagtctca gcttgccgca gtggagacag cggaagagat aaccctgaca atcaccaccc
      481 ttttgcaggc atttagctag atcagaattt ctaataccga aattctcaca atcttcttca
      541 aggttggcaa attcatcgag gcgatctttt atatcattcc agcccacata gccgataaaa
      601 gcacagaaat cgccacaatg cgcaagccag aattcctgtt gccatccatg ataacctggc
      661 gtgcgttcaa ccaactcttt cagcatttca tcaggatatg tgttcttaat accggcaaat
      721 tcgtcctctt catcatactc aaattcaaca ccttctatgc tggcatcatc ctgaa
//
LOCUS       DAESHI010000323          772 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.326, whole genome shotgun sequence.
ACCESSION   DAESHI010000323 DAESHI010000000
VERSION     DAESHI010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.326"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..431)
                     /locus_tag="KW011_004547"
     CDS             complement(<1..431)
                     /locus_tag="KW011_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH7662683.1"
                     /translation="MVKSMKTNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIR"
     gene            488..757
                     /locus_tag="KW011_004548"
                     /pseudo
     CDS             488..757
                     /locus_tag="KW011_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          228 a          146 c          176 g          222 t
ORIGIN      
        1 ttgcgtatta cacagctttt tgtagatata tttccaggca ggcagtgaag aaggtaaaac
       61 atggaaatgg aatcaaattg accatgtaac gccgcgggat aaggttcaaa aacatcatgg
      121 ctaattttat gtttaatttt tgattcccca gcccttgtag atgccgcgtt caggctagct
      181 tcgttcaaat ccattaaaga tatcagacta ctctcaggta cgtgagtaag gtaaaaccca
      241 gttccaacac caatatccag atggttgtta cctaaatgtt ccagaaagtg tggaagaagg
      301 tgttcctttg taggacatcc ccatgcaagc cgatttgata ctcccaaaac ccaccagtca
      361 taaagcttta gggtaagtgg tgtgtaaatt ttagccccat catctgtgtt tgttttcatt
      421 gatttcacca tgttatagtt ttatttgtga attaaatcaa ttatggcgat gaattacaag
      481 gggttaaatg ctgccgcagc atagcgatat tgaaatagcc tagtatgctt cgatacagca
      541 ggagccgaat ggctggaaga ccgtcaccac acagttctac atccaggaat ttagtgagta
      601 tattgcgcca ctgcaggatg ctgtagatct ggaaatcgca acggaggaag aaagatcgtt
      661 gctggaggca tggaataaat atcgggtatt gttgaatcgt gttgatacat caactgcacc
      721 tgatattgag tggcctgcaa atcctgtcag ggagtaatca ttgggattat gc
//
LOCUS       DAESHI010000324          771 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.327, whole genome shotgun sequence.
ACCESSION   DAESHI010000324 DAESHI010000000
VERSION     DAESHI010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.327"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..372)
                     /locus_tag="KW011_004549"
     CDS             complement(<1..372)
                     /locus_tag="KW011_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662684.1"
                     /translation="MHMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAW
                     RAEQEAKPFTFEWNGRIWNAGP"
     gene            complement(323..>771)
                     /locus_tag="KW011_004550"
     CDS             complement(323..>771)
                     /locus_tag="KW011_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH7662685.1"
                     /translation="YRAKSLTFYGINGPIYHTRYCYWPISRLTGWVKINITTEDIIYR
                     IVASSVRNRWGDPDIGGLIIAAYQGEADGDKVIRLVRGQSYRGSRLGPVGISVPSTPT
                     GTYIASPQFFITGCSEHSLPGSYCALSGVPDAHVSGAMPGLFIRTS"
BASE COUNT          169 a          211 c          191 g          200 t
ORIGIN      
        1 gggaccagca ttccagatac gaccgttcca ttcaaacgtg aacggcttcg cctcctgttc
       61 tgcgcgccat gcttcaattt cctgacgcct ggcctctctg gccgcttcca gcatttctgg
      121 tgtcacagtg aatggggcta tctcacccca tttgccactt tgcagttcct gccagatttg
      181 ctgacccgtc ggtgcgacat catcagcggt ggctgtgtag gggactgcct ggtccctgtc
      241 gtcaaaaaaa acgtcacagt ctactgcgcc actttcggta taacggggat taatgatttt
      301 tttaatttcc acggtgcatt cctcacgatg tgcgaataaa aagcccgggc attgcgccag
      361 agacatgtgc atccggcacc ccggacaggg cgcaatatga ccccggtaat gaatgctctg
      421 aacatcccgt aatgaaaaat tgtggggatg ctatatacgt tccggtggga gtactgggca
      481 ctgaaatccc caccggtccc agtcgtgagc ctctgtatga ctgccccctg acaagtctga
      541 tgactttatc accgtcagct tctccctggt acgcagcaat aatcagcccg ccaatgtcag
      601 ggtctcccca tctgttgcgg acagagctcg ccacgattct gtaaataata tcttctgtgg
      661 ttatatttat tttcacccag ccagtcagtc tggatatggg ccagtagcag tagcgggtgt
      721 gataaatggg gccgttaatg ccgtaaaaag taagggattt ggctctgtac c
//
LOCUS       DAESHI010000325          765 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.328, whole genome shotgun sequence.
ACCESSION   DAESHI010000325 DAESHI010000000
VERSION     DAESHI010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.328"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(405..765)
                     /locus_tag="KW011_004551"
                     /pseudo
     CDS             complement(405..765)
                     /locus_tag="KW011_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          176 a          193 c          189 g          207 t
ORIGIN      
        1 ggagggacag ctgatagaaa cagaagccac tggagcacct caaaaacacc atcatacact
       61 aaatcagtaa gttggcagca tcaccgtaat tgagttgttg accaatttgt ctgtggcagg
      121 taatcacttg ctgtctttgt ctccatccct ggatactggg gatcatcctg cggcatacaa
      181 gtcagattgg gttttaccca cttcctgcaa tacagtgtgt aatcgtaagg gcttaatgct
      241 ctgaagaatg ttttgaaaac attaggcgca ctactggcct ggtcaatagg atatggcagt
      301 cttaattgtt tcgctatgtc tggcaactta cttggatcgg gcgtaggcgg tttgctgggt
      361 ttcgctccat atccgccatt atttaagtgt ccatatttag atgccagccc cattgaatct
      421 atatactgaa ccggattcaa cggatactga taaaaattcc atcccccctt cagtccaatc
      481 ggatcctgag tgatataccg cccctgcagc gggtcataat agcggtggcg gttgtaatac
      541 aggccggact cctcatcata ctgctgcccc ggcaggcgaa tgagctgctg cagatgatgc
      601 gggttctcat cactcagcag gttgccccat tcatcatatt ctgcgcacca cgctgttgtc
      661 ccttccgtgc tgataagggc cagcggcagg ccgcgatggt cgcagtggta caggtgaatt
      721 tttcgcgccg gcgtgtatac cgggtccatc tggctttgca tctgc
//
LOCUS       DAESHI010000326          753 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.329, whole genome shotgun sequence.
ACCESSION   DAESHI010000326 DAESHI010000000
VERSION     DAESHI010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.329"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..696)
                     /locus_tag="KW011_004552"
     CDS             complement(<1..696)
                     /locus_tag="KW011_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBH7662686.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAG"
BASE COUNT          177 a          210 c          179 g          187 t
ORIGIN      
        1 gcccgcgcgc cacgccatag caatgccatc gccagaagaa atatccggat tggtggtgta
       61 ctgatacacc ttcgacgcac cgccggttgc cagtaccact gcttttgcgt ggcaggtttc
      121 caccgtttct ttatttcggt tccataccca cgcgcctaca acccgtcgcg tgcccggcag
      181 gccaattttg tcagagacaa tcagatcaac tgcgttgctg cgctccagca cacgaatatt
      241 cggatggttc tgcgccttgc tcaccagcgt ggattgtact tctctaccgg tggcgtcggc
      301 ggcatgaaga atacgacggt gactatgtcc accttcacgg gtcagatggt agctttcttc
      361 gccattcggt tgaacgtggg tatcaaacaa aaccccctgg tcaattaacc attgcacaca
      421 ggaacgagca ttgctggcga caaattcaac tgcatggcga tcgcaaatac cagccccggc
      481 aatcaatgtg tcttccacat gcgagtcaat gctgtcagtt tcatcaaaca cggcggcaat
      541 accgccctgg gcataaaatg ttgaaccttc cgttaccggg cctttactta gaacgatgac
      601 ctgatgctgg tcagccaggc gtagcgccag tgaaagtccg gctgcgccgc taccaataat
      661 caacacgtca catgaatgtt cagggagagt attcattttc tttgtttaat ttactaaaca
      721 ttgtttggtc agcatagcat catgttgtgc gaa
//
LOCUS       DAESHI010000327          751 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.330, whole genome shotgun sequence.
ACCESSION   DAESHI010000327 DAESHI010000000
VERSION     DAESHI010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.330"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            584..>751
                     /locus_tag="KW011_004553"
     CDS             584..>751
                     /locus_tag="KW011_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HBH7662687.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVID"
BASE COUNT          213 a          135 c          164 g          239 t
ORIGIN      
        1 cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact tcatatttga
       61 acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat cgtaaaggaa
      121 accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt cgatagcgcg
      181 agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat tattcaatac
      241 aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt ctctaaaaaa
      301 ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg tcagggatta
      361 aaagatgaac gtaaatttat ttaacgcatt aattttaaag ggttttattg tttgttgacg
      421 aaaacaggaa tcgtgttcgg tctcttttta tctgttaaaa gccagaagca tttccttcgc
      481 tgactttata gtcaaccata acacacactc tactgtctga gtccaacgtt ttttaacatt
      541 cttgttaaaa ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac agcctgcgcc
      601 cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg gcgatataca
      661 cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc cgtggcgaga
      721 tttacttatc tcagtgggag acgttatcga t
//
LOCUS       DAESHI010000328          748 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.331, whole genome shotgun sequence.
ACCESSION   DAESHI010000328 DAESHI010000000
VERSION     DAESHI010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.331"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..402
                     /locus_tag="KW011_004554"
     CDS             <1..402
                     /locus_tag="KW011_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HBH7662688.1"
                     /translation="TDVINAIVKPFYERQMSKSDNPIDKVVRSKVEPTDQALTGDMNS
                     LAAVLWAMVQGKSKDVPQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQT
                     VLDGKVIDERTTSHINRMQEDTLISSAYPEE"
BASE COUNT          262 a          139 c          141 g          206 t
ORIGIN      
        1 actgatgtga ttaatgccat tgtcaaaccg ttctatgaac gtcagatgag taagtcagat
       61 aatccaattg ataaagttgt aaggtcaaag gtggaaccca cagaccaagc attgactggt
      121 gacatgaact ctttagccgc tgttttatgg gctatggttc agggcaaatc aaaagatgtt
      181 ccacagtttt cacctgaagc attaaataat ctgaaaggtc gagtggaaac attcacacaa
      241 gatttaaagg ttatcaaacc gtcagtaaat attcagcctc agacaatcaa tatcacaact
      301 cagacggtac ttgatggaaa ggttattgat gaaagaacca catcgcatat taatcgtatg
      361 caggaagata ccttgattag ctcagcttat ccagaggaat aaacaatgtt agagctacta
      421 atatttattg ccacacatgg caatctatta atctttatct agtatgacga ttcaactatg
      481 aatagtcgaa tgctacgtga aatcttctga acagatacca acatcaaaaa ttagatttca
      541 cgtagctcac gcttaacaga ataggaataa caacaatgtt tgacacaaag caaattagat
      601 actcaggtaa agatggtatc tattttcacc ttagagataa tgtggaatct tttcttgcct
      661 taacagctac tgaacagatg gactatgact caccgatgat ggtgacaact caggctatgc
      721 aatcggggca aactatcact gataatgt
//
LOCUS       DAESHI010000329          748 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.332, whole genome shotgun sequence.
ACCESSION   DAESHI010000329 DAESHI010000000
VERSION     DAESHI010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.332"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..407
                     /locus_tag="KW011_004555"
     CDS             <1..407
                     /locus_tag="KW011_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBH7662689.1"
                     /translation="WRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYT
                     QRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELAILTWVDWY
                     NNRRLLERLGHTPPAEAEKAYYASIGNDDLAA"
     gene            449..649
                     /locus_tag="KW011_004556"
                     /pseudo
     CDS             449..649
                     /locus_tag="KW011_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          216 a          175 c          198 g          159 t
ORIGIN      
        1 ggtggcgggt ctcatcgtcc atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tatgggcccg tcgaccgtcc ggcacggtcc atcacagtga taaaggttct cagtatgtat
      121 cgctggccta cacacagcgg cttaaggaag ccggattact ggcatcaaca ggaagtacag
      181 gcgactcgta tgacaacgcg atggcggaga gcatcaatgg tctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccgtgcag aagtggaact ggccatactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tactcctccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaacg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcaa ccttcacgat aaaaaacttt tcattaaagg
      481 taaagcggat caatatccgg cacttcattc tctgatatct ccgacccgca ttaatctgaa
      541 agaaatagaa attcactggc gtgaagtgct acgtctggcg acctcaataa aacagggaac
      601 agtgacagca tccctgatgc tgagaaaact tgccagttac cccaaacaaa agtaaggcag
      661 tatgactttc cgtgagacag atgctggcca acgtgggata tttaacgcgg gttaacatcg
      721 ttgtcagagc cgcatgggtg agctgttg
//
LOCUS       DAESHI010000330          744 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.333, whole genome shotgun sequence.
ACCESSION   DAESHI010000330 DAESHI010000000
VERSION     DAESHI010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.333"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..397)
                     /locus_tag="KW011_004557"
     CDS             complement(<1..397)
                     /locus_tag="KW011_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBH7662690.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRL"
BASE COUNT          233 a          174 c          108 g          229 t
ORIGIN      
        1 ccaacctgga ccatgctaaa ttcacaccgt gctgcgcggc ctgattaaat agtgcatacg
       61 ctttggcata atccacccgc acctgctcac cgtcgtagta gatacccccc agcgctaacg
      121 gagcttctct gtcaccttgt ttagccgcta actcgaacca tttaatcgct tcctgaacat
      181 caggtttggt atcataacga ggctgataat attcccagcc taacaggctc tgcgcgtcga
      241 tatctcccga ttcagccatt ttatgcagtg tatttataac atcgggagca ggtttaccac
      301 cgtatatgct gtcatacaat tcatgcgctt tctcgctggc caccacgctt ggtgaaacta
      361 tcagcattac agaaaataat attcttttag ttaacatgta tacctttatg tcctcactgt
      421 catatatttc cataataatt aaccaccaaa tatccattta atgaataaat acagtgccat
      481 aaaggtccaa gtaataagca cacatttagc ctgaaacatt atcatccatg tcaccaaatc
      541 gtttatttag tgagataaaa ctagcatatt cgtaagataa aaaatgcaca tggcaatcaa
      601 gaaatgatat tattactttt ttgatgtaaa actatttagt tttaaaattc attttttcaa
      661 catatagagc attcatttta tgaatgcaaa aaaacatacc tgacacacaa attactctcc
      721 tctgtaatat tttctcatta acac
//
LOCUS       DAESHI010000331          736 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.334, whole genome shotgun sequence.
ACCESSION   DAESHI010000331 DAESHI010000000
VERSION     DAESHI010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.334"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(1..736)
                     /locus_tag="KW011_004558"
                     /pseudo
     CDS             complement(1..736)
                     /locus_tag="KW011_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
BASE COUNT          195 a          195 c          158 g          188 t
ORIGIN      
        1 caaaaaggca aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc
       61 aatatatccc gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt
      121 ccagtcccat tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc
      181 tggataagca ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt
      241 gatggtcagt gcttgtttcg ctgcctgctg tgtccactcc tgccaatttt tggccttcat
      301 ctgatgagtc caggccagca aagacgccac ctctttacca tcccatgcgc gggatttagc
      361 gtaagccgtt tgtgtcgtct ggtcagtttg gagaatgttg ccatgcaccg tacgccagac
      421 agtaaaggtc tctgcctgac catttttcac cgtaatggtt tcctcacggc ttaacatttt
      481 cacccactta ccattatgca agtagtagcc tggtttctct gccgacagcc gttcagcaaa
      541 aatatcgaca tcatcgccga aacctgccgt tgatccccag gaaatcacac cattatgacc
      601 aaaaaccagc ccaggatagg ataggcaaat ggtgtattgc cagtgacatc ataaccagca
      661 ccgtgcagac caataccata agtatacgca ggcgcatacc agccaaactg cggaccattt
      721 accatgattg ctttcg
//
LOCUS       DAESHI010000332          713 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.335, whole genome shotgun sequence.
ACCESSION   DAESHI010000332 DAESHI010000000
VERSION     DAESHI010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.335"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(76..423)
                     /gene="tnpB"
                     /locus_tag="KW011_004559"
     CDS             complement(76..423)
                     /gene="tnpB"
                     /locus_tag="KW011_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBH7662691.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(420..>713)
                     /locus_tag="KW011_004560"
     CDS             complement(420..>713)
                     /locus_tag="KW011_004560"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH7662692.1"
                     /translation="EPGISISKLALENGINANLLFKWRQQWREGKLLLPSSESPQLLP
                     VTLDAAAEQPESLAEDPKTLSISSEVTFRHGTLRFNGNVSEKLLTLLIQELKR"
BASE COUNT          157 a          188 c          198 g          170 t
ORIGIN      
        1 atgtcgtcag aagagatgtc gttcataagc ccgtatttta ccgggcttat tctgtgacaa
       61 ccaggataaa gagatttaca gcatggtcag ggaggtcagc agccgcttag gctgtcgcca
      121 gtcgatacct tccagcagca tcgccagctg cgcctgtgta aggaacactt tgccatcacg
      181 ggctgacggc caggcgaagc gcccacgctc cagccgtttg gtcaggaggc acagtccgtc
      241 actggtggac cacagcagtt taacctgact gccgctgcgg ccccggaaaa tgaaaacatg
      301 gccggacatg ggatcgtctt tcagcgccgt ctgtactttc gcagccaggc cgttgaagcc
      361 atttctcata tcggtgatac cggcaaccag ccaaattttg atcccggaag gtaacgggat
      421 catcgcttca gttcctgtat cagcagagtc aggagctttt cgctgacatt gccattgaag
      481 cggagcgtcc cgtgccggaa cgttacctca ctgctgatac tgagggtttt cgggtcctct
      541 gcgagcgatt ctggctgttc ggcagctgca tcgagagtca caggaagtag ctgggggctc
      601 tctgaagaag gtaatagcag ctttccctcg cgccattgtt gtcgccattt gaacaacaga
      661 ttggcgttaa tgccattttc aagagcaagt tttgagatgg atatcccggg ttc
//
LOCUS       DAESHI010000333          707 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.336, whole genome shotgun sequence.
ACCESSION   DAESHI010000333 DAESHI010000000
VERSION     DAESHI010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.336"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            282..686
                     /gene="stpA"
                     /locus_tag="KW011_004561"
     CDS             282..686
                     /gene="stpA"
                     /locus_tag="KW011_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HBH7662693.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT          213 a          140 c          158 g          196 t
ORIGIN      
        1 tcttttattc tgttatattg catttttatt cacttcggtt ttgtcaggct tgcggaatta
       61 gcgagcagag agcgccgttc tgttcaccaa taatctaaat tactcatcaa aatggctaat
      121 atataatctt gaaattattc tgagagtttc agaaaataac agctgaaata atctcgcgca
      181 agactgtaaa tagattaaat tttgtggaaa tataataagt gatcgcttac actacgcgac
      241 gaaatacttt tttgttttgg cgttaaaagg ttttctttat tatgtccgta atgttacaaa
      301 gtttaaataa cattcgcacc ctccgtgcga tggctcgcga attctccatt gacgttcttg
      361 aagaaatgct cgaaaaattc agggttgtca ctaaagaaag acgtgaagaa gaagaacagc
      421 agcagcgtga actggctgag cgtcaggaaa aaattagcac ctggctggag ctgatgaagg
      481 ctgacggaat taacccggaa gagttattgg gtaatagctc tgctgctgca ccacgcgctg
      541 gtaaaaaacg ccagccgcgt ccggcgaaat ataaattcac cgatgttaac ggtgaaacta
      601 aaacctggac cggtcagggc cgtacaccga agccaattgc tcaggcgctg gcagaaggta
      661 aatctctcga cgatttcctg atctaattac tgaggccgga tgtcgct
//
LOCUS       DAESHI010000334          698 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.337, whole genome shotgun sequence.
ACCESSION   DAESHI010000334 DAESHI010000000
VERSION     DAESHI010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.337"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..>698
                     /locus_tag="KW011_004562"
     CDS             <1..>698
                     /locus_tag="KW011_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HBH7662694.1"
                     /translation="SRHTDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAI
                     NAKRVYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLR
                     VTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCY
                     RANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLA
                     EAFEHYNEWHPHSA"
BASE COUNT          172 a          169 c          213 g          144 t
ORIGIN      
        1 cagtcgtcac actgatgata cggatgtgct tctccgtata caccatgtta tcggagagct
       61 gcccacgtat ggttatcgtc gggtatgggc gctgcttcgc agacaggcag aacttgatgg
      121 tatgcctgcg atcaatgcca aacgtgttta ccggatcatg cgccagaatg cgctgttgct
      181 tgagcgaaaa cctgctgtac cgccatcgaa acgggcacat acaggcagag tggccgtgaa
      241 agaaagcaat cagcgatggt gctctgacgg gttcgagttc tgctgtgata acggagagag
      301 actgcgtgtc acgttcgcgc tggactgctg tgatcgtgag gcactgcact gggcggtgac
      361 taccggcggc ttcaacagtg aaacagtaca ggacgtcatg ctgggagcgg tggaacgccg
      421 cttcggcaac gatcttccgt cgtctccagt ggagtggctg acggataatg gttcatgcta
      481 ccgggctaat gaaacacgcc agttcgcccg gatgttggga cttgaaccga agaacacggc
      541 ggtgcggagt ccggagagta acggaatagc agagagcttc gtgaaaacga taaagcgtga
      601 ctacatcagt atcatgccca aaccagacgg gttaacggca gcaaagaacc ttgcagaggc
      661 gttcgagcat tataacgaat ggcatccgca tagtgcgc
//
LOCUS       DAESHI010000335          697 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.338, whole genome shotgun sequence.
ACCESSION   DAESHI010000335 DAESHI010000000
VERSION     DAESHI010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.338"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..>697
                     /locus_tag="KW011_004563"
     CDS             <1..>697
                     /locus_tag="KW011_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662695.1"
                     /translation="AQQKAKTEKQNQRAQAAAARAQARQQQQIAARKEKAELKLLDVS
                     SSISAMHRLSVAEQYKAIAQAREIAKQYEQGAISLARMNSQMKRLQQQQRKINGNRKA
                     QFAPVKGGSGNSATMGALLFGGATAAAGVMAVSKTSEFMANSFANAETEGELIQRARL
                     GGVDYNLLNNLERWAYDNGIDSLQGDPGRRKILDAIGKDVKERATKSYDEAEYVVDKK
                     TGKGIWKGGDSGI"
BASE COUNT          237 a          138 c          182 g          140 t
ORIGIN      
        1 ttgctcaaca gaaagctaaa acagaaaagc agaatcaaag agcacaagct gccgcagcaa
       61 gagcacaggc aaggcaacag caacaaatag cagcacgtaa agagaaagca gaattaaaac
      121 tccttgatgt atcaagctct atcagtgcaa tgcaccgtct ctctgtagcg gaacaataca
      181 aagctattgc acaggctaga gagatagcaa aacaatatga gcaaggggct atcagcctgg
      241 cgcgtatgaa tagtcagatg aaacgcctac aacagcaaca acgtaagatt aatggcaaca
      301 gaaaagctca atttgccccc gttaagggag gatcagggaa ttccgccacg atgggcgctc
      361 tgttatttgg aggcgctacg gctgctgctg gcgtgatggc tgttagtaaa acctctgaat
      421 ttatggctaa cagctttgct aatgctgaaa cagaagggga gctaatccaa cgtgcaaggc
      481 ttggtggagt tgattacaac ttacttaata atcttgagcg ttgggcgtat gacaacggga
      541 tcgatagcct ccagggtgat ccgggacgca gaaaaatact agatgccatc ggtaaagatg
      601 taaaggaacg cgctaccaaa agctatgacg aggctgaata tgtagttgat aagaaaacag
      661 ggaaaggcat atggaagggc ggtgattctg gtattaa
//
LOCUS       DAESHI010000336          695 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.339, whole genome shotgun sequence.
ACCESSION   DAESHI010000336 DAESHI010000000
VERSION     DAESHI010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.339"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..>695
                     /gene="tuf"
                     /locus_tag="KW011_004564"
     CDS             <1..>695
                     /gene="tuf"
                     /locus_tag="KW011_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBH7662696.1"
                     /translation="VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLG
                     RQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEG
                     DAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKV
                     GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG
                     TIKPHTKFESEVY"
BASE COUNT          162 a          167 c          205 g          161 t
ORIGIN      
        1 gtagactgcc cggggcacgc cgactatgtt aaaaacatga tcaccggtgc tgctcagatg
       61 gacggcgcga tcctggtagt tgctgcgact gacggcccga tgccgcagac tcgtgagcac
      121 atcctgctgg gtcgtcaggt aggcgttccg tacatcatcg tgttcctgaa caaatgcgac
      181 atggttgatg acgaagagct gctggaactg gttgaaatgg aagttcgtga acttctgtct
      241 cagtacgact tcccgggcga cgacactccg atcgttcgtg gttctgctct gaaagcgctg
      301 gaaggcgacg cagagtggga agcgaaaatc ctggaactgg ctggcttcct ggattcttac
      361 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
      421 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      481 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      541 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      601 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      661 aagccgcaca ccaagttcga atctgaagtg tacat
//
LOCUS       DAESHI010000337          690 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.340, whole genome shotgun sequence.
ACCESSION   DAESHI010000337 DAESHI010000000
VERSION     DAESHI010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.340"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
BASE COUNT          241 a          105 c          143 g          201 t
ORIGIN      
        1 agtctatttt ataaaaaaat taagtaggct aagaagtacc ttagaaatca attttaaggg
       61 gtctaaaacg ctctgtaacg agctaaactg tataagtggc ataagatgcc ttttagactg
      121 acactcgctt agagagcttc ctagagcttc tgggaggatt cattagatat cgttgtatac
      181 agagacacag tatctataaa gggtattaag agattcttag aagttactgt aaagaaccac
      241 taagataaga agagagggac aaagaaccga aggaaagtcc cttattaagt aggagtctta
      301 atagactgtc ttaatagata acttaaaagt cttaaaggat atcttccaag aagagaggaa
      361 cgaagaacca gaggaagtga ccgttgaccg tttgaacaga ctgtttaagt aaagcctata
      421 aagtatctat taattaaggg ctatgtctca atactagcag tttgtactag cagtttgtca
      481 atacctttgt ctttagcaat tcgtgctatt tattaaatat agctgttaaa acagagtatg
      541 ctttttaacc taaaaccttg tcgcttcgct ctgggtcaca tgttcttggg ttcttagtga
      601 gagttacact atcttctaag gtactcaata aaaagacgag taacaaccga agaacgagga
      661 tagaggtatt gtaataactt agaaggtgtc
//
LOCUS       DAESHI010000338          683 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.341, whole genome shotgun sequence.
ACCESSION   DAESHI010000338 DAESHI010000000
VERSION     DAESHI010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.341"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..560
                     /locus_tag="KW011_004565"
     CDS             <1..560
                     /locus_tag="KW011_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HBH7662697.1"
                     /translation="RFSQLESALNTQKNSIPALEKEVKALDKQMVAAQKAADAYWGKD
                     ANGKQMTREEAFKKIHQQRDEFNKQNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVP
                     IQQKRDFDINEQRRQTFLQSQKLSRKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMK
                     IDDINQANERWKKDTEQLRRNGVIK"
BASE COUNT          244 a          121 c          165 g          153 t
ORIGIN      
        1 atcgctttag tcagcttgaa tctgcgttga atacccagaa gaactcgatt cctgcgctgg
       61 aaaaggaagt gaaagcgctt gataagcaaa tggtggcggc gcagaaagca gcggatgcat
      121 attggggaaa agatgcaaac ggtaagcaaa tgacccgcga agaagccttt aaaaaaattc
      181 accaacagcg tgatgagttt aataaacaga acgatagcga ggcgttcgcg gttaaatatg
      241 acaaagaggt ttatcaaccg gcgattgcgg catgtcataa acagagtgaa gagtgttatg
      301 aagtgccgat tcagcagaaa cgagatttcg atatcaacga acaacggcga cagacttttc
      361 tgcaatcaca aaaactcagc cgtaaattgc aggatgactg ggtaacgctc gaaaaagggc
      421 aatatccgtt aacgatgaaa gtttcagaaa taaacagtaa aaaagtcgcg attttgatga
      481 aaattgacga tatcaaccaa gctaatgaac gctggaaaaa agataccgaa caactgcggc
      541 gcaatggggt gattaagtaa ctcatgttaa gtgcctttgc cggataatat aaaaagttat
      601 ccggcaaatt attttttaca ttagccctag tagctgtgga atgaacaagg atatttgcgg
      661 aatgtaagtg atcatcatca acg
//
LOCUS       DAESHI010000339          661 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.342, whole genome shotgun sequence.
ACCESSION   DAESHI010000339 DAESHI010000000
VERSION     DAESHI010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.342"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..>661
                     /locus_tag="KW011_004566"
     CDS             <1..>661
                     /locus_tag="KW011_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBH7662698.1"
                     /translation="TFIADDNNAELIPCLSTDLLVSLGIKKIALLDNKEHSAEKHVPD
                     NSACTPLQDRLADASTEFDVGQQHLSLSVPQIYVGRMARGYVSPDLWEEGINAGLLNY
                     SFNGNSINNRSNHNAGKSNYAYLNLQSGINIGSWRLRDNSTWSYNSGSSNSSDSNKWQ
                     HINTSAERDIIPLRSRLTVGDSYTDGDIFDSVNFRGLKINSTEAMLPDRQHGFAPVIH
                     GI"
BASE COUNT          201 a          128 c          144 g          188 t
ORIGIN      
        1 tacttttatt gcagatgata ataacgcaga actcatccca tgcctgagta cagatcttct
       61 tgtcagtctt ggtataaaaa aaatagcgtt attagacaat aaagaacatt cggcagaaaa
      121 acatgtgccg gacaacagcg catgcacacc acttcaggat cgtttggccg acgcatcaac
      181 tgagtttgat gttggtcagc aacatctgtc actctccgtt ccgcaaattt atgttggcag
      241 aatggctcgc ggctatgttt ctcctgatct gtgggaagaa ggaataaatg cagggcttct
      301 aaactatagt tttaatggta attctattaa taatcgtagt aaccataatg caggaaaatc
      361 aaactatgca tatttgaatt tacagagtgg catcaacatt ggtagttggc gactacgcga
      421 taactcaacg tggagttata acagtgggag tagcaattca tctgacagca ataaatggca
      481 gcatatcaat acgtcggctg aacgtgacat tattccctta cgctcacggt taacggtagg
      541 tgatagttat accgatggcg atatttttga tagtgtgaac tttcgtggcc tcaaaataaa
      601 ttcaacagaa gcgatgttgc ccgatagaca acatggtttc gctccggtga ttcatggtat
      661 t
//
LOCUS       DAESHI010000340          659 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.343, whole genome shotgun sequence.
ACCESSION   DAESHI010000340 DAESHI010000000
VERSION     DAESHI010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.343"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..216
                     /locus_tag="KW011_004567"
     CDS             <1..216
                     /locus_tag="KW011_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBH7662699.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTRVQGTVVMFSASGKK"
     gene            complement(271..369)
                     /locus_tag="KW011_004568"
                     /pseudo
     CDS             complement(271..369)
                     /locus_tag="KW011_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(415..597)
                     /locus_tag="KW011_004569"
     CDS             complement(415..597)
                     /locus_tag="KW011_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH7662700.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGYEQIFEDKLSGTRTGRPG
                     LKRALKRLQKGDALVV"
BASE COUNT          189 a          166 c          129 g          175 t
ORIGIN      
        1 gatggaactc tatacggagc cacaattctt ggcgatggag ttgtgcacaa tagcttcggt
       61 caaaaactga tgcgcattta caatcagaaa ggcatctttt caaacacaaa agattccgaa
      121 gaaggcttaa cacatatact ctcagagcat ttcgagaatg ttaaaaccag ggttcaaggt
      181 actgtagtaa tgttttccgc ttcagggaaa aaatagcatc caaccgcagc acgttcttgc
      241 ttaagacgtg ctgcggcata atcccaatga ttactccctg acagggttcg taggccactc
      301 aatatcaggt gcagttgatg tatcaacacg gttcagcaac acccgatact ttttctaggc
      361 ttccaccacc agcacgacaa gatgccgcat acagtgaccc agtcagtcca gttttcagac
      421 aaccagtgcg tcaccttttt gaaggcgctt taaagcacgt tttaatccag gtcggcctgt
      481 ccttgttccg cttaatttat cttcaaatat ttgttcatat cctgcacaaa caagagcgtt
      541 tcgttgcagg tctgtattct ggtcatttgt tgataccctt acatagccaa tcagcacgct
      601 gaatctcccg tccaaaagca caaatcatgc catgcaggcc agaaaccgcc attatctaa
//
LOCUS       DAESHI010000341          651 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.344, whole genome shotgun sequence.
ACCESSION   DAESHI010000341 DAESHI010000000
VERSION     DAESHI010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.344"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(88..615)
                     /locus_tag="KW011_004570"
                     /pseudo
     CDS             complement(88..615)
                     /locus_tag="KW011_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105657.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
BASE COUNT          161 a          188 c          148 g          154 t
ORIGIN      
        1 tttctttttt tcattggagc acctgtgttg ttctgaggtg agcatatcac ctctgttcag
       61 gtggccaaat tcagtgtgcc acttcagcca ttcttcttcc gagagcttga cgacatccgc
      121 cagttgtagc gcctgccgca aacacaagcg gagcaaatgc tcgtcttgcc atagatcttc
      181 acgaatatta ggatcgaagc tgacaaaacc tccggcatgc cggatcgccg tcatcgcagt
      241 aaatgcgctg gtacgcgaag gctcggcaga caacgcaatt gaacagagat gtaaccattc
      301 gccatgtcgc cagcagggca agtctgtcgt ctctaaaaaa agatcggcac tggggcggac
      361 cataaacgta aatgaacgtt ccccttgatc gttcagatcg acaagcaccg tggatgtccg
      421 gtgccattca tcttgcttca gatacgtgat atcgactccc tcagttagca gcgttctttg
      481 cattaacgcc ccaaaaggat catcaccgac ccgacctata aatccactta ttccgcctaa
      541 tctggcgatt cccaccgcaa cgttagctgg cgcgccgcca ggacaaggca gtaggcgccc
      601 gtctgattct ggcaagagat ctacgaccgc atcccctaaa acccatactt t
//
LOCUS       DAESHI010000342          650 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.345, whole genome shotgun sequence.
ACCESSION   DAESHI010000342 DAESHI010000000
VERSION     DAESHI010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.345"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..194
                     /locus_tag="KW011_004571"
     CDS             <1..194
                     /locus_tag="KW011_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HBH7662701.1"
                     /translation="VDLHQVLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNL
                     HYIDTGAVFGGELTLVQLQ"
     gene            complement(195..386)
                     /locus_tag="KW011_004572"
     CDS             complement(195..386)
                     /locus_tag="KW011_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HBH7662702.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(491..>650)
                     /locus_tag="KW011_004573"
     CDS             complement(491..>650)
                     /locus_tag="KW011_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HBH7662703.1"
                     /translation="LTIPCKSDPDLCMQLDAWDAETSIPALLNGEHSVLYRTRYDQQS
                     DAWIMRLA"
BASE COUNT          184 a          148 c          160 g          158 t
ORIGIN      
        1 acgttgattt gcatcaggtc ttgtggagcc gctcgcgatt aggtgaacgc caaaaagggc
       61 agggaattac aggtgctgat catttctggt ttggtcatac accgttgcga catcgcgtgg
      121 atattggcaa cctgcattat attgataccg gtgctgtctt tgggggcgaa ctgactcttg
      181 tgcaattgca ataattaaaa atcaccgtac tcctgtgcag gtcgccagaa accatctata
      241 aaatcctcaa tcggaaaaca accgccatgg cggatccgtt gatcgctcat agaataaaga
      301 cactgctgtt ccgtgttgta gacatccaca acaatatctt cacaaccgcc atccaggtag
      361 caaacaaaaa gtaccagcgc gaacatttca tccccgaagt gtggtgccgt accgttaagt
      421 ttaggagaga ttttacaacg ggggaataac caggacaaat aacccgccat cccgacgggt
      481 tctttttgga tcaggcaaga cgcataatcc aggcatcaga ttgttgatcg taacgggtac
      541 ggtaaaggac agagtgttcg ccatttagca gggcagggat actggtttca gcatcccagg
      601 catcgagttg catacataaa tcggggtcag atttacaagg aatggttaac
//
LOCUS       DAESHI010000343          650 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.346, whole genome shotgun sequence.
ACCESSION   DAESHI010000343 DAESHI010000000
VERSION     DAESHI010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.346"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(7..330)
                     /locus_tag="KW011_004574"
                     /pseudo
     CDS             complement(7..330)
                     /locus_tag="KW011_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            384..587
                     /locus_tag="KW011_004575"
                     /pseudo
     CDS             384..587
                     /locus_tag="KW011_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          158 a          175 c          154 g          163 t
ORIGIN      
        1 gcatcatttt ttccagaggc ggtcaaactc cgccttcgca aaataagcgg aagcctggcg
       61 aaggatatcg ttactgcggc gcagttcacg attttcacgc tccagctctt tcagacgctg
      121 acgttcagcg gtggtgagcc caccatcacc gcccccggta tcccgctcat gctggcgaac
      181 ccagacacgc agagtctccg gcgtacagcc aatctttggg gcaatggaac aaattgccgc
      241 ccactgtgag tcatattcat cctgactttc cagaaccata cgaatcgccc gctgacggac
      301 ttcgggggaa aaacgagtat ttttagtcat cctgtttacc tctttctcag ggagtttagt
      361 ctccaggatt tccggggcgg ttcagacatc tagaatttac tgtcagcacc ggcgttaaag
      421 tttacttctg cgatcctcag agtccttggc agcggggaac aaatgagaat acaaatgggc
      481 taattcggca gtactttcct aaaaagacat gtcttgccca atatactcaa catcgagtag
      541 gcagcctgac ggctgcggct tgtcatggtc tgcaattacc gttataaaaa caggcaatgt
      601 cattgtcttt caggtggtta tatggcccgt tcagctaaac cccgtaaacg
//
LOCUS       DAESHI010000344          641 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.347, whole genome shotgun sequence.
ACCESSION   DAESHI010000344 DAESHI010000000
VERSION     DAESHI010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.347"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..>641
                     /gene="fliD"
                     /locus_tag="KW011_004576"
     CDS             <1..>641
                     /gene="fliD"
                     /locus_tag="KW011_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBH7662704.1"
                     /translation="VSTEAGAAPGIYTISVTQLAQAQSLRTDSPTIIASTKDALGDES
                     SDTRTIKITQDGRKEPLEIKLNKDQTSLDEISKAINDADSGISASIVKVKDGNYQLVL
                     TASEGLANKMTISVEGDSKLNDLLAYDSKTNTGNMKELVNAQNAQLNVNGIDIERSSN
                     KITDAPQGVTLDLTKKVTDVRVTVTKSNDKATEAIKGWVDSYNSLIDTFNTLT"
BASE COUNT          217 a          146 c          139 g          139 t
ORIGIN      
        1 aagtttccac cgaagcaggt gctgcccccg gtatctatac catcagcgta acccaactgg
       61 cgcaggcgca atctttacga actgattccc cgacaatcat tgcatcaaca aaagatgcac
      121 tgggtgatga gtcatcagat acacgcacaa taaaaattac ccaagacggt cgtaaagagc
      181 cgctggaaat caagctcaat aaagatcaga cgtcattgga tgagatcagc aaagccatta
      241 acgatgccga tagtggaatt tccgctagta tcgttaaagt caaagatggt aactatcaat
      301 tggtattgac cgccagtgaa ggtctggcca ataaaatgac catatctgtt gagggtgaca
      361 gtaaattaaa tgatctgctg gcttatgaca gcaagaccaa caccgggaat atgaaggaac
      421 tggttaacgc gcaaaatgca cagctaaacg ttaacggtat cgatattgaa cgttcaagta
      481 acaaaattac cgatgctcct cagggagtga ctctcgattt gaccaaaaaa gtcactgacg
      541 tccgggtcac ggtgacaaaa agcaacgata aagcaaccga agcaattaaa ggctgggtgg
      601 attcatataa ctccctcatt gacaccttta acaccctgac a
//
LOCUS       DAESHI010000345          637 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.348, whole genome shotgun sequence.
ACCESSION   DAESHI010000345 DAESHI010000000
VERSION     DAESHI010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.348"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..>637
                     /locus_tag="KW011_004577"
     rRNA            <1..>637
                     /locus_tag="KW011_004577"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          152 a          143 c          209 g          133 t
ORIGIN      
        1 tcctcctaaa gagtaacgga ggagcacgaa ggttggctaa tcctggtcgg acatcaggag
       61 gttagtgcaa tggcataagc cagcttgact gcgagcgtga cggcgcgagc aggtgcgaaa
      121 gcaggtcata gtgatccggt ggttctgaat ggaagggcca tcgctcaacg gataaaaggt
      181 actccgggga taacaggctg ataccgccca agagttcata tcgacggcgg tgtttggcac
      241 ctcgatgtcg gctcatcaca tcctggggct gaagtaggtc ccaagggtat ggctgttcgc
      301 catttaaagt ggtacgcgag ctgggtttag aacgtcgtga gacagttcgg tccctatctg
      361 ccgtgggcgc tggagaactg aggggggctg ctcctagtac gagaggaccg gagtggacgc
      421 atcactggtg ttcgggttgt catgccaatg gcactgcccg gtagctaaat gcggaagaga
      481 taagtgctga aagcatctaa gcacgaaact tgccccgaga tgagttctcc ctgacccttt
      541 aagggtcctg aaggaacgtt gaagacgacg acgttgatag gccgggtgtg taagcgcagc
      601 gatgcgttga gctaaccggt actaatgaac cgtgagg
//
LOCUS       DAESHI010000346          629 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.349, whole genome shotgun sequence.
ACCESSION   DAESHI010000346 DAESHI010000000
VERSION     DAESHI010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.349"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..>629)
                     /locus_tag="KW011_004578"
     CDS             complement(<1..>629)
                     /locus_tag="KW011_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HBH7662705.1"
                     /translation="WFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIR
                     SVNTVGKSAFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIR
                     TSITDVSNEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIA
                     GIGAGIENTPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDV"
BASE COUNT          142 a          182 c          136 g          169 t
ORIGIN      
        1 acgtcgttca tgaatatctg atcgccctga ccaacaaaca tcggttttgt gttgccattc
       61 gcaggattaa ccatcgcaat cctgtccgcc gccagcagca cctgactctg catgccgtca
      121 ggggtgttct caataccggc accaataccc gcgatataaa ggcgtccgtc ctgcatctgc
      181 tgcagcttca cagcccacat gctgttcagg ttattatttg tatcaacctg aaccttctgt
      241 atctgctgaa ttgccgcact ctggtcttcc agtttcttat tgacggtctg cgtgatttca
      301 ttgctgacat ccgtaatgga cgtcctgatt tcagccaggt caggcgcaag ctgaccgtta
      361 tcaatctgcg tccacagctc ctgagccaga tgggttttcc ctatctcgcc tttgaaaaaa
      421 tccagataac cggatgcatc atcactcggc tgaccaacag cctccacgaa tgccgatttg
      481 ccaacggtgt tcacactgcg gatataaaag taataatcat ggcccggttt gatattgata
      541 ctggcggcta tccagtacag cgccgtgcca agatagcgtg ctgtggtttc aacctgcctg
      601 atatccgcaa tccgcttttc cgagaacca
//
LOCUS       DAESHI010000347          593 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.350, whole genome shotgun sequence.
ACCESSION   DAESHI010000347 DAESHI010000000
VERSION     DAESHI010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.350"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            289..>593
                     /gene="mcbR"
                     /locus_tag="KW011_004579"
     CDS             289..>593
                     /gene="mcbR"
                     /locus_tag="KW011_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HBH7662706.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAV"
BASE COUNT          172 a          121 c          149 g          151 t
ORIGIN      
        1 cggtttagag aatgcgccac agacgtttat cggcctgctg aagggtaaaa acttcggcaa
       61 agtggtgatc cgcgtcgcgg gtgatgatta aagtttcaac ggcggcgtaa gccgccataa
      121 aaatccaggc aaaaagctta ttttcagctt taattaacta accacgtcat tgttctgctg
      181 ctaataacaa caaaggtggg tggtttatga ttattcactt taatacacca ggtgaactcc
      241 ttctgccgtg caggcagggt tggacagaaa acacgcgaag gaattaaaat gcctggaacg
      301 ggaaaaatga aacatgtcag tttgactctg caggttgaga acgacctgaa acatcagctt
      361 agtattggcg cattaaaacc tggcgcacgc ctgattacta aaaatctggc ggagcaatta
      421 ggtatgagta ttacacctgt gcgtgaagca ttattacgtc tggtttcggt gaatgcgctt
      481 tctgtcgcac ctgcacaagc atttacagtt ccggaagtgg ggaaacgtca attagacgaa
      541 atcaatcgga tccgctacga actggaatta atggcagttg ctctggctgt tga
//
LOCUS       DAESHI010000348          581 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.351, whole genome shotgun sequence.
ACCESSION   DAESHI010000348 DAESHI010000000
VERSION     DAESHI010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.351"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            1..365
                     /locus_tag="KW011_004580"
                     /pseudo
     CDS             1..365
                     /locus_tag="KW011_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343122.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
     gene            275..502
                     /locus_tag="KW011_004581"
     CDS             275..502
                     /locus_tag="KW011_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HBH7662707.1"
                     /translation="MLPENAGSDQHNDSNNETGEDELNWKRQVLIAAVYGLCANPACI
                     AYAPAIPDIAMMIANKLENFGVTSDERLPDF"
     gene            480..>581
                     /locus_tag="KW011_004582"
     CDS             480..>581
                     /locus_tag="KW011_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HBH7662708.1"
                     /translation="MSAYPIFDRIEEMAWARHYQQIVREEKETELADD"
BASE COUNT          196 a          119 c          155 g          111 t
ORIGIN      
        1 gattagcgac ccgaatgaac cgaaatggat tcccggtcat catatctcct caaatgaagt
       61 caaaaaaacg gaaaatgaag tgcgacagac tgaagataaa cagcaccaga acagcgaacc
      121 ggaggaggga acaccctgca atcagactgg cgaggataac tgccctgact gtggcgcggt
      181 gatgggagac gcaacttatc aggaaacttt tgacgaaaaa aaaccagatg aagcacagga
      241 agaagagccg aagaaaacgg aaaaagctga tgatttgtta ccggagaacg ctggcagcga
      301 tcagcataat gatagcaata atgaaactgg cgaagatgaa ctcaactgga aaagacaggt
      361 gttgattgcg gcggtttacg gtttatgtgc gaatcctgcg tgcatagcct atgcgcctgc
      421 aatccctgac atcgcaatga tgattgccaa caagcttgaa aattttggag tgaccagtga
      481 tgagcgctta cccgattttt gatcgtattg aagaaatggc atgggctcgt cactaccagc
      541 agatcgttcg tgaagaaaaa gaaacggaac tggcggacga c
//
LOCUS       DAESHI010000349          573 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.352, whole genome shotgun sequence.
ACCESSION   DAESHI010000349 DAESHI010000000
VERSION     DAESHI010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.352"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..386
                     /locus_tag="KW011_004583"
     CDS             <1..386
                     /locus_tag="KW011_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBH7662709.1"
                     /translation="KGQVVELVRLSGRETLRQLEAKTGATRYLMSVLARELVASGDVY
                     NSGYGLFPSEQARKDWQNARKKLSRANLKKPSVVDPDLIWSLPDGEIRRYDRRQNIIC
                     SECRKSEAMQRVLAFYQGNFQKVLL"
     gene            514..>573
                     /locus_tag="KW011_004584"
     CDS             514..>573
                     /locus_tag="KW011_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662710.1"
                     /translation="MASVSISCPSCSATDGVVRN"
BASE COUNT          150 a          120 c          156 g          147 t
ORIGIN      
        1 ttaaagggca ggttgttgaa cttgtacgtc tgagcggtcg cgagacgttg cggcaactgg
       61 aagccaagac aggtgcgaca agatatctga tgagtgttct cgccagagag ctggttgcca
      121 gtggcgatgt atacaactct ggttacgggt tattcccgtc tgaacaggcg cgtaaggact
      181 ggcaaaacgc ccgcaaaaaa ctctcaaggg caaatctgaa gaaaccatct gtggttgatc
      241 ctgaccttat ctggtcatta ccagacggag aaatacgccg ctacgacagg cgtcaaaaca
      301 taatctgtag cgagtgccgg aagagcgaag ctatgcagcg tgtactggct ttctatcagg
      361 gtaattttca gaaggtgctg ttgtgagcca aattaacaat cggaacttcg tgaagagaaa
      421 gcataatcca aaatctgaat aattaagttc agcactgtgg tgatgctgcc aacttactga
      481 tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtttctat cagctgtccc
      541 tcctgttcag ctactgacgg ggtggtgcgt aac
//
LOCUS       DAESHI010000350          555 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.353, whole genome shotgun sequence.
ACCESSION   DAESHI010000350 DAESHI010000000
VERSION     DAESHI010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.353"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            78..555
                     /locus_tag="KW011_004585"
                     /pseudo
     CDS             78..555
                     /locus_tag="KW011_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT          136 a          121 c          123 g          175 t
ORIGIN      
        1 agctgataga aacagaagcc actggagcgc ctcaaaaaca ccatcataca ctaaatcagt
       61 aagttggcag catcaccgcc gttgtctaca catttattct ctcggtgctg atttaccggg
      121 aagtaaaatg gcgccattta cccaaactga ttctggagtc ggttgttacc acttcgattg
      181 tattactgtt gatcggtttt tccgtgggca tgtcctgggc aatgactaat gctgatattc
      241 cttatatgat cagcgacgca ttgatgggga tttctgataa cccactgatc atattgctgc
      301 tgattaatat cgtcttatta attgtcggca tctttatgga catgacaccg gctgtactaa
      361 tctttacacc gatcttcttg ccgattgcac aagagctggg aatggaccca gtacatttcg
      421 gtattatgat ggtggccaac ctttgtattg ggctgttaac accgccggtg ggtagtgcgc
      481 tatttgttgg ttgttctatc tcgggtgtga agattcaaca tttaatcaaa ccattgctgc
      541 cattttatgc tagca
//
LOCUS       DAESHI010000351          553 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.354, whole genome shotgun sequence.
ACCESSION   DAESHI010000351 DAESHI010000000
VERSION     DAESHI010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.354"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..>553
                     /gene="feaR"
                     /locus_tag="KW011_004586"
     CDS             <1..>553
                     /gene="feaR"
                     /locus_tag="KW011_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HBH7662711.1"
                     /translation="WQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDAS
                     RPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMNN
                     PALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEWI
                     AGETGMSVRSLYRMFADKGLVVAQ"
BASE COUNT          140 a          125 c          146 g          142 t
ORIGIN      
        1 tggcaggaag taaaaggcag cgacgatgcc tggttttaca ccgtttttca gcttagtggt
       61 caggcaataa tggagcagga tgagcgtcag gtgcagattg gcgctggcga tattacgtta
      121 ctcgatgcct cacgcccctg ttcgctttac tggcaggagt cttctaaaca gatttcatta
      181 cttttgccac gcactctgct ggaacaatat tttccccatc aaaaacctgt ctgcgcagaa
      241 agactggacg ctgacttacc catggtgcaa ctcagtcatc gcctgttaca ggagagcatg
      301 aataatccgg cactttctga aacagaaagt gaagctgcgc tacaggcgat ggtgtgtctg
      361 ctgcgcccgg tacttcatca gcgggaatct gttcaacctc gccgtgaacg tcagtttcaa
      421 aaagtggtta cgttgataga cgataatatt cgcgaagaga tattacgccc ggagtggata
      481 gccggagaga caggtatgtc agtacgtagt ttgtaccgaa tgtttgccga taaaggtttg
      541 gtagtcgcgc aat
//
LOCUS       DAESHI010000352          550 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.355, whole genome shotgun sequence.
ACCESSION   DAESHI010000352 DAESHI010000000
VERSION     DAESHI010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.355"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            1..550
                     /locus_tag="KW011_004587"
                     /pseudo
     CDS             1..550
                     /locus_tag="KW011_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
BASE COUNT          133 a          137 c          162 g          118 t
ORIGIN      
        1 ctcaacccgg tacgcaccag aaaatcattg atatggccat gaatggcgtt ggatgccggg
       61 caactgcacg cattatgggc gttggcctca acacgatttt acgtcactta aaaaactcag
      121 gccgcagtcg gtaacctcgc gcatacagcc gggcagtgac gtcatcgtct gcgcggaaat
      181 ggacgaacag tggggctatg tcggggctaa atcgcgccag cgctggctgt tttacgcgta
      241 tgacagtctc cggaagacgg ttgttgcgca cgtattcggt gaacgcacta tggcgacgct
      301 ggggcgtctt atgagcctgc tgtcaccctt tgacgtggtg atatggatga cggatggctg
      361 gccgctgtat gaatcccgcc tgaagggaaa gctgcacgta atcagcaagc gatatacgca
      421 gcgaattgag cggcataacc tgaatctgag gcagcacctg gcacggctgg gacggaagtc
      481 gctgtcgttc tcaaaatcgg tggagctgca tgacaaagtc atcgggcatt atctgaacat
      541 aaaacactat
//
LOCUS       DAESHI010000353          549 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.356, whole genome shotgun sequence.
ACCESSION   DAESHI010000353 DAESHI010000000
VERSION     DAESHI010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.356"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            1..549
                     /locus_tag="KW011_004588"
                     /pseudo
     CDS             1..549
                     /locus_tag="KW011_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT          195 a          121 c           83 g          150 t
ORIGIN      
        1 ccgacttatt aaaaatgcct gtttaaaatt atataattta gcacataccg caaccaactc
       61 ccaccttaaa ccaactggtc cggataattc agatgttctc ctgttcaaaa agctattctc
      121 cccttcgaaa ttaatgacaa ttatcggtga tgaaattcct cttatatctg aaaaacagtc
      181 gctttcaaag gtgcttttaa atgatgagaa taatgaactg agtgatggta caaacttctg
      241 ggataaaaat cgtcaattaa ccacagatga aatagcttgc tatcttcaga agatcgccgc
      301 taatgcaaaa aatactcaag tcaattatcc tactggtctc tacgtcccct actccaccag
      361 aactcacctg gaagacgctc tcaatgaaaa tattaagagc gatccatcat ggccgaatga
      421 agtccagtta ttccccataa atactggcgg acactggata ttagtttcgc tacagaaaat
      481 agtaaataaa aaaataataa actacaaata aaatgcgtca tattcaactc attgcgtgca
      541 ctaggccat
//
LOCUS       DAESHI010000354          546 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.357, whole genome shotgun sequence.
ACCESSION   DAESHI010000354 DAESHI010000000
VERSION     DAESHI010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.357"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            1..546
                     /locus_tag="KW011_004589"
                     /pseudo
     CDS             1..546
                     /locus_tag="KW011_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          159 a          128 c          144 g          115 t
ORIGIN      
        1 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
       61 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
      121 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
      181 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      241 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      301 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      361 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
      421 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
      481 cgctgtaagc agaaacgcaa gttcagagcg actacgaact cgaaccacaa tctgccagtt
      541 gcgcca
//
LOCUS       DAESHI010000355          532 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.358, whole genome shotgun sequence.
ACCESSION   DAESHI010000355 DAESHI010000000
VERSION     DAESHI010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.358"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            86..334
                     /locus_tag="KW011_004590"
     CDS             86..334
                     /locus_tag="KW011_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662712.1"
                     /translation="MFIYASGGNGGSAGGACANTSRLQGYVGGTLISVNASNNPAYGK
                     TAFISFAVPAGTSYQITSYPTENTSCGAGVFSVFGYQT"
     gene            complement(331..>532)
                     /locus_tag="KW011_004591"
     CDS             complement(331..>532)
                     /locus_tag="KW011_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662713.1"
                     /translation="TIGGKLKVTQLSTTGYLGQFDFCAIARMGNAEDAHYCQVVESPA
                     GSRKWYKYEHKTGCIASCVTLN"
BASE COUNT          135 a          118 c          126 g          153 t
ORIGIN      
        1 gacttctggt tcgctcaatg gttcttacac aaacttaggt tcacatagag gttcattctc
       61 agggcggaat tcagggggca gtacattgtt tatttatgca tctggaggta atggaggatc
      121 tgccggaggt gcatgtgcaa atacatcccg actgcaggga tatgttggtg ggacgctaat
      181 tagcgtgaat gccagcaata accctgcata tggaaaaaca gcctttatca gctttgctgt
      241 acctgcaggt acttcctatc agataacatc ctatccaaca gaaaatacat catgtggtgc
      301 cggggtattt tcagtatttg gatatcaaac ttaattgagc gttacacacg acgcaataca
      361 acctgtctta tgctcgtatt tgtaccattt gcgtgaacct gctgggctct caactacctg
      421 gcagtagtgg gcatcctctg cgttgcccat tctggcaatg gcacagaagt cgaattgccc
      481 cagataacct gtggtggaaa gctgagtaac tttgagtttt ccacctattg tc
//
LOCUS       DAESHI010000356          522 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.359, whole genome shotgun sequence.
ACCESSION   DAESHI010000356 DAESHI010000000
VERSION     DAESHI010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.359"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..>522)
                     /locus_tag="KW011_004592"
     CDS             complement(<1..>522)
                     /locus_tag="KW011_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH7662714.1"
                     /translation="FLGVTTWIFMAAGGAESVAVYVNDVKGGSKSFVKVIILAGIFIG
                     VLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAYFGLPEALMNRFVGLVSFTAMFGS
                     LLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPARAAWIQFLIVIPLMIIPMLGSNT
                     VQDLMNTIINMTA"
BASE COUNT          143 a          141 c          133 g          105 t
ORIGIN      
        1 cggcggtcat attaataata gtattcatca gatcctgcac ggtattggaa ccgagcatcg
       61 ggataatcat cagcgggatg acgatcagga actggatcca cgctgcgcgc gccggaacgc
      121 cgttttcgtt cagttcgacg gttttcttac caaagatgcc ttccgggatt tcggagaaga
      181 aaattttcac cggcgttgcg gtccacatca gcagggaacc gaacatcgcg gtaaaggaca
      241 ccagaccgac aaagcgattc atcaacgctt ccggtagacc aaaatacgcc gccatgccgt
      301 ggaatacctg caccgatccg ccggtaaatt tcaactcttt gctgctgacg aagacgttaa
      361 tcagcaccga ggagacggaa tacagtacgc cgataaaaat tccggcgagg atgatcactt
      421 taacgaacga tttcgaaccg cctttgacgt cgttaacgta cacagcgacg gactccgcgc
      481 cacctgcggc cataaagatc caggtggtaa cgccgaggaa cg
//
LOCUS       DAESHI010000357          522 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.360, whole genome shotgun sequence.
ACCESSION   DAESHI010000357 DAESHI010000000
VERSION     DAESHI010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.360"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            368..475
                     /locus_tag="KW011_004593"
     CDS             368..475
                     /locus_tag="KW011_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBH7662715.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT          133 a          124 c          158 g          107 t
ORIGIN      
        1 gtgggcgtca ggctgccgta atggcaattt gcgcccggac caggccgcag gagtgaaact
       61 ctgcggcctt tttcgtaaga aaaatgcaac ggttgcaagc ggataatatt ctgcggaatg
      121 cgcagcacag agtgaagatg agacggattc cggaacagaa aaacagtcgt agaatcagcg
      181 tcaagccgga aggattccgg tgaggcgcaa tgttgcgggg gcttaatccc tggtggcatt
      241 ggttgctgga gagagaaaac ccccgcacgt tgcaggtatg cacctgacaa caccacgggg
      301 gccattcttg catccaaaca atacaagaat agccgcgaaa cgttgtcatt acaatacagg
      361 cggctatatg acgctcgcag aactgggcat ggccttctgg catgatttag cggctccggt
      421 cattgctggt attcttgcca gtatgatcgt gaactggctg aacaagcgga agtaacgtgc
      481 catgcgggcg tcaggctgcc gtaatggcaa tttgcgcccg ga
//
LOCUS       DAESHI010000358          511 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.361, whole genome shotgun sequence.
ACCESSION   DAESHI010000358 DAESHI010000000
VERSION     DAESHI010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.361"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            1..511
                     /locus_tag="KW011_004594"
                     /pseudo
     CDS             1..511
                     /locus_tag="KW011_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          128 a          127 c          145 g          111 t
ORIGIN      
        1 attgaaatgg agaatgaaat attaaaaaag gctaccgcgc tcttgatgtc agactccctg
       61 aacagttctc gataatcggg aaactcagag cgcattatcc tgtggtcaca ctctgccatg
      121 tgttcggggt tcatcgcagc agctacagat actggaaaaa ccgtcctgaa aaaccagacg
      181 gcagacgggc tgtattacgc agtcaggtac ttgagctaca tggcatcagc cacggttcgg
      241 ccggagcaag aagcatcgcc acaatggcaa cccggagagg ctaccagatg ggacgctggc
      301 ttgctggcag gctcatgaaa gagctggggc tggtcagctg tcagcagccg actcaccggt
      361 ataaacgtgg tggtcatgaa catgttgcta tccctaacta ccttgaaagg cagttcgccg
      421 tgaccgagcc aaatcaggtg tggtgcggtg atgtgaccta tatctggacg ggtaagcgct
      481 gggcgtacct cgccgttgtt ctcgacctgt t
//
LOCUS       DAESHI010000359          507 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.362, whole genome shotgun sequence.
ACCESSION   DAESHI010000359 DAESHI010000000
VERSION     DAESHI010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.362"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..>507
                     /locus_tag="KW011_004595"
     CDS             <1..>507
                     /locus_tag="KW011_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBH7662716.1"
                     /translation="IPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYAEPLVESRYLYD
                     PLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSF
                     TPLIRVETATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRV
                     SEESRRWL"
BASE COUNT          110 a          147 c          171 g           79 t
ORIGIN      
        1 tcatcccgga aggggttatc cgcacggatg atgagcgcac ccaccggtac cattacgaca
       61 gtcagcaccg gctggtgcac tacacgcgga cacaatatgc agagccgctg gtcgaaagtc
      121 gctatcttta cgacccgctg ggccgcaggg tggcaaaacg ggtatggcgg cgtgaacggg
      181 acctgacggg ctggatgtcg ctgtcacgga aaccgcaagt gacctggtac ggctgggacg
      241 gcgaccggct gaccacaata cagaacgaca gaacccgcat ccagacgatt tatcagccgg
      301 ggagcttcac gccactcatc agggtcgaaa ctgccaccgg tgagctggcg aaaacgcagc
      361 gccgcagcct ggcggatgcc cttcagcagt ccggcggcga agacggtggc agtgtggtgt
      421 tcccgccggt gctggtgcag atgctcgacc ggctggaaag tgaaatcctg gctgaccggg
      481 tgagtgagga aagccgccgc tggctgg
//
LOCUS       DAESHI010000360          500 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.363, whole genome shotgun sequence.
ACCESSION   DAESHI010000360 DAESHI010000000
VERSION     DAESHI010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.363"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
BASE COUNT          154 a          100 c           93 g          153 t
ORIGIN      
        1 aggcttactt agtggaaaga gaggggatat gtgaccatag gggaacccct cctgttaaaa
       61 gactacttag aagtctgagg ttttagtaaa atctgtgaat atggcttctc agacgctctg
      121 taacgcattc taagcgattt taccccttta ttttaaggtg gttagtacat ccctataggt
      181 tacccctaaa aatggggtta aaactcgcta cagagctttc tatgagatta gcttctaatc
      241 gaatcaacta tgaggtttgg taggattgtg caaaatctgt acagtaactt ttcagaatct
      301 gaaaattttt ataaaaaatg actagccctc tttgaatctc atcttccccg aatcttcaaa
      361 aatatctgac agatgtctgt agctgctaag tacccgtcac accagctatg tgaccccctt
      421 ggcaaaaaag ccaaatatca gctacatcca tgtaactatt cagtaactta tttagcaagt
      481 ctgtaataag ttgctctaga
//
LOCUS       DAESHI010000361          489 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.364, whole genome shotgun sequence.
ACCESSION   DAESHI010000361 DAESHI010000000
VERSION     DAESHI010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.364"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="KW011_004596"
     CDS             complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="KW011_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HBH7662717.1"
                     /translation="TGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTML
                     AEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRT
                     EADRWALAEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQ
                     LTD"
BASE COUNT           99 a          153 c          117 g          120 t
ORIGIN      
        1 gtcagtcagc tgacggtaaa tctgctgttc agtctcacgc atcacctgtg caccggcttc
       61 cctctccgca tccgcatcac cgctcagacc tgatgctttc agccggtcag ccaccctctg
      121 agggtactca ttctccagca tctcatattt ctgctcttct gccagcgccc agcggtcagc
      181 ttccgtacgc ttcagtacag catgccatgg tccccagagg gagaaccagt ccgtaaattc
      241 attctcttca cggcttctga ccatggcttc ggcagtgcgg aggtcatttg ctgtcactcc
      301 cgacacgcca tagaaacgca tttccttcac ggcagtggag agctgaagtt tctctgcgag
      361 catggtctgg aaggccaggt agacttctat ctcatccaca aaatggagag ttctgacttt
      421 atcccgggca atgtcctcca gaatttcgag gcggaacatt tccctgccca gggagagcag
      481 agcgccggt
//
LOCUS       DAESHI010000362          485 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.365, whole genome shotgun sequence.
ACCESSION   DAESHI010000362 DAESHI010000000
VERSION     DAESHI010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.365"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..>485)
                     /locus_tag="KW011_004597"
     CDS             complement(<1..>485)
                     /locus_tag="KW011_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBH7662718.1"
                     /translation="IATVASMLLMFPWDIAAITVSQRLANGLDNFALLAIPFFIFAGT
                     LMNSGGIAIRLINLAQVMVGRVPGSLGHVNVLANMMFGSISGSAVAAAAAVGGTLNPI
                     QTKQGYDPAFSTAVNVSSCITGLLIPPSNVLIVFSLTAGGVSVASLFMAGYLPGILMG
                     L"
BASE COUNT          158 a          121 c          114 g           92 t
ORIGIN      
        1 caagacccat taagatccct ggcaaatatc ccgccataaa tagcgatgct acagaaacgc
       61 cgccagctgt cagggaaaac acaattaaca cgtttgaggg cggtatcagt aatccagtaa
      121 tacaagatga gacgtttaca gcggtagaaa atgccggatc ataaccttgt ttggtctgaa
      181 tgggattcag tgtgccacca acggcagcag ctgccgcaac ggctgaacca gaaatggaac
      241 caaacatcat attcgccagg acgttcacat gaccaagtga accaggaacc cgaccaacca
      301 ttacctgggc gaggttaatc aaacgtatcg caataccgcc gctgttcatc agcgttccgg
      361 caaagatgaa aaaggggatt gccaataggg caaagttatc cagtccatta gccagtcgct
      421 gcgaaacagt gattgccgcg atatcccagg gaaacatcaa aagcattgaa gctacagtgg
      481 caata
//
LOCUS       DAESHI010000363          476 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.366, whole genome shotgun sequence.
ACCESSION   DAESHI010000363 DAESHI010000000
VERSION     DAESHI010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.366"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..>476
                     /locus_tag="KW011_004598"
     rRNA            <1..>476
                     /locus_tag="KW011_004598"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAESHI010000364          472 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.367, whole genome shotgun sequence.
ACCESSION   DAESHI010000364 DAESHI010000000
VERSION     DAESHI010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.367"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            89..451
                     /locus_tag="KW011_004599"
     CDS             89..451
                     /locus_tag="KW011_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBH7662719.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKD"
BASE COUNT          114 a          112 c          138 g          108 t
ORIGIN      
        1 cccggtggtg atgtctatct cacaccgatg aacatgacca cgaaaccctc cgatggcagt
       61 aaggccggta agcagaagga taacgccaat gcagacgaaa caacgtcttg atgtaccgct
      121 gagtctgaaa tctgtcagtg actccggtga gtttgaaggg tatggctccg tctttggtgt
      181 aaaggacagc cacgatgatg tggtgatgtc cggggcattt gctgcttccc tgcgggcgtg
      241 gagtgacaga aaagcgttac ctgcgctgct ctggcagcac cgcatggatg aacccatcgg
      301 tgtttacacc gaaatgaagg aagacgatgt cgggctttac gtcaggggac ggttgcttat
      361 tgatgatgat cccctcgcaa aacgtgcaca tgcacacatg aaggccggtt cgttaaccgg
      421 cctttctatt gggtacgtcc tgaaagactg aggtagcctg agtttaacgg ac
//
LOCUS       DAESHI010000365          463 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.368, whole genome shotgun sequence.
ACCESSION   DAESHI010000365 DAESHI010000000
VERSION     DAESHI010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.368"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..>463
                     /locus_tag="KW011_004600"
     CDS             <1..>463
                     /locus_tag="KW011_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBH7662720.1"
                     /translation="VKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTG
                     GFNSETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTA
                     VRSPESNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSA"
BASE COUNT          119 a          108 c          149 g           87 t
ORIGIN      
        1 gtgaaagaaa gtaatcagcg atggtgctct gacgggttcg agttccgctg tgataacgga
       61 gaaaaactgc gagtcacgtt cgcgctggac tgctgtgacc gtgaggcact gcactgggcg
      121 gtcactacgg gcggcttcaa cagtgaaaca gtacaggacg tcatgctggg agcggtggaa
      181 cgccgcttcg gcaacgagct tccggcgtct ccagtagagt ggctgacgga taatggttca
      241 tgctaccggg ctaatgaaac acggcagttt gcccggatgt tggggcttga accgaagagc
      301 acggcggtgc ggagtccgga gagtaacggc atagcagaga gcttcgtgaa aacgataaag
      361 cgtgactaca tcagtgtcat gcccaaacca gacgggttaa cggcagcaaa gaaccttgca
      421 gaggcgttcg agcattataa cgaatggcat ccgcatagtg cac
//
LOCUS       DAESHI010000366          457 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.369, whole genome shotgun sequence.
ACCESSION   DAESHI010000366 DAESHI010000000
VERSION     DAESHI010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.369"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..118
                     /locus_tag="KW011_004601"
     CDS             <1..118
                     /locus_tag="KW011_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HBH7662721.1"
                     /translation="KLTKSECEKMLSGEKEAGASREQKVKLINFNYEKLQSS"
     gene            225..>457
                     /locus_tag="KW011_004602"
     CDS             225..>457
                     /locus_tag="KW011_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBH7662722.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPL"
BASE COUNT          162 a           93 c           98 g          104 t
ORIGIN      
        1 aaaactaacg aaatcagaat gcgaaaaaat gctctcaggg gaaaaagaag caggcgcttc
       61 cagagagcag aaagtaaaac tgataaattt taattacgag aaacttcagt cctcgtgaat
      121 ttcattatat ccagattaga acttcatagc tgattattaa aaatcaacca cacccgccag
      181 tattctgtat atttactggc ggtcatatcg tgagaggtat ggcaatgaat cttgtgacac
      241 tcaaaacgtg gggaaaactc agatatccgg ataacccacc atcaatatca acgctgagac
      301 gatgggcaag gaatggaaac atttatcctg cacctgaact acacgggagg agttacaggg
      361 tggttccgga ggctttctat atcaacccaa ataaggttga taccgatata acacaccatc
      421 agcctaatgg gcgacaaggg agagacagtc cgttaat
//
LOCUS       DAESHI010000367          453 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.370, whole genome shotgun sequence.
ACCESSION   DAESHI010000367 DAESHI010000000
VERSION     DAESHI010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.370"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
BASE COUNT          129 a          106 c           89 g          129 t
ORIGIN      
        1 gcgctcctaa aattcaattc acaacgcaga cctacaacat agctaaaaac actcgtaatt
       61 taaggctcgg ggtccatgca tattgctcat ggacttacct gaatggctca ccctttggtg
      121 gttttcaaca ggtatattcc gaccaaaaca acgtttggta tgtgagtaat tatgcttggg
      181 gaaattatga gtctggtggg accatatcag tcacatgcct caatcttcct ggtgctggag
      241 cttaattaaa ggggcaacag taggcatcga catgttcctc atcaacacca tcaccaatac
      301 cgtacatgcg tgttccagtc ataatgtatc cagtcggaca ttgaatcggc ttgtaataga
      361 tcgaaccacc actcttaccg ccgataaagt gattcattgc tacggatgat ttgtaccaat
      421 tacatgctga gtaattaatt ttattacccc ctg
//
LOCUS       DAESHI010000368          448 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.371, whole genome shotgun sequence.
ACCESSION   DAESHI010000368 DAESHI010000000
VERSION     DAESHI010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.371"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..238)
                     /locus_tag="KW011_004603"
     CDS             complement(<1..238)
                     /locus_tag="KW011_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HBH7662723.1"
                     /translation="METCFKILQLKFDKKLTNRCIGLTLHISASTVFEVLARFKASSL
                     SWPLPADISHDTLEKLIFPPKDTSASELVMPDMLY"
BASE COUNT          127 a           88 c          119 g          114 t
ORIGIN      
        1 agtacagcat gtcaggcata acaagctctg acgcagaggt gtctttgggc gggaaaatga
       61 gtttctccag cgtgtcatgg gatatatccg caggtaatgg ccatgacaga gaagaggctt
      121 tgaagcgggc aagaacctca aaaacggttg aggcgctgat gtgtaacgtc agtccgatac
      181 aacggttagt cagcttctta tcgaacttga gctgaaggat cttaaaacaa gtttccatag
      241 tgctcctgga ttttctcttt ttggacattg acgtctccac tggtttaaac acggcaatgg
      301 agactgtggt gaaaagagtt aattcccgga gtgactggcg aattccaatc aatgatcgga
      361 agtatgccaa atcgtgatcg attgtgatgc caaactgtga tcggagatgg atgccaaatc
      421 atgatcgatt cgaatgccaa actgcgat
//
LOCUS       DAESHI010000369          435 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.372, whole genome shotgun sequence.
ACCESSION   DAESHI010000369 DAESHI010000000
VERSION     DAESHI010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.372"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..>435
                     /locus_tag="KW011_004604"
     CDS             <1..>435
                     /locus_tag="KW011_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH7662724.1"
                     /translation="HIAANIQFPPNFDESKQYPAIISVHPFGSCKEQTSGNIYGKALA
                     EKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLPYVNAERIGVLGVC
                     GGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIG"
BASE COUNT          108 a           84 c          123 g          120 t
ORIGIN      
        1 ggcatattgc tgccaatatt cagtttccac caaattttga tgagtcaaaa cagtatccgg
       61 cgattatcag cgtccatcct tttggcagtt gtaaggagca gacgtctggc aatatctatg
      121 gcaaggcatt ggctgaaaaa ggctatctcg ttctcgctta tgacgccagt ttccagggag
      181 aatctggtgg ggagcctcgc tggatagaag atccgacaca gcgcgtagag gatattagcc
      241 gtgttatcga ttatgcagta actctgccat atgttaatgc tgaacgtatt ggcgtgctgg
      301 gagtgtgtgg gggtggtggc tatgccatta atgccgcgct gacagaaaaa cgaattaaag
      361 cagtagtgag tattacgggt gttaatatcg ggcgtctttt tcgggaaggt ttcagcaatt
      421 acgatccgat tggcg
//
LOCUS       DAESHI010000370          422 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.373, whole genome shotgun sequence.
ACCESSION   DAESHI010000370 DAESHI010000000
VERSION     DAESHI010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.373"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            41..116
                     /locus_tag="KW011_004605"
     tRNA            41..116
                     /locus_tag="KW011_004605"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74..76,aa:Ile,seq:cat)
BASE COUNT          119 a           79 c           98 g          126 t
ORIGIN      
        1 tttattgcgt ggtttataag tattgttatt gcacactcaa ggccctttag ctcagtggtt
       61 agagcaggcg actcataatc gcttggtcgc tggttcaagt ccagcaaggg ccaccaaatc
      121 attatttacg tatcaggaag agttaagggt acaaggggta ctcgatggcg gtctatattt
      181 catgactaac gccatttcaa ggaagaagtg cggtgggtgg tttcgaatga gggctggtgt
      241 tttggtacgt ggcacgccac tgcaatgtat ttatccatca gtactcattc aaaagtacct
      301 ggaaaaaatg cctgctatgc caagcgggca aattgcttac atgtggaatt aagtaattaa
      361 cgataaagcc agagtattat catttattat accccttcat ttaatcaggt taattactca
      421 aa
//
LOCUS       DAESHI010000371          420 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.374, whole genome shotgun sequence.
ACCESSION   DAESHI010000371 DAESHI010000000
VERSION     DAESHI010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.374"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(76..345)
                     /locus_tag="KW011_004606"
     CDS             complement(76..345)
                     /locus_tag="KW011_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBH7662725.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
BASE COUNT          109 a          108 c           81 g          122 t
ORIGIN      
        1 gctctgacaa cgatgttaac ccgcgttaaa tatcccacgt tggccagcat ctgtctcacg
       61 gaaagtcata ctgccttaca ttgtgtctgg ttcttccggc cactcaatat caggtgcagt
      121 tgatgtatca acacggttca gcaacacccg atacttcttc caggcttcca gcaacgagtt
      181 ttcttcctcc gttgcgatct ccagatctac agcatcctga agtggcgcaa tatgctcact
      241 gaattcctgg atgtagaact gtgtggtgac ggtcttccag ccattcggct cctgctgtat
      301 cgaagcatac caggctattt caatatcgct atgctgcggc agcatttaac cccttgtaat
      361 tcatcgccat aattgattta attcacaaat aaaactataa tatggtgaaa ttaatgaaaa
//
LOCUS       DAESHI010000372          408 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.375, whole genome shotgun sequence.
ACCESSION   DAESHI010000372 DAESHI010000000
VERSION     DAESHI010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.375"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            54..130
                     /locus_tag="KW011_004607"
     tRNA            54..130
                     /locus_tag="KW011_004607"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="KW011_004608"
     tRNA            173..248
                     /locus_tag="KW011_004608"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
BASE COUNT          115 a           86 c           97 g          110 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcg
//
LOCUS       DAESHI010000373          403 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.376, whole genome shotgun sequence.
ACCESSION   DAESHI010000373 DAESHI010000000
VERSION     DAESHI010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.376"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(83..>403)
                     /locus_tag="KW011_004609"
     CDS             complement(83..>403)
                     /locus_tag="KW011_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HBH7662726.1"
                     /translation="AAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAGSVI
                     VTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLKQPA
                     QEAS"
BASE COUNT          139 a           89 c           79 g           96 t
ORIGIN      
        1 ccgattgaga acgaaatggg cacaccaata aagaccagca caaaaaaaca gccaaacagt
       61 gtaatcgcga tataactatc cattaactgg cctcctgcgc aggttgtttc agttgttgaa
      121 tgctttgatt aataaaacac aaggcataaa acatcatcac caatccactt aatggcagaa
      181 tgatatatac gtatcccatc ggtatttgca tcgcggcaga tacctgtgat gttgccagcg
      241 ttttatcaat taatttcagg ccaccagtca caatgaccga accagcaaaa cctaaaataa
      301 gaacgttaat gactatacta agaagtaatt gcttgcgctt attcagggcg agagcaaata
      361 aatcgataga gagatgtctc tgcgcgccga cggtatatgc agc
//
LOCUS       DAESHI010000374          398 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.377, whole genome shotgun sequence.
ACCESSION   DAESHI010000374 DAESHI010000000
VERSION     DAESHI010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.377"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..>398
                     /locus_tag="KW011_004610"
     CDS             <1..>398
                     /locus_tag="KW011_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HBH7662727.1"
                     /translation="QSKSLGESLGGLMSDMSHGVDSLTDISYRVRAFFDEVTLAYRSL
                     NDTQKAVADGLANGLLSALKGLAGIVAVRSGIGAVGGIWNLIRAISTLGNVANTAAGR
                     INSRSTGSVEGGKSRLSVADAITRVMIVGM"
BASE COUNT          105 a           64 c          110 g          119 t
ORIGIN      
        1 tcaatctaaa agtcttggtg aatctcttgg tggcttgatg agcgatatgt cgcatggggt
       61 cgattcacta accgatatta gttacagggt acgagcgttc tttgatgagg tgacattagc
      121 ttatcgttca ctaaatgaca ctcaaaaagc tgtagctgat ggtcttgcaa atggcctgtt
      181 aagtgcatta aaggggcttg ctggtatagt cgctgtacgg tctgggattg gtgctgtagg
      241 tggtatctgg aacttaatta gggccatttc cacgcttgga aatgtggcaa atacagcagc
      301 aggaagaatc aattcacgtt ctactggtag tgttgaaggt ggaaaaagta ggctatctgt
      361 agctgatgca attaccagag taatgattgt cggtatga
//
LOCUS       DAESHI010000375          367 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.378, whole genome shotgun sequence.
ACCESSION   DAESHI010000375 DAESHI010000000
VERSION     DAESHI010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.378"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..>367
                     /locus_tag="KW011_004611"
     CDS             <1..>367
                     /locus_tag="KW011_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBH7662728.1"
                     /translation="SRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQ
                     IRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYSALRPH
                     EYNGGLPPNESENRYWKNSN"
BASE COUNT          106 a           85 c          107 g           69 t
ORIGIN      
        1 agcaggctca ccatgaaagc gctggaaatg gcatgggaaa cccgtggtaa gcccggcggg
       61 gtgatgttcc acagcgatca gggcagtcat tatacgagca ggcagttccg gcagttattg
      121 tggcgatacc agatcagaca gagtatgagc cggcgcggaa actgctggga taacagccca
      181 atggaacgct tcttcaggag tctgaagaac gaatggatgc cggtggtggg ttacgtaagc
      241 ttcagcgagg cagctcacgc cataacggac tatatcgttg gatattacag cgcactaaga
      301 ccgcacgaat ataacggtgg gttaccccca aacgaatcgg aaaatcgata ctggaaaaac
      361 tctaact
//
LOCUS       DAESHI010000376          357 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.denovo.379, whole genome shotgun sequence.
ACCESSION   DAESHI010000376 DAESHI010000000
VERSION     DAESHI010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.denovo.379"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            <1..>357
                     /locus_tag="KW011_004612"
     rRNA            <1..>357
                     /locus_tag="KW011_004612"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           95 a           82 c          105 g           75 t
ORIGIN      
        1 taaaaacaca gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt
       61 gccggaaggt taattgatgg ggtcagcgca agcgaagctc ttgatcgaag ccccggtaaa
      121 cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac
      181 ctgcacgaat ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac
      241 tcgctgtgaa gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat
      301 agcttgacac tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtg
//
LOCUS       DAESHI010000377        20715 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.guided.071, whole genome shotgun sequence.
ACCESSION   DAESHI010000377 DAESHI010000000
VERSION     DAESHI010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.guided.071"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            complement(<1..683)
                     /gene="epmA"
                     /locus_tag="KW011_004613"
     CDS             complement(<1..683)
                     /gene="epmA"
                     /locus_tag="KW011_004613"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HBH7662729.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQIST"
     gene            1008..2816
                     /gene="frdA"
                     /locus_tag="KW011_004614"
     CDS             1008..2816
                     /gene="frdA"
                     /locus_tag="KW011_004614"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBH7662730.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            2809..3543
                     /gene="frdB"
                     /locus_tag="KW011_004615"
     CDS             2809..3543
                     /gene="frdB"
                     /locus_tag="KW011_004615"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HBH7662731.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            3554..3949
                     /gene="frdC"
                     /locus_tag="KW011_004616"
     CDS             3554..3949
                     /gene="frdC"
                     /locus_tag="KW011_004616"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBH7662732.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            3960..4319
                     /gene="frdD"
                     /locus_tag="KW011_004617"
     CDS             3960..4319
                     /gene="frdD"
                     /locus_tag="KW011_004617"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBH7662733.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            4382..5515
                     /locus_tag="KW011_004618"
     CDS             4382..5515
                     /locus_tag="KW011_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBH7662734.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            5604..6137
                     /gene="blc"
                     /locus_tag="KW011_004619"
     CDS             5604..6137
                     /gene="blc"
                     /locus_tag="KW011_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBH7662735.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6134..6451)
                     /gene="sugE"
                     /locus_tag="KW011_004620"
     CDS             complement(6134..6451)
                     /gene="sugE"
                     /locus_tag="KW011_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBH7662736.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(6626..6772)
                     /gene="ecnB"
                     /locus_tag="KW011_004621"
     CDS             complement(6626..6772)
                     /gene="ecnB"
                     /locus_tag="KW011_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBH7662737.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(6883..7008)
                     /gene="ecnA"
                     /locus_tag="KW011_004622"
     CDS             complement(6883..7008)
                     /gene="ecnA"
                     /locus_tag="KW011_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBH7662738.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7060..7626)
                     /gene="efp"
                     /locus_tag="KW011_004623"
     CDS             complement(7060..7626)
                     /gene="efp"
                     /locus_tag="KW011_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBH7662739.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            7668..8696
                     /gene="epmB"
                     /locus_tag="KW011_004624"
     CDS             7668..8696
                     /gene="epmB"
                     /locus_tag="KW011_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBH7662740.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9086..9955
                     /locus_tag="KW011_004625"
     CDS             9086..9955
                     /locus_tag="KW011_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HBH7662741.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLNAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGTVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(10158..10511)
                     /locus_tag="KW011_004626"
     CDS             complement(10158..10511)
                     /locus_tag="KW011_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HBH7662742.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(10649..12295)
                     /gene="groL"
                     /locus_tag="KW011_004627"
     CDS             complement(10649..12295)
                     /gene="groL"
                     /locus_tag="KW011_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBH7662743.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(12339..12632)
                     /locus_tag="KW011_004628"
     CDS             complement(12339..12632)
                     /locus_tag="KW011_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBH7662744.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            12908..14164
                     /gene="yjeH"
                     /locus_tag="KW011_004629"
     CDS             12908..14164
                     /gene="yjeH"
                     /locus_tag="KW011_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HBH7662745.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(14186..14656)
                     /gene="fxsA"
                     /locus_tag="KW011_004630"
     CDS             complement(14186..14656)
                     /gene="fxsA"
                     /locus_tag="KW011_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HBH7662746.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            14993..16429
                     /gene="aspA"
                     /locus_tag="KW011_004631"
     CDS             14993..16429
                     /gene="aspA"
                     /locus_tag="KW011_004631"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HBH7662747.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            16547..17848
                     /gene="dcuA"
                     /locus_tag="KW011_004632"
     CDS             16547..17848
                     /gene="dcuA"
                     /locus_tag="KW011_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HBH7662748.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            17964..18302
                     /gene="cutA"
                     /locus_tag="KW011_004633"
     CDS             17964..18302
                     /gene="cutA"
                     /locus_tag="KW011_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HBH7662749.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            18278..19975
                     /gene="dsbD"
                     /locus_tag="KW011_004634"
     CDS             18278..19975
                     /gene="dsbD"
                     /locus_tag="KW011_004634"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HBH7662750.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            20012..20587
                     /locus_tag="KW011_004635"
     CDS             20012..20587
                     /locus_tag="KW011_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH7662751.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         5058 a         5345 c         5223 g         5089 t
ORIGIN      
        1 tcggtactga tttgcgccag tgatgcctgg ctggctggaa agtggtacac aaaggtcggt
       61 ttttctttgc caatatttgg ctctacgcca aaggtaaaca gcaattgtag caacgtgtcg
      121 cggtcttctt cggtatcagc aacgttactc aaatccagtt tcgctgcgac ttcccgcagt
      181 tgcgttttgt cggcagagag cgggtcaatt tccagataac gcaagaaagc ttgttgataa
      241 gaaaggcttt ctgctgccgg gcagtccagc acctgttgta agagatcgtc cacctcgttc
      301 atcaaccggt acatatcata gtgcggtcga taccactcaa gcatagtgaa ctcagggttg
      361 tgataacgcc ccatctcttc attacggaag ctgcggcaca gctggaatac cggcccacaa
      421 ccggcaacca gcaggcgttt catatggtat tccgggctgg tcattaacca gagattcatc
      481 ccctgcgaat gcccggggcc aacgaaacgt gtctcaaacg ggaccaaatg aatatcggtt
      541 accgtcgcct ggctcataca aggcgtctcc acctccagca ctccacgatc ggcaaagaaa
      601 cgacggatct ccgccataat cgccgcacgt tttaataagt taggaatgga tgcgctcggc
      661 tgccaggatg ccgtttcgct catagttaaa tctccagttt ttgacaaggg cacgaagtct
      721 actcgcaacg cgacggcgag acaaatttta cgcaggaatc aaacagcggt gggcagtgac
      781 taaaaaaagc acgatctgat ggtttagtaa ttaaattaat catcttcagt gataatttag
      841 ccctcttgcg cactaaaaaa atcgatctcg tcaaatttca gacttatcca tcagactata
      901 ctgttgtacc tataaaggag cagtggaata gcgttcgcag accgtaactt tcaggcactt
      961 accctgaagt acggggctgt gggataaaaa caatctggag gaatgtcgtg caaacctttc
     1021 aagccgatct tgccattgta ggcgccggtg gcgcgggatt acgtgctgca attgctgccg
     1081 cgcaggcaaa tccaaatgca aaaatcgcac taatctcaaa agtatacccg atgcgtagcc
     1141 ataccgttgc tgcagaaggg ggctccgccg ctgtcgcgca ggatcatgac agcttcgaat
     1201 atcactttca cgatacagta gcgggtggcg actggttgtg tgagcaggat gtcgtggatt
     1261 atttcgtcca ccactgccca accgaaatga cccaactgga actgtggggg tgcccatgga
     1321 gccgtcgccc ggatggtagc gtcaacgtac gtcgcttcgg cggcatgaaa atcgagcgca
     1381 cctggttcgc cgccgataag accggcttcc atatgctgca cacgctgttc cagacctctc
     1441 tgcaattccc gcagatccag cgttttgacg aacatttcgt gctggatatt ctggttgatg
     1501 atggtcatgt tcgcggcctg gtagcaatga acatgatgga aggcacgctg gtgcagatcc
     1561 gtgctaacgc ggtcgttatg gctactggcg gtgcgggtcg cgtttatcgt tacaacacca
     1621 acggcggcat cgttaccggt gacggtatgg gtatggcgct aagccacggc gttccgctgc
     1681 gtgacatgga attcgttcag tatcacccaa ccggtctgcc aggttccggt atcctgatga
     1741 ccgaaggctg ccgtggtgaa ggcggtattc tggtcaacaa aaatggctac cgttatctgc
     1801 aagattacgg catgggcccg gaaactccgc tgggcgagcc gaaaaacaaa tatatggaac
     1861 tgggtccacg cgacaaagtt tctcaggcct tctggcacga atggcgtaaa ggcaacacca
     1921 tctccacgcc gcgtggcgat gtggtttatc tcgacttgcg tcacctcggc gagaaaaaac
     1981 tgcatgaacg tctgccgttc atctgcgaac tggcgaaagc gtacgttggc gtcgatccgg
     2041 ttaaagaacc gattccggta cgtccgaccg cacactacac catgggcggt atcgaaaccg
     2101 atcagaactg tgaaacccgc attaaaggtc tgttcgccgt gggtgaatgt tcctctgttg
     2161 gtctgcacgg tgcaaaccgt ctgggctcca actccctggc ggaactggtg gtcttcggcc
     2221 gtctggccgg tgaacaagcg acagagcgtg cagcaactgc cggtaatggc aacgaagcgg
     2281 caattgaagc gcaggcagct ggcgttgaac aacgtctgaa agatctggtt aaccaggatg
     2341 gcggcgaaaa ctgggcgaag atccgcgacg aaatgggcct ggcaatggaa gaaggttgcg
     2401 gtatctaccg tacgccggaa ctgatgcaga aaaccatcga caagctggca gagctgcagg
     2461 aacgcttcaa gcgcgtgcgc atcaccgaca cctccagcgt gttcaacacc gacctgctct
     2521 acaccattga actgggccac ggtctgaacg ttgctgaatg tatggcgcac tccgcaatgg
     2581 cacgtaaaga gtcccgcggc gcacaccagc gtctggacga aggttgcacc gagcgtgacg
     2641 acgtcaactt cctcaaacac accctcgcct tccgcgatgc tgatggcacg actcgcctag
     2701 agtacagcga cgtgaagatt actacgctgc cgccagctaa acgcgtttac ggtggcgaag
     2761 cggatgcagc cgataaggcg gaagcagcca ataagaagga gaaggcgaat ggctgagatg
     2821 aaaaacctga aaattgaggt ggtgcgctat aacccggaag tcgataccgc accgcatagc
     2881 gcattctatg aagtgcctta tgacgcaact acctcattac tggatgcgct gggctacatc
     2941 aaagacaacc tggcaccgga cctgagttac cgctggtcct gccgtatggc gatttgtggt
     3001 tcctgcggca tgatggttaa caacgtgcca aaactggcat gtaaaacctt cctgcgtgat
     3061 tacaccgacg gtatgaaggt tgaagcgtta gctaacttcc cgattgaacg cgatctggtg
     3121 gtcgatatga cccacttcat cgaaagtctg gaagcgatca aaccgtacat catcggcaac
     3181 tcccgcaccg cggatcaggg tactaacatc cagaccccgg cgcagatggc aaagtatcac
     3241 cagttctccg gttgcatcaa ctgtggtctg tgctacgccg cgtgcccgca gtttggcctg
     3301 aacccagagt tcatcggtcc ggctgccatt acgctggcgc atcgttataa cgaagatagc
     3361 cgcgaccacg gtaagaagga gcgtatggcg cagttgaaca gccagaacgg cgtatggagc
     3421 tgtactttcg tgggctattg ctccgaagtc tgcccgaaac acgtcgatcc ggctgcggcc
     3481 attcagcagg gcaaagtaga aagttcgaaa gactttctta tcgcgaccct gaaaccacgc
     3541 taaggagtgc aacatgacga ctaaacgtaa accgtatgta cggccaatga cgtccacctg
     3601 gtggaaaaaa ttgccgtttt atcgctttta catgctgcgc gaaggcacgg cggttccggc
     3661 tgtgtggttc agcattgaac tgattttcgg gctgtttgcc ctgaaaaatg gcccggaagc
     3721 ctgggcggga ttcgtcgact ttttacaaaa cccggttatc gtgatcatta acctgatcac
     3781 tctggcggca gctctgctgc acaccaaaac ctggtttgaa ctggcaccga aagcggccaa
     3841 tatcattgta aaagacgaaa aaatgggacc agagccaatt atcaaaagtc tctgggcggt
     3901 aactgtggtt gccaccatcg taatcctgtt tgttgccctg tactggtaag gagcctgaga
     3961 tgattaatcc aaatccaaag cgttctgacg aaccggtatt ctggggcctc ttcggggccg
     4021 gtggtatgtg gagcgcaatt attgcgccgg tgatgatcct gctggtgggt attctgctgc
     4081 cactggggct gtttccgggt gatgcgctga gctacgagcg cgttctggcg ttcgcgcaga
     4141 gcttcattgg tcgcgtattc ctgttcctga tgatcgttct gccgctgtgg tgtggtttac
     4201 accgtatgca ccacgcgatg cacgatctga aaattcacgt gcctgcgggc aaatgggttt
     4261 tctacggtct ggctgctatc ctgacagttg tcacgctgat tggtatcgtt acaatctaac
     4321 gtatcgccaa tgtaaatccg gcccgcctat ggcgggccgt tttgtatgga aaccagacct
     4381 tatgttcaaa acgacgctct gcgccttatt aattaccgcc tcttgctcca catttgctgc
     4441 ccctcaacaa atcaacgata ttgtgcatcg cacaattacc ccgcttatag agcaacaaaa
     4501 gatccccggt atggcggtgg cggtaattta tcagggtaaa ccttattact ttacctgggg
     4561 ctatgcggac atcgccaaaa agcagcccgt cacacagcaa acgttgtttg agttaggttc
     4621 ggtcagcaaa acatttacgg gcgtgcttgg tggcgacgct attgctcgag gggaaatcaa
     4681 gttaagcgat cccgcaacaa aatactggcc tgaacttact gctaaacagt ggaatgggat
     4741 cacactatta catcttgcga cctacaccgc tggcggcctg ccattgcagg tgccggatga
     4801 agtgaaatcc tcaagcgact tgctgcgctt ctatcaaaac tggcagcctg catgggctcc
     4861 aggaacacaa cgtctgtatg ccaactccag tatcggtttg ttcggcgcac tggctgtgaa
     4921 gccgtctggt ttgagttttg agcaggcgat gcaaactcgt gtcttccagc cactcaaact
     4981 caaccatacg tggattaatg tacctccccc agaagaaaag aattacgcct ggggatatcg
     5041 cgaaggtaag gcagtgcatg tttcgccagg ggcgttagat gctgaagctt atggtgtgaa
     5101 gtcgaccatt gaagatatgg cccgctgggt gcaaagcaat ttaacccccc ttgatatcaa
     5161 tgagaaaacg cttcaacaag ggatacaact ggcacaatct cgctactggc aaaccggcga
     5221 tatgtatcag ggcctgggct gggaaatgct ggactggccg gtcaatcctg acagcatcat
     5281 taacggcagt gacaataaaa ttgcactggc agcacgcccg gtaaaagcga ttaagccccc
     5341 aactcctgca gtacgcgcat catgggtaca taaaacgggg gcgaccggcg gatttggtag
     5401 ctatgtcgcg tttattccag aaaaagagct gggtatcgtg atgctggcta acaaaaacta
     5461 tcccaatcca gcgagagtcg ccgccgcctg tcagattctc aacgctctac agtaaaattt
     5521 catcgggtcc gaattttcgg accttttctg cgcttttcct tgctgtcatc tacacttaga
     5581 aaaaaaccag taaggaaaca tttatgcgcc tgctccctct cgttgccgca gcgacagctg
     5641 catttctggt cgttgcctgc agttctccta cgccgccgcg tggcgtgaca gtagtgaata
     5701 atttcgacgc taaacgctat ctcggtacct ggtatgagat tgcccgtttt gatcaccgtt
     5761 ttgaacgtgg actggaaaaa gtcaccgcaa catacagtct gcgtgatgac ggcggcctga
     5821 atgtcatcaa taaaggctat aaccctgaca gagaaatgtg gcagcagagt gaagggaaag
     5881 cgtactttac cggcgcacca acacgcgctg cgctgaaagt gtcattcttt ggtcctttct
     5941 atggcggtta taacgtcatt gcacttgatc gggaataccg ccatgcgctg gtttgcggcc
     6001 cggaccgcga ctacctgtgg atactctccc gcacgccaac catttctgac gaagtgaaac
     6061 aggagatgct ggcagtcgcg acccgggaag ggtttgatgt cagtaagttt atttgggtac
     6121 agcagcccgg tagttagtga gtgctgagtt tcagaccaat aatccccaat acgattaacg
     6181 ccagactcgc caggcgcatc gggttagcgg actcaccgag cagcacaatg ccggtgatgg
     6241 ccgcgccgac tgcgccaata cccgtccaca cggcataagc cgtccctacc ggtaacgatt
     6301 tcatcgccca ggcaagtagc gccatactga ctatcatcgc cgtcacagta ataacacttg
     6361 gcgtcaaacg actaaagccg tgggtatatt tcaggccaac ggcccatacc acttccagca
     6421 gaccagcaat aactaagata atccaggaca tatcaggctc cggagaattg gggccgtccc
     6481 cggtgaaaag aggcgtttgc aggtcgtcct gcaaagctga tggttgaacg gatattttgc
     6541 caggtggcag gggaatttca actattacct ttgtgactat cgcaaaaaaa caaacggcac
     6601 gacataactg tcgtaccgtt attgcttatt gctgcgcttt cgttgctgca ccagaaatcg
     6661 cgttaccgcc atcagaaatg tcttcaccaa cgccacgcgt ggtgttgcag gcagttaata
     6721 ctgttgaaag caccagaaca gaaaagatcg ctgcaattgt cttcttcacc ataacgtctt
     6781 ccttttaggt aatgttattt atgtttgcct atagcaaatt aataatagac aacatcgcca
     6841 aaaatgatgg aaatctgatg atttttcgga agagaagaaa aattagctgg cagcgcggga
     6901 gatggagttg ccgagatgtt tgatgtcttc gccgaaaccg cgagcggtgt tacagcccgt
     6961 gagcagcgtg ctggcaagca aaacaagaac gataaggcgt ttcatcatct ctgacctgaa
     7021 taagtgatgg tgcagcctgc aggccgcacc acaaccgcat tacttcacgc gagagacgta
     7081 ttcaccagag cgggtatcca ctttgatgac ttcgccgatt tgtacgaaca gcggaacttt
     7141 aaccacagcg ccagtagaca gggtagccgg tttgccgcca gtacctgcgg tatcaccttt
     7201 caggcccgga tcggtatcaa cgatttccag ttcaacgaag ttcggcggag taacggagat
     7261 cggctgacca ttccacagag ttacgataca ctctgcctga tccagcagcc atttagcgtt
     7321 gtcgccaatt gcttttgcat cagcagacag ctgctcgaaa gtttcgttgt tcatgaagtg
     7381 ccagaactca ccgtcgttgt acaggtaagt caggttcata tcgacaacat cagcgccttc
     7441 agcggaatca gtagatttga aggttttttc tacgcgagta ccggtcagca gacgacgcag
     7501 tttaacgcga gcaaatgcct ggcctttacc cggttttacg aattcactcg cttcaaccgc
     7561 gtaaggttcg ccgtctaaca tgattttaag accagcacga aaatcgttgc tatagtacgt
     7621 tgccataagg ccctctgaaa tttgttaatt ggtagctaag ccacaaaatg gcgcatattg
     7681 taaccctaaa taccccatcc agagaagatt ggttaacgca acttgccgat gttgtgaccg
     7741 atcctggtga acttctgcgt cttttgaata tagacgcgga cgaaaaactg ttggccggac
     7801 gcagcgccaa aaagctgttt gccctgcgtg tgccccgctc atttatcgat cgcatggaga
     7861 aaggcaatcc ggacgatcct attttgcgtc aggtacttac ctcgcaagat gagtttgtcg
     7921 tcgcgtccgg attctccacc gacccactgg aagaacagca cagcgtagtg cctggtttgt
     7981 tgcataaata ccacaaccgg gcgcttttgc tggtcaaagg cggctgcgcg gtaaattgcc
     8041 gctattgctt ccgtcgccac ttcccctatg ccgaaaatca gggcaacaag cgtaactggc
     8101 agactgcact tgagtatgtt gctgcgcatc cggaactgga cgagatgatt ttctccggcg
     8161 gcgatccgct gatggcgaaa gatcacgagc tggactggtt gctcacacaa ctggaagcca
     8221 tcccgcatat caaacgtctg cggattcaca gccgtctgcc gattgtgatc ccggcacgta
     8281 tcaccgaggc gctggttgaa cgctttgccc gttctacgct gcaaatcttg ctggtgaatc
     8341 acatcaatca tgccaatgag gtagatgaaa cgttccgtca ggcgatggct aaattgcgcc
     8401 gtgtcggtgt caccctgctt aaccagagcg ttctgttacg tggtgtgaac gataacgcac
     8461 aaacgttggc aaacctgagt aatgcgttgt tcgatgccgg cgtgatgccg tattacctgc
     8521 atgtgctaga taaagtacag ggcgcggcgc attttatggt gagtgatgac gaagcacggc
     8581 agattatgcg tgagttgctg acattggtgt cgggatatct ggtgccgaaa ctggcgcgag
     8641 aaattggcgg cgaacccagc aaaacgccgc tggatctcca gctacgccag cagtaactga
     8701 ttaaacaggg aagttaaaca ttagcctgat gcgctacgct tatcaggcct acattgtgca
     8761 ttgaaattga atggatttgc aatgaactgt aggccggata aggcgtttac gccgcatccg
     8821 gcaagagcaa caaacgcaag tacagcaacc tgtcattaaa ctgggaaatt taatctttgc
     8881 ccgttgcata acctttaatt attcatatac attgctgttg ttactcttta tatagccaac
     8941 accaataatt aacctttctt ttctgtttat atagagtgag tacggctatt tatgttattt
     9001 taacaactat tactaccaag cccatttatc tggcttataa attaaaatta attatttaat
     9061 tgagaaatgg ttagggagaa cctacatggc cataagcatc aagggtgtta ataccggtgt
     9121 tattcgcaag agtaataatt tcattgcact ggcgctgaaa attaaagaac ctcgcaataa
     9181 agaatcgttg ttctttatgt ctgtcatgga actgcgcgac ctgctgattg cgctggaaag
     9241 tcgtctgcat caaaagcata aactgaatgc tgctgcacgc cttcagtatg agcaagctcg
     9301 cgataaagtc attaagaaaa tggcagaaaa catcccagaa attctggttg atgagcttaa
     9361 aaatgccgat attaatcgtc gggttaacac gctggaactt accgataatc aaggcgaaaa
     9421 tcttaccttc gtattaacgc tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt
     9481 gcagattgaa atgctggcgc gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga
     9541 actggcatta cgtattacct cactgttaga tttcctgccg ttatacgacg ttgactgcca
     9601 ggacaatggc aatctggaat atgacaccta ttcccaaccg gaatggaaac ataacctgtt
     9661 cgaccattat ctggcggtgc tttaccgctt taaagatgaa agtggcaaag aacaatttag
     9721 cggcacagtg gtgaaaacgc gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg
     9781 ccgaatgctt gatttcagcc cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta
     9841 tgttcgcacc gtagcggcga ataacgccca accgctaacc caggatcaat gcttacgcgc
     9901 gctgcatcac ttacgtgttc aatccaccag caaaactgca ccacaggcga aataatctca
     9961 tctttgctaa tgcgaggctg gagccatcca gcctgaaatg actgataacg tgatcgttgg
    10021 tcatgccgga tgcgacgtaa acgccttatc cggcctacaa attctcaagc attcaatcag
    10081 ttgcaatgcc tgctgtaggc ctgataagcg aagcgcatca ggcaataata cgtttgtcac
    10141 ccagcctctg gatcacatca gttcgggcac ttataaacct gaccgataat ctggctatcc
    10201 gtcgggacaa aactggacaa cattccctgc gacgggctgc tgatgccata aatcacgtta
    10261 ccgcccattg cagccgcctg gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc
    10321 tcttctccgt gttgcccgga aagccagttg ctttgcttac ctgtcgcagt accaatcagc
    10381 tggcactctg cgcctggctg ctcgtccaca atgcgtacac tctgaccggc agcactcaat
    10441 tcgttgctgg agctacaacc cgccatcagt agcgcggcac cgacaatccc tgctaagtat
    10501 tttacgtgca tgttattccc cataatcaat gagctggacg ccactcgcgt cgtccgtgtc
    10561 tgaatcttat actagaaaga tgaccaaaag aaaaaccccc agacatttct gcccgggggt
    10621 ttgtttattt ctgcgaggtg cagggcaatt acatcatgcc gcccatgcca cccatgccgc
    10681 ccataccgcc agcagcgcct aagtcagctg catcattttt cggcaggtcg gtaaccatgc
    10741 actcggtggt gatcatcagg ccagccacag aagccgcgta ctgcagagca gaacgggtta
    10801 ctttggttgg gtccaggata cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt
    10861 tgtaaccgta gttgccgtcg ccgcctttaa cggtgttagc aacaacagac ggttcttcgc
    10921 cgcagttcag gacgatctga cgcagcggag cttccattgc acgcagtgca actttgatac
    10981 ccacgttctg gtcttcgttc tgaccacgca ggtcagccag tttagacgct acgcggatca
    11041 gcgcaacacc accaccagca accacgcctt cttctaccgc agcacgggtc gcgtgcaggg
    11101 cgtcttcaac gcgtgctttt ttctctttca tttcaacttc ggtagcagca cctactttga
    11161 taactgcaac gccgcctgcc agtttcgcta cgcgttcctg cagtttttca cggtcgtagt
    11221 cagaagttgc ttcttcaatc tgctgacgga tctgagcaac acggccctgg attgcagctt
    11281 cttcacccac gccatcgatg atagtggtgg tgtctttgtt gatcacaaca cgtttagcct
    11341 gacccaggtc ttccagggtt gctttttcca gttccatacc gatctcttca gagatcacgg
    11401 taccgccagt cagggttgcg atatcctgca gcatagcttt acgacgatcg ccgaagcccg
    11461 gagctttaac tgcagcaact ttcacgatgc cacgcatggt gttaacaacc agagttgcca
    11521 gcgcttcgcc ttctacatct tcagcgatga tcagcagcgg tttgcctgct ttggcaacgg
    11581 cttccagaac cggcagcatt tcgcggatgt tggagatttt cttgtcagcc agcaggatga
    11641 acgggctttc cagttctact gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt
    11701 agccacggtc gaactgcata ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt
    11761 cttcaacggt gataacgcct tctttaccga ctttgtccat cgcttcagcg atcagtttac
    11821 ctacggtttc gtcggagtta gcggagatgg taccaacctg agcaatcgct ttagagtcag
    11881 agcacggtac ggacagcgct ttcagttctt caactgcagc ggtaacggct ttgtcgatac
    11941 cacgtttcag gtccatcggg ttcatgcccg cagcaacagc tttcagaccc tcagtgatga
    12001 tagcctgagc cagtacagtt gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttag
    12061 aggcaacttc tttcaccatc tgcgcaccca tgttttcgaa cttgtcttcc agttcgattt
    12121 cacgagcaac ggaaacacca tctttggtga tggtcggtgc accgaaagat ttatccagaa
    12181 ctacgttacg gcctttcgga ccgagggtaa ctttcactgc atctgccagt acgtttacgc
    12241 cgcgcagcat tttcacacga gcgtcgttac cgaattttac gtctttagct gccattatct
    12301 ttattcctta aattcgtatg ttcagtgtcg tgcgaggatt acgcttcaac aattgccaga
    12361 atgtcgcttt cggacatgat caacacttct tcattgtcga tcttctcaga tttcacaccg
    12421 tagccatcgt tgaaaataac gatgtcgcca actttcacat ccagcggctt cacttcgcca
    12481 ttttcaagga tacggccatt gccgacagcc agcacttcgc cgcgggtgga tttagccgct
    12541 gcagagccgg tcagaacgat gccgccagca gatttagttt caacttcttt acgcttgacg
    12601 atcacgcgat catgcaatgg acgaatattc attgataact ctcctttgag aaagtccgta
    12661 tctgttatgg gtgacgccgg agcttacgtg gtttcccggc tggtgaccag agaaatgggg
    12721 atgaggattc gccccttcaa gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg
    12781 gtgaaaaccc tgattcacct cacatttcat cgcaatttct tcatcgccgt cataagcgaa
    12841 tctgattgtg ctaccatcga aaatctacgc agttgcccaa aatttgggcg caatcggata
    12901 tcaacccatg agtggactca aacaagaact ggggctggcc cagggcattg gcctgctatc
    12961 gacgtcatta ttaggcaccg gcgtgtttgc cgttcctgcg ttagctgcac aggtagcagg
    13021 caataacagc ctgtgggcgt ggcccgtttt gattatctta gtgttcccga ttgcgattgt
    13081 gtttgcgatt ctgggtcgcc actatcccag cgcaggcggt gtcgcgcact tcgtcggtat
    13141 ggcgtttggt tcgcggcttg agcgagtcac cggctggttg tttttatcgg tcattcccgt
    13201 gggtttgcct gccgcgctac aaattgccgc cgggttcggc caggcaatgt ttggctggca
    13261 tagctggcaa ctgttgttgg cagaactcgg tacgctggcg ctggtgtggt atatcggtac
    13321 tcgcggtgcc agttccagtg ctaatctaca aaccgttatt gccggactta tcgtcgcgct
    13381 gattgtcgct atctggtggg cgggcgatat caaacctgcg aatatcccct tcccggcacc
    13441 tggtaatatc gaacttaccg ggttatttgc tgcgttatca gtgatgttct ggtgttttgt
    13501 cggtctggaa gcatttgccc atcttgcctc ggaatttaaa aatccagagc gtgattttcc
    13561 tcgcgcattg atgattggtc tgctgctggc aggattagtc tactggggct gtacggtagt
    13621 cgtcttacac ttcgacgcct atggtgaaca aatggccgcg gcagcatcgc ttccaaaaat
    13681 tgtagtgcaa ctgttcggtg taggagcgtt atggattgcc tgcgtaattg gctatctgac
    13741 ctgctttgcc agtctcaaca tttatataca gagcttcgcc cgcctggtct ggtcgcaggc
    13801 gcaacataat cctgaccact acctggcacg cctctcttct cgccatatcc cgaataatgc
    13861 cctcaatgcg gtgctcggct gctgcgtggt gagcactttg gtgattcatg ctttagagat
    13921 caatctggac gctcttatta tttatgccaa tggcatcttt attatgattt atctgttatg
    13981 catgctggca ggctgtaaat tattgcaagg acgttatcga ctactggcgg tggttggcgg
    14041 gctgttatgc gttctgttac tggcaatggt cggctggaaa agtctctatg cgctgattat
    14101 gctggcgggg ttatggctgt ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac
    14161 ataatccggc gtttcgacat taatcctagc gatcgtcttt atgatcaagg cggtcgcggt
    14221 catcatcctt tcgctggtac tcaccatcaa aagtattacc gccaccggtc ccggcgctaa
    14281 aaccgccgcc tggcatgcga gaaaagcgca aatgtggcat caacttcact gtcagatgct
    14341 tttgcaccgg cggcaataaa agcagaagac cgaggaagtc ggtgaaaaag cccggtaata
    14401 aaagcagcaa accggcaata atcagcgaaa cacttttaat catctccgcc gcagggtttt
    14461 cacccgccgc cattttttgc tgcatcagca cgaaattctt aaagccctgg ttacgtacca
    14521 gtgacatacc gataactgac gtgaatataa ccagcacgag ggtcagcaat acccccaata
    14581 catgggcaac ctgaataaag attgaaatct caatatagac ataaaggaaa atggcaataa
    14641 aaggtaacca gcgcaaaggt ttctcctgta atagcagccg gttaaacccg gctacctgaa
    14701 tgggttgcga atcgcgttca gcttatattg tggtcattag caaaatttca agatgtttgc
    14761 gcaactattt ttggtagtaa tcccaaagcg gtgatctatt tcacaaatta ataattaagg
    14821 ggtaaaaacc gacacttaaa gtgatccaga ttacggtaga aatcctcaag cagcatatga
    14881 tctcgggtat tcggtcgatg caggggataa tcgtcggtcg aaaaacattc gaaaccacat
    14941 atattctgtg tgtttaaagc aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa
    15001 caacattcgt atcgaagaag atctgttggg taccagggaa gttccagctg atgcctacta
    15061 tggtgttcac actctgagag cgattgaaaa cttctatatc agcaacaaca aaatcagtga
    15121 tattcctgaa tttgttcgcg gtatggtaat ggttaaaaaa gccgcagcta tggcaaacaa
    15181 agagctgcaa accattccta aaagtgtagc gaatgccatc attgccgcat gtgatgaagt
    15241 cctgaacaac ggaaaatgca tggatcagtt cccggtagac gtctaccagg gcggcgcagg
    15301 tacttccgta aacatgaaca ccaacgaagt gctggccaat atcggtctgg aactgatggg
    15361 tcaccagaaa ggtgaatatc agtacctgaa cccgaacgac catgttaaca aatgtcagtc
    15421 cactaacgac gcctacccga ccggtttccg tatcgcagtt tactcttccc tgattaagct
    15481 ggtagatgcg attaaccaac tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga
    15541 catcctgaaa atgggtcgta cccagctgca ggacgcagta ccgatgaccc tcggtcagga
    15601 attccgcgct ttcagcatcc tgctgaaaga agaagtgaaa aacatccaac gtaccgctga
    15661 actgctgctg gaagttaacc ttggcgcaac agcaatcggt actggtctga acacgccgaa
    15721 agagtactct ccgctggcag tgaaaaaact ggctgaagtc actggcttcc catgcgtacc
    15781 ggctgaagac ctgatcgaag cgacctctga ctgcggcgct tatgttatgg ttcacggcgc
    15841 gctgaaacgc ctggctgtga agatgtccaa aatctgtaac gacctgcgct tgctctcttc
    15901 tggtccacgt gccggcctga acgagatcaa cctgccggaa ctgcaggcgg gctcttccat
    15961 catgccagct aaagtaaacc cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt
    16021 catcggtaac gacaccactg ttaccatggc agcagaagca ggtcagctgc agttgaacgt
    16081 tatggagccg gtcattggcc aggctatgtt cgaatccgtt cacattctga ccaacgcttg
    16141 ctacaacctg ctggaaaaat gcattaacgg catcactgct aacaaagaag tgtgcgaagg
    16201 ttacgtttac aactctatcg gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa
    16261 cggtgacatc gtgggtaaaa tctgtgccga aaccggtaag agtgtacgtg aagtcgttct
    16321 ggaacgcggt ctgttgactg aagcggaact tgacgatatt ttctccgtac agaatctgat
    16381 gcacccggct tacaaagcaa aacgctatac tgatgaaagc gaacagtaat cgtacagggt
    16441 agtacaaata aagaaggcac gtcagatgac gtgccttttt tcttgtgagc agtaacttaa
    16501 aaataacaat ctaatatcaa cttgttaaaa aacaaggaag gctaatatgc tagttgtaga
    16561 actcatcata gttttgctgg cgatcttctt gggcgccaga ttggggggaa taggtattgg
    16621 ttttgcaggc ggattggggg tgctggttct tgccgctatt ggcgttaaac ccggtaacat
    16681 cccgttcgat gtcatttcca ttatcatggc ggttatcgcc gctatttctg ccatgcaggt
    16741 tgctggcggt ctggactatc tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa
    16801 atacatcaca atcctcgcac cgatcgtgac ctatttcctg acaatctttg ctggtactgg
    16861 caacatctct ctggcgacac tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa
    16921 accttgccgt ccgctgtcta ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc
    16981 aatctcagcg gcagtggttt acatgtcttc cgtgatggaa ggtcatggca tcagctacct
    17041 ccatctgctc tccgtggtca tcccgtccac cctgctggcg gttctggtga tgtccttcct
    17101 ggtcactatg ctgttcaact ccaaactctc tgacgatccg atttatcgca agcgtctgga
    17161 agagggcctg gttgaactgc gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac
    17221 gtccgtctgg ctgttcctgc tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag
    17281 cccaagcatg ggtctggttg aaaaaccgct gatgaacacc accaacgcaa tcctgatcat
    17341 catgctcagc gttgcaactc tgaccaccgt tatctgtaaa gtcgataccg acaacatcct
    17401 caactccagc accttcaaag caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg
    17461 gctgggcgat actttcgttt ccaacaacat cgactggatc aaagataccg ctggtgaagt
    17521 gattcagggt catccgtggc tgctggccgt catcttcttc tttgcttctg ctctgctgta
    17581 ctctcaggct gcaaccgcaa aagcactgat gccgatggct ctggcactga acgtttctcc
    17641 gctgaccgct gttgcttctt tcgctgcggt gtctggtctg ttcattctgc cgacctatcc
    17701 gacgctggtt gctgcggtac agatggatga cacgggtaca acccgtatcg gtaaattcgt
    17761 cttcaaccat ccgttcttca tcccgggtac tctgggtgtt gccctggccg tttgcttcgg
    17821 cttcgtgctg ggtagcttca tgctgtaatg accaatcgcg gggcgttcac gccccgcttt
    17881 ctttcccgcc gactaacatc cttcccccgt ccgttgtata gtgacctctc tcttgcggtt
    17941 ccatctgttc ttgcgaggtg tttatgcttg atgaaaaaag ttcgaatacc gcgtctgtcg
    18001 tggtgctatg taccgcaccg gatgaagcga cagcccagga tttagccgcc aaagtgctgg
    18061 cggaaaaact ggcggcctgc gcgaccttga tccccggcgc tacctctctc tattactggg
    18121 aaggtaagct ggagcaagaa tacgaagtgc agatgatttt aaaaactacc gtatctcacc
    18181 agcaggcact gctggaatgc ctgaagtctc atcatccata tcaaaccccg gaacttctgg
    18241 ttttacctgt tacacacgga gacacagatt acctctcatg gctcaacgca tctttacgct
    18301 gatcctgcta ctttgcagca cttccgtttt tgccggatta ttcgacgcgc cgggacgttc
    18361 acattttgtc cccgcggatc aagcctttgc ttttgatttt cagcaaaacc aacatgacct
    18421 gaatctgacc tggcagatca aagacggtta ctacctctac cgtaaacaga tccgcattac
    18481 gccggaacac gcgaaaattg ccgacgtgca gctgccgcaa ggcgtctggc atgaagatga
    18541 gttttacggc aaaagcgaga tttaccgcga tcggctgacg cttcccgtaa ccatcaacca
    18601 ggcgagtgcg ggagcgacgt taactgtcac ctaccagggc tgtgctgatg ccggtttctg
    18661 ttatccgcca gaaaccaaaa ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc
    18721 acagcctgtg tctgttccgc agcaagagca gcacaccgcg caattgccct tttccgcgct
    18781 ctgggcgttg ttgatcggta ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc
    18841 actgatttct ggcatcgtgc tgggtggtaa acagcggctc tccactgcca gagcattgtt
    18901 gctgaccttt atttatgtgc aggggatggc actgacttac acggcgctgg gtctggtagt
    18961 tgccgccgca gggttacagt tccaggcggc gctacagcac ccatacgtgc tcattggcct
    19021 cgccatcgtc tttaccttgc tggcgatgtc aatgtttggc ttgtttactc tacaactccc
    19081 ctcttcgctg caaacacatc tcacattgat gagcaatcgc caacagggcg gctcacctgg
    19141 cggtgtgttt gttatggggg cgattgccgg actgatctgt tcaccatgca ccaccgcacc
    19201 gcttagcgcg attctgctgt atatcgccca aagcgggaac atgtggctgg gcggcggcat
    19261 gctttatctc tatgcgttgg gcatgggcct gccgctgatg ctaattaccg tctttggtaa
    19321 ccgcttgctg ccgaaaagcg gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt
    19381 gatcctcgca ctgccgatct tcctgctgga gcgagtgatt ggtgatatat ggggattacg
    19441 cttgtggtcg gcgctgggtg tcgcattctt tggctgggcc tttatcacca gcctacaggc
    19501 caaacgcggc tggatgcgcg tggtgcaaat aatcctgctg gcagcggcat tggttagcgt
    19561 gcgcccactt caggattggg catttggtgc gacgcatacc gcgcaaactc agacgcatct
    19621 caactttaca caaatcaaaa cggtagatga gttaaatcag gcgctcgttg aagccaaagg
    19681 caaaccggtg atgttagatc tttatgccga ctggtgcgtc gcctgtaaaa agtttgagaa
    19741 atacaccttc agcgacccgc aggtgcaaaa agcgttagca gacacggtct tacttcaggc
    19801 caacgtcact gctaacgacg cacaagatgt ggcgctgtta aagcatctta atgtccttgg
    19861 actaccgaca attctctttt ttgacggaca aggccaggag catccacaag cacgcgtcac
    19921 gggctttatg gatgctgaaa ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac
    19981 acttgcagtg ggaaagacgg aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc
    20041 cctgaaatta ttagaattac aagggattgc caacaccacg ctggagatgg ttgctgaacg
    20101 tgtggattat ccactggacg agctacgccg cttctggcca gacaaagagg caatcctcta
    20161 cgatgcgctg cgttatctta gccaacagat agatgtctgg cgtcgtcagc tgatgcttga
    20221 cgaaacgcaa accgccgaac aaaagctgct ggcacgttat caggcgttat cggagtgcgt
    20281 taaaaacaac cgctatccgg gctgtctatt tatcgctgcc tgtacgtttt atcccgaccc
    20341 tggccaccct attcatcaac tggccgatca gcaaaaaagc gcggcctacg atttcaccca
    20401 cgaactgtta accacgctgg aagttgacga tccggcgatg gtagcaaagc agatggaact
    20461 ggtgctggaa ggctgtttaa gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac
    20521 cgcacatcgg ctggcggaag atatcctgcg cttcgcccgc tgccgccagg gtggtgcact
    20581 gacctgacag aaacacagaa aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt
    20641 ttactgaaat taggttgacg agatgtgcag attacggttt aatgcgcccc gttgcccgga
    20701 tagctcagtc ggtag
//
LOCUS       DAESHI010000378        11551 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.guided.130, whole genome shotgun sequence.
ACCESSION   DAESHI010000378 DAESHI010000000
VERSION     DAESHI010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11551
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.guided.130"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            1..652
                     /locus_tag="KW011_004636"
                     /pseudo
     CDS             1..652
                     /locus_tag="KW011_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            649..1263
                     /locus_tag="KW011_004637"
     CDS             649..1263
                     /locus_tag="KW011_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HBH7662752.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="KW011_004638"
     CDS             complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="KW011_004638"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBH7662753.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3038..3496
                     /gene="gpt"
                     /locus_tag="KW011_004639"
     CDS             3038..3496
                     /gene="gpt"
                     /locus_tag="KW011_004639"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBH7662754.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3588..4832
                     /gene="frsA"
                     /locus_tag="KW011_004640"
     CDS             3588..4832
                     /gene="frsA"
                     /locus_tag="KW011_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBH7662755.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4890..5291
                     /gene="crl"
                     /locus_tag="KW011_004641"
     CDS             4890..5291
                     /gene="crl"
                     /locus_tag="KW011_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBH7662756.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5330..6385)
                     /gene="phoE"
                     /locus_tag="KW011_004642"
     CDS             complement(5330..6385)
                     /gene="phoE"
                     /locus_tag="KW011_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBH7662757.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6673..7776
                     /gene="proB"
                     /locus_tag="KW011_004643"
     CDS             6673..7776
                     /gene="proB"
                     /locus_tag="KW011_004643"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBH7662758.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7788..9041
                     /gene="proA"
                     /locus_tag="KW011_004644"
     CDS             7788..9041
                     /gene="proA"
                     /locus_tag="KW011_004644"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBH7662759.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9156..9231
                     /locus_tag="KW011_004645"
     tRNA            9156..9231
                     /locus_tag="KW011_004645"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9189..9191,aa:Thr,seq:cgt)
     gene            9837..9995
                     /locus_tag="KW011_004646"
     CDS             9837..9995
                     /locus_tag="KW011_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662760.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         3000 a         2685 c         2914 g         2952 t
ORIGIN      
        1 acaccccatt ataaggggta cgaataaagt aacggggcag tctattttac agcggcatat
       61 tgcctcgttt tcgcatcatg gtttgcctga aggcagtgac gacgcgctac ggtatattgc
      121 ggaacatgac gatgcgctgg cgtttgcgcg tattaatcgc cagctgatct ctttacgcat
      181 aatgcaacag gttaaggcca ccggaagtcc ggttctggat gtggcgcata actttgttag
      241 cgcgtgtcaa atcggcgatc aacagggctg gttgcatcgt aaaggtgcca cgccggatga
      301 caacggtctg gtgattattc ccggttcact cggtgattac tcctagcttg tacagcctgt
      361 tgcgaacgaa aaaacgctcc actcgctggc gcatggggct gggcgtaaat gggggcgcac
      421 cgagtgtaaa gggcgtctgg cagcgaaata cacagcgacg cagctctcac gtactgaact
      481 tggcagtcgg gtaatttgtc gcgataaaca actcatcttt gaagaagcgc cacaagccta
      541 taaatcggct gaaagcgtgg tgcaatgtct ggtgcaggct ggtttaatta ttcctgtcgc
      601 gcggctgcgt ccggtgctaa cgctcaaaaa cagtggaggg aaaaaaggat gatcttgcta
      661 caactctcct cagcgcaggg gccggaagaa tgttgtctcg cagtgaaaaa ggcgctggag
      721 cgattaatta aagaagctgc ccgacaagac gtcgcggtaa cggtgttgga aacagaaacg
      781 ggccgctact ctgacacgct gcgttcagct ctggtttctc tggatggcga taacgcatgg
      841 gcattaagcg aaagctggtg cggcactatt cagtggattt gtccgagtcc gtatcggcct
      901 catcatgggc gcaaaaactg gtttctgggc attgggcgtt ttaccgctga tgagcaggaa
      961 caatcggatg caatccgtta tgagacgttg cgttcgtcgg ggccgggcgg tcaacatgtc
     1021 aataaaaccg actcggcggt acgcgccacg catttggcat ccggtattag cgtgaaggtt
     1081 cagtcagagc gtagtcagca tgctaacaag cggctggcac gattgctgat tgcctggaag
     1141 ctggagcagc agcaacagga aaatagcgcg gtgctgaaat cgcagcggcg aatgttccat
     1201 caccagattg aacgtggcaa cccgagacgg acatttacag ggatggcttt tatcgaagga
     1261 taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat
     1321 tacttcgccg gaatttcttt cagcagttca gtcagcagtg tccagtaatg acctacgctt
     1381 tcgatgtgaa cttgctcatc cggagagtgt ggaccggtga tagttggccc gatagaaacc
     1441 atgtccattt ccggatacgg ttttttgaac agaccacatt ccaggcccgc gtggataatc
     1501 tggatgttcg gcgttttgtt gaacagacgc tgataggttt cacgtaccag atgcatcacc
     1561 ggagaattag cgtccggctg ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca
     1621 gccagtttac ccagcgaatc cagcatgctc accacgtagt ctttaccgct gtcgatcaga
     1681 gaacggatca gacagtgaat ttctacgtta ttatcagtca tggtcaccac accgacgttc
     1741 agggaggttt cgaccacacc ttttgccacg tctgagttgc ggatcacacc gttcggggtg
     1801 gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc tttatcgttc
     1861 gctacagagt ccagcaacaa ggccagattt ttctctttct ctgccagctc gtttttcagg
     1921 atctcctgat aggtattcac cagagatttc aggacgtcga ctttatcagc tgcgacagca
     1981 atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccgccgtt gaaatcgata
     2041 aggcgcagat ccagttcttc tgcatgaccc gctaggaagc gcaccagcag tttgttggca
     2101 ttacccaggc caacgtggat ttccccgccg gagtgacccc ctttcagacc ttttaaggtt
     2161 aacttgaagg tttcaaaacc agctggaacc gcttcacgat ctaaatgcag gttggaggtg
     2221 aagtcgatac cccccgcaca acccatgtag atttcacctt cttcttcgga gtcggtgtta
     2281 atcagaatat cagcctgcaa ccagttgctc tgtaagccga acgcaccgtc cataccggct
     2341 tcttcggtca tggtcagcag cacttccagc gggccgtgaa ccacgttttc gtcagccaga
     2401 accgccagcg cagacgccat accaatgccg ttatctgcac ccagcgtggt gccgcgcgct
     2461 ttaacccatt cgccatcaat ataaggctgg ataggatctt tcgtgaagtc atgcacggtg
     2521 tcgttatttt tctgcggcac catatcgagg tgggcctgta agacgaccga tttacgattt
     2581 tccatacctg cggtagcagg tttacgaatc aggatattac ctacctgatc gcgttcgaca
     2641 tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag
     2701 gacgggtgag gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat
     2761 tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt
     2821 cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtttgagaat
     2881 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     2941 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     3001 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     3061 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     3121 atggaaaggc attattgccg taagccgtgg cggtctggta ccgggtgcgt tactggcgcg
     3181 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     3241 gcgcgagctt aaagtgctaa aacgcgcaga aggcgatggc gaaggcttca tcgttattga
     3301 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
     3361 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
     3421 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     3481 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     3541 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     3601 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     3661 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
     3721 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgtg
     3781 aaatcctcga cgtccaggcg cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
     3841 tatacgatac ggtgattggc taccgtggtg gcaactggat ttatgagtgg gccatccagg
     3901 cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc gcaactcagt ggtcgtcact
     3961 ggctgcatgc ggctacgttg tacaacattg ctgcctatcc tcatctgaaa ggagatgacc
     4021 tggccgagca agcgcaggct ttgtcaaacc gtgcctatga agaagccgcc cagcgtctgc
     4081 cgggcacgat gcggcagatg gagtttaccg tacccggcgg tgcgcccatc accggctttt
     4141 tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt aatgtgtggt ggtctggatg
     4201 cgatgcagac ggactattac agcctgtatg aacgttattt tgcgccgcgc ggcattgcga
     4261 tgctgactat tgatatgccg tcggtgggct tttcatcaaa atggaagctc acccaggact
     4321 ccagcctgtt gcatcagcac gttttaaagg ctctgcctaa cgtaccgtgg gtggatcaca
     4381 ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt tgccgtgcgt ctggcatatc
     4441 ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc ggtagttcat accctgttga
     4501 gtgattttaa gtgccagcaa caggtgccgg aaatgtatct tgacgttctg gcgagtcgtt
     4561 tggggatgca tgatgcttcc gacgaagcgt tacgcgtgga gctgaatcgc tattcattaa
     4621 aagtgcaagg attgctgggg cgtcgctgcc caacgccaat gttatcaggc tactggaaga
     4681 acgatccgtt cagcccggaa gaggactcac gcttaatcac ctcatcatct gctgacggta
     4741 aattattaga gatcccattt aacccggtgt atcggaattt tgacaaaggt cttcaggaaa
     4801 tcaccggctg gatcgaaaaa cgcttgtgtt aaaaatttgc taaaatttgc caatttggta
     4861 aaacagttgc atcacaacag gagattgcaa tgacgttacc gagtggacac ccgaagagca
     4921 gattgatcaa aaaatttacc gcactaggcc cgtatattcg tgaaggtaag tgcgaagata
     4981 atcgattctt tttcgattgt ctggctgtat gcgtcaacgt gaaaccggca ccggaagtgc
     5041 gtgagttctg gggctggtgg atggagcttg aagcgcagga atcccgtttt acctacagtt
     5101 accagtttgg tctgttcgat aaagcaggcg actggaagag tgttccggta aaagacactg
     5161 aagtggttga acgactggag cacaccctac gtgagtttca cgagaagctg cgtgaactgc
     5221 tgacgacgct gaatctgaag ctggaaccgg cggatgattt tcgtgatgaa ccggtgaagt
     5281 taacggcgtg agtgaaatgt gccggatgta tcacatccgg caatatccat taaaactgat
     5341 aggtcatgcc aaccgcgaca atatcatcat tattaatatt caatttgtta tcgctatcca
     5401 gttggttgat tttataatca acaaacgctg acatattttt gttgaaataa tacgtagcac
     5461 cgacgtcgat ataattgacc agatcttcat caccgatacc ttcaatatct ttccctttcg
     5521 ataagacata acccagcgat ggacgcagac caaagtcaaa ctggtattga gcgaccgctt
     5581 caaagttctg tgtcttattg gcaaagccgc cagttattgg cgtcattttg cgtgtttcag
     5641 aatagaaagt tgccagataa atattattgg catcgtattt cagacctgtt gcccatgctt
     5701 ctgcacgctt gcctgtgcca cggctttgca ggttctgctc gttggtgcga tctgagttgg
     5761 tataggcccc actaatggcg aaatcgctgc cgccaaagtc atatgtcaat gacgtgccga
     5821 agccatcgcc gttttgcttt ttaacgtcgc ggttttcgtt tttcccttga tattgcaggg
     5881 ttaagttcag gccatcgata acgccgaaga agtcggtgtt ccgatacgtc gccagaccgc
     5941 tggcgcgttt ggtcataaag ttgtcggtct gcgccgagga atcgccacca aattccggga
     6001 acatatcggt ccaggcttcc acgtcataca acgcgcccag gttacgacca taatcgaaag
     6061 aacccaaatc tttatatttc aacccggcaa aagcgagacg cgttttctgc tgtgcagtat
     6121 cactctccgc tttatttccg gcaaactccg cttcccaccg accataacca gtcagttgat
     6181 cgttaatttg tgtttcacct ttaaaaccaa aacggatata actctggtcg ccatctttac
     6241 tggcgttatc actcatataa tgcatggctt taactttgcc atagacatcc agtttattac
     6301 cgtctttatt atatatttct gcagcctgta cagatgcaga tgccacaatg cccatcacca
     6361 ctaatgccag agtgctcttt ttcattttca ttcctgattt taattaacgc gcgaatattc
     6421 agcgggagag ccccgttgaa aacaggaaag tttttaacct gagattgtta aagatatatt
     6481 acagattaat aatattctta aaatgtggta atttattaaa tctgtaataa aagcgtaaac
     6541 aactgccgct aggcttgctg atcccgcgca acaaaacgcc atgctttgct cacagatggt
     6601 tggcaaccga cgacagtcct gctaaaacgt tcgtttgata tcatttttcc taaaattgaa
     6661 tggcagagaa tcatgagtga cagccagacg ctggtggtaa aactcggcac cagtgtgcta
     6721 acaggcggat cgcgccgtct gaaccgtgcc catattgttg aacttgttcg ccagtgcgcg
     6781 cagttacatg ccgccgggca tcggattgtt attgtgacgt cgggcgcgat cgccgccgga
     6841 cgtgagcacc tgggttaccc ggaactgcca gcgactatcg cctcgaaaca actgctggca
     6901 gcggtagggc agagtcgact gattcaactg tgggaacagc tgttttcgat ttatggcatt
     6961 cacgtcgggc aaatgctgct gacccgtgct gatatggaag accgtgaacg cttcctgaac
     7021 gcccgcgaca ccctgcgtgc gttgctcgat aacaatatcg ttccggtaat caatgagaac
     7081 gatgctgtcg ctacggcaga gattaaagtc ggcgataacg ataacctttc tgcgctggcg
     7141 gcgattctgg cgggtgccga taaactgttg ctgctgaccg atcaaaaagg tttgtatacc
     7201 gctgacccgc gcagcaatcc gcaggcagaa ctgattaaag atgtttacgg cattgatgac
     7261 gcactgcgcg cgattgccgg tgacagcgtt tcaggcctcg gaactggcgg catgagtacc
     7321 aaattgcagg ccgctgacgt ggcttgccgt gcgggtatcg acaccattat tgccgcgggc
     7381 agcaagccgg gcgttattgg tgatgtgatg gaaggcattt ccgtcggtac gctgttccat
     7441 gcccaggcga ctccgcttga aaaccgtaaa cgctggattt tcggtgcgcc gccggcgggt
     7501 gaaatcacgg tagatgaagg ggcaactgcc gccattctgg aacgcggcag ctccctgttg
     7561 ccgaaaggca ttaaaagcgt gactggcaat ttctcgcgtg gtgaagtcat ccgcatttgc
     7621 aacctcgaag gccgcgatat cgcccacggc gtcagtcgtt acaacagcga tgcattacgc
     7681 cgtattgccg gacaccactc gcaagaaatt gatgcaatac tgggatatga atacggcccg
     7741 gttgccgttc accgtgatga catgattacc cgttaaggag ctggccgatg ctggaacaaa
     7801 tgggcattgc tgcgaagcaa gcctcgtata aattagcgca actctccagc cgcgaaaaaa
     7861 atcgcgtgct ggaaaaaatc gccgatgaac tggaagcaca aagcgaaatc atcctcaacg
     7921 ctaacgccca ggatgttgct gacgcgcgag ccaatggcct tagcgaagcg atgcttgacc
     7981 gtctggcact gacgcccgca cggctgaaag gcattgccga cgatgtacgt caggtgtgca
     8041 acctcgccga tccggtgggg caggtaatcg atggcggcgt actggacagc ggcctgcgtc
     8101 ttgagcgtcg tcgcgtaccg ctgggggtta ttggcgtgat ttatgaagcg cgcccgaacg
     8161 tgacggttga tgtcgcttcg ctgtgcctga aaaccggtaa tgcggtgatc ctgcgcggtg
     8221 gcaaagaaac gtgtcgcact aacgctgcaa cggtggcggt gattcaggac gccctgaaat
     8281 cctgcggctt accggcgggt gccgtgcagg cgattgataa tcctgaccgt gcgctggtca
     8341 gtgaaatgct gcgtatggat aaatacatcg acatgctgat cccgcgtggt ggcgctggtt
     8401 tgcataaact gtgccgtgaa cagtcgacaa tcccggtgat cacaggtggt ataggcgtat
     8461 gccatattta cgttgatgaa agtgtagaga tcgctgaagc attaaaagtg atcgtcaacg
     8521 cgaaaactca gcgtccgagc acatgtaata cggttgaaac gttgctggtg aataaaaaca
     8581 tcgccgatag cttcctgccc gcattaagca aacaaatggc ggaaagcggc gtgacattac
     8641 acgcagatgc agctgcactg gcgcagttgc aggcaggccc tgcgaaggtg gttgctgtta
     8701 aagccgaaga gtatgacgat gagtttctgt cattagattt gaacgtcaaa atcgtcagcg
     8761 atcttgacga tgccatcgcc catattcgtg aacacggcac acaacactcc gatgcgatcc
     8821 tgacccgcga tatgcgcaac gcccagcgtt ttgttaacga agtggattcg tccgctgttt
     8881 acgttaacgc ctctacgcgt tttaccgacg gcggccagtt tggtctgggt gcggaagtgg
     8941 cggtaagcac acaaaaactc cacgcgcgtg gcccaatggg gctggaagca ctgaccactt
     9001 acaagtggat cggcattggt gattacacca ttcgtgcgta aataaaaccg ggtgatgcaa
     9061 aagtagccat ttgattcaca aggccattga cgcatcgccc ggttagtttt aaccttgtcc
     9121 accgtgattc acgttcgtga acatgtcctt tcagggccga tatagctcag ttggtagagc
     9181 agcgcattcg taatgcgaag gtcgtaggtt cgactcctat tatcggcacc attaagaaaa
     9241 ttaataactt agcttaactt ctctaagaaa ttgtatcttc tgttatcact tacaaacgcc
     9301 tatacgttgt tatgggcttt ggtagtgtaa cacgtatggg cgagtagttc ccaggctcaa
     9361 cctcgtaaca agttcctttg aagctggaag acgtgccacc gtctctaaac cttctaccaa
     9421 aactcttagc aagatgttcc acacctggcg cggtgtccaa tgatgctaca cgtcgtgaga
     9481 cgttgcagag gataaacagg ctaaatgatg ggaagagttt taagtgcaat agtggattaa
     9541 agcccacctc ttcaaaacct aacttgatcg ggaaagaatc aagctctagc acggtttcgt
     9601 ccaccggagt acaacgggtg cctgataaga gaagaactgc taagacttca tagcttttgt
     9661 gaaaaagccc tttttagtgg atacgcctac ggcgcttatt ttttggtata accatcaaag
     9721 gcgctttatg gctcctattg tcttgaaaat gtatctttac ttgtattgtg ttttatgcgt
     9781 agtctccctg tgtagtatgt aacttgttaa ttttcatatg aatacagagg cacacgatga
     9841 aaaacattgc agcaatcaaa cgtaataacc gcaagattca cgctcgtaag ttcctgtcta
     9901 cgccagaagg aaaagcctgg ctagagcgta aacagagaga gaacgaagaa agaaaactcc
     9961 ttagtgagtt gaaatggctt agagaggatt tctgaaaatc tccagaacgc aacaggacgc
    10021 attctaacgc tgttttcatt actcggtata gggataggtg atgtgtgttt aatgcgtctg
    10081 tgcgcttgtt ttgtgcctta ttttgcattt tcataatgcg gaagagaaca cggaaaacct
    10141 taaaattaat tattggggta tttaaggcta ttttcaggtg atttaagggt attaaacttg
    10201 attaaaatat aaacagataa ttatgtatct tattgattta taaggttatt tttaagtaaa
    10261 ggtaaataaa aaataaatag ccataaatag ctataaatac atcttaatac ctatatccct
    10321 tcccttatgg ctagattgcg agaggtgctt aacgcgattt tcagcgcccc ccgatctgga
    10381 aaatccagaa tccccaaata tcagcaagga attttcttgc gtaatcaaca tcgtttttac
    10441 ctattaaatc attacacaga aagcctttct gtaatgtctc tcactaaact ttcactaaac
    10501 acgagaatga tcggttttat gtcggatcag atgctagaaa cagcaccacg tctcacaagg
    10561 gctgtaagcg atgaaaccag tgtttatgcg ggtgcaggtc aaaatatagg ccaaaatcca
    10621 cttaacataa taaatgttat atgtgctaag gaacacatat aacgccttga attgatgtat
    10681 caggggaaaa gtgatggatt ttttacgtag gagcacgaaa gggcttctat tgacttttgt
    10741 atgctatgaa atataattaa agttttttgt ttttattgtg tgatgtggtg ggttacacag
    10801 gggaatagat taaatggtgt tctgtttata caggtttatt tgttagatca acaatttggc
    10861 atttctaaac agatgtttga acgttgccaa atgtcttgac aacacaagaa aagggcttgc
    10921 tatgccaggc attacacgaa ataaggctat aaaaacaaaa taaattttaa aatttgcaca
    10981 aacgagaaac tcgttcagaa acaagcagaa caatttttag ccaacaattt ctgatggtgc
    11041 tctgtaagcc gcattacaca aggctttgcg aaagtgatgt aaaagtgaag aaagtaagaa
    11101 ttgaactttt aagctacttt tcattggatt ttttagtagg gaattacaca gggaatagag
    11161 agcattattc tatgatttac acagggaata tagggggatt atggcacaca catagaaaga
    11221 cattacacag agtgctatgt gacaaatgga tgtaacgaga ggttacattc atttatctga
    11281 aatgctctct gtaagaaagt tggtagtaac gatatgttac atagactaat tattgaatgt
    11341 aacgatatta cacatcggta gtaacgatac gttacatcgg aagatgattg gatgtaacgg
    11401 tagctcacag tgaactaggg gtaggtcata gtgaactacc cccacagaat tctgttaccc
    11461 ctatcaagtc tgatacccct atcaaaactg atacccgata agtataaata acacctaaat
    11521 agctgcgcgg aatagtagat cactttgagg g
//
LOCUS       DAESHI010000379         6819 bp    DNA     linear   BCT 08-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate 19ER2648
            SAMN19976108-rid12605643.guided.176, whole genome shotgun sequence.
ACCESSION   DAESHI010000379 DAESHI010000000
VERSION     DAESHI010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN19976108
            Sequence Read Archive: SRR15011082
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001084051.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001084051.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/07/2021 20:28:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,655
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,316
            CDSs (with protein)               :: 4,316
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 112 of 246
            Pseudo Genes (incomplete)         :: 73 of 246
            Pseudo Genes (internal stop)      :: 83 of 246
            Pseudo Genes (multiple problems)  :: 22 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6819
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN19976108-rid12605643.guided.176"
                     /isolate="19ER2648"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="New Zealand"
                     /lat_lon="40.90 S 174.88 E"
                     /collection_date="2019-07-12"
                     /collected_by="ESR"
     gene            1..837
                     /locus_tag="KW011_004647"
     CDS             1..837
                     /locus_tag="KW011_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HBH7662761.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(944..1348)
                     /locus_tag="KW011_004648"
                     /pseudo
     CDS             complement(944..1348)
                     /locus_tag="KW011_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(1392..1781)
                     /locus_tag="KW011_004649"
     CDS             complement(1392..1781)
                     /locus_tag="KW011_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662762.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            1999..2439
                     /locus_tag="KW011_004650"
     CDS             1999..2439
                     /locus_tag="KW011_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HBH7662763.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(3195..3344)
                     /locus_tag="KW011_004651"
     CDS             complement(3195..3344)
                     /locus_tag="KW011_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH7662764.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            4533..5348
                     /gene="sul2"
                     /locus_tag="KW011_004652"
     CDS             4533..5348
                     /gene="sul2"
                     /locus_tag="KW011_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HBH7662765.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            5409..5936
                     /locus_tag="KW011_004653"
                     /pseudo
     CDS             5409..5936
                     /locus_tag="KW011_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            5957..6430
                     /locus_tag="KW011_004654"
     CDS             5957..6430
                     /locus_tag="KW011_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HBH7662766.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            6505..6780
                     /locus_tag="KW011_004655"
                     /pseudo
     CDS             6505..6780
                     /locus_tag="KW011_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
BASE COUNT         1676 a         1782 c         1763 g         1598 t
ORIGIN      
        1 atgttcatgc cgcctgtttt tcctgctcat tggcacgttt cgcaacctgt tctcattgcg
       61 gacacctttt ccagcctcgt ttggaaagtt tcattgccag acgggactcc tgcaatcgtc
      121 aagggattga aacctataga agacattgct gatgaactgc gcggggccga ctatctggta
      181 tggcgcaatg ggaggggagc agtccggttg ctcggtcgtg agaacaatct gatgttgctc
      241 gaatatgccg gggagcgaat gctctctcac atcgttgccg agcacggcga ctaccaggcg
      301 accgaaattg cagcggaact aatggcgaag ctgtatgccg catctgagga acccctgcct
      361 tctgcccttc tcccgatccg ggatcgcttt gcagctttgt ttcagcgggc gcgcgatgat
      421 caaaacgcag gttgtcaaac tgactacgtc cacgcggcga ttatagccga tcaaatgatg
      481 agcaatgcct cggaactgcg tgggctacat ggcgatctgc atcatgaaaa catcatgttc
      541 tccagtcgcg gctggctggt gatagatccc gtcggtctgg tcggtgaagt gggctttggc
      601 gccgccaata tgttctacga tccggctgac agagacgacc tttgtctcga tcctagacgc
      661 attgcacaga tggcggacgc attctctcgt gcgctggacg tcgatccgcg tcgcctgctc
      721 gaccaggcgt acgcttatgg gtgcctttcc gcagcttgga acgcggatgg agaagaggag
      781 caacgcgatc tagctatcgc ggccgcgatc aagcaggtgc gacagacgtc atactagata
      841 tcaagcgact tctcctatcc cctgggaaca catcaatctc accggagaat atcgctggcc
      901 aaagccttag cgtaggattc cgccccttcc cgcaaacgac cccaaacagg aaacgcagct
      961 gaaacgggaa gctcaacacc cactgacgca tgggttgttc aggcagtact tcatcaacca
     1021 gcaaggcggc actttcggcc atccgccgcg ccccacagct cgggcagaaa ccgcgacgct
     1081 tacagctgaa agcgaccagg tgctcggcgt ggcaagactc gcagcgaacc cgtagaaagc
     1141 catgctccag ccgcccgcat tggagaaatt cttcaaattc ccgttgcaca tagcccggca
     1201 attcctttcc ctgctctgcc ataagcgcag cgaatgccgg gtaatactcg tcaacgatct
     1261 gatagagaag ggtttgctcg ggtcggtggc tctggtaacg accagtatcc cgatcccggc
     1321 tggccgtcct ggccgccaca tgaggcatgt tccgcgtcct tgcaatactg tgtttacata
     1381 cagtctatcg cttagcggaa agttctttta ccctcagccg aaatgcctgc cgttgctaga
     1441 cattgccagc cagtgcccgt cactcccact cttacattgt tcgcagtatc taggtacatg
     1501 ttattgcgtg caccttcatg atgtataatg ttattaatga cagcagaaag atcgacagaa
     1561 ttaatattct tcgtacctgg gacagttatc gggtgtcggc cagtgatgag accaaaggat
     1621 gcagtataag atgaaccact actattatca tggccaccat taccaccacc gttaaaacta
     1681 cctcccgaac ttccagagcc gctatgacca tcattgtagg ggtcatttgg tcccatgtga
     1741 ccaaaaccac cgacagtacc gctatttaac tctgtactca taaaattatc ctctttgact
     1801 tttaaaaaca gcaaaaactg tatttataac aatgtgttta tatacagtat tcgaaatact
     1861 acgcccgtca aaaccacacc gtcaacaggt tttttgtgta tttttcacgc ctcacgggca
     1921 gcctcagacc ttttgtgtta aaacagtaat atgttctgct gttacaatac ggtacacaca
     1981 ggagacactg tcacttttat ggcatggcac acagttcaag agacactgaa attaacagga
     2041 aagtcgcgct cgcaattata tcgtgacatg gctaaaggcc ttgtcagcta tcgcacagga
     2101 aaaaatgatc gccgggagtt cgagaccagt gaactgattc gggcctatgg cgagctgcgc
     2161 acttctgaga caccaaaaag gcacaaggag ggacactcca aatctgtcgt tagccaggcg
     2221 gaaaatgctc agctagacgt cattcagcag aaactggacg ctctgcagca gactgttacc
     2281 ctaatgcttg aagacaagat ggcgcgggaa gcagaaaacc gcctgcgaaa tgaggagagg
     2341 gaacaactac aggccgaaat tagccgtctg gctgacgcgc tggcacagga aaaaaagcgg
     2401 ggattctggt cgcggctgtt tcgctccggt tcagagtaac agtgcaggtg actctgattt
     2461 accataacat ctgttatcag caccgcctga gaggccctca gggcgtttct gacgggaaac
     2521 cggtaaaaca ggcaacaaac cacccgaaaa cccgccagaa acgcgcaggc tctgttttta
     2581 cggcatgcat gagtatgcgc acaagtgcat agcgcggaaa ttcacaccat tcagccgggg
     2641 taagtcctgt ttccggcgtt cttcccatcc gaaaacggtg aacggggacg caatttattg
     2701 cgtggccgtg aagcgttgcc ggcgcagccg gaaaacccgc tgtcagcacg ttcaggaagg
     2761 gggtgatgta caaatggcac agaggatgaa agtgacgtat gagctgaagg aatgatgtaa
     2821 aagtgatgta taagtgatgt aaaagtgatg taaaagtgat gtatgaactg aaggagcgat
     2881 gtaattctgt agtcaggaag aataacccgt tcatgcaggc atgaacgata cagtgaggaa
     2941 cgcttttttt gtcacgatct ccccttaacc tgcgtaaatc acccgccatg gttgattatt
     3001 tttcaacctc aaaatggtca tacacctaac gcagccgcga ttttcatcgc aactgcatga
     3061 attaaaagaa aaaaaaggaa tatttcgttt tcacgagtaa tacccgggta ttaaaaatcc
     3121 actgtaatta catgaaaata aagaaataaa aggtaatacc ggagtattac cacgtacgcg
     3181 ggcaagcgaa aaagctataa cgcttcccgg ccgacaggcg acccaaaatc cacaggggca
     3241 tgcaggttct cttcagtaac ctgcgccatt aaatcaaaaa ggtcatattc gccgggaagc
     3301 tgcgtatcag tcgaatccag ttgttcatca tattcagatg gcatggggtg atatccagta
     3361 gctgagaggt tgttagcggg tttcggggcg cagccctgaa ccagtcatgt agcgctagcg
     3421 gagtgtatac tggcttacta tgttgccacg attgcgaaaa tcagtgaagt gcttcatgtg
     3481 gcaggagaaa aaaggcagca ccggcgcgtc agcagaatat gcgatacagg agatattccg
     3541 cttcctcgct cactgactcg ctacgctcgg tcgttcggct gcggcgagcg gtaccggctt
     3601 acggacgggg acggaaattt cctggaagat accaggaaga cacttagcaa agaagagata
     3661 aggccacggc gaagccgttt ttccataggc tccgcccccc tgacaagcat cgcaaaatct
     3721 gacgctcaaa tcagtggtgg cgaaacccga caggactata aagatcccag gcgtttcccc
     3781 ctggtagctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc
     3841 tgttatggcc gcggtttctc attccacgcc tgacactcag ttccgtgaag gcagttcgct
     3901 ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc ttgtccggta
     3961 actatcaact tgagtccaac ccggaaagac acgacaaaac gccactggca gcagccatta
     4021 gtaacaggag tgcacgagaa gatagattaa gaggtcttga agtagaggcc tgactgcggc
     4081 tacactgaaa gaacagtttt ggtaatggcg gtctcgtgaa ccctgttacc gggttaagca
     4141 gttccccaac tgacttaacc ttcgaacaaa ccgcctcccc aggcggtttt ttcgtttaca
     4201 gagcaagaga ttacgattca ttgaaaagga tctcaagaag atcctttgta gattactatc
     4261 aattaatctt ccgaacatga caccagtcac tgttgattac ggttaaaccg ctccgtaaca
     4321 ggtttccctc gtattggtga cttgcagcac caaagaagca aaaaagataa atatatctac
     4381 ttattgagag atgatatatt tatttccaaa atgctacaga tacatcatca tgaaacggat
     4441 caccgcaaac aggttactcg cctggggatt ccctttcgac ccgagcatcc gtatgagact
     4501 catgctcgat tattattatt atagaagccc ccatgaataa atcgctcatc attttcggca
     4561 tcgtcaacat aacctcggac agtttctccg atggaggccg gtatctggcg ccagacgcag
     4621 ccattgcgca ggcgcgtaag ctgatggccg agggggcaga tgtgatcgac ctcggtccgg
     4681 catccagcaa tcccgacgcc gcgcctgttt cgtccgacac agaaatcgcg cgtatcgcgc
     4741 cggtgctgga cgcgctcaag gcagatggca ttcccgtctc gctcgacagt tatcaacccg
     4801 cgacgcaagc ctatgccttg tcgcgtggtg tggcctatct caatgatatt cgcggttttc
     4861 cagacgctgc gttctatccg caattggcga aatcatctgc caaactcgtc gttatgcatt
     4921 cggtgcaaga cgggcaggca gatcggcgcg aggcacccgc tggcgacatc atggatcaca
     4981 ttgcggcgtt ctttgacgcg cgcatcgcgg cgctgacggg tgccggtatc aaacgcaacc
     5041 gccttgtcct tgatcccggc atggggtttt ttctgggggc tgctcccgaa acctcgctct
     5101 cggtgctggc gcggttcgat gaattgcggc tgcgcttcga tttgccggtg cttctgtctg
     5161 tttcgcgcaa atcctttctg cgcgcgctca caggccgtgg tccgggggat gtcggggccg
     5221 cgacactcgc tgcagagctt gccgccgccg caggtggagc tgacttcatc cgcacacacg
     5281 agccgcgccc cttgcgcgac gggctggcgg tattggcggc gctgaaagaa accgcaagaa
     5341 ttcgttaact gcacattcgg gatatttctc tatattcgcg cttcatcaga aaactgaagg
     5401 aacctccatt gaatcgaact aatatttttt ttggtgaatc gcattctgac tggttgcctg
     5461 tcagaggcgg agaatctggt gattttgttt ttcgacgtgg tgacgggcat gccttcgcga
     5521 aaatcgcacc tgcttcccgc cgcggtgagc tcgctggaga gcgtgaccgc ctcatttggc
     5581 tcaaaggtcg agatgtggct tgccccgagg tcatcaactg gcaggaggaa caggagggtg
     5641 catgcttggt gataacggca attccgggag taccggcggc tgatctgtct ggagcggatt
     5701 tgctcaaagc gtggccgtca atggggcagc aacttggcgc tgttcacagc ctatcggttg
     5761 atcaatgtcc gtttgagcgc aggctgtcgc gaatgttcgg acgcgccgtt gatgtggtgt
     5821 cccgcaatgc cgtcaatccc gacttcttac cggacgagga caagagtacg ccgcagctcg
     5881 atcttttggc tcgtgtcgaa cgagagctac cggtgcggct cgaccaagag cgcaccgtta
     5941 acccaggatg agaaccttga aagtatcatt gatagctgcg aaagcgaaaa acggcgtgat
     6001 tggttgcggt ccagacatac cctggtccgc gaaaggggag cagctacttt ttaaagcatt
     6061 gacctacaat cagtggcttc tggtgggtcg caagacgttt gaatctatgg gcgcactccc
     6121 caataggaaa tacgcggtcg ttacccgctc aggttggaca tcaaatgatg acaatgtagt
     6181 tgtatttcag tcaatcgaag aggccatgga caggctagct gaattcaccg gtcacgttat
     6241 agtgtctggt ggcggagaaa tttaccgaga aacattaccc atggcctcta cgctccactt
     6301 atcgacgatc gacatcgagc cagaggggga tgttttcttc ccgagtattc caaatacctt
     6361 cgaagttgtt tttgagcaac actttacttc aaacattaac tattgctatc aaatttggaa
     6421 aaagggttaa caaagctatg caatcgacgg caaaaagctt cgttcgcttc gcgcactacg
     6481 cctttttccg cgattgatag cgacgatatg gttgtttgcc atggtgatcc ctgcatgccg
     6541 aacttcatgg tggaccctaa aactcttcaa tgcacgggtc tgatcgacct tgggcggctc
     6601 ggaacagcag atcgctatgc cgatttggca ctcatgattg ctaacgccga agagaactgg
     6661 gcagcgccag atgaagcaga gcgcgccttc gctgtcctat tcaatgtatt ggggatcgaa
     6721 gcccccgacc gcgaacgcct tgccttctat ctgcgattgg accctctgac ttggggttga
     6781 tgttcatgcc gcctgttttt cctgctcatt ggcacgttt
//
